gebhart/patrepeval huggingface dataset:
Outgrowing endothelial and smooth muscle cells for tissue engineering approaches (2017)

In recent years, circulating progenitors of endothelial cells and smooth muscle cells were identified in the peripheral blood. In our study, we evaluated the utilization of both cell types isolated and differentiated from peripheral porcine blood in terms for their use for tissue engineering purposes. By means of density gradient centrifugation, the monocyte fraction from porcine blood was separated, split, and cultivated with specific culture media with either endothelial cell growth medium-2 or smooth muscle cell growth medium-2 for the differentiation of endothelial cells or smooth muscle cells. Obtained cells were characterized at an early stage of cultivation before the first passage and a late stage (fourth passage) on the basis of the expression of the antigens CD31, CD34, CD45, nitric oxide synthase, and the contractile filaments smooth-muscle alpha-actin (sm-alpha-actin) and smoothelin. Functional characterization was done based on the secretion of nitric oxide, the formation of a coherent monolayer on polytetrafluoroethylene, and capillary sprouting. During cultivation in both endothelial cell growth medium-2 and smooth muscle cell growth medium-2, substantially two types of cells grew out: early outgrown CD45-positive cells, which disappeared during further cultivation, and in 85% (n = 17/20) of cultures cultivated with endothelial cell growth medium-2 colony-forming late outgrowth endothelial cells. During cultivation with smooth muscle cell growth medium-2 in 80% (n = 16/20) of isolations colony-forming late outgrowth smooth muscle cells entered the stage. Cultivation with either endothelial cell growth medium-2 or smooth muscle cell growth medium-2 had selective effect on the late outgrown cells to that effect that the number of CD31-positive cells increased from 34.8% ± 13% to 83.9% ± 8% in cultures cultivated with endothelial cell growth medium-2 and the number of sm-α-actin+ cells increased from 52.6% ± 18% to 88% ± 5% in cultures cultivated with smooth muscle cell growth medium-2, respectively. Functional analyses revealed significantly higher levels of nitric oxide secretion, endothelialization capacity, and capillary formation in not expanded cultures cultivated with endothelial cell growth medium-2 in comparison to later stages of cultivation and mature aortic cells. Blood seems to be a reliable and feasible source for the isolation of both endothelial and smooth muscle cells for application in tissue engineering approaches. Whereas, early co-cultures of early and late outgrowth cells provide functional advantages, the differentiation of cells can be directed selectively by the used culture medium for the expansion of highly proliferative late outgrowth endothelial cells and late outgrowth smooth muscle cells, respectively.
Optically pumped semiconductor lasers: a new reliable technique for realizing highly efficient visible lasers (2005)

Optically pumped semiconductor material is a complimentary gain medium for rare earth or transition metal doped crystals. The design of several compositions based on GaAs allows the realization of a wavelength range between 710nm and 1180nm. This can be diode pumped and frequency doubled to cover the near UV up to the yellow spectral range. The power is scaleable and we have realized several Watts at 488nm and 460nm. Experimental results will be presented and discussed as well as reliability data to show that this technology has ripened for industrial applications.
Front Matter: Volume 9547 (2015)

This PDF file contains the front matter associated with SPIE Proceedings Volume 9547, including the Title Page, Copyright information, Table of Contents, Invited Panel Discussion, and Conference Committee listing.
SPiCE: a web-based tool for sequence-based protein classification and exploration (2014)

SPiCE is an easy-to-use online tool for extracting and exploring sequence-based features of sets of proteins, allowing non-experts to apply advanced classification techniques. The tool is available at http://helix.ewi.tudelft.nl/spice.
Supplementary Figure 2 from Tumor-Promoting Desmoplasia Is Disrupted by Depleting FAP-Expressing Stromal Cells (2023)

&lt;p&gt;The adaptive immune response plays a critical role in FAP-CAR T cell-mediated anti-tumor activity in immunogenic AE17.OVA tumors.&lt;/p&gt;
Executive functions after orbital or lateral prefrontal lesions: Neuropsychological profiles and self-reported executive functions in everyday living (2012)

It was demonstrated that LPFC damage is particularly prone to cause cognitive executive deficit, while OFC injury is more strongly associated with self-reported dysexecutive symptoms in everyday living. The study illustrates the challenge of identifying executive deficit in individual patients and the lack of strong anatomical specificity of the currently employed methods. There is a need for an integrative methodological approach where standard testing batteries are supplemented with neuropsychiatric and frontal-specific rating scales.
Color Cherenkov imaging of clinical radiation therapy (2021)

Color vision is used throughout medicine to interpret the health and status of tissue. Ionizing radiation used in radiation therapy produces broadband white light inside tissue through the Cherenkov effect, and this light is attenuated by tissue features as it leaves the body. In this study, a novel time-gated three-channel camera was developed for the first time and was used to image color Cherenkov emission coming from patients during treatment. The spectral content was interpreted by comparison with imaging calibrated tissue phantoms. Color shades of Cherenkov emission in radiotherapy can be used to interpret tissue blood volume, oxygen saturation and major vessels within the body.
Growth of a self-assembled monolayer by fractal aggregation (1992)

Atomic force microscope images show that self-assembled monolayers of octadecyltrichlorosilane form on mica by nucleating isolated, self-similar domains. With increasing coverage, the fractal dimension of the growing domains evolves from 1.6 to 1.8. At higher coverage, continued growth is limited by adsorption from solution. Monte Carlo simulations that include, for the first time, adsorption as well as surface diffusion qualitatively reproduce both the growth kinetics and evolution of fractal structure, much better than a two-dimensional diffusion-limited-aggregation model.
Paired APTTs of low and high lupus anticoagulant sensitivity permit distinction from other abnormalities and achieve good lupus anticoagulant detection rates in conjunction with dRVVT (2018)

The percent correction of the APTT reagent pair showed higher values in LA-positive samples. The combination will be useful with respect to differentiating LA from other abnormal samples and is effective in LA detection when paired with dRVVT.
Characterization and modeling of emitter-base leakage in high speed SiGe NPNs (2008)

Leakage through the base is a common yield detractor in SiGe NPNs. The defects are commonly referred to as dasiapipespsila and are manifested as a current path between emitter and collector independent of base bias. In this article we discuss pipes which have been observed due to retarded base growth. Advanced light/thermally induced voltage alterations (LIVA) and cross section transmission electron microscopy (XTEM) were used to identify these defects, which resulted in leakage paths through the base to the collector. The pipes were found to have resistances ranging 100-200 kOmega, and could be modeled as junction field-effect transistors (JFETs) in which the pipes serve as the channel and the base as the gate electrode. Technology computer-aided design (TCAD) was utilized to model the pipes and provide insight into their behavior. In particular, the higher CE leakage currents of NPNs containing a selective collector implant are explained by changes in the conduction band resulting from the SIC implant. This correlates with observed NPN yield trends.
The Effect of Changing From Whole-Cell to Acellular Pertussis Vaccine on the Epidemiology of Hospitalized Children With Pertussis in Canada (2006)

With ACV-P, pertussis hospitalizations in children 4-59 months old decreased in frequency, consistent with improved vaccine effectiveness, but remained prominent among very young infants. Improved control strategies are needed to reduce infections among infants too young for pertussis vaccination.
S-Sulfonation: a Reversible Chemical Modification ofHuman Immunoglobulins Permitting Intravenous Application (1983)

The generation and release of mediators of inflammation and anaphylaxis via activation of complement or of Fc receptor-bearing cells is held responsible for adverse reactions observed upon intravenous administration of standard immunoglobulins. They are caused by immunoglobulin G (IgG) effector functions predominantly located in the Fc region of the molecule and ‘activated’ by aggregate formation. Their functional activity depends on the correct conformation of the Cy2 domains of Fc and are therefore impaired or even abrogated by S-sulfonation of the hinge disulfide bonds, as demonstrated in this communication: S-sulfonated IgG (S-IgG) has no anticomplementary activity and does not interact with Fc-receptors anymore. After antigen binding, i. e. immune complex (IC) formation, sulfonated IgG is about half as potent as standard IgG in complement activation or phagocyte stimulation (human monocytes and granulocytes). The two activities synergize, however, so that in the presence of complement S-IgG IC are as effective phagocyte activators as standard IC. Moreover, S-sulfonation being the only chemical modification of imunoglobulins that is reversible, it can be demonstrated that all IgG effector functions important for antigen removal are regained by reconstitution of the disulfide bonds.
1,3-Dipolar Cycloadditions to Baylis-Hillman Adducts: Rationale for the Observed Diastereoselectivity (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT1,3-Dipolar Cycloadditions to Baylis-Hillman Adducts: Rationale for the Observed DiastereoselectivityRita Annunziata, Maurizio Benaglia, Mauro Cinquini, Franco Cozzi, and Laura RaimondiCite this: J. Org. Chem. 1995, 60, 15, 4697–4706Publication Date (Print):July 1, 1995Publication History Published online1 May 2002Published inissue 1 July 1995https://pubs.acs.org/doi/10.1021/jo00120a009https://doi.org/10.1021/jo00120a009research-articleACS PublicationsRequest reuse permissionsArticle Views569Altmetric-Citations42LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Data-Driven Control Design for Neuroprotheses: A Virtual Reference Feedback Tuning (VRFT) Approach (2004)

This paper deals with design of feedback controllers for knee joint movement of paraplegics using functional electrical stimulation (FES) of the paralyzed quadriceps muscle group. The controller design approach, virtual reference feedback tuning (VRFT), is directly based on open loop measured data and fits the controller in such a way that the closed-loop meets a model reference objective. The use of this strategy, avoiding the modeling step, significantly reduces the time required for controller design and considerably simplifies the rehabilitation protocols. Linear and nonlinear controllers have been designed and experimentally tested, preliminarily on a healthy subject and finally on a paraplegic patient. Linear controller is effective when applied on small range of knee joint angle. The design of a nonlinear controller allows better performances. It is also shown that the control design is effective in tracking assigned knee angle trajectories and rejecting disturbances.
Effects of firing angle imbalance on 12-pulse rectifiers with interphase transformers (2002)

Firing angle imbalance between six-pulse groups of a 12-pulse rectifier leads to current imbalance, due to the finite magnetizing inductance of the interphase transformer. The amount of current imbalance is limited by the load regulation of the six-pulse groups. This paper presents a quantitative analysis of this effect and others, based on appropriate models of the system. Equations are developed which predict steady-state current shifts and transient behavior using an averaged model. A piecewise-linear model of the system is used to verify and extend the results via computer simulation. Results are normalized, making them useful for design purposes.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Path Computation Element - Rendre le réseau IP WAN programmable (2018)

Dans des réseaux de plus en plus complexes, l'ingénierie de trafic distribuée semble atteindre ses limites. L'ingénierie de trafic centralisée basée sur le Path Computation Element est-elle une réponse à ces problématiques ?
Upconversion with ultrabroad excitation band: Simultaneous use of two sensitizers (2007)

The authors demonstrate the ability to combine sensitizers effectively working with single emitter in order to increase the excitation window for noncoherent upconversion. They show effective upconversion of the red part of the sun spectrum realized by ultralow excitation intensity (as low as 1Wcm−2) and ultrabroad excitation spectrum (Δλ∼80nm).
The Impact of Damping on Flicker Frequency Noise of AlN Piezoelectric MEMS Resonators (2017)

This paper presents a detailed study on the effect of damping on flicker frequency (1/f) noise of 1.1-GHz aluminum nitride (AlN) contour mode resonators (CMRs). A total of 52 different AlN CMRs with different sizes, electrode designs, and anchor types are systemically designed and fabricated to give a wide range of quality factors (Q) from 300 to 3500, allowing the study of how two major damping mechanisms in AlN CMRs: 1) anchor losses; and 2) thermoelastic damping affect the resonator 1/f noise. In total, we have measured 1/f noise of 104 CMRs using the modified homodyne noise measurement setup and the results confirm that 1/f noise shows a clear power law dependency ranging from 1/Q <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2.7</sup> to 1/Q <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3.5</sup> , depending on the main nature of the damping mechanism. Additionally, we have measured 1/f noise of 25 CMRs at 10 K and the results follow the trend observed at ambient temperature. Understanding and accounting for the effect of damping on 1/f noise are crucial for building ultra-low noise microelectromechanical systems resonators for sensing, timing, and frequency applications.
Analysis of aquifer tests to determine hydrologic and water-quality conditions in stratified-drift and riverbed sediments near a former municipal well, Milford, New Hampshire (1996)

Sea level: In this report, "sea level" refers to the National Geodetic Vertical Datum of 1929 a geodetic datum derived from a general adjustment of the first-order level nets of the
Comparative analysis of fixed-vocabulary communication acceleration techniques (1987)

Principles involved in the formation of acceleration vocabularies are reviewed followed by an analysis of alternate strategies for implementing abbreviation expansion accelleration techniques. Data regarding the coverage power of most-frequently-used-word sets, the effects of word length and keystroke analyses on calculated word set efficiencies, and the effect of different termination and abbreviation strategies are presented. Word set analyses which do not take keystroke analysis into account are shown to be overly optimistic due to the heavily skewed distribution of word length versus usage. A generic abbreviation algorithm is described which allows implementation and comparison of other strategies. Fixed-vocabulary, letter-based word prediction techniques are shown to be a form of cued abbreviation expansion with a relatively low motor efficiency.
Small-Molecule Inhibition of UBE2T/FANCL-Mediated Ubiquitylation in the Fanconi Anemia Pathway (2019)

The Fanconi anemia pathway orchestrates the repair of DNA interstrand cross-links and stalled replication forks. A key step in this pathway is UBE2T and FANCL-dependent monoubiquitylation of the FANCD2-FANCI complex. The Fanconi anemia pathway represents an attractive therapeutic target, because activation of this pathway has been linked to chemotherapy resistance in several cancers. However, to date, very few selective inhibitors of ubiquitin conjugation pathways are known. By using a high-throughput screen-compatible assay, we have identified a small-molecule inhibitor of UBE2T/FANCL-mediated FANCD2 monoubiquitylation that sensitizes cells to the DNA cross-linking agent, carboplatin.
Adeno-associated virus serotypes 9 and rh10 mediate strong neuronal transduction of the dog brain (2013)

Canine models have many advantages for evaluating therapy of human central nervous system (CNS) diseases. In contrast to nonhuman primate models, naturally occurring canine CNS diseases are common. In contrast to murine models, the dog's lifespan is long, its brain is large and the diseases affecting it commonly have the same molecular, pathological and clinical phenotype as the human diseases. We compared the ability of four intracerebrally injected adeno-associated virus vector (AAV) serotypes to transduce the dog brain with green fluorescent protein as the first step in using these vectors to evaluate both delivery and efficacy in naturally occurring canine homologs of human diseases. Quantitative measures of transduction, maximum diameter and area, identified both AAV2/9 and AAV2/rh10 as significantly more efficient than either AAV2/1 or AAV2/5 at transducing cerebral cortex, caudate nucleus, thalamus and internal capsule. Fluorescence co-labeling with cell-type-specific antibodies demonstrated that AAV2/9 and AAV2/rh10 were capable of primarily transducing neurons, although glial transduction was also identified and found to be more efficient with the AAV2/9 vector. These data are a prerequisite to evaluating the efficacy of recombinant AAV vectors carrying disease-modifying transgenes to treat naturally occurring canine models in preclinical studies of human CNS disease therapy.
Advanced motion control by multi‐sensor‐based disturbance observer (2006)

Abstract Motion control has been widely used in industry applications. One of the key technologies of motion control is a disturbance observer, which quarries a disturbance torque of a motion system and realizes a robust acceleration control. The disturbance observer can observe and suppress the disturbance torque within its bandwidth. Recent motion systems have begun to spread in society and are required to have the ability to make contact with the unknown environment. Such a haptic motion requires a much wider bandwidth. However, since the conventional disturbance observer attains the acceleration response by the second‐order derivative of position response, the bandwidth is limited because of the derivative noise. This paper proposes a novel structure of a disturbance observer. The proposed disturbance observer uses an acceleration sensor for enlargement of bandwidth. Generally, the bandwidth of an acceleration sensor is from 1 Hz to more than 1 kHz. To cover the DC range, the conventional position sensor‐based disturbance observer is integrated. Thus, the performance of the proposed multisensor based disturbance observer (MSDO) is superior to the conventional one. The MSDO is applied to position control (infinity stiffness) and force control (zero stiffness). The experimental results show the viability of the method proposed. © 2006 Institute of Electrical Engineers of Japan. Published by John Wiley &amp; Sons, Inc.
Threonine-4 of the budding yeast RNAP II CTD couples transcription with Htz1-mediated chromatin remodeling (2014)

The C-terminal domain (CTD) of the largest subunit of RNA polymerase II (RNAP II) consists of repeated YSPTSPS heptapeptides and connects transcription with cotranscriptional events. Threonine-4 (Thr4) of the CTD repeats has been shown to function in histone mRNA 3'-end processing in chicken cells and in transcriptional elongation in human cells. Here, we demonstrate that, in budding yeast, Thr4, although dispensable for growth in rich media, is essential in phosphate-depleted or galactose-containing media. Thr4 is required to maintain repression of phosphate-regulated (PHO) genes under normal growth conditions and for full induction of PHO5 and the galactose-induced GAL1 and GAL7 genes. We identify genetic links between Thr4 and the histone variant Htz1 and show that Thr4, as well as the Ino80 chromatin remodeler, is required for activation-associated eviction of Htz1 specifically from promoters of the Thr4-dependent genes. Our study uncovers a connection between transcription and chromatin remodeling linked by Thr4 of the CTD.
ChemInform Abstract: Hypoxic Radiosensitizers: Substituted Styryl Derivatives. (1995)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Cardiac Gene Transfer of Short Hairpin RNA Directed Against Phospholamban Effectively Knocks Down Gene Expression but Causes Cellular Toxicity in Canines (2011)

Derangements in calcium cycling have been described in failing hearts, and preclinical studies have suggested that therapies aimed at correcting this defect can lead to improvements in cardiac function and survival. One strategy to improve calcium cycling would be to inhibit phospholamban (PLB), the negative regulator of SERCA2a that is upregulated in failing hearts. The goal of this study was to evaluate the safety and efficacy of using adeno-associated virus (AAV)-mediated cardiac gene transfer of short hairpin RNA (shRNA) to knock down expression of PLB. Six dogs were treated with self-complementary AAV serotype 6 (scAAV6) expressing shRNA against PLB. Three control dogs were treated with empty AAV6 capsid, and two control dogs were treated with scAAV6 expressing dominant negative PLB. Vector was delivered via a percutaneously inserted cardiac injection catheter. PLB mRNA and protein expression were analyzed in three of six shRNA dogs between days 16 and 26. The other three shRNA dogs and five control dogs were monitored long-term to assess cardiac safety. PLB mRNA was reduced 16-fold, and PLB protein was reduced 5-fold, with treatment. Serum troponin elevation and depressed cardiac function were observed in the shRNA group only at 4 weeks. An enzyme-linked immunospot assay failed to detect any T cells reactive to AAV6 capsid in peripheral blood mononuclear cells, heart, or spleen. Microarray analysis revealed alterations in cardiac expression of several microRNAs with shRNA treatment. AAV6-mediated cardiac gene transfer of shRNA effectively knocks down PLB expression but is associated with severe cardiac toxicity. Toxicity may result from dysregulation of endogenous microRNA pathways.