gebhart/patrepeval huggingface dataset:
Insights into the in Vitro and in Vivo SAR of Abscisic Acid – Exploring Unprecedented Variations of the Side Chain via Cross‐Coupling‐Mediated Syntheses (2018)

Novel analogues of the plant hormone abscisic acid (ABA) were designed and prepared to explore the impact that modifications of its terpenoid side chain have on receptor affinity and in vivo efficacy against drought stress in selected crops. Their efficient and versatile synthesis proceeded via Stille or Sonogashira couplings, shortening the synthetic route significantly. In line with molecular modelling and X‐ray crystallography studies novel ABA‐derivatives with small alkyl, cycloalkyl or haloalkyl substituents showed strong effects in vitro and in vivo against drought stress in crops, particularly canola and wheat.
Cross-Linked Microparticles as Carriers for the Delivery of Plasmid DNA for Vaccine Development (2004)

Plasmid DNA was directly encapsulated into biocompatible polymer microparticles via radical polymerization in an inverse emulsion system. Acrylamide-based microspheres 0.2-1 microm in diameter were prepared using an acid-cleavable difunctional monomer. Retention of the DNA payload at physiological pH with complete release under acidic conditions at lysosomal pH was demonstrated. By trapping the plasmid DNA within the cross-linked microparticle, enzymatic degradation was prevented when exposed to serum nucleases. For vaccine development, these delivery vehicles were also investigated for their ability to generate immune responses when delivered to phagocytic cells of the immune system. Encapsulated plasmid DNA demonstrated immunostimulatory activity in macrophages, leading to cytokine secretion of IL-6 with a response approximately 40-fold higher than that achieved with DNA alone.
Estimation of the perceived level of reverberation using non-intrusive single-channel variance of decay rates (2017)

The increasing processing power of hearing aids and mobile devices has led to the potential for incorporation of dereverberation algorithms to improve speech quality for the listener. Assessing the effectiveness of deverberation algorithms using subjective listening tests is extremely time consuming and depends on averaging out listener variations over a large number of subjects. Also, most existing instrumental measures are intrusive and require knowledge of the original signal which precludes many practical applications. In this paper we show that the proposed non-intrusive single-channel algorithm is a predictor of the perceived level of reverberation that correlates well with subjective listening test results, outperforming many existing intrusive and non-intrusive measures. The algorithm requires only a single training step and has a very low computational complexity making it suitable for hearing aids and mobile telephone applications. The source code has been made freely available.
Quantum‐Dot‐Encoded Microbeads for Multiplexed Genetic Detection of Non‐amplified DNA Samples (2010)

Barcoding technologies have become the basis for a new generation of molecular diagnostic platforms for measuring biomarkers in a high-throughput, rapid, and sensitive manner. Thus far, researchers have mainly focused on preparing different types of barcodes but, in order to use them optimally in genomic- and proteomic-based applications, there is a need to understand the effect of barcode and assay parameters on their performance. Herein, quantum-dot barcodes are systematically characterized for the detection of non-amplified DNA sequences. The effect of capture probes, reporter probes, and target DNA sequence lengths are studied, as well as the effect of the amount of noncomplementary sequences on the hybridization kinetics and efficiency. From DNA denaturation to signal detection, quantum-dot-barcode assays require less than one hour to detect a target DNA sequence with a linear dynamic range of 0.02-100 fmol. Three optically distinct quantum-dot barcodes are used to demonstrate the multiplexing capability of these barcodes for genomic detection. These results suggest that quantum-dot barcodes are an excellent platform for multiplex, rapid, and sensitive genetic detection.
State-space modelling of slow-memory effects based on multisine vector measurements (2005)

Non-linear microwave devices and circuits often exhibit slow-memory effects. When subjected to two-tone, or more general multisine excitations, the characteristics of these devices and circuits depend on the offset frequency between the tones. Since modulated excitations are an integral part of telecommunication systems, models aimed for circuit and system design should be able to accurately represent slow-memory behaviour. In this work, we develop a modelling procedure based on the state-space modelling approach to accurately incorporate these slow-memory effects. The technique is experimentally demonstrated on a high electron mobility transistor (HEMT).
Ethylene/1‐hexene copolymerization with tetramethyldisiloxane‐bridged bis(indenyl) metallocenes (2004)

Abstract Ethylene/1‐hexene copolymerizations with disiloxane‐bridged metallocenes, rac ‐ and meso ‐1,1,3,3‐tetramethyldisiloxanediyl‐bis(1‐indenyl)zirconium dichloride ( rac ‐ 1 , meso ‐ 1 ) activated by modified methylaluminoxane were performed to investigate the influence of conformational dynamics on comonomer selectivity. Although 1 H NOESY (nuclear Overhauser and exchange spectroscopy) analysis indicated that the most stable conformation for the meso isomer in solution was that in which both indenes project over the metal coordination site, this isomer showed higher 1‐hexene selectivity in copolymerization (r e = 140 ± 30, r h = 0.024 ± 0.004) than the rac isomer with only one indene over the coordination site (r e = 240 ± 20, r h = 0.005 ± 0.001). The meso isomer showed high 1‐hexene selectivity, a high product of reactivity ratios (r e r h = 3.3 ± 0.5) and produced copolymers that could be separated into fractions with different ethylene content suggesting that the active species exhibited multisite behavior and populated conformations with different comonomer selectivities during the copolymerization. © 2004 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 42: 3323–3331, 2004
Lie Bodies: A Manifold Representation of 3D Human Shape. Supplemental Material (2012)

This technical report is complementary to [1] and contains proofs, formulas and additional plots. It is identical to the supplemental material submitted to European Conference on Computer Vision (ECCV 2012) on March 2012. References [1] Freifeld, O., Black, M.J.: Lie Bodies: A Manifold Representation of 3D Human Shape. European Conference on Computer Vision (2012) Lie Bodies: A Manifold Representation of 3D Human Shape Supplemental Material Oren Freifeld Division of Applied Mathematics, Brown University Michael J. Black Max Planck Institute for Intelligent Systems
T cell activation by conagenin in mice. (1993)

Conagenin (CNG), a low molecular immunomodulator, enhanced incorporation of [3H]thymidine into T cells activated by concanavalin A but did not to non-activated T cells. The culture supernatants of activated T cells treated with CNG enhanced incorporation of [3H]thymidine into cytokine dependent cell lines, CTLL-2 and IC-2 cells. This indicates that CNG exclusively acts on activated T cells and stimulates them to promote DNA synthesis and to produce lymphokines, which may include T cell growth factors and hematopoietic growth factors. These activities were also observed with T cells taken from mice given CNG.
Searches for the Zγ decay mode of the Higgs boson and for new high-mass resonances in pp collisions at s = 13 $$ \sqrt{s}=13 $$ TeV with the ATLAS detector (2017)

This article presents searches for the Z decay of the Higgs boson and for narrow high-mass resonances decaying to Z, exploiting Z boson decays to pairs of electrons or muons. The data analysis uses 36.1 fb -1 of pp collisions at s = 13 recorded by the ATLAS detector at the CERN Large Hadron Collider. The data are found to be consistent with the expected Standard Model background. The observed (expected -assuming Standard Model pp H Z production and decay) upper limit on the production cross section times the branching ratio for pp H Z is 6.6. (5.2) times the Standard Model prediction at the 95% confidence level for a Higgs boson mass of 125.09 GeV. In addition, upper limits are set on the production cross section times the branching ratio as a function of the mass of a narrow resonance between 250 GeV and 2.4 TeV, assuming spin-0 resonances produced via gluon-gluon fusion, and spin-2 resonances produced via gluongluon or quark-antiquark initial states. For high-mass spin-0 resonances, the observed (expected) limits vary between 88 fb (61 fb) and 2.8 fb (2.7 fb) for the mass range from 250 GeV to 2.4 TeV at the 95% confidence level.
Variability in the Geometry of RNA Double Helices Generated from Dinucleoside Building-Blocks (1985)

A graphical method is presented for the generation of helical parameters from single-crystal structures of RNA nucleic acid fragments that are minimally dinucleosides. The method is compared with other published procedures, for a number of text examples. The RNA double helices generated from three different salts of the dinucleoside monophosphate GpC are examined in relation to the variations in helix morphology that are produced. It is shown that small differences between these GpC salts can be amplified to very distinct helix characteristics.
4-Trifloxy-9-SEM-β-Carboline:  Preparation and Synthetic Utility (1999)

ADVERTISEMENT RETURN TO ISSUEPREVNote4-Trifloxy-9-SEM-β-Carboline: Preparation and Synthetic UtilityCarl A. Busacca, Magnus C. Eriksson, Yong Dong, Anthony S. Prokopowicz, Annette M. Salvagno, and Matt A. TschantzView Author Information Departments of Chemical Development and Medicinal Chemistry, Boehringer Ingelheim Pharmaceuticals, Inc., 900 Ridgebury Road, Ridgefield, Connecticut 06877-0368 Cite this: J. Org. Chem. 1999, 64, 12, 4564–4568Publication Date (Web):May 26, 1999Publication History Received19 February 1999Published online26 May 1999Published inissue 1 June 1999https://pubs.acs.org/doi/10.1021/jo990316thttps://doi.org/10.1021/jo990316tbrief-reportACS PublicationsCopyright © 1999 American Chemical SocietyRequest reuse permissionsArticle Views788Altmetric-Citations16LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Adducts,Ligands,Oxidation,Palladium,Reagents Get e-Alerts
Epigenetic inactivation of the CHFR gene in cervical cancer contributes to sensitivity to taxanes (2007)

A relationship between inactivation of mitotic checkpoint genes and sensitivity of cancer cells to anticancer agents has been reported. We investigated the effect of epigenetic inactivation by aberrant hypermethylation of the mitotic checkpoint gene CHFR (checkpoint with forkhead and ring finger) on the sensitivity of cervical cancer cells to taxanes. Methylation-specific PCR (MSP) of cervical smears showed aberrant methylation of CHFR in 12.3% (2/14) of adenocarcinoma specimens. In contrast, aberrant DNA methylation was not detected in normal cervical cells or squamous cell carcinoma cells. Aberrant methylation of CHFR was also analyzed in 6 human cervical carcinoma-derived cell lines and was observed in SKG-IIIb and HeLa cells. These cell lines showed high sensitivity to taxanes, but became taxane-resistant upon treatment with 5-azacytidine. Furthermore, suppression of CHFR expression in siRNA-transfected SKG-IIIa cells caused increased sensitivity to taxanes. In conclusion, aberrant methylation of the CHFR gene may be useful as a molecular marker for selection of therapy for patients with cervical adenocarcinoma with a poor prognosis, and may also suggest a new therapeutic strategy of targeting CHFR in cervical cancer. To our knowledge, this study is the first to examine epigenetic inactivation by aberrant hypermethylation of CHFR in cervical cancer.
Search for Physics beyond the Standard Model in Events with Overlapping Photons and Jets (2019)

Results are reported from a search for new particles that decay into a photon and two gluons, in events with jets. Novel jet substructure techniques are developed that allow photons to be identified in an environment densely populated with hadrons. The analyzed proton-proton collision data were collected by the CMS experiment at the LHC, in 2016 at ffiffi ffi s p 13 TeV, and correspond to an integrated luminosity of 35.9 fb -1 . The spectra of total transverse hadronic energy of candidate events are examined for deviations from the standard model predictions. No statistically significant excess is observed over the expected background. The first cross section limits on new physics processes resulting in such events are set. The results are interpreted as upper limits on the rate of gluino pair production, utilizing a simplified stealth supersymmetry model. The excluded gluino masses extend up to 1.7 TeV, for a neutralino mass of 200 GeV and exceed previous mass constraints set by analyses targeting events with isolated photons.
Optogenetics-based localization of talin to the plasma membrane promotes activation of β3 integrins (2021)

Interaction of talin with the cytoplasmic tails of integrin β triggers integrin activation, leading to an increase of integrin affinity/avidity for extracellular ligands. In talin KO mice, loss of talin interaction with platelet integrin αIIbβ3 causes a severe hemostatic defect, and loss of talin interaction with endothelial cell integrin αVβ3 affects angiogenesis. In normal cells, talin is autoinhibited and localized in the cytoplasm. Here, we used an optogenetic platform to assess whether recruitment of full-length talin to the plasma membrane was sufficient to induce integrin activation. A dimerization module (Arabidopsis cryptochrome 2 fused to the N terminus of talin; N-terminal of cryptochrome-interacting basic helix-loop-helix domain ended with a CAAX box protein [C: cysteine; A: aliphatic amino acid; X: any C-terminal amino acid]) responsive to 450 nm (blue) light was inserted into Chinese hamster ovary cells and endothelial cells also expressing αIIbβ3 or αVβ3, respectively. Thus, exposure of the cells to blue light caused a rapid and reversible recruitment of Arabidopsis cryptochrome 2-talin to the N-terminal of cryptochrome-interacting basic helix-loop-helix domain ended with a CAAX box protein [C: cysteine; A: aliphatic amino acid; X: any C-terminal amino acid]-decorated plasma membrane. This resulted in β3 integrin activation in both cell types, as well as increasing migration of the endothelial cells. However, membrane recruitment of talin was not sufficient for integrin activation, as membrane-associated Ras-related protein 1 (Rap1)-GTP was also required. Moreover, talin mutations that interfered with its direct binding to Rap1 abrogated β3 integrin activation. Altogether, these results define a role for the plasma membrane recruitment of talin in β3 integrin activation, and they suggest a nuanced sequence of events thereafter involving Rap1-GTP.
Frequency-modulation spectroscopy for trace species detection: theory and comparison among experimental methods: errata (1992)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text Joel A. Silver, "Frequency-modulation spectroscopy for trace species detection: theory and comparison among experimental methods: errata," Appl. Opt. 31, 4927-4927 (1992) Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
The Development of Acoustic Emission for Leak Detection and Location in Liquid-Filled, Buried Pipelines (1999)

Acoustic Emission leak detection and location for liquid-filled, buried pipelines was studied using various leak sources and laboratory reference standards.These standards provided significant data that have contributed to the development of test procedures for generating two-phase flow and a better understanding of the leak mechanism. Results are discussed for leak signal enhancement due to two-phase flow, leak orientation and the presence of backfill surrounding the pipe. Field studies were performed on buried pipes to demonstrate that small leaks (less than 0.1 gallons/hour or 0.1 mL/second) can be detected under the right test conditions and that the location of these small leaks can be determined using a variety of location methods. Among the different location methods examined was a location technique referred to as “tuned” linear location. In addition, a modification to the conventional Signal Difference location technique is discussed.
Modeling a Crowdsourced Definition of Molecular Complexity (2014)

This paper brings together the concepts of molecular complexity and crowdsourcing. An exercise was done at Merck where 386 chemists voted on the molecular complexity (on a scale of 1-5) of 2681 molecules taken from various sources: public, licensed, and in-house. The meanComplexity of a molecule is the average over all votes for that molecule. As long as enough votes are cast per molecule, we find meanComplexity is quite easy to model with QSAR methods using only a handful of physical descriptors (e.g., number of chiral centers, number of unique topological torsions, a Wiener index, etc.). The high level of self-consistency of the model (cross-validated R(2) ∼0.88) is remarkable given that our chemists do not agree with each other strongly about the complexity of any given molecule. Thus, the power of crowdsourcing is clearly demonstrated in this case. The meanComplexity appears to be correlated with at least one metric of synthetic complexity from the literature derived in a different way and is correlated with values of process mass intensity (PMI) from the literature and from in-house studies. Complexity can be used to differentiate between in-house programs and to follow a program over time.
A Small-Size High-Rejection LTCC Diplexer for WLAN Applications Based on a New Dual-Band Bandpass Filter. (2007)

This paper presents a new very small low temperature co-fired ceramic (LTCC) diplexer based on a dual-band bandpass filter for wireless LAN (IEEE 802.11 a/b/g) application. The diplexer and the dual-band filter design have been realized and demonstrated in this article. Measurement results agree quite well with simulation. The new type of such diplexer significantly reduces the number of components and the size of dual-band WLAN frontend modules.
Tailoring the Cation Lattice for Chloride Lithium‐Ion Conductors (2020)

Abstract All‐solid‐state Li‐ion batteries require Li‐ion conductors as solid electrolytes (SEs). Li‐containing halides are emerging as a promising class of lithium‐ion conductors with good electrochemical stability and other properties needed for SEs in all‐solid‐state batteries. Compared to oxides and sulfides, Li‐ion diffusion mechanisms in Li‐containing halides are less well understood, in particular regarding the effects of Li content and cation sublattices, which can be tailored for improving Li‐ion conduction. Using first‐principles computation, a systematic study is performed on the Li‐ion conduction of known Li‐containing chlorides with close‐packed anion frameworks and a wide range of their doped compounds. A dozen potential chloride Li‐ion conductors are predicted with increased Li‐ion conductivities, and it is revealed that the Li‐ion migration is greatly impacted by the cation configuration and concentrations. By analyzing a large set of materials data, it is proposed that low Li content, low cation concentration, and sparse cation distribution increase Li‐ion conduction in chlorides, and these principles are demonstrated in designing new chloride Li‐ion conductors. This study provides insights into the effects of the cation sublattice on Li‐ion diffusion, highlights potential chloride Li superionic conductors, and proposes design principles to further develop halide Li‐ion conductors.
Analysis and design of zero-voltage-switching class-E converter (2002)

In this paper, a new class-E DC-to-DC converter with half-wave controlled current rectifier is proposed. This is a high order multiple resonant converter whose circuit modes of operation have different resonant frequencies. The basic converter operation, detailed steady state analysis, steady state control characteristics and the peak values of inductance current and capacitance voltage are given. Based on the derived control characteristic curves, a design example along with a design procedure is demonstrated. The results are verified by PSPICE simulation. It is shown that this topology retains all the advantages of the conventional class-E converter and still provides zero-voltage-switching and results in low component stress values.