gebhart/patrepeval huggingface dataset:
Altered astrocytic response to activation in SOD1G93A mice and its implications on amyotrophic lateral sclerosis pathogenesis (2012)

Amyotrophic Lateral Sclerosis (ALS) is a fatal, rapidly progressive, neurodegenerative disease caused by motor neuron degeneration. Despite extensive efforts, the underlying cause of ALS and the path of neurodegeneration remain elusive. Astrocyte activation occurs in response to central nervous system (CNS) insult and is considered a double edged sword in many pathological conditions. We propose that reduced glutamatergic and trophic response of astrocytes to activation may, over time, lead to accumulative CNS damage, thus facilitating neurodegeneration. We found that astrocytes derived from the SOD1(G93A) ALS mouse model exhibit a reduced glutamatergic and trophic response to specific activations compared to their wild-type counterparts. Wild-type astrocytes exhibited a robust response when activated with lipopolysaccharide (LPS), G5 or treated with ceftriaxone in many parameters evaluated. These parameters include increased expression of GLT-1 and GLAST the two major astrocytic glutamate transporters, accompanied by a marked increase in the astrocytic glutamate clearance and up-regulation of neurtrophic factor expression. However, not only do un-treated SOD1(G93A) astrocytes take up glutamate less efficiently, but in response to activation they show no further increase in any of the glutamatergic parameters evaluated. Furthermore, activation of wild-type astrocytes, but not SOD1(G93A) astrocytes, improved their ability to protect the motor neuron cell line NSC-34 from glutamate induced excitotoxicity. Our data indicates that altered astrocyte activation may well be pivotal to the pathogenesis of ALS.
Experimental investigation and numerical simulation of a slab waveguide CO2laser with rf pumping (1996)

Experimental and theoretical investigations were made of the active-medium gain and of the parameters of the radiation emitted by an rf-pumped slab CO2 laser. The average output power was 160 W and the efficiency was 10%. Near-diffraction-limited divergence was achieved. In the pulse-periodic regime, the pulse power was twice the average power in the cw regime. When external correcting optics was used, the total divergence of the laser radiation was 2–3 mrad at 0.8 of the total power. A suite of programs for numerical simulation of the physical processes in an rf-pumped slab CO2 laser with diffusion cooling was developed. The experimental and theoretical results were compared.
A coordination-free distributed algorithm for simple assignment problems using randomized actions (2011)

In this paper, we present a distributed algorithm for simple assignment problems in multi-agent systems without the presence of a central or a local coordinator. In a simple assignment problem, a unique task is to be assigned to each agent assuming that the cost of each assignment is identical. We assume that the communication among the agents is restricted over a sparse network, i.e., no agent can communicate to all of the other agents in the network. We propose a coordination-free, memoryless, distributed algorithm that is based on inter-agent information exchange via a gossip (type) communication protocol. Each agent randomly picks a task and then either swaps its task with a randomly chosen neighbor from its neighborhood (if they have different tasks) or one of them randomly picks a new task (if they have the same task). We first consider the case when the agents have no memory such that they do not have information regarding their task and neighbor history. We then present a modification when the agents possess memory. Finally, we show that both schemes converge to the set of acceptable configurations.
Graphene Nanosheets as Negative Electrode for Lithium Ion Battery (2012)

Abstract not Available.
Economic Impact of Multicore Erbium-Ytterbium doped Fiber Amplifier in Long-Haul Optical Transport Networks (2018)

We investigate for the first time the technoeconomic impact of 12-core Erbium-Ytterbium doped fiber amplifiers used in optical transport networks. We evaluate the CAPEX gain involved by their introduction as line and ROADM amplifiers and the energy consumption reduction.
Identification of ADAR1 adenosine deaminase dependency in a subset of cancer cells (2018)

Systematic exploration of cancer cell vulnerabilities can inform the development of novel cancer therapeutics. Here, through analysis of genome-scale loss-of-function datasets, we identify adenosine deaminase acting on RNA (ADAR or ADAR1) as an essential gene for the survival of a subset of cancer cell lines. ADAR1-dependent cell lines display increased expression of interferon-stimulated genes. Activation of type I interferon signaling in the context of ADAR1 deficiency can induce cell lethality in non-ADAR1-dependent cell lines. ADAR deletion causes activation of the double-stranded RNA sensor, protein kinase R (PKR). Disruption of PKR signaling, through inactivation of PKR or overexpression of either a wildtype or catalytically inactive mutant version of the p150 isoform of ADAR1, partially rescues cell lethality after ADAR1 loss, suggesting that both catalytic and non-enzymatic functions of ADAR1 may contribute to preventing PKR-mediated cell lethality. Together, these data nominate ADAR1 as a potential therapeutic target in a subset of cancers.
Strain Improvement of Rhodotorula graminis for Production of a Novel l -Phenylalanine Ammonia-Lyase (1988)

l-Phenylalanine ammonia-lyase (PAL; EC 4.3.1.5) from Rhodotorula rubra has been used in the commercial manufacture of l-phenylalanine from trans-cinnamic acid. In this study, R. graminis PAL was investigated. Mutant strain GX6000 was isolated after ethyl methanesulfonate mutagenesis of wild-type R. graminis GX5007 by selecting for resistance to phenylpropiolic acid, an analog of trans-cinnamic acid. Mutant strain GX6000 produced inducible PAL at levels four- to fivefold higher than had wild-type R. graminis. Furthermore, this strain had several other physiological traits that make it more commercially useful than R. rubra. For example, during fermentation, the PAL half-life was three- to fivefold longer, PAL specific activity was six to seven times higher, and PAL synthesis was significantly less inhibited by temperatures above 30 degrees C. Induction of PAL in strain GX6000 appeared to be less tightly regulated; l-leucine acted synergistically with l-phenylalanine, the physiological inducer, to increase the PAL specific activity and titer to 165 U/g (dry weight) and 3,000 U/liter, respectively, a 40% increase over the effect of l-phenylalanine alone. Strain GX6000 PAL showed significantly greater stability in bioreactors for the synthesis of l-phenylalanine, a finding that is consistent with the stability properties observed during fermentation.
Three-dimensional model of a selective theophylline-binding RNA molecule (1996)

A three-dimensional (3D) model for an RNA molecule that selectively binds theophylline but not caffeine is proposed. This RNA, which was found using SELEX (Jenison et al., 1994), is 10,000 times more specific for theophylline (Kn = 320 nM) than for caffeine (KD = 3.5 mM), although the two ligands are identical except for a methyl group substituted at N7 (present only in caffeine). The binding affinity for ten xanthine-based ligands was used to derive a comparative molecular field analysis model (R2 = 0.93 for three components, with cross-validated R2 of 0.73), using the SYBYL and GOLPE programs. A pharmacophoric map was generated to locate steric and electrostatic interactions between theophylline and the RNA binding site. This information was used to identify putative functional groups of the binding pocket and to generate distance constraints. On the basis of a model for the secondary structure (Jenison et al., 1994), the 3D structure of this RNA was then generated using the following method: each helical region of the RNA molecule was treated as a rigid body; single-stranded loops with specific end-to-end distances were generated. The structures of RNA-xanthine complexes were studied using a modified Monte Carlo algorithm. The detailed structure of an RNA-ligand complex model, as well as possible explanations for the theophylline selectivity are discussed.
Friction stir welding of aluminium lap joint by tool without probe (2010)

A friction stir welding process, with a rotating tool without a probe, was employed and applied to a lap joint of aluminium plate. The thickness of the aluminium plates was 0.5 mm. New tool shapes were developed. The tops of the tool were dome shaped. In this process, the rotating tool was plunged into the aluminium plate. The tool-rotating axis was vertical to the specimen surface, and then moved in the welding direction at a speed of 20 mm/s. Tool rotation speed was 18,000 rpm. At tool plunge depths of 0.1 mm or over, it was possible to weld the two plates. At tool plunge depth of 0.1 mm, its joint was fractured at the weld interface. At tool plunge depth of 0.2 mm or over, the joints were fractured at the stir zone of the upper plate or the heat affected zone of the lower plate. Based on observation of the hardness profiles and the thickness change of the weld area, controlling factors of the joint strength are discussed.
Nebulizer (2016)

This Glossary of Terms Related to Pharmaceutics is needed by practitioners in the field of pharmaceutics as this field fulfills an important and crucial role, different from the roles of other scientific disciplines involved in the drugmaking process. The glossary contains 168 definitions used in pharmaceutics. These are related to various aspects of this discipline, such as: (1) physicochemical characterization of pharmaceutical preparations and the active ingredients they contain; (2) unit operations used in the practice of pharmaceutics; (3) terms related to the various dosage forms; (4) terms related to the various modes and routes of drug delivery; (5) terms used in pharmacokinetics and biopharmaceutics in general; and additional miscellaneous terms. The field of pharmaceutics itself is of a multidisciplinary nature as its practitioners come from a variety of disciplines, such as chemistry or various biological sciences, thus a glossary containing authoritative definitions would be useful for them. The terms used in pharmaceutics are rarely covered by existing glossaries, and in the cases they are, their definitions are often inappropriate for the field of pharmaceutics and require new or modified definitions to better fit the new context.
The Analysis of Nano-Patterned Sapphire Substrates-Induced Compressive Strain to Enhance Quantum-Confined Stark Effect of InGaN-Based Light-Emitting Diodes (2013)

This paper demonstrates that the quantum-confined stark effect of InGaN-based light-emitting diodes can be enhanced by the means of using the hexagonal nano-post patterned sapphire substrates based on the increase of the post-duty cycle.
A Sub-mW 18-MHz MEMS Oscillator Based on a 98-dBΩ Adjustable Bandwidth Transimpedance Amplifier and a Lamé-Mode Resonator (2019)

This paper presents a microelectromechanical system (MEMS)-based oscillator based on a Lamé-mode capacitive micromachined resonator and a fully differential high-gain transimpedance amplifier (TIA). The proposed TIA is designed using TSMC 65 nm CMOS technology and consumes only 0.9 mA from a 1-V supply. The measured mid-band transimpedance gain is 98 dB Ω and the TIA features an adjustable bandwidth with a maximum bandwidth of 142 MHz for a parasitic capacitance C P of 4 pF. The measured input-referred current noise of the TIA at mid-band is below 15 pA/ Hz . The TIA is connected to a Lamé-mode resonator, and the oscillator performance in terms of phase noise and frequency stability is presented. The measured phase noise under vacuum is -120 dBc/Hz at a 1-kHz offset, while the phase noise floor reaches -127 dBc/Hz. The measured short-term stability of the MEMS-based oscillator is ±0.25 ppm.
A nonlinear model of the phasic dynamics of muscle activation (1990)

This paper presents a phasic excitation-activation (PEXA) model of the process of motoneuron excitation and the resultant activation and force development of a motor unit. The model input is an amount of depolarizing current (as when injected with an intracellular electrode) and the model output is muscle force. The model includes dynamics and nonlinearities similar to phenomena discovered experimentally by others: the firing rate response of motoneurons to steps of depolarizing current and the "catch-like enhancement" of force produced by overlapping motor neuron action potentials. The parameter values used in this model are derived from experimentally measured data and expressed in physical units, and model predictions extend to published data beyond those used in generating the model parameter values.
Noncoherent Detection for Physical-Layer Network Coding (2018)

This paper investigates noncoherent detection in a two-way relay channel operated with physical layer network coding (PNC), assuming FSK modulation and short-packet transmissions. For noncoherent detection, the detector has access to the magnitude but not the phase of the received signal. For conventional communication in which a receiver receives the signal from a transmitter only, the phase does not affect the magnitude, hence the performance of the noncoherent detector is independent of the phase. PNC, however, is a multiuser system in which a receiver receives signals from multiple transmitters simultaneously. The relative phase of the signals from different transmitters affects the received signal magnitude through constructive-destructive interference. In particular, for good performance, the noncoherent detector in PNC must take into account the influence of the relative phase on the signal magnitude. Building on this observation, this paper delves into the fundamentals of PNC noncoherent detector design. To avoid excessive overhead, we do away from preambles. We show how the relative phase can be deduced directly from the magnitudes of the received data symbols. Numerical results show that our detector performs nearly as well as a "fictitious" optimal detector that has perfect knowledge of the channel gains and relative phase.
Mitochondrial DNA Sequence Heterogeneity of Single CD34+ Cells After Nonmyeloablative Allogeneic Stem Cell Transplantation (2007)

We applied a single-cell method to detect mitochondrial DNA (mtDNA) mutations to evaluate the reconstitution of hematopoietic stem cells (HSCs) and committed progenitor cells after nonmyeloablative allogeneic stem cell transplantation in humans. In a total of 1,958 single CD34(+) cells from six human leukocyte antigen-matched sibling donor and recipient pairs, individual CD34(+) clones were recognized based on the observed donor- or recipient-specific mtDNA sequence somatic alteration. There was no overall reduction of mtDNA heterogeneity among CD34(+) cells from the recipient after transplantation. Samples collected from two donors over time showed the persistence of certain CD34(+) clones marked by specific mutations. Our results demonstrate the feasibility of distinguishing donor and recipient individual CD34(+) clones based on mtDNA mutations during engraftment. HSCs were not limited in number, and similar mtDNA heterogeneity levels suggested representation of the total stem cell compartment during rapid hematopoietic reconstitution in the recipient. Disclosure of potential conflicts of interest is found at the end of this article.
Ag(I)-Catalyzed C–H Carboxylation of Thiophene Derivatives (2021)

CO2 utilization is an attractive aspect as it allows the direct conversion of CO2 into valuable chemicals. In this regard, direct incorporation of CO2 into the C–H bond of heteroaromatic compounds is important due to the ubiquitous structural motifs of the heteroaromatic carboxylic acids. Herein, we report the Ag-catalyzed C–H carboxylation of thiophene derivatives. This new catalytic system involving a phosphine ligand and lithium tert-butoxide enables the direct carboxylation of thiophenes under mild reaction conditions. Experimental studies revealed that the use of tert-butyl alkoxide is critical for the exergonic formation of an arylsilver intermediate, and the results were further supported by density functional theory calculations.
DDAVP in the Urological Management of the Difficult Neurogenic Bladder in Spinal Cord Injury: Preliminary Report (1994)

The purpose of this study is to determine the efficacy of desmopressin (DDAVP), a synthetic analogue of antidiuretic hormone, as an alternative therapy in the management of spinal cord injured (SCI) patients with neurogenic bladder dysfunction unresponsive to conventional therapy. Seven SCI patients (three men and four women) were treated with DDAVP after urodynamic evaluation. Despite treatment with anticholinergic agents, urodynamic evaluation demonstrated uninhibited detrusor contractions exceeding 30 cm H2O pressure at less than 300 ml cystometric capacity in all seven patients. Three patients had been managed with intermittent self-catheterization, but had socially unacceptable short intervals between catheterizations. Two women with incomplete injury were afflicted with significant nocturia (> 3 episodes/night). The remaining two patients managed with intermittent self-catheterization were troubled with nocturnal enuresis. The patients received 10 micrograms intranasal DDAVP once every 24 hours. Prior to DDAVP administration, the four patients who used DDAVP nightly experienced a median of four episodes of nocturia. After one month of DDAVP treatment, two patients had only one episode of nocturia per night and in the other two patients, nocturnal enuresis was completely eliminated. Three patients used daytime DDAVP administration at work to avoid frequent catheterization. The median period between bladder catheterizations increased from 2.5 hours before DDAVP to 6 hours while using DDAVP. Symptomatic improvement persisted during the follow-up period of 6-20 months (mean = 12). Side effects were infrequent; only one patient complained of transient headaches. Neither hyponatremia nor serum electrolyte abnormalities occurred. Our preliminary results suggest that DDAVP is safe and effective in the symptomatic management of complicated neurogenic bladder dysfunction in selected SCI patients.
Shape memory alloy resetable spring lift for pedestrian protection (2008)

Pedestrian protection has become an increasingly important aspect of automotive safety with new regulations taking effect around the world. Because it is increasingly difficult to meet these new regulations with traditional passive approaches, active lifts are being explored that increase the "crush zone" between the hood and rigid under-hood components as a means of mitigating the consequences of an impact with a non-occupant. Active lifts, however, are technically challenging because of the simultaneously high forces, stroke and quick timing resulting in most of the current devices being single use. This paper introduces the SMArt (Shape Memory Alloy ReseTable) Spring Lift, an automatically resetable and fully reusable device, which couples conventional standard compression springs to store the energy required for a hood lift, with Shape Memory Alloys actuators to achieve both an ultra high speed release of the spring and automatic reset of the system for multiple uses. Each of the four SMArt Device subsystems, lift, release, lower and reset/dissipate, are individually described. Two identical complete prototypes were fabricated and mounted at the rear corners of the hood, incorporated within a full-scale vehicle testbed at the SMARTT (Smart Material Advanced Research and Technology Transfer) lab at University of Michigan. Full operational cycle testing of a stationary vehicle in a laboratory setting confirms the ultrafast latch release, controlled lift profile, gravity lower to reposition the hood, and spring recompression via the ratchet engine successfully rearming the device for repeat cycles. While this is only a laboratory demonstration and extensive testing and development would be required for transition to a fielded product, this study does indicate that the SMArt Lift has promise as an alternative approach to pedestrian protection.
Ocular Findings Associated With a Rhodopsin Gene Codon 58 Transversion Mutation in Autosomal Dominant Retinitis Pigmentosa (1991)

Eight members of a family with autosomal dominant retinitis pigmentosa were found to have a cytosine-to-guanine (C-to-G) transversion mutation in the second nucleotide of codon 58 of the rhodopsin gene, causing a substitution of the amino acid arginine for threonine. Five of these individuals were examined clinically. There was a distinct phenotypic expression of the gene defect within this family that included a regional predilection for pigmentary changes in the inferior and inferonasal parts of the retina, as well as field impairment predominantly in the superior hemisphere. Characteristic electroretinographic recordings and psychophysical threshold profiles also helped to identify this phenotype that, on a relative basis, causes less severe photoreceptor cell functional impairment than often occurs in other subtypes of retinitis pigmentosa. This report documents the association of a clinically recognizable phenotype in a family with autosomal dominant retinitis pigmentosa and a specific gene defect at the molecular level.
Solutions, suspensions et émulsions destinées aux voies orale, parentérale et ophtalmique (2007)

International audience
Metabolism of NG-monomethyl-L-arginine (1983)

Rat kidney contains an enzyme which hydrolyzes NG-monomethyl-L-arginine to give rise to the formation of ornithine and N-methylurea. Confirmation of formation of these reaction products is carried out colorimetrically as well as radiochemically employing NG-monomethyl-L-[ornithine-14C(U)]arginine. This pattern of reaction products suggests that the enzyme responsible is an arginase type. However, the kidney enzyme is quite distinct from the hepatic arginase; commercial bovine hepatic arginase (L-arginine amidinohydrolase, EC 3.5.3.1) is completely inactive toward NG-monomethyl-L-arginine. Among various rat tissues examined, the hydrolytic activity is the highest in kidney, followed by the activity in liver and pancreas.