gebhart/patrepeval huggingface dataset:
HLA‐linked heat‐shock protein 70 (HSP70–2) gene polymorphism and celiac disease (1993)

The restriction enzyme Pstl showed a two-allele restriction fragment length polymorphism (RFLP) marker when DNA polymorphism of the gene G8, a novel major histocompatibility complex (MHC) class III gene, was screened. The gene G8 is located ca. 4 kb centromeric of the heat-shock protein 70 (HSP70) gene cluster. A more detailed mapping indicated that the polymorphic restriction site is actually located in the coding region of the adjacent HSP70-2 gene, where it has been earlier reported to cause a silent mutation. To estimate the frequency of this polymorphism in the normal population, 95 blood donors were analyzed: The gene frequency of the 8.5 kb (designated 'L') allele was 0.45 and that of the 8.65 kb ('U') allele 0.55. However, when 19 families with patients suffering from celiac disease were studied, the gene frequencies in the affected haplotypes (L = 0.76, U = 0.24) significantly deviated from those observed in the normal population and in the non-affected MHC haplotypes of these families (L = 0.48, U = 0.52). However, the association results from a strong association between the allele 'L' and the MHC haplotype HLA B8 DR3, a known suspectibility marker of celiac disease. Only one patient, in fact, was negative for the well-established class II haplotype markers DR3 or DR7. The data therefore confirm the crucial role of MHC class II in suspectibility to celiac disease, but due to a strong linkage disequilibrium within MHC the role of MHC class III genes in disease associations can not be ruled out.
Differential expression of cell surface proteins in human bone marrow mesenchymal stem cells cultured with or without basic fibroblast growth factor containing medium (2009)

Mesenchymal stem cells (MSCs) are multipotent cells, which have the capability to differentiate into various mesenchymal tissues such as bone, cartilage, fat, tendon, muscle, and marrow stroma. However, they lose the capability of multi-lineage differentiation after several passages. It is known that basic fibroblast growth factor (bFGF) increases growth rate, differentiation potential, and morphological changes of MSCs in vitro. In this report, we have used 2-DE coupled to MS to identify differentially expressed proteins at the cell membrane level in MSCs growing in bFGF containing medium. The cell surface proteins isolated by the biotin-avidin affinity column were separated by 2-DE in triplicate experiments. A total of 15 differentially expressed proteins were identified by quadrupole-time of flight tandem MS. Nine of the proteins were upregulated and six proteins were downregulated in the MSCs cultured with bFGF containing medium. The expression level of three actin-related proteins, F-actin-capping protein subunit alpha-1, actin-related protein 2/3 complex subunit 2, and myosin regulatory light chain 2, was confirmed by Western blot analysis. The results indicate that the expression levels of F-actin-capping protein subunit alpha-1, actin-related protein 2/3 complex subunit 2, and myosin regulatory light chain 2 are important in bFGF-induced morphological change of MSCs.
Supplementary Tables S1-8 from Functional Kinomics Identifies Candidate Therapeutic Targets in Head and Neck Cancer (2023)

&lt;p&gt;Tables S1-8:Table S1. Characteristics of the HNSCC cell lines Table S2. Characteristics of the MSCC cell lines with germline mutations in p53 pathway genes Table S3. Human kinome (713 kinases) Sigma Table S4. Murine kinome (572 kinases) Ambion Table S5. Interspecies kinome (508 kinases) Table S6. Primary validation siRNAs Table S7. Secondary validation siRNAs Table S8. Interspecies kinase targets selected for validation&lt;/p&gt;
Enantioselective Synthesis of Allylsilanes Bearing Tertiary and Quaternary Si‐Substituted Carbons through Cu‐Catalyzed Allylic Alkylations with Alkylzinc and Arylzinc Reagents (2007)

Alle Arten von Allylsilanen, darunter erstmals auch welche mit einem Si-gebundenen quartären Kohlenstoffatom, wurden effizient und hoch enantioselektiv mit den Titelreaktionen erhalten. Eine Vielzahl an Komplexen chiraler N-heterocyclischer Carbene kann zur Unterstützung eingesetzt werden. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2001/2007/z700841_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Results and evaluation of a first‐in‐human study of RG7342, an mGlu5 positive allosteric modulator, utilizing Bayesian adaptive methods (2017)

Single oral doses of RG7342 were generally tolerated up to 0.6 mg under fasting and 0.9 mg under fed conditions in healthy subjects. Bayesian adaptive methods describing the probability of DLEs were applied effectively to support dose escalation. MTDs (fasting, fed) were associated with a C<sub>max</sub> of 6.5 ng ml<sup>-1</sup> . The development of RG7342 was discontinued owing to the potential challenges associated with a long half-life in context of the observed adverse events.
Eosin Y: Homogeneous Photocatalytic In-Flow Reactions and Solid-Supported Catalysts for In-Batch Synthetic Transformations (2020)

In this paper, the most recent and significant applications of Eosin Y as an organo-photocatalyst will be discussed, focusing the attention on enabling technological aspects in homogeneous photochemical flow reactions, as well as on recent developments in solid-supported catalyst applications for batch synthetic transformations.
Si Substitution in Nanotubes and Graphene via Intermittent Vacancies (2019)

The properties of single-walled carbon nanotubes (SWCNTs) and graphene can be modified by the presence of covalently bound impurities. Although this can be achieved by introducing chemical additives during synthesis, that often hinders growth and leads to limited crystallite size and quality. Here, through the simultaneous formation of vacancies with low-energy argon plasma and the thermal activation of adatom diffusion by laser irradiation, silicon impurities are incorporated into the lattice of both materials. After an exposure of $\sim$1 ion/nm$^{2}$, we find Si substitution densities of 0.15 nm$^{-2}$ in graphene and 0.05 nm$^{-2}$ in nanotubes, as revealed by atomically resolved scanning transmission electron microscopy. In good agreement with predictions of Ar irradiation effects in SWCNTs, we find Si incorporated in both mono- and divacancies, with $\sim$2/3 being of the first type. Controlled inclusion of impurities in the quasi-1D and 2D carbon lattices may prove useful for applications such as gas sensing, and a similar approach might also be used to substitute other elements with migration barriers lower than that of carbon.
High-resolution deletion mapping of chromosome arm 1p in pancreatic cancer identifies a major consensus region at 1p35 (1999)

Chromosomal arm 1p has long been suspected, on the basis of loss of heterozygosity (LOH) and other data, to harbor a tumor suppressor gene important in pancreatic carcinomas and other tumors. We constructed a high-resolution map of LOH at I p in a panel of pancreatic adenocarcinomas. Using 44 markers, we identified LOH on I p in 49% of 43 cancers. Breakpoints in 1p were identified in 15 of the carcinomas and could be used to ascertain consensus patterns. We found a major consensus region of LOH at 1p35 between loci D1S233 and D1S247. This region participates in the majority of LOH events on 1 p in pancreatic cancer. These data provide a roadmap for further regional mapping, homozygous deletion searches, comparison to LOH patterns seen in other tumor types, and prioritization of studies using candidate genes.
Evidence against sink limitation by the sucrose‐to‐starch route in potato plants expressing fructosyltransferases (2011)

To investigate whether the route from sucrose to starch limits sink strength of potato tubers, we established an additional storage carbohydrate pool and analyzed allocation of imported assimilates to the different pools. Tuber specific expression of the fructan biosynthetic enzymes of globe artichoke resulted in accumulation of fructans to about 5% of the starch level, but did not increase tuber dry weight per plant. While partial repression of starch synthesis caused yield reduction in wild-type plants, it stimulated fructan accumulation, and yield losses were ameliorated in tubers expressing fructosyltransferases. However, a nearly complete block of the starch pathway by inhibition of sucrose synthase could not be compensated by the fructan pathway. Although fructan concentrations rose, yield reduction was even enhanced, probably because of a futile cycle of fructan synthesis and degradation by invertase, which is induced when sucrose synthase is knocked out. The data do not support a limitation of sink strength by enzyme activities of the starch pathway but point to an energy limitation of storage carbohydrate formation in potato tubers.
Far-infrared studies at intermediate magnetic fields with the neutral shallow donors in GaAs and InP of transitions not involving the ground state (1986)

More than thirty sharp lines are observed in the far-infrared photoconductivity from the neutral shallow donors in n-GaAs and InP at wavelengths longer than the longest wavelength that will excite electrons from the ground (1s) state of the donor. Twenty-four of the lines observed can be positively identified as corresponding to transitions given by p to q where p and q are the principal quantum numbers involved and are given by 2, 3 and 4. The n=2 to 2 lines involving the 2s state (2p - -2s, 2s-2p + , 2s-2p 0 ) are sufficiently sharp that central-cell structure arising from the chemical shifts of the individual donor species can be observed. The central-cell splitting is found to agree well with that expected from measurements of the splitting on the 1s state. The inter-excited-state transitions are observed under much less restricted conditions of sample compensation, electric field bias, optical excitation and temperature than the broader peaks corresponding to transitions from negatively charged donor-ion (D - ) states to the conduction band states.
Impact of composite dynamic load on the dynamic security region of power systems (2011)

Dynamic security region (DSR) is one of the direct methods focusing on the transient stability of power system. This paper explores the impact of composite dynamic load (CDL) characteristic on the DSR, which including the load model consisting of constant impedance load and induction motor load (ZM), the load model consisting of constant current load and induction motor load (IM) and the load model consisting of constant power load and induction motor load (PM). As the introduction of the composite dynamic load could lead to the differential-algebraic equation (DAE) structure for power system, this paper presents the algorithm for the DSR of power system with DAE structure, after the presentation of DSR algorithm for power system with ordinary differential equation (ODE) structure. It also presents the models for the composite dynamic load, i.e., ZM load, IM load and PM load. And then, based on the simulation results, the DSRs of composite dynamic load are described. Finally, the impact of composite dynamic load on the DSR is summarized.
Medical and Biomechanical Risk Factors for Incident Bone Stress Injury in Collegiate Runners: Can Plantar Pressure Predict Injury? (2022)

The models collectively suggested that higher plantar pressure may contribute to risk for BSI.
Integrated structure for a resonant micro-gyroscope and accelerometer (2014)

This paper presents the study of the mechanical behavior of a microstructure designed to detect acceleration and angular velocity simultaneously. The new resonant micro-sensor proposed, fabricated by the ThELMA© surface-micromachining technique, bases detection of two components of external acceleration (one in-plane component and one out-of plane component) and two components of angular velocity (roll and yaw) on the variation of frequency of several elements set in resonance. The device, despite its small dimensions, provides a differential decoupled detection of four external quantities thanks to the presence of four slender beams resonating in bending and four torsional resonators.
Crazy Sequential Representations: Base 16 (-FFFFF up to FFFFF) (2019)

Abstract not available.
ChemInform Abstract: Noble Metals in Polyoxometalate Chemistry: Palladium‐Containing Derivatives of the Monolacunary Keggin and Wells—Dawson Tungstophosphates. (2011)

Abstract Compound (III) crystallizes in the monoclinic space group P2 1 /c with Z = 4.
Preparation, Characterization and Visualization of CPP-Mediated MR Contrast Agents (2005)

Recent developments in MR imaging have enabled in vivo imaging at near microscopic resolution. In order to visualize and track cells by MR imaging, it is necessary to tag cells magnetically. Cell-penetrating peptides (CPPs) have been used as an efficient way of internalizing a number of marker proteins into cells. Here we describe the synthesis and testing of a series of bi-labeled (magnetic and fluorescent) Gd(III)-based MR contrast agents conjugated to fluorescent dye and CPPs, including l-Tat49-57, d-Tat57-49, PTD-4 and NLS (Fig. 1). The CPP fragments were synthesized by solid phase with the Fmoc (9-fluorenylmethoxycarbonyl) mediated scheme. FITC (fluorescein isothiocyanate) was coupled to Fmoc-lysine at first. Then FITC-Fmoc-lysine and diethylenetriaminepenta-acetic dianhydride (DTPA dianhydride) were coupled to CPPs, respectively. Finally the conjugates were chelated with Gd3+. The products were purified by reversed-phase HPLC and characterized by ESI-MS. Cellular uptake of these agents were confirmed by fluorescent microscopy and spectroscopy, as well as by T1 and T2 MR analysis of the Gd(III) agents in NIH 3T3 fibroblasts. Further optical and MR evaluation is under progress. The comparison of these different CPP conjugates can provide helpful data for the design of new intracellular MR contrast agents for in vivo tracking of cells.
Determination of insulin in serum by enzyme immunoassay with fluorimetric detection (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDetermination of insulin in serum by enzyme immunoassay with fluorimetric detectionWilliam D. Hinsberg, Kristin H. Milby, and Richard N. ZareCite this: Anal. Chem. 1981, 53, 9, 1509–1512Publication Date (Print):August 1, 1981Publication History Published online1 May 2002Published inissue 1 August 1981https://pubs.acs.org/doi/10.1021/ac00232a048https://doi.org/10.1021/ac00232a048research-articleACS PublicationsRequest reuse permissionsArticle Views183Altmetric-Citations25LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Cylinder Pressure Data from a Heavy Duty Diesel Engine (2015)

Data includes various measurements from a heavy duty diesel engine measured at University of Michigan. Steady state data for the air path variables and crank angle resolved cylinder pressure data are provided. A code in MATLAB is also included as an example for reading and processing the data.
The different domains of the point spread function ‐ Small angle vs large angle (2009)

Abstract Purpose The Point‐Spread‐Function peaks at 107, and drops off to 10‐2 at 90 degrees, a dynamic range of 109. Important changes in the PSF already occur within the first 1 minute of arc, so spatially the dynamic range is 105. Very different experimental techniques are needed to describe the small angle (aberrometry, double pass) and the large angle (straylight) domains. What optical irregularities achieve such PSF? Methods Literature models for the aberration structure of the human eye media, in particular Thibos et al. JOSA A 2002 were used to derive the PSF. The Thibos et al. model was extrapolated to allow inclusion of the high orders of aberration not normally included in aberrometry, but potentially visible with double pass, so as to encompass as much as possible from the PSF. Results The modeled aberration structures of the eye covers the central part of the PSF up till about 0.3 degrees if extreme high orders were included. For angles &gt;1 degree predicted values were far below the actual values of the PSF. On the other hand, double pass techniques grossly overestimate PSF values at angles of 0.3 degree and above. Using an alternative model for the aberration structure of the eye the PSF could be described up till 1 degree. Small particle scattering was essential to predict the PSF for angles above 1 degree. Conclusion In the assessment of disturbances to the optical media two domains must be discriminated: the aberration domain and the small particle domain, with corresponding parts (small angle vs large angle) to the PSF. Straylight originates from irregularities of small characteristic size (10 micrometer and below), as opposed to disturbances to the central part of the psf (&lt;0.3 degrees), originating from large scale irregularities. Commercial interest
Seamless access networks using radio-over-fiber technology for high-speed trains (2016)

This paper reviews seamless networks based on radio-over-fiber (RoF) technology. We focus on train communication networks (TCNs) consisting of optical networks and many radio access units (RAUs). Waveform for radio communications can be effectively delivered to each RAU by RoF. The TCN can be controlled by train location information to reduce overhead due to hand-over in mobile communication systems.
Myeloid-Specific Tristetraprolin Deficiency in Mice Results in Extreme Lipopolysaccharide Sensitivity in an Otherwise Minimal Phenotype (2012)

Tristetraprolin (TTP) is a mRNA-destabilizing protein that binds to AU-rich elements in labile transcripts, such as the mRNA encoding TNF, and promotes their deadenylation and degradation. TTP-deficient (knockout [KO]) mice exhibit an early-onset, severe inflammatory phenotype, with cachexia, erosive arthritis, left-sided cardiac valvulitis, myeloid hyperplasia, and autoimmunity, which can be prevented by injections of anti-TNF Abs, or interbreeding with TNF receptor-deficient mice. To determine whether the excess TNF that causes the TTP KO phenotype is produced by myeloid cells, we performed myeloid-specific disruption of Zfp36, the gene encoding TTP. We documented the lack of TTP expression in LPS-stimulated bone marrow-derived macrophages from the mice, whereas fibroblasts expressed TTP mRNA and protein normally in response to serum. The mice exhibited a minimal phenotype, characterized by slight slowing of weight gain late in the first year of life, compared with the early-onset, severe weight loss and inflammation seen in the TTP KO mice. Instead, the myeloid-specific TTP KO mice were highly and abnormally susceptible to a low-dose LPS challenge, with rapid development of typical endotoxemia signs and extensive organ damage, and elevations of serum TNF levels to 110-fold greater than control. We conclude that myeloid-specific TTP deficiency does not phenocopy complete TTP deficiency in C57BL/6 mice under normal laboratory conditions, implying contributions from other cell types to the complete phenotype. However, myeloid cell TTP plays a critical role in protecting mice against LPS-induced septic shock, primarily through its posttranscriptional regulation of TNF mRNA stability.
Fast and accurate behavioral simulation of fractional-N frequency synthesizers and other PLL/DLL circuits (2002)

Techniques for fast and accurate simulation of fractional-N synthesizers at a detailed behavioral level are presented. The techniques allow a uniform time step to be used for the simulator, and can be applied to a variety of phase locked loop (PLL) and delay locked loop (DLL) circuits beyond fractional-N synthesizers, as well as to a variety of simulation frameworks such as Verilog and Matlab. Simulated results from a custom C++ simulator are shown to compare well to measured results from a prototype fractional-N synthesizer using a /spl Sigma/-/spl Delta/ modulator to dither its divide value.
Astrocytes are GABAergic cells that modulate microglial activity (2010)

GABA is assumed to function in brain only as an inhibitory neurotransmitter. Here we report a much broader CNS role. We show that human astrocytes are GABAergic cells, and that human microglia are GABAceptive cells. We show that in adult human brain tissue, astrocytes immunostain for the GABA synthesizing enzyme GAD 67, the GABA metabolizing enzyme GABA-T and the GABA(A) and GABA(B) receptors. The intensity of staining is comparable or greater to that observed for known inhibitory neurons. We show that cultured human astrocytes strongly express the mRNA and protein for GAD 67, as well as GABA-T, and the GABA(A) and GABA(B) receptors. We further show that cultured human microglia express the mRNA and protein for GABA-T, in addition to the GABA(A) and GABA(B) receptors characterizing them as GABAceptive cells. We demonstrate that GABA suppresses the reactive response of both astrocytes and microglia to the inflammatory stimulants lipopolysaccharide (LPS) and interferon-γ by inhibiting induction of inflammatory pathways mediated by NFκB and P38 MAP kinase. This results in a reduced release of the inflammatory cytokines TNFα and IL-6 and an attenuation of conditioned medium neurotoxicity toward neuroblastoma SH-SY5Y cells. These inhibitory reactions are partially mimicked by the GABA(A) receptor agonist muscimol and the GABA(B) receptor agonist baclofen, indicating that GABA can stimulate both types of receptors in astrocytes as well as microglia. We conclude that the antiinflammatory actions of GABA offer new therapeutic opportunities since agonists should enhance the effectiveness of other antiinflammatory agents that operate through non-GABA pathways.
Tunneled Peritoneal Catheter Placement Under Sonographic and Fluoroscopic Guidance in the Palliative Treatment of Malignant Ascites (2001)

Percutaneous placement of specialized tunneled catheters appears to be a viable and safe technique in patients who have symptomatic ascites that require frequent therapeutic paracentesis for relief of symptoms.