gebhart/patrepeval huggingface dataset:
Transcriptional activation of the translocated c- myc oncogene in Burkitt lymphoma (1983)

We have previously demonstrated that translocations of V(H) genes from chromosome 14 to chromosome 8 and of the c-myc oncogene from chromosome 8 to chromosome 14 occur in Burkitt lymphomas with the t(8;14) chromosome translocation. An association of the c-myc gene with the C(mu) immunoglobulin gene has been observed in some but not all Burkitt lymphomas studied previously. In the present study, we have investigated the organization of the human heavy chain locus and of the c-myc gene in the P3HR-1 Burkitt lymphoma cell line. Becuase mouse/P3HR-1 somatic cell hybrids that retain only the 14q+ chromosome and no other human chromosome contain the human C(mu) and C(gamma) genes but not V(H) genes, we have concluded that the breakpoint on chromosome 14 in P3HR-1 cells is distal to C(mu) and between C(mu) and V(H). Thus, the breakpoint of human chromosome 14 differs in different Burkitt lymphoma cell lines. We also found that the human c-myc oncogene translocated to chromosome 14 in the P3HR-1 cell line is not recombined with the C(mu) gene. The breakpoint on human chromosome 8 may therefore also differ in different Burkitt lymphoma cell lines, because we have observed DNA rearrangement of the c-myc gene with the C(mu) gene in only some of the Burkitt lymphoma cell lines studied elsewhere. Interestingly, high levels of transcripts of the c-myc oncogene were observed in Burkitt lymphomas with translocated c-myc oncogenes both rearranged and unrearranged. Therefore, the translocation of a c-myc oncogene to the heavy chain locus on human chromosome 14 is apparently sufficient for its transcriptional activation and may be an essential step in the pathway leading to neoplasia.
Enhanced Survivability of Translucent Elastic Optical Network Employing Shared Protection with Fallback (2017)

We propose a novel shared-protection scheme for elastic optical networks employing virtualized elastic regenerators that provides almost the same high degree of survivability as dedicated protection even when double-link failures occur while saving backup resources.
Monolithic MOS high voltage integrated circuits (1980)

Two general classes of high voltage MOS IC's may be defined. The first is structured around a conventional NMOS or PMOS technology and adds ion implanted extended drain regions, offset or stacked gates, or lightly doped drain regions to produce high voltage (50-300 volt) output devices. Such devices are generally operated with a grounded source, have an enclosed geometry, are relatively high on-resistance, and have found some application primarily in display driving. A second, more flexible technology is structured around lateral DMOS devices similar to the discrete power FET devices now widely available commercially. This technology generally uses N <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-</sup> epitaxial layers on P <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-</sup> substrates, junction isolation, and is compatible with high voltage PMOS and N channel DMOS devices, and low voltage logic devices. This paper will primarily address the latter class of circuits and will consider practical problems of high voltage device design, power dissipation limitations, parasitic surface and bulk devices, and approaches to level shifting low level logic inputs to high voltages.
Toxicity Assays in Nanodrops Combining Bioassay and Morphometric Endpoints (2007)

Using pre/sub lethal cell stress instead of cell mortality, we highlighted the high significance and the superior sensitivity of both stress promoter activation reporting and cell morphology parameters in measuring the cell response to a toxicant. These results demonstrate the first generation of high-throughput and high-content assays, capable of assessing chemical hazards in vitro within the REACH policy framework.
Advances in lanthanide‐based luminescent peptide probes for monitoring the activity of kinase and phosphatase (2013)

Abstract Signaling pathways based on protein phosphorylation and dephosphorylation play critical roles in the orchestration of complex biochemical events and form the core of most signaling pathways in cells (i.e. cell cycle regulation, cell motility, apoptosis, etc.). The understanding of these complex signaling networks is based largely on the biochemical study of their components, i.e. kinases and phosphatases. The development of luminescent sensors for monitoring kinase and phosphatase activity is therefore an active field of research. Examples in the literature usually rely on the modulation of the fluorescence emission of organic fluorophores. However, given the exceptional photophysical properties of lanthanide ions, there is an increased interest in their application as emissive species for monitoring kinase and phosphatase activity. This review summarizes the advances in the development of lanthanide‐based luminescent peptide sensors as tools for the study of kinases and phosphatases and provides a critical description of current examples and synthetic approaches to understand these lanthanide‐based luminescent peptide sensors.
Vacancy Modulating Co3Sn2S2 Topological Semimetal for Aqueous Zinc‐Ion Batteries (2021)

Weyl semimetals (WSMs) with high electrical conductivity and suitable carrier density near the Fermi level are enticing candidates for aqueous Zn-ion batteries (AZIBs), meriting from topological surface states (TSSs). We propose a WSM Co<sub>3</sub> Sn<sub>2</sub> S<sub>2</sub> cathode for AZIBs showing a discharge plateau around 1.5 V. By introducing Sn vacancies, extra redox peaks from the Sn<sup>4+</sup> /Sn<sup>2+</sup> transition appear, which leads to more Zn<sup>2+</sup> transfer channels and active sites promoting charge-storage kinetics and Zn<sup>2+</sup> storage capability. Co<sub>3</sub> Sn<sub>1.8</sub> S<sub>2</sub> achieves a specific energy of 305 Wh kg<sup>-1</sup> (0.2 Ag<sup>-1</sup> ) and a specific power of 4900 Wkg<sup>-1</sup> (5 Ag<sup>-1</sup> ). Co<sub>3</sub> Sn<sub>1.8</sub> S<sub>2</sub> and Zn<sub>x</sub> Co<sub>3</sub> Sn<sub>1.8</sub> S<sub>2</sub> benefit from better conductivity at lower temperatures; the quasi-solid Co<sub>3</sub> Sn<sub>1.8</sub> S<sub>2</sub> //Zn battery delivers 126 mAh g<sup>-1</sup> (0.6 Ag<sup>-1</sup> ) at -30 °C and a cycling stability over 3000 cycles (2 Ag<sup>-1</sup> ) with 85 % capacity retention at -10 °C.
ChemInform Abstract: SOLVOLYTIC REARRANGEMENT OF 1,2,3‐TRI‐TERT.‐BUTYL‐3‐DICHLOROMETHYLCYCLOPROPENE AND CIS‐3,4‐DICHLORO‐1,2,3‐TRI‐TERT.‐BUTYLCYCLOBUTENE (1973)

Abstract Das Cyclopropenylfluoroborat (I) reagiert mit Dichlormethyllithium zum Cyclopropen (II), das sich bei 150°C zum Cyclobuten (III) isomerisiert.
Distinguishing the efficiency requirements in motoring and generating operations of PM machines (2016)

The influences of stator winding resistance and dc bus voltage variations on the efficiency maps of permanent magnet machines for motoring and generating operations are discussed. A large-scale design optimization algorithm is presented in which the efficiency requirements are independently treated for motoring and generating operations over the entire torque-speed plane. The separate treatment of efficiency is especially important in machines with large per-unit stator winding resistances, or in machine-drive systems with unregulated dc bus voltage, as is the case for low-voltage hybrid vehicle systems.
The arterial complications of polycythaemia vera (1973)

Abstract Of 200 patients referred for management of polycythaemia vera, 98 (49 per cent) had presented with vascular complications and 68 (34 per cent) of these involved arteries. Transient but recurrent cerebrovascular accidents and digital artery occlusions were the typical arterial complications. If polycythaemia was not promptly treated there was a risk of perimanent hemiplegia, monocular blindness, and severe ischaemia of the leg. Embolism may have accounted for some of the arterial complications. A possible source for emboli—a proximal mural thrombus—was demonstrated in all 5 patients who were examined with femoral arteriography and in 2 patients who underwent surgery. In selected cases thrombectomy may be indicated to prevent further embolism.
Limiting Cardiac Ischemic Injury by Pharmacological Augmentation of Macrophage Migration Inhibitory Factor–AMP-Activated Protein Kinase Signal Transduction (2013)

These data support the pharmacological utility of small-molecule MIF agonists in enhancing AMPK activation and reducing cardiac ischemic injury.
Microsphere Composites of Nano-Al and Nanothermite: An Approach to Better Utilization of Nanomaterials (2014)

Abstract : Nanometallic fuels suffer from processing challenges that have significantly retarded their utility, primarily because their very high surface area/small particle size increases the viscosity of the polymer binder and oxidizer mix, such that high mass fractions of fuel cannot be formulated. The other concern is that they lose surface area rapidly, on a time-scale commensurate with combustion. We employ electrospray as a means to create a gel within a droplet by evaporation induced rapid aggregation of aluminum and oxidizer nanoparticles containing a small mass fraction of an energetic binder. We find that the average size of the microparticles can be systematically changed from 2 microns to 16 microns. The combustion behavior is found to be very different from either nanoaluminum or micron aluminum and their corresponding thermite mixtures. The material which is super-micron sized has surface area consistent with the nanoparticle comprising it and the binder serves to act as a gas generator to minimize sintering.
ChemInform Abstract: New General Processes of Homolytic Alkylation of Heteroaromatic Bases by t‐BuOOH or (t‐BuO)2 and Alkyl Iodides. (1990)

Abstract Selective homolytic alkylation is observed in the presence of methyl radicals generated from Me 3 C‐OOH and (Me 3 C‐O)2.
Integrating assist feature print fixing with OPC (2009)

A challenge in model-based assist feature placement is to find optimal placements while satisfying mask rules and preventing AF printing. There are numerous strategies for achieving this ranging from fully rule-based methods to pixel-based inversion. Our proposed solution is to identify the optimal locations of assist features using modeling information based strictly on optics and resist stack optical characteristics. Once these positions have been found, preliminary AFs can be placed. At this point suggested sizes and shapes can be identified, although these can later be modified. In a later step, MRC cleanup, printability fixing, and main-pattern OPC can be performed simultaneously. This has the advantage of allowing the use of the full process model to predict the location of OPC edges accurately, and use calibrated or 3d mask models to determine assist feature printing behavior. This correction is done while maintaining MRC constraints. In this flow, an AF placement field, generated from the pre-OPC target patterns, can be used to provide accurate guidance on how to move assist features to get the most benefit while keeping other constraints in mind. Using this method, a range of printability fixing strategies, guided by placement benefits, is available. We present data showing that the benefit of AF placements can be determined from optical parameters, on target (non-OPC) data, and that this method leads to beneficial yet compliant masks.
Bioluminescence imaging of alpha-synuclein oligomerization in mouse brain with split firefly luciferase reporters (2013)

status: Published
In Vivo Deactivation of Proinsulin Action on Glucose Disposal and Hepatic Glucose Production in Normal Man (1986)

We have studied the deactivation of the in vivo actions of insulin and biosynthetic human proinsulin (recombinant DNA) to stimulate the glucose disposal rate (GDR) and to inhibit hepatic glucose output (HGO) in man. Twelve healthy, lean, young subjects were studied using a modification of the euglycemic glucose clamp technique. Subjects received 4-h infusions on separate occasions of insulin (15 mU/m2/min equivalent to 0.54 microgram/m2/min) or proinsulin (2.75 micrograms/m2/min), achieving steady-state serum levels of 32 +/- 3 microU/ml (equivalent to 0.23 +/- 0.02 pmol/ml) and 3.7 +/- 0.2 pmol/ml, respectively. Suppression of HGO was similar (83-84%) with proinsulin and insulin, but stimulation of GDR above basal was greater with insulin (3.41 +/- 0.43 versus 1.98 +/- 0.28 mg/kg/min, P less than 0.001). Following cessation of the hormone infusions, serum proinsulin concentration fell in a biphasic fashion with half-times of 25 and 146 min for the two phases. Serum half-disappearance time for insulin was 5 min. Deactivation of the hormone's effects to stimulate GDR was 50% complete by 35 min after insulin and 71 min after proinsulin. In contrast, 50% of the recovery times for the effect on suppression of HGO were 55 min after insulin and 188 min after proinsulin. Serum glucagon levels did not differ significantly after the insulin and proinsulin infusions. In summary: (1) Deactivation of proinsulin and insulin's effects to suppress HGO proceeds more slowly than deactivation of their effects to stimulate GDR; and (2) There is a markedly prolonged and disproportionately delayed deactivation of proinsulin's effects on suppression of HGO. This later finding may prove of therapeutic value in the treatment of diabetes mellitus.
Quantitative PCR-Based Measurement of Nuclear and Mitochondrial DNA Damage and Repair in Mammalian Cells (2014)

In this chapter, we describe a gene-specific quantitative PCR (QPCR)-based assay for the measurement of DNA damage, using amplification of long DNA targets. This assay has been used extensively to measure the integrity of both nuclear and mitochondrial genomes exposed to different genotoxins and has proven to be particularly valuable in identifying reactive oxygen species-mediated mitochondrial DNA damage. QPCR can be used to quantify both the formation of DNA damage as well as the kinetics of damage removal. One of the main strengths of the assay is that it permits monitoring the integrity of mtDNA directly from total cellular DNA without the need for isolating mitochondria or a separate step of mitochondrial DNA purification. Here we discuss advantages and limitations of using QPCR to assay DNA damage in mammalian cells. In addition, we give a detailed protocol of the QPCR assay that helps facilitate its successful deployment in any molecular biology laboratory.
Neural Network Approaches for Soft Biological Tissue and Organ Simulations (2022)

Given the functional complexities of soft tissues and organs, it is clear that computational simulations are critical in their understanding and for the rational basis for the development of therapies and replacements. A key aspect of such simulations is accounting for their complex, nonlinear, anisotropic mechanical behaviors. While soft tissue material models have developed to the point of high fidelity, in-silico implementation is typically done using the finite element (FE) method, which remains impractically slow for translational clinical time frames. As a potential path toward addressing the development of high fidelity simulations capable of performing in clinically relevant time frames, we review the use of neural networks (NN) for soft tissue and organ simulation using two approaches. In the first approach, we show how a NN can learn the responses for a detailed meso-structural soft tissue material model. The NN material model not only reproduced the full anisotropic mechanical responses but also demonstrated a considerable efficiency improvement, as it was trained over a range of realizable fibrous structures. In the second approach, we go a step further with the use of a physics-based surrogate model to directly learn the displacement field solution without the need for raw training data or FE simulation datasets. In this approach we utilize a finite element mesh to define the domain and perform the necessary integrations, but not the finite element method (FEM) itself. We demonstrate with this approach, termed neural network finite element (NNFE), results in a trained NNFE model with excellent agreement with the corresponding "ground truth" FE solutions over the entire physiological deformation range on a cuboidal myocardium specimen. More importantly, the NNFE approach provided a significantly decreased computational time for a range of finite element mesh sizes. Specifically, as the FE mesh size increased from 2744 to 175,615 elements, the NNFE computational time increased from 0.1108 s to 0.1393 s, while the "ground truth" FE model increased from 4.541 s to 719.9 s, with the same effective accuracy. These results suggest that NNFE run times are significantly reduced compared with the traditional large-deformation-based finite element solution methods. We then show how a nonuniform rational B-splines (NURBS)-based approach can be directly integrated into the NNFE approach as a means to handle real organ geometries. While these and related approaches are in their early stages, they offer a method to perform complex organ-level simulations in clinically relevant time frames without compromising accuracy.
Enhanced LMS based Feedback Loop Interference Cancellation in DVB-H Systems with Gap Fillers (2007)

In this paper, we present an enhanced least mean square (LMS) based feedback loop interference canceller algorithm intended to be used in digital terrestrial broadcasting single frequency network (SFN) via single on-channel repeater system (OCR). The feedback loop interference is caused by the coupling that could exist between transmitting and receiving antennas. The performance of the method is evaluated in BER sense assuming a Rayleigh channel between the main transmitter and the repeater with multi-tap feedback loop having either Ricean or Rayleigh profile.
NT-07 * PHASE 1-2 DOSE-ESCALATION STUDY OF VB-111, AN ANTI-ANGIOGENIC GENE THERAPY, AS MONOTHERAPY AND IN COMBINATION WITH BEVACIZUMAB, IN PATIENTS WITH RECURRENT GLIOBLASTOMA (2014)

BACKGROUND: VB-111 is an anti-angiogenic agent consisting of a non-replicating adenovirus vector (Ad-5) with a modified murine pre-proendothelin promoter leading to apoptosis of tumor vasculature by expressing a fas-chimera transgene in angiogenic endothelial cells. Safety and efficacy of VB-111 alone and in combination with bevacizumab (BEV) were evaluated for patients with recurrent Glioblastoma (rGBM) in this phase 1-2 dose-escalation study. METHODS: VB-111 was administered as a single intravenous infusion at escalating doses from 1x1012 to 1x1013 viral particles (VPs), followed by repeat doses of 3x1012 or 1x1013 every 2 months. The protocol was amended to add-on BEV 10mg/Kg every 2 weeks upon further progression. Assessments included safety, pharmacokinetics, tumor response (RANO criteria) and overall survival (OS). RESULTS: Forty-six patients at 4 recruiting medical centers in the US and Israel received up to 13 repeat doses of VB-111. Of these 30 received the high dose (1x1013). There were 22 related adverse events, 19 CTCAE grade 1-2. The median OS was 360 [range: 70-574] and 266 days [range: 28-664] for patients receiving at least one high dose vs. subjects who received lower doses, respectively (p NS). Progression free survival was 63 vs. 55 days for patients who received high vs. lower doses, respectively (p = 0.01). Median follow-up was 232 days. Six patients had a partial response and/or prolonged disease stability (≥180 days). Tumor growth rates showed a statistically significant dose response. Eleven patients received combination therapy of VB-111 with BEV after progression on VB-111 alone. Median time to second progression was 93 days. VB-111 was safe and well tolerated both as monotherapy and combined therapy. CONCLUSIONS: VB-111 was safe and well tolerated as monotherapy and in combination with BEV in patients with recurrent glioblastoma. Encouraging tumor growth attenuation and responses were seen. Overall survival was about 3 months longer than historical rGBM data.
Increased Cholecystectomy Rate After Introduction of Laparoscopic Cholecystectomy-Reply (1994)

<h3>In Reply.</h3> —We appreciate the comments of Foster et al and definitely agree with them that one should be careful not to generalize results when using a "relatively small convenience sample"; however, we do not think that a private practice— based HMO having 500 000 enrollees is a small sample. The national estimated rates reported in the 1991 NHDS were based on a medical record survey sample.<sup>1</sup>In our study, we used a complete sample of US Healthcare's HMO enrollees in southeastern Pennsylvania. Although it is possible that the increase in the cholecystectomy rate might have been an isolated phenomenon related only to the population that we studied—as suggested by Foster et al—there is much evidence to suggest otherwise. Orlando et al<sup>2</sup>documented a 29% increase in cholecystectomies in the state of Connecticut after the introduction of the laparoscopic approach. Drs Klar and Kongstvedt report a 52.4% increase
Metabolite profiling of Calvin cycle intermediates by HPLC‐MS using mixed‐mode stationary phases (2008)

A sensitive and robust mixed-mode high performance liquid chromatography-tandem mass spectrometry method was developed for the qualitative and quantitative determination of sugar phosphates, which are notoriously difficult to separate using reversed-phase materials. Sugar phosphates were separated on a Primesep SB column by gradient elution using aqueous ammonium formate and acetonitrile as mobile phases. Target analytes were identified by their precursor/product ions and retention times. Quantitative analysis was performed in negative ionization/multiple reaction monitoring mode with five different time segments. The method was validated by spiking authentic sugar phosphate standards into complex plant tissue extracts. Standard curves of neat authentic standards and spiked extracts were generated for concentrations in the low picomole to nanomole range, with correlation coefficients of R(2) > 0.991, and the degree of ion suppression in the presence of a plant matrix was calculated for each analyte. Analyte recoveries, which were determined by including known quantities of authentic standards in the sugar phosphate extraction protocol, ranged from 40.0% to 57.4%. The analytical reproducibility was assessed by determining the coefficient of variance based on repeated extractions/measurements (<20%). The utility of our method is demonstrated with two types of applications: profiling of Calvin cycle intermediates in (i) dark-adapted and light-treated tobacco leaves, and in (ii) antisense plants expressing reduced levels of the Calvin cycle enzymes glyceraldehyde-3-phosphate dehydrogenase or ribulose-1,5-bisphosphate carboxylase/oxygenase (comparison with wild-type controls). The broader applicability of our method is illustrated by profiling sugar phosphates extracted from the leaves of five taxonomically diverse plants.