gebhart/patrepeval huggingface dataset:
Subsea Compression – Project Overview (2016)

2015 represents an important milestone for Statoil as both the Gullfaks and Åsgard subsea compression projects achieved production start-up. The Gullfaks subsea wet gas compression system is projected to increase recovery from the Gullfaks South Brent reservoir by 22 million barrels of oil equivalent. The solution involves two 5 megawatt wet gas compressors that handle a production rate of 10 million standard cubic metres of gas per day. The compressor system is connected to existing subsea templates and piping 15 kilometres from Gullfaks C. The Åsgard subsea compression system is projected to provide 306 million barrels of oil equivalent in increased recovery from the Mikkel and Midgard fields. The solution involves two 11.5 megawatt centrifugal compressors that handle a production rate of 21 million standard cubic metres of gas per day. The compressor system is connected to existing subsea templates and piping 40 kilometres from Åsgard B. The difference in scale and boosting requirements helps to explain why two quite different technical solutions have been selected. This presentation will provide an overview of the two projects including: Why subsea compression The business cases and technology selection The technology assessment and qualification. The execution program for the projects including engineering, procurement, construction, testing and installation. Commissioning and start up Future subsea compression opportunities
Recent Progress in Graphene-Related Nanotechnologies (2009)

Currently there is great interest in graphene-based devices and applications. The main advantages of graphene include excellent conductive and mechanical properties. The applications of graphene cover a wide range of possibilities, from next-generation transistors to light-weight, high-strength composite materials. However, commercial use of graphene will depend on the development of an industrially-viable method of fabricating and handling graphene. The recent advances in manipulating graphene and patents will be reviewed with a focus on the progress of graphene nanoparticle synthesis and applications.
Abstract B139: Toxicity testing of EGFRvIII CAR-based immunotherapy of glioblastoma: From bench to bedside (2016)

Abstract The purpose of this study was to generate a panel of donor-derived primary cells, expand them ex-vivo in to sufficient numbers to utilize as targets for evaluating potential normal tissue toxicity of epidermal growth factor receptor mutation variant three (EGFRvIII)-specific chimeric antigen receptors (CAR) prior to use in clinical trials for patients with glioblastoma (GBM). Nine cell types were obtained, including different epithelial, endothelial, bone, smooth muscle, cardiac, neural, hematopoetic, stem cells, and keratinocytes. Cells were expanded with individual specialized media and protocols for between 8-12 passages. After expansion, primary cell identity was confirmed by morphology, ICC and IHC for characteristic markers. Levels of EGFR and EGFRvIII in each cell type were determined by qRT-PCR. To evaluate potential normal-cell toxicity, CAR T cells were co-cultured with each type of primary cell and function was evaluated in two ways: i) T cell activation was measured by staining and flow cytometry of CD3+ T cells stained intracellularly for CD107a, or GzmB, TNFalpha, IFNgamma, IL-2 cytokines. ii) Target cell lysis was evaluated by labeling primary cells with 51Cr prior to 4 hour co-culture with increasing numbers of CAR T cells, and measuring chromium-release. None of the primary cells showed expression of EGFRvIII, although several, in particular keratinocytes and renal epithelial, had high levels of EGFR. In functional assays, while the EGFR CAR T cells recognized and lysed EGFR expressing cell types, EGFRvIII CARs showed T cell activation and target lysis only of EGFRvIII expressing tumors. EGFRvIII CAR 2173 was selected for use in clinical trials at UPENN and UCSD, treating patients with GBM. To date, 6 patients have been infused with 2173 EGFRvIII CAR T cells, with no observed toxicity. All patients had detectable expansion of CAR T cells in vivo in blood, and one patient with subsequent tumor resection had detectable intra-tumoral CAR T cells. These CARs appear to be safe, persist in vivo and traffic into GBM tumor. An update on the clinical trial will be presented at the conference. Note:This abstract was not presented at the conference. Citation Format: Alexandria P. Cogdill, Alina Boesteanu, Chong Xu, Kathleen Haines, John Scholler, Joseph Fraietta, Yangbing Zhao, Xiaojun Liu, Jennifer Morrissette, Bruce Levine, Simon Lacey, Andreas Loew, Reshma Singh, Jennifer Brogdon, Donald M. O'Rourke, Marcela V. Maus, Carl H. June, Laura A. Johnson. Toxicity testing of EGFRvIII CAR-based immunotherapy of glioblastoma: From bench to bedside. [abstract]. In: Proceedings of the CRI-CIMT-EATI-AACR Inaugural International Cancer Immunotherapy Conference: Translating Science into Survival; September 16-19, 2015; New York, NY. Philadelphia (PA): AACR; Cancer Immunol Res 2016;4(1 Suppl):Abstract nr B139.
Self-assembled carbon nanotubes in a microtrap for chemical sensing and chromatography (2005)

Self-assembled carbon nanotubes in a microtrap for chemical sensing and chromatography / httP://isiknowledge.com
6. Abkürzungsverzeichnis (2019)

Mit Musik gemeinsam gegen das Trauma: Rhythmisches Musizieren in der Gruppe zielt als nonverbale traumapädagogische Intervention auf die Förderung und Steigerung der (Selbst-)Heilungsressourcen gewalterfahrener und traumatisierter Jugendlicher in der stationären Kinder- und Jugendhilfe.
AI-Augmented Multi Function Radar Engineering with Digital Twin: Towards\n Proactivity (2020)

Thales new generation digital multi-missions radars, fully-digital and\nsoftware-defined, like the Sea Fire and Ground Fire radars, benefit from a\nconsiderable increase of accessible degrees of freedoms to optimally design\ntheir operational modes. To effectively leverage these design choices and turn\nthem into operational capabilities, it is necessary to develop new engineering\ntools, using artificial intelligence. Innovative optimization algorithms in the\ndiscrete and continuous domains, coupled with a radar Digital Twins, allowed\nconstruction of a generic tool for "search" mode design (beam synthesis,\nwaveform and volume grid) compliant with the available radar time budget. The\nhigh computation speeds of these algorithms suggest tool application in a\n"Proactive Radar" configuration, which would dynamically propose to the\noperator, operational modes better adapted to environment, threats and the\nequipment failure conditions.\n
Electrical and physical characterization of remote plasma oxidized HfO/sub 2/ gate dielectrics (2006)

Bi-layer gate stacks consisting of a HfO/sub 2/ and an interfacial layer are fabricated by remote plasma oxidation (RPO) of Hf metal deposited on an Si substrate. Hf metal is fully oxidized by the RPO even at a temperature as low as 400/spl deg/C due to radical oxygens, leading to an improvement in the quality of HfO/sub 2/ with less impact to the interfacial layer growth. An insufficient oxidation leads to a deterioration of mobility with increasing interface traps and positive bias temperature instability, which is likely caused by the oxygen vacancies acting as traps induced by the remaining Hf metal. The SiO/sub 2/-like interface improves the mobility with reduced interface states. Full oxidation and the controlled SiO/sub 2/-like interface demonstrate RPO as a promising way for gate-stack optimization.
Apoptotic Signaling in Male Germ Cells (2011)

Programmed germ cell death (apoptosis) is conspicuous during normal spermatogenesis and serves as a quality control system for the production of normal sperm. Deregulation of germ cell death is associated with defective spermatogenesis and impaired fertility. Mitochondria-dependent intrinsic pathway signaling constitutes a critical component of apoptotic signaling in male germ cells across species. However, the regulation of germ cell apoptosis may vary depending on the nature of the apoptotic stimulus and can be triggered by more than one pathway. Activation of p38 mitogen-activated protein kinase (MAPK) and induction of inducible nitric oxide synthase are critical for activation of the intrinsic pathway signaling in male germ cells. In addition, there is increasing evidence that caspase-2 is an upstream activator of the p38 MAPK and nitric oxide–mediated intrinsic pathway signaling. This chapter focuses on the recent progress in our understanding of the regulation of germ cell apoptosis in the testis.
Fourier modeling of porcine heartbeat and respiration in vivo for synchronization of HeartLander robot locomotion (2011)

HeartLander is a small mobile robot which adheres to and navigates over the surface of the heart to provide therapies in a minimally invasive manner. HeartLander's ability to efficiently operate in this dynamic environment is greatly affected by physiological motion, namely the cardiac and respiration cycles. Synchronization of robot motion with minimal intrapericardial pressure results in safer and more efficient travel. The work presented models the physiological components of motion using Fourier series and estimates their parameters using an Extended Kalman Filter. Using the Fourier series parameters, estimates of physiological phase values are calculated to be used for step synchronization. The proposed methods are demonstrated on data from a HeartLander animal study for four locations on the heart. Mean respiration phase estimates are shown to be within 5% of the true respiration phases, while mean cardiac phase estimates are shown to have a minimum error of 11%.
Geometrical and Process Effects on MEMS Dimensional Loss: a Frequency Based Characterization (2019)

In this paper we describe a methodology to fully characterize the Deep Reactive Ion Etching (DRIE) of Micro-Electro Mechanical (MEM) structures. One of the key aspects of this manufacturing step is the dimensional loss (CDloss) with respect to nominal geometry. For the sake of simplicity, this parameter is commonly considered uniform in the mechanical element design, leading to a series of approximations. In order to better fashion the local response of the etching process on the mechanical elements, a test pattern of resonating structures was implemented. An in plane resonator using folded springs arranged in a “Tang-like” configuration, shielded with different sets of dummy elements was designed. With an extensive experimental campaign, it has been possible to demonstrate a strong correlation between the first natural frequency of the resonator (i.e. CDloss) with the characteristic dimensions of the shielding elements. Moreover, the effect of different etching equipment was analyzed, showing a significant impact and highlighting the need of different mask design customization for each process tool.
Hybrid photo‐receiver based on SiGe heterojunction photo‐transistor for low‐cost 60 GHz intermediate‐frequency radio‐over‐fibre applications (2015)

The first results of an integrated hybrid photo‐receiver based on a 850 nm two‐terminal (2T) silicon germanium (SiGe) heterojunction bipolar photo‐transistor (HPT) for low‐cost radio‐over‐fibre (RoF) applications is presented. A hybrid module was realised with two cascaded low‐noise amplifiers of 20 dB total gain and an SiGe 2T‐HPT exhibiting a −15 dB opto‐microwave gain at 5.15 GHz with a 1.6 GHz bandwidth. A 16% degradation of the error vector magnitude compared with back‐to‐back measurement for the transmission of a 2 GHz OFDM signal at 3 Gbit/s was measured based on the 60 GHz IEEE 802.15.3c standard transposed at the intermediate frequency (IF) of 5 GHz, according to an IF‐RoF distribution mode.
<title>Ultrahigh-capacity multidata layer ROM</title> (1996)

We have developed an ultra-high-capacity double-sided multi- data-layer ROM that includes additional UV-cured-resin space-layers on two polycarbonate (PC) substrates. This ROM is made using the photo-polymerization (2P) method and has a structure that bonds the two substrates together. In a double-sided dual-data-layer ROM, using the smallest mark size of 0.44 micrometers with a 0.74-micrometer track-pitch for each layer provides a total data capacity of 17 GB using the 8-16 modulation method. Each side of the double-sided dual-sided dual-data-layer ROM consists of a Si-rich silicon nitride semi-reflective layer (layer 0 or 2) on a 570- micrometers-thick PC substrate and an Al reflective layer (layer 1 or 3) on an additional 50-micrometer-thick space layer. We achieved a base jitter of less than 8 percent and a radial tilt margin of 0.7 degree for all four data layers. We also examined the double-sided multi-data-layer ROM disc structure, and demonstrated the feasibility of the double- sided tri-data-layer ROM that includes a total of six layers and has an ultra-high capacity of 25.5 GB.
Effect of latex concentration on epoxidation of natural rubber (NR) latex (1992)

Abstract Effect of latex concentration on the extent of epoxidation and physical properties of epoxidized natural rubber (ENR) was evaluated as a function of latex concentration in the range of 20–60% by weight. The epoxidation rate increased with increasing latex concentration. Physical properties and sequence distribution of an epoxy group of ENR with 30 mol % epoxy content was invariant with the latex concentration employed for epoxidation.
Distinct and opposite effects of leukemogenic Idh and Tet2 mutations in hematopoietic stem and progenitor cells (2023)

Mutations in <i>IDH1,</i> <i>IDH2</i>, and <i>TET2</i> are recurrently observed in myeloid neoplasms. <i>IDH1</i> and <i>IDH2</i> encode isocitrate dehydrogenase isoforms, which normally catalyze the conversion of isocitrate to α-ketoglutarate (α-KG). Oncogenic <i>IDH1/2</i> mutations confer neomorphic activity, leading to the production of D-2-hydroxyglutarate (D-2-HG), a potent inhibitor of α-KG-dependent enzymes which include the TET methylcytosine dioxygenases. Given their mutual exclusivity in myeloid neoplasms, <i>IDH1</i>, <i>IDH2</i>, and <i>TET2</i> mutations may converge on a common oncogenic mechanism. Contrary to this expectation, we observed that they have distinct, and even opposite, effects on hematopoietic stem and progenitor cells in genetically engineered mice. Epigenetic and single-cell transcriptomic analyses revealed that <i>Idh2<sup>R172K</sup></i> and <i>Tet2</i> loss-of-function have divergent consequences on the expression and activity of key hematopoietic and leukemogenic regulators. Notably, chromatin accessibility and transcriptional deregulation in <i>Idh2<sup>R172K</sup></i> cells were partially disconnected from DNA methylation alterations. These results highlight unanticipated divergent effects of <i>IDH1/2</i> and <i>TET2</i> mutations, providing support for the optimization of genotype-specific therapies.
Next-generation sequencing reveals the biological significance of the N[superscript 2],3-ethenoguanine lesion in vivo (2015)

Etheno DNA adducts are a prevalent type of DNA damage caused by vinyl chloride (VC) exposure and oxidative stress. Etheno adducts are mutagenic and may contribute to the initiation of several pathologies; thus, elucidating the pathways by which they induce cellular transformation is critical. Although N[superscript 2],3-ethenoguanine (N[superscript 2],3-εG) is the most abundant etheno adduct, its biological consequences have not been well characterized in cells due to its labile glycosidic bond. Here, a stabilized 2′-fluoro-2′-deoxyribose analog of N[superscript 2],3-εG was used to quantify directly its genotoxicity and mutagenicity. A multiplex method involving next-generation sequencing enabled a large-scale in vivo analysis, in which both N[superscript 2],3-εG and its isomer 1,N[superscript 2]-ethenoguanine (1,N[superscript 2]-εG) were evaluated in various repair and replication backgrounds. We found that N[superscript 2],3-εG potently induces G to A transitions, the same mutation previously observed in VC-associated tumors. By contrast, 1,N[superscript 2]-εG induces various substitutions and frameshifts. We also found that N[superscript 2],3-εG is the only etheno lesion that cannot be repaired by AlkB, which partially explains its persistence. Both εG lesions are strong replication blocks and DinB, a translesion polymerase, facilitates the mutagenic bypass of both lesions. Collectively, our results indicate that N[superscript 2],3-εG is a biologically important lesion and may have a functional role in VC-induced or inflammation-driven carcinogenesis.
Interferon autoantibodies associated with AIRE deficiency decrease the expression of IFN-stimulated genes (2008)

Neutralizing autoantibodies to type I, but not type II, interferons (IFNs) are found at high titers in almost every patient with autoimmune polyendocrinopathy candidiasis ectodermal dystrophy (APECED), a disease caused by AIRE gene mutations that lead to defects in thymic T-cell selection. Combining genome-wide expression array with real time RT-PCR assays, we here demonstrate that antibodies against IFN-alpha cause highly significant down-regulation of interferon-stimulated gene expression in cells from APECED patients' blood by blocking their highly dilute endogenous IFNs. This down-regulation was lost progressively as these APECED cells matured in cultures without neutralizing autoantibodies. Most interestingly, a rare APECED patient with autoantibodies to IFN-omega but not IFN-alpha showed a marked increase in expression of the same interferon-stimulated genes. We also report unexpected increases in serum CXCL10 levels in APECED. Our results argue that the breakdown of tolerance to IFNs in AIRE deficiency is associated with impaired responses to them in thymus, and highlight APECED as another autoimmune disease with associated dysregulation of IFN activity.
Coordination of cohabiting phage elements supports bacteria–phage cooperation (2019)

Bacterial pathogens often carry multiple prophages and other phage-derived elements within their genome, some of which can produce viral particles in response to stress. Listeria monocytogenes 10403S harbors two phage elements in its chromosome, both of which can trigger bacterial lysis under stress: an active prophage (ϕ10403S) that promotes the virulence of its host and can produce infective virions, and a locus encoding phage tail-like bacteriocins. Here, we show that the two phage elements are co-regulated, with the bacteriocin locus controlling the induction of the prophage and thus its activity as a virulence-associated molecular switch. More specifically, a metalloprotease encoded in the bacteriocin locus is upregulated in response to stress and acts as an anti-repressor for CI-like repressors encoded in each phage element. Our results provide molecular insight into the phenomenon of polylysogeny and its intricate adaptation to complex environments.
T cell receptor repertoire in B cell lymphoproliferative lesions in primary Sjögren's syndrome. (1999)

Our observations in SS related lymphoproliferative lesions largely reflect previous evidence in fully benign lesions. The pathogenetic events involved in autoimmune benign lesions in SS may then persist and play a role in SS related lymphoproliferative disorders. The link between the observed TCR-Vbeta repertoire and specific local triggering (auto)antigens remains to be elucidated.
Latent topic driving model for movie affective scene classification (2009)

This paper proposes a latent topic driving model (LTDM) as a novel approach to movie affective scene classification. LTDM is a discriminative model of emotions driven by movie affective contents. Unlike existing methods, our approach is based on movie topic extraction via the latent Dirichlet allocation (LDA) and emotion dynamics modeling with reference to Plutchik's emotion theory. The classification procedure starts by segmenting movie scenes into movie shots, each of which is represented by a histogram of quantized affect-related audio-visual features. LDA is applied to detect topics of each movie shot. Emotions for the current movie shot are estimated based on both the topics of the shot and emotion transition weights determined by Plutchik's emotion theory. We conduct experiments using 206 movie scenes extracted from 24 movie titles (total 6 hours 20 min. 12 sec.) and the labels of eight emotion categories given by 16 subjects are collected. The results show that LTDM outperforms conventional modeling approaches in terms of the subject agreement rate.
Systemic post-translational control of bacterial metabolism regulates adaptation in dynamic environments (2017)

Abstract Across all domains of life, elaborate control mechanisms regulate proteins, pathways, and cell phenotypes as organisms adapt to ever-changing environments. Post-translational modifications (PTMs) allow cells to rapidly and reversibly regulate molecular pathways, but it remains unclear how individual PTMs regulate fitness. Here, we studied &gt;130 PTM sites in Escherichia coli to unravel how PTMs regulate cell metabolism and fitness in response to environmental changes, such as the glucose-acetate diauxie. Using a new metabolic modeling approach, we found a significant fraction of post-translationally modified enzymes are predicted to control shifts in pathway usage following evolutionarily-important environmental changes. Genetic screens using Multiplex Automated Genome Engineering confirmed that these PTMs impact cellular fitness, especially under dynamically changing environments. Finally, mechanisms of how individual PTMs impact protein function were detailed using molecular dynamics simulations and enzyme assays for enolase, transaldolase, and serine hydroxymethyltransferase. Thus, by integrating whole-cell data and pathway modeling with detailed biochemical analysis, we unraveled how individual PTMs regulate enzymes, pathways, and phenotypes to adapt to sudden environmental changes.
Hybrid polyketide synthases (2023)

The present invention provides for a polyketide synthase (PKS) capable of synthesizing an even-chain or odd-chain diacid or lactam or diamine. The present invention also provides for a host cell comprising the PKS and when cultured produces the even-chain diacid, odd-chain diacid, or KAPA. The present invention also provides for a host cell comprising the PKS capable of synthesizing a pimelic acid or KAPA, and when cultured produces biotin.
Stretchable Organic Thin-Film Transistors Fabricated on Elastomer Substrates Using Polyimide Stiff-Island Structures (2014)

We demonstrate stretchable organic thin-film transistors (OTFTs) on a polyimide stiff-island/elastomer substrate. All processes are performed entirely at elastomer-compatible temperatures. We confirm the basic properties of the poly(vinylidene fluoride-trifluoroethylene) [P(VDF-TrFE)] with a poly(methyl methacrylate) (PMMA) blended film on the elastomer substrate and evaluate the characteristics of the fabricated stretchable OTFTs. A field-effect mobility of 3.4 × 10−3 cm2 V−1 s−1, an Ion/Ioff ratio greater than 103, a subthreshold voltage swing of 12.5 V/decade, and a gate leakage current of 10−10 A were obtained. The endurable maximum strain was approximately 50% for the OTFTs without degrading the field-effect mobility formed on the elastomers.