gebhart/patrepeval huggingface dataset:
Wide Dynamic Range Doppler-Shift Compensation for Space-Borne Optical Communications (2004)

We model and demonstrate a novel concept for the Doppler-shift compensation of optical transmitters. Optical frequency offset locking is demonstrated using a simple Fabry-Perot laser diode transmitter and a frequency-lock loop. Our system simultaneously demonstrates a continuous tracking range of 21 GHz and a tracking speed of 44 THz/s. The transmitter frequency is adjusted over the entire 21-GHz range by merely adjusting the frequency of a microwave oscillator.
Evidence for communication between nerve growth factor and protein tyrosine phosphorylation (1990)

Nerve growth factor (NGF) stimulation of PC12 cells activated two myelin basic protein (MBP) kinase activities greater than 10-fold within 5 min, which were resolved by chromatography on Mono Q. Each enzyme phosphorylated MBP on threonine and was inactivated by incubation with either CD45, a protein tyrosine phosphatase, or protein phosphatase 2A (PP2A), a serine/threonine phosphatase. The effects of CD45 and PP2A were prevented by vanadate and okadaic acid, respectively. Activation of the MBP-kinases provides a mechanism for communication between NGF and intracellular protein tyrosine phosphorylation.
Titelbild: The Chemical Basis of Fungal Bioluminescence (Angew. Chem. 28/2015) (2015)

Leuchtende Pilze wurden von Aristoteles bereits im 4. Jahrhundert v. Chr. beschrieben. I. V. Yampolsky, J. I. Gitelson et al. decken in ihrer Zuschrift auf S. 8242 ff. die Struktur von Pilz-Luciferin auf, einer Verbindung, die für die Biolumineszenz von Pilzen verantwortlich ist. Leuchtende Pilze wurden von Aristoteles bereits im 4. Jahrhundert v. Chr. beschrieben. I. V. Yampolsky, J. I. Gitelson et al. decken in ihrer Zuschrift auf S. 8242 ff. die Struktur von Pilz-Luciferin auf, einer Verbindung, die für die Biolumineszenz von Pilzen verantwortlich ist. Krebsdiagnostik Ein Hypoxie erkennender Sauerstoffsensor für die Diagnose von Krebsmetastasierungen wird von X. Q. Jiang et al. auf S. 8212 ff. beschrieben. Nach systemischer Verabreichung des Sensors wird die Metastase von Krebszellen in die Lunge oder in Lymphknoten durch optische Ganzkörperbildgebung nachgewiesen.1 Elektroreduktion von NO Die entscheidende Rolle von Wasser für den Mechanismus der NO-Elektroreduktion auf Pt(111) untersuchen J. Greeley et al. in der Zuschrift auf S. 8373 ff. Wasser erleichtert den Elektronentransfer zu adsorbierten Oberflächenintermediaten mit sehr niedrigen kinetischen Barrieren.1 Porphyrinoide M. Bröring et al. präsentieren in ihrer Zuschrift auf S. 8331 ff. die erste radikalische freie Corrolbase. Der Verlust eines inneren H-Atoms des luftstabilen, leicht handhabbaren Porphyrinoids ergibt ein planares Molekül, das z. B. Zn2+ binden kann.1
A Tail Fiber Engineering Platform for Improved Bacterial Transduction-Based Diagnostic Reagents (2021)

Bacterial transduction particles were critical to early advances in molecular biology and are currently experiencing a resurgence in interest within the diagnostic and therapeutic fields. The difficulty of developing a robust and specific transduction reagent capable of delivering a genetic payload to the diversity of strains constituting a given bacterial species or genus is a major impediment to their expanded utility as commercial products. While recent advances in engineering the reactivity of these reagents have made them more attractive for product development, considerable improvements are still needed. Here, we demonstrate a synthetic biology platform derived from bacteriophage P1 as a chassis to target transduction reagents against four clinically prevalent species within the Enterobacterales order. Bacteriophage P1 requires only a single receptor binding protein to enable attachment and injection into a target bacterium. By engineering and screening particles displaying a diverse array of chimeric receptor binding proteins, we generated a potential transduction reagent for a future rapid phenotypic carbapenem-resistant Enterobacterales diagnostic assay.
Pharmacist-initiated management of antiretroviral therapy (PIMART) (2022)

We the undersigned, a collective of pharmacy&#13;\nresearchers, practitioners and academics, call on the Minister to&#13;\nconsider the following points in response to the input made by the&#13;\nSouth African Medical Association (SAMA) in relation to pharmacist-initiated management of antiretroviral therapy (PIMART), dated 9&#13;\nSeptember 2021. We wish to highlight the expertise of pharmacists&#13;\nin all matters relating to the design, production and use of quality,&#13;\nsafe and affordable medicines. Furthermore, we need to stress the&#13;\nincreasing importance of and role played by pharmacists in primary&#13;\ncare teams, which has been further reinforced during the COVID-19&#13;\npandemic and subsequent vaccination efforts.
PASOTRON{trademark} amplifier experiments (1995)

Preliminary experimental studies are reported of an L-Band amplifier based on Hughes` Plasma-Assisted, Slow-Wave Oscillator (PASOTRON) technology. The amplifier system utilizes a hollow-cathode-plasma electron-gun, and a plasma-filled Slow-Wave Structure (SWS) to produce {>=} 100-{micro}sec-long, 50 to 75-kV, 30 to 100-A electron-beam pulses that propagate in a plasma channel without the use of any externally applied axial magnetic field. The electron-beam pulse coincides with a 100-{micro}sec-long RF drive signal provided by a 2.6-kW TWT, which is coupled into the amplifier upstream of the SWS. The SWS consists of a ring-bar design which is novel to the PASOTRON family of devices and is used for its short length compared to a helix. Simulations on HP`s High Frequency Structure Simulator were used to optimize the ring-bar SWS. Preliminary data are reported showing the new L-Band amplifiers gain, power, efficiency, and bandwidth. Methods of eliminating a Backward Wave Oscillation (BWO), which was found to limit the performance of the tube, are also presented.
A new subband weight transform for delayless subband adaptive filtering structures (2005)

In applications like echo cancellation and speech enhancement, where there is need to track changes continuously, adaptive filtering is usually used. Long adaptive filters gives problems like slow convergence and high complexity. Subband adaptive filtering has been introduced to overcome these problems. The filter banks used in subband adaptive filtering introduce large delays. In order to compensate for the delays, delayless subband adaptive filtering is introduced. Delayless subband adaptive filtering is used in both open loop and closed loop configuration, where the subband filters are transformed to a fullband filter using a weight transform. This paper proposes a new subband weight transform based on the filter banks that are used. We investigate the performance of the weight transform using Monte Carlo simulations of a system identification situation. Different adaptive algorithms are used to compare the weight transform to previously proposed weight transforms.
68Ga-PSMA-11 PET Imaging of Response to Androgen Receptor Inhibition: First Human Experience (2016)

Inhibition of the AR can increase PSMA expression in prostate cancer metastases and increase the number of lesions visualized using PSMA PET. The effect seen in cell and animal models can be recapitulated in humans. A better understanding of the temporal changes in PSMA expression is needed to leverage this effect for both improved diagnosis and improved therapy.
Loop-mediated isothermal amplification (LAMP) assay for specific and rapid detection of Dickeya fangzhongdai targeting a unique genomic region (2022)

Dickeya fangzhongdai, a bacterial pathogen of taro (Colocasia esculenta), onion (Allium sp.), and several species in the orchid family (Orchidaceae) causes soft rot and bleeding canker diseases. No field-deployable diagnostic tool is available for specific detection of this pathogen in different plant tissues. Therefore, we developed a field-deployable loop-mediated isothermal amplification (LAMP) assay using a unique genomic region, present exclusively in D. fangzhongdai. Multiple genomes of D. fangzhongdai, and other species of Dickeya, Pectobacterium and unrelated genera were used for comparative genomic analyses to identify an exclusive and conserved target sequence from the major facilitator superfamily (MFS) transporter gene region. This gene region had broad detection capability for D. fangzhongdai and thus was used to design primers for endpoint PCR and LAMP assays. In-silico validation showed high specificity with D. fangzhongdai genome sequences available in the NCBI GenBank genome database as well as the in-house sequenced genome. The specificity of the LAMP assay was determined with 96 strains that included all Dickeya species and Pectobacterium species as well as other closely related genera and 5 hosts; no false positives or false negatives were detected. The detection limit of the assay was determined by performing four sensitivity assays with tenfold serially diluted purified genomic DNA of D. fangzhongdai with and without the presence of crude host extract (taro, orchid, and onion). The detection limit for all sensitivity assays was 100 fg (18-20 genome copies) with no negative interference by host crude extracts. The assays were performed by five independent operators (blind test) and on three instruments (Rotor-Gene, thermocycler and dry bath); the assay results were concordant. The assay consistently detected the target pathogen from artificially inoculated and naturally infected host samples. The developed assay is highly specific for D. fangzhongdai and has applications in routine diagnostics, phytosanitary and seed certification programs, and epidemiological studies.
Fast Monostatic Scattering Computation Based on Gaussian Beam Shooting and Frame Decomposition (2021)

Frame theory applied to source field decomposition facilitates the decomposition of an incident plane wave into a set of paraxial Gaussian beams. Spatial and spectral localization properties of such beams allow to bounce them through multiple reflections when reflecting surfaces are smooth and large enough as compared to wavelength. Gaussian beam spectral localization also yields dramatic reduction of the number of beams contributing to monostatic scattering cross section. An algorithm taking advantage of those properties is presented and applied to scattering by a set of blocks simulating an urban-like environment, with large dimensions as compared to wavelength. Numerical results will illustrate the method efficiency and will be compared to Fast Iterative Physical Optics results.
A case report of sevelamer-associated recto-sigmoid ulcers (2016)

Sevelamer is widely used in chronic kidney disease and end-stage renal disease patients with hyperphosphatemia. It is a crosslinked polymeric amine that binds phosphates and bile acids; it is not systemically absorbed. To the authors' knowledge, this is the first reported case of recto-sigmoid ulcers associated with use of this phosphate binder. Nephrologists, pathologists, and gastroenterology sub-specialists should be aware of this recently-reported entity in patients on sevelamer with suggestive symptoms, as this medication is widely used in renal patients.
Trapping of Syntaxin1a in Presynaptic Nanoclusters by a Clinically Relevant General Anesthetic (2018)

Propofol is the most commonly used general anesthetic in humans. Our understanding of its mechanism of action has focused on its capacity to potentiate inhibitory systems in the brain. However, it is unknown whether other neural mechanisms are involved in general anesthesia. Here, we demonstrate that the synaptic release machinery is also a target. Using single-particle tracking photoactivation localization microscopy, we show that clinically relevant concentrations of propofol and etomidate restrict syntaxin1A mobility on the plasma membrane, whereas non-anesthetic analogs produce the opposite effect and increase syntaxin1A mobility. Removing the interaction with the t-SNARE partner SNAP-25 abolishes propofol-induced syntaxin1A confinement, indicating that syntaxin1A and SNAP-25 together form an emergent drug target. Impaired syntaxin1A mobility and exocytosis under propofol are both rescued by co-expressing a truncated syntaxin1A construct that interacts with SNAP-25. Our results suggest that propofol interferes with a step in SNARE complex formation, resulting in non-functional syntaxin1A nanoclusters.
A parallel algorithm for state assignment of finite state machines (2002)

Optimization of huge sequential circuits has become unmanageable in CAD of VLSI due to enormous time and memory requirements. In this paper, we report a parallel algorithm for the state assignment problem for finite state machines. Our algorithm has three significant contributions: It is an asynchronous parallel algorithm portable across different MIMD machines. Time and memory requirements reduce by a factor of P (the number of processors), enabling it to handle large problem sizes which the sequential algorithm fails to handle. The quality of the results for multiprocessor runs remains comparable to the sequential algorithm on which it is based.
Compressed Video Over Networks (2018)

Video: fundamentals of video compression - H.263 as an example MPEG digital video coding standards. Networks: IP networks ATM networks wireless systems and networking. Systems for video networking: error concealment for video communication layered coding error resilience coding variable bit rate video coding. Transport of compressed video: Internet video wireless video ITU-T and DAVIC systems standards.
Protocol for the Mason: Health Starts Here prospective cohort study of young adult college students (2021)

The study will produce new knowledge to help understand the development of health-related behaviors during young adulthood. A long-term goal of the cohort study is to support the design of effective, low-cost interventions to encourage young adults' consistent performance of healthful behaviors, improve their mental health, and improve academic performance.
Genomic Localization of the Human Gene for KCNA10, a cGMP-Activated K Channel (1997)

Potassium (K) channels are important components of virtually all cells, and they play critical roles in many cellular functions. KCNA10 represents a new class of K channel specifically regulated by cGMP and postulated to mediate the effects of substances that increase intracellular cGMP. Since KCNA10 has the potential to be useful in candidate gene analysis of inherited diseases, the human gene for KCNA10 was characterized. Fluorescence in situ hybridization indicates that human KCNA10 maps to chromosome 1 at p13.1-->p22.1. Finer mapping of the gene was achieved by PCR of a set of CEPH YAC clones that spanned the region of interest. We found that YAC 818b9 contains human KCNA10. These data indicate human KCNA10 maps to 1p13.1 and resides within the genetic interval defined by microsatellite loci D1S2809 and D1S2726. That region of chromosome 1 contains another K channel gene, KCNA3.
Toward a Theory of Professional Diagnosis and Service: Consumer Behavior (1980)

A model is developed for situations in which consumers depend upon producers of a good or service for information which has an impact on their demand. Nonsupply sources of information do not exist and consumers are forced to rely on comparisons between suppliers as their only check on potential fraud. The optimal search strategies are characterized and some of the implications for the resulting patterns of advice are analyzed.
Artemia salina as a Test Organism for Bioassay (1956)

One of the series of "Yale natural radiocarbon measurements, II" [Science 122, 954 (1955)], by Richard S. Preston, Elaine Person, and E. S. Deevey, was incorrectly reported through a technical error. The date for sample Y-293A (South Haven, Mich.: peat) was 10,790 +/- 200 yr, not 9500 + 250 yr as reported on page 958.
Elementi di crittografia (2015)

Dagli antichi intrighi diplomatici all'enorme mole di messaggi che si scambiano oggi in forma elettronica, la crittografia ha svolto un ruolo cruciale nella storia divenendo una disciplina critica e complessa. Scopo di questo volume è darne una presentazione che associ il rigore matematico a una ragionevole semplicità di comprensione.&#13;\nIl testo è nato nell'ambito dell'insegnamento universitario ma è destinato anche a coloro che operano nel campo dell'informatica, che potranno apprendervi come funzionano i sistemi crittografici e cosa ci si possa legittimamente attendere da essi. E, perché no, anche ai curiosi&#13;\ndi matematica cui la crittografia di oggi è indissolubilmente legata.&#13;\nPoiché è impossibile affrontare gli studi di crittografia senza passare attraverso l'algoritmica e l'algebra, il testo dedica una parte ai concetti di base di queste discipline per poi presentare i cifrari attuali, i loro prevedibili sviluppi, i problemi di riservatezza e protezione dei dati, le comunicazioni su Internet, le applicazioni in campo finanziario; e si conclude con una proiezione sul futuro in relazione al calcolo quantistico.
Prenatal disruption of blood–brain barrier formation via cyclooxygenase activation leads to lifelong brain inflammation (2022)

Gestational maternal immune activation (MIA) in mice induces persistent brain microglial activation and a range of neuropathologies in the adult offspring. Although long-term phenotypes are well documented, how MIA in utero leads to persistent brain inflammation is not well understood. Here, we found that offspring of mothers treated with polyriboinosinic–polyribocytidylic acid [poly(I:C)] to induce MIA at gestational day 13 exhibit blood–brain barrier (BBB) dysfunction throughout life. Live MRI in utero revealed fetal BBB hyperpermeability 2 d after MIA. Decreased pericyte–endothelium coupling in cerebral blood vessels and increased microglial activation were found in fetal and 1- and 6-mo-old offspring brains. The long-lasting disruptions result from abnormal prenatal BBB formation, driven by increased proliferation of cyclooxygenase-2 (COX2; Ptgs2)-expressing microglia in fetal brain parenchyma and perivascular spaces. Targeted deletion of the Ptgs2 gene in fetal myeloid cells or treatment with the inhibitor celecoxib 24 h after immune activation prevented microglial proliferation and disruption of BBB formation and function, showing that prenatal COX2 activation is a causal pathway of MIA effects. Thus, gestational MIA disrupts fetal BBB formation, inducing persistent BBB dysfunction, which promotes microglial overactivation and behavioral alterations across the offspring life span. Taken together, the data suggest that gestational MIA disruption of BBB formation could be an etiological contributor to neuropsychiatric disorders.
Supplementary Figure 8 from Molecular Photoacoustic Imaging of Follicular Thyroid Carcinoma (2023)

&lt;p&gt;PDF file - 866K, Photoacoustic and optical imaging of the probe cleavage in tumors with different levels of MMPs: HT1080 (high MMP) and BT20 (low MMP)&lt;/p&gt;
Effects of adenosine 3′5′‐cyclic monophosphoric acid on the morphology, growth and cell cycle of earle's L cells (1974)

Abstract The morphology of Earle's L cells is radically altered after incubation with adenosine 3′5′‐cyclic monophosphate (cyclic AMP) and cell growth is inhibited. The concentration of nucleotide required to completely inhibit cell growth depends on the initial cell density although the breakdown of cyclic AMP in the supernatant fluid to adenosine is independent of the cell density. A decrease in mitotic index is noted several hours after the addition of cyclic AMP, while a reduction in the fraction of cells in S is only observed after 2 days. Upon removal of the drug an increase in the number of cells synthetizing DNA (as detected autoradiographically) is observed well before the increase of mitotic rate. These results suggest that cells are blocked in moving through S to G2. Cells resume normal growth rates and morphology only 6–8 days after the removal of cyclic AMP, but can then be propagated indefinitely.
トロンボーン奏者ならだれでも知っておきたい「からだ」のこと (2012)

第１章 はじめに 第２章 ボディ・マップと筋感覚 第３章 ボディ・マッピング 第４章 包括的認識力と建設的な休息 第５章 脊柱 第６章 バランス 第７章 呼吸 第８章 アンブシュア 第９章 腕 第１０章 脚 第１１章 探求
772 Long-Term Mucosal Healing, Clinical Response and Clinical Remission in Patients With Ulcerative Colitis Treated With the Anti-MAdCAM-1 Antibody Ontamalimab: Results From the Open-Label Extension Study TURANDOT II (2019)

INTRODUCTION: Ontamalimab (SHP647), a human monoclonal IgG 2 antibody, targets mucosal addressin cell adhesion molecule-1 (MAdCAM-1), to reduce lymphocyte homing to the gastrointestinal (GI) tract. In the TURANDOT II trial, ontamalimab was well-tolerated and clinical benefit was seen up to 144 wks in patients with ulcerative colitis (UC). Here we report long-term mucosal healing, response and remission in a subset of patients in TURANDOT II. METHODS: TURANDOT II (NCT01771809) is a phase 2, 2-part open-label (OL) extension study of ontamalimab in patients with UC who received placebo or ontamalimab 7.5, 22.5, 75 or 225 mg in the feeder study (TURANDOT). At TURANDOT II baseline (TURANDOT wk 12), patients were randomized to ontamalimab 75 or 225 mg every 4 wks for 72 wks (OL1). In cases of clinical exacerbation or no response, escalation from 75 to 225 mg was permitted from wk 8. In OL2, patients received 75 mg every 4 wks for 72 wks. Endoscopies were carried out at wk 16, and a subset of patients undergoing routine cancer surveillance had at least one follow-up endoscopy between wks 40 and 72 of OL1: all endoscopies were centrally-read. Mucosal healing (Mayo endoscopy subscore ≤1), clinical remission (total Mayo score ≤2, no subscore &gt;1) and clinical response (≥3-point decrease in total Mayo score from TURANDOT baseline, ≥30% change; ≥1-point decrease in or ≤1 rectal bleed absolute score) were measured. RESULTS: Of 330 patients in TURANDOT II, 101 had follow-up endoscopies (Table 1), 25.7% (n = 26) of whom had mucosal healing at TURANDOT II baseline. At wk 16, 43.6% (n = 44) had mucosal healing, and 33 of these patients (75%) maintained mucosal healing up to wk 40–72. Of 65 responders in TURANDOT, 37 (56.9%) had mucosal healing at both wk 16 and wk 40–72. Of 36 non-responders, 7 (19.4%) and 9 (25%) achieved mucosal healing at wk 16 and wk 40–72, respectively (Figure 1). Of the responders, 58 (89.2%) maintained response and 27 (41.5%) were in remission at wk 16; at wk 40–72, 53 (81.5%) maintained response and 33 (50.8%) were in remission. Of non-responders, 22 (61.1%) achieved response and 5 (13.9%) achieved remission by wk 16; by wk 40–72, 17 (47.2%) had responded and 8 (22.2%) were in remission. Overall, the mean Mayo endoscopic subscore of 2 (SD, 0.1) was maintained to wk 40–72. CONCLUSION: Mucosal healing, response and remission persisted in a subset of patients who continued ontamalimab treatment up to 72 wks and underwent surveillance endoscopies between wks 40 and 72.
Impact of 3′-Exonuclease Stereoselectivity on the Kinetics of Phosphorothioate Oligonucleotide Metabolism (1998)

For the enzymatic digestion of a 25-mer phosphorothioate (PS) oligonucleotide, the reaction kinetics was previously determined to be the sum of two parallel processes: a fast and a very slow phase of digestion suggesting a two-exponential model. A characteristic metabolite profile was observed both in vitro and in vivo. This behavior is shown to be the result of the stereoselective cleavage of chiral R-configuration and S-configuration PS internucleotide linkages by 3'-exonucleases. The stereoselective nature of 3'-exonuclease action was analyzed by reverse-phase HPLC. The separation of eight diastereomers of the tetramer TTCT (5'-3') was used to follow the stereoselective course of exonuclease hydrolysis of PS internucleotide linkages. Degradation of the 25-mer parent compound having a 3' S-terminal internucleotide linkage was calculated to be more than 300 times slower than an analog with a 3'-terminal R-configuration. These results support an approach for protecting antisense oligonucleotides based on the chirality of only the 3'-end internucleotide linkage.