gebhart/patrepeval huggingface dataset:
An integrated approach for product family and assembly system design (2003)

The main objective of the research presented in this paper was to develop a new philosophy for the design of a product family (PF) and its assembly system. Most methodologies usually do not consider the interactions between these two design processes. Moreover, most "assembly-centered" concurrent engineering approaches do not deal with PFs. A research project, called CISAL, was initiated in Belgium in the late nineties to tackle this problem. The proposed tool is intended to be applicable to PFs and hybrid assembly lines (presenting manual, robotic and automated operations). It is subdivided into three interacting main modules: preliminary design for assembly (DFA) and assembly planning (AP); detailed DFA and AP; and line layout. The whole methodology is articulated around the functional entities (FEns), resulting from a first-order decomposition of the PF and aiming at reducing the complexity inherent to the design of multivariant products. All tools presented take this decomposition into account and treat design issues related to each FEn separately, considering the PF as an assembly of FEns. This paper presents the framework of CISAL, and the underlying methodology is illustrated on an industrial case study.
Low temperature protonation of cyclo-octa-1,5-diene-(duroquinone)nickel(0) (1974)

Cyclo-octa-1,5-diene(duroquinone)nickel(O) has been shown to protonate at the duroquinone carbonyls in HSO3F to yield a stable dication. The chemical shift of the OH protons indicates very extensive positive charge delocalization through backbonding.
Sulfur Betaines from S-Propargyl Xanthates. Unusual Chemistry from a Simple Functional Group (2019)

S-Propargyl xanthates undergo upon heating a [3,3] sigmatropic rearrangement followed by a reversible ring closure into a novel betaine. This betaine can be implicated in carbon–carbon bond forming processes, in the synthesis of esters, in the inversion of secondary alcohols, in the formation of alkenes, for the generation of rigid, cisoid dienes that are highly reactive in both inter- and intra-molecular Diels–Alder cycloadditions, and in various other synthetically useful transformations. 1 Introduction 2 An Unexpected Transformation 3 Evidence for the Betaine Intermediate 4 A Method for the Synthesis of Esters and for the Inversion of Secondary Alcohols 5 A General Alkylation Process 6 The Case of Carbon Acids 7 A Synthesis of Alkenes 8 Further Trapping Experiments. Concerted or Not Concerted? 9 Rigid Cisoid Dienes 10 Propargyl Radicals 11 Concluding Remarks
Two-photon imaging of endogenous biomolecules in freshly excised human cervical tissue biopsies captures pre-cancer induced changes in tissue metabolism and morphology (2023)

Fiber endoscopes capable of making two-photon (2P) autofluorescence measurements, time-resolved fluorescence decay measurements, and collagen second harmonic generation (SHG) measurements have been applied to animal models. Clinical translation of such devices to internal human organs has the potential to overhaul conventional methods of disease diagnosis and monitoring. Previous work by our lab has established the potential to diagnose high-grade cervical precancers using 2P autofluorescence measurements. Other groups have demonstrated that 2P-based fluorescence lifetime imaging microscopy (FLIM) measurements of nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and collagen SHG measurements have the potential to discriminate between cancerous and benign tissues. In this work, we demonstrate the potential to discern high-grade cervical precancerous lesions (HSILs) from benign tissues using fluorescence intensity measurements of NAD(P)H and oxidized flavoproteins, FLIM NAD(P)H measurements, and collagen SHG measurements. Consistent with previous results, benign tissues demonstrated increased depth-dependent heterogeneity in mitochondrial clustering, and increased overall and intrafield heterogeneity of oxido-reductive state relative to HSILs. FLIM phasor analysis demonstrated a relative decrease in NAD(P)H short and long lifetime, and a relative increase in NAD(P)H bound fraction for benign tissues compared to HSILs. Collagen SHG intensity in benign tissues was greater than that of HSIL tissues, along with overall intrafield variations in collagen fiber orientation. This work motivates the functionalization of a clinical 2P fiber endoscope capable of making SHG, autofluorescence intensity and lifetime measurements of metabolic coenzymes in the human cervix.
Supplementary Figure 3 from Potent <i>In vitro</i> and <i>In vivo</i> Activity of an Fc-Engineered Anti-CD19 Monoclonal Antibody against Lymphoma and Leukemia (2023)

Supplementary Figure 3 from Potent &lt;i&gt;In vitro&lt;/i&gt; and &lt;i&gt;In vivo&lt;/i&gt; Activity of an Fc-Engineered Anti-CD19 Monoclonal Antibody against Lymphoma and Leukemia
PAK1 Kinase Promotes Cell Motility and Invasiveness through CRK-II Serine Phosphorylation in Non-Small Cell Lung Cancer Cells (2012)

The role of c-Crk (CRK) in promoting metastasis is well described however the role of CRK phosphorylation and the corresponding signaling events are not well explained. We have observed CRK-II serine 41 phosphorylation is inversely correlated with p120-catenin and E-cadherin expressions in non-small cell lung cancer (NSCLC) cells. Therefore, we investigated the role of CRK-II serine 41 phosphorylation in the down-regulation of p120-catenin, cell motility and cell invasiveness in NSCLC cells. For this purpose, we expressed phosphomimetic and phosphodeficient CRK-II serine 41 mutants in NSCLC cells. NSCLC cells expressing phosphomimetic CRK-II seine 41 mutant showed lower p120-catenin level while CRK-II seine 41 phosphodeficient mutant expression resulted in higher p120-catenin. In addition, A549 cells expressing CRK-II serine 41 phosphomimetic mutant demonstrated more aggressive behavior in wound healing and invasion assays and, on the contrary, expression of phosphodeficient CRK-II serine 41 mutant in A549 cells resulted in reduced cell motility and invasiveness. We also provide evidence that PAK1 mediates CRK-II serine 41 phosphorylation. RNAi mediated silencing of PAK1 increased p120-catenin level in A549 and H157 cells. Furthermore, PAK1 silencing decreased cell motility and invasiveness in A549 cells. These effects were abrogated in A549 cells expressing phosphomimetic CRK-II serine 41. In summary, these data provide evidence for the role of PAK1 in the promotion of cell motility, cell invasiveness and the down regulation of p120-catenin through CRK serine 41 phosphorylation in NSCLC cells.
DNPLab/DNPLab: DNPLab 1.0.8 (2021)

Fix issue with importing winEPR parameters Removing HydrationGUI Adding polyfit function
Mice lacking IgA exhibit defective IgG antibody responses to polysaccharide conjugate vaccine (84.14) (2010)

Abstract Streptococcus pneumoniae is a leading cause of global disease. While capsular polysaccharide conjugate vaccines have decreased disease burden; a portion of the population remains unresponsive to vaccination. In order to understand the mechanisms responsible vaccine non-responsiveness, studies have been performed using mice with a targeted gene deletion that causes lack of IgA but few alterations in expression of other Ig isotypes. Surprisingly, IgA-/- mice were found nonetheless to exhibit specific defects in both IgA and IgG antibody responses to the polysaccharide (PS) component of pneumococcal conjugate vaccine. Neither IgM anti-PS responses nor IgG antibody responses to the protein component of the conjugate vaccine were affected. Furthermore, IgA-/- mice expressed fewer peritoneal B1a cells. As B1a and marginal zone B cells are considered to be the primary cell types that respond to polysaccharide antigens, this might to some extent explain the decreased anti-PS serum antibody titers seen in IgA-/- mice. Studies using bone marrow chimeras showed that reconstitution of IgA-/- mice with IgA+/+ B cells failed to restore antibody responsiveness to the PS component of the conjugate vaccine. Taken together, these results indicate that a defect in IgA expression affects B cell homeostasis, which is partially influenced by the environment that is present during B cell activation. (Supported by NIH grants RO1 41715 and R21 83878).
In vivo isolation of circulating tumor cells in non-small cell lung cancer (NSCLC) patients by a medical device. (2012)

10536 Background: Currently, circulating tumor cells (CTCs) are isolated in vitro from small limited volumes of blood samples. Furthermore, CTC results for NSCLC as a prognostic and stratification biomarker are scarce. The aim of the study was to assess a functionalized and structured medical wire (FSMW) for in vivo isolation of CTCs directly from the blood of NSCLC patients, and to compare it to the Cell Search method. Methods: The device was inserted in a cubital vein through a standard cannula for thirty minutes. The interaction of target CTCs with the FSMW was mediated by an antibody directed against the epithelial cell adhesion molecule (EpCAM). To confirm the CTCs binding to the wire, the immunohistochemical staining against EpCAM as well as CD45 for negative cell selection was performed. There were 72 applications of the wire in 48 stage I-IIIB NSCLC patients (12 double applications) and 12 non cancer patients. Enumeration data was available for 34 NSCLC patients and 8 non cancer patients. For 34 patients, samples were also tested in the Cell Search system. Results: The device was well tolerated in all 72 applications without side effects. We obtained in vivo isolation of CTCs in 32 of 34 NSCLC patients (94.1%) with a median (range) of 13 (0-300) CTCs. The sensitivity was similar for early and late stage NSCLC patients. In the non-cancer patients, no CTCs were detected (100 % specificity). In 2 of 34 samples (5.8%), CTCs were detected by the Cell Search method. In all matched pairs, the FSMW detected the same number or more CTCs compared to the Cell Search method. Conclusions: Whilst well tolerated without side effects, the CTC detection rate of the FSMW in NSCLC patients was &gt;90%. In contrast, &lt;10% detection was obtained using the Cell Search analysis. No CTCs were detected in non-cancer patients. This proof of concept study may have important clinical implications, as the implementation of the device into clinical practice may improve early detection, prognosis and therapy monitoring of NSCLC patients. The method may also allow the molecular analysis of the CTCs, with the possibility of establishing more personalized treatment regiments.
Study on cell culture processing system to improve task efficiency (System concept and efficiency improvement of the discarding task using a robot in a media change process) (2018)

In the field of regenerative medicine, cell processing operation mainly depends on manpower. Moreover, it requires a large amount of labor and cost, and its efficiency needs to be improved. Automatic cell culture apparatuses equipped with a vertical articulated robot have recently been proposed. However, automating all tasks of cell processing complicates the system constitution. This study aims to develop a simple and rational cell processing system by combining the tasks performed by a robot and a human. Herein, we first analyze each task in the cell processing operation, verify whether a task can be efficiently performed by a robot and automated equipment, and judge sharing with a human. In this study, we examined the improvement in the efficiency of the discarding task of spent culture media in a flask using a robot arm in a media change process. We conducted task analysis on the discarding task performed by a cell processing expert and beginner and conducted experiments using a robot on the discarding task based on the analysis results. We proposed an optimum condition of the discarding task considering the dripping risk owing to the characteristics of the culture media and showed that a robot could perform the discarding task more efficiently than a human; the dripping risk could also be reduced using a robot.
CHAPTER 9. PLA-nanocellulose Biocomposites (2014)

Bio-reinforced green composites from biodegradable polymers and natural fibres have received increasing scientific and industrial attention in the fields of packaging, medical and automotive sectors. Poly(lactic acid) (PLA) is one of the most promising biodegradable thermoplastic polyesters with good mechanical properties. However, the insufficient impact strength, inherent brittleness and low thermal stability of PLA limit the scope of its applications. Considerable efforts have been made to improve its properties in order to achieve compatibility with thermoplastics processing and manufacturing, and compete with commodity polymers. Cellulose nanofibrils with lateral dimensions three orders of magnitude smaller than the intact plant fibre cells are an ideal reinforcing material for nanocomposites due to high aspect ratio, large specific surface area and high mechanical strength/weight performance. In this chapter, we review the preparation, structure and properties of PLA/nanocellulose biocomposites with particular focus on the effect of surface modification on nanocellulose dispersion, and the effect of nanocellulose incorporation on crystallization kinetics of PLA matrix and mechanical, thermal and barrier properties of biocomposites.
Therapeutic Cocktail Approach for Treatment of Hyperhomocysteinemia in Alzheimer’s Disease (2018)

In the United States, Alzheimer's disease (AD) is the most common cause of dementia, accompanied by substantial economic and emotional costs. During 2015, more than 15 million family members who provided care to AD patients had an estimated total cost of 221 billion dollars. Recent studies have shown that elevated total plasma levels of homocysteine (tHcy), a condition known as hyperhomocysteinemia (HHcy), is a risk factor for AD. HHcy is associated with cognitive decline, brain atrophy, and dementia; enhances the vulnerability of neurons to oxidative injury; and damages the blood-brain barrier. Many therapeutic supplements containing vitamin B12 and folate have been studied to help decrease tHcy to a certain degree. However, a therapeutic cocktail approach with 5-methyltetrahydrofolate, methyl B12, betaine, and <i>N</i>-acetylcysteine (NAC) have not been studied. This novel approach may help target multiple pathways simultaneously to decrease tHcy and its toxicity substantially.
Viruses Contained in Droplets Applied on Warmed Surface Are Rapidly Inactivated (2014)

Heat inactivation of viruses was reported, however, the thermal resistance of viruses in droplets has not been studied. The aim of this study was to evaluate the pattern of heat resistance of minute virus of mice (MVM), coxsackievirus B4 (CVB4), influenza A virus (H1N1), and herpes simplex virus type 1 (HSV1) contained in droplets. Four μL droplets containing viruses (> 10(4.5) TCID50) were applied onto warmed surface obtained by using a self-made heating device. Viral suspensions were exposed to temperatures ranging from 70 to 130°C for 0 to 90 min depending on the virus, and then the recovered viral preparations were tittered. Inactivation rates were calculated from curves that were analysed according to the first order kinetics model. Full inactivation was obtained for MVM in 90 min at 80°C and in 2 s at 130°C, for H1N1 in 14 s at 70°C and in 1 s at 110°C, for CVB4 and HSV-1 in 5 s and 7 s respectively at 70°C and in 1 s at 100°C. Clearly, MVM was more resistant than H1N1 that was more resistant than HSV-1 and CVB4, which was reflected by increasing inactivation rates. The impact of short time exposure to heat onto the infectivity of viruses contained in a small volume of suspension has been determined. For the first time, the inactivation of viral particles contained in drops exposed to temperatures higher than 100°C has been investigated. It appears that heating can have an unexpected faster virucidal effect than previously described.
Somatostatin, gastrointestinal peptides, and the carcinoid syndrome. (1981)

During a control infusion noradrenaline and alcohol each provoked carcinoid flushing in four of five patients and pentagastrin in two of five patients. When tetradecapeptide somatostatin was infused on another day no patient flushed at any time, even when 16 microgram of either noradrenaline or pentagastrin were administered. Carcinoid flushing was not associated with release of gastrin or any of the other vasoactive or postprandially released gut regulatory peptides measured. In a sixth patient with severe prolonged carcinoid flushing, subcutaneous Des AA1, 2, 4, 5, 12D Trp8 somatostatin markedly reduced the incidence and severity of flushing for two days. Somatostatin is thus a potent inhibitor of carcinoid flushing, but no evidence has been found for the gut hormones measured to be mediators of flushing.
A Dynamic Gesture Prediction System Based on the CLCS Feature Extraction (2013)

Real-time recognition of dynamic gestures is a problem for most of the applications nowadays. The prediction approach can be used as a solution for this. This approach uses an incomplete gesture input and it tries to predict which gesture the given input represents. This paper presents the application of the dynamic gesture feature extraction technique called Convexity Local Contour Sequence (CLCS) as the extractor for the prediction task. Two predictor systems are used to achieve this task and results are compared and discussed in this paper.
Near-field infrared microscopy with a transient photoinduced aperture (2001)

We report a method of near-field infrared microscopy with a transient optically induced probe. Photoinduced reflectivity in semiconductors is used to generate a relatively large transient mirror with a small aperture (infrared probe) in its center. Properties of this probe have been studied and first images obtained using the technique are presented. Resolution better than λ/5 at 6.25 μm is demonstrated. Among the advantages of this technique are high optical throughput of the probe, ease in simultaneous visible imaging, and a high scanning rate limited primarily by the pulse repetition rate of the laser system.
A New Lyotropic Liquid Crystalline System: Oligo(ethylene oxide) Surfactants with [M(H2O)n]Xm Transition Metal Complexes (2001)

Koordinierte Wassermoleküle induzieren die Aggregation und Selbstorganisation einer lyotropen flüssigkristallinen Phase, die aus nichtionischen Tensiden CnH2n+1(CH2CH2O)mOH und Übergangsmetall-Wasser-Komplexen ([Ni(H2O)6](NO3)2, [Co(H2O)6](NO3)2, [Zn(H2O)6](NO3)2, [Cd(H2O)4](NO3)2 und [Co(H2O)6]Cl2) gebildet wird, sodass hexagonale (siehe schematische Darstellung) und/oder kubische Strukturen entstehen. NiII- und CoII-Komplexe rekristallisieren und zeigen Phasentrennung bei hohen Komplex-Konzentrationen, während ZnII- und CdII-Komplexe bei einem Metall/Tensid-Verhältnis größer als 3.2:1 (Raumtemperatur) kubische Phasen bilden.
A procedure to verify an amino acid sequence which has been derived from a nucleotide sequence: application to the 26S RNA of Semliki Forest virus (1982)

We describe a peptide sequencing procedure which can be used to verify an amino acid sequence which is derived from a nucleotide sequence. One first labels the protein with a 3H- and a 14C-labelled amino acid and then cleaves the protein into a set of peptides using a cleavage reaction specific for a particular amino acid residue. Finally one performs Edman degradations on the whole mixture of peptides. The released amino acids reflect the combined aminoterminal amino acid sequences of all the peptides that have been formed by the cleavage reaction. The data can therefore be used to check a deduced sequence simultaneously at several regions of the polypeptide chain. We have applied this sequencing procedure to verify the amino acid sequence deduced from the 26S RNA of Semliki Forest virus.
Incorporation of DOPA by amelanotic melanoma cells: A comparison of data from patient biopsy, soft bilayer agar assay, and xenograft (1982)

The distinction between metastatic amelanotic melanoma\nand a poorly differentiated carcinoma is often difficult.\nWith the use of the ultrastructural L-DOPA reaction as a\ntool for measuring tyrosinase activity within vesicular and\ncisternal organelles, cells containing melanin can be\nidentified. Our study identified a tumor which was classified\nby light microscopy as an undifferentiated carcinoma\nand by electron microscopy as an undifferentiated neoplasm.\nNine parameters were evaluated: a nude mouse xenograft,\nthe patient's tumor cells in agar with and without L-DOPA\ntreatment, and single cell suspensions of the patient's\ntumor cells with and without L-DOPA treatment. As controls,\ncells from Cloudman S91 (CCL) 53.1 melanoma, grown into\ncolonies in agar with and without L-DOPA treatment and in\nsingle cell suspensions with and without L-DOPA treatment,\nwere evaluated. The xenograft demonstrates no melanosomes.\nCells in single cell suspensions with L-DOPA treatment and\npatient cells grown into colonies in agar with and without\nL-DOPA treatment contain granular dense bodies within the\ncytoplasm, suggestive of melanosomes. Premelanosomes (Stage\nII), Golgi, and Golgi-associated smooth endoplasmic reticulum\ncontain DOPA-positive reaction product when cultured in\nthe agar assay. A DOPA-positive reaction is also noted in\nthe Golgi-associated smooth endoplasmic reticulum of the\nsingle cell suspensions. The CCL line demonstrates premelanosomes\nin all cases. These. findings unequivocally\nidentify the tumor as amelanotic melanoma and indicate that\nidentification of DOPA-positive cells grown into colonies\nin agar are essential for diagnosis. These parameters may\nbe applied to ambiguous cases of malignancy for which\namelanotic melanoma is suspected. (Supported in part by\ngrants from NIH (T32 CA09213 to B.P.) and ACS (PDT-184 to\nF.L.M. and PDT-205 to M.J.C.H.)
Endoscopic forward-viewing optical coherence tomography and angiography with MHz swept source (2017)

Endoscopic optical coherence tomography (OCT) instruments are mostly side viewing and rely on at least one proximal scan, thus limiting accuracy of volumetric imaging and en face visualization. Previous forward-viewing OCT devices had limited axial scan speeds. We report a forward-viewing fiber scanning 3D-OCT probe with 900 Âµm field of view and 5 Âµm transverse resolution, imaging at 1 MHz axial scan rate in the human gastrointestinal tract. The probe is 3.3 mm diameter and 20 mm rigid length, thus enabling passage through the endoscopic channel. The scanner has 1.8 kHz resonant frequency, and each volumetric acquisition takes 0.17 s with 2 volumes/s display. 3D-OCT and angiography imaging of the colon was performed during surveillance colonoscopy.
Anti-cytokine strategies. (1992)

Although antibiotics and support systems have reduced the mortality due to bacterial infections, 40-50% mortality is still an unacceptable statistic. Anti-LPS (anti-lipid A) passive immunotherapy has reduced this mortality but only in patients with documented Gram-negative bacteriemia. However, anti-cytokine therapy such as monoclonal antibodies to TNF and the IL-1ra have reduced mortality to all infectious causes. IL-1ra is presently in Phase III trials as is monoclonal anti-TNF. Two other strategies are soluble (extracellular domains) receptors to TNF and IL-1. These are now entering Phase I trials but animal data strongly support that similar to antibodies to TNF and IL-1ra, these anti-cytokine therapies will also be effective.
Closed system processing variables affect post‐thaw quality characteristics of cryopreserved red cell concentrates (2022)

When manufacturing cryopreserved RCCs using the ACP 215, dual centrifugation processing with AS-3 or ESOL additive solutions is preferred, with storage periods of up to 21 days both pre-processing and post-deglycerolization.