gebhart/patrepeval huggingface dataset:
RECQL, a Member of the RecQ Family of DNA Helicases, Suppresses Chromosomal Instability (2006)

The mouse gene Recql is a member of the RecQ subfamily of DEx-H-containing DNA helicases. Five members of this family have been identified in both humans and mice, and mutations in three of these, BLM, WRN, and RECQL4, are associated with human diseases and a cellular phenotype that includes genomic instability. To date, no human disease has been associated with mutations in RECQL and no cellular phenotype has been associated with its deficiency. To gain insight into the physiological function of RECQL, we disrupted Recql in mice. RECQL-deficient mice did not exhibit any apparent phenotypic differences compared to wild-type mice. Cytogenetic analyses of embryonic fibroblasts from the RECQL-deficient mice revealed aneuploidy, spontaneous chromosomal breakage, and frequent translocation events. In addition, the RECQL-deficient cells were hypersensitive to ionizing radiation, exhibited an increased load of DNA damage, and displayed elevated spontaneous sister chromatid exchanges. These results provide evidence that RECQL has a unique cellular role in the DNA repair processes required for genomic integrity. Genetic background, functional redundancy, and perhaps other factors may protect the unstressed mouse from the types of abnormalities that might be expected from the severe chromosomal aberrations detected at the cellular level.
A Mouse Model for Conditional Secretion of Specific Single-Chain Antibodies Provides Genetic Evidence for Regulation of Cortical Plasticity by a Non-cell Autonomous Homeoprotein Transcription Factor (2016)

During postnatal life the cerebral cortex passes through critical periods of plasticity allowing its physiological adaptation to the environment. In the visual cortex, critical period onset and closure are influenced by the non-cell autonomous activity of the Otx2 homeoprotein transcription factor, which regulates the maturation of parvalbumin-expressing inhibitory interneurons (PV cells). In adult mice, the maintenance of a non-plastic adult state requires continuous Otx2 import by PV cells. An important source of extra-cortical Otx2 is the choroid plexus, which secretes Otx2 into the cerebrospinal fluid. Otx2 secretion and internalization requires two small peptidic domains that are part of the DNA-binding domain. Thus, mutating these "transfer" sequences also modifies cell autonomous transcription, precluding this approach to obtain a cell autonomous-only mouse. Here, we develop a mouse model with inducible secretion of an anti-Otx2 single-chain antibody to trap Otx2 in the extracellular milieu. Postnatal secretion of this single-chain antibody by PV cells delays PV maturation and reduces plasticity gene expression. Induced adult expression of this single-chain antibody in cerebrospinal fluid decreases Otx2 internalization by PV cells, strongly induces plasticity gene expression and reopens physiological plasticity. We provide the first mammalian genetic evidence for a signaling mechanism involving intercellular transfer of a homeoprotein transcription factor. Our single-chain antibody mouse model is a valid strategy for extracellular neutralization that could be applied to other homeoproteins and signaling molecules within and beyond the nervous system.
Autoantibodies to the IGF1 Receptor in Graves' Orbitopathy (2012)

Our data demonstrate the existence of IGF1R-Abs in humans but do not support the hypothesis that the IGF1R-Abs contribute to GO pathogenesis.
Annulative ring expansions. Direct conversion of .omega.-alkynyl acetals to polycyclic unsaturated ketones (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAnnulative ring expansions. Direct conversion of .omega.-alkynyl acetals to polycyclic unsaturated ketonesJoseph Sisko, Aaron Balog, and Dennis P. CurranCite this: J. Org. Chem. 1992, 57, 16, 4341–4342Publication Date (Print):July 1, 1992Publication History Published online1 May 2002Published inissue 1 July 1992https://doi.org/10.1021/jo00042a007RIGHTS & PERMISSIONSArticle Views250Altmetric-Citations17LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (235 KB) Get e-AlertsSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
CNS oxygen toxicity in the rat: role of ambient illumination. (1986)

The effect of ambient illumination on sensitivity to CNS oxygen toxicity was studied in awake male rats. Continuous recording of the EEG was obtained with chronically implanted cortical electrodes. The appearance of the electrical discharges in the EEG was used as an end point for CNS oxygen toxicity. It was found that sensitivity to CNS oxygen toxicity was inversely related to the level of ambient light illumination. The latent period for appearance of the electrical discharges was significantly shorter in darkness than in light over most of the oxygen pressures. Rats with severely depressed retinal function were as sensitive to oxygen at high pressure as normal rats in darkness, demonstrating the importance of visual input in the modulation of sensitivity to CNS oxygen toxicity.
Antimycotic-Antibiotic Amphotericin B Promotes Influenza Virus Replication in Cell Culture (2011)

In general, antibiotics are not rated as substances that inhibit or support influenza virus replication. We describe here the enhancing effect of the polyene antibiotic amphotericin B (AmB) on influenza virus growth in Vero cells. We show that isolation rates of influenza A and B viruses from clinical samples can be dramatically enhanced by adding AmB to the culture medium. We demonstrate that AmB promotes the viral uptake and endocytic processing of the virus particles. This effect is specific for Vero and human nasal epithelial cells and was not observed in Madin-Darby canine kidney cells. The effect of AmB was subtype specific and more prominent for human seasonal influenza strains but absent for H5N1 human viruses. The AmB-enhancing effect seemed to be solely due to the viral hemagglutinin function. Our results indicate that the use of AmB may facilitate influenza virus isolation and production in Vero cells.
Genetic Deficiency of Itgb2 or ItgaL Prevents Autoimmune Diabetes Through Distinctly Different Mechanisms in NOD/LtJ Mice (2009)

Together, these data demonstrate that genetic deficiency of Itgb2 or ItgaL confers protection against autoimmune diabetes through distinctly different mechanisms.
Characteristics of dusty plasma instabilities: Pressure and power dependences (2013)

Poster
A review of dry (CO2) reforming of methane over noble metal catalysts (2014)

Dry (CO2) reforming of methane (DRM) is a well-studied reaction that is of both scientific and industrial importance. This reaction produces syngas that can be used to produce a wide range of products, such as higher alkanes and oxygenates by means of Fischer-Tropsch synthesis. DRM is inevitably accompanied by deactivation due to carbon deposition. DRM is also a highly endothermic reaction and requires operating temperatures of 800-1000 °C to attain high equilibrium conversion of CH4 and CO2 to H2 and CO and to minimize the thermodynamic driving force for carbon deposition. The most widely used catalysts for DRM are based on Ni. However, many of these catalysts undergo severe deactivation due to carbon deposition. Noble metals have also been studied and are typically found to be much more resistant to carbon deposition than Ni catalysts, but are generally uneconomical. Noble metals can also be used to promote the Ni catalysts in order to increase their resistance to deactivation. In order to design catalysts that minimize deactivation, it is necessary to understand the elementary steps involved in the activation and conversion of CH4 and CO2. This review will cover DRM literature for catalysts based on Rh, Ru, Pt, and Pd metals. This includes the effect of these noble metals on the kinetics, mechanism and deactivation of these catalysts.
Rock-Mechanical Model of Thin-Bedded Sands and Shales for Improved Fracture Height Prediction, Mesaverde Sandstone, Wyoming (1999)

Abstract The purpose of this study is to construct a core- and log-based rock-mechanical model of thin-bedded sands and shales. The study area is North LaBarge field, Wyoming, where rocks of the Cretaceous Mesaverde Formation produce hydrocarbons from low-permeability, thin-bedded (0.5-10 in; 1-25 cm) sands and shales. Hydraulic fracturing is necessary to obtain production from this reservoir. For roughly 90 ft (27 m) of core, permeabilities and p- and s-wave velocities were measured every 0.5 in (1.2 cm) using probe-type devices. Laboratory results were converted to subsurface conditions using pressurized core plugs, a full-waveform sonic log, and a density log. The resulting rock-mechanical model provides a detailed stress profile which can be used as input into computer models which predict fracture height. Such models generally use bulk-rock properties in which fracture heights are overestimated because the models do not take into account the ability of thin shales to impede fracture propagation.
Challenges for the evaluation ofStaphylococcus aureusprotein based vaccines: Monitoring antigenic diversity (2011)

Clumping factors A (ClfA) and B (ClfB) are Staphylococcus aureus virulence proteins that are displayed on the cell surface of the organism and have potential as vaccine antigens for the prevention of S. aureus disease. Here we evaluate the phylogeny of S. aureus in the context of antigenic variation of these two surface proteins. ClfA and ClfB gene sequences, along with epidemiological markers (MLST, spa and capsule genotype) were obtained for 224 S. aureus isolates including both historical strains and a collection representative of current MRSA isolates from the United States. Variation within ClfA and ClfB was consistent with the established population biology of S. aureus, namely, that S. aureus strains belong to a relatively small number of clonal lineages, with evolution proceeding mainly by mutation and with little to no recombination between clades. Thus most variation in ClfA and ClfB occurs between but not within lineages, and particular groups of ClfA and ClfB variants are closely linked. This has important implications for vaccine development and assessment as it suggests that a relatively small survey of strains will be representative of the total population variation, whereas for species that evolve mainly by recombination, such as Neisseria meningitidis, analysis of a much larger number of strains is needed to accomplish the same purpose. Our study also revealed evidence for the de-evolution of ClfB and therefore its reduced suitability as a target for vaccine development compared to ClfA.
Photonic crystal nanocavity array laser (2005)

We demonstrate a new type of laser composed of an array of coupled photonic crystal nanocavities that enables high differential quantum efficiency and output power, together with a low threshold power comparable to those of single photonic crystal cavity lasers. In our experiment, the laser efficiency increases faster than the lasing threshold with an increase in the number of coupled cavities. We observe a single mode lasing and measure the output powers that are two orders of magnitude higher than in single nanocavity lasers. Finally, we study the laser behavior theoretically and show that the benefits resulting from the coupling of cavities are due to strong cavity effects such as the enhanced spontaneous emission rate.
CD28-B7 T CELL COSTIMULATORY BLOCKADE BY CTLA4Ig IN THE RAT RENAL ALLOGRAFT MODEL1,2 (1996)

Blocking CD28-B7 T-cell costimulation by CTLA4Ig induces tolerance to rat renal allografts and inhibits Th1, but spares Th2, cytokines. We now report on the mechanisms of CD28-B7 blockade in this model. Lymphocytes from CTLA4Ig-treated animals showed significant reduction of mixed lymphocyte response, as well as antidonor cytotoxic T-cell effector function, as compared with rejecting controls. Flow cytometry studies on sera of renal allograft recipients showed complete inhibition of antidonor humoral responses by CTLA4Ig. Analysis by reverse transcriptase-polymerase chain reaction and immunohistology showed that intragraft macrophage products, monocyte chemoattractant protein-1 and inducible nitric oxide synthase, were reduced by CTLA4Ig therapy. Immunohistologic studies also showed reduced intragraft macrophage infiltration and decreased staining for the fibrogenic and mitogenic growth factor, transforming growth factor-beta. These results indicate that CD28-B7 blockade inhibits cell-mediated and humoral immune responses, and suggest that strategies targeting T-cell costimulation may provide a novel approach to prevent chronic allograft rejection.
Stochastic Vector Techniques in Ground-State Electronic Structure (2022)

We review a suite of stochastic vector computational approaches for studying the electronic structure of extended condensed matter systems. These techniques help reduce algorithmic complexity, facilitate efficient parallelization, simplify computational tasks, accelerate calculations, and diminish memory requirements. While their scope is vast, we limit our study to ground-state and finite temperature density functional theory (DFT) and second-order many-body perturbation theory. More advanced topics, such as quasiparticle (charge) and optical (neutral) excitations and higher-order processes, are covered elsewhere. We start by explaining how to use stochastic vectors in computations, characterizing the associated statistical errors. Next, we show how to estimate the electron density in DFT and discuss effective techniques to reduce statistical errors. Finally, we review the use of stochastic vectors for calculating correlation energies within the second-order Møller-Plesset perturbation theory and its finite temperature variational form. Example calculation results are presented and used to demonstrate the efficacy of the methods.
Preoperative electrophysiological characterization of patients with primary macula-involving rhegmatogenous retinal detachment (2018)

Patients with rhegmatogenous retinal detachment have preoperative outer retinal dysfunction equally affecting both rod- and cone-driven pathways, and they have minimal inner retinal dysfunction.
Bifunctional highly fluorescent hollow porous microspheres made of BaMoO4 : Pr3+ nanocrystals via a template-free synthesis (2011)

We report a bifunctional hollow porous microsphere composed of single-component BaMoO4 : Pr3+ nanocrystals by a facile template-free synthesis. All the as-synthesized hollow microspheres are well-dispersed with a diameter of 2–4 μm and the BaMoO4 : Pr3+ nanocrystals measure 30–60 nm in diameter. It is observed that there are a large amount of pores with an average diameter is 17.5 nm in the shell of these BaMoO4 : Pr3+ hollow microspheres, thereby exhibiting a great promise for drug delivery. Meanwhile, the strong, narrow-bandwidth red emission centered at 643 nm from these nanostructures can be efficiently excited from 430 nm to 500 nm. The combination of excellent luminescent properties and a hollow porous nanostructure suggest a great promise in the application of these nanostructures in lighting and displays, and in biomedicine such as targeted drug delivery, integrated imaging, diagnosis, and therapeutics. In addition, the template-free solution synthesis can be applied to the design and fabrication of other functional architectures.
MRI-Compatible Pneumatic Robot for Transperineal Prostate Needle Placement (2008)

Magnetic resonance imaging (MRI) can provide high-quality 3-D visualization of prostate and surrounding tissue, thus granting potential to be a superior medical imaging modality for guiding and monitoring prostatic interventions. However, the benefits cannot be readily harnessed for interventional procedures due to difficulties that surround the use of high-field (1.5T or greater) MRI. The inability to use conventional mechatronics and the confined physical space makes it extremely challenging to access the patient. We have designed a robotic assistant system that overcomes these difficulties and promises safe and reliable intraprostatic needle placement inside closed high-field MRI scanners. MRI compatibility of the robot has been evaluated under 3T MRI using standard prostate imaging sequences and average SNR loss is limited to 5%. Needle alignment accuracy of the robot under servo pneumatic control is better than 0.94 mm rms per axis. The complete system workflow has been evaluated in phantom studies with accurate visualization and targeting of five out of five 1 cm targets. The paper explains the robot mechanism and controller design, the system integration, and presents results of preliminary evaluation of the system.
Centrosome Reduction During Gametogenesis and Its Significance1 (2004)

Animal spermatids and primary oocytes initially have typical centrosomes comprising pairs of centrioles and pericentriolar fibrous centrosomal proteins. These somatic cell-like centrosomes are partially or completely degenerated during gametogenesis. Centrosome reduction during spermiogenesis comprises attenuation of microtubule nucleation function, loss of pericentriolar material, and centriole degeneration. Centrosome reduction during oogenesis is due to complete degeneration of centrioles, which leads to dispersal of the pericentriolar centrosomal proteins, loss of replicating capacity of the spindle poles, and switching to acentrosomal mode of spindle organization. Oocyte centrosome reduction plays an important role in preventing parthenogenetic embryogenesis and balancing centrosome number in the embryonic cells.
OUTCOMES OF PARTIAL NEPHRECTOMY AFTER PREVIOUS RADIOFREQUENCY ABLATION: THE NCI EXPERIENCE (2008)

You have accessJournal of Urology1 Apr 2008OUTCOMES OF PARTIAL NEPHRECTOMY AFTER PREVIOUS RADIOFREQUENCY ABLATION: THE NCI EXPERIENCE Keith J Kowalczyk, H Brooks Hooper, W Marston Linehan, Peter A Pinto, Bradford J Wood, and Gennady Bratslavsky Keith J KowalczykKeith J Kowalczyk More articles by this author , H Brooks HooperH Brooks Hooper More articles by this author , W Marston LinehanW Marston Linehan More articles by this author , Peter A PintoPeter A Pinto More articles by this author , Bradford J WoodBradford J Wood More articles by this author , and Gennady BratslavskyGennady Bratslavsky More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(08)60617-5AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "OUTCOMES OF PARTIAL NEPHRECTOMY AFTER PREVIOUS RADIOFREQUENCY ABLATION: THE NCI EXPERIENCE." The Journal of Urology, 179(4S), p. 214 © 2008 by American Urological AssociationFiguresReferencesRelatedDetailsCited byCampbell S, Novick A, Belldegrun A, Blute M, Chow G, Derweesh I, Faraday M, Kaouk J, Leveillee R, Matin S, Russo P and Uzzo R (2018) Guideline for Management of the Clinical T1 Renal MassJournal of Urology, VOL. 182, NO. 4, (1271-1279), Online publication date: 1-Oct-2009. Volume 179Issue 4SApril 2008Page: 214 Advertisement Copyright & Permissions© 2008 by American Urological AssociationMetricsAuthor Information Keith J Kowalczyk More articles by this author H Brooks Hooper More articles by this author W Marston Linehan More articles by this author Peter A Pinto More articles by this author Bradford J Wood More articles by this author Gennady Bratslavsky More articles by this author Expand All Advertisement PDF downloadLoading ...
<title>Interactive graphics system for locating plunge electrodes in cardiac MRI images</title> (1991)

Understanding the mechanisms of ventricular fibrillation and defibrillation requires analysis of epicardial and endocardial potentials throughout the heart. Plunge electrodes permit recording of cardiac potentials at epicardial and endocardial sites, and allow determination of electrical gradients. They also enable us to determine the arrhythmia recurrence sites following failed defibrillation; these sites may be epicardial or endocardial. Therefore, it is necessary to relate the position of the plunge electrodes to the cardiac geometry. We have developed an interactive, computer graphics based system that allows us to locate plunge electrodes on digitized MRI slices of a heart. The system, which can work with any type of image, allows us to identify the epicardial and endocardial points of each plunge electrode on the different MRI slices. Up to 128 different plunge electrodes may be identified to the system. Normalized 3-D coordinates for each epicardial and endocardial electrode point are computed and stored in data files on the computer. Geometry information obtained from this system permits a more thorough understanding of the electrical signals recorded by the plunge electrodes. This information can be used in the study of cardiac excitation and arrhythmias and could help in the development of a more effective lead system for ventricular defibrillation.
A Genetic Screen Identifies Hypothalamic Fgf15 as a Regulator of Glucagon Secretion (2016)

The counterregulatory response to hypoglycemia, which restores normal blood glucose levels to ensure sufficient provision of glucose to the brain, is critical for survival. To discover underlying brain regulatory systems, we performed a genetic screen in recombinant inbred mice for quantitative trait loci (QTL) controlling glucagon secretion in response to neuroglucopenia. We identified a QTL on the distal part of chromosome 7 and combined this genetic information with transcriptomic analysis of hypothalami. This revealed Fgf15 as the strongest candidate to control the glucagon response. Fgf15 was expressed by neurons of the dorsomedial hypothalamus and the perifornical area. Intracerebroventricular injection of FGF19, the human ortholog of Fgf15, reduced activation by neuroglucopenia of dorsal vagal complex neurons, of the parasympathetic nerve, and lowered glucagon secretion. In contrast, silencing Fgf15 in the dorsomedial hypothalamus increased neuroglucopenia-induced glucagon secretion. These data identify hypothalamic Fgf15 as a regulator of glucagon secretion.
Mild, Selective, and High‐Yield Oxidation of Hantzsch 1,4‐Dihydropyridines with Lead(IV) Acetate. (2005)

Abstract For Abstract see ChemInform Abstract in Full Text.
XIAP variants in male Crohn's disease (2014)

This study reveals the unanticipated, frequent occurrence of XIAP variants in male paediatric-onset CD. The link between XIAP and NOD1/2, and the association of XIAP variants with XLP2, support the concept of PID in a subset of IBD patients. Moreover, these studies provide a rationale for the implementation of XIAP sequencing in clinical diagnostics in male patients with severe CD.