gebhart/patrepeval huggingface dataset:
Reduced BMP Signaling Results in Hindlimb Fusion with Lethal Pelvic/Urogenital Organ Aplasia: A New Mouse Model of Sirenomelia (2012)

Sirenomelia, also known as mermaid syndrome, is a developmental malformation of the caudal body characterized by leg fusion and associated anomalies of pelvic/urogenital organs including bladder, kidney, rectum and external genitalia. Most affected infants are stillborn, and the few born alive rarely survive beyond the neonatal period. Despite the many clinical studies of sirenomelia in humans, little is known about the pathogenic developmental mechanisms that cause the complex array of phenotypes observed. Here, we provide new evidences that reduced BMP (Bone Morphogenetic Protein) signaling disrupts caudal body formation in mice and phenocopies sirenomelia. Bmp4 is strongly expressed in the developing caudal body structures including the peri-cloacal region and hindlimb field. In order to address the function of Bmp4 in caudal body formation, we utilized a conditional Bmp4 mouse allele (Bmp4(flox/flox)) and the Isl1 (Islet1)-Cre mouse line. Isl1-Cre is expressed in the peri-cloacal region and the developing hindimb field. Isl1Cre;Bmp4(flox/flox) conditional mutant mice displayed sirenomelia phenotypes including hindlimb fusion and pelvic/urogenital organ dysgenesis. Genetic lineage analyses indicate that Isl1-expressing cells contribute to both the aPCM (anterior Peri-Cloacal Mesenchyme) and the hindlimb bud. We show Bmp4 is essential for the aPCM formation independently with Shh signaling. Furthermore, we show Bmp4 is a major BMP ligand for caudal body formation as shown by compound genetic analyses of Bmp4 and Bmp7. Taken together, this study reveals coordinated development of caudal body structures including pelvic/urogenital organs and hindlimb orchestrated by BMP signaling in Isl1-expressing cells. Our study offers new insights into the pathogenesis of sirenomelia.
Fundamental optical properties of InN grown by epitaxial lateral overgrowth method (2013)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation Tatsuma Kametani, Jumpei Kamimura, Yuta Inose, Hideyuki Kunugita, Akihiko Kikuchi, Katsumi Kishino, Kazuhiro Ema; Fundamental optical properties of InN grown by epitaxial lateral overgrowth method. AIP Conf. Proc. 4 December 2013; 1566 (1): 107–108. https://doi.org/10.1063/1.4848308 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAIP Publishing PortfolioAIP Conference Proceedings Search Advanced Search |Citation Search
Endogenous adenosine modulates epileptiform activity in rat hippocampus in a receptor subtype‐dependent manner (2004)

The purine nucleoside adenosine is released during seizure activity and exerts an anticonvulsant influence through inhibition of glutamate release and hyperpolarization of neurons via adenosine A(1) receptors. However, activation of adenosine A(2A) and A(3) receptors may counteract the inhibitory effects of A(1) receptors. We have therefore examined the extent to which endogenous adenosine released during seizure activity activates the different adenosine receptor subtypes and the implications for seizure activity in the rat hippocampus in vitro. Brief trains of high-frequency stimulation in nominally Mg(2+)-free artificial cerebrospinal fluid evoked epileptiform activity and resulted in a transient depression of the simultaneously recorded CA1 field excitatory postsynaptic potential. In the presence of 8-cyclopentyl-1,3-dimethylxanthine (CPT), an adenosine A(1) receptor antagonist, the occurrence of spontaneous seizure activity was greatly increased as was the duration and intensity of evoked seizures, whilst the postictal depression of basal synaptic transmission was greatly attenuated. Application of ZM 241385, an adenosine A(2A) receptor antagonist, shortened the duration of epileptiform activity, whereas administration of MRS 1191, an adenosine A(3) receptor antagonist, both decreased the duration and intensity of seizures. Combined application of the A(2A) and A(3) receptor antagonists also resulted in a reduction in seizure duration and intensity. However, no evidence was found for a role for protein kinase C in the regulation of seizure activity by endogenous adenosine. Our data confirm the dominant anticonvulsant role that endogenous and tonic adenosine play via the A(1) receptor, and suggest that the additional adenosine receptor subtypes may compromise this anticonvulsant property through promotion of seizure activity.
Block length distribution, morphology, and property of thermoplastic polyurethanes affected by production method: A polymer‐by‐process investigation (2023)

Abstract Two thermoplastic polyurethanes (TPUs) based on the same monomers and composition were produced by two different production methods – hand mix batch process and continuous band casting. The soft segment was poly(hydrofuran) (PTHF) with a molar mass of 1000 and the hard segment was made of 1,4‐butanediol (BD) and 4,4′‐methylene diphenyl diisocyanate (MDI). The hard segment content amounted to 42%. The distinctions in crystallization and thermal behavior and mechanical properties of the two TPUs were ascribed to differences in the hard and soft block length distribution caused by the different production methods. Using thermal fractionation – a series of successive self‐nucleation and annealing steps – the minor differences in hard block length distribution could be shown and quantified. The length distribution of the hard and soft blocks of the machine‐produced sample was narrower than that of the hand‐cast sample. The former with the narrow block distribution showed thicker and mechanically and thermally more stable hard domains. The more uniform block length distribution facilitated crystallization and resulted in improved tensile recovery behavior and elasticity. The second with the broader distribution, however, showed the highest tensile strength at break, which was ascribed to an improved strain‐induced hardening of the soft phase.
Prevalence of medically induced psychological trauma and its influence on women's health (2023)

We found no relationship between the medical trauma, adverse childhood events, cardiovascular disease, or substance abuse. The presence of medical trauma was associated with the patient's body mass index (calculated as weight in kilograms divided by the square of height in meters).
Reliability Evaluation of Direct Chip Attached Silicon Carbide (2004)

An accelerated stress test (AST) protocol has been developed and used to evaluate the reliability of 6H-silicon carbide (SiC) pressure transducers for operation up to 400 o C for 100 hours. After several cyclic excursions to 400 o C, the maximum drift of the zero pressure offset voltage at 25 o C was 1.9 mV, while the maximum drift at 400 o C was 2.0 mV. The full-scale sensitivity to pressure before and after the AST was 36.6 µV/V/psi at 25 o C and 20.5 µV/V/psi at 400 o C, with a maximum drift of ± 1 µV/V/psi. No systematic degradation of the zero pressure offset was observed.
The catastrophe modeling response to Hurricane Katrina (2008)

Hurricane Katrina was the most destructive US natural disaster in history and the most expensive catastrophe loss ever for the global insurance industry. The occurrence of Hurricane Katrina, and the associated flooding of New Orleans, was also by far the greatest US catastrophe to have occurred since the widespread application of catastrophe models in the mid 1990s. The combination of catastrophic wind and flood losses proved to be a potent test of the underlying methodologies and procedures of catastrophe loss modeling. This chapter reviews the impact of Hurricane Katrina and the response of Risk Management Solutions (RMS) to the event, both in the immediate aftermath and then in galvanizing new research to expand the agenda for catastrophe loss modeling in order to gain a more comprehensive perspective on catastrophe risk costs.
Requirements for the high-level expression of murine interleukin-3 cDNA inEscherichia coli (1988)

Murine interleukin-3 (Mu IL-3) cDNA was previously expressed inEscherichia coli using atac promoter and a constitutive high copy number plasmid vector. We found that significant increases in expression levels could be realized by using thetac promoter for the expression of Mu IL-3 in a plasmid vector possessing a temperature-inducible runaway-replicon. In contrast, use of anlpp promoter under similar conditions did not result in an increase in the Mu IL-3 expression level. Significant differences were observed when the expression levels of IL-3 were monitored in variousE. coli hosts having different genetic backgrounds. A mutant ofE. coli which lacks the protease La was found to increase the level of IL-3 produced. This report describes the effect of a specific protease-deficientE. coli host strain, as well as the effect of different promoters and plasmid replicons on the expression levels and stability of a heterologous gene product.
The Application of Quantitative Analytical Constructs for Chemistry Optimization, Monomer Rehearsal and Reactivity Prediction in Solid Phase Library Synthesis (2004)

The use of quantitative dual linker analytical constructs for the high-throughput generation of solid phase reaction data is described. This methodology can be applied to chemistry optimization, monomer rehearsal and monomer reactivity prediction as a tool to aid the production of solid phase compound libraries.
Sequential Study of the Histopathology and Cellular and Humoral Immune Response During the Development of an Autoimmune Orchitis in Wistar Rats (1989)

Wistar rats immunized with an homologous testicular homogenate (TH) and complete Freund's adjuvant, followed by i.v. injection of Bordetella pertussis, developed an autoimmune orchitis (EAO). Animals were studied at 7, 16, 30, 50, and 80 days (d) after the first immunization. An important lesion of the testis only appeared at 50 d, increasing in severity and incidence (77%) at 80 d. Lesions were characterized by a prevalent aspermatogenesis with tubular atrophy and mild interstitial mononuclear infiltrates. Delayed-type hypersensitivity response (DTH) against TH was detected early at 7 d and, except for 16 d, it increased with time, reaching a maximum at 80 d. A good temporal relationship between DTH and histopathology was found. Circulating antibodies to TH, detected by ELISA, were only present in 64% of the animals with testis lesion, while no deposits of IgG or C3 in the seminiferous tubules were seen. We describe a sequence of immunological events, concomitant with pathological changes of the testis, during the development of a severe EAO in Wistar rats.
Solubilization of Carbon Nanotubes with Ethylene-Vinyl Acetate for Solution-Processed Conductive Films and Charge Extraction Layers in Perovskite Solar Cells (2018)

Carbon nanotube (CNT) solubilization via non-covalent wrapping of conjugated semiconducting polymers is a common technique used to produce stable dispersions for depositing CNTs from solution. Here, we report the use of a non-conjugated insulating polymer, ethylene vinyl acetate (EVA), to disperse multi- and single-walled CNTs (MWCNT and SWCNT) in organic solvents. We demonstrate that despite the insulating nature of the EVA, we can produce semitransparent films with conductivities of up to 34 S/cm. We show, using photoluminescence spectroscopy, that the EVA strongly binds to individual CNTs, thus making them soluble, preventing aggregation, and facilitating the deposition of high-quality films. To prove the good electronic properties of this composite, we have fabricated perovskite solar cells using EVA/SWCNTs and EVA/MWCNTs as selective hole contact, obtaining power conversion efficiencies of up to 17.1%, demonstrating that the insulating polymer does not prevent the charge transfer from the active material to the CNTs.
320 Observation of the fracture behavior by the impact test of A1050-ADC12 functionally graded porous aluminum (2012)

Porous aluminum is a lightweight material with high energy absorption properties. In this study, impact test of A1050-ADC12 functionally graded porous aluminum fabricated by friction stir processing (FSP) route was carried out. It was shown that deformation began from low strength A1050 layer. This result is consistence with result of static compression test of A1050-ADC12 functionally graded porous aluminum.
KLF4-mediated upregulation of the NKG2D ligand MICA in acute myeloid leukemia: a novel therapeutic target identified by enChIP (2023)

The immunoreceptor NKG2D, which is expressed on NK cells and T cell subsets is critically involved in tumor immune surveillance. This applies in particular to acute myeloid leukemia (AML), which evades immune detection by downregulation of NKG2D ligands (NKG2D-L), including MICA. The absence of NKG2D-L on AML cells is moreover associated with leukemia stem cell characteristics. The NKG2D/NKG2D-L system thus qualifies as an interesting and promising therapeutic target.Here we aimed to identify transcription factors susceptible to pharmacological stimulation resulting in the expression of the NKG2D-L MICA in AML cells to restore anti-tumor activity. Using a CRISPR-based engineered ChIP (enChIP) assay for the MICA promoter region and readout by mass spectrometry-based proteomics, we identified the transcription factor krüppel-like factor 4 (KLF4) as associated with the promoter. We demonstrated that the MICA promoter comprises functional binding sites for KLF4 and genetic as well as pharmacological gain- and loss-of-function experiments revealed inducible MICA expression to be mediated by KLF4.Furthermore, induction in AML cells was achieved with the small compound APTO253, a KLF4 activator, which also inhibits MYC expression and causes DNA damage. This induction in turn yielded increased expression and cell surface presentation of MICA, thus rendering AML cells more susceptible to NK cell-mediated killing. These data unravel a novel link between APTO253 and the innate anti-tumor immune response providing a rationale for targeting AML cells via APTO253-dependent KFL4/MICA induction to allow elimination by endogenous or transplanted NK and T cells in vivo. Video Abstract.
Encoding Gaussian Curvature in Glassy and Elastomeric Liquid Crystal Solids (Postprint) (2016)

Abstract : We describe shape transitions of thin, solid nematic sheets with smooth, preprogrammed, in-plane director fields patterned across the surface causing spatially inhomogeneous local deformations. A metric description of the local deformations is used to study the intrinsic geometry of the resulting surfaces upon exposure to stimuli such as light and heat. We highlight specific patterns that encode constant Gaussian curvature of prescribed sign and magnitude. We present the first experimental results for such programmed solids, and they qualitatively support theory for both positive and negative Gaussian curvature morphing from flat sheets on stimulation by light or heat. We review logarithmic spiral patterns that generate cone/anti-cone surfaces, and introduce spiral director fields that encode non-localized positive and negative Gaussian curvature on punctured discs, including spherical caps and spherical spindles. Conditions are derived where these cap-like, photomechanically responsive regions can be anchored in inert substrates by designing solutions that ensure compatibility with the geometric constraints imposed by the surrounding media. This integration of such materials is a precondition for their exploitation in new devices.
ChemInform Abstract: Syntheses of Diaza‐18‐Crown‐6 Ligands Containing Two Units Each of 4‐Hydroxyazobenzene, Benzimidazole, Uracil, Anthraquinone, or Ferrocene Groups. (1999)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Correction: Identification of Potential Pathway Mediation Targets in Toll-like Receptor Signaling (2009)

Dataset S1 contains some errors. The correct dataset is available here:


Click here for additional data file.(53K, zip)
Oxidative coupling of methane in the redox cyclic mode over the Ag–La2O3/SiO2 catalytic system (2010)

Synergism of Ag and La 2 O 3 in the Ag–La 2 O 3 /SiO 2 catalytic system provides substantial efficiency in oxidative coupling of methane carried out in the redox cyclic mode. Selectivity to C 2 products can be raised by preliminary injection of small amount of hydrogen to the catalyst.
Increased conidiation associated with progression along the sterigmatocystin biosynthetic pathway (2004)

The Aspergillus nidulans sterigmatocystin (ST) gene cluster contains both regulatory (aflR) and biosynthetic genes (stc genes) required for ST production. A total of 26 genes are in the cluster, 13 of which have been assigned a known function in the biosynthetic pathway. This complex secondary pathway represents a physiological cost to the fungus. We tested the amount of asexual spore production using a series of isogenic lines of A. nidulans, differing only in a mutation in aflR (resulting in a strain containing no ST intermediates) or a mutation in three stc genes that produced either no ST intermediates (ΔstcJ), an early ST intermediate, norsoloroinic acid (ΔstcE) or a late ST intermediate, versicolorin A (ΔstcU). In two independently replicated experiments we compared the numbers of conidia produced by each of these mutant strains and a wild type ST producer in a neutral (growth media) and a host (corn seed) environment. A stepwise increase in asexual spore production was observed with each progressive step in the ST pathway. Thus, the data suggest that recruitment or loss of these secondary metabolite pathway genes has a selective advantage apart from the physiological activity of the metabolite.
Free Energies of Hydration and Pure Liquid Properties of Hydrocarbons from the OPLS All-Atom Model (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFree Energies of Hydration and Pure Liquid Properties of Hydrocarbons from the OPLS All-Atom ModelGeorge Kaminski, Erin M. Duffy, Tooru Matsui, and William L. JorgensenCite this: J. Phys. Chem. 1994, 98, 49, 13077–13082Publication Date (Print):December 1, 1994Publication History Published online1 May 2002Published inissue 1 December 1994https://pubs.acs.org/doi/10.1021/j100100a043https://doi.org/10.1021/j100100a043research-articleACS PublicationsRequest reuse permissionsArticle Views2122Altmetric-Citations283LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Inclusion relations between power methods and matrix methods of limitation (1977)

A matrix method of limitation is a generalization of both ordinary Toeplitz methods and semicontinuous methods. A power method is a generalization of both Abel’s method and Borel’s exponential method. The main concern of this paper is to find necessary and sufficient conditions for the field of a given power method to be included in the field of a given matrix method. The problem is solved for a wide family of power methods which includes all Abel type methods, the logarithmic method, all Borel type methods and others (also nonregular power methods). Preliminary results, which serve as tools in the solution of the main problem, clarify some aspects of the nature of the field of a power method as an FK space.
Transcriptome-Wide Off-Target Effects of Steric-Blocking Oligonucleotides (2020)

Abstract Steric-blocking oligonucleotides (SBOs) are short, single-stranded nucleic acids designed to modulate gene expression by binding to mRNA and blocking access from cellular machinery such as splicing factors. SBOs have the potential to bind to near-complementary sites in the transcriptome, causing off-target effects. In this study, we used RNA-seq to evaluate the off-target differential splicing events of 81 SBOs and differential expression events of 46 SBOs. Our results suggest that differential splicing events are predominantly hybridization-driven, while differential expression events are more common and driven by other mechanisms. We further evaluated the performance of in silico screens for off-target events, and found an edit distance cutoff of three to result in a sensitivity of 14% and false discovery rate of 99%. A machine learning model incorporating splicing predictions substantially improved the ability to prioritize low edit distance hits, increasing sensitivity from 4% to 26% at a fixed FDR. Despite these large improvements in performance, the approach does not detect the majority of events at a false discovery rate below 99%. Our results suggest that in silico methods are currently of limited use for predicting the off-target effects of SBOs.
Wirelength modeling for homogeneous and heterogeneous FPGA architectural development (2009)

This paper describes an analytical model that relates the architectural parameters of an FPGA to the average prerouting wirelength of an FPGA implementation. Both homogeneous and heterogeneous FPGAs are considered. For homogeneous FPGAs, the model relates the lookup-table size, the cluster size, and the number of inputs per cluster to the expected wirelength. For heterogeneous FPGAs, the number and positioning of the embedded blocks, as well as the number of pins on each embedded block is considered. Two applications of the model to FPGA architectural design are also presented.
Endoscopic carpal-tunnel release in cadavera. An investigation of the results of twelve surgeons with this training model. (1994)

Endoscopic carpal-tunnel releases were performed, with use of the two-portal technique described by Chow, on twenty-four fresh or fresh-frozen wrist specimens from cadavera. Twelve surgeons were taught the technique in the cadaver model by an experienced colleague. Nine surgeons performed one endoscopic carpal-tunnel release; three performed three or more. Incomplete release of the transverse carpal ligament was noted in nine specimens (38 per cent). The percentage of incomplete releases was the same for both the surgeons who performed one endoscopic carpal-tunnel release and those who performed three or more. Complications occurred in four specimens (17 per cent) and included lacerations of an ulnar artery and a median nerve, partial laceration of a flexor tendon, and a fracture of the hook of the hamate. The observed complications and incomplete releases of the transverse carpal ligament in this training model emphasize the risks that may occur when a surgeon is first learning this procedure.
Vaccination against B-cell chronic lymphocytic leukemia with trioma cells: preclinical evaluation. (2003)

Trioma-mediated immunization may be a promising adjuvant treatment of human malignancies of the B-cell lineage, particularly of B-CLL, which has still a very poor prognosis. Our in vitro results pave the way for clinical application.