gebhart/patrepeval huggingface dataset:
Plasminogen deficiency does not prevent sodium retention in a genetic mouse model of experimental nephrotic syndrome (2020)

This study shows that mice lacking urinary plasminogen are not protected from ENaC-mediated sodium retention in experimental NS. This points to an essential role of other urinary serine proteases in the absence of plasminogen.
<title>Near-infrared surface-enhanced Raman spectroscopy of biomedically relevant single molecules on colloidal silver and gold clusters</title> (2000)

Surface-enhanced Raman scattering (SERS) is a phenomenon resulting in strongly increased Raman signals form molecules which have been attached to nanometer sized metallic structures. The technique combines fingerprint capabilities of vibrational spectroscopy and ultra sensitive detection limits. Silver or gold colloidal clusters can provide total enhancement factors of about 14 orders of magnitude for non- resonant Raman scattering at near IR excitation. Since non- resonant near IR photons are used, photodecomposition of the probed molecule is avoided or, at least strongly reduced, and relatively high excitation intensities can be applied. In addition to the Stokes Raman signal, that linearly depends on excitation laser intensity, at excitation intensities higher than approximately 10<SUP>5</SUP>-10<SUP>6</SUP> W/cm<SUP>2</SUP> and 10<SUP>7</SUP> W/cm<SUP>2</SUP>, 'pumped' anti-Stokes Raman scattering and surface enhanced hyper Raman scattering, respectively, can be observed. Both effects can provide a non-linear or two-photon Raman probe where the Raman scattering signal depends quadratically on the excitation laser intensity.
A Natural Polymer-Based Crosslinker System for Conformance Gel Systems (2002)

Abstract This paper describes a material derived from natural sources that can be used to crosslink a variety of polymers over a broad temperature range to produce gels for conformance applications. Delayed crosslinked polymer systems have been used for many years in conformance applications. For the past decade, the most widely used system has been based on chromium (+3) crosslinked polyacrylamide. Organic crosslinkers, such as phenol/formaldehyde and polyethyleneimine (PEI) have also been used with a variety of polymers. However, these systems are being scrutinized by governmental agencies and have been scheduled for phase-out in some countries. Because of these issues, a single, environmentally friendly crosslinker that could be used with a variety of polymers over a broad temperature range was the focus of this study. This paper details the laboratory development of an environmentally friendly, natural polyamine crosslinker system. This crosslinker can be used with a variety of polymers, such as polyacrylamide, AMPS/acrylamide, or alkylacrylate polymers. Gels ranging from stiff and ringing type to "lipping" gels have been obtained. The data illustrate a simple, commercially available system that can be applied to field operations. Potential crosslinking mechanism(s) of the system will be discussed.
A low-power, low data-rate, Ultra-wideband receiver architecture for indoor wireless systems (2008)

In this paper, a novel architecture for a low data-rate, low power consumption Impulse Radio (IR) Ultra-Wideband (UWB) receiver is presented in which the received UWB signal is downconverted to a given intermediate frequency (IF) rather than at baseband. This way, the requirement to implement two separate paths for the in-phase and the quadrature components is removed and the power consumption can be reduced. From the IF, the signal is converted to the digital domain by a set of 24 Redundant Signed Digit (RSD) Analog to Digital Converters (ADCs) working at the pulse repetition rate. The digitized signal is then correlated with a predefined template prior to be fed to the bit detector. The trade-offs for the selection of the IF are highlighted and the BER performance of the proposed receiver is evaluated using simulations in 802.15.3a multipath channels. The expected power consumption for the implementation of the receiver using a CMOS process is also provided.
The Effect of Insulin Concentration on Retroendocytosis in Isolated Rat Adipocytes* (1987)

After internalization by isolated rat adipocytes, insulin can be degraded or released intact from the cell, a process termed retroendocytosis. To determine whether the amount of ligand entering the cell could modulate its ultimate intracellular disposition, adipocytes were incubated with 0.4-25 ng/ml radiolabeled insulin for 20 min at 37 C to reach steady state binding and internalization. After this, surface bound insulin was removed by acid extraction and the cells were reincubated in insulin-free 37 C buffer. The fractional rate of release of internalized cell associated radioactivity was similar at all insulin concentrations. However, insulin enhanced the appearance of trichloroacetic acid (TCA)-precipitable material in a dose-dependent manner reaching a maximum at an insulin concentration of 10 ng/ml. At 0.4 ng/ml insulin, 18 +/- 2% of the released radioactivity was TCA precipitable whereas 36 +/- 3% was precipitable at 25 ng/ml. Sephadex G-50 gel chromatography and reverse phase HPLC analysis of the reincubation medium confirmed TCA-precipitable material was intact insulin. To further investigate the dual pathways of intracellular insulin processing, adipocytes were incubated with 0.4 and 25 ng/ml insulin for 20 min, acid extracted to remove surface receptor insulin, and solubilized. Sephadex G-50 and HPLC analysis revealed that proportionately less insulin intermediates and low molecular weight degradation products are found in cells incubated at the higher insulin concentrations. In conclusion, as adipocytes internalize more insulin, less is converted into insulin intermediates and low molecular weight degradation products and more is diverted to retroendocytosis.
Safety and Pharmacokinetics of the Oral TYK2 Inhibitor PF‐06826647: A Phase I, Randomized, Double‐Blind, Placebo‐Controlled, Dose‐Escalation Study (2020)

Selective inhibition of tyrosine kinase 2 (TYK2) may offer therapeutic promise in inflammatory conditions, with its role in downstream pro-inflammatory cytokine signaling. In this first-in-human study, we evaluated the safety, tolerability, and pharmacokinetics (PK) of a novel TYK2 inhibitor, PF-06826647, in healthy participants. This phase I, randomized, double-blind, placebo-controlled, parallel-group study included two treatment periods (single ascending dose (SAD) and multiple ascending dose (MAD)) in healthy participants and a cohort of healthy Japanese participants receiving 400 mg q.d. or placebo in the MAD period (NCT03210961). Participants were randomly assigned to PF-06826647 or placebo (3:1). Participants received a single oral study drug dose of 3, 10, 30, 100, 200, 400, or 1,600 mg (SAD period), then 30, 100, 400, or 1,200 mg q.d. or 200 mg b.i.d. for 10 days (MAD period). Safety (adverse events (AEs), vital signs, and clinical laboratory parameters), tolerability, and PK were assessed. Overall, 69 participants were randomized to treatment, including six Japanese participants. No deaths, serious AEs, severe AEs, or AEs leading to dose reduction or temporary/permanent discontinuation were observed. All AEs were mild in severity. No clinically relevant laboratory abnormalities or changes in vital signs were detected. PF-06826647 was rapidly absorbed with a median time to maximum plasma concentration of 2 hours in a fasted state, with modest accumulation (< 1.5-fold) after multiple dosing and low urinary recovery. PF-06826647 was well-tolerated, with an acceptable safety profile for doses up to 1,200 mg q.d. for 10 days, supporting further testing in patients.
Return of the Neurotropic Enteroviruses: Co-Opting Cellular Pathways for Infection (2021)

Enteroviruses are among the most common human infectious agents. While infections are often mild, the severe neuropathogenesis associated with recent outbreaks of emerging non-polio enteroviruses, such as EV-A71 and EV-D68, highlights their continuing threat to public health. In recent years, our understanding of how non-polio enteroviruses co-opt cellular pathways has greatly increased, revealing intricate host-virus relationships. In this review, we focus on newly identified mechanisms by which enteroviruses hijack the cellular machinery to promote their replication and spread, and address their potential for the development of host-directed therapeutics. Specifically, we discuss newly identified cellular receptors and their contribution to neurotropism and spread, host factors required for viral entry and replication, and recent insights into lipid acquisition and replication organelle biogenesis. The comprehensive knowledge of common cellular pathways required by enteroviruses could expose vulnerabilities amenable for host-directed therapeutics against a broad spectrum of enteroviruses. Since this will likely include newly arising strains, it will better prepare us for future epidemics. Moreover, identifying host proteins specific to neurovirulent strains may allow us to better understand factors contributing to the neurotropism of these viruses.
Supplemental Table 1 from Routine Plasma-Based Genotyping to Comprehensively Detect Germline, Somatic, and Reversion <i>BRCA</i> Mutations among Patients with Advanced Solid Tumors (2023)

&lt;p&gt;Supplemental Table 1&lt;/p&gt;
Therapeutic ultrasound (1998)

Although diagnostic ultrasound is now a multibillion dollar a year industry, the therapeutic applications of ultrasound were initially thought to have more promise. Indeed, the Fry brothers were performing brain surgery on human patients in the early 1950s, and with considerable success. However, therapeutic ultrasound has still not yet gained its promising potential. Recently, there has been an enormous resurgence of interest in this topic, and several major projects are underway at various facilities around the world. Our own substantial efforts in this area at the University of Washington are reviewed, with brief discussions of topics in lithotripsy, ultrasound-enhanced drug delivery, HIFU surgery, and acoustic hemostasis. [Work supported in part by DARPA and the NIH under Grants DK-43881 and R43HL57739.]
Surface Characterization and Manipulation of SWCNT for Sensor Applications (2006)

Development of practical SWCNT-based chemical sensors is impeded because of difficulties in controlling both orientation/morphology in a MEMs-based sensor platform and the purity of nano-material. Methods to purify CNTs, prior to and following device fabrication are described. More critically, methodology to control the physical morphology of SWCNT on a MEMs platform has been applied for sensor applications.
Parallel switching in connection-oriented networks (2003)

Packet switching in connection-oriented networks that may have multiple parallel links between pairs of switches is considered. An efficient packet scheduling algorithm that guarantees a deterministic quality of service to connections with real time constraints is proposed; this algorithm is a generalization of some recent multiprocessor scheduling algorithms, and offers real time performance guarantees similar to those offered by earlier fair scheduling strategies, such as Weighted Fair Queueing and proportional share schemes.
Phenotypic Characteristics Shared by Preeclamptic Patients and an Animal Model of the Syndrome: Report of a Pilot Study (2006)

There is a group of patients that mirror the characteristics of our animal model. Accordingly, at least one etiology of preeclampsia is related to excessive expansion of the extracellular fluid volume.
Effect of thermal processing practices on the properties of superplastic Al-Li alloys (1993)

The effect of thermal processing on the mechanical properties of superplastically formed structural components fabricated from three aluminum-lithium alloys was evaluated. The starting materials consisted of 8090, 2090, and X2095 (Weldalite(TM) 049), in the form of commercial-grade superplastic sheet. The experimental test matrix was designed to assess the impact on mechanical properties of eliminating solution heat treatment and/or cold water quenching from post-forming thermal processing. The extensive hardness and tensile property data compiled are presented as a function of aging temperature, superplastic strain and temper/quench rate for each alloy. The tensile properties of the materials following superplastic forming in two T5-type tempers are compared with the baseline T6 temper. The implications for simplifying thermal processing without degradation in properties are discussed on the basis of the results.
ELISA measurements for "Multi-omic profiles of human non-alcoholic fatty liver disease tissue highlight heterogeneity in disease phenotype" (2015)

ELISA measurements from plasma samples were carried out using the Ciraplex® platform (Aushon Biosystems, Billerica, MA, US). Commercial assays were purchased and measurements were carried out according to instructions provided by the manufacturer. The following 29 targets were analyzed either as single-plex assays or as multiplex assay: hFGFb; hGROa; hLIF;hIFNg; hIL1b; IL4; IL5; hIL6; hIL10; hIL12p70; hIL13; hTNFa; hI309; hIL8; hIP10; hMCP4; hMIP1a; hMIP1b; hCRP; hLeptin; hPAIactive; hResistin; hIGFBP1; hIGFBP3; hIGFBP2; hMIF; hApoA1; hCRP; hAcrp30.
On the occasion of the 40th anniversary of Acta pharmacologica et toxicologica, May 5th, 1985 (1995)

Abstract: The first issue of Acta pharmacologica et toxicologica was circulated on May 5th, 1945, the very day on which World War II ended in Denmark. The journal is the official publication medium of the Scandinavian Pharmacological Societies. The societies are also represented by associate editors in the editorial board of Pharmacological Reviews. On the happy occasion of the anniversary, we were pleased to receive the following congratulation address from the editor of Pharmacological Reviews, James A. Bain, Ph.D., past president of the International Union of Pharmacology, and Professor of Pharmacology, School of Medicine Emory University, Atlanta, Georgia 30322, U.S.A.
Supplementary Figure 2 from Chemogenomic Profiling Provides Insights into the Limited Activity of Irreversible EGFR Inhibitors in Tumor Cells Expressing the T790M EGFR Resistance Mutation (2023)

Supplementary Figure 2 from Chemogenomic Profiling Provides Insights into the Limited Activity of Irreversible EGFR Inhibitors in Tumor Cells Expressing the T790M EGFR Resistance Mutation
Synthesis of 8-(2-4 dinitrophenyl 2-6 aminohexyl) amino–adenosine 5′ triphosphate: biological properties and potential uses (1982)

We have synthetised 8-(2-4 dinitrophenyl 2-6 aminohexyl) amino-adenosine 5' triphosphate (in short : rATP-DNP), a derivative of ATP which carries a dinitrophenyl group. We show that rATP-DNP is a substrate for calf thymus deoxynucleotidyl terminal transferase (EC 2.7.7.31) and E. coli DNA polymerase I (Kornberg polymerase EC 2.7.7.7.). It can therefore be incorporated into DNA molecules by elongation from 3' ends or by nick translation. The incorporated dinitrophenyl group can be recognized by specific antibodies which can then be detected by anti-antibodies coupled to an enzyme. DNP groups could also be introduced into DNA after enzymatic incorporation of 8-aminohexyl adenosine 5' triphosphate and reaction with 1-fluoro-2-4-dinitrobenzene. Thus, DNA molecules carrying DNP groups can ultimately be revealed by enzymatic coloured reactions. Potential uses of this enzymatic labelling as a substitute to the radioactive detection of nucleic acids, are discussed.
Development and implementation of guidelines in allergic rhinitis – an ARIA‐GA2LEN paper (2010)

The links between asthma and rhinitis are well characterized. The Allergic Rhinitis and its Impact on Asthma (ARIA) guidelines stress the importance of these links and provide guidance for their prevention and treatment. Despite effective treatments being available, too few patients receive appropriate medical care for both diseases. Most patients with rhinitis and asthma consult primary care physicians and therefore these physicians are encouraged to understand and use ARIA guidelines. Patients should also be informed about these guidelines to raise their awareness of optimal care and increase control of the two related diseases. To apply these guidelines, clinicians and patients need to understand how and why the recommendations were made. The goal of the ARIA guidelines is to provide recommendations about the best management options for most patients in most situations. These recommendations should be based on the best available evidence. Making recommendations requires the assessment of the quality of available evidence, deciding on the balance between benefits and downsides, consideration of patients’ values and preferences, and, if applicable, resource implications. Guidelines must be updated as new management options become available or important new evidence emerges. Transparent reporting of guidelines facilitates understanding and acceptance, but implementation strategies need to be improved.
DYNAMIC BAYESIAN CLUSTERING (2013)

Clusters of time series data may change location and memberships over time; in gene expression data, this occurs as groups of genes or samples respond differently to stimuli or experimental conditions at different times. In order to uncover this underlying temporal structure, we consider dynamic clusters with time-dependent parameters which split and merge over time, enabling cluster memberships to change. These interesting time-dependent structures are useful in understanding the development of organisms or complex organs, and could not be identified using traditional clustering methods. In cell cycle data, these time-dependent structure may provide links between genes and stages of the cell cycle, whilst in developmental data sets they may highlight key developmental transitions.
ChemInform Abstract: Synthesis of 5‐Carboxamidopyrrolo(3,2‐b)pyridine. (1993)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Interplay between FGF10 and Notch signalling is required for the self-renewal of pancreatic progenitors (2005)

Recent studies have shown that persistent expression of FGF10 in the developing pancreas of transgenic mice results in enhanced and prolonged proliferation of pancreatic progenitors, pancreatic hyperplasia and impaired pancreatic differentiation. These studies have also suggested that FGF10 prevents the differentiation of pancreatic progenitors by maintaining persistent Notch signalling. Here, we provide experimental evidence sustaining the capacity of FGF10 to induce the proliferation of pancreatic precursors, while preventing their differentiation. Using explant cultures of E10.5 isolated dorsal pancreatic epithelium, we found that FGF10 maintained Notch activation and induced the expansion of pancreatic precursors while blocking their differentiation. In addition, by using a gamma-secretase inhibitor, we were able to down-regulate the expression of Hes1, a target gene of the Notch pathway in explant cultures of pancreatic epithelium treated with FGF10. In such explants, the effect of FGF10 on the proliferation and maintenance of pancreatic progenitors was suppressed. These results demonstrate that activation of the Notch pathway is required as a downstream mediator of FGF10 signalling in pancreatic precursor cells.
Responses of Neutrophils to Myeloid Growth Factors (2007)

Colony-stimulating factors (CSFs) have important effects on mature myeloid cells in addition to their regulatory role in haemopoiesis. Exposure of neutrophils to granulocyte macrophage-CSF (GM-CSF) increases chemotaxis, phagocytosis and cytotoxicity and primes the cells for enhanced oxidative metabolism in response to stimuli, such as formylated oligopeptides derived from bacteria (f-Met-Leu-Phe) and endogenous activated complement components (C5a). GM-CSF induces time-dependent changes in neutrophil f-Met-Leu-Phe receptor number and affinity that correspond to changes in functional activity. The neutrophil IgA Fc receptor is also modulated by GM-CSF such that it develops a high affinity state and transduces a phagocytic signal. The ability to regulate the number and activity of mature myeloid effector cells in vivo establishes unique therapeutic opportunities in the area of infectious disease, cancer treatment, bone marrow transplantation and augmentation of host defence in immunodeficient patients.
Rapid degradation of malachite green by CoFe 2 O 4 –SiC foam under microwave radiation (2018)

This study demonstrated rapid degradation of malachite green (MG) by a microwave (MW)-induced enhanced catalytic process with CoFe<sub>2</sub>O<sub>4</sub>-SiC foam. The catalyst was synthesized from CoFe<sub>2</sub>O<sub>4</sub> particles and SiC foam by the hydrothermal method. X-ray diffraction and scanning electron microscopy techniques were used to confirm that CoFe<sub>2</sub>O<sub>4</sub> particles were settled on the surface of SiC foam. In this experiment, a novel fixed-bed reactor was set up with this catalyst for a continuous flow process in a MW oven. The different parameters that affect the MW-induced degradation rate of MG were explored. The MW irradiation leads to the effective catalytic degradation of MG, achieving 95.01% degradation within 5 min at pH 8.5. At the same time, the good stability and applicability of CoFe<sub>2</sub>O<sub>4</sub>-SiC foam for the degradation process were also discussed, as well as the underlying mechanism. In brief, these findings make the CoFe<sub>2</sub>O<sub>4</sub>-SiC foam an excellent catalyst that could be used in practical rapid degradation of MG.
Murine interleukin-4 production with baculovirus: an easy and rapid method for a small scale production of functional interleukins. (1995)

We described a baculovirus expression system for high level production of secreted murine recombinant IL-4. We have constructed a recombinant baculovirus based on Autographa californica polyhedrosis virus, containing both a synthetic PCR-derived murine IL-4 cDNA under the control of the polyhedrin promoter and the lacZ gene under the control of the P10 promoter to allow an easy detection of recombinant virus. The baculovirus IL-4 was fully functional in biological assay and was present under two glycosylated forms in the supernatants of infected Sf9 cells. We also detected a third unglycosylated intracytoplasmic form resulting from a fusion between the 35 first amino acids of polyhedrin and the murine IL-4. Finally, confocal microscopy showed that this recombinant protein was secreted along a classical pathway like in mammalian cells.
Real time autonomous surveillance of pathogens (2023)

The present invention relates, in part, to systems configured to obtain samples from an organism in an autonomous manner. Such systems can employ a cartridge configured to provide bait to attract an organism, as well as channels to store and/or test samples obtained from the organism.
Design of composite inhibitors targeting glutamate carboxypeptidase II: the importance of effector functionalities (2015)

Atomic coordinates of the present structures together with the experimental structure factor amplitudes were deposited at the RCSB Protein Data Bank under accession codes 4P44 (complex with JRB-4-81), 4P45 (complex with JRB-4-73), 4P4B (complex with CTT54), 4P4D (complex with MP1C), 4P4E (complex with MP1D), 4P4F (complex with NC-2-40), 4P4I (complex with T33) and 4P4J (complex with T33D).