gebhart/patrepeval huggingface dataset:
Hemoglobin-Based Oxygen Carrier Provides Heterogeneous Microvascular Oxygenation in Heart and Gut after Hemorrhage in Pigs (2003)

This study showed that a low volume of DCLHb was effective in resuscitation of the microcirculatory oxygenation of the heart and gut back to control levels. Increasing the volume of DCLHb did not cause an additional increase in heart microPO2, but caused hyperoxic microvascular values in the gut to be attained. It is concluded that microcirculatory monitoring in this way elucidates the regional behavior of oxygen transport to the tissue by HBOCs, whereas systemic variables were ineffective in describing their response.
Treatment of Steroid-Resistant Acute Graft-vs-host Disease by In Vivo Administration of an Anti—T-Cell Ricin A Chain Immunotoxin (1988)

The A chain of the toxin ricin has been conjugated by a disulfide bond to a murine monoclonal antibody that recognizes the CD5 (T,p67) antigen present on 95% of peripheral blood T lymphocytes. This immunotoxin was used to treat a patient with severe grade III-IV, steroid-resistant, acute graft-vs-host disease (GvHD) after an allogeneic, human leukocyte antigen-identical bone marrow transplant for acute myelogenous leukemia. Immunotoxin therapy produced a complete clinical response in the skin and gastrointestinal tract. The patient tolerated a 14-day course without symptoms or signs of toxic effects. After two days of therapy, circulating T cells could not be demonstrated by indirect immunofluorescence. After therapy, acute GvHD did not recur. However, seven months after therapy the patient demonstrated mild signs of chronic GvHD that were easily controlled with low-dose immunosuppressive therapy. These findings indicate that an anti-T-cell ricin A chain immunotoxin can be given safely for treatment of acute GvHD and may be an effective therapy for this significant posttransplant complication.
From Fault Tolerance to Security and Back (2003)

The organizers of the first workshop on the Principles of Dependable Systems discuss fault-tolerance and security. They also give an overview of the recent PoDSy workshop and introduce the Whisper paper that was originally presented at the workshop and has been updated for this month's DSO.
Linear B-cell epitopes of the major core protein of human immunodeficiency virus types 1 and 2 (1990)

Nine murine monoclonal antibodies directed to the major core protein p24 of human immunodeficiency virus type 1 (HIV-1) were obtained and then tested by using an epitope mapping system (Pepscan) covering the whole p24HIV1 protein to characterize antigenic domains. Four different linear epitopes were identified. Monoclonal antibodies recognizing three of these epitopes also reacted to p26HIV2 in Western blotting (immunoblotting). A monoclonal antibody specific for the fourth epitope, located at position 179 to 188 of the gag polyprotein p55HIV1 (human T-cell lymphotropic virus type 3B strain), did not react with HIV type 2 (HIV-2) core proteins. The corresponding sequence is constant in all known HIV-2 and simian immunodeficiency virus (SIV) isolates, including a very divergent SIV strain from African green monkeys (SIVagm/tyo). This observation may be relevant to the phylogeny of primate lentiviruses. Two of the conserved epitopes might be immunogenic during natural infection and could therefore be used for diagnosis and prognosis purposes. These two epitopes are AAEWDRVHP and EIYKRWII, starting at positions 209 and 260 of the polyprotein p55HIV1, respectively.
The Video Window (Overview) (2006)

The Video Window is an interactive device developed by the Interaction Research Studio as part of the Equator project, a six-year Interdisciplinary Research Collaboration, funded by the Engineering and Physical Sciences Research Council (EPSRC). During the project, the piece was loaned to various households for field study.
Mobile‐aided screening system for proliferative diabetic retinopathy (2021)

Abstract NeoVascularization (NV) occurs in the Proliferative Diabetic Retinopathy (PDR) stage, where the development progress of new vessels presents a high risk for severe vision loss and blindness. Therefore, early NV detection is primordial to preserve patient's vision. Several automated methods have been proposed to detect the NV on retinograph‐captured fundus images. However, their employment is constrained by the reduced ophthalmologist‐per‐person ratio and the expensive equipment for image capturing. This paper presents a novel method for NV detection in smartphone‐captured fundus images. The implementation of the method on a smartphone having an optical lens for fundus capturing leads to a Mobiles‐Aided‐Screening system of PDR (MAS‐PDR). The challenge is to ensure accurate and robust detection even with moderate quality of fundus image, on reduced execution time. Within this objective, we identify the major criteria of neovascularized vessels which are tortuosity, width, bifurcation, and density. Our main contribution consists in proposing a sharp feature to reflect each criterion on reduced computational complexity processing. Therefore, the features are provided to a random forest classifier to deduce the PDR stage. A dataset raised from publicly databases is used on a 10‐cross validation process where 98.69% accuracy, 97.73% sensitivity, and 99.12% specificity are achieved. To evaluate the robustness, the same experimentation is repeated after applying motion blur filters to the fundus image dataset, where 98.91% accuracy, 96.75% sensitivity, and 100% specificity are deduced. Moreover, NV screening is performed under 3 s when executed in smartphone devices demonstrating the appropriateness of our method to MAS‐PDR.
Platelet-Derived Growth Factor Is Chemotactic for Human Retinal Pigment Epithelial Cells (1985)

The presence of blood or serum in the vitreous cavity has been associated with the formation of cellular membranes in proliferative vitreoretinopathy and after penetrating ocular trauma. Retinal pigment epithelial (RPE) cells are an important component of these membranes. For RPE cells to effectively spread throughout the vitreous cavity and form contractile membranes, cell migration must occur. Serum has been shown to initiate RPE cell migration. Fibronectin (FN), a glycoprotein found in serum, stimulates RPE cell migration but accounts for only part of the stimulatory effect of serum. We report that another serum component, platelet-derived growth factor (PDGF), also stimulates RPE cell migration. Furthermore, the effect of PDGF and FN are additive and together probably account for a large part of the chemotactic activity found in serum.
Zirconium Materials from Mixed Dicarboxylate Linkers: Enhancing the Stability for Catalytic Applications (2014)

Abstract We report the synthesis of two series of zirconium‐based metal–organic frameworks from solutions composed of a mixed‐ligand system (naphthalene dicarboxylic acid/4‐amino‐2,6‐naphthalenedicarboxylic acid or [1,1′‐biphenyl]‐4,4′‐dicarboxylic acid (BPDC)/2‐amino‐[1,1′‐biphenyl]‐4,4′‐dicarboxylic acid). Materials synthesized with low loadings of functionalized linkers exhibit higher long‐term chemical stability and similarly high thermal stability relative to those with 100 % amino linkers. These materials and their corresponding rhodium derivatives prove excellent catalysts in one‐pot reactions. Moreover, the recycling experiments demonstrate an interesting prospect for the long‐term reusability of these catalysts.
A monoclinic crystal with R32 pseudo-symmetry: a preliminary report of Nodamura virus structure determination (1993)

We have crystallized Nodamura virus, a T = 3 icosahedral virus that can infect both mammalian and insect hosts. Crystals are monoclinic, with two crystallographically independent virus molecules per asymmetric unit. Packing analysis reveals a pseudo-rhombohedral (pseudo-C2 in the monoclinic setting) arrangement of virus particles in the crystal lattice. Crystals differ from the R32 symmetry by rotational and translational deviations. The rhombohedral packing arrangement and its failure to describe the exact virus packing is analyzed in detail. The icosahedral threefold axis is rotated from the body diagonal of the pseudo-rhombohedral cell, breaking the rhombohedral symmetry. The C2 pseudo-symmetry breaks down rotationally and/or translationally.
Buried-interfacial reactivity of palladium-coated Fe2O3/FeTi thin films during vacuum or hydrogen annealing (1990)

FeTi is considered a good material for solid state hydrogen storage; however, it must undergo an initial activation by either a vacuum or hydrogen annealing procedure after preparation or exposure to air. Previous studies indicate that the initial activation in vacuum causes a reduction of surface iron oxides by a solid state reaction with FeTi to produce TiO2. Palladium has also been shown to be an effective barrier for eliminating O2 or H2 impurities in typical H2 charging gases from reaching active FeTi. These impurities cause FeTi decomposition to iron and TiO2. In this study, experiments have been performed to investigate interfacial reactions that occur between Fe2O3 and FeTi layers located below protective palladium overlayers. Vacuum annealing procedures were found to crack the palladium overlayer while forming both iron and titanium oxides below the surface. Hydrogen annealing procedures allowed reduction of the subsurface Fe2O3 by FeTi without evidence of cracking within the palladium overlayer. Both procedures produced Fe-Pd alloys, the latter procedure also forming Fe-Pd hydrides at >or=623 K. Results indicate that the application of palladium overlayers on FeTi may be successfully used provide the initial activation is performed in hydrogen environments.
Parallel Optical Pyramidal Image Processing (1988)

Pyramidal processing is a form of multiresolution image analysis in which a primary image is decomposed into a set of different resolution image copies. Pyramidal processing aims to extract and interpret significant features of an image appearing at different resolutions. Digital pyramidal image processing, because of the large number of convolution type operations, is time-consuming. On the other hand, optical pyramidal processors, because of their ease in performing convolution operation, are preferable in real-time image understanding applications. Two methods of optical pyramidal image generation, a Fourier spectrum filtering based, and a local averaging using 2D lens array, are presented. Preliminary experimental results for optical Gaussian, Laplacian and other fivadtree pyramidal image processing are shown. Experimental results, using commercial lifiuid crystal TVs, for a real-time pyramid image generation are presented.
Testing aspheric lenses: New approaches (2010)

Optical metrology provides a unique approach to measuring surfaces, both technical and optical, over a wide measurement range from macro to nano. We present two new approaches to measuring aspheric lenses with increased flexibility. The first is based on a modified Twyman-Green interferometer where multiple sources for the illumination of the aspheric surface with different angles are adopted to achieve a local compensation of the gradient and consequently a reduction of interference fringes. The second is based on a chromatic Fizeau interferometer with a diffractive element as null-optic for the measurement of extreme ultraviolet aspheres.
Création du Centre interdisciplinaire de recherche en biologie – CIRB (2010)

Pr Alain Prochiantz © P. Imbert, Collège de France La recherche en Biologie au Collège de France est distribuée entre plusieurs sites, en France et à l’étranger, distribution géographique qui varie avec les titulaires des chaires. Mais il y existe aussi une recherche en biologie importante sur le site Marcelin Berthelot, portée dans les années récentes par les chaires de Médecine expérimentale, de Physiologie de la perception et de l’action et de Neuropharmacologie, et dont les titulaires res...
Aldol Condensations on Solid Catalysts: A Cooperative Effect between Weak Acid and Base Sites (2002)

An amorphous aluminophosphate (ALPO) catalyst containing weak acid and base centers can carry out the aldol condensation of heptanal with benzaldehyde at much higher rates and selectivities than conventional solid acid (amorphous or crystalline aluminosilicates) or base catalysts (MgO, hydrotalcites, KF/Al2O3, nitrurated ALPO). With the weak acid-base catalyst, the reaction occurs through a bifunctional acid-base mechanism that involves the activation of benzaldehyde, by protonation-polarization of the carbonyl group on the acid sites, and the attack of the enolate heptanal intermediate generated on the basic sites. With this type of bifunctional acid-base catalyst, compounds with weak basicities are already able to undergo the reaction with a much higher selectivity than those obtained on stronger acid or base catalysts.
Highly Efficient White Polymer Light‐Emitting Diodes Based on Nanometer‐Scale Control of the Electron Injection Layer Morphology through Solvent Processing (2008)

Highly efficient white polymer light-emitting diodes (WPLEDs) are demonstrated using neutral conjugated polymer surfactant PFN-OH as the electron-injection layer through solution processing. The electroluminescence spectra and luminance efficiency are strongly dependent on the morphology of the PFN-OH films. The dramatic improvement of device performance is attributed to the efficient hole-blocking ability due to mixed solvent-induced PFN-OH chain aggregation.
Traffic-aware base station doze in cooperative multicell systems (2013)

In this paper a traffic-aware mechanism, named cooperative base station (BS) doze, is introduced and optimized, aimed at exploring the potential of a high spectrum efficiency (SE) technology, coordinated multi-point (CoMP) transmission, for improving energy efficiency (EE) of downlink cellular networks. The key idea is to allow BS idling by exploiting the delay tolerance of some users, and to increase the opportunity of the idling by using CoMP transmission. The cooperative BS doze strategy involves BS time-slot doze pattern, and multicell user scheduling and cooperative pre-coding, which are jointly optimized in a unified framework. Simulation results demonstrate the substantial EE gain of cooperative BS doze over Non-CoMP BS doze.
Phosphorylation of beta-catenin at S33, S37, or T41 can occur in the absence of phosphorylation at T45 in colon cancer cells. (2003)

Several previous studies in a variety of systems have suggested that phosphorylation at S45 of beta-catenin is essential for subsequent phosphorylation at more NH(2)-terminal residues. Using genetic models expressing beta-catenin under endogenous regulation, we find that phosphorylation of beta-catenin at S45 is not required for phosphorylation at residues S33, S37, or T41 in human colon cancer cells, in contrast to prevailing models. These findings suggest that there are important cell- and organism-specific differences in even the most highly conserved signaling pathways and emphasize the importance of examining these pathways in the cancer cell types in which the pathways are actually deregulated.
Enumeration of carbohydrate hydroxyl groups by silylation and matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (2007)

A method for enumerating hydroxyl groups in analytes is described and applied to various carbohydrates. The analytes were derivatized in solution by using trimethylsilylimidazole (TMSI) and the products were analyzed without chromatography by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The mass spectra revealed consecutive product ion peaks that are separated by 72 m/z units. This 72 m/z unit separation is a result of displacement of a hydroxyl hydrogen atom by a trimethylsilyl group. The number of the observed ions was used to confirm the number of hydroxyl groups in each analyte. This method was utilized in the analysis of single and multi-component analytes.
mTORC2 interactome and localization determine aggressiveness of high-grade glioma cells through association with gelsolin (2023)

mTOR complex 2 (mTORC2) has been implicated as a key regulator of glioblastoma cell migration. However, the roles of mTORC2 in the migrational control process have not been entirely elucidated. Here, we elaborate that active mTORC2 is crucial for GBM cell motility. Inhibition of mTORC2 impaired cell movement and negatively affected microfilament and microtubule functions. We also aimed to characterize important players involved in the regulation of cell migration and other mTORC2-mediated cellular processes in GBM cells. Therefore, we quantitatively characterized the alteration of the mTORC2 interactome under selective conditions using affinity purification-mass spectrometry in glioblastoma. We demonstrated that changes in cell migration ability specifically altered mTORC2-associated proteins. GSN was identified as one of the most dynamic proteins. The mTORC2-GSN linkage was mostly highlighted in high-grade glioma cells, connecting functional mTORC2 to multiple proteins responsible for directional cell movement in GBM. Loss of GSN disconnected mTORC2 from numerous cytoskeletal proteins and affected the membrane localization of mTORC2. In addition, we reported 86 stable mTORC2-interacting proteins involved in diverse molecular functions, predominantly cytoskeletal remodeling, in GBM. Our findings might help expand future opportunities for predicting the highly migratory phenotype of brain cancers in clinical investigations.
Experimental investigation of a thermionic converter with Molybdenum electrodes for low temperature applications (2001)

Experiments performed, which investigated the suitability of Molybdenum electrodes, planar converter with 0.5-mm inter-electrode gap for low temperature, energy conversion applications. Results confirmed that for TE<1773 K, the performance of the present converter is superior to that with a tungsten emitter, when operated at near same conditions. The largest difference occurred at TE∼1600 K and Tc∼1000 K, at which the maximum electric power density (3.0 Wc/cm2) is 70% higher than that of the converter with a W emitter, at more than double the output voltage (0.43 vs. 0.21 V) and at lower cesium pressure (∼105 Pa vs. 245 Pa). Differences in the maximum electric power density of 25% and 50% occurred at TE=1700 K, Tc=1000 K and TE=1600 K and Tc=800 K, respectively, and consistently at lower cesium pressure and higher output voltage, which are major advantages for practical applications. The measured electric power density of the present converter of ∼3.22 Wc/cm2 occurred at TE=1673 K, TC=973 K, at slightly higher output voltage (0.49 V), and PC (241 Pa), and at an estimated conversion efficiency of 12.74%.
<title>Peptide sequencing using MALDI on time-of-flight and ion trap mass spectrometers</title> (1996)

Two instruments have been developed in our laboratory for the direct amino-acid sequencing of peptides. The first is a time-of-flight (TOF) mass spectrometer that utilizes a coaxial, curved-field reflectron which provides simultaneous focusing of product ins formed by post- source decay (PSD) and obviates the need for scanning or stepping the reflectron voltage. The second is a matrix-assisted laser desorption/ionization (MALDI) ion trap mass spectrometer (ITMS). In this instrument, MALDI ions are trapped efficiently by ramping the trapping rf field, and a linear mass scale is achieved by modulating the axial excitation voltage during mass scanning. Monoisotopic mass selection and excitation is accomplished using phase- modulated SWIFT (stored waveform inverse Fourier transform) techniques, while efficient CID (collision-induced dissociation) performance is achieved using pulsed introduction of Xe and other heavier gases. Both mass spectrometers have been designed as compact instruments for sequencing peptides in partially fractionated mixtures.
A Novel p -Nitrophenol Degradation Gene Cluster from a Gram-Positive Bacterium, Rhodococcus opacus SAO101 (2004)

p-Nitrophenol (4-NP) is recognized as an environmental contaminant; it is used primarily for manufacturing medicines and pesticides. To date, several 4-NP-degrading bacteria have been isolated; however, the genetic information remains very limited. In this study, a novel 4-NP degradation gene cluster from a gram-positive bacterium, Rhodococcus opacus SAO101, was identified and characterized. The deduced amino acid sequences of npcB, npcA, and npcC showed identity with phenol 2-hydroxylase component B (reductase, PheA2) of Geobacillus thermoglucosidasius A7 (32%), with 2,4,6-trichlorophenol monooxygenase (TcpA) of Ralstonia eutropha JMP134 (44%), and with hydroxyquinol 1,2-dioxygenase (ORF2) of Arthrobacter sp. strain BA-5-17 (76%), respectively. The npcB, npcA, and npcC genes were cloned into pET-17b to construct the respective expression vectors pETnpcB, pETnpcA, and pETnpcC. Conversion of 4-NP was observed when a mixture of crude cell extracts of Escherichia coli containing pETnpcB and pETnpcA was used in the experiment. The mixture converted 4-NP to hydroxyquinol and also converted 4-nitrocatechol (4-NCA) to hydroxyquinol. Furthermore, the crude cell extract of E. coli containing pETnpcC converted hydroxyquinol to maleylacetate. These results suggested that npcB and npcA encode the two-component 4-NP/4-NCA monooxygenase and that npcC encodes hydroxyquinol 1,2-dioxygenase. The npcA and npcC mutant strains, SDA1 and SDC1, completely lost the ability to grow on 4-NP as the sole carbon source. These results clearly indicated that the cloned npc genes play an essential role in 4-NP mineralization in R. opacus SAO101.
An innovative method for assessing autophagy using the FlowSight imaging flow cytometer (P3253) (2013)

Abstract Autophagy is a process in which normal cellular components that accumulate during growth and differentiation are degraded via the lysosome; it is a survival mechanism that reallocates nutrients from unnecessary processes to more vital processes in the cell. Basal levels of autophagy are usually low but can be up-regulated by numerous stimuli including starvation, physiological stress, pharmacological agents and infections. In addition, suppression of autophagy has been associated with cancer, neurodegenerative disorders, infectious diseases and inflammation. During autophagy, cytoplasmic LC3 is processed and recruited to the autophagosomal membranes; therefore, cells undergoing autophagy can be identified by visualizing LC3 puncta using immunofluorescence microscopy. While manual microscopy allows visual identification of autophagy on a per-cell basis, an objective and statistically rigorous assessment is difficult to obtain. To overcome these problems, we used the FlowSight imaging cytometry platform collect imagery of large numbers of cells to assess autophagy in an objective, quantitative, and statistically robust manner. In this study, we demonstrate a method for determining the best image-based parameter for assess the LC3 puncta in starved and non-starved U2OS RFP-LC3 human osteosarcoma reporter cells.