gebhart/patrepeval huggingface dataset:
Chromosome walking on the TCL1 locus involved in T-cell neoplasia. (1993)

The TCL1 locus on chromosome 14 band q32.1 is frequently involved in the chromosomal translocations and inversions with the T-cell receptor genes observed in several T-cell tumors, including T-prolymphocytic leukemias, acute and chronic leukemias associated with the immunodeficiency syndrome ataxia-telangiectasia, and adult T-cell leukemia. All breakpoints cloned in this area have been mapped to 14q32.1, an area distant approximately 10,000 kb from the immunoglobulin heavy-chain gene locus on chromosome 14q band 32.3. Except for two cases of inversion, no physical linkage of the cloned breakpoints has been reported, nor has a gene been identified in this region. Taking advantage of chromosome-walking techniques and of the P1 phage, we cloned and characterized 450 kb of the germ-line TCL1 locus, starting from the breakpoints of two independent T-cell leukemias. We show that all molecular rearrangements characterized so far map to these clones, indicating not only that this region is the target of chromosomal rearrangements occurring in this area but also that both inversion and translocations occur within a 300-kb region in the T-cell leukemias. In the attempt to identify a candidate oncogene responsible for the malignant transformation, a CpG island centromeric to the inversions and to the translocations has been identified. Two probes near the CpG island have detected sequences conserved among species, as well as two transcripts in the K562 human erythroleukemia cell line. On the basis of these data, a model of activation of the putative TCL1 oncogene is suggested.
Copeptin-Spiegel bei Früh- und Neugeborenen (2009)

Einleitung; Copeptin und Vasopressin wird in äquimolarer Menge aus dem Präprohormon gebildet. Copeptin ist nach Blutentnahme stabil und daher leichter messbar. Bei Erwachsenen mit schwerer Sepsis oder bei Herzinsuffizienz sind Copeptin-Werte erhöht. Wie sind die Werte bei Früh- und Neugeborenen?
fib Bulletin 57. Shear and punching shear in RC and FRC elements Technical report (2010)

Shear and punching shear in RC and FRC elements (PDF) <i>fib</i> Bulletins No. 57. Shear and punching shear in RC and FRC elements (workshop proceedings). Technical report (268 pages, ISBN 978-2-88394-097-0, October 2010)
Fabrication and plasma treatment of 3D polycaprolactane tissue scaffolds for enhanced cellular function (2008)

This paper reports a solid free-form fabrication (SFF) technology-based precision extrusion deposition (PED) process to manufacture three-dimensional (3D) polycaprolactane (PCL) scaffolds and their surface treatment with a plasma source for enhanced osteoblast cell adhesion and proliferation. The PED process allows us to manufacture tissue engineering scaffolds based on designed geometry with complete interconnectivity and controllable porosity. The as-fabricated PCL scaffolds have a pattern with a 0/90° strut configuration of 300 µm pore size and 250 µm strut width. In order to improve cellular activity on 3D PCL scaffolds, they were surface-treated with an oxygen-based plasma source. The surface hydrophilicity and total surface energy of PCL was increased with plasma treatment. Comparisons of different plasma treatment times, including 30 seconds, and 1, 2, 3, 5 and 7 minutes, were performed to identify the plasma treatment duration suggesting higher cellular adhesion and proliferation. The maximum value of total surface energy and its components (polar and dispersive) was observed in 3-min treated PCL scaffolds. In addition, the positive effect of plasma treatment was observed in stregth of cell adhesion, which was increased 55% on 3-min plasma-treated scaffolds compared to untreated and other plasma treatment duriations. Cell culture study over a 7-day period also showed that the cell number on 3-min treated scaffolds is 3-fold the number of cells on untreated scaffolds.
Visualisation of statistically periodic coherent structures in turbulent flows using a phase-locked averaging method (2008)

Coherent structures are the focus of many flow investigations, although their contribution to turbulent mixing is not well understood. When occurring together with high intense turbulent mixing, these structures require statistical methods for visualisation and analysis of simulation results obtained using high-resolution numerical schemes, such as LES or DES. The method of 'phase-locked' averaging separates transient processes with a given frequency. This paper reveals an online formulation for the phase-locked averaging technique at a certain frequency to be set by the user. The method is demonstrated for two realistic flow configurations, an isothermal double-concentric combustor flow and a jet-in-crossflow situation.
Optimization of Photovoltaic energy system: A case study of Hanoi city (2012)

Optimize the output power from Photovoltaic system (PV system) is an important issue in using efficiency renewable energy sources. The purpose of this paper is to investigate a method to optimize the output power from the system. And the method is applied to calculate energy produced in Hanoi city in year 2009. In order to calculate the output power and the annual energy from Photovoltaic array, an hybrid system is built and simulated in MATLAB/Simulink. The use of equivalent switching boost converter is performed with the objective of calculating the power generated by the PV system and estimating the losses in the components of the converter during a given time. Also, there is a comparison between the using of boost converter circuit with Pulse Width Modulator (PWM) generator which controls the state of the MOSFET in the converter and its equivalent. The comparison proved that it could be applied to compute losses for a long period with the equivalent circuit.
Kampo Medicine: Evaluation of the Pharmacological Activity of 121 Herbal Drugs on GABAA and 5-HT3A Receptors (2016)

Kampo medicine is a form of Japanese phytotherapy originating from traditional Chinese medicine (TCM). During the last several decades, much attention has been paid to the pharmacological effects of these medical plants and their constituents. However, in many cases, a systematic screening of Kampo remedies to determine pharmacologically relevant targets is still lacking. In this study, a broad screening of Kampo remedies was performed to look for pharmacologically relevant 5-HT3A and GABAA receptor ligands. Several of the Kampo remedies are currently used for symptoms such as nausea, emesis, gastrointestinal motility disorders, anxiety, restlessness, or insomnia. Therefore, the pharmacological effects of 121 herbal drugs from Kampo medicine were analyzed as ethanol tinctures on heterologously expressed 5-HT3A and GABAA receptors, due to the involvement of these receptors in such pathophysiological processes. The tinctures of Lindera aggregata (radix) and Leonurus japonicus (herba) were the most effective inhibitory compounds on the 5-HT3A receptor. Further investigation of known ingredients in these compounds led to the identification of leonurine from Leonurus as a new natural 5-HT3A receptor antagonist. Several potentiating herbs (e.g., Magnolia officinalis (cortex), Syzygium aromaticum (flos), and Panax ginseng (radix)) were also identified for the GABAA receptor, which are all traditionally used for their sedative or anxiolytic effects. A variety of tinctures with antagonistic effects Salvia miltiorrhiza (radix) were also detected. Therefore, this study reveals new insights into the pharmacological action of a broad spectrum of herbal drugs from Kampo, allowing for a better understanding of their physiological effects and clinical applications.
Metamaterials for high power reflectarray design (2016)

Most often, transmission and reflection properties and geometrical constraints (such as size) are the sole considerations in metamaterials design. Typically, power handling is of little concern as most applications are very low power. Here, we consider not only the complex transmission and reflection properties but also the magnitude of the electric fields in and around the structure when designing metamaterials for robustness in high power reflectarray applications.
Deficiency of IL-5 Receptor α-Chain Selectively Influences the Development of the Common Mucosal Immune System Independent IgA-Producing B-1 Cell in Mucosa-Associated Tissues (1999)

Abstract Deletion of IL-5Rα-chain (IL-5Rα−/−) selectively influenced the mucosal IgA responses in vivo. While levels of IgA in mucosal secretions were more reduced in IL-5Rα−/− mice than in wild-type mice, the levels of IgA in serum were not changed. The frequency of IgA-producing cells was reduced in mucosal effector sites (e.g., intestinal lamina propria and nasal passage), but not in inductive sites such as Payer’s patches and nasal-associated lymphoreticular tissues in IL-5Rα−/− mice. IgA-committed (surface IgA+; sIgA+) B-1 cells mainly resided in mucosal effector tissues, while conventional sIgA+ B (B-2) cells formed in mucosal inductive sites of wild-type mice. In contrast, in the effector tissue of IL-5Rα−/− mice, sIgA+ B-1 cells, but not sIgA+ B-2 cells in the inductive site, were significantly reduced. IL-5Rα was more expressed on sIgA+ B-1 cells than was IL-6R, while both IL-5Rα and IL-6R were expressed on sIgA+ B-2 cells in wild-type mice. sIgA+ B-1 cells produced high levels of IgA with rIL-5 rather than of rIL-6 in vitro. Taken together, the findings suggest that the IL-5/IL-5R signaling pathway is critically important for the development of common mucosal immune system independent sIgA+ B-1 cell in mucosal effector tissues in vivo.
Identification of Prosthetic Joint Infection Pathogens Using a Shotgun Metagenomics Approach (2018)

Metagenomic shotgun sequencing is a powerful tool to identify a wide range of PJI pathogens, including difficult-to-detect pathogens in culture-negative infections.
On non-existence of optimal local synchronous bandwidth allocation schemes for the timed-token MAC protocol (2002)

Numerous methods have been proposed to integrate real-time and non-real-time services of the timed-token medium access control (MAC) protocol. One of the key issues in tailoring the timed-token protocol for real-time applications is the synchronous bandwidth allocation (SBA) problem whose objective is to meet both the protocol and deadline constraints. Several non-optimal local SBA schemes and two optimal global schemes have been proposed. Local SBA schemes use only information available locally to each node, and are thus preferred to global schemes because of their lower network-management overhead. However, we formally prove, using the technique of adversary argument, that there does not exist any optimal local SBA scheme. During the preparation for this proof, we also derive a timing property that generalizes the previous findings about the cycle-time properties of the timed-token protocol.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
2,6-Sialic Acid on Platelet Endothelial Cell Adhesion Molecule (PECAM) Regulates Its Homophilic Interactions and (2010)

Antiangiogenesis therapies are now part of the standard rep-ertoireofcancertherapies,butthemechanismsfortheprolifer-ation and survival of endothelial cells are not fully understood.Althoughendothelialcellsarecoveredwithaglycocalyx,littleisknown about how endothelial glycosylation regulates endothe-lial functions. Here, we show that (PECAM). For integrin activation, a mechanosensory complex2,6-sialic acid is necessaryfor the cell-surface residency of platelet endothelial cell adhe-sion molecule (PECAM), a member of the immunoglobulinsuperfamilythatplaysmultiplerolesincelladhesion,mechani-calstresssensing,antiapoptosis,andangiogenesis.Asapossibleunderlying mechanism, we found that the homophilic interac-tions of PECAM in endothelial cells were dependent on 2,6-sialic acid. We also found that the absence of its cytoplasmic region possesses an immunoreceptor tyrosine-2,6-sialic aciddown-regulated the tyrosine phosphorylation of PECAM andrecruitment of Src homology 2 domain-containing protein-ty-rosine phosphatase 2 and rendered the cells more prone tomitochondrion-dependentapoptosis,asevaluatedusingPECAM-deficient endothelial cells. The present findings open up a newpossibility that modulation of glycosylation could be one of thepromising strategies for regulating angiogenesis.
Glycoprotein Synthesis (2021)

This chapter introduces the recent advances on glycoprotein synthesis.
RT001 in Progressive Supranuclear Palsy—Clinical and In-Vitro Observations (2021)

MSCs derived from patients with PSP have elevated basal levels of LPO, ROS, and mitochondrial dysfunction. These findings are reversed after incubation with RT001. In PSP patients, the progression of disease may be reduced by treatment with RT001.
Fine-Tuning the Degree of Stem Cell Polarization and Alignment on Ordered Arrays of High-Aspect-Ratio Nanopillars (2012)

Nanobiomaterials are introducing new capabilities to coordinate cell selection, growth, morphology, and differentiation. Herein, we report that tuning the geometry of ordered arrays of nanopillars (NP) elicits specialized morphologies in adherent cells. Systematic analysis of the effects of the NP radius, height, and spacing reveals that stem cells assume either flattened, polarized, or stellate morphologies in direct response to interpillar spacing. Notably, on NPs of pitch near a critical spacing (d(crit) ≈ 2 μm for C3H10T1/2 cells), cells exhibit rounding of the cell body, pronounced polarization, and extension of narrow axon-like cell projections aligned with the square lattice of the NP array and extending hundreds of micrometers. Furthermore, increasing the NPs' aspect ratio from 12:1 to 50:1 to produce NPs with a corresponding reduction in the NP bending stiffness of 2 orders of magnitude amplified the cellular response and resulted in a previously unseen degree of cell polarization and alignment. The rapid morphological transformation is reproducible on surfaces that maintain key parameters of the NP geometry and spacing, is influenced by the cell seeding density, and persists for different stem cell lines and primary mesenchymal stem cells. The demonstrated ability to support various morphogenetic trends in stem cells by simply tuning the geometry of the NP substrates provides a stepping-stone for the future design of scaffolds where cellular morphology and alignment are crucial.
ChemInform Abstract: N,O‐Selectivity in the Synthesis of 3‐Me‐cycloSal‐ddAMP (1999)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Detection of codeine and fentanyl in saliva, blood plasma and whole blood in 5-minutes using a SERS flow-separation strip (2019)

A simple-to-use device to measure drugs in saliva, blood plasma, and whole blood for point-of-care analysis and treatment of overdose patients has been investigated. A rudimentary flow strip has been developed to separate opioids from these biofluids for analysis by surface-enhanced Raman spectroscopy (SERS). The strips are based on lateral flow assays, in which the antibodies have been substituted by SERS-active pads for detection. Samples of codeine and fentanyl, artificially added to these biofluids, were measured using the strips by a field-usable Raman spectrometer. We report measurement of these drugs in these biofluids from 0.5 to 5 μg mL-1 in 5 minutes. Calculated limits of detection for the spectra suggest that these drugs could be measured at 5 to 20 ng mL-1 with improvements in the strips' separation capability.
Phase response analyses support a relaxation oscillator model of locomotor rhythm generation in Caenorhabditis elegans (2021)

Neural circuits coordinate with muscles and sensory feedback to generate motor behaviors appropriate to an animal's environment. In <i>C. elegans,</i> the mechanisms by which the motor circuit generates undulations and modulates them based on the environment are largely unclear. We quantitatively analyzed <i>C. elegans</i> locomotion during free movement and during transient optogenetic muscle inhibition. Undulatory movements were highly asymmetrical with respect to the duration of bending and unbending during each cycle. Phase response curves induced by brief optogenetic inhibition of head muscles showed gradual increases and rapid decreases as a function of phase at which the perturbation was applied. A relaxation oscillator model based on proprioceptive thresholds that switch the active muscle moment was developed and is shown to quantitatively agree with data from free movement, phase responses, and previous results for gait adaptation to mechanical loadings. Our results suggest a neuromuscular mechanism underlying <i>C. elegans</i> motor pattern generation within a compact circuit.
Predicting Obstructive Sleep Apnea Status With the Reflux Symptom Index in a Sleep Study Population (2020)

2 Laryngoscope, 2020.
A comparison of copper speciation results obtained using electrothermal atomization atomic absorption spectrometry and computer modelled data (1995)

A comparative study is described between the direct determination of copper species obtained by electrothermal atomization atomic absorption spectrometry (ETAAS) and the predicted data using the NSPEC computer speciation program. Inorganic copper nitrate and copper citrate species were determined at different pH levels using ETAAS following a range of solid-phase extraction procedures. The results indicated that the NSPEC speciation program and experimental data identified the anionic species to be dominant. For a relatively simple system the NSPEC program can therefore be used to compare theoretical data with experimental data.
Application of IEC 61850 to outdoor feeder automation (2008)

IEC 61850 is a recently created international standard initially developed for communication networks and systems in substations. However IEC 61850 provides a very flexible solution framework for automation applications outside of the substation as well. This presentation describes how IEC 61850 concepts can be applied for outdoor feeder automation applications.
Speeding-Up the Macroblock Partition Mode Decision in MPEG-2/H.264 Transcoding (2006)

Though H.264 is highly efficient compared to MPEG-2, the wide and deep penetration of MPEG-2 creates a need for co-existence of these technologies and hence creates a need for transcoding. In this paper, we introduce and evaluate a novel macroblock partition mode decision algorithm for inter-frame prediction to be used as part of a high-efficient MPEG-2 to H.264 transcoder. The proposed tools are used to compute an optimal MB coding mode decision with significantly reduced computational complexity. Specifically, we achieve the computational savings by using the following MB information coming from MPEG-2: the MB coding modes in MPEG-2 and the mean and variance of the 16 4×4 sub blocks of the MPEG-2 residual MBs. We use data mining algorithms to develop a decision tree for obtaining the H.264 coding decision modes. Our results show that the proposed algorithm is able to maintain a good picture quality while considerably reducing the number of operations to be performed.
Steady state analysis and performance characteristics of the LLC-type parallel resonant converter (2002)

In this paper, the steady state analysis of a new half-bridge DC-to-DC parallel resonant converter with LLC-type commutation circuit is presented. Based on the state variable transformation approach, a two-dimensional state-plane diagram is constructed to obtain the steady state response. A set of characteristic curves for output conversion and component stresses is derived from the analysis which can be used for the converter design. It is shown that based on these curves, the proposed resonant topology possesses more desirable features than the conventional parallel resonant converter. Also simulation results are presented to verify the theoretical approach.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Impact of Electrostatics on the Chemodynamics of Highly Charged Metal–Polymer Nanoparticle Complexes (2013)

In this work, the impact of electrostatics on the stability constant, the rate of association/dissociation, and the lability of complexes formed between Cd(II), Pb(II), and carboxyl-modified polymer nanoparticles (also known as latex particles) of radius ∼ 50 nm is systematically investigated via electroanalytical measurements over a wide range of pHs and NaNO3 electrolyte concentrations. The corresponding interfacial structure and key electrostatic properties of the particles are independently derived from their electrokinetic response, successfully interpreted using soft particle electrohydrodynamic formalism, and complemented by Förster resonance energy transfer (FRET) analysis. The results underpin the presence of an ∼0.7-1 nm thick permeable and highly charged shell layer at the surface of the polymer nanoparticles. Their electrophoretic mobility further exhibits a minimum versus NaNO3 concentration due to strong polarization of the electric double layer. Integrating these structural and electrostatic particle features with recent theory on chemodynamics of particulate metal complexes yields a remarkable recovery of the measured increase in complex stability with increasing pH and/or decreasing solution salinity. In the case of the strongly binding Pb(II), the discrepancy at pH > 5.5 is unambiguously assigned to the formation of multidendate complexes with carboxylate groups located in the particle shell. With increasing pH and/or decreasing electrolyte concentration, the theory further predicts a kinetically controlled formation of metal complexes and a dramatic loss of their lability (especially for lead) on the time-scale of diffusion toward a macroscopic reactive electrode surface. These theoretical findings are again shown to be in agreement with experimental evidence.
Ultra-low-noise transition edge sensors for the SAFARI L-band on SPICA (2012)

The Far-Infrared Fourier transform spectrometer instrument SAFARI-SPICA which will operate with cooled optics in a low-background space environment requires ultra-sensitive detector arrays with high optical coupling efficiencies over extremely wide bandwidths. In earlier papers we described the design, fabrication and performance of ultra-low-noise Transition Edge Sensors (TESs) operated close to 100mk having dark Noise Equivalent Powers (<i>NEPs</i>) of order 4 × 10<sup>−19</sup>W/&radic;Hz close to the phonon noise limit and an improvement of two orders of magnitude over TESs for ground-based applications. Here we describe the design, fabrication and testing of 388-element arrays of MoAu TESs integrated with far-infrared absorbers and optical coupling structures in a geometry appropriate for the SAFARI L-band (110 − 210 &mu;m). The measured performance shows intrinsic response time &tau; ~ 11ms and saturation powers of order 10 fW, and a dark noise equivalent powers of order 7 × 10<sup>−19</sup>W/&radic;Hz. The 100 × 100&mu;m<sup>2</sup> MoAu TESs have transition temperatures of order 110mK and are coupled to 320×320&mu;m<sup>2</sup> thin-film &beta;-phase Ta absorbers to provide impedance matching to the incoming fields. We describe results of dark tests (i.e without optical power) to determine intrinsic pixel characteristics and their uniformity, and measurements of the optical performance of representative pixels operated with flat back-shorts coupled to pyramidal horn arrays. The measured and modeled optical efficiency is dominated by the 95&Omega; sheet resistance of the Ta absorbers, indicating a clear route to achieve the required performance in these ultra-sensitive detectors.