gebhart/patrepeval huggingface dataset:
Noninvasive 3‐dimensional imaging of liver regeneration in a mouse model of hereditary tyrosinemia type 1 using the sodium iodide symporter gene (2014)

Cell transplantation is a potential treatment for the many liver disorders that are currently only curable by organ transplantation. However, one of the major limitations of hepatocyte (HC) transplantation is an inability to monitor cells longitudinally after injection. We hypothesized that the thyroidal sodium iodide symporter (NIS) gene could be used to visualize transplanted HCs in a rodent model of inherited liver disease: hereditary tyrosinemia type 1. Wild-type C57Bl/6J mouse HCs were transduced ex vivo with a lentiviral vector containing the mouse Slc5a5 (NIS) gene controlled by the thyroxine-binding globulin promoter. NIS-transduced cells could robustly concentrate radiolabeled iodine in vitro, with lentiviral transduction efficiencies greater than 80% achieved in the presence of dexamethasone. Next, NIS-transduced HCs were transplanted into congenic fumarylacetoacetate hydrolase knockout mice, and this resulted in the prevention of liver failure. NIS-transduced HCs were readily imaged in vivo by single-photon emission computed tomography, and this demonstrated for the first time noninvasive 3-dimensional imaging of regenerating tissue in individual animals over time. We also tested the efficacy of primary HC spheroids engrafted in the liver. With the NIS reporter, robust spheroid engraftment and survival could be detected longitudinally after direct parenchymal injection, and this thereby demonstrated a novel strategy for HC transplantation. This work is the first to demonstrate the efficacy of NIS imaging in the field of HC transplantation. We anticipate that NIS labeling will allow noninvasive and longitudinal identification of HCs and stem cells in future studies related to liver regeneration in small and large preclinical animal models.
Continuous Chiral Separation of Amino Acid Derivatives by Enantioselective Liquid−Liquid Extraction in Centrifugal Contactor Separators (2008)

The continuous enantioselective liquid−liquid extraction of aqueous 3,5-dinitrobenzoyl-d,l-leucine (DNB-d,l-leu) by a cinchona alkaloid extractant (CA) in 1,2-dichloroethane using a centrifugal contact separator (CCS) was studied at 294 K. Typical concentrations were in the order of 1 mM for both DNB-d,l-leu and CA. The best results were found at a pH of 6, with 61% yield for DNB-l-leu and an enantiomeric excess of 34%. Back-extraction studies at different pH values showed that the host can be recovered efficiently in a single CCS, provided that pH > 9. Experimental studies indicate that the CCS behaves as an equilibrium extraction stage, even at total throughputs exceeding 50% of the equipment capacity (1.9 L min−1). A previously developed equilibrium stage extraction model was successfully applied to describe the data for both the extraction and the back-extraction experiments.
Do Concerns About More Advanced Pathological Features Increase the Likelihood of Neurovascular Bundle Resection in Black Men Undergoing Radical Prostatectomy? (2004)

At our institutions a similar proportion of black and white men undergo nerve sparing radical prostatectomy, which appears to produce similar clinical outcomes in black and white men.
Using High Performance Computing to Examine the Processes of Neurogenesis Underlying Pattern Separation/Completion of Episodic Information. (2015)

Using High Performance Computing to Examine the Processes of Neurogenesis Underlying Pattern Separation/Completion of Episodic Information - Sandia researchers developed novel methods and metrics for studying the computational function of neurogenesis, thus generating substantial impact to the neuroscience and neural computing communities. This work could benefit applications in machine learning and other analysis activities.
Continuing Threat of Influenza (H5N1) Virus Circulation in Egypt (2011)

Reservoirs for the continuing influenza (H5N1) outbreaks in Egypt are ill-defined. Through active surveillance, we detected highly pathogenic influenza subtype H5 viruses in all poultry sectors; incidence was 5%. No other subtypes were found. Continued circulation of influenza (H5N1) viruses in various regions and poultry sectors perpetuates human exposure in Egypt.
An in vitro functional assay to measure the biological activity of TB vaccine candidate H4-IC31 (2019)

Potency assays for vaccine products are an important regulatory requirement, and are used to assess product quality and consistency prior to lot release for clinical testing. Ideally they measure an established correlate of efficacy or protection. In cases where there is no known correlate of protection, however, a functional assay that measures a biological response to a vaccine can be applied as a potency assay. Here we describe an in vitro assay which quantitatively measures human T cell activation as a biological response to the TB vaccine candidate H4-IC31. The Cytokine Secretion Assay (CSA) is based on the ability of peripheral blood mononuclear cells (PBMCs) from a Bacillus Calmette-Guérin (BCG)-vaccinated human donor to process and respond to H4-IC31. The ability of H4-IC31 to stimulate a cellular immune response is measured through the quantification of secreted IFNγ and is reported as relative stimulatory activity (RSA) compared to an in-house reference standard. The CSA is specific to the H4-IC31 vaccine, determines the RSA of H4-IC31 in the range of 50% to 150% of the reference standard, and is stability indicating as it detects differences in RSA between intact and heat treated H4-IC31. Although the CSA does not provide a link to clinical efficacy, it fulfills the critical requirements for a biological potency test to assess TB vaccine candidates and can be used along with biochemical and immunochemical assays to define a product profile during clinical development, while eliminating the use of animals for product testing.
SoCCom: Automated Synthesis of System-on-Chip Architectures (2022)

We present CAD framework and EDA tool, <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\mathrm{S{\scriptstyle O}CC{\scriptstyle OM}}$ </tex-math></inline-formula> , for automated synthesis of optimized SoC architectures. We delineate a disciplined and streamlined methodology to enable automated IP integration and design optimization. <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\mathrm{S{\scriptstyle O}CC{\scriptstyle OM}}$ </tex-math></inline-formula> supports generation of a wide variety of optimized SoCs by: 1) automating the entire process of intellectual property (IP) standardization and integration; 2) allowing configurable assembly of complex, scalable systems with application-specific subsystems; and 3) enabling optimization and evaluation of generated designs based on area and power constraints. Applications of <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\mathrm{S{\scriptstyle O}CC{\scriptstyle OM}}$ </tex-math></inline-formula> include development of heterogeneous, domain-specific SoCs, rapid register-transfer level (RTL) prototyping of wide-varieties of SoC benchmarks, and many others.
OpcA and PorB are novel bactericidal antigens of the 4CMenB vaccine in mice and humans (2023)

The ability of Neisseria meningitidis Outer Membrane Vesicles (OMV) to induce protective responses in humans is well established and mainly attributed to Porin A (PorA). However, the contribution of additional protein antigens to protection remains to be elucidated. In this study we dissected the immunogenicity of antigens originating from the OMV component of the 4CMenB vaccine in mice and humans. We collected functional data on a panel of strains for which bactericidal responses to 4CMenB in infants was attributable to the OMV component and evaluated the role of 30 OMV-specific protein antigens in cross-coverage. By using tailor-made protein microarrays, the immunosignature of OMV antigens was determined. Three of these proteins, OpcA, NspA, and PorB, triggered mouse antibodies that were bactericidal against several N. meningitidis strains. Finally, by genetic deletion and/or serum depletion studies, we demonstrated the ability of OpcA and PorB to induce functional immune responses in infant sera after vaccination. In conclusion, while confirming the role of PorA in eliciting protective immunity, we identified two OMV antigens playing a key role in protection of infants vaccinated with the 4CMenB vaccine against different N. meningitidis serogroup B strains.
Multiscale Optical Imaging of Complex Fields Based on the Use of Azobenzene Nanomotors (2013)

International audience
Effects of Roll Dynamics in Model Predictive Control for Autonomous Vehicles (2008)

A Model Predictive Control (MPC) approach for autonomous vehicles is presented. We formulate a predictive control problem in order to best follow a given path by controlling the front steering angle. We start from the results presented in [4] and [7], where the MPC problem formulation\nrelies on a simple bicycle model, and reformulate the problem by using a more complex vehicle model including roll dynamics. We present and discuss simulations of a vehicle performing high speed double lane change maneuvers where roll dynamics become relevant. The results demonstrate that the proposed model based design is able to effectively stabilize the vehicle by using a three dimensional vehicle model at the cost of a higher\ncomputational load.
Controlling the Growth of Staphylococcus epidermidis by Layer-By-Layer Encapsulation (2018)

Commensal skin bacteria such as Staphylococcus epidermidis are currently being considered as possible components in skin-care and skin-health products. However, considering the potentially adverse effects of commensal skin bacteria if left free to proliferate, it is crucial to develop methodologies that are capable of maintaining bacteria viability while controlling their proliferation. Here, we encapsulate S. epidermidis in shells of increasing thickness using layer-by-layer assembly, with either a pair of synthetic polyelectrolytes or a pair of oppositely charged polysaccharides. We study the viability of the cells and their delay of growth depending on the composition of the shell, its thickness, the charge of the last deposited layer, and the degree of aggregation of the bacteria which is varied using different coating procedures-among which is a new scalable process that easily leads to large amounts of nonaggregated bacteria. We demonstrate that the growth of bacteria is not controlled by the mechanical properties of the shell but by the bacteriostatic effect of the polyelectrolyte complex, which depends on the shell thickness and charge of its outmost layer, and involves the diffusion of unpaired amine sites through the shell. The lag times of growth are sufficient to prevent proliferation for daily topical applications.
Genome Sequence of the Basidiomycetous Yeast Pseudozyma antarctica T-34, a Producer of the Glycolipid Biosurfactants Mannosylerythritol Lipids (2013)

The basidiomycetous yeast Pseudozyma antarctica T-34 is an excellent producer of mannosylerythritol lipids (MELs), members of the multifunctional extracellular glycolipids, from various feedstocks. Here, the genome sequence of P. antarctica T-34 was determined and annotated. Analysis of the sequence might provide insights into the properties of this yeast that make it superior for use in the production of functional glycolipids, leading to the further development of P. antarctica for industrial applications.
Effect of Tip and Pressure Side Coolant Injection on Heat Transfer Distributions for a Plane and Recessed Tip (2005)

The present study investigates the effects of coolant injection on adiabatic film effectiveness and heat transfer coefficients from a plane and recessed tip of a high pressure turbine first stage rotor blade. Three cases where coolant is injected from (a) five orthogonal holes located along the camber line, (b) seven angled holes located near the blade tip along the pressure side, and (c) combination cases when coolant is injected from both tip and pressure side holes were studied. The pressure ratio (inlet total pressure to exit static pressure for the cascade) across the blade row was 1.2, and the experiments were run in a blow-down test rig with a four-blade linear cascade. The Reynolds number based on cascade exit velocity and axial chord length was 8.61×105 and the inlet and exit Mach numbers were 0.16 and 0.55, respectively. A transient infrared technique was used to measure adiabatic film effectiveness and heat transfer coefficient simultaneously for three blowing ratios of 1.0, 2.0, and 3.0. For all the cases, gap-to-blade span ratio of 1% was used. The depth-to-blade span ratio of 0.0416 was used for the recessed tip. Pressure measurements on the shroud were also taken to characterize the leakage flow and understand the heat transfer distributions. For tip injection, when blowing ratio increases from 1.0 to 2.0, film effectiveness increases for both plane and recessed tip and heat transfer coefficient decreases for both plane and recessed tip. At blowing ratio 3.0, lift-off is observed for both cases. In case of pressure side coolant injection and for plane tip, lift-off is observed at blowing ratio 2.0 and reattachments of jets are observed at blowing ratio 3.0. But, almost no effectiveness is observed for squealer tip at all blowing ratios with pressure side injection with reduced heat transfer coefficient along the pressure side. For combination case, very high effectiveness is observed at blowing ratio 3.0 for both plane and recessed blade tip. It appears that for this high blowing ratio, coolant jets from the tip hit the shroud first and then reattach back onto the blade tip with very high heat transfer coefficients for both plane and recessed blade tip.
The Manufacturing of microencapsulated Microspheres and Novel Approaches for Characterization (1994)

Rechteübertragung 1966-1994
Effect of holding temperature on the development of volatile constituents of white peaches. (1991)

白肉種モモの採取後における品質管理の一助とするため温度条件が香気成分の発現に及ぼす影響を調査した。'白桃'種モモの未熟果 (hard mature) を採取して10°, 20°, 30℃ (85%RH) 下で6日間保存した後香気成分を高真空蒸溜法により回収し, GCおよびGC-MS法により同定, 定量し次の結果を得た。(1) 保存温度と係わりなくモモの香気成分としては微量ピークを含めると約89成分が検出され, そのうち33成分を同定した。(2) 同定した33成分を官能基別にみると, ラクトン類11, アルコール類7, エステル類6, テルペン類4, アルデヒド類2, その他3成分であった。(3) 10℃, 6日保存では青臭みを示すC6アルデヒド・アルコール成分と芳香の主体をなすラクトン類は採取直後と大きな変化はなく香り立ちも不十分であった。(4) 一方, 20。および30℃6日間保存ではほとんどのC6アルデヒド・アルコールの割合は急減し, それに伴いラクトン類は急増し香気が著しく生成した。30℃では青臭みの代表であるt-2-ヘキセノールが著しく増大した。(5) 匂い閾値からみた温度別の香気発現に対する成分の検討から, 20℃と30℃ではC6アルデヒド・アルコールの寄与度が減少し, 芳香を示すラクトン類とリナロールのそれが大きくなることが判明した。(6) 以上よりモモ香気の発現適温は20℃周辺であることが分かった。
Robust Retrieval Augmented Generation for Zero-shot Slot Filling (2021)

Automatically inducing high quality knowledge graphs from a given collection of documents still remains a challenging problem in AI. One way to make headway for this problem is through advancements in a related task known as slot filling. In this task, given an entity query in form of [Entity, Slot, ?], a system is asked to fill the slot by generating or extracting the missing value exploiting evidence extracted from relevant passage(s) in the given document collection. The recent works in the field try to solve this task in an end-to-end fashion using retrieval-based language models. In this paper, we present a novel approach to zero-shot slot filling that extends dense passage retrieval with hard negatives and robust training procedures for retrieval augmented generation models. Our model reports large improvements on both T-REx and zsRE slot filling datasets, improving both passage retrieval and slot value generation, and ranking at the top-1 position in the KILT leaderboard. Moreover, we demonstrate the robustness of our system showing its domain adaptation capability on a new variant of the TACRED dataset for slot filling, through a combination of zero/few-shot learning. We release the source code and pre-trained models.
Ultrafiltrazione peritoneale e sindrome cardiorenale: gestione del sovraccarico di fluidi e ruolo del sodio (2019)

Congestion represents a crucial clinical component of both heart failure and cardiorenal syndrome and it has been postulated to modulate heart and kidney cross-link. Diuretic therapy is a corner stone in the treatment patients with heart failure, and renal replacement therapies are mainly used for patients with refractory heart failure who have not reached the worst stages of renal disfunction. Peritoneal dialysis is a home-based therapeutic modality providing both solute clearance and ultrafiltration, together with relief from congestion in decompensated heart failure patients. The following review will focus on sodium removal in refractory decompensated heart failure patients undergoing peritoneal dialysis. (Cardionephrology)
Calorimetric evidence for allosteric subunit interactions associated with inhibitor binding to band 3 transporter. (1994)

A calorimetric endotherm occurring at 68 degrees C (the C-transition) has been assigned previously to the integral domain of band 3 and was shown to be shifted to 78 degrees C after covalent binding of 4,4'-diisothiocyanostilbene-2,2'-disulfonate (DIDS). In this study, we correlate the fractional appearance of the shifted C-transition with the fraction of DIDS bound to the band 3 monomer population. Our results show a distinctly nonlinear correlation plot with the appearance of the shifted C-transition lagging behind DIDS labeling of the band 3 monomer population. The lag suggests that both monomers of a band 3 dimer must be labeled by DIDS in order for the shifted C-transition to appear at 78 degrees C, implying that the thermal unfolding of the integral domain of band 3 is modulated by allosteric interactions between subunits. This is the first in situ structural evidence supporting ligand-mediated subunit interactions within a "carrier"-type transporter protein oligomer.
MAGNETIC STRUCTURE ANALYSIS OF MULTILAYERED OBLIQUE-EVAPORATED TAPE (1991)

Multilayered oblique-evaporated tapes have been developed which have higher residual magnetization with high coercivity, and can exhibit excellent recording characteristics in comparison with single-layered tapes. A double-layered model is considered as a simple case of multilayered oblique-evaporated tapes. The magnetic structure of this model was calculated in the condition of the minimization of its magnetic energy on the basis of the coherent rotation model including interlayer interactions. Through this analysis, it was found that the multilayered tape of this model can achieve high saturation magnetization with holding high coercivity by the interlayer interaction, even in a state of high column angle. It is understood that the multilayered tape has more excellent recording characteristics over a wide frequency range than single-layered tape through this results.
Synthesis and properties of a corrole with small and electron-withdrawing substitutents, 5,15-bis(trifluoromethyl)-10-pentafluorophenylcorrole (2006)

Several methodologies for the synthesis of corroles that carry minimal-sized electron-withdrawing substituents at the meso- C atoms were investigated; of these the one based on dypyrromethane/aldehyde condensation was fruitful. The new corrole with one C 6 F 5 and two CF 3 groups, as well as its cobalt(III) complex, were fully characterized by spectroscopy and X-ray crystallography.
End-to-End Optimization of Metasurfaces for Imaging with Compressed Sensing (2022)

We present a framework for the end-to-end optimization of metasurface imaging systems that reconstruct targets using compressed sensing, a technique for solving underdetermined imaging problems when the target object exhibits sparsity (i.e. the object can be described by a small number of non-zero values, but the positions of these values are unknown). We nest an iterative, unapproximated compressed sensing reconstruction algorithm into our end-to-end optimization pipeline, resulting in an interpretable, data-efficient method for maximally leveraging metaoptics to exploit object sparsity. We apply our framework to super-resolution imaging and high-resolution depth imaging with a phase-change material. In both situations, our end-to-end framework computationally discovers optimal metasurface structures for compressed sensing recovery, automatically balancing a number of complicated design considerations to select an imaging measurement matrix from a complex, physically constrained manifold with millions ofdimensions. The optimized metasurface imaging systems are robust to noise, significantly improving over random scattering surfaces and approaching the ideal compressed sensing performance of a Gaussian matrix, showing how a physical metasurface system can demonstrably approach the mathematical limits of compressed sensing.
Bioluminescent Assays for Cytochrome P450 Enzymes (2013)

The cytochrome P450 (CYP) family contains 57 enzymes in humans. The activity of CYPs against xenobiotics is a primary consideration in drug optimization efforts. Here we describe a series of bioluminescent assays that enable the rapid profiling of CYP activity against compound collections. The assays employ a coupled-enzyme format where firefly luciferase is used to measure CYP enzyme activity through metabolism of pro-luciferase substrates.
Synthesis, Spectral Characterization, and Antibacterial Activity of Some New Azomethine-Metal Complexes Derived from Vanillin (2013)

Abstract: Synthesis and characterization of Schiff base with solid metal complexes derived from Vanillin and 2-amino-2ethyl-1, 3-propanediol (VAEPD) has been carried out. Structural elucidation of the Schiff base and its metal complexes [Cu(II) and Co(II)] were analyzed on the basis of various spectra like IR, electronic absorption spectra, NMR, ESR, TG-DTA and magnetic moments. From the spectral study, monomeric structures of the complexes showed a six-cordinated metal center moiety with an octahedral geometry. In addition the metal complexes were screened for antibacterial activities and the metal complexes showed moderate activity against the organisms Escherichia coli (E.C), Bacillus subtilis (B.S) and Enterobacter aerogenes.
157-nm Resist Materials Using Main-Chain Fluorinated Polymers (2004)

157-nm lithography is one of the most promising technologies for next-generation lithography. Due to the high absorbance at 157 nm, existing photoresist materials can not be used for 157-nm lithography. Fluoropolymers show good optical transparency and fluorinated resist materials have been intensively studied. Side-chain-fluorinated polymers have been shown a possibility of use for multi-layer resists. However they can not be applied to a single-layer resist, which is the most favored approach among potential industry. We have studied the main-chain-fluorinated polymers that meet the transparency requirement for use as single layer resists. They are two families composed of tetrafluoroethylene and monocyclic fluorocarbons. They showed good absorption coefficients of less than 1.5 μm-1 and fine dry etching durability. Positive-working resists were developed using these fluoropolymers and showed good sensitivity less than 10 mJ/cm2. The resolution of the 80-nm dense pattern was determined at the film thickness thicker than 200 nm.