gebhart/patrepeval huggingface dataset:
Abstract 1473: Dual oncolytic viral immunotherapy generates large polyfunctional tumour-specific CD8 + T cell responses that infiltrate immunosuppressive tumors (2019)

Abstract Generating effective T cell mediated clearance of solid tumors remains an unmet challenge in cancer therapy. Suppressive tumor microenvironments limit the generation of significant numbers of tumor specific T effector cells, their migration to tumor beds, and their subsequent functionality within tumors, thereby blocking the patient’s normally potent acquired immune response from contributing to tumor control. In an effort to meet this challenge, we have developed novel oncolytic viruses that were bioselected and engineered to cause cancer cell death through two distinct and complementary mechanisms-of-action, direct cancer lysis and tumor-antigen specific T cell generation. Specifically, we have selected and engineered MG1 Maraba virus which both infects tumor tissue to reverse immune suppressive programs while simultaneously delivering vector encoded tumor antigens to the spleen to vaccinate against the patient’s tumor. The result is a large increase in peripheral and tumor infiltrating CD8+ T effectors cells, strong intratumoral inflammatory signatures and ultimately curative efficacy in preclinical solid tumor models. This first-in-class therapeutic strategy is currently being evaluated in Phase 1 and Phase 2 clinical trials. In this new study, we describe the development of a novel viral immunotherapy platform based on Farmington virus that is: (1) oncolytic in solid tumor models, (2) a potent inducer of highly-functional antigen-specific T cells (expanding tumor specific CD8+ T effector pools over 1000 fold from pre-existing T central memory) and is (3) immunologically distinct from our clinical MG1 Maraba platform. We show that when used sequentially in a heterologous boosting regimen, T cell responses to encoded tumor antigens, either foreign (HPV E7), self (TRP2) or multi-neoantigen, can exceed greater than 50% of all CD8+ T cells in the periphery. The majority of these CD8+ T effectors show markers of polyfunctionality with little expression of the PD-1 exhaustion marker. We will describe our current data assessing the phenotype, localization and potency of these T cell responses in preclinical models of solid tumors and propose strategies to deploy our novel dual oncolytic viral immunotherapy boosting paradigm to the clinic. Citation Format: David Stojdl. Dual oncolytic viral immunotherapy generates large polyfunctional tumour-specific CD8 + T cell responses that infiltrate immunosuppressive tumors [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 1473.
Development of a Micro-hotplate for different Metal Oxide Gas Sensors with High Operating Temperatures (2007)

International audience
Brain-derived neurotrophic factor can act as a pronecrotic factor through transcriptional and translational activation of NADPH oxidase (2002)

Several lines of evidence suggest that neurotrophins (NTs) potentiate or cause neuronal injury under various pathological conditions. Since NTs enhance survival and differentiation of cultured neurons in serum or defined media containing antioxidants, we set out experiments to delineate the patterns and underlying mechanisms of brain-derived neurotrophic factor (BDNF)-induced neuronal injury in mixed cortical cell cultures containing glia and neurons in serum-free media without antioxidants, where the three major routes of neuronal cell death, oxidative stress, excitotoxicity, and apoptosis, have been extensively studied. Rat cortical cell cultures, after prolonged exposure to NTs, underwent widespread neuronal necrosis. BDNF-induced neuronal necrosis was accompanied by reactive oxygen species (ROS) production and was dependent on the macromolecular synthesis. cDNA microarray analysis revealed that BDNF increased the expression of cytochrome b558, the plasma membrane-spanning subunit of NADPH oxidase. The expression and activation of NADPH oxidase were increased after exposure to BDNF. The selective inhibitors of NADPH oxidase prevented BDNF-induced ROS production and neuronal death without blocking antiapoptosis action of BDNF. The present study suggests that BDNF-induced expression and activation of NADPH oxidase cause oxidative neuronal necrosis and that the neurotrophic effects of NTs can be maximized under blockade of the pronecrotic action.
Can stimulated Raman pumping cause large population transfers in isolated molecules? (2011)

When stimulated Raman pumping (SRP) is applied to a stream of isolated molecules, such as found in a supersonic molecular beam expansion, we show that SRP can neither saturate nor power broaden a molecular transition connecting two metastable levels that is resonant with the energy difference between the pump and Stokes laser pulses. Using the optical Bloch-Feynman equations, we discuss the pumping of the hydrogen molecule from H(2) (v = 0, J = 0, M = 0) to H(2) (v = 1, J = 2, M = 0) as an illustration of how coherent population return severely reduces the SRP pumping efficiency unless the pump and Stokes laser pulses are applied with an appropriate relative delay and ratio of intensities.
DFT Study of Atomically-Modified Alkali-Earth Metal Oxide Films on Tungsten (2014)

The work function and stability of 570 alloyed alkali-earth oxide films on W(100) have been calculated within density functional theory. Computational screening of this large phase space was enabled by utilizing the virtual crystal approximation, where the degree of freedom in the chemical composition is modeled with virtual atoms of mixed Ca, Sr, and Ba character. Low work functions are achieved by doping the films with Sc or Li and alloys containing small amounts of Ca. In particular, Li-doped systems with small Ca content also show favorable stability. Identification of such alloys outperforming any of the constituents relies on careful sampling of the chemical composition. We have identified the interactions within the film that limit the reduction in work function given by the dipole normal to the surface. We have shown that control of the screening of the intrafilm interactions by O atoms is essential for the design of new low work function materials.
Demons algorithms for fluid and curvature registration (2009)

As the number of clinical applications requiring nonrigid image registration continues to grow, it is important to design registration algorithms that not only build on the best available theory, but also are computationally efficient. Thirion's Demons algorithm [1] estimates nonrigid deformations by successively estimating force vectors that drive the deformation toward alignment, and then smoothing the force vectors by convolution with a Gaussian kernel. It essentially approximates a deformation under diffusion regularization [2], and it is a popular choice of algorithm for nonrigid registration because of its linear computational complexity and ease of implementation. In this article, we show how the Demons algorithm can be generalized to handle other common regularizers, yielding O(n) algorithms that employ Gaussian convolution for elastic, fluid, and curvature registration. We compare the speed of the proposed algorithms with algorithms based on Fourier methods [3] for registering serial chest CT studies.
Laser cutting of carbon fiber reinforced thermo-plastics (CFRTP) by single-mode fiber laser irradiation (2014)

We report on the laser cutting of carbon fiber reinforced thermo-plastics (CFRTP) with a cw IR fiber laser (single-mode fiber laser, average power: 350 W). CFRTP is a high strength composite material with a lightweight, and is increasingly being used various applications. A well-defined cutting of CFRTP which were free of debris and thermal-damages around the grooves, were performed by the laser irradiation with a fast beam galvanometer scanning on a multiple-scanpass method.
Sensing of Chiral Fullerenes by a Cyclic Host with an Asymmetrically Distorted π-Electronic Component (2006)

Upon inclusion complexation with a racemic mixture of chiral fullerene C76 ((+/-)-C76), a novel cyclic host (1Rh) composed of a chiral N-methylporphyrin (PNMe) and a methylrhodium porphyrin (PRh) exhibited at 20 degrees C a 1H NMR spectral profile with diastereoisomerically split signals due to the NH group of PNMe. In contrast, because of a faster guest exchange dynamics, metal-free reference 12H, under identical conditions, did not show any signature of such diastereoisomeric splitting. Owing to the excellent resolution (15.6 Hz) of the split NH signals, the complexation with 1Rh allowed for the accurate determination of the optical purity of C76. On the basis of the 1H NMR spectral profiles of 1Rh in toluene-d8 at 20 degrees C in the presence of enantiomer mixtures of C76 of different optical purities, an absolute Deltaepsilon value of 58.5 M-1 cm-1 at 330 nm was obtained for enantiomerically pure C76.
Extensions to RT-Constrain in Hierarchical Route Reflection Scenarios (2017)

The Route Target (RT) Constrain mechanism specified in RFC 4684 is
used to build a route distribution graph in order to restrict the
propagation of Virtual Private Network (VPN) routes. In network
scenarios where hierarchical route reflection (RR) is used, the
existing RT-Constrain mechanism cannot guarantee a correct route
distribution graph. This document describes the problem scenario and
proposes a solution to address the RT-Constrain issue in hierarchical
RR scenarios.
Robust Generation of Oligodendrocyte Progenitors from Human Neural Stem Cells and Engraftment in Experimental Demyelination Models in Mice (2010)

We describe a reliable method to generate large numbers of oligodendrocytes from a renewable source of somatic, non-immortalized NSC from the human foetal brain. We also provide insights on the mechanisms underlying the pro-oligodendrogenic effect of the treatments in vitro and discuss potential issues responsible for the limited myelinating capacity shown by hNSC-derived oligodendrocytes in vivo.
Covalent structural analysis of yeast inorganic pyrophosphatase. (1978)

The present paper describes the amino acid sequence analysis of the internal and COOH-terminal cyanogen bromide fragments of yeast inorganic pyrophosphatase (Sterner, R., Noyes, C., and Heinrikson, R.L. (1974) Biochemistry 13, 91-99). This information coupled with that derived from earlier structural studies of the enzyme (Sterner, R., AND Heinrikson, R.L. (1975) Arch. Biochem. Biophys. 165, 693-703) provides the complete covalent structure of the pyrophosphatase subunit. The majority of the sequence data was derived from automated Edman degradation of the intact cyanogen bromide fragments and the large tryptic peptides obtained from citraconylated derivates in which cleavages were restricted to arginyl residues. The structural determination was completed by analysis of tryptic and chymotryptic peptides from the decitraconylated fragments. The monomer peptide chain contains 285 amino acid residues and the molecular weight calculated from the sequence analysis is 32,042.
Direct 2-Arylation of Thiophene Using Low Loading of a Phosphine-Free Palladium Catalyst (2011)

Abstract The direct coupling of aryl halides with thiophene would be a considerable advantage for sustainable development because of only HBr associated with a base as by-product is formed and the number of steps to prepare these compounds is less than in more classical coupling reactions. We observed that through the use of only 0.2 mol% Pd(OAc)2 as the catalyst, a range of aryl bromides undergoes coupling via a C-H bond activation/functionalization reaction with thiophene to give 2-arylated thiophenes in good yields. In most cases, only traces of polyarylated thiophenes were detected when a large excess of thiophene was employed. This air-stable catalyst can be used with a wide variety of aryl bromides.
Digital Holographic Optics (2009)

This chapter contains sections titled: Conventional Holography Different Types of Holograms Unique Features of Holograms Modeling the Behavior of Volume Holograms HOE Lenses HOE Design Tools Holographic Origination Techniques Holographic Materials for HOEs Other Holographic Techniques
Device parameter optimization for reduced short channel effects in retrograde doping MOSFET's (1996)

A simple technique to optimize the device parameters of the retrograde channel doping MOSFET to minimize short channel effects for a constant threshold voltage is presented in this work. The results indicate that the highest possible substrate doping does not necessarily result in minimum short channel effects.
Expression of Bovine Herpesvirus Type 1 Glycoprotein gI in Transfected Bovine Cells Induces Spontaneous Cell Fusion (1990)

Bovine MDBK cells were transfected with Rous sarcoma virus-based vectors for constitutive expression of the bovine herpesvirus type 1 (BHV-1) glycoprotein, gI. Cell lines stably expressing recombinant gI were cloned and characterized. Recombinant gI was localized intracellularly, predominantly in a perinuclear region, and on the cell surface. Cells expressing gI exhibited spontaneous polykaryon formation, thus confirming the fusogenic activity described previously in gI-expressing transfected murine LMTK- cells. The recombinant form of gI synthesized in transfected MDBK cells was similar in Mr to the form expressed in BHV-1-infected MDBK cells, unlike the recombinant form of gI expressed by LMTK- cells which is deficient in N-linked glycosylation. It was concluded that cell fusion associated with the expression of BHV-1 gI in transfected mammalian cells is a reproducible phenomenon in a number of cell types and is not due to species-specific factors or expression of abnormally glycosylated gI. Cell fusion is a useful in vitro marker for gI function and may contribute to the spread of BHV-1 infections in vivo.
Experiments with acoustic solitons in crystalline solids (2001)

We have used the picosecond ultrasonic technique to study the formation of acoustic solitons. In these experiments, a longitudinal acoustic pulse is generated at one surface of a sample. After the pulse has propagated across the sample, its shape is modified as a result of phonon dispersion and nonlinearity. We have found that the change in the pulse shape can be described by the Korteweg--de Vries equation. The experiments have been performed on Si, MgO, \ensuremath{\alpha}-quartz, and sapphire. For each sample, we have observed fully developed acoustic solitons, with pulse shapes that are in reasonable agreement with the results of computer simulations.
ChemInform Abstract: CHEMICAL AND STRUCTURAL RELATIONSHIPS AMONG THE OLIGOMERIC COMPOUNDS MFE(CO)4 (M = ZINC, CADMIUM, MERCURY), IRON LEAD TETRACARBONYL, COBALT SILVER TETRACARBONYL, AND THEIR LEWIS BASE ADDUCTS (1978)

Abstract Nach bekannten Methoden ‐ ohne Ausb.‐Angaben ‐ werden die Metall‐Metall‐gebundenen Komplexe (I)‐(VII) dargestellt und IR‐spektroskopisch sowie durch Röntgenpulverdiffraktionsuntersuchungen charakterisiert.
Natural antibodies in human sera directed against blood‐group‐related determinants expressed on colon cancer cells (1983)

Sera from 136 normal males and 33 members of families at high risk for colon cancer were tested for reactivity with six colon cancer cell lines by the protein A-mixed hemadsorption assay. Ninety-one sera had antibodies to colon cancer cell line HT-29. In 89 cases the antibodies were absorbed by human A and B erythrocytes or sheep erythrocytes. Antibodies in the remaining two sera, which were from sisters in the high-risk group, were not absorbed by red cells but could be absorbed by tumor cells expressing A or B blood-group determinants. Their reactivity was inhibited by some soluble blood-group glycoproteins. One serum (No. 4) was inhibited by A-active glycoproteins from human saliva and ovarian cyst fluids and from porcine mucosa, as well as by a polysaccharide derived from gastric cancer; it has an anti-A-like specificity. The other serum (No. 6) was inhibited by A and B glycoproteins, by a blood group precursor glycoprotein and by the same gastric cancer polysaccharide; it seems to have a wider specificity directed towards both A- and B-like structures. It is not known what caused production of these antibodies but it may be significant that they occurred in members of a family at high risk for developing colon cancer.
Studies of tobacco mosaic virus reassembly with an RNA tail blocked by a hybridised and cross-linked probe (1986)

Segments of cloned cDNA to tobacco mosaic virus RNA, 150--300-bases long, have been hybridised and cross-linked to the RNA, which has then been used for reassembly experiments. This enables the elongation reaction, which does not encapsidate the double-stranded region generated, to be stopped at specific regions along the RNA and the resulting particles to be characterised, by measuring the lengths of the rods in the electron microscope. With hybridisation to the 3'-tail the entire RNA contiguous to the nucleation region is encapsidated, from the 5'-terminus up to the modified region. When the double-stranded region is on the 5'-side of the nucleation region, the mean length of the particles corresponds to a situation in which the double-stranded region is unable to enter the central hole of the growing rod, but the 3'-tail of the RNA is completely encapsidated. The longest particles hybridised on the 5'-tail (i.e. in a class longer than the mean length) show an effect complementary to those with a 3'-block, and have lengths which correspond to encapsidation from the modified region to the 3'-terminus, despite the continued presence of the 5'-tail up the rod. In all cases where there is a remaining 5'-tail the lengths observed can only be explained if elongation has occurred substantially, or probably completely, along the 3'-tail. Hence elongation must have occurred simultaneously along both the 5' and 3'-tails of the tobacco mosaic virus RNA after initiation on the internal nucleation region.
A microarray MEMS device for biolistic delivery of vaccine and drug powders (2015)

We report a biolistic technology platform for physical delivery of particle formulations of drugs or vaccines using parallel arrays of microchannels, which generate highly collimated jets of particles with high spatial resolution. Our approach allows for effective delivery of therapeutics sequentially or concurrently (in mixture) at a specified target location or treatment area. We show this new platform enables the delivery of a broad range of particles with various densities and sizes into both in vitro and ex vivo skin models. Penetration depths of ∼1 mm have been achieved following a single ejection of 200 µg high-density gold particles, as well as 13.6 µg low-density polystyrene-based particles into gelatin-based skin simulants at 70 psi inlet gas pressure. Ejection of multiple shots at one treatment site enabled deeper penetration of ∼3 mm in vitro, and delivery of a higher dose of 1 mg gold particles at similar inlet gas pressure. We demonstrate that particle penetration depths can be optimized in vitro by adjusting the inlet pressure of the carrier gas, and dosing is controlled by drug reservoirs that hold precise quantities of the payload, which can be ejected continuously or in pulses. Future investigations include comparison between continuous versus pulsatile payload deliveries. We have successfully delivered plasmid DNA (pDNA)-coated gold particles (1.15 µm diameter) into ex vivo murine and porcine skin at low inlet pressures of ∼30 psi. Integrity analysis of these pDNA-coated gold particles confirmed the preservation of full-length pDNA after each particle preparation and jetting procedures. This technology platform provides distinct capabilities to effectively deliver a broad range of particle formulations into skin with specially designed high-speed microarray ejector nozzles.
Finite Element Simulation of Process Control for Bolt Tightening in Joints With Nonparallel Contact (2013)

In this paper, 3D finite element analysis (FEA) is used to simulate and evaluate different process control methods that are commonly used for automating the assembly of bolted joints in a mass production environment. The finite element (FE) model takes into account the thread helix angle of a fastener along with parallel and nonparallel contact surfaces under the bolt head. Simulation includes the torque-only and the torque-turn process control methods for achieving a desired level of the bolt preload at initial assembly of the joint. The torque-only process control option is simulated by applying the target torque at which the tightening process is automatically stopped. On the other hand, a torque-turn or torque-angle method is simulated by first applying a low level (threshold) torque, to the bolt head, followed by turning the bolt head by a specified angle of turn in order to achieve the desired bolt tension. The effect of variables such as thread and underhead bearing friction coefficients and bolt hole clearance is investigated. The FEA simulation provided in this study would be helpful in developing a reliable tightening strategy for joints with nonparallel bearing surfaces.
Clodronate treatment leads to microglial activation in degenerative retinopathy (2016)

Inflammation and glial activation can change the course of diabetic retinopathy (DR). The polycystic-kidney-disease (PKD)-rat shows a retinal phenotype similar to DR in which CD74 serves as a microglial activation marker. Our aim was to clarify the function of CD74+-microglia.
Two-Stage Discrete Mechanical Modelin Three-Dimensional Spacefor Garmentsimulation (2018)

Abstract This paper focuses on the better performance between the garment simulation result and the simulation speed. For simplicity and clarity, a notation “PART” is defined to indicate the areas between the garment and the human body satisfying some constraints. The discrete mechanical model can be achieved by the two-stage process. In the first stage, the garment can be divided into several PARTs constrained by the distance. In the second stage, the mechanical model of each PART is formulated with a mathematical expression. Thus, the mechanical model of the garment can be obtained. Through changing the constrained distance, the simulation result and the simulation speed can be observed. From the variable distance, a desired value can be chosen for an optimal value. The results of simulations and experiments demonstrate that the better performance can be achieved at a higher speed by saving runtime with the acceptable simulation results and the efficiency of the proposed scheme can be verified as well.
Magnetic resonance enterography is feasible and reliable in multicenter clinical trials in patients with Crohn's disease, and may help select subjects with active inflammation (2015)

Magnetic resonance enterography of high-quality and reproducibility was feasible in a global multi- centre setting, with evidence for improved selectivity over CDAI and ileocolonoscopy in identifying appropriate CD patients for inclusion in therapeutic intervention trials.