gebhart/patrepeval huggingface dataset:
Anti-SCID mouse reactivity shapes the human CD4+ T cell repertoire in hu-PBL-SCID chimeras. (1994)

Injecting human peripheral blood mononuclear cells into severe combined immunodeficient (SCID) mice results in long-term engraftment of human lymphocytes, of which > 98% are phenotypically mature, activated T cells. Here we have characterized the human T cells that populate such hu-PBL-SCID chimeras. We report that these human T cells do not mobilize Ca2+ after CD3 stimulation, i.e., their T cell receptor (TCR)-mediated signal transduction is deficient. Chimera-derived human T cells do not secrete lymphokines or undergo blastogenesis after CD3 stimulation, but proliferate in response to interleukin 2 (IL-2), defining the chimera derived human T cells as anergic. Anergy was seen in both the CD4+ and the CD8+ subpopulations. We established human T cell lines from chimeras. These T cells retained their anergic state for 1-2 mo in culture, after which they simultaneously regained the ability to mobilize Ca2+, secrete lymphokines, and to undergo blastogenesis following stimulation via the TCR. Once regaining proliferative responsiveness to CD3 stimulation, these CD4+ T cell lines displayed anti-SCID mouse reactivity and showed no specificity for recall antigens. All CD3-responsive CD4+ T cell clones obtained from such lines were SCID mouse specific, recognizing native major histocompatibility complex class II products on the murine cells. In contrast, chimera-derived human CD8+ cell lines and clones did not display detectable anti-mouse reactivity. The data show that the human T cell system in long term hu-PBL-SCID chimeras is nonfunctional due to both anergy and the limitation of the CD4+ repertoire to xenoreactive clones. The data suggest that long-term hu-PBL-SCID chimerism represents an atypical graft-versus-host reaction in which the human effector T cells become anergic in the murine environment.
Rapid prototyping polymers for microfluidic devices and high pressure injections (2011)

Multiple methods of fabrication exist for microfluidic devices, with different advantages depending on the end goal of industrial mass production or rapid prototyping for the research laboratory. Polydimethylsiloxane (PDMS) has been the mainstay for rapid prototyping in the academic microfluidics community, because of its low cost, robustness and straightforward fabrication, which are particularly advantageous in the exploratory stages of research. However, despite its many advantages and its broad use in academic laboratories, its low elastic modulus becomes a significant issue for high pressure operation as it leads to a large alteration of channel geometry. Among other consequences, such deformation makes it difficult to accurately predict the flow rates in complex microfluidic networks, change flow speed quickly for applications in stop-flow lithography, or to have predictable inertial focusing positions for cytometry applications where an accurate alignment of the optical system is critical. Recently, other polymers have been identified as complementary to PDMS, with similar fabrication procedures being characteristic of rapid prototyping but with higher rigidity and better resistance to solvents; Thermoset Polyester (TPE), Polyurethane Methacrylate (PUMA) and Norland Adhesive 81 (NOA81). In this review, we assess these different polymer alternatives to PDMS for rapid prototyping, especially in view of high pressure injections with the specific example of inertial flow conditions. These materials are compared to PDMS, for which magnitudes of deformation and dynamic characteristics are also characterized. We provide a complete and systematic analysis of these materials with side-by-side experiments conducted in our lab that also evaluate other properties, such as biocompatibility, solvent compatibility, and ease of fabrication. We emphasize that these polymer alternatives, TPE, PUMA and NOA, have some considerable strengths for rapid prototyping when bond strength, predictable operation at high pressure, or transitioning to commercialization are considered important for the application.
Analysis of Impact of Humidity and Temperature on Excimer Laser Ablation of Polyethylene Terephthalate, Polymethylmethacrylate, and Porcine Corneal Tissue (2019)

The proposed well-fitting multi-linear model can be utilized for compensation of temperature and humidity changes on ablation efficiency. Based on this model, a working window for optimum operation has been found (temperature 18°C to 28°C and relative humidity 25% to 65%) for a maximum deviation of ±2.5% in ablation efficiency in PMMA and porcine corneal tissue. Lasers Surg. Med. © 2019 The Authors. Lasers in Surgery and Medicine Published by Wiley Periodicals, Inc.
Magnetic Resonance Imaging of Inducible E-Selectin Expression in Human Endothelial Cell Culture (2001)

Covalent conjugates of the cross-linked iron oxide nanoparticles (CLIO) and high-affinity (K(d)(app) = 8.5 nM) anti-human E-selectin (CD62E) F(ab')(2) fragments were prepared and tested in vitro to establish feasibility of endothelial proinflammatory marker magnetic resonance (MR) imaging. The conjugates were obtained by using thiol-disulfide exchange reaction between 3-(2-pyridyl)propionyl-CLIO and S-acetylthioacetate-modified F(ab')(2) fragments. The purified CLIO-F(ab')(2) conjugates (average hydrodynamic diameter 40.6 nm) were used in experiments with the live human endothelial umbilical vein cells (HUVEC). Cells treated with IL-1 beta expressed E-selectin and showed a 100-200 times higher binding of CLIO particles (83-104 ng iron/million cells) than control cells. The binding resulted in a high superparamagnetism of HUVEC with the transverse water proton relaxation time (T2) decrease to 30-40 ms in cell precipitates. Cells did not bind/internalize CLIO-F(ab')(2) conjugates prepared using a control fragment or nonconjugated iron oxide particles before or after treatment with IL-1 beta. MR imaging of cells showed a highly specific T2-weighted signal darkening associated with cells treated with IL-1 beta and incubated with anti-E selectin. Demonstration of MR imaging of E-selectin expression justifies further development of MR-targeted agents for monitoring tumor vascular endothelial proliferation, angiogenesis, and atherosclerosis.
Explaining the Transition from Diffusion Limited to Reaction Limited Surface Assembly of Molecular Species through Spatial Variations (2017)

Surface assembly is often decomposed into two classes: diffusion and reaction limited processes. The transition between the two cases is complex because the dynamics are so different. In this article, we simulate, explain, and experimentally discuss the evolution of the spatial distribution for surface assemblies with diffusion limited and reaction limited processes. Explicitly, we demonstrate that diffusion limited and reaction limited processes show some temporal differences, but more importantly, we show that the spatial arrangements are different enough to discriminate between the two cases. Using fundamental properties, such as the diffusion constant, we calculate the evolution of the spatial profile and derive from physical, heuristic models the assembly rate for reaction and diffusion limited processes based on the individual particle's interactions with the surface. Finally, we confirm the spatial profile differences between diffusion and reaction limited cases by experimentally measuring the surface assembly between two molecules of similar size, but having different assembly routes. Unique to our description is that we have derived and simulated everything through the particle picture in place of ensemble descriptions such as the diffusion equation, and we show the equivalence between our heuristic formulas and those derived from the diffusion equation.
Cavity-type hypersonic phononic crystals (2012)

We report on the engineering of the phonon dispersion diagram in monodomain anodic porous alumina (APA) films through the porosity and physical state of the material residing in the nanopores. Lattice symmetry and inclusion materials are theoretically identified to be the main factors which control the hypersonic acoustic wave propagation. This involves the interaction between the longitudinal and the transverse modes in the effective medium and a flat band characteristic of the material residing in the cavities. Air and filled nanopores, therefore, display markedly different dispersion relations and the inclusion materials lead to a locally resonant structural behavior uniquely determining their properties under confinement. APA films emerge as a new platform to investigate the rich acoustic phenomena of structured composite matter.
Solid-state NMR approaches to lead halide perovskites (2019)

LRM
Study on the Electromagnetic Wave Propagation In the Parallel-Plate Waveguide with the Metamaterial ENZ Tunnel Embedded (2009)

본 논문에서는 초고주파 수동 부품의 전송 구조 내부에 전자기파의 차단 현상을 유발하는 불연속이 존재할 때 metamaterial을 이용하여 전파 특성에 변화를 유도할 수 있는 방법을 논의하고 원리를 규명한다. 특히 평행 평판 도파관 내부에 전파가 되지 않을 정도로 협소한 단면을 가진 영역의 매질이 ENZ(Epsilon Near Zero)의 metamaterial로 바뀔 때 전자기파가 진행되는, 이른바 터널링(tunneling) 조건(혹은 관통 효과)을 찾고 전자기학적 관점과 회로 관점으로 설명할 것이다. 전송선 이론에 불연속 구조는 물론 매질 변화를 고려한 평행 평판 도파관의 해석 결과를 다른 기법의 결과와 비교하여 타당성을 보이고, 이에 바탕을 두어 관통 효과 특성을 산란계수와 임피던스로 도시한다. This paper discusses how to change the electromagnetic waves' property in the cut-off causing discontinuity existing in the guiding structure of the RF passive component by using the metamaterial and elaborates on its principle. Particularly, we find and explain, from the viewpoint of electromagnetics and circuit theories, the so-called tunneling condition that when the segment with an extremely narrow cross-section leading to blockage in the parallel-plate waveguide is given the ENZ(Epsilon Near Zero) for its filling material, the wave starts to propagate through the segment. The analysis method as a transmission-line theory taking the discontinuity and material change into consideration is shown valid through the comparison with other methods for analyzing parallel-plate waveguides, and provides the illustration of the S-parameters and impedance describing the characteristics of the tunneling.
Lasing dynamics of super and sub luminal lasers (2015)

We study theoretically the lasing properties and the cavity lifetime of super and sub-luminal lasers. We find that obtaining the necessary conditions for superluminal lasing requires care and that a laser operating under these conditions can under some conditions tend towards bi-frequency lasing. In contrast, conditions for a subluminal laser are less stringent, and in most situations its steady-state properties are well predicted by the self-consistent single-frequency laser equations. We also study the relaxation time of power perturbation in super and sub-luminal lasers using a finite-difference-time-domain tool and present the impact of the lasing power, the group velocity and the dispersion properties of the cavity on the relaxation dynamic of such perturbations. For the subluminal laser, we find that the time constant changes by a factor that is close to the group index. In contrast, for the superluminal laser, we find that the time constant does not change by the factor given by the group index, and remains close to or above the value for an empty cavity. These finding may be interpreted to imply that the quantum noise limited linewidth of the subluminal laser decreases with increasing group index, while the same for the superluminal laser does not increase with decreasing group index. The implications of these findings on the sensitivity of sensors based on these lasers are discussed in details.
On Architecture Design, Congestion Notification, TCP Incast and Power Consumption in Data Centers (2012)

With rapid deployment of modern data center networks (DCNs), many problems with respect to DCN architecture, congestion notification, TCP Incast, and power consumption have been observed. In this paper, we provide a comprehensive survey of the most recent research activities in DCNs, with emphasis on the network architecture design, congestion notification algorithms, TCP Incast, and power consumption. We describe in detail the architecture of a typical modern DCN. Furthermore, we investigate the challenges of the typical tree-based hierarchical DCN architecture encountered today and the requirements for the optimal DCN architecture, and we classify the proposed DCN architectures into switch-centric topology and server-centric topology. A section is also devoted to describe some newly proposed DCN architectures. We present a brief overview of the TCP Incast problem along with previously proposed solutions. We also review the recent energy-efficient solutions to minimize the power consumption in DCNs. Finally, we outline possible future research topics in DCNs.
Temperature measurement of simulated annealing placements (2003)

One way to reduce the computational requirements of simulated annealing placement algorithms is to use a faster heuristic to replace the early phase of simulated annealing. Such systems need to know the starting temperature for the annealing phase that makes the best use of the existing structure, yet provides an appropriate amount of improvement. A method for determining the temperature of an existing placement from an analysis of the probability distribution of the change in cost function is presented. Using this view, a novel definition of equilibrium is given and the equilibrium temperature of a placement is defined. Temperatures of placements produced both by a simulated annealing and a min-cut placement algorithm are measured.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Field‐Effect Transistors: Selecting Semiconducting Single‐Walled Carbon Nanotubes with Narrow Bandgap Naphthalene Diimide‐Based Polymers (Adv. Electron. Mater. 8/2015) (2015)

In article number 1500074, Maria Antonietta Loi and co-workers describe a transistor structure where a network of semiconducting single-walled carbon nanotubes connects the source and drain gold electrodes and a naphthalene diimide-based conjugated polymer (PE-N-73) is wrapped around the tubes to keep them individualized. The sparks allocated in the junctions between the tubes in the inside cover image portray the charge hopping from one tube to the other, which is not diminished by the presence of PE-N-73.
A characterization study of highly tailorable 3D metamaterials in the thermal infrared for selective emission behaviors (2013)

The spectral behaviors of an externally-illuminated thermal infrared metamaterial were characterized through simulation and experimental measurement of the power transmittance and reflectance within the 6 - 20&mu;m range. Finite-difference time domain (FDTD) simulations in both 2-D and 3-D environments were swept over a multitude of bent dipole inclusion configurations at normal incidence angles to produce a model which exhibited a dominant electrical resonance in the long-wave infrared (IR) and increased in magnitude, bandwidth and wavelength as a function of the dipole length. Despite the appearance of fabrication defects in the measured samples, it was found the experimental data was in good agreement with the 3-D FDTD simulations, though not at all with the 2-D simulations. These introductory results indicate the dipole inclusion may behave in many ways similar to an antenna in the IR, enabling spectrally- and spatially-selective control of the emission pattern.
Determination of Phenylalanine Isotope Ratio Enrichment by Liquid Chromatography/Time-of-Flight Mass Spectrometry (2004)

The application of time-of-flight mass spectrometry to isotope ratio measurements has been limited by the relatively low dynamic range of the time-to-digital converter detectors available on commercial LC/ToF-MS systems. Here we report the measurement of phenylalanine isotope ratio enrichment by using a new LC/ToF-MS system with wide dynamic range. Underivatized phenylalanine was injected onto a C18 column directly with 0.1% formic acid/acetonitrile as the mobile phase. The optimal instrument parameters for the time-of-flight mass spectrometer were determined by tuning the instrument with a phenylalanine standard. The accuracy of the isotope enrichment measurement was determined by the injection of standard solutions with known isotope ratios ranging from 0.02% to 9.2%. A plot of the results against the theoretical values gave a linear curve with R2 of 0.9999. The coefficient of variation for the isotope ratio measurement was below 2%. The method is simple, rapid, and accurate and presents an attractive alternative to traditional GC/MS applications.
70 Years of LD-Steelmaking—Quo Vadis? (2022)

Basic Oxygen Furnace (BOF) steelmaking is, worldwide, the most frequently applied process. According to the world steel organization statistical report, 2021, it saw a total production share of 73.2%, or 1371.2 million tons per year of the world steel production in 2020. The rest is produced in Electric Arc Furnace (EAF)-based steel mills (26.3%), and only a very few open-hearth and induction furnace-based steel mills. The BOF technology remains the leading technology applied based on its undoubted advantages in productivity and liquid steel composition control. The BOF technology started as the LD process 70 years ago, with the first heat applied in November 1952 in a steel mill in Linz, Austria. The name LD was formed from the first letters of the two sites with the first industrial scale plants, Linz and Donawitz, both in Austria. The history and development of the process have been honored in multiple anniversary publications over the last few decades. Nevertheless, the focus of the steel industry worldwide is significantly changing following a social and political trend and the requirement for fossil-free energy generation and industrial production to be in accordance with the world climate targets committed to in relation to the decades leading up to 2050. Iron and steel production is one of the major polluters of climate changing greenhouse gases; it must change to renewable primary energy sources and the use of climate-neutral reduction agents. Because it is very obvious that carbon, as the main component for steel strength properties, cannot be eliminated totally from the steel production process, the question arises of where a “zero carbon” approach can lead? This paper will review the ongoing success story of the LD-process, discuss the recent technology advancements, and give an outlook on the future role of the process in the steel industry.
Nanoscale Limitations in Metal Oxide Electrocatalysts for Oxygen Evolution (2014)

Metal oxides are attractive candidates for low cost, earth-abundant electrocatalysts. However, owing to their insulating nature, their widespread application has been limited. Nanostructuring allows the use of insulating materials by enabling tunneling as a possible charge transport mechanism. We demonstrate this using TiO2 as a model system identifying a critical thickness, based on theoretical analysis, of about ∼4 nm for tunneling at a current density of ∼1 mA/cm(2). This is corroborated by electrochemical measurements on conformal thin films synthesized using atomic layer deposition (ALD) identifying a similar critical thickness. We generalize the theoretical analysis deriving a relation between the critical thickness and the location of valence band maximum relative to the limiting potential of the electrochemical surface process. The critical thickness sets the optimum size of the nanoparticle oxide electrocatalyst and this provides an important nanostructuring requirement for metal oxide electrocatalyst design.
Immaterial tip concept by light confinement (2001)

It is shown that the combination of TM polarized coherent evanescent light beams can lead to (x, y) confined light distributions. Moreover, owing to the evanescent nature of the interfering beams, the spatial distribution of the square modulus of the electric field does not vary versus the z-distance. Such an energy distribution can be used as a virtual tip, allowing the scanning of a sample without any mechanical contact with it.
Performance of MIMO: CM and semi-blind cases (2003)

We consider MIMO systems with flat fading under the quasi-static model, where the block size is on the order of tens of symbols. A relatively short block is required in mobile environments if the quasi-static model is to remain valid. To overcome the short data size, algorithms that exploit both training and signal properties are of interest. We focus on exploitation of training (semi-blind) and constant modulus (CM) signaling, and develop appropriate Cramer-Rao bounds (CRB) for these cases. The CRB provide insight into the level of training required, and show that in many scenarios increasing the number of training beyond about five symbols adds little new information. The analysis includes the impact of reduced channel rank on source estimation. The combination of semi-blind and CM is particularly informative for the short data sizes we have examined. We describe several algorithms, and compare simulated performance with the CRB on channel and source estimation. Both blind (ACMA) and semi-blind (scoring) approaches that exploit the CM property are compared. Using ACMA as a blind initialization, the scoring estimates can approach the semi-blind CM CRB.
Photo-Leakage Current of Thin-Film Transistors with ZnO Channels Formed at Various Oxygen Partial Pressures under Visible Light Irradiation (2012)

The effects of the oxygen partial pressure [p(O2)] during ZnO sputtering on the photo-leakage current of a ZnO thin-film transistor (TFT) were investigated. The photo-leakage current increased with decreasing p(O2). When the photon energy of incident light is smaller than the band-gap of the ZnO, electrons are excited from the trapped states existing near the valence band. It was found that the density of the electron traps increased when the p(O2) decreased. Moreover, the trap states were formed at ∼0.5 eV and 2.8–3.2 eV from the conduction band (EC) when the p(O2) decreased. The traps formed at (EC-E) of ∼0.5 eV affect the subthreshold characteristics in the dark state, while the traps formed at (EC-E) of 2.8–3.2 eV affect the photo-leakage current of the ZnO TFTs.
NAV‐003, a bispecific antibody targeting a unique mesothelin epitope and CD3ε with improved cytotoxicity against humoral immunosuppressed tumors (2023)

Mesothelin (MSLN) is a cell surface protein overexpressed in a number of cancer types. Several antibody- and cellular-based MSLN targeting agents have been tested in clinical trials where their therapeutic efficacy has been moderate at best. Previous studies using antibody and Chimeric Antigen Receptor-T cells (CAR-T) strategies have shown the importance of particular MSLN epitopes for optimal therapeutic response, while other studies have found that certain MSLN-positive tumors can produce proteins that can bind to subsets of IgG1-type antibodies and suppress their immune effector activities. In an attempt to develop an improved anti-MSLN targeting agent, we engineered a humanized divalent anti-MSLN/anti-CD3ε bispecific antibody that avoids suppressive factors, can target a MSLN epitope proximal to the tumor cell surface, and is capable of effectively binding, activating, and redirecting T cells to the surface of MSLN-positive tumor cells. NAV-003 has shown significantly improved tumor cell killing against lines producing immunosuppressive proteins in vitro and in vivo. Moreover, NAV-003 demonstrated good tolerability in mice and efficacy against patient-derived mesothelioma xenografts co-engrafted with human peripheral blood mononuclear cells. Together these data support the potential for NAV-003 clinical development and human proof-of-concept studies in patients with MSLN-expressing cancers.