gebhart/patrepeval huggingface dataset:
Exosomal lncRNA PVT1/VEGFA Axis Promotes Colon Cancer Metastasis and Stemness by Downregulation of Tumor Suppressor miR‐152‐3p (2021)

Exosomal PVT1 promotes colon cancer metastasis through its association with EGFR and VEGFA expression. miR-152-3p targets both PVT1 and VEGFA, and this regulatory pathway can be explored for drug development and as a prognostic biomarker.
Supplemental Figure S1 from Plasma KIM-1 Is Associated with Recurrence Risk after Nephrectomy for Localized Renal Cell Carcinoma: A Trial of the ECOG-ACRIN Research Group (E2805) (2023)

&lt;p&gt;Distribution of baseline KIM-1 values&lt;/p&gt;
Glucocorticosteroids as Dengue Therapeutics: Resolving Clinical Observations With a Primary Human Macrophage Model (2012)

© Sayce et al 2012. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. This is an Open Access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Presidential Nominating Politics in 1952, by Paul T. David, Malcolm Moos and Ralph M. Goldman (1955)

Journal Article Presidential Nominating Politics in 1952, by Paul T. David, Malcolm Moos and Ralph M. Goldman Get access Presidential Nominating Politics in 1952. Edited by Paul T. David, Malcolm Moos, and Ralph M. Goldman. Baltimore, The Johns Hopkins Press, 1954.—5 vols. xxii, 256; xi, 369; , xi, 363; xi, 344; xi, 298 pp. $17.50. David B. Truman David B. Truman Columbia University Search for other works by this author on: Oxford Academic Google Scholar Political Science Quarterly, Volume 70, Issue 2, June 1955, Pages 310–311, https://doi.org/10.2307/2145244 Published: 15 June 1955
A silicon single-electron pump with tunable electrostatic confinement (2014)

Nanoscale single-electron pumps could serve as the realization of a new quantum standard of electrical current. Here, a silicon quantum dot with tunable tunnel barriers is used as a source of quantized current. By controlling the electrostatic confinement of the dot via purposely engineered gate electrodes, we show that the stability of the pumping mechanisms can be dramatically enhanced. Our pump can produce a current in excess of 80 pA with experimentally determined relative uncertainty lower than 50 parts per million (ppm).
Developing formalin‐based fixative agents for post mortem brain MRI at 9.4 T (2021)

In vivo-like B<sub>1</sub> fields can be achieved in formalin fixatives using PVP and a low salt concentration, yielding lower T<sub>1</sub> , higher <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:msubsup><mml:mi>R</mml:mi><mml:mn>2</mml:mn><mml:mo>∗</mml:mo></mml:msubsup></mml:math> , and more paramagnetic QSM than without additives. The kinetics of <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:msubsup><mml:mi>R</mml:mi><mml:mn>2</mml:mn><mml:mo>∗</mml:mo></mml:msubsup></mml:math> allowed estimation of fixative tissue penetration.
CALS Technologies in Mechanical Engineering Using the Example of Developing Three-Dimensional Models of Typical Products (2020)

Three-dimensional models of the body parts were developed using the basic 3D modeling operations (rotation, extrusion, threading, etc.) of the T-FLEX CAD 3D CAD system, which made it possible to significantly simplify the work with assembly 3D models representing a complex structure consisting of a large number of parts. During the trial operation, the advantages of using the T-FLEX CAD 3D CAD system have been proven when working with complex 3D models. The advantages of using the program in the machine-building cluster are shown.
Protein ubiquitylation is essential for the schizont to merozoite transition in Plasmodium falciparum blood-stage development (2019)

Abstract Ubiquitylation is a common post translational modification of eukaryotic proteins and in the human malaria parasite, Plasmodium falciparum (Pf) overall ubiquitylation increases in the transition from intracellular schizont to extracellular merozoite stages in the asexual blood stage cycle. Here, we identify specific ubiquitylation sites of protein substrates in three intracellular parasite stages and extracellular merozoites; a total of 1464 sites in 546 proteins were identified (data available via ProteomeXchange with identifier PXD014998). 469 ubiquitylated proteins were identified in merozoites compared with only 160 in the preceding intracellular schizont stage, indicating a large increase in protein ubiquitylation associated with merozoite maturation. Following merozoite invasion of erythrocytes, few ubiquitylated proteins were detected in the first intracellular ring stage but as parasites matured through trophozoite to schizont stages the extent of ubiquitylation increased. We identified commonly used ubiquitylation motifs and groups of ubiquitylated proteins in specific areas of cellular function, for example merozoite pellicle proteins involved in erythrocyte invasion, exported proteins, and histones. To investigate the importance of ubiquitylation we screened ubiquitin pathway inhibitors in a parasite growth assay and identified the ubiquitin activating enzyme (UBA1 or E1) inhibitor MLN7243 (TAK-243) to be particularly effective. This small molecule was shown to be a potent inhibitor of recombinant PfUBA1, and a structural homology model of MLN7243 bound to the parasite enzyme highlights avenues for the development of P. falciparum specific inhibitors. We created a genetically modified parasite with a rapamycin-inducible functional deletion of uba1; addition of either MLN7243 or rapamycin to the recombinant parasite line resulted in the same phenotype, with parasite development blocked at the late schizont stage. These results indicate that the intracellular target of MLN7243 is UBA1, and this activity is essential for the final differentiation of schizonts to merozoites. The ubiquitylation of many merozoite proteins and their disappearance in ring stages are consistent with the idea that ubiquitylation leads to their destruction via the proteasome once their function is complete following invasion, which would allow amino acid recycling in the period prior to the parasite’s elaboration of a new food vacuole.
Advanced Power Converter for Universal and Flexible Power Management in Future Electricity Network (2007)

More âgreenâ power provided by Distributed Generation will enter into the European electricity network in the near future. In order to control the power flow and to ensure proper and secure operation of this future grid, with an increased level of the renewable power, new power electronic converters for grid connection of renewable sources will be needed. These power converters must be able to provide intelligent power management as well as ancillary services. This paper presents the overall structure and the control aspects of an advanced power converter for universal and flexible power management in the future European electricity network.
A7021 LCZ696, an Angiotensin Receptor with Neprilysin Inhibitor, Attenuates Renal Fibrosis and Glomerular Injury in a Mouse Model of Salt-sensitive Hypertension Using Angiotensin 1 Receptor Knockout Mouse (2018)

Objectives: LCZ 696 is newly created combinated medicine (valsartan and neprilysin inhibitor) and incorporated even in the guidelines for treatment of heart failure in the Europe and USA. Patients who develop aldosterone breakthrough in the course of treatment over a long period of time are attracting attention and the drug efficacy against this condition is also drawing attention. However, there are uncertainties about its mechanism, especially the protective action against organs. In this study, focusing on aldosterone breakthrough and kidney, we examined the action on the kidneys with LCZ696. Methods: AT1 receptor knock out mice were used. We started an oral administration of NaCl solution and a drugs from 7 days before administration of aldosterone. And prepared an aldosterone sustained administration model using an osmotic pump and implanted every mice and used an experimental system approximating the aldosterone breakthrough model. Mice group which administrated LCZ 696, valsartan (as a control group), no medication group, and untreated mice were prepared and blood pressure was measured. After 35 days, and the condition of the glomeruli of the kidney were evaluated and various parameters were analyzed. Results: A decrease in blood pressures were observed in LCZ 696 administered group compared valsartan administered group. As a result of pathological analysis of the kidney, glomeruli were cured and crushed in the Valsartan group, but the glomeruli in the LCZ 696 administration group remained generally normal. Conclusion: It is considered to suppress the appearance of myofibroblasts, which seems to be due to suppressing TGF-beta derived from myofibroblasts. In addition, neprilysin inhibitor has an effect of suppressing fibrosis by increasing ANP / BNP. LCZ 696 is thought to suppress fibrosis by elevating BNP.
Coaxing Confidences from an Old Friend: Probabilistic Classifications from Transformation Rule Lists (2001)

Transformation-based learning has been successfully employed to solve many natural language processing problems. It has many positive features, but one drawback is that it does not provide estimates of class membership probabilities. In this paper, we present a novel method for obtaining class membership probabilities from a transformation-based rule list classifier. Three experiments are presented which measure the modeling accuracy and cross-entropy of the probabilistic classifier on unseen data and the degree to which the output probabilities from the classifier can be used to estimate confidences in its classification decisions. The results of these experiments show that, for the task of text chunking, the estimates produced by this technique are more informative than those generated by a state-of-the-art decision tree.
Birefringence and polarization properties of a spun fiber around conductor with electrical currents (2020)

The linear birefringence is not easy to remove, since the spun fiber is used in optical fiber current sensor. We discuss the birefringence properties of a spun fiber around conductor with electrical currents, including the nonlinearity of linear birefringence and linearity of circular birefringence of the spun fiber. Theoretical results show that with the increase of load current, the linear birefringence of spun fiber will increase rapidly. By analyzing the impact of different polarization incident angle on the Poincaré sphere it is possible to obtain the influence of Faraday effect on spun fiber around conductor with electical currents in a high current generating system environment. We believe that the properties presented in this paper will be beneficial for reducing the influence of linear birefringence, as well as high accuracy fiber optic current sensors.
Thousands of microcantilevers for highly parallel and ultra-dense data storage (2003)

Ultrahigh storage densities of up to 1 Tb/in./sup 2/ or more can be achieved by using local-probe techniques to write, read back, and erase data in very thin polymer films. The thermomechanical scanning-probe-based data-storage concept, internally dubbed "millipede", combines ultrahigh density, small form factor, and high data rates. High data rates are achieved by parallel operation of large 2D arrays with thousands micro/nanomechanical cantilevers/tips that can be batch-fabricated by silicon surface-micromachining techniques. The inherent parallelism, the ultrahigh areal densities and the small form factor may open up new perspectives and opportunities for application in areas beyond those envisaged today.
The g5R (D250) Gene of African Swine Fever Virus Encodes a Nudix Hydrolase That Preferentially Degrades Diphosphoinositol Polyphosphates (2002)

The African swine fever virus (ASFV) g5R gene encodes a protein containing a Nudix hydrolase motif which in terms of sequence appears most closely related to the mammalian diadenosine tetraphosphate (Ap4A) hydrolases. However, purified recombinant g5R protein (g5Rp) showed a much wider range of nucleotide substrate specificity compared to eukaryotic Ap4A hydrolases, having highest activity with GTP, followed by adenosine 5'-pentaphosphate (p5A) and dGTP. Diadenosine and diguanosine nucleotides were substrates, but the enzyme showed no activity with cap analogues such as 7mGp3A. In common with eukaryotic diadenosine hexaphosphate (Ap6A) hydrolases, which prefer higher-order polyphosphates as substrates, g5Rp also hydrolyzes the diphosphoinositol polyphosphates PP-InsP5 and [PP]2-InsP4. A comparison of the kinetics of substrate utilization showed that the k(cat)/K(m) ratio for PP-InsP5 is 60-fold higher than that for GTP, which allows classification of g5R as a novel diphosphoinositol polyphosphate phosphohydrolase (DIPP). Unlike mammalian DIPP, g5Rp appeared to preferentially remove the 5-beta-phosphate from both PP-InsP5 and [PP]2-InsP4. ASFV infection led to a reduction in the levels of PP-InsP5, ATP and GTP by ca. 50% at late times postinfection. The measured intracellular concentrations of these compounds were comparable to the respective K(m) values of g5Rp, suggesting that one or all of these may be substrates for g5Rp during ASFV infection. Transfection of ASFV-infected Vero cells with a plasmid encoding epitope-tagged g5Rp suggested localization of this protein in the rough endoplasmic reticulum. These results suggest a possible role for g5Rp in regulating a stage of viral morphogenesis involving diphosphoinositol polyphosphate-mediated membrane trafficking.
Multivalency Beats Complexity: A Study on the Cell Uptake of Carbohydrate Functionalized Nanocarriers to Dendritic Cells (2020)

Herein, we report the synthesis of carbohydrate and glycodendron structures for dendritic cell targeting, which were subsequently bound to hydroxyethyl starch (HES) nanocapsules prepared by the inverse miniemulsion technique. The uptake of the carbohydrate-functionalized HES nanocapsules into immature human dendritic cells (hDCs) revealed a strong dependence on the used carbohydrate. A multivalent mannose-terminated dendron was found to be far superior in uptake compared to the structurally more complex oligosaccharides used.
Correlating MRI and clinical disease activity in multiple sclerosis (1995)

Magnetic resonance imaging (MRI) is being used as an outcome criterion in therapeutic trials in multiple sclerosis (MS) on the assumption that it, as a sensitive marker of biologic disease activity, could serve as a surrogate marker of disability. We evaluated the relation between MRI findings and disability in a quantitative follow-up study of 48 MS patients. Median duration of follow-up was 24 months (range, 10 to 42 months). Computer-assisted volume measurements employing a seed-growing technique yielded a standard error of measurement of 0.275 cm2. The median total area of the hyperintense lesions on the initial T2-weighted images was 8.4 cm2. The median increase was 0.76 cm2/yr (9%). In a subgroup (n = 19) with short-TR/short-TE spin-echo (SE) images, we measured the hypointense lesion load. The median total area of the lesions at entry was 0.70 cm2, with a median increase of 0.28 cm2/yr (40%). The total area of the hyperintense lesions on the initial T2-weighted images showed a weak correlation with the Expanded Disability Status Scale score at entry (Spearman rank correlation coefficient [SRCC] = 0.30; 0.02 < p < 0.05). The increase in disability showed a positive correlation (SRCC = 0.19) with the increase in hyperintense lesion load on the T2-weighted SE images, but this correlation did not reach statistical significance (p = 0.09), probably because of lack of clinical progression.(ABSTRACT TRUNCATED AT 250 WORDS)
Induction of Fracture Healing Using Fibrous Calcium Phosphate Composite Spherulites (1991)

The healing of large fractural defects is a difficult clinical problem, especially if it occurs in elderly or otherwise debilitated patients. The objective of this study is to determine if a new formulation of fibrous calcium phosphate crystals would induce fracture healing in vivo. Fibrous calcium phosphate (FCP) can be grown with unique size, shape, and surface area characteristics as a resorbable or nonresorbable, osteoconductive or osteoinductive material. In comparison with other conventional calcium phosphate particulates, FCP particles possess approximately x 100 to x 1000 more surface area. One-and-one-half centimeter sections were removed from the ulnas of 12 rabbits. Three groups had fibrous calcium phosphate spherulites (4-8 microns, 150-300 microns, 400-600 microns) mixed with collagen and a growth factor-bonding agent injected into the ulnar defect. One site per group was not treated. X-rays were obtained during the study and the percentage of the ulna defect filled in by callous was measured. The percentage was recorded as the amount of fracture healing for each site. Histologic examination of the ulnas was performed following sacrifice at 12 weeks. Fracture sites treated with fibrous calcium phosphate showed significantly greater healing (0.79 +/- 0.3) than control animals (0.36 +/- 0.1) (P less than .05, unpaired t-test) radiographically. Histologic examination showed that the spherulites remain in situ and become embedded within the new growth of fibrous tissue, collagen and new bone. Radiographically and histologically, FCP preparations appear to accelerate fracture healing by inducing new bone formation, into which they often become embedded.
Supplementary Figure 2 from Genetic Mutation of p53 and Suppression of the miR-17∼92 Cluster Are Synthetic Lethal in Non–Small Cell Lung Cancer due to Upregulation of Vitamin D Signaling (2023)

&lt;p&gt;Supplementary Figure 2. Expression and response in cells derived from a mouse model of adenocarcinoma.&lt;/p&gt;
Rewards and risks of human embryo creation: a personal view (2005)

Understanding how a single cell, the fertilised egg, can develop into all the tissues in the body has been referred to as one of the two great problems facing biology; the other is how the brain works. To be working on the early human embryo is a reward in itself. To see such knowledge applied to improve the lives of infertile couples provides special rewards. There are considerable technical challenges in these activities due to the paucity of experimental material, particularly in the human. A major intellectual challenge is to understand the mechanisms that regulate early embryo development, especially the role of signal molecules intrinsic to the embryo and those that are maternally derived. Central to this issue are notions of embryo autonomy and plasticity; the extent to which the same genotype can give rise to different phenotypes in response to environmental perturbations and the need to discover how severe such changes have to be to induce irreversible changes that can compromise the health of the offspring. Research areas that could be fruitful include stress-induced causal effects, cell signalling and the concept of 'quiet metabolism'. Managing assisted conception practices and their associated risks requires a regulatory framework to ensure the safety and efficacy of the new technologies. However, there is a danger of over-regulation, a cultural phenomenon characterised by a rise in audit and decline in trust. The challenge is to devise regulation in assisted reproduction technologies proportionate to the risks. The ultimate rewards will come from understanding how the human genome operates in functional terms. The ideal system in which to study this is the human blastocyst, an autonomous, functional group of mammalian cells.
Synthesis and biological activity of the novel nitric oxide synthase inhibitor Nω′-hydroxy-Nω-methyl-L-arginine (1994)

H. A. Moynihan, S. M. Roberts, H. Weldon, G. H. Allcock, E. E. Änggård and T. D. Warner, J. Chem. Soc., Perkin Trans. 1, 1994, 769 DOI: 10.1039/P19940000769
Application of enzymic Baeyer–Villiger oxidations of 2-substituted cycloalkanones to the total synthesis of (R)-(+)-lipoic acid (1995)

B. Adger, M. T. Bes, G. Grogan, R. McCague, S. Pedragosa-Moreau, S. M. Roberts, R. Villa, P. W. H. Wan and A. J. Willetts, J. Chem. Soc., Chem. Commun., 1995, 1563 DOI: 10.1039/C39950001563
Database replication (2010)

Replication is a key mechanism to achieve scalability and fault-tolerance in databases. Its importance has recently been further increased because of the role it plays in achieving elasticity at the database layer. In database replication, the biggest challenge lies in the trade-off between performance and consistency. A decade ago, performance could only be achieved through lazy replication at the expense of transactional guarantees. The strong consistency of eager approaches came with a high cost in terms of reduced performance and limited scalability. Postgres-R combined results from distributed systems and databases to develop a replication solution that provided both scalability and strong consistency. The use of group communication primitives with strong ordering and delivery guarantees together with optimized transaction handling (tailored locking, transferring logs instead of re-executing updates, keeping the message overhead per transaction constant) were a drastic departure from the state-of-the-art at the time. Ten years later, these techniques are widely used in a variety of contexts but particularly in cloud computing scenarios. In this paper we review the original motivation for Postgres-R and discuss how the ideas behind the design have evolved over the years.
UTILITY OF ELECTROPHYSIOLOGIC STUDIES IN THE MANAGEMENT OF TACHYCARDIA, SUDDEN DEATH, AND SYNCOPE* (1984)

The demonstrable value of EP studies for any given diagnostic or therapeutic category, in the last analysis, is largely a function of the subjects studied. Extrapolations from published data (that generally reflect a highly select patient population) to an individual patient can be fraught with error. Considerations of sensitivity and specificity must be balanced against the important need for information in patients at risk from life-threatening arrhythmias. We must never forget, however, that the EP substrate for any arrhythmia is not, as one might wish, a "black box" that should be expected to respond in a reproducible fashion to stimulation. The substrate is dynamic and subject to modification by change in autonomic tone, stretch, blood flow, basal rate, pH, electrolytes, oxygenation, and exposure to perhaps as yet undiscovered humoral mediators. The challenge to the clinical electrophysiologist is therefore not to exaggerate his efforts in one direction (i.e. programmed stimulation) while disregarding the other variables mentioned. Nor should we be disappointed and discard this approach because our expectations of an oversimplified model of arrhythmia testing are not fulfilled. Thus, in addition to careful stratification of patients, baseline studies should perhaps be carried out with more deliberate consideration of autonomic tone (exercise, isoproterenol), stretch (volume, handgrip, afterload), stress (physical and psychological), local anesthetic used, and body position. Only in this way will the scientific basis for acute and chronic EP testing be firmly established.