gebhart/patrepeval huggingface dataset:
Cloning and Expression of theEscherichia coliK1 Outer Membrane Protein A Receptor, a gp96 Homologue (2003)

Escherichia coli is one of the most common gram-negative bacteria that cause meningitis in neonates. Our previous studies have shown that outer membrane protein A (OmpA) of E. coli interacts with a 95-kDa human brain microvascular endothelial cell (HBMEC) glycoprotein, Ecgp, for invasion. Here, we report the identification of a gene that encodes Ecgp by screening of an HBMEC cDNA expression library as well as by 5' rapid amplification of cDNA ends. The sequence of the Ecgp gene shows that it is highly similar to gp96, a tumor rejection antigen-1, and contains an endoplasmic reticulum retention signal, KDEL. Overexpression of either Ecgp or gp96 in both HBMECs and CHO cells increases E. coli binding and invasion. We further show that Ecgp gene-transfected HBMECs express Ecgp on the cell surface despite the presence of the KDEL motif. Northern blot analysis of total RNA from various eukaryotic cells indicates that Ecgp is significantly expressed in HBMECs. Recombinant His-tagged Ecgp blocked E. coli invasion efficiently by binding directly to the bacteria. These results suggest that OmpA of E. coli K1 interacts with a gp96-like molecule on HBMECs for invasion.
Developing Uncertainty Quantification Strategies in Electromagnetic Problems Involving Highly Resonant Cavities (2021)

Abstract High-quality factor resonant cavities are challenging structures to model in electromagnetics owing to their large sensitivity to minute parameter changes. Therefore, uncertainty quantification (UQ) strategies are pivotal to understanding key parameters affecting the cavity response. We discuss here some of these strategies focusing on shielding effectiveness (SE) properties of a canonical slotted cylindrical cavity that will be used to develop credibility evidence in support of predictions made using computational simulations for this application.
Transcript levels of different cytokines and chemokines correlate with clinical and endoscopic activity in ulcerative colitis (2009)

From our data, we conclude that real-time PCR quantification of CXCL8, CXCL10, calgranulin B and CXCL2 in colonic biopsies is a simple and objective method for grading inflammation of intestinal mucosa in UC. CXCL8, CXCL10, calgranulin B and CXCL2 might be used as biomarkers and thus as an objective tool especially in clinical trials to evaluate anti-inflammatory and immunomodulatory regimens.
What’s Going On in Neural Constituency Parsers? An Analysis (2018)

David Gaddy, Mitchell Stern, Dan Klein. Proceedings of the 2018 Conference of the North American Chapter of the Association for Computational Linguistics: Human Language Technologies, Volume 1 (Long Papers). 2018.
WDM Ternary Content-addressable Memory for Optical Links (2023)

We propose a new optical ternary content-addressable memory design for ultra-high-speed packet routing and security analysis that leverages wavelength-division mul-tiplexing using silicon photonics microring modulators. The analysis shows that the energy consumption of the proposed optical Search Engine is as low as 134.2 fJ/bit.
Titanium(IV)-Promoted Regioselective Nucleophilic Ring-Opening Reaction of Chiral Epoxyallyl Alcohols with Acids as a Tool for Ready Access to Chiral 1,2,3-Triol Monoesters: Application to Stereoselective Total Synthesis of Macrolides (2011)

Titanium(IV)-promoted regioselective ring-opening reaction of chiral epoxy-allyl alcohols (Sharpless conditions as the key strategic step) is developed as a tool for ready access to chiral 5,6-dihydroxyoct-7-en-4-yl alkoxylates. Later, the synthetic utility of products thereof was demonstrated through the RCM based stereoselective synthesis of various natural products.
Decreasing Incidence of Postoperative Ileus following Total Knee Arthroplasty: A 17-Year Retrospective Review of 38,007 Knee Replacements at One Institution (2019)

Ileus following total knee arthroplasty is a clinically and financially significant postoperative complication that has not been extensively described in the orthopaedic joint literature. Ileus has been found to occur in 0.7 to 4.0% of patients after total joint arthroplasty. In a 17-year period (2001 fiscal year through 2017 fiscal year) at one institution, we found an incidence of 0.500% (190/38,007) following knee arthroplasty. In addition, the incidence of ileus following total knee arthroplasty (TKA) has drastically declined over this 17-year period, from 1.593% (13/816) in 2001 to 0.120% (4/3,332) in 2017. This decrease may be attributed to a reduction in narcotic use postoperatively, earlier ambulation following surgery, and reduction in length of hospital stay. Though postoperative ileus is not yet a preventable complication, recognition of risk factors may permit earlier intervention to ameliorate some of the morbidity associated with this condition.
The emerging role of SiGe BiCMOS technology in wired and wireless communications (2003)

SiGe BiCMOS technology is well suited for both wired and wireless communication areas. For the wired space, the key requirements are very high speed active devices, extremely accurate interconnect modeling (lumped elements and transmission lines), and the ability to do very highly integrated analog and mixed signal chips. With data rates at 40 Gb/s, the distinction between digital and analog circuits becomes blurred and low frequency models are no longer adequate. RF/Analog layouts and models are needed for all active devices. SiGe BiCMOS satisfies all these requirements.
Data associated with the publication: Charge trapping in polymer electrets with highly dilute blended arylamine donors (2021)

The data consists of all raw and processed data pertaining to this publication. From the abstract: The nature of and the ability to control biasing across dielectrics is a key area of research for the design of more versatile organic field-effect transistors (OFETs). One important technique to achieve such control is the inclusion of donor or acceptor molecules in the dielectric to modulate charge trapping therein. In this work, we report the effects of the additive concentration on OFETs with two different arylamines blended in polystyrene (PS) dielectrics, N,N′-diphenyl-N,N′-di-p-tolylbenzene-1,4-diamine (MPDA) and 4-anilinotriphenylamine (PATPA), as well as a comparison between PATPA/PS blends and dielectrics where PATPA was chemically tethered to the PS matrix. Dielectrics included in OFETs were subject to charging, and their charging capacity and stability were extracted from the OFET threshold voltages. For both MPDA/PS and PATPA/PS, blends with additive mole fractions as low as χ = 3 × 10–4 were sufficient to cause increases in the charge trapping relative to pure PS dielectrics. We observed a pronounced inverse relationship between the capacity and stability of blended systems at larger mole fractions (χ = 10–2) where the interadditive molecule distances became comparable to their size (∼1 nm). Differences between MPDA and PATPA behaviors were consistent with their different structures and sizes. Furthermore, we found a small effect on electronic trapping arising from the chemical tethering of PATPA to the PS matrix. This result was consistent with the changes made to the additive during the tethering process and small compared to the observed differences arising from the concentration. These results suggest that the average intermolecular separation is a key determinant of charge storage stability in blended dielectrics.
Perspectives in immunotherapy: meeting report from the immunotherapy bridge (December 2nd–3rd, 2020, Italy) (2021)

Improved understanding of tumor immunology has enabled the development of therapies that harness the immune system and prevent immune escape. Numerous clinical trials and real-world experience has provided evidence of the potential for long-term survival with immunotherapy in various types of malignancy. Recurring observations with immuno-oncology agents include their potential for clinical application across a broad patient population with different tumor types, conventional and unconventional response patterns, durable responses, and immune-related adverse events. Despite the substantial achievements to date, a significant proportion of patients still fail to benefit from current immunotherapy options, and ongoing research is focused on transforming non-responders to responders through the development of novel treatments, new strategies to combination therapy, adjuvant and neoadjuvant approaches, and the identification of biomarkers of response. These topics were the focus of the virtual Immunotherapy Bridge (December 2nd-3rd, 2020), organized by the Fondazione Melanoma Onlus, Naples, Italy, in collaboration with the Society for Immunotherapy of Cancer and are summarised in this report.
Damage of cellular material under simultaneous application of pressure and pulsed electric field (2000)

Influence of pulsed electric field (PEF) simultaneous to pressure treatment on moisture expression from fine-cut cellular raw material has been investigated. Dependencies of specific conductivity $σ$, liquid yield $Y$, instantaneous flow rate $v$ and qualitative juice characteristics at different modes of PEF treatment are discussed. Three main consolidation phases were observed in a case of mechanical expression. A unified approach is proposed for liquid yield data analysis allowing to reduce the data scattering caused by differences in the quality of samples. Simultaneous application of pressure and PEF treatment allows to reveal a passive form of electrical damage. Pressure provokes the damage of defected cells, enhances diffusion migration of moisture in porous cellular material and depresses the cell resealing processes. PEF application at a moment when a sample specific electrical conductivity reaches minimum and pressure achieves its constant value seemed to be the most optimal.
Spatial feedback effects in narrow-stripe index-guided semiconductor lasers (1997)

We demonstrate experimentally that spatial effects of feedback are important in determining the operating characteristics of index-guided semiconductor lasers. It is in fact the inflexibility of the index-guided mode that makes the laser susceptible to small misalignments of the external cavity, which can induce power variations of nearly 40% of the solitary laser power with feedback levels of less than 2%.(-17 dB). With a simple model, we calculate the modification of the feedback wavefront as a function of the external cavity length and show that the power variations are induced through changes in the cavity loss brought on by the interference of the feedback field with the field reflected off the laser facet. By experimental comparison to a partly gain-guided laser, we conclude that these large-scale variations will be significantly reduced or absent in purely gain-guided lasers.
Evaluation of a serological reference panel for Lassa virus (2020)

Background: Lassa virus (LASV) is endemic in several West African countries, causing seasonal outbreaks. While about 80% of infections are asymptomatic, it can result in severe haemorrhagic fever. The disease incidence has been increasing each year, with more than 700 confirmed cases in 2019. As identified in the WHO R&D Blueprint, there is a lack of, and therefore a need, to develop effective treatments and vaccines. The availability of an antibody reference reagent early in this process is of value, allowing assay harmonisation by calibration to the reference which is given an arbitrary unitage. This will enable the comparison of Lassa serological data reported between laboratories and at different stages of vaccine efficacy clinical trials. Currently, the Coalition for Epidemic Preparedness Innovations (CEPI) is funding the development of six vaccines against LASV. We are working with CEPI to make available LASV serological reference reagents for use by developers. Methods and materials: We received donations of sera from individuals recovered from LASV in Nigeria and Sierra Leone. Before arrival at NIBSC, samples were certified as negative for LASV. Characterisation was performed to confirm the presence of specific antibodies using the Zalgen ReLASV® pan-Lassa pre-fusion GP and NP ELISA kits and using an in-house VSV-based pseudotyped virus neutralisation assay, incorporating either a lineage II or IV LASV glycoprotein. In partnership with CEPI a feasibility study was launched, with vaccine and assay developers testing the candidate samples in a variety of assays. Results: A Lassa convalescent serum panel containing high, medium and low titer antibodies from two distinct geographical areas, have been evaluated in a feasibility study by six participants, using serological assays in use in their laboratories. Data returned has been statistically analysed to access whether the use of a common reference reagent can reduce inter-laboratory variation and harmonise results from different laboratories. Conclusion: We have established a Working Standard and Reference Panel for LASV serology for the harmonization and calibration of Lassa serological assay to support vaccine development, diagnostics and research. We are also sourcing material towards the establishment of a WHO International Standard, the highest order reference reagent, with a Collaborative Study involving laboratories worldwide.
Diamond nanophotonics (2012)

We demonstrate the coupling of single color centers in diamond to plasmonic and dielectric photonic structures to realize novel nanophotonic devices. Nanometer spatial control in the creation of single color centers in diamond is achieved by implantation of nitrogen atoms through high-aspect-ratio channels in a mica mask. Enhanced broadband single-photon emission is demonstrated by coupling nitrogen-vacancy centers to plasmonic resonators, such as metallic nanoantennas. Improved photon-collection efficiency and directed emission is demonstrated by solid immersion lenses and micropillar cavities. Thereafter, the coupling of diamond nanocrystals to the guided modes of micropillar resonators is discussed along with experimental results. Finally, we present a gas-phase-doping approach to incorporate color centers based on nickel and tungsten, in situ into diamond using microwave-plasma-enhanced chemical vapor deposition. The fabrication of silicon-vacancy centers in nanodiamonds by microwave-plasma-enhanced chemical vapor deposition is discussed in addition.
Optically tuned resonant optical reflectance filter (2008)

We describe the design, fabrication, and characterization of a narrow band tunable guided mode resonance (GMR) reflectance filter that is actuated by optically induced trans-cis isomerization of an azobenzene liquid crystal. Constructing a plastic replica-molded containment cell with a rubbed polyimide film to initially direct the liquid crystal molecular orientation parallel to the grating lines of the GMR filter, isomerization caused by exposure to a λ=532nm laser results in a −25nm shift of the resonant reflected wavelength.
Role of ‘atypical pathogens’ among adult hospitalized patients with community‐acquired pneumonia (2009)

ABSTRACT Background and objective: Agents such as Mycoplasma pneumoniae , Chlamydophila pneumoniae and Legionella pneumophila are recognized as important causes of community‐acquired pneumonia (CAP) worldwide. This study examined the role of these ‘atypical pathogens’ (AP) among adult hospitalized patients with CAP. Methods: A prospective, observational study of consecutive adult CAP (clinico‐radiological diagnosis) patients hospitalized during 2004–2005 was conducted. Causal organisms were determined using cultures, antigen testing and paired serology. Clinical/laboratory/radiological variables and outcomes were compared between different aetiologies, and a clinical prediction rule for AP was constructed. Results: There were 1193 patients studied (mean age 70.8 ± 18.0 years, men 59.3%). Causal organisms were identified in 468 (39.2%) patients: ‘bacterial’ (48.7%), ‘viral’ (26.9%), ‘AP’ (28.6%). The AP infections comprised Mycoplasma or Chlamydophila pneumoniae (97.8%) and co‐infection with bacteria/virus (30.6%). The majority of AP infections involved elderly patients (63.4%) with comorbidities (41.8%), and more than one‐third of patients were classified as ‘intermediate’ or ‘high’ risk CAP on presentation (pneumonia severity index IV–V (35.1%); CURB‐65 2–5 (42.5%)). Patients with AP infections had disease severities and outcomes similar to patients with CAP due to other organisms (oxygen therapy 29.1% vs 29.8%; non‐invasive ventilation 3.7% vs 3.3%; admission to the intensive care unit 4.5% vs 2.7%; length of hospitalization 6 day vs 7 day; 30‐day mortality: 2.2% vs 6.0%; overall P &gt; 0.05). Age &lt;65 years, female gender, fever ≥38.0°C, respiratory rate &lt;25/min, pulse rate &lt;100/min, serum sodium &gt;130 mmol/L, leucocyte count &lt;11 × 10 9 /L and Hb &lt; 11 g/dL were features associated with AP infection, but the derived prediction rule failed to reliably discriminate CAP caused by AP from bacterial CAP (area under the curve 0.75). Conclusions: M. pneumoniae and C. pneumoniae as single/co‐pathogens are important causes of severe pneumonia among older adults. No reliable clinical indicators exist, so empirical antibiotic coverage for hospitalized CAP patients may need to be considered.
Photochemical insertion of alkynes into iron complex Cp2Fe2(CO)2(.mu.-CO)2: a mechanistic study by laser flash photolysis (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhotochemical insertion of alkynes into iron complex Cp2Fe2(CO)2(.mu.-CO)2: a mechanistic study by laser flash photolysisBruce E. Bursten, Steven D. McKee, and Matthew S. PlatzCite this: J. Am. Chem. Soc. 1989, 111, 9, 3428–3429Publication Date (Print):April 1, 1989Publication History Published online1 May 2002Published inissue 1 April 1989https://pubs.acs.org/doi/10.1021/ja00191a050https://doi.org/10.1021/ja00191a050research-articleACS PublicationsRequest reuse permissionsArticle Views90Altmetric-Citations22LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Combination of aerobic exercise and an arginine, alanine, and phenylalanine mixture increases fat mobilization and ketone body synthesis (2017)

During exercise, blood levels of several hormones increase acutely. We hypothesized that consumption of a specific combination of amino acids (arginine, alanine, and phenylalanine; A-mix) may be involved in secretion of glucagon, and when combined with exercise may promote fat catabolism. Ten healthy male volunteers were randomized in a crossover study to ingest either A-mix (3 g/dose) or placebo (3 g of dextrin/dose). Thirty minutes after ingesting, each condition subsequently performed workload trials on a cycle ergometer at 50% of maximal oxygen consumption for 1 h. After oral intake of A-mix, the concentrations of plasma ketone bodies and adrenalin during and post-exercise were significantly increased. The area under the curve for glycerol and glucagon was significantly increased in the post-exercise by A-mix administration. These results suggest that pre-exercise ingestion of A-mix causes a shift of energy source from carbohydrate to fat combustion by increasing secretion of adrenalin and glucagon.
A comparison of implicit and explicit channel feedback methods for MU-MIMO WLAN systems (2013)

In this paper we compare the implicit and explicit methods of providing channel state information (CSI) to the transmitter in a multi-user multiple-input-multiple-output (MU-MIMO) system as specified in the draft specification IEEE 802.11ac [1]. The comparison is made on the basis of both overhead requirements and packet-error-rate (PER) performance. We also propose a hybrid feedback scheme that combines the explicit and implicit feedback methods thus benefiting from more frequent calibration without incurring extra overhead.
Thermodynamic and structural characterization of an optimized peptide-based inhibitor of the influenza polymerase PA-PB1 subunit interaction (2022)

Influenza virus causes severe respiratory infection in humans. Current antivirotics target three key proteins in the viral life cycle: neuraminidase, the M2 channel and the endonuclease domain of RNA-dependent-RNA polymerase. Due to the development of novel pandemic strains, additional antiviral drugs targetting different viral proteins are still needed. The protein-protein interaction between polymerase subunits PA and PB1 is one such possible target. We recently identified a modified decapeptide derived from the N-terminus of the PB1 subunit with high affinity for the C-terminal part of the PA subunit. Here, we optimized its amino acid hotspots to maintain the inhibitory potency and greatly increase peptide solubility. This allowed thermodynamic characterization of peptide binding to PA. Solving the X-ray structure of the peptide-PA complex provided structural insights into the interaction. Additionally, we optimized intracellular delivery of the peptide using a bicyclic strategy that led to improved inhibition in cell-based assays.
All-optical high speed demultiplexing with a semiconductor laser amplifier in a loop mirror configuration (1993)

A novel, purely optical technique for demultiplexing high speed time-division multiplexed data is demonstrated. The technique uses the SLALOM (semiconductor laser amplifier in a loop mirror). The data rate is not limited by the gain recovery time of the semiconductor laser amplifier. The same technique can also be used for multiplexing.
Speech Recognition at ICSI: Broadcast News and beyond (1998)

Overview of the International Computer Science Institute's efforts, in collaboration with European partners, to prepare a system to submit to a Defense Advanced Research Projects Agency and National Institute of Standards and Technology evaluation of approaches to speech recognition of broadcast news footage.
2-Acetonyl-2,4-di(hydroxy)tetrahydropyransversusγ-Pyrones: A Chemodivergent Issue for the Condensation of Acetylacetone Dianion Equivalents with α,β-Disubstituted β-Hydroxyaldehydes Leading to Potential New Synthons for Spiroketals (2002)

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