gebhart/patrepeval huggingface dataset:
Membrane Transporters Involved in the Antimicrobial Activities of Pyrithione in Escherichia coli (2021)

Pyrithione (2-mercaptopyridine-N-oxide) is a metal binding modified pyridine, the antibacterial activity of which was described over 60 years ago. The formulation of zinc-pyrithione is commonly used in the topical treatment of certain dermatological conditions. However, the characterisation of the cellular uptake of pyrithione has not been elucidated, although an unsubstantiated assumption has persisted that pyrithione and/or its metal complexes undergo a passive diffusion through cell membranes. Here, we have profiled specific membrane transporters from an unbiased interrogation of 532 E. coli strains of knockouts of genes encoding membrane proteins from the Keio collection. Two membrane transporters, FepC and MetQ, seemed involved in the uptake of pyrithione and its cognate metal complexes with copper, iron, and zinc. Additionally, the phenotypes displayed by CopA and ZntA knockouts suggested that these two metal effluxers drive the extrusion from the bacterial cell of potentially toxic levels of copper, and perhaps zinc, which hyperaccumulate as a function of pyrithione. The involvement of these distinct membrane transporters contributes to the understanding of the mechanisms of action of pyrithione specifically and highlights, more generally, the important role that membrane transporters play in facilitating the uptake of drugs, including metal–drug compounds.
Data Analysis Technology of Service Robot System for Business Improvement (2019)

Various kinds of data can be collected with service robot in business scenes and it is important to utilize such data for business improvement. In this paper, the research results of data analysis technology for business improvement as a function of operation and management system for service robot is explained. Through evaluation experiment for service robot in business exhibition, we clarified how to modify service scenario for business improvement and potential effect of business improvement is 8.1 %.
A Novel Method for Producing Custom-Made Idiotype Vaccines for Lymphoma Immunotherapy Using a Cell-Free Expression System. (2004)

Abstract Idiotype vaccines have been used as a customized immunotherapy for lymphoma. This strategy takes advantage of a unique antigen (either immunoglobulin or T cell receptor) found on the lymphoma cells of each patient. To make this personalized vaccine feasible for widespread use a production method, which is rapid and relatively inexpensive will be required. In an E. coli-based cell-free protein expression system we were able to make ScFv proteins derived from the immunoglobulins of a mouse B cell lymphoma, 38C13, in high yields. We have used this cell-free system to make 38C13 ScFv proteins fused to either the cytokine mGM-CSF or a 9 amino acid peptide derived from hIL-1b. The 9 amino acid fragment of hIL-1b was chosen because it is known to retain immunostimulatory function while lacking pyrogenicity. We were able consistently to produce these proteins in high yield and to purify them using a histadine tag at the terminus of the molecule. The ScFv portion of the molecule is folded properly, as assayed by its binding to an anti-idiotype Ab. Similarly, the mGM-CSF fused to the ScFv can be recognized by an anti-mGM-CSF Ab. The mGM-CSF portion is biologically active as determined by its ability to support the growth a mGM-CSF dependant cell line. We immunized C3H mice with either the ScFc-hIL-1b or the ScFv-mGM-CSF fusion protein and we were able to induce an idiotype-specific Ab response, which recognizes the native idiotype protein in ELISA assays as well as the 38C13 tumor as detected by flow cytometry. Most importantly, in the highly aggressive 38C13 lymphoma model system, three bi-weekly vaccinations with either fusion protein protected mice from tumor challenge with the syngeneic 38C13 tumor. These data establish that a cell-free protein expression system can work for producing individualized idiotype vaccines for lymphoma. (This work was supported by NIH grant CA34233)
Simplified, High Drug‐Loaded Formulations Containing Hydrochlorothiazide for Twin‐Screw Granulation (2017)

Abstract Simplified, high drug loaded formulations for use in twin‐screw granulation and tableting were introduced, using the plastically deforming drug ibuprofen. This concept was enlarged by application of hydrochlorothiazide, because opposed deformation behavior was expected to result in different product performances. In most instances the results differed. Critical quality attributes like granule size distribution, disintegration time, and tensile strength of tablets could be met. The study confirms the suitability of simplified formulation with differently behaving drugs for pharmaceutical product design in continuous manufacturing.
Robust Acoustic Pulling Using Chiral Surface Waves (2021)

We show that long-range and robust acoustic pulling can be achieved by using a pair of one-way chiral surface waves supported on the interface between two phononic crystals composed of spinning cylinders with equal but opposite spinning velocities embedded in water. When the chiral surface mode with a relatively small Bloch wave vector is excited, the particle located in the interface waveguide will scatter the excited surface mode to another chiral surface mode with a greater Bloch wave vector, resulting in an acoustic pulling force, irrespective of the size and material of the particle. Thanks to the backscattering immunity of the chiral surface waves against local disorders, the particle can be pulled, following a flexible trajectory, as determined by the shape of the interface. As such, this acoustic pulling scheme overcomes some of the limitations of the traditional acoustic pulling using structured beams, such as short pulling distances, straight-line type pulling, and a strong dependence on the scattering properties of the particle. Our work may also inspire the application of topological acoustics to acoustic manipulations.
Characterization of Lyt-2-, L3T4- class I-specific cytolytic clones in C3H-gld/gld mice. Implications for functions of accessory molecules and programmed development. (1987)

We report the first demonstration of Thy-1+, Lyt-2-, L3T4- MHC-specific CTL clones derived from the Lyt-2-, L3T4- subset of lymph node cells of C3H-gld/gld mice. These clones express alpha/beta heterodimeric TCRs on the cell surface and specifically recognize class I molecules on target cells. Lyt-2 and L3T4 molecules are therefore not essential for the induction, recognition, and killing of antigen-specific CTL. In addition, these studies suggest that antigen specificity development for class I structures may occur before Lyt-2 gene activation in the differentiation of T cells.
Quantifying the Impact of Non-linear Impairments on Blocking Load in Elastic Optical Networks (2014)

We quantify the effect of transmitter power on the blocking load of an elastic optical network utilizing SNR adapted modulation formats. A fair channel assignment policy is shown to give the best blocking performance.
Recommending improved configurations for complex objects with an application in travel planning (2013)

Users often configure complex objects with many possible internal choices. Recommendation engines that automatically configure such objects given user preferences and constraints, may provide much value in such cases. These applications generate appropriate recommendations based on user preferences. It is likely, though, that the user will not be able to fully express her preferences and constraints, requiring a phase of manual tuning of the recommended configuration. We suggest that following this manual revision, additional constraints and preferences can be automatically collected, and the recommended configuration can be automatically improved. Specifically, we suggest a recommender component that takes as input an initial manual configuration of a complex object, deduces certain user preferences and constraints from this configuration, and constructs an alternative configuration. We show an appealing application for our method in complex trip planning, and demonstrate its usability in a user study.
Molybdenum and Tungsten Cyclopentadienone Complexes. 1. Synthesis and Characterization (1996)

The labile complexes M(CO)3(CH3CN)3 (M = Mo, W) undergo facile oxidative addition reactions with 4-bromo-2-cyclopentenone to give M(η3-C5H5O)(CO)2(CH3CN)2Br 1a and 2. Complex 1a crystallizes in space group P21/m (No. 11) with a = 8.631 (2) Å, b = 12.852 (3) Å, c = 6.659 (2) Å, β = 109.27 (1)°, V = 697.3 (3) Å3, and Z = 2. The structure was refined to R(F) = 0.023 (F ≥ 4σF). 1 and 2 react with potassium tris(pyrazolyl)borate (K[HBpz3]), 2,2‘-bipyridyl (bipy), and bis(diphenylphosphino)methane (dppm) to give neutral η3-cyclopentenoyl complexes of types M(η3-C5H5O)(CO)2(HBpz3) 3a and 4, M(η3-C5H5O)(CO)2(bipy)Br 5 and 6, and M(η3-C5H5O)(CO)2(dppm)Br 7 and 8, respectively, in high yields. Single crystal structural studies have been carried out for complexes 3a, 4, and 8. 3a crystallizes in space group Pbca (No. 61) with a = 19.819 (3) Å, b = 13.899 (3) Å, c = 13.088 (2) Å, V = 3605 (1) Å3, and Z = 8, and the structure was refined to R(F) = 0.026 (F ≥ 4σF). 4 is isostructural with 3a and crystallizes in space group Pbca (No. 61) with a = 19.726 (6) Å, b = 13.924 (6) Å, c = 13.080 (4) Å, V = 3593 (2) Å3, and Z = 8, and the structure was refined to R(F) = 0.028 (F ≥ 4σF). 8 crystallizes in space group P1̄ (No. 2) with a = 10.143 (3) Å, b = 10.972 (4) Å, c = 13.708 (5) Å, α = 82.52 (2)°, β = 85.07 (2)° , γ = 84.77 (1)°, V = 1502.0 (9) Å3, and Z = 2. The structure was refined to R(F) = 0.029 (F ≥ 4σF). Treatment of complexes 3−8 with Ph3C+PF6- in methylene chloride solution led to facile hydride abstraction and formation of the stable cationic η4-cyclopentadienone complexes [M(η4-C5H4O)(CO)2 (HBpz3)]PF6 9a and 10, [M(η4-C5H4O)(CO)2(bipy)Br]PF6 11 and 12, and [W(η4-C5H4O)(CO)2(dppm)Br]PF6 (13). Complex 10 has been found to crystallize in space group Pcab (No. 61) with a = 18.242 (4) Å, b = 17.145 (4) Å, c = 13.188 (3) Å, V = 4125 (2) Å3, and Z = 8. The structure was refined to R(F) = 0.029 (F ≥ 4σF). 13 crystallizes in space group P21/n (No. 14) with a = 11.796 (3) Å, b = 17.512 (7) Å, c = 17.210 (5) Å, β = 93.67 (1)°, V = 3548 (2) Å3, and Z = 4. The structure was refined to R(F) = 0.040 (F ≥ 4σF).
Utility of auxiliary sensor data for speech enhancement (2013)

In recent years, data from various auxiliary acoustic and nonacoustic sensors have been used for enhancing noisy speech. These include bone-conduction microphones, surface electromyographic sensors, ultrasonic imaging of facial movements, etc. The signal from such sensors is correlated with the speech signal to varying degrees, and unlike microphone data, is typically not affected by acoustic background noise, making its use attractive for speech enhancement. In this paper, we discuss the measurement of the utility of such data from an information-theoretic perspective, and quantify the information that is shared between clean speech and the auxiliary signal, which is not present in the observed noisy speech signal. The measure is applied to simultaneously recorded air-and bone-conducted speech data.
Robust and Accurate Electric Field Sensing with Solid State Spin Ensembles (2019)

Electron spins in solids constitute remarkable quantum sensors. Individual defect centers in diamond were used to detect individual nuclear spins with a nanometer scale resolution, and ensemble magnetometers rival SQUID and vapor cell magnetometers when taking into account room-temperature operation and size. NV center spins can also detect electric field vectors, despite their weak coupling to electric fields. Here, we employ ensembles of NV center spins to measure macroscopic AC electric fields with high precision. We utilize low strain, <sup>12</sup>C enriched diamond to achieve the maximum sensitivity and tailor the spin Hamiltonian via the proper magnetic field adjustment to map out the AC electric field strength and polarization and arrive at refined electric field coupling constants. For high-precision measurements, we combine classical lock-in detection with aspects from quantum phase estimation for the effective suppression of technical noise. Eventually, this enables <i>t</i><sup>-1/2</sup> uncertainty scaling of the electric field strength over extended averaging periods, enabling us to reach a precision down to 10<sup>-7</sup> V/μm for an AC electric field with a frequency of 2 kHz and an amplitude of 0.012 V/ μm.
PP162—Thorough QT study with ponesimod, a selective S1P1 receptor modulator (2013)

Ponesimod is a selective sphingosine-1 phosphate (S1P1) receptor modulator currently in clinical development for multiple sclerosis and plaque psoriasis. Drug-induced prolongation of the QT interval can lead to torsades de pointes and sudden cardiac death. The aim of this study was to assess whether ponesimod has a negative QT/QTc effect as per ICH E14 guidance in a thorough QT/QTc study.
ChemInform Abstract: Synthesis and Biological Evaluation of Novel Bisindolylmaleimides that Inhibit Vascular Endothelial Cell Proliferation. (2002)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Preferential Transposition of the Maize Element Activator to Linked Chromosomal Locations in Tobacco (1990)

Jonathan D. G. Jones, Francine Carland, Eda Lim, Edward Ralston, Hugo K. Dooner, Preferential Transposition of the Maize Element Activator to Linked Chromosomal Locations in Tobacco, The Plant Cell, Vol. 2, No. 8 (Aug., 1990), pp. 701-707
High-resolution NMR spectroscopy of biological tissues using projected Magic Angle Spinning (2005)

High-resolution NMR spectra of materials subject toanisotropic broadening are usually obtained by rotating the sample aboutthe magic angle, which is 54.7 degrees to the static magnetic field. Inprojected Magic Angle Spinning (p-MAS), the sample is spun about twoangles, neither of which is the magic angle. This provides a method ofobtaining isotropic spectra while spinning at shallow angles. The p-MASexperiment may be used in situations where spinning the sample at themagic angle is not possible due to geometric or other constraints,allowing the choice of spinning angle to be determined by factors such asthe shape of the sample, rather than by the spin physics. The applicationof this technique to bovine tissue samples is demonstrated as a proof ofprinciple for future biological or medical applications.
Transition Metal Catalysis in Macromolecular Design (2000)

Metal-mediated (meth)acrylate polymerizations provide a good example of the versatility and importance of transition metal catalysis in polymer design. This chapter surveys several of these processes with respect to "living" behavior, experimental condition and monomer restrictions, and types of polymer architectures available. In addition to anionic methods, Group Transfer Polymerization (GTP) with silyl enolates or zirconocenes, organolanthanide- and aluminum porphyrin-initiated polymerization, and Atom Transfer Radical Polymerization (ATRP) are discussed.
All Supplementary Figures from Functional and Mechanistic Interrogation of BET Bromodomain Degraders for the Treatment of Metastatic Castration-resistant Prostate Cancer (2023)

&lt;p&gt;All Supplementary figures.&lt;/p&gt;
Possible model of an antiferroelectric twist grain boundary phase (2007)

Using x-ray and optical methods we have probed the structural organization of an antiferroelectric twist grain boundary phase (TGBC(a)) lying between the regular antiferroelectric smectic-C (SmC(a)* and the smectic-Q (SmQ) or isotropic phase. We find that the twist axis is everywhere perpendicular to the local smectic layer normal and that the helical superstructure is incommensurate with the smectic layer structure. The twist grain boundaries consist of a periodic lattice of alternating +1/2 and -1/2 dispirations, i.e., unit screw dislocations in combination with half unit disclinations. The molecular tilt plane is alternatingly parallel and perpendicular to the twist axis. We find that the optically measured tilt angle in the SmC(a)* phase is smaller than that measured by x rays, which is the opposite to what is found in the SmC* phase. This means that the core part tilts less than the end chains in the SmC(a)* phase, while it tilts more in the SmC* phase. On entering the TGB phase a clear decrease is measured in the tilt angle. This is explained by the elastic influence from the disclinations, which appear in this phase.
Error correcting alternatives in the machine recognition of speech (1990)

Because for many users the machine recognition of realistic, large vocabulary speech tasks typically produces recognition rates only in the neighborhood of 80%, the use of automatic speech recognizers (ASR) requires spotting and correcting the errors introduced during decoding. As a result, error correction is an important consideration in bringing current speech recognition technology to market. Standard methods for error correction include overwriting using a keyboard and selecting from a list of related candidate words with a mouse. In addition, a new alternative is considered: overwriting using a pressure-sensitive pen on a transparent paper-like interface (PLI) capable of recognizing handwriting online. These three alternatives are compared in terms of relative speed and ease of use, by measuring the average time that it takes to correct speech recognizer errors using each of the alternatives, and by recording the overall impression of each of the participants in the experiments. Keyboard entry was viewed as slow and distracting, because the attention must shift from screen to keyboard. Mouse selecting was faster but inappropriate for out-of-vocabulary words, such as proper nouns. Overall, PLI came across as a very viable means of correcting ASR errors, even in writer-independent mode. This is because the correct word often differs from the wrongly decoded one by only a few characters, thus enabling the use of (robust) discrete character recognition. In a typical experiment, users were able to correct all ASR errors after a maximum of two passes at each wrongly decoded word.
Identification of essential histidine residues in the active site of Escherichia coli xylose (glucose) isomerase. (1990)

Two conserved histidine residues (His-101 and His-271) appear to be essential components in the active site of the enzyme xylose (glucose) isomerase (EC 5.3.1.5). These amino acid residues were targeted for mutagenesis on the basis of sequence homology among xylose isomerases isolated from Escherichia coli, Bacillus subtilis, Ampullariella sp. strain 3876, and Streptomyces violaceus-niger. Each residue was selectively replaced by site-directed mutagenesis and shown to be essential for activity. No measurable activity was observed for any mutations replacing either His-101 or His-271. Circular dichroism measurements revealed no significant change in the overall conformation of the mutant enzymes, and all formed dimers similar to the wild-type enzyme. Mutations at His-271 could be distinguished from those at His-101, since the former resulted in a thermolabile protein whereas no significant change in heat stability was observed for the latter. Based upon these results and structural data recently reported, we speculate that His-101 is the catalytic base mediating the reaction. Replacement of His-271 may render the enzyme thermolabile, since this residue appears to be a ligand for one of the metal ions in the active site of the enzyme.
Transradial Access for Primary PCI (2010)

INTRODUCTION Primary angioplasty has been shown to improve survival as compared to thrombolysis, mainly due to restoration of TIMI flow in a larger percentage of patients (up to 90%). However, it has been described as suboptimal myocardial reperfusion in a relatively large proportion of patients (ranging between 20% and 40%) despite optimal restoration of epicardial perfusion (TIMI 3 flow) (1). ST-segment resolution is a cheap and easy method to assess myocardial reperfusion (2). The aim of this chapter is to review the current role of electrocardiogram (ECG) and ST-segment resolution in the assessment of optimal reperfusion in primary angioplasty.
A Primer on Techtile: An R&D Testbed for Distributed Communication, Sensing and Positioning (2021)

The Techtile measurement infrastructure is a multi-functional, versatile testbed for new communication and sensing technologies relying on fine-grained distributed resources. The facility enables experimental research on hyper-connected interactive environments and validation of new wireless connectivity, sensing and positioning solutions. It consists of a data acquisition and processing equipment backbone and a fabric of dispersed edge computing devices, Software-Defined Radios (SDRs), sensors, and LED sources. These bring intelligence close to the applications and can also collectively function as a massive, distributed resource. Furthermore, the infrastructure allows exploring more degrees and new types of diversity, i.e., scaling up the number of elements, introducing ‘3D directional diversity’ by deploying the distributed elements with different orientations, and ‘interface diversity’ by exploiting multiple technologies and hybrid signals (RF, acoustic, and visible light).
Figure S7 from Poly (ADP) Ribose Glycohydrolase Can Be Effectively Targeted in Pancreatic Cancer (2023)

&lt;p&gt;Supplementary Fig S7 describes western blot and RTPCR of PARG in modified shPARG cells.&lt;/p&gt;
Similarities of viral proteins to toxins that interact with monovalent cation channels (1991)

Garry, Robert F.; Kort, Jens J.*,†; Koch-Nolte, Friedrich‡; Koch, Gebhard† Author Information
Experimental investigation of dichroism sum rules for V, Cr, Mn, Fe, Co, and Ni: Influence of diffuse magnetism (1994)

Uncertainties in the application of the 〈Lz〉/〈Sz〉 sum rule to experimental spectra of V, Cr, Mn, Fe, Co, and Ni are discussed. An important contribution to these uncertainties is the possible presence of dichroism due to diffuse magnetic moments, which are known to exist in Fe, Co, and Ni.
Influence of the absorber layer thickness and rod length on the performance of three-dimensional nanorods thin film hydrogenated amorphous silicon solar cells (2013)

Performance of substrate-configured hydrogenated amorphous silicon solar cells based on ZnO nanorod arrays prepared by hydrothermal method has been investigated. The light harvest ability of three-dimensional nanorods solar cells is a compromise between the absorber layer thickness and the nanorods geometry. By optimizing the intrinsic a-Si:H absorber layer thickness from 75 to 250 nm and varying the length of the nanorods from 600 to 1800 nm, the highest energy conversion efficiency of 6.07% is obtained for the nanorods solar cell having thin absorber layer thickness of 200 nm with the rod length of 600 nm. This represents up to 28% enhanced efficiency compared to the conventional flat reference cell with similar absorber layer thickness.