gebhart/patrepeval huggingface dataset:
Rab27a Contributes to Cathepsin S Secretion in Lacrimal Gland Acinar Cells (2021)

Altered lacrimal gland (LG) secretion is a feature of autoimmune dacryoadenitis in Sjögren's syndrome (SS). Cathepsin S (CTSS) is increased in tears of SS patients, which may contribute to disease. Rab3D and Rab27a/b isoforms are effectors of exocytosis in LG, but Rab27a is poorly studied. To investigate whether Rab27a mediates CTSS secretion, we utilized quantitative confocal fluorescence microscopy of LG from SS-model male NOD and control male BALB/c mice, showing that Rab27a-enriched vesicles containing CTSS were increased in NOD mouse LG. Live-cell imaging of cultured lacrimal gland acinar cells (LGAC) transduced with adenovirus encoding wild-type (WT) mCFP-Rab27a revealed carbachol-stimulated fusion and depletion of mCFP-Rab27a-enriched vesicles. LGAC transduced with dominant-negative (DN) mCFP-Rab27a exhibited significantly reduced carbachol-stimulated CTSS secretion by 0.5-fold and β-hexosaminidase by 0.3-fold, relative to stimulated LGAC transduced with WT mCFP-Rab27a. Colocalization of Rab27a and endolysosomal markers (Rab7, Lamp2) with the apical membrane was increased in both stimulated BALB/c and NOD mouse LG, but the extent of colocalization was much greater in NOD mouse LG. Following stimulation, Rab27a colocalization with endolysosomal membranes was decreased. In conclusion, Rab27a participates in CTSS secretion in LGAC though the major regulated pathway, and through a novel endolysosomal pathway that is increased in SS.
Relationship between Molecular Structure and Temperature Dependence of Threshold Voltage in Fluorinated Liquid Crystals (1995)

We evaluate the temperature dependence of threshold voltage and physical constants (anisotropy of dielectric constants and elastic constants) by using fluorinated phenylbicyclohexane liquid crystal compounds. It is found that the positions of fluorine substituents influence the dielectric constant and its temperature dependence. A systematic relationship between the fluorine substituents and physical constants was obtained.
Reporter-Ribozyme zur Echtzeit-Analyse domänenspezifischer Interaktionen in Biomolekülen: reverse Transkriptase von HIV-1 und der Primer-Templatkomplex (2002)

Durch die Fusion eines Aptamers mit einem Ribozym wurde ein Aptazym erhalten, welches sensitiv und spezifisch die Anwesenheit der reversen Transkriptase (RT) von HIV-1 detektiert (siehe Bild). Über die Anwendung als Biosensor hinaus eignet sich die vorgestellte Fusionsverbindung zum Screening von Leitstrukturen, die an der Primer/Templat-Bindungsstelle der HIV-1-RT angreifen.
Folate-decorated, endostatin-loaded, nanoparticles for anti-proliferative chemotherapy in esophaegeal squamous cell carcinoma (2019)

This study aims to design and synthesize Endostatin (ENT)-loaded nanoparticles using Folic acid (FA) as a driver for targeted anti-proliferative chemotherapy in Esophageal Squamous Cell Carcinoma (ESCC). An ionic gelation method was employed to formulate FA-decorated, ENT-loaded nanoparticles, which were tested in vitro on KYSE-30 cells using unbiased stereological approaches. FA-ENT nanoparticles were internalized into ESCC cells with preferential binging to the nucleus and mitochondria for necrotic and apoptotic effects. Nanoparticles showed increased proliferation inhibition of 64.71% and reduced KYSE-30 cell migration of up to 74.12% when compared to the control. Positively charged spherical nanoparticles, with selective pH responsive ENT release, were further tested in vivo employing a tumor xenograft model. Tumor mass increased up to 5505.54 mm<sup>3</sup> in the control group while a substantial reduction occurred in the treatment group (native ENT, ENT-nano and FA-ENT-nano) down to 128.23 mm<sup>3</sup> (97.67%). Tumor volume was reduced from 1000.2 mm<sup>3</sup> to 567.64 mm<sup>3</sup> (43.25%) in the native ENT group, from 324.43 mm<sup>3</sup> to 190.25 mm<sup>3</sup> (41.36%) in ENT-nano group (non-targeted system), and from 1374.21 mm<sup>3</sup> to 998.67 mm<sup>3</sup> (27.33%) in FA-ENT-nano group (targeted system) following treatment. There were no significant differences in the body weight of mice treated with the nano-formulations as opposed to the control mice. FA-decorated nanoparticles for active transport of ENT into tumor cells with an enhanced in vitro and in vivo anti-proliferative efficacy in ESCC therapy were synthesized.
Materials Properties for the Simulation of Electro-Chemo-Mechanical Coupling Behavior of SOFC (2017)

Long-term reliability and durability are of great importance for the commercialization of SOFCs. Mechanical behaviors of ceramics components, including cell fracture, are necessary to be understood in order to ensure stability of SOFCs. For the multiscale simulation of the thermo-chemo-mechanical behavior, various related materials properties are indispensable. This paper reports the evaluation procedure of material properties for comprehensive analysis of chemically- and thermally induced mechanical behavior of SOFCs.
Allogeneic Rejection of High Grade Lymphoma: Evidence for Immune Escape in a Haploidentical Murine Model. (2006)

Abstract Allogeneic stem cell transplantation for high grade lymphoma typically suffers from high transplant related mortality and minimal success in achieving long term complete remission. The immunological aspects behind rejection of high grade lymphoma are poorly understood, partly due to the lack of animal models. Using a transgenic mouse lymphoma model, where the proto-oncogene c-myc is driven by parts of the immunoglobulin lambda locus representing a t(8;22) translocation as found in Burkitt’s lymphoma, we developed a haploidentical transplantation model. c-myc-lambda transgenic C57/BL6 mice were crossbred with DBA mice, giving rise to a B6D2F1 generation which develops high grade lymphoma spontaneously within the first 6 months post birth. Primary F1-lymphoma cell lines displayed low MHC class I and class II expression compared to wild type B-cells. When transferred into immune-competent, healthy C57/BL6 parental mice, F1-lymphoma cells were rejected even at high doses of 50 Mio. cells, whereas the syngeneic transfer of 10,000 cells into B6D2F1 mice resulted in 100% mortality due to lymphoma growth. Immunosuppression of C57/BL6 mice by lethal total body irradiation and transplantation of T-cell depleted bone marrow allowed F1-lymphoma to grow when animals were challenged at day +1 after bone marrow transplantation with 1 Mio. cells. Addback of 2.5 or 10 Mio. parental T-cells together with F1-lymphoma on day +1 completely prevented lymphoma growth, and resulted in long term survival for more than 60 days. When T-cell addback was delayed until day +3 or +6 after lymphoma challenge, animals died due to lymphoma progression 7–8 days later. By using “green” T-cells from GFP transgenic C57/BL6 mice we could show that spleens 7 days after T-cell addback contained only 0.5% (15% of total CD3+ cells) GFP positive T-cells, whereas up to 15% (55%–75% of total CD3+ cells) of total splenocytes were GFP positive in long term survivors that rejected lymphoma. Similar results were obtained when T-cells were labelled with CFDA. This indicates that T-cell expansion is associated with lymphoma rejection; animals that receive T-cells after lymphoma has been established do not show any T-cell expansion, even when lymphoma cells display a haplo-mismatch and mature T-cells are co-localized within the spleen. T-Cell expansion is suppressed when lymphoma cells are present in splenic tissue, indicating efficient immune escape. Similar results were observed when bone marrow and T-cells from C57BL/6 donors were transplanted into irradiated B6D2F1 mice and 1 Mio. F1-lymphoma cells were injected on day +1 post transplantation. Despite the presence of GvHD when low number of T-cells (0.5–2.0 Mio.) were used lymphoma growth was measurable and animals eventually died due to disease progression. These results point to the crucial role of pretransplant lymphoma burden for the success of allogeneic stem cell transplantation in high grade lymphoma.
Improving SIMD efficiency for parallel Monte Carlo light transport on the GPU (2011)

Monte Carlo Light Transport algorithms such as Path Tracing (PT), Bi-Directional Path Tracing (BDPT) and Metropolis Light Transport (MLT) make use of random walks to sample light transport paths. When parallelizing these algorithms on the GPU the stochastic termination of random walks results in an uneven workload between samples, which reduces SIMD efficiency. In this paper we propose to combine stream compaction and sample regeneration to keep SIMD efficiency high during random walk construction, in spite of stochastic termination. Furthermore, for BDPT and MLT, we propose to evaluate all bidirectional connections of a sample in parallel in order to balance the workload between GPU threads and improve SIMD efficiency during sample evaluation. We present efficient parallel GPU-only implementations for PT, BDPT, and MLT in CUDA. We show that our GPU implementations outperform similar CPU implementations by an order of magnitude.
Abstract 1086: Discoidin domain receptor 1 contributes to tumorigenesis through modulation of TGFBI expression (2012)

Abstract Discoidin domain receptor 1 (DDR1) is a member of the receptor tyrosine kinase family. It is activated by binding to its ligand, collagen and plays a key role in cell survival, adhesion, migration and invasion. Although DDR1 is present in several normal tissues, it is overexpressed in various cancer types, including lung, colon, ovary and breast tumors as well as in gliomas, where it is known to be associated with poor prognosis. The significance of DDR1 in cancer was illustrated using shRNA silencing, which impaired tumor cell growth, both in vitro and in vivo. Using microarray analysis of tumor cells with DDR1 knockdown, we identified an upregulation of TGFBI expression, which was subsequently confirmed at the protein level. TGFBI is a TGFβ induced extracellular matrix protein secreted by tumor cells and has been reported to act as either a tumor promoter or suppressor, depending upon tumor type. Exogenous addition of recombinant TGFBI to tumor cells inhibited clonogenic growth, recapitulating shRNA data. When grown in vivo as xenografts, the DDR1 knockdown cell lines demonstrated a similar phenotype of reduced growth and tumors exhibited an increase in TGFBI protein levels. In summary, our data suggests that DDR1 expression levels influences tumor growth in part through modulation of TGFBI expression. Ongoing studies will help to understand how DDR1 and TGFBI are linked and contribute to tumorigenesis. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 1086. doi:1538-7445.AM2012-1086
Approximate Subspace Iteration for constructing internally balanced reduced order models of unsteady aerodynamic systems (1996)

Internal balancing is a model reduction technique that has been known for many years, and has seen many successful applications. However, the computational cost associated with constructing internally balanced models rises rapidly with the size of the original (unreduced) model, and current methods based on the Schur decomposition cannot take advantage of the matrix sparsity arising from many current analysis techniques such as finite difference, finite volume, and finite element methods. A new technique, called Approximate Subspace Iteration (ASI) has been developed which calculates an approximation to the reduced-order balanced model, while only requiring sparse matrixvector operations. The new procedure makes it feasible to form balanced reduced models of systems that are beyond consideration using current methods. ASI has been applied to several large (up to 10,000 order), sparse dynamic systems based on unsteady aerodynamic models, resulting in model reduction by several orders of magnitude with negligible loss in accuracy. The reduced order models have been applied to simple aeroelastic analyses, and the results using the reduced order models are essentially identical to those using full-order aerodynamics
A robot as mobile sensor and agent in data center energy management (2011)

No abstract available.
Instrumentation for measuring functional residual capacity in small animals (1977)

A system for measuring functional residual capacity (FRC) by the dilution method was evaluated to determine its suitability for use in neonates and small animals. The system makes use of rapid, automatically triggered valves for precise closure at end expiration. Results of a theoretical error analysis and experimental in vitro tests are presented and indicate that the measuring error in FRC is related to the FRC-to-initial rebreathing bag volume ratio. With a 200-ml bag volume one standard deviation of the calculated error was 16% for a 50-ml, 9.1% for a 100-ml, and 5.8% for a 200-ml FRC.
Splitting of a Vertical Multiwalled Carbon Nanotube Carpet to a Graphene Nanoribbon Carpet and Its Use in Supercapacitors (2013)

Potassium vapor was used to longitudinally split vertically aligned multiwalled carbon nanotubes carpets (VA-CNTs). The resulting structures have a carpet of partially split MWCNTs and graphene nanoribbons (GNRs). The split structures were characterized by scanning electron microscopy, transmission electron microscopy, atomic force microscopy, Raman spectroscopy and X-ray photoelectron spectroscopy. When compared to the original VA-CNTs carpet, the split VA-CNTs carpet has enhanced electrochemical performance with better specific capacitance in a supercapacitor. Furthermore, the split VA-CNTs carpet has excellent cyclability as a supercapacitor electrode material. There is a measured maximum power density of 103 kW/kg at an energy density of 5.2 Wh/kg and a maximum energy density of 9.4 Wh/kg. The superior electrochemical performances of the split VA-CNTs can be attributed to the increased surface area for ion accessibility after splitting, and the lasting conductivity of the structure with their vertical conductive paths based on the preserved GNR alignment.
ChemInform Abstract: CARBENOID CHEMISTRY. REACTION OF PYRROLE DERIVATIVES WITH ETHYL DIAZOACETATE (1980)

Abstract Pyrrol (I) reagiert mit Diazoessigester (II) in Gegenwart von Katalysatoren zu den isomeren Substitutionsprodukten (III) und (IV).
Genetics of pancreatic neuroendocrine tumors: implications for the clinic (2015)

Pancreatic neuroendocrine tumors (PanNETs) are a common and deadly neoplasm of the pancreas. Although the importance of genetic alterations in PanNETs has been known for many years, recent comprehensive sequencing studies have greatly expanded our knowledge of neuroendocrine tumorigenesis in the pancreas. These studies have identified specific cellular processes that are altered in PanNETs, highlighted alterations with prognostic implications, and pointed to pathways for targeted therapies. In this review, we will discuss the genetic alterations that play a key role in PanNET tumorigenesis, with a specific focus on those alterations with the potential to change the way patients with these neoplasms are diagnosed and treated.
Body centric UWB communications (2006)

info:eu-repo/semantics/published
Analysis of the fine antigenic specificity of the (T,G)-A--L specific helper T cell continuous line E-9M(+). (1982)

Abstract The TCGF-dependent E-9M( + ) T cell continuous line was derived from in vitro activated T cells to (T,G)-A-L, which were further enriched for specific helper activity by incubation on antigen-pulsed splenic adherent cells. Cells and supernatants of this continuous line manifest a (T,G)-A-L specific helper activity, as assayed in an in vitro hapten-carrier antibody production system. The fine antigenic specificity of the helper effect induced by the E-9M(+) line was studied using 2 experimental approaches: A) NIP-OVA primed spleen cells were cultured with T cells of the line or its derived factor and challenged with NIP conjugated to different polypeptide antigens related to (T,G)-A-L. B) Supernatants of the E-9M(+) line were passed through immunoabsorbents built of the different synthetic polypeptide antigens, and the residual (T,G)-A-L specific helper activity was tested in the effluents and eluates recovered from the columns. Identical cross-reaction patterns were observed using both experimental systems. With the random synthetic polypeptides it was found that cells of the (T,G)-A-L specific E-9M(+) line or their derived factor completely cross-react with (Phe,G)-A-L and G-A-L and only partially cross-react with (H,G)-A-L, whereas no cross-reaction was observed with (T,G)-Pro-L. Anti-(T,G)-A-L antibodies bind well to the above-mentioned random synthetic polypeptide antigens. Using the ordered synthetic polypeptides, we showed that cells and supernatants of the E-9M(+) line cross-react with (T-T-G-G)-A-L and not with (T-G-T-G)-A--L. The order peptide T-T-G-G represents the major antigenic determinant of the randomly polymerized (T,G)-A-L molecule, and we found that anti-(T,G)-A-L antibodies are bound completely to (T-T-G-G)-A-L and only poorly cross-react with (T-G-T-G)-A-L. Thus, the fine antigenic specificity of cells and factor of the (T,G)-A~L specific E-9M( + ) helper T cell continuous line is similar, however not identical, to that of specific antibodies.
TU‐E‐141‐09: Impact of Attenuation Correction Mode On 4D PET/CT for Target Definition in Lung Cancer Patients (2013)

Purpose: The impact of attenuation correction on respiratory‐gated (4D) PET/CT images to define lung cancer targets, relative to 4D maximum intensity projection (MIP) or 3D static images, is poorly understood. Changes from 3DPET to 4DPET SUV metrics and target volumes were characterized in patients as a function of PET acquisition and CT attenuation correction (CTAC). Methods: Lung cancer patients underwent PET/CT list‐mode examinations spanning 3 bed positions. Static and respiratory phase‐gated sinograms were unlisted to generate 3DPET and 4DPET images. Emission data were attenuation‐corrected with phase‐averaged (4DAVG) or phase‐matched (4DMATCH) 4DCT and reconstructed with OSEM (2 iterations, 28 subsets, 5 mm post‐filter) on 2.0×2.0×3.3 mm3 voxel grids. PET target volumes were defined in MIM™5.6.3 using a gradient search method. Differences in SUVmax, SUVmean, SUVpeak, and defined volumes were compared between 3D, 4D MIP, and individual 4D phases by pairwise sign‐rank tests. 4DAVG and 4DMATCH CTAC‐PET image metrics were compared between respiratory phases using multi‐group non‐parametric ANOVA. Results: For 13 FDG‐PET avid lesions, SUVmax, SUVmean and SUVpeak were significantly higher in 4DMIP and 4D phase‐gated images relative to 3D images (9–11%, p&lt;0.03). 4DMIP volumes were larger than 3D and 4D phase volumes (6–14%, p&lt;0.01). 4DMATCH SUVmax was higher than 4DAVG SUVmax near end‐expiration phase and lower around peak‐inspiration phase (p=0.001). 4DMATCH SUVmean exceeded 4DAVG SUVmean at end‐expiration (p=0.02), while SUVpeak trended similarly but was insufficiently powered (p=0.06). 4D phase volumes were not significantly different between attenuation correction methods (p&gt;0.37). Conclusion: 4DPET provides improved image quantification over 3DPET. 4DPET quantification near end‐expiration appears superior with phase‐matched CTAC, but may be less reliable during phase‐gates with residual motion and phase‐sorting CT artifacts. Optimization of CTAC from patient‐specific respiratory patterns may improve robustness of 4D PET/CT for lung cancer target definition and dose painting.
Plasma postheparin diamine oxidase. Sensitive provocative test for quantitating length of acute intestinal mucosal injury in the rat. (1983)

Diamine oxidase (DAO; EC 1.4.3.6) is an enzyme found in high activity in the mature cells of the upper villus of rat small intestinal mucosa and in very much lower activity in all other tissues in the nonpregnant rat. This study was designed to determine whether a provocative test for increasing the level of plasma DAO activity by heparin administration could be used to monitor the extent and severity of acute, severe, small intestinal mucosal injury. In adult rats, small intestinal loops of varying lengths were perfused with 2,100 mosM sodium sulfate solution for 60 min to produce selective damage to villus epithelium. Plasma postheparin DAO (PHD) activity (180 min after 400 U/kg i.p. heparin) was measured 7 h after initiation of perfusion. With increasing length of intestinal mucosal injury, there was a progressive decrease in both basal and plasma PHD activity. The decrease in plasma PHD activity closely reflected the length of intestinal mucosa injured (n = 128, r = 0.86, P less than 0.001), and it was much more sensitive (threshold limit of detection = 13% of total length, range = 67 U/ml for 100% length of injury) than unstimulated basal levels of plasma DAO (threshold = 40%, range = 2.1 U/ml). Our previous data have suggested that DAO is unique among intestinal mucosal enzymes in that circulating levels can serve as a marker of mucosal injury; this study illustrates that the addition of a low-dose heparin administration enhances the use of DAO even further as a sensitive, quantitative, circulating marker for monitoring the extent of small intestinal mucosal injury in the rat.
Compressing photodetectors for long optical pulses using a lateral blooming drain structure (1993)

A compressing photodetector structure suitable for detecting continuous wave and long pulsewidth optical signals for applications such as acoustooptical signal processing and machine vision is described. Subthreshold injection of carriers over a photodetector blooming gate is used to generate a compressing transfer characteristic. The photodetector transfer characteristic is integrating at low light levels and logarithmically compressing at higher light levels. The output voltage at which logarithmic compression becomes dominant may be adjusted in a range extending below 5-nW incident optical power by varying the bias voltage difference between the photodetector blooming and imaging gates. The predictions of simulation models that were developed correlate well with the experimental measurements.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Composite energy-absorbing assembly (2023)

An energy-absorbing assembly includes a housing and a plurality of discrete energy-absorbing elements. The housing includes a first wall and a second wall. The first wall and the second wall are spaced apart from one another to at least partially define an interior compartment. Each element of the plurality of energy-absorbing elements includes a polymer and a plurality of reinforcing fibers. The plurality of energy-absorbing elements is at least partially disposed within the interior compartment and fixed to the housing. Each energy-absorbing element of the plurality of energy-absorbing elements includes an elongated hollow structure extending between a first end and a second end. Each elongated hollow structure defines a longitudinal axis extending nonparallel to at least one of the first wall and the second wall. In various alternative aspects, each energy-absorbing element may include a transverse wall.