gebhart/patrepeval huggingface dataset:
Electrical Contact Performance Degradation in Electromechanical Components (1999)

Detailed materials evaluations have been performed for MC2969 Intent Stronglink switch monitor circuit parts returned from the field out of retired weapon systems. Evaluations of local contact resistance, surface chemical composition and surface roughness and wear have been determined as a function of component level contact loop resistance testing position. Several degradation mechanisms have been identified and correlated with the component level measurements. Operational degradation produces surface smoothing and wear with each actuation of the monitor circuit, while aging degradation is observed in the segregation of contaminant species and alloy constituent elements to the surface in the stressed wear regions.
CRISPR/Cas9 and TALENs generate heritable mutations for genes involved in small RNA processing of Glycine max and Medicago truncatula (2017)

Processing of double-stranded RNA precursors into small RNAs is an essential regulator of gene expression in plant development and stress response. Small RNA processing requires the combined activity of a functionally diverse group of molecular components. However, in most of the plant species, there are insufficient mutant resources to functionally characterize each encoding gene. Here, mutations in loci encoding protein machinery involved in small RNA processing in soya bean and Medicago truncatula were generated using the CRISPR/Cas9 and TAL-effector nuclease (TALEN) mutagenesis platforms. An efficient CRISPR/Cas9 reagent was used to create a bi-allelic double mutant for the two soya bean paralogous Double-stranded RNA-binding2 (GmDrb2a and GmDrb2b) genes. These mutations, along with a CRISPR/Cas9-generated mutation of the M. truncatula Hua enhancer1 (MtHen1) gene, were determined to be germ-line transmissible. Furthermore, TALENs were used to generate a mutation within the soya bean Dicer-like2 gene. CRISPR/Cas9 mutagenesis of the soya bean Dicer-like3 gene and the GmHen1a gene was observed in the T<sub>0</sub> generation, but these mutations failed to transmit to the T<sub>1</sub> generation. The irregular transmission of induced mutations and the corresponding transgenes was investigated by whole-genome sequencing to reveal a spectrum of non-germ-line-targeted mutations and multiple transgene insertion events. Finally, a suite of combinatorial mutant plants were generated by combining the previously reported Gmdcl1a, Gmdcl1b and Gmdcl4b mutants with the Gmdrb2ab double mutant. Altogether, this study demonstrates the synergistic use of different genome engineering platforms to generate a collection of useful mutant plant lines for future study of small RNA processing in legume crops.
Direct-write scanning probe lithography: towards a desktop fab (2011)

Massively parallel scanning-probe based methods have been used to address the challenges of nanometer to millimeter scale printing for a variety of materials and mark a step towards the realization of a "desktop fab." Such tools enable simple, flexible, high-throughput, and low-cost nano- and microscale patterning, which allow researchers to rapidly synthesize and study systems ranging from nanoparticle synthesis to biological processes. We have developed a novel scanning probe-based cantilever-free printing method termed polymer pen lithography (PPL), which uses an array of elastomeric tips to transfer materials (e.g. alkanethiols, proteins, polymers) in a direct-write manner onto a variety of surfaces. This technique takes the best attributes of dip-pen nanolithography (DPN) and eliminates many of the disadvantages of contact printing. Various related techniques such as beam pen lithography (BPL), scanning probe block copolymer lithography (SPBCL), and hard-tip, soft spring lithography (HSL) are also discussed.
A Robot-Assisted Hand Exoskeletal System for Mirror Therapy Rehabilitation (2017)

This paper proposes a robotics-assisted hand exoskeleton for mirror therapy. Through the mechanical design of hand exoskeleton, the proposed structure can perform pinching and hand grasping motion under the guidance from finger motion glove. Also, framework of proposed mechatronic system has been delineated in this paper. In order to examine the usability of proposed system, trial results of the proposed system that applying on normal human to interact with actual objects are also presented in this paper.
Mode-division multiplexing LiNbO 3 modulator using directional coupler (2016)

We designed and fabricated a LiNbO 3 integrated optical modulator for mode-division multiplexing, where optical signals in two modes can be independently controlled and combined together. Mode crosstalk was smaller than −20dB in C-band.
Selective Protection and Relative Importance of the Carboxylic Acid Groups of Zaragozic Acid A for Squalene Synthase Inhibition (1994)

Chemistry that allows selective modification of the carboxylic acid groups of the squalene synthase inhibitor zaragozic acid A (1) was developed and applied to the synthesis of compounds modified at the 3-,4-,5-,3,4-,3,5-, and 4,5-positions. A key step in this procedure is the selective debenzylation by transfer hydrogenolysis in the presence of other olefinic groups. These compounds were tested in the rat squalene synthase assay and in vivo mouse model. Modification at C3 retains significant enzyme potency and enhances oral activity, indicating that C3 is not essential for squalene synthase activity. Modification at C4 and C5 results in significant loss in enzyme activity. In contrast, substitution at C3 or C4 enhances in vivo activity. Furthermore, disubstitution at the C3 and C4 positions results in additive in vivo potency.
Sky Optimization: Semantically aware image processing of skies in\n low-light photography (2020)

The sky is a major component of the appearance of a photograph, and its color\nand tone can strongly influence the mood of a picture. In nighttime\nphotography, the sky can also suffer from noise and color artifacts. For this\nreason, there is a strong desire to process the sky in isolation from the rest\nof the scene to achieve an optimal look. In this work, we propose an automated\nmethod, which can run as a part of a camera pipeline, for creating accurate sky\nalpha-masks and using them to improve the appearance of the sky. Our method\nperforms end-to-end sky optimization in less than half a second per image on a\nmobile device. We introduce a method for creating an accurate sky-mask dataset\nthat is based on partially annotated images that are inpainted and refined by\nour modified weighted guided filter. We use this dataset to train a neural\nnetwork for semantic sky segmentation. Due to the compute and power constraints\nof mobile devices, sky segmentation is performed at a low image resolution. Our\nmodified weighted guided filter is used for edge-aware upsampling to resize the\nalpha-mask to a higher resolution. With this detailed mask we automatically\napply post-processing steps to the sky in isolation, such as automatic\nspatially varying white-balance, brightness adjustments, contrast enhancement,\nand noise reduction.\n
Adaptive aberration compensation in high NA optics (2003)

Two approaches have been proposed for achieving 25-50 Gbyte capacity on one side of a 120 mm disc: using a two-element high numerical-aperture (NA) objective and using a dual-layered disc. One of the problems with these approaches is that an error in the cover-layer thickness of only a few microns can lead to serious spherical aberration. (SA). This is a particularly serious problem in dual-layered discs due to the distance between the two recording layers. Another problem is the narrow working distance (WD) of the two-element objective lens, which can result in the lens crashing onto the disc. To overcome these problems, we previously described accurate detection of and dynamic compensation for SA (Jpn. J. Appl. Phys., 40, 2001, and Proc. of SPIE, 4342, 2002) and a single-element high NA objective with a wide WD (Tech. Dig. of ISOM, 2001). In this paper, we review our related previous work and describe the latest advances.
2X-Bandwidth Burst 6T-SRAM for Memory Bandwidth Limited Workloads (2020)

A 20KB 6T-SRAM array in 10nm CMOS demonstrates 2X higher read bandwidth in burst mode operation. The doubling of bandwidth is achieved with 51% higher energy efficiency than frequency doubling and 30% better area efficiency than doubling the number of banks.
A language modeling approach to information retrieval (1998)

Article A language modeling approach to information retrieval Share on Authors: Jay M. Ponte Computer Science Department, University of Massachusetts, Amherst Computer Science Department, University of Massachusetts, AmherstView Profile , W. Bruce Croft Computer Science Department, University of Massachusetts, Amherst Computer Science Department, University of Massachusetts, AmherstView Profile Authors Info & Claims SIGIR '98: Proceedings of the 21st annual international ACM SIGIR conference on Research and development in information retrievalAugust 1998 Pages 275–281https://doi.org/10.1145/290941.291008Online:01 August 1998Publication History 1,592citation7,260DownloadsMetricsTotal Citations1,592Total Downloads7,260Last 12 Months258Last 6 weeks34 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
A broadly neutralizing germline-like human monoclonal antibody against dengue virus envelope domain III (2019)

Dengue is the most widespread vector-borne viral disease caused by dengue virus (DENV) for which there are no safe, effective drugs approved for clinical use. Here, by using sequential antigen panning of a yeast antibody library derived from healthy donors against the DENV envelop protein domain III (DIII) combined with depletion by an entry defective DIII mutant, we identified a cross-reactive human monoclonal antibody (mAb), m366.6, which bound with high affinity to DENV DIII from all four DENV serotypes. Immunogenetic analysis indicated that m366.6 is a germline-like mAb with very few somatic mutations from the closest VH and Vλ germline genes. Importantly, we demonstrated that it potently neutralized DENV both in vitro and in the mouse models of DENV infection without detectable antibody-dependent enhancement (ADE) effect. The epitope of m366.6 was mapped to the highly conserved regions on DIII, which may guide the design of effective dengue vaccine immunogens. Furthermore, as the first germline-like mAb derived from a naïve antibody library that could neutralize all four DENV serotypes, the m366.6 can be a tool for exploring mechanisms of DENV infection, and is a promising therapeutic candidate.
Integrated on-chip C-band optical spectrum analyzer using dual-ring resonators (2015)

We demonstrate an on-chip optical spectrum analyzer (OSA) using two cascade optical ring resonators. The OSA’s span is wider than 50nm and resolution is ~0.1nm. A germanium photodetector and a p-i-n modulator are integrated on the chip and used for detection.
Structural Ensemble of an Intrinsically Disordered Polypeptide (2012)

Intrinsically disordered proteins (IDPs), which play key roles in cell signaling and regulation, do not display specific tertiary structure when isolated in solution. Instead, they dynamically explore an ensemble of unfolded configurations, adopting more stable, ordered structures only after binding to their ligands. Whether ligands induce IDP structural changes upon binding or simply bind to pre-existing conformers that are populated within the IDP's structural ensemble is not well understood. Molecular simulations can provide information with the spatiotemporal resolution necessary to resolve these issues. Here, we report on the conformational ensemble of a 15-residue wild-type p53 fragment from the TAD domain and its mutant (TAD-P27L) obtained by replica exchange molecular dynamics simulation using an optimized (fully atomistic, explicit solvent) protein model and the experimental validation of the simulation results. We use a clustering method based on structural similarity to identify conformer states populated by the peptides in solution from the simulated ensemble. We show that p53 populates solution structures that strongly resemble the ligand (MDM2)-bound structure, but at the same time, the conformational free-energy landscape is relatively flat in the absence of the ligand.
<title>Structural motion segmentation for compact image sequence representation</title> (1996)

This paper addresses a problem of extraction of the structural motion information for compact image sequence representation. In order to extract a meaningful scene structure from image sequence, global motion and region shape of moving objects are taken into consideration. Firstly, intraframe segmentation is carried out with edges that are detected from zero-crossings of a wavelet transform, and local motions are estimated using a gradient-based method. Moving regions are then extracted using the local motion based on the intraframe segmentation. Secondly, moving regions are roughly separated into the region of the moving objects based on probabilistic clustering with mixture models using the optical flow and the image intensity for each region of the intraframe segmentation. Motion segmentation can finally be obtained by iterated estimation of affine motion parameters and region reassignment according to a criterion using Gauss-Newton iterative optimization algorithm.
Cloning and Characterization of Type III Iodothyronine Deiodinase from the FishOreochromis niloticus1 (1999)

Type III iodothyronine deiodinase (D3) catalyzes the inner ring deiodination (IRD) of T4 and T3 to the inactive metabolites rT3 and 3,3'-diiodothyronine (3,3'-T2), respectively. Here we describe the cloning and characterization of complementary DNA (cDNA) coding for D3 in fish (Oreochromis niloticus, tilapia). This cDNA contains 1478 nucleotides and codes for a protein of 267 amino acids, including a putative selenocysteine (Sec) residue, encoded by a TGA triplet, at position 131. The deduced amino acid sequence shows 57-67% identity with frog, chicken, and mammalian D3, 33-39% identity with frog, fish (Fundulus heteroclitus) and mammalian D2, and 30-35% identity with fish (tilapia), chicken, and mammalian D1. The 3' UTR contains a putative Sec insertion sequence (SECIS) element. Recombinant tilapia D3 (tD3) expressed in COS-1 cells and native tD3 in tilapia brain microsomes show identical catalytic activities, with a strong preference for IRD of T3 (Km approximately 20 nM). IRD of [3,5-125I]T3 by native and recombinant tD3 are equally sensitive to inhibition by substrate analogs (T3 > T4 >> rT3) and inhibitors (gold thioglucose >> iodoacetate > propylthiouracil). Northern analysis using a tD3 riboprobe shows high expression of a 1.6-kb messenger RNA in gill and brain, although D3 activity is much higher in brain than in gill. The characterization of tD3 cDNA provides new information about the structure-activity relationship of iodothyronine deiodinases and an important tool to study the regulation of thyroid hormone bioactivity in fish.
Exploring the Biomass-Derived Chemical Space Emerging from Natural Dihydrochalcones through the Single-Step Hemisynthesis of Antibacterial Balsacones (2020)

The preparation of bioactive molecules from biomass substrates is a relevant approach to replace fossil resources. The substitution patterns of natural products facilitate the access to polyfunctional molecules; yet, only few families of biosourced compounds have been valued in this way. Renewable dihydrochalcones and cinnamyl alcohols were combined in an expedient hemisynthesis of balsacones A, B, and C. These potent antibacterial compounds were prepared following a single-step Brønsted acid-catalyzed metal-free and protective-group-free Friedel–Crafts alkylation protocol. Novel analogues were also readily accessible relying on the diversity of biosourced precursors.
Post Ga-tumor imaging interval abridged: Fe-NTA (1985)

Tumor imaging agent, Ga-citrate has been hindered by the long post-injection interval required for proper imaging, inapplicable for the short lived 68-Ga. Ga bound to blood transferrin (Tf) has been reported as being responsible for slow blood clearance. Removal has been attempted by using ligand with stronger binding ability or iron ion with high affinity for Tf. Lack of satisfactory results led us to search for another criteria, the transference reaction rate of iron ion from iron chelates to Tf. Since very fast reaction rate has been reported for Fe-nitriolotriacetrate (Fe-NTA) as compared with other iron chelates (Fe-EDTA, Fe-citrate, Fe-EDDA), exploitation of reaction rate difference was conceived as for the attainment of proper target/non-target ratio within practical tenure of time. Fe-NTA on Ga-cit biodistribution was carried out in Ehrlich-tumor bearing mice under various administration modalities, time, doses. Ability of Fe-NTA to govern the reaction of Ga with Tf was comparatively tested with other Fe-chelates (Fe-citrate, Fe-EDTA, Fe-EDDA). Although preadministration lacked effect, post-injection induced very high tumor/blood ratio(1.18 versus 0.38 of control) within 1 h post Ga, at 1.1 NTA/Fe molar ratio. Iron dose was also critical. None of other chelates showed better characteristics. Validity of proposal confirmed by beautiful scintigraphicmore » images of VX-2 tumor bearing rabbit within 20-30 min. Applicability of Fe-NTA on maneuvering biodistribution of 68-Ga, 67-Ga cit is promising. Use of ion transference rate was a workable postulate in-vivo.« less
Hotspot detection using image pattern recognition based on higher-order local auto-correlation (2011)

Below 40nm design node, systematic variation due to lithography must be taken into consideration during the early stage of design. So far, litho-aware design using lithography simulation models has been widely applied to assure that designs are printed on silicon without any error. However, the lithography simulation approach is very time consuming, and under time-to-market pressure, repetitive redesign by this approach may result in the missing of the market window. This paper proposes a fast hotspot detection support method by flexible and intelligent vision system image pattern recognition based on Higher-Order Local Autocorrelation. Our method learns the geometrical properties of the given design data without any defects as normal patterns, and automatically detects the design patterns with hotspots from the test data as abnormal patterns. The Higher-Order Local Autocorrelation method can extract features from the graphic image of design pattern, and computational cost of the extraction is constant regardless of the number of design pattern polygons. This approach can reduce turnaround time (TAT) dramatically only on 1CPU, compared with the conventional simulation-based approach, and by distributed processing, this has proven to deliver linear scalability with each additional CPU.
Fine Mapping and Characterization of Candidate Genes that Control Resistance to Cercospora sojina K. Hara in Two Soybean Germplasm Accessions (2015)

Frogeye leaf spot (FLS), caused by the fungus Cercospora sojina K. Hara, may cause a significant yield loss to soybean growers in regions with a warm and humid climate. Two soybean accessions, PI 594891 and PI 594774, were identified to carry a high level of resistance similar to that conditioned by the Rcs3 gene in 'Davis'. Previously, we reported that the resistance to FLS in these two plant introductions (PIs) was controlled by a novel gene (s) on chromosome 13 that is different from Rcs3. To fine-map the novel FLS resistance gene(s) in these two PIs, F2: 3 seeds from the crosses between PI 594891 and PI 594774, and the FLS susceptible genotype 'Blackhawk' were genotyped with SNP markers that were designed based on the SoySNP50k iSelect BeadChip data to identify recombinant events and locate candidate genes. Analysis of lines possessing key recombination events helped narrow down the FLS-resistance genomic region in PI 594891 from 3.3 Mb to a 72.6 kb region with five annotated genes. The resistance gene in PI 594774 was fine-mapped into a 540 kb region that encompasses the 72.6 kb region found in PI 594891. Sequencing five candidate genes in PI 594891 identified three genes that have several mutations in the promoter, intron, 5', and 3' UTR regions. qPCR analysis showed a difference in expression levels of these genes in both lines compared to Blackhawk in the presence of C. sojina. Based on phenotype, genotype and haplotype analysis results, these two soybean accessions might carry different resistance alleles of the same gene or two different gene(s). The identified SNPs were used to develop Kompetitive Allele Specific PCR (KASP) assays to detect the resistance alleles on chromosome 13 from the two PIs for marker-assisted selection.
Binding Sites for Bacillus thuringiensis Cry2Ae Toxin on Heliothine Brush Border Membrane Vesicles Are Not Shared with Cry1A, Cry1F, or Vip3A Toxin (2011)

The use of combinations of Bacillus thuringiensis (Bt) toxins with diverse modes of action for insect pest control has been proposed as the most efficient strategy to increase target range and delay the onset of insect resistance. Considering that most cases of cross-resistance to Bt toxins in laboratory-selected insect colonies are due to alteration of common toxin binding sites, independent modes of action can be defined as toxins sharing limited or no binding sites in brush border membrane vesicles (BBMV) prepared from the target insect larvae. In this paper, we report on the specific binding of Cry2Ae toxin to binding sites on BBMV from larvae of the three most commercially relevant heliothine species, Heliothis virescens, Helicoverpa zea, and Helicoverpa armigera. Using chromatographic purification under reducing conditions before labeling, we detected specific binding of radiolabeled Cry2Ae, which allowed us to perform competition assays using Cry1Ab, Cry1Ac, Cry1Fa, Vip3A, Cry2Ae, and Cry2Ab toxins as competitors. In these assays, Cry2Ae binding sites were shared with Cry2Ab but not with the tested Cry1 or Vip3A toxins. Our data support the use of Cry2Ae toxin in combination with Cry1 or Vip3A toxins in strategies to increase target range and delay the onset of heliothine resistance.
Bridge12Technologies/2D_ODNP_Spectroscopy_DataRep: Initial data release (2021)

ODNP data for manuscript entitled: "Overhauser Dynamic Nuclear Polarizattion Enhanced Two-Dimensional Proton NMR Spectroscopy at Low Magnetic Fields" by Timohty Keller and Thorsten Maly
Motion-Aware Ghosted Views for Single Layer Occlusions in Augmented Reality (2015)

Functional realism focuses on helping users with tasks execution through an enhanced perception of the augmented scene. This work applies a common visualization technique, Ghosting, to improve depth perception in Augmented Reality scenes. Computer Vision and Image Processing techniques are used to extract natural features from a real scene, which will guide the assignment of transparency to each pixel of the virtual object, and provide the ghosting effect while blending the virtual object into the real scene. A moving object in a real scene catches users' attention. So, it is expected that natural and important visual information of the scene does not get occluded when the moving object passes over it. Because of that, the main contribution of this work is the inclusion of a motion detection technique to the scene feature analysis step of the Ghosting technique pipeline. A qualitative evaluation of the results achieved shows that the case studies of this work, in indoor and outdoor environments, using the proposed technique led to a better depth perception of the augmented scene, preserving the most relevant information for visual attention.