gebhart/patrepeval huggingface dataset:
Abstract 33: Psychiatric Conditions In Patients Undergoing Trigger Site Deactivation Surgery For Nerve Compression Headaches (2020)

Purpose: Patients seeking surgery for their nerve compression headaches often have debilitating symptoms that can affect both their functional and psychological well-being. Prior studies have shown strong associations between psychiatric conditions and migraine headaches. This correlation has not been investigated in patients undergoing surgery for headaches. Methods: One-hundred and twenty-nine patients were enrolled prospectively and completed the Patient Health Questionnaire-2 (PHQ-2) and Migraine Headache Index (MHI) surveys preoperatively and at twelve months postoperatively. Data on psychiatric comorbidities were collected both via survey and via retrospective chart review. Results: Preoperatively, 38% of patients self-reported a diagnosis of depression, while 45% of patients met PHQ-2 criteria for likely major depressive disorder (PHQ-2 score of three or greater). Twenty-seven percent of patients reported a diagnosis of generalized anxiety disorder. Patients with self-reported depression and anxiety were more likely to report a higher severity of migraine symptoms (P<0.01 and P=0.025, respectively). At one year postoperatively, patients reported a significant decrease in their PHQ-2 score (P=0.02), with 22% of patients reporting depressive symptoms, as compared to 45% preoperatively. The preoperative presence of anxiety or depression did not affect postoperative outcomes. Conclusion: There is a high prevalence of depression and generalized anxiety disorder in patients undergoing surgery for nerve compression headaches. Comorbid psychiatric conditions do not appear to affect postsurgical outcomes. However, surgery is associated with a significant decrease in depressive symptoms. Prior studies have shown that the surgical treatment of migraine headaches is associated with improved headache symptoms and functionality. This study demonstrates that surgery is also associated with improved mental health.
Single-cell proteo-genomic reference maps of the hematopoietic system enable the purification and massive profiling of precisely defined cell states (2021)

Single-cell genomics technology has transformed our understanding of complex cellular systems. However, excessive cost and a lack of strategies for the purification of newly identified cell types impede their functional characterization and large-scale profiling. Here, we have generated high-content single-cell proteo-genomic reference maps of human blood and bone marrow that quantitatively link the expression of up to 197 surface markers to cellular identities and biological processes across all main hematopoietic cell types in healthy aging and leukemia. These reference maps enable the automatic design of cost-effective high-throughput cytometry schemes that outperform state-of-the-art approaches, accurately reflect complex topologies of cellular systems and permit the purification of precisely defined cell states. The systematic integration of cytometry and proteo-genomic data enables the functional capacities of precisely mapped cell states to be measured at the single-cell level. Our study serves as an accessible resource and paves the way for a data-driven era in cytometry.
On the Combination of Spatial Diversity and Multiuser Diversity (2004)

The intention of this letter is to clarify various aspects related to the combined use of spatial diversity and multiuser diversity in a mobile network. We consider a system with one base station and K users, and study five different spatial diversity transmission modes. We show that in general, the multiuser diversity benefit is not reduced by the proper use of spatial diversity. Numerical examples are provided.
Nuclear Protein Matrix: Association with Newly Synthesized DNA (1975)

The residual structural framework of the cell nucleus termed the nuclear protein matrix, is associated with newly synthesized DNA in regenerating rat liver. One minute after rats are injected with [3-H] thymidine, more than 90 percent of the total tritium in nuclear DNA is associated with the matrix DNA although this DNA comprises only 25 percent of the total nuclear DNA. In contrast, the bulk DNA, 75 percent of total nuclear DNA, contains less than 8 percent of the total labeled DNA. The percentage of total labeled DNA associated with the bulk DNA increases for 30 minutes after injection and decreases correspondingly in the matrix DNA.
Autoactivation of matriptase in vitro: requirement for biomembrane and LDL receptor domain (2007)

In live cells, autoactivation of matriptase, a membrane-bound serine protease, can be induced by lysophospholipids, androgens, and the polyanionic compound suramin. These structurally distinct chemicals induce different signaling pathways and cellular events that somehow, in a cell type-specific manner, lead to activation of matriptase immediately followed by inhibition of matriptase by hepatocyte growth factor activator inhibitor 1 (HAI-1). In the current study, we established an analogous matriptase autoactivation system in an in vitro cell-free setting and showed that a burst of matriptase activation and HAI-1-mediated inhibition spontaneously occurred in the insoluble fractions of cell homogenates and that this in vitro activation could be attenuated by a soluble suppressive factor(s) in cytosolic fractions. Immunofluorescence staining and subcellular fractionation studies revealed that matriptase activation occurred in the perinuclear regions. Solubilization of matriptase from cell homogenates by Triton X-100 or sonication of cell homogenates completely inhibited the effect, suggesting that matriptase activation requires proper lipid bilayer microenvironments, potentially allowing appropriate interactions of matriptase zymogens with HAI-1 and other components. Matriptase activation occurred in a narrow pH range (from pH 5.2 to 7.2), with a sharp increase in activation at the transition from pH 5.2 to 5.4, and could be completely suppressed by moderately increased ionic strength. Protease inhibitors only modestly affected activation, whereas 30 nM (5 microg/ml) of anti-matriptase LDL receptor domain 3 monoclonal antibodies completely blocked activation. These atypical biochemical features are consistent with a mechanism for autoactivation of matriptase that requires protein-protein interactions but not active proteases.
<title>Developments in synchrotron x-ray computed microtomography at the National Synchrotron Light Source</title> (1999)

Last year, the X27A beamline at the National Synchrotron Light Source (NSLS) became dedicated solely to X-Ray Computed Microtomography (XCMT). This is a third-generation instrument capable of producing tomographic volumes of 1 - 2 micron resolution over a 2 - 3 mm field of view. Recent enhancements will be discussed. These have focused on two issues: the desire for real-time data acquisition and processing and the need for highly monochromatic beam (.1% energy bandpass). The latter will permit k-edge subtraction studies and will provide improved image contrast from below the Cr (6 keV) up to the Cs (36 keV) k-edge. A range of applications that benefit from these improvements will be discussed as well. These two goals are somewhat counterproductive, however; higher monochromaticity yields a lower flux forcing longer data acquisition times. To balance the two, a more efficient scintillator for X-ray conversion is being developed. Some testing of a prototype scintillator has been performed; preliminary results will be presented here. In the meantime, data reconstruction times have been reduced, and the entire tomographic acquisition, reconstruction and volume rendering process streamlined to make efficient use of synchrotron beam time. A Fast Filtered Back Transform (FFBT) reconstruction program recently developed helped to reduce the time to reconstruct a volume of 150 X 150 X 250 pixels<SUP>3</SUP> (over 5 million voxels) from the raw camera data to 1.5 minutes on a dual R10,000 CPU. With these improvements, one can now obtain a 'quick look' of a small tomographic volume (approximately 10<SUP>6</SUP> voxels) in just over 15 minutes from the start of data acquisition.
Composite electrode/electrolyte structure (2023)

Provided is an electrode fabricated from highly electronically conductive materials such as metals, metal alloys, or electronically conductive ceramics. The electronic conductivity of the electrode substrate is maximized. Onto this electrode in the green state, a green ionic (e.g., electrolyte) film is deposited and the assembly is co-fired at a temperature suitable to fully densify the film while the substrate retains porosity. Subsequently, a catalytic material is added to the electrode structure by infiltration of a metal salt and subsequent low temperature firing. The invention allows for an electrode with high electronic conductivity and sufficient catalytic activity to achieve high power density in ionic (electrochemical) devices such as fuel cells and electrolytic gas separation systems.
A pilot study on the kinetics of metabolites and microvascular cutaneous effects of nitric oxide inhalation in healthy volunteers (2019)

Both nitrite and SNO-Hb increase during NO inhalation. Nitrite increases first, followed by a more sustained increase in Hb-SNO. Nitrite and Hb-SNO could be a mobile reservoir of NO with potential implications on the systemic microvasculature.
Characterization and Analysis of a Flexural Shape Memory Alloy Actuator (2021)

Shape memory alloys (SMAs) are popular as actuators for use in soft robots due to their high work density and compatibility with miniaturized on-board batteries and power electronics. However, because SMA actuators are activated through electrical Joule heating, they exhibit poor energy efficiency and low actuator frequencies that arise from long cool-down times. Moreover, in the case of SMA wires that are subject to flexural loading, their load capacity and mechanical work output decrease exponentially with decreasing cross-sectional area. In this study, we perform analytic and numerical analyses to examine the thermal and structural design space around a particular class of flexural SMA wire actuators with the intention of increasing actuator operating frequency and actuation forces. Measurements obtained through experimental testing are consistent with theoretical studies of actuator force output and provide additional insight into the efficiency of electrical-to-mechanical energy conversion. Together, the theoretical and experimental studies provide insights that have the potential to inform SMA wire design and usage in soft robotic applications.
Launching Propagating Surface Plasmon Polaritons by a Single Carbon Nanotube Dipolar Emitter (2011)

We report on the excitation of propagating surface plasmon polaritons in thin metal films by a single emitter. Upon excitation in the visible regime, individual semiconducting single-walled carbon nanotubes are shown to act as directional near-infrared point dipole sources launching propagating surface plasmons mainly along the direction of the nanotube axis. Plasmon excitation and propagation is monitored in Fourier and real space by leakage radiation microscopy and is modeled by rigorous theoretical calculations. Coupling to plasmons almost completely reshapes the emission of nanotubes both spatially and with respect to polarization as compared to photoluminescence on a dielectric substrate.
Teleconferencing—The acoustical environment and directional transducers (1989)

The performance of teleconferencing systems are largely a function of the acoustical environment, the type, and the location of loudspeakers and microphones. Acoustical treatment in most conference rooms is limited to carpeted floors and an acoustical ceiling. For the most part, present microphones are designed to work best under close talking conditions and have omnidirectional, or first-order unidirectional characteristics. Two small experimental conference rooms have been constructed in which the effects of room parameters such as reverberation time, noise level, and discrete echo control can be independently studied. Broadband (8-kHz) directional microphones and steerable directional arrays, mostly of second order, are being investigated for a variety of acoustical environmental conditions. The easily adjustable conference rooms allow us to investigate these microphone systems with respect to their directional properties, placement, and steering algorithms. Loudspeaker directional characteristics, and advantages of stereo transmission are also under investigation. The experimental conference rooms and new directional microphones will be described and a discussion of trade-offs between major parameters for speech reception and transmission will be given.
Articles highlighted (2019)

Bitter-induced salivary proteins increase detection threshold of quinine
Prevention of Restenosis After Angioplasty: Efficacy of Antisense Strategy Against Cell Cycle Regulatory Genes (1995)

To develop an effective strategy to prevent neointimal formation after angioplasty injury, we have identified cell cycle regulatory proteins as targets for inhibition using antisense oligonucleotides (ODN). In addition, we have developed a novel intraluminal molecular delivery method that employs the protein coat of the Hemagglutinating virus of Japan (HVJ) complexed with liposomes, to enhance the efficiency of cellular uptake and the stability of antisense oligonucleotides while minimizing non-specific toxicity. Phosphorothioate ODN are complexed with liposomes and the protein coat of the inactivated HVJ. This method results in a more rapid cellular uptake and a ten-fold higher transfection efficiency of plasmids or ODN than lipofection or passive uptake methods. HVJ-liposome complex containing ODN was incubated within the rat carotid artery for 10 minutes immediately after balloon injury. First, we examined the effect of antisense cdc (cell division cycle) 2 kinase, proliferating cell nuclear antigen (PCNA), cdk (cyclin dependent kinase) 2 kinase and cyclin B1 ODN on neointimal formation after balloon injury in the rat carotid artery model. Reverse transcription PCR in antisense ODN (PCNA and cdc 2 kinase) transfected vessels showed a marked decrease in cdc 2 and PCNA gene expression as compared to that in sense control ODN transfected vessels. The measurement of DNA synthesis as assessed by bromodeoxyuridine index and DNA content revealed a decrease in antisense PCNA and cdc 2 kinase ODN treated vessels on day 4 after injury as compared to sense ODN treated vessels. Transfection with either antisense cdc 2, cdk 2 kinase and cyclin B1 ODN alone resulted in a partial inhibition of neointimal formation. We then evaluated the strategy of combined antisense ODN to achieve complete inhibition. The combination of antisense ODN directed against both cdc 2 kinase and PCNA inhibited completely neointimal formation at 2 weeks after angioplasty. Moreover, the inhibitory effect of this antisense ODN combination on neointimal formation persisted up to 8 weeks after a single transfection. The combination of antisense cdc 2 kinase and cyclin B1 ODN, or antisense cdc 2 and cdk 2 kinase ODN, also resulted in near complete inhibition of neointimal formation. The presistent inhibition of neointimal formation after balloon injury was also observed by the combination of antisense cdc 2 kinase and cyclin B1 ODN. The present study documents that a single intraluminal molecular in vivo delivery of antisense cell cycle regulatory genes ODN results in a inhibition of neointimal formation in the rat carotid balloon injury model as a model of gene therapy. Interestingly, our results reveled that multiple blockade of cell cycle regulatory genes by antisense ODN are necessary to achieve the complete inhibition of neointimal formation after angioplasty.
Influence of Electrolytes on the Electronic Transfer in Electrode Materials: Ex- and In-Situ Dielectric Spectroscopy Study between 103 and 1010 Hz (2014)

LGEP 2014 ID = 1613
Efficient MoM analysis of polarization selective surfaces using trefoil knot arrays (2003)

The electromagnetic properties of (p,q) torus knots consisting of perfectly conducting thin wires were investigated previously. The (2,3) or trefoil knot has the unique property that its forward scattering characteristics depend on the handedness of an incident circularly polarized wave. This property is conserved when placing the knots in an array. We describe an efficient method of moments (MoM) approach that is especially suited for the analysis of arrays comprised of these knots. In the MoM, solving the linear system of equations is the most time consuming part when the number of unknowns becomes large. For certain classes of problems, the use of preconditioning techniques allows us to reduce the solution time considerably. Array-type of structures can be efficiently analyzed when using the block-LU preconditioner, as outlined. Also, we present the spectral radius as a criterion for assessing the convergence of the method. Finally, we apply the block-LU method to investigate the electromagnetic scattering properties of a medium sized array of trefoil knots.
Synthesis and Biological Evaluation of the First N-Alkyl Cage Dimeric 4-Aryl-1,4-dihydropyridines as Novel Nonpeptidic HIV-1 Protease Inhibitors (1999)

A first series of novel NH and N-alkyl-substituted cage dimeric 4-aryl-1,4-dihydropyridines 3a-f has been synthesized and evaluated as HIV-1 protease inhibitors in in vitro assays. While the NH and N-methyl derivatives 3a,b,e,f were almost inactive with IC(50) values of about 200 microM, the N-Benzyl compounds exhibited stronger activity with an IC(50) value of 16.2 microM for the presently best compound 3c. The type of HIV-1 protease inhibition of these novel inhibitors was characterized as competitive. With the increase of observed activity from NH and N-methyl derivatives to N-benzyl compounds, respectively, the binding mode may correspond to that of cyclic and azacyclic ureas showing hydrophobic interactions of the four aromatic residues to the S1/S1' and S2/S2' regions of HIV-1 protease.
Corticoid Binding in Mammary Tissue Slices from Lactating Cows (1976)

Mammary tissue slices (from lactating Holstein cows) incubated at 37 C with various concentrations of either hydrogen-3 cortisol or hydrogen-3 dexamethasone bound both hormones with high affinity [dissociation constants (Kd) approximately equal to 10-10M]. There were approximately 2900 and 3800 total high affinity binding sites per mammary cell for cortisol and dexamethasone, respectively. In addition, a major nonspecific component bound cortisol and dexamethasone and was unsaturable. Unlabeled cortisol and dexamethasone reduced binding of hydrogen-3 cortisol and dexamethasone whereas unlabeled 17beta-estradiol, testosterone, and progesterone were without effect. Incubation of tissue slices at 4 C reduced the total number of high affinity binding sites for cortisol by about 56% compared with similar measurements at 37 C. However, dissociation constants for the high affinity components were similar at both temperatures (Kd approximately equal to 10-10M). Macromolecules which specifically bound cortisol in the 700 X g supernatant and precipitate tissue fractions were isolated by gel filtration chromatography. Enzyme digestion experiments and treatment with p-chloromercuribenzoate indicated that the macromolecules binding cortisol were proteins. Thin-layer chromatography of bound hydrogen-3 cortisol in the 700 X g supernatant indicated that the majority of bound radioactivity was authentic cortisol. We conclude that fresh mammary tissue from lactating cows possesses protein which specifically binds corticoids with high affinity.
Atomen auf den Zahn gefühlt: Dynamische Rasterkraftmikroskopie (2002)

Das Rasterkraftmikroskop hat gegenüber dem Rastertunnelmikroskop den Vorteil, dass es auch Oberflächen von nicht leitenden Materialien abbilden kann. Die moderne dynamische Rasterkraftmikroskopie erreicht im Nicht-Kontakt-Modus die Auflösung einzelner Atome und Fehlstellen. Das wurde an verschiedenen anorganischen und organischen Materialien demonstriert. Die dynamische Rasterkraftmikroskopie kann darüber hinaus auch Kräfte an Oberflächen auf der atomaren Skala ausmessen. Wegen dieser Vorteile wird sie voraussichtlich für die Oberflächenphysik wichtiger werden als das Rastertunnelmikroskop
A Born Educator and Researcher [Guest Editorial] (2014)

The articles in this special section present the life and work of Yannis Tsividis, a reknown circuit designer who revolutionized MOS transistor modeling.
P4‐201: Beneficial catalytic autoimmunity to beta‐amyloid peptide (2012)

We previously reported human catalytic autoantibodies to amyloid β peptide (Aβ). We hypothesize that recognition of electrophilic amyloid epitopes by nucleophilic autoantibodies is an innate immune function that is recruited for catalytic clearance of amyloid deposits associated with aging and Alzheimer's disease (AD). Aβ cleavage was measured by HPLC, acid precipitation, mass spectroscopy or electrophoresis. Electrophilic Aβ (E-Aβ) was prepared by carbonylation with the lipid peroxidation end products 4-hydroxynonenal (HNE)/malonaldehyde (MDA) or phosphonate diester insertion. Covalent immune complexes were analyzed by SDS-electrophoresis. Aβ1-42 aggregates were identified by antibody or Thioflavin-T staining. IgM from healthy human sera, the first antibody class produced during B cell differentiation, catalyzed Aβ cleavage at rates superior to IgGs. Preferential Aβ cleavage by IgMs was also observed for antibodies from the sera and cerebrospinal fluid from patients with AD. Two Aβ cleaving antibody fragments were isolated from a phage library, a heterodimeric V L -V L construct (2E6) and a single domain V L construct (5D3). Treatment with antibody 2E6 induced disappearance of oligomeric and fibrillar Aβ. Intracranial antibody injection in Aβ-overexpressing transgenic mice cleared the Aβ plaques. Traditional antibodies bind antigens at complementarity determining regions (CDRs). The Aβ cleaving antibodies contained CDRs with no or minimum mutations acquired by antigen-driven diversification. Deleting the CDRs did not attenuate Aβ cleavage by antibody 2E6, but the catalytic activity was lost by replacing the framework regions (FRs) with corresponding FRs from a non-catalytic antibody. The FRs are evolutionarily conserved segments important for innate recognition of B cell superantigens without requirement for adaptive immune processes. From protease inhibitor and epitope mapping studies, the catalytic mechanism entails noncovalent binding at the Aβ C terminus followed by nucleophilic peptide bond cleavage. Antibody 2E6 reacted covalently with an electrophilic phosphonate-containing Aβ analog and the naturally-occurring Aβ-HNE/Aβ-MDA analogs (E-Aβ). Monoclonal murine antibodies (MAbs) that cleaved Aβ at low substrate concentrations were identified by immunization with non-electrophilic Aβ. A subset of MAbs induced by immunization with E-Aβ cleaved Aβ robustly. Amplification of the innate noncovalent recognition and catalytic functions of antibodies driven by age/disease-associated Aβ accumulation can remove toxic amyloid deposits.
Spatial and temporal characterisation of pesticide contamination and the ecotoxic impact in an agricultural Jura river (2018)

scientifiques de niveau recherche, publis ou non, manant des tablissements d'enseignement et de recherche franais ou trangers, des laboratoires publics ou privs.