gebhart/patrepeval huggingface dataset:
Primary Lung Dendritic Cell Cultures to Assess Efficacy of Spectinamide-1599 Against Intracellular Mycobacterium tuberculosis (2018)

There is an urgent need to treat tuberculosis (TB) quickly, effectively and without side effects. <i>Mycobacterium tuberculosis</i> (Mtb), the causative organism of TB, can survive for long periods of time within macrophages and dendritic cells and these intracellular bacilli are difficult to eliminate with current drug regimens. It is well established that Mtb responds differentially to drug treatment depending on its extracellular and intracellular location and replicative state. In this study, we isolated and cultured lung derived dendritic cells to be used as a screening system for drug efficacy against intracellular mycobacteria. Using mono- or combination drug treatments, we studied the action of spectinamide-1599 and pyrazinamide (antibiotics targeting slow-growing bacilli) in killing bacilli located within lung derived dendritic cells. Furthermore, because IFN-γ is an essential cytokine produced in response to Mtb infection and present during TB chemotherapy, we also assessed the efficacy of these drugs in the presence and absence of IFN-γ. Our results demonstrated that monotherapy with either spectinamide-1599 or pyrazinamide can reduce the intracellular bacterial burden by more than 99.9%. Even more impressive is that when TB infected lung derived dendritic cells are treated with spectinamide-1599 and pyrazinamide in combination with IFN-γ a strong synergistic effect was observed, which reduced the intracellular burden below the limit of detection. We concluded that IFN-γ activation of lung derived dendritic cells is essential for synergy between spectinamide-1599 and pyrazinamide.
64 kbit Ferroelectric-Gate-Transistor-Integrated NAND Flash Memory with 7.5 V Program and Long Data Retention (2012)

A 64 kbit (kb) one-transistor-type ferroelectric memory array was fabricated and characterized. Pt/SrBi2Ta2O9/Hf–Al–O/Si ferroelectric-gate field-effect transistors (FeFETs) were used as the memory cells. The gate length and width were 5 and 5 µm, respectively. The array design was based on NAND flash memory organized as 8 word lines × 32 blocks × 256 bit lines. Erase, program, and nondestructive-read operations were demonstrated in every block. Threshold-voltage (Vth) reading of all the 64 kb memory cells showed a clear separation between their all-erased and all-programmed states. A checkerboard pattern was also programmed in a block and the two distinguishable Vth distributions were read out. The Vth retention of a block of 2 kb memory cells showed no significant degradation after two days.
Quality of Vision in Eyes After Selective Lamellar Keratoplasty (2012)

With the increasing popularity of selective lamellar keratoplasty procedures, it is important to characterize the optical differences among penetrating keratoplasty (PK), deep anterior lamellar keratoplasty (DALK), and Descemet stripping automated endothelial keratoplasty (DSAEK). The impact of optical differences on the visual acuity (VA) in eyes after keratoplasty is significant. Quantitative evaluation of corneal higher-order aberrations (HOAs) of anterior and posterior surfaces using a rotating Scheimpflug-based corneal topographer, showed that eyes that undergo DSAEK have lower anterior corneal HOAs compared with eyes subjected to PK or DALK. In DSAEK, the anterior corneal surfaces are not replaced, which is in contrast to PK or DALK, where they are replaced. Through evaluation of corneal scatter with a densitometry program associated with the rotating Scheimpflug-based corneal topographer, 3 characteristic patterns of corneal scatter were found in eyes that had undergone keratoplasty. Investigation of the impact of corneal HOAs and corneal scatter on VA after keratoplasty showed that the VA was correlated significantly with corneal scatter.
The Five-Minute Rule 20 Years Later: and How Flash Memory Changes the Rules (2008)

In 1987, Jim Gray and Gianfranco Putzolu published their now-famous five-minute rule for trading off memory and I/O capacity. Their calculation compares the cost of holding a record (or page) permanently in memory with the cost of performing disk I/O each time the record (or page) is accessed, using appropriate fractions of prices for RAM chips and disk drives. The name of their rule refers to the break-even interval between accesses. If a record (or page) is accessed more often, it should be kept in memory; otherwise, it should remain on disk and read when needed.
Molecular features of BRCA1/2 and PALB2 mutation associated pancreatic cancer (PAC). (2014)

206 Background: Several clinical reports have indicated increased sensitivity to DNA damaging agents and PARP inhibitors in patients with PAC arising on a background of known BRCA1/2 or PALB2 germline mutation. While the mechanism of tumorigenesis in these cancer remains unclear, it appears likely that BRCA1/2germline mutations can predispose to PAC via 2 mechanisms, only one of which requires loss of the second allele. Determination as to whether biallelic inactivation of these genes is present in the tumor may have important therapeutic implications in predicting sensitivity to PARP inhibitors and other strategies targeting DNA repair. Methods: Following approval by the IRB and human bio specimen utilization committee at MSKCC, full exon sequencing of coding regions of 28 genes including BRCA1, 2 and PALB2 using exon capture by hybridization and next generation sequencing was performed on DNA extracted from 135 PAC samples. In addition, samples were analyzed for the presence of BRCA1 promotor methylation, KRAS hotspot mutations, and genome wide loss of heterozygosity (LOH) as an exploratory assessment of number and length of LOH as a marker of homologous repair deficiency / genomic instability. All patients underwent surgery for PAC at MSKCC after year 2000. Patients were selected on the basis of survival and included if they suffered a cancer-specific death within 1 year of resection or survived at least 30 months. Results: 7 tumors had mutations in BRCA2, 1 in BRCA1 and 1 in PALB2. Of 4 BRCA2 mutated samples evaluated for LOH at the BRCA2 locus, 2 demonstrated LOH while 2 did not. There was no LOH at PALB2 in the sample where a mutation was identified. Activating KRAS mutations were identified in BRCA2 mutated tumors with and without LOH. Methylation of the BRCA1promotor regions was not identified. Conclusions: The frequency of BRCA1/2 mutation carriers was as anticipated in this population. Biallelic loss of BRCA2 or PALB2 are not required for pancreatic tumorigenesis. The benefit of PARP inhibitors in BRCA1 / 2 mutated PAC may be limited to tumors with LOH of these genes. Inactivation of BRCA1 through promoter methylation is not identified in sporadic PAC.
Predicting Anthracycline Benefit: TOP2A and CEP17—Not Only but Also (2015)

This prospectively planned individual-patient pooled analysis of patient cases from five adjuvant trials confirms that patients whose tumors harbor either CEP17 duplication or TOP2A aberrations, but not HER2 amplification, benefit from adjuvant anthracycline chemotherapy.
Kinetic Studies on Microsomal Glucose Dehydrogenase in Rat Liver (1975)

Glucose dehydrogenase from rat liver microsomes was found to react not only with glucose as a substrate but also with glucose 6-phosphate, 2-deoxyglucose 6-phosphate and galactose 6-phosphate. The relative maximum activity of this enzyme was 29% for glucose 6-phosphate, 99% for 2-deoxyglucose 6-phosphate, and 25% for galactose 6-phosphate, compared with 100% for glucose with NADP. The enzyme could utilize either NAD or NADP as a coenzyme. Using polyacrylamide gradient gel electrophoresis, we were able to detect several enzymatically active bands by incubation of the gels in a tetrazolium assay mixture. Each band had different Km values for the substrates (3.0 x 10(-5)M glucose 6-phosphate with NADP to 2.4M glucose with NAD) and for coenzymes (1.3 x 10(-6)M NAD with galactose 6-phosphate to 5.9 x 10(-5)M NAD with glucose). Though glucose 6-phosphate and galactose 6-phosphate reacted with glucose dehydrogenase, they inhibited the reaction of this enzyme only when either glucose or 2-deoxyglucose 6-phosphate was used as a substrate. The Ki values for glucose 6-phosphate with glucose as substrate were 4.0 x 10(-6)M with NAD, and 8.4 x 10(-6)M with NADP; for galactose 6-phosphate they were 6.7 x10(-6)M with NAD and 6.0 x 10(-6)M with NADP. The Ki values for glucose 6-phosphate with 2-deoxyglucose 6-phosphate as substrate were 6.3 x 10(-6)M with NAD and 8.9 x 10(-6)M with NADP; and for galactose 6-phosphate, 8.0 x 10(-6)M with NAD and 3.5 x 10(-6)M with NADP. Both NADH and NADPH inhibited glucose dehydrogenase when the corresponding oxidized coenzymes were used (Ki values: 8.0 x 10(-5)M by NADH and 9.1 x 10(-5)M by NADPH), while only NADPH inhibited cytoplasmic glucose 6-phosphate dehydrogenase (Ki: 2.4 x 10(-5)M). The results indicate that glucose dehydrogenase cannot directly oxidize glucose in vivo, but it might play a similar role to glucose 6-phosphate dehydrogenase. The differences in the kinetics of glucose dehydrogenase and glucose 6-phosphate dehydrogenase show that glucose 6-phosphate and galactose 6-phosphate could be metabolized in quite different ways in the microsomes and cytoplasm of rat liver.
Shannon Number of an Image and Structural Information Capacity in Volume Holography (1980)

Abstract A new approach for specifying structural information in volume holography, using a grating vector uncertainty relation and a modified Ewald's construction, is proposed and the optimization of the Shannon number of an image is given.
Direct Conversion of Syngas to Higher Alcohols via Tandem Integration of Fischer–Tropsch Synthesis and Reductive Hydroformylation (2022)

The selective conversion of syngas to higher alcohols is an attractive albeit elusive route in the quest for effective production of chemicals from alternative carbon resources. We report the tandem integration of solid cobalt Fischer-Tropsch and molecular hydroformylation catalysts in a one-pot slurry-phase process. Unprecedented selectivities (>50 wt %) to C<sub>2+</sub> alcohols are achieved at CO conversion levels >70 %, alongside negligible CO<sub>2</sub> side-production. The efficient overall transformation is enabled by catalyst engineering, bridging gaps in operation temperature and intrinsic selectivity which have classically precluded integration of these reactions in a single conversion step. Swift capture of 1-olefin Fischer-Tropsch primary products by the molecular hydroformylation catalyst, presumably within the pores of the solid catalyst is key for high alcohol selectivity. The results underscore that controlled cooperation between solid aggregate and soluble molecular metal catalysts, which pertain to traditionally dichotomic realms of heterogeneous and homogeneous catalysis, is a promising blueprint toward selective conversion processes.
A Fully-Automated Pipeline for Detection and Segmentation of Liver\n Lesions and Pathological Lymph Nodes (2017)

We propose a fully-automated method for accurate and robust detection and\nsegmentation of potentially cancerous lesions found in the liver and in lymph\nnodes. The process is performed in three steps, including organ detection,\nlesion detection and lesion segmentation. Our method applies machine learning\ntechniques such as marginal space learning and convolutional neural networks,\nas well as active contour models. The method proves to be robust in its\nhandling of extremely high lesion diversity. We tested our method on volumetric\ncomputed tomography (CT) images, including 42 volumes containing liver lesions\nand 86 volumes containing 595 pathological lymph nodes. Preliminary results\nunder 10-fold cross validation show that for both the liver lesions and the\nlymph nodes, a total detection sensitivity of 0.53 and average Dice score of\n$0.71 \\pm 0.15$ for segmentation were obtained.\n
Metathesis of Hex‐1‐ene ion Ionic Liquids Catalyzed by WCl6. (2005)

Abstract For Abstract see ChemInform Abstract in Full Text.
In-Situ Probe of Gate Dielectric-Semiconductor Interfacial Order in Organic Transistors: Origin and Control of Large Performance Sensitivities (2012)

Organic thin film transistor (OTFT) performance is highly materials interface-dependent, and dramatic performance enhancements can be achieved by properly modifying the semiconductor/gate dielectric interface. However, the origin of these effects is not well understood, as this is a classic "buried interface" problem that has traditionally been difficult to address. Here we address the question of how n-octadecylsilane (OTS)-derived self-assembled monolayers (SAMs) on Si/SiO(2) gate dielectrics affect the OTFT performance of the archetypical small-molecule p-type semiconductors P-BTDT (phenylbenzo[d,d]thieno[3,2-b;4,5-b]dithiophene) and pentacene using combined in situ sum frequency generation spectroscopy, atomic force microscopy, and grazing incidence and reflectance X-ray scattering. The molecular order and orientation of the OTFT components at the dielectric/semiconductor interface is probed as a function of SAM growth mode in order to understand how this impacts the overlying semiconductor growth mode, packing, crystallinity, and carrier mobility, and hence, transistor performance. This understanding, using a new, humidity-specific growth procedure, leads to a reproducible, scalable process for highly ordered OTS SAMs, which in turn nucleates highly ordered p-type semiconductor film growth, and optimizes OTFT performance. Surprisingly, the combined data reveal that while SAM molecular order dramatically impacts semiconductor crystalline domain size and carrier mobility, it does not significantly influence the local orientation of the overlying organic semiconductor molecules.
In vivo Assessment of RAS-Dependent Maintenance of Tumor Angiogenesis by Real-time Magnetic Resonance Imaging (2005)

New blood vessel formation is a prominent feature of human cancers and tumor progression and is frequently accompanied by the acquisition of an angiogenic phenotype associated with a switch in the balance of proangiogenic and antiangiogenic molecules. This study was designed to investigate the role of activated H-RAS on the angiogenic phenotype of melanoma that arises in the inducible Tyr/Tet-RAS Ink4a/Arf(-/-) model using in vivo imaging with histopathologic correlation. We show that loss of RAS activity in fully established melanomas led to a reduction in tumor volume, which was preceded by impairment of vascular function as determined by in vivo magnetic resonance imaging. This correlated with activation of apoptosis in host-derived endothelial cells as well as in tumor cells. Thus, real-time in vivo imaging provided evidence that maintenance of tumor angiogenesis requires activated RAS in this model system, and that loss of vascular integrity upon inactivation of RAS is an active process rather than a consequence of loss of tumor cell viability.
Supplemental Figure 1 from BET Bromodomain Inhibitors Enhance Efficacy and Disrupt Resistance to AR Antagonists in the Treatment of Prostate Cancer (2023)

&lt;p&gt;Enzalutamide resistant VCaP prostate cancer cells.&lt;/p&gt;
Einleitung (2023)

Die Arbeit beschäftigt sich mit der Schnittstelle zwischen Straf- und Aufsichtsrecht. Am Beispiel des § 54a KWG wird empirisch untersucht, wie sich das Strafrecht in Bereiche der prinzipienorientierten (Finanz-)Aufsicht einfügt. Um am kognitiven Potential der Institute zu partizipieren, normiert der Gesetzgeber mit § 25c Abs. 4a KWG prozedurale Anforderungen an das Risikomanagement und überlässt die konkrete Art der Umsetzung dieser Vorgaben den Instituten. Zugleich hat der Gesetzgeber mit § 54a KWG einen Verstoß gegen diese Risikomanagementvorgaben strafbewehrt. Dies führt zwangsläufig zu Spannungsverhältnissen – insbesondere mit den Zielen des Aufsichtsrechts und der vom Gesetzgeber normierten institutsinternen Wissensgenerierung.
Anti-Inflammatory Effect of Acetone Extracts from Microalgae Chlorella sp. WZ13 on RAW264.7 Cells and TPA-induced Ear Edema in Mice (2022)

Microalgae extracts have a wide range of uses in the field of healthcare and nutrition. However, the use of microalgae extracts in anti-inflammatory properties and their mechanism of action have not yet been fully studied. Here, we show that extracts from Chlorella sp. WZ13 (CSE-WZ13) dose-dependently reduced nitrite production, inhibited the expression of inducible nitric oxide synthase (iNOS) protein, and decreased the production of the gene and inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Using high-content imaging analysis, it was found that CSE-WZ13 inhibited the translocation of nuclear factor kappa B (NF-κB) from the cytoplasm to the nucleus. CSE-WZ13 also exerted anti-inflammatory effects in an ear edema mouse model induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). CSE-WZ13 inhibited edema by 36.17% and 25.66% at a dose of 0.3 and 0.1 mg/ear, respectively. Histological analysis showed that topical application of CSE-WZ13 decreased TPA-induced inflammatory cell infiltration. Our results indicate that CSE-WZ13 may be a useful candidate for the purpose of decreasing inflammation.
Anemia at older age: etiologies, clinical implications, and management (2017)

Anemia is quite frequently diagnosed in older individuals and is a key indicator of various reactive and clonal conditions. Many underlying diseases, like myelodysplastic syndrome (MDS), develop preferentially in elderly individuals. The prevalence of anemia at older age is increasing, and this is mainly attributable to more frequently applied diagnostics and demographic changes in our societies. The etiology of anemia at older age is complex and ranges from bone marrow failure syndromes to chronic kidney disease, and from nutritional deficiencies to inflammatory processes including inflammaging in immunosenescence. In a smaller number of cases, no clear-cut etiology is identified. These patients are referred to as unexplained anemia or idiopathic cytopenia of unknown significance. In others, somatic mutations in leukocytes are found, but diagnostic criteria for MDS or other hematologic diseases are not fulfilled, a condition termed clonal cytopenia of undetermined significance. Management of anemias at older age depends on (1) the severity of the anemia, (2) underlying condition(s), and (3) patient-related factors, including comorbidities. Even a mild anemia may substantially affect physical and cognitive capacities and quality of life. An underestimated aspect is that because of age-related changes, organ function such as erythropoietin production in the kidney may become suboptimal. Management and treatment of anemia in older patients often require a multidisciplinary approach and detailed investigations of organ function. In this article, we review current concepts around anemias at older age, with special emphasis on etiologies, clinical implications, and innovative concepts in the management of these patients.
Wrong to be Right (2015)

Most PSM are iatrogenic, with right-sided more likely to progress (and sooner) than left sided. Margin laterality is a heretofore unrecognized independent predictor of biochemical relapse and hints at the need to modify the traditional unilateral surgical technique.
Application of a multiprobe RNase protection assay and junctional sequences to define V beta gene diversity in Sezary syndrome. (1992)

Nineteen patients with mycosis fungoides/Sezary syndrome (MF/SZ), a malignancy of the mature helper T-cell phenotype (CD4+TCR alpha beta+), were screened for clonotypic V beta expansions in peripheral blood with a multiprobe RNase protection assay. A different predominant V beta gene was identified in 9 of 14 patients with high peripheral blood CD4/CD8 ratios, whereas 4 of these patients showed T-cell expansions expressing V beta genes other than those included in the assay. In contrast, five patients with few, if any, malignant cells in the circulation had V beta expression levels similar to that in normal peripheral blood. A unique V-D-J sequence was found for each highly expressed V beta gene, thereby documenting monoclonality of the expanded T-cell populations. Polymerase chain reaction (PCR) primers specific for the D-J beta junction accurately identified the corresponding malignant clonotype in peripheral blood. The diverse TCR V beta gene usage found in these MF/SZ patients suggests that T-cell receptor (TCR) specificity has no bearing on this disease.
Delayed-Release Suspending Aid Provides Cement-Slurry Stability in High-Temperature, Horizontal Wells (2015)

Abstract Successful liner cementing in unconventional shale wells is strongly dependent on slurry stability. A delayed-release, high-temperature suspending agent was developed that provides viscosification and stabilization of the slurry without causing excessive viscosification and mixing problems at the wellsite. The suspending aid was prepared from water-soluble, thermally stable monomers copolymerized with degradable crosslinking monomers. The crosslinks degrade as the temperature of the slurry increases, ultimately resulting in dissolution of the polymer and concomitant slurry viscosification. The performance of the suspending aid was demonstrated by means of laboratory testing under typical Eagle Ford shale conditions. Improvements were observed in terms of fluid-loss control (54 cc/30 min [control] to 28 cc/30 min), free fluid (5% [control] to 0%), sedimentation (Δρ 5.2 lbm/gal [control] to Δρ 0.2 lbm/gal), and consistometer off/on tests. Three field examples from the Eagle Ford are presented where the suspending aid was used to establish the desired mud-spacer-cement rheological hierarchy at bottomhole circulating temperature (BHCT); provide sufficient slurry stability to set the liner top plug, circulate out excess cement, and produce a competent cement sheath; and improve the mixability and stability of a barite-weighted spacer.
Microwave band demonstration of a reflective geometry fiber and free-space binary photonic delay line (1997)

For the first time, a modulated 2-b, one-channel binary switched, photonic time delay system (PTDS) is demonstrated that is based on a compact reflective optical delay path geometry that consists of one free-space delay line and one non-polarization-maintaining (PM) fiber delay line, Polarization switching using birefringent-mode nematic liquid crystals and a polarization noise-reduction technique are used to minimize the optical noise when using the cube polarization beamsplitters (PBS's) required in the reflective geometry delay, This gives the high electrical signal-to-noise ratios measured from 52 to 89 dB for the four settings of the delay line.
MIST Final Report: Multi-sensor Imaging Science and Technology (2008)

The Multi-sensor Imaging Science and Technology (MIST) program was undertaken to advance exploitation tools for Long Wavelength Infra Red (LWIR) hyper-spectral imaging (HSI) analysis as applied to the discovery and quantification of nuclear proliferation signatures. The program focused on mitigating LWIR image background clutter to ease the analyst burden and enable a) faster more accurate analysis of large volumes of high clutter data, b) greater detection sensitivity of nuclear proliferation signatures (primarily released gasses) , and c) quantify confidence estimates of the signature materials detected. To this end the program investigated fundamental limits and logical modifications of the more traditional statistical discovery and analysis tools applied to hyperspectral imaging and other disciplines, developed and tested new software incorporating advanced mathematical tools and physics based analysis, and demonstrated the strength and weaknesses of the new codes on relevant hyperspectral data sets from various campaigns. This final report describes the content of the program and the outlines the significant results.