gebhart/patrepeval huggingface dataset:
Protein Kinase C as a Modulator of 5HT1A and Dopamine-D2 Receptor Signaling (2003)

Since its identification as a family of protein kinases that is activated in the presence of calcium, phosphatidyl serine, and diacylglycerol (DAG), protein kinase C (PKC) has been regarded as one of the early steps in receptor signaling (Nishizuka, 1988, Nishizuka, 1995; Berridge, 1993; Rasmussen et al., 1995). The generation of the second messengers DAG and calcium by receptor-mediated phospholipase C (PLC) activation leads to rapid activation of several isoforms of PKC (Newton, 1995), its translocation to the membrane (Kiley et al., 1995), and the phosphorylation of numerous proteins. With the finding by Castagna et al. (1982) that phorbol esters (e.g., 12-O-tetrade-canoyl 4β-phorbol 13-acetate [TPA]) are potent and selective activators of multiple PKC isoforms, this pharmacological tool was used extensively to document the importance of PKC in a variety of stimulatory events: lymphocyte activation, contractile and secretory responses, gene transcription, and cell proliferation (Nishizuka, 1988,Nishizuka, 1995; Berridge, 1993; Rasmussen et al., 1995). In addition to these stimulatory actions, PKC activation was also found to have a negative feedback action to inhibit the activity of receptors that coupled to PLC to augment DAG and calcium levels.
[Recurrent anterior luxation of the shoulder occurring after the age of 40]. (1987)

Twenty-four recurrent dislocations of the shoulder with an onset after the age of 40 years have been operated on using the Trillat technique. The results have been studied with a mean follow-up of 10 years. This type of lesion, which is not rare, forming five per cent of cases in the authors series between 1953 and 1982, deserves separate consideration because of the frequency of associated rupture of the rotator cuff which seems to arise as a consequence of the first dislocation. There were 75 per cent of indirect tuberosity signs and 20 per cent of elevations of the humeral head before operation. It is responsible for osteoarthritis in the shoulder found in the follow-up. There were 70 per cent of osteoarthritic shoulders and 50 per cent of cases with elevation of the humeral head. The clinical results obtained, without any associated procedures on the cuff, were investigated by the criteria established for the surgery of shoulder instability (Rowe). They were satisfactory, with 63 per cent of good objective results and 88 per cent of satisfied patients. There appears to be a beneficial effect from the downward displacement of the subscapularis which should be taken into account as well as consideration of treatment for the lesions of the rotator cuff.
cis -Acting Sequences Required for Inducible Interleukin-2 Enhancer Function Bind a Novel Ets-Related Protein, Elf-1 (1992)

The recent definition of a consensus DNA binding sequence for the Ets family of transcription factors has allowed the identification of potential Ets binding sites in the promoters and enhancers of many inducible T-cell genes. In the studies described in this report, we have identified two potential Ets binding sites, EBS1 and EBS2, which are conserved in both the human and murine interleukin-2 enhancers. Within the human enhancer, these two sites are located within the previously defined DNase I footprints, NFAT-1 and NFIL-2B, respectively. Electrophoretic mobility shift and methylation interference analyses demonstrated that EBS1 and EBS2 are essential for the formation of the NFAT-1 and NFIL-2B nuclear protein complexes. Furthermore, in vitro mutagenesis experiments demonstrated that inducible interleukin-2 enhancer function requires the presence of either EBS1 or EBS2. Two well-characterized Ets family members, Ets-1 and Ets-2, are reciprocally expressed during T-cell activation. Surprisingly, however, neither of these proteins bound in vitro to EBS1 or EBS2. We therefore screened a T-cell cDNA library under low-stringency conditions with a probe from the DNA binding domain of Ets-1 and isolated a novel Ets family member, Elf-1. Elf-1 contains a DNA binding domain that is nearly identical to that of E74, the ecdysone-inducible Drosophila transcription factor required for metamorphosis (hence the name Elf-1, for E74-like factor 1). Elf-1 bound specifically to both EBS1 and EBS2 in electrophoretic mobility shift assays. It also bound to the purine-rich CD3R element from the human immunodeficiency virus type 2 long terminal repeat, which is required for inducible virus expression in response to signalling through the T-cell receptor. Taken together, these results demonstrate that multiple Ets family members with apparently distinct DNA binding specificities regulate differential gene expression in resting and activated T cells.
Severe Disease, Unaltered Leukocyte Migration, and Reduced IFN-γ Production in CXCR3−/− Mice with Experimental Autoimmune Encephalomyelitis (2006)

Experimental autoimmune encephalomyelitis (EAE) is a CD4(+) Th1 T cell-mediated disease of the CNS, used to study certain aspects of multiple sclerosis. CXCR3, the receptor for CXCL10, CXCL9, and CXCL11, is preferentially expressed on activated Th1 T cells and has been proposed to govern the migration of lymphocytes into the inflamed CNS during multiple sclerosis and EAE. Unexpectedly, CXCL10-deficient mice were susceptible to EAE, leaving uncertain what the role of CXCR3 and its ligands might play in this disease model. In this study, we report that CXCR3(-/-) mice exhibit exaggerated severity of EAE compared with wild-type (CXCR3(+/+)) littermate mice. Surprisingly, there were neither quantitative nor qualitative differences in CNS-infiltrating leukocytes between CXCR3(+/+) and CXCR3(-/-) mice with EAE. Despite these equivalent inflammatory infiltrates, CNS tissues from CXCR3(-/-) mice with EAE showed worsened blood-brain barrier disruption and more von Willebrand factor-immunoreactive vessels within inflamed spinal cords, as compared with CXCR3(+/+) mice. Spinal cords of CXCR3(-/-) mice with EAE demonstrated decreased levels of IFN-gamma, associated with reduced inducible NO synthase immunoreactivity, and lymph node T cells from CXCR3(-/-) mice primed with MOG(35-55) secreted less IFN-gamma in Ag-driven recall responses than cells from CXCR3(+/+) animals. CXCR3(-/-) lymph node T cells also showed enhanced Ag-driven proliferation, which was reduced by addition of IFN-gamma. Taken with prior findings, our data show that CXCL10 is the most relevant ligand for CXCR3 in EAE. CXCR3 does not govern leukocyte trafficking in EAE but modulates T cell IFN-gamma production and downstream events that affect disease severity.
Contact Mode Guided Motion Planning for Dexterous Manipulation. (2021)

Within the field of robotic manipulation, a central goal is to replicate the human ability to manipulate any object in any situation using a sequence of manipulation primitives such as grasping, pushing, inserting, sliding, etc. Conceptually, each manipulation primitive restricts the object and robot to move on a lower-dimensional manifold defined by the primitive's dynamic equations of motion. Likewise, a manipulation sequence represents a dynamically feasible trajectory that traverses multiple manifolds. To manipulate any object in any situation, robotic systems must include the ability to automatically synthesize manipulation primitives (manifolds) and sequence those primitives into a coherent plan (find a path across the manifolds). This paper investigates a principled approach for solving dexterous manipulation planning. This approach is based on rapidly-exploring random trees which use contact modes to guide tree expansion along primitive manifolds. This paper extends this algorithm from 2D domains to 3D domains. We validated our algorithm on a large collection of simulated 3D manipulation tasks. These tasks required our algorithm to sequence between 6-42 manipulation primitives (i.e.\! distinct contact modes). We believe this work represents an important step towards robotic manipulation capabilities which generalize across objects and environments.
Measurement of J/ψ production cross-sections in pp collisions at $$ \sqrt{s} $$ = 5 TeV (2021)

A bstract The production cross-sections of J/ψ mesons in proton-proton collisions at a centre-of-mass energy of $$ \sqrt{s} $$ <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:msqrt> <mml:mi>s</mml:mi> </mml:msqrt> </mml:math> = 5 TeV are measured using a data sample corresponding to an integrated luminosity of 9 . 13 ± 0 . 18 pb − 1 , collected by the LHCb experiment. The cross-sections are measured differentially as a function of transverse momentum, p T , and rapidity, y , and separately for J/ψ mesons produced promptly and from beauty hadron decays (nonprompt). With the assumption of unpolarised J/ψ mesons, the production cross-sections integrated over the kinematic range 0 &lt; p T &lt; 20 GeV/ c and 2 . 0 &lt; y &lt; 4 . 5 are $$ {\displaystyle \begin{array}{c}{\sigma}_{\mathrm{prompt}}\ J/\psi =8.154\pm 0.010\pm 0.283\ \upmu \mathrm{b},\\ {}{\sigma}_{\mathrm{nonprompt}}\ J/\psi =0.820\pm 0.003\pm 0.034\ \upmu \mathrm{b},\end{array}} $$ <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:mtable> <mml:mtr> <mml:mtd> <mml:msub> <mml:mi>σ</mml:mi> <mml:mtext>prompt</mml:mtext> </mml:msub> <mml:mspace/> <mml:mi>J</mml:mi> <mml:mo>/</mml:mo> <mml:mi>ψ</mml:mi> <mml:mo>=</mml:mo> <mml:mn>8.154</mml:mn> <mml:mo>±</mml:mo> <mml:mn>0.010</mml:mn> <mml:mo>±</mml:mo> <mml:mn>0.283</mml:mn> <mml:mspace/> <mml:mi>μb</mml:mi> <mml:mo>,</mml:mo> </mml:mtd> </mml:mtr> <mml:mtr> <mml:mtd> <mml:msub> <mml:mi>σ</mml:mi> <mml:mtext>nonprompt</mml:mtext> </mml:msub> <mml:mspace/> <mml:mi>J</mml:mi> <mml:mo>/</mml:mo> <mml:mi>ψ</mml:mi> <mml:mo>=</mml:mo> <mml:mn>0.820</mml:mn> <mml:mo>±</mml:mo> <mml:mn>0.003</mml:mn> <mml:mo>±</mml:mo> <mml:mn>0.034</mml:mn> <mml:mspace/> <mml:mi>μb</mml:mi> <mml:mo>,</mml:mo> </mml:mtd> </mml:mtr> </mml:mtable> </mml:math> where the first uncertainties are statistical and the second systematic. These cross-sections are compared with those at $$ \sqrt{s} $$ <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:msqrt> <mml:mi>s</mml:mi> </mml:msqrt> </mml:math> = 8 TeV and 13 TeV, and are used to update the measurement of the nuclear modification factor in proton-lead collisions for J/ψ mesons at a centre-of-mass energy per nucleon pair of $$ \sqrt{s_{\mathrm{NN}}} $$ <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:msqrt> <mml:msub> <mml:mi>s</mml:mi> <mml:mi>NN</mml:mi> </mml:msub> </mml:msqrt> </mml:math> = 5 TeV. The results are compared with theoretical predictions.
Asynchronous Communication Fostering Social Interaction with CollaboraTV (2010)

With the advent of digital video recorders and video-on-demand services, the way in which we consume media is undergoing a fundamental change. People today are less likely to watch shows at the same time, let alone the same place. As a result, television viewing which was once a social activity has been reduced to a passive, isolated experience. CollaboraTV was designed to address this new mode of television viewing by directly supporting asynchronous communication. We demonstrated its ability to support this communal viewing experience through a lab study and a month-long field study. Our studies show that users understand and appreciate the utility of asynchronous interaction, are enthusiastic about CollaboraTV’s engaging social communication primitives and value implicit show recommendations from friends. Our results both provide a compelling demonstration of a social television system and raise new challenges for social television communication modalities.
The Bacterial Effector HopX1 Targets JAZ Transcriptional Repressors to Activate Jasmonate Signaling and Promote Infection in Arabidopsis (2014)

Pathogenicity of Pseudomonas syringae is dependent on a type III secretion system, which secretes a suite of virulence effector proteins into the host cytoplasm, and the production of a number of toxins such as coronatine (COR), which is a mimic of the plant hormone jasmonate-isoleuce (JA-Ile). Inside the plant cell, effectors target host molecules to subvert the host cell physiology and disrupt defenses. However, despite the fact that elucidating effector action is essential to understanding bacterial pathogenesis, the molecular function and host targets of the vast majority of effectors remain largely unknown. Here, we found that effector HopX1 from Pseudomonas syringae pv. tabaci (Pta) 11528, a strain that does not produce COR, interacts with and promotes the degradation of JAZ proteins, a key family of JA-repressors. We show that hopX1 encodes a cysteine protease, activity that is required for degradation of JAZs by HopX1. HopX1 associates with JAZ proteins through its central ZIM domain and degradation occurs in a COI1-independent manner. Moreover, ectopic expression of HopX1 in Arabidopsis induces the expression of JA-dependent genes, represses salicylic acid (SA)-induced markers, and complements the growth of a COR-deficient P. syringae pv. tomato (Pto) DC3000 strain during natural bacterial infections. Furthermore, HopX1 promoted susceptibility when delivered by the natural type III secretion system, to a similar extent as the addition of COR, and this effect was dependent on its catalytic activity. Altogether, our results indicate that JAZ proteins are direct targets of bacterial effectors to promote activation of JA-induced defenses and susceptibility in Arabidopsis. HopX1 illustrates a paradigm of an alternative evolutionary solution to COR with similar physiological outcome.
Electron drift mobility of oxadiazole derivatives doped in polycarbonate (1995)

Charge drift mobilities of five oxadiazole derivatives doped in polycarbonate (PC) were evaluated with the time-of-flight technique. It is demonstrated that oxadiazoles incline to having electron-transport characteristics. In particular, an oxadiazole with naphthyl substituent (BND) was found to possess high potential of electron transport; the electron drift mobility of 50 wt % BND doped PC was 2.2×10−5 cm2 V−1 s−1 at an electric field of 7.5×105 V cm−1 at room temperature. In addition, incorporating strong electron-releasing substituents into oxadiazoles was demonstrated to add hole transport characteristics to oxadiazoles.
Prevention of Cold-Preservation Injury of CulturedEndothelial Cells by Catecholamines and Related Compounds (2003)

The present study was conducted to dissect the underlying mechanisms by which catecholamines protect cells against preservation injury. To this end, we firstly defined the cellular and molecular differences between protected and nonprotected cells and secondly defined the mediators that were involved in cold-induced damage. Cold storage of untreated human umbilical vein endothelial cells (HUVECs) resulted in profound cellular damage as assessed by lactate dehydrogenase (LDH) release and by morphological changes, e.g. cell size alterations and loss of cytoskeletal organization. Treatment of HUVECs with catecholamines before cold storage prevented cellular damage in a dose- and time-dependent fashion. Similar results were obtained with carvedilol or its hydroxylated derivative BM91.0228. Protection was not receptor-mediated and did not require de novo protein synthesis. The onset of protection occurred relatively quickly and the duration was long lasting. Addition of superoxide dismutase (SOD) to untreated HUVECs during cold preservation also was protective. Oxidation of catecholamines completely abrogated the protective effect of these compounds on cold-induced damage. Both at 4 degrees and 37 degrees C, catecholamines reduced the amount of reactive oxygen species (ROS) produced by HUVECs. In conclusion we have demonstrated that catecholamines protect cells against preservation injury either by scavenging of ROS or by inhibition of ROS production.
Hidrogenación catalítica selectiva de acetileno en presencia de exceso de etileno (2018)

Hidrogenación catalítica selectiva de acetileno en presencia de exceso de etileno. La presente invención describe un procedimiento para la purificación de corrientes de etileno de interés industrial que puede comprender, al menos, las siguientes etapas: - Una primera etapa en la que la corriente de etileno con acetileno y el hidrógeno se ponen en contacto con el catalizador que contiene nanopartículas de óxido de hierro. - Una segunda etapa de calentamiento de la mezcla obtenida. - Una tercera etapa de recuperación del producto. [ES]
Hyaluronic acid drives mesenchymal stromal cell-derived extracellular matrix assembly by promoting fibronectin fibrillogenesis (2021)

Hyaluronic acid (HA)-based biomaterials have been demonstrated to promote wound healing and tissue regeneration, owing to the intrinsic and important role of HA in these processes. A deeper understanding of the biological functions of HA would enable better informed decisions on applications involving HA-based biomaterial design. HA and fibronectin are both major components of the provisional extracellular matrix (ECM) during wound healing and regeneration. Both biomacromolecules exhibit the same spatiotemporal distribution, with fibronectin possessing direct binding sites for HA. As HA is one of the first components present in the wound healing bed, we hypothesized that HA may be involved in the deposition, and subsequently fibrillogenesis, of fibronectin. This hypothesis was tested by exposing cultures of mesenchymal stromal cells (MSCs), which are thought to be involved in the early phase of wound healing, to high molecular weight HA (HMWHA). The results showed that treatment of human bone marrow derived MSCs (bmMSCs) with exogenous HMWHA increased fibronectin fibril formation during early ECM deposition. On the other hand, partial depletion of endogenous HA led to a drastic impairment of fibronectin fibril formation, despite detectable granular presence of fibronectin in the perinuclear region, comparable to observations made under the well-established ROCK inhibition-mediated impairment of fibronectin fibrillogenesis. These findings suggest the functional involvement of HA in effective fibronectin fibrillogenesis. The hypothesis was further supported by the co-alignment of fibronectin, HA and integrin α5 at sites of ongoing fibronectin fibrillogenesis, suggesting that HA might be directly involved in fibrillar adhesions. Given the essential function of fibronectin in ECM assembly and maturation, HA may play a major enabling role in initiating and propagating ECM deposition. Thus, HA, as a readily available biomaterial, presents practical advantages for <i>de novo</i> ECM-rich tissue formation in tissue engineering and regenerative medicine.
Yield and Fruit Quality of Sixteen Fragaria vesca Accessions Grown in Southern Florida (2018)

Fragaria vesca is a diploid strawberry species that produces gourmet, aromatic fruits with only limited commercial production because of its relative obscurity. Most F. vesca research focuses on genetics and fruit aroma, but yield and fruit quality data across diploid accessions are lacking. Sixteen F. vesca accessions were grown in replicated field plots in southern Florida to measure field performance and fruit quality over multiple harvests during a single growing season. Accessions ‘Reine des Vallees’, ‘Baron Solemacher’, ‘Fragolina di Bosco’, and ‘Reugen’ all had significantly higher yield (115–140 g/plot/week) and fruit number (117–139 fruit/plot/week) compared with ‘Bowlenzauber’, ‘Attila’, ‘Ali Baba’, and ‘Pineapple Crush’ (31–57 g/plot/week and 32–60 fruit/plot/week) during peak production. Total average yield ranged from 240 g (‘Pineapple Crush’) to 1194 g (‘Baron Solemacher’) per plot of 10 plants. Fruit number and fruit yield were highly correlated ( R 2 = 0.96) for all accessions, and there was no significant difference in fruit weight among accessions through the entire season. Total soluble solids ranged from 10.9 to 13.5 °Brix, and fructose, glucose, sucrose, and total sugars ranged from 15.3 to 22.1, 13.5 to 20.0, 0.1 to 2.7, and 29.7 to 42.5 mg/g, respectively, fresh weight. Acidity ranged from 1.00% to 1.18% citric acid and was not consistently significantly different among accessions over multiple harvests. Forty-two aroma compounds were putatively identified over three harvests for each accession and included mostly esters and ketones with a few alcohols, terpenes, and aldehydes. The majority of these compounds were similarly abundant over harvests and among accessions with a few exceptions, including methyl anthranilate. These results are the first in-depth study of yield and fruit quality for a large number of F. vesca accessions that could lead to increased cultivation of this species for local markets.
ChemInform Abstract: SYNTHESIS OF NITROGEN‐ANALOGOUS Δ8‐TETRAHYDROCANNABINOLS (1977)

Abstract Umsetzung der Resorcine (I) mit dem Menthadienol (II) liefert die Tetrahydrocannabinol‐ Derivate (III); neben (IIIa) bzw. (IIIb) werden auch die Isomeren (IVa) und (IVb) gebildet.
Treatment of Wilson's disease with zinc: X. Intestinal metallothionein induction. (1992)

Oral zinc therapy is effective in controlling copper balance in patients with Wilson's disease and blocks the intestinal absorption of copper, as demonstrated by uptake of copper 64 and copper balance measurements. In this study, 64Cu uptake measurements were concomitantly carried out with intestinal biopsies to investigate the relationship of reduced copper absorption to the levels of intestinal metallothionein in patients with Wilson's disease at different stages of zinc therapy. A pronounced increase in intestinal metallothionein levels and a sharp drop in 64Cu absorption were found 4 to 5 days after the initiation of zinc treatment. Conversely, metallothionein levels decreased and 64Cu uptake increased on the discontinuation of zinc therapy. The data indicate that 64Cu absorption varies as a function of intestinal metallothionein level. Intestinal metallothionein levels were found to correlate linearly with urinary zinc levels, which reflect body zinc status. These findings support our hypothesis that intestinal metallothionein induction mediates decreased copper absorption observed during zinc therapy. The suppressive effect of zinc on copper absorption appears to have a half-life of about 11 days.
Dynamic contrast-enhanced magnetic resonance imaging of the lung reveals important pathobiology in idiopathic pulmonary fibrosis (2021)

DCE-MRI detects changes in the microvasculature and extravascular extracellular space in IPF, thus providing <i>in vivo</i> regional functional information.