gebhart/patrepeval huggingface dataset:
Design of an Interstitial Microwave Applicator for 3D Printing Antennas in the Body (2019)

3D printing in the body is an emerging technology with a host of biomedical applications, including wireless telemetry. An EM heating applicator can be used to "print" structures in the body using a biocompatible polymer material. This paper evaluates an applicator that could be used to produce focused heating for this purpose. This applicator is a sleeved-slot antenna operating at 2450 MHz. Simulations in brain tissue showed the applicator was capable of heating 3.3°C in 3 s.
High capacity Na+/H+ exchange activity in mineralizing osteoblasts (2010)

Osteoblasts synthesize bone in polarized groups of cells sealed by tight junctions. Large amounts of acid are produced as bone mineral is precipitated. We addressed the mechanism by which cells manage this acid load by measuring intracellular pH (pHi) in non-transformed osteoblasts in response to weak acid or bicarbonate loading. Basal pHi in mineralizing osteoblasts was ∼ 7.3 and decreased by ∼ 1.4 units upon replacing extracellular Na(+) with N-methyl-D-glucamine. Loading with 40 mM acetic or propionic acids, in normal extracellular Na(+), caused only mild cytosolic acidification. In contrast, in Na(+) -free solutions, weak acids reduced pHi dramatically. After Na(+) reintroduction, pHi recovered rapidly, in keeping with Na(+) /H(+) exchanger (NHE) activity. Sodium-dependent pHi recovery from weak acid loading was inhibited by amiloride with the Ki consistent with NHEs. NHE1 and NHE6 were expressed strongly, and expression was upregulated highly, by mineralization, in human osteoblasts. Antibody labeling of mouse bone showed NHE1 on basolateral surfaces of all osteoblasts. NHE6 occurred on basolateral surfaces of osteoblasts mainly in areas of mineralization. Conversely, elevated HCO 3- alkalinized osteoblasts, and pH recovered in medium containing Cl(-), with or without Na(+), in keeping with Na(+) -independent Cl(-) /HCO 3- exchange. The exchanger AE2 also occurred on the basolateral surface of osteoblasts, consistent with Cl(-) /HCO 3- exchange for elimination of metabolic carbonate. Overexpression of NHE6 or knockdown of NHE1 in MG63 human osteosarcoma cells confirmed roles of NHE1 and NHE6 in maintaining pHi. We conclude that in mineralizing osteoblasts, slightly basic basal pHi is maintained, and external acid load is dissipated, by high-capacity Na(+) /H(+) exchange via NHE1 and NHE6.
Source localization in reverberant environments: modeling and statistical analysis (2003)

Room reverberation is typically the main obstacle for designing robust microphone-based source localization systems. The purpose of the paper is to analyze the achievable performance of acoustical source localization methods when room reverberation is present. To facilitate the analysis, we apply well known results from room acoustics to develop a simple but useful statistical model for the room transfer function. The properties of the statistical model are found to correlate well with results from real data measurements. The room transfer function model is further applied to analyze the statistical properties of some existing methods for source localization. In this respect we consider especially the asymptotic error variance and the probability of an anomalous estimate. A noteworthy outcome of the analysis is that the so-called PHAT time-delay estimator is shown to be optimal among a class of cross-correlation based time-delay estimators. To verify our results on the error variance and the outlier probability we apply the image method for simulation of the room transfer function.
Highly Efficient Bioconversion of trans-Resveratrol to δ-Viniferin Using Conditioned Medium of Grapevine Callus Suspension Cultures (2022)

<i>δ</i>-Viniferin is a resveratrol dimer that possesses potent antioxidant properties and has attracted attention as an ingredient for cosmetic and nutraceutical products. Enzymatic bioconversion and plant callus and cell suspension cultures can be used to produce stilbenes such as resveratrol and viniferin. Here, <i>δ</i>-viniferin was produced by bioconversion from <i>trans</i>-resveratrol using conditioned medium (CM) of grapevine (<i>Vitis labruscana</i>) callus suspension cultures. The CM converted <i>trans</i>-resveratrol to <i>δ</i>-viniferin immediately after addition of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). Peroxidase activity and bioconversion efficiency in CM increased with increasing culture time. Optimized <i>δ</i>-viniferin production conditions were determined regarding H<sub>2</sub>O<sub>2</sub> concentration, incubation time, temperature, and pH. Maximum bioconversion efficiency reached 64% under the optimized conditions (pH 6.0, 60 °C, 30 min incubation time, 6.8 mM H<sub>2</sub>O<sub>2</sub>). In addition, in vitro bioconversion of <i>trans</i>-resveratrol was investigated using CM of different callus suspension cultures, showing that addition of <i>trans</i>-resveratrol and H<sub>2</sub>O<sub>2</sub> to the CM led to production of <i>δ</i>-viniferin via extracellular peroxidase-mediated oxidative coupling of two molecules of <i>trans</i>-resveratrol. We thus propose a simple and low-cost method of <i>δ</i>-viniferin production from <i>trans</i>-resveratrol using CM of plant callus suspension cultures, which may constitute an alternative approach for in vitro bioconversion of valuable molecules.
The complete foxhunter / by Charles Richardson. (1908)

shrine of the hound ; and when any pack of hounds of great reputation is for sale, either privately or by auction, there is not only much excitement in the hunting world, but probably the sale is on so great a scale that it will be talked about for years to come.Just as this book goes to press a sale of thirty hounds for a sum of ^2000 has been announced, and the price of over ^66 per hound, though a high one, is not a record.It is the case, moreover, that there is a steady increase in the number of hunting people, in spite of the fact that it costs more to hunt than it did a generation or two ago.But probably the increase is greater with regard to beagles and other hounds than in fields composed of men and women who ride.Not so long ago hunting on foot was little indulged in, except in the mountainous districts of Cumberland and West- morland, and with a few packs of otter hounds.Now, however, there are whole communities of people who hunt on foot, scattered all over the country, and probably about a third of each field of foot hunters is composed of ladies.During the winter, too, very full reports are given each week of the doings of many of the principal packs of hounds, and there is every reason to believe that the supply of hunting news is by no means equal to the demand.In the following pages some attempt is made to treat of foxhunting as it is carried on at the present day, and to show the great changes which have come into existence--not in the actual sport itself, but in the conditions which surround it.Every one who followed As regards the science of hunting we shall be almost silent, being of opinion that the question is best treated of by men who have not only held long periods of office as masters of hounds, but who have carried the horn Huntings etc., by Colonel Cook, was published in 1826; but whereas " Beckford " is a household word xvi THE COMPLETE FOXHUNTER amongst hunting men, Colonel Cook is only appre- ciated by a few enthusiasts.Extracts from the Diary of a Huntsman, by Thos.Smith (1838), at one time master of the Cravenand actually master of the Pytchley when his book was published-has also much useful information on hounds and their work ; and this was followed, only a year later, by The Noble Science, by F. P. Delme Radcliffe, who for several seasons was master of the Hertfordshire.Then, after a lapse of eight years, came Notitia Venatica, by R
Security verification for authentication and key exchange protocols (2008)

In a ubiquitous environment, it is preferable for authentication and key exchange protocols to be optimized automatically in accordance with the security requirements. In this paper, we propose a security verification method for the authentication and key exchange protocols that is based on Bellare et al.' s model. In particular, we show the verification points of one security property for authentication protocols and five security properties for key exchange protocols. We show that this method is valid by verifying four typical examples of the authentication and key exchange protocols.
Annual review of biophysics and biophysical chemistry (1985)

This book contains articles reviewing significant developments in the fields of biophysics and biophysical chemistry, and related topics.
Thin-film filament-based solar cells and modules (1997)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation J. R. Tuttle, E. D. Cole, T. A. Berens, J. Alleman, J. Keane; Thin-film filament-based solar cells and modules. AIP Conf. Proc. 5 April 1997; 404 (1): 243–250. https://doi.org/10.1063/1.53474 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAIP Publishing PortfolioAIP Conference Proceedings Search Advanced Search |Citation Search
Early phase I results of recombinant immunotoxin HA22 in patients with B-cell leukemia (2008)

3039 Background: HA22 is a 63 kDa recombinant anti-CD22 immunotoxin containing the variable domains of the anti-CD22 Mab RFB4 connected to truncated Pseudomonas exotoxin. HA22 is a mutant form of BL22, which induced CR in 61% of patients with CdA-pretreated hairy-cell leukemia (HCL), and partial or marginal response (PR or MR) in chronic lymphocytic leukemia (CLL). BL22 is much more cytotoxic for HCL compared to CLL ex vivo; the median IC50 is 4.3 ng/ml in 33 patients with HCL vs 430 ng/ml in 70 patients with CLL (p < 0.0001). Compared to BL22, HA22 contains THW replacing SSY in the CDR3 domain of the variable heavy domain, which improves binding affinity to CD22 by 15-fold. CLL cells from 17 patients are a median of 8-fold more sensitive to HA22 than to BL22. Methods: Patients with HCL and CLL were treated on 2 separate multicenter phase I protocols. Eligibility required at least 2 prior standard therapies and a medical indication for treatment including either disease-related cytopenias, symptoms or increasing tumor burden. Patients were treated with HA22 by 30 min infusion every other day for 3 doses (QOD x3), with cycles repeated every 28 days. Dose-escalation in each trial required 0 of 3 or 1 of 6 patients with dose limiting toxicity (DLT) by cycle 2 day 10, or by cycle 1 day 28 if patients were followed without DLT and not retreated. Neutralizing antibodies in the serum and active HA22 in the plasma were quantified by cytotoxicity assays using CD22+ Raji cells. Results: Nine HCL and 11 CLL patients received HA22 at 5, 10, or 20 ug/Kg QOD x3, with no DLT. High levels of neutralizing antibodies were observed in 1 (14%) of 7 HCL and 0 of 7 CLL patients evaluated. In 13 of 14 evaluable patients, peak plasma levels were higher with the 3rd than with the 1st dose (median 1.8-fold, range 0.9–11-fold, p < 0.0001), either due to rapidly decreasing tumor burden or clearing of soluble CD22. The median half-life with the 3rd dose of HA22 was 66 min (range 23–121 min). Despite low dose levels, patients with HCL responded with 1 (20%) CR and 4 (80%) PR in 5 evaluable patients. Patients with CLL have had decreases in circulating CLL cells. Conclusions: HA22 at early dose levels is active in HCL and without significant toxicity in both HCL and CLL. There is no evidence of increased toxicity due to the increased binding of HA22 to CD22. Author Disclosure Employment or Leadership Consultant or Advisory Role Stock Ownership Honoraria Research Expert Testimony Other Remuneration Medimmune
Star‐Shaped Poly(styrene)‐block‐Poly(4‐vinyl‐N‐methylpyridiniumiodide) for Semipermanent Antimicrobial Coatings (2013)

Goal of the present work is to develop an antimicrobial coating that can be applied from an aqueous solution and resists short washing cycles, but can be rinsed off by thorough washing. To this end, a series of star-shaped polystyrene-block-poly(4-vinyl-N-methylpyridinium iodide) polymers are synthesized by anionic polymerization using a core-first approach. The optimal resulting polymers are applied as coatings on glass slides, showing high antimicrobial efficiency against Staphylococcus aureus as well as Escherichia coli. The coatings, characterized by atomic force microscopy and transmission electron microscopy, stay at the surface even after at least 20 flush-like washings with water, and retain their antimicrobial activity.
Konzentrationsmessung von Feststoffkomponenten in Rauchgasen auf Basis der Raman-Spektroskopie (2005)

Die trockene Rauchgasentschwefelung, bei der Schwefeldioxid mittels disperser Kalkpartikel in einer Gas/Feststoff-Reaktion in einem Reaktionsrohr zu Gips umgesetzt wird, besitzt ein großes Potenzial zur Betriebsoptimierung. Voraussetzung dafür ist die detaillierte Kenntnis des Umsetzungsgerades, insbesondere bei sich ständig ändernden Betriebsparametern. Die vorgestellten Ergebnisse sind Vorversuche einer auf der Raman-Spektroskopie basierenden Messmethode, die als quasi Online-Verfahren die bisher üblichen diskontinuierlichen Probennahmen und zeitversetzten Laboranalysen ablösen soll.
PERCEPTION OF PHYSICAL ACTIVITIES BEFORE AND AFTER A 16 WEEK WEIGHT LOSS PROGRAM (2001)

The Surgeon General and several public health organizations advocate the accumulation of 30 minutes of moderate intensity physical activity (PA) per day. These recommendations are especially important for obese patients who do not enjoy vigorous exercise. However, little is known about obese individuals' perceptions of what constitutes appropriate PA and what “Counts” as exercise. Therefore, the purpose of this study was to investigate obese adults' perceptions of PAs varying in duration and intensity before and after a 16-week weight loss program. Thirty-three obese adults (24 women; 9 men) were recruited for a 16 week weight loss program with the intervention of exercise and caloric restriction (women: 1200–1500kcal/d; men: 1500–1800kcal/d). Participants were given a questionnaire with a visual analog scale before and after weight loss to investigate the perception of “how much certain PAs count as exercise?” The analog scale ranged from “1: not counting as exercise” to “9: counting very much as exercise.” Before weight loss, typical lifestyle PAs such as a two minute walk (2.7 ± 2.1; Mean ± SD), five minute walk (3.6 ± 2.1), gardening (4.3 ± 2.4), parking far from a mall entrance (4.4 ± 2.3) and playing hopscotch were found to be perceived as counting least as exercise. Activities perceived as counting most as exercise were weight training (7.9 ± 1.2), rope jumping (8.3 ± 0.8), aerobics class (8.4 ± 0.9), jogging (8.5 ± 0.8) and cross country skiing (8.6 ± 0.7). All traditional exercises rated were regarded as counting significantly more than the typical lifestyle PAs (p < .001). Similarly, after weight loss, the activities rated as counting the least and most remained the same. Even though recommendations suggest that moderate intensity activities may provide health benefits, these results suggest that PA that is of lesser intensity and duration may not be perceived as counting towards exercise when compared to more traditional modes of PA. These data suggest that exercise scientists may need to make obese patients aware that lifestyle PA is a option to manage their weight and improve their health. Supported by NIH grant M400-215-2111 and the GCRC at Johns Hopkins Bayview Medical Center
Structural Characteristics of Oligomeric DNA Strands Adsorbed onto Single-Walled Carbon Nanotubes (2012)

The single-stranded DNA to single-walled carbon nanotube (SWCNT) hybrid continues to attract significant interest as an exemplary biological molecule-nanomaterial conjugate. In addition to their many biomedical uses, such as in vivo sensing and delivery of molecular cargo, DNA-SWCNT hybrids enable the sorting of SWCNTs according to their chirality. Current experimental methods have fallen short of identifying the actual structural ensemble of DNA adsorbed onto SWCNTs that enables and controls several of these phenomena. Molecular dynamics (MD) simulation has been a useful tool for studying the structure of these hybrid molecules. In recent studies, using replica exchange MD (REMD) simulation we have shown that novel secondary structures emerge and that these structures are DNA-sequence and SWCNT-type dependent. Here, we use REMD to investigate in detail the structural characteristics of two DNA-SWCNT recognition pairs: (TAT)(4)-(6,5)-SWCNT, i.e., DNA sequence TATTATTATTAT bound to the (6,5) chirality SWCNT, and (CCG)(2)CC-(8,7)-SWCNT as well as off-recognition pairs (TAT)(4)-(8,7)-SWCNT and (CCG)(2)CC-(6,5)-SWCNT. From a structural clustering analysis, dominant equilibrium structures are identified and show a right-handed self-stitched motif for (TAT)(4)-(6,5) in contrast to a left-handed β-barrel for (CCG)(2)CC-(8,7). Additionally, characteristics such as DNA end-to-end distance, solvent accessible SWCNT surface area, DNA hydrogen bonding between bases, and DNA dihedral distributions have been probed in detail as a function of the number of DNA strands adsorbed onto the nanotube. We find that the DNA structures adsorbed onto a nanotube are also stabilized by significant numbers of non-Watson-Crick hydrogen bonds (intrastrand and interstrand) in addition to π-π stacking between DNA bases and nanotube surface and Watson-Crick pairs. Finally, we provide a summary of DNA structures observed for various DNA-SWCNT hybrids as a preliminary set of motifs that may be involved in the functional role of these hybrids.
Accelerating FPGA debug (2014)

FPGA technology is commonly used to prototype new digital designs before entering fabrication. Whilst these physical prototypes can operate many orders of magnitude faster than through a logic simulator, a fundamental limitation is their lack of on-chip visibility when debugging. To counter this, trace-buffer-based instrumentation can be installed into the prototype, allowing designers to capture a predetermined window of signal data during live operation for offline analysis. However, instead of requiring the designer to recompile their entire circuit every time the window is modified, this article proposes that an overlay network is constructed using only spare FPGA routing multiplexers to connect all circuit signals through to the trace instruments. Thus, during debugging, designers would only need to reconfigure this network instead of finding a new place-and-route solution. Furthermore, we describe how this network can deliver signals to both the trigger and trace units of these instruments, which are implemented simultaneously using dual-port RAMs. Our results show that new network configurations connecting any subset of signals to 80--90% of the available RAM capacity can be computed in less than 70 seconds, for a 100,000 LUT circuit, as many times as necessary. Our tool—QuickTrace—is available for download.
336-OR: Mandarin Fish Ranavirus Viral Insulin/IGF-Like Peptide Inhibits Human IGF-1 Receptor (2021)

Our recent studies published in PNAS and Molecular Metabolism showed for the very first time that viruses possess genes encoding viral insulin/IGF-1 like peptides (VILPs). These two studies provided several important insights on VILPs: 1. Identified VILPs in six different Iridoviridae viruses 2. Showed that chemically synthesized single chain (sc, IGF-1 like) and double chain (dc, insulin-like) VILPs can bind to human insulin and IGF-1 receptors and stimulate downstream signaling. 3. Demonstrated that VILPs can increase glucose uptake in vitro and in vivo. 4. Identified the unique tissue-specific effects of VILPs. 5. Found sequences of these viruses in the human gut and plasma virome. In the present study, we focused on a novel, Mandarin Fish Ranavirus (MFRV) VILP and characterized both sc and dc forms for the first time. MFRV scVILP binds to human IGF1R with an affinity 100x higher than insulin, making it a VILP with the highest affinity to this receptor so far discovered. MFRV scVILP also activates IGF1R phosphorylation and post-receptor signaling. Interestingly, although MFRV scVILP is a weak agonist of IGF1R, it inhibits IGF-1 signaling at higher concentrations. When we treat cells with both IGF-1 and MFRV VILP, MFRV scVILP inhibits IGF-1 (10nM) stimulated IGF1R phosphorylation and activation of PI3K and MAPK pathways at high concentrations (&amp;gt; 250 nM). Surprisingly, the dc version of MFRV VILP neither bind nor stimulate IGF1R. This suggests that the C-chain of MFRV VILP is essential for both binding to IGF1R and the IGF-1 inhibitory effects. Taken together, our results revealed that MFRV scVILP is a novel antagonist/inhibitor of IGF-1R. IGF1R signaling is overly activated in breast, prostate and colorectal cancers. Despite tremendous efforts to develop an effective inhibitor of IGF1R, these attempts failed. MFRV VILP has the potential to contribute development of new therapeutics in cancer therapy and to help us better understand ligand-receptor interactions and mechanisms of IGF1R inhibition. Disclosure M. Chrudinova: None. V. Gelfanov: Employee; Self; Novo Nordisk, Employee; Spouse/Partner; Eli Lilly and Company. L. Zakova: None. J. Cutone: None. A. Powis: None. K. Sevgi: None. J. Jiracek: None. R. Dimarchi: Employee; Self; Novo Nordisk. E. Altindis: None. Funding National Institute of Diabetes and Digestive and Kidney Diseases (K01DK117967)
Synthesis of sulfur-doped porous carbon from heavy coker gas oil and its application in CO2 capture (2020)

Elemental sulfur is used to cross-link heavy coker gas oil (HKGO) with only 0.44 wt% olefinic hydrogen content in the absence of solvent; subsequent pyrolysis yields sulfur-doped porous carbon with Brunauer–Emmett–Tell (BET) surface area (s.a.) around 1714–1785 m<sup>2</sup> g<sup>−1</sup>.
Automatic instance generation using simulation for inductive learning (1994)

Inductive learning can be used to extract rules required for an expert system which assists in output analysis for system simulation. However, several examples of the system, constituting an instance set, are required for learning to take place. Generating the required instance set to be used by-an inductive learning algorithm is time consuming and complex. This paper is an attempt to clarify this problem, discuss its complexity and suggest context related solutions. A procedure for automatic instance generation is then proposed. The proposed procedure is a combination of three search methods (grid based, forward search ,backward search).