gebhart/patrepeval huggingface dataset:
Clinical characteristics and treatment outcome of disease-related peripheral neuropathy in Waldenstrom's macroglobulinemia (WM). (2010)

8114 Background: Peripheral neuropathy (PN) is a debilitating morbidity in WM, whose natural history and treatment outcome is largely unknown. Methods: We examined the incidence, characteristics, and treatment outcome of disease related PN in 900 WM patients. Results: 199 (22.1%) patients had disease related PN with a higher incidence in sporadic vs. familial WM (23.5% vs. 15.6%; p=0.02). PN patients had lower serum IgM (p=0.02), B2M (p=0.02), and BM disease burden (p=0.001). Among 122 PN patients evaluated for neuropathic antibodies, 24.5%, 1.64%, and 0.81% were positive for MAG, GM1, and sulfatide antibodies, respectively. Patients with neuropathic antibodies had lower serum IgM (p=0.0002) and BM disease burden (p=0.001) vs. non-PN patients. 13/61 (21.3%) patients examined for amyloid were positive, and only 1 had a neuropathic antibody. The median time to treatment for all PN patients was 9 months. Among non-amyloid related PN patients who received PP or IVIG as their first intervention, 29/42 (69%) and 1/8 (12.5%) reported symptomatic improvement, respectively. 151 PN patients received frontline CTX which consisted of an oral alkylator, nucleoside analogue or rituximab; or rituximab combination with a nucleoside analogue, cyclophosphamide, thalidomide or bortezomib. Of these, 71 (47%) and 8 (5.3%) had improvement or complete resolution of PN following CTX, respectively. Symptomatic improvement was more likely with non-amyloid related PN (48.5% vs. 15.4%; p=0.045); in patients who achieved a major response, i.e. ≥50% reduction in serum IgM (79% vs. 35.5%; p<0.0001); those who received earlier therapy, i.e. ≤24 months (57.3% vs. 42.5%; p=0.06); and those who received rituximab combination vs. any monotherapy (59.3% vs. 37.0%; p=0.007; p=0.06 vs. rituximab alone). Conclusions: PN is a common morbidity, particularly among sporadic WM patients. PP for non-amyloid related PN is an effective interim intervention. Improvements in symptomatic neuropathy occur in half of PN patients following CTX, and are more likely to occur in WM patients with non- amyloid related PN, those who receive earlier therapy, combination therapy with rituximab, and who achieve a major response. Author Disclosure Employment or Leadership Position Consultant or Advisory Role Stock Ownership Honoraria Research Funding Expert Testimony Other Remuneration Biogen Idec, Celgene, Genentech, Millennium Biogen Idec, Celgene, Genentech, Millennium Celgene, Millennium
Structure and Expression of Chloroplast-Localized Porphobilinogen Deaminase from Pea (Pisum sativum L.) Isolated by Redundant Polymerase Chain Reaction (1993)

Porphobilinogen (PBG) deaminase catalyzes the polymerization of four PBG monopyrrole units into the linear tetrapyrrole hydroxymethylbilane necessary for the formation of chlorophyll and heme in plant cells. Degenerate oligonucleotide primers were designed based on amino acid sequence data (generated by mass spectrometry) for purified PBG deaminase from pea (Pisum sativum L.) chloroplasts. These primers were used in TaqI polymerase-catalyzed polymerase chain reaction (PCR) amplification to produce partial cDNA and nuclear genomic fragments encoding the enzyme. Subsequently, a 1.6-kb cDNA was isolated by screening a cDNA library constructed in lambda gt11 from leaf poly(A)+ RNA with the PCR products. The cDNA encodes an approximately 40-kD polypeptide containing a 46-amino acid NH2-terminal transit peptide and a mature protein of 323 amino acids. The deduced amino acid sequence of the mature pea enzyme is similar to PBG deaminases from other species and contains the conserved arginine and cysteine residues previously implicated in catalysis. Northern blot analysis indicates that the pea gene encoding PBG deaminase is expressed to varying levels in chlorophyll-containing tissues and is subject to light induction.
Are Libraries Worth Investing in?: Finnish University Libraries and their Effect on the National Economy (2009)

The paper discusses the correlation between the growth of the gross national product (GNP) of Finland and the library materials costs of the academic libraries during thirty years (1977–2006). Statistical data were used in the analysis and it was found that there is some correlation between the trends of these two. The paper discusses different possibilities for interpretation of this outcome, but the authors draw the conclusion that it is clearly worth investing in libraries as one tool to improve the knowledge economy.
Fabrication of high‐mobility n ‐type carbon nanotube thin‐film transistors on plastic film (2013)

Abstract We fabricated flexible n ‐type carbon nanotube thin‐film transistors on a plastic film by a simple transfer process and a solution‐based chemical doping technique with polyethyleneimine (PEI). There exists an optimal PEI concentration, which is determined by doping level and charge accumulation in the PEI layer. A high electron mobility of 70 cm 2 /Vs was achieved with an on/off ratio of 10 5 in ambient air. (© 2013 WILEY‐VCH Verlag GmbH &amp; Co. KGaA, Weinheim)
T Lymphocytes Transduced with a Lentiviral Vector Expressing F12-vif Are Protected from HIV-1 Infection in an APOBEC3G-Independent Manner (2005)

The viral infectivity factor (Vif) is an essential component of the HIV-1 infectious cycle. Vif counteracts the action of the cytidine deaminase APOBEC3G (AP3G), which confers nonimmune antiviral defense against HIV-1 to T lymphocytes. Disabling or interfering with the function of Vif could represent an alternative therapeutic approach to AIDS. We have expressed a natural mutant of Vif, F12-Vif, in a VSV-G-pseudotyped lentiviral vector under the Tat-inducible control of the HIV-1 LTR. Conditional expression of F12-Vif prevents replication and spreading of both CXCR4 and CCR5 strains of HIV-1 in human primary T lymphocyte and T cell lines. T cells transduced with F12-Vif release few HIV-1 virions and with reduced infectivity. Several lines of evidence indicate that HIV-1 interference requires the presence of both wild-type and F12-Vif proteins, suggesting a dominant-negative feature of the F12-Vif mutant. Surprisingly, however, the F12-Vif-mediated inhibition does not depend on the reestablishment of the AP3G function.
Buying bestsellers online: A case study in Search & Searchability. (2002)

A website’s design directly affects how well search engines can crawl, match and rank its pages. For this reason, searchability is an important concern in site design. We study the interaction between search engines and Web sites by means of a case study of online bookstores and general-purpose search engines. The task modelled is that of finding web pages from which a book, described by its title, may be purchased. We first compared the relative effectiveness of search engines in finding pages matching the criterion, regardless of bookstore. Then we compared the relative searchability of the bookstore websites by observing how many times each bookstore contributed useful answers to the search results. Large differences in the performance of both search engines and bookstores were observed. Two of the search engines performed better than their peers, and one bookstore was far more searchable than all others. To further explore these differences we tabulate the total number of pages from each bookshop which are included in the search engine indexes. We conclude with recommendations both to bookstores on how they may improve their Web presence, and to search engines on how they may improve their performance for product searches.
Capillary electrophoresis as a simple and sensitive method to study polysaccharides of Sinorhizobium sp. NGR234 (2003)

We report the minimum amounts of polysaccharides (6.5 ng) that can be studied using capillary electrophoresis-laser-induced fluorescence (CE-LIF) and describe the separation of the various monosaccharides susceptible to occur in Sinorhizobium cell wall. On-gel hydrolysis of the polysaccharides is described. It allowed the easy and rapid determination of the monosaccharide composition of the excised lipopolysaccharides. The composition obtained fits with gas chromatography-mass spectrometry (GC-MS) results. This study underlines the simplicity of using CE-LIF, which remains an entirely "water medium" method, unlike GC-MS which is, in addition, less sensitive (at least 100-fold) than CE-LIF.
Relapsed Hodgkin Lymphoma in Older Patients: A Comprehensive Analysis From the German Hodgkin Study Group (2013)

OS in older patients with RR-HL can be predicted using a simple prognostic score. Poor outcome in high-risk patients cannot be overcome by any of the applied treatment strategies. Our results might help to guide treatment decisions and evaluate new compounds in these patients.
P.26: Constant vs. Non‐constant Luminance Video Signals for UHDTV (2013)

Abstract The luminance and chrominance video‐signal format is beneficially applied for editing broadcasting programs in a studio and for delivering those from broadcasters to end users. Recently, a new ITU‐R recommendation has been published for UHDTV baseband image format. Two types of video signal were proposed: constant vs. non‐constant luminance video signals. This study introduces in depth analysis results for these two different signals in the aspect of crosstalk characteristics and the preservation of the original luminance information. And the compression‐efficiency evaluation results are also given.
The role of microRNAs in the regulation of cancer stem cells (2014)

Cancer stem cells (CSCs) have been reported in many human tumors and are proposed to drive tumor initiation and progression. CSCs share a variety of biological properties with normal somatic stem cells such as the capacity for self-renewal, the propagation of differentiated progeny, and the expression of specific cell surface markers and stem cell genes. However, CSCs differ from normal stem cells in their chemoresistance and tumorigenic and metastatic activities. Despite their potential clinical importance, the regulation of CSCs at the molecular level is not well-understood. MicroRNAs (miRNAs) are a class of endogenous non-coding RNAs that play an important role in the regulation of several cellular, physiological, and developmental processes. Aberrant miRNA expression is associated with many human diseases including cancer. miRNAs have been implicated in the regulation of CSC properties; therefore, a better understanding of the modulation of CSC gene expression by miRNAs could aid the identification of promising biomarkers and therapeutic targets. In the present review, we summarize the major findings on the regulation of CSCs by miRNAs and discuss recent advances that have improved our understanding of the regulation of CSCs by miRNA networks and may lead to the development of miRNA therapeutics specifically targeting CSCs.
Nitrogen‐Doped Mesoporous Carbon Electrodes Prepared from Templating Propylamine‐Functionalized Silica (2020)

Abstract In this paper, nitrogen doped carbon materials with mesopores 4–8 nm‐wide and a high surface area of ca .1100 m 2 g −1 were synthesized according to an innovative hard template method. Sucrose was used as carbon source and propylamine functionalized silica acted as both nitrogen source and templating agent. The novel doped carbons were compared with mesoporous carbons featuring similar texture properties (pore size and surface area) but obtained by the pyrolysis of sucrose or 1,10‐phenantholine and employing non‐functionalized silica as a templating agent. The interest of this investigation is to understand how doping occurs when a functionalized silica is employed, and whether the nitrogen doping remains a surface property or it is extended also to the bulk of the material, influencing the morphological and the electrical properties of the resulting carbon. X‐ray photoemission spectroscopy, elemental analysis, and EDX confirmed the doping action of the functionalized silica and the electrochemical characterization allowed to compare the different performances as supercapacitor materials.
G protein-coupled estrogen receptor 1-mediated effects in the rat myometrium (2011)

G protein-coupled estrogen receptor 1 (GPER), also named GPR30, has been previously identified in the female reproductive system. In this study, GPER expression was found in the female rat myometrium by reverse transcriptase-polymerase chain reaction and immunocytochemistry. Using GPER-selective ligands, we assessed the effects of the GPER activation on resting membrane potential and cytosolic Ca(2+) concentration ([Ca(2+)](i)) in rat myometrial cells, as well as on contractility of rat uterine strips. G-1, a specific GPER agonist, induced a concentration-dependent depolarization and increase in [Ca(2+)](i) in myometrial cells. The depolarization was abolished in Na(+)-free saline. G-1-induced [Ca(2+)](i) increase was markedly decreased by nifedipine, a L-type Ca(2+) channel blocker, by Ca(2+)-free or Na(+)-free saline. Intracellular administration of G-1 produced a faster and transitory increase in [Ca(2+)](i), with a higher amplitude than that induced by extracellular application, supporting an intracellular localization of the functional GPER in myometrial cells. Depletion of internal Ca(2+) stores with thapsigargin produced a robust store-activated Ca(2+) entry; the Ca(2+) response to G-1 was similar to the constitutive Ca(2+) entry and did not seem to involve store-operated Ca(2+) entry. In rat uterine strips, administration of G-1 increased the frequency and amplitude of contractions and the area under the contractility curve. The effects of G-1 on membrane potential, [Ca(2+)](i), and uterine contractility were prevented by pretreatment with G-15, a GPER antagonist, further supporting the involvement of GPER in these responses. Taken together, our results indicate that GPER is expressed and functional in rat myometrium. GPER activation produces depolarization, elevates [Ca(2+)](i) and increases contractility in myometrial cells.
Magnetic method for measuring moisture content using diamagnetic characteristics of water (2016)

Moisture content measurements of rice kernels and soil are important for agriculture. Therefore, in this study, a new measurement method using the diamagnetic characteristics of water was developed for measurements of the moisture content of rice kernels and soil. The magnetic characteristics of the samples were determined using a magnetometer developed by us based on a superconducting quantum interference device. Because of the diamagnetic characteristics of water, the susceptibility of rice kernels became more negative with increasing moisture content. In the case of soil, which is a mixture of diamagnetic and ferromagnetic materials, a second-harmonic detection method using AC with DC bias magnetic field was applied to reduce the influence of the ferromagnetic signal. The intensity of the second-harmonic signal of a soil was determined to be proportional to its moisture content.
Identification of Amino Acids Required for the Functional Up-regulation of Human Dihydrofolate Reductase Protein in Response to Antifolate Treatment (2005)

Human dihydrofolate reductase (DHFR) protein levels rapidly increase upon exposure to methotrexate, a potent inhibitor of this enzyme. A model to explain this increase proposes that DHFR inhibits its own translation by binding to its cognate mRNA and that methotrexate disrupts the DHFR protein-mRNA complex allowing its translation to resume. In the present study, Chinese hamster ovary cells lacking DHFR were transfected with wild type and mutants of human DHFR to identify amino acids that are essential for increases in DHFR in response to methotrexate. Glu-30, Leu-22, and Ser-118 were involved in the up-regulation of DHFR protein levels by methotrexate and certain other antifolates. Cells transfected with E30A, L22R, and S118A mutants that did not respond to methotrexate up-regulation had higher basal levels of DHFR, consistent with the model, i.e. lack of feedback regulation of these enzymes. Although cells containing the S118A mutant enzyme had higher levels of DHFR and had catalytic activity similar to that of wild type DHFR, they had the same sensitivity to the cytotoxicity of methotrexate, as were cells with wild type DHFR. This finding provides evidence that the adaptive up-regulation of DHFR by methotrexate contributes to the decreased sensitivity to this drug. Based on these observations, a new model is proposed whereby DHFR exists in two conformations, one bound to DHFR mRNA and the other bound to NADPH. The mutants that are not up-regulated by methotrexate are unable to bind their cognate mRNA.
Effects of charge collectors in ferroelectric E-field sensors (2012)

A nonlinear dynamical system based on ferroelectric capacitors coupled into a unidirectional ring circuit, which under particular conditions exhibits an oscillating regime of behavior, is considered with particular interest for developing novel electric field sensors. For such a device a weak, external, target electric field interacts with the system thus inducing perturbation of the polarization of the ferroelectric material; the target signal can be indirectly detected and quantified via its effect on the system response. The conceived devices exploit the synergetic use of bi-stable ferroelectric materials, micromachining technologies that allow us to address charge density amplification, and implement novel sensing strategies based on coupling non-linear elemental cells. The “charge collector” strategy, already discussed in [1], has been employed to detect the target field. An experimental characterization of the whole circuit, including three cells coupled in a ring configuration, has been carried out. The effect of the dimensions of the charge collectors has also been investigated.
Hypothermic regulation of astrocyte proteome profile in experimental stroke (2012)

Astrocytes provide physical and metabolic support for neurons in the central nervous system. Reactive astrocytes, however, play a crucial role in neuroinflammation after brain injury. Therapeutic hypothermia has been shown to be neuroprotective in brain injury patients. In order to understand the effect of therapeutic hypothermia on astrocytes in stroke patients, we performed a proteomic profiling of astrocytes following transient focal cerebral ischemia in rats under normothermic (37°C) or mild hypothermic condition (33°C). Primary astrocytes were prepared from rat brain using a Percoll gradient method, and their proteome profiles were determined by using LC/ESI-MS/MS followed by high-throughput label-free quantification. Comparison of proteome profiles of astrocytes following transient focal ischemia revealed that hypothermia upregulated (≥1.5-fold) 86 proteins and downregulated (≤0.6-fold) 47 proteins compared to normothermic condition. The changes of protein expression were validated by Western blot or RT-PCR. Ingenuity Pathways Analysis and Database for Annotation, Visualization and Integrated Discovery analyses of the up- or downregulated proteins indicate that hypothermia influences glutamatergic signaling, cell death, and stress response of astrocytes in the ischemic brain. Therefore, therapeutic hypothermia may be neuroprotective in stroke patients by modulating astrocytic functions and survival.
Isolation and Biological Activity of New Norhirsutanes from Creolophus cirrhatus (2008)

Five new norhirsutanes, named creolophins A-E, and complicatic acid were isolated from the culture broth of the rare tooth fungus Creolophus cirrhatus by solvent extraction, silica gel column chromatography and HPLC. In addition, neocreolophin, a complex dimerization product, was formed as an artefact during purification. The structures were elucidated by spectroscopic methods and are published in a separate paper. Two of the metabolites showed moderate antibacterial, antifungal and cytotoxic activities.
Autonomous deployment of mobile sensors network in an unknown indoor environment with obstacles (2018)

We developed a Voronoi-based algorithm, called Bio-Inspired Self Organizing Network (BISON), designed to provide a successful deployment of wireless sensor network (WSN) following fast, cost-efficient and self-organizing process, autonomously adapting to the unknown topology of the target environment, and avoiding obstacles discovered in real-time. To limit the power consumed during the deployment, BISON restricts each node to use only locally sensed information to adapt to live-discovered topology while avoiding obstacles and connecting with neighboring nodes. The algorithm is evaluated with respect to several metrics, and simulation results showed faster convergence to a fully connected network with lower deployment costs compared to similar algorithms reported in the literature.
Polynuclear and halogenated structures in polyphenylenes synthesized from benzene, biphenyl, and p‐terphenyl under various conditions: Characterization by laser desorption/fourier transform mass spectrometry (1986)

Abstract Polyphenylenes made by reaction of benzene, biphenyl, or p ‐terphenyl with metal halide catalyst–oxidant systems are complex mixtures of dissimilar oligomers, which include halogenated and polynuclear structures, according to positive and negative‐ion laser desorption/Fourier transform mass‐spectral analyses. Polymerization of benzene with metal‐chloride salts that terminate chain elongation by chlorination of the end rings appears to decrease formation of polynuclear structures by providing a competing pathway for chain termination. Polynuclear structures occur to a greater extent with oligomerization of biphenyl than with benzene, presumably because of isomerization and increased opportunity for π overlap during propagation. Electrical conductivities of polyphenylenes made by various routes should not be discussed solely in terms of the linear poly( p ‐phenylene) structure.