gebhart/patrepeval huggingface dataset:
Shape-morphing composites with designed micro-architectures (2016)

Shape memory polymers (SMPs) are attractive materials due to their unique mechanical properties, including high deformation capacity and shape recovery. SMPs are easier to process, lightweight, and inexpensive compared to their metallic counterparts, shape memory alloys. However, SMPs are limited to relatively small form factors due to their low recovery stresses. Lightweight, micro-architected composite SMPs may overcome these size limitations and offer the ability to combine functional properties (e.g., electrical conductivity) with shape memory behavior. Fabrication of 3D SMP thermoset structures via traditional manufacturing methods is challenging, especially for designs that are composed of multiple materials within porous microarchitectures designed for specific shape change strategies, e.g. sequential shape recovery. We report thermoset SMP composite inks containing some materials from renewable resources that can be 3D printed into complex, multi-material architectures that exhibit programmable shape changes with temperature and time. Through addition of fiber-based fillers, we demonstrate printing of electrically conductive SMPs where multiple shape states may induce functional changes in a device and that shape changes can be actuated via heating of printed composites. The ability of SMPs to recover their original shapes will be advantageous for a broad range of applications, including medical, aerospace, and robotic devices.
Bandpass–Bandstop Filter Cascade Performance Over Wide Frequency Tuning Ranges (2010)

A tunable, substrate integrated, high Q bandpass-bandstop filter cascade is demonstrated that is capable of providing up to 100 dB of isolation between two dynamically selectable frequencies of interest. Evanescent-mode cavity filters are used in the cascade to allow high quality factors and wide tuning ranges. It is shown that it is theoretically possible to tune the cascade circuit's passband and transmission nulls independently over more than an octave in frequency while displaying good performance. General coupling matrix theory, ABCD matrix theory, and measurements are shown that describe the behavior of the filter cascade over the entire frequency tuning range. The high, dynamic isolation provided by cascade circuits has potential to be useful in concurrent transmit-receive systems, shared aperture systems, and spectral environments with strong co-site interference.
GOLGA8 increases bulk antisense oligonucleotide uptake and activity in mammalian cells (2023)

Antisense oligonucleotides (ASOs) are short synthetic nucleic acids that recognize and bind to complementary RNA to modulate gene expression. It is well established that single-stranded, phosphorothioate-modified ASOs enter cells independent of carrier molecules, primarily via endocytic pathways, but that only a small portion of internalized ASO is released into the cytosol and/or nucleus, rendering the majority of ASO inaccessible to the targeted RNA. Identifying pathways that can increase the available ASO pool is valuable as a research tool and therapeutically. Here, we conducted a functional genomic screen for ASO activity by engineering GFP splice reporter cells and applying genome-wide CRISPR gene activation. The screen can identify factors that enhance ASO splice modulation activity. Characterization of hit genes uncovered GOLGA8, a largely uncharacterized protein, as a novel positive regulator enhancing ASO activity by ∼2-fold. Bulk ASO uptake is 2- to 5-fold higher in GOLGA8-overexpressing cells where GOLGA8 and ASOs are observed in the same intracellular compartments. We find GOLGA8 is highly localized to the <i>trans</i>-Golgi and readily detectable at the plasma membrane. Interestingly, overexpression of GOLGA8 increased activity for both splice modulation and RNase H1-dependent ASOs. Taken together, these results support a novel role for GOLGA8 in productive ASO uptake.
Drug-Induced Trafficking of P-Glycoprotein in Human Brain Capillary Endothelial Cells as Demonstrated by Exposure to Mitomycin C (2014)

P-glycoprotein (Pgp; ABCB1/MDR1) is a major efflux transporter at the blood-brain barrier (BBB), restricting the penetration of various compounds. In other tissues, trafficking of Pgp from subcellular stores to the cell surface has been demonstrated and may constitute a rapid way of the cell to respond to toxic compounds by functional membrane insertion of the transporter. It is not known whether drug-induced Pgp trafficking also occurs in brain capillary endothelial cells that form the BBB. In this study, trafficking of Pgp was investigated in human brain capillary endothelial cells (hCMEC/D3) that were stably transfected with a doxycycline-inducible MDR1-EGFP fusion plasmid. In the presence of doxycycline, these cells exhibited a 15-fold increase in Pgp-EGFP fusion protein expression, which was associated with an increased efflux of the Pgp substrate rhodamine 123 (Rho123). The chemotherapeutic agent mitomycin C (MMC) was used to study drug-induced trafficking of Pgp. Confocal fluorescence microscopy of single hCMEC/D3-MDR1-EGFP cells revealed that Pgp redistribution from intracellular pools to the cell surface occurred within 2 h of MMC exposure. Pgp-EGFP exhibited a punctuate pattern at the cell surface compatible with concentrated regions of the fusion protein in membrane microdomains, i.e., lipid rafts, which was confirmed by Western blot analysis of biotinylated cell surface proteins in Lubrol-resistant membranes. MMC exposure also increased the functionality of Pgp as assessed in three functional assays with Pgp substrates (Rho123, eFluxx-ID Gold, calcein-AM). However, this increase occurred with some delay after the increased Pgp expression and coincided with the release of Pgp from the Lubrol-resistant membrane complexes. Disrupting rafts by depleting the membrane of cholesterol increased the functionality of Pgp. Our data present the first direct evidence of drug-induced Pgp trafficking at the human BBB and indicate that Pgp has to be released from lipid rafts to gain its full functionality.
O-Benzyl-N-tert-butyloxycarbonyl-L-serine (1970)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTO-Benzyl-N-tert-butyloxycarbonyl-L-serineVictor J. Hruby and Kenneth W. EhlerCite this: J. Org. Chem. 1970, 35, 5, 1690Publication Date (Print):May 1, 1970Publication History Published online1 May 2002Published inissue 1 May 1970https://pubs.acs.org/doi/10.1021/jo00830a100https://doi.org/10.1021/jo00830a100research-articleACS PublicationsRequest reuse permissionsArticle Views276Altmetric-Citations18LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Improved performance of In(Ga)As/GaAs quantum dot solar cells via light scattering by nanoparticles (2009)

InAs quantum dots have been used to extend the absorption edge of InGaAs/GaAs quantum well solar cells from 940 to ∼1100 nm. In order to improve absorption of infrared radiation by the thin (300 nm) active layer, we exploit its high refractive index, which acts as a waveguide for certain frequencies of light. Surface-deposited nanoparticles scatter incident radiation into waveguide modes of the devices, yielding improved infrared photocurrent generation of at least 10% at all wavelengths between 700 and 1100 nm, short-circuit current density increases of up to 16%, and corresponding gains in power conversion efficiency.
High endothelial cells synthesize and degrade sLex. Putative implications for L‐selectin‐dependent recognition (1999)

L-selectin guides lymphocytes into peripheral lymphoid tissues by recognizing glycoprotein ligands decorated with 6-sulfated sialyl Lewis x (sulfo sLex). Here we have used a rat peripheral lymph node high endothelial cell line (Ax) to study in detail the synthesis, expression and degradation of sLex epitope. We show here that Ax cells possess active alpha(1,3)fucosyltransferase Fuc-TVII, the enzyme responsible for the final fucosylation of sialyl-N-acetyllactosamine during sLex synthesis, and express sLex on the cell surface. Furthermore, these cells degrade sLex, primarily by desialylating it to neutral Lex epitopes by alpha(2,3)sialidase(s).
Simple methods for estimating the performance and specification of optical components with anisotropic mid-spatial frequency surface errors (2019)

Specification and tolerancing of surfaces with mid-spatial frequency (MSF) errors are challenging and require new tools to augment simple surface statistics to better represent the structured characteristics of these errors. A novel surface specification method is developed by considering the structured and anisotropic nature of MSF errors and their impact on the modulation transfer function (MTF). The result is an intuitive plot of bandlimited RMS error values in polar coordinates which contains the surface error anisotropy information and enables an easy to understand acceptance criterion. Methods, application examples, and the connection of this surface specification approach to the MTF are discussed.
Media Content Management (2002)

The production of multimedia content has grown overwhelmingly in recent years due to decreasing hardware costs, inexpensive storage and ubiquitous high-bandwidth networks. In addition, the introduction of digital video is completely changing the landscape of the entire video value chain. The easy access and increased availability of multimedia is posing new challenges to manage the data. Image and video archives in broadcast studios, corporate archives of multimedia collaborative sessions, video conferencing sessions and educational videos all require tools for quickly locating video segments of desired content with transparent access. The market for such tools, known as media asset-management tools, has been growing rapidly. In this chapter we will discuss the general architecture and the tools for media content management. We will survey some of the techniques that employ methods of data management beyond traditional databases. We will discuss existing research prototypes and some commercially available systems. These systems enable applications that facilitate effective access, interaction, browsing and display of complex and inhomogeneous information consisting of images, video and audio.
Towards polarisation independent silicon optical modulators for high speed data transmission (2011)

We report a 40 Gb/s optical modulation with similar performance for TE and TM polarisation using a depletion diode inserted in both arms of a Mach Zhender interometer.
Flow Resistance of Surface Micromachined Microfluidic Channels (2004)

In this paper measured pressure vs. flow and flow resistance are compared with theoretical predictions (Poiseuille flow) for surface micromachined microfluidic channels with thin-film deformable covers. Three sets of data are compared. In data set 3 the channel width is narrow, channel deflections are relatively small, and there is very little deviation from the theoretical flow resistance prediction. In data sets 1 and 2 there is significant deviation from Poiseuille flow predictions—with flow resistances as little as 1/2 the predicted values that decrease with increasing pressure. Two hypotheses to explain this discrepancy are discussed: (1) channel cover deflection leading to deeper, lower resistance flow channels, and (2) an observed two-phase, air/water, flow phenomena leading to reduced effective wall friction in the channel.
ChemInform Abstract: Synthesis of pseudo‐Ribofuranoses by Stereocontrolled Reactions on 4‐ Hydroxycyclopent‐2‐enylmethanol Derivatives. (1993)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Supplementary Figure 5 from PI3K Inhibition Impairs BRCA1/2 Expression and Sensitizes BRCA-Proficient Triple-Negative Breast Cancer to PARP Inhibition (2023)

&lt;p&gt;PDF file - 162K, Western blot of cell lysates from MDA-MB-468 or PDC44 cells treated with 750nM and 500nM BKM120 respectively for 4 days using the indicated antibodies&lt;/p&gt;
Synthesis of water-soluble sugar derivatives of combretastatin A-4 (1995)

Three sugar derivatives (6, 7 and 13) of the antimitotic agent combretastatin A-4 1 have been synthesized. Two of these water-soluble derivatives (6, 13) showed strong cytotoxicity to the murine P388 cell line.
A dual-modality system for imaging anatomy and vasculature in live mouse embryos (2012)

An imaging system that combined high-frequency ultrasound (HFU) with photoacoustics (PA) for in vivo visualization of anatomical and vascular maps in transgenic mouse embryos is presented. The system consisted of a five-element, 40-MHz, poly-vinylidenefluoride-co-trifluoroethylene (PVDF-TrFE) annular array with a hole in the center to facilitate coaxial delivery of light produced by a 532-nm pulsed laser. A phosphate buffer-filled petri-dish with a central hole was placed on the abdomen of an anesthetized mouse and a laparotomy was performed to expose an intact uterus. The probe was raster scanned to achieve 3-D imaging. The central element of the array was excited with a high-voltage impulse synchronized with the light pulse. The resulting ultrasound echo and PA signal from each scan location were digitized from all five array channels and synthetically focused in post-processing. The embryonic vasculature depicted by the PA image was co-registered with anatomical features represented in the HFU image. The head and the abdomen of embryos were imaged; feasibility of real-time, spatially co-registered, dual-modality in vivo imaging of mouse embryos was demonstrated.
Activity of single-chain antibodies that selectively block the expression of Bcl-2 and Bag-1 anti-apoptotic proteins. (1997)

Background: The Bcl-2 gene becomes dysregulated in a wide variety of human cancers, including the majority of low grade Non-Hodgkin lymphoma, some B-cell chronic lymphocytic leukemias and acute myelogenous leukemias. The Bcl-2 protein blocks programmed cell death(apoptosis). In addition, high level of Bcl-2 expression contribute to tumor radio- and chemoresistance and is associated with poor clinical prognosis in some lymphoma patients. The recently described Bag-1 protein is another anti-apoptotic protein which has been found to interact physically and functionally with Bcl-2, although it's role in malignancies has not yet been defined. Methods: Based on these concepts, a targeted anti-cancer strategy was designed to selectively eradicate Bcl-2 and Bag-1 overexpressing tumor cells. To this end, we have employed intracellular immunization using single-chain antibodies (sFv). We have constructed specific sFvs against the Bcl-2 and Bag-1 proteins. The variable light (VL) and heavy(VH) regions of those murine monoclonal antibodies were derived, using specific primers, from cDNA generated from the hybridoma cell lines. The VL and VH chains were linked together by overlapping PCR. The sFvs were expressed into E. Coli and the soluble fraction of the protein was collected. Results: Expression of the sFvs was verified by Western blot (WB) and their binding affinity was assessed by ELISA. The anti-Bcl-2/Bag-1 sFvs that showed good binding to their target were subcloned into an ER-targeted mammalian expression vector. Expression of these sFvs into mammalian cells was shown by WB. For initial functional analysis, HeLa cells were cotransfected with the sFv and its corresponding target. In this transient assay, the anti-Bag-1 sFvs with specific binding activity against Bag-1 blocked the expression of the protein in HeLa cells. Similar results were obtained with the anti-Bcl-2 sFvs. Conclusions: The anti-Bag-1/Bcl-2 sFvs represent valuable agents for the potential elimination of tumor cells overexpressing these proteins in a bone marrow purging context. We are currently screening a variety of malignant cell lines for Bag-1 and Bcl-2 overexpression. Based on these preliminary findings, introduction of our sFvs in tumor cells expressing high level of Bag-1 and/or Bcl-2 should increase their sensitivity to radiation or chemotherapy-induced apoptosis.
Heartbeat Measurement Results by a VHF-Band Antenna with an Adaptive Matching Control Algorithm (2021)

Non-contact detection using a Doppler sensor has been studied for heartbeat measurement. However, Doppler sensors are affected by reflected waves from surrounding objects, making accurate acquisition of heartbeats difficult. To solve this problem, we have proposed the heartbeat sensor using of the small loop antenna with low radiation efficiency. When it is placed near subject's heart, the antenna input impedance change according to surroundings. In this paper, we show an adaptive control algorithm of a variable matching circuit according to changes in the surroundings of the antenna. The effectiveness of the proposed method is verified by measurements for multiple persons.
Strategies for Identification of HIV-1 Integrase Inhibitors (2006)

HIV-1 integrase, which catalyzes the joining of viral DNA to the host cell DNA, has attracted considerable attention as a target for the design and screening of novel anti-HIV drugs as it is essential for virus replication and the establishment of persistent infection. Progress in the identification of different classes of compounds that block integrase activity has been summarized recently in several excellent reviews. Here, we present a brief overview of integrase inhibition, highlighting some of the unusual properties of this protein and important considerations in searching for potential new inhibitors and their evaluation.
3D eWLB (embedded wafer level BGA) technology for 3D-packaging/3D-SiP (Systems-in-Package) applications (2009)

Demand for wafer level packaging (WLP) is being driven by the need to shrink package size and height, simplify the supply chain and provide a lower overall cost by using the infrastructure of a batch process. There are some restrictions in possible applications for Fan-In WLPs since global chip trends tend toward smaller chip areas with an increasing number of interconnects. The shrinkage of the pitches and pads at the chip to package interface is happening much faster than the shrinkage at the package to board interface. This interconnection gap requires fan-out packaging, where the package size is larger than the chip size in order to provide a sufficient area to accommodate the 2nd level interconnects. eWLB (embedded Wafer Level BGA) is a type of fan-out WLP that has the potential to realize any number of interconnects with standard pitches at any shrink stage of the wafer node technology. Furthermore, 3D eWLB technology enables 3D IC and 3D SiP packaging with vertical interconnection. 3D eWLB can be implemented with through silicon via (TSV) applications as well as discrete component embedding. In this paper, there will be discussion of the recent advancements in 3D eWLB packaging and integration as well as what is being envisioned and developed to address future technology requirements in 3D packaging and 3D SIP. The advantage of 3D eWLB technology and applications of 3D packaging will be presented with several examples. The process flow of 3D eWLB fabrication, assembly and packaging challenges, and performance characteristics will be also discussed.
Monitoring trapped charge generation for gate oxide under stress [MOS capacitors] (1997)

A measurement method to extract the respective quantities and centroids of positive and negative trapped charges, i.e., Q/sub p/ and Q/sub n/, generated by the negative current stress for gate oxides is proposed and demonstrated. The method is based on neutralization of and by a low positive current stress to differentiate the effects of Q/sub p/ and Q/sub n/. From the extracted quantities and centroids of Q/sub p/ and Q/sub n/ of negatively stressed oxides, it was found that Q/sub p/ and Q/sub n/ are generated near the oxide/substrate interface and Q/sub p/ is initially much larger than Q/sub n/. After the continuous stressing, Q/sub p/ saturates and moves closer to the interface, but Q/sub n/ keeps increasing and moves away from the interface, especially for those oxides after the post-poly anneal (PPA) treatment. Q/sub p/ is very unstable and easily neutralized, either by a small stress of opposite polarity or the same polarity. For the latter, Q/sub p/ is mainly dependent on the level of the final stressing field.
Mass spectrometric analysis ofKlebsiella pneumoniae ssp.pneumoniae rough strain R20 (O1− : K20−) lipopolysaccharide preparations: identification of novel core oligosaccharide components and three 3-deoxy-D-manno-oct-2-ulopyranosonic artifacts (1999)

In an attempt to find the best approach for the mass spectrometric analysis of the whole range of lipopolysaccharide (LPS) structures from Klebsiella pneumoniae ssp. pneumoniae rough strain R20 (O1-:K20-), various methods of LPS preparation were applied and the products were analyzed using a range of mass spectrometric techniques. The most productive approach proved to be the removal of lipid A by mild acid hydrolysis and the study of the core oligosaccharide structures using nanoelectrospray time-of-flight mass spectrometry (TOF-MS) in combination with collision-induced dissociation tandem mass spectrometry. This procedure is very sensitive, but results in the generation of a reducing 3-deoxy-D-manno-oct-2-ulopyranosonic acid residue (Kdo) that is susceptible to the formation of artifacts, which give rise to pseudomolecular ions 18, 46, and 88 Da below the pseudomolecular ion for the unmodified species. Alternatively, matrix-assisted laser desorption/ionization TOF-MS combined with post-source decay can be used to study the de-O-acylated LPS preparation and especially to identify those residues bearing phosphate groups and the residues involved in the linkage between the core and lipid A. In addition to the five LPS core structures defined using NMR spectroscopy by Süsskind et al., several extra related LPS structure were identified. Larger LPS species were observed, which surprisingly do not represent species containing longer versions of the novel Klebsiella heptoglycan, but instead are species having the defined core and heptoglycan extended with up to three extra hexuronic acid and one or two extra hexose residues.
1 - PATIENTS (PTS) WITH RECURRENT GYNECOLOGIC CANCER WHOSE TUMORS HAVE ACTIVATING WNT PATHWAY MUTATIONS RESPOND BETTER TO DKN-01, A DICKKOPF-1 (DKK1) INHIBITOR (2019)

Objectives: Wnt/β-catenin signaling is frequently dysregulated in gynecologic malignancies. CTNNB1, APC and RNF43 mutations cause pathway activation; CTNNB1 stabilizing mutations lead to elevated DKK1 expression which promotes an immune suppressive tumor microenvironment. Neutralization by DKN-01 (D), a mAb against DKK1, is being tested in a phase 2 basket study. Methods: