gebhart/patrepeval huggingface dataset:
The simian virus 40 minimal origin and the 72-base-pair repeat are required simultaneously for efficient induction of late gene expression with large tumor antigen. (1984)

We have studied the temporal regulation of simian virus 40 (SV40) late gene expression by construction and transient expression analysis of plasmids containing the transposon Tn9 chloramphenicol acetyltransferase gene placed downstream from the late control region. The SV40 origin region in the early (but not the late) orientation promotes chloramphenicol acetyltransferase gene expression efficiently in monkey cells lacking large tumor (T) antigen. In monkey cells producing T antigen, the promoter activity of the late control region is induced by approximately 1,000-fold above the basal level. By deletion and point mutagenesis, we define two domains of the late control region required for efficient induction with T antigen. Domain I is the minimal replication origin containing T-antigen binding site II. Domain II consists of the 72-base-pair (bp) repeat and a 19-bp downstream sequence up to nucleotide 270. Domains I and II should act synergistically because the absence of either one or the other decreases induction efficiency by 2 orders of magnitude. Though a complete copy of domain II is optimal, the origin-proximal 22-bp portion of this domain is sufficient. The 21-bp repeat, located between domains I and II, is dispensable for this induction, as are sequences located downstream from nucleotide 270 in the late orientation.
IFN Unresponsiveness in LNCaP Cells Due to the Lack of JAK1 Gene Expression (2005)

We reported previously that 23% of human lung adenocarcinoma cell lines were unresponsive to IFN-gamma. To extend this finding to cancer cells derived from distinct tissues of origin, we assessed IFN-gamma receptor signaling in the LNCaP human prostate adenocarcinoma cell line, which in previous experiments by others failed to induce a range of IFN-dependent biological responses. In this report, we show that LNCaP cells fail to respond to either IFN-gamma or IFN-alpha because of an impairment in the proximal signaling events downstream of both IFN-gamma and IFN-alpha/beta receptors that lead to the activation of STAT1. Furthermore, we show that LNCaP insensitivity to the IFNs is a result of the absence of expression of the JAK1 kinase, an obligate component shared by both IFN-gamma and IFN-alpha/beta receptors. JAK1 was undetectable in LNCaP cells at both protein and message levels. Treatment of LNCaP cells with a combination of inhibitors of DNA methyltransferases and histone deacetylases induced expression of JAK1 message. These results identify the molecular basis for IFN insensitivity in the LNCaP cell line and suggest that epigenetic silencing of key immunologic signaling components may be one mechanism by which tumor cells evade immune detection and elimination.
Performance of In/sub 0.53/Ga/sub 0.47/As and InP junction field-effect transistors for optoelectronic integrated circuits. I. Device analysis (1989)

The authors have developed an analytical model to study In/sub 0.53/Ga/sub 0.47/As and InP junction field-effect transistors (JFETs) for use in InP-based optoelectronic integrated circuits (OEICs). This model includes the effects of channel resistance and band-to-band tunneling. The agreement between the calculations and experimental results supports the validity of the model. The authors discuss the optimum design for these JFETs and compare their performance quantitatively. In order to prevent device performance from being degraded by the effects of tunneling, the optimum channel dopings of In/sub 0.53/Ga/sub 0.47/As and InP JFETs are found to be 7*10/sup 16/ and 5*10/sup 17/ cm/sup -3/, respectively. In/sub 0.53/Ga/sub 0.47/As JFETs not operated in the tunneling regime show at least a 40% higher efficiency than InP JFETs in terms of the power dissipated per transconductance. The authors conclude that In/sub 0.53/Ga/sub 0.47/As JFETs are well suited for very-high-density monolithic integration, where power efficiency must be high.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Model-based dependency analysis in service delivery process management (2010)

Build up well-defined and optimized service process is the key to deliver good service quality and service satisfaction. An effective method of dependency analysis in the service delivery process is the core to construct an optimized process. However, with increasingly complicated services, there is the large amount of the tasks elements with extreme complex dependency in the service process. Under this situation, set up correct relationship among tasks becomes time consuming and error prone. In this paper, we propose a model-based dependency analysis to automatically build up the dependency relationship among tasks in the service delivery process. It addresses the problem in analyzing dependency firstly and our approach is given. Based on the dependency analysis, we also propose several advanced analysis features on the process to guide user to optimize the process via reducing the process cost. Also a tool adopting our approach has been implemented and introduced. Based on the tool, a case study about the test service delivery process is illustrated to show the results.
Molecular biology and pharmacology of cloned opioid receptors 1 (1995)

The cloning and expression of DNA for the three major opioid receptor types (mu, delta, and kappa) present new research opportunities for the characterization of opioid drugs and their interactions with these receptors. Genomic and cDNA clones for opioid receptors exist for several animal species including mouse, rat, guinea pig, and human. These include clones for all three human opioid receptor types. The receptor proteins consist of about 400 amino acids and have the characteristic seven transmembrane domain structure of G-protein-coupled receptors. There is about 60% amino acid identity between opioid receptor types and about 90% identity between a receptor type cloned from different animal species. All opioid receptor types mediate the inhibition of adenylyl cyclase in response to agonist binding. Radioligand binding and functional studies using the cloned receptors tend to support current conclusions on opioid drug receptor selectivity and activity. Investigations of opioid receptor chimeras and single amino acid mutants are providing information on the ligand recognition sites of these receptors and essential support for the development of computational opioid receptor models. A molecular model of the human delta opioid receptor is included in this review.
Lagrangian Data Analysis in Mesoscale Prediction and Model Validation Studies (1999)

Abstract : The long term goals of this proposal are the development and application of new methods of investigation for the use of Lagrangian data. Special attention is given to the combined use of data and models, in terms of model validation and statistical prediction.
Correction to Correlating Nanorod Structure with Experimentally Measured and Theoretically Predicted Surface Plasmon Resonance (2011)

ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionNEXTORIGINAL ARTICLEThis notice is a correctionCorrection to Correlating Nanorod Structure with Experimentally Measured and Theoretically Predicted Surface Plasmon ResonanceAbrin L. Schmucker, Nadine Harris, Matthew J. Banholzer, Martin G. Blaber, Kyle D. Osberg, George C. Schatz*, and Chad A. Mirkin*Cite this: ACS Nano 2011, 5, 9, 7685Publication Date (Web):August 17, 2011Publication History Received28 July 2011Published online17 August 2011Published inissue 27 September 2011https://pubs.acs.org/doi/10.1021/nn2028659https://doi.org/10.1021/nn2028659correctionACS PublicationsCopyright © 2011 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views912Altmetric-Citations3LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (860 KB) Get e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Approximation,Chemical calculations,Chemical structure,Quantum mechanics Get e-Alerts
Nonlinear Phase Shifts Using Second Order Nonlinearities (1992)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text M. Sheik-Bahae, R. DeSalvo, D. J. Hagan, G. Assanto, G. Stegeman, and E. W. Van Stryland, "Nonlinear Phase Shifts Using Second Order Nonlinearities," Optics & Photonics News 3(12), 11-11 (1992) Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
Fourier transform infrared imaging as a method for detection of HLA class I expression in melanoma without the use of antibody (2007)

Human leukocyte antigen (HLA) class I expression in melanoma is usually assessed using immunohistochemical staining. Here we report on the use of Fourier transform infrared (FTIR) hyperspectral imaging, a method widely used in two-dimensional analysis of chemical components, to study HLA class I expression in tissue. Two-dimensional cluster colour images derived from unsupervised hierarchical cluster analysis of FTIR hyperspectral data on melanoma sections were compared with consecutive sections that were immunohistochemically stained for class I expression. HLA-class-I-positive and -negative areas were differentiated by FTIR cluster images in all eight melanoma sections investigated without the need for antibody attachment. FTIR imaging enables the distinction of HLA-class-I-positive from class-I-negative areas in melanoma. This method is accurate, rapid and cost-effective.
Exposure to ozone alters regional function and particle dosimetry in the human lung (1993)

Effects of experimental exposure to O3 (0.33 ppm) or filtered air on regional lung function were assessed in nine healthy male subjects. Immediately after 2-h chamber exposures, regional ventilation and particle dosimetry were measured by gamma camera imaging. The vertical distributions of a radiolabeled gas (133Xe) and aerosol (3.5-microns-diam insoluble 99mTc-tagged Fe2O3 particles) were quantitated for upper, middle, and lower lung regions; distribution data were corrected for regional differences in lung volume and tissue attenuation. Indexes of mechanical function, inspiratory capacity, and mid-maximal expiratory flow rates were significantly reduced after O3, but functional residual capacity remained unchanged. Exposure to O3 significantly enhanced the fraction of respired aerosol retained by the lung and altered the distribution pattern of deposited aerosol by increasing particle deposition to the middle lung region (P < 0.05). Aerosol penetration indexes, i.e., ratio of particle deposition in central lung regions to that in peripheral lung regions, and particle retention 24 h postinhalation (an index of aerosol deposition within alveoli and slowly clearing bronchioles) indicated that particle filtration efficiency had increased for tracheobronchial and parenchymal lung regions. For seven of the nine subjects, regional ventilation after O3 was reduced by 14% to the lung base and enhanced by 8 and 6% to the upper and middle lung regions, respectively; these changes were significant (P < 0.02) compared with ventilation after filtered air.(ABSTRACT TRUNCATED AT 250 WORDS)
Analysis of AC Impedance in Localized Region Using Magnetic Field Distribution Measured by HTS-SQUID (2018)

Estimation of ac impedance in a localized region was investigated by measuring the magnetic field from an electrical current induced by applying an ac voltage to a sample. For this measurement, a high-temperature-superconductor superconducting quantum interference device (HTS-SQUID) was used to detect a small magnetic field from a sample. To clarify the detection of ac impedance in a localized region, a dye-sensitized solar cell (DSSC) with a positive electrode consisting of two catalysis materials was prepared. Although the I-V characteristic and electrochemical impedance spectroscopy (EIS) of the solar cell with two catalysis materials were different from those of the solar cell with a one catalysis material, it was difficult to evaluate the ac impedance of the localized region. The magnetic field from the solar cell with two catalysis materials depended on the measurement position at which the catalysis material located under HTS-SQUID was different. This difference was correlated to the ac impedance change predicted by the measured EIS. Moreover, the mapping obtained by magnetic field distribution near the interface of two catalysis materials showed a different magnetic field intensity, which was correlated to the position of each catalysis material. These results indicate that the analysis of the localized ac impedance is possible using the magnetic field distribution generated from a sample.
The effects of quadrature sampling imbalances on a phase difference analysis technique (2002)

The effects of amplitude and phase imbalances between the in-phase and quadrature channels of a digital radio frequency (RF) receiver on a phase analysis processing technique are studied. Particular attention is given to the effect that these errors have on the detection and accurate frequency resolution of simultaneous signals. It is shown that these error effects can appear to be identical to a simultaneous signal condition. Thus using this technique in a practical electronic warfare (EW) receiver could cause incorrect conclusions unless the amplitude separation of the signals is small. Simulation results and data collected from a 250 MHz, 8 b digital receiver are presented to show how these errors, along with other anomalies, affect the performance of phase analysis processing, and specifically how they affect detection and determination of simultaneous signals.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Nouveau détecteur de gaz hyperfréquence à modes de galerie (2009)

National audience
Electron-Rich Phenoxyl Mediators Improve Thermodynamic Performance of Electrocatalytic Alcohol Oxidation with an Iridium Pincer Complex (2020)

Electron-rich phenols, including α-<i>rac</i>-tocopherol <b>Ar</b><sup><b>1</b></sup><b>OH</b>, 2,4,6,-tri-<i>tert</i>-butylphenol <b>Ar</b><sup><b>3</b></sup><b>OH</b>, and butylated hydroxy-toluene <b>Ar</b><sup><b>4</b></sup><b>OH</b>, are effective electrochemical mediators for the electrocatalytic oxidation of alcohols by an iridium amido dihyride complex (PNP)Ir(H)<sub>2</sub> (IrN <b>1</b>, PNP = bis[2-diisopropylphosphino)ethyl]amide). Addition of phenol mediators leads to a decrease in the onset potential of catalysis from -0.65 V vs Fc<sup>+/0</sup> under unmediated conditions to -1.07 V vs Fc<sup>+/0</sup> in the presence of phenols. Mechanistic analysis suggests that oxidative turnover of the iridium amino trihydride (PNHP)Ir(H)<sub>3</sub> (IrH <b>2</b>, PNHP = bis[2-diisopropylphosphino)ethyl]amine) to IrN <b>1</b> proceeds through two successive hydrogen atom transfers (HAT) to 2 equiv of phenoxyl that are generated transiently at the anode. Isotope studies and comparison to known systems are consistent with initial homolysis of an Ir-H bond being rate-determining. Turnover frequencies up to 14.6 s<sup>-1</sup> and an average Faradaic efficiency of 93% are observed. The mediated system shows excellent chemoselectivity in bulk oxidations of 2-propanol and 1,2-benzenedimethanol in THF and is also viable in neat 2-propanol.
ChemInform Abstract: Total Synthesis of FK 506 and an FKBP Probe Reagent, (C8,C9‐13C2)‐FK 506. Chemistry and Biology of the Immunophilins and Their Immunosuppressive Ligands (1992)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Solution structure of robustoxin, the lethal neurotoxin from the funnel‐web spider Atrax robustus (1997)

The solution structure of robustoxin, the lethal neurotoxin from the Sydney funnel-web spider Atrax robustus, has been determined from 2D 1H NMR data. Robustoxin is a polypeptide of 42 residues cross-linked by four disulphide bonds, the connectivities of which were determined from NMR data and trial structure calculations to be 1-15, 8-20, 14-31 and 16-42 (a 1-4/2-6/3-7/5-8 pattern). The structure consists of a small three-stranded, anti-parallel beta-sheet and a series of interlocking gamma-turns at the C-terminus. It also contains a cystine knot, thus placing it in the inhibitor cystine knot motif family of structures, which includes the omega-conotoxins and a number of plant and animal toxins and protease inhibitors. Robustoxin contains three distinct charged patches on its surface, and an extended loop that includes several aromatic and non-polar residues. Both of these structural features may play a role in its binding to the voltage-gated sodium channel.
Discovery of [11C]MK-6884: A Positron Emission Tomography (PET) Imaging Agent for the Study of M4Muscarinic Receptor Positive Allosteric Modulators (PAMs) in Neurodegenerative Diseases (2020)

The measurement of receptor occupancy (RO) using positron emission tomography (PET) has been instrumental in guiding discovery and development of CNS directed therapeutics. We and others have investigated muscarinic acetylcholine receptor 4 (M4) positive allosteric modulators (PAMs) for the treatment of symptoms associated with neuropsychiatric disorders. In this article, we describe the synthesis, in vitro, and in vivo characterization of a series of central pyridine-related M4 PAMs that can be conveniently radiolabeled with carbon-11 as PET tracers for the in vivo imaging of an allosteric binding site of the M4 receptor. We first demonstrated its feasibility by mapping the receptor distribution in mouse brain and confirming that a lead molecule <b>1</b> binds selectively to the receptor only in the presence of the orthosteric agonist carbachol. Through a competitive binding affinity assay and a number of physiochemical properties filters, several related compounds were identified as candidates for in vivo evaluation. These candidates were then radiolabeled with <sup>11</sup>C and studied in vivo in rhesus monkeys. This research eventually led to the discovery of the clinical radiotracer candidate [<sup>11</sup>C]MK-6884.
Renal negative pressure treatment as a novel therapy for heart failure-induced renal dysfunction (2021)

Congestion is the primary pathophysiological lesion in most heart failure (HF) hospitalizations. Renal congestion increases renal tubular pressure, reducing glomerular filtration rate (GFR) and diuresis. Because each nephron is a fluid-filled column, renal negative pressure therapy (rNPT) applied to the urinary collecting system should reduce tubular pressure, potentially improving kidney function. We evaluated the renal response to rNPT in congestive HF. Ten anesthetized ∼80-kg pigs underwent instrumentation with bilateral renal pelvic JuxtaFlow catheters. GFR was determined by iothalamate clearance (mGFR) and renal plasma flow (RPF) by para-aminohippurate clearance. Each animal served as its own control with randomization of left versus right kidney to -30 mmHg rNPT or no rNPT. mGFR and RPF were measured simultaneously from the rNPT and no rNPT kidney. Congestive HF was induced via cardiac tamponade maintaining central venous pressure at 20-22.5 mmHg throughout the experiment. Before HF induction, rNPT increased natriuresis, diuresis, and mGFR compared with the control kidney (<i>P</i> < 0.001 for all). Natriuresis, diuresis, and mGFR decreased following HF (<i>P</i> < 0.001 for all) but were higher in rNPT kidney versus control (<i>P</i> < 0.001 for all). RPF decreased during HF (<i>P</i> < 0.001) without significant differences between rNPT treatments. During HF, the rNPT kidney had similar diuresis and natriuresis (<i>P</i> > 0.5 for both) and higher fractional excretion of sodium (<i>P</i> = 0.001) compared with the non-rNPT kidney in the no HF period. In conclusion, rNPT resulted in significantly increased diuresis, natriuresis, and mGFR, with or without experimental HF. rNPT improved key renal parameters of the congested cardiorenal phenotype.
Can Cellular Phototoxicity be Accurately Predicted on the Basis of Sensitizer Photophysics? (1999)

The phototoxicity of three structurally related photosensitizers (PS), deuteroporphyrin IX (DP) and monobromo (Br-DP) and dibromo (Br2-DP) derivatives, was studied in murine L1210 leukemia cells. These compounds were chosen on the basis of heavy-atom-induced differences in triplet yield, phi T, and lifetime, tau T, and used as tools to test a model for phototoxicity based on photophysical parameters. All three porphyrins were found to localize preferentially in the plasma membrane of L1210 cells by confocal fluorescence microscopy. A poor correlation was observed between the measured photodynamic efficacies of these PS and a model using photophysical parameters determined by laser flash photolysis in homogeneous solution. However, an excellent correlation was obtained when the same parameters measured directly in the cells were used. The biological microenvironment of the porphyrins in cells induces significant changes in the photophysics of the PS. Reduction in fluorescence yield, phi F, and phi T observed for Br2-DP in cell suspensions arises from self association of the molecule due to increased hydrophobicity and high local concentrations. The photophysical model was also tested for its ability to handle variations in the oxygen dependence of cellular phototoxicity of these PS. The good correlation achieved between laser flash photolysis data determined in cells and the measured phototoxicity under air, 1.5% and 0.5% O2-saturated conditions, proves the intermediacy of singlet oxygen. This study gives further credence to the direct use of photophysical techniques to elucidate photochemical mechanisms in biological media while highlighting the potential pitfalls of using solution data to predict photosensitizing potential.