gebhart/patrepeval huggingface dataset:
Video-based rendering (2005)

Article Share on Video-based rendering Authors: Marcus Magnor MPI Informatik MPI InformatikView Profile , Marc Pollefeys University of North Carolina at Chapel Hill University of North Carolina at Chapel HillView Profile , German Cheung Neven Vision Inc. Neven Vision Inc.View Profile , Wojciech Matusik Mitsubishi Electric Research Laboratory (MERL) Mitsubishi Electric Research Laboratory (MERL)View Profile , Christian Theobalt MPI Informatik MPI InformatikView Profile Authors Info & Claims SIGGRAPH '05: ACM SIGGRAPH 2005 CoursesJuly 2005 Pages 1–eshttps://doi.org/10.1145/1198555.1198678Online:31 July 2005Publication History 7citation1,228DownloadsMetricsTotal Citations7Total Downloads1,228Last 12 Months12Last 6 weeks2 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
Early age-related macular degeneration detection by focal biologically inspired feature (2012)

Age-related macular degeneration (AMD) is a leading cause of vision loss. The presence of drusen are often associated to AMD. Drusen are tiny yellowish-white extracellular buildup present around the macular region of the retina. Clinically, ophthalmologists examine the area around the macula to determine the presence and severity of drusen. However, manual identification and recognition of drusen is subjective, time consuming and expensive. To reduce manual workload and facilitate large-scale early AMD screening, it is essential to detect drusen automatically. In this paper, we propose to use biologically inspired features (BIF) for the purpose of AMD detection. The optic disc and macula are detected to determine a focal region around macula for feature extraction. The extracted features are then classified using support vector machines (SVM). Our experimental results, tested on 350 images, demonstrate that the biologically inspired features from the focal region is effective for drusen detection with a sensitivity of 86.3% and specificity of 91.9%. The results of our proposed approach can be used to reduce workload of ophthalmologists and diagnosis cost.
Molecular engineering of antibodies for site-specific covalent conjugation using CRISPR/Cas9 (2018)

Site-specific modification of antibodies has become a critical aspect in the development of next-generation immunoconjugates meeting criteria of clinically acceptable homogeneity, reproducibility, efficacy, ease of manufacturability, and cost-effectiveness. Using CRISPR/Cas9 genomic editing, we developed a simple and novel approach to produce site-specifically modified antibodies. A sortase tag was genetically incorporated into the C-terminal end of the third immunoglobulin heavy chain constant region (CH3) within a hybridoma cell line to manufacture antibodies capable of site-specific conjugation. This enabled an effective enzymatic site-controlled conjugation of fluorescent and radioactive cargoes to a genetically tagged mAb without impairment of antigen binding activity. After injection in mice, these immunoconjugates showed almost doubled specific targeting in the lung vs. chemically conjugated maternal mAb, and concomitant reduction in uptake in the liver and spleen. The approach outlined in this work provides a facile method for the development of more homogeneous, reproducible, effective, and scalable antibody conjugates for use as therapeutic and diagnostic tools.
Characteristics of EYFP-actin and visualization of actin dynamics during ATP depletion and repletion (2001)

Disruption of the actin cytoskeleton in proximal tubule cells is a key pathophysiological factor in acute renal failure. To investigate dynamic alterations of the actin cytoskeleton in live proximal tubule cells, LLC-PK(10) cells were transfected with an enhanced yellow fluorescence protein (EYFP)-actin construct, and a clone with stable EYFP-actin expression was established. Confluent live cells were studied by confocal microscopy under physiological conditions or during ATP depletion of up to 60 min. Immunoblots of stable transfected LLC-PK(10) cells confirmed the presence of EYFP-actin, accounting for 5% of total actin. EYFP-actin predominantly incorporated in stress fibers, i.e., cortical and microvillar actin as shown by excellent colocalization with Texas red phalloidin. Homogeneous cytosolic distribution of EYFP-actin indicated colocalization with G-actin as well. Beyond previous findings, we observed differential subcellular disassembly of F-actin structures: stress fibers tagged with EYFP-actin underwent rapid and complete disruption, whereas cortical and microvillar actin disassembled at slower rates. In parallel, ATP depletion induced the formation of perinuclear EYFP-actin aggregates that colocalized with F-actin. During ATP depletion the G-actin fraction of EYFP-actin substantially decreased while endogenous and EYFP-F-actin increased. During intracellular ATP repletion, after 30 min of ATP depletion, there was a high degree of agreement between F-actin formation from EYFP-actin and endogenous actin. Our data indicate that EYFP-actin did not alter the characteristics of the endogenous actin cytoskeleton or the morphology of LLC-PK(10) cells. Furthermore, EYFP-actin is a suitable probe to study the spatial and temporal dynamics of actin cytoskeleton alterations in live proximal tubule cells during ATP depletion and ATP repletion.
30 YEARS OF THE MINERALOCORTICOID RECEPTOR: Mineralocorticoid receptor antagonists: 60 years of research and development (2017)

The cDNA of the mineralocorticoid receptor (MR) was cloned 30 years ago, in 1987. At that time, spirolactone, the first generation of synthetic steroid-based MR antagonists (MRAs), which was identified in preclinical <i>in vivo</i> models, had already been in clinical use for 30 years. Subsequent decades of research and development by Searle & Co., Ciba-Geigy, Roussel Uclaf and Schering AG toward identifying a second generation of much more specific steroidal MRAs were all based on the initial 17-spirolactone construct. The salient example is eplerenone, first described in 1987, coincidentally with the cloning of MR cDNA. Its launch on the market in 2003 paralleled intensive drug discovery programs for a new generation of non-steroidal MRAs. Now, 30 years after the cDNA cloning of MR and 60 years of clinical use of steroidal MRAs, novel non-steroidal MRAs such as apararenone, esaxerenone and finerenone are in late-stage clinical trials in patients with heart failure, chronic kidney disease (CKD), hypertension and liver disease. Finerenone has already been studied in over 2000 patients with heart failure plus chronic kidney disease and/or diabetes, and in patients with diabetic kidney disease, in five phase II clinical trials. Here, we reflect on the history of the various generations of MRAs and review characteristics of the most important steroidal and non-steroidal MRAs.
CGRP Signaling via CALCRL Increases Chemotherapy Resistance and Stem Cell Properties in Acute Myeloid Leukemia (2019)

The neuropeptide CGRP, acting through the G-protein coupled receptor CALCRL and its coreceptor RAMP1, plays a key role in migraines, which has led to the clinical development of several inhibitory compounds. Recently, high <i>CALCRL</i> expression has been shown to be associated with a poor prognosis in acute myeloid leukemia (AML). We investigate, therefore, the functional role of the CGRP-CALCRL axis in AML. To this end, <i>in silico</i> analyses, human AML cell lines, primary patient samples, and a C57BL/6-based mouse model of AML are used. We find that <i>CALCRL</i> is up-regulated at relapse of AML, in leukemic stem cells (LSCs) versus bulk leukemic cells, and in LSCs versus normal hematopoietic stem cells. CGRP protects receptor-positive AML cell lines and primary AML samples from apoptosis induced by cytostatic drugs used in AML therapy, and this effect is inhibited by specific antagonists. Furthermore, the CGRP antagonist olcegepant increases differentiation and reduces the leukemic burden as well as key stem cell properties in a mouse model of AML. These data provide a basis for further investigations into a possible role of CGRP-CALCRL inhibition in the therapy of AML.
An Analysis of the Completion Time of the BB84 Protocol (2023)

The BB84 QKD protocol is based on the idea that the sender and the receiver can reconcile a certain fraction of the teleported qubits to detect eavesdropping or noise and decode the rest to use as a private key. Under the present hardware infrastructure, decoherence of quantum states poses a significant challenge to performing perfect or efficient teleportation, meaning that a teleportation-based protocol must be run multiple times to observe success. Thus, performance analyses of such protocols usually consider the completion time, i.e., the time until success, rather than the duration of a single attempt. Moreover, due to decoherence, the success of an attempt is in general dependent on the duration of individual phases of that attempt, as quantum states must wait in memory while the success or failure of a generation phase is communicated to the relevant parties. In this work, we do a performance analysis of the completion time of the BB84 protocol in a setting where the sender and the receiver are connected via a single quantum repeater and the only quantum channel between them does not see any adversarial attack. Assuming certain distributional forms for the generation and communication phases of teleportation, we provide a method to compute the MGF of the completion time and subsequently derive an estimate of the CDF and a bound on the tail probability. This result helps us gauge the (tail) behaviour of the completion time in terms of the parameters characterising the elementary phases of teleportation, without having to run the protocol multiple times. We also provide an efficient simulation scheme to generate the completion time, which relies on expressing the completion time in terms of aggregated teleportation times. We numerically compare our approach with a full-scale simulation and observe good agreement between them.
Table S1, Table S2, table S3, and Table S4 from Combination Therapy with Bispecific Antibodies and PD-1 Blockade Enhances the Antitumor Potency of T Cells (2023)

&lt;p&gt;Combined file of Table S1 thru S4. Table S1. Blockade of PD-1 binding to PD-L1 on MDA-MB-231 by anti-PD-1. Table S2. Surface expression of CEACAM5 and Trop-2 on selective cell lines. Table S3. Expression of Trop-2 and PD-L1 on MDA-MB-231 and its Trop-2-transfected sublines, including the IC50 of (E1)-3s determined with T cells purified from PBMCs of the same donor. Table S4. Effect of cPD-1 on IC50/EC50 of (E1)-3s to kill MDA-MB-231 or MDA-MB-231-S120 with redirected T cells.&lt;/p&gt;
Erratum: Chemical softness and impurity segregation at grain boundaries [J. Chem. Phys. 9 8, 1606 (1993)] (1993)

is the external potentia1.
Membrane-proximal binding of STAT3 revealed by cancer-associated receptor variants (2016)

In cancer biology, somatic mutations in the extracellular (ligand binding) and cytosolic (functional/catalytic) domains are pursued with great interest. However, in our recent publication we report that germline mutations in the membrane-proximal region of type I receptors are able to modulate the amplitude of signal transducer and activator of transcription 3 (STAT3) signaling in cells. This unexpected finding has implications for the prognosis of heritable cancer.
Three-step H2Se/Ar/H2S reaction of Cu-In-Ga precursors for controlled composition and adhesion of Cu(In,Ga)(Se,S)2 thin films (2012)

Control of the through-film composition and adhesion are critical issues for Cu(In,Ga)Se2 (CIGS) and/or Cu(In,Ga)(Se,S)2 (CIGSS) films formed by the reaction of Cu–In–Ga metal precursor films in H2Se or H2S. In this work, CIGSS films with homogenous Ga distribution and good adhesion were formed using a three-step reaction involving: (1) selenization in H2Se at 400 °C for 60 min, (2) temperature ramp-up to 550 °C and annealing in Ar for 20 min, and (3) sulfization in H2S at 550 °C for 10 min. The 1st selenization step led to fine grain microstructure with Ga accumulation near the Mo back contact, primarily in a Cu9(In1−xGax)4 phase. The 2nd Ar anneal step produces significant grain growth with homogenous through-film Ga distribution and the formation of an InSe binary compound near the Mo back contact. The 3rd sulfization step did not result in any additional change in Ga distribution or film microstructure but a small S incorporation near the CIGSS film surface and complete reaction of InSe to form CIGSS were observed. The three-step process facilitates good control of the film properties by separating different effects of the reaction process and a film growth model is proposed. Finally, CIGSS solar cells with the three-step reaction were fabricated and devices with efficiency = 14.2% and VOC = 599 mV were obtained.
The role of cytokines in angiogenesis (1993)

Using the corneal model of neovascularization developed in their laboratory, the authors investigated the angiogenic potential of interleukin-1 (IL-1α and β), interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor (TNFα), transforming growth factor β (TGFβ), granulocyte macrophage-colony stimulating factor (GM-CSF), and interferon (IFN(γ) and IFN(γ)). Various concentrations of the tested cytokine were sequestered into Elvax-40 and implanted at 2.5 mm from the limbus within the corneal stroma of the rabbit eye. Three distinct groups of cytokines could be observed according to their angiogenic potential in this system. IL-1α was by far the most potent stimulator of neovascularization, inducing this process at concentrations as low as one nanogram per implant. TGFβ, TNFα and GM-CSF induced significant neovascularization only at concentrations of 500 nanograms per implant. IL-2, IL-6, IL-8, IFNα and IFN(γ) did not induce any significant neovascularization at the concentration tested (0.5 μg to 10 μg per implant).
Molecular scanning of the beta-3-adrenergic receptor gene in Pima Indians and Caucasians (1999)

The g1856t polymorphism is in linkage disequilibrium with the Trp64Arg variant, but does not appear to have a functional role.
Generation of Mice Deficient for Macrophage Galactose- and N-Acetylgalactosamine-Specific Lectin: Limited Role in Lymphoid and Erythroid Homeostasis and Evidence for Multiple Lectins (2002)

Macrophage receptors function in pattern recognition for the induction of innate immunity, in cellular communication to mediate the regulation of adaptive immune responses, and in the clearance of some glycosylated cells or glycoproteins from the circulation. They also function in homeostasis by initiating the engulfment of apoptotic cells. Evidence has suggested that macrophage receptors function to recognize cells that are destined for programmed cell death but not yet overtly apoptotic. We have examined the function of a macrophage receptor specific for unsialylated glycoproteins, known as the mouse macrophage galactose- and N-acetylgalactosamine-specific lectin (mMGL) (Ii et al., J. Biol. Chem. 265:11295-11298, 1990; Sato et al., J. Biochem. [Tokyo] 111:331-336, 1992; Yamamoto et al., Biochemistry 33:8159-8166, 1994). With targeted disruption, we tested whether mMGL is necessary for macrophage function, controlled thymic development, the loss of activated CD8 T cells, and the turnover of red blood cells. Evidence indicates that mMGL may play a nonessential role in several of these macrophage functions. Experiments are presented that indicate the existence of another galactose- and N-acetylgalactosamine-recognizing lectin distinct from mMGL. This may explain the absence of a strong phenotype in mMGL-deficient mice.
Smoking negatively impacts renal cell carcinoma overall and cancer‐specific survival (2011)

In patients with RCC, a history of smoking was associated with worse pathologic features and survival outcomes and with an increased risk of having mutated p53. Further investigation of the genetic and molecular mechanisms associated with decreased CSS in patients with RCC who have a history of smoking is indicated.
A Macrophage Cell Model for Selective Metalloproteinase Inhibitor Design (2008)

The desire to inhibit zinc-dependent matrix metalloproteinases (MMPs) has, over the course of the last 30 years, led to the development of a plethora of MMP inhibitors that bind directly to the active-site metal. With one exception, all of these drugs have failed in clinical trials, due to many factors, including an apparent lack of specificity for MMPs. To address the question of whether these inhibitors are selective for MMPs in a biological setting, a cell-based screening method is presented to compare the relative activities of zinc, heme iron, and non-heme iron enzymes in the presence of these compounds using the RAW264.7 macrophage cell line. We screened nine different zinc-binding groups (ZBGs), four established MMP inhibitors (MMPis), and two novel MMP inhibitors developed in our laboratory to determine their selectivities against five different metalloenzymes. Using this model, we identified two nitrogen donor compounds--2,2'-dipyridylamine (DPA) and triazacyclononane (TACN)--as the most selective ZBGs for zinc metalloenzyme inhibitor development. We also demonstrated that the model could predict known nonspecific interactions of some of the most commonly used MMPis, and could also give cross-reactivity information for newly developed MMPis. This work demonstrates the utility of cell-based assays in both the design and the screening of novel metalloenzyme inhibitors.
Impact of Al<sub>2</sub>O<sub>3</sub> Passivation on AlGaN/GaN Nanoribbon High-Electron-Mobility Transistors (2015)

Passivation films are used in III-nitride (III-N) based devices to suppress current collapse and improve frequency performance. Several passivation films and deposition methods have the added effects of increasing the dc ON- and OFF-state currents in devices. In this paper, the physical mechanisms behind this current increase have been studied in both nanoribbon and planar devices with atomic-layer deposited Al <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> O <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> passivation. Increased tensile stress in the AlGaN layer due to passivation leads to an increase in the charge density in nanoribbon devices. Simultaneously, the mobility in nanoribbons increases after Al <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> O <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> passivation. These effects lead to a large (~118%) increase in the saturation drain current in nanoribbon devices. In contrast, fixed positive charge at the Al <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> O <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> -AlGaN interface leads to a small (~6%) saturation drain current increase in planar devices. In addition, the mechanisms behind the increase in the OFF-state drain current in the passivated devices are investigated. Schottky barrier lowering and the increase in surface and buffer conduction are found to be the major causes for the OFF-state current increase with passivation.
Sensor gain and noise requirements for fluoroscopic applications (2001)

The low x-ray exposures used in fluoroscopic applications (0.1 - 10mR at the sensor surface) mean that the requirements for sensor gain and noise are particularly strict. The achievable DQE is determined by a number of factors, including the sensor quantum efficiency, x-ray absorption Swank factor, secondary quanta conversion efficiency, internal gain (e.g. the number of electrons collected per visible photon produced in the phosphor), and additive noise. The influence of these factors is examined for three direct detection x-ray sensors (PbI2, a-Se and GaAs), and one indirect detector sensor (CsI). Although the characteristics of these sensors are very different, it is demonstrated that all are appropriate for use in fluoroscopic applications as a replacement for current image intensifier based systems.
Identifying protective Streptococcus pyogenes vaccine antigens recognized by both B and T cells in human adults and children (2016)

No commercial vaccine exists against Group A streptococci (GAS; Streptococcus pyogenes) and only little is known about anti-GAS protective immunity. In our effort to discover new protective vaccine candidates, we selected 21 antigens based on an in silico evaluation. These were all well-conserved among different GAS strains, upregulated in host-pathogen interaction studies, and predicted to be extracellular or associated with the surface of the bacteria. The antigens were tested for both antibody recognition and T cell responses in human adults and children. The antigenicity of a selected group of antigens was further validated using a high-density peptide array technology that also identified the linear epitopes. Based on immunological recognition, four targets were selected and tested for protective capabilities in an experimental GAS infection model in mice. Shown for the first time, three of these targets (spy0469, spy1228 and spy1801) conferred significant protection whereas one (spy1643) did not.
A Survey on Open-source Cloud Computing Solutions (2010)

Cloud computing is an attractive computing model since it allows for resources to be provisioned according on a demand basis, i.e., cloud users can rent resources as they become necessary. This model motivated several academic and non-academic institutions to develop open-source cloud solutions. This paper presents and discusses the state-of-the of open-source solutions for cloud computing. The authors hope that the observation and classification of such solutions can leverage the cloud computing research area providing a good starting point to cope with some of the problems present in cloud computing environments.