gebhart/patrepeval huggingface dataset:
Abstract 4153: CRISPR/Cas9-based oncogene editing enables characterization of rare mutations in cancer cells (2017)

Abstract In the past decade new insights into key oncogenic pathways and the development of targeted therapies enabled the success of precision medicine. However, only a small part of the cancer genome can be targeted effectively. This is in part due to the fact that the number of cancer cell models for a given oncogenic mutation is limited. We hypothesized that CRISPR/Cas9-mediated genome editing may enhance the ability to functionally study rare genomic lesions or resistance mutations that arise in patients under therapy. As a proof of concept we established, genetically modified PC9 lung cancer cells that harbor an activating EGFR ex19 deletion (EGFRdel E746-A750) into double mutated (EGFRdel E746-A750+T790M) or triple mutated (EGFRdel E746-A750+T790M+C797S) EGFR in order to induce resistance against known EGFR inhibitors. These cell lines fully recapitulated the phenotypic response pattern to first and third generation EGFR inhibitors that have been observed in patients harboring these clinically relevant resistance mutations. In a next step we switched the dependency of PC9 cells from the intrinsic oncogenic EGFR mutation into a rare ex20 SVDins mutation (EGFRD770_N771insSVD) that is typically associated with resistance towards all known EGFR inhibitors. Again, we observed a shift in the response to targeted inhibition of EGFR when compared to parental cells. Since the methodological principles can be applied to an array on known oncogenic mutations our approach may serve as a versatile platform for the functional study of oncogenic mutations that are found in patients. We envision that such reprogrammed cells may be of use for the screening of novel targeted therapeutics and therefore may enable the development of mutation specific drugs that are currently lacking for the majority of patients. Citation Format: Dennis Plenker, Martin L. Sos. CRISPR/Cas9-based oncogene editing enables characterization of rare mutations in cancer cells [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 4153. doi:10.1158/1538-7445.AM2017-4153
Multicomponent T 1 Relaxometry Analysis using Quantitative Parameter Mapping Magnetic Resonance Imaging. (2020)

Poster: ECR 2020 / C-08767 / Multicomponent T1 Relaxometry Analysis using Quantitative Parameter Mapping Magnetic Resonance Imaging. by: N. Maeda 1, Y. Kanazawa1, M. Harada1, Y. Taniguchi2, Y. Matsumoto3, T. Abe1, H. Hayashi4, M. Ono2, Y. Bito2; 1Tokushima/JP, 2Tokyo/JP, 3Tokushima-shi/JP, 4Kanazawa/JP
Flexible, high-resolution micro-optical coherence tomography endobronchial probe toward in vivo imaging of cilia (2017)

We report the design and fabrication of a flexible, longitudinally scanning high-resolution micro-optical coherence tomography (μOCT) endobronchial probe, optimized for micro-anatomical imaging in airways. The 2.4 mm diameter and flexibility of the probe allows it to be inserted into the instrument channel of a standard bronchoscope, enabling real-time video guidance of probe placement. To generate a depth-of-focus enhancing annular beam, we utilized a new fabrication method, whereby a hollow glass ferrule was angle-polished and gold-coated to produce an elongated annular reflector. We present validation data that verifies the preservation of linear scanning, despite the use of flexible materials. When utilized on excised, cultured mouse trachea, the probe acquired images of comparable quality to those obtained by a benchtop μOCT system.
Precursor Homogeneity and Crystallization Effects in Chemical Solution Deposition‐Derived Alkaline Niobate Thin Films (2011)

Chemical solution deposition‐derived potassium niobate and sodium niobate thin films have been prepared under protective atmosphere using an alkoxide approach. A profound dependency of the quality and homogeneity of the resulting thin films on the preparation of the alkaline precursor has been found. In addition to this, an additional increase in microstructure homogeneity for the significantly more sensitive potassium niobate system is obtainable if the precursor solution is further stabilized by acetylacetone although ambient humidity is already strongly suppressed. The observed behavior is attributed to the stabilization of the heterometallic complex in the precursor solution until film deposition commenced. Further on, the crystallization pathway of the respective alkaline niobate thin films has been evaluated and indication of crystallization through a metastable phase has been provided which seems to play a key role in orientation control. Finally, the temperature dependency of the lattice parameter spacing for potassium niobate and sodium niobate thin films has been presented and evidence of a strong substrate‐induced lattice deformation has been given.
Synthesis of β-Iminoaminate Zirconium Complexes and Their Application in Ethylene Polymerization (2000)

ADVERTISEMENT RETURN TO ISSUECommunication to the...Communication to the EditorNEXTSynthesis of β-Iminoaminate Zirconium Complexes and Their Application in Ethylene PolymerizationXin Jin and Bruce M. NovakView Author Information Polymer Science and Engineering Department, University of Massachusetts, Amherst, Massachusetts 01003, and Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695 Cite this: Macromolecules 2000, 33, 17, 6205–6207Publication Date (Web):August 3, 2000Publication History Received11 January 2000Revised11 July 2000Published online3 August 2000Published inissue 1 August 2000https://pubs.acs.org/doi/10.1021/ma0000428https://doi.org/10.1021/ma0000428rapid-communicationACS PublicationsCopyright © 2000 American Chemical SocietyRequest reuse permissionsArticle Views391Altmetric-Citations32LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Aromatic compounds,Catalysts,Hydrocarbons,Ligands,Polymerization Get e-Alerts
Clinical trial design and end points for epidermal growth factor receptor-targeted therapies: implications for drug development and practice. (2003)

There is an increased perception that current chemotherapeutic drugs have almost reached a ceiling as systemic anticancer therapies. In great part because of their unpredictable side effects, the development of these cytotoxic drugs followed certain patterns and principles that may not necessarily
FineLine (2018)

Recovery is an intricate aspect of transaction processing architectures. In its traditional implementation, recovery requires the management of two persistent data stores---a write-ahead log and a materialized database---which must be carefully orchestrated to maintain transactional consistency. Furthermore, the design and implementation of recovery algorithms have deep ramifications into almost every component of the internal system architecture, from concurrency control to buffer management and access path implementation. Such complexity not only incurs high costs for development, testing, and training, but also unavoidably affects system performance, introducing overheads and limiting scalability. This paper proposes a novel approach for transactional storage and recovery called FineLine. It simplifies the implementation of transactional database systems by eliminating the log-database duality and maintaining all persistent data in a single, log-structured data structure. This approach not only provides more efficient recovery with less overhead, but also decouples the management of persistent data from in-memory access paths. As such, it blurs the lines that separate in-memory from disk-based database systems, providing the efficiency of the former with the reliability of the latter.
Group Event Detection with a Varying Number of Group Members for Video Surveillance (2015)

This paper presents a novel approach for automatic recognition of group activities for video surveillance applications. We propose to use a group representative to handle the recognition with a varying number of group members, and use an Asynchronous Hidden Markov Model (AHMM) to model the relationship between people. Furthermore, we propose a group activity detection algorithm which can handle both symmetric and asymmetric group activities, and demonstrate that this approach enables the detection of hierarchical interactions between people. Experimental results show the effectiveness of our approach.
Estimation of skull table thickness with clinical CT and validation with microCT (2014)

Abstract Brain injuries resulting from motor vehicle crashes (MVC) are extremely common yet the details of the mechanism of injury remain to be well characterized. Skull deformation is believed to be a contributing factor to some types of traumatic brain injury (TBI). Understanding biomechanical contributors to skull deformation would provide further insight into the mechanism of head injury resulting from blunt trauma. In particular, skull thickness is thought be a very important factor governing deformation of the skull and its propensity for fracture. Current computed tomography (CT) technology is limited in its ability to accurately measure cortical thickness using standard techniques. A method to evaluate cortical thickness using cortical density measured from CT data has been developed previously. This effort validates this technique for measurement of skull table thickness in clinical head CT scans using two postmortem human specimens. Bone samples were harvested from the skulls of two cadavers and scanned with microCT to evaluate the accuracy of the estimated cortical thickness measured from clinical CT. Clinical scans were collected at 0.488 and 0.625 mm in plane resolution with 0.625 mm thickness. The overall cortical thickness error was determined to be 0.078 ± 0.58 mm for cortical samples thinner than 4 mm. It was determined that 91.3% of these differences fell within the scanner resolution. Color maps of clinical CT thickness estimations are comparable to color maps of microCT thickness measurements, indicating good quantitative agreement. These data confirm that the cortical density algorithm successfully estimates skull table thickness from clinical CT scans. The application of this technique to clinical CT scans enables evaluation of cortical thickness in population‐based studies.
Therapeutic potential of benzothiazoles: a patent review (2010 – 2014) (2015)

Several molecules containing benzothiazole skeleton are agents of choice for the treatment of various human diseases/disorders. Its versatile character of being capable of serving as ligand to various biomolecules attracted the interest of medicinal chemists for the development of therapies for respective ailments, especially, the 2-arylbenzothiazole moiety which is under development for the treatment of cancer. This signifies the increasing importance of benzothiazole nucleus in the area of drug discovery. Its structural simplicity and ease of synthesis provides scope for the development of chemical libraries that could serve in the discovery of new chemical entities progressing towards the market.
A design strategy for phase synchronization in Precoding-enabled DVB-S2X user terminals (2021)

This paper address the design of a phase tracking block for the DVB-S2X user terminals in a satellite precoding system. The spectral characteristics of the phase noise introduced by the oscillator, the channel, and the thermal noise at the receiver are taken into account. Using the expected phase noise mask, the optimal parameters for a second-order PLL intended to track channel variations from the pilots are calculated. To validate the results a Simulink model was implemented considering the characteristics of the hardware prototype. The performance of the design was evaluated in terms of the accuracy and stability for the frame structure of superframe Format 2, as described in Annex E of DVB-S2X.
Autoreduction of Pd−Co and Pt−Co Cyanogels: Exploration of Cyanometalate Coordination Chemistry at Elevated Temperatures (2008)

Cyanogels are coordination polymers made from the reaction of a chlorometalate and a cyanometalate in aqueous solution, which undergo a sol-gel transition to form stable gels. At temperatures above 240 degrees C, the cyanide ligand acts as a reducing agent and reduces the metal centers to lower oxidation states. To understand the mechanism of the autoreduction, the thermal reduction of the Pd-Co cyanogel system formed by the reaction of PdCl4(2-) and Co(CN)6(3-) was studied in an inert atmosphere. It was found that the reduction proceeds through two polymeric cyanide-containing intermediates, CoPd(CN)4 and Pd(CN)2, that form upon reduction of Co(3+) to Co(2+) and involves a significant rearrangement of the coordination structure. The two intermediates upon further heating reduce to metallic products, which by solid-state diffusion form a single Pd/Co alloy product. CoPd(CN)4 was found to have a hydrated form Co(H2O)2Pd(CN)4 x 4 H2O with a layered structure crystallizing in an orthorhombic Pnma space group. The Pt-Co cyanogel was found to autoreduce via a similar route. CoPt(CN)4 was confirmed as an intermediate. Understanding of the mechanism of the cyanogel autoreduction is an important step toward better understanding of opportunities that cyanogels offer in materials chemistry, as well as an expansion of the knowledge of coordination chemistry at elevated temperatures in general.
Cancer mediates effector T cell dysfunction by targeting microRNAs and EZH2 via glycolysis restriction (TUM9P.1007) (2015)

Abstract Aerobic glycolysis regulates T cell function. However, if and how primary cancer alters effector T cell glycolytic metabolism and affects tumor immunity remains a question in cancer patients. Here we report that ovarian cancer imposes glucose restriction on effector T cells, dampens their function via maintaining high expression of microRNA101 and microRNA26a, which subsequently constrains EZH2 expression. EZH2 activates the Notch pathway by suppressing Notch repressors via H3K27me3, and consequently stimulates T cell polyfunctional cytokine expression and promotes their survival via Bcl-2 signaling. Moreover, EZH2-/- T cells elicit poor anti-tumor immunity and EZH2+CD8+ T cells are associated with improved long-term cancer patient survival. Together, our data unveil a novel metabolic target and mechanism of cancer immune evasion.
Lewis Acid-Driven Accelerated Living Polymerization of Lactones Initiated with Aluminum Porphyrins. Chemoselective Activation of Ester Groups by Lewis Acid (1997)

Ring-opening polymerization of δ-valerolactone (δ-VL) initiated with (5,10,15,20-tetraphenylporphinato)aluminum methoxide ((TPP)AlOMe, 1; X = OMe) was dramatically accelerated upon addition of methylaluminum bis(2,6-di-tert-butyl-4-methylphenolate) (2a) and bis(2,4,6-tri-tert-butylphenolate) (2b) via coordinative activation of monomer, but was accompanied by transesterification, resulting in broadening of the polymer molecular weight distribution (MWD) at the later stage of polymerization. On the contrary, when methylaluminum bis(2,6-di-X-phenolate)s (X = Cl (2c), Br (2d), I (2e), and Ph (2f)) were used as Lewis acids, the polymerization proceeded also rapidly, retaining the narrow MWD. 13C NMR studies on the Lewis acid (2b or 2f)−δ-VL and −poly(δ-VL) mixtures showed that the coordination abilities of 2b and 2f toward the monomer ester group are comparable to each other, while 2f shows a lower affinity to the polymer ester group than 2b. 2a was also able to accelerate the polymerization of β-butyrolactone (β-BL) with (TPP)AlOMe or (5,10,15,20-tetraphenylporphinato)aluminum chloride ((TPP)AlCl, 1; X = Cl) to different extents depending on the mode of ring scission, where the MWD of the polymer remained narrow throughout the accelerated polymerization (Mw/Mn = 1.1−1.2).
High stiffness control of direct-drive motor system by a homogeneous ER fluid (2003)

Since a direct-drive (DD) motor system does not have the reduction gear, its structure is simple and has stiffness. In the DD motor system, the disturbance torque directly influences the control accuracy. The high servo stiffness is effective and important for reducing the influence of the disturbance torque. However, the high servo control often makes the system vibratory or unstable. Therefore, the servo gain must be lowered for assuring stability, and the influence of the disturbance becomes serious. In this study, the DD motor system using an electro-rheological (ER) fluid damper is developed. By applying the ER damper to the DD motor system, the gain margin of the control system is improved, so it is possible to realize the high servo control. Finally, it is experimentally shown that the proposed system is effective for high-speed and high-precision control.
Chiroptical transmission through a plasmonic helical traveling-wave nanoantenna, towards on-tip chiroptical probes (2019)

Resonant plasmonic helices have been widely utilized for locally enhancing and tailoring optical chirality. Here we investigate their nonresonant operation through the recently introduced concept of a plasmonic helical "traveling-wave" nanoantenna. Relying on the coupling of a nonresonant plasmonic helix and a nano-aperture, the helical traveling-wave nanoantenna transmits circularly polarized light with the same handedness as the helix and blocks the other, with a measured dissymmetry factor larger than 1.92 (maximum value of 2). This chiroptical transmission is spatially localized, spectrally broadband, and background-free. Finally, we demonstrate the possibility to engineer such a plasmonic helical nanoantenna at the apex of a sharp tip typically used in scanning near-field microscopies, thus opening the route for moveable, broadband, and background-free chiroptical probes.
Large strain variable stiffness composites for shear deformations with applications to morphing aircraft skins (2008)

Morphing or reconfigurable structures potentially allow for previously unattainable vehicle performance by permitting several optimized structures to be achieved using a single platform. The key to enabling this technology in applications such as aircraft wings, nozzles, and control surfaces, are new engineered materials which can achieve the necessary deformations but limit losses in parasitic actuation mass and structural efficiency (stiffness/weight). These materials should exhibit precise control of deformation properties and provide high stiffness when exercised through large deformations. In this work, we build upon previous efforts in segmented reinforcement variable stiffness composites employing shape memory polymers to create prototype hybrid composite materials that combine the benefits of cellular materials with those of discontinuous reinforcement composites. These composites help overcome two key challenges for shearing wing skins: the resistance to out of plane buckling from actuation induced shear deformation, and resistance to membrane deflections resulting from distributed aerodynamic pressure loading. We designed, fabricated, and tested composite materials intended for shear deformation and address out of plane deflections in variable area wing skins. Our designs are based on the kinematic engineering of reinforcement platelets such that desired microstructural kinematics is achieved through prescribed boundary conditions. We achieve this kinematic control by etching sheets of metallic reinforcement into regular patterns of platelets and connecting ligaments. This kinematic engineering allows optimization of materials properties for a known deformation pathway. We use mechanical analysis and full field photogrammetry to relate local scale kinematics and strains to global deformations for both axial tension loading and shear loading with a pinned-diamond type fixture. The Poisson ratio of the kinematically engineered composite is ~3x higher than prototypical orthotropic variable stiffness composites. This design allows us to create composite materials that have high stiffness in the cold state below SMP <i>T</i><sub>g</sub> (4-14GPa) and yet achieve large composite shear strains (5-20%) in the hot state (above SMP <i>T</i><sub>g</sub>).
Supplemental Dataset 2 from AXL Inhibition Sensitizes Mesenchymal Cancer Cells to Antimitotic Drugs (2023)

&lt;p&gt;Supplemental Dataset 2. Kinome profile with 1000nM R428&lt;/p&gt;
Core Loss of MgZn Ferrite. (1999)

The effects of substitution of Mn2O3 for Fe2O3 Or CuO for MgO in MgZn ferrite have been studied in order to achieve low core loss, focusing on the microstructure, the initial permeability and the resistivity of MnZn, MnMgZn, and MnMgCuZn ferrites.Initial permeability and resistivity of both MnMgZn and MnMgCuZn ferrites are higher than those of MgZn ferrite. Intragrain pores were observed in all of these ferrites. The pore density increases with grain growth.The core loss of these ferrites consists of hysteresis loss and eddy current loss at frequencies lower than 120kHz. Hysteresis loss clearly depends on initial permeability because of magnetic anisotropy, grain size and pore density. It has been found that eddy current loss is related to the microstructure and not to the resistivity. It is suggested that the decrease in the amount of pores inside the grains is effective to lower hysteresis and eddy current losses.Substitution of Cu enables MgZn ferrite to be sintered at lower temperatures, and it leads to the reduction of the core loss because of the formation of fine microstructure having small grain and low pore density. The lowest core loss (320kW/m3 at 100°C, 100mT and 100kHz) was achieved in the case of 11mol% CuO material sintered at 1120°C.
Mechanisms of Electrochemical N2 Splitting by a Molybdenum Pincer Complex (2021)

Molybdenum complexes supported by tridentate pincer ligands are exceptional catalysts for dinitrogen fixation using chemical reductants, but little is known about their prospects for electrochemical reduction of dinitrogen. The viability of electrochemical N2 binding and splitting by a molybdenum(III) pincer complex, (pyPNP)MoBr3 (pyPNP = 2,6-bis(tBu2PCH2)-C5H3N)), is established in this work, providing a foundation for a detailed mechanistic study of electrode-driven formation of the nitride complex (pyPNP)Mo(N)Br. Electrochemical kinetic analysis, optical and vibrational spectroelectrochemical monitoring, and computational studies point to two reaction pathways: in the “reaction layer” pathway, the molybdenum(III) precursor is reduced by 2e– and generates a bimetallic molybdenum(I) Mo2(-N2) species capable of N–N bond scission. In the “bulk solution” pathway the precursor is reduced by 3e– at the electrode surface to generate molybdenum(0) species that undergo chemical redox reactions via comproportionation in the bulk solution away from the electrode surface to generate the same bimetallic molybdenum(I) species capable of N2 cleavage. The comproportionation reactions reveal the surprising intermediacy of dimolybdenum(0) complex trans,trans-[(pyPNP)Mo(N2)2](-N2) in N2 splitting pathways. The same “over-reduced” molybdenum(0) species was also found to cleave N2 upon addition of lutidinium, an acid frequently used in catalytic reduction of dinitrogen.