gebhart/patrepeval huggingface dataset:
siRNA Down-regulation of the PATZ1 Gene in Human Glioma Cells Increases Their Sensitivity to Apoptotic Stimuli. (2008)

The PATZ1 gene encodes a transcription factor that belongs to the BTB/POZ group of transcriptional regulators and has been implicated as a transcriptional repressor. We cloned cDNA from glioma cell lines and found they expressed transcript variant 2 of PATZ1. We designed a specific siRNA against PATZ1 and showed that this siRNA, but not a control randomized siRNA, reduced PATZ1 expression in glioma cells as determined by quantitative PCR. In a panel of human glioma cell lines incubated with proapoptotic FasL, those transfected with PATZ1 siRNA displayed reduced cell numbers by the MTT colorimetric assay, relative to those transfected with randomized siRNA. Further studies showed that in 10-08-MG, U-251MG, U-87MG, and T98G cells PATZ1 siRNA significantly increased apoptosis in response to incubation with soluble FasL, as shown by a morphologic acridine orange/ethidium bromide apoptotic assay. Using an apoptosis specific cDNA microarray we further demonstrated that down-regulation of PATZ1 by siRNA resulted in the upregulation of death receptor pro-apoptotic genes including caspase 8 and Death Receptor 5 (DR5) in U-373MG cells. Since DR5 is the receptor for TRAIL we tested whether PATZ1 downregulation also sensitized cells to TRAIL-induced apoptosis and found that PATZ1 siRNA, but not control siRNA, sensitized U-251MG and T98G glioma cells to TRAIL-induced apoptosis. Altogether, these data demonstrate a previously unknown role for the transcription factor PATZ1 in conferring resistance to apoptosis and indicate that modulation of PATZ1 expression may be a therapeutic strategy for gliomas.
Supplementary Table 1 from Quantification of Excision Repair Cross-Complementing Group 1 and Survival in p16-Negative Squamous Cell Head and Neck Cancers (2023)

&lt;p&gt;PDF file - 78K, Characteristics of recurrent cases.&lt;/p&gt;
Development of electronic nose measurements for mango (Mangifera indica) homogenate and whole fruit (2004)

Mango fruit from Latin America (cv. Tommy Atkins), were purchased from a local Florida supermarket, homogenized, and sampled for volatile analysis by static headspace method. Some of the material was analyzed using an electronic nose (e-nose) with metal oxide coated or uncoated sensors (500 pL injection volume) and some by gas chromatography (GC) equipped with a polar Carbowax column and a flame ionization detector. Dilution of homogenate and homogenate vol-ume were analyzed to determine effect on e-nose and GC headspace measurements. Mango homogenate (1.0, 1.5, and 2.0 mL) was diluted with Dl water to 50, 25, and 12.5% of original concentration. The resulting a-nose signal intensities (changes in resistance across the metal oxide sensor due to non-selective interactions with volatile compounds in the headspace) were analyzed by discriminant factor analysis (DFA), which resulted in grouping by dilution factor, regardless of sample size. A combination of 2.0 mL and 25% dilution of mango homogenate was determined to be optimal. These results were com-pared to analysis of 13 characteristic mango volatiles by gas chromatography (GC) headspace analysis of the mango homogenate for the same volume/dilution combinations. Concentration of volatiles in the headspace generally increased with volume and decreased with dilution, but there were some exceptions and inconsistencies. The increase in headspace con-centration was not directly proportional to the homogenate volume, indicating matrix effects on aroma partitioning into the headspace, which varied for different compounds. Whole mangoes (cv. Keitt and Kent) harvested in Homestead, Fla., were put in sealed containers for 3 hours to accumulate enough vol-atiles for headspace analysis. A large injection volume injected into the e-nose (2000 pL) was necessary to get ample signal and reproducible results, and separated the two varieties based on their volatile emission to the headspace.
Evaluation of orthostatic hypotension using power spectral analysis. (1993)

To evaluate the pathogenesis of orthostatic hypotension, we studied the autonomic regulation system by measuring heart rate variability during 60 degrees passive head-up tilt using power spectral analysis in 21 patients with orthostatic hypotension (mean age 62 +/- 2 years, five with histories of cerebrovascular accidents, five with Parkinsonism, five with diabetes mellitus, three with pheochromocytoma, and three with unknown causes) and 15 normal healthy subjects as a control (mean age 63 +/- 2 years). We also assessed plasma epinephrine and norepinephrine response to tilt. During tilt, control subjects showed an increase in heart rate with no change in blood pressure. Spectral analysis of heart rate variability demonstrated increases in the low frequency band (LFB, mainly sympathetic) and low frequency band/high frequency band ratio (LFB/HFB, sympatho-vagal balance). All patients with orthostatic hypotension showed a significant reduction in blood pressure with an increase in heart rate. In patients with histories of cerebrovascular accidents and with Parkinsonism, LFB and the LFB/HFB ratio did not increase. However, in other patients with orthostatic hypotension, LFB and the LFB/HFB ratio increased during tilt. Norepinephrine increased in control subjects and in patients with diabetes mellitus, pheochromocytoma, and unknown causes. In contrast, patients with histories of cerebrovascular accidents and patients with Parkinsonism showed no increase in norepinephrine. Epinephrine responses paralleled those of norepinephrine, but the changes were not significant. Thus, neurological response to tilt is not uniform in patients with orthostatic hypotension. Patients with histories of cerebrovascular accidents and patients with Parkinsonism may have impaired function of central neural mechanisms controlling blood pressure.(ABSTRACT TRUNCATED AT 250 WORDS)
Influence of Compressive Torsion Processing Temperature on Microstructure Refinement and Property of Aluminum Alloy (2007)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.
Optoelectronic Fuzzy ARTMAP Processor (1995)

The realization of practical optical or optoelectronic computers has been hampered by the lack of algorithms suited to optoelectronic implementation. We have chosen an algorithm that is particularly compatible with optoelectronic processors and parallel access optical memory, mapped it onto an architecture which satisfies the constraints of the hardware, and suggest an implementation which is an appropriate combination of optical and electronic technology. The proposed parallel optoelectronic implementation increases throughput by several orders of magnitude over serial implementations, facilitating the real-time solution of large problems.
Non-rigid calcium phosphate cement containing hydrogel microbeads and absorbable fibres seeded with umbilical cord stem cells for bone engineering (2012)

The need for bone repair has increased as the population ages. Non-rigid calcium phosphate scaffolds could provide compliance for micro-motions within the tissues and yet have load-supporting strength. The objectives of this study were to: (a) develop a non-rigid calcium phosphate cement (CPC) with microbeads and fibre reinforcement; and (b) investigate human umbilical cord mesenchymal stem cell (hUCMSC) proliferation, osteodifferentiation and mineralization on non-rigid CPC for the first time. Non-rigid CPC was fabricated by adding extra tetracalcium phosphate in the traditional CPC and by incorporating chitosan, absorbable fibres and hydrogel microbeads. The non-rigid CPC-microbead scaffold possessed a strain-at-failure of 10.7%, much higher than the traditional CPC's strain of 0.05% which is typical for brittle bioceramics. Flexural strength of non-rigid CPC-microbead was 4-fold that of rigid CPC-microbead scaffold, while work-of-fracture (toughness) was increased by 20-fold. The strength of non-rigid CPC-microbead-fibre scaffold matched that of cancellous bone. hUCMSCs on non-rigid CPC proliferated from 100 cells/mm(2) at 1 day to 600 cells/mm(2) at 8 days. Alkaline phosphatase, osteocalcin and collagen gene expressions of hUCMSCs were greatly increased, and the cells synthesized bone minerals. hUCMSCs on non-rigid CPC-microbead-fibre constructs had higher bone markers and more mineralization than those on rigid CPC controls. In conclusion, this study developed the first non-rigid, in situ-setting calcium phosphate-microbead-fibre scaffold with a strain-at-failure exceeding 10%. hUCMSCs showed excellent proliferation, osteodifferentiation and mineralization on non-rigid CPC scaffold. The novel non-rigid CPC-hUCMSC construct with good strength, high strain-at-failure and toughness, as well as superior stem cell proliferation, osteodifferentiation and mineralization, is promising for load-bearing bone regeneration applications.
Impact of receiver structure and timing offset on MIMO spatial multiplexing (2008)

Multiple antenna enhancements via symbol timing relative offsets (MAESTRO) was recently introduced as a scheme to improve the performance of a V-BLAST like multi-antenna system by introducing sub-symbol timing offsets between the transmit antennas. In this paper we address the role of receiver structure on performance and investigate the optimal choice of the time offset parameter. A similar timing offset V-BLAST scheme was also introduced by Shao et al., where the authors use a zero-forcing (ZF) receiver and conclude the system outperforms V-BLAST only for small block sizes. We show that the zero-forcing (ZF) receiver suggested by Shao et al. is deficient and is the main reason for some of the drawbacks observed by them. The optimal ZF receiver is derived, and along with the linear MMSE receiver, is shown to exhibit no such weaknesses. The problem of optimal offset is also examined and the optimal offset for a 2 times 2 MIMO system derived.
Dynamic sympathetic regulation of left ventricular contractility studied in the isolated canine heart (1998)

We investigated the dynamic sympathetic regulation of left ventricular end-systolic elastance ( E es ) using an isolated canine ventricular preparation with functioning sympathetic nerves intact. We estimated the transfer function from both stellate ganglion stimulation to E es and ganglion stimulation to heart rate (HR) for both left and right ganglia by means of the white noise approach and transformed those transfer functions into corresponding step responses. The HR response was much larger with right sympathetic stimulation than with left sympathetic stimulation (4.3 ± 1.4 vs. 0.7 ± 0.6 beats ⋅ min −1 ⋅ Hz −1 , P &lt; 0.01). In contrast, the E es responses without pacing were not significantly different between left and right sympathetic stimulation (0.72 ± 0.34 vs. 0.76 ± 0.42 mmHg ⋅ ml −1 ⋅ Hz −1 ). Fixed-rate pacing significantly decreased the E es response to right sympathetic stimulation (0.53 ± 0.43 mmHg ⋅ ml −1 ⋅ Hz −1 , P &lt; 0.01), but not to left sympathetic stimulation (0.67 ± 0.32 mmHg ⋅ ml −1 ⋅ Hz −1 , not significant). Although the mechanism by which the sympathetic nervous system regulates cardiac contractility is different depending on whether the left or right sympathetic nerves are activated, this difference does not affect the apparent response of E es to dynamic sympathetic stimulation.
Portal vein thrombosis during liver transplantation: The risk of extra‐anatomical portal vein reconstruction (2020)

Grade IV thrombosis and extra-anatomical reconstruction were related to poor prognosis. Therefore, thorough evaluation and planning is required for these patients to improve the outcome.
P3‐291: Putative gamma secretase modulators lower Aβ42 in multiple in vitro and in vivo models (2009)

Inhibition of gamma secretase (GS) has been the focus of multiple drug discovery efforts over the last decade. However, the successful identification of potent gamma secretase inhibitors (GSIs) also led to the discovery of unacceptable side effects associated with this mechanism, i.e., the concomitant inhibition of Notch processing by GS. The observation that some non-steroidal anti-inflammatory drugs (NSAIDs) selectively inhibit the production of toxic Aβ42 while sparing Aβ and Notch has prompted the development of a class of compounds commonly referred to as gamma secretase modulators (GSMs). Using solid phase extraction in combination with an ELISA assay that utilized antibodies selective for Aβ42, Aβ40, Aβ38, or total Aβ peptides, this study describes presumptive GSMs that lower Aβ42 without altering total Aβ in a HEKswe_APP cell line, rat cortical cultures, and normal mice or rats. Western blot analysis of HEK293 cells transfected with NotchΔE was used to quantify the effect of compounds on Notch processing. IC50 values for Aβ42 lowering in the HEKswe_APP cell line were between 100 nM and 50 μM . Cytotoxicity was examined and was separated from the IC50 for Aβ42 -lowering by 10-100 fold. In rat cortical cultures the compounds were 3-5 -fold less potent for Aβ42 lowering and displayed a wide range of potencies (400 nM-300 μM). The range of potencies for in vitro inhibition of Notch processing were no effect on processing at 30 uM to a large effect at 100 nM. Aβ42 lowering in vivo was explored in normal mouse and rat brain. Animals were administered a single dose of 30 mg/kg Of each compound and the effects on brain Aβ42 levels were examined in mice or rats at 3 or 4 hours, respectively. The degree of brain penetration and ability to lower Aβ42 differed for each of the compounds. Interestingly, the degree of Aβ42 lowering did not correlate with the degree of Aβ38 production. In conclusion, the pharmacodynamic effects of GSMs have been profiled and the results suggest that modulation of gamma secretase may represent a viable means of reducing Aβ42 while avoiding toxicities associated with gamma secretase inhibition.
Protocol for In Vitro Regeneration and Marker Glycoside Assessment in Swertia chirata Buch-Ham (2009)

We have developed a rapid in vitro propagation system, via axillary shoot formation from nodal explants of Swertia chirata Buch Ham. Culture medium supplemented with 2 mg/L. BAP is best for direct shoot regeneration initially formed adventitious buds from axils of the nodal explants after 30 days. The reduced BAP concentration 0.5 mg/L proliferate shoots effectively. Kept the number of hyperhydrated shoots to minimal and induced on an average 22-38 shoots per flask (4.3 cm average length). The regenerated shoots (5- to 6-m long) formed roots very well in Murashige and Skoog (MS) medium devoid of any growth regulator and followed by acclimatization of plants in pre-sterilized sand containing 1% Trichoderma viride and Azatobactor chrococcum as bioinoculants. The regenerated plants don't show any genomic alterations. This protocol also outlines procedure of assessment of marker iridoid glycosides (amarogentin and amaroswerin) from callus, roots, multiple shoots, regenerated plants, and mother plant. High propagation frequency, reproducibility of procedure, molecular, and phenotypic and chemical stability ensures the efficiency of the developed protocol.
Toward cataloguing all rice genes: large‐scale sequencing of randomly chosen rice cDNAs from a callus cDNA library (1994)

A large-scale sequence analysis of rice cDNA was performed for a library from rice callus cultured in a medium containing 1 p.p.m. of 2,4-dichlorophenoxyacetic acid. Random sequencing of 2778 cDNA clones generated 2259 non-redundant expressed sequence tags (ESTs). The strategy of sequencing cDNAs can yield quickly a large number of novel genes. After translation, 690 sequences showed a significant amino acid sequence similarity to sequences already known from PIR. The source of known proteins ranged from bacteria to human. In this report, the non-redundant set of 280 identified ESTs is analyzed in detail.
Application of a New Solid-Phase Resin: Benzamide ortho-Lithiation and the Synthesis of a Phthalide Library (1999)

The utility of new polytetrahydrofuran cross-linked polystyrene resins is demonstrated through the solid-phase synthesis of a phthalide library. The phthalides are synthesized via directed ortho-lithiation of resin bound benzamides and isolated via cyclative cleavage.
Transcriptional Regulation of Chemical Diversity in Aspergillus fumigatus by LaeA (2007)

Secondary metabolites, including toxins and melanins, have been implicated as virulence attributes in invasive aspergillosis. Although not definitively proved, this supposition is supported by the decreased virulence of an Aspergillus fumigatus strain, DeltalaeA, that is crippled in the production of numerous secondary metabolites. However, loss of a single LaeA-regulated toxin, gliotoxin, did not recapitulate the hypovirulent DeltalaeA pathotype, thus implicating other toxins whose production is governed by LaeA. Toward this end, a whole-genome comparison of the transcriptional profile of wild-type, DeltalaeA, and complemented control strains showed that genes in 13 of 22 secondary metabolite gene clusters, including several A. fumigatus-specific mycotoxin clusters, were expressed at significantly lower levels in the DeltalaeA mutant. LaeA influences the expression of at least 9.5% of the genome (943 of 9,626 genes in A. fumigatus) but positively controls expression of 20% to 40% of major classes of secondary metabolite biosynthesis genes such as nonribosomal peptide synthetases (NRPSs), polyketide synthases, and P450 monooxygenases. Tight regulation of NRPS-encoding genes was highlighted by quantitative real-time reverse-transcription PCR analysis. In addition, expression of a putative siderophore biosynthesis NRPS (NRPS2/sidE) was greatly reduced in the DeltalaeA mutant in comparison to controls under inducing iron-deficient conditions. Comparative genomic analysis showed that A. fumigatus secondary metabolite gene clusters constitute evolutionarily diverse regions that may be important for niche adaptation and virulence attributes. Our findings suggest that LaeA is a novel target for comprehensive modification of chemical diversity and pathogenicity.
Anisotropic carrier transport properties in layered cobaltate epitaxial films grown by reactive solid-phase epitaxy (2009)

Herein we report the anisotropic carrier transport properties of a layered cobaltate, NaxCoO2 epitaxial film grown on the m-plane of an α-Al2O3 substrate using reactive solid-phase epitaxy. Scanning and transmission electron microscopy analyses revealed that NaxCoO2 was heteroepitaxially grown with the CoO2 conducting planes inclined by ∼43° against the α-Al2O3 substrate surface. The electrical resistivity parallel to the CoO2 planes was ∼1/5 of the perpendicular one, but the parallel Seebeck coefficient was about twice as large as the perpendicular one. Hence, a higher thermoelectric efficiency in the cobaltates can be obtained within the CoO2 planes.
The glycomic effect of N-acetylglucosaminyltransferase III overexpression in metastatic melanoma cells. GnT-III modifies highly branched N-glycans (2018)

N-acetylglucosaminyltransferase III (GnT-III) is known to catalyze N-glycan "bisection" and thereby modulate the formation of highly branched complex structures within the Golgi apparatus. While active, it inhibits the action of other GlcNAc transferases such as GnT-IV and GnT-V. Moreover, GnT-III is considered as an inhibitor of the metastatic potential of cancer cells both in vitro and in vivo. However, the effects of GnT-III may be more diverse and depend on the cellular context. We describe the detailed glycomic analysis of the effect of GnT-III overexpression in WM266-4-GnT-III metastatic melanoma cells. We used MALDI-TOF and ESI-ion-trap-MS/MS together with HILIC-HPLC of 2-AA labeled N-glycans to study the N-glycome of membrane-attached and secreted proteins. We found that the overexpression of GnT-III in melanoma leads to the modification of a broad range of N-glycan types by the introduction of the "bisecting" GlcNAc residue with highly branched complex structures among them. The presence of these unusual complex N-glycans resulted in stronger interactions of cellular glycoproteins with the PHA-L. Based on the data presented here we conclude that elevated activity of GnT-III in cancer cells does not necessarily lead to a total abrogation of the formation of highly branched glycans. In addition, the modification of pre-existing N-glycans by the introduction of "bisecting" GlcNAc can modulate their capacity to interact with carbohydrate-binding proteins such as plant lectins. Our results suggest further studies on the biological function of "bisected" oligosaccharides in cancer cell biology and their interactions with carbohydrate-binding proteins.
Zoledronic Acid Is Superior to Pamidronate in the Treatment of Hypercalcemia of Malignancy: A Pooled Analysis of Two Randomized, Controlled Clinical Trials (2001)

Zoledronic acid is superior to pamidronate; 4 mg is the dose recommended for initial treatment of HCM and 8 mg for relapsed or refractory hypercalcemia.
GAD: A 12-GS/s CMOS 4-bit A/D converter for an equalized multi-level link (2003)

A 4-bit 12-GSample/sec A/D converter (GAD) has been fabricated in a 0.25-/spl mu/m CMOS process to investigate the design of an equalized multi-level link. Clocked differential amplifiers were used to sample the input, followed by high-speed comparators with current-summed offset cancellation. Input bandwidth was measured at 2.5 GHz. Eight 1.5-GSample/sec flash A/D converters were interleaved to achieve the aggregate sample rate.
The C Proteins of Human Parainfluenza Virus Type 1 (HPIV1) Control the Transcription of a Broad Array of Cellular Genes That Would Otherwise Respond to HPIV1 Infection (2008)

Human parainfluenza virus type 1 (HPIV1) is an important respiratory pathogen in children and the most common cause of viral croup. We performed a microarray-based analysis of gene expression kinetics to examine how wild-type (wt) HPIV1 infection altered gene expression in human respiratory epithelial cells and what role beta interferon played in this response. We similarly evaluated HPIV1-P(C-), a highly attenuated and apoptosis-inducing virus that does not express any of the four C proteins, and HPIV1-C(F170S), a less attenuated mutant that contains a single point mutation in C and, like wt HPIV1, does not efficiently induce apoptosis, to examine the role of the C proteins in controlling host gene expression. We also used these data to investigate whether the phenotypic differences between the two C mutants could be explained at the transcriptional level. Mutation or deletion of the C proteins of HPIV1 permitted the activation of over 2,000 cellular genes that otherwise would be repressed by HPIV1 infection. Thus, the C proteins profoundly suppress the response of human respiratory cells to HPIV1 infection. Cellular pathways targeted by the HPIV1 C proteins were identified and their transcriptional control was analyzed using bioinformatics. Transcription factor binding sites for IRF and NF-kappaB were overrepresented in some of the C protein-targeted pathways, but other pathways were dominated by less-known factors, such as forkhead transcription factor FOXD1. Surprisingly, the host responses to the P(C-) and C(F170S) mutants were very similar, and only subtle differences in the expression kinetics of caspase 3 and TRAIL receptor 2 were observed. Thus, changes in host cell transcription did not reflect the striking phenotypic differences observed between these two viruses.