gebhart/patrepeval huggingface dataset:
Molecular Mechanism of Voltage-Dependent Ion Channels (1990)

Abstract : The focus of this research is the interaction of a molecule at the lipid/water interface of a membrane with the external electric field, using peptaibols and the S4 segment of the sodium channel as model systems. Experiments are underway to assess the role of the molecular dipole in sensing the imposed molecular field; the role of alpha helix-310-helix transitions in responding to the imposed molecular field as the transduction mechanism; and the role of the channel mouth in determining the ion selectivity associated with channel opening. Ac-(Lys(alamethicin 1-17)4-NH2, tetramethicin, which was prepared by coupling several small fragments to tetraysine while attached to a solid support, showed an effective concentration dependence 2.7 times that of alamethicin. Other oligomeric pores are in preparation using aza-crown ether scaffolds. (JS)
Glycoproteomic Analysis of the Secretome of Human Endothelial Cells (2013)

Previous proteomics studies have partially unraveled the complexity of endothelial protein secretion but have not investigated glycosylation, a key modification of secreted and membrane proteins for cell communication. In this study, human umbilical vein endothelial cells were kept in serum-free medium before activation by phorbol-12-myristate-13 acetate, a commonly used secretagogue that induces exocytosis of endothelial vesicles. In addition to 123 secreted proteins, the secretome was particularly rich in membrane proteins. Glycopeptides were enriched by zwitterionic hydrophilic interaction liquid chromatography resins and were either treated with PNGase F and H2(18)O or directly analyzed using a recently developed workflow combining higher-energy C-trap dissociation (HCD) with electron-transfer dissociation (ETD) for a hybrid linear ion trap-orbitrap mass spectrometer. After deglycosylation with PNGase F in the presence of H2(18)O, 123 unique peptides displayed (18)O-deamidation of asparagine, corresponding to 86 proteins with a total of 121 glycosylation sites. Direct glycopeptide analysis via HCD-ETD identified 131 glycopeptides from 59 proteins and 118 glycosylation sites, of which 41 were known, 51 were predicted, and 26 were novel. Two methods were compared: alternating HCD-ETD and HCD-product-dependent ETD. The former detected predominantly high-intensity, multiply charged glycopeptides, whereas the latter preferentially selected precursors with complex/hybrid glycans for fragmentation. Validation was performed by means of glycoprotein enrichment and analysis of the input, the flow-through, and the bound fraction. This study represents the most comprehensive characterization of endothelial protein secretion to date and demonstrates the potential of new HCD-ETD workflows for determining the glycosylation status of complex biological samples.
Photochemical Property of a Myoglobin–CdTe Quantum Dot Conjugate Formed by Supramolecular Host–Guest Interactions (2014)

Abstract A myoglobin–cadmium telluride quantum dot (QD) conjugate was constructed via supramolecular host–guest interactions between a cyclodextrin molecule attached on QD and an adamantyl group covalently linked to the heme. Irradiation of the supramolecular myoglobin–cadmium telluride conjugate generated a photoreduced ferrous myoglobin species via an electron transfer from the photoexcited quantum dot, leading to the formation of CO-bound myoglobin under a CO atmosphere.
Model-Centric Volumetric Point Cloud Attributes (2021)

Point clouds have recently gained interest, especially for real-time applications and for 3D-scanned material, such as is used in autonomous driving, architecture, and engineering, to model real estate for renovation or display. Point clouds are associated with geometry information and attributes such as color. Be the color unique or direction-dependent (in the case of plenoptic point clouds), it reflects the colors observed by cameras displaced around the object. Hence, not only are the viewing references assumed, but the illumination spectrum and illumination geometry is also implicit. We propose a model-centric description of the 3D object, that is independent of the illumination and of the position of the cameras. We want to be able to describe the objects themselves such that, at a later stage, the rendering of the model may decide where to place illumination, from which it may calculate the image viewed by a given camera. We want to be able to describe transparent or translucid objects, mirrors, fishbowls, fog and smoke. Volumetric clouds may allow us to describe the air, however ``empty'', and introduce air particles, in a manner independent of the viewer position. For that, we rely on some eletromagnetic properties to arrive at seven attributes per voxel that would describe the material and its color or transparency. Three attributes are for the transmissivity of each color, three are for the attenuation of each color, and another attribute is for diffuseness. These attributes give information about the object to the renderer, with whom lies the decision on how to render and depict each object.
The angiopoietin receptor Tie2 is atheroprotective in arterial endothelium (2023)

Leukocytes and resident cells in the arterial wall contribute to atherosclerosis, especially at sites of disturbed blood flow. Expression of endothelial Tie1 receptor tyrosine kinase is enhanced at these sites, and attenuation of its expression reduces atherosclerotic burden and decreases inflammation. However, Tie2 tyrosine kinase function in atherosclerosis is unknown. Here we provide genetic evidence from humans and from an atherosclerotic mouse model to show that TIE2 is associated with protection from coronary artery disease. We show that deletion of <i>Tie2</i>, or both <i>Tie2</i> and <i>Tie1</i>, in the arterial endothelium promotes atherosclerosis by increasing Foxo1 nuclear localization, endothelial adhesion molecule expression and accumulation of immune cells. We also show that Tie2 is expressed in a subset of aortic fibroblasts, and its silencing in these cells increases expression of inflammation-related genes. Our findings indicate that unlike Tie1, the Tie2 receptor functions as the dominant endothelial angiopoietin receptor that protects from atherosclerosis.
Recombinant expression, biochemical characterization and biological activities of the human MGSA/gro protein (1990)

Melanoma growth stimulatory activity (MGSA) is a mitogenic protein secreted by Hs294T melanoma cells that corresponds to the polypeptide encoded by the human gro gene. The MGSA/gro cDNA has been expressed in mammalian cells and the secreted recombinant factor has been purified. Biochemical and biological characterization shows that the recombinant protein is identical with the natural protein and is devoid of posttranslational glycosylation, sulfation, and phosphorylation. The two C-terminal amino acids are proteolytically removed from the mature recombinant MGSA, indicating a length of 71 instead of the predicted 73 amino acids. The recombinant MGSA is mitogenically active on the Hs294T melanoma cells. The purified MGSA competes with interleukin 8 for binding to neutrophil receptors and exhibits neutrophil chemotactic activity equivalent to that of interleukin 8.
Purification of aqueous cellulose ethers (1990)

Manufacture of cellulose ethers usually involves high amounts of salt by-products. For application of the product, salt must be removed. In this work, we have studied the injection of high-pressure CO{sub 2} into an aqueous polymer-salt solution; we find that upon addition of isopropanol in addition to CO{sub 2}, the solution separates into two phases. One phase is rich in polymer and water, and the other phase contains mostly isopropanol, water and CO{sub 2}. The salt distributes between the two phases, thereby offering interesting possibilities for development of a new purification process for water-soluble polymers. This work presents experimental phase-equilibrium data for hydroxyethyl cellulose and sodium carboxymethyl cellulose with sodium acetate and potassium sulfate, respectively, in the region 40{degree}C and 30 to 80 bar. Based on these data, we suggest a process for the manufacture and purification of water-soluble cellulose ethers. 15 refs., 14 figs., 9 tabs.
Targeting of phosphatidylserine by monoclonal antibodies augments the activity of paclitaxel and anti-PD1/PD-L1 therapy in the murine breast model E0771 (2015)

Meeting abstracts The phospholipid lipid phosphatidylserine (PS) normally resides in the inner plasma membrane leaflet in most mammalian cells, including tumor and tumor associated vascular cells. Inducers of cellular stress, such as hypoxia and oxygen radicals encountered in tumors, and treatments
High-frequency (95 GHz) electron paramagnetic resonance study of the photoinduced charge transfer in conjugated polymer-fullerene composites (2001)

Light-induced electron paramagnetic resonance (LEPR) measurements are reported in composites of poly(2-methoxy-5-(3-,7-dimethyloctyloxy)-1,4-phenylenevinylene) (MDMO-PPV) and ${[6,6]\ensuremath{-}\mathrm{p}\mathrm{h}\mathrm{e}\mathrm{n}\mathrm{y}\mathrm{l}\ensuremath{-}\mathrm{C}}_{61}\ensuremath{-}\mathrm{b}\mathrm{u}\mathrm{t}\mathrm{y}\mathrm{r}\mathrm{i}\mathrm{c}$ acid methyl ester (PCBM), a soluble derivative of ${\mathrm{C}}_{60}.$ Under illumination of the sample, two paramagnetic species are formed due to photoinduced charge transfer between conjugated polymer and fullerene. One is the positive polaron ${\mathrm{P}}^{+}$ on the polymer backbone and the other is the radical anion on the methanofullerene. Using high-frequency (95 GHz) LEPR it was possible to separate these two contributions to the spectrum on the basis of their g factors, and moreover to resolve the g anisotropy for both radicals. The positive polaron on the conjugated polymer chain possesses axial symmetry with g values ${g}_{\ensuremath{\Vert}}=2.0034(1)$ and ${g}_{\ensuremath{\perp}}=2.0024(1).$ EPR on low doped polymer gave extra proof for the assignment to the positive polaron. The negatively charged methanofullerene has a lower, rhombic symmetry with ${g}_{x}=2.0003(1),$ ${g}_{y}=2.0001(1),$ and ${g}_{z}=1.9982(1).$ Different spin-lattice relaxation of both species gives rise to a rapid passage effect for the positive polaron spectrum.
Supramolecular acid/base catalysis via multiple hydrogen bonding interaction (2002)

2-Ureidoisocytosine bearing a phosphonium ion functionality self-assembles in an anti-parallel manner, to exhibit a cooperative acid/base catalysis for the ring-opening reaction of an epoxide.
Growth-Inhibited Nucleation of Ni Nanoparticles On TiN Substrates (2013)

Abstract not Available.
Design and Performance of 1×8 Core Selective Switch Supporting 15 Cores Per Port Using Bundle of Three 5-Core Fibers (2022)

A core-selective switch (CSS) is a key building block for a port modular spatial cross-connect where an optical signal launched into any core in the input multi-core fiber (MCF) can be switched to a core that has the same core identifier of any output MCF. One way to increase the core count in a CSS is to use several MCFs as a bundle and collimate/demultiplex beams emitted from it all at once using a single microlens. In this paper, we report a 1×8 CSS prototype based on a bundle of three 5-core fibers (5-CFs), which supports 15 cores per port, demonstrating the feasibility of a high core count CSS through MCF bundling. The CSS prototype exhibits insertion loss of less than 4.8 dB, and polarization dependent loss of less than 0.5 dB over an ultrawide wavelength range of 1480 nm to 1630 nm. The inter-core crosstalk (XT) characteristics as a function of the wavelength are thoroughly investigated. We show that the intra 5-CF XT imparted to the center core from the other four outer cores in the same 5-CF is less than −52 dB and the inter 5-CF XT from cores in the other 5-CFs in the same bundle is less than -64 dB, which are sufficiently low values for practical networks. No OSNR penalty in the 100-Gb/s spatial channel was observed when ten 100-Gb/s DP-QPSK WDM signals in the C-band were simultaneously routed by the bundled 5-CF 1×8 CSS prototype.
Bleomycin hydrolase: molecular cloning, sequencing, and biochemical studies reveal membership in the cysteine proteinase family (1989)

Bleomycin (BLM) hydrolase catalyzes the inactivation of the antitumor drug BLM and is believed to protect normal and malignant cells from BLM toxicity. The normal physiological function of BLM hydrolase is not known. We now provide evidence for its membership in the cysteine proteinase family. BLM hydrolase was purified to homogeneity from rabbit lungs, and a partial amino acid sequence was determined from a tryptic digest peptide. On the basis of this sequence a 36-mer oligonucleotide was synthesized. The 36-mer oligonucleotide probe hybridized to a single mRNA species of 2.5 kb from several species and was used to isolate an 832-bp cDNA insert from a lambda gt11 rabbit liver cDNA library. This insert encoded the tryptic digest peptide previously identified in rabbit lung BLM hydrolase by amino acid sequencing. Analysis of the predicted amino acid sequence coded by the 832-bp BLM hydrolase cDNA fragment indicated no significant homology with any currently known proteins except for a 15 amino acid portion, which displayed remarkable homology with the active site of cysteine proteinases. Within this active-site region, 10 of the amino acid residues of papain and 9 of aleurain, cathepsin H, and cathepsin L were identical with those of rabbit liver BLM hydrolase. The catalytic cysteine of thiol proteinases was also conserved in BLM hydrolase, and cysteine proteinase specific inhibitors, such as E-64, were found to be potent inhibitors of BLM hydrolase activity. Furthermore, bleomycin hydrolase exhibited cathepsin H like enzymatic activity. Bleomycin hydrolase had, however, no significant cathepsin B or L activities.(ABSTRACT TRUNCATED AT 250 WORDS)
CCL28 Is Involved in Mucosal IgA Responses, Olfaction, and Resistance to Enteric Infections (2019)

CCL28 is a mucosal chemokine that has been involved in various responses, including IgA production. We have analyzed its production in human tissues using a comprehensive microarray database. Its highest expression is in the salivary gland, indicating that it is an important component of saliva. It is also expressed in the trachea, bronchus, and in the mammary gland upon onset of lactation. We have also characterized a Ccl28<sup>-/-</sup> mouse that exhibits very low IgA levels in milk, and the IgA levels in feces are also reduced. These observations confirm a role for the CCL28/CCR10 chemokine axis in the recruitment of IgA plasmablasts to the lactating mammary gland. CCL28 is also expressed in the vomeronasal organ. We also detected olfactory defects (anosmia) in a Ccl28<sup>-/-</sup> mouse suggesting that CCL28 is involved in the function/development of olfaction. Importantly, Ccl28<sup>-/-</sup> mice are highly susceptible to Salmonella enterica serovar Typhimurium in an acute model of infection, indicating that CCL28 plays a major role in innate immunity against Salmonella in the gut. Finally, microbiome studies revealed modest differences in the gut microbiota between Ccl28<sup>-/-</sup> mice and their cohoused wild-type littermates. The latter observation suggests that under homeostatic conditions, CCL28 plays a limited role in shaping the gut microbiome.
Load-dependent electrical contact resistance of carbon nanotube-reinforced metal matrix composites (2022)

The reliability of contacting systems is determined, among other factors, by its electrical contact resistance (ECR) development. ECR in closed state must be as low as possible, to avoid energy losses, overheating and to ensure the proper circuit opening afterwards. Furthermore, to improve the electrode's performance, there are key characteristics that should be optimized such as its mechanical and thermal stability. This study analyses the behavior of the ECR as a function of the applied normal load of carbon nanotube (CNT)-reinforced Ni composites with different concentrations. The first test consisted of performing a DC current sweep from 30 µA to 1 A at a constant mechanical load (4 N) and contrast the behavior to a pure metal sample. In all the CNT-containing samples, the contact resistance was below that of the reference sample, achieving a reduction of up to 80%. The second test dealt with the load-dependent behavior of the contact resistance. The normal load was varied from 0.25 to 10 N and the circuit DC current was 100 mA. In this case, the composites showed lower contact resistance for a wide load range, except at the highest load, where the dominant mechanisms may change to a pure metal-metal contact.
Abnormal Expression of Secretory Component in Term Newborns with Bowel Perforation—A Report of Two Cases (1986)

Summary: Expression of secretory component (SC) in intestinal resection specimens from two term newborns with bowel perforation due to necrotizing enterocolitis (NEC) or an NEC-like syndrome was determined and compared with the SC immunoreactivity in a panel of intestinal tissues from fetuses at different ages of gestation. Expression of SC was absent in one patient, whereas in the other patient, SC immunoreactivity was observed in the basal cell part of colonic epithelium only. SC was normally expressed in a follow-up biopsy on one of these patients. In contrast, SC was present in the apical plasma membrane and brush border of colonic epithelium from 32 weeks of gestation onwards in fetuses without demonstrable intestinal disease. From these findings we conclude that the expression of SC was impaired in our patients, thus possibly playing a role in the development of the bowel perforation in these full-term newborns with an NEC-like syndrome or resulting from its pathogenesis.
Correction to Multivariable Sensors for Ubiquitous Monitoring of Gases in the Era of Internet of Things and Industrial Internet (2016)

ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionNEXTORIGINAL ARTICLEThis notice is a correctionCorrection to Multivariable Sensors for Ubiquitous Monitoring of Gases in the Era of Internet of Things and Industrial InternetRadislav A. Potyrailo*Cite this: Chem. Rev. 2016, 116, 23, 14918Publication Date (Web):November 15, 2016Publication History Published online15 November 2016Published inissue 14 December 2016https://pubs.acs.org/doi/10.1021/acs.chemrev.6b00741https://doi.org/10.1021/acs.chemrev.6b00741correctionACS PublicationsCopyright © 2016 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views1275Altmetric-Citations3LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (165 KB) Get e-Alertsclose Get e-Alerts
Imprinted antibody responses against SARS-CoV-2 Omicron sublineages (2022)

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron sublineages carry distinct spike mutations resulting in escape from antibodies induced by previous infection or vaccination. We show that hybrid immunity or vaccine boosters elicit plasma-neutralizing antibodies against Omicron BA.1, BA.2, BA.2.12.1, and BA.4/5, and that breakthrough infections, but not vaccination alone, induce neutralizing antibodies in the nasal mucosa. Consistent with immunological imprinting, most antibodies derived from memory B cells or plasma cells of Omicron breakthrough cases cross-react with the Wuhan-Hu-1, BA.1, BA.2, and BA.4/5 receptor-binding domains, whereas Omicron primary infections elicit B cells of narrow specificity up to 6 months after infection. Although most clinical antibodies have reduced neutralization of Omicron, we identified an ultrapotent pan-variant-neutralizing antibody that is a strong candidate for clinical development.
Characterization of Hydrocarbon Emissions from Gasoline Direct-Injection Compression Ignition Engine Operating on a Higher Reactivity Gasoline Fuel (2017)

&lt;div class="section abstract"&gt;&lt;div class="htmlview paragraph"&gt;Low temperature combustion engine technologies are being investigated for high efficiency and low emissions. However, such engine technologies often produce higher engine-out hydrocarbon (HC) and carbon monoxide (CO) emissions, and their operating range is limited by the fuel properties. In this study, two different fuels, a US market gasoline containing 10% ethanol (RON 92 E10) and a higher reactivity gasoline (RON 80 E0), were compared on Delphi’s second generation Gasoline Direct-Injection Compression Ignition (Gen 2.0 GDCI) multi-cylinder engine. The engine was evaluated at three operating points ranging from a light load condition (800 rpm/2 bar IMEP&lt;sub&gt;g&lt;/sub&gt;) to medium load conditions (1500 rpm/6 bar and 2000 rpm/10 bar IMEP&lt;sub&gt;g&lt;/sub&gt;). The engine was equipped with two oxidation catalysts, between which was located the exhaust gas recirculation (EGR) inlet. Samples were taken at engine-out, between the catalysts, and at tailpipe locations. In addition, part of the raw exhaust was diluted and sampled for HC speciation. Canisters and sorbent membranes were used to collect volatile HCs and semi-volatile HCs, respectively. Di-nitrophenyl hydrazine (DNPH) cartridges were also used for collecting oxygenated species. Results showed overall lower HC emissions with the RON 80 E0 fuel compared to the RON 92 E10 fuel. For both fuels, the percentage of aromatic HCs was higher in the exhaust than in the fuels themselves. High aldehyde and ketone emissions were observed for both fuels. Detailed exhaust HC speciation can provide valuable information for modeling GDCI processes and eventually for determining the optimum combustion control methodologies to increase combustion efficiency and lower engine-out CO and HC emissions. In addition, HC speciation is useful for the development of robust emission control systems.&lt;/div&gt;&lt;/div&gt;
Double Unit Umbilical Cord Blood Transplant for Adults with Acute Leukemia and Myelodysplastic Syndrome Results in Comparable Outcome As Matched Sibling or Unrelated Donor Transplant Only after Myeloablative Conditioning but Not Reduced Intensity Conditio (2014)

The infusion of 2 partially HLA-matched umbilical cord blood (UCB) is a potential strategy for extending UCB transplantation to more patients. The risk and benefits of double UCB transplantation (dUCBT) relative to those observed after transplantation with related and unrelated adult donors remains to be determined.
Supplementary materials and methods from <i>S</i>-nitrosylation of the Mitochondrial Chaperone TRAP1 Sensitizes Hepatocellular Carcinoma Cells to Inhibitors of Succinate Dehydrogenase (2023)

&lt;p&gt;Supplementary materials and methods&lt;/p&gt;