820 TEMPORAL CHANGES IN NEUROTROPHIC FACTORS AND NEURITE GROWTH FOLLOWING CAVERNOUS NERVE INJURY (2013)

You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Basic Research (I)1 Apr 2013820 TEMPORAL CHANGES IN NEUROTROPHIC FACTORS AND NEURITE GROWTH FOLLOWING CAVERNOUS NERVE INJURY Johanna L. Hannan, Bernard L. Stopak, Maarten Albersen, Xiaopu Liu, Arthur L. Burnett, Frank van der Aa, Petter Hedlund, and Trinity J. Bivalacqua Johanna L. HannanJohanna L. Hannan Baltimore, MD More articles by this author , Bernard L. StopakBernard L. Stopak Baltimore, MD More articles by this author , Maarten AlbersenMaarten Albersen Leuven, Belgium More articles by this author , Xiaopu LiuXiaopu Liu Baltimore, MD More articles by this author , Arthur L. BurnettArthur L. Burnett Baltimore, MD More articles by this author , Frank van der AaFrank van der Aa Leuven, Belgium More articles by this author , Petter HedlundPetter Hedlund Lund, Sweden More articles by this author , and Trinity J. BivalacquaTrinity J. Bivalacqua Baltimore, MD More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.386AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Surgical treatments for prostate, bladder and colorectal cancers often lead to neuronal damage and erectile dysfunction (ED). Despite nerve-sparing techniques and treatments that protect cavernous smooth muscle, ED prevails and protecting/preventing neurodegeneration may be of greater importance. The aim of this study was to characterize the changes in neurotrophic factors and neurite growth from the major pelvic ganglion (MPG) following cavernous nerve injury. METHODS Male Sprague-Dawley rats underwent sham or bilateral cavernous injury (BCNI) and MPGs were isolated 2, 7, 14, 30 and 60 days following injury (n=5/group). MPG gene expression analysis by qtPCR was performed for glial cell line-derived neurotrophic factor (GDNF), nerve growth factor (NGF), neurturin (NRTN), neurotrphin-3 (NT3) and neurotrophin-4 (NT4). Additional rats were injured and MPGs were removed 24h, 48h, 3 and 7 days following BCNI (n=3/group). MPGs were cultured in reduced growth factor matrigel with vascular endothelial growth factor (VEGF, 25ng/ml) for 48h and neurite growth was measured. RESULTS GDNF was significantly increased in MPGs at all time points following BCNI (p<0.05). There was a significant decrease in NGF, NT3 and NT4 in all injured MPGs (p<0.05). No significant changes in NRTN were evident between sham and BCNI MPGs. MPG neurite growth from 24h and 48h BCNI was significantly higher than sham (508±19ím; 497±24ím; 373±23ím, respectively, p<0.05). Neurite growth from 3 and 7 day BCNI MPGs was not different from shams (328±24ím; 387±32ím; 373±23ím, respectively, p>0.05). CONCLUSIONS Changes in neurotrophic factors GDNF, NT3 NT4 and NGF contribute to neuromodulation and enhanced neurite outgrowth following BCNI. Increased neurite outgrowth is seen in MPGs from 24h and 48h BCNI rats and should be used as a tool to assess the impact of pharmacological interventions. Further studies examining the roles of neurotrophic factors in modulating signaling pathways may provide therapeutic avenues for neurogenic-mediated ED. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e336-e337 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Johanna L. Hannan Baltimore, MD More articles by this author Bernard L. Stopak Baltimore, MD More articles by this author Maarten Albersen Leuven, Belgium More articles by this author Xiaopu Liu Baltimore, MD More articles by this author Arthur L. Burnett Baltimore, MD More articles by this author Frank van der Aa Leuven, Belgium More articles by this author Petter Hedlund Lund, Sweden More articles by this author Trinity J. Bivalacqua Baltimore, MD More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...