gebhart/patrepeval huggingface dataset:
Characterization of immunologic properties of a second HLA-A2 epitope from a granule protease in CML patients and HLA-A2 transgenic mice (2011)

The serine proteases, neutrophil elastase (HNE) and proteinase 3 (PR3), are aberrantly expressed in human myeloid leukemias. T-cell responses to these proteins have been correlated with remission in patients with chronic myeloid leukemia (CML). Human PR3/HNE-specific CD8(+) T cells predominantly recognize a nonameric HLA-A2-restricted T-cell epitope called PR1 which is conserved in both Ags. However, CML patients have CD8(+) T cells in peripheral blood recognizing an additional HLA-A2 epitope termed PR2. To assess immunologic properties of these Ags, novel recombinant vaccinia viruses (rVV) expressing PR3 and HNE were evaluated in HLA-A2 transgenic (Tg) mice (HHDII). Immunization of HHDII mice with rVV-PR3 elicited a robust PR3-specific CD8(+) T-cell response dominated by recognition of PR2, with minimal recognition of the PR1 epitope. This result was unexpected, because the PR2 peptide has been reported to bind poorly to HLA. To account for these findings, we proposed that HHDII mice negatively selected PR1-specific T cells because of the presence of this epitope within murine PR3 and HNE, leading to immunodominance of PR2-specific responses. PR2-specific splenocytes are cytotoxic to targets expressing naturally processed PR3, though PR1-specific splenocytes are not. We conclude that PR2 represents a functional T-cell epitope recognized in mice and human leukemia patients. These studies are registered at www.clinicaltrials.gov as NCT00716911.
A robust modeling and design approach for dynamically loaded and digitally linearized Doherty amplifiers (2005)

In this paper, an active load-pull-based large-signal modeling approach suitable for designing and optimizing Doherty amplifiers is proposed. The optimization of the drive dependant dynamic loads seen by both carrier and peaking amplifier's transistors was carried out using a large-signal load-pull-based behavior model built into CAD software. The latter was also used to optimize the biasing conditions of the peaking and carrier amplifier transistors. The simulation versus measurement results demonstrated the strong ability of the constructed model to predict the nonlinear behavior of 16 watts peak-envelope power designed Doherty amplifier in terms of power added efficiency, AM/AM, and AM/PM distortions. In addition, a complex digital predistortion technique was used to significantly enhance the linearity of the Doherty amplifier and to preserve an ACPR level better than 50 dBc under a WCDMA signal while attaining a power added efficiency of about 44% at the same time.
Production of monovalent antisera by induction of immunological tolerance for capsular typing of Streptococcus pneumoniae (1992)

Hyperimmune and high-titered polyclonal pneumococcal antisera, specific for cross-reactive types within groups, were produced in adult rabbits. Purified capsular polysaccharide was injected intravenously into adult rabbits. One week later, these rabbits were given multiple intravenous injections of formalin-inactivated pneumococci of the cross-reactive type by an established method. Each of the resultant antisera were specific for the cross-reactive type indicating that the previous injection of the polysaccharide had induced epitope-specific tolerance. This method was successful for production of antisera against pneumococcal types 6A, 6B, 9N, 9V, 19F and 19A. Polyclonal rabbit pneumococcal antisera have some advantages over murine monoclonal antibodies for serologic studies and this method should be applicable for producing type-specific antibodies to cross-reactive polysaccharides of clinical interest. Further, this method is simpler and generally produces higher titered monovalent (factor) reagents than absorbed antisera.
Tabletop Femtosecond VUV Photoionization and PEPICO Detection of Microreactor Pyrolysis Products (2017)

We report the combination of tabletop vacuum ultraviolet photoionization with photoion-photoelectron coincidence spectroscopy for sensitive, isomer-specific detection of nascent products from a pyrolysis microreactor. Results on several molecules demonstrate two essential capabilities that are very straightforward to implement: the ability to differentiate isomers and the ability to distinguish thermal products from dissociative ionization. Here, vacuum ultraviolet light is derived from a commercial tabletop femtosecond laser system, allowing data to be collected at 10 kHz; this high repetition rate is critical for coincidence techniques. The photoion-photoelectron coincidence spectrometer uses the momentum of the ion to identify dissociative ionization events and coincidence techniques to provide a photoelectron spectrum specific to each mass, which is used to distinguish different isomers. We have used this spectrometer to detect the pyrolysis products that result from the thermal cracking of acetaldehyde, cyclohexene, and 2-butanol. The photoion-photoelectron spectrometer can detect and identify organic radicals and reactive intermediates that result from pyrolysis. Direct comparison of laboratory and synchrotron data illustrates the advantages and potential of this approach.
ChemInform Abstract: Fluorinated Building Blocks. The Discovery of a Stable Difluoroallenyl Indium and the Synthesis of gem‐Difluoroallenyl and ‐propargyl Synthons in Aqueous Media. (2001)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Relating functional changes during hand movement to clinical parameters in patients with multiple sclerosis in a multi‐centre fMRI study (2008)

We performed a prospective multi-centre study using functional magnetic resonance imaging (fMRI) to better characterize the relationships between clinical expression and brain function in patients with multiple sclerosis (MS) at eight European sites (56 MS patients and 60 age-matched, healthy controls). Patients showed greater task-related activation bilaterally in brain regions including the pre- and post-central, inferior and superior frontal, cingulate and superior temporal gyri and insula (P < 0.05, all statistics corrected for multiple comparisons). Both patients and healthy controls showed greater brain activation with increasing age in the ipsilateral pre-central and inferior frontal gyri (P < 0.05). Patients, but not controls, showed greater brain activation in the anterior cingulate gyrus and the bilateral ventral striatum (P < 0.05) with less hand dexterity. An interaction between functional activation changes in MS and age was found. This large fMRI study over a broadly selected MS patient population confirms that movement for patients demands significantly greater cognitive 'resource allocation' and suggests age-related differences in brain responses to the disease. These observations add to evidence that brain functional responses (including potentially adaptive brain plasticity) contribute to modulation of clinical expression of MS pathology and demonstrate the feasibility of a multi-site functional MRI study of MS.
Immunobinding Assay for the Speciation of Avian Mycoplasmas Adapted for Use with a 96-Well Filtration Manifold (1991)

An immunobinding assay capable of distinguishing among Mycoplasma synoviae, M. gallisepticum, M. gallopavonis, and M. meleagridis was developed. A low-protein-binding membrane filter served as the solid support, and a 96-well microsample filtration manifold was used. The assay detected 50 ng of mycoplasmal protein, or approximately 3 x 10(4) colony-forming units, from pure or mixed cultures. No cross-reactions were observed among the mycoplasma species tested. The assay was inexpensive, easy to interpret, and required approximately 3 hr to perform. The 96-well format allowed screening of numerous cultures and use of several antisera in a single assay.
Highly Efficient Self-Referencing of a 1-GHz Diode-Pumped Solid-State Laser Using a Silicon Nitride Chip (2015)

We present the first carrier-envelope-offset (CEO) frequency detection based on multi-octave-spanning supercontinuum generation in a CMOS-compatible Si3N4 waveguide. Strong CEO-beat-signals (&gt;30 dB) of a gigahertz-Yb:CALGO-laser were obtained with only 36 pJ of coupled pulse energy.
Abstract 15230: MiR-30e Reduces Osteogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cells by Repressing IGF2 Expression (2013)

Objective: In atherosclerosis, activation of an osteogenic transcriptional program in de-differentiated mesenchymal-like smooth muscle cells contributes substantially to the initiation of aortic calcification. MiR-30e was recently reported as the most downregulated miR in the aortas of atherogenic APOE-/- mice. To assess a causal link of deficient miR-30e, we tested the hypothesis that overexpression of miR-30e would inhibit osteogenic differentiation of bone marrow derived mesenchymal stem cells (MSCs).

Method and Results: Using qPCR and Elisa respectively, we confirmed that the expression of the mRNA and protein for the osteogenic marker osteopontin (OPN) was upregulated (p<.01) in aortas of 6 mo old APOE-/- mice (n=9) relative to wild type controls (n=4). In MSCs transduced with lentivirus for miR-30e or a scrambled (scr) control, microarrays and qPCR showed repression (p<.05) of an osteogenic profile including Opn, Igf2, Runx2, Bmp4, Dpt, and Dcn genes. At 2 wk osteogenic differentiation, this osteogenic gene expression panel was dramatically enhanced in the MSCs but reduced (p<.05) by miR-30e, with a decrease (p<.05) in OPN protein levels. Secreted IGF2 protein was decreased in the MSCs over-expressing miR-30e at 1wk osteogenic differentiation. Electron microscopy after 2wk osteogenic differentiation showed a marked morphological difference in the miR-30e stables versus controls. MiR-30e oligos with 2’Fluoro-modification also significantly decreased the above osteogenic panel together with Alp1 transcripts, in a dose-dependent manner. MiR-30e reduced (p<.01) proliferation of MSCs at Days 2, 4, and 5 post plating. Inhibiting miR-30e in MSCs using antimiR-30e oligos followed by microarrays and qPCR showed over-expression of Igf2 transcripts suggesting that Igf2 could be a primary target of miR-30e-driven osteogenic repression. Double luciferase assay showed binding of miR-30e to the 3’UTR of Igf2 (p=.00001) and positive control Runx2 (p=.01) but not Opn.

Conclusion: miR-30e represses the osteogenic potential of MSCs by targeting IGF2, and its dowregulation may be a proximate cause of the initiation of atherosclerosis. The miR-30e pathway represents a potentially powerful and important clinical target in vascular diseases.
Gray Zone and Threshold Current Measurements of the Josephson Balanced Comparator (2021)

The Josephson balanced comparator, a decision-making circuit comprising two Josephson junctions (JJs) connected in series from a clocking perspective and in parallel for the current to be measured, is ubiquitous in single flux quantum (SFQ) logic. Its noise properties are crucial for the performance of logic devices. The characteristics of the balanced comparator can also be used to monitor fab process and design implementation as an indicator for excess noise, overheating, linearity, dynamic effects, etc. We designed several test structures to measure gray zone of various comparators fabricated in different nodes of process at MIT-LL. We used digital circuitry to measure comparator characteristics at low frequencies and an analog testbed to perform high frequency characterization. Experimental results for gray zone of comparators designed for fabrication nodes with different current densities, sheet resistances, critical damping are presented and studied as a function of clock frequency.
Supplementary Table 1 from Pharmacologic Unmasking of Epigenetically Silenced Genes in Breast Cancer (2023)

Supplementary Table 1 from Pharmacologic Unmasking of Epigenetically Silenced Genes in Breast Cancer
Small Circular Polarized Antenna with Spiral Elements Suppressing Back-lobe Radiation (2022)

In this paper, we propose a method to decrease back-lobe radiation of the small Circular Polarized (CP) antenna. The proposed CP antenna is composed of four antenna elements arranged in a rotational symmetry at 90 degrees on the ground, which are excited with the same amplitude and 90 degrees phase difference. Each antenna element is formed by a spiral antenna branched from the near the middle of an inverted-L antenna. From the result of simulation, backward radiation of the proposed CP antenna can be decreased significantly by adding the spiral elements.
Quantifying solar cell cracks in photovoltaic modules by electroluminescence imaging (2015)

This article proposes a method for quantifying the percentage of partially and totally disconnected solar cell cracks by analyzing electroluminescence images of the photovoltaic module taken under high- and low-current forward bias. The method is based on the analysis of the module's electroluminescence intensity distribution, applied at module and cell level. These concepts are demonstrated on a crystalline silicon photovoltaic module that was subjected to several rounds of mechanical loading and humidity-freeze cycling, causing increasing levels of solar cell cracks. The proposed method can be used as a diagnostic tool to rate cell damage or quality of modules after transportation. Moreover, the method can be automated and used in quality control for module manufacturers, installers, or as a diagnostic tool by plant operators and diagnostic service providers.
Analysis of bronchoalveolar immune cells and cytokines induced by immunization with different respiratory syncytial virus vaccines (P4395) (2013)

Abstract Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract infection and deaths in infants. Since the failure of formalin-inactivated RSV (FI-RSV) due to vaccine-enhanced pulmonary eosinophilia and pneumonia during 1960s, there is no licensed vaccine against RSV. In this study, we analyzed immune cell phenotypes and cytokines in bronchoalveolar lavage fluids (BALF) from mice immunized with FI-RSV, live RSV, or F plasmid and virus-like particles (VLP) containing RSV F and G glycoproteins (FFG VLP) at day 5 post RSV challenge. FI-RSV immunized mice showed severe eosinophilia, high levels of CD11b+ cells as well as IFN-gamma and IL-4 secreting CD4 T cells locally and systemically. Mice pre-immunized with live RSV showed no severe pulmonary eosinophilia, similar levels of IFN-gamma secreting CD4 and CD8 T cells, and low levels of IL-4 secreting cells. Mice immunized with FFG VLPs displayed no eosinophilia, relatively a low ratio of CD4/CD8 T cells secreting IFN-gamma, and no systemic cytokines. All 3 types of RSV vaccines were effective in clearing lung viral loads. Taken together, these results suggest no correlation between lung RSV clearance and inflammatory pulmonary disease, and that FFG VLP can be a promising RSV vaccine candidate conferring protection without lung disease.
P‐059: Voxel‐based analysis of [11C]PIB uptake in Alzheimer's disease and mild cognitive impairment (2007)

Beta-amyloid plaques are the hallmark of Alzheimer's disease (AD). Mild cognitive impairment is a transitional stage between normal aging and early AD. [11C]PIB has been successfully used for studies of amyloid deposition in the brain of AD patients in vivo. Previous studies with [11C]PIB PET showed that some MCI subjects showed a high amyloid deposition as AD patients, while some MCI subjects did not show any amyloid deposition in the brain. However, data analysis of MCI patients has been based on region-of-interest (ROI) analysis of selected brain regions. To investigate detailed distribution of amyloid deposition in MCI subjects by voxel-based analysis of [11C]PIB PET. The subjects consisted of 12 patients with AD (4M and 8F, 69 ± 8 yrs, MMSE score 17 ± 5), 8 MIC subjects (6M and 2F, 76 ± 4 yrs, MMSE score 27 ± 2), and 15 healthy subjects (5M and10F, 66 ± 12 yrs, MMSE score 28 ± 2). [11C]PIB -PET acquisition was a dynamic sequence of 19 PET scans for 90 min. Spatial normalization of PET images were performed using a specific [11C]PIB template, which was created from 24 [11C]PIB scans. The Logan graphical analysis using arterial input function was employed to calculate distribution volume (DV). The target tissue to the cerebellum DV ratio (DVR) was calculated as an index of amyloid deposition in each voxel. Two sample t-tests were performed between 12 AD patients vs 15 healthy subjects, and 8 MCI subjects vs 15 healthy subjects. The results suggest that distribution of amyloid deposition in the brain does not change when MCI subjects progress to AD.
Non invasive and real time evaluation of mice aortic stiffness by ultrafast ultrasound imaging: a new tool for evaluation of preclinical vascular disease models (2013)

Background: Mice models of arterial disease are critical for a better understanding of vascular diseases. Pulse wave velocity (PWV) is widely used as an index of arterial stiffness. Indeed, its evaluation has been reported through invasive measurement by the ratio of distance between 2 arterial cannula tips divided by transit time or non-invasively by MRI which requires a long acquisition time. Our goal was to evaluate the capacity of a new ultrasound ultrafast imaging technique, at high frame rate (up to 5 kHz) to determine non-invasively, in real-time, the abdominal aortic PWV at different levels of blood pressure. Methods: Littermates BalbC wild type mice (n=6) were used with comparable ages (303±95days) and weights (27±3g). Invasive arterial blood pressure (BP) catheter allowed to measure acute pressor responses to increasing doses of phenylephrine (PE) (0.01–700 μg/kg) infused via a venous catheter in anesthetized mice with pentobarbital sodique (60mg/kg/ip). The abdominal aorta was imaged using a conventional linear ultrasonic probe (15MHz, 256 elements) connected to a unique ultrafast scanner (SuperSonic Imagine, France) acquiring at 5400 frames/s which allows to visualize aortic PWV. Tissue velocities of the arterial walls were computed off line and analyzed by speckle tracking technique to provide the pulse wave velocity. Results are mean±SD. Results: We identified 2 PWVs one at the ECG-peak-R-wave, at the time of aortic diastolic blood pressure (PWV-DBP) and one at the ECG-end-T-wave at the time of aortic systolic blood pressure (PWV-SBP). Heart Rate was 415±50bpm. These 2 PWVs were forward waves running along the arterial wall. The mean difference between PWV-DBP and PWV-SBP was 2.3±1.4 m/s. A linear relationship between PWV and BP was observed for the 2 PWVs ranging from 2.5 and 10.0 m/s for a pressure range of 55 to 265 mmHg. Interestingly this linear relation of aortic BP was similar in diastole and in systole with respective slope of 0.037±0.008m/s/mmHg and 0.035±0.010m/s/mmHg, with a good fit: r2= 0.90 and 0.83 respectively. Conclusion: We demonstrated the capability of new ultrafast ultrasound scanner to visualize and calculate non invasively in real time mice aortic PWV at different times of cardiac cycle despite very high heart rate in mice. Moreover, this new technology allows to better characterize the aortic wall biomechanical properties by evaluation of the PWV-BP slope. We hope that this breakthrough will allow in the near future to better evaluate aorta biomechanical properties in preclinical models of vascular disease.
Early Syphilitic Hepatitis After Renal Transplantation (1988)

Journal Article Early Syphilitic Hepatitis After Renal Transplantation Get access Philip C. Johnson, Philip C. Johnson Departments of Internal Medicine, Infectious Diseases and Clinical Microbiology, Pathology, and Surgery, The University of Texas Health Science Center at Houston, Houston, Texas Search for other works by this author on: Oxford Academic PubMed Google Scholar Steven J. Norris, Steven J. Norris Departments of Internal Medicine, Infectious Diseases and Clinical Microbiology, Pathology, and Surgery, The University of Texas Health Science Center at Houston, Houston, Texas Search for other works by this author on: Oxford Academic PubMed Google Scholar G. P. G. Miller, G. P. G. Miller Departments of Internal Medicine, Infectious Diseases and Clinical Microbiology, Pathology, and Surgery, The University of Texas Health Science Center at Houston, Houston, Texas Search for other works by this author on: Oxford Academic PubMed Google Scholar Larry D. Scott, Larry D. Scott Departments of Internal Medicine, Infectious Diseases and Clinical Microbiology, Pathology, and Surgery, The University of Texas Health Science Center at Houston, Houston, Texas Search for other works by this author on: Oxford Academic PubMed Google Scholar Stewart Sell, Stewart Sell Departments of Internal Medicine, Infectious Diseases and Clinical Microbiology, Pathology, and Surgery, The University of Texas Health Science Center at Houston, Houston, Texas Search for other works by this author on: Oxford Academic PubMed Google Scholar Barry D. Kahan, Barry D. Kahan Departments of Internal Medicine, Infectious Diseases and Clinical Microbiology, Pathology, and Surgery, The University of Texas Health Science Center at Houston, Houston, Texas Search for other works by this author on: Oxford Academic PubMed Google Scholar Charles T. Van Buren Charles T. Van Buren Departments of Internal Medicine, Infectious Diseases and Clinical Microbiology, Pathology, and Surgery, The University of Texas Health Science Center at Houston, Houston, Texas Search for other works by this author on: Oxford Academic PubMed Google Scholar The Journal of Infectious Diseases, Volume 158, Issue 1, July 1988, Pages 236–238, https://doi.org/10.1093/infdis/158.1.236 Published: 01 July 1988 Article history Received: 25 January 1988 Published: 01 July 1988
Temperature Dependence and Postirradiation Annealing Response of the $1/f$ Noise of 4H-SiC MOSFETs (2013)

The temperature dependence of the low-frequency noise of 4H-silicon carbide (SiC) MOSFETs with nitrided oxides is reported over the temperature range 85-510 K. The 1/f noise decreases significantly with increasing measurement temperature. This decrease in noise results primarily from a decrease in the density of interface traps at increasing temperatures. The 1/f noise is also characterized after total ionizing dose irradiation and postirradiation annealing. No significant change in the 1/f noise is observed after the devices are irradiated to 1 Mrad (SiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> ) and then annealed under bias at elevated temperature. These results show that the 1/f noise in 4H-SiC MOSFETs is dominated by the interaction of channel carriers with slow interface traps at temperatures <; 360 K and with border traps >360 K. This result contrasts with most experience with Si/SiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> -based MOSFETs, and results from the wider bandgap and greater density of slow interface traps in SiC/SiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> -based MOSFETs than in Si/SiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> -based MOSFETs.