gebhart/patrepeval huggingface dataset:
Abstract P238: Regulation Of G Protein-coupled Receptor Kinase 4 Expression By Serum In Breast Cancer And Renal Proximal Tubule Cells (2021)

G protein-coupled receptor kinase 4 (GRK4) is a member of the GRK family which play critical role in regulation of the function of G protein-coupled receptors. Our previous studies have shown that GRK4 not only plays a role in regulating sodium excretion in renal proximal tubule cells but also acts as a stimulator on proliferation of breast cancer cells. Uncontrolled proliferation is a characteristics of cancer cells and GRK4 is upregulated in breast cancer cells. We hypothesized that expression of GRK4 may be regulated differently in cancer and non-cancer cells. To test this hypothesis, expression of GRK4 in response to serum was compared in breast cancer cells and renal proximal tubule cells by Western analysis. In three breast cancer cell lines serum withdrawal caused rapid reduction in the levels of GRK4 which occurred as early as 15 min. GRK4 levels correlated with the concentrations of serum added to the culture media. To determine if growth factors were a critical element for maintaining GRK4 levels in the cells, EGF (10-20 ng/ml) was added to serum free medium for 24 h. There was no increase in GRK4 levels in the cells treated with EGF compared with the serum starvation control. Similarly, serum withdrawal (16 h) led to 40-80% decrease of GRK4 levels in renal proximal tubule cells even in the presence of EFG supplement. Serum feeding for 30 min after starvation dramatically increased the levels of GRK4 in both breast cancer cells and RPTC which exceeded the steady state levels. This rapid recovery of GRK4 protein do not need de novo protein synthesis because pretreatment of the cells with protein synthesis inhibitor, cycloheximide (10 μg/ml, 24 h), did not prevent this event. Expression of GRK2, another member of the GRK family, was not affected by serum starvation. Our results have shown that GRK4 is very sensitive to serum concentration in breast cancer cells as well as in RPTC. Preliminary studies suggest that rapid protein degradation rather than shutting down the protein synthesis plays a major role in this kind of GRK4 regulation. The biological significance of serum regulation of GRK4 in cancer and non-cancerous cells needs further investigation.
Biochemical Characterization of Thermophilic Dextranase from a Thermophilic Bacterium, Thermoanaerobacter pseudethanolicus (2012)

TPDex, a putative dextranase from Thermoanaerobacter pseudethanolicus, was purified as a single 70 kDa band of 7.37 U/mg. Its optimum pH was 5.2 and the enzyme was stable between pH 3.1 and 8.5 at 70 degrees C. A half-life comparison showed that TPDex was stable for 7.4 h at 70 degrees C, whereas Chaetominum dextranase (CEDex), currently used as a dextranase for sugar milling, was stable at 55 degrees C. TPDex showed broad dextranase activity regardless of dextran types, including dextran T2000, 742CB dextran, and alternan. TPDex showed the highest thermostability among the characterized dextranases, and may be a suitable enzyme for use in sugar manufacture without decreased temperature.
Intelligent Speech Web Considering User Inclination (2008)

본 논문은 음성 웹의 지능화 및 개인화를 위한 방안을 제시한다. 제안하는 시스템은 한 번의 연결에서 요청된 정보들을 하나의 트랜잭션으로 기록하고, 기록된 트랜잭션들로부터 요청된 정보들 간의 연관 규칙을 탐사하여 빈발하게 요청된 정보들의 집합을 발견하며, 시스템은 이에 기반하여 과거의 사용자들과 유사한 입력 성향을 보이는 사용자에게 발견된 빈발집합에 근거하여 관련 정보를 추천한다. 유효성 검증을 위해서 제안하는 시스템을 구축하고 실험한 결과, 제안하는 시스템이 사용자가 요청한 정보와 빈번하게 요청되었던 정보를 관련 정보로 추천함으로써 사용자의 정보 획득을 도울 수 있음을 확인하였다. In this paper, we propose a method for personalizing and intelligence of speech Web. The proposed system records information that was demanded in the past as a transaction, explores association rules from those transactions, and discovers itemsets from frequent requests. This method is to recommend relevant information, based on frequent itemsets, to users who have similar inclinations to previous users. As a result of experimenting and implementation of proposed system for verification, we confirmed that the proposed system can recommend previously frequently requested information as relevant information.
Improving molecular alignment and charge percolation in semiconducting polymer films with highly localized electronic states through tailored thermal annealing (2021)

A tailored thermal treatment on aligned P(NDI-T2) films, simultaneously inducing lamellar thickening and improved alignment, is demonstrated. Enhanced transport properties are observed and univocally addressed to the augmented orientational order.
Ferrocenyl‐Coupled N‐Heterocyclic Carbene Complexes of Gold(I): A Successful Approach to Multinuclear Anticancer Drugs (2016)

Four gold(I) carbene complexes featuring 4-ferrocenyl-substituted imidazol-2-ylidene ligands were investigated for antiproliferative and antivascular properties. They were active against a panel of seven cancer cell lines, including multidrug-resistant ones, with low micromolar or nanomolar IC<sub>50</sub> (72 h) values, according to their lipophilicity and cellular uptake. The delocalized lipophilic cationic complexes 8 and 10 acted by increasing the reactive oxygen species in two ways: through a genuine ferrocene effect and by inhibiting the thioredoxin reductase. Both complexes gave rise to a reorganization of the F-actin cytoskeleton in endothelial and melanoma cells, associated with a G1 phase cell cycle arrest and a retarded cell migration. They proved antiangiogenic in tube formation assays with endothelial cells and vascular-disruptive on real blood vessels in the chorioallantoic membrane of chicken eggs. Biscarbene complex 10 was also tolerated well by mice where it led to a volume reduction of xenograft tumors by up to 80 %.
Synthetic Antiferromagnetic Gold Nanoparticles as Bimodal Contrast Agents in MRI and CT—An Experimental In Vitro and In Vivo Study (2021)

The use of multimodal contrast agents can potentially overcome the intrinsic limitations of individual imaging methods. We have validated synthetic antiferromagnetic nanoparticles (SAF-NPs) as bimodal contrast agents for in vitro cell labeling and in vivo cell tracking using magnetic resonance imaging (MRI) and computed tomography (CT). SAF-NP-labeled cells showed high contrast in MRI phantom studies (r<sub>2</sub>* = 712 s<sup>-1</sup> mM<sup>-1</sup>), while pelleted cells showed clear contrast enhancement in CT. After intravenous SAF-NP injection, nanoparticles accumulated in the liver and spleen, as visualized in vivo by significant MRI contrast enhancement. Intravenous injection of SAF-NP-labeled cells resulted in cell accumulation in the lungs, which was clearly detectable by using CT but not by using MRI. SAF-NPs proved to be very efficient cell labeling agents for complementary MRI- and CT-based cell tracking. Bimodal monitoring of SAF-NP labeled cells is in particular of interest for applications where the applied imaging methods are not able to visualize the particles and/or cells in all organs.
Some surface characteristics and gas interactions of Apollo 14 fines and rock fragments. (1972)

Comprehensive survey of the physical surface characteristics of Apollo 14 fines, two fragments of a breccia (14321), and a crystalline rock (14310). The survey was carried out with optical and both scanning and transmission electron microscopy and by studying the adsorption of a variety of gases including nitrogen, hydrogen, and water vapor. Our objective in the optical microscope study was to relate the visible geological and petrological features to the surface properties. Electron microscopy particularly helped relate surface roughness and particle fusion to gas adsorption and pore structure. The fine sample (14163,111) had a surface area of 0.210 sq m/g and a helium density of 2.9 g/cc. Similar values have been observed with breccia fragments. Other observations include physical adsorption of molecular hydrogen at low temperatures and of water vapor at ambient temperatures. It is concluded that these particular lunar materials, while capable of adsorbing water vapor, do not retain it for any significant time at low pressures, nor, under lunar conditions, is there any indication of absorption or penetration.
Controversies surrounding percutaneous coronary intervention in the diabetic patient (2016)

Diabetic patients with coronary artery disease are common and complex, with an aggressive progression of atherosclerosis, increased rate of stent complications, and increased rates of incomplete revascularization in multivessel disease compared to non-diabetic patients. In this review, we first discuss the pathophysiologic elements of insulin resistance and presentations of coronary artery disease in diabetic patients. Next, we outline the evolution and present the data on revascularization strategies on diabetic patient outcomes. The overall conclusion of our review is that a strategy of complete and durable revascularization and guideline-directed medical therapy currently provides the best possible chance at closing the gap between outcomes in patients with and without diabetes.
Computational modeling of tactoid dynamics in chromonic liquid crystals (2017)

Motivated by recent experiments, the isotropic-nematic phase transition in chromonic liquid crystals is studied. As temperature decreases, nematic nuclei nucleate, grow, and coalesce, giving rise to tactoid microstructures in an isotropic liquid. These tactoids produce topological defects at domain junctions (disclinations in the bulk or point defects on the surface). We simulate such tactoid equilibria and their coarsening dynamics with a model using degree of order, a variable length director, and an interfacial normal as state descriptors. We adopt Ericksen's work and introduce an augmented Oseen-Frank energy, with non-convexity in both interfacial energy and the dependence of the energy on the degree of order. A gradient flow dynamics of this energy does not succeed in reproducing some simple expected feature of tactoid dynamics. Therefore, a strategy is devised based on continuum kinematics and thermodynamics to represent such features. The model is used to predict tactoid nucleation, expansion, and coalescence during the process of phase transition. We reproduce observed behaviors in experiments and perform an experimentally testable parametric study of the effect of bulk elastic and tactoid interfacial energy parameters on the interaction of interfacial and bulk fields in the tactoids.
Artificial granularity in two-dimensional arrays of nanodots fabricated by focused-electron-beam-induced deposition (2010)

We have prepared 2D arrays of nanodots embedded in an insulating matrix by means of focused-electron-beam-induced deposition using the W(CO)(6) precursor. By varying the deposition parameters, i.e. the electron beam current and energy and the raster constant, we obtain an artificial granular material with tunable electrical properties. The analysis of the temperature dependence of the conductivity and of the current-voltage characteristic suggests that the transport mechanism is governed by electron tunneling between artificial grains. In order to understand the nature of the granularity and thus the microstructural origin of the electronic transport behavior, we perform TEM and micro-Raman investigations. Independent of the deposition parameters, TEM measurements show that the dots are constituted of amorphous tungsten carbide clusters embedded in an amorphous carbonaceous matrix. Micro-Raman spectra show two peaks, around 690 and 860 cm(-1) associated with the W-C stretching modes. Higher frequency peaks give information on the composition of the matrix. In particular, we measure a peak at about 1290 cm(-1), which is associated with sp(3) carbon bonds. Furthermore we detect the so-called D and G peaks, at about 1350 and 1560 cm(-1), associated with the vibration modes of the sp(2) carbon bonds. The analysis of the position of the peaks and of their relative intensity suggests that the composition of the matrix is between nanocrystalline graphite and amorphous carbon.
Microstrutured fibers with germanium doped core components (2009)

The paper reports preparation and applicative aspects of two types of index guiding microstructured fibers (MOFs) with germanium doped cores. The first fiber type has a solid core with graded germanium profile. It shows a high photosensitivity compared to pure silica MOFs. We inscribed high-quality Bragg gratings with a reflectivity of 73% without hydrogen loading. The solid core germanium doped MOF was spliced with standard silica fiber. The minimum splice loss was about 1 dB at 1550 &#956;m wavelength. A more complex MOF type was prepared with germanium doped holey core in a silica holey cladding. The germanium doped core area includes seven holes in hexagonal arrangement with equal diameter and pitch sizes. The holey core propagates a large area annulus mode. We show the suitability of this MOF for chemical gas sensing by filling the core cavities with hydrocarbon analytes.
The Taxonomy of Spatial Typologies: A Proposal for an Analytical Language (2020)

As the phenomenon of globalization permeates across political and socio-cultural boundaries, one of its major effects is an increasingly homogenized spatial landscape. Lefebvre noted spatial production is “secreted” cultural production, so globalization is tantamount to an increasingly homogenized world culture. Designers who push back on spatial globalization eschew Modernism’s break from the past or Post-Modernism’s poly-narrative but literal u-topic emphasis, utilizing a design approach based on site-specific contextual and historic factors ranging from climate to socio-culturally produced typologies. Spatial typologies are synchronous with spatial production: their emergence, evolution and extinction exemplify a society’s material culture at specific places and times. e.g., nearly 46 centuries ago, Egyptian pyramids emerged as the salient funerary typology; thousands of years later, department stores typified the logic of capital, as the Industrial Revolution produced spaces that addressed the consumer demands of a growing bourgeoisie class. The emergence of Post-Modernism in arts and social sciences in the 1960s signaled a more liberating design approach because it embraced alternative design directions, including the reincorporation of traditional praxes of spatial production such as typologies. Rudofsky, in Architecture without Architects, reminded us that spatial production was not the exclusive domain of the formally trained designer but, more importantly, that vernacular space itself was a legible representation of a society’s values; typologies were once again important and merited documenting. Later, Pevsner noted in A History of Building Types “there is…no history of building types in existence.” Emulating the science of taxonomy, the paper proposes systematic criteria for identifying and categorizing typologies. In this approach, emphasis is placed on tracing changes in spatial morphologies over time, such that spatial relationships are revealed between earlier antecedent typologies and their contemporary iterations. This is possible because, like genetics, typologies reveal qualifying components that may illustrate iterative mutations, revealing “evolutionary drifts” or “selection processes.”
Asymmetric Rhodium-Catalysed Addition of Arylboronic Acids to Acyclic Unsaturated Esters Containing a Basic γ-Amino Group (2012)

Application of the Miyaura–Hayashi rhodium-catalysed addition of aryl boronic acids to acyclic unsaturated esters featuring basic centres to yield γ-amino butyric acids incorporating a substituted β-phenyl group is described. Unoptimised isolated yields and enantiomeric excesses vary from moderate to excellent, and the method tolerates a variety of substitution patterns and a range of functionality.
Antibiased Electrostatic RF MEMS Varactors and Tunable Filters (2010)

This paper presents a new approach for substantially enhancing the linearity and reducing the effects of bias noise for electrostatic RF microelectromechanical systems (MEMS) devices. The proposed method relies on applying bias voltages with opposite polarities to cancel the dynamic vibration of the MEMS structures. In this paper, the method has been applied to a shunt RF MEMS varactor and a MEMS tunable evanescent-mode tunable filter. In the first case, the shunt MEMS varactor is split into two identical parts that are biased with opposite voltages. This results in almost complete cancelation of the odd-order modulation components, leading to 20-28-dB linearity enhancement depending on the noise and the design. Analytical results, a computer-aided design model and measurements validate the proposed approach. In the tunable filter case, opposite bias voltages are applied on the tuners of its resonators. Simulated and measured results are also presented in this case. Measurements show a sideband reduction as high as 13 dB. In both cases, the effectiveness of the proposed method in the presence of fabrication uncertainties are also considered.
Search for new phenomena in final states with two opposite-charge, same-flavor leptons, jets, and missing transverse momentum in pp collisions at $$ \sqrt{s}=13 $$ TeV (2018)

A bstract Search results are presented for physics beyond the standard model in final states with two opposite-charge, same-flavor leptons, jets, and missing transverse momentum. The data sample corresponds to an integrated luminosity of 35.9 fb −1 of proton-proton collisions at $$ \sqrt{s}=13 $$ <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:msqrt> <mml:mi>s</mml:mi> </mml:msqrt> <mml:mo>=</mml:mo> <mml:mn>13</mml:mn> </mml:math> TeV collected with the CMS detector at the LHC in 2016. The analysis uses the invariant mass of the lepton pair, searching for a kinematic edge or a resonant-like excess compatible with the Z boson mass. The search for a kinematic edge targets production of particles sensitive to the strong force, while the resonance search targets both strongly and electroweakly produced new physics. The observed yields are consistent with the expectations from the standard model, and the results are interpreted in the context of simplified models of supersymmetry. In a gauge mediated supersymmetry breaking (GMSB) model of gluino pair production with decay chains including Z bosons, gluino masses up to 1500–1770 GeV are excluded at the 95% confidence level depending on the lightest neutralino mass. In a model of electroweak chargino-neutralino production, chargino masses as high as 610 GeV are excluded when the lightest neutralino is massless. In GMSB models of electroweak neutralino-neutralino production, neutralino masses up to 500-650 GeV are excluded depending on the decay mode assumed. Finally, in a model with bottom squark pair production and decay chains resulting in a kinematic edge in the dilepton invariant mass distribution, bottom squark masses up to 980–1200 GeV are excluded depending on the mass of the next-to-lightest neutralino.
Leptin-Induced Nuclear Translocation of STAT3 Immunoreactivity in Hypothalamic Nuclei Involved in Body Weight Regulation (2001)

Leptin is involved in the hypothalamic control of food intake and body weight. Fos immunohistochemistry has been used to functionally map leptin target neurons involved in these regulatory processes. However, only a subset of hypothalamic neurons expressing the long form of the leptin receptor (Ob-Rb) also coexpress the neuronal activation marker Fos after leptin stimulation. To functionally map all leptin target neurons, regardless of whether leptin-mediated neuronal activation or inhibition occurs, we immunohistochemically investigated the leptin-induced nuclear translocation of the signal transducer and activator of transcription molecule STAT3, which represents a crucial step in the regulation of leptin-dependent gene expression. As proven by colocalization studies with the nuclear 4',6-diamidino-2-phenylindole dilactate stain, intracerebroventricular leptin treatment, but not intracerebroventricular application of pyrogen-free saline, induced a time-dependent nuclear translocation of STAT3 immunoreactivity in hypothalamic nuclei, with strong nuclear STAT3 signals detectable in the arcuate nucleus, the lateral hypothalamus, and the ventromedial and dorsomedial hypothalamic nuclei. This leptin-induced STAT3 translocation pattern proved to be distinct from that induced by interleukin-6, another cytokine using STAT3 in its signaling pathway. Combined immunohistochemical STAT3 and Fos detection after leptin treatment revealed a higher number of STAT3-positive than Fos-positive cell nuclei in the aforementioned hypothalamic structures and showed that Fos immunoreactivity colocalized only in a subset of all leptin-responsive STAT3 nuclei. These results suggest that the detection of nuclear STAT3 immunoreactivity represents a new neuroanatomical tool to functionally map central leptin actions. They further support the importance of ventrally located caudal hypothalamic structures representing the main leptin targets involved in body weight regulation.
Multiple Color Inorganic Thin‐Film Phosphor, RE‐Doped Amorphous Gallium Oxide (RE = Rare Earth: Pr, Sm, Tb, and Dy), Deposited at Room Temperature (2018)

Multi‐color inorganic thin‐film phosphors are deposited at room temperature on glass substrates using ultra‐wide bandgap amorphous gallium oxide ( a ‐GO) as a host material. All of the a ‐GO films doped with four kinds of rare‐earth ions ( a ‐GO: RE x ) emit visible light, originating from the RE ions, by 238–257 nm ultraviolet light excitation; that is, blue &amp; red emission from RE = Pr 3+ , red from Sm 3+ , green from Tb 3+ , and blue and yellow from Dy 3+ . The a ‐GO:Pr x and a ‐GO:Tb x films emit bright light clearly visible by human eyes. Although the internal quantum efficiencies of unannealed a ‐GO:Pr 0.02 and a ‐GO:Tb 0.02 films are 0.2 and 1.8%, respectively, those are improved to 2.4 and 3.8 %, respectively, by post‐deposition thermal annealing in O 2 at 400 °C through desorption of weakly‐bonded oxygen and structural relaxation. On the other hand, the annealing temperature to improve photoluminescence is limited by crystallization (onset temperatures 400–500 °C).