gebhart/patrepeval huggingface dataset:
The Effect of Cyclooxygenase Inhibitors on the Low Filling Rate Cystometrogram in Urethane Anesthetized Rats (1985)

The effect of two widely used cyclooxygenase inhibitors (indomethacin and ketoprofen) on the low filling rate cystometrogram was investigated in urethane anesthetized rats. Both indomethacin and ketoprofen significantly increased volume threshold to elicit neurogenic bladder contractions without affecting their amplitude. Also the pressure threshold was unaffected by both drugs while the volume/pressure relationship was significantly flattened compared to controls. These findings indicate that acute suppression of arachidonic acid metabolism along the cyclooxygenase pathway increases the micturition threshold without affecting bladder muscle contractility and/or neurotransmission along the efferent pathway of the micturition reflex.
Clinical, neuroimaging, and molecular spectrum of TECPR2 ‐associated hereditary sensory and autonomic neuropathy with intellectual disability (2021)

Bi-allelic TECPR2 variants have been associated with a complex syndrome with features of both a neurodevelopmental and neurodegenerative disorder. Here, we provide a comprehensive clinical description and variant interpretation framework for this genetic locus. Through international collaboration, we identified 17 individuals from 15 families with bi-allelic TECPR2-variants. We systemically reviewed clinical and molecular data from this cohort and 11 cases previously reported. Phenotypes were standardized using Human Phenotype Ontology terms. A cross-sectional analysis revealed global developmental delay/intellectual disability, muscular hypotonia, ataxia, hyporeflexia, respiratory infections, and central/nocturnal hypopnea as core manifestations. A review of brain magnetic resonance imaging scans demonstrated a thin corpus callosum in 52%. We evaluated 17 distinct variants. Missense variants in TECPR2 are predominantly located in the N- and C-terminal regions containing β-propeller repeats. Despite constituting nearly half of disease-associated TECPR2 variants, classifying missense variants as (likely) pathogenic according to ACMG criteria remains challenging. We estimate a pathogenic variant carrier frequency of 1/1221 in the general and 1/155 in the Jewish Ashkenazi populations. Based on clinical, neuroimaging, and genetic data, we provide recommendations for variant reporting, clinical assessment, and surveillance/treatment of individuals with TECPR2-associated disorder. This sets the stage for future prospective natural history studies.
Evolution of the conductive filament with cycling in TaOx-based resistive switching devices (2020)

Physical changes occurring in TiN/TaO2.0±0.2/TiN resistive random-access memory devices after prolonged cycling have been analyzed by two scanning transmission electron microscopy modalities: high angle annular dark field and x-ray energy dispersive spectroscopy. In just formed devices, filaments had a shape of a 10 nm diameter Ta-enriched column with the O-rich gap next to electrodes, which was positively biased during electroformation. Devices that failed by stuck-in-high resistance state mode exhibited Ta depletion and oxygen interdiffusion at interfaces with both electrodes akin to effects observed in complementary switching devices. Initially narrow Ta-rich filaments broadened into ∼50 nm diameter columns showing speckled contrast due to phase separation. In devices that failed by stuck-in-low resistance state mode, we have observed a strong Ta-enriched sub-filament bridging the gap. The amount of oxygen in the TiN anode in the vicinity of the filament has not changed significantly between as-formed and failed devices, thus indicating that oxygen is not lost during switching. All devices at the end of endurance exhibited interdiffusion of O into TiN and Ti and N into TaOx.
Identification and Heterologous Expression of the Albucidin Gene Cluster from the Marine Strain Streptomyces Albus Subsp. Chlorinus NRRL B-24108 (2020)

Herbicides with new modes of action and safer toxicological and environmental profiles are needed to manage the evolution of weeds that are resistant to commercial herbicides. The unparalleled structural diversity of natural products makes these compounds a promising source for new herbicides. In 2009, a novel nucleoside phytotoxin, albucidin, with broad activity against grass and broadleaf weeds was isolated from a strain of <i>Streptomyces albus</i> subsp. <i>chlorinus</i> NRRL B-24108. Here, we report the identification and heterologous expression of the previously uncharacterized albucidin gene cluster. Through a series of gene inactivation experiments, a minimal set of albucidin biosynthetic genes was determined. Based on gene annotation and sequence homology, a model for albucidin biosynthesis was suggested. The presented results enable the construction of producer strains for a sustainable supply of albucidin for biological activity studies.
Supplementary Figure 2 from A687V EZH2 Is a Driver of Histone H3 Lysine 27 (H3K27) Hypertrimethylation (2023)

&lt;p&gt;Supplementary Figure 2: Alignment of all human SET domain methyltransferases&lt;/p&gt;
High curvature bending characterization of ultra-thin chips and chip-on-foil assemblies (2013)

Ultra-thin chips of less than 20µm become flexible, allowing integration of silicon IC technology with highly flexible electronics. This combination allows for highly intelligent products of unprecedented thinness, flexibility and cost. Examples include sensor systems integrated into food packaging or healthcare and sport monitoring tags as wearable patches or even directly in clothing textile. During use the ultra-thin chips in these products can be bent to a very high curvature, which puts a large strain on the chips. In this paper the strength of ultra-thin chips at very high curvatures is evaluated, using a modified four-point bending method. Stand-alone ultra-thin chips are evaluated which achieve a minimum bending radius below 1mm, as well as assemblies containing integrated ultra-thin chips. The effect of chip thickness, bending direction and backside finish on strength and minimum bending radius is investigated using the developed method. The difference between blank ultra-thin silicon dies and daisy chain ultra-thin chips with bond pads and bumps is highlighted. Finally the high curvature behaviour is investigated of ultra-thin chips that were integrated on low-cost polyester foil substrates using several different low-temperature integration techniques. The excellent suitability of these ultra-thin chips on low-cost foil assemblies for highly flexible large area devices is shown.
Understanding mobile money grievances from tweets (2019)

Access to financial services through a mobile phone, known as Mobile Financial Services (MFS), creates an opportunity to expand the reach of financial services to the 1.7 billion unbanked adults worldwide. Nevertheless, MFS adoption has been inconsistent, which motivates a need to identify the challenges that MFS users confront in different countries. In this work, we explore the Twitter as a potential data source to understand such challenges. More broadly, we assess whether (and how) publicly available Twitter data can augment the findings of expensive, large-scale research studies on MFS barriers. Our Qualitative Content Analysis of 9,000 mobile money grievance tweets that were extracted from 54 MFS customer care twitter feeds across six countries reveals service and access issues, incorrect transactions, and fraud as three main challenges MFS users report on Twitter. We discuss the nuances around these challenges and the substantial differences between the common issues reported in different countries. Ultimately, we conclude that Twitter data can elucidate the challenges of MFS adoption and also that it can augment the results of other types of MFS studies.
A protease protection assay for the detection of internalized alpha-synuclein pre-formed fibrils (2021)

Alpha-synuclein pre-formed fibrils (PFFs) represent a promising model system for the study of cellular processes underlying cell-to-cell transmission of alpha-synuclein proteopathic aggregates. However, the ability to differentiate the fate of internalized PFFs from those which remain in the extracellular environment remains limited due to the propensity for PFFs to adhere to the cell surface. Removal of PFFs requires repeated washing and/or specific quenching of extracellular fluorescent PFF signals. In this paper we present a new method for analyzing the fate of internalized alpha-synuclein. We inserted a tobacco etch virus (TEV) protease cleavage site between alpha-synuclein and green fluorescent protein and subjected cells to brief treatment with TEV protease after incubation with tagged PFFs. As the TEV protease is highly specific, non-toxic, and active under physiological conditions, protection from TEV cleavage can be used to distinguish internalized PFFs from those which remain attached to the cell surface. Using this experimental paradigm, downstream intracellular events can be analyzed via live or fixed cell microscopy as well as by Western blotting. We suggest that this method will be useful for understanding the fate of PFFs after endocytosis under various experimental manipulations.
Computational Quantification of Complex Fundus Phenotypes in Age-Related Macular Degeneration and Stargardt Disease (2011)

Automated quantification of fundus phenotypes was achieved, and the results show that the method can differentiate AMD from SD, two distinctly different genetically associated disorders, by quantifying the properties of the bright lesions (drusen and flecks) in their fundus images, even when the images were visually selected to be similar. Quantification of fundus phenotypes may allow recognition of new phenotypes, correlation with new genotypes and may measure disease-specific biomarkers to improve management of patients with AMD or SD.
Elastomeric and soft conducting microwires for implantable neural interfaces (2015)

Current designs for microelectrodes used for interfacing with the nervous system elicit a characteristic inflammatory response that leads to scar tissue encapsulation, electrical insulation of the electrode from the tissue and ultimately failure. Traditionally, relatively stiff materials like tungsten and silicon are employed which have mechanical properties several orders of magnitude different from neural tissue. This mechanical mismatch is thought to be a major cause of chronic inflammation and degeneration around the device. In an effort to minimize the disparity between neural interface devices and the brain, novel soft electrodes consisting of elastomers and intrinsically conducting polymers were fabricated. The physical, mechanical and electrochemical properties of these materials were extensively characterized to identify the formulations with the optimal combination of parameters including Young's modulus, elongation at break, ultimate tensile strength, conductivity, impedance and surface charge injection. Our final electrode has a Young's modulus of 974 kPa which is five orders of magnitude lower than tungsten and significantly lower than other polymer-based neural electrode materials. In vitro cell culture experiments demonstrated the favorable interaction between these soft materials and neurons, astrocytes and microglia, with higher neuronal attachment and a two-fold reduction in inflammatory microglia attachment on soft devices compared to stiff controls. Surface immobilization of neuronal adhesion proteins on these microwires further improved the cellular response. Finally, in vivo electrophysiology demonstrated the functionality of the elastomeric electrodes in recording single unit activity in the rodent visual cortex. The results presented provide initial evidence in support of the use of soft materials in neural interface applications.
The Drift Table (2005)

The Interaction Research Studio was invited to show the Drift Table in a dedicated space between the entrance hall and main galleries as part of the “A Picture of Britain” exhibition at the Tate Britain. The curator estimated that about 10,000 people interacted with the piece.
Exploring the potential of the novel imidazole‐4,5‐dicarboxyamide chemical exchange saturation transfer scaffold for pH and perfusion imaging (2022)

Here, we describe and assess the potential of 14 newly synthesized imidazole-4,5-dicarboxyamides (I45DCs) for pH and perfusion imaging. A number of these aromatic compounds possess large labile proton chemical shifts (up to 7.7 ppm from water) because of their intramolecular hydrogen bonds and a second labile proton to allow for chemical exchange saturation transfer (CEST) signal ratio-based pH measurements. We have found that the contrast produced is strong for a wide range of substitutions and that the inflection points in the CEST signal ratio versus pH plots used to generate concentration-independent pH maps can be adjusted based on these subsitutions to tune the pH range that can be measured. These I45DC CEST agents have advantages over the triiodobenzenes currently employed for tumor and kidney pH mapping, both preclinically and in initial human studies. Finally, as CEST MRI combined with exogenous contrast has the potential to detect functional changes in the kidneys, we evaluated our highest performing anionic compound (I45DC-diGlu) on a unilateral urinary obstruction mouse model and observed lower contrast uptake in the obstructed kidney compared with the unobstructed kidney and that the unobstructed kidney displayed a pH of ~ 6.5 while the obstructed kidney had elevated pH and an increased range in pH values. Based on this, we conclude that the I45DCs have excellent imaging properties and hold promise for a variety of medical imaging applications, particularly renal imaging.
Surface affinity role in graphoepitaxy of lamellar block copolymers (2016)

Overcoming the optical limitations of 193-nm immersion lithography can be achieved using directed self-assembly (DSA) of block-copolymers (BCPs) as a low-cost and versatile complementary technique. The goal of this paper is to investigate the potential of DSA to address line and space (L/S) high-resolution patterning by performing the density multiplication of lines with the graphoepitaxy approach. As surface affinity is a key parameter in self-assembly, three variations, or “flavors,” of DSA template affinity are investigated regarding several success criteria such as morphology control or defectivity. More precisely, both the methodology to register DSA defects and the impact of process parameters on defectivity are detailed. Using the 300-mm pilot line available in LETI and Arkema’s advanced materials, we investigate process optimization of DSA line/space patterning of a 38-nm period lamellar PS-b-PMMA BCP (L38). Our integration scheme is based on BCP self-assembly inside organic hard mask guiding patterns obtained using 193i-nm lithography. Defect analysis coupled with the fine tuning of process parameters (annealing, brush material) provided the optimum conditions for the L38 self-assembly. Using such conditions, DSA using the three affinity flavors is investigated by means of SEM top-view and cross-section review. Lithographic performances of one selected flavor are then evaluated with the comparison of process windows function of either commensurability, morphology, or roughness. This work is meant as a guideline for the graphoepitaxy optimization of materials and process parameters on a 300-mm platform.
CLEF 2011 (2012)

This paper describes the activities and the outcomes of the conference and evaluation labs of CLEF 2011.
Comparison of Linear and Nonlinear Approaches on Single Trial ERP Detection in Rapid Serial Visual Presentation Tasks (2006)

In this paper, we describe a system for detecting encephalography (EEG) signatures of visual recognition events evoked in a single trial during rapid serial visual presentation (RSVP). In order to investigate the viability of nonlinear approaches in EEG detection and assess the performance comparison, we applied three classifiers (linear logistic regression model, Laplacian classifier, and spectral maximum mutual information projection) in the detection tasks. The EEG was recorded using 32 electrodes during the rapid image presentation (50ms/100ms per image). Subjects were instructed to push a button when they recognize a target image. The results suggest that while the detection of single trial EEG-based recognition is possible, taking advantage of the nonlinear techniques requires data representation that would overcome the non-stationarity of the EEG signals.
Polymerisation mit Metallocenen in Wasser: Ein Vorurteil ist widerlegt (2001)

Normalerweise unter strengem Luft- und Feuchtigkeitsausschluss zu handhabende Metallocenkatalysatoren wie [TiCp*(OMe)3] (1, Cp*=Pentamethylcyclopentadienyl) wurden erfolgreich für die wässrige Emulsionspolymerisation von syndiotaktischem Polystyrol eingesetzt. Dabei wird der Katalysator im Inneren eines Monomertröpfchens verkapselt und so vor dem Angriff durch Wasser geschützt.
Thermal Characterisation of Hybrid, Flip-Chip InP-Si DFB Lasers (2023)

WA detailed thermal analysis of a hybrid, flip-chip InP-Si DFB laser is presented in this work. The lasers were experimentally tested at different operating temperatures, which allowed for deriving their thermal performance characteristics: the temperature dependence of threshold current, lasing slope, and output spectrum. Using these data, the laser thermal resistance was calculated (<i>R</i><sub>th</sub> = 75.9 K/W), which allows for predicting the laser temperature during operation. This metric is also used to validate the thermal finite element models of the laser. A sensitivity study of the laser temperature was performed using these models, and multiple routes for minimising both the laser thermal resistance and thermal coupling to the carrier die are presented. The most effective way of decreasing the laser temperature is the direct attachment of a heat sink on the laser top surface.
Acute Exertional Compartment Syndrome of the Forearm (2006)

Dhawan, Vikas MD, PhD; Borschel, Gregory H. MD; Brown, David L. MD, FACS Author Information
Heterogeneous architecture models for interconnect-motivated system design (2000)

On-chip interconnect demand is becoming the dominant factor in modern processor performance and must be estimated early in the design process. This paper presents a set of heterogeneous architectural models that combines architecture description and Rent's rule-based wiring models. These architecture models allow flexible heterogeneous system specifications, enabling investigations of prospective designs in different technology scenarios. Comparisons against actual data demonstrate the models' effectiveness for architecture explorations with highly accurate estimations of local and global wiring demand, as well as chip area and cycle time. Simulation of two candidate system designs reveal trends in interconnect delay with increasing architectural complexity, and confirm the need for high computational locality and short global wires for future architectures.
SAR Study of a Subtype Selective Allosteric Potentiator of Metabotropic Glutamate 2 Receptor, N‐(4‐Phenoxyphenyl)‐N‐(3‐pyridinylmethyl)ethanesulfonamide. (2004)

Abstract For Abstract see ChemInform Abstract in Full Text.
Expression of Prostasin and Protease Nexin-1 in Rhesus Monkey (Macaca mulatta) Endometrium and Placenta During Early Pregnancy (2006)

Serine proteases have been documented to play key roles in uterine matrix turnover and trophoblastic invasion during implantation. Roles of prostasin serine protease in these processes, however, are currently unclear. The present study was first conducted to investigate the colocalization of prostasin and its cognate serpin, protease nexin-1 (PN-1), in rhesus monkey endometrium and placenta on days 12, 18, and 26 of pregnancy by using in situ hybridization (ISH) and immunohistochemistry. With ISH, expression of prostasin mRNA was intensely localized in the glandular epithelium on days 12 and 18 and in the placental villi, trophoblastic column, trophoblastic shell, and fetal-maternal border on days 18 and 26. With the progress of pregnancy, expression level in the glandular epithelium was significantly decreased, and the accumulation in the placental compartments was further increased. In addition, the stroma and arterioles exhibited modest levels of prostasin signals. However, expression level of PN-1 in these compartments on adjacent sections in the three stages of early pregnancy was weak or below the level of detection. Prostasin protein expression in the endometrium was found to be consistent with the distribution patterns revealed in the ISH experiments. It may be suggested from these results that prostasin is involved in endometrial epithelial morphology establishment, tissue remodeling, and trophoblastic invasion during early pregnancy. The cognate serpin PN-1 was not coordinately expressed along with prostasin, creating a tissue environment favorable for proteolytic activities of prostasin during early pregnancy events.
High-Throughput Screening for Small-Molecule Inhibitors of Plasmodium falciparum Glucose-6-Phosphate Dehydrogenase 6-Phosphogluconolactonase (2012)

Plasmodium falciparum causes severe malaria infections in millions of people every year. The parasite is developing resistance to the most common antimalarial drugs, which creates an urgent need for new therapeutics. A promising and attractive target for antimalarial drug design is the bifunctional enzyme glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase (PfGluPho) of P. falciparum, which catalyzes the key step in the parasites' pentose phosphate pathway. In this study, we describe the development of a high-throughput screening assay to identify small-molecule inhibitors of recombinant PfGluPho. The optimized assay was used to screen three small-molecule compound libraries-namely, LOPAC (Sigma-Aldrich, 1280 compounds), Spectrum (MicroSource Discovery Systems, 1969 compounds), and DIVERSet (ChemBridge, 49 971 compounds). These pilot screens identified 899 compounds that inhibited PfGluPho activity by at least 50%. Selected compounds were further studied to determine IC(50) values in an orthogonal assay, the type of inhibition and reversibility, and effects on P. falciparum growth. Screening results and follow-up studies for selected PfGluPho inhibitors are presented. Our high-throughput screening assay may provide the basis to identify novel and urgently needed antimalarial drugs.
Implantable Cardioverter Defibrillators in Patients with Valvular Cardiomyopathy (2012)

Patients with VCM who undergo ICD implantation for SCD prevention have similar appropriate ICD discharge rates and mortality as do those with ICM and DCM. These data are hypothesis generating and deserve confirmation with large-scale prospective studies.