gebhart/patrepeval huggingface dataset:
Enzymatic transformation products of phloretin as potent antiadipogenic compounds (2021)

Enzymatic structure modification of the representative chalcone phloretin (1) with polyphenol oxidase from Agaricus bisporus origin produced 2 new biphenyl-type phloreoxin (2) and phloreoxinone (3), and a previously undescribed (2R)-5,7,3',5'-tetrahydroxyflavanone (4). The structure of these new oxidized products 2-4 elucidated by interpreting the spectroscopic data (NMR and FABMS) containing the absolute stereochemistry is established by the analysis of the circular dichroism spectrum. Compared to the original phloretin, the new products (2) and (3) showed highly improved antiadipogenic potencies both toward pancreatic lipase and accumulation of 3T3-L1 cells. Also, phloreoxin (2) effectively inhibited the expression of C/EBPβ, PPARγ, and aP2 at the mRNA level in the 3T3 adipocytes. Thus, phloreoxin (2), containing a biphenyl moiety catalyzed by A. bisporus polyphenol oxidase, have the potential to influence the antiadipogenic capacity.
Genome Editing Via NHEJ-Mediated Targeted Integration Retains Engraftment Potential of Edited Hematopoietic Stem Cells (2018)

Abstract The ability to edit the genome of long-term-engrafting hematopoietic stem cells (LT-HSCs) would provide a curative therapy for a wide range of hematological disorders. While mixed LT/ST-HSC (ST, short-term) pools edited via non-homologous end joining (NHEJ) yield engraftable transgenic cells, pools made transgenic via homology-dependent repair (HDR) do not. This observation is likely explained by the absence of DNA replication required for HDR in LT-HSCs. Quiescence renders LT-HSCs refractory to transgene insertion via HDR, with the transgene-positive population consisting overwhelmingly of actively dividing, short-term-engrafting HSCs. In contrast to HDR, NHEJ is a DNA repair mechanism active in non-dividing cells. We hypothesized that CRISPR/Cas9-mediated transgenesis of HSC pools via NHEJ-mediated targeted integration (NHEJ-TI) would allow modification of LT-HSCs and produce edited, long-term engrafting progeny. First, we investigated whether NHEJ-TI is a feasible method of genome editing in HSCs. Utilizing CRISPR/Cas9 to induce a DSB in the AAVS1 (PPP1R12C) locus and a self-complementary rAAV6 (scAAV6) to deliver a GFP expression cassette dependent on the endogenous promoter, we optimized RNP nucleofection, scAAV6 infection, and HSC culture conditions for efficient NHEJ-TI in HSCs. Flow cytometry for eGFP and in-out PCR was used to confirm transgene integration at frequencies ranging from 4-6%. Next, we investigated whether the NHEJ-TI edited pool of mixed LT/ST-HSCs contained engraftable, edited cells by injection into irradiated NOD/SCID/IL2Rg-/- (NSG) mice. We compared the engraftment potential of transgenic HSCs cultured for either 2 days (2D) or 2 hours (2H) prior to editing. While the overall editing efficiency was ~20% higher in the 2D condition, the near absence of cell prestimulation in the 2H condition resulted in increased levels of overall engraftment, doubling the total engraftment level of edited hematopoietic cells at 10 weeks post-injection. Importantly, the percentage of edited cells among the engrafted human hematopoietic cell population remained essentially unchanged relative to the starting efficiency (2D: 4.0%, 2H: 3.6%; 10 weeks post-injection). Analysis of bone marrow-resident CD34-positive cells at 16 weeks post-engraftment will reveal the fraction of edited LT-HSCs. Our results show that NHEJ-TI is a feasible method of inserting transgenes into HSCs whilst retaining their engraftment potential. To our knowledge, this is the first study to achieve successful NHEJ-mediated targeted integration in HSCs while retaining engraftment potential and (perhaps) transgenesis as well. Disclosures Uenishi: Casebia Therapeutics: Employment. Abe:Casebia Therapeutics: Employment. Mallari:Casebia Therapeutics: Employment. Scharenberg:Generation Bio: Equity Ownership; Casebia Therapeutics: Employment; Alpine Immune Sciences: Equity Ownership. Cost:Casebia Therapeutics: Employment.
Nanoparticle-Associated Surfactant Micellar Fluids: An Alternative to Crosslinked Polymer Systems (2012)

Abstract Hydraulic fracturing has become an increasingly important completion method to allow reservoirs to become economically producible and to improve the rate of hydrocarbon production. Crosslinked polymer fluids have been the most commonly used fracturing fluid. These fluids exhibit exceptional performance to initiate and propagate a fracture and carry proppant into the reservoir during a treatment. However, crosslinked polymer fluids can leave a significant amount of polymer in the fracture once a treatment is completed. Decades of improving oxidative, enzyme and other breakers for the polymer fluids has only marginally improved the amount of polymer residue left within the fracture. The result has been wells with subpar hydrocarbon recovery rates and in many geographic areas the return of large volume waterfracs. Over the past two decades surfactant-based fluids have been developed as a low-damage alternative. However, surfactant-based systems have had major limitations for hydraulic fracturing. This paper will introduce improved fluid technology that uses nanoparticles, internal breakers and low molecular weight surfactants to achieve the performance of crosslinked polymer fluids but leaves little to no gel residue. Nanoparticles have been found that uniquely associate thread-like micelles into 3-deminsional network that imparts wall-building leak-off control and fluid efficiency. The system also utilizes internal breakers that reside within the micelles and go wherever the fluid goes to insure complete viscosity reduction. The nanoparticle enhanced surfactant-based fluid technology may allow a wide range of hydraulically fractured reservoirs to produce at higher sustained rates than presently achievable, particularly for wells highly sensitive to fracture conductivity damage. This paper will present laboratory data that shows how uniquely charged nanoparticles improve surfactant-based fluid rheology and leak-off control properties. The mechanisms for the observed performance enhancements also will be discussed.
Pulsewidth dependence of laser damage in fused silica (2003)

Summary form only given. The availability of ultrafast sub-50 fs lasers and the technology of chirped-pulse amplification have extended the study of the pulsewidth dependence of damage to ultrashort time scales. Various groups have reported two remarkable features of ultrashort pulse damage. First, short pulse damage exhibits a deterministic nature as opposed to the statistical behavior for long-pulse damage. Second, damage threshold fluence is higher than the prediction from the /spl radic//spl tau/ scaling rule for pulsewidth /spl tau/<10 ps. However, these measurements display different pulsewidth dependence of damage threshold in the subpicosecond regime. None of the theoretical models proposed before can explain this dissimilarity. The discrepancy among observations was thought to arise from different experimental conditions for instance, the number of laser shots. The sample may suffer a cumulative change in material properties as it is subjected to a series of subthreshold pulses. In order to study intrinsic laser damage, and to exclude the complexity of cumulative effects, our recent measurement was done under single-shot conditions.
Constrictive Pericarditis in the Modern Era (1999)

The evolving profile of CP, with increasingly older patients and those with radiation-induced disease in the past decade, significantly affects postoperative prognosis. Long-term results of pericardiectomy are disappointing for some patient groups, especially those with radiation-induced CP. By contrast, surgery alleviates or improves symptoms in the majority of late survivors.
The calmodulin‐dependent glycogen synthase kinase from rabbit skeletal muscle (1983)

A calmodulin-dependent glycogen synthase kinase distinct from phosphorylase kinase has been purified approximately equal to 5000-fold from rabbit skeletal muscle by a procedure involving fractionation with ammonium sulphate (0-33%), and chromatographies on phosphocellulose, calmodulin-Sepharose and DEAE-Sepharose. 0.75 mg of protein was obtained from 5000 g of muscle within 4 days, corresponding to a yield of approximately equal to 3%. The Km for glycogen synthase was 3.0 microM and the V 1.6-2.0 mumol min-1 mg-1. The purified enzyme showed a major protein staining band (Mr 58 000) and a minor component (Mr 54 000) when examined by dodecyl sulphate polyacrylamide gel electrophoresis. The molecular weight of the native enzyme was determined to be 696 000 by sedimentation equilibrium centrifugation, indicating a dodecameric structure. Electron microscopy suggested that the 12 subunits were arranged as two hexameric rings stacked one upon the other. Following incubation with Mg-ATP and Ca2+-calmodulin, the purified protein kinase underwent an 'autophosphorylation reaction'. The reaction reached a plateau when approximately equal to 5 mol of phosphate had been incorporated per 58 000-Mr subunit. Both the 58 000-Mr and 54 000-Mr species were phosphorylated to a similar extent. Autophosphorylation did not affect the catalytic activity. The calmodulin-dependent protein kinase initially phosphorylated glycogen synthase at site-2, followed by a slower phosphorylation of site-1 b. The protein kinase also phosphorylated smooth muscle myosin light chains, histone H1, acetyl-CoA carboxylase and ATP-citrate lyase. These findings suggest that the calmodulin-dependent glycogen synthase kinase may be a enzyme of broad specificity in vivo. Glycogen synthase kinase-4 is an enzyme that resembles the calmodulin-dependent glycogen synthase kinase in phosphorylating glycogen synthase (at site-2), but not glycogen phosphorylase. Glycogen synthase kinase-4 was unable to phosphorylate any of the other proteins phosphorylated by the calmodulin-dependent glycogen synthase kinase, nor could it phosphorylate site 1 b of glycogen synthase. The results demonstrate that glycogen synthase kinase-4 is not a proteolytic fragment of the calmodulin-dependent glycogen synthase kinase, that has lost its ability to be regulated by Ca2+-calmodulin.
Activity, disulphate mapping and structural modelling of the fifth domain of human β2‐glycoprotein I (1992)

Complexes formed by the interaction of negatively charged phospholipids and beta 2-glycoprotein I (beta 2-I) are the target of autoantibodies in systemic lupus erythematosus. The highly positively charged fifth (C-terminal) domain of human beta 2-I was produced as a fusion protein in an Escherichia coli expression system and was shown to bind to the negatively charged phospholipid, cardiolipin, almost as well as the intact protein. In an attempt to define the 3D structure of this domain, the disulphide linkage pattern was determined and shown to be Cys 1-4, Cys 2-5 and Cys 3-6 in contradiction to an earlier report. In the light of this information, the sequence of the fifth domain of beta 2 I (beta 2-I-5) is readily aligned with that of the 16th repeat of factor H, of which the 3D structure is known, and a model of beta 2I-5 has been built by homology. On the basis of the model we suggest residues that might be the target of profitable site-directed mutagenesis in structure-function studies.
Preclinical Assessment ofFHITGene Replacement Therapy in Human Leukemia Using a Chimeric Adenovirus, Ad5/F35 (2006)

FHIT restoration in some FHIT-deficient leukemia cells induces both antiproliferative and proapoptotic effects involving the intrinsic caspase apoptotic pathway.
Electron Transport through Thin Organic Films in Metal−Insulator−Metal Junctions Based on Self-Assembled Monolayers  [J. Am. Chem. Soc. 2001, 123, 5075−5085]. (2002)

ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionORIGINAL ARTICLEThis notice is a correctionElectron Transport through Thin Organic Films in Metal−Insulator−Metal Junctions Based on Self-Assembled Monolayers [J. Am. Chem. Soc.2001, 123, 5075−5085].R. Erik Holmlin, Rainer Haag, Michael L. Chabinyc, Rustem F. Ismagilov, Adam E. Cohen, Andreas Terfort, Maria Anita Rampi, and George M. WhitesidesCite this: J. Am. Chem. Soc. 2002, 124, 29, 8762Publication Date (Web):June 21, 2002Publication History Published online21 June 2002Published inissue 1 July 2002https://pubs.acs.org/doi/10.1021/ja025104jhttps://doi.org/10.1021/ja025104jcorrectionACS PublicationsCopyright © 2002 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views742Altmetric-Citations5LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (8 KB) Get e-Alertsclose Get e-Alerts
INTERACTION OF ANTI-HLA ANTIBODIES WITH PIG XENOANTIGENS 1 (2000)

Anti-HLA class I Ab presumably cross-react with pig class I homologues. However, some plasma samples did not cross-react, suggesting that negatively cross-matched pig kidneys could be identified in the pig population for xenotransplantation in these patients. Further studies are required to precisely describe these cross-reactivities and to understand their functional significance in xenotransplantation.
Cryptococcus neoformans releases proteins during intracellular residence that affect the outcome of the fungal–macrophage interaction (2022)

<i>Cryptococcus neoformans</i> is a facultative intracellular pathogen that can replicate and disseminate in mammalian macrophages. In this study, we analyzed fungal proteins identified in murine macrophage-like cells after infection with <i>C. neoformans</i>. To accomplish this, we developed a protocol to identify proteins released from cryptococcal cells inside macrophage-like cells; we identified 127 proteins of fungal origin in infected macrophage-like cells. Among the proteins identified was urease, a known virulence factor, and others such as transaldolase and phospholipase D, which have catalytic activities that could contribute to virulence. This method provides a straightforward methodology to study host-pathogen interactions. We chose to study further Yeast Oligomycin Resistance (Yor1), a relatively uncharacterized protein belonging to the large family of ATP binding cassette transporter (ABC transporters). These transporters belong to a large and ancient protein family found in all extant phyla. While ABC transporters have an enormous diversity of functions across varied species, in pathogenic fungi they are better studied as drug efflux pumps. Analysis of <i>C. neoformans yor1Δ</i> strains revealed defects in nonlytic exocytosis, capsule size, and dimensions of extracellular vesicles, when compared to wild-type strains. We detected no difference in growth rates and cell body size. Our results indicate that <i>C. neoformans</i> releases a large suite of proteins during macrophage infection, some of which can modulate fungal virulence and are likely to affect the fungal-macrophage interaction.
ChemInform Abstract: Exploration for Large‐Scale Stereoselective Synthesis of Unusual Amino Acids by Using 4‐Phenyloxazolidin‐2‐one as a New Chiral Resolution Reagent. (1995)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Ethical and Practical Guidelines for Reporting Genetic Research Results to Study Participants (2010)

In January 2009, the National Heart, Lung, and Blood Institute convened a 28-member multidisciplinary Working Group to update the recommendations of a 2004 National Heart, Lung, and Blood Institute Working Group focused on Guidelines to the Return of Genetic Research Results. Changes in the genetic and societal landscape over the intervening 5 years raise multiple questions and challenges. The group noted the complex issues arising from the fact that technological and bioinformatic progress has made it possible to obtain considerable information on individuals that would not have been possible a decade ago. Although unable to reach consensus on a number of issues, the working group produced 5 recommendations. The working group offers 2 recommendations addressing the criteria necessary to determine when genetic results should and may be returned to study participants, respectively. In addition, it suggests that a time limit be established to limit the duration of obligation of investigators to return genetic research results. The group recommends the creation of a central body, or bodies, to provide guidance on when genetic research results are associated with sufficient risk and have established clinical utility to justify their return to study participants. The final recommendation urges investigators to engage the broader community when dealing with identifiable communities to advise them on the return of aggregate and individual research results. Creation of an entity charged to provide guidance to institutional review boards, investigators, research institutions, and research sponsors would provide rigorous review of available data, promote standardization of study policies regarding return of genetic research results, and enable investigators and study participants to clarify and share expectations for the handling of this increasingly valuable information with appropriate respect for the rights and needs of participants.
The use of ELISAs for monitoring exposure of pig herds to Brachyspira hyodysenteriae (2012)

IgG ELISAs using either WC or Bhlp29.7 as plate-coating antigens were shown to be useful for monitoring the dynamics of B. hyodysenteriae infection in grower pigs. Of the two antigens, the WC preparation tended to give better discrimination between pigs from infected and non-infected farms. Testing of meat juice was shown to have potential for identifying infected herds.
Not as simple as ABC: Tools to trigger massive transfusion in pediatric trauma (2021)

Diagnostic Tests or Criteria, Level IV.
Salivary Proteins of Russian Wheat Aphid (Hemiptera: Aphididae) (2010)

Salivary secretions play critical roles in aphid-host plant interactions and are responsible for damage associated with aphid feeding. The objectives of this study were to evaluate aspects of salivation and the salivary constituents of Diuraphis noxia (Hemiptera: Aphididae). Salivary proteins were isolated and compared from three aphid probed diets: pure water, 15% sucrose, or amino acids (100 mM serine, 100 mM methionine, 100 mM aspartic acid, and 15% sucrose). After 6 h, more aphids settled on sucrose diet compared with other diets, but there were no significant differences in the number of stylet sheaths produced per aphid after 24 h. There were differences in the amount of soluble salivary protein (watery saliva), with the greatest amount secreted in sucrose diet, followed by amino acid diet and pure water, respectively. Protein constituents secreted into sucrose and amino acid diets were compared using gel electrophoresis using standardized amounts of protein. More protein bands and bands of greater intensity were visualized from probed sucrose diet compared with probed amino acid diet, indicating qualitative differences. Phosphatase was putatively identified from D. noxia saliva from a major protein band using gel electrophoresis and mass spectrophotometry. Alkaline phosphatase activity was confirmed in sucrose diet using enzymatic assays but was not detected in aphid probed water or amino acid diets. Other peptides in sucrose diet weakly but significantly showed similarities to putative dehydrogenase and RNA helicase expressed sequence tags identified from other aphids. The implications of these findings in aphid salivation and plant-insect interactions are discussed.
Test setup for anechoic room based MIMO OTA testing of LTE terminals (2013)

Over-The-Air (OTA) performance testing is becoming a key method for Multiple-Input-Multiple-Output (MIMO) enabled devices as it allows evaluation of most aspects of the radio communication performance. This topic has become a very<br/>important research topic especially because such technology is now introduced into, for example, LTE andWiMAX systems. The main purpose of this testing is to validate that the user equipment will have a good performance in real use. CTIA, 3GPP and COST are spending a big effort in standardizing the OTA testing procedure which is much more complex than similar SISO OTA testing. The CTIA MIMO OTA SubGorup (MOSG) is comparing each proposed MIMO OTA technique to establish, first, an exact methodology for each technique and second, to ensure each of them offers equivalent results. This contribution focuses on the multi-probe anechoic chamber solution and summarizes the activities related to the MIMO OTA lab being built at Aalborg University (AAU), Denmark and the testing being performed.
Intraoperative MR-Guided Instillation of Phosphorum-32 for Cystic Craniopharyngiomas: Case Report (2003)

Intraoperative magnetic resonance imaging has been applied to a number of neurosurgical disease processes since the late 1990's. The ability to visualize the operative site in near-real time has added a significant degree of safety to the treatment of lesions such as a cystic craniopharyngioma which can be located in regions of the brain where an untoward consequence can result in significant neurological morbidity. Previous surgical techniques, although often successful, did not allow the neurosurgeon to directly visualize whether the goals of surgery had been met or whether there was an inadvertent complication associated with the surgical approach until after the event had occurred. The safe and accurate instillation of radioactive phosphorus into this cystic tumor resulted in clinical improvement and the maintenance of normal pituitary function for this patient. The extreme accuracy and safety of this surgical technique is demonstrated by the imaging examples provided.
Progress Towards Full Stabilization of an Injection Locked 10GHz Chip-Scale Mode-Locked Laser on InP (2018)

Repetition rate stabilization of an injection locked chip-scale mode-locked laser and progress towards carrier envelope offset frequency stabilization using chalcogenide waveguides for supercontinuum generation and a bulk PPLN for second harmonic generation are presented.
Chemokines and chemokine receptors required for optimal responses to anticancer chemotherapy (2014)

Depending on tumor type, stage and immunological contexture, the inhibition of chemokines or their receptors may yield positive or deleterious effects on disease progression. We have recently demonstrated in several murine models of anthracycline-based chemotherapy that the inhibition of chemokine (C-C motif) ligand 2 (CCL2) or chemokine (C-C motif) receptor 2 (CCR2) may impair the elicitation of anticancer immune responses that contribute to therapeutic success.