gebhart/patrepeval huggingface dataset:
A Method to Improve Cellular Content for Corporal Tissue Engineering (2008)

We have previously shown that penile corporal structures engineered using autologous cells are able to achieve erection, penetration, and ejaculation. However, fully functional corpora could not be engineered because of the limited cellular content present within the corporal tissue construct. In this study, we investigated whether a dynamic seeding approach would improve cellularity within the corporal tissue construct and thereby restore normal erectile function. Corporal cells were either statically or dynamically seeded on acellular corporal tissue matrices and maintained in a bioreactor system. After 48 h, the cell-matrix complexes were implanted subcutaneously in athymic mice and analyzed for cell attachment, survival, and distribution using histological and molecular techniques. Native tissues and matrices without cells served as controls. The seeded cells attached and proliferated within the sinusoidal walls of the matrices. After completing the seeding, the DNA and cellular content of the dynamically seeded matrices reached 71% of normal corpora, whereas the statically seeded matrices reached 39% of normal corpora. These findings were confirmed histologically, biochemically, and using scanning electron microscopy. This study demonstrates that dynamic cell attachment, using a bioreactor system, leads to the formation of morphologically and biochemically improved corporal tissue, which may be useful for penile reconstruction.
Elasticity and Viscosity of DNA Liquid Crystals (2020)

Concentrated solutions of blunt-ended DNA oligomer duplexes self-assemble in living polymers and order into lyotropic nematic liquid crystal phase. Using the optical torque provided by three distinct illumination geometries, we induce independent splay, twist, and bend deformations of the DNA nematic and measure the corresponding elastic coefficients <i>K</i><sub>1</sub>, <i>K</i><sub>2</sub>, and <i>K</i><sub>3</sub>, and viscosities η<sub>splay</sub>, η<sub>twist</sub>, and η<sub>bend</sub>. We find the viscoelasticity of the system to be remarkably soft, as the viscoelastic coefficients are smaller than in other lyotropic liquid crystals. We find <i>K</i><sub>1</sub> > <i>K</i><sub>3</sub> > <i>K</i><sub>2</sub>, in agreement with the elasticity of the nematic phase of flexible polymers, and η<sub>bend</sub> > η<sub>splay</sub> > η<sub>twist</sub> a behavior that is nonconventional in the context of chromonic, polymeric, and thermotropic liquid crystals, indicating a possible role of the weakness and reversibility of the DNA aggregates.
On the Antigenic Determinants of the Lipopolysaccharides of Vibrio cholerae O:1, Serotypes Ogawa and Inaba (1998)

Monoclonal, murine IgG1s S-20-4, A-20-6, and IgA 2D6, directed against Vibrio cholerae O:1 Ogawa-lipopolysaccharide exhibited the same fine specificities and similar affinities for the synthetic methyl alpha-glycosides of the (oligo)saccharide fragments mimicking the Ogawa O-polysaccharide (O-PS). They did not react with the corresponding synthetic fragments of Inaba O-PS. IgG1s S-20-4 and A-20-6 have absolute affinity constants for synthetic Ogawa mono- to hexasaccharides of from approximately 10(5) to approximately 10(6) M-1. For IgG1s S-20-4, A-20-6, and IgA 2D6, the nonreducing terminal residue of Ogawa O-PS is the dominant determinant, accounting for approximately 90% of the maximal binding energy shown by these antibodies. Binding studies of derivatives of the Ogawa monosaccharide and IgGs S-20-4 and A-20-6 revealed that the C-2 O-methyl group fits into a somewhat flexible antibody cavity and that hydrogen bonds involving the oxygen and, respectively, the OH at the 2- and 3-position of the sugar moiety as well as the 2'-position in the amide side chain are required. Monoclonal IgA ZAC-3 and IgG3 I-24-2 are specific for V. cholerae O:1 serotypes Ogawa/Inaba-LPS.1 The former did not show binding with members of either series of the synthetic ligands related to the O-antigens of the Ogawa or Inaba serotypes, in agreement with its reported specificity for the lipid/core region (1). Inhibition studies revealed that the binding of purified IgG3 I-24-2 to Ogawa-LPS might be mediated by a region in the junction of the OPS to the lipid-core region of the LPS. cDNA cloning and analysis of the anti-Ogawa antibodies S-20-4, A-20-6, and 2D6 revealed a very high degree of homology among the heavy chains. Among the light chains, no such homology between S-20-4 and A-20-6 on the one hand, and 2D6 on the other hand, exists. For the anti-Inaba/Ogawa antibodies I-24-2 and ZAC-3, their heavy chains are completely different, with some homology among the light chains.
ChemInform Abstract: Heteroatomic Influences on the π‐Facial Selectivity of Diels‐Alder Cycloadditions to Dispiro(4.0.4.4)tetradeca‐11,13‐dienes. (1995)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Loss of Sagittal Plane Correction After Removal of Spinal Implants (2000)

Spinal implant removal after long posterior fusion in adults may lead to spinal collapse and further surgery. Removal of instrumentation should be avoided or should involve partial removal of the prominent implant.
RseB Binding to the Periplasmic Domain of RseA Modulates the RseA:ςE Interaction in the Cytoplasm and the Availability of ςE·RNA Polymerase (2000)

The Escherichia coli sigmaE regulon has evolved to sense the presence of misfolded proteins in the bacterial envelope. Expression of periplasmic chaperones and folding catalysts is under the control of sigmaE RNA polymerase. The N-terminal domain of RseA sequesters sigmaE in the cytoplasmic membrane, preventing its association with core RNA polymerase. The C-terminal domain of RseA interacts with RseB, a periplasmic protein. The relative concentration of sigmaE:RseA:RseB is 2:5:1 and this ratio remains unaltered upon heat shock induction of the sigmaE regulon. Purification from crude cellular extracts yields cytoplasmic, soluble sigmaE RNA polymerase as well as membrane sequestered sigmaE.RseA and sigmaE.RseA.RseB. RseB binding to the C-terminal domain of RseA increases the affinity of RseA for sigmaE by 2- to 3-fold (Kd 50-100 nM). RseB binds also to the misfolded aggregates of MalE31, a variant of maltose binding protein that forms inclusion bodies in the periplasm. We discuss a model whereby the RseB-RiseA interaction represents a measure for misfolded polypeptides in the bacterial envelope, modulating the assembly of sigmaE RNA polymerase and the cellular heat shock response.
Preliminary Investigation Into the Effects of Cross-Flow on Low Reynolds Number Transition (2011)

The present work presents a preliminary investigation into the e ects of crossow on transition at low Reynolds numbers, an area which has essentially remained unexplored. The ow around an in nite SD7003 wing at an angle of attack of 4 is considered at a chord Reynolds numbers of 60,000, and for sweep angles ranging between 0 and 60 . A separation bubble is present on the upper surface where the ow transitions to turbulence, and both Tollmien-Schlichting (TS) waves and crossow instabilities are observed. The level of coupling between crossow and streamwise boundary layer velocity components is evaluated by projecting them along two-dimensional equivalent directions. It is established that the crossow cannot be decoupled from the streamwise evolution for sweep angles between about 10 and 40 due to strong non-linear interactions that take place after the laminar boundary layer separates. Hence, in separation-induced transition at low Reynolds numbers, it is not possible to treat streamwise and crossow instabilities independently for wings at intermediate sweep angles, and predicting the mixed transition cannot be reduced to treating the disturbances of each component separately. Furthermore, the type of transition (TS dominated, crossow dominated, or mixed) is a priori unknown, as soon as the ow is slightly misaligned with the wing’s chord|an important presumption for the study of unsteady ows encountered in MAVs and animal locomotion.
Association of CSF Biomarkers With Hippocampal-Dependent Memory in Preclinical Alzheimer Disease (2021)

Tests designed to tax hippocampal function are sensitive to subtle individual differences in memory among CU and correlate with early AD-associated biomarker changes in CSF. These tests may offer utility for identifying CU with preclinical AD pathology.
Measurement of CP violation and constraints on the CKM angle γ in B±→DK± with D→KS0π+π− decays (2014)

A model-dependent amplitude analysis of B±→DK± with D→KS0π+π− decays is performed using proton–proton collision data, corresponding to an integrated luminosity of 1fb−1, recorded by LHCb at a centre-of-mass energy of 7 TeV in 2011. Values of the CP violation observables x± and y±, which are sensitive to the CKM angle γ, are measured to bex−=+0.027±0.044−0.008+0.010±0.001,y−=+0.013±0.048−0.007+0.009±0.003,x+=−0.084±0.045±0.009±0.005,y+=−0.032±0.048−0.009+0.010±0.008, where the first uncertainty is statistical, the second systematic and the third arises from the uncertainty of the D→KS0π+π− amplitude model. The value of γ is determined to be (84−42+49)°, including all sources of uncertainty. Neutral D meson mixing is found to have negligible effect.
Biosynthesis of murine terminal deoxynucleotidyltransferase. (1980)

An immunoprecipitation assay for measuring synthesis of murine terminal deoxynucleotidyltransferase (EC 2.7.7.31) has been developed using rabbit antiserum to calf terminal transferase. The antiserum precipitates a single Mr = 60,000 polypeptide (TdT-60) from all cell lines and tissues that contain enzymologically demonstrable terminal transferase. This polypeptide is not precipitated from labeled extracts of cells that lack terminal transferase by enzymological criteria. TdT-60 fractionates with terminal transferase during phosphocellulose chromatography and sediments with it in a sucrose gradient. TdT-60 is not detectably processed to lower molecular weight polypeptides, and terminal transferase activity sediments as a Mr = 60,000 activity; thus, we believe it to be the active form of terminal transferase. Using this assay we have demonstrated that terminal transferase is synthesized in both the murine thymus and the bone marrow at a rate proportional to its biochemically measured steady state level. After cortisone treatment of mice, the Mr = 60,000 polypeptide disappears from the thymus and then reappears as the thymus begins to be repopulated.
Ovarian Actions of Tumor Necrosis Factor-α (TNFα): Pleiotropic Effects of TNFα on Differentiated Functions of Untransformed Swine Granulosa Cells* (1991)

We have examined interactions between tumor necrosis factor-alpha (TNF alpha), a product of the immune system, and ovarian cells using serum-free monolayer cultures of untransformed swine granulosa cells. Recombinant human TNF alpha, a potent cytoactive product of activated macrophages, bound specifically and with high affinity to intact granulosa cells. Binding sites had an apparent Kd of 0.17 nM (95% confidence interval, 0.065-0.31), and a binding capacity of 80 nmol/micrograms DNA (95% confidence interval, 52-110). The binding capacity of granulosa cells for TNF alpha (but not the binding affinity) was increased approximately 2-fold by treatment with FSH and insulin. The biological effects of TNF alpha on pig granulosa cells were expressed after 48 and 96 h in culture. At the latter time, TNF alpha significantly suppressed insulin- and insulin- plus FSH-stimulated progesterone accumulation, with respective ID50 values of 0.08 +/- 0.008 and 0.06 +/- 0.014 nM, but did not affect basal progesterone accumulation or DNA content. TNF alpha also significantly attenuated the stimulatory effect of combined treatment with FSH and insulin on cAMP generation during 48-96 h of culture. TNF alpha inhibited the stimulatory effects of forskolin, cholera toxin, and the cAMP analog 8-bromo-cAMP on progesterone accumulation, indicating multiple sites of action of this immune modulator. Inhibition of progestin biosynthesis was observed even in the presence of 25-hydroxycholesterol, a soluble oxygenated sterol substrate for the cholesterol side-chain cleavage reaction, and was accompanied by decreased concentrations of specific cellular mRNA encoding cholesterol side-chain cleavage enzyme. There were no changes in the amounts of a constitutively expressed enzyme, phosphoglyceraldehyde dehydrogenase. Inhibitory actions of TNF alpha were specific to de novo steroid hormone biosynthesis, since nanomolar concentrations of this cytokine stimulated accumulation of prostaglandin E2 and prostaglandin F2 alpha basally and during treatment with FSH, cholera toxin, or 8-bromo-cAMP. In contrast, prostaglandin accumulation was not enhanced by interferon-gamma or interleukin-2. In summary, untransformed porcine granulosa cells exhibit specific, high affinity, low capacity saturable binding sites for TNF alpha, and the number of such binding sites can be regulated by combined treatment with insulin and FSH. Granulosa cells are susceptible to the inhibitory actions of TNF alpha on FSH- and insulin-supported progesterone biosynthesis and cAMP accumulation. One important locus of TNF alpha action is blockade of hormonally stimulated increases in specific mRNA encoding the cholesterol side-chain cleavage cytochrome P450 enzyme.(ABSTRACT TRUNCATED AT 400 WORDS)
Current collapse and reliability mechanisms in GaN HEMTs (2007)

Current collapse and performance degradation effects remain the most serious problem in short channel GaN HEMTs with operating frequencies above 20 GHz and aggressive designs for achieving highest power levels (exceeding 6-8 W/mm). Although for lower frequency operation, significant progress in mitigating these effects has been achieved, this approach is not applicable for ultra high frequency, higher performance devices. To facilitate the development of such advanced devices, the authors propose novel model applicable to short (down to sub-100 nm) channel devices operating in high power regime. This novel model accounts for three different degradation mechanisms related to (a) current collapse, (b) gate leakage current degradation and (c) structural damage caused by high electric fields.
Supramolecular Linear Heterojunction Composed of Graphite-Like Semiconducting Nanotubular Segments (2011)

One-dimensionally connected organic nanostructures with dissimilar semiconducting properties are expected to provide a reliable platform in understanding the behaviors of photocarriers, which are important for the development of efficient photon-to-electrical energy conversion systems. Although bottom-up supramolecular approaches are considered promising for the realization of such nanoscale heterojunctions, the dynamic nature of molecular assembly is problematic. We report a semiconducting nanoscale organic heterojunction, demonstrated by stepwise nanotubular coassembly of two strategically designed molecular graphenes. The dissimilar nanotubular segments, thus connected noncovalently, were electronically communicable with one another over the heterojunction interface and displayed characteristic excitation energy transfer and charge transport properties not present in a mixture of the corresponding homotropically assembled nanotubes.
Urinary sex-dependent low molecular weight proteins as a sign of narcotic dependence in male rats. (1983)

The relationship between urinary excretion of sex-dependent low molecular weight proteins (LMWP) in male rats and narcotic dependence has been investigated in this study. Chronic administration of codeine (0.5 mg/g food) caused a significant decrease in urinary excretion of LMWP from the third day, without any change in urinary high molecular weight proteins. The decrease recovered to the control level after the withdrawal of codeine. Withdrawal symptoms including loss of body weight and diarrhea were observed following codeine withdrawal. In animals chronically treated with pethidine (1.0 mg/g food), however, neither changes in urinary LMWP excretion nor withdrawal symptoms were observed. These present findings suggest that the decrease in urinary excretion of sex-dependent LMWP is related to narcotic dependence in male rats, since we previously reported the decrease in urinary excreted LMWP in morphine-dependent rats.
A cmos impulse radio ultra-wideband transceiver for 1Mb/s data communications and //sup +//sub -//2.5cm range findings (2005)

A CMOS impulse radio ultra-wideband (IR-UWB) transceiver is developed in 0.18/spl mu/m CMOS. It can be used for a 1Mb/s data transceiver, as well as a range finder within an error of /spl plusmn/2.5cm. Chip layout area of a transmitter and a receiver is 0.035mm/sup 2/ and 0.38mm/sup 2/, respectively. Power dissipation for 1Mb/s data communications is 0.7mW for the transmitter and 4.0mW for the receiver. Bit error rate is lower than 10/sup -5/ for ranges of shorter than 95cm, and 10/sup -3/ for 1m distance. For use of the range finder, power dissipation of the transmitter is 0.7/spl mu/W for 1000 measurements per second. Electronic field intensity is lower than 35/spl mu/V/m, in compliance with the regulation for the extremely low power radio station. Digital transmitter and clocked correlator are proposed for reduction in both layout area and power dissipation.
Prevention of Acute Lung Injury by a Novel CD14-Inhibitory Receptor Activator of the NF-κB Ligand Peptide in Mice (2021)

Although CD14 has been implicated in the initiation of multiple TLR-mediated inflammatory responses to sepsis and sepsis-related acute lung injury (ALI), an inhibitor of CD14, except for neutralizing Abs, has not been developed. A partial peptide, microglial healing peptide 1 with N-terminal acetylation and C-terminal amidation (MHP1-AcN), derived from the receptor activator of the NF-кB ligand, was recently found to inhibit multiple TLR signaling in the macrophages. Therefore, we hypothesized that the inhibitory effect of MHP1-AcN might be through the inhibition of CD14, a common coreceptor for multiple TLRs. In cultured mouse macrophages, MHP1-AcN was shown to bind to CD14 and compete with LPS for competitive inhibition of CD14, resulting in inhibition of TLR4 signaling, including NF-кB and IFN regulatory factor 3 activation and nuclear translocation. In addition to TLR2, TLR4, and TLR7, MHP1-AcN also inhibited TLR3 signaling and <i>Escherichia coli</i> DNA-induced, CD14-dependent TLR9 signals; however, CpG oligodeoxynucleotide-induced, CD14-independent TLR9 signals were not inhibited in the mouse macrophages. In sepsis-induced ALI mouse model, MHP1-AcN treatment showed the reduction in the expression of IL-6 and CCL2 in both the serum and lung tissues. IL-6 levels in the bronchoalveolar lavage fluid and pathological score were also decreased by MHP1-AcN. Thus, MHP1-AcN, a novel CD14 inhibitor, could be a promising agent for treating sepsis-induced ALI.
EPID-13. CHARACTERIZATION OF PEDIATRIC RADIATION-INDUCED MENINGIOMA: A SEER ANALYSIS (2022)

Abstract OBJECTIVE Radiation-induced meningiomas (RIM) are the most common secondary neoplasm arising from radiation therapy. RIM have not been characterized in pediatric populations on a national scale despite displaying shorter latency and higher malignancy rates in institutional studies. The objective of this study was to characterize pediatric RIM and compare latency and malignancy rates to those of adult RIM using the National Cancer Institute’s Surveillance, Epidemiology and End Results Program (SEER) database. METHODS From the SEER Research Plus Nov 2020 (1975-2018) dataset, 56161 patients treated with EBRT for ocular/orbital tumors, brain/nervous system tumors, and leukemia were identified. 185 patients meeting criteria for RIM were selected: cranial/spinal EBRT (presumed from diagnostic indication), histologically distinct precedent tumor treated with EBRT, and a minimum 5-year latency from EBRT to meningioma diagnosis. This sub-population was divided into 2 groups – pediatric (ages 0-19, n = 10) and adult (ages &amp;gt; 20, n = 175) RIM – and latency, recurrence rate and multiplicity were compared for each group. The number of tumors classified as malignant (as opposed to benign or borderline malignant) was also compared between groups. Additionally, demographic data were compared for each group. RESULTS Of the pediatric RIM patients, 70% were male, 70% were Caucasian, and 10% were African American. No pediatric RIM patients experienced a recurrence or presented with multiple RIMs. The pediatric group showed lower latency (11.00 ± 1.94 years, p &amp;lt; 0.001) and a higher malignancy rate (30%, p = 0.002) than the adult group (latency of 23.38 ± 8.69 years; malignancy rate 5.1%). CONCLUSION These data represent the first characterization of pediatric RIM from a national database and support prior findings of shorter latency and higher malignancy rates in pediatric vs adult RIM, as well as lay the groundwork for future nationwide monitoring of this rare yet highly significant complication.
Ligand‐Enabled β‐C(sp3)−H Lactamization of Tosyl‐Protected Aliphatic Amides Using a Practical Oxidant (2022)

The development of C(sp<sup>3</sup> )-H functionalization reactions that use common protecting groups and practical oxidants remains a significant challenge. Herein we report a monoprotected aminoethyl thioether (MPAThio) ligand-enabled β-C(sp<sup>3</sup> )-H lactamization of tosyl-protected aliphatic amides using tert-butyl hydrogen peroxide (TBHP) as the sole oxidant. This protocol features exceedingly mild reaction conditions, reliable scalability, and the use of practical oxidants and protecting groups. Further derivatization of the β-lactam products enables the synthesis of a range of biologically important motifs including β-amino acids, γ-amino alcohols, and azetidines.
A Battery Charge Controller for a Flyback Converter With Digital Primary Side Regulation (2013)

The market of electronic products such as mobile phones and portable devices grows rapidly while the demand of lithium battery for fast charge, long duration, and long life cycle increases significantly as well. There are several charge methods for lithium battery, such as constant current (CC)/constant voltage (CV) charge [1], pulse charge [2] and reflex charge [3], etc. In general, a flyback converter has been widely adopted as the front-end converter of battery charger due to its low-cost, wide power range, and galvanic isolation between input and output stage. The battery charge controller with flyback converter has been developed in digital control with opto-coupler feedback [4]–[6]. The conventional flyback converter with CC or CV applications always requires two sensed ADC channels for regulating [7]–[9]. An improved control method is proposed in this study to achieve CC/CV by only one ADC channel. This study presents a battery charge mechanism with a flyback converter and associated trickle charge method for the temperature compensation. The PSR is adopted for switching the charge modes within trickle current (TC), constant current (CC) and constant voltage (CV). The TC charging method is used to avoid the rapid increase in the temperature of the battery which is based on the characteristics of the internal resistance of the battery [10]. The proposed system circuit contains two stages in topology. The first stage is a front-end flyback converter which operates in DC-DC discontinuous current mode (DCM) by using PSR to sense the output voltage from auxiliary winding. It is adopted to replace the opto-coupler for avoiding temperature limitation for operations and reducing cost. The software Powersim is used to simulate the proposed flyback converter and PSR method. The second stage is the digital feedback loop, which processes the controlled signal by a digital processor (DSP) TMS320F2812. The experimental results were compared with general CC/CV method. The results show favorable performance of the propose charging method.