gebhart/patrepeval huggingface dataset:
Changes in endothelial actin cytoskeleton at leakage sites in the rat mesenteric microvasculature (1995)

The purpose of the present study was to compare the endothelial actin cytoskeleton at sites of inflammation-induced macromolecular leakage with that of intact endothelium. The circulation of a selected mesenteric window of anesthetized rats was perfused for 3 min with histamine (100 microM) plus fluorescein isothiocyanate (FITC)-albumin, or FITC-albumin alone, and was fixed at physiological pressure. The vasculature was then perfusion stained with rhodamine phalloidin to label filamentous actin (F-actin) and examined with a confocal microscope. The leakage sites were divided into three categories based on the extent of FITC-albumin leakage: 1) focal leaks, 2) mid leaks, and 3) extended leaks. The leaks were identifiable with a particular endothelial cell(s), and the structure of the endothelial actin cytoskeleton was characterized at these sites. Focal leaks occurred along a small region of endothelial cell-cell contact and in most observed cases were located at a region of specific disruption of the endothelial peripheral actin rim (PAR). Mid leaks involved the disruption of one endothelial cell, and the affected cell was observed to have extended disruption of the PAR as well as increased diffuse F-actin staining throughout the cell (1.9-fold increase relative to adjacent cells). Extended leaks involved the disruption of two or more adjacent endothelial cells, and each cell exhibited an actin pattern similar to that seen at mid leaks. These results show that histamine-induced macromolecular leakage in situ is associated with significant changes in the endothelial actin cytoskeleton.
High-performance MSM photodetectors using Cu Schottky contacts (1997)

GaInAs metal-semiconductor-metal photodetectors fabricated with Cu Schottky contacts on an AlInAs barrier-enhancement layer are described. The Cu electrodes were found to decrease by a factor of two the dark current of large area pads compared to pads formed with Ti, illustrating the potential for improving Schottky barriers by proper choice of metal type. The dark current density at 10 V is 3.8 pA/μm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> and the response time is 17 ps.
Crucial involvement of the CCL3‐CCR5 axis‐mediated fibroblast accumulation in colitis‐associated carcinogenesis in mice (2014)

Patients with inflammatory bowel diseases often develop colon carcinoma. Combined treatment of azoxymethane (AOM) and dextran sulfate sodium (DSS) recapitulates colitis‐associated cancer in mice. AOM/DSS‐induced tumor formation was reduced in CCL3 ‐ or its specific receptor, CCR5 ‐deficient mice despite the presence of a massive infiltration of inflammatory cells. However, AOM/DSS‐induced type I collagen‐positive fibroblast accumulation in the colon was reduced in CCL3‐ or CCR5 ‐deficient mice. This was associated with depressed expression of heparin‐binding epidermal growth factor‐like growth factor (HB‐EGF), which is expressed mainly by fibroblasts. Moreover in vitro, CCL3 induced fibroblasts to proliferate and to enhance HB‐EGF expression. Furthermore, CCR5 blockade reduced tumor formation together with reduced fibroblast accumulation and HB‐EGF expression, even when administered after the development of multiple colon tumors. Thus, CCL3‐CCR5‐mediated fibroblast accumulation may be required, in addition to leukocyte infiltration, to induce full‐blown colitis‐associated carcinogenesis. Our studies shed light on a therapeutic potential of CCR5 antagonist for patients with colitis‐associated cancer.
Xilinx First 7nm Device: Versal AI Core (VC1902) (2019)

This article consists of a collection of slides from the author's conference presentation.
Electron-transfer protein reactivities. Kinetic studies of the oxidation of horse heart cytochrome c, Chromatium vinosum high potential iron-sulfur protein, Pseudomonas aeruginosa azurin, bean plastocyanin, and Rhus vernicifera stellacyanin by pentaammminepyridineruthenium(III) (1977)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectron-transfer protein reactivities. Kinetic studies of the oxidation of horse heart cytochrome c, Chromatium vinosum high potential iron-sulfur protein, Pseudomonas aeruginosa azurin, bean plastocyanin, and Rhus vernicifera stellacyanin by pentaammminepyridineruthenium(III)Diane Cummins and Harry B. GrayCite this: J. Am. Chem. Soc. 1977, 99, 15, 5158–5167Publication Date (Print):June 1, 1977Publication History Published online1 May 2002Published inissue 1 June 1977https://pubs.acs.org/doi/10.1021/ja00457a042https://doi.org/10.1021/ja00457a042research-articleACS PublicationsRequest reuse permissionsArticle Views322Altmetric-Citations84LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
RELATIONSHIP BETWEEN CLINIC WAITING TIME AND SATISFACTION AMONG RHEUMATOID ARTHRITIS PATIENTS (2013)

Objectives: Long clinic waiting time is frequently claimed to be a major cause of patient dissatisfaction, but how the two are connected is not clearly understood. According to Oliver`s expectancy disconfirmation theory, the feeling of dissatisfaction is generally a result of reality falling short of expectations. Therefore, we hypothesized that a patient`s dissatisfaction is in part a result of disconfirmation of his/her expected waiting time. This study aims to test this hypothesis. Methods: A telephone survey of all Rheumatoid Arthritis patients on follow-up at Singapore General Hospital was conducted between November 2011 and February 2012. Patients were asked to rate their overall satisfaction with care and satisfaction with professional emotional support respectively using a 0-10 scale. They were also asked to report their average perceived waiting time (PWT) in the 12 months prior to the survey, and their socio-demographic and clinical characteristics. Actual waiting time (AWT) during the same period for each patient was obtained from the hospital administrative database. Expectancy was considered to be disconfirmed if the average PWT was longer than the average AWT. Factors significantly associated with a satisfaction score in a bivariate analysis were entered into the corresponding multivariate linear regression analysis. Satisfaction with care and satisfaction with emotional support were analyzed separately. Results: Our final sample consisted of 552 patients (response rate 71.7%, mean(SD) age 57.85(11.99) years, 85% females, 76.8% Chinese). The median PWT and AWT were 133 and 95 minutes respectively (interquartile ranges: 198 & 114 minutes). Disconfirmed expectancy about waiting time was associated with on average 4.4% (p=.022) drop in satisfaction with professional emotional support, but it had no significant effect on overall satisfaction with care. As such, satisfaction with professional emotional support may be a more appropriate outcome measure than overall satisfaction with care when evaluating the effectiveness of efforts to shorten clinic waiting time.
Cardiac MR elastography for quantitative assessment of elevated myocardial stiffness in cardiac amyloidosis (2017)

2 Technical Efficacy: Stage 2 J. Magn. Reson. Imaging 2017;46:1361-1367.
Molecular Recognition: Comparative Study of a Tunable Host–Guest System by Using a Fluorescent Model System and Collision‐Induced Dissociation Mass Spectrometry on Dendrimers (2005)

Abstract Host–guest interactions between the periphery of adamantylurea‐functionalized dendrimers (host) and ureido acetic acid derivatives (guest) were shown to be specific, strong and spatially well‐defined. The binding becomes stronger when using phosphonic or sulfonic acid derivatives. In the present work we have quantified the binding constants for the host–guest interactions between two different host motifs and six different guest molecules. The host molecules, which resemble the periphery of a poly(propylene imine) dendrimer, have been fitted with an anthracene‐based fluorescent probe. The two host motifs differ in terms of the length of the spacer between a tertiary amine and two ureido functionalities. The guest molecules all contain an acidic moiety (either a carboxylic acid, a phosphonic acid, or a sulfonic acid) and three of them also contain an ureido moiety capable of forming multiple hydrogen bonds to the hosts. The binding constants for all 12 host–guest complexes have been determined by using fluorescence titrations by monitoring the increase in fluorescence of the host upon protonation by the addition of the guest. The binding constants could be tuned by changing the design of the acidic part of the guest. The formation of hydrogen bonds gives, in all cases, higher association constants, demonstrating that the host is more than a proton sensor. The host with the longer spacer (propyl) shows higher association constants than the host with the shorter spacer (ethyl). The gain in association constants are higher when the urea function is added to the guests for the host with the longer spacer, indicating a better fit. Collision‐induced dissociation mass spectrometry (CID‐MS) is used to study the stability of the six motifs using the corresponding third generation dendrimer. A similar trend is found when the six different guests are compared.
Late Development of Vitelliform Lesions and Flecks in a Patient With Best Disease (2005)

Findings characteristic of Best disease may not manifest in a molecularly affected individual until late in life. Mutations in bestrophin appear to lead to extracellular deposit formation outside the macula in some families. The distribution of bestrophin in the RPE suggests that the protein may be mistargeted in those with Best disease who have the Y227N mutation, and that this may be a cause of the associated RPE and photoreceptor dysfunction.
Actin-Based Molecular Motors for Cargo Transportation in Nanotechnology&#8212; Potentials and Challenges (2005)

Here, we review the use of actin-based motors, (myosins; e.g., the molecular motor of muscle) in nanotechnology. The review starts from the viewpoints of the molecular motors as being important devices responsible of cargo transportation in the cell and end in discussions about their employment in nanotechnological applications. First, we describe basic biophysics of the myosin motors with focus on their involvement in cargo transportation in the living cell, leading us over into a discussion about in vitro motility assays. These are biological test systems where the myosin-induced translocation of actin filaments is studied on an artificial surface outside the cell. Then follows a review about modified motility assays for production of ordered motion. Here, we discuss ours and others' work with regards to making micro- and nanostructured surfaces and channels where the position and direction of movement produced by molecular motors is controlled. In this section, we consider the role of the channel size in promoting unidirectional myosin-induced motion of actin filaments. Furthermore, we consider the usefulness of surface modifications, e.g., various silanization procedures in order to promote and hinder molecular motility, respectively. Particularly, we describe our latest test system being both morphologically and chemically nanostructured giving us unsurpassed possibilities to perform functional studies as well as extremely good spatio-temporal control. Then follows a section about nanotechnological cargo transportation systems based on the actomyosin motor system. For instance, we present results of attaching fluorescent quantum dots as cargoes to the actin filaments. In this section, we also discuss the possibilities of having cargo attachment and detachment being performed on demand. Finally, we consider the usefulness of molecular motors for lab-on-a-chip applications and the requirements for incorporating these motors in commercially viable devices. In this context, the significant potential of the actomyosin motor system to overcome traditional limitations of micro- and nanofluidics is stressed.
Elucidating the Interaction of CF Airway Epithelial Cells and Rhinovirus: Using the Host-Pathogen Relationship to Identify Future Therapeutic Strategies (2018)

Chronic lung disease remains the primary cause of mortality in cystic fibrosis (CF). Growing evidence suggests respiratory viral infections are often more severe in CF compared to healthy peers and contributes to pulmonary exacerbations (PEx) and deterioration of lung function. Rhinovirus is the most prevalent respiratory virus detected, particularly during exacerbations in children with CF <5 years old. However, even though rhinoviral infections are likely to be one of the factors initiating the onset of CF lung disease, there is no effective targeted treatment. A better understanding of the innate immune responses by CF airway epithelial cells, the primary site of infection for viruses, is needed to identify why viral infections are more severe in CF. The aim of this review is to present the clinical impact of virus infection in both young children and adults with CF, focusing on rhinovirus infection. Previous <i>in vitro</i> and <i>in vivo</i> investigations looking at the mechanisms behind virus infection will also be summarized. The review will finish on the potential of transcriptomics to elucidate the host-pathogen responses by CF airway cells to viral infection and identify novel therapeutic targets.
Nonincremental coherent optical fiber sensor (1988)

A novel coherent optical fiber sensor for nonincremental measurements of phase-conveyed information is reported. When the frequency of a laser source is sinusoidally modulated, a nonincremental measurement can be processed with the same frequency-demodulation treatment as used for an incremental one. This method is applied to a polarimetric fiber-optic sensor to obtain the absolute temperature measurement in the range of 20-100 degrees C. A rotating half-wave plate gives the polarimetric setup the frequency carrier essential for the demodulation process. Experimental data confirming the theoretical predictions are reported together with the measured sensitivity values.
Supernarrowing mirrorless laser emission in dendrimer-doped polymer waveguides (2000)

A supernarrowing laser spectrum is observed for mirrorless polymer waveguide lasers containing rhodamine B cored dendrimers. The moderate scattering from the aggregated dendrimers in the host polymer matrix is responsible for the mirrorless lasing. However, the long amplification path through the waveguide produces a directional beam and supernarrowing a spectrum in scattering lasers.
DreamSketch (2017)

We present DreamSketch, a novel 3D design interface that combines the free-form and expressive qualities of sketching with the computational power of generative design algorithms. In DreamSketch, a user coarsely defines the problem by sketching the design context. Then, a generative design algorithm produces multiple solutions that are augmented as 3D objects in the sketched context. The user can interact with the scene to navigate through the generated solutions. The combination of sketching and generative algorithms enables designers to explore multiple ideas and make better informed design decisions during the early stages of design. Design study sessions with designers and mechanical engineers demonstrate the expressive nature and creative possibilities of DreamSketch.
Development and Validation of Globally Asymptotically Stable Control Laws for Automatic Tractor Guidance (2011)

Automatic guidance of agricultural vehicles requires the use of sensors and control techniques. While the Global Position System (GPS) is widely used as a position sensor, different control laws have been used in the scientific literature based on geometry, heuristics, and models. In this article, two new control laws for navigation control of agricultural tractors were developed with one for following straight lines and the other for tracking circular arcs. These two controllers have virtually the same structure based on the tractor's kinematic model. They have no singularity point in the state space. Also, global asymptotic stability is guaranteed with the control laws. Guidance with the proposed controllers in straight lines and circular arc trajectories was simulated and field-tested. The experiments were performed with a tractor equipped with an automatic GPS guidance system. In both simulation and experiment, the control laws led the tractor to converge to the desired trajectories of straight lines and circular arc paths under three different low speeds of 1, 2, and 3 m/s and four different initial conditions that are 90 apart. The simulation and experimental results show the practical validity of the proposed controllers.
Abstract 33: Combination of Allogeneic Mesenchymal and Kidney-derived Stem Cells Promotes Kidney Repair in a Swine Model of Chronic Kidney Disease (2017)

Background: Chronic kidney disease (CKD) has a high prevalence (~14% of the population) and is associated with a significantly increased risk of cardiovascular disease and a 15-fold higher rate of mortality than the general population. Current therapies slow disease progression but do not repair organ damage, leading to end-stage renal disease. Stem cell therapy has the potential to promote repair via neovascularization and antifibrotic effects. We tested the renal reparative capacity of allogeneic mesenchymal stem cells (MSCs) and kidney ckit+ stem cells (c-kit) in an established swine model of CKD. Methods: Yorkshires pigs (n=27) underwent 5/6 nephrectomy via renal artery embolization and 4-weeks later received either: MSC (10х10 6 ), c-kit (10х10 6 ), combination (MSC+c-kit; 1:1 ratio [5х10 6 each]), or placebo (each n=5). Allogeneic cell therapy was delivered via the patent renal artery of the remnant kidney. Kidney functional parameters and renal MRI were measured at baseline, and at 4- and 12-weeks (euthanasia) post-embolization. Results: The CKD model was validated from baseline to 4 weeks by an increased creatinine: (Δ1.1 ± 0.15 mg/dl; p&lt;0.0001), BUN (Δ13.50 ± 2.99mg/dl; p=0.0003), and urine protein/creatinine ratio (Δ0.311mg/g; p=0.018), and decreased GFR (Δ49.82 ±6.41 ml/min; p=0.0002). Mean arterial pressure (MAP) was not different between groups from baseline to 4 weeks. After 12 weeks, there was a significant difference in MAP between groups (p=0.04), with an increase in the placebo group (19.97± 8.65 mmHg, p=0.08). BUN and creatinine levels improved in all of the groups from 4-12 weeks. GFR also improved in all the groups, but with the greatest effect in the combination group (76± 23.83ml/min; p= 0.03) from 4-12 weeks. Urine protein/creatinine ratio did not change in placebo but decreased in cell treated groups. There was no evidence of immune rejection as evaluated in a complete body necropsy. Conclusion: Allogeneic MSCs and kidney-derived stem cells are safe in a CKD swine model. The combination of stem cells was shown to be more efficacious in improving kidney function. These novel findings have important implications for the advancement of cell therapy for CKD.
Sunlight‐Triggerable Transient Energy Harvester and Sensors Based on Triboelectric Nanogenerator Using Acid‐Sensitive Poly(phthalaldehyde) (2019)

Abstract Transient electronics that disintegrate via material dissolution or depolymerization under certain stimuli have great potential in biomedical and military applications. The triboelectric nanogenerator (TENG), an emerging mechanical energy harvesting technology with great flexibility in material choices, is promising in offering transient power sources. Previously reported transient energy harvesters using biodegradable polymers require solution‐based degradation and have limited applications in non‐biological scenarios. A short time span, sunlight‐triggered degradable TENG is developed. The main substrate includes an acid‐sensitive poly(phthalaldehyde) (PPHA), a photoacid generator (PAG), and a photosensitizer (PS). Through photo‐induced electron transfer, the ultraviolet radiation absorbed by the PS is transferred to the PAG to generate photoacids that trigger the depolymerization of PPHA. Transient TENG‐based mechanical energy harvesters and touch/acoustic sensors are successfully demonstrated by embedding silver nanowires onto the PPHA‐based films. The fabricated devices degrade rapidly under winter sunlight. The degradation rate can be further tuned via changing the ratio of photosensitive agents. This work not only broadens the applicability of TENG as transient power sources and sensors, but also extends the use of transient functional polymers toward advanced energy and sensing applications.
ChemInform Abstract: Microwave‐Assisted Transamidation of Ureas. (2016)

Abstract A novel one‐step synthesis of ureas via the transamidation of the cyclopentyl or isopropyl ureas with excess amines under microwave irradiation is reported.
Evaluation of the Orbitrap Ascend Tribrid Mass Spectrometer for Shotgun Proteomics (2023)

Mass spectrometry (MS)-based proteomics is a powerful technology to globally profile protein abundances, activities, interactions, and modifications. The extreme complexity of proteomics samples, which often contain hundreds of thousands of analytes, necessitates continuous development of MS techniques and instrumentation to improve speed, sensitivity, precision, and accuracy, among other analytical characteristics. Here, we systematically evaluated the Orbitrap Ascend Tribrid mass spectrometer in the context of shotgun proteomics, and we compared its performance to that of the previous generation of Tribrid instruments─the Orbitrap Eclipse. The updated architecture of the Orbitrap Ascend includes a second ion-routing multipole (IRM) in front of the redesigned C-trap/Orbitrap and a new ion funnel that allows gentler ion introduction, among other changes. These modifications in Ascend hardware configuration enabled an increase in parallelizable ion injection time during higher-energy collisional dissociation (HCD) Orbitrap tandem MS (FTMS<sup>2</sup>) analysis of ∼5 ms. This enhancement was particularly valuable in the analyses of limited sample amounts, where improvements in sensitivity resulted in up to 140% increase in the number of identified tryptic peptides. Further, analysis of phosphorylated peptides enriched from the K562 human cell line yielded up to ∼50% increase in the number of unique phosphopeptides and localized phosphosites. Strikingly, we also observed a ∼2-fold boost in the number of detected N-glycopeptides, likely owing to the improvements in ion transmission and sensitivity. In addition, we performed the multiplexed quantitative proteomics analyses of TMT11-plex labeled HEK293T tryptic peptides and observed 9-14% increase in the number of quantified peptides. In conclusion, the Orbitrap Ascend consistently outperformed its predecessor the Orbitrap Eclipse in various bottom-up proteomic analyses, and we anticipate that it will generate reproducible and in-depth datasets for numerous proteomic applications.
An Extensible Markup Language (XML) Configuration Access Protocol (XCAP) Usage for Manipulating Presence Document Contents (2007)

community, and requests discussion and suggestions for improvements.