gebhart/patrepeval huggingface dataset:
Cellobiose Dehydrogenase from the Ligninolytic Basidiomycete Ceriporiopsis subvermispora (2009)

Cellobiose dehydrogenase (CDH), an extracellular flavocytochrome produced by several wood-degrading fungi, was detected in cultures of the selective delignifier Ceriporiopsis subvermispora when grown on a cellulose- and yeast extract-based liquid medium. CDH amounted to up to 2.5% of total extracellular protein during latter phases of the cultivation and thus suggested an important function for the fungus under the given conditions. The enzyme was purified 44-fold to apparent homogeneity. It was found to be present in two glycoforms of 98 kDa and 87 kDa with carbohydrate contents of 16 and 4%, respectively. The isoelectric point of both glycoforms is around 3.0, differing by 0.1 units, which is the most acidic value so far reported for a CDH. By using degenerated primers of known CDH sequences, one cdh gene was found in the genomic DNA, cloned, and sequenced. Alignment of the 774-amino-acid protein sequence revealed a high similarity to CDH from other white rot fungi. One notable difference was found in the longer interdomain peptide linker, which might affect the interdomain electron transfer at higher temperatures. The preferred substrate of C. subvermispora CDH is cellobiose, while glucose conversion is strongly discriminated by a 155,000-fold-lower catalytic efficiency. This is a typical feature of a basidiomycete CDH, as are the acidic pH optima for all tested electron acceptors in the range from 2.5 to 4.5.
Self-Sensing via Flux Injection With Rapid Servo Dynamics Including a Smooth Transition to Back-EMF Tracking Self-Sensing (2020)

Self-sensing control at zero and low speed has been widely implemented and studied by many researchers. This technique is based on injecting a high-frequency voltage or current into the motor to estimate the motor position and speed from the measured motor quantities like current or voltage. Tracking the motor back-EMF or flux signal has been used to control motors during medium- and high-speed operations. This article proposes a high-frequency flux injection (HFFI) based self-sensing using deadbeat-direct torque and flux control to control permanent magnet servo motor at zero and low speed with ideally zero torque ripple, unlike the standard current or voltage injection techniques. The HFFI is combined with a back EMF self-sensing technique to control the motor during medium- and high-speed operations. This article also proposes a method for a smooth transition between these two methods. The proposed controller is used to control different types of slightly salient servo motors during dynamic servo operations.
A Multimodal Assessment of the Genetic Control over Working Memory (2010)

Working memory performance is significantly influenced by genetic factors. Here, we assessed genetic contributions to both working memory performance and neuroimaging measures focused on the network of brain regions associated with working memory by using a sample of 467 human participants from extended families. Imaging measures included diffusion tensor imaging indices in major white matter tracts thought to be associated with working memory and structural magnetic resonance imaging measures of frontal and parietal gray matter density. Analyses directly addressed whether working memory performance and neural structural integrity are influenced by common genetic factors (e.g., pleiotropy). While all cognitive measures, gray matter regions, and white matter tracts assessed were heritable, only performance on a spatial delayed response task and integrity of the superior longitudinal fasciculus (a primary fronto-parietal connection) shared genetic factors. As working memory may be a core component of other higher level processes, such as general intelligence, this finding has implications for the heritability of complex cognitive functions, as well as for our understanding of the transmission of cognitive deficits in mental and neurological disorders.
Highly Efficient Blue‐Emitting Cyclometalated Platinum(II) Complexes by Judicious Molecular Design (2013)

Tiefblaue Strahler auf der Grundlage von cyclometallierten Platin(II)-Komplexen wurden synthetisiert, charakterisiert und in organischen Leuchtdioden eingesetzt. Die Komplexe mit vierzähnigen Liganden verfügten über bessere photophysikalische Eigenschaften als ihre Iridiumanaloga, und eine Verbindung erreichte eine höchste externe Quanteneffizienz (EQE) von 23.7 %. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Origin of extra capacity in the solid electrolyte interphase near high-capacity iron carbide anodes for Li ion batteries (2020)

Both organic and inorganic ingredients in SEI undergo reversible conversions and contribute capacity enhancement with the catalysis of Fe<sub>3</sub>C.
Optimization of an Electrically-Heated Catalytic Converter System Calculations and Application (1993)

&lt;div class="htmlview paragraph"&gt;From SAE-Papers and several publications, the easy, effective function and management of an EHC-System is well known. The direction of the development is now to reduce the electrical energy consumption and to show the mechanical durability of the heating structure. This paper shows that it is possible to minimize the energy consumption and that the required service life can be in principle achieved with the introduction of these developments.&lt;/div&gt; &lt;div class="htmlview paragraph"&gt;The physical characteristics such as mass, geometrical surface area, cell density and electrical resistance of the EHC construction could be optimized to save energy. This, in conjunction with the operating parameters of the engine, the controlling of the secondary air and the catalyst configuration, will enable the goals to be met. The design of the converter, the physical characteristics and the results of the tests are shown with the Porsche 944 S2 and 968 applications. To further understand thermomechanical durability behavior, thermo-cycling, vibration analysis and high mileage durability tests on vehicle were carried out.&lt;/div&gt;
Increased efficiency of luminescent solar concentrators after application of organic wavelength selective mirrors (2012)

Organic wavelength-selective mirrors are used to reduce the loss of emitted photons through the surface of a luminescent solar concentrator (LSC). A theoretical calculation suggests that application of a 400 nm broad reflector on top of an LSC containing BASF Lumogen Red 305 as a luminophore can reflect 91% of all surface emitted photons back into the device. Used in this way, such broad reflectors could increase the edge-emission efficiency of the LSC by up to 66%. Similarly, 175 nm broad reflectors could increase efficiency up to 45%. Measurements demonstrate more limited effectiveness and dependency on the peak absorbance of the LSC. At higher absorbance, the increased number of internal re-absorption events reduces the effectiveness of the reflectors, leading to a maximum increase in LSC efficiency of ~5% for an LSC with a peak absorbance of 1. Reducing re-absorption by reducing dye concentration or the coverage of the luminophore coating results in an increase in LSC efficiency of up to 30% and 27%, respectively.
Interference Analysis for mm-Wave Picocells (2015)

Millimeter (mm) wave picocellular networks are a promising approach for delivering the 1000-fold capacity increase required to keep up with projected demand for wireless data: the available bandwidth is orders of magnitude larger than that in existing cellular systems, and the small carrier wavelength enables the realization of highly directive antenna arrays in compact form factor, thus drastically increasing spatial reuse. In this paper, we carry out an interference analysis for mm wave picocells in an urban canyon, accounting for the geometry associated with the sparse multipath characteristic of this band. While we make some modeling simplifications, our analysis provides a strong indication of the very large capacity, of the order of Terabits/sec per km, provided by such networks, using system bandwidths of the order of a few GHz.
The effect of substrate bias on hot-carrier damage in NMOS devices (1989)

Hot-carrier stressing carried out as a function of substrate voltage on 2- mu m NMOS devices under bias conditions V/sub d/=8 V and V/sub g/=5.5 V is discussed. The time power-law dependence of stressing changes as a function of substrate bias (V/sub b/), having a power-law gradient of 0.5 for V/sub b/=0 V and 0.3 for V/sub b/=-9 V. Investigation of the type of damage resulting from stressing shows that at V/sub b/=0 V, interface state generation results, while at V/sub b/=-9 V, the damage is mostly by charge trapping. Measurements of the gate current under these two substrate bias conditions show that the gate electron current increases by over two orders of magnitude upon application of a strong back bias. It is suggested that the electron trapping arises from this enhanced gate electron current under large substrate voltage conditions.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
DMD-based random-access optical-resolution photoacoustic microscopy (2014)

The scanning mechanism is a major technical focus in optical-resolution photoacoustic microscopy. Flexible scanning access with fast scanning speed is desired to monitor biological and physiological dynamics with high temporal resolution. We developed random-access optical-resolution photoacoustic microscopy (RA-OR-PAM) using a digital micromirror device (DMD). Each micromirror on the DMD can be independently controlled, allowing imaging of regions of interest with arbitrary user-selected shapes without extraneous information. A global structural image is first acquired, and the regions of interest are selected. The laser beam then scans these regions exclusively, resulting in a faster frame rate than in a conventional raster scan. This system can rapidly scan arbitrarily shaped regions of interest with a lateral resolution of 3.6 &mu;m within a 40&times;40 &mu;m<sup>2</sup> imaging area, a size comparable to the focal spot size of a 50 MHz ultrasound transducer. We demonstrated the random-access ability of RA-OR-PAM by imaging a monolayer of red blood cells. This system was then used to monitor blood flow in vivo within user-selected capillaries in a mouse ear. By imaging only the capillary of interest, the frame rate was increased by up to 13.3 times.
CFTR-deficient pigs display peripheral nervous system defects at birth (2013)

Peripheral nervous system abnormalities, including neuropathy, have been reported in people with cystic fibrosis. These abnormalities have largely been attributed to secondary manifestations of the disease. We tested the hypothesis that disruption of the cystic fibrosis transmembrane conductance regulator (CFTR) gene directly influences nervous system function by studying newborn CFTR(-/-) pigs. We discovered CFTR expression and activity in Schwann cells, and loss of CFTR caused ultrastructural myelin sheath abnormalities similar to those in known neuropathies. Consistent with neuropathic changes, we found increased transcripts for myelin protein zero, a gene that, when mutated, can cause axonal and/or demyelinating neuropathy. In addition, axon density was reduced and conduction velocities of the trigeminal and sciatic nerves were decreased. Moreover, in vivo auditory brainstem evoked potentials revealed delayed conduction of the vestibulocochlear nerve. Our data suggest that loss of CFTR directly alters Schwann cell function and that some nervous system defects in people with cystic fibrosis are likely primary.
A green route to polyurethanes: oxidative carbonylation of industrially relevant aromatic diamines by CO2-based methyl formate (2020)

Toluene-2,4-diamine, 4,4′-methylenedianiline and 2,4-diaminomesitylene were converted directly into the corresponding dicarbamates<italic>via</italic>the CO<sub>2</sub>-based intermediate methyl formate (MF) which can be regarded as a liquid source for CO/MeOH mixtures.
Robotics-driven manufacturing of cartilaginous microtissues for the bio-assembly of skeletal implants (2023)

Abstract Automated technologies are attractive for enhancing a robust manufacturing of tissue engineered products for clinical translation. In this work, we present an automation strategy using a robotics platform for media changes of cartilaginous microtissues cultured in static microwell platforms. We use an automated image analysis pipeline to extract microtissue displacements and morphological features, which serve as input for statistical factor analysis. To minimize microtissue displacement and suspension leading to uncontrolled fusion, we performed a mixed factorial DoE on liquid handling parameters for large and small microwell platforms. As a result, 144 images, with 51 471 spheroids could be processed automatically. The automated imaging workflow takes 2 minutes per image, and it can be implemented for on-line monitoring of microtissues, thus allowing informed decision making during manufacturing. We found that time in culture is the main factor for microtissue displacements, explaining 10 % of the displacements. Aspiration and dispension speed were not significant at manual speeds or beyond, with an effect size of 1 %. We defined optimal needle placement and depth for automated media changes and we suggest that robotic plate handling could improve the yield and homogeneity in size of microtissue cultures. After three weeks culture, increased expression of COL2A1 confirmed chondrogenic differentiation and RUNX2 shows no osteogenic specification. Histological analysis showed the secretion of cartilaginous extracellular matrix. Furthermore, microtissue-based implants were capable of forming mineralized tissues and bone after four weeks of ectopic implantation in nude mice. We demonstrate the development of an integrated bioprocess for culturing and manipulation of cartilaginous microtissues. We anticipate the progressive substitution of manual operations with automated solutions for manufacturing of microtissue-based living implants.
Longitudinal, Noninvasive Imaging of T-Cell Effector Function and Proliferation in Living Subjects (2010)

Adoptive immunotherapy is evolving to assume an increasing role in treating cancer. Most imaging studies in adoptive immunotherapy to date have focused primarily on locating tumor-specific T cells rather than understanding their effector functions. In this study, we report the development of a noninvasive imaging strategy to monitor T-cell activation in living subjects by linking a reporter gene to the Granzyme B promoter (pGB), whose transcriptional activity is known to increase during T-cell activation. Because pGB is relatively weak and does not lead to sufficient reporter gene expression for noninvasive imaging, we specifically employed 2 signal amplification strategies, namely the Two Step Transcription Amplification (TSTA) strategy and the cytomegalovirus enhancer (CMVe) strategy, to maximize firefly luciferase reporter gene expression. Although both amplification strategies were capable of increasing pGB activity in activated primary murine splenocytes, only the level of bioluminescence activity achieved with the CMVe strategy was adequate for noninvasive imaging in mice. Using T cells transduced with a reporter vector containing the hybrid pGB-CMVe promoter, we were able to optically image T-cell effector function longitudinally in response to tumor antigens in living mice. This methodology has the potential to accelerate the study of adoptive immunotherapy in preclinical cancer models.
Breast cancer in a male-to-female transsexual patient with a BRCA2 mutation (2016)

Breast cancer is rare in male patients. Certain predisposing factors, be they genetic (e.g., BRCA2 gene mutations) or hormonal (imbalance between estrogen and androgen levels), have been implicated in male breast cancer pathophysiology. Male-to-female (MtF) transsexualism is a condition that generally involves cross-sex hormone therapy. Anti-androgens and estrogens are used to mimic the female hormonal environment and induce the cross-sex secondary characteristics. In certain situations, the change in the hormonal milieu can be disadvantageous and favor the development of hormone-dependent pathologies, such as cancer. We report a case of a MtF transgender patient who developed breast cancer after 7 years of cross-sex hormonal therapy. The patient was found to be BRCA2 positive, and suffered recurrent disease. The patient was unaware of being a member of an established BRCA2 mutation-positive kindred. This represents the first case of a BRCA2 mutation predisposing to breast cancer in a MtF transgender patient.
Dijet Azimuthal Decorrelations in p p Collisions at s = 7 TeV (2011)

Artículo escrito por un elevado número de autores, solo se referencian el que aparece en primer lugar, el nombre del grupo de colaboración, si le hubiere, y los autores pertenecientes a la UAM
Custom Tokenization Dictionary, CUSTODI: A General, Fast, and Reversible Data-Driven Representation and Regressor (2021)

Custom tokenization dictionary (CUSTODI) is introduced as a novel way for tackling the problem of molecular representations, and especially the challenge of molecular property prediction. Herein, the motivational theory and the actual representation and model are presented and shown to have performance that is in line with benchmark methodologies. The uniqueness of CUSTODI is its applicability on small training sets and the developed theory suggests its possible use for a-priori estimation of future fit quality on any given dataset, regardless of the method used for fitting.
Anomalous interface fixed charge generated by forming gas annealing in SiO2/GaN MOS devices (2020)

We investigated the effect of forming gas annealing (FGA) on the electrical properties of SiO2/GaN MOS devices. We conducted systematic capacitance–voltage measurements on metal-oxide-semiconductor structures and observed a significant amount of anomalous positive fixed charge (3 × 1012 cm−2) at the interface between SiO2 and GaN for FGA temperatures over 250 °C. The fixed charges generated by FGA may be attributed to oxygen vacancies formed in the gallium oxide (GaOx) interlayer by the reaction between GaOx and hydrogen.
An antennal carboxylesterase from Drosophila melanogaster, esterase 6, is a candidate odorant-degrading enzyme toward food odorants (2015)

Reception of odorant molecules within insect olfactory organs involves several sequential steps, including their transport through the sensillar lymph, interaction with the respective sensory receptors, and subsequent inactivation. Odorant-degrading enzymes (ODEs) putatively play a role in signal dynamics by rapid degradation of odorants in the vicinity of the receptors, but this hypothesis is mainly supported by in vitro results. We have recently shown that an extracellular carboxylesterase, esterase-6 (EST-6), is involved in the physiological and behavioral dynamics of the response of Drosophila melanogaster to its volatile pheromone ester, cis-vaccenyl acetate. However, as the expression pattern of the Est-6 gene in the antennae is not restricted to the pheromone responding sensilla, we tested here if EST-6 could play a broader function in the antennae. We found that recombinant EST-6 is able to efficiently hydrolyse several volatile esters that would be emitted by its natural food in vitro. Electrophysiological comparisons of mutant Est-6 null flies and a control strain (on the same genetic background) showed that the dynamics of the antennal response to these compounds is influenced by EST-6, with the antennae of the null mutants showing prolonged activity in response to them. Antennal responses to the strongest odorant, pentyl acetate, were then studied in more detail, showing that the repolarization dynamics were modified even at low doses but without modification of the detection threshold. Behavioral choice experiments with pentyl acetate also showed differences between genotypes; attraction to this compound was observed at a lower dose among the null than control flies. As EST-6 is able to degrade various bioactive odorants emitted by food and plays a role in the response to these compounds, we hypothesize a role as an ODE for this enzyme toward food volatiles.
Abstract #1882: A gene expression signature associated with \#8220;K-Ras addiction\#8221; reveals novel candidate therapeutic targets that regulate EMT (2009)

Mutational K-Ras activation arises very frequently in epithelial cancers. However, the specific role of oncogenic K-Ras in maintaining tumor cell survival is unclear. To analyze K-Ras dependency in human cancers we have employed K-Ras-specific shRNAs to deplete K-Ras protein in cancer cell lines derived from lung and pancreatic adenocarcinomas harboring oncogenic K-Ras mutations. By this approach two classes of cell lines were identified - those that are addicted to or dependent on K-Ras activity for viability and those that are independent. We have identified a number of cellular and molecular features that can distinguish these two classes. Firstly, K-Ras dependent cells are epithelial whereas independent cell lines are mesenchymal, as assessed by E-Cadherin and vimentin expression. Secondly, K-Ras dependent cells exhibit high-level focal amplification of the K-Ras gene resulting in increased K-Ras gene copy number and overexpression of mutant K-Ras protein. This phenomenon is reminiscent of addiction to EGFR in lung cancers. Lastly, by comparing gene expression profiles for \#8220;K-Ras-dependent\#8221; and \#8220;K-Ras independent\#8221; cancer cells, we established a gene expression signature that accurately predicts K-Ras dependency and is associated with the differentiation state or histological grade of human lung tumors. We have identified three genes differentially expressed in K-Ras dependent cells that could represent pharmacologically tractable therapeutic targets. These are ITGB6, encoding the integrin beta-6 subunit, as well as SYK and MST1R, which encode tyrosine kinases. Disruption of these genes by RNAi induces epithelial-mesenchymal transformation and apoptosis specifically in K-Ras-dependent cells. Therefore, oncogenic \#8220;K-Ras addiction\#8221; and epithelial differentiation are associated and we suggest that genes regulating epithelial plasticity in K-Ras-addicted cancers may represent novel therapeutic targets. Citation Information: In: Proc Am Assoc Cancer Res; 2009 Apr 18-22; Denver, CO. Philadelphia (PA): AACR; 2009. Abstract nr 1882.