gebhart/patrepeval huggingface dataset:
Supplemental Figure 1 from Notch1 Mutations Are Drivers of Oral Tumorigenesis (2023)

&lt;p&gt;Supplemental Figure 1. List of all NOTCH1 mutations found in Chinese OSCC patients. A. Mutations identified in 22 tumor-normal pairs. B. Mutations found in 28 OSCC samples without matching normal control.&lt;/p&gt;
Proteomic and Metabolomic Characterization of Human Neurovascular Unit Cells in Response to Methamphetamine (2020)

The functional state of the neurovascular unit (NVU), composed of the blood-brain barrier and the perivasculature that forms a dynamic interface between the blood and the central nervous system (CNS), plays a central role in the control of brain homeostasis and is strongly affected by CNS drugs. Human primary brain microvascular endothelium, astrocyte, pericyte, and neural cell cultures are often used to study NVU barrier functions as well as drug transport and efficacy; however, the proteomic and metabolomic responses of these different cell types are not well characterized. Culturing each cell type separately, using deep coverage proteomic analysis and characterization of the secreted metabolome, as well as measurements of mitochondrial activity, the responses of these cells under baseline conditions and when exposed to the NVU-impairing stimulant methamphetamine (Meth) are analyzed. These studies define the previously unknown metabolic and proteomic profiles of human brain pericytes and lead to improved characterization of the phenotype of each of the NVU cell types as well as cell-specific metabolic and proteomic responses to Meth.
Changes in Voiding Patterns in Patients With MS and Extended-Release Oxybutynin (2002)

We evaluated the effects and tolerability of extended-release oxybutynin chloride on the voiding and catheterization frequency of multiple sclerosis (MS) patients with neurogenic bladder. This was a 12-week, prospective, dose-titration study of extended-release oxybutynin (oxybutynin XL). MS patients were recruited for this study from the University of Pittsburgh School of Medicine's MS clinic. Entry criteria included a postvoid residual urine volume of less than 200 mL (in noncatheterized subjects). Previous urodynamic testing was not required. Exclusion criteria included individuals with urine results indicating pyuria in the presence of a positive urine culture. These tests were repeated at six and 12 weeks. After a seven-day washout period, patients recorded episodes of voiding or catheterization and incontinence for three consecutive days. Patients received initial daily doses of 10 mg oxybutynin XL in the first week. Doses were increased at weekly or biweekly intervals to a maximum of 30 mg/d. Tolerability information was collected at each follow-up visit. Twenty of the original 23 patients completed the study. The mean age was 46.3 (range, 24 to 61). Fifteen subjects (75%) were women. Subjects reported clinical improvement with decreased urinary frequency and incontinence episodes after dosing was escalated to 30 mg/d. Seventeen patients chose a final effective dosage greater than 10 mg/d, with 13 patients taking at least 20 mg/d at the end of the study. There were no serious adverse events during the course of the study. Extended-release oxybutynin is safe and effective in MS patients with neurogenic bladder. The onset of clinical efficacy occurs within one week, and daily doses up to 30 mg may be indicated and are well tolerated. (Int J MS Care. 2002; 4: 116–119, 124)
Pretreatment EGFR T790M Mutation and BRCA1 mRNA Expression in Erlotinib-Treated Advanced Non–Small-Cell Lung Cancer Patients with EGFR Mutations (2011)

Low BRCA1 levels neutralized the negative effect of the T790M mutation and were associated with longer progression-free survival to erlotinib. We advocate baseline assessment of the T790M mutation and BRCA1 expression to predict outcome and provide alternative individualized treatment to patients based on T790M mutations and BRCA1 expression.
Detachable Object Detection: Segmentation and Depth Ordering From Short-Baseline Video (2011)

We describe an approach for segmenting an image into regions that correspond to surfaces in the scene that are partially surrounded by the medium. It integrates both appearance and motion statistics into a cost functional, that is seeded with occluded regions and minimized efficiently by solving a linear programming problem. Where a short observation time is insufficient to determine whether the object is detachable, the results of the minimization can be used to seed a more costly optimization based on a longer sequence of video data. The result is an entirely unsupervised scheme to detect and segment an arbitrary and unknown number of objects. We test our scheme to highlight the potential, as well as limitations, of our approach.
Human ACAT-1 and ACAT-2 Inhibitory Activities of Pentacyclic Triterpenes from the Leaves of Lycopus lucidus TURCZ. (2006)

Acyl-CoA: cholesterol acyltransferase (ACAT) catalyzes the acylation of cholesterol to cholesteryl ester with long chain fatty acids and ACAT inhibition is a useful strategy for treating hypercholesterolemia or atherosclerosis. Pentacyclic triterpenes, ursolic acid (1), oleanolic acid (2), and betulinic acid (3) were isolated from the methanol extracts of the leaves of Lycopus lucidus TURCZ. by bioassay-guided fractionation. The structures of compounds 1-3 were elucidated by their spectroscopic data analysis. Among them, betulinic acid (3) exhibited more potent human ACAT-1 and ACAT-2 inhibitory activities with IC(50) values of 16.2+/-0.6 and 28.8+/-1.3 microM, respectively.
Dicyanobis(triphenylphosphine)nickel(II) (2001)

[21946-42-9] C38H30N2NiP2 (MW 635.31) InChI = 1S/2C18H15P.2CN.Ni/c2*1-4-10-16(11-5-1)19(17-12-6-2-7-13-17)18-14-8-3-9-15-18;2*1-2;/h2*1-15H;;; InChIKey = KBSXFMJOIORMAN-UHFFFAOYSA-N (nickel(II) complex useful for the oligomerization of norbornadiene2 and the isomerization of alkenes3) Physical Data: mp 212 °C (dec). Solubility: only sparingly sol most organic solvents; most sol CH2Cl2. Preparative Method: dry nickel(II) cyanide (11 g) was refluxed in 1 L ethanol with 78 g Triphenylphosphine for 48 h. The yellowish-gray Ni(CN)2 turned to a light yellow coarse crystalline mass. The solvent was decanted and the solid was rinsed with ethanol to remove unreacted PPh3. The remaining solid product was dried, extracted with CH2Cl2, and filtered to exclude insoluble Ni(CN)2. Removal of solvent provided 39 g (62%) of light yellow crystals.4 Handling, Storage, and Precautions: air stable. Store at rt.
Localization of cholinergic and purinergic receptors on outer hair cells isolated from the guinea-pig cochlea (1992)

Acetylcholine (ACh) and adenosine 5'-triphosphate (ATP) are shown to act in opposing fashion on guinea-pig cochlear outer hair cells (OHCS) via receptors localized within different fluid compartments of the organ of Corti. The cholinergic (efferent) receptors localized at the basal (perilymphatic) region of these cells activated a rapidly desensitizing hyperpolarizing K+ current. In contrast, purinergic (ATP) receptors were localized at the apical (endolymphatic) surface of OHCS and activated a depolarizing nonselective cation current which exhibited inward rectification and lacked desensitization. Localization of the receptors was determined by using whole-cell patch-clamp, by recording onset latencies and response amplitudes to pulses of either ACh or ATP pressure-applied at selected sites along the length of isolated OHCS. Under voltage-clamp at -60 mV, the largest ACh-induced (outward) currents were recorded when ACh was directed at the basal region of the cells. Conversely, the maximum (inward) ATP currents were obtained when ATP was directed toward the apical surface of these cells. Onset latencies increased rapidly from a minimum of approximately 10 ms for either ACh or ATP as the drug pipette was moved away from these optimal sites. The ATP response was antagonized by amiloride in a dose-dependent manner with a KD of approximately 400 microM. The localization of P2-type purinoceptors to the endolymphatic surface of OHCS suggests that ATP mediates a humoral modulation of the mechano-electrical transduction process.
The evolution of financial toxicity in patients with lung cancer. (2020)

e19406 Background: Financial toxicity (FT), or the cost-related side effect of cancer therapy, has been linked to poor clinical outcomes, greater symptom burden, and worse quality of life. While the repercussions of FT have been thoroughly explored, the longitudinal patient relationship with FT in the initial course of therapy is unknown. Methods: Patients with stage II – IV lung cancer were recruited in a prospective longitudinal non-interventional study between July 2018 and January 2020. FT was measured via the COST score, a validated questionnaire for benchmarking FT, at a) the time of cancer diagnosis and then later at b) 6-month follow up (6MFU). In this model, a lower COST score indicates increased financial hardship. Completed 6MFU data was compared to corresponding baseline data for each patient. Linear regression analysis was used to compare patient characteristics with baseline COST (COST base ) and 6-month COST (COST 6M ) scores. Results: At the time of analysis, 209 patients were screened and 194 (95.1%) patients agreed to participate and complete the baseline survey. Subsequently, 93 patients completed the 6MFU survey, 32 patients had died, and 7 patients were lost to follow up. The remaining 62 patients have not met the study end point to date. Of the 93 patients that completed the 6MFU and baseline survey, the median age was 65 (range 35-89), 50.5% were male, and 76.3% were Caucasian. Over the first six months of therapy 36.5% overestimated OOP expenses by &gt; $1000 and 22.5% reported a decrease in total monetary savings, but only 4.3% of patients made decisions about health care based on cost of care. FT was present at both time points but was worse at diagnosis than at 6MFU (median COST base 25 [range 1-44] vs. median COST 6M 27 [range 0-44]; p = 0.04). Linear regression correlated risk factors with FT at baseline ( &lt; 1 month monetary savings, employed but on sick leave, and inability to afford basic necessities; all p &lt; 0.001) that were different from risk factors at 6MFU (paying much more than expected OOP and sacrificing spending to meet medical costs; p &lt; 0.001). There was no significant difference between estimated OOP costs at baseline (median $2550 [range $0 - $500,000]) and actual OOP costs (median $2496 [range $0 – 25,900]; p = 0.25). Conclusions: FT toxicity is pervasive at both diagnosis and at 6-month follow up, however, the magnitude of toxicity changes with time. Few patients are willing to sacrifice medical care regardless of the cost. Risk factors for FT evolve, suggesting that different groups may benefit from financial intervention at diagnosis versus 6MFU.
A Low-Power WCDMA Transmitter With an Integrated Notch Filter (2008)

A filtering technique to attenuate the receive-band noise enables a 65-nm CMOS WCDMA transmitter to achieve an output noise level of -160 dBc/Hz at 80-MHz offset, while dissipating 65 mW. Using a feedback filtering technique, the circuit introduces a null with an arbitrary width at the receive frequency and eliminates the need for an external SAW filter. A stability analysis is performed to choose the most suitable architecture for the feedback path, which ensures stability over a wide range of loop-gain. Nonideal effects such as quadrature phase and gain errors, LO feedthrough, harmonic downconversion and phase-noise are also analyzed.
Computational costs versus benefits of control reasoning (1987)

These items related to reading peer-reviewed professional journals, critically reading professional literature, relevance and importance of evidence-based clinical practice, and level of comfort with knowledge in research.
Long‐term safety of oral nucleos(t)ide analogs for patients with chronic hepatitis B: A cohort study of 53,500 subjects (2015)

NA treatment does not increase the risk of renal and bone events in general. Nucleotide analogs may increase the risk of hip fracture, but the overall event rate is low.
Abstract 3024: Sensitivity of Molecular MRI for Low Levels of Cardiomyocyte Apoptosis in an In-Vivo Model of Dilated Cardiomyopathy (2006)

Background: Cardiomyocyte (CM) apoptosis has been successfully imaged in-vivo by MRI in acutely injured myocardium. However, significantly lower levels of CM apoptosis are seen in dilated cardiomyo...
REPEATED ARTHROSCOPY OF THE SHOULDER: RESULTS AND INDICATIONS IN A SERIES OF 24 CASES (2002)

Purpose: Arthroscopic arthroplasty for painful shoulder is not always successful. Repeated arthroscopy is sometimes discussed for patients with recurrent pain. The purpose of this work was to determine the technique and identify indications. Material and methods: We conducted a retrospective analysis of 24 patients (13 men, 11 women), mean age 52 years, who underwent repeated arthroscopy after failure within 31 months of arthroscopic acromioplasty with no other intervention. Three groups were formed: group 1 (7 patients): subacromial impingement due to tendinitis with intact supraspinatus; group 2 (11 patients): subacromial impingement secondary to cuff tear; group 3 (6 patients): calcified tendinopathy. In group 1, the acromion was type 1 in three cases, type 2 in three and type 3 in one; repeated arthroscopy included complementary acromioplasty. In group 2, all patients had complementary acromioplasty; six of them with biceps tenotomy. In group 3, the remaining calcification was removed in all patients and complementary acromioplasty in three. Results: Mean follow-up was 21 months. In group 1, outcome was satisfactory in three patients (43%), irrespective of the acromial morphology. In group 2, outcome was satisfactory in six patients (55%), including five with acromioplasty with tenotomy and only one with complementary acromioplasty alone. In group 3, outcome was satisfactory in five patients (83%); one failure was attributed to remaining calcification; acromioplasty did not influence outcome. Discussion, conclusion: The acromion should not be considered as the principal cause of failure after first acromioplasty. In this series, only 33% of the patients who had complementary acromioplasty had a good outcome at last follow-up. Biceps tenotomy in patients with rotator cuff tears and removal of the calcium deposit in patients with calcified tenopathies should be considered first.
Supplementary Data from Comprehensive Serum Proteome Profiling of Cytokine Release Syndrome and Immune Effector Cell–Associated Neurotoxicity Syndrome Patients with B-Cell ALL Receiving CAR T19 (2023)

Supplementary Data from Comprehensive Serum Proteome Profiling of Cytokine Release Syndrome and Immune Effector Cell–Associated Neurotoxicity Syndrome Patients with B-Cell ALL Receiving CAR T19
Overexpression of an alternative allele of carboxyl/choline esterase 4 (CCE04) of Tetranychus urticae is associated with high levels of resistance to the keto‐enol acaricide spirodiclofen (2019)

Abstract BACKGROUND Spirodiclofen is an acaricide that targets lipid biosynthesis by inhibiting acetyl‐coenzyme A carboxylase. Spirodiclofen resistance in spider mites has been previously documented and was associated with overexpression of CYP392E10, a cytochrome P450 mono‐oxygenase that metabolizes spirodiclofen. However, additional mechanisms have been suggested in several studies and a carboxyl/choline esterase gene, CCE04 , was shown to be overexpressed in two genetically different strains, SR‐VP and SR‐TK, both exhibiting high spirodiclofen resistance levels. RESULTS We identified two different CCE04 alleles in both resistant strains, CCE04 SR‐VP and CCE04 London , with CCE04 SR‐VP being highly overexpressed. Isoelectric focusing analysis confirmed the overexpression of a single esterase isozyme, while copy number and random fragment length polymorphism analysis revealed that CCE04 SR‐VP overexpression was more likely due to selection for the CCE04 SR‐VP allele rather than gene amplification. Both CCE04 alleles were functionally expressed using the Pichia expression system. Functional enzyme assays revealed only limited kinetic differences between CCE04 isoforms for model substrates. In addition, inhibition/competition experiments with spirodiclofen suggested a similar interaction with both enzymes, whereas its active metabolite, spirodiclofen enol, did not inhibit enzyme activity. CONCLUSION Our study suggests that selection with spirodiclofen results in enrichment of a specific allele of CCE04 ( CCE04 SR‐VP ) in two genetically independent strains, which is highly overexpressed. Based on kinetic enzyme data, however, quantitative rather than qualitative differences between CCE04 SR‐VP and CCE04 London seem more likely to be involved in resistance. Our findings are discussed in the light of a possible spirodiclofen resistance mechanism, with sequestration of spirodiclofen by CCE04 SR‐VP being a likely hypothesis. © 2019 Society of Chemical Industry
PS-B05-10: DIABETES MELLITUS AGGRAVATES BEHAVIORAL DEFICIT AND INCREASES SITE-SPECIFIC PHOSPHORYLATION OF TAU PROTEIN IN ALZHEIMER'S DISEASE MOUSE MODEL (2023)

Objective: Numerous epidemiological studies have demonstrated that vascular risk factors including diabetes mellitus (DM) increase the risk of developing Alzheimer's disease (AD). However, the mechanisms underlying the pathological relationship between DM and AD remain largely unknown. Tau is a microtubule-associated protein that is predominantly expressed in the axons of neurons. More than 80 residues of tau can be potentially phosphorylated. Accumulation of hyperphosphorylated tau protein plays a role in neurodegeneration and cognitive impairment in patients with AD. Here, we developed a unique diabetic AD mouse model and analyzed behavioral phenotypes and biochemical changes occurring in the brain to investigate the impact of DM on tau pathology. Design and method: Tau-tg and WT mice were fed with either normal chow diet (NCD; 12% kcal fat, n = 9) or high-fat diet (HFD; 60% kcal fat, n = 10) from the age of one and a half to nine months. Metabolic parameters and behavioral phenotypes were assessed at eight months of age. Mice were then sacrificed at nine months of age and brains were analyzed histologically and biochemically. A quantitative proteomic analysis of protein phosphorylation was performed using brain extracts of tau-tg mice. Results: Tau-tg mice fed with HFD (HFD tau-tg) showed severe obesity and hyperinsulinemia compared with tau-tg mice fed with NCD (NCD tau-tg) (p &lt; 0.01), with slight elevation of plasma glucose levels. HFD tau-tg showed exacerbation of motor impairments and decrement of habituation to novel environment. Histological analysis showed a significant decrease in the number of neurons in HFD tau-tg compared to NCD tau-tg. The amount of aggregated form of phospho-tau (Ser396) was increased in HFD tau-tg brain. Comprehensive phosphoproteomic analysis identified 11 phosphosites of tau significantly hyperphosphorylated in the brain of HFD tau-tg (p &lt; 0.05). Conclusions: These results suggest that diabetic conditions such as obesity and hyperinsulinemia with minimal hyperglycemia exacerbate behavioral deficits in AD via hyperphosphorylation of tau protein. We identified specific phosphosites of tau involved in pathological relationship between DM and AD.
Capillary gas chromatography with ultraviolet spectrometric detection (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCapillary gas chromatography with ultraviolet spectrometric detectionM. Novotny, F. J. Schwende, M. J. Hartigan, and J. E. PurcellCite this: Anal. Chem. 1980, 52, 4, 736–740Publication Date (Print):April 1, 1980Publication History Published online1 May 2002Published inissue 1 April 1980https://pubs.acs.org/doi/10.1021/ac50054a032https://doi.org/10.1021/ac50054a032research-articleACS PublicationsRequest reuse permissionsArticle Views84Altmetric-Citations23LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Comparison of Subtypes of Listeria monocytogenes Isolates from Naturally Contaminated Watershed Samples with and without a Selective Secondary Enrichment (2014)

Two enrichment methods for Listeria monocytogenes using Immuno Magnetic Separation (IMS) were tested to determine if they selected the same subtypes of isolates. Both methods used a non-selective primary enrichment and one included subculture in Fraser Broth, while the other involved direct plating of IMS beads. Sixty-two naturally contaminated watershed samples from the Central California Coast were used as a source of L. monocytogenes, and subtype diversity was measured by serotype and Multiple Number Variable Tandem Repeat Analysis (MLVA). Three different serotypes were detected from both methods with serotype 4b strains making up 87% of the isolates, serotype 1/2a making up 8%, and serotype 1/2b making up 5%. The data suggest that serotype 1/2a strains were more likely to be isolated from the Fraser Broth culture method. Sixty-two different MLVA types were detected and the more common MLVA types were detected by both culture methods. Forty-three MLVA types were detected only from one culture method or the other, while 19 types were detected from both culture methods. The most common MLVA type-12 was detected in 33 of the 62 water samples, and represented 31% of the isolates from both culture methods. This limited study provides evidence that using both enrichment culture methods allowed for detection of a greater diversity of isolates among the samples than the use of one method alone, and that a wide diversity of L. monocytogenes strains exist in this watershed.