gebhart/patrepeval huggingface dataset:
Biodynamic Response of the Musculoskeletal System to Impact Acceleration (1980)

&lt;div class="htmlview paragraph"&gt;The effect of muscular response on occupant dynamics was studied in human volunteers exposed to low level impact acceleration. The study includes identification of muscular response, correlation of electromyographic activity with reaction force, and investigation of the effects of muscular restraint during impact. Human volunteers were subjected to −G&lt;sub&gt;x&lt;/sub&gt; impact acceleration in a simulated automobile environment while EMG activity of various lower extremity muscles was monitored. The seat and floor pan were supported on load cells which measured all restraining forces. Nine–accelerometer modules and high-speed photography were used to measure kinematics. Identical runs were made with an embalmed cadaver and dummy for comparison.&lt;/div&gt; &lt;div class="htmlview paragraph"&gt;Static EMG and force traces as well as dynamic results for various acceleration levels are presented. Differences between tensed and relaxed states are compared and discussed as to EMG response, force levels, and head kinematics.&lt;/div&gt; &lt;div class="htmlview paragraph"&gt;It was found that reflex responses of the relaxed volunteer are too slow to have a significant effect on loads and accelerations sustained. However, the voluntary pre-impact contracted musculature in a volunteer can reduce certain acceleration levels and change the restraint load distribution so that significantly more load goes through the legs to the floor board with a concomitant lowering of seat and belt loads. Although a similar load distribution was seen in dummy and cadaver tests, the response of the relaxed or tensed volunteer was substantially different from either surrogate.&lt;/div&gt;
Supplementary Figure 2 from Metalloprotease-Mediated Tumor Cell Shedding of B7-H6, the Ligand of the Natural Killer Cell–Activating Receptor NKp30 (2023)

&lt;p&gt;PDF file - 79KB, Pharmacological inhibition of protein disulfide isomerases reduces soluble B7-H6.&lt;/p&gt;
Benchmarking the dynamic luminescence properties and UV stability of B 18 H 22 -based materials (2022)

The dynamic photoluminescence properties, and potential quenching mechanisms, of <i>anti</i>-B<sub>18</sub>H<sub>22</sub>, 4,4'-Br<sub>2</sub>-<i>anti</i>-B<sub>18</sub>H<sub>20</sub>, and 4,4'-I<sub>2</sub>-<i>anti</i>-B<sub>18</sub>H<sub>20</sub> are investigated in solution and polymer films. UV stability studies of the neat powders show no decomposition occurring after intense 7 day light soaking. In contrast, clusters incorporated into polymer films are found to degrade into smaller borane fragments under the same irradiation conditions. To highlight the utility of these compounds, we leverage their favorable optical properties in a prototype UV imaging setup.
Synthesis of Lipidated Green Fluorescent Protein and Its Incorporation in Supported Lipid Bilayers (2005)

Herein we report a semisynthetic method of producing membrane-anchored proteins. Ligation of synthetic lipids with designed anchor structures to proteins was performed using native chemical ligation (NCL) of a C-terminal peptide thioester and an N-terminal cysteine lipid. This strategy mimics the natural glycosylphosphatidylinositol (GPI) linkage found in many natural membrane-associated proteins; however, the synthetic method utilizes simple lipid anchors without glycans. Synthetically lipidated recombinant green fluorescent protein (GFP) was shown to be stably anchored to the membrane, and its lateral fluidity was quantitatively characterized by direct fluorescence imaging in supported membranes. Circumventing the steps of purification from native cell membranes, this methodology facilitates the reconstitution of membrane-associated proteins.
Guest Editorial Special Issue on Computational Intelligence in Telecommunications Networks and Internet Services—Part III (2004)

A variety of detailed technologies, concepts, and models aim at the realization of intelligent integrated networks and services. Active and programmable networking enable the introduction of new network services by adding dynamic programmability to network devices and making aspects of the programmability accessible to third-party vendors and users via open interfaces. Open and programmable network elements help redefine network software architectures and move control and management systems away from traditional closed and rigid solutions, bringing us one step closer to an environment where all devices and services seamlessly interoperate and cooperate with each other. Computational intelligence (CI) has emerged through a vivid and diversified synergy of technologies of granular computing, neural networks, and evolutionary computing. Each of the contributing technologies there plays an important and unique role. In essence, we can state that CI is a manifestation and implementation of a general design methodology applied to complex systems. The article reviews this special issue.
Ten-year in vivo wear measurement of a fully congruent mobile bearing unicompartmental knee arthroplasty (2005)

Polyethylene particulate wear debris continues to be implicated in the aetiology of aseptic loosening following knee arthroplasty. The Oxford unicompartmental knee arthroplasty employs a spherical femoral component and a fully congruous meniscal bearing to increase contact area and theoretically reduce the potential for polyethylene wear. This study measures the in vivo ten-year linear wear of the device, using a roentgenstereophotogrammetric technique. In this in vivo study, seven medial Oxford unicompartmental prostheses, which had been implanted ten years previously were studied. Stereo pairs of radiographs were acquired for each patient and the films were analysed using a roentgen stereophotogrammetric analysis calibration and a computer-aided design model silhouette-fitting technique. Penetration of the femoral component into the original volume of the bearing was our estimate of linear wear. In addition, eight control patients were examined less than three weeks post-insertion of an Oxford prosthesis, where no wear would be expected. The control group showed no measured wear and suggested a system accuracy of 0.1 mm. At ten years, the mean linear wear rate was 0.02 mm/year. The results from this in vivo study confirm that the device has low ten-year linear wear in clinical practice. This may offer the device a survival advantage in the long term.
EPCT-03. SERIAL PLASMA AND CSF CELL-FREE TUMOR DNA (CF-TDNA) TRACKING IN DIFFUSE MIDLINE GLIOMA PATIENTS UNDERGOING TREATMENT WITH ONC201 (2021)

Abstract Diffuse midline glioma (DMG) with the H3K27M mutation is a lethal childhood brain cancer, with patients rarely surviving 2 years from diagnosis. We conducted a multi-site Phase 1 trial of the imipridone ONC201 for children with H3K27M-mutant glioma (NCT03416530). Patients enrolled on Arm D of the trial (n=24) underwent serial lumbar puncture (baseline, 2, 6 months) for cell-free tumor DNA (cf-tDNA) analysis at time of MRI. Additionally, patients on all arms of the trial at the University of Michigan underwent serial plasma collection. CSF collection was feasible in this cohort, with no procedural complications. We collected 96 plasma samples and 53 CSF samples from 29 patients, including those with H3F3A (H3.3) (n=13), HIST13HB (H3.1) (n= 4), and unknown H3 status/not biopsied (n=12) [range of 0–8 CSF samples and 0–10 plasma samples]. We performed digital droplet polymerase chain reaction (ddPCR) analysis and/or amplicon-based electronic sequencing (Oxford Nanopore) of cf-tDNA samples and compared variant allele fraction (VAF) to radiographic change (maximal 2D tumor area on MRI). Preliminary analysis of samples demonstrates a correlation between changes in tumor size and H3K27M cf-tDNA VAF, when removing samples with concurrent bevacizumab. In multiple cases, early reduction in CSF cf-tDNA predicts long-term clinical response (&amp;gt;1 year) to ONC201, and does not increase in cases of later-defined pseudo-progression (radiation necrosis). For example, a now 9-year old patient with thalamic H3K27M-mutant DMG underwent treatment with ONC201 after initial radiation and developed increase in tumor size at 4 months post-radiation (124% baseline) of unclear etiology at the time. Meanwhile, her ddPCR declined from baseline 6.76% VAF to &amp;lt;1%, which has persisted, with now near complete response (15% tumor reduction) at 30 months on treatment from diagnosis. In summary, we present the feasibility and utility of serial CSF/plasma monitoring of a promising experimental therapy for DMG.
Constructing travel itineraries from tagged geo-temporal breadcrumbs (2010)

Vacation planning is a frequent laborious task which requires skilled interaction with a multitude of resources. This paper develops an end-to-end approach for constructing intra-city travel itineraries automatically by tapping a latent source reflecting geo-temporal breadcrumbs left by millions of tourists. In particular, the popular rich media sharing site, Flickr, allows photos to be stamped by the date and time of when they were taken, and be mapped to Points Of Interest (POIs) by latitude-longitude information as well as semantic metadata (e.g., tags) that describe them.
Fragmentation des Closo‐N‐Methyl Phosphorimide, Thiophosphorimides et Arsenimide (1974)

Abstract Les spectres de masse du closo phosphorimide P 4 (NCH 3 ) 6 S n , où n = 1 à 4, et du closo arsenimide As 4 (NCH 3 ) 6 ont été et des schémas de fragmentations systématiques ont été dégagés, L'introduction des groupes thiophosphoryles se traduit par une superposition des deux types de fragmentations identifiées pour P 4 (NCH 3 ) 6 P 4− dérivés ou de l'hexaméthylène tétramine.
Rate-Limited Secure Function Evaluation: Definitions and Constructions. (2013)

Abstract. We introduce the notion of rate-limited secure function evaluation (RL-SFE). Loosely speaking, in an RL-SFE protocol participants can monitor and limit the number of distinct inputs (i.e., rate) used by their counterparts in multiple executions of an SFE, in a private and verifiable manner. The need for RL-SFE naturally arises in a variety of scenarios: e.g., it enables service providers to “meter ” their customers ’ usage without compromising their privacy, or can be used to prevent oracle attacks against SFE constructions. We consider three variants of RL-SFE providing different levels of security. As a stepping stone, we also formalize the notion of commit-first SFE (cf-SFE) wherein parties are committed to their inputs before each SFE execution. We provide compilers for transforming any cf-SFE protocol into each of the three RL-SFE variants. Our compilers are accompanied with simulation-based proofs of security in the standard model and show a clear tradeoff between the level of security offered and the overhead required. Moreover, motivated by the fact that in many client-server applications clients do not keep state, we also describe a general approach for transforming the resulting RL-SFE protocols into stateless ones. As a case study, we take a closer look at the oblivious polynomial evaluation (OPE) protocol of
Abstract 159: Analysis of resistance to the combination of a PI3K- and Parp-inhibitor using a genomic sequencing approach (2016)

Abstract The objective of this study is to investigate the genomic and transcriptonal changes that are associated with resistance to treatment with the combination of a PI3K-inhibitor (NVP-BKM120) and a PARP-inhibitor (Olaparib). Previous studies have shown that the combination of a PI3K-inhibitor and a PARP-inhibitor synergistically reduced the growth of BRCA-related xenograft tumors derived from patients with TNBC. However, in clinical practice, primary and secondary resistance to this combination is observed. We hypothesize that acquired resistance is the result of a genomic evolution resulting from the selection pressure exerted by the drug treatment. We used a genetically engineered mouse model, K14-Cre BRCA1f/fp53f/f, where primary tumors had been propagated in Cre-negative littermates and treated to point of resistance with NVP-BKM120 or NVP-BKM120 plus Olaparib. For each of the three tumors, we analyzed exome sequencing and RNA-seq using Illumina technology at the stage of sensitivity (C), resistance to PI3K-inhibition (B) and resistance to the combination (BO); i.e. for each individual tumor a parental clone and its drug-resistant subclones, obtained in vivo, were analyzed. We used the Mutect software tool to call somatic mutations, VarScan2 to call somatic copy number variations, Cuffdiff for differential expression analysis, and TophatFusion. Data were integrated to identify genes for functional analyses using MetaCore and MSigDB software tools. We found that tumors resistant to the combination drug treatment in general had fewer gross genomic alterations (CNVs) than parental tumors, indicative of evolution of a less variable subclone. However, these tumors also displayed a higher number of non-synonymous mutations than their parental clone. Pathway analysis showed that somatically mutated genes in PI3K-inhibitor resistant tumors were highly enriched for antigen processing and presentation, while those of tumors resistant to the combination treatment were enriched for histone modification pathways. Notably, the tumors resistant to PI3K-inhibitor alone were enriched for Insulin-processing pathways. Genomic losses in PI3K-inhibitor treated tumors enriched for the estrogen receptor pathway (ESR) in breast cancer and DNA damage pathways. In conclusion, our experimental design of analyzing isogenic tumors resistant to PI3K- or combined PI3K- and Parp-inhibitors enabled us to identify genomic alterations and pathways that explain the evolution to drug resistance. Citation Format: Sheida Nabavi, Ashish Juvekar, Nicholas Wang, Lewis C. Cantley, Gerburg M. Wulf. Analysis of resistance to the combination of a PI3K- and Parp-inhibitor using a genomic sequencing approach. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 159.
The Detection and Characterization of Ions,DNA, and Proteins Using Nanometer‐Scale Pores (2007)

Abstract Protein ion channels are nanometer‐scale pores that are central to many biological processes, including the sensing of a wide variety of molecules. They have also demonstrated potential for use in the sensitive and selective detection of ions, the characterization of polynucleotides and toxins as well as the screening of therapeutic agents against lethal anthrax proteins. Further advances in materials engineering, theory, and signal processing are needed to enable practical applications based on biological or synthetic nanopores.
High Performance AEM Water Electrolysis with PGM-Free Electrocatalysts (2022)

Water electrolysis technologies for hydrogen production are getting much attention due to drastic cost reduction in renewable energy sources, like solar, wind, tidal etc. Traditional alkaline water electrolysis has limitations of low current density operation, slow system response and low hydrogen discharge pressure. Proton exchange membrane (PEM) water electrolysis offers compact design, high current density operation, fast system response and pressurized discharge hydrogen. However, PEM electrolyzers require the use of Platinum Group Metal (PGM) based electrocatalysts, expensive perfluorinated membranes and specialized component materials due to its acidic environment. These are all hurdles to its widespread commercial adoption. A relatively new technology, the anion exchange membrane (AEM) electrolyzer can potentially combine benefits from PEM and traditional alkaline electrolyzers, offering high current density operation, pressurized discharge gas and low cost – by utilizing PGM-free electrocatalysts and inexpensive component materials due to the less corrosive alkaline operating environment. However, modern AEM electrolyzers have continued to use high loadings of PGM catalysts in both the cathode and anode. Given the magnitude and recent volatility in the market price of many PGM-group metal catalysts (e.g. Ru, Ir, etc.), it is now even more important for AEM electrolyzers to be realized with significantly lower PGM content – and eventually approaching the complete elimination of PGMs. In this study, we evaluate the performance of several low-PGM and PGM-free electrocatalysts for the oxygen evolution (OER) and hydrogen evolution (HER) reactions for high performance and durability. Here, PGM-free Lanthanum Strontium Cobalt (LSC), Nickel Ferrite (NiFeOx) and low PGM Lead Ruthenate (PbRuOx) were used at the anode for the OER. For the HER cathode, PGM-free Nickel Molybdenum (NiMo) and low-PGM PtNi electrocatalysts were evaluated for their in-situ activity and durability. It will be shown that LSC and NiFeOx show comparable performance to IrOx, with a typical steady-state operating voltage at 60 o C and 1.0 A/cm 2 (with 0.3 M KOH fed to the anode only) below 1.80 V. Cells with PGM-free anode catalysts were operated stably for over 100 hours. At the cathode, NiMo showed relatively higher overpotentials compared to Pt black, PtNi or Pt/C for the HER. Because of this, various strategies were adopted to reduce the PGM loading while achieving high performance and durable AEM electrolyzer operation. The achieved experimental results provide important insights for the development of AEM based water electrolyzer systems and represent an active step towards its commercial viability.
Patient-specific modelling of blood flow dynamics in intra-cranial aneurysms: an example of image-based personalised medicine (2009)

The visualisation of blood flow dynamics in intracranial aneurysm using computational fluid dynamics is an example of personalised medicine based on biomedical image analysis. A small percentage of these aneurysms rupture. Blood flow dynamics is thought to play an important role in the rupture process by exerting Wall Shear Stresses (WSS) on the endothelial cells. In this study, the effects of instabilities in the velocity waveform in the parent artery of an aneurysm of the Internal Carotid Artery (ICA) and variations of the computational mesh are investigated with respect to WSS at the aneurysm wall.
Exposure to chemicals, physical agents, and biologic agents in mycosis fungoides and the Sézary syndrome. (1979)

The history of occupational, environmental, and/or iatrogenic exposure to potential carcinogenic agents was obtained at the time of onset of skin disease in 43 of 44 patients with cutaneous T-cell lymphoma (mycosis fungoides and the Sézary syndrome) entering a National Cancer Institute therapeutic trial. A history of multiple exposures to these agents was common, the two most frequent being chemicals (91% of patients) and drugs (86%). Mean duration of expsosure was 13 years for chemicals and 18 years for drugs. The most common chemicals were air pollutants (39%), pesticides (36%), solvents and vapors (30%), and detergents and disinfectants (14%). Increased severity of disease was seen with increaed duration of chemical exposure in stage V cutaneous T-cell lymphomas only. The most frequent drugs besides tobacco (86%) were analgesics (20%), tranquilizers (18%), and thiazides (14%). Second cancers occurred in four patients, including two renal cell carcinomas, and a family history of cancer was present in 11. Fourteen of 22 patients questioned had recurrent herpes simplex. Patients with chronic skin disease who have long-term exposure to combinations of chemicals, physical agents, and biologic agents, are heavy smokers, or have recurrent herpes simplex appear to be prime candidates for developing mycosis fungoides or the Sézary syndrome.
The anti‐inflammatory compound BAY‐11‐7082 is a potent inhibitor of protein tyrosine phosphatases (2013)

The families of protein tyrosine phosphatases (PTPs) and protein tyrosine kinases (PTKs) function in a coordinated manner to regulate signal transduction events that are critical for cellular homeostasis. Aberrant tyrosine phosphorylation, resulting from disruption of either PTP or PTK function, has been shown to be the cause of major human diseases, including cancer and diabetes. Consequently, the characterization of small-molecule inhibitors of these kinases and phosphatases may not only provide molecular probes with which to define the significance of particular signaling events, but also may have therapeutic implications. BAY-11-7082 is an anti-inflammatory compound that has been reported to inhibit IκB kinase activity. The compound has an α,β-unsaturated electrophilic center, which confers the property of being a Michael acceptor; this suggests that it may react with nucleophilic cysteine-containing proteins, such as PTPs. In this study, we demonstrated that BAY-11-7082 was a potent, irreversible inhibitor of PTPs. Using mass spectrometry, we have shown that BAY-11-7082 inactivated PTPs by forming a covalent adduct with the active-site cysteine. Administration of the compound caused an increase in protein tyrosine phosphorylation in RAW 264 macrophages, similar to the effects of the generic PTP inhibitor sodium orthovanadate. These data illustrate that BAY-11-7082 is an effective pan-PTP inhibitor with cell permeability, revealing its potential as a new probe for chemical biology approaches to the study of PTP function. Furthermore, the data suggest that inhibition of PTP function may contribute to the many biological effects of BAY-11-7082 that have been reported to date.
Blood Cell Capture on Antibody Microarrays and Monitoring of the Cell Capture Using Surface Plasmon Resonance Imaging (2010)

Blood is a tremendous source of data for diagnostic purposes. Thanks to the qualitative and quantitative analysis of the different cell types carried into the blood stream. To that end, cell capture of several cell types at different locations on a microarray is an interesting alternative to classical techniques run 'in solution' as it allows simultaneous characterization of several cells on a single device. In this chapter, we have described a method based on the production of antibody microarrays specific to two different cell types: B and T lymphocytes. We have also described the real-time monitoring of the cell capture on the microarray using surface plasmon resonance imaging (SPRi).
Miniaturized piezo electric chopper for fiber optical multimode applications realized using LIGA-technology (2002)

A miniaturized chopper for fiber optical multimode applications has been developed at the Institut fur Mikrostrukturtechnik at Forschungszentrum Karlsruhe. Transmittance is modulated by a piezo electric driven shutter, moving in a gap between two multimode fibers.
Multiband subwavelength magnetic reflectors based on fractals (2003)

We use theory and experiment to demonstrate that a composite material, consisting of a metallic planar fractal and a metal sheet separated by a thin dielectric layer, can reflect electromagnetic wave in-phase at a series of frequencies, with some of the corresponding wavelengths much longer than the reflector’s own size. We show that the physics is governed by a series of intrinsic magnetic resonances and can be well described by an effective-media of frequency-dependent permeability.
ORNL Bioenergy technologies (2014)

ORNL researchers discuss breakthroughs in biomass conversion, feedstocks, logistics and sustainability.