gebhart/patrepeval huggingface dataset:
CATECHOLAMINES AND METABOLISM OF HUMAN ADIPOSE TISSUE (1970)

Abstract. The influence of glucose on the in vitro lipolysis of human omental adipose tissue has been studied. Omental tissue obtained at surgery during general anesthesia was incubated in Krebs‐Henseleit bicarbonate buffer containing 3% bovine albumin with and without added glucose (2 mg/ml). Glycerol release into the medium was considered as an index of lipolysis. Glucose enhanced the basal lipolysis and potentiated significantly the adipokinetic effects of noradrenaline, isopropylnoradrenaline and N 6 ‐2′0‐dibutyryl cAMP (dibutyryl cAMP). The slope of glycerol release obtained for dibutyryl cAMP in glucose‐free versus glucose‐containing medium was significantly different from those obtained for noradrenaline and isopropylnoradrenaline. This difference might be explained if it is admitted that glucose stimulates lipolysis by acting on two different sites: ( a ) close to the triglyceride‐splitting enzyme system, and ( b ) close to the beta‐adrenergic receptor. In a second series of experiments the effect of glucose on the level of FFA was studied in tissue and in medium with noradrenaline present. No changes in the net release of FFA were observed, whereas a slight increase in the tissue level of FFA was demonstrated. These findings in human tissue are not in accordance with those previously described in rat epididymal fat pad, and do not justify the assumption that a decrease in the tissue level of FFA is the “trigger mechanism” for the lipolysis‐promoting effect of glucose. A marked decrease in the glycerol release was noticed when 2‐deoxy‐ d ‐glucose was added to medium containing noradrenaline, isopropylnoradrenaline and dibutyryl cAMP. The data are consistent with the view that glucose metabolism and the activity of triglyceride lipase are closely related.
Benefit of Anti-hepatitis B Immunoglobulin(HBIG) Monotherapy Compared to HBIG Combined with Entecavir in Long Term Renal Function (2018)

Background To reduce the HBV reinfection after liver transplantation, anti-hepatitis B immunoglobulin (HBIG) alone or combination with antiviral nucleotide analogues are usually used regimen. However, antiviral nucleotide analogues have nephrotoxicity, which is a critical issue because renal dysfunction frequently happens after liver transplantation. Method Medical records of 171 liver recipients with HBV who underwent liver transplantation between Sep. 2005 and Dec. 2012 were retrospectively reviewed. The difference of renal function of HBIG mono-therapy group (HBIG) and HBIG combined with Entecavir group (HBIG+ETV) were analyzed. Results There was no significant difference in age, gender, body mass index, intraoperative blood loss, and MELD score between the two groups. But the patients who had preoperative ascites, mean preoperative AST level, preoperative GFR level, and the applying event of CRRT was significantly different between the groups. The decrease of eGFR between preoperative and 1 year after transplantation was 31.5±27.2 mL/min/1.73m2 (p<0.001), in HBIG group and 40.0±45.9 mL/min/1.73m2 (p<0.001) in HBIG+ETV group. Also, the eGFR decrease between preoperative and 4-year after transplantation was 24.5±29.9 mL/min/1.73m2 (p<0.001) in HBIG group and 38.9±56.8 mL/min/1.73m2 (p<0.001) in HBIG+ETV group. Conclusion There was no difference of recurrence rate of HBV. However, HBIG+ETV combination regimen showed more declination of eGFR in long-term period after liver transplantation than HBIG alone.
Dynamic Response Analysis of Rotor-Bearing Systems With Cracked Shaft (2002)

A general dynamic model for a large-scale rotor-bearing system with a cracked shaft is introduced. A finite shaft element with a crack is developed using a consistent finite element approach. The model accommodates shafts with tapered portions, multiple disks and anisotropic bearings. The formulation is applicable to rotor-bearing systems with different practical design configurations including intermediate bearings, shaft overhang, and stepped shaft assemblies. A reduced order form of equations of motion is obtained by invoking the actual non-planar (complex) modal transformations. The time-response due to different excitations are calculated, and comparisons are presented to establish the validity and efficiency of the reduced order model. It is hoped that the developed computational scheme offers an efficient and essential core module in establishing other specialized crack detection schemes for rotor-bearing systems.
O1‐12‐04: Retinal microvascular changes in vascular cognitive impairment compared to ischemic stroke (2015)

There is increasing evidence that microvascular pathology is important in both Alzheimer's disease (AD), vascular dementia (VaD) and stroke. Retinal microvascular damage, measured by advanced retinal vascular imaging, has been shown to be associated with cerebrovascular disease and AD. It remains unclear if retinal microvascular changes are different in dementia patients with concurrent cerebrovascular disease, compared to persons with only cerebrovascular disease. We aimed to examine whether retinal microvascular changes are different in dementia patients with cerebrovascular disease compared to acute ischemic stroke patients. Clinically diagnosed dementia cases (age 45-80 years) with significant cerebrovascular disease (SCeVD) on neuroimaging (MRI or CT scans) were recruited from tertiary hospitals in Singapore. Cases were classified as VaD or AD according to established criteria. Acute ischemic stroke patients were selected and matched by ethnicity, sex and 5-year age group to dementia cases. Retinal vascular parameters (caliber, fractal dimensions and tortuosity) were measured using a computer-based program (Singapore I Vessel Assessment, software version 3.0) from digital retinal photographs, following to a standardized grading protocol. Logistic regression models were used to calculate odds ratios and 95% confidence intervals [OR (95%CI)] for dementia subtype, using ischemic stroke as the reference group, adjusting for ethnicity, sex, age, hypertension, diabetes, hypercholesterolemia and smoking. A total of 85 dementia patients (52 AD with SCeVD, 33 VaD) and 85 stroke patients were matched for analysis. Compared to stroke patients, retinal venular narrowing was associated with AD with SCeVD [OR per SD decrease: 8.47 (3.61-19.9)] and VaD [OR per SD decrease: 5.76 (2.38-13.9)]. Increased arteriolar and venular tortuosity were associated with AD with SCeVD [ORs per SD increase: arteriolar 1.74 (1.14-2.67), venular 1.66 (1.04-2.66)], and VaD [OR per SD increase: arteriolar 1.95 (1.28-2.96), venular 1.98 (1.23-3.20)]. Retinal vascular parameters were not significantly different in AD with SCeVD compared to VaD. Patients with SCeVD who have AD or VaD had narrower retinal venules and increased vessel tortuosity compared to patients with acute ischemic stroke, suggesting that retinal microvascular changes may be useful in identifying patients at risk of developing dementia in persons with stroke.
Symmetric-key Corruption Detection : When XOR-MACs Meet Combinatorial Group Testing. (2019)

We study a class of MACs, which we call corruption detectable MAC, that is able to not only check the integrity of the whole message, but also detect a part of the message that is corrupted. It can be seen as an application of the classical Combinatorial Group Testing (CGT) to message authentication. However, previous work on this application has an inherent limitation in its communication cost. We present a novel approach to combine CGT and a class of linear MACs (XOR-MAC) that breaks this limit. Our proposal, \(\textsf {XOR}\text {-}\textsf {GTM} \), has a significantly smaller communication cost than any of the previous corruption detectable MACs, while keeping the same corruption detection capability. Our numerical examples for storage application show a reduction of communication by a factor of around 15 to 70 compared with previous schemes. \(\textsf {XOR}\text {-}\textsf {GTM} \) is parallelizable and is as efficient as standard MACs. We prove that \(\textsf {XOR}\text {-}\textsf {GTM} \) is provably secure under the standard cryptographic assumptions.
Integrated transcriptome and proteome analysis reveals molecular responses of soybean anther under high-temperature stress (2023)

It is well documented that high temperature (HT) severely affects the development of soybean male reproductive organs. However, the molecular mechanism of thermo-tolerance in soybean remains unclear. To explore the candidate genes and regulatory mechanism of soybean response to HT stress and flower development, here, the anthers of two previously identified HT-tolerant (JD21) and HT-sensitive (HD14) varieties were analyzed by RNA-seq. In total, 219 (172 upregulated and 47 downregulated), 660 (405 upregulated and 255 downregulated), and 4,854 (2,662 upregulated and 2,192 downregulated) differentially expressed genes (DEGs) were identified between JD21 anthers treated with HT stress vs. JD21 anthers in the natural field conditions (TJA vs. CJA), HD14 anthers treated with HT stress vs. HD14 anthers in the natural field conditions (THA vs. CHA), and JD21 vs. HD14 anthers treated with HT stress (TJA vs. THA), respectively. The results showed that there were more DEGs upregulated in JD21; this might be the reason why JD21 was more HT-resistant than the HT-sensitive variety HD14. GO annotation and KEGG enriched analysis showed that many DEGs are mainly involved in defense response, response to biological stimuli, auxin-activated signaling pathway, plant hormone signal transduction, MAPK signaling pathway-plant, starch and sucrose metabolism, etc. The conjoint analysis of RNA-seq and previous iTRAQ results found that there were 1, 24, and 54 common DEGs/DAPs showing the same expression pattern and 1, 2, and 13 common DEGs/DAPs showing the opposite pattern between TJA vs. CJA, THA vs. CHA, and TJA vs. THA at the protein and gene level, respectively, among which HSPs, transcription factor, GSTU, and other DEGs/DAPs participated in the response to HT stress and flower development. Notably, the qRT-PCR analysis and physiological index change results coincided with the sequencing results of RNA-seq and iTRAQ. In conclusion, the HT-tolerant cultivar performed better under stress than the HT-sensitive cultivar through modulation of HSP family proteins and transcription factors, and by keeping key metabolic pathways such as plant hormone signal transduction normal. This study provided important data and some key candidate genes to better study the effect and molecular basis of HT on anther in soybean at a transcription and translation level.
High resolution millimeter-wave imaging radar using inline Tx/Rx antennas (2008)

Broadband, high resolution millimeter-wave imaging radar using inline transmitting (Tx) and receiving (Rx) antennas is proposed. The imaging radar is composed of dielectric aspheric lens and inline Tx/Rx antennas located both in the axis of lens. As Tx/Rx antennas, antipodal Fermi antenna is employed. Basic characteristics of the proposed imaging radar including the effect of offset of Tx/Rx antennas is discussed by using FDTD analysis and experiments, in which high isolation between Tx/Rx antennas is obtained. Imaging of three objects is performed using frequency range of 35 GHz to 70 GHz showing high resolution less than 8 mm and clear image.
Autonomic Modulation for Cardiovascular Disease (2020)

Dysfunction of the autonomic nervous system has been implicated in the pathogenesis of cardiovascular disease, including congestive heart failure and cardiac arrhythmias. Despite advances in the medical and surgical management of these entities, progression of disease persists as does the risk for sudden cardiac death. With improved knowledge of the dynamic relationships between the nervous system and heart, neuromodulatory techniques such as cardiac sympathetic denervation and vagal nerve stimulation (VNS) have emerged as possible therapeutic approaches for the management of these disorders. In this review, we present the structure and function of the cardiac nervous system and the remodeling that occurs in disease states, emphasizing the concept of increased sympathoexcitation and reduced parasympathetic tone. We review preclinical evidence for vagal nerve stimulation, and early results of clinical trials in the setting of congestive heart failure. Vagal nerve stimulation, and other neuromodulatory techniques, may improve the management of cardiovascular disorders, and warrant further study.
Toward Environmentally Friendly Lithium Sulfur Batteries: Probing the Role of Electrode Design in MoS2-Containing Li–S Batteries with a Green Electrolyte (2019)

While lithium sulfur batteries (Li–S) hold promise as future high energy density low cost energy storage systems, barriers to implementation include low sulfur loading, limited cycle life, and the use of toxic electrolyte solvents. A comprehensive study of Li–S cells in the environmentally benign di(propylene glycol) dimethyl ether (DPGDME)-based electrolyte, using as-prepared MoS2 nanosheets derived from a facile aqueous microwave synthesis as polysulfide trapping agents, is reported herein for the first time. Conventional coated foil electrodes and binder-free electrodes (BFEs) with various structures are systematically generated and tested to correlate electrode design with the resulting electrochemical behavior. Significantly improved Li–S electrochemistry is demonstrated through the synergy of MoS2 chemistry and binder-free electrode engineering. In the coating configuration, the MoS2-containing cell evinced better rate performance and more stable cyclability than the cell without MoS2. In comparison with the coating counterparts, the BFE cells exhibited excellent cycle stability and superior rate capability (10-fold capacities and energy density per electrode weight with 20% higher retention rate) despite 2X higher areal sulfur loading. The BFE cell improvement can be attributed to the synergistic effect of the i) interconnected macroporous structure of CNT interlayers, providing a conductive framework, and ii) the efficient polysulfide trapping by the MoS2 nanosheets.
Systematic analysis of 18F-FDG PET and metabolism, proliferation and hypoxia markers for classification of head and neck tumors (2014)

With a select set of IHC markers representing cellular processes of metabolism, proliferation, hypoxia and perfusion, one can reliably distinguish between HNC tumor lines. Addition of 18F-FDG PET improves classification accuracy of IHC to a significant yet minor degree. These results may form a basis for development of tumor characterization models for treatment allocation purposes.
Phenotype, ontogeny, and repertoire of CD4-CD8- T cell receptor alpha beta + thymocytes. Variable influence of self-antigens on T cell receptor V beta usage (1991)

We have characterized CD4-CD8- double negative (DN) thymocytes that express TCR-alpha beta and represent a minor thymocyte subpopulation expressing a markedly skewed TCR repertoire. We found that DN TCR-alpha beta + thymocytes resemble mature T cells in that they (a) are phenotypically CD2hiCD5hiQa2+HSA-, (b) appear late in ontogeny, and (c) are susceptible to cyclosporin A-induced maturation arrest. In addition, we found that DNA sequences 5' to the CD8 alpha gene were demethylated relative to their germline state, suggesting that DN TCR-alpha beta + thymocytes are derived from cells that had at one time expressed their CD8 alpha gene locus. Because DN TCR-alpha beta + thymocytes are known to express an unusual TCR repertoire with significant overexpression of V beta 8, we were interested in examining the possible role played by self-Ag in shaping their TCR repertoire. It has been suggested that DN TCR-alpha beta + thymocytes are derived from potentially self-reactive thymocytes that have escaped clonal deletion by down-regulating their surface expression of CD4 and/or CD8 determinants. However, apparently inconsistent with such an hypothesis, we found that the frequency of DN thymocytes expressing various anti-self TCR (V beta 6, V beta 8.1, V beta 11, V beta 17a) were not increased in strains expressing their putative self-Ag, but instead were either unaffected or significantly reduced in those strains. With regard to V beta 8 expression among DN TCR-alpha beta + thymocytes, V beta 8 overexpression in DN TCR-alpha beta + thymocytes appeared to be independent of, and superimposed on, the developmental appearance of the basic DN thymocyte repertoire. Even though V beta 8 overexpression appeared to be generated by a mechanism distinct from that generating the rest of the DN TCR-alpha beta + thymocyte repertoire, we found that super-Ag against which V beta 8 TCR react introduced into the neonatal differentiation environment also significantly reduced, rather than increased, the frequency of DN TCR-alpha beta + V beta 8+ thymocytes. Thus, the present study is consistent with DN TCR-alpha beta + thymocytes being mature cells derived from CD8+ precursors, and documents that their TCR repertoire can be influenced, at least negatively, by either self-Ag or Ag introduced into the neonatal differentiation environment. However, we found no evidence to support the hypothesis that DN TCR-alpha beta + thymocytes are enriched in cells expressing TCR reactive against self-Ag.
Impact of high-NA half-field to full-field overlay performance by Monte Carlo simulations (2023)

In an earlier publication, we evaluated simulation methods to explore overlay performance implications when a highNumerical Aperture (high-NA) Extreme Ultra Violet (EUV) exposure is mixed and matched with a 0.33NA EUV full field exposure. The present contribution goes beyond the method description and aims to quantify the overlay performance impact in such a mix and match case, giving insight into the influence of different sets of overlay corrections. To this aim, the Mont Carlo engine has been updated to accommodate overlay corrections with up to 57 parameters. Since high-NA EUV is not yet available, typical overlay correction terms from IBM’s 0.33NA EUV tool have been used to approximate a realistic image placement error fingerprint for our simulations. As a result, we demonstrate the benefit of various overlay correction sets, and the detrimental effect when using different masks for top and bottom half fields. Such information can also help to infer design layout placement decisions to avoid hot spot regions.
Ultra-compact nanophotonic devices designed by computational metamaterials (2017)

Computationally designed metamaterials are used to realize a number of novel nanophotonic devices. We report on the development of ultra-compact on-chip polarization rotators, waveguide cloaks and all optical modulators made possible by the use of a non-linear optimization algorithm.
Generation of hen egg lysozyme‐specific and major histocompatibility complex class I‐restricted cytolytic T lymphocytes: recognition of cytosolic and secreted antigen expressed by transfected cells (1990)

Abstract Syngeneic cells exogenously supplied with hen egg lysozyme (HEL) or endogenously synthesizing HEL were used as antigen‐presenting cells to induce major histocompatibility complex class I‐restricted cytotoxic T lymphocytes (CTL). Immunization of C57BL/6 mice followed by repeated stimulation of their splenocytes in vitro with trypsinized HEL peptides led to the generation of CTL lines specific for trypsinized HEL peptides and restricted by H‐2K b . Immunization of C3H mice with a mixture of soluble native HEL and irradiated syngeneic spleen cells followed by in vitro stimulation of immune spleen cells with soluble HEL could in a few cases result in HEL‐specific CTL able to kill syngeneic transfectant L cells secreting HEL (HELs) or expressing cytosol‐targeted HEL (HELc). The use of HELs or HELc transfectant L cells as in vivo and in vitro immunogens was a potent way for eliciting HEL‐specific polyclonal CTL. These CTL and two CD8 + clones were found to be H‐2K k restricted and specific for the 1‐17 N‐terminal HEL peptide. In addition, the anti‐HEL CTL could also exhibit a significant cross‐reactivity against unsensitized and HEL‐untransfected targets expressing the K restriction element. This cross‐reactivity was likely due to recognition of unidentified HEL mimicking peptides (self‐derived ?) presented by the MHC class I (H‐2K b or H‐2K k ) molecule used as the restriction element for the specific recognition of HEL. The CTL raised after immunization with HELs or HELc transfectant cells were found to recognize both the HELs and HELc transfectant cells even though HEL was not detected in the latter after a 2‐ or 5‐min radiolabeling pulse. Recognition of both HELs and HELc transfectant cells by a given CTL clone suggests that HEL subjected to two separate processing pathways, each depending on the initial subcellular localization, can ensure the generation of similar MHC class I peptide complexes.
Effects of metallisation and bondfeets in 3.3 kV free-wheeling diodes at surge current conditions (2008)

The ruggedness of power electronic systems is of high significance. Diodes are one decisive part. In this paper we investigated the influence of the metallisation and bond wires on the temperature, surge current and current inhomogenisation of silicon free-wheeling diodes by simulation. Thereby, the dependencies of metallisation thickness and material as well as bond foot contact area and pitch were analysed and discussed.
100 Gb/s DAC-less and DSP-free Transmitters using GeSi EAMs for Short-Reach Optical Interconnects (2018)

We present single-lane 100-Gb/s NRZ, electrical duobinary and PAM-4 transmitters using silicon photonics GeSi electro-absorption modulators.No DSP, DAC or traveling-wave structures are required, enabling compact and low-power transceivers for data center interconnects.
Cas9 RNP transfection by vapor nanobubble photoporation for ex vivo cell engineering (2021)

The CRISPR-Cas9 technology represents a powerful tool for genome engineering in eukaryotic cells, advancing both fundamental research and therapeutic strategies. Despite the enormous potential of the technology, efficient and direct intracellular delivery of Cas9 ribonucleoprotein (RNP) complexes in target cells poses a significant hurdle, especially in refractive primary cells. In the present work, vapor nanobubble (VNB) photoporation was explored for Cas9 RNP transfection in a variety of cell types. Proof of concept was first demonstrated in H1299-EGFP cells, before proceeding to hard-to-transfect stem cells and T cells. Gene knock-out levels over 80% and up to 60% were obtained for H1299 cells and mesenchymal stem cells, respectively. In these cell types, the unique possibility of VNB photoporation to knock out genes according to user-defined spatial patterns was demonstrated as well. Next, effective targeting of the programmed cell death 1 receptor and Wiskott-Aldrich syndrome gene in primary human T cells was demonstrated, reaching gene knock-out levels of 25% and 34%, respectively. With a throughput of >200,000 T cells per second, VNB photoporation is a scalable and versatile intracellular delivery method that holds great promise for CRISPR-Cas9-mediated <i>ex vivo</i> engineering of cell therapy products.