736 PRO-INFLAMMATORY AND MITOGENIC ACTIVITY OF PERIPROSTATIC ADIPOSE TISSUE IN PROSTATE CANCER (2011)

You have accessJournal of UrologyProstate Cancer: Basic Research1 Apr 2011736 PRO-INFLAMMATORY AND MITOGENIC ACTIVITY OF PERIPROSTATIC ADIPOSE TISSUE IN PROSTATE CANCER David S. Finley, Colette Galet, Joyce Yamashiro, Evelyn Kono, Chau Tran, Alan Pantuck, Jean Dekernion, William Aronson, and Robert Reiter David S. FinleyDavid S. Finley Los Angeles, CA More articles by this author , Colette GaletColette Galet Los Angeles, CA More articles by this author , Joyce YamashiroJoyce Yamashiro Los Angeles, CA More articles by this author , Evelyn KonoEvelyn Kono Los Angeles, CA More articles by this author , Chau TranChau Tran Los Angeles, CA More articles by this author , Alan PantuckAlan Pantuck Los Angeles, CA More articles by this author , Jean DekernionJean Dekernion Los Angeles, CA More articles by this author , William AronsonWilliam Aronson Los Angeles, CA More articles by this author , and Robert ReiterRobert Reiter Los Angeles, CA More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.1705AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Obesity has been characterized as a state of chronic, low-grade inflammation. There is evidence linking obesity with prostate cancer (CaP) aggressiveness. Visceral adipose tissue has recently been recognized as a bioactive endocrine organ that secretes a host of cytokines and growth factors, many of which are pro-inflammatory and may modulate the tumor microenvironment. The objective of this pilot study was to characterize inflammatory gene expression in various adipose tissue depots and its effect on prostate cancer cell growth. METHODS Periprostatic (PAT) and subcutaneous adipose control tissue (SAT) were harvested from patients with prostate cancer undergoing radical prostatectomy or from control patients with BPH undergoing simple prostatectomy. Adipose was directly analyzed by inflammatory PCR array (n=8) or used to generate conditioned medium (CM) for cell proliferation assays using a 22Rv1 prostate cancer cell line (n=9). RESULTS A host of inflammatory genes (e.g. IL8RA, ILRAB, CXCL2, CCL8) were modestly upregulated (5-6X) in high-grade (Gleason 9) CaP associated-PAT compared with SAT (Figure 1). Several genes including CCL21 and TOLLIP were upregulated 5.9 and 4.8X, respectively, in high-grade CaP associated-PAT compared with BPH associated-PAT while CCL2,CCL4 and CXCL1-3 were down-regulated 4-9.9X, respectively. In both BRDU and MTS assays, high-grade CaP associated PAT-CM stimulated 22Rv1 cell proliferation more than SAT, low-grade or BPH-associated PAT (Figure 2). CONCLUSIONS In this pilot study, PAT from CaP patients demonstrated upregulation of pro-inflammatory gene expression. Conditioned media generated from cancer associated PAT stimulated 22Rv1 cell growth and proliferation more than BPH associated PAT or SAT. In addition, the magnitude of the effect was greatest for high grade cancer associated PAT. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e295-e296 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information David S. Finley Los Angeles, CA More articles by this author Colette Galet Los Angeles, CA More articles by this author Joyce Yamashiro Los Angeles, CA More articles by this author Evelyn Kono Los Angeles, CA More articles by this author Chau Tran Los Angeles, CA More articles by this author Alan Pantuck Los Angeles, CA More articles by this author Jean Dekernion Los Angeles, CA More articles by this author William Aronson Los Angeles, CA More articles by this author Robert Reiter Los Angeles, CA More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...