gebhart/patrepeval huggingface dataset:
Adenosine A3 agonists reverse neuropathic pain via T cell–mediated production of IL-10 (2021)

The A3 adenosine receptor (A3AR) has emerged as a therapeutic target with A3AR agonists to tackle the global challenge of neuropathic pain, and investigation into its mode of action is essential for ongoing clinical development. Immune cell A3ARs, and their activation during pathology, modulate cytokine release. Thus, the use of immune cells as a cellular substrate for the pharmacological action of A3AR agonists is enticing, but unknown. The present study discovered that Rag-KO mice lacking T and B cells, as compared with WT mice, are insensitive to the anti-allodynic effects of A3AR agonists. Similar findings were observed in interleukin-10 and interleukin-10 receptor knockout mice. Adoptive transfer of CD4+ T cells from WT mice infiltrated the dorsal root ganglion (DRG) and restored A3AR agonist-mediated anti-allodynia in Rag-KO mice. CD4+ T cells from Adora3-KO or Il10-KO mice did not. Transfer of CD4+ T cells from WT mice, but not Il10-KO mice, into Il10-KO mice or Adora3-KO mice fully reinstated the anti-allodynic effects of A3AR activation. Notably, A3AR agonism reduced DRG neuron excitability when cocultured with CD4+ T cells in an IL-10-dependent manner. A3AR action on CD4+ T cells infiltrated in the DRG decreased phosphorylation of GluN2B-containing N-methyl-D-aspartate receptors at Tyr1472, a modification associated with regulating neuronal hypersensitivity. Our findings establish that activation of A3AR on CD4+ T cells to release IL-10 is required and sufficient evidence for the use of A3AR agonists as therapeutics.
Hybrid solar lighting distribution systems and components (2023)

A hybrid solar lighting distribution system and components having at least one hybrid solar concentrator, at least one fiber receiver, at least one hybrid luminaire, and a light distribution system operably connected to each hybrid solar concentrator and each hybrid luminaire. A controller operates all components.
Model based segmentation of motion fields in compressed video sequences using partition projection and relaxation (2005)

In the context of visual signal analysis for media adaptation, this paper presents a stochastic method for sementation of motion vector fields or as known as displacement fields in compressed videos. The addressed field sequence is analysed and partitioned to temporally associated, motion-semantic regions by three analysis steps. The global motion based reliability analysis assesses the reliability extent of each encoded displacement vector. Upon the measure array, an initial partition is approximated by the region displacement prediction. The subsequent relaxation procedure optimises the partition shape, coverage, and location using the <i>stochastic motion coherency</i> model and the second-order random field based contour smoothness. The visually convincing results are demonstrated from the standard sequences.
Human Action Recognition from Boosted Pose Estimation (2010)

This paper presents a unified framework for recognizing human action in video using human pose estimation. Due to high variation of human appearance and noisy context background, accurate human pose analysis is hard to achieve and rarely employed for the task of action recognition. In our approach, we take advantage of the current success of human detection and view invariability of local feature-based approach to design a pose-based action recognition system. We begin with a frame-wise human detection step to initialize the search space for human local parts, then integrate the detected parts into human kinematic structure using a tree structural graphical model. The final human articulation configuration is eventually used to infer the action class being performed based on each single part behavior and the overall structure variation. In our work, we also show that even with imprecise pose estimation, accurate action recognition can still be achieved based on informative clues from the overall pose part configuration. The promising results obtained from action recognition benchmark have proven our proposed framework is comparable to the existing state-of-the-art action recognition algorithms.
A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis (2022)

Abstract The OsTIR1/auxin-inducible degron (AID) system allows selective protein degradation upon exposure to the phytohormone auxin. However, this technology does not allow to study the effect of acute protein depletion selectively in one phase of the cell cycle. Here, we report a new AID system to Regulate OsTIR1 Levels based on the Cell Cycle Status (ROLECCS) for phase-specific target proteolysis. Finally, we applied the ROLECCS technology to show that the tumor suppressor TP53 plays a S/G2-specific role in suppression of micronuclei accumulation. This new tool allows the analysis of different protein functions during cell cycle progression with unprecedented temporal resolution.
Bacterial RNAP Inhibitors: Synthesis and Evaluation of Prodrugs of Aryl‐ureidothiophene‐carboxylic acids (2017)

Abstract Recently, aryl‐ureidothiophene carboxylates were shown to inhibit the RNA polymerase and reduce the growth of Gram positive bacteria. We now aimed to extend the efficacy spectrum and address presumed permeation deficiencies of these agents by applying different prodrug‐approaches. Chemical syntheses yielded 12 prodrugs of three different categories featuring neutral, cationic and Fe III ‐coordinating properties. Their promising characteristics regarding stabilities, enzyme‐activation and iron coordination performance encouraged their study in growth inhibition assays of different Gram positive and Gram negative bacterial strains. These studies pointed to a positively charged, choline‐like motif being beneficial for these compounds especially for Gram‐negative bacterial cell growth inhibition.
The simple way of lung membrane diffusion capacity and alveolar volume evaluation (2017)

<b>Background:</b> We found earlier [Babarskov E. at al. ERJ 2013; 42, suppl. 57: 274s] the relationship beetwen integral carbon monoxide (CO) concentration C<sub>m</sub>(ppm) in exhaled air, C<sub>e</sub><sub></sub>(ppm) in inhaled air and lung membrane diffision capacity D<sub>m</sub>(mL/min/mmHg), alveolar volume V<sub>a</sub>(L), total dead space voloume (instrument+anatomic) V<sub>d</sub>(L), taking into account major spirometric parameters: tidal volume V<sub>t</sub><sub></sub>(L) and respiratory cycle time T<sub>c</sub>(s); at different breathing regimes. <b>Aim:</b> To calculate D<sub>m</sub> and V<sub>a</sub>, using known CO concentration values. <b>Methods:</b> Calculation of exhaled CO concentration and experimental data analysis for healthy volunteer with predicted values: D<sub>m</sub>=60.94; V<sub>a</sub>=6.83. Solution of inverse task (the system of 2 algebraic equations), that is calculation of real D<sub>m</sub> and V<sub>a</sub> values, taking intu account experimental data. <b>Results:</b> At C<sub>e</sub>=0.42 and V<sub>d</sub>=0.3 in experiment for spontanios breathing (V<sub>t</sub>=1.0; T<sub>c</sub>=6) C<sub>ms</sub>=1.44. For deep forsed breathing (V<sub>t</sub>=5.0; T<sub>c</sub>=6) C<sub>mf</sub>=1.17 and after breath holding during time T: 5, 10, 15, 25s; C<sub>mt</sub>=1.75, 1.99, 2.11, 2.24, accordingly. Calculated values of CO concentration deviated from experimental values not more than 10%. The solution of inverse task is defined by 2 dimensionless parameters: R<sub>s</sub>=(C<sub>mf</sub>-C<sub>e</sub>)/(C<sub>ms</sub>-C<sub>e</sub>); R<sub>t</sub>=(C<sub>mt</sub>-C<sub>e</sub>)/(C<sub>mf</sub>-C<sub>e</sub>). Values of D<sub>m</sub> and V<sub>a</sub><sub></sub>, calculated using experimental data, differ from predicted velues not more than +15% and -5%, accordingly. <b>Conclusion:</b> Our findings provide a basis for development of new method for D<sub>m</sub> and V<sub>a</sub> evaluation without using of balloons with test gas mixtures.
After the Irish: an anthology of poetic translation (2011)

Gregory A. Schirmer, Cork, Cork University Press, 2009, 500 pp., €85.00 (hardback), ISBN 978-1-85918-438-7 Translation from Irish is less an adjunct to anglophone Irish poetry than a condition of i...
SOOTING LIMITS OF DIFFUSION FLAMES WITH OXYGEN- ENRICHED AIR AND DILUTED FUEL (2003)

Oxygen-enhanced combustion permits certain benefits and flexibility that are not otherwise available in the design of practical combustors, as discussed by Baukal. The cost of pure and enriched oxygen has declined to the point that oxygen-enhanced combustion is preferable to combustion in air for many applications. Carbon sequestration is greatly facilitated by oxygen enrichment because nitrogen can be eliminated from the product stream. For example, when natural gas (or natural gas diluted with CO2) is burned in pure oxygen, the only significant products are water and CO2. Oxygen-enhanced combustion also has important implications for soot formation, as explored in this work. We propose that soot inception in nonpremixed flames requires a region where C/O ratio, temperature, and residence time are above certain critical values. Soot does not form at low temperatures, with the threshold in nonpremixed flames ranging from about 1250-1650 K, a temperature referred to here as the critical temperature for soot inception, Tc. Soot inception also can be suppressed when residence time is short (equivalently, when the strain rate in counterflow flames is high). Soot induction times of 0.8-15 ms were reported by Tesner and Shurupov for acetylene/nitrogen mixtures at 1473 K. Burner stabilized spherical microgravity flames are employed in this work for two main reasons. First, this configuration offers unrestricted control over convection direction. Second, in steady state these flames are strain-free and thus can yield intrinsic sooting limits in diffusion flames, similar to the way past work in premixed flames has provided intrinsic values of C/O ratio associated with soot inception limits.
STIMULATION OF PHOSPHATIDIC ACID AND PHOSPHATIDYLINOSITOL LABELING IN LUTEAL CELLS BY LUTEINIZING HORMONE RELEASING HORMONE (1983)

Luteinizing hormone-releasing hormone (LHRH) causes a rapid and marked increase of [32P]orthophosphate incorporation into phosphatidylinositol (PI) and phosphatidic acid (PA) in rat luteal cells in culture. The neurohormone exerts its stimulatory effect at an ED50 value of approximately 15 nM. Human chorionic gonadotropin (hCG) has no effect alone and does not interfere with the LHRH-induced PA-PI labeling. The rapidity and the specificity of the effect of LHRH suggest that the stimulation of the PA-PI cycle may well serve as a potent transducing mechanism responsible for the direct action of LHRH and its agonists at the ovarian level.
Simple fabrication of zinc oxide nanostructures (2008)

A simple and low cost methodology for fabricating one and two dimensional periodic ZnO nanostructures utilizing surface relief patterns on azopolymer films as templates is demonstrated.
Narrow Complex Tachycardia with VA Block: Diagnostic and Therapeutic Implications (1998)

To review our experience with cases of narrow complex tachycardia with VA block, highlighting the difficulties in the differential diagnosis, and the therapeutic implications. Prior reports of patients with narrow complex tachycardia with VA block consist of isolated case reports. The differential diagnosis of this disorder includes: automatic junctional tachycardia, AV nodal reentry with final upper common pathway block, concealed nodofascicular (ventricular) pathway, and intra-Hissian reentry. Between June 1994 and January 1996, six patients with narrow complex tachycardia with episodes of ventriculoatrial block were referred for evaluation. All six patients underwent attempted radiofrequency ablation of the putative arrhythmic site. Three of six patients had evidence suggestive of a nodofascicular tract. Intermittent antegrade conduction over a left-sided nodofascicular tract was present in two patients and the diagnosis of a concealed nodofascicular was made in the third patient after ruling out other tachycardia mechanisms. Two patients had automatic junctional tachycardia, and one patient had atrioventricular nodal reentry with proximal common pathway block. Attempted ablation in the posterior and mid-septum was unsuccessful in patients with nodofascicular tachycardia. In contrast, those with atrioventricular nodal reentry and automatic junctional tachycardia readily responded to ablation. The presence of a nodofascicular tachycardia should be suspected if: (1) intermittent antegrade preexcitation is recorded, (2) the tachycardia can be initiated with a single atrial premature producing two ventricular complexes, and (3) a single ventricular extrastimulus initiates SVT without a retrograde His deflection. The presence of a nodofascicular pathway is common in patients with narrow complex tachycardia and VA block. Unlike AV nodal reentry and automatic junctional tachycardia, the response to ablation is poor.
The luminescent HiBiT peptide enables selective quantitation of G protein–coupled receptor ligand engagement and internalization in living cells (2020)

G protein-coupled receptors (GPCRs) are prominent targets to new therapeutics for a range of diseases. Comprehensive assessments of their cellular interactions with bioactive compounds, particularly in a kinetic format, are imperative to the development of drugs with improved efficacy. Hence, we developed complementary cellular assays that enable equilibrium and real-time analyses of GPCR ligand engagement and consequent activation, measured as receptor internalization. These assays utilize GPCRs genetically fused to an N-terminal HiBiT peptide (1.3 kDa), which produces bright luminescence upon high-affinity complementation with LgBiT, an 18-kDa subunit derived from NanoLuc. The cell impermeability of LgBiT limits signal detection to the cell surface and enables measurements of ligand-induced internalization through changes in cell-surface receptor density. In addition, bioluminescent resonance energy transfer is used to quantify dynamic interactions between ligands and their cognate HiBiT-tagged GPCRs through competitive binding with fluorescent tracers. The sensitivity and dynamic range of these assays benefit from the specificity of bioluminescent resonance energy transfer and the high signal intensity of HiBiT/LgBiT without background luminescence from receptors present in intracellular compartments. These features allow analyses of challenging interactions having low selectivity or affinity and enable studies using endogenously tagged receptors. Using the β-adrenergic receptor family as a model, we demonstrate the versatility of these assays by utilizing the same HiBiT construct in analyses of multiple aspects of GPCR pharmacology. We anticipate that this combination of target engagement and proximal functional readout will prove useful to the study of other GPCR families and the development of new therapeutics.
a research platform for a smart-blade wind generation system (2008)

A research platform, which is designed for a novel smart-blade wind turbine system, is presented. The proposed platform, which allows for the integration of different renewable sources, consists of a smart-blade wind turbine, a permanent magnet (PM) generator, a rectifier, a hybrid super-capacitor/battery energy buffer, and a boost and bi-directional converter. The PM generator, designed for the power profile of the smart-blade turbine, is driven by an induction motor drive to emulate the wind conditions. The research platform is implemented in a Simulink/MATLAB environment with a dSPACE interface for real time control. It has a controller for peak power point tracking (PPPT) and a user interface for real time metering, and is expected to facilitate rapid prototyping and energy related research.
0.622-8.0 Gbps 150 mW serial IO macrocell with fully flexible preemphasis and equalization (2004)

This paper presents a 622 Mbps to 8 Gbps transceiver in standard 0.13 /spl mu/m CMOS technology. Each receiver and transmitter macrocell has its dedicated clock multiplication unit (CMU) and clock/data recovery unit (CDR), providing simultaneous multi-rate operation for multiple lanes on a chip. The transmitter and receiver front-end use direct 4:1 multiplex and 1:4 demultiplexing, using multiple-phase quarter-rate clocks. An automatic phase offset cancellation scheme is used to eliminate the phase mismatch of the multiple clock phases. Each transceiver occupies an active area of less than 0.4 mm/sup 2/ and consumes 150 mW at maximum speed.
Isolation of a Lipopolysaccharide Mutant of Neisseria gonorrhoeae: An Analysis of the Antigenic and Biologic Differences (1982)

Analysis of the surface constituents of a pyocin 611 131-resistant variant of strain no. JW-31 of Neisseria gonorrhoeae revealed substantial differences in the lipopolysaccharide (LPS) but not changes in the auxotype or outer-membrane proteins. Immunodiffusion and an enzyme-linked immunosorbent assay showed that the variant strain (no. JW-31R) lost both the LPS serotype and the variable antigens while retaining at least a portion of the common determinant. The use of monoclonal antibody indicated that LPSs from strain no. JW-31R and pyocin 611 131-resistant strains of other LPS serotypes lack a D-galactosaminyl-D-galactopyranosyl-D-glucose moiety. The LPS-derived polysaccharide from strain no. JW-31 binds to wheat-germ lectin in precipitin and inhibition systems, whereas the JW-31R polysaccharide exhibits a markedly reduced affinity. In the presence of normal human serum, 99% of strain no. JW-31R was killed within 20 min and strain no. JW-31 was not.
Metagenomic assembly and bin3C clustering result for a healthy human faecal microbiome transplant donor (2019)

Metagenomic WGS assembly and Hi-C deconvolution of a healthy human faecal microbiome transplant donor. Metagenomic assembly was produced using Spades (v3.13.1). Extracted MAGs were produced using bin3C (v0.3.3) and QC'd using CheckM (v1.0.18).
Cancellation of Unnatural Reaction Torque in Variable-Gear-Ratio (2012)

Variable-gear-ratio steering, also known as active steering, is an advanced steering technology which enhances the driver’s comfort and vehicle operability. However, one big problem, namely the unnatural reaction torque created by the variable actuator, restricts the further practical-use of the variable-gear-ratio steering. This paper proposes a control method to cancel the unnatural torque using a simple concept called friction relocation. Effectiveness of the method is experimentally confirmed using a hardware-in-the-loop simulator.
Sharing Policies in Multiuser Privacy Scenarios (2017)

Social network services (SNSs) enable users to conveniently share personal information. Often, the information shared concerns other people, especially other members of the SNS. In such situations, two or more people can have conflicting privacy preferences; thus, an appropriate sharing policy may not be apparent. We identify such situations as multiuser privacy scenarios . Current approaches propose finding a sharing policy through preference aggregation. However, studies suggest that users feel more confident in their decisions regarding sharing when they know the reasons behind each other’s preferences. The goals of this paper are (1) understanding how people decide the appropriate sharing policy in multiuser scenarios where arguments are employed, and (2) developing a computational model to predict an appropriate sharing policy for a given scenario. We report on a study that involved a survey of 988 Amazon Mechanical Turk (MTurk) users about a variety of multiuser scenarios and the optimal sharing policy for each scenario. Our evaluation of the participants’ responses reveals that contextual factors, user preferences, and arguments influence the optimal sharing policy in a multiuser scenario. We develop and evaluate an inference model that predicts the optimal sharing policy given the three types of features. We analyze the predictions of our inference model to uncover potential scenario types that lead to incorrect predictions, and to enhance our understanding of when multiuser scenarios are more or less prone to dispute.
Polarization invariant plasmonic nanostructures for sensing applications (2017)

Optics-based sensing platform working under unpolarized light illumination is of practical importance in the sensing applications. For this reason, sensing platforms based on localized surface plasmons are preferred to their integrated optics counterparts for their simple mode excitation and inexpensive implementation. However, their optical response under unpolarized light excitation is typically weak due to their strong polarization dependence. Herein, the role of rotational symmetry for realizing robust sensing platform exhibiting strong optical contrast and high sensitivity is explored. Specifically, gammadion and star-shaped gold nanostructures with different internal and external rotational symmetries are fabricated and studied in detail, from which their mode characteristics are demonstrated as superposition of their constituent longitudinal plasmons that are in conductive coupling with each other. We demonstrate that introducing and increasing internal rotational symmetry would lead to the enhancement in optical contrast up to ~3x under unpolarized light illumination. Finally, we compare the sensing performances of rotationally symmetric gold nanostructures with a more rigorous figure-of-merit based on sensitivity, Q-factor, and spectral contrast.
ChemInform Abstract: Cyclopropanation Mediated by Zinc Organometallics (2009)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
A dry wafer-reconstitution process with zero insertion force by embedded alignment guide tabs (2012)

We demonstrate embedding of chiplets into wafers to bypass the monolithic integration challenges of combining micro-mechanical devices with active circuitry. Dry alignment is provided by passive guide tabs, which are created through polysilicon refill of frontside etched trenches. The guide tabs are compatible with further processing on the wafer. Precision chiplets are created by through-wafer etching. After chiplet drop-in, optical detection provides feedback of chiplet jamming, and horizontal driving of the chiplets finishes the zero insertion force assembly process. We demonstrate less than 2.5 µm of chiplet to substrate alignment error. Theoretical scaling of alignment accuracy reaches numbers comparable to photolithography, allowing high density interconnects for heterogeneous integration without precision manipulators.