gebhart/patrepeval huggingface dataset:
Mutational landscape of uterine and ovarian carcinosarcomas implicates histone genes in epithelial–mesenchymal transition (2016)

Carcinosarcomas (CSs) of the uterus and ovary are highly aggressive neoplasms containing both carcinomatous and sarcomatous elements. We analyzed the mutational landscape of 68 uterine and ovarian CSs by whole-exome sequencing. We also performed multiregion whole-exome sequencing comprising two carcinoma and sarcoma samples from six tumors to resolve their evolutionary histories. The results demonstrated that carcinomatous and sarcomatous elements derive from a common precursor having mutations typical of carcinomas. In addition to mutations in cancer genes previously identified in uterine and ovarian carcinomas such as TP53, PIK3CA, PPP2R1A, KRAS, PTEN, CHD4, and BCOR, we found an excess of mutations in genes encoding histone H2A and H2B, as well as significant amplification of the segment of chromosome 6p harboring the histone gene cluster containing these genes. We also found frequent deletions of the genes TP53 and MBD3 (a member with CHD4 of the nucleosome remodeling deacetylase complex) and frequent amplification of chromosome segments containing the genes PIK3CA, TERT, and MYC Stable transgenic expression of H2A and H2B in a uterine serous carcinoma cell line demonstrated that mutant, but not wild-type, histones increased expression of markers of epithelial-mesenchymal transition (EMT) as well as tumor migratory and invasive properties, suggesting a role in sarcomatous transformation. Comparison of the phylogenetic relationships of carcinomatous and sarcomatous elements of the same tumors demonstrated separate lineages leading to these two components. These findings define the genetic landscape of CSs and suggest therapeutic targets for these highly aggressive neoplasms.
Frontiers in Clinical Drug Research: Anti-Infectives (2016)

Nosocomial infections caused by multidrug-resistant strains increase morbidity and mortality in hospitalized patients, prolong hospitalization, and they have significant negative economic impact. Currently, nosocomial infections are included in a broader concept as healthcare-associated infections. These are caused by a wide variety of pathogens, typically Staphylococci, Enterococci, Pseudomonas aeruginosa, Klebsiella spp., Escherichia coli, etc. Certain fungal agents, e.g., yeasts of Candida genus and aspergilla, are also associated with these infections. The situation is further complicated by the development of resistance in the gram-positive cocci such as staphylococci or enterococci to second-line antibiotics used for the treatment of gramnegative bacteria and resistant gram-positive bacteria. Pathogenesis of mentioned infections plays a key role in forming biofilms on artificial surfaces.
Annihilation mechanism of V-shaped pits in c-GaN grown by hydride vapor-phase epitaxy (2019)

Abstract An evident issue in performing GaN growth along the c -direction is the generation of V-shaped pits on its surface. Here, we investigated the transition of the angle of facets forming a V-shaped pit via three-photon excitation photoluminescence (3PPL). The intermittent doping of Ge was introduced to GaN growth to observe the growth front. The size of the V-shaped pit composed of { <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" overflow="scroll"> <mml:mn>10</mml:mn> <mml:mover accent="true"> <mml:mn>1</mml:mn> <mml:mo stretchy="true">¯</mml:mo> </mml:mover> <mml:mn>2</mml:mn> </mml:math> } facets decreased along the growth direction, whereas that composed of { <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" overflow="scroll"> <mml:mn>10</mml:mn> <mml:mover accent="true"> <mml:mn>1</mml:mn> <mml:mo stretchy="true">¯</mml:mo> </mml:mover> <mml:mn>1</mml:mn> </mml:math> } facets, increased. Furthermore, planar growths of c -GaN and semipolar GaN having various surface orientations revealed that the V-shaped pit composed of { <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" overflow="scroll"> <mml:mn>10</mml:mn> <mml:mover accent="true"> <mml:mn>1</mml:mn> <mml:mo stretchy="true">¯</mml:mo> </mml:mover> <mml:mn>2</mml:mn> </mml:math> } was likely to annihilate rather than that of { <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" overflow="scroll"> <mml:mn>10</mml:mn> <mml:mover accent="true"> <mml:mn>1</mml:mn> <mml:mo stretchy="true">¯</mml:mo> </mml:mover> <mml:mn>1</mml:mn> </mml:math> } under the growth condition of N 2 carrier gas, which coincides with the result of 3PPL.
Electrochemical Capacitive Behaviour of Multiwalled Carbon Nanotubes Modified with Electropolymeric Films of Nickel Tetraaminophthalocyanine (2010)

Abstract Electrochemical capacitive behaviour of the electropolymeric nickel tetra‐aminophthalocyanine (poly‐NiTAPc) supported on multiwalled carbon nanotube (MWCNT) platform is described. From the data in 1 M H 2 SO 4 , the MWCNT‐poly‐NiTAPc exhibited superior capacitance (112 mF cm −2 or 777 F g −1 ). The supercapacitance of the MWCNT‐polyNiTAPc was found to be much better in 1 M H 2 SO 4 than in 1 M NaOH medium. Impedance data proved that most of the stored energy of the MWCNT‐poly‐NiTAPc could be accessible at high frequency (1.6 kHz). The electrode is capable of undergoing continuous charge‐discharge process for more than a 1000 cycles without any significant deterioration in the stability.
Nondegenerate, Transient Nonlinear Refraction of Indium Tin Oxide Excited at Epsilon-Near-Zero (2017)

We measure nondegenerate nonlinear refraction of ITO as we tune the excitation wavelength through Epsilon-Near-Zero (ENZ) for a fixed probing wavelength. Unlike the degenerate case, there is no observed peak in the nonlinearity at ENZ.
Parameter estimation of multiple pulse trains for illumination sensing (2010)

We consider illumination sensing in a light emitting diode (LED) lighting system based on frequency division multiplexing (FDM). In the FDM scheme, LEDs render periodical illumination pulses at different frequencies with prescribed duty cycles. The purpose of illumination sensing is to identify the illumination contributions of different LEDs at a target location, within a limited response time. With the use of a photosensor at the target location, the problem of interest is to estimate the amplitude of the sensor signal component due to each LED. In this paper, we propose a successive estimation approach exploiting higher order harmonics of the sensor signal. We show that a larger number of LEDs can be supported using the proposed approach, as compared to our previous approach using only the fundamental frequency component, at the same estimation error.
Picosecond single-mode pulse compression using a 1.3 μm Fabry–Perot laser diode, a dispersion-shifted fiber, and a grating monochromator (1989)

A single longitudinal mode optical pulse train with a full width at half maximum less than 7.2 ps was generated by compressing gain-switched pulses from a 1.3 μm multimode laser diode using a monochromator and a dispersion-shifted single-mode optical fiber.
GNSS/GPS Robustness for UAS (2016)

RFI (Radio Frequency Interference) caused by close proximity of electronics to the GNSS/GPS (Global Navigation Satellite System/Global Positioning System) antenna on a UAS (Unmanned Aircraft System) has the potential to degrade GNSS signals by raising the noise floor level. Many common electronics are unintentional radiators, and leak a certain degree of RFI into the surrounding area. An experiment to determine the presence of RFI on a UAS with some common UAS components was performed and is described in this paper. RTK (Real Time Kinematics) can be used to improve the accuracy of the UAS GNSS engine, and could potentially give position measurements that are accurate to within a few centimeters. However, RTK positioning is more complex than traditional code-based GNSS, and requires additional hardware. If RTK is to be used for navigational purposes on a UAS it is vital that performance can be guaranteed. A case study was performed to give an insight into what type of performance can be expected of an airborne RTK system, and to build upon and validate previous work. The ultimate goal of the work described in this paper was to provide a link between the two areas described above, and to examine how onboard RFI affects RTK on UAS. It is likely that RFI will have a significant impact on RTK measurements since RTK relies on the relatively frail carrier phase measurements. However, this link was never made due to time constraints, and so it remains unresolved.
Participation of vasoactive intestinal polypeptide in ovarian steroids production during the rat estrous cycle and in the development of estradiol valerate-induced polycystic ovary (2007)

Vasoactive intestinal polypeptide (VIP) stimulates estradiol and progesterone release from ovarian granulosa cells in vitro. Very little information is available as to the role VIP plays in the control of steroid secretion during reproductive cyclicity and in ovarian pathologies involving altered steroid secretion. In this study, we determined the involvement of VIP in regulating ovarian androgen and estradiol release during estrous cyclicity and estradiol valerate (EV)-induced polycystic ovarian development in rats. Our findings show that androgen and estradiol release from ovaries obtained during different stages of rat estrous cycle mimic cyclic changes in steroid release observed in vivo with maximal release occurring during late proestrus. VIP increased androgen release from ovaries of all cycle stages except late proestrus and estradiol release from all cycle stages. Increases in VIP-induced androgen and estradiol release were maximal at early proestrus. Inclusion of saturating concentrations of androstenedione increased magnitude of VIP-induced estradiol release at diestrus and estrus but not proestrus. Magnitude of VIP-induced androgen and estradiol release tended to be greater in the ovaries from EV-treated rats with polycystic ovary compared with estrous controls. At the tissue level, ovarian VIP concentration was cycle stage dependent with highest level seen in diestrus. Maximum concentration of VIP was found in EV-treated rats. Changes in VIP were inversely related to changes in ovarian nerve growth factor, a neuropeptide involved in ovarian androgen secretion. These results strongly suggest that intraovarian VIP participates in the control of estradiol secretion during the rat estrous cycle and possibly in the maintenance of increased ovarian estradiol secretory activity of EV-treated rats.
Advances in dendritic cell-based therapeutic vaccines for cervical cancer (2007)

Cervical cancer is the second leading cause of cancer death among women worldwide and remains an important health problem for women, especially in underserved and minority groups in industrially developed nations. Although radical surgery and radiotherapy represent effective modalities of treatment for invasive cervical cancer, up to 35% of these patients overall will develop recurrent/metastatic disease for which treatment results remain poor. Novel therapeutic strategies that are effective in reducing the risk of recurrence/metastatic disease are still needed desperately. Human papillomavirus (HPV) infection represents the most important risk factor for the development of cervical dysplasia and cervical cancer. Since HPVE6 and E7 oncoproteins are constantly expressed in these lesions, these foreign proteins represent ideal tumor-specific target antigens for immunotherapy of cervical cancer. Recently, the recognition of dendritic cells (DC) as powerful antigen-presenting cells, capable of inducing primary T-cell responses in vitro and in vivo, has generated widespread interest in DC-based immunotherapy of several human malignancies. This review summarizes the therapeutic clinical trials and the different preclinical research strategies that are under investigation, with a particular emphasis on the use of autologous DC-pulsed HPV16 or 18 E7 oncoproteins as therapeutic vaccines against cervical cancer.
A conserved MADS-box phosphorylation motif regulates differentiation and mitochondrial function in skeletal, cardiac, and smooth muscle cells (2015)

Exposure to metabolic disease during fetal development alters cellular differentiation and perturbs metabolic homeostasis, but the underlying molecular regulators of this phenomenon in muscle cells are not completely understood. To address this, we undertook a computational approach to identify cooperating partners of the myocyte enhancer factor-2 (MEF2) family of transcription factors, known regulators of muscle differentiation and metabolic function. We demonstrate that MEF2 and the serum response factor (SRF) collaboratively regulate the expression of numerous muscle-specific genes, including microRNA-133a (miR-133a). Using tandem mass spectrometry techniques, we identify a conserved phosphorylation motif within the MEF2 and SRF Mcm1 Agamous Deficiens SRF (MADS)-box that regulates miR-133a expression and mitochondrial function in response to a lipotoxic signal. Furthermore, reconstitution of MEF2 function by expression of a neutralizing mutation in this identified phosphorylation motif restores miR-133a expression and mitochondrial membrane potential during lipotoxicity. Mechanistically, we demonstrate that miR-133a regulates mitochondrial function through translational inhibition of a mitophagy and cell death modulating protein, called Nix. Finally, we show that rodents exposed to gestational diabetes during fetal development display muscle diacylglycerol accumulation, concurrent with insulin resistance, reduced miR-133a, and elevated Nix expression, as young adult rats. Given the diverse roles of miR-133a and Nix in regulating mitochondrial function, and proliferation in certain cancers, dysregulation of this genetic pathway may have broad implications involving insulin resistance, cardiovascular disease, and cancer biology.
Protonic Conduction and O-H Bonding State in Phosphate Glasses (1990)

Abstract Protons in alkaline-earth phosphate glasses give much higher mobility than those in silicate glasses. This is caused by the difference in O-H bonding state. Protons in P-OH form a strong hydrogen bonding with oxygen anion, resulting in formation of weak O-H bond. The electrical conductivity of the glasses is proportional to the square of the proton concentration. The activation energy for the conduction is related to νOH, peak wavenumber of infrared absorption band (around∼3000 cm−1), due to fundamental O-H stretching vibration. The mobility of protons increases with decreasing νOH, which depends on the species of ions included in the phosphate glasses.
Distinct patterns of E-cadherin CpG island methylation in papillary, follicular, Hurthle's cell, and poorly differentiated human thyroid carcinoma. (1998)

Expression of the invasion/metastasis suppressor, E-cadherin, is diminished or lost in thyroid carcinomas. Yet, mutational inactivation of E-cadherin is rare. Herein, we show that this loss is associated with hypermethylation of the E-cadherin 5' CpG island in a panel of human thyroid cancer cell lines. This aberrant methylation is evident in 83% of papillary thyroid carcinoma, 11% of follicular thyroid carcinoma, 40% of Hurthle's cell carcinoma, and 21% of poorly differentiated thyroid carcinomas. Contrary to previous reports, the majority of these poorly differentiated thyroid carcinomas express E-cadherin, but often within the cytoplasm rather than at the cell surface. Together, our data indicate that the invasion/metastasis suppressor function of E-cadherin is frequently compromised in human papillary, Hurthle's cell, and poorly differentiated thyroid carcinoma by epigenetic and biochemical events.
Clinical experience with the TandemHeart percutaneous ventricular assist device as a bridge to cardiac transplantation. (2008)

Cardiac support with a ventricular assist device is among the few treatments for heart-failure patients who have profound cardiogenic shock unresponsive to vasopressors and intra-aortic balloon pumps. The TandemHeart percutaneous ventricular assist device can provide temporary support until another device can be placed or a donor heart becomes available. We examined the TandemHeart's effect on cardiac index, central venous pressure, mixed venous oxygen saturation, creatinine, mean arterial pressure, urine output, and 30-day mortality rate in 5 heart-failure patients (2 with nonischemic and 3 with ischemic cardiomyopathy; mean preoperative left ventricular ejection fraction, 0.17 +/- 0.056). Two patients were undergoing cardiopulmonary resuscitation when the device was inserted. The average duration of TandemHeart support was 7.6 +/- 3.2 days; all patients were successfully bridged to transplantation. The TandemHeart improved the cardiac index (1.9 +/- 0.3 vs 3.5 +/- 0.8 L/[min.m2], P= 0.01), mean arterial pressure (69 +/- 12.5 vs 91 +/- 4.3 mmHg, P=0.009), mixed venous oxygen saturation (45.4 +/- 14.3 vs 71.4 +/- 7.5, P=0.009), and urine output (1,861 +/- 988 vs 4,314 +/- 1,346 mL/hr, P=0.01). The device decreased central venous pressure (21.2 +/- 7.4 vs 12.8 +/- 5.9 mmHg, P=0.02) and pressor requirements (2.4 +/- 1.1 vs 1.0 +/- 0.7 agents, P=0.02). Average long-term follow-up after heart transplantation was 8.4 +/- 9.9 months, with no deaths. We conclude that the TandemHeart can provide hemodynamic support for patients with profound, refractory cardiogenic shock. Furthermore, the device can bridge patients to cardiac transplantation and can be placed percutaneously, without invasive surgery.
Preparation of Cr-Doped V2O3 Films by Sol-Gel Processing and Their Resistivity-Temperature Characteristics. (1999)

Films of Cr-added V2O3 were prepared by reduction of sol-gel-derived V2O5 films at temperatures over 500°C in flowing H2. Auger electron spectroscopy of the films showed that atoms of V and O were distributed homogeneously on thickness direction. From X-ray photoelectron spectroscopy of the films, it was found that the reduction was enhanced with an increase of the reduction temperature, however, even at 900°C reduction valence of V still interposed between +3 and +4. The specific resistivity (ρ)-temperature (T) curves of the films showed antiferromagnetic insulator (AFI)-metal (M) transitions, but not metal (M)-insulator (I) transitions. Grain diameters of Cr-doped V2O3 powders prepared in the same way of the films increased by sintering at 1000 to 1350°C for 5h in flowing mixture gas (95vol%N2+5vol%H2). The M-I transitions was observed in the ρ-T curves of these powders heated at 1200°C and higher. Absence of M-I transition of sol-gel-derived V2O3 films was considered to be due to small grain sizes and/or small crystallite size of films.
Neurogenic peripheral blood‐derived adult stem cells (2006)

Self-renewing neural stem cells are routinely isolated from embryonic or fetal sources. We describe here the development of several clonal lines of neurogenic self-renewing multipotent progenitor cells that have been isolated from the blood of adult green fluorescent protein transgenic swine. The cells are designated “peripheral blood derived multipotent adult progenitor cells” (PBD-MAPCs). These cells grow as spheroids (reminiscent of neurospheres) and readily proliferate under conditions chosen to promote self-renewal without differentiation. We have isolated 5 independent lines of these cells from 2 different animals. When plated under neurogenic differentiation conditions PBD-MAPCs rapidly differentiate into cells that express several neural markers including microtubule-associated protein 2ab, myelin basic protein, NeuN, glial fibrillary acidic protein, and tyrosine hydroxylase. The expression of these markers was confirmed by both confocal immunofluorescence and immunoblots. These cells exhibit the diverse morphologies that are consistent with neurons and glia. Furthermore, when grown on a thin film of MatrigelTM the cells exhibit robust migration and process/projection formations and appear to engage in dynamic cell-cell interrogations and interactions. We conclude that PBD-MAPCs represent adult neurogenic stem cells that can be used as cell culture models of neural development and as reagents for stem cell therapy for many neurological disorders. Supported by NIH PO1HL52490.
Modulation of Intracerebral Glycosidase Activities by Intraperitoneal Administration of Glycosidase Inhibitors. (1991)

When administered to mice, enzyme inhibitors exert extensive effects that cannot be expected from their direct actions. On the basis of our previous observations [1], we tested how glycosidase inhibitors influence the intracerebral activities of various hydrolytic enzymes including glycosidases. The results showed that, when administered intraperitoneally, mannostatin and nagstatin stimulated, while siastatin and pyridindolol suppressed, intracerebral activities of glycosidases. The effects showed dose dependency. Although the above inhibitors influenced various other enzymatic activities as well, no particular significance could be attached to such effects because of the absence of dose dependency.
Counting People by Estimating People Flows (2020)

Modern methods for counting people in crowded scenes rely on deep networks to estimate people densities in individual images. As such, only very few take advantage of temporal consistency in video sequences, and those that do only impose weak smoothness constraints across consecutive frames. In this paper, we advocate estimating people flows across image locations between consecutive images and inferring the people densities from these flows instead of directly regressing them. This enables us to impose much stronger constraints encoding the conservation of the number of people. As a result, it significantly boosts performance without requiring a more complex architecture. Furthermore, it allows us to exploit the correlation between people flow and optical flow to further improve the results. We also show that leveraging people conservation constraints in both a spatial and temporal manner makes it possible to train a deep crowd counting model in an active learning setting with much fewer annotations. This significantly reduces the annotation cost while still leading to similar performance to the full supervision case.
Ferroelectric Nonvolatile Memory Technology and Its Applications (1996)

Nonvolatile memory utilizing ferroelectric material is expected to be the ultimate memory due to its theoretical low power operation and fast access. We integrated a ferroelectric thin film using a standard complementary metal-oxide-semiconductor (CMOS) process and evaluated its basic characteristics and reliability including endurance and imprint effect. The film was prepared using a spin-on sol-gel method. A ferroelectric thin film formed using liquid source misted chemical deposition (LSMCD) was found to have almost the same characteristics as those of the film formed by the sol-gel method. No effects of the ferroelectric process on the CMOS transistors were observed. Design of ferroelectric memory cells and applications of the ferroelectric nonvolatile memory have been reviewed.
Low sidelobe compact reflector antenna using backfire primary radiator for Ku-band mobile satellite communication system on board vessel (2013)

We developed low side lobe compact reflector antennas of aperture diameter of 60cm and 120cm. They are corresponding to 30 and 60 wavelength. These antennas are axial symmetry antennas using a backfire primary radiator. They are used in Ku-band mobile satellite communication. The requirements of antennas are low side lobes and low cross polarization. The antennas have satisfied ITU-R recommendation S.580-6. This paper introduces the structures and the radiation patterns of the antennas.
In Reply: (2018)

Ke, Yuyong PhD; Simard, Jean-Nicolas MSc; Gonthier, Renaud BSc; Martel, Céline PhD; Vaillancourt, Mario BSc; Portman, David MD; Bélanger, Alain PhD; Labrie, Fernand MD, PhD Author Information