gebhart/patrepeval huggingface dataset:
Structure, Sulfatide Binding Properties, and Inhibition of Platelet Aggregation by a Disabled-2 Protein-derived Peptide (2012)

Disabled-2 (Dab2) targets membranes and triggers a wide range of biological events, including endocytosis and platelet aggregation. Dab2, through its phosphotyrosine-binding (PTB) domain, inhibits platelet aggregation by competing with fibrinogen for α(IIb)β(3) integrin receptor binding. We have recently shown that the N-terminal region, including the PTB domain (N-PTB), drives Dab2 to the platelet membrane surface by binding to sulfatides through two sulfatide-binding motifs, modulating the extent of platelet aggregation. The three-dimensional structure of a Dab2-derived peptide encompassing the sulfatide-binding motifs has been determined in dodecylphosphocholine micelles using NMR spectroscopy. Dab2 sulfatide-binding motif contains two helices when embedded in micelles, reversibly binds to sulfatides with moderate affinity, lies parallel to the micelle surface, and when added to a platelet mixture, reduces the number and size of sulfatide-induced aggregates. Overall, our findings identify and structurally characterize a minimal region in Dab2 that modulates platelet homotypic interactions, all of which provide the foundation for rational design of a new generation of anti-aggregatory low-molecular mass molecules for therapeutic purposes.
Treatment of Alveolar Hypoventilation in a Six-Year-Old Girl with Intermittent Positive Pressure Ventilation Through a Nose Mask (1987)

Persons with alveolar hypoventilation have abnormal daytime arterial blood gases and abnormal responses to hypercapnia and hypoxia in the absence of any identifiable lung or neuromuscular disease. The underlying defect in the control of breathing has not, however, been confirmed. We studied a 6-yr-old girl who was admitted in respiratory failure after a long history of disturbed breathing awake and asleep, which had been diagnosed as primary alveolar hypoventilation, (PaCO2 = 120). After several days of endotracheal intubation and assisted ventilation, her condition improved and she was extubated. At this time her ventilatory response to hypoxia was absent (VE/SaO2:0.1 l/min/% at a CO2 of 45) and there was a right-shifted response to hypercapnia (VE/PaCO2:2.6 l/min/mmHg). As obstructive sleep apnea was suspected, nocturnal nasal continuous positive airway pressure (CPAP) was tried; however, it was not effective in maintaining arterial oxyhemoglobin saturation. Definite central apneas were observed during sleep both with and without nasal CPAP, and there was an absence of snoring. Her condition deteriorated, and there was a progressive increase in her awake arterial CO2 levels for a period of 4 wk. The IPPV with 5 cm H2O of PEEP was administered through a nose mask during sleep and this maintained both oxygen saturation and transcutaneous CO2 levels within the normal range. After 10 days of nocturnal assisted ventilation, the hypercapnic response returned to the normal position (VE/CO2:2.1 l/min/mmHg).(ABSTRACT TRUNCATED AT 250 WORDS)
Real-Time Label-Free Targeting Assessment and in Vitro Characterization of Curcumin-Loaded Poly-lactic-co-glycolic Acid Nanoparticles for Oral Colon Targeting (2019)

The exploitation of curcumin for oral disease treatment is limited by its low solubility, poor bioavailability, and low stability. Surface-functionalized poly-lactic-<i>co</i>-glycolic acid (PLGA) nanoparticles (NPs) have shown promising results to ameliorate selective delivery of drugs to the gastro-intestinal tract. In this study, curcumin-loaded PLGA NPs (C-PLGA NPs) of about 200 nm were surface-coated with chitosan (CS) for gastro-intestinal mucosa adhesion, wheat germ agglutinin (WGA) for colon targeting or GE11 peptide for tumor colon targeting. Spectrometric and zeta potential analyses confirmed the successful functionalization of the C-PLGA NPs. Real-time label-free assessment of the cell membrane-NP interactions and NP cell uptake were performed by quartz crystal microbalance coupled with supported lipid bilayers and by surface plasmon resonance coupled with living cells. The study showed that CS-coated C-PLGA NPs interact with cells by the electrostatic mechanism, while both WGA- and GE11-coated C-PLGA NPs interact and are taken up by cells by specific active mechanisms. In vitro cell uptake studies corroborated the real-time label-free assessment by yielding a curcumin cell uptake of 7.3 ± 0.3, 13.5 ± 1.0, 27.3 ± 4.9, and 26.0 ± 1.3 μg per 10<sup>4</sup> HT-29 cells for noncoated, CS-, WGA-, and GE11-coated C-PLGA NPs, respectively. Finally, preliminary in vivo studies showed that the WGA-coated C-PLGA NPs efficiently accumulate in the colon after oral administration to healthy Balb/c mice. In summary, the WGA- and GE11-coated C-PLGA NPs displayed high potential for application as active targeted carriers for anticancer drug delivery to the colon.
Stability Demands for Laser-systems in Plasma Acceleration (2019)

We report on the long-term stability of the 200TW ANGUS laser system and approaches for overcoming the limits of Ti:Sapphire laser systems to fulfill the stability demands of laser-plasma accelerators.
Supplementary Movie 6 from Tivantinib (ARQ 197) Exhibits Antitumor Activity by Directly Interacting with Tubulin and Overcomes ABC Transporter–Mediated Drug Resistance (2023)

&lt;p&gt;Vincristine treated (SK-MEL-28 Fucci).&lt;/p&gt;
Organic Ring-Opening Polymerization Catalysts: Reactivity Control by Balancing Acidity (2018)

Organocatalysts derived from thioureas and amines exhibit high functional group tolerance and extraordinary selectivities for ring-opening relative to chain transesterification. The modest activities of the thiourea/amine catalysts prompted a detailed investigation of ureas and thiourea with organic bases for the ring-opening polymerization of lactones. An array of ureas or thioureas and organic bases were evaluated to assess the effect of the acidity of the urea (thiourea) and the basicity of the base cocatalyst on the activity for ring-opening polymerization. These studies reveal that for a given urea or thiourea stronger bases lead to faster rates. For a given base, the observed catalytic activity is highest when the acidity of the (thio)urea is closely matched with that of the B–H+. For ureas and thioureas of comparable acidity, the urea/base catalyst systems are considerably more active than the corresponding thiourea/base systems. These results are consistent with two mechanisms: one mediated by deprotonated (thio)urea anions when (thio)ureas are combined with bases of sufficient basicity and one mediated by neutral (thio)ureas when the base is incapable of deprotonating the (thio)urea. Opposing trends in reactivity for (thio)urea anions and neutral (thio)ureas as a function of (thio)urea acidity lead to the maximal activity when the acidities of the (thio)ureas are closely matched with that of the protonated base (B–H+). These findings provide the basis for understanding the reactivity of ring-opening polymerization cocatalysts as well as guidelines for the rational design of other acid/base catalyst pairs.
Treatment Planning System for Electron FLASH Radiotherapy: Open-source for Clinical Implementation (2021)

Purpose: A Monte Carlo (MC) beam model and its implementation in a clinical treatment planning system (TPS, Varian Eclipse) are presented for a modified ultra-high dose-rate electron FLASH radiotherapy (eFLASH-RT) LINAC. Methods: The gantry head without scattering foils or targets, representative of the LINAC modifications, was modelled in Geant4. The energy spectrum (σE) and beam source emittance cone angle (θcone) were varied to match the calculated and Gafchromic film measured central-axis percent depth dose (PDD) and lateral profiles. Its Eclipse configuration was validated with measured profiles of the open field and nominal fields for clinical applicators. eFLASH-RT plans were MC forward calculated in Geant4 for a mouse brain treatment and compared to a conventional (Conv-RT) plan in Eclipse for a human patient with metastatic renal cell carcinoma. Results: The beam model and its Eclipse configuration agreed best with measurements at σE=0.5 MeV and θcone=3.9+/-0.2 degrees to clinically acceptable accuracy (the absolute average error was within 1.5% for in-water lateral, 3% for in-air lateral, and 2% for PDD). The forward dose calculation showed dose was delivered to the entire mouse brain with adequate conformality. The human patient case demonstrated the planning capability with routine accessories in relatively complex geometry to achieve an acceptable plan (90% of the tumor volume receiving 95% and 90% of the prescribed dose for eFLASH and Conv-RT, respectively). Conclusion: To the best of our knowledge, this is the first functional beam model commissioned in a clinical TPS for eFLASH-RT, enabling planning and evaluation with minimal deviation from Conv-RT workflow. It facilitates the clinical translation as eFLASH-RT and Conv-RT plan quality were comparable for a human patient. The methods can be expanded to model other eFLASH irradiators.
Modeling Multimodal Clues in a Hybrid Deep Learning Framework for Video Classification (2018)

Videos are inherently multimodal. This paper studies the problem of exploiting the abundant multimodal clues for improved video classification performance. We introduce a novel hybrid deep learning framework that integrates useful clues from multiple modalities, including static spatial appearance information, motion patterns within a short time window, audio information, as well as long-range temporal dynamics. More specifically, we utilize three Convolutional Neural Networks (CNNs) operating on appearance, motion, and audio signals to extract their corresponding features. We then employ a feature fusion network to derive a unified representation with an aim to capture the relationships among features. Furthermore, to exploit the long-range temporal dynamics in videos, we apply two long short-term memory (LSTM) networks with extracted appearance and motion features as inputs. Finally, we also propose refining the prediction scores by leveraging contextual relationships among video semantics. The hybrid deep learning framework is able to exploit a comprehensive set of multimodal features for video classification. Through an extensive set of experiments, we demonstrate that: 1) LSTM networks that model sequences in an explicitly recurrent manner are highly complementary to the CNN models; 2) the feature fusion network that produces a fused representation through modeling feature relationships outperforms a large set of alternative fusion strategies; and 3) the semantic context of video classes can help further refine the predictions for improved performance. Experimental results on two challenging benchmarks-the UCF-101 and the Columbia Consumer Videos (CCV)-provide strong quantitative evidence that our framework can produce promising results: 93.1% on the UCF-101 and 84.5% on the CCV, outperforming several competing methods with clear margins.
High-Temperature (250 °C / 500 °F) 19 000 min $^{\hbox{-}1}$ BLDC Fan for Forced Air-Cooling of Advanced Automotive Power Electronics (2014)

In an increasing number of application areas and industry sectors, such as the automotive, aerospace, military, or oil and gas industry, a trend towards higher ambient temperature rating from 120 °C upward for electrical machines and power electronic converters can be observed. Forced air-cooling of power electronic converters offers reduced complexity of the cooling circuit compared to water-cooling. For air-cooled, high-ambient temperature rated converters, fans are required to withstand these temperatures and still feature performance comparable to standard conditions in order to still enable a high converter power density and efficiency. Commercially available fans for power electronics cooling are typically rated up to 75°C, rarely fans are specified up to 105°C. In this paper, the electrical and mechanical design of a 40 mm × 40 mm × 28 mm fan is presented in detail that offers an operational temperature range from -40 °C to 250°C at the rated speed of 19 000 min <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-1</sup> and comparable fluid dynamic performance in terms of static pressure and volume flow at 120 °C as commercial high performance fans at 20°C. The three-phase brushless direct current machine driving the fan is integrated into its hub and has got an input power of 15 W. The fan can be driven using a three-phase inverter supplied from 12 V dc voltage with an inverter switching frequency of less than 1.3 kHz.
Supplementary Figure Legend from Epithelial-to-Mesenchymal Transition and Autophagy Induction in Breast Carcinoma Promote Escape from T-cell–Mediated Lysis (2023)

&lt;p&gt;PDF file - 108K&lt;/p&gt;
Age and Giardia intestinalis Infection Impact Canine Gut Microbiota (2021)

<i>Giardia intestinalis</i> is a flagellated protozoan responsible for giardiosis (also called giardiasis in humans), the most prevalent and widespread parasitic infection in humans and mammals worldwide. The intestinal microbiota is highly diverse and any alteration in its composition may impact on the health of the host. While studies on the mouse model of giardiosis described the role of the gut microbiota in host susceptibility to infection by the parasite, little is known about the gut microbiota during natural infections in dogs and particularly in puppies. In this study, we monitored naturally <i>G. intestinalis</i>-infected puppies for 3 months and quantified cyst excretion every 2 weeks. All puppies remained subclinically infected during the sampling period as confirmed by fecal examination. In parallel, we performed 16S Illumina sequencing of fecal samples from the different time points to assess the impact of <i>G. intestinalis</i> infection on gut microbiota development of the puppies, as well as gut health markers of immunity such as fecal IgA and calprotectin. Sequencing results revealed that the canine fecal microbiota of <i>Giardia</i>-infected puppies becomes more complex and less diverse with increasing age. In addition, significant differences in the structure of the microbiota were observed between puppies with high and low <i>Giardia</i> cyst excretion. Chronic subclinical <i>G. intestinalis</i> infection appears to be associated with some detrimental structural changes in the gut microbiota. <i>G. intestinalis</i>-associated dysbiosis is characterized by an enrichment of facultative anaerobic, mucus-degrading, pro-inflammatory species and opportunistic pathogens, as well as a reduction of <i>Lactobacillus johnsonii</i> at specific time points. Calprotectin levels increased with age, suggesting the establishment of chronic low-grade inflammation in puppies. Further work is needed to demonstrate whether these alterations in the canine gut microbiota could lead to a dysbiosis-related disease, such as irritable bowel syndrome (IBS) or inflammatory bowel disease (IBD).
Digital Cognitive Aids to Support Adaptation of Surgical Processes to COVID-19 Protective Policies (2020)

Surgical processes are rapidly being adapted to address the COVID-19 pandemic, with changes in procedures and responsibilities being made to protect both patients and medical teams. These process changes put new cognitive demands on the medical team and increase the likelihood of miscommunication, lapses in judgment, and medical errors. We describe two process model driven cognitive aids, referred to as the Narrative View and the Smart Checklist View, generated automatically from models of the processes. The immediate perceived utility of these cognitive aids is to support medical simulations, particularly when frequent adaptations are needed to quickly respond to changing operating room guidelines.
Strategies for loading dendritic cells with hepatitis C NS5a antigen and inducing protective immunity (2008)

Dendritic cell (DC)-based vaccination strategies are promising for the treatment of cancers and infectious diseases including hepatitis C virus (HCV). As the induction of T cell-mediated immune responses by DC vaccination is highly dependent on efficient antigen loading of the DCs, the purpose of this study was to identify an optimal nonviral DC loading strategy for HCV NS5a. Furthermore, the efficacy of immunization with the NS5a-loaded DCs in comparison to plasmid encoding NS5a and NS5a protein was evaluated. Transfection of DCs with mRNA was most efficient with close to 100% of DCs expressing NS5a, whereas approximately 10% of protein-pulsed DCs and <1% of plasmid-transfected DCs expressed NS5a, suggesting remarkably different loading efficiencies. Vaccination of mice with NS5a mRNA-transfected DCs or NS5a protein-pulsed DCs resulted in significantly stronger CD4(+) and CD8(+) T-cell responses and protection from challenge with vaccinia virus expressing NS3/NS4/NS5, in comparison to vaccination with NS5a DNA-transfected DCs, plasmid encoding NS5 or rNS5a protein formulated with alum. Furthermore, vaccination with NS5a mRNA-transfected DCs was superior to vaccination with rNS5a-pulsed DCs. These data have important clinical implications, with mRNA-transfected DCs providing a safe and effective vaccination strategy against hepatitis C and possibly other pathogens.
X-ray diagnostics for electron cyclotron resonance processing plasmas (1992)

Electron cyclotron resonance processing plasmas have been shown to produce low-energy x rays. X radiation was detected in nitrogen plasmas from energies of 1 to 17 keV for microwave input powers of 800 and 1000 W. The x-ray flux decreased with increasing pressure over the range of 0.5 to 3.5 mTorr. Temperatures corresponding to the component of the electron velocity distributions responsible for creating the x rays were estimated from the slopes of the tails of the x-ray spectra and also decreased with increasing pressure. The x-ray flux measured by the detector could be decreased substantially by changing the magnetic field configuration. In general, it was found that measurable x radiation was produced whenever the surface described by the value of magnetic field which corresponds to that for cyclotron resonance intersects the vacuum chamber walls and/or other solid surfaces inside the chamber.
“One-Pot” Synthesis of a Thermally Stable Blue Emitter: Poly[spiro(fluorene-9,9′-(2′-phenoxy-xanthene)] (2008)

ADVERTISEMENT RETURN TO ISSUEPREVCommunication to the...Communication to the EditorNEXT"One-Pot" Synthesis of a Thermally Stable Blue Emitter: Poly[spiro(fluorene-9,9′-(2′-phenoxy-xanthene)]Shaune L. McFarlane, Leah S. Coumont, Davin G. Piercey, Robert McDonald, and Jonathan G. C. Veinot*View Author Information Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2G2* Corresponding author. E-mail: [email protected]Cite this: Macromolecules 2008, 41, 21, 7780–7782Publication Date (Web):October 10, 2008Publication History Received14 January 2008Revised25 August 2008Published online10 October 2008Published inissue 11 November 2008https://pubs.acs.org/doi/10.1021/ma801123dhttps://doi.org/10.1021/ma801123drapid-communicationACS PublicationsCopyright © 2008 American Chemical SocietyRequest reuse permissionsArticle Views1123Altmetric-Citations25LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information SUBJECTS:Aromatic compounds,Materials,Oxidative stability,Polymers,Stability Get e-Alerts
Hemoglobin–Albumin Cluster Incorporating a Pt Nanoparticle: Artificial O2 Carrier with Antioxidant Activities (2014)

A covalent core-shell structured protein cluster composed of hemoglobin (Hb) at the center and human serum albumins (HSA) at the periphery, Hb-HSAm, is an artificial O2 carrier that can function as a red blood cell substitute. Here we described the preparation of a novel Hb-HSA3 cluster with antioxidant activities and its O2 complex stable in aqueous H2O2 solution. We used an approach of incorporating a Pt nanoparticle (PtNP) into the exterior HSA unit of the cluster. A citrate reduced PtNP (1.8 nm diameter) was bound tightly within the cleft of free HSA with a binding constant (K) of 1.1×10(7) M(-1), generating a stable HSA-PtNP complex. This platinated protein showed high catalytic activities for dismutations of superoxide radical anions (O2•-) and hydrogen peroxide (H2O2), i.e., superoxide dismutase and catalase activities. Also, Hb-HSA3 captured PtNP into the external albumin unit (K = 1.1×10(7) M(-1)), yielding an Hb-HSA3(PtNP) cluster. The association of PtNP caused no alteration of the protein surface net charge and O2 binding affinity. The peripheral HSA-PtNP shell prevents oxidation of the core Hb, which enables the formation of an extremely stable O2 complex, even in H2O2 solution.
Privacy Preserving Burst Detection of Distributed Time Series Data Using Linear Transforms (2007)

In this paper, we consider burst detection within the context of privacy. In our scenario, multiple parties want to detect a burst in aggregated time series data, but none of the parties want to disclose their individual data. Our approach calculates bursts directly from linear transform coefficients using a cumulative sum calculation. In order to reduce the chance of a privacy breech, we present multiple data perturbation strategies and compare the varying degrees of privacy preserved. Our strategies do not share raw time series data and still detect significant bursts. We empirically demonstrate this using both real and synthetic distributed data sets. When evaluating both privacy guarantees and burst detection accuracy, we find that our percentage thresholding heuristic maintains a high degree of privacy while accurately identifying bursts of varying widths
Enhanced interband-resonant light modulation by intersubband-resonant light in selectively n-doped quantum wells (1995)

The interband-resonant light modulation by the intersubband-resonant light in selectively n-doped quantum wells is investigated. The modulation efficiency depends greatly on the degree of nonlinear optical coupling between the interband and intersubband-resonant lights. It is shown theoretically and experimentally that the selective n-doping in the barrier layers of the quantum wells is very effective to increase the nonlinear coupling degree and thus the modulation efficiency. The thermal and the hot carrier effects on the modulation are also discussed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
ChemInform Abstract: Gold(I)‐Catalyzed Synthesis of Optically Active 1,4‐Oxazepan‐7‐ones. (2015)

Abstract A novel one‐step synthesis of enantioenriched 1,4‐oxazepan‐7‐ones is achieved by cyclization of optically active alkynyl esters in the presence of Ph 3 PAuCl and Cu(OTf) 2 as the catalysts.