gebhart/patrepeval huggingface dataset:
A Unique Region in Bacteriophage T7 DNA Polymerase Important for Exonucleolytic Hydrolysis of DNA (2004)

A crystal structure of the bacteriophage T7 gene 5 protein/Escherichia coli thioredoxin complex reveals a region in the exonuclease domain (residues 144-157) that is not present in other members of the E. coli DNA polymerase I family. To examine the role of this region, a genetically altered enzyme that lacked residues 144-157 (T7 polymerase (pol) Delta144-157) was purified and characterized biochemically. The polymerase activity and processivity of T7 pol Delta144-157 on primed M13 DNA are similar to that of wild-type T7 DNA polymerase implying that these residues are not important for DNA synthesis. The ability of T7 pol Delta144-157 to catalyze the hydrolysis of a phosphodiester bond, as judged from the rate of hydrolysis of a p-nitrophenyl ester of thymidine monophosphate, also remains unaffected. However, the 3'-5' exonuclease activity on polynucleotide substrates is drastically reduced; exonuclease activity on single-stranded DNA is 10-fold lower and that on double-stranded DNA is 20-fold lower as compared with wild-type T7 DNA polymerase. Taken together, our results suggest that residues 144-157 of gene 5 protein, although not crucial for polymerase activity, are important for DNA binding during hydrolysis of polynucleotides.
Evidence from hydrogen-1 and carbon-13 nuclear magnetic resonance studies that the dissociation rate of oxytocin from bovine neurophysin at neutral pH is slow (1978)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEvidence from hydrogen-1 and carbon-13 nuclear magnetic resonance studies that the dissociation rate of oxytocin from bovine neurophysin at neutral pH is slowMichael Blumenstein, Victor J. Hruby, and Diane M. YamamotoCite this: Biochemistry 1978, 17, 23, 4971–4977Publication Date (Print):November 14, 1978Publication History Published online1 May 2002Published inissue 14 November 1978https://pubs.acs.org/doi/10.1021/bi00616a018https://doi.org/10.1021/bi00616a018research-articleACS PublicationsRequest reuse permissionsArticle Views34Altmetric-Citations12LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A phase I study of an agonist anti-CD27 human antibody (CDX-1127) in patients with advanced hematologic malignancies or solid tumors (2013)

Meeting abstracts CD27, a member of the tumor necrosis factor receptor superfamily, is constitutively expressed on the majority of mature T cells, memory B cells, and a portion of NK cells. Previously we have reported anti-tumor and immunological properties of the fully human anti-CD27 mAb 1F5 (CDX
Z pinch driven inertial confinement fusion target physics research at Sandia National Laboratories (1999)

Three hohlraum concepts are being pursued at Sandia National Laboratories (SNL) to investigate the possibility of using pulsed power driven magnetic implosions (Z pinches) to drive targets capable of fusion yields in the range 200-1000 MJ. This research is being conducted on SNL's Z facility, which is capable of driving peak currents of 20 MA in various Z pinch load configurations that produce implosion velocities as high as 7.5 × 107cm/s, X ray energies of 1-2 MJ and X ray powers of 100-250 TW. The first concept, denoted dynamic hohlraum, has achieved a temperature of 180 ± 14 eV in a configuration suitable for driving capsules. In addition, this concept has also achieved a temperature of 230 ± 18 eV in an arrangement suitable for driving an external hohlraum. The second concept, denoted static walled hohlraum, has achieved temperatures of ∼80-100 eV. Experimental investigation of the third concept, denoted Z pinch driven hohlraum, has recently begun. The article discusses each of these hohlraum concepts and provides an overview of the experiments that have been conducted on these systems to date.
ChemInform Abstract: 2‐Benzopyrylium Salts. Part 39. Recyclization Reactions of 2‐Benzopyrylium Salts During the Treatment with Ethyl Vinyl Ether and Active Methylene Compounds. (1990)

Abstract The benzopyrylium salts (I) are coupled with ethyl vinyl ether (II), acetoacetic ester (IV), or malononitrile (VII) to give the naphthalene derivatives (III), (V), (IX), (X), and (XIII).
Superhydrophobic or Hydrophilic Porous Metallic/Ceramic Tubular Membranes for Continuous Separations of Biodiesel–Water W/O and O/W Emulsions (2019)

This paper reports the effective surface effect of porous inorganic membranes on improving the emulsion separations (i.e., perm-selective extraction of either oil phase or aqueous phase from the emulsions). A novel tubular form of superwetting, porous, stainless steel/ceramic membranes were demonstrated for enabling continuous separations of oil–water emulsions, extracting either oil phase or aqueous phase from the flowing emulsions. The superhydrophobic membranes consist of macroporous (4 μm) stainless-steel SS434 tube with inner wall surface modified with superhydrophobicity (>150° contact angle) via perfluoro-silane grafting functionalization. The (super)hydrophilic membranes consist of 4-μm porous stainless-steel SS434 tube without/with inner wall coated with nanoporous alumina (6 nm average pore size), the surface of which was further modified with superhydrophilicity via Hydrophil-S solution deposition. With the cross-flow membrane filtration setup, the surface-engineered tubular membranes were studied for perm-selective extraction of aqueous or oil phase from oil–water emulsions (water-in-oil W/O and oil-in-water O/W) that are relevant to a real industrial biodiesel (FAME) production process. The superhydrophobic membrane (4 μm pore size) has demonstrated capability of extracting nearly 100% pure oil while rejecting water phase when either W/O or O/W emulsions were tested as feed. The superhydrophobic membrane showed distinct advantage of 2-orders-of-magnitude-higher flux extraction of oil at 100 times higher “break-through” pressures. On the other hand, the super/hydrophilic nanoporous membranes (6 nm) have shown selective water permeation (separation factor up to 35) when biodiesel-relevant W/O emulsions were tested. Finally, the membrane function is discussed from the perspective of improving industrial biodiesel processing yield by overcoming equilibrium limitations during the biodiesel formation reactions. Future work on in situ reaction–separation experiments are envisioned.
Algorithms for simultaneous motion control of multiple T. pyriformis cells: Model predictive control and Particle Swarm Optimization (2015)

This paper investigates the use of single control signal (magnetic field direction) and PSO-MPC algorithm to control multiple magnetized Tetrahymena pyriformis (T. pyriformis) cells to move from their initial positions to their target positions simultaneously while avoiding the obstacle. The magnetized T. pyriformis cells are generated by adding iron-oxide spherical particles into the cells. We control the cells' moving direction by changing the magnetic field direction. Based on Model Predictive Control (MPC) algorithm, we define a cost function which is composed of the target cost function and the obstacle potential function. The target cost function is to measure the sum of differences between cells' predicted positions and their target positions. The obstacle potential function is used to measure the repulsive force of the obstacle. The input variables of the cost function are the sequence of control signals. We use Particle Swarm Optimization (PSO) method to find a cost value which is close to the global minimum of the cost function. In the experimental result section, we show the control of three m3pi robots to move from their initial positions to their target positions with avoiding the obstacle. Since the similar control strategy has successfully controlled one T. pyriformis cell in our previous work, we believe our PSO-MPC algorithm is applicable on the multiple T. pyriformis cells' control task.
A new time-independent approach to the study of atom-diatom reactive collisions: theory and application (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA new time-independent approach to the study of atom-diatom reactive collisions: theory and applicationDaniel. Neuhauser and Michael. BaerCite this: J. Phys. Chem. 1990, 94, 1, 185–189Publication Date (Print):January 1, 1990Publication History Published online1 May 2002Published inissue 1 January 1990https://doi.org/10.1021/j100364a029RIGHTS & PERMISSIONSArticle Views83Altmetric-Citations39LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (1 MB) Get e-Alerts
Delivery of definable number of drug or growth factor loaded poly(dl-lactic acid-co-glycolic acid) microparticles within human embryonic stem cell derived aggregates (2013)

Embryoid bodies (EBs) generated from embryonic stem cells are used to study processes of differentiation within a three dimensional (3D) cell environment. In many instances however, EBs are dispersed to single cell suspensions with a subsequent monolayer culture. Moreover, where the 3D integrity of an EB is maintained, cytokines or drugs of interest to stimulate differentiation are often added directly to the culture medium at fixed concentrations and effects are usually limited to the outer layers of the EB. The aim of this study was to create an EB model with localised drug and or growth factor delivery directly within the EB. Using poly(DL-lactic acid-co-glycolic acid) microparticles (MPs) with an average diameter of 13μm, we have demonstrated controllable incorporation of defined numbers of MPs within human ES cell derived EBs, down to 1 MP per EB. This was achieved by coating MPs with human ES cell lysate and centrifugation of specific ratios of ES cells and MPs to form 3D aggregates. Using MPs loaded with simvastatin (pro or active drug) or BMP-2, we have demonstrated osteogenic differentiation within the 3D aggregates, maintained in culture for up to 21days, and quantified by real time QPCR for osteocalcin. Immunostaining for RUNX2 and osteocalcin, and also histochemical staining with picrosirius red to demonstrate collage type 1 and Alizarin red to demonstrate calcium/mineralisation further demonstrated osteogenic differentiation and revealed regional staining associated with the locations of MPs within the aggregates. We also demonstrated endothelial differentiation within human ES cell-derived aggregates using VEGF loaded MPs. In conclusion, we demonstrate an effective and reliable approach for engineering stem aggregates with definable number of MPs within the 3D cellular structure. We also achieved localised osteogenic and endothelial differentiation associated with MPs releasing encapsulated drug molecules or cytokines directly within the cell aggregate. This provides a powerful tool for controlling and investigating differentiation within 3D cell cultures and has applications to drug delivery, drug discovery, stem cell biology, tissue engineering and regenerative medicine.
A980. WIRELESS GROUND REACTION FORCES AND LOCATIONS ACROSS THE FOOT USING IN-SHOE MEMS PRESSURE SENSORS (2011)

Force profiles across the foot yield information on abnormal kinematics and may be used to indicate pathological changes in the lower limb. However, current technology is limited to tethered systems using wired sensors. This paper outlines a wireless prototype that allows force profile measurement and through an in-shoe monitoring device utilizing custom high-accuracy sensors. Direct measurement of the ground reaction force using a force plate is common practice for use in kinematic studies and is used as an input for mathematical models to predict forces across joints of interest during various activities. Force plates are reasonably accurate but are bulky and only allow one net force measurement at a single location and are not portable. Thus natural patient motion may be modified, intentionally or unintentionally, in order for heelstrike to occur on the force plate. In addition to force magnitude, it is useful to record force location to correlate with kinematics; abnormal kinematics will cause weight-bearing forces to shift across the foot. Current in-shoe pressure measurement devices on the market are plagued by errors up to 30% and require a cumbersome cable out of the shoe to read sensor data. By eliminating all wires, our device enables in-shoe monitoring in a research or clinical environment. The device uses microelectromechanical system (MEMS) capacitive pressure sensors fabricated in a flexible array that attaches to a shoe insole or orthotic. The sensors are concentrated at the heel and forefoot in the prototype design and they exhibit a highly linear response to loading, eliminating the need for constant recalibration. Electronics embedded in the shoe read the entire array of 256 sensors at a rate of 60 Hz. The data is transmitted via Bluetooth at 2.4 GHz to the receiving computer for visualization and analysis. The paper assesses current technology in in-shoe sensing, outlines the device design, and reports initial stages of testing. The prototype developed in this study shows promise for wireless monitoring of ground reaction forces for biomechanics analysis without restricting activity or impeding natural motion.
Recent Development and Remaining Challenges of Iron Sulfide Scale Mitigation in Sour Gas Wells (2019)

Iron sulfide scale deposition can be a significant flow assurance issue in the sour gas production systems. It can deposit along the water flowing path from near wellbore reservoir area to surface equipment, which results in formation damage, causes tubing blockage, interferes with well intervention, and reduces hydrocarbon production. The main objectives of this paper are to review on the new advancements and remaining challenges of iron sulfide scale management in sour gas wells, covering the mechanisms of iron sulfide formation; the mechanical and chemical removal techniques; and the prevention strategies. This paper gives a special emphasis to the different mechanisms of iron sulfide formation during well completion and production stages, especially the sources of ferrous iron for scale deposition. It is essential to understand the root-cause in order to identify and develop suitable technologies to manage the scale problem. The paper also summarizes the latest developments in mechanical methods and chemical dissolvers for the removal of iron sulfide deposited on downhole tubing. The capabilities of the current chemical dissolvers is discussed and the criteria of effective dissolvers are provided to serve as a guide for future development. Then the paper provides an overview of recent developments on iron sulfide prevention technologies and treatment strategies. It differentiates the treatment approaches for corrosion byproduct and scale precipitation and scale inhibitor deployment through continuous injection and squeeze treatment. Finally, the paper outlines the technical gaps and areas for further research and development efforts. This paper provides the latest review on irons sulfide formation and mitigation with an attempt to integrate viable solutions and expose workable practices.
Exposure to Artificial Light at Night and the Consequences for Flora, Fauna, and Ecosystems (2020)

The present review draws together wide-ranging studies performed over the last decades that catalogue the effects of artificial-light-at-night (ALAN) upon living species and their environment. We provide an overview of the tremendous variety of lightdetection strategies which have evolved in living organisms -unicellular, plants and animals, covering chloroplasts (plants), and the plethora of ocular and extra-ocular organs (animals). We describe the visual pigments which permit photo-detection, paying attention to their spectral characteristics, which extend from the ultraviolet into infrared. We discuss how organisms use light information in a way crucial for their development, growth and survival: phototropism, phototaxis, photoperiodism, and synchronization of circadian clocks. These aspects are treated in depth, as their perturbation underlies much of the disruptive effects of ALAN. The review goes into detail on circadian networks in living organisms, since these fundamental features are of critical importance in regulating the interface between environment and body. Especially, hormonal synthesis and secretion are often under circadian and circannual control, hence perturbation of the clock will lead to hormonal imbalance. The review addresses how the ubiquitous introduction of light-emitting diode technology may exacerbate, or in some cases reduce, the generalized ever-increasing light pollution. Numerous examples are given of how widespread exposure to ALAN is perturbing many aspects of plant and animal behaviour and survival: foraging, orientation, migration, seasonal reproduction, colonization and more. We examine the potential problems at the level of individual species and populations and extend the debate to the consequences for ecosystems. We stress, through a few examples, the synergistic harmful effects resulting from the impacts of ALAN combined with other anthropogenic pressures, which often impact the neuroendocrine loops in vertebrates. The article concludes by debating how these anthropogenic changes could be mitigated by more reasonable use of available technology -for example by restricting illumination to more essential areas
Conformational Studies on Tocinamide and Deaminotocinamide by 220 MHz Nuclear Magnetic Resonance Spectroscopy (1972)

The 220 MHz spectra of tocinamide and deaminotocinamide, the ring moieties of oxytocin and of deamino-oxytocin, respectively, were investigated in [U-(2)H]dimethylsulfoxide solution. Extensive decoupling and exchange experiments allow complete spectral assignment and determination of many coupling constants. Circular dichroism spectra assigned a right-hand screw sense to the C-S-S-C group. The conformations of tocinamide and deaminotocinamide are different from each other and from those suggested for the ring portions of oxytocin and deamino-oxytocin. The relationship of this difference to biological activity is commented upon. The importance of the interaction of the side chain with the ring in oxytocin and deamino-oxytocin is emphasized.
Energy Flow in the Information Technology Stack: Introducing the Coefficient of Performance of the Ensemble (2006)

The information technology industry is in the midst of a transformation to lower the cost of operation through consolidation and better utilization of critical data center resources. Successful consolidation necessitates increasing utilization of capital intensive "always-on" data center infrastructure, and reducing the recurring cost of power. A need exists, therefore for an end to end physical model that can be used to design and manage dense data centers and determine the cost of operating a data center. The chip core to the cooling tower model must capture the power levels and thermo-fluids behavior of chips, systems, aggregation of systems in racks, rows of racks, room flow distribution, air conditioning equipment, hydronics, vapor compression systems, pumps and heat exchangers. Earlier work has outlined the foundation for creation of a "smart" data center through use of flexible cooling resources and a distributed sensing and control system that can provision the cooling resources based on the need. This paper shows a common thermodynamic platform which serves as an evaluation and basis for policy based control engine for such a "smart" data center with much broader reach - from chip core to the cooling tower. Computational Fluid Dynamics modeling is performed to determine the computer room air conditioning utilization for a given distribution of heat load and cooling resources in a production data center. Coefficient of performance (COP) of the computer room air conditioning units, based on the level of utilization, is used with COP of other cooling resources in the stack to determine the COP of the ensemble. The ensemble COP represents an overall measure of the performance of the heat removal stack in a data center.
Ultrafast Pump−Probe Studies oftert-Butyl Aroylperbenzoates and Triplet Energy Interception by the O−O Bond (2004)

Photolysis of tert-butyl aroylperbenzoates (BAPs) 1−4 in benzene and acetonitrile at 335 or 267 nm produces the S1 states (λmax ≈ 590 nm) that rapidly intersystem cross (kISC ≈ 1.3 × 1011 s-1) into the triplet states (λmax ≈ 560 nm, τT = 0.6−0.7 ns in benzene). Results from the 267 nm excitation experiments of 1 in acetonitrile suggest the rate of the S2 → S1 internal conversion (kS2→S1 = 1.6 × 1012 s-1) is similar to that of benzophenone and p-iodobenzophenone. The rate of the S1 → T1 intersystem crossing is not affected by the solvent or the excitation wavelength. However, decay of the triplet states of 1 and p-iodobenzophenone is faster in acetonitrile than in benzene. Intramolecular triplet energy dissipation to a repulsive excited triplet state of the O−O bond of BAPs may be a possible mechanism. The results obtained from semiempirical and ab initio calculations have been presented.
Structural System Design (2022)

No AccessInjection Mold Design EngineeringOct 2022Structural System DesignDavid O. KazmerDavid O. KazmerSearch for more papers by this authorhttps://doi.org/10.3139/9781569908921.012SectionsAboutPDF ToolsAdd to FavoritesDownload CitationTrack CitationsCopy LTI LinkPDF key 'share (en)' returned an object instead of string.FacebookTwitterEmailLinkedIn previous chapternext chapter FiguresReferencesRelatedDetails 2022Pages: 449-496Print ISBN: 978-1-56990-891-4eISBN: 978-1-56990-892-1 Copyright & Permissions© 2022 Carl Hanser Verlag GmbH & Co. KGPDF downloadLoading ...