gebhart/patrepeval huggingface dataset:
Site-Directed Mutagenesis Study on the Thermal Stability of a Chimeric PQQ Glucose Dehydrogenase and Its Structural Interpretation (1999)

We have previously reported that a chimeric pyrroloquinoline quinone (PQQ) glucose dehydrogenase (GDH), E97A3, which was made up of 97% of Escherichia coli PQQGDH sequence and 3% of Acinetobacter calcoaceticus PQQGDH, showed increased thermal stability compared with both parental enzymes. Site-directed mutagenesis studies were carried out in order to investigate the role of amino-acid substitution at the C-terminal region, Ser771, of a chimeric PQQGDHs on their thermal stability. A series of Ser771 substitutions of a chimeric PQQGDH, E99A1, confirmed that hydrophobic interaction governs the thermal stability of the chimeric enzymes. Comparison of the thermal denaturation of E. coli PQQGDH and E97A3 followed by far-ultraviolet (UV) circular dichroism (CD) spectroscopy revealed that E97A3 acquired stability at the first step of denaturation, which is reversible, and where no significant secondary structure change was observed. These results suggested that the interaction between C-terminal and N-terminal regions may play a crucial role in maintaining the overall structure of β-propel-ler proteins.
Gonococcal Cervicitis: A Role for Biofilm in Pathogenesis (2008)

Neisseria gonorrhoeae forms a biofilm in flow cells on glass coverslips as well as on primary cervical epithelial cells. Electron microscopic studies of cervical biopsy specimens from 10 patients with culture-proven N. gonorrhoeae infection revealed evidence of biofilm formation in 3 of the biopsy specimens. These biofilms showed gonococci in networks of bacterial membrane within the biofilm structure. This finding was also observed in biofilms formed over glass cover slips and after infection of primary cervical tissue in vitro. The importance of membranous networks in Neisseria biofilm formation was demonstrated with N. gonorrhoeae strain 1291-msbB, which shows a markedly decreased ability to bleb. This mutant formed significantly less biofilm over glass surfaces and cervical epithelial cells, and complementation showed reversion to wild-type biofilms. Gonoccal biofilms, as part of the cervical infection, may be involved in the mechanisms by which asymptomatic infections, persistence, and increased antibiotic resistance occur.
Improved Performance Encapsulation Film Based on Polyolefin (2012)

Encapsulation film plays a key role in the protection of a PV module. Thermal stability, resistance to moisture, stability against UV degradation, and electrical protection are all important components. A polyolefinbased encapsulation film has been developed which has improved performance in these areas as compared to EVA encapsulation film. Optical transmission and color generation of films were studied after accelerated UV exposure. Significantly less generation of yellow color was observed and optical transmission was more stable with the polyolefin film. Lower film water vapor transmission rate results in improved module damp heat performance. Higher film electrical resistivity is shown to correlate with improvements in module PIDresistance. The polyolefin film also has a lower UVcutoff, offering the potential for improved power generation.
System analysis of CCD-based bar code readers (1993)

The basic constraints on the working range of CCD-based bar code readers are analyzed. A comprehensive model that allows the calculation of the optimal working range and its corresponding optical system parameters is derived. Experimental results are found to be in good agreement with theoretical calculations. Finally, comparisons between bar code laser scanners and CCD readers are presented.
Delayed Mismatch Field Latencies in Autism Spectrum Disorder with Abnormal Auditory Sensitivity: A Magnetoencephalographic Study (2017)

Although abnormal auditory sensitivity is the most common sensory impairment associated with autism spectrum disorder (ASD), the neurophysiological mechanisms remain unknown. In previous studies, we reported that this abnormal sensitivity in patients with ASD is associated with delayed and prolonged responses in the auditory cortex. In the present study, we investigated alterations in residual M100 and MMFs in children with ASD who experience abnormal auditory sensitivity. We used magnetoencephalography (MEG) to measure MMF elicited by an auditory oddball paradigm (standard tones: 300 Hz, deviant tones: 700 Hz) in 20 boys with ASD (11 with abnormal auditory sensitivity: mean age, 9.62 ± 1.82 years, 9 without: mean age, 9.07 ± 1.31 years) and 13 typically developing boys (mean age, 9.45 ± 1.51 years). We found that temporal and frontal residual M100/MMF latencies were significantly longer only in children with ASD who have abnormal auditory sensitivity. In addition, prolonged residual M100/MMF latencies were correlated with the severity of abnormal auditory sensitivity in temporal and frontal areas of both hemispheres. Therefore, our findings suggest that children with ASD and abnormal auditory sensitivity may have atypical neural networks in the primary auditory area, as well as in brain areas associated with attention switching and inhibitory control processing. This is the first report of an MEG study demonstrating altered MMFs to an auditory oddball paradigm in patients with ASD and abnormal auditory sensitivity. These findings contribute to knowledge of the mechanisms for abnormal auditory sensitivity in ASD, and may therefore facilitate development of novel clinical interventions.
Fabrication and Properties of an Anisotropic TiO 2 Dielectric Composite (2006)

Thermoplastic co‐extrusion was employed to fabricate an anisotropic dielectric composite based on titanium dioxide with a microcellular architecture with 50 μm macropore channels aligned to create unidirectional porosity. The resulting channeled structure exhibits artificial anisotropic dielectric properties, with the dielectric relative permittivity being 9.6 transverse to the channels and 90 in the longitudinal direction.
<title>Using self-authentication and recovery images for error concealment in wireless environments</title> (2001)

Handling packet loss or delay in the mobile and/or Internet environment is usually a challenging problem for multimedia transmission. Using connection-oriented protocol such as TCP may introduce intolerable time delay in re-transmission. Using datagram-oriented protocols such as UDP may cause partial representation in case of packet loss. In this paper, we propose a new method of using our self-authentication-and-recovery images (SARI) to do the error detection and concealment in the UDP environment. The lost information in a SARI image can be approximately recovered based on the embedded watermark, which includes the content-based authentication information and recovery information. Images or video frames are watermarked in a priori such that no additional mechanism is needed in the networking or the encoding process. Because the recovery is not based on adjacent blocks, the proposed method can recover the corrupted area even though the information loss happen in large areas or high variant areas. Our experiments show the advantages of such technique in both transmission time saving and broad application potentials.
Ring-Closing Metathesis in Methanol and Water (1998)

The ring-closing metathesis (RCM) of acyclic dienes in both methanol and water has been achieved through the use of water-soluble ruthenium alkylidenes. These alkylidenes react readily with acyclic olefins in protic solvents, but they do not cyclize α,ω-dienes because of the instability of the resulting methylidene. Successful cyclization has been achieved through simple substrate modificationincorporation of an olefin substituent allows cyclization to proceed in good yield. A methyl-substituted substrate was cyclized in 60% conversion in methanol, and the incorporation of a phenyl substituent resulted in nearly quantitative cyclization. Phenyl-substituted substrates are best suited for the reaction, as a more stable, active catalyst is regenerated upon each catalyst turnover. Using this methodology, 90% conversion of a water-soluble substrate to a substituted cyclopentene has been achieved in aqueous solution. This methodology has also been successfully applied to increase RCM yields in organic solvents.
Preparation and reaction of dilithio-2,4-oxazolidinedione with .alpha.-halo ketones. A versatile synthesis of 3-hydroxy-2(5H)-furanones (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPreparation and reaction of dilithio-2,4-oxazolidinedione with .alpha.-halo ketones. A versatile synthesis of 3-hydroxy-2(5H)-furanonesArie ZaskCite this: J. Org. Chem. 1992, 57, 16, 4558–4560Publication Date (Print):July 1, 1992Publication History Published online1 May 2002Published inissue 1 July 1992https://pubs.acs.org/doi/10.1021/jo00042a045https://doi.org/10.1021/jo00042a045research-articleACS PublicationsRequest reuse permissionsArticle Views326Altmetric-Citations20LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Transcatheter aortic root replacement with chimney grafts for coronary perfusion: a preliminary test in a three-dimensional-printed root model (2020)

In this preliminary study, chimney grafts for transcatheter aortic root repair provided 830% of target flow in the right coronary (-31% of free flow) and 414% of target flow in the left coronary (-42% of free flow) which is more than sufficient for both coronaries in real-life conditions. The potential of this approach should be further explored with specifically designed endografts.
Colloidal Polyelectrolyte complexes for the delivery of pharmaceuticals (2012)

International audience
Characterization of the MSE Region under a Total Power Budget for Asynchronous Two-Way Relay Networks (2019)

We consider a single-carrier bi-directional asynchronous relay network, where two single-antenna transceivers exchange information with the help of multiple single-antenna amplify-and-forward relays. This network is asynchronous in the sense that the signal transmitted by any of the two transceivers arrives at the other transceiver through different relaying paths with different delays, thereby materializing a multi-path channel which can cause inter-symbol-interference in the received signal. In such a network, assuming linear post-channel block equalization is used at the receiver front-end of the two transceivers, this paper presents a rigorous characterization of the region of the mean-squared errors (MSEs) of the symbol estimates at the two transceivers under a total power budget.
Suppression of the GnRH Pulse Generator by Neurokinin B Involves a κ-Opioid Receptor-Dependent Mechanism (2012)

Neurokinin B (NKB) and its receptor (NK3R) are coexpressed with kisspeptin, Dynorphin A (Dyn), and their receptors [G-protein-coupled receptor-54 (GPR54)] and κ-opioid receptor (KOR), respectively] within kisspeptin/NKB/Dyn (KNDy) neurons in the hypothalamic arcuate nucleus (ARC), the proposed site of the GnRH pulse generator. Much previous research has employed intracerebroventricular (icv) administration of KNDy agonists and antagonists to address the functions of KNDy neurons. We performed a series of in vivo neuropharmacological experiments aiming to determine the role of NKB/NK3R signaling in modulating the GnRH pulse generator and elucidate the interaction between KNDy neuropeptide signaling systems, targeting our interventions to ARC KNDy neurons. First, we investigated the effect of intra-ARC administration of the selective NK3R agonist, senktide, on pulsatile LH secretion using a frequent automated serial sampling method to obtain blood samples from freely moving ovariectomized 17β-estradiol-replaced rats. Our results show that senktide suppresses LH pulses in a dose-dependent manner. Intra-ARC administration of U50488, a selective KOR agonist, also caused a dose-dependent, albeit more modest, decrease in LH pulse frequency. Thus we tested the hypothesis that Dyn/KOR signaling localized to the ARC mediates the senktide-induced suppression of the LH pulse by profiling pulsatile LH secretion in response to senktide in rats pretreated with nor-binaltorphimine, a selective KOR antagonist. We show that nor-binaltorphimine blocks the senktide-induced suppression of pulsatile LH secretion but does not affect LH pulse frequency per se. In order to address the effects of acute activation of ARC NK3R, we quantified (using quantitative RT-PCR) changes in mRNA levels of KNDy-associated genes in hypothalamic micropunches following intra-ARC administration of senktide. Senktide down-regulated expression of genes encoding GnRH and GPR54 (GNRH1 and Kiss1r, respectively), but did not affect the expression of Kiss1 (which encodes kisspeptin). We conclude that NKB suppresses the GnRH pulse generator in a KOR-dependent fashion and regulates gene expression in GnRH neurons.
Interaction of Escherichia coli Thioredoxin with the Bacteriophage T7 Polymerase, Helicase/Primase at the Replication Fork (2007)

Escherichia coli thioredoxin (Trx) is the only host protein essential for bacteriophage T7 DNA replication. It binds tightly to the gene 5 DNA polymerase (gp5) (Kd= 5 nM) and increases its processivity ~200 fold. Crystal structure of gp5/Trx bound to a primer‐template reveals that Trx interacts with a unique flexible 71 amino acid segment in the thumb domain of gp5. Two basic loops (A and B) in this thioredoxin binding domain (TBD) interact with the acidic C‐terminal tails of the helicase domain of gene 4 protein (gp4) and gene 2.5 i.e, single‐strand DNA binding protein (gp2.5). This interaction is enhanced by the presence of Trx. Elimination of basic residues in loops A and B decreases binding of gp5/trx to gp4 and gp2.5, but elimination of charges in both loops is required to prevent binding. Several basic residues, proximal to loops A and B, are also present on Trx. We have substituted glutamate for two of these residues, lysines 36 and 57, located proximal to loop A. Gp5 containing a normal loop A and a loop B where basic residues have been eliminated binds to the altered Trx but the complex binds gp4 90‐fold less tightly than does the wild‐type protein. This weak interaction is similar to that observed when wild‐type Trx binds to gp5 with alteration in both loops A and B. We propose that loop B is essential for interaction with gp4 during leading strand synthesis and that loop A provides the conformational changes required for this interaction.
N‐bromo‐hydantoin grafted polystyrene beads: Synthesis and nano‐micro beads characteristics for achieving controlled release of active oxidative bromine and extended microbial inactivation efficiency (2015)

ABSTRACT N ‐bromo‐hydantoin and N ‐bromo‐5,5′‐dimethylhydantoin conjugated polystyrene beads were synthesized from chloromethyl polystyrene beads which differ in their particles size, crosslinking, nano‐micro porosity, and tunnels size on the surface, in order to study the effect of these parameters on oxidative halogen release and resultant activity, for water purification applications. The synthesized beads were characterized using elemental analysis, FT‐IR, solid state 13 C‐NMR, and scanning electron microscope (SEM). The conjugation yield and kinetics in different solvents and bromine loading capacity were studied. The N ‐bromoamine polystyrene beads were tested for water decontamination according to NSF 231 protocol. The release of active bromine was analyzed by spectrophotometer using a DPD‐1 kit and also studied the antimicrobial activity against Escherichia coli and MS2 phages. Bead's nano‐micro characteristics were found critical for oxidative halogen release control: rate stabilization and modulation, extension and also influences antimicrobial activity. The synthesized beads exhibited extended and stable release of bromine, 6 and 4 log reduction for E. coli and MS2, respectively for 250 L of passing contaminated water. Thus, N ‐halamine hydantoins conjugated polystyrenes, chemically or kinetically release modified should have applications as disinfectants in water purification systems as well as medical field. © 2015 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2016 , 54 , 596–610
OpenStereo Converting Satellite Image Pairs into Anaglyph Stereoscopic Views (2007)

Recent years have promoted important evolution in remote sensing, specially taking into consideration quality improvements on satellites that are now capable of obtaining more detailed and accurate observation and producing stereoscopic views. Following market laws, once the level of detail increases the associated costs also rise. In order to take advantage of modest dataset acquisition costs the OpenStereo library (a free, open source, multiplatform, flexible and robust library for stereoscopy) is applied with the purpose of converting satellite images and similar types of geodata acquirers into stereoscopic views.
Single-Cell RNAseq Analysis Reveals Robust, Anti-PD-1-Mediated Increase of Immune Infiltrate in Metastatic Castration-Sensitive Prostate Cancer (2022)

SUMMARY Compared to other malignancies, the tumor microenvironment (TME) of primary and castration-resistant prostate cancer (CRPC) is relatively devoid of immune infiltrates. While androgen deprivation therapy (ADT) induces a complex immune infiltrate in localized prostate cancer, both in animal models and humans, the TME composition of metastatic, castration-sensitive prostate cancer (mCSPC) is relatively unknown and the effects of ADT and other treatments are poorly characterized in this context. To address this challenge, we analyzed metastatic sites from patients enrolled on a phase 2 clinical trial ( NCT03951831 ), in which men were treated with standard-of-care chemo-hormonal therapy with anti-PD-1 immunotherapy, at the single cell level. Longitudinal protein activity-based analysis of TME subpopulations identified immune subpopulations conserved across multiple metastatic sites, their dynamic, treatment-mediated evolution, and associated clinical response features. Our study revealed a therapy-resistant, transcriptionally distinct tumor subpopulation, which comprises an increasing number of cells in treatment-refractory patients, and identified several druggable targets in both tumor and immune cells as candidates to advance treatment and improve outcomes for patients with mCSPC.
Resampling Based Multiple Testing Procedure Controlling Tail Probability of the Proportion of False Positives (2005)

Suppression of unwanted motor responses can be disrupted by Parkinson's disease. People with Parkinson's (PwP) can show maladaptive reward-driven behaviours in the form of impulse control behaviours, which are associated with the use of the dopaminergic treatments used to alleviate the motor symptoms of the disease. However, the effects of Parkinson's itself on impulsive behaviour and control are unclear-empirical studies have yielded mixed findings, and some imaging studies have shown a functional deficit in the absence of a measurable change in behaviour. Here, we investigated the effects of Parkinson's on response activation and control by studying the dynamics of response in standard inhibitory control tasks-the Stop Signal and Simon tasks-using a continuous measure of response force. Our results are largely in favour of the conclusion that response inhibition appears to be intact in PwP, even when using a more sensitive measure of behavioural control relative to traditional button-press measures. Our findings provide some clarity as to the effects of Parkinson's on response inhibition and show continuous response force measurement can provide a sensitive means of detecting erroneous response activity in PwP, which could also be generalised to studying related processes in other populations.
304 Tb/s transmission over transpacific distance using 200 Gb/s and dual wavelength 400 Gb/s 16QAM at 60 b/s/Hz spectral efficiency (2014)

Using single-stage C-band EDFAs equalized to 41 nm, we transmit 152 x 200 Gb/s PDM 16QAM channels with 6.0 b/s/Hz spectral efficiency over 9,748 km enabled by Nyquist spectral shaping and digital back propagation. 76 x 400 Gb/s channels are also transmitted over 8,665 km detecting two 200 Gb/s channels simultaneously using a single wideband receiver. Digital back propagation benefit versus channel pre-emphasis, transmission distance and wavelength are experimentally investigated.
Hydrogel Containing Solid Lipid Nanoparticles Loaded with Argan Oil and Simvastatin: Preparation, In Vitro and Ex Vivo Assessment (2022)

Transdermal hydrogels have the potential to improve therapeutic outcomes via enhancing bioavailability and reducing toxicity associated with oral delivery. The goal of the present study was to formulate and optimise argan oil loaded transdermal hydrogel containing lipid nanoparticles. The high pressure homogenization (HPH) method was utilised to fabricate Simvastatin loaded solid lipid nanoparticles (SIM-SLNs) with precirol ATO 5 as a lipid core and Poloxamer 407 (P407) to stabilise the core. The optimised nanoformulation was characterised for its particle diameter, zeta potential, surface morphology, entrapment efficiency, crystallinity and molecular interaction. Furthermore, transdermal hydrogel was characterised for physical appearance, rheology, pH, bio adhesion, extrudability, spreadability and safety profile. In vitro and ex vivo assays were executed to gauge the potential of SLNs and argan oil for transdermal delivery. The mean particle size, zeta potential and polydispersity index (PDI) of the optimised nanoparticles were 205 nm, -16.6 mV and 0.127, respectively. Crystallinity studies and Fourier transform infrared (FTIR) analysis revealed no molecular interaction. The in vitro release model explains anomalous non-Fickian release of drug from matrix system. Ex vivo skin penetration studies conducted through a fluorescence microscope confirmed penetration of the formulation across the stratum corneum. Hydrogel plays a crucial role in controlling the burst release and imparting the effect of argan oil as hypolipidemic agent and permeation enhancer.