Human Anti-IgE Monoclonal Antibody Blocks Passive Sensitization of Human and Rhesus Monkey Bladder (1997)

No AccessJournal of UrologyInvestigative Urology1 Feb 1997Human Anti-IgE Monoclonal Antibody Blocks Passive Sensitization of Human and Rhesus Monkey Bladder R. Saban, M. Haak-Frendscho, M. Zine, L.G. Presta, D.E. Bjorling, and P. Jardieu R. SabanR. Saban More articles by this author , M. Haak-FrendschoM. Haak-Frendscho More articles by this author , M. ZineM. Zine More articles by this author , L.G. PrestaL.G. Presta More articles by this author , D.E. BjorlingD.E. Bjorling More articles by this author , and P. JardieuP. Jardieu More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)65251-0AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail IgE antibodies may play an important role in noninfectious urinary inflammation, including interstitial cystitis (IC). In this study, urinary bladder strips were passively sensitized for 20 hours with serum from a ragweed-sensitive patient in the absence or presence of an anti-human IgE monoclonal antibody (MaE11) at 0, 1-, or 5-fold IgE concentration. The urinary bladder strips then were suspended in a superfusion apparatus for measurement of contraction and histamine release in response to antigen E (AgE) challenge. Non-sensitized tissues did not react to AgE challenge, whereas AgE challenge of passively-sensitized tissues resulted in time-dependent bladder contraction and histamine release. MaE11 abolished AgE-induced contraction and histamine release in a concentration-dependent manner. The safety of MaE11 was confirmed by its failure to contract or release histamine from passively-sensitized bladder tissues. The results of this study suggest that MaE11 may have immunotherapeutic benefit for amelioration of IgE-mediated diseases of the urinary system. References 1 : Sjogren's syndrome in patients with interstitial cystitis. J. Rheumatol.1993; 20: 962. Google Scholar 2 : Abnormal urothelial HLA-DR expression in interstitial cystitis. Clin. Exp. Immunol.1992; 87: 450. Google Scholar 3 : Evidence for urothelial cell activation in interstitial cystitis. J. Urol.1993; 149: 470. Link, Google Scholar 4 : Cellular immunity in interstitial cystitis. J. Urol.1991; 145: 274. Link, Google Scholar 5 : The enigma of interstitial cystitis-an autoimmune disease? Br. J. Urol.1989; 63: 58. Google Scholar 6 : Interstitial cystitis: report of 223 cases (204 women and 19 men). J. Urol.1949; 61: 291. Link, Google Scholar 7 : Interstitial cystitis and related syndromes. In: Campbell's Urology. Edited by . Philadelphia: W. B. Saunders Co.1991: 982. Google Scholar 8 : Painful bladder disease: clinical and pathoanatomical differences in 115 patients. J. Urol.1987; 138: 500. Link, Google Scholar 9 : Experimental autoimmune cystitis: a potential murine model for ulcerative interstitial cystitis. J. Urol.1992; 148: 1951. Link, Google Scholar 10 : Epidemiology of interstitial cystitis. Urol. Clin. North Am.1994; 21: 7. Crossref, Medline, Google Scholar 11 : The role of mast cells in interstitial cystitis. Urol. Clin. North Am.1994; 21: 41. Google Scholar 12 : The etiology of interstitial cystitis. Urol. Clin. North Am.1994; 21: 21. Google Scholar 13 : Mast cell-derived mediators: structural and functional diversity and regulation of expression. In: Molecular Aspects for the Acute Allergic Reactions. Edited by . New York: Plenum Press1976: 293. Google Scholar 14 : Biochemical analysis of initial triggering events of IgE mediated histamine release from human mast cells. J. Immunol.1983; 130: 2357. Google Scholar 15 : Inhibition of mast cell sensitization in vitro by a human immunoglobulin e-chain fragment synthesized in Escherichia coli. Eur. J. Immunol.1985; 15: 966. Google Scholar 16 : Inhibition of the Prausnitz-Kustner reaction by an immunoglobulin e-chain fragment synthesized in E. coli. Nature1985; 315: 577. Google Scholar 17 : The mast cell binding site on human immunoglobulin E. Nature (London)1988; 331: 180. Google Scholar 18 : Inhibition of IgE binding to mast cells and basophils by monoclonal antibodies to murine IgE. Eur. J. Immunol.1984; 14: 799. Google Scholar 19 : Humanization of an antibody directed against immunoglobulin E. J. Immunol.1993; 151: 2623. Google Scholar 20 : In vitro passive sensitization of guinea pig, Rhesus monkey, and human bladders as a model of noninfectious cystitis. J. Urol.1994; 152: 1603. Abstract, Google Scholar 21 : Studies on the relationship between contraction and mediator release produced by ovalbumin in superfused trachea isolated from the actively sensitized guinea pig. J. Pharmacol. Exp. Ther.1984; 230: 534. Google Scholar 22 : Human IgE receptor alpha chain-IgG chimera blocks passive cutaneous anaphylaxis reaction in vivo. J. Immunol.1993; 151: 351. Google Scholar 23 : An automated continuous-flow system for the extraction and fluorometric analysis of histamine. Anal. Biochem.1974; 57: 383. Google Scholar 24 Fundamentals of Biostatistics. Edited by . Boston: PWS Publishers1990: 442. Google Scholar 25 : Human IgE receptor alpha chain-IgG chimera blocks passive sensitization of human and Rhesus monkey lung. J. Allergy Clin. Immunol.1994; 94: 836. Google Scholar 26 : Response of the isolated guinea pig bladder to exogenous and endogenous leukotrienes. J. Urol.1994; 152: 1281. Link, Google Scholar 27 : Experimental model for the study of bladder mast cell degranulation and smooth muscle contraction. Sem. Urol.1991; 9: 88. Google Scholar 28 : Relationships between epitopes on IgE recognized by defined monoclonal antibodies and by the FC epsilon receptor on basophils. J. Immunol.1986; 136: 588. Google Scholar 29 : Elevated mast cell tryptase in the urine of patients with interstitial cystitis. Br. J. Urol.1995; 76: 94. Google Scholar 30 : Activation of bladder mast cells in interstitial cystitis: a light and electron microscopic study. J. Urol.1995; 153: 629. Link, Google Scholar 31 : Intragranular activation of bladder mast cells and their association with nerve processes in interstitial cystitis. Br. J. Urol.1996; 77: 41. Google Scholar Smooth Muscle Laboratory, Department of Surgical Sciences, School of Veterinary Medicine University of Wisconsin, Madison, Wisconsin; Promega Corp., Madison, Wisconsin 53711; and the Departments of Protein Engineering and Immunology, Genentech Inc., San Francisco, California.© 1997 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 157 Issue 2 February 1997 Page: 689-693 Advertisement Copyright & Permissions© 1997 by American Urological Association, Inc.Metrics Author Information R. Saban More articles by this author M. Haak-Frendscho More articles by this author M. Zine More articles by this author L.G. Presta More articles by this author D.E. Bjorling More articles by this author P. Jardieu More articles by this author Expand All Advertisement PDF downloadLoading ...