gebhart/patrepeval huggingface dataset:
Non‐transcriptional regulation of NLRP3 inflammasome signaling by IL‐4 (2015)

Th2 cytokine IL-4 has been previously shown to suppress the production of proinflammatory cytokines in monocytes. However, the underlying molecular mechanism by which IL-4 signaling antagonizes proinflammatory responses is poorly characterized. In particular, whether IL-4 can modulate inflammasome signaling remains unknown. Here, we provide evidence that IL-4 suppresses NLRP3-dependent caspase-1 activation and the subsequent IL-1β secretion but does not inhibit absent in melanoma 2 (AIM2)- or NLRC4 (NOD-like receptor family, CARD domain-containing 4)-dependent caspase-1 activation in THP-1 and mouse bone marrow-derived macrophages. Upon lipopolysaccharide (LPS) or LPS/ATP stimulation, IL-4 markedly inhibited the assembly of NLRP3 inflammasome, including NLRP3-dependent ASC (apoptosis-associated speck-like protein containing a caspase recruitment domain) oligomerization, NLRP3-ASC interaction and NLRP3 speck-like oligomeric structure formation. The negative regulation of NLRP3 inflammasome by IL-4 was not due to the impaired mRNA or protein production of NLRP3 and proinflammatory cytokines. Supporting this observation, IL-4 attenuated NLRP3 inflammasome activation even in reconstituted NLRP3-expressing macrophages in which NLRP3 expression is not transcriptionally regulated by TLR-NF-κB signaling. Furthermore, the IL-4-mediated suppression of NLRP3 inflammasome was independent of STAT6-dependent transcription and mitochondrial reactive oxygen species (ROS). Instead, IL-4 inhibited subcellular redistribution of NLRP3 into mitochondria and microtubule polymerization upon NLRP3-activating stimulation. Our results collectively suggest that IL-4 could suppress NLRP3 inflammasome activation in a transcription-independent manner, thus providing an endogenous regulatory machinery to prevent excessive inflammasome activation.
<title>The Holowriter</title> (1983)

Computer generated holograms have not been widely used for metrology and nondestructive testing because the required accuracy was beyond the state of the art and the cost of the best available hologram writer was prohibitive. Both of these barriers have now been removed. We have designed and built an adequate writer (the holowriter) and made it available at no cost (except for labor) to all workers on U.S. Government contracts. This paper describes the Holowriter design and performance.
Generation of Kerr combs centered at 45  μm in crystalline microresonators pumped with quantum-cascade lasers (2015)

We report on the generation of mid-infrared Kerr frequency combs in high-finesse CaF2 and MgF2 whispering-gallery-mode resonators pumped with continuous-wave room-temperature quantum cascade lasers. The combs were centered at 4.5 μm, the longest wavelength to date. A frequency comb wider than one half of an octave was demonstrated when approximately 20 mW of pump power was coupled to an MgF2 resonator characterized with quality factor exceeding 10(8).
Detection of ions released from S-PRG fillers and their modulation effect (2010)

The purpose of this study was to analyze the ions released from a surface pre-reacted glass ionomer (S-PRG) filler in distilled water or lactic acid solution. S-PRG filler was mixed with either solution at 1000:1, 100:1, 10:1, and 1:1 ratios by weight. By means of inductively coupled plasma atomic emission spectroscopy and a fluoride electrode, elements released from S-PRG filler were identified to be Al, B, Na, Si, Sr, and F. To investigate the effect of solution pH on ion release, the pH values of the solutions before mixing and after 24 hours' mixing with S-PRG were measured. After 24 hours' mixing, the pH values of solutions at all ratios became more neutral or weakly alkaline regardless of their initial pH levels before mixing. In conclusion, results showed that S-PRG filler released several types of ions, and that ion release was influenced by the mixing ratio of the solution rather than the initial pH of the solution.
On the fabrication of thin-film artificial metal grid resonator antenna arrays using deep x-ray Lithography (2020)

Abstract In this paper, a unique microfabrication approach for designing high permittivity, high gain and wideband thin-film artificial metal grid dielectric resonator antenna (GDRA) arrays for mm-wave applications is presented. Fabrication of the GDRAs with a strip template frame is based on deep-x-ray lithography (DXRL) using a 500 µ m thick polymethylmethacrylate (PMMA) layer as the photoresist. The simple low-cost fabricated DXRL mask for exposure consisted of gold absorbers patterned by direct-write ultraviolet (UV) laser lithography on a carbon substrate. To avoid the trapping of gas bubbles in the dense, high aspect ratio (HAR) deep grid structures, a novel development approach was introduced utilizing in-vacuum, room temperature dip development to facilitate the collapse of forming gas bubbles. The developed voids were subsequently electroplated to a nickel thickness of 300 µ m to obtain high permittivity artificial dielectrics. The paper describes the entire fabrication sequence. Also, the x-ray mask and DXRL structures are examined for dimensional accuracy and structural quality. This strip template frame approach has superior performance in terms of impedance bandwidth, gain and radiation efficiency as compared to the previously published solid template frame approach. For the purpose of the demonstration, a four-element GDRA array sample was fabricated and tested. A wide measured impedance bandwidth of 5 GHz from 58 GHz to 63 GHz and measured broadside radiation pattern with a peak gain of 10.9 dBi was achieved.
EFFECT OF IN VITRO TREATMENT OF ISOLATED HUMAN ISLETS WITH HUMAN ALPHA1-ANTITRYPSIN (2008)

Balamurugan, A N.1; Lewis, E C.2; Wiseman, A C.1; Pugazhenthi, S1; Sarkar, S A.1; Dinarello, C A.2; Gill, R G.1 Author Information
Incorporation of Fluorotyrosines into Ribonucleotide Reductase Using an Evolved, Polyspecific Aminoacyl-tRNA Synthetase (2011)

Tyrosyl radicals (Y·s) are prevalent in biological catalysis and are formed under physiological conditions by the coupled loss of both a proton and an electron. Fluorotyrosines (F(n)Ys, n = 1-4) are promising tools for studying the mechanism of Y· formation and reactivity, as their pK(a) values and peak potentials span four units and 300 mV, respectively, between pH 6 and 10. In this manuscript, we present the directed evolution of aminoacyl-tRNA synthetases (aaRSs) for 2,3,5-trifluorotyrosine (2,3,5-F(3)Y) and demonstrate their ability to charge an orthogonal tRNA with a series of F(n)Ys while maintaining high specificity over Y. An evolved aaRS is then used to incorporate F(n)Ys site-specifically into the two subunits (α2 and β2) of Escherichia coli class Ia ribonucleotide reductase (RNR), an enzyme that employs stable and transient Y·s to mediate long-range, reversible radical hopping during catalysis. Each of four conserved Ys in RNR is replaced with F(n)Y(s), and the resulting proteins are isolated in good yields. F(n)Ys incorporated at position 122 of β2, the site of a stable Y· in wild-type RNR, generate long-lived F(n)Y·s that are characterized by electron paramagnetic resonance (EPR) spectroscopy. Furthermore, we demonstrate that the radical pathway in the mutant Y(122)(2,3,5)F(3)Y-β2 is energetically and/or conformationally modulated in such a way that the enzyme retains its activity but a new on-pathway Y· can accumulate. The distinct EPR properties of the 2,3,5-F(3)Y· facilitate spectral subtractions that make detection and identification of new Y·s straightforward.
Central levels of tryptophan metabolites in subjects with bipolar disorder (2020)

The kynurenine pathway of tryptophan degradation produces several neuroactive metabolites such as kynurenic acid (KYNA), quinolinic acid (QUIN), and picolinic acid (PIC) thought to be involved in the pathophysiology of psychosis, major depression, and suicidal behavior. Here, we analyzed cerebrospinal fluid (CSF) concentrations of tryptophan, kynurenine, KYNA, QUIN, and PIC utilizing ultra-performance liquid chromatography - tandem mass spectrometry system (UPLC-MS/MS) in persons with bipolar disorder (n = 101) and healthy controls (n = 80) to investigate if the metabolites correlated with depressive symptoms or to the history of suicidal behavior. Furthermore, we analyzed if genetic variants of the enzyme amino-β-carboxymuconate-semialdehyde-decarboxylase (ACMSD) were associated with the CSF concentrations of PIC and QUIN. We found that CSF KYNA and PIC concentrations, as well as the kynurenine/tryptophan ratio were increased in bipolar disorder compared with controls. CSF PIC concentrations were lower in subjects with a history of suicidal behavior than those without, supporting the hypothesis that low CSF PIC is a marker of vulnerability for suicidality. Bipolar subjects taking antidepressants had higher CSF concentrations of kynurenine and KYNA than subjects not given these medications. A negative association was found between a genetic variant of ACMSD and the ratio of PIC/QUIN, indicating that a polymorphism in ACMSD is associated with excess of QUIN formation at the expense of PIC. The present results confirm that the kynurenine pathway is activated in bipolar disorder, and suggest that shifting the activity of the kynurenine pathway away from QUIN production towards a production of KYNA and PIC might be a beneficial therapeutic strategy.
Drop impacts on electrospun nanofiber membranes (2012)

This work reports a systematic study of drop impacts of polar and non-polar liquids onto different electrospun nanofiber membranes (of 8–10 μm thickness and pore sizes of 3–6 μm) with an increasing degree of hydrophobicity. The liquids studied were water, FC 7500 (Fluorinert fluid) and hexane. The nanofibers used were electrospun from polyacrylonitrile (PAN), nylon 6/6, polycaprolactone (PCL) and Teflon. It was found that for any liquid/fiber pair there exists a threshold impact velocity (∼1.5 to 3 m s−1) above which water penetrates membranes irrespective of their hydrophobicity. The other liquids (FC 7500 and hexane) penetrate the membranes even more easily. The low surface tension liquid, FC 7500, left the rear side of sufficiently thin membranes as a millipede-like system of tiny jets protruding through a number of pores. For such a high surface tension liquid as water, jets immediately merged into a single bigger jet, which formed secondary spherical drops due to capillary instability. No mechanical damage to the nanofiber mats after liquid perforation was observed. A theoretical estimate of the critical membrane thickness sufficient for complete viscous dissipation of the kinetic energy of penetrating liquid is given and corroborated by the experimental data.
Synthesis of the brevetoxin B IJK ring system (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of the brevetoxin B IJK ring systemK. C. Nicolaou, C. K. Hwang, and M. E. DugganCite this: J. Am. Chem. Soc. 1989, 111, 17, 6682–6690Publication Date (Print):August 1, 1989Publication History Published online1 May 2002Published inissue 1 August 1989https://pubs.acs.org/doi/10.1021/ja00199a031https://doi.org/10.1021/ja00199a031research-articleACS PublicationsRequest reuse permissionsArticle Views1163Altmetric-Citations92LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
BFT Protocols for Heterogeneous Resource Allocations in Distributed SDN Control Plane (2019)

Distributed Software Defined Networking (SDN) controllers aim to solve the issue of single-point-of-failure and improve the scalability of the control plane. Byzantine and faulty controllers, however, may enforce incorrect configurations and thus endanger the control plane correctness. Multiple Byzantine Fault Tolerance (BFT) approaches relying on Replicated State Machine (RSM) execution have been proposed in the past to cater for this issue. The scalability of such solutions is, however, limited. Additionally, the interplay between progressing the state of the distributed controllers and the consistency of the external reconfigurations of the forwarding devices has not been thoroughly investigated. In this work, we propose an agreement-and-execution group-based approach to increase the overall throughput of a BFT-enabled distributed SDN control plane. We adapt a proven sequencing-based BFT protocol, and introduce two optimized BFT protocols that preserve the uniform agreement, causality and liveness properties. A state-hashing approach which ensures causally ordered switch reconfigurations is proposed, that enables an opportunistic RSM execution without relying on strict sequencing. The proposed designs are implemented and validated for two realistic topologies, a path computation application and a set of KPIs: switch reconfiguration (response) time, signaling overhead, and acceptance rates. We show a clear decrease in the system response time and communication overhead with the proposed models, compared to a state-of-the-art approach.
On the initialization methods of an exterior point algorithm for the assignment problem (2009)

In this paper, we present a theoretical investigation and an extensive computational study of exterior point simplex algorithm (EPSA) initialization methods for the assignment problem (AP). We describe the exterior point algorithm using three different initialization methods. Effective implementations are explored for each initialization method. Then we perform an experimental evaluation on a large set of benchmark problems from the TSPLib 95 and OR Library collections. The results obtained demonstrate the advantages of the three initialization methods. Finally, we give a theoretical justification of the initialization methods efficiency. We explain theoretically the computational ranking for these methods.
Reliability-Oriented Design of Modular Multilevel Converters for Medium-Voltage STATCOM (2019)

Modular multilevel converters (MMC) are complex systems, composed of many elements, and exposed to critical load demands in some cases. Thereby, a detailed design of its components is of preeminent importance to achieve a high system-level reliability. However, the high number of devices challenges the tradeoff between cost and reliability. This article, introduces a reliability-oriented design methodology, based on the cost to achieve a predefined unreliability level (U <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">∞</sub> ). A flowchart presents the main steps of the process, including the mission profile definition, selection of power devices, thermal modeling, reliability modeling, and the reliability-oriented selection. To evaluate the proposed methodology, a case study considering 17 MVA/13.8 kV MMC-static synchronous compensator (STATCOM) with a real mission profile data is conducted. A U <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">∞</sub> - cost map is introduced to compare various design solutions, based on power devices of different voltage classes and current capabilities.
ChemInform Abstract: XtalFluor‐E, an Efficient Coupling Reagent for Amidation of Carboxylic Acids. (2013)

Abstract Aromatic and aliphatic carboxylic acids are easily converted into the corresponding amides by reaction with various amines in the presence of XtalFluor‐E (XTFE) as an activator.
MET Gene Copy Number Alterations and Expression of MET and Hepatocyte Growth Factor Are Potential Biomarkers in Angiosarcomas and Undifferentiated Pleomorphic Sarcomas (2015)

Soft tissue sarcomas are a heterogeneous group of tumors with many different subtypes. In 2014 an estimated 12,020 newly diagnosed cases and 4,740 soft tissue sarcoma related deaths can be expected in the United States. Many soft tissue sarcomas are associated with poor prognosis and therapeutic options are often limited. The evolution of precision medicine has not yet fully reached the clinical treatment of sarcomas since therapeutically tractable genetic changes have not been comprehensively studied so far. We analyzed a total of 484 adult-type malignant mesenchymal tumors by MET fluorescence in situ hybridization and MET and hepatocyte growth factor immunohistochemistry. Eleven different entities were included, among them the most common and clinically relevant subtypes and tumors with specific translocations or complex genetic changes. MET protein expression was observed in 2.6% of the cases, all of which were either undifferentiated pleomorphic sarcomas or angiosarcomas, showing positivity rates of 14% and 17%, respectively. 6% of the tumors showed hepatocyte growth factor overexpression, mainly seen in undifferentiated pleomorphic sarcomas and angiosarcomas, but also in clear cell sarcomas, malignant peripheral nerve sheath tumors, leiomyosarcomas and gastrointestinal stromal tumors. MET and hepatocyte growth factor overexpression were significantly correlated and may suggest an autocrine activation in these tumors. MET FISH amplification and copy number gain were present in 4% of the tumors (15/413). Two samples, both undifferentiated pleomorphic sarcomas, fulfilled the criteria for high level amplification of MET, one undifferentiated pleomorphic sarcoma reached an intermediate level copy number gain, and 12 samples of different subtypes were categorized as low level copy number gains for MET. Our findings indicate that angiosarcomas and undifferentiated pleomorphic sarcomas rather than other frequent adult-type sarcomas should be enrolled in screening programs for clinical trials with MET inhibitors. The screening methods should include both in situ hybridization and immunohistochemistry.
Inkjet Injection of DNA Droplets for Microchannel Array Electrophoresis (2012)

We demonstrated DNA droplets could be injected with an inkjet injector for microchannel array electrophoresis and attained high throughput analysis of biomolecules. This injection method greatly reduced both analysis time and sample amount, compared with a conventional microchip electrophoresis method, and allowed high parallelization of a microchannel array on a small substrate. Since we do not need to use complicated electric programs or microchannel design, our injection method should facilitate omics analyses and contribute to high performance clinical assays.
Perioperative Oral Pregabalin Reduces Chronic Pain After Total Knee Arthroplasty: A Prospective, Randomized, Controlled Trial (2009)

Perioperative pregabalin administration reduces the incidence of chronic neuropathic pain after TKA, with less opioid consumption and better range of motion during the first 30 days of rehabilitation. However, in the doses tested, it is associated with a higher risk of early postoperative sedation and confusion.
First human implant of the Alterra Adaptive PrestentTM: A new self‐expanding device designed to remodel the right ventricular outflow tract (2018)

Current balloon expandable transcatheter valves have limited applicability to patients with "native" right ventricular outflow tracts (RVOT), meaning those who have had previous surgery and are left with large, compliant, irregular RVOT. The Alterra Adaptive Prestent<sup>TM</sup> is a self-expanding, partially covered stent that was designed to internally reconfigure these types of RVOT, making them suitable for implantation of a commercially available balloon expandable heart valve, the SAPIEN 3. Herein, we describe the first human implant of this device.
Prognostic impact of PD-L1 in oropharyngeal cancer after primary curative radiotherapy and relation to HPV and tobacco smoking (2020)

<b>Background:</b> Incidence of oropharyngeal squamous cell carcinoma (OPSCC) is rising rapidly in many western countries due to <i>Human papillomavirus</i> (HPV) and tobacco smoking, with a considerable overlap. Immunotherapy directed at the PD1/PD-L1 axis have shown promise in head and neck cancer and other cancer types. PD-L1 expression may indicate a poorer prognosis, and at the same time indicate a possible benefit of anti-PD-L1 immunotherapeutic agents. The primary aim of this study was to establish the prognostic effect of PD-L1 expression after primary curative radiotherapy alone.<b>Material and methods:</b> A cohort of 303 OPSCC patients treated with primary, curative intended radiotherapy was established. PD-L1 expression was evaluated by immunohistochemistry on formalin fixed, paraffin embedded tissue sections. PD-L1 positivity was defined as a Combined Positive Score (CPS) ≥1, indicating staining of either tumor cells, lymphocytes or macrophages.<b>Results:</b> Median follow-up was 5.3 years. With 199 deaths, there was no difference in overall survival between patients with PD-L1+ and PD-L1- tumors (adjusted hazard ratio [aHR] and 95% confidence interval [CI]: 1.0 [0.71-1.4]). Also, locoregional failure was similar between the two groups (aHR 1.1 [CI: 0.68 - 1.7]). Tumors were PD-L1+ in 76% of cases, significantly more among HPV p16+ tumors (82% vs. 70%, <i>p</i> = .01). Interestingly, higher prevalence of PD-L1+ expression was seen in HPV p16+ patients with <10 pack-years of tobacco-smoking (93%) compared to HPV p16+ smokers (76%) or HPV p16-negative patients (70%) (<i>p</i> = .003).<b>Conclusion:</b> PD-L1 expression had no prognostic significance in OPSCC patients treated with primary radiotherapy alone. A substantial proportion of OPSCC tumors show PD-L1 overexpression, especially in HPV p16+ tumors in patients with little or no smoking history.
Increasing Productivity of Microreactors for Fast Gas−Liquid Reactions: The Case of Direct Fluorination of Toluene (2008)

The throughput of a single-channel microreactor used for a fast gas−liquid reaction was increased by up to one order of magnitude relative to previously published results by simultaneously increasing the superficial gas and liquid velocities. Superficial gas and liquid velocities varied between 2.9−14.6 m/s and 0.012–0.061 m/s, respectively. The direct fluorination of toluene in acetonitrile, selected as a model reaction, was performed at room temperature in microchannels formed in a silicon substrate, coated with thermally grown silicon oxide and evaporated nickel, and capped by Pyrex. With faster velocities the toluene conversion increased from 63% to 76%, while the combined selectivity of ortho-, meta-, and para-fluorotoluene isomers remained constant at 26%. Operating at faster gas and liquid velocities appeared to enhance the contacting between the two phases, thus outweighing the reduced liquid residence time.
ChemInform Abstract: Reversed Chemoselectivity in Titanium‐Induced Coupling Reactions: Syntheses of Salvadoricine and Diazepam. (1993)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.