gebhart/patrepeval huggingface dataset:
Cationic peptides obtained by reversible disaggregation of antibacterial proteins of bovine seminal plasma (1987)

Abstract A series of experiments was conducted to investigate the aggregating and disaggregating properties of anti‐bacterial proteins. in seminal plasma. Little of the antibactenal activity of bovme seminal plasma diffused through dialysis membrane of retentivity 10 kDa at pH 7, but at pH 3 or with 0.1 mol/litre citrate at pH 7 most of the activity diffused through. Some of this activity passed at pH 3 through an ultrafilter of retentivity 2 kDa. Gel filtration chromatography of the ultrafiltrate showed some reaggregation of material in this ultrafiltrate at pH 3, and reaggregation of all of it at pH 7. A fast‐migrating cationic antibacterial peptide was found by gel electrophoresis of both the acid and citrate dialysis diffusates of semmal plasma. On gel electrophoresis the same pattern of five cationic bands was found in the diffusate from acid dialysis of seminal plasma using membranes of retentivity 3.5, 8, and 12 kDa. One extra band was found with the 12 kDa membrane. All the bands were antibacterial. Bands of different electrophoretic mobility gave peaks in the same position on gel filtration chromatography. Reelectrophoresis of individual bands after recovery and incubation indicated that the pattern of five bands could be formed from a single band. Only two cationic bands were seen on cellulose acetate electrophoresis of the diffusate. The isoelectric points of these bands, determined from their mobility of different pH values, were 11.3 and 12.0. It is possible that most of the antibacterial acti.vity in seminal plasma is in the form of vanous aggregates of the same small molecular weight components, but further work is required before this can be concluded.
Robot-aided motion planning for knee joint rehabilitation with two robot-manipulators (2013)

In this paper, we propose a simultaneous design method of motion and external force trajectories for knee joint rehabilitation based on the biomechanical analysis of the lower limb. In this method we assume to use two robots manipulators which provide forces and moments at shank and thigh. We developed a 7 degree of freedom musculoskeletal model of lower limb with 19 muscles. The valuation function of rehabilitation efficiency e has been maximized by Genetic Algorithm (GA) that refers to the musculoskeletal model and tunes motion trajectory of the robots and forces acting on the shank and thigh.
ChemInform Abstract: NUCLEOPHILIC SUBSTITUTIONS OF (Z)‐PERCHLORO‐1,3‐BUTADIENE‐1‐CARBONITRILE WITH SODIUM PHENOLATE AND SECONDARY ALIPHATIC AMINES (1982)

Abstract Die Titelverbindung (I) reagiert mit NaOPh unter nucleophiler Substitution von drei Chloratomen zu dem Triphenoxyderivat (II) und mit den sekundären Aminen (III) ebenfalls unter Substitution von drei Chloratomen zu den Triaminoderivaten (IV), die beim Behandeln mit H 2 SO 4 zu den Pyridonen (V) cyclisieren.
ChemInform Abstract: 2‐Benzopyrylium Salts. Part 40. Addition Reaction of Azomethines with 2‐Benzopyrylium Salts. A New Method for the Synthesis of Isoquinoline Systems. (1991)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Discovery of 2-aminopyrimidines as potent agonists for the bitter taste receptor TAS2R14 (2022)

The bitter taste receptor TAS2R14 is a G protein-coupled receptor that is found on the tongue as well as in the human airway smooth muscle and other extraoral tissues. Because its activation causes bronchodilatation, TAS2R14 is a potential target for the treatment of asthma or chronic obstructive pulmonary disease. Structural variations of flufenamic acid, a nonsteroidal anti-inflammatory drug, led us to 2-aminopyridines showing considerable efficacy and potency in an IP-One accumulation assay. In combination with a bioisosteric exchange of the carboxylic moiety by a tetrazole unit, a set of promising new TAS2R14 agonists was developed. The most potent ligand 28.1 (EC50 = 72 nM) revealed a six-fold higher potency than flufenamic acid and a maximum efficacy of 129%. Besides its unprecedented TAS2R14 activation, 28.1 revealed marked selectivity over a panel of 24 non-bitter taste human GPCRs.
Time Course of Recovery of Erectile Function After Radical Retropubic Prostatectomy: Does Anyone Recover After 2 Years? (2010)

There is continued improvement in EF beyond 2 years after BNS RP. Discussion of this prolonged time course of recovery may allow patients to have a more realistic expectation.
Binocular contrast perception of gratings, noise, and natural images (2021)

The visual system is tasked with combining the two eye’s views into a single percept of the world. To understand the principles that underlie this combination, dichoptic stimuli (stimuli that have different levels of luminance and/or contrast in the two eyes) can be used to probe how each eye’s input contributes to the ultimate binocular percept. For example, contrast perception of simple dichoptic stimuli, such as sine wave gratings, is well-predicted as a weighted combination of the two eyes inputs typically biased towards the eye seeing higher contrast. We aimed to examine contrast perception of more complex dichoptic stimuli to determine how higher-order visual properties may influence the binocular percept. Using a perceptual matching task in which participants adjusted one stimulus (which was non-dichoptic) to match the perceived contrast of another stimulus (which was dichoptic), we examined the perceived contrast of three classes of stimuli: gratings, 1/f noise, and natural image patches. In addition to finding the closest perceptual match, we also asked participants to report on whether and how the two stimuli differed in appearance. We found that: 1) Spatial variation in the stimuli modulated binocular contrast perception; however, the effect depended on the stimulus type. Specifically, spatial manipulations that affected the perceptual matches for gratings (i.e., increasing or decreasing the contrast of the surrounding area in either eye) did not have a systematic effect on the matches for the noise and natural images. 2) Across all stimulus types, however, spatial manipulations did affect the proportion of trials in which participants reported finding an “exact match”. These results suggest that spatial frequency interactions and higher-level inferences can modulate both the interocular weighting and the perceptual interpretation during binocular combination.
Measurement of expired carbon dioxide, oxygen and volume in conjunction with pretest probability estimation as a method to diagnose and exclude pulmonary venous thromboembolism (2006)

This preliminary work demonstrates that the end-tidal pCO(2)/pO(2) averaged from 30 s of breathing can produce clinically relevant likelihood ratios for the diagnosis and exclusion of PE.
Zinc Metallochaperones Reactivate Mutant p53 Using an ON/OFF Switch Mechanism: A New Paradigm in Cancer Therapeutics (2018)

<b>Purpose:</b> Zinc metallochaperones (ZMC) are a new class of anticancer drugs that reactivate zinc-deficient mutant p53 by raising and buffering intracellular zinc levels sufficiently to restore zinc binding. <i>In vitro</i> pharmacodynamics of ZMCs indicate that p53-mutant activity is ON by 4-6 hours and is OFF by 24. We sought to understand the mechanism of this regulation and to translate these findings preclinically. We further sought to innovate the formulation of ZMCs to improve efficacy.<b>Experimental Design:</b> We performed <i>in vitro</i> mechanistic studies to determine the role of cellular zinc homeostatic mechanisms in the transient pharmacodynamics of ZMCs. We conducted preclinical pharmacokinetic, pharmacodynamic, and efficacy studies using a genetically engineered murine pancreatic cancer model (KPC) to translate these mechanistic findings and investigate a novel ZMC formulation.<b>Results:</b><i>In vitro</i>, cellular zinc homeostatic mechanisms that restore zinc to its physiologic levels function as the OFF switch in ZMC pharmacodynamics. <i>In vivo</i> pharmacokinetic studies indicate that ZMCs have a short half-life (< 30 minutes), which is sufficient to significantly improve survival in mice expressing a zinc-deficient allele (p53<sup>R172H</sup>) while having no effect in mice expressing a non-zinc-deficient allele (p53<sup>R270H</sup>). We synthesized a novel formulation of the drug in complex with zinc and demonstrate this significantly improves survival over ZMC1.<b>Conclusions:</b> Cellular zinc homeostatic mechanisms function as an OFF switch in ZMC pharmacodynamics, indicating that a brief period of p53-mutant reactivation is sufficient for on-target efficacy. ZMCs synthesized in complex with zinc are an improved formulation. <i>Clin Cancer Res; 24(18); 4505-17. ©2018 AACR</i>.
The Ecstasy and Agony of Assay Interference Compounds (2017)

ADVERTISEMENT RETURN TO ISSUEEditorialNEXTThe Ecstasy and Agony of Assay Interference CompoundsCourtney Aldrich, Carolyn Bertozzi, Gunda I. Georg, Laura Kiessling, Craig Lindsley, Dennis Liotta, Kenneth M. Merz, Jr., Alanna Schepartz, and Shaomeng WangView Author Information ACS Infectious Diseases ACS Central Science Journal of Medicinal Chemistry ACS Chemical Biology ACS Chemical Neuroscience ACS Medicinal Chemistry Letters Journal of Chemical Information and Modeling Biochemistry Journal of Medicinal ChemistryCite this: ACS Cent. Sci. 2017, 3, 3, 143–147Publication Date (Web):February 28, 2017Publication History Published online28 February 2017Published inissue 22 March 2017https://doi.org/10.1021/acscentsci.7b00069Copyright © 2017 American Chemical SocietyRIGHTS & PERMISSIONSArticle Views7818Altmetric-Citations68LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (445 KB) Get e-AlertsSUBJECTS:Assays,Drug discovery,Inhibition,Molecules,Screening assays Get e-Alerts
160 Gb/s single-channel unrepeatered transmission over 200 km of non-zero dispersion shifted fiber (2002)

We report on 160 Gbit/s single channel unrepeatered transmission over the record length of 200 km non-zero dispersion shifted fiber using an electro-absorption modulator for simultaneous demultiplexing and clock recovery, and distributed Raman amplification.
Hydroxyl radical generation and oxidative stress in Carassius auratus exposed to glyphosate and its formulation (2013)

Oxidative stress parameters and some antioxidant defense systems in the liver of Carassius auratus exposed to glyphosate and its formulation (Roundup®) have been studied. Fish were exposed to glyphosate and its formulation at concentrations of 0.032, 0.16, 0.8, and 4.0 mg L−1, all calculated on glyphosate basis, for 11 days. Hydroxyl radical generation as determined by electron spin resonance spectroscopy of its spin-adduct with α-phenyl-t-butyl nitrone increased with the concentration of Roundup®. Superoxide dismutase activities were decreased relative to control by 21%–46% when exposed to glyphosate and 45%–52% when exposed to Roundup®, suggesting that the formulation is more toxic than glyphosate alone. Catalase showed no difference between both groups.
ChemInform Abstract: Synthesis of Macrobicyclic Polyethers with Nitrogen Atom Bridgeheads. (1991)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Role of Dot1L and H3K79 methylation in regulating somatic hypermutation of immunoglobulin genes (2021)

Somatic hypermutation (SHM) and class-switch recombination (CSR) of the immunoglobulin (Ig) genes allow B cells to make antibodies that protect us against a wide variety of pathogens. SHM is mediated by activation-induced deaminase (AID), occurs at a million times higher frequency than other mutations in the mammalian genome, and is largely restricted to the variable (V) and switch (S) regions of Ig genes. Using the Ramos human Burkitt's lymphoma cell line, we find that H3K79me2/3 and its methyltransferase Dot1L are more abundant on the V region than on the constant (C) region, which does not undergo mutation. In primary naïve mouse B cells examined ex vivo, the H3K79me2/3 modification appears constitutively in the donor Sμ and is inducible in the recipient Sγ1 upon CSR stimulation. Knockout and inhibition of Dot1L in Ramos cells significantly reduces V region mutation and the abundance of H3K79me2/3 on the V region and is associated with a decrease of polymerase II (Pol II) and its S2 phosphorylated form at the IgH locus. Knockout of Dot1L also decreases the abundance of BRD4 and CDK9 (a subunit of the P-TEFb complex) on the V region, and this is accompanied by decreased nascent transcripts throughout the IgH gene. Treatment with JQ1 (inhibitor of BRD4) or DRB (inhibitor of CDK9) decreases SHM and the abundance of Pol II S2P at the IgH locus. Since all these factors play a role in transcription elongation, our studies reinforce the idea that the chromatin context and dynamics of transcription are critical for SHM.
On-line learning algorithms for locally recurrent neural networks (1999)

This paper focuses on on-line learning procedures for locally recurrent neural networks with emphasis on multilayer perceptron (MLP) with infinite impulse response (IIR) synapses and its variations which include generalized output and activation feedback multilayer networks (MLN's). We propose a new gradient-based procedure called recursive backpropagation (RBP) whose on-line version, causal recursive backpropagation (CRBP), presents some advantages with respect to the other on-line training methods. The new CRBP algorithm includes as particular cases backpropagation (BP), temporal backpropagation (TBP), backpropagation for sequences (BPS), Back-Tsoi algorithm among others, thereby providing a unifying view on gradient calculation techniques for recurrent networks with local feedback. The only learning method that has been proposed for locally recurrent networks with no architectural restriction is the one by Back and Tsoi. The proposed algorithm has better stability and higher speed of convergence with respect to the Back-Tsoi algorithm, which is supported by the theoretical development and confirmed by simulations. The computational complexity of the CRBP is comparable with that of the Back-Tsoi algorithm, e.g., less that a factor of 1.5 for usual architectures and parameter settings. The superior performance of the new algorithm, however, easily justifies this small increase in computational burden. In addition, the general paradigms of truncated BPTT and RTRL are applied to networks with local feedback and compared with the new CRBP method. The simulations show that CRBP exhibits similar performances and the detailed analysis of complexity reveals that CRBP is much simpler and easier to implement, e.g., CRBP is local in space and in time while RTRL is not local in space.
Fast A/V Channel Switching on a Wireless Home Network (2005)

A method of enabling fast switching between multimedia streams on an 802.11g network is described. This technique is particularly useful for A/V home network applications where it is desirable to simulate the fast channel switching of conventional analog TV channels in order to emulate the behaviour of multichannel TV
Genome-wide functional analysis of human cell-cycle regulators (2006)

Human cells have evolved complex signaling networks to coordinate the cell cycle. A detailed understanding of the global regulation of this fundamental process requires comprehensive identification of the genes and pathways involved in the various stages of cell-cycle progression. To this end, we report a genome-wide analysis of the human cell cycle, cell size, and proliferation by targeting >95% of the protein-coding genes in the human genome using small interfering RNAs (siRNAs). Analysis of >2 million images, acquired by quantitative fluorescence microscopy, showed that depletion of 1,152 genes strongly affected cell-cycle progression. These genes clustered into eight distinct phenotypic categories based on phase of arrest, nuclear area, and nuclear morphology. Phase-specific networks were built by interrogating knowledge-based and physical interaction databases with identified genes. Genome-wide analysis of cell-cycle regulators revealed a number of kinase, phosphatase, and proteolytic proteins and also suggests that processes thought to regulate G(1)-S phase progression like receptor-mediated signaling, nutrient status, and translation also play important roles in the regulation of G(2)/M phase transition. Moreover, 15 genes that are integral to TNF/NF-kappaB signaling were found to regulate G(2)/M, a previously unanticipated role for this pathway. These analyses provide systems-level insight into both known and novel genes as well as pathways that regulate cell-cycle progression, a number of which may provide new therapeutic approaches for the treatment of cancer.
Ristocetin is for use in the platelet aggregation studies for the evaluation of platelet dysfunction or platelet activation like von Willebrand Syndrome, the quantitation of von Willebrand factor and the identification of Bernard-Soulier Syndrome. II. PRINCIPLE (2013)

The ristocetin, a concentration of 1.0-1.5 mg/ml, normal platelet aggregates in rich plasma or citrated whole blood through a mechanism in which the release of endogenous ADP plays only a small role. Ristocetin-induced platelet aggregation at concentrations of 1.2 mg/ml is absent or significantly lower in patients with von Willebrand's syndrome. At concentrations of 1.5 mg/ml there is a lower degree of abnormality. The majority of patients with von Willebrand's syndrome shows a negative response, as well as patients with Bernard-Soulier syndrome. Aggregation study in whole blood is based on to the evaluation of the electric resistance changes. Two electrodes while immersed in the sample are rapidly covered with platelets which, at the very first contact, appear in shape of monolayer. When the aggregating agent is added, additional platelets tend to attach the monolayer previously formed, determining an impedance increase between the two electrodes.
Catalytic Ring-Opening Copolymerization of Limonene Oxide and Phthalic Anhydride: Toward Partially Renewable Polyesters (2013)

Catalytic ring-opening copolymerization of limonene oxide with phthalic anhydride was performed applying metal t-Bu–salophen complexes (t-Bu–salophen)MX; M = Cr, X = Cl (1), M = Al, X = Cl (2), M = Co, X = OAc (3), M = Mn, X = Cl (4), t-Bu–salophen = N,N-bis(3,5-di-tert-butylsalicylidene)diimine. The chromium and aluminum catalysts performed best, and catalyst 1 was selected for further studies. Investigating the effect of different cocatalysts in the copolymerization of limonene oxide and phthalic anhydride revealed that the onium salt PPN+Cl– showed the best activity while phosphines and N-heterocyclic-based amines showed a somewhat lower activity. 1H NMR and MALDI-ToF-MS spectra of the copolymers formed confirmed the alternating microstructure. Applying various mono-, di-, and trifunctional chain transfer agents (CTA)s such as water, (poly)alcohols, acids, and diamines in all cases resulted in a decrease in molecular weight while the PDI remained low, characteristic for an immortal system. Catalyst 1 proved to be robust in the presence of these CTAs not showing any drop of catalytic activity, which allows the formation of polyesters with tunable molecular weights and narrow PDIs and varying numbers of functionalities.
Radioimmunoassay of Plasma Aldosterone and its Clinical Application (1973)

NIH抗アルドステロン抗体を用いた, 血漿アルドステロンのRadioimmunoassay法の検討を行い, 臨床応用の可能性を検討した.血漿アルドステロンはジクロルメタンで抽出, Bush B5による炉紙クロマトグラフィーで分離後Radioimmunoassayを行つた.本法による正常範囲は安静臥位2.3～8.5ng/dl, 立位8.0～12ng/dlであつた.一方原発性アルドステロン症13例では16～130ng/dlであり, 明らかな高値を示した.アルドステロン分泌率に対し, 血漿アルドステロン濃度測定の意義について考察を加えた.
Out of User Space Storage and RDMA (2006)

User space applications must invoke kernels calls in order to have the operating system handle file system and device driver processing for storage requests. There is a high overhead associated with such calls, including the processing of suspending, scheduling and dispatching threads, interrupts, cache misses, etc. The overhead contributes to wasted processor cycles because all work on the thread being switched is stopped until the task switch is complete. The problem is worse for applications with a high storage I/O to compute ratio. Alternatives to enable user space applications to pass storage requests directly to I/O adapters without run-time involvement from the operating system would eliminate this overhead
Apartments: Technology for Fall Prevention* (2012)

of falls. In this paper, we deploy a Doppler radar-based gait measurement system into the apartments of thirteen seniors. In scripted walks, we show the system measures gait velocity with a mean error of 14.5% compared to the time recorded by a clinician. With a calibration factor, the mean error is reduced to 10.5%. The radar is a promising sensing technology for gait velocity in a day-to-day senior living environment.
SET‐LRP of methyl methacrylate initiated with CCl4 in the presence and absence of air (2010)

Abstract Single electron transfer‐living radical polymerization (SET‐LRP) represents a robust and versatile method for the rapid synthesis of macromolecules with defined architecture. The synthesis of poly(methyl methacrylate) via SET‐LRP in dimethyl sulfoxide (DMSO) by using CCl 4 as initiator is demonstrated in this work. Resorting to a rather simple Cu(0)/Me 6 ‐TREN catalyst a method was established that allowed for the straightforward design of well‐defined poly(methyl methacrylate). The reactions were performed at various temperatures (25, 50, 60, and 80 °C) and complete monomer conversion could be achieved. The polymerizations obeyed first order kinetic, the molecular weights increased linearly with conversion and the polymers exhibited narrow molecular weight distributions all indicating the livingness of the process. By providing a small amount of hydrazine to the reaction mixture the polymerization could be conducted in presence of air omitting the need for any elaborated deoxygenation procedures. This methodology offers an elegant way to synthesize functionalized poly(methyl methacrylate) with perfect control over the polymerization process as well as molecular architecture. © 2010 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 48: 2243–2250, 2010