gebhart/patrepeval huggingface dataset:
Dual lysosomal-mitochondrial targeting by antihistamines to eradicate leukaemic cells (2019)

Dual targeting of lysosomes and mitochondria constitutes a new promising therapeutic approach for leukaemia treatment, supporting the further clinical development. FUND: This work was funded by the Fundación Mutua Madrileña (RMR), CaixaImpulse (RMR), the Spanish Ministry of Economy (RMR), the Josep Carreras International Leukaemia Foundation (RMR), l'Obra Social "La Caixa" (RMR), and Generalitat de Catalunya (IJC).
Rapid generation of Shigella flexneri GMMA displaying natural or new and cross-reactive O-Antigens (2022)

Generalized modules for membrane antigens (GMMA) are exosomes released from engineered Gram-negative bacteria and represent an attractive vaccine platform for the delivery of the O-Antigen (OAg), recognized as the key target for protective immunity against several pathogens such as Shigella. Shigella is a major cause of disease in Low- and Middle-Income countries and the development of a vaccine needs to deal with its large serotypic diversity. All S. flexneri serotypes, except serotype 6, share a conserved OAg backbone, corresponding to serotype Y. Here, a GMMA-producing S. flexneri scaffold strain displaying the OAg backbone was engineered with different OAg-modifying enzymes, either individually or in combinations. This strategy rapidly yielded GMMA displaying 12 natural serotypes and 16 novel serotypes expressing multiple epitopes combinations that do not occur in nature. Importantly, a candidate GMMA displaying a hybrid OAg elicited broadly cross-bactericidal antibodies against a large panel of S. flexneri serotypes.
Study of sintering processes in copper and nickel by positron annihilation (1990)

Abstract In the advanced stage of non‐isothermal sintering, vacancy clusters are present as prevailing type of defects. Their concentration considerably decreases at further temperature increase, and it can be concluded that thermally activated conversion of multiple vacancies into climbable dislocations takes place. Hence, in conformity with recent experiments, coincidence of intensive material transport ( \documentclass{article}\pagestyle{empty}\begin{document}$ \dot \varepsilon $\end{document} e‐maximum) and decrease of defect concentration (recovery) can be understood.
Thermophysiological aspects of wearable robotics: Challenges and opportunities (2022)

Technological advancements in the last two decades have enabled development of a variety of mechanically supporting wearable robots (i.e. exoskeletons) that are transitioning to practice in medical and industrial settings. The feedback from industry and recent controlled studies is highlighting thermal discomfort as a major reason for the disuse of the devices and a substantial barrier to their long-term adoption. Furthermore, a brief overview of the devices and their intended applications reveals that many of the potential users are likely to face thermal comfort issues because of either high exertion or medically related high heat sensitivity. The aim of this review is to discuss these emerging thermal challenges and opportunities surrounding wearable robots. This review discusses mechanisms, potential solutions, and a platform for systematically measuring heat transfer inhibition caused by wearing of an exoskeleton. Lastly, the potential for substantial metabolic rate reduction provided by exoskeletons to reduce worker thermal strain in warm-to-hot conditions is also considered.
Enzyme Linked Immunosorbent Assay Using Alkaline Phosphatase Conjugated with Streptococcal Protein G (1988)

Protein G, an IgG-binding protein, purified from the surface of group G streptococci, was coupled to alkaline phosphatase. The conjugate was used for detection of polyclonal goat and rabbit antibodies and monoclonal mouse IgG1, IgG2a and IgG2b in an enzyme-linked immunosorbent assay. A two-step coupling procedure was used, in which glutaraldehyde was allowed to react with the enzyme, excess glutaraldehyde was then removed by dialysis, and finally protein G added to the glutaraldehyde-activated and polymerized alkaline phosphatase. The activity and yield of the conjugates were then tested in an enzyme-linked immunosorbent assay. Coupling of 25 micrograms protein G to 5 mg alkaline phosphatase gave a conjugate which could be used for more than 10,000 determinations with maximal antibody binding giving an absorbance of 2.0. Under these conditions, there was no need for separation of the reactants before using the protein G-alkaline phosphatase complex.
0367 Novel 3D-Printed Custom Oral Appliance Therapy: A Pilot Study (2022)

Abstract Introduction Oral appliance (OA) therapy protrudes the jaw in a more anterior position during sleep to treat obstructive sleep apnea. OA therapy can be used in combination or as an alternative to positive airway pressure (PAP) therapy. Most custom oral devices are fabricated with expensive manufacturing equipment or individually hand-made increasing treatment costs that prevent access to care for patients. Previous studies report concerns of cost-effectiveness of OA therapy and surveys of sleep physicians with cost concerns leading to barriers in prescribing OA therapy. More recent technological advancements in additive technology have allowed low-cost, custom fabrication of digitally designed OA. To date there have been no clinical studies that the authors are aware of that have investigated the feasibility of offering 3D printed custom OA therapy. Methods A novel OA was designed specifically for 3D-printing with additional features to improve clinician efficiency, patient comfort, and patient safety. A review of 3D-printed materials was completed to ensure biocompatibility requirements prior to clinical testing. Approval from the Committee for Protection of Human Subjects of University of Texas Health Science Center at Houston was obtained. Five participants had novel OA digitally designed based on scans of upper and lower teeth in addition to a bite registration recorded using a George Gauge. Participants were surveyed based on comfort of device and safety monitoring. Results Participants found the novel OA generally comfortable. Comfort questions were presented on a scale of 1 – 10, where ten is the highest level of comfort. On average participants found that the novel OA had comfort levels of 9.0 ± 0.77 on gums, 8.2 ± 0.92 on teeth, and 9.8 ± 0.20 of the jaw joints. No major adverse events were reported and the most common minor adverse event reported was increased salivation. Conclusion This pilot study illustrates the clinical feasibility of a novel 3D printed OA. Future studies will further investigate patient outcomes and clinician efficiency during clinical implementation of this novel OA. Though the use of 3D-printing technologies OA therapy has the potential to be offered at lower cost and delivered to the patient in one visit representing a paradigm shift. Support (If Any)
A cyclopeptide and three oligomycin-class polyketides produced by an underexplored actinomycete of the genus Pseudosporangium (2020)

Aside from the well-studied conventional actinomycetes such as <i>Streptomyces</i>, the less investigated genera of actinomycetes also represent a promising source of natural products. Genome mining indicated that members of the underexplored genus <i>Pseudosporangium</i>, from which no secondary metabolites have been reported to date, may harbor the biosynthetic machinery for the formation of novel natural products. The strain RD062863, that is available at a public culture collection, was obtained and subjected to metabolite analysis, which resulted in the discovery of a novel cyclopeptide, pseudosporamide (<b>1</b>), along with three new oligomycin-class polyketides, pseudosporamicins A-C (<b>2</b>-<b>4</b>). The unusual structure of compound <b>1</b>, featured by a biaryl-bond bridging across a tripeptide scaffold, <i>N</i>-acetyl-ʟ-Tyr-ʟ-Pro-ʟ-Trp, was determined by a combination of spectroscopic analyses, chemical derivatization, ECD calculation, and DFT-based theoretical chemical shift calculation, revealing the presence of an (<i>S</i> <sub>a</sub>)-axial chirality around the biaryl bond. Compounds <b>2</b>-<b>4</b> lacked hydroxylation on the side chain of the spiroacetal rings, which showed clear contrast to other oligomycin congeners and related polyketides with ring-truncation or expansion. The new macrolides <b>2</b>-<b>4</b> displayed potent antimicrobial activity against the Gram-positive bacterium <i>Kocuria rhizohpila</i> and the plant pathogenic fungus <i>Glomerella cingulata</i>. All compounds showed moderate cytotoxicity against P388 murine leukemia cells with IC<sub>50</sub> values in the micromolar to submicromolar ranges. These results exemplified the validity of phylogeny-focused strain selection combined with biosynthetic gene-directed genome mining for the efficient discovery of new natural products.
Functional Analysis of Dynactin and Cytoplasmic Dynein in Slow Axonal Transport (1996)

The neuron moves protein and membrane from the cell body to the synapse and back via fast and slow axonal transport. Little is known about the mechanism of microtubule movement in slow axonal transport, although cytoplasmic dynein, the motor for retrograde fast axonal transport of membranous organelles, has been proposed to also slide microtubules down the axon. We previously showed that most of the cytoplasmic dynein moving in the anterograde direction in the axon is associated with the microfilaments and other proteins of the slow component b (SCb) transport complex. The dynactin complex binds dynein, and it has been suggested that dynactin also associates with microfilaments. We therefore examined the role of dynein and dynactin in slow axonal transport. We find that most of the dynactin is also transported in SCb, including dynactin, which contains the neuron-specific splice variant p135(Glued), which binds dynein but not microtubules. Furthermore, SCb dynein binds dynactin in vitro. SCb dynein, like dynein from brain, binds microtubules in an ATP-sensitive manner, whereas brain dynactin binds microtubules in a salt-dependent manner. Dynactin from SCb does not bind microtubules, indicating that the binding of dynactin to microtubules is regulated and suggesting that the role of SCb dynactin is to bind dynein, not microtubules. These data support a model in which dynactin links the cytoplasmic dynein to the SCb transport complex. Dynein then may interact transiently with microtubules to slide them down the axon at the slower rate of SCa.
FM and FSK response of tunable two-electrode DFB lasers and their performance with noncoherent detection (1989)

Two-electrode distributed-feedback (DFB) lasers show promise for combining high speed and frequency tunability for frequency-division-multiplexed-frequency-shift-keyed (FDM-FSK) networks. The FM and FSK responses of such lasers have been measured up to modulation frequencies of approximately 1 GHz. Using these lasers in a noncoherent detection system in which a fiber Fabry-Perot tunable optical filter converts an FSK signal into an amplitude-shift-keyed (ASK) format, a 10/sup -9/ BER was observed up to 1 Gb/s. Nonuniform FM response and consequent tone broadening of the optical FSK spectra can lead to system power penalties due to optical-filtering effects. Thus, for a given FM response, the behavior of these lasers in FSK optical systems can be projected.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Cross Coupling (2008)

ADVERTISEMENT RETURN TO ISSUEEditorialNEXTCross CouplingStephen L. BuchwaldView Author Information Massachusetts Institute of TechnologyCite this: Acc. Chem. Res. 2008, 41, 11, 1439Publication Date (Web):November 18, 2008Publication History Published online18 November 2008Published inissue 18 November 2008https://doi.org/10.1021/ar8001798Copyright © 2008 American Chemical SocietyRIGHTS & PERMISSIONSArticle Views13452Altmetric-Citations148LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (54 KB) Get e-AlertsSUBJECTS:Addition reactions,Cross coupling reaction,Inorganic compounds,Substitution reactions,Vinyl Get e-Alerts
Transition Period Clinical Trajectories for PD versus HD Starters (2018)

For nephrology practice CKD patients, HD and PD starters appear clinically comparable in the year before dialysis start although HD starters exhibit a more rapid pre-dialytic eGFR decline. Ideally, studies comparing incident HD and PD outcomes should also consider CKD eGFR trajectories. In the first dialysis year, divergence occurs in albumin, white blood cell count, sodium and hemoglobin trends, which may be partly treatment-related.
Low frequency operation of Modular Multilevel Converters with embedded battery cells for traction drives (2016)

Traditional Modular Multilevel Converters (MMCs) need a special care to be taken for starting and for operation at low motor speeds in traction drives. Indeed, the low-frequency current may cause a significant unbalance between the converter sub-module capacitor voltages and then disturb the output waveforms. MMCs with the battery cells integrated inside the sub-modules are an alternative configuration where the traditional battery management system of battery electric vehicles is replaced by the control of the converter, which individually balances all the cells. The paper evaluates the low frequency operation of the MMCs with embedded battery cells when used as traction drives for battery electric vehicles by means of numerical simulations obtained from Matlab/ Simulink and eexperimental results from a down-scaled prototype. Results confirm that MMCs with embedded battery cells do not present any operational issue in traction drives even at low speed.
Approximate fairness through differential dropping (2003)

Many researchers have argued that the Internet architecture would be more robust and more accommodating of heterogeneity if routers allocated bandwidth fairly. However, most of the mechanisms proposed to accomplish this, such as Fair Queueing [16, 6] and its many variants [2, 23, 15], involve complicated packet scheduling algorithms. These algorithms, while increasingly common in router designs, may not be inexpensively implementable at extremely high speeds; thus, finding more easily implementable variants of such algorithms may be of significant practical value. This paper proposes an algorithm called Approximate Fair Dropping (AFD), which bases its dropping decisions on the recent history of packet arrivals. AFD retains a simple forwarding path and requires an amount of additional state that is small compared to current packet buffers. Simulation results, which we describe here, suggest that the design provides a reasonable degree of fairness in a wide variety of operating conditions. The performance of our approach is aided by the fact that the vast majority of Internet flows are slow but the fast flows send the bulk of the bits. This allows a small sample of recent history to provide accurate rate estimates of the fast flows.
Investigation of narrow-band thermal emission from intersubband transitions in quantum wells (2015)

We investigate thermal emission from n-doped GaAs/AlGaAs quantum wells (QWs). Emission peaks with Lorentzian shapes (linewidth 11∼19 meV) that reflect transitions between the first and second conduction subbands are observed in the mid-infrared range. It is demonstrated that the emission characteristics can be tuned by modifying the QW parameters. The peak emissivity is increased from 0.3 to 0.9 by modifying the doping density, and the peak wavelength is tuned from 6 to 10 μm by changing the well width. The obtained results are useful for the design of narrow-band thermal emitters.
Improvements to diffraction coefficient for non-perfectly conducting wedges (2005)

Holm proposed a heuristic uniform theory of diffraction coefficient for nonperfectly conducting wedges as an extension to the heuristic one given by Luebbers. This work improves limitations of Holm's diffraction coefficient in the illumination region, where it is not accurate. The accuracy of the improved diffraction coefficient is very close to that of Maliuzhinets' solution. The improvements are valid for parallel and perpendicular polarizations.
Embedding virtual topologies in networked clouds (2011)

Embedding virtual topologies in physical network infrastructure has been an area of active research for the future Internet and network testbeds. Virtual network embedding is also useful for linking virtual compute clusters allocated from cloud providers. Using advanced networking technologies to interconnect distributed cloud sites is a promising way to provision on-demand large-scale virtualized networked systems for production and experimental purposes.
Development of Neutralization Breadth against Diverse HIV‐1 by Increasing Ab–Ag Interface on V2 (2022)

Understanding maturation pathways of broadly neutralizing antibodies (bnAbs) against HIV-1 can be highly informative for HIV-1 vaccine development. A lineage of J038 bnAbs is now obtained from a long-term SHIV-infected macaque. J038 neutralizes 54% of global circulating HIV-1 strains. Its binding induces a unique "up" conformation for one of the V2 loops in the trimeric envelope glycoprotein and is heavily dependent on glycan, which provides nearly half of the binding surface. Their unmutated common ancestor neutralizes the autologous virus. Continuous maturation enhances neutralization potency and breadth of J038 lineage antibodies via expanding antibody-Env contact areas surrounding the core region contacted by germline-encoded residues. Developmental details and recognition features of J038 lineage antibodies revealed here provide a new pathway for elicitation and maturation of V2-targeting bnAbs.
Supplementary Data from Expression of Epidermal Growth Factor Receptor (EGFR) and Activated EGFR Predict Poor Response to (Chemo)radiation and Survival in Cervical Cancer (2023)

Supplementary Data from Expression of Epidermal Growth Factor Receptor (EGFR) and Activated EGFR Predict Poor Response to (Chemo)radiation and Survival in Cervical Cancer
Fluorinated Building Blocks. The Discovery of a Stable Difluoroallenyl Indium and the Synthesis of gem-Difluoroallenyl and -propargyl Synthons in Aqueous Media. (2000)

ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionNEXTORIGINAL ARTICLEThis notice is a correctionFluorinated Building Blocks. The Discovery of a Stable Difluoroallenyl Indium and the Synthesis of gem-Difluoroallenyl and -propargyl Synthons in Aqueous Media.ZhiGang Wang and Gerald B. HammondCite this: J. Org. Chem. 2000, 65, 24, 8406Publication Date (Web):November 3, 2000Publication History Published online3 November 2000Published inissue 1 December 2000https://pubs.acs.org/doi/10.1021/jo004010uhttps://doi.org/10.1021/jo004010ucorrectionACS PublicationsCopyright © 2000 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views339Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (11 KB) Get e-Alertsclose Get e-Alerts
Sorption of lysozyme by HEMA copolymer hydrogels (1996)

Sorption of lysozyme by 2-hydroxyethyl methacrylate (HEMA) copolymer hydrogels was studied as a function of pH and gel composition. Three types of HEMA gels were synthesized: neutral (HEMA), acidic (HEMA + acrylic acid), and basic (HEMA + dimethylaminoethyl methacrylate). Each gel was synthesized at four initial volume fractions to obtain different equilibrium swelling ratios and microstructures. Sorption as a function of time was measured for each gel at pH 7, 7.5, and 8. The rate of uptake by the acidic gels was more rapid than that by the neutral gels: To sorb 90% of the protein required only 1 h for the acidic gels but 15 days for neutral gels. Lysozyme did not adsorb or partition into the basic gels. The fractional approach to equilibrium was most rapid for the more swollen gels, and the effect of pH was small. The results reported here may be useful for rational design of new biomaterials where it is desirable to know the relative magnitude of the effects of composition, synthesis, and pH on protein sorption. © 1996 John Wiley & Sons, Inc.
A BJT-Based Temperature-to-Digital Converter With ±60 mK ( $3~\sigma$ ) Inaccuracy From −55 °C to +125 °C in 0.16-μm CMOS (2017)

This paper presents a precision CMOS temperature-to-digital converter (TDC), which senses the temperature-dependent base-emitter voltage of substrate PNPs. Measurements on 20 samples from one batch show that it achieves an inaccuracy of ±60 mK (3σ) from -55 °C to +125°C, after a single room-temperature trim. This state-of-the-art result is mainly due to the extensive use of dynamic error cancellation techniques to generate the PNP's collector currents, thus minimizing the spread in their base-emitter voltages, together with a digital PTAT trim to correct for the spread in the PNP's saturation currents. The effect of process variation on the TDC's inaccuracy was investigated by measuring 80 samples from three different batches. Using the same calibration parameters, they exhibit a maximum untrimmed inaccuracy of ±2°C (3σ) from -55°C to +125°C. This drops to ±100 mK (3σ) after a single point trim. The proposed TDC thus reduces calibration costs by obviating the need for batch-specific calibration parameters, which would otherwise require the multipoint calibration of several samples. The effect of the PNP's current gain β was also investigated with the help of a novel β-detection circuit. Implemented in 0.16-μm CMOS, the TDC occupies 0.16 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> and draws 4.6 μA from 1.5 to 2 V supply voltages. It achieves a resolution Figure of Merit of 7.8 pJ°C <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> , and a state-of-the-art supply sensitivity of 0.01°C/V.