gebhart/patrepeval huggingface dataset:
Data from Targeting the Oligomerization Domain of ETO Interferes with RUNX1/ETO Oncogenic Activity in t(8;21)-Positive Leukemic Cells (2023)

&lt;div&gt;Abstract&lt;p&gt;About 12% of all &lt;i&gt;de novo&lt;/i&gt; acute myeloid leukemias are characterized by the translocation t(8;21), which generates the oncogenic fusion protein RUNX1/ETO. RUNX1/ETO has a modular structure and contains several docking sites for heterologous proteins, including transcriptional corepressors like N-CoR, SMART, and mSIN3A. RUNX1/ETO is found in high molecular weight complexes, which are crucial for the block in myeloid differentiation observed in RUNX1/ETO–transformed cells. Essential for high molecular weight complex formation is the &lt;i&gt;nervy&lt;/i&gt; homology region 2 (NHR2) within ETO, which serves as interacting surface for oligomerization as well as association with members of the ETO protein family. Here, we show that the expression of a fusion peptide consisting of 128 amino acids (NC128), including the entire NHR2 domain of ETO, disrupts the stability of the RUNX1/ETO high molecular weight complexes, restores transcription of RUNX1/ETO target genes, and reverts the differentiation block induced by RUNX1/ETO in myeloid cells. In the presence of NC128, RUNX1/ETO–transformed cells lose their progenitor cell characteristics, are arrested in cell cycle progression, and undergo cell death. Our results indicate that selective interference with the oligomerization domain of ETO could provide a promising strategy to inhibit the oncogenic properties of the leukemia-associated fusion protein RUNX1/ETO. [Cancer Res 2007;67(5):2280–9]&lt;/p&gt;&lt;/div&gt;
The oncolytic peptide LTX-315 triggers necrotic cell death (2015)

The oncolytic peptide LTX-315 has been designed for killing human cancer cells and turned out to stimulate anti-cancer immune responses when locally injected into tumors established in immunocompetent mice. Here, we investigated the question whether LTX-315 induces apoptosis or necrosis. Transmission electron microscopy or morphometric analysis of chromatin-stained tumor cells revealed that LTX-315 failed to induce apoptotic nuclear condensation and rather induced a necrotic phenotype. Accordingly, LTX-315 failed to stimulate the activation of caspase-3, and inhibition of caspases by means of Z-VAD-fmk was unable to reduce cell killing by LTX-315. In addition, 2 prominent inhibitors of regulated necrosis (necroptosis), namely, necrostatin-1 and cycosporin A, failed to reduce LTX-315-induced cell death. In conclusion, it appears that LTX-315 triggers unregulated necrosis, which may contribute to its pro-inflammatory and pro-immune effects.
ChemInform Abstract: CARBON‐CARBON BOND FORMATION IN THE REACTION OF CALCIUM ATOMS WITH ETHERS (1980)

Abstract Die Umsetzung von Ca mit Dimethylether bei ‐196°C ergibt einen orange‐braunen festen Stoff, dessen Hydrolyse mit H 2 O oder D 2 O ein Kohlenwasserstoffgemisch ergibt, dessen Hauptbestandteile Acetylen (17% Gemischanteil), Ethylen (21%), Buten‐(l) (12%), cis‐ und trans‐Buten‐(2) (21%) und Hexan (15%) sind.
Eotaxin triggers eosinophil-selective chemotaxis and calcium flux via a distinct receptor and induces pulmonary eosinophilia in the presence of interleukin 5 in mice. (1996)

Mouse eotaxin is an eosinophil specific chemoattractant that has a markedly enhanced effect in vivo in the presence of another eosinophil selective cytokine IL-5, and utilizes a signal transduction receptor pathway that is distinct from that utilized by macrophage inflammatory protein-1 alpha. This data suggests that the development of tissue eosinophilia in vivo involves a two-step mechanism elicited by interleukin 5 and eotaxin.
Integrated circuit physically unclonable function (2023)

An integrated circuit (IC) based physically unclonable function (PUF) that comprises a common source amplifier for generating PUF output voltages, a unity gain, negative feedback operational amplifier for generating bias voltages, a voltage regulator and a bit exclusion circuit that excludes unstable PUF bits. Compensation circuitry built into the IC-PUF provides a high power supply rejection ratio and enables highly reliable operation of the IC-PUF across varying input voltages and operating temperatures. The IC-PUF generates a uniformly random output bit stream by taking advantage of process variations that are inherent to the fabrication of (metal-oxide semiconductor) MOS transistors.
ChemInform Abstract: Selective Removal of an N‐BOC Protecting Group in the Presence of a tert‐Butyl Ester and Other Acid‐Sensitive Groups (1994)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Pilot study of a novel serum mRNA gene panel for diagnosis of acute septic arthritis (2019)

In this pilot study, SMS showed a high level of diagnostic accuracy in predicting septic joints compared to other diagnostic biomarkers. This quick blood test could be an important tool for early, accurate identification of acute septic joints and need for emergent surgery, improving clinical care and healthcare spending.
Study and design of high efficiency switch mode GaN power amplifiers at L-band frequency (2009)

Activities have been carried out to determine the best electrical operating conditions of GaN HEMT that enable maximum power added efficiency at L-band for switch mode power amplifiers (class F, inverse class F and class E). Satellite radio navigation applications (Galileo) are targeted. Maximization of power added efficiency is of prime importance to save DC power consumption, reduce self heating effects and improve reliability of power amplifiers. At 50V drain bias, a maximum power added efficiency (PAE) of 72% and 40.3 dBm output power (Pout) are obtained using class-F operating conditions at 2dB gain compression while a 75% PAE and 41.0 dBm Pout are obtained using class E at 3dB gain compression.
Multi-GHz Pulse-Train X-Band Capability for Laser Compton X-Ray and Gamma-Ray Sources (2015)

A wide variety of light-source applications would benefit from increased average brightness, which generally corresponds to increasing average current in the driving accelerator. Presented is an accelerator architecture that is capable of producing hundreds of electron bunches, spaced as close together as every RF cycle, which provides the chance to increase current while maintaining beam quality. This system relies on an X-band photoinjector and a photoinjection drive laser that is driven by the same rf source to ensure synchronization, and an interaction laser system designed to match the duty cycle of the electron pulse train. Results of the photoinjector laser performance and initial experimental measurements of beam quality in accelerated bunch trains are presented, along with a discussion of the impact on the performance of tunable, narrow-bandwidth x-ray and gamma-ray beams based on Compton-scattering.
Phosphine Oxide Type Bipolar Host Material for High Quantum Efficiency in Thermally Activated Delayed Fluorescent Device (2014)

Highly efficient thermally activated delayed fluorescence devices were developed using a bipolar host material, 2,7-bis(diphenylphosphoryl)-9-phenyl-9H-carbazole (PPO27), derived from carbazole and diphenylphosphine oxide. The PPO27 host was effective for good charge balance and energy transfer from the PPO27 host to thermally activated delayed fluorescence dopant. The PPO27 device doped with (4s,6s)-2,4,5,6-tetra(9H-carbazol-9-yl)isophthalonitrile dopant could realize a high quantum efficiency of 24.2%.
<title>Bioreceptor-conducting polymer multilayer assemblies for biosensing</title> (1998)

This research focuses on the organized integration of biological receptors and polymers into thin film architectures for biosensing applications. Layer-by-layer electrostatic adsorption was used for the first time to form alternating protein-conducting polymer multilayers. The light-harvesting, phycobiliproteins and the enzyme, alkaline phosphatase were the bioreceptors investigated and sulfonated polystyrene, poly(diallyl dimethyl ammonium chloride) and a new enzymatically polymerized, water soluble, polyaniline were the polymer counterions used for deposition. Spectroscopic characterization was used to determine both multilayer formation and biosensing function of the final bioreceptor-polymer assemblies. These techniques have proven to be simple, chemically mild, and versatile and are expected to find application in the fabrication of ultrathin films for biosensors, opto- electronic devices and biomedical applications.
Electron Attachment Ionization (2016)

This document contains recommendations for terminology in mass spectrometry. Development of standard terms dates back to 1974 when the IUPAC Commission on Analytical Nomenclature issued recommendations on mass spectrometry terms and definitions. In 1978, the IUPAC Commission on Molecular Structure and Spectroscopy updated and extended the recommendations and made further recommendations regarding symbols, acronyms, and abbreviations. The IUPAC Physical Chemistry Division Commission on Molecular Structure and Spectroscopy’s Subcommittee on Mass Spectroscopy revised the recommended terms in 1991 and appended terms relating to vacuum technology. Some additional terms related to tandem mass spectrometry were added in 1993 and accelerator mass spectrometry in 1994. Owing to the rapid expansion of the field in the intervening years, particularly in mass spectrometry of biomolecules, a further revision of the recommendations has become necessary. This document contains a comprehensive revision of mass spectrometry terminology that represents the current consensus of the mass spectrometry community.
Cloning and expression of the Epstein--Barr virus-encoded dUTPase: patients with acute, reactivated or chronic virus infection develop antibodies against the enzyme (1996)

The gene encoding the Epstein-Barr virus (EBV)-specific dUTPase was amplified from virus DNA by PCR. The active enzyme was expressed in Escherichia coli and in insect cells as a non-fusion protein. The protein from E. coli specifically converted dUTP to dUMP and did not react with other dNTPs or NTPs. Preliminary experiments yielded a Km value of about 0.8 microM for dUTP. MAbs against the dUTPase reacted with a protein of approximately 31 kDa in 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-stimulated B cells harbouring either type 1 or type 2 EBV. The protein was found in untreated cells at low levels, whereas induction of the lytic replication cycle by TPA treatment or by providing the immediate early transactivator BZLF1 in trans resulted in increased expression. We demonstrated that the virus dUTPase isolated from EBV-infected cells is a phosphoprotein. The protein expressed in insect cells was used to test for the presence of specific antibodies in sera from normal, healthy carriers and from patients with various diseases. While the sera of EBV-negative individuals (0/3) or healthy carriers (0/33) did not contain detectable levels of antibodies, patients with mononucleosis (5/18), chronic EBV infection (2/7), EBV reactivation (7/20) and human immunodeficiency virus infection (5/24) showed elevated antibody titres against the enzyme. This indicated that the dUTPase is expressed during EBV replication and reactivation. The enzyme might therefore be a potential target for drug therapy under conditions of active DNA replication.
Tight Positive Regulation of Transgene Expression by a Single Adenovirus Vector Containing the rtTA and tTS Expression Cassettes in Separate Genome Regions (2003)

We previously developed single adenovirus (Ad) vectors that contained the components for a tetracycline-regulatable gene-expression system in the E1 and E3 deletion regions, and showed that the Ad vectors containing the tet-on system exhibit a much inferior regulation of transgene expression than those containing the tet-off system. In many cases, the tet-on system may be preferable because of its positive regulation of transgene expression. To this end, in the present study, by introducing the latest generation reverse tetracycline-responsive transcriptional activator (rtTA2s-M2 or rtTA2s-S2) and the tetracycline-controlled transcriptional silencer (tTS) into the original tet-on system, we constructed various modified Ad-mediated tet-on systems. Among them, the novel single Ad vector, which contained three heterologous gene-expression cassettes of the gene of interest, rtTA2s-S2, and tTS in the E1 deletion region, the E3 deletion region, and the region between E4 and 3'ITR, respectively, displayed vastly improved doxycycline-inducible gene expression in terms of low basal expression, high induced expression, and high responsiveness to doxycycline both in vitro and in vivo. These results also suggest that the low responsiveness to doxycycline may explain why the original tet-on system in the context of the Ad vector is not effective in vivo. This is the first report describing the cloning of three heterologous gene-expression cassettes into three separate regions of the E1/E3-deleted Ad genome. This improved Ad-mediated tet-on system might be useful for gene therapy and greatly facilitate the analyses of gene function.
FIR-median hybrid filters with excellent transient response in noisy conditions (1990)

Two classes of FIR-median hybrid (FMH) filters are introduced, which are called in-place growing FIR-median hybrid (IPG-FMH) and recursive in-place growing FIR-median hybrid (RIPG-FMH) filters. This new extended family of filters exhibits a very small bias error in the vicinity of an edge, an yet provides good noise attenuation. It is shown that the IPG-FMH filters can be expressed as weighted FMH (WFMH) filters.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Search for excited quarks of light and heavy flavor in γ + jet final states in proton–proton collisions at √s = 13 TeV (2018)

A search is presented for excited quarks of light and heavy flavor that decay to γ+jet final states. The analysis is based on data corresponding to an integrated luminosity of 35.9 fb⁻¹ collected by the CMS experiment in proton–proton collisions at √s=13TeV at the LHC. A signal would appear as a resonant contribution to the invariant mass spectrum of the γ+jet system, above the background expected from standard model processes. No resonant excess is found, and upper limits are set on the product of the excited quark cross section and its branching fraction as a function of its mass. These are the most stringent limits to date in the γ+jet final state, and exclude excited light quarks with masses below 5.5 TeV and excited b quarks with masses below 1.8 TeV, assuming standard model like coupling strengths.
Lethal myelofibrosis induced by Bmi1 -deficient hematopoietic cells unveils a tumor suppressor function of the polycomb group genes (2012)

Polycomb-group (PcG) proteins form the multiprotein polycomb repressive complexes (PRC) 1 and 2, and function as transcriptional repressors through histone modifications. They maintain the proliferative capacity of hematopoietic stem and progenitor cells by repressing the transcription of tumor suppressor genes, namely Ink4a and Arf, and thus have been characterized as oncogenes. However, the identification of inactivating mutations in the PcG gene, EZH2, unveiled a tumor suppressor function in myeloid malignancies, including primary myelofibrosis (PMF). Here, we show that loss of another PcG gene, Bmi1, causes pathological hematopoiesis similar to PMF. In a mouse model, loss of Bmi1 in Ink4a-Arf(-/-) hematopoietic cells induced abnormal megakaryocytopoiesis accompanied by marked extramedullary hematopoiesis, which eventually resulted in lethal myelofibrosis. Absence of Bmi1 caused derepression of a cohort of genes, including Hmga2, which is an oncogene overexpressed in PMF. Chromatin immunoprecipitation assays revealed that Bmi1 directly represses the transcription of Hmga2. Overexpression of Hmga2 in hematopoietic stem cells induced a myeloproliferative state with enhanced megakaryocytopoiesis in mice, implicating Hmga2 in the development of pathological hematopoiesis in the absence of Bmi1. Our findings provide the first genetic evidence of a tumor suppressor function of Bmi1 and uncover the role of PcG proteins in restricting growth by silencing oncogenes.
Somatic gene mutations expose cytoplasmic DNA to co-opt the cGAS/STING/NLRP3 axis in myelodysplastic syndromes (2022)

NLRP3 inflammasome and IFN-stimulated gene (ISG) induction are key biological drivers of ineffective hematopoiesis and inflammation in myelodysplastic syndromes (MDSs). Gene mutations involving mRNA splicing and epigenetic regulatory pathways induce inflammasome activation and myeloid lineage skewing in MDSs through undefined mechanisms. Using immortalized murine hematopoietic stem and progenitor cells harboring these somatic gene mutations and primary MDS BM specimens, we showed accumulation of unresolved R-loops and micronuclei with concurrent activation of the cytosolic sensor cyclic GMP-AMP synthase. Cyclic GMP-AMP synthase/stimulator of IFN genes (cGAS/STING) signaling caused ISG induction, NLRP3 inflammasome activation, and maturation of the effector protease caspase-1. Deregulation of RNA polymerase III drove cytosolic R-loop generation, which upon inhibition, extinguished ISG and inflammasome response. Mechanistically, caspase-1 degraded the master erythroid transcription factor, GATA binding protein 1, provoking anemia and myeloid lineage bias that was reversed by cGAS inhibition in vitro and in Tet2-/- hematopoietic stem and progenitor cell-transplanted mice. Together, these data identified a mechanism by which functionally distinct mutations converged upon the cGAS/STING/NLRP3 axis in MDS, directing ISG induction, pyroptosis, and myeloid lineage skewing.
ChemInform Abstract: Stereoselective Intramolecular 1,3‐Dipolar Cycloaddition of Chiral Chromium Tricarbonyl Complexed Nitrone. (1996)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Ultrasonication: Formation of Metal Nanoparticles Directly from Bulk Sources Using Ultrasound and Application to E‐Waste Upcycling (Small 17/2018) (2018)

In article number 1703615, Dale L. Huber and co-workers form nanoparticles directly from bulk metal using ultrasound. The method occurs in water and is applicable to a number of different metals and alloys. Any bulk metal form factor can be used, as long as there is a sufficiently large planar surface to induce asymmetric cavitation bubble collapse. The method is then applied to the recovery of gold from a cellular SIM card in the form of gold nanoparticles.
A vector handheld frequency-domain sensor for UXO identification (2009)

The GEM-3D<sup>+</sup> sensor developed by Geophex, Ltd. is a new incarnation of their widely known GEM-3. The sensor provides the analyst with all three vector components of the secondary magnetic field over a wide range of frequencies. The GEM-3D<sup>+</sup> features an innovative "beacon-based" positioning system that provides a full description of its location and orientation at every point without requiring any on-sensor hardware beyond an electronic compass. This enhances the usefulness of the instrument for dynamic surveying, This paper presents some methods and results related to UXO identification using the GEM-3D<sup>+</sup>. Our analyses exploit data provided by the sensor in both grid-based and dynamic measurements to characterize different objects, including metal spheres and actual UXO. For the data analysis we alternate between the dipole model and the more rigorous standardized excitation approach. We review some ill-conditioning issues encountered with the latter model and the different approaches that we use to overcome them. In applications, the availability of horizontal field components in the data allow us to identify UXO vs. non-UXO items while minimizing the nonnegligible effects of ground response.
Nano-positioning and tubulin conformation contribute to axonal transport regulation of mitochondria along microtubules (2022)

Correct spatiotemporal distribution of organelles and vesicles is crucial for healthy cell functioning and is regulated by intracellular transport mechanisms. Controlled transport of bulky mitochondria is especially important in polarized cells such as neurons that rely on these organelles to locally produce energy and buffer calcium. Mitochondrial transport requires and depends on microtubules that fill much of the available axonal space. How mitochondrial transport is affected by their position within the microtubule bundles is not known. Here, we found that anterograde transport, driven by kinesin motors, is susceptible to the molecular conformation of tubulin in neurons both in vitro and in vivo. Anterograde velocities negatively correlate with the density of elongated tubulin dimers like guanosine triphosphate (GTP)-tubulin. The impact of the tubulin conformation depends primarily on where a mitochondrion is positioned, either within or at the rim of microtubule bundle. Increasing elongated tubulin levels lowers the number of motile anterograde mitochondria within the microtubule bundle and increases anterograde transport speed at the microtubule bundle rim. We demonstrate that the increased kinesin velocity and density on microtubules consisting of elongated dimers add to the increased mitochondrial dynamics. Our work indicates that the molecular conformation of tubulin contributes to the regulation of mitochondrial motility and as such to the local distribution of mitochondria along axons.
Endometriosis Classification, Staging and Reporting Systems: A Review on the Road to a Universally Accepted Endometriosis Classification (2021)

Of the 22 endometriosis classification, staging and reporting systems identified in this historical overview, only a few have been evaluated for the purpose for which they were developed. The literature search was limited to PUBMED. Unpublished classification, staging or reporting systems, or those published in books were not considered. It can be concluded that there is no international agreement on how to describe endometriosis or how to classify it, and that most classification/staging systems show no or very little correlation with patient outcomes. This overview of existing systems is a first step in working towards a universally accepted endometriosis classification.
Glutamate Receptors: 7.3 AMPA and Kainate Receptors (2002)

This chapter contains sections titled: Introduction AMPA/Kainate and Pain Further Compounds
A High-speed Sorting System for Dried Prunes (1993)

A multi-camera, multiprocessor system was developed to sort prunes for surface defects in real-time. Three line-scan cameras were used to view the prune while in air. Each camera was connected to a subsystem computer to process the images simultaneously. Images were stored with a dual memory frame grabber circuit. An algorithm developed previously was modified to analyze and classify the images in less than 15 ms. Defective prunes were removed by a pneumatic system.