gebhart/patrepeval huggingface dataset:
Normal Human Keratinocytes Pretreated with Damaged mRNA Are Resistant to UVB Damage and Exhibit Increased DNA Repair (2004)

Treatment of normal human keratinocytes with UVC-irradiated rabbit globin mRNA 24 h before and after UVB exposure increased the survival of the human keratinocytes. We also observed that UVC-damaged mRNA reduced the formation of sunburn cells in skin models. We next tested the effects of UVC-damaged mRNA on cellular repair of DNA. DNA repair was evaluated using 2 assay methods. The first method used a damaged plasmid that is transfected back into the cell where it is repaired by the host cell repair mechanism. In these experiments, we observed that externally added UVC-damaged rabbit globin mRNA enhanced the repair of a plasmid transfected into the host keratinocyte cells. The second method used to determine the effects on DNA repair was direct immunostaining for thymidine-thymidine dimers (TT dimers) in histological sections of the skin models. Skin models were irradiated with UVB and then fixed immediately or after 24 h and stained for TT dimers. UVB irradiation immediately caused an increase in the number of stained keratinocytes in the skin. The number of stained cells decreased in skin fixed 24 h after UVB. This is due to repair of the TT dimers and their removal. Sections of skin models pretreated with UV-damaged mRNA exhibit greater removal of these TT dimers after 24 h. The above evidence suggests that damaged mRNA can trigger a host cell DNA repair pathway.
Staining Protocols for Safety Study of Wireless Fecobionics Device v1 (2022)

Protocols for Hematoxylin and Eosin staining and Immunofluorescence for two SPARC datasets: Safety Study of Wireless Fecobionics Device and Safety testing of predicate device for Fecobionics. The procedures were approved by Calmi2 Institutional Animal Care and Use Committee (IACUC) Mongrel dogs were randomly assigned into two groups: the Fecobionics device group, and the THD SensyProbe control group. All animals were anesthetized, the devices were transanally inserted into the rectum, and then inflated at 20 ml. Six minutes later the devices were removed either naturally or manually. The animals in each group were randomly assigned into two subgroups, acute and chronic. The dogs in the acute subgroups were euthanized immediately after the removal of the devices, whereas the dogs in the chronic groups were euthanized two weeks after the device removal. The tissue samples were harvested upon sacrifice.
Folic Acid and Homocysteine in Chronic Kidney Disease and Cardiovascular Disease Progression: Which Comes First (2017)

In the general population and in CKD patients, it remains a topic of discussion whether any beneficial effects of FA therapy are to be referred to its direct effect or to a reduction of Hhcy. While waiting for the results of confirmatory trials, it is reasonable to consider FA with or without methylcobalamin supplementation as appropriate adjunctive therapy in patients with CKD.
Dynamic nanophotonics and nonlinear optics with oxides and nitrides (2021)

Control over the permittivity and relaxation time in materials is important to gain control over the amplitude, phase and polarization of light. In this presentation, we report our work on controlling the static and dynamic optical properties of transition metal nitrides and transparent conducting oxides for a wide array of nanophotonic applications and nonlinear optical demonstrations. We find the carrier relaxation mechanism in titanium and zirconium nitride by pump-probe studies near the ENZ. We demonstrate high-harmonic generation in refractory TiN films. We show large changes in the epsilon-near-zero points in cadmium oxide via yttrium doping, for optically controlled, mid-IR reflectance modulation. In undoped zinc oxide, we demonstrate unity-order permittivity modulation by optical doping, with 20ps response time. With TiN-AZO Berreman-metasurfaces, we demonstrate simultaneous picosecond switching at telecom wavelengths, and nanosecond switching in the visible regime. Employing the ENZ effects in aluminum-doped zinc oxide, we demonstrate nonlinear optical phenomena spanning optical time-reversal to negative refraction. Our work will aid the development of dynamic devices for a wide array of dynamic optical applications.
Fine mapping of epitopes by intradomain Kd/Dd recombinants. (1987)

11 intradomain recombinants between H-2Kd and H-2Dd were produced using an original technique based on in vivo recombination in Escherichia coli. After transfection into mouse L cells, all these recombinants were expressed at high levels on the cell surface. The specificities of 77 mAbs were examined on these cell lines. mAbs could be organized in 12 groups. In each group, a small number of amino acids participating in the recognized epitope(s) were identified. In a few instances, noncontinuous epitopes comprising amino acids belonging to different domains of the antigen were found. The data thus obtained are compatible with those produced in previous exon-shuffling experiments, but permit a much more precise definition of recognized epitope(s).
Activation of Corticostriatal Circuitry Relieves Chronic Neuropathic Pain (2015)

Neural circuits that determine the perception and modulation of pain remain poorly understood. The prefrontal cortex (PFC) provides top-down control of sensory and affective processes. While animal and human imaging studies have shown that the PFC is involved in pain regulation, its exact role in pain states remains incompletely understood. A key output target for the PFC is the nucleus accumbens (NAc), an important component of the reward circuitry. Interestingly, recent human imaging studies suggest that the projection from the PFC to the NAc is altered in chronic pain. The function of this corticostriatal projection in pain states, however, is not known. Here we show that optogenetic activation of the PFC produces strong antinociceptive effects in a rat model (spared nerve injury model) of persistent neuropathic pain. PFC activation also reduces the affective symptoms of pain. Furthermore, we show that this pain-relieving function of the PFC is likely mediated by projections to the NAc. Thus, our results support a novel role for corticostriatal circuitry in pain regulation.
Challenges of lowering solar cell production costs by reducing silver content in back side pastes (2012)

For mass production of crystalline silicon solar cells, one can adopt two schools of thought when the goal is to improve the overall value of the finished product: increasing the overall efficiency and thus power output of the finished cells, or rather provide an equal power conversion efficiency while using less costly materials to construct the cell. While the choice of either option is based in each individual business ideology, we will discuss herein how the latter goal can be achieved by reducing the costly silver usage in back contact interconnect pastes. Standard back contact patterns can have a wide variety of designs depending on the exact technology (full bus bars, solder pads, etc.); however there is the necessary evil that a considerable quantity of silver must be deposited to provide an ideal solder joint. The recent rise in the cost of silver has sparked urgency to reduce its usage, and thus this work will demonstrate that it is possible to reduce the amount of silver in back contact pastes by as much as 30% or more relative to current commercially-available pastes. As is typical of engineering new products with such large generational leaps, many challenges must be overcome to ensure that the resulting low silver containing pastes will support the long module lifetimes promised by manufacturers. These testing procedures and methods of data analysis will be discussed.
Effect of physical properties estimation on equation-of-state predictions (1982)

The aim of this work was to document the influence of various heptanes-plus characterization schemes on equation-of-state predictions. Both the Peng-Robinson and a modified Redlich-Kwong cubic equation were used. Numerous characterization procedures were chosen from the literature. Six reservoir fluids with detailed heptanes-plus analysis were chosen to represent a broad range of fluid types. Pressure-volume-temperature measurements for these fluids were performed over a relatively wide range of conditions. One part of the study dealt with methods for estimating specific gravities and boiling points of petroleum fractions. Equation-of-state predictions were made using critical properties based on the estimated specific gravities and boiling points; these were compared with predictions made using critical properties based on measured specific gravities and boiling points. Another part dealt with various critical property correlations and their influence on equation-of-state predictions. A thorough review of commonly used correlations was presented. Four sets of correlations were compared. Results indicate that relatively small differences in critical properties and acentric factor can result in significant differences in equation-of-state predictions. Finally, a study was performed to document the influence of adjustments in heptanes-plus critical properties, equation-of-state constants (a and b) and binary interaction coefficients. Graphical presentations illustrate the influence of individual andmore » combined adjustments of these parameters.« less
Correlation of Diffraction MicroCT Images of Breast Tissue with Pathological Analysis (2006)

Identiflcation of tissue types in an imaged sample is limited using conventional X-ray imaging methods. The use of X-ray diffraction effects during imaging has been shown to have the potential to characterize the tissue type due to the fact that each tissue type produces its own unique diffraction signature. This paper shows a correlation between diffraction microCT images of excised breast tissue sections and histopathological analysis. A germanium detector based translate-rotate diffraction microCT system has been built to image the samples. Nineteen unfixed breast tissue samples were collected during mastectomy and tumour removal procedures and imaged. High resolution transmission microCT images were also recorded for comparison. Following imaging, the tissues were subjected to histopathological analysis. Reconstructing on various momentum transfer regions enables different tissue types to be identified in the diffraction images. Results showed a clear correlation between the measured X-ray diffraction images and the stained histopathological sections. X-ray diffraction spectra can be generated for the measured data sets for each of the individual tissue components within the sample. These can be compared with a database of tissue diffraction spectra for identification of tissue type.
ChemInform Abstract: One‐Pot Synthesis of Spiro Isochromane‐3,3′‐piperidines, ‐3,4′‐ piperidines, and ‐3,3′‐pyrrolidines. (1993)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Received-Signal-Strength Threshold Optimization Using Gaussian Processes (2017)

There is a big trend nowadays to use event-triggered proximity report for indoor positioning. This paper presents a generic received-signal-strength (RSS) threshold optimization framework for generating informative proximity reports. The proposed framework contains five main building blocks, namely the deployment information, RSS model, positioning metric selection, optimization process and management. Among others, we focus on Gaussian process regression (GPR)-based RSS models and positioning metric computation. The optimal RSS threshold is found through minimizing the best achievable localization root-mean-square-error formulated with the aid of fundamental lower bound analysis. Computational complexity is compared for different RSS models and different fundamental lower bounds. The resulting optimal RSS threshold enables enhanced performance of new fashioned low-cost and low-complex proximity report-based positioning algorithms. The proposed framework is validated with real measurements collected in an office area where bluetooth-low-energy (BLE) beacons are deployed.
Caleb E. Finch. Longevity, Senescence and the Genome. Chicago: University of Chicago Press, 1991, pp. 932. (1992)

RÉSUMÉ Ce livre de Caleb Finch est un survol bien documenté des divers aspects de la sénescence. Il trace un portrait de la sénescence allant du niveau moléculaire à l'interaction de l'organisme avec son environnement et se sert de douzaines de plantes et d'espèces animales comme exemple. Le livre est un condensé de données provenant de certaines d'articles de recherche. Des constantes du taux de mortalité, une bibliographie étendue et de multiples index sont inclus en annexe. Une des principales conclusions du livre est qu'il y a autant de sénescences que d'espèces, et que cela s'explique par les thèses évolutionnaires. Bien qu'à la base Longevity, Senescence and the Genome soit un livre sur la biologie comparée de la sénescence, il couvre assez le vieillissement de l'être humain pour intéresser les étudiants en gérontologie.
Electrostatic Actuators With Expanded Tuning Range Due to Biaxial Intrinsic Stress Gradients (2004)

A stress gradient was induced in two directions (through the plane of the beam and along its length) to produce a beam deflection of varying curvature. The stress gradient in the through-plane direction was produced by altering the conditions during electroplating and in the in-plane direction through lithographic patterning. The pull-down characteristics of four electrostatic actuator designs were analyzed. The in-plane stress gradient, along the length of the beam, significantly improved the tuning range compared with devices containing spatially uniform stress. This advance has greatly eased the instability problem of electrostatically actuated beams. The tuning range of a device with two hinges and a square front improved from less that 33% to 70%. Devices with two hinges and elliptical front improved to 45% tuning range and those with rectangular shape improved to 65% stability. This advance has been applied to actuators for RF tuners. Voltage cycling impacted only the initial cycling of the actuator.
Phosphorylation and disruption of intermediate filament proteins in oligodendrocyte precursor cultures treated with calyculin A (1993)

Treatment of primary cultures of oligodendrocyte precursors with calyculin A, a potent inhibitor of protein phosphatases 1 and 2A, caused the phosphorylation of two intermediate filament components, nestin and vimentin. Phosphoamino acid analysis demonstrated that phosphorylation took place mainly on serine and to a lesser extent on threonine residues. In addition, calyculin A treatment caused a shift in the distribution of the two proteins from the Triton-X-100 insoluble fraction to the detergent soluble fraction as demonstrated by immunoblotting. This redistribution, which was evident within 15 min after treatment and was nearly completed by 90 min, was accompanied by a disruption of the intermediate filament network. Thus, both nestin and vimentin retracted from the cytoplasmic processes to form a large perikaryal ring as shown by immunocytochemical analysis. Both morphological and biochemical changes were reversed 2-5 hr after removal of calyculin A from the culture medium.
CMS RPC commissioning of the existing detector during the long shutdown (2014)

February 1 (th) 2013 marked the end of the first period of running of the Large Hadron Collider (LHC) and the start of a two-year break from operation (LS1) aimed at consolidating both the accelerator as well as the detectors. By the end of LS1, the LHC is expected to provide collisions at 13 Tev. While, by 2020, the ultimate instantaneous luminosity is expected to be 10(34)/cm(2)/s. To prepare for this scenario, the Resistive Plate Chamber system at the CMS experiment is planning several detector maintainance and consolidation interventions. These include High Voltage and Low Voltage system reparations, gas leak identification and reparation, signal channel connectivity and functionality. Commissioning and upgrade plans for the existing CMS RPC system are presented here.
Crystal polymorphism in fragment-based lead discovery of ligands of the catalytic domain of UGGT, the glycoprotein folding quality control checkpoint (2022)

None of the current data processing pipelines for X-ray crystallography fragment-based lead discovery (FBLD) consults all the information available when deciding on the lattice and symmetry (i.e., the polymorph) of each soaked crystal. Often, X-ray crystallography FBLD pipelines either choose the polymorph based on cell volume and point-group symmetry of the X-ray diffraction data or leave polymorph attribution to manual intervention on the part of the user. Thus, when the FBLD crystals belong to more than one crystal polymorph, the discovery pipeline can be plagued by space group ambiguity, especially if the polymorphs at hand are variations of the same lattice and, therefore, difficult to tell apart from their morphology and/or their apparent crystal lattices and point groups. In the course of a fragment-based lead discovery effort aimed at finding ligands of the catalytic domain of UDP-glucose glycoprotein glucosyltransferase (UGGT), we encountered a mixture of trigonal crystals and pseudotrigonal triclinic crystals-with the two lattices closely related. In order to resolve that polymorphism ambiguity, we have written and described here a series of Unix shell scripts called <i>CoALLA</i> (<i>c</i>rystal p<i>o</i>lymorph <i>a</i>nd <i>l</i>igand <i>l</i>ikelihood-based <i>a</i>ssignment). The <i>CoALLA</i> scripts are written in Unix shell and use <i>autoPROC</i> for data processing, <i>CCP4-Dimple</i>/<i>REFMAC5</i> and <i>BUSTER</i> for refinement, and <i>RHOFIT</i> for ligand docking. The choice of the polymorph is effected by carrying out (in each of the known polymorphs) the tasks of diffraction data indexing, integration, scaling, and structural refinement. The most likely polymorph is then chosen as the one with the best structure refinement R<sub>free</sub> statistic. The <i>CoALLA</i> scripts further implement a likelihood-based ligand assignment strategy, starting with macromolecular refinement and automated water addition, followed by removal of the water molecules that appear to be fitting ligand density, and a final round of refinement after random perturbation of the refined macromolecular model, in order to obtain unbiased difference density maps for automated ligand placement. We illustrate the use of <i>CoALLA</i> to discriminate between H3 and P1 crystals used for an FBLD effort to find fragments binding to the catalytic domain of <i>Chaetomium thermophilum</i> UGGT.
Pulsed laser ablation synthesis and characterization of layered SrBi2Ta2O9 films and integration into capacitors for non-volatile memories (1997)

Abstract Pulsed laser ablation deposition (PLAD) was used to synthesize SrBi2Ta2O9 (SBT) layered ferroelectric thin films to integrate them into capacitors with top and bottom Pt electrodes produce by an ion beam sputter-deposition technique. SBT layers produced by laser ablation of stoichiometric and Bi-rich SBT targets exhibited marked different orientations. In addition, Pt/SBT/Pt capacitors fabricated with the distinctly oriented SBT layers exhibited substantial difference in the shape and parameters of polarization hysteresis loops, although they exhibited practically no polarization fatigue. The results presented in this paper indicate that the composition, microstructure and properties of SBT layers and SBT-based capacitors depend on the target composition and more studies are needed to understand these dependencies.
An analysis of multi-receiver, non-adaptive, slotted Aloha with capture for wireless communications in factories (2002)

An architecture for incorporating mobile data users into an existing MAP/TOP (Manufacturing Automation Protocol/Technical and Office Protocol) system is presented. The scheme attaches a small number of radio base stations to the TOP network through which the mobiles may access the system by executing a capture-based, multi-receiver extension of the traditional nonadaptive slotted Aloha protocol. A two-component capture model motivated by the factory channel is developed, and the multireceiver system's throughput performance is analyzed under two transmission models: base location independent and spatially correlated. It is seen that there is significant throughput degradation when base coverage areas overlap, indicating that this overlap should be minimized during system design. >
Low cost planar coil structure for inductive sensors to measure absolute angular position (2017)

There are many applications that need to sense angular displacement/position, such as automotive electronic steering system, throttle and brake pedal position, motors, robotic arm, etc. Contactless sensors are becoming increasingly popular in these kinds of applications due to their robustness, reliability and longevity. The paper proposes a new contactless variable reluctance (VR) resolver that can be built on a PCB as coil traces. The coil layout consists of an excitation coil and two sense coils laid on a single plane of a PCB. A circular metallic disk with a sector aperture alters the induced voltage in the sense coils. The induced voltage in the sense coils is a sine and cosine function of the angular position of disk. The absolute angular position can be measured with high resolution based on sense coil voltages. The design is validated using an ASIC to process the sensor signals and compute the angular position. The linearity of the coil design has been validated through experimental results.
Supplementary Movie 3 from Ovarian Cancer Spheroids Use Myosin-Generated Force to Clear the Mesothelium (2023)

&lt;p&gt;File Type: .mov; File Size: &lt;1 MB.&lt;/p&gt;
Evaluation of Particle Protection Capability of Extreme Ultraviolet Lithography Mask Carrier in Vacuum Transfer by Experiment and Calculation (2011)

In extreme ultraviolet lithography (EUVL), particle-free mask handling is a critical issue. We have studied particle protection capability of a dual pod, which consists of an outer pod and an inner pod that holds the mask. Our experiment and calculation demonstrated that a dual pod prevents particle addition in a quality area of 142×142 mm2 at the center of the mask, because particles entering the inner pod stopped near the edges. Our experiments showed 8 particle adders (≥46-nm polystyrene latex particle equivalent) for 1850-cycle transfer tests, and the dual pod satisfied the pre-production tool specification. Our measurement repeatability was 0.0015(σ)/cycle with a binominal distribution; however, we found that the accuracy was insufficient for an estimated specification of a high-volume machine. We discussed the necessary handling cycles and a method to improve repeatability by inspecting the area outside the quality area.