gebhart/patrepeval huggingface dataset:
Identification of Dihydromyricetin and Metabolites in Serum and Brain Associated with Acute Anti-Ethanol Intoxicating Effects in Mice (2021)

Dihydromyricetin is a natural bioactive flavonoid with unique GABA<sub>A</sub> receptor activity with a putative mechanism of action to reduce the intoxication effects of ethanol. Although dihydromyricetin's poor oral bioavailability limits clinical utility, the promise of this mechanism for the treatment of alcohol use disorder warrants further investigation into its specificity and druggable potential. These experiments investigated the bioavailability of dihydromyricetin in the brain and serum associated with acute anti-intoxicating effects in C57BL/6J mice. Dihydromyricetin (50 mg/kg IP) administered 0 or 15-min prior to ethanol (PO 5 g/kg) significantly reduced ethanol-induced loss of righting reflex. Total serum exposures (AUC0<sub>→</sub>24) of dihydromyricetin (PO 50 mg/kg) via oral (PO) administration were determined to be 2.5 µM × h (male) and 0.7 µM × h (female), while intraperitoneal (IP) administration led to 23.8-fold and 7.2- increases in AUC0<sub>→</sub>24 in male and female mice, respectively. Electrophysiology studies in α5β3γ2 GABA<sub>A</sub> receptors expressed in Xenopus oocytes suggest dihydromyricetin (10 µM) potentiates GABAergic activity (+43.2%), and the metabolite 4-O-methyl-dihydromyricetin (10 µM) negatively modulates GABAergic activity (-12.6%). Our results indicate that administration route and sex significantly impact DHM bioavailability in mice, which is limited by poor absorption and rapid clearance. This correlates with the observed short duration of DHM's anti-intoxicating properties and highlights the need for further investigation into mechanism of DHM's potential anti-intoxicating properties.
Retinoids as Critical Modulators of Immune Functions: New Therapeutic Perspectives for Old Compounds (2009)

Retinoids are vitamin A derivatives that critically regulate several physiological and pathological processes, including immune functions and cancer development. These biological response modifiers exert their pleiotropic effects through the interaction with nuclear receptors, defined as retinoic acid receptors (RARs) and retinoid X receptors (RXRs). These ligand-activated nuclear receptors induce the transcription of target genes by binding to responsive elements in the promoter regions. RARs and RXRs are also capable to interact with other nuclear receptors, thus expanding their spectrum of action on gene expression. Evidence has been accumulated indicating that retinoids may exert beneficial effects in both immune-mediated disorders and tumors. With regard to cancer, retinoids directly target neoplastic cells by inducing differentiation, inhibiting cell growth or promoting survival. However, the efficacy of these compounds in cancer treatment probably resides in their ability to modulate also the function of immune effectors. Vitamin A derivatives are currently used in the therapy of acute promyelocytic leukemia and of cutaneous T cell lymphomas, but they could be effective also on B-cell malignancies. Clinical trials are ongoing to test their efficacy in solid tumors. In this review, we give a broad depiction of how retinoids influence the function of immune effectors and affect growth and survival of hematological malignancies. This with the aim to better understand the clinical effects of retinoid-based therapies and provide the rationale to combine retinoids with other active compounds in new synergistic treatment strategies.
Antibiotika aus Basidiomyceten, XV [1]. 1-Hydroxy-2-nonin-4-on, ein antifungischer und cytotoxischer Metabolit aus Ischnoderma benzoinum (Wahl.) Karst. / Antibiotics from Basidiomycetes. XV [1]. 1-Hydroxy-2-nonyn-4-one, an Antifungal and Cytotoxic Metabolite from Ischnoderma benzoinum (Wahl.) Karst. (1982)

A new antibiotic, 1 -hydroxy-2-nonyn-4-one (1), has been isolated from submerged cultures of several strains of Ischnoderma benzoinum. The compound exhibits high inhibitory activity against yeasts and filamentous fungi at concentrations of 1-5 μg/ml. DNA, RNA, and protein syntheses in cells of the ascitic form of Ehrlich carcinoma are strongly inhibited by the same concentrations.
Novel Cavity Disinfectants Containing Quaternary Ammonium Monomer Dimethylaminododecyl Methacrylate (2016)

This study was set to assess the possible benefits of novel cavity disinfectants with 5% dimethylaminododecyl methacrylate (DMADDM); and compare the effectiveness of saliva microbial-aging method with water-aging in measuring the changing of resin-dentin bond strength. Three cavity disinfectants were tested: 0.2% Chlorhexidine (CHX); 5% DMADDM; and 5% DMADDM + 0.2% CHX. Microtensile bond strength (μTBS) test was performed after microbial-aging with saliva microbial or water aging for one month. Hydroxyproline (HYP), the production of collagen degradation, was measured spectrophotometrically. Additionally, the antibacterial effects of each reagent were evaluated. The 5% DMADDM exerted the least percentage of resin-dentin bond strength loss after one month microbial-aging (<i>p</i> < 0.05). There were no statistically significant differences of bond strength decrease after one month water aging among the tested groups (<i>p</i> > 0.05). Microbial-aging method yield more drop of bond strength than water aging in all groups except 5% DMADDM (<i>p</i> < 0.05). Meanwhile, 5% DMADDM had the same matrix metalloproteinases (MMPs) inhibitory effects as the other two agents (<i>p</i> > 0.05), but much stronger antibacterial capability than 0.2% CHX (<i>p</i> < 0.05). This indicated that a cavity disinfectant with 5% DMADDM is promising for improving the stability of resin-dentin bonds in appearance of saliva biofilm; and the saliva microbial-aging method is more promising for studying the durability of resin-dentin bonds than water aging.
Metallothionein‐III Prevents Glutamate and Nitric Oxide Neurotoxicity in Primary Cultures of Cerebellar Neurons (2000)

Metallothionein (MT)-III, a member of the MT family of metal-binding proteins, is mainly expressed in the CNS and is abundant in glutamatergic neurons. Results in genetically altered mice indicate that MT-III may play neuroprotective roles in the brain, but the mechanisms through which this protein functions have not been elucidated. The aim of this work was to assess whether MT-III is able to prevent glutamate neurotoxicity and to identify the step of the neurotoxic process interfered with by MT-III. Glutamate neurotoxicity in cerebellar neurons in culture is mediated by excessive activation of glutamate receptors, increased intracellular calcium, and increased nitric oxide. It is shown that MT-III prevented glutamate- and nitric oxide-induced neurotoxicity in a dose-dependent manner, with nearly complete protection at 0.3-1 microgram/ml. MT-III did not prevent the glutamate-induced rise of intracellular calcium level but reduced significantly the nitric oxide-induced formation of cyclic GMP. Circular dichroism analysis revealed that nitric oxide triggers the release of the metals coordinated to the cysteine residues of MT-III, indicative of the S(Cys)-nitrosylation of the protein. Therefore, the present results indicate that MT-III can quench pathological levels of nitric oxide, thus preventing glutamate and nitric oxide neurotoxicity.
Reference Values to Assess Hemodilution and Warn of Potential False-Negative Minimal Residual Disease Results in Myeloma (2021)

Our study supports the routine assessment of BM cellularity to evaluate hemodilution, since reduced BM-specific cell types as compared to reference values (either treatment-specific or from HA if the former are unavailable) could indicate hemodilution and a false-negative MRD result.
Synthesis and Characterization of Stable Monodisperse Silica Nanoparticle Sols for in Vitro Cytotoxicity Testing (2009)

For the investigation of the interaction of nanoparticles with biomolecules, cells, organs, and animal models there is a need for well-characterized nanoparticle suspensions. In this paper we report the preparation of monodisperse dense amorphous silica nanoparticles (SNP) suspended in physiological media that are sterile and sufficiently stable against aggregation. SNP sols with various particle sizes (2-335 nm) were prepared via base-catalyzed hydrolysis and polymerization of tetraethyl orthosilicate under sterile conditions using either ammonia (Stober process (1) ) or lysine catalyst (Lys-Sil process (2) ). The series was complemented with commercial silica sols (Ludox). Silica nanoparticle suspensions were purified by dialysis and dispersed without using any dispersing agent into cell culture media (Dulbecco's Modified Eagle's medium) containing antibiotics. Particle sizes were determined by dynamic light scattering. SNP morphology, surface area, and porosity were characterized using electron microscopy and nitrogen adsorption. The SNP sols in cell culture medium were stable for several days. The catalytic activity of the SNP in the conversion of hydrogen peroxide into hydroxyl radicals was investigated using electron paramagnetic resonance. The catalytic activity per square meter of exposed silica surface area was found to be independent of particle size and preparation method. Using this unique series of nanoparticle suspensions, the relationship between cytotoxicity and particle size was investigated using human endothelial and mouse monocyte-macrophage cells. The cytotoxicity of the SNP was strongly dependent on particle size and cell type. This unique methodology and the collection of well-characterized SNP will be useful for further in vitro studies exploring the physicochemical determinants of nanoparticle toxicity.
Voice Activity Detection Using Density Ratio Estimation of Speech and Noise (2013)

In this paper, we propose a robust voice activity detection (VAD) method that uses a density ratio model. For VAD under highly noisy environments, the likelihood ratio test (LRT) is effective. Conventional LRT constructs speech and noise models, calculates the likelihood of each model, and takes the ratio of those likelihoods to detect speech. Although some improved LRT have been proposed, in conventional LRT, it has not been taken into account that the likelihood ratio of speech and noise model is required, not the likelihood of each model. The proposed method directly estimates the likelihood ratio without calculating each likelihood using an density ratio model obtained in advance by density ratio estimation procedure. Moreover, there is the problem of determining thresholds, which are used for VAD and significantly affect its performance. We propose a method that automatically determines thresholds using discriminant analysis. The experiments show that the proposed method is more effective than conventional methods especially under non-stationary noisy environments.
Fifth-week immunogenicity and safety of anti-SARS-CoV-2 BNT162b2 vaccine in patients with multiple myeloma and myeloproliferative malignancies on active treatment: preliminary data from a single institution (2021)

BNT162b2 has demonstrated to be immunogenic at different extent among the cohorts. Response was 88% and robust in MPM patients. MM patients responded significantly less, particularly those on anti-CD38-based treatment. These latter patients should be advised to maintain masks and social distancing regardless of vaccination status, and their cohabiting family members need to be vaccinated in order to reduce the risk of contagion from the family. Additional boosters and titer monitoring could be considered. Trial registration Study was formally approved by the IRCCS Central Ethical Committee of Regione Lazio in January 2021 (Prot. N-1463/21).
Inhibition of proteases in Pseudomonas otitis media in chinchillas (1996)

The treatment of chronic suppurative otitis media caused by Pseudomonas aeruginosa remains a challenging problem. The virulence of Pseudomonas is related to its secretion of two matrix metalloproteinases, alkaline protease and elastase. This experiment examines the effects of a synthetic inhibitor of matrix metalloproteinases GM 6001, or N-(2(R)-2(hydroxyamido carbonylmethyl)-4-methylpentanoyl)-L-tryptophane methylamide), in a chinchilla Pseudomonas otitis media model. Thirty chinchillas underwent bilateral subtotal tympanic membrane perforations. Twenty-four chinchillas underwent bilateral middle ear inoculation with P. aeruginosa. Chinchillas were divided into four groups of six animals after the establishment of otitis media. Animals in one group were controls; the other three groups received either gentamicin, GM 6001, or gentamicin plus GM 6001 into the external auditory canal three times daily for 4 weeks. Clearance of Pseudomonas infection occurred in three ears of three animals, all in gentamicin groups, with or without GM 6001. Otorrhea (p = 0.0014) and external canal erythema (p = 0.025) were mild in the two gentamicin groups and moderate in the GM 6001 group when compared with bacterial controls. Animals in the GM 6001 group had the highest survival rate, less severe facial paralysis, and less vestibular toxicity than the gentamicin, gentamicin plus GM 6001, or control groups, although these differences were not statistically significant. This pilot study showed encouraging results for a role of ototopical protease inhibitors in the treatment of Pseudomonas otitis media.
Transverse imaging and simulation of dsDNA electrophoresis in microfabricated glass channels (2008)

We have observed the non-uniform distribution of DNA molecules during PAGE in a microfabricated system. Confocal laser scanning microscopy was used to visualize fluorescently labeled DNA during electrophoretic migration. The distribution of double-stranded DNA larger than 100 bp is observed to transition from a center-biased motion on the transverse plane 1 cm downstream from injection to an edge-biased motion 2 cm downstream. Although this distribution increased with increasing dsDNA size in a cross-linked gel, no similar distribution was found with the same dsDNA molecules in a linear polyacrylamide solution (6%). Simulations of DNA distribution in gels suggest that DNA distribution non-uniformities may be caused by biased electrophoretic migration resulting from motion in an inhomogeneous gel system.
Crystal Structures and Coordination Behavior of Aqua- and Cyano-Co(III) Tetradehydrocorrins in the Heme Pocket of Myoglobin (2016)

Myoglobins reconstituted with aqua- and cyano-Co(III) tetradehydrocorrins, rMb(Co(III)(OH2)(TDHC)) and rMb(Co(III)(CN)(TDHC)), respectively, were prepared and investigated as models of a cobalamin-dependent enzyme. The former protein was obtained by oxidation of rMb(Co(II)(TDHC)) with K3[Fe(CN)6]. The cyanide-coordinated Co(III) species in the latter protein was prepared by ligand exchange of rMb(Co(III)(OH2)(TDHC)) with exogenous cyanide upon addition of KCN. The X-ray crystallographic study reveals the hexacoordinated structures of rMb(Co(III)(OH)(TDHC)) and rMb(Co(III)(CN)(TDHC)) at 1.20 and 1.40 Å resolution, respectively. The (13)C NMR chemical shifts of the cyanide in rMb(Co(III)(CN)(TDHC)) were determined to be 108.6 and 110.6 ppm. IR measurements show that the cyanide of rMb(Co(III)(CN)(TDHC)) has a stretching frequency peak at 2151 cm(-1) which is higher than that of cyanocobalamin. The (13)C NMR and IR measurements indicate weaker coordination of the cyanide to Co(III)(TDHC) relative to cobalamin, a vitamin B12 derivative. Thus, the extent of π-back-donation from the cobalt ion to the cyanide ion is lower in rMb(Co(III)(CN)(TDHC)). Furthermore, the pK(1/2) values of rMb(Co(III)(OH2)(TDHC)) and rMb(Co(III)(CN)(TDHC)) were determined by a pH titration experiment to be 3.2 and 5.5, respectively, indicating that the cyanide ligation weakens the Co-N(His93) bond. Theoretical calculations also demonstrate that the axial ligand exchange from water to cyanide elongates the Co-N(axial) bond with a decrease in the bond dissociation energy. Taken together, the cyano-Co(III) tetradehydrocorrin in myoglobin is appropriate for investigation as a structural analogue of methylcobalamin, a key intermediate in methionine synthase reaction.
Pr[MOE‐DO3A], der Praseodymkomplex eines Tetraazacyclododecans: ein In‐vivo‐NMR‐Thermometer (1996)

Die Körpertemperatur im Innern eines lebenden Organismus zu bestimmen gelingt anhand der 1 H‐NMR‐chemischen Verschiebungen des paramagnetischen Praseodym‐Chelatkomplexes 1. Diese Verbindung ist sehr stabil und gut verträglich. In einer ersten In‐vivo‐Anwendung wurde die Temperatur in der Leber einer anästhetisierten Ratte mit einer Genauigkeit von besser als 0.5 K bestimmt. magnified image
Role of the HLA Class II (2007)

The paper highlights the role of different HLA class II molecules in hepatic and lymphoproliferative HCV-related disorders. HLA molecules have been reviewed, according to an in silico cluster classification, based on the sequence, the biochemical structure of the pockets, and the functional characteristics of the HLA II molecules. Thus, by reducing the complexity of HLA II polymorphism, characteristics that unite different HLA molecules with specific HCV-associated pathologies may be recognized with greater case. Results show that HLA clusters associated with better dlimination of the virus are protective against HCC development, while the same clusters are associated with a higher risk of developing cryoglobulinemic syndrome and the concomitant NHL. These data added further acknowledgements on pathogenetic mechanisms associated with HCV infection. Results also highlight differences of NHL occurring in HCV-positive subjects, with or without a concomitant type II autoimmune cryoglobulinemic syndrome, suggesting that cryoglobulinemic background associated with NHL should be considered in the evaluation of the effectiveness of new therapies in the course of HCV-associated NHLs.
Cochlear anatomy using micro computed tomography (μCT) imaging (2008)

A novel micro computed tomography (&mu;CT) image processing method was implemented to measure anatomical features of the gerbil and chinchilla cochleas, taking into account the bent modailosis axis. Measurements were made of the scala vestibule (SV) area, the scala tympani (SV) area, and the basilar membrane (BM) width using prepared cadaveric temporal bones. 3-D cochlear structures were obtained from the scanned images using a process described in this study. It was necessary to consider the sharp curvature of mododailosis axis near the basal region. The SV and ST areas were calculated from the &mu;CT reconstructions and compared with existing data obtained by Magnetic Resonance Microscopy (MRM), showing both qualitative and quantitative agreement. In addition to this, the width of the BM, which is the distance between the primary and secondary osseous spiral laminae, is calculated for the two animals and compared with previous data from the MRM method. For the gerbil cochlea, which does not have much cartilage in the osseous spiral lamina, the &mu;CT-based BM width measurements show good agreement with previous data. The chinchilla BM, which contains more cartilage in the osseous spiral lamina than the gerbil, shows a large difference in the BM widths between the &mu;CT and MRM methods. The SV area, ST area, and BM width measurements from this study can be used in building an anatomically based mathematical cochlear model.
Regenerated cellulose fibres (2001)

A brief history of regenerated cellulosic fibres Industrial cellulose The viscose process Lyocell The cuprammonium processes Fibres related to cellulose Other processes Physical structure and fibre properties Applications development Current and future market trends.
Relative Strain in Cu-Nb Composite Wound Wire (2016)

Journal Article Relative Strain in Cu-Nb Composite Wound Wire Get access R E Goddard, R E Goddard National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL USA Search for other works by this author on: Oxford Academic Google Scholar K H Han, K H Han National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL USA Search for other works by this author on: Oxford Academic Google Scholar D N Nguyen D N Nguyen Pulsed Field Facility (NHMFL), Los Alamos National Laboratory, Los Alamos, NM USA Search for other works by this author on: Oxford Academic Google Scholar Microscopy and Microanalysis, Volume 22, Issue S3, 1 July 2016, Pages 1992–1993, https://doi.org/10.1017/S1431927616010801 Published: 25 July 2016
Evaluating reward and risk for vertical selection (2012)

The aggregation of search results from heterogeneous verticals (news, videos, blogs, etc) has become an important consideration in search. When aiming to select suitable verticals, from which items are selected to be shown along with the standard "ten blue links", there exists the potential to both help (selecting relevant verticals) and harm (selecting irrelevant verticals) the existing result set.
Netted Multi-Function Radars Positioning and Modes Selection by Non-Holonomic Fast Marching Computation of Highest Threatening Trajectories & by CMA-ES Optimization (2019)

We aim to design a radar network which maximizes the detection probability of the worst threatening trajectory to reach a protected area. In game theory, we represent this problem as a non-cooperative zero-sum game: a first player chooses a setting for the network, and the other player chooses a trajectory from the admissible class of trajectories with full information over the network. The players' objective is respectively to maximize and minimize the path cost which is the detection probability integrated along the trajectory in the network. In comparison with previous works, we added ambiguity maps depending on the distance and the radial speed, which are functions of internal parameters of the radars that can be optimized: PRI (Pulse Repetition Interval) and frequency. We also take into account RCS (Radar Cross Section) and a complex geometry with masks deduced from DEM (Digital Elevation Map) and Earth curvature. The computation of optimal trajectories is performed with a specialized variant of the Fast-Marching algorithm, devoted to computing curves that globally minimize an energy featuring both a data driven term and a second order curvature penalizing term. The profile of the cost function with regard to the direction of movement is non-convex, which is significant only with a curvature penalization: we chose the Dubins model, in which the curvature radius is bounded. We illustrate results on different Use-Cases.
A Bioresorbable Magnetically Coupled System for Low‐Frequency Wireless Power Transfer (2019)

Abstract Bioresorbable electronic technologies form the basis for classes of biomedical devices that undergo complete physical and chemical dissolution after a predefined operational period, thereby eliminating the costs and risks associated with secondary surgical extraction. A continuing area of opportunity is in the development of strategies for power supply for these systems, where previous studies demonstrate some utility for biodegradable batteries, radio frequency harvesters, solar cells, and others. This paper introduces a type of bioresorbable system for wireless power transfer, in which a rotating magnet serves as the transmitter and a bioresorbable antenna as the remote receiver, with capabilities for operation at low frequencies (&lt;200 Hz). Systematic experimental and numerical studies demonstrate several unique advantages of this system, most significantly the elimination of impedance matching and electromagnetic radiation exposure presented with the types of radio frequency energy harvesters explored previously. These results add to the portfolio of power supply options in bioresorbable electronic implants.