gebhart/patrepeval huggingface dataset:
Modeling of lead-frame plastic CSPs for accurate prediction of their low-pass filter effects on RFICs (2001)

This paper presents a direct extraction method to construct the electrical models of lead-frame plastic chip scale packages for RF integrated circuits (RFICs) from the measured S-parameters. To evaluate the package effects on the reciprocal passive components, the insertion and return losses for an on-chip 50-/spl Omega/ microstrip line housed in a 32-pin bump chip carrier (BCC) package were analyzed based on the established package model. Excellent agreement with measurement has been found up to 15 GHz. When applied to the nonreciprocal active components, the gain variations for a heterojunction-bipolar-transistor array housed in an 8-pin BCC package have also been successfully predicted up to 22 GHz. Both cases have demonstrated that the package acts as a low-pass filter to cause a sharp cutoff for the RFIC components above a certain frequency.
Molecular identification of M. bovis BCG by Multiplex PCR (2016)

Mycobacterium bovis (M. bovis) BCG belongs to Mycobacterium tuberculosis complex (MTBC), highly relatedorganisms, which are 99.9 % similar at nucleotide level and phenotypically similar. Its differentiation from other membersof MTBC by conventional methods is laborious and time consuming. PCR provides a rapid alternative method fordifferentiation between members of MTBC. It depends on identification of region of difference (RD) which have beenlost during attenuation of M. bovis. Two different BCG strains (from two sources) were confirmed as a member of M.tuberculosis (MTB) complex (MTC) and as BCG strains by PCR using primers to a region of the 16S rRNA gene that isconserved in all mycobacteria and region of difference (RD1, RD4, RD9 and RD12) respectively. DNA of Mycobacteriumtuberculosis was used as a control to compare with BCG strains. The results showed that 16S rRNA gene was present inall tested strains, while the RD1, RD4, RD9 and RD12 were absent only in BCG strains
DVB‐S2 low density parity check codes with near Shannon limit performance (2004)

Abstract Low density parity check (LDPC) codes are chosen for the second generation digital video broadcasting (DVB) standard. In this paper, we review LDPC codes in general, present belief propagation decoding algorithm in simple terms, describe the standardized LDPC codes and show their performance. Copyright © 2004 John Wiley &amp; Sons, Ltd.
Chemoselective Hydrogenation Catalysts:  Pt on Mesostructured CeO2Nanoparticles Embedded within Ultrathin Layers of SiO2Binder (2004)

Pt on mesostructured CeO(2) nanoparticles embedded within ultrathin layers of highly structured SiO(2) binder shows highest activity reported with 80% selectivity for the chemoselective hydrogenation of crotonaldehyde. Characterization by transmission electron microscopy (TEM), CO adsorption, and X-ray photoelectron spectroscopy (XPS) show the presence of small Pt metal particles, preferentially located on CeO(2) (70%) together with the formation of Pt-CeO(2-x) sites at the interface between Pt and CeO(2) (4 nm) nanoparticles. These sites are able to polarize the carbonyl group and facilitate the selective hydrogenation of this with respect to the double bond.
Propiscin – a safe new anaesthetic for fish (2012)

We’ve built this digital research platform to provide academics everywhere with fast, stable and secure digital access to our library of over 110,000 scholarly books and 800,000 journal articles. We’ve tested the platform in close cooperation with leading academic institutions. If you have any questions or you notice something doesn’t quite work as it should, please visit our <a href="/publishing/faq?lang=en" class="text-primary">Help/FAQ</a> page and let us know.
In Vitro Stabilization of the Unoccupied Glucocorticoid Receptor by Adenosine 5'-Diphosphate* (1983)

The addition of ATP to rat liver cytosol slows the rate of heat inactivation of the unoccupied glucocorticoid receptor (25 C) and stimulates the rate of activation of the preformed glucocorticoid-receptor complex (15 C). Dose-response curves and kinetic studies show that ADP is as effective as ATP in stabilizing the unoccupied glucocorticoid receptor against heat inactivation. ATP can also be replaced by analogs with a hydrolysis-resistant alpha, beta-pyrophosphate linkage (5'-adenylyl methylenephosphonophosphate or 5'-adenylyl methylenephosphonate); however, the hydrolysis-resistant beta, gamma analog (5'-adenylyl methylenediphosophonate) is ineffective. The addition of creatine phosphate plus creatine kinase, a condition favoring ATP formation, stimulates the rate of inactivation of the unoccupied glucocorticoid receptor, and the effect is only partially overcome by ADP. A condition that favors ADP formation, the addition of creatine plus creatine kinase, has no effect on the rate of inactivation of the unoccupied receptor and does not decrease the protective effect afforded by ATP alone. Collectively, these results suggest that ATP stabilization of the steroid-binding site in vitro is due to ADP generated from the triphosphate by endogenous enzymes and is not due to phosphorylation or adenylation of the receptor by ATP. Unlike ATP stabilization of the steroid-binding site, the ATP-stimulated increase in the rate of activation of the preformed glucocorticoid-receptor complex (15 C) does not require hydrolysis of the beta, gamma-pyrophosphate bond. Dose-response curves show that both ATP and 5'-adenylyl methylenediphosophonate stimulate the rate of activation of the glucocorticoid-receptor complex. Quantitation of nucleotide levels in unfractionated rat liver cytosol by high performance liquid chromatography shows that the effective concentration of added ATP that produces an optimal response is within the physiological range reported for intact cells.
Low-temperature fabrication of Ll/sub 0/ ordered FePt alloy with perpendicular anisotropy by alternate monatomic layer deposition (2003)

Summary form only given. Ll/sub 0/-type ordered FePt alloy with large magnetic anisotropy has attracted much attention in recent years, since it is a potential material for extensive applications such as ultrahigh density recording media and bias magnets in monolithic microwave integrated circuit. A lot of studies were performed for FePt alloy films obtained by conventional deposition techniques followed by appropriate heat treatment. It is well known, however, the growth temperature and/or the post annealing temperature are required to be high (usually more than 500/spl deg/C) for the preparation of highly ordered Fe-Pt alloy films. For practical use, it is essential to reduce the growth temperature. In this paper, we propose that the alternate monatomic layer deposition should be one of powerful methods for preparing Ll/sub 0/-type ordered Fe-Pt alloy films at reduced temperature.
Combined blockade of complement C5 and TLR co-receptor CD14 synergistically inhibits pig-to-human corneal xenograft induced innate inflammatory responses (2021)

Inadequate supplies of donor corneas have evoked an escalating interest in corneal xenotransplantation. However, innate immune responses contribute significantly to the mechanism of xenograft rejection. We hypothesized that complement component C5 and TLR co-receptor CD14 inhibition would inhibit porcine cornea induced innate immune responses. Therefore, we measured cytokine release in human blood, induced by three forms of corneal xenografts with or without inhibitors. Native porcine cornea (NPC) induced interleukins (IL-1β, IL-2, IL-6, IL-8, IL-1ra), chemokines (MCP-1, MIP-1α, MIP-1β) and other cytokines (TNF, G-CSF, INF-γ, FGF-basic). Decellularized (DPC) and gamma-irradiated cornea (g-DPC) elevated the release of those cytokines. C5-blockade by eculizumab inhibited all the cytokines except G-CSF when induced by NPC. However, C5-blockade failed to reduce DPC and g-DPC induced cytokines. Blockade of CD14 inhibited DPC-induced cytokines except for IL-8, MCP-1, MIP-1α, and G-CSF, while it inhibited all of them when induced by g-DPC. Combined blockade of C5 and CD14 inhibited the maximum number of cytokines regardless of the xenograft type. Finally, by using the TLR4 specific inhibitor Eritoran, we showed that TLR4 activation was the basis for the CD14 effect. Thus, blockade of C5, when combined with TLR4 inhibition, may have therapeutic potential in pig-to-human corneal xenotransplantation. STATEMENT OF SIGNIFICANCE: Bio-engineered corneal xenografts are on the verge of becoming a viable alternative to allogenic human-donor-cornea, but the host's innate immune response is still a critical barrier for graft acceptance. By overruling this barrier, limited graft availability would no longer be an issue for treating corneal diseases. We showed that the xenograft induced inflammation is initiated by the complement system and toll-like receptor activation. Intriguingly, the inflammatory response was efficiently blocked by simultaneously targeting bottleneck molecules in the complement system (C5) and the TLR co-receptor CD14 with pharmaceutical inhibitors. We postulate that a combination of C5 and CD14 inhibition could have a great therapeutic potential to overcome the immunologic barrier in pig-to-human corneal xenotransplantation.
Fully-digital beamforming demonstration with Pi-Radio mmWave SDR platform (2020)

Pi-Radio's vision is to democratize wireless research by providing advanced mmWave Software Defined Radio (SDR) platforms to the community at plainly affordable price points. Pi-Radio's v1 SDR features a 4-channel fully-digital transceiver that operates in the 57-64 GHz band. Fully-digital (a.k.a. MIMO) transceiver architectures enable multiple simultaneous TX/RX beams, standing in stark contrast with phased arrays featuring analog beamformers that are capable of transmitting/receiving only one beam at a time. This opens up a whole set of research problems to work on, across virtually every layer of the protocol stack. In this demo, the team will: (1) prove the correct formation of different TX/RX beams by applying geometrically determined beamforming weights, and (2) prove the benefits of fully-digital beamforming by transmitting four independent streams of data with an OFDM-based physical layer.
Pilot Based Cross Phase Modulation Power Estimation (2017)

An inter-channel cross phase modulation power estimation method is proposed based on angular squeezing of polarization diversified pilot tones. Simulation and experiment verify the estimator successfully indicates the power of the cross phase modulation impairment.
Acylpeptide Hydrolase Inhibition as Targeted Strategy to Induce Proteasomal Down-Regulation (2011)

Acylpeptide hydrolase (APEH), one of the four members of the prolyl oligopeptidase class, catalyses the removal of N-acylated amino acids from acetylated peptides and it has been postulated to play a key role in protein degradation machinery. Disruption of protein turnover has been established as an effective strategy to down-regulate the ubiquitin-proteasome system (UPS) and as a promising approach in anticancer therapy.Here, we illustrate a new pathway modulating UPS and proteasome activity through inhibition of APEH. To find novel molecules able to down-regulate APEH activity, we screened a set of synthetic peptides, reproducing the reactive-site loop of a known archaeal inhibitor of APEH (SsCEI), and the conjugated linoleic acid (CLA) isomers. A 12-mer SsCEI peptide and the trans10-cis12 isomer of CLA, were identified as specific APEH inhibitors and their effects on cell-based assays were paralleled by a dose-dependent reduction of proteasome activity and the activation of the pro-apoptotic caspase cascade. Moreover, cell treatment with the individual compounds increased the cytoplasm levels of several classic hallmarks of proteasome inhibition, such as NFkappaB, p21, and misfolded or polyubiquitinylated proteins, and additive effects were observed in cells exposed to a combination of both inhibitors without any cytotoxicity. Remarkably, transfection of human bronchial epithelial cells with APEH siRNA, promoted a marked accumulation of a mutant of the cystic fibrosis transmembrane conductance regulator (CFTR), herein used as a model of misfolded protein typically degraded by UPS. Finally, molecular modeling studies, to gain insights into the APEH inhibition by the trans10-cis12 CLA isomer, were performed.Our study supports a previously unrecognized role of APEH as a negative effector of proteasome activity by an unknown mechanism and opens new perspectives for the development of strategies aimed at modulation of cancer progression.
Diastereoselective Synthesis and Configuration‐Dependent Activity of (3‐Substituted‐cycloalkyl)glycine Pyrrolidides and Thiazolidides as Dipeptidyl Peptidase IV Inhibitors. (2004)

Abstract For Abstract see ChemInform Abstract in Full Text.
SdAb heterodimer formation using leucine zippers (2013)

Single domain antibodies (sdAb) are variable domains cloned from camel, llama, or shark heavy chain only antibodies, and are among the smallest known naturally derived antigen binding fragments. SdAb derived from immunized llamas are able to bind antigens with high affinity, and most are capable of refolding after heat or chemical denaturation to bind antigen again. We hypothesized that the ability to produce heterodimeric sdAb would enable reagents with the robust characteristics of component sdAb, but with dramatically improved overall affinity through increased avidity. Previously we had constructed multimeric sdAb by genetically linking sdAb that bind non-overlapping epitopes on the toxin, ricin. In this work we explored a more flexible approach; the construction of multivalent binding reagents using multimerization domains. We expressed anti-ricin sdAb that recognize different epitopes on the toxin as fusions with differently charged leucine zippers. When the initially produced homodimers are mixed the leucine zipper domains will pair to produce heterodimers. We used fluorescence resonance energy transfer to confirm heterodimer formation. Surface plasmon resonance, circular dichroism, enzyme linked immunosorbent assays, and fluid array assays were used to characterize the multimer constructs, and evaluate their utility in toxin detection.
The hands-on orthopaedic robot "acrobot": early clinical trials of total knee replacement surgery (2003)

A "hands-on" robotic system for total knee replacement (TKR) surgery is presented. A computer tomography-based preoperative planning software is used to accurately plan the procedure. Intraoperatively, the surgeon guides a small special-purpose robot, called Acrobot, which is mounted on a gross positioning device. The Acrobot uses active constraint control, which constrains the motion to a predefined region, and thus allows the surgeon to safely cut the knee bones to fit a TKR prosthesis with high precision. A noninvasive anatomical registration method is described. The system has been successfully used in seven clinical trials with encouraging results.
Probability distributions for locations of calling animals, receivers, sound speeds, winds, and data from travel time differences (2006)

A new, nonlinear sequential Monte Carlo technique is used to estimate posterior probability distributions for the location of a calling animal, the locations of acoustic receivers, sound speeds, winds, and the differences in sonic travel time between pairs of receivers from measurements of those differences, while adopting realistic prior distributions of the variables. Other algorithms in the literature appear to be too inefficient to yield distributions for this large number of variables (up to 41) without recourse to a linear approximation. The new technique overcomes the computational inefficiency of other algorithms because it does not sequentially propagate the joint probability distribution of the variables between adjacent data. Instead, the lower and upper bounds of the distributions are propagated. The technique is applied to commonly encountered problems that were previously intractable, such as estimating how accurately sound speed and poorly known initial locations of receivers can be estimated from the differences in sonic travel time from calling animals, while explicitly modeling distributions of all the variables in the problem. In both cases, the new technique yields one or two orders of magnitude improvement compared with initial uncertainties. The technique is suitable for accurately estimating receiver locations from animal calls.
Photo‐embossed Surface Relief Structures with an Increased Aspect Ratios by Addition of a Reversible Addition‐Fragmentation Chain Transfer Agent (2008)

photo-embossed surface relief structures with significantly increased aspect ratios are prepared by addition of a reversible addition fragmentation chain transfer agent. The aspect ratios under inert atmosphere are successfully improved by a factor of almost 10. Moreover, aspect ratios obtained under ambient circumstances, preferred for industrial applications, are also significantly enhanced.
Distributed Feedback Laser Biosensor Noise Reduction (2013)

Label-free biosensors based upon detection of shifts in the emission wavelength, which occur when biological analytes are adsorbed on the surface of a solid-state laser, represent an important new class of sensors that can simultaneously provide high sensitivity and high resolution. We report on a signal processing approach that enables detection of lasing wavelength shifts as small as Δλ ~ 1.5 pm from a distributed feedback laser biosensor (DFBLB) fabricated upon a flexible plastic substrate and incorporated into standard-format microplates. Because the DFBLB can be optically pumped in a pulsed mode at a repetition rate that is substantially faster than the rate of biomolecular binding interactions, noise may be reduced through the ability to average multiple independent readings through integration of many lasing spectra within a single spectral integration period, and to subsequently use boxcar averaging to collapse multiple readings in a time sequence to a single time point. We have observe that the DFBLB occasionally produces nonclassical output spectra that can lead to an increase in wavelength shift noise, and therefore we implement a statistical metric to automatically determine whether an acquired spectrum should be discarded from analysis. The combined approaches are used to demonstrate the ability to detect a protein-protein interaction with an analyte concentration that would not otherwise be observable over background noise.
A first-principles study of compression twins in h.c.p. zirconium (1994)

Using fully self-consistent ab-initio techniques, we have determined values for (1122) and (1011) compression twin-boundary energies in h.c.p. zirconium. Experimentally, c-axis compression produces (1122) twins at temperatures below about 800 K; it has been suggested that this is due to the low energy of this type of twin. We find that the (10Tl) boundary has a much lower energy than the (1122) boundary, by a factor of four. Thus the ease of (1122) formation at low temperatures must be due to the other causes, such as a low barrier to twin nucleation. © 1994 Taylor &amp; Francis Group, LLC.
A broadband open-sleeve dipole antenna mounted above a tunable EBG AMC ground plane (2004)

A broadband open-sleeve dipole antenna is mounted above a tunable electromagnetic bandgap (EBG) surface resulting in a low-profile narrowband antenna system that is tunable over an octave bandwidth. The EBG surface is composed of an array of square metallic patches connected via tunable capacitors over a thin PEC backed dielectric substrate. The capacitors tune the resonance of the surface down in frequency resulting in a low-profile antenna system that can be tuned from 1.2 GHz to 2.3 GHz with a relative thickness of only /spl lambda//30 to /spl lambda//15 at the lower and upper frequency limits respectively.
African Swine Fever Virus and host response - transcriptome profiling of the Georgia 2007/1 strain and porcine macrophages (2021)

Abstract African swine fever virus (ASFV) has a major global economic impact. With a case fatality in domestic pigs approaching 100%, it currently presents the largest threat to animal farming. Although genomic differences between attenuated and highly virulent ASFV strains have been identified, the molecular determinants for virulence at the level of gene expression have remained opaque. Here we characterise the transcriptome of ASFV genotype II Georgia 2007/1 (GRG) during infection of the physiologically relevant host cells, porcine macrophages. In this study we applied Cap Analysis Gene Expression sequencing (CAGE-seq) to map the 5’ ends of viral mRNAs at 5 and 16 hours post-infection. A bioinformatics analysis of the sequence context surrounding the transcription start sites (TSSs) enabled us to characterise the global early and late promoter landscape of GRG. We compared transcriptome maps of the GRG isolate and the lab-attenuated BA71V strain that highlighted GRG virulent-specific transcripts belonging to multigene families, including two predicted MGF 100 genes I7L and I8L. In parallel, we monitored transcriptome changes in the infected host macrophage cells. Of the 9,384 macrophage genes studied, transcripts for 652 host genes were differentially regulated between 5 and 16 hours-post-infection compared with only 25 between uninfected cells and 5 hours post-infection. NF-kB activated genes and lysosome components like S100 were upregulated, and chemokines such as CCL24, CXCL2, CXCL5 and CXCL8 downregulated. Importance African swine fever virus (ASFV) causes haemorrhagic fever in domestic pigs with case fatality rates approaching 100%, and no approved vaccines or antivirals. The highly-virulent ASFV Georgia 2007/1 strain (GRG) was the first isolated when ASFV spread from Africa to the Caucasus region in 2007. Then spreading through Eastern Europe, and more recently across Asia. We used an RNA-based next generation sequencing technique called CAGE-seq to map the starts of viral genes across the GRG DNA genome. This has allowed us to investigate which viral genes are expressed during early or late stages of infection and how this is controlled, comparing their expression to the non-virulent ASFV-BA71V strain to identify key genes that play a role in virulence. In parallel we investigated how host cells respond to infection, which revealed how the ASFV suppresses components of the host immune response to ultimately win the arms race against its porcine host.
Conduction Channel Formation and Dissolution Due to Oxygen Thermophoresis/Diffusion in Hafnium Oxide Memristors (2016)

Transition-metal-oxide memristors, or resistive random-access memory (RRAM) switches, are under intense development for storage-class memory because of their favorable operating power, endurance, speed, and density. Their commercial deployment critically depends on predictive compact models based on understanding nanoscale physicochemical forces, which remains elusive and controversial owing to the difficulties in directly observing atomic motions during resistive switching, Here, using scanning transmission synchrotron X-ray spectromicroscopy to study in situ switching of hafnium oxide memristors, we directly observed the formation of a localized oxygen-deficiency-derived conductive channel surrounded by a low-conductivity ring of excess oxygen. Subsequent thermal annealing homogenized the segregated oxygen, resetting the cells toward their as-grown resistance state. We show that the formation and dissolution of the conduction channel are successfully modeled by radial thermophoresis and Fick diffusion of oxygen atoms driven by Joule heating. This confirmation and quantification of two opposing nanoscale radial forces that affect bipolar memristor switching are important components for any future physics-based compact model for the electronic switching of these devices.