gebhart/patrepeval huggingface dataset:
Systemic administration of mutant vesicular stomatitis virus (VSV) completely eradicates nasopharyngeal carcinoma (NPC) models (2006)

Proc Amer Assoc Cancer Res, Volume 47, 2006

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Introduction: Nasopharyngeal carcinoma (NPC) is a malignancy of the head and neck region with unique clinical and demographic features. Standard therapy for NPC achieves only modest survival rates, underscoring a need to develop novel therapies. Our group has previously investigated multiple novel molecular therapeutics including adenoviral vectors, and antisense oligonucleotide approaches. None has succeeded in complete eradication of established xenograft tumours. Hence, we were interested in examining the therapeutic potential of vesicular stomatitis viruses (VSV), which preferentially replicate in malignant cells, presumably due to defects in their interferon (IFN) signaling. Materials and Methods: Two EBV-positive human NPC models were utilized: the C666-1, and the C17 tumours. The MTS assay was utilized to assess viability in vitro ; apoptosis was measured using flow-based techniques including fraction of cells in the sub-G0/G1 phase, and caspase-3 activation. In vivo experiments were conducted using nu/nu mice, which are permissive to VSV infection. The VSV-delta51-GFP is a mutant VSV carrying the GFP reporter gene; control VSV was inactivated by UV radiation (30 min in laminar flow hood). Results: Our data demonstrated that VSV-delta51-GFP (MOI = 0.00001 to 10.0) was cytotoxic to C666-1 cells in a time and dose-dependent manner (75.4 to 87.2 killing at 72 hrs, respectively). Control VSV demonstrated no cytotoxicity. VSVdelta51 infection of C666-1 cells at MOI=1.0 induced apoptosis in > 6.2% of infected cells at 24 hrs and reached >10.8 % at 72 hrs. C666-1 cells (5x10^6) infected ex vivo with VSVdelta51-GFP (MOI=5.0) and then implanted into nu/nu mice demonstrated no tumour formation when injected subcutaneously, followed for 58 days, while C666-1 cells infected with UV-irradiated control virus formed tumors as early as day 27 post injection. Therapeutic efficacy of VSVdelta51-GFP was evaluated in both the C666-1 and C17 models. Four intravenous injections of VSVdelta51-GFP (5x1^8 pfu x 4) were able to completely eradicate established tumours followed for 31 days. No adverse effects were observed in mice treated with VSV; control VSV demonstrated no in vivo effect. Conclusion: These data demonstrate that VSV is a potentially promising novel oncolytic viral therapeutic modality for NPC. We are currently investigating the therapeutic benefit of combining VSV with RT and chemotherapy, and anticipate application to human patients in the near future if the combinatorial strategy demonstrates such impressive efficacy. Our finding may also be applicable to other human cancers.
Fundamental Limitations of SiC PVT Growth Reactors with Cylindrical Heaters (2006)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.
High-Throughput Screening of Formulations to Optimize the Thermal Stability of a Therapeutic Monoclonal Antibody (2014)

Monoclonal antibodies (mAbs) are an important class of biotherapeutics. Successful development of a mAb depends not only on its biological activity but also on its physicochemical properties, such as homogeneity and stability. mAb stability is affected by its formulation. Among the many techniques used to study the stability of mAbs, differential scanning fluorimetry (DSF) offers both excellent throughput and minimal material consumption. DSF measures the temperature of the protein unfolding transition (Tm) based on the change in fluorescence intensity of the environmentally sensitive dye SYPRO Orange. With DSF adapted to a 96-well plate format, we have shown that low-pH or high-salt concentrations decrease the thermal stability of mAb1, whereas some excipients, such as sucrose, polysorbate 80, and sodium phosphate, increase its stability. The basal fluorescence of SYPRO Orange was enhanced by the presence of detergents, limiting the use of this approach to diluted detergent solutions. Throughput of DSF can be increased further with the use of a 384-well plate. DSF thermograms are in good agreement with the melting profiles obtained by differential scanning calorimetry (DSC). The Tms determined by DSF and DSC were well correlated, with the former being on average lower by 3 °C.
Determination of muon momentum in the MicroBooNE LArTPC using an improved model of multiple Coulomb scattering (2017)

We discuss a technique for measuring a charged particle's momentum by means of multiple Coulomb scattering (MCS) in the MicroBooNE liquid argon time projection chamber (LArTPC). This method does not require the full particle ionization track to be contained inside of the detector volume as other track momentum reconstruction methods do (range-based momentum reconstruction and calorimetric momentum reconstruction). We motivate use of this technique, describe a tuning of the underlying phenomenological formula, quantify its performance on fully contained beam-neutrino-induced muon tracks both in simulation and in data, and quantify its performance on exiting muon tracks in simulation. We find agreement between data and simulation for contained tracks, with a small bias in the momentum reconstruction and with resolutions that vary as a function of track length, improving from about 10% for the shortest (one meter long) tracks to 5% for longer (several meter) tracks. For simulated exiting muons with at least one meter of track contained, we find a similarly small bias, and a resolution which is less than 15% for muons with momentum below 2 GeV/c.
Image enhancement by local information fusion with pre-processing and composed metric (2008)

An imaging enhancement technique capable of mitigating the effect of atmospheric turbulence on wide field-of-view images is presented. The method is based on the local information fusion from a set of short-exposure anisoplanatic images. Since anisoplanatism affects short-exposure images locally, the synthetic imaging algorithm uses local image quality information. Pre-processing of the source data and use of a composed image quality metric are considered. The synthetic imaging technique is tested on experimental data. The synthesized images show an improvement in image quality compared to the original set of images.
Major histocompatibility complex independent T cell receptor-antigen interaction: functional analysis using fluorescein derivatives. (1991)

We have isolated T cell receptor (TCR) cDNAs from fluorescein (FL)-specific human T cell clones (alpha FL beta FL), and transferred them to TCR beta- Jurkat cells in order to study direct FL-binding to the TCR. Using either FL-conjugated polymers (FL-polymer) or FL-substituted Sepharose beads, we are able to demonstrate the direct binding of antigen to the T cell surface, and the functional activation of the T cell transfectants. We present evidence against the involvement of major histocompatibility complex (MHC) molecules or antigen presentation in the interaction of FL with the alpha FL beta FL transfectants. Additionally, we have examined the effect of ring substitutions on the FL molecule as well as specific alterations of substituents attached to the 5' position, and we have found that all of them interfere with the functional recognition of the alpha FL beta FL TCR. These experiments demonstrate that TCRs like antibodies have intrinsic affinities for antigen, even without the involvement of MHC molecules.
Testing of a secondary concentrator integrated with a beam-down tower system under non-liquid cooling strategies (2018)

Three dimensional secondary concentrators are exposed to high radiation fluxes, part of which is absorbed by the reflecting material, leading to elevated wall temperatures which may cause reflectance degradation and mechanical distortion. Temperature monitoring and thermal management is required. Existing 3D secondary concentrators used in beam-up or beam-down tower plants use water-based convective cooling for which failures and leaks have been reported. The present work tested two alternative non-liquid-based cooling strategies: enhanced radiative cooling using a high-emissivity paint and forced-air convective cooling. The concentrator under no cooling enhancement reached temperatures above the acceptable limit, indicating the need for better cooling. Forced-convective cooling had the most noticeable effect and proved sufficient for the considered testing conditions. The impact of radiative cooling enhancement was lower in the considered incident flux conditions, as the initial temperature without enhancement was relatively low.
Late-Onset Leanness in Mice with Targeted Ablation of Melanin Concentrating Hormone Neurons (2006)

The observation that loss of orexin (hypocretin) neurons causes human narcolepsy raises the possibility that other acquired disorders might also result from loss of hypothalamic neurons. To test this possibility for body weight, mice with selective loss of melanin concentrating hormone (MCH) neurons were generated. MCH was chosen to test because induced mutations of the MCH gene in mice cause hypophagia and leanness. Mice with ablation of MCH neurons were generated using toxin (ataxin-3)-mediated ablation strategy. The mice appeared normal but, after 7 weeks, developed reduced body weight, body length, fat mass, lean mass, and leptin levels. Leanness was characterized by hypophagia and increased energy expenditure. To study the role of MCH neurons on obesity secondary to leptin deficiency, we generated mice deficient in both ob gene product (leptin) and MCH neurons. Absence of MCH neurons in ob/ob mice improved obesity, diabetes, and hepatic steatosis, suggesting that MCH neurons are important mediators of the response to leptin deficiency. These data show that loss of MCH neurons can lead to an acquired leanness. This has implications for the pathogenesis of acquired changes of body weight and might be considered in clinical settings characterized by substantial weight changes later in life.
Integrative molecular and clinical modeling of clinical outcomes to PD1 blockade in patients with metastatic melanoma (2019)

Immune-checkpoint blockade (ICB) has demonstrated efficacy in many tumor types, but predictors of responsiveness to anti-PD1 ICB are incompletely characterized. In this study, we analyzed a clinically annotated cohort of patients with melanoma (n = 144) treated with anti-PD1 ICB, with whole-exome and whole-transcriptome sequencing of pre-treatment tumors. We found that tumor mutational burden as a predictor of response was confounded by melanoma subtype, whereas multiple novel genomic and transcriptomic features predicted selective response, including features associated with MHC-I and MHC-II antigen presentation. Furthermore, previous anti-CTLA4 ICB exposure was associated with different predictors of response compared to tumors that were naive to ICB, suggesting selective immune effects of previous exposure to anti-CTLA4 ICB. Finally, we developed parsimonious models integrating clinical, genomic and transcriptomic features to predict intrinsic resistance to anti-PD1 ICB in individual tumors, with validation in smaller independent cohorts limited by the availability of comprehensive data. Broadly, we present a framework to discover predictive features and build models of ICB therapeutic response.
Synthesis of Si-Ge nanoscale structures via deposition of single-source (GeH3)4−nSiHn hydrides (2005)

Growth of nanoscale islands with distinct Si0.33Ge0.67, Si0.25Ge0.75, and Si0.20Ge0.80 compositions and uniform sizes is conducted on Si(100) via dehydrogenation of the single-source hydrides (H3Ge)2SiH2, (H3Ge)3SiH, and (H3Ge)4Si, respectively. High-spatial-resolution electron energy loss spectroscopy and Raman spectroscopy indicate homogeneous elemental concentrations within and among islands and confirm that their Si-Ge content is predetermined by the stoichiometry of the corresponding precursors. Z-contrast electron microscopy reveals distinct and perfectly epitaxial islands with atomically sharp interfaces grown via a smooth and continuous wetting layer ∼10Å thick. Cross-sectional electron microscopy shows monomodal distributions of islands with defect-free microstructures. Low-energy electron microscopy studies of the film formation reveal that the growth proceeds via the Stranski-Krastanov mode. Assemblies of coherent quantum dots with highly controlled Ge-rich concentrations produced by this method are desirable for their potentially useful optical properties.
Polyurethanes prepared from cyclocarbonated broccoli seed oil (PUcc): New biobased organic matrices for incorporation of phosphorescent metal nanocluster (2017)

ABSTRACT New biobased polyurethanes were synthesized from cyclocarbonated broccoli seed oil and different di‐ or triamines. The isocyanate‐free route to synthesize these polyurethanes was divided into three steps: the broccoli seed oil was first epoxydized, then carbonated with CO 2 at a pressure of 50 bar to convert oxiranes into cyclic carbonates, and finally polyurethanes were prepared through the reaction of the cyclocarbonated oil with three different di‐ or triamines, that is, butylene diamine, m ‐xylene diamine, and bis (hexamethylene triamine). The chemical reactions were monitored by Fourier transform infrared spectroscopy, nuclear magnetic resonance, and electrospray ionisation mass spectrometry and mechanical properties of thus‐prepared polyurethanes were determined by dynamic mechanical analysis and tensile characterization. An UV‐responsive material was finally prepared from butylene diamine based polyurethane integrating phosphorescent molybdenum nanoclusters. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017 , 134 , 45339.
Topical Analgesia for Endometrial Biopsy (2005)

I.
Electromechanical Dampers for Vibration Control of Structures and Rotors (2010)

The chapter describes the modelling and the experimental validation of different types of electromagnetic actuators used to damp the vibration in mechanical structures and machines. The first section describes the theoretical background based on an energetic approach. Section 2 is devoted to the description and analysis of possible configurations of electromagnetic actuators. The analysis is supported by the modelling of the different configurations. In section 3 the experimental validation is presented for four different types of damping devices: motional eddy current, transformer active based on sensor signal feedback, transformer active based on self sensing feedback and transformer semi-active. The analyses described in the present chapter lead to the following results: the vibration response of a motiona eddy current damper can be modeled as the series connection of a linear mechanical spring and a viscous damper. In general an eddy current machine behaves as a crank whose end is connected to two spring/damper series acting along orthogonal directions. The mechanical impedance is a band limited function affected by the pole of the electric circuit. The band limitation can be usefully exploited in vibration isolation systems addressed either to reduce the vibration at the fundamental resonant frequency and to
Global multiobjective optimization via estimation of distribution algorithm with biased initialization and crossover (2007)

Multiobjective optimization problems with many local Pareto fronts is a big challenge to evolutionary algorithms. In this paper, two operators, biased initialization and biased crossover, are proposed to improve the global search ability of RM-MEDA, a recently proposed multiobjective estimation of distribution algorithm. Biased initialization inserts several globally Pareto optimal solutions into the initial population; biased crossover combines the location information of some best solutions found so far and globally statistical information extracted from current population. Experiments have been conducted to study the effects of these two operators.
Veränderungen im Bereich des proximalen Aneurysmahalses nach endovaskulärer Ausschaltung infrarenaler Aortenaneurysmen (2000)

The risk of proximal endoleakage due to post-interventional dilatation of the aorta abdominalis seems to be low. Insufficient stent graft fixation requires a wide distance between the proximal end of the stent graft and the aneurysm. In the mid-term uncovered stent wires overlapping the renal arteries had no relevant influence on renal function.
Bifunctionalized resins. Applications to the synthesis of insect sex attractants (1978)

A 2% crosslinked divinylbenzene–styrene copolymer, incorporating benzoyl chloride groups, was used to monoprotect the symmetrical diol 1,10-decanediol. The corresponding monotrityl ether and cis-10-tetradecen-1-ol were prepared by this system, the latter by a Wittig reaction of a polymer-bound ylide with an aldehyde in the solution phase. A series of bifunctionalized resins containing proximally located trityl alcohol and benzoic acid groups was prepared in an attempt to prepare diol linked to the polymer via both a trityl ether and a benzoate linkage. It was established that these polymers bound 1,10-decanediol exclusively at one end only. Polymers, containing both trityl alcohol and benzoic acid groups in a random array or polymers containing proximally located trityl alcohol groups, gave mixtures of both monoprotected and doubly protected diol.
Saphir-Faser-Bragg-Gitterfür die Hochtemperatursensorik (2017)

Zusammenfassung Saphir-Faser-Bragg-Gitter (SFBG) sind neuartige Temperatursensoren, die mittels fs-Laserpulsen in einkristallinen Saphirfasern erzeugt werden, wobei durch Wellenlängenmultiplexen mehrere Gitter in eine Faser geschrieben werden können. SFBG wurden von Raumtemperatur (RT) bis zu 1900 ℃ getestet. Die Temperaturabhängigkeit ist parabolisch und steigt von ca. 23 p.m./K bei RT auf 35 p.m./K bei 1500 ℃. Durch eine angepasste Auswertung des Reflexionsspektrums wird eine Faser-Bragg-Wellenlänge bestimmt, wobei relative Temperaturänderungen von ± 1 K nachgewiesen werden können. SFBG werden durch elektromagnetische Felder nicht beeinflusst. Sie können sowohl in oxidierender als auch in reduzierender Atmosphäre eingesetzt werden. Allerdings müssen Saphirfasern durch Schutzrohre gegen Kontamination geschützt werden. Sowohl der kurzzeitige Einsatz bei 1900 ℃ als auch ein Einsatz über vier Wochen bei 1400 ℃ führte zu keiner nachweisbaren Drift oder Alterung der SFBG. SFBG sind eine mögliche Alternative zu Thermoelementen oder Pyrometern.
An unusual formaldehyde oxidizing system in Rhodococcus erythropolis grown on compounds containing methyl groups (1984)

During utilization of compounds containing methyl groups, the non-methylotrophic bacteria Rhodococcus erythropolis oxidized the methyl groups entirely to carbon dioxide. This oxidation was linked to the presence of an NAD-dependent formaldehyde dehydrogenase activity which was lost on dialysis. The activity could be restored by the addition of boiled extract but not by adding the known cofactors glutathione or tetrahydrofolate. A further dehydrogenase activity with formaldehyde as substrate was found in ethanolgrown cells. This activity could be differentiated from that in methyl group metabolizing cells.
Figure S2 from <i>BRCA1</i> Promoter Methylation Is Linked to Defective Homologous Recombination Repair and Elevated <i>miR-155</i> to Disrupt Myeloid Differentiation in Myeloid Malignancies (2023)

&lt;p&gt;Figure S2 shows the BRCA1 qMSP results and HR functional status of the patient samples at multiple time points.&lt;/p&gt;
Analysis of multiple listener equalization performance due to displacement effects (2003)

Traditionally, room response equalization is performed to enhance sound quality, by reducing the effects of reverberation, at a given listener. However, room responses vary with source and listener positions. Hence, in a multiple listener environment, equalization may be performed through averaging the room responses measured at multiple listener locations. However, the performance of averaging based equalization, at the listeners, may be affected when listener positions change or due to microphone-listener position mismatch (i.e., displacement effects). In this paper, we present a statistical approach to map variations in listener positions to equalization performance of spatial average methods. The analysis is done at frequencies above the Schroeder frequency where the direct and the reverberant sound fields are uncorrelated, and the results are presented in a 3-D plot to clearly show the changes in equalization performance (in dB’s) versus mismatch and frequencies for various listener configurations relative to a fixed loudspeaker source. The results indicate that, for the analyzed listener configurations, the zone of equalization depends on distance of microphones/listeners from the source and the frequencies in the sound. [Work supported by the U.S. Dept. of Army.]
Modeling and Control of Diesel Engines Equipped with a Two-Stage Turbo-System (2008)

&lt;div class="htmlview paragraph"&gt;The two-stage turbocharging technique is an effective way to improve performance and reduce emissions in diesel engines. In this paper, we consider a diesel engine equipped with an exhaust gas recirculation (EGR) valve and two turbochargers in series. The low pressure turbine is of fixed geometry and the high pressure turbine is a variable geometry turbine (VGT). The control objective is to regulate air-to-fuel (AFR), EGR exhaust fraction and the power ratio of the two turbines by coordinated manipulation of the EGR and VGT actuators. Unlike engines with a single turbocharger, in two-staged turbocharged engines, regulation of the power ratio of the turbines is also needed in order to adequately define the equilibrium point of the engine airpath.&lt;/div&gt; &lt;div class="htmlview paragraph"&gt;First, a mean value engine model (MVEM) is proposed to physically describe the air path dynamics. With rich excitation of the controls in the MVEM, we identify several linear models for different areas of the engine speed-torque envelope. Local Linear Quadratic Gaussian (LQG) controllers are designed, with each of them enabled within a specific operating area.&lt;/div&gt;
Virtual-freezing fluorescence imaging flow cytometry (2020)

ABSTRACT By virtue of the combined merits of flow cytometry and fluorescence microscopy, imaging flow cytometry (IFC) has become an established tool for cell analysis in diverse biomedical fields such as cancer biology, microbiology, immunology, hematology, and stem cell biology. However, the performance and utility of IFC are severely limited by the fundamental trade-off between throughput, sensitivity, and spatial resolution. For example, at high flow speed (i.e., high throughput), the integration time of the image sensor becomes short, resulting in reduced sensitivity or pixel resolution. Here we present an optomechanical imaging method that overcomes the trade-off by virtually “freezing” the motion of flowing cells on the image sensor to effectively achieve 1,000 times longer exposure time for microscopy-grade fluorescence image acquisition. Consequently, it enables high-throughput IFC of single cells at &gt;10,000 cells/s without sacrificing sensitivity and spatial resolution. The availability of numerous information-rich fluorescence cell images allows high-dimensional statistical analysis and accurate classification with deep learning, as evidenced by our demonstration of unique applications in hematology and microbiology.