gebhart/patrepeval huggingface dataset:
Analytical methodology for the determination of aflatoxins in peanut butter: comparison of high-performance thin-layer chromatographic, enzyme-linked immunosorbent assay and high-performance liquid chromatographic methods (1990)

High-performance thin-layer chromatography (HPTLC) was applied to the separation and quantification of aflatoxin in 300 jars of "crunchy" peanut butter. A critical evaluation of the proposed HPTLC method has been carried out by statistical comparisons with a commercially available enzyme-linked immunosorbent assay (ELISA) kit and a high-performance liquid chromatographic (HPLC) method. The statistical tests indicated that whilst the distributions of the data sets obtained with each method were similar, the HPLC method was found to be biased. Over-all results indicated that the HPTLC method gave more consistent data, relatively lower standard deviations and lower coefficients of variation. The ELISA kit was found to be less precise than the HPTLC and HPLC methods and prone to some loss of sensitivity caused by matrix interference.
Attempts at new synthesis of 5,11 ‐Dihydro‐6H‐pyrido[2,3‐b][1,4]benzodiazepin‐6‐one (1983)

Abstract Attempting some new approaches to 5,11‐dihyro‐6 H ‐pyrido[2,3‐ b ][1,4]benzodiazepin‐6‐one ( 6 ), compounds 8, 10 and 11 were prepared. Ring enlargement of 4 into 6 failed, as well as condensation of 10 and 11 into ID, which is the potential precursor of pirenzepin (11‐[2′‐(4″‐methylpiperazin‐1″‐yl)]acetyl derivative of 6 ) via an envisaged intramolecular Diels‐Alder reaction. Model compounds 5 and 13 were prepared and their behaviour in analogous reactions explained the failures of the intended transformations of 4 , as well as of condensation of 10 and 11 .
A Diverse Array of Cancer-Associated MTOR Mutations Are Hyperactivating and Can Predict Rapamycin Sensitivity (2014)

Genes encoding components of the PI3K-AKT-mTOR signaling axis are frequently mutated in cancer, but few mutations have been characterized in MTOR, the gene encoding the mTOR kinase. Using publicly available tumor genome sequencing data, we generated a comprehensive catalog of mTOR pathway mutations in cancer, identifying 33 MTOR mutations that confer pathway hyperactivation. The mutations cluster in six distinct regions in the C-terminal half of mTOR and occur in multiple cancer types, with one cluster particularly prominent in kidney cancer. The activating mutations do not affect mTOR complex assembly, but a subset reduces binding to the mTOR inhibitor DEPTOR. mTOR complex 1 (mTORC1) signaling in cells expressing various activating mutations remains sensitive to pharmacologic mTOR inhibition, but is partially resistant to nutrient deprivation. Finally, cancer cell lines with hyperactivating MTOR mutations display heightened sensitivity to rapamycin both in culture and in vivo xenografts, suggesting that such mutations confer mTOR pathway dependency.
Synthesis of Novel Diphenyl Ether Herbicides (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of Novel Diphenyl Ether HerbicidesMotoo Sumida, Shinjiro Niwata, Harukazu Fukami, Takaharu Tanaka, Ko Wakabayashi, and Peter BoegerCite this: J. Agric. Food Chem. 1995, 43, 7, 1929–1934Publication Date (Print):July 1, 1995Publication History Published online1 May 2002Published inissue 1 July 1995https://doi.org/10.1021/jf00055a032Request reuse permissionsArticle Views358Altmetric-Citations12LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (764 KB) Get e-Alertsclose Get e-Alerts
Functional characterization of the SIZ/PIAS‐type SUMO E3 ligases, OsSIZ1 and OsSIZ2 in rice (2010)

Sumoylation is a post-translational regulatory process in diverse cellular processes in eukaryotes, involving conjugation/deconjugation of small ubiquitin-like modifier (SUMO) proteins to other proteins thus modifying their function. The PIAS [protein inhibitor of activated signal transducers and activators of transcription (STAT)] and SAP (scaffold attachment factor A/B/acinus/PIAS)/MIZ (SIZ) proteins exhibit SUMO E3-ligase activity that facilitates the conjugation of SUMO proteins to target substrates. Here, we report the isolation and molecular characterization of Oryza sativa SIZ1 (OsSIZ1) and SIZ2 (OsSIZ2), rice homologs of Arabidopsis SIZ1. The rice SIZ proteins are localized to the nucleus and showed sumoylation activities in a tobacco system. Our analysis showed increased amounts of SUMO conjugates associated with environmental stresses such as high and low temperature, NaCl and abscisic acid (ABA) in rice plants. The expression of OsSIZ1 and OsSIZ2 in siz1-2 Arabidopsis plants partially complemented the morphological mutant phenotype and enhanced levels of SUMO conjugates under heat shock conditions. In addition, ABA-hypersensitivity of siz1-2 seed germination was partially suppressed by OsSIZ1 and OsSIZ2. The results suggest that rice SIZ1 and SIZ2 are able to functionally complement Arabidopsis SIZ1 in the SUMO conjugation pathway. Their effects on the Arabidopsis mutant suggest a function for these genes related to stress responses and stress adaptation.
Initial evaluation and comparison of plasma damage to atomic layer carbon materials using conventional and low Te plasma sources (2015)

The ability to achieve atomic layer precision is the utmost goal in the implementation of atomic layer etch technology. Carbon-based materials such as carbon nanotubes (CNTs) and graphene are single atomic layers of carbon with unique properties and, as such, represent the ultimate candidates to study the ability to process with atomic layer precision and assess impact of plasma damage to atomic layer materials. In this work, the authors use these materials to evaluate the atomic layer processing capabilities of electron beam generated plasmas. First, the authors evaluate damage to semiconducting CNTs when exposed to beam-generated plasmas and compare these results against the results using typical plasma used in semiconductor processing. The authors find that the beam generated plasma resulted in significantly lower current degradation in comparison to typical plasmas. Next, the authors evaluated the use of electron beam generated plasmas to process graphene-based devices by functionalizing graphene with fluorine, nitrogen, or oxygen to facilitate atomic layer deposition (ALD). The authors found that all adsorbed species resulted in successful ALD with varying impact on the transconductance of the graphene. Furthermore, the authors compare the ability of both beam generated plasma as well as a conventional low ion energy inductively coupled plasma (ICP) to remove silicon nitride (SiN) deposited on top of the graphene films. Our results indicate that, while both systems can remove SiN, an increase in the D/G ratio from 0.08 for unprocessed graphene to 0.22 to 0.26 for the beam generated plasma, while the ICP yielded values from 0.52 to 1.78. Generally, while some plasma-induced damage was seen for both plasma sources, a much wider process window as well as far less damage to CNTs and graphene was observed when using electron beam generated plasmas.
Enhancement of time-delay measurement accuracy of an insufficient stimulated Brillouin scattering based pulse compression system via lock-in detection (2020)

We demonstrate a novel scheme to increase the accuracy of time-delay measurement of an insufficient stimulated Brillouin scattering (ISBS) based pulse compression system. It is realized by relating the time delay with the pulse compression gain, which is accumulated by a lock-in amplifier (LIA). We theoretically demonstrate that the time delay is proportional to the accumulated gain via the LIA. In the experiment, the pulse compression gain is accumulated through lock-in detection. It narrows down the detection bandwidth and reduces the influence from the broadband noise. The time-delay measurement is completed in a real-time manner and thus provides a possible solution to realizing a high-speed process in the future. The accuracy of measurement of a linear frequency modulated (LFM) signal with a bandwidth of 1 GHz is 1 ns.
Efficient generation of T2*-weighted contrast by interslice echo-shifting for human functional and anatomical imaging at 9.4 Tesla (2014)

The proposed sequence allows for efficient generation of T2*-weighted contrast by combining echo-shifting with an interleaved slice excitation order.
Supplementary Table S1 from miR-409-3p/-5p Promotes Tumorigenesis, Epithelial-to-Mesenchymal Transition, and Bone Metastasis of Human Prostate Cancer (2023)

&lt;p&gt;Gleason scores of the cancer from which the TMA cores were obtained and the miR-409 intensity counts by ISH-QD. None of the TMA cores had any treatment.&lt;/p&gt;
The influence of cell penetrating peptide branching on cellular uptake of QDs (2016)

Semiconductor quantum dots (QDs) serve as a valuable platform for understating the intricacies of nanoparticle cellular uptake and fate for the development of theranostics. Developing novel internalization peptides that maximize cellular uptake while minimizing the amount of peptide is important to allow space on the nanoparticle for other cargo (e.g. drugs). We have designed a range of branched, dendritic internalization peptides composed of polyarginine (Arg<sub>9</sub>) branches (1 to 16 repeats) attached a dendritic wedge based on the sequence WP9G2H6. By attaching these branched dendritic peptides to QD’s, we can study the influence of branching on cellular uptake as a function of time, ratio, and degree of branching.
First steps to define murine amniotic fluid stem cell microenvironment (2016)

Stem cell niche refers to the microenvironment where stem cells reside in living organisms. Several elements define the niche and regulate stem cell characteristics, such as stromal support cells, gap junctions, soluble factors, extracellular matrix proteins, blood vessels and neural inputs. In the last years, different studies demonstrated the presence of cKit<sup>+</sup> cells in human and murine amniotic fluid, which have been defined as amniotic fluid stem (AFS) cells. Firstly, we characterized the murine cKit<sup>+</sup> cells present both in the amniotic fluid and in the amnion. Secondly, to analyze the AFS cell microenvironment, we injected murine YFP<sup>+</sup> embryonic stem cells (ESC) into the amniotic fluid of E13.5 wild type embryos. Four days after transplantation we found that YFP<sup>+</sup> sorted cells maintained the expression of pluripotency markers and that ESC adherent to the amnion were more similar to original ESC in respect to those isolated from the amniotic fluid. Moreover, cytokines evaluation and oxygen concentration analysis revealed in this microenvironment the presence of factors that are considered key regulators in stem cell niches. This is the first indication that AFS cells reside in a microenvironment that possess specific characteristics able to maintain stemness of resident and exogenous stem cells.
Nondestructive Neutron And Gamma-Ray Technologies Applied To GNEP And Safeguards (2007)

In recent years, LLNL has developed methods for diagnosing significant quantities of special nuclear material (SNM). Homeland security problems have recently focused our attention on detection of shielded highly enriched uranium (HEU), which is a weak signal problem. Current and advanced safeguards applications will require working in the opposite extreme of strong but buried signals. We will review some of the technologies that have been developed at LLNL for homeland security applications and discuss how they might be used in support of international safeguards.
Unlicensed Spectrum Access in3GPP (2020)

For several years, the 3GPP approach has built on the use of complementary wireless local area network (WLAN) technology by integrating, interworking or aggregating it under the control of the mobile operator network. This chapter introduces the unlicensed spectrum access technologies from the 3GPP standard point of view. It provides a description of the LTE-WLAN aggregation (LWA), focusing on how WLAN can be aggregated to operate under the control of LTE and how the aggregation of traffic flows works for the radio bearers in the LTE convergence protocol. Special attention is given to describing the necessary network interfaces, how to manage the aggregation in the network, and how the existing networks can be updated to cope with the aggregation. The chapter also describes what in LTE technology needed to change to enable its operation in an unlicensed band. It also summarizes the characteristics of unlicensed technologies and addresses their coexistence.
Synthesis of Substituted Oxindoles from α-Chloroacetanilides via Palladium-Catalyzed C−H Functionalization (2003)

A novel method for the synthesis of oxindoles is described. In the presence of catalytic palladium acetate and 2-(di-tert-butylphosphino)biphenyl, alpha-chloroacetanilides are converted to oxindoles in good to excellent yields with high functional group compatibility using triethylamine as a stoichiometric base. The cyclization is highly regioselective, obviating the need for prefunctionalized arenes. Plausible mechanistic pathways for the reaction are discussed.
In Vivo Ligands of MDA5 and RIG-I in Measles Virus-Infected Cells (2014)

RIG-I-like receptors (RLRs: RIG-I, MDA5 and LGP2) play a major role in the innate immune response against viral infections and detect patterns on viral RNA molecules that are typically absent from host RNA. Upon RNA binding, RLRs trigger a complex downstream signaling cascade resulting in the expression of type I interferons and proinflammatory cytokines. In the past decade extensive efforts were made to elucidate the nature of putative RLR ligands. In vitro and transfection studies identified 5'-triphosphate containing blunt-ended double-strand RNAs as potent RIG-I inducers and these findings were confirmed by next-generation sequencing of RIG-I associated RNAs from virus-infected cells. The nature of RNA ligands of MDA5 is less clear. Several studies suggest that double-stranded RNAs are the preferred agonists for the protein. However, the exact nature of physiological MDA5 ligands from virus-infected cells needs to be elucidated. In this work, we combine a crosslinking technique with next-generation sequencing in order to shed light on MDA5-associated RNAs from human cells infected with measles virus. Our findings suggest that RIG-I and MDA5 associate with AU-rich RNA species originating from the mRNA of the measles virus L gene. Corresponding sequences are poorer activators of ATP-hydrolysis by MDA5 in vitro, suggesting that they result in more stable MDA5 filaments. These data provide a possible model of how AU-rich sequences could activate type I interferon signaling.
The MITLL-AFRL IWSLT 2016 Systems (2016)

Abstract : This report summarizes the MITLL-AFRL MT and ASR systems and the experiments run during the 2016 IWSLT evaluation campaign. Building on lessons learned from previous years results, we refine our ASR systems and examine the explosion of neural machine translation systems and techniques developed in the past year. We experiment with a variety of phrase-based, hierarchical and neural-network approaches in machine translation and utilize system combination to create a composite system with the best characteristics of all MT approaches.
Numerical Analysis of Buoyancy-Induced Flow and Heat Transfer in an Enclosure With Vents (2000)

Abstract This paper reports a numerical study of buoyancy-induced flow and heat transfer in an enclosure with vents. The geometry closely resembles a “set-top-box” application frequently encountered in electronics cooling applications. The heat generating module is modeled as a planar heat source placed on a conducting printed circuit board (PCB). Full 3D and simplified 2D conjugate heat transfer models accounting for conduction and radiation in the solids and conduction and convection in the fluid were used Experiments performed to validate the 3D model have shown excellent comparisons with numerical results. A parametric study involving vent size, power dissipation, number of high conductivity power planes in the PCB has been performed with both the 3D and the 2D models. Although the quantitative results obtained from both types of analyses are similar only under certain conditions, qualitatively, the 2D analysis can be used to obtain useful insights into the complex overall transport mechanisms.
A simple strategy to overcome concentration dependence of photoswitching properties in donor–acceptor Stenhouse adducts (2021)

Photoswitchable donor-acceptor Stenhouse adducts (DASAs) have been reported to exhibit an undesirable concentration dependence, where photoswitching is greatly inhibited with increasing photochrome concentration. Here we show that the use of piperazine-based donor moieties eliminates this concentration dependence and results in complete, rapid and reversible photoswitching behaviour for first generation DASAs, even in chlorinated solvents. Structural data and computational studies reveal proton transfer during isomerisation to the terminal amine rather than the donor amine. The improvement in photoswitching efficiency is attributed to resultant differences in supramolecular association.
Effects of the Selective Estrogen Receptor Modulator Ospemifene on Bone in Rats (2013)

Ospemifene is a non-estrogen agent that exerts tissue-specific estrogen agonistic and weak antagonistic effects (i. e., is a selective estrogen receptor modulator [SERM]). The effects of various once-daily oral doses of ospemifene on bone are examined across 3 studies for 4 or 52 weeks after surgery in the ovariectomized (OVX) rat model of postmenopausal bone loss. Ospemifene treatment reduced the loss of bone mineral content and density observed in untreated OVX rats, significantly increased distal femur bone mineral content at 51 weeks at 25 mg/kg dose compared with untreated OVX rats (p<0.01), and significantly increased trabecular bone mineral density of the distal femur and proximal tibia with 1, 5, or 25 mg/kg doses after 52 weeks. Ospemifene 5 and 25 mg/kg preserved distal femur trabecular structure; trabecular number was significantly increased, whereas trabecular separation and eroded surface values were significantly decreased (all p<0.01). Structural changes associated with ospemifene were accompanied by increased mechanical strength of femurs and 4th lumbar vertebra compared with untreated OVX rats. Ospemifene 10 mg/kg prevented OVX-induced bone loss; trabecular bone volume of distal femurs was increased after 4 weeks. Further, histomorphometric measures revealed decreased bone resorption after 4 weeks of ospemifene treatment, with effects similar to other SERMs (raloxifene and droloxifene). Ospemifene 3 and 10 mg/kg significantly inhibited OVX-induced increases in osteoclast number, and doses ≥0.3 mg/kg dose-dependently reversed the OVX-induced increase in the double-labeled volume:bone volume ratio. These results demonstrate antiresorptive, selective agonist effects of ospemifene on bone that appear similar to raloxifene in this in vivo animal model of estrogen deficiency.
Enzymatic Reactive Absorption and Distillation (2018)

In this work, innovative concepts for reactive separation processes, such as reactive absorption and distillation, that make use of bio-based catalysts, in specific enzymes, are discussed. Enzymatic Reactive Absorption (ERA) and Distillation (ERD) offer potential for energy and investment savings or improved selectivity by exploiting enzyme merits like high enantioselectivity and high reaction rates at milder reaction conditions than chemical catalysts. Potential process equipment, application strategies to supply enzyme for ERA and ERD processes as well as suitable modeling and design approaches are presented. Despite the huge potential, addressing these issues is crucial in order to promote ERA and ERD as vital technologies for process intensification in bio-based industries. The application of the enzyme carbonic anhydrase in an ERA column with common MDEA-based solvent can drastically improve the absorption of CO2 by more than 9-fold. Furthermore, the production of butyl butyrate and enantiomerically pure (R)-1-phenylethyl acetate or (S)-2-pentanol were successfully demonstrated in an ERD column. These processes provided high conversion rates of the substrates and purities of the product stream at milder process conditions compared to conventional processes.
Efficient Enumeration of Instantiations in Bayesian Networks (2013)

Over the past several years Bayesian networks have been applied to a wide variety of problems. A central problem in applying Bayesian networks is that of finding one or more of the most probable instantiations of a network. In this paper we develop an efficient algorithm that incrementally enumerates the instantiations of a Bayesian network in decreasing order of probability. Such enumeration algorithms are applicable in a variety of applications ranging from medical expert systems to model-based diagnosis. Fundamentally, our algorithm is simply performing a lazy enumeration of the sorted list of all instantiations of the network. This insight leads to a very concise algorithm statement which is both easily understood and implemented. We show that for singly connected networks, our algorithm generates the next instantiation in time polynomial in the size of the network. The algorithm extends to arbitrary Bayesian networks using standard conditioning techniques. We empirically evaluate the enumeration algorithm and demonstrate its practicality.
On-Road Vehicle Emissions: Regulations, Costs, and Benefits (1995)

In 1990 Congress passed the Clean Air Act Ammendments, many parts of which deal with motor vehicles. Motor vehicles are the primary source of urban carbon monoxide and are an important source of hydrocarbons and oxides of nitrogen responsible for the formation of photochemical smog and ground-level ozone. Cost estimates for implementing the Acts`s mobil source provisions range up to $12 billion annually. Because of the huge economic cost affecting a ubiquitous transportation method, the authors analyse the scientific basis for the legistation programs. Their work is described here. They conclude that regulatory policies based on a computer model that targets all vehicles equally, with out recognizing the overriding importance of individual maintenance, may not be cost-effective or may be ineffective. 23 refs., 1 fig., 1 tab.