gebhart/patrepeval huggingface dataset:
Specific interactions of chemoattractant factor receptors with G-proteins (1993)

Stimulation of leukocytes with chemoattractant ligands activates phospholipid turnover and calcium release, ultimately leading to chemotaxis, degranulation, and the inflammatory response. The leukocyte response to these ligands is transduced by the interaction of transmembrane receptors with GTP-binding regulatory proteins (G-proteins). To examine the mechanisms of signal transduction by these receptors, we transfected cDNA clones encoding the receptors for the active cleavage product of the fifth component of complement (C5a) and platelet-activating factor (PAF) into COS-7 cells, then measured the production of inositol phosphates (IP) in response to stimulation with these chemoattractant ligands. Cells transfected with the C5a receptor showed no increase in IP production when stimulated with ligand (5-120 nM). However, in cells co-transfected with these receptors and with the cDNA for G alpha 16, a G-protein alpha subunit that is specific to cells of hematopoietic lineage, addition of ligand caused up to a 5-fold increase in IP production. This interaction was specific, as co-transfection of receptors with the G-proteins G alpha q or G alpha 11 did not allow ligand-dependent increase in IP production. In contrast, ligand-dependent activation of IP production was seen in COS cells transfected solely with the PAF receptor. These results indicate that the C5a receptor utilizes signaling pathways distinct from the PAF receptor and suggest that a pertussis toxin-resistant G-protein, G alpha 16, may play a role in the leukocyte response to inflammatory ligands.
Gene Polymorphism of Angiotensin II Type 1 and Type 2 Receptors (2013)

Renin-angiotensin system (RAS) plays a key role in pathogenesis of cardiovascular disease and in the responsiveness for various types of medications. A large number of genetic investigations have been carried out to examine the association between gene variants of RAS and predisposition to cardiovascular diseases, such as hypertension, coronary artery disease and stroke. Even though the major results were obtained from genetic association studies of angiotensinogen or angiotensin converting enzyme, unique findings were also elucidated from investigations concerning single nucleotide polymorphisms (SNPs) of 2 types of angioteinsin II receptor genes denoted as AGTR1 and AGTR2. Both genes have many SNPs in the coding and its flanking regions but most of the studies used A1166C polymorphism of AGTR1 and G1675A polymorphism of AGTR2. In the subjects with C1166 allele of AGTR1, several investigations reported increased risk for coronary artery disease, ischemic stroke, heart failure and end-stage renal disease but not for hypertension. Interestingly, a few papers pointed out the possibility that A1166C modulates the efficacy of RAS inhibitors. In the genetic analysis of AGTR2, G1675 allele carriers had increased risk for left ventricular hypertrophy, renal insufficiency and modulated hemodynamic response to RAS inhibitors. In this review, we summarized previous investigations concerning the genetic aspects of AGTR1 and AGTR2 to consider the clinical utility of SNPs of these receptors.
The Willingness to Pay—Willingness to Accept Gap, the “Endowment Effect,” Subject Misconceptions, and Experimental Procedures for Eliciting Valuations: Reply (2011)

Isoni, Loomes, and Sugden (2011) assert that Plott and Zeiler (2005) reported inaccurate results. Placing ILS's selective quotes into context demonstrates otherwise. Additionally, examining the data closely yields three conclusions. First, all mug data reject endowment effect theory. Second, lottery gaps are associated with unstable attitudes toward uncertainty, a finding consistent with PZ's (2005) lottery data description, explicit warnings about procedure limitations and the data supplement, which reports the lottery data and cautions. Third, lottery outcome beliefs are influenced by whether WTP or WTA is reported, suggesting that changing beliefs, as opposed to the shape of preferences, produce lottery gaps. (JEL C91)
ChemInform Abstract: Aminoalkylation of Cyclic and Acyclic Alkyl Vinyl Ethers. (1999)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Probing the Reaction Zone of Nanolaminates at ∼μs Time and ∼μm Spatial Resolution (2020)

Reactive nanolaminates are a high-energy-density configuration for energetics that have been widely studied for their tunable energy release rates. In this study, we characterized Al/CuO nanolaminate reactions with different fuel/oxidizer ratios and bilayer thicknesses using both macro- and microscale high-speed imaging/pyrometry. Under microscopic imaging, we observe significant corrugation (the ratio of the total geometrical length of the flame to the width of the sample in the direction perpendicular to propagation) of the flame, which can increase the reaction surface area by as much as a factor of 3. This in turn manifests itself as an increase in the global burn rate (total nanolaminate film length/total burn time). We find that the global burn rate can be predicted as the product of the microburn rate (local vector burn rate at the microscopic scale) and the corrugation. These corrugation effects primarily impact fuel-rich conditions, resulting in higher global burn rates. We find that the reaction zone has a thickness of ∼150 μm. Finally, we present a 3D rendering of what we believe the reaction zone looks like, based on the results from in-operando observation and SEM cross-sectional imaging.
Full-Length Recombinant Human SCF1-165 Is More Thermostable than the Truncated SCF1-141 Form (2014)

Human stem cell factor initiates a diverse array of cellular responses, including hematopoiesis, cell proliferation, differentiation, migration and survival. To explore the relationship between its structure and function, we produced recombinant soluble human stem cell factor1-165 (wild type) and human stem cell factor1-141 (C-terminal truncated) in a yeast expression system and compared their biological activities and thermal stabilities. The biological activity of the two proteins was measured as a function of TF-1 cell viability and effects on downstream signaling targets after incubation. We found that these proteins enhanced cell viability and downstream signaling to a similar extent, in a dose-dependent manner. The biological activity of recombinant human stem cell factor1-165 was significantly greater than that of recombinant human stem cell factor1-141 after heating the proteins (100 ng/mL) at 25-110°C for 10 minutes (P<0.05 for all temperatures). In addition, circular dichroism spectral analysis indicated that β-sheet structures were altered in recombinant human stem cell factor1-141 but not recombinant human stem cell factor1-165 after heating at 90°C for 15 or 30 min. Molecular modeling and limited proteolytic digestion were also used to compare the thermo stability between human stem cell factor1-165 and human stem cell factor1-141. Together, these data indicate that stem cell factor1-165 is more thermostable than stem cell factor1-141.
Functional Structures of AgOxThin Film for Near-Field Recording (2003)

The optical processes of the AgOx thin film under various laser pumping pulses are studied using an optical pump-probe system. Detailed changes of the reflectance measurements clearly show the different transition process of the dissociation of the AgOx thin film. The structures of dots and rings of written spots are observed in these processes. Two possible models of the distribution of the dissociated silver are proposed.
Modeling and simulation of resistive switching devices (2013)

As conventional memory concepts are approaching their physical scaling limits, novel memory device concepts for highly scalable, ultrafast, energy efficient, nonvolatile memory are demanded. Resistive switching devices, which rely on nanoionic redox phenomena, are potential candidates for the use in resistive random access memories (ReRAMs) or logic applications. By applying appropriate electrical stimuli they can be switched back and forth between different resistance states, which encode the digital information. Based on their physical nature three different resistive switching mechanisms can be distinguished: the electrochemical mechanism (ECM), the valence-change mechanism (VCM) and the thermochemical mechanism (TCM). While the basic principles of the switching processes are well understood, many details are still unknown or under discussion. Especially, the origin of the switching kinetics in VCM cells and the physical origin of the multilevel programming capability in ECM cells need to be clarified. To address these open questions simulation models can be employed. However, until now there are no simulation models available that can answer these questions. In this thesis, physics-based simulation models of ECM and VCM devices are developed to address these questions. For this, the corresponding simulation results are compared to experimental data. A physics-based dynamic 1D compact model for resistive switching in ECM cells is presented. Simulations based on this model were performed to investigate fundamental phenomena as the multilevel switching capabilities and the nonlinear switching kinetics. The former is realized by modulation of a tunneling gap between one electrode and a growing filament within the insulating layer. By comparison to experimental data, this model was validated. Further simulations were performed to investigate the material impact on the resistive switching properties. This model was further simplified to an analytical one. Analytical expressions were derived, which allow predicting switching times and currents. Finally, the 1D model is extended to a 2D finite elements simulation model. With this approach polyfilamentary growth and the unipolar switching phenomenon in ECM cells at very low resistances were investigated and discussed. Regarding the VCM cell, an electro-thermal finite elements simulation model was developed to explain the origin of the nonlinear switching kinetics. While it is widely accepted that the resistive switching is triggered by the migration of oxygen vacancies in the insulating layer, it is yet unclear whether the motion is temperature- or field-enhanced. A comparison of the model and the experimental data revealed that the nonlinear switching kinetics is predominantly caused by temperature-accelerated drift of oxygen vacancies. Further simulations were performed to investigate the scaling potential of VCM cells. It could be demonstrated that the VCM memory concept allows cell sizes in the low nanometer regime. In addition, the derived model provides essential rules for an optimized cell design.
NF-κB drives epithelial-mesenchymal mechanisms of lung fibrosis in a translational lung cell model (2022)

In the progression phase of idiopathic pulmonary fibrosis (IPF), the normal alveolar structure of the lung is lost and replaced by remodeled fibrotic tissue and by bronchiolized cystic airspaces. Although these are characteristic features of IPF, knowledge of specific interactions between these pathological processes is limited. Here, the interaction of lung epithelial and lung mesenchymal cells was investigated in a coculture model of human primary airway epithelial cells (EC) and lung fibroblasts (FB). Single-cell RNA sequencing revealed that the starting EC population was heterogenous and enriched for cells with a basal cell signature. Furthermore, fractions of the initial EC and FB populations adopted distinct pro-fibrotic cell differentiation states upon cocultivation, resembling specific cell populations that were previously identified in lungs of patients with IPF. Transcriptomic analysis revealed active NF-κB signaling early in the cocultured EC and FB, and the identified NF-κB expression signatures were found in “HAS1 High FB” and “PLIN2+ FB” populations from IPF patient lungs. Pharmacological blockade of NF-κB signaling attenuated specific phenotypic changes of EC and prevented FB-mediated interleukin-6, interleukin-8, and CXC chemokine ligand 6 cytokine secretion, as well as collagen α-1(I) chain and α–smooth muscle actin accumulation. Thus, we identified NF-κB as a potential mediator, linking epithelial pathobiology with fibrogenesis.
A simplified model for nonlinear cross-phase modulation in hybrid optical coherent system (2009)

Cross-phase modulation (XPM) has been considered as one of the ultimate obstacles for optical coherent dense wavelength division multiplexing (DWDM) systems. In order to facilitate the XPM analysis, a simplified model was proposed. The model reduced the distributed XPM phenomena to a lumped phase modulation. The XPM phase noise was generated by a linear system which was determined by the DWDM system parameters and whose inputs were undistorted pump channel intensity waveforms. The model limitations induced by the lumped phase modulation and undistorted pumps approximations were intensively discussed and verified. The simplified model showed a good agreement with simulations and experiments for a typical hybrid optical coherent system. Various XPM phenomena were explained by the proposed model.
Journal of Drug Targeting Life Time Achievement Award for Prof. Robert Langer (2015)

"Journal of Drug Targeting Life Time Achievement Award for Prof. Robert Langer." Journal of Drug Targeting, 23(7-8), p. 579
Centrifugal Electrospinning Enables the Production of Meshes of Ultrathin Polymer Fibers (2020)

Nanomaterials show extraordinary properties and, among them, polymeric nanofibers are of high interest for several applications. Here, the applicability of a high throughput production system is investigated by combining solution-based electrospinning with centrifugal spinning. Ultrathin nanofibers were generated with diameters in the tens of nanometer regime using polyethylene glycol and polylactic acid, and the productivity of the highly interconnected nanofiber nonwoven meshes was orders of magnitude faster compared to that of materials made with traditional electrospinning methods. Such a manufactured layer of nanofibers shows great potential for filter applications, for example.
Use of a rapid and highly sensitive fluorescamine-based procedure for the assay of plasma lipoproteins. (1988)

A rapid and sensitive method for determining protein concentrations using fluorescamine has been characterized for use in the analysis of intact lipoproteins. It was shown that there is no interference with the assay due to the presence of lipid-associated turbidity or primary amine content. The assay was shown to be sensitive to as little as 0.3 microgram of lipoprotein and to yield similar results when compared to the Lowry method.
H2O2 aktiviert posttranskriptionell die Transferrin-vermittelte Eisenaufnahme: ein Pathomechanismus der entzündungs-vermittelten Eisenakkumulation? (2015)

Eisen ist nicht nur ein essentieller Wachstumsfaktor, sondern auch ein starkes Zellgift. Die Toxizität wird dabei vor allem über die in Verbindung mit H2O2 gebildeten Hydroxylradikale vermittelt (Fenton-Chemie). Bei akuten und chronischen Entzündungen kommt es neben einem vermehrten oxidativen Stress typischerweise zum Abfall des Serumeisens und zur Erhöhung der Eisenspiegel im Gewebe (z.B. chronische Hepatitis C). Die Mechanismen dieser (patho)physiologisch bedeutsamen Eisenregulation sind bis heute ungeklärt. Wir haben früher zeigen können, das der zentrale Eisenschalter IRP-1 vor allem durch extrazelluläres H2O2 rasch sowohl in Zellkulturen als auch in der intakten Leber aktiviert wird.
miR-194 Is a Marker of Hepatic Epithelial Cells and Suppresses Metastasis of Liver Cancer Cells in Mice (2010)

These results support a role of miR-194, which is specifically expressed in liver parenchymal cells, in preventing liver cancer cell metastasis.
Cross-Domain Image Retrieval with a Dual Attribute-Aware Ranking Network (2015)

We address the problem of cross-domain image retrieval, considering the following practical application: given a user photo depicting a clothing image, our goal is to retrieve the same or attribute-similar clothing items from online shopping stores. This is a challenging problem due to the large discrepancy between online shopping images, usually taken in ideal lighting/pose/background conditions, and user photos captured in uncontrolled conditions. To address this problem, we propose a Dual Attribute-aware Ranking Network (DARN) for retrieval feature learning. More specifically, DARN consists of two sub-networks, one for each domain, whose retrieval feature representations are driven by semantic attribute learning. We show that this attribute-guided learning is a key factor for retrieval accuracy improvement. In addition, to further align with the nature of the retrieval problem, we impose a triplet visual similarity constraint for learning to rank across the two subnetworks. Another contribution of our work is a large-scale dataset which makes the network learning feasible. We exploit customer review websites to crawl a large set of online shopping images and corresponding offline user photos with fine-grained clothing attributes, i.e., around 450,000 online shopping images and about 90,000 exact offline counterpart images of those online ones. All these images are collected from real-world consumer websites reflecting the diversity of the data modality, which makes this dataset unique and rare in the academic community. We extensively evaluate the retrieval performance of networks in different configurations. The top-20 retrieval accuracy is doubled when using the proposed DARN other than the current popular solution using pre-trained CNN features only (0.570 vs. 0.268).
Bt Resistance inHelicoverpa zea1(Boddie): from Biology to Monitoring (2010)

Results from conventional resistance monitoring and F2 screens have found no evidence of major resistance alleles to Cry1Ac in the bollworm, Helicoverpa zea (Boddie), in US cotton, Gossypium hirsutum L., fields. Furthermore, sentinel field plot results show no changes in field performance of Cry1Ac cotton despite 14 years of commercial use. Although insecticide-resistant management practices such as refugia, insecticide oversprays, and pyramided traits in Bt cotton have likely contributed to the lack of observed resistance development and changes in field efficacy, fitness costs associated with the development of Cry1Ac resistance in bollworm probably also have contributed to this outcome. This paper discusses various fitness costs associated with Cry1Ac resistance in bollworm and concludes with a discussion of how exclusive use of diet-based laboratory bioassays may underestimate their role in delaying development of resistance to Bt.
Allelic Variants of Human Melatonin 1A Receptor in Patients with Familial Adolescent Idiopathic Scoliosis (2003)

The results of this study demonstrated no evidence of linkage to chromosome 4q and no mutations in the coding region of the gene for human melatonin receptor. The identification of variants in the human melatonin receptor could provide a useful tool for testing the gene in the predisposition to various other melatonin-related disorders and for clarifying the role of melatonin in adolescent idiopathic scoliosis.
Digital Predistortion Algorithm of Advance Coherent Modulation Schemes Enabling Radio over Fiber for Access Networks (2021)

Digital predistortion algorithms are demonstrated for 5G back-bone optical systems employing 64-QAM and 256-QAM advance modulation formats. The performance of the digitally linearized systems is compared experimentally and using simulation with standard coherent transmission systems.
DNA intercalator BMH-21 inhibits RNA polymerase I independent of DNA damage response (2014)

DNA intercalation is a major therapeutic modality for cancer therapeutic drugs. The therapeutic activity comes at a cost of normal tissue toxicity and genotoxicity. We have recently described a planar heterocyclic small molecule DNA intercalator, BMH-21, that binds ribosomal DNA and inhibits RNA polymerase I (Pol I) transcription. Despite DNA intercalation, BMH-21 does not cause phosphorylation of H2AX, a key biomarker activated in DNA damage stress. Here we assessed whether BMH-21 activity towards expression and localization of Pol I marker proteins depends on DNA damage signaling and repair pathways. We show that BMH-21 effects on the nucleolar stress response were independent of major DNA damage associated PI3-kinase pathways, ATM, ATR and DNA-PKcs. However, testing a series of BMH-21 derivatives with alterations in its N,N-dimethylaminocarboxamide arm showed that several derivatives had acquired the property to activate ATM- and DNA-PKcs -dependent damage sensing and repair pathways while their ability to cause nucleolar stress and affect cell viability was greatly reduced. The data show that BMH-21 is a chemically unique DNA intercalator that has high bioactivity towards Pol I inhibition without activation or dependence of DNA damage stress. The findings also show that interference with DNA and DNA metabolic processes can be exploited therapeutically without causing DNA damage.
Real-time super-resolution Sound Source Localization for robots (2012)

Sound Source Localization (SSL) is an essential function for robot audition and yields the location and number of sound sources, which are utilized for post-processes such as sound source separation. SSL for a robot in a real environment mainly requires noise-robustness, high resolution and real-time processing. A technique using microphone array processing, that is, Multiple Signal Classification based on Standard EigenValue Decomposition (SEVD-MUSIC) is commonly used for localization. We improved its robustness against noise with high power by incorporating Generalized EigenValue Decomposition (GEVD). However, GEVD-based MUSIC (GEVD-MUSIC) has mainly two issues: 1) the resolution of pre-measured Transfer Functions (TFs) determines the resolution of SSL, 2) its computational cost is expensive for real-time processing. For the first issue, we propose a TF interpolation method integrating time-domain-based and frequency-domain-based interpolation. The interpolation achieves super-resolution SSL, whose resolution is higher than that of the pre-measured TFs. For the second issue, we propose two methods, MUSIC based on Generalized Singular Value Decomposition (GSVD-MUSIC), and Hierarchical SSL (H-SSL). GSVD-MUSIC drastically reduces the computational cost while maintaining noise-robustness in localization. H-SSL also reduces the computational cost by introducing a hierarchical search algorithm instead of using greedy search in localization. These techniques are integrated into an SSL system using a robot embedded microphone array. The experimental result showed: the proposed interpolation achieved approximately 1 degree resolution although we have only TFs at 30 degree intervals, GSVD-MUSIC attained 46.4% and 40.6% of the computational cost compared to SEVD-MUSIC and GEVD-MUSIC, respectively, H-SSL reduces 59.2% computational cost in localization of a single sound source.
Changes in Plasma Copeptin, the C-Terminal Portion of Arginine Vasopressin during Water Deprivation and Excess in Healthy Subjects (2007)

Copeptin shows identical changes during disordered water states as previously shown for AVP. It might be a reliable marker of AVP secretion and substitute for the measurement of circulating AVP levels in clinical routine.