gebhart/patrepeval huggingface dataset:
Macromodeling of Nonlinear Transistor-Level Receiver Circuits (2006)

In this paper, a modeling methodology for macromodeling transistor-level receiver circuits has been presented. A few receiver modeling techniques have been proposed in the past, but these modeling techniques only address the loading effect of the receiver circuits, i.e., the input characteristics of the receivers. In this paper, a modeling methodology that addresses both the loading effect as well as the output characteristics of the receiver has been proposed. This modeling technique is simple, accurate, and has huge computational speed-up over transistor-level receiver circuits. To model the input characteristics of the receiver, spline function with finite time difference (SFWFTD) and recurrent neural network (RNN) modeling methods have been used. The output characteristics of the receiver are modeled using a combination of receiver static characteristics and a delay element that takes into account the timing delay of the receiver. The accuracy of the modeling approach has been tested on some test cases and results show good accuracy and substantial speed-up compare to transistor-level receiver circuits. The proposed modeling technique has been extended to multiple ports to estimate sensitive effects like simultaneous switching noise (SSN) when multiple receivers are switching.
Electrical Probing Test for Characterizing Wideband Optical Transceiving Devices with Self-Reference and On-Chip Capability (2018)

An electrical probing test method is proposed based on serial or parallel modulation mixing scheme for characterizing wideband optical transceiving devices including high-speed semiconductor laser diodes, electro-absorption modulators, photodetectors, and Mach-Zehnder modulators. The method enables self-referenced frequency responses measurement, such as modulation index of semiconductor laser diodes, modulation index of electro-absorption modulators, responsivity of photodetectors, and modulation index and half-wave voltage of dual-parallel Mach-Zehnder modulators, dual-drive Mach-Zehnder modulators, and push-pull Mach-Zehnder modulators. In the demonstration, the experimental results are compared to those obtained with the conventional optical or electrical methods for accuracy. The full-electrical test nature is promising for microwave characterization of wafer level devices and circuits with fully integrated transceiving components on chip.
Cell Delivery: Enhanced In Vivo Delivery of Stem Cells using Microporous Annealed Particle Scaffolds (Small 39/2019) (2019)

In article number 1903147, Philip O. Scumpia, Dino Di Carlo, and co-workers co-inject microgel particles with cells, which are covalently linked in situ to form a microporous scaffold for enhanced cell therapies.
A Tunable 5–7 GHz Distributed Active Quasi-Circulator With 18-dBm Output Power in CMOS SOI (2017)

Circulators and quasi-circulators (QCs) have a tradeoff between maximum isolation and bandwidth. We present a tunable distributed active circuit to achieve high isolation over a wide frequency range. The reported QC provides more than 40-dB suppression between transmit (TX) output signal at the antenna and TX leakage into the receive (RX) port over a tuning range of 5.3-7.3 GHz in small-signal operation. Large-signal TX-RX suppression is optimized across power level. The TX output achieves peak power of 18 dBm and 12% power-added efficiency at 6.3 GHz. The QC is designed in a high-resistivity 45-nm CMOS silicon-on-insulator process and occupies an area of 1.62 mm × 0.97 mm.
Lymphokine-mediated induction of antigen-presenting ability in thymic stromal cells. (1987)

We have established and characterized long term thymic stromal cultures from BALB/c (H-2d) and CBA/J (H-2k) mice. All cultures contained multiple adherent cell types, whereas some also contained thymic macrophages (TM). Culture supernatants from all cultures tested contained macrophage colony-stimulating factor activity, whereas only cultures with TM had soluble or membrane-associated interleukin (IL)-1. However, a thymic epithelial cell line (3D . 1), cloned from one of these cultures, produced IL-1 bioactivity. Further analysis confirmed the production of IL-1 alpha mRNA by the epithelial cell. No IL-2 or IL-4 (formerly called B cell stimulatory factor 1) activity was detected in any of the cultures. Antigen-presenting (AP) ability was determined using the chicken ovalbumin (OVA)-specific, I-Ad-restricted T cell hybridoma 3DO-18.3. Harvested TM exhibited antigen-specific, Ia-restricted AP ability which was enhanced by IL-4 as well as interferon-gamma (IFN-gamma). In contrast, AP ability was detected in non-macrophage stromal cell cultures (NMSC) only after preincubation with IFN-gamma. AP by preinduced NMSC was also Ia-restricted and could be blocked by anti-I-Ad antibodies. Since the T cell receptor of 3DO-18.3 is known to recognize a peptide produced by CNBr degradation of OVA, these observations suggest that both TM and NMSC can process OVA to produce this peptide. Glutaraldehyde-fixation experiments confirmed that NMSC must process native OVA into antigenic peptides for successful AP. Assays using several cloned stromal cell lines of different lineages suggested that only epithelial cells could be induced with IFN-gamma to exhibit competent AP. Given the possible role for IFN-gamma in the maintenance of Ia in the thymus, we investigated whether IFN-gamma production could be ascribed to a subpopulation of thymocytes. Culture supernatants from calcium ionophore and phorbol ester-stimulated peanut agglutinin-negative, but not peanut agglutinin-positive, thymocytes induced AP ability in NMSC. Thus, some thymocytes can produce an Ia-inducing lymphokine (most likely IFN-gamma) which may play an important role in T cell ontogeny through its effects on both thymic macrophages and thymic epithelial cells.
Iodine‐transfer polymerization and CuAAC “click” chemistry: A versatile approach toward poly(vinylidene fluoride)‐based amphiphilic triblock terpolymers (2019)

ABSTRACT This study presents the synthesis and properties of linear PVDF‐based amphiphilic triblock terpolymers with PS and PEO, [PVDF‐ b ‐PS‐ b ‐PEO], by adopting a procedure that involves: (a) iodine‐transfer polymerization (ITP) of VDF with 1‐iodoperfluorohexane (C 6 F 13 I) serving as chain‐transfer agent (CTA) to afford C 6 F 13 ‐PVDF‐I, (b) ITP of styrene with the C 6 F 13 ‐PVDF‐I macromolecular‐CTA to obtain C 6 F 13 ‐PVDF‐ b ‐PS‐I diblock copolymer, (c) end‐group exchange from iodo‐ to azido‐group by nucleophilic substitution reaction with NaN 3, and (d) copper‐catalyzed azide‐alkyne cycloaddition (CuAAC) with alkyne‐terminated PEO to achieve C 6 F 13 ‐PVDF‐ b ‐PS‐ b ‐PEO triblock terpolymers. The 1 H and 19 F NMR spectroscopy confirmed the presence of all blocks, while gel permeation chromatography traces showed the living nature of ITP technique. The self‐assembly of these terpolymers was investigated in films (atomic force microscopy and DSC), as well as in aqueous and organic solvents (DLS). The analysis of crystalline phases based on the FTIR spectroscopy indicated the conversion of PVDF α‐phase into α + β‐phases and β + γ‐phases upon the incorporation of PS and PEO blocks, respectively. The synthesized amphiphilic copolymers were evaluated (fluorescence spectroscopy) as carriers of small hydrophobic molecules in water. © 2019 Wiley Periodicals, Inc. J. Polym. Sci. 2020 , 58 , 163–171
Quality of red blood cells washed using a second wash sequence on an automated cell processor (2015)

Using the ACP 215, a double-wash procedure for the production of components for IgA-deficient recipients from either BC or WBF RBCs was developed and validated.
ORIGINAL RESEARCH: A dedicated vector for efficient library construction and high throughput screening in the hyphal fungus Chrysosporium lucknowense (2007)

A self-replicating vector was designed that enables the construction of complex libraries in the fungus Chrysosporium lucknowense. The circular vector is linearized in vivo and results in a transformation frequency up to 13,000 transformants/μg of plasmid DNA. Upon prolonged cultivation of the transformants in liquid medium, the linearized vector finally integrates, mainly as a single copy, at the telomere associated region of the chromosomes. Screening for colonies with compact filamentous growth on agar plates resulted in a C. lucknowense strain with significantly reduced viscosity during fermentation. Further medium modification resulted in conditions that enable cultivation, replication, and screening of a large collection of fungal transformants in microtiter plates. This paper describes the successful integration of library construction and screening in an automated setup using different configurations for a hyphal fungal system.
Symmetry of Charge Partitioning in Collisional and UV Photon-Induced Dissociation of Protein Assemblies (2016)

Tandem mass spectrometry can provide structural information on intact protein assemblies, generating mass fingerprints indicative of the stoichiometry and quaternary arrangement of the subunits. However, in such experiments, collision-induced dissociation yields restricted information due to simultaneous subunit unfolding, charge rearrangement, and subsequent ejection of a highly charged unfolded single subunit. Alternative fragmentation strategies can potentially overcome this and supply a deeper level of structural detail. Here, we implemented ultraviolet photodissociation (UVPD) on an Orbitrap mass spectrometer optimized for native MS and benchmark its performance to HCD fragmentation using various protein oligomers. We investigated dimeric β-lactoglobulin, dimeric superoxide dismutase, dimeric and tetrameric concanavalin A, and heptameric GroES and Gp31; ranging in molecular weight from 32 to 102 kDa. We find that, for the investigated systems, UVPD produces more symmetric charge partitioning than HCD. While HCD spectra show sporadic fragmentation over the full protein backbone sequence of the subunits with a bias toward fragmenting labile bonds, UVPD spectra provided higher sequence coverage. Taken together, we conclude that UVPD is a strong addition to the toolbox of fragmentation methods for top-down proteomics experiments, especially for native protein assemblies.
Antitumor Effects of a New Retinoate of the Fungal Cytotoxin Illudin M in Brain Tumor Models (2022)

While the fungal metabolite illudin M (<b>1</b>) is indiscriminately cytotoxic in cancer and non-malignant cells, its retinoate <b>2</b> showed a greater selectivity for the former, especially in a cerebral context. Illudin M killed malignant glioma cells as well as primary neurons and astrocytes at similarly low concentrations and destroyed their microtubule and glial fibrillary acidic protein (GFAP) networks. In contrast, the ester <b>2</b> was distinctly more cytotoxic in highly dedifferentiated U87 glioma cells than in neurons, which were even stimulated to enhanced growth. This was also observed in co-cultures of neurons with U87 cells where conjugate <b>2</b> eventually killed them by induction of differentiation based on the activation of nuclear receptors, which bind to retinoid-responsive elements (RARE). Hence, illudin M retinoate <b>2</b> appears to be a promising drug candidate.
Percutaneous nephrostolithotomy vs open surgery for renal calculi. A comparative study (1985)

To evaluate the impact of the percutaneous removal of renal calculi we compared 41 patients who had undergone open renal surgery to 88 patients who had a one- or two-stage percutaneous nephrostolithotomy for symptomatic urolithiasis. The two groups were similar with regard to stone size, stone location, treatment success, and complication rates. However, for patients with calculi 2.5 cm or smaller in diameter, the percutaneous procedure resulted in lower postoperative morbidity, more rapid convalescence, less hospital cost, and greater patient satisfaction. For patients with calculi larger than 2.5 cm in diameter, the percutaneous approach was slightly more expensive than open surgery but resulted in a markedly shortened convalescent period. In our experience, percutaneous nephrostolithotomy performed in a single stage under assisted local anesthesia was the most efficacious and least expensive of the percutaneous approaches. (<i>JAMA</i>1985;254:1054-1058)
COVA1-18 neutralizing antibody protects against SARS-CoV-2 in three preclinical models (2021)

Effective treatments against Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) are urgently needed. Monoclonal antibodies have shown promising results in patients. Here, we evaluate the in vivo prophylactic and therapeutic effect of COVA1-18, a neutralizing antibody highly potent against the B.1.1.7 isolate. In both prophylactic and therapeutic settings, SARS-CoV-2 remains undetectable in the lungs of treated hACE2 mice. Therapeutic treatment also causes a reduction in viral loads in the lungs of Syrian hamsters. When administered at 10 mg kg-1 one day prior to a high dose SARS-CoV-2 challenge in cynomolgus macaques, COVA1-18 shows very strong antiviral activity in the upper respiratory compartments. Using a mathematical model, we estimate that COVA1-18 reduces viral infectivity by more than 95% in these compartments, preventing lymphopenia and extensive lung lesions. Our findings demonstrate that COVA1-18 has a strong antiviral activity in three preclinical models and could be a valuable candidate for further clinical evaluation.
Estimating the Current Mean of a Process Subject to Abrupt Changes (1995)

This article discusses estimation of the current process mean in situations in which this parameter is subject to abrupt changes of unpredictable magnitude at some unknown points in time. It introduces performance criteria for this estimation problem and discusses in detail the relative merits of several estimation procedures. I show that an estimate based on exponentially weighted moving average of past observations has optimality properties within the class of linear estimators, and I propose alternative estimating procedures to overcome its limitations. I consider two primary types of estimation procedures, Markovian estimators, in which the current estimate is obtained as a function of the previous estimate and the most current data point, and adaptive estimators, based on identification of the most recent changepoint. We give several examples that illustrate the use of the proposed techniques.
P059 Klebicin as a novel therapy for Klebsiella infection management (2023)

Abstract Background Common infections are becoming almost untreatable, all because of the emergence and spread of drug-resistant pathogens. The highest risk is carried by rapidly spreading multi and pan-resistant bacteria that cause infections untreatable with existing antibiotics. Nowadays the biggest concern is Klebsiella bacteria which reportedly has resistance percentages of 25% or higher for third-generation antimicrobial medicine (WHO, 2022). Orally delivered recombinant bacteriocins, like klebicins, could be employed as oral antimicrobials to eradicate multidrug-resistant Klebsiella from the intestinal tract before hospitalization. Methods This study aimed to investigate the antimicrobial efficacy of orally delivered Eudragit - coated klebicin (KvarM) in a murine gastrointestinal tract model of K. pneumoniae infection. Biomodels were used (5 animals/group) to test the antimicrobial efficacy of orally delivered klebicin KvarM: vehicle-only control group (K. pneumoniae, Eudragit coating) and experimental group with K. pneumoniae and KvarM coated to be released in small and large intestines. Faecal samples were used for the analysis of Klebsiella haemolysin gene (khe), which is highly specific for Klebsiella species, using RT-PCR. Results The gastrointestinal model of K. pneumoniae infection in mice was achieved per os without any antibiotic pretreatment following the introduction of coated klebicin KvarM therapy once per day for four days. In the first experimental group, the amounts of K. pneumoniae changed from 6,15 x 106 CFU/50 mg after K. pneumoniae administration to 4,68 x 106 CFU/50 mg after the last dose of therapy (23,9 percent efficacy) in the experimental group. Nonetheless, there was a significant difference between the vehicle-control group and the first group after klebicin administration (p=0,023). Conclusion Our study shows that the K. pneumoniae infection in the intestinal tract of mice was reduced by 23,9 percent and can be significantly lowered in bacterial counts using orally delivered klebicin.
Red Blood Cell Surface Charge and Alcian Blue Binding (1987)

Journal Article Red Blood Cell Surface Charge and Alcian Blue Binding Get access J. G. J. v. d. Winkel, J. G. J. v. d. Winkel Department of Medicine, Division of Nephrology, University HospitalNijmegen, The Netherlands Search for other works by this author on: Oxford Academic PubMed Google Scholar J. F. M. Wetzels, J. F. M. Wetzels Department of Medicine, Division of Nephrology, University HospitalNijmegen, The Netherlands Search for other works by this author on: Oxford Academic PubMed Google Scholar J. L. P. van Duijnhoven, J. L. P. van Duijnhoven Department of Medicine, Division of Nephrology, University HospitalNijmegen, The Netherlands Search for other works by this author on: Oxford Academic PubMed Google Scholar R. A. P. Koene, R. A. P. Koene Department of Medicine, Division of Nephrology, University HospitalNijmegen, The Netherlands Search for other works by this author on: Oxford Academic PubMed Google Scholar P. J. A. Capel P. J. A. Capel Department of Medicine, Division of Nephrology, University HospitalNijmegen, The Netherlands Search for other works by this author on: Oxford Academic PubMed Google Scholar Nephrology Dialysis Transplantation, Volume 2, Issue 4, 1987, Pages 280–281, https://doi.org/10.1093/oxfordjournals.ndt.a091559 Published: 01 January 1987
SWT based bearing fault detection using frequency spectral subtraction of stator current with and without an adaptive filter (2017)

This paper presents a comparative analysis in detecting bearing faults of a 3-phase induction motor using frequency spectral subtraction of stator current with and without an adaptive filter (Wiener filter). At incipient stage of fault, supremacy of healthy components is a major issue in current based monitoring. To overcome this problem, it is essential to suppress the effect of healthy components to estimate the embryonic fault component in stator current. Therefore, in this paper Frequency Spectral Subtraction (FSS) is carried out to remove the above said healthy components to highlight bearing fault component in stator current. FSS is carried out by two ways: one way is frequency components of both healthy and fault components are obtained using FFT and are subtracted directly. On the other hand, the healthy components are predicted using an adaptive filter and are removed in a real time fashion. In both the cases, the subtracted signal is further analyzed using Stationary wavelet transform (SWT) to get better resolution and compared. The results obtained using proposed topology is compared with conventional vibration analysis performed on 1.5kW test bed.
A general method to select for M13 clones carrying base pair substitution mutants constructedin vitro (1983)

In this paper we describe a method to select base pair substitution mutants constructed in vitro. The mutagenesis is performed by forcing mistakes during in vitro synthesis from a primer annealed to a single stranded DNA template. The selection is based on the fact that, following transformation, the progeny of the strand elongated in vitro and the template strand have different phenotypes. The method is general and applicable to any DNA segment; the type of base pair substitution and its position can be chosen at will. The combined efficiency of mutagenesis and selection allows for 85% frequency of mutants in all analyzed clones.
Copolymerization of Propylene and Polar Allyl Monomer with Zirconocene/Methylaluminoxane Catalyst:  Catalytic Synthesis of Amino-Terminated Isotactic Polypropylene (2004)

The copolymerizations of propylene with allyl monomers carrying alcohol or amine were conducted by the rac-dimethylsilylbis(1-indenyl)zirconium dichloride/methylaluminoxane catalyst system. The allyl monomers were pretreated by alkylaluminum before the polymerization. Although the efficiency of the introduction of allyl alcohol-based comonomer was extraordinary low (0.04−0.08 mol % in polymer), the trimethylaluminum-treated allylamine was found to be introduced more effectively (0.65 mol % in polymer). The 13C NMR study indicated that the position of allylamine unit in the copolymer was influenced by the kind of alkylaluminum used at the polymerization. In the presence of trimethylaluminum, the terminally aminated polypropylene was obtained ([amino end polymer]/[whole polymer] = 0.83). In the presence of triisobutylaluminum, however, the allylamine unit is located both on the main chain and at the chain end. The end-aminated polymer was probably obtained due to the formation of dormant species after the insertion of the comonomer followed by the chain transfer to alkylaluminum.
Modeling of a Z-IFE Hydrogen Plant Using MELCOR-H2. (2016)

Abstract not provided.
Enhanced Electrical and Thermal Interconnects by the Self-Assembly of Nanoparticle Necks Utilizing Capillary Bridging (2014)

This work presents enhanced composite joints that support both electrical and thermal transport in electronic packages. The joints are sequentially formed by applying a nanoparticle suspension, evaporating a solvent, self-assembling of nanoparticles by capillary bridging, and the formation of so called “necks” between micrometer-sized features. This sequence is used to either form low temperature electrical joints under copper pillars or enhanced percolating thermal underfills (ePTU) with areal contacts between filler particles of the composite. The report discusses processing aspects of the capillary bridges evolution and of uniform neck formation, it discusses strategies to achieve mechanically stable necks, and it compares the performance of the achieved joints against state-of-the-art solutions. The capillary bridge evolution during liquid evaporation was investigated in copper pillar arrays and random particle beds. The vapor–liquid interface first penetrates locations of low pillar or particle density resulting in a dendritic fluid network. Once the network breaks up, individual necks form. For aqueous nanosuspensions, highly uniform necks with high yield were obtained by evaporation at 60 °C. Isothermal conditions were preferred to yield equal neck counts at the cavity's top and bottom surfaces. Mechanically stable silver necks required an annealing at only 150 °C, dielectric necks an annealing at 140 °C with a bimodal approach. Therein polystyrene (PS) nanoparticles occupy interstitial positions in densly packed alumina necks, then melt and adhere to the alumina. The electrical necks showed a shear strength of 16 MPa, equivalent to silver joints used in power electronic packages. The thermal necks yielded a thermal conductivity of up to 3.8 W/mK, five-fold higher than commercially available capillary thermal underfills.
Association of Rare APOE Missense Variants V236E and R251G With Risk of Alzheimer Disease (2022)

In this genetic association study, a novel variant associated with AD was identified: R251G always coinherited with ε4 on the APOE gene, which mitigates the ε4-associated AD risk. The protective effect of the V236E variant, which is always coinherited with ε3 on the APOE gene, was also confirmed. The location of these variants confirms that the carboxyl-terminal portion of apoE plays an important role in AD pathogenesis. The large risk reductions reported here suggest that protein chemistry and functional assays of these variants should be pursued, as they have the potential to guide drug development targeting APOE.