gebhart/patrepeval huggingface dataset:
Sphingosine Kinase 1: A New Modulator of Hypoxia Inducible Factor 1α during Hypoxia in Human Cancer Cells (2008)

Here, we provide the first evidence that sphingosine kinase 1 (SphK1), an oncogenic lipid kinase balancing the intracellular level of key signaling sphingolipids, modulates the transcription factor hypoxia inducible factor 1alpha (HIF-1alpha), master regulator of hypoxia. SphK1 activity is stimulated under low oxygen conditions and regulated by reactive oxygen species. The SphK1-dependent stabilization of HIF-1alpha levels is mediated by the Akt/glycogen synthase kinase-3beta signaling pathway that prevents its von Hippel-Lindau protein-mediated degradation by the proteasome. The pharmacologic and RNA silencing inhibition of SphK1 activity prevents the accumulation of HIF-1alpha and its transcriptional activity in several human cancer cell lineages (prostate, brain, breast, kidney, and lung), suggesting a canonical pathway. Therefore, we propose that SphK1 can act as a master regulator for hypoxia, giving support to its inhibition as a valid strategy to control tumor hypoxia and its molecular consequences.
Sol-gel synthesis of metal nanocomposites (1992)

Gold nano composites with ORMOCER matrices have been prepared by a controlled precipitation process from HAuCl4 solutions, using silanes of the type NH2[(CH2)2NH]x(CH2)3Si(OR)3 (with x=1,2) as a stabilizing agents. In the first step, formation and properties of the ligand stabilized colloidal suspensions was investigated. The different ligands are influencing the adsorption spectra, showing surface plasmon bands between 380 and 700 nm. With the diamo silane, photoreduction is required for the colloid formation, whereas in the case of triamo silane, the colloid formation occurs spontaneously. In the second step, the formation of colloid films on glass substrates was investigated.
Abstract PR15: Primary resistance to MAPK inhibition in BRAF-mutant cancer is determined by kinetics of feedback release (2013)

Abstract Activating mutations in the serine/threonine kinase BRAF are among the most common genetic lesions across all cancers. Despite the clinical success of drugs that either inhibit the oncogene or its downstream effects on the MAPK pathway, only subgroups of BRAF-mutant patients enriched in different tumor lineages will respond to these therapeutics. Here, we show that MAPK-inhibition results in feedback-mediated reactivation of RAS signaling and a resulting paradoxical activation of BRAF/CRAF dimerization. We find, that the formation of paradoxical BRAF/CRAF dimers is dependent on the levels of GTP-RAS whereas transcriptional regulation of ERK signaling modulators as well as activation of receptor tyrosine kinases does not primarily contribute to this process. Using inhibitor-coupled beads, we provide evidence that MAPK inhibition leads to hyperactivation of BRAF and CRAF that results in reactivation of downstream effectors and therefore leads to primary resistance to MAPK inhibition. This feedback-dependent resistance can be suppressed by either genetic depletion of BRAF or the combination of MEK and RAF inhibitors. Overall, our data provide novel insights into the kinetics of primary resistance to MAPK inhibitors in BRAF-mutant tumors and might help to improve the efficacy of targeted therapies in these patients. This abstract is also presented as Poster B03. Citation Format: Martin L. Sos, John D. Gordan, Kevan M. Shokat. Primary resistance to MAPK inhibition in BRAF-mutant cancer is determined by kinetics of feedback release. [abstract]. In: Proceedings of the AACR Precision Medicine Series: Synthetic Lethal Approaches to Cancer Vulnerabilities; May 17-20, 2013; Bellevue, WA. Philadelphia (PA): AACR; Mol Cancer Ther 2013;12(5 Suppl):Abstract nr PR15.
Practical multi-band antenna for 3G and 4G mobile services (2017)

This paper presents a compact antenna design based on PIFA configuration with multi-band features. The present design operates over the lower and upper bands of LTE700/2600MHz, together with the existing standard of GSM1900MHz. An approach of size miniaturization and triple band characteristics was applied through the embedded slot over the radiator surface. The antenna occupies a small volume of 13.5×50×7mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sup> , which is mounted over the ground plane with dimensions of 50×100mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> . Moreover, two identical antennas are integrated within the home device, where their results are checked and compared with the standalone version of the antenna.
A Wearable and Wireless Sensor System for Real-Time Monitoring of Toxic Environmental Volatile Organic Compounds (2009)

An integrated volatile organic toxicants sensor with a Bluetooth device interface has been developed. The device is based on novel tuning fork sensor platform along with a wireless communication/interface technology taken in an integrated system approach. It features high sensitivity and selectivity. The sensitivity and selectivity are accomplished through the use of novel tuning fork sensor modified by design (molecularly imprinted) polymers and selective filtering. Experiments have shown that the device can detect toxic volatile organic compounds (VOCs) under high concentrations of common interferents from flavors and fragrances. Applications of the device for detection of BTEX in real-world situations such as outdoor and gas station VOCs have also been demonstrated. All these features make the device a promising candidate to be deployed in real-world applications, particularly in environmental health and air pollution studies.
Constitutive Hybrid Processes: a Process-Algebraic Semantics for Hybrid Bond Graphs (2008)

Models of physical systems have to be based on physical principles such as conservation of energy and continuity of power. These principles are inherently enforced by the bond graph modeling formalism. Often, however, physical components may be best modeled as piecewise continuous with discrete mode changes, which leads to a violation of continuity principles. To support such hybrid models, bond graphs can be extended by facilitating a dynamic model structure, resulting in hybrid bond graphs. Behavior generation then requires computing continuous-time evolution, detecting the occurrence of events, executing the discrete state changes and re-initializing the continuous-time state. This paper presents a comprehensive representation of these different aspects of behavior using hybrid process algebra. The behavior of a hybrid bond graph can then be studied using a uniform representation while a direct correspondence with the elements of the bond graph is maintained. Additionally, non-determinism can be included in hybrid bond graph semantics which may alleviate the modeling task without being detrimental to the required analyses.
NFC-Capable Mobile Devices for Mobile Payment Services (2007)

An old saying coming from the telecom world states that nothing can be really considered as a service unless you are able to charge for it. As we move towards a service-oriented society, the necessity to pay in real time for a variety of services via different channels anywhere, anytime, in any currency increases. According to Gartner (www.gartner.com), worldwide mobile phone sales totaled 816.6 million units in 2005, a 21% increase from 2004. Due to the high penetration rates of the mobile devices, they pose an interesting candidate for the real-time payment scenarios. Several efforts have already been done (Karnouskos, 2004), but as new technology comes aboard, new capabilities are also brought along. Near Field Communication (NFC) is such a technology, which due to the industry support and its low cost (in comparison with similar ones) may become dominant in short-range communication among a variety of devices, including mobile phones. NFC is well equipped in order to facilitate mobile payments with little interference from the user side.
Anti-VEGF Injections and Glaucoma Surgery (2017)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Ophthalmology HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Ophthalmology journal
Recombinant systems in protein expression : proceedings of the Labsystems Research Symposium II on Recombinant Systems in Protein Expression, Imatra, Finland, 23-26 July 1989 (1990)

PROKARYOTIC SYSTEMS. The pEX vectors and their uses in molecular and cell biology (K.K. Stanley). Way to search for ligands binding to a taxon-specific lens crystallin (G.G. Gause Jr, M.V. Knyazeva, S.M. Dolgilevich and A.S. Mikaelyan). cDNA sequence and expression of cytovillin, a microvillar Mr 75 000 protein (O. Turunen, R. Pakkanen, E.-M. Salonen, T. Wahlstrom and A. Vaheri). pEX-env. A novel expression plasmid coding for a recombinant HIV-1 envelope peptide expression and purification of the recombinant antigen for diagnostic purposes (M.F. Schinkinger, H. Mucke, W.J. Frantsits, T.F. Schulz and M.P. Dierich). LOWER EUKARYOTIC SYSTEMS. Glycosylation and export of heterologous proteins expressed in yeast (V.L. Mackay, C. Yip, S. Welch, T. Gilbert, P. Seidel, F. Grant and P. O'Hara). Yeast Expression: Secretion and intracellular systems (P.J. Barr, O.B. Mason, P.A. Wong, H.L. Gibson, M.C. Kiefer and A.J. Brake). Trichoderma reesei - A host for protein production (A. Harkki, J. Uusitalo, S. Vanhanen, M. Bailey and J. Knowles). Construction of expression vectors based on the yeast MELI gene expression of fungal cellulase in yeast (P.L. Suominen, R. Buhler and P. Liljestrom). Secretion into the medium of hepatitis B surface antigen particles by the methylotropic yeast Hansenula polymopha (S.-H. Shen, L. Bastien and S.N. Slilaty). HIGHER EUKARYOTIC SYSTEMS. Viral vectors - DNA-viruses. The development and use of baculovirus vectors for multigene expression (D.H.L. Bishop, M. Hill-Perkins, K. Takehara, T. Urakawa, U. Weyer and R.D. Possee). Factors limiting biosynthesis and secretion of factor VIII in mammalian cells (R.J. Kaufman, D.D. Pittman, K.A. Marquette, L.C. Wasley and A.J. Dorner). An improved chloramphenicol acetyltransferase expression vector system for mapping transcriptional and post-transcriptional regulatory elements in animal cells (C.C. Baker). Expression of human papillomavirus Type 16 proteins in Escherichia coli and their use as antigens in serological tests (I. Jochmus-Kudielka and L. Gissmann). The production of adenovirus Types 5 and 12 E1A proteins in the baculovirus expression system (D.S. Peeper, A. Zantema, M.H.M. Noteborn and A.J. van der Eb). Construction of an intact equine herpesvirus Type-1 (EHV-1) glycoprotein gene and its expression in COS cells (W.A. Bonass, D.M. Elton, J.-M. Stocks, R.A. Killington, D.M. Meredith and I.W. Halliburton). Polyhedrin promotor-directed expression of rubella virus spike proteins in baculovirus-infected lepidopteran cells (C. Oker-Blom, R.F. Pettersson and M.D. Summers). Immunoglobulin double isotype expression by trans-mRNA in a human MU chain transgenic mouse (A. Shimizu, M.C. Nussenzweig, T.-R. Mizuta, P. Leder and T. Honjo). Expression in animal cells. Multiple regulatory pathways of human HSP70 gene transcription (G.T. Williams, T.K. McClanahan and R.I. Morimoto). Automatic directional cloning: An efficient method to construct eukaryotic expression cDNA libraries (T. Miki, T.
Simultaneous geometry and texture display based on lateral force for touchscreen (2013)

Displaying haptic and tactile information on a touchscreen is one of the key technologies for human interfaces. In this paper we propose a method that allows the user to simultaneously feel both large bump and small textures through a screen. Our method employs lateral force and direction-controlled mechanical vibration (around 0-400 Hz). The technology allows not only geometrical shapes but also textures to be felt. Our experiments revealed that with our proposed method almost all people can simultaneously detect geometry and texture information on a touchscreen. In addition, providing adequate direction for the vibration enhances the feelings of softness.
ChemInform Abstract: Highly Efficient Enantioselective Epoxidation of α,β‐Enones Catalyzed by Cheap Chiral Lanthanum and Gadolinium Alkoxides. (2002)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Scalable Access Control in Content-Based Publish-Subscribe Systems (2006)

Abstract. Content-based publish-subscribe (pub-sub) systems are an emerging paradigm for building a large number of distributed systems. Access control in a pub-sub system refers to secure distribution of events to clients subscribing to those events without revealing its secret attributes to the unauthorized subscribers. To provide confidentiality guarantees the secret attributes in an event is encrypted so that only authorized subscribers can read them. However, in a content-based pub-sub system, every event can potentially have a different set of authorized subscribers. In the worst case, for NS subscribers, there are 2 NS subgroups, and each event can potentially go to a different subgroup. Hence, efficient key management is a big challenge for implementing access control in pub-sub systems. In this paper, we describe efficient and scalable key management algorithms for securely implementing access control rules in pub-sub systems. We ensure that the key management cost is linear in the number of subscriptions and completely independent of the number of subscribers NS. We present a concrete implementation of our proposal on an operational pub-sub system. An experimental evaluation of our prototype shows that our proposal meets the security requirements while maintaining the scalability and performance of the pub-sub system.
625. RNAi Therapy for Dominant Limb Girdle Muscular Dystrophy Type 1A (2016)

Limb Girdle Muscular Dystrophy (LGMD) refers to a group of disorders characterized by progressive wasting and weakness of pelvic and shoulder girdle muscles. Patients commonly require wheelchair assistance, and individuals with some forms of LGMD may have cardiac and respiratory muscle involvement. Modest improvements in a limited set of muscles may dramatically improve patients’ quality of life, but currently no effective treatment exists. There are 8 dominant forms of LGMD (LGMD1) that would benefit from gene therapy strategies to reduce their pathogenic alleles. We previously showed the first RNAi-based pre-clinical treatment for LGMD1A, which is caused by dominant mutations in one allele of the myotilin (MYOT) gene. Our strategy involved delivering MYOT targeted artificial microRNAs (miMYOT) to muscles of the T57I transgenic mouse model of LGMD1A using AAV6 vectors. Both 3 months (short-term) and 9 months (long-term) time points showed significant reduction of mutant myotilin mRNA and soluble protein expression. MYOT gene silencing resulted in histological improvements that were accompanied by significant functional correction including increased muscle weight and improved specific force. While the initial study showed proof-of-principle for this therapy, translation of miMYOT requires additional work. Mouse knockout studies suggested MYOT is a non-essential gene, as animals lacking MYOT develop normally. In the case of LGMD1A this would suggest the mutation is harmful but complete loss would be tolerated. However, we do not currently know if haploinsufficiency of wild-type MYOT is tolerated in other organisms, including humans. To address this question, we delivered morpholinos or AAV.miRNAs to multiple species. We found knockdown of endogenous MYOT to be safe in developing Xenopus, adult mice and non-human primates, suggesting that a non-allele-specific silencing approach for MYOT would be tolerated in LGMD1A-targeted treatments. We also performed dose escalations to assess the safety parameters of AAV.miMYOT vectors and utilized microarray data to identify potential biomarkers for clinical outcome measures. We then validated that some of our lead biomarkers were significantly normalized in miMYOT-treated T57I mice. Together these data provide additional data to support the further translation of miMYOT therapy for LGMD1A.
Risk factors for lower extremity fatigue among assembly plant workers (2010)

While these results identify carpet as being protective against lower extremity fatigue, no similar relationship was identified for anti-fatigue mats. No adverse relationship was found between hard surfaces such as concrete and lower extremity fatigue. Given the high costs associated with work-related fatigue, future areas for potential intervention include smoking cessation, specific shoe recommendations, and enhancing psychosocial aspects of work such as supervisor support.
Effect of La Doping on the Electronic Structure of SrTiO3. (2000)

For undoped-SrTiO3 and La-doped SrTiO3 single crystals, X-ray photoemission spectroscopy (XPS) measurements were performed with monochromated Al Kα radiation, Valence band (VB) spectra of as-fractured SrTiO3 exhibit two peaks, P1 and P2, arising from the O 2pπ and O 2pσ bonding orbitals, respectively. Upon adding 3.0atm% of La to SrTiO3, the valley between P1 and P2 disappears in the VB spectrum. Calculated VB spectra obtained from a molecular orbital calculation reproduce well the experimental XPS spectra for the samples.
Anionic pH Sensitive Lipoplexes (2009)

To provide long circulating nanoparticles able to carry a gene to tumors, we have designed anionic pegylated lipoplexes which are pH sensitive. Anionic pegylated lipoplexes have been prepared from the combined formulation of cationic lipoplexes and pegylated anionic liposomes. The neutralization of the particle surface charge as a function of the pH was monitored by light scattering in order to determine the ratio between anionic and cationic lipids that would give pH sensitive complexes. This ratio has been optimized to form particles sensitive to pH change in the range 5.5-6.5. Compaction of DNA into these newly formed anionic complexes is checked by DNA accessibility to picogreen. The transfection efficiency and pH sensitive property of these formulations has been shown in vitro using bafilomycin, a vacuolar H<sup>+</sup>-ATPase inhibitor.
Positive and negative regulatory elements in the upstream region of the rat Cu/Zn-superoxide dismutase gene (1999)

Cu/Zn-superoxide dismutase (SOD1) catalyses the dismutation of superoxide radicals and neutralizes the oxidative effects of various chemicals. Deletion analysis of the upstream region of the rat SOD1 gene revealed that the promoter contains a positive regulatory element (PRE) and a negative regulatory element (NRE), which encompass the regions from -576 to -412 and from -412 to -305 respectively from the site of initiation of transcription. These DNA elements showed enhancer and silencer activities respectively in the natural context and in a heterologous promoter system. Using an electrophoretic-mobility-shift assay and a supershift assay with a specific antibody, the cis-elements of the PRE and NRE were identified as binding sites for transcription factors Elk1 and YY1 (Ying-Yang 1) respectively. Consistent with the presumed roles of the PRE and NRE, Elk1 increased SOD1 gene transcription about 4–5-fold, whereas YY1 exerted a negative effect of about 6-fold. Mutations of the Elk1- and YY1-binding sites led to diminution and elevation respectively of transcriptional activities, both in the natural context and in heterologous promoter systems. These results suggest that the transcription factors Elk1 and YY1, binding in the PRE and NRE respectively, co-ordinate the expression of the SOD1 gene.
Universal adaptive optics for microscopy through embedded neural network control (2023)

Abstract The resolution and contrast of microscope imaging is often affected by aberrations introduced by imperfect optical systems and inhomogeneous refractive structures in specimens. Adaptive optics (AO) compensates these aberrations and restores diffraction limited performance. A wide range of AO solutions have been introduced, often tailored to a specific microscope type or application. Until now, a universal AO solution – one that can be readily transferred between microscope modalities – has not been deployed. We propose versatile and fast aberration correction using a physics-based machine learning assisted wavefront-sensorless AO control (MLAO) method. Unlike previous ML methods, we used a bespoke neural network (NN) architecture, designed using physical understanding of image formation, that was embedded in the control loop of the microscope. The approach means that not only is the resulting NN orders of magnitude simpler than previous NN methods, but the concept is translatable across microscope modalities. We demonstrated the method on a two-photon, a three-photon and a widefield three-dimensional (3D) structured illumination microscope. Results showed that the method outperformed commonly-used model-based sensorless AO methods. We also showed that our ML-based method was robust in a range of challenging imaging conditions, such as extended 3D sample structures, specimen motion, low signal to noise ratio and activity-induced fluorescence fluctuations. Moreover, as the bespoke architecture encapsulated physical understanding of the imaging process, the internal NN configuration was no-longer a “black box”, but provided physical insights on internal workings, which could influence future designs.
Development and implementation of unique testing methods for the Solar Probe Plus array (2015)

As a “NASA mission to touch the sun” Solar Probe Plus (SPP) reveals challenges previously not encountered in space solar panel design and testing. Simulating flight conditions require new testing methods and equipment which have been specifically developed for this unique PV application. Testing under high intensity, high temperature conditions over large areas poses design and logistic challenges, while testing cell performance degradation to a measurement level error of <;1% involved fabrication of custom LED simulators.