gebhart/patrepeval huggingface dataset:
Tyrosine phosphorylation regulates phospholipase C activation in human T-cells (1991)

Conference Article| April 01 1991 Tyrosine phosphorylation regulates phospholipase C activation in human T-cells Jeffrey A. Ledbetter; Jeffrey A. Ledbetter 1Oncogen Division, Bristol-Myers Squibb Pharmaceutical Research Institute, 3005 First Avenue, Seattle, WA 98121, U.S.A. Search for other works by this author on: This Site PubMed Google Scholar Laura S. Grosmaire; Laura S. Grosmaire 1Oncogen Division, Bristol-Myers Squibb Pharmaceutical Research Institute, 3005 First Avenue, Seattle, WA 98121, U.S.A. Search for other works by this author on: This Site PubMed Google Scholar Theta T. Tsu; Theta T. Tsu 1Oncogen Division, Bristol-Myers Squibb Pharmaceutical Research Institute, 3005 First Avenue, Seattle, WA 98121, U.S.A. Search for other works by this author on: This Site PubMed Google Scholar Gary L. Schieven Gary L. Schieven 1Oncogen Division, Bristol-Myers Squibb Pharmaceutical Research Institute, 3005 First Avenue, Seattle, WA 98121, U.S.A. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1991) 19 (2): 269–272. https://doi.org/10.1042/bst0190269 Article history Received: December 31 1990 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation Jeffrey A. Ledbetter, Laura S. Grosmaire, Theta T. Tsu, Gary L. Schieven; Tyrosine phosphorylation regulates phospholipase C activation in human T-cells. Biochem Soc Trans 1 April 1991; 19 (2): 269–272. doi: https://doi.org/10.1042/bst0190269 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: PLC, phospholipase C, mAbs, monoclonal antibodies, IL-2, interleukin-2 This content is only available as a PDF. © 1991 Biochemical Society1991 Article PDF first page preview Close Modal You do not currently have access to this content.
Inhibition of in vitro proliferative responses of human lymphocytes by rimantadine hydrochloride (1979)

Rimantadine hydrochloride (alpha-methyl-1-adamantanemethylamine hydrochloride) inhibits the in vitro proliferative response of human peripheral blood lymphocytes to mitogenic and antigenic stimulation. Addition of drug (25 micrograms/ml) at the initiation of 5-day cultures suppressed the responses to phytohemagglutinin, pokeweed mitogen, and concanavalin A by 25, 65, and 90%, respectively. Similarly, responses to streptokinase-streptodornase, tetanus toxoid, and A2/Aichi influenza vaccine were significantly inhibited at concentrations as low as 10 micrograms of rimantadine per ml. Viability studies on 5-day cultures using trypan blue exclusion showed no significant difference between drug-treated and untreated controls. Furthermore, addition of drug on day 3 of 8-day cultures, at a time when the majority of cells had undergone blastogenesis, greatly suppressed the responses to these mitogens. These studies suggest that, in addition to its antiviral action, rimantadine interferes with the generation of cellular immune responses.
Labeling of benzodioxin piperazines with fluorine‐18 as prospective radioligands for selective imaging of dopamine D4 receptors (2013)

The D(4) receptor is of high interest for research and clinical application but puts high demands on appropriate radioligands to be useful tools for investigation. Search for adequate radioligands suitable for in vivo imaging is therefore still in progress. The potential neuroleptic drug 6-(4-[4-fluorobenzyl]piperazin-1-yl)benzodioxin shows high affinity and selectivity to the D(4) receptor. Derivatization of this lead structure by adding hydrophilic moieties was carried out in order to lower its lipophilicity what led to three new putative dopamine receptor D(4) ligands. A comprehensive description of the syntheses of standard compounds and corresponding labeling precursors is given which were obtained in satisfactory yields. Furthermore, the radiosyntheses by direct (18) F-labeling and build-up synthesis were compared. All derivatives of 6-(4-[4-fluorobenzyl]-piperazin-1-yl)benzodioxin were successfully synthesized in (18) F-labeled form with radiochemical yields of 9-35% and molar activities of 30-60 GBq/µmol using one-pot procedures.
Integrated Analyses of Data from Clinical Trials of Delandistrogene Moxeparvovec (SRP-9001) in Duchenne Muscular Dystrophy (DMD) (S48.006) (2023)

To evaluate functional data in patients with Duchenne muscular dystrophy (DMD) ≥4 to ≤8 years of age at 1 year following a single intravenous (IV) infusion of delandistrogene moxeparvovec (SRP-9001) versus a propensity-score-weighted external control (EC) cohort.
CD74 is a regulator of hematopoietic stem cell maintenance (2021)

Hematopoietic stem and progenitor cells (HSPCs) are a small population of undifferentiated cells that have the capacity for self-renewal and differentiate into all blood cell lineages. These cells are the most useful cells for clinical transplantations and for regenerative medicine. So far, it has not been possible to expand adult hematopoietic stem cells (HSCs) without losing their self-renewal properties. CD74 is a cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF), and its mRNA is known to be expressed in HSCs. Here, we demonstrate that mice lacking CD74 exhibit an accumulation of HSCs in the bone marrow (BM) due to their increased potential to repopulate and compete for BM niches. Our results suggest that CD74 regulates the maintenance of the HSCs and CD18 expression. Its absence leads to induced survival of these cells and accumulation of quiescent and proliferating cells. Furthermore, in in vitro experiments, blocking of CD74 elevated the numbers of HSPCs. Thus, we suggest that blocking CD74 could lead to improved clinical insight into BM transplant protocols, enabling improved engraftment.
Acute Effect of Breathing Exercises on Heart Rate Variability in Type 2 Diabetes: A Pilot Study (2014)

In summary, neither UNB nor PB had an impact upon HRV in a healthy older population and had a minimal impact in T2D.
Larrabee (2008)

This paper presents a many-core visual computing architecture code named Larrabee, a new software rendering pipeline, a manycore programming model, and performance analysis for several applications. Larrabee uses multiple in-order x86 CPU cores that are augmented by a wide vector processor unit, as well as some fixed function logic blocks. This provides dramatically higher performance per watt and per unit of area than out-of-order CPUs on highly parallel workloads. It also greatly increases the flexibility and programmability of the architecture as compared to standard GPUs. A coherent on-die 2nd level cache allows efficient inter-processor communication and high-bandwidth local data access by CPU cores. Task scheduling is performed entirely with software in Larrabee, rather than in fixed function logic. The customizable software graphics rendering pipeline for this architecture uses binning in order to reduce required memory bandwidth, minimize lock contention, and increase opportunities for parallelism relative to standard GPUs. The Larrabee native programming model supports a variety of highly parallel applications that use irregular data structures. Performance analysis on those applications demonstrates Larrabee's potential for a broad range of parallel computation.
A hierarchical system structure for coordinated control of industrial manipulators (2005)

This paper describes a framework for the intelligent control of industrial manipulators. The structure is designed to allow a robotic system to take full advantage of the improved sensing capabilities and more powerful languages that are now becoming available. The framework consists of two layers of hierarchy for the coordinated control of multi-jointed industrial manipulators. The low level control mechanism, responsible for servoing each joint, is adaptive to both manipulator dynamics and payload changes. The individual low-level joint controllers are integrated and coordinated by the high level control structure. Together, the two levels provide a versatile basis for manipulator control. This basis forms a complete structure, and is suited for interfacing with yet higher level intelligent systems such as task planners, high level languages, and environment sensitive path planners.
Selective Corrosion of Stainless Steel at Low pH (2013)

This chapter contains sections titled: Introduction Experimental Methods Results Discussion Conclusions
Faculty Opinions recommendation of Age influences DNA methylation and gene expression of COX7A1 in human skeletal muscle. (2008)

AIMS/HYPOTHESIS: Reduced oxidative capacity of the mitochondria in skeletal muscle has been suggested to contribute to insulin resistance and type 2 diabetes. Moreover, a set of genes influencing oxidative phosphorylation (OXPHOS) is downregulated in diabetic muscle. Here we studied whether genetic, epigenetic and non-genetic factors influence a component of the respiratory chain, COX7A1, previously shown to be downregulated in skeletal muscle from patients with type 2 diabetes. The specific aims were to: (1) evaluate the impact of genetic (single nucleotide polymorphisms [SNPs]), epigenetic (DNA methylation) and non-genetic (age) factors on the expression of COX7A1 in human skeletal muscle; and (2) investigate whether common variants in the COX7A1 gene are associated with increased risk of type 2 diabetes.METHODS: COX7A1 mRNA expression was analysed in muscle biopsies from young (n = 110) and elderly (n = 86) non-diabetic twins and related to measures of in vivo metabolism. Genetic variants (three SNPs) from the COX7A1 locus were genotyped in the twins and in two independent type 2 diabetes case-control cohorts (n = 1466 and 6380, respectively). DNA methylation of the COX7A1 promoter was analysed in a subset of twins (ten young, ten elderly) using bisulphite sequencing.RESULTS: While DNA methylation of the COX7A1 promoter was increased in muscle from elderly compared with young twins (19.9 +/- 8.3% vs 1.8 +/- 2.7%; p = 0.035), the opposite was found for COX7A1 mRNA expression (elderly 1.00 +/- 0.05 vs young 1.68 +/- 0.06; p = 0.0005). The heritability of COX7A1 expression was estimated to be 50% in young and 72% in elderly twins. One of the polymorphisms investigated, rs753420, influenced basal COX7A1 expression in muscle of young (p = 0.0001) but not of elderly twins. The transcript level of COX7A1 was associated with increased in vivo glucose uptake and VO(2max) (p = 0.009 and p = 0.001, respectively). We did not observe any genetic association between COX7A1 polymorphisms and type 2 diabetes after correcting for multiple testing.CONCLUSIONS/INTERPRETATION: Our results provide further evidence for age as a factor influencing DNA methylation and expression of OXPHOS genes, and thereby in vivo metabolism. PMID: 18488190
Short-Term Kinetics of Tumor Antigen Expression in Response to Vaccination (2001)

The melanoma patient's immune response to tumor has been extensively studied. Yet, the frequently observed coexistence of tumor-associated Ag (TAA)-specific T cells with their target cells in vivo remains unexplained. Loss of TAA expression might contribute to this paradox. We studied TAA expression in metastases by obtaining fine-needle aspirations from 52 tumor lesions in 30 patients with melanoma before and soon after immunotherapy. Limitations due to low amounts of starting material were overcome with a high fidelity antisense RNA amplification method. TAA expression was measured by quantitative real-time PCR of anti-sense RNA. Decrease in gp100/Pmel-17 TAA preceded tumor disappearance in several instances and could be best explained by immune selection because most patients had received gp100/Pmel-17-specific vaccination. Conversely, immune selection was absent in nonregressing lesions. These observations suggest that vaccination, when successful, triggers a broad inflammatory reaction that can lead to tumor destruction despite immune selection. Additionally, lack of clinical response might be attributed to lack of this initiating event rather than immune escape. This study provides an insight into the natural history of tumors and defines a strategy for the characterization of gene expression in tumors during therapy.
Programmable spatially variant multiresolution readout capability on a sensor focal plane (2002)

This paper presents a new image sensor with programmable multiresolution readout capability. The proposed image sensor can output data at varying resolutions. Differing to our previously reported spatially variant sampling sensor, which sub-samples without filtering, in the multiresolution sensor blocks of the pixels are averaged and read out so the new sensor does not suffer from aliasing effects. The resolution can be controlled by varying the block size. We made a prototype of 64/spl times/64 pixels.
Piezoelectric Ink‐Jet Printing of Horseradish Peroxidase: Effect of Ink Viscosity Modifiers on Activity (2007)

Abstract In this study a bioink containing the horseradish peroxidase (HRP) enzyme was formulated and jetted with a piezoelectric‐based ink‐jet deposition system. A number of polymers with different molecular weights, functional groups and electric charge were tested as viscosity modifiers. The impact of viscosity modifiers on HRP activity was systematically studied. It was found that viscosity modifiers typically used in commercial ink formulations significantly impaired HRP activity possibly due to limitations in diffusion. In contrast, carboxymethyl cellulose increased viscosity to the desired range for piezoelectric jetting without negatively affecting the HRP activity. Good jettability was obtained for the HRP bioink by adding a suitable surfactant, a viscosity modifier and a humectant. magnified image
4D CT amplitude binning for the generation of a time-averaged 3D mid-position CT scan (2014)

The purpose of this study was to develop a method to use amplitude binned 4D-CT (A-4D-CT) data for the construction of mid-position CT data and to compare the results with data created from phase-binned 4D-CT (P-4D-CT) data. For the latter purpose we have developed two measures which describe the regularity of the 4D data and we have tried to correlate these measures with the regularity of the external respiration signal. 4D-CT data was acquired for 27 patients on a combined PET-CT scanner. The 4D data were reconstructed twice, using phase and amplitude binning. The 4D frames of each dataset were registered using a quadrature-based optical flow method. After registration the deformation vector field was repositioned to the mid-position. Since amplitude-binned 4D data does not provide temporal information, we corrected the mid-position for the occupancy of the bins. We quantified the differences between the two mid-position datasets in terms of tumour offset and amplitude differences. Furthermore, we measured the standard deviation of the image intensity over the respiration after registration (σregistration) and the regularity of the deformation vector field (Delta J) to quantify the quality of the 4D-CT data. These measures were correlated to the regularity of the external respiration signal (σsignal).The two irregularity measures, Delta J and σregistration, were dependent on each other (p<0.0001, R2=0.80 for P-4D-CT, R2=0.74 for A-4D-CT). For all datasets amplitude binning resulted in lower Delta J and σregistration and large decreases led to visible quality improvements in the mid-position data. The quantity of artefact decrease was correlated to the irregularity of the external respiratory signal.The average tumour offset between the phase and amplitude binned mid-position without occupancy correction was 0.42 mm in the caudal direction (10.6% of the amplitude). After correction this was reduced to 0.16 mm in caudal direction (4.1% of the amplitude). Similar relative offsets were found at the diaphragm. We have devised a method to use amplitude binned 4D-CT to construct motion model and generate a mid-position planning CT for radiotherapy treatment purposes. We have decimated the systematic offset of this mid-position model with a motion model derived from P-4D-CT. We found that the A-4D-CT led to a decrease of local artefacts and that this decrease was correlated to the irregularity of the external respiration signal.
Delivery agents that facilitate the absorption of macromolecular drugs. (1999)

Macromolecules comprise a growing group of new therapeutics with great clinical promise. To date, these drugs are limited in their application because they are effective only when administered parenterally. Unfortunately, however, macromolecular drugs are not absorbed following nonparenteral dosing because the human body is designed to exclude them. To overcome the numerous obstacles to the noninvasive delivery of these drugs, various approaches are under investigation including the use of delivery agents to promote drug absorption. This review provides a summary of the novel approaches currently in progress in the areas of transdermal, transmucosal and oral delivery of macromolecular drugs facilitated by delivery agents.
Antigenic and structural characterization of Treponema pallidum (Nichols strain) endoflagella (1988)

Purified endoflagella from Treponema pallidum, Nichols strain, were characterized both structurally and antigenically. Structural analysis showed T. pallidum endoflagella are composed of 35- and 33-kilodalton (kDa) subunits which lack cysteine and do not share N-terminal amino acid sequence homology (20 residues). Intact endoflagella were dissociated into the composite subunits by incubation, which disrupts noncovalent bonds. Antiserum raised against purified T. pallidum endoflagella identified shared epitopes on the endoflagellar polypeptides of the nonpathogen, Treponema phagedenis biotype Reiter. Pathogen-specific epitopes were also found on the 35- and 33-kDa polypeptides by using affinity-purified endoflagellar antibodies. The pathogen-specific epitopes were localized by immunoblotting analysis of chymotryptic digests of the endoflagellar subunits; 18- and 26-kDa fragments derived from the 35-kDa subunit were found to possess a majority of the pathogen-specific epitopes. Both the 35- and 33-kDa subunits had surface exposure, as determined by immunoelectron microscopy, although additional immunochemical data indicated that the surface exposure of the 35-kDa subunit was greater.
Domain-Centric Design Education (2005)

Industry and students clamor for increased training related to engineering design and analysis methods. Often, students are hired based on the suite of software packages and Design for Manufacturing methods with which they are familiar as opposed to their knowledge of the fundamentals and their ability to formalize and solve open ended design problems. In support of a domain-centric design education, some of the trends that are driving the increased domain emphasis in design education are documented including decreasing computing cost, decreasing communication costs, and decreasing engineering tenures. The paper then discusses the ramifications of these trends on design education and design practice as measurable by the availability of the mechanical engineering design curricula, design publications, and design patents. Finally, a domain-centric design education paradigm is proposed that suggests the differentiation of Mechanical Engineering programs through the replacement of traditional machine and product design courses with domain-specific courses with increased technical specialty.Copyright © 2005 by ASME
Inflammatory Myelopathy Presenting as a Cystic Intramedullary Spinal Cord Lesion (1984)

A case of subacute progressive spinal tetraparesis had myelographic evidence of cervical spinal cord swelling and a delayed metrizamide computed tomographic myelogram (MCTM) suggested cavitation within the swollen spinal cord. Surgical exploration of the cervical cord revealed inflammatory changes only. No syrinx or intramedullary tumour was found. The accumulation of metrizamide within the spinal cord, as demonstrated by MCTM, did not represent a syrinx or a cystic tumour, but more likely an area of inflammation. Because inflammatory myelopathy may simulate an intramedullary tumor or syrinx, careful analysis of all clinical and radiological information is necessary to help make a correct diagnosis.
In-vivo proton MR spectroscopic imaging of the human brain gliomas at 9.4 Tesla: evaluation of metabolite coordinates (2015)

Recently it was demonstrated that the advantages of ultra-high field MR spectroscopic imaging (MRSI), namely the better signal-to-noise ratio and the improved spectral resolution, can be useful in clinical applications. Studies conducted at lower field strengths (below 3T) have shown that an evaluation with the Orthonormal Discriminant Vector method (ODV) enables differentiation between low (WHO grade II and III) and high grade (WHO grade IV) human brain tumors. The aim of this study was to verify the usefulness of the ODV method in assessing human brain tumor spectra measured with MRSI at a field strength of 9.4T.
Quality Evaluation via PPG on the AVFs of Hemodialysis Patients Based on Both Blood Flow Volume and Degree of Stenosis (2019)

The classifier of support vector machine (SVM) learning for assessing quality of arteriovenous fistula (AVF) at hemodialysis (HD) patients using a new photoplethysmography (PPG) sensor are presented in this work. Based on current medical standard, there are two important indices for assessing AVF quality, the blood flow volume (BFV) and the degree of stenosis (DOS). In current clinical practice, BFV and DOS of AVFs are assessed by using an ultrasound Doppler machine, which is bulky, expensive, hard-to-use and time-consuming. Therefore, a new PPG sensor module is designed to provide patients and doctors an inexpensive and small-sized solution to assess AVF quality. The readout of the sensor is successfully optimized to increase the signal to noise ratio (SNR) and reduce the environment interference, the readout circuitries are designed to fit the full dynamic range of analog-digital converter (ADC) and to filter out the noise. To assess quality of AVF, three different machine learning classifiers are developed, where the input features are selected based on optical Beer Lambert's law and hemodynamic model. Finally, the clinical experiment results show that the proposed PPG sensor successfully achieves an accuracy of 87.838% in assessing AVF quality based on satisfactory DOS and BFV measured.
Improving the Accuracy of Predictive Models for Outcomes of Antidepressants by Using an Ontological Adjustment Approach (2022)

For patients with rare comorbidities, there are insufficient observations to accurately estimate the effectiveness of treatment. At the same time, all diagnosis, including rare diagnosis, are part of the International Classification of Disease (ICD). Grouping ICD into broader concepts (i.e., ontology adjustment) can not only increase accuracy of estimating antidepressant effectiveness for patients with rare conditions but also prevent overfitting in big data analysis. In this study, 3,678,082 depressed patients treated with antidepressants were obtained from OptumLabs® Data Warehouse (OLDW). For rare diagnoses, adjustments were made by using the likelihood ratio of the immediate broader concept in the ICD hierarchies. The accuracy of models in training (90%) and test (10%) sets was examined using the area under the receiver operating curves (AROC). The gap in training and test AROC shows how much random noise was modeled. If the gap is large, then the parameters of the model, including the reported effectiveness of the antidepressant for patients with rare conditions, are suspect. There was, on average, a 9.0% reduction in the AROC gap after using the ontological adjustment. Therefore, ontology adjustment can reduce model overfitting, leading to better parameter estimates from the training set.