gebhart/patrepeval huggingface dataset:
Carotid Body Denervation Attenuates Sympathetic Nerve Activity, Shifts the Pressure‐Diuresis Curve to the Left and Improves Survival in rats with Hypertensive Heart Failure (2015)

BACKGROUND Since carotid body denervation (CBD) is known to inhibit excessive sympathetic nerve activity (SNA) in animal models of hypertension and/or heart failure, we hypothesized that CBD improves survival in rats with hypertensive heart failure. METHODS We randomly allocated Dahl salt-sensitive rats fed with high-salt diet (8% NaCl) into 2 groups, CBD (N=20) and SHAM (N=29). At 7 weeks of age, we performed CBD. We assessed echocardiograms, 24-hour urinary norepinephrine (uNE) excretion as a parameter of SNA and arterial pressure (AP). In some rats, we examined the relationship between urinary sodium excretion and AP with or without high-salt diet (the pressure-diuresis curve) at 14 weeks. Finally, we compared survival rate between 2 groups until 16 weeks. RESULTS CBD significantly reduced uNE excretion and shifted the pressure-diuresis curve to the left at 14 weeks (figure A, B). CBD significantly preserved left ventricular ejection fraction (58.5 ± 11 vs. 67.2 ± 8.7%, p<0.05, 2D echo) and reduced biventricular weight (4.73± 0.50 vs. 4.31± 0.46 g/kg, p<0.05) as well as lung weight (5.91 ± 2.5 vs. 4.58 ± 1.0 g/kg, p<0.05) at 16 weeks. Furthermore, CBD markedly improved the survival rate with 91% relative risk reduction (figure C). Postmortem studies indicated that those rats died of heart failure. CONCLUSION CBD significantly inhibits SNA, reduces AP with leftward shifting of pressure-diuresis curve and improves survival in Dahl salt-sensitive rats.
Survival after surgical resection of pulmonary metastases and second primary squamous cell lung carcinomas in head and neck cancer (2008)

Survival after resection of metachronic lung lesions following curative treatment of HNSSC is similar for lesions characterized as second primary squamous cell lung cancer and those characterized as metastases.
TSHZ2 is an EGF-regulated tumor suppressor that binds to the cytokinesis regulator PRC1 and inhibits metastasis (2021)

Unlike early transcriptional responses to mitogens, later events are less well-characterized. Here, we identified delayed down-regulated genes (DDGs) in mammary cells after prolonged treatment with epidermal growth factor (EGF). The expression of these DDGs was low in mammary tumors and correlated with prognosis. The proteins encoded by several DDGs directly bind to and inactivate oncoproteins and might therefore act as tumor suppressors. The transcription factor teashirt zinc finger homeobox 2 (TSHZ2) is encoded by a DDG, and we found that overexpression of TSHZ2 inhibited tumor growth and metastasis and accelerated mammary gland development in mice. Although the gene <i>TSHZ2</i> localizes to a locus (20q13.2) that is frequently amplified in breast cancer, we found that hypermethylation of its promoter correlated with down-regulation of <i>TSHZ2</i> expression in patients. Yeast two-hybrid screens and protein-fragment complementation assays in mammalian cells indicated that TSHZ2 nucleated a multiprotein complex containing PRC1/Ase1, cyclin B1, and additional proteins that regulate cytokinesis. TSHZ2 increased the inhibitory phosphorylation of PRC1, a key driver of mitosis, mediated by cyclin-dependent kinases. Furthermore, similar to the tumor suppressive transcription factor p53, TSHZ2 inhibited transcription from the <i>PRC1</i> promoter. By recognizing DDGs as a distinct group in the transcriptional response to EGF, our findings uncover a group of tumor suppressors and reveal a role for TSHZ2 in cell cycle regulation.
Commonsense-Focused Dialogues for Response Generation: An Empirical Study (2021)

Pei Zhou, Karthik Gopalakrishnan, Behnam Hedayatnia, Seokhwan Kim, Jay Pujara, Xiang Ren, Yang Liu, Dilek Hakkani-Tur. Proceedings of the 22nd Annual Meeting of the Special Interest Group on Discourse and Dialogue. 2021.
Chemical ordering dependence of interlayer exchange coupling in Co-Mn-Si/Cr/Co-Mn-Si trilayer structures (2010)

The effect of chemical ordering of a half-metallic full-Heusler alloy ${\text{Co}}_{2}\text{MnSi}$ (CMS) on interlayer exchange coupling (IEC) behavior was investigated in Co-Mn-Si(20 nm)/Cr(1.2 nm)/Co-Mn-Si(7 nm) trilayers. Two types of experimental investigations were employed, namely, the variations in IEC with changing the annealing temperature of the bottom CMS from room temperature to $500\text{ }\ifmmode^\circ\else\textdegree\fi{}\text{C}$ and with changing the Co concentration in top and bottom Co-Mn-Si layers. The $90\ifmmode^\circ\else\textdegree\fi{}$ coupling parameter ${J}_{2}$ and the bilinear coupling parameter ${J}_{1}$ were estimated from the numerical simulations of magnetization curves. Only the strong $90\ifmmode^\circ\else\textdegree\fi{}$ coupling with almost the same strength was observed in both the completely $B2$ ordered and in a mixture of ordered $L{2}_{1}$ and $B2$ structures. The degree of $B2$ ordering as well as the strength of $90\ifmmode^\circ\else\textdegree\fi{}$ coupling was observed to decrease simultaneously with the Co concentration in Co-Mn-Si and $180\ifmmode^\circ\else\textdegree\fi{}$ coupling appeared in highly Co antisite defective $(A2)$ structures, i.e., for the Co concentration over $73\text{ }\text{at}\text{.}\text{ }\mathrm{%}$. This observation showed a clear relationship between the degree of $B2$ ordering and the IEC behavior in full-Heusler Co-Mn-Si-based structures.
Tunable floating active inductor with internal offset reduction (2012)

Presented is a transistor-level implementation of a floating and tunable CMOS active inductor. It is based on the classical gyrator-C topology and is enhanced by adding an internal offset reduction mechanism to guarantee functionality also for unbalanced DC conditions. The realised inductance can be programmed for values between 685 µH and 12.4 mH and is designed to be implemented using standard CMOS technology. Its range of operation is from 250 to 750 kHz and the inductor consumption does not exceed 2 mW.
A Prediction Model for Implementing DVS in Single-Rail Bundled-Data Handshake-Free Asynchronous Circuits (2019)

This paper explores the use of dynamic voltage scaling (DVS) for a particular type of asynchronous circuits, namely the single-rail bundled-data handshake-free asynchronous circuits. With respect to their synchronous counterparts, applying DVS to the targeted type of circuits imposes additional timing constraints that must be met to ensure correct operation. A new model defining these additional constraints is proposed. Such DVS related constraints were never explicitly formulated. The proposed model considers setup and hold timing constraints and transition degradation to ensure proper timing closure. Reported simulation results show that the timing constraints can be satisfied while applying DVS, but also that adjustments might be required to ensure proper pulse propagation and efficient operations.
Quantification of Infection by Alternaria cassiae Using Leaf Immuno‐Autoradiography and Radioimmunosorbent Assays (1993)

Abstract The severity of fungal infection is usually estimated visually as a rating of injury. A more quantitative method was needed to ascertain the effect of additives used to enhance the virulence of mycoherbicidal preparations. A sensitive and accurate serological method is presented for quantitative measurement of Alternaria cassiae infection of Cassia obtusifolia . An antiserum was prepared against a homogenate of mycelium of A. cassiae . A fast and simple procedure of leaf immuno‐autoradiography was developed for the visualization of A. cassiae mycelium on inoculated leaves. A radioimmunosorbent assay (RISA) was developed for quantification of the extent of fungal infection. As little as 1. 6 ng/ ml dry weight equivalent of mycelium could be detected by RISA. There was a linear relationship between the logarithm of the RISA values and the logarithm of fungus concentration up to 300 ng/ml. Reduced sensitivity of the assay was pronounced in extracts containing 400 μ g/ml or more fresh weight of leaves. There was 30–40 % cross‐reactivity of the antiserum with two other species of Alternaria, as well as with Monilinia fructicola , and almost no reactivity was found with three other fungi tested.
Importance of sunscreen products spreading protocol and substrate roughness for in vitro sun protection factor assessment (2009)

The purpose of this study was to evaluate the impact of substrate roughness and of product spreading method on in vitro sun protection factor (SPF) measurement and to define the experimental conditions most appropriate to reach the best level of correlation to in vivo SPF. In vitro SPF assessment was carried out on 13 products (including different formulation types with SPF from 20 to 75) using various in vitro SPF protocols and comparing related predictive potential regarding in vivo SPF. In the first part, two spreading methods were compared on two types of PMMA (Polymethyl methacrylate plate with different roughness. The impact of a second spreading step after product drying was also evaluated. From the various investigated parameters, it was shown that (i) a higher roughness (Ra = 4, 5 microm) was preferred for O/W formulations (ii) using a defined sequence of light linear and circular strokes was more adequate than monitoring product spreading in terms of time and pressure (iii) both correlation to in vivo SPF and results variability were improved when a second spreading step was added. The altered protocol showed a good predictive potential regarding in vivo SPF values for O/W formulations (correlation coefficient 0.92, correlation curve slope 0.98) and coefficient of variation of in vitro results (14% of the mean SPF value) close to what is usually obtained in vivo. The repeatability of the protocol was also demonstrated. In the second part, we evaluated the impact of PMMA plate pre-treatment with paraffinum liquidum before spreading the product to get a better correlation between in vivo and in vitro SPF values for W/O formulations. This allowed us to define a protocol suitable for both O/W and W/O formulations.
Hamiltonian-Driven Adaptive Dynamic Programming for Continuous Nonlinear Dynamical Systems (2017)

This paper presents a Hamiltonian-driven framework of adaptive dynamic programming (ADP) for continuous time nonlinear systems, which consists of evaluation of an admissible control, comparison between two different admissible policies with respect to the corresponding the performance function, and the performance improvement of an admissible control. It is showed that the Hamiltonian can serve as the temporal difference for continuous-time systems. In the Hamiltonian-driven ADP, the critic network is trained to output the value gradient. Then, the inner product between the critic and the system dynamics produces the value derivative. Under some conditions, the minimization of the Hamiltonian functional is equivalent to the value function approximation. An iterative algorithm starting from an arbitrary admissible control is presented for the optimal control approximation with its convergence proof. The implementation is accomplished by a neural network approximation. Two simulation studies demonstrate the effectiveness of Hamiltonian-driven ADP.
A blood sampling microsystem for pharmacokinetic applications: Design, fabrication, and initial results (2009)

This paper describes a microsystem for automated blood sampling from laboratory mice used in pharmacokinetic studies. Intended to be mounted as a "backpack" on a mouse, it uses a microneedle, reservoir, and an actuator to instantaneously prick the animal for a time-point sample, eliminating the need for a tethered catheter with large dead volume. The blood is collected by capillary effect through a 31-33 gauge microneedle (250-210 microm OD) into a approximately 1 microL micromachined steel reservoir. The voice coil actuator provides a peak force of approximately 300 mN, which amply exceeds the measured piercing force of mouse skin (i.e., 60-85 mN for a 31-gauge needle with 12 degrees bevel). The sampling system was tested in vitro using a mock vessel with adjustable pressure; the reservoir was filled in <0.15 s by a combination of the capillary effect and blood pressure. The system may also be used to sample interstitial fluid, but the absence of blood pressure makes it necessary to enhance the capillary effect of the needle. This is accomplished by either electropolishing the inner surface to make it more hydrophilic or using a polymer wire insert to increase the surface area. The steel surface of the reservoir is also coated with silicon oxynitride by plasma-enhanced chemical vapor deposition to improve its hydrophilicity. Blood from fresh bovine tissue was collected into the reservoir to simulate interstitial fluid sampling. In vivo tests on live, anesthetized mice resulted in successful collection of blood into the reservoir. The possible integration of the device in microanalytical systems and the device scalability for multisampling are discussed.
Development of co-evaporated In2S3 buffer layer for Cu2ZnSnSe4 thin film solar cells (2015)

In this work, we focus on the replacement of the commonly used but toxic Cd-based buffer layer by In2S3 thin films deposited by co-evaporation for application in Cu2ZnSnSe4 (CZTSe) solar cells. The impact of the deposition conditions of the buffer layer on the electrical behavior of CZTSe/In2S3 devices is first investigated. The best solar cell efficiencies were obtained for relatively thick In2S3 buffer layers (∼100 nm) deposited at low temperature (<100 °C). It is also observed that low [Cu]/([Zn]+[Sn]) ratio (CZT∼0.75) in the kesterite absorber leads to high efficiency for In-based buffered CZTSe solar cells, while the effect of the CZT ratio on CZTSe/CdS solar cell performances is not so clear. A conversion efficiency of 5.7 % on CZTSe/In2S3 thin film solar cell is achieved.
Maximizing opto‐mechanical interaction using topology optimization (2011)

Abstract This paper studies topology optimization of a coupled opto‐mechanical problem with the goal of finding the material layout which maximizes the optical modulation, i.e. the difference between the optical response for the mechanically deformed and undeformed configuration. The optimization is performed on a periodic cell and the periodic modeling of the optical and mechanical fields have been carried out using transverse electric Bloch waves and homogenization theory in a plane stress setting, respectively. Two coupling effects are included being the photoelastic effect and the geometric effect caused by the mechanical deformation. For the studied objective and material choice it is concluded that the photoelastic effect and the geometric effect counteract each other, which yields designs which are fundamentally different if the optimization takes only one effect into account. When both effects are active a compromise is found; however, a strong regularization is needed in order to achieve reasonable 0–1 designs with a clear physical interpretation. Copyright © 2011 John Wiley &amp; Sons, Ltd.
Nucleophilic Addition to Acetylenes in Superbasic Catalytic Systems. Part 14. Vinylation of Diols in a System CsF—NaOH. (2005)

Abstract For Abstract see ChemInform Abstract in Full Text.
Fluid flow analysis in microfluidic devices by spectral-domain optical Doppler tomography (2005)

Microfluidic devices are becoming increasingly popular for many applications, enabling biological and chemical reactions to be performed with nano- and picoliter sample volumes. Accurate measurement and monitoring of fluid flow behavior in the small channels of microfluidic systems is important for evaluating the performance of existing devices, and in the modeling and design of new microfluidic networks. We present here the results of experiments using spectral-domain optical Doppler tomography (SD-ODT) to measure fluid flow in single-layer microfluidic devices. The principles behind flow imaging with SD-ODT are reviewed, a method for velocity calibration is described, and cross-sectional and en-face images of fluid velocity in microfluidic channels are presented.
Two-photon phosphorescence lifetime microscopy (2PLM) of Oxygen (Conference Presentation) (2020)

Molecular oxygen plays a unique role in cellular energy metabolism by serving as the terminal electron acceptor in the mitochondrial respiratory chain. Quantitative imaging of oxygen can provide invaluable information about metabolism in normal and diseased states. Two-photon phosphorescence lifetime microscopy (2PLM) enables imaging of oxygen concentration gradients in 3D with micron-scale resolution. The method has found use in neuroscience, stem cell and tumor biology. We will discuss the principles of 2PLM and focus on the new phosphorescent probes, which enable imaging of oxygen simultaneously with imaging of temperature.
Development of organic recording media for blue-high NA optical disc system (2003)

We have developed an organic write once media suitable for the DVR-Blue format. By use of organic material with high initial refractive index at 404 rim wavelength, a high density recording of 21 GB per 12 cm diameter single side disc was optimised to a practical level. Further optimisation of the recording pulse strategy and the media in near future promises the achievement of full compatibility with the DVR-Blue format.
CSMA/CN: Carrier Sense Multiple Access With Collision Notification (2011)

A wireless transmitter learns of a packet loss and infers collision only after completing the entire transmission. If the transmitter could detect the collision early [such as with carrier sense multiple access with collision detection (CSMA/CD) in wired networks], it could immediately abort its transmission, freeing the channel for useful communication. There are two main hurdles to realize CSMA/CD in wireless networks. First, a wireless transmitter cannot simultaneously transmit and listen for a collision. Second, any channel activity around the transmitter may not be an indicator of collision at the receiver. This paper attempts to approximate CSMA/CD in wireless networks with a novel scheme called CSMA/CN (collision notification). Under CSMA/CN, the receiver uses PHY-layer information to detect a collision and immediately notifies the transmitter. The collision notification consists of a unique signature, sent on the same channel as the data. The transmitter employs a listener antenna and performs signature correlation to discern this notification. Once discerned, the transmitter immediately aborts the transmission. We show that the notification signature can be reliably detected at the listener antenna, even in the presence of a strong self-interference from the transmit antenna. A prototype testbed of 10 USRP/GNU Radios demonstrates the feasibility and effectiveness of CSMA/CN.
Synthesis of substituted indenones and indanones by a Suzuki–Miyaura coupling/acid-promoted cyclisation sequence (2013)

A one-pot Suzuki-Miyaura cross-coupling/acid-catalyzed cyclisation leading to indenones and indanones in modest to good yields is reported.
Progress toward the total synthesis of (-)-cylindrocyclophane A (1998)

The study has received ethical approval from the Ethical Review Committee of the Faculty of Medicine and Allied Sciences, Rajarata University of Sri Lanka. A sensitive data collection technique will be used and ethical issues will be considered throughout the study. Results will be disseminated in scientific peer-reviewed articles.
Axon morphology is modulated by the local environment and impacts the noninvasive investigation of its structure–function relationship (2020)

Axonal conduction velocity, which ensures efficient function of the brain network, is related to axon diameter. Noninvasive, in vivo axon diameter estimates can be made with diffusion magnetic resonance imaging, but the technique requires three-dimensional (3D) validation. Here, high-resolution, 3D synchrotron X-ray nano-holotomography images of white matter samples from the corpus callosum of a monkey brain reveal that blood vessels, cells, and vacuoles affect axonal diameter and trajectory. Within single axons, we find that the variation in diameter and conduction velocity correlates with the mean diameter, contesting the value of precise diameter determination in larger axons. These complex 3D axon morphologies drive previously reported 2D trends in axon diameter and <i>g</i>-ratio. Furthermore, we find that these morphologies bias the estimates of axon diameter with diffusion magnetic resonance imaging and, ultimately, impact the investigation and formulation of the axon structure-function relationship.
Selection of Human Anti-CD22 scFvs from an Acute Lymphocytic Leukemia Related scFv Library with Ribosome Display. (2006)

Abstract Novel in vitro methods for screening of antibody libraries against disease-related antigens have led to the development of powerful protein-based biotherapeutics. Ribosome display represents an emerging and innovative in vitro technology for the rapid isolation and evolution of high-affinity peptides or proteins. Eukaryotic ternary ribosome complexes can be used to display human single chain antibodies (scFvs) in order to isolate specific binding reagents. Here we present the isolation of human scFv against the validated immunotherapeutic target antigen CD22 from a leukaemia patient-derived human scFv library using ribosome display technology. The ribosome-displayed scFv were enriched against the human CD22 conjugated to magnetic beads. Isolated constructs were further affinity-matured and specific target recognition was demonstrated by surface plasmon resonance and validated using in vitro cell assays.
Independent Factors Predicting Postoperative 30-Day Mortality in 101 Infants Following Liver Transplantation (2017)

BACKGROUND Liver transplantation (LT) in infants is a challenging procedure. This study was designed to identify risk factors of 30-day mortality of infant LT recipients. MATERIAL AND METHODS A total of 101 infants treated between May 1997 and March 2015 were assigned to one of two groups, a deceased infant group (n=9) or a live infant group (n=92), according to their mortality status at 30 days after LT. RESULTS Patient characteristics of height, weight, preoperative total bilirubin, creatinine clearance, primary diagnosis, and graft-to-recipient weight ratio (GRWR) were significantly different between the two groups. In a binary logistic regression model for multivariate analysis, independent predictors of postoperative mortality in infants after LT were hospitalization in the intensive care unit before LT, reoperation within 30 days of the first operation, and high GRWR (>4). CONCLUSIONS Postoperative mortality of infants following LT showed significant correlations with recipient preoperative and postoperative conditions and also with graft size mismatch. Regarding GRWR, our data indicated that relatively large grafts given to infant recipients often lead to graft failure or primary nonfunction during the postoperative stage.
Supplementary Figure 2, Video 4 from Enhancement of Human Cancer Cell Motility and Invasiveness by Anaphylatoxin C5a via Aberrantly Expressed C5a Receptor (CD88) (2023)

&lt;p&gt;AVI file - 16396K, Time-lapse video analysis-C5aR(+)C5a(+)&lt;/p&gt;