gebhart/patrepeval huggingface dataset:
Biodistribution study of 99mTc‐labeled LDL in B16‐melanoma‐bearing mice. Visualization of a preferential uptake by the tumor (1993)

Since there is strong evidence of a preferential LDL accumulation in tumor cells, LDL might be of interest for tumor imaging. We have tested the ability of 99mTc-LDL in tumor imaging with B16-melanoma-bearing mice as a model for further applications in human studies. The LDL fixation rate was higher with 99mTc-labeled LDL than with 125I labeled LDL. Since technetium-99m remains trapped in the cells, 99mTc-LDL is a well-adapted radioligand because of information given by this radiotracer on the receptor metabolism. We observed that, at early growth stages, the tumor took up the LDL at a maximal rate, suggesting differences in cholesterol metabolism as a function of tumor growth. Accumulation of label in the tumor area was perfectly observable in tumor-bearing mice on scintigraphic images. Computerized quantification of the regions of interest (as well as biodistribution studies including killing of the animals) showed a 1.81-fold increase in uptake by the tumor as compared to the liver and a 28-fold increase as compared with corresponding normal tissue (muscle of the left leg) at day 8 of tumor growth. These data give strong support to the value of this non-invasive method in visualizing and quantifying the tissue LDL uptake in vivo, including the precise information provided by nuclear scintigraphy on the distribution of the radiolabeled LDL in the different tissues. 99mTc-LDL could be an efficient tool for further diagnostic or therapeutic exploration in cancer patients.
Supplementary Figure 1 from ICAM-1 Has a Critical Role in the Regulation of Metastatic Melanoma Tumor Susceptibility to CTL Lysis by Interfering with PI3K/AKT Pathway (2023)

Supplementary Figure 1 from ICAM-1 Has a Critical Role in the Regulation of Metastatic Melanoma Tumor Susceptibility to CTL Lysis by Interfering with PI3K/AKT Pathway
Convex Optimization for Low-Complexity Spatial Antenna Arrays (2012)

Conventional smart antennas create flexible beam patterns using weights that have both magnitude and phase. This requires expensive hardware in the form of individual receivers and transmitters, high-speed D/A and A/D converters and capable DSP or FPGA processors. This paper looks at the use of low-complexity spatial antenna arrays that can create reasonably complex antenna patterns using a phased array approach. Convex optimization is applied to solve the highly non-linear optimization problem. Square array geometries were studied in depth by applying various penalty functions. We found that convex optimization is a novel and effective way to compute the complex antenna weights, and that this low-complexity approach is an interesting alternative to more expensive smart antennas
Gracilis Urethromyoplasty-An Autologous Urinary Sphincter for Neurologically Impaired Patients With Stress Incontinence (1998)

No AccessJournal of UrologyUrological Survey: Abstracts: Voiding Function and Dysfunction, and Female Urology1 Mar 1998Gracilis Urethromyoplasty-An Autologous Urinary Sphincter for Neurologically Impaired Patients With Stress Incontinence M.B. Chancellor, R.D. Hong, D.A. Rivas, T. Watanabe, J.-A. Crewalk, and I. Bourgeois M.B. ChancellorM.B. Chancellor More articles by this author , R.D. HongR.D. Hong More articles by this author , D.A. RivasD.A. Rivas More articles by this author , T. WatanabeT. Watanabe More articles by this author , J.-A. CrewalkJ.-A. Crewalk More articles by this author , and I. BourgeoisI. Bourgeois More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)63873-4AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "Gracilis Urethromyoplasty-An Autologous Urinary Sphincter for Neurologically Impaired Patients With Stress Incontinence." The Journal of Urology, 159(3), pp. 1114–1115 Department of Urology, Thomas Jefferson University, Philadelphia, Division of Urologic Surgery, University of Pittsburgh, School of Medicine, Pittsburgh, Pennsylvania, and Bakken Research Center, Maastricht, The Netherlands.© 1998 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 159Issue 3March 1998Page: 1114-1115 Advertisement Copyright & Permissions© 1998 by American Urological Association, Inc.MetricsAuthor Information M.B. Chancellor More articles by this author R.D. Hong More articles by this author D.A. Rivas More articles by this author T. Watanabe More articles by this author J.-A. Crewalk More articles by this author I. Bourgeois More articles by this author Expand All Advertisement PDF downloadLoading ...
Highly efficient PD-1-targeted CRISPR-Cas9 for tumor-infiltrating lymphocyte-based adoptive T cell therapy (2022)

Adoptive T cell therapy (ACT) with expanded tumor-infiltrating lymphocytes (TIL) can induce durable responses in cancer patients from multiple histologies, with response rates of up to 50%. Antibodies blocking the engagement of the inhibitory receptor programmed cell death protein 1 (PD-1) have been successful across a variety of cancer diagnoses. We hypothesized that these approaches could be combined by using CRISPR-Cas9 gene editing to knock out PD-1 in TILs from metastatic melanoma and head-and-neck, thyroid, and colorectal cancer. Non-viral, non-plasmid-based PD-1 knockout was carried out immediately prior to the traditional 14-day TIL-based ACT rapid-expansion protocol. A median 87.53% reduction in cell surface PD-1 expression was observed post-expansion and confirmed at the genomic level. No off-target editing was detected, and PD-1 knockout had no effect on final fold expansion. Edited cells exhibited few phenotypic differences and matched control functionality. Pre-clinical-scale results were confirmed at a clinical scale by generating a PD-1-deficient TIL product using the good manufacturing practice facilities, equipment, procedures, and starting material used for standard patient treatment. Our results demonstrate that simple, non-viral, non-plasmid-based CRISPR-Cas9 methods can be feasibly adopted into a TIL-based ACT protocol to produce treatment products deficient in molecules such as PD-1, without any evident negative effects.
Evaluation of Marrow Perfusion in the Femoral Head by Dynamic Magnetic Resonance Imaging Effect of Venous Occlusion in a Dog Model (1992)

Dynamic Gd-DTPA-enhanced fast MRI technique can detect acute changes in bone marrow perfusion due to venous occlusion. This technique may have applications in the early detection of nontraumatic AVN.
A Non-Linear Lumped Model for the Electro-Mechanical Coupling in Capacitive MEMS Microphones (2021)

This work provides an analytical non-linear model for the capacitive transduction in MEMS transducers with perforated counter-electrodes, especially applicable to capacitive MEMS microphones. Starting from an electrostatic description of a perforated unit cell of the transducer, analytical formulations of the variable capacitance and electrostatic forces are derived, accounting for the deflection profile of a clamped circular plate. A lumped implementation into conventional circuit simulations tools is enabled via behavioral modeling based on hardware description languages, such as Verilog-A. Therefore, the analytical model is approximated via Taylor series expansions, allowing for a stable and non-linear behavioral implementation. The resulting model finally enables both a small- and large-signal analysis of capacitive MEMS microphones, precisely accounting for non-linearities in the capacitive transduction. This allows to simulate the harmonic distortion of the microphone's output signal and to account for electrostatic spring-softening in simulations of its bias voltage dependent sensitivity.
Elucidating the Photoredox Nature of Isolated Iron Active Sites on MCM-41 (2017)

Photocatalytic performance is highly dependent on the nature and dispersion of the active sites, playing a crucial role in the optoelectronic and charge-transfer processes. Here, we report stabilized isolated iron on MCM-41 as a highly active catalyst for a photoredox reaction. The unique nature of the single-atom centers exhibit a trichloroethylene conversion per iron site that is almost 5 times higher than that of TiO2. Advanced characterization and theoretical calculations indicate the generation of hydroxyl radicals, through a photoinduced ligand-to-metal charge-transfer mechanism, which act as hole scavengers that lead to the formation of intermediate oxo–iron species (Fe═O). This intermediate species is the key step in promoting the photocatalytic reactions. Understanding the mechanistic photoredox pathway in isolated active site materials is imperative for developing highly efficient nonprecious photocatalysts for environmental or energy applications.
Effects of Adjuvant Sorafenib and Sunitinib on Cardiac Function in Renal Cell Carcinoma Patients without Overt Metastases: Results from ASSURE, ECOG 2805 (2015)

In the adjuvant setting, we prospectively define low incidence of cardiotoxicity with sunitinib and sorafenib. These findings may be related to close CV monitoring, or potentially to fewer CV comorbidities in our nonmetastatic population.
Supplementary Data 7 from SLFN5 Regulates LAT1-Mediated mTOR Activation in Castration-Resistant Prostate Cancer (2023)

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Large perpendicular magnetic anisotropy at Fe/MgO interface (2013)

A large perpendicular magnetic anisotropy (PMA) of 1.4 MJ/m3 was observed from ultrathin Fe/MgO(001) bilayers grown on Cr-buffered MgO(001). The PMA strongly depends on the surface state of Fe prior to the MgO deposition. A large PMA energy density of 1.4 MJ/m3 was achieved for a 0.7 nm thick Fe layer having adsorbate-induced surface reconstruction, which is likely to originate from oxygen atoms floating up from the Cr buffer layer. This large magnitude of PMA satisfies the criterion that is required for thermal stability of magnetization in a few tens nanometer-sized magnetic memory elements.
Spongy Graphene as a Highly Efficient and Recyclable Sorbent for Oils and Organic Solvents (2012)

Abstract In this work, spongy graphene (SG), a shape‐mouldable and nanoporous material with a high specific surface area used as a versatile and recyclable sorbent material, is proposed and studied. SG shows highly efficient absorption of not only petroleum products and fats, but also toxic solvents such as toluene and chloroform (up to 86 times of its own weight), requiring no further pretreatment, which is tens of times higher than that of conventional absorbers. Moreover, SG can be regenerated (&gt;10 times) by heat treatment, yielding the full release of adsorbates (&gt;99%). The present work suggests SG a widespread potential for applications in industry as well as topics regarding environmental protection.
Fairness Enhancement of UAV Systems with Hybrid Active-Passive RIS (2023)

We consider unmanned aerial vehicle (UAV)-enabled wireless systems where downlink communications between a multi-antenna UAV and multiple users are assisted by a hybrid active-passive reconfigurable intelligent surface (RIS). We aim at a fairness design of two typical UAV-enabled networks, namely the static-UAV network where the UAV is deployed at a fixed location to serve all users at the same time, and the mobile-UAV network which employs the time division multiple access protocol. In both networks, our goal is to maximize the minimum rate among users through jointly optimizing the UAV's location/trajectory, transmit beamformer, and RIS coefficients. The resulting problems are highly nonconvex due to a strong coupling between the involved variables. We develop efficient algorithms based on block coordinate ascend and successive convex approximation to effectively solve these problems in an iterative manner. In particular, in the optimization of the mobile-UAV network, closed-form solutions to the transmit beamformer and RIS passive coefficients are derived. Numerical results show that a hybrid RIS equipped with only 4 active elements and a power budget of 0 dBm offers an improvement of 38%-63% in minimum rate, while that achieved by a passive RIS is only about 15%, with the same total number of elements.
Cutting Edge: Recipient MHC Class II Expression Is Required to Achieve Long-Term Survival of Murine Cardiac Allografts After Costimulatory Blockade (2001)

To study the role of the direct and indirect pathways in achieving tolerance, we used genetically altered mouse strains in two ways: 1) MHC class II-deficient mice were used as donors of skin and cardiac grafts to eliminate the direct CD4(+) T cell response, and 2) B6 II(-)4(+) mice, which are MHC class II-deficient mice expressing an MHC class II transgene only on thymic epithelium, were used as recipients of normal grafts. These mice cannot mount an indirect response. Eliminating the indirect pathway actually made it more difficult to achieve prolonged allograft survival when we used costimulatory blockade than when both pathways were available. Costimulatory blockade was ineffective even when CD4(+) T cells from normal animals were transferred into recipients that lacked MHC class II molecules. These results suggest that an active CD4(+) response through the indirect pathway is necessary for costimulatory blockade to be effective in prolonging allograft survival.
Variational approach for segmentation of lung nodules (2011)

Lung nodules from low dose CT (LDCT) scans may be used for early detection of lung cancer. However, these nodules vary in size, shape, texture, location, and may suffer from occlusion within the tissue. This paper presents an approach for segmentation of lung nodules detected by a prior step. First, regions around the detected nodules are segmented; using automatic seed point placement levels sets. The outline of the nodule region is further improved using the curvature characteristics of the segmentation boundary. We illustrate the effectiveness of this method for automatic segmentation of the Juxta-pleural nodules.
Decreased accumulation of [14c]carboplatin in human cisplatin-resistant cells results from reduced energy-dependent uptakeThis article is a US Government work and, as such, is in the public domain in the United States of America. (2000)

We have isolated cisplatin-resistant human liver carcinoma (7404-CP20) cells with reduced accumulation of cisplatin and other drugs (methotrexate, arsenate, and arsenite) to which these cells are cross-resistant. To determine whether the reduction of drug accumulation in cisplatin-resistant cells results from impaired uptake or from active efflux, [14C]carboplatin was used for kinetic analysis of drug uptake and efflux. We demonstrate here that the uptake of [14C]carboplatin in 7404 parental cells is time, temperature, and energy dependent, and that the rate of uptake is reduced in 7404-CP20 cells. Efflux of [14C]carboplatin in cisplatin-resistant cells was comparable to efflux in the parental cisplatin-sensitive cells. There was little effect of temperature (between 37°C and 4°C) on efflux in cisplatin-resistant cells. Immunoblotting with specific antibodies directed to MRP1 and MRP2 (cMOAT) also showed that expression of these two ABC transporter genes was considerably reduced in 7404-CP20 cells and another cisplatin-resistant cell line KB-CP20, in contradistinction to previous studies suggesting that MRP might be responsible for cisplatin efflux. To rule out a generalized defect in uptake of small molecules, fluorescence-activated cell sorter (FACS) analysis of rhodamine 123 uptake showed that there was no difference between cisplatin-sensitive and -resistant cells. The presence of a pleiotropic defect in uptake of [14C]carboplatin, [3H]methotrexate, [73As]arsenate, and [73As]arsenite in cisplatin-resistant cells, in association with reduced expression of related cell surface proteins as demonstrated in our previous work, suggests a novel mechanism for acquisition of resistance to cisplatin associated with reduced activity of many different specific uptake systems. J. Cell. Physiol. 183:108–116, 2000. © 2000 Wiley-Liss, Inc.
State-of-the-art device in high voltage power ICs with lowest on-state resistance (2010)

High performance LDMOS of 600-800V Vbdss has been developed with PB (Pbody)_Extension RESURF scheme for smart power applications. This device design demonstrates the lowest specific on-state resistance against to the latest publications (40% improvement than triple RESURF, 65% improvement than double RESURF in JI LDMOSFET) in 600-800V families and breaks 1-D silicon limit. This technology also surpasses the performance in thin SOI technology, yet it uses the bulk Si material and less manufacturing processing steps.
Integral Action NMPC for Tight Maneuvers of Articulated Vehicles (2023)

We propose an integral action nonlinear model predictive controller (NMPC) for trajectory tracking of an articulated vehicle with an uncertain hitching offset. The controller is intended for complex parking maneuvers including forward and backward movement with tight specifications on the lateral positional tracking error of the trailer. In order to assess performance with uncertain hitching offsets, disturbances, and sensor noise, we conduct extensive hardware-in-the-loop simulations using a dSPACE Scalexio unit. With high-grade sensing, we demonstrate that the closed-loop control system achieves a lateral tracking error of < 3 [cm] in expectation, and an absolute terminal error of < 15 [cm] with high probability p > 0.97. The proposed integral action is shown to be essential in achieving this performance, and the efficacy of the proposed NMPC is evaluated by comparison to alternative MPCs.
Gene Therapy and Imaging of Transgene Expression in Living Subjects (2010)

Broadly defined, gene therapy is the process of delivering genetic materials into a patient or cells from the patient to modify the expression of one or more genes to achieve a therapeutic outcome for the patient. Hence, a transgene delivery procedure may be intended for the following reasons: (1) to initiate the expression of the transgene in specific tissues or the entire body of the patient, (2) to reduce the expression of specific endogenous genes at a target site or the entire body of the patient, and (3) to enhance the expression of specific endogenous genes in target tissues or the entire body of the patient. The success of a gene therapy procedure depends on several factors including (1) specific cell targeting when it is necessary to cause changes in gene expression only in specific cells of the patient's body and avoid modification of gene expression in all other cells. This may be necessary to avoid side effects and deliver sufficient gene expression modifying genetic material to the target cells; (2) efficiency of transgene delivery to achieve sufficient modification of gene expression in target cells; (3) modified gene expression for sufficient time duration; and (4) lack of immune reaction to the product of the therapeutic transgene, when immune reaction will reduce efficacy or potency of the therapeutic procedure. Investigators in the field of gene therapy are working hard to solve problems associated with all of these factors.
Quantitative imaging reveals heterogeneous growth dynamics and treatment-dependent residual tumor distributions in a three-dimensional ovarian cancer model (2010)

Three-dimensional tumor models have emerged as valuable in vitro research tools, though the power of such systems as quantitative reporters of tumor growth and treatment response has not been adequately explored. We introduce an approach combining a 3-D model of disseminated ovarian cancer with high-throughput processing of image data for quantification of growth characteristics and cytotoxic response. We developed custom MATLAB routines to analyze longitudinally acquired dark-field microscopy images containing thousands of 3-D nodules. These data reveal a reproducible bimodal log-normal size distribution. Growth behavior is driven by migration and assembly, causing an exponential decay in spatial density concomitant with increasing mean size. At day 10, cultures are treated with either carboplatin or photodynamic therapy (PDT). We quantify size-dependent cytotoxic response for each treatment on a nodule by nodule basis using automated segmentation combined with ratiometric batch-processing of calcein and ethidium bromide fluorescence intensity data (indicating live and dead cells, respectively). Both treatments reduce viability, though carboplatin leaves micronodules largely structurally intact with a size distribution similar to untreated cultures. In contrast, PDT treatment disrupts micronodular structure, causing punctate regions of toxicity, shifting the distribution toward smaller sizes, and potentially increasing vulnerability to subsequent chemotherapeutic treatment.
A multi-technique, micrometer- to atomic-scale description of a synthetic analogue of chukanovite, Fe2(CO3)(OH)2 (2014)

A synthetic analogue of chukanovite Fe2(CO3)(OH) 2 is formed during experimental work on iron-clay interactions simulating the cooling of containers in radioactive waste repositories. Despite its small size and the mixture with other minerals it is undoubtedly identified by X-Ray Diffraction, Fourier Transform Infrared Spectroscopy, Scanning Electron Microscopy and Transmission Electron Microscopy. For the first time, the structural characterisation of a synthetic chukanovite is carried out thanks to the combination of Automated Diffraction Tomography and Precession Electron Diffraction. Refinement results and comparison with literature data show that the structure of this synthetic chukanovite is consistent with that proposed for natural chukanovite found in Dronino meteorite. © 2014 E. Schweizerbart'sche Verlagsbuchhandlung.
Low-stress silicon nitride platform for broadband mid-infrared microphotonics (2014)

We experimentally demonstrate a sophisticated mid-IR microphotonics platform adopting engineered Si-rich and low-stress silicon nitride (SiNx) thin films where an extensive infrared transparency up to λ = 8.5 µm is achieved. Furthermore, because of the designed low-stress property, the SiNx deposition is able to reach a thickness > 2 µm that significantly reduces mid-IR waveguide loss to less than 0.2 dB/cm. We show directional couplers functioning over a broad infrared spectrum, thus enabling monolithic mid-IR multiplexing schemes for integrated linear and nonlinear photonics leading to sophisticated label-free sensing technologies.
Eucalyptus oleosa Essential Oils: Chemical Composition and Antimicrobial and Antioxidant Activities of the Oils from Different Plant Parts (Stems, Leaves, Flowers and Fruits) (2011)

Essential oils obtained by hydrodistillation from the different parts (stems, adult leaves, immature flowers and fruits) of Eucalyptus oleosa were screened for their antioxidant and antimicrobial properties and their chemical composition. According to GC-FID and GC-MS, the principal compound of the stem, immature flowers and the fruit oils was 1,8-cineole, representing 31.5%, 47.0% and 29.1%, respectively. Spathulenol (16.1%) and γ-eudesmol (15.0%) were the two principal compounds of adult leaves oil. In the DPPH (1,1-diphenyl-2-picrylhydrazyl) assay, the oils of the four parts showed moderate antioxidant activity. In the ABTS (2,2'-azinobis-3-ethylbenzothiazoline-6-sulphonate) assay, the most active part was the adult leaves, with a IC(50) value 13.0 ± 0.6 mg/L, followed by stems (IC(50) = 43.5 ± 1.4 mg/L). The essential oils showed a better antibacterial activity against Gram-positive and Gram-negative bacteria, and a significant antifungal activity also was observed against yeast-like fungi. A strong correlations between oxygenated monoterpenes and antimicrobial activity (especially 1,8-cineole) were noted (R2 = 0.99, 0.97 and 0.79 for B. subtilis, P. aeruginosa and C. albicans, respectively).