1669 NERVE GROWTH FACTOR LEVELS IN URINE CORRELATE WITH EXPRESSION IN UROTHELIUM BUT NOT SUBUROTHELIUM IN THE BLADDER TISSUES OF OVERACTIVE BLADDER SYNDROME (2010)

You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Female Urology II1 Apr 20101669 NERVE GROWTH FACTOR LEVELS IN URINE CORRELATE WITH EXPRESSION IN UROTHELIUM BUT NOT SUBUROTHELIUM IN THE BLADDER TISSUES OF OVERACTIVE BLADDER SYNDROME Hsin-Tzu Liu, Yue-Shiung Wang, Michael Chancellor, and Hann-Chorng Kuo Hsin-Tzu LiuHsin-Tzu Liu Hualien, Taiwan More articles by this author , Yue-Shiung WangYue-Shiung Wang Hualien, Taiwan, Taiwan More articles by this author , Michael ChancellorMichael Chancellor Royal Oak, MI More articles by this author , and Hann-Chorng KuoHann-Chorng Kuo Hualien, Taiwan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.1493AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Urinary nerve growth factor (NGF) has gained great interest in detecting detrusor overactivity (DO) in patients with overactive bladder syndrome (OAB). However, the source of increased urinary NGF in OAB patients has not been fully investigated. In this study we investigated the relationship of urinary NGF levels and NGF expression in the urothelium and suburothelium in patients with OAB. METHODS Bladder biopsies not involving muscle layer were obtained from patients with OAB and controls. Urine samples at full bladder capacity were also collected for urinary NGF measurement prior to the biopsy. Urinary NGF levels were measured by the Emax ImmunoAssay System ELISA Kit. Expression of NGF in urothelium and suburothelium were immunohistochemically stained using anti-human beta-NGF antibody and stained sections were captured by digital image system. The digital images were converted to grey-level and density of NGF expression was quantified with the Image J software. (Fig) Three tissue sections per patient were quantified and the mean values were used in subsequent statistical analysis. Correlation analysis between NGF in urothelium and suburothelium and urinary NGF were performed. Six bladder and urine samples obtained from patients without lower urinary tract symptoms served as controls. RESULTS The NGF expressions in urothelial (118+/-20) and suburothelial (360+/-223) correlated well in bladder tissue of OAB (p=0.023). However, NGF expressions in the control bladders, both in urothelium (139+/-19) and suburothelium (491+/-189), were also noted. Urinary NGF levels were significantly greater in OAB (0.60/-1.03 pg/ml) compared to that in controls (0 pg/ml). There were statistically significant correlation between urinary NGF levels and NGF expression in urothelium (p=0.05), but not with NGF expression in suburothelium (p=0.694) in OAB patients. CONCLUSIONS Urinary NGF levels increased in patients with OAB and correlated well with NGF expression in the urothelium but not suburothelium, suggesting the source of NGF in urine was from urothelium in OAB. Although there was abundant NGF expression in urothelium and suburothelium of the controls, the urinary NGF level was absent. This result implies that the urothelial barrier might have defect in OAB. © 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e644-e645 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.MetricsAuthor Information Hsin-Tzu Liu Hualien, Taiwan More articles by this author Yue-Shiung Wang Hualien, Taiwan, Taiwan More articles by this author Michael Chancellor Royal Oak, MI More articles by this author Hann-Chorng Kuo Hualien, Taiwan More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...