gebhart/patrepeval huggingface dataset:
Generation of Oligomeric Insulin Receptor Forms by Intramolecular Sulfhydryl-Disulfide Exchange. Involvement of Masked Sulfhydryl Groups (1987)

Insulin receptors from rat liver membranes were labelled with a 125I-labelled photoreactive insulin analogue or by iodination using lactoperoxidase and analysed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Under nonreducing conditions different receptor forms with Mr 400,000 (alpha 2 beta 2), 360,000 (alpha 2 beta beta'), 330,000 (alpha 2 beta' beta'), 320,000 (alpha 2 beta), 280,000 (alpha 2 beta'), 240,000 (alpha 2), 210,000 (alpha beta), 165,000 (alpha beta') and 115,000 (alpha) were detected. The subunit composition of these receptor forms was determined by two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis in the absence and presence of dithioerythritol. During denaturation in sodium dodecyl sulfate in the absence of reductants, the Mr 400,000 receptor form (alpha 2 beta 2) was converted into the Mr 320,000 (alpha 2 beta) and Mr 240,000 (alpha 2) receptor form. This conversion was prevented either by N-ethylmaleimide, oxidants, or low pH. In contrast, alkylation of the receptor with N-ethylmaleimide under non-denaturing conditions did not prevent the appearance of intermediate-sized receptor forms. Furthermore, the inhibition of receptor cleavage by N-ethylmaleimide during denaturation was also observed when the amount of free sulfhydryl groups was reconstituted by the addition of an unlabelled and non-alkylated receptor sample to the alkylated and photoaffinity-labelled receptor. These results suggest, that the generation of different oligomeric receptor forms detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis is due at least in part to the cleavage of one or both beta-subunits from the insulin receptor.(ABSTRACT TRUNCATED AT 250 WORDS)
Abstract B10: ER stress protein GRP78 as a therapeutic target combined with antiangiogenic therapy in renal cell carcinoma (2013)

Abstract Background: Antiangiogenic therapy deprives oxygen and nutrition from the tumor. These stress conditions cause unfolded or misfolded proteins in tumor cells. Glucose regulated protein 78 (GRP78) binds to unfolded proteins and dissociates from the membrane-bound ER stress sensors. Dissociation of GRP78 from these stress sensors allows their subsequent activation which suppresses global mRNA translation to protect cells from excessive unfolded proteins. We investigated a potential role of GRP78 as a combined therapeutic target in renal cell carcinoma treated with antiangiogenic therapy. Methods: Renal cell carcinoma cells (Caki-1, Caki-2, UMRC-3 and UMRC-6) were used to investigate the effect of GRP78 knockdown, which was performed by small interfering RNA. Caki-1 xenografts were developed and treated with sunitinib 40mg/kg/day to evaluate in vivo expression of GRP78 during antiangiogenic therapy. Caki-1 cells stably overexpressing GRP78 were developed to investigate the role of GRP78 in cancer cell. Hypoxic stress was induced by 1% hypoxia chamber and hypoglycaemic stress was induced by glucose-free media. Downstream signalling pathways of GRP78 were evaluated by Western blots. Results: In vitro hypoxia and/or glucose deprivation induced GRP78 upregulation in Caki-1 cells. GRP78 was also induced in Caki-1 xenografts treated by sunitinib. Overexpression of GRP78 increased tumor proliferation in hypoxic and/or hypoglycemic stresses by activating PERK/eIF2α pathway and protected tumor cells from stress-induced apoptosis. Knockdown of GRP78 using small interference RNA inhibited cancer cell survival and induced apoptosis in renal cell carcinoma cells in vitro. GRP78 knockdown also sensitized renal cell carcinoma cells to ER stress-induced apoptosis and hypoxic and hypoplycemic stress-induces apoptosis. Conclusions: Antiangiogenic therapy induces ER stress by depriving oxygen and glucose from renal cell carcinoma. ER protein GRP78 has a critical role in protecting renal cell carcinoma cells from hypoxic and hypoglycemic stress induced by antiangiogenic therapy. Knockdown of GRP78 sensitizes RCC cells to apoptotic cell death from anti-angiogenic stresses. Our results suggest that GRP78 is a novel therapeutic target in renal cell carcinoma management. Citation Format: Kyung Seok Han, Na Li, Estelle Li, Fazli Ladan, Gleave Martin, Alan So. ER stress protein GRP78 as a therapeutic target combined with antiangiogenic therapy in renal cell carcinoma. [abstract]. In: Proceedings of the AACR Precision Medicine Series: Synthetic Lethal Approaches to Cancer Vulnerabilities; May 17-20, 2013; Bellevue, WA. Philadelphia (PA): AACR; Mol Cancer Ther 2013;12(5 Suppl):Abstract nr B10.
The lens capsule and rubeosis iridis: an angiographic study. (1984)

In 15 pigmented rabbits a pars plana lensectomy, vitrectomy and giant retinal tear with bullous retinal detachment were produced with maintenance of an intact anterior lens capsule. The incidence of subsequent rubeosis iridis, as detected angiographically, was compared with that in a control group of animals in whom the anterior lens capsule was not preserved but whose management was identical in all other respects. The rubeosis in the rabbits was angiographically identical to that seen in humans following vitrectomy, with immediate formation of a mossy hyperfluorescent surface membrane that leaked fluorescein extensively into the anterior chamber in the late phase of the angiography. It was possible to distinguish true neovascularization from breakdown of the blood-ocular barrier by the time and extent of the fluorescein leakage. Preservation of an intact anterior lens capsule reduced the incidence of unequivocal (florid) rubeosis from 73% to 33% (p less than 0.05).
Wireless sensor networks for ambient intelligence (2013)

La presente tesis se enmarca en el campo de la asistencia a la vida en el hogar (del ingles Ambient Assisted Living, AAL), una rama de la inteligencia ambiental que trata de mejorar la vida en el hogar mediante tecnicas de inteligencia artificial. El objetivo global de la tesis es lograr avances significativos en eficiencia y prestaciones en las tecnologias aplicadas para la sensorizacion en este campo. Estos avances tecnologicos afectan a varios aspectos y niveles dentro de la capa de captacion sensorial de estos sistemas, resultando en una serie de objetivos especificos: (1) Reduccion del numero de transmisiones mediante pre-procesamiento de la senal sensorial y fusion de datos. (2) Deteccion de ocupacion en cama o sillon: mejora de las prestaciones y la fiabilidad. (3) Refuerzo de la informacion contextual mediante el uso de sensores portables. (4) Deteccion de actividad: reconocimiento de patrones y clasificacion de senal. (5) Mejora de las prestaciones mediante integracion microelectronica. El trabajo llevado a cabo durante el periodo de investigacion ha resultado en diversas contribuciones sobre los objetivos de la tesis.
Con respecto al objetivo (1), se ha disenado un nuevo middleware que incorpora una maquina de estados que introduce un nuevo nivel de abstraccion, generando informacion mas elaborada. De esta forma, se ha eliminado un gran numero de transmisiones asociadas a eventos irrelevantes, generados durante transitorios en el contexto ambiental. Se ha propuesto, a su vez, un mecanismo de asentimiento especifico para AAL.
Para alcanzar el objetivo (2), se han mejorado las prestaciones del sistema AAL mediante un nuevo sensor de ocupacion. Este nuevo diseno ha sido posible gracias a la caracterizacion de un nuevo modelo para el transductor basado en EMFi (ElectroMechanical Film). Por otro lado, se ha propuesto un metodo de umbralizacion adaptativo para los sensores de ocupacion basados en transductores de peso.
Se ha propuesto un metodo que emplea un conjunto mixto de sensores (fijos y portables), en cumplimiento del objetivo (3), incorporando un sensor opcional basado en acelerometros. Ademas, se ha implementado y testado un prototipo del nodo sensor portable.
El cumplimiento del objetivo (4) ha sido aproximado mediante el diseno de un metodo de clasificacion de senales de sensores PIR (Passive InfraRed), atendiendo a la velocidad y distancia entre el sujeto y un sensor multiple. Este metodo permite obtener dispositivos compactos que llevan a cabo computacion local y son capaces de transmitir informacion contextual de nivel de abstraccion medio de una forma eficiente.
Finalmente, atendiendo al objetivo (5), se ha desarrollado un filtro CMOS (Complementary Metal-Oxide-Semiconductor ) pasa-banda de 4o orden con bajo consumo para sensores PIR. Los resultados de simulacion obtenidos a nivel de esquematico han presentado muy bajo consumo y un nivel de ruido integrado aceptable para la aplicacion senalada.
SU-F-BRCD-09: Total Variation (TV) Based Fast Convergent Iterative CBCT Reconstruction with GPU Acceleration (2012)

We have developed a fast convergence iterative algorithm for CBCT image reconstruction. The developed algorithm yielded images with better spatial resolution and higher SNR than those produced by a commercial FDK tool. In addition, from the few-view study, the iterative algorithm has shown great potential for significantly reducing imaging dose. We expect that the developed reconstruction approach will facilitate applications including IGART and patient daily CBCT-based treatment localization.
CD11c- and CD11b-expressing mouse leukocytes transport single Toxoplasma gondii tachyzoites to the brain (2005)

The protozoan parasite Toxoplasma gondii enters hosts through the intestinal mucosa and colonizes distant tissues such as the brain, where its progeny persists for a lifetime. We investigated the role of CD11c- and CD11b-expressing leukocytes in T. gondii transport during the early step of parasitism from the mouse small intestine and during subsequent parasite localization in the brain. Following intragastric inoculation of cyst-containing parasites in mice, CD11c+ dendritic cells from the intestinal lamina propria, the Peyer patches, and the mesenteric lymph nodes were parasitized while in the blood, parasites were associated with the CD11c- CD11b+ monocytes. Using adoptive transfer experiments, we demonstrated that these parasitized cells triggered a parasitic process in the brain of naive recipient mice. Ex vivo analysis of parasitized leukocytes showed that single tachyzoites remained at the cell periphery, often surrounded by the host cell plasma membrane, but did not divide. Using either a dye that labels circulating leukocytes or an antibody known to prevent CD11b+ circulating leukocytes from leaving the microvascular bed lumen, and chimeric mice in which the hematopoietic cells expressed the green fluorescent protein, we established that T. gondii zoites hijacked CD11b+ leukocytes to reach the brain extravascular space.
Enhanced vision system for laparoscopic surgery (2013)

Laparoscopic surgery offers benefits to the patients but poses new challenges to the surgeons, including a limited field of view. In this paper, we present an innovative vision system that can be combined with a traditional laparoscope, and provides the surgeon with a global view of the abdominal cavity, bringing him or her closer to open surgery conditions. We present our first experiments performed on a testbench mimicking a laparoscopic setup: they demonstrate an important time gain in performing a complex task consisting bringing a thread into the field of view of the laparoscope.
Vertically coupled a-Si:H multimode interference waveguides for multi-layer silicon photonics platform (2019)

We successfully demonstrated low temperature fabrication process of vertical MMI a:Si-H waveguides for multi-layer photonic integrated circuit. Measured MMI loss of 1.97dB/MMI and vertical light coupling of TE polarization at 1550nm wavelength have been achieved.
Digital beam-steering of parametric array sound beams using piezoelectric micromachined ultrasonic transducers (2011)

In a previous study [H. Lee etal., J. Acoust. Soc. Am. 125, 1879–1893 (2009)], a parametric array (PA) transducer in air using micromachined ultrasonic transducers (MUT) was examined as an ultrasonic ranging sensor. It was shown that the piezoelectric micromachined ultrasonic transducer (pMUT) may be effective for PA ultrasonic ranging sensors. Since the pMUT are usually used in their array, PA source using pMUT can steer its directional sound beam by manipulating phases of radiator in the array. The beam steering capability of the PA source can provide very useful function for parametric loudspeakers as well as directional ultrasonic range sensors. In this work, 16-channel pMUT array for digital beam-steering of parametric array was designed and fabricated. Each channel of the pMUT array consisted of 9 PZT unimorph elements. The two resonance frequency-type pMUT units are designed for generating two primary beams. Beam-steering of each primary beam was controlled separately by applying complex weighting to each channel. An acoustic experiment verified the PA beam-steering of the pMUT. The result shows that the proposed pMUT is suitable for digital beam-steering of parametric array and that it is also useful for the parametric loudspeaker and the PA ultrasonic range sensor.
Anti-windup robust controller considering motor dynamics for speed servo system (2013)

A robust servo system is important for improving the performance of motion control systems in several industry applications. In general, a speed servo system has a controller with an integrator, such as a PI controller. When its output variable is saturated by a current and/or voltage limiter, a wind-up phenomenon and an unstable response often occur. We have already proposed an anti-windup algorithm considering voltage saturation for speed servo system, that regulates the current response smoothly and stably. Moreover, we considered the saturation of current and speed for position servo system, and the anti-windup algorithm regulated the speed response stably. However, the speed response has the overshoot, which is caused by current saturation and the speed controller not keeping the response performance. This paper proposes an anti-windup algorithm considering the motor dynamics and current saturation for speed servo system of SPM synchronous motor. The experimental and numerical simulation results confirm that the speed servo system having the proposed algorithm regulates the motor speed smoothly and stably.
Prevalence of sensitization to Tetranychus urticae in greenhouse workers (2000)

In this study, the prevalence of TU sensitization was 25%. There were significant associations with TU allergy and atopy and the time of exposure to TU.
Fundamental and specific steps in Shack-Hartmann wavefront sensor design (2006)

Shack-Hartmann sensors are widely used to measure wavefront aberrations. We present the fundamental and specific engineering steps in the design of Shack-Hartmann wavefront sensors. Typical performance requirements such as sensor dynamic range, sensitivity and accuracy are defined and discussed. We investigate the trade-offs between these performance metrics and the factors affecting the trade-offs. A first order approach for selecting the optimal parameters of the sensor central piece, the lenslet array, is presented. We also propose a quick tolerance analysis method that can predict the wavefront measurement error due to misalignments, using only the ray-tracing software.
Supplementary Data from The Association of PI3 Kinase Signaling and Chemoresistance in Advanced Ovarian Cancer (2023)

&lt;p&gt;PDF file, 80KB, Experiments detailing the validation of immunomagnetic separation and ELISA.&lt;/p&gt;
Wireless personal area networks in telemedical environment (2002)

Presents a new design of a wireless personal area network with physiological sensors for medical applications in a telemedical environment. Intelligent wireless sensors perform data acquisition and limited processing. Individual sensors monitor specific physiological signals (such as EEG, EGG, GSR, etc.) and communicate with each other and the personal server. The personal server integrates information from different sensors and communicates with the rest of the telemedical system as a standard mobile unit. The authors present their prototype implementation of the wireless intelligent sensor (WISE) based on a very low power consumption microcontroller and a DSP-based personal server. In future the authors expect all components of WISE to be integrated in a single chip for use in a variety of new medical applications and sophisticated human computer interfaces.
Synthesis of an α-phosphono-α,α-difluoroacetamide analogue of the diphosphoinositol pentakisphosphate 5-InsP7 (2019)

Diphosphoinositol phosphates (PP-InsPs) are an evolutionarily ancient group of signalling molecules that are essential to cellular and organismal homeostasis. As the detailed mechanisms of PP-InsP signalling begin to emerge, synthetic analogues of PP-InsPs containing stabilised mimics of the labile diphosphate group can provide valuable investigational tools. We synthesised 5-PCF<sub>2</sub>Am-InsP<sub>5</sub> (<b>1</b>), a novel fluorinated phosphonate analogue of 5-PP-InsP<sub>5</sub>, and obtained an X-ray crystal structure of <b>1</b> in complex with diphosphoinositol pentakisphosphate kinase 2 (PPIP5K2). 5-PCF<sub>2</sub>Am-InsP<sub>5</sub> binds to the kinase domain of PPIP5K2 in a similar orientation to that of the natural substrate 5-PP-InsP<sub>5</sub> and the PCF<sub>2</sub>Am structure can mimic many aspects of the diphosphate group in 5-PP-InsP<sub>5</sub>. We propose that <b>1</b>, the structural and electronic properties of which are in some ways complementary to those of existing phosphonoacetate and methylenebisphosphonate analogues of 5-PP-InsP<sub>5</sub>, may be a useful addition to the expanding array of chemical tools for the investigation of signalling by PP-InsPs. The PCF<sub>2</sub>Am group may also deserve attention for wider application as a diphosphate mimic.
21103 Quantitative Evaluation of Mechanical Property for Copper Surface Reacted with Slurry Chemicals (2009)

The effect of Young's modulus of Cu surface layer on polishing properties was evaluated using AFM force-curve. Young's modulus of Cu surface layer was measured by force-indentaion curve. Young's modulus of the reaction layer of Cu-quinaldic acid increased with increasing the concentration of quinaldic acid. Higher friction force was required to start polishing at higher concentration of quinaldic acid. There was a clear linear relationship between Young's modulus of Cu surface layer in Cu-quinaldic acid and friction force that is required to start polishing. However, we could not identify linear relationship between Young's modulus and polishing rate of the reaction layer of Cu-glycine. This is due to the property of the surface layer, Cu-quinaldic shows the hydrophobic and stable, however soluble layer in Cu-glycine.
A Truncated Erythropoietin Receptor That Fails to Prevent Programmed Cell Death of Erythroid Cells (1992)

A form of the human erythropoietin receptor (EPOR) was identified in which the cytoplasmic region is truncated by alternative splicing. The truncated form of the receptor (EPOR-T) is the most prevalent form of EPOR in early-stage erythroid progenitor cells, but the full-length EPOR (EPOR-F) becomes the most prevalent form in late-stage progenitors. EPOR-T can transduce a mitogenic signal. However, cells transfected with EPOR-T are more prone to programmed cell death than those expressing EPOR-F. EPOR-F may transduce a signal to prevent programmed cell death that is independent of the mitogenic signal, and alternative splicing of the EPOR gene may have an important role in erythropoiesis.
The medial condylar wall is a reliable landmark to kinematically align the femoral component in medial UKA: an in‐silico study (2021)

The native orientation of the medial condylar wall seems to be a reliable anatomical landmark for aligning the femoral component in medial KA UKA in both the axial plane and frontal planes. Other assessed landmarks were shown to not be reliable. Clinical and radiographic assessments of the reliability of using the MWMC to set the frontal and axial orientation of the femoral component when performing a medial KA UKA are needed.
Dual-Modality PET–SPECT Image-Guided Pretargeting Delivery in HER2(+) Breast Cancer Models (2021)

Pretargeted drug delivery has been explored for decades as a promising approach in cancer therapy. An image-guided pretargeting strategy significantly enhances the intrinsic advantages of this approach since imaging the pretargeting step can be used for diagnostic purposes, while imaging of the drug delivery step can be utilized to evaluate drug distribution and assess therapeutic response. A trastuzumab (Tz)-based HER2 pretargeting component (Tz-TCO-[<sup>89</sup>Zr-DFO]) was developed by conjugating with <i>trans</i>-cyclooctene (TCO) bioorthogonal click chemistry functional groups and deferoxamine (DFO) to enable radiolabeling with a <sup>89</sup>Zr PET tracer. The drug delivery component (HSA-DM1-Tt-[<sup>99m</sup>Tc-HyNic]) was developed by conjugating human serum albumin (HSA) with mertansine (DM1), tetrazine (Tt) functional groups, and a HyNic chelator and radiolabeling with <sup>99m</sup>Tc. For <i>ex vivo</i> biodistribution studies, pretargeting and delivery components (without drug) were administered subsequently to mice bearing human HER2(+) breast cancer xenografts, and a high tumor uptake of Tz-TCO-[<sup>89</sup>Zr-DFO] (26.4% ID/g) and HSA-Tt-[<sup>99m</sup>Tc-HyNic] (4.6% ID/g) was detected at 24 h postinjection. <i>In vivo</i> treatment studies were performed in the same HER2(+) breast cancer model using PET-SPECT image guidance. The increased tumor uptake of the pretargeting and drug delivery components was detected by PET-CT and SPECT-CT, respectively. The study showed a significant 92% reduction of the relative tumor volume in treated mice (RTV = 0.08 in 26 days), compared to the untreated control mice (RTV = 1.78 in 11 days) and to mice treated with only HSA-DM1-Tt-[<sup>99m</sup>Tc-HyNic] (RTV = 1.88 in 16 days). Multimodality PET-SPECT image-guided and pretargeted drug delivery can be utilized to maximize efficacy, predict therapeutic response, and minimize systemic toxicity.
Android Application Development (2009)

This practical book provides the concepts and code you need to develop software with Android, the open-source platform for cell phones and mobile devices that's generating enthusiasm across the industry. Based on the Linux operating system and developed by Google and the Open Handset Alliance, has the potential to unite a fragmented mobile market. Android Application Development introduces this programming environment, and offers you a complete working example that demonstrates architectural features and APIs. With this book, you will: get a complete introduction to the programming environment, architecture, and tools; build a modular application, beginning with a core module that serves to launch modules added in subsequent chapters; learn the concepts and architecture of a specific feature set, including views, maps, location-based services, persistent data storage, 2D and 3D graphics, media services, telephony services, and messaging; use ready-to-run example code that implements each feature; and, delve into advanced topics, such as security, custom views, performance analysis, and internationalization. The book is a natural complement to the existing documentation provided by Google. Whether you want to develop a commercial application for mobile devices, or just want to create a mobile mashup for personal use, Android Application Development demonstrates how you can design, build, and test applications for the new mobile market.
Understanding Language and Literacy Development is a Matter of Time (2008)

Frontiers Events is a rapidly growing calendar management system dedicated to the scheduling of academic events. This includes announcements and invitations, participant listings and search functionality, abstract handling and publication, related events and post-event exchanges. Whether an organizer or participant, make your event a Frontiers Event!