gebhart/patrepeval huggingface dataset:
TAT RESULTS IN A LONGITUDINAL STUDY OF BEREAVED COLLEGE STUDENTS (1998)

The authors analyzed projective data obtained from 141 college students who wrote stories on three separate occasions to selected cards from the Thematic Apperception Test (TAT). The students included 46 members of support groups for bereaved college students, 34 members of a bereavement control group, and 61 nonbereaved students. The study used a repeated-measures pretest-posttest control group design to gather longitudinal data about the trajectory of bereavement with and without support group intervention. Coders, who reached consistently high interrater reliability, looked for themes of death, grief, coping, and affiliation in the stories. Multivariate analysis of variance (MANOVA) and repeated-measures MANOVA tests were applied to analyze coding results. Overall MANOVA results indicated significant group differences in the responses to the TAT cards. Repeated-measures MANOVA found group differences in use of themes of death and grief and found Group x Time differences in maintaining a sense of self-efficacy while in a crisis. A majority of the stories contained affiliation imagery but without any group differences in the use of such imagery.
Sonochemical Preparation and Characterization of Europium Oxide Doped In and Coated On ZrO2 and Yttrium-Stabilized Zirconium (YSZ) (2000)

Ultrasound radiation was used to prepare Eu2O3 dd in zirconia and yttrium-stabilized zirconium (YSZ) nanoparticles. Europium oxide was also coated on the surface of submicron spherical zirconia and YSZ, which were fabricated by wet chemical methods. The coating process also used ultrasonic waves. Time decay measurements of the doped and coated materials were conducted using a pulsed laser source. Lifetimes <1.1 ms radiative lifetime of the Eu3+ ion were detected for the doped and coated as-prepared materials. When the doped and coated samples were annealed at 700 °C, longer lifetimes were measured. The shorter lifetimes were attributed to concentration quenching.
ULTRA-SENSITIVE LOCALIZATION AND NEUTRALIZATION OF LOW AND HIGH IMPEDANCE EARTH FAULTS IN DISTRIBUTION NETWORKS (2021)

Three benchmarks are crucial when you look at earth fault protection for power lines: sensitivity (protection shall detect all faults, including high impedance faults); speed (protection needs to operate fast enough to prevent damages and ignition); selectivity (protection should affect the least part of the grid).
Moderate and severe periodontitis are independent risk factors associated with low cardiorespiratory fitness in sedentary non‐smoking men aged between 45 and 65 years (2013)

This study demonstrated that moderate and severe periodontitis were independently associated with low levels of CRF in sedentary men aged between 45 and 65 years.
Coherent flow imaging: A power Doppler imaging technique based on backscatter spatial coherence (2013)

Power Doppler imaging is a commonly used method for the detection of flow. The direction of flow is lost with power Doppler, but it has greater sensitivity to flow compared to velocity estimation techniques. Power Doppler imaging can be degraded if thermal noise is large or if slowly moving reverberation clutter passes through the wall filter.We have developed a power Doppler imaging technique, based on the same principles as power Doppler, but uses the coherence of backscattered blood signal rather than the complex backscattered signal. This technique, called SLSC power Doppler, is less sensitive to clutter-based signals and thermal noise. We show in simulations, phantom, and in vivo experiments that the detected flow demonstrates a smoother, fuller profile and is more robust to the presence of thermal noise. The technique can also utilize fewer ensemble samples to obtain satisfactory detection of flow.
PAK1, a gene that can regulate p53 activity in yeast. (1995)

The ability of p53 protein to activate transcription is central to its tumor-suppressor function. We describe a genetic selection in Saccharomyces cerevisiae which was used to isolate a mutant strain defective in p53-mediated transcriptional activation. The defect was partially corrected by overexpression of a yeast gene named PAK1 (p53 activating kinase), which localizes to the left arm of chromosome IX. PAK1 is predicted to encode an 810-aa protein with regions of strong similarity to previously described Ser/Thr-specific protein kinases. PAK1 sequences upstream of the coding region are characteristic of those regulating genes involved in cell cycle control. Expression of PAK1 was associated with an increased specific activity of p53 in DNA-binding assays accompanied by a corresponding increase in transactivation. Thus, PAK1 is the prototype for a class of genes that can regulate the activity of p53 in vivo, and the system described here should be useful in identifying other genes in this class.
Towards Selective and Sensitive Detection of Carbon Monoxide with CuO/ZnO Heterojunction NanocompositePrepared by an Organometallic Approach (2022)

International audience
Reaction of cobalt porphycene with hydride reagents: spectroscopic detection of Co–H porphycene species and formation of Co–SnR3 porphycene species (2012)

The reaction of tetrapropylporphycenatocobalt(III) with tributyltin hydride generates a cobalt(III)–hydride porphycene detectable by UV-vis spectroscopy under diluted conditions, whereas it is impossible to characterize hydride species of cobalt porphyrins. One of the reasons for the stability of the porphycene hydride species is that the porphycene ring has a lower LUMO energy level due to the decrease in the symmetry of the ligand character. However, the hydride species in a highly concentrated solution of the complex is easily converted into the cobalt(II) species via dimerization or reaction of the hydride with excess tributyltin hydride through hemolysis of the Sn–H/Co–H bonds. When the Co(III) porphycene is reacted with LiBHEt 3 , the final product is the cobalt(III)–ethyl complex formed by β-rearrangement during the reaction of the hydride species and diethylborane in a solvent cage. In the reaction of tetrakistrifluoromethylporphycenatocobalt(III) with tributyltin hydride, the dominant reaction pathway includes one-electron reduction of the porphycene ring together with radical coupling of the tin reagent rather than the net hydride transfer. This finding suggests that the delicate control of the LUMO energy level influences the stability of the hydride species. The tetrapropylporphycenatocobalt(III) complex with tributyltin or triphenyltin hydride in the presence of AIBN produces the corresponding Co(III)– trialkyltin complex. This complex was characterized by 1 H NMR spectroscopy.
Left atrial appendage occlusion for stroke prevention in patients with atrial fibrillation: a systematic review and network meta-analysis of randomized controlled trials (2017)

LAA occlusion appears to preserve the benefits of OAC therapy for stroke prevention in patients with AF, but the current evidence is of low quality.
Remodeling of Centrosomes in Intraspecies and Interspecies Nuclear Transfer Porcine Embryos (2007)

Remodeling of donor cell centrosomes and the centrosome-associated cytoskeleton is crucially important for nuclear cloning as centrosomes are the main microtubule organizing centers that play a significant role in cell division and embryo development. Centrosome dysfunctions have been implicated in various diseases including cancer and metabolic disorders and may also play a role in developmental abnormalities that are frequently seen in cloned animals. In the present studies we investigated microtubule organization and the reorganization and fate of the integral centrosome protein g-tubulin and the centrosome-associated protein centrin in intraspecies (pig oocytes; pig fetal fibroblast cells) and interspecies (pig oocytes; mouse fibroblast cells) reconstructed embryos by using antibodies to g-tubulin or GFP-centrin transfected mouse fibroblasts as donor cells. Microtubules were stained with antibodies to a-tubulin. In-vitro-fertilized oocytes and nuclear transfer (NT) reconstructed oocytes were sequentially analyzed at different developmental stages. Epi-fluorescence results revealed mitotic spindle abnormalities in NT embryos during the first cell cycle (39.4%, 13/33) which were significantly higher than those in IVF embryos (17.0%, 7/41). The abnormalities in IVF embryos are due to polyspermy while the abnormalities in NT embryos are due to donor cell centrosome dysfunctions. In the NT embryos with abnormal microtubule and centrosome organization, g-tubulin staining revealed multipolar centrosome foci while DAPI staining showed misalignment of chromosomes. In intraspecies and interspecies embryos the GFP-centrin signal was detected until 3 hrs after fusion. GFP-centrin was not detected at 8 hrs after NT which is consistent with previous results using anti-centrin antibody staining in intraspecies NT porcine embryos. These data indicate that (1) abnormalities in microtubule and centrosome organization are associated with nuclear cloning at a higher rate than observed in IVF embryos; (2) centrosome and cytoskeletal abnormalities in IVF embryos are due to polyspermy while centrosome and cytoskeletal abnormalities in NT embryos are due to donor cell centrosome dysfunctions; and (3) GFP-centrin of the donor cell centrosome provides a reliable marker to follow its fate in intraspecies reconstructed embryos.
Exosome as a novel nanocarriers for therapeutic delivery (2020)

現在、核酸医薬品や抗体医薬品などの分子標的医薬品の開発が盛んに行われており、その治療効果が期待されている。実際、マウスを用いた治療効果の測定では一定の評価が得られているが、それら評価は腫瘍局所に投与したりして評価している場合もあり、局所投与に限定されるのが現状である。しかし、ヒトの治療を想定したときに、局所投与可能な場合はほとんどなく、開発した医薬品を患部局所に効率的に送達するデリバリーシステムの開発が必要である。エクソソームは細胞が分泌するナノスケールの小胞であり、生体における天然の分子デリバリーシステムを担っている。このエクソソームの特徴に注目した新たなデリバリーシステムの開発が世界中で行われており、治療応用を示した実例も報告されている。しかし、技術的な問題点なども多く残っており、実用化に向けた研究段階である。本稿では、エクソソームを用いたドラッグデリバリーシステムの可能性、その利点や問題点などを述べる。
Hepatic Lipid Accumulation and Intracellular Cholesterol Crystal Formation in Hypercholesterolemic Diabetic Rats (1981)

Hypercholesterolemia, induced by feeding rats a diet of cholesterol and cholic acid, can be markedly accentuated by diabetes, resulting in serum cholesterol levels greater than 1000 mg/dl and extensive accumulate of cholesterol in the liver. To examine the cellular events of hepatic cholesterol accumulation in cholesterol-fed diabetic rats, we used a fixative containing digitonin and water-soluble embedment to preserve native lipid structures. Sprague Dawley male rats were made diabetic by intravenous injection of streptozotocin (45 mg/kg body weight) and fed a powdered diet containing 2% cholesterol, 10% lard and 1% cholic acid (CLC) for 3 wk. Non-diabetic rats were also fed CLC diet. Cholesterol, glucose and insulin levels were monitored for both groups. Livers were fixed by perfusion with 3% glutaraldehyde (30°C) and 0.2% digitonin in cacodylate buffer (1 hr), then by 1% O s O 4 in buffer for 2 hr. Staining was in 2% uranyl acetate and embedding in water soluble urea glutaraldehyde. Thin sections were stained with lead citrate and viewed in a Zeiss EM-9S.
Predictive current controller for a grid connected three level inverter with reactive power control (2010)

This paper presents a predictive method of current control for grid connected three level neutral point clamped inverter in synchronous rotating frame. The predictive current controller calculates the inverter voltage required to force the load current to follow the reference. To validate the performance the computational time has been compared with DSP processor TMS320C240 and the maximum permissible sampling frequency is calculated. Transient performance of the controller is analyzed. The proposed controller also validates the neutral voltage balancing and reactive power control. Three phase balanced current injection to the grid with reactive power control has been attained. The decoupled control over active and reactive power has been achieved through the variation of synchronously rotating two axis currents.
HCl-Formic In-Situ Gelled Acid for Carbonate Acidizing: Core Flood and Reaction Rate Study (2011)

Abstract Hydrochloric acid has been extensively used for well stimulation in matrix acidizing and acid fracturing. However, high reaction rate and severity of corrosion problems, especially for wells completed with Cr-based tubulars, limit the usage of the straight acid, especially for deep gas reservoirs. In-situ gelled acids are used to reduce leak-off rate and enhance acid diversion, while mixing organic acids with regular hydrochloric acid reduces the strength of HCl and increases the acid penetration. Literature review shows that very few numbers of studies have addressed the systems that utilize both advantages. The objective of this work is to investigate the effect of formic acid on the performance of the live and the spent in-situ gelled formic-HCl through viscosity measurements and core flood studies and to measure the rate of reaction of different formic-HCl in-situ gelled acid mixtures with calcite. Pink Desert limestone was utilized for the core flood and the reaction rate experiments. The core flood study was conducted at 250°F using two different rates of injection (2 and 10 cm3/min) and the rotating disk reactor was used to perform the reaction rate study. The effect of formic acid concentration, disk rotational speed, and zirconium cross-linking additive on the reaction rate was examined at 250°F. The formic acid concentration ranged from 0 to 6.31 wt% and the HCl concentration ranged from 0 to 5 wt.%. The core flood results showed that in this acid concentration range with the selected polymer and zirconium cross linking agent, the in-situ formic-HCl acid is behaving more like gelled acid. Increasing the formic acid concentration decreases the reaction rate with calcite at both low and high disk rotational speed. This was confirmed by the viscosity measurements, which showed that increasing the formic acid concentration increased the live acid viscosity and decreased the viscosity of the spent acid.
Reliability of logP Predictions Based on Calculated Molecular Descriptors: A Critical Review (2002)

Correct QSAR analysis requires reliable measured or calculated logP values, being logP the most frequently utilized and most important physico-chemical parameter in such studies. Since the publication of theoretical fundamentals of logP prediction, many commercial software solutions are available. These programs are all based on experimental data of huge databases therefore the predicted logP values are mostly acceptable - especially for known structures and their derivatives. In this study we critically reviewed the published methods and compared the predictive power of commercial softwares (CLOGP, KOWWIN, SciLogP/ULTRA) to each other and to our recently developed automatic QS(P)AR program. We have selected a very diverse set of 625 known drugs (98%) and drug-like molecules with experimentally validated logP values. We have collected 78 reported "outliers" as well, which could not be predicted by the "traditional" methods. We used these data in the model building and validation. Finally, we used an external validation set of compounds missing from public databases. We emphasized the importance of data quality, descriptor calculation and selection, and presented a general, reliable descriptor selection and validation technique for such kind of studies. Our method is based on the strictest mathematical and statistical rules, fully automatic and after the initial settings there is no option for user intervention. Three approaches were applied: multiple linear regression, partial least squares analysis and artificial neural network. LogP predictions with a multiple linear regression model showed acceptable accuracy for new compounds therefore it can be used for "in-silico-screening" and/or planning virtual/combinatorial libraries.
Capacity and Trainability in Recurrent Neural Networks (2016)

Two potential bottlenecks on the expressiveness of recurrent neural networks (RNNs) are their ability to store information about the task in their parameters, and to store information about the input history in their units. We show experimentally that all common RNN architectures achieve nearly the same per-task and per-unit capacity bounds with careful training, for a variety of tasks and stacking depths. They can store an amount of task information which is linear in the number of parameters, and is approximately 5 bits per parameter. They can additionally store approximately one real number from their input history per hidden unit. We further find that for several tasks it is the per-task parameter capacity bound that determines performance. These results suggest that many previous results comparing RNN architectures are driven primarily by differences in training effectiveness, rather than differences in capacity. Supporting this observation, we compare training difficulty for several architectures, and show that vanilla RNNs are far more difficult to train, yet have slightly higher capacity. Finally, we propose two novel RNN architectures, one of which is easier to train than the LSTM or GRU for deeply stacked architectures.
Polarization field crossover in semi‐polar InGaN/GaN single quantum wells (2010)

Abstract We present an electroreflectance study of the polarization field in semi‐polar (10 $ \bar 1\bar 1 $ ) and (11 $ \bar 2 $ 2) oriented InGaN quantum wells (QW). For the ( $ \bar 1\bar 1 $ ) sample, the flat‐QW condition (the electric field in the QW is zero) is at a reverse bias voltage. For the (11 $ \bar 2 $ 2) sample, the flat‐QW condition is at a forward bias voltage larger than the turn on voltage of the diode. However, the flat‐barrier condition (the electric field in the barrier region is zero) is at a forward bias voltage less than the turn on voltage of the diode. The flat‐QW condition and the flat‐barrier condition are determined by examining the zero‐crossing and the Franz‐Keldysh oscillations in the electroreflectance signal for (10 $ \bar 1\bar 1 $ ) and (11 $ \bar 2 $ 2) InGaN QWs, respectively. From the corresponding bias voltages, we deduce the polarization field in the QWs and conclude that in the semi‐polar InGaN/GaN QW there is a crossover angle between the polar and non‐polar orientations where the polarization field vanishes. (© 2010 WILEY‐VCH Verlag GmbH &amp; Co. KGaA, Weinheim)
Abstract 1207: In Vivo Molecular Imaging Links Macrophage Burden and Microcalcifications in Early Atherosclerosis (2007)

Backround Microcalcifications may provoke plaque rupture leading to acute cardiovascular events. However, the mechanisms that initiate arterial calcification remain obscure. Methods and Results We tested the hypothesis that inflammation promotes osteogenesis in atherosclerotic plaques using in vivo molecular imaging in apoE −/− mice (n=40). A bisphosphonate-derivatized near-infrared fluorescent (NIRF) imaging agent (750 nm) visualized osteogenic activity that was otherwise undetectable by X-ray computed tomography. Flow cytometry validated target cells as osteoblasts. A spectrally distinct NIRF nanoparticle (680 nm) was coinjected to simultaneously image macrophages. Fluorescence reflectance mapping associated osteogenic activity with inflammation in aortas of apoE −/− mice. Intravital dual-channel fluorescent microscopy further monitored osteogenic changes in carotid plaques at 20 and 30 weeks of age and revealed that both macrophage burden and osteogenesis concomitantly increased during plaque progression and decreased after statin treatment (Figure ) . Fluorescence microscopy on cryosections colocalized NIRF osteogenic signals with alkaline phosphatase activity, bone-regulating proteins and hydroxyapatite nanocrystals detected by electron microscopy, while von Kossa staining showed no evidence of calcification. Real-time RT-PCR revealed that macrophage-conditioned media induced alkaline phosphatase mRNA expression in vascular smooth muscle cells. Conclusion This serial in vivo study detects osteogenic activity within macrophage-rich plaques, offering a cellular resolution tool to identify preclinical microcalcifications.
Present and prospect of plant metabolomics (2010)

식물 대사체 (plant metabolomics) 연구는 식물 세포 및 조직에 존재하는 모든 대사산물의 시간적, 공간적 변화를 추적 조사함으로써 식물의 복잡한 생리 현상을 총체적으로 이해하는 연구이다. 이와 같은 식물 대사체 연구는 최근 개발이 이루어지고 있는 여러 오믹스 연구 분야의 하나로 시스템생물학의 한 분야이다. 식물 대사체 연구는 시료로부터 순수 화합물 또는 복합물을 정제하거나 또는 정제가 이루어지지 않은 혼합액으로부터 대사체 스펙트럼 정보를 확보하여 분석이 이루어지므로 추출액 제조 및 얻어진 대사체 데이터의 분석과정의 표준화가 필수적으로 이루어져야 한다. 이는 대사체 분석 결과의 해상도 및 재현성의 확보의 핵심 요소 이다. 식물 대사체 연구는 기능유전체학의 연구 수단은 물론 식물의 종, 품종, 더 나아가 GM 식물의 식별, 대사조절 기작 규명, 유용물질 생산, 식물의 외부 환경 스트레스 요인에 대한 다양한 생리적 반응 이해 등 다양한 연구 분야에서 활용이 이루어지고 있다. 최근 식물 대사체 연구는 모델식물(벼, 애기장대)의 유전체 정보와 연계하여 돌연변이주의 분석을 통해 유전자의 기능 정의 수단으로 활용되고 있다. 따라서 향후 유전체 정보와 대사체 정보의 연계를 통해 복잡한 대사경로 규명이나 다양한 생리 현상 해석 연구가 더욱 활발하게 진행될 것으로 전망된다. Plant metabolomics is a research field for identifying all of the metabolites found in a certain plant cell, tissue, organ, or whole plant in a given time and conditions and for studying changes in metabolic profiling as time goes or conditions change. Metabolomics is one of the most recently developed omics for holistic approach to biology and is a kind of systems biology. Metabolomics or metabolite fingerprinting techniques usually involves collecting spectra of crude solvent extracts without purification and separation of pure compounds or not in standardized conditions. Therefore, that requires a high degree of reproducibility, which can be achieved by using a standardized method for sample preparation and data acquisition and analysis. In plant biology, metabolomics is applied for various research fields including rapid discrimination between plant species, cultivar and GM plants, metabolic evaluation of commercial food stocks and medicinal herbs, understanding various physiological, stress responses, and determination of gene functions. Recently, plant metabolomics is applied for characterization of gene function often in combination with transcriptomics by analyzing tagged mutants of the model plants of Arabidopsis and rice. The use of plant metabolomics combined by transcriptomics in functional genomics will be the challenge for the coming year. This review paper attempted to introduce current status and prospects of plant metabolomics research.
Immunochromatographic assays for COVID-19 epidemiological screening: our experience (2020)

In March 2020, the World Health Organization (WHO) declared a pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Due to the absence of effective treatment or biomedical prevention, understanding potential post infection immunity has important implications for epidemiologic assessments. For this reason, increasing number of in vitro diagnostic companies are developing serological assays to detect antibodies against SARS-CoV-2, but most of them lack the validation by third parties in relation to their quality, limiting their usefulness. We submitted to serological screening by two different immunochromatographic (IC) rapid testing for detection of IgG and IgM against SARS-CoV-2, 151 asymptomatic or minimally symptomatic healthcare workers previously tested positive for SARS-CoV-2 RT-PCR in order to evaluate the performance of rapid assays. Results showed discrepancies between molecular and IC results, and an inconsistency of immunoglobulins positivity patterns when compared to ELISA/CLIA results, highlighting the absolute necessity of assays performance validation before their marketing and use, in order to avoid errors in the results evaluation at both clinical and epidemiological level.