gebhart/patrepeval huggingface dataset:
Systematic analysis of microRNAs targeting the androgen receptor in prostate cancer cells (2010)

Androgen receptor (AR) is expressed in all stages of prostate cancer progression, including in castration-resistant tumors. Eliminating AR function continues to represent a focus of therapeutic investigation, but AR regulatory mechanisms remain poorly understood. To systematically characterize mechanisms involving microRNAs (miRNAs), we conducted a gain-of function screen of 1129 miRNA molecules in a panel of human prostate cancer cell lines and quantified changes in AR protein content using protein lysate microarrays. In this way, we defined 71 unique miRNAs that influenced the level of AR in human prostate cancer cells. RNA sequencing data revealed that the 3'UTR of AR (and other genes) is much longer than currently used in miRNA target prediction programs. Our own analyses predicted that most of the miRNA regulation of AR would target an extended 6 kb 3'UTR. 3'UTR-binding assays validated 13 miRNAs that are able to regulate this long AR 3'UTR (miR-135b, miR-185, miR-297, miR-299-3p, miR-34a, miR-34c, miR-371-3p, miR-421, miR-449a, miR-449b, miR-634, miR-654-5p, and miR-9). Fifteen AR downregulating miRNAs decreased androgen-induced proliferation of prostate cancer cells. In particular, analysis of clinical prostate cancers confirmed a negative correlation of miR-34a and miR-34c expression with AR levels. Our findings establish that miRNAs interacting with the long 3'UTR of the AR gene are important regulators of AR protein levels, with implications for developing new therapeutic strategies to inhibit AR function and androgen-dependent cell growth.
Arterial Embolisms and Thrombosis in Upper Extremity Ischemia (2015)

At our center, embolism was found to be the most common etiology for UEI followed by thrombosis and stenosis. Patients presented with embolism were older, were more likely to undergo thromboembolectomy, and had higher rates of hypertension and longer hospital stays.
Recombinant Modified Vaccinia Virus Ankara (MVA) Expressing Wild-Type Human p53 Induces Specific Antitumor CTL Expansion (2011)

The p53 gene product is an attractive target for tumor immunotherapy. The present study aims to understand the potential of MVAp53 vaccine to induce expansion of p53-specific cytotoxic T lymphocyte ex vivo in cancer patients. The result indicated that 14 of 23 cancer patients demonstrated p53-specific IFN-γ production, degranulation, cell proliferation, and lysis of p53 overexpressed human tumor cell lines. These experiments show that MVAp53 stimulation has the potential to induce the expansion of p53-specific cytotoxic T lymphocyte from the memory T cell repertoire. The data suggest that MVAp53 vaccine is an ideal candidate for cancer immunotherapy.
High-Speed Signaling Interconnects Using Flat-Surface and Low-Dielectric-Loss Resin (2006)

配線の高精度化, 信号の高速化に伴い, 各種パッケージやプリント配線板の設計精度の向上, 製造精度の向上, 材料の特性改善およびこれらの協調が求められている。特に高周波領域における損失を削減するためには, 表面粗化を行わずに銅めっき膜を密着させることが必要である。これらに対し筆者らは, 表皮効果を考慮したRLGCモデルを用いることによる設計精度の向上と, 表面粗化を行わずに良好な銅めっき膜の密着 (9.2N/cm) を確保することのできるビルドアップ用低誘電率 (εr=2.75) ・低損失 (tanδ=0.0099) 樹脂の開発を行い, これらの検証を行った。37μm厚の樹脂フィルムを用いて50Ωのマイクロストリップライン (配線幅88μm, 配線厚15μm) を作成し伝播特性の計測を行った結果, 10GHzにおいて従来 (εr=3.4, tanδ=0.022, 配線幅77μm, 配線厚15μm) の約1/2である0.37dB/Cmの伝送損失を達成した。さらに, 表皮効果を考慮したRLGCモデルがよく一致することが確認され, これを用いて損失の内訳を算出した結果, 誘電体内での損失と配線金属での損失の両者を効果的に低減することができることが実証された。
Orally Absorbed Derivatives of the β-Lactamase Inhibitor Avibactam. Design of Novel Prodrugs of Sulfate Containing Drugs (2018)

Only one FDA-approved β-lactamase inhibitor has ever been orally available: clavulanic acid, approved in 1984. Avibactam, approved by FDA in 2015, is the first of a new class of BLIs called diazabicyclooctanes, or "DBOs". This class has much broader coverage than clavulanic acid but can only be administered by intravenous injection. Herein, we describe the synthesis and testing of the first approved BLI to be rendered orally bioavailable since clavulanic acid (1984).
Quantification of acute myocardial injury by ShMOLLI T1-Mapping, T2-weighted and late gadolinium imaging in patients presenting with chest pain, positive troponins and non-obstructive coronary arteries (2011)

To assess the ability of ShMOLLI T1-mapping in detecting acute myocardial injury in patients with chest pain, positive troponins and non-obstructive coronary arteries.
Post-translational Processing and Turnover Kinetics of Presynaptically Targeted Amyloid Precursor Superfamily Proteins in the Central Nervous System (1998)

The amyloid precursor superfamily is composed of three highly conserved transmembrane glycoproteins, the amyloid precursor protein (APP) and amyloid precursor-like proteins 1 and 2 (APLP1, APLP2), whose functions are unknown. Proteolytic cleavage of APP yields the betaA4 peptide, the major component of cerebral amyloid in Alzheimer's disease. Here we show that five post-translationally modified, full-length species of APP and APLP2 (but not APLP1) arrive at the mature presynaptic terminal in the fastest wave of axonal transport and are subsequently rapidly cleared (mean half-life of 3.5 h). Rapid turnover of presynaptic APP and APLP2 occurs independently of visual activity. Turnover of the most rapidly arriving APP species was accompanied by a delayed accumulation of a 120-kDa, APP fragment lacking the C terminus, consistent with presynaptic APP turnover via constitutive proteolysis. Turnover of APLP2 was not accompanied by detectable APLP2 fragment peptides, suggesting either that APLP2 either is more rapidly degraded than is APP or is retrogradely transported shortly after reaching the terminus. A single 150-kDa APLP2 species containing the Kunitz protease inhibitor domain is the major amyloid precursor superfamily protein transported to the presynapse. Presynaptic APP and APLP2 are sialylated and N- and O-glycosylated, and some also carry chondroitin sulfate glycosaminoglycan and/or dermatan sulfate glycosaminoglycan. The rapid kinetics for turnover of APP and APLP2 predict a sensitive balance of synthesis, transport, and elimination rates that may be critical to normal neuronal functions and metabolic fates of these proteins.
Co-delivery of gemcitabine and paclitaxel plus NanoCpG empowers chemoimmunotherapy of postoperative “cold” triple-negative breast cancer (2023)

Triple-negative breast cancer (TNBC) due to lack of clear target and notorious "cold" tumor microenvironment (TME) is one of the most intractable and lethal malignancies. Tuning "cold" TME into "hot" becomes an emerging therapeutic strategy to TNBC. Herewith, we report that integrin-targeting micellar gemcitabine and paclitaxel (ATN-mG/P, ATN sequence: Ac-PhScNK-NH<sub>2</sub>) cooperating with polymersomal CpG (NanoCpG) effectively "heated up" and treated TNBC. ATN-mG/P exhibited greatly boosted apoptotic activity in 4T1 cells, induced potent immunogenic cell death (ICD), and efficiently stimulated maturation of bone marrow-derived dendritic cells (BMDCs). Remarkably, in a postoperative TNBC model, ATN-mG/P combining with NanoCpG promoted strong anti-cancer immune responses, showing a greatly augmented proportion of mature DCs and CD8<sup>+</sup> T cells while reduced immune-suppressive myeloid-derived suppressor cells (MDSCs) and regulatory T cells (T<sub>reg</sub>), which led to complete inhibition of lung metastasis and 60% mice tumor-free. The co-delivery of gemcitabine and paclitaxel at desired ratio in combination with NanoCpG provides a unique platform for potent chemoimmunotherapy of "cold" tumors like TNBC.
Influence of carbon-based winding on maximum torque and specific torque designs of SPM machines (2021)

The article deals with optimal design of surface-mounted permanent-magnet machines with carbon-based conductors. Owing to its high electrical and thermal conductivity, as well as low mass density, carbon-based conductors could be a break-through technology in achieving more power-dense electric machines. Authors analytically optimize machines with different winding parameters for maximum torque and specific torque taking into account both electromagnetic and thermal phenomena. The optimized machines are compared to machines with copper winding and improvements of up to 50% in torque density are obtained.
Citrate-Capped Gold Nanoparticle Electrophoretic Heat Production in Response to a Time-Varying Radio-Frequency Electric Field (2012)

The evaluation of heat production from gold nanoparticles (AuNPs) irradiated with radiofrequency (RF) energy has been problematic due to Joule heating of their background ionic buffer suspensions. Insights into the physical heating mechanism of nanomaterials under RF excitations must be obtained if they are to have applications in fields such as nanoparticle-targeted hyperthermia for cancer therapy. By developing a purification protocol which allows for highly-stable and concentrated solutions of citrate-capped AuNPs to be suspended in high-resistivity water, we show herein, for the first time, that heat production is only evident for AuNPs of diameters ≤ 10 nm, indicating a unique size-dependent heating behavior not previously observed. Heat production has also shown to be linearly dependent on both AuNP concentration and total surface area, and severely attenuated upon AuNP aggregation. These relationships have been further validated using permittivity analysis across a frequency range of 10 MHz to 3 GHz, as well as static conductivity measurements. Theoretical evaluations suggest that the heating mechanism can be modeled by the electrophoretic oscillation of charged AuNPs across finite length scales in response to a time-varying electric field. It is anticipated these results will assist future development of nanoparticle-assisted heat production by RF fields for applications such as targeted cancer hyperthermia.
Blue Organic Light-Emitting Diodes Based on Triphenylene Derivatives (2013)

A series of blue fluorescent emitters based on triphenylene derivatives were synthesized via the Diels-Alder reaction in moderate yields. The electronic absorption and emission characteristics of the new functional materials were affected by the nature of the substituent on the triphenylene nucleus. Multilayered OLEDs were fabricated with a device structure of: ITO/NPB (50 nm)/EML (30 nm)/Bphen (30 nm)/Liq (2.0 nm)/Al (100 nm). All devices showed efficient blue emissions. Among those, a device using 1 gives the best performances with a high brightness (978 cd <TEX>$m^{-2}$</TEX> at 8.0 V) and high efficiencies (a luminous efficiency of 0.80 cd/A, a power efficiency of 0.34 lm/W and an external quantum efficiency of 0.73% at 20 <TEX>$mA/cm^2$</TEX>). The peak wavelength of the electroluminescence was 455 nm with CIEx,y coordinates of (0.17, 0.14) at 8.0 V.
Detection of VAC‐β (annexin‐8) in human placenta (1992)

A 36 kDa calcium/phospholipid binding protein in human placenta was identified as VAC-beta (annexin-8) by a combination of immunological and peptide mapping analyses. The protein is a minor product in placenta, accounting for less than 1% of extracted annexins. From 150 g of tissue, only 100 micrograms of the protein was isolated. By anion-exchange chromatography on diethylaminoethyl-cellulose annexin-8 coeluted with annexin-3. By gel filtration, the protein chromatographed as a broad peak, where half the product eluted as a monomer and half eluted as a heterodimer that was associated with a 10 kDa subunit. The combination of annexin-8 being a minor component in standard annexin preparations and it co-eluting with annexin-3 by ion exchange chromatography are likely to account for the failure of other labs to characterize the product.
Dielectric and Magnetic Nonlinear Materials for NLTLs (2018)

Nonlinear transmission lines (NLTLs) have been the subject of several studies that have shown their suitability for a wide variety of applications as pulse compressor, phase shifter, frequency multiplier, pulsed radar, battlefield communication disruption and also as a high power microwave source, holding in this case an additional way to generate high-power radio frequency (RF) signals without using vacuum electronic tubes, which normally requires heating filament and bias power supplies. NLTLs designed for the RF generation use nonlinear dielectric and magnetic materials that are arranged to form a dispersive or continuous nonlinear medium. This paper presents a summary of the main research on the recent NLTLs development and provides an analysis of the influence of the nonlinear material characteristics on the performance of NLTLs, pointing out that there is a lack of nonlinear dielectric and magnetic materials that would allow the achievement of NLTLs with better RF conversion efficiency and the operation at higher frequencies even under adverse environmental conditions. The nonlinear dielectric and magnetic materials for NLTLs applications need to have characteristics such as highly nonlinear behavior, low losses, and thermal stability.
Reproducibility and optimization of in  vivo human diffusion‐weighted MRS of the corpus callosum at 3T and 7T (2015)

Diffusion-weighted MRS (DWS) of brain metabolites enables the study of cell-specific alterations in tissue microstructure by probing the diffusion of intracellular metabolites. In particular, the diffusion properties of neuronal N-acetylaspartate (NAA), typically co-measured with N-acetylaspartyl glutamate (NAAG) (NAA + NAAG = tNAA), have been shown to be sensitive to intraneuronal/axonal damage in pathologies such as stroke and multiple sclerosis. Lacking, so far, are empirical assessments of the reproducibility of DWS measures across time and subjects, as well as a systematic investigation of the optimal acquisition parameters for DWS experiments, both of which are sorely needed for clinical applications of the method. In this study, we acquired comprehensive single-volume DWS datasets of the human corpus callosum at 3 T and 7 T. We investigated the inter- and intra-subject variability of empirical and modeled diffusion properties of tNAA [D(avg) (tNAA) and D(model) (tNAA), respectively]. Subsequently, we used a jackknife-like resampling approach to explore the variance of these properties in partial data subsets reflecting different total scan durations. The coefficients of variation (C(V)) and repeatability coefficients (C(R)) for D(avg) (tNAA) and D(model) (tNAA) were calculated for both 3 T and 7 T, with overall lower variability in the 7 T results. Although this work is limited to the estimation of the diffusion properties in the corpus callosum, we show that a careful choice of diffusion-weighting conditions at both field strengths allows the accurate measurement of tNAA diffusion properties in clinically relevant experimental time. Based on the resampling results, we suggest optimized acquisition schemes of 13-min duration at 3T and 10-min duration at 7 T, whilst retaining low variability (C(V) ≈ 8%) for the tNAA diffusion measures. Power calculations for the estimation of D(model )(tNAA) and D(avg) (tNAA) based on the suggested schemes show that less than 21 subjects per group are sufficient for the detection of a 10% effect between two groups in case-control studies.
Analysis of defects in an electric and photonic double-layer substrate made by separation-by-implanted-oxygen three-dimensional sculpting (2006)

With a view to application to electric-device fabrication, the authors investigated a separation-by-implanted-oxygen (SIMOX) three-dimensional (3D) sculpting method [P. Koonath et al., Appl. Phys. Lett. 83, 4909 (2003)] that will integrate in three dimensions electric circuits and photonic circuits on a silicon-on-insulator substrate. The authors analyzed the defects in the first silicon layer for electric circuits and the second silicon layer for optical circuits made by SIMOX 3D sculpting. The authors found that a balance between an acceptable number of defects on the first silicon layer and the formation of a buried optical waveguide is achievable. The defect densities of the first silicon layer and the second silicon layer were 1.8×103∕cm2 and 5.0×107∕cm2, respectively.
Supplementary Figure 5 from Impaired IFN-α Production by Plasmacytoid Dendritic Cells Favors Regulatory T-cell Expansion That May Contribute to Breast Cancer Progression (2023)

&lt;p&gt;PDF file, 72K, FACS analysis of CD40, CD80, and CD86 on TApDC following 40 h-culture in IL-3 alone or in combination with flu, R848, CpG-A, and CpG-B.&lt;/p&gt;
Predicting appropriate shocks in patients with heart failure: Patient level meta‐analysis from SCD‐HeFT and MADIT II (2017)

In this meta-analysis of patient level data from MADIT-II and SCD-HeFT, higher NYHA class, lower LVEF, no beta-blocker therapy, and single chamber ICD (vs. dual chamber) were significant predictors of appropriate shocks.
Engineering Nanodisc Scaffold Proteins for Native Mass Spectrometry (2017)

Lipoprotein nanodiscs are ideally suited for native mass spectrometry because they provide a relatively monodisperse nanoscale lipid bilayer environment for delivering membrane proteins into the gas phase. However, native mass spectrometry of nanodiscs produces complex spectra that can be challenging to assign unambiguously. To simplify interpretation of nanodisc spectra, we engineered a series of mutant membrane scaffold proteins (MSP) that do not affect nanodisc formation but shift the masses of nanodiscs in a controllable way, eliminating isobaric interference from the lipids. Moreover, by mixing two different belts before assembly, the stoichiometry of MSP is encoded in the peak shape, which allows the stoichiometry to be assigned unambiguously from a single spectrum. Finally, we demonstrate the use of mixed belt nanodiscs with embedded membrane proteins to confirm the dissociation of MSP prior to desolvation.
Alterations of the cortical representation of the rat ventrum induced by nursing behavior (1994)

The representation of the surfaces of the trunk was mapped in detail in a series of anesthetized adult female rats to assess cortical representational changes that might be induced in the SI cortical field by a major natural source of a differentially heavy schedule of tactile inputs: the stimulation of the rat ventrum in nursing behavior. Controls included virgin rats and postpartum age-matched rats whose litters were removed on the day of birth. The SI representation of the ventral trunk skin of lactating rats was about 1.6 x larger than in matched postpartum nonlactating or virgin controls. The greatest representational change--about twofold--was for the nipple-bearing skin between the forelimbs and hindlimbs. Indeed, changes in SI representational territory for the middle third of the ventrum, a skin zone without nipples, were not significant. As a rule, the representation of the ventrum skin in lactating rats was at least as topographically ordered as was that reconstructed for nonlactating postpartum and virgin controls. Receptive fields (RFs) representing the ventrum skin in lactating females were about one-third the sizes of those recorded in matched nonlactating or virgin controls. RF size differences were again greater for the representation of the nipple-bearing skin in the anterior and posterior thirds of the ventrum than for the central third. Changes in RF sizes were roughly inversely related to changes in the cortical magnification of representation of the ventrum on the proportion of about 3:2. Interestingly, the glabrous nipple and areolar skin were only weakly represented--or not demonstrably represented--in the SI map of either lactating or control rats. These results indicate that there is a largely unstudied cortical neurology of nursing behavior. Major CNS changes are induced by this dramatic, episodic change in behaviorally important tactile inputs. In turn, input-induced changes presumably contribute to this emergent, rapidly evolving behavior.
Molybdenum Disulfide/Double‐Wall Carbon Nanotube Mixed‐Dimensional Heterostructures (2022)

Abstract Mixed‐dimensional heterostructures which combine materials with different dimensions have emerged to expand the scope and functionality of van der Waals heterostructures. Here, a direct synthesis method of molybdenum disulfide/double‐wall carbon nanotube (MoS 2 /DWCNT) mixed‐dimensional heterostructures by sulfurating a molten salt, Na 2 MoO 4 , on a substrate covered with a DWCNT film is reported. The synthesized heterostructures are comprehensively characterized and their stacking order is confirmed to be MoS 2 under the DWCNTs, although the DWCNT film is transferred on the substrate first. Moreover, field‐effect transistors based on the heterostructure are fabricated for photodetection, and an abnormal negative photoresponse is discovered due to the strong carrier transfer in the mixed‐dimensional heterostructures under light incidence. The MoS 2 /DWCNT heterostructure results provide a new approach for the synthesis and applications of mixed‐dimensional heterostructures.
A channel predictive proportional fair scheduling algorithm (2005)

There is growing interest in the area of cross-layer design. This paper addresses the problem of multi-user diversity scheduling together with channel prediction. Recent work demonstrates that the mobile radio channel can be linearly predicted, quite accurately, several milliseconds ahead in time for realistic Doppler spreads. We show how this information can be exploited in an original fashion to improve the so-called throughput-fairness trade-off in multiuser wireless fading channel scheduling. Simulations show that the proposed algorithm can give considerable throughput gains without compromising fairness. The current approach builds on the proportional fair scheduling technique but can also be generalized to other criteria.
Reliability of Recess-channel Gate Cell Transistor under Gate-Induced Drain Leakage Stress and Positive Bias Fowler-Nordheim Gate Stress (2006)

The reliability of recess-channel gate (RG) cell transistor under positive bias Fowler-Nordheim (F-N) gate stress and gate-induced drain leakage (GIDL) stress was investigated. RG cell transistor was found to be more degraded by the F-N gate stress than the GIDL stress due to the gate oxide thickness profile of the RG structure. The oxide thickness along the sidewall plane is a critical factor determining the reliability characteristics of RG cell transistor
Biodiversity of Thermophilic Prokaryotes with Hydrolytic Activities in Hot Springs of Uzon Caldera, Kamchatka (Russia) (2008)

Samples of water from the hot springs of Uzon Caldera with temperatures from 68 to 87 degrees C and pHs of 4.1 to 7.0, supplemented with proteinaceous (albumin, casein, or alpha- or beta-keratin) or carbohydrate (cellulose, carboxymethyl cellulose, chitin, or agarose) biological polymers, were filled with thermal water and incubated at the same sites, with the contents of the tubes freely accessible to the hydrothermal fluid. As a result, several enrichment cultures growing in situ on different polymeric substrates were obtained. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene fragments obtained after PCR with Bacteria-specific primers showed that the bacterial communities developing on carbohydrates included the genera Caldicellulosiruptor and Dictyoglomus and that those developing on proteins contained members of the Thermotogales order. DGGE analysis performed after PCR with Archaea- and Crenarchaeota-specific primers showed that archaea related to uncultured environmental clones, particularly those of the Crenarchaeota phylum, were present in both carbohydrate- and protein-degrading communities. Five isolates obtained from in situ enrichments or corresponding natural samples of water and sediments represented the bacterial genera Dictyoglomus and Caldanaerobacter as well as new archaea of the Crenarchaeota phylum. Thus, in situ enrichment and consequent isolation showed the diversity of thermophilic prokaryotes competing for biopolymers in microbial communities of terrestrial hot springs.