gebhart/patrepeval huggingface dataset:
Dendrimer-Modified Solid Supports: Nanostructured Materials with Potential Drug Allergy Diagnostic Applications (2012)

Complex functional materials consisting of bioactive molecules immobilized on solid supports present potential applications in biosensoring. Advances in the fabrication of these surface materials are of growing interest for antibody-based diagnosis. This work exploits dendrimers as versatile nanostructures for templating sensor surfaces and the critical role of the immobilization protocol in the solid supports cellulose and zeolites, of organic and inorganic composition respectively. The fabrication and characterization, including the degree of functionalization and reproducibility, of different nanostructured materials are described. To validate the approach, the fabricated supports were further used as a solid phase for developing a radioimmunoassay to detect immunoglobulin E (IgE) specific to penicillin, the antibody involved in immediate allergy responses to this drug. The dendrimer-modified supports provide assays with significantly enhanced sensitivity, as well as increase the availability of biomolecules for specific interaction and minimize nonspecific adsorptions through appropriate functionalization protocols in each case. The manufacturing methodology involved the use of a long, flexible hydrophilic spacer in the cellulose materials, and a higher surface density of the immobilized dendrimers in the zeolite crystals. The ability of hybrid zeolite materials in such biosensing applications was evaluated for the first time. The assays were validated in human serum samples from patients allergic to penicillin and from non-allergic controls. The specificity and improved sensitivity of the dendrimer- modified supports make these strategies versatile for different bioactive molecules and could have significant implications for the quantification of a wide range of specific IgE antibodies and other biomolecules of diagnostic interest.
A Facile Mechanism for Recharging Li2O2 in Li–O2 Batteries (2013)

Li–air is a novel battery technology with the potential to offer very high specific energy, but which currently suffers from a large charging overpotential and low power density. In this work, we use ab initio calculations to demonstrate that a facile mechanism for recharging Li2O2 exists. Rather than the direct decomposition pathway of Li2O2 into Li and O2 suggested by equilibrium thermodynamics, we find an alternative reaction pathway based on topotactic delithiation of Li2O2 to form off-stoichiometric Li2–xO2 compounds akin to the charging mechanism in typical Li-ion intercalation electrodes. The low formation energy of bulk Li2–xO2 phases confirms that this topotactic delithiation mechanism is rendered accessible at relatively small overpotentials of ∼0.3–0.4 V and is likely to be kinetically favored over Li2O2 decomposition. Our findings indicate that at the Li2O2 particle level there are no obstacles to increase the current density, and point to an exciting opportunity to create fast charging Li–air systems.
Oxygen tension at the subcutaneous implantation site of glucose sensors. (1989)

To elucidate potential influences of the average tissue pO2 on the function of implanted glucose sensors, non-miniaturized polarographic oxygen electrodes and glucose oxidase/H2O2 glucose electrodes were implanted in the subcutaneous tissue of spontaneously breathing normal and diabetic dogs. There was no appreciable run-in phenomenon of oxygen sensors but normally a pronounced initial decrease in current after implantation of glucose sensors. The subcutaneous pO2 amounted to an average of 7 kPa in air-breathing animals with no difference between normal and insulin-dependent diabetic dogs. It showed oscillations of approximately +/- 2 kPa but the mean was stable over the maximum duration of experiments of 16 h. Induced alterations of tissue pO2 between less than 2 and greater than 20 kPa (as verified by measurements of arterial pO2) were not followed by alterations in the current of nearby implanted glucose sensors. It is concluded that the frequently observed instabilities and losses in sensitivity of the system "implanted glucose sensor in situ + tissue glucose compartment" are not caused by alterations in tissue pO2.
Adaptive neural network management system (2023)

A method and computer system for managing a neural network. Data is sent into an input layer in a portion of layers of nodes in the neural network. The data moves on an encode path through the portion such that an output layer in the portion outputs encoded data. The encoded data is sent into the output layer on a decode path through the portion back to the input layer to obtain a reconstruction of the data by the input layer. A determination is made as to whether an undesired amount of error has occurred in the output layer based on the data sent into the input layer and the reconstruction of the data. A number of new nodes is added to the output layer when a determination is present that the undesired amount of the error occurred, enabling reducing the error using the number of the new nodes.
Fat-Suppression Techniques for 3-T MR Imaging of the Musculoskeletal System (2014)

Fat suppression is an important technique in musculoskeletal imaging to improve the visibility of bone-marrow lesions; evaluate fat in soft-tissue masses; optimize the contrast-to-noise ratio in magnetic resonance (MR) arthrography; better define lesions after administration of contrast material; and avoid chemical shift artifacts, primarily at 3-T MR imaging. High-field-strength (eg, 3-T) MR imaging has specific technical characteristics compared with lower-field-strength MR imaging that influence the use and outcome of various fat-suppression techniques. The most commonly used fat-suppression techniques for musculoskeletal 3-T MR imaging include chemical shift (spectral) selective (CHESS) fat saturation, inversion recovery pulse sequences (eg, short inversion time inversion recovery [STIR]), hybrid pulse sequences with spectral and inversion-recovery (eg, spectral adiabatic inversion recovery and spectral attenuated inversion recovery [SPAIR]), spatial-spectral pulse sequences (ie, water excitation), and the Dixon techniques. Understanding the different fat-suppression options allows radiologists to adopt the most appropriate technique for their clinical practice.
Soluble P-selectin moderates complement-dependent reperfusion injury of ischemic skeletal muscle (2000)

P-selectin is an adhesion molecule expressed on activated endothelial and platelet surfaces. The function of the short consensus repeats (SCRs) of P-selectin, homologous with the SCRs of complement regulatory proteins is largely unknown. In a model of murine hindlimb ischemia where local reperfusion injury is partly mediated by IgM natural antibody and classical complement pathway activation, we hypothesized that human soluble P-selectin (sP-sel) would moderate the complement component of the inflammatory response. Infusion of sP-sel supernatant or purified (p) sP-sel prepared from activated human platelets, reduced ischemic muscle vascular permeability by 48% and 43%, respectively, following reperfusion. Hindlimb immunohistochemistry demonstrated negligible C3 staining colocalized with IgM in these groups compared with intense staining in the untreated injured mice. In vitro studies of mouse serum complement hemolytic activity showed that psP-sel inhibited the classical but not alternative complement pathway. Flow cytometry demonstrated that psP-sel inhibited C1q adherence to sensitized red blood cells. From these data we conclude that sP-sel moderates skeletal muscle reperfusion injury by inhibition of the classical complement pathway.
Reversible Insulin Self-Assembly under Carbohydrate Control (2005)

Insulin with built-in pairs of boronates and polyols can produce soluble high molecular weight self-assemblies under control by carbohydrates. The illustrated principle has potential utility for general protein and peptide protraction and controlled drug release.
<title>Hard-disk actuators for mini-teleoperation</title> (1995)

Hard disk drives have evolved rapidly with computer miniaturization into highly compact and integrated electromechanical systems. Hard drives contain many precision mechanical parts which may prove useful in the design of small precision robots. The advantages of parts taken from hard disks include low cost, miniaturization, high quality, and for some applications, cleanliness. We report the results of engineering tests on flat coil head positioning actuators taken from hard drives of sizes ranging from 5.25' to 1.8' media diameter. We also perform a simple analysis which suggests that requirements for torque per unit mass are lower for small robot arms. The results suggest ways that hard disk actuators can be utilized in mini robotic designs and points the way towards improved versions of these designs for robotic purposes.
A copolymer coating for improving bio-functionalization and reducing nonspecific biofouling on neural probes (2016)

Frontiers Events is a rapidly growing calendar management system dedicated to the scheduling of academic events. This includes announcements and invitations, participant listings and search functionality, abstract handling and publication, related events and post-event exchanges. Whether an organizer or participant, make your event a Frontiers Event!
Silk‐Based Fine Dust Filters for Air Filtration (2017)

Abstract State‐of‐the‐art air filter devices have been well‐characterized and optimized for particle deposition of a huge range of particle sizes. However, sustainability of the employed filter materials is usually neglected. In this study, several polymers (recombinant spider silk, recombinant lacewing silk, poly(lactic acid), and poly(ethylene oxide)) are electrospun into fine dust filter layers to evaluate relevant filter parameters such as particle deposition, air permeability, and pressure drop for sub‐micrometer fiber materials. Filters comprising silk proteins show a much lower demand on fine‐dust‐layer material (40–210 mg m −2 ) in comparison to that of conventional filters (50 g m −2 ). Filters equipped with silk meshes with fiber diameters in the sub‐micrometer regime (&lt;250 nm) show improved filter qualities in comparison to that of commercially available air filter devices (with melt blown fibers in the &gt;20 µm regime). In particular, sub‐micrometer particles (0.2–1 µm) are filtered more efficiently by the silk‐based filters due to interception and impaction effects yielding a low pressure drop, and therefore these filters show an improved air permeability and lowered pressure drop of, for example, a vacuum cleaner bag in which they can be employed.
Thalidezine, a novel AMPK activator, eliminates apoptosis-resistant cancer cells through energy-mediated autophagic cell death (2017)

Cancers illustrating resistance towards apoptosis is one of the main factors causing clinical failure of conventional chemotherapy. Innovative therapeutic methods which can overcome the non-apoptotic phenotype are needed. The AMP-activated protein kinase (AMPK) is the central regulator of cellular energy homeostasis, metabolism, and autophagy. Our previous study showed that the identified natural AMPK activator is able to overcome apoptosis-resistant cancer via autophagic cell death. Therefore, AMPK is an ideal pharmaceutical target for chemoresistant cancers. Here, we unravelled that the bisbenzylisoquinoline alkaloid thalidezine is a novel direct AMPK activator by using biolayer interferometry analysis and AMPK kinase assays. The quantification of autophagic EGFP-LC3 puncta demonstrated that thalidezine increased autophagic flux in HeLa cancer cells. In addition, metabolic stress assay confirmed that thalidezine altered the energy status of our cellular model. Remarkably, thalidezine-induced autophagic cell death in HeLa or apoptosis-resistant DLD-1 BAX-BAK DKO cancer cells was abolished by addition of autophagy inhibitor (3-MA) and AMPK inhibitor (compound C). The mechanistic role of autophagic cell death in resistant cancer cells was further supported through the genetic removal of autophagic gene7 (Atg7). Overall, thalidezine is a novel AMPK activator which has great potential to be further developed into a safe and effective intervention for apoptosis- or multidrug-resistant cancers.
Protecting Human Life by Detecting Toxic Gases in Underground Drainage and Mining using IoT (2019)

I. INTRODUCTION Drainage system plays a very important role in big cities where millions of people live .In order to maintain its proper function drainage conditions should be monitored. Where, all areas does not have drainage monitoring team and it leads to irregular monitoring of the condition of drainage. The irregular monitoring has the contribution on blocking the drainage that results in the salutation which leads to flood in neighborhood. Manual monitoring is also not possible all the time. It involves a lot of man power where they are able to record limited number of report with low accuracy. The main problem arises in such drainages that leads to the serious issues to the daily routine of the city. Problems like blockages due to waste material and also sudden increase in the level of water and as well as various harmful gases can be produced if proper cleaning actions are not taken at the right time. Where today's drainage system is not digitalized because it is hard to know where blockage is particularly occurring in the location. And also sometimes because of the waste in those drainage lines can produce various gases like methane (CH4), carbon monoxide (CO), etc which are harmful and can cause serious problem if inhaled by human's in large amount and these problems are generally faced by the drainage workers due to which death can occurs. Also we don't get early alerts of the blockage or rise in amount of those gases or the increase in water level. Hence detection and repairing of the blockage becomes time consuming and hectic. Every node consist the data processing (Microcontroller, e.g. -Arduino) memory (program, data, flash memory), power supply system and involves two or more sensors. This system have a higher level of precision than wired network system with respect to cost; flexibleness and reliability are expected to replace hybrid or combined technology. According to an estimation we made till 2019, at least 22327 men die in India every year because of inhaling the poisonous gas while doing the drainage related work and also two to three workers must be dying every day inside manholes across India. 1) It just took a few minutes for six people to fall dead, one after another, when noxious gas emanating from a septic tank in sriperambudur which is 40km from Chennai on Tuesday(26-03-2019). 2) And also two staffers where dead while cleaning restaurant's sewage treatment plant in the capital of India on Saturday (23-03-2019) and many such incidents.
57&#x2013;64 GHz seven-pole bandpass filter Substrate Integrated Waveguide (SIW) in LTCC (2011)

This paper presents the design and characterization of a Substrate Integrated Waveguide (SIW) filter, with a large bandwidth of 7 GHz, designed to cover the unlicensed frequency band, 57-64 GHz. The filter, a seven-pole Chebyshev is integrated in Low-Temperature Co-fired Ceramic (LTCC). It is characterized by a measured bandwidth of 11.8% around a central frequency of 59.1 GHz. It presents a low passband insertion loss of 2.4 dB and high stop band insertion loss in the lower and higher band, respectively 50 dB and 30 dB. The filter has a compact size of 9450 × 2100 × 200 μm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sup> .
Transient modeling of viscosity (2017)

Capillary and parallel plate rheological characterization was conducted for a low‐density polyethylene. In contrast with conventional rheological analysis, steady conditions were not assumed. Transient data, with time steps between 0.0001 and 0.2 s, were analyzed with a nonlinear, viscoelastic constitutive model in which the relaxation time was modeled as a function of the applied stress. The fit model explained more than 99% of the observed transient variation in the capillary and parallel plate rheometers. The model coefficients for the capillary and parallel plate were compared directly to conventional linear viscoelastic analysis of the same parallel plate data. The results indicate that the described constitutive model closely predicts the observed viscoelastic behavior of the polymer melt tested in the parallel plate rheometer. Furthermore, the results indicate that the relaxation spectrum modeled with the transient analysis of the capillary rheological data correlate closely to the results predicted by the same transient analysis of parallel plate rheological data. The conclusion is that described constitutive modeling describes the viscoelastic behavior in both capillary and parallel plate rheometers. Moreover, the analysis and results suggest that the viscoelastic behavior of the polymer melt is a significant factor during the rheological characterization and the modeling of the transient response should be taken into consideration during rheological analysis to provide high fidelity models. POLYM. ENG. SCI., 57:1110–1118, 2017. © 2017 Society of Plastics Engineers
Peak satellite-to-earth data rates derived from measurements of a 20 Gbps bread-board modem (2011)

A prototype data link using a Ka-band space qualified, high efficiency 200 W TWT amplifier and a bread-board modem emulator were created to explore the feasibility of very high speed communications in satellite-to-earth applications. Experiments were conducted using a DVB-S2-like waveform with modifications to support up to 20 Gbps through the addition of 128 Quadrature Amplitude Modulation (QAM). Limited by the bandwidth of the amplifier, a constant peak symbol rate of 3.2 Giga-symbols/sec was selected and the modulation order was varied to explore what peak data rate might be supported by an RF link through this amplifier. Using 128-QAM, an implementation loss of 3 dB was observed at 20 Gbps, and the loss decreased as data rate or bandwidth were reduced. Building on this measured data, realistic link budget calculations were completed. Low-earth orbit (LEO) missions based on this TWTA with reasonable hardware assumptions and antenna sizing are found to be bandwidth-limited, rather than power-limited, making the spectral efficiency of 9/10-rate encoded 128-QAM very attractive. Assuming a bandwidth allocation of 1 GHz, these computations indicate that low earth orbit vehicles could achieve data rates up to 5 Gbps—an order of magnitude beyond the current state-of-practice, yet still within the processing power of a current FPGA-based software-defined modem. The measured performance results and a description of the experimental setup are presented to support these conclusions.
Tables of Glycosidase Inhibitors with Nitrogen in the Sugar Ring and Their Inhibitory Activities: Section 9 (1998)

This appendix contains section titled: Calystegines
Variable stiffness prosthetic foot based on rheology properties of shear thickening fluid (2020)

The unique rheological properties of discontinuously shear thickening fluids (STF) have been employed in various engineering applications in recent years. In a commercial aspect, this has most notably been body armor and protective equipment, but also specialized smart structures in damping and force-coupling applications. The topic of this work is the application of STF in an articulating prosthetic foot for adaptable force response. Connected in series and parallel to a spring system, a STF based element can be used to affect the force transfer within the system, and thereby, influence the stiffness of the prosthetic foot dynamically over the gait cycle. The device described, prototyped and tested in this work, is a STF filled piston/cylinder design. The objective is a velocity dependent force response over the translational motion. Ranging from dampened, compliant deflection at low velocity, to a more efficient force transfer (coupling) for energy storage and return in the spring system, at higher speed. The rapid viscosity increase in the STF at a critical shear rate is used to approach a stepwise force response, thereby enabling adaptive response of the foot for different load rates. The adaptive response results in a greater range of motion with easier rollover for slow movement, for instance standing up from a seated position and adaptation to inclined surfaces, without sacrificing the energy return favorable for normal walking.
Nucleophile recognition as an alternative inhibition mode for benzoic acid based carbonic anhydrase inhibitors (2012)

A series of hydroxybenzoic acid derivatives have shown inhibitory activity against carbonic anhydrase (CA). X-ray crystallography shows that these molecules inhibit not by binding the active site metal ion but by strong hydrogen bonding to the metal-bound water nucleophile. The binding mode observed for these molecules is distinct when compared to other non-metal-binding CA inhibitors.
Detection of Lignin Peroxidase and Xylanase by Immunocytochemical Labeling in Wood Decayed by Basidiomycetes (1989)

The white rot fungi used in this study caused two different forms of degradation. Phanerochaete chrysosporium, strain BKM-F-1767, and Phellinus pini caused a preferential removal of lignin from birch wood, whereas Trametes (Coriolus) versicolor caused a nonselective attack of all cell wall components. Use of polyclonal antisera to H8 lignin peroxidase and monoclonal antisera to H2 lignin peroxidase followed by immunogold labeling with protein A-gold or protein G-gold, respectively, showed lignin peroxidase extra-and intracellularly to fungal hyphae and within the delignified cell walls after 12 weeks of laboratory decay. Lignin peroxidase was localized at sites within the cell wall where electron-dense areas of the lignified cell wall layers remained. In wood decayed by Trametes versicolor, lignin peroxidase was located primarily along the surface of eroded cell walls. No lignin peroxidase was evident in brown-rotted wood, but slight labeling occurred within hyphal cells. Use of polyclonal antisera to xylanase followed by immunogold labeling showed intense labeling on fungal hyphae and surrounding slime layers and within the woody cell wall, where evidence of degradation was apparent. Colloidal-gold-labeled xylanase was prevalent in wood decayed by all fungi used in this study. Areas of the wood with early stages of cell wall decay had the greatest concentration of gold particles, while little labeling occurred in cells in advanced stages of decay by brown or white rot fungi.
Shedding of soluble glycoprotein 1 detected during acute Lassa virus infection in human subjects (2010)

During a narrow window following active infection with LASV, soluble GP1 can be detected in patient sera. This phenomenon parallels other VHF infection profiles, with the actual role of a soluble viral glycoprotein component in vivo remaining largely speculative. The expenditure of energy and cellular resources toward secretion of a critical protein during viral biogenesis without apparent specific function requires further investigation. Future studies will be aimed at systematically identifying the role of LASV sGP1 in the infection process and outcome in vitro and in vivo.
Activation of human immune cells by the oncolytic parvovirus H-1 combined with chemotherapeutic or targeted agents (2010)

Oncolytic viruses, such as autonomous parvoviruses (PV) and their therapeutic vectors are known to stimulate potent antitumoral immune responses in vitro and in vivo. The parvovirus H-1 efficiently infects and lyses human tumor cells, including human melanoma, hepatoma, colon- and gastric cancer cells. The aim of our research project was now to further evaluate the biologic and immunologic effects of H-1PV-induced tumor cell lysis (TCL) and its combination with chemotherapeutic and molecular targeted agents in two human melanoma models with adjacent characterized human cytotoxic T cells (CTL) and HLA-A2 restricted dendritic cells (DC).