gebhart/patrepeval huggingface dataset:
Evolution of the optimal catalytic systems for the oxidative dehydrogenation of ethane: The role of adsorption in the catalytic performance (2021)

Three samples that correspond to the evolution of optimal catalytic systems for the oxidative dehydrogenation of ethane have been synthesized and compared in terms of catalytic behavior and adsorption properties: (i) vanadium oxide supported on alumina, (ii) Sn-promoted NiO, and (iii) multicomponent MoVTeNbO with the M1 structure. The main difference in catalytic performance lies in the extent of the overoxidation of the ethylene formed, following the order VOx/Al2O3 > NiSnOx > MoVTeNb-M1. Accordingly, the selectivity to ethylene at medium and high ethane conversion follows the order MoVTeNb-M1 > NiSnOx > VOx/Al2O3. These results are confirmed by the relative reaction rates observed for the oxidation of ethane and the oxidation of ethylene. Microcalorimetry studies indicate that the heat of adsorption of both ethane and ethylene is the highest in the most selective MoVTeNb-M1 sample. Thus, the low olefin decomposition in the MoVTeNb-M1 catalyst is not due to weaker adsorption of ethylene but to the reduced ability of its active sites to activate ethylene. The same conclusion regarding the MoVTeNb-M1 catalyst can be drawn by FT-IR of adsorbed ethylene. On the other hand, NiSnOx active sites present a high overoxidation ability, as demonstrated by the notorious formation of oxygenated species, precursors of COx. However, the ethylene decomposition is rather mild because of the existence of many free Lewis sites not involved in the overoxidation reaction. In contrast, in the case of the VOx/Al2O3 catalyst, almost all active sites are involved in the oxidation path, so that the olefins decompose readily.
Improvement of Luminance and Luminous Efficacy of Mercury-Free, Flat Fluorescent Lamp by Optimizing Phosphor Profile (2010)

A highly efficient mercury-free fluorescent lamp (MFFL) was developed for an alternative to fluorescent lamps containing mercury. The lamp has a simple structure with two main electrodes running parallel to each other and an auxiliary electrode. In order to improve the luminance and luminous efficacy of MFFLs further, we optimized the thickness of rear and front phosphors on the inner surfaces of the lamp, and then adopted an uneven distribution profile for the rear phosphor to increase the surface area of the phosphor. The MFFL with the optimized phosphor profile showed a wide luminance range from 3315 to 17133 cd/m <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> with the corresponding luminous efficacy from 75.2 to 42.5 lm/W with the color coordinate around (0.32, 0.32) for daylight.
Effect of consuming a grape seed supplement with abundant phenolic compounds on the oxidative status of healthy human volunteers (2015)

This simple dietary intervention significantly reduced (33%) the urine redox potential, reflecting an overall increase in antioxidant status. Incorporation of plant-derived phenols in the diet may increase anti-oxidative status.
Evaluation of the iTClamp 50 in a human cadaver model of severe compressible bleeding (2014)

The iTClamp is effective at controlling fluid loss from open wounds within multiple compressible areas. The iTClamp does not occlude distal flow, and aside from small needle punctures, there was no other visible skin damage or skin tearing.
Delta-sigma-based transmitters: Advantages and disadvantages (2013)

Power efficiency is one of the most important parameters in designing communication systems, especially battery operated mobile terminals. In a typical transceiver, most of the power is dissipated in the power amplifier (PA) and consequently, it is very important to obtain the maximum efficiency from the PA. A PA operating in Class AB or B is at its maximum efficiency when it is driven by its maximum allowable input power [1]. In practice, the input signal of the PA usually has a varying envelope, and to avoid distortion the PA should not be driven to more than its maximum input saturating power. Unfortunately, this peak power of the input signal happens at very short periods, and most of the time the signal power is around its average power, which is much smaller than its peak power, meaning that, often, the PA works at much lower efficiencies than its maximum efficiency. The power difference is defined as the peak to average power ratio (PAPR) of the signal. For example, for a signal with 12 dB PAPR, a Class B PA would be driven with 12 dB power back-off from its peak input power, and at this power back-off, the efficiency of the PA will degrade from 78.5% to around 20% [1]. Unfortunately, by moving to high throughput modulation schemes, for example, quadrature amplitude modulations (QAMs) such as 16-QAM and 64-QAM mean that more envelope variation is needed to encode the information, and, consequently, lower efficiency is achieved.
Isolation of Daudi Cells with Reduced Sensitivity to Interferon. IV. Characterization of Clones with Altered Binding of Human Interferon Subspecies (1986)

Three clones of Daudi cells, DIF3, DIF8 and DIF9, have been isolated which possess specific interferon (IFN) receptors even though these cells are resistant to both the antiviral and antiproliferative actions of human IFN-alpha. Studies with 125I-labelled cloned human IFN-alpha subspecies showed that clone DIF3 bound IFN in a manner identical to that of the IFN-sensitive parental cells. The initial peak of binding observed 30 min after treatment of either Daudi or DIF3 cells at 37 degrees C with IFN-alpha 2 or IFN-alpha 8 was, however, either absent or much reduced when using DIF8 cells which are resistant to the antiproliferative action of IFN-alpha 2 and -alpha 8. Similarly, no initial peak of binding was observed after treatment of DIF9 cells with IFN-alpha 2 at 37 degrees C. In accord with its reduced biological activity, the binding of 125I-IFN-alpha 1 to either Daudi or DIF8 cells was considerably lower than that of either of the other two IFN-alpha subspecies and followed the form of a hyperbola without an initial transitory peak of binding. It is suggested that the phenotype of IFN resistance of DIF8 and DIF9 cells may be related to a defect in receptor activation whereas the same phenotype of DIF3 cells would appear to be unrelated to IFN binding.
Bottom-emitting 850 nm selectively oxidized VCSELs fabricated using wafer bonding (2002)

We report on bottom-emitting 850 nm selectively oxidized vertical cavity surface emitting lasers (VCSELs) that are wafer bonded to AlGaAs substrates. We employ a simple low temperature wafer bonding process that uses inert N/sub 2/ gas during the bonding anneal rather than H/sub 2/.
The Prostate Clinical Outlook (PCO) Classifier Application for Predicting Biochemical Recurrences in Patients Treated by Stereotactic Body Radiation Therapy (SBRT) (2018)

(1) Background: Prostate cancer risk classifiers have been used for predicting surgical and radiation therapy outcomes; however, a classifier for predicting biochemical recurrence (BCR) in patients undergoing stereotactic body radiation therapy (SBRT) is not available. We attempted to develop a model that creates a risk classifier to predict BCR in patients considering SBRT. (2) Methods: We studied the outcomes of 809 patients treated with SBRT between August 2007 and November 2016. We used Cox regression analysis with time to BCR as the outcome to develop a model that calculates a prostate clinical outlook (PCO) score based on age at diagnosis, clinical-radiological staging, and a modified risk level. We then created the PCO classifier application, which uses the model we created to categorize patients into risk groups based on multiple factors. We assessed the concordance index (c-index) to determine the accuracy of the PCO classifier application and compared the results to the D’Amico and Kattan nomogram classifications. (3) Results: The calculated PCO scores ranged from 0 to 156 points. The PCO classifier application categorized patients into three risk-groups, with 5-year BCR-free survival rates of 98.3% for low risk (n = 137), 95.4% for intermediate risk (n = 570), and 86.4% for high risk (n = 102). We demonstrated the improved prognostic power of the PCO classifier application, with a c-index of 0.75 (training set) and 0.67 (validation set); the c-index of the Kattan nomogram was 0.62 and 0.63, respectively, and that of the D’Amico classifier was 0.64 and 0.64, respectively. (4) Conclusions: The PCO classifier application is a predictive tool for employing readily available clinical parameters to stratify prostate cancer patients and to predict the probability of BCR after SBRT.
Abstract 104: ATF7IP does not alter the substrate specificity of the lysine methyltransferase SETDB1 (2015)

Abstract The histone methyltransferase (HMT) SETDB1 is a strong candidate oncogene in melanoma and lung carcinomas. SETDB1 methylates lysine 9 of histone 3 (H3K9), utilizing S-adenosylmethionine (SAM) as the methyl donor. Its activity has been shown to be regulated by its partner protein ATF7IP, and here we examine the contribution of ATF7IP to the in vitro activity and substrate specificity of SETDB1. SETDB1 and ATF7IP were co-expressed in Sf21 insect cells and 1:1 stoichiometric complexes were purified for comparison against apo-SETDB1 enzyme. We employed both radiometric flashplate-based and MALDI mass spectrometry assays to follow methylation on histone 3 15-mer peptides where lysine 9 was either unmodified, mono-, or di-methylated. These two methods provide orthogonal readouts on H3K9 methylation: the radiometric flashplate-based assay employs 3H-labeled SAM and provides quantitation of labeled, methylated peptide. The MALDI mass spectrometry assay offers quantitation of consumption and accumulation of each individual H3K9 methylation state (H3K9me0, me1, me2, and me3). Results show that both apo-SETDB1 and SETDB1:ATF7IP complex catalyzed both monomethylation and dimethylation of H3K9 peptide substrates, but were unable to perform H3K9 trimethylation. While ATF7IP did not impact the substrate methylation profile, the activity of the complex was lower than apo-SETDB1 by a factor of 4. This difference was due to a decrease in the value of kcat as the substrate KM values were comparable between apo-SETDB1 and the SETDB1:ATF7IP complex. ATF7IP therefore does not alter SETDB1's substrate specificity. H3K9 monomethylation and dimethylation by SETDB1 occurred in a distributive manner and was unaffected by the presence of ATF7IP. This finding is important as H3K9 can be methylated by HMTs other than SETDB1 and a distributive mechanism would allow for interplay between multiple HMTs on H3K9me1 and H3K9me2. The results presented here indicate that ATF7IP does not alter substrate specificity of SETDB1 in vitro, and though it decreases SETDB1's catalytic activity, our data points to a nonenzymatic function of ATF7IP in regulating SETDB1 function. Citation Format: Aravind Basavapathruni, Jodi Gureasko, Margaret Porter Scott, P. Ann Boriack-Sjodin, Timothy J. Wigle, Thomas V. Riera, Robert A. Copeland. ATF7IP does not alter the substrate specificity of the lysine methyltransferase SETDB1. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 104. doi:10.1158/1538-7445.AM2015-104
Susceptibility of Rat-Derived Cells to Replication by Human Immunodeficiency Virus Type 1 (2001)

Progress in developing a small animal model of human immunodeficiency virus type 1 (HIV-1) disease would greatly facilitate studies of transmission, pathogenesis, host immune responses, and antiviral strategies. In this study, we have explored the potential of rats as a susceptible host. In a single replication cycle, rat cell lines Rat2 and Nb2 produced infectious virus at levels 10- to 60-fold lower than those produced by human cells. Rat-derived cells supported substantial levels of early HIV-1 gene expression, which was further enhanced by overexpression of human cyclin T1. Rat cells displayed quantitative, qualitative, and cell-type-specific limitations in the late phase of the HIV-1 replication cycle including relative expression levels of HIV-1 Gag proteins, intracellular Gag processing, and viral egress. Nb2 cells were rendered permissive to HIV-1 R5 viruses by coexpression of human CD4 and CCR5, indicating that the major restriction on HIV-1 replication was at the level of cellular entry. We also found that primary rat lymphocytes, macrophages, and microglia expressed considerable levels of early HIV-1 gene products following infection with pseudotyped HIV-1. Importantly, primary rat macrophages and microglia, but not lymphocytes, also expressed substantial levels of HIV-1 p24 CA and produced infectious virions. Collectively, these results identify the rat as a promising candidate for a transgenic small animal model of HIV-1 infection and highlight pertinent cell-type-specific restrictions that are features of this species.
Altered trichothecene biosynthesis in TRI6 ‐silenced transformants of Fusarium culmorum influences the severity of crown and foot rot on durum wheat seedlings (2011)

An RNA silencing construct was used to alter mycotoxin production in the plant pathogenic fungus Fusarium culmorum, the incitant of crown and foot rot on wheat. The transformation of a wild-type strain and its nitrate reductase-deficient mutant with inverted repeat transgenes (IRTs) containing sequences corresponding to the trichothecene regulatory gene TRI6 was achieved using hygromycin B resistance as a selectable marker. Southern analysis revealed a variety of integration patterns of the TRI6 IRT. One transformant underwent homologous recombination with deletion of the endogenous TRI6 gene, whereas, in another transformant, the TRI6 IRT was not integrated into the genome. The TRI6 IRT did not alter the physiological characteristics, such as spore production, pigmentation or growth rate, on solid media. In most transformants, a high TRI6 amplification signal was detected by quantitative reverse transcription-polymerase chain reaction, corresponding to a TRI6-hybridizing smear of degraded fragments by Northern analysis, whereas TRI5 expression decreased compared with the respective nontransformed strain. Four transformants showed increased TRI5 expression, which was correlated with a dramatic (up to 28-fold) augmentation of deoxynivalenol production. Pathogenicity assays on durum wheat seedlings confirmed that impairment of deoxynivalenol production in the TRI6 IRT transformants correlated with a loss of virulence, with decreased disease indices ranging from 40% to 80% in nine silenced strains, whereas the overproducing transformants displayed higher virulence compared with the wild-type.
Figure S7 from Impaired Death Receptor Signaling in Leukemia Causes Antigen-Independent Resistance by Inducing CAR T-cell Dysfunction (2023)

&lt;p&gt;Death receptor gene expression is associated with clinical outcomes.&lt;/p&gt;
The cycle of genome-directed medicine (2009)

The genome era in medicine is upon us. Questions that arise from patient and family care are a watershed for research and technology, which in turn fuel the cycle of opportunity for impact through delivery of health services, which feeds back to families. Medical infrastructure needs to adapt to the dramatic pace of technology development in the wake of the Human Genome Project, in order for genome data to be delivered as information and applied as knowledge to benefit health.
The improvement of ride quality of tractor using semi-active suspension (2002)

Recently, the development stage of agricultural vehicles such as the tractor has been focused on new demands to improve the cabin environment. Especially, the ride quality of the tractor is been looking for as an important factor. For this purpose, rubber bushes have installed between the cabin and the frame of the tractor to reduce road vibration to the cabin. However, this device doesn't sufficiently suppress vibration and there's a need to improve from a driver's standpoint. This paper shows the method to improve the ride quality by reducing the road vibration installing a semi-active suspension at the rear cabin mount position of the tractor. We investigated the effect of decrease in vibration of semi-active suspension applied the sky-hook control by an experiment and simulation method at one degree of freedom model, and driving test. The result shows that the semi-active suspension applied the sky-hook control will be able to realize the decrease in vibration.
Detection of Contaminating Enzymatic Activity in Plant-Derived Recombinant Biotechnology Products (2014)

Residual impurities in recombinantly produced protein biologics, such as host cell proteins (HCP), can potentially cause unwanted toxic or immunogenic responses in patients. Additionally, undetected impurities found in recombinant proteins used in cell culture may adversely impact basic research and biotechnology applications. Currently, the enzyme-linked immunosorbent assay (ELISA) is the standard for detection of residual HCP contamination in recombinantly produced biologics. Alternatively, two-dimensional liquid chromatography coupled to mass spectrometry is being developed as a tool for assessing this critical quality attribute. Both of these methods rely on the direct detection of HCPs and some previous knowledge of the contaminant. For contaminating enzymes, the mass level of the impurity may fall below the threshold of detection of these methods and underestimate the true impact. To address this point, here we demonstrate facile detection and characterization of contaminating phytase activity in rice-derived recombinant human serum albumin (rHSA) using a sensitive, label-free nuclear magnetic resonance (NMR) spectroscopy assay. We observed varying degrees of phytase contamination in biotechnology-grade rHSA from various manufacturers by monitoring the degradation of adenosine-5'-triphosphate and myo-inositol-1,2,3,4,5,6-hexakisphosphate by (31)P NMR. The observed lot-to-lot variability may result in irreproducible cell culture results and should be evaluated as a possible critical quality attribute in plant-derived biotherapeutics.
Two-wavelength holographic recording in photopolymer using four-energy-level system: experiments and modeling (2014)

We investigate a two-wavelength method for recording a persistent hologram in a doped photopolymer. The recording method is based on two separated optical excitations of the four-energy-level system of the doped element, one at 325 nm as the sensitizing wavelength and the other at 647 nm as the writing wavelength, allowing for an experimental demonstration of nondestructive readout in phenanthrenequinonedoped poly(methyl methacrylate). Further, a four-energy-level rate equations model is proposed for describing the dynamics of hologram recording. The model successfully explains our experimental finding and further provides a general method to investigate such a two-wavelength holographic recording in photopolymer.
Relationship between Side Chain Structure and 14-Helix Stability of β3-Peptides in Water (2004)

Folded polymers are used in Nature for virtually every vital process. Nonnatural folded polymers, or foldamers, have the potential for similar versatility, and the design and refinement of such molecules is of considerable current interest. Here we report a complete and systematic analysis of the relationship between side chain structure and the 14-helicity of a well-studied class of foldamers, beta(3)-peptides, in water. Our experimental results (1) verify the importance of macrodipole stabilization for maintaining 14-helix structure, (2) provide comprehensive evidence that beta(3)-amino acids branched at the first side chain carbon are 14-helix-stabilizing, (3) suggest a novel role for side chain hydrogen bonding as an additional stabilizing force in beta(3)-peptides containing beta(3)-homoserine or beta(3)-homothreonine, and (4) demonstrate that diverse functionality can be incorporated into a stable 14-helix. Gas- and solution-phase calculations and Monte Carlo simulations recapitulate the experimental trends only in the context of oligomers, yielding insight into the mechanisms behind 14-helix folding. The 14-helix propensities of beta(3)-amino acids differ starkly from the alpha-helix propensities of analogous alpha-amino acids. This contrast informs current models for alpha-helix folding, and suggests that 14-helix folding is governed by different biophysical forces than is alpha-helix folding. The ability to modulate 14-helix structure through side chain choice will assist rational design of 14-helical beta-peptide ligands for macromolecular targets.
Amorphous iridium complexes for electrophosphorescent light emitting devicesElectronic supplementary information (ESI) available: experimental details, synthetic and spectroscopic data. See http://www.rsc.org/suppdata/cc/b2/b200957a/ (2002)

Iridium complexes with fluorene-modified phenylpyridine ligands are resistant to crystallization and can be used in the fabrication of single layer light emitting diodes.
Assessment of the morphological and functional effects of the anti‐angiogenic agent SU11657 on 9L gliosarcoma vasculature using dynamic susceptibility contrast MRI (2007)

To investigate the influence of anti-angiogenic agents on tumor perfusion, we employed a dynamic susceptibility contrast (DSC)-MRI method that utilizes a simultaneous gradient-echo (GE) and spin-echo (SE) imaging sequence to derive perfusion parameters (blood flow, blood volume, and mean transit time (MTT)). These parameters are sensitive to both the total vasculature (from the GE data) and the microvasculature (from the SE data), and can also provide a measure of the mean vessel diameter (mVD). This approach was used to evaluate the response of a 9L rat brain tumor model to 20 mg/kg and 40 mg/kg of the anti-angiogenic agent SU11657. The 20-mg/kg dose significantly decreased mVD by 29.9% (P = 0.02). The 40-mg/kg dose significantly decreased mVD by 30.4% (P = 0.0007), SE blood volume by 31.8% (P = 0.03), GE and SE MTT by 46.9% (P = 0.03) and 62.0% (P = 0.0005), and increased GE and SE blood flow by 36.6% (P = 0.04) and 52.6% (P = 0.02). These findings demonstrate that DSC-MRI perfusion methods can play a key role in the noninvasive evaluation of morphological and functional changes in tumor vasculature in response to therapy.
Characteristics of Self-Deployment in Origami-Based Systems (2019)

Abstract The potential of compliant mechanisms and related origami-based mechanical systems to store strain energy make them ideal candidates for applications requiring an actuation or deployment process, such as space system arrays, minimally invasive surgical devices and deployable barriers. Many origami structures can be thought of as a compliant mechanism because, like compliant mechanisms, its function is performed through the elastic deformation of its members. This stored strain energy could prove useful. There are opportunities using strain energy to develop approaches to deploy particular mechanical systems. In order to better understand the principles of self-actuation and promote the designs of such systems, a taxonomy of deployable origami mechanisms is presented. This taxonomy demonstrates that there are several different types of deployable origami mechanisms and provides an organizational method to better understand the design space. Characteristics of self deployment in concentrated, deployable origami strain energy mechanisms with internal actuation are identified and examples of strain energy based deployment are provided.