gebhart/patrepeval huggingface dataset:
Supplementary Figure 1 from PPP2R2C Loss Promotes Castration-Resistance and Is Associated with Increased Prostate Cancer-Specific Mortality (2023)

&lt;p&gt;PDF, 133K, PPP2R2C knockdown does not significantly increase proliferation of prostate cancer cell lines grown in androgen-replete conditions.&lt;/p&gt;
On the Cooperative Exploitation of Antenna Sensitivity and Auto-Tuning Capability of UHF RFID Chip. Application to Temperature Sensing (2018)

In this paper, a UHF RFID temperature sensing tag is introduced. The sensing operation combines the antenna sensitivity and an RFID chip with the capability of auto-tuning. The temperature variations have an impact on the substrate properties allowing the temperature to be transduced into a mismatch between the antenna and the RFID chip. This work proposes an original approach which relies on the usage of an RFID chip which offers an auto-tune capability, in this case the Impinj-Monza R6. The principle is to exploit this option in order to evaluate the temperature value sensed by the tag in the RFID reader's end. The proposed RFID sensor tag is completely passive, exceeds a read range of 9 meters and has a small size 57×15×1.27 (mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sup> ).
“High-load” polyethylene glycol-polystyrene (PEG-PS) graft supports for solid-phase synthesis (1998)

The choice of a polymeric support is a key factor for the success of solid-phase methods for syntheses of organic compounds and biomolecules such as peptides and oligonucleotides. Classical Merrifield solid-phase peptide synthesis (SPPS), performed on low cross-linked hydrophobic polystyrene (PS) beads, sometimes suffers from sequence-dependent coupling difficulties. The concept of incorporating polyethylene glycol (PEG) into supports for solid-phase synthesis represents a successful approach to alleviating such problems. Previous reports from our laboratories have shown the advantages of "low-load" PEG-PS (0.15-0.25 mmol/g) for SPPS. Herein, we demonstrate that the beneficial aspects of the PEG-PS concept can be extended with resins that have higher loadings (0.3-0.5 mmol/g).
Recognition of human and animal movement using infrared video streams (2005)

Distinguishing human motion from animal motion is important in many applications using infrared video streams, such as surveillance systems for homeland security and collision avoidance systems for nighttime driving safety. In this paper we present a technique to distinguish human motion from animal motion using infrared video sequences. In our technique, we uses frame differencing to represent object motion. Space-time correlation is used to characterize different type of motions. Our motion features are defined by Renyi entropy and mean values calculated from the correlations. A support vector machine-based classifier is used to classify the motion features. Our experimental results show that our technique is quite effective at distinguishing human motion from animal motion using infrared video sequences.
Ultrasound Thermometry for HIFU-Therapy (2021)

Abstract High-Intensity Focused Ultrasound (HIFU) is an alternative tumour therapy with the ability for non-invasive thermal ablation of tissue. For a safe application, the heat deposition needs to be monitored over time, which is currently done with Magnetic Resonance Imaging. Ultrasound (US) based monitoring is a promising alternative, as it is less expensive and allows the use of a single device for both therapy and monitoring. In this work, a method for spatial and temporal US thermometry has been investigated based on simulation studies and in-vitro measurements. The chosen approach is based on the approximately linear dependence between temperature and speed of sound (SoS) in tissue for a given temperature range. By tracking the speckles of successive B-images, the possibility of detecting local changes in SoS and therefore in temperature is given. A speckle tracking algorithm was implemented for 2D and 3D US thermometry using a spatial compounding method to reduce artifacts. The algorithm was experimentally validated in an agar-based phantom and in porcine tissue for temperature rises up to △ 8°C. We used a focusing single element US transducer as therapeutic probe, a linear (/matrix array) transducer with 128 (/32∙32) elements for imaging and thermocouples for validation and calibration. In all experiments, both computational and in-vitro, we succeeded in monitoring the thermal induced SoS changes over time. The in-vitro measurements were in good agreement with the simulation results and the thermocouple measurements (rms temperature difference = 0.53 °C, rms correlation coefficient = 0. 96).
Modeling and identification of metabolic systems (1981)

Introductory principles of physiological systems analysis by computer simulation or computation are introduced. The problems of model formulation, identification, and validation are examined. Selected guidelines for successful modeling are suggested, and examples of such applications in the field of endocrinology and metabolism are given.
Synaptic polarity of the interneuron circuit controlling C. elegans locomotion (2013)

Caenorhabditis elegans is the only animal for which a detailed neural connectivity diagram has been constructed. However, synaptic polarities in this diagram, and thus, circuit functions are largely unknown. Here, we deciphered the likely polarities of seven pre-motor neurons implicated in the control of worm's locomotion, using a combination of experimental and computational tools. We performed single and multiple laser ablations in the locomotor interneuron circuit and recorded times the worms spent in forward and backward locomotion. We constructed a theoretical model of the locomotor circuit and searched its all possible synaptic polarity combinations and sensory input patterns in order to find the best match to the timing data. The optimal solution is when either all or most of the interneurons are inhibitory and forward interneurons receive the strongest input, which suggests that inhibition governs the dynamics of the locomotor interneuron circuit. From the five pre-motor interneurons, only AVB and AVD are equally likely to be excitatory, i.e., they have probably similar number of inhibitory and excitatory connections to distant targets. The method used here has a general character and thus can be also applied to other neural systems consisting of small functional networks.
Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles (2018)

Enrich And Expand Rare Antigen-specific T Cells With Magnetic Nanoparticles - Video. JoVE publishes peer-reviewed scientific video protocols to accelerate biological, medical, chemical and physical research. Watch our scientific video articles. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research, scientific journals, and education. Millions of scientists, educators and students at thousands of universities, colleges, hospitals and biopharmaceutical companies worldwide use JoVE for their research, teaching and learning.
Full-wave spectral-domain analysis of compensation of microstrip discontinuities using triangular subdomain functions (1992)

A full-wave spectral-domain analysis of compensation for a variety of microstrip discontinuities, including open-ends, bounds and T-junctions, is presented. To properly model the discontinuities with miters as well as 90 degrees corners, vector-valued triangular subdomain functions are used as both expansion and testing functions in the moment method procedure. Special consideration is given to the numerical treatment of the reaction integral between two triangular subdomains such that rather complicated geometrical configurations can be handled very efficiently. Comparison of some numerical results with available experimental data shows excellent agreement. The losses due to radiation and surface waves for some discontinuities are also included.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Is CYBERNOSE®an instrument for measuring odor space? (2014)

All human senses, other than smell, can be readily represented by definable physical surrogates. Our sense of hot and cold approximates temperature as measured by a thermometer; touch equates to pressure; balance measures orientation and acceleration; vision integrates measures of the wavelengths and intensities of a portion of the electromagnetic spectrum with the four dimensions of space and time; hearing fundamentally equates to the amplitude and frequency of vibrations. Taste, apart from our bitter sense, measures the concentrations of acids, salts, a few amino acids and a number of sugars. Our sense of smell is different. There is no instrument that can measure the fundamental properties that our noses respond to indeed we don’t really know what those properties are. Whilst there is some loose relation between sensory perception of smells and chemical structures, this only seems to apply to closely connected chemical species and breaks down once we move beyond that. In principle we could objectively define an odor space, to sit alongside the human or animal percept, that could be measured with a set of odorant receptors, precisely definable as strings of amino acids expressed under standard conditions. Such odorant receptors would define the dimensions of odorant space. Of course, currently, there are many practical obstacles to employing biological odorant receptors for this purpose. One of the difficulties is the existence of many millions of different odorant receptor sequences belonging to at least four molecular families and distributed among millions of different metazoan species. We could define a human receptor-based odor space or a dog or a fly or nematode worm receptor-based odor space. They would differ, in some respects profoundly. Nevertheless, solving the aforementioned practical problems would not only support many practical applications but also allow us to reproducibly define the dimensions of odor space and make “smell” an objective measurable property. Our approach to this is through machine olfaction. Currently we are working with off-the shelf electronic noses as well as developing our own bioelectronic nose, the CYBERNOSE instrument. In the former case, we looked to biology, specifically to published data on Drosophila olfaction, to guide our understanding of how solid state sensors encode odor space [1]. The take home message appears to be that the inadequacies of current solid state sensors prevent them being very useful for characterising and defining odor space, as proposed by Shurmer & Whitehouse (1993). On the other hand, we believe that development of the CYBERNOSE instrument, which incorporates odorant biosensors, offers a viable approach to characterise defined, standardised odor spaces, based on but not limited to what is available to the nematode worm Caenorhabditis elegans.
The Potential for Divalent Rare Earth Spectroscopy (Beyond Eu2+) in S2MgSi2O7? (2006)

Abstract not Available.
A broadband stacked patch antenna with enhanced antenna gain by an optimized ellipsoidal reflector for X-band applications (2011)

A broadband stacked patch antenna, based on an aperture coupled feeding with two patch elements is presented. The transmission line model is introduced as one possibility for the design of an aperture coupled antenna. 3D electromagnetic field simulations are used for optimization and allow a detailed investigation of the influence of the different antenna parameters on the return loss and the antenna gain. To minimize the effect of an undesired formed opposite side lobe of the antenna, resulting in a poor front-to-back ratio, an optimized ellipsoidal reflector is presented to focus the radiation in one main direction. A good return loss in a bandwidth of more than 4 GHz, i.e. 42%, with a maximum antenna gain of 18.1 dBi is achieved. A first prototype verifies the simulation results and the functionality of the developed antenna design.
Cyclobut-A and cyclobut-G: broad-spectrum antiviral agents with potential utility for the therapy of AIDS (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCyclobut-A and cyclobut-G: broad-spectrum antiviral agents with potential utility for the therapy of AIDSDaniel W. Norbeck, Earl Kern, Seiji Hayashi, William Rosenbrook, Hing Sham, Thomas Herrin, Jacob J. Plattner, John Erickson, Jacob Clement, and Cite this: J. Med. Chem. 1990, 33, 5, 1281–1285Publication Date (Print):May 1, 1990Publication History Published online1 May 2002Published inissue 1 May 1990https://pubs.acs.org/doi/10.1021/jm00167a002https://doi.org/10.1021/jm00167a002research-articleACS PublicationsRequest reuse permissionsArticle Views286Altmetric-Citations114LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
TAMI-79. THERAPEUTIC REVERSAL OF PRENATAL PONTINE ID1 SIGNALING IN DIPG (2021)

Abstract Diffuse intrinsic pontine glioma (DIPG) is a highly aggressive pediatric brain tumor with rare survival beyond two years. This poor prognosis is largely due to the tumor's highly infiltrative and invasive nature. Nearly 80% of DMGs harbor K27M mutation in the genes encoding histone H3.1 (H3F3A) or H3.3 (HISTIH3B), often with concurrent ACVR1 mutation. Inhibitor of DNA-binding (ID) proteins are key transcriptional regulators of genes involved in lineage commitment and are associated with invasiveness and poor clinical outcomes in multiple human cancers. Introduction of H3K27M and ACVR1 mutations increase ID1 expression in cultured astrocytes, but this has not been confirmed in human tumors or targeted therapeutically. We developed an in-utero electroporation (IUE) murine H3K27M-driven tumor model, which demonstrates increased ID1 expression in H3K27M- and ACVR1-mutated tumor cells. Exome and transcriptome sequencing analysis of multi-focal DMG tumors (n=52) and normal brain tissue revealed that increased ID1 expression is associated with H3K27M/ACVR1-mutation and brainstem location, and correlates with poor survival in patients. ChIP-sequencing for H3K27ac and H3K27me3 in multiple DMG tumors (n=5) revealed that the ID1 gene is epigenetically active, which matches the epigenetic state of murine prenatal hindbrain cells. Higher ID1-expressing astrocyte-like DIPG cells share a similar transcriptional program with ID1+/SPARCL1+ positive oligo/astrocyte-precursor (OAPC) cells from the developing human brain and demonstrate upregulation of gene sets involved in regulation of cell migration. Both genetic and pharmacologic [cannabidiol (CBD)] suppression of ID1 result in decreased DIPG cell invasion/migration in vitro and invasion/tumor growth in multiple in vivo models. Mechanistically, CBD reduces proliferation through production of reactive oxygen species. Further, DIPG patients treated off-trial with CBD (n=15) displayed reduced ID1 tumor expression and improved overall survival. In summary, ID1 is upregulated in DIPG through K27M-mediated epigenetic reactivation of a developmental OAPC-like transcriptional state, and ID1-driven invasiveness of DIPG is therapeutically targetable with CBD.
Intravesical Liposome and Antisense Treatment for Detrusor Overactivity and Interstitial Cystitis/Painful Bladder Syndrome (2014)

Purpose. The following review focuses on the recent advancements in intravesical drug delivery, which brings added benefit to the therapy of detrusor overactivity and interstitial cystitis/painful bladder syndrome (IC/PBS). Results. Intravesical route is a preferred route of administration for restricting the action of extremely potent drugs like DMSO for patients of interstitial cystitis/painful bladder syndrome (IC/PBS) and botulinum toxin for detrusor overactivity. Patients who are either refractory to oral treatment or need to mitigate the adverse effects encountered with conventional routes of administration also chose this route. Its usefulness in some cases can be limited by vehicle (carrier) toxicity or short duration of action. Efforts have been underway to overcome these limitations by developing liposome platform for intravesical delivery of biotechnological products including antisense oligonucleotides. Conclusions. Adoption of forward-thinking approaches can achieve advancements in drug delivery systems targeted to future improvement in pharmacotherapy of bladder diseases. Latest developments in the field of nanotechnology can bring this mode of therapy from second line of treatment for refractory cases to the forefront of disease management.
Adaptive fault-tolerant routing in hypercube multicomputers (1990)

A connected hypercube with faulty links and/or nodes is called an injured hypercube. A distributed adaptive fault-tolerant routing scheme is proposed for an injured hypercube in which each node is required to know only the condition of its own links. Despite its simplicity, this scheme is shown to be capable of routing messages successfully in an injured n-dimensional hypercube as long as the number of faulty components is less than n. Moreover, it is proved that this scheme routes messages via shortest paths with a rather high probability, and the expected length of a resulting path is very close so that of a shortest path. Since the assumption that the number of faulty components is less than n in an n-dimensional hypercube might limit the usefulness of the above scheme, a routing scheme based on depth-first search which works in the presence of an arbitrary number of faulty components is introduced. Due to the insufficient information on faulty components, however, the paths chosen by this scheme may not always be the shortest. To guarantee all messages to be routed via shortest paths, the authors propose to equip every node with more information than that on its own links. The effects of this additional information on routing efficiency are analyzed, and the additional information to be kept at each node for the shortest path routing is determined. Several examples and remarks are given to illustrate the results.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Cholinergic Nerve Differentiation of Mesenchymal Stem Cells Derived from Long-Term Cryopreserved Human Dental Pulp In Vitro and Analysis of Their Motor Nerve Regeneration Potential In Vivo (2018)

The reduction of choline acetyltransferase, caused by the loss of cholinergic neurons, leads to the absence of acetylcholine (Ach), which is related to motor nerve degeneration. The aims of the present study were to evaluate the in vitro cholinergic nerve differentiation potential of mesenchymal stem cells from cryopreserved human dental pulp (hDPSCs-cryo) and to analyze the scale of in vivo motor nerve regeneration. The hDPSCs-cryo were isolated and cultured from cryopreserved dental pulp tissues, and thereafter differentiated into cholinergic neurons using tricyclodecane-9-yl-xanthogenate (D609). Differentiated cholinergic neurons (DF-chN) were transplanted into rats to address sciatic nerve defects, and the scale of in vivo motor nerve regeneration was analyzed. During in vitro differentiation, the cells showed neuron-like morphological changes including axonal fibers and neuron body development, and revealed high expression of cholinergic neuron-specific markers at both the messenger RNA (mRNA) and protein levels. Importantly, DF-chN showed significant Ach secretion ability. At eight weeks after DF-chN transplantation in rats with sciatic nerve defects, notably increased behavioral activities were detected with an open-field test, with enhanced low-affinity nerve growth factor receptor (p75NGFR) expression detected using immunohistochemistry. These results demonstrate that stem cells from cryopreserved dental pulp can successfully differentiate into cholinergic neurons in vitro and enhance motor nerve regeneration when transplanted in vivo. Additionally, this study suggests that long-term preservation of dental pulp tissue is worthwhile for use as an autologous cell resource in the field of nerve regeneration, including cholinergic nerves.
Effect of sunlight on the infectivity ofCryptosporidium parvumin seawater (2007)

The prevalence of pathogenic microorganisms in seawater can result in waterborne and food borne outbreaks. This study was performed to determine the effect of sunlight and salinity on the die-off of Cryptosporidium parvum . Cryptosporidium parvum oocysts, Escherichia coli , and MS2 coliphage were seeded into tap water and seawater samples and then exposed to sunlight. The die-off of C. parvum in seawater, as measured by infectivity, was greater under sunlight (–3.08 log 10 ) than under dark conditions (–1.31 log 10 ). While, no significant difference was recorded in the die-off of C. parvum, under dark conditions, in tap water as compared to seawater (P &lt; 0.05), indicating that the synergistic effect of salinity and sunlight was responsible for the enhanced die-off in seawater. The die-off of MS2 coliphage and E. coli was greater than that observed for C. parvum under all tested conditions. This indicates that these microorganisms cannot serve as indicators for the presence of C. parvum oocysts in seawaters. The results of the study suggest that C. parvum can persist as infectious oocysts for a long time in seawater and can thus pose a serious hazard by direct and indirect contact with humans.