gebhart/patrepeval huggingface dataset:
Down syndrome-associated haematopoiesis abnormalities created by chromosome transfer and genome editing technologies (2014)

Infants with Down syndrome (DS) are at a high risk of developing transient abnormal myelopoiesis (TAM). A GATA1 mutation leading to the production of N-terminally truncated GATA1 (GATA1s) in early megakaryocyte/erythroid progenitors is linked to the onset of TAM and cooperated with the effect of trisomy 21 (Ts21). To gain insights into the underlying mechanisms of the progression to TAM in DS patients, we generated human pluripotent stem cells harbouring Ts21 and/or GATA1s by combining microcell-mediated chromosome transfer and genome editing technologies. In vitro haematopoietic differentiation assays showed that the GATA1s mutation blocked erythropoiesis irrespective of an extra chromosome 21, while Ts21 and the GATA1s mutation independently perturbed megakaryopoiesis and the combination of Ts21 and the GATA1s mutation synergistically contributed to an aberrant accumulation of skewed megakaryocytes. Thus, the DS model cells generated by these two technologies are useful in assessing how GATA1s mutation is involved in the onset of TAM in patients with DS.
New enzyme linked immunosorbent assay using recombinant antigens from Saccharopolyspora rectivirgula for farmer's lung disease serodiagnosis (2012)

Saccharopolyspora rectivirgula (SR) is the main etiologic agent of the Farmer9s Lung Disease (FLD). Serodiagnosis using immunoprecipitation techniques with crude antigens has proved effective but lacks of standardization. We aim at producing specific recombinant antigens (rAg) to develop a new standardized enzyme-linked immunosorbent assay (ELISA). A database of putative proteins was first specifically created after partially sequencing of SR Subsequently, proteins were analyzed by two-dimensional electrophoresis and revealed by Western-blot analysis with serum from 9 patients with FLD in comparison with the serum of 4 exposed controls. Among the 77 visible spots, 42 were analyzed by mass spectrometry and 28 proteins were identified. Sixteen FLD-specific proteins were produced as rAg in Escherichia coli . ELISA with 16 rAgs were performed using serums of 20 FLD patients from France and Switzerland and 27 controls. Results were analyzed by receiver operating characteristics curve. Five showed an area under the curve (AUC) above 0.75 and a sensitivity equal or greater than 75%. (named SR1FA, SR9, SR13, SR17 and SR25). The protein performing the best (SR9) reached 80% sensitivity and 85% specificity with an AUC of 0.90. ELISA using rAgs specific from SR were effective for serodiagnosis of FLD. The use of antigen panels including rAg specific from other micro-organisms involved in FLD ( Aspergillus, Lichtheimia, Wallemia ) may increase the diagnosis performance of the ELISA. A prospective study including FLD patients from other countries (Finland, Canada), exposed to other strains of SR , should be done for a large scale validation.
Myc Represses miR15a/Mir-16-1 Expression through Recruitment of HDAC3 In Mantle Cell Lymphoma (2010)

Abstract Abstract 3121 MicroRNAs (miR)-15a and miR-16-1 are known to act as tumor suppressors. Expression of these miRNAs inhibits cell proliferation, promotes apoptosis of cancer cells, and suppresses tumorigenicity by targeting multiple oncogenes. Loss or down-regulation of these miRNAs has been reported in a variety of hematopoietic and solid tumors. Our recent study demonstrated that miR-15a/16-1 are down-regulated in the majority of patients with mantle cell lymphoma (MCL, Zhao et al., Blood. 2010 1;115(13):2630-9). However, the mechanism responsible for miR-15a/16 suppression was unknown. Here, we have investigated mechanism of miR-15a/16-1 repression and its transcriptional and epigenetic regulation by Myc and histone deacetylase (HDAC) in MCL. Over-expression of Myc protein was detected in all MCL cell lines, and miR-15a/16 and DLEU-2 mRNA were significantly up-regulated in Mino and Jeko-1 cells when Myc was depleted by c-Myc siRNA. Treatment with pan HDAC inhibitor, SAHA and specific HDAC-3 shRNA resulted in increase of miR15a/16 expression. Co-immunoprecipitation study showed that c-Myc interacted with HDAC3. Moreover, chromatin immunoprecipitation (ChIP)demonstrated that both Myc and HDAC3 co-localized to the two promoters of miR-15a/16 cluster gene, DLEU-2. Luciferase reporter assay confirmed that both c-Myc and HDAC3 inhibited transcriptional activity of two E-box (Myc-binding)-specific DLEU-2 promoters. Computational analysis and mutagenesis study further supported direct association of Myc with miR-15a/16 promoter resulting in HDAC3-mediated transcriptional repression. These findings highlight an important role for HDAC-3 in Myc-driven lymphoma cell proliferation and reveal novel transcriptional and posttranscriptional mechanisms in Myc-mediated malignant transformation in MCL. Disclosures: No relevant conflicts of interest to declare.
IRRIGATION WATER UTILIZATION AS A FUNCTION OF SOIL MECHANICS AND ITS PROPERTIES (2011)

An experiment was conducted for identifying the most efficient tillage method using the sub-soiling plough for enhancing some of physical properties of the heavy clayey soils. The experiment was conducted on a heavy clayey soil of an area 1800 m² located in Serewa village – Belqas – Daqahlia. This area divided into 4 adjacent plots, each one has a dimensions of (9m x50m) and was allocated only, for one treatment. The surface irrigation was the system applied and the amount of irrigation water added was equal for each plot (22.5m³) and was applied at the same time for all plots. Furthermore, an accounting for the rate of water flux in mm/day beneath soil profile along subsequent ten days from saturation was the last objective of this research. Only, four treatments were involved in this research as (Treat.1): sub-soiling plough at 40cm depth with a lateral spacing of 150cm, (T2): sub-soiling plough at 60cm depth with a lateral spacing of 150cm, (T3): sub-soiling plough at 40cm depth with a lateral spacing of 300cm and (T4): sub-soiling plough at 60cm with a lateral spacing of 300cm. For all plots, the sub-soiling plough was followed by chisel plough at 20cm depth and laser-controlled leveller at the dead level. All the data obtained clearly indicated that, the second treatment (T2) achieved the highest mean value of soil infiltration rate (2.2 mm/h) over all treatments and the lowest mean values for the cone penetration resistance, water content and bulk density at the surface layer of soil profile after tillage (2.41 M.Pa), (17.74 % w/w) and (1.17 g/cm³), respectively. Whereas, at saturation, it achieved the highest mean value of the water content (62.78 %w/w) and the lowest mean value of penetration resistance (.01 M.Pa). On the other hand, the mean value of the water content rested in the soil of the second treatment at the tenth day from saturation was (24.68 %w/w), producing the highest water flux (4.29 mm/day). Also, the second treatment achieved the shortest period of time needed until all amount of the deluge imposed on the soil surface after irrigation be completely infiltrated (20 h.).
Tunicamycin inhibits ability of irradiated L. braziliensis promastigotes to protect against infection with virulent organisms in vivo (1986)

Intravenous immunization with irradiated promastigotes of L. braziliensis can protect BALB/c mice from the progressive, non-healing lesions caused by intradermal inoculation of virulent L. braziliensis. If mice are previously challenged with irradiated parasites via a subcutaneous route before intravenous innoculation no such protection is observed. They have examined the effect of inhibition of N-glycosylation of glycopeptides on the ability of irradiated promastigotes to produce these effects. At 1..mu..g/ml tunicamycin caused minimal (< 10%) suppression of /sup 3/H-leucine incorporation into parasite membranes, yet a 40%-60% inhibition of incorporation of /sup 3/H-mannose. Swainsonine, as expected, at 1-2..mu..M affected neither metabolic labelling procedure. Tunicamycin-treated promastigotes were unable to immunize mice for protection, but were apparently as effective as untreated organisms in blocking protection (by intravenous immunization) when given subcutaneously. These data are consistent with the notion that the protection inducing determinants are dependent upon N-glycosylation for their expression, unlike the determinants responsible for suppression of protection.
Airway Epithelium: A Neglected but Crucial Cell Type in Asthma Pathobiology (2023)

The features of allergic asthma are believed to be mediated mostly through the Th2 immune response. In this Th2-dominant concept, the airway epithelium is presented as the helpless victim of Th2 cytokines. However, this Th2-dominant concept is inadequate to fill some of the vital knowledge gaps in asthma pathogenesis, like the poor correlation between airway inflammation and airway remodeling and severe asthma endotypes, including Th2-low asthma, therapy resistance, etc. Since the discovery of type 2 innate lymphoid cells in 2010, asthma researchers started believing in that the airway epithelium played a crucial role, as alarmins, which are the inducers of ILC2, are almost exclusively secreted by the airway epithelium. This underscores the eminence of airway epithelium in asthma pathogenesis. However, the airway epithelium has a bipartite functionality in sustaining healthy lung homeostasis and asthmatic lungs. On the one hand, the airway epithelium maintains lung homeostasis against environmental irritants/pollutants with the aid of its various armamentaria, including its chemosensory apparatus and detoxification system. Alternatively, it induces an ILC2-mediated type 2 immune response through alarmins to amplify the inflammatory response. However, the available evidence indicates that restoring epithelial health may attenuate asthmatic features. Thus, we conjecture that an epithelium-driven concept in asthma pathogenesis could fill most of the gaps in current asthma knowledge, and the incorporation of epithelial-protective agents to enhance the robustness of the epithelial barrier and the combative capacity of the airway epithelium against exogenous irritants/allergens may mitigate asthma incidence and severity, resulting in better asthma control.
Structure/property relationships in ferroelectric thin films for frequency agile microwave electronics (1998)

Abstract Ferroelectric thin films, deposited by pulsed laser deposition (PLD), are currently being used to develop a new class of tunable microwave circuits based on the electric field dependence of the dielectric constant. Single phase, (100) oriented Ba0.5Sr0.5TiO3 (BST) films have been deposited onto (100) LaAlO3, SrTiO3, and MgO substrates. Interdigitated capacitors patterned on top of the ferroelectric film have been used to measure the dielectric constant and dissipation factor of these films as a function of DC bias and temperature at 1 MHz and as a function of DC bias and frequency (1 to 20 GHz) at room temperature. The dielectric properties of the ferroelectric film is sensitive to both the deposition and post processing conditions. Optical imaging of the ferroelectric films using confocal scanning optical microscopy (CSOM) shows reproducible polarization fluctuations over sub-micrometer length scales for BST films deposited onto SrTiO3 which are not observed for films deposited onto MgO. Dielectric loss in the ferroelectric film is reduced through a combination of post deposition processing and donor/acceptor doping of the films. The lowest dielectric loss measured has been tanδ = 0.01 – 0.005.
Integration Of Fault Detection And Indentification Into A Fault Tolerant Automated Highway System (1997)

This work focuses on vehicle fault detection and identification. It also describes a vehicle health management approach based on analytic redundancy. It describes a point design of fault detection filters and parity equations that are developed for the vehicle longitudinal mode. A preliminary design of a range sensor fault monitoring system is outlined as an application of a new decentralized fault detection filter. This system combines dynamic state information already generated by the existing filter designs with inter-vehicle analytic redundancy
Individually Addressable and Spectrally Programmable Artificial Atoms in Silicon Photonics (2022)

Artificial atoms in solids have emerged as leading systems for quantum information processing tasks such as quantum networking, sensing, and computing. A central goal is to develop platforms for precise and scalable control of individually addressable artificial atoms that feature efficient optical interfaces. Color centers in silicon, such as the recently-isolated carbon-related 'G-center', exhibit emission directly into the telecommunications O-band and can leverage the maturity of silicon-on-insulator (SOI) photonics. Here, we demonstrate the generation, individual addressing, and spectral trimming of G-center artificial atoms in a SOI photonic integrated circuit (PIC) platform. Focusing on the neutral charge state emission at 1278nm, we observe waveguide-coupled single photon emission with an exceptionally narrow inhomogeneous distribution with standard deviation of 1.1nm, an excited state lifetime of 8.3$\pm$0.7ns, and no degradation after months of operation. In addition, we introduce a technique for optical trimming of spectral transitions up to 300 pm (55 GHz) and local deactivation of single artificial atoms. This non-volatile "spectral programming" enables the alignment of quantum emitters into 25 GHz telecommunication grid channels. Our demonstration opens the path to quantum information processing based on implantable artificial atoms in very large scale integrated (VLSI) photonics.
Changes in the Lipid Composition of Candida utilis during the Cell Cycle (1979)

Although contents of triacylglycerols, free sterols and esterified sterols did not alter during the 3·5 h cell cycle of Candida utilis NRRL Y-900 growing synchronously under glucose limitation, changes were detected in the phospholipid content. Phospholipid:sterol molar ratios were lowest in organisms sampled after 2·5 h and highest in those analysed after 0·5 h of the cycle.
Networks : the proceedings of the joint International Conference on Wireless LANs and Home Networks (ICWLHN 2002) and Networking (ICN 2002) : Atlanta, USA, 26-29 August 2002 (2002)

Contents: Wireless Local Area Networks Quality of Service Multicast Networks Home Networks Network Security and Virtual Private Networks Power Management and Mobile Agents MPLS Switching and DiffServ Traffic Engineering and Management Optical Networks Last Mile Technologies and Network Switching TCP Adaptation, Congestion Control, and Protocol Design Network Performance Evaluation Mobile IP and Ad Hoc Networks Mobile Multimedia, Bluetooth, and Sensor Networks Networked Software and Applications 3G Wireless and Software Radio.
Initial Processes of Atomic Layer Deposition of Al2O3 on InGaAs: Interface Formation Mechanisms and Impact on Metal-Insulator-Semiconductor Device Performance (2012)

Interface-formation processes in atomic layer deposition (ALD) of Al₂O₃ on InGaAs surfaces were investigated using on-line Auger electron spectroscopy. Al₂O₃ ALD was carried out by repeating a cycle of Al(CH₃)₃ (trimethylaluminum, TMA) adsorption and oxidation by H₂O. The first two ALD cycles increased the Al KLL signal, whereas they did not increase the O KLL signal. Al₂O₃ bulk-film growth started from the third cycle. These observations indicated that the Al₂O₃/InGaAs interface was formed by reduction of the surface oxides with TMA. In order to investigate the effect of surface-oxide reduction on metal-insulator-semiconductor (MIS) properties, capacitors and field-effect transistors (FETs) were fabricated by changing the TMA dosage during the interface formation stage. The frequency dispersion of the capacitance-voltage characteristics was reduced by employing a high TMA dosage. The high TMA dosage, however, induced fixed negative charges at the MIS interface and degraded channel mobility.
51.3: Electrophoretically Controlled Nematic LCD: Bistability and Multistability with Color Generation (2006)

Abstract Here is presented a nematic LCD based on a novel orientational effect in suspension of the nematic liquid crystal (NLC) with solid nanoparticles, controlled by the optically “hidden” electrophoretic effect: electrophoretically controlled nematic (EPCN) [1,2]. Polarity controlled electromigration of the nanoparticles, results in the stabilization of the molecular alignment and provides the bistable/multistable switching in a conventionally designed LC structure. Multistability, provided by the electrophoretically controlled birefringence effect, allows multistable color switching, stabilized at zero field. This makes it easier to introduce into conventional nematic LC technology a polarity‐controlled bistable/multistable switching with color capability and gives to standard LCD fabrication technique a big opportunity for developing low consumption high informative displays, including low cost large scale flexible displays.
Notch1, Notch2, and Epstein-Barr virus–encoded nuclear antigen 2 signaling differentially affects proliferation and survival of Epstein-Barr virus–infected B cells (2009)

The canonical mode of transcriptional activation by both the Epstein-Barr viral protein, Epstein-Barr virus-encoded nuclear antigen 2 (EBNA2), and an activated Notch receptor (Notch-IC) requires their recruitment to RBPJ, suggesting that EBNA2 uses the Notch pathway to achieve B-cell immortalization. To gain further insight into the biologic equivalence between Notch-IC and EBNA2, we performed a genome-wide expression analysis, revealing that Notch-IC and EBNA2 exhibit profound differences in the regulation of target genes. Whereas Notch-IC is more potent in regulating genes associated with differentiation and development, EBNA2 is more potent in inducing viral and cellular genes involved in proliferation, survival, and chemotaxis. Because both EBNA2 and Notch-IC induced the expression of cell cycle-associated genes, we analyzed whether Notch1-IC or Notch2-IC can replace EBNA2 in B-cell immortalization. Although Notch-IC could drive quiescent B cells into the cell cycle, B-cell immortalization was not maintained, partially due to an increased apoptosis rate in Notch-IC-expressing cells. Expression analysis revealed that both EBNA2 and Notch-IC induced the expression of proapoptotic genes, but only in EBNA2-expressing cells were antiapoptotic genes strongly up-regulated. These findings suggest that Notch signaling in B cells and B-cell lymphomas is only compatible with proliferation if pathways leading to antiapototic signals are active.
Compact SiPM based detector module for time-of-flight PET/MR (2009)

We present a compact detector module for gamma detection in the PET part of a simultaneous ToF-PET/MR system. The module covers an area of 3.3 cm times 3.3 cm with 64 SiPM based readout channels. It is composed of a stack of three PCBs of identical size: The SiPMs on the topmost PCB are read out by two full-custom ASICs located on a second PCB located underneath. A third PCB at the bottom of the stack contains a local voltage regulator, an FPGA for ASIC control and data processing, and DACs to generate bias voltages for the readout ASICs and the SiPM devices. An LYSO scintillator block is optically coupled to the SiPMs for gamma to light conversion.
Pathophysiology of medication overuse headache: Insights and hypotheses from preclinical studies (2011)

Introduction: Medication overuse headache (MOH) is a clinical concern in the management of migraine headache. MOH arises from the frequent use of medications used for the treatment of a primary headache. Medications that can cause MOH include opioid analgesics as well as formulations designed for the treatment of migraine, such as triptans, ergot alkaloids, or drug combinations that include caffeine and barbiturates. Literature review: Gathering evidence indicates that migraine patients are more susceptible to development of MOH, and that prolonged use of these medications increases the prognosis for development of chronic migraine, leading to the suggestion that similar underlying mechanisms may drive both migraine headache and MOH. In this review, we examine the link between several mechanisms that have been linked to migraine headache and a potential role in MOH. For example, cortical spreading depression (CSD), associated with migraine development, is increased in frequency with prolonged use of topiramate or paracetamol. Conclusions: Increased CGRP levels in the blood have been linked to migraine and elevated CGRP can be casued by prolonged sumatriptan exposure. Possible mechanisms that may be common to both migraine and MOH include increased endogenous facilitation of pain and/or diminished diminished endogenous pain inhibition. Neuroanatomical pathways mediating these effects are examined.
Push-push X band GaInP/GaAs VCO with a fully monolithic microstrip resonator (2004)

In this paper the design of a VCO using GaInP/GaAs HBT technology is presented. The VCO is designed to be a part of a PDH point to point radio system. To achieve low phase noise performances GaInP/GaAs HBT technology and push-push topology have been chosen. The MMIC includes predistorters to emphasize the second harmonic, f/sub 0//2 prescalers for PLL locking and buffer amplifiers. A fully monolithic microstrip resonator is coupled with integrated varactors to achieve the specified tuning bandwidth. Phase noise, bandwidth and power measurements will also be presented.
Lifetime Measurements of the [ITAL]E[/ITAL] [TSUP]1[/TSUP]Π, [FORMULA][F]v=0[/F][/FORMULA] and [FORMULA][F]v=1[/F][/FORMULA] States of [TSUP]12[/TSUP]C[TSUP]16[/TSUP]O, [TSUP]13[/TSUP]C[TSUP]16[/TSUP]O, and [TSUP]13[/TSUP]C[TSUP]18[/TSUP]O (1998)

In a laboratory investigation using tunable picosecond lasers in a pump-probe configuration, lifetimes of the E (II)-I-1, v = O and v = 1 states of CO are experimentally determined. For E (II)-I-1, v = 1, the lifetime is found to vary significantly for the isotopomers. Combining the data with existing oscillator strengths, predissociation probabilities within the range of 93.6%-98.64% result for (CO)-C-12-O-16, (CO)-C-13-O-16, and (CO)-C-13-O-18 in, the case of the E (II)-I-1, v=1 state, which is the state that predominantly influences the isotopic fractionation of CO in interstellar clouds.
Ubiquity of polystyrene digestion and biodegradation within yellow mealworms, larvae of Tenebrio molitor Linnaeus (Coleoptera: Tenebrionidae) (2018)

Academics researchers and “citizen scientists” from 22 countries confirmed that yellow mealworms, the larvae of Tenebrio molitor Linnaeus, can survive by eating polystyrene (PS) foam. More detailed assessments of this capability for mealworms were carried out by12 sources: five from the USA, six from China, and one from Northern Ireland. All of these mealworms digested PS foam. PS mass decreased and depolymerization was observed, with appearance of lower molecular weight residuals and functional groups indicative of oxidative transformations in extracts from the frass (insect excrement). An addition of gentamycin (30 mg g), a bactericidal antibiotic, inhibited depolymerization, implicating the gut microbiome in the biodegradation process. Microbial community analyses demonstrated significant taxonomic shifts for mealworms fed diets of PS plus bran and PS alone. The results indicate that mealworms from diverse locations eat and metabolize PS and support the hypothesis that this capacity is independent of the geographic origin of the mealworms, and is likely ubiquitous to members of this species.
Structure‐Directed Synthesis of new organic materials (1989)

The synthesis of molecular cages, belts and strips opens up new areas of technology for exploitation. Solvent specific sensors are one possible use of cage molecules called carcerands (see figure). A series of Diels Alder reactions under tight stereochemical control are used in a remarkably efficient manner to produce the structures. magnified image