gebhart/patrepeval huggingface dataset:
Towards a More Comprehensive Picture of the MicroRNA-23a/b-3p Impact on Impaired Male Fertility (2023)

The expression levels of various genes involved in human spermatogenesis are influenced by microRNAs (miRNAs), specifically microRNA-23a/b-3p. While certain genes are essential for spermatogenesis and male germ cell function, the regulation of their expression remains unclear. This study aimed to investigate whether microRNA-23a/b-3p targets genes involved in spermatogenesis and the impact of this targeting on the expression levels of these genes in males with impaired fertility. In-silico prediction and dual-luciferase assays were used to determine the potential connections between microRNA-23a/b-3p overexpression and reduced expression levels of 16 target genes. Reverse transcription-quantitative PCR (RT-qPCR) was conducted on 41 oligoasthenozoospermic men receiving infertility treatment and 41 age-matched normozoospermic individuals to verify the lower expression level of target genes. By employing dual-luciferase assays, microRNA-23a-3p was found to directly target eight genes, namely <i>NOL4, SOX6, GOLGA6C, PCDHA9, G2E3, ZNF695, CEP41</i>, and <i>RGPD1</i>, while microRNA-23b-3p directly targeted three genes, namely SOX6, <i>GOLGA6C</i>, and <i>ZNF695</i>. The intentional alteration of the microRNA-23a/b binding site within the 3' untranslated regions (3'UTRs) of the eight genes resulted in the loss of responsiveness to microRNA-23a/b-3p. This confirmed that <i>NOL4, SOX6, GOLGA6C, PCDHA9</i>, and <i>CEP41</i> are direct targets for microRNA-23a-3p, while <i>NOL4, SOX6</i>, and <i>PCDHA9</i> are direct targets for microRNA-23b-3p. The sperm samples of oligoasthenozoospermic men had lower expression levels of target genes than age-matched normozoospermic men. Correlation analysis indicated a positive correlation between basic semen parameters and lower expression levels of target genes. The study suggests that microRNA-23a/b-3p plays a significant role in spermatogenesis by controlling the expression of target genes linked to males with impaired fertility and has an impact on basic semen parameters.
Supplementary Figures 1-7, Table 1 from The Wnt Pathway Inhibitor RXC004 Blocks Tumor Growth and Reverses Immune Evasion in Wnt Ligand–dependent Cancer Models (2023)

&lt;p&gt;Supplementary Figure 1: Effect of RXC004 on gene expression, caspase activity and cell growth in colorectal and pancreatic cancer cell lines in vitro. Supplementary Figure 2: RXC004 effects on gene expression in in vivo xenograft tumor models. Supplementary Figure 3: Pharmacokinetic/pharmacodynamic (PK/PD) analysis of RXC004 in SNU-1411/NOD-SCID mice. Supplementary Figure 4: Dose-dependent effects of RXC004 on tumor markers, intestinal Ki67 and bodyweight. Supplementary Figure 5: Effect of RXC004 with or without anti-PD-1 on body weight and survival in in vivo models. Supplementary Figure 6: Dose-dependent effects of RXC004 alone or in combination with anti-CTLA-4 in B16F10/C57BL/6 syngeneic model. Supplementary Figure 7: In vitro PBMC co-culture additional cytokines and monocultures. Supplementary Table 1: In vivo exposure data for RXC004.&lt;/p&gt;
Masked Modeling Duo: Learning Representations by Encouraging Both Networks to Model the Input (2022)

Masked Autoencoders is a simple yet powerful self-supervised learning method. However, it learns representations indirectly by reconstructing masked input patches. Several methods learn representations directly by predicting representations of masked patches; however, we think using all patches to encode training signal representations is suboptimal. We propose a new method, Masked Modeling Duo (M2D), that learns representations directly while obtaining training signals using only masked patches. In the M2D, the online network encodes visible patches and predicts masked patch representations, and the target network, a momentum encoder, encodes masked patches. To better predict target representations, the online network should model the input well, while the target network should also model it well to agree with online predictions. Then the learned representations should better model the input. We validated the M2D by learning general-purpose audio representations, and M2D set new state-of-the-art performance on tasks such as UrbanSound8K, VoxCeleb1, AudioSet20K, GTZAN, and SpeechCommandsV2. We additionally validate the effectiveness of M2D for images using ImageNet-1K in the appendix.
The utility of fast active messages on many-core chips: Efficient supercomputing project (2011)

This article consists of a collection of slides from the author's conference presentation on the deployment of many core chips for supercomputing applications. In the many core era, power has become the limiting factor in performance scaling. This poster demonstrates the ability of active messages to increase the energy efficiency of parallel code.
Magneto-optical trapping of Yb atoms using an intercombination transition with an optical frequency comb: For realizing an Yb optical lattice clock (2008)

We have magneto-optically trapped cold ytterbium atoms using an intercombination transition for realizing an optical lattice clock. For this purpose, we have developed a laser-diode-based light source, which is phase-locked to an optical frequency comb, thus attaining both laser frequency stabilization and laser linewidth narrowing.
From Human-to-Human Touch to Peripheral Nerve Responses (2019)

Human-to-human touch conveys rich, meaningful social and emotional sentiment. At present, however, we understand neither the physical attributes that underlie such touch, nor how the attributes evoke responses in unique types of peripheral afferents. Indeed, nearly all electrophysiological studies use well-controlled but non-ecological stimuli. Here, we develop motion tracking and algorithms to quantify physical attributes - indentation depth, shear velocity, contact area, and distance to the cutaneous sensory space (receptive field) of the afferent - underlying human-to-human touch. In particular, 2-D video of the scene is combined with 3-D stereo infrared video of the toucher's hand to measure contact interactions local to the receptive field of the receiver's afferent. The combined and algorithmically corrected measurements improve accuracy, especially of occluded and misidentified fingers. Human subjects experiments track a toucher performing four gestures - single finger tapping, multi-finger tapping, multi-finger stroking and whole hand holding - while action potentials are recorded from a first-order afferent of the receiver. A case study with one rapidly-adapting (Pacinian) and one C-tactile afferent examines temporal ties between gestures and elicited action potentials. The results indicate this method holds promise in determining the roles of unique afferent types in encoding social and emotional touch attributes in their naturalistic delivery.
Inhibition of Protein–Protein Interactions by Peptide Mimics (2010)

This chapter contains sections titled: Introduction Inhibition of Calmodulin Inhibition of HIV-1 Fusion Inhibition of the Nuclear Estrogen Receptor Inhibition of the Bcl-xL/Bak Interaction Inhibition of the p53/MDM2 Interaction Miscellaneous Protein Targets Conclusion References
Porphyrin based metal–organic frameworks: highly sensitive materials for optical sensing of oxygen in gas phase (2021)

Luminescence of PCN-224-based MOFs is efficiently quenched by molecular oxygen making them promising for optical sensing of oxygen in the gas phase.
Data from Microparticle Encapsulation of a Prostate-targeted Biologic for the Treatment of Liver Metastases in a Preclinical Model of Castration-resistant Prostate Cancer (2023)

&lt;div&gt;Abstract&lt;p&gt;PRX302 is a highly potent, mutant bacterial pore-forming biologic protoxin engineered for selective activation by PSA, a serine protease expressed by benign and malignant prostate epithelial cells. Although being developed as a local therapy for benign prostatic hyperplasia and localized prostate cancer, PRX302 cannot be administered systemically as a treatment for metastatic disease due to binding to ubiquitously expressed glycosylphosphatidylinositol (GPI)-anchored proteins, which leads to poor accumulation within the tumor microenvironment. To overcome this limitation, poly-lactic-co-glycolic acid (PLGA) microparticles encapsulating the protoxin were developed, which are known to accumulate in the liver, a major site of metastasis for prostate cancer and other solid tumors. A highly sensitive and reproducible sandwich ELISA to quantify PRX302 released from microparticles was developed. Utilizing this assay, PRX302 release from different microparticle formulations was assessed over multiple days. Hemolysis assays documented PSA-dependent pore formation and lytic potential (i.e., function) of the released protoxin. MTT assays demonstrated that conditioned supernatant from PRX302-loaded, but not blank (i.e., unloaded), PLGA microparticles was highly cytotoxic to PC3 and DU145 human prostate cancer cells in the presence of exogenous PSA. Microparticle encapsulation prevented PRX302 from immediately interacting with GPI-anchored proteins as demonstrated in a competition assay, which resulted in an increased therapeutic index and significant antitumor efficacy following a single dose of PRX302-loaded microparticles in a preclinical model of prostate cancer liver metastasis with no obvious toxicity. These results document that PRX302 released from PLGA microparticles demonstrate &lt;i&gt;in vivo&lt;/i&gt; antitumor efficacy in a clinically relevant preclinical model of metastatic prostate cancer.&lt;/p&gt;&lt;/div&gt;
Virtual worlds, real sounds (2002)

This marks the first issue of IEEE Computer Graphics and Applications devoted entirely to sound. It might seem that sound and graphics are totally distinct fields with their own science, techniques, language, conferences, and publication venues, but the relationship between sound and graphics is actually natural. In fact, the relations and dependencies are as natural as the complementary senses of vision and audition.
Integriertes Skills-Lab-Konzept für die studentische Ausbildung in der Inneren Medizin (2005)

This pilot study shows that curriculum changes promoting basic clinical skills are effective and lead to an improved practical education of tomorrow's physicians. The integrated skills laboratory concept is well accepted and leads to a relevant increase in competence in the practice of internal medical. The presented skills laboratory concept in internal medicine is proving to be a viable and efficient learning tool.
A new germacranolide-type sesquiterpene lactone fromTanacetum santolinoides (2007)

A new germacranolide-type sesquiterpene lactone, 1alpha-hydroxy-3-oxo-7alpha,11betaH-germacra-4Z,9Z-dien-12,6alpha-olide (1), was isolated from the CH2Cl2--MeOH extract of the aerial parts of Tanacetum santolinoides. Its structure was determined by spectroscopic techniques including, IR, high-resolution-EIMS, and extensive 400 MHz one- and two-dimensional NMR-analysis (1H, 13C-NMR, DEPT, 1H--1H COSY, HMQC, HMBC, and NOE experiments).
Reduction of 1+1 resonance enhanced MPI spectra to populations and alignment factors (1986)

A theory is presented to reduce 1+1 resonance enhanced multiphoton ionization (REMPI) spectra to accurate rovibrational state population distributions. Classical and quantum mechanical treatments are developed to model the polarization dependence of the REMPI signal from an initially aligned ground state having cylindrical symmetry. The theory includes the effects of saturation and intermediate state alignment. It is demonstrated that, for favorable cases, 1+1 REMPI allows the determination of the relative population as well as the quadrupole and hexadecapole moments of the alignment for rovibrational levels of a linear molecule. The classical treatment differs from that of the quantum treatment by less than 5% for rotational quantum numbers greater than J=4, suggesting that the classical treatment suffices for 1+1 REMPI in most molecular systems.
Formal Parameterization of Log Synchronization Events within a Distributed Forensic Compute Cloud Database Environment (2012)

Advances in virtual server internetworking and the Internet have been accompanied by increased incidences of computer related crimes for such domains. At the same time, the number of sources of potential evidence in any particular cloud computing forensic investigation has grown considerably, as evidence of the occurrence of relevant events can potentially be drawn not only from multiple computers, networks, and electronic systems but also from disparate personal, organizational, and governmental contexts. Potentially, this leads to significant improvements in forensic outcomes but is accompanied by an increase in complexity and scale of the event information, particularly since such information is treated as composite events. In order for digital investigators to effectively administer the virtual machine(VM) environments they need to have automated methods for correlating and synchronizing such event data as a critical concern. The contribution of the paper is the provision of a University case study of our ongoing work that integrates an automated detection of a computer forensic scenario for virtual network server clouds. This is work based upon facts derived from digital events synchronized within the VM environment. We use our preliminary case evaluations to present the formal parameterized context for which such VM log events are likely to occur based on the event condition action (ECA) paradigm adopted from work done in [16][19].
Metallic and bimetallic nanocatalysts incorporated into highly porous coordination polymer MIL-101 (2009)

This paper reports the development of a facile, general and effective approach, based on microwave irradiation (MWI), for the incorporation of a variety of metallic and bimetallic nanoparticle catalysts within the highly porous coordination polymer MIL-101. The current approach is based on the simultaneous activation of the pores of MIL-101 and the rapid chemical reduction of metal precursors using MWI in the presence of a reducing agent. Small Pd, Cu and Pd–Cu nanoparticles of 2–3 nm are incorporated within the pores and larger particles of 4–6 nm are supported on the surface of the MIL-101 crystals. TEM images reveal that the loading of the particles using MWI is uniform across the MIL crystals. The observed catalytic activities toward CO oxidation of the Pd nanocatalysts supported on the highly porous MIL-101 polymer are significantly higher than any other reported metal clusters supported on metal–organic frameworks. The observed high activity is attributed to the small metal nanoparticles imbedded within the pores of the MIL crystals. The activity of the small embedded particles is higher than those supported on the surface. This allows the use of small metal loadings for efficient low temperature CO oxidation. These results should allow optimization of a new class of nanocatalysts incorporated within the highly porous MIL-101. These materials are promising environmentally relevant catalyst systems.
International Textbook of Diabetes Mellitus (2003)

Diagnosis, Epidemiology and Aetiology of Diabetes Biochemistry and Pathophysiology of Diabetes Management of Diabetes Monitoring of Therapy Special Problems in Management Acute Disturbances of Diabetes Chronic Complications of Diabetes Diabetes and Public Health.
O banco de dados do CRITT para a pesquisa do processo tradutório (2018)

Desde sua criação, há dez anos, o Center for Research and Innovation in Translation and Translation Technology (CRITT), da Copenhagen Business School (CBS), Dinamarca, está envolvido em pesquisas do processo tradutório. Os dados dessas pesquisas foram coletados inicialmente pela ferramenta Translog e publicados em 2012 como um banco de dados das pesquisas do processo tradutório (em inglês, Translation Process Research Database, ou TPR-DB). Desde 2012, outros experimentos foram realizados, e mais dados foram adicionados ao TPR-DB. Em particular, dentro do projeto CASMACAT – do inglês, Cognitive Analysis and Statistical Methods for Advanced Computer Aided Translation (SANCHIS-TRILLES et al., 2014) –, foi gravada uma grande quantidade de dados do processo de pós-edição de tradução automática, e o TPR-DB foi disponibilizado sob uma licença Creative Commons. No momento da redação deste artigo, o TPR-DB contém quase 30 estudos com tarefas de tradução, pós-edição, escrita (autoral) e/ou cópia, todas gravadas com o Translog e/ou com a ferramenta CASMACAT. Cada estudo é constituído por cerca de oito a mais de 100 sessões de gravação, envolvendo mais de 300 tradutores. Atualmente, os dados somam mais de 500 horas de produção textual reunidas em mais de 1.400 sessões, com mais de 600.000 palavras traduzidas para mais de dez línguas-alvo diferentes. Este artigo descreve os recursos e as opções de visualização do TPR-DB. Esse banco de dados contém registros do processo, bem como informações derivadas e anotadas organizadas em sete tipos de unidades simples e compostas do processo e do produto, adequadas para investigar os processos de tradução humana e assistida por computador, bem como para realizar modelagem avançada dos usuários.&#x0D; &#x0D; Palavras-chave: pesquisa empírica do processo tradutório, banco de dados de pesquisas do processo tradutório.
69.3: Polymer/Metal‐Oxide Composite: A Novel Buffer Layer for Solution‐Processible OLEDs (2007)

Abstract We developed a novel polymer‐metal oxide composite which is suitable as a buffer layer for solution‐processible OLEDs. The composite, which consists of vanadium oxide and an amine‐containing polymer, was fabricated by a sol‐gel technique. It is possible to create Ohmic contact between the composite and electrodes, and this enabled the production of OLEDs with low driving voltage and high current efficiency.
Effect of Dentinal Adhesives on Marginal Adaptation and Cavity Sealing with Resin Restorations In Vitro (1992)

In a companion article in this issue and a previous article in this journal, two experimental enamel dentin adhesive systems were evaluated and compared with four commercially available resin bonding agents by SEM investigation of the tooth adhesive interfaces and by determination of bond strengths to enamel and dentin under various conditions. In continuation of these screening investigations, the present article describes the results of cavity tests for assessment of marginal adaptation by measurement of gap dimensions, and of cavity sealing by rating of microleakage, when standardized dentin cavities were treated with the adhesive restorative systems. Both tests resulted in the same ranking of the six materials. Gluma, Clearfil Photo Bond, and two experimental compounds showed very narrow gaps and moderate microleakage. In contrast, Prisma Universal Bond 2 (the material has been replaced in the market by Prisma Universal Bond 3) and Scotchbond 2 showed significantly wider marginal gaps and more pronounced microleakage. Final discrimination between the efficacy of adhesive restorative materials by any of the currently used in vitro tests is disputable and prediction of long-term clinical performance by laboratory test data remains questionable.
Crowned Dendrimers: pH‐Responsive Pseudorotaxane Formation. (2003)

ChemInformVolume 34, Issue 26 Preparative Organic Chemistry Crowned Dendrimers: pH-Responsive Pseudorotaxane Formation. Jason W. Jones, Jason W. Jones Dep. Chem., Va. Polytech. Inst., State Univ., Blacksburg, VA 24061, USASearch for more papers by this authorWilliam S. Bryant, William S. Bryant Dep. Chem., Va. Polytech. Inst., State Univ., Blacksburg, VA 24061, USASearch for more papers by this authorAnton W. Bosman, Anton W. Bosman Dep. Chem., Va. Polytech. Inst., State Univ., Blacksburg, VA 24061, USASearch for more papers by this authorRene A. J. Janssen, Rene A. J. Janssen Dep. Chem., Va. Polytech. Inst., State Univ., Blacksburg, VA 24061, USASearch for more papers by this authorE. W. Meijer, E. W. Meijer Dep. Chem., Va. Polytech. Inst., State Univ., Blacksburg, VA 24061, USASearch for more papers by this authorHarry W. Gibson, Harry W. Gibson Dep. Chem., Va. Polytech. Inst., State Univ., Blacksburg, VA 24061, USASearch for more papers by this author Jason W. Jones, Jason W. Jones Dep. Chem., Va. Polytech. Inst., State Univ., Blacksburg, VA 24061, USASearch for more papers by this authorWilliam S. Bryant, William S. Bryant Dep. Chem., Va. Polytech. Inst., State Univ., Blacksburg, VA 24061, USASearch for more papers by this authorAnton W. Bosman, Anton W. Bosman Dep. Chem., Va. Polytech. Inst., State Univ., Blacksburg, VA 24061, USASearch for more papers by this authorRene A. J. Janssen, Rene A. J. Janssen Dep. Chem., Va. Polytech. Inst., State Univ., Blacksburg, VA 24061, USASearch for more papers by this authorE. W. Meijer, E. W. Meijer Dep. Chem., Va. Polytech. Inst., State Univ., Blacksburg, VA 24061, USASearch for more papers by this authorHarry W. Gibson, Harry W. Gibson Dep. Chem., Va. Polytech. Inst., State Univ., Blacksburg, VA 24061, USASearch for more papers by this author First published: 27 June 2003 https://doi.org/10.1002/chin.200326082AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat No abstract is available for this article. Volume34, Issue26July 1, 2003 RelatedInformation
Analogue MOS current-mode circuits for three-dimensional integrated smart image sensor (1990)

An image processing technique using analogue MOS current-mode circuits is presented. This approach is of interest in smart image sensors based on three-dimensional (or multi-layered) VLSI structures. High-performance smart image sensors with high resolution can be realised because the number of transistors required for image processing in each pixel is greatly reduced.
Cancer Cell Metabolism: One Hallmark, Many Faces (2012)

Cancer cells must rewire cellular metabolism to satisfy the demands of growth and proliferation. Although many of the metabolic alterations are largely similar to those in normal proliferating cells, they are aberrantly driven in cancer by a combination of genetic lesions and nongenetic factors such as the tumor microenvironment. However, a single model of altered tumor metabolism does not describe the sum of metabolic changes that can support cell growth. Instead, the diversity of such changes within the metabolic program of a cancer cell can dictate by what means proliferative rewiring is driven, and can also impart heterogeneity in the metabolic dependencies of the cell. A better understanding of this heterogeneity may enable the development and optimization of therapeutic strategies that target tumor metabolism. Significance: Altered tumor metabolism is now a generally regarded hallmark of cancer. Nevertheless, the recognition of metabolic heterogeneity in cancer is becoming clearer as a result of advancements in several tools used to interrogate metabolic rewiring and dependencies. Deciphering this context-dependent heterogeneity will supplement our current understanding of tumor metabolism and may yield promising therapeutic and diagnostic utilities.
Compelling Reasons for Repairing Human Germlines (2017)

Interview with Dr. George Church on recent advances in human germline repair and movement toward clinical applications. (07:01)Download Researchers recently reported that, using gene-editing technology, they reduced the proportion of embryos containing a dominant disease allele. Several key advances could result in useful deployment of a high-impact technology that would improve future human health.