gebhart/patrepeval huggingface dataset:
CD34‐derived dendritic cells transfected ex vivo with HIV‐Gag mRNA induce polyfunctional T‐cell responses in nonhuman primates (2012)

The pivotal role of DCs in initiating immune responses led to their use as vaccine vectors. However, the relationship between DC subsets involved in antigen presentation and the type of elicited immune responses underlined the need for the characterization of the DCs generated in vitro. The phenotypes of tissue-derived APCs from a cynomolgus macaque model for human vaccine development were compared with ex vivo-derived DCs. Monocyte/macrophages predominated in bone marrow (BM) and blood. Myeloid DCs (mDCs) were present in all tested tissues and were more highly represented than plasmacytoid DCs (pDCs). As in human skin, Langerhans cells (LCs) resided exclusively in the macaque epidermis, expressing CD11c, high levels of CD1a and langerin (CD207). Most DC subsets were endowed with tissue-specific combinations of PRRs. DCs generated from CD34(+) BM cells (CD34-DCs) were heterogeneous in phenotype. CD34-DCs shared properties (differentiation and PRR) of dermal and epidermal DCs. After injection into macaques, CD34-DCs expressing HIV-Gag induced Gag-specific CD4(+) and CD8(+) T cells producing IFN-γ, TNF-α, MIP-1β, or IL-2. In high responding animals, the numbers of polyfunctional CD8(+) T cells increased with the number of booster injections. This DC-based vaccine strategy elicited immune responses relevant to the DC subsets generated in vitro.
Liquid Chromatography–Tandem Mass Spectrometry in Newborn Screening Laboratories (2022)

Tandem mass spectrometry (MS/MS) is the most universal platform currently available for the analysis of enzymatic activities and biomarkers in dried blood spots (DBS) for applications in newborn screening (NBS). Among the MS/MS applications in NBS, the most common is flow-injection analysis (FIA-) MS/MS, where the sample is introduced as a bolus injection into the mass spectrometer without the prior fractionation of analytes. Liquid chromatography combined with MS/MS (LC-MS/MS) has been employed for second-tier tests to reduce the false-positive rate associated with several nonspecific screening markers, beginning two decades ago. More recently, LC-MS/MS has been applied to primary screening for new conditions for which FIA-MS/MS or other methods, including genomic screening, are not yet adequate. In addition to providing a list of the currently used LC-MS/MS-based assays for NBS, the authors share their experience regarding the maintenance requirements of LC-MS/MS vs. FIA-MS/MS systems. The consensus is that the maintenance of LC-MS/MS and FIA-MS/MS instrumentation is similar, and LC-MS/MS has the advantage of allowing for a larger number of diseases to be screened for in a multiplex, cost-effective fashion with a high throughput and an adequate turnaround time.
ChemInform Abstract: Novel Two‐Step, One‐Pot Synthesis of Primary Acylureas. (2011)

Abstract The process involves the BOP‐promoted coupling of carboxylic acids with cyanamide and subsequent mild hydrolysis.
The Image Torque Operator for Contour Processing (2016)

Contours are salient features for image description, but the detection and localization of boundary contours is still considered a challenging problem. This paper introduces a new tool for edge processing implementing the Gestaltism idea of edge grouping. This tool is a mid-level image operator, called the Torque operator, that is designed to help detect closed contours in images. The torque operator takes as input the raw image and creates an image map by computing from the image gradients within regions of multiple sizes a measure of how well the edges are aligned to form closed convex contours. Fundamental properties of the torque are explored and illustrated through examples. Then it is applied in pure bottom-up processing in a variety of applications, including edge detection, visual attention and segmentation and experimentally demonstrated a useful tool that can improve existing techniques. Finally, its extension as a more general grouping mechanism and application in object recognition is discussed.
Inv(11)(q21q23) Fuses MLL to the NOTCH Co-Activator Mastermind-Like 2 in Secondary T Cell Acute Lymphoblastic Leukemia. (2006)

Abstract Development of a secondary leukemia after chemotherapeutic treatment for childhood acute leukemia is associated with rearrangements of the MLL gene on chromosome 11q23 and characteristically results in acute myeloid leukemia (AML). We identified the NOTCH co-activator Mastermind-like 2 as MLL fusion partner in two cases of pediatric secondary leukemia. The MLL-MAML2 fusion results from a cryptic inv(11)(q21q23) only detectable by interphase fluorescence in situ hybridization with an MLL split signal probe. With a latency of twenty months and five years after initial diagnosis for MLL negative AML and precursor B acute lymphoblastic leukemia (ALL), respectively, both patients developed a therapy-related T-cell acute lymphoblastic leukemia. MAML2 is the first MLL fusion partner involved in human Notch-signaling and was only recently identified as recurrent translocation fusion partner in a subset of salivary gland tumors. The genomic MLL breakpoint shows similar localization and sequence features described for etoposide induced treatment-related AML. MLL-MAML2 positive cells were detectable up to two years prior to clinical apparent secondary leukemia in one case. The discrepant dynamics of clone expansion quantified by either the genomic fusion sequence or Ig/TCR gene rearrangements as clone specific markers suggests a selection process within the inversion positive population and the need for additional mutation events to promote overt leukemic disease. Whole genome expression profiles demonstrated differential expression of both typical MLL and NOTCH downstream genes, which suggests a modulatory role of the MAML2 transcriptional activation domain in MLL leukemogenesis and lineage assignment induced by the MLL-MAML2 fusion protein. MSM21620813 WSF3401600.
Age, Sex, and Remote Monitoring Differences in Device Acceptance for Patients With Implanted Cardioverter Defibrillators in Canada (2020)

ICD patients aged ≤ 65 years reported poorer device acceptance and greater shock anxiety than older patients. Younger patients may require targeted interventions addressing adjustment to the ICD, and impact of the ICD on body image. Moreover, education about the relatively low probability of shocks may alleviate shock anxiety in younger patients.
ENDOGENOUS VASOPRESSIN AND COPEPTIN RESPONSE IN MULTIPLE TRAUMA PATIENTS (2007)

Endogenous arginine vasopressin (AVP) levels in multiple trauma patients are unknown. Arginine vasopressin is considered to play an important role in severe hemorrhage. In this prospective study, 87 multiple trauma patients (Injury Severity Score >15) and 50 healthy volunteers were enrolled. On admission to the emergency department (ED), demographic, clinical, and laboratory data were documented, and blood was sampled for determination of AVP (radioimmunosassay) and copeptin, a stable fragment of the AVP precursor (immunoluminometric assay). In patients requiring intensive care unit (ICU) therapy, blood and data sampling were repeated at 4, 6, and 24 h after ED admission. Linear logistic and mixed-effects regression analyses were used for statistical analysis. On ED admission, AVP plasma concentrations (43.2 +/- 84.9 pM) were significantly increased when compared with controls (0.92 +/- 0.44 pM, P < 0.001). Plethysmographic oxygen saturation was the only parameter independently associated with AVP (regression coefficient, -0.126; 95% confidence interval, -0.237 to -0.014; P = 0.03). No correlation was observed between AVP and survival (P = 0.62), hemodynamic variables (systolic arterial pressure, P = 0.24; MAP, P = 0.59; diastolic arterial pressure, P = 0.74; central venous pressure, P = 0.36), or brain trauma (P = 0.46). In ICU patients, AVP decreased during the first 24 h (P < 0.001) and was independently associated with heart rate (P = 0.02) and blood glucose (P = 0.009). Copeptin concentrations were correlated with AVP (r2 = 0.718, P < 0.001). In conclusion, AVP was significantly increased in multiple trauma patients and seems to be an integral part of the neuroendocrine response to severe injury. In ICU patients, AVP decreased to moderately elevated levels within 24 h after ED admission.
Breakup of Heavy Fermions on the Brink of “PhaseA” inCeCu2Si2 (1998)

We report resistivity, $\ensuremath{\rho}(T)$, and specific-heat, $C(T)$, results on near stoichiometric $\mathrm{CeCu}{}_{2}{\mathrm{Si}}_{2}$ samples, in the vicinity of a quantum critical point (QCP). The latter is defined by ${T}_{A}\ensuremath{\rightarrow}0$, where ${T}_{A}\ensuremath{\lesssim}0.8\mathrm{K}$ marks the transition into a spin-density--wave-type ``phase $A$'' which competes with heavy-fermion superconductivity below ${T}_{c}\ensuremath{\approx}0.65\mathrm{K}$. Upon approaching the QCP, $\ensuremath{\rho}(T)$ and $C(T)$ behave very disparately, suggesting a breakup of the heavy quasiparticles. Very surprising observations are being made for samples with ${T}_{A}&gt;0$ also.
Synthesis of Isoquinolines and Pyridines by the Palladium- and Copper-Catalyzed Coupling and Cyclization of Terminal Acetylenes (1999)

3-Substituted isoquinolines have been synthesized by the coupling of aryl- and alkenyl-substituted terminal acetylenes with the tert-butylimine of o-iodobenzaldehyde in the presence of a palladium catalyst. Alkyl-substituted acetylenes fail to undergo this annulation process. However, the intermediate iminoalkynes containing aryl, alkenyl, and alkyl substituents produced from these palladium-catalyzed reactions undergo copper-catalyzed cyclization in excellent yields and short reaction times. Isoquinolines and pyridines have thus been prepared in a one-pot synthesis via coupling of aryl-, alkenyl-, and alkyl-substituted terminal acetylenes with the tert-butylimines of o-iodobenzaldehydes or 3-halo-2-alkenals in the presence of a palladium catalyst and subsequent copper-catalyzed cyclization of the resulting iminoalkynes. The total synthesis of the isoquinoline natural product decumbenine B has been accomplished in seven steps and 20% overall yield by employing this palladium-catalyzed coupling/cyclization methodology.
Distributed Trust Based Routing in Mobile Ad-Hoc Networks (2013)

Establishing trust and security in ad-hoc networks has been a long studied problem. Several methods have been proposed for evaluation and dissemination of trust in such networks, with the goal of providing secure data paths. In this paper, we propose a simple mechanism to utilize the point-to-point trust metrics derived from various methods for secure routing. The main advantage of our scheme is that it can be used in with existing on-demand routing protocols with minimal modification. Additionally, our scheme utilizes both link trust and the traditional node trust. We highlight scenarios that establish the need for associating trust with a link, rather than just a node. We validate via simulation, the security properties of our scheme in an ad-hoc network.
3D stacking using Cu-Cu direct bonding (2012)

Cu-Cu bonding provides advantages such as cost effective process, fine pitch, and low resistance to advanced 3D IC technology. For 3D stacking, TSV Cu nail can be revealed by wafer backside process and used as interconnect between top and bottom die. No extra process like RDL (Re-Distribution Layer) and bumping are required, thus, μbump pitch is equivalent to TSVs pitch. Further there is no inter-metallic compound (IMC) layer formation and thus improve the mechanical reliability. In this work, we report the results achieved for 3D staking with Cu-Cu bonding by using TCB (Thermal Compression Bonding) process. Three different TSVs nail structures named No Nail, Flat Surface, and Dome Shape, respectively are choose to study the impact of nail structures to various TCB conditions. The TSVs have 5μm diameter with a minimum pitch of 10μm. Cross section SEM (Scanning Electron Microscope) is used to identify interface-bonding qualities. Electrical characterizations on TSV chains are also performed. Based on above specifics results, a best performing structure for TSVs nails is selected in term of electrical connections and good adhesions between dies. Finally, we propose some suggestions for future work on Cu-Cu bonding.
Macrocyclization by Nickel‐Catalyzed, Ester‐Promoted, Epoxide–Alkyne Reductive Coupling: Total Synthesis of (−)‐Gloeosporone (2009)

Ringing the changes: The total synthesis of the title compound centers around a novel strategy that employs a nickel(0)-phosphine complex and triethyl borane in an efficient closure of a 14-membered ring through C--C bond formation (see scheme; cod=cyclooctadiene). The synthesis was accomplished in 10 steps and in approximately 9 % overall yield.
An image-based approach for designing and manufacturing craniofacial scaffolds (2000)

Bone tissue engineering (BTE), which combines biomaterial scaffolds with biologically active factors, holds tremendous promise for reconstructing craniofacial defects. A significant challenge in craniofacial reconstructive BTE applications is the complex patient-specific geometry that must be reconstructed. In this paper, we present an image-based approach for designing and manufacturing patient-specific craniofacial biomaterial scaffolds directly from CT or MRI data. In this approach, voxel density distribution is used to define scaffold topology. The scaffold design topology is created using image processing techniques. This voxel density distribution is then converted to data that can be used to drive a Solid Free-Form Fabrication machine to either directly build the scaffold or build a mold for the scaffold. Several preliminary applications for craniofacial surgery, including a mandibular condyle scaffold, an orbital floor scaffold, and a general mandibular defect scaffold, are illustrated. Finally, we show applications to in vivo models.
Video (2014)

This video presents a demo of indoor localization in multiple settings. In the demo, a user walks with a smartphone and the user's location is shown on the phone's screen in real time. Our system, called Unsupervised Indoor Localization (UnLoc) utilizes the sensor data from smartphones to learn "invisible landmarks" in the environment. Example landmarks could be a unique magnetic fluctuation experienced when the phone is near a water-cooler, or a distinct gyroscope rotation when the user turns a corner. We use these indoor "landmarks" to periodically reset the user's location. To track the user between these landmarks, we use an optimized variant of dead reckoning, ultimately leading to a robust location tracking system. We call our system UnLoc, since the landmarks are generated in an unsupervised manner, requiring no manual effort or floorplan of the building. The demo describes the high level intuitions, shows UnLoc in operation, and shares experiences from running UnLoc in various real-world environments.
Medium viscosity regulates the activity of membrane-bound and soluble phospholipase A2 (1987)

Medium viscosity is a regulator of very low density lipoprotein production by cultured hepatocytes; their secretion and synthesis are inversely proportional to the extracellular fluid viscosity. The possibility that the mechanism of this extracellular effect on cell function involves modulation of cell membrane component(s) was considered. Along with this assumption, we studied the effect of medium viscosity on the activity of phospholipase A2 (PLA2), an enzyme present in the cell surface membrane, and the activity has been correlated with cellular secretion. We have found that culture medium viscosity inhibits the activity of PLA2 in the plasma membrane of cultured liver cells, concomitantly with the inhibition of lysosomal enzyme and lipoprotein secretion. It was also found that the degradation of liposomal phosphatidylcholine by soluble snake venom PLA2 is inversely proportional to the solvent viscosity. The possibility that the effect of medium viscosity on the enzymatic reaction involves the modulation of dynamic properties of membrane phospholipids was then considered. This hypothesis was examined by monitoring the fluorescence depolarization of fluorophores incorporated into phospholipid vesicles. No significant effect of the solvent viscosity on the phospholipid bilayer was observed. It is proposed that the regulation of cellular secretion by extracellular fluid viscosity involves modulation of the cell membrane PLA2 activity.
A Case of Acute Myelogenous Leukemia Complicated with Multiple Liver Abscesses by Candida albicans (1986)

A case of acute myelogenous leukemia complicated with multiple liver abscesses by Candida albicans was reported. The patient was a 46-year-old Japanese woman, who was referred to Kyushu University Hospital because of severe anemia. Before admission, she was transfused more than 1, 000 ml of packed red blood cells in total. On admission, the number of bone marrow nucleated cells was 38 × 104/mm3, and 91% of them were peroxidase positive myeloblasts. During the course of remission inductiontherapy with BH-AC, daunorubicin, 6-mercaptopurine and prednisolone, she developed high fever which was unresponsive to various kinds of antibiotics. Twenty days after the onset of high fever, ultrasound tomography revealed multiple “bull's eye” lesions in the liver. Multiple liver abscesses were demonstrated by abdominal CT. Culture of the aspirate demonstrated Candida albicans in the lesion. Treatment with Amphotericin B of a total dose of 3800 mg for more than six monthsand drainage of some abscesses were ineffective. Eight months after her admission, acute myelogenous leukemia relapsed.
β2‐Adrenergic Receptor Activation by Agonists Studied with 19F NMR Spectroscopy (2013)

Proteine in Zeitlupe: 19F-NMR-Studien deuten darauf hin, dass Übergänge zwischen aktiven und inaktiven Zuständen des humanen β2-adrenergen Rezeptors erhebliche Strukturumwandlungen erfordern (siehe Bild). Dies zeigt sich an der Enthalpiedifferenz ΔHo≈40 kJ mol−1 und einer langsamen Umwandlungsgeschwindigkeit mit kex≪10 s−1.
Lack of correlation between ATM protein expression and tumour cell radiosensitivity (1998)

Variations in the levels of ATM protein are insufficient to explain the differences in cellular radiosensitivity observed in a panel of human malignant glioma cell lines.
A Survey of Cocrystals Reported Prior to 2000 (2009)

A history of cocrystals reported in the literature prior to the year 2000 is presented. Concentration is on cocrystals that contain only organic components, not including species commonly referred to as solvates and clathrates. However, brief mention is made of some cocrystals containing both organic and inorganic components. The discovery and early history of cocrystals are discussed, with emphasis on centers of activity. Numerous examples are then utilized to illustrate the structural variety and utility of cocrystals described in the literature.
Poynting Theory for Cable Wound Generators (2006)

Production and consumption of electricity have grown enormously during the last century. No mater what the primary source of energy is, almost all generation of electricity comes from conversion of a rotational movement in a generator. The aim of this thesis is to see how high voltage technology influence production and storage of electricity. Power flow in the generators used to convert mechanical movement to electric energy is analyzed using Poynting‘s vector. The impact of new generator technology for efficient extraction of hydroelectric power is shown. Simulation of a large permanent magnet turbo generator is presented. A flywheel storage system for electric vehicles utilizing high voltage technology is also presented. In pulsed power applications, a cheap method for intermediate storage of energy during milliseconds, which enables an inductive primary storage is presented and experimentally tested.
A COMPACT INTEGRATED HYPERSPECTRAL IMAGER MADE BY GRAY-TONE LITHOGRAPHY (2014)

Optical elements of precise thickness profiles are desired for instruments such as coronagraphs [1], wavefront coding systems [2], and compact hyperspectral imagers Lack of subwavelength precision in the fabrication of these optical elements has required additional optical components, reducing the manufacturing yield and degrading performance. Here, we demonstrate a fabrication technique allowing for the control of surface fabrication errors of less than 1 nm and use this technique to fabricate a compact hyperspectral imager with the capability of high yield manufacturing.
410 Investigation of the flow field near a moving contact line on a heated vertical wall using temperature-sensitive liquid crystal particles (2013)

The Japan Soclety of Mechanlcal Englneers 410
A novel IgM intranasal intervention against SARS-CoV-2 (2021)

Statement of Significance: IgM and IgA constitute the first line of defense in the respiratory tract against mucosal pathogens. These immunoglobulins assemble into pentamers and dimers respectively. A recent publication in focus highlights the discovery of IgM-14, a novel IgM molecule for prophylactic as well as therapeutic treatment against the emerging SARS-CoV-2 variants.
Graether pupil expander for managing the small pupil during surgery (1996)

The Graether pupil expander, a device for mechanically dilating the pupil, is a soft silicone ring grooved to engage the iris sphincter and maintain pupil dilation during cataract surgery and intraocular lens implantation. The ring is incomplete; the gap is bridged by a slender strap that provides access to the pupil aperture. The pupil expander will be preloaded on a disposable insertion tool that permits the expander to be inserted through the primary surgical incision. Stretching the pupil and placing the pupil expander is facilitated by an iris glide-retractor that fixates the iris sphincter at the incision prior to insertion of the pupil expander. Clinical experience with over 100 cases is briefly discussed.
2,3‐Dimethyl‐1,4,8,11‐Tetraazacyclotetradeca‐1,3‐Diene(2,3‐Me 2 [14]‐1,3‐diene‐1,4,8,11‐N 4 ) Complexes (1978)

This chapter contains sections titled: (2,3-Dimethyl-1,4,8,11-tetraazacyclotetradeca-1,3-diene)nickel(II)Tetrachlorozincate(2-) Dibromo(2,3-dimethyl-1,4,8,11-tetraazacyclotetradeca-1,3-diene)-cobalt(III)Perchlorate
Resonant-enhanced localized surface plasmon resonance spectroscopy (2006)

The extinction maximum of the localized surface plasmon resonance (LSPR) of noble metal nanoparticles is highly dependent upon the refractive index of the nanoparticles' surrounding environment. In this study, the effect that molecular resonances have on the intensity, LSPR peak width, and LSPR shift of the LSPR of Ag nanoparticles is monitored. By systematically tuning the LSPR extinction maxima of Ag nanoparticles versus molecular resonances, new phenomena are revealed. First, the LSPR peak shift induced by a resonant molecule varies with wavelength. In most instances, the trends in this data qualitatively track with the Kramer's-Kronig transformation of the molecular resonance spectrum; however, the magnitude of the response is severely underestimated. This was verified from both experimental data and theoretical calculations. Because this phenomenon is revealed to be electronic transition dependent, it is hypothesized that the coupling between the molecular and plasmon resonances is responsible for this wavelength dependent observation. These results will have implications in molecular enhanced LSPR sensing and in the understanding of surface-enhanced spectroscopy.