gebhart/patrepeval huggingface dataset:
EZH2 is a mediator of EWS/FLI1 driven tumor growth and metastasis blocking endothelial and neuro-ectodermal differentiation (2009)

Ewing Tumors (ET) are highly malignant, localized in bone or soft tissue and are molecularly defined by ews/ets translocations. DNA microarray analysis revealed a relationship of ET to both endothelium and fetal neural crest. We identified expression of histone methyl-transferase Enhancer of Zeste, Drosophila, Homolog 2 (EZH2) to be increased in ET. EZH2's suppressive activity maintains stemness in normal and malignant cells. Here, we found EWS/FLI1 bound to the EZH2 promoter in vivo and induced EZH2 expression in ET and mesenchymal stem cells. Down-regulation of EZH2 by RNA interference in ET suppressed oncogenic transformation by inhibiting clonogenicity in vitro. Similarly, tumor development and metastasis was suppressed in immunodeficient Rag2-/-γC-/- mice. EZH2-mediated gene silencing was shown to be dependent on histone deacetylase (HDAC) activity. Subsequent microarray analysis of EZH2 knock down, HDAC-inhibitor treatment and confirmation in independent assays revealed an undifferentiated phenotype maintained by EZH2 in ET. EZH2 regulated stemness genes such as nerve growth factor receptor (NGFR) as well as genes involved in neuroectodermal and endothelial differentiation (EMP1, EPHB2, GFAP, GAP43). These data suggest that EZH2 might play a central role in Ewing Tumor pathology by shaping the oncogenicity and stem cell phenotype of this tumor.
Sympathetic Effects of Internal Carotid Nerve Manipulation on Choroidal Vascularity and Related Measures (2019)

Modulation of ICN activity reveals a potential role of the ocular sympathetic system in regulating endpoints related to neovascular diseases of the eye.
UNALTERED IBUPROFEN-INDUCED FAECAL BLOOD LOSS UPON COADMINISTRATION OF MOCLOBEMIDE (1992)

The influence of moclobemide on ibuprofen-induced faecal blood loss was investigated in 24 volunteers. The subjects were randomly assigned to one of two groups and received from day 1 until day 14 either moclobemide 150 mg t.i.d. (group A) or placebo t.i.d. (group B). On days 8-14, when moclobemide concentrations in group A were at steady state, all volunteers additionally received ibuprofen (600 mg t.i.d). From day 15 to 21, all subjects received placebo alone. Faecal blood loss (FBL) was quantified daily by the 51Cr-labelled erythrocyte method. As expected for ibuprofen, a significant increase in FBL during the second week of the study was observed. There was no difference in FBL between the two treatment groups (moclobemide or placebo). Similar FBL values were observed in both groups (group A vs B): during the first week the FBL values were (mean +/- SD) 0.40 +/- 0.23 ml/day vs 0.55 +/- 0.53 ml/day on days 1-3 and 0.40 +/- 0.21 ml/day vs 0.37 +/- 0.13 ml/day on days 4-7. The increase in FBL during the second week was comparable in both groups, with and without moclobemide (days 8-10: 0.78 +/- 0.59 ml/day vs 0.80 +/- 0.58 ml/day; days 11-14: 1.49 +/- 0.95 ml/day vs 1.28 +/- 0.62 ml/day). A decline in FBL was observed during the third week under placebo in both groups, but baseline values were not reached during the observation period. Again there was no difference between the two groups (days 15-17: 0.91 +/- 0.52 ml/day vs 0.92 +/- 0.47 ml/day; days 18-21: 0.74 +/- 0.30 ml/day vs 0.68 +/- 0.48 ml/day). No statistically significant interaction was found between week and type of treatment, indicating that no significant influence of moclobemide on the ibuprofen-induced faecal blood loss occurred. No notable pharmacokinetic interaction between moclobemide and ibuprofen was observed. Moclobemide plasma concentration-time profiles with and without concomitantly administered ibuprofen were superimposable. The results demonstrate that the concomitant administration of ibuprofen and moclobemide to healthy volunteers does not result in a clinically significant interaction, either at the pharmacodynamic (faecal blood loss) or at the pharmacokinetic level.
<title>Development of a high-speed PtSi IRCCD camera system</title> (1992)

This paper describes the construction of a high-speed PtSi infrared charge-coupled device (IRCCD) camera system funded by the U.S. Army Tank-Automotive Command (TACOM) for recording periodic events in the infrared. A brief discussion of PtSi infrared detection, and an analysis of expected signal contrast and NE(Delta) T of the device are presented. Integration times to less than 1 ms and the ability to record frames based on an external trigger allow this setup to achieve effective frame rates of over 1000 frames per second. The use of a personal computer (PC) as a controller for CCD clocking and video capture results in a flexible camera system design.
Therapeutic implications of enhanced G(0)/G(1) checkpoint control induced by coculture of prostate cancer cells with osteoblasts. (2001)

Osteoblastic metastases are common in lethal prostate cancer. Effective therapy for bone metastases is lacking. Thus, developing an appropriate in vitro screening system is critical to prioritize which of the newly developed agents should undergo additional expensive and time-consuming in vivo evaluation in bone metastases animal models. In the past, such in vitro screening evaluated the response of prostate cancer cells to chemotherapeutic agents in monoculture without the presence of osteoblasts. In such monoculture, prostate cancer cells have a high (i.e., >90%) proliferative growth fraction. In contrast, the growth fraction (i.e., mean: 7.1 +/- 0.8%; median: 3.1%) in 117 metastatic sites of prostate cancer obtained from 11 androgen ablation failing patients at "warm" autopsy was found to be >10-fold lower. To better mimic the lower growth fraction observed clinically, LNCaP human prostate cancer cells were cocultured with membrane-separated hFOB human osteoblasts. Such coculturing significantly lowered the growth fraction of the LNCaP cells (i.e., from >90 to <30%) without enhancing their low rate (i.e., <5%) of apoptosis. This lowering of the growth fraction was documented using flow cytometry, Ki-67 immunohistochemistry, and 5-bromo-2-deoxyuridine incorporation. Using RNase protection assays, it was documented that coculture with osteoblasts causes enhanced p53, p27, and p21 expression leading to a decrease in the number of LNCaP cells entering the cell cycle (i.e., enhanced number of LNCaP cells in G(0)-G(1) and a decrease in S and G(2)-M and thus the growth fraction). This osteoblast-induced enhanced G(0)-G(1) checkpoint control affected the chemosensitivity of LNCaP cells. This was documented by coculturing LNCaP cells with hFOB cells to condition the medium for 3 days to lower the growth fraction to <30% before exposing the LNCaP cells for 48 h to various concentrations of Taxol, doxorubicin, or thapsigargin (TG). In standard high (i.e., >90%) growth fraction cultures (i.e., cultures in the absence of osteoblast-conditioned medium), there was a dose-dependent and significant (P < 0.05) increase in apoptosis of LNCaP cells exposed to Taxol or doxorubicin. In contrast, even the highest dose of Taxol (1 microM) did not enhance apoptosis of lower growth fraction LNCaP cells cultured in osteoblast-conditioned medium. Similarly, only the highest concentration of doxorubicin (1 microM) enhanced apoptosis in lower growth fraction cells. In contrast, 100 nM TG induced high levels of apoptosis in both lower and high-growth fraction LNCaP cultures. These results demonstrate that the osteoblast/LNCaP coculture system is a better in vitro screen than monoculture to identify proliferation-independent agents for the treatment of prostate cancer bone metastases, and TG is such an agent.
RECONSTITUTION OF HUMAN CORPORAL SMOOTH MUSCLE AND ENDOTHELIAL CELLS IN VIVO (1999)

The availability of autologous erectile tissue composed of corporal smooth muscle and endothelial cells would be beneficial in patients undergoing penile reconstruction. We previously showed that cultured cavernous cells seeded on polymer scaffolds form corporal muscle when implanted in vivo. However, to reconstruct corporal tissue endothelial and corporal muscle cells are necessary. In this study we investigated the possibility of developing tissue composed of corporal cells in vivo by combining smooth muscle and endothelial cells.Human corporal smooth muscle and endothelial cells were seeded on biodegradable polyglycolic acid polymer scaffolds at concentrations of 20 x 10(6) and 10 x 10(6) cells per cm3, respectively. A total of 60 polymer scaffolds seeded with cells and 20 control polymers without cells were implanted in the subcutaneous space of 20 athymic mice. Mice were sacrificed 1, 3, 5, 7, 14, 21, 28 and 42 days, respectively, after implantation. Immunocytochemical and histochemical analyses were performed with antifactor VIII, antipancytokeratins and anti-alpha actin antibodies.Histologically the retrieved polymers seeded with corporal smooth muscle and endothelial cells showed the formation of multilayered smooth muscle strips adjacent to endothelial cells 7 days after implantation. Increased organization of the smooth muscle tissue and accumulation of endothelium lining the luminal structures were evident by 14 days. A well organized tissue construct was noted 28 and 42 days after implantation. There was no evidence of tissue formation in controls. Immunocytochemical analysis using antifactor VIII to identify native vasculature only and antipancytokeratins to identify ECV 304 endothelial cells only distinguished the origin of the vascular structures in each construct. Anti-alpha-actin confirmed the smooth muscle phenotype.Human corporal smooth muscle and endothelial cells seeded on biodegradable polymer scaffolds formed vascularized corpus cavernosum muscle when implanted in vivo. To our knowledge this is the first demonstration in tissue engineering in which capillary formation was facilitated by the addition of endothelial cells in composite tissue in vivo.
An Efficiency Dependency Study on the Heat Management of the A-CAES Plant with Liquid Heat Medium Loops (2020)

The simulation results of the heat medium control and management of A-CAES plant (Adiabatic Compressed Air Energy Storage) are presented. For the study, the dynamic model of the A-CAES plant with liquid heat medium as heat storage is constructed in order to analyze charge-discharge cycle efficiency dependency on actual operating conditions. Various heat management systems for a CAES are proposed. Among the systems, heat medium circulating system is the most feasible solution. The heat management and storage is very important for the A-CAES plant, because about half of the energy is recovered through heat insulation and heat management. Dynamic plant efficiency analysis is also crucial because the dynamic properties of the plant influences on the efficiency. The model consists of the heat exchangers, the heat storages, the air storage and an ideal compressor and expander models. The heat transfer characteristics of the heat exchanger are tested by the experimental setup because the heat exchanger performance is the most important factor for the system efficiency. The plant scale and the heat loss influence on the charge-discharge efficiencies are shown through the simulations.
Dynamic Nonlinear Systems (2015)

This chapter discusses the impact of the presence of memory effects on the behavior of nonlinear systems. The different types of memory effects are discussed and models to describe systems with memory are covered.
Potts Ferromagnets on Coexpressed Gene Networks: Identifying Maximally Stable Partitions (2003)

Clustering gene expression data by exploiting phase transitions in granular ferromagnets requires transforming the data to a granular substrate. We present a method using the recently introduced homogeneity order parameter Lambda [H. Agrawal, Phys. Rev. Lett. 89, 268702 (2002)]] for optimizing the parameter controlling the "granularity" and thus the stability of partitions. The model substrates obtained for gene expression data have a highly granular structure. We explore properties of phase transition in high q ferromagnetic Potts models on these substrates and show that the maximum of the width of superparamagnetic domain, corresponding to maximally stable partitions, coincides with the minimum of Lambda.
Immersive Learning Experiences for Surgical Procedures. (2016)

This paper introduces a computer-based system that is designed to record a surgical procedure with multiple depth cameras and reconstruct in three dimensions the dynamic geometry of the actions and events that occur during the procedure. The resulting 3D-plus-time data takes the form of dynamic, textured geometry and can be immersively examined at a later time; equipped with a Virtual Reality headset such as Oculus Rift DK2, a user can walk around the reconstruction of the procedure room while controlling playback of the recorded surgical procedure with simple VCR-like controls (play, pause, rewind, fast forward). The reconstruction can be annotated in space and time to provide more information of the scene to users. We expect such a system to be useful in applications such as training of medical students and nurses.
Early Stage Carcinoma of the Uterine Cervix Effects of Intracavitary Radium Treatment on Lymphoid Cells in Blood and Pelvic Lymph Nodes (1986)

Sixteen patients with early stage carcinoma of the uterine cervix treated with primary radical hysterectomy and pelvic lymphadenectomy were compared with 17 patients who four to six weeks before the operation received intracavitary treatment with radium. The calculated radiation dose to the pelvic wall was approximately 10 Gy. The distribution of lymphoid cells in blood and pelvic lymph nodes was studied by an indirect immunofluorescence technique using monoclonal antibodies. The radium treated group showed a significant reduction of circulating OKT4+ (T helper) and OKT8+ (T suppressor/cytotoxic) lymphocytes. The number of Leu7+ (natural killer) cells and 1D5+ cells (monocytes) was not changed, but the ratio between monocytes and T cells was increased after radium therapy. In cell suspensions obtained from the pelvic lymph nodes, the radium treatment induced a significant reduction of the OKT4+ cell fraction. It is concluded that this low dose rate regimen of intracavitary treatment induces changes in the immune system which are of the same type as those seen after external field irradiation.
Exploiting cross-channel quantizer error correlation in time-interleaved analog-to-digital converters (2011)

Uniform quantizers are often modeled as additive uncorrelated noise sources. This paper explores the validity of the additive noise model in the environment of time-interleaved A/D converters. Cross-channel quantizer error correlation is an important discrepancy that arises for channel time delays in close proximity. It is demonstrated through simulation that negative error correlation occurs for different granularity quantizers in close proximity. Statistical analysis is presented to characterize error correlation between quantizers with different granularity. A technique exploiting this correlation often yields significant performance gains above the optimal additive noise model solution.
Mitigation of chromosome loss in clinical CRISPR-Cas9-engineered T cells (2023)

CRISPR-Cas9 genome editing has enabled advanced T cell therapies, but occasional loss of the targeted chromosome remains a safety concern. To investigate whether Cas9-induced chromosome loss is a universal phenomenon and evaluate its clinical significance, we conducted a systematic analysis in primary human T cells. Arrayed and pooled CRISPR screens revealed that chromosome loss was generalizable across the genome and resulted in partial and entire loss of the chromosome, including in pre-clinical chimeric antigen receptor T cells. T cells with chromosome loss persisted for weeks in culture, implying the potential to interfere with clinical use. A modified cell manufacturing process, employed in our first-in-human clinical trial of Cas9-engineered T cells, <sup>1</sup> dramatically reduced chromosome loss while largely preserving genome editing efficacy. Expression of p53 correlated with protection from chromosome loss observed in this protocol, suggesting both a mechanism and strategy for T cell engineering that mitigates this genotoxicity in the clinic.
Clinical application and validation of an iterative forward projection matching algorithm for permanent brachytherapy seed localization from conebeam‐CT x‐ray projections (2010)

The IFPM algorithm avoids the need to match corresponding seeds in each projection as required by standard back-projection methods. The authors' results demonstrate approximately 1 mm accuracy in reconstructing the 3D positions of brachytherapy seeds from the measured 2D projections. This algorithm also successfully localizes overlapping clustered and highly migrated seeds in the implant.
Complex cancer genomes: Finding signals in the noise (2004)

Human cancers progress through accumulation of a remarkable number of genomic and epigenomic abnormalities. In some tumors, thousands of genes may be deregulated by these abnormalities. Moreover, the spectrum of abnormalities varies considerably between tumors-even those with similar clinical characteristics. This heterogeneity frustrates attempts to identify therapeutic points of attack and causes substantial heterogeneity in individual response. Fortunately, efficient, large-scale genomic analysis tools and biological resources that allow high-resolution genomic assessment of sufficiently large numbers of tumors are now available. This is significant in that recurrent abnormalities can be recognized and assessed biologically. This presentation will focus on these technologies and on lessons learned from their application to human breast and ovarian cancers.
A Theory of Timestamp-Based Concurrency Control for Nested Transactions (1988)

We present a rigorous framework for analyzing timestampbased concurrency control and recovery algorithms for nested transactions. We define a local correctness property, local static atomic@, that affords useful modularity. We show that local static atomicity of each object is sufficient to ensure global serializability. We present generalizations of algorithms due to Reed and Herlihy, and show that each ensures local static atomicity.
Figure 1S from Results of the ADAPT Phase 3 Study of Rocapuldencel-T in Combination with Sunitinib as First-Line Therapy in Patients with Metastatic Renal Cell Carcinoma (2023)

&lt;p&gt;Consort diagram&lt;/p&gt;
Strains across the Acetabular Labrum during Hip Motion (2011)

Although this study does not include muscular forces across the hip joint, it does provide a clue as to the stresses about the labrum through the complete range of motions of the hip, which may help in providing a better understanding of the cause of labral tears and in the protection of labral repairs.
An M-sequence reflection measurement system (2017)

For the realization of an m-sequence-based reflectometer, a correlator performing a pulse compression is necessary in order to determine the impulse response function (IRF) of a device under test (DUT). Using a hardware correlator, a time delayed version of the m-sequence transmit signal is required. In this contribution, a concept for the generation of two m-sequences with a fast and precisely adjustable time lag between them is presented. Programmable linear feedback shift registers (LFSRs) are used for generating the m-sequences. The desired time lag is realized by using different initial states of the LFSRs. To guarantee that the time lag is set within one clock period, a select pulse generator (SPG) has been designed and implemented into the system. The concept has been validated by means of measurements using a realized demonstrator setup. According results show the fast time lag adjustment. Furthermore, reflection measurements have been performed with different coupler architectures and a coaxial cable with a length of 20m. Results obtained with two different couplers are compared regarding the achievable dynamic range.
All-optical bistable band-edge Bloch modes in a two-dimensional photonic crystal (2006)

We demonstrate experimentally all-optical bistable operation in an InP-based two-dimensional photonic crystal slab lying on top of a Bragg reflector. Bistability is obtained around 1550nm, using a low group velocity mode at the band edge of the photonic dispersion characteristic. The origin of the bistable regime is shown to be the fast (275ps relaxation time), electronically induced nonlinear refraction index. A low intensity threshold of 4kW∕cm2 was observed, with a contrast of 65% between the high and low reflectivity states.
Development of a biohybrid lung - unaffected gastransfer of Poly-4-Methyl-1-Pentene gasexchange membranes by endothelialisation (2011)

Objectives: The use of extracorporeal membrane oxygenators is limited by adverse reactions like the activation of the coagulation cascade leading to an insufficient gasexchange. Endothelialization is an important tool to optimize the blood compatibility of artificial materials Aiming towards the development of a bioartificial lung, the effect by endothelialisation of poly-4-methyl-1-pentene gasexchange membranes (PMP) respecting the gasexchange capability was evaluated.
Transient Nonlinear Refraction of Organic Solvents in Liquid and Gas Phase (2016)

The nonlinear refractive transients of organic solvents are measured. The response functions derived from liquids are used to predict pulsewidth dependent n2,eff. Comparisons are made between the second hyperpolarizabilities measured in liquid and gas phases.
Hybrid Modeling of the Electrocoalescence Process in Water-in-Oil Emulsions (2018)

A hybrid model based on the population balance approach was developed to represent the electrocoalescence phenomena in water-in-oil emulsions. A semi-empirical aggregation model was used in a population balance equation to simulate the dehydration of two different crude oils. The unknown parameters of the population balance equation were estimated using experimental data. The experiments were performed in a continuous pilot plant used to evaluate the dehydration of crude oil emulsions with an initial 4–10 wt % of water. Different functional forms for the specific rate of coalescence and sink term of the population balance equation were evaluated. The results of the proposed hybrid model were compared to the experimental data and previous empirical models reported in the literature. The results showed good agreement with the experimental data and values obtained by empirical models.
Near-field characterization of photonic crystal components (2005)

In this paper, we have employed a collection scanning near-field optical microscope (SNOM) for imaging of the propagation of light at telecommunication wavelengths along straight and bent photonic crystal (PC) waveguides with Y-splitters, Z-bends and simple couplers that were fabricated on silicon-on-insulator (SOl) wafers