gebhart/patrepeval huggingface dataset:
Long-Term Persistence and Relevant Therapeutic Impact of High-Titer Viral-Neutralizing Antibody in a Convalescent COVID-19 Plasma Super-Donor: A Case Report (2021)

A convalescent, non-severe, patient with COVID-19 was enrolled as a hyper-immune plasma voluntary donor by the Immuno-Hematology and Transfusion Unit of the Regina Elena National Cancer Institute in Rome, under the TSUNAMI national study criteria. During a nearly 6-month period (May-October 2020), the patient was closely monitored and underwent four hyperimmune plasma collections. Serum SARS-CoV-2 (anti-S + anti-N) IgG and IgM, anti-S1 IgA, and neutralizing titers (NTs) were measured. Anti-SARS-CoV-2 antibody levels steadily decreased. No correlation was found between anti-S/anti-N IgG and IgM levels and viral NT, measured by either a microneutralization test or the surrogate RBD/ACE2-binding inhibition test. Conversely, NTs directly correlated with anti-S1 IgA levels. Hyperimmune donor plasma, administered to five SARS-CoV-2 patients with persistent, severe COVID-19 symptoms, induced short-term clinical and pathological improvement. Reported data suggest that high NTs can persist longer than expected, thus widening hyperimmune plasma source, availability, and potential use. <i>In vitro</i> RBD/ACE2-binding inhibition test is confirmed as a convenient surrogate index for neutralizing activity and patients' follow-up, suitable for clinical settings where biosafety level 3 facilities are not available. IgA levels may correlate with serum neutralizing activity and represent a further independent index for patient evaluation.
An early transition state for folding of the P4-P6 RNA domain (2001)

Tertiary folding of the 160-nt P4-P6 domain of the Tetrahymena group I intron RNA involves burying of substantial surface area, providing a model for the folding of other large RNA domains involved in catalysis. Stopped-flow fluorescence was used to monitor the Mg2+-induced tertiary folding of pyrene-labeled P4-P6. At 35 degrees C with [Mg2+] approximately 10 mM, P4-P6 folds on the tens of milliseconds timescale with k(obs) = 15-31 s(-1). From these values, an activation free energy deltaG(double dagger) of approximately 8-16 kcal/mol is calculated, where the large range for deltaG(double dagger) arises from uncertainty in the pre-exponential factor relating k(obs) and delta G(double dagger). The folding rates of six mutant P4-P6 RNAs were measured and found to be similar to that of the wild-type RNA, in spite of significant thermodynamic destabilization or stabilization. The ratios of the kinetic and thermodynamic free energy changes phi = delta deltaG(double dagger)/delta deltaG(o') are approximately 0, implying a folding transition state in which most of the native-state tertiary contacts are not yet formed (an early folding transition state). The k(obs) depends on the Mg2+ concentration, and the initial slope of k(obs) versus [Mg2+] suggests that only approximately 1 Mg2+ ion is bound in the rate-limiting folding step. This is consistent with an early folding transition state, because folded P4-P6 binds many Mg2+ ions. The observation of a substantial deltaG(double dagger) despite an early folding transition state suggests that a simple two-state folding diagram for Mg2+-induced P4-P6 folding is incomplete. Our kinetic data are some of the first to provide quantitative values for an activation barrier and location of a transition state for tertiary folding of an RNA domain.
High extinction-ratio characteristics over 60 db Mach-Zehnder modulator with asymmetric power splitting y-branches on x-cut Ti:LiNbO 3 (2013)

We investigated a Mach-Zehnder modulator with asymmetric power splitting Y-branches. Output characteristics of a modulator depend on the relation between the power splitting ratios of the front and back Y-branches. By tuning the power splitting ratio of the front Y-branch, we achieved maximum power output operation and high extinction-ratio operation respectively.
Vinculin phosphorylation and barrier failure of coronary endothelial monolayers under energy depletion (1997)

We studied the hypothesis that, in energy-depleted endothelial cells, Ca(2+)-dependent activation of protein kinase C (PKC) causes phosphorylation of vinculin and that this effect is involved in the early loss of endothelial barrier function. Vinculin localization and phosphorylation, PKC activity, and albumin permeability were studied in cultured coronary endothelial monolayers from rats. Ten minutes after the onset of metabolic inhibition by 5 mM potassium cyanide and 5 mM 2-deoxy-D-glucose, immunofluorescence of vinculin at cell-to-cell and cell-to-matrix contacts faded, whereas total cellular vinculin content remained unchanged. During the same time period, vinculin phosphorylation at tyrosine and serine sites increased by 3.9- and 3.5-fold, respectively. Vinculin phosphorylation was related to activation of PKC and an unidentified tyrosine kinase and was elicited by a rise in cytosolic Ca2+ within energy-depleted endothelial cells. Conditions inhibiting vinculin phosphorylation also reduced monolayer permeability induced by energy depletion. These data indicate that vinculin phosphorylation is involved in the progression of hyperpermeability during energy depletion in coronary endothelial monolayers.
Bioreactors and Bioprocessing for Tissue Engineering (2002)

Bioreactor design in tissue engineering is complex, and at the early stages of its development. Design of biologically effective, yet scalable, devices requires intimate collaboration between engineers and biologists to ensure that all aspects are considered fully. Growth conditions, harvesting time, scale-up, storage, and sterility issues all need to be considered and incorporated into the design of bioreactors. Each tissue-engineered product will likely require individualized bioreactor design. However, without a comprehensive understanding of each of these components, bioreactor design and tissue growth to manufacture product will remain at a relatively rudimentary and limited level. Increased fundamental understanding of the issues can have a dramatic impact on the ability to generate tissue-engineered product safely, economically, and in the numbers that are required to fully address the patient populations in need.
Advanced Stimulated Scattering Measurements in Supercritical Fluids (2002)

Abstract : Stimulated Brillouin and Rayleigh scattering was applied to measurements of supercritical fluids. Measurements were performed using an injection-locked Nd:YAG laser as a pump laser and an external cavity diode laser as a probe laser. Improvements were made in stability of the probe laser, allowing more reliable operation and in the stability of the pump laser, allowing narrower bandwidth operation. Improvements were made to a supercritical cell to allow measurements in the near critical region% Software was written to enable automated analysis of measured spectra to extract the widths, shifts, and heights of the electrostrictive Brillouin, thermal Brillouin, and thermal Rayleigh peaks. Comparison of these features with theory has showed consistency of the theory through the relationships between the heights and widths of the thermal Brillouin and thermal Rayleigh peaks. Elastic and thermal properties extracted from the measurements agree with literature values for room temperature and atmospheric pressure conditions. Brillouin and Rayleigh measurements were performed over a wide range of supercritical and near-critical conditions. Interesting, unexpected structure in the behavior of the Brillouin shifts, width, and heights was observed in the supercritical region.
Solar Cells: PtCoFe Nanowire Cathodes Boost Short‐Circuit Currents of Ru(II)‐Based Dye‐Sensitized Solar Cells to a Power Conversion Efficiency of 12.29% (Adv. Funct. Mater. 3/2018) (2018)

In article number 1703282, Shang-Wei Chou, Yun Chi, Hung-Lung Chou, Pi-Tai Chou, and co-workers describe PtCoFe nanowires with rich {111} facets exhibiting superior I−3 reduction activity in DSSCs (dye-sensitized solar cells). The PRT-22 DSSC using Pt49Co23Fe28 nanowire as cathode shows a certified power conversion efficiency of 12.0%, which surpasses the previous power conversion efficiency (PCE) record of the DSSCs using Ru(II)-based dyes.
Antibody orientation at bacterial surfaces is related to invasive infection (2012)

Several of the most significant bacterial pathogens in humans, including Streptococcus pyogenes, express surface proteins that bind IgG antibodies via their fragment crystallizable (Fc) region, and the dogma is that this protects the bacteria against phagocytic killing in blood. However, analysis of samples from a patient with invasive S. pyogenes infection revealed dramatic differences in the presence and orientation of IgG antibodies at the surface of bacteria from different sites. In the throat, IgG was mostly bound to the bacterial surface via Fc, whereas in the blood IgG was mostly bound via fragment antigen-binding (Fab). In infected and necrotic tissue, the Fc-binding proteins were removed from the bacterial surface. Further investigation showed that efficient bacterial IgGFc-binding occurs only in IgG-poor environments, such as saliva. As a consequence, the bacteria are protected against phagocytic killing, whereas in blood plasma where the concentration of IgG is high, the antibodies preferentially bind via Fab, facilitating opsonization and bacterial killing. IgG-poor environments represent the natural habitat for IgGFc-binding bacteria, and IgGFc-binding proteins may have evolved to execute their function in such environments. The lack of protection in plasma also helps to explain why cases of severe invasive infections with IgGFc-binding bacteria are so rare compared with superficial and uncomplicated infections.
Peridynamics molecular dynamics and classical (nonlinear) elasticity. (2016)

Abstract not provided.
Esmolol for Treatment of Intraoperative Tachycardia and/or Hypertension in Patients Having Cardiac Operations. Bolus Loading Technique (1991)

REVES, J. G.; CROUGHWELL, N. D.; HAWKINS, E.; SMITH, L. R.; JACOBS, J. R.; RANKIN, S.; LOWE, J.; TRIGHT, P. VAN Author Information
Purification and Characterization of Hyaluronidase fromStreptococcus dysgalactiae (1989)

Streptococcus dysgalactiae IID 678, belonging to group C of the streptococci, secreted a large amount of hyaluronidase (hyaluronate lyase, EC 4.2.2.1) into a culture medium containing hyaluronic acid. The purification procedures of hyaluronidase were 70% ammonium sulfate precipitation, ECTEOLA-cellulose chromatography, phospho-cellulose chromatography, and gel filtration on Sephacryl S-300. The hyaluronidase was purified approximately 27,000-fold from the culture filtrate. The purified enzyme was homogeneous by SDS-poIyacrylamide gel electrophoresis. The enzyme degradated only hyaluronic acid and chondroitin to zl 4,5-unsaturated disaccharides and did not act on other glycosaminoglycans containing sulfate groups, while the degradation rate of chondroitin was about 1/60 of that of hyaluronic acid. The optimum pH was wide, from pH 5.8 to pH 6.6, and the optimum temperature was 37°C. Fe2 +, Cu2 +, Pb2 +, and Hg2 + ions inhibited the activity strongly and Zn2+ inhibited it by half. The molecular weight of the enzyme was estimated to be 125,000 by gel filtration and 117,000 by SDS-polyacrylamide gel electrophoresis. The enzyme was different immunochemically from the hyaluronidase from Streptococcus pyogenes belonging to group A.
Precoder Design for K-User Interference Channels with Finite Alphabet Signals (2013)

We consider a MIMO K-user interference channel with finite alphabet signaling, and design an optimal precoder for maximizing the sum mutual information between all transmitter-receiver pairs. Perfect channel knowledge is assumed to be available at all the transmitters, but, decoding of the interfering signals is not done at any receiver. A gradient descent algorithm is employed for maximizing the sum mutual information. Simulation results for the three user interference channel show that when compared to conventional schemes which maximize the signal-to-interference plus noise ratio or minimize the mean squared error, the proposed design achieves a significantly better bit error rate performance. Along with the proposed precoder, the minimum distance detector which decodes the desired signal (while treating interference as colored Gaussian noise with a known covariance) was utilized at all the receivers, in order to get this enhanced performance.
YANG Data Model for Generic Operations, Administration, and Maintenance (OAM) (2014)

This document presents YANG Data model for OAM. It provides a
protocol-independent and technology-independent abstraction of key OAM
constructs. These abstractions span OAM configuration and operational
data; they promote uniformity between OAM technologies and support
nested OAM workflows (i.e., performing OAM functions at different
layers through a unified interface).
Nonmyeloablative Blood Stem Cell Transplantation as Adoptive Allogeneic Immunotherapy for Metastatic Renal Cell Carcinoma (2001)

Allogeneic stem cell transplantation has emerged as a potentially curative form of immunotherapy for patients with hematological malignancies that are resistant to conventional chemo/radiotherapy. Donor T cell populations targeting allogeneic minor histocompatibility antigens expressed on the patient's malignant cells are felt to be the driving force of the graft-versus-leukemia reaction, although to date only a handful of these antigens have been fully characterized. Recent data from experimental animal models and limited clinical data in humans suggest that graft-versus-tumor effects, analogous to the graft-versus-leukemia reaction, may be generated against malignancies of epithelial origin. This article reviews the results of a pilot trial demonstrating graft-versus-renal cell carcinoma effects following nonmyeloablative stem cell transplantation, highlighting the potential of allogeneic immunotherapy for treating cancer.
Odd‐skipped related 1 is a novel tumour suppressor gene and a potential prognostic biomarker in gastric cancer (2014)

We report that the odd-skipped related 1 (OSR1) gene encoding a zinc-finger transcription factor was preferentially methylated in gastric cancer by genome-wide methylation screening. OSR1 expression was frequently silenced or down-regulated in gastric cancer cell lines. OSR1 expression was also significantly down-regulated at both mRNA and protein levels in primary gastric cancer tissues compared with adjacent normal tissues. The silencing or down-regulation of OSR1 was closely associated with promoter hypermethylation. Overexpression of OSR1 significantly inhibited cell growth, arrested the cell cycle, and induced apoptosis in the gastric cancer cell lines AGS, MKN28, and MGC803. Conversely, knockdown of OSR1 by OSR1-short hairpin RNA significantly enhanced cell growth, promoted the cell cycle, and inhibited apoptosis in the normal gastric epithelial cell line GES1. The dual-luciferase reporter assay revealed that OSR1 activated p53 transcription and repressed the T-cell factor (TCF)/lymphoid enhancer factor (LEF). Complementary DNA expression array and western blotting showed that OSR1 increased the expression of nuclear p53, p21, Fas, and death receptor-5, and suppressed the expression of cyclin D1 and cyclin-dependent kinase 4 in the p53 signalling pathway. In addition, OSR1 suppressed the expression of cytoplasmic β-catenin, TCF-1, and LEF1 in the Wnt/β-catenin signalling pathway. OSR1 methylation was detected in 51.8% of primary gastric cancer patients (85 of 164) by bisulphite genomic sequencing. Multivariate Cox regression analysis showed that OSR1 methylation was an independent predictor of poor survival. Kaplan-Meier survival curves revealed that OSR1 methylation was associated with shortened survival in TNM stage I-III patients. In conclusion, OSR1 acts as a functional tumour suppressor through the transcriptional activation of p53 and repression of TCF/LEF in gastric cancer. Detection of OSR1 methylation may serve as a potential biomarker of the early stage of gastric cancer.
Analytical Methods for Characterizing Magnetic Resonance Probes (2012)

The efficiency of Gd(III) contrast agents in magnetic resonance image enhancement is governed by a set of tunable structural parameters. Understanding and measuring these parameters requires specific analytical techniques. This Feature describes strategies to optimize each of the critical Gd(III) relaxation parameters for molecular imaging applications and the methods employed for their evaluation.
INTERPLANT AND INTRAPLANT COMPETITION EFFECTS ON MAIN STEM YIELD OF THREE DIVERSE-TILLERING SPRING WHEATS (1990)

Three spring wheat (Triticum aestivum L.) genotypes (Neepawa, Siete Cerros and M1417) were subjected to four levels of tiller removal (MS remaining, MS + T1 remaining, MS + T1 + T2 remaining, control) at three plant densities (two, four and eight plants per 15-cm-diameter pot) in a controlled environment to assess the effects of interplant and intraplant competition on stem dimensions and yield-related traits of genotypes with diverse tillering habits. Both types of competition resulted in reduced kernel weight and main stem grain yield and increased yield per pot. Interplant competition also reduced harvest indices while intraplant competition reduced main stem length and main stem diameter. The oligoculm genotype, M1417, was more sensitive to interplant competition than Siete Cerros and Neepawa. The increased sensitivity of M1417 appears to result from plant mortality combined with the inability to maintain kernel mass under severe interplant competition.Key words: Triticum aestivum, wheat (spring), tiller removal, grain yield, plant competition.
The 2-Norbornyl Cation via the Fragmentations of exo- and endo-2-Norbornyloxychlorocarbenes:  Distinction without Much Difference (2001)

exo- and endo-2-norbornyloxychlorocarbenes (7) were generated photochemically from the corresponding diazirines (6). Both carbenes fragmented to [2-norbornyl cation (carbon monoxide) chloride] ion pairs in MeCN or 1,2-dichloroethane solutions. Products included exo-norbornyl chloride (8), endo-norbornyl chloride (9), norbornene (10), and nortricyclene (11). Fragmentation activation energies were very low (< approximately 4 kcal/mol) and, as a result, the (laser flash photolytic) rate constants for fragmentation were essentially identical for exo-7 and endo-7 ( approximately 5 x 10(5) s(-1) in MeCN). Due to chloride return within the ion pairs, product distributions from exo- and endo-7 differed, with more endo-chloride formed from the endo-carbene: the 8/9 product ratio in MeCN was approximately 41 from exo-7, but only 4.6 from endo-7. Norbornene, formed by proton transfer to Cl(-) within the ion pairs, was a major product in both cases (44% from exo-7 and 62% from endo-7). In MeOH/MeCN, up to 28% of exo-2-norbornyl methyl ether formed at the expense of some of the norbornene, but even in 100% MeOH, the norbornyl chloride products of ion pair return still accounted for 46% and 31% of the exo-7 and endo-7 product mixtures (accompanied by 26-32% of norbornene). Electronic structure calculations on the ground states and fragmentation transition states of exo-7 and endo-7 are presented.
1801 Flow Analysis of a Fan Wake in a Rectangular Channel using IB Method (2011)

Numerical solving of flow around the complex -shape body is of importance in various sci - entific elds But there are problems in that t takes too much computational time fbr the interpolation scheme between the body and computational grid In the present paper we propose the novel numerical scheme by using
Operating experience with ABB Power Plant Laboratories multi-use combustion test facility (1999)

Combustion Engineering, Inc.'s ABB Power Plant Laboratories (PPL) has installed a new Multi-Use Combustion Test Facility to support the product development needs for ABB Group's Power Generation Businesses. This facility provides the flexibility to perform testing under fluidized bed combustion, conventional pulverized-coal firing, and gasification firing conditions, thus addressing the requirements for several test facilities. Initial operation of the facility began in late 1997. This paper will focus on the design and application of this Multi-Use Combustion Test Facility for fluidized bed product development. In addition, this paper will present experimental facility results from initial circulating fluidized bed operation, including combustion and environmental performance, heat transfer, and combustor profiles.
Oxytocin antagonists with changes in the Asn5position shed light on hormone‐oxytocin receptor interactions (1991)

Since oxytocin agonists and antagonists have different structure-activity relationships, we have investigated the stereostructural and stereoelectronic requirements of the Asn5 residue in oxytocin antagonists by the synthesis of four analogues of the potent, prolonged acting oxytocin antagonist [Pen1,D-Phe2,Thr4,Orn8]-oxytocin (I) in which Asn5 was replaced respectively with Thr (II), Leu5 (III), Asp5 (IV) and Tyr5 (V). These analogues had pA2 values in the antioxytocic in vitro rat uterine assay of 7.23 (I), 7.16 (II), 6.67 (III), 7.21 (IV), and 6.76 (IV), respectively. All were also found to be weakly potent in the in vivo anti-vasopressor assay in the rat. These studies demonstrate very different structural and stereoelectronic requirements for oxytocin agonists and antagonists when they interact with the oxytocin uterine receptor.
In-Situ Focusing Inside a Thermal Vacuum Chamber (2010)

Traditionally, infrared (IR) space instruments have been focused by iterating with a number of different thickness shim rings in a thermal vacuum chamber until the focus meets requirements. This has required a number of thermal cycles that are very expensive as they tie up many integration and test (I&T)/ environmental technicians/engi neers work ing three shifts for weeks. Rather than creating a test shim for each iteration, this innovation replaces the test shim and can focus the instrument while in the thermal vacuum chamber. The focus tool consists of three small, piezo-actuated motors that drive two sets of mechanical interface flanges between the instrument optics and the focal- plane assembly, and three optical-displacement metrology sensors that can be read from outside the thermal vacuum chamber. The motors are used to drive the focal planes to different focal distances and acquire images, from which it is possible to determine the best focus. At the best focus position, the three optical displacement metrology sensors are used to determine the shim thickness needed. After the instrument leaves the thermal vacuum chamber, the focus tool is replaced with the precision-ground shim ring. The focus tool consists of two sets of collars, one that mounts to the backside of the interface flange of the instrument optics, and one that mounts to the backside of the interface flange of the focal plane modules. The collars on the instrument optics side have the three small piezo-actuated motors and the three optical displacement metrology systems. Before the instrument is focused, there is no shim ring in place and, therefore, no fasteners holding the focal plane modules to the cameras. Two focus tooling collars are held together by three strong springs. The Orbiting Carbon Observatory (OCO) mission spectrometer was focused this way (see figure). The motor described here had to be moved five times to reach an acceptable focus, all during the same thermal cycle, which was verified using pupil slicing techniques. A focus accuracy of .20.100 microns was achieved.