gebhart/patrepeval huggingface dataset:
Regulation of eukaryotic DNA replication at the initiation step (2003)

The studies of cell growth and division have remained at the centre of biomedical research for more than 100 years. The combination of genetic, biochemical, molecular and cell biological techniques recently yielded a burst in what is known of the molecular control of cell growth processes. The initiation of DNA replication is crucial for the stability of the genetic information of a cell. Two factors, Cdc45p (cell division cycle 45p) and DNA polymerase alpha-primase, are necessary in this process. Depending on growth signals, Cdc45p is expressed as a late protein. New phosphorylation-specific antibodies specifically recognize the phosphorylated subunit, p68, of the four subunit DNA polymerase alpha-primase and show that the phosphorylated polypeptide is exclusively nuclear.
A hybrid numerical/analytic technique for the computation of wave fields in stratified media based on the Hankel transform (1984)

A hybrid numerical/analytic technique is presented for computing the field due to a monochromatic point source in a horizontally stratified medium. It is based on the exact Hankel transform relationship between the field in the range domain and the associated depth-dependent Green’s function in the horizontal wavenumber domain. The method uses a numerical evaluation of the Hankel transform. It is shown that a major source of error in such an evaluation arises from undersampling of the Green’s function at points where it becomes infinite. This error is described in terms of aliasing, analogous to the aliasing that has been well-described for the discrete Fourier transform. It is shown that the error can be substantially reduced by removing the infinities, calculating the Hankel transform of the remaining portion of the Green’s function numerically, and adding to it the analytically computed Hankel transform of the infinities. The sum of the analytic terms and the remaining Hankel transform always exactly equals the true field with no errors introduced other than those associated with the numerical evaluation of the Hankel transform, and the method is accurate in both the near- and farfield regions. The technique is developed in detail for the acoustics problem of a monochromatic point source and receiver in an isovelocity fluid half-space overlying a horizontally stratified fluid medium. It is found that under circumstances of interest in ocean bottom acoustics, where the Green’s function has only a few singularities along the real horizontal wavenumber axis, the technique is efficient and extremely accurate.
Biological determinants of photobioreactor design. Third quarterly report, March 1, 1994--May 31, 1994 (1994)

PBR system with an external hollow fiber gas exchanger is optimized to have a higher ratio of illuminated volume to dark volume and an optimum oxygen production rate, future application of the algal PBR to regenerate the atmosphere in space may be possible.
Highly Efficient Organic Light‐Emitting Devices Prepared with a Phosphorescent Heteroleptic Iridium (III) Complex Containing 7,8‐Benzoquinoline as the Cyclometalated Ligand (2014)

The performance of a heteroleptic bis‐cyclometalated complex based on 7,8‐benzoquinoline ligands is investigated as a new emitter in phosphorescent OLEDs. The devices show very high efficiency at various dopant concentrations and maximum efficiency is achieved at 8% dopant concentration. A comparison of the performance of PHOLEDs with the new emitter versus those with Ir(ppy)3 emitter reveals an improvement of efficiency at all dopant concentrations. These preliminary results obtained with the first bzq‐based emitter indicate that this family of cyclometalated proligands is a promising compound for the development of highly emissive complexes.
Traffic-controlled rate monotonic priority scheduling of ATM cells (2002)

Future (broadband) integrated services networks are expected to carry various types of traffic including real-time packet video for which stringent quality of service (QoS) requirements must be met. We propose a cell multiplexing scheme for providing real-time communication services in point-to-point ATM networks. Our scheme is simple enough to be used in high-speed ATM networks, yet reasonably efficient in terms of accommodating channel requests. The scheme employs the leaky bucket model as the input traffic description model and regulates the input traffic at user network interface (UNI) to comply with the input traffic specification. Inside the network ATM switches provide bounded local delays to individual cells using a traffic controller and a non-preemptive rate-monotonic priority scheduler.
A new mechanism for the selectivity to C1 and C2 species in the electrochemical reduction of carbon dioxide on copper electrodes (2011)

We have investigated the reaction mechanism of the electrochemical reduction of carbon dioxide to hydrocarbons on copper electrodes. This reaction occurs via two pathways: a C1 pathway leading to methane, and a C2 pathway leading to ethylene. To identify possible intermediates in the reduction of carbon dioxide we have studied the reduction of small C1 and C2 organic molecules containing oxygen. We followed the formation and consumption of intermediates during the reaction as a function of potential, using online mass spectrometry. For the C1 pathway we show that it is very likely that CHOads is the key intermediate towards the breaking of the C–O bond and, therefore, the formation of methane. For the C2 pathway we suggest that the first step is the formation of a CO dimer, followed by the formation of a surface-bonded enediol or enediolate, or the formation of an oxametallacycle. Both the enediol(ate) and the oxametallacycle would explain the selectivity of the C2 pathway towards ethylene. This new mechanism is significantly different from existing mechanisms but it is the most consistent with the available experimental data.
CBIO-19. GENOME SHREDDING ENABLES CRISPR-MEDIATED GLIOBLASTOMA ONCOLYSIS (2021)

Abstract Glioblastoma (GBM) is the most common and lethal primary brain tumor in adults 1. Despite multimodal treatment regimens including surgical resection, radio- and chemotherapy, the growth of residual tumor often results in therapy resistance and ultimately death. GBMs are highly diffuse and exhibit extensive intratumoral heterogeneity 2,3, confounding diagnostic efforts and presenting opportunities for therapy evasion. Therefore, innovative treatment paradigms that can efficiently eliminate GBM cells irrespective of their mutational and epigenetic profile are urgently needed. CRISPR technologies have revolutionized medicine by enabling targeted genome editing through RNA-guided introduction of DNA double-strand breaks 4,5. Here, we show that CRISPR-Cas9 mediated genome fragmentation through targeting of highly repetitive loci, termed “genome shredding”, enables rapid and robust elimination of GBM cells. We characterized genome shredding across mammalian and vertebrate cells, and identified optimal repetitive pan-vertebrate and species-specific loci. Genome shredding is equally effective in temozolomide (TMZ)-sensitive and -resistant GBM cells, and multi-cycle treatment regimens are feasible. Importantly, when deployed in intracerebral GBM xenografts through local delivery, CRISPR-Cas9 genome shredding efficiently eliminated all targeted cells. Together, genome shredding enables the rapid and efficient fragmentation of a target cell’s genome and subsequent DNA damage-induced cell death. This provides an innovative treatment paradigm that is independent of a tumor’s mutational and epigenetic profile and leverages CRISPR-Cas9 as a breakthrough therapeutic modality for GBM.
[Possible involvement of a recently discovered human retrovirus in idiopathic immunologic disorders, including Sjögren syndrome (autoimmune exocrinopathy)]. (1993)

Recent studies have suggested that newly-discovered human retroviruses may contribute to the pathogenesis of several immunological diseases. We linked a human intracisternal A-type retroviral particle (HIAP) to systemic autoimmune diseases, such as Sjögren's Syndrome. A-type retroviruses are envelope-deficient, a property that may contribute to their pathobiology and epidemiology. Potential mechanisms by which a defective retrovirus could induce immune dysfunctions are discussed.
Effect of Optical Delay on the Suppression of the Power Transient Excursion in a Combined Gain-Controlled Erbium-Doped Fiber Amplifier (2003)

This report describes the effect of optical delay on the suppression of the power transient excursion in a combined gain-controlled erbium-doped fiber amplifier with an internal optical feedback loop (OFL). A simple homogeneous model showed that the optical delay caused a phase change in the oscillation of the surviving and laser channels, which resulted in a reduction of the overall power transient excursion. In addition to the reduction, a real system with a 1528.7-nm OFL shifted the oscillation upward or downward according to channel removal or addition, whereas another one with a 1560.9-nm OFL did not. This different transient behavior reflected a control-wavelength dependence on optical automatic gain control, where spectral-hole burning dominated over relaxation oscillation for 1528.7 nm, and vice versa for 1560.9 nm.
Modelling 6 MV photon beams of a stereotactic radiosurgery system for Monte Carlo treatment planning (2004)

The goal of this work is to build a multiple source model to represent the 6 MV photon beams from a Cyberknife stereotactic radiosurgery system for Monte Carlo treatment planning dose calculations. To achieve this goal, the 6 MV photon beams have been characterized and modelled using the EGS4/BEAM Monte Carlo system. A dual source model has been used to reconstruct the particle phase space at a plane immediately above the secondary collimator. The proposed model consists of two circular planar sources for the primary photons and the scattered photons, respectively. The dose contribution of the contaminant electrons was found to be in the order of 10(-3) of the total maximum dose and therefore has been omitted in the source model. Various comparisons have been made to verify the dual source model against the full phase space simulated using the EGS4/BEAM system. The agreement in percent depth dose (PDD) curves and dose profiles between the phase space and the source model was generally within 2%/1 mm for various collimators (5 to 60 mm in diameter) at 80 to 100 cm source-to-surface distances (SSD). Excellent agreement (within 1%/1 mm) was also found between the dose distributions in heterogeneous lung and bone geometry calculated using the original phase space and those calculated using the source model. These results demonstrated the accuracy of the dual source model for Monte Carlo treatment planning dose calculations for the Cyberknife system.
Sequences complementary to the brain-specific “identifier” sequences exist in L-type pyruvate kinase mRNA (a liver-specific messenger) and in transcripts especially abundant in muscle. (1986)

A sequence complementary to the brain-specific identifier sequence has been found in the 3' untranslated extension of the heavy 3.2-kilobase (kb) long liver L-type pyruvate kinase mRNA while it is absent in the other two 2- and 2.2-kb long pyruvate kinase mRNA species. A 53-base fragment corresponding to this identifier sequence was subcloned in both orientations in the single-stranded bacteriophage M13, both strands being used as probes to detect homologous sequence in different tissues. Both strands are transcribed in various tissues and are detected in heterogeneous high molecular weight RNA species which are especially abundant in the adult muscle. In addition, the probe identical to the identifier sequence recognized a discrete 0.6-kb RNA species in the muscle and the probe complementary to the identifier-sequence recognized the expected two small brain-specific identifier BC-1 and BC-2 RNAs described by Sutcliffe et al. (Sutcliffe, J. G., Milner, R. J., Bloom, F. E., and Lerner, R. A. (1982) Proc. Natl. Acad. Sci. U. S. A. 79, 4942-4946; Sutcliffe, J. G., Milner, R. J., Gottesfeld, J. M., and Lerner, R. A. (1984) Nature 308, 237-241; Milner, R. J., Bloom, F. E., Lai, C., Lerner, R. A., and Sutcliffe, J. G. (1984) Proc. Natl. Acad. Sci. U. S. A. 81, 713-717; Sutcliffe, J. G., Milner, R. J., Gottesfeld, J. M., and Reynolds, W. (1984) Science 225, 1308-1314).
Potential of ultra-high voltage silicon carbide semiconductor devices (2016)

In this paper, the theoretical performance of ultra-high voltage Silicon Carbide (SiC) based devices are investigated. The SiC semiconductor device conduction power loss and switching power loss are predicted and compared with different modeling approaches, for SiC metal-oxide semiconductor field-effect transistors (MOSFETs) up to 20 kV and SiC gate turn-off (GTO) thyristors and SiC insulated-gate bipolar transistors (IGBTs) up to 50 kV. A parameter sensitivity analysis has been performed to observe the device power loss under various operating conditions, for instance current density, temperature and charge carrier lifetime. Also, the maximum allowed current density and maximum switching frequency for a maximum chip power dissipation limit of 300 W/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> are investigated. The simulation results indicate that the SiC MOSFET has the highest current capability up to approximately 15 kV, while the SiC IGBT is suitable in the range of 15 kV to 35 kV, and thereafter the SiC GTO thyristor supersedes the loss performance from 35 kV to 50 kV.
ChemInform Abstract: AMP Deaminase Inhibitors. Part 3. SAR of 3‐(Carboxyarylalkyl)coformycin Aglycon Analogues. (2000)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Quantification of adult T-cell leukemia/lymphoma cells using simple four-color flow cytometry (2014)

Our newly established method quantified tumor cells in patients with acute-type ATL. Furthermore, this method was useful for assessing the efficacy of chemotherapy, and the change of the CD7N proportion could be more important to predict prognosis.
157-nm chromeless phase lithography for 45-nm SRAM gate (2004)

157-nm lithography processes together with optimization of mask feature size and illumination conditions and chromeless mask (CLM) of mesa-type were used to fabricate a 45-nm gate by combining a high numerical aperture (NA) lens with off-axis illumination (OAI) and using Si-containing resist. It was observed that the minimum pitch for forming a 45-nm line was 140-nm. It was also shown that quadrupole illumination was the optimum OAI condition and the optimum mask feature size for forming a 45-nm line of 200-nm pitch was between 50 nm to 55 nm. In these conditions the normalized image log-slope value was about 3.0. It was demonstrated that a 45-nm SRAM gate with a depth of focus of 150 nm could be fabricated by combining these resolution enhancement techniques with high NA lithography and Si-containing resist. Furthermore the 45-nm SRAM-gate pattern was successfully transferred with a bi-layer process. From these results it was proven that fabrication of 45-nm node device could be achieved by using CLM with high NA lithography.
NOVEL MULTIWAVELENGTH MODE-LOCKED SEMICONDUCTOR LASERS: PHYSICS AND APPLICATIONS (2000)

We develop and study the physics of a novel hybrid wavelength-division-multiplexed, optical timedivision-multiplexed (WDM-OTDM) mode-locked semiconductor laser for applications in ultra-high data rate communication links, computer interconnects, and optical sampling applications. The key philosophy behind using a hybrid approach is that state-of-the-art system performance can be achieved without the necessity of operating at the limits of a either a pure WDM or OTDM technology platform.
Induction of cholinergic differentiation with neurite sprouting by de novo biosynthesis and expression of GD3 and b-series gangliosides in Neuro2a cells. (1994)

The expression of a single glycosyltransferase, GD3 synthase, caused cholinergic differentiation with neurite sprouting. The cells that expressed GD3 were established from Neuro2a cells by transfection of a mammalian expression vector into which were carried a cDNA encoding GD3 synthase and the blasticidin-S-deaminase gene with a SV40 promoter, followed by selection with blasticidin-S-hydrochloride. The blasticidin-S-hydrochloride-resistant colonies derived from the cells transfected with the cDNA encoding GD3 synthase and the clonal cells (N2a-GD3) were spontaneously sprouting neurites but not those derived from cells transfected with only the vector without the cDNA encoding GD3 synthase (N2a-bsr). GD3 expression by N2a-GD3 was confirmed by immunostaining of the cells using the anti-GD3 monoclonal antibody, KM643. N2a-GD3 expressed not only GD3 but also GQ1b, one of the b-series gangliosides, whereas N2a-bsr did not express these gangliosides. Cell proliferation of N2a-GD3 was greatly reduced, as compared with that of N2a-bsr, and, after several passages, it completely stopped. In addition, N2a-GD3 expressed acetylcholine esterase, indicating that the differentiation of Neuro2a cells was induced by expression of GD3 synthase and subsequent modification of the biosynthesis and expression of gangliosides. These results strongly suggest that the de novo synthesis and expression of GD3 and/or b-series gangliosides induce neurite outgrowth and differentiation of Neuro2a cells. Exogenous GM1 stimulated the neuritogenesis of N2a-bsr but not differentiated N2a-GD3, indicating that the mechanism of neurite sprouting in this system may be overlapped en route with that of exogenous GM1.
Hydroxypropyl Methylcellulose-Based Hydrogel Copolymeric for Controlled Delivery of Galantamine Hydrobromide in Dementia (2020)

The study aims to prepare a smart copolymeric for controlled delivery of Galantamine hydrobromide. The synthesis of the hydrogel was executed through free radical polymerization using HPMC (Hydroxypropyl methylcellulose) and pectin as polymers and acrylic acid as monomer. Cross-linking was performed by methylene bisacrylamide (MBA). HPMC-pectin-co-acrylic acid hydrogel was loaded with Galantamine hydrobromide (antidementia drug) as a model drug for treatment of Alzheimer based dementia. Formulated hydrogels (SN1–SN9) were characterized for Fourier transform-infrared spectroscopy, differential scanning calorimetry, thermogravimetric analysis, X-ray diffraction, and energy dispersive X-ray. Drug loading efficiency, gel fraction, measurements of porosity, and tensile strength were reported. Swelling and release studies were performed at pH 1.2 and 7.4. Drug liberation mechanism was evaluated by applying different release kinetic models. Galantamine hydrobromide was released from prepared hydrogels by Fickian release mechanism. Swelling, gel fraction, porosity, and drug release percentages were found to be dependent on hydroxypropyl methylcellulose, pectin, acrylic acid, and methylene bisacrylamide concentrations. By increasing HPMC amount, swelling was increased from 76.7% to 95.9%. Toxicity studies were conducted on albino male rabbits for a period of 14 days. Hematological and histopathological studies were carried out to evaluate safety level of hydrogel. Successfully prepared HPMC-pectin-co-acrylic acid hydrogel showed good swelling and release kinetics, which may help greatly in providing controlled release drug effect leading to enhanced patient compliance for dementia patients.
Minimally invasive lateral extracavitary corpectomy: cadaveric evaluation model and report of 3 clinical cases (2012)

A minimally invasive lateral extracavitary thoracic corpectomy has the ability to provided excellent spinal cord decompression and VB resection. The procedure can be completed safely and successfully with minimal blood loss and little associated morbidity. This approach has the potential to improve upon established traditional open corridors for posterolateral thoracic corpectomy.
Overexpression of SK2 Channels Enhances Efferent Suppression of Cochlear Responses Without Enhancing Noise Resistance (2007)

Cochlear hair cells express SK2, a small-conductance Ca(2+)-activated K(+) channel thought to act in concert with Ca(2+)-permeable nicotinic acetylcholine receptors (nAChRs) alpha9 and alpha10 in mediating suppressive effects of the olivocochlear efferent innervation. To probe the in vivo role of SK2 channels in hearing, we examined gene expression, cochlear function, efferent suppression, and noise vulnerability in mice overexpressing SK2 channels. Cochlear thresholds, as measured by auditory brain stem responses and otoacoustic emissions, were normal in overexpressers as was overall cochlear morphology and the size, number, and distribution of efferent terminals on outer hair cells. Cochlear expression levels of SK2 channels were elevated eightfold without striking changes in other SK channels or in the alpha9/alpha10 nAChRs. Shock-evoked efferent suppression of cochlear responses was significantly enhanced in overexpresser mice as seen previously in alpha9 overexpresser mice; however, in contrast to alpha9 overexpressers, SK2 overexpressers were not protected from acoustic injury. Results suggest that efferent-mediated cochlear protection is mediated by other downstream effects of ACh-mediated Ca(2+) entry different from those involving SK2-mediated hyperpolarization and the associated reduction in outer hair cell electromotility.
On tuning leakage for performance-robust adaptive control (1989)

The behavior of a model reference adaptive control (MRAC) system is examined. The effect of the inclusion of leakage in the adaptive algorithm is studied, and the leakage mechanism of the sigma -modification and the e/sub 1/-modification are discussed. A useful approximation is given for a particular MRAC problem for the small step-size average parameter estimate motion away from an arbitrary stabilizing adaptive controller parameterization. Arrow diagrams verify the conclusion that designer-selected leakage constants must be well chosen in relation to the unknown plant and environment to avoid bursting by an adaptive controller in the absence of adequate excitation by the external forcing function(s).< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Modeling and filtering athetoid movement for assistive computer interfaces (2008)

Numerous anticounterfeiting platforms using photoresponsive materials have been designed to improve information security, enabling applications in anticounterfeiting technology. However, fabricating sophisticated micro/nanostructures using bidirectional mass transport to achieve advanced anticounterfeiting remains challenging. Here, we propose one strategy to achieve steerable mass transport in a photoresponsive system with the assistance of solvent vapor at room temperature. Upon optimizing the host-guest ratio and the width of photoisomerized areas, wettability gradient is acquired just photo-patterning once, then bidirectional mass transport is realized due to the competition of mass transport induced by surface energy gradient of the material itself and flow of the solvent on the film surface with wettability gradient. Taking advantage of the interaction between solvent and film surface with wettability gradient, this bidirectional polymer flow has been successfully applied in multi-mode anticounterfeiting. This work paves a promising avenue toward high-level information storage in soft materials, demonstrating the potential applications in anticounterfeiting.