gebhart/patrepeval huggingface dataset:
A high-throughput SAMDI-mass spectrometry assay for isocitrate dehydrogenase 1 (2020)

The enzyme isocitrate dehydrogenase 1 (IDH1) catalyzes the conversion of isocitrate to alpha-ketoglutarate (αKG) and has emerged as an important therapeutic target for glioblastoma multiforme (GBM). Current methods for assaying IDH1 remain poorly suited for high-throughput screening of IDH1 antagonists. This paper describes a high-throughput and quantitative assay for IDH1 that is based on the self-assembled monolayers for matrix-assisted laser desorption/ionization-mass spectrometry (SAMDI-MS) method. The assay uses a self-assembled monolayer presenting a hydrazide group that covalently captures the αKG product of IDH1, where it can then be detected by MALDI-TOF mass spectrometry. Co-capture of an isotopically-labeled αKG internal standard allows the αKG concentration to be quantitated. The assay was used to analyze a series of standard αKG solutions and produced minimal error in measured αKG concentration values. The suitability of the assay for high-throughput analysis was evaluated in a 384-sample biochemical IDH1 screen. Cells expressing IDH1 were lysed and the lysate was applied to the monolayer to capture αKG, which was then quantitated using the SAMDI-MS assay. Cells in which IDH1 expression was reduced by small-interfering RNA exhibited a corresponding decrease in αKG concentration as measured by the assay. Application of the assay toward the high-throughput screening of IDH1 inhibitors or knockdown agents may facilitate the discovery of treatments for GBM.
Prediction of Osmotic and Activity Coefficients Using a Modified Pitzer Equation for Multicomponent Strong Electrolyte Systems at 298 K (2003)

We have predicted the osmotic and activity coefficients of strong electrolyte solutions using a modification of the Pitzer equation. The modified equation can be used for multicomponent aqueous solutions by applying a mixing rule at the Debye−Hückel term. We have found that the modification of the Pitzer equation retains the accuracy of the original equation without using any characteristic parameters evaluated from the experimental data. The new equation is predictive and simpler than the original Pitzer equation.
5.1: Invited Paper : A 5.7″ Color Mirasol® XGA Display For High Performance Applications (2011)

Abstract A 5.7″ color reflective display module based on bi‐stable Interference Modulation Devices (IMODs) with a projective capacitive touchscreen is described. The operation of the module is outlined and the performance reported. The system level developments necessary to achieve the high reflectivity, color and video operation while maintaining low power consumption are discussed.
Improved dielectric mirrors for the THz frequency range (2006)

Dielectric mirrors are widely used in optical setups for spectral regions such as UV, visible, as well as IR. Yet, for the rapidly growing field of terahertz spectroscopy dielectric multilayer optics are sparsely utilized. But with low-loss materials high quality THz optics can be obtained. We present two approaches for the realization of highly effective dielectric THz mirrors. First, four thin slices of high-resistivity silicon and five common polypropylene (PP) foils were alternately stacked together to obtain a broad reflection band. This stop-band blueshifts with increasing angles of incidence. But due to the high index step between Si and PP a band from 0.32 to 0.375 THz always remains the stopband for all incidence angles and both the s- and p-polarization. The measurement data obtained in reflection and transmission geometry are reproduced well by numerical simulations. With a minor change of the layer sequence a microresonator is obtained which reveals a sharp transmission peak at around 0.3 THz within the reflection band. The second material system consists of ceramic laminates of alumina (A) and alumina-zirconia (AZ). Measurements on 12.5 pairs of A/AZ layers yield a strong stop-band from 0.3 to 0.37 THz at normal incidence, which again match numerical simulations. The big advantage of the ceramic mirror is the rugged, quasimonolithic design of the sintered multilayer structure.
ChemInform Abstract: Photoluminescence and ODMR Studies of Lamellar Cd2P2S6 and Zn2P2S6 Lattices. (1994)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Regulation of N‐methyl‐D‐aspartate receptors revealed by intracellular dialysis of murine neurones in culture. (1989)

1. The whole-cell patch clamp recording technique was employed to investigate the intracellular regulation of N-methyl-D-aspartate (NMDA) receptors in cultured murine hippocampal neurones. Excitatory amino acids were repeatedly applied at regular intervals during intracellular dialysis with solutions of various composition. 2. Currents evoked by L-aspartate, an agonist of NMDA receptors, gradually 'washed out' to approximately 50% of their initial amplitude during dialysis with an intracellular solution containing CsCl and EGTA as a calcium buffer. In contrast, responses to kainate did not wash out. The wash-out of L-aspartate currents followed an exponential time course with a time constant of about 150 s. Wash-out did not appear to be related to desensitization of NMDA receptors. 3. Following wash-out, L-aspartate responses were blocked by Mg2+, ketamine or D-2-amino-5-phosphonovalerate indicating that these responses were still mediated by NMDA receptors. Furthermore, responses to NMDA itself showed wash-out to the same extent and with a time course similar to that for L-aspartate responses. 4. Neither the time course nor the extent of the wash-out of responses to L-aspartate was affected when the Ca2+ concentration of the dialysate was varied from zero to 1.5 x 10(-5) M. In addition, wash-out was unaffected by substitution of BAPTA for EGTA, indicating that wash-out was not a consequence of changes in intracellular pH related to the binding of Ca2+ to the buffer or to the kinetics of this binding. Therefore, the wash-out of NMDA currents could not be attributed to a gradual elevation of the concentration of intracellular Ca2+. 5. The extent of the wash-out of L-aspartate currents was similar for cells held at +40 versus -60 mV although the rate of wash-out was slower at the depolarized potential. In addition, the reversal potential of these currents was not altered, demonstrating that a change in driving force did not account for a component of the wash-out. 6. Inclusion of an ATP regeneration solution (Forscher & Oxford, 1985) in the dialysate prevented the wash-out of L-aspartate currents. ATP alone was less effective in preventing wash-out whereas phosphocreatine and creatine phosphokinase were ineffective by themselves. Wash-out also occurred when ATP was replaced with the non-hydrolysable analogue, beta, gamma-methyleneATP, or with GTP. In cells where wash-out of L-aspartate currents had been established, subsequent dialysis with the ATP regenerating solution partially reversed this wash-out.(ABSTRACT TRUNCATED AT 400 WORDS)
Synthesis, Equilibration, and Coupling of 4-Lithio-1,3-dioxanes:  Synthons for syn- and anti-1,3-Diols. (1999)

ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionNEXTORIGINAL ARTICLEThis notice is a correctionSynthesis, Equilibration, and Coupling of 4-Lithio-1,3-dioxanes: Synthons for syn- and anti-1,3-Diols.Scott D. Rychnovsky, Alexandre J. Buckmelter, Vilas H. Dahanukar, and Donald J. SkalitzkyCite this: J. Org. Chem. 1999, 64, 20, 7678Publication Date (Web):September 8, 1999Publication History Published online8 September 1999Published inissue 1 October 1999https://pubs.acs.org/doi/10.1021/jo9949927https://doi.org/10.1021/jo9949927correctionACS PublicationsCopyright © 1999 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views279Altmetric-Citations3LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (65 KB) Get e-Alertsclose Get e-Alerts
The mid-IR silicon photonics sensor platform (Conference Presentation) (2017)

Advances in integrated silicon photonics are enabling highly connected sensor networks that offer sensitivity, selectivity and pattern recognition. Cost, performance and the evolution path of the so-called ‘Internet of Things’ will gate the proliferation of these networks. The wavelength spectral range of 3-8um, commonly known as the mid-IR, is critical to specificity for sensors that identify materials by detection of local vibrational modes, reflectivity and thermal emission. For ubiquitous sensing applications in this regime, the sensors must move from premium to commodity level manufacturing volumes and cost. Scaling performance/cost is critically dependent on establishing a minimum set of platform attributes for point, wearable, and physical sensing. Optical sensors are ideal for non-invasive applications. Optical sensor device physics involves evanescent or intra-cavity structures for applied to concentration, interrogation and photo-catalysis functions. The ultimate utility of a platform is dependent on sample delivery/presentation modalities; system reset, recalibration and maintenance capabilities; and sensitivity and selectivity performance. The attributes and performance of a unified Glass-on-Silicon platform has shown good prospects for heterogeneous integration on materials and devices using a low cost process flow. Integrated, single mode, silicon photonic platforms offer significant performance and cost advantages, but they require discovery and qualification of new materials and process integration schemes for the mid-IR. Waveguide integrated light sources based on rare earth dopants and Ge-pumped frequency combs have promise. Optical resonators and waveguide spirals can enhance sensitivity. PbTe materials are among the best choices for a standard, waveguide integrated photodetector. Chalcogenide glasses are capable of transmitting mid-IR signals with high transparency. Integrated sensor case studies of i) high sensitivity analyte detection in solution; ii) gas sensing in air and iii) on-chip spectrometry provide good insight into the tradeoffs being made en route to ubiquitous sensor deployment in an Internet of Things.
Research highlights: micro-engineered therapies (2014)

Lab on a chip systems have often focused on diagnostic, chemical, and cell analysis applications, however, more recently the scale and/or precision of micro-engineered systems has been applied in developing new therapies. In this issue we highlight recent work using microfluidic and micro-engineered systems in therapeutic applications. We discuss two approaches that use microfluidic precision to address challenges in filtering blood--to both remove unwanted pathogens and toxins and isolate rare cells of interest that have therapeutic potential. In both cases chemically-modified surfaces, a bioengineered mannose binding lectin on magnetic particles and antibody-functionalized reversibly degradable alginate film, provide the functionality to remove (or isolate) target cells of interest. The third paper we highlight generates microscale gels as protective niches for cell-based therapies. Importantly, the microgels are designed to have controlled porosity but also mechanical rigidity to protect housed therapeutic cells, like mesenchymal stem cells. We expect continued progress in micro- & nano-enabled therapies facilitated by the fabrication of new microstructured materials, precise separations, and closed-loop sensing and drug delivery.
Investigation of the Coupled Effect of Liquid and Solid Inorganic Additives on Thermal Stability and Water Retention of PFSA Composite (2018)

Here, investigation of the high-temperature water retention property of new composites, which contain perfluorosulfonic acid (PFSA) polymer as matrix, a mineral acid, and MgF2 nanoparticles as inorganic additives, is done based on structural analysis. The composite shows an improvement in the water removal temperature from 50 to 250 °C because the crystalline water molecules were attached on the surface of solid inorganic additives at 250 °C. To optimize the water retention property, the 1 wt % of sulfuric acid and 3–15 wt % of MgF2-coated glass particles in PFSA polymer composites as additives were investigated. From the Fourier transform infrared-attenuated total reflection studies of these composite membranes, two major phenomena related to structural details of PFSA polymer as a function of the polymer and the weight ratio of the solid additives are observed: (1) sulfonic acid group released the proton even with low humidity and (2) the conformation and/or the degree of the crystallinity of the poly(tetrafluoroethylene) hydrophilic domains change in the membrane. It is found that the sulfuric acid connects the sulfonic acid groups of the polymer and MgF2 nanoparticles, coated on the surface of acidic glass particles, by direct bonding among them.
Synthesis of Z-(Pinacolato)allylboron and Z-(Pinacolato)alkenylboron Compounds through Stereoselective Catalytic Cross-Metathesis (2013)

The first examples of catalytic cross-metathesis (CM) reactions that furnish Z-(pinacolato)allylboron and Z-(pinacolato)alkenylboron compounds are disclosed. Products are generated with high Z selectivity by the use of a W-based monoaryloxide pyrrolide (MAP) complex (up to 91% yield and >98:2 Z:E). The more sterically demanding Z-alkenylboron species are obtained in the presence of Mo-based MAP complexes in up to 93% yield and 97% Z selectivity. Z-selective CM with 1,3-dienes and aryl olefins are reported for the first time. The utility of the approach, in combination with catalytic cross coupling, is demonstrated by a concise and stereoselective synthesis of anticancer agent combretastatin A-4.
Analysis of free space optical (FSO) communications and networking using adaptive waveforms for LEO satellites (2020)

In this paper, we model the use of free space optical (FSO) links that employ adaptive waveforms such as n-PSK in the context of links to and from low earth orbit (LEO) satellite systems. FSO link models are described that can be used to predict dynamic link quality to and from a surface terminal to a LEO terminal. Because the link margin can vary by 10-20 dB in a LEO pass, we consider the use of adaptive waveforms. Higher order waveforms are used when the link margin is high, stepping back down to on-off keying when the margin is low. We also describe the design and development of network emulation and simulation capabilities to help analyze the use of dynamic FSO-LEO link models within existing network experimentation frameworks. We present a network emulation demonstration of the designed FSO-LEO link models involving data transfer between two surface observers and multiple opportunistic LEO satellite events. We discuss both the emerging opportunities and ongoing challenges of effectively using such FSO-LEO capabilities within a larger data network context.
73.1: Large‐Area Plasma‐Panel Radiation Detectors for Nuclear Medicine Imaging to Homeland Security and the Super Large Hadron Collider (2010)

Abstract A new radiation sensor derived from plasma panel display technology is introduced. It has the capability to detect ionizing and non‐ionizing radiation over a wide energy range and the potential for use in many applications. The principle of operation is described and some early results presented.
MIMO throughput measurement in an urban area using a LTE mobile terminal (2012)

TEST 02 - Elsevier's Scopus, the largest abstract and citation database of peer-reviewed literature. Search and access research from the science, technology, medicine, social sciences and arts and humanities fields.
Profiling the Protection of Sensitive Enterprise Multimedia Communication (2009)

Voice is, besides email, the major personal communication technology used by employees of an enterprise. Additionally, multimedia communication is getting used more and more in the form of video conferences to decrease travel cost and support environmental protection. Besides oral communication also documents, e.g. presentations, may be shared. Thus sensitive information is likely to be exchanged as part of the communication. Hence, an appropriate security level is of great relevance to protect enterprisepsilas confidential information. Historically, security measures for communication systems have either been targeted to prevent fraud, thereby securing the operatorpsilas business model, or to provide military-class end-to-end encryption. Neither approach targets the security objectives of enterprises using private and public communication infrastructure for sensitive business exchanges. It is common practice within enterprises to define different data protection classes and associated rules that have to be respected when dealing with sensitive information. Thus, the main contribution here is on one hand an evaluation of relevant available or known security technology from the perspective of protecting sensitive enterprise communication, and a roadmap for an incremental, step-wise introduction of security features in multimedia (including voice and video) communication that allows for a step-wise (incremental) introduction of security features required for different security levels.
Correction: A highly selective dual-therapeutic nanosystem for simultaneous anticancer and antiangiogenesis therapy (2020)

Correction for ‘A highly selective dual-therapeutic nanosystem for simultaneous anticancer and antiangiogenesis therapy’ by Lizhen He <italic>et al.</italic>, <italic>J. Mater. Chem. B</italic>, 2017, <bold>5</bold>, 8228–8237, DOI: 10.1039/C7TB02163A.
Acinetobacter nosocomialis Causes as Severe Disease as Acinetobacter baumannii in Northeast Thailand: Underestimated Role of A. nosocomialis in Infection (2022)

Infections by Acinetobacter species are recognized as a serious global threat due to causing severe disease and their high levels of antibiotic resistance. Acinetobacter baumannii is the most prevalent pathogen in the genus, but infection by Acinetobacter nosocomialis has been reported widely. Diagnosis of patients with A. baumannii infection is often misdiagnosed with other Acinetobacter species, especially A. nosocomialis. This study investigated whether there were significant differences in clinical outcomes between patients infected with A. baumannii versus A. nosocomialis in Northeast Thailand, and to characterize serological responses to infection with these pathogens. The results show that A. baumannii had higher levels of multidrug resistance. Despite this, clinical outcomes for infection with A. baumannii or A. nosocomialis were similar with mortalities of 33% and 36%, respectively. Both pathogens caused community-acquired infections (A. baumannii 35% and A. nosocomialis 29% of cases). Plasma from uninfected healthy controls contained IgG antibody that recognized both organisms, and infected patients did not show a significantly enhanced antibody response from the first week versus 2 weeks later. Finally, the patterns of antigen recognition for plasma IgG were similar for patients infected with A. baumannii or A. nosocomialis infection, and distinct to the pattern for patients infected with non-Acinetobacter. In conclusion, our data revealed that infection with A. nosocomialis was associated with a similarly high level of mortality as infection with A. baumannii, the high rate of community-acquired infection and antibodies in uninfected individuals suggesting that there is significant community exposure to both pathogens. <b>IMPORTANCE</b> Bacterial infections by Acinetobacter species are global threats due to their severity and high levels of antibiotic resistance. A. baumannii is the most common pathogen in the genus; however, infection by A. nosocomialis has also been widely reported but is thought to be less severe. In this study, we have prospectively investigated 48 reported cases of A. baumannii infection in Northeast Thailand, and characterized the serological responses to infection. We found that 14 (29%) of these infections were actually caused by A. nosocomialis. Furthermore, the incidence of antibiotic resistance among A. nosocomialis strains, APACHE II scores, and mortality for patients infected with A. nosocomialis were much higher than published data. Both A. baumannii and A. nosocomialis had unexpectedly mortality rates of over 30%, and both pathogens caused a high rate of community-acquired infections. Importantly, background antibodies in uninfected individuals suggest significant community exposure to both pathogens in the environment.
Mitochondrial DNA copy number is proportional to total cell DNA under a variety of growth conditions. (1990)

Analyses of populations of NIH/3T3 cells for mitochondrial DNA under various conditions were made by use of an improved procedure. Cells were examined at different cell densities, at different stages of cell growth, and after the cells had been incubated with a carcinogen reported to affect mitochondrial DNA replication, benzo[a]pyrene-epoxide. In addition, the analysis was performed on three tumorigenic NIH/3T3 cell lines and one mouse bladder epithelial cell line and its tumorigenic derivative. Small variations of mitochondrial DNA under these various conditions were detected easily and revealed a linear relationship between mitochondrial DNA and total cell DNA.
Navi-Ball: A New Guidance Device for CT-Directed Punctures (2001)

This "low-tech" system offers easy to use guidance by a single operator and provides for adequate accuracy of first-time needle placement.