gebhart/patrepeval huggingface dataset:
Adenylate Kinase 1 Deficiency Induces Molecular and Structural Adaptations to Support Muscle Energy Metabolism (2003)

Genetic ablation of adenylate kinase 1 (AK1), a member of the AK family of phosphotransfer enzymes, disturbs muscle energetic economy and decreases tolerance to metabolic stress, despite rearrangements in alternative high energy phosphoryl transfer pathways. To define the mechanisms of this adaptive response, soleus and gastrocnemius muscles from AK1(-/-) mice were characterized by cDNA array profiling, Western blot and ultrastructural analysis. We demonstrate that AK1 deficiency induces fiber-type specific variation in groups of transcripts involved in glycolysis and mitochondrial metabolism and in gene products defining structural and myogenic events. This was associated with increased phosphotransfer capacities of the glycolytic enzymes pyruvate kinase and 3-phosphoglycerate kinase. Moreover, in AK1(-/-) mice, fast-twitch gastrocnemius, but not slow-twitch soleus, had an increase in adenine nucleotide translocator (ANT) and mitochondrial creatine kinase protein, along with a doubling of the intermyofibrillar mitochondrial volume. These results provide molecular evidence for wide-scale remodeling in AK1-deficient muscles aimed at preservation of efficient energetic communication between ATP producing and utilizing cellular sites.
A 12-Gb/s 11-mW half-rate sampled 5-tap decision feedback equalizer with current-integrating summers in 45-nm SOI CMOS technology (2008)

The design and experimental results of a low-power, low-area 5-tap DFE implemented in 45-nm SOI CMOS technology are reported. The DFE employs a low-power current-integrating summer with sampling front-end, which eliminates systematic frequency-dependent loss inherent in conventional integrating serial receivers. Further power and area savings are achieved through the use of a direct-feedback architecture and CMOS-style rail-to-rail clocking. The 5-tap DFE core occupies 73 times 50 mum <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> and consumes 11 mW from a 1V supply when equalizing 12-Gb/s data passed over a 30rdquo channel with 15 dB of loss at 6 GHz.
A Simple, General, and GPU Friendly Method for Computing Dual Mesh and Iso-Surfaces of Adaptive Mesh Refinement (AMR) Data (2020)

We propose a novel approach to extracting crack-free iso-surfaces from Structured AMR data that is more general than previous techniques, is trivially simple to implement, requires no information other than the list of AMR cells, and works, in particular, for different AMR formats including octree AMR, block-structured AMR with arbitrary level differences at level boundaries, and AMR data that consist of individual cells without any existing grid structure. We describe both the technique itself and a CUDA-based GPU implementation of this technique, and evaluate it on several non-trivial AMR data sets.
Harmonic Power Harvesting System for Passive RFID Sensor Tags (2016)

This paper introduces a relevant concept of energy harvesting for passive UHF radio frequency identification (RFID) relying on the exploitation of the power carried by the third harmonic signal generated by the RFID chip. The idea consists on the use of the sole third harmonic energy to power up an associated sensor to the RFID tag. The proposed concept is first demonstrated in simulation thanks to an equivalent model for the RF front-end of a passive UHF RFID chip. Although the proposed model is simplified, it considers the generated nonlinear signals, allowing an efficient design of the rectifier circuit, which is responsible of harvesting the third harmonic power besides ensuring the activation of the RFID chip in order to communicate with the reader. The power driving between the RFID chip, third harmonic harvester, and antenna at the fundamental and third harmonic frequencies is achieved by designing a low-loss distributed three-port impedance matching network. Simulation results confirm the operation of the matching network and the exploitation of the third harmonic signal by analyzing the power at different nodes in the circuit. Measurement results validate the proposed nonlinear chip model. A prototype of the RFID tag harmonic-harvester produces 39 μW of dc power harvested from the harmonic signal, showing good agreement with the simulation results. Finally, a sensor application exploiting the harmonic harvested power to energize a commercial temperature sensor at a distance of 80 cm from the reader is demonstrated.
Image-degradation in a two-wave mixing spatial-light modulator (1991)

Optical real-time image processing by spatial light modulators (SLM’s) that utilize photorefractive crystals has attracted much attention /1/. The two-wave mixing SLM is based on the selective spatial erasure of the uniform phase volume hologram created by the two plane coherent beams using an incoherent image bearing beam I inc (r→) resulting in an additional spatial modulation of the refractive index distribution I 1 (r→) (commonly with a greater spatial period then the grating period of the hologram). The parameters of these Bragg-type SLM’s are strongly determined by the diffraction properties of the nonuniform volume gratings and are analyzed here by means of the two-wave mixing coupled-wave formalism in it’s two-dimensional description /2/.
Application of the magnetic ear for flux balancing of a 160kW/20kHz DC-DC converter transformer (2013)

The non-ideal behavior of semiconductor devices used in power electronic circuits exciting a transformer can cause DC voltage components applied to the transformer terminals. This DC voltage in turn generates a DC current and/or DC flux density component only limited by the parasitic resistance of the windings and semiconductors. The biased flux density operation deteriorates the performance of the converter, since the core can be driven into saturation, generating higher currents and hence higher temperatures in the circuit. In order to overcome this problem, the previously reported “Magnetic Ear”, a non-invasive flux density transducer concept, is used in this paper. The design, placement and all implementation issues for this flux density transducer are described. For verifying the theoretical considerations, the transducer is used to perform a closed loop control over the DC component of the flux density in the 166kW/20kHz transformer, therefore ensuring its unbiased magnetic flux density operation.
1143: Ethnic Background has Minimal Impact on the Etiology of Nephrolithiasis (2004)

You have accessJournal of UrologyDiscussed Poster, Monday, May 10, 2004, 1:00 - 5:00 pm1 Apr 20041143: Ethnic Background has Minimal Impact on the Etiology of Nephrolithiasis W Patrick Springhart, Wesley Ekeruo, Michaella E. Maloney, Chivuzo U. Enemchukwu, Matthew D. Young, Yeh Hong Tan, James O. L'Esperance, David M. Albala, and Glenn M. Preminger W Patrick SpringhartW Patrick Springhart More articles by this author , Wesley EkeruoWesley Ekeruo More articles by this author , Michaella E. MaloneyMichaella E. Maloney More articles by this author , Chivuzo U. EnemchukwuChivuzo U. Enemchukwu More articles by this author , Matthew D. YoungMatthew D. Young More articles by this author , Yeh Hong TanYeh Hong Tan More articles by this author , James O. L'EsperanceJames O. L'Esperance More articles by this author , David M. AlbalaDavid M. Albala More articles by this author , and Glenn M. PremingerGlenn M. Preminger More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(18)38380-0AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "1143: Ethnic Background has Minimal Impact on the Etiology of Nephrolithiasis." The Journal of Urology, 171(4S), pp. 301–302 © 2016 by American Urological AssociationFiguresReferencesRelatedDetails Volume 171Issue 4SApril 2004Page: 301-302 Advertisement Copyright & Permissions© 2016 by American Urological AssociationMetricsAuthor Information W Patrick Springhart More articles by this author Wesley Ekeruo More articles by this author Michaella E. Maloney More articles by this author Chivuzo U. Enemchukwu More articles by this author Matthew D. Young More articles by this author Yeh Hong Tan More articles by this author James O. L'Esperance More articles by this author David M. Albala More articles by this author Glenn M. Preminger More articles by this author Expand All Advertisement Loading ...
Phorbol ester stimulation of RasGRP1 regulates the sodium-chloride cotransporter by a PKC-independent pathway (2007)

The sodium-chloride cotransporter (NCC) is the principal salt-absorptive pathway in the mammalian distal convoluted tubule (DCT) and is the site of action of one of the most effective classes of antihypertensive medications, thiazide diuretics. We developed a cell model system to assess NCC function in a mammalian cell line that natively expresses NCC, the mouse DCT (mDCT) cell line. We used this system to study the complex regulation of NCC by the phorbol ester (PE) 12-O-tetradecanoylphorbol-13-acetate (TPA), a diacylglycerol (DAG) analog. It has generally been thought that PEs mediate their effects on transporters through the activation of PKC. However, there are at least five other DAG/PE targets. Here we describe how one of those alternate targets of DAG/PE effects, Ras guanyl-releasing protein 1 (RasGRP1), mediates the PE-induced suppression of function and the surface expression of NCC. Functional assessment of NCC by using thiazide-sensitive (22)Na(+) uptakes revealed that TPA completely suppresses NCC function. Biotinylation experiments demonstrated that this result was primarily because of decreased surface expression of NCC. Although inhibitors of PKC had no effect on this suppression, MAPK inhibitors completely prevented the TPA effect. RasGRP1 activates the MAPK pathway through activation of the small G protein Ras. Gene silencing of RasGRP1 prevented the PE-mediated suppression of NCC activity, the activation of the H-Ras isoform of Ras, and the activation of ERK1/2 MAPK. This finding confirmed the critical role of RasGRP1 in mediating the PE-induced suppression of NCC activity through the stimulation of the MAPK pathway.
Supplementary Figure 4 from NVP-BEZ235, a Dual PI3K/mTOR Inhibitor, Prevents PI3K Signaling and Inhibits the Growth of Cancer Cells with Activating PI3K Mutations (2023)

Supplementary Figure 4 from NVP-BEZ235, a Dual PI3K/mTOR Inhibitor, Prevents PI3K Signaling and Inhibits the Growth of Cancer Cells with Activating PI3K Mutations
Current Membrane Research Membrane Research: Classic Origins and Current Concepts A. L. Muggleton-Harris (1982)

Journal Article Current Membrane Research Get access Membrane Research: Classic Origins and Current Concepts, edited by Muggleton-Harris A. L.. Academic Press, New York, 1981, 428 p., illus., (74-17773). M. J. Hope, M. J. Hope Biochemistry Department, University of British Columbia, Vancouver, BC, Canada V6T 1W5 Search for other works by this author on: Oxford Academic Google Scholar P. R. Cullis P. R. Cullis Biochemistry Department, University of British Columbia, Vancouver, BC, Canada V6T 1W5 Search for other works by this author on: Oxford Academic Google Scholar BioScience, Volume 32, Issue 10, November 1982, Page 811, https://doi.org/10.2307/1308979 Published: 01 November 1982
Experimental Investigation of Separation Control Part 1: Baseline and Steady Suction (2006)

Low-speed flow separation over a wall-mounted hump, and its control using steady suction, were studied experimentally in order to generate a data set for the development and evaluation of computational methods. The baseline and controlled data sets comprised time-mean and unsteady surface pressure measurements, flowfield measurements using particle image velocimetry, and wall shear stress obtained via oil-film interferometry. In addition to the specific test cases studied, surface pressures for a wide variety of conditions were acquired for different Reynolds numbers and suction rates. Stereoscopic particle image velocimetry and oil-film flow visualization indicated that the baseline time-averaged separated flowfield was two-dimensional. With the application of control, mild three-dimensionality was evident in the spanwise variation of pressure recovery, reattachment location, and spanwise pressure fluctuations.
A Phase II Trial of Vinblastine Plus Dipyridamole in Advanced Renal Cell Carcinoma A Hoosier Oncology Group Study (1994)

One potential explanation for why renal cell carcinoma is usually poorly responsive to chemotherapy is intrinsic multidrug resistance. Dipyridamole (DP) is one of several agents known to bind to P-glycoprotein and potentially reverse multidrug resistance in vitro, and dosages needed to obtain appropriate levels in vivo appear to be well tolerated. The current study was undertaken to evaluate the combination of vinblastine (VLB) and DP in patients with advanced renal cell carcinoma and no prior chemotherapy. From August 1989 through December 1989, 15 patients with inoperable recurrent or metastatic renal cell carcinoma were treated with VLB 0.2 mg/kg (i.v. slow push) and concurrently received DP75 mg p.o. q.i.d. starting 48 hours before and continuing 48 hours after VLB administration. Courses were repeated every 3 weeks for a maximum of 8 weeks, or until disease progression or undue toxicities ensued. The predominant toxicities seen were mild neurotoxicity and leukopenia. Only 1 patient had grade IV leukopenia, and no lethal toxicities occurred. No objective responses were seen; 2 patients had stable disease for 29 and 30+ months. The median survival was 9 months (range: 2.5-30+). We conclude that at the dose and schedule used in this study, the combination of VLB and DP may be administered with acceptable toxicities, but is ineffective in the treatment of advanced renal cell carcinoma.
MobiCom poster (2003)

article Share on MobiCom poster: practical experience with HTTP and TCP over GPRS Authors: Rajiv Chakravorty University of Cambridge Computer Laboratory, Cambridge, U.K. University of Cambridge Computer Laboratory, Cambridge, U.K.View Profile , Ian Pratt University of Cambridge Computer Laboratory, Cambridge, U.K. University of Cambridge Computer Laboratory, Cambridge, U.K.View Profile Authors Info & Claims ACM SIGMOBILE Mobile Computing and Communications ReviewVolume 7Issue 1January 2003 pp 41–43https://doi.org/10.1145/881978.881990Online:01 January 2003Publication History 0citation466DownloadsMetricsTotal Citations0Total Downloads466Last 12 Months0Last 6 weeks0 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my Alerts New Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
SERS Active Surface in Two Steps, Patterning and Metallization (2012)

Abstract Robust and reproducible metallized nano/microstructured surfaces of polymeric surfaces have been successfully prepared by direct laser interference patterning (DLIP) of commercial polymeric films followed by sputtering of metallic thin films. The SERS spectra for 2‐thioaniline adsorbed on a structured polycarbonate surfaces covered with a gold or platinum film showed a ca. three order of magnitude enhancement over a flat surface with the same metal film. The method here reported is suitable for mass production of substrates for SERS since large areas (several cm 2 ) can be structured in ca. 1–5 s.
CMOS Electrochemical Imaging Arrays for the Detection and Classification of Microorganisms (2021)

Microorganisms account for most of the biodiversity on earth. Yet while there are increasingly powerful tools for studying microbial genetic diversity, there are fewer tools for studying microorganisms in their natural environments. In this paper, we present recent advances in CMOS electrochemical imaging arrays for detecting and classifying microorganisms. These microscale sensing platforms can provide non-optical measurements of cell geometries, behaviors, and metabolic markers. We review integrated electronic sensors appropriate for monitoring microbial growth, and present measurements of single-celled algae using a CMOS sensor array with thousands of active pixels. Integrated electrochemical imaging can contribute to improved medical diagnostics and environmental monitoring, as well as discoveries of new microbial populations.
Laser Flash Photolysis in High Speed Photopolymer Coating Layers (1994)

Abstract Sensitization mechanisms of two systems of (dialkylamino)styryl-type dyes with a radical-generating reagent, 2-[p-(diethylamino)styryl]-, and 2-[2-(9-julolidinyl)vinyl]naphtho[1,2-d]thiazole with 3,3′,4,4′-tetrakis(t-butyldioxycarbonyl)benzophenone, in poly(methyl methacrylate) film have been investigated by laser flash photolysis using a total reflection cell.
Transcriptomic basis for an antiserum against Micrurus corallinus (coral snake) venom (2009)

Besides an improvement in our knowledge of the composition of coral snake venoms, which are very poorly known when compared to Old World elapids, the expression profile suggests abundant and diversified components that may be used in future antiserum formulation. As recombinant production of venom antigens frequently fails due to complex disulfide arrangements, DNA immunization may be a viable alternative. In fact, the selected candidates provided an initial evidence of the feasibility of this approach, which is less costly and not dependent on the availability of the venom.
Orientation of Polymer Functionalized Nanorods in Thin Films (2010)

A directed self assembly of anisotropic nanostructures offers a possibility to provide unique functional materials, which are e.g., important in optoelectronic devices. We use the liquid crystalline behavior of polymer functionalized TiO2 and ZnO nanorods to apply methods well known for low molecular liquid crystals to achieve oriented thin films. Convective forces in the meniscus on a structured substrate obtain thin layers of oriented nanoparticles with a ordering parameter of S = 0.7. As another method we present the orientation of polystyrene covered ZnO nanorods under an applied electric field. The method offers a perpendicular alignment of the rods to the surface.
Active thermal control of power electronics modules (2004)

Active thermal control techniques make it feasible to regulate the steady state and transient thermal-mechanical stress in power electronic modules for applications such as motor drives. On-line junction temperature estimation and manipulation of the switching frequency and current limit to regulate the losses, are used to prevent over-temperature and power cycling failures in IGBT power modules. The techniques developed in this work are used to actively control the junction temperature of the power module. This control strategy improves power module reliability and increases utilization of the silicon thermal capacity by providing sustained operation at maximum attainable performance limits.
Modular lipid nanoparticle platform technology for siRNA and lipophilic prodrug delivery (2020)

ABSTRACT Successfully employing therapeutic nucleic acids, such as small interfering RNA (siRNA), requires chemical modifications or the use of nanocarrier technology to prevent their degradation in the circulation and to facilitate intracellular delivery. Lipid nanoparticles (LNP) are among the most advanced nanocarriers culminating in the first siRNA therapeutic’s clinical translation and approval. At the same time, their applicability as modular platform technology due to the interchangeable building blocks and siRNA payload hallmarks one of LNPs’ major advantages. In addition, drug derivatization approaches to synthesize lipophilic small molecule prodrugs enable stable incorporation in LNPs. This provides ample opportunities to develop combination therapies by co-encapsulating multiple therapeutic agents in a single formulation. Here, we describe how the modular LNP platform can be applied for combined gene silencing and chemotherapy to induce additive anti-cancer effects. We show that various lipophilic taxane prodrug derivatives and siRNA against the androgen receptor, a prostate cancer driver, can be efficiently and stably co-encapsulated in LNPs. In addition, we demonstrate that prodrug incorporation does not affect LNPs’ gene silencing ability and that the combination therapy induces additive therapeutic effects in vitro . Using a double-radiolabeling approach, we quantitively determined the LNPs’ and prodrugs’ pharmacokinetic properties and biodistribution following systemic administration in tumor-bearing mice. Our results indicate that co-encapsulation of siRNA and lipophilic prodrugs into LNPs is an attractive and straightforward approach for combination therapy development. GRAPHICAL ABSTRACT
Resistivity and Hall voltage study of phosphorus segregation in polycrystalline Si1−xGex films (2002)

Hall and resistivity measurements as functions of temperature were used to study the segregation of phosphorus in atmospheric-pressure chemical-vapor deposited, ∼300-nm-thick, polycrystalline Si0.95Ge0.5 and Si0.9Ge0.1 thin films, implanted at 80 keV with 6×1013–5×1014 P/cm2, and annealed for 1 h at 800 °C. Hall measurements, feasible only in heavily doped films, indicated that 29% of the dopant in Si0.9Ge0.1 and 42% of the dopant in Si0.95Ge0.05 were electrically inactive. The loss is consistent with dopant segregation to grain boundaries. The density of grain-boundary carrier traps is 3.6×1012/cm2 in Si0.95Ge0.05 and 4.4×1012/cm2 in Si0.9Ge0.1, increasing sublinearly with Ge content.
HLA-A∗02:01 restricted T cell receptors against the highly conserved SARS-CoV-2 polymerase cross-react with human coronaviruses (2021)

Cross-reactivity and direct killing of target cells remain underexplored for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2)-specific CD8<sup>+</sup> T cells. Isolation of T cell receptors (TCRs) and overexpression in allogeneic cells allows for extensive T cell reactivity profiling. We identify SARS-CoV-2 RNA-dependent RNA polymerase (RdRp/NSP12) as highly conserved, likely due to its critical role in the virus life cycle. We perform single-cell TCRαβ sequencing in human leukocyte antigen (HLA)-A<sup>∗</sup>02:01-restricted, RdRp-specific T cells from SARS-CoV-2-unexposed individuals. Human T cells expressing these TCRαβ constructs kill target cell lines engineered to express full-length RdRp. Three TCR constructs recognize homologous epitopes from common cold coronaviruses, indicating CD8<sup>+</sup> T cells can recognize evolutionarily diverse coronaviruses. Analysis of individual TCR clones may help define vaccine epitopes that can induce long-term immunity against SARS-CoV-2 and other coronaviruses.
Positron annihilation in carbon nanotube powders (1999)

Positron lifetime spectra have been measured in two kinds of carbon nanotube powders as a function of temperature range between 32 and 296 K. It has been found that all spectra are essentially temperature-independent in the above temperature range. The results of analysis show that there are three components in the powders of carbon nanotube with an average diameter of 30 nm, and four components in the powders of carbon nanotube with typical diameters of around 15 nm. The average values of lifetime components obtained at various temperatures are about 220, 390 ps, and 2.0 ns for the former, and about 140, 300, 650 ps and 6.4 ns for the latter.