gebhart/patrepeval huggingface dataset:
Synthesis of OligonucleotidePeptide Conjugates Carrying the c‐myc Peptide Epitope as Recognition System (2004)

Oligonucleotide-peptide conjugates 1-3 were prepared by sequential addition of the appropriate Boc-protected amino acids, followed by nucleoside phosphoramidites in the same support. These molecules are designed to be used for triplex formation and for the directed assembly of nanomaterials. The structures of the desired oligonucleotide-peptide conjugates were confirmed by mass spectrometry on small oligonucleotide-peptide conjugates, by gel electrophoresis, and by hybridization with complementary oligonucleotides. Oligonucleotides carrying the c-myc peptide were specifically recognized by the anti-c-myc monoclonal antibody.
Differential Gene Expression by Lactobacillus plantarum WCFS1 in Response to Phenolic Compounds Reveals New Genes Involved in Tannin Degradation (2017)

<i>Lactobacillus plantarum</i> is a lactic acid bacterium that can degrade food tannins by the successive action of tannase and gallate decarboxylase enzymes. In the <i>L. plantarum</i> genome, the gene encoding the catalytic subunit of gallate decarboxylase (<i>lpdC</i>, or <i>lp_2945</i>) is only 6.5 kb distant from the gene encoding inducible tannase (<i>L. plantarum</i><i>tanB</i> [<i>tanB<sub>Lp</sub></i> ], or <i>lp_2956</i>). This genomic context suggests concomitant activity and regulation of both enzymatic activities. Reverse transcription analysis revealed that subunits B (<i>lpdB</i>, or <i>lp_0271</i>) and D (<i>lpdD</i>, or <i>lp_0272</i>) of the gallate decarboxylase are cotranscribed, whereas subunit C (<i>lpdC</i>, or <i>lp_2945</i>) is cotranscribed with a gene encoding a transport protein (<i>gacP</i>, or <i>lp_2943</i>). In contrast, the tannase gene is transcribed as a monocistronic mRNA. Investigation of knockout mutations of genes located in this chromosomal region indicated that only mutants of the gallate decarboxylase (subunits B and C), tannase, GacP transport protein, and TanR transcriptional regulator (<i>lp_2942</i>) genes exhibited altered tannin metabolism. The expression profile of genes involved in tannin metabolism was also analyzed in these mutants in the presence of methyl gallate and gallic acid. It is noteworthy that inactivation of <i>tanR</i> suppresses the induction of all genes overexpressed in the presence of methyl gallate and gallic acid. This transcriptional regulator was also induced in the presence of other phenolic compounds, such as kaempferol and myricetin. This study complements the catalog of <i>L. plantarum</i> expression profiles responsive to phenolic compounds, which enable this bacterium to adapt to a plant food environment.<b>IMPORTANCE</b><i>Lactobacillus plantarum</i> is a bacterial species frequently found in the fermentation of vegetables when tannins are present. <i>L. plantarum</i> strains degrade tannins to the less-toxic pyrogallol by the successive action of tannase and gallate decarboxylase enzymes. The genes encoding these enzymes are located close to each other in the chromosome, suggesting concomitant regulation. Proteins involved in tannin metabolism and regulation, such GacP (gallic acid permease) and TanR (tannin transcriptional regulator), were identified by differential gene expression in knockout mutants with mutations in genes from this region. This study provides insights into the highly coordinated mechanisms that enable <i>L. plantarum</i> to adapt to plant food fermentations.
Abstract 1300: Novel thyrointegrin αvβ3 antagonist in the treatment of glioblastoma multiforme (2019)

Abstract Background: The proliferative and pro-angiogenic actions mediated by L-thyroxine, pro-inflammatory and all known growth factors in glioblastoma multiforme (GBM) are initiated at cell surface thyrointegrin αvβ3 receptors for thyroid hormone on the extracellular domain of integrin αvβ3. Thyrointegrin αvβ3 receptors are over-expressed on cancer and rapidly dividing blood vessel cells, but quiescent on normal cells. A macromolecule Polyethylene glycol-conjugated bi-TriAzole Tetraiodothyroacetic acid (P-bi-TAT) acts with high affinity (Ki 3.1 nM) and specificity for the thyrointegrin αvβ3 receptors without any significant nuclear translocation. Methods: In the present studies, three primary human GBM cells and U87 glioma cell line were implanted orthotopically or subcutaneously (s.c.) into nude mice that were treated daily for up to 21 days with P-bi-TAT at different doses. Additionally, preclinical Pharmacokinetic and Safety assessments were carried out in multiple species. Results: Pharmacokinetic profiles indicated once-a-day dosing, and safety assessment studies demonstrated high safety and tolerability at 100-fold over the anticipated human equivalent dose. Fluorescence labeled P-bi-TAT administered s.c. demonstrated high biodistribution to GBM tumor in the brain that was comparable to peripherally implanted tumor. P-bi-TAT administered daily for 10-21 days s.c. at 0.3-10 mg/kg resulted in a dose-dependent suppression of GBM tumor growth and viability monitored with IVIS imaging (p &amp;lt;0.001). Histopathological analysis of tumors revealed 95% loss of the vascularity of treated tumors at 10 days (p &amp;lt;0.001) along with extensive cellular necrosis and apoptosis, without intratumoral hemorrhage. GBM tumors had a 97% volume loss and maximal loss of GBM cell viability during a subsequent 22 days off-treatment period (p &amp;lt; 0.001). Genomic micro-array studies with human primary GBM revealed that over 30 pathways relevant to the progression of GBM are modulated by P-bi-TAT. Conclusions: P-bi-TAT is a promising lead clinical candidate effective in the treatment of human GBM. Citation Format: Shaker A. Mousa, Thangirala Sudha, Kavitha Godugu, Mehdi Rajabi, Stewart Sell, Paul J. Davis. Novel thyrointegrin αvβ3 antagonist in the treatment of glioblastoma multiforme [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 1300.
NX210c Peptide Promotes Glutamatergic Receptor-Mediated Synaptic Transmission and Signaling in the Mouse Central Nervous System (2022)

NX210c is a disease-modifying dodecapeptide derived from the subcommissural organ-spondin that is under preclinical and clinical development for the treatment of neurological disorders. Here, using whole-cell patch-clamp recordings, we demonstrate that NX210c increased α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR)- and GluN2A-containing N-methyl-D-aspartate receptor (GluN2A-NMDAR)-mediated excitatory postsynaptic currents in the brain. Accordingly, using extracellular field excitatory postsynaptic potential recordings, an enhancement of synaptic transmission was shown in the presence of NX210c in two different neuronal circuits. Furthermore, the modulation of synaptic transmission and GluN2A-NMDAR-driven signaling by NX210c restored memory in mice chronically treated with the NMDAR antagonist phencyclidine. Overall, by promoting glutamatergic receptor-related neurotransmission and signaling, NX210c represents an innovative therapeutic opportunity for patients suffering from CNS disorders, injuries, and states with crippling synaptic dysfunctions.
Coaxing the Maximum Performance out of an Ordinary Structured MO Disk (1996)

An ordinary structured magneto-optical disk was improved and optimized to achieve high areal recording density using the magnetic field modulation recording technique. After studying film material/thickness, groove geometry, and birefringence in a poly-carbonate substrate, a track pitch of 0.85 µm with a linear recording density of 0.31 µm/bit is determined to be a good combination for a wavelength of 690 nm and an objective lens with a numerical aperture of 0.55, which results in an areal density of 3.8 bit/µm 2 or 2.4 Gbit/inch 2 .
SOLUBILITY OF TOCOPHEROL AND TOCOTRIENOLS FROM PALM OIL IN SUPERCRITICAL CARBON DIOXIDE (2007)

ABSTRACT The supercritical fluid extraction of tocols (tocopherol and tocotrienols) from palm oil was carried out in a dynamic (flow‐through) system. The experiments were carried out in a 3 × 3 factorial design with three equally spaced pressures (14, 22 and 30 MPa) and three equally spaced temperatures (40, 60 and 80C). The tocols obtained were quantified using off‐line high‐performance liquid chromatography with fluorescent detection. The effects of operating pressure and temperature on the extraction and solubility of tocols in supercritical carbon dioxide were investigated. The results showed that tocols have solubility in the range of 0.94 × 10 −3 to 0.018 mg/g CO 2 for the conditions investigated in this study. The concentration of tocols was optimized at 22 MPa and 40C for palm oil. The selectivity of tocols follows the preference of δ ‐tocotrienol &gt; γ ‐tocotrienol &gt; α ‐tocotrienol &gt; α‐ tocopherol . PRACTICAL APPLICATIONS Crude palm oil (CPO) is unique among the common vegetable oils because of its high content of tocotrienols. Tocotrienols are more powerful antioxidants than tocopherols. Thus, CPO provides a good source for the production of tocols (tocopherol and tocotrienols) from a natural plant source. The solubility data obtained in the present study are important for the design and optimization of the supercritical fluid extraction of tocols because complex mixtures can lead to changes in solubility and mass transfer behavior. This study demonstrated that individual tocols have different maximum solubility and thus provide an alternative method for selective extraction of individual tocols.
Effect of Shock Wave Lithotripsy on Urease-Positive Calculogenic Bacteria (1988)

Extracorporeal shock wave lithotripsy has been extremely effective in patients with infection-associated renal calculi. Given recent data regarding the adverse effect of shock waves on malignant cells, the possibility was raised that shock waves might also have an adverse effect on calculogenic bacteria. Accordingly, we studied the direct and indirect effects of shock waves on urease-positive bacteria. In all instances, shock wave lithotripsy had no effect on viability of a variety of bacteria. These results more strongly underscore earlier recommendations that patients undergoing lithotripsy should have sterile urine. In those instances in which infection stones are suspected, appropriate parenteral antibiotic therapy should be instituted in order to sterilize the urine and should be continued in the immediate postlithotripsy period.
ChemInform Abstract: Ti/ITQ‐2, a New Material Highly Active and Selective for the Epoxidation of Olefins with Organic Hydroperoxides. (1999)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Multiple-antennas and isotropically-random unitary inputs: the received signal density in closed-form (2002)

Computing the capacity of a multiple antenna wireless link subject to flat Rayleigh block-fading, with no channel-state information available either to the transmitter or to the receiver, is an important open problem. The isotropically-random (i.r.) unitary matrix-having orthonormal columns, and a probability density that is invariant to pre-multiplication by an independent unitary matrix-plays a central role in the calculation of capacity and in some special cases is capacity-achieving. We take an important step towards computing this capacity by obtaining, in closed-form, the probability density of the received signal when transmitting i.r. unitary matrices. This enables us to evaluate the mutual information for any case of interest, something that could previously only be done for single transmit and receive antennas. Simulation results show that at high SNR the mutual information is maximized for M=min(N, T/2) transmit antennas, where N is the number of receive antennas and T is the length of the coherence interval, whereas at low SNR the mutual information is maximized by allocating all transmit power to a single antenna.
Management of type 2 diabetes with a treat-to-benefit approach improved long-term cardiovascular outcomes under routine care (2022)

Managing patients with T2D according to a "treat-to-benefit" approach based international standards was associated with a lower risk of MACE, heart failure, and mortality. These data provide ex-post validation of the ADA/EASD treatment algorithm.
Neural dysfunction and retinopathy in persons with type 1 diabetes (2018)

Sensory neuropathy and heartrate variability were significantly associated with prevalent PDR and ME in people with long-term T1D. PDR and ME were significantly associated with incident sensory neuropathy, and sensory neuropathy was significantly associated with incident PDR. Studies using earliest detectable markers of microvascular and neurologic abnormalities are needed to determine which of the two systems 'fails' first. Such information might suggest a temporal sequence of diabetes complications.
Collector Conductivity Modulation in 1200-V 4H-SiC BJTs (Modeling) (2017)

The resistance of the BJT collector layer can be sharply reduced by the effective injection of minority carriers (holes) from base to collector. As a result, the voltage drop across the BJT becomes substantially lower. The conditions under which this process can occur are the short rise time and the high amplitude of the base pulse.
Effects of Functionalized Gold Nanoparticle Size on X-ray Attenuation and Substrate Binding Affinity (2013)

Gold nanoparticles (Au NPs) have attracted interest as an X-ray contrast agent due to exhibiting high X-ray attenuation, colloidal stability, vascular retention, and facile surface functionalization for targeted delivery to cells and tissues. However, the effects of Au NP size on X-ray attenuation and binding affinity to a targeted surface are not well-understood. Therefore, the effect of Au NP size on X-ray attenuation was investigated by preparing mercaptosuccinic acid functionalized Au NPs exhibiting a mean particle diameter of 5, 13, 35, or 76 nm, as well as chloroauric acid control, at gold concentrations up to ∼50 mM (∼10 g/L). The X-ray attenuation of Au NP and chloroauric acid solutions increased with decreased photon energy and increased linearly with increased gold concentration, but was independent of the particle diameter. The effects of Au NP size on substrate binding affinity were investigated by preparing bisphosphonate functionalized Au NPs exhibiting a mean particle diameter of 5, 13, 35, or 76 nm and measuring binding isotherms using hydroxyapatite (HA) crystals as a model for bone mineral or microcalcifications. Decreased Au NP diameter resulted in an increased number of Au NPs but decreased mass of gold adsorbed onto HA crystal surfaces, and thus a lower binding affinity to HA. Therefore, the results of this study suggest that for targeted labeling of HA, or calcified tissue, an increased Au NP diameter will improve detection due to a greater of mass of gold labeling surfaces and thus greater X-ray attenuation.
Correction: Comprehensive Immunoprofiling of High-risk Oral Proliferative and Localized Leukoplakia (2022)

[This corrects the article DOI: 10.1158/2767-9764.CRC-21-0060.][This corrects the article DOI: 10.1158/2767-9764.CRC-21-0060.].
Novel Method for Covalent Fluorescent Labeling of Plasmid DNA That Maintains Structural Integrity of the Plasmid (1999)

We have developed a chemical strategy for covalent coupling of fluorophores to plasmid DNA. A p-azido-tetrafluoro-benzyl-lissamine conjugate was synthesized and purified. This conjugate was used to covalently associate fluorescent molecules to plasmid DNA by photoactivation. In contrast to nick-translated plasmid DNA, plasmid-lissamine conjugates appeared on gel as supercoiled DNA. Reporter gene was expressed after transfection of the plasmid-lissamine conjugates in NIH 3T3 cells, although gene transfer efficiency was decreased by 60% as compared with unlabeled DNA. Intracellular traffic of plasmid-lissamine conjugates was studied in transfected cells. After cytoplasmic microinjection, fluorescent plasmid did not diffuse from the site of injection and appeared to be progressively degraded in the cytoplasm.
Transcription profiling of early responses to hemorrhagic fever in rhesus macaque (2006)

Monkeys infected intravenously with a lethal dose of LCMV-WE provide a model for Lassa fever virus infection of man. Like Lassa fever in man, disease begins with flu-like symptoms and progresses rapidly to death within two weeks of infection. It is essential to correctly diagnose hemorrhagic fever rather than more benign viral diseases to provide the proper treatment. Microarray analyses were conducted on blood of macaques infected with virulent WE virus, and compared to gene expression in monkeys infected with non-virulent LCMV-ARM. We observed gene expression changes that occur before the viremic stage of the disease, and could potentially serve as biomarkers that discriminate between exposure to a hemorrhagic fever virus and exposure to a benign virus. In particular, transcription analysis revealed an early and severe suppression of the COX2 pathways indicating that disease treatments should avoid COX-inhibitors, like aspirin, and favor pathway stimulators.
User mobility impact on millimeter-wave system performance (2016)

In this paper we investigate the mobility effects impact on the millimeter-wave (mmWave) communication system performance. At the first step we introduce the mobility model on the example of the pedestrian motion and discuss its parameters for different environments. The impact of mobility on the system performance is estimated by comparison of channel properties vs. mmWave IEEE 802.11ad system parameters. The considered channel characteristics are calculated on the base of the Quasi-Deterministic (Q-D) channel model developed in MiWEBA project and available experimental measurement data. It is shown that mobile pedestrian channel is frequency-selective but stationary on the signal frame duration. The long-term mobility impact on the communication system performance is investigated on the PHY layer for signal bandwidths 500-2000 MHz. It is demonstrated that the system with wider bandwidth demonstrates 2-4 dB gain over the narrow-band one.
On Identifiability of Linear Time-Delay Systems (2002)

Identifiability analysis is developed for linear time-delay sys- tems with delayed states, control inputs, and measured outputs, all with a finite number of lumped delays. These systems are governed by linear func- tional differential equations with uncertain time-invariant parameters and delays. It is shown that the transfer function of such a system admits the online identification if a sufficiently nonsmooth input signal is applied to the system. Sufficiently nonsmooth signals are constructively defined by im- posing different smoothness properties on the control input and the state of the system. The required nonsmothness property is verified independently of any underlying time-delay system.
Calcium-Lipid Complexes in Human Platelets (1958)

Abstract Carefully washed human platelets contain calcium in an unusual state of chemical combination. Platelet calcium is non-ionic and is not exchangeable with ionic Ca45. On extraction of platelets with lipid solvents, the calcium separates with the phospholipids. The calcium-lipid complex thus obtained does not undergo any exchange with radioactive calcium. Study of other properties of platelets reveals that the clot-promoting activity of platelets (activated by antihemophilic factor and plasma thromboplastin component) also separates into the lipid fraction. Preliminary observations are reported on the nature of platelet lipids as revealed by paper chromatography and paper electrophoresis in solvents of low dielectric constant. Small amounts of proteolipid have been isolated from the lipid extracts. The unique calciumlipid complex is thought to originate in the cell membrane of the platelet.
Non-invasive screening for early Alzheimer’s disease diagnosis by a sensitively immunomagnetic biosensor (2016)

Amyloid-beta peptide 1-42 (Aβ42) is considered as a reliable biomarker for the early diagnosis of Alzheimer's disease (AD). Thus, it is urgent to develop a simple and efficient method for the detection of Aβ42. In this work, a reusable biosensor based on magnetic nitrogen-doped graphene (MNG) modified Au electrode for the detection of Aβ42 has been developed. The antibodies of Aβ 1-28 (Aβab) are used as the specific biorecognition element for Aβ42 that were conjugated on the surface of MNG. In the presence of magnetic nanoparticles on MNG, the electrode coating material, the biosensor can be quickly constructed, without requiring an electrode drying process, which reduce the analysis time and is convenient for proceeding to detection. The reusable biosensor with good reproducibility and stability was linear within the range from 5 pg mL(-1) to 800 pg mL(-1), covering the cut-off level of Aβ42 and a detection limit of 5 pg mL(-1) had been achieved. Furthermore, the fabricated biosensor for Aβ42 detection not only improves the detection performance but also reduces the cost and shortens the response time, demonstrating its potential in diagnosing applications.
Donor Desmopressin Is Associated With Superior Graft Survival After Kidney Transplantation (2011)

Donor DDAVP seems to improve renal allograft survival. Combined use of donor DDAVP and low-dose dopamine should receive further evaluation.
Automated campaign system (2006)

To run a targeted campaign involves coordination and management across numerous organizations and complex process flows. Everything from market analytics on customer databases, acquiring content and images, composing the materials, meeting the sponsoring enterprise brand standards, driving through production and fulfillment, and evaluating results; all processes are currently performed by experienced highly trained staff. Presented is a developed solution that not only brings together technologies that automate each process, but also automates the entire flow so that a novice user could easily run a successful campaign from their desktop. This paper presents the technologies, structure, and process flows used to bring this system together. Highlighted will be how the complexity of running a targeted campaign is hidden from the user through technologies, all while providing the benefits of a professionally managed campaign.