gebhart/patrepeval huggingface dataset:
The Universal IPv6 Configuration Option (2000)

One of the original intentions for the IPv6 host configuration, was to
configure the network-layer parameters only with IPv6 ND, and use
service discovery for other configuration information. Unfortunately
that hasn't panned out quite as planned, and we are in a situation
where all kinds of configuration options are added to RAs and DHCP.
This document proposes a new universal option for RA and DHCP in a
self-describing data format, with the list of elements maintained in
an IANA registry, with greatly relaxed rules for registration.
Video D from Preclinical Development of MGC018, a Duocarmycin-based Antibody–drug Conjugate Targeting B7-H3 for Solid Cancer (2023)

&lt;p&gt;5000 Hs700T B7-H3 KO/RFP cells alone in the presence of 6.7 nM MGC018&lt;/p&gt;
Phosphorylation coexists with O ‐GlcNAcylation in a plant virus protein and influences viral infection (2017)

Phosphorylation and O-GlcNAcylation are two widespread post-translational modifications (PTMs), often affecting the same eukaryotic target protein. Plum pox virus (PPV) is a member of the genus Potyvirus which infects a wide range of plant species. O-GlcNAcylation of the capsid protein (CP) of PPV has been studied extensively, and some evidence of CP phosphorylation has also been reported. Here, we use proteomics analyses to demonstrate that PPV CP is phosphorylated in vivo at the N-terminus and the beginning of the core region. In contrast with the 'yin-yang' mechanism that applies to some mammalian proteins, PPV CP phosphorylation affects residues different from those that are O-GlcNAcylated (serines Ser-25, Ser-81, Ser-101 and Ser-118). Our findings show that PPV CP can be concurrently phosphorylated and O-GlcNAcylated at nearby residues. However, an analysis using a differential proteomics strategy based on iTRAQ (isobaric tags for relative and absolute quantitation) showed a significant enhancement of phosphorylation at Ser-25 in virions recovered from O-GlcNAcylation-deficient plants, suggesting that crosstalk between O-GlcNAcylation and phosphorylation in PPV CP takes place. Although the preclusion of phosphorylation at the four identified phosphotarget sites only had a limited impact on viral infection, the mimicking of phosphorylation prevents PPV infection in Prunus persica and weakens infection in Nicotiana benthamiana and other herbaceous hosts, prompting the emergence of potentially compensatory second mutations. We postulate that the joint action of phosphorylation and O-GlcNAcylation in the N-proximal segment of CP allows a fine-tuning of protein stability, providing the amount of CP required in each step of viral infection.
Insulin-like growth factor-I increases expression of the porcine P-450 cholesterol side chain cleavage gene through a GC-rich domain. (1994)

The promoter/regulatory region of the porcine P-450 cholesterol side chain cleavage (P450scc) gene was cloned from a porcine genomic library. This gene contains a GC-rich region (-130-100) that mediates insulin-like growth factor I (IGF-I) and cAMP stimulation of gene expression in porcine granulosa cells. Stimulation of gene expression by cAMP occurs in 6 h, while IGF-I stimulation occurs in 24-48 h. This region is also responsive to insulin but not fibroblast growth factor. The effects of IGF-I and cAMP on gene expression in porcine granulosa cells are additive. In Y1 adrenal cells, the same region is responsive to cAMP but not to IGF-I. Gel shift assays using an oligonucleotide of this region and nuclear extract protein from porcine granulosa and Y1 adrenal cells identified three DNA-protein complexes (C1-C3). The binding activity of the complexes did not change with IGF-I or forskolin treatment of granulosa cells. Mutational analysis results were consistent with IGF-I regulating gene expression through the C2 DNA protein complex. Moreover, this complex binds differently in gel shift assay to mutant oligonucleotides with porcine and Y1 and nuclear extract protein. We conclude that IGF-I stimulates porcine P450scc gene expression through a distinct, cell-specific protein complex binding to a GC-rich domain.
ChemInform Abstract: Nickel‐Catalyzed Allylic Substitution of Simple Alkenes. (2011)

Abstract A variety of 1,4‐dienes is prepared with high regioselectivity by Ni—phosphine‐catalyzed allylic substitution of allylic alcohols, allylsilanes, vinylepoxides and vinyltetrahydrofuran derivatives with simple alkenes (in parts published previously).
Nucleation and Conformality of Iridium and Iridium Oxide Thin Films Grown by Atomic Layer Deposition (2016)

Nucleation and conformality are important issues, when depositing thin films for demanding applications. In this study, iridium and iridium dioxide (IrO<sub>2</sub>) films were deposited by atomic layer deposition (ALD), using five different processes. Different reactants, namely, O<sub>2</sub>, air, consecutive O<sub>2</sub> and H<sub>2</sub> (O<sub>2</sub> + H<sub>2</sub>), and consecutive O<sub>3</sub> and H<sub>2</sub> (O<sub>3</sub> + H<sub>2</sub>) pulses were used with iridium acetylacetonate [Ir(acac)<sub>3</sub>] to deposit Ir, while IrO<sub>2</sub> was deposited using Ir(acac)<sub>3</sub> and O<sub>3</sub>. Nucleation was studied using a combination of methods for film thickness and morphology evaluation. In conformality studies, microscopic lateral high-aspect-ratio (LHAR) test structures, specifically designed for accurate and versatile conformality testing of ALD films, were used. The order of nucleation, from the fastest to the slowest, was O<sub>2</sub> + H<sub>2</sub> > air ≈ O<sub>2</sub> > O<sub>3</sub> > O<sub>3</sub> + H<sub>2</sub>, whereas the order of conformality, from the best to the worst, was O<sub>3</sub> + H<sub>2</sub> > O<sub>2</sub> + H<sub>2</sub> > O<sub>2</sub> > O<sub>3</sub>. In the O<sub>3</sub> process, a change in film composition from IrO<sub>2</sub> to metallic Ir was seen inside the LHAR structures. Compared to the previous reports on ALD of platinum-group metals, most of the studied processes showed good to excellent results.
Thermotaxis of human trophoblastic cells (1996)

The objective of this study was to determine whether cultured human trophoblasts migrate in response to changes in oxygen tension or temperature. Human trophoblastic cells distributed homogenously within individual wells of standard culture plates were subjected to oxygen and thermal gradients. The redistribution of cells was determined 90 min to 18 h after these gradients had been established. Trophoblastic cells did not migrate in response to gradients of oxygen or carbon dioxide applied in this manner. In contrast, the cells migrated in response to thermal gradients of less than 1 degree C in the direction of the warmer temperature. The response began within minutes, was reversed by a change in the direction of the thermal gradient, and was inhibited at high cell concentrations. Migration was independent of proliferation or protein synthesis, but required microfilament assembly. The capacity of trophoblasts to migrate in response to small difference of temperature within the physiologic range may contribute to the initiation of placental development before contact with the maternal circulation has been established.
Chemical Synthesis of Astaxanthin n-Octanoic Acid Monoester and Diester and Evaluation of Their Oral Absorbability (2006)

We chemically synthesized astaxanthin n-octanoic acid monoester and diester from free astaxanthin and n-octanoic acid by a dehydration reagent in 32 and 22% yield, respectively. The oral absorbability of the n-octanoic acid monoester and diester was evaluated by examining the plasma and liver concentrations of astaxanthin after oral administration of the compounds. The monoester significantly increased the plasma and liver concentration of astaxanthin compared with the long-chain fatty acid ester mixture derived from Haematococcus algae. The diester is inclined to increase it although it is not significant. It is possible that medium-chain fatty acid esters give better oral-absorbability of astaxanthin than long-chain fatty acid esters.
Andrographolide downregulates pro‐inflammatory cytokines and free radical productions to prevent dopaminergic neuro‐degeneration induced by lipopolysaccharide (1143.16) (2014)

Oxidative stress and inflammation triggers the production of free radicals and toxic pro‐inflammatory cytokines leading to neurodegeneration. Down‐regulation of oxidative stress and inflammatory mediators offers defence against development and progression of the disease. Andrographolide is the most abundant diterpene lactone isolated from the leaves of Andrographis paniculata. Despite the increasing literature studies on the anti‐inflammatory effect of andrographolide, there is still paucity regarding its neuroprotective role as can be ascertained from the search of literature. The present study investigated the potential therapeutic role of andrographolide as a neuroprotective agent via in vitro model of lipopolysaccharide (LPS)‐induced brain injury using microglial (BV2) and dopaminergic (N27) cells. Pre‐treatment of andrographolide at non‐cytotoxic concentration range (0.25‐2.0 µM) of BV2 cells followed by LPS dose‐dependently suppressed nitric oxide production, pro‐inflammatory cytokines (TNF‐α, IL‐6) as well as attenuating intracellular reactive oxygen species (ROS) and thiobarbituric acid reactive substance (TBARS) compared to cells without andrographolide pretreatment. Further, conditioned media (CM) of the pretreatment had diminished toxicity towards dopaminergic neurons as compared to CM without pretreatment. Grant Funding Source : The Malaysian Ministry of Agriculture through NRGS grant allocation (NH0612D009)
Nearest-neighbor statistics for packings of hard spheres and disks (1995)

The probability of finding a nearest neighbor at some radial distance from a given particle in a system of interacting particles is of fundamental importance in a host of fields in the physical as well as biological sciences. A procedure is developed to obtain analytical expressions for nearest-neighbor probability functions for random isotropic packings of hard D-dimensional spheres that are accurate for all densities, i.e., up to the random close-packing fraction. Using these results, the mean nearest-neighbor distance \ensuremath{\lambda} as a function of the packing fraction is computed for such many-body systems and compared to rigorous bounds on \ensuremath{\lambda} derived here. Our theoretical results are found to be in excellent agreement with available computer-simulation data.
Figure S3 from Genetic Variants of <i>VEGFA</i> and <i>FLT4</i> Are Determinants of Survival in Renal Cell Carcinoma Patients Treated with Sorafenib (2023)

&lt;p&gt;Expression of WT and T494A VEGFR-3 in HUVECs (A) and replication of phosphorylation experiments testing the effect of FLT4 rs307826 (A&gt;G, T494A) on VEGFR-3 phosphorylation (B).&lt;/p&gt;
On chip ESD protection of 600V voltage node (2011)

This study presents for the first time ESD protection solutions in integrated silicon process technologies for the voltage range up to 600V. The ESD protection clamp is implemented using a NLDMOS-SCR type ESD device architecture. The study presents both reversible triggering I-V characteristics suitable for package level ESD protection as well as dependence of the ESD device characteristics upon the structure parameters and the state of a control electrode.
Effect of polyamines and basic proteins on cleavage of DNA by restriction endonucleases (1984)

We have investigated the effect of the polyamines spermine, spermidine, and putrescine and the prokaryotic histone-like proteins NS1 and NS2 on the restriction endonuclease EcoRI catalyzed cleavage of plasmid and bacteriophage DNAs. At low concentrations of spermine and spermidine, the rate of DNA cleavage by EcoRI is increased, while high concentrations of spermine as well as of spermidine are inhibitory. These phenomena are also observed with other restriction endonucleases. They are, therefore, probably due to the interaction of the polyamines with the DNA. Putrescine does not have such an effect within the concentration range investigated. Remarkably, low concentrations of spermine and spermidine very efficiently suppress EcoRI activity. An inhibition of the EcoRI-catalyzed cleavage of DNA is also observed with NS1 and NS2, an effect that can be mimicked with other basic proteins that interact with DNA. The results are discussed in terms of the mechanism of restriction in vivo.
Network transparency: the plaNET approach (1992)

Asynchronous transfer mode (ATM) is being suggested as the basis for future high speed, universal networks. A key requirement for future ATM networks will be transparency, i.e. for the network to alter or manipulate the user information as little as possible. A transparent transport mechanism, plaNET, based on extensions of the current ATM standard and IBM's earlier PARIS technology, is proposed. It is shown how plaNET can satisfy the universal transport requirement of ATM, while avoiding some demonstrated deficiencies. In fact, plaNET could be viewed as an enhanced version of ATM that may be more suitable for the late 1990s than the current standard.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Exploration of computer vision and image processing technology based on OpenCV (2022)

Face recognition is one of the important applications of computer vision. With the continuous development of computer technology and electronic information technology and the development of image recognition and pattern recognition, with the deepening of artificial intelligence research, face recognition has become a popular research topic. For the research of computer vision and image processing based on OpenCV, the essay uses a cascade classifier based on Haar-like feature for face detection and eye detection. Through the face recognition scheme based on principal component analysis (PCA) of AdaBoost, the eigenface and mean face are generated, and the face is reconstructed and recognized. In addition, the corresponding experiments and analysis of face detection and face recognition are also made, which achieves a high detection rate and recognition rate in the case of sufficient light.
Cross-Layer Resilience Against Soft Errors: Key Insights (2020)

Abstract Driven by technology scaling, integrated systems become more susceptible to various causes of random hardware faults such as radiation-induced soft errors. Such soft errors may cause malfunction of the system due to corruption of data or control flow, which may lead to unacceptable risks for life or property in safety-critical applications. Hence, safety-critical systems deploy protection techniques such as hardening and redundancy at different layers of the system stack (circuit, logic, architecture, OS/schedule, compiler, software, algorithm) to improve resiliency against soft errors. Here, cross-layer resilience techniques aim at finding lower cost solutions by providing accurate estimation of soft error resilience combined with a systematic exploration of protection techniques that work collaboratively across the system stack. This chapter demonstrates how to apply the cross-layer resilience principle on custom processors, fixed-hardware processors, accelerators, and SRAM memories (with a focus on soft errors) and presents key insights obtained.
Predicting changes in performance due to cognitive fatigue: A multimodal approach based on speech motor coordination and electrodermal activity (2020)

Results suggest that speech and arousal measures may be used to predict changes in performance associated with cognitive fatigue. This work supports ongoing efforts to develop reliable, unobtrusive measures for cognitive state assessment aimed at reducing injury risk, informing return to work decisions, and supporting diverse mobile healthcare applications in civilian and military settings.
Evidence for the multistep nature of in vitro human epithelial cell carcinogenesis. (1990)

In keeping with the multistep development of human cancer in vivo, a stepwise approach to neoplastic transformation in vitro presents a reasonable strategy. We have recently developed an in vitro multistep model suitable for the study of human epithelial cell carcinogenesis. Upon infection with the adenovirus 12-simian virus 40 hybrid virus, primary human epidermal keratinocytes acquired an indefinite life span in culture but did not undergo malignant conversion. Subsequent addition of Kirsten murine sarcoma virus and human ras oncogene or chemical carcinogens (N-methyl-N'-nitro-N-nitrosoguanidine or 4-nitroquinoline 1-oxide) to these cells induced morphological alterations and the acquisition of neoplastic properties. Subsequently it was found that this line could be transformed neoplastically by a variety of retrovirus-containing H-ras, bas, fes, fms, erbB, and src oncogenes. In addition, we found that the immortalized human epidermal keratinocyte (RHEK-1) line can be transformed neoplastically by exposure to ionizing radiation. Thus, this in vitro system may be useful in studying the interaction of a variety of carcinogenic agents and human epithelial cells. These findings demonstrate the malignant transformation of human primary epithelial cells in culture by the combined action of viruses, oncogenes, chemical carcinogens, or X-ray irradiation and support a multistep process for neoplastic conversion.
Modeling of Menisci and Capillary Forces from the Millimeter to the Micrometer Size Range (2000)

This paper examines lateral capillary interactions between millimeter- and sub-millimeter-sized objects floating at the interface between perfluorodecalin (PFD) and water. It describes methodsboth experimental and computationalthat allow the shape of the interface to be described for various geometries of the interacting objects. From these shapes, it derives the energy profiles characterizing the lateral capillary interactions. This work also demonstrates a new experimental method of imaging and studying menisci, and of studying capillary interactions between objects.
Decreasing Bacterial Colonization of External Fixation Pins Through Nitric Oxide Release Coatings (2020)

Bacterial infection of the pin tract represents the most common complication associated with external fixation. This study was designed to evaluate the antibacterial activity of nitric oxide (NO) releasing xerogel films applied to commercially pure titanium pins in a rat model.
Musical Melody Matching (2017)

I hope my critics will excuse me if I conclude from the opposite nature of their objections that I have struck out nearly the right path. As to my Theory of Consonance, I must claim it to be a mere systematisation of observed facts (with the exception of the functions of the cochlea of the ear, which is moreover an hypothesis that may be entirely dispensed with). But I consider it a mistake to make the Theory of Consonance the essential foundation of the Theory of Music, and, I had thought that this opinion was clearly enough expressed in my book. The essential basis of Music is Melody.—On the Sensations of Tone, Hermann Ludwig F. Helmholtz (1870)
Additional file 19: of Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus (2017)

Strain specific PFAM domains predicted for identified secreted proteins (A. sydowii, A. tubingensis, A. wentii, A. zonatus). (XLSX 12 kb)
Effects of Fuel Volatility and Operating Conditions on Fuel Sprays in DISI Engines: (1) Imaging Investigation (2000)

&lt;div class="htmlview paragraph"&gt;Optimal design of modern direct injection spark-ignition engines depends heavily on the characteristics and distribution of the fuel spray. This study was designed to investigate changes in the spray properties due to fuel volatility and operating conditions using a firing optically-accessible engine with planar laser-induced fluorescence (PLIF) imaging. The results show that the spray structure changes not only with ambient gas density, which is often measured, but also with fuel temperature and volatility. As ambient pressure decreases and fuel temperature increases, the volatile ends of multi-component fuels evaporate quickly, disrupting the spray structure and producing a vapor core along the axis of the spray. Beyond a certain point, evaporation is rapid enough to expand the initial cone angle of the spray while causing a decrease in the overall spray width. The axial and radial penetration of these sprays was found to depend on the fuel temperature, the ambient density, and vapor generation.&lt;/div&gt;
AMIGO2, a novel membrane anchor of PDK1, controls cell survival and angiogenesis via Akt activation (2015)

The phosphoinositide 3-kinase-Akt signaling pathway is essential to many biological processes, including cell proliferation, survival, metabolism, and angiogenesis, under pathophysiological conditions. Although 3-phosphoinositide-dependent kinase 1 (PDK1) is a primary activator of Akt at the plasma membrane, the optimal activation mechanism remains unclear. We report that adhesion molecule with IgG-like domain 2 (AMIGO2) is a novel scaffold protein that regulates PDK1 membrane localization and Akt activation. Loss of AMIGO2 in endothelial cells (ECs) led to apoptosis and inhibition of angiogenesis with Akt inactivation. Amino acid residues 465-474 in AMIGO2 directly bind to the PDK1 pleckstrin homology domain. A synthetic peptide containing the AMIGO2 465-474 residues abrogated the AMIGO2-PDK1 interaction and Akt activation. Moreover, it effectively suppressed pathological angiogenesis in murine tumor and oxygen-induced retinopathy models. These results demonstrate that AMIGO2 is an important regulator of the PDK1-Akt pathway in ECs and suggest that interference of the PDK1-AMIGO2 interaction might be a novel pharmaceutical target for designing an Akt pathway inhibitor.
Double-Sequence Frequency Synchronization for Wideband Millimeter-Wave Systems With Few-Bit ADCs (2019)

In this paper, we propose and evaluate a novel double-sequence low-resolution frequency synchronization method in millimeter-wave (mmWave) systems. In our system model, the base station uses analog beams to send the synchronization signal with infinite-resolution digital-to-analog converters. The user equipment employs a fully digital front end to detect the synchronization signal with low-resolution analog-to-digital converters (ADCs). The key ingredient of the proposed method is the custom designed synchronization sequence pairs, from which there exists an invertible function (a ratio metric) of the carrier frequency offset (CFO) to be estimated. We use numerical examples to show that the ratio metric is robust to the quantization distortion. To implement our proposed method in practice, we propose to optimize the double-sequence design parameters such that: (i) for each individual user, the impact of the quantization distortion on the CFO estimation accuracy is minimized, and (ii) the resulting frequency range of estimation can capture as many users' CFOs as possible. Numerical results reveal that our proposed algorithm can provide a flexible means to estimate CFO in a variety of low-resolution settings.
Development of Analytical Methods to Evaluate Sfh (1992)

For the determination of stroma content in SFH, two analytical methods were developed, the HPLC-UV for phospholipids and the enzyme immunoassay for blood group antigen. These analytical methods were applied to evaluate three representative SFH preparations. The 36,000 x g centrifugation method was shown to contain higher amount of stroma components. On the other hand, the BMM-40 nm and the ultrafiltration method with a 100 kd membrane was shown to remove more than 99.7% of phospholipids and almost all of blood group antigen from hemoglobin solutions. These results demonstrated that these analytical methods were very useful in evaluating any highly purified hemoglobin solutions.