gebhart/patrepeval huggingface dataset:
c-Myb Is an Evolutionary Conserved miR-150 Target and miR-150/c-Myb Interaction Is Important for Embryonic Development (2008)

Human c-Myb proto-oncogene is highly expressed in hematopoietic progenitors as well as leukemia and certain solid tumor. However, the regulatory mechanisms of its expression and biological functions remain largely unclear. Recently, c-Myb has been shown to be targeted by microRNA-150 (miR-150) which thereby controls B cell differentiation in mice. In this study, we demonstrated that c-Myb is an evolutionary conserved target of miR-150 in human and zebrafish, using reporter assays. Ectopic expression of miR-150 in breast cancer and leukemic cells repressed endogenous c-Myb at both messenger RNA (mRNA) and protein levels. Among several leukemia cell lines, primary leukemia cells, and normal lymphocytes, expression levels of miR-150 inversely correlated with c-Myb. The miR-150 overexpression or c-Myb silencing in zebrafish zygotes led to similar and serious phenotypic defects in zebrafish, and the phenotypic aberrations induced by miR-150 could be reversed by coinjection of c-Myb mRNA. Our findings suggest that c-Myb is an evolutionally conserved target of miR-150 and miR-150/c-Myb interaction is important for embryonic development and possibly oncogenesis.
Screening of Viral Pathogens from Pediatric Ileal Tissue Samples after Vaccination (2014)

In 2010, researchers reported that the two US-licensed rotavirus vaccines contained DNA or DNA fragments from porcine circovirus (PCV). Although PCV, a common virus among pigs, is not thought to cause illness in humans, these findings raised several safety concerns. In this study, we sought to determine whether viruses, including PCV, could be detected in ileal tissue samples of children vaccinated with one of the two rotavirus vaccines. A broad spectrum, novel DNA detection technology, the Lawrence Livermore Microbial Detection Array (LLMDA), was utilized, and confirmation of viral pathogens using the polymerase chain reaction (PCR) was conducted. The LLMDA technology was recently used to identify PCV from one rotavirus vaccine. Ileal tissue samples were analyzed from 21 subjects, aged 15-62 months. PCV was not detected in any ileal tissue samples by the LLMDA or PCR. LLMDA identified a human rotavirus A from one of the vaccinated subjects, which is likely due to a recent infection from a wild type rotavirus. LLMDA also identified human parechovirus, a common gastroenteritis viral infection, from two subjects. Additionally, LLMDA detected common gastrointestinal bacterial organisms from the Enterobacteriaceae, Bacteroidaceae, and Streptococcaceae families from several subjects. This study provides a survey of viral and bacterial pathogens from pediatric ileal samples, and may shed light on future studies to identify pathogen associations with pediatric vaccinations.
Topology synthesis of large‐displacement compliant mechanisms (2001)

Abstract This paper describes the use of topology optimization as a synthesis tool for the design of large‐displacement compliant mechanisms. An objective function for the synthesis of large‐displacement mechanisms is proposed together with a formulation for synthesis of path‐generating compliant mechanisms. The responses of the compliant mechanisms are modelled using a total Lagrangian finite element formulation, the sensitivity analysis is performed using the adjoint method and the optimization problem is solved using the method of moving asymptotes. Procedures to circumvent some numerical problems are discussed. Copyright © 2001 John Wiley &amp; Sons, Ltd.
Analysis of GNSS Signals as Observed via a High Gain Parabolic Antenna (2005)

There are a number of different error sources which corrupt the pseudorange measurements of navigation receivers. Multipath and noise remain difficult to erase and represent the most critical problem for several Global Navigation Satellite System (GNSS) receiver applications. However, for high performance applications such as GPS-based aircraft navigation and landing system, even smaller error contribution must be considered in the error budgets. For example, the minor differences between the signals broadcasted from the GPS satellites have to be detected and quantified. This paper describes an experiment performed in Green Bank, West Virginia, where a 110 meter high gain antenna was used to receive the signal broadcasted by GNSS satellites. The paper will present the most important results of the analysis toolset specifically developed to process the collected data. Diagrams and measurements will be shown, highlighting the differences between the signals from each of the satellites.
Metamaterial with polarization and direction insensitive resonant transmission response mimicking electromagnetically induced transparency (2009)

We report on a planar metamaterial, the resonant transmission frequency of which does not depend on the polarization and angle of incidence of electromagnetic waves. The resonance results from the excitation of high-Q antisymmetric trapped current mode and shows sharp phase dispersion characteristic to Fano-type resonances of the electromagnetically induced transparency phenomenon.
Observation of 13C–14N dipolar couplings in single crystals of glycine (1975)

Dipolar splittings are normally not observable because the large number of nuclear couplings usually present produces extensive broadening of the NMR lines. In this note the 13C–14N splitting in glycine is observed by using rf fields of sufficient strength to decouple the protons from the magnetically dilute nuclei. (AIP)
Fine pitch Cu pillar wafer process development and seed layer etching characterization (2012)

This paper will revise the traditional wafer fabrication process flow to accommodate the new material used to achieve 40 um fine pitch Cu pillar with minimize seed layer undercut. New photo-resist material is introduced to attain a single coating of 40um thickness and it has demonstrated the capability of attaining an aspect ratio of 2. The wafer fabrication process ended using a combination of seed layer (Ti/Cu) wet and dry etching. It has shown the potential of achieving almost zero undercut which is very critical for a 20um via. Cross-sectional SEM will be carried out to verify the side wall profile and the footing of the photo-resist. FIB cross-section is done to identify Ti and Cu undercut. Bump shear test will be performed after the seed layer etching to quantify the failure mode of a bump with and without seed layer undercuts.
Putting the Physical into the Digital: Issues in Designing Hybrid Interactive Surfaces (2009)

Hybrid surfaces are interactive systems combining techniques of direct-manipulation multi-touch surface interaction with elements of tangible user interfaces (TUIs). The design space for such complex hands-on computing experiences is sufficiently broad that it can be difficult to decide when interface elements should be given either a physical or digital instantiation, and the extent to which different interface functions should be made to model real-world interactions. In this paper we present two case studies of hybrid surface systems we are developing and discuss how we have reasoned through these kinds of design decisions. From this, we derive a set of observations about properties of physical and digital elements, and offer them as a design resource.
Miniaturization strategy of compact antenna using magneto-dielectric material (2019)

This article describes a strategy of miniaturization for an electrically small Monopolar Wire-Plate Antenna (MWPA) loaded by Magneto-Dielectric Material (MDM). The multiplication of antenna’s shorted wires lead to a stronger interaction with the material. An electrically small six wires MWPA loaded with MDM is designed at VHF band (sphere radius around λ 0 /15). While conserving interesting performances (η t =70%), the designed antenna is 27% smaller than a single shorted MWPA without loading material.
Activation of Acyl-Coenzyme A:Cholesterol Acyltransferase by Cholesterol or by Oxysterol in a Cell-free System (1995)

Acyl-coenzyme A:cholesterol acyltransferase (ACAT) is an intracellular enzyme that catalyzes the conjugation of long chain fatty acid and cholesterol to form cholesteryl esters. It is an integral membrane protein located in the endoplasmic reticulum. Experiments performed in intact mammalian cells have shown that the rate of cholesteryl ester synthesis in intact cells, as well as the ACAT activity from cell extracts, are greatly activated by the addition of low density lipoprotein (LDL) or oxygenated sterols such as 25-hydroxycholesterol to the growth medium. However, the molecular mechanism(s) by which sterol(s) stimulate the ACAT activity remains to be elucidated. Recently, our laboratory reported the expression cloning of human ACAT cDNA (Chang, C. C. Y., Huh, H. Y., Cadigan, K. M., and Chang, T. Y. 1993) J. Biol. Chem. 268, 20747-20755). In the current study, we report the expression of human ACAT cDNA in insect Sf9 cells. Uninfected Sf9 cells do not express detectable ACAT-like activity. Infecting these cells with recombinant virus containing ACAT cDNA caused these cells to express high levels of ACAT protein and high levels of ACAT activity when assayed in vitro. The catalytic properties of ACAT expressed in these cells were found to be similar to those found in human tissue culture cells. The combination of high level of ACAT protein expression and the low level of cellular cholesterol content in the infected cells have provided us a novel opportunity to establish a simple cell-free system, whereby stimulation of ACAT by sterols can be readily demonstrated. Using this system, we have shown that cholesterol itself can serve as an ACAT activator in vitro, in addition to its role as an ACAT substrate. The current work provides the experimental basis to hypothesize that, inside mammalian cells, cholesterol itself may serve as a physiological regulator of ACAT.
A polyamine coating for enhanced capillary electrophoresis‐electrospray ionization‐mass spectrometry of proteins and peptides (2004)

A procedure for enhanced capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) of proteins is presented. The use of a newly presented capillary coating, PolyE-323, provided fast separations of typically a few minutes with high efficiency, good deactivation, and no bleeding into the mass spectrometer. Capillaries coated with PolyE-323 showed high stability over a range of pH 2-10, and tolerance towards methanol and acetonitrile, two modifiers commonly used in CE-ESI-MS. Due to the speed and simplicity of the coating procedure, the polymeric surface could, if necessary, easily be regenerated. This capability is especially valuable when working with samples of complex matrix, where a capillary surface cleaning step might be desired in order to eliminate possible memory effects. The potential of PolyE-323-coated capillaries in bioanalysis using CE-ESI-MS was demonstrated by analyzing peptides and proteins up to 66 kDa using time of flight (TOF)-MS. Due to the stable, anodal electroosmotic flow generated by the coating, the use of a sheathless ESI interface was enabled, demonstrated in peptide analysis with attomole sensitivity. The fast on-line CE-ESI-TOF system using PolyE-323-coated capillaries provided efficient separation and detection of a large number of peaks in a short time, exemplified by the analysis of a tryptic digest of bovine serum albumin (BSA). The capability of the developed capillary surface coating was demonstrated by the separation of human plasma and cerebrospinal fluid (CSF).
Reduced Peripheral Nerve Function Is Related to Lower Hip BMD and Calcaneal QUS in Older White and Black Adults: The Health, Aging, and Body Composition Study (2006)

Poor peripheral nerve function may directly related to lower BMD, likely through an increase in bone area in older adults, independent of lean mass, strength, physical ability, and diabetes. Whether those with impaired nerve function are at higher risk for fracture independent of falls needs to be studied.
A high-responsivity high-bandwidth asymmetric twin-waveguide coupled InGaAs-InP-InAlAs avalanche photodiode (2002)

The authors demonstrate an integratable high-responsivity high-bandwidth long wavelength InGaAs-InP-InAlAs avalanche photodiode based on an asymmetric twin-waveguide structure. The device has an external quantum efficiency of 48 /spl plusmn/ 1.5% at /spl lambda/ = 1.55 μm, a 3-dB bandwidth of 28.5 /spl plusmn/ 0.5 GHz with gain of up to 4, and a fiber-to-waveguide misalignment tolerance of /spl plusmn/ 1.0 μm in the vertical and /spl plusmn/ 1.3 μm in the horizontal directions resulting in a 1-dB sensitivity penalty.
Influence of mTOR Signalling Pathway on the MHC I Immunopeptidome. (2007)

Abstract Cell surface major histocompatibility complex (MHC) I molecules are associated with self peptides that are collectively referred to as the self MHC I immunopeptidome (sMII). Despite the tremendous importance of the sMII, very little is known on its genesis and molecular composition. On the other hand, it is well established that the signalling pathway involving mammalian target of rapamycin (mTOR) plays an essential role in the regulation of processes such as ribosome biogenesis and protein translation which are critical for cell growth, proliferation and differentiation. In this work, we studied the influence of mTOR on the sMII for two major reasons: the tremendous importance of this pathway in oncogenic processes its role in the control of protein synthesis, which is at the origin of the generation of the sMII. To achieve this goal, we developed a novel high-throughput mass spectrometry approach that yields an accurate definition of the nature and relative abundance of unlabeled peptides presented by MHC I molecules. Starting from EL4 thymoma peptide extracts, more than 200 MHC I-associated peptides were sequenced with high confidence level across more than 5500 peptide clusters reproductibly identified through replicate injections (n = 3). Comparison of the sMII of EL4 thymomas before and after rapamycin stimulation revealed that 13% of the MHC I-associated peptides were significant overexpressed (fold change ≥ 2, p-value ≤ 0.05) after mTOR inhibition. Out of the 27 MHC I peptide candidates showing differential expression, 60% of peptide source proteins were linked to cell development and/or proliferation including ITFIFKSL and NAIKNHWNSTM assigned to FK506 binding protein 12 (mTOR) and myeloblastosis oncogene (c-myb) proteins, respectively. These results indicate that cell signalling events have a major impact on the composition of the sMII that can be monitored by analyses of high-throughput MS-based quantitative profiles. Figure 1: Relative Quantification of Differentially Expressed MHC I peptides. Volcano Plot representation illustrates MHC I peptides reproductibly detected across replicate injections (n=3). Peptides over-expressed on EL4 cells after rapamycin stimulation are highlighted in blue. Figure 1:. Relative Quantification of Differentially Expressed MHC I peptides. Volcano Plot representation illustrates MHC I peptides reproductibly detected across replicate injections (n=3). Peptides over-expressed on EL4 cells after rapamycin stimulation are highlighted in blue.
Electrochemical imaging of fusion pore openings by electrochemical detector arrays (2005)

Opening of individual exocytotic fusion pores in chromaffin cells was imaged electrochemically with high time resolution. Electrochemical detector arrays that consist of four platinum microelectrodes were microfabricated on a glass coverslip. Exocytosis of single vesicles containing catecholamines from a cell positioned on top of the array is detected by the individual electrodes as a time-resolved oxidation current, reflecting the time course of arrival of catecholamine molecules at the electrode surfaces. The signals exhibit low noise and reveal foot signals indicating fusion pore formation and expansion. The position of individual release events is determined from the fraction of catecholamines recorded by the individual electrodes. Simultaneous fluorescence imaging of release of acridine orange from individual vesicles confirmed the electrochemical position assignments. This electrochemical camera provides very high time resolution, spatiotemporal localization of individual fusion pore openings and quantitative data on the flux of transmitter from individual vesicles. Analysis of the amperometric currents employing random walk simulations indicates that the time course of amperometric spikes measured near the cell surface is due to a low apparent diffusion coefficient of catecholamines near the cell surface and not due to slow dissociation from the granular matrix.
Unitarity-conserving construction of the Jones matrix and its application to polarization-mode dispersion analysis (1997)

We propose a method to measure the Jones matrix of the transmission medium without violating the unitarity of the matrix. Our method consists of defining the normalized Jones matrix each time the unitarity is lost by experimental error. Numerical simulation is performed to show the feasibility of the proposed method. The method is applied to polarization-mode dispersion analysis. We derive an explicit expression that allows us to evaluate the polarization-mode dispersion vector directly from the elements of the Jones matrix. We also introduce a parameter ∑ to determine a suitable wavelength step size for the measurement of the differential group delay of the principal states of polarization.
Extracorporeal membrane oxygenation without therapeutic anticoagulation in adults: A systematic review of the current literature (2020)

Extracorporeal membrane oxygenation is essential for the treatment of refractory cardiopulmonary failure. Its use may be complicated by worse haemorrhagic complications exacerbated by extracorporeal membrane oxygenation-related therapeutic anticoagulation. Progressive technological advancements have made extracorporeal membrane oxygenation components less thrombogenic, potentially allowing its application with temporary avoidance of systemic anticoagulants. A systematic review of all the available experiences, reporting the use of extracorporeal membrane oxygenation without systemic anticoagulation in the published literature was performed. Only patient series were included, irrespective of the clinical indication. The survival, extracorporeal membrane oxygenation system-related dysfunction and complications rates, as well as in-hospital outcome, were analysed. Six studies were selected for the analysis. Veno-arterial extracorporeal membrane oxygenation was used in 84% of patients, while veno-venous extracorporeal membrane oxygenation was applied in the remaining cases. Anticoagulation was avoided because of the high risk of bleeding after cardiac surgery (64%), active major bleeding (23%) or presence of severe traumatic injury (9%). Duration of support ranged from 0.3 to 1128 h. Heparin was antagonized by protamine in all the post-cardiotomy cases. Successfully extracorporeal membrane oxygenation weaning was achieved in 74% of the treated cases, with a hospital discharge of 58% of patients. Rates of extracorporeal membrane oxygenation malfunctioning due to clot formation and blood transfusion requirement varied remarkably in the published series. Extracorporeal membrane oxygenation without systemic anticoagulation appears feasible in selected circumstances. Further investigations are warranted to elucidate actual aspects regarding extracorporeal membrane oxygenation system performance, related adverse events and benefits associated with this management.
Multiple Bonds between Transition Metals and Main-Group Elements. 145. Coordination Chemistry of Dirhenium Heptaoxide: Covalent Adducts and "Ionic Perrhenyl-Perrhenates" (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMultiple Bonds between Transition Metals and Main-Group Elements. 145. Coordination Chemistry of Dirhenium Heptaoxide: Covalent Adducts and "Ionic Perrhenyl-Perrhenates"Wolfgang A. Herrmann, Peter W. Roesky, Fritz E. Kuehn, Martina Elison, Georg Artus, Wolfgang Scherer, Carlos C. Romao, Andre Lopes, and Jean-Marie BassetCite this: Inorg. Chem. 1995, 34, 19, 4701–4707Publication Date (Print):September 1, 1995Publication History Published online1 May 2002Published inissue 1 September 1995https://doi.org/10.1021/ic00123a001RIGHTS & PERMISSIONSArticle Views289Altmetric-Citations29LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (805 KB) Get e-AlertsSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
On building a concatenative speech synthesis system from the blizzard challenge speech databases (2005)

In this paper, we compare two methods of building a concatenative speech synthesis system from the relatively small, "Blizzard Challenge" speech databases.In the first method we build a system directly from the Blizzard databases using the IBM Concatenetative Speech Synthesis System originally designed for very large speech databases.In the second method, a larger database is used to build the synthesis system and the output is "morphed" to match the speakers in the Blizzard databases.The second method outperformed the first while maintaining the identity of the Blizzard target speakers.
Hypoxia in the Androgen-Dependent Shionogi Model for Prostate Cancer at Three Stages1 (2004)

The objective of this study was to investigate a possible relationship between androgen status and hypoxia in the Shionogi murine prostate tumor model, which is widely used to study the effects of androgen withdrawal on hormone resistance and radiation response. Binding of the nitroimidazole hypoxia marker EF5 was assessed using the Cy3-tagged monoclonal antibody ELK3-51. Three hours after injection of EF5 (30 mg/kg), tumors from the following three stages were excised: androgen-dependent, regressed tumors 7 days after castration, and androgen-independent. Half of each tumor was disaggregated for analysis by flow cytometry and the remainder was flash frozen. Statistically significant differences (P < 0.01) were found between androgen-dependent, regressed and androgen-dependent tumors: approximately 30, approximately 2 and approximately 50% hypoxic cells, respectively. Frozen sections from androgen-dependent tumors exhibited highly variable EF5 binding; regressed tumors showed very little or no binding; each section from androgen-dependent tumors showed high levels and uniformly distributed binding of EF5. There was no correlation between the degree of hypoxia and tumor weight (P > 0.1). The results from this preliminary study indicate that hypoxia may play an important role with respect to the timing of irradiation in prostate cancer treatments and possibly may be a useful prognostic tool. In addition, hypoxia may also be relevant to progression in this disease after androgen ablation.