gebhart/patrepeval huggingface dataset:
Glaucoma-causing myocilin mutants require the Peroxisomal targeting signal-1 receptor (PTS1R) to elevate intraocular pressure (2007)

Glaucoma is a leading cause of worldwide irreversible visual impairment and blindness and is a clinically and genetically heterogenous group of optic neuropathies. Specific mutations in the myocilin (MYOC) gene cause primary open angle glaucoma (POAG) with varying age-of-onset and degree of severity. We show a mutation-dependent, gain-of-function association between human myocilin and the peroxisomal targeting signal type 1 receptor (PTS1R). There was correlation between the glaucoma phenotype and the specific MYOC mutations, with the more severe early-onset POAG mutations having a higher degree of association with PTS1R. Expression of human myocilin glaucomatous mutations in mouse eyes causes elevated intraocular pressure, which is a major phenotype of MYOC glaucoma. This is the first demonstration of a disease resulting from mutation-induced exposure of a cryptic signaling site that causes mislocalization of mutant protein to peroxisomes and the first disease-gene-based animal model of human POAG.
Functionalized Fluorinated Allenes (2005)

An overview of the literature of mono- and di-fluorinated allenes is presented with an emphasis on the synthesis and chemistry of silyl substituted mono- and di-fluorinated allenes and fluoroallenylphosphonates.
Deployable Euler Spiral Connectors (2021)

Abstract Deployable Euler spiral connectors (DESCs) are introduced as compliant deployable flexures that can span gaps between segments in a mechanism and then lay flat when under strain in a stowed position. This paper presents models of Euler spiral beams combined in series and parallel that can be used to design compact compliant mechanisms. Constraints on the flexure parameters of DESCs are also presented. Analytic models developed for the force-deflection behavior and stress were compared to finite element analysis and experimental data. A spinal implant and a linear ratcheting system are presented as illustrative applications of DESCs.
Coupled thermo-optical modeling of high power operation of Tm, Ho:YLF solid-state lasers (2007)

Integrated computational model for operation of co-doped Tm,Ho solid-state lasers is developed coupling (i) 8-level rate equations with (ii) TEM00 laser beam distribution, and (iii) complex heat dissipation model. Simulations done for Q-switched &ap;0.1 J giant pulse generation by Tm,Ho:YLF laser show that &ap;43 % of the 780 nm light diode side-pumped energy is directly transformed into the heat inside the crystal, whereas &ap;45 % is the spontaneously emitted radiation from <sup>3</sup>F<sub>4</sub>, <sup>5</sup>I<sub>7</sub> , <sup>3</sup>H<sub>4</sub> and <sup>3</sup>H<sub>5</sub> levels. In water-cooled operation this radiation is absorbed inside the thermal boundary layer where the heat transfer is dominated by heat conduction. In high-power operation the resulting temperature increase is shown to lead to (i) significant decrease in giant pulse energy and (ii) thermal lensing.
Faculty Opinions recommendation of Conversion of PtdIns(4,5)P(2) into PtdIns(5)P by the S.flexneri effector IpgD reorganizes host cell morphology. (2002)

Phosphoinositides play a central role in the control of several cellular events including actin cytoskeleton organization.Here we show that, upon infection of epithelial cells with the Gram-negative pathogen Shigella exneri, the virulence factor IpgD is translocated directly into eukaryotic cells and acts as a potent inositol 4-phosphatase that specically dephosphorylates phosphatidylinositol 4,5-bisphosphate [PtdIns-(4,5)P 2 ] into phosphatidylinositol 5-monophosphate [PtdIns(5)P] that then accumulates.Transfection experiments indicate that the transformation of PtdIns(4,5)P 2 into PtdIns(5)P by IpgD is responsible for dramatic morphological changes of the host cell, leading to a decrease in membrane tether force associated with membrane blebbing and actin lament remodelling.These data provide the molecular basis for a new mechanism employed by a pathogenic bacterium to promote membrane rufing at the entry site.
Field-capable biodetection devices for homeland security missions (2007)

Biodetection instrumentation that is capable of functioning effectively outside the controlled laboratory environment is critical for the detection of health threats, and is a crucial technology for Health Security. Experience in bringing technologies from the basic research laboratory to integrated fieldable instruments suggests lessons for the engineering of these systems. This overview will cover several classes of such devices, with examples from systems developed for homeland security missions by Lawrence Livermore National Laboratory (LLNL). Recent trends suggest that front-end sample processing is becoming a critical performance-determining factor for many classes of fieldable biodetection devices. This paper introduces some results of a recent study that was undertaken to assess the requirements and potential technologies for next-generation integrated sample processing.
Potent Quinoline-Containing Combretastatin A-4 Analogues: Design, Synthesis, Antiproliferative, and Anti-Tubulin Activity (2020)

A novel series of quinoline derivatives of combretastatin A-4 incorporating rigid hydrazone and a cyclic oxadiazole linkers were synthesized and have demonstrated potent tubulin polymerization inhibitory properties. Many of these novel derivatives have shown significant antiproliferative activities in the submicromolar range. The most potent compound, <b>19h</b>, demonstrated superior IC<sub>50</sub> values ranging from 0.02 to 0.04 µM against four cancer cell lines while maintaining low cytotoxicity in MCF-10A non-cancer cells, thereby suggesting <b>19h</b>'s selectivity towards proliferating cancer cells. In addition to tubulin polymerization inhibition, <b>19h</b> caused cell cycle arrest in MCF-7 cells at the G2/M phase and induced apoptosis. Collectively, these findings indicate that <b>19h</b> holds potential for further investigation as a potent chemotherapeutic agent targeting tubulin.
ChemInform Abstract: Molecular Design of Novel PGI2 Agonists Without PG Skeleton. Part 2. (1995)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Performance Analysis of Cooperative Spectrum Sensing in Suzuki Fading Channels (2007)

This paper examines the performance of cooperative spectrum sensing, using energy detection, in Suzuki fading channels. Sub-optimal centralized detection approaches are examined where decisions are made based on identical tests performed at the individual radios. Decisions are performed at a fusion center using a counting rule that encompasses the OR, AND, and majority rules as special cases. Analytical and simulation results are presented for Rayleigh, Log-normal and Suzuki distributions.
Identification of African Horse Sickness Virus in Cell Culture using a Digoxigenin-Labeled RNA Probe (1994)

A digoxigenin-labeled RNA probe was synthesized from a plasmid containing a portion of the African horse sickness virus (AHSV) serotype 4 genome segment coding for nonstructural protein 1. In an in situ hybridization procedure, this probe hybridized successfully to Vero cells infected with each of the 9 serotypes of AHSV. There was no hybridization with noninfected cell cultures or cell cultures infected with bluetongue virus.
CORNEAL STRUCTURE AND FUNCTION (2011)

Jeffrey W. Ruberti, Ph.D.; Associate Professor, Mechanical and Industrial Engineering, Northeastern University, Boston, MA; email: j.ruberti@neu.edu Abhijit Sinha Roy, Ph.D.; Department of Ophthalmology, Cleveland Clinic Cole Eye Institute, Cleveland, OH 44195; email: asroy27@yahoo.com Cynthia J. Roberts, Ph.D.; Departments of Ophthalmology and Biomedical Engineering, The Ohio State University, Columbus, Ohio 43210; email: roberts.8@osu.edu
Reverse engineering a predictive signature characterized by proliferation, DNA damage, and immune escape from stage I lung adenocarcinoma recurrence (2020)

Identifying early-stage cancer patients at risk for progression is a major goal of biomarker research. This report describes a novel 19-gene signature (19-GCS) that predicts stage I lung adenocarcinoma (LAC) recurrence and response to therapy and performs comparably in pancreatic adenocarcinoma (PAC), which shares LAC molecular traits. Kaplan-Meier, Cox regression, and cross-validation analyses were used to build the signature from training, test, and validation sets comprising 831 stage I LAC transcriptomes from multiple independent data sets. A statistical analysis was performed using the R language. Pathway and gene set enrichment were used to identify underlying mechanisms. 19-GCS strongly predicts overall survival and recurrence-free survival in stage I LAC (P=0.002 and P<0.001, respectively) and in stage I-II PAC (P<0.0001 and P<0.0005, respectively). A multivariate cox regression analysis demonstrated the independence of 19-GCS from significant clinical factors. Pathway analyses revealed that 19-GCS high-risk LAC and PAC tumors are characterized by increased proliferation, enhanced stemness, DNA repair deficiency, and compromised MHC class I and II antigen presentation along with decreased immune infiltration. Importantly, high-risk LAC patients do not appear to benefit from adjuvant cisplatin while PAC patients derive additional benefit from FOLFIRINOX compared with gemcitabine-based regimens. When validated prospectively, this proof-of-concept biomarker may contribute to tailoring treatment, recurrence reduction, and survival improvements in early-stage lung and pancreatic cancers.
Engineering Saccharomyces cerevisiae for high-level synthesis of fatty acids and derived products (2017)

Fatty acids and fatty acid derivatives are important biorenewable products, as well as precursors for further transformation via chemical catalysis. This minireview focuses on recent advances in increasing the production of fatty acids and derived products in the yeast Saccharomyces cerevisiae. The engineering of upstream pathways to increase levels of the required precursors, fatty acid synthase systems to increase expression and to modify chain length, and downstream pathways to produce free fatty acids, fatty acid ethyl esters, fatty alcohols and alkanes are highlighted, and current challenges are discussed.
Investigation of Carrier Transport Mechanism in High Mobility ZnON Thin-Film Transistors (2016)

In this letter, the carrier transport mechanism in a high-mobility zinc oxynitride (ZnON) thin-film transistor (TFT) is investigated by analyzing the gate bias and temperature dependence of conductance and intrinsic field-effect mobility ( <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\mu _{\mathrm {FEi}})$ </tex-math></inline-formula> in the subthreshold and above-threshold regions, respectively. The measured drain currents increase with a temperature and show a thermally activated Arrhenius-like behavior in the subthreshold region. The experimental results are well explained using a Meyer–Neldel rule, which suggests that the trap-limited conduction is the dominant carrier transport mechanism in the ZnON TFT in the subthreshold region. The carrier transport mechanism in the ZnON TFT in the above-threshold region is investigated by examining the gate overdrive voltage ( <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$V_{\mathrm {OV}})$ </tex-math></inline-formula> and temperature dependence of <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\mu _{\mathrm {FEi}}$ </tex-math></inline-formula> . <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\mu _{\mathrm {FEi}}$ </tex-math></inline-formula> extracted from the ZnON TFT decreases with an increase in <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$V_{\mathrm {OV}}$ </tex-math></inline-formula> and temperature, which suggests that the phonon scattering is the most probable mechanism limiting <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\mu _{\mathrm {FEi}}$ </tex-math></inline-formula> in the ZnON TFT in the above-threshold region.
Constant Phase Element Analysis of Ordered Carbon Electrodes Via Electrochemical Impedance Spectroscopy and Cyclic Voltammetry (2021)

Electrochemical double layer capacitors exhibit constant phase element (CPE) behavior in both their cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) response. Recently, Allagui et. al [ Sci. Rep. , vol. 7, September, p. 46828, 2017.] presented equations for finding CPE parameters from CV measurements. EIS data for porous electrodes exhibiting CPE behavior can be fit using the de Levie model. In this work, several ordered carbon electrodes of varying pore length were prepared by chemical vapor deposition (CVD) of carbon nanotubes (CNTs) within anodized aluminum oxide (AAO) templates. These electrodes were used to create supercapacitors with neat EMIM-BF4 ionic liquid electrolyte. CPE parameters Q and α were found for each device from both CV and EIS, and they are in good agreement between the two methods. However, the values of resistance, another fitting parameter in both models, vary drastically. The physical origin of these resistances is discussed. Additionally, the capacitance of the devices was calculated from both methods, using the Brug formula in the case of EIS. These capacitances disagree greatly, except for the thickest AAO templates (the longest pores). The devices in agreement also showed the lowest resistance values from CV. It is not apparent whether the agreement in capacitance is related to the pore dimensions or the extracted resistance values. {DISTRIBUTION STATEMENT A. Approved for public release; Distribution is unlimited 412TW-PA-20496]
Pulmonary functional MRI: Detecting the structure–function pathologies that drive asthma symptoms and quality of life (2022)

Pulmonary functional MRI (PfMRI) using inhaled hyperpolarized, radiation-free gases (such as <sup>3</sup> He and <sup>129</sup> Xe) provides a way to directly visualize inhaled gas distribution and ventilation defects (or ventilation heterogeneity) in real time with high spatial (~mm<sup>3</sup> ) resolution. Both gases enable quantitative measurement of terminal airway morphology, while <sup>129</sup> Xe uniquely enables imaging the transfer of inhaled gas across the alveolar-capillary tissue barrier to the red blood cells. In patients with asthma, PfMRI abnormalities have been shown to reflect airway smooth muscle dysfunction, airway inflammation and remodelling, luminal occlusions and airway pruning. The method is rapid (8-15 s), cost-effective (~$300/scan) and very well tolerated in patients, even in those who are very young or very ill, because unlike computed tomography (CT), positron emission tomography and single-photon emission CT, there is no ionizing radiation and the examination takes only a few seconds. However, PfMRI is not without limitations, which include the requirement of complex image analysis, specialized equipment and additional training and quality control. We provide an overview of the three main applications of hyperpolarized noble gas MRI in asthma research including: (1) inhaled gas distribution or ventilation imaging, (2) alveolar microstructure and finally (3) gas transfer into the alveolar-capillary tissue space and from the tissue barrier into red blood cells in the pulmonary microvasculature. We highlight the evidence that supports a deeper understanding of the mechanisms of asthma worsening over time and the pathologies responsible for symptoms and disease control. We conclude with a summary of approaches that have the potential for integration into clinical workflows and that may be used to guide personalized treatment planning.
Generation of a Monoclonal Antibody to Detect Elastin-like Polypeptides (2019)

The identification and use of antibodies dominate the biologic, clinical diagnostic, and therapeutic landscapes. In particular, antibodies have become essential tools in a variety of protein analytical experiments and to study the disposition of biologic therapeutics. One emerging class of peptide biologics is known as the elastin-like polypeptides (ELPs), which are repetitive protein polymers inspired by human tropoelastin. A major limitation in the clinical translation of ELP biologics has been a lack of a monoclonal antibody (mAb) to characterize their identity during expression. To facilitate these studies, we successfully generated a new mAb that is specific toward ELPs and ELP fusion proteins. A purified antibody was evaluated in an ELISA, western blotting, and immunofluorescence assay. The optimal anti-ELP mAb proved to be highly reactive and specific toward ELPs. Moreover, they were able to detect ELPs with a variety of aliphatic guest residues. ELPs phase-separate in response to heating; furthermore, when incubated at a great excess of ELPs, the anti-ELP mAb partially blocks phase separation. These findings are direct evidence that novel murine mAbs can be raised against purified ELPs. This new reagent will enable purification, experimental detection, and characterization of these biopolymers.
P‐137: Developing Fast Bistable FLC Mixtures (2020)

Ferroelectric liquid crystals (FLCs), bistable when used in obliquely‐deposited SiO 2 cells, are being developed with the goal of obtaining fast‐switching microdisplays that have about 90% illumination duty cycle. The effects of changing various SiO 2 deposition parameters were investigated. A functional bistable FLC dummy cell is demonstrated.
Promoter analysis of an African Swine Fever Virus gene encoding a putative elongation factor (1995)

Conference Article| February 01 1995 Promoter analysis of an African Swine Fever Virus gene encoding a putative elongation factor PAULA R. YATES; PAULA R. YATES 1Department of Biochemistry, University of Liverpool, P.O. Box 147, Liverpool, L69 3BX, U.K. Search for other works by this author on: This Site PubMed Google Scholar LINDA K. DIXON; LINDA K. DIXON *BBSRC Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Woking, Surrey, GU24 0NF, U.K. Search for other works by this author on: This Site PubMed Google Scholar PHILIP. C. TURNER PHILIP. C. TURNER 1Department of Biochemistry, University of Liverpool, P.O. Box 147, Liverpool, L69 3BX, U.K. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1995) 23 (1): 139S. https://doi.org/10.1042/bst023139s Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation PAULA R. YATES, LINDA K. DIXON, PHILIP. C. TURNER; Promoter analysis of an African Swine Fever Virus gene encoding a putative elongation factor. Biochem Soc Trans 1 February 1995; 23 (1): 139S. doi: https://doi.org/10.1042/bst023139s Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1995 Biochemical Society1995 Article PDF first page preview Close Modal You do not currently have access to this content.
Using a novel four-port interleaver to enable unidirectional amplification in a 210-km bidirectional transmission system (2005)

We proposed and experimentally demonstrated a bidirectional transmission system by using a novel four-port interleaver to enable unidirectional amplification. After 210-km transmission, less than 0.2-dB sensitivity variations were observed between bidirectional and unidirectional transmissions
Biradical Polarizing Agents at High Fields (2022)

The sensitivity enhancements available from dynamic nuclear polarization (DNP) are rapidly reshaping the research landscape and expanding the field of nuclear magnetic resonance (NMR) spectroscopy as a tool for solving complex chemical and structural problems. The past decade has seen considerable advances in this burgeoning method, while efforts to further improve its capabilities continue along many avenues. In this report, we examine the influence of static magnetic field strength and temperature on the reported <sup>1</sup>H DNP enhancements from three conventional organic biradicals: TOTAPOL, AMUPol, and SPIROPOL. In contrast to the conventional wisdom, our findings show that at liquid nitrogen temperatures and 700 MHz/460.5 GHz, these three bisnitroxides all provide similar <sup>1</sup>H DNP enhancements, ε ≈ 60. Furthermore, we investigate the influence of temperature, microwave power, magnetic field strength, and protein sample deuteration on the NMR experimental results.
A Novel Carbonyldiniobium Complex with Hydrogen Bridge (1981)

The first homodinuclear carbonyl compound , the hydrido complex (3) , was obtained in a light‐induced reaction from the newly accessible (1) and (2) . The (η 5 ‐C 5 H 5 ) 2 Nb(CO) 3 ‐ and (η 5 ‐C 5 H 5 )Nb(CO)‐fragments are coupled by a slightly bent H‐bridge. equation image
Macroblock sampling and mode ranking for complexity scalability in mobile H.264 video coding (2009)

We propose a framework for complexity scalability in H.264. The prediction is constrained so that only a subset of prediction modes are tested. The test subset is found by ranking the most ¿popular¿ modes (those the are most often picked as best) and selecting the modes that maximize their expected occurrence frequency given a complexity constraint. Ranking is performed by selecting a small set of macroblocks (the sampling population), for which all modes are tested. The remaining macroblocks only test the available dominant modes. The modes in the sampling population of a frame are used to process the next frame. Results are shown to verify the performance of the proposed method, which reveals sizeable complexity savings at small penalties.
Changing the Federal-University Partnership (1998)

A diamond jubilee anniversary for the Journal of Chemical Education is a time to learn from history and a time to look forward. It is also such a time for the federalâ€“university partnership. That partnership, having its antecedents in the land-grant colleges established after the Civil War, witnessed rapid growth following World War II and the launch of Sputnik, but now shows signs of weakening as each partner demands more of the other and seems to offer less. There is no question that the university system will survive and that some relationship will exist between it and the Federal government. The question is whether this relationship will be that of a functioning partnership or that of contractor and contractee. If only the latter, then we will have lost something quite precious.