gebhart/patrepeval huggingface dataset:
A New Strategy for the Improvement of Photophysical Properties in Ruthenium(II) Polypyridyl Complexes. Synthesis and Photophysical and Electrochemical Characterization of Six Mononuclear Ruthenium(II) Bisterpyridine-Type Complexes (2005)

The synthesis and characterization of six ruthenium(II) bistridentate polypyridyl complexes is described. These were designed on the basis of a new approach to increase the excited-state lifetime of ruthenium(II) bisterpyridine-type complexes. By the use of a bipyridylpyridyl methane ligand in place of terpyridine, the coordination environment of the metal ion becomes nearly octahedral and the rate of deactivation via ligand-field (i.e., metal-centered) states was reduced as shown by temperature-dependent emission lifetime studies. Still, the possibility to make quasi-linear donor−ruthenium−acceptor triads is maintained in the complexes. The most promising complex shows an excited-state lifetime of τ = 15 ns in alcohol solutions at room temperature, which should be compared to a lifetime of τ = 0.25 ns for [Ru(tpy)2]2+. The X-ray structure of the new complex indeed shows a more octahedral geometry than that of [Ru(tpy)2]2+. Most importantly, the high excited-state energy was retained, and thus, so was the potential high reactivity of the excited complex, which has not been the case with previously published strategies based on bistridentate complexes.
Measurement of stimulated-Brillouin-scattering threshold for various types of fibers using Brillouin optical-time-domain reflectometer (2000)

We have investigated the stimulated Brillouin scattering (SBS) threshold of various types of fibers both theoretically and experimentally by utilizing Brillouin optical-time-domain reflectometer. The measured deviations of SBS threshold of less than /spl plusmn/0.1 dB compared with the conventional method are demonstrated for standard single-mode fiber (SMF), large-effective-area dispersion-shifted fiber (LEAF), TrueWave/reduced slope fiber (TWF), and conventional dispersion-shifted fiber (DSF).
Biologically Active Secondary Metabolites from the Ascomycete A111-95. 1. Production, Isolation and Biological Activities. (2002)

Eight secondary metabolites were isolated from submerged cultures of the ascomycete A111-95 during a search for new nematicidal metabolites. (-)-Galiellalactone (7) and compound 2 are metabolites previously obtained from cultures of Galiella rufa while the compounds 1, 3, 4, 5, 6 and 8 (3 and 4 were obtained as an unseparable mixture), were isolated as natural products for the first time. Compound 2, pregaliellalactone (5) and the mixture of 3 and 4 showed nematicidal activities towards Caenorhabditis elegans and Meloidogyne incognita. All compounds showed moderate or weak cytotoxic activities.
Use of Confocal Laser Scanning Microscope in Cytochemistry. (1994)

The applications of a confocal laser scanning microscope for imaging fast dynamic changes of the intracellular calcium ion concentration ([Ca2+]i) in the living cell are described. Confocal images enable us to determine the cell-to-cell junction between clustered heart muscle cells of guinea pig and reveal that the high [Ca2+]i can evoke calcium-induced calcium release from the sarcoplasmic reticulum in the neighboring heart muscle cells through the gap junction. In rat peritoneal mast cells, secretagogue-induced calcium signaling initiates at the periphery of the cell and propagates to the nucleus as calcium wave. Nuclear [Ca2+]i is upheld at higher level than cytoplasmic [Ca2+]i for 2min. X-t scan mode with high-temporal resolution provides comprehensive images of the phenomenon. The confocal laser scanning microscope with depth-discriminating ability is a valuable tool to know a sequence of biological event within the living cell.
Fig. S7 from Small-Molecule–Mediated Stabilization of PP2A Modulates the Homologous Recombination Pathway and Potentiates DNA Damage-Induced Cell Death (2023)

&lt;p&gt;Fig. S7&lt;/p&gt;
A Ubiquitin-specific Protease Possesses a Decisive Role for Adenovirus Replication and Oncogene-mediated Transformation (2013)

Adenoviral replication depends on viral as well as cellular proteins. However, little is known about cellular proteins promoting adenoviral replication. In our screens to identify such proteins, we discovered a cellular component of the ubiquitin proteasome pathway interacting with the central regulator of adenoviral replication. Our binding assays mapped a specific interaction between the N-terminal domains of both viral E1B-55K and USP7, a deubiquitinating enzyme. RNA interference-mediated downregulation of USP7 severely reduced E1B-55K protein levels, but more importantly negatively affected adenoviral replication. We also succeeded in resynthesizing an inhibitor of USP7, which like the knockdown background reduced adenoviral replication. Further assays revealed that not only adenoviral growth, but also adenoviral oncogene-driven cellular transformation relies on the functions of USP7. Our data provide insights into an intricate mechanistic pathway usurped by an adenovirus to promote its replication and oncogenic functions, and at the same time open up possibilities for new antiviral strategies.
Design and Synthesis of Chiral Heteroleptic Rhodium(II) Carboxylate Catalysts: Experimental Investigation of Halogen Bond Rigidification Effects in Asymmetric Cyclopropanation (2012)

A general method for the synthesis of chiral heteroleptic rhodium(II) tetracarboxylate catalysts is reported. The chlorinated TCPT unit was found to be an efficient polarity-control group, allowing the isolation of each complex from a mixture of six possible products. This approach contributes to enlarging the scope of accessible chiral Rh(II) catalysts and allowed further study of the halogen bond rigidification effect observed in chlorinated complexes.
Qualification of soft-magnetic shielding materials used in inductive wireless power transmission systems (2015)

In inductive wireless power transmission systems often soft-magnetic shielding is used to avoid lossy eddy currents being induced in electrically conducting components like batteries or ground layers of electronic circuits. Datasheet information on such shielding materials are often limited to magnetic permeability and sometimes exemplary loss information. For designing inductive wireless power systems e.g. at variable frequency or different flux excitation, detailed loss information are of interest. Therefore, it is proposed to measure the impact of these materials on the power transmission in a standardized setup, which is closely related to the real application. Here, a configuration as described in the Qi standard for wireless charging of mobile devices published by the Wireless Power Consortium is used as reference. A Figure of Merit (FoM) is presented to qualify materials concerning both a) shielding against conducting components on the backside of the receiving coil and b) establishing a high mutual inductance of the transformer coils, resulting in higher system efficiencies. Furthermore, this paper derives that material saturation is not given in properly designed 5W applications using single material sheets of 20μm thickness.
The architecture and test structures of the XC8100 FPGA family (2002)

The XC8100 FPGA family is based on a new metal-to-metal antifuse technology and a "sea-of-gates" type architecture. Programmable interconnect elements are stacked vertically between metal layers and above the fine-grained logic cells, resulting in small die sizes and low cost. The basic cell is designed specifically for technology-independent design. Each cell can be configured to implement combinatorial, sequential, or three-state buffering functions. Other architectural features include a flexible set of high-drive buffers, output slew rate controls, and PCI-compatible I/O structures. A patented programming and test structure addresses the problem of providing for 100% post-program verification without test vectors.
Data from Sequential Loss of Tumor Vessel Pericytes and Endothelial Cells after Inhibition of Platelet-Derived Growth Factor B by Selective Aptamer AX102 (2023)

&lt;div&gt;Abstract&lt;p&gt;Inhibition of platelet derived growth factor (PDGF) can increase the efficacy of other cancer therapeutics, but the cellular mechanism is incompletely understood. We examined the cellular effects on tumor vasculature of a novel DNA oligonucleotide aptamer (AX102) that selectively binds PDGF-B. Treatment with AX102 led to progressive reduction of pericytes, identified by PDGF receptor β, NG2, desmin, or α-smooth muscle actin immunoreactivity, in Lewis lung carcinomas. The decrease ranged from 35% at 2 days, 63% at 7 days, to 85% at 28 days. Most tumor vessels that lacked pericytes at 7 days subsequently regressed. Overall tumor vascularity decreased 79% over 28 days, without a corresponding decrease in tumor size. Regression of pericytes and endothelial cells led to empty basement membrane sleeves, which were visible at 7 days, but only 54% remained at 28 days. PDGF-B inhibition had a less pronounced effect on pancreatic islet tumors in RIP-Tag2 transgenic mice, where pericytes decreased 47%, vascularity decreased 38%, and basement membrane sleeves decreased 21% over 28 days. Taken together, these findings show that inhibition of PDGF-B signaling can lead to regression of tumor vessels, but the magnitude is tumor specific and does not necessarily retard tumor growth. Loss of pericytes in tumors is an expected direct consequence of PDGF-B blockade, but reduced tumor vascularity is likely to be secondary to pericyte regression. [Cancer Res 2007;67(15):7358–67]&lt;/p&gt;&lt;/div&gt;
Novel transcription factor Satb2 interacts with matrix attachment region DNA elements in a tissue‐specific manner and demonstrates cell‐type‐dependent expression in the developing mouse CNS (2005)

Satb1 is a first cell-type-specific transcription factor of a novel type that functions as a regulator of the transcription of large chromatin domains. We identified a close homologue of Satb1, Satb2, in a cDNA subtraction screening in a search for genes controlling neural differentiation. Satb2 showed 61% amino-acid homology to Satb1. Satb2 and Satb1 expression was detected in different cell subpopulations of developing mouse CNS in a mutually exclusive manner. In the electrophoretic mobility shift assay we demonstrate that nuclear extracts from the embryonic day 18.5 mouse developing neocortex, in contrast to basal ganglia, contain a protein complex interacting with matrix attachment region DNA elements (MARs) with high affinity. Endogenous Satb2 protein is a part of this complex. In the developing neocortex Satb2 was detected largely in the superficial layers. In the developing spinal cord Satb2 expression marks a subpopulation of Lbx1-positive neurons dorsally and a subgroup of Isl1-positive neurons ventrally. In the Lbx1 mutants Satb2 expression is greatly reduced. We suggest that Satb2 may regulate differentiation of subsets of neurons at the level of higher order chromatin structure via binding to MARs.
Reprogramación transcriptómica de Lactobacillus plantarum WCFS1 en respuesta al aceite de oliva (2017)

Poster presentado al IX Congreso CyTA-CESIA, celebrado en Madrid del 16 al 19 de mayo de 2017.
Lentiviral Capsid-Mediated Streptococcus pyogenes Cas9 Ribonucleoprotein Delivery for Efficient and Safe Multiplex Genome Editing (2021)

Transient expression of the CRISPR-Cas9 machinery is desirable to reduce the risks of off-targets and immune responses. Electroporation of Cas9 ribonucleoproteins (RNPs) is the most common delivery method to achieve transient Cas9 expression. Recently, retroviral capsids have been used for delivering <i>Streptococcus pyogenes</i> Cas9 RNPs, in which Cas9 was fused to the viral proteins. The fusion strategy may cause relative low capsid assembly efficiency. We recently developed virus-like particles (VLPs) consisting of lentiviral capsid and <i>Staphylococcus aureus</i> Cas9 RNPs using the specific interactions between aptamer and aptamer-binding protein (ABP), and obtained near-normal capsid assembly efficiency. Here we test whether highly active <i>Streptococcus pyogenes</i> Cas9 (SpCas9) RNP VLPs can be generated with high efficiency by aptamer/ABP interaction. We found that by optimizing the locations and types of aptamer used for single guide RNA modification, highly active SpCas9 RNP VLPs can be generated efficiently. VLP-delivered SpCas9 generated lower off-target insertions and deletions than SpCas9 RNPs delivered by electroporation. VLPs containing Cas9 from different species and targeting multiple genes can be efficiently prepared in single-particle preparation. Multiple-target VLPs were more efficient than the combination of single-target VLPs for simultaneous targeting of multiple genes. Thus, in addition to better safety features, the Cas9 VLPs are especially suited for multiplex genome editing. In summary, our VLPs offer safe, efficient, and flexible multiplex genome editing.
Polymerization of diphenyl disulfide by the S-S bond cleavage with a Lewis acid: a novel preparation route to poly(p-phenylene sulfide) (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPolymerization of diphenyl disulfide by the S-S bond cleavage with a Lewis acid: a novel preparation route to poly(p-phenylene sulfide)Eishun Tsuchida, Kimihisa Yamamoto, Hiroyuki Nishide, Shu Yoshida, and Mitsutoshi JikeiCite this: Macromolecules 1990, 23, 8, 2101–2106Publication Date (Print):April 1, 1990Publication History Published online1 May 2002Published inissue 1 April 1990https://doi.org/10.1021/ma00210a001Request reuse permissionsArticle Views816Altmetric-Citations37LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (649 KB) Get e-Alertsclose Get e-Alerts
Key native fluorophores analysis of human breast cancer tissues using Gram–Schmidt subspace method (2014)

The native fluorescence (NFL) spectra of human cancerous and normal breast tissues were excited by a selected wavelength of 300 nm to investigate the efficacy of two key fluorophores: tryptophan and reduced nicotinamide adenine dinucleotide (NADH), as cancer biomarkers. The basis spectra of these key fluorophores' subspaces spanned by the corresponding emission spectra are obtained by the Gram-Schmidt method. A support vector machine (SVM) classifier is trained in the subspace to evaluate the sensitivity, specificity, and accuracy. This research demonstrates that the NFL spectroscopy measurements are effective to detect changes of fluorophores compositions in tissues due to the development of cancer.
ChemInform Abstract: Syn‐Selectivity in Titanium(IV) Mediated Aldol Condensation of the Silyl Ketene Acetal of S‐2‐Pyridyl Thioester with Aldehydes. (1997)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Deciphering the Agonist Binding Mechanism to the Adenosine A1 Receptor (2021)

Despite being among the most characterized G protein-coupled receptors (GPCRs), adenosine receptors (ARs) have always been a difficult target in drug design. To date, no agonist other than the natural effector and the diagnostic regadenoson has been approved for human use. Recently, the structure of the adenosine A1 receptor (A<sub>1</sub>R) was determined in the active, G<sub>i</sub> protein complexed state; this has important repercussions for structure-based drug design. Here, we employed supervised molecular dynamics simulations and mutagenesis experiments to extend the structural knowledge of the binding of selective agonists to A<sub>1</sub>R. Our results identify new residues involved in the association and dissociation pathway, they suggest the binding mode of N<sup>6</sup>-cyclopentyladenosine (CPA) related ligands, and they highlight the dramatic effect that chemical modifications can have on the overall binding mechanism, paving the way for the rational development of a structure-kinetics relationship of A<sub>1</sub>R agonists.
Diastereoselective Synthesis of Aryloxy Phosphoramidate Prodrugs of 3′-Deoxy-2′,3′-didehydrothymidine Monophosphate (2010)

The first diastereoselective synthesis of aryloxy phosphoramidate prodrugs of 3'-deoxy-2',3'-didehydrothymidine monophosphate (d4TMP) is reported. In our approach, (S)-4-isopropylthiazolidine-2-thione 1 was used as a chiral auxiliary to introduce the stereochemistry at the phosphorus atom. In the last step of the developed reaction sequence, the nucleoside analogue d4T was introduced to a stereochemically pure phosphordiamidate which led to the formation of the almost diastereomerically pure phosphoramidate prodrugs 8a-d (≥95% de). As expected, the individually prepared diastereomers of the phosphoramidate prodrugs showed significant differences in the antiviral activity. Moreover, the difference was strongly dependent on the aryl substituent attached to the phosphoramidate moiety.
The neuropathogenesis of HIV-1 infection (1994)

HIV infection in brain revolves around productive viral replication in cells of mononuclear phagocyte lineage, including brain macrophages, microglia, and multinucleated giant cells [1-4]. Together, they are the investigators for cellular and viral neurotoxic activities [5-10]. Several published reports show that viral and/or cellular products produced from HIV-1-infected macrophages injure neurons and induce glial proliferation during advancing central nervous system (CNS) infection [11-18]. These findings are supported by the apparent discrepancy between the distribution and numbers of virus-infected cells and concomitant brain tissue pathology [5, 19]. Whether these soluble factors are indirectly responsible for neuronal damage remains undefined. The identification and regulation of neurotoxins produced from HIV-infected macrophages are central to uncovering how HIV mediates CNS disease. The authors who contributed to this work represent laboratories with overlapping areas of expertise. Broad-based complementary hypotheses regarding HIV neuropathogenesis are now provided.
Efficient inscription of Bragg gratings in As_2S_3 fibers using near bandgap light (2013)

Efficient inscription of Fiber Bragg gratings (FBGs) in single-mode, thin cladding As(2)S(3) fibers is demonstrated by using near bandgap light at 532 nm. The FBGs with the reflectivity of over 80% can be induced in only 80-90 s, substantially faster than in previous reports. The dynamics of the grating growth are investigated in the photosensitivity process, showing a fast blue shift of the Bragg wavelength and then a somewhat slower red shift. The aging of the grating after fabrication is also reported, indicating a 37% decay of the grating strength.
Efficient Dielectric Reflectors for Solid-state Emitters in Bulk Diamond (2017)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text N. Wan, S. Mouradian, B. Lienhard, D. Kim, M. Walsh, T. Schröder, B. Shields, and D. Englund, "Efficient Dielectric Reflectors for Solid-state Emitters in Bulk Diamond," in Conference on Lasers and Electro-Optics, OSA Technical Digest (online) (Optica Publishing Group, 2017), paper FTu1E.5. Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
Potential Impact of 68Ga-PSMA-11 PET/CT on the Planning of Definitive Radiation Therapy for Prostate Cancer (2018)

Standard-of-care imaging for initial staging of prostate cancer (PCa) underestimates disease burden. Prostate-specific membrane antigen (PSMA) PET/CT detects PCa metastasis with superior accuracy, having a potential impact on the planning of definitive radiation therapy (RT) for nonmetastatic PCa. Our objectives were to determine how often definitive RT planning based on standard target volumes covers <sup>68</sup>Ga-PSMA-11 PET/CT-defined disease and to assess the potential impact of <sup>68</sup>Ga-PSMA-11 PET/CT on definitive RT planning. <b>Methods:</b> This was a post hoc analysis of an intention-to-treat population of 73 patients with localized PCa without prior local therapy who underwent <sup>68</sup>Ga-PSMA PET/CT for initial staging as part of an investigational new drug trial. Eleven of the 73 were intermediate-risk (15%), 33 were high-risk (45%), 22 were very-high-risk (30%), and 7 were N1 (9.5%). Clinical target volumes (CTVs), which included the prostate, seminal vesicles, and (in accord with the Radiation Therapy Oncology Group consensus guidelines) pelvic lymph nodes (LNs), were contoured on the CT portion of the PET/CT images by a radiation oncologist masked to the PET findings. <sup>68</sup>Ga-PSMA-11 PET/CT images were analyzed by a nuclear medicine physician. <sup>68</sup>Ga-PSMA-11-positive lesions not covered by planning volumes based on the CTVs were considered to have a major potential impact on treatment planning. <b>Results:</b> All patients had one or more <sup>68</sup>Ga-PSMA-11-positive primary prostate lesions. Twenty-five (34%) and 7 (9.5%) of the 73 patients had <sup>68</sup>Ga-PSMA-11-positive pelvic LN and distant metastases, respectively. The sites of LN metastases in decreasing order of frequency were external iliac (20.5%), common iliac (13.5%), internal iliac (12.5%) obturator (12.5%), perirectal (4%), abdominal (4%), upper diaphragm (4%), and presacral (1.5%). The median size of the LN lesions was 6 mm (range, 4-24 mm). RT planning based on the CTVs covered 69 (94.5%) of the 73 primary lesions and 20 (80%) of the 25 pelvic LN lesions, on a per-patient analysis. <b>Conclusion:</b><sup>68</sup>Ga-PSMA-11 PET/CT had a major impact on intended definitive RT planning for PCa in 12 (16.5%) of the 73 patients whose RT fields covered the prostate, seminal vesicles, and pelvic LNs and in 25 (37%) of the 66 patients whose RT fields covered the prostate and seminal vesicles but not the pelvic LNs.
Understanding Li-Ion Battery Anode Degradation and Pore Morphological Changes through Nano-Resolution X-ray Computed Tomography (2016)

Sophisticated electrode design for high power and energy density batteries will require a more thorough understanding of how electrode porosity affects cell failure mechanisms such as solid electrolyte interphase (SEI) formation. This study uses high resolution three dimensional (3D) mapping to examine the anode SEI deposits in off the shelf Li-ion cells that have been deeply cycled to complete failure. Scanning electron microscopy (SEM) was used to show the buildup of SEI on the surface of the graphite anode, while nano-scale resolution X-ray computed tomography (nano-CT) was used to show the SEI accumulation within the anode's pore structure. Through a combination of phase contrast and absorption contrast imaging, it was shown that the average pore diameter and total internal pore volume fraction decreased significantly after cycling, while there was a corresponding increase of absorption contrast from heavier elements throughout the anode. We propose this is due to buildup of electrolyte decomposition products within the pores.