gebhart/patrepeval huggingface dataset:
Dehydro-α-lapachone, a plant product with antivascular activity (2011)

Antivascular agents have become a standard of treatment for many malignancies. However, most of them target the VEGF pathway and lead to refractoriness. To improve the diversity of options for antivascular therapy, we applied a high-throughput screen for small molecules targeting cell adhesion. We then assayed the resulting antiadhesion hits in a transgenic zebrafish line with endothelial expression of EGFP (Tg(fli1:EGFP)(y1)) to identify nontoxic molecules with antivascular activity selective to neovasculature. This screen identified dehydro-α-lapachone (DAL), a natural plant product. We found that DAL inhibits vessel regeneration, interferes with vessel anastomosis, and limits plexus formation in zebrafish. Furthermore, DAL induces vascular pruning and growth delay in orthotopic mammary tumors in mice. We show that DAL targets cell adhesion by promoting ubiquitination of the Rho-GTPase Rac1, which is frequently up-regulated in many different cancers.
Laser Direct Write Synthesis of Lead Halide Perovskites (2016)

Lead halide perovskites are increasingly considered for applications beyond photovoltaics, for example, light emission and detection, where an ability to pattern and prototype microscale geometries can facilitate the incorporation of this class of materials into devices. Here we demonstrate laser direct write of lead halide perovskites, a remarkably simple procedure that takes advantage of the inverse dependence between perovskite solubility and temperature by using a laser to induce localized heating of an absorbing substrate. We demonstrate arbitrary pattern formation of crystalline CH<sub>3</sub>NH<sub>3</sub>PbBr<sub>3</sub> on a range of substrates and fabricate and characterize a microscale photodetector using this approach. This direct write methodology provides a path forward for the prototyping and production of perovskite-based devices.
Comparison of Two Assay Techniques to Study In Vitro Phagocytosis of Bovine Granulocytes Using Flow Cytometry (2000)

The response of phagocytic cells to Pasteurella haemolytica is difficult to study in situ. Available assays to study the function of these cell types often do not accurately reflect the physiology of the cell in response to bovine pasteurellosis. This study compared granulocyte phagocytosis of fluorescent beads and fluorescent bacteria using flow cytometry. Development of an in vitro assay would provide a model to study nutrient and pharmaceutical effects on the immune response to Pasteurella infections. It would also allow for future studies of the pathogenesis of not only bovine pasteurellosis but other diseases.
A Case of Cystic Variant of Mucus-producing Adenocarcinoma of the Lung. (1991)

症例は73歳, 重喫煙者の男性 (BI: 3180). 他疾患で入院時, 胸部X線で左肺S9に辺縁明瞭な分葉を有する腫瘤陰影を指摘され当院に紹介された. 擦過細胞診および穿刺細胞診で確診を得られなかったが, 陰影の増大を認めたため左下葉切除術を行った. 腫瘤は4.5cm径, 柔らかく, 辺縁は明瞭, 内部は粘液を大量に含み嚢胞状を呈していた. 病理組織学的に腫瘤内部の大半は粘液からなり, 杯細胞型癌細胞が腫瘍の辺縁部と肺胞壁にわずかに観察された. 本例を肺粘液癌 (p-T2N0M0) と診断したが, 興味深い臨床経過と嚢胞状に拡張した特異な病理形態呈したことから肺粘液産生腺癌の嚢胞状亜型と考えた.
Four-level medium voltage multilevel converter for high power applications (2013)

This work presents a preliminary analysis on a new 4-level medium voltage multilevel converter topology. The topology is able to reach medium-voltage operation for 3.3 kV and 6.6 kV applications with conventional semiconductors such as IGCTs or HV-IGBTs. The switching states and their equivalent circuits are presented, along with an adapted multi-level modulation scheme based on level shifted PWM. As with other multilevel converter topologies a dc-link voltage balance algorithm is required, which is also introduced in this paper. Simulation results feeding an RL load are included to to give a first glance on performance of the converter. The main advantages and disadvantages of the proposed topology are also addressed, including future work.
Regional variability of prostacyclin biosynthesis. (1989)

To investigate the regional variability in arterial and venous endothelial prostacyclin (PGI2) biosynthesis, we obtained 1-cm segments of carotid arteries, external jugular veins, femoral arteries and veins, iliac arteries and veins, inferior venae cavae (IVC), and aortas from 17 dogs. Vessel luminal PGI2 production was measured in the basal state by radioimmunoassay of 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha). A total of 90 arterial specimens (57, 19, and 14 segments, respectively, of femoral/carotid arteries, iliac arteries, and aorta) and 41 venous specimens (15, 10, and 16 segments, respectively, of femoral/jugular veins, iliac veins, and IVC) were analyzed. Overall, arterial endothelial 6-keto-PGF1 alpha was higher than venous (8.1 +/- 0.5 ng/ml vs. 4.9 +/- 0.7 ng/ml, p less than 0.0004); 6-keto-PGF1 alpha levels were greater in the arteries than in their corresponding veins [femoral/carotid arteries (6.3 +/- 0.4 ng/ml) vs. femoral/jugular vein (2.1 +/- 0.4 ng/ml), p less than 0.0002; iliac arteries (9.3 +/- 1.0 ng/ml) vs. iliac veins (4.8 +/- 0.9 ng/ml), p less than 0.005; aorta (14.0 +/- 1.6 ng/ml) vs. IVC (7.5 +/- 1.4 ng/ml), p less than 0.006].(ABSTRACT TRUNCATED AT 250 WORDS)
Transient fuse structures: The role of metal etching vs. dielectric deposition (2002)

Transient fuse (or T-fuse) structures have been used in recent years to study charging from metal etching. However, we identify here a case where the charging detected by the T-fuse came not from metal overetching but from the subsequent dielectric deposition step. This HDP CVD oxide deposition process caused charging through metal finger antennas that appears much like metal overetch-induced charging, but experiments varying etch process parameters or omitting the deposition step showed that deposition was the source of charging. Presumably the charging occurred early in the deposition process, when the metal antennas were exposed to the deposition plasma. The space width dependence of charging had a signature unlike that expected from electron shading during metal etching: charging diminished rather than increased as space width narrowed.
Downtilted Base Station Antennas - A Simulation Model Proposal and Impact on HSPA and LTE Performance (2008)

This paper proposes a low-complexity model for vertical antenna radiation patterns, e.g. for inclusion in system- level simulations. They can be seen as extensions to the horizontal radiation pattern model used in 3GPP simulation scenarios. The model is verified against and compared to predicted and measured data from real networks. The impact on system-level performance is also investigated. It is seen that using the proposed model, simulated geometry distributions and soft handover statistics closely matching those of real networks may be achieved. The analysis also concludes that many real networks have better cell isolation than what is modeled by the 3GPP antenna model. As a consequence, the horizontal radiation pattern model significantly under-estimates the system level performance in such networks. Furthermore, the proposed model is used to assess the LTE and HSPA system-level performance for realistic scenarios.
Gene transfer from engineered Lactococcus lactis strains to Enterococcus faecalis in the digestive tract of gnotobiotic mice. (1993)

The introduction of genetically modified organisms into food products requires an evaluation of the behaviour and the dissemination of foreign genes of such organisms among the human intestinal microflora. The conjugal transfer, both in vitro and in vivo (in mice digestive tract) of DNA from Lactococcus lactis donor strains to an Enterococcus faecalis strain isolated from human faecal flora was studied. We followed the transfer of (1) the self-transmissible plasmid pIL205; (2) two non-self-transmissible but mobilizable plasmids, pIL252 and pIL253; (3) one plasmid, pMS1.5B, integrated into the chromosome of L. lactis. In vitro, the transfer frequency of pIL205 (expressed as the number of transconjugants per donor cell) was 9.6 x 10(-4); mobilization of one of the non-self-transmissible plasmids, pIL253, was observed (4.9 x 10(-7)). In vivo, only transfer of pIL205 and pIL253 occurred, but the frequency was not determined. The transfer of pMS1.5B was not detected in vitro or in vivo.
3D hand shape analysis for palm and fingers identification (2015)

This paper proposes a novel scheme for the extraction and identification of the palm and the fingers from a single depth map. The hand is firstly segmented from the rest of the scene, then it is divided into palm and fingers regions. For this task we employed a novel scheme that exploits the idea that fingers have a tubular shape while the palm is more planar. Following this rationale we applied a contraction guided by the normals in order to reduce the fingers into thinner structures that can be identified by analyzing the changes in the point density. Density-based clustering is then applied and finally a linear programming based approach is employed to identify the various fingers. Experimental results prove the effectiveness of the proposed approach even in complex situations and in presence of inter-occlusions between the various fingers.
Role of Plasmin and uPAR in Bone Marrow HSC/HPC Retention and Mobilization. (2005)

Abstract We previously identified the plasmin protease family as a critical determinant of the mobilization of hematopoietic stem and progenitor cells (HSC/HPC), but the role of the urokinase receptor uPAR remained unclear. uPAR is a membrane-anchored glycoprotein, which not only localizes its ligand urokinase (uPA) to the cell surface via its GPI-anchor but also regulates β1-integrin dependent cell adhesion and migration. Following 5-FU myeloablation or G-CSF treatment, mice lacking uPAR (uPAR−/−) had impaired hematopoietic recovery and HSC/HPC mobilization as compared to wild type (WT) mice. However, this phenotype was not mimicked in mice lacking uPA, suggesting a role of uPAR in mobilization independent of uPA-mediated proteolysis. The impaired mobilization in uPAR−/− mice was reversed upon pre-transplantation with WT BM cells (BMC), suggesting functional expression of uPAR on transplantable BMCs. Conversely, loss or inhibition of uPAR on transplanted BMCs impaired homing to the BM but not to the spleen, and compromised survival of myeloablated WT recipients. In vitro experiments revealed that loss or inhibition of uPAR impaired BMC adhesion to stromal cells and fibronectin. Anti-α4-β1 antibodies blocked adhesion of WT but not uPAR−/− BMCs. Thus, uPAR appears to regulate BM homing and α4-β1 dependent retention of transplantable BMCs, possibly HSC/HPCs. If uPAR mediates retention of HSC/HPCs, then this signal should be inactivated upon mobilization. Indeed, in 5-FU or G-CSF-treated WT mice, we found increased uPAR cleavage, and elevated levels of soluble uPAR (suPAR) in BM plasma. These processes failed to occur in mice lacking plasminogen, suggesting that plasmin cleaves uPAR during mobilization. Cleavage of uPAR appeared critical as the inactivation of the retention signals membrane-bound Kit ligand and SDF-1α was normal in uPAR−/− mice. Moreover, the generated suPAR may also affect the BM, as administration of recombinant suPAR in WT mice enhanced hematopoietic recovery and HSC/HPC mobilization after 5-FU or G-CSF. In vitro and transplantation experiments revealed that suPAR blocked α4-β1 dependent adhesion. Thus, in steady state, membrane-anchored uPAR appears to function as a BM retention signal for transplantable BMCs, possibly HSC/HPCs. In conditions of mobilization, the uPAR retention signal is cleaved, which weakens α4-β1 dependent adhesion and allows mobilization out of the BM. Soluble uPAR may then additionally amplify mobilization, in part by further attenuating α4-β1 dependent adhesion to the BM. Currently, we are investigating the role of uPAR on subsets of HSC/HPCs, and in the different BM niches. We are also performing long-term competitive repopulation experiments to further delineate the therapeutic potential of uPAR.
The Importance of Be(ginn)Ing Naive: Implications for Cancer Immune-Gene Therapy (2011)

Abstract Abstract 2052 T cell engineering against tumor antigens aims at ameliorating current immunotherapeutic strategies. To date, however, the suboptimal persistence of the transferred cells represents a serious limitation of this approach. The most appropriate T cell subset to be infused should ensure optimal in vivo persistence and yet appropriate anti-tumor activity. Here we report that culturing highly purified naïve T (TN) cells with beads conjugated to anti-CD3 and anti-CD28 antibodies, allows the retrieval of a novel post-mitotic CD45RA+ CD62L+ CCR7+ T cell population, which requires IL-7 and IL-15 for expansion and maintenance. This population is highly proliferative and sensitive to RV and LV transduction, expresses low levels of γIFN and cytotoxic molecules and is best defined as IL-7Rα+ CXCR4+ c-kit+ CCR5− HLA-DR− PD-1−. When infused in immunodeficient mice, genetically manipulated and in vitro expanded TN proved superior engraftment and longer persistence than transduced central memory (TCM) cells, and xenoreactivity comparable to that of unmanipulated lymphocytes. Engineered TN, but not TCM, maintained engraftment and xenoreactivity in serial transplantation experiments, indicating unique self-renewal abilities. Given the great potentials of this novel TN-derived cell population for immune-gene therapy, we further characterized it by molecular profiling. The gene expression signature is typical of antigen-experienced lymphocytes and classifies these cells between naturally occurring TN and TCM lymphocytes. Because of this and of the self-renewal abilities displayed in vivo, we termed them as precursor to TCM (TpreCM). We next sought to identify the natural counterpart of this TpreCM population in healthy donors, exploiting some of the markers present in the TpreCM signature, such as CD95 which is expressed by all memory T subsets but not by TN, and we selected the pp65 protein of cytomegalovirus (CMV) as a model antigen. CMV persistent infection induces a T-cell response that is maintained throughout life, indicating that a self-renewing memory T cells are generated. We studied the phenotype of CMV pp65-specific CD8+ T cells in seropositive donors and identified antigen-specific CD45RA+CD62L+. Among CD45RA+CD62L+ cells CMV-specific cells were enriched for CD45ROdim and CD95+ lymphocytes, which represent bona fide TpreCM. To functionally characterize natural TpreCM, we sorted CD45RA+CD62L+ cells according to their CD95 expression and challenged them with increasing doses anti-CD3 antibody, with or without a costimulatory signal. We found that among CD45RA+CD62L+ cells, only CD95+ lymphocytes were responsive to TCR-triggering alone, while CD95− cells required costimulation to proliferate. In conclusion, we identified a novel memory T cell subset, and identified conditions able to gene-modify and expand these memory lymphocytes while preserving their functional characteristics. Exploitation of these concepts might improve cancer adoptive immunotherapy. Disclosures: Bonini: MolMed: Consultancy.
Contrasting Behavior of Conformationally Locked Carbocyclic Nucleosides of Adenosine and Cytidine as Substrates for Deaminases (2020)

In addition to the already known differences between adenosine deaminase (ADA) and cytidine deaminase (CDA) in terms of their tertiary structure, the sphere of Zn+2 coordination, and their reverse stereochemical preference, we present evidence that the enzymes also differ significantly in terms of the North/South conformational preferences for their substrates and the extent to which the lack of the O(4’) oxygen affects the kinetics of the enzymatic deamination of carbocyclic substrates. The carbocyclic nucleoside substrates used in this study have either a flexible cyclopentane ring or a rigid bicyclo[3.1.0]hexane scaffold.
Multi-level 3-D rotational invariant classification (2003)

A two-level 3D rotational invariant classification is developed based on fractional differencing model. In first level, classification has been done with a fractal scale, and in second level, textures have been classified further in detail with the additional frequency parameters. Because of the properties of the fractal scale and multi-level procedure, the proposed 3D rotational invariant classification scheme reduces the processing time and gives enough accuracy of the classification simultaneously. As a result of a series of experiments involving the differently oriented samples of natural textures, it is concluded that these combined features make possible for this multi-level classification method to have a strong class separability power for arbitrary oriented 3D texture patterns.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
The chromosomal virulence gene, chvE, of Agrobacterium tumefaciens is regulated by a LysR family member (1993)

Certain plant phenolic compounds and monosaccharides induce the transcription of virulence (vir) genes of Agrobacterium tumefaciens through the VirA-VirG two-component regulatory system. The product of the chromosomal virulence gene chvE is homologous to galactose-binding protein of Escherichia coli and is required for vir gene induction by sugars. Adjacent to, but divergent in transcription from, chvE is an open reading frame, now termed gbpR (galactose-binding protein regulator), that is homologous to the LysR family of transcriptional regulators. chvE::lacZ expression was induced by L-arabinose, D-galactose, and D-fucose when gbpR was present. In the absence of inducer, GbpR repressed chvE::lacZ expression. In addition, GbpR negatively regulated its own expression.
Real‐time depth enhancement by fusion for RGB‐D cameras (2013)

This study presents a real‐time refinement procedure for depth data acquired by RGB‐D cameras. Data from RGB‐D cameras suffer from undesired artefacts such as edge inaccuracies or holes owing to occlusions or low object remission. In this work, the authors use recent depth enhancement filters intended for time‐of‐flight cameras, and extend them to structured light‐based depth cameras, such as the Kinect camera. Thus, given a depth map and its corresponding two‐dimensional image, we correct the depth measurements by separately treating its undesired regions. To that end, the authors propose specific confidence maps to tackle areas in the scene that require a special treatment. Furthermore, in the case of filtering artefacts, the authors introduce the use of RGB images as guidance images as an alternative to real‐time state‐of‐the‐art fusion filters that use greyscale guidance images. The experimental results show that the proposed fusion filter provides dense depth maps with corrected erroneous or invalid depth measurements and adjusted depth edges. In addition, the authors propose a mathematical formulation that enables to use the filter in real‐time applications.
Supplementary Movie 4 from The Anaplastic Lymphoma Kinase Controls Cell Shape and Growth of Anaplastic Large Cell Lymphoma through Cdc42 Activation (2023)

Supplementary Movie 4 from The Anaplastic Lymphoma Kinase Controls Cell Shape and Growth of Anaplastic Large Cell Lymphoma through Cdc42 Activation
First clinical trial of a newly developed capsule endoscope with panoramic side view for small bowel: A pilot study (2013)

CapsoCam SV1 is a safe and efficient tool in small bowel examination. The duodenal papilla as the only landmark in small bowel is detected in more than 70% of the patients.
A 20K-gate CMOS gate array (1983)

Combining an advanced 2-/spl mu/m CMOS technology with a newly developed triple-level metallization technology, a high-performance 20K-gate CMOS gate array has been developed. The advantage of triple-level metallization for area saving in a large-scale gate array was evaluated by a computer simulation. The typical gate delay is 1.5 ns with fan-out of 3, and 3-mm metal interconnect length. As a test vehicle for verifying the high-performance gate array, a 32/spl times/32-bit parallel multiplier has been successfully designed and fabricated. Cell utilization is about 65%. A typical multiplication takes 120 ns at a 5-MHz clock rate, with a power dissipation of 400 mW.
Estimating Chemical Doses for Water Stabilization (1987)

A simple method for directly estimating the lime and carbon dioxide doses needed to produce water that is stable with respect to calcium carbonate has been developed. The procedures employed are readily applicable using either a straightforward graphical approach or a user‐friendly microcomputer program. The method is superior to Caldwell‐Lawrence diagrams and the various stability indices that have been proposed in that chemical doses are obtained directly, without a trial‐and‐error approach. The procedures are based on numerically solving the equations governing calcium carbonate (calcite) equilibrium. The only data required are the calcium hardness, alkalinity, pH, temperature, and total dissolved solids (or ionic strength) of the water to be treated.
Long-term antigen retention by dendritic cells in the popliteal lymph node of immunized mice. (1981)

Antigen retention by follicular dendritic cells (FDC) was studied in the popliteal lymph nodes (PLN) of mice actively or passively immunized against human serum albumin (HSA) or horse spleen ferritin. Electron microscopic autoradiography was used to locate a challenge dose (1 microgram) of 125I-labelled HSA in the draining PLN following injection into the hind footpad of specifically immune mice. In both actively and passively immunized mice, the radiolabelled antigen was localized to the follicles in the cortex of the draining node. In actively immunized mice, the antigen formed a crescent of label on the superficial aspect of germinal centres, while in passively immunized mice label was seen in primary follicles. In electron microscope autoradiographs, the silver grains were concentrated in areas of dendritic cell processes which emanated from a cell body containing a characteristic irregular nucleus. The size and complexity of the dendritic cell processes increased in actively immunized mice suggesting that the FDC could hypertrophy. High resolution studies using the electron-dense antigen, ferritin, showed that it was localized to the extracellular space of the FDC processes and was associated with amorphous electron-dense material; presumably immune complexes. Antigen was not present uniformly distributed in the extracellular material but rather it was in discrete patches occupying small segments of the FDC processes. Large amounts of label-free electron-dense material were present suggesting that immune complexes of various specificities were present on each DC. Ferritin was seen more than 3 months after challenge but only on the FDC. The data suggest that the antigen retaining mechanism in the lymph node of immune mice is antigen non-specific, is capable of hypertrophy in response to active immunization and provides a mechanism for stable long-term retention of antigen.