gebhart/patrepeval huggingface dataset:
Lysostaphin Lysibody Leads to Effective Opsonization and Killing of Methicillin-Resistant Staphylococcus aureus in a Murine Model (2018)

The cell wall of Gram-positive bacteria contains abundant surface-exposed carbohydrate structures that are highly conserved. While these properties make surface carbohydrates ideal targets for immunotherapy, carbohydrates elicit a poor immune response that results primarily in low-affinity IgM antibodies. In a previous publication, we introduced the lysibody approach to address this shortcoming. Lysibodies are engineered molecules that combine a high-affinity carbohydrate-binding domain of bacterial or bacteriophage origin and an Fc effector portion of a human IgG antibody, thus directing effective immunity to conserved bacterial surface carbohydrates. Here, we describe the first example of a lysibody containing the binding domain from a bacteriocin, lysostaphin. We also describe the creation of five lysibodies with binding domains derived from phage lysins, directed against <i>Staphylococcus aureus</i> The lysostaphin and LysK lysibodies showed the most promise and were further characterized. Both lysibodies bound a range of clinically important staphylococcal strains, fixed complement on the staphylococcal surface, and induced phagocytosis of <i>S. aureus</i> by macrophages and human neutrophils. The lysostaphin lysibody had superior <i>in vitro</i> activity compared to that of the LysK lysibody, as well as that of the previously characterized ClyS lysibody, and it effectively protected mice in a kidney abscess/bacteremia model. These results further demonstrate that the lysibody approach is a reproducible means of creating antibacterial antibodies that cannot be produced by conventional means. Lysibodies therefore are a promising solution for opsonic antibodies that may be used passively to both treat and prevent infection by drug-resistant pathogens.
Accuracy of Glenoid Component Placement in Total Shoulder Arthroplasty and Its Effect on Clinical and Radiological Outcome in a Retrospective, Longitudinal, Monocentric Open Study (2013)

this study demonstrates that glenoid implants of anatomic TSA are poorly positioned and that this malposition has a direct effect on the clinical and radiological outcome. Thus, further developments in glenoid implantation techniques are required to enable the surgeon to achieve a desired implant position and outcome.
A dynamic stream link for efficient data flow control in NoC based heterogeneous MPSoC (2013)

As Systems-on-Chip size increase, the communication costs become critical and Networks-on-Chip (NoC) bring innovative solutions. Efficient stream-based protocols over NoC have been widely studied to address dataflow communications. They are usually controlled by a set of static parameters. However, new applications, such as high-resolution video decoders, present more data-dependent behaviors forcing communication protocols to support higher dynamicity. For this purpose, we present in this paper dynamic stream links for stream-based end-to-end NoC communications by introducing two link protocols, both independent of the transfer size, allowing to improve the hardware/software control flexibility. The proposed protocols have been modeled in a MPSoC virtual platform and the hardware cost evaluated. Based on simulations, we provide guidelines to exploit these protocols according to application needs.
Design of Passive Ultra-High Frequency Radio-Frequency Identification Tag (2008)

International audience
Sodium superionic conduction in Na₂B₁₂H₁₂ (2014)

Impedance measurements indicate that Na₂B₁₂H₁₂ exhibits dramatic Na⁺ conductivity (on the order of 0.1 S cm⁻¹) above its order-disorder phase-transition at ≈529 K, rivaling that of current, solid-state, ceramic-based, Na-battery electrolytes. Superionicity may be aided by the large size, quasispherical shape, and high rotational mobility of the B₁₂H₁₂²⁻ anions.
The mixotope: a combinatorial peptide library as a T cell and B cell immunogen (1994)

We report a new approach in peptide vaccine strategy based on combinatorial synthesis. A library of 7.5 x 10(5) related peptides, termed mixotope, was derived from the sequence of the third hypervariable domain (V3 loop) of the human immunodeficiency virus (HIV) envelope protein. This preparation induced a strong immune response in all syngeneic and outbred rodents tested. The response directed against the mixotope included antibodies, CD4+ T helper cells (TH1 and TH2) and CD8+ T cells. In rodents immunized with the mixotope, the T cell response directed against individual V3 peptide sequences (BRU, MN, RF, SF2, and ELI) as measured by T cell proliferation and interleukin (IL)-2 production, was found to be major histocompatibility complex haplotype-dependent. However, additional experiments performed in mice indicated that selectivity was less restrictive when using IL-3 secretion to explore T cell activation. This combinatorial antigen could be considered as a series of agretopic motifs framing a multiplicity of closely related epitopes for T cell recognition and able to elicit a T cell and B cell repertoire. This new construct may therefore provide a basis for the design of future vaccine strategies.
Methods of pig identification (Revised 1976) (1976)

Various methods of pig identification are available for swine producers.Ear tags may be placed in the ear for temporary identification.Ear tags of various colors, number color codes, and manufacturer types can be used for quick and easy identification.Place tags on the top side of the ear on drooping-eared breeds and the inside of the ear on erect-eared breeds.Various commercial companies, such as Fearing, Lone Star, Modern Farm, and Ritchey, produce plastic tags which are pliable, color coded, manually color coded, factory numbered or coded, and easily inserted or attached.
Acousto-optic scanning and interfering photon density waves for precise localization of an absorbing (or fluorescent) body in a turbid medium (1993)

In most optical methods proposed for imaging an absorbing object embedded in a turbid medium, data are collected using a single source and detector scanned mechanically across the surface of the medium. In our setup, we exploited destructive interference of diffusive photon density waves originating from two sources to localize an absorbing (or fluorescent) body in a scattering medium. A frequency-domain instrumentation is described that scans several laser-beam spots across the surface of a turbid medium using 1D (or 2D) acousto-optical deflectors. An additional acousto-optic deflector is used to establish arbitrary phase shifts for the interfering photon-density waves. A destructive interference pattern was created to laterally localize an absorbing (or fluorescent) body in the reflection and transmission modes. In some experiments the destructive interference pattern was altered by modulating the individual beam intensities to improve sensitivity and ameliorate surface texture problems. The experimental results were retrieved from a gated intensified CCD camera at 246 MHz modulation frequency. Results indicate that less than a 1 mm displacement of a small object embedded 10 mm in a medium with optical characteristics similar to bloodless skin tissue can be detected.
Diagnosis of female 17α-hydroxylase deficiency after gonadectomy: a case report (2019)

The differential diagnosis of disorders of sex development can be difficult at a young age without complete expression of the phenotype. However, diagnosis at a later age would change the treatment and prognosis of the disease; therefore, a genetic examination should be considered.
Biomedical Applications of Nanotechnology (2007)

Preface. Contributors. 1. Biological Applications of Multifunctional Magnetic Nanowires. 2. Nucleic Acid Delivery and Localizing Delivery with Magnetic Nanoparticles. 3. Magnetic Nanoparticles in Cancer Diagnosis and Hyperthermic Treatment. 4. Brownian Motion In Biological Sensing. 5. Dendrimers And Hyperbranched Polymers For Drug Delivery. 6. Nanogels: Chemistry to Drug Delivery. 7. Targeted Gold Nanoparticles for Imaging and Therapy. 8. Building Blocks of Nucleic Acid Nanostructures: Unfolding Thermodynamics of Intramolecular DNA Complexes. 9. Nanotoxicology. Index. About the Editors.
Two-color interferometer for study of dense low-ionized plasma on the target in high-power pulse linear accelerator (2021)

A two-color infrared interferometer has been developed for the investigation of high-density weakly ionized tantalum plasma in x-ray complexes based on linear induction accelerators (1.6 kA electron beam current, 4.6 MeV energy, and 100 ns pulse duration). Simultaneous probing at two different wavelengths makes it possible to independently measure the density of neutral and electron components. The interferometer uses wavelength values of 1.064 µm (Nd:YAG laser) and 10.6 µm (CO<sub>2</sub> laser). To attenuate the effect of sample beam refraction in inhomogeneous plasma, the interferometer used a refraction suppression scheme composed of spherical mirrors focusing the object beam into the region occupied by the plasma. In addition, the power of the sample beam transmitted through the plasma was controlled in order to analyze whether there was a distortion of the interference pattern because of strong sample beam refraction and absorption in the plasma cloud. To calibrate the initial phase shift of the probe radiation, a movable mirror mounted on a piezoelectric element and oscillating according to a harmonic law with amplitude greater than the laser wavelengths was used. In initial experiments, the parameters of target plasma registered by this interferometer are as follows: the linear density of neutrals reached 1.5 · 10<sup>17</sup> cm<sup>-2</sup>, and the degree of ionization was of the order of 10<sup>-2</sup>. The target plasma expansion velocity is determined as ∼6 km/s.
Homogeneous production and removal of NO/sub x/ from combustion exhaust gases. Seventh quarterly technical summary report, 1 March-31 May 1980 (1980)

The rate constants for the reaction OH + NH/sub 3/ ..-->.. NH/sub 2/ + H/sub 2/O have been measured over the temperature range 294 - 1075 K. The data can be fit by the Arrhenius expression k(cm/sup 3/ molecule/sup -1/ s/sup -1/) = 5.41 +- 0.86 x 10/sup -12/ exp(-2120 +- 143 kcal mol/sup -1//RT). A chemical model of the NO/sub x/ chemistry has been developed which includes 54 reactions. Although not yet complete, the reaction set clearly indicates the key reaction is NH/sub 2/ + NO. The determination of the product channels and their branching ratios is of utmost importance in understanding the effect of NH/sub 3/ addition to combustor exhaust streams for removal of NO/sub x/.
Figure S2 from Tumor-Specific Inhibition of <i>In Situ</i> Vaccination by Distant Untreated Tumor Sites (2023)

&lt;p&gt;Cooperative effect of RT and IT-hu14.18-IL2 is observed in mice bearing a single syngeneic Panc02-GD2+ tumor.&lt;/p&gt;
Paper #250: Effect of Angiogenesis on the Regenerative Capacity of ACL‐derived CD34+ Cells in ACL Reconstruction (2013)

This study indicates the transplanted ACL-derived CD34+ cells with VEGF (25%) increased neo-angiogenesis, bone-tendon healing and biomechanical strength. On the other hand, blocking angiogenesis with sFLT1 significantly decreased biomechanical strength and excessive over-expression of VEGF showed no significant difference compared to the control groups. Further studies will provide novel insights.
MEPE loss-of-function variant associates with decreased bone mineral density and increased fracture risk (2020)

A major challenge in genetic association studies is that most associated variants fall in the non-coding part of the human genome. We searched for variants associated with bone mineral density (BMD) after enriching the discovery cohort for loss-of-function (LoF) mutations by sequencing a subset of the Nord-Trøndelag Health Study, followed by imputation in the remaining sample (N = 19,705), and identified ten known BMD loci. However, one previously unreported variant, LoF mutation in MEPE, p.(Lys70IlefsTer26, minor allele frequency [MAF] = 0.8%), was associated with decreased ultradistal forearm BMD (P-value = 2.1 × 10<sup>-18</sup>), and increased osteoporosis (P-value = 4.2 × 10<sup>-5</sup>) and fracture risk (P-value = 1.6 × 10<sup>-5</sup>). The MEPE LoF association with BMD and fractures was further evaluated in 279,435 UK (MAF = 0.05%, heel bone estimated BMD P-value = 1.2 × 10<sup>-16</sup>, any fracture P-value = 0.05) and 375,984 Icelandic samples (MAF = 0.03%, arm BMD P-value = 0.12, forearm fracture P-value = 0.005). Screening for the MEPE LoF mutations before adulthood could potentially prevent osteoporosis and fractures due to the lifelong effect on BMD observed in the study. A key implication for precision medicine is that high-impact functional variants missing from the publicly available cosmopolitan panels could be clinically more relevant than polygenic risk scores.
Verminderte Glyoxalase-1 Aktivität in Patienten mit schmerzhaften diabetischen Neuropathie (2007)

Ziele: Die Akkumulation von Advanced Glycation Endproducts (AGEs) im Gewebe von Patienten mit Diabetes mellitus (DM) gilt als eine mögliche Ursache für die Entstehung diabetischer Spätschäden. Da zahlreiche AGEs aus Dicarbonylverbindungen wie Methylglyoxal (MG) gebildet werden können, stellt das intrazelluläre Glyoxalasesystem, das MG und andere α-Oxoaldehyde detoxifizieren kann, einen wichtigen Abwehrmechanismus dar. In eigenen Vorarbeiten konnten wir nachweisen, dass Hyperglykämie die Glyoxalase-1 (GO-1)- Transkription, Expression und Aktivität in vitro und in experimentellen Diabetes-Modellen mindert und das Schmerzempfinden insbesondere in frühen Phasen der diabetischen Neuropathie steigert. Daher untersuchten wir das Ausmaß der GO-1-Aktivität in Patienten mit Diabetes Typ-1 und Typ-2 und schmerzhafter diabetischer Polyeuropathie.
Characterization of a recombinant soluble form of human placental leucine aminopeptidase/oxytocinase expressed in Chinese hamster ovary cells (2000)

Serum levels of human placental leucine aminopeptidase/oxytocinase (P-LAP) increase with gestation. cDNA cloning of P-LAP revealed that the enzyme is a type II membrane-bound protein containing the consensus HEXXH(X)18E motif found in the M1 family of zinc-metallopeptidase proteins. In this study, a recombinant soluble form of P-LAP found in maternal serum was expressed in Chinese hamster ovary cells, purified to homogeneity and then characterized. Although N-terminal sequencing revealed a four-amino-acid deletion, the purified enzyme was active and was shown to be a zinc-containing homodimeric protein with molecular mass of 280 kDa in solution. Using artificial substrates, it was shown that the enzyme has broad specificity and is inhibited by several compounds known as aminopeptidase inhibitors. Subsequently, sequential N-terminal amino-acid liberation of several peptide hormones by the enzyme was monitored and structures of the products were determined. Among the hormones having a cysteine residue at their N-terminal end and intramolecular disulfide bonds, it was found that vasopressin and oxytocin, but not calcitonin and endothelins, were cleaved by the enzyme. Because the molecular properties of oxytocinase so far reported often conflict, our results provide an initial biochemical and enzymatic characterization of moleculary defined P-LAP/oxytocinase.
Supplementary Data 1 from SLFN5 Regulates LAT1-Mediated mTOR Activation in Castration-Resistant Prostate Cancer (2023)

&lt;p&gt;Proteomics_CRPC&lt;/p&gt;
Development of an ICP source for the study of helium insertion in tungsten (2013)

International audience
Uniform-size hydrophobic polymer-based separation media selectively modified with a hydrophilic external polymeric layer (1995)

A simple procedure for the preparation of macroporous hydrophobic styrene-divinylbenzene polymeric separation media with a hydrophilic outer surface has been developed. A hydrophilic monomer and water-soluble polymerization initiator are added to the reaction mixture during the final polymerization step of the preparation of size-monodisperse particles. Because the hydrophobic styrene-divinylbenzene framework of the beads is already formed, and the hydrophilic monomer does not penetrate the pores of the beads that are filled with a hydrophobic porogen, the hydrophilic layer is formed only at the surface of the beads. The hydrophilic monomers used included glycerol monomethacrylate and glycerol dimethacrylate and toluene was used as the porogen for the poly(styrene-co-divinylbenzene) beads. Comparative experiments involving beads with or without a hydrophilic medium showed that the separation selectivity of the media towards hydrophobic solutes remains unchanged. However, the modified medium with a hydrophilic layer could be used to analyse mixtures that also contained large peptide molecules as these do not adsorb at its surface as is the case with the unmodified hydrophobic beads.
Recent contributions of electronic circular dichroism to the investigation of oligopeptide conformations (2004)

Abstract Recent applications in our laboratories of electronic circular dichroism to the study of peptide secondary structures and their changes under external stimuli are briefly reviewed. More specifically, this article deals with: 1) characterization of a novel peptide conformation; 2) origin of amino acid homo‐chirality on Earth; 3) bend and helical peptides as spacers; and 4) transfer and propagation of chirality in peptides. Chirality 16:388–397, 2004. © 2004 Wiley‐Liss, Inc.
Si-based Mach-Zehnder wavelength/mode multi/demultiplexer for a WDM/MDM transmission system (2018)

We propose and experimentally demonstrate a low-loss and low-crosstalk Mach-Zehnder mode/wavelength multi/demultiplexer for WDM/MDM transmission based on a Si-photonics platform. A broadband 3-dB mode divider, which is also newly devised here, makes it possible to compose a Mach-Zehnder filter for "mode" and "wavelength" simultaneously. Transmission characteristics of fabricated 3-dB mode dividers are in excellent agreement with theoretical results. Mach-Zehnder filters using the 3-dB mode divider with a free spectral range (FSR) of 20 and 1 nm are also fabricated and the modal crosstalk is less than -24 dB in the 40-nm wavelength range for the MZ filter with an FSR of 20 nm. The tuning of the peak wavelength position by the TiN heater is also demonstrated.
Photoelectron, infrared, and theoretical study of 1-aza-5-boratricyclo[3.3.3.01,5]undecane and related compounds (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhotoelectron, infrared, and theoretical study of 1-aza-5-boratricyclo[3.3.3.01,5]undecane and related compoundsKeui Jen Lee, Peter D. Livant, Michael L. McKee, and S. D. WorleyCite this: J. Am. Chem. Soc. 1985, 107, 21, 5901–5906Publication Date (Print):October 1, 1985Publication History Published online1 May 2002Published inissue 1 October 1985https://pubs.acs.org/doi/10.1021/ja00307a013https://doi.org/10.1021/ja00307a013research-articleACS PublicationsRequest reuse permissionsArticle Views195Altmetric-Citations18LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Estimation of traffic platoon structure from headway statistics (1979)

This paper deals first with the modeling of urban traffic headway statistics. It is shown that a composite distribution based on the convex combination of a lognormal and a shifted exponential distribution gives a good fit to observed traffic data. This statistical model is then used to generate a model for the formation and passage of "platoons" of vehicles. It is shown that the problem of estimating the time at which a "platoon" passes a detector, as well as the number of vehicles in the "platoon," corresponds to the point process disorder problem. An optimal estimator for the platoon size and passage time, based on detector data, is then derived via known results for the point process disorder problem. It is shown that the computations required by this estimator can be performed in a microprocessor. Furthermore, the estimator is tested against the UTCS-1 traffic simulator and performs very well. Parameter sensitivity analysis of the estimator is presented. Finally, the use of these results to improve the filter/predictor described in a companion paper, and vice versa, is explained.
Genetic architecture of human plasma lipidome and its link to cardiovascular disease (2019)

Understanding genetic architecture of plasma lipidome could provide better insights into lipid metabolism and its link to cardiovascular diseases (CVDs). Here, we perform genome-wide association analyses of 141 lipid species (n = 2,181 individuals), followed by phenome-wide scans with 25 CVD related phenotypes (n = 511,700 individuals). We identify 35 lipid-species-associated loci (P &amp;lt;5 ×10-8), 10 of which associate with CVD risk including five new loci-COL5A1, GLTPD2, SPTLC3, MBOAT7 and GALNT16 (false discovery rate&amp;lt;0.05). We identify loci for lipid species that are shown to predict CVD e.g., SPTLC3 for CER(d18:1/24:1). We show that lipoprotein lipase (LPL) may more efficiently hydrolyze medium length triacylglycerides (TAGs) than others. Polyunsaturated lipids have highest heritability and genetic correlations, suggesting considerable genetic regulation at fatty acids levels. We find low genetic correlations between traditional lipids and lipid species. Our results show that lipidomic profiles capture information beyond traditional lipids and identify genetic variants modifying lipid levels and risk of CVD.
Calorie Restriction Promotes Mitochondrial Biogenesis by Inducing the Expression of eNOS (2005)

Calorie restriction extends life span in organisms ranging from yeast to mammals. Here, we report that calorie restriction for either 3 or 12 months induced endothelial nitric oxide synthase (eNOS) expression and 3',5'-cyclic guanosine monophosphate formation in various tissues of male mice. This was accompanied by mitochondrial biogenesis, with increased oxygen consumption and adenosine triphosphate production, and an enhanced expression of sirtuin 1. These effects were strongly attenuated in eNOS null-mutant mice. Thus, nitric oxide plays a fundamental role in the processes induced by calorie restriction and may be involved in the extension of life span in mammals.
Mutation Analysis in Cystic Fibrosis (1990)

The article by Lemna et al. (Feb. 1 issue)1 furthers the evaluation of the ΔF508 mutation, which is associated with some cases of cystic fibrosis. Although its real effect may be to help in documenting the substantial clinical variation that can occur among persons who possess the same small genetic deletion, the finding has encouraged calls for general screening...