gebhart/patrepeval huggingface dataset:
Using miRNA-mRNA Interaction Analysis to Link Biologically Relevant miRNAs to Stem Cell Identity Testing for Next-Generation Culturing Development (2016)

Human adult marrow stromal stem cells have shown great potential in addressing unmet health care needs. Quality assurance is imperative to make advancements in large-scale manufacturing procedures. MicroRNAs are master regulators of biological processes and potentially ideal quality markers. MicroRNA and mRNA profiling data of two human adult stem cell types were correlated to biological functions in silico. Doing this provided evidence that differentially expressed microRNAs are involved in regulating specific stem cell features. Furthermore, expression of a selected microRNA panel was maintained in next-generation culturing platforms, demonstrating the robustness of microRNA profiling in stem cell comparability testing.
Influence of Pokémon Go on Physical Activity: Study and Implications (2016)

Mobile apps combining game play with physical activity lead to substantial short-term activity increases and, in contrast to many existing interventions and mobile health apps, have the potential to reach activity-poor populations. Future studies are needed to investigate potential long-term effects of these applications.
Correlation for the Sauter Mean Diameter of a Prefilmer Airblast Atomizer at Varying Operating Conditions (2016)

Abstract One of the key factors that affect the combustion performance in gas turbines and therefore the amount of pollutant emission and the efficiency is the atomization quality of the liquid fuel. Often reliable droplet size distributions for combustion simulations are missing. In this work a correlation for the Sauter mean diameter based on energy equilibrium is derived and compared to results from three‐dimensional numerical simulation of the atomization of a liquid film and to an empirical correlation from the literature. It is shown that the derived correlation agrees better with the results of the simulations than the empirical correlation.
Reference Governor for Hybrid Dynamical Systems (2022)

We formulate a reference governor algorithm for hybrid systems modeled as hybrid equations, in which the continuous dynamics are governed by a constrained differential equation and the discrete dynamics by a constrained difference equation. Basic definitions, models, and properties of the proposed hybrid reference governor approach are introduced and a time-based implementation is formulated. We apply the methodology to hybrid equations with linear right-hand side in both the differential and difference equations and with explicit logic variables. We illustrate the approach in examples.
Substitution therapy (2020)

Emicizumab is a bispecific antibody that recognizes FIX(a)/FX, and mimics FVIIIa cofactor activity. Due to its unique characteristics including longer half-life and subcutaneous injectability, treatment for haemophilia A dramatically improved regardless of the presence of FVIII inhibitor. Protection from pathological change in joints, avoidance of inhibitor development and intra-cranial haemorrhage could be expected by introduction of emicizumab in early childhood. Applications in mild/moderate patients should be also considered. Clinical assessment tool should be standardized; however, since there are limitations to conventional ABR-based assessment. Laboratory monitoring is another practical issue due to the mode of action of emicizumab. Chromogenic assays and global assays could be utilized. The other emicizumab-related practical issue is immune tolerance induction for the inhibitor patients, since ITI remains the only effective means to inhibitor eradication. With the recently introduced Atlanta protocol, emicizumab prophylaxis is given in combination with 50-100 IU/kg FVIII three times a week. A single manuscript has been published, and multiple clinical trials are open to address the efficacy of this strategy. Whether the Atlanta protocol will be fully embraced is yet to be seen, but there is widespread consensus about attempts to tolerize every haemophilia A patient with an inhibitor.
Polarization investigation of a tunable high-speed short-wavelength bulk-micromachined MEMS-VCSEL (2012)

We report the investigation of the state of polarization (SOP) of a tunable vertical-cavity surface-emitting laser (VCSEL) operating near 850 nm with a mode-hop free single-mode tuning range of about 12 nm and an amplitude modulation bandwidth of about 5 GHz. In addition, the effect of a sub-wavelength grating on the device and its influence on the polarization stability and polarization switching has been investigated. The VCSEL with an integrated sub-wavelength grating shows a stable SOP with a polarization mode suppression ratio (PMSR) more than 35 dB during the tuning.
UV Excitation Thermal Lens Microscope for Sensitive and Nonlabeled Detection of Nonfluorescent Molecules (2006)

An ultrasensitive and nonlabeled detection method of nonfluorescent molecules on a microchip was developed by realizing a thermal lens microscope (TLM) with a 266-nm UV pulsed laser as an excitation light source (UV-TLM). Pulsed laser sources have advantages over continuous-wave laser sources in more compact size and better wavelength tuning, which are important for microchip-based analytical systems. Their disadvantage is difficulty in applying a lock-in amplifier due to the high (>10(4)) duty ratio of pulse oscillation. To overcome this problem, we realized a quasi-continuous-wave excitation by modulating the pulse trains at approximately 1 kHz and detecting the synchronous signal with a lock-in amplifier. The optimum pulse repetition frequency was obtained at 80 kHz, which was reasonable considering thermal equilibrium time. Furthermore, a permissible flow velocity in the range of 6.6-19.8 mm/s was found to avoid sensitivity decrease due to photochemical reactions and thermal energy dissipation. Under these conditions, we detected adenine aqueous solutions on a fused-silica microchip without labeling and obtained a sensitivity that was 350 times higher than that in a spectrophotometric method. The sensitivity was enough for detection on a microchip with an optical path length that was 2-3 orders shorter than that in conventional cuvettes. Finally, the UV-TLM method was applied to liquid chromatography detection. Fluorene and pyrene were separated in a microcolumn and detected in a capillary (50-microm inner diameter) with 150 times higher sensitivity than a spectrophotometric method. Our method provides highly sensitive and widely applicable detections for various analytical procedures and chemical syntheses on microchips.
FlowSculpt: software for efficient design of inertial flow sculpting devices (2019)

Flow sculpting is a powerful method for passive flow control that uses a sequence of bluff-body structures to engineer the structure of inertially flowing microfluidic streams. A variety of cross-sectional flow shapes can be created through this method, offering a new platform for flow manipulation or material fabrication useful in bioengineering, manufacturing, and chemistry applications. However, the inverse problem in flow sculpting - designing a device that produces a target fluid flow shape - remains challenging due to the complex, diverse, and enormous design space. Solutions to the inverse problem have been constrained to single-material fluid streams that are shaped into top-bottom symmetric shapes due to the bluff-body structures available in current libraries (pillars) that span the height of the channel. In this work, we introduce multi-material design and symmetry-breaking flow deformations enabled by half-height pillars, presented within an extremely fast simulation method for flow sculpting yielding a 34-fold reduction in runtime. The framework is deployed freely as a cross-platform application called "FlowSculpt". We detail its implementation and usage, and discuss the addition of enhanced search operations, which enable users to more easily design flow shapes that replicate their input drawings. With FlowSculpt, the microfluidics community can now quickly design flow shaping microfluidic devices on modest hardware, and easily integrate these complex physics into their research toolkit.
ChemInform Abstract: SYNTHESIS OF THE CHIRAL ACYCLONUCLEOSIDE ANTIHERPETIC AGENT (S)‐9‐(2,3‐DIHYDROXY‐1‐PROPOXYMETHYL)GUANINE (1985)

Abstract Die im Titel genannte Verbindung (V), die wegen ihrer potentiellen antiviralen Eigenschaften von Bedeutung ist, wird auf dem angegebenen Weg aus dem Pyranosid (I) erhalten.
THE PALMAR LOCKING COMPRESSION PLATE IS BIOMECHANICALLY COMPARABLE TO THE DORSAL PI PLATE FOR DORSALLY COMMINUTED, INTRAARTICULAR WRIST FRACTURES (2007)

A clinically appropriate fracture model and testing regimen were used to test the null hypothesis that a palmarly applied locking plate was inferior to a dorsally applied Pi plate in the stabilisation of dorsally comminuted intraarticular wrist fractures. Sixteen standardised fractures of Synbone models of the radius were stabilised using either a palmar locking compression T plate (the experimental group) (n=8) or a dorsally applied Pi plate (the control group) (n=8). The constructs were tested on an Instron materials testing machine. Deformation was monitored during 500 loading cycles to 200 N. The mean permanent deformation and stiffness favoured the palmar locking compression T plate over the dorsal Pi plate (P=0.036). However, the absolute difference was only 0.5 mm. Such a small difference is unlikely to be clinically detectable and, therefore, we conclude that there is no clinically significant difference between the two types of fixation.
Effects of Age and Gender on Autonomic Control of Blood Pressure Dynamics (1999)

Abstract —Both age and gender influence cardiovascular autonomic control, which in turn may influence the ability to withstand adverse cardiac events and respond to orthostatic stress. The purpose of this study was (1) to quantify age- and gender- related alterations in autonomic control of blood pressure (BP) and (2) to examine the impact of these autonomic alterations on BP response to orthostatic stress. We measured continuous BP and R-R intervals and vasoactive peptide levels in the supine and 60° head-up tilt positions during paced respiration (0.25 Hz) in 89 carefully screened healthy subjects (41 men, 48 women, aged 20 to 83 years). Data were analyzed by gender (age adjusted) and by age group (gender adjusted). During tilt, women had greater decreases in systolic BP than men (−10.2±2 versus −1.2±3 mm Hg; P =0.02) and smaller increases in low-frequency (sympathetically mediated) BP power ( P =0.02). Upright plasma norepinephrine was lower in women ( P =0.02). Women had greater supine high-frequency R-R interval power than men ( P =0.0001). In elderly subjects, the tilt-induced increase in low-frequency BP power was also diminished ( P =0.01), despite higher supine ( P =0.02) and similar upright norepinephrine levels compared with younger subjects. Thus, healthy women have less sympathetic influence on BP and greater parasympathetic influence on R-R interval than men. Elderly subjects also have reduced sympathetic influence on BP, but this appears to be more consistent with a reduction in vasomotor sympathetic responsiveness.
Correction to Convergent Kilo-Scale Synthesis of a Potent Renin Inhibitor for the Treatment of Hypertension (2012)

ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionNEXTORIGINAL ARTICLEThis notice is a correctionCorrection to Convergent Kilo-Scale Synthesis of a Potent Renin Inhibitor for the Treatment of HypertensionLouis-Charles Campeau*, Sarah J. Dolman, Danny Gauvreau, Ed Corley, Jinchu Liu, Erin N. Guidry, Stéphane G. Ouellet, Dietrich Steinhuebel, Mark Weisel, and Paul D. O’SheaCite this: Org. Process Res. Dev. 2012, 16, 6, 1241Publication Date (Web):May 29, 2012Publication History Published online29 May 2012Published inissue 15 June 2012https://doi.org/10.1021/op300123uCopyright © 2012 American Chemical SocietyRIGHTS & PERMISSIONSArticle Views1103Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (114 KB) Get e-Alertsclose Get e-Alerts
Multiuser detection with an unknown number of users (2005)

This correspondence studies the problem of separating a signal of interest from co-channel interference for the situation when the number of co-channel users and their channels are unknown. We devise a novel approach to this problem based on a trained statistical mixture model. Numerical results illustrate that the new method can outperform conventional training-based multiuser detection.
Effects of Retinylacetate on the Kinetics of Rat Liver Guanylate Cyclase: Possible Interaction with Sulfhydryl Groups (1985)

The effect of retinylacetate (RA) was investigated on rat liver guanylate cyclase stimulated by nitroprusside (NP). The stimulated enzyme seemed to adhere to Michaëlis-Menten kinetics with an apparent Km for MnGTP of 0.10 mM and a Vmax of 410 pmol cGMP/min. X mg prot. RA (0.1-1mM) dose-dependently inhibited the enzyme stimulated by NP (0.1-10 mM). In the presence of 1 mM RA the activity was only 10% of the control activity. The inhibitory action of RA seemed to be non-competitive since it depressed Vmax without affecting the Km for MnGTP. In contrast, however, RA was instead found to stimulate the enzyme when low concentrations of NP (0.01-0.1 mM) were used. The concentration-activity curve for NP was bell-shaped showing an optimum at 0.5 mM. The inhibition induced by RA could not be surmounted by increasing the NP concentration indicating that RA did not compete with NP. A bell-shaped activity curve was also seen when the enzyme activity was measured in the presence of increasing Mn2+ concentrations and during these conditions RA also caused inhibition. In the presence of the sulphydryl reductant dithiothreitol (DTT), the NP concentration needed for optimal enzyme activation was about 100-fold less than in the absence of DTT. The maximal enzyme activity was also slightly increased. In the presence of DTT, RA was much less effective to induce inhibition of the stimulated enzyme, than when DDT was absent. (In the presence of DTT 1 mM RA caused only 30% inhibition compared to 90% inhibition in its absence).(ABSTRACT TRUNCATED AT 250 WORDS)
CX3CR1 deficiency induces an early protective inflammatory environment in ischemic mice (2013)

The studies on fractalkine and its unique receptor CX3CR1 in neurological disorders yielded contrasting results. We have explored the consequences of CX3CR1 deletion in ischemic (30' MCAo) mice on: (1) brain infarct size; (2) microglia dynamism and morphology; (3) expression of markers of microglia/macrophages (M/M) activation and polarization. We observed smaller infarcts in cx3cr1(-/-) (26.42 ± 7.41 mm(3) , mean ± sd) compared to wild type (36.29 ± 11.57) and cx3cr1(-/+) (34.49 ± 8.91) mice. We longitudinally analyzed microglia by in vivo two-photon microscopy before, 1 and 24 h after transient ischemia. Microglia were stationary in both cx3cr1(-/-) and cx3cr1(-/+) mice throughout the study. In cx3cr1(-/-) mice, they displayed a significantly higher number of ramifications >10 μm at baseline and at 24 h after ischemia compared to cx3cr1(-/+) mice, indicating that CX3CR1 deficiency impaired the development of microglia hypertrophic/amoeboid morphology. At 24 h after ischemia, we performed post mortem quantitative immunohistochemistry for different M/M markers. In cx3cr1(-/-) immunoreactivity for CD11b (M/M activation) and for CD68 (associated with phagocytosis) were decreased, while that for CD45(high) (macrophage and leukocyte recruitment) was increased. In addition, immunoreactivity for Ym1 (M2 polarization) was enhanced, while that for iNOS (M1) was decreased. Our data show that in cx3cr1(-/-) mice protection from ischemia at early time points after injury is associated with a protective inflammatory milieu, characterized by the promotion of M2 polarization markers.
Processing Optimization of Ecklonia cava Extract-Added Seasoning Sauce for Instant Noodles (2011)

This study was conducted to optimize processing of functional seasoning sauce for instant noodles (SSIN) using response surface methodology (RSM), and to compare the functional properties of commercial SSIN. Central composite designs were adopted in the SSIN processing for ingredient formula optimization. Concentrations of sea tangle (<TEX>$X_1$</TEX>), traditional soy sauce (<TEX>$X_2$</TEX>), yeast extract (<TEX>$X_3$</TEX>) and Ecklonia cava extract (ECE) (<TEX>$X_4$</TEX>) were chosen as independent variables. The dependent variables were glutamic acid content (<TEX>$Y_1$</TEX>), score of sensory evaluation (<TEX>$Y_2$</TEX>), and antioxidative activity (<TEX>$Y_3$</TEX>). We found the optimal conditions to be <TEX>$X_1$</TEX>=3.91%, <TEX>$X_2$</TEX>=20.57%, <TEX>$X_3$</TEX>=3.04% and <TEX>$X_4$</TEX>=3.78%. The predicted values of the multiple response optimal conditions were <TEX>$Y_1$</TEX>=124.0 mg/100 g, <TEX>$Y_2$</TEX>=7.6 and <TEX>$Y_3$</TEX>=1.95. The antioxidative activity (PF, PF=oil induction period with sauce/oil induction period with distilled water) and ACE inhibitory activity of ECE-added SSIN were 1.98 and 29.0%, respectively, which were significantly higher than those of commercial SSIN (1.09 and 4.4%, respectively).
ME-143, a novel inhibitor of tumor-specific NADH oxidase (tNOX): Results from a first-in-human phase I study. (2012)

3067 Background: ME-143, a second generation isoflavone-derived compound, specifically binds tNOX, shifting the ceramide-S1P equilibrium and resulting in prompt apoptosis via pleotrophic caspase activation. A first generation compound, phenoxodiol (PXD), showed promising phase II activity with cisplatin in ovarian cancer. ME-143 is broadly active against human cancers in both in vitro and in vivo models, with IC 50 ’s 1-2 logs lower than PXD. Toxicology studies up to 140mg/kg (human dose equivalent ~23mg/kg) showed no STD level. The only significant findings were dose dependent hypospermia and testicular atrophy in rats. We report clinical and PK results from the first-in-human phase I study. Methods: A 3+3 dose escalation design was used with 4 dose cohorts: 2.5, 5, 10, and 20mg/kg IV over 30 minutes weekly times 3, followed by a 1 week break, and then continuous weekly dosing in patients with advanced solid tumors. Dense PK sampling was performed at 0, 5, 10, 20, 30, 60, 90, 120, 180, 240, 300, 360 minutes, and 24 hours post-infusion day 1 and 15 of the first treatment cycle. Results: To date, 9 patients have been enrolled, 3 in each of the first 3 cohorts. Median time on treatment is 56 days (range 6 to 62). Five patients have discontinued protocol therapy, all due to PD. No DLT’s have been observed. Related AE’s include grade 1: myalgia (1), anorexia (1), fatigue (2), headache (1), diarrhea (1), vomiting (1) and grade 2: fatigue (1). Preliminary PK analyses in the first 2 cohorts is shown in table below. Conclusions: ME-143 appears to be well tolerated when administered IV. Preliminary PK data suggest that drug levels achieve target concentration extrapolated from pre-clinical studies (AUC 0-t ~10mg*hr/mL) and exceed levels obtained with IV PXD in the phase II study (AUC 0-t ~2mg*hr/mL) . Updated clinical data from all planned dose cohorts, including the final pharmacokinetic analysis and planned phase II dose, will be presented. [Table: see text]
Sézary syndrome and mycosis fungoides: An overview, including the role of immunophenotyping (2020)

This review discusses the definition and major categories of cutaneous T-cell lymphoma, Sézary syndrome and mycosis fungoides, and the role of immunophenotyping in their diagnosis. The following key points are raised: (a) Sézary syndrome and mycosis fungoides cells most often have a characteristic CD3+ CD4+ CD7- and/or CD26- immunophenotype. (b) This immunophenotype is not specific, but can assist in the distinction from non-neoplastic T cells and other subtypes of mature T-cell neoplasm. (c) However, small subsets of normal and reactive T-cells can have an overlapping immunophenotype, and can be distinguished by evaluating for additional changes in antigen expression.
Application of the Jet CutterTM technology for the production of spherical beads from highly viscous polymer solutions (1999)

The jet cutting method allows the production of spherical beads in the range of 0.2 to 3 mm in diameter even from highly viscous fluids (e.g. polyvinyl alcohol or alginate solutions) with high throughput and narrow particle size distribution. This technique may be useful for applications in biotechnology and in the pharmaceutical, chemical or food industry
COMPARISON BETWEEN MONOPOLAR AND TRIPOLAR CONFIGURATIONS IN (1997)

Nerve cuff electrodes have been shown safe and able to activate selectively specific fascicles in a nerve trunk. Selectivity has been shown using a cuff with four radially placed tripoles thus requiring twelve contacts and twelve lead wires. This study evaluates a simplified cuff electrode, consisting of four radially placed monopole electrodes, requiring only four lead wires, and compares the results to the tripolar electrode configuration. Experiments were performed on two cats, each with an electrode that was implanted for over six months. Results from a correlation analysis and a torque vector likeness measure indicated that the recruitment characteristics of the two configurations were similar in 6 of 8 cases.
Galactosyl cyclitol accumulation enhanced by substrate feeding of soybean embryos. (2003)

A study was conducted to determine if feeding free cyclitols to soyabean embryos enhances the accumulation of galactosyl cyclitols, and if feeding D-chiro-inositol and D-pinitol combined with soyabean embryos reduces fagopyritol B1 and galactopinitol accumulation below the amounts observed after feeding D-chiro-inositol and D-pinitol individually. Treatments comprised: 0, 10, 25, 50 and 100 mM myo-inositol, D-chiro-inositol or D-pinitol combined with 100, 90, 75, 50 and 0 mM sucrose. Embryos were incubated in either 30 mM myo-inositol + 100 mM sucrose, 100 mM D-chiro-inositol or 100 mM D-pinitol for 24 h, blotted and slow dried for 0, 1, 2, 3, 4 or 14 days. Myo-inositol up to 50 mM increased free myo-inositol slightly (D-pinitol and D-chiro-inositol even less). Elevated myo-inositol concentrations decreased as galactinol increased transiently during days 2 and 3 and then galactinol decreased as raffinose and stachyose accumulated. After feeding sucrose with myo-inositol, sucrose concentration decreased, and raffinose and stachyose concentrations increased during slow drying. D-chiro-inositol increased free D-chiro-inositol by 50-fold and fagopyritol B1 by 7-fold. A large fagopyritol B1 increase between days 2 and 4 during slow drying was accompanied by free D-chiro-inositol decrease. A transient galactinol increase preceded the increase in raffinose and stachyose. Feeding D-pinitol increased D-pinitol, galactopinitol A and galactopinitol B concentrations by 8.0-, 4.5- and 4.2-fold, respectively. Myo-inositol decreased as galactinol increased during days 2 and 3, and galactinol decreased as raffinose and stachyose accumulated. Feeding sucrose at 0 to 200 mM resulted in little change in concentrations of soluble carbohydrates.