gebhart/patrepeval huggingface dataset:
Lysis of malignant B cells from patients with B-chronic lymphocytic leukemia by autologous T cells activated with CD3 x CD19 bispecific antibodies in combination with bivalent CD28 antibodies (1993)

Abstract Bispecific antibodies (bi-MABs) can be used to target T cells to autologous tumor cells. It has been shown that the activation of resting human T cells requires two independent signals, namely the cross-linking of the T-cell receptor (TCR)-CD3 complex together with the CD28 homodimer. In the present study, we demonstrate the activation of T cells from patients with chronic lymphocytic leukemia (CLL) using bi-MABs against the CD3 and CD19 antigens (CD3 x CD19) in combination with monospecific, bivalent antibodies against the CD28 antigen. Mononuclear cells from patients with CLL were cultured with the bi-MAB CD3 x CD19 and monospecific CD28 antibodies. The CD3 x CD19 bi-MABs were isolated by the hybridoma-hybridoma fusion technique and purified by hydrophobic interaction chromatography. T-Cell activation as demonstrated by increased proliferation, upregulation of T-cell activation markers (CD25, CD38), and cytotoxicity against autologous CLL cells and allogeneic B cells was shown in seven of eight CLL specimens. The stimulation with CD3 x CD19 bi-MABs with CD28 antibodies preferentially induced proliferation of CD4+ T cells. The effective dose of purified antibodies required for optimal T-cell activation was 100 ng/mL in vitro, which suggests that this antibody combination may be useful for immunotherapy of patients with B-CLL.
CCM3/PDCD10 Heterodimerizes with Germinal Center Kinase III (GCKIII) Proteins Using a Mechanism Analogous to CCM3 Homodimerization (2011)

CCM3 mutations give rise to cerebral cavernous malformations (CCMs) of the vasculature through a mechanism that remains unclear. Interaction of CCM3 with the germinal center kinase III (GCKIII) subfamily of Sterile 20 protein kinases, MST4, STK24, and STK25, has been implicated in cardiovascular development in the zebrafish, raising the possibility that dysregulated GCKIII function may contribute to the etiology of CCM disease. Here, we show that the amino-terminal region of CCM3 is necessary and sufficient to bind directly to the C-terminal tail region of GCKIII proteins. This same region of CCM3 was shown previously to mediate homodimerization through the formation of an interdigitated α-helical domain. Sequence conservation and binding studies suggest that CCM3 may preferentially heterodimerize with GCKIII proteins through a manner structurally analogous to that employed for CCM3 homodimerization.
Optical Signature of Delocalized Polarons in Conjugated Polymers (2001)

Quantum-chemical calculations, incorporating both electron–phonon and electron–electron effects, are performed on two-chain model aggregates to assess the influence of interchain interactions on the nature of the singly charged species (polarons) in organic conjugated polymers. In symmetric cofacial dimers with fixed intermolecular distances, our theoretical approach leads to polarons fully delocalized over the two conjugated chains. Such a delocalization strongly affects the geometric and electronic relaxation phenomena induced by charge injection, which in turn leads to a dramatic spectral redistribution of the linear absorption cross section. Optical signatures for delocalized polarons in conjugated materials are identified from our theoretical description and compared to experimental data from optical charge modulation spectroscopy; these are fingerprints for the degree of interchain order, a decisive factor in the achievement of high charge carrier mobilities in polymer-based field-effect transistors.
3-Cephen chromophore (1971)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT3-Cephen chromophoreRamanathan Nagarajan and Douglas O. SpryCite this: J. Am. Chem. Soc. 1971, 93, 9, 2310–2312Publication Date (Print):May 1, 1971Publication History Published online1 May 2002Published inissue 1 May 1971https://pubs.acs.org/doi/10.1021/ja00738a036https://doi.org/10.1021/ja00738a036research-articleACS PublicationsRequest reuse permissionsArticle Views52Altmetric-Citations18LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Synthesis of 4-phenyl-5H-pyrido[3,4-b][1,4]benzothiazin-3(2H)-ones (4-phenylazaphenothiazines) via activated dimethylformamide reagents (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of 4-phenyl-5H-pyrido[3,4-b][1,4]benzothiazin-3(2H)-ones (4-phenylazaphenothiazines) via activated dimethylformamide reagentsRobert J. Chorvat, Suzanne Evans Radak, and Bipin N. DesaiCite this: J. Org. Chem. 1987, 52, 7, 1366–1369Publication Date (Print):April 1, 1987Publication History Published online1 May 2002Published inissue 1 April 1987https://pubs.acs.org/doi/10.1021/jo00383a044https://doi.org/10.1021/jo00383a044research-articleACS PublicationsRequest reuse permissionsArticle Views86Altmetric-Citations6LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
TIM-family proteins inhibit HIV-1 release (2014)

Accumulating evidence indicates that T-cell immunoglobulin (Ig) and mucin domain (TIM) proteins play critical roles in viral infections. Herein, we report that the TIM-family proteins strongly inhibit HIV-1 release, resulting in diminished viral production and replication. Expression of TIM-1 causes HIV-1 Gag and mature viral particles to accumulate on the plasma membrane. Mutation of the phosphatidylserine (PS) binding sites of TIM-1 abolishes its ability to block HIV-1 release. TIM-1, but to a much lesser extent PS-binding deficient mutants, induces PS flipping onto the cell surface; TIM-1 is also found to be incorporated into HIV-1 virions. Importantly, TIM-1 inhibits HIV-1 replication in CD4-positive Jurkat cells, despite its capability of up-regulating CD4 and promoting HIV-1 entry. In addition to TIM-1, TIM-3 and TIM-4 also block the release of HIV-1, as well as that of murine leukemia virus (MLV) and Ebola virus (EBOV); knockdown of TIM-3 in differentiated monocyte-derived macrophages (MDMs) enhances HIV-1 production. The inhibitory effects of TIM-family proteins on virus release are extended to other PS receptors, such as Axl and RAGE. Overall, our study uncovers a novel ability of TIM-family proteins to block the release of HIV-1 and other viruses by interaction with virion- and cell-associated PS. Our work provides new insights into a virus-cell interaction that is mediated by TIMs and PS receptors.
Functional Kinomics Identifies Candidate Therapeutic Targets in Head and Neck Cancer (2014)

WEE1 kinase is a potential therapeutic drug target for HNSCC. This study supports the application of a functional kinomics strategy to identify novel therapeutic targets for cancer.
Effects of Geniposide from Gardenia Fruit Pomace on Skeletal-Muscle Fibrosis (2018)

Geniposide is the main bioactive constituent of gardenia fruit. Skeletal-muscle fibrosis is a common and irreversibly damaging process. Numerous studies have shown that geniposide could improve many chronic diseases, including metabolic syndrome and tumors. However, the effects of geniposide on skeletal-muscle fibrosis are still poorly understood. Here, we found that crude extracts of gardenia fruit pomace could significantly decrease the expression of profibrotic genes in vitro. Moreover, geniposide could also reverse profibrotic-gene expression induced by TGF-β and Smad4, a regulator of skeletal-muscle fibrosis. In addition, geniposide treatment could significantly downregulate profibrotic-gene expression and improve skeletal-muscle injuries in a mouse model of contusion. These results together suggest that geniposide has an antifibrotic effect on skeletal muscle through the suppression of the TGF-β-Smad4 signaling pathway.
2008 Best of the Rest in Urological Survey (2008)

No AccessJournal of Urology2008 Best of the Rest in Urological Survey1 Dec 20082008 Best of the Rest in Urological Survey Fray F. Marshall, M.D. James E. Montie, M.D. Patrick C. Walsh, M.D. Ralph V. Clayman, M.D. David A. Goldfarb, M.D. Craig Niederberger, M.D. Allen D. Seftel, andM.D. Douglas A. CanningM.D. Fray F. MarshallFray F. Marshall More articles by this author , James E. MontieJames E. Montie More articles by this author , Patrick C. WalshPatrick C. Walsh More articles by this author , Ralph V. ClaymanRalph V. Clayman More articles by this author , David A. GoldfarbDavid A. Goldfarb More articles by this author , Craig NiederbergerCraig Niederberger More articles by this author , Allen D. SeftelAllen D. Seftel More articles by this author , and Douglas A. CanningDouglas A. Canning More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2008.09.065AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Hospital Lymph Node Counts and Survival After Radical Cystectomy 1 : Extent of pelvic lymphadenectomy and its impact on outcome in patients diagnosed with bladder cancer: analysis of data from the Surveillance, Epidemiology and End Results Program data base. J Urol2003; 169: 946. Link, Google Scholar Five-Alpha-Reductase Inhibitors for Prostate Cancer Prevention 1 : New take on a prostate drug and a new debate. New York Times, Sunday, June 15, 2008; pg 1, 23 http://query.nytimes.com/gst/fullpage.html?res=9E01E1DE123FF936A25755C0A96E9C8B63&scp=2&sq=prostate%20drug%20kolata&st=cse. Google Scholar 2 : Prediction of prostate cancer for patients receiving finasteride: results from the Prostate Cancer Prevention Trial. J Clin Oncol2007; 25: 3076. Google Scholar Erectile Dysfunction as a Predictor of Cardiovascular Events and Death in Diabetic Patients With Angiographically Proven Asymptomatic Coronary Artery Disease: A Potential Protective Role for Statins and 5-Phosphodiesterase Inhibitors 1 : Erectile dysfunction. J Urol, suppl2006; 175: S25. Link, Google Scholar 2 : Erectile dysfunction as a predictor of cardiovascular disease. Int J Impot Res2008; 20: 460. Google Scholar 3 : Erectile dysfunction and subsequent cardiovascular disease. JAMA2005; 294: 2996. Google Scholar © 2008 by American Urological AssociationFiguresReferencesRelatedDetails Volume 180Issue 6December 2008Page: 2286-2296 Advertisement Copyright & Permissions© 2008 by American Urological AssociationMetricsAuthor Information Fray F. Marshall More articles by this author James E. Montie More articles by this author Patrick C. Walsh More articles by this author Ralph V. Clayman More articles by this author David A. Goldfarb More articles by this author Craig Niederberger More articles by this author Allen D. Seftel More articles by this author Douglas A. Canning More articles by this author Expand All Advertisement PDF downloadLoading ...
Control of VSC‐HVDC connected wind farms for system frequency support (2020)

With the increasing offshore wind farms connected into large power grid by VSC-HVDC, the system equivalent inertia has been further reduced, which has brought new challenges to the safe operation of the system. In this article, we proposed a novel strategy to coordinate the rotational kinetic energy of variable speed wind turbines and the capacitor energy of VSC-HVDC to support the main power system frequency. Selection of controller parameters is discussed to improve the frequency control effect. This strategy reduces the rate of change of frequency and improves the frequency nadir, which also limit the wind turbine rotor speed variation range to maintain its stable operation. The proposed strategy can also reduce the secondary frequency drop to a large extent. The proposed strategy is verified by using a power system model in MATLAB/Simulink. The simulation results show that the proposed method can effectively utilize the frequency control capability of VSC-HVDC and the wind farm to maintain frequency stability of the power system.
High-performance PIN photodetector at 67 GHz and beyond for radio over fiber applications (2015)

High-performance photodetectors (PDs) for radio over fiber (RoF) applications over 60 GHz were designed and fabricated. The RF output was investigated while a high linearity was observed for two designs: a low carrier concentration InGaAs absorption layer in a PIN structure and a low carrier concentration collection layer in a unitravelling- carrier (UTC) structure. The RF output performances of both PIN and UTC structures were studied at 67 GHz and 100 GHz respectively. High photocurrent densities could be obtained from both structures (21.7 kA/cm<sup>2</sup> in the PIN structure and 35.4 kA/cm<sup>2 </sup>in the UTC structure). The PIN structure exhibited a slightly higher current density of 1.6 times than the UTC structure. The frequency response of the UTC-PD exhibited excellent flatness up to 110 GHz, with a 3 dB bandwidth beyond 110 GHz. In addition, maximum RF output powers of +6.8 dBm at 67 GHz and -5 dBm at 100 GHz was successfully obtained. The space charge effect could be ruled out for the output linearity, but avoiding overheating in the p-contact metal had to be considered. By modifying impedance matching circuit designs, the maximum RF output power level of 3 dB can be improved.
A DNA polymorphism in close physical linkage with the proopiomelanocortin gene. (1983)

Cellular DNAs from a panel of 20 unrelated individuals were screened for restriction fragment length polymorphisms (RFLP) with a DNA probe containing the first exon of the proopiomelanocortin gene (POMC), which has been assigned to chromosome 2p23-25. Digestion with the restriction endonuclease Sst 1 revealed a high frequency RFLP. The two alleles that were found are fragments of 10- and 15-kilobase (kb) length and are in Hardy-Weinberg equilibrium with frequencies of 72.6% and 27.4%, respectively. Informative families were tested for linkage between POMC/Sst 1 RFLP and other polymorphic markers of chromosome 2. Linkage was excluded to AcP-1 (2p23-25) at 15% recombination, which is still consistent with the chromosomal assignments for these genes. The close physical linkage (10 kb) of the polymorphic locus to the POMC gene makes this RFLP a suitable marker for future linkage studies involving the POMC gene.
Broadly Neutralizing Antibodies to HIV and Their Role in Vaccine Design (2016)

HIV employs multiple means to evade the humoral immune response, particularly the elicitation of and recognition by broadly neutralizing antibodies (bnAbs). Such antibodies can act antivirally against a wide spectrum of viruses by targeting relatively conserved regions on the surface HIV envelope trimer spike. Elicitation of and recognition by bnAbs are hindered by the arrangement of spikes on virions and the relatively difficult access to bnAb epitopes on spikes, including the proximity of variable regions and a high density of glycans. Yet, in a small proportion of HIV-infected individuals, potent bnAb responses do develop, and isolation of the corresponding monoclonal antibodies has been facilitated by identification of favorable donors with potent bnAb sera and by development of improved methods for human antibody generation. Molecular studies of recombinant Env trimers, alone and in interaction with bnAbs, are providing new insights that are fueling the development and testing of promising immunogens aimed at the elicitation of bnAbs.
Enhanced Self-frequency Shift of Cavity Soliton in Mode-locked Octave-spanning Frequency Comb Generation (2014)

We show a significant self-frequency shift of ultrafast cavity solitons associated with Kerr frequency combs in a dispersion-flattened nonlinear cavity. Dispersion induces a frequency shift up to 20% of the pump frequency.
Microbial community function and biomarker discovery in the human microbiome (2011)

Deep exome resequencing is a powerful approach for delineating patterns of protein-coding variation among genes, pathways, individuals and populations. We analyzed exome data from 2,440 individuals of European and African ancestry as part of the National Heart, Lung, and Blood Institute's Exome Project, the aim of which is to discover novel genes and mechanisms that contribute to heart, lung and blood disorders. Each exome was sequenced to a mean coverage of 116, allowing detailed inferences about the population genomic patterns of both common variation and rare coding variation. We identifi ed more than 500,000 single nucleotide variations, the majority of which were novel and rare (76% of variants had a minor allele frequency of less than 0.1%), refl ecting the recent dramatic increase in the size of the human population. The unprecedented magnitude of this dataset allowed us to rigorously characterize the large variation in nucleotide diversity among genes (ranging from 0 to 1.32%), as well as the role of positive and purifying selection in shaping patterns of proteincoding variation and the diff erential signatures of population structure from rare and common variation. This dataset provides a framework for personal genomics and is an important resource that will allow inferences of broad importance to human evolution and health.
Unidirectional HVdc Topology With DC Fault Ride-Through Capability (2017)

HVdc transmission using modular multilevel converters (MMCs) is becoming increasingly prevalent. However, line-commutated converter (LCC) HVdc transmission remains advantageous in its ability to clear dc faults and restart power transmission without operation of the ac system protection. This paper presents an HVdc system topology that employs both MMC and LCC converters to transmit unidirectional power into weak ac systems while retaining the ability to clear dc faults. Control strategies and performance under dc and ac faults are discussed and demonstrated using electromagnetic transient simulations.
Anaphylaxis to packaged foods in Australasia (2018)

Anaphylaxis to undeclared allergens was not rare and did not appear to depend on whether the product was labelled with precautionary advice. There is currently no reliable labelling system that can inform food-allergic consumers of safer food choices. Improvements in the regulation of food labelling with PAL are required.
Delta opioid receptor selective ligands; DPLPE‐deltorphin chimeric peptide analogues† (1994)

Further efforts to correlate the topography of the bioactive structures of DPDPE and the deltorphins, two delta-opioid receptor active peptide families, are reported. A number of DPLPE-deltorphin chimeric peptides have been synthesized in which the C-terminal dipeptide delta-address of the deltorphins (-Val-GlyNH2, -Nle-GlyNH2) have been linked to the highly delta-opioid selective cyclic peptides DPDPE or DPLPE. These studies demonstrate that a major structural feature determining high potency of hybrid analogues is the chirality of the amino acid residue in position 5. The radioligand binding assays have revealed a decrease in potency (compared to DPDPE) at delta-receptors when the C-terminal dipeptides were added to DPDPE. On the other hand, chimeric peptides of DPLPE with these same C-terminal dipeptides retained high delta-selectivity and affinity. Similar results were obtained using the mouse vas deferens (MVD) and guinea pig ileum (GPI) bioassays. The importance of the hydrophilicity of amino acids in positions 2 and 5 for delta-selectivity is consistent with the previous finding for DPLPE and DPDPE. On the other hand, the replacement of phenylalanine-4 with p-chlorophenylalanine-4 did not increase delta-selectivity as in DPDPE. These findings suggest that the delta-receptor interacts with hybridized enkephalins and deltorphins somewhat differently than with DPDPE.
Conveying language through haptics (2018)

In our daily lives, we rely heavily on our visual and auditory channels to receive information from others. In the case of impairment, or when large amounts of information are already transmitted visually or aurally, alternative methods of communication are needed. A haptic language offers the potential to provide information to a user when visual and auditory channels are unavailable. Previously created haptic languages include deconstructing acoustic signals into features and displaying them through a haptic device, and haptic adaptations of Braille or Morse code; however, these approaches are unintuitive, slow at presenting language, or require a large surface area. We propose using a multi-sensory haptic device called MISSIVE, which can be worn on the upper arm and is capable of producing brief cues, sufficient in quantity to encode the full English phoneme set. We evaluated our approach by teaching subjects a subset of 23 phonemes, and demonstrated an 86% accuracy in a 50 word identification task after 100 minutes of training.
Bone Morphogenetic Protein–Inducer Tilorone Identified by High-Throughput Screening Is Antifibrotic In Vivo (2012)

Idiopathic pulmonary fibrosis (IPF) is a progressive lung disease with a poor prognosis and very few therapeutic options. On the molecular level, patients with IPF have increased amounts of the bone morphogenetic protein (BMP) inhibitor gremlin in their lungs, which results in decreased BMP signaling, and an increase in transforming growth factor-β signaling. Based on these findings, we hypothesized that restoration of the impaired BMP signaling would offer a novel strategy for the prevention of fibrosis progression or for the treatment of pulmonary fibrosis. We used reporter cell lines and high-throughput screening of a chemical compound library as an approach to finding molecules that increase BMP signaling in lung epithelial cells, without increasing transforming growth factor-β signaling. The most promising candidate drug was analyzed further by studying its effects on BMP target gene expression, Smad protein phosphorylation, and a mouse model of silica-induced pulmonary fibrosis. The most promising drug candidate, tilorone, induced BMP signaling in the reporter cells and increased the expression of BMP-7 and a BMP target gene, Id3, in lung epithelial A549 cells. In a mouse model of pulmonary fibrosis, tilorone decreased lung hydroxyproline content and the expression of collagen genes Col1A1 and Col3A1. Mice treated with tilorone showed markedly decreased histological changes, compared with untreated mice. These findings indicate that tilorone has biologically significant antifibrotic properties.