gebhart/patrepeval huggingface dataset:
Cytochrome P450 Monooxygenase Activity in the Dark Southern Subterranean Termite (Isoptera: Rhinotermitidae) (1998)

Microsomal oxidases were characterized using surrogate substrates in the economically important dark southern subterranean termite, Reticulitemes virginicus (Banks). Aldrin epoxidase activity required NADPH and was inhibited by carbon monoxide and piperonyl butoxide (I50 = 4.72 ±0.31 × 10-6M), indicating that the enzyme(s) involved was a cytochrome P450 monooxygenase. Aldrin epoxidase activity was highest at 22°C and pH 7.2. Also, the activity was linear up to 0.5 m!(of protein per incubate and increased with reaction time up to 15 min. Although neither substrate nor cofactor was found to be a limiting factor, aldrin epoxidase activity failed to produce a linear response with respect to time at temperatures > 22°C,indicating enzyme inhibition. Although increased incubation temperature >22°C resulted in decreased aldrin epoxidase activity, similar heat treatments did not result in a concomitant increase in cytochrome P420. Significant variation (2.7-fold) in aldrin epoxidase activity was observed among 4 R. virginicus colonies collected from different locations in Florida. Additionally, cytochrome P450 and b5 content, cytochrome c reductase, and methoxyresorufin O-demethylase activities were measured in R. virginicus.
PCR Amplification from Fixed Tissue Indicates Frequent Involvement of Brachyspira aalborgi in Human Intestinal Spirochetosis (1999)

PCR procedures amplifying portions of the 16S rRNA and NADH oxidase genes of Brachyspira aalborgi and Serpulina pilosicoli were applied to DNA extracted from paraffin-embedded human colonic or rectal tissues from 30 Norwegian, Australian, and U.S. patients, 16 of whom had histologic evidence of intestinal spirochetosis (IS). B. aalborgi-specific sequences were identified by PCR in 10 of the IS patients (62.5%) but none of the others, while S. pilosicoli sequences were not detected in tissues from any patient. Direct sequencing of products from three of the positive samples provided further confirmation of the presence of B. aalborgi. B. aalborgi may be a more common cause of intestinal spirochetosis than has been previously thought.
A new view of integrin αIIbβ3 bound to membrane (2013)

In this issue of Blood, Choi et al report a 3-dimensional (3D) reconstruction model of integrin aIIbb3 in its latent state which challenges the existing paradigm and provides new insights into the mechanism of integrin activation.
Purification of Soluble Recombinant Human Tau Protein from Bacteria Using Double-tag Affinity Purification (2018)

Dysfunction of the microtubule-associated protein Tau (encoded by the <i>MAPT</i> gene) has been implicated in more than twenty neurodegenerative diseases, including Alzheimer's. As such, the physiological and disease-relevant functions of Tau have garnered great interest in the research community. One barrier hampering investigations into the functions of Tau and the generation of pharmacological agents targeting Tau has been the difficulty of obtaining soluble Tau protein in purified form. Here, we describe a protocol that uses dual affinity tag purification to selectively purify soluble recombinant Tau protein from bacteria that is functionally active for downstream applications including immunization, microtubule binding assays, and protein-protein interaction studies.
High-efficiency W-band hybrid integrated photoreceiver module using UTC-PD and pHEMT amplifier (2016)

A 100-GHz narrowband photoreceiver module integrated with a zero-bias operational uni-traveling-carrier photodiode (UTC-PD) and a GaAs-based pseudomorphic high-electron-mobility transistor (pHEMT) amplifier was fabricated and characterized. Both devices exhibited flat frequency response and outstanding overall performance. The UTC-PD showed a 3-dB bandwidth beyond 110 GHz while the pHEMT amplifier featured low power consumption and a gain of 24 dB over the 85-100 GHz range. A butterfly metal package equipped with a 1.0 mm (W) coaxial connector and a microstrip-coplanar waveguide conversion substrate was designed for low insertion loss and low return loss. The fabricated photoreceiver module demonstrated high conversion gain, a maximum output power of +9.5 dBm at 96 GHz, and DC-power consumption of 0.21 W.
Monitoring radiofrequency catheter ablation using thermal strain imaging (2010)

A method to monitor ablative therapy by examining slope changes in the thermal strain curve caused by speed of sound with temperature is introduced. The variation of sound speed with temperature rise for most soft tissue follows a similar pattern to that of water. Unlike most liquids, the sound speed of tissue increases with temperature. However, at temperatures above about 50°C, there is no further increase in the sound speed and the temperature coefficient may become slightly negative. For ablation therapy, an irreversible injury to tissue and a complete heart block occurs in the range of 48-50°C for a short period in accordance with the well known Arrhenius equation. Using these two properties, we propose a potential tool to detect the moment when tissue damage occurs using the reduced slope in the thermal strain curve as a function of heating time. Using a prototype intracardiac echocardiography (ICE) array for imaging and a catheter for RF ablation, we were able to observe an obvious slope change in the thermal strain curve in an excised tissue sample. The method was further tested in-vivo, using a specially equipped ablation tip and an 11 MHz microlinear (ML) ICE array mounted on the tip of a catheter. As with in-vitro experiments, the thermal strain curve showed a plateau and a change in the sign of the slope.
Enhancing the Spatial Resolution for Wire Fault Detection Systems Using Multi-Carrier Signals (2018)

Recently, the world is witnessing a vast daily development in technology accompanied with more and more complex electronic systems. They are hosting cumulated lengths of electrical cables that are subject one day or another to the occurrence of wiring faults. Accordingly, wire diagnosis became essentially important for ensuring safety, security, integrity, and optimal performance. On the other hand, the emergence of sensor networks and connected objects has created the need for embedded and non invasive fault diagnosis solutions. Notably, multi-carrier reflectometry methods have shown promising and efficient results in locating upcoming defects on wires in an online manner. Nevertheless, their precision stays within the physical limits of their components, especially the sampling frequency of their analog/digital transitions. In this paper, we will propose several approaches combining multi-carrier reflectometry with phase analysis techniques to overcome this limit. Accordingly, an improvement in the precision of the fault localization and a high spatial resolution is obtained. Based on an FPGA implementation, our novel methods and the resulting systems have proven a five-time better accuracy than the state-of-the-art methods on the same platform.
Chiral Mandelic Acid Template Provides a Highly Practical Solution for (S)-Oxybutynin Synthesis (2000)

ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTChiral Mandelic Acid Template Provides a Highly Practical Solution for (S)-Oxybutynin SynthesisPaul T. Grover, Nandkumar N. Bhongle, Stephen A. Wald, and Chris H. SenanayakeView Author Information Chemical Research and Development, Sepracor Inc., 111 Locke Drive, Marlborough, Massachusetts 01752 [email protected]Cite this: J. Org. Chem. 2000, 65, 19, 6283–6287Publication Date (Web):August 22, 2000Publication History Received28 June 2000Published online22 August 2000Published inissue 1 September 2000https://pubs.acs.org/doi/10.1021/jo005562fhttps://doi.org/10.1021/jo005562fbrief-reportACS PublicationsCopyright © 2000 American Chemical SocietyRequest reuse permissionsArticle Views1346Altmetric-Citations68LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Aldol reactions,Crystallization,Lactones,Solvents,Stereoselectivity Get e-Alerts
Optical Property Changes in Low-k Films upon Ultraviolet-Assisted Curing (2008)

Ultraviolet-assisted curing (UV curing) has been applied recently to enhance the mechanical properties of low- films. Knowledge about which UV energies are most effective is still limited and the consequences of applying the UV-curing process to integrated stacks in on-chip interconnects are unknown. To clarify these open questions, we investigated the optical properties of a SiCOH low- layer by purged UV spectroscopic ellipsometry in the energy region 2–9 eV. The complex refractive index of the low- film shows an absorption edge with a superimposed absorption band at 6.4 eV that vanishes upon UV-assisted curing. A comparison with Fourier transform infrared transmission demonstrates that the absorption at 6.4 eV must be attributed to the organic porogens, which also influence the absorption edge. Further analysis reveals the redshift of the absorption edge of silica-based low- films with the presence of carbon species. The measured optical properties permit simulation of the standing wave pattern of light within the films in differently configured stacks.
Quebec platelet disorder is linked to the urokinase plasminogen activator gene (PLAU) and increases expression of the linked allele in megakaryocytes (2008)

Quebec platelet disorder (QPD) is an autosomal dominant disorder with high penetrance that is associated with increased risks for bleeding. The hallmark of QPD is a gain-of-function defect in fibrinolysis due to increased platelet content of urokinase plasminogen activator (uPA) without systemic fibrinolysis. We hypothesized that increased expression of uPA by differentiating QPD megakaryocytes is linked to PLAU. Genetic marker analyses indicated that QPD was significantly linked to a 2-Mb region on chromosome 10q containing PLAU with a maximum multipoint logarithm of the odds (LOD) score of +11 between markers D10S1432 and D10S1136. Analysis of PLAU by sequencing and Southern blotting excluded mutations within PLAU and its known regulatory elements as the cause of QPD. Analyses of uPA mRNA indicated that QPD distinctly increased transcript levels of the linked PLAU allele with megakaryocyte differentiation. These findings implicate a mutation in an uncharacterized cis element near PLAU as the cause of QPD.
Effect of Supercritical Carbon Dioxide on the Loading and Release of Model Drugs from Polyurethane Films: Comparison with Solvent Casting (2013)

Thermoplastic polyurethanes (TPUs) are widely used in biomedical applications because of their excellent mechanical properties and biocompatibility. However, their role as possible matrices for drug delivery has received little attention. In this study, the loading and release of model drugs from different TPUs using supercritical CO 2 (scCO 2 ) as an inert and convenient solvent are investigated. The effects of scCO 2 treatment on the physical properties of ElastEon TPU films are assessed. The results indicate subtle effects on the microphase morphology of the TPU without significant changes in its mechanical properties. Model drugs (rhodamine B, fluorescein, and 7‐hydroxycoumain) are readily loaded into the films and show homogeneous impregnation across the thickness of the film. The loading capacity under the conditions used in the present study varies from less than 1% for rhodamine B to approximately 4% for 7‐hydroxycoumarin. The release kinetics not only vary between the different drugs, but are also altered by changes in the composition of the hard and soft segments of the TPUs. These results demonstrate the potential of scCO 2 treatment in preparing drug‐loaded poly­urethanes used in medical devices. This information may be helpful in designing TPUs with different drug‐release profiles for a variety of disease treatments. image
Transmission of real-time video over IP differentiated services (2002)

Multimedia applications require high bandwidth and guaranteed quality of service (QoS). The current Internet, which provides ‘best effort’ services, cannot meet the stringent QoS requirements for delivering MPEG videos. It is proposed that MPEG frames are transported through various service models of DiffServ. Performance analysis and simulation results show that the proposed approach can not only guarantee QoS but can also achieve high bandwidth utilisation.
Crystal and molecular structure of (23R)-23-hydroxy-3α,5α-cycloergost-7-en-6-one (1973)

The title compound (III) crystallises in the monoclinic space group P21, with cell dimensions a= 8·048, b= 20·122, c= 7·641 Å, β= 93·92°, and Z= 2. The structure has been solved by direct methods, and refined to R 0·0845 for 1777 significant reflections, measured on a diffractometer. The configuration at C(23) is R, thus confirming the previous assignment. The conformation of the steroid nucleus is of some interest; whereas the BC and CD ring junctions are trans, AB is cis due to the C(3)–C(4)–C(5) cyclopropane ring. Rings A and B, and to a small extent, C, are subject to steric strains due to both the bicyclo[3.1.0]hexane ring A and the enone group in ring B. This is reflected in abnormal values for some bond lengths, angles, and dihedral angles.
Implicit Memory in Monkeys: Development of a Delay Eyeblink Conditioning System with Parallel Electromyographic and High-Speed Video Measurements (2015)

Delay eyeblink conditioning, a cerebellum-dependent learning paradigm, has been applied to various mammalian species but not yet to monkeys. We therefore developed an accurate measuring system that we believe is the first system suitable for delay eyeblink conditioning in a monkey species (Macaca mulatta). Monkey eyeblinking was simultaneously monitored by orbicularis oculi electromyographic (OO-EMG) measurements and a high-speed camera-based tracking system built around a 1-kHz CMOS image sensor. A 1-kHz tone was the conditioned stimulus (CS), while an air puff (0.02 MPa) was the unconditioned stimulus. EMG analysis showed that the monkeys exhibited a conditioned response (CR) incidence of more than 60% of trials during the 5-day acquisition phase and an extinguished CR during the 2-day extinction phase. The camera system yielded similar results. Hence, we conclude that both methods are effective in evaluating monkey eyeblink conditioning. This system incorporating two different measuring principles enabled us to elucidate the relationship between the actual presence of eyelid closure and OO-EMG activity. An interesting finding permitted by the new system was that the monkeys frequently exhibited obvious CRs even when they produced visible facial signs of drowsiness or microsleep. Indeed, the probability of observing a CR in a given trial was not influenced by whether the monkeys closed their eyelids just before CS onset, suggesting that this memory could be expressed independently of wakefulness. This work presents a novel system for cognitive assessment in monkeys that will be useful for elucidating the neural mechanisms of implicit learning in nonhuman primates.
China's symbolic plight and breakthrough——Another investigation into the poetics system in China (2006)

Philosophers in ancient China early realized that a semantic triangle composed of symbol,expositor,and object was not a perfect expression system: symbolic system whose perceptive and physical attributes break away from object can not completely denote the object per se.Some objects such as Dao,Xuan etc,which signify ontology of object,are hard to be thoroughly denoted due to their universality and supra-temporality.In the history of philosophy in China,many philosophical schools including confucian,Daoist, Moist,Faist,Mingist,zaiist,and buddhist looked at symbolic plight from their own standpoints,and over a long debate a special intersection mode had been engendered,leading to a breakthrough of China's symbol from plight.In addition, the propositions such as Xing-Ming,Wu-Wo,Yan-Yi in the China's symbolic intersection mode,are important issues in China's poetics.
The Fundamentals for Mobile Broadband, HSPA Evolution (2015)

This book is essential reading for those wishing to obtain a systems perspective and a broad view on the background, performance and application of the latest developments in HSPA in the context of the demands on todays mobile broadband devices and networks. It takes the reader behind the scenes of 3GPP and provides an easily accessible understanding of the basic principles, the latest steps in the standards evolution, and the motivations behind the development of standardized features. It covers important topics such as smartphone related features, multi-carrier and multi-antenna operation, interface architecture, heterogeneous networks and HSPA system performance. Offering full coverage of recent developments in HSPA up to Release 13, this book will provide a comprehensive description of one of the dominating standards for mobile broadband.
Incorporating Plutonium Particle Size Effects in the Assessment of Active Mode Measurement Uncertainty in Passive-Active Neutron Radioassay Systems (2002)

Assessment of active mode measurement uncertainty in passive-active neutron radioassay systems used to measure Pu content in nuclear waste is severely hampered by lack of knowledge of the waste Pu particle size distribution, which is a major factor in determining bias in active mode measurements. The sensitivity of active mode measurements to particle size precludes using simulations or surrogate waste forms to estimate uncertainty in active mode measurements when the particle size distribution is not precisely known or inadequately reproduced. An alternative approach is based on a statistical comparison of active and passive mode results in the mass range for which both active and passive mode analyses produce useable measurements. Because passive mode measurements are not particularly sensitive to particle size effects, their uncertainty can be more easily assessed. Once bias corrected, passive mode measurements can serve as confirmatory measurements for the estimation of active mode bias. Further statistical analysis of the errors in measurements leads to precision estimates for the active mode.
Kinetic Resolution of Racemic 2‐Hydroxyarylketones by Asymmetric Esterification: Investigation of the Influence of the Co‐Base on the Selectivity (2020)

Abstract An efficient kinetic resolution of a variety of racemic 2‐hydroxyarylketone derivatives was developed by asymmetric esterification protocol using diphenylacetic acid, pivalic anhydride, and a catalytic amount of ( R )‐benzotetramisole (( R )‐BTM). It was revealed that employing a tertiary amine as a co‐base in this reaction is not necessary to achieve high s ‐values. All conducted reactions proceeded smoothly with good s ‐values under the optimized reaction conditions, irrespective of the steric and electronic nature of the substituents on the aromatic rings of the substrates. The most stable transition state of the reaction was determined by density functional theory (DFT) calculations.
REDUCING APNEIC PERIOD WHEN LEARNING FIBEROPTIC ENDOSCOPY (1998)

S187 Introduction: As trainees begin to learn fiberoptic endoscopy in anesthetized patients, delays in intubation may occur. [1,2] If muscle relaxants are used, a significant period of apnea is thus possible. We studied the extent to which the laryngeal mask airway (LMA), used as an aid to fiberoptic training, could help reduce this period of apnea. Methods: The investigation was approved by South Birmingham Research Ethics Committee and patients gave informed, written consent. After practice on a bronchial tree model, 16 trainees were randomly allocated to receive fiberoptic training, either with (LMA group) or without (control group) the aid of the LMA, for their first 5 nasotracheal intubations, in patients scheduled for oral surgery. Patients were anesthetized with glycopyrrolate, fentanyl, propofol, atracurium, oxygen and isoflurane. In the LMA group, the LMA was inserted and the patient's lungs were ventilated mechanically. With the videoendoscope, under supervision, trainees inspected both nostrils, selected the most patent, moved the instrument forward to the nasopharynx and identified the LMA (stage 1). The LMA was then removed and jaw thrust applied (stage 2). The fiberscope tip was then advanced to the carina (stage 3). Finally, the tracheal tube was railroaded over the endoscope to complete the intubation. Trainees in the control group also selected the most patent nostril and performed nasotracheal videoendoscopy, followed by intubation, in an orthodox manner, without using the LMA, in five apneic patients. Results were analysed using two-way ANOVA with replicated data. Results: The mean times (SDs), in seconds, for the three stages of the five endoscopies in the LMA group, are shown in Table 1:Table 1Hence, apncic endoscopy time = stages 2 + 3 and total endoscopy time = stages 1 + 2 + 3. These mean times (SDs), in seconds, were compared with mean endoscopy times in the control group: (Table 2)Table 2Though total endoscopy time in the LMA group, on average, was significantly longer, (145 s. v. 114 s.; p<0.001) apneic time was significantly shorter, (60 s. v. 114 s.; p<10-8) than endoscopy time (apneic) in the control group. Discussion: This technique may therefore have a useful role to play in ensuring patient safety during early fiberoptic videoendoscopy training with apneic, anesthetized patients.
Lack of p53 Augments Antitumor Functions in Cytolytic T Cells (2016)

Repetitive stimulation of T-cell receptor (TCR) with cognate antigen results in robust proliferation and expansion of the T cells, and also imprints them with replicative senescence signatures. Our previous studies have shown that life-span and antitumor function of T cells can be enhanced by inhibiting reactive oxygen species (ROS) or intervening with ROS-dependent JNK activation that leads to its activation-induced cell death. Because tumor suppressor protein p53 is also a redox active transcription factor that regulates cellular ROS generation that triggers downstream factor-mediating apoptosis, we determined if p53 levels could influence persistence and function of tumor-reactive T cells. Using h3T TCR transgenic mice, with human tyrosinase epitope-reactive T cells developed on p53 knockout (KO) background, we determined its role in regulating antitumor T-cell function. Our data show that as compared with h3T cells, h3T-p53 KO T cells exhibited enhanced glycolytic commitment that correlated with increased proliferation, IFNγ secretion, cytolytic capacity, expression of stemness gene signature, and decreased TGF-β signaling. This increased effector function correlated to the improved control of subcutaneously established murine melanoma after adoptive transfer of p53-KO T cells. Pharmacological inhibition of human TCR-transduced T cells using a combination of p53 inhibitors also potentiated the T-cell effector function and improved persistence. Thus, our data highlight the key role of p53 in regulating the tumor-reactive T-cell response and that targeting this pathway could have potential translational significance in adoptive T-cell therapy. Cancer Res; 76(18); 5229-40. ©2016 AACR.