gebhart/patrepeval huggingface dataset:
Phase I Study of Multiple Epitope Peptide Vaccination in Patients With Recurrent or Persistent Cervical Cancer (2018)

Cancer immunotherapy has now been established as a leading standard therapeutic option in a subset of patients with cancer. In this study, we conducted a phase I dose-escalation trial using a mixture of 5 peptides to vaccinate cervical cancer patients with HLA-A*2402. The primary endpoints were safety and determination of a recommended vaccine dose, and the secondary endpoints were evaluations of immunologic responses and clinical efficacy. All patients had recurrent or persistent disease and had failed to respond to or were intolerant to prior standard chemotherapy. Peptides derived from forkhead box protein M1 (FOXM1), maternal embryonic leucine zipper kinase (MELK), Holliday junction-recognition protein, and vascular endothelial growth factor receptors 1 and 2 were administered to 9 patients in a 3 patient-cohort design, with doses of 0.5, 1, or 2 mg of each of the individual peptides in a mixture with incomplete Freund's adjuvant. The major adverse events were anemia and injection site reactions, which were seen in 77.8% (7/9) and 66.7% (6/9) of patients, respectively. Grade 3 anemia was observed in 1 patient. No dose-limiting toxicity of the vaccine was observed. Seven (78%) patients achieved stable disease, and the median progression-free survival was 3.3 months (102 d). Interferon-γ enzyme-linked immunospot assays for each of the 5 antigens showed that 8 (89%) and 7 (78%) patients had high T-cell responses to FOXM1 and MELK, respectively. In conclusion, we demonstrated that this 5-peptide vaccine was tolerable, and that FOXM1 and MELK could be promising targets for immunotherapy in patients with cervical cancer.
GISAXS study of directed self-assembly of block copolymers (2012)

International audience
Induction of Protective Serum Meningococcal Bactericidal and Diphtheria-Neutralizing Antibodies and Mucosal Immunoglobulin A in Volunteers by Nasal Insufflations of theNeisseria meningitidisSerogroup C Polysaccharide-CRM197 Conjugate Vaccine Mixed with Chitosan (2005)

Thirty-six healthy volunteers received either a single intramuscular injection of Neisseria meningitidis serogroup C polysaccharide (MCP)-CRM197 conjugate vaccine in alum or two nasal insufflations 28 days apart of the same vaccine powder, without alum, mixed with chitosan. Nasal immunization was well tolerated, with fewer symptoms reported than after intramuscular injection. The geometric mean concentrations of MCP-specific immunoglobulin G (IgG) after one nasal immunization were 3.25 microg/ml in naïve subjects and 14.4 microg/ml in subjects previously immunized parenterally, compared with 4.30 microg/ml in naïve subjects immunized intramuscularly. The geometric mean titer of serum bactericidal antibody (SBA) rose 24-fold after two nasal immunizations in naïve subjects and was comparable to parenteral immunization (1,080 versus 1,625). All subjects achieved SBA titers associated with protection after two nasal immunizations: even those with titers of <8 at entry. A single nasal immunization boosted the SBA titer to > or =128 in 96% of previously immunized subjects, and two immunizations achieved this level in 92% of naive subjects. MCP-specific IgG levels were approximately 70% IgG2 and approximately 20% IgG1 after nasal or intramuscular immunization. Increases in CRM197-specific IgG and diphtheria toxin-neutralizing activity were observed after nasal or intramuscular immunization, with balanced IgG1/IgG2 and higher IgG4. Significant MCP-specific secretory IgA was detected in nasal wash only after nasal immunization and predominantly on the immunized side. Simple nasal insufflation of existing MCP-CRM197 conjugate vaccines in chitosan offers an inexpensive but effective needle-free prime and boost against serogroup C N. meningitidis and diphtheria.
High-Quality Microprintable and Stretchable Conductors for High-Performance 5G Wireless Communication (2022)

With the advent of 5G wireless and Internet of Things technologies, flexible and stretchable printed circuit boards (PCBs) should be designed to address all the specifications necessary to receive signal transmissions, maintaining the signal integrity, and providing electrical connections. Here, we propose a silver nanoparticle (AgNP)/silver nanowire (AgNW) hybrid conductor and high-quality microprinting technology for fabricating flexible and stretchable PCBs in high-performance 5G wireless communication. A simple and low-cost reverse offset printing technique using a commercial adhesive hand-roller was adapted to ensure high-resolution and excellent pattern quality. The AgNP/AgNW micropatterns were fabricated in various line widths, from 5 μm to 5 mm. They exhibited excellent pattern qualities, such as fine line spacing, clear edge definition and outstanding pattern uniformity. After annealing via intense pulsed light irradiation, they showed outstanding electrical resistivity (15.7 μΩ cm). Moreover, they could withstand stretching up to a strain of 90% with a small change in resistance. As a demonstration of their practical application, the AgNP/AgNW micropatterns were used to fabricate 5G communication antennas that exhibited excellent wireless signal processing at operating frequencies in the C-band (4-8 GHz). Finally, a wearable sensor fabricated with these AgNP/AgNW micropatterns could successfully detected fine finger movements in real time with excellent sensitivity.
Low-Intensity Ultrasound Stimulates Proteoglycan Synthesis in Rat Chondrocytes by Increasing Aggrecan Gene Expression (2000)

We evaluated the effect of low intensity-pulsed ultrasound stimulation on rat chondrocytes in vitro using two different 1.0-MHz ultrasound signals with spatial and temporal average intensities of 50 or 120 mW/cm2. The pulses had a duration of 200 microseconds and were repeated every millisecond, with corresponding average peak-pressure amplitudes of 230 or 360 kPa, respectively. Cells were stimulated one, three, or five times for 10 minutes each day starting the third day after plating. One group of cells was exposed to sham ultrasound as a control. The cultures were evaluated for cell proliferation (by [3H]thymidine incorporation and DNA measurement), steady-state mRNA levels of alpha1(I) and alpha1(II) procollagens and aggrecan (by Northern blotting), and proteoglycan synthesis (by [35S]sulfate incorporation). The results revealed that ultrasound causes increases in the level of aggrecan mRNA (p < 0.05) and in proteoglycan synthesis (p < 0.03) after three and five treatments. Expression of mRNA for alpha1(II) procollagen increased over time, but ultrasound had no stimulatory effect. Expression of mRNA for alpha1(I) procollagen was initially low and remained unchanged with time. Although cell proliferation increased with time in both groups, there was no statistically significant difference between the cultures treated with ultrasound and the controls (p = 0.1). The in vitro results support our previous in vivo findings that low-intensity ultrasound stimulates aggrecan mRNA expression and proteoglycan synthesis by chondrocytes, which may explain the role of ultrasound in advancing endochondral ossification, increasing the mechanical strength of fractures, and facilitating fracture repair.
Mycobacterial infections and vole bacillus vaccines (2014)

INTRODUCTION: Pregnancy is a crucial period which ultimately directly impacts two individuals health and wellbeing. Within the UK, a standardised pattern of care is established with collaborations across disciplines to the benefit of women and babies. During the COVID19 pandemic, this pattern of care was disrupted to align with protective protocols which until now, has not been formally reported. METHODS: A retrospective, mixed methods study of UK based women pregnant between the years 2012 and 2022 inclusive with no known complications was conducted to collate opinions and experiences of pregnancy with and without the impact of COVID19 restrictions. Quantitative results were analysed using the statistical package GraphPad Prism 9.2.0 and presented as mean values +/- standard deviation were appropriate. In addition, we used a phased approach to open ended questions. RESULTS: Our results showed no significant difference in either the number of appointments or the time of first appointment however an increased percentage of women reported the use of private services during the COVID pandemic. There was no change in the number of midwife appointments during the postnatal period during COVID but there was a significant reduction in the number of health visitor appointments. Overall, the COVID pandemic led to women feeling less satisfied with their care both during their pregnancy and postnatally, but they reported that they continued to be listened to and remained feeling in control of their pregnancy. DISCUSSION: Generally, the changes implemented during the COVID pandemic did not impact women’s pregnancy journey substantially although we have no evidence of the long-term impact on child health and development. Clear themes have been established which can be used to further improve services in maternity and there are key elements to focus on for the future of UK maternity services.
Fluorescence technologies for the investigation of chemical libraries (2006)

This is Chapter 10 of the book Exploiting Chemical Diversity for Drug Discovery which forms part of the RSC Biomolecular Sciences series. More information about this book and the whole series is available from www.rsc.org/biomolecularsciences or the RSC Sales team (sales@rsc.org).
Posterior Cervical Foraminotomy for Radiculopathy (2017)

Shifflett, Grant D. MD; Mayo, Benjamin C. BA; Massel, Dustin H. BS; Phillips, Frank M. MD Author Information
Effects of n -Butanol Blends on the Formation of Hydrocarbons and PAHs from Fuel-Rich Heptane Combustion in a Micro Flow Reactor with a Controlled Temperature Profile (2020)

The effects of the addition of n-butanol on the formation of hydrocarbons and polycyclic aromatic hydrocarbons (PAHs) of n-heptane combustions were investigated using a micro flow reactor (MFR) with a controlled temperature profile. The concentrations of small and large hydrocarbons, as well as PAHs were measured at a maximum wall temperature of 1,100 K and atmospheric pressure. The values obtained from several mechanisms were compared to the measurement values at equivalence ratios of 2.0–5.0. The CRECK mechanism was in fair agreement with the measurements. The computational results confirmed that the concentration of the PAHs and soot precursors’ decreased, while that of CO and CO2 increased due to the addition of butanol. These trends were also shown by the measurement values. The reaction path and rate of production analyzes were carried out to identify the major reactions contributing toward species concentrations.
Propagation of <em>Homalodisca coagulata virus-01</em> via <em>Homalodisca vitripennis</em> Cell Culture (2014)

The glassy-winged sharpshooter (Homalodisca vitripennis) is a highly vagile and polyphagous insect found throughout the southwestern United States. These insects are the predominant vectors of Xylella fastidiosa (X. fastidiosa), a xylem-limited bacterium that is the causal agent of Pierce's disease (PD) of grapevine. Pierce’s disease is economically damaging; thus, H. vitripennis have become a target for pathogen management strategies. A dicistrovirus identified as Homalodisca coagulata virus-01 (HoCV-01) has been associated with an increased mortality in H. vitripennis populations. Because a host cell is required for HoCV-01 replication, cell culture provides a uniform environment for targeted replication that is logistically and economically valuable for biopesticide production. In this study, a system for large-scale propagation of H. vitripennis cells via tissue culture was developed, providing a viral replication mechanism. HoCV-01 was extracted from whole body insects and used to inoculate cultured H. vitripennis cells at varying levels. The culture medium was removed every 24 hr for 168 hr, RNA extracted and analyzed with qRT-PCR. Cells were stained with trypan blue and counted to quantify cell survivability using light microscopy. Whole virus particles were extracted up to 96 hr after infection, which was the time point determined to be before total cell culture collapse occurred. Cells were also subjected to fluorescent staining and viewed using confocal microscopy to investigate viral activity on F-actin attachment and nuclei integrity. The conclusion of this study is that H. vitripennis cells are capable of being cultured and used for mass production of HoCV-01 at a suitable level to allow production of a biopesticide.
Clinical and virological aspects of HIV2 infection in rhesus monkeys. (1992)

To establish an animal model of AIDS, two different "wild" or "adapted" HIV2 Rod and Eho strains were cultivated on monkey cells from different species (baboons, cynomolgus, Rhesus monkeys). Five different available strains were then injected both by intravenous (i.v.) and intracerebral (i.c.) route into ten Rhesus monkeys. Seven animals seroconverted between days 13 and 230. Reverse transcriptase activity in the lymphocyte culture supernatants was detectable in six of the seven animals that seroconverted, and in one animal that remained seronegative. Lymphopenia and a decrease in the CD4+ cell counts were observed in eight animals. One animal, inoculated with HIV2-Rod "wild type," developed a severe cachexia, with dyspnea, and associated neurological symptoms 150 days after inoculation. This animal was sacrificed on day 220. Pathological examination showed typical lesions of actinomycetes infection in the lungs and in the meninges. Another monkey had significant weight loss associated with lymphadenopathies and pancytopenia. These results suggest that in vivo replication of HIV2 in Rhesus monkeys may induce clinical symptoms of immune deficiency. This method is reproducible and may provide a good model for AIDS.
Tunable Compact Low-Noise Amplifier With Permalloy Thin-Film Enabled Slow-Wave Transmission Lines (2015)

This paper provides a first tunable low-noise amplifier (LNA) design with Permalloy (Py) enabled inductors. Tunable inductor is an important passive device in adaptive radio frequency circuit design. In this paper, a new integrated tunable inductor is implemented utilizing novel slow-wave transmission lines that are built with coplanar waveguide having alternative high and low impedance signal sections, and the Py thin films are deposited and patterned on the signal line of narrow section to provide higher and tunable inductance density. The inductance per unit length has been increased from 877 to 949 nH/m with 100 nm Py thin film at 5 GHz. A tunable 5 GHz LNA utilizing SWLTs is developed, where the tunability of power reflection and power gain of LNA are demonstrated with variable inductors.
Solved problems, unsolved problems and non-problems in concurrency (1985)

This is an edited transcript of a talk given at last year's conference. To preserve the flavor of the talk and the questions, I have done very little editing---mostly eliminating superfluous words and phrases, correcting especially atrocious grammar, and making the obvious changes needed when replacing slides by figures. The tape recorder was not functioning for the first few minutes, so I had to recreate the beginning of the talk.
Prospects for single-molecule detection in liquids by laser-induced fluorescence (1983)

A laser-induced fluoresence determination of aqueous solutions of rhodamine 6G resulted in a detection limit of 18 attograms, or 22,000 molecules, of rhodamine 6G. These results allow the projection to single-molecule detection with reasonable improvements in the experimental apparatus.
Data from The Pathognomonic FOXL2 C134W Mutation Alters DNA-Binding Specificity (2023)

&lt;div&gt;Abstract&lt;p&gt;The somatic missense point mutation c.402C&gt;G (p.C134W) in the FOXL2 transcription factor is pathognomonic for adult-type granulosa cell tumors (AGCT) and a diagnostic marker for this tumor type. However, the molecular consequences of this mutation and its contribution to the mechanisms of AGCT pathogenesis remain unclear. To explore these mechanisms, we engineered V5-FOXL2&lt;sup&gt;WT&lt;/sup&gt;- and V5-FOXL2&lt;sup&gt;C134W&lt;/sup&gt;–inducible isogenic cell lines and performed chromatin immunoprecipitation sequencing and transcriptome profiling. FOXL2&lt;sup&gt;C134W&lt;/sup&gt; associated with the majority of the FOXL2 wild-type DNA elements as well as a large collection of unique elements genome wide. This model enabled confirmation of altered DNA-binding specificity for FOXL2&lt;sup&gt;C134W&lt;/sup&gt; and identification of unique targets of FOXL2&lt;sup&gt;C134W&lt;/sup&gt; including &lt;i&gt;SLC35F2&lt;/i&gt;, whose expression increased sensitivity to YM155. Our results suggest FOXL2&lt;sup&gt;C134W&lt;/sup&gt; drives AGCT by altering the binding affinity of FOXL2-containing complexes to engage an oncogenic transcriptional program.&lt;/p&gt;Significance:&lt;p&gt;A mechanistic understanding of FOXL2&lt;sup&gt;C134W&lt;/sup&gt;-induced regulatory state alterations drives discovery of a rationally designed therapeutic strategy.&lt;/p&gt;&lt;/div&gt;
Quieting a Rib-Framed Honeycomb Core Sandwich Panel for a Rotorcraft Roof (2017)

A rotorcraft roof composite sandwich panel has been redesigned to optimize sound power transmission loss (TL) and minimize structure-borne sound for frequencies between 1 and 4 kHz where gear meshing noise from the transmission has the most impact on speech intelligibility. The roof section, framed by a grid of ribs, was originally constructed of a single honeycomb core/composite facesheet sandwich panel. The original panel has acoustic coincidence frequencies near 600 Hz, leading to poor TL across the frequency range of 1 to 4 kHz. To quiet the panel, the cross section was split into two thinner sandwich subpanels separated by an air gap. The air gap was sized to target the fundamental mass-spring-mass resonance of the panel system to less than 500 Hz, well below the frequency range of interest. The panels were designed to withstand structural loading from normal rotorcraft operation, as well as 'man-on-the-roof' static loads experienced during maintenance operations. Thin layers of viscoelastomer were included in the facesheet ply layups, increasing panel damping loss factors from about 0.01 to 0.05. Transmission loss measurements show the optimized panel provides 6-11 dB of acoustic transmission loss improvement, and 6-15 dB of structure-borne sound reduction at critical rotorcraft transmission tonal frequencies. Analytic panel TL theory simulates the measured performance within 3 dB over most frequencies. Detailed finite element (FE)/boundary element (BE) modeling simulates TL slightly more accurately, within 2 dB for frequencies up to 4 kHz, and also simulates structure-borne sound well, generally within 3 dB.
Using capillary electrophoresis to follow the acetylation of the amino groups of insulin and to estimate their basicities (1995)

Capillary electrophoresis (CE) is an analytical method that is useful for investigating processes that modify the charge of proteins. This paper explores the ability of CE to rationalize charges and electrophoretic mobilities of a simple protein--insulin and its acylated derivatives--as a function of pH. Insulin is a peptide hormone (MW = 5700) that has two alpha-amino groups (G alpha and F alpha) and one epsilon-amino group (K epsilon). Treatment of insulin with acetic anhydride affords seven derivatives that differ in the sites of acetylation of the three amino groups. Analysis of the pH dependence of the electrophoretic mobilities of these derivatives gives pKa values for the two N-terminal ammonium groups: pKa (G alpha) = 8.4; pKa (F alpha) = 7.1. Values of the total charge of insulin estimated from electrophoretic mobility differ from those estimated from values of pKa for its ionizable groups by less than 0.5 unit for both bovine and human insulins over the range of pH from 5.5 to 9.5. Analysis of the concentration dependence of the electrophoretic mobility of insulin yields a lower limit for the association constant for dimerization of insulin of KD > or = 6 x 10(3) M-1 (25 mM tris and 192 mM Gly, pH 8.4). Studies of electrophoretic mobility as a function of pH and extent of acetylation of amino groups rationalize the charge of insulin in detail. The sensitivity of CE to charge permits the quantitative study of electrostatic properties of proteins in solution. Insulin is a useful small-protein model with which to investigate phenomena in electrophoresis.
Table S1. Common upstream regulators. from Proteasomal Inhibition by Ixazomib Induces CHK1 and MYC-Dependent Cell Death in T-cell and Hodgkin Lymphoma (2023)

&lt;p&gt;A. Common upstream regulators predicted to be activated or inhibited (as indicated by the activation Z-score) in the ixazomib 25nM/24 hours versus control for Jurkat, L540 and L428 cells obtained through the use of Ingenuity Pathway Analysis (IPA) software. Regulation z-score indicates the degree of inhibition (for negative values) or activation (for positive values). The third column denotes the effects that the upstream regulators have on tumor progression (reference in parenthesis). B. Common upstream regulators predicted to be activated or inhibited (as described in Table S1A), are highlighted (shaded in red) based on their functional link in "hallmarks of cancer," with literature evidences listed.&lt;/p&gt;
Bariatric Surgery Restores Cardiac and Sudomotor Autonomic C-Fiber Dysfunction towards Normal in Obese Subjects with Type 2 Diabetes (2016)

This study shows that bariatric surgery can restore both cardiac and sudomotor autonomic C-fiber dysfunction in subjects with diabetes, potentially impacting morbidity and mortality.
[INVITED] Superconductor Electronics Technology Roadmap for IRDS 2018 (2018)

oral presentation - 5EOr1A-01
DOTS Secure Session Layer Services (2016)

This document describes using a session layer service for DOTS
messaging to provide secure messaging while delivering on a number of
DOTS requirements including avoiding fate-sharing with the under-
lying communications.
Textured digital elevation model formation from low-cost UAV ladar/digital image data (2015)

Textured digital elevation models (TDEMs) have valuable use in precision agriculture, situational awareness, and disaster response. However, scientific-quality models are expensive to obtain using conventional aircraft-based methods. The cost of creating an accurate textured terrain model can be reduced by using a low-cost (&lt;$20k) UAV system fitted with ladar and electro-optical (EO) sensors. A texel camera fuses calibrated ladar and EO data upon simultaneous capture, creating a texel image. This eliminates the problem of fusing the data in a post-processing step and enables both 2D- and 3D-image registration techniques to be used. This paper describes formation of TDEMs using simulated data from a small UAV gathering swaths of texel images of the terrain below. Being a low-cost UAV, only a coarse knowledge of position and attitude is known, and thus both 2D- and 3D-image registration techniques must be used to register adjacent swaths of texel imagery to create a TDEM. The process of creating an aggregate texel image (a TDEM) from many smaller texel image swaths is de- scribed. The algorithm is seeded with the rough estimate of position and attitude of each capture. Details such as the required amount of texel image overlap, registration models, simulated flight patterns (level and turbulent), and texture image formation are presented. In addition, examples of such TDEMs are shown and analyzed for accuracy.