gebhart/patrepeval huggingface dataset:
Monte Carlo economic analysis of Baker's yeast invertase purification using two‐ and three‐phase partitioning (2018)

Abstract BACKGROUND Invertase use in the food industry is limited by production costs. Alternative strategies for extraction, such as aqueous two‐phase systems (ATPS) and three‐phase partitioning (TPP), could be economically feasible for yeast invertase. Economic modeling of bioprocesses makes possible the identification of critical parameters for production costs and emulation of real scenarios, moreover incorporation of uncertainty is possible. This study performed an economic analysis on the production of invertase using ATPS or TPP, also a virtual optimization of ATPS was done. RESULTS TPP provided a lower production cost than ATPS ($145 vs $59.3 per 1 million enzymatic units, respectively). The critical parameter for TPP is recovery yield as it is highly dependent on operating conditions. In contrast, ATPS is dependent on materials costs as the sample load is smaller for ATPS, requiring a larger system. Although TPP provided a lower cost, t‐butanol hinders its acceptance. CONCLUSION Virtual optimization of ATPS found that varying sample load or system size is not enough to have a lower production cost than TPP, but provided insights for reduction of production costs and development of a safer technique. This study provides a framework for the virtual analysis of ATPS and TPP to evaluate their processes and reduce production costs. © 2018 Society of Chemical Industry
Prostaglandin Biosynthesis, Transport, and Signaling in Corpus Luteum: A Basis for Autoregulation of Luteal Function (2004)

The corpus luteum (CL) is a transient ovarian endocrine gland formed from the ovulated follicle. Progesterone is the primary secretory product of CL and is essential for establishment of pregnancy in mammals. In the cyclic female, the life span of CL is characterized by luteal development, maintenance, and regression regulated by complex interactions between luteotrophic and luteolytic mediators. It is universally accepted that prostaglandin (PG) F(2a) is the luteolysin whereas PGE(2) is considered as a luteotropin in most mammals. New emerging concepts emphasize the autocrine and paracrine actions of luteal PGs in CL function. However, there is no report on selective biosynthesis and cellular transport of luteal PGE(2) and PGF(2alpha) in the CL of any species. We have studied the expression of enzymes involved in the metabolism of PGE(2) and PGF(2alpha), cyclooxygenase (COX)-1 and -2, PGE and F synthases, PG 15-dehydrogenase, and PG transporter as well as receptors (EP2, EP3, and FP) throughout the CL life span using a bovine model. COX-1, PGF synthase, and PG 15-dehydrogenase are expressed at constant levels whereas COX-2, PGE synthase, PG transporter, EP2, EP3, and FP are highly modulated during different phases of the CL life span. The PG components are preferentially expressed in large luteal cells. The results indicate that PGE(2) biosynthesis, transport, and signaling cascades are selectively activated during luteal maintenance. By contrast the PGF(2alpha) system is activated during luteal regression. Collectively, our results suggest an integrated role for luteal PGE(2) and PGF(2alpha) in autoregulation of CL function.
Solutions de stockage de l'énergie pour les systèmes de production intermittente d'électricité renouvelable (2004)

International audience
The renal dopamine receptors. (1992)

Dopamine is an endogenous catecholamine that modulates many functions including behavior, movement, nerve conduction, hormone synthesis and release, blood pressure, and ion fluxes. Dopamine receptors in the brain have been classically divided into D1 and D2 subtypes, based on pharmacological data. However, molecular biology techniques have identified many more dopamine receptor subtypes. Several of the receptors cloned from the brain correspond to the classically described D1 and D2 receptors. Several D1 receptor subtypes have been cloned (D1A, D1B, and D5) and are each coupled to the stimulation of adenylyl cyclase. The D2 receptor has two isoforms, a shorter form, composed of 415 amino acids, is termed the D2short receptor. The long form, called the D2long receptor, is composed of 444 amino acids; both are coupled to the inhibition of adenylyl cyclase. The D3 and D4 receptors are closely related to, but clearly distinct from, the D2 receptor. They have not yet been linked to adenylyl cyclase activity. Outside of the central nervous system, the peripheral dopamine receptors have been classified into the DA1 and DA2 subtypes, on the basis of synaptic localization. The pharmacological properties of DA1 receptors roughly approximate those of D1 and D5 receptors, whereas those of DA2 receptors approximate those of D2 receptors. A renal dopamine receptor with some pharmacological features of the D2 receptor but not linked to adenylyl cyclase has been described in the renal cortex and inner medulla. In the inner medulla, this D2-like receptor, termed DA2k, is linked to stimulation of prostaglandin E2 production, apparently due to stimulation of phospholipase A2. Of the cloned dopamine receptors, only the mRNA of the D3 receptor has been reported in the kidney. The DA1 receptor in the kidney is associated with renal vasodilation and an increase in electrolyte excretion. The DA1-related vasodilation and inhibition of electrolyte transport is mediated by cAMP. The role of renal DA2 receptors remains to be clarified. Although DA1 and DA2 receptors may act in concert to decrease transport in the renal proximal convoluted tubule, the overall function of DA2 receptors may be actually the opposite of those noted for DA1 receptors. Dopamine has been postulated to act as an intrarenal natriuretic hormone. Moreover, an aberrant renal dopaminergic system may play a role in the pathogenesis of some forms of hypertension. A decreased renal production of dopamine and/or a defective transduction of the dopamine signal is/are present in some animal models of experimental hypertension as well as in some forms of human essential hypertension.
Viscoelastic modulus of agarose gels by magnetic resonance elastography using Micro-MRI (2015)

This study aimed to apply magnetic resonance elastography (MRE) using micro-magnetic resonance imaging (micro-MRI) system for the measurements of viscoelastic modulus in soft matters. The rectangular specimens of 90 × 70 × 50 mm were made of agarose gel with five kinds of stiffness by changing concentrations. The specimens were oscillated with longitudinal waves transmitted by an elastic-bar from a vibration generator in a micro-MRI system. Since the viscoelastic properties depend on the excitation frequency and amplitude, the experimental conditions were selected in the range of 50-250 Hz and 0.1-0.5 mm. The viscoelastic modulus was expressed as storage shear modulus G´ and loss shear modulus G˝. As a result, G´ increased with the frequency and amplitude, and the difference of G´ between hard and soft gels was obtained. The viscoelastic modulus of agarose gels was measured using the MRE system under the excitation conditions. Furthermore, double-layer specimens composed of 0.6 and 2.0 wt% gels were examined as an application of the MRE system. The difference of wave pattern between the hard and soft parts was observed. The values of G´ in the soft parts of the double-layer specimens corresponded to the value of the single-layer specimen, but the values of G´ in the hard parts were varied.
Synthesis and structural characterization of (.eta.4-cyclopentadienone)(.eta.5-cyclopentadienyl)dicarbonylmolybdenum hexafluorophosphate. A template for the stereospecific construction of cis-4,5-disubstituted-2-cyclopentenones (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and structural characterization of (.eta.4-cyclopentadienone)(.eta.5-cyclopentadienyl)dicarbonylmolybdenum hexafluorophosphate. A template for the stereospecific construction of cis-4,5-disubstituted-2-cyclopentenonesLanny S. Liebeskind and Agnes BombrunCite this: J. Am. Chem. Soc. 1991, 113, 23, 8736–8744Publication Date (Print):November 1, 1991Publication History Published online1 May 2002Published inissue 1 November 1991https://pubs.acs.org/doi/10.1021/ja00023a021https://doi.org/10.1021/ja00023a021research-articleACS PublicationsRequest reuse permissionsArticle Views366Altmetric-Citations41LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Injectable Diabetes Treatments Targeting Glucose Regulation and Satiety Pathways (2018)

Medicinal Chemistry Reviews is an outstanding 680 page volume providing timely and critical reviews of important topics in medicinal chemistry. It is provided free of charge to MEDI members in electronic format. Members can also order a print copy, for the cost of printing, shipping and handling
Supplementary Table S4 from Cyst Fluid Telomerase Activity Predicts the Histologic Grade of Cystic Neoplasms of the Pancreas (2023)

&lt;p&gt;Supplementary Table S4; Diagnostic performance of using telomerase activity in combination with clinical predictors of invasive cancer or high-grade dysplasia among discovery set cases&lt;/p&gt;
Arginine-mediated dissociation of single cells and cell sheets from a polystyrene culture dish (2019)

Here, we report a novel non-enzymatic cell dissociation method, based on our finding that adherent cells dissociate rapidly from the polystyrene culture dish when incubated in an l- or d-arginine-containing solution. We also demonstrate the successful detachment of confluent NIH/3T3 cell monolayers from the culture dish as a cell sheet by the addition of an arginine solution.
Design Evolution of an Interactive Robot for Therapy (2004)

This paper describes a project using telecommunication and robotics to develop a system that addresses developmental goals for children with disabilities. A user-centered design was employed to meet the needs of the end users: children with disabilities, therapists, educators, and parents. The design team developed the prototype robot system for therapy, education, and play. The robot system is an adaptable, programmable robot, designed to address a wide range of disabilities, interests, and developmental goals. The production design will include data collection and remote monitoring capabilities. The steps leading to the prototype and its evaluation are described, including identification of user needs and system requirements to satisfy these needs.
Minimizing metal etch rate pattern sensitivity in a high density plasma etcher (1997)

The variation of metal etch rate with spacing between metal lines was measured from scanning electron microscopy micrographs of TiN/Al–0.5%Cu/TiN wafers etched in a high density inductively coupled plasma metal etcher. The metal etch rate was found to depend on the spacing between metal lines, with etch rate significantly decreasing in very narrow spaces for a conventional Cl2/BCl3 chemistry. The effect of several process parameters on this etch rate dependence was studied. It was found that the dependence could be reduced significantly, and the traditional rolloff of etch rate as spacing decreases could be eliminated, by the choice of process gases. Addition of 15% CHF3 to a BCl3/Cl2 mixture resulted in a 50% reduction of the effect, and addition of both CHF3 and Ar under certain process conditions resulted in almost complete reduction or even inversion of the effect. A mechanism is proposed for this improvement: sidewall passivants like CHF3 reduce the sticking coefficient of chlorine on aluminum, boosting reactant flux to the bottom of high aspect ratio openings.
The sweet potatoIbMYB1gene as a potential visible marker for sweet potato intragenic vector system (2010)

MYB transcription factors play important roles in transcriptional regulation of many secondary metabolites including anthocyanins. We cloned the R2R3-MYB type IbMYB1 complementary DNAs from the purple-fleshed sweet potato (Ipomoea batatas L. cv Sinzami) and investigated the expression patterns of IbMYB1 gene with IbMYB1a and IbMYB1b splice variants in leaf and root tissues of various sweet potato cultivars by reverse transcription-polymerase chain reaction. The transcripts of IbMYB1 were predominantly expressed in the purple-fleshed storage roots and they were also detectable in the leaf tissues accumulating anthocyanin pigments. In addition, transcript levels of IbMYB1 gene were up-regulated by treatment with methyl jasmonate or salicylic acid in leaf and root tissues of cv. White Star. To set up the intragenic vector system in sweet potato, we first evaluated the utilization of the IbMYB1 gene as a visible selectable marker. The IbMYB1a was transiently expressed in tobacco leaves under the control of a constitutive cauliflower mosaic virus 35S promoter, a root-specific and sucrose-inducible sporamin promoter, and an oxidative stress-inducible sweet potato anionic peroxidase2 promoter. We also showed that overexpression of IbMYB1a induced massive anthocyanin pigmentation in tobacco leaves and up-regulated the transcript levels of the structural genes in anthocyanin biosynthetic pathway. Furthermore, high-performance liquid chromatography analysis revealed that the expression of IbMYB1a led to production of cyanidin as a major core molecule of anthocyanidins in tobacco leaves. These results suggest that the IbMYB1 gene can be applicable to a visible marker for sweet potato transformation with intragenic vectors, as well as the production of anthocyanin as important nutritive value in other plant species.
Antibacterial activity of flavanostilbenes against methicillin-resistant Staphylococcus aureus (1995)

Three phytochemical compounds (alopecurone A-C), flavanostilbenes which are produced by condensation between a hydroxyflavanone and a hydroxystilbene, were isolated as major components from the root of Sophora alopecuroides. They uniformly inhibited the growth of 21 strains of methicillin-resistant Staphylococcus aureus with minimum inhibitory concentrations of 3.13-6.25 micrograms ml-1.
Magnetic beads as an extraction medium for simultaneous quantification of acetaminophen and structurally related compounds in human serum (2014)

This paper describes a sample preparation method that complements a previously published liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay for acetaminophen and eight structurally-related compounds in human serum (C. Bylda, R. Thiele, U. Kobold, D.A. Volmer. Drug Test. Anal. 2014, 6, 451). The analytes (acetaminophen [APAP] + metabolites acetaminophen-glucuronide [APG], -cysteine [APC], -mercapturate [APM] and -cysteine [APC], structurally similar analogues phenacetin and p-phenetidine, as well as tricyclic antidepressants imipramine and amitryptiline) were extracted from serum using magnetized hyper-crosslinked polystyrene particles. The sample preparation protocol was developed by means of a design of experiments (DoE) statistical approach. Using three representative compounds from the analyte panel with different polarities (high, medium, and low), two screening designs were used to identify factors that exhibited significant impact on recovery of the analytes. These parameters were then optimized to permit extraction of the complete target panel exhibiting a broad range of chemical polarities. Liquid chromatographic separations were achieved by gradient elution using a pentafluorphenyl column with subsequent detection by electrospray ionization-triple quadrupole mass spectrometry in multiple reaction monitoring (MRM) mode. The method was linear over the range 0.1-100 µg/mL for APAP, APG, p-phenetidine and phenacetin, 0.03-50 µg/mL for APS, and 0.01-10 µg/mL for APM, APC, imipramine and amitriptyline, with R(2) > 0.99. The assay exhibited good precision with CVs ranging from 2 to 9% for all analytes; the accuracy was assessed by comparing two LC-MS/MS methods using a set of 68 patient samples.
ChemInform Abstract: Design, Synthesis, and Conformational Analysis of a Novel Series of HIV Protease Inhibitors. (1999)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Novel Baseband Analog Beamforming through Resistive DACs and Sigma Delta Modulators (2020)

This paper reports a novel beamforming method based on Continuous Time Sigma-Delta Modulators (CT SDM) with feedforward (FF) programmable coefficients implemented as spatial FIR filters. This new method realizes analog beamforming in baseband and allows accurate beamsteering by programming banks of resistors. We present the theoretical framework and a proof-of-concept implementation as a spatio-temporal 250MHz signal bandwidth CT SDM in 40nm CMOS for a 9-channels phased array. This paper shows that the proposed method can significantly lower the ADC SNR requirements in phased-array receivers operating in the presence of multiple interferers inside the signal frequency band. Our method can enable power-efficient hybrid multi-link beam-forming when combined with the standard digital beamforming approaches.
Disease Eradication by Vaccination (2015)

Abstract Eradication is the last step in the fight against pathogens and the cessation of their transmission. Several efficacious vaccines have contributed immensely to the reduction of incidence of many diseases, but only a few diseases are amenable to eradication. Vaccines as a health intervention offer the best economic and social value against emerging and re‐emerging diseases and represent the best option for control, elimination and eradication of infectious pathogens. Each eradication programme is different and will require its own strategies and tactics. All eradication programmes, however, require an intensive focus. In addition, a change in mindset will also be needed, especially in rich, developed countries where the dramatic reduction of the incidence of many infectious diseases due to vaccination has now dramatically reduced the perception of the risk linked to these diseases. Key Concepts Disease eradication is the ultimate goal of all prevention campaigns. Not all infectious diseases can be eradicated even if safe and effective vaccines exist. Vaccines offer the best economic and social value against emerging and re‐emerging diseases. A shift in mindset in developed countries is needed to prevent re‐emergence in those countries of infectious diseases considered under control.
Actuation of Gold-Coated Liquid Crystal Elastomers (2013)

We demonstrate that gold layers are a promising candidate for resistive heating actuation of liquid crystalline elastomers at moderate strains of around 10% and with good repeatability up to a few 10 kilocycles. These composites are easy to manufacture and have suitable resistivity to be actuated with low-voltage power supplies. The mechanical and electrical properties of such materials are investigated and the observed resistance changes are explained in terms of a percolation model.
Solvent effects on heterogeneous synthesis of cardanol-bonded cellulose thermoplastics (2016)

Focusing on a one-pot heterogeneous process, we synthesized a promising mixed cellulose ester, cardanol-bonded cellulose thermoplastic, with bonding a short side chain (acetyl) and a long one (a derivative of cardanol). In the heterogeneous process, reaction solvents are important factors in the reactivity of long and short side chain moieties on cellulose. Investigating various kinds of solvents, we found that the solvents’ affinity for cellulose was an important parameter for the reactivity of long side chain moiety, and the polarity of solvents affected the reaction ratio of long and short chain moieties. Especially, when the middle-basic type of the solvent such as N,N-dimethylformamide or N-methylpyrrolidone was used, the obtained thermoplastics had adequate degree of substitutions of long and short chain moieties, and sufficient mechanical properties (50 MPa of bending strength, and 1.4 GPa of bending modulus) to use in durable products such as electric devices in addition to general products.
P1‐241: A NOVEL RADIOLIGAND FOR ASSESSING BACE OCCUPANCY IN P‐GP KO MOUSE BRAIN (2014)

A beta-site amyloid precursor protein cleaving enzyme (BACE) is a key enzyme in generation of amyloid-β peptides that aggregate to form amyloid plaques found in Alzheimer's disease (AD) brains. Inhibition of BACE is a potential approach for the treatment of AD and a BACE radioligand could be valuable for assessing target occupancy and therapeutic responses to BACE inhibition. Mdr1a/b (P-gp 3 and 1 deficient, termed P-gp KO), FVB wild type (wt), BACE1 knockout (KO), and BACE1 wt mice, were purchased from Taconic. Mdr1a/b and FVB WT mice were 8 weeks old, while BACE1 KO and BACE1 WT mice were two years old when used in the studies. The unlabeled compounds and [3 H]Compound-A (75.3Ci/mmol) were synthesized in house. Autoradiography was performed using frozen brain slices. In vitro binding assays were done with brain membranes or homogenates. In vivo brain occupancy was performed in Mdr1a/b, FVB and BACE1 KO mice by i.p. administration of BACE inhibitor or vehicle and then followed by i.v. dosing of the radioligand. In mouse brain homogenates, [3 H]Compound-A showed binding site densities (B max) from 6.5 nM to 8.4 nM and high binding affinity (K d = 0.5 nM), yielding good binding potentials (B max/K d). In autoradiography of BACE1 wt mice, [3 H]Compound-A binding sites were widely distributed across the brain with different binding densities in various regions, and the non-displaceable binding was low and homogenous among all the brain slices. In contrast, [3 H]Compound-A failed to show specific binding in the brain slices of BACE1 KO mice. Furthermore, the in vivo brain occupancy studies demonstrated that [3 H]Compound-A binding to Mdr1a/b brains was dose-dependent and displaceable by pre-administration of a selective, brain penetrant BACE inhibitor. In FVB mouse brains, [3 H]Compound-A did not show specific brain uptake following i.v. administration of [3 H]Compound-A. These results demonstrate that [3 H]Compound-A binding is specific to BACE which is widely distributed in mouse brain. In vivo brain uptake of [3 H]-Compound-A only occurred in P-gp KO mice, not in wild type and BACE1 KO mice. Together, these data suggest that a compound with no P-gp liability could be developed into a PET tracer for BACE.