gebhart/patrepeval huggingface dataset:
Spatiotemporal response of rat visual cortex during moving stimuli using Functional Ultrasound (fUS) imaging (2016)

The spatiotemporal study of the visual system of the rodent is a challenging area that conventional functional imaging modalities, despite fundamental achievements, still struggle to study nowadays. In the other hand Functional Ultrasound (fUS) offers an unprecedented combination of spatiotemporal resolution. By coupling fUS with an adapted visual stimulation device, we mapped the vision-evoked activity in the rodent brain. We then optimized the visual-stimulus toward the spatiotemporal study of the rodent visual pathway using fUS.
Application of Electrokinetic Nanoparticle Migration in the Production of Novel Concrete-Based Composites (2008)

Abstract : Electrophoresis has been widely used to build up complex composites by infiltrating particulate phases into porous ceramics and to facilitate putting uniform mineral coatings on metal for enameling. This technique uses a potential field to move charged particles that are suspended in a fluid phase into a porous medium. Fabrication generally involves a second step that fixes the layer of particles together and bonds them to the surrounding surfaces. When electrophoresis is used to improve portland cement-based concretes or mortars, there is no easy approach to providing a densification or cementation step that will bond the resulting composite phases together. An advanced approach has been developed that uses electro-transport techniques to migrate nano-sized particles suspended in a gel or solution that can react with the matrix to produce a cementing reaction in the pore space of the mortar or concrete to form a new composite. Electrophoretic infiltration and cementation is investigated using two types of mortar and both silicate and carbonate-based cementation. Results indicate both systems can produce useful density changes in mortar.
[Establishment of a diagnostic system for allergic diseases aiming at personalized medicine--establishment of a novel ELISA system for SCCA]. (2008)

It is known that IL-13, a Th2-type cytokine, is critical for the onset of bronchial asthma, and therefore, various anti-IL-13 antagonists are now being developed as reagents for treating asthma patients. However, there is no good way to select allergic patients into who such IL-13 antagonists should be administered. We previously found that squamous cell carcinoma antigens (SCCAs) 1 and 2 were IL-13-inducible gene products in bronchial epithelial cells and that serum SCCA level was elevated in the patients of bronchial asthma and atopic dermatitis. In this study, we tried to establish specific ELISA systems for SCCA1 and SCCA2. We generated several hybridoma clones secreting rat or mouse antibodies recognizing specifically SCCA1 or SCCA2, or both. By combining two among these antibodies, we found that the ELISA systems using rat anti-SCCA2 antibody (SS14B) as the coated antibody and rat-SCCA1 antibody (SS11G) or rat-SCCA2 antibody (SS8G) as the primary antibodies showed with high sensitivities specific recognition of SCCA1 and SCCA2, respectively. These results demonstrated that we could establish the specific ELISA system for SCCA1 and SCCA2. These specific ELISA systems for SCCA1 and SCCA2 are useful to analyze the correlation between serum SCCA level and the pathogenesis of allergic diseases and have a potential to apply to enforcement of the personalized medicine for allergic diseases.
Development of a novel DNA sensing system using DNA aptamer that inhibits enzymatic activity 2 (2004)

We have developed novel DNA sensor system using the DNA aptamers for thrombin. We modified original thrombin aptamer by addition of the target DNA complementary strands to the 5' end or 3' end of aptamer. Inhibitory activity of this modified aptamer increased in the presence of target DNA. We were able to detect the target DNA by measuring thrombin activity using this aptamer without the separation of bound and free separation.
Characterization of extracellular vesicles and synthetic nanoparticles with four orthogonal single-particle analysis platforms (2020)

ABSTRACT We compared four orthogonal technologies for sizing, counting, and phenotyping of extracellular vesicles (EVs) and synthetic particles. The platforms were: single-particle interferometric reflectance imaging sensing (SP-IRIS) with fluorescence, nanoparticle tracking analysis (NTA) with fluorescence, microfluidic resistive pulse sensing (MRPS), and nanoflow cytometry measurement (NFCM). EVs from the human T lymphocyte line H9 (high CD81, low CD63) and the promonocytic line U937 (low CD81, high CD63) were separated from culture conditioned medium (CCM) by differential ultracentrifugation (dUC) or a combination of ultrafiltration (UF) and size exclusion chromatography (SEC) and characterized by transmission electron microscopy (TEM) and Western blot (WB). Mixtures of synthetic particles (silica and polystyrene spheres) with known sizes and/or concentrations were also tested. MRPS and NFCM returned similar particle counts, while NTA detected counts approximately one order of magnitude lower for EVs, but not for synthetic particles. SP-IRIS events could not be used to estimate particle concentrations. For sizing, SP-IRIS, MRPS, and NFCM returned similar size profiles, with smaller sizes predominating (per power law distribution), but with sensitivity typically dropping off below diameters of 60 nm. NTA detected a population of particles with a mode diameter greater than 100 nm. Additionally, SP-IRIS, MRPS, and NFCM were able to identify at least three of four distinct size populations in a mixture of silica or polystyrene nanoparticles. Finally, for tetraspanin phenotyping, the SP-IRIS platform in fluorescence mode was able to detect at least two markers on the same particle, while NFCM detected either CD81 or CD63. Based on the results of this study, we can draw conclusions about existing single-particle analysis capabilities that may be useful for EV biomarker development and mechanistic studies.
48.3: A 2 Inch LTPS AMOLED with an Embedded Lateral p‐i‐n Photodiode Sensors (2008)

Abstract We developed a 2 inch low temperature poly‐Si active‐matrix organic light‐emitting diode (AMOLED) with an embedded p‐i‐n photodiode. The pixels for both OLED and photodiode are designed using poly‐Si TFT and poly‐Si p‐i‐n. The lateral p‐i‐n photodiode exhibited the photo response of ∼40 dB under 100 lux. We have demonstrated the display and sensors successfully on glass. This technology can be used for AMOLED integrated with touch sensor and brightness control sensor.
Crystal Structure of Okadaic Acid Binding Protein 2.1: A Sponge Protein Implicated in Cytotoxin Accumulation (2015)

Okadaic acid (OA) is a marine polyether cytotoxin that was first isolated from the marine sponge Halichondria okadai. OA is a potent inhibitor of protein serine/threonine phosphatases (PP) 1 and 2A, and the structural basis of phosphatase inhibition has been well investigated. However, the role and mechanism of OA retention in the marine sponge have remained elusive. We have solved the crystal structure of okadaic acid binding protein 2.1 (OABP2.1) isolated from H. okadai; it has strong affinity for OA and limited sequence homology to other proteins. The structure revealed that OABP2.1 consists of two α-helical domains, with the OA molecule deeply buried inside the protein. In addition, the global fold of OABP2.1 was unexpectedly similar to that of aequorin, a jellyfish photoprotein. The presence of structural homologues suggested that, by using similar protein scaffolds, marine invertebrates have developed diverse survival systems adapted to their living environments.
Aerodynamic and Aeroacoustic Optimization of Leading-Edge Undulation of a NACA 65(12)-10 Airfoil (2021)

Experimental studies of a NACA 65(12)-10 airfoil with a sinusoidal leading-edge undulation (LEU) were carried out to simultaneously optimize its aerodynamic and aeroacoustic performances by considering the attached as well as the separated flow at the effective Reynolds number of , where the maximum lift was increased without sacrificing drag or overall noise at near- and poststall angles. Further aerodynamic and aeroacoustic tests indicated that a combination of LEU wavelength and amplitude gave an optimum LEU by considering the aerodynamic performance as well as the noise reduction. Particle image velocimetry measurements of the flow over the optimized airfoil showed biperiodic velocity fluctuations downstream of the LEU peaks that were associated with unsteady stall cell structure near the trailing edge.
Dominance of Gs in doubly Gs/Gi-coupled chimaeric A1/A2A adenosine receptors in HEK-293 cells (2000)

A(1) adenosine receptors inhibit adenylate cyclase by activating G(i)/G(o), whereas A(2A) receptors activate G(s). We examined how regions of A(1) and A(2A) receptors regulate coupling to G-proteins by constructing chimaeras in which the third intracellular loops (3ICL or L) and/or the C-termini (or T) were switched. Pertussis toxin (PTX) was used in membrane radioligand binding assays to calculate the fraction of recombinant receptors coupled to G(i)/G(o) and in whole cells to differentially influence agonist-stimulated cAMP accumulation. Switching A(1)/A(2A) 3ICL domains results in receptors that maintain binding selectivity for ligands but are doubly coupled. Receptor chimaeras with an A(1) 3ICL sequence (A(2A)/A(1)L or A(2A)/A(1)LT) respond to agonist stimulation with elevated cAMP despite being coupled predominantly to G(i)/G(o). These chimaeras have basal cAMP levels lower than those of wild-type A(2A) receptors, similar to wild-type A(1) receptors. The A(1) C-terminus modulates the coupling of receptors with A(1) 3ICL such that A(2A)/A(1)LT is better coupled to G(i)/G(o) than A(2A)/A(1)L. The C-terminus has little impact on coupling to receptors containing A(2A) 3ICL sequence. Our results show that the C-terminus sequence selectively facilitates coupling to G(i)/G(o) mediated by A(1) 3ICL and not by other intracellular domains that favour G(i) coupling. The C-terminus sequence has little or no effect on coupling to G(s). For doubly G(s)/G(i)-coupled adenosine receptors in HEK-293 cells, G(s)-mediated stimulation predominates over G(i)/G(o)-mediated inhibition of adenylate cyclase. We discuss the signalling consequences of simultaneously activating opposing G-proteins within single cells.
Analysis of Short-Term NBTI Effect on the Single-Event Upset Sensitivity of a 65nm SRAM using Two-Photon Absorption (2013)

International audience
Directed Evolution of an Feᴵᴵ-Dependent Halogenase for Asymmetric C(sp³)-H Chlorination (2019)

By using structure-guided directed evolution, the substrate scope of the FeII and a‑ketoglutarate dependent halogenase Wi‑WelO15 from Westiella intricata HT-29-1 was engineered to enable chemo-, regio- and diastereoselective chlorination of unactivated C(sp3)-H bonds using NaCl as chlorine source. While FeII dependent enzymes are often oxygen sensitive, variants of this halogenase could be screened in lysates under aerobic conditions. The new biocatalysts offer a sustainable approach for mild, late-stage chlorination on mg-scale of non-natural hapalindoles containing a ketone instead of an isonitrile functionality, thereby unlocking them for preparative biocatalysis.&lt;br&gt;
Investigating Engineering Students’ Mathematical Modeling Abilities In Capstone Design (2020)

Engineering capstone design is a culminating experience that is intended to provide an opportunity for students to apply their previous engineering knowledge to develop solutions to open-ended problems. Capstone design problems are often analytically complex, and their solutions integrate several disciplinary fundamentals, as well as more general design process knowledge. Often, the expectation is that a thorough or rigorous solution to a capstone level problem would include some type of computational or mathematical analysis appropriate to that discipline. However, engineering students often struggle in recognizing when and how disciplinary knowledge (e.g. mathematical analysis inherent in many engineering fundamentals) applies to their particular design solutions. This paper describes the strategy for and initial results of a study exploring how students use mathematical reasoning when developing design solutions. Specifically, we want to understand where students struggle in the development and implementation of a mathematical model. We conducted our study in a biomedical capstone (senior) design course. We presented students with a scenario based on a design problem in using phototherapy to treat jaundice, and asked specific questions relating to mathematical modeling in the solution to this problem.
Imaging performance of a full-ring prototype PET-MRI system based on four-layer DOI-PET detectors integrated with a RF coil (2015)

We are developing a PET system integrated with a birdcage RF-coil for PET-MRI in order to realize both high sensitivity and high spatial resolution of the PET image by using the 4-layered depth-of-interaction (DOI) PET detector. We constructed a full-ring prototype system and evaluated performances, especially imaging performance, of the prototype system in simultaneous measurement. The prototype system consists of eight four-layer DOI-PET detectors and a prototype birdcage RF-coil developed for the proposed system. The PET detectors consist of six monolithic multi-pixel photon counter array (S11064-050P), a readout circuit, fourlayer DOI scintillator arrays and a shielding box made of 35 μm thick copper foil. The crystal array consists of 2.0 mm x 2.0 mm x 5.0 mm LYSO crystals arranged in 38 x 6 x 4 layer. The RF-coil has eight coil elements and the eight PET detectors are positioned at each element gap. The diameter of the RF-coil elements is 261 mm. We conducted performance tests of the prototype system with a 3.0 T MRI (MAGNETOM Verio). Only the PET detectors, the RF-coil and the cables were in an MRI room during measurements. A data acquisition system and power supplies for the MPPCs and preamplifiers were outside the MRI room and connected to all the detectors through a penetration panel. As a result, the spatial resolutions of a Na-22 point source in the PET image were lower than 1.6 mm in whole the FOV due to the DOI capability. In addition, the influence of the simultaneous measurements on the PET performance is negligible. On the other hand, the SNR of the phantom image in the magnitude images was degraded from 259.7 to 209.4 due to noise contamination from the power supplies.
Simultaneous phase‐contrast MRI and PET for noninvasive quantification of cerebral blood flow and reactivity in healthy subjects and patients with cerebrovascular disease (2019)

3 Technical Efficacy Stage: 2 J. Magn. Reson. Imaging 2019. J. Magn. Reson. Imaging 2020;51:183-194.
Address generation for memories containing multiple arrays (2002)

This paper presents techniques for generating addresses for memories containing multiple arrays. Because these techniques rely on the inversion or rearrangement of address bits, they are faster and require less hardware to compute than offset addition. Use of these techniques can decrease effective access time to arrays and reduce address generation hardware. The primary drawback is that extra memory space is occasionally required by these techniques, but this extra memory space is on average only 4% and no worse than 25.2% of the utilized memory space. This amount of wasted address space is less than the amount required by similar techniques.
Meconium Aspiration Syndrome Induces Complement‐Associated Systemic Inflammatory Response in Newborn Piglets (2005)

The pathophysiology of meconium aspiration syndrome (MAS) is complex. We recently showed that meconium is a potent activator of complement. In the present study, we investigated whether the complement activation occurring in experimental MAS is associated with a systemic inflammatory response as judged by granulocyte activation and cytokine and chemokine release. MAS was induced by the instillation of meconium into the lungs of newborn piglets (n = 8). Control animals (n = 5) received saline under otherwise identical conditions. Haemodynamic and lung dynamic data were recorded. Complement activation, revealed by the terminal sC5b-9 complex (TCC), and cytokines [interleukin (IL)-6 and IL-8] were measured in plasma samples by enzyme immunoassays. The expression of CD18, CD11b and oxidative burst in granulocytes was measured in whole blood by flow cytometry. Plasma TCC increased rapidly in the MAS animals in contrast with controls (P < 0.0005). The TCC concentration correlated closely with oxygenation index (r = 0.48, P < 0.0005) and ventilation index (r = 0.57, P < 0.0005) and inversely with lung compliance (r = -0.63, P < 0.0005). IL-6 and IL-8 increased in MAS animals compared with the controls (P = 0.002 and P < 0.001, respectively). Granulocyte oxidative burst declined significantly in the MAS animals compared with the controls (P < 0.02). TCC correlated significantly with IL-6 (r = 0.64, P < 0.0005) and IL-8 (r = 0.32; P = 0.03) and inversely with oxidative burst (r = -0.37; P = 0.02). A systemic inflammatory response associated with complement activation is seen in experimental MAS. This reaction may contribute to the pathogenesis of MAS.
Learning to Solve the AC-OPF using Sensitivity-Informed Deep Neural Networks (2021)

To shift the computational burden from real-time to offline in delay-critical power systems applications, recent works entertain the idea of using a deep neural network (DNN) to predict the solutions of the AC optimal power flow (AC-OPF) once presented load demands. As network topologies may change, training this DNN in a sample-efficient manner becomes a necessity. To improve data efficiency, this work utilizes the fact OPF data are not simple training labels, but constitute the solutions of a parametric optimization problem. We thus advocate training a sensitivity-informed DNN (SI-DNN) to match not only the OPF optimizers, but also their partial derivatives with respect to the OPF parameters (loads). It is shown that the required Jacobian matrices do exist under mild conditions, and can be readily computed from the related primal/dual solutions. The proposed SI-DNN is compatible with a broad range of OPF solvers, including a non-convex quadratically constrained quadratic program (QCQP), its semidefinite program (SDP) relaxation, and MATPOWER; while SI-DNN can be seamlessly integrated in other learning-to-OPF schemes. Numerical tests on three benchmark power systems corroborate the advanced generalization and constraint satisfaction capabilities for the OPF solutions predicted by an SI-DNN over a conventionally trained DNN, especially in low-data setups.
Rapid Synthesis of a Lipocationic Polyester Library via Ring-Opening Polymerization of Functional Valerolactones for Efficacious siRNA Delivery (2015)

The ability to control chemical functionality is an exciting feature of modern polymer science that enables precise design of drug delivery systems. Ring-opening polymerization of functional monomers has emerged as a versatile method to prepare clinically translatable degradable polyesters.1 A variety of functional groups have been introduced into lactones; however, the direct polymerization of tertiary amine functionalized cyclic esters has remained elusive. We report a strategy that enabled the rapid synthesis of >130 lipocationic polyesters directly from functional monomers without protecting groups. These polymers are highly effective for siRNA delivery at low doses in vitro and in vivo.
Morphology of Electrophoretically Deposited Films on Electrode Strips (2012)

Studies of the kinetics of electrophoretic deposition (EPD) processes have generally focused on electrode geometries that yield analytical solutions, such as infinite parallel planes and concentric cylinders. In this article, we construct a finite element model for EPD of material onto a planar strip electrode, which shows excellent qualitative agreement to experimental results in a similar system. Notably, we demonstrate that the presence of the edges of the electrode lead to a singularity in the electric field that significantly affects the morphology of the deposit at short times or for thin deposits.
What are the mechanisms involved in the emergence of cooperativity?: The spider model (1997)

info:eu-repo/semantics/published
Towards a non-linear narrative construction (2003)

This article describes the implementation of a system that 'imagines' while a movie is being played by finding associations in the movie's content and presenting them to the viewer. This related information, in the form of images and movie clips, helps enhance the viewer's experience in a new immerse environment.
Preparation of small-sized graphene oxide sheets and their biological applications (2015)

By using carbon nanohorns as starting materials, small- and uniform-sized graphene oxide (S-GO) sheets can be prepared in high yields via an oxidation method. The obtained S-GO sheets have a band-like structure with a length of 20-50 nm, a width of 2-10 nm, and a thickness of 0.5-5 nm. S-GO sheets are hydrophilic due to abundant oxygenated groups on the surfaces and edges; hence, this nanomaterial is highly dispersive in aqueous solutions and some hydrophilic organic solvents. Additionally, like other S-GO samples, the S-GO sheets prepared here are strongly fluorescent over the visible light wavelength region. These characteristics underscore the high potential of S-GO sheets for nanomedical and diagnostic applications. In proof-of-concept experiments, the S-GO sheets were conjugated with an arginine-glycine-aspartic acid derivative for tumour-targeting drug delivery applications, and with an immunoglobulin G antibody for immunoassay applications.
Gas separation by adsorption: technological drivers and opportunities for improvement (2019)

Chemical and petrochemical companies are increasingly realizing that their sustainable development critically depends upon development of new innovative processes that use more efficiently materials and energy. As overall separation/purification processes account for 40–60% of capital and operating costs, their amelioration can significantly reduce costs, energy use and waste generation by increasing profits. Gas separation by adsorption technology is a well-established unit operation in chemical and petrochemical industries due to its efficiency for dealing with a large range of gas separations including impurity removal, gas purification, and separation in recycle streams. The technology is far from being mature and opportunities to expand its domain of applicability and improve its efficiency are high in a context where better understanding of physical phenomena and technological progress in materials and engineering research are integrated. Major contribution for innovations in gas separation by adsorption technology relates to the discovery of new adsorbents with better separation characteristics coupled to process development and its optimization using multi-objective and multi-domain numerical approaches. This short review identifies technological gaps and drivers for accelerating the development of industrially important gas separations by adsorption.