gebhart/patrepeval huggingface dataset:
High average power mid IR ZnGeP<inf>2</inf> OPO using a wavelength-dependent polarization rotator (2006)

We report a compact 5.5 W mid-IR source that simultaneously generates four wavelengths (3.5 mum, 4.3 mum, 4.6 mum and 5.5 mum) collinearly from a single ZnGeP <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> OPO pumped by Type-II KTP OPO via wavelength-dependent polarization rotation.
Effects of intake and forage level on site and extent of digestion of plant cell wall monomeric components by sheep. (1990)

The objective of this experiment was to study the effects of level of intake and proportion of forage in the diet on site and extent of digestion of plant cell wall monomeric components (phenolics, neutral sugars) by sheep. Four Suffolk wethers (65 kg) with ruminal and duodenal cannulas were fed diets containing 75% (75A) or 25% (25A) alfalfa hay at two levels of intake, 1,700 (high intake, HI) or 1,100 (low intake, LI) g DM/d in a 4 X 4 latin-square design with a 2 X 2 factorial arrangement of treatments. The remainder of the diet consisted primarily of ground corn. Wethers had lower (P less than .05) total tract NDF and ADF digestibilities when fed 75A or at HI. An intake X forage level interaction (P less than .05) was noted for non-core lignin ferulic acid (FA) total tract disappearance, with wethers at LI having the highest, 25A-HI having the lowest, and 75A-HI having an intermediate FA disappearance. Wethers fed at LI had higher (P less than .05) total tract disappearance of non-core lignin p-coumaric acid (PCA). Wethers fed 75A had greater (P less than .05) total tract disappearances of core lignin vanillic acid (VA) and vanillin (VAN). Total tract digestibilities of the neutral monosaccharides glucose (GLC), xylose (XYL), arabinose (ARA) and galactose (GAL) were greater (P less than .05) for LI vs HI treatments. Wethers consuming 75A had greater (P less than .05) total tract ARA and GAL digestibilities, whereas wethers fed 25A had greater (P less than .05) total tract digestibilities of GLC and mannose (MAN). Diet composition and level of intake appear to influence site and extent of digestion of cell wall monomeric components.
Purinergic signalling in the subretinal space: a role in the communication between the retina and the RPE (2007)

The retinal pigment epithelium (RPE) is separated from the photoreceptor outer segments by the subretinal space. While the actual volume of this space is minimal, the communication that occurs across this microenvironment is important to the visual process, and accumulating evidence suggests the purines ATP and adenosine contribute to this communication. P1 and P2 receptors are localized to membranes on both the photoreceptor outer segments and on the apical membrane of the RPE which border subretinal space. ATP is released across the apical membrane of the RPE into this space in response to various triggers including glutamate and chemical ischemia. This ATP is dephosphorylated into adenosine by a series of ectoenzymes on the RPE apical membrane. Regulation of release and ectoenzyme activity in response to light-sensitive signals can alter the balance of purines in subretinal space, and thus coordinate communication across subretinal space with the visual process.
Preparation and 31P Chemical Shifts of Some Quaternary Phosphonium Salts (1971)

This paper reports the synthesis, analytical data, melting points, and phosphorus chemical shifts of over seventy quaternary phosphonium salts. Most of these data have not been reported.
Defining the Optimal Response Criteria for the Crohn's Disease Activity Index for Induction Studies in Patients With Mildly to Moderately Active Crohn's Disease (2008)

Clinical trial efficiency for induction studies in patients with mildly to moderately active Crohn's disease can be improved by using either a decrease in CDAI by > or =70 points for the last two consecutive visits or a decrease in baseline CDAI by > or =100 points as the primary end point for the trial. These findings are valid for patients with ileocecal Crohn's disease not refractory to aminosalicylates, corticosteroids, immunomodulators, and biologics, and patients who do not have stricturing or penetrating complications. It is unclear if these CDAI response criteria would similarly increase study efficiency in trials that recruited patients with moderately to severely active disease, patients refractory to aminosalicylates, corticosteroids, immunomodulators, and biologics, and patients with stricturing or penetrating complications.
Adaptable hetero Diels-Alder networks for fast self-healing under mild conditions (2014)

A novel adaptable network based on the reversible hetero Diels-Alder reaction of a cyanodithioester and cyclopentadiene is presented. Reversible between 50-120 °C, the adjustable and self-healing features of the network are evidenced via temperature dependent rheology experiments and repetitive tensile tests whereas the network's chemical structure is explored by temperature dependent 1H MAS-NMR spectroscopy. © 2014 WILEY-VCH Verlag GmbH &amp;amp; Co. KGaA, Weinheim.
Magnetic Biotransport: Analysis and Applications (2010)

Magnetic particles are finding increasing use in bioapplications, especially as carrier particles to transport biomaterials such as proteins, enzymes, nucleic acids and whole cells etc. Magnetic particles can be prepared with biofunctional coatings to target and label a specific biomaterial, and they enable controlled manipulation of a labeled biomaterial using an external magnetic field. In this review, we discuss the use of magnetic nanoparticles as transport agents in various bioapplications. We provide an overview of the properties of magnetic nanoparticles and their functionalization for bioapplications. We discuss the basic physics and equations governing the transport of magnetic particles at the micro- and nanoscale. We present two different transport models: a classical Newtonian model for predicting the motion of individual particles, and a drift-diffusion model for predicting the behavior of a concentration of nanoparticles that takes into account Brownian motion. We review specific magnetic biotransport applications including bioseparation, drug delivery and magnetofection. We demonstrate the transport models via application to these processes.
High carrier lifetimes in epitaxial germanium–tin/Al(In)As heterostructures with variable tin compositions (2022)

Carrier lifetimes vs. Sn compositions of the Ge 1− y Sn y /III–V heterostructures.
All-Photonic in-Memory Computing Based on Phase-Change Materials (2019)

We experimentally demonstrate, for the first time, co-located data storage and processing (i.e. in-memory computing) on an integrated photonic platform based on nonvolatile phase-change materials. © 2019 The Author(s)
A deletion mutant of Pseudomonas exotoxin-A fused to recombinant human interleukin-9 (rhIL-9-ETA') shows specific cytotoxicity against IL-9-receptor-expressing cell lines. (1996)

The receptor for human interleukin-9 (hIL-9) might be a target for selective immunotherapy. It is expressed on a variety of malignant cells, including Hodgkin's lymphoma, non-Hodgkin lymphoma and acute myeloid leukemia (AML). We therefore constructed a new chimeric toxin by fusing hIL-9-cDNA to modified Pseudomonas aeruginosa exotoxin A (ETA'). The binding properties of the new recombinant protein, rhIL-9-ETA', were assessed on different cell lines expressing the hIL-9 receptor. The antitumor potency of rhIL-9-ETA' was evaluated against the Hodgkin-derived cell lines L540Cy, KM-H2 and L1236, the Burkitt lymphoma cell line Daudi, the erythroleukemia cell line K562, and the mastocytoma cell line P815-hIL9R, transfected with hIL-9 receptor cDNA. Recombinant hIL-9-ETA' exhibited potent specific cytotoxic effects against P815-hIL9R, K562 and L1236 cells, inhibiting protein synthesis by 50% (IC50) at concentrations of 0.05, 0.58 and 3 micrograms/ml respectively. The cytotoxic effect was abrogated after addition of polyclonal antibodies against the human IL-9. rhIL-9-ETA' might be of potential use against hIL-9R-expressing malignancies.
Mapping dominant-negative mutations of anthrax protective antigen by scanning mutagenesis (2003)

The protective antigen (PA) moiety of anthrax toxin transports edema factor and lethal factor to the cytosol of mammalian cells by a mechanism that depends on its ability to oligomerize and form pores in the endosomal membrane. Previously, some mutated forms of PA, designated dominant negative (DN), were found to coassemble with wild-type PA and generate defective heptameric pore-precursors (prepores). Prepores containing DN-PA are impaired in pore formation and in translocating edema factor and lethal factor across the endosomal membrane. To create a more comprehensive map of sites within PA where a single amino acid replacement can give a DN phenotype, we used automated systems to generate a Cys-replacement mutation for each of the 568 residues of PA63, the active 63-kDa proteolytic fragment of PA. Thirty-three mutations that reduced PA's ability to mediate toxicity at least 100-fold were identified in all four domains of PA63. A majority (22) were in domain 2, the pore-forming domain. Seven of the domain-2 mutations, located in or adjacent to the 2beta6 strand, the 2beta7 strand, and the 2beta10-2beta11 loop, gave the DN phenotype. This study demonstrates the feasibility of high-throughput scanning mutagenesis of a moderate sized protein. The results show that DN mutations cluster in a single domain and implicate 2beta6 and 2beta7 strands and the 2beta10-2beta11 loop in the conformational rearrangement of the prepore to the pore. They also add to the repertoire of mutations available for structure-function studies and for designing new antitoxic agents for treatment of anthrax.
A phase I dose-escalation trial of alpha-tocopheryloxyacetic acid and concurrent trastuzumab in patients with treatment refractory HER2+ metastatic breast cancer. (2020)

TPS1103 Background: Metastatic HER2+ breast cancer, while initially responsive to trastuzumab, pertuzumab, and TDM-1, eventually progresses. The FDA recently approved trastuzumab deruxtecan, showing benefit in progression free survival but not in overall survival to date. Thus, additional therapies are needed for patients who progress on these HER2 directed agents. In metastatic HER2+ breast cancer, HER2-specific Th1 immune responses and higher CD4+ Th1 and CD8+ TIL levels are associated with a survival benefit. As this Type 1 immunity occurs in a minority of patients, additional immune modulation is needed. Alpha-tocopheryloxyacetic acid (α-TEA) has been reported to augment Type 1 immunity through increasing activated effector memory CD4+ and CD8+ T cells and decreasing immune suppressive CD4+CD25+ regulatory T cells in the tumor microenvironment. When given concurrently with an anti-HER2 antibody (7.16.4) in a pre-clinical tumor model, α-TEA synergized with 7.16.4 to induce tumor regression. We hypothesize that α-TEA and trastuzumab combination therapy in metastatic HER2+ breast cancer will be well tolerated, induce a clinical response, and augment anti-tumor Th1 immunity. Methods: Trial Design: Phase I dose escalation trial of α-TEA in combination with trastuzumab. Patients with metastatic HER2+ breast cancer will receive one of four doses sequentially of α-TEA: 0.6 mg/kg, 1.2 mg/kg, 2.4 mg/kg, and 4.8 mg/kg. Toxicity is assessed at baseline and through end of study. Blood and tumor tissue will be collected for immunologic monitoring and evaluation. Clinical response will be evaluated according to RECIST 1.1. Eligibility : Patients with progressive metastatic HER2+ breast cancer who have previously progressed on trastuzumab/pertuzumab and TDM-1. Specific Aims: Determine: (1) safety of four escalating doses of α-TEA with concurrent trastuzumab, (2) clinical response rate of α-TEA with concurrent trastuzumab (3) if concurrent α-TEA and trastuzumab increases activated effector memory CD4+ and CD8+ T cells, and (4) if concurrent α-TEA and trastuzumab increase the number of HER2-specific T cells. Statistical Methods: (1) The sample size of 24 and cohort size of 6 are determined by simulation experiments and practical consideration, (2) clinical response will be evaluated; overall PFS and OS will be calculated, (3) activated effector memory CD4+ and CD8+ T-cells will be analyzed (4) HER2-specific IFN-g/IL-10 ratios will be evaluated. Targeted Accrual : Twenty-four (24) patients. Clinical trial information: NCT04120246 .
Oxidative Stress Activates Membrane Ion Channels in Human Biliary Epithelial Cancer Cells (Mz-Cha-1). (2015)

Oxidative stress is known to contribute to cell damage. In several cell types, one of the earliest effects of oxidative stress is a rapid and substantial decrease in cell volume, which in turn regulates a broad range of cell functions, including development of apoptosis. Since volume regulation is closely coupled to membrane ion permeability, activation of ion channels may play an important role in oxidative stress-related cell injury. Oxidative stress plays a major role in a variety of liver diseases and bile duct epithelia cells (BDE) represent an important site of injury. We, therefore, investigated the functional interactions of oxidative stress, cell volume and ion permeability in a BDE model. Whole-cell patch clamp studies were performed in the human Mz-Cha 1 cell line. Oxidative stress was produced by addition of H₂O₂ to the bath solution. Changes of intracellular Ca(2+) concentration and of crosssectional area (for calculating cell volume) were monitored by laser scanning microscopy. Exposure of Mz-Cha 1 cells to H₂O₂ resulted in cell shrinkage and increase of the intracellular Ca(2+) concentration. Patch-clamp studies revealed that exposure to H₂O₂ also resulted in the activation of ion currents with a threshold of 10(-6) M H₂O₂. Ion substitution studies and blocker experiments identified the currents as representing an increase in membrane K(+) and Cl-permeability. Interestingly both ion channel activation and cell shrinkage had a close relationship to the applied H₂O₂ concentration and were significantly inhibited by intracellular Ca(2+) chelation. These data imply that in a BDE model, oxidative stress leads to cell shrinkage through activation of Ca(2+)-dependent K(+) and Cl(-) currents. Since cell shrinkage has been associated with increased cell damage, the opening of these ion channels might contribute to the high susceptibility of biliary epithelial cancer cells to oxidative stress.
Salvage laryngectomy and pharyngocutaneous fistulae after primary radiotherapy for head and neck cancer: A national survey from DAHANCA (2003)

The risk of fistulae is especially high in patients initially treated with radiotherapy for nonglottic advanced stage tumors. A significant decrease in the number of performed salvage laryngectomies over the 10 years was seen. Over the same time period, the annual number of fistulae remained almost constant. The resulting more than doubling of fistulae rate could thus in part be explained by less surgical routine.
Fault locating in ungrounded and high-resistance grounded systems (2001)

One of the most common and difficult problems to solve in industrial power systems is the location and elimination of the ground fault. Ground faults that occur in ungrounded and high-resistance grounded systems do not draw enough current to trigger circuit breaker or fuse operation, making them difficult to localize. Techniques currently used to track down faults are time consuming and cumbersome. A new approach developed for ground-fault localization on ungrounded and high-resistance grounded low-voltage systems is described. The system consists of a novel ground-fault relay that operates in conjunction with low-cost fault indicators permanently mounted in the circuit. The ground-fault relay employs digital signal processing techniques to detect the fault, identify the faulted phase, and measure the electrical distance away from the substation. The remote fault indicators are used to visually indicate where the fault is located. The resulting system provides a fast, easy, economical, and safe detection system for ground-fault localization.
Klebsiella pneumoniae Brain Abscess in Neonates: A Report of 2 Cases (2009)

Brain abscesses are uncommon in neonates. Klebsiella pneumoniae is a very uncommon microbial agent to cause brain abscess. We report 2 infants with Klebsiella pneumoniae sepsis who developed brain abscesses. One infant was a premature neonate who required mechanical ventilation for respiratory distress syndrome and subsequently developed nosocomial sepsis and brain abscess without evidence of preceding meningitis. Another infant was a full-term neonate without risk factors for sepsis who developed seizures on the sixth postnatal day and was found to have meningitis and brain abscess. Both infants had Klebsiella pneumoniae septicemia with multiple relatively large brain abscesses that responded poorly to antimicrobial agents. These infants were managed with transfontanel drainage and prolonged courses of antimicrobial agents. Key message of this report is that Klebsiella pneumoniae brain abscess may occur in the absence of meningitis and even in the absence of any identifiable risk factors.
Non-invasive characterization of the adipogenic differentiation of human bone marrow-derived mesenchymal stromal cells by HS-SPME/GC-MS (2014)

A non-invasive method to characterize human mesenchymal stromal cells during adipogenic differentiation was developed for the first time. Seven fatty acid methyl esters (FAMEs), including methyl laurate, methyl myristate, methyl palmitate, methyl linoleate, methyl oleate, methyl elaidate and methyl stearate, were used for characterizing adipogenic differentiation using headspace solid-phase microextraction (HS-SPME) which is a very simple and non-invasive method for the extraction of volatile compounds. Glassware was used for culturing mesenchymal stromal cells rather than the common plasticware to minimize contamination by volatile impurities. The optimal SPME fiber was selected by comparing diverse fibers containing two pure liquid polymers (PDMS and PA) and two porous solids (PDMS/DVB and CAR/PDMS). Using optimized procedures, we discovered that seven FAMEs were only detected in adipogenic differentiated mesenchymal stromal cells and not in the mesenchymal stromal cells before differentiation. These data could support the quality control of clinical mesenchymal stromal cell culture in the pharmaceutical industry in addition to the development of many clinical applications using mesenchymal stromal cells.
Personalized Pseudonyms for Servers in the Cloud (2017)

Abstract A considerable and growing fraction of servers, especially of web servers, is hosted in compute clouds. In this paper we opportunistically leverage this trend to improve privacy of clients from network attackers residing between the clients and the cloud: We design a system that can be deployed by the cloud operator to prevent a network adversary from determining which of the cloud’s tenant servers a client is accessing. The core innovation in our design is a PoPSiCl (pronounced “popsicle”), a persistent pseudonym for a tenant server that can be used by a single client to access the server, whose real identity is protected by the cloud from both passive and active network attackers. When instantiated for TLS-based access to web servers, our design works with all major browsers and requires no additional client-side software and minimal changes to the client user experience. Moreover, changes to tenant servers can be hidden in supporting software (operating systems and web-programming frameworks) without imposing on web-content development. Perhaps most notably, our system boosts privacy with minimal impact to web-browsing performance, after some initial setup during a user’s first access to each web server.
IEEE Journal on Exploratory Solid-State Computational Devices and Circuits Launches (2015)

Welcome to the inaugural issue of the IEEE Journal on Exploratory Solid-State Computational Devices and Circuits (JXCDC). This issue begins an era of having a multidisciplinary IEEE journal focused on publishing seminal research toward the future exploration of energy-efficient computing based on physics, materials, devices, circuits, and architecture that will be of great interest to researchers and those working in the IT industry.
Normal Free Interleukin-18 (IL-18) Plasma Levels in Dengue Virus Infection and the Need To Measure Both Total IL-18 and IL-18 Binding Protein Levels (2015)

Activated monocytes/macrophages and T lymphocytes that produce a cytokine storm are assumed to play a pivotal role in the pathogenesis of dengue. Interleukin-18 (IL-18) is a proinflammatory cytokine that is increased during dengue and known to induce gamma interferon (IFN-γ), which is crucial for dengue immune response. No data are available regarding the balance between IL-18 and its natural inhibitor IL-18 binding protein (IL-18BP) and how they interact within the inflammatory reaction of patients with dengue virus infections. Circulating levels of IL-18; IL-18BP; free, biologically active IL-18; the IL-18-dependent proinflammatory cytokine IFN-γ; monocyte-derived cytokines; and ferritin were assessed in adult Indonesian dengue patients (n = 95). Healthy individuals (n = 22) and leptospirosis (n = 19) and enteric fever (n = 6) patients served as controls. Total IL-18 levels were increased during dengue, leptospirosis, and enteric fever compared to healthy controls. However, due to a concurrent increase in IL-18BP levels, biologically active IL-18 levels remained similar in the different phases of dengue and in patients with leptospirosis. Biologically active IL-18 levels were also similar in patients with severe and nonsevere dengue. In conclusion, high total IL-18 and IL-18BP levels concur in dengue virus infections, leptospirosis, and enteric fever. This resulted in unchanged levels of free, biologically active IL-18 in dengue and leptospirosis, which underlines the importance of measuring both IL-18 and IL-18BP when studying the role of IL-18 in diseases.
A multiwavelength microflow cytometer. (2009)

Abstract : Fluorescence-based flow cytometry dates back to the 1960s. Essentially, cells or particles are aligned in a flow stream and optically interrogated. Size, density, and fluorescence at multiple wavelengths can be quantified. In many cases, tags, such as fluorescently labeled antibodies, are mixed with the samples prior to analysis so that specific targets or cell functions can be identified. Currently, large, complex, laboratory- based flow cytometers are required to perform medical diagnostics, such as white blood cell counts and immunoassays to detect infection, or for environmental monitoring applications, such as classification of marine algae. In the traditional design, the sample containing the particles or cells is pumped out of a small tube into a much larger, concentric pipe that is carrying filtered water. This hydrodynamic focusing puts all the particles into the center of the wider stream, which then is tapered to a smaller diameter. The particles are thus focused and pass single-file through the laser beams for analysis. Over the last decade, flow cytometers have become smaller in size and less expensive, but this sheath flow design is not amenable to miniaturization to the point that the systems are portable. NRL's Center for Bio/Molecular Science and Engineering has developed a microfluidic sheath flow system that is robust, simple to fabricate, and very compact. This sheath flow device forms the basis of a microflow cytometer that has demonstrated the capability for 4-color analysis that is competitive with the larger, commercial systems.