gebhart/patrepeval huggingface dataset:
The Reaction of Ferric Salts with Transferrin (1973)

The use of Fe3+ salts to saturate apotransferrin in buffer solutions and serum is often found in basic research and clinical procedures, despite a lack of adequate documentation. We have investigated the reaction of Fe3+ salts with apotransferrin using the absorption peak of Fe3+-transferrin at 470 nm to monitor the reaction. While titration of apo-transferrin with Fe3+-nitrilotriacetic acid gives a linear function and clear end point, titration with FeCl3 results in a sigmoidal-shaped curve and no clear end point is reached. The spectral data indicate that only 5 to 25% of the iron becomes bound when 1 eq of FeCl3 is added to apotransferrin at neutral pH. The remaining sites of the protein are vacant and available for reaction with Fe3+-nitrilotriacetic acid. Several methods for separating unbound FeCl3 from transferrin were attempted. They were, however, all unsuccessful and often gave misleading results. A stoichiometric reaction of Fe3+ salts with apotransferrin is only obtained when the reactants are initially mixed at a low pH and then carefully adjusted to neutrality. A method is also described for obtaining fully saturated, chelate-free Fe3+-transferrin, using Fe3+-nitrilotriacetic acid as the iron reagent. In view of the poor reactivity of Fe3+ salts with apotransferrin at neutral pH, proton release studies have been re-examined with Fe3+-nitrilotriacetic acid. A value close to 2.6 H+ released per Fe3+ bound was obtained by two methods.
Abstract 17784: The Genesips Project: an NHLBI-Sponsored induced Pluripotent Stem Cell (iPSC) Resource for the Study of Cardiovascular Diseases (2014)

The study of complex cardiovascular disease (CVD) has been hampered by the lack of appropriate human cellular model systems. In response, the NHBLI sponsored the NextGen Consortium, which encompasses 9 independent efforts spanning the portfolio of NHLBI related phenotypes. The goals of the consortium include: 1. To develop and improve methods for large-scale production and characterization of induced pluripotent stem cell (iPSC) models for CVD; 2. To create a resource of iPSC lines from a large number of phenotypically and genotypically characterized individuals. Our GENESiPS project is focused on insulin resistance (IR), a condition that affects 25-33% of the US population with serious health consequences including risk of type II diabetes and CVD. Although much is known about the physiological changes occurring during IR, little is known about the molecular pathways that drive the appearance of IR. Certain mature cell types as adipocytes, endothelial cells and skeletal muscle cells have been associated with the origin, maintenance and progression of IR. IPSCs offer an unprecedented opportunity of modeling human disease in vitro. We have created iPSC lines on insulin resistant and insulin sensitive patient groups with prior GWAS genotyping. Differentiation of these iPSCs to relevant cell types is providing the opportunity to correlate insulin sensitivity and high-density genetic variation data with specific cell-based profiling. We will validate our in vitro model and study the molecular pathways that define IR and its relationship to endothelial dysfunction. Relevant to the larger scientific community the establishment of iPSC lines on over 150 individuals (3 to 6 clones per patient) that reflect the range of insulin resistance in the general population. The iPSC lines were created from erythroblasts using the non-integrative Sendai virus system, passaged to allow clearance of Sendai virus and growth in feeder free conditions. The lines have been extensively characterized for markers of pluripotency (Tra1-60), sample identity and genomic integrity. Through the NextGen consortium, these lines, as well as phenotypic and genome-wide genotyping data will be available to qualified investigators.
Conception d'un capteur RFID sans puce à base de nanofils de silicium (2012)

National audience
(Invited) Opportunities and Challenges of Atom Switch for Low-Power Programmable Logic (2013)

Opportunities and challenges of atom switch featuring Cunanometer-scale conducting bridge in the solid-electrolyte arediscussed with comparing the other emerging memories. Atomswitch having the high on/off conductance ratio is suitable todirectly replace the conventional CMOS switch element, realizingthe low-power, nonvolatile programmable logic. Reliabilitychallenges of the atom switch are also discussed in the view pointof the stabilization of the on- and off-states.
An Opportunistic Unlicensed Spectrum Utilization Method for LTE and WiFi Coexistence System (2014)

In this paper, two novel mechanisms are developed for the coexistence of cellular and WiFi systems in unlicensed spectrum. In the opportunistic method, the small cell base station opportunistically selects traffic offloading or resource sharing on each WiFi access point (AP). In the hybrid method, the base station simultaneously offloads users and shares the unlicensed spectrum of each AP. The performances of the proposed methods are analyzed and compared. We find that traffic offloading can achieve better performance than resource sharing when the number of existing WiFi users is below a threshold and the hybrid method achieves the same performance as the resource sharing method when existing WiFi user number is large enough. Numerical results are presented to demonstrate the effectiveness of the proposed methods.
Sequential Best Integer-Equivariant Estimation for GNSS (2014)

The key to high precision parameter estimation in global navigation satellite system (GNSS) applications is to properly deal with the integer valued carrier-phase ambiguities. The class of integer estimators fixes all ambiguities to integer values. This can also decrease the precision of the estimates of the non-ambiguity parameters, if the fixing is incorrect. The best integer-equivariant (BIE) estimator is optimal in the sense of minimizing the mean-squared error (MSE) of both the ambiguities and the real valued parameters, regardless of the precision of the float solution. However, the BIE estimator comprises a search in the integer space of ambiguities, whose complexity grows exponentially with the number of ambiguities. This search is not feasible for large-scale network solutions. To overcome this problem, a sequential BIE (SBIE) algorithm is proposed, which shows close to optimal performance with only linearly increasing complexity. Numerical simulations are used to verify the performance of the SBIE algorithm. Copyright © 2014 Institute of Navigation.
Second harmonic generation of pseudo mode-locked multi ten milliwatt picosecond Ti:sapphire laser (2004)

We report the single-pass second harmonic generation (SHG) of the picosecond Ti:sapphire laser with a periodically poled lithium niobate (PPLN) waveguide. We demonstrate a conversion efficiency of 37% for the 9-mW fundamental input power at 820 nm. This laser source can provide three types of pulsed modes such as picosecond, nanosecond, and contiuous wave, by adjusting the power of the pumping source. We compare the conversion efficiency in each mode, and clearly show that SHG efficiency depends on the pulsewidth, that is, the peak power of the laser source. As the temperature of the PPLN rises, the fundamental wavelength for phase-matching becomes longer. We indicate that the rate is about 0.06 nm/K.
Six-Coordinate Trigonal-Prismatic Complexes of First-Row Transition Metals1 (1966)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSix-Coordinate Trigonal-Prismatic Complexes of First-Row Transition Metals1Richard Eisenberg, Edward I. Stiefel, Robert C. Rosenberg, and Harry B. GrayCite this: J. Am. Chem. Soc. 1966, 88, 12, 2874–2876Publication Date (Print):June 1, 1966Publication History Published online1 May 2002Published inissue 1 June 1966https://pubs.acs.org/doi/10.1021/ja00964a061https://doi.org/10.1021/ja00964a061research-articleACS PublicationsRequest reuse permissionsArticle Views271Altmetric-Citations51LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
(Invited) The New Paradigm in Passive Sweat Wearables: Temporal Profiling of Biomarkers to Elucidate the Relationship between Stress and Inflammation in Passively Expressed Eccrine Sweat (2021)

When the body is under stress, a lot of physiological processes work towards bringing the body back to its normal state. Cortisol, a stress hormone and TNF-alpha, a protein related to inflammation, are direct products of these physiological processes. The goal of this work is the demonstration of a biosensor that is capable of tracking these molecules in human sweat. The significance lies in simplifying chronic disease diagnostics, i.e. technique used to detect and monitor diseases in a non-invasive manner. This system is designed to be a wearable device that will track those physiologica process and use cortisol and TNF-alpha levels as indicators of user’s health status. Background: The HPA (Hypothalamic-Pituitary-Adrenal) axis is involved in maintaining homeostasis by engaging with the parasympathetic nervous system. During the process of chronic disease affliction to the body, this relationship is disturbed and there is an imbalance driven response observed with creation of disease related symptoms. This is of prime clinical importance when the body is chronically exposed to high levels of stress. By monitoring the two key components, cortisol, a direct product of the HPA axis and TNF-alpha, a pro-inflammatory cytokine, the manifestations of chronic stress on the homeostasis and diseased state of the body can be evaluated in a holistic/comprehensive manner. Materials and Methods : This work describes a novel sweat based sensing system which uses electrochemical modality to perform detection of cortisol and TNF-alpha. Results and Conclusion: This work highlights the development of an electrochemical detection system for the two biomarkers through human sweat. Limit of detection of the system is 1 ng/ml for cortisol and 1 pg/ml for TNF-alpha and dynamic range is 1-200ng/ml and 1-1000 pg/ml for cortisol and TNF-alpha respectively. This wearable system is designed to be a point of use, patient centric, chronic disease self-monitoring and management platform.
Outdoor experiment on user mobility using distributed MIMO beamforming for 5G radio access (2018)

This paper presents outdoor experimental results with a 4 layer single-user (SU)-multiple-input multiple-output (MIMO) 5G cellular radio access system. Beam tracking with narrow beams are evaluated in the 15-GHz frequency band in order to investigate the effects of user mobility. In addition, distributed MIMO beamforming functionality is implemented to extend coverage using multiple transmission points (TPs) supporting 2 layers each. The experimental result in an outdoor open space environment shows that the peak throughput is 12.1 Gbps on a public road at an average speed of approximately 10 km/h. The result also shows that more than 8 Gbps is achieved at an average speed of approximately 40 km/h. Furthermore, it is observed that throughput is remarkably degraded when the difference in the received power between the best and second best transmission point is larger than 15 dB and only 2 layer spatial multiplexing is supported from one of the transmission points.
IFM Receiver Test and Evaluation. (1979)

The Instantaneous Frequency Receiver (IFM) is a lightweight, low cost receiver which has potential for use in Electronic Warfare Systems. It is also susceptible to erroneous frequency reporting when presented with simultaneous signals. This report summarizes work done with the IFM receiver to statistically characterize the simultaneous signal problem areas and proposes possible methods of mitigating these problems. (Author)
Hybrid Type HTS-SQUID Magnetometer With Vibrating and Rotating Sample (2016)

Highly sensitive measurement of the very weak magnetic characteristics of magnetic particles in solution or of a solution itself is required for applications, such as biomedical examinations. In this paper, higher sensitivity and additional performance with relaxation measurement and harmonic signal detection were achieved by an improvement of the driving mechanism and the high-resolution electric magnet, and optimization of the superconducting quantum interference device (SQUID) detection unit. A servomotor was used for sample vibration and rotation with precise control and high speed. For detection of the M-H characteristics, the sample was vibrated under the magnetic field in the electromagnet. For measuring the relaxation of the magnetization, the sample was rotated. A first-order differential pickup coil with a normal conducting wire connected to the input coil of a high-temperature superconductor-SQUID was used to detect the magnetic signal from the sample. High-resolution measurement of the magnetic moment on the order of 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-11</sup> A m <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> was achieved with this system configuration. An ac magnetic field with a dc bias could then be applied to the sample for detection of the harmonic signal. The magnetic relaxation signal from the sample was measured by another of the same type of differential detection coil equipped outside of the electric magnet by rotating, instead of vibrating, the sample. The magnetization curve for a low concentration of iron nanoparticles with a superparamagnetic character in the solution was successfully measured using the developed magnetometer, and the relaxation phenomenon was also characterized.
Harnessing protein sensing ability of electrochemical biosensors via a controlled peptide receptor–electrode interface (2023)

In summary, screening and rational design of high-affinity peptides specific to cathepsin B for developing peptide-based electrochemical biosensors is reported for the first time. This study could promote the development of alternative antibody-free detection methods for clinical assays to test inflammatory bowel disease and other diseases.
Design and testing of silicon carbide JFETs based inverter (2009)

This paper presents the design and testing of a high frequency, high efficiency inverter using silicon carbide (SiC) JFETs power module. A rugged negative voltage gate drive circuit is used to solve the normally on problem of JFETs devices and avoid the bridge shot-through during power on or power off. The circuit can provide over-voltage protection, over current protection and over temperature protection circuits to ensure the safe operation of the SiC JFETs module and the integration of inverter system. SiC JFETs pspice model is established and discussed that the drive resistor voltage drop of SiC JFETs due to dv/dt is more severe than Si IGBT device compared the parameters of SiC JFET and Si IGBT at the same rated level. The simulation and measurement results show that SiC JFETs have short turn-on and turn-off times, which will result in lower switching losses than silicon (Si) IGBTs. The low on-resistance in SiC JFETs will result in lower conduction losses. The experiment results of a 1kW SiC JFETs based inverter showed at least 3% efficiency improvement by a SiC JFETs based inverter over a Si IGBT- based inverter.
Advanced Photonic Sensors and Applications II (2001)

Abstract : The proceeding volume contains oral and poster contributions from a symposium on Advanced Photonic Sensors and Applications at the SPIE-The International Society for Optical Engineering Nanyang Technological University, Singapore from 30 November to 3 December 1999. The volume comprises the area of electrooptical materials and photonic sensors design and fabrication. The symposium also focuses on advances in biomedical and biochemical sensors technology. Studies in microoptical metrology and vibration sensors are greatly highlighted and in detail discussed.
Microwave-assisted reaction of gelatin with tannic acid: non-thermal effect on the crosslinking process (2018)

The reaction of gelatin with tannic acid using microwave heating with simultaneous cooling (MHSC) in comparison with water bath heating was investigated to get some hints on the non-thermal effect of microwave. MHSC showed a better ability to accelerate the reaction rate. It also increased the turbidity, crosslinking degree, and viscosity of the crosslinked gelatin by 91 FTU, 6.8%, and 0.08 respectively, indicating that microwave could improve the properties of the product. Moreover, sodium dodecyl sulfate polyacrylamide gel electrophoresis patterns reflected that the crosslinked gelatin (MHSC) had a higher molecular weight as the gray levels decreased by 9.2% (235–245 kDa), 2.3% (135–145 kDa), and 9.3% (122–132 kDa). More homogeneous aggregations were identified at the crosslinked gelatin by atomic force microscopy. These results proved that microwave has a non-thermal effect in the gelatin crosslinking process, accelerating the crosslinking reaction, and improving the performance of the gelatin product, which may have a good use in industry.
RNA Separation: Substrates, Functional Groups, Mechanisms, and Control (2009)

This chapter contains sections titled: Solid-Phase Interaction The Solid-Phase Substrate and Attachment of Functional Groups Reverse-Phase Ion-Pairing Separation Mechanism Ion-Exchange Separation Mechanism Chaotropic Denaturing Interaction Mechanism Hybridization Gel Filtration References
Reaction Pathway and Free Energy Profile for Conversion of π-Conjugation Modes in Porphyrin Isomer (2012)

Porphycene is a structural isomer of porphyrin with 18π-conjugated aromatic character. Porphycene modified with trifluoromethyl (CF(3)) groups in the periphery of the framework readily affords the isolable 20π-conjugated antiaromatic form through a reaction with a proton-donating reductant. The 20π-conjugated form can be characterized by not only a variety of spectroscopies in solutions but also X-ray crystallography. This paper focuses on the free energy profile in the conversion of the 18π-conjugated porphycene into the 20π-conjugated form. From the results of kinetics, electrochemical measurements, and acid/base titrations, the 20π-conjugated CF(3) porphycene is formed by a concerted proton-electron transfer (CPET) from a hydroquinone reagent to the 18π-conjugated form. The hydrogen-atom affinity of the 18π-conjugated CF(3) porphycene (for two hydrogen atoms) was calculated to be -490 kJ mol(-1), indicating that the N-H bonds in the 20π-conjugated form are rather easily cleaved. This reflects the antiaromatic characteristics of the 20π-conjugated porphycene. We propose that the kinetic and thermochemical analysis using redox potentials and pK(a) data is applicable for determining the reaction pathway in conversion of aromatic/antiaromatic mode of π-conjugated macrocycles as well as popular investigations for oxidations of organic molecules.
A fast algorithm for calculating the dual Gabor window with integer oversampling (2004)

We consider Gabor expansion of signals that are oversampled in the time-frequency domain by an integer factor. We demonstrate that using a combination of nonminimum norm dual windows and discrete Fourier transforms (DFT), we can obtain the Gabor expansion very efficiently. The conventional method used to solve this problem is compared with our method and the advantages of our method are explicitly shown.
Electrical property of printed transistors fabricated with various types of carbon nanotube ink (2012)

Printed electronics is expected to be a low-cost, eco-friendly, and on-demand fabrication technology because it decreases the number of process steps and the amount of waste materials. Carbon nanotube (CNT) transistor arrays were fabricated on plastic films by maskless printing without any additional pattering process, and minimum materials were used. During fabrication, the morphology of the CNT random network was controlled by an adsorption mechanism on the surface to be printed, which resulted in excellent and uniform electrical properties. Based on the fabrication technology, various types of CNT ink were examined. A guiding principle for developing CNT ink was shown to improve the TFT performance.
Non‐depleting anti‐CD4, but not anti‐CD8, antibody induces long‐term survival of xenogeneic and allogeneic hearts in α1,3‐galactosyltransferase knockout (GT‐Ko) mice (2000)

Abstract: The anti‐galactose‐α1,3‐galactose (Gal) antibody (Ab) response following pig‐to‐human transplantation is vigorous and largely resistant to currently available immunosuppression. The recent generation of GT‐Ko mice provides a unique opportunity to study the immunological basis of xenograft‐elicited anti‐Gal Ab response in vivo, and to test the efficacy of various strategies at controlling this Ab response [ 1 ]. In this study, we compared the ability of non‐depleting anti‐CD4 and anti‐CD8 to control rejection and antibody production in GT‐Ko mice following xenograft and allograft transplantation. Hearts from baby Lewis rat or C3H mice were transplanted heterotopically into GT‐Ko. Non‐depleting anti‐CD4 (YTS177) and anti‐CD8 (YTS105) Abs were used at 1 mg/mouse, and given as four doses daily from day −2 to 1 then q.o.d. till day 21. Xenograft rejection occurred at 3 to 5 days post‐transplantation in untreated GT‐Ko recipients, and was histologically characterized as vascular rejection. Anti‐CD4, but not anti‐CD8, Ab treatment prolonged xenograft survival to 68 to 74 days and inhibited anti‐Gal Ab as well as xeno‐Ab production. In four of the five hearts from anti‐CD4 mAbs‐treated GT‐Ko mice, we observed classic signs of chronic rejection, namely, thickened intima in the lumen of vessels, significant IgM deposition, fibrosis and modest mononuclear cell infiltrate of Mac‐1 + macrophages and scattered T cells (CD8 &gt; CD4). Xenograft rejection in untreated, as well as anti‐CD4‐ and anti‐CD8‐treated, recipients was associated with increased intragraft IL‐6, IFN‐γ and IL‐10 mRNA. C3H allografts were rejected in 7 to 9 days by untreated GT‐Ko mice and were histologically characterized as cellular rejection. Treatment with anti‐CD4 and anti‐CD8 mAb resulted in graft survivals of &gt; 94.8 and 11.8 days, respectively. Anti‐CD4 mAb treatment resulted in a transient inhibition of alloreactive and anti‐Gal Ab production. The presence of circulating alloreactive and anti‐Gal Abs at &gt; 50 days post‐transplant was associated with significant IgM and IgG deposition in the graft. Yet, in the anti‐CD4 mAb‐treated group, the allografts showed no signs of rejection at the time of sacrifice (&gt; 100 days post‐transplantation). All rejected allografts had elevated levels of intragraft IL‐6, IFN‐γ and IL‐10 mRNA, while the long‐surviving anti‐CD4‐treated allografts had reduced mRNA levels of these cytokines. Collectively, our studies suggest that the elicited xeno‐antibody production and anti‐Gal Ab production in GT‐Ko mice are CD4 + T‐cell dependent. The majority of xenografts succumbed to chronic rejection, while allografts survived with minimal histological change, despite elevated levels of circulating alloAbs. Thus, immunosuppression with anti‐CD4 mAb therapy induces long‐term survival of allografts more effectively than to xenografts.