gebhart/patrepeval huggingface dataset:
In Vivo Assessment of Bone Regeneration in Alginate/Bone ECM Hydrogels with Incorporated Skeletal Stem Cells and Single Growth Factors (2015)

The current study has investigated the use of decellularised, demineralised bone extracellular matrix (ECM) hydrogel constructs for in vivo tissue mineralisation and bone formation. Stro-1-enriched human bone marrow stromal cells were incorporated together with select growth factors including VEGF, TGF-β3, BMP-2, PTHrP and VitD3, to augment bone formation, and mixed with alginate for structural support. Growth factors were delivered through fast (non-osteogenic factors) and slow (osteogenic factors) release PLGA microparticles. Constructs of 5 mm length were implanted in vivo for 28 days within mice. Dense tissue assessed by micro-CT correlated with histologically assessed mineralised bone formation in all constructs. Exogenous growth factor addition did not enhance bone formation further compared to alginate/bone ECM (ALG/ECM) hydrogels alone. UV irradiation reduced bone formation through degradation of intrinsic growth factors within the bone ECM component and possibly also ECM cross-linking. BMP-2 and VitD3 rescued osteogenic induction. ALG/ECM hydrogels appeared highly osteoinductive and delivery of angiogenic or chondrogenic growth factors led to altered bone formation. All constructs demonstrated extensive host tissue invasion and vascularisation aiding integration and implant longevity. The proposed hydrogel system functioned without the need for growth factor incorporation or an exogenous inducible cell source. Optimal growth factor concentrations and spatiotemporal release profiles require further assessment, as the bone ECM component may suffer batch variability between donor materials. In summary, ALG/ECM hydrogels provide a versatile biomaterial scaffold for utilisation within regenerative medicine which may be tailored, ultimately, to form the tissue of choice through incorporation of select growth factors.
A Real Time Storm Surge Forecasting System Using ADCIRC (2008)

An automated storm surge forecasting system was created around ADCIRC to predict the storm surge from tropical cyclones in real time as a storm approaches. This system was then applied to Lake Pontchartrain in Southern Louisiana as a case study in order to assist the US Army Corps of Engineers with planning decisions that must be made as storms approach and make landfall. Surge forecasts are generated following each tropical storm advisory update issued by the National Hurricane Center. The general procedure is to create an ensemble of five ADCIRC storm surge runs based on the consensus storm forecast from the National Hurricane Center and perturbations to this forecast. Winds and pressure fields are generated using a parametric wind model (based on the Holland wind model) that has been coded as an ADCIRC subroutine to maximize execution speed. Initial outputs are water level and wind speed time series plots along the southern shore of Lake Pontchartrain near critical infrastructure. Results will be presented based on the forecasts for historical storms, as well as a summary of the system's performance on the case study site during the 2007 hurricane season.
Challenges in the Development of Intravenous Neurokinin‐1 Receptor Antagonists: Results of a Safety and Pharmacokinetics Dose‐Finding, Phase 1 Study of Intravenous Fosnetupitant (2022)

Oral NEPA is the fixed-combination antiemetic comprising netupitant (neurokinin-1 receptor antagonist [NK<sub>1</sub> RA]) and palonosetron (5-hydroxytryptamine-3 receptor antagonist [5-HT<sub>3</sub> RA]). Intravenous (IV) NEPA, containing fosnetupitant, a water-soluble N-phosphoryloxymethyl prodrug of netupitant, has been developed. Fosnetupitant does not require excipients or solubility enhancers often used to increase IV NK<sub>1</sub> RA water solubility, preventing the occurrence of hypersensitivity and infusion-site reactions associated with these products. In this phase 1 study, subjects received a 30-minute placebo or fosnetupitant (17.6-353 mg) infusion and an oral NEPA or placebo capsule, with 2-sequence crossover treatment for fosnetupitant 118- to 353-mg dose cohorts. IV fosnetupitant safety and pharmacokinetics were evaluated, and its equivalence to an oral netupitant 300-mg dose was defined. Overall, 158 healthy volunteers were enrolled. All adverse events (AEs) were mild or moderate in intensity. Doppler-identified infusion-site asymptomatic thrombosis occurred in 5.4% (fosnetupitant) and 1.2% (oral NEPA) of subjects. The frequency or number of treatment-related AEs did not increase with ascending fosnetupitant doses. The most common treatment-related AEs were headache (fosnetupitant, 8.1%; oral NEPA, 12.7%) and constipation (fosnetupitant, 1.4%; oral NEPA, 7.5%). A fosnetupitant 235-mg dose was equivalent, in terms of netupitant exposure, to 300-mg oral netupitant. The safety profile of a single fosnetupitant 235-mg infusion was similar to that of single-dose oral NEPA.
Supplementary Figure S3 from Efficient Payload Delivery by a Bispecific Antibody–Drug Conjugate Targeting HER2 and CD63 (2023)

&lt;p&gt;Schematic overview of the mechanism of action of bsHER2xCD63his-ADC&lt;/p&gt;
Nonlinear, noniterative Bayesian tomographic image reconstruction (1999)

In this research, rather than developing a forward model to be inverted, we propose directly modeling the inverse operator. The goal is to develop a non-iterative Bayesian reconstruction method which requires computation comparable to conventional FBP methods, but achieves quality competitive with that of iterative Bayesian methods such as maximum a posteriori probability (MAP). The method we propose, which we call nonlinear back projection (NBP), forms a back projected image cross-section by applying nonlinear filters to the projected data. This method attempts to directly model a type of optimal inverse operator through off-line training of the non-linear filters using example training data of known image cross sections and noisy realizations of projections. The Radon domain filtering is two-dimensional, exploiting redundancy among adjacent angles' measurements. This direct approach to modeling the inverse operator has several potential advantages which make it interesting. First, the elimination of iterative estimation should save computation time relative to common Bayesian techniques. Secondly, some of the inherently nonlinear attributes of the forward process may be implicitly incorporated into the training of the nonlinear backprojection. Finally, training based on sample images and projections may more effectively incorporate greater complexity in the statistical behavior of images than the simple Markov random field models found in most Bayesian formulations.
The spatial focusing of a leaky time reversal chaotic cavity (2007)

Elastic waves propagating inside a solid chaotic cavity create a diffusive random field that contains both longitudinal and shear waves. In the current paper, we are interested in the field radiated in a fluid in contact with such cavity. The goal of this paper is to predict the spatial focusing properties that can be obtained in the fluid using a time-reversal piezoelectric transducer in contact with the cavity. We present a statistical approach that supposes a fully diffused wavefield inside the cavity with an equipartition of energy between longitudinal and shear waves. We show that the critical angles of transmission in the solid–fluid interface generate a cut-off of the spatial frequencies and then a degradation in the spatial focusing. This limitation can be overcome using a rough surface. A set of experiments conducted in the MHz range confirm the theoretical model.
Gibt es prognostische Parameter bei endomikroskopisch und diätetischer gesicherter Nahrungsmittelsensitivität (NMS)? Untersuchungen an Reizdarmpatienten mit mittels konfokaler Endomikroskopie (EM) gesicherter Nahrungsmittelintoleranz und deutlicher Besserung unter 3-monatiger Auslassdiät (2017)

Studien zeigen, dass die EM sehr gut zur Diagnostik einer nicht Zöliakie/IgE-negativen NMS bei Reizdarmsyndrom (IBS) Patienten geeignet ist. Die Diagnose der NMS wird nach Allergenexposition (AE) mit einer erfolgreichen Langzeit-Ausschlussdiät bestätigt*. Viele IBS Patienten könnten einfacher und früher diagnostiziert werden, wenn klinische, histologische und biochemische Parameter für die Differenzierung von IBS und möglicher NMS zur Verfügung stünden.
Partial agonists of S1P1: Exploring side chain modifications of a bicyclic series to modulate the PK, PD, and safety profiles (2020)

Sphingosine-1-phosphate (S1P) is the endogenous ligand for the sphingosine-1-phosphate G-protein coupled receptors (S1P1-5). The interaction of S1P with the S1P receptors plays a fundamental physiological role in many processes including vascular stabilization, heart development, cancer angiogenesis and lymphocyte homing. Agonism of S1P1 has been shown to block lymphocyte
Contrasting Transcriptional Responses of a Virulent and an Attenuated Strain of Mycobacterium tuberculosis Infecting Macrophages (2010)

Along with the recent identification of single nucleotide polymorphisms in H37Ra when compared to H37Rv, our demonstration of differential expression of PhoP-regulated and ESX-1 region-related genes during macrophage infection further highlights the significance of these genes in the attenuation of H37Ra.
In Vitro Antibacterial Activities of JNJ-Q2, a New Broad-Spectrum Fluoroquinolone (2010)

JNJ-Q2, a novel fluorinated 4-quinolone, was evaluated for its antibacterial potency by broth and agar microdilution MIC methods in studies focused on skin and respiratory tract pathogens, including strains exhibiting contemporary fluoroquinolone resistance phenotypes. Against a set of 118 recent clinical isolates of Streptococcus pneumoniae, including fluoroquinolone-resistant variants bearing multiple DNA topoisomerase target mutations, an MIC(90) value for JNJ-Q2 of 0.12 microg/ml was determined, indicating that it was 32-fold more potent than moxifloxacin. Against a collection of 345 recently collected methicillin-resistant Staphylococcus aureus (MRSA) isolates, including 256 ciprofloxacin-resistant strains, the JNJ-Q2 MIC(90) value was 0.25 microg/ml, similarly indicating that it was 32-fold more potent than moxifloxacin. The activities of JNJ-Q2 against Gram-negative pathogens were generally comparable to those of moxifloxacin. In further studies, JNJ-Q2 exhibited bactericidal activities at 2x and 4x MIC levels against clinical isolates of S. pneumoniae and MRSA with various fluoroquinolone susceptibilities, and its activities were enhanced over those of moxifloxacin. In these studies, the activity exhibited against strains bearing gyrA, parC, or gyrA plus parC mutations was indicative of the relatively balanced (equipotent) activity of JNJ-Q2 against the DNA topoisomerase target enzymes. Finally, determination of the relative rates or frequencies of the spontaneous development of resistance to JNJ-Q2 at 2x and 4x MICs in S. pneumoniae, MRSA, and Escherichia coli were indicative of a lower potential for resistance development than that for current fluoroquinolones. In conclusion, JNJ-Q2 exhibits a range of antibacterial activities in vitro that is supportive of its further evaluation as a potential new agent for the treatment of skin and respiratory tract infections.
Association of heterocellular HPFH, beta(+)-thalassaemia, and delta beta(0)-thalassaemia: haematological and molecular aspects. (1984)

An Italian family in which heterocellular hereditary persistence of fetal haemoglobin (HPFH) interacts with both beta(+)- and delta beta-thalassaemia is described. The index case was an 8 year old girl who was presumed to inherit both heterocellular HPFH and beta (+)-thalassaemia from her mother and delta beta-thalassaemia from her father. She was healthy and never needed blood transfusions. The possible contribution of heterocellular HPFH to the less severe expression of the compound delta beta/beta(+)-thalassaemia heterozygosity is discussed. By DNA analysis the specific delta beta-thalassaemia defect on the gamma delta beta globin gene region has been established. In addition, a previously unreported association of a polymorphic restriction site haplotype with a beta (+)-thalassaemia mutation has been observed.
Cell cycle progression dictates the requirement for BCL2 in natural killer cell survival (2017)

Natural killer (NK) cells are innate lymphoid cells with antitumor functions. Using an N-ethyl-N-nitrosourea (ENU)-induced mutagenesis screen in mice, we identified a strain with an NK cell deficiency caused by a hypomorphic mutation in the Bcl2 (B cell lymphoma 2) gene. Analysis of these mice and the conditional deletion of Bcl2 in NK cells revealed a nonredundant intrinsic requirement for BCL2 in NK cell survival. In these mice, NK cells in cycle were protected against apoptosis, and NK cell counts were restored in inflammatory conditions, suggesting a redundant role for BCL2 in proliferating NK cells. Consistent with this, cycling NK cells expressed higher MCL1 (myeloid cell leukemia 1) levels in both control and BCL2-null mice. Finally, we showed that deletion of BIM restored survival in BCL2-deficient but not MCL1-deficient NK cells. Overall, these data demonstrate an essential role for the binding of BCL2 to BIM in the survival of noncycling NK cells. They also favor a model in which MCL1 is the dominant survival protein in proliferating NK cells.
Nisoldipine as a vasodilator in endothelium-deprived large coronary arteries of isolated working rabbit hearts (1989)

The effect of nisoldipine on the vasoconstrictor effect of histamine on coronary vascular resistance, cardiac performance and myocardial oxygen consumption was determined in this study. In a supported working heart preparation a perfluorochemical emulsion (FC-43) was used a perfusate. To enhance the sensitivity of the large coronary arteries to vasoconstrictor stimuli, the endothelium was removed mechanically from the large coronary arteries. The changes in coronary artery diameter were documented by colour arteriography. Nisoldipine reduced the constriction of coronary arteries and the decrease in coronary flow induced by histamine. Nisoldipine abolished or reduced the decrease in cardiac output, left ventricular end systolic pressure, dP/dt and myocardial oxygen consumption. In the supported working heart preparation, nisoldipine is an effective dilator of large coronary arteries deprived of endothelial lining.
The mannose 6-phosphate receptor targeted with porphyrin-based periodic mesoporous organosilica nanoparticles for rhabdomyosarcoma theranostics (2020)

Porphyrin-based periodic mesoporous organosilica nanoparticles (PMO) synthesized from a large functional octatriethoxysilylated porphyrin precursor and allowing two-photon excitation photodynamic therapy (TPE-PDT) and NIR imaging were synthesized. These PMO were grafted with polyethylene glycol (PEG) moieties and an analogue of mannose 6-phosphate functionalized at the anomeric position (AMFA). AMFAs are known to efficiently target mannose 6-phosphate receptors (M6PRs) which are over-expressed in various cancers. Here, we demonstrated for the first time that M6PRs were over-expressed in rhabdomyosarcoma (RMS) cells and could be efficiently targeted with PMO-AMFA allowing TPE imaging and TPE-PDT of RMS cells. The comparison with healthy myoblasts demonstrated an absence of biological effects, suggesting a cancer cell specificity in the biomedical action observed.
AQX‐1125, small molecule SHIP1 activator inhibits bleomycin‐induced pulmonary fibrosis (2017)

AQX-1125 prevented bleomycin-induced lung injury during the inflammatory and fibrotic phases. AQX-1125, given therapeutically, modified disease progression and improved survival, as effectively as pirfenidone.
Fault Detection and Just‐in‐Time Maintenance in Adjustable‐Speed Drives (2012)

Abstract With growing electrification in transportation systems and widespread adoption of renewable energy systems, there is a growing need for the development of high‐efficiency motor/generator drives that can perform well under a wide range of operating conditions with a high degree of robustness and fault tolerance. In this article, different components of the drive have been explored and some of the potential causes of failure have been identified. Furthermore, some configurations that can be used for increasing fault tolerance of the drive have been presented and some common approaches used for this purpose have been discussed.
Krylov Subspace Iterations for the Calculation of K-Eigenvalues with sn Transport Codes (2002)

We apply the Implicitly Restarted Arnoldi Method (IRAM), a Krylov subspace iterative method, to the calculation of k-eigenvalues for criticality problems. We show that the method can be implemented with only modest changes to existing power iteration schemes in an SN transport code. Numerical results on three dimensional unstructured tetrahedral meshes are shown. Although we only compare the IRAM to unaccelerated power iteration, the results indicate that the IRAM is a potentially efficient and powerful technique, especially for problems with dominance ratios approaching unity. Key Words: criticality eigenvalues, Implicitly Restarted Arnoldi Method (IRAM), deterministic transport methods
Blind equalization of nonlinear channels from second order statistics using precoding and channel diversity (2002)

This paper considers the blind equalization problem for nonlinear Volterra type channels excited by real IID (independent and identically distributed) symbols. Previous work has shown that under the right conditions the equalizers can be found from the second order statistics of the channel output as long as the number of subchannels exceeds the number of kernels. In order to alleviate this requirement, we consider the use of a simple precoding device previous to transmission which provides a tradeoff between effective data rate and number of subchannels required. Necessary and sufficient conditions for blind equalizability under this scheme are given, and an algorithm for the computation of the equalizers is presented.
Optical properties of "diamondlike" carbon films: An ellipsometric study (1983)

The index of refraction and extinction coefficient are measured as a function of wavelength from 254 to 633 nm using reflection-null ellipsometry. It is shown that both rf glow-discharge-deposited and ion-beam-sputtered films have the same values of $n$ and $k$ over the measured wavelength range. Calculated values of absorption coefficient indicate that the absorption edge is not reached even at near ultraviolet wavelengths. Thermal cycling up to 500\ifmmode^\circ\else\textdegree\fi{}C does not change the optical properties significantly.
Notch3 Arg170Cys Knock-In Mice Display Pathologic and Clinical Features of the Neurovascular Disorder Cerebral Autosomal Dominant Arteriopathy With Subcortical Infarcts and Leukoencephalopathy (2011)

This model, for the first time, phenocopies the arteriopathy and the histopathologic as well as clinical features of CADASIL and may offer novel opportunities to investigate disease pathogenesis.
A unified synthetic strategy to introduce heteroatoms via electrochemical functionalization of alkyl organoboron reagents (2022)

Based on systematic electrochemical analysis, an integrated synthetic platform of C(sp3)-based organoboron com- pounds was established for the introduction of heteroatoms. The electrochemically mediated bond-forming strategy was shown to be highly effective for the functionalization of sp3-hybridized carbon atoms with significant steric hindrance. Moreover, virtually all the nonmetallic heteroatoms could be utilized as reaction partners using one unified protocol. The observed reactivity stems from the two consecutive single-electron oxidations of the substrate, which eventually generates an extremely reactive carbocation as the key intermediate. The detailed reaction profile could be elucidated through multifaceted electrochemical studies. Ultimately, a new di- mension in the activation strategies for organoboron compounds was accomplished through the electrochemically driven reaction development.
The Role of Glucagon, Catecholamines and Cortisol in Counterregulation of Insulin‐induced Hypoglycemia in Normal Man (1986)

To study the response of glucose counterregulation to insulin-induced hypoglycemia, six normals were given a 4-hour infusion of insulin (2.4 U/h) +/- somatostatin (50 micrograms/h). Supplementary glucagon (1.5 or 3.0 ng/kg/min) was given in additional experiments. In a separate study, glucagon was supplemented for 4 hours as a constant rate infusion (3.25 ng/kg/min) or at rates stepwise increasing from 1.5 to 5.0 ng/kg/min. Insulin decreased blood glucose by 1.5 mmol/l and simultaneous suppression of glucagon resulted in a more pronounced hypoglycemia enhancing the adrenaline and cortisol responses. The hyperglycemic effect of glucagon substitution (3 ng/kg/min) faded out after about 2 hours, whereafter exaggerated adrenaline and cortisol responses to hypoglycemia were seen. A comparison between the effects of steady state hyperglucagonemia and gradually appearing hyperglucagonemia on the counterregulation of hypoglycemia revealed no significant differences in glucose, adrenaline and cortisol responses to insulin. It is concluded that the glycemic effect of glucagon is transient in the hypoglycemic state. When the hepatic responsiveness to this hormone is decreased during hypoglycemia, adrenaline becomes the essential protective factor.
Blade Coating System for Organic Electronics (2019)

In this work we show the project and fabrication of a home-made blade coating system for deposition of solutions of organic semiconductor materials. The system was used for deposition of PEDOT:PSS, a common hole injection material in solution based organic light emitting diodes (OLEDs). Phosphorescent OLEDs were assembled with PEDOT:PSS deposited by blade coating and also by spin coating for comparison. Both devices presented similar performance with low leakage current and driving voltages around 6 V (at 20 mA/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> ). The efficiency for both devices was very similar (EQE ~ 17%, at 20 mA/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> ) showing that the PEDOT:PSS film obtained by blade coating has similar hole injection properties and performance as the film deposited by spin coating.
Protection against experimental Schistosoma mansoni schistosomiasis achieved by immunization with schistosomula released products antigens (SRP-A): role of IgE antibodies. (1986)

Schistosomula-released products (SRP-A) have been shown to induce preferentially a significant IgE response against Schistosoma mansoni schistosomula when injected into rats, in the absence of adjuvant. The present work provides additional evidence of the in vivo relevance of the anti-SRP-A target antigens. Two strains of rat (Brown Norway and Fischer) were immunized with SRP-A and infected percutaneously. A significant level of protection (up to 83% reduction in worm burden) was observed. Passive transfer experiments carried out with anti-SRP-A or IgE-depleted anti-SRP-A sera suggested the preponderant role of antibodies and particularly of IgE in the protective immunity developed by Fischer rats. Platelets and macrophages recovered from such immunized rats had surface IgE as demonstrated by immunofluorescence analysis with FITC anti-IgE, and have been shown to be directly cytotoxic for schistosomula. The chemiluminescence observed when the macrophages were incubated with anti-IgE suggested the presence of IgE on the surface of these cells.
In memorium: Mark Stockman (2021)

This Conference Presentation, “In memorium: Mark Stockman,” was recorded at SPIE Optics + Photonics 2021 held in San Diego, California, United States.