gebhart/patrepeval huggingface dataset:
Methoden zum Design digitaler Plattformen, Geschäftsmodelle und Service-Ökosysteme (2022)

Zusammenfassung Auf digitalen Märkten besteht, wer die zugrundeliegenden digitalen, unternehmensbezogenen oder akteursübergreifenden institutionellen Arrangements versteht und aktiv gestaltet. Dazu werden ausgereifte, von Forschung und Praxis diskutierte und gemeinsam weiter entwickelte Methoden zum Design digitaler Plattformen, Geschäftsmodelle und Service-Ökosysteme benötigt. Einen gemeinschaftlichen Aufschlag hierzu macht der folgende Artikel. Er entstand aus einem offenen Aufruf in der Wirtschaftsinformatik-Community – über Mailing-Listen und soziale Medien. Im Ergebnis führen sieben Forschergruppen (oder einzelne Forscher und Forscherinnen) ihre aktuellen Erkenntnisse zu diesem Thema zusammen.
Supplementary Data from Activated Platelets Provide a Functional Microenvironment for the Antiangiogenic Fragment of Histidine-Rich Glycoprotein (2023)

Supplementary Data from Activated Platelets Provide a Functional Microenvironment for the Antiangiogenic Fragment of Histidine-Rich Glycoprotein
Generating Oscillatory Behavior by Applying a Magnetic Field during Electrocatalytic Oxidation of Glycerol (2022)

This work demonstrates how the application of a magnetic field during electrocatalysis can affect the transport of reactants and products at the electrode surface and, under certain conditions, generate complex, oscillatory behavior in amperometric experiments. During the electrocatalytic oxidation of glycerol (EOG), the Lorentz force acts upon hydronium and hydroxide ionic currents to produce fluidic convection, which serves to enhance the mass transport of glycerol and glyceraldehyde. Particle imaging velocimetry shows that the convective fluid flow field depends nonlinearly on the viscosity of the solution (dictated by the concentration of glycerol, which ranged from 2.5% to 40% v/v, to give a viscosity range of 1.3–5.3 mPa s). The nonlinear dependence of velocity of the fluid flow on the viscosity of the electrolyte generates time-delayed negative feedback during EOG and results in chemical oscillations. This time-delayed feedback is due to two coupled steps: (i) oxidation of glycerol rapidly decreases the viscosity of the electrolyte near the anode and (ii) at low viscosities magnetic fields increase the rate of mass transport, which subsequently increases the viscosity of the electrolyte near the anode (by increasing the concentration of glycerol). These chemical oscillations can be used to enhance the selectivity of EOG to glyceric acid by a factor of 2.1. This work focuses on the effects of magnetic fields on EOG using a combination of fluid flow analysis, rotating-disk electrode experiments, and electrochemical simulations.
Blood mass spectrometry detects residual disease better than standard techniques in light-chain amyloidosis (2020)

In patients with immunoglobulin light-chain (AL) amyloidosis, depth of hematologic response correlates with both organ response and overall survival. Our group has demonstrated that screening with a matrix-assisted laser desorption/ionization-time-of-flight (TOF) mass spectrometry (MS) is a quick, sensitive, and accurate means to diagnose and monitor the serum of patients with plasma cell disorders. Microflow liquid chromatography coupled with electrospray ionization and quadrupole TOF MS adds further sensitivity. We identified 33 patients with AL amyloidosis who achieved amyloid complete hematologic response, who also had negative bone marrow by six-color flow cytometry, and who had paired serum samples to test by MS. These samples were subjected to blood MS. Four patients (12%) were found to have residual disease by these techniques. The presence of residual disease by MS was associated with a poorer time to progression (at 50 months 75% versus 13%, p = 0.003). MS of the blood out-performed serum and urine immunofixation, the serum immunoglobulin free light chain, and six-color flow cytometry of the bone marrow in detecting residual disease. Additional studies that include urine MS and next-generation techniques to detect clonal plasma cells in the bone marrow will further elucidate the full potential of this technique.
Corezilla: Build and Tame the Multicore Beast? (2007)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.
Ras Proteins: Recent Advances and New Functions (1999)

T HE RAS FAMILY comprises H-Ras, K-Ras 4A, K-Ras 4B, N-Ras, and other homologous proteins such as R-Ras, TC21, Rap, and Ral. Ras protein function is controlled by a guanosine triphosphate-guanosine diphosphate (GTP-GDP) cycle that is regulated by at least 2 distinct classes of regulatory proteins.[1
Photonic crystal devices formed by a charged-particle beam (2023)

A photonic crystal device and method. The photonic crystal device comprises a substrate with at least one photonic crystal formed thereon by a charged-particle beam deposition method. Each photonic crystal comprises a plurality of spaced elements having a composition different from the substrate, and may further include one or more impurity elements substituted for spaced elements. Embodiments of the present invention may be provided as electromagnetic wave filters, polarizers, resonators, sources, mirrors, beam directors and antennas for use at wavelengths in the range from about 0.2 to 200 microns or longer. Additionally, photonic crystal devices may be provided with one or more electromagnetic waveguides adjacent to a photonic crystal for forming integrated electromagnetic circuits for use at optical, infrared, or millimeter-wave frequencies.
Radionuclide Sensors for Water Monitoring (2005)

Radionuclide contamination in the soil and groundwater at U.S. Department of Energy (DOE) sites is a severe problem that requires monitoring and remediation. Radionuclide measurement techniques are needed to monitor surface waters, groundwater, and process waters. Typically, water samples are collected and transported to an analytical laboratory, where costly radiochemical analyses are performed. To date, there has been very little development of selective radionuclide sensors for alpha- and beta-emitting radionuclides such as 90Sr, 99Tc, and various actinides of interest.
A novel collector for imaging detector applications (2003)

In this preliminary study, the design of a novel collector for semiconductor imaging devices is presented The novelty of the collector relies on geometrical and physical parameters, which add to the collector enhanced "Frisch-grid" capabilities. As a result, the proposed technology provides an effective mode to shield the electron-collecting electrode from the charge induced on it from moving positive ions and trapped charge. Therefore, the performance parameters of an imaging detector, such as spatial and contrast resolution, can be improved significantly.
Electrosprayed core–shell microspheres for protein delivery (2010)

This communication describes a single-step electrospraying technique that generates core-shell microspheres (CSMs) with encapsulated protein as the core and an amphiphilic biodegradable polymer as the shell. The protein release profiles of the electrosprayed CSMs showed steady release kinetics over 3 weeks without a significant initial burst.
Rapid31P spectroscopy on a 4‐T whole‐body system (1988)

Initial 31P spectroscopy results from a 4-T whole-body system are reported. Localized spectra from the human head, liver, and calf were obtained using DRESS, slice-interleaved DRESS, and volume 3DFT spectroscopic imaging techniques. Substantial reductions in data acquisition times to 10 s-4 min were achieved relative to previous similar experiments at 1.5 T. Some gain in spectral resolution (as measured in ppm) was also realized in the head.
Nitric Oxide Promotes Proliferation and Plasminogen Activator Production by Coronary Venular Endothelium Through Endogenous bFGF (1997)

We reported previously that NO is responsible for the angiogenesis produced by endothelium-dependent vasodilating peptides. To investigate the mechanisms by which NO controls angiogenesis, NO was assessed for the ability to affect cell proliferation and upregulation of urokinase-type plasminogen activator (uPA) induced by basic fibroblast growth factor (bFGF) when added exogenously to or when produced endogenously by coronary venular endothelial cells (CVECs). The treatment of the cells with the NO donor sodium nitroprusside (NaNp) induced uPA upregulation and cell proliferation, which were prevented by anti-bFGF antibodies. Similarly, the NO-dependent mitogenic activity of the vasodilating peptide substance P (SP) was blocked by anti-bFGF antibodies, thus implicating endogenous bFGF in the NO-induced response. NaNp and SP induced bFGF expression as measured by Western blot analysis of CVEC extracts and by differential reverse transcriptase-polymerase chain reaction of bFGF mRNA. SP-induced upregulation of bFGF was prevented by the NO synthase inhibitor N omega-monomethyl-L-arginine. We conclude that NO promotes cell proliferation and uPA upregulation in CVECs by inducing endogenous bFGF and that this pathway mediates the angiogenetic response to the vasoactive neuropeptide SP. This signaling paradigm may provide an important link between shear rate, NO, bFGF, and coronary angiogenesis.
Micromachined Thermal Effect Array by Joule Heating for Bio Applications (2008)

We have developed a micro local heating device for bio applications using micro-electro-mechanical systems (MEMS) technology. The device has a two-dimensional microdish array, which contains a series of diodes. The microdish array is fabricated by means of a combination of complementary metal–oxide–semiconductor (CMOS) technology and MEMS post-processing. The microdish array has a miniature cavity for thermal separation from substrate. These microdish arrays can be generated by a large forward current based on Joule heating. In this paper, we describe the direct heating of the whole microdish array and just an arbitrary portion of the microdish consisting of the device by Joule heating. In the localized selective heating, the highest temperature, 62.6 °C, from all the dishes was confirmed. More than twice the heat effect was achieved in heating an arbitrary portion compared with heating the whole microdish.
Performance Assessment of Active Aerodynamic Surfaces for Comfort and Handling Optimization in Sport Cars (2015)

This paper investigates the use of active aerodynamic surfaces (AASs) to enhance the ride comfort of sport vehicles. AASs are constituted by airfoils that are properly placed on the vehicle chassis, and whose angle of attack can be modulated at high frequency (10 Hz). Thus, AASs can be used to generate a lift force that helps in controlling the vertical acceleration of the sprung mass without negatively affecting the unsprung mass. Thanks to this property, AAS systems can overcome the tradeoff between comfort and road holding. In this paper, a control system is presented along with a detailed analysis of several design choices (controller bandwidth, surface size, and actuator requirements). Furthermore, the effect of several environmental factors is considered: 1) vehicle mass; 2) vehicle velocity; and 3) road roughness. A specific analysis shows that the proposed system can be integrated with classical semiactive suspension control algorithms. The system is validated on a complete vehicle model, showing improvements of the order of 30% in ride comfort with no negative effects on road holding.
Abstract B51: The tyrphostin, NT157, suppresses insulin receptor substrates and augments therapeutic response of prostate cancer (2015)

Abstract Insulin-like growth factor (IGF) signaling is associated with castrate-resistant prostate cancer (CRPC) progression. Insulin receptor substrates 1 and 2 (IRS1/2) mediate mitogenic and anti-apoptotic signaling from IGF-1 receptor (IGF-1R), insulin receptor and other oncoproteins. This study demonstrates that IRS1/2 expression is increased in PCa and, persists in CRPC. Furthermore, this study assesses the anti-cancer activity of NT157, a small-molecule tyrphostin targeting IRS proteins, using androgen-responsive (LNCaP) and -independent (PC3) prostate cancer cells in vitro and in vivo. NT157 treatment resulted in dose-dependent inhibition of IGF1R activation, suppression of IRS protein expression, inhibition of IGF-1-induced AKT activation, but increased ERK activation in NT157-treated cells in vitro. These effects were correlated with decreased proliferation and increasing apoptosis of LNCaP cells and increasing G2M arrest in PC3 cells. NT157 also suppressed androgen-responsive growth, delayed CRPC progression of LNCaP xenografts and suppressed PC3 tumor growth alone and in combination with docetaxel. This study reports the first preclinical proof-of-principle data that this novel small-molecule tyrosine kinase inhibitor targeting human IRS suppresses IRS1/2 expression, delays CRPC progression, and suppresses growth of CRPC tumors in vitro and in vivo. Demonstration that IRS expression can be increased in response to a variety of stressors that may lead to resistance or reduced effect of the therapies indicate that NT157-mediated IRS1/2 down-regulation is a novel therapeutic approach for management of advanced prostate cancer. Citation Format: Naokazu Ibuki, Mazyar Ghaffari, Hadas Reuveni, Mitali Pandey, Ladan Fazli, Haruhito Azuma, Martin E. Gleave, Alexander Levitzki, Michael E. Cox. The tyrphostin, NT157, suppresses insulin receptor substrates and augments therapeutic response of prostate cancer. [abstract]. In: Proceedings of the AACR Special Conference: Targeting the PI3K-mTOR Network in Cancer; Sep 14-17, 2014; Philadelphia, PA. Philadelphia (PA): AACR; Mol Cancer Ther 2015;14(7 Suppl):Abstract nr B51.
Global gene expression profiling of pancreatic islets in mice during streptozotocin-induced β-cell damage and pancreatic Glp-1 gene therapy (2013)

Streptozotocin (STZ), a glucosamine-nitrosourea compound, has potent genotoxic effects on pancreatic β-cells and is frequently used to induce diabetes in experimental animals. Glucagon-like peptide-1 (GLP-1) has β-cell protective effects and is known to preserve β-cells from STZ treatment. In this study, we analyzed the mechanisms of STZ-induced diabetes and GLP-1-mediated β-cell protection in STZ-treated mice. At 1 week after multiple low-dose STZ administrations, pancreatic β-cells showed impaired insulin expression, while maintaining expression of nuclear Nkx6.1. This was accompanied by significant upregulation of p53-responsive genes in islets, including a mediator of cell cycle arrest, p21 (also known as Waf1 and Cip1). STZ treatment also suppressed expression of a wide range of genes linked with key β-cell functions or diabetes development, such as G6pc2, Slc2a2 (Glut2), Slc30a8, Neurod1, Ucn3, Gad1, Isl1, Foxa2, Vdr, Pdx1, Fkbp1b and Abcc8, suggesting global β-cell defects in STZ-treated islets. The Tmem229B, Prss53 and Ttc28 genes were highly expressed in untreated islets and strongly suppressed by STZ, suggesting their potential roles in β-cell function. When a pancreas-targeted adeno-associated virus (AAV) vector was employed for long-term Glp-1 gene delivery, pancreatic GLP-1 expression protected mice from STZ-induced diabetes through preservation of the β-cell mass. Despite its potent β-cell protective effects, however, pancreatic GLP-1 overexpression showed limited effects on the global gene expression profiles in the islets. Network analysis identified the programmed-cell-death-associated pathways as the most relevant network in Glp-1 gene therapy. Upon pancreatic GLP-1 expression, upregulation of Cxcl13 and Nptx2 was observed in STZ-damaged islets, but not in untreated normal islets. Given the pro-β-cell-survival effects of Cxcl12 (Sdf-1) in inducing GLP-1 production in α-cells, pancreatic GLP-1-mediated Cxcl13 induction might also play a crucial role in maintaining the integrity of β-cells in damaged islets.
Deconvolution of Molecular Weight Distributions Using Dynamic Flory‐Schulz Distributions (2004)

Abstract Summary: The deconvolution of molecular weight distributions (MWDs) may be useful for obtaining information about the polymerization kinetics and properties of catalytic systems. However, deconvolution techniques are normally based on steady‐state assumptions and very little has been reported about the use of non‐stationary approaches for the deconvolution of MWDs. In spite of this, polymerization reactions are often performed in batch or semi‐batch modes. For this reason, dynamic solutions are proposed here for simple kinetic models and are then used for deconvolution of actual MWD data. Deconvolution results obtained with dynamic models are compared to deconvolution results obtained with the standard stationary Flory‐Schulz distributions. For coordination polymerizations, results show that dynamic MWD models are able to describe experimental data with fewer catalytic sites, which indicates that the proper interpretation of the reaction dynamics may be of fundamental importance for kinetic characterization. On the other hand, reaction dynamics induced by modification of chain transfer agent concentration seem to play a minor role in the shape of the MWD in free‐radical polymerizations. This Figure illustrates that MWDs obtained at unsteady conditions should not be deconvoluted with standard steady‐state Flory‐Schulz distributions. image This Figure illustrates that MWDs obtained at unsteady conditions should not be deconvoluted with standard steady‐state Flory‐Schulz distributions.
Formation of Vinyl Halides via a Ruthenium-Catalyzed Three-Component Coupling (2002)

The ruthenium-catalyzed three-component coupling of an alkyne, an enone, and halide ion to form E- or Z-vinyl halides has been investigated. Through systematic optimization experiments, the conditions effecting the olefin selectivity were examined. In general, more polar solvents such as DMF favored the formation of the E-isomer, and less polar solvents such as acetone favored formation of the Z-isomer. The optimized conditions for the formation of E-vinyl chlorides were found to be the use of cyclopentadienyl ruthenium (II) cyclooctadiene chloride, stannic chloride pentahydrate as a cocatalyst, and for a chloride source, either ammonium chloride in DMF/water mixtures or tetramethylammonium chloride in DMF. A range of several other ruthenium (II) catalysts was also shown to be effective. A wide variety of vinyl chlorides could be formed under these conditions. Substrates with tethered alcohols or ketones either five or six carbons from the alkyne portion gave instead diketone or cyclohexenone products. For formation of vinyl bromides, a catalyst system involving the use of cyclopentadienylruthenium (II) tris(acetonitrile) hexafluorophosphate with stannic bromide as a cocatalyst was found to be most effective. The use of ammonium bromide in DMF/acetone mixtures was optimal for the synthesis of E-vinyl bromides, and the use of lithium bromide in acetone was optimal for formation of the corresponding Z-isomer. Under either set of conditions, a wide range of vinyl bromides could be formed. When alkynes with propargylic substituents are used, enhanced selectivity for formation of the Z-isomer is observed. When aryl acetylenes are used as the coupling partners, complete selectivity for the Z-isomer is obtained. A mechanism involving a cis or trans halometalation is invoked to explain formation of the observed products. The vinyl halides have been shown to be precursors to alpha-hydroxy ketones and cyclopentenones, and as coupling partners in Suzuki-type reactions.
Characterization of a novel â sheet-structured peptide for pretargeting immunotherapy of amyloidosis (2019)

3021 Introduction: Amyloid deposition in abdominothoracic organs and peripheral nerves results is the major pathology associated with a growing number of diseases known as amyloidosis. The extracellular deposition of amyloid results in the disruption of tissue architecture leading to organ dysfunction and ultimately death. Removal of amyloid would improve organ function, prolong patient survival, and enhance quality of life. Passive immunotherapy, is a viable method for effecting amyloid removal from the patient organs; however, data from recent clinical evaluation of two amyloid-reactive antibodies has demonstrated the difficulty in meeting the primary outcome measures of patient improvement. Therefore, creative alternatives are required to treat these patients.
To enhance the utility of one therapeutic mAb, 11-1F4 (Blood. 2010;116(13):2241-4), which has shown efficacy in ~ 60% of patients with light chain-associated (AL) amyloidosis, we have proposed a novel pretargeting approach using a “peptope” reagent that exploits multi-amyloid-reactive peptide combined with a high-affinity, linear epitope for 11-1F4, known as A12. Herein we describe the structure and amyloid reactivity of a novel peptope construct designated p97, that comprises a short amyloid-reactive peptide that, unlike previous variants, is predicted to adopt a β sheet secondary structure. This reagent may serve as a diagnostic imaging agent, when radiolabeled, and as a pretargeting therapeutic agent for enhancing 11-1F4-binding to many forms of amyloid.
Peptope p97 was synthetized as an all L-amino acid peptide with a single D-tyrosine residue at position 2 to allow radioiodination that was resistant to dehalogenation in vivo. Female mice (n = 3) with severe hepatosplenic serum amyloid protein A (AA) amyloidosis received ~10 µg of 125I-p93 IV (~10 µCi/µg) in the lateral tail vein. SPECT/CT images acquired at 4 h post injection to visualize the biodistribution of the reagent in these mice. Tissue biodistribution measurements indicated specific uptake in the liver (11% ID/g) and spleen (8.5% ID/g) as well as material in the kidney (~14 %ID/g) associated with catabolism. Microautoradiographic studies of formalin-fixed tissue sections revealed specific uptake in amyloid deposits.
These data indicate that, in addition to α-helix-structured amyloid reactive peptides, we have now demonstrated that peptides with a simpler β-sheet secondary structure, which may be more facile and inexpensive to generate can serve effectively as amyloid-targeting peptides. Furthermore, when associated with the A12 peptide we hypothesize that these peptides can be used a pretargeting agents for improving immunotherapy with the 11-1F4 antibody.
Feasibility of transanal endoscopic total mesorectal excision for rectal cancer: results of a pilot study (2016)

This pilot study showed that high-quality TME was possible in most patients without serious complications. Transanal TME for patients with rectal cancer may be feasible and safe, but further investigations are necessary to evaluate its long-term functional and oncologic outcomes and to clarify its indications.
Refining genetic associations in multiple sclerosis (2008)

Genome-wide association studies involve several hundred thousand markers and, even when quality control is scrupulous, are invariably confounded by residual uncorrected errors that can falsely inflate the apparent difference between cases and controls (so-called genomic inflation).1 As a consequence such studies inevitably generate false positives alongside genuine associations. By use of Bayesian logic and empirical data, the Wellcome Trust Case Control Consortium suggested that association studies in complex disease should involve at least 2000 cases and 2000 controls, at which level they predicted that p values of less than 5×10−7 would more commonly signify true positives than false positives.