gebhart/patrepeval huggingface dataset:
Enhancement of deep acceptor activation in semiconductors by superlattice doping (1996)

The thermal activation of acceptors in wide-gap semiconductors can be very low due to large acceptor activation energies. It is shown that superlattice doping, i.e., the composition modulation of a uniformly doped ternary semiconductor, can enhance the acceptor activation by more than one order of magnitude.
Ferrowax microvalves for fully automated serial dilution on centrifugal microfluidic platforms (2021)

We herein describe a centrifugal microfluidic system to accomplish a fully automated serial dilution. The liquid flow on the disc was regulated by utilizing ferrowax microvalves systematically integrated into the channels within specially designed metering structures. By opening the differently positioned microvalves through irradiation of IR laser to allow metering, the same amount of diluent was serially eluted to the dilution chamber from the same diluent chamber. After dilution, the diluted samples were automatically delivered to the respective final product chambers by appropriately opening or closing the microvalves in the connecting channels, followed by rotating the disc. Based on this unique design principle, six consecutive two-fold and 10-fold dilutions were successfully achieved, yielding excellent accuracy in a wide dynamic range up to six orders of magnitude. Very importantly, the overall serial dilution process, including the diluent addition, mixing, and product transfer steps, was completed very rapidly within 5 min, due to the minimized procedures enabled by the automated actuation of the ferrowax microvalves at the rationally designed positions. We expect our centrifugal microfluidic system would serve as a powerful elemental tool to realize fully automated diagnostic microsystems involving the serial dilution process.
Comparative Genomic Analysis and In Vivo Modeling of Streptococcus pneumoniae ST3081 and ST618 Isolates Reveal Key Genetic and Phenotypic Differences Contributing to Clonal Replacement of Serotype 1 in The Gambia (2017)

Streptococcus pneumoniae serotype 1 is one of the leading causes of invasive pneumococcal disease (IPD) in West Africa, with ST618 being the dominant cause of IPD in The Gambia. Recently however, a rare example of clonal replacement was observed, where the ST3081 clone of serotype 1 replaced the predominant ST618 clone as the main cause of IPD. In the current study, we sought to find the reasons for this unusual replacement event. Using whole-genome sequence analysis and clinically relevant models of in vivo infection, we identified distinct genetic and phenotypic characteristics of the emerging ST3081 clone. We show that ST3081 is significantly more virulent than ST618 in models of invasive pneumonia, and is carried at higher densities than ST618 during nasopharyngeal carriage. We also observe sequence type-specific accessory genes and a unique sequence type-specific fixed mutation in the pneumococcal toxin pneumolysin, which is associated with increased hemolytic activity in ST3081 and may contribute to increased virulence in this clone. Our study provides evidence that, within the same serotype 1 clonal complex, biological properties differ significantly from one clone to another in terms of virulence and host invasiveness, and that these differences may be the result of key genetic differences within the genome.
SecHLS (2023)

In their quest for further optimization, High-level synthesis (HLS) utilizes advanced automatic optimization algorithms to achieve lower implementation time/effort for even more complex designs. These optimization algorithms are for the HLS tools' backend stages, e.g., allocation, scheduling, and binding, and they are highly optimized for resources/latency constraints. However, current HLS tools' backend is unaware of designs' security assets, and their algorithms are incapable of handling security constraints. In this paper, we propose Secure-HLS (SecHLS), which aims to define underlying security constraints for HLS tools' backend stages and intermediate representations. In SecHLS, we improve a set of widely-used scheduling and binding algorithms by integrating the proposed security-related constraints into them. We evaluate the effectiveness of SecHLS in terms of power, performance, area (PPA), security, and complexity (execution time) on small and real-size benchmarks, showing how the proposed security constraints can be integrated into HLS while maintaining low PPA/complexity burdens.
Human papillomavirus infection in a woman with cervical dysplasia (1998)

L'observation Les examens cytologiques des frottis cervicovaginaux de Madame H.M., 38 ans, realises au cours de ces dernieres annees, ont mis en evidence des lesions de type Ascus (atypies malpighiennes de signification indeterminee). Madame H.M. est vue en consultation de gynecologie pour un controle en decembre 1997. Ses frottis sont anormaux avec un aspect de [...]
La presse et la vie littéraire sous le joug de la censure (1992)

info:eu-repo/semantics/published
Tensile Behavior of Ti,Mo-Added Low Carbon Steels with Interphase Boundary Precipitated Structures (2013)

Tensile behavior and structure-property relationship of ferritic steels with nano-sized carbide dispersion were invesigated using Ti-added steel and Ti,Mo-added low carbon steels. By austenitizing followed by isothermal heat treatment at 700°C, polygonal ferrites containing very fine carbides of TiC and (Ti,Mo) C were obtained in the Ti-added and the Ti,Mo-added steels, respectively. The size of such carbides was finer in the Ti,Mo-added steel than in the Ti-added steel at the same holding period of isothermal heat treatment. The results of tensile tests for these samples showed that the strength is higher as the diameter of the carbides is smaller. The structure-based strength calculation led to a good agreement with the experiments, when it was assumed that the Ashby-Orowan mechanism is dominant for precipitation strengthening of nano-sized alloy carbides. It was also suggested that a relatively large tensile ductility is related to enhanced recovery during the tensile deformation, accompanied with promotion of secondary slips or cross slips in a finer scale due to the nano-sized particles.
Free-electrons radiation in a photonic time crystal (2020)

We present novel radiation emission by free electrons moving in a spatiotemporally modulated medium, which in specific cases acts as a photonic time crystal. We obseive two regimes of radiation, subluminal and superluminal.
Probenpräparation für die Rückstreuelektronen-Kikuchi-Beugung (Electron Backscatter Diffraction, EBSD) / Specimen Preparation for Electron Backscatter Diffraction — Part I: Metals (2001)

Article Probenpräparation für die Rückstreuelektronen-Kikuchi-Beugung (Electron Backscatter Diffraction, EBSD) / Specimen Preparation for Electron Backscatter Diffraction — Part I: Metals was published on October 1, 2001 in the journal Practical Metallography (volume 38, issue 10).
Novel CRISPR-Associated Gene-Editing Systems Discovered in Metagenomic Samples Enable Efficient and Specific Genome Engineering for Cell Therapy Development (2021)

Abstract Gene-editing technology has revolutionized molecular therapeutics, enabling DNA-engineering-based approaches to treat disease. Despite this, development of medicines using gene editing has been hampered by technological, immunological, and legal limitations. We described previously the discovery of novel type II and type V gene-editing systems from metagenomic data, the characterization of these systems in vitro, and a demonstration of their activity in immortalized cell lines. Here, we substantially advance this work with three separate, novel gene-editing systems, demonstrating their utility for cell therapy development. We express and purify the nuclease components of both type II and type V effectors and show that all three systems are capable of reproducible, high-frequency gene editing in primary immune cells. In human T cells, disruption of the T cell receptor (TCR) alpha-chain constant region occurred in up to 95% of cells, both copies of the TCR beta-chain constant region in up to ~90% of cells, and beta-2 microglobulin in up to 95% of cells. Simultaneous double knock-out of TRAC and TRBC was obtained at an equal frequency. Gene editing with our systems had no effect on T cell viability. Further, we use our novel gene-editing systems to exploit homology-dependent DNA repair to integrate a CAR construct into the TCR alpha-chain locus (in up to ~60% of T cells), and demonstrate high-level CAR expression and antigen-dependent CAR-T cytotoxicity. Such robust editing activity at the TCR loci will permit efficient engineering and manufacture of allogeneic chimeric antigen receptor- (CAR) and TCR-based cell therapies. We next applied our novel gene-editing tools to NK cells and B cells. We achieved almost 100% gene disruption at the CD38 locus in NK cells and integrated a chimeric antigen receptor into the NK cell genome at a frequency of ~40%. Such CAR-NK cells displayed robust CAR-directed cellular cytotoxicity. B cell editing occurred in approximately 80% of target cells with successful transgene integration. Cas9 is notorious for tolerating mismatches and bulges in its target, resulting in high levels of unwanted DNA double-strand break formation. In contrast, interrogation of our gene editing systems using GUIDE-seq reveals no or very few off-target sites even at doses greatly in excess of those required for high-frequency gene editing. Finally, as our systems are taken from microbes found in environmental samples rather than from human pathogens, we expect pre-existing immunity to our nucleases will be quite rare. In all, we show that three new gene-editing systems have the activity, specificity, and translatability necessary for use in cell therapy development. Disclosures No relevant conflicts of interest to declare.
Degrees of Freedom of the Broadcast Channel with Hybrid CSI at Transmitter and Receivers (2017)

In general, the different links of a broadcast channel may experience different fading dynamics and, potentially, unequal or hybrid channel state information (CSI) conditions. The faster the fading and the shorter the fading block length, the more often the link needs to be trained and estimated at the receiver, and the more likely that CSI is stale or unavailable at the transmitter. Disparity of link fading dynamics in the presence of CSI limitations can be modeled by a multi-user broadcast channel with both non-identical link fading block lengths as well as dissimilar link CSIR/CSIT conditions. This paper investigates a MISO broadcast channel where some receivers experience longer coherence intervals (static receivers) and have CSIR, while some other receivers experience shorter coherence intervals (dynamic receivers) and do not enjoy free CSIR. We consider a variety of CSIT conditions for the above mentioned model, including no CSIT, delayed CSIT, or hybrid CSIT. To investigate the degrees of freedom region, we employ interference alignment and beamforming along with a product superposition that allows simultaneous but non-contaminating transmission of pilots and data to different receivers. Outer bounds employ the extremal entropy inequality as well as a bounding of the performance of a discrete memoryless multiuser multilevel broadcast channel. For several cases, inner and outer bounds are established that either partially meet, or the gap diminishes with increasing coherence times.
Time-resolved investigation of low-density plasma channels produced by a kilohertz femtosecond laser in air (2005)

By employing pump-probe back longitudinal diffractometry, the electron density and decay dynamics of a weak plasma channel created by a 1-KHz fs laser in air has been investigated. With ultrashort laser pulses of 50 fs and low energy of 0.6 mJ, we observe weak plasma channels with a length approximately 2 cm in air. An analytical reconstruction method of electron density has been analyzed, which is sensitive to the phase shift and channel size. The electron density in the weak plasma channel is extracted to be about 4 x 10(16) cm(-3). The diameters of the plasma channel and the filament are about 50 and 150 microm, respectively, and the measurable electron density can be extended to less than 10(15) cm(-3). Moreover, a different time-frequency technique called linearly chirped longitudinal diffractometry is proposed to time-resolved investigate ultrafast ionization dynamics of laser-irradiated gas, laser interaction with cluster beam, etc.
<title>Development of hybrid CMOS visible focal plane arrays at Rockwell</title> (2000)

Silicon-based hybrid CMOS visible focal plane array (FPA) technology is emerging as a strong contender for scientific applications that require broad spectral response with low noise, highly integrated functionality and radiation hardness. CMOS-based FPAs offer many advantages in high speed, low-noise detection and signal processing. As a high performance alternative to advanced CCD imaging arrays, the hybrid design enables independent optimization of the silicon detector array and silicon readout electronics. Multiplexer commonality with the instrument's IR channels is another attractive feature for integrators of sensor sites such as for hyperspectral spectrometers. In this paper, the technical merits of Rockwell's CMOS-based hybrid visible FPAs are described including key detector performance aspects, interface electronics requirements, radiation hardness and concomitant implications for diverse imaging applications. At this time we have developed 640 X 480 and 1024 X 1024 hybrid imagers with approximately equals 100% optical fill factor, high broadband QE spanning ultraviolet (UV) through near infrared (NIR), wide dynamic range, and high pixel operability. Dark current of approximately equals 0.01e-/sec and read noise approximately equals 6e- have been measured on one prototype 1024 X 1024 FPA that uses Hawaii readout integrated circuit (ROIC). Initial radiation data indicate a total ionization dose (TID) tolerance greater than 35 Krad for our standard CMOS process.
Novel model for mean stress effect on high-cycle fatigue performance of threaded fasteners (2020)

An experimentally validated fatigue model is proposed for the effect of mean stress on high-cycle fatigue performance of preloaded threaded fasteners. The proposed model is compared with commonly used models by Goodman, Gerber, Morrow, Soderberg, and ASME-Elliptic (ASME B106.1M:1985). Fatigue S-N curves are experimentally generated at various mean stress levels for both commercially available (CA) threaded fasteners (Class 10.9 and 12.9), as well as ultra-high strength (UHS) fasteners (Class 14.8, 15.8 and 16.8). Proposed model prediction and test data show that at high levels of fastener mean stress, the bainite microstructure of the ultra-high strength fasteners has a clear detrimental effect on fastener fatigue performance, as compared to the martensite microstructure of commercially available low carbon steel fasteners. Further discussion of test-validated model predictions and conclusions are provided.
Structure of immunoglobulin A. I. Interchain disulfide bridges of a γAl myeloma protein (1971)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructure of immunoglobulin A. I. Interchain disulfide bridges of a γAl myeloma proteinC. Wolfenstein, Blas Frangione, E. Mihaesco, and E. C. FranklinCite this: Biochemistry 1971, 10, 22, 4140–4145Publication Date (Print):October 1, 1971Publication History Published online1 May 2002Published inissue 1 October 1971https://pubs.acs.org/doi/10.1021/bi00798a020https://doi.org/10.1021/bi00798a020research-articleACS PublicationsRequest reuse permissionsArticle Views34Altmetric-Citations16LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Information Model for Abstraction and Control of TE Networks (ACTN) (2018)

This draft provides an information model for Abstraction and Control
of Traffic Engineered Networks (ACTN).
Regulation of virulence in Francisella tularensis by small non-coding RNAs (2011)

Abstract Using a cDNA cloning and sequencing approach we have shown that Francisella tularensis expresses homologues of several small RNAs(sRNAs) that are well-conserved among diverse bacteria. We have also discovered two abundant putative sRNAs that share no sequence similarity or conserved genomic context with any previously annotated regulatory transcripts. Deletion of either of these two loci led to significant changes in the expression of several mRNAs that likely include the cognate target(s) of these sRNAs. Deletion of these sRNAs did not, however, significantly alter F. tularensis growth under various stress conditions in vitro, its replication in murine cells, or its ability to induce disease in a mouse model of F. tularensis infection.
Data from A Tricin Derivative from <i>Deschampsia antarctica</i> Desv. Inhibits Colorectal Carcinoma Growth and Liver Metastasis through the Induction of a Specific Immune Response (2023)

&lt;div&gt;Abstract&lt;p&gt;In colorectal carcinoma patients, distant metastatic disease is present at initial diagnosis in nearly 25% of them. The majority of patients with metastatic colorectal carcinoma have incurable disease; therefore, new therapies are needed. Agents derived from medicinal plants have already demonstrated therapeutic activities in human cancer cells. Antartina is an antitumor agent isolated from &lt;i&gt;Deschampsia antarctica&lt;/i&gt; Desv. This study aimed to evaluate the antitumor properties of Antartina in colorectal carcinoma models. We used human and murine colorectal carcinoma cell lines for investigating proliferation, apoptosis, and cell-cycle effects of Antartina therapy &lt;i&gt;in vitro&lt;/i&gt;. Avatar and immunocompetent colorectal carcinoma animal models were applied for evaluating the effects of Antartina &lt;i&gt;in vivo&lt;/i&gt;. Immune response against colorectal carcinoma model was investigated using CTL assay, analyzing dendritic cell activation and intratumor T-cell subpopulation, and by tumor rechallenge experiments. Antartina inhibits &lt;i&gt;in vitro&lt;/i&gt; human colorectal carcinoma cell proliferation; however, &lt;i&gt;in vivo&lt;/i&gt; experiments in Avatar colorectal carcinoma model Antartina display a limited antitumor effect. In an immunocompetent colorectal carcinoma mice model, Antartina potently inhibited tumor growth and liver metastases, leading to complete tumor regressions in &gt;30% of mice and increased animal survival. In addition, Antartina induced a potent specific cytotoxic T-cell response against colorectal carcinoma and a long-lasting antitumor immunity. Interestingly, Antartina increased tumor immunogenicity and stimulated dendritic cell activation. No toxic effects were observed at the doses employed. Our findings showed that Antartina has the ability to induce antitumor immunity against colorectal carcinoma and can be used to develop new tools for the treatment of colorectal carcinoma. &lt;i&gt;Mol Cancer Ther; 17(5); 966–76. ©2018 AACR&lt;/i&gt;.&lt;/p&gt;&lt;/div&gt;
Sgp130, Natural Inhibitor of Soluble Interleukin-6 Transsignaling, is Increased in Chronic Liver Diseases (2006)

info:eu-repo/semantics/published
Naloxone regulates the differentiation of neural stem cells via a receptor‐independent pathway (2020)

The abilities of opioids to activate downstream signaling pathways normally depend on the binding between opioids and their receptors. However, opioids may also function in a receptor-independent manner, especially in neural stem cells (NSCs) in which the expression of opioid receptors and endogenous opioid agonists is low. When two opioids, morphine and naloxone, were used during the early stage of NSC differentiation, increased neurogenesis was observed. However, naloxone methiodide, a membrane impenetrable analog of naloxone, did not affect the NSC differentiation. The abilities of morphine and naloxone to facilitate neurogenesis were also observed in opioid receptor-knockout NSCs. Therefore, morphine and naloxone promote neurogenesis in a receptor-independent manner at least during the early stage. In addition, the receptor-independent functions of opioids were not observed in methylcytosine dioxygenase ten-eleven translocation 1 (Tet1) knockout NSCs. When the expression of opioid receptors increased and the expression of Tet1 decreased during the late stage of NSC differentiation, morphine, but not naloxone, inhibited neurogenesis via traditional receptor-dependent and miR181a-Prox1-Notch-related pathway. In summary, the current results demonstrated the time-dependent effects of opioids during the differentiation of NSCs and provided additional insight on the complex functions of opioids.
Synthesis and characterization of 3‐phenylethynyl endcapped matrix resins (1995)

Abstract The synthesis of 3‐phenylethynylphenol, and its applicability as a high temperature cross‐linking endcap for high T g polyarylene ethers is described. It was synthesized in high yields and purity using the palladium catalyzed coupling reaction between the protected 3‐bromo or iodo phenol and phenylacetylene. The yield of the reaction was found to be highly dependent on the structure of the halide used, the reaction temperature, and the concentration of phenylacetylene. The use of the protected phenol in the palladium catalyzed reaction was also extended to the high yield synthesis of 3‐ethynylphenol and protected 4‐ethynylphenols. The complete synthesis of 3‐phenylethynylphenol, 3‐ethynylphenol, and protected 4‐ethynylphenol in high yields has been demonstrated and is discussed herein. Three new phenylethynyl functionalized arylene ether matrix resins have been synthesized in high yields and purity by reacting 3‐phenylethynylphenol with 4,4′‐dichlorodiphenyl sulfone, 4,4′‐difluorobenzophenone, and bis(4‐fluorophenyl)phenyl phosphine oxide, via nucleophilic poly(arylene ether) synthesis conditions. These low molecular weight materials undergo thermally induced chain extension/branching to yield an insoluble three‐dimensional network at reaction temperatures of around 380°C. The low molecular weight arylene ethers endcapped with the phenylethynyl group demonstrate excellent flow characteristics and a wide processing window of about 250°C. Crosslinking of the 4,4′‐bis(3‐phenylethynyl phenoxy)diphenyl sulfone system for 30 min at 350°C in air afforded a T g value of 265°C by differential scanning calorimetry measurements. Trace metal analysis for palladium and copper showed absence of these metals that would otherwise detract from the excellent thermal stability. The synthesis and characterization of these phenylethynyl endcapped arylene ether matrix resins is discussed. © 1995 John Wiley &amp; Sons, Inc.
Supplementary Figure 5A-5E from Discovery and Characterization of Novel Mutant FLT3 Kinase Inhibitors (2023)

Supplementary Figure 5A-5E from Discovery and Characterization of Novel Mutant FLT3 Kinase Inhibitors