gebhart/patrepeval huggingface dataset:
A versatile gene trap to visualize and interrogate the function of the vertebrate proteome (2011)

We report a multifunctional gene-trapping approach, which generates full-length Citrine fusions with endogenous proteins and conditional mutants from a single integration event of the FlipTrap vector. We identified 170 FlipTrap zebrafish lines with diverse tissue-specific expression patterns and distinct subcellular localizations of fusion proteins generated by the integration of an internal citrine exon. Cre-mediated conditional mutagenesis is enabled by heterotypic lox sites that delete Citrine and "flip" in its place mCherry with a polyadenylation signal, resulting in a truncated fusion protein. Inducing recombination with Cerulean-Cre results in fusion proteins that often mislocalize, exhibit mutant phenotypes, and dramatically knock down wild-type transcript levels. FRT sites in the vector enable targeted genetic manipulation of the trapped loci in the presence of Flp recombinase. Thus, the FlipTrap captures the functional proteome, enabling the visualization of full-length fluorescent fusion proteins and interrogation of function by conditional mutagenesis and targeted genetic manipulation.
700 kVA/L power density IGBT module for xEV power train (2017)

This paper demonstrates the new 1200 A / 750 V IGBT module which has the best in class power density of 700 kVA/L for xEV power train. The new IGBT module targets to achieve the 200 kW class motor. The key trend of the power module for xEV is high power density, lower power dissipation, downsizing, low thermal resistance and high reliability. In order to satisfy these requirements, the new IGBT module is adapted the lead frame technology, new Reverse Conducting (RC)-IGBT featuring Fuji's 7th-gen IGBT technology and Al direct liquid cooling structure. As a result, the best performance of power density as 700 kVA/L is achieved.
Promoter of the pertussis toxin operon and production of pertussis toxin (1987)

Pertussis toxin (PT), the major virulence factor of Bordetella pertussis, is composed of five different subunits whose genes are organized as an operon. We report the mapping of the promoter region of the PT operon and show that this promoter is only weakly active in Escherichia coli. Bordetella parapertussis and Bordetella bronchiseptica, which do not produce any PT, are shown to have a weaker promoter sequence for this operon and not to produce any detectable PT mRNA. We show that transcription of the PT operon in B. pertussis was constant throughout until the late stationary phase, when transcription significantly decreased. Analysis of the transposon Tn5 mutant BP347 showed that the product of the vir locus was required for transcription of the PT operon. Characterization of the Tn5 mutant BP356 showed that subunit S3 was required for the release of PT into the extracellular medium.
Better ARP handling with InSPired SDN switches (2016)

SDN research is moving towards enabling the delegation of parts of control functions to the network elements. In recent work we analytically evaluated the benefit in terms of ARP data traffic load reduction as well as control channel interaction reduction when delegating the generation of ARP replies. In this paper we describe the corresponding control program and network configuration to handle ARP traffic in a software-defined network leveraging programmable in-switch packet generation capabilities. We also discuss potential optimizations and implementation options.
Enhanced probabilistic packet marking for IP traceback (2005)

A novel mechanism based on probabilistic packet marking (PPM) for IP traceback is presented. Our proposal enhances the performance of PPM in the following aspects. First, PPM can effectively trace denial of service (DoS) attacks and small-scale distributed DoS (DDoS) attacks only while our proposal may also be used to tackle large-scale DDoS attacks. Second, our scheme eliminates a serious vulnerability of PPM, i.e., spoofed marking inscribed by the attacker intentionally. Third, by optimizing the marking probability and refining the marking mechanism, our scheme can significantly reduce the number of packets required for path reconstruction. In comparison with PPM, as many as 41.31% of marked packets required for a single path reconstruction may be reduced using our scheme.
Plasmonic Nano-Focused Four-Wave Mixing (2015)

We demonstrate efficient and broadband nonlinear nano-optics in plasmonic nano-focusing with nanometer localized four-wave mixing. We achieve deterministic optical control and nano-spectroscopic imaging of graphene as the basis for extension of multidimensional spectroscopy to the nanoscale.
Validity of proposed DSM-5 diagnostic criteria for nicotine use disorder: results from 734 Israeli lifetime smokers (2013)

All findings address previous concerns about the DSM-IV nicotine diagnosis and its criteria and support the proposed changes for DSM-5 NUD, which should result in improved diagnosis of nicotine disorders.
Optical properties of a new IR converter for laser beam analysis (2007)

This work presents the temporal and spacial resolution of a new infrared (IR) converter based on thermal radiation emission. Using this converter, it is possible to measure the intensity distribution of laser beams with a wavelength between 1 and 20 μm. For this purpose, the laser radiation (for example, 10.6 μm) is converted into a wavelength coverage of 800–1100 nm. In the actual converter thin metal foils provide the basis of this method. The metal foils are heated to a temperature of 600–800 K. The emitted radiation of the foils defers into the near-infrared (NIR) area, thus enabling detection by camera systems based on silicate. Additional heat input of the laser results in a local temperature increase, and then the increase in radiation intensity can be measured. Typical thicknesses of converter metal foils are <5μm. Foil materials with a low thermal conductivity, good absorption of the measured laser beams, and a high melting temperature are particularly suitable. These parameters are well shown by using stainless steels, such as INOX (stainless steel 1.4310 CrNi steel). Using this material, it is possible to gain a maximum spatial resolution of 250 μm and a temporal resolution of 12.5 Hz, by a measurement range from 1 to 100 W/cm2. The maximum measured intensity is 125 W/cm2.
LTE performance in the airport surface channel (2011)

Presents a collection of slides covering the following topics: LTE system model: airport surface area channel; PHY processing; UL SC-FDMA and non-stationary TDL channel model.
Dynamic Binary Countdown for Massive IoT Random Access in Dense 5G\n Networks (2019)

Massive connectivity for Internet of Things applications is expected to\nchallenge the way access reservation protocols are designed in 5G networks.\nSince the number of devices and their density are envisioned to be orders of\nmagnitude larger, state-of-the-art access reservation, Random Access (RA)\nprocedure, might be a bottleneck for end-to-end delay. This would be especially\nchallenging for burst arrival scenarios: Semi-synchronous triggering of a large\nnumber of devices due to a common event (blackout, emergency alarm, etc.). In\nthis article, to improve RA procedure scalability, we propose to combine Binary\nCountdown Contention Resolution (BCCR) with the state-of-the-art Access Class\nBarring (ACB). We present a joint analysis of ACB and BCCR and apply a\nframework for treating RA as a bi-objective optimization, minimizing the\nresource consumption and maximizing the throughput of the procedure in every\ncontention round. We use this framework to devise dynamic load-adaptive\nalgorithm and simulatively illustrate that the proposed algorithm reduces the\nburst resolution delay while consuming less resources compared to the\nstate-of-the-art techniques.\n
Content-based Hierarchies in DHT-based Peer-to-Peer Systems (2006)

Peer-to-peer systems allowing fast and easy search for information shared by the users of a communication network, are considered to have a huge impact on next generation mobile service platforms. To support applications that employ content categories or limit services to certain contexts more efficiently, we describe a hierarchical P2P system in this paper. In particular, our system introduces hierarchies in a DHT-based P2P system to allow topic-based queries. Based on the query statement, which is based on XPath, queries can traverse the hierarchy if the content is not found in one topic-space. Examples illustrate how our concept is used for file sharing and location-aware applications
Mutant Dyskerin Ends Relationship with Telomerase (1999)

It has been known for some time that individuals with the X-linked human disease, dyskeratosis congenita (DKC) carry mutations in the gene encoding dyskerin. It has been proposed that DKC may be a ribosomal RNA (rRNA) disorder as dyskerin is similar to a yeast protein that is involved in the production of rRNA In a Perspective,
 Shay and Wright
 discuss new findings showing that dyskerin interacts with telomerase (the enzyme needed to maintain the ends of chromosomes) and stabilizes the RNA component of this enzyme. It turns out that DKC is a telomere maintenance disorder, which explains the age-related features of the disease (telomeres shorten during the normal aging process).
Metal-semiconductor-metal traveling-wave photodetectors (2001)

We demonstrate a novel type of traveling wave photodetector: "metal-semiconductor-metal traveling-wave photodetecor" (MSM-TWPD). The demonstrated devices were fabricated using low-temperature grown GaAs (LTG-GaAs). In order to achieve high internal quantum efficiency, the narrow spacing between electrodes was fabricated by the self-aligned process without e-beam lithography. Electro-optical sampling measurement results at different optical pumping levels are reported. Ultrahigh bandwidth (0.8-ps, 570-GHz transform bandwidth) performance was observed even under high optical power illumination (/spl sim/1.8 mW) with 8.1% net quantum efficiency. Compared with a LTG-GaAs-based p-i-n TWPD and vertically illuminated MSM photodetector (PD), this novel TWPD has higher output saturation current with near terahertz electrical bandwidth, better quantum efficiency, and can be easily fabricated and integrated with other microwave devices. It thus promises the application in high-power distributed PD array or terahertz signal generation.
Bone Tissue Engineering and Regeneration: From Discovery to the Clinic—An Overview (2011)

A National Institutes of Health sponsored workshop "Bone Tissue Engineering and Regeneration: From Discovery to the Clinic" gathered thought leaders from medicine, science, and industry to determine the state of art in the field and to define the barriers to translating new technologies to novel therapies to treat bone defects. Tissue engineering holds enormous promise to improve human health through prevention of disease and the restoration of healthy tissue functions. Bone tissue engineering, similar to that for other tissues and organs, requires integration of multiple disciplines such as cell biology, stem cells, developmental and molecular biology, biomechanics, biomaterials science, and immunology and transplantation science. Although each of the research areas has undergone enormous advances in last decade, the translation to clinical care and the development of tissue engineering composites to replace human tissues has been limited. Bone, similar to other tissue and organs, has complex structure and functions and requires exquisite interactions between cells, matrices, biomechanical forces, and gene and protein regulatory factors for sustained function. The process of engineering bone, thus, requires a comprehensive approach with broad expertise. Although in vitro and preclinical animal studies have been pursued with a large and diverse collection of scaffolds, cells, and biomolecules, the field of bone tissue engineering remains fragmented up to the point that a clear translational roadmap has yet to emerge. Translation is particularly important for unmet clinical needs such as large segmental defects and medically compromised conditions such as tumor removal and infection sites. Collectively, manuscripts in this volume provide luminary examples toward identification of barriers and strategies for translation of fundamental discoveries into clinical therapeutics.
Relationship between Yersinia enterocolitica antibody level and bacterial shedding after challenge in previously immunized pigs (2013)

AsymptomaticcarriersofYersiniaenterocoliticaareoftenfoundinpigswhicharethemainreservoir and source of human infection. Carrying of that bacteria is usually connected with a long-term shedding and microbiological contamination of the environment. Thus, the aim of this study was to determine the effectofimmunizationwitha suspensionofselectedstrainsofY.enterocoliticaonthedurationofpathogen sheddinginexperimentallyinfectedpigs. Fifteenpigsweredividedintothreegroups,twoexperimentalandonecontrol.Immunizingsuspension wasadministeredsubcutaneouslyindosesof2 ml(groupI)and5 ml(groupII)withthedensityof2.7◊10 9 CFU/mlofformaldehydeinactivatedcellssuspendedinphosphate-bufferedsalineintwoinjectionswithin an interval of two weeks. Pigs were experimentally infected with a 10 ml dose of a pathogenic strain of Y.enterocoliticaO:3withthedensityof2.7◊10 9CFU/mltwoweeksafterimmunization. Subcutaneous immunization stimulated higher antibody levels in group II, which was administered ahigherinoculantdoseof5 mlsuspensionwiththedensityof2.7◊10 9CFU/ml.Inthisgroup,shedding was not reported in two out of five pigs, and the period of pathogen excretion was shorter in comparison withgroupI andthecontrolgroup.ExperimentalimmunizationagainstinfectionscausedbyY.enteroco liticadidnotpreventpathogenshedding,butmerelylimitedtheintensityanddurationofbacterialexcre tion.
Modelling Behaviour of a Carbon Epoxy Composite Exposed to Fire: Part II—Comparison with Experimental Results (2017)

Based on a phenomenological methodology, a three dimensional (3D) thermochemical model was developed to predict the temperature profile, the mass loss and the decomposition front of a carbon-reinforced epoxy composite laminate (T700/M21 composite) exposed to fire conditions. This 3D model takes into account the energy accumulation by the solid material, the anisotropic heat conduction, the thermal decomposition of the material, the gas mass flow into the composite, and the internal pressure. Thermophysical properties defined as temperature dependant properties were characterised using existing as well as innovative methodologies in order to use them as inputs into our physical model. The 3D thermochemical model accurately predicts the measured mass loss and observed decomposition front when the carbon fibre/epoxy composite is directly impacted by a propane flame. In short, the model shows its capability to predict the fire behaviour of a carbon fibre reinforced composite for fire safety engineering.
Limitations on HTS single flux quantum logic (1999)

There is widespread interest in transferring Single Flux Quantum (SFQ) logic from niobium technology to a suitable HTS technology. The higher operating temperature results in increased noise and associated errors. We have developed numerical methods to study noise induced errors and how they depend on circuit parameters. A simple picture holds for all the circuits studied to date. The error probability shows an error function (integrated Gaussian) distribution. The width of the distribution is circuit dependent, but is 1 to 4 times the noise contribution of a single junction, and the noise free (deterministic) margin corresponds to the point where the error probability is 0.5. The distributions are frequently asymmetric, and minimum error rate does not occur for a design centered between the deterministic margins. A stochastic optimizer has been developed that allows us reoptimize circuits for conditions when noise induced margin narrowing is important. We have used this to study the influence of layout induced stray inductance on the feasibility of HTS circuits using the T flip-flop as an example. Stray inductance appears to be as important as junction reproducibility, and significant improvements are needed to allow high operating temperatures.
Dyeing para‐aramid fabrics in supercritical carbon dioxide with pretreatment of nonthermal plasma induced oil polymers (2022)

Abstract It has been a great challenge to effectively dye para‐aramids. This study systematically evaluated the dyeing of textile fabrics made of woven continuous filament, highly crystalline para‐aramids in supercritical carbon dioxide (ScCO 2 ) at various combinations of temperature ( T = 80–160°C) and pressure ( P = 10–20 MPa), focusing on our new pretreatment method of using nonthermal plasma (NTP) to induce oil polymers on the surface of the fabrics for the enhancement of dyeing. We found that, without any pretreatment, the specific para‐aramid fabric used in this study is undyeable (dyed K / S value of ~0.5), even after an NTP surface treatment and undergoing a dyeing process in ScCO 2 , due to its high crystallinity and chemical inertness. Pretreating the para‐aramids with soybean oil, acrylated epoxidized soybean oil (AESO), or a mixture of soybean oil and acrylic acid (AA/Soy) followed by an NTP treatment significantly improved the dyeing color strength, as measured by K / S values up to 9.7. In these cases, AESO and AA/Soy provided comparable results, better than that obtained with soybean oil. The NTP treatment is essential in inducing a cross‐linked polymer network in situ, anchoring the para‐aramids with covalent bonds and also providing functional groups that bind the dye. The new dyeing method did not compromise the strength of the para‐aramids. Further, dyes soluble in ScCO 2 should be selected, since the use of dyes insoluble in ScCO 2 would require the addition of co‐solvents and other auxiliary chemicals, which complicates the downstream separation of these chemicals and decreases the benefit of ScCO 2 dyeing.
ChemInform Abstract: Organocatalyzed Asymmetric Mannich Reactions (2008)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Poster: Memristive Systems (2012)

This chapter discusses in detail a RS HfO2-based memory cell with a metal-insulator-metal (MIM) vertical structure in which the HfO2 was deposited by Atomic Layer Deposition (ALD). The author carries out a statistic study on the effect of the HfO2 deposition process conditions on the resistive switching behavior. The chapter investigates the transient currents during 5-ns resistive switching operations. It proposes a short voltage pulse induced electroforming. The chapter models the current transport in metal-oxide-metal structures to gain an in-depth insight into the electroforming process. It reports the fabrication and electrical characterization of two-terminal multistate memory devices based on VO2/TiO2 thin film microcantilevers. The chapter reports on the feasibility of the Ge2Sb1Te4, Ge3Sb4Te8 and Ge2Sb2Te4 alloys and the effect of vacancies on their physical properties. It aims to determine. Controlled Vocabulary Terms atomic layer deposition; cantilevers; MIM structures
A holistic approach to challenges in Industry 4.0 (2018)

Durante as últimas décadas, uma série de Tecnologias de Informação e Comunicação (TICs) de ponta ganhou força na indústria e na academia. Incluindo várias tecnologias, como por exemplo a Internet das coisas, computação em nuvem e sensores inteligentes. Como um paradigma digital que promove o planejamento e o controle descentralizados, juntamente com a personalização em massa na manufatura, a Indústria 4.0 está atualmente abrindo caminho para uma quarta revolução industrial. Neste artigo, apresentamos desafios importantes neste contexto de TICs e personalização de massa da Indústria 4.0, ambicionando a realização de objetivos de produção conflitantes de preço e customização. Em nossa visão holística, discutimos esses desafios sob os prismas da ciência da computação e da engenharia de produção.
Insulin‐Like Growth Factor I in the Anterior Pituitary of the Clawed Frog Xenopus laevis: Immunocytochemical and Autoradiographic Indication for a Paracrine Action and Corelease with Prolactin (2000)

Insulin-like growth factor I (IGF-I) and its receptor are present in human and rat anterior pituitary. However, few data exist on the potential presence of IGF-I or its receptor in the non-mammalian pituitary and the cellular sites of IGF-I production have not been identified in any species. Thus, we investigated the anterior pituitary of the clawed frog Xenopus laevis which is widely used to study growth and differentiation. The study was performed with antisera against mammalian insulin-like growth factor I (IGF-I), prolactin (PRL) and growth hormone (GH) using immunohistochemical and immunocytochemical techniques. IGF-I binding was determined by in-vitro receptor autoradiography. The PRL-and GH-immunoreactive cells exhibited distinct distribution patterns. Neither at the light nor the electron microscopical level any colocalization of PRL-and GH-immunoreactivities was apparent. The PRL-immunoreactive cells exhibited round granules of medium electron density (mean diameter: 312 nm) and the GH-immunoreactive cells spherical granules of medium electron density (mean diameter: 165 nm). By the use of serial semithin sections IGF-I-immunoreactivity was exclusively located in PRL-immunoreactive cells. At the ultrastructural level, IGF-I-immunoreactivity was confined to the secretory granules in coexistence with PRL-immunoreactivity using the double labelling immunogold technique. Specific IGF-I binding sites were localized throughout the pituitary. The results provide evidence for a concomitant release of PRL and IGF-I and suggest autocrine/paracrine actions of IGF-I in the anterior pituitary.
Ultrathin Metal/Amorphous-Silicon/Metal Diode for Bipolar RRAM Selector Applications (2014)

We propose a novel metal/silicon/metal (MSM) selector using ultrathin undoped amorphous silicon (a-Si) for resistive-RAM selector application. The new selector behaves as a bidirectional diode, showing a high current drive (~2.2 MA/cm) <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> , high selectivity (~240 for 1/2 bias), fast switching speed , and excellent endurance ( at target drive current). The doping-free a-Si structure alleviates the dopant-induced variability concerns for ultrascaled devices and eliminates the need for a dopant-activation anneal. Circuit simulations show feasibility of 1-Mb array, with over 25% read margin and 70% write margin, when using the new MSM structure as a selector for a HfO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> -based resistive switching memory element.