gebhart/patrepeval huggingface dataset:
Magnetic Resonance Imaging-Guided Delivery of Adeno-Associated Virus Type 2 to the Primate Brain for the Treatment of Lysosomal Storage Disorders (2010)

Gene replacement therapy for the neurological deficits caused by lysosomal storage disorders, such as in Niemann-Pick disease type A, will require widespread expression of efficacious levels of acid sphingomyelinase (ASM) in the infant human brain. At present there is no treatment available for this devastating pediatric condition. This is partly because of inherent constraints associated with the efficient delivery of therapeutic agents into the CNS of higher order models. In this study we used an adeno-associated virus type 2 (AAV2) vector encoding human acid sphingomyelinase tagged with a viral hemagglutinin epitope (AAV2-hASM-HA) to transduce highly interconnected CNS regions such as the brainstem and thalamus. On the basis of our data showing global cortical expression of a secreted reporter after thalamic delivery in nonhuman primates (NHPs), we set out to investigate whether such widespread expression could be enhanced after brainstem infusion. To maximize delivery of the therapeutic transgene throughout the CNS, we combined a single brainstem infusion with bilateral thalamic infusions in naive NHPs. We found that enzymatic augmentation in brainstem, thalamic, cortical, as well subcortical areas provided convincing evidence that much of the large NHP brain can be transduced with as few as three injection sites.
Leucascandrolide B, a New 16-Membered, Extensively Methyl-Branched Polyoxygenated Macrolide from the Calcareous SpongeLeucascandra caveolata from Northeastern Waters of New Caledonia (1999)

We report a novel, extensively methyl-branched and polyoxygenated 16-membered macrolide, leucascandrolide B (=(1R*,5S*,7R*,9S*,13R*,14R*,15R*,11Z)-5-[(E)-1,4-dimethylhept-5-enyl]-1,7,9-trihydroxy-11,14-dimethyl-3-oxo-4,17-dioxabicyclo[11.3.1]heptadec-11-en-15-yl] carbamate; 2), isolated from the calcareous sponge Leucascandra caveolata Borojevic and Klautau from the northeastern coast of New Caledonia. The NMR structural assignments were corroborated by semisynthetic derivatives of 2, the functional groups and atom connectivity by derivatives 4 – 6 (obtained by acetylation, oxidation, and elimination-solvation, resp.), and the relative configurations by derivative 7 (obtained by carbonation). The preferred conformation of 2, centered on a chair-like tetrahydro-2H-pyran ring formed by intramolecular acetalization, was derived from molecular mechanics and semiempirical calculations which were in agreement with all NMR data. A dilactone `dimeric' structure 3, indistinguishable from 2 on the basis of all above data, was ruled out by tandem MS-MS experiments.
Static determination of allocation rates to support real-time garbage collection (2005)

While it is generally accepted that garbage-collected languages offer advantages over languages in which objects must be explicitly deallocated, real-time developers are leery of the adverse effects a garbage collector might have on real-time performance. Semiautomatic approaches based on regions have been proposed, but incorrect usage could cause unbounded storage leaks or program failure. Moreover, correct usage cannot be guaranteed at compile time. Recently, real-time garbage collectors have been developed that provide a guaranteed fraction of the CPU to the application, and the correct operation of those collectors has been proven, subject only to the specification of certain statistics related to the type and rate of objects allocated by the application. However, unless those statistics are provided or estimated appropriately, the collector may fail to collect dead storage at a rate sufficient to pace the application's need for storage. Overspecification of those statistics is safe but leaves the application with less than its possible share of the CPU, which may prevent the application from meeting its deadlines.In this paper we present a static analysis to bound conservatively an application's allocation rate. The analysis is based on a data flow framework that requires interprocedural evaluation. We present the framework and results from analyzing some Java benchmarks. Because static analysis is necessarily conservative, we also present measurements of our benchmarks' actual allocation rates.Our work is a necessary step toward making real-time garbage collectors attractive to the hard-real-time community. By guaranteeing a bound on statistics provided to a real-time collector, we can guarantee the operation of the collector for a given application.
High-Q Inductors for Realization of Low Insertion Loss Baluns and Couplers Integrated in Thin Film Multilayer MCM-D Technology for Wireless Applications (2000)

This paper presents spiral inductors with quality factors of more than 100 in a thin film muli-layer MCM-D technology. Together with other passive components, such as capacitors, resistors or interconnection lines, these spiral inductors are used to realize integrated baluns and various types of couplers for front-end systems for wireless application. Because o f the high-Q of the inductors and the high overall performance of the integrated passive components in general, the insertion loss of the baluns and couplers is kept very low. This, together with the enhanced miniaturization, leads to higher packaging densities and lower cost of wireless applications.
Noninvasive measurement of transmural gradients in myocardial strain with MR imaging. (1991)

A method is presented for performing high-resolution strain measurements by using magnetic resonance (MR) tagging. Multispectral radiofrequency pulses are used to produce tagging grids from which strain estimates are obtained with a resolution of 2 mm. A tag detection algorithm is presented that measures the center of a tag line with a precision that ranges from 0.1 to 0.2 mm over the systolic interval. With this method, a transmural gradient in the strain of a normal dog heart was detected.
Improved cancer coping from a web‐based intervention for prostate cancer survivors: A randomized controlled trial (2021)

The findings suggest PROGRESS may improve certain aspects of adaptive coping among PCa survivors that use the website, but does not adequately address the remaining coping and psychosocial domains. Additional research is needed to better understand the gaps in intervention delivery contributing to low engagement and poor improvement across all domains of functional quality of life and adaptive coping.
Minimally Invasive Surgical Management of Ureteropelvic Junction Obstruction: Laparoscopic and Robot-Assisted Laparoscopic Pyeloplasty (2005)

No AccessJournal of UrologyAdult Urology: Urological Survey: Urolithiasis, Endourology And Laparoscopy1 Mar 2005Minimally Invasive Surgical Management of Ureteropelvic Junction Obstruction: Laparoscopic and Robot-Assisted Laparoscopic Pyeloplasty Ralph V. ClaymanM.D. Ralph V. ClaymanRalph V. Clayman More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)60363-1AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "Minimally Invasive Surgical Management of Ureteropelvic Junction Obstruction: Laparoscopic and Robot-Assisted Laparoscopic Pyeloplasty." The Journal of Urology, 173(3), p. 868 © 2005 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 173Issue 3March 2005Page: 868 Advertisement Copyright & Permissions© 2005 by American Urological Association, Inc.MetricsAuthor Information Ralph V. Clayman More articles by this author Expand All Advertisement Loading ...
Identification of Vehicular Impact Conditions Associated with Serious Ran-off-Road Crashes (2010)

This report quantifies the characteristics of ran-off-road crashes and identifies appropriate impact conditions for use in full-scale crash testing. Many of the decisions related to design guidelines and policies can benefit from better information on the impact conditions of ran-off-road crashes. The report will be of particular interest to personnel responsible for the design of roadside safety features.
Fail output algorithm of vision sensing (2002)

Vision sensor has become a recent adoption as a potentially effective means for external sensing for automobiles. In the field of external sensing, a number of options for lane detection, distance recognition and other parameters have been proposed. Among others, the most important and yet difficult challenge is to establish a failure detection method for each recognition algorithm in order to prevent an error in system's detection under actual conditions where the sensor is used. This paper reports on one version for detecting failure in the vision sensor that recognizes obstacles in front of the vehicle in motion. The study was based on an innovative algorithm that judges the limit of vehicle recognition using a stereo vision camera under adverse weather conditions, in particular in the rain.
Abstract 2703: Loss of dependence on EGFR signaling by upregulation of Mig6 confers drug resistance to erlotinib (2010)

Abstract We sought to elucidate mechanisms of resistance to epidermal growth factor receptor (EGFR)-targeted therapies and to identify markers that could better distinguish resistant from sensitive patients. Here we show that mitogen-inducible gene 6 (Mig6), a negative regulator of EGFR, was selectively upregulated during the evolution of resistance to the EGFR tyrosine kinase inhibitor (TKI) erlotinib in lung and head and neck cancer cell lines. Mig6 was a major determinant of EGFR activity in panels of cancer cell lines of different tissue origins, and in direct validation of approved human use, in a unique collection of directly xenografted human lung and pancreas tumors. Our work highlights the importance of cellular negative regulators of EGFR in predicting and subverting TKI responsiveness, and identifies the ratio of Mig6 to EGFR, rather than the absolute expression of either protein alone, to be a predictor of tumor cell response to erlotinib. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 2703.
Computational Study of Temperature Effects on MOSFET Channel Material Benchmarking (2020)

Temperature (T) effects on MOSFET channel material benchmarking are explored using atomistic quantum and Monte Carlo simulation. Performance metrics such as OFF-current, maximum supply voltage (VDD,max), and effective inverter drive current (Ieff) for novel channel materials (InGaAs and Ge nMOS) are compared with those of Si nMOS at 27 °C vs. 101 °C. For novel channel materials, leakages from source-to-drain or band-to-band tunneling may become significant, while their T-dependence is much lower than in thermal leakage. This may have significant impact on the benchmarking analysis results, such as the increased VDD,max of novel channel materials at high T, which allows one to exploit the boosted Ieff in a wider range of supply voltage with leakage power still matching Si. This illustrates the importance of considering operating T's of actual circuits when assessing individual transistors, especially for novel channel materials.
A lab-on-a-chip system with integrated sample preparation and loop-mediated isothermal amplification for rapid and quantitative detection of Salmonella spp. in food samples (2015)

Foodborne disease is a major public health threat worldwide. Salmonellosis, an infectious disease caused by Salmonella spp., is one of the most common foodborne diseases. Isolation and identification of Salmonella by conventional bacterial culture or molecular-based methods are time consuming and usually take a few hours to days to complete. In response to the demand for rapid on line or on site detection of pathogens, in this study, we describe for the first time an eight-chamber lab-on-a-chip (LOC) system with integrated magnetic bead-based sample preparation and loop-mediated isothermal amplification (LAMP) for rapid and quantitative detection of Salmonella spp. in food samples. The whole diagnostic procedures including DNA isolation, isothermal amplification, and real-time detection were accomplished in a single chamber. Up to eight samples could be handled simultaneously and the system was capable to detect Salmonella at concentration of 50 cells per test within 40 min. The simple design, together with high level of integration, isothermal amplification, and quantitative analysis of multiple samples in short time, will greatly enhance the practical applicability of the LOC system for rapid on-site screening of Salmonella for applications in food safety control, environmental surveillance, and clinical diagnostics.
Properties and applications of Sn-doped single crystal thin film magneto-resistance elements (2006)

Using Sn-doped single crystal InSb thin films of 1.0-/spl mu/m thickness grown on GaAs substrates by molecular beam epitaxy, new magneto-resistance elements with very small temperature dependence were developed. Using these magneto-resistance elements, the contactless detection of the rotation speed of rotating gear teeth over a wide range of rotation speeds was studied. The output voltage from the magneto-resistance element was independent of the rotation speed of the gear, and exhibited good temperature stability and very small sine wave sine wave distortion, which allowed fine detection of the angular velocity of the rotations.
A simple constrained based adaptive null steering algorithm (2008)

This paper presents and investigates a simple constrained based adaptive null steering algorithm. In the environment where the desired signal is stronger or not significantly weaker than the interferences, nulling in the direction of interest becomes a problem for conventional zero tracking algorithm. With the proposed algorithm in this paper, the null positions can be selectively tracked one after another without affecting other null positions so that the interference signals can be rejected while the desired signal preserves. The main advantage of the proposed algorithm is its simplicity. When compared with conventional LMS based Linear Constrained Minimum Variance (LCMV) beamforming algorithm, the proposed method converges in a faster manner; and compared with QR-RLS based Minimum Variance Distortionless Response (MVDR) beamformer, the proposed algorithm has lower complexity. Furthermore, due to its cascade configuration, the steering vector error problem can be resolved by using the new method.
3D-Printed Microarray Patches for Transdermal Applications (2022)

The intradermal (ID) space has been actively explored as a means for drug delivery and diagnostics that is minimally invasive. Microneedles or microneedle patches or microarray patches (MAPs) are comprised of a series of micrometer-sized projections that can painlessly puncture the skin and access the epidermal/dermal layer. MAPs have failed to reach their full potential because many of these platforms rely on dated lithographic manufacturing processes or molding processes that are not easily scalable and hinder innovative designs of MAP geometries that can be achieved. The DeSimone Laboratory has recently developed a high-resolution continuous liquid interface production (CLIP) 3D printing technology. This 3D printer uses light and oxygen to enable a continuous, noncontact polymerization dead zone at the build surface, allowing for rapid production of MAPs with precise and tunable geometries. Using this tool, we are now able to produce new classes of lattice MAPs (L-MAPs) and dynamic MAPs (D-MAPs) that can deliver both solid state and liquid cargos and are also capable of sampling interstitial fluid. Herein, we will explore how additive manufacturing can revolutionize MAP development and open new doors for minimally invasive drug delivery and diagnostic platforms.
The essential oil of Rhus coriaria L. fruits (1993)

Abstract The steam volatile constituents of the oriental spice sumac, the dry fruits of Rhus coriaria L., were analysed by GC and GC–MS. During comparative studies with plant material from six Turkish provinces, more than 120 substances could be identified. Main constituents are terpene hydrocarbons (i.e. α‐pinene, β‐caryophyllene and cembrene), oxygenated terpenes (i.e. α‐terpineol, carvacrol and β‐caryophyllene alcohol) as well as farnesyl acetone, hexahydrofarnesylacetone and aliphatic aldehydes. In addition, the fatty acids of the seed fat have been analysed as their methyl esters.
MID-TERM RESULTS AFTER TREATMENT OF EXCENTRED DEGENERATION OF THE SHOULDER JOINT: HEMIARTHROPLASTY VERSUS INVERTED ARTHROPLASTY (2004)

Purpose: The purpose of this work was to compare outcome after hemiarthroplasty and after inverted arthroplasty for the treatment of excentred degeneration of the shoulder joint. Material and methods: This multicentric study inclued 136 patients, 110 women and 26 men, mean age 72.4 years (range 55 – 86 years) with 142 shoulder prostheses. Among the 142 shoulders operated on, 62 underwent Aequalis hemiarthroplasty and 80 inverted arthroplasty. The preoperative handicap was more severe in the hemiarthroplasty group (Constant score 26) than in the inverted arthroplasty group (Constant score 23). Results: Clinical and radiological follow-up data were collected at a mean follow-up of 44 months for the Aequalis prostheses and at 45 months for the inverted prostheses. There were seven failures requiring five revision procedures: three in the hemiarthroplasty group and four in the inverted arthroplasty group. The differences between the hemi- and inverted arthroplasty groups concerned the Constant score, which was significantly better in the inverted prostheses (65.5) than in the hemiarthroplasties (46.1), for all subscores. Active elevation was 138° for the inverted prostheses and 97° for the Aequalis prostheses (p Radiographically, there was one case with an anomalous humeral component (impaction) in the inverted group. For the inverted prostheses, there were three migrations that have not been revised to date and three partial screw loosenings. The main problem was the development of notches in the scapular column observed in 50% of the cases including 20% which reached the lower screw. For the Aequalis prostheses, the main problem was deterioration of the acromial vault observed in 50% of the cases leading to altered function. Discussion: Inverted prostheses provide clearly better mid-term results, even after five years. With hemiarthroplasty there is a long-term risk of vault wear. The long-term course of the inverted prostheses is worrisome due to the notches in the scapular column and the stress on the fixation. These prostheses should be used preferably for old persons with a deteriorated vault.
Vaccination of SARS-CoV-2-infected individuals expands a broad range of clonally diverse affinity-matured B cell lineages (2023)

Vaccination of SARS-CoV-2 convalescent individuals generates broad and potent antibody responses. Here, we isolate 459 spike-specific monoclonal antibodies (mAbs) from two individuals who were infected with the index variant of SARS-CoV-2 and later boosted with mRNA-1273. We characterize mAb genetic features by sequence assignments to the donors' personal immunoglobulin genotypes and assess antibody neutralizing activities against index SARS-CoV-2, Beta, Delta, and Omicron variants. The mAbs used a broad range of immunoglobulin heavy chain (IGH) V genes in the response to all sub-determinants of the spike examined, with similar characteristics observed in both donors. IGH repertoire sequencing and B cell lineage tracing at longitudinal time points reveals extensive evolution of SARS-CoV-2 spike-binding antibodies from acute infection until vaccination five months later. These results demonstrate that highly polyclonal repertoires of affinity-matured memory B cells are efficiently recalled by vaccination, providing a basis for the potent antibody responses observed in convalescent persons following vaccination.
Low Latency Driven Effective Capacity Analysis for Non-Orthogonal and Orthogonal Spectrum Access (2018)

In this paper, we theoretically investigate the performance of non-orthogonal and orthogonal spectrum access protocols (more generically known as NOMA) in supporting ultra-reliable low-latency communications (URLLC). The theory of effective capacity (EC) is adopted as a suitable delay-guaranteed capacity metric to flexibly represent the users' delay requirements. Then, the total EC difference between a downlink user-paired NOMA network and a downlink orthogonal multiple access (OMA) network is analytically studied. Exact closed-form expressions and the approximated closed-forms at high signal-to-noise ratios (SNRs) are derived for both networks and validated through simulation results. It is shown that for a user pair in which two users with the most distinct channel conditions are paired together, NOMA still achieves higher total EC (compared to OMA) in high SNR regime as the user group size becomes larger, although the EC performance of both NOMA and OMA reduces with the increase in group size. It is expected that the derived analytical framework can serve as a useful reference and practical guideline for designing favourable orthogonal and nonorthogonal spectrum access schemes in supporting low-latency services.
Front Cover: Formation and Surface Behavior of Pt and Pd Complexes with Ligand Systems Derived from Nitrile‐functionalized Ionic Liquids Studied by XPS (ChemPhysChem 2/2023) (2023)

The Front Cover shows substitution of volatile acetonitrile ligands by a nitrile-functionalized imidazolium cation. The formed cationic complex and the counter ions exhibit a specific preferential orientation at the ionic liquid/gas interface, which can be studied by angle-resolved XPS. More information can be found in the Research Article by Hans-Peter Steinrück and co-workers.
Precursor‐Directed Biosynthesis of 6‐Deoxyerythronolide B Analogs in Streptomycescoelicolor: Understanding Precursor Effects (2000)

A fermentation process employing precursor-directed biosynthesis is being developed for the manufacture of 6-deoxyerythronolide B (6-dEB) analogues. Through a plasmid-based system in Streptomyces coelicolor, 6-dEB synthesis is catalyzed by 6-dEB synthase (DEBS). 6-dEB synthesis is abolished by inactivation of the ketosynthase (KS) 1 domain of DEBS but can be restored by providing synthetic activated diketides. Because of its inherent catalytic flexibility, the KS1-deficient DEBS is capable of utilizing unnatural diketides to form various 13-substituted 6-dEBs. Here we characterize process variables associated with diketide feeding in shake-flask experiments. 13-R-6-dEB production was found to depend strongly on diketide feed concentrations, on the growth phase of cultures at feeding time, and on the R-group present in the diketide moiety. In all cases a major portion of the fed diketides was degraded by the cells.
Securing the Systems of the Future - Techniques for a Shifting Attack Space (2018)

Panel Overview Known security vulnerabilities across the computing stack have caused significant concern, even requiring extensive countermeasures and system patches to address. As an example, the Meltdown and Spectre attacks, which were disclosed in January 2018, exploit architectural and circuit vulnerabilities to allow a malicious process access to secrets stored in the memory of another running program. Although software-based patches were distributed, true hardware solutions are only available through replacement.