gebhart/patrepeval huggingface dataset:
A role for tumour necrosis factor‐α, Fas and Fas‐Ligand in marrow failure associated with myelodysplastic syndrome (1998)

Apoptosis of haemopoietic cells in the marrow of patients with myelodysplastic syndrome (MDS) has been suggested as a mechanism for peripheral cytopenias. We determined the expression of Fas (CD95), Fas-Ligand (Fas-L) and TNF-alpha factors known to be involved in apoptosis, in the marrow of 44 patients with MDS and characterized their functional relevance in in vitro assays of haemopoiesis. Multidimensional flow cytometry revealed phenotypically aberrant blasts as defined by orthogonal light scatter and CD45 expression in the marrow of 24/44 patients. Among those blasts Fas expression was increased on CD34-positive cells and on cells co-expressing HLA-DR. In addition, Fas-L was expressed on some CD34+ cells of MDS patients but was never detected on CD34+ cells in normal marrow. Fas and Fas-L mRNAs as well as mRNA for TNF-alpha, known to increase Fas expression in normal marrow, were up-regulated in patients with MDS. TNF-alpha protein and sTNF-R1 levels in marrow plasma were higher in MDS patients than in controls (P<0.002 and <0.003, respectively). However, results were dependent upon disease category: TNF-alpha levels were significantly higher in patients with refractory anaemia (RA) than in patients with RA with excess blasts (RAEB) or RAEB in transformation (RAEB-T) (P=0.043). Conversely, the proportion of Fas-L-positive cells was lowest in patients with RA (P=0.037). In marrow cultures, Fas-Ig, rhuTNFR:Fc or anti-TNF-alpha antibody, by blocking Fas or TNF mediated signals, respectively, significantly increased the numbers of haemopoietic colonies compared to untreated cells (P<0.001, P<0.003, P<0.001, respectively). These results show significant dysregulation in the expression of TNF-alpha, Fas and Fas-L in the marrow from MDS patients. Altered expression of these molecules appears to be of functional relevance in the dysregulation of haemopoiesis in MDS and may be amenable to therapeutic interventions.
First Principles Study of the Li[subscript 10]GeP[subscript 2]S[subscript 12] Lithium Super Ionic Conductor Material (2011)

The continued drive for high performance lithium batteries has imposed stricter requirements on the electrolyte materials. Solid electrolytes comprising lithium super ionic conductor materials exhibit good safety and stability and are promising to replace current organic liquid electrolytes. One major limitation in the application of Li-ion conductors is that their typical conductivity is less than 10[superscript −4] S/cm at room temperature. Recently, Kamaya et al. reported a new Li super ionic conductor Li[subscript 10]GeP[subscript 2]S[subscript 12] (LGPS), which has the highest conductivity ever achieved among solid lithium electrolytes of 12 mS/cm at room temperature (comparable conductivity with liquid electrolytes) and outstanding electrochemical performance in Li batteries.
Outer membranes of environmental isolates of Pseudomonas aeruginosa (1988)

The outer membrane composition of 30 environmental isolates of Pseudomonas aeruginosa was examined. Other than variations in the amounts of lipoprotein H2, there were no major differences in the outer membrane protein or lipopolysaccharide patterns when compared with those of previously studied clinical isolates.
Characterization of HLA DR13-restricted CD4+ T cell epitopes of hepatitis B core antigen associated with self-limited, acute hepatitis B (2002)

The HLA DR13 allele has been associated with a self-limited course of hepatitis B virus infection, possibly through the induction of a more vigorous hepatitis B core antigen (HBcAg) and/or hepatitis B e antigen-specific CD4(+) T cell response. HBcAg-specific CD4(+) T cell responses were investigated in three HLA DR13-positive subjects with self-limited, acute hepatitis B. HBcAg-specific, short-term T cell lines derived from these three subjects showed a dominant recognition of HBcAg peptides spanning aa 1-20 (P1), 11-30 (P2), 41-60 (P5), 111-131 (P12) and 141-160 (P15). In order to characterize these epitopes in more detail, CD4(+) T cell clones and cell lines were generated using HBcAg. Surprisingly, 11 of 12 T cell clones examined recognized P15; one recognized P10 (aa 91-111). Of four T cell lines, two recognized P15 and two recognized P5. By peptide mapping, the minimal epitope of P15 was located to residues (147)TVVRRRGRSP(156).
Polymeric Nanoparticle-Mediated GFAP-TIMP-1 Gene Delivery to Human Astrocytes (2018)

Purpose: The neuroprotective functions of astrocyte tissue inhibitor of metalloproteinases-1 (TIMP-1) during HIV-1-induced apoptosis are documented. However, astrocyte TIMP-1 levels decrease during chronic inflammation typical of HIV-associated neurocognitive disorders (HAND). We hypothesize that nanoparticle (NPs) mediated, astrocyte-targeted TIMP-1-gene delivery could restore TIMP-1 levels and serve as a HAND therapy. Optimally biocompatible polymeric NPs with validated in vivo reporter gene expression would serve as gene delivery vehicles. TIMP-1 overexpression may be restricted to astrocytes, using a glial fibrillary acidic protein (GFAP) promoter-driven gene expression, without adversely affecting the astrocyte structure and functions.\nMethods: We tested biocompatibility, dose-time kinetics, and in vivo gene delivery with arginine-modified polyethylenimine (PEI) analogs (AnPn) in vitro (primary human neural cells) and in vivo (mice) using a cytomegalovirus (CMV) promoter-driven luciferase plasmid (pLuc). Truncated and full-length GFAP promoter-driven plasmids encoding luciferase-reporter (gfa-Luc) and TIMP-1 (gfa-TIMP-1) were sub-cloned. Astrocytes transfected with AnPn-delivered gfa-Luc and gfa-TIMP-1 were evaluated for morphological changes, inflammatory biomarker profiles, and glutamate clearance to evaluate if critical astrocyte functions are affected.\nResults: Select PEI analogs (AnPn) led to robust and sustained reporter gene expression in astrocytes. Successful in vivo reporter gene delivery to the brain was confirmed by luminescence assay and immunohistochemistry. Truncated GFAP promoters led to a detectable gene expression in astrocytes compared to full-length GFAP promoters, which was regulated by inflammatory stimuli. Lastly, gfa-TIMP-1 plasmids were successfully delivered using AnPn to astrocytes in the presence and absence of HIV-relevant stimuli. Immunocytochemistry, and proinflammatory biomarker profiles of transfected astrocytes were analyzed to delineate NP-specific versus TIMP-1-specific changes.\nConclusions: These studies confirmed successful polymeric NPs-mediated reporter gene delivery in vitro and in vivo. Subsequently, NPs-mediated GFAP promoter-driven reporter and TIMP-1 gene expression was demonstrated in vitro. These findings serve as proof-of-concept towards testing therapeutic TIMP-1 gene delivery and demand future investigations geared towards clinical translations for HAND treatment.
The Heterogeneous Kinetics of the Reactions ClONO2 + HX/ice (X = Br, I), BrONO2 + HI/ice and the Reactivity of the Interhalogens BrCl, ICl and IBr with HX/ice (X = Cl, Br, I) in the Temperature Range 180 to 205 K (2000)

The kinetics and mechanism of the title heterogeneous halogen exchange reactions of potential atmospheric importance have been studied under molecular flow conditions in a FEP Teflon-coated Knudsen flow reactor on HX (X = Cl, Br, I) - doped ice condensed from the vapor phase under conditions of several formal monolayers of HX coverage at approximately 200 K. In addition, the halogen exchange reactions involving the expected primary reaction products BrCl, ICl and IBr of the above mixed anhydrides with HX-doped ice have been studied at 200 K as well. The uptake coefficient γ for the heterogeneous reaction ClONO
Ultra-thin Near-infrared Camera via Single Flat lens for Wide-angle Imaging (2020)

By utilizing a single multi-level diffractive lens coupled along with a conventional monochrome image sensor, we demonstrate a ~1mm thick near-infrared camera with a Field Of View up to 50° and on-axis focusing efficiency &gt; 90%.
Design and Optimization of a Biomass Proximity Sensor (2009)

A biomass population sensor can benefit producers, seed companies, and researchers. The primary advantage of this sensor is the ability to generate better site-specific population density maps. The primary objective of this study was to develop a capacitance-based biomass proximity sensor with the performance characteristics necessary to detect the presence of biomass (e.g., corn stalks). The detection and quantification of corn stalks under harvest conditions was chosen as an example application of this technology. In this study, a non-intrusive, capacitive, single-sided biomass proximity sensor was developed, and the suitability of this sensor to biomass population quantification was evaluated. A number of capacitive sensor patterns were simulated using the finite element method, and then the patterns were fabricated and tested in the laboratory. The design, modeling, and laboratory testing resulted in a high-sensitivity, low-noise sensing system that utilized a capacitive sensor, Wien bridge oscillator, phase-locked loop, and operational amplifier that could detect stalk presence and transform sensor capacitance change into a change in electrical potential. Sensor operating parameters were optimized to detect corn stalks in this application. This sensor system was then evaluated under field harvest conditions. The signal-to-noise ratio of this system was greater than 10 in both laboratory and proof-of-concept field tests. When compared to hand counts obtained before harvest, the sensor count error was less than 5% for five of the six rows harvested and less than 2% averaged over the six rows harvested. Future work will involve comprehensive field evaluation of the moisture-based biomass proximity sensor to quantify corn stalk population.
Discovery of natural‐derived Mpro inhibitors as therapeutic candidates for COVID‐19: Structure‐based pharmacophore screening combined with QSAR analysis (2023)

The main protease (M<sup>pro</sup> ) is an essential enzyme for the life cycle of SARS-CoV-2 and a validated target for treatment of COVID-19 infection. Structure-based pharmacophore modeling combined with QSAR calculations were employed to identify new chemical scaffolds of M<sup>pro</sup> inhibitors from natural products repository. Hundreds of pharmacophore models were manually built from their corresponding X-ray crystallographic structures. A pharmacophore model that was validated by receiver operating characteristic (ROC) curve analysis and selected using the statistically optimum QSAR equation was implemented as a 3D-search tool to mine AnalytiCon Discovery database of natural products. Captured hits that showed the highest predicted inhibitory activities were bioassayed. Three active M<sup>pro</sup> inhibitors (pseurotin A, lactupicrin, and alpinetin) were successfully identified with IC<sub>50</sub> values in low micromolar range.
Prospective evaluation of cell kinetics, yields and donor experiences during a single large‐volume apheresis versus two smaller volume consecutive day collections of allogeneic peripheral blood stem cells (2003)

We report cell kinetics, yields and donation experiences of 20 demographically matched allogeneic peripheral blood stem cell (PBSC) donors who were prospectively assigned to undergo either a single 25 l or two consecutive daily 15 l (15 l x 2) apheresis procedures. Procedures were performed using prophylactic intravenous calcium administration after standard granulocyte colony-stimulating factor (GCSF) mobilization (10 microg/kg/d). Central line placements (two each), initial CD34 cell counts (0.077 vs 0.078 x 10(9)/l) and yields (7.9 vs 8.1 x 10(8) CD34 cells) were similar in the two groups; however, 25 l donors spent significantly less time both in the clinic (7.5 vs 10.8 h) and with central venous catheters in place (8.5 vs 29.5 h) than 15 l x 2 donors. End-procedure platelet counts were below 100 x 10(9)/l in one out of 10 25 l donors versus five out of 10 in 15 l x 2 donors (41%vs 53% mean decrease in platelet counts, P = 0.02). PBSC collection efficiency increased by 37% after 15 l of the 25-l volume had been processed, compared with no significant change during 15 l x 2 procedures. Results similar to these prospective findings were also observed in CD34 yields, symptoms and platelet counts in additional 25 l and 15 l procedures performed during the same period and evaluated retrospectively. This study indicates that a single 25-l apheresis procedure results in similar yields and symptoms, but less donor thrombocytopenia and inconvenience than two consecutive daily 15-l procedures.
Biliary infection after liver transplantation (2009)

Aims: Biliary complications after liver transplantation are observed in approximately 20 percent of recipients and cholangitis in addition may complicate biliary leckage or stenosis. In this prospective study, the rate of bacterial and fungal infections in bile samples and their association with clinical parameters was evaluated.
Storage system control for correct operation of fault protections of single phase load in grids dominated by inverters (2015)

The diffusion of grids generated by power electronic converters in which no rotating generators are present is growing day by day. In these grids, in case of fault, the maximum current is limited by the control of the converter. For this reason, traditional protections based on high current detection could fail. The risk of failure in fault detection is higher in single phase grids. In order to solve this problem different solutions have been proposed in literature. In this paper, the possibility of using a storage system connected to the network by means of an auxiliary power converter is investigated. In order to reduce the cost of the solution an half bridge single phase converter is used and a control strategy is presented. The results obtained by means of numerical simulations prove the effectiveness of the proposed solution.
Estimation of Direct Causal Effects (2006)

Many common problems in epidemiologic and clinical research involve estimating the effect of an exposure on an outcome while blocking the exposure's effect on an intermediate variable. Effects of this kind are termed direct effects. Estimation of direct effects is typically the goal of research aimed at understanding mechanistic pathways by which an exposure acts to cause or prevent disease, as well as in many other settings. Although multivariable regression is commonly used to estimate direct effects, this approach requires assumptions beyond those required for the estimation of total causal effects. In addition, when the exposure and intermediate variables interact to cause disease, multivariable regression estimates a particular type of direct effect-the effect of an exposure on an outcome when the intermediate is fixed at a specified level. Using the counterfactual framework, we distinguish this definition of a direct effect (controlled direct effect) from an alternative definition, in which the effect of the exposure on the intermediate is blocked, but the intermediate is otherwise allowed to vary as it would in the absence of exposure (natural direct effect). We illustrate the difference between controlled and natural direct effects using several examples. We present an estimation approach for natural direct effects that can be implemented using standard statistical software, and we review the assumptions underlying our approach (which are less restrictive than those proposed by previous authors).
Engineering Soybeans for Food and Health (2003)

Growth in consumption of soy foods is restricted by negative flavor associations of soybean products and a limitation in the range of possible food and beverage applications resulting from the constraints of soybean storage protein properties. Soybean biotechnology is addressing these issues and creating even healthier soy-based foods.
Pharmacokinetics of Sulindac in ESRD (1988)

Letters| December 09 2008 Pharmacokinetics of Sulindac in ESRD Subject Area: Nephrology Charles J. Diskin; Charles J. Diskin aOpelika Nephrology Referral Center, Inc., Opelika, Ala.; Search for other works by this author on: This Site PubMed Google Scholar W. Ravis; W. Ravis bAuburn University, Auburn, Ala., USA Search for other works by this author on: This Site PubMed Google Scholar K.D. Campagna; K.D. Campagna bAuburn University, Auburn, Ala., USA Search for other works by this author on: This Site PubMed Google Scholar C.R. Clark C.R. Clark bAuburn University, Auburn, Ala., USA Search for other works by this author on: This Site PubMed Google Scholar Nephron (1988) 50 (4): 397. https://doi.org/10.1159/000185216 Article history Published Online: December 09 2008 Content Tools Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation Charles J. Diskin, W. Ravis, K.D. Campagna, C.R. Clark; Pharmacokinetics of Sulindac in ESRD. Nephron 1 April 1988; 50 (4): 397. https://doi.org/10.1159/000185216 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsNephron Search Advanced Search This content is only available via PDF. 1988Copyright / Drug Dosage / DisclaimerCopyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Article PDF first page preview Close Modal You do not currently have access to this content.
Heterogeneity of IgGs: Role of Production, Processing, and Storage on Structure and Function (2014)

Detailed monoclonal antibody (mAb) characterization tools have enabled the discovery of structural variations, including many that compromise functionality or have other undesired properties. Size, charge, glycosylation, and disulfide bonding variants; oxidized amino acid residues; and polypeptide chain truncations, extensions, and cleavage points have been identified. Product quality attributes, including detection techniques, published knowledge about the process origins, and quality impacts of these variants are summarized.
Estimating Rigid Transformation With Correlated Observations (2015)

The problem of finding the rotation and the translation between two sets of corresponded points is known as the rigid transformation estimation problem. It plays a crucial role in many robotic applications such as “simultaneous localization and mapping” (SLAM), surface reconstruction, and inertial sensor calibration. The most widely used solution to this problem is based on performing the singular value decomposition (SVD) over a derived data matrix. A drawback of the SVD method is that it is a least-squares method and thus may fail to take into account the anisotropic and/or correlated noises, which often present in practical applications. A natural variation is to add a matrix weight to the least-squares problem to balance the estimation errors in different measurement directions. However, it becomes difficult to write down a closed form solution in this setup. In this paper, an efficient algorithm is presented to estimate the rigid transformation with correlated observations. The effectiveness of the proposed method is experimentally demonstrated on two robotic applications, namely the point set registration and the inertial sensor localization.
Supplementary Figure 1 from GA201 (RG7160): A Novel, Humanized, Glycoengineered Anti-EGFR Antibody with Enhanced ADCC and Superior <i>In Vivo</i> Efficacy Compared with Cetuximab (2023)

&lt;p&gt;PDF file - 307K, ADCC activity of GA201 using PMBCs as effector cells at E:T ratios of 1:1, 5:1 (24 hour LDH release measured) and 10:1 (4 hour LDH release measured). Multiple ADCC experiments were performed using a variety of different effector:target cell ratios (E:T). Figure 3 in the manuscript displays the results of ADDC assays using NK-92 cells as effectors at an E:T ratio of 3:1 and using human peripheral blood mononuclear cells (PBMCs) as effectors at an E:T ratio of 25:1. These E:T ratios represent the optimal experimental conditions that generated robust ADCC signal read outs. Supplementary Fig. S1 shows additional ADCC results for the MKN45 cell line using human PBMCs as effector cells at an E:T ratios of 1:1, 5:1 and 10:1. The data shows that under all the tested conditions GA201 exhibited superior cell killing compared with cetuximab. The overall ADCC activity with both antibodies using PMBCs at an E:T ratio of 1:1 was substantially lower than at 5:1 or 10:1.&lt;/p&gt;
Influence of pulse amplitude and rise time on field-induced domain wall propagation in Ni80Fe20 nanowires (2008)

We have studied the field-induced propagation of domain walls (DWs) in magnetic nanowires by time-resolved magneto-optical Kerr microscopy. Magnetic field pulses with variable field amplitude lead to a DW velocity up to 500 m/s in 750 nm wide Ni80Fe20 nanowires, exceeding many other previous time-resolved optical measurements. Experiments show a linear relation between DW velocity and magnetic field beyond the Walker breakdown field, in agreement with micromagnetic simulations. In these simulations, the oscillation of DW type between vortex and transverse type, beyond Walker breakdown, is visualized. Furthermore, we experimentally show that the magnetic field pulse rise time is an important parameter for characterizing the DW velocity. Different pulse rise times lead to different injected DW types and DW velocities. These transient effects are especially important in possible future devices, where an ultrafast dynamical response of the DW is desired.
Integrated atomistic process and device simulation of decananometre MOSFETs (2003)

In this paper we present a methodology for the integrated atomistic process and device simulation of decananometre MOSFETs. The atomistic process simulations were carried out using the kinetic Monte Carlo process simulator DADOS, which is now integrated into the Synopsys 3D process and device simulation suite Taurus. The device simulations were performed using the Glasgow 3D statistical atomistic simulator, which incorporates density gradient quantum corrections. The overall methodology is illustrated in the atomistic process and device simulation of a well behaved 35 nm physical gate length MOSFET reported by Toshiba.