gebhart/patrepeval huggingface dataset:
A Formal Modeling of Managed Object Behaviour with Dynamic Temporal Properties (2000)

Recommendations of ITU-T and ISO stipulate the managerial abstraction of static and dynamic characteristics of network elements, management functions as well as management communication protocol. The current recommendations provide the formal mechanism for the structural parts of managed objects such as managed object class and attributes. But the current description method does not provide the formal mechanism for the behavioral characteristics of managed objects in clear manner but in natural language form, the complete specification of managed objects is not fully described. Also, the behaviour of managed objects is affected by their temporal and active properties. While the temporal properties representing periodic or repetitive internals are to describe managed objects behaviour in rather strict way, it will be more powerful if more dynamic temporal properties determined by external conditions are added to managed objects. In this paper, we added dynamic features to scheduling managed objects, and described, in GDMO, scheduling managed objects that support dynamic features. We also described behaviour of managed objects in newly defined BDL that has dynamic temporal properties. This paper showed that dynamic temporal managed objects provide a systematic and formal method in agent management function model.
Supplementary Figure S2 from PTEN Regulates Glutamine Flux to Pyrimidine Synthesis and Sensitivity to Dihydroorotate Dehydrogenase Inhibition (2023)

&lt;p&gt;Supplementary Figure S2. Pyrimidine synthesis is upregulated in Pten-/- MEFs.&lt;/p&gt;
ChemInform Abstract: Emerging Organosulfonium Electrophiles as Unique Reagents for Carbon—Sulfur Bond Formation: Prospects in Synthetic Chemistry of Organosulfur Compounds (2016)

Abstract Review: [54 refs.
New hardware and workflows for semi-automated correlative cryo-fluorescence and cryo-electron microscopy/tomography (2016)

Correlative light and electron microscopy allows features of interest defined by fluorescence signals to be located in an electron micrograph of the same sample. Rare dynamic events or specific objects can be identified, targeted and imaged by electron microscopy or tomography. To combine it with structural studies using cryo-electron microscopy or tomography, fluorescence microscopy must be performed while maintaining the specimen vitrified at liquid-nitrogen temperatures and in a dry environment during imaging and transfer. Here we present instrumentation, software and an experimental workflow that improves the ease of use, throughput and performance of correlated cryo-fluorescence and cryo-electron microscopy. The new cryo-stage incorporates a specially modified high-numerical aperture objective lens and provides a stable and clean imaging environment. It is combined with a transfer shuttle for contamination-free loading of the specimen. Optimized microscope control software allows automated acquisition of the entire specimen area by cryo-fluorescence microscopy. The software also facilitates direct transfer of the fluorescence image and associated coordinates to the cryo-electron microscope for subsequent fluorescence-guided automated imaging. Here we describe these technological developments and present a detailed workflow, which we applied for automated cryo-electron microscopy and tomography of various specimens.
A “Catalyst Switch” Strategy for the Sequential Metal-Free Polymerization of Epoxides and Cyclic Esters/Carbonate (2014)

A “catalyst switch” strategy was used to synthesize well-defined polyether–polyester/polycarbonate block copolymers. Epoxides (ethylene oxide and/or 1,2-butylene oxide) were first polymerized from a monoalcohol in the presence of a strong phosphazene base promoter (t-BuP4). Then an excess of diphenyl phosphate (DPP) was introduced, followed by the addition and polymerization of a cyclic ester (ε-caprolactone or δ-valerolactone) or a cyclic carbonate (trimethylene carbonate), where DPP acted as both the neutralizer of phosphazenium alkoxide (polyether chain end) and the activator of cyclic ester/carbonate. This work has provided a one-pot sequential polymerization method for the metal-free synthesis of block copolymers from monomers which are suited for different types of organic catalysts.
Flow Characteristics of a Pilot-Scale High Temperature, Short Time Pasteurizer (2004)

In this study, we present a method for determining the fastest moving particle (FMP) and residence time distribution (RTD) in a pilot-scale high temperature, short time (HTST) pasteurizer to ensure that laboratory or pilot-scale HTST apparatus meets the Pasteurized Milk Ordinance standards for pasteurization of milk and can be used for obtaining thermal inactivation data. The overall dimensions of the plate in the pasteurizer were 75 x 115 mm, with a thickness of 0.5 mm and effective diameter of 3.0 mm. The pasteurizer was equipped with nominal 21.5- and 52.2-s hold tubes, and flow capacity was variable from 0 to 20 L/h. Tracer studies were used to determine FMP times and RTD data to establish flow characteristics. Using brine milk as tracer, the FMP time for the short holding section was 18.6 s and for the long holding section was 36 s at 72 degrees C, compared with the nominal times of 21.5 and 52.2 s, respectively. The RTD study indicates that the short hold section was 45% back mixed and 55% plug flow for whole milk at 72 degrees C. The long hold section was 91% plug and 9% back mixed for whole milk at 72 degrees C. This study demonstrates that continuous laboratory and pilot-scale pasteurizers may be used to study inactivation of microorganisms only if the flow conditions in the holding tube are established for comparison with commercial HTST systems.
Novel Bioactive and Therapeutic Dental Polymeric Materials to Inhibit Periodontal Pathogens and Biofilms (2019)

Periodontitis is a common infectious disease characterized by loss of tooth-supporting structures, which eventually leads to tooth loss. The heavy burden of periodontal disease and its negative consequence on the patient's quality of life indicate a strong need for developing effective therapies. According to the World Health Organization, 10⁻15% of the global population suffers from severe periodontitis. Advances in understanding the etiology, epidemiology and microbiology of periodontal pocket flora have called for antibacterial therapeutic strategies for periodontitis treatment. Currently, antimicrobial strategies combining with polymer science have attracted tremendous interest in the last decade. This review focuses on the state of the art of antibacterial polymer application against periodontal pathogens and biofilms. The first part focuses on the different polymeric materials serving as antibacterial agents, drug carriers and periodontal barrier membranes to inhibit periodontal pathogens. The second part reviews cutting-edge research on the synthesis and evaluation of a new generation of bioactive dental polymers for Class-V restorations with therapeutic effects. They possess antibacterial, acid-reduction, protein-repellent, and remineralization capabilities. In addition, the antibacterial photodynamic therapy with polymeric materials against periodontal pathogens and biofilms is also briefly described in the third part. These novel bioactive and therapeutic polymeric materials and treatment methods have great potential to inhibit periodontitis and protect tooth structures.
CELL‐CYCLE ANALYSIS OF PERTURBED CELL POPULATIONS: COMPUTER SIMULATION OF SEQUENTIAL DNA DISTRIBUTIONS (1976)

A mathematical model is presented that permits simulation of a time sequence of DNA distributions with a single set of cell-cycle parameters. The method is particularly suited to the quantitative analysis of sets of sequential DNA distributions from perturbed cell populations. The model permits determination of the durations and associated dispersions of the phases of the cell cycle as well as the point in the cell cycle at which the perturbing agent exerts its effect. The mathematical details of the simulation technique are presented, and the technique is applied to the analysis of DNA distributions from perturbed cell populations. Three cell populations are modeled: CHO-line cells released from a block at the interface of the G1-and S-phases, 3T3 cells released from a G1-phase block produced by serum starvation, and S49 mouse lymphoma cells responding to a block in the G1-phage produced by N6,02'-dibutyryl adenosine 3':5'-cyclic monophosphate (Bt2cAMP).
COVID-19 awareness, knowledge and perception towards digital health in an urban multi-ethnic Asian population (2021)

This study aimed to determine COVID-19-related awareness, knowledge, impact and preparedness among elderly Asians; and to evaluate their acceptance towards digital health services amidst the pandemic. 523 participants (177 Malays, 171 Indians, 175 Chinese) were recruited and underwent standardised phone interview during Singapore's lockdown period (07 April till 01 June 2020). Multivariable logistic regression models were performed to evaluate the associations between demographic, socio-economic, lifestyle, and systemic factors, with COVID-19 awareness, knowledge, preparedness, well-being and digital health service acceptance. The average perception score on the seriousness of COVID-19 was 7.6 ± 2.4 (out of 10). 75.5% of participants were aware that COVID-19 carriers can be asymptomatic. Nearly all (≥ 90%) were aware of major prevention methods for COVID-19 (i.e. wearing of mask, social distancing). 66.2% felt prepared for the pandemic, and 86.8% felt confident with government's handling and measures. 78.4% felt their daily routine was impacted. 98.1% reported no prior experience in using digital health services, but 52.2% felt these services would be helpful to reduce non-essential contact. 77.8% were uncomfortable with artificial intelligence software interpreting their medical results. In multivariable analyses, Chinese participants felt less prepared, and more likely felt impacted by COVID-19. Older and lower income participants were less likely to use digital health services. In conclusion, we observed a high level of awareness and knowledge on COVID-19. However, acceptance towards digital health service was low. These findings are valuable for examining the effectiveness of COVID-19 communication in Singapore, and the remaining gaps in digital health adoption among elderly.
Electrolyte-Mediated Assembly of Charged Nanoparticles* (2020)

Solutions at high salt concentrations are used to crystallize or segregate charged colloids, including proteins and polyelectrolytes 898via a complex mechanism referred to as “salting-out.” Here, we combine small-angle X-ray scattering (SAXS), molecular dynamics (MD) simulations, and liquid-state theory to show that salting-out is a long-range interaction, which is controlled by electrolyte concentration and colloid charge density. As a model system, we analyze Au nanoparticles coated with noncomplementary DNA designed to prevent interparticle assembly via Watson–Crick hybridization. SAXS shows that these highly charged nanoparticles undergo “gas” to face-centered cubic (FCC) to “glass-like” transitions with increasing NaCl or CaCl2 concentration. MD simulations reveal that the crystallization is concomitant with interparticle interactions changing from purely repulsive to a “long-range potential well” condition. Liquid-state theory explains this attraction as a sum of cohesive and depletion forces that originate from the interelectrolyte ion and electrolyte–ion–nanoparticle positional correlations. Our work provides fundamental insights into the effect of ionic correlations in the salting-out mechanism and suggests new routes for the crystallization of colloids and proteins using concentrated salts.
A study on a dual-voltage self-clamped IGBT for automotive ignition applications (2002)

Self-clamped Inductive Switching (SCIS) energy capability is a critical parameter of device performance for IGBTs used in automotive ignition applications. In this paper, we studied a monolithic dual-voltage self-clamped IGBT to improve its SCIS energy capability. During an inductive turnoff process, the collector voltage of the proposed device is clamped first at a high level for a short period of time and then at a much lower level until the electromagnetic energy stored in the inductive coil is fully discharged. The concept has been verified and analyzed with extensive numerical device simulation. A monolithic prototype device has been designed and fabricated with a conventional eight-mask IGBT process. The experimental results are reported.
Hybrid and Electric Automotive Systems: Combined Electrical, Mechanical, and Fuel Cell Opportunities for Personal Transportation (2006)

Electric automobiles are clean, efficient, and powerful but are limited in range. Hybrid designs seek to enhance range through the use of liquid fuel. The energy, force, and power needs of a typical car are reviewed. The relationship to fuel cells as energy sources is discussed. Opportunities in the electrical arena, the mechanical arena, and the fuel cell arena are presented. Two commercial hybrid vehicle examples are presented. The convergence of developments in these areas are dominate hybrid designs as commercial production continues to ramp up.
Abstract PR008: Armed Myxoma virus demonstrates therapeutic activity in pre-clinical xenograft models (2021)

Abstract Oncolytic Viruses (OV) selectively replicate in and lyse tumor cells and provide stimulation to the immune system. This represents a promising therapeutic option for cancer patients that do not respond well to treatment with immune checkpoint inhibitors. Myxoma virus (MYXV) is a member of the Poxviridae family of double stranded DNA viruses. The natural host of MYXV is a subset of lagomorphs, but MYXV is able to infect cancer cell lines of humans and other species. The genome of MYXV is relatively large and is amenable to engineering for expression of transgenic proteins making it an excellent oncolytic virus for introduction of immunomodulatory proteins. The current work describes the oncolytic activity, transgene production capability, and in vivo activity of armed myxoma viruses in human cancer models. This abstract is also being presented as PO090. Citation Format: Lino E. Torres-Dominguez, Lina S. Franco, Mario Abrantes, Benjamin S. Walker, Zachary Tacner, Cassandra Kien, Anna K. Waters, Grant McFadden, Steven J. Potts, Leslie L. Sharp. Armed Myxoma virus demonstrates therapeutic activity in pre-clinical xenograft models [abstract]. In: Abstracts: AACR Virtual Special Conference: Tumor Immunology and Immunotherapy; 2020 Oct 19-20. Philadelphia (PA): AACR; Cancer Immunol Res 2021;9(2 Suppl):Abstract nr PR008.
Synthesis, In Vitro Activities of (2-Cyclopropoxyphenyl)piperidine Derivatives for &#945;1a and &#945;1d Adrenergic Receptor Inhibitors (2009)

An alpha 1a- and alpha 1d-adrenergic receptor (AR) selective antagonist may be a more efficacious treatment for BPH/LUTS patients and may have fewer side effects than the existing pharmaceuticals. A facile synthesis for a series of (2-cyclopropoxyphenyl)piperidine derivatives has been developed, in which aryl vinyl ether formation and subsequent cyclopropyl formation provide efficient access to key intermediate N-Boc-4-(2-cyclopropoxyphenyl)piperidine. The synthesized (2-cyclopropoxyphenyl)piperidine derivatives display high affinity and selectivity for alpha1a-AR and alpha1d-AR compared to alpha1b-AR and D2 receptor, Ki values for alpha1a-AR are 0.91 nM to 79.0 nM and alpha1d-AR are 2.0 nM to 57 nM; Ki values for alpha1b-AR are 107 nM to 839.8 nM and D2 receptor are 66.2 nM to 187.1 nM. The selectivity ratios of Ki(alpha1b)/Ki(alpha1a) are 11 to 155 fold, Ki(alpha1b)/Ki(alpha1d) are 6 to 171 fold, Ki(D2)/Ki(alpha1a) are 2 to 158 fold, and Ki(D2)/Ki(alpha1d) are 1.2 to 89 fold. Compound 17a shows improved stability in human liver microsome test (t1/2 = 18 minutes).
Engineered dCas9 with reduced toxicity in bacteria: implications for genetic circuit design (2018)

Large synthetic genetic circuits require the simultaneous expression of many regulators. Deactivated Cas9 (dCas9) can serve as a repressor by having a small guide RNA (sgRNA) direct it to bind a promoter. The programmability and specificity of RNA:DNA basepairing simplifies the generation of many orthogonal sgRNAs that, in theory, could serve as a large set of regulators in a circuit. However, dCas9 is toxic in many bacteria, thus limiting how high it can be expressed, and low concentrations are quickly sequestered by multiple sgRNAs. Here, we construct a non-toxic version of dCas9 by eliminating PAM (protospacer adjacent motif) binding with a R1335K mutation (dCas9*) and recovering DNA binding by fusing it to the PhlF repressor (dCas9*_PhlF). Both the 30 bp PhlF operator and 20 bp sgRNA binding site are required to repress a promoter. The larger region required for recognition mitigates toxicity in Escherichia coli, allowing up to 9600 ± 800 molecules of dCas9*_PhlF per cell before growth or morphology are impacted, as compared to 530 ± 40 molecules of dCas9. Further, PhlF multimerization leads to an increase in average cooperativity from n = 0.9 (dCas9) to 1.6 (dCas9*_PhlF). A set of 30 orthogonal sgRNA-promoter pairs are characterized as NOT gates; however, the simultaneous use of multiple sgRNAs leads to a monotonic decline in repression and after 15 are co-expressed the dynamic range is <10-fold. This work introduces a non-toxic variant of dCas9, critical for its use in applications in metabolic engineering and synthetic biology, and exposes a limitation in the number of regulators that can be used in one cell when they rely on a shared resource.
The Use Of Soft Acid Metal Salts Of Carboxylic Acids For Retention Of Hydrophilic Peptides In Reversed-Phase High Performance Liquid Chromatography (1981)

Abstract The effect of various soft acid cation acetate salts on the retention of the peptides Phe-(Gly)n, where n=1–4, and on phenylalanine, indicates a correlation between the softness character of the cation of the acetate salt used in the mobile phase and retention in reversed-phase high performance liquid chromatography. Inorganic soft acid cation salts did not exhibit the same increased retention properties. By using silver(I) valerate or silver(I) octanoate as ion-pairing reagents in the mobile phase for RP-HPLC, static retention of peptides was achieved.
A flexible role-based secure messaging service: exploiting IBE technology for privacy in health care (2004)

The management of private and confidential information is a major problem for dynamic organizations. Secure solutions are needed to exchange confidential documents, protect them against unauthorized accesses and cope with changes of people's roles and permissions. Traditional cryptographic systems and PKI show their limitations, in terms of flexibility and manageability. This paper describes an innovative technical solution in the area of secure messaging that exploits identifier-based encryption (IBE) technology. It illustrates the advantages against a similar approach based on traditional cryptography and PKI. It discusses a few open issues. Our main contribution is a practical solutions based on IBE technology. A secure messaging system based on IBE has been fully implemented and it is currently used in a trial with a UK health service organization.
Reconfigurable Intelligent Surfaces for Smart Cities: Research\n Challenges and Opportunities (2020)

The concept of Smart Cities has been introduced as a way to benefit from the\ndigitization of various ecosystems at a city level. To support this concept,\nfuture communication networks need to be carefully designed with respect to the\ncity infrastructure and utilization of resources. Recently, the idea of 'smart'\nenvironment, which takes advantage of the infrastructure for better performance\nof wireless networks, has been proposed. This idea is aligned with the recent\nadvances in design of reconfigurable intelligent surfaces (RISs), which are\nplanar structures with the capability to reflect impinging electromagnetic\nwaves toward preferred directions. Thus, RISs are expected to provide the\nnecessary flexibility for the design of the 'smart' communication environment,\nwhich can be optimally shaped to enable cost- and energy-efficient signal\ntransmissions where needed. Upon deployment of RISs, the ecosystem of the Smart\nCities would become even more controllable and adaptable, which would\nsubsequently ease the implementation of future communication networks in urban\nareas and boost the interconnection among private households and public\nservices. In this paper, we describe our vision of the application of RISs in\nfuture Smart Cities. In particular, the research challenges and opportunities\nare addressed. The contribution paves the road to a systematic design of\nRIS-assisted communication networks for Smart Cities in the years to come.\n
The effect of nitric oxide synthase inhibitor on form-deprivation myopia (1997)

The injection of L-NAME before occlusion of developing chick eyes leads to reversible modifications in retinal function and inhibits the development of form-deprivation myopia.
Electrospun Nanofiber-Based Thermite Textiles and their Reactive Properties (2012)

In this work, we present a first time fabrication of thermite-based nanofiber mats with a nitrocellulose composite energetic binder to create a new class of energetic 1D nanocomposite. The as prepared thermite based nanofibrous mats were characterized and tested for their burning behavior, and compared with the pure nitrocellulose and nanoaluminum incorporated nanofibers for their combustion performances. Thermite-based nanofibers show enhanced burning rates in combustion tests, which correlate to the mass loading of nanothermite relative to binder in nanofibers. The electrospinning method demonstrates the possibility of avoiding some of the problems associated with melt casting nanometalized propellants.