gebhart/patrepeval huggingface dataset:
Complex of Burkholderia cepacia lipase with transition state analogue of 1‐phenoxy‐2‐acetoxybutane (2001)

In a series of four racemic phenoxyalkyl-alkyl carbinols, 1-phenoxy-2-hydroxybutane (1) is enantioselectively acetylated by Burkholderia cepacia (formerly Pseudomonas cepacia) lipase with an E value > or = 200, whereas for the other three racemates E was found to be < or = 4. To explain the high preference of B. cepacia lipase for (R)-(+)-1, a precursor of its transition state analogue with a tetrahedral P-atom, (R(P),S(P))-O-(2R)-(1-phenoxybut-2-yl)methylphosphonic acid chloride was prepared and crystallized in complex with B. cepacia lipase. The X-ray structure of the complex was determined, allowing to compare the conformation of the inhibitor with results of molecular modelling.
Spectrally Resolved Nonlinear Optical Properties of Doped Versus Undoped Quasi-2D Semiconductor Nanocrystals: Copper and Silver Doping Provokes Strong Nonlinearity in Colloidal CdSe Nanoplatelets (2022)

Nonlinear optical processes are crucial for emerging applications including multiphoton-excited fluorescence microscopy and optical power limiting. Therefore, searching for materials of high multiphoton absorption cross sections is essential for the development of these techniques. We present synthesis of 4.5 monolayer CdSe nanoplatelets (NPLs) doped with silver and copper ions along with the evaluation of their two-photon absorption (TPA) and three-photon absorption (3PA) cross sections. Doping significantly increases the TPA cross section of each NPL sample, which reaches up to 1.33 10 7 GM for the most absorbing copper-doped ones. We also detected 1-2 orders of magnitude-enhanced 3PA cross sections for the doped NPLs in comparison with their undoped counterparts. As TPA and 3PA peaks appear, in the first and the second biological transmission windows, respectively, doped NPLs are promising candidates for multiphoton fluorescence microscopy as bioimaging agents. Moreover, the strong nonlinear response suggests application as active optoelectronic materials in optical sensors.
Experimental results on a scanned beam microstrip antenna array with a proximity coupled YBCO feed network (1993)

The design, fabrication, and measurement of a scanned beam microstrip antenna array with a high-temperature superconducting YBCO feed network are described. A novel geometry is introduced which integrates the antenna array into a window on the vacuum enclosure of the cryocooler for maximum thermal and RF efficiency. The model considered includes surface wave and mutual coupling effects, which are of importance in large array applications. Experimental results demonstrate broadband radiation with expected gain values for broadside and 45-degree scan angles.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Axial Distortion and Electronic Structure in the Macrocyclic Complexes, Diacido-S,S'-o-xylyl-2,3 pentanedionebis(mercaptoethylimine) nickel(II) (1966)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAxial Distortion and Electronic Structure in the Macrocyclic Complexes, Diacido-S,S'-o-xylyl-2,3 pentanedionebis(mercaptoethylimine) nickel(II)George R. Brubaker and Daryle H. BuschCite this: Inorg. Chem. 1966, 5, 12, 2114–2118Publication Date (Print):December 1, 1966Publication History Published online1 May 2002Published inissue 1 December 1966https://pubs.acs.org/doi/10.1021/ic50046a007https://doi.org/10.1021/ic50046a007research-articleACS PublicationsRequest reuse permissionsArticle Views79Altmetric-Citations40LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Ultralow Limit Detection of Soluble HER2 Biomarker in Serum with a Fiber-Optic Ball-Tip Resonator Assisted by a Tilted FBG (2022)

An optical-fiber biosensor has been developed for the detection of the breast cancer biomarker soluble human epidermal growth factor receptor-2 (sHER2). The sensor was fabricated by combining a tilted fiber Bragg grating (TFBG) with a ball resonator, allowing us to achieve an excellent sensitivity compared to other optical-fiber-based sensors. The sensor exhibits a resonance comb excited by the TFBG and the spectral profile of the ball resonator. The detection of sHER2 at extremely low concentrations was carried out by tracking the amplitude change of selected resonances. The therapeutic anti-HER2 monoclonal antibody Trastuzumab has been used to functionalize the biosensor with silane surface chemistry. The sensor features a sensitivity of 4034 dB/RIU with a limit of detection (LoD) in buffer and in a 1/10 diluted serum of 151.5 ag/mL and 3.7 pg/mL, respectively. At relatively high protein concentrations (64 ng/mL) binding to sHER (7.36 dB) as compared to control proteins (below 0.7 dB) attested the high specificity of sHER2 detection.
RF Design, Power Handling, and Hot Switching of Waveguide Water-Based Absorptive Switches (2009)

<para xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> This paper presents the first complete water-based waveguide absorptive switch from 25–40 GHz integrated with commercially available micropumps. The design exploits the absorptive properties of water in the microwave and millimeter-wave bands along with innovative techniques to achieve an optimized performance in both switching states. Besides its static RF performance, the hot-switching response is also experimentally characterized. Successful hot-switching measurements are presented for power levels of up to 32 and 0.16 W for circulating and noncirculating water, respectively. This is achieved with a circulation rate of only <formula formulatype="inline"><tex Notation="TeX">${\sim} {\hbox{20 mL}}/{\hbox{min}}$</tex></formula>. We also show that this power handling can readily reach 125 and 1250 W if the circulation rate is increased to 30 and 300 mL/min, respectively. In addition, the dynamic scattering matrix under hot-switching conditions is also measured and compared to the cold-switching scattering matrix. Furthermore, critical temperature effects are also studied. In particular, contrary to common wisdom, we show that increased water temperature can result in improved RF isolation with the appropriate waveguide-switching design. </para>
Constitutive Activation of Signal Transducers and Activators of Transcription Predicts Vorinostat Resistance in Cutaneous T-Cell Lymphoma (2008)

Vorinostat is a histone deacetylase inhibitor that induces differentiation, growth arrest, and/or apoptosis of malignant cells both in vitro and in vivo and has shown clinical responses in approximately 30% of patients with advanced mycosis fungoides and Sézary syndrome cutaneous T-cell lymphoma (CTCL). The purpose of this study was to identify biomarkers predictive of vorinostat response in CTCL using preclinical model systems and to assess these biomarkers in clinical samples. The signal transducer and activator of transcription (STAT) signaling pathway was evaluated. The data indicate that persistent activation of STAT1, STAT3, and STAT5 correlate with resistance to vorinostat in lymphoma cell lines. Simultaneous treatment with a pan-Janus-activated kinase inhibitor resulted in synergistic antiproliferative effect and down-regulation of the expression of several antiapoptotic genes. Immunohistochemical analysis of STAT1 and phosphorylated tyrosine STAT3 (pSTAT3) in skin biopsies obtained from CTCL patients enrolled in the vorinostat phase IIb trial showed that nuclear accumulation of STAT1 and high levels of nuclear pSTAT3 in malignant T cells correlate with a lack of clinical response. These results suggest that deregulation of STAT activity plays a role in vorinostat resistance in CTCL, and strategies that block this pathway may improve vorinostat response. Furthermore, these findings may be of prognostic value in predicting the response of CTCL patients to vorinostat.
Measuring the field from the hard bias in spin valves (2005)

Summary form only given. The complete presentation was not made available for publication as part of the conference proceedings.
Off-Resonance Behaviour of RARE and TrueFISP in Imaging of Hyperpolarized 13C (2007)

Imaging of hyperpolarized 13C has recently gained interest for metabolic imaging. In comparison with 1H imaging, the signal of hyperpolarized substances cannot build up a steady state during an imaging sequence as it decays irreversibly to its thermal equilibrium value. Therefore, TrueFISP (FIESTA, balanced SSFP, b-FFE) and single-shot RARE (TSE, FSE) show comparable signal behaviour for on-resonance signal. However, TrueFISP images of hyperpolarized substances suffer in general from off-resonance signal in contrast to RARE. We demonstrate that due to off-resonance effects, TrueFISP has no advantage in comparison with RARE when hyperpolarized substances are imaged.
The Transverse Transmission/Reflection Method: An Experimental/Numerical Prism-Coupling-Based Approach for Leaky Mode Characterization in Planar Waveguides (2008)

A prism-coupler-based experimental/numerical method is presented to characterize leaky waveguide modes from the transmitted/reflected powers in the transverse direction. The loss is obtained without multi-site longitudinal measurements as in conventional methods [1–2].
CD28 Costimulatory Domain–Targeted Mutations Enhance Chimeric Antigen Receptor T-cell Function (2020)

An obstacle to the development of chimeric antigen receptor (CAR) T cells is the limited understanding of CAR T-cell biology and the mechanisms behind their antitumor activity. We and others have shown that CARs with a CD28 costimulatory domain drive high T-cell activation, which leads to exhaustion and shortened persistence. This work led us to hypothesize that by incorporating null mutations of CD28 subdomains (YMNM, PRRP, or PYAP), we could optimize CAR T-cell costimulation and enhance function. <i>In vivo</i>, we found that mice given CAR T cells with only a PYAP CD28 endodomain had a significant survival advantage, with 100% of mice alive after 62 days compared with 50% for mice with an unmutated endodomain. We observed that mutant CAR T cells remained more sensitive to antigen after <i>ex vivo</i> antigen and PD-L1 stimulation, as demonstrated by increased cytokine production. The mutant CAR T cells also had a reduction of exhaustion-related transcription factors and genes such as <i>Nfatc1, Nr42a</i>, and <i>Pdcd1</i> Our results demonstrated that CAR T cells with a mutant CD28 endodomain have better survival and function. This work allows for the development of enhanced CAR T-cell therapies by optimizing CAR T-cell costimulation.
Blood Accessibility to Fibrin in Venous Thrombosis is Thrombus Age-Dependent and Predicts Fibrinolytic Efficacy: An In Vivo Fibrin Molecular Imaging Study (2015)

Fibrinolytic therapy of venous thromboembolism (VTE) is increasingly utilized, yet limited knowledge is available regarding in vivo mechanisms that govern fibrinolytic efficacy. In particular, it is unknown how age-dependent thrombus organization limits direct blood contact with fibrin, the target of blood-based fibrinolytic agents. Utilizing high-resolution in vivo optical molecular imaging with FTP11, a near-infrared fluorescence (NIRF) fibrin-specific reporter, here we investigated the in vivo interrelationships of blood accessibility to fibrin, thrombus age, thrombus neoendothelialization, and fibrinolysis in murine venous thrombosis (VT). In both stasis VT and non-stasis VT, NIRF microscopy showed that FTP11 fibrin binding was thrombus age-dependent. FTP11 localized to the luminal surface of early-stage VT, but only minimally to subacute VT (p<0.001). Transmission electron microscopy of early stage VT revealed direct blood cell contact with luminal fibrin-rich surfaces. In contrast, subacute VT exhibited an encasing CD31+ neoendothelial layer that limited blood cell contact with thrombus fibrin in both VT models. Next we developed a theranostic strategy to predict fibrinolytic efficacy based on the in vivo fibrin accessibility to blood NIRF signal. Mice with variably aged VT underwent FTP11 injection and intravital microscopy (IVM), followed by tissue plasminogen activator infusion to induce VT fibrinolysis. Fibrin molecular IVM revealed that early stage VT, but not subacute VT, bound FTP11 (p<0.05), and experienced higher rates of fibrinolysis and total fibrinolysis (p<0.05 vs. subacute VT). Before fibrinolysis, the baseline FTP11 NIRF signal predicted the net fibrinolysis at 60 minutes (p<0.001). Taken together, these data provide novel insights into the temporal evolution of VT and its susceptibility to therapeutic fibrinolysis. Fibrin molecular imaging may provide a theranostic strategy to identify venous thrombi amenable to fibrinolytic therapies.
Molecular profiling reveals a hypoxia signature in breast implant-associated anaplastic large cell lymphoma (2020)

Breast implant-associated anaplastic large cell lymphoma (BIA-ALCL) is a recently characterized T-cell malignancy that has raised significant patient safety concerns and led to worldwide impact on the implants used and clinical management of patients undergoing reconstructive or cosmetic breast surgery. Molecular signatures distinguishing BIA-ALCL from other ALCLs have not been fully elucidated and classification of BIA-ALCL as a WHO entity remains provisional. We performed RNA sequencing and gene set enrichment analysis comparing BIA-ALCLs to non-BIA-ALCLs and identified dramatic upregulation of hypoxia signaling genes including the hypoxia-associated biomarker CA9 (carbonic anyhydrase-9). Immunohistochemistry validated CA9 expression in all BIA-ALCLs, with only minimal expression in non-BIA-ALCLs. Growth induction in BIA-ALCL-derived cell lines cultured under hypoxic conditions was proportional to up-regulation of CA9 expression, and RNA sequencing demonstrated induction of the same gene signature observed in BIA-ALCL tissue samples compared to non-BIA-ALCLs. CA9 silencing blocked hypoxia-induced BIA-ALCL cell growth and cell cycle-associated gene expression, whereas CA9 overexpression in BIA-ALCL cells promoted growth in a xenograft mouse model. Furthermore, CA9 was secreted into BIA-ALCL cell line supernatants and was markedly elevated in human BIA-ALCL seroma samples. Finally, serum CA9 concentrations in mice bearing BIA-ALCL xenografts were significantly elevated compared to control serum. Together, these findings characterize BIA-ALCL as a hypoxia-associated neoplasm, likely attributable to the unique microenvironment in which it arises. These data support classification of BIA-ALCL as a distinct entity and uncover opportunities for investigating hypoxia-related proteins such as CA9 as novel biomarkers and therapeutic targets in this disease.
The biologic significance of alloreactivity. The ontogeny of T-cell sets specific for alloantigens or modified self antigens (1978)

We have analyzed the cellular basis of T-cell reactivity against lymphocytes expressing major histocompatibility complex (MHC) products that are foreign by virtue of polymorphism (alloantigens) or because of modification by chemicals or viruses. We find that early in ontogeny, prekiller activity against both trinitrophenyl (TNP)-coupled autologous MHC products and allogeneic MHC products resides in the same (Ly123(+)) T-cell pool; later in ontogeny alloreactivity is invested in Ly23 cells which, when activated, lyse TNP-coupled autologous cells as well as appropriate allogeneic target cells. We demonstrate that stimulation of Ly123(+) T cells in vitro by autologous cells coated with chemically-inactivated Sendai virus results in the formation of Ly23(+) cytolytic T lymphocytes (CTL) that specifically lyse both virus modified autologous target cells and unmodified allogeneic target cells. These results suggest the following model to account for the presence of large numbers of alloreactive T-cell clones in adult animals: continuous stimulation of Ly123 cells by autologous MHC antigens associated with foreign materials such as a virus results in the formation of Ly23 memory progeny carrying receptors that recognize MHC products that are foreign due to genetic polymorphism (alloantigens). In general, these studies indicate that alloaggression (as manifest by Ly23 cells in the CTL response) reflects a high degree of cross stimulation between physiologically relevant antigens, e.g., viral determinants associated with self MHC products, and biologically irrelevant allelic variants of the MHC.
Mucins as biomarkers in cancer (2013)

Current diagnostic and prognostic biomarker assays for the detection of cancer-associated mucins and their carbohydrate modifications are discussed in this chapter. The classical CA15-3 and CA125 assays, as well as new glycoprofiling strategies, improve our ability to distinguish between benign and malignant disease.
A simple and scalable method for preparing low-defect ZIF-8 tubular membranes (2013)

Substrate modification by an ultrathin ZnO layer followed by surface activation promotes homogeneous surface nucleation and the growth of a low-defect ZIF-8 tubular membrane that exhibits superb gas permeation and permselectivity.
When is Diabetes?—A New Look at Diagnostic Criteria for Diabetes Mellitus (1980)

While the diagnosis of diabetes mellitus is usually straightforward because of typical symptoms and signs along with an unequivocally high plasma glucose, there is still considerable controversy about diagnostic criteria for diabetes based on the oral glucose tolerance test (OGTT). Recently there has been world-wide recognition of the need for standardisation of these criteria. A new set of diagnostic criteria, based on prospective and cross-sectional epidemiologic studies have now been recommended by a World Health Organisation Expert Committee on Diabetes. The new criteria set out recommendations for normal subjects, those with impaired glucose tolerance (a borderline group), and diabetics. The use of these criteria should result in uniform reporting of the OGTT, as well as reducing to a minimum the mis-diagnosis of diabetes.
GABAA Receptor Subtypes and the Abuse‐Related Effects of Ethanol in Rhesus Monkeys: Experiments with Selective Positive Allosteric Modulators (2019)

Our results confirm prior findings regarding the respective roles of α1GABA<sub>A</sub> and α5GABA<sub>A</sub> receptors in the discriminative stimulus effects of EtOH and, further, suggest a key facilitatory role for α3GABA<sub>A</sub> and potentially α2GABA<sub>A</sub> receptors in several abuse-related effects of EtOH in monkeys. Moreover, they reveal a potential role for these latter subtypes in EtOH's sedative effects.
Bimetallic complexes containing the bis(tetramethylcyclopentadienyl)ethane ligand (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBimetallic complexes containing the bis(tetramethylcyclopentadienyl)ethane ligandJohn F. Buzinkai and Richard R. SchrockCite this: Inorg. Chem. 1989, 28, 14, 2837–2846Publication Date (Print):July 1, 1989Publication History Published online1 May 2002Published inissue 1 July 1989https://pubs.acs.org/doi/10.1021/ic00313a029https://doi.org/10.1021/ic00313a029research-articleACS PublicationsRequest reuse permissionsArticle Views179Altmetric-Citations25LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
3.7μW 0.8V VCO-integrator-based high-efficiency capacitor-free low-dropout voltage regulator (2016)

This paper presents a VCO-integrator-based high-efficiency low-dropout voltage regulator (LDO). Due to the integrator based architecture the LDO has higher efficiency, lower input voltage and better load regulation than traditional operational-amplifier based implementations. The proposed LDO was fabricated in 180nm CMOS technology along with a traditional operational-amplifier based design for comparison. The proposed LDO consumes a power of 3.7μW at 0.8V input and can achieve a drop-out voltage of 100mV and a load regulation of 8.8μV/mA. The proposed LDO is capacitor-free.
Understanding the cochlear implant environment by mapping perilymph proteomes from different species (2016)

Cochlear implants operate within a bony channel of the cochlea, bathed in a fluid known as the perilymph. The perilymph is a complex fluid containing ions and proteins, which are known to actively interact with metallic electrodes. To improve our understanding of how cochlear implant performance varies in preclinical in vivo studies in comparison to human trials and patient outcomes, the protein composition (or perilymph proteome) is needed. Samples of perilymph were gathered from feline and guinea pig subjects and analyzed using liquid chromatography with tandem mass spectrometry (LC-MS/MS) to produce proteomes and compare against the recently published human proteome. Over 64% of the proteins in the guinea pig proteome were found to be common to the human proteome. The proportions of apolipoproteins, enzymes and immunoglobulins showed little variation between the two proteomes, with other classes showing similarity. This establishes a good basis for comparison of results. The results for the feline profile showed less similarity with the human proteome and would not provide a quality comparison. This work highlights the suitability of the guinea pig to model the biological environment of the human cochlear and the need to carefully select models of the biological environment of a cochlear implant to more adequately translate in vitro and in vivo studies to the clinic.