gebhart/patrepeval huggingface dataset:
Rogue athletes and recombinant DNA technology: challenges for doping control (2007)

The quest for athletic excellence holds no limit for some athletes, and the advances in recombinant DNA technology have handed these athletes the ultimate doping weapons: recombinant proteins and gene doping. Some detection methods are now available for several recombinant proteins that are commercially available as pharmaceuticals and being abused by dopers. However, researchers are struggling to come up with efficient detection methods in preparation for the imminent threat of gene doping, expected in the 2008 Olympics. This Forum article presents the main detection strategies for recombinant proteins and the forthcoming detection strategies for gene doping as well as the prime analytical challenges facing them.
Comparison of Elisa-Based Tyrosine Kinase Assays for Screening EGFR Inhibitors (2005)

Receptor tyrosine kinases (PTKs) play key roles in the pathogenesis of numerous human diseases, including cancer, and therefore PTK inhibitors are currently under intense investigation as potential drug candidates. PTK inhibitor screening data are, however, poorly comparable because of the different assay technologies used. Here we report a comparison of ELISA-based assays for screening epidermal growth factor receptor (EGFR) tyrosine kinase (TK) inhibitory compound libraries to study interassay variations. All assays were based on the same protocol, except for the source of EGFR-TK enzymes. In the first protocol, the enzyme was isolated from A431 cells without affinity purification. In the second protocol, commercial EGFR-TK (Sigma) isolated from A431 cells by affinity-purification was employed. In the third protocol, an enzyme preparation obtained from a recombinant (Baculovirus transfected Sf9 cells) expression system was used. All assays employed the synthetic peptide substrate poly-(Glu,Tyr)l:4 and an ELISA-based system to detect phosphorylated tyrosine residues by a monoclonal antibody. We observed significant differences in both the activity of the enzymes and in the EGFR-TK inhibitory effect of our reference compound PD153035. The differences were significant in case of A431 cell lysate compared to affinity purified EGFR-TKs derived from either A431 cells or Baculovirus transfected Sf9 cells, whereas the latter two showed comparable results. Our data suggest that differences in terms of interassay variation are not related to the source of the enzyme but to its purity; changes in the mode of detection can markedly influence the reproducibility of results. In conclusion, normalization of the EGFR activity used for inhibitor screening and standardization of detection methods enable safe comparison of data.
Tokamak pump limiter experiments and engineering (1986)

The rapid progress in the engineering, theory, and experimental results of pump limiters has led to plans for the application of these devices in new experiments over the next few years. The full toroidal belt ALT-II project, and pump limiters in D-IIID, JT-60, and Tore Supra are in the design and construction phase. Possible experiments on the Tokamak Fusion Test Reactor, the Joint European Torus, and the Advanced Toroidal Facility may be implemented soon. All of these experiments will utilize the demonstrated particle and heat-removal capabilities of pump limiters.
Visual-Inertial Scene Representations. (2016)

We describe a representation of a scene that captures geometric and semantic attributes of objects within, along with their uncertainty. Objects are assumed persistent in the scene, and their likelihood computed from intermittent visual data using a convolutional architecture, integrated within a Bayesian filtering framework with inertials and a context model. Our method yields a posterior estimate of geometry (attributed point cloud and associated uncertainty), semantics (identities and co-occurrence), and a point-estimate of topology for a variable number of objects within the scene, implemented causally and in real-time on commodity hardware.
Mobilitalea sibirica gen. nov., sp. nov., a halotolerant polysaccharide-degrading bacterium (2014)

A novel strictly anaerobic, halotolerant, organotrophic bacterium, strain P3M-3(T), was isolated from a microbial mat formed under the flow of hot water emerging from a 2775 m-deep well in Tomsk region (western Siberia, Russia). Cells of strain P3M-3(T) were straight and curved rods, 0.2-0.4 µm in width and 1.5-20 µm in length. Strain P3M-3(T) grew optimally at 37 °C, pH 7.0-7.5 and in a NaCl concentration of 15 g l(-1). Under optimum growth conditions, the doubling time was 1 h. The isolate was able to ferment a variety of mono-, di- and polysaccharides, including microcrystalline cellulose. Acetate, ethanol, H2 and CO2 were the main products of glucose fermentation. The DNA G+C content was 33.4 mol%. 16S rRNA gene-based phylogenetic analysis showed that strain P3M-3(T) was a member of family Lachnospiraceae, whose representatives are also found in Clostridium cluster XIVa. 16S rRNA gene sequence similarity with Clostridium jejuense HY-35-12(T), the closest relative, was 93.9%. A novel genus and species, Mobilitalea sibirica gen. nov., sp. nov., are proposed based on phylogenetic analysis and physiological properties of the novel isolate. The type strain of the type species is P3M-3(T) ( = DSM 26468(T) = VKM B-2804(T)).
A novel stereoscopic display technique with improved spatial and temporal properties (2014)

Common stereoscopic 3D (S3D) displays utilize either spatial or temporal interlacing to send different images to each eye. Temporal interlacing sends content to the left and right eyes alternatingly in time, and is prone to artifacts such as flicker, unsmooth motion, and depth distortion. Spatial interlacing sends even pixel rows to one eye and odd rows to the other eye, and has a lower effective spatial resolution than temporal interlacing unless the viewing distance is large. We propose a spatiotemporal hybrid protocol that interlaces the left- and right-eye views spatially, but the rows corresponding to each eye alternate every frame. We performed psychophysical experiments to compare this novel stereoscopic display protocol to existing methods in terms of spatial and temporal properties. Using a haploscope to simulate the three protocols, we determined perceptual thresholds for flicker, motion artifacts, and depth distortion, and we measured the effective spatial resolution. Spatial resolution is improved, flicker and motion artifacts are reduced, and depth distortion is eliminated. These results suggest that the hybrid protocol maintains the benefits of spatial and temporal interlacing while eliminating the artifacts, thus creating a more realistic viewing experience.
Fully Connected Reconfigurable Intelligent Surface Aided Rate-Splitting Multiple Access for Multi-User Multi-Antenna Transmission (2022)

Rate-splitting multiple access (RSMA) has been recognized as a promising and powerful multiple access (MA) scheme, non-orthogonal transmission framework and interference management strategy for 6G. Inspired by the appealing spectral efficiency gain achieved by RSMA over conventional MA schemes in multi-user multi-antenna transmission, in this paper we introduce RSMA to reconfigurable intelligent surface (RIS)-aided multiple-input single-out (MISO) broadcast channel (BC). To further enhance the spectral efficiency, a more generalized RIS architecture called fully connected RIS is considered. By jointly optimizing the scattering matrix of the fully connected RIS and the transmit beamformers to maximize the sum-rate, we show that the proposed fully connected RIS aided RSMA transmission scheme significantly improves the spectral efficiency compared with the conventional single connected RIS schemes and the schemes without RIS. It acts as a new benchmark for linearly precoded multi-user multi-antenna networks.
Novel family members of CYP109 from Sorangium cellulosum So ce56 exhibit characteristic biochemical and biophysical properties (2013)

The members of the CYP109 family (CYP109C1, CYP109C2, and CYP109D1) from Sorangium cellulosum So ce56 are among the 21 P450 enzymes, of which only CYP109D1 and CYP264B1 have so far been functionally characterized. Here, we attempted to characterize two other P450s (CYP109C1 and CYP109C2) for the first time and compare their biochemical, biophysical, and functional properties to those of the fatty acid hydroxylating CYP109D1. Considering the physiological importance of fatty acids, we investigated saturated fatty acid binding and conversion for all members of the CYP109 family. The interaction between the CYP109 members and different autologous/heterologous redox partners was compared using Biacore measurements in which only CYP109D1 and bovine adrenodoxin (Adx) formed a complex. Surprisingly, this interaction was similarly efficient as the interaction of Adx with its mammalian redox partners. The in vitro reconstitution assays showed no activity when using CYP109C1, although substrate binding was demonstrated; also, there was subterminal hydroxylation of saturated fatty acids, when using CYP109C2 and CYP109D1, where CYP109D1 was a much more efficient fatty acid hydroxylase. Interestingly, the hydroxylation position moved inside the fatty acid chain when using long-chain fatty acids, thus producing possible precursors for physiologically important products.
Cooperative performance enhancement using QUIC tunneling in 5G cellular networks (2021)

Multiplexed Application Substrate over QUIC Encryption (MASQUE) is a new protocol mechanism that is currenty under standardization in the IETF. MASQUE defines an extension to the HTTP CONNECT method in order to support QUIC-based tunneling and forwarding of UDP and IP traffic. In this paper we discuss use cases for a MASQUE-based proxy setup that addresses challenges in performance optimization in cellular networks. The presented use cases realize different services based on the supported level of cooperation between the three involved parties, i.e., the client, the proxy, and the target server.
Nonlinear Programming Methods for Distributed Optimization (2015)

In this paper we investigate how standard nonlinear programming algorithms can be used to solve constrained optimization problems in a distributed manner. The optimization setup consists of a set of agents interacting through a communication graph that have as common goal the minimization of a function expressed as a sum of (possibly non-convex) differentiable functions. Each function in the sum corresponds to an agent and each agent has associated an equality constraint. By re-casting the distributed optimization problem into an equivalent, augmented centralized problem, we show that distributed algorithms result naturally from applying standard nonlinear programming tech- niques. Due to the distributed formulation, the standard assumptions and convergence results no longer hold. We emphasize what changes are necessary for convergence to still be achieved for three algorithms: two algorithms based on Lagrangian methods, and an algorithm based the method of multipliers. The changes in the convergence results are necessary mainly due to the fact that the (local) minimizers of the lifted optimization problem are not regular, as a results of the distributed formulation. Unlike the standard algorithm based on the method of multipliers, for the distributed version we cannot show that the theoretical super-linear convergence rate can be achieved.
LDPC SD-FEC Coding in the Nonlinear Domain (2014)

We describe behavior of soft decision (SD) forward error correction (FEC) with non-Gaussian noise which is observed in nonlinear transmission with higher order modulation format and digital nonlinear compensator with inadequate parameter.
Diamidodipyrrins: Versatile Bipyrrolic Ligands with Multiple Metal Binding Modes (2008)

A straightforward, facile synthesis of diamidodipyrromethenes (diamidodipyrrins, DADP (R,R')) is presented. These tetradentate ligands readily form complexes with metal ions such as Ni (2+) and Cu (2+) and can adopt different binding modes with these metals. One version of the ligand (DADP (Ph, iPr )) has been structurally characterized in its "free base" form, as a HBr salt, and as the Ni (2+) and Cu (2+) complexes. A symmetric NNOO donor set is found for the Cu (2+) complex in the solid state, involving two carbonyl oxygen atoms and two dipyrrin nitrogen atoms, and this coordination mode has been confirmed in solution by electron paramagnetic resonance. An asymmetric NNNO binding mode found for the Ni (2+) complex in the solid state persists in solution as revealed by (1)H NMR. The HBr salt form of the ligand shows an intriguing hydrogen-bonded head-to-head dimer arrangement. Experiments show that Cu (2+), but not Ni (2+), can mediate the rapid oxidation of the diamidodipyrromethane precursors to the diamidodipyrromethene ligands in the presence of dioxygen. The work here shows that diamidodipyrrins are a versatile new class of ligands in the area of nonporphyrinic pyrrole-based compounds that merit further investigation.
Nanopore sequencing and assembly of a human genome with ultra-long reads (2017)

Abstract Nanopore sequencing is a promising technique for genome sequencing due to its portability, ability to sequence long reads from single molecules, and to simultaneously assay DNA methylation. However until recently nanopore sequencing has been mainly applied to small genomes, due to the limited output attainable. We present nanopore sequencing and assembly of the GM12878 Utah/Ceph human reference genome generated using the Oxford Nanopore MinION and R9.4 version chemistry. We generated 91.2 Gb of sequence data (∼30× theoretical coverage) from 39 flowcells. De novo assembly yielded a highly complete and contiguous assembly (NG50 ∼3Mb). We observed considerable variability in homopolymeric tract resolution between different basecallers. The data permitted sensitive detection of both large structural variants and epigenetic modifications. Further we developed a new approach exploiting the long-read capability of this system and found that adding an additional 5×-coverage of ‘ultra-long’ reads (read N50 of 99.7kb) more than doubled the assembly contiguity. Modelling the repeat structure of the human genome predicts extraordinarily contiguous assemblies may be possible using nanopore reads alone. Portable de novo sequencing of human genomes may be important for rapid point-of-care diagnosis of rare genetic diseases and cancer, and monitoring of cancer progression. The complete dataset including raw signal is available as an Amazon Web Services Open Dataset at: https://github.com/nanopore-wgs-consortium/NA12878 .
The effect of methyl-lidocaine on the biosynthesis of phospholipids de novo in the isolated hamster heart (1992)

Methyl-lidocaine is an amphiphilic agent which has been used as an experimental anti-arrhythmic drug. When hamster hearts were perfused with labelled glycerol, the presence of methyl-lidocaine in the perfusate was found to enhance the labelling in phosphatidylserine, phosphatidylinositol, diacylglycerol and triacylglycerol. However, the labelling of phosphatidylcholine and phosphatidylethanolamine was not significantly changed by methyl-lidocaine treatment. Assays in vitro for the enzymes involved in the synthesis of neutral lipids and acidic phospholipids revealed that phosphatidate phosphatase and CTP: phosphatidate cytidylyltransferase activities were stimulated by methyl-lidocaine. The intracellular pool sizes of diacylglycerol and CDP-diacylglycerol were also elevated. We postulate that the enhanced syntheses of the neutral lipids and acidic phospholipids in the methyl-lidocaine-perfused heart were mediated via the direct activation of the key enzymes in the biosynthesis of these lipids de novo.
Spectrally-modulated full-field optical coherence microscopy for ultrahigh-resolution endoscopic imaging (2006)

Full-field optical coherence microscopy (FFOCM) utilizes coherence gating to obtain high-resolution optical sections in thick tissues. FFOCM is an attractive technology for endoscopic microscopy at the cellular level since it does not require a high NA objective lens or beam scanning and is therefore particularly amenable to miniaturization. In this manuscript, we present a novel scheme for conducting FFOCM that utilizes spectrally modulated, spatially incoherent illumination and a static Linnik interferometer. This approach is advantageous for endoscopic microscopy since it allows FFOCM to be conducted through a single multimode fiber optic imaging bundle and does not require moving parts in the endoscope probe. Images acquired from biological samples in free space demonstrate that this new method provides the same detailed microscopic structure as that of conventional FFOCM. High-resolution images were also obtained through a multimode fiber bundle, further supporting the potential of this method for endoscopic microscopy.
Laser optogalvanic effect in a pure iodine discharge (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLaser optogalvanic effect in a pure iodine dischargeC. T. Rettner, C. R. Webster, and R. N. ZareCite this: J. Phys. Chem. 1981, 85, 9, 1105–1107Publication Date (Print):April 1, 1981Publication History Published online1 May 2002Published inissue 1 April 1981https://pubs.acs.org/doi/10.1021/j150609a008https://doi.org/10.1021/j150609a008research-articleACS PublicationsRequest reuse permissionsArticle Views84Altmetric-Citations23LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
In Situ Observation of Random Solid Solution Zone in LiFePO4 Electrode (2014)

Nanostructured LiFePO4 (LFP) electrodes have attracted great interest in the Li-ion battery field. Recently there have been debates on the presence and role of metastable phases during lithiation/delithiation, originating from the apparent high rate capability of LFP batteries despite poor electronic/ionic conductivities of bulk LFP and FePO4 (FP) phases. Here we report a potentiostatic in situ transmission electron microscopy (TEM) study of LFP electrode kinetics during delithiation. Using in situ high-resolution TEM, a Li-sublattice disordered solid solution zone (SSZ) is observed to form quickly and reach 10-25 nm × 20-40 nm in size, different from the sharp LFP|FP interface observed under other conditions. This 20 nm scale SSZ is quite stable and persists for hundreds of seconds at room temperature during our experiments. In contrast to the nanoscopically sharp LFP|FP interface, the wider SSZ seen here contains no dislocations, so reduced fatigue and enhanced cycle life can be expected along with enhanced rate capability. Our findings suggest that the disordered SSZ could dominate phase transformation behavior at nonequilibrium condition when high current/voltage is applied; for larger particles, the SSZ could still be important as it provides out-of-equilibrium but atomically wide avenues for Li(+)/e(-) transport.
Impact of Radiation on the Wall Heat Load at a Test Bench Gas Turbine Combustion Chamber: Measurements and CFD Simulation (2007)

Experimental and numerical work has been carried out to determine the wall heat load at the liner structure of a model gas turbine combustion chamber. Measured cross-sectional profiles of the velocity and temperature field inside the chamber could be used to validate various CFD calculations of the combustion flow. It turned out that only a special treatment of the thermal boundary conditions at all liner walls would actually lead to appropriate values of the wall heat flux. Radiation modeling included two radiative properties models (SG single gray gas and WSSG weighted sum of gray gases) and three radiation transport models (P1, DT discrete transfer, MC Monte Carlo). The performance of the WSGG model has been assessed with charts and the impact of the radiation on the liner wall temperature distribution has been studied. The experimental values are matched within 3% deviation with the best combination of transport and radiation property models. The radiation contributes to 20-30% of the total wall heat flux. The present approach enables Siemens PG to access the thermal design of combustors more precisely.
α-Lipoic Acid: A Multifunctional Antioxidant That Improves Insulin Sensitivity in Patients with Type 2 Diabetes (2000)

Alpha-Lipoic acid (LA) is a disulfide compound that is produced in small quantities in cells, and functions naturally as a co-enzyme in the pyruvate dehydrogenase and alpha-ketoglutarate dehydrogenase mitochondrial enzyme complexes. In pharmacological doses, LA is a multifunctional antioxidant. LA has been used in Germany for over 30 years for the treatment of diabetes-induced neuropathy. In patients with type 2 diabetes, recent studies have reported that intravenous (i.v.) infusion of LA increases insulin-mediated glucose disposal, whereas oral administration of LA has only marginal effects. If the limitations of oral therapy can be overcome, LA could emerge as a safe and effective adjunctive antidiabetic agent with insulin sensitizing activity.
Dopamine D4 receptor‐deficient mice, congenic on the C57BL/6J background, are hypersensitive to amphetamine (2004)

Mice lacking the dopamine D4 receptor subtype (D4R-/-) are supersensitive to methamphetamine and cocaine. We sought to expand and refine earlier experiments performed on F2 generation D4R-/- mice by lengthening the behavioral session, utilizing an N10 D4R-/- incipient congenic C57BL/6J line (D4R-/- mice backcrossed with wildtype C57BL/6J mice for 10 successive generations), and investigating whether dopamine D4Rs are necessary for the expression of behavioral sensitization to amphetamine. The D4R-/- mice demonstrated an enhanced and dose-dependent increase in amphetamine-stimulated activity compared to wildtype mice following acute administrations of amphetamine. For the behavioral sensitization experiments, separate groups of mice received either repeated administrations of the same dose of amphetamine or a subthreshold dose of amphetamine (2 mg/kg) 28 days following pretreatment with either saline, 1.0, 3.0, or 10.0 mg/kg amphetamine. The D4R-/- mice displayed an enhanced dose-dependent sensitized response to repeated amphetamine administrations compared to their wildtype littermates in both behavioral sensitization paradigms. Our present results further support the importance of dopamine D4Rs in psychostimulant-mediated locomotion and neural plasticity.