gebhart/patrepeval huggingface dataset:
The Relationship Among Carbon Dioxide Pneumoperitoneum, Vasopressin Release, and Hemodynamic Changes (1999)

We assessed the role of vasopressin (VP) for the hemodynamic response to pneumoperitoneum in pigs.Four groups of anesthetized pigs were investigated. Nine pigs were intraabdominally insufflated with CO2 and eight were intraabdominally insufflated with argon; eight pigs received an IV injection of 1 mg/kg SR 49059, a VP antagonist, before CO2 insufflation; and six pigs received SR 49059 alone. Hemodynamics, plasma concentrations of VP and vasoactive hormones, and PaCO2 were measured. Data were analyzed by using analysis of variance, Student's t-test, and Mann-Whitney U-test. Five minutes after insufflation, changes in systemic vascular resistance (SVR) were significantly correlated with changes in VP (r = 0.72; P = 0.005) but not with changes in epinephrine, norepinephrine, renin activity, or PaCO2. SVR increased during CO2 insufflation but not during argon insufflation or CO2 insufflation with a preceding infusion of SR 49059. The SR 49059 injection itself resulted in increases in heart rate and cardiac output and decreases in blood pressure and SVR. We conclude that, during CO2 pneumoperitoneum in pigs, absorbed CO2 initiates a pathophysiological process that stimulates VP release. Hence, VP most likely plays a key role in the hemodynamic response to a CO2-induced pneumoperitoneum. Implications: Intraabdominal insufflation of CO2 is associated with hemodynamic and hormonal changes. Investigating CO2 and argon-insufflated pigs and using a vasopressin antagonist, we found that CO2 insufflation released vasopressin, which, in turn, induced hemodynamic perturbances. (Anesth Analg 1999;89:278-83)
TYK2-STAT1 Pathway Positively Regulates BCL2 Gene Expression in T-Cell Acute Lymphoblastic Leukemia (2012)

Abstract Abstract 1470 To discover oncogenic pathways that are characteristically deregulated in T-cell acute lymphoblastic leukemia (T-ALL), we performed RNA interference screens both in T-ALL cell lines and primary specimens. We found that the JAK tyrosine kinase family member, TYK2, and its downstream effector, STAT1, are each required for the survival of T-ALL cells. To identify the effector molecules downstream of the TYK2-STAT1 pathway in T-ALL, we analyzed global gene expression profiles in TYK2-dependent T-ALL cell lines after silencing of TYK2 or STAT1. As expected, gene set enrichment analysis revealed that genes downregulated by TYK2 knockdown were generally also downregulated by knockdown of STAT1. Importantly, we found that expression of the anti-apoptotic gene BCL2 was significantly downregulated after silencing of both TYK2 and STAT1. Analysis by quantitative PCR of additional T-ALL cell lines revealed that silencing of TYK2 resulted in significant reductions of BCL2 mRNA expression in multiple TYK2-dependent cell lines. Expression of the wild-type but not the kinase-dead TYK2 protein was sufficient to rescue BCL2 protein expression and to prevent apoptosis after knockdown of endogenous TYK2, indicating that the tyrosine kinase activity of TYK2 is required for BCL2 upregulation. Similarly, expression of the shRNA-resistant wild-type STAT1A protein partially rescued BCL2 protein expression and prevented apoptosis, while a variant of STAT1A (Y701F) that is incapable of becoming phosphorylated on a requisite tyrosine residue did not rescue BCL2 levels. Taken together, our findings indicate that aberrant activation of a TYK2-STAT1 pathway upregulates BCL2 expression in T-ALL cells, and that the T-ALL cells develop pathway dependence, in that they require these sustained high levels BCL2 expression for survival. Disclosures: No relevant conflicts of interest to declare.
Security And The National Telecommunications Infrastructure (2005)

The US telecommunications infrastructure is a very complex system of interconnected heterogeneous networks that include both wireline and wireless systems. Because of the vital services provided by this infrastructure, there is a need to protect it against a wide range of mishaps. Such mishaps include terrorist attacks, malicious code, natural disasters, unauthorized access to information and other incidents which can seriously disrupt the networks or reduce the confidence level of the information traversing or residing in the infrastructure. This Panel will explore several security related issues that are directly related to the telecommunications infrastructure and the challenges and opportunities they offer.
Supplementary Figure Legends 1-3 from <i>Aurora A</i> Is a Repressed Effector Target of the Chromatin Remodeling Protein INI1/hSNF5 Required for Rhabdoid Tumor Cell Survival (2023)

Supplementary Figure Legends 1-3 from &lt;i&gt;Aurora A&lt;/i&gt; Is a Repressed Effector Target of the Chromatin Remodeling Protein INI1/hSNF5 Required for Rhabdoid Tumor Cell Survival
MS-275 (Entinostat) Promotes Radio-Sensitivity in PAX3-FOXO1 Rhabdomyosarcoma Cells (2021)

Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma of childhood. About 25% of RMS expresses fusion oncoproteins such as PAX3/PAX7-FOXO1 (fusion-positive, FP) while fusion-negative (FN)-RMS harbors RAS mutations. Radiotherapy (RT) plays a crucial role in local control but metastatic RMS is often radio-resistant. HDAC inhibitors (HDACi) radio-sensitize different cancer cells types. Thus, we evaluated MS-275 (Entinostat), a Class I and IV HDACi, in combination with RT on RMS cells in vitro and in vivo. MS-275 reversibly hampered cell survival in vitro in FN-RMS RD (RASmut) and irreversibly in FP-RMS RH30 cell lines down-regulating cyclin A, B, and D1, up-regulating p21 and p27 and reducing ERKs activity, and c-Myc expression in RD and PI3K/Akt/mTOR activity and N-Myc expression in RH30 cells. Further, MS-275 and RT combination reduced colony formation ability of RH30 cells. In both cell lines, co-treatment increased DNA damage repair inhibition and reactive oxygen species formation, down-regulated <i>NRF2</i>, <i>SOD</i>, <i>CAT</i> and <i>GPx4</i> anti-oxidant genes and improved RT ability to induce G2 growth arrest. MS-275 inhibited in vivo growth of RH30 cells and completely prevented the growth of RT-unresponsive RH30 xenografts when combined with radiation. Thus, MS-275 could be considered as a radio-sensitizing agent for the treatment of intrinsically radio-resistant PAX3-FOXO1 RMS.
Can Single-Walled Carbon Nanotube Diameter Be Defined by Catalyst Particle Diameter? (2019)

The need of designing and controlling single-walled carbon nanotube (SWCNT) properties is a challenge in a growing nanomaterials-related industry. Recently, great progress has been made experimentally to selectively control SWCNT diameter and chirality. However, there is not yet a complete understanding of the synthesis process, and there is a lack of mathematical models that explain nucleation and diameter selectivity of stable carbon allotropes. Here, in situ analysis of chemical vapor deposition SWCNT synthesis confirms that the nanoparticle-to-nanotube diameter ratio varies with the catalyst particle size. It is found that the tube diameter is larger than that of the particle below a specific size (dc ≈ 2 nm) and above this value is smaller than particle diameters. To explain these observations, we develop a statistical mechanics based model that correlates possible energy states of a nascent tube with the catalyst particle size. This model incorporates the equilibrium distance between the nucleating SWCNT layer and the metal catalyst (e.g., Fe, Co, and Ni) evaluated with density functional theory (DFT) calculations. The theoretical analysis explains and predicts the observed correlation between tube and solid particle diameters during growth of supported SWCNTs. This work also brings together previous observations related to the stability condition for SWCNT nucleation. Tests of the model against various published data sets and our own experimental results show good agreement, making it a promising tool for evaluating SWCNT synthesis processes.
Patterning Flexible Substrates Using Surface Relief Structures in Azobenzene Functionalized Polymer Films (2008)

A novel method for maskless micro-patterning of polymeric substrates is presented. First, an azobenzene functionalized polymer film is spin-coated on a Poly (ethylene terephthalate) (PET) sheet. Then surface relief structures are optically inscribed on the polymer film by interference of laser beams. The patterned azobenzene functionalized film is then etched in the plasma chamber such that the gratings are transferred to the PET substrate. Finally, any remaining azobenzene functionalized polymer is dissolved away using an appropriate solvent. This method of patterning can be broadly applied to a variety of flexible/polymeric substrates and the resolution is not limited by the substrate thermo-mechanical properties.
Spatial distribution of interictal spikes fluctuates over time and localizes seizure onset (2019)

The location of interictal spikes is used to aid surgical planning in patients with medically refractory epilepsy; however, their spatial and temporal dynamics are poorly understood. In this study, we analysed the spatial distribution of interictal spikes over time in 20 adult and paediatric patients (12 females, mean age = 34.5 years, range = 5-58) who underwent intracranial EEG evaluation for epilepsy surgery. Interictal spikes were detected in the 24 h surrounding each seizure and spikes were clustered based on spatial location. The temporal dynamics of spike spatial distribution were calculated for each patient and the effects of sleep and seizures on these dynamics were evaluated. Finally, spike location was assessed in relation to seizure onset location. We found that spike spatial distribution fluctuated significantly over time in 14/20 patients (with a significant aggregate effect across patients, Fisher's method: P < 0.001). A median of 12 sequential hours were required to capture 80% of the variability in spike spatial distribution. Sleep and postictal state affected the spike spatial distribution in 8/20 and 4/20 patients, respectively, with a significant aggregate effect (Fisher's method: P < 0.001 for each). There was no evidence of pre-ictal change in the spike spatial distribution for any patient or in aggregate (Fisher's method: P = 0.99). The electrode with the highest spike frequency and the electrode with the largest area of downstream spike propagation both localized the seizure onset zone better than predicted by chance (Wilcoxon signed-rank test: P = 0.005 and P = 0.002, respectively). In conclusion, spikes localize seizure onset. However, temporal fluctuations in spike spatial distribution, particularly in relation to sleep and post-ictal state, can confound localization. An adequate duration of intracranial recording-ideally at least 12 sequential hours-capturing both sleep and wakefulness should be obtained to sufficiently sample the interictal network.
Training and adapting MLP features for Arabic speech recognition (2009)

Features derived from multilayer perceptrons (MLPs) are becoming increasingly popular for speech recognition. This paper describes various schemes for applying these features to state-of-the-art Arabic speech recognition: the use of MLP-features for short-vowel modelling in graphemic systems; rapid discriminative model training by standard PLP feature lattice reuse; and MLP feature adaptation using linear input networks (LIN). The use of rapid training using MLP features and their use for short-vowel modelling and LIN adaptation gave reductions in word error rate. However significant improvements over explicit short-vowel modelling with standard multi-pass adaptation were not obtained, although they were useful in combination.
Template recognition sequence for I protein of bacteriophage T7* (DNA initiation/primase/lagging-strand synthesis/Okazaki fragments/ (2016)

The gene 4 protein of bacteriophage T7 recog- nizes specific sequences on single-stranded DNA and then cata- lyzes the synthesis of tetraribonucleotide primers complementary to the template. With 4X174 DNA as a template, the gene 4 pro- tein enables T7 DNA polymerase (deoxynucleosidetriphos- phate:DNA deoxynucleotidyltransferase, EC 2.7.7.7) to initiate DNA synthesis at 13 major sites. DNA sequence analysis of the 5' termini of the newly synthesized DNA shows the predominant rec- ognition sequences for the gene 4 protein to be 3'-C-T-G-G-G-5' or 3'-C-T-G-G-T-5'; the products of synthesis at these sites are RNA primers having the sequences pppA-C-C-C or pppA-C-C-A. The gene 4 protein can also synthesize primers at the sequences 3'-C-T-G-G-A-5' and 3'-C-T-G-T-N-5', although these sites are used less than 10% as frequently as the predominant sites. Com- parison of the utilization of primer sites suggests that the gene 4 protein binds randomly to single-stranded DNA and then translo- cates along the DNA in a unidirectional 5'-to-3' direction with re- gard to the DNA strand in search of recognition sequences. Models are presented for the role of the gene 4 protein in the initiation of lagging-strand synthesis and in the initiation of DNA replication at the origin.
3D Measurement Method for the Tire Tip in the Laser Vision Alignment System for Automobile Wheels (2002)

The principles of the laser vision alignment is introduced,and elaborates on the method of 3D coordinates measurement of the tip,namely,multi curve fitting in the light plane with least square theory.The experimental results showed reliability and stability,with a standard error σ=0.03 mm.
Digital PWM for Multi-Level Flying Capacitor Converters with Improved Output Resolution and Flying Capacitor Voltage Controller Stability (2018)

This paper presents a flying capacitor voltage stability analysis comparing Trailing Edge (sawtooth counter) and Dual Edge (triangular counter) Digital Pulse-Width Modulation (DPWM) schemes when used on Multi-Level Flying Capacitor (ML-FC) converters, and shows that dual edge DPWM provides better stability for the flying capacitor voltage. Moreover, it presents a new, sawtooth counter based, Emulated Dual Edge DPWM scheme, that provides the same stability advantage and at the same time also has better digital pulse-width resolution than a typical triangular counter based Dual Edge DPWM. The introduced Emulated Dual Edge DPWM scheme allows the use of a simple linear compensator control scheme for the flying capacitor voltage of ML-FC converters, for the full range of operation, solving feedback instability issues that would arise with the use of trailing edge DPWM under light load operation. The introduced Emulated Dual Edge DPWM scheme was verified on a 20 W, 500 kHz, 12V-to-5V, Three-Level Flying Capacitor (3L-FC) buck converter, showing the stability of the flying capacitor control loop under light load operation and improved resolution, demonstrating its superiority over both the Trailing Edge and Dual Edge DPWM.
Integrating loss aversion into a technology acceptance model to assess the relationship between website quality and website user's behavioural intentions (2012)

Abstract Incorporating the loss aversion concept into the technology acceptance model (TAM), this paper endeavours to investigate the relationship between website quality and website user's behavioural intentions in the travel agency sector. A statistical analysis of the collected questionnaires was computed based on the 1279 usable responses from the selected websites of travel agencies. Structural equation modelling is the essential analysis methodology used to examine the hypothesised relationships among the variables. Joining the loss aversion concept, the results indicate that a decrease in website quality from the website user's expectation will decrease the perceived use of ease and usefulness towards the website and then influence website user's behavioural intentions, but that an increase in website quality has no significant effects on these two TAM constructs. The results also suggest that perceived ease of use, perceived usefulness, and attitude are acting as important mediators within the model. This study demonstrates the effect of asymmetric response of website quality on website user's behavioural intentions. A discussion of the findings including managerial implications is also presented in this paper. Keywords: website qualityloss aversiontechnology acceptance modelbehavioural intentions
Supplementary Figure 1B from Tumor Subtype-Specific Cancer–Testis Antigens as Potential Biomarkers and Immunotherapeutic Targets for Cancers (2023)

&lt;p&gt;PDF file - 516K,Gene expression heatmaps for BRCA, KIRC, GBM, LUAD and UCEC. Survival curves for KIRC, LUAD and UCEC.&lt;/p&gt;
Analysis of the adult thymus in reconstitution of T lymphocytes in HIV-1 infection (1999)

A key question in understanding the status of the immune system in HIV-1 infection is whether the adult thymus contributes to reconstitution of peripheral T lymphocytes. We analyzed the thymus in adult patients who died of HIV-1 infection. In addition, we studied the clinical course of HIV-1 infection in three patients thymectomized for myasthenia gravis and determined the effect of antiretroviral therapy on CD4(+) T cells. We found that five of seven patients had thymus tissue at autopsy and that all thymuses identified had inflammatory infiltrates surrounding lymphodepleted thymic epithelium. Two of seven patients also had areas of thymopoiesis; one of these patients had peripheral blood CD4(+) T-cell levels of <50/mm3 for 51 months prior to death. Of three thymectomized patients, one rapidly progressed to AIDS, one progressed to AIDS over seven years (normal progressor), whereas the third remains asymptomatic at least seven years after seroconversion. Both latter patients had rises in peripheral blood CD4(+) T cells after antiretroviral therapy. Most patients who died of complications of HIV-1 infection did not have functional thymus tissue, and when present, thymopoiesis did not prevent prolonged lymphopenia. Thymectomy before HIV-1 infection did not preclude either peripheral CD4(+) T-cell rises or clinical responses after antiretroviral therapy.
The Human c-Fes Tyrosine Kinase Binds Tubulin and Microtubules through Separate Domains and Promotes Microtubule Assembly (2004)

The c-Fes protein-tyrosine kinase (Fes) has been implicated in the differentiation of vascular endothelial, myeloid hematopoietic, and neuronal cells, promoting substantial morphological changes in these cell types. The mechanism by which Fes promotes morphological aspects of cellular differentiation is unknown. Using COS-7 cells as a model system, we observed that Fes strongly colocalizes with microtubules in vivo when activated via coiled-coil mutation or by coexpression with an active Src family kinase. In contrast, wild-type Fes showed a diffuse cytoplasmic localization in this system, which correlated with undetectable kinase activity. Coimmunoprecipitation and immunofluorescence microscopy showed that the N-terminal Fes/CIP4 homology (FCH) domain is involved in Fes interaction with soluble unpolymerized tubulin. However, the FCH domain was not required for colocalization with polymerized microtubules in vivo. In contrast, a functional SH2 domain was essential for microtubule localization of Fes, consistent with the strong tyrosine phosphorylation of purified tubulin by Fes in vitro. Using a microtubule nucleation assay, we observed that purified c-Fes also catalyzed extensive tubulin polymerization in vitro. Taken together, these results identify c-Fes as a regulator of the tubulin cytoskeleton that may contribute to Fes-induced morphological changes in myeloid hematopoietic and neuronal cells.
Surface Modification of Spider Silk Particles to Direct Biomolecular Corona Formation (2020)

In recent years, spider silk-based materials have attracted attention because of their biocompatibility, processability, and biodegradability. For their potential use in biomaterial applications, i.e., as drug delivery systems and implant coatings for tissue regeneration, it is vital to understand the interactions between the silk biomaterial surface and the biological environment. Like most polymeric carrier systems, spider silk material surfaces can adsorb proteins when in contact with blood, resulting in the formation of a biomolecular corona. Here, we assessed the effect of surface net charge of materials made of recombinant spider silk on the biomolecular corona composition. In-depth proteomic analysis of the biomolecular corona revealed that positively charged spider silk materials surfaces interacted predominantly with fibrinogen-based proteins. This fibrinogen enrichment correlated with blood clotting observed for both positively charged spider silk films and particles. In contrast, negative surface charges prevented blood clotting. Genetic engineering allows the fine-tuning of surface properties of the spider silk particles providing a whole set of recombinant spider silk proteins with different charges or peptide tags to be used for, for example, drug delivery or cell docking, and several of these were analyzed concerning the composition of their biomolecular corona. Taken together this study demonstrates how the surface net charge of recombinant spider silk surfaces affects the composition of the biomolecular corona, which in turn affects macroscopic effects such as fibrin formation and blood clotting.
Direct numerical and experimental determination of the group index in photonic crystal waveguides (2005)

We report on direct numerical calculations and experimental measurements of the group-index dispersion in a photonic crystal waveguide fabricated in silicon-on-insulator material. The photonic crystal is defined by a triangular arrangement of holes and the waveguide is carved out by introducing a one-row line defect. Both the numerical and experimental methods are based on the time of flight approach for an optical pulse. An increase of the group index by approximately 45 times (from 4 to 155) has been observed when approaching the cutoff of the fundamental photonic bandgap mode. Numerical 2D and 3D simulations of pulse dynamics in the waveguide made by the time-domain method shows excellent agreement with measured data in most of the band. These group index values in a photonic crystal waveguide are to the best of our knowledge the largest numbers reported so far by direct tracking of pulse propagation.
Increased erythrocyte adhesiveness/aggregation in the peripheral venous blood of patients with ischaemic heart disease and an eventful course (2001)

The EAAT is a valuable tool to disclose the presence of increased red blood cell aggregability in patients with ischaemic heart disease. Increased EAA might have prognostic implications in patients with ischaemic heart disease.
Spinal cord lesions in patients with clinically isolated syndrome (2012)

Presence of SC lesions facilitates diagnosing MS and is predictive for conversion to CDMS, especially in patients with nonspinal CIS who do not fulfill brain MRI criteria. We therefore recommend performing an SC scan in patients with nonspinal CIS who do not fulfill McDonald brain MRI criteria.
TVWS Field Trials with BF-OFDM (2018)

Spectrum crunch is a severe issue for below 6GHz wireless communications. Cognitive radio and Dynamic Spectrum access (DSA) based on geographic database are promising techniques to cope with this spectrum shortage. TV White Space (TVWS) usage relies on unlicensed secondary DSA under the principle on a non-harmful interference with incumbent users, allowing to use UHF channels whose availability is changing over time and space. In order to do so, a well-designed physical layer is required in order to reach accurate frequency localization while being highly flexible. We describe field-test experiments done with an implementation of the BF-OFDM (Block-Filtered OFDM) physical layer on versatile and portable hardware prototyping boards. Field-trials results confirm the potential of BF-OFDM for fragmented spectrum access and spectrum sharing usage.