gebhart/patrepeval huggingface dataset:
Lipid Composition of Bone Marrow Fat Cells in Vivo and in Vitro (1982)

Conference Abstract| February 01 1982 Lipid Composition of Bone Marrow Fat Cells in Vivo and in Vitro F. Malik; F. Malik 1Royal Postgraduate Medical School, London Search for other works by this author on: This Site PubMed Google Scholar M.Y. Gordon; M.Y. Gordon 1Royal Postgraduate Medical School, London Search for other works by this author on: This Site PubMed Google Scholar E.C. Gordon-Smith E.C. Gordon-Smith 1Royal Postgraduate Medical School, London Search for other works by this author on: This Site PubMed Google Scholar Clin Sci (Lond) (1982) 62 (2): 44P. https://doi.org/10.1042/cs062044P Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Cite Icon Cite Get Permissions Citation F. Malik, M.Y. Gordon, E.C. Gordon-Smith; Lipid Composition of Bone Marrow Fat Cells in Vivo and in Vitro. Clin Sci (Lond) 1 February 1982; 62 (2): 44P. doi: https://doi.org/10.1042/cs062044P Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search nav search search input Search input auto suggest search filter All ContentAll JournalsClinical Science Search Advanced Search This content is only available as a PDF. © 1982 The Biochemical Society and the Medical Research Society1982 Article PDF first page preview Close Modal You do not currently have access to this content.
Analysis of High-Efficiency Operating Modes for Piezoelectric Resonator-Based DC-DC Converters (2020)

Demand for power electronics to have smaller volume, lighter weight, and lower cost will eventually require new converter energy storage technologies with fundamentally higher power density and efficiency limits. One promising alternative energy storage mechanism is the piezoelectric resonator (PR), which offers very high power density and efficiency capabilities with emerging materials and significantly improved scaling properties compared to magnetics. PRs have been proposed for power conversion previously, but typically alongside additional magnetics and/or with limited performance. In this paper, we analyze PR-based converter operating modes that best utilize the PR for high efficiency, assuming the PR is the converter's only energy storage component. In addition, we provide easily-accessible tools for estimating the PR's charge transfer, energy transfer, and efficiency characteristics, which we verify experimentally in a 200-100 V, 25 W rated prototype. This prototype exhibits peak efficiency >99% as well as high efficiency (≥96%) across a wide range of operating points, illustrating the promise of PR-based converters for high voltage, low power applications.
A root cause analysis to identify the mechanistic drivers of immunogenicity against the anti-VEGF biotherapeutic brolucizumab (2023)

Immunogenicity against intravitreally administered brolucizumab has been previously described and associated with cases of severe intraocular inflammation, including retinal vasculitis/retinal vascular occlusion (RV/RO). The presence of antidrug antibodies (ADAs) in these patients led to the initial hypothesis that immune complexes could be key mediators. Although the formation of ADAs and immune complexes may be a prerequisite, other factors likely contribute to some patients having RV/RO, whereas the vast majority do not. To identify and characterize the mechanistic drivers underlying the immunogenicity of brolucizumab and the consequence of subsequent ADA-induced immune complex formation, a translational approach was performed to bridge physicochemical characterization, structural modeling, sequence analysis, immunological assays, and a quantitative systems pharmacology model that mimics physiological conditions within the eye. This approach revealed that multiple factors contributed to the increased immunogenic potential of brolucizumab, including a linear epitope shared with bacteria, non-natural surfaces due to the single-chain variable fragment format, and non-native drug species that may form over prolonged time in the eye. Consideration of intraocular drug pharmacology and disease state in a quantitative systems pharmacology model suggested that immune complexes could form at immunologically relevant concentrations modulated by dose intensity. Assays using circulating immune cells from treated patients or treatment-naïve healthy volunteers revealed the capacity of immune complexes to trigger cellular responses such as enhanced antigen presentation, platelet aggregation, endothelial cell activation, and cytokine release. Together, these studies informed a mechanistic understanding of the clinically observed immunogenicity of brolucizumab and associated cases of RV/RO.
CHD2variants are a risk factor for photosensitivity in epilepsy (2015)

Photosensitivity is a heritable abnormal cortical response to flickering light, manifesting as particular electroencephalographic changes, with or without seizures. Photosensitivity is prominent in a very rare epileptic encephalopathy due to de novo CHD2 mutations, but is also seen in epileptic encephalopathies due to other gene mutations. We determined whether CHD2 variation underlies photosensitivity in common epilepsies, specific photosensitive epilepsies and individuals with photosensitivity without seizures. We studied 580 individuals with epilepsy and either photosensitive seizures or abnormal photoparoxysmal response on electroencephalography, or both, and 55 individuals with photoparoxysmal response but no seizures. We compared CHD2 sequence data to publicly available data from 34 427 individuals, not enriched for epilepsy. We investigated the role of unique variants seen only once in the entire data set. We sought CHD2 variants in 238 exomes from familial genetic generalized epilepsies, and in other public exome data sets. We identified 11 unique variants in the 580 individuals with photosensitive epilepsies and 128 unique variants in the 34 427 controls: unique CHD2 variation is over-represented in cases overall (P = 2.17 × 10(-5)). Among epilepsy syndromes, there was over-representation of unique CHD2 variants (3/36 cases) in the archetypal photosensitive epilepsy syndrome, eyelid myoclonia with absences (P = 3.50 × 10(-4)). CHD2 variation was not over-represented in photoparoxysmal response without seizures. Zebrafish larvae with chd2 knockdown were tested for photosensitivity. Chd2 knockdown markedly enhanced mild innate zebrafish larval photosensitivity. CHD2 mutation is the first identified cause of the archetypal generalized photosensitive epilepsy syndrome, eyelid myoclonia with absences. Unique CHD2 variants are also associated with photosensitivity in common epilepsies. CHD2 does not encode an ion channel, opening new avenues for research into human cortical excitability.
Deoxyribonuclease-Positive Staphylococcus epidermidis Strains (1970)

Use of the agar plate test for the enzyme deoxyribonucleate 3'-nucleotidohydrolase (deoxyribonuclease) can result in frequent misdiagnosis of Staphylococcus epidermidis as S. aureus.
Establishment and characterization of human pancreatic adenocarcinoma cell line SW-1990 in tissue culture and the nude mouse. (1983)

A new tumor line derived from a human pancreatic ductal adenocarcinoma of a 56-year-old Caucasian male was established in tissue culture and the nude mouse. In tissue culture, the neoplastic cells grew as large, epithelial-like, mucin-producing cells. Injection s.c. of 1 X 10(6) cultured neoplastic cells into nude mice resulted in tumor formation histologically closely resembling the original neoplasm. Ultrastructurally, the neoplastic cells showed characteristics of ductal epithelium. The allozyme phenotypic profile of the line was established in 14 genetically determined loci, and chromosome studies showed a near-tetraploid pattern. Production of macromolecules such as lactate dehydrogenase and carcinoembryonic antigen were present in measurable amounts in culture media, tumor cell extracts, nude mouse-grown tumors, and the serum of tumor-bearing mice in amounts relative to tumor size. Pancreatic enzymes were not detected. These characteristics make tumor line SW-1990 a valuable tool in studying various aspects of the biology of human pancreatic cancer.
Real‐time electrical monitoring of reagent delivery during a subsurface amendment experiment (2013)

ABSTRACT An electrical resistivity monitoring survey was conducted on a mine heap to track reagent movement during high‐pressure injections. The injections were designed to increase the dissolution of metallic gold from low‐grade ore and enhance recovery after surface leaching had ceased. The main objective of the geoelectrical monitoring was to observe the effectiveness of the injection technique and provide feedback to optimize injection parameters in real time. Real‐time assessment was achieved by monitoring the raw output current and transfer resistance on a network of borehole electrodes installed around the injection well. It was demonstrated that the output current increased significantly on particular borehole electrodes after commencement of reagent injection, when the wetting front arrived at the electrodes. When injection ceased, the electrical current returned to the initial baseline current values. The timing and distribution of the electrodes demonstrating this behaviour varied with injection depth. The internal structure of the heap was likely a controlling factor in reagent movement. Resistance, converted to apparent resistivity, was also shown to change significantly in the region near the injection. Verification of the real‐time assessment was conducted with post‐injection time‐lapse 3D tomographic inversion. While inverse modelling provides a truer 3D representation of reagent injection, the cost was shown to be a time‐lag of 3.5 days to complete the modelling. The simplicity of monitoring the raw current output and voltage can make this a powerful tool for real‐time tracking of fluid movement in the subsurface.
ChemInform Abstract: Nonoxidative Coupling Methodology for the Synthesis of the Antitumor Bisindole Alkaloid Vinblastine and a Lower‐Half Analogue: Solvent Effect on the Stereochemistry of the Crucial C‐15/C‐18′ Bond. (1993)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Structural Analysis: Shape Information via Points-To Computation (2012)

This paper introduces a new hybrid memory analysis, Structural Analysis, which combines an expressive shape analysis style abstract domain with efficient and simple points-to style transfer functions. Using data from empirical studies on the runtime heap structures and the programmatic idioms used in modern object-oriented languages we construct a heap analysis with the following characteristics: (1) it can express a rich set of structural, shape, and sharing properties which are not provided by a classic points-to analysis and that are useful for optimization and error detection applications (2) it uses efficient, weakly-updating, set-based transfer functions which enable the analysis to be more robust and scalable than a shape analysis and (3) it can be used as the basis for a scalable interprocedural analysis that produces precise results in practice. The analysis has been implemented for .Net bytecode and using this implementation we evaluate both the runtime cost and the precision of the results on a number of well known benchmarks and real world programs. Our experimental evaluations show that the domain defined in this paper is capable of precisely expressing the majority of the connectivity, shape, and sharing properties that occur in practice and, despite the use of weak updates, the static analysis is able to precisely approximate the ideal results. The analysis is capable of analyzing large real-world programs (over 30K bytecodes) in less than 65 seconds and using less than 130MB of memory. In summary this work presents a new type of memory analysis that advances the state of the art with respect to expressive power, precision, and scalability and represents a new area of study on the relationships between and combination of concepts from shape and points-to analyses.
FLC materials optimized for high resolution magnified view and projection applications (2000)

Abstract CMOS-based microdisplays which use time-sequential color monopixels have a much higher fill factor and markedly better image quality than displays using triad subpixels. These microdisplays excel in applications requiring either compact viewers or projectors. This paper describes optical architectures used for incorporating microdisplays into near-to-eye and projector displays, and reports some figures of merit for the displays. It also describes the liquid crystal mixtures used in the displays, and details some LC dopants that can give these mixtures wider temperature ranges, faster switching speeds, and lower birefringence.
Supplementary Tables S1 - S12 from Whole Genome Sequencing Defines the Genetic Heterogeneity of Familial Pancreatic Cancer (2023)

&lt;p&gt;Supplementary Tables S1 - S12. Supplementary Table S1. Prioritization of candidate FPC susceptibility genes by private heterozygous PTVs. Supplementary Table S2. FPC patients harboring a private PTVs ASXL1, DNMT3A, and TET2. Supplementary Table S3. 87 genes analyzed in-depth in FPC patients and kindreds. Supplementary Table S4. Deleterious variants in FPC patients and kindreds. Supplementary Table S5. FPC samples with more than one deleterious variant. Supplementary Table S6. Deleterious variants in BCCS samples. Supplementary Table S7. Deleterious variants in FPC kindreds and BCCS samples. Supplementary Table S8. Differences in deleterious variants between FPC kindreds with three or more affected members and BCCS samples. Supplementary Table S9. Private heterozygous PTVs present in all sequenced affected members of a kindred. Supplementary Table S10. Deleterious variants shared between affected members of an FPC kindred. Supplementary Table S11. Somatic mutations in FPC patient tumors. Supplementary Table S12. Summary of somatic mutation data for FPC patient tumors by gene.&lt;/p&gt;
The Primary Cilium of MDCK Cells is a Flow Sensor (2001)

Abstract The primary cilium, a solitary non-motile structure projecting from the centriole of mammalian cells, has been a subject of interest of anatomists since 1898. Surprisingly, the function of the primary cilium in mammalian cells is completely unknown. Virtually all of the epithelial cells of the mammalian kidney, with the exception of the intercalated cells of the collecting duct, express a single primary cilium on their apical (lumenal) surface. These structures were ignored by physiologists until recently when the mechanical properties of the primary cilia of cultured cells of renal origin were measured and it was proposed that they could serve as flow sensors. The proposal stemmed from the observation that flow rates comparable to those observed in renal tubules resulted in a deflection of the cilium of a few microns and that the stiffness of the cilium was commensurate with mechanosensing. The primary cilia of renal tubular cells are generally about 2 - 3 μm long whereas those of cultured renal cells become much longer as the cells age and may extend 50 μm or more into the medium. MDCK cells, a cultured cell line derived from the collecting duct of the canine kidney, exhibit two cell types analogous to the principal and intercalated cells of the collecting duct. MDCK principal cells express a primary cilium that is visible by high-resolution DIC microscopy. About one week after splitting, the primary cilium of MDCK principal cells is about 8 μm long and is well suited for testing the hypothesis that the cilium serves as a mechanosensor of fluid flow.
Synthesis and burst strength of water‐swollen immunoisolatory tubules (2002)

Abstract A series of amphiphilic hydrogel tubules have been prepared by copolymerizing/crosslinking hydrophilic poly(dimethylacrylamide) segments with hydrophobic di‐, tri‐, and octamethacrylate‐telechelic polyisobutylene crosslinkers, and their elastic modulus and burst strength in the water‐swollen state were investigated. Because the burst characteristics of hydrogels have not yet been quantitatively investigated, equipment was designed and built to generate fundamental insight into the burst properties of thin‐walled (200–250 μm) narrow lumen (2–3 mm i.d.) water‐swollen tubules. The theory developed to describe quantitatively the inflation behavior of thin‐walled rubber tubules was adapted to treat our experimental observations. Changes in the burst strength, elastic modulus, and expansion during the inflation of hydrogel tubules were interpreted in terms of the molecular weight of the hydrophilic segments between crosslinking sites ( M c,hydrophilic ), which in turn was calculated according to the rubber elasticity theory. According to these investigations, the burst strength of our water‐swollen amphiphilic tubules is in the 0.2–0.5 MPa range, which is sufficient for implantation and immunoisolatory applications. © 2002 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 40: 2075–2084, 2002
Favorable Propagation with User Cluster Sharing (2020)

We examine the favorable propagation (FP) behavior of a massive multi-user multiple-input-multiple-output (MU-MIMO) system equipped with a uniform linear array (ULA), horizontal uniform rectangular array (HURA) or uniform circular array (UCA) using a ray-based channel model with user cluster sharing. We demonstrate FP for these systems and provide analytical expressions for the mean-squared distance (MSD) of the FP metric from its large-system limit for each of the aforementioned topologies. We use these results to examine the detrimental effects of user cluster sharing on FP behavior, and demonstrate the superior performance of the ULA as compared to the UCA and the HURA with equal inter-element spacing. Although cluster sharing has a negative impact on FP for finite arrays, we additionally examine the asymptotic rate of convergence to FP as a function of array size and show that this rate is unchanged with or without user cluster sharing.
Investigations on Bond Strength Development of Plasma Activated Direct Wafer Bonding with Annealing (2013)

In order to clarify the mechanism behind the bond strength enhancement at low temperatures by plasma activation the bond strength development with annealing temperature was systematically investigated. The study consisted of bonding experiments on the material system Si-SiO2 under varying process conditions. Combining experimental findings with established models we developed a better understanding of the bonding process which helps explaining the experimentally found behavior. Based on this improved understanding we can predict that plasma activated (PA) wafer bonding can be successfully employed for a larger group of materials than it is currently used.
HDAC inhibitor conjugated polymeric prodrug micelles for doxorubicin delivery (2017)

Amphiphilic diblock copolymers bearing histone deacetylase inhibitors (HDACi) (4-phenyl butyric acid and valproic acid) were synthesized by the ring-opening polymerization of γ-4-phenylbutyrate-ε-caprolactone (PBACL), γ-valproate-ε-caprolactone (VPACL), and ε-caprolactone (CL) from a poly(ethylene glycol) macroinitiator (PEG). These amphiphilic diblock copolymers self-assembled into stable pro-drug micelles and demonstrated excellent biocompatibility. High loading of doxorubicin (DOX) up to 5.1 wt% was achieved. Optimized micelles enabled sustained drug release in a concentration-dependent manner over time to expand the therapeutic window of cytotoxic small molecule drugs.
Fault Isolation of Resistive/Open 3-D Wafer Bonding Interconnects by Thermal Laser Stimulation and Light-Induced Capacitance Alteration (2020)

Abstract This paper presents novel optical beam-based defect localization approaches for resistive and open failed wafer-towafer (W2W) bonding interconnects for 3-D integration. The use of an etch back process in combination with thermal laser stimulation (TLS) and light-induced capacitance alteration (LICA) using visible laser excitation enables us to accurately pinpoint defects in high-density W2W interconnect structures down to a pitch of 2.2 µm. We confirm our results by focusedion beam (FIB) cross sectioning.
Suppressing Unspecific Cell Uptake for Targeted Delivery Using Hydroxyethyl Starch Nanocapsules (2012)

Synthesizing nanocarriers with stealth properties and delivering a "payload" to the particular organ remains a big challenge but is the prime prerequisite for any in vivo application. As a nontoxic alternative to the modification by poly(ethylene glycol) PEG, we describe the synthesis of cross-linked hydroxyethyl starch (HES, M(w) 200,000 g/mol) nanocapsules with a size range of 170-300 nm, which do not show nonspecific uptake into cells. The specific uptake was shown by coupling a folic acid conjugate as a model targeting agent onto the surface of the nanocapsules, because folic acid has a high affinity to a variety of human carcinoma cell lines which overexpress the folate receptor on the cell surface. The covalent binding of the folic acid conjugate onto HES capsules was confirmed by FTIR and NMR spectroscopy. The coupling efficiency was determined using fluorescence spectroscopy. The specific cellular uptake of the HES nanocapsules after folic acid coupling into the folate-receptor presenting cells was studied by confocal laser scanning microscopy (CLSM) and flow cytometry.
Biocompatible depolymerization of lignin to enable microbial conversion. (2021)

Abstract not provided.
alpha 2-macroglobulin adsorbed to colloidal gold: a new probe in the study of receptor-mediated endocytosis. (1981)

alpha 2-Macroglobulin (alpha 2 M) was adsorbed to colloidal gold and used as a new tool in the study of receptor-mediated endocytosis. alpha 2 M-gold is easy to prepare and is clearly visualized at the electron microscope level. When cells were incubated with alpha 2 M-gold at 0 degrees C, gold was visualized both diffusely over the cell surface and concentrated in coated pits. After cells to which alpha 2 M-gold had been bound at 0 degrees C were warmed, the gold was rapidly internalized into uncoated vesicles, previously termed receptosomes. After 30 min of incubation or longer, gold was found in small lysosomes and, later, in large lysosomes and very small vesicles in the region of the Golgi complex. This pattern of localization is similar to that previously described, using peroxidase-labeled anti-alpha 2 M antibodies. By incubating cells with both alpha 2 M-gold and vesicular stomatitis virus (VSV), we studied the internalization of these two markers simultaneously. VSV and alpha 2 M-gold rapidly clustered in the same coated pits and were internalized in the same receptosomes. Proteins and hormones adsorbed to gold may be useful in the study of receptor-mediated endocytosis.