gebhart/patrepeval huggingface dataset:
Activation and phosphorylation of the subunits of phosphorylase kinase (1972)

Research Article| November 01 1972 Activation and phosphorylation of the subunits of phosphorylase kinase P Cohen P Cohen 1Department of Biochemistry, Medical Sciences Institute, University of Dundee, Dundee DD1 4HN, U.K. Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1972) 130 (1): 5P–6P. https://doi.org/10.1042/bj1300005Pb Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Cite Icon Cite Get Permissions Citation P Cohen; Activation and phosphorylation of the subunits of phosphorylase kinase. Biochem J 1 November 1972; 130 (1): 5P–6P. doi: https://doi.org/10.1042/bj1300005Pb Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Journal Search Advanced Search This content is only available as a PDF. © 1972 The Biochemical Society1972 Article PDF first page preview Close Modal You do not currently have access to this content.
Wave farm flicker severity: Comparative analysis and solutions (2016)

This paper proposes a flicker severity study for Wave Energy Converter farms. The flicker severity is introduced and the reason why it is an important constraint for a wave farm is explained. A new representation called intrinsic flicker severity is introduced which describes the flicker severity independently of the grid. The influence of device type, its control and the sea-state on average production, flicker severity and on the ratio between flicker and production are studied with three types of devices: an Oscillating Water Column and two Direct Wave Energy Converters (two point absorbers: a Heaving Buoy and the SEAREV). The influence of the size and the placement of each unit in the wave farm is presented with a farm-unit flicker ratio, compared with the square-root of unit hypothesis (noise behavior), as a function of wave direction by taking into account wave direction dispersion. Finally, solutions are presented to reduce the flicker produced to comply with grid code requirements in order to allow grid integration of wave farms.
Isoliquiritigenin is a potent inhibitor of NLRP3 inflammasome activation and diet-induced adipose tissue inflammation (2014)

Inflammasome activation initiates the development of many inflammatory diseases, including obesity and type 2 diabetes. Therefore, agents that target discrete activation steps could represent very important drugs. We reported previously that ILG, a chalcone from Glycyrrhiza uralensis, inhibits LPS-induced NF-κB activation. Here, we show that ILG potently inhibits the activation of NLRP3 inflammasome, and the effect is independent of its inhibitory potency on TLR4. The inhibitory effect of ILG was stronger than that of parthenolide, a known inhibitor of the NLRP3 inflammasome. GL, a triterpenoid from G. uralensis, had similar inhibitory effects on NLRP3 activity, but high concentrations of GL were required. In contrast, activation of the AIM2 inflammasome was inhibited by GL but not by ILG. Moreover, GL inhibited NLRP3- and AIM2-activated ASC oligomerization, whereas ILG inhibited NLRP3-activated ASC oligomerization. Low concentrations of ILG were highly effective in IAPP-induced IL-1β production compared with the sulfonylurea drug glyburide. In vivo analyses revealed that ILG potently attenuated HFD-induced obesity, hypercholesterolemia, and insulin resistance. Furthermore, ILG treatment improved HFD-induced macrovesicular steatosis in the liver. Finally, ILG markedly inhibited diet-induced adipose tissue inflammation and IL-1β and caspase-1 production in white adipose tissue in ex vivo culture. These results suggest that ILG is a potential drug target for treatment of NLRP3 inflammasome-associated inflammatory diseases.
YPTB3816 of Yersinia pseudotuberculosis strain IP32953 is a virulence-related metallo-oligopeptidase (2016)

The results of this study provide valuable contribution to the investigation of bacterial peptidases in general, and that of metallo-oligopeptidases in particular. This is the first study demonstrating that opdA in Yersinia pseudotuberculsosis is required for pathogenicity. The data reported are important for better understanding of the role of OpdA-like enzymes in pathogenesis in bacterial infections. Characterisation of this protein may serve as a basis for the development of novel antibacterials based on specific inhibition of this peptidase activity.
A 48-kilodalton Mycoplasma fermentans membrane protein induces cytokine secretion by human monocytes (1994)

Mycoplasma fermentans is one of several Mycoplasma species that have been reported to stimulate tumor necrosis factor (TNF) secretion from monocytes. This activity has been associated primarily with the mycoplasma membrane fraction. In this article, we have characterized a membrane protein that stimulates TNF and interleukin 1 beta secretion. The TNF-releasing activity partitioned into the Triton X-114 detergent phase, suggesting that the molecules is hydrophobic. The secretion of TNF is elevated in the presence of serum, which suggests that a serum component may play a role in the interaction between this mycoplasma protein and monocytes. Treatment of monocytes with monoclonal anti-CD14 antibody had no effect on the levels of TNF-releasing activity. By using the monocyte Western blot (immunoblot) technique, we have determined the molecular mass of the active molecule to be 48 kDa. This molecule appears to be distinct from the recently described family of variable lipoproteins of M. fermentans. Mycoplasma particulate material treated with proteinase K lost all inducing activity, whereas lipoprotein lipase-treated samples retained some level of activity.
An Adaptive Appearance Model Approach for Model-based Articulated Object Tracking (2006)

The detection and tracking of three-dimensional human body models has progressed rapidly but successful approaches typically rely on accurate foreground silhouettes obtained using background segmentation. There are many practical applications where such information is imprecise. Here we develop a new image likelihood function based on the visual appearance of the subject being tracked. We propose a robust, adaptive, appearance model based on the Wandering-Stable-Lost framework extended to the case of articulated body parts. The method models appearance using a mixture model that includes an adaptive template, frame-to-frame matching and an outlier process. We employ an annealed particle filtering algorithm for inference and take advantage of the 3D body model to predict selfocclusion and improve pose estimation accuracy. Quantitative tracking results are presented for a walking sequence with a 180 degree turn, captured with four synchronized and calibrated cameras and containing significant appearance changes and self-occlusion in each view.
GaN laser diodes for quantum sensors, optical atomic clocks and systems (2018)

Quantum technologies containing key GaN laser components will enable a new generation of precision sensors, optical atomic clocks and secure communication systems for defence applications such as next generation navigation, gravity mapping and timing since the AlGaInN material system allows for laser diodes to be fabricated over a wide range of wavelengths from the u.v. to the visible. We report our latest results on a range of AlGaInN diode-lasers targeted to meet the linewidth, wavelength and power requirements suitable for optical clocks and cold-atom interferometry systems. This includes the [5s<sup>2</sup>S<sub>1/2</sub>-5p<sup>2</sup>P<sub>1/2</sub>] cooling transition in strontium+ ion optical clocks at 422 nm, the [5s<sub>2</sub><sup>1</sup>S<sub>0</sub>-5p<sup>1</sup>P1] cooling transition in neutral strontium clocks at 461 nm and the [5s<sup>2</sup><sub>s1/2</sub> – 6p<sup>2</sup>P<sub>3/2</sub>] transition in rubidium at 420 nm. Several approaches are taken to achieve the required linewidth, wavelength and power, including an extended cavity laser diode (ECLD) system and an on-chip grating, distributed feedback (DFB) GaN laser diode.
Efficient Isomer-Pure Synthesis of a Benzo[b]thiophene Analogue of Pentacene, (2004)

Isomer-pure thieno[3,2-f:4,5-f ']bis[1]benzothiophene (2, n = 2) has been synthesized in an efficient four step approach without column chromatography.
Measurements of ion mobility in transformer oil: Evaluation in terms of ion drift (2012)

The aim of this paper is to develop a theoretical foundation of the reversed polarity method for measuring the mobility of ions in transformer oil. The results of the measurements of transient currents in oil, showing very distinct transient current peaks appearing after polarity reversal, are evaluated by computer simulations using an ion drift model. It is found from the analysis that the peaks in the measured currents occurred at instants much shorter than the so-called transit time that is the time for an ion to cross the oil gap between electrodes. A relation between the transit time and the current peak position is found that can be used to extract the ion mobility from data obtained with an experimental set up in which the transit time is shorter than the dielectric relaxation time of the liquid. On the other hand, for a setup providing the dielectric relaxation time shorter than the transit time, the current peak position strongly depends on the former and no simple correlation between the current peak position and transit times can be established.
Rational design of blocking groups for high triplet energy n-type host materials (2022)

The effect of blocking groups on the device lifetime and efficiency of blue phosphorescent organic light-emitting diodes (PhOLEDs) was investigated by synthesizing electron transport-type hosts with different blocking groups.
Optimal Mismatched Filtering to Address Clutter Spread from Intra-CPI Variation of Spectral Notches (2019)

It was recently shown that cognitive spectral notching of FM noise waveforms on transmit is an effective means with which to avoid relatively narrow in-band interference, such as occurs for radar/communication spectrum sharing. It was likewise observed, however, that when the notch is required to move during the coherent processing interval (CPI) to address dynamic interference, the resulting nonstationarity induces increased residual clutter after cancellation. Here we examine the impact of least-squares based mismatched filtering (MMF) to compensate for this residue. This manner of MMF is required not only to reduce the range sidelobe modulation that arises from dynamic transmit notching, but also to maintain the notch on receive so that the effect of in-band interference is minimized.
Rapid onset of adalimumab and long-term efficacy among week-8 responders in adults with moderate to severe active Ulcerative Colitis (2011)

Patients were allowed previous anti-TNF agent use. Co-primary endpoints were proportion of patients with (1) clinical remission at Week 8; (2) clinical remission at Week 52. Major secondary endpoints included sustained clinical remission at both Week 8 and 52, clinical response and mucosal healing at Week 8, Week 52, and both Week 8 and 52. At/after Week 8 patients were permitted corticosteroid tapering. Patients demonstrating inadequate response 1 could switch to openlabel (OL) 40 mg eow at Week 12. Dose escalation to 40 mg weekly was permitted for patients demonstrating inadequate response on OL administration. Efficacy analyses were conducted on the ITT population; using NRI for missing/ incomplete data. Safety analyses were conducted on the safety population RESULTS: 248 (of 494) ITT patients received adalimumab. Significantly more adalimumab-treated patients achieved clinical remission, clinical response, and mucosal healing at Week 8, Week 52, and both Week 8 and 52, compared with placebo. Among patients with corticosteroid use at baseline, significantly more adalimumab-treated patients discontinued corticosteroids before Week 52 and achieved clinical remission at Week 52, compared with placebo (13.3% vs. 5.7%, P 0.035). Among patients with prior anti-TNF agent use (40.3% of ITT patients), significantly more adalimumab-treated patients achieved clinical remission at Week 52, clinical response at Week 52, and sustained clinical response at both Week 8 and 52, compared with placebo. No deaths, tuberculosis or lymphomas were reported. Incidence rates of serious and infectious AEs were similar between placebo and adalimumab groups. CONCLUSION: Adalimumab was efficacious in inducing and maintaining clinical remission in patients with moderate-to-severe UC who did not adequately respond to conventional therapy with oral corticosteroids/immunosuppressants. The safety profile in UC was consistent with the known safety profile of adalimumab.
Vascular endothelial growth factor receptor 2 is a significant negative prognostic biomarker in triple-negative breast cancer: results from a controlled randomised trial of premenopausal breast cancer. (2009)

Abstract Abstract #1087 Background: Triple-negative breast cancer (TNB) is today an established phenotype of primary breast cancer recognized by estrogen (ER) negativity, progesterone (PgR) negativity and (human epidermal growth factor) HER2 negativity. The prognosis is poor and moreover patients with TNB dont benefit from established targeted drugs with antiestrogen therapies or trastuzumab. The role of other putative targets in TNB, like angiogenic markers and additional tyrosine kinase receptors is therefore a topic of interest in order to find effective targeted therapies improving the prognosis.&amp;#x2028; Patients and methods: In a cohort of premenopausal breast cancer (n=564) randomized to adjuvant tamoxifen or no adjuvant treatment a tissue microarray was prepared from available primary tumors (n=500) and immunhistochemical (IHC) staining was performed including ER, PgR, HER2, vascular endothelial growth factor A (VEGF-A), vascular endothelial growth factor receptor 2 (VEGFR2), epidermal receptor growth factor (EGFR) and platelet derived growth factor ß (PDGFR ß). HER2 status was additionally defined using fluorescence in situ hybridisation (FISH). Data regarding Nottingham histological grade (NHG) and Ki67, node status, tumour size and age was also included. All included patients were used for descriptive analysis, whereas only untreated patients were included in survival analysis.&amp;#x2028; Results: In the whole cohort 96 patients had a TNB phenotype characterized by low age, high NGH (p&amp;lt;0.001), high Ki67-index (p&amp;lt;0.001), T2 tumors (p=0.002), N-negativity (p=0.004. EGFR-positivity (p&amp;lt;0.001) and tumor-specific VEGFR2 expression (p=0.03). TNB phenotype was not associated with VEGF-A or stromal expression of PDGFR ß, and was a significant prognostic factor for 10-year BCSS (HR 2.1 CI: 1.4-3.3) by univariate analysis in the untreated arm. The prognosis was further explored among TNB patients and VEGFR2 was a significant negative prognostic factor (HR 2.5 CI; 1.1-5.5) for 10-year BCSS, whereas VEGF-A , EGFR and PDGFR ß were not.&amp;#x2028; Discussion: In a controlled randomized trial of premenopausal breastcancer, TNB was associated with worse prognosis. VEGFR2 was a significant prognostic biomarker for TNB patients which makes VEGFR2 a putative therapeutic target for this specific phenotype. Additional analysis of EGFR, VEGF-A and PDGFR ß did not add any prognostic information for TNB patients in this study. Citation Information: Cancer Res 2009;69(2 Suppl):Abstract nr 1087.
Electrochemical DNA Sensor Using Genetically Engineered Thermostable Pyrroloquinoline Quinone Glucose Dehydrogenase (2003)

Genetically engineered thermostable pyrroquinoline quinone glucose dehydrogenase (S415CGDH) was used for labeling probe DNA and amperometric DNA sensor was constructed and utilized for the detection of PCR amplified Salmonella virulence invA gene. The invA gene from Salmonella which accounts for many cases of food poisoning was targeted and the DNA bearing a specific sequence complementary to the invA gene was immobilized onto an Au electrode as a capture DNA S415CGDH labeled probe DNA was hybridized with the immobilized DNA at 60°C for 10 min and then the resulting electric current generated from S415CGDH by glucose addition was measured. The electric current was obtained when S415CGDH was used for labeling probe DNA but not when the native enzyme was used. The sensor response increased with the addition of glucose and 4.0 × 10-9 M of the S415CGDH labeled target DNA was detected in the presence of 29 mM glucose. The detection of PCR product was also investigated and it was successfully detected using asymmetric PCR product with sandwich method.
Divergent Regio‐ and Stereoselective Gold‐catalyzed Synthesis of α‐Fluorosulfones and β‐Fluorovinylsulfones from Alkynylsulfones (2017)

We developed a widely applicable, highly efficient synthesis of α-fluorosulfone and β-fluorovinylsulfone catalyzed by gold. Starting with alkynyl sulfone 1, an [Au]/HF/N-oxide system gives α-fluorosulfone 3 via a gold carbene intermediate, and, if no N-oxide is used, direct addition of HF to 1 gives vinyl sulfone 4 via a vinylfluoro gold intermediate. Both methods have good functional group tolerance and the reactions can be conducted in ambient atmosphere.
LFA-1 AND MAC-1 MEDIATE PULMONARY RECRUITMENT OF NEUTROPHILS AND TISSUE DAMAGE IN ABDOMINAL SEPSIS (2008)

Neutrophil-mediated lung damage is an insidious feature in septic patients, although the adhesive mechanisms behind pulmonary recruitment of neutrophils in polymicrobial sepsis remain elusive. The aim of the present study was to define the role of lymphocyte function antigen-1 (LFA-1) and membrane-activated complex 1 (Mac-1) in septic lung injury. Pulmonary edema, bronchoalveolar infiltration of neutrophils, levels of myeloperoxidase, and CXC chemokines were determined after cecal ligation and puncture (CLP). Mice were treated with monoclonal antibodies directed against LFA-1 and Mac-1 before CLP induction. Cecal ligation and puncture induced clear-cut pulmonary damage characterized by edema formation, neutrophil infiltration, and increased levels of CXC chemokines in the lung. Notably, immunoneutralization of LFA-1 or Mac-1 decreased CLP-induced neutrophil recruitment in the bronchoalveolar space by more than 64%. Moreover, functional inhibition of LFA-1 and Mac-1 abolished CLP-induced lung damage and edema. However, formation of CXC chemokines in the lung was intact in mice pretreated with the anti-LFA-1 and anti-Mac-1 antibodies. Our data demonstrate that both LFA-1 and Mac-1 regulate pulmonary infiltration of neutrophils and lung edema associated with abdominal sepsis. Thus, these novel findings suggest that LFA-1 or Mac-1 may serve as targets to protect against lung injury in polymicrobial sepsis.
Representation of cochlea within primary auditory cortex in the cat (1975)

The representation of sound frequency (and of the cochlear partition) within primary auditory cortex has been investigated with use of microelectrode-mapping techniques in a series of 25 anesthetized cats. Among the results were the following: 1) Within vertical penetrations into AI, best frequency and remarkably constant for successively studied neurons across the active middle and deep cortical layers. 2) There is an orderly representation of frequency (and of represented cochlear place) within AI. Frequency is rerepresented across the mediolateral dimension of the field. On an axis perpendicular to this plane of rerepresentation, best-frequency (represented cochlear place) changes as a simple function of cortical location. 3) Any given frequency band (or sector of the cochlear partition) is represented across a belt of cortex of nearly constant width that runs on a nearly straight axis across AI. 4) There is a disproportionately large cortical surface representation of the highest-frequency octaves (basal cochlea) within AI. 5) The primary and secondary field locations were somewhat variable, when referenced to cortical surface landmarks. 6) Data from long penetrations passing down the rostral bank of the posterior ectosylvian sulcus were consistent with the existence of a vertical unit of organization in AI, akin to cortical columns described in primary visual and somatosensory cortex. 7) Responses to tonal stimuli were encountered in fields dorsocaudal, caudal, ventral, and rostral to AI. There is an orderly representation of the cochlea within the field rostal to AI, with a reversal in best frequencies across its border with AI. 8) Physiological definitions of AI boundaries are consistent with their cytoarchitectonic definition. Some of the implications of these findings are discussed.
SQUIDs for measuring the magnetic properties of materials (1978)

SQUID magnetometers in conjunction with superconducting magnets and shields are now being used for susceptometrs that provide unique combinations of sensitivity, speed, resolution, accuracy, stability and convenience. They are being integrated into systems with automated data acquisition and closed cycle helium refrigerators. These systems promise to become increasingly important for measurements of the magnetic properties of materials.
Investigation of the Scope of a Co-Mediated O → C Ring-Contraction (2007)

A series of dihydropyrans bearing a dicobalthexacarbonyl moiety have been found to undergo an efficient Al-promoted O-->C ring-contraction process. This rearrangement reaction operates successfully for a broad range of substrates. Moreover, the potential of this approach for generating cyclopropanes from the corresponding dihydrofurans has been demonstrated.
Efficient Reliability Analysis of Processor Datapath using Atomistic BTI Variability Models (2015)

In this paper, we propose EDA methodologies for efficient, datapath-wide reliability analysis under Bias Temperature Instability (BTI). The proposed EDA flow combines the efficiency of atomistic, pseudo-transient BTI modeling with the accuracy of commercial Static Timing Analysis (STA) tools. In order to reduce the transistor inventory that needs to be tracked by the STA solver, we develop a threshold-pruning methodology to identify the variation-critical part of a design. That way, we accelerate variation-aware STA iterations, with a maximum speedup of 6.82x achieved for representative benchmark circuits. We substantiate the efficiency of the proposed framework for realistic designs. For a CPU datapath, our threshold-pruning technique outperforms built-in pruning commands of the STA solver by 16.87% in terms of runtime improvement. We demonstrate the impact of BTI after three years of operation, with clock frequency degradation up to 24% and functional yield reduction below 90% for higher frequencies.
Intestinal parasites in patients with diarrhea and human immunodeficiency virus infection in Zimbabwe (1999)

E. bieneusi infection was common in HIV-infected patients with diarrhea in Zimbabwe and may be acquired through person-to-person and fecal-oral transmission.