gebhart/patrepeval huggingface dataset:
Figure S3 from CD103<sup>+</sup> Tumor-Resident CD8<sup>+</sup> T Cells Are Associated with Improved Survival in Immunotherapy-Naïve Melanoma Patients and Expand Significantly During Anti–PD-1 Treatment (2023)

&lt;p&gt;Supplementary data on gating strategy used in the study&lt;/p&gt;
A method towards simulating the total luminous flux of a monochromatic high power LED operated in a pulsed manner (2009)

High power light-emitting diodes (HPLEDs) are frequently being operated in a pulsed manner. The research presented here focuses on the optical, electrical and thermal behaviour of a HPLED under pulse width modulation (PWM), and has the following twofold aim. Firstly, investigating the temperature dependence of the HPLED's efficiency, where it was found that the exact method of operation and the definition of calculation is crucial when making claims. Secondly, we propose a method to simulate the absolute emitted luminous flux of a current driven HPLED under PWM. This is done by making use of experimentally determined characteristic parameters of the HPLED. This has as advantage that no further physical measurements are needed to investigate the HPLEDs behavior under numerous different PWM circumstances.
Extremely uniform lasing wavelengths of InP microdisk lasers heterogeneously integrated on SOI (2013)

A standard deviation in lasing wavelength lower than 500pm is characterized on nominally identical and optically-pumped microdisk lasers, heterogeneously integrated on the same SOI circuit. This lasing wavelength uniformity is obtained using electron-beam lithography.
Pivotal Phase 3 Randomized Clinical Trial of the Safety, Tolerability, and Immunogenicity of 20-Valent Pneumococcal Conjugate Vaccine in Adults Aged ≥18 Years (2021)

NCT03760146.
[Preliminary studies on the characterization of adenylate cyclase from segmental ganglia of the leech Hirudo medicinalis]. (1981)

Adenylate cyclase activity has been measured in segmental ganglia of the leech Hirudo m. Basal enzymatic activity was stimulated in a dose dependent fashion by serotonin. GTP is required in order to evoke maximal stimulation. The effect was blocked by methysergide, an antagonist of serotonin at receptor level. The stimulation of the enzyme was prevented by alpha beta-methy-len-ATP. In addition RMI 12330A inhibited basal as well as serotonin-stimulated adenylate cyclase activity presumably by acting at the catalytic subunit level.
Controlling the Integration of Emotion and Cognition (2006)

Emotion has been both lauded and vilified for its role in decision making. How are people able to ensure that helpful emotions guide decision making and irrelevant emotions are kept out of decision making? The orbitofrontal cortex has been identified as a neural area involved in incorporating emotion into decision making. Is this area's function specific to the integration of emotion and cognition, or does it more broadly govern whether emotional information should be integrated into cognition? The present research examined the role of orbitofrontal cortex when it was appropriate to control (i.e., prevent) the influence of emotion in decision making (Experiment 1) and to incorporate the influence of emotion in decision making (Experiment 2). Together, the two studies suggest that activity in lateral orbitofrontal cortex is associated with evaluating the contextual relevance of emotional information for decision making.
Desialylation of insulin receptors and IGF-1 receptors by neuraminidase-1 controls the net proliferative response of L6 myoblasts to insulin (2010)

We recently established that the subunit of cell surface-residing elastin receptor, neuraminidase-1 (Neu1), can desialylate adjacent insulin-like growth factor 1 receptors (IGF-1R) of arterial smooth muscle cells, thereby quenching their proliferative response to insulin-like growth factor II. In this study, we explored whether Neu1 would also desialylate the insulin receptors (IR), as well as the IGF-1R on rat skeletal L6 myoblasts, and whether desialylation of IR and IGF-1R would affect a net proliferative effect of insulin. First, we found that physiological (0.5-1 nM) and high therapeutic (10 nM) insulin concentrations induced a modest increase in proliferation rate of cultured L6 myoblasts. While IR kinase inhibitor could abolish the mitogenic effect of these insulin concentrations, the observed more pronounced proliferative response to supraphysiological concentration (100 nM) of insulin could be eliminated only by specific inhibition of IGF-1R. Then, we found that treatment of L6 cells with mouse-derived Neu1 or with Clostridium perfringens neuraminidase caused desialylation of IR, which coincided with a significant increase of their proliferative response to lower (0.5-10 nM) concentrations of insulin. In contrast, experimental desialylation of IGF-1R coincided with elimination of the heightened proliferative response of L6 myoblasts to 100 nM insulin. Importantly, we also found that inhibition of endogenous Neu1 abolished the increase in proliferation of L6 cells induced by 1 and 10 nM of insulin, but amplified the proliferative effect of 100 nM insulin. We therefore conclude that desialylation of both IR and IGF-1R by Neu1 controls the net proliferative response of skeletal myoblasts to insulin.
The experiences of adults with intellectual disabilities and their carers in general hospitals: a focus group study (2008)

The experiences of participants in this study concur with and add to concern expressed in recent reports and published research. Wide ranging implications are discussed for further research, wider policy development, clinical practice, local health service provision and education of health professionals.
Functionalized magnetonanoparticles for MRI diagnosis and localization in epilepsy (2008)

Nonradioactive AMT-MNP can cross the BBB and may accurately localize epileptogenic cerebral regions. The MNP-MRI approach is potentially applicable to the use of any bioactive molecules as ligands for imaging normal and abnormal localized cerebral functions, accurately, safely, and inexpensively.
Selexipag Active Metabolite ACT-333679 Displays Strong Anticontractile and Antiremodeling Effects but Low β-Arrestin Recruitment and Desensitization Potential (2017)

Prostacyclin (PGI<sub>2</sub>) receptor (IP receptor) agonists, which are indicated for the treatment of pulmonary arterial hypertension (PAH), increase cytosolic cAMP levels and thereby inhibit pulmonary vasoconstriction, pulmonary arterial smooth muscle cell (PASMC) proliferation, and extracellular matrix synthesis. Selexipag (Uptravi, 2-{4-[(5,6-diphenylpyrazin-2-yl)(isopropyl)amino]butoxy}-<i>N</i>-(methylsulfonyl)acetamide) is the first nonprostanoid IP receptor agonist, it is available orally and was recently approved for the treatment of PAH. In this study we show that the active metabolite of selexipag and the main contributor to clinical efficacy ACT-333679 (previously known as MRE-269) behaved as a full agonist in multiple PAH-relevant receptor-distal-or downstream-cellular assays with a maximal efficacy (<i>E</i><sub>max</sub>) comparable to that of the prototypic PGI<sub>2</sub> analog iloprost. In PASMC, ACT-333679 potently induced cellular relaxation (EC<sub>50</sub> 4.3 nM) and inhibited cell proliferation (IC<sub>50</sub> 4.0 nM) as well as extracellular matrix synthesis (IC<sub>50</sub> 8.3 nM). In contrast, ACT-333679 displayed partial agonism in receptor-proximal-or upstream-cAMP accumulation assays (<i>E</i><sub>max</sub> 56%) when compared with iloprost and the PGI<sub>2</sub> analogs beraprost and treprostinil (<i>E</i><sub>max</sub> ∼100%). Partial agonism of ACT-333679 also resulted in limited <i>β</i>-arrestin recruitment (<i>E</i><sub>max</sub> 40%) and lack of sustained IP receptor internalization, whereas all tested PGI<sub>2</sub> analogs behaved as full agonists in these desensitization-related assays. In line with these in vitro findings, selexipag, but not treprostinil, displayed sustained efficacy in rat models of pulmonary and systemic hypertension. Thus, the partial agonism of ACT-333679 allows for full efficacy in amplified receptor-distal PAH-relevant readouts while causing limited activity in desensitization-related receptor-proximal readouts.
Estimating Conductivity and Transference Numbers of Concentrated ZnCl2 / KCl Electrolytes (1991)

Solution conductivities and transference numbers of concentrated solutions are estimated by describing ionic movement in a hypothetical Hittorf cell. Mass transfer is based on dilute solution assumptions as well as accounting for complexation and hydration of the ionic species. Such a simple representation of mass transport predicts solution conductivity and formal transference numbers of zinc in reasonable agreement with experimental data, including negative transference numbers indicative of the dominant effect of negatively charged zinc halocomplexes.
Genetic Approaches to Polyketide Antibiotics. 1 (2005)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTGenetic Approaches to Polyketide Antibiotics. 1Robert McDaniel, Mark Welch, and C. Richard HutchinsonView Author Information Kosan Biosciences, 3832 Bay Center Place, Hayward, California 94545 Cite this: Chem. Rev. 2005, 105, 2, 543–558Publication Date (Web):January 25, 2005Publication History Received18 June 2004Published online25 January 2005Published inissue 1 February 2005https://pubs.acs.org/doi/10.1021/cr0301189https://doi.org/10.1021/cr0301189research-articleACS PublicationsCopyright © 2005 American Chemical SocietyRequest reuse permissionsArticle Views1943Altmetric-Citations101LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Cluster chemistry,Genetics,Lactones,Metabolism,Peptides and proteins Get e-Alerts
Systematically Controlling Acceptor Fluorination Optimizes Hierarchical Morphology, Vertical Phase Separation, and Efficiency in Non‐Fullerene Organic Solar Cells (2021)

Abstract Non‐fullerene acceptor (NFA) end group (EG) functionalization, especially by fluorination, affects not only the energetics but also the morphology of bulk‐heterojunction (BHJ) organic solar cell (OSC) active layers, thereby influencing the power conversion efficiency (PCE) and other metrics of NFA‐based OSCs. However, a quantitative understanding of how varying the degrees of NFA fluorination influence the blend morphological and photovoltaic properties remains elusive. Here a series of three A‐DAD‐A type NFAs (D = π‐donor group and A = π‐acceptor EG) which systematically increase the degree of EG fluorination and comprehensively investigate the resulting blends with the polymer donor PM6 in terms of optical properties, electronic structure, film crystallinity, charge carrier transport, and OSC performance is reported. The results indicate that the most highly fluorinated NFA, BT‐BO‐L4F, achieves an optimal BHJ hierarchical morphology where enhanced NFA molecule intermolecular π–π stacking and optimal vertical phase gradation are achieved in the BHJ blend. These factors also promote optimum NFA‐cathode contact, more balanced electron and hole mobility, and suppress both monomolecular and bimolecular recombination. As a result, both the short‐circuit current density and fill factor in this OSC series progressively increase with increasing EG fluorine density, and the resulting PCEs increase from 9 to 16.8%.
Non-persuasive communication as a strategy to deliver climate information&#160; (2022)

&amp;lt;p&amp;gt;The Monash Climate Change Communication Research Hub (MCCCRH) is Australia&amp;amp;#8217;s leading research centre dedicated to improving public understanding of climate change.&amp;amp;#160;&amp;lt;/p&amp;gt;&amp;lt;p&amp;gt;Utilising what is known as &amp;amp;#8216;non-persuasive communication,&amp;amp;#8217; MCCCRH delivers simple, short, factual climate messages over longer-time frames, that are repeated often. The approach is large scale, evidence-based and non-partisan and is delivering the information that Australians are seeking to actively bridge deep divisions on climate change.&amp;lt;/p&amp;gt;&amp;lt;p&amp;gt;In this paper we present non-persuasive communication in action that is done by translating climate science into simple and engaging formats for two of MCCCRH&amp;amp;#8217;s major programs:&amp;lt;/p&amp;gt;&amp;lt;ul&amp;gt;&amp;lt;li&amp;gt;&amp;amp;#8216;Climate Communicators&amp;amp;#8217;, which is an innovative national climate education program that produces simple, long-term climate graphics for Australian television weather presenters and currently broadcasts in every state of Australia with more than 500+ broadcasts to date. It broadcasts to metropolitan and rural television audiences and harnesses the existing science communication capacity of weather presenters.&amp;lt;/li&amp;gt; &amp;lt;/ul&amp;gt;&amp;lt;p&amp;gt;&amp;amp;#160;&amp;lt;/p&amp;gt;&amp;lt;ul&amp;gt;&amp;lt;li&amp;gt;&amp;amp;#8216;Changing Climates&amp;amp;#8217;, which is a climate column in collaboration with Australia&amp;amp;#8217;s largest media organisation News Corp Australia that increases the visibility of climate science and scientists and has published 2000+ print and digital columns across 58 mastheads.&amp;amp;#160;&amp;lt;/li&amp;gt; &amp;lt;/ul&amp;gt;&amp;lt;p&amp;gt;Most up to date and accurate climate science and data is brokered from the Bureau of Meteorology, CSIRO and Monash University scientists. Easy-to-process data visualisations are continuously researched with leading science communications specialists to ensure clarity and shared with partners from the most visible and trusted forms of media including television weather presenters and community newspapers. The effectiveness of these strategies is routinely evaluated, ensuring their design and delivery are specifically tailored for maximum impact.&amp;amp;#160;&amp;lt;/p&amp;gt;&amp;lt;p&amp;gt;&amp;amp;#160;&amp;lt;/p&amp;gt;&amp;lt;p&amp;gt;Corresponding/presenting author: David Hoffmann, Monash University, Melbourne, Australia; david.hoffmann@monash.edu&amp;lt;/p&amp;gt;&amp;lt;p&amp;gt;&amp;amp;#160;&amp;lt;/p&amp;gt;
Expression of membrane Hsp90 is a molecular signature of T cell activation (2022)

Heat shock protein 90 (Hsp90) maintains cellular proteostasis during stress and has been under investigation as a therapeutic target in cancer for over two decades. We and others have identified a membrane expressed form of Hsp90 (mHsp90) that previously appeared to be restricted to rapidly proliferating cells exhibiting a metastatic phenotype. Here, we used HS-131, a fluor-tethered mHsp90 inhibitor, to quantify the effect of T cell activation on the expression of mHsp90 in human and mouse T cells. In cell-based assays, stimulation of human T cells induced a 20-fold increase in mHsp90 expression at the plasma membrane, suggesting trafficking of mHsp90 is regulated by TCR and inflammatory mediated signaling. Following injection of HS-131 in mouse models of human rheumatoid arthritis and inflammatory bowel disease, we detected localization of the probe at sites of active disease, consistent with immune cell invasion. Moreover, despite rapid hepatobiliary clearance, HS-131 demonstrated efficacy in reducing the mean clinical score in the CIA arthritis model. Our results suggest mHsp90 expression on T cells is a molecular marker of T cell activation and potentially a therapeutic target for chronic diseases such as rheumatoid arthritis.
Glass‐transition temperature in the curing process of bismaleimide modified with diallylbisphenol A (2004)

Abstract The T g ‐conversion relationship, during the thermal curing of different stoichiometric formulations of 1,1′‐(methylene‐di‐4,1‐phenylene) bismaleimide (BMI), modified with o , o ′‐diallyl bisphenol A (DABA), was investigated. The DiBenedetto equation was used to model this relationship for the formulation of DABA‐1 (BMI : DABA, 1 : 1). Based on this model, the T g ‐conversion relationship of formulation DABA‐0.5 (BMI : DABA, 1 : 0.5) was modeled. The high consistency between the model curve and experimental data showed that the change of T g , attributed to copolymerization between BMI and DABA in DABA‐0.5, in the low‐conversion regime, was the same as that in DABA‐1. This also verifies that, for the formulation DABA‐0.5, copolymerization and homopolymerization do not overlap with each other. The reactions progressed sequentially and homopolymerization occurred after completion of copolymerization. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 91: 3244–3247, 2004
Structural Characterization of Thick GaN Films Grown on Free-Standing GaN Seeds by the Ammonothermal Method Using Basic Ammonia (2005)

Single crystalline GaN was grown on c -plane free-standing GaN seeds via fluid transport by the ammonothermal method. Ammonothermal growth was carried out in an ammonobasic solution with a metallic Ga nutrient placed in a lower-temperature zone. The thickest film obtained so far is ∼45 µm, which was grown on the N-face of the seed. The growth interface contained numerous voids and defects, whereas the microstructure close to the surface was greatly improved. The major defects close to the surface were mixed-character threading dislocations. The estimated threading dislocation density was low-10 9 cm -2 level at the free surface of the N-face.
On Correlating Experimental Pressure Flow and Heat Transfer Measurements From Silicon Microchannels With Theoretical Calculations (2007)

The bulk pressure flow and heat transfer characteristics of rectangular and trapezoidal microchannels etched in silicon were measured in the laminar regime. The channel hydraulic diameters were 305 μm for the Deep Reactive Ion Etched (DRIE) etched channel and 317 μm for the wet etched channel and there were 22 channels in each sample. The fluid used was purified degassed water. The inlet and outlet temperature and pressure of the fluid and the wall temperatures of the channels were measured at the inlet and outlet of the channels. Theoretical and experimental results were calculated using fluid properties at the mean fluid temperature for each data point. These were then collapsed to a single curve at constant temperature by multiplying the measured value by the ratio of the relevant fluid properties at the experimental and required temperatures. The cross section of each channel on each channel sample was measured along with the channel height and width to give an area ratio between the actual channel width and the width calculated assuming the channel was perfectly rectangular or trapezoidal. This ratio is used to compensate the theoretical results and improve their correlation with the experiment. The uncertainty in the experimental results was calculated by running the result processing calculations three times, once at nominal values and then shifting input values to their upper and lower limits based on a 95% confidence interval on the standard deviation for each inputted measurement. Theoretical calculations were run for each experimental mass flow rate in order to produce equivalent theoretical points to the experimental values. Uncertainty in the theory is also determined by running the theoretical calculations at upper, lower and nominal 95% confidence interval values for the channels being tested. It was found that while the pressure flow data from the channels matched theoretical trends and that the results for the rectangular DRIE channels showed no experimentally significant deviation from theory, the experimental data from the wet etched trapezoidal channels was lower than predictions. The heat transfer from the channels is strongly affected by the heat transferred to the coolant by the manifolds. When this effect is removed, the experimental Reynolds number Nusselt number plot becomes strongly linear. This does not agree with theoretical predictions.
Development of a Novel Method for in Vitro Analysis of CD8 Thymocyte Selection and Maturation, (2011)

Abstract Abstract 3235 T cell development relies on the interaction between the T-cell receptor (TCR) on thymocytes and the self-major histocompatibility complex (MHC) expressed on thymic epithelial cells in the thymus. This process, called positive selection, rescues developing thymocytes from cell death while leading to their differentiation into mature T cells. Since it is believed that the proper development of CD8 T cells requires an intact thymus, several groups studied their development using fetal or reaggregation thymus organ cultures in vitro. Unfortunately, these models were shown to be cumbersome requiring a complicated set-up while generating limited cellular yield. Thus, we sought of developing a novel in vitro system using bone marrow-derived stromal cells to support CD8 T cell development and maturation in vitro. We selected the OTI system as a working model due to the availability of previously identified positively selecting peptides. Non-selected T-cell-committed double-positive (DP) OTI thymocytes (CD4+CD8+CD69−) were first fractionated based on the surface expression intensity of both TCR and CD5. These 3 subsets designated as TCRloCD5lo (DP1), TCRintCD5hi (DP2), and TCRhiCD5int (DP3) express different levels of ZAP70. Following fractionation, the DP subsets were co-cultured with bone marrow-derived stromal cells presenting OTI-selecting peptides. In the absence of cytokines, no CD8+ OTI cell development occurred in vitro. When repeated in the presence of γc-cytokines (IL2, IL4, IL7, IL9, IL15 and IL21) only rIL4 and rIL7 were able to induce CD8 T cell development. Supplementing the co-culture system with rIL4 led to the generation of 50–60% single-positive (SP) CD8 T cells only from the DP3 fraction whereas rIL7 induced the development of a minor fraction of CD8 T cells from DP2s (3–4%) and a major population from DP3 (50–76%). Furthermore, we found that rIL4 treatment triggers the development of 2 distinct populations of SP OTI cells (based on their CD8 expression intensity) which we termed CD8int and CD8hi. When analyzed by flow-cytometry, ex vivo generated CD8int, but not CD8hi, expressed high levels of CD69, PD-L1 and CD44. In contrast, SP CD8 T cells developed in the presence of rIL7 did not upregulate these markers. Since IL7 promotes survival and proliferation of TCR-triggered DPs while IL4 affects their differentiation, we admixed both cytokines during the co-culture and found a dominant rIL4 effect: the phenotype of SP CD8 T cells was similar to that induced by rIL4 alone. Taken together, our findings demonstrate that some DP thymocytes are efficiently selected in our system by OTI-specific positively selecting peptides. Notably, the addition of rIL7 leads to the development and maturation of classic CD8 T cells whereas rIL4 induces both classic and innate CD8 T cells. This work was supported by grant a from CIHR. Disclosures: No relevant conflicts of interest to declare.