gebhart/patrepeval huggingface dataset:
Stable expression of truncated inositol 1,4,5-trisphosphate receptor subunits in 3T3 fibroblasts. Coordinate signaling changes and differential suppression of cell growth and transformation. (1994)

Stable clones of NIH 3T3 fibroblasts transfected with the cDNA of either the wild-type or deletion forms of the rat type I (or cerebellar) inositol 1,4,5-trisphosphate (IP3) receptor (IP3R) were investigated. The delta form, missing the NH2-terminal sequence that includes the IP3-binding site, is expected to be still assembled with wild-type subunits to yield a tetrameric Ca2+ channel across the endoplasmic reticulum membrane; the s form, missing the membrane-spanning sequences, is expected to remain as a soluble monomer in the cytosol. With respect to control clones transfected with the vector only, the synthesis fo IP3Rs was markedly stimulated in the receptor-transfected clones. The mass accumulation, however, was increased only moderately (deletion forms = 15-30% of the endogenous IP3R), apparently because of a compensatory increase in receptor turnover. Coordinate changes in IP3 generation and Ca2+ release were revealed in the delta clones by experiments in both intact and permeabilized cells. In these clones, the IP3R was more sensitive to IP3, and IP3 generation at the ATP P2u surface receptor was decreased. This latter effect was due neither to a defect in G protein coupling nor to changes in phospholipase C expression, but to down-regulation of the P2u receptor. In the cells expressing the s- and delta-IP3R subunits, no differences with respect to the controls were observed in epidermal growth factor-induced DNA synthesis, whereas long-term growth stimulated by serum was reduced. Even more marked, especially in the delta clones (-90%), was the inhibition of cell transformation induced by autocrine stimulation with transforming growth factor alpha of the overexpressed epidermal growth factor receptors or by other growth factor receptors and oncogenes (platelet-derived growth factor/platelet-derived growth factor receptor beta, HER2/neu, and v-erbB). These effects appear not to be connected to the signaling processes mediated by tyrosine phosphorylation since the latter was unchanged in the delta clones. These results demonstrate for the first time (a) that the changes in cellular homeostasis directly induced by deleted IP3R subunits (increased receptor synthesis and increased IP3R sensitivity) are largely compensated by indirect coordinate changes apparently aimed to keep near normal the signaling properties of the cells; (b) that modulation of intracellular Ca2+ channels induces profound consequences that differentially affect growth and oncogenesis; and (c) that IP3Rs and the Ca2+ stores are important cross-roads of intracellular signaling pathways.
Clinical and angiographic results with the NIR stent: First International NIR Endovascular Stent Study (FINESS-II) (2000)

BACKGROUND: Although safety and efficacy of the NIR trade mark stent have been reported, the long-term angiographic and clinical outcomes have yet to be investigated. The FINESS-II study (First International NIR Endovascular Stent Study) was designed to assess the procedural safety of single 9 and 16 mm NIR stent implantation, the six-month restenosis rate and finally the six- and 12-month clinical outcome of patients treated with this novel coronary stent. METHODS: Patients with angina and a single de novo lesion in a native coronary artery of >3 and <5 mm diameter were included in this multicentre, prospective, observational trial. Clinical follow-up was obtained at one, six and 12 months. Angiography was performed before and after the stent implantation and at six months. The primary endpoint included major adverse cardiac events (death, myocardial infarction and target lesion revascularization) within 30 days after the procedure. Major bleeding complications and subacute stent thrombosis within the first 30 days were also reported as specific endpoints. Secondary endpoints were major cardiac-event-free survival at six- and 12-month follow-up and angiographic restenosis at six months. RESULTS: A total of 156 patients (81% male, mean age 60 +/- 10 years), with stable (54%), unstable (40%) angina pectoris or silent ischemia (6%) were enrolled. The target vessel diameter was 2.94 +/- 0.54 mm. The minimal lumen diameter pre, post and at follow-up was 1.04 +/- 0.32 mm, 2.64 +/- 0.42 mm and 1.88 +/- 0.63 mm, respectively. Restenosis rate according to the >50% diameter stenosis criterion at six month follow-up was 19% (26/136). At 12 months, the event-free survival rate was 83% (two deaths, one Q-wave and three non-Q-wave myocardial infarctions, four bypass surgery and 17 target lesion revascularizations), while 87% of the patients were free of angina pectoris. CONCLUSION: the outcome of the FINESS-II trial is comparable to those observed in previous stent trials (Benestent II), indicating that the coronary NIR stent is safe and effective as a primary device for the treatment of native coronary artery lesions in patients with (un)stable angina pectoris.
An asymmetric, dynamic, energy-conserving ARQ protocol (2003)

Mobile computing implies reliance on a portable, self-contained power source and increased difficulty of communications due to the highly-variable radio channel. In order to ensure reliable, verified, error-free delivery of data in the presence of multipath fading, automatic repeat request (ARQ) schemes are used to synchronize and acknowledge the transmission of data between the base station and the mobile node. We build upon previous designs of energy-conserving variants of the classical selective repeat protocol to design a dynamic, asymmetric energy-conserving protocol. Primarily, in this work, we move the complexity of the protocol to the base station, which can optimally assign on a packet-by-packet basis the set of energy-conserving ARQ protocol and parameters which is best suited to the current radio channel, traffic and energy requirements. In this manner, the best delay vs. energy trade-off of previously proposed protocols can be incorporated in a practical, dynamic way. Through simulation we evaluate the average packet delay of different classes of service under strict energy constraints and varying channel characteristics. The results derived here show the significant advantage of quickly adapting the protocol to changing channel fading characteristics so as to maintain a required energy vs. delay trade-off between multiple priority classes of traffic.
A novel tumor suppressing gene, ARHGAP9, is an independent prognostic biomarker for bladder cancer (2019)

Screening for genes or markers relevant to bladder cancer (BC) tumorigenesis and progression is of vital clinical significance. The present study used reverse-transcription quantitative PCR reaction assays to examine the expression of mRNA encoding Rho GTPase-activating protein 9 (ARHGAP9) in BC tissue samples and to determine whether <i>ARHGAP9</i> is an independent prognostic biomarker for non-muscle invasive BC (NMIBC) and muscle invasive BC (MIBC). The results revealed that the downregulation of <i>ARHGAP9</i> expression in the tissue of patients with NMIBC or MIBC was significantly associated with a poor prognosis. In patients with NMIBC, a high expression of <i>ARHGAP9</i> was significantly associated with prolonged recurrence-free survival, whereas in MIBC patients, it was significantly associated with an increased progression-free and cancer-specific survival. The risk of cancer-specific death was 2.923 times higher (95% confidence interval, 1.192-7.163) when <i>ARHGAP9</i> levels were decreased. In conclusion, lower expressions of <i>ARHGAP9</i> correlated with BC prognosis, indicating that it may be a useful marker for guiding treatment application.
Osteoprotective Activity and Metabolite Fingerprint via UPLC/MS and GC/MS of Lepidium sativum in Ovariectomized Rats (2020)

<i>Lepidium sativum</i> seeds are used traditionally to accelerate healing of bone fracture in addition to its culinary uses. This study aimed to characterize the osteoprotective effect of <i>L. sativum</i> in an ovariectomized rat model at two dose levels (50 and 100 mg/kg) using 17β-estradiol as a positive reference standard. Moreover, a complete metabolite profile of <i>L. sativum</i> via UHPLC/PDA/ESI-MS, as well as headspace solid-phase microextraction (SPME)-GC/MS is presented. Results revealed that <i>L. sativum</i> extract exhibited significant anti-osteoporotic actions as evidenced by mitigating the decrease in relative bone weight concurrent with improved longitudinal and perpendicular femur compression strength. Further, the extract enhanced the serum bone formation biomarkers lactate dehydrogenase (LDH) activity and osteocalcin levels. The extract also inhibited exhaustion of superoxide dismutase (SOD) as well as glutathione peroxidase (GPx) activities and accumulation of lipid peroxides in bone tissues. This is in addition to ameliorating the rise in the markers of bone resorption carboxyterminal telopeptide, type I (CTXI) and tartrate-resistant acid phosphatase (TRAP) and modulating receptor activator of nuclear factor kappa-Β ligand (<i>RANKL</i>)/ osteoprotegerin (<i>OPG</i>) expression. Metabolite characterization suggests that glucosinolates, lignans, coumarins, phenolic acids, and alkaloids mediate these anti-osteoporotic effects in a synergistic manner.
Chalcogen Bonds in Selenocysteine Seleninic Acid, a Functional GPx Constituent, and in Other Seleninic or Sulfinic Acid Derivatives (2021)

The controlled oxidation reaction of L-selenocystine under neutral pH conditions affords selenocysteine seleninic acid (3-selenino-L-alanine) which is characterized also by means of single-crystal X-ray diffraction. This technique shows that selenium forms three chalcogen bonds (ChBs), one of them being outstandingly short. A survey of seleninic acid derivatives in the Cambridge Structural Database (CSD) confirms that the C-Se(=O)O- functionality tends to act as a ChB donor robust enough to systematically influence the interactional landscape in the solid. Quantum Theory of Atom in Molecules (QTAIM) analysis proves the attractive nature of the short contacts observed in crystals containing the seleninic functionality and calculation of surface molecular electrostatic potential (MEP) reveals that remarkably positive σ-holes can frequently be found opposite to the covalent bonds at selenium. Both CSD searches and QTAIM and MEP approaches show that also the sulfinic acid moiety can function as a ChB donor, albeit less frequently than the seleninic acid one. These findings may contribute to a better understanding, at the atomic level, of the mechanism of action of the enzymes that control oxidative stress and ROS deactivation and that contain selenocysteine seleninic acid and cysteine sulfinic acid in the active site.
The use of metamodeling for VoIP over WiFi capacity evaluation [Transactions Letters] (2008)

The increasing popularity of wireless fidelity (Wi-Fi) networks at home, in public areas and in the enterprise motivates extensive modeling and analysis of their performance measures, such as network capacity and quality of service (QoS) capabilities. Some of the easier performance problems can be solved by analytical modeling methods, but most of the complicated ones, involving several design parameters from multiple layers, can only be answered through simulation studies that are typically implicit and less suitable for design and optimization. Hence, we believe it is crucial to obtain explicit mathematic models, that are effective in representing system behavior and a basis for performance optimization. Here, we first advocate the application of formal empirical modeling techniques to performance studies of Wi-Fi networks, in order to find usable, if approximate, closed-form mathematical models. Subsequently, by applying these metamodeling techniques, we perform a case study with very useful results: our VoWiFi (voice over Wi-Fi) admission capacity metamodel gives a much tighter bound than those existing in the literature and leads to a more effective admission control scheme. Our work, therefore, points out a new direction for future performance studies of Wi-Fi networks.
Electrically Controlled Plasmonic Behavior of Gold Nanocube@Polyaniline Nanostructures: Transparent Plasmonic Aggregates (2016)

We demonstrate the electrically controlled and reversible plasmonic signature of hybrid polymer–metal nanostructures composed of core/shell nanostructures: gold nanocubes (AuNCs) coated with electrochromic polyaniline (PANI) shells. A reversible tuning of the localized surface plasmon resonance (LSPR) peak of the AuNC core was obtained by applying an electrical potential that caused a reversible oxidation state change in the electroactive PANI nanoshell. A significant shift of the main LSPR peak was achieved with high reversibility and electrochemical stability due to the interplay of the local decay of the electromagnetic field and the controlled thickness of the surrounding polymer shell. Here, the PANI shell acts as an electroactive medium as well as a physical spacer to prevent uncontrollable plasmonic coupling. The most efficient LSPR shift can be induced by the refractive index change of nanoscale PANI shell thickness lower than the electromagnetic field decay length of the given gold nanoparticles. Single particle studies showed that coupling of plasmon resonances of densely packed nanocubes with thicker PANI shells was prevented and the extinction signature of individual core/shell nanocubes remained mostly unchanged after their assembly into densely packed aggregates. Therefore, these core/shell structures could preserve the original plasmonic signature of individual nanostructure by damping plasmonic coupling between AuNC cores.
The double-directional radio channel (2001)

We introduce the concept of the double-directional mobile radio channel. It is called this because it includes angular information at both link ends, e.g., at the base station and at the mobile station. We show that this angular information can be obtained with synchronized antenna arrays at both link ends. In wideband high-resolution measurements, we use a switched linear array at the receiver and a virtual-cross array at the transmitter. We evaluate the raw measurement data with a technique that alternately used estimation and beamforming, and that relied on ESPRIT (estimation of signal parameters via rotational invariance techniques) to obtain superresolution in both angular domains and in the delay domain. In sample microcellular scenarios (open and closed courtyard, line-of-sight and obstructed line-of-sight), up to 50 individual propagation paths are determined. The major multipath components are matched precisely to the physical environment by geometrical considerations. Up to three reflection/scattering points per propagation path are identified and localized, lending insight into the multipath spreading properties in a microcell. The extracted multipath parameters allow unambiguous scatterer identification and channel characterization, independently of a specific antenna, its configuration (single/array), and its pattern. The measurement results demonstrate a considerable amount of power being carried via multiply reflected components, thus suggesting revisiting the popular single-bounce propagation models. It turns out that the wideband double-directional evaluation is a most complete method for separating multipath components. Due to its excellent spatial resolution, the double-directional concept provides accurate estimates of the channel's multipath-richness, which is the important parameter for the capacity of multiple-input multiple-output (MIMO) channels.
Behaviour of osteoblast-like cells in cellular microdevices (2005)

Times Cited: 0 Leclerc, E David, B Warocquier-Clerout, R Griscom, L Le Pioufle, B Fujii, T Legallais, C 8th International Conference on Miniaturized Systems for Chemistry and Life Sciences Sep 26-30, 2004 Malmo, SWEDEN VINNIVA, Crafoord Fdn, Swedish Fdn Strateg Res, MCPT, Micralyne, SAMSUNG, Micro Plastab, GYROS, Lung Univ, Erysave AB, ATS Med, Silex Microsyst, Johnson &Johnson, Caliper Life Sci, MicroFluid Syst Inc, Analyt Chem, Sci AAAS, Lab Chip, Journal Separat Sci
NONLINEAR MEDIA WITH TWO-DIMENSIONAL FEEDBACK (1988)

Optical bistability, instabilities, and chaos are usually observed as processes developing with time. (The only exception is the so-called transverse optical bistability.) The three-dimensional nature of optical fields enables us to propose radically new nonlinear phenomena in systems with suitably organized feedback. In this paper a new class of nonlinear optical systems is presented and experimentally tested - coherent systems with two-dimensonal feed­ back. We discuss the conditions of initiation of spatial instabilities, spatial bistability, optical self-organizing processes, and optical turbulence, which develops in space and time like hydrodynamic turbulence. These phenomena were oberved in a hybrid system with a liquid crystal. New possibilities of applications in physics and optical computing provided by systems with two-dimensional feedback are noted.
Analysis of pH Dependent Uranium(VI) Sorption to Nanoparticulate Hematite by Flow Field-Flow Fractionation - Inductively Coupled Plasma Mass Spectrometry (2009)

The ability to quantify the amount of metals ions that are present as macromolecular, nanoparticulate, or colloid phases is critical for understanding bioavailability and transport as well as performing risk assessments and remediation strategies. Flow field-flow fractionation-inductively coupled plasma mass spectrometry (FI FFF-ICP-MS) is a powerful separation tool that has been previously used to characterize colloidal metals in environmental samples. In this study we examine the degree to which FI FFF-ICP-MS provides quantitative data on uranium speciation by comparing the results to centrifugation followed by filtration. Sorption of uranium to nanoparticulate hematite (approximately 60 nm) was examined over the pH range of 3 to 6. Close agreement was found between the two approaches over the pH range.
Germanium oxynitride gate dielectrics formed by plasma nitridation of ultrathin thermal oxides on Ge(100) (2009)

Germanium oxynitride (GeON) gate dielectrics with surface nitrogen-rich layers were fabricated by plasma nitridation of thermally grown oxides (GeO2) on Ge(100). Insulating features of ultrathin GeO2 layers of around 2-nm-thick were found to improve with plasma treatment, in which leakage current was drastically reduced to over four orders of magnitude. Consequently, Au/GeON/Ge capacitors of an equivalent oxide thickness down to 1.7 nm were achieved while keeping sufficient leakage reduction merit. The minimum interface state density values of GeON/Ge structures as low as 3×1011 cm−2 eV−1 were obtained for both the lower and upper halves of the bandgap without any postnitridation treatments. These results were discussed based on the effects of plasma nitridation on a degraded GeO2 surface for recovering its electrical properties by creating stable nitride layers.
Data from Role of placenta growth factor in malignancy and evidence that an antagonistic PlGF/Flt-1 peptide inhibits the growth and metastasis of human breast cancer xenografts (2023)

&lt;div&gt;Abstract&lt;p&gt;The angiogenic growth factor placenta growth factor (PlGF) is implicated in several pathologic processes, including the growth and spread of cancer. We found by immunohistochemistry that 36% to 60% and 65% of primary breast cancers express PlGF and its receptor Flt-1, respectively. These findings suggest that PlGF may be active in tumor growth and metastasis beyond its role in angiogenesis. It was found that exogenously added PlGF (2 nmol/L), in contrast to vascular endothelial growth factor (2 nmol/L), significantly stimulated &lt;i&gt;in vitro&lt;/i&gt; motility and invasion of the human breast tumor lines MCF-7 and MDA-MB-231. A PlGF-2/Flt-1–inhibiting peptide, binding peptide 1 (BP1), that binds Flt-1 at or near the heparin-binding site was identified and synthesized. Both PlGF-stimulated motility and invasion were prevented by treatment with BP1 (&lt;i&gt;P&lt;/i&gt; &lt; 0.05), as well as by anti-PlGF antibody. Treatment of mice bearing s.c. MDA-MB-231 with BP1 (200 μg i.p., twice per week) decreased the number of spontaneous metastatic lung nodules by 94% (&lt;i&gt;P&lt;/i&gt; &lt; 0.02), whereas therapy of animals with orthotopic mammary fat pad tumors decreased pulmonary metastases by 82% (&lt;i&gt;P&lt;/i&gt; &lt; 0.02). These results indicate, for the first time, that PlGF stimulates the metastatic phenotype in these breast cancer cells, whereas therapy with a PlGF-2/Flt-1 heparin-blocking peptide reduces the growth and metastasis of human breast cancer xenografts. [Mol Cancer Ther 2007;6(2):524–31]&lt;/p&gt;&lt;/div&gt;
Die katalytische Reduktion von Distickstoff zu Ammoniak mit Molybdän: Theorie und Experiment (2008)

Abstract Molybdänkomplexe mit dem Triamidoamin‐Liganden [(RNCH 2 CH 2 ) 3 N] 3− (R=3,5‐(2,4,6‐iPr 3 C 6 H 2 ) 2 C 6 H 3 ) katalysieren die Reduktion von Distickstoff unter Einwirkung von Protonen und Elektronen zu Ammoniak. Die Protonen stammen dabei aus 2,6‐Dimethylpyridinium‐Ionen, die Elektronen aus Decamethylchromocen, die Reaktion verläuft bei 22 °C und 1 atm Druck. Mögliche Intermediate dieser Reduktion und ihre Reaktionscharakteristik wurden in mehreren theoretischen Arbeiten untersucht. So wurden DFT‐Rechnungen des Mo‐Komplexes [(HIPTNCH 2 CH 2 ) 3 N]Mo (HIPT=Hexaisopropylterphenyl=3,5‐(2,4,6‐iPr 3 C 6 H 2 ) 2 C 6 H 3 ) durchgeführt, der wie die katalytisch wirksamen Intermediate den Liganden Triamidoamin enthält. In diesem Kurzaufsatz vergleichen wir für jeden angenommenen Teilschritt der Katalysereaktion aktuelle theoretische Befunde und experimentelle Ergebnisse.
H5N1 influenza vaccine induces a less robust neutralizing antibody response than seasonal trivalent and H7N9 influenza vaccines (2017)

Conventional inactivated avian influenza vaccines have performed poorly in past vaccine trials, leading to the hypothesis that they are less immunogenic than seasonal influenza vaccines. We tested this hypothesis by comparing the immunogenicity of the H5N1 and H7N9 vaccines (avian influenza vaccines) to a seasonal trivalent inactivated influenza vaccine in naïve ferrets, administered with or without the adjuvants MF59 or AS03. Vaccine immunogenicity was assessed by measuring neutralizing antibody titers against hemagglutinin and neuraminidase and by hemagglutinin -specific IgG levels. Two doses of unadjuvanted vaccines induced low or no HA-specific IgG responses and hemagglutination-inhibiting titers. Adjuvanted vaccines induced comparable IgG-titers, but poorer neutralizing antibody titers for the H5 vaccine. All adjuvanted vaccines elicited detectable anti- neuraminidase -antibodies with the exception of the H5N1 vaccine, likely due to the low amounts of neuraminidase in the vaccine. Overall, the H5N1 vaccine had poorer capacity to induce neutralizing antibodies, but not HA-specific IgG, compared to H7N9 or trivalent inactivated influenza vaccine.
Extreme Nonlinear Optics: Coherent X rays from Lasers (2005)

Ultrashort laser pulses can generate even shorter bursts of coherent soft x rays. The technology now makes it possible to manipulate atoms on attosecond time scales to create designer wavefunctions.
All optical cross-connect system using erbium-doped fiber-based optical switching unit (2006)

A novel bidirectional 2×2 optical switch technique, based on the conventional erbium-doped fibers (EDFs) with an operation of pumped or unpumped power level, is proposed and demonstrated experimentally. By using the amplification and absorption function of EDFs and a simply architecture design, the input signals can be switched to a suitable position. The response time of wavelength switching will approach about 2 ms. Moreover, the behavior and system performance of the proposed configuration also have been studied.