gebhart/patrepeval huggingface dataset:
TMEM33: a new stress-inducible endoplasmic reticulum transmembrane protein and modulator of the unfolded protein response signaling (2015)

Endoplasmic reticulum (ER) stress leads to activation of the unfolded protein response (UPR) signaling cascade and induction of an apoptotic cell death, autophagy, oncogenesis, metastasis, and/or resistance to cancer therapies. Mechanisms underlying regulation of ER transmembrane proteins PERK, IRE1α, and ATF6α/β, and how the balance of these activities determines outcome of the activated UPR, remain largely unclear. Here, we report a novel molecule transmembrane protein 33 (TMEM33) and its actions in UPR signaling. Immunoblotting and northern blot hybridization assays were used to determine the effects of ER stress on TMEM33 expression levels in various cell lines. Transient transfections, immunofluorescence, subcellular fractionation, immunoprecipitation, and immunoblotting were used to study the subcellular localization of TMEM33, the binding partners of TMEM33, and the expression of downstream effectors of PERK and IRE1α. Our data demonstrate that TMEM33 is a unique ER stress-inducible and ER transmembrane molecule, and a new binding partner of PERK. Exogenous expression of TMEM33 led to increased expression of p-eIF2α and p-IRE1α and their known downstream effectors, ATF4-CHOP and XBP1-S, respectively, in breast cancer cells. TMEM33 overexpression also correlated with increased expression of apoptotic signals including cleaved caspase-7 and cleaved PARP, and an autophagosome protein LC3II, and reduced expression of the autophagy marker p62. TMEM33 is a novel regulator of the PERK-eIE2α-ATF4 and IRE1-XBP1 axes of the UPR signaling. Therefore, TMEM33 may function as a determinant of the ER stress-responsive events in cancer cells.
Metabolic activation of carboxylic acids (2008)

This review demonstrates that metabolites of carboxylic acid drugs must be considered chemically reactive, and that the current knowledge about metabolic activation of this compound class can be a good starting-point for further studies on the consequences of chemically reactive metabolites.
Data Analytics for Continuous Renal Replacement Therapy: Historical Limitations and Recent Technology Advances (2016)

The enormous amount of data in CRRT should be collected and analyzed to enable adequate clinical decisions. Current CRRT technology presents significant limitations with consequent lack of rigorous analysis of technical data and relevant feedback. From a quality assurance perspective, these limitations preclude any systematic assessment of prescription and delivery trends that may be adversely affecting clinical outcomes. A detailed assessment of current practice limitations is provided together with several possible ways to address such limitations by a new technical tool.
Purified Excreted-Secreted Antigens from Trypanosoma cruzi Trypomastigotes as Tools for Diagnosis of Chagas' Disease (2006)

There is currently no "gold standard" test for the diagnosis of late-stage Chagas' disease. As a result, protection of the blood supply in areas where Chagas' disease is endemic remains problematic. A panel of 709 serum samples from subjects with confirmed Chagas' disease (n = 195), healthy controls (n = 400), and patients with other parasitic diseases (n = 114) was used to assess enzyme-linked immunosorbent assays (ELISAs) based on a concentrated extract of excretory-secretory antigens from either Brazil or Tulahuen strain Trypanosoma cruzi trypomastigotes (total trypomastigote excretory-secretory antigens [TESAs]). The total TESA-based assays had excellent overall sensitivity (100%) and specificity (>94%), except for cross-reactivity with Leishmania-infected sera. In an attempt to increase the specificity of the assay, immunoaffinity chromatography was used to purify the TESA proteins (TESA(IA) proteins). By Western blotting, a series of polypeptide bands with molecular masses ranging from 60 to 220 kDa were recognized by pooled sera positive for Chagas' disease. An ELISA based on TESA(IA) proteins had a slightly lower sensitivity (98.6%) but an improved specificity (100%) compared to the sensitivity and specificity of the total TESA protein-based ELISAs. A 60-kDa polypeptide was identified as a major contributor to the cross-reactivity with Leishmania. These data suggest the need for field validation studies of TESA- and TESA(IA)-based assays in regions where Chagas' disease is endemic.
Empirical evaluation of augmented information presentation on small form factors - navigation assistant scenario (2011)

Mobile Augmented Reality (MAR) enabled devices will have the capability to present a large amount of information in real time, based on sensors that determine proximity, visual reference, maps, and detailed information on the environment. This poses a challenge of presenting the information such that there is no cognitive overload for the user and the augmented information that is presented is useful and meaningful to the user. This study examined the user tolerance and identified acceptable values for the performance characteristics of the augmented information presented on - density of information, accuracy of information, delay in information presentation, and error rate. Results indicate that the amount of information presented depends on the type of activity that the user is interested in. For example, in the case of density of information - participants were interested in seeing about 7 items identified at a time. With 11 items, most were overwhelmed, but 4 items were not enough. However, desired information density depends on the information shown, and the participants wanted to control the type of information shown. The findings of the study can be used as design guidelines for MAR information overlay on small screens.
Enantio‐ and Diastereoselective 1,2‐Additions to α‐Ketoesters with Diborylmethane and Substituted 1,1‐Diborylalkanes (2016)

The catalytic enantioselective synthesis of boronate-substituted tertiary alcohols through additions of diborylmethane and substituted 1,1-diborylalkanes to α-ketoesters is reported. The reactions are catalyzed by readily available chiral phosphine/copper(I) complexes and produce β-hydroxyboronates containing up to two contiguous stereogenic centers in up to 99:1 e.r. and greater than 20:1 d.r. The utility of the organoboron products is demonstrated through several chemoselective functionalizations. Evidence indicates the reactions occur via an enantioenriched α-boryl-copper-alkyl intermediate.
CD45 regulates TCR-induced signalling through tyrosine phosphorylation of phospholipase CγI (1992)

Conference Article| February 01 1992 CD45 regulates TCR-induced signalling through tyrosine phosphorylation of phospholipase CγI Steven B. Kanner; Steven B. Kanner 1Bristol-Myers Squibb Pharmaceutical Research Institute, 3005 First Avenue, Seattle, WA 98121, U.S.A. Search for other works by this author on: This Site PubMed Google Scholar Jeffrey A. Ledbetter Jeffrey A. Ledbetter 1Bristol-Myers Squibb Pharmaceutical Research Institute, 3005 First Avenue, Seattle, WA 98121, U.S.A. Search for other works by this author on: This Site PubMed Google Scholar Author and article information Publisher: Portland Press Ltd Received: September 09 1991 Online ISSN: 1470-8752 Print ISSN: 0300-5127 © 1992 Biochemical Society1992 Biochem Soc Trans (1992) 20 (1): 178–184. https://doi.org/10.1042/bst0200178 Article history Received: September 09 1991 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation Steven B. Kanner, Jeffrey A. Ledbetter; CD45 regulates TCR-induced signalling through tyrosine phosphorylation of phospholipase CγI. Biochem Soc Trans 1 February 1992; 20 (1): 178–184. doi: https://doi.org/10.1042/bst0200178 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: PtdIns, phosphatidyl inositol, PLC, phospholipase C, Ins(1,4,5)P3, inositol 1,4,5-triphosphate, DAG, diacylglycerol, PTK, protein tyrosine kinase, pTyr, phosphotyrosine, mAb, monoclonal antibody, IL-2, interleukin-2, [Ca2+]i, intracellular free calcium ion concentration This content is only available as a PDF. © 1992 Biochemical Society1992 Article PDF first page preview Close Modal You do not currently have access to this content.
Multi-channel time-frequency data fusion (2003)

This paper proposes an efficient mechanism for the fusion of two noisy speech signals obtained by an array of two microphones using single-tap time-frequency filters and by taking into account the correct time delay of arrival (TDOA) of the speech source. Speech signals obtained by the microphones are transformed into a set of two complex time-frequency (TF) images. By knowing the correct TDOA, and therefore the associated phase difference between the signals at each frequency, it is possible to non-linearly filter both the real and the imaginary parts of the TF images. This will consist of a TF reward-punish filter that adjusts the amplitude of the TF blocks based upon the variation of their phase-difference with the ideal phase-difference defined by the TDOA. Simulation results show that the proposed technique can achieve a Signal-to-Noise Ratio (SNR) improvement of 15 dB when there, is strong Gaussian noise present (-20 dB initial SNR). When the original SNR is OdB, the simulated improvement is approximately 8 dB. It is also shown that although the proposed technique is a more general case of the adaptive beamformer (where the adaptive beamformer has a specific reward-punish characteristic), other reward-punish characteristics that are proposed in this paper can often surpass the performance of the ideal adaptive beamformer.
Trimetrexate: a second generation folate antagonist in clinical trial. (1987)

Trimetrexate is a promising new lipophilic antifolate with antitumor and antimicrobial activity, which is currently undergoing clinical trials. It differs from methotrexate in its transport and intracellular retention, and may be useful against tumors resistant to methotrexate because of impaired transport or deficient polyglutamylation. In preclinical studies, it has demonstrated a broader spectrum of antitumor activity than methotrexate, and appears to have synergistic antitumor activity when combined with a number of other agents. In clinical trials, the primary toxicity has been hematologic, with rapid recovery and no evidence of cumulative dose effect. Nonhematologic toxicity has been sporadic. Preliminary results of phase II trials indicate activity against breast, non-small-cell lung, and head and neck tumors. In addition, trimetrexate with simultaneous leucovorin rescue appears to be a promising new treatment for pneumocystis pneumonia in AIDS patients, with minimal toxic side effects.
Supplementary Table 1 from Integrated Next-Generation Sequencing and Avatar Mouse Models for Personalized Cancer Treatment (2023)

&lt;p&gt;PDF file - 802KB, Somatic mutations and Copy number variations.&lt;/p&gt;
ChemInform Abstract: 2,6‐METHANO‐2,6‐DEHYDRONORBORNANE: AN EXCEPTIONALLY STRAINED (3.1.1)PROPELLANE (1982)

Abstract Ausgehend von der Carbonsäure (Ia) wird nach ihrer Umwandlung in das Amid (Ib) durch Reduktion und nachfolgende Oxidation das Aminoxid (II) erhalten.
Quasi‐Seeded Growth of Ligand‐Tailored PbSe Nanocrystals through Cation‐Exchange‐Mediated Nucleation (2008)

Seeds of change: An unusually fast cation-exchange reaction at the nanoscale is an efficient tool for precise control of the nucleation of nanocrystals. As a model system, 3.2–14-nm PbSe nanocrystals are produced by the nucleation reaction of Pb2+ with transient SnSe nuclei (see picture). The synthesis allows surface derivatization of the PbSe nanocrystals with various ligands.
Acelerar la transformación digital en tiempos difíciles (2021)

Carlos Valdez (Perú) - Presentador; &#13;\nDiego Carrillo (Perú) - Expositor; &#13;\nJuan Pacheco (Perú) - Expositor; &#13;\nMarushka Chocobar (Perú) - Expositora; &#13;\nHideyuki Iwata (Japón) - Expositor; &#13;\nHugo Chang (Perú) - Expositor; &#13;\nRaúl Hugo - (Perú) - Expositor; &#13;\nMesías Guevara (Perú); &#13;\nSergio Salas (Perú) - Moderador
Characterization and modeling of fast traps in thermal agglomerating germanium nanocrystal metal-oxide-semiconductor capacitor (2008)

In this paper, the germanium (Ge) nanocrystals (NCs) are synthesized by using the rapid-thermal annealing and are embedded into a three-layer (SiO2∕NCs–Ge∕SiO2) capacitor structure. The samples with∕without the postmetallization annealing (PMA) treatment are investigated to compare and study the PMA affections. The charge storage characteristics of our samples are investigated with the capacitance-voltage (C-V) hystereses. The frequency independence of hysteresis windows is found and attributed to NCs as slow traps with a large characteristic time constant. The frequency-dependent C-V and conductance-voltage (G-V) experiments are further introduced to study the interface traps and the fast traps induced by the NC formation. In order to extract the related trap characteristics from the measured C-V and G-V, we propose to utilize the equivalent circuit and single-level trap model based on Shakley-Read-Hall theory. Three associated parameters including the areal trap density, trap conductance, and semiconductor capacitances are used to confirm that the single-level trap model is truly appropriate for our samples. It is then found from the model that the areal trap density is high and approaches almost uniform distribution along the valence band and bandgap but significantly reduced and then becomes decreased from valence band to the midgap after PMA treatment. In addition, after PMA treatment, the characteristic time constant becomes smaller for one order of magnitude at the same gate bias. It is attributed to the reduction of trap density and also agrees that the interface traps are dominant and has a small characteristic time constant.
Data from BAG3 Regulates Motility and Adhesion of Epithelial Cancer Cells (2023)

&lt;div&gt;Abstract&lt;p&gt;BAG3 protein binds to and regulates Hsp70 chaperone activity. The BAG3 protein contains a WW domain and a proline-rich region with SH3-binding motifs, suggesting that it may interact with proteins relevant to signal transduction, recruiting Hsp70 to signaling complexes and altering cell responses. BAG3 overexpression has been observed in human cancers. We show here that homozygous BAG3-deficient mouse embryonic fibroblasts (MEF) exhibit delayed formation of filopodia and focal adhesion complexes when freshly plated. BAG3-deficient MEFs show reduced cell motility in culture. We observed that endogenous BAG3 protein is highly expressed in many human epithelial cancer cell lines, especially adenocarcinomas. Gene transfer–mediated overexpression of BAG3 increased motility of Cos7 cell and several human cancer cell lines, including breast cancer MCF7 and prostate cancer DU145 and ALVA31 cell lines. Conversely, reduction of BAG3 protein by RNA interference (RNAi) decreased cell motility in four of four epithelial tumor lines tested. We observed an influence of BAG3 on cell adhesion in culture. In Cos7 kidney epithelial cells, BAG3 protein partially colocalizes with actin at the leading edge of migrating cells, wherein active actin polymerization and nucleation occur. RNAi-mediated reductions in BAG3 expression were associated with decreased Rac1 activity, suggesting a role for BAG3 in regulating this small GTPase involved in actin-cytoskeleton dynamics. In mice, RNAi-mediated reductions in BAG3 in a human tumor xenograft suppressed invasion and metastasis &lt;i&gt;in vivo&lt;/i&gt;. Thus, the high levels of BAG3 protein seen in some epithelial cancer cell lines may be relevant to mechanisms of tumor invasion and metastasis. [Cancer Res 2007;67(21):10252–8]&lt;/p&gt;&lt;/div&gt;
Graphical user interface for the URUS project (2008)

This work was supported by projects: 'Ubiquitous networking robotics in urban settings' (E-00938), 'Robotica ubicua para entornos urbanos' (J-01225).
Determination of molecular symmetry axis(z) orientation via photoelectron angular distribution measurements (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDetermination of molecular symmetry axis(z) orientation via photoelectron angular distribution measurementsDavid J. Leahy, Katharine L. Reid, and Richard N. ZareCite this: J. Phys. Chem. 1991, 95, 21, 8154–8158Publication Date (Print):October 1, 1991Publication History Published online1 May 2002Published inissue 1 October 1991https://doi.org/10.1021/j100174a025Request reuse permissionsArticle Views111Altmetric-Citations16LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (648 KB) Get e-Alerts
Consensus report: indications for use of long-acting antimuscarinic tiotropium in the treatment of bronchial asthma (2015)

Relevant indications for tiotropium Respimat in adult patients with bronchial asthma (BA) were reviewed. This consensus represents a collaborative effort of members of the Executive Group of Russian Respiratory Society. The Group consisted of clinicians, pharmacologists and researchers with recognized expertise in therapeutic field. The draft of the recommendations was prepared by initiative group. Each point of the recommendation was discussed during Executive group meeting and was accepted by vote of majority members. Final version of the consensus statement was approved by each member of Executive group. As concluded, tiotropium Respimat could be recommended for adult patients with bronchial asthma as: 1) add-on therapy in patients with severe and moderate severe non-controlled BA on GINA step 3–5 (ICS and LABA, including oral steroids or other controllers, or high doses ICS monotherapy); 2) add-on therapy in patients with asthma-COPD overlap syndrome (ACOS); 3) add-on therapy to ICS in case of side effects, contraindications for use or individual intolerance of LABA; 4) additional indications to consider use of tiotropium Respimat may include: smoking, positive SABA reversibility test, predominant nocturnal asthma symptoms.
Sensory neuron-specific receptor activation elicits central and peripheral nociceptive effects in rats (2004)

The sensory neuron-specific G protein coupled receptors (SNSRs) have been described as a family of receptors whose expression in small diameter sensory neurons in the trigeminal and dorsal root ganglia suggests an implication in nociception. To date, the physiological function(s) of SNSRs remain unknown. Hence, the aim of the present study was to determine the effects of rat SNSR1 activation on nociception in rats. The pharmacological characterization of rat SNSR1 was initially performed in vitro to identify a specific ligand, which could be used subsequently in the rat for physiological testing. Among all ligands tested, gamma2-MSH was the most potent at activating rat SNSR1. Structure-activity relationship studies revealed that the active moiety recognized by rat SNSR1 was the C-terminal part of gamma2-MSH. The radiolabeled C-terminal part of gamma2-MSH, gamma2-MSH-6-12, bound with high affinity to membranes derived from rat skin and spinal cord, demonstrating the presence of receptor protein at both the proximal and distal terminals of dorsal root ganglia. To investigate the physiological role of SNSR, specific ligands to rat SNSR1 were tested in behavioral assays of pain sensitivity in rats. Selective rat SNSR1 agonists produced spontaneous pain behavior, enhanced heat and mechanical sensitivity when injected intradermally, and heat hypersensitivity when injected centrally, consistent with the localization of rat SNSR1 protein at central and peripheral sites. Together, these results clearly indicate that the SNSR1 plays a role in nociception and may provide novel therapeutic opportunities for analgesia.
Flow cytometric characterization of freshly isolated and culture expanded human synovial cell populations in patients with chronic arthritis (2010)

Flow cytometry can distinguish synovial cell populations in tissue digests. The preparation method can influence the detection levels of macrophage and B-cell populations. However, stromal cell markers seem not affected and quantification is possible, supporting flow cytometry tissue analysis as a tool to study these cell populations in arthritis.
A novel synthesis of pyrido[2,3‐b][1,5]benzodiazepines (1994)

Abstract A novel and convenient synthesis of the title compounds 4, 5, 11 , and 13 is described, involving the ring transformation of 1,5‐benzodiazepine derivatives 1a and 1b with active methylene compounds.
Visible quantum dots under reversed bias (1998)

Time-resolved photoluminescence (PL), steady-state PL, and electro-luminescence techniques have been used to characterize the carrier relaxation processes and carrier escape mechanisms in a self-assembled AlInAs quantum dots (SAQD) p-i-n laser structure under reversed bias. The measurements were performed between 5 K and room temperature on a ring mesa sample as a function of bias. At 100 K, the PL decay time originating from the SAQD decreases with increasing reversed bias from approximately 450 ps under flat band condition to approximately 150 ps for biases of -4 V. The data can be explained by a simple model based on electron recombination in the quantum dots or escape our of the dots. The escape can occur by one of three possible routes: direct tunneling out of the distribution of electron excited state level, thermally assisted tunneling of ground state electrons through the upper electron excited states or thermionic emission to the wetting layer.
Bounce-Overs: Fixed Object Impacts Followed by Rollovers (2004)

&lt;div class="htmlview paragraph"&gt;In this study, U.S. crash data was analyzed to better understand bounce-over rollovers. Crash data was reviewed to evaluate the distribution of bounce-over crashes and injuries, initiation objects and impact locations.&lt;/div&gt; &lt;div class="htmlview paragraph"&gt;In passenger cars, bounce-over crashes account for 8.4% of rollovers but involve 36.2% of the seriously injured belted drivers. Most bounce-overs are initiated by contact with narrow objects such as a pole, tree or barrier, or large objects such as a ditch or embankment. Contact often occurs in the front of the vehicle. After contact, the vehicle yaws and rolls, and serious injuries are often sustained to the head.&lt;/div&gt; &lt;div class="htmlview paragraph"&gt;Based on field data, a laboratory test was developed to simulate a narrow object bounce-over. The test consists of towing a vehicle laterally on a fixture towards a stationary, angled barrier resting in gravel. The moving fixture is decelerated and the vehicle is released. The vehicle front impacts the edge of the barrier, simulating a narrow object impact. The vehicle then yaws as its wheels dig into the gravel inducing a rollover.&lt;/div&gt;
Supp. Figure 7 from LIM Kinase Inhibitor Pyr1 Reduces the Growth and Metastatic Load of Breast Cancers (2023)

&lt;p&gt;In vivo analysis of the Pyr1 effect on LIMKs activity markers&lt;/p&gt;