gebhart/patrepeval huggingface dataset:
Abstract 2938: A novel OX40 agonist licenses systemic anti-tumor immunity to overcome local immune suppression induced by well-established tumors (2010)

Abstract Immune modulating reagents that trigger the T cell costimulatory molecules GITR and OX40 are being evaluated as anti-cancer agents in early-stage clinical trials. However, little pre-clinical information is available regarding the efficacy and mechanism(s) of action for these agents in the setting of advanced, well-established disease. To further characterize the molecular, cellular, and treatment-associated consequences of GITR and OX40 engagement, novel agonistic reagents directed against murine GITR and OX40 (ligand-Fc fusion proteins) were recently constructed and characterized in vitro. We now show that the growth of well-established, day 17 sarcomas is significantly inhibited or ablated by a short course of either treatment, with OX40L-Fc demonstrating superior anti-tumor efficacy over GITRL-Fc at comparable doses. Despite diminished frequencies of CD4+Foxp3+ regulatory T cells (Treg) in the tumor microenvironment (TME) 3 days post-treatment, the presence of myeloid-derived suppressor cells (MDSC) and inhibitory cytokines such as IL-10 and TGFβ appears unaltered and effector T cell production of the Type-1 cytokine IFNγ remains low. Treatment with either GITR or OX40 agonists, however, induces a coordinate increase in CCR7+ dendritic cells in the tumor-draining lymph nodes (TDLN), followed approximately 7 days after treatment by profound expansion of effector T cells expressing the Type-1 chemokine receptor CXCR3. By day 10 post-treatment, particularly with the OX40 agonist, T cells displaying a recently-activated effector phenotype accumulate in the tumor microenvironment and secrete high levels of IFNγ. Collectively, these data suggests a model in which the first level effect of costimulatory treatment is to direct CCR7+ dendritic cells from the TME to the draining lymph nodes. A secondary, systemic response reflects the expansion of highly-reactive CXCR3+ Type-1 T cells in the TDLN and their subsequent trafficking into the TME. The therapeutic efficacy demonstrated in this model by OX40L-Fc, and to a lesser extent GITRL-Fc, strongly supports the translation of such modalities into human clinical trials, while the mechanistic insight gained from these studies suggests that successful immunotherapies will license both local, and perhaps more importantly, systemic Type-1 T cell immunity. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 2938.
A mechanism for the photochemical induced combustion of H2/F2 mixtures inhibited by O2 (1980)

Using the most recent mechanisms and rate constants for the chemistry of the H2/F2/O2 molecular system, we have performed detailed modeling studies of the stability data of Levy and Copland and have shown that these data are explainable with reasonable accuracy. This same chemistry model has also been used to explain the variation in performance of a H2/F2 pulsed chemical laser with added O2. The results show that although there is some gain in laser performance by operating at low O2 concentrations, the inverse linear dependence on O2 concentration rules out any dramatic increase in performance.
Detecting Reaction Intermediates in Liquids on the Millisecond Time Scale Using Desorption Electrospray Ionization (2010)

Fliegende Zwischenstufen: Beim Beschuss eines oberflächengebundenen Komplexes mit geladenen Tröpfchen einer ligandhaltigen Lösung lassen sich Reaktionsintermediate detektieren (siehe Schaubild). Auf diese Weise wurden RuII-Katalysatoren mit Aminoalkohol-Liganden synthetisiert, die asymmetrische Transferhydrierungen von Ketonen und Iminen vermitteln. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
The CLAVATA receptor FASCIATED EAR2 responds to different CLE peptides by signaling through different downstream effectors (2017)

Abstract Meristems contain groups of indeterminate stem cells that are critical for organ initiation throughout plant development. The shoot apical meristem (SAM) maintains itself and initiates all shoot organs, such as leaves, floral organs and axillary branch meristems. Development and balanced proliferation of the SAM is regulated by a feedback loop between CLAVATA ( CLV ) and WUSCHEL ( WUS ) signaling. CLV signaling is initiated by secretion of the CLV3 peptide ligand, which is perceived directly or indirectly by a number of Leucine-Rich-Repeat (LRR) receptor-like kinases, including CLV1 and BARELY ANY MERISTEM (BAM) 1-3, and RECEPTOR-LIKE PROTEIN KINASE 2 (RPK2), as well as the receptor-like protein CLV2 in a complex with the CORYNE (CRN) pseudokinase. However, CLV2, and its maize ortholog FASCIATED EAR2 (FEA2) appear to function in signaling by several related CLV3/EMBRYO-SURROUNDING REGION (CLE) peptide ligands, including CLV3. Nevertheless, it remains unknown how CLV2 or FEA2 transmit specific signals from distinct CLE peptides. Here we show that FEA2 is involved in signaling from at least 2 distinct CLE peptides, ZmCLE7, a maize CLV3 ortholog, and ZmFON2-LIKE CLE PROTEIN1 (ZmFCP1), a newly identified CLE peptide functioning in SAM regulation. Signaling from these 2 different CLE peptides appears to be transmitted through 2 different candidate downstream effectors, COMPACT PLANT2 (CT2), the alpha subunit of the maize heterotrimeric G protein, and maize CRN. Our data provide a novel framework to understand how diverse signaling peptides can activate different downstream pathways through common receptor proteins.
Customizable cryptographic architecture for government and military communications applications (2005)

With the continued international proliferation of military communication technology, equipment vendors are challenged to provide communications security (COMSEC) solutions that are appropriate for these applications. Addressing these challenges is complicated by the fact that no encryption standard exists for international government and military communications applications. Harris Corporation has leveraged its expertise in encryption algorithm design, type 1 hardware design, and embedded system design to develop a cost-effective, customizable encryption algorithm for military and government communications applications. The algorithm is the second generation of the highly successful Citadel/sup /spl trade// cryptographic algorithm. It was designed against a military/government threat model and specifically addresses the rigorous requirements dictated by these applications. It does so in a manner that is both flexible and cost effective. This paper will begin with a brief review of the requirements for military-grade encryption products. It will continue with an overview of the algorithm design along with discussion on the features responsible for its military grade strength.
Methodical approach for tribo-electrical and topographical tailoring of Cu-Sn connectors with extended direct laser interference patterning (2022)

Due to increased electrification and the uprise of autonomous driving, the number of electrical contacts per passenger vehicle is continuously increasing. In the assembly line, these multipolar connectors are usually mated manually by the operator, without the possibility of using special equipment and, consequently, must therefore not exceed a standardized limit value for the mating force. By using extended direct laser interference patterning (xDLIP) technology, the insertion force of electrical contacts is effectively reduced, increasing the total number of available electrical poles per connector socket. In this study, we have evaluated a wide range of structural parameters (different feature sizes and orientations) on Sn-coated Cu connectors. For all configurations, we have measured the electrical contact resistance as well as the insertion force for one insertion cycle. Scanning electron microscopy, energy dispersive x-ray spectroscopy and focused ion beam cross-sections were used to assess the newly generated topographies and to reveal the microstructure of both, the coating and the substrate. Cu-Sn connectors tailored with xDLIP show a decreased insertion force while mating with only marginal changes in the electrical contact resistance (ECR) for the first insertion cycle compared to unprocessed standard connectors. Summarizing, this work presents a holistic, methodical approach to tailor connectors.
Multiple cellular proteins are recognized by the adeno‐associated virus Rep78 major regulatory protein and the amino‐half of Rep78 is required for many of these interactions (1997)

Adeno-associated virus (AAV) encoded Rep78 is a multi-functional protein which is required for AAV DNA replication, is able to regulate both AAV and heterologous gene expression at the transcriptional level, and appears necessary for site specific integration of AAV DNA into human chromosome 19. By comparison with the analogous replication protein of the polyomaviruses, large T antigen, it seemed likely that Rep78 would interact with cellular proteins to carry out at least some its functions. This study demonstrates that Rep78 is able to interact with multiple cellular proteins, from cellular extracts as measured by West(far)-western, coimmunoprecipitation, and Rep78-affinity chromatography analysis. Eight cellular proteins of approximately 180, 140, 120, 95, 75, 55, 45, and 35 kDa (+/- 10%), were observed to bind Rep78 in all three assay systems. Two others, of 30 and 24 kDa, were observed in two of three assay systems. Furthermore, using truncated Rep78 proteins, it is demonstrated that the amino-terminus is required for most Rep78-cellular protein interactions. However, the extreme carboxy-terminus of Rep78 was found to be all that is required for binding to the 55 kDa cellular protein.
Pharmacology of Venules (1981)

Department of Physiology, State University of New York, Downstate Medical Center, Brooklyn, New York
Optimized and enhanced DNA plasmid vector based in vivo construction of a neutralizing anti-HIV-1 envelope glycoprotein Fab (2013)

Monoclonal antibody preparations have demonstrated considerable clinical utility in the treatment of specific malignancies, as well as inflammatory and infectious diseases. Antibodies are conventionally delivered by passive administration, typically requiring costly large-scale laboratory development and production. Additional limitations include the necessity for repeat administrations, and the length of in vivo potency. Therefore, the development of methods to generate therapeutic antibodies and antibody like molecules in vivo, distinct from an active antigen-based immunization strategy, would have considerable clinical utility. In fact, adeno-associated viral (AAV) vector mediated delivery of immunoglobulin genes with subsequent generation of functional antibodies has recently been developed. As well, anon-viral vector mediated nucleic acid based delivery technology could permit the generation of therapeutic/prophylactic antibodies in vivo, obviating potential safety issues associated with viral vector based gene delivery. This delivery strategy has limitations as well, mainly due to very low in vivo production and expression of protein from the delivered gene. In the study reported here we have constructed an "enhanced and optimized" DNA plasmid technology to generate immunoglobulin heavy and light chains (i.e., Fab fragments) from an established neutralizing anti-HIV envelope glycoprotein monoclonal antibody (VRC01). This "enhanced" DNA (E-DNA) plasmid technology includes codon/RNA optimization, leader sequence utilization, as well as targeted potentiation of delivery and expression of the Fab immunoglobulin genes through use of "adaptive" in vivo electroporation. The results demonstrate that delivery by this method of a single administration of the optimized Fab expressing constructs resulted in generation of Fab molecules in mouse sera possessing high antigen specific binding and HIV neutralization activity for at least 7 d after injection, against diverse HIV isolates. Importantly, this delivery strategy resulted in a rapid increase (i.e., in as little as 48 h) in Fab levels when compared with protein-based immunization. The active generation of functional Fab molecules in vivo has important conceptual and practical advantages over conventional ex vivo generation, purification and passive delivery of biologically active antibodies. Further study of this technique for the rapid generation and delivery of immunoglobulin and immunoglobulin like molecules is highly relevant and timely.
Hybrid Solar/Natural Gas Power System (2013)

This paper describes the approach taken and progress toward developing a hybrid solar/natural gas power system based on the integration of parabolic dish concentrators and micro-channel and meso-channel chemical process receivers, with an advanced combinedcycle power system. The project includes developing a solar-methane reforming reactor with associated heat exchangers, a prototype of which has already been demonstrated and which has accomplished the conversion of concentrated solar energy into chemical energy at high efficiency. The prototype demonstrated the provision of a “solar thermochemical augment” of more than 20% to a chemical fuel (i.e., methane; which is available from natural gas, biogas or other sources). The system concept represents potentially “lowhanging fruit” for commercializing concentrating solar power technologies in that it makes use of solar concentrators and a powerblock – a combined-cycle power system – that already are in the process of being commercialized.
Abstract 804: Antitumor activity of the potent and novel FLT3 inhibitor HM43239 in acute myeloid leukemia (2018)

Abstract Introduction: Acute myeloid leukemia (AML) with FMS-like tyrosine kinase 3 (FLT3) mutations is associated with poor prognosis with a high risk of relapse after therapy and reduced overall survival. Activating mutations of internal tandem duplication (ITD) and tyrosine kinase domain point mutations (TKD) of FLT3 have been reported in approximately 30% of AML as oncogenic driver mutations. Currently, FLT3 inhibitors showed clinical benefits in the corresponding AML patients. In this study, we have characterized a novel FLT3 inhibitor, HM43239, and assessed the potential as a novel therapy for AML patients. Materials and Methods: HM43239 is a novel, highly potent FLT3 kinase inhibitor. This compound showed tight binding to FLT3 kinase in in silico docking model as a reversible Type I inhibitor. In vitro kinase assay was performed to identify kinase selectivity of HM43239. Standard proliferation assay, immunoblotting, and apoptosis analysis were carried out to validate the potency of HM43239 in AML cell lines. HM43239 was evaluated in MV-4-11 and MOLM-13 xenograft mice models. Tumor sizes were measured and tumor samples were analyzed of the mechanisms of action. Results: Among 191 kinases biochemically assayed, HM43239 showed the high selectivity toward FLT3 and AML associated other kinases (e.g. SYK, JAK and TAK1). IC50s' of HM43239 against FLT3 WT, FLT3 ITD and FLT3 D835Y kinases were 1.1 nM, 1.8 nM and 1.0 nM, respectively. HM43239 potently inhibited the growth of AML cell lines harboring FLT3 ITD mutation, such as MV4-11 (IC50: 1.3 nM), MOLM-13 (5.1 nM) and MOLM-14 (2.9 nM). Furthermore, HM43239 effectively inhibited the phosphorylation levels of FLT3 and of downstream kinases related with cell proliferation. In addition, caspase 3/7-dependent apoptosis was induced by HM43239 in AML cell lines expressing FLT3 ITD mutant. And HM43239 inhibited proliferation and induced apoptosis of leukemic stem cell (LSC) marker-expressing KG1a cells (CD34+/CD38- cells) suggesting that the possibility for targeting LSC. HM43239 showed the excellent dose proportional antitumor activity in mouse models xenografted with both MV4-11 and MOLM-13 cell line without any significant toxicity. Moreover, we identified in vivo modulation of related targets (p-FLT3 &amp; p-STAT5) in AML cell with FLT3 mutant. Conclusion: Taken together, HM43239 has demonstrated the potential therapeutic efficacy for the treatment of AML patients and implicated the mechanism of overcoming resistance and preventing relapse. Citation Format: Miyoung Lee, Young Eun Ha, Mi Jin Moon, Joo-Yun Byun, HyunKyung Yu, SeokJong Kang, JaeHo Lee, Kyuhang Lee, Eunkyung Kim, Eunyoung Kim, Ho Jeong Lee, YoungHoon Kim, YoungGil Ahn, KweeHyun Suh, Sun-Jin Kim. Antitumor activity of the potent and novel FLT3 inhibitor HM43239 in acute myeloid leukemia [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 804.
In situ environmentally-isolated wear tester (2023)

The present invention relates to an in situ testing system, which includes a wear tester and an enclosure. The wear tester is configured to apply a normal load, by way of a tip, to a surface of the test sample. Use of an enclosure allows such wear testing to be conducted in a controlled environment.
A hitchcock assisted video edited night at the opera (2002)

Hitchcock is a semi-automatic video editing system. This video shows users collaboratively authoring a home video.
Seasonal variations in mortality and clinical indicators in international hemodialysis populations from the MONDO registry (2015)

Significant global seasonal variations in mortality, pre-SBP, IDWG, albumin and CRP were observed. Seasonal variations in mortality were most pronounced in temperate climate zones.
Prognose der österreichischen Wirtschaft 2017-2018: Heimisches Konjunkturhoch gestützt von weltweitem Aufschwung (2017)

HeimischesKonjunkturhoch gesttzt von weltweitem Aufschwung Die Weltwirtschaft befindet sich in einem Aufschwung, der nahezu alle Regionen erfasst.Im Einklang damit drfte das heimische Wirtschaftswachstum in den Jahren 2017 und 2018 2.6 % bzw.2.1 % betragen.Investitionen und Exporte sollten krftig zulegen und die Konsumnachfrage robust bleiben.Die Inflation drfte 2 % betragen.Aufgrund des starken Beschftigungswachstums wird die Zahl der Arbeitslosen wohl zurckgehen, die Arbeitslosenquote aber auf einem hohen Niveau verharren.Die Hochkonjunkturphase sollte dringend fr Strukturreformen genutzt werden.1. Einleitung und Zusammenfassung Die sterreichische Wirtschaft verzeichnet gegenwrtig die strkste Wachstumsphase seit Mitte der 2000er Jahre.Nachdem die heimische Volkswirtschaft im Vorjahr auf einen soliden Wachstumskurs einschwenkt hatte, beschleunigte sich das Wachstum seit dem dritten Quartal des Vorjahres merklich.Gemessen an der Trend-Konjunktur-Komponente betrug das Wirtschaftswachstum in den ersten beiden Quartalen 2017 gut 0.8 % gegenber dem Vorquartal.Insbesondere die Investitionen und die Exporte legten krftig zu und die private Konsumnachfrage blieb robust.Die Frhindikatoren deuten auf eine Fortsetzung des Aufschwungs hin.Vor diesem Hintergrund hebt das Institut seine Wachstumsprognose neuerlich an.Fr das Jahr 2017 wird nunmehr ein Wachstum von 2.6 % erwartet.Damit liegt die heimische Wirtschaftsdynamik erstmals seit dem Jahr 2013 wieder merklich ber der im Euroraum (2.0 %).Im nchsten Jahr sollte die heimische Wirtschaftsleistung um 2.1 % zulegen.Seit Mitte des Vorjahres verzeichnet die Weltwirtschaft einen soliden Aufschwung.Nach einem schwcheren Start in das heurige Jahr hat die Weltwirtschaft im zweiten Quartal wieder merklich angezogen.Dabei ist die Konjunktur nahezu in allen groen Volkswirtschaften gut
On reconfiguration latency in fault-tolerant system (2002)

Digital computers embedded in critical applications such as flight controls should be equipped with appropriate fault-tolerance schemes to ensure their reliable and safe operation in the presence of component failures. System reconfiguration, which enhances reliability by dynamically using spatial redundancy, is generally the most time-consuming fault-/error- handling stage. The reconfiguration latency, defined as the time taken for reconfiguring a system upon failure detection or mode change, depends on many parameters, including the size of application programs and data, the CPU and memory speed, built-in testing capabilities, the type of spares to use, the system architecture, and the reconfiguration strategy used. In this paper, we classify the reconfiguration techniques into four types: reconfigurable duplication, reconfigurable N-modular redundancy (NMR), backup sparing, and graceful degradation. For each type of reconfiguration, we evaluate the reconfiguration latency by using several parameters accounting for the aforementioned parameters, and determine if this type of reconfiguration can meet the application required latency.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
KOREAN SOCIETY FOR BIOINFORMATICS (KSBI) (2006)

This article gives the brief description of the Korean Society for Bioinformatics.
Creating nano-sized contact openings in the thick dielectric-based passivation layers for ultrathin CIGS solar cells: A cost and time-effective approach (2021)

In this contribution, a cost- and time- effective contacting approach is presented for dielectric-based rear surface passivation layers up to 30 nm. Nano-size and homogenously distributed openings are realized in AlOx passivation layer by selenization of LiF salt on top, and monitored via SEM. Further, this layer was implemented into sub-micron thick CIGS solar cells. Consequently, contact openings facilitate the current collection through the dielectric layer up to 30 nm thick. However, solar cell parameters remain the same, even worse than the reference. Thus, this approach is still open for optimizations.
A cell atlas of the fly kidney (2021)

SUMMARY Like humans, insects rely on precise regulation of their internal environments to survive. The insect renal system consists of Malpighian tubules and nephrocytes that share similarities to the mammalian kidney. Studies of the Drosophila Malpighian tubules and nephrocytes have provided many insights into our understanding of the excretion of waste products, stem cell regeneration, protein reabsorption, and as human kidney disease models. Here, we analyzed single-nucleus RNA sequencing (snRNA-seq) data sets to characterize the cell types of the adult fly kidney. We identified 11 distinct clusters representing renal stem cells (RSCs), stellate cells (SCs), regionally specific principal cells (PCs), garland nephrocyte cells (GCs) and pericardial nephrocytes (PNs). Analyses of these clusters revealed many new interesting features. For example, we found a new, previously unrecognized cell cluster: lower segment PCs that express Esyt2 . In addition, we find that the SC marker genes RhoGEF64c , Frq2 , Prip and CG10939 regulate their unusual cell shape. Further, we identified transcription factors specific to each cluster and built a network of signaling pathways that are potentially involved in mediating cell-cell communication between Malpighian tubule cell types. Finally, cross-species analysis allowed us to match the fly kidney cell types to mouse kidney cell types and planarian protonephridia - knowledge that will help the generation of kidney disease models. To visualize this dataset, we provide a web-based resource for gene expression in single cells ( https://www.flyrnai.org/scRNA/kidney/ ). Altogether, our study provides a comprehensive resource for addressing gene function in the fly kidney and future disease studies.