gebhart/patrepeval huggingface dataset:
P92Regulatory and functional analyses of Neuraminidase-1 in inflammatory processes after myocardial ischemia/reperfusion (2014)

Purpose: Implementation of PTA as standard treatment for patients with myocardial infarction (MI) reduced the mortality in the early phase. However, morbidity due to adverse cardiac remodelling has increased. In MI-patients, serum concentrations of free sialic acids are significantly elevated. Those are released from glycoconjugates by neuraminidases (Neu). Neu play important roles in various biological processes, i.e. inflammation, differentiation and metabolism. Here, we investigate the role and regulation of Neu1 in monocytes/macrophages (Mo/Mϕ) after cardiac ischemia/reperfusion (I/R). Methods & results: Wild type mice undergoing cardiac I/R operation (50 min occlusion of the LAD followed by reperfusion) showed a significant up-regulation of Neu1 mRNA and protein expression in the ischemic area 3 d after I/R compared to sham operated mice (191±40%, p<0.001), whereas there was no difference between the two groups 14 d after I/R. Neu1 enzyme activity was also significantly increased on day 3 (403±90 mU/mg protein vs. 280±70 mU/mg protein, p<0.01). In the ischemic left ventricle, immunhistochemistry revealed a strong Neu1 expression in Mϕ and CD11b+ Mo/Mϕ isolated by MACS from infarcted murine hearts 3 d after I/R showed up-regulated Neu1 mRNA expression compared to CD11b+ Mo from the spleen (+79±55%). In vitro differentiation of the human monocytic cell line THP-1 into Mϕ by PMA increased the expression and activity of Neu1 (333±61 vs. mock, p<0.01). SiRNA-mediated down-regulation of Neu1 in THP-1 Mϕ resulted in a significant reduction of the expression of pro-inflammatory cytokines (IL-6: 47±11%, TNF-α: 68±7%, IL-1β: 78±8% vs. ctrl-siRNA, p<0.05), but had no effect on expression of the anti-inflammatory cytokine IL-10. The stimulation of THP-1 Mϕ with IL-6 had no effect on Neu1 expression. Furthermore, differentiation of primary human CD14+ Mo into pro-inflammatory M1 Mϕ by GM-CSF was associated with an up-regulation of Neu1 mRNA on day 4 (438±360%), whereas anti-inflammatory M2 (M-CSF) displayed no changes in Neu1 expression compared to CD14+ Mo. A possible regulatory mechanism of Neu1 expression in Mo/Mϕ could be the microRNA-125, as overexpression of miR-125a in primary human M1 resulted in down-regulation of Neu1 protein expression. Conclusion: Neu1 expression increases in the early phase after I/R and is mainly found in invading Mo/Mϕ, so that it seems to participate in the pro-inflammatory reactions via regulating cytokine expression. Therefore Neu1 may display a new therapeutic target after reperfusion of the infarcted heart to attenuate adverse effects of inflammation.
PERFORMANCE EVALUATION OF THE UCL (2010)

An ongoing project is being carried out to develop a High Purity Germanium (HPGe) Compton camera for medical applications. The Compton camera offers many potential advantages over other imaging modalities used in nuclear medicine. These advantages include a wide field of view, the ability to reconstruct 3D images without tomography, and the fact that the camera can have a portable lightweight design due to absence of heavy collimation. In this paper, the imaging performance of the University College London (UCL) HPGe Compton camera has been experimentally evaluated and reported. The imaging performance with different gamma-ray source energies was evaluated. The measured angular resolutions are 4.2°, 3.5°, and 2.8 ° ± 0.4 for 356, 511 and 662 keV gamma- ray energies respectively. Images have been taken of a variety of test objects, including line and circular source distributions. This paper summaries the results to date and present the current state of the camera performance.
Manual and Automated Direct Sequencing of Product Generated by the Polymerase Chain Reaction (2003)

Identification of point mutations has been facilitated by a number of techniques, including transfection assays, oligonucleotide hybridization, electrophoretic migration of heteroduplexes, RNase mismatch analysis, direct sequencing, and DNA-polymerase catalyzed amplification. The large number of available techniques emphasizes the importance of developing rapid and reliable methods to identify molecular changes in genes. To date, we have concentrated on exploiting DNA-polymerase catalyzed amplification methods (1,2) in conjunction with direct manual and automated DNA sequencing to detect point mutations in the dihydrofolate reductase (DHFR) gene of methotrexate-resistant cells.
Consumer Time Inconsistency: Evidence from a market experiment in the credit card market (2004)

This paper analyzes a unique dataset, which contains results of a large-scale experiment in the credit card market. Two strange phenomena that suggest time inconsistency in consumer behavior are observed: First, consumers prefer an introductory offer which has a lower interest rate with a shorter duration to that of a higher interest rate with a longer duration, even though they would benefit more should they choose the latter. Second, consumers are very reluctant to switch, and even those consumers, who have switched before, fail to switch again later. A multi-period model with complete information is studied to show that the standard exponential preferences can't explain the observed behavior but the hyperbolic preferences can. Furthermore, we study a dynamic model where realistic random shocks are incorporated. Estimation results show that consumers have severe self-control problem, with a present-bias factor beta=0.8, and that the average switching cost is $150. With the estimated parameters, the dynamic model can replicate quantitative features of the data.
Antibody–Drug Conjugates Derived from Cytotoxic seco-CBI-Dimer Payloads Are Highly Efficacious in Xenograft Models and Form Protein Adducts In Vivo (2019)

This work discloses the first examples of antibody-drug conjugates (ADCs) that are constructed from linker-drugs bearing dimeric seco-CBI payloads (duocarmycin analogs). Several homogeneous, CD22-targeting THIOMAB antibody-drug conjugates (TDCs) containing the dimeric seco-CBI entities are shown to be highly efficacious in the WSU-DLCL2 and BJAB mouse xenograft models. Surprisingly, the seco-CBI-containing conjugates are also observed to undergo significant biotransformation in vivo in mice, rats, and monkeys and thereby form 1:1 adducts with the Alpha-1-Microglobulin (A1M) plasma protein from these species. Variation of both the payload mAb attachment site and length of the linker-drug is shown to alter the rates of adduct formation. Subsequent experiments demonstrated that adduct formation attenuates the in vitro antiproliferation activity of the affected seco-CBI-dimer TDCs, but does not significantly impact the in vivo efficacy of the conjugates. In vitro assays employing phosphatase-treated whole blood suggest that A1M adduct formation is likely to occur if the seco-CBI-dimer TDCs are administered to humans. Importantly, protein adduct formation leads to the underestimation of total antibody (Tab) concentrations using an ELISA assay but does not affect Tab values determined via an orthogonal LC-MS/MS method. Several recommendations regarding bioanalysis of future in vivo studies involving related seco-CBI-containing ADCs are provided based on these collective findings.
Native hole trap in bulk GaAs and its association with the double-charge state of the arsenic antisite defect (1985)

We have identified a dominant hole trap in p-type bulk GaAs employing deep level transient and photocapacitance spectroscopies. The trap is present at a concentration up to about 4×1016 cm−3, and it has two charge states with energies 0.54±0.02 and 0.77±0.02 eV above the top of the valence band (at 77 K). From the upper level the trap can be photoexcited to a persistent metastable state just as the dominant midgap level, EL2. Impurity analysis and the photoionization characteristics rule out association of the trap with impurities Fe, Cu, or Mn. Taking into consideration theoretical results, it appears most likely that the two charge states of the trap are the single and double donor levels of the arsenic antisite AsGa defect.
More than you think? The Floating Mass Transducer (FMT) as a microphone – temporal bone measurements (2019)

Research using an earcanal-eardrum-model showed that the FMT possibly works as a microphone, so to say "reverse". Temporal bone measurements were performed to determine the sensitivity and frequency range. The FMT working as a middle ear microphone might lead to a fully implantable CI.
CHOROIDAL THICKNESS CHANGES AFTER DIABETES TYPE 2 AND BLOOD PRESSURE CONTROL IN A HOSPITALIZED SITUATION (2014)

Diabetic patients showed a significant increase of choroidal thickness after the intensive control. Various ophthalmologic and systemic parameters seem to affect the choroidal thickness changes. This may be related to the progression of retinopathy after acute glycemic control.
A package demonstration with solder free compliant flexible interconnects (2010)

Flexible, stress-engineered spring interconnects are a novel technology potentially enabling room temperature assembly approaches to building highly integrated and multi-chip modules (MCMs). Such interconnects are an essential solder-free technology facilitating the MCM package diagnostics and rework. Previously, we demonstrated the performance, functionality, and reliability of compliant micro-spring interconnects under temperature cycling, humidity bias and high-current soak. Currently, we demonstrate for the first time the package with the 1st level conventional fine pitch C4 solder bump interconnects replaced by the arrays of microsprings. Dedicated CMOS integrated circuits (ICs) have been assembled onto substrates using these integrated microsprings. Metrology modules on the ICs are designed and used to characterize the connectivity and resistance of each microspring site.
Baveno VII Criteria Is an Accurate Risk Stratification Tool to Predict High-Risk Varices Requiring Intervention and Hepatic Events in Patients with Advanced Hepatocellular Carcinoma (2023)

The Baveno VII criteria are used in patients with liver cirrhosis to predict high-risk varices in patients with liver cirrhosis. Yet its use in patients with advanced hepatocellular carcinoma (HCC) has not been validated. HCC alone is accompanied with a higher variceal bleeding risk due to its association with liver cirrhosis and portal vein thrombosis. The use of systemic therapy in advanced HCC has been thought to further augment this risk. Upper endoscopy is commonly used to evaluate for the presence of varices before initiation of treatment with systemic therapy. Yet it is associated with procedural risks, waiting time and limited availability in some localities which may delay the commencement of systemic therapy. Our study successfully validated the Baveno VI criteria with a 3.5% varices needing treatment (VNT) missed rate, also with acceptable <5% VNT missed rates when considering alternative liver stiffness (LSM) and platelet cut-offs. The Baveno VII clinically significant portal hypertension rule-out criteria (LSM < 15 kPa and platelet >150 × 10<sup>9</sup>/L) also revealed a low frequency (2%) of hepatic events, whilst the rule-in criteria (LSM > 25 kPa) was predictive of a higher proportion of hepatic events (14%). Therefore, our study has successfully validated the Baveno VII criteria as a non-invasive stratification of the risk of variceal bleeding and hepatic decompensation in the HCC population.
Supplementary Figure 1 from Anaplastic, Plasmablastic, and Plasmacytic Plasmacytomas of Mice: Relationships to Human Plasma Cell Neoplasms and Late-Stage Differentiation of Normal B Cells (2023)

Supplementary Figure 1 from Anaplastic, Plasmablastic, and Plasmacytic Plasmacytomas of Mice: Relationships to Human Plasma Cell Neoplasms and Late-Stage Differentiation of Normal B Cells
Clinical Comparison of Conventional and Mobile Endockscope Videocystoscopy Using an Air or Fluid Medium (2017)

The ES mobile cystoscopy system may be a reasonable option in settings where electricity, sterile fluid irrigant, or access to CVC equipment is unavailable.
Photoacclimation of Chlorella vulgaris to Red Light from Light‐Emitting Diodes Leads to Autospore Release Following Each Cellular Division (1996)

Abstract The detailed light requirement for photosynthesis and photoautotrophic cell growth can be assessed using solid state technology. Advanced light‐emitting diodes (LEDs), constructed with double‐power double‐heterostructure (DDH) gallium aluminum arsenide (GaAlAs) chips, were examined for their ability to support mass culture of the eucaryotic alga Chlorella vulgaris . LEDs with peak emittance of 680 nm (with half‐power band width of 20 nm) were used as a sole light source for the cultivation of C. vulgaris . Fluorescent light (FL) served as a control. The final cell mass and specific cellular growth rate under LEDs were comparable to those obtained under full‐spectrum light (FL). The narrow‐spectrum monochromatic red light was found to reduce the average cell volume from 60 μm 3 to 30 μm 3 , and to make the size distribution and the per cell DNA distribution narrower, but did not affect the total biomass production. By switching light sources, the two distinct cell population states (obtained under red LEDs and FL, respectively) were found to be interchangeable. Two parametric flow cytometric analyses showed that the cells grown under red LED light had a more uniform DNA content at all cell sizes, as compared to cells grown under FL. These results show that the critical cell size for releasing autospores under red LED is smaller than that under FL. The number of autospores in one mother cell when grown under LED light appeared to be two, so that the mother cells break up after only one round of DNA replication. Although the solid state LED light source altered the cell cycle behavior of C. vulgaris , it can be used as an effective light source for autotrophic growth. Use of LEDs therefore promises to advance the current state of algal photobioreactors due to their efficiency, smallness, reliability, long lifetime, and desirable light characteristics.
Utilizing Artificial Intelligence to Determine Bone Mineral Density Via Chest Computed Tomography (2020)

Our results show that this AI prototype can successfully determine BMD in moderate correlation with DEXA. Combined with other AI algorithms directed at evaluating cardiac and lung diseases, this prototype may contribute to future comprehensive preventative care based on a single chest CT.
Rationale for testing chemokine inhibitors (Virustats) for prevention of HIV-1 transmission. (2001)

HIV infection is dependent upon utilization of chemokine co-receptors to facilitate cellular entry. Of the numerous chemokine co-receptors that can be used by HIV for in vitro infection, the beta chemokine receptor CCR5 plays the most important role in acquisition of HIV-1 infection in vivo. High levels of beta chemokines, MIP-1a, MIP-1b and RANTES can block HIV-1 replication in vitro and persons who are homozygous for 32 base pair deletion in the coding sequences for CCR5 that results in failure of surface expression of this receptor are protected from acquisition of HIV-1 infection. This is reflected in a resistance to infection by CCR-5 utilizing HIV-1 strains in vitro. Targeting this coreceptor in vivo may be better tolerated than other host-directed therapies since there is no deleterious phenotype associated with the d32 homozygous state. The IP/CP chemokine receptor inhibitor team is examining a number of N-terminus modified beta chemokines for their utility as inhibitors of HIV-1 replication and as agents that may be used to block mucosal HIV-1 transmission. The lead molecule-aminooxypentane (AOP)-RANTES is more active than RANTES in blocking HIV-1 propagation and is active in nanomolar or subnanomolar concentrations against numerous HIV-1 isolates including selected isolates from clades A,B,C,D,E and F. It is important to note that AOP-administration does not induce monocyte chemotaxis thus suggesting that mucosal administration of this compound may not induce inflammatory responses. Although AOP-RANTES and other chemokine inhibitors can compete with natural ligands for chemokine receptor binding, inhibition of fusion activity may be more dependent upon induction of coreceptor internalization that persists for many hours after drug is withdrawn from culture. Thus topical administration of these compounds may provide durable 'resistance' to HIV-1 cellular entry. In the hu-PBL-SCID mouse model, AOP-RANTES can decrease the magnitude of HIV-1 replication but did not prevent infection and in 6 of ten mice challenged, NNY-RANTES blocked parenteral acquisition of HIV-1 infection but in two of the infected mice, emergence of X4 utilizing strains was demonstrated. A number of additional modified chemokine inhibitors are under study that inhibit HIV-1 replication in subnanomolar concentrations and provide durable suppression of CCR5 surface expression. The effects of these agents on vaginal inflammatory responses, on vaginal flora, pH and other STD pathogens remains to be determined. Likewise, the immunogenicity of these large molecules must be examined. The development of these molecules as 'virustats'-inhibitors of mucosal HIV transmission may provide an inexpensive method for women to protect against sexual transmission of HIV.
Non-stick syringe needles: Beneficial effects of thin film metallic glass coating (2016)

This paper reports on the use of Zr-based (Zr53Cu33Al9Ta5) thin film metallic glass (TFMG) for the coating of syringe needles and compares the results with those obtained using titanium nitride and pure titanium coatings. TFMG coatings were shown to reduce insertion forces by ∼66% and retraction forces by ∼72%, when tested using polyurethane rubber block. The benefits of TFMG-coated needles were also observed when tested using muscle tissue from pigs. In nano-scratch tests, the TFMG coatings achieved a coefficient of friction (COF) of just ∼0.05, which is about one order of magnitude lower than those of other coatings. Finite-element modeling also indicates a significant reduction in injection and retraction forces. The COF can be attributed to the absence of grain boundaries in the TFMG coating as well as a smooth surface morphology and low surface free energy.
Identification and function of enhancers in the human genome (2016)

The study of gene regulation has rapidly advanced by leveraging next-generation sequencing to identify and characterize the cis and trans elements that are critical for defining cell identity. These advances have paralleled a movement towards whole genome sequencing in clinics. These two tracks have increasingly synergized to underscore the importance of cis-regulatory elements in development as well produce countless studies implicating these elements in human disease. Other studies have emphasized the clinical phenotypes associated with variation or mutations in trans factors, including non-coding RNAs and chromatin regulators. These studies highlight the importance of obtaining a comprehensive understanding of mammalian gene regulation for predicting the impact of genetic variation on patient phenotypes. Currently lagging behind the generation of vast datasets and annotations is our ability to examine these putative elements in the dynamic context of a developing organism.
Grating Writing and Growth at 325nm in Non-Hydrogenated Silica Fiber (2010)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text G. E. Town, W. Yuan, A. Stefani, and O. Bang, "Grating Writing and Growth at 325nm in Non-Hydrogenated Silica Fiber," in Advanced Photonics & Renewable Energy, OSA Technical Digest (CD) (Optica Publishing Group, 2010), paper JThA22. Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
Anaerobic bacterial infections causing osteomyelitis/arthritis in a dog. (1992)

A 3-year-old German Shepherd Dog was examined for lameness, signs of pain, swelling, a draining fistulous tract, and osteolysis after a dog bite on the left carpus. After failure of the lesion to respond to several antibiotics, Peptostreptococcus sp and Propionibacterium sp were isolated from swab specimens and then from surgically collected bone and soft tissue specimens. The bone fragments had mild purulent osteomyelitis associated with numerous gram-positive rods and cocci. The dog was successfully treated by surgical debridement of the lesion and clindamycin administration.
Accurate conduction and switching loss models of IGBTs for resonant converter design (2004)

This paper presents an accurate switching and conduction loss model for zero current switching (ZCS) and zero voltage switching (ZVS) IGBTs. Mathematical modelling is carried out giving careful consideration to all the significant features and dependant parameters, namely collector current, resonant frequency and case temperature. Model parameter extraction is carried out using the experimental results from two test circuits. The model shows excellent agreement with the experimental results and SPICE simulation results obtained using a physical IGBT model.
Anahtarlamalı bir relüktans motorun konum algılayıcısız hibrid gözlemci modeli (2004)

Anahtarlamali Reluktans Motorlar (ARM) hiz ve konum denetimi icin hassas rotor konum bilgisine gereksinim duyarlar. Ancak, ARM’lerde konum algilayici kullanimi hem motor surme devresinin boyutlarini ve maliyetini arttirmakta hem de sistemin zor calisma ortamlarinda kullanimini sinirlandirmaktadir. Bu makalede, ARM’lerin konum algilayicisiz calistirilmasi icin yeni bir hibrid gozlemci yaklasimi sunulmaktadir. ARM modeli elektromekanik ve elektromanyetik olmak uzere iki modele ayristirilmistir. Ayristilan motor modeline uygulanan hibrid gozlemci modeli Luenberger ve kayma kipli gozlemci kuramlarindan elde edilmistir. Gelistirilen gozlemci ile kayma kipli gozlemcide titresim sebebiyle (chattering) meydana gelen sorunlar ve Luenberger gozlemcinin parametre hassasiyeti sorunu ortadan kaldirilmistir.
Contiguous conventional and plexiform schwannomas (2006)

The authors present clinical, imaging, and histological features of two adult patients found to harbor a conventional schwannoma contiguous with a deep plexiform schwannoma. One patient had neurofibromatosis (NF) Type 2 (NF2), and both intracranial (bilateral oculomotor, trigeminal, acoustic, and hypoglossal schwannomas as well as meningiomas) and intraspinal (schwannomas and meningiomas) lesions. The proximal forearm lesions consisted of a conventional schwannoma and an underlying plexiform component. The second patient, who did not have NF2, presented with a similar enlarging mass in the distal arm; two contiguous lesions were resected. Both patients exhibited distinct, readily identifiable, magnetic resonance imaging features. Plexiform schwannomas are rare lesions that occur sporadically or, on occasion, in association with NF2 or meningiomas with or without multiple schwannomas. The authors believe that a more careful examination of patients with NF2 may show that these people have a higher incidence of plexiform schwannoma than previously thought. Pathological confirmation is crucial in distinguishing plexiform schwannomas from plexiform neurofibromas, because the latter are directly associated with NF1 and have a significant tendency to undergo malignant transformation. To the best of the authors' knowledge, this is the first report of a conventional schwannoma contiguous with a deep plexiform schwannoma.