gebhart/patrepeval huggingface dataset:
Distinct Role for CD8 T Cells toward Cutaneous Tumors and Visceral Metastases (2008)

The growth of immunogenic tumors in immunocompetent individuals is one of the oldest conundrums in tumor immunology. Although the ability of mouse CD8+ T cells to control transplanted tumors is well documented, little is known about their impact on autochthonous tumors. To gain insight into the role of CD8+ T cells during the course of cancer development, we produced a novel model of spontaneous melanoma. The metallothionein (MT)-ret/AAD mouse is transgenic for the RET oncogene and the chimeric MHC molecule AAD (alpha1-alpha2 domains of HLA-A2 linked to alpha3 domain of H2-Dd). This model recapitulates the natural history of human melanoma, and expression of the AAD molecule makes it suitable for analyzing CD8+ T cell responses directed against peptide Ags that have been previously identified in HLA-A2+ melanoma patients. We found that, as tumors grow, mice develop a broad melanoma-specific CD8+ T cell response. Occurrence of cutaneous nodules is not affected by CD8+ T cell depletion, showing that although CD8+ T cells are functional, they have no effect on established cutaneous tumors. However, depleted mice die from visceral disease much earlier than controls, showing that CD8+ T cells control metastasis spreading and disease progression. Antigenic modulation is observed in visceral metastases, suggesting that visceral nodules may be subject to immunoediting. Our data demonstrate that growth of melanoma in the MT-ret/AAD model involves several tolerance mechanisms sequentially. They also reveal a different role for CD8+ T cells toward early stage of cutaneous tumors and late visceral metastatic stage of the disease.
High-density latch arrays (2023)

A system and device are provided for implementing memory arrays using high-density latch cells. The device includes an array of cells arranged into columns and rows. Each cell comprises a latch cell that includes a transmission gate, a pair of inverters, and an output buffer. Each row of latch cells is connected to at least one common node for addressing the row of latch cells, and each column of latch cells is connected to a particular bit of an input signal and a particular bit of an output signal. A register file may be implemented using one or more arrays of the high-density latch cells to replace any or all of the banks of SRAM cells typically used to implement the register file.
The Aachen MiniHLM—A Miniaturized Heart‐Lung Machine for Neonates With an Integrated Rotary Blood Pump (2010)

The operation of congenital heart defects in neonates often requires the use of heart-lung machines (HLMs) to provide perfusion and oxygenation. This is prevalently followed by serious complications inter alia caused by hemodilution and extrinsic blood contact surfaces. Thus, one goal of developing a HLM for neonates is the reduction of priming volume and contact surface. The currently available systems offer reasonable priming volumes for oxygenators, reservoirs, etc. However, the necessary tubing system contains the highest volumes within the whole system. This is due to the use of roller pumps; hence, the resulting placement of the complete HLM is between 1 and 2 m away from the operating table due to connective tubing between the components. Therefore, we pursued a novel approach for a miniaturized HLM (MiniHLM) by integrating all major system components in one single device. In particular, the MiniHLM is a HLM with the rotary blood pump centrically integrated into the oxygenator and a heat exchanger integrated into the cardiotomy reservoir which is directly connected to the pump inlet. Thus, tubing is only necessary between the patient and MiniHLM. A total priming volume of 102 mL (including arterial filter and a/v line) could be achieved. To validate the overall concept and the specific design we conducted several in vitro and in vivo test series. All tests confirm the novel concept of the MiniHLM. Its low priming volume and blood contact surface may significantly reduce known complications related to cardiopulmonary bypass in neonates (e.g., inflammatory reaction and capillary leak syndrome).
Quantum communication with semiconductor quantum dots (Conference Presentation) (2022)

Semiconductor quantum dots (QDs) are able to confine single charges on the nanoscale in all three dimensions of space, making them excellent systems for exploring quantum phenomena. In particular, QDs have demonstrated outstanding performance as sources of entangled and indistinguishable photon pairs, properties highly desired in the fields of quantum communication and -information processing. Here I report on the advances of QDs as potential resources for photonic quantum networks, which allow to overcome the fundamental range limitations of single photon-based applications. After an introduction to the underlying mechanisms of entangled photon pair generation, I demonstrate several building blocks of quantum networks, with quantum key distribution as a prime application.
1,1'-Carbonylbis(3-methylimidazolium) triflate: an efficient reagent for aminoacylations (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT1,1'-Carbonylbis(3-methylimidazolium) triflate: an efficient reagent for aminoacylationsAshis K. Saha, Henry Rapoport, and Peter SchultzCite this: J. Am. Chem. Soc. 1989, 111, 13, 4856–4859Publication Date (Print):June 1, 1989Publication History Published online1 May 2002Published inissue 1 June 1989https://pubs.acs.org/doi/10.1021/ja00195a043https://doi.org/10.1021/ja00195a043research-articleACS PublicationsRequest reuse permissionsArticle Views1669Altmetric-Citations77LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Effect of Sucrose Octanoate on Survival of Nymphal and Adult Diaphorina citri (Homoptera: Psyllidae) (2004)

Asian citrus psylla, Diaphorina citri Kuwayama (Homoptera: Psyllidae) was detected for the first time in the United States near Delray Beach, FL, on 2 June 1998 and is continuing to spread and multiply throughout southern Florida. This psyllid is the vector of Liberobacter asiaticum, a phloem-limited bacterium that causes citrus greening disease. This pathogen has not been found in the Western Hemisphere to date. Furthermore, high infestation levels of D. citri can impact citrus plant health, fruit quality, or yield. Replicated laboratory and spray booth bioassays were conducted to determine the insecticidal activity of a synthetic analog of natural sugar esters found in leaf trichomes of wild tobacco, Nicotiana gossei Domin, to nymphal and adult D. citri. Field trials were initiated in Fort Pierce, FL, in 2000 to determine activity of the sugar ester formulation (sucrose octanoate) on D. citri and other citrus pests, including immature Asian citrus leafminer, Phyllocnistis citrella Stainton and mites. Sucrose octanoate rates tested ranged from 400 to 8,000 ppm (0.1–2% formulated product). Our data suggest that both nymphal and adult D. citri as well as the mite complex tested would be equally controlled to levels of >90% at the higher concentrations of sucrose octanoate and that good coverage is key to efficacy.
Profiling Dynamic Patterns of Single-cell Motility (2022)

ABSTRACT Cell motility plays an essential role in many biological processes as cells move and interact within their local microenvironments. Current methods for quantifying cell motility typically involve tracking individual cells over time, but the results are often presented as averaged values across cell populations. While informative, these ensemble approaches have limitations in assessing cellular heterogeneity and identifying generalizable patterns of single-cell behaviors, at baseline and in response to perturbations. In this study, we introduce CaMI, a computational framework designed to leverage the single-cell nature of motility data. CaMI identifies and classifies distinct spatio-temporal behaviors of individual cells, enabling robust classification of single-cell motility patterns in a large dataset (n=74,253 cells). This framework allows quantification of spatial and temporal heterogeneities, determination of single-cell motility behaviors across various biological conditions, and provides a visualization scheme for direct interpretation of dynamic cell behaviors. Importantly, CaMI reveals insights that conventional cell motility analyses may overlook, showcasing its utility in uncovering robust biological insights. Together, we present a multivariate framework to classify emergent patterns of single-cell motility, emphasizing the critical role of cellular heterogeneity in shaping cell behaviors across populations. Teaser A computational framework to identify and classify single-cell motility patterns and phenotypic heterogeneity across biological conditions.
Comparison of Flutamide and Spironolactone in the Treatment of Hirsutism (1994)

Cusan, Leonello; Dupont, André; Gomez, José-Luis; Tremblay, Roland R.; Labrie, Fernand Author Information
Data from Clinical Benefit of Lapatinib-Based Therapy in Patients with Human Epidermal Growth Factor Receptor 2–Positive Breast Tumors Coexpressing the Truncated p95HER2 Receptor (2023)

&lt;div&gt;Abstract&lt;p&gt;&lt;b&gt;Purpose:&lt;/b&gt; A subgroup of human epidermal growth factor receptor 2 (HER2)–overexpressing breast tumors coexpresses p95HER2, a truncated HER2 receptor that retains a highly functional HER2 kinase domain but lacks the extracellular domain and results in intrinsic trastuzumab resistance. We hypothesized that lapatinib, a HER2 tyrosine kinase inhibitor, would be active in these tumors. We have studied the correlation between p95HER2 expression and response to lapatinib, both in preclinical models and in the clinical setting.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Experimental Design:&lt;/b&gt; Two different p95HER2 animal models were used for preclinical studies. Expression of p95HER2 was analyzed in HER2-overexpressing breast primary tumors from a first-line lapatinib monotherapy study (EGF20009) and a second-line lapatinib in combination with capecitabine study (EGF100151). p95HER2 expression was correlated with overall response rate (complete + partial response), clinical benefit rate (complete response + partial response + stable disease ≥24 wk), and progression-free survival using logistic regression and Cox proportional hazard models.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Results:&lt;/b&gt; Lapatinib inhibited tumor growth and the HER2 downstream signaling of p95HER2-expressing tumors. A total of 68 and 156 tumors from studies EGF20009 and EGF100151 were evaluable, respectively, for p95HER2 detection. The percentage of p95HER2-positive patients was 20.5% in the EGF20009 study and 28.5% in the EGF100151 study. In both studies, there was no statistically significant difference in progression-free survival, clinical benefit rate, and overall response rate between p95HER2-positive and p95HER2-negative tumors.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Conclusions:&lt;/b&gt; Lapatinib as a monotherapy or in combination with capecitabine seems to be equally effective in patients with p95HER2-positive and p95HER2-negative HER2-positive breast tumors. Clin Cancer Res; 16(9); 2688–95. ©2010 AACR.&lt;/p&gt;&lt;/div&gt;
Distribution of Isolates of Sclerotium rolfsii Tolerant to Pentachloronitrobenzene in Texas Peanut Fields (1998)

The tolerance to pentachloronitrobenzene (PCNB) of an isolate of Sclerotium rolfsii collected in 1985 was quantified, and a survey of tolerance to PCNB in 377 other isolates of the fungus collected from Texas peanut fields from 1990 through 1994 was conducted. The effective dose (ED)<sub>50</sub> of the previously collected PCNB-tolerant isolate was 11.07 μg PCNB/ml and was more than 5-fold greater than the ED<sub>50</sub> of PCNB-sensitive isolates. The distribution of tolerance to PCNB among all isolates was slightly skewed, with 18 of the 377 isolates identified as having greater (P ≤ 0.05) tolerance to PCNB than the standard sensitive isolate. No isolate of S. rolfsii collected during the period of 1990 to 1994 had as high an ED<sub>50</sub> value as did the 1985 isolate, even among those isolates collected from the same field from which the 1985 isolate was collected. ED<sub>50</sub> values of two PCNB-sensitive and five PCNB-tolerant isolates were unchanged after 15 generations on potato dextrose agar amended with 10 μg PCNB/ml or on unamended media. The PCNB-tolerant isolate collected in 1985 was less aggressive than other isolates in greenhouse and microplot tests, but no correlation was observed between ED<sub>50</sub> values and disease incidence in these tests for other PCNB-sensitive and tolerant isolates. These data suggest that even though high levels of tolerance to PCNB can be confirmed in some isolates of S. rolfsii, this phenomenon is likely to remain a rare event.
Suppplementary Information from Combining Local Immunotoxins Targeting Mesothelin with CTLA-4 Blockade Synergistically Eradicates Murine Cancer by Promoting Anticancer Immunity (2023)

&lt;p&gt;Fig S1. Description of DNA constructs Fig S2. Distribution of intra-tumoral injected trypan blue Fig S3. Combination of intra-tumoral immunotoxins with anti-CTLA-4 is well tolerated by mice. Fig S4. Anti-tumor effect of anti-CTLA-4 and LMB-100 depends on CD8+ cells. Fig S5. High dose of SS1P is needed for induction of complete remission. Fig S6A &amp; B. Anti-tumor effect of an immunotoxin targeting human CD22 &amp; (B) Combination of SS1P with anti-CTLA-4 lead to tumor regressionof 66C14 tumors not expressing MSLN. Fig S7. Intra-tumors injection of paclitaxel does not improve the anti-tumor activity of anti-CTLA-4. Table S1. Combination of RIT and anti-CTLA-4 induces long-term anti-tumor immunity.&lt;/p&gt;
Next generation mm-Wave wireless backhaul based on LOS MIMO links (2016)

With the evolution of the mobile networks towards more dense and flexible configurations, novel wireless backhaul solutions that can match the high capacity and flexibility demands are required in addition to the fixed fiber optics solutions. In this work we analyze the core backhaul requirements and the related system design challenges for utilizing the mm-wave frequency band due to the large available bandwidth. We determine the key performance metrics in terms of achievable throughput and energy efficiency of several transmission schemes seeking for a viable solution for the small cell backhaul scenario. Finally, an insight to the structure of the baseband domain required for processing multiple transmitted streams at the full system bandwidth is presented for a proposed LOS SM-MIMO system concept.
A Fluorogenic Dye Activated by the Staudinger Ligation (2003)

Specific labeling of biomolecules with biochemical and biophysical probes is a central element of proteomics research. Here we describe a coumarin-phosphine dye that undergoes activation of coumarin fluorescence upon Staudinger ligation with azides. Since azides can be metabolically incorporated into cellular proteins and oligosaccharides, this dye may be a useful tool for profiling proteins and their posttranslational modifications.
ChemInform Abstract: Kinetics and Mechanism of the Cyclization of Substituted N‐Phenyl‐2‐ methyl‐2‐(2‐aminophenyl)propanamides and Analogues. (1995)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Distinction among Neuronal Subtypes of Voltage-Activated Sodium Channels by μ-Conotoxin PIIIA (2000)

The functional properties of most sodium channels are too similar to permit identification of specific sodium channel types underlying macroscopic current. Such discrimination would be particularly advantageous in the nervous system in which different sodium channel family isoforms are coexpressed in the same cell. To test whether members of the mu-conotoxin family can discriminate among known neuronal sodium channel types, we examined six toxins for their ability to block different types of heterologously expressed sodium channels. PIIIA mu-conotoxin blocked rat brain type II/IIA (rBII/IIA) and skeletal muscle sodium current at concentrations that resulted in only slight inhibition of rat peripheral nerve (rPN1) sodium current. Recordings from variant lines of PC12 cells, which selectively express either rBII/IIA or rPN1 channel subtypes, verified that the differential block by PIIIA also applied to native sodium current. The sensitivity to block by PIIIA toxin was then used to discriminate between rBII/IIA and rPN1 sodium currents in NGF-treated PC12 cells in which both mRNAs are induced. During the first 24 hr of NGF-treatment, PN1 sodium channels accounted for over 90% of the sodium current. However, over the ensuing 48 hr period, a sharp rise in the proportion of rBII/IIA sodium current occurred, confirming the idea, based on previous mRNA measurements, that two distinct sodium channel types appear sequentially during neuronal differentiation of PC12 cells.
Chiral Separation of Underivatized Amino Acids by Reactive Extraction with Palladium−BINAP Complexes (2009)

In answer to the need for a more economic technology for the separation of racemates, a novel system for reactive enantioselective liquid-liquid extraction (ELLE) is introduced. Palladium (S)-BINAP complexes are employed as hosts in the separation of underivatized amino acids. The system shows the highest selectivity for the ELLE of tryptophan with metal complexes as hosts reported to date and shows a good selectivity toward a range of natural and unnatural amino acids. Furthermore, the host can be prepared in situ from commerically available compounds. Bulk-membrane transport in the form of U-tube experiments demonstrates the enantioselective and catalytic nature of the transport. The dependency of the system on parameters such as pH, organic solvent, and host-substrate ratio has been established. (31)P NMR spectroscopy has been used to confirm the preferred enantiomer in the extraction experiments. The intrinsic selectivity was deduced by determination of the association constants of the palladium complex with the tryptophan enantiomers.
Neutron Diffraction and Electrochemical Studies of Na0.79CoO2and Na0.79Co0.7Mn0.3O2Cathodes for Sodium-Ion Batteries (2014)

Na0.79CoO2 and Na0.79Co0.7Mn0.3O2 with a layered hexagonal structure (P2-type) were synthesized by the Pechini process followed by heat-treatment at elevated temperatures in order to achieve the crystalline phases. The samples were characterized with X-ray diffraction, neutron diffraction, magnetic measurements and electrochemical charge-discharge cycling. X-ray diffraction confirmed the presence of P2 layered hexagonal structure after heat-treatment at 900°C in air. Neutron diffraction patterns confirm Mn substitution on Co sites without forming pronounced Mn-Co ordering. Cyclic voltammetry showed the oxidation and reduction peaks of Co and Mn, indicating the intercalation and de-intercalation behavior of the Na ions. A discharge capacity of 60 mAh/g was achieved for both compositions, with the Na0.79Co0.70Mn0.3O2 composition showing a more stable discharge capacity up to 60 cycles.
Synthesis and Biological Evaluation of N6-Cycloalkyl Derivatives of 1-Deazaadenine Nucleosides: A New Class of Anti-Human Immunodeficiency Virus Agents (1995)

A series of 1-deazaadenine nucleosides with the N6 nitrogen unsubstituted or bearing methyl or cycloalkyl substituents, with or without a chloro group in the 2-position, and with the glycosylic moiety being ribose (1-16), 2'-deoxyribose (17-32), or 2', 3'-dideoxyribose (33-48) were designed and synthesized starting from 5,7-dichloro-3H-imidazo[4,5-b] pyridine (50). These compounds were evaluated for their in vitro activity against human immunodeficiency virus type-1 (HIV-1) and herpes simplex virus type-1 (HSV-1). In addition they were tested for their ability to inhibit adenosine deaminase (ADA) from calf intestine. While the parent compounds 1-deazaadenosine (9), 2'-deoxy-1-deazaadenosine (25), and 2',3'-dideoxy-1- deazaadenosine (41) and the corresponding 2-chloro derivatives were inactive, nucleosides bearing cycloalkyl substituents on N6 exhibited moderate to good anti-HIV-1 activity, compared to 2',3'-dideoxyadenosine, with the degree and pattern of improvement depending on the structure of the sugar moiety. In general, 2'-deoxy- and 2',3'-dideoxy derivatives were more potent compounds than the corresponding ribose nucleosides. Compounds bearing a 6-cycloheptyl or cyclooctylamine were the most active in every series. The presence of a chloro group in the 2-position improved both activity and therapeutic index in every series, the most active compound being 2'-deoxy-2-chloro-N6-cycloheptyl-1-deazaadenosine (23; ED50 = 0.2 microM). On the other hand, most of these derivatives were inactive as anti-HSV-1 agents, showing a high degree of virus selectivity. The 1-deazaadenine derivatives were not substrates of adenosine deaminase, and some of them proved to be good inhibitors of the enzyme. However, the ADA inhibitory activity does not account for the antiviral potency since increased lipophilicity and steric hindrance of substituents resulted in derivatives much less active than the parent compounds.
DifferentialAspergillus lentulusEchinocandin Susceptibilities Are Fksp Independent (2010)

ABSTRACT The recently described species Aspergillus lentulus exhibits differential and reduced susceptibilities to echinocandins and other antifungal drugs in vitro. A. lentulus isolates overall are less susceptible to caspofungin, although they maintain susceptibility to anidulafungin and micafungin. Mutations or polymorphisms in fks , the gene encoding the catalytic subunit of β-1,3-glucan synthase, are known to confer decreased susceptibility to echinocandins in Candida spp. and Aspergillus fumigatus . The analysis of the A. lentulus fks sequence did not reveal a polymorphism at any of the known hot-spot regions of the gene. Caspofungin and micafungin kinetic inhibition profiles of the A. lentulus glucan synthase were comparable to those from susceptible A. fumigatus enzymes. Although the basal cell wall chitin levels in A. lentulus averaged 60% of those in A. fumigatus , echinocandin treatment promoted the increase of cell wall chitin in both organisms, indicating that A. lentulus displays a compensatory chitin response similar to that of A. fumigatus . The data suggest that differential echinocandin susceptibilities in A. lentulus are independent of the echinocandin target, Fksp, and they emphasize the potential that the drugs' capacity to inhibit the target enzyme is unequal at the cellular level.