gebhart/patrepeval huggingface dataset:
Healing of a Large Long-Bone Defect through Serum-Free In Vitro Priming of Human Periosteum-Derived Cells (2017)

Clinical translation of cell-based strategies for regenerative medicine demands predictable in vivo performance where the use of sera during in vitro preparation inherently limits the efficacy and reproducibility. Here, we present a bioinspired approach by serum-free pre-conditioning of human periosteum-derived cells, followed by their assembly into microaggregates simultaneously primed with bone morphogenetic protein 2 (BMP-2). Pre-conditioning resulted in a more potent progenitor cell population, while aggregation induced osteochondrogenic differentiation, further enhanced by BMP-2 stimulation. Ectopic implantation displayed a cascade of events that closely resembled the natural endochondral process resulting in bone ossicle formation. Assessment in a critical size long-bone defect in immunodeficient mice demonstrated successful bridging of the defect within 4 weeks, with active contribution of the implanted cells. In short, the presented serum-free process represents a biomimetic strategy, resulting in a cartilage tissue intermediate that, upon implantation, robustly leads to the healing of a large long-bone defect.
Whole-Mount In Situ Hybridization for Developing Chick Embryos Using Digoxygenin-Labeled RNA Probes (2003)

The design of recombinant gene constructs has proven to be a powerful technique in the field of developmental biology, and has allowed the investigator to analyze the regulation of expression and the function of specific genes during embryonic development. By mapping and correlating the spatiotemporal profile of gene expression in the developing embryo with events of morphogenesis and growth, useful information may be gathered on the functional role of specific genes.
Nanoisland‐Based Random Activation of Fluorescence for Visualizing Endocytotic Internalization of Adenovirus (2010)

Super-resolution imaging is performed on metallic nanoislands by plasmonic-based activation of random near-field hot spots. Nanoislands can be synthesized to create small hot spots that spatially distinguish molecular events on the nanometer scale. Enhanced resolution is experimentally confirmed with fluorescent nanobeads, and is extended to imaging the transport of an adenovirus across a live cell membrane.
Human POT1 disrupts telomeric G-quadruplexes allowing telomerase extension in vitro (2005)

The POT1 (protection of telomeres 1) protein binds the ssDNA overhangs at the ends of chromosomes in diverse eukaryotes. POT1 is essential for chromosome end-protection, as best demonstrated in fission yeast. In human cells, hPOT1 is also involved in telomere-length regulation. We now show that telomeric oligonucleotides, such as d[GGG(TTAGGG)(3)], which form intramolecular G-quadruplexes through Hoogsteen base-pairing, serve as only marginal primers for extension by recombinant human telomerase; telomerase stalls after every nucleotide addition. Addition of hPOT1 to the reaction restores the normal processive elongation pattern seen with primers that cannot form G-quadruplexes. hPOT1 does not act catalytically but, instead, forms a stoichiometric complex with the DNA, freeing its 3' tail. An antisense oligonucleotide, which base-pairs near the 5' end of the telomeric sequence, leaving a telomerase-extendable 3' tail, duplicates the effect of hPOT1 on activation of G-quadruplex primers. Thus, hPOT1 may function simply by trapping the unfolded forms of these telomeric primers in an equilibrium population. We propose an additional role for hPOT1 in telomere maintenance: disrupting G-quadruplex structures in telomeric DNA, thereby allowing proper elongation by telomerase.
Large Block CLOCK (LB-CLOCK): A write caching algorithm for solid state disks (2009)

Solid state disks (SSDs) using NAND flash memory are increasingly being adopted in the high-end servers of datacenters to improve performance of the I/O-intensive applications. Compared to the traditional enterprise class hard disks, SSDs provide faster read performance, lower cooling cost, and higher power efficiency. However, write performance of a flash based SSD can be up to an order of magnitude slower than its read performance. Furthermore, frequent write operations degrade the lifetime of flash memory. A nonvolatile cache can greatly help to solve these problems. Although a RAM cache is relative high in cost, it has successfully eliminated the performance gap between fast CPU and slow magnetic disk. Similarly, a nonvolatile cache in an SSD can alleviate the disparity between the flash memory's read and write performance. A small write cache that reduces the number of flash block erase operations, can lead to substantial performance gain for write-intensive applications and can extend the overall lifetime of flash based SSDs. This paper presents a novel write caching algorithm, the Large Block CLOCK (LB-CLOCK) algorithm, which considers `recency' and `block space utilization' metrics to make cache management decisions. LB-CLOCK dynamically varies the priority between these two metrics to adapt to changes in workload characteristics. Our simulation based experimental results show that LB-CLOCK outperforms the best known existing flash caching algorithms for a wide range of workloads.
A Modelling and Optimisation Management Tool for large-scale supply chain networks (2009)

Supply Chains (SC) can be complex, dynamic and large-scale. They can make modelling and optimisation of SC difficult tasks. This paper describes a framework of a new Modelling and Optimisation Management Tool (MOMT) for large-scale supply chain networks. A graph-based method is used to automatically generate and update optimisation models for large-scale SC networks. Graph's nodes and arcs, that define the optimisation model's constraints and variables, are generated according to the SC requirements. MOMT increases accuracy and flexibility of modelling and information processing in large-scale SC models. The tool applications, advantages and disadvantages are described by real world examples from Motorola Inc. SC.
Allergy-Preventive Phenolic Glycosides from Populus sieboldii (2006)

Allergy-preventive activity was demonstrated for an extract of the bark of Populus sieboldii in a continuing search for allergy-preventive substances from natural sources. By bioassay-directed fractionation of this plant bark, two new phenolic glycosides, siebolside A {2-hydroxy-5-[(benzoyloxy)methyl]phenyl (6'-O-acetyl) beta-D-glucopyranoside} (1) and siebolside B {2-hydroxy-5-[(benzoyloxy)methyl]phenyl beta-D-glucopyranoside} (2), were isolated, together with three known compounds, salicin (3), sakuranin (4), and neosakuranin (5). The structures of 1 and 2 were elucidated by spectroscopic methods. Compounds 1-5 all showed allergy-preventive effects.
THE QUORUM-QUENCHING LACTONASE FROM BACILLUS THURINGIENSIS IS A METALLOPROTEIN (2005)

Lactonases from Bacillus species hydrolyze the N-acylhomoserine lactone (AHL) signaling molecules used in quorum-sensing pathways of many Gram-negative bacteria, including Pseudomonas aeruginosa and Erwinia carotovora, both significant pathogens. Because of sequence similarity, these AHL lactonases have been assigned to the metallo-beta-lactamase superfamily of proteins, which includes metalloenzymes of diverse activity, mechanism, and metal content. However, a recent study claims that AHL lactonase from Bacillus sp. 240B1 is not a metalloprotein [Wang, L. H., et al. (2004) J. Biol. Chem. 279, 13645]. Here, the gene for an AHL lactonase from Bacillus thuringiensis is cloned, and the protein is expressed, purified, and found to bind 2 equiv of zinc. The metal-bound form of AHL lactonase catalyzes the hydrolysis of N-hexanoyl-(S)-homoserine lactone but not the (R) enantiomer. Removal of both zinc ions results in loss of activity, and reconstitution with zinc restores activity, indicating the importance of metal ions for catalytic activity. Metal content, sequence alignments, and X-ray absorption spectroscopy of the zinc-containing lactonase all support a proposed dinuclear zinc binding site similar to that found in glyoxalase II.
In Vivo Treatment of Class II MHC-Deficient Mice with Anti-TCR Antibody Restores the Generation of Circulating CD4 T Cells and Optimal Architecture of Thymic Medulla (2003)

TCR ligation by the self-peptide-associated MHC molecules is essential for T cell development in the thymus, so that class II MHC-deficient mice do not generate CD4(+)CD8(-) T cells. The present results show that the administration of anti-TCR mAb into class II MHC-deficient mice restores the generation of CD4(+)CD8(-) T cells in vivo. The CD4 T cells were recovered in the thymus, peripheral blood, and the spleen, indicating that the anti-TCR treatment is sufficient for peripheral supply of newly generated CD4 T cells. Unlike peripheral CD4 T cells that disappeared within 5 wk after the treatment, CD4(+)CD8(-) thymocytes remained undiminished even after 5 wk, suggesting that CD4 T cells in the thymus are maintained separately from circulating CD4 T cells and even without class II MHC molecules. It was also found that the mass of medullary region in the thymus, which was reduced in class II MHC-deficient mice, was restored by the anti-TCR administration, suggesting that the medulla for CD4(+)CD8(-) thymocytes is formed independently of the medulla for CD4(-)CD8(+) thymocytes. These results indicate that in vivo anti-TCR treatment in class II MHC-deficient mice restores the generation of circulating CD4 T cells and optimal formation of the medulla in the thymus, suggesting that anti-TCR Ab may be useful for clinical treatment of class II MHC deficiencies.
GALS for Bursty Data Transfer based on Clock Coupling (2009)

In this paper we introduce a novel burst-mode GALS technique. The goal of this technique is improving the performance of the GALS approach for systems with predominantly bursty data transfer. This new technique has been used to implement a GALS-based version of a hardware accelerator of a 60 GHz OFDM baseband processor. The simulation results show a significant performance improvement in comparison with a classical implementation of GALS using pausible clocking.
A multicenter, phase II study of maintenance azacitidine in older patients with acute myeloid leukemia in complete remission after induction chemotherapy (2015)

Older patients with acute myeloid leukemia (AML) have poor outcomes, with median durations of complete remission lasting less than 1 year. Increased toxicity in older patients limits the delivery of standard consolidation therapies, such as allogeneic stem cell transplant or high-dose cytarabine. Azacitidine, a nucleoside analog/DNA methyltransferase inhibitor, has demonstrated significant activity and favorable tolerability in patients unable to tolerate intensive induction chemotherapy; however, the role of azacitidine in the maintenance setting has not been fully evaluated. We undertook a pilot study of low-dose subcutaneous azacitidine [50 mg/(m(2) day)] for 5 days every 4 weeks) in AML patients ≥60 years of age in first remission following standard induction therapy. The primary objective was to determine the 1-year disease-free survival (DFS); secondary objectives were to determine safety and tolerability. We enrolled 24 patients (median age 68, range 62-81 years), the majority of whom received anthracycline-cytarabine induction regimens. From the time of first complete remission, the estimated 1-year DFS was 50% and the median overall survival was 20.4 months. Thrombocytopenia and neutropenia were the most common grade 3/4 toxicities (50 and 58%, respectively). In our study population, maintenance therapy with subcutaneous azacitidine was safe and well tolerated.
Acid softness and hardness in large-pore zeolites as a determinant parameter to control selectivity in orbital-controlled reactions (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAcid softness and hardness in large-pore zeolites as a determinant parameter to control selectivity in orbital-controlled reactionsA. Corma, F. LLopis, P. Viruela, and C. Zicovich-WilsonCite this: J. Am. Chem. Soc. 1994, 116, 1, 134–142Publication Date (Print):January 1, 1994Publication History Published online1 May 2002Published inissue 1 January 1994https://pubs.acs.org/doi/10.1021/ja00080a016https://doi.org/10.1021/ja00080a016research-articleACS PublicationsRequest reuse permissionsArticle Views320Altmetric-Citations49LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Mobile UNITY (1997)

Mobile computing represents a major point of departure from the traditional distributed-computing paradigm. The potentially very large number of independent computing units, a decoupled computing style, frequent disconnections, continuous position changes, and the location-dependent nature of the behavior and communication patterns present designers with unprecedented challenges in the areas of modularity and dependability. So far, the literature on mobile computing is dominated by concerns having to de with the development of protocols and services. This article complements this perspective by considering the nature of the underlying formal models that will enable us to specify and reason about such computations. The basic research goal is to characterize fundamental issues facing mobile computing. We want to achieve this in a manner analogous to the way concepts such as shared variables and message passing help us understand distributed computing. The pragmatic objective is to develop techniques that facilitate the verification and design of dependable mobile systems. Toward this goal we employ the methods of UNITY. To focus on what is essential, we center our study on ad hoc networks , whose singular nature is bound to reveal the ultimate impact of movement on the way one computes and communicates in a mobile environment. To understand interactions we start with the UNITY concepts of union and superposition and consider direct generalizations to transient interactions. The motivation behind the transient nature of the interactions comes from the fact that components can communicate with each other only when they are within a a certain range. The notation we employ is a highly modular extension of the UNITY programming notation. Reasoning about mobile computations relies on extensions to the UNITY proof logic.
Ataxia-telangiectasia–Mutated Protein Kinase Levels Stratify Patients With Pancreatic Adenocarcinoma Into Prognostic Subgroups With Loss Being a Strong Indicator of Poor Survival (2014)

Our analysis shows that both ATM expression and activated ATM are prognostic markers in PDAC with respect to standard clinicopathological parameters. These results suggest that ATM should be further explored as prognostic as well as predictive factor with respect to conventional chemotherapies and for putative synthetic lethal approaches.
28‐4L: Late‐News Paper : An Ultra High Density 1.96”UHD 2250ppi Display (2017)

We developed the world's highest resolution panel (2250ppi, 1.96 "UHD) with glass‐based process technology. A new pixel design and architecture has been devised to integrate TFT, contact hole, and data / gate lines in a small pixel pitch. A small TFT has been implemented and an extreme process has been developed. These technologies are expected to be applied mainly to VR, AR and Hologram platform.
Attack Modeling Methodology and Taxonomy for Intelligent Transportation Systems (2021)

With newer technologies, the embedded hardware and software in traditional vehicles and traffic control infrastructure continue to become more interconnected and more vulnerable. To assist in dealing with existing and potential vulnerabilities, we present a novel attack modeling methodology, taxonomy, and metrics (relative average waiting time, average network flow, impacts and rate of changes) to model, simulate, and meaningfully evaluate the security of Intelligent Transportation Systems. We implement our work in two different architectures: 1) Newell’s Car-Following Model with Bounded Acceleration (the BA-Newell Model) in Matlab and 2) Intelligent Driver Model in Veins. Our code is entirely open-sourced and will be maintained so that the ITS community may use it as a tool. We observe that the architectural-related metric values for sample attack simulation results are similar and transferable; where, for example, the rate of change values have range of average distances 1.8-3.5% for network flow impact and 3.3-9.6% for wait time impact.
Enantioselective Preparation of Arenes with β‐Stereogenic Centers: Confronting the 1,1‐Disubstituted Olefin Problem Using CuH/Pd Cooperative Catalysis (2020)

Arenes with β-stereogenic centers are important substructures in pharmaceuticals and natural products. We have developed an asymmetric anti-Markovnikov hydroarylation of 1,1-disubstituted olefins by dual palladium and copper hydride catalysis as a convenient and general approach to access these substructures. This efficient one-step process addresses several limitations of the traditional stepwise approaches. The use of cesium benzoate as a base and a common phosphine ligand for both the Cu- and Pd-catalyzed processes were important discoveries that allow these challenging olefin substrates to be efficiently transformed. A variety of aryl bromide coupling partners, including numerous heterocycles, were coupled with 1,1-disubstituted alkenes to generate arenes with β-stereogenic centers.
Thermal Isotropic Atomic Layer Etching of Metallic Molybdenum (2020)

In high aspect ratio structures such as 3D NAND stacks, high-k oxides or certain metals need to be removed from sidewalls and even non-line-of-site locations where access via reactive ion etching would be challenging or impossible. To achieve etching in these locations, thermal processes need to be applied that lack any intrinsic directionality and are therefore isotropic. Thermal Atomic Layer Etching is one option to facilitate this kind of process. Therefore, it has gathered significant attention recently and is now being explored in academia and industry. In this work, we demonstrate isotropic Atomic Layer Etching on metallic molybdenum using a three-step process that involves oxygenation of the metal surface, a conversion reaction using boron-trichloride followed by a hydrogen-fluoride clean step. We measured the etch rate per cycle to be around 10 Å with low temperature dependence in the range between 150°C and 250°C. Furthermore, we examined the effectiveness of various oxygenation methods on the surface of metallic molybdenum films. These methods included thermal oxygenation as a function of temperature, oxygenation by a remote plasma and in-situ plasma fluorination. We established that an oxygenation level of more than 70% as measured with XPS is needed in order for the ALE process to work. Levels in this range could be achieved via in-situ plasma oxygenation as well as with a remote plasma. Thermal oxygenation, on the other hand, required substrate temperatures in access of 400°C to oxygenate at the 70% level. In this study we also show an example of molybdenum ALE in an application that involved the removal of this metal from a 50:1 aspect ratio 3D NAND structure. We measured etch rates on vertical and horizontal surfaces as well as the depth dependence in removal rate of molybdenum.
Anti-Stokes photoluminescence from LiY1-xYbxF4 nanocrystals (2022)

We have successfully synthesized lithium yttrium fluoride (YLF) nanocrystals doped with ytterbium. The Yb content was varied between 1% and 10%. The nanocrystals emit in near-infrared, with the emission spectrum extending from 960 nm to 1060 nm when excited with the 900 nm light. Strong anti-Stokes photoluminescence was observed when using excitation wavelengths ranging from 1010 nm to 1020 nm. The temperature-dependence of the anti-Stokes photoluminescence was measured over the range from 10 °C till 70 °C. These nanocrystals have a high potential to be used in optical cooling applications.
Cross-coupling reactions in Cinchona alkaloid chemistry: aryl-substituted and dimeric quinine, quinidine, as well as quincorine and quincoridine derivatives (2001)

Cross-coupling reactions of modified Cinchona alkaloids provide access to a wide variety of novel arylated and dimeric derivatives of quinine and quinidine containing a single and double 1,2-amino alcohol functionality. Sonogashira and Heck reactions allow functionalization of ethynyl and 11-iodovinyl precursors. The role of bystander functionality is investigated.
2P-083 Thermodynamic parameters of interaction between SMR-type multidrug transporter and its substrates(Membrane proteins,The 47th Annual Meeting of the Biophysical Society of Japan) (2009)

TheBiophysicalSociety of
Welcome (2015)

The Transducers Conference series began in Boston in 1981, and the subsequent series of biennial Conferences were held in Delft, Philadelphia, Tokyo, Montreux, San Francisco, Yokohama, Stockholm, Chicago, Sendai, Munich, Seoul, Lyon, Denver, Beijing, and Barcelona, rotating through Americas, Asia/Oceania, and Europe/Africa. This is the first time the Transducers Conference will be held in Alaska.
Large-area optoelectronic-grade InSe thin films via controlled phase evolution (2020)

Indium monoselenide (InSe) is an emerging two-dimensional semiconductor with superlative electrical and optical properties whose full potential for high-performance electronics and optoelectronics has been limited by the lack of reliable large-area thin-film synthesis methods. The difficulty in InSe synthesis lies in the complexity of the indium-selenium phase diagram and inadequate understanding of how this complexity is manifested in the growth of thin films. Herein, we present a systematic method for synthesizing InSe thin films by pulsed laser deposition followed by vacuum thermal annealing. The controlled phase evolution of the annealed InSe thin films is elucidated using a comprehensive set of in situ and ex situ characterization techniques. The annealing temperature is identified as the key parameter in controlling phase evolution with pure thin films of InSe developed within a window of 325 °C to 425 °C. To exert finer stoichiometric control over the as-deposited InSe thin film, a co-deposition scheme utilizing InSe and In2Se3 pulsed laser deposition targets is employed to mitigate the effects of mass loss during annealing, ultimately resulting in the synthesis of centimeter-scale, thickness-tunable ε-InSe thin films with high crystallinity. The optimized InSe thin films possess a strong optoelectronic response, exhibited by phototransistors with high responsivities up to 103 A/W. Additionally, enhancement-mode InSe field-effect transistors are fabricated over large areas with device yields exceeding 90% and high on/off current modulation greater than 104, realizing a degree of electronic uniformity previously unattained in InSe thin-film synthesis.