gebhart/patrepeval huggingface dataset:
KV7 channels contribute to paracrine, but not metabolic or ischemic, regulation of coronary vascular reactivity in swine (2016)

Hydrogen peroxide (H2O2) and voltage-dependent K(+) (KV) channels play key roles in regulating coronary blood flow in response to metabolic, ischemic, and paracrine stimuli. The KV channels responsible have not been identified, but KV7 channels are possible candidates. Existing data regarding KV7 channel function in the coronary circulation (limited to ex vivo assessments) are mixed. Thus we examined the hypothesis that KV7 channels are present in cells of the coronary vascular wall and regulate vasodilation in swine. We performed a variety of molecular, biochemical, and functional (in vivo and ex vivo) studies. Coronary arteries expressed KCNQ genes (quantitative PCR) and KV7.4 protein (Western blot). Immunostaining demonstrated KV7.4 expression in conduit and resistance vessels, perhaps most prominently in the endothelial and adventitial layers. Flupirtine, a KV7 opener, relaxed coronary artery rings, and this was attenuated by linopirdine, a KV7 blocker. Endothelial denudation inhibited the flupirtine-induced and linopirdine-sensitive relaxation of coronary artery rings. Moreover, linopirdine diminished bradykinin-induced endothelial-dependent relaxation of coronary artery rings. There was no effect of intracoronary flupirtine or linopirdine on coronary blood flow at the resting heart rate in vivo. Linopirdine had no effect on coronary vasodilation in vivo elicited by ischemia, H2O2, or tachycardia. However, bradykinin increased coronary blood flow in vivo, and this was attenuated by linopirdine. These data indicate that KV7 channels are expressed in some coronary cell type(s) and influence endothelial function. Other physiological functions of coronary vascular KV7 channels remain unclear, but they do appear to contribute to endothelium-dependent responses to paracrine stimuli.
Use of electron beam lithography to selectively decompose metalorganics into patterned thin-film superconductors (1988)

Fine line superconductors, approximately 5 μm in width and 260 nm thick, were formed from Y-Ba-Cu on 〈100〉SrTiO3 by the combined methods of metalorganic deposition and selective area electron beam exposure. The lines were written in metal neodecanoates using an electron beam having a spot size of 0.25 μm and an energy of 25 kV. The dosage of the exposure was 1200 μC/cm2. Unexposed areas were removed with a 30 s xylene wash. A 500 °C pyrolysis in air for 300 s followed by rapid thermal annealing in oxygen produced lines having superconducting onsets above 90 K and zero resistance at 69 K.
SIMULTANEOUS ANALYSIS OF VITAMIN C AND ASPIRIN IN ASPIRIN C EFFERVESCENT TABLETS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY–PHOTODIODE ARRAY DETECTOR (2012)

High performance liquid chromatography–photodiode array (HPLC–PDA) method has been employed for the simultaneous determination of vitamin C (ascorbic acid) and aspirin (acetylsalicylic acid) in aspirin C effervescent tablets. Chromatographic separation of ascorbic acid (AA) and acetylsalicylic acid (ASA) was performed by reversed–phase using a Betasil C18 column with particle size of 3 µm, 150 mm × 4.6 mm I.D. Optimum separation was achieved in isocratic mode with a binary mobile phase (mixture of water and acetonitrile with 0.1% of formic acid, 75:25, v/v) at a flow rate of 1.0 mL min−1. Recovery of the target compounds was obtained more than 98% with good quality parameters: linearity (r 2 > 0.997), limit of detection (LOD) and limit of quantification (LOQ) values between 1.4 × 10−3 and 5.1 × 10−4 mg mL−1, and intra-day and interday precisions with relative standard deviation (RSD) lower than 2.1%. The content of the analyzed samples ascorbic and acetylsalicylic acid was found 238.23 and 393.49 mg, respectively, which are very close to the label claimed by the manufacturer in aspirin C effervescent tablets. Therefore, this method can be proposed for routine analysis of these compounds in aspirin C effervescent tablets.
<title>Optimal tree approximation with wavelets</title> (1999)

The more a priori knowledge we encode into a signal processing algorithm, the better performance we can expect. In this paper, we overview several approaches to capturing the structure of singularities (edges, ridges, etc.) in wavelet-based signal processing schemes. Leveraging results from approximation theory, we discuss nonlinear approximations on trees and point out that an optimal tree approximant exists and is easily computed. The optimal tree approximation inspires a new hierarchical interpretation of the wavelet decomposition and a tree-based wavelet denoising algorithm that suppresses spurious noise bumps.
Ilaprazole and other novel prazole-based compounds that bind Tsg101 inhibit viral budding of HSV-1/2 and HIV from cells (2020)

Abstract In many enveloped virus families, including HIV and HSV, a crucial, yet unexploited, step in the viral life cycle is releasing particles from the infected cell membranes. This release process is mediated by host ESCRT complex proteins, which is recruited by viral structural proteins and provides the mechanical means for membrane scission and subsequent viral budding. The prazole drug, tenatoprazole, was previously shown to bind to ESCRT complex member Tsg101 and quantitatively block the release of infectious HIV-1 from cells in culture. In this report we show that tenatoprazole and a related prazole drug, ilaprazole, effectively block infectious Herpes Simplex Virus (HSV)-1/2 release from Vero cells in culture. By electron microscopy, we found that both prazole drugs block the release of HSV particles from the cell nuclear membrane resulting in their accumulation in the nucleus. Ilaprazole also quantitatively blocks the release of HIV-1 from 293T cells with an EC 50 of 0.8 μM, which is more potent than tenatoprazole. Finally, we synthesized and tested multiple novel prazole-based analogs that demonstrate both binding to Tsg101 and inhibition of viral egress in the nanomolar range of HIV-1 from 293T cells. Our results indicate that prazole-based compounds may represent a class of drugs with potential to be broad-spectrum antiviral agents against multiple enveloped viruses, by interrupting cellular Tsg101 interaction with maturing virus, thus blocking the budding process that releases particles from the cell. Importance These results provide the basis for the development of drugs that target enveloped virus budding that can be used ultimately to control multiple virus infections in humans.
Anthropic Correction of Information Estimates and Its Application to Neural Coding (2010)

Information theory has been used as an organizing principle in neuroscience for several decades. Estimates of the mutual information (MI) between signals acquired in neurophysiological experiments are believed to yield insights into the structure of the underlying information processing architectures. With the pervasive availability of recordings from many neurons, several information and redundancy measures have been proposed in the recent literature. A typical scenario is that only a small number of stimuli can be tested, while ample response data may be available for each of the tested stimuli. The resulting asymmetric information estimation problem is considered. It is shown that the direct plug-in information estimate has a negative bias. An anthropic correction is introduced that has a positive bias. These two complementary estimators and their combinations are natural candidates for information estimation in neuroscience. Tail and variance bounds are given for both estimates. The proposed information estimates are applied to the analysis of neural discrimination and redundancy in the avian auditory system.
Learning reliable manipulation strategies without initial physical models (2002)

A description is given of a robot, possessing limited sensory and effectory capabilities but no initial model of the effects of its actions on the world, that acquires such a model through exploration, practice, and observation. By acquiring an increasingly correct model of its actions, it generates increasingly successful plans to achieve its goals. In an apparently nondeterministic world, achieving reliability requires the identification of reliable actions and a preference for using such actions. Furthermore, by selecting its training actions carefully, the robot can significantly improve its learning rate.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Embedded binary tree for dynamic point cloud geometry compression with graph signal resampling and prediction (2017)

This paper proposes a temporal prediction scheme for the compression of point cloud sequences with dynamic 3D geometry information. Since the point cloud sequences are non-uniformly sampled signals on a graph, it is difficult to apply traditional prediction schemes in video coding such as motion compensation. Instead, we propose a binary tree with embedded octree coding scheme that allows for motion compensation and residual coding at the level of binary tree leaf node. Graph signal processing based reference block re-sampling is employed to eliminate the need to signal the reference points, and a bipartite graph pruning algorithm is used for residual prediction. Simulation results demonstrate the effectiveness of the proposed scheme in a rate distortion sense.
Scaling 112 Gb/s Optical Networks With the Nonlinear Threshold Metric (2012)

A nonlinear penalty threshold that contains the total nonlinear phase shift is examined as a criterion for scaling fiber optic links. We investigate 112 Gb/s PDM-QPSK hybrid optical networks consisting of either TrueWave (G.655) or AllWave (G.652) fiber. We establish experimental and simulation environments with robust absolute matching in both linear and nonlinear regimes. We identify both XPM and SPM penalties in 0% and 100% inline dispersion compensation schemes for the two different fiber types. Both experimental and simulation results reveal that 0% inline-compensated links operate with larger nonlinear threshold for increasing span count and readily yield simple design rules.
nt i Glucocorticoid Receptor Transcripts Lack Sequences Encoding the Amino-Terminal Transcriptional Modulatory Domain (1990)

Glucocorticoid induction of cell death (apoptosis) in mouse lymphoma S49 cells has long been studied as a molecular genetic model of steroid hormone action. To better understand the transcriptional control of glucocorticoid-induced S49 cell death, we isolated and characterized glucocorticoid receptor (GR) cDNA from two steroid-resistant nti S49 mutant cell lines (S49.55R and S49.143R) and the wild-type parental line (S49.A2). Our data reveal that nti GR transcripts encode intact steroid- and DNA-binding domains but lack 404 amino-terminal residues as a result of aberrant RNA splicing between exons 1 and 3. Results from transient cotransfection experiments into CV1 cells using nti receptor expression plasmids and a glucocorticoid-responsive reporter gene demonstrated that the truncated nti receptor exhibits a reduced transcriptional regulatory activity. Gene fusions containing portions of both the wild-type and the nti GR-coding sequences were constructed and used to functionally map the nti receptor mutation. We found that the loss of the modulatory domain alone is sufficient to cause the observed defect in nti transcriptional transactivation. These results support the proposal that glucocorticoid-induced S49 cell death requires GR sequences which have previously been shown to be required for transcriptional regulation, suggesting that steroid-regulated apoptosis is controlled at the level of gene expression.
High‐Resolution Soft Lithography: Enabling Materials for Nanotechnologies (2004)

Ein guter Eindruck: Photohärtbare, flüssige Perfluorpolyether (PFPEs) eignen sich ideal für hochauflösende (<100 nm) Musterübertragungs- und lithographische Prägeprozesse (siehe Bild). Die PFPEs weisen Merkmale von Elastomeren wie von steifen Materialien auf, haben eine bemerkenswert niedrige Oberflächenenergie, geben sehr kleine Muster mit großer Genauigkeit wieder, werden durch die meisten organischen Solventien nicht gequollen und lassen sich wiederholt in Gießprozessen einsetzen.
Adoption of a focal production innovation within a supply network (2006)

An empirical study using mixed-methods is reported into the barriers and facilitating factors for the diffusion of Digital Inkjet Printing (DIP) as a full-scale production method within the supply network of the textile manufacturing industry. Data are obtained from interviews with a convenience sample of 27 organisations representing the main stakeholder groups in the network. To guide the analysis, a conceptual framework is applied that focuses on four key aspects: knowledge of the technology, perceived characteristics of the technology, network relationships and absorptive capacities associated with three areas of innovation: product, process and administrative. Earlier industry use of DIP to produce samples is shown to play a part in promoting the technology as a full-scale production method. The supply network is not dominated by any particular stakeholder group and anticipated production costs are an important influence on adoption activity.
DOSY NMR and MALDI‐TOF evidence of covalent binding the DNA duplex by trimethylammonium salts of topotecan upon near UV irradiation (2015)

Using DOSY NMR and MALDI-TOF MS techniques, we present evidence that quaternary trimethylammonium salts of topotecan, [TPT-NMe3 ](+) X(-) (X = CF3SO3, HCOO), bind covalently the natural DNA oligomer upon near UV irradiation in water under physiological conditions. It is shown that formate salt is very reactive at pH 7 and requires short irradiation time. This weak irradiation at 365 nm paves the way for a new application of TPT derivatives in clinical use, which can dramatically increase the therapeutic effects of a medicine.
Electrically pumped fluidic cavity (EPFC) VCSEL for the detection of biologic agents (2003)

A fluidic cavity vertical-cavity surface-emitting laser (VCSEL) is presented for the detection of biological agents via introducing the analytic biofluid into the high finesse laser cavity. The optical properties of the fluid as modified by the biological cells they contain are sensed by monitoring the output optical intensity and wavelength of the laser. As a preliminary study, our first generation electrically pumped GaAs/AlGaAs based fluidic cavity VCSEL is described, with emphasis on the system design and techniques for the system construction. The device shows a strong spontaneous emission and a considerable wavelength shift when DI water is capillarily fed into the fluidic cavity.
CCR3 is essential for skin eosinophilia and airway hyperresponsiveness in a murine model of allergic skin inflammation (2002)

The CC chemokine receptor 3 (CCR3) is expressed by eosinophils, mast cells, and Th2 cells. We used CCR3(-/-) mice to assess the role of CCR3 in a murine model of allergic skin inflammation induced by repeated epicutaneous sensitization with ovalbumin (OVA), and characterized by eosinophil skin infiltration, local expression of Th2 cytokines, and airway hyperresponsiveness (AHR) to inhaled antigen. Eosinophils and the eosinophil product major basic protein were absent from the skin of sham and OVA-sensitized CCR3(-/-) mice. Mast cell numbers and expression of IL-4 mRNA were normal in skin of CCR3(-/-) mice, suggesting that CCR3 is not important for infiltration of the skin by mast cells and Th2 cells. CCR3(-/-) mice produced normal levels of OVA-specific IgE, and their splenocytes secreted normal amounts of IL-4 and IL-5 following in vitro stimulation with OVA, indicating effective generation of systemic Th2 helper responses. Recruitment of eosinophils to lung parenchyma and bronchoalveolar lavage (BAL) fluid was severely impaired in CCR3(-/-) mice, which failed to develop AHR to methacholine following antigen inhalation. These results suggest that CCR3 plays an essential role in eosinophil recruitment to the skin and the lung and in the development of AHR.
Supplementary Figure S2 from CMVpp65 Vaccine Enhances the Antitumor Efficacy of Adoptively Transferred CD19-Redirected CMV-Specific T Cells (2023)

&lt;p&gt;Supplementary Figure S2. Phenotypic characterization of different subsets in the T cell products&lt;/p&gt;
Semi-Deterministic Radio Channel Modeling Based on Graph Theory and Ray-Tracing (2016)

Graph theory channel modeling is an efficient approach to simulate multipath radio propagation including the reverberation effect of electromagnetic waves. In this contribution, without modifying the modeling framework, we proposed a semi-deterministic channel modeling approach by associating the scatterers with realistic environment objects, and by calculating the coefficients of the propagation paths on the base of a physically sound and proven diffuse scattering theory. The diffuse multipath components are then combined with the specular components (SCs) simulated by ray-tracing (RT) to obtain a complete channel representation. The proposed method is evaluated in two reference scenarios at 3.8 and 60 GHz, respectively, by comparing the simulated channel characteristics with channel measurement data. Results show that the proposed method can accurately predict the channel characteristics in both the delay domain and the angular domain. The proposed approach is appropriate to model multipath propagation in confined indoor or dense-urban environment at millimeter (mm)-wave frequencies and above, where reverberation and rough-surface scattering can be important phenomena.
Tunable All-Optical WDM Channel Selection using Raman Assisted Cascaded Parametric Amplification (2016)

A cascaded configuration of Parametric Amplifiers (PA) using distributed Raman amplification is proposed to selectively extract either of QPSK channels in a WDM system. Wavelength selectivity is obtained by changing PA pump wavelength and phase.
Theoretical study of collinear Be+FH(v1) →BeF(v2) +H (1978)

The potential energy surface for collinear Be+FH→BeF+H has been studied at various levels of ab initio approximation. A final surface was obtained from a first order configuration interaction wavefunction, using the iterative natural orbital method and a medium-sized basis set of Slater atomic functions; this is expected to give a semiquantitative description of the reactive process. The exothermicity is computed to be 6 kcal/mole which can be compared with the best experimental value of 2±4 kcal/mole. The barrier height is predicted to be 28 kcal/mole at a geometry where both internuclear separations are extended by about 0.4 bohr from their asymptotic equilibrium values. This surface differs qualitatively from simple LEPS models. The curvature of the reaction path is much more abrupt, the atom effecting little distortion of the partner molecule until quite close approach in both entrance and exit channels. The surface was fit with bicubic splines and dynamics was studied by the quasiclassical trajectory method as a function of initial kinetic energy for the reactant initially in v1=0 and v1=1. The reaction probability and final energy distributions were found to depend sensitively and selectively on the initial kinetic and vibrational energy. Most of the available energy is channeled into product translation; for v1=0 at higher initital kinetic energies, less than 10% of the available energy becomes product vibration. Also, addition of reactant vibrational energy has a profound effect on reaction probability and final vibrational distributions. Examination of typical trajectories made it possible to identify the surface features responsible for the dynamical behavior. For comparison, calculations were also done on a LEPS surface constructed to have the same barrier position and height. Because the LEPS surface has a more gently curved reaction path, with better coupling of vibrational and translational energy, it results in less specific energy use and disposal. For example, 40%–50% of the available energy was channeled into product vibration on the LEPS surface, and addition of reactant vibrational energy effected only small changes in the dynamics. These results underline the dangers of using oversimplified potential surfaces in the study of reactive collision dynamics.
Waveform and Beamforming Design for Intelligent Reflecting Surface Aided Wireless Power Transfer: Single-User and Multi-User Solutions (2021)

In this paper, we study the waveform and passive beamforming design for intelligent reflecting surface (IRS)-aided wireless power transfer (WPT). Generalized multi-user and low complexity single-user algorithms are derived based on alternating optimization (AO) framework to maximize the weighted sum output DC current, subject to transmit power constraints and passive beamforming phases unit modulus constraints. The input signal waveform and IRS passive beamforming phase shifts are jointly designed as a function of users' individual frequency-selective channel state information (CSI). The energy harvester nonlinearity is explored and two IRS deployment schemes, namely frequency selective IRS (FS-IRS) and frequency flat IRS (FF-IRS), are modeled and analyzed. This paper highlights the fact that IRS can provide an extra passive beamforming gain on output DC power over conventional WPT designs and significantly influence the waveform design by leveraging the benefit of passive beamforming, frequency diversity and energy harvester nonlinearity. Even though FF-IRS exhibits lower output DC current than FS-IRS, it still achieves substantially increased DC power over conventional WPT designs. Performance evaluations confirm the significant benefits of a joint waveform and passive beamforming design accounting for the energy harvester nonlinearity to boost the performance of single-user and multi-user WPT system.
Wind sensing demonstration of more than 30km measurable range with a 1.5μm coherent Doppler lidar which has the laser amplifier using Er,Yb:glass planar waveguide (2012)

Recently, we have developed the high output power laser amplifier using Er,Yb:glass planar waveguide in order to increase the measurable range of our 1.5 &mu;m coherent Doppler LIDAR (CDL). In this paper, we introduce this development and demonstration of a long range wind sensing using the developed system. The transmitted pulse has a peak power of 2.4 kW and a width of 580 ns (i.e. pulse energy of 1.4 mJ) with a pulse repetition frequency of 4 kHz, in addition to a nearly diffraction limited beam quality. With this laser amplifier, we demonstrate the measurable range of more than 30 km. According to our own research, this is the longest measurable range demonstration for wind sensing CDLs.
First Complete Genome Sequence of an Entomopathogenic Nematode Goes Public (2013)

Entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) have a fascinating biology as they form mutualistic&#13;\nsymbioses with bacteria (Enterobacteriacae) and together they serve as important biological control agents of insect&#13;\npests. Here we reveal the first complete genome sequence of an athogenic nematode species that will serve as a model for&#13;\nfuture genetic studies in this group and in the phylum Nematoda.
Metal Oxides (2010)

Abstract This article details the preparation, catalytic activity, computer modeling, and design of heterogeneous oxide catalysts.
Mono‐ and Dinuclear Ruthenium Complexes for Nanocrystalline TiO2 Based Dye‐Sensitized Photovoltaics (2003)

Abstract We have carried out synthesis, characterization, and theoretical modeling of a rigid, rod‐like homometallic dinuclear ruthenium complex and an analogous mononuclear ruthenium complex using terpyridine and bipyridine ligands. The photophysical and photovoltaic properties have been investigated. We compared the results to understand the charge transfer processes in these complexes. The dinuclear complex showed overall photoelectric conversion efficiency of 1.9% in dye‐sensitized nanocrystalline TiO2 based photovoltaic cells under simulated AM1.5 solar irradiation and is found to be an efficient photosensitizer compared to the mononuclear complex.