gebhart/patrepeval huggingface dataset:
Synthesis of 4,5-dihydroazepine-3,6-dicarboxylate derivatives by stereoselective ring expansion-nucleophilic addition to 4-.alpha.-chloroalkyl-1,4-dihydropyridine-3,5-dicarboxylates (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleSynthesis of 4,5-dihydroazepine-3,6-dicarboxylate derivatives by stereoselective ring expansion-nucleophilic addition to 4-.alpha.-chloroalkyl-1,4-dihydropyridine-3,5-dicarboxylatesDavid A. Claremon, David E. McClure, James P. Springer, and John J. BaldwinCite this: J. Org. Chem. 1984, 49, 20, 3871–3874Publication Date (Print):October 1, 1984Publication History Published online1 May 2002Published inissue 1 October 1984https://pubs.acs.org/doi/10.1021/jo00194a055https://doi.org/10.1021/jo00194a055research-articleACS PublicationsRequest reuse permissionsArticle Views104Altmetric-Citations21LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Mutagenic and genotoxic effects of DNA adducts formed by the anticancer drugcis-diamminedichloroplatinum(II) (1995)

The toxicity and mutagenicity of three DNA adducts formed by the anticancer drug cis-diamminedichloroplatinum(II) (cis-DDP or cisplatin) were investigated in Escherichia coli. The adducts studied were cis-[Pt(NH3)2(d(GpG))] (G*G*), cis-[Pt(NH3)2(d(ApG))] (A*G*) and cis-[Pt(NH3)2(d(GpTpG))] (G*TG*), which collectively represent approximately 95% of the DNA adducts reported to form when the drug damages DNA. Oligonucleotide 24-mers containing each adduct were positioned at a known site within the viral strand of single stranded M13mp7L2 bacteriophage DNA. Following transfection into E. coli DL7 cells, the genomes containing the G*G*, A*G* and G*TG* adducts had survival levels of 5.2 +/- 1.2, 22 +/- 2.6 and 14 +/- 2.5% respectively, compared to unmodified genomes. Upon SOS induction, the survival of genomes containing the G*G* and A*G* adducts increased to 31 +/- 5.4 and 32 +/- 4.9% respectively. Survival of the genome containing the G*TG* adduct did not increase upon SOS induction. In SOS induced cells, the G*G* and A*G* adducts gave rise predominantly to G-->T and A-->T transversions respectively, targeted to the 5' modified base. In addition, A-->G transitions were detected for the A*G* adduct and low levels of tandem mutations at the 5' modified base as well as the adjacent 5' base were also observed for both adducts. The A*G* adduct was more mutagenic than the G*G* adduct, with a mutation frequency of 6% compared to 1.4% for the latter adduct. No cis-[Pt(NH3)2)2+ intrastrand crosslink-specific mutations were observed for the G*TG* adduct.
Proximity labeling of protein complexes and cell type-specific organellar proteomes in Arabidopsis enabled by TurboID (2019)

Abstract Defining specific protein interactions and spatially or temporally restricted local proteomes improves our understanding of all cellular processes, but obtaining such data is challenging, especially for rare proteins, cell types, or events. Proximity labeling enables discovery of protein neighborhoods defining functional complexes and/or organellar protein compositions. Recent technological improvements, namely two highly active biotin ligase variants (TurboID and miniTurboID), allowed us to address two challenging questions in plants: (1) what are in vivo partners of a low abundant key developmental transcription factor and (2) what is the nuclear proteome of a rare cell type? Proteins identified with FAMA-TurboID include known interactors of this stomatal transcription factor and novel proteins that could facilitate its activator and repressor functions. Directing TurboID to stomatal nuclei enabled purification of cell type- and subcellular compartment-specific proteins. Broad tests of TurboID and miniTurboID in Arabidopsis and N. benthamiana and versatile vectors enable customization by plant researchers.
Biodegradation of dimethylsilanediol in soils (1996)

The biodegradation potential of [14C]dimethylsilanediol, the monomer unit of polydimethylsiloxane, in soils was investigated. Dimethylsilanediol was found to be biodegraded in all of the tested soils, as monitored by the production of 14CO2. When 2-propanol was added to the soil as a carbon source in addition to [14C]dimethylsilanediol, the production of 14CO2 increased. A method for the selection of primary substrates that support cometabolic degradation of a target compound was developed. By this method, the activity observed in the soils was successfully transferred to liquid culture. A fungus, Fusarium oxysporum Schlechtendahl, and a bacterium, an Arthrobacter species, were isolated from two different soils, and both microorganisms were able to cometabolize [14C]dimethylsilanediol to 14CO2 in liquid culture. In addition, the Arthrobacter sp. that was isolated grew on dimethylsulfone, and we believe that this is the first reported instance of a microorganism using dimethylsulfone as its primary carbon source. Previous evidence has shown that polydimethylsiloxane is hydrolyzed in soil to the monomer, dimethylsilanediol. Now, biodegradation of dimethylsilanediol in soil has been demonstrated.
CDN Loop Detection (2019)

This document defines the CDN-Loop request header field for HTTP. CDN-
Loop addresses an operational need that occurs when an HTTP request is
intentionally forwarded between Content Delivery Networks (CDNs), but
is then accidentally or maliciously re-routed back into the original
CDN causing a non-terminating loop. The new header field can be used
to identify the error and terminate the loop.
Data from RhoB Determines Tumor Aggressiveness in a Murine EGFR<sup>L858R</sup>-Induced Adenocarcinoma Model and Is a Potential Prognostic Biomarker for Lepidic Lung Cancer (2023)

&lt;div&gt;Abstract&lt;p&gt;&lt;b&gt;Purpose:&lt;/b&gt; A crucial event in lung adenocarcinoma progression is the switch from an aerogenous spread toward an infiltrating tumor. Loss of RhoB expression has been suggested to be critical for lung cancer invasion. Here, we tested RhoB expression as a prognostic biomarker in non–small cell lung cancer (NSCLC) with a special focus on lepidic pattern.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Experimental Design:&lt;/b&gt; We analyzed RhoB expression using both IHC and RT-qPCR in two series of operated patients (&lt;i&gt;n&lt;/i&gt; = 100 and 48, respectively) and in a series of advanced lepidic adenocarcinoma (&lt;i&gt;n&lt;/i&gt; = 31) from different hospitals. Next, we examined the role of RhoB in lung cancer progression in transgenic mice that express inducible &lt;i&gt;EGFR&lt;sup&gt;L858R&lt;/sup&gt;&lt;/i&gt; crossed with &lt;i&gt;Rhob&lt;/i&gt; null mice.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Results:&lt;/b&gt; We identified that loss of RhoB expression was strongly associated with worse survival (&lt;i&gt;P&lt;/i&gt; = 0.0001) and progression-free survival (&lt;i&gt;P&lt;/i&gt; &lt; 0.001) in the first series. We then confirmed these results after multivariate analyses of the second series. In the series of adenocarcinoma with lepidic features issued from a clinical trial (IFCT-0401), we showed that loss of RhoB expression was associated with higher aggressiveness of stage IV. Finally, we showed that &lt;i&gt;EGFR&lt;sup&gt;L858R&lt;/sup&gt;&lt;/i&gt;/&lt;i&gt;Rhob&lt;/i&gt;&lt;sup&gt;+/+&lt;/sup&gt; mice developed mainly diffuse lung tumors with a lepidic pattern, whereas &lt;i&gt;EGFR&lt;sup&gt;L858R&lt;/sup&gt;&lt;/i&gt;/&lt;i&gt;Rhob&lt;/i&gt;&lt;sup&gt;+/−&lt;/sup&gt; and &lt;i&gt;EGFR&lt;sup&gt;L858R&lt;/sup&gt;&lt;/i&gt;/&lt;i&gt;Rhob&lt;/i&gt;&lt;sup&gt;−/−&lt;/sup&gt; developed a greater number of tumors, and aggressive adenocarcinomas with invasive properties.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Conclusions:&lt;/b&gt; We showed that RhoB is not only a strong prognostic factor in NSCLC but it is also critical for the acquisition of an aggressive phenotype of adenocarcinoma. &lt;i&gt;Clin Cancer Res; 20(24); 6541–50. ©2014 AACR&lt;/i&gt;.&lt;/p&gt;&lt;/div&gt;
[The immunodeficiency retrovirus in man and primates]. (1989)

Human immunodeficiency viruses, HIV-1 and HIV-2, are similar, by genome structure, nucleotide sequences and pathogenicity, to retroviruses existing in various primates, SIVs. The possibility is discussed that the human viruses have their origin in accidental transmission of some monkey viruses to man. Studies of SIVs also suggest that the pathogenic effect not only depends on the virus strain, but also on the host species.
Synthesis, Structure, and Tandem Mass Spectrometric Characterization of the Diastereomers of Quinic Acid (2016)

(-)-Quinic acid possess eight possible stereoisomers, which occur both naturally and as products of thermal food processing. In this contribution, we have selectively synthesized four isomers, namely, epi-quinic acid, muco-quinic acid, cis-quinic acid, and scyllo-quinic acid, to develop a tandem LC-MS method identifying all stereoisomeric quinic acids. Four derivatives have been unambiguously characterized by single-crystal X-ray crystallography. The missing diastereomers of quinic acid were obtained by nonselective isomerization of (-)-quinic acid using acetic acid/concentrated H2SO4 allowing chromatographic separation and assignment of all diastereomers of quinic acid. We report for the first time that a full set of stereoisomers are reliably distinguishable on the basis of their tandem mass spectrometric fragment spectra as well as their elution order. A rationale for characteristic fragmentation mechanisms is proposed. In this study, we also observed that muco-quinic acid, scyllo-quinic acid, and epi-quinic acid are present in hydrolyzed Guatemalan roasted coffee sample as possible products of roasting.
Reflection matrix approach for quantitative ultrasound imaging of scattering media (2020)

International audience
Clinical Experience With Transdermal Nicotine Patch in Tourette Syndrome (1999)

Abstract Stemming from the finding that nicotine potentiates haloperidol-induced catalepsy in rats, nicotine in the form of nicotine gum and as transdermal nicotine patch (TNP) has been used in open-label studies to obtund motor and vocal tics of children (age≥8 years, weighi≥25 kg), adolescents, and adults. Reduction of tics was seen during chewing of nicotine gum; the improvement lasted no longer than 1 hour after chewing. With a TNP in subjects who were not responding well to a variety of dopamine blockers, with some also receiving clonidine or a variety of selective serotonin reuptake inhibitors, motor and vocal tics were obtunded 45% over baseline in 85% of 35 subjects within 30 minutes to 3 hours after TNP application. Moreover, the relief of symptoms with a single 7-mg TNP, remaining on the skin for 24 hours, persisted for a variable period of time ranging from 1 to 120 days with an average of 10±2 days. Application of a second TNP for 24 hours when symptoms began to return resulted in a similar reduction in tic severity and frequency, which persisted an average of l3±3 days. Nicotine alone, without D 2 blockers, was successful in reducing premonitory urges to tic. After follow-up of 3 to 5 years, 19 of 35 patients continued to use the TNP in gradually decreasing frequency and with gradual reduction in dose of D 2 blockers. However, 16 patients (45%), as they grew into middle adolescence, discontinued use of the TNP, stating that they objected to the nausea induced by the patch. There was no evidence of habituation to nicotine. Side effects were not life threatenting; the most disturbing side effect was nausea, appearing 1 to 4 hours after the application of the patch and lasting 1 to 3 hours. There was no change in blood pressure; pulse rate increased from 5 to 10% within 3 hours but returned to baseline after 24 hours. Unsolved problems using TNP are mentioned and a putative mechanism for nicotine effectiveness in Tourette syndrome is briefly discussed.
Multiple Candidate Effectors from the Oomycete Pathogen Hyaloperonospora arabidopsidis Suppress Host Plant Immunity (2011)

Oomycete pathogens cause diverse plant diseases. To successfully colonize their hosts, they deliver a suite of effector proteins that can attenuate plant defenses. In the oomycete downy mildews, effectors carry a signal peptide and an RxLR motif. Hyaloperonospora arabidopsidis (Hpa) causes downy mildew on the model plant Arabidopsis thaliana (Arabidopsis). We investigated if candidate effectors predicted in the genome sequence of Hpa isolate Emoy2 (HaRxLs) were able to manipulate host defenses in different Arabidopsis accessions. We developed a rapid and sensitive screening method to test HaRxLs by delivering them via the bacterial type-three secretion system (TTSS) of Pseudomonas syringae pv tomato DC3000-LUX (Pst-LUX) and assessing changes in Pst-LUX growth in planta on 12 Arabidopsis accessions. The majority (~70%) of the 64 candidates tested positively contributed to Pst-LUX growth on more than one accession indicating that Hpa virulence likely involves multiple effectors with weak accession-specific effects. Further screening with a Pst mutant (ΔCEL) showed that HaRxLs that allow enhanced Pst-LUX growth usually suppress callose deposition, a hallmark of pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI). We found that HaRxLs are rarely strong avirulence determinants. Although some decreased Pst-LUX growth in particular accessions, none activated macroscopic cell death. Fewer HaRxLs conferred enhanced Pst growth on turnip, a non-host for Hpa, while several reduced it, consistent with the idea that turnip's non-host resistance against Hpa could involve a combination of recognized HaRxLs and ineffective HaRxLs. We verified our results by constitutively expressing in Arabidopsis a sub-set of HaRxLs. Several transgenic lines showed increased susceptibility to Hpa and attenuation of Arabidopsis PTI responses, confirming the HaRxLs' role in Hpa virulence. This study shows TTSS screening system provides a useful tool to test whether candidate effectors from eukaryotic pathogens can suppress/trigger plant defense mechanisms and to rank their effectiveness prior to subsequent mechanistic investigation.
Array-CGH Identifies Regions, Including the FOXP1 Locus, Associated with Different Clinical Outcome in Diffuse Large B-Cell Lymphomas (DLBCL) Treated with R-CHOP (2008)

Abstract Background. Diffuse large B-cell lymphoma (DLBCL) consists of a heterogeneous group of tumors. More than 30% of cases are not cured with R-CHOP chemotherapy. Aim. To identify genomic aberrations that could affect the response to therapy, we performed an arrayCGH study on uniformly treated DLBCL patients. Materials and Methods. Tumor samples were analyzed with Affymetrix Human Mapping 250K SNP arrays. Eligibility criteria were diagnosis of de novo DLBCL, first line treatment with R-CHOP or R-CHOP-like regimens, availability of frozen biopsy and of clinical baseline and follow-up data. Exclusion criteria were: primary mediastinal DLBCL, primary central nervous system DLBCL, HIV-positivity. Material has been collected according to the local IRB guidelines. Results. Genomic tumor profiles have been obtained in 163 samples from 10 Institutions; 23/163 cases were excluded from this analysis because the information on response was not available; 140/163 cases fulfilled the study requirements. The clinical parameters of the patients reflected the normal DLBLC population, as shown by the IPI score distribution (0–1 in 30%, 2 in 37%, 3 in 23% and 4–5 in 10%). The median follow-up was 23 months (range 1–1251). Complete remission was observed in 108/140 (77%) patients, partial response in 21 (15%), stable disease in 6 (4%) and progression in 5 (2%). Genomic differences were observed between complete responders (108 cases) and the remaining patients, grouped together as poor responders (32 cases). The latter group had more gains of 3p14.1 (FOXP1 locus), 3q29, 11q24.3, and losses of 2p11.2-p13.3, 8p23.1- pter, 10p12.31-p13, 15q11.2-q14, 15q21.1 and copy neutral LOH of chromosome 9p. On the converse, 1q gains were more common among patients achieving complete remission. No differences were observed for other common region of gains (7, 12, 18q/BCL2) or losses (1p, 6q, 17p/TP53). Conclusions. Specific genomic aberrations are associated with the response to R-CHOP in patients with DLBCL. In particular, the gain of the 3p14.1 (FOXP1 locus), which is associated with a lack of response to R-CHOP, suggests that the role of FOXP1 should be further investigated in DLBCL.
Supplementary Figures S1-S8 from Improved Treatment of Breast Cancer with Anti-HER2 Therapy Requires Interleukin-21 Signaling in CD8<sup>+</sup> T Cells (2023)

&lt;p&gt;IL-21R expression on splenocytes and TILs from H2N100 tumor-bearing mice (S1); ErbB-2 positive tumor cell lines do not express IL-21R (S2); CD8+ T cells and NK cells after ACT in Balb/c.Rag2-/- γc-/- mice (S3); B-cells and NKT cells are not likely to be required for anti-ErbB-2 mAb therapy (S4); Tfh cells are not likely to be required for anti-ErbB-2 mAb therapy (S5); Myeloid cell infiltrates in ErbB-2+ tumors (S6); Recombinant IL-21 improves therapeutic efficacy of anti-ErbB-2 mAb therapy (S7); Spearmans' correlations are shown between genes of interest, TILs as previously described and selected genes representing CD8+ T cell phenotype using patient's samples from the FinHER clinical trial (S8).&lt;/p&gt;
A New Soil Sampler and Elutriator for Collecting and Extracting Weed Seeds from Soil (1996)

A soil sampler, elutriator, and associated sample flushing device were designed and constructed for an intensive study of weed seedbanks. This equipment was used in 1993 to collect and process 4980 soil samples. The sampler was durable, core size was consistent, and sampling was efficient. Cores were approximately 200 cm 3 and two people could take 120 cores/h. The elutriator separated weed seeds from 36 of these cores at a time. Washing required 60 to 75 min depending on soil texture. Seeds as small as 0.3 mm in diam were recovered and almost 100% of the seeds were recovered from samples spiked with barnyardgrass, redroot pigweed, velvetleaf, and witchgrass. The flushing device was used to transfer sample contents from strainers of the elutriator to propyltex bags for drying and storing. Equipment like this, plus improved technology for identifying and counting seeds, is needed to make weed seedbank studies more feasible.
Towards fully integrated wideband transceivers: fundamental challenges, solutions and future (2005)

Design considerations of a generic wideband communication system differ significantly from its narrowband counterpart. In a wideband system, intermodulation distortion increases significantly, as the signal propagates through various active stages of RF/analog signal processing blocks. Thus, in contrary to the narrowband approach, it requires careful optimization of noise and linearity for a given power consumption. Many of these systems today utilize multicarrier modulation approach to provide robustness for dynamic wireless environment. Multicarrier modulation also aids the development of direct conversion transceivers leading to further optimization of signal processing algorithms at the baseband. In this paper, we would illustrate the architectural trade-off and design consideration of fully integrated direct conversion transceivers in standard silicon based processes. Fundamental considerations of power, linearity, effect of out-of-band blockers, coexistence with other standards would be addressed for a direct conversion UWB transceiver based on multi-band OFDM approach.
P2‐038: A highly sensitive diagnostic assay for early diagnosis of Alzheimer's disease (2009)

Today, Alzheimer's disease (AD) can be diagnosed with certainty only post mortem based on the presence of insoluble amyloid-β peptide (Aβ) aggregates and neurofibrillary tangles in the patient's brain tissue. Regarding the amyloid cascade hypothesis, Aβ oligomers and aggregates are directly involved in the pathogenic process. Therefore, presence of Aβ aggregates seem to be the most direct disease biomarker for AD and increasing effort is being made into the development of methods suitable for the detection of different Aβ aggregates in body fluids like CSF and blood. We developed a method which is able to count single Aβ aggregates from body fluids. The aggregates are captured to a surface, labelled by specific fluorescent probes and detected by scanning the surface with a confocal laser system (surface-FIDA). The assay was validated with synthetically prepared Aβ, showing linearity over a wide range of Aβ amounts in the ng up to pg range. First results show a good distinction between AD diseased people and non-demented controls by analysing cerebrospinal fluids (CSF) with surface-FIDA. Here we describe that surface FIDA is sensitive and specific enough to count single Aβ aggregates to be used as a minimally invasive tool for early diagnosis of AD, on line monitoring of disease progression and monitoring of therapeutic approaches.
ChemInform Abstract: Demonstration of Germancrene A as an Intermediate in 5‐Epi‐aristolochene Synthase Catalysis. (2000)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Slow-Light-Enhanced Silicon Optical Modulators Under Low-Drive-Voltage Operation (2012)

The integration of nanophotonics components with advanced complementary metal-oxide-semiconductor (CMOS) electronics requires drive voltages as low as 1 V for enabling next-generation CMOS electrophotonics transceivers. Slow-light propagation has been recently demonstrated as an effective mechanism to enhance the modulation efficiency in free-carrier-based electrooptical silicon modulators. Here, we exploit the use of slow light to reduce the driving voltage of carrier-depletion-based Mach-Zehnder modulators. The slow-light phase shifter consists of a p-n junction positioned in the middle of a corrugated waveguide. A modulation efficiency as high as <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">V</i> <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">π</sub> <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">L</i> <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">π</sub> ~ 0.6 V·cm is achieved, thus allowing data transmission rates up to 10 Gb/s with a 1.5-V <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">pp</sub> drive voltage and an insertion loss of ~12 dB. The influence of the drive voltage on the modulation speed as well as the variation of the insertion losses with a group index is also analyzed and discussed.