gebhart/patrepeval huggingface dataset:
A comparison of sample preparation methods for the study of the human serum proteome (2006)

This work describes our work of investigating the outcome of different sample preparation methods for label‐free quantitative proteomic study of human serum proteins. All analyses were preformed on insolution trypsin digestion of human serum. MS analysis was on a Q‐TOF instrument with a nanoflow split‐free LC system interfaced to the ion‐source (75 μ id LC columns). Data was acquired using data dependent analysis (DDA) or a high/low energy strategy. The high/low method is a “protein expression” method were a mass window of data is acquired in low energy (intact peptide) followed by a high energy acquisition on all ions in the mass window for intact peptides. This maximizes the duty cycle of the Q‐TOF to yield extensive qualitative and quantitative information by concurrently analyzing the peptide ions in the mixture. Samples were prepared by a verity of methods including, top‐6 or top‐20 abundant protein depletion and enhancement (reproducibility) of digestion with trypsin‐friendly surfactants. Fractionation of intact proteins by 1D SDS‐PAGE and chromatography was investigated. In addition, peptide mixtures from digestion were separated by multidimensional chromatography. The 2D methods included SCX/RP, RP/RP, and HILIC/RP. The objective was to understand the most effective sample preparation method for studying the human serum proteome. A summary of the results will be presented.
Inclusion of Cross-Linked Elastin in Gelatin/PEG Hydrogels Favourably Influences Fibroblast Phenotype (2020)

The capacity of a biomaterial to innately modulate cell behavior while meeting the mechanical property requirements of the implant is a much sought-after goal within bioengineering. Here we covalently incorporate soluble elastin into a gelatin-poly (ethylene glycol) (PEG) hydrogel for three-dimensional (3D) cell encapsulation to achieve these properties. The inclusion of elastin into a previously optimized gelatin-PEG hydrogel was then evaluated for effects on entrapped fibroblasts, with the aim to assess the hydrogel as an extracellular matrix (ECM)-mimicking 3D microenvironment for cellular guidance. Soluble elastin was incorporated both physically and covalently into novel gelatin/elastin hybrid PEG hydrogels with the aim to harness the cellular interactivity and mechanical tunability of both elastin and gelatin. This design allowed us to assess the benefits of elastin-containing hydrogels in guiding fibroblast activity for evaluation as a potential dermal replacement. It was found that a gelatin-PEG hydrogel with covalently conjugated elastin, supported neonatal fibroblast viability, promoted their proliferation from 7.3% to 13.5% and guided their behavior. The expression of collagen alpha-1(COL1A1) and elastin in gelatin/elastin hybrid gels increased 16-fold and 6-fold compared to control sample at day 9, respectively. Moreover, cells can be loaded into the hydrogel precursor solution, deposited, and the matrix cross-linked without affecting the incorporated cells adversely, thus enabling a potential injectable system for dermal wound healing.
Telavancin (Theravance). (2005)

Theravance is developing telavancin, an injectable peptidoglycan inhibitor antibiotic for potential use in the treatment of Gram-positive bacterial infection. Phase III trials in complicated skin and skin structure infections commenced in September 2004.
STUDY OF THE DIAMETER OF THE OBLIQUE CORRIDOR IN LATERAL DECUBITUS AND DORSAL DECUBITUS: RADIOLOGICAL STUDY (2022)

ABSTRACT Introduction: Degenerative intervertebral disc disease and its impact on quality of life when associated with sagittal misalignmentis a current topic in the literature. The technique known as OLIF derives from the need to use anterior cage stop romote stabilization of the affected segment, indirect decompression, restoration of segmental lordosis, and sagittal balance. Methods: Single-center, non-randomized, comparative, observational study. The following variables were measured using magnetic resonance imaging of the lumbar spine in dorsal and lateral decubitus, establishing a comparison between the size of the OLIF corridor in the L3L4 and L4L5 segments, as well as a comparison of corridor size between the different positions. Results: There was no difference incorridor size in the comparison between decubitus. However, when the L3L4 and L4L5 levels were compared, there was a significant difference in the size of the corridor in both the lateral and dorsal positions. Conclusion: The present study did not show any difference between the size of the OLIF corridor in L3L4 and L4L5 in the different decubitus, suggesting that thee valuation of the corridor in convention al magnetic resonance images appearstobe safe andreflects the actual size when positio ned for performing the OLIF technique. Level of evidence III; Retrospective study.
Application of 1.7-kV 700-A SiC LinPak to Optimize LCL Grid-Tied Converters (2019)

The current rating of SiC MOSFETs has been increased ever since its introduction. Yet, high current modules for 690-V and beyond grid applications, which would enable the realization of megawatt range converters, are not readily commercially available. Recently, a new high current 1.7-kV SiC MOSFET module suitable for hard-parallel connection to increase the current rating has been introduced. This paper investigates the system-level benefit of this module for a 2-level 690-V 1-MW grid-tied converter regarding achievable efficiency and power density and benchmarks the results against the achievable performance with conventional Si IGBTs. The study analyzes in detail the impact of the number of paralleled modules, the choice of the cooling system and the filter design.
Reel‐to‐Reel Continuous Chemical Solution Deposition of Epitaxial Gd 2 O 3 Buffer Layers on Biaxially Textured Metal Tapes for the Fabrication of YBa 2 Cu 3 O 7−δ Coated Conductors (2003)

A nonvacuum reel‐to‐reel dip‐coating system has been used to continuously fabricate epitaxial Gd 2 O 3 buffer layers on mechanically strengthened, biaxially textured Ni–(3 at.% W–1.7 at.% Fe), defined as Ni‐alloy, metal tapes. Because of its significance as a seed layer, optimum processing conditions (postannealing speed and temperature) of Gd 2 O 3 buffer layers have been studied. Highly textured films were obtained under reducing (96% Ar + 4% H 2 ) atmosphere at temperatures between 1100° and 1150°C; postannealing speed did not significantly affect the crystalline quality of the Gd 2 O 3 . Scanning electron microscopy revealed a continuous, dense, and crack‐free surface morphology for these dip‐coated buffers. The Gd 2 O 3 layer thickness led to pronounced differences in the growth characteristics of the subsequent YSZ and CeO 2 layers deposited by rf‐magnetron sputtering. Epitaxial YBCO films grown by pulsed laser deposition on the short prototype CeO 2 /YSZ/Gd 2 O 3 /Ni–(3 at.% W–1.7 at.% Fe) conductors yielded self‐field critical current densities ( J c ) as high as 1.2 × 10 6 A/cm 2 at 77 K. Pure Ni tapes were used to assess the viability of dip‐coated buffers for long length coated conductor fabrication. The YBCO films, grown on 80 cm long and 1 cm wide CeO 2 /YSZ/Gd 2 O 3 buffered Ni tapes by the industrially scalable ex situ BaF 2 precursor process, exhibited end‐to‐end self‐field J c values of 6.25 × 10 5 A/cm 2 at 77 K. These results demonstrate the reproducible epitaxy of solution‐derived seed layers on pure Ni and Ni‐alloy tapes as well as underscore the viability of solution approaches for the production of long length YBCO‐based coated conductors.
Directly modulated quantum dot lasers on silicon with a milliampere threshold and high temperature stability (2018)

Microring lasers feature ultralow thresholds and inherent wavelength-division multiplexing functionalities, offering an attractive approach to miniaturizing photonics in a compact area. Here, we present static and dynamic properties of microring quantum dot lasers grown directly on exact (001) GaP/Si. Effectively, a single-mode operation was observed at 1.3 μm with modes at spectrally distant locations. High temperature stability with T0∼103  K has been achieved with a low threshold of 3 mA for microrings with an outer ring radius of 15 μm and a ring waveguide width of 4 μm. Small signal modulation responses were measured for the first time for the microrings directly grown on silicon, and a 3 dB bandwidth of 6.5 GHz was achieved for a larger ring with an outer ring radius of 50 μm and a ring waveguide width of 4 μm. The directly modulated microring laser, monolithically integrated on a silicon substrate, can incur minimal real estate cost while offering full photonic functionality.
Turboprop+: Enhanced turboprop diffusion‐weighted imaging with a new phase correction (2012)

Faster periodically rotated overlapping parallel lines with enhanced reconstruction (PROPELLER) diffusion-weighted imaging acquisitions, such as Turboprop and X-prop, remain subject to phase errors inherent to a gradient echo readout, which ultimately limits the applied turbo factor (number of gradient echoes between each pair of radiofrequency refocusing pulses) and, thus, scan time reductions. This study introduces a new phase correction to Turboprop, called Turboprop+. This technique employs calibration blades, which generate 2-D phase error maps and are rotated in accordance with the data blades, to correct phase errors arising from off-resonance and system imperfections. The results demonstrate that with a small increase in scan time for collecting calibration blades, Turboprop+ had a superior immunity to the off-resonance-related artifacts when compared to standard Turboprop and recently proposed X-prop with the high turbo factor (turbo factor = 7). Thus, low specific absorption rate and short scan time can be achieved in Turboprop+ using a high turbo factor, whereas off-resonance related artifacts are minimized.
Rapid Progressing Allele HLA-B35 Px Restricted Anti-HIV-1 CD8+ T Cells Recognize Vestigial CTL Epitopes (2010)

This demonstrates a novel phenomenon that distinguishes individuals with the HLA-B*35-Px rapid progressing allele and those with the HLA-B*35-Py slower progressing allele.
Characterization and printability of Sodium alginate -Gelatin hydrogel for bioprinting NSCLC co-culture (2019)

3D bioprinting improves orientation of in vitro tumor models by offering layer by layer positioning of cancer cells and cancer associated fibroblasts (CAFs) which can replicate tumor microenvironment. Aim of this study was to develop a sodium alginate -gelatin (SA-GL) hydrogel by optimizing rheological parameters to print non-small cell lung cancer (NSCLC) patient derived xenograft (PDX) cells and lung CAFs co-cultures. SA-GL hydrogels were prepared, and rheological properties were evaluated. Both the cells were mixed with the hydrogel and printed using INKREDIBLE bioprinter. Hydrogels prepared with 3.25% and 3.5% (w/v) SA and 4% (w/v) GL showed higher printability and cell viability. A significant decline in viscosity with shear rate was observed in these hydrogels suggesting the shear thinning property of hydrogels. Spheroid size distribution after 15 days was in the diameter range of 50-1100 µm. Up-regulation of vimentin, α-SMA and loss of E-cadherin in co-culture spheroids confirmed cellular crosstalk. This study demonstrates that rheological optimization of SA-GL hydrogel enhances printability and viability of NSCLC PDX and CAF co-culture which allows 3D co-culture spheroid formation within the printed scaffold. Therefore, this model can be used for studying high throughput drug screening and other pre-clinical applications.
Effect of PEDOT Nanofibril Networks on the Conductivity, Flexibility, and Coatability of PEDOT:PSS Films (2014)

The use of poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) in electrodes and electrical circuits presents a number of challenges that are yet to be overcome, foremost amongst which are its relatively low conductivity, low coatability on hydrophobic substrates, and decreased conductivity at large strains. With this in mind, this study suggests a simple way to simultaneously address all of these issues through the addition of a small amount of a nonionic surfactant (Triton X-100) to commercial PEDOT:PSS solutions. This surfactant is shown to considerably reduce the surface tension of the PEDOT:PSS solution, thus permitting conformal coatings of PEDOT:PSS thin film on a diverse range of hydrophobic substrates. Furthermore, this surfactant induces the formation of PEDOT nanofibrils during coating, which led to the high conductivity values and mechanical stability at large strains (ε=10.3%). Taking advantage of the superior characteristics of these PEDOT:PSS thin films, a highly flexible polymer solar cell was fabricated. The power conversion efficiency of this solar cell (3.14% at zero strain) was preserved at large strains (ε=7.0%).
Friedel–Crafts sulfonylation of aromatics catalysed by solid acids: An eco-friendly route for sulfone synthesis (2000)

A mild and efficient catalytic method for Friedel–Crafts sulfonylation of arenes to the corresponding sulfones using a catalytic amount of reusable solid acid and arene- or alkanesulfonyl chlorides, sulfonic anhydrides and sulfonic acids as sulfonylating agents is described. Solid acids enable formation of a sulfone by the reaction of a sulfonic acid and an arene for the first time. In pursuit of the development of the best catalytic system, various metal-exchanged K10 montmorillonites and synthetic zeolites such as zeolite beta, zeolite Y and ZSM-5 have been screened for Friedel–Crafts sulfone synthesis. Fe3+-montmorillonite in the family of clays and zeolite beta in the family of zeolites exhibit higher activity. The activity is correlated to the presence of the right mix of Brønsted and Lewis acidic sites. The regioselective sulfonylation of toluene and naphthalene are studied in which para and beta selectivities are observed respectively.
Prospective evaluation of lymphoma response to immunomodulatory therapy criteria in GATA trial from the LYSA group (2023)

International audience
The Measurement and Prediction of Boundary Layer Entropy Generation Rate (2003)

In this paper hot wire measurements of the boundary layer velocity profiles at discrete locations along the suction surface of a turbine blade profile are presented. The measured aerodynamic data is transformed into thermodynamic data by way of the entropy function for the investigated Reynolds numbers of 76,000, 120,000 and 185,000 based on inlet velocity and chord length. The measurements are compared with predictions obtained from a commercially available computational fluid dynamics code and a prediction technique based on semi-empirical correlations. Interestingly the accuracy of the relatively simple semi-empirical technique, in respect to entropy production, is found to be comparable to that of the time consuming CFD code over the range of Reynolds numbers tested.
Rapidly dissociated autologous meniscus tissue enhances meniscus healing: Anin vitrostudy (2016)

Rapid dissociation of meniscus tissue may provide a single-step approach to augment regenerative healing of meniscus repairs.
ChemInform Abstract: NUCLEIC ACID RELATED COMPOUNDS PART 14, 3′‐O‐AMINOACYL‐2′‐O‐METHYLADENOSINES AND 2′‐O‐AMINOACYL‐3′‐O‐METHYLADENOSINES RELATED TO CHARGED TRNA TERMINI (1975)

Abstract Das Methyladenosin (I) reagiert mit den in situ aus den entsprechenden Boc‐Aminosäuren mit Dicyclohexylcarbodiimid gebildeten Aminosäureanhydriden (II) zu den Aminoacylderivaten (III).
Asymptotically Optimal Validated Asynchronous Byzantine Agreement (2019)

We provide a new protocol for Validated Asynchronous Byzantine Agreement in the authenticated setting. Validated (multi-valued) Asynchronous Byzantine Agreement is a key building block in constructing Atomic Broadcast and fault-tolerant state machine replication in the asynchronous setting. Our protocol has optimal resilience of ƒ < n/3 Byzantine failures and asymptotically optimal expected O(1) running time to reach agreement. Honest parties in our protocol send only an expected O(n2) messages where each message contains a value and a constant number of signatures. Hence our total expected communication is O(n2) words. The best previous result of Cachin et al. from 2001 solves Validated Byzantine Agreement with optimal resilience and O(1) expected time but with O(n3) expected word communication. Our work addresses an open question of Cachin et al. from 2001 and improves the expected word communication from O(n3) to asymptotically optimal O(n2).
Supplementary Tables S1 to S4 from Antitumor Activity of MEDI3726 (ADCT-401), a Pyrrolobenzodiazepine Antibody–Drug Conjugate Targeting PSMA, in Preclinical Models of Prostate Cancer (2023)

&lt;p&gt;Table S1. P values of comparing time to endpoint survival among all treatment groups in the LNCaP xenograft study Table S2. Summary of PSMA IHC in xenograft tumors Table S3. P values of comparing time to endpoint survival among all treatment groups in the CWR22Rv1 xenograft study Table S4. P values of comparing time to endpoint survival among all treatment groups in PC-3 xenograft study&lt;/p&gt;
Model of the kinetics of ketone bodies in humans (1982)

The kinetics of ketone bodies was studied in normal humans by giving a combined bolus intravenous injection of labeled acetoacetate ([14C]AcAc) and D(--)-beta-hydroxybutyrate (beta-[14C]-OHB) to seven subjects after an overnight fast, on two different occasions, and by collecting frequent blood samples for 100 min. Kinetic data were analyzed with both noncompartmental and compartmental modeling techniques. A four-compartment model, representing AcAc and beta-OHB in blood and two equilibrating ketone body compartments, inside the liver and extrahepatic tissues, was chosen as the most reliable mathematical representation; it is physiologically plausible and was able to accurately fit the data. The model permitted evaluation of the in vivo rate of ketone body production in the liver, the individual plasma clearance rates of AcAc and beta-OHB, their initial volumes of distribution, and the transfer rate parameters among the four ketone body compartments. Moreover, the model provided estimates of the components of the rates of appearance of AcAc and beta-OHB in plasma due to newly synthesized ketone body from acetyl-CoA in the liver, and to interconversion and recycling in the liver and extrahepatic tissues. The model also was used to evaluate other methodologies currently employed in the analysis of ketone body turnover data: the conventional approach based on use of the combined specific activity of AcAc and beta-OHB required assumptions not satisfied in vivo, leading to substantial errors in key parameter estimates.
Antibody of the IgG2 Subclass, Actinobacillus actinomycetemcomitans, and Early‐Onset Periodontitis (1996)

Susceptibility to early-onset periodontitis (EOP) appears to be attributable to a gene inherited in an autosomal dominant pattern. This explains why EOP clusters in families and why about half of the family members develop periodontal disease early in life. Manifestation of EOP is variable, with some patients having a localized form restricted to first molars and incisors (LJP) and others with a severe generalized form of periodontitis (SP). The extent and severity of disease is less in patients who are seropositive for Actinobacillus actinomycetemcomitans than in seronegative patients, and this relationship prompted the hypothesis that anti-A. actinomycetemcomitans helps limit disease. The dominant antibody is an IgG2 reactive with the serotypespecific carbohydrate. The incidence of the LJP form of EOP is about 10 times higher in blacks than in whites. Interestingly, blacks have higher levels of serum IgG2, a higher frequency of anti-A. actinomycetemcomitans antibody, and higher serum titers of IgG2 anti-A. actinomycetemcomitans which may help explain why the disease is localized. Studies in progress suggest that smoking reduces serum IgG2 levels in SP patients and is associated with more severe periodontal destruction. In marked contrast, IgG2 does not appear to be reduced in LJP patients who smoke, and smoking does not appear to increase periodontal destruction. We think that IgG2 anti-A. actinomycetemcomitans is playing a role in limiting the extent and severity of disease in patients genetically susceptible to EOP. J Periodontol 1996;67:317-322.
Multifunctional Nanoparticles for Targeted Chemophotothermal Treatment of Cancer Cells (2011)

Drug and heat delivery to tumors by active targeting with Herceptin-conjugated doxorubicin-loaded poly(ethylene glycol)–poly(lactic-co-glycolic acid)–Au half-shell nanoparticles (HER-DOX-PLGA-Au H-S NPs) is more effective than passive targeting with their HER-free counterparts (DOX-PLGA-Au H-S NPs). Thus, on NIR irradiation, higher intratumoral temperatures were reached in tumor-bearing mice that were treated with the HER-conjugated particles (see picture). Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Probabilities in safety of machinery—risk reduction through fixed and moveable guards by standardized impact tests, part 1: Applications and consideration of random effects (2017)

The uncontrolled ejection of a workpiece, a tool or parts of it can cause a perforation of the safety guards of machine tools with severe consequences for the operator. Therefore, experimental tests have been conducted in Germany and Italy during the last two decades in order to design specific safety guards with sufficient penetration resistance. As a result, a worldwide database has been established for metal- and woodworking machinery, and machine-specific design provisions have been collected in CEN and ISO product safety standards. Similar to all other experiments in the field of ballistic impacts, there are many parameters influencing the penetration behaviour: material of the target and its delivery condition, size, weight and shape, trajectory and impact angle of the projectile. In order to support the designer with practice-oriented provisions, different guard materials have been collected in design tables, in which the impact energy is allocated to the necessary thickness of the selected material. Formulas to estimate the worst-case projectile energy through technical machine specifications are also given. For the purpose of comparability, the details of the impact test procedures are also defined in the safety standards. This part 1 of the article describes how impact tests are being performed for different machine standards at IWF and INAIL laboratories. The assumptions and simplifications of the results are discussed on the background of the existing database. The deterministic interpretation narrows several significant influences on the results of the impact tests to a single test arrangement. It is not considered that the empirically determined penetration resistance, even in an accurate test mounting, has a statistical variation. Based on results of the IWF, it is shown that there is a considerable difference between a deterministic and a probabilistic interpretation. Consequently, a probabilistic review of existing design guidelines is given by focusing on a critical example with a reasonable statistical fundament: the perforation of a 12 mm thickness polycarbonate guard. As the backwards compatibility of existing design procedures is all important to manufacturers, at least the following two questions emerge: 1) How can the existing database be interpreted in a probabilistic way? 2) What is required to improve the current impact test and evaluation procedure to provide a more realistic picture of the risk of penetration? The commonly known "safety factor" seems to be the answer to ensure backwards compatibility with existing standards.