gebhart/patrepeval huggingface dataset:
PKC-θ is dispensable for OX40L-induced TCR-independent Treg proliferation (2017)

Abstract Previously, we have shown that GM-CSF-induced Bone marrow-derived dendritic cells (G-BMDCs), upon co-culture with splenic CD4+ T cells, have the capability to induce preferential proliferation of regulatory T cells (Tregs) in TCR-independent and OX40L/OX40 dependent manner. In this study we investigated the signaling involved in this Treg proliferation induced by G-BMDCs. Firstly, using splenic CD4+ T cells from OX40 deficient mice and G-BMDCs from WT mice, we confirmed the important role of OX40L/OX40 interaction in Treg expansion. Since OX40 stimulation in the absence of TCR engagement has been reported to induce the assembly of a signalosome involving PKC-θ, we investigated the requirement of PKC-θ in our Treg proliferation mechanism. Interestingly, PKC-θ deficient-CD4+ T cells showed impaired Treg proliferation upon co-culture with WT G-BMDCs. However, this defect in proliferation could be rescued upon IL-2 addition suggesting that PKC-θ was dispensable for Treg proliferation. We further demonstrated that PKC-θ was essential for T-effectors to provide IL-2, which was required for OX40L/OX40-induced Treg proliferation. Furthermore, injecting WT, OX40- deficient, and PKC-θ-deficient mice with soluble OX40L, we established that OX40L/OX40 signaling was sufficient to induce in vivo expansion of Tregs in WT and PKC- deficient mice, but not in OX40-deficient mice. Finally, our data suggested a possible involvement of TRAF1 in Tregs, downstream of OX40 activation.
Evaluated kinetic and photochemical data for atmospheric chemistry: Volume II – gas phase reactions of organic species (2006)

Abstract. This article, the second in the series, presents kinetic and photochemical data evaluated by the IUPAC Subcommittee on Gas Kinetic Data Evaluation for Atmospheric Chemistry. It covers the gas phase and photochemical reactions of Organic species, which were last published in 1999, and were updated on the IUPAC website in late 2002, and subsequently during the preparation of this article. The article consists of a summary table of the recommended rate coefficients, containing the recommended kinetic parameters for the evaluated reactions, and eight appendices containing the data sheets, which provide information upon which the recommendations are made.
Latency variable source separation for heart rate detection in low-quality ECG signals (2011)

Monitoring of the heart rate can provide vital clinical information, but can, in specific situations, be complicated due to the low signal to noise ratio (SNR) of the available physiological signals. Several methods to enhance the SNR are known from literature, e.g. wavelet-based enhancement methods, but most of these methods require a priori information on the recorded signals and are only applicable in specific situations. In this paper a generic method is presented that uses latency variable source separation (LVSS) to derive a matched filter for enhancement of electrocardiogram (ECG) signals. Besides its use on ECG signals, the LVSS method has the potential capability to enhance any kind of (quasi-) periodical signal. The LVSS method is evaluated by comparing its performance in SNR enhancement to the performance of a wavelet-based enhancement method. This performance demonstrates that for low-SNR ECG signals, the LVSS method outperforms the wavelet-based method.
Structural macrocyclic supramolecular chemistry (2014)

A graphical abstract is available for this content
A Highly Active and Coke‐Resistant Steam Reforming Catalyst Comprising Uniform Nickel–Iron Alloy Nanoparticles (2012)

Doing fine with Ni-Fe: The calcination and reduction of a hydrotalcite precursor containing Ni and Fe ions gives uniform Ni-Fe alloy nanoparticles mixed with Mg(Ni, Fe, Al)O particles. The uniformity of the Ni-Fe alloy nanoparticles is connected to the catalyst's high activity and resistance to coke formation in toluene and phenol steam reforming reactions.
Fast Computation of Many-Particle Hydrodynamic and Electrostatic Interactions in a Confined Geometry (2007)

An O(N) method is presented for calculation of hydrodynamic or electrostatic interactions between N point particles in a confined geometry. This approach splits point forces or sources into a local contribution for which rapidly decaying free-space analytical solutions to the Stokes or Poisson equations are used, and a global contribution whose effect is determined numerically using a fast iterative method. The scheme is applied to Brownian dynamics simulations of flowing confined polymer solutions, and the effects of concentration on hydrodynamically induced migration phenomena are illustrated.
Multimedia Capture, Collaboration and Knowledge Management (2011)

This chapter presents methods and technologies from Siemens Corporate Research that can assist in the process of creating multimedia collaborative knowledge bases: capture, querying, visualization, archiving, and reusability of multimedia knowledge bases. A selection of Siemens products in the healthcare and communication domains are introduced, above which novel multimedia collaboration and knowledge management technologies have been developed by the authors. With examples, it is explained how in concert these technologies can contribute to streamlining the processes within healthcare enterprises, telemedicine environments and home healthcare practices.
Student Branch Chapters and social networking [Chapter News] (2009)

When people talk about which universities have the strongest microwave program, will they mention your school? The typical route that students get involved with IEEE activities is by joining an IEEE Student Branch through their university; there are over 1,400 Student Branches worldwide. IEEE Student Members can then join MTT-S to receive Society periodicals and get discounts at MTT-S conferences.
Nervous System (2011)

This chapter contains sections titled: Nicotinic Acetylcholine Receptor Agonists: Target and Selectivity Aspects Chemical Structural Features of Commercialized Neonicotinoids Sulfoxaflor Spinosad and Spinetoram, a New Semi-Synthetic Spinosyn Sodium Channel-Blocking Insecticides Ligand-Gated Chloride Channel Antagonists (Fiproles) Chloride Channel Activators/New Natural Products: Avermectins and Milbemycins References
Methods for controlling pore morphology in aerogels using electric fields and products thereof (2023)

In one embodiment, an aerogel or xerogel includes column structures of a material having minor pores therein and major pores devoid of the material positioned between the column structures, where longitudinal axes of the major pores are substantially parallel to one another. In another embodiment, a method includes heating a sol including aerogel or xerogel precursor materials to cause gelation thereof to form an aerogel or xerogel and exposing the heated sol to an electric field, wherein the electric field causes orientation of a microstructure of the sol during gelation, which is retained by the aerogel or xerogel. In one approach, an aerogel has elongated pores extending between a material arranged in column structures having structural characteristics of being formed from a sol exposed to an electric field that causes orientation of a microstructure of the sol during gelation which is retained by the elongated pores of the aerogel.
Influence of the Irradiation Temperature on the Dosimetric and High-temperature TL peaks of Al2O3:C (2002)

The TL glow curves of Al2O3:C crystals have been investigated as a function of the irradiation temperature. The nature of the observed TL peaks has been studied by optical annealing. The filling of traps was found strongly dependent on the irradiation temperature in the case of UV exposure, which has been explained by the temperature dependence of the photoionisation of F centres. This latter phenomenon could have a part in the luminescence quenching and UV bleaching of F centres.
Cooperative Effect of Electron Spin Polarization in Chiral Molecules Studied with Non-Spin-Polarized Scanning Tunneling Microscopy (2022)

Polyalanine molecules (PA) with an α-helix conformation have recently attracted a great deal of interest, as the propagation of electrons through the chiral backbone structure comes along with spin polarization of the transmitted electrons. By means of scanning tunneling microscopy and spectroscopy under ambient conditions, PA molecules adsorbed on surfaces of epitaxial magnetic Al<sub>2</sub>O<sub>3</sub>/Pt/Au/Co/Au nanostructures with perpendicular anisotropy were studied. Thereby, a correlation between the PA molecules ordering at the surface with the electron tunneling across this hybrid system as a function of the substrate magnetization orientation as well as the coverage density and helicity of the PA molecules was observed. The highest spin polarization values, <i>P</i>, were found for well-ordered self-assembled monolayers and with a defined chemical coupling of the molecules to the magnetic substrate surface, showing that the current-induced spin selectivity is a cooperative effect. Thereby, <i>P</i> deduced from the electron transmission along unoccupied molecular orbitals of the chiral molecules is larger as compared to values derived from the occupied molecular orbitals. Apparently, the larger orbital overlap results in a higher electron mobility, yielding a higher <i>P</i> value. By switching the magnetization direction of the Co layer, it was demonstrated that the non-spin-polarized STM can be used to study chiral molecules with a submolecular resolution, to detect properties of buried magnetic layers and to detect the spin polarization of the molecules from the change in the magnetoresistance of such hybrid structures.
The improvement of polyethylene glycol-induced cell fusion by incubation of cells in a medium with increased NaCl concentration. (1988)

Incubation of cells in a medium with NaCl concentration increased by 0.2 mol/l for one hour prior to and one hour after the polyethylene glycol induced cell fusion improves the performance of fusion. This procedure increases the percentage of fused cells, the formation of massive fusion products (in case of fusion performed in suspension) and allows the improvement of cell viability. The possible mechanisms involved in such effects of increased NaCl concentration are discussed.
Metal organic frameworks enhanced dispersive solid phase microextraction of malathion before detection by UHPLC‐MS/MS (2020)

Metal organic frameworks are considered as an efficient and promised adsorbent for separation of several ions and compounds from solutions due to its unique geometric structure. Herein, copper-benzyl tricarboxylic acid based metal organic frameworks have showed a high efficiency in enrichment and microextraction of malathion from food and water samples. The microextraction procedures were followed by determination of malathion by ultra high performance liquid chromatography with tandem mass spectrometry. The optimum recoveries for malathion were obtained at pH 6, and with using 2 mL of ethyl acetate as the eluent. The microextraction procedures showed a detection limits and the quantification limits of 4.0 and 10.0 µg/L, respectively. The intra- and interday precision showed a relative standard deviation% less than 10. The feasibility of the proposed procedure was determined by evaluating the addition/recovery studies of malathion from the real samples. The absolute recovery% was ≥92%. Furthermore, some ions were tested as cointerfering ions, and the recovery% was 93-100%. These results confirm that the developed microextraction procedure based on copper-benzyl tricarboxylic acid based metal organic frameworks as extractor for dispersive solid phase microextraction is matrix-independent, and can be applied for various real samples including different matrix or various malathion content.
Multiparametric Sensing of Outer Membrane Vesicle-Derived Supported Lipid Bilayers Demonstrates the Specificity of Bacteriophage Interactions (2023)

The use of bacteriophages, viruses that specifically infect bacteria, as antibiotics has become an area of great interest in recent years as the effectiveness of conventional antibiotics recedes. The detection of phage interactions with specific bacteria in a rapid and quantitative way is key for identifying phages of interest for novel antimicrobials. Outer membrane vesicles (OMVs) derived from Gram-negative bacteria can be used to make supported lipid bilayers (SLBs) and therefore <i>in vitro</i> membrane models that contain naturally occurring components of the bacterial outer membrane. In this study, we employed <i>Escherichia coli</i> OMV derived SLBs and use both fluorescent imaging and mechanical sensing techniques to show their interactions with T4 phage. We also integrate these bilayers with microelectrode arrays (MEAs) functionalized with the conducting polymer PEDOT:PSS and show that the pore forming interactions of the phages with the SLBs can be monitored using electrical impedance spectroscopy. To highlight our ability to detect specific phage interactions, we also generate SLBs using OMVs derived from <i>Citrobacter rodentium</i>, which is resistant to T4 phage infection, and identify their lack of interaction with the phage. The work presented here shows how interactions occurring between the phages and these complex SLB systems can be monitored using a range of experimental techniques. We believe this approach can be used to identify phages that work against bacterial strains of interest, as well as more generally to monitor any pore forming structure (such as defensins) interacting with bacterial outer membranes, and thus aid in the development of next generation antimicrobials.
Abstract C23: Functional characterization of viable circulating tumor cells using nanowells. (2013)

Abstract Current characterizations of circulating tumor cells (CTCs) are limited to enumeration, genotyping, and gene expression. Because these assays each require fixation or lysis, it has not been possible to evaluate several important characteristics of live CTCs at the single-cell level. We have interrogated viable, single CTCs using arrays of subnanoliter wells (nanowells), performing functional measurements of viability, migration, and secretion of soluble factors. We developed a process using nanowells to isolate and characterize viable CTCs from whole blood. Each poly(dimethylsiloxane) (PDMS) array comprises 84,762 cubic wells of 275 pL each. CTCs were enriched from whole blood by negative selection against CD45 and loaded onto the array to settle into the wells by limiting serial dilution. Because CTCs are rare, the loading biased the occupancy of the wells to single CTCs or CTCs in preformed clusters, allowing comparisons among individual CTCs. The defined address of each well allowed spatiotemporal tracking of single CTCs, and subsequent registration of data for functional measures on each CTC. Using this approach, we made three types of measurements on viable CTCs isolated from prostate cancer patients: (1) immediate and short-term viability of CTCs, (2) migration and invasion potential of CTCs, and (3) secretion of soluble factors. We found a rare subset of CTCs to possess malignant traits indicative of metastatic potential in late-stage, progressing prostate cancer patients. These CTCs were resistant to anoikis after being in the circulation as they stained Calcein AM+/Annexin V−, and remained viable for at least a week in culture. They were invasive in their epithelial state when embedded in Matrigel. They could also secrete proteases capable of cleaving peptide substrates. However, we did not observe such metastatic potential in every CTC, pointing to the presence of heterogeneity within CTCs and suggesting that enumeration of CTCs alone may be insufficient to understand metastasis or stratify patients. By interrogating the functional behavior of individual CTCs, we identified a rare subset of CTCs with phenotypes consistent with more efficient metastasis. The nanowell technology holds the potential to further investigate the cellular characteristics of rare tumor cells and design therapies to counter metastasis. Citation Information: Mol Cancer Ther 2013;12(11 Suppl):C23. Citation Format: Xiaosai Yao, Atish D. Choudhury, Yvonne J. Yamanaka, William C. Hahn, K Dane Wittrup, J Christopher Love. Functional characterization of viable circulating tumor cells using nanowells. [abstract]. In: Proceedings of the AACR-NCI-EORTC International Conference: Molecular Targets and Cancer Therapeutics; 2013 Oct 19-23; Boston, MA. Philadelphia (PA): AACR; Mol Cancer Ther 2013;12(11 Suppl):Abstract nr C23.
ChemInform Abstract: Synthetic Studies Towards Keramamide F. (1995)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Collaborative interaction with volumetric displays (2008)

Volumetric displays possess a number of unique properties which potentially make them particularly suitable for collaborative 3D applications. Because such displays have only recently become available, interaction techniques for collaborative usage have yet to be explored. In this paper, we initiate this exploration. We present a prototype collaborative 3D model viewing application, which served as a platform for our explorations. We outline three design goals, discuss the key interaction issues which were encountered, and describe a suite of new techniques in detail. In initial user observation sessions, we found that our techniques allowed users to successfully complete a variety of 3D tasks. Furthermore, interviews with experts in potential usage domains indicated that the techniques we developed can serve as a baseline for future collaborative applications for volumetric displays.
Experimental Measurements on a Vortex Tube Separator Array (2022)

View Video Presentation: https://doi.org/10.2514/6.2022-3260.vid Vortex tube separators (VTSs) are used to filter harmful particles from helicopter engine inlets. They operate by turning the intake flow with helical vanes to create a vortical airstream, after which inertial particles are pushed to the outer peripheries by centrifugal force. An annular exit then separates the cleaner central flow from the particle-laden periphery flow. While patents and scientific publications have addressed the design of individual VTSs, in practice numerous separators are grouped together on a single VTS array with a common manifold – and very little attention has been given to these in the literature. The current work is an experimental investigation on VTS arrays, seeking to both establish a method for experimentation and study the flow emerging from an array’s clean air outlets. A VTS array model was fabricated and installed in a wind tunnel that was reconfigured for VTS flows, and particle image velocimetry (PIV) was conducted on a normal-to-flow measurement plane 7.62 cm downstream of the outlets. PIV results indicate axisymmetric flow with an edge-clinging vortex downstream of the VTS array model with seven separators, revealing significant impact by the duct wall. All but one separator was then blocked, resulting in a highly irregular flowfield with multiple recirculation zones, which suggested the presence of vortex breakdown phenomena upstream of the measurement plane. The possibility of vortex breakdown within the separators has potentially significant implications for VTS filtration; separation efficiency is highly sensitive to vortex characteristics and could be affected by breakdown characteristics such as unsteady back-pressure.
ChemInform Abstract: Enantioselective Synthesis of Boron‐Substituted Quaternary Carbon Stereogenic Centers Through NHC‐Catalyzed Conjugate Additions of (Pinacolato)boron Units to Enones. (2014)

Abstract The enantioselective boryl conjugate addition of bis(pinacolato)diboron to cyclic and linear α,β‐unsaturated ketones leads to corresponding boron‐substituted chiral ketones (III) and (V).
Identification of the ATP + Mg-dependent and polycation-stimulated protein phosphatases in the germinal vesicle of the Xenopus oocyte (1989)

Two protein phosphatase activities were characterized in the germinal vesicle of Xenopus laevis oocytes after manual dissection of the nucleus. One enzyme can be classified as an active form of the ATP + Mg-dependent (AMD) phosphatase, the other as a polycation-stimulated (PCS) phosphatase. The activity of the PCS phosphatase is localized exclusively in the soluble compartment of the nucleus (nucleoplasm). The catalytic subunit of the AMD phosphatase activity is associated either with the nuclear particulate fraction or with an inhibitory subunit in the nucleoplasm.
UNUSUAL MAGNETIC PROPERTIES OF SOME SIX-COÖRDINATE COBALT(II) COMPLEXES1 —ELECTRONIC ISOMERS (1961)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTUNUSUAL MAGNETIC PROPERTIES OF SOME SIX-COÖRDINATE COBALT(II) COMPLEXES1 —ELECTRONIC ISOMERSR. Carl Stoufer, Daryle H. Busch, and Wayne B. HadleyCite this: J. Am. Chem. Soc. 1961, 83, 17, 3732–3734Publication Date (Print):September 1, 1961Publication History Published online1 May 2002Published inissue 1 September 1961https://pubs.acs.org/doi/10.1021/ja01478a051https://doi.org/10.1021/ja01478a051research-articleACS PublicationsRequest reuse permissionsArticle Views782Altmetric-Citations61LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Electric Field Tuning of the Stop Band in a Liquid-Crystal-Infiltrated Polymer Inverse Opal (2002)

A polymer inverse opal with a tunable bandgap is presented. It is shown that, when the opal is infiltrated with a nematic liquid crystal, tuning of the stop band upon the application of an electric field is possible (see Figure for an SEM image of the structure). A discontinuous transition of the λLC shift upon a change in the voltage is observed.
History, Use, and Future of Microbial Insecticides (1993)

ALTHOUGH MICROBIAL INSECTICIDES account for <1% of the total insecticide market, microbial control of insect pests is gaining in importance. This is because of the improved performance and cost competitiveness of microbials in addition to increasing arthropod resistance to chemical insecticides, environmental concerns, and lack of development of new insecticidal chemistries. The use of microbial insecticides is growing at a rapid pace of 10–25% per year compared with chemical insecticides at 1–2% per year. Bacillus thuringiensis Berliner is primarily responsible for this growth. Microbial pest control agents including plants genetically engineered to produce pesticidal chemicals represent the largest number of recent registrations obtained by industry. Currently there are 20 microbial pesticides registered with the Environmental Protection Agency (EPA) (Table 1). Since 1984, EPA has reviewed 44 applications for small-scale field testing of genetically modified microbial pesticides. Since 1986, EPA has reviewed 40 applications for small-scale field testing of transgenic pesticidal plants.