gebhart/patrepeval huggingface dataset:
Chemical synthesis of novel taurine-containing uridine derivatives (2002)

Recently, novel taurine-containing uridine derivatives were discovered in mammalian mitochondrial tRNAs, and these modified ribonucleosides existed at the first position of the anti-codon. This paper describes the chemical synthesis of these novel uridine derivatives, 5-taurinomethyluridine (tau m5U) and 5-taurinomethyl-2-thiouridine (tau m5s2U). These taurine-containing uridine derivatives were synthesized in the good yields by the reaction of the corresponding 5-hydroxymethyluridine derivatives with taurine under basic conditions.
A conceptual framework for the development of iridium(iii) complex-based electrogenerated chemiluminescence labels (2019)

Translation of the highly promising electrogenerated chemiluminescence (ECL) properties of Ir(iii) complexes (with tri-<i>n</i>-propylamine (TPrA) as a co-reactant) into a new generation of ECL labels for ligand binding assays necessitates the introduction of functionality suitable for bioconjugation. Modification of the ligands, however, can affect not only the photophysical and electrochemical properties of the complex, but also the reaction pathways available to generate light. Through a combined theoretical and experimental study, we reveal the limitations of conventional approaches to the design of electrochemiluminophores and introduce a new class of ECL label, [Ir(C^N)<sub>2</sub>(pt-TOxT-Sq)]<sup>+</sup> (where C^N is a range of possible cyclometalating ligands, and pt-TOxT-Sq is a pyridyltriazole ligand with trioxatridecane chain and squarate amide ethyl ester), which outperformed commercial Ir(iii) complex labels in two commonly used assay formats. Predicted limits on the redox potentials and emission wavelengths of Ir(iii) complexes capable of generating ECL <i>via</i> the dominant pathway applicable in microbead supported ECL assays were experimentally verified by measuring the ECL intensities of the parent luminophores at different applied potentials, and comparing the ECL responses for the corresponding labels under assay conditions. This study provides a framework to tailor ECL labels for specific assay conditions and a fundamental understanding of the ECL pathways that will underpin exploration of new luminophores and co-reactants.
[Primary obstructive megaureter in infants: medical or surgical treatment? Apropos of 24 cases]. (1988)

The authors analyse 24 cases of primary obstructive megaureter diagnosed before the age of 3 months. 18 were discovered by antenatal echography between 28 and 39 weeks, whereas 6 were detected by the occurrence of early urinary tract infection. Initial investigations included echography, intravenous urography and micturating cystouretrogram. Primary megaureter was bilateral in 14 cases, unilateral in 12. According to the urographic classification of Beurton, there were 12 ureters stage III, 12 stage II, 10 stage I. 15 patients were operated, 4 of them during the first months (2 reimplantations, 2 urinary diversions). 12 were submitted to later reimplantation at the mean age of 15 months, one had a non functioning kidney removed. 9 were non operated and treated conservatively by antibacterials and regular supervision. Results analysis evaluated according to radiological stage and treatment showed that in more cases, surgery should be differed initially. Complete clinical and radiological resolution is possible even in severe obstructions. Antibacterial treatment beginning at birth favorises regression of dilated ureter. Thus reimplantation should be deferred undercover of regular biological and radiological survey. The age of 12 to 18 months is the most technically favorable time to ureteric reimplantation.
31 PORCINE SKIN-DERIVED STEM CELLS MAY BE A SUPERIOR SOURCE OF DONOR NUCLEI FOR EFFICIENT GENETIC MODIFICATION OF CLONED EMBRYOS (2008)

Somatic cell nuclear transfer (SCNT) in pigs relies primarily on the utilization of fetal-derived fibroblast cells, and the resultant clones tend to exhibit a significant level of phenotypic instability, which may be due to epigenetic reprogramming and/or genomic damage in the donor cells. In addition to the compromised phenotypic stability, production of transgenic clones through SCNT is inefficient, because the restricted lifespan of somatic donor cells in culture can be limiting when the genetic modification requires selection. In contrast, stem cells proliferate rapidly and do not undergo senescence at a high rate, so the selection process can be extended. Since there is no report of an embryonic stem cell line derived in the pig that could contribute to the germ line, we decided to investigate the utility of porcine skin-derived stem cells (SSCs). Porcine SSCs were isolated from the skin on the back of day 35 to 50 Yorkshire fetuses. The SSCs were cultured continually in SSCs medium (DMEM/F12 containing B-27, 20 ng mL–1 of epidermal growth factor, and 40 ng mL–1 of basic fibroblast growth factor) at 37.8°C, 5% CO2, 95% air. The SSCs expressed the neural progenitor marker nestin, as well as genes that are critical for pluripotency, such as Oct4 and Stat3. The SSCs proliferated actively in vitro and retained a normal karyotype after long-term culture. Electron microscopy revealed 2 distinct cell types within the spheres; elongated cells at the sphere periphery had invaginated nuclear envelopes and prominent nucleoli, and these cells displayed few, but large elongated mitochondria with transversal cristae as well as large cisternae of rough endoplasmic reticulum. In contrast, the cells in the center of the spheres were predominantly round-shaped, with a large round nucleus or cuboidal. The SSCs can be genetically modified with long-term positive selection, and 50 µg mL–1 G418 appeared to be an appropriate dose of G418 for selection of the transfected SSCs. Finally, NT embryos reconstructed with SSCs showed high rates of pre- and post-implantation development.The cell number in the blastocyst stage embryos derived from cloning with the SSC was significantly higher than those of the blastocysts derived from IVF (28.5 ± 1.9, 16.8 ± 4.0, respectively, P &amp;lt; 0.05), although there was no significant difference in blastocyst formation rates between these groups (21 to 25%). Three of the animals became pregnant in 4 surrogate gilts which received cloned embryos and reached to term. Two healthy male cloned piglets and 1 healthy female cloned piglet are genetically identical to the SSCs. Funding for this study was provided by the National Institutes of Health.
Broadband enhanced graphene photodetector with fractal metasurface(Conference Presentation) (2016)

Graphene has been demonstrated to be a promising photo-detection material because of its ultra-broadband absorption, compatibility with CMOS technology, and dynamic tunability. There are multiple known photo-detection mechanisms in graphene, among which the photovoltaic effect has the fastest response time thus is the prioritized candidate for ultrafast photodetector. There have been numerous efforts to enhance the intrinsically low sensitivity in graphene photovoltaic detectors using metallic plasmonic structures, but such plasmonic enhancements are mostly narrowband and polarization dependent. In this work, we propose a gold Cayley-tree fractal metasurface design that has a multi-band resonance, to realize broadband and polarization-insensitive plasmonic enhancement in graphene photovoltaic detectors. When illuminated with visible light, the fractal metasurface exhibits multiple hotspots at the metal-graphene interface, where the electric field of the incident electromagnetic wave is enhanced and contributes to generating excess electron-hole pairs in graphene. The large metal-graphene interface length in the fractal metasurface also helps to harvest at a higher efficiency the electron-hole pairs by built-in electric field due to metal-graphene potential gradient. To demonstrate the concept, we carried out experiment using Ar-Kr CW laser, an optical chopper, and lock-in amplifier. We obtained experimentally an almost constant ten-fold enhancement of photocurrent generated on the fractal metasurface compared to that generated on the plain gold-graphene edge, at all tested wavelengths (488 nm, 514 nm, 568 nm, and 647 nm). We also observed an unchanged photoresponse with respect to incident light polarization angles, which is a result of the highly symmetric geometry of the fractal metasurface.
Projection flow imaging by bolus tracking using stimulated echoes (1989)

Previous investigators have employed the concept of bolus tracking using either spin echoes or gradient echoes. In this paper we introduce two methods of bolus tracking using planar- and volume-selective stimulated echoes. The planar method employs a selective 90 degrees rf pulse which tags all spins in a particular plane. At a time tau 1 later, a nonselective 90 degrees rf pulse is employed, followed after a time tau 2, by another nonselective rf pulse. Only spins which experience all three rf pulses form a stimulated echo at time tau 1 after the third rf pulse. A balanced pair of flow-compensated dephasing (crusher) gradients further ensures that the stimulated echo is due only to the effect of all three rf pulses while minimizing flow dephasing. The first part of this gradient pair is applied after the initial rf pulse in the first tau 1 period to dephase the tagged spins. The second part of this gradient pair is applied after the third rf pulse to rephase the spins. Since the plane of the excited slice is orthogonal to the readout direction, flowing spins are imaged in an angiographic manner as they move away from the excited slice. A modification to this basic sequence excites only a small volume. In this manner, the suppression of stationary spins is effected by volume-selective excitation. In both the planar- and the volume-selective techniques, the excited spins undergo T1 and T2 relaxation during the tau 1 period but only T1 relaxation in the tau 2 period. In blood, where T1 is much greater than T2, keeping tau 1 as short as possible minimizes signal loss due to T2 dephasing. These methods demonstrate increased sensitivity compared to similar bolus tracking methods using either spin echoes or gradient echoes.
Fullerene derivatives with oligoethylene–glycol side chains: an investigation on the origin of their outstanding transport properties (2021)

For many years, fullerene derivatives have been the main n-type material of organic electronics and optoelectronics. Recently, fullerene derivatives functionalized with ethylene glycol (EG) side chains have been showing important properties such as enhanced dielectric constants, facile doping and enhanced self-assembly capabilities. Here, we have prepared field-effect transistors using a series of these fullerene derivatives equipped with EG side chains of different lengths. Transport data show the beneficial effect of increasing the EG side chain. In order to understand the material properties, full structural determination of these fullerene derivatives has been achieved by coupling the X-ray data with molecular dynamics (MD) simulations. The increase in transport properties is paired with the formation of extended layered structures, efficient molecular packing and an increase in the crystallite alignment. The layer-like structure is composed of conducting layers, containing of closely packed C<sub>60</sub> balls approaching the inter-distance of 1 nm, that are separated by well-defined EG layers, where the EG chains are rather splayed with the chain direction almost perpendicular to the layer normal. Such a layered structure appears highly ordered and highly aligned with the C<sub>60</sub> planes oriented parallel to the substrate in the thin film configuration. The order inside the thin film increases with the EG chain length, allowing the systems to achieve mobilities as high as 0.053 cm<sup>2</sup> V<sup>-1</sup> s<sup>-1</sup>. Our work elucidates the structure of these interesting semiconducting organic molecules and shows that the synergistic use of X-ray structural analysis and MD simulations is a powerful tool to identify the structure of thin organic films for optoelectronic applications.
Connectorized integrated star couplers on silicon (1992)

A connectorized integrated <i>N</i> x <i>N</i> passive star coupler suitable for use in single-mode multichannel optical networks is reported. The excess loss for most of the channels of the coupler is within 1.5 dB, with a worst value of 3 dB, of the unconnectorized coupling values, which range from 14.5 to 17.5 dB. The optical fibers are attached to the 19 x 19 coupler by butt-coupling a linear array of single-mode fibers using silicon v-grooves. Alignment is rapidly achieved with a compact six-degrees-of-freedom piezo-controlled micromanipulator. Best values of less than 0.1 dB per fiber attachment, dependent on connector quality, were obtained. The coupler demonstrated negligible reflection effects when used as the central element in a 1-Gb/s frequency-division-multiplex network experiment.
Id1 is a common downstream target of oncogenic tyrosine kinases in leukemic cells (2008)

Oncogenic tyrosine kinases, such as BCR-ABL, TEL-ABL, TEL-PDGFbetaR, and FLT3-ITD, play a major role in the development of hematopoietic malignancy. They activate many of the same signal transduction pathways. To identify the critical target genes required for transformation in hematopoietic cells, we used a comparative gene expression strategy in which selective small molecules were applied to 32Dcl3 cells that had been transformed to factor-independent growth by these respective oncogenic alleles. We identified inhibitor of DNA binding 1 (Id1), a gene involved in development, cell cycle, and tumorigenesis, as a common target of these oncogenic kinases. These findings were prospectively confirmed in cell lines and primary bone marrow cells engineered to express the respective tyrosine kinase alleles and were also confirmed in vivo in murine models of disease. Moreover, human AML cell lines Molm-14 and K562, which express the FLT3-ITD and BCR-ABL tyrosine kinases, respectively, showed high levels of Id1 expression. Antisense and siRNA based knockdown of Id1-inhibited growth of these cells associated with increased p27(Kip1) expression and increased sensitivity to Trail-induced apoptosis. These findings indicate that Id1 is an important target of constitutively activated tyrosine kinases and may be a therapeutic target for leukemias associated with oncogenic tyrosine kinases.
Cathodic Delamination (2022)

A process called “cathodic debonding” (a.k.a. “cathodic delamination”) is responsible for a significant portion of the adhesion-related failures in marine hardware. The process generates hydroxyl anions via the reduction of dissolved oxygen in seawater and it is often, but not exclusively, driven by the cathodic protection systems used to protect metal vessel hulls and fixed structures from anodic corrosion. The very high local pH conditions that develop on a cathodically polarized surface submerged in seawater are capable of damaging or destroying metal-polymer bonds, such as the one between an outboard cable's connector backshell and its protective polymer encapsulant, or between paints/primers and metallic surfaces. A variety of different approaches have been used to minimize the hardware loss and damage caused by cathodic debonding. Lowering the applied cathodic current and voltage will slow the process. The manipulation of certain environmental conditions, such as reducing the dissolved oxygen content of seawater, changing the concentration and kinds of cations present in the surrounding electrolyte, flowing versus stagnant water, and changes in temperature all will affect the rate of the cathodic delamination reaction. The use of coatings that are resistant to degradation under high pH conditions is also an option. The most commonly used coatings are known as “nonconducting coatings” or “NCCs.” Because they do not conduct electrical currents, NCCs do not support the cathodic delamination reaction on their surfaces, and since they are largely unaffected by high pH conditions, they tend to remain bonded to cathodically polarized metal surfaces when other coatings would have delaminated or physically deteriorated. The proper selection of materials and the careful manipulation of environmental conditions will minimize the instances of premature metal-polymer bond failures caused by cathodic debonding, and thereby help ensure that critical hardware remains functional for the longest possible amount of time in the marine environment.
The Utilization of Gas Chromatography/Mass Spectrometry in the Profiling of Several Antioxidants in Botanicals (2014)

The Utilization of Gas Chromatography/Mass Spectrometry in the Profiling of Several Antioxidants in Botanicals
Design of a SiGe Reconfigurable Power Amplifier for RF Applications: Device and Multi-standard Considerations (2006)

A low cost integrated reconfigurable power amplifier (PA), dedicated to multi-mode, multi-standard radio frequency front-end (RFFE), is proposed. Power considerations are taken into account in the design through the use of emitter ballasted HBT's and special care on the layout. The reconfigurable amplifier topology is presented, made up of two-stages independently controllable by biasing scheme. It allows the dynamic modification of the RF transistor quiescent current, to adapt both its linearity and its output power in order to fulfill several standards specifications. The PA uses 2.5 V supply voltage and was designed using a 0.25μm SiGe BiCMOS technology.
Wireless device monitoring methods, wireless device monitoring systems, and articles of manufacture (2023)

Wireless device monitoring methods, wireless device monitoring systems, and articles of manufacture are described. According to one embodiment, a wireless device monitoring method includes accessing device configuration information of a wireless device present at a secure area, wherein the device configuration information comprises information regarding a configuration of the wireless device, accessing stored information corresponding to the wireless device, wherein the stored information comprises information regarding the configuration of the wireless device, comparing the device configuration information with the stored information, and indicating the wireless device as one of authorized and unauthorized for presence at the secure area using the comparing.
Supplementary Data Table S3 from Selective Vulnerability to Pyrimidine Starvation in Hematologic Malignancies Revealed by AG-636, a Novel Clinical-Stage Inhibitor of Dihydroorotate Dehydrogenase (2023)

&lt;p&gt;GO analysis of proteins with at least a 1.5-fold increase in expression in the presence of AG-636 compared to DMSO&lt;/p&gt;
Refractive waveguide non-mechanical beam steering (NMBS) in the MWIR (2018)

Beam steering is a crucial technology for a number of applications, including chemical sensing/mapping and light detection and ranging (LIDAR). Traditional beam steering approaches rely on mechanical movement, such as the realignment of mirrors in gimbal mounts. The mechanical approach to steering has several drawbacks, including large size, weight and power usage (SWAP), and frequent mechanical failures. Recently, alternative non-mechanical approaches have been proposed and developed, but these technologies do not meet the demanding requirements for many beam steering applications. Here, we highlight the development efforts into a particular non-mechanical beam steering (NMBS) approach, refractive waveguides, for application in the MWIR. These waveguides are based on an Ulrich-coupled slab waveguide with a liquid crystal (LC) top cladding; by selectively applying an electric field across the liquid crystal through a prismatic electrode, steering is achieved by creating refraction at prismatic interfaces as light propagates through the device. For applications in the MWIR, we describe a versatile waveguide architecture based on chalcogenide glasses that have a wide range of refractive indices, transmission windows, and dispersion properties. We have further developed robust shadow-masking methods to taper the subcladding layers in the coupling region. We have demonstrated devices with &gt;10&deg; of steering in the MWIR and a number of advantageous properties for beam steering applications, including low-power operation, compact size, and fast point-to-point steering.
Gigapixel 1440-perspective display by sub-megapixel DMD (2021)

Desirable fields-of-view, angular resolutions, and form factors of near-To-eye AR/VR/MR displays require order-ofmagnitude increases in pixel count and pixel density of spatial light modulators (SLM). We present an in-plane angularspatial light modulation technique to increase the independent output display pixels of a DMD by three orders of magnitude to achieve gigapixel output from a sub-megapixel device. Pulsed illumination synchronized to a DMD's micromirror actuation realizes pixel-implemented and diffraction-based angular modulation, and fine source array control increases angular selectivity. The gigapixel output is demonstrated in a 1440-perspective display, each perspective having the DMD's full native XGA resolution, across a 43.9°×1.8° FOV viewing angle. 8-bit multi-perspective videos at 30 FPS are demonstrated, and pixel-implemented multi-focal-plane image generation is realized. Implications for near-To-eye displays are discussed. © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.
Computing Ion Solvation Free Energies Using the Dipolar Poisson Model (2009)

A new continuum model is presented for computing the solvation free energies of cations in water. It combines in a single formalism based on statistical thermodynamics the Poisson model for electrostatics with the Langevin dipole model to account for nonuniform water dipole distribution around the ions. An excellent match between experimental and computed solvation free energies is obtained for 10 monovalent and divalent ions.
Study of polymer molecules and conformations with a nanopore (2023)

The invention features methods for evaluating the conformation of a polymer, for example, for determining the conformational distribution of a plurality of polymers and to detect binding or denaturation events. The methods employ a nanopore which the polymer, e.g., a nucleic acid, traverses. As the polymer traverses the nanopore, measurements of transport properties of the nanopore yield data on the conformation of the polymer.
Extending Transactional Memory with Atomic Deferral (2018)

This paper introduces atomic deferral, an extension to TM that allows programmers to move long-running or irrevocable operations out of a transaction while maintaining serializability: the transaction and its de- ferred operation appear to execute atomically from the perspective of other transactions. Thus, program- mers can adapt lock-based programs to exploit TM with relatively little effort and without sacrificing scalability by atomically deferring the problematic operations. We demonstrate this with several use cases for atomic deferral, as well as an in-depth analysis of its use on the PARSEC dedup benchmark, where we show that atomic deferral enables TM to be competitive with well-designed lock-based code.