gebhart/patrepeval huggingface dataset:
Interferometric, Hybrid, and Weighted $$$B_1^+$$$ Mapping for Expedited RF Calibration of Parallel Transmit Ultrahigh-Field MRI (2023)

With an increasing number of transmit channels, faster methods for acquiring $$$B_1^+$$$ maps on pTx MRI systems grow in importance. We compared different interferometry modes for accurate absolute $$$B_1^+$$$ mapping using satTFL. We also introduced weighted hybrid $$$B_1^+$$$ mapping as an attempt to improve hybrid $$$B_1^+$$$ mapping. In this, a second absolute $$$B_1^+$$$ map was introduced as an attempt to further shorten measurement duration while keeping acceptable accuracy. From the tested interferometry modes, phase-cycling performed best in both absolute and weighted mapping. Weighted hybrid mapping exceeded conventional hybrid mapping substantially and could therefore be sufficiently accurate for many applications.
Genome-wide transcriptomic analysis of the Lactobacillus plantarum WCFS1 response to resveratrol (2017)

Póster presentado a la VII International Conference on Environmental Industrial and Applied Microbiology, celebrada en Madrid (España) del 18 al 20 de octubre de 2017.
Hammerhead Ribozyme-Mediated Cleavage of the Human Insulin-Like Growth Factor-II Ribonucleic Acid in Vitro and in Prostate Cancer Cells* (1999)

Insulin-like growth factor (IGF)-II plays an important role in fetal growth and development. IGFs are potent mitogens for a variety of cancer cells. A paracrine/autocrine role of IGF-II in the growth of breast and prostate cancer cells has been suggested. To test the role of IGF-II in cancer cell growth, hammerhead ribozymes targeted to human IGF-II RNA were constructed. Single (R)- and double (RR)-ribozymes were catalytically active in vitro whereas mutant ribozymes (M or MM) did not cleave IGF-II RNA. RR was more active than R. In human prostate cancer PC-3 cells, both R and RR similarly suppressed IGF-II messenger RNA (mRNA) levels (approximately 40%) compared with the level in parental or M-expressing PC-3 cells. Polymerase II and III promoter-driven R similarly suppressed IGF-II mRNA levels. Suppression of IGF-II mRNA levels by R was associated with suppression of IGF-II protein levels. R- (or RR-) expressing PC-3 cells did not grow under serum-starved conditions and showed prolonged doubling times in the presence of 10% FCS compared with those of parental or M-expressing cells. These results substantiated that IGF-II plays a critical role in prostate cancer cell growth.
Correction - Mechanism of Inactivation of γ-Aminobutyric Acid -α-Ketoglutaric Acid Aminotransferase by 4-Amino-5-halopentanoic Acids (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCorrection - Mechanism of Inactivation of γ-Aminobutyric Acid -α-Ketoglutaric Acid Aminotransferase by 4-Amino-5-halopentanoic AcidsRichard Silverman and Mark LevyCite this: Biochemistry 1981, 20, 17, 5094Publication Date (Print):August 18, 1981Publication History Published online13 November 2003Published inissue 18 August 1981https://pubs.acs.org/doi/10.1021/bi00520a607https://doi.org/10.1021/bi00520a607research-articleACS PublicationsRequest reuse permissionsArticle Views20Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
NCKAP1Ldefects lead to a novel syndrome combining immunodeficiency, lymphoproliferation, and hyperinflammation (2020)

The Nck-associated protein 1-like (NCKAP1L) gene, alternatively called hematopoietic protein 1 (HEM-1), encodes a hematopoietic lineage-specific regulator of the actin cytoskeleton. Nckap1l-deficient mice have anomalies in lymphocyte development, phagocytosis, and neutrophil migration. Here we report, for the first time, NCKAP1L deficiency cases in humans. In two unrelated patients of Middle Eastern origin, recessive mutations in NCKAP1L abolishing protein expression led to immunodeficiency, lymphoproliferation, and hyperinflammation with features of hemophagocytic lymphohistiocytosis. Immunophenotyping showed an inverted CD4/CD8 ratio with a major shift of both CD4+ and CD8+ cells toward memory compartments, in line with combined RNA-seq/proteomics analyses revealing a T cell exhaustion signature. Consistent with the core function of NCKAP1L in the reorganization of the actin cytoskeleton, patients' T cells displayed impaired early activation, immune synapse morphology, and leading edge formation. Moreover, knockdown of nckap1l in zebrafish led to defects in neutrophil migration. Hence, NCKAP1L mutations lead to broad immune dysregulation in humans, which could be classified within actinopathies.
ChemInform Abstract: SYNTHESIS OF N,N‐DIARYLTOLUENE‐4‐SULFONAMIDES (1976)

Abstract Durch Erhitzen der sek. Sulfonamide (I) mit den Brombenzolen (II) in Gegenwart von Cu‐Bronze und wasserfreiem K‐carbonat entstehen die tert. Sulfonamide (III) in unterschiedlichen Ausbeuten.
Failure of ES‐62 to inhibit T‐helper type 1 responses to other filarial nematode antigens (2008)

ES-62 is a secreted protein of filarial nematodes that possesses multiple immunomodulatory activities. A full characterization of these activities awaits elucidation but to date it has been shown that ES-62 can inhibit pro-inflammatory/Th1 immune responses and in some studies, it has been found to actively support Th2 development. As an active filarial nematode infection is associated with a Th2-like immunological phenotype, this study investigated whether ES-62 was likely to be responsible for, or at least contribute to, this phenotype. Specifically, we determined ES-62's effect on the immune response to two other filarial nematode antigens, chosen for their ability to promote Th1 responses. The two antigens were recombinant Onchocerca volvulus-Fatty acid And Retinol-binding-1 (rOv-FAR-1) and recombinant Onchocerca volvulus-Activation associated Secreted Protein-1 (Ov-ASP-1). Overall the results show that in spite of its previously characterized immunomodulatory properties, ES-62 was unable to modulate/reverse the Th1 immune responses induced by the two Onchocerca antigens. Therefore, in this study no support is provided for the idea that ES-62 might be a major player in facilitating the overall immunological phenotype in filariasis and reasons for this somewhat surprising outcome are discussed.
A CELL CULTURE VACCINE AGAINST BOVINE EPHEMERAL FEVER (1978)

A vaccine was prepared from cell culture fluids harvested from the twelfth passage of the 919 strain of bovine ephemeral fever (BEF) virus in Vero cell cultures. Cattle were vaccinated subcutaneously with various combinations of strain 919 virus and adjuvants. Neutralising antibodies were assayed at various times after vaccination and some cattle were challenged by intravenous inoculation with the virulent 417WBC strain of BEF virus. Strain 919 virus of the third and twelfth passage levels in Vero cells produced neither fever, clinical illness nor detectable viraemia in 5 calves inoculated intravenously. Nor could viraemia be detected in 5 heifers receiving vaccine subcutaneously. When the vaccine was administered mixed with aluminium hydroxide adjuvant, the production of neutralising antibodies increased with an increase in the volume of vaccine from 2.5 ml to 10 ml and the response to 2 injections was significantly better than the response to a single injection. The neutralising antibody response was decreased when vaccine was diluted in phosphate buffered saline. The neutralising antibody response following 2 subcutaneous vaccinations with strain 919 virus mixed with aluminium hydroxide adjuvant was higher than that following intravenous inoculation with virulent virus. The vaccine-induced antibodies persisted for at least 12 months, and revaccination at this time led to an increase in the titre of neutralising antibody. Antibodies induced by a single subcutaneous administration of strain 919 virus mixed with Freund's complete adjuvant persisted for at least 40 weeks; those induced by vaccine containing Freund's incomplete adjuvant had virtually disappeared within 16 weeks. All these calves responded to vaccination with aluminium hydroxide-containing vaccine with increases in levels of neutralising antibodies. Of 26 vaccinated calves challenged with virulent BEF virus, 24 remained clinically normal. Two developed brief periods of pyrexia on the seventh day after challenge, but no other clinical signs. One of these calves had a viraemia that was demonstrated only by intravenous inoculation of a susceptible calf. The remaining calf had no detectable viraemia. All of 7 unvaccinated calves developed severe clinical BEF within 5 days of challenge. No disease attributable to the 919 virus occurred in 24 vaccinated pregnant heifers or their newborn calves.
Post‐partum recovery course in patients with gestational hypertension and pre‐eclampsia (2014)

A post-partum observation period of 12 weeks is acceptable for differentiating PE and GH from chronic hypertension or renal disease. GH severity did not affect the recovery period, but proteinuria severity did. Onset time was a factor influencing the recovery from PE and GH.
Weighted Flowtime on Capacitated Machines (2013)

It is well-known that SRPT is optimal for minimizing flow time on machines that run one job at a time. However, running one job at a time is a big under-utilization for modern systems where sharing, simultaneous execution, and virtualization-enabled consolidation are a common trend to boost utilization. Such machines, used in modern large data centers and clouds, are powerful enough to run multiple jobs/VMs at a time subject to overall CPU, memory, network, and disk capacity constraints. Motivated by this prominent trend and need, in this work, we give the first scheduling algorithms to minimize weighted flow time on such capacitated machines. To capture the difficulty of the problem, we show that without resource augmentation, no online algorithm can achieve a bounded competitive ratio. We then investigate algorithms with a small resource augmentation in speed and/or capacity. Our first result is a simple (2 + ε)-capacity O(1/ε)-competitive greedy algorithm. Using only speed augmentation, we then obtain a 1.75-speed O(1)-competitive algorithm. Our main technical result is a near-optimal (1 + ε)-speed, (1+ε)-capacity O(1/ε3)-competitive algorithm using a novel combination of knapsacks, densities, job classification into categories, and potential function methods. We show that our results also extend to the multiple unrelated capacitated machines setting.
Structure and Function of CC-Chemokine Receptor 5 Homologues Derived from Representative Primate Species and Subspecies of the Taxonomic Suborders Prosimii and Anthropoidea (2003)

A chemokine receptor from the seven-transmembrane-domain G-protein-coupled receptor superfamily is an essential coreceptor for the cellular entry of human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV) strains. To investigate nonhuman primate CC-chemokine receptor 5 (CCR5) homologue structure and function, we amplified CCR5 DNA sequences from peripheral blood cells obtained from 24 representative species and subspecies of the primate suborders Prosimii (family Lemuridae) and Anthropoidea (families Cebidae, Callitrichidae, Cercopithecidae, Hylobatidae, and Pongidae) by PCR with primers flanking the coding region of the gene. Full-length CCR5 was inserted into pCDNA3.1, and multiple clones were sequenced to permit discrimination of both alleles. Compared to the human CCR5 sequence, the CCR5 sequences of the Lemuridae, Cebidae, and Cercopithecidae shared 87, 91 to 92, and 96 to 99% amino acid sequence homology, respectively. Amino acid substitutions tended to cluster in the amino and carboxy termini, the first transmembrane domain, and the second extracellular loop, with a pattern of species-specific changes that characterized CCR5 homologues from primates within a given family. At variance with humans, all primate species examined from the suborder Anthropoidea had amino acid substitutions at positions 13 (N to D) and 129 (V to I); the former change is critical for CD4-independent binding of SIV to CCR5. Within the Cebidae, Cercopithecidae, and Pongidae (including humans), CCR5 nucleotide similarities were 95.2 to 97.4, 98.0 to 99.5, and 98.3 to 99.3%, respectively. Despite this low genetic diversity, the phylogeny of the selected primate CCR5 homologue sequences agrees with present primate systematics, apart from some intermingling of species of the Cebidae and Cercopithecidae. Constructed HOS.CD4 cell lines expressing the entire CCR5 homologue protein from each of the Anthropoidea species and subspecies were tested for their ability to support HIV-1 and SIV entry and membrane fusion. Other than that of Cercopithecus pygerythrus, all CCR5 homologues tested were able to support both SIV and HIV-1 entry. Our results suggest that the shared structure and function of primate CCR5 homologue proteins would not impede the movement of primate immunodeficiency viruses between species.
HBM package integration: Technology trends, challenges and applications (2016)

Tb/s low latency bandwidth and lower system power is driving the need for HBM adoption. Silicon Interposer (2.5D) is the incumbent technology of choice. Potentially lower cost, fine pitch interconnect wafer-level and substrate based technologies are emerging. To drive broader adoption of HBM applications (cooling limited) and higher performance stacks (8-Hi), higher HBM junction temperature (>95C) needs to be supported.
Vector modulator for W-band software radar techniques (2001)

Direct-carrier modulation is an attractive technique for low-cost high-performance radar transceivers. In this paper, it is shown that, when the technique is applied to a generic homodyne radar architecture, the signaling waveform can be software adapted without requiring any hardware modifications. The key circuit in this novel software radar is a W-band monolithic I-Q vector modulator employing two push-pull (bi-phase) amplitude modulators. To fully exploit this circuit's capacity to generate accurate constellations at millimeter-wave frequencies, a generalized theoretical analysis of the I-Q (push-pull) vector modulator is presented. This is a comprehensive analysis of the topology and does not assume ideal components. As a demonstration of the vector modulator's flexibility, a 76.5-GHz MMIC version has been fabricated and characterized by means of static S-parameter measurements and by several modulation spectra. Based on the theoretical model and the measured results, the I-Q (push-pull) vector modulator promises to be a vital component for the realization of future software radar.
New Scale Inhibitor for Harsch Field Conditions (2000)

The aim of the work presented in this paper is to developp the best polymer usable for BaSO4 and CaCO3 scale inhibition in harsh field conditions (3 ≤ pH ≤ 6, 130 °C ≤ T°C ≤ 200°C, 2 ≤[Ca2+] ≤ 20 g/l, adsorption on the reservoir to allow injection by squeeze). The first objective was the thermal stability. It is known that sulfonated group permits to obtain it. Thus homopolymers made with different sulfonated monomers have been aged three week at 200°C and it has been checked by Infra Red measurements that their structure were not damaged by the test. Because in some cases after degradation of the polymer, the obtained oligomers can have scale inhibition behaviour but not adsorption. Then the monomers leading to the more stable polymers have been copolymerized with monomers bearing carboxylate function and their thermal stability have been checked. The second objective was the scale inhibition and the third was the neutral behaviour in contact with high levels of Ca2+. Series of copolymers have been synthesized in order to define what compositions and molecular weights were convenient to obtain the simultaneous inhibition of BaSO4 and CaCO3 scale and no precipitation when in contact with high levels of calcium ions. The last objective but not the least is the adsorption of the copolymer on the rock. This property is necessary for using the anti-scale additive in squeeze. For the polymers which answered at the first three conditions, injections in rock sample have been made to check the adsorption ability.
Verification (2004)

Aristotle maintained that women have fewer teeth than men; although he was twice married, it never occurred to him to verify this statement by examining his wives' mouths.Bertrand Russell, (1872–1970).
ChemInform Abstract: Five New Peltogynoids from Underground Parts of Iris bungei: A Mongolian Medicinal Plant. (2002)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
COPII vesicles can affect the activity of antisense oligonucleotides by facilitating the release of oligonucleotides from endocytic pathways (2018)

RNase H1-dependent, phosphorothioate-modified antisense oligonucleotides (PS-ASOs) can enter cells through endocytic pathways and need to be released from the membrane-enclosed organelles, a limiting step for antisense activity. Accumulating evidence has suggested that productive PS-ASO release mainly occurs from late endosomes (LEs). However, how PS-ASOs escape from LEs is not well understood. Here, we report that upon PS-ASO incubation, COPII vesicles, normally involved in ER-Golgi transport, can re-locate to PS-ASO-containing LEs. Reduction of COPII coat proteins significantly decreased PS-ASO activity, without affecting the levels of PS-ASO uptake and early-to-late endosome transport, but caused slower PS-ASO release from LEs. COPII co-localization with PS-ASOs at LEs does not require de novo assembly of COPII at ER. Interestingly, reduction of STX5 and P115, proteins involved in tethering and fusion of COPII vesicles with Golgi membranes, impaired COPII re-localization to LEs and decreased PS-ASO activity. STX5 can re-locate to LEs upon PS-ASO incubation, can bind PS-ASOs, and the binding appears to be required for this pathway. Our study reveals a novel release pathway in which PS-ASO incubation causes LE re-localization of STX5, which mediates the recruitment of COPII vesicles to LEs to facilitate endosomal PS-ASO release, and identifies another key PS-ASO binding protein.
Intermolecular Alignment in β2-Microglobulin Amyloid Fibrils (2010)

The deposition of amyloid-like fibrils, composed primarily of the 99-residue protein β2-microglobulin (β2m), is one of the characteristic symptoms of dialysis-related amyloidosis. Fibrils formed in vitro at low pH and low salt concentration share many properties with the disease related fibrils and have been extensively studied by a number of biochemical and biophysical methods. These fibrils contain a significant β-sheet core and have a complex cryoEM electron density profile. Here, we investigate the intrasheet arrangement of the fibrils by means of (15)N-(13)C MAS NMR correlation spectroscopy. We utilize a fibril sample grown from a 50:50 mixture of (15)N,(12)C- and (14)N,(13)C-labeled β2m monomers, the latter prepared using 2-(13)C glycerol as the carbon source. Together with the use of ZF-TEDOR mixing, this sample allowed us to observe intermolecular (15)N-(13)C backbone-to-backbone contacts with excellent resolution and good sensitivity. The results are consistent with a parallel, in-register arrangement of the protein subunits in the fibrils and suggest that a significant structural reorganization occurs from the native to the fibril state.
The most suitable size design of the ball capacitor (2005)

This paper introduces the design method of the most suitable size of the homocentric ball capacitor on the condition that the maximum pressure endurance is limited and the puncture field is strong,and gives the most suitable size of the concentric ball capacitor.
Weighted Population Code for low power neuromorphic image classification (2016)

Recent digital spiking neuromorphic chips can perform complex computations in real-time with very low power consumption. The input data to such systems needs to first be converted into spikes using a spike encoding scheme. Current examples of such schemes include rate codes and population codes. The selected coding scheme might heavily impact the system's energy consumption, communication bandwidth, processing frame-rate, and computation accuracy. Hence it is important to make an educated decision when selecting the most appropriate spike coding scheme for a given task. To this end, we present a novel spike coding scheme named Weighted Population Code (WPC). WPC is compared to existing coding schemes to transduce images for classification using the TrueNorth chip. Extensive on-chip experimentation with the MNIST and the Flickr-LOGOS32 datasets sheds light on the trade-offs between accuracy, bandwidth, frame rate, network size and energy consumption for image classification, showing the advantages of WPC when high dynamic range and accuracy are needed.
Table S2 from Integrative Kinome Profiling Identifies mTORC1/2 Inhibition as Treatment Strategy in Ovarian Clear Cell Carcinoma (2023)

&lt;p&gt;Supplementary Table 2 | Gene set for Haloplex sequencing.&lt;/p&gt;