gebhart/patrepeval huggingface dataset:
Stability and proteolysis of immunotoxins in patient urine after treatment (2007)

B97 BL22 and LMB-2 are Immunotoxins (ITs) composed of the Fv portion of an antibody (scFv) of anti-CD25 and anti-CD22 respectively and a truncated fragment (38kDa) of Pseudomonas exotoxin A (PE38), which inactivates Elongation Factor 2. Patients with leukemia and lymphoma have been treated with these ITs with major responses in CLL and complete remissions in hairy cell leukemia. The half-life of these ITs in blood is about 2-3 hours. However, there is little known about the excretion of ITs into urine by humans. We know that in mice, 6 h after an injected dose of LMB2, one-third of the injected dose is excreted in urine, and over two-thirds after 24 h. Studying the renal excretion of ITs in humans may be critical to understanding hemolytic uremic syndrome, a limiting toxicity of BL22 but not of LMB-2.  To evaluate the pattern of urinary excretion of immunotoxins in humans, we evaluated the rate of IT excretion in patient urine and the catabolism occurring in the urine. We recovered the total protein from urine by TCA precipitation or purified the ITs from urine using Q-Sepharose anion exchange chromatography. We detected the presence of ITs by Western Blot Assay. We studied the stability of BL22 added to urine at different time points as a function of pH. Finally, we quantified the amount of ITs in patient urine using ELISA assay. The ELISA assay was able to test the presence of ITs in patient’s urine or ITs exogenously added to healthy donor urine. The accuracy of the ELISA assay was >80% in the absence of urine, but accuracy decreases to
Germany-wide DWDM field trial: Transparent connection of a long haul link and a multiclient metro network (2001)

We report on error-free multiclient transmission over a Germany-wide all-optical, configurable DWDM system. The performance of the transparent connection of a metropolitan area network and a bi-directional long haul system is investigated under real-world conditions.
Architectural Enhancements in Intel® Agilex™ FPGAs (2020)

This paper describes architectural enhancements in Intel® Agilex™ FPGAs and SoCs. Agilex devices are built on Intel's 10nm process and feature next-generation programmable fabric, tightly coupled with a quad-core ARM processor subsystem, a secure device manager, IO and memory interfaces, and multiple companion transceiver tile choices. The Agilex fabric features multiple logic block enhancements that significantly improve propagation delays and integrate more effectively with the second-generation HyperFlexAgilex™ pipelined routing architecture. Routing connections are re-designed to be point-to-point, dropping intermediate connections featured in prior FPGA generations and replacing them with a wider variety of shorter wire types. Fine-grain programmable clock skew and time-borrowing were introduced throughout the fabric to augment the slack-balancing capabilities of HyperFlex registers. DSP capabilities are also extended to natively support new INT9/BFLOAT16/FP16 formats. Together, along with process and circuit enhancements, these changes support more than 40% performance improvement over the Stratix® 10 family of FPGAs.
Investigation and modulation of interleukin-6 following subarachnoid hemorrhage: targeting inflammatory activation for cerebral vasospasm (2022)

Correlation between IL-6 and CV has been well documented. We show that a mechanistic connection exists via the p-STAT3 pathway, and IL-6 blockade provides benefit in reducing CV and its consequences mediated by myeloid cell origin diapedesis.
2P004 Vibrational Spectroscopic Implication of the Role of R14 Residue in Human Hematopoietic Prostaglandin D_2 Synthase(29. Protein structure and dynamics (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006) (2006)

In studies invelving thc E,coli chaperonin GroEL, little analyses has beell performed regarding the relationshtp between the connectivity of the amino-acid sequence and chaperonin function. In this study, we evaluated this relatienship by constructing a circulurly
Mucus Glycoprotein Structure, Gel Formation and Gastrointestinal Mucus Function (1984)

Gastrointestinal mucus occurs as a water-insoluble gel adherent to the mucosal surfaces and as a viscous, mobile solution in the lumen. The adherent gastroduodenal mucus gel is part of the mucosal defence against acid (with HCO3-), pepsin (diffusion barrier) and mechanical damage. Rheological studies show that gastrointestinal mucus is a weak, viscoelastic gel. The size and physical properties of the isolated component glycoproteins depend critically on the methods used to obtain them. A glycoprotein preparation of Mr approximately 2 X 10(6), which possesses the gel-forming properties of the native mucus, is considered to represent the secreted covalent entity in pig gastric and small intestinal mucus. These glycoproteins have a polymeric structure of subunits joined by disulphide bridges between non-glycosylated regions of their protein cores. Glycoprotein polymerization, essential for gel formation, is deficient in gastric mucus in peptic ulcer disease. In vivo, adherent mucus gel forms a thin but continuous cover of variable thickness (rat 5-500 microns) over the gastroduodenal mucosa. Luminal pepsin rapidly dissolves this mucus cover and its continuity is maintained by fresh mucus secretion. Bile, HCl, 2 M-NaCl and ethanol (less than 40%) do not destroy mucus gel structure. Prostaglandins and carbachol increase mucus thickness, affording better protection, but it is thought that continuity of the protective mucus cover is the critical factor in its protective functions.
Formin isoforms are differentially expressed in the mouse embryo and are required for normal expression of gf-4 and shh in the limb bud (1995)

Mice homozygous for the recessive limb deformity (ld) mutation display both limb and renal defects. The limb defects, oligodactyly and syndactyly, have been traced to improper differentiation of the apical ectodermal ridge (AER) and shortening of the anteroposterior limb axis. The renal defects, usually aplasia, are thought to result from failure of ureteric bud outgrowth. Since the ld locus gives rise to multiple RNA isoforms encoding several different proteins (termed formins), we wished to understand their role in the formation of these organs. Therefore, we first examined the embryonic expression patterns of the four major ld mRNA isoforms. Isoforms I, II and III (all containing a basic amino terminus) are expressed in dorsal root ganglia, cranial ganglia and the developing kidney including the ureteric bud. Isoform IV (containing an acidic amino terminus) is expressed in the notochord, the somites, the apical ectodermal ridge (AER) of the limb bud and the developing kidney including the ureteric bud. Using a lacZ reporter assay in transgenic mice, we show that this differential expression of isoform IV results from distinct regulatory sequences upstream of its first exon. These expression patterns suggest that all four isoforms may be involved in ureteric bud outgrowth, while isoform IV may be involved in AER differentiation. To define further the developmental consequences of the ld limb defect, we analyzed the expression of a number of genes thought to play a role in limb development. Most significantly, we find that although the AERs of ld limb buds express several AER markers, they do not express detectable levels of fibroblast growth factor 4 (fgf-4), which has been proposed to be the AER signal to the mesoderm. Thus we conclude that one or more formins are necessary to initiate and/or maintain fgf-4 production in the distal limb. Since ld limbs form distal structures such as digits, we further conclude that while fgf-4 is capable of supporting distal limb outgrowth in manipulated limbs, it is not essential for distal outgrowth in normal limb development. In addition, ld limbs show a severe decrease in the expression of several mesodermal markers, including sonic hedgehog (shh), a marker for the polarizing region and Hoxd-12, a marker for posterior mesoderm. We propose that incomplete differentiation of the AER in ld limb buds leads to reduction of polarizing activity and defects along the anteroposterior axis.
Conducting On-orbit Gene Expression Analysis on ISS: WetLab-2 (2013)

WetLab-2 will enable expanded genomic research on orbit by developing tools that support in situ sample collection, processing, and analysis on ISS. This capability will reduce the time-to-results for investigators and define new pathways for discovery on the ISS National Lab. The primary objective is to develop a research platform on ISS that will facilitate real-time quantitative gene expression analysis of biological samples collected on orbit. WetLab-2 will be capable of processing multiple sample types ranging from microbial cultures to animal tissues dissected on orbit. WetLab-2 will significantly expand the analytical capabilities onboard ISS and enhance science return from ISS.
Multi-Carrier HSPA Evolution (2009)

The HSPA Evolution will during the next decade be a key radio access technology for a cost-effective provisioning of mobile broadband services. In addition to the continuous improvements of WCDMA/HSPA in terms of spectral efficiency and latency, "multi-carrier operation" (or "carrier aggregation") for individual connections is now introduced. This paper presents the evolution of Multi-Carrier HSPA, discusses implications on network architecture & user equipment, and evaluates the achievable system performance (with focus on downlink). It is concluded that Multi-Carrier HSPA can be implemented at a low incremental cost; both in the radio access network, which already supports multiple carriers, and in terminals (due to synergies with LTE). Numerical results further show that achievable data rates of a Multi-Carrier HSPA system increase proportional to the number of carriers; in terms of peak physical layer data rates as well as (for bursty packet data) average user throughput. Thus, Multi-Carrier HSPA will be an attractive means for operators to provide higher data rates and decrease the production cost of mobile broadband access.
Presence of luteinizing hormone‐releasing hormone fragments in the rhesus monkey forebrain (2001)

Previously, we have shown that two types of luteinizing hormone-releasing hormone (LHRH) -like neurons, "early" and "late" cells, were discernible in the forebrain of rhesus monkey fetuses by using antiserum GF-6, which cross-reacts with several forms of LHRH. The "late" cells that arose from the olfactory placode of monkey fetuses at embryonic days (E) 32-E36, are bona fide LHRH neurons. The "early" cells were found in the forebrain at E32-E34 and settled in the extrahypothalamic area. The molecular form of LHRH in "early" cells differs from "late" cells, because "early" cells were not immunopositive with any specific antisera against known forms of LHRH. In this study, we investigated the molecular form of LHRH in the "early" cells in the nasal regions and brains of 13 monkey fetuses at E35 to E78. In situ hybridization studies suggested that both "early" and "late" LHRH cells expressed mammalian LHRH mRNA. Furthermore, "early" cells predominantly contain LHRH1-5-like peptide and its cleavage enzyme, metalloendopeptidase E.C.3.4.24.15 (EP24.15), which cleaves LHRH at the Tyr5-Gly6 position. This conclusion was based on immunocytochemical labeling with various antisera, including those against LHRH1-5, LHRH4-10, or EP24.15, and on preabsorption tests. Therefore, in primates, a group of neurons containing mammalian LHRH mRNA arises at an early embryonic stage before the migration of bona fide LHRH neurons, and is ultimately distributed in the extrahypothalamic region. These extrahypothalamic neurons contain LHRH fragments, rather than fully mature mammalian LHRH. The origin and function of these neurons remain to be determined.
Quality factor of real beams: measurement problems and practical solutions (1993)

The recent introduction of the beam quality factor 1 can be thought of as a major forward step towards the assessment of the spatial quality of general optical beams with a sole numerical parameter.
Selective catalysis of A-T base pair proton exchange in DNA complexes: imino proton NMR analysis (1987)

Polyaromatic molecules with amino chain substituents, upon binding with DNA, selectively catalyze exchange of the A.T base pair protons with bulk water protons. The amine-catalyzed exchange is mediated by compounds which are A.T and G.C base sequence specific, intercalators, and outside binders. A mechanism for the selective exchange, involving transient opening and closing of individual A.T base pairs in the duplex, is discussed.
Desmopressin after cardiac surgery in bleeding patients. A multicenter randomized trial (2016)

This multicenter randomized trial demonstrated that, in patients pre-treated with tranexamic acid, desmopressin should not be expected to improve treatment of patients who experience bleeding after cardiac surgery.
A Semiempirical Scaling Model for the Solid- and Liquid-State Photopolymerization Kinetics of Semicrystalline Acrylated Oligomers (2018)

The recent introduction of semicrystalline acrylated oligomers exhibiting fast photoinitiated free radical polymerization in the solid state calls for a deeper understanding of the mechanisms behind the reaction kinetics. The photoinduced polymerization of an acrylated urethane-based poly(ethylene glycol) precursor was studied in detail at temperatures below the melting point using differential photocalorimetry. In isothermal conditions, the exothermal heat flow profile is characterized by an acceleration step followed by a gradual deceleration. In contrast to liquid-state photopolymerization, the well-known gel effect cannot be invoked to account for the reaction acceleration in the crystallized resin. By revisiting the kinetics of free-radical polymerization, it appears that the acceleration results from the buildup of the radical concentration toward steady state in a reaction–diffusion driven process. The kinetic behavior is examined in terms of conversion for which any structure-dependent kinetic effect is described by a power-law approximation based on scaling arguments from experimental evidence and polymer physics. This results in a closed-form analytical expression that compares well to experimental data for the photopolymerization kinetics of a semicrystalline acrylated urethane precursor upon adjustment of three parameters. The model is extended to include the additional kinetic complexity for liquid (meth)acrylates and provides a unified approach to free-radical polymerization built on fundamental insights.
Cell volume and rate of proliferation, but not protein expression pattern, distinguish pup/intimal smooth muscle cells from subcultured adult smooth muscle cells (2001)

Smooth muscle cells from neonatal rats and from injured blood vessels grow with a characteristic cobblestone morphology that distinguishes them from adult smooth muscle cells. This has led to the proposition that there are two distinct types of smooth muscle cells with different proliferative capacity. Here we systematically compare the properties of subcultured adult smooth muscle cells in culture and clonal lines of cobblestone smooth muscle cells from both neonatal rats and injured vessels. The cobblestone smooth muscle cells have a significantly smaller average cell volume, estimated using two different flow cytometry measurements. However, the two types of smooth muscle cells have indistinguishable protein expression patterns when the levels of more than 20 different proteins (including cytoskeletal proteins, matrix proteins, cytokines, cytokine receptors, adhesion molecules and enzymes) are measured by quantitative immunofluorescence. Furthermore, in contrast to previous observations, we demonstrate that both types of smooth muscle cells secrete a powerful mitogenic activity. The higher cell density achieved by the cobblestone smooth muscle cells in culture was responsible for the earlier reports that this mitogenic activity was secreted only by cobblestone smooth muscle cells. We conclude that many of the differences seen between cobblestone smooth muscle cells and adult smooth muscle cells in vitro (proliferation rate, morphology, protein expression pattern, secretion of mitogenic activity) could be attributable to a stable difference in the median cell volume of the cultures.
Expression of Proteasomal Immunosubunit ß1i Is Dysregulated in Inflammatory Infiltrates of Minor Salivary Glands in Sjögren’s Syndrome (2009)

The proteasomal ss1i subunit is dysregulated in peripheral white blood cells and in inflammatory infiltrates of minor salivary glands in patients with SS.
Water Quality and Heavy Metal Monitoring in Water, Sediments, and Tissues of the African Catfish Clarias gariepinus (Burchell, 1822) from the River Nile, Egypt (2010)

Water quality of the river Nile and trace elements of the water, sediments and fish tissues were investigated in the current work. Eighteen different sampling points were selected along the whole course of the River Nile from its spring at Aswan to its estuaries at Rosetta and Damietta. Higher mean value of conductivity, alkalinity, chemical oxygen demand (COD), total organic carbon (TOC), ammonia (NH3), nitrate (NO3), total solid (TS), sulphate (SO4), chloride (Cl), orthophosphate were recorded in the water of Damietta and Rosetta branches comparing to other sites. Also trace metals in the water, sediments and tissues of Clarias gariepinus increased significantly (P Fe > Cu > Pb > Mn > Cr> Cd > Hg. In the gill tissues theses metals were accumulated in the following order Fe > Zn > Mn > Pb > Cr > Cu > Cd> Hg. The low accumulation of metals in muscle may be due to lack of binding affinity of these metals with the proteins of muscle. This is particularly important because muscles contribute the greatest mass of the flesh that is consumed as food.
Abstract B089: The suppression of ERa activity promotes the mitochondrial deficient CD133hi/Notch3hi/ERalow cancer stem cell phenotype via autocrine IL6 in luminal breast cancer (2013)

Abstract The inhibition of ERa pathway (hormonal therapy -HT-) is the most effective therapeutic strategy for metastatic luminal breast cancer. However, the efficacy of HT is time-limited, suggesting that metastatic disease will eventually employ an ERa-independent biochemistry. Here we demonstrated that CD133hi/CD44low tumor initiating cells arise following ERa lack of function via oxidative phosphorylation (OXPHOS) dysfunction. These cells display a remarkable migrating and metastatic phenotype, which associate with the downregulation of ERa and the increased expression of Notch3. Chronic lack of ERa activity by tamoxifen generates CD133hi/Notch3hi/ERalow via the up-regulation of tumor-derived IL6. We then showed that IL6 confers resistance to ERa lack of function by up-regulating glycolysis/OXPHOS. Finally, higher expression of CD133, Notch3 and IL6 identifies ERalow luminal breast cancers endowed with metastatic and self-renewal potentials. In conclusion, our data demonstrate that oxidative phosphorylation dysfunction and autocrine IL6 are key molecular triggers of intra-tumor heterogeneity and tumor initiating capabilities in luminal breast cancer refractory to anti ERa target therapy. Citation Format: Pasquale Sansone, Massimiliano Bonafe, Claudio Ceccarelli, Marjan Berishaj, Jacqueline Bromberg. The suppression of ERa activity promotes the mitochondrial deficient CD133hi/Notch3hi/ERalow cancer stem cell phenotype via autocrine IL6 in luminal breast cancer. [abstract]. In: Proceedings of the AACR Special Conference on Advances in Breast Cancer Research: Genetics, Biology, and Clinical Applications; Oct 3-6, 2013; San Diego, CA. Philadelphia (PA): AACR; Mol Cancer Res 2013;11(10 Suppl):Abstract nr B089.
Power Law Liquid - A Unified Form of Low-Energy Nodal Electronic Interactions in Hole Doped Cuprate Superconductors (2015)

The strange-metal phase of the cuprate high temperature superconductors, above where the superconductivity sets in as a function of temperature, is widely considered more exotic and mysterious than the superconductivity itself. Here, based upon detailed angle resolved photoemission spectroscopy measurements of Bi$_2$Sr$_2$CaCu$_2$O$_8$$_+$$_δ$ over a wide range of doping levels, we present a new unifying phenomenology for the non-Fermi liquid normal-state interactions (scattering rates) in the nodal direction. This new phenomenology has a continuously varying power law exponent (hence named a Power Law Liquid or PLL), which with doping varies smoothly from a quadratic Fermi Liquid to a linear Marginal Fermi Liquid and beyond. Using the extracted PLL parameters we can calculate the optics and resistivity over a wide range of doping and normal-state temperature values, with the results closely matching the experimental curves. This agreement includes the presence of the $"$pseudogap$"$ temperature scale observed in the resistivity curves including the apparent quantum critical point. This breaks the direct link to the pseudogapping of antinodal spectral weight observed at similar (but here argued to be different) temperature scales, and also gives a new direction for searches of the microscopic mechanism at the heart of these and perhaps many other non-Fermi-liquid systems.
Design space exploration of high-speed busses using statistical methods (2003)

This paper presents an efficient statistical method to increase the data rate of local I/O bus. Parametric yield of the PCI-X has been computed at a higher data rate. Then yield loss at higher data rate has been recovered by making the most feasible and effective adjustments. Instead of full factorial signal integrity analysis, sensitivity relations and statistical distributions of signal integrity measures have been computed, which supply detailed information to designers and manufacturers.
Effect of Polarity of Small Molecule Interlayer Materials on the Open Circuit Voltage and Power Conversion Efficiency of Polymer Solar Cells (2011)

A series of small molecule based interlayer materials with different functional groups were synthesized and evaluated as cathode modifiers at the interface between the poly(3-hexylthiophene) (P3HT):[6,6]-phenyl C61-butyric acid methyl ester (PCBM) active layer and Al cathode. The device performances of the organic solar cells (OSCs) were correlated with the interface properties between the small molecule interface modifiers and the Al cathode. High open circuit voltage (Voc) was obtained in the OSC with highly polar phosphine oxide compound, while low Voc was observed in the OSC with less polar phosphine chalconide interlayer. The interface between the interlayer and Al was analyzed with ultraviolet photoelectron spectroscopy, and the energy level shift at the interface was correlated with the Voc and polarity of the interlayer. Highly polar phosphine oxide compound induced large energy level shift, while less polar phosphine chalconide compound induced small energy level shift at the interface. Therefore, the use of the highly polar interlayer was effective to improve the Voc of OSCs.
Autonomic nervous system effects on ventricular repolarization and RR interval variability during head-up tilt (2002)

Changes in ventricular repolarization (VR) were analyzed using 32-lead high spatial resolution ECGs (HSRE). FFT-derived low (0.04-0.15 Hz) and high (0.15-0.4 Hz) frequency powers (LF & HF, respectively) of RR interval variability (RRIV) and their ratio were used to assess changes in the autonomic activity elicited by 70/spl deg/ tilt in 9 patients with structural heart disease (SHD) and 19 control (CON) subjects. Heart rate (HR) increased with tilt in CON and SHD. HF and LF/HF increased with tilt in CON, but not in SHD. Peak and mean T-wave amplitudes declined with tilt in CON but not in SHD. In contrast to CON subjects, RRIV and VR failed to change in SHD patients despite increased HR. This suggests preserved tonic but reduced rhythmic autonomic nervous system activity (ANSA) related modulation of HR, and reduced ANSA effects on VR.