gebhart/patrepeval huggingface dataset:
Enhancement of immune cellular agglutination by use of an avidin-biotin system. (1979)

Classically, one can increase the titer of an agglutinating or first antibody with an antiglobulin or second antibody. We have used an avidin-biotin system in place of antiglobulin to similarly extend the agglutination by an initial anticellular antibody. Erythrocytes were agglutinated by adding in succession, caproylamidobiotin-antibody, avidin, and extender (caproylamidobiotin-macromolecule). The macromolecules evaluated as extenders, in order of increasing potency, were fibrinogen, albumin, succinylated polylysine, and ribonuclease A. From comparative testing, we found that antiglobulin raised the titer of antibody from 2560 to 20,480, and the avidin-biotin tool raised the titer of caproylamidobiotin-antibody from 2560 to 10,240 without extender and to 81,820 with an extender of caproylamidobiotin-ribonuclease. Thus noncovalent extenders add to the capability of the avidin-biotin system to facilitate and substitute for an antibody.
Supplementary Table S2 from Functional Gene Expression Analysis Uncovers Phenotypic Switch in Aggressive Uveal Melanomas (2023)

Supplementary Table S2 from Functional Gene Expression Analysis Uncovers Phenotypic Switch in Aggressive Uveal Melanomas
Probiotic Strain Lactobacillus casei BL23 Prevents Colitis-Associated Colorectal Cancer (2017)

The gut microbiota plays a major role in intestinal health, and an imbalance in its composition can lead to chronic gut inflammation and a predisposition to developing colorectal cancer (CRC). Currently, the use of probiotic bacteria represents an emerging alternative to treat and prevent cancer. Moreover, consumption of these beneficial bacteria may also favorably modulate the composition of the gut microbiota, which has been described in several studies to play an important role in CRC carcinogenesis. In this context, the aim of this study was to assess the protective effect of oral treatment with <i>Lactobacillus casei</i> BL23, a probiotic strain well known for its anti-inflammatory and anticancer properties. First, CRC was induced in C57BL6 mice by a single intraperitoneal injection with azoxymethane (8 mg/kg), followed by four courses of dextran sodium sulfate (2.5%) in drinking water that were separated by an adjustable recovery period. At the time of sacrifice (day 46), tumor incidence, histological scores, and epithelial proliferation were determined in colon samples. Our results show that <i>L. casei</i> BL23 significantly protected mice against CRC development; specifically, <i>L. casei</i> BL23 treatment reduced histological scores and proliferative index values. In addition, our analysis revealed that <i>L. casei</i> BL23 had an immunomodulatory effect, mediated through the downregulation of the IL-22 cytokine, and an antiproliferative effect, mediated through the upregulation of <i>caspase-7, caspase-9</i>, and <i>Bik</i>. Finally, <i>L. casei</i> BL23 treatment tended to counterbalance CRC-induced dysbiosis in mice, as demonstrated by an analysis of fecal microbiota. Altogether our results demonstrate the high potential of <i>L. casei</i> BL23 for the development of new, probiotic-based strategies to fight CRC.
A Comparative Analysis of Information Gathering Meetings Conducted by Novice Design Teams Across Multiple Design Project Stages (2021)

Abstract Designers often gather information, for instance through stakeholder or domain expert meetings, to understand their design problems and develop effective solutions. However, few previous studies have provided in-depth descriptions of novice engineering designers’ approaches to conducting information gathering meetings. In this preliminary study, we analyzed data from six capstone mechanical engineering design teams to identify the types of individuals from whom teams gathered information, when these meetings occurred, and how teams solicited information during meetings. Teams in our study exhibited a range of information gathering behaviors that aligned with recommended practices, particularly in their early meetings. We also observed relatively few instances of teams exhibiting behaviors that were less similar to recommended practices during their meetings. However, our findings revealed two key trends across teams that represented specific opportunities for improvement and that may reflect characteristic novice approaches to conducting information gathering meetings. First, teams explored domain experts’ perspectives in depth during meetings and met with additional domain experts to inform their projects. Teams' meetings with project partners contained few instances of deep exploratory information gathering behaviors in comparison. In addition, teams seemed to finalize design decisions during early design meetings and were less likely to conduct information gathering meetings during later design phases. The comprehensive descriptions of novice mechanical engineering designers’ approaches provided in our preliminary study provide an entry point for further investigations that can inform engineering training, tools, and pedagogy for conducting effective meetings.
RF pulse width modulator with reduced spectral regrowth (2017)

This paper presents the design of a RF pulse width (PW) modulator for switched-mode power amplification of varying envelope signals such as CDMA or OFDM. The low level of spectral regrow in RF PW modulated signal is ensured by RF pulse swallowing prevention. We proposed a manipulation of the baseband signal envelope in order to remove envelope values below a given amplitude threshold. We show that said envelope processing technique results in the simultaneous enhancement of the adjacent channel leakage ratio (ACLR) and achievement of the required error vector magnitude (EVM) performances. Experimental results for the LTE system with RF-PW modulation show that, with the proposed architecture, an additional 5-10 dB ACLR gain (respect to the conventional RF-PW modulator) can be achieved with EVM levels that completely satisfy 3GPP standard requirements.
Development of Strong Visible‐Light‐Absorbing Cyclometalated Iridium(III) Complexes for Robust and Efficient Light‐Driven Hydrogen Production (2022)

Weak light absorption of common Ir(III) complexes (e. g., using phenylpyridine as the ligand) has hindered their applications in photocatalytic hydrogen generation from water as an efficient photosensitizer. To address this issue, a series of cyclometalated Ir(III) complexes (Ir1-Ir5), featuring different electron-donating substituents to enhance the absorptivity, have been synthesized and studied as photosensitizers (PSs) for light-driven hydrogen production from water. Ir6-Ir7 were prepared as fundamental systems for comparisons. Electron donors, including 9-phenylcarbazole, triphenylamine, 4,4'-dimethoxytriphenylamine, 4,4'-di(N-hexylcarbazole)triphenylamine moieties were introduced on 6-(thiophen-2-yl)phenanthridine-based cyclometalating (C^N) ligands to explore the donor effect on the hydrogen evolution performance of these cationic Ir(III) complexes. Remarkably, Ir4 with 4,4'-dimethoxytriphenylamine achieved the highest turn-over number (TON) of 12 300 and initial turnover frequency (TOF<sub>i</sub> ) of 394 h<sup>-1</sup> , with initial activity (activity<sub>i</sub> ) of 547 000 μmol g<sup>-1</sup> h<sup>-1</sup> and initial apparent quantum yield (AQY<sub>i</sub> ) of 9.59 %, under the illumination of blue light-emitting diodes (LEDs) for 105 hours, which demonstrated a stable three-component photocatalytic system with high efficiency. The TON (based on n(H<sub>2</sub> )/n(PSr)) in this study is the highest value reported to date among the similar photocatalytic systems using Ir(III) complexes with Pt nanoparticles as catalyst. The great potential of using triphenylamine-based Ir(III) PSs in boosting photocatalytic performance has also been shown.
Blood Stem Cell Activity Is Arrested by Th1-Mediated Injury Preventing Engraftment following Nonmyeloablative Conditioning (2016)

T cells are widely used to promote engraftment of hematopoietic stem cells (HSCs) during an allogeneic hematopoietic cell transplantation. Their role in overcoming barriers to HSC engraftment is thought to be particularly critical when patients receive reduced doses of preparative chemotherapy and/or radiation compared with standard transplantations. In this study, we sought to delineate the effects CD4<sup>+</sup> cells on engraftment and blood formation in a model that simulates clinical hematopoietic cell transplantation by transplanting MHC-matched, minor histocompatibility-mismatched grafts composed of purified HSCs, HSCs plus bulk T cells, or HSCs plus T cell subsets into mice conditioned with low-dose irradiation. Grafts containing conventional CD4<sup>+</sup> T cells caused marrow inflammation and inhibited HSC engraftment and blood formation. Posttransplantation, the marrows of HSCs plus CD4<sup>+</sup> cell recipients contained IL-12-secreting CD11c<sup>+</sup> cells and IFN-γ-expressing donor Th1 cells. In this setting, host HSCs arrested at the short-term stem cell stage and remained in the marrow in a quiescent cell cycling state (G<sub>0</sub>). As a consequence, donor HSCs failed to engraft and hematopoiesis was suppressed. Our data show that Th1 cells included in a hematopoietic allograft can negatively impact HSC activity, blood reconstitution, and engraftment of donor HSCs. This potential negative effect of donor T cells is not considered in clinical transplantation in which bulk T cells are transplanted. Our findings shed new light on the effects of CD4<sup>+</sup> T cells on HSC biology and are applicable to other pathogenic states in which immune activation in the bone marrow occurs such as aplastic anemia and certain infectious conditions.
Constitutive expression of a groEL-related protein on the surface of human gamma/delta cells (2020)

Rabbit antibodies to hsp58 (P1), the human homologue of the Escherichia coli stress protein groEL, react specifically in indirect immunofluorescence and complement-dependent microcytoxicity experiments with a cell surface antigen expressed constitutively by T cell lines bearing gamma/delta receptors. This anti-hsp58-reactive antigen is not demonstrable on T cells that express alpha/beta receptors or on various cells that lack T cell receptors. Certain evidence was obtained to suggest that the target antigen on the surface of gamma/delta T cells is a approximately 77-kD protein distinct from intracellular hsp58 and known members of the hsp70 stress protein family. While the exact nature and significance of this anti-hsp58-reactive protein remain to be determined, these data may help to clarify the roles of groEL- related stress proteins and gamma/delta cells that recognize groEL homologous in immunologic defense against infection and in autoimmune disease.
Synthesis and Reactions of 3-Ethynyl-2-(triphenylphosphoimino)-1-azaazulenes (2007)

3-(Trimethylsilylethynyl)-and 3-(phenylethynyl)-2-(triphenylphosphoimino)-1-azaazulenes were synthesized by the Appel reaction of the corresponding 2-amino-3-ethynyl-1-azaazulenes.Reaction of 3-(phenylethynyl)-2-(triphenylphosphoimino)-1-azaazulene (8b) with Cu(OTf) 2 gave 3-(2-oxophenethyl)-2-(triphenylphosphoimino)-1-azaazulene (11), 3-(1,2-dioxophenethyl)-2-(triphenylphosphoimino)-1-azaazulene (12), and 2-amino-3-(1,2dioxophenethyl)-1-azaazulene ( 13).The structure of 13 was decided by X-ray structure analysis, and the structure of 12 was discussed by molecular orbital calculation.Reaction of 8b with aryl isocyanate in the presence of benzoyl peroxide gave 1,10-diazabenz[a]azulene derivative.
Analysis and dimensioning of credit-based flow control for the ABR service in ATM networks (2002)

ATM networks have shown to reveal difficulties in simultaneously guaranteeing the requested quality-of-service while efficiently utilizing all the available bandwidth for economic reasons. The ABR (available bit rate) service class was designed to fill the gap of bandwidth with less delay but loss sensitive traffic. Due to the bursty nature of higher-priority traffic and the resulting bandwidth fluctuations a flow control is required for ABR that avoids buffer overflow and cell loss. These demands can both only be supported by credit-based flow control. It offers immediate access to the full link bandwidth without a ramp-up time. However, though its behaviour is deterministic an analysis by queueing models is impossible. In this paper we treat stability, performance and dimensioning issues of a representative class of credit-based flow control protocols with a new stochastic Petri-net model. We use its formalism to prove its stability and study the utilization performance in all operation regions with it. This implies the proposed dimensioning of buffer sizes and parameters, which is an important task during connection admission.
Gastric Heterotopic Pancreas: Computed Tomography With Clinicopathologic Correlation (2017)

Asymptomatic heterotopic pancreas (HP) is benign and can be monitored long term without further intervention. Heterotopic pancreas is sometimes surgically resected because it can be difficult to distinguish HP from neoplastic submucosal masses such as gastrointestinal stromal tumors. Although it is not very common, HP should be considered in the differential diagnosis when diagnosing extramucosal gastric masses to avoid unnecessary intervention. We review gastric HP with pathologic correlation to help with diagnosing HP on computed tomography.
Molecular dynamics simulation of strong interaction mechanisms at wet interfaces in clay–polysaccharide nanocomposites (2014)

The thermodynamics of xyloglucan adsorption to montmorillonite clay in water was studied with molecular dynamics simulations.
INVESTIGATION OF STRUCTURES OF TETRAKIS(THIADIAZOLE)PORPHYRAZINE ON GOLD SUBSTRATE USING UHV-STM (2006)

It has been proposed that a bottom–up approach is necessary to make nanometer-scale devices. To improve the bottom–up approach, we need to observe and evaluate the structure and array of molecules on a substrate. Generally, the conformation of molecules at interfaces is critical in molecular devices. Numerous studies of phthalocyanine compounds have been done because of their high potential for applications in electro-optical and magnetic devices. Tetrakis(thiadiazole)porphyrazine (TTDPzH 2 ) is one of the molecules that has been synthesized to control intermolecular interactions. X-ray crystal analysis revealed a 2D close packing structure. However, the details of intermolecular interaction and molecule–substrate interaction in thin films are not completely understood. We have observed the structure and arrangement of molecules that reflect their structural characteristics by using a scanning tunneling microscope (STM). Molecules were deposited on gold substrates to a sub-monolayer thickness. STM images of monolayer thin films showed differences in the crystal structure between as-deposited and post-annealed films. STM images of sub-monolayer films showed small dots that are consistent with TTDPzH 2 molecule and cluster structures on the substrate terrace.
Impact of Digital Leadership on Kuwaiti hospitals’ Employees’ Performance (2022)

The study aimed to identify the Impact of Digital Leadership on Kuwaiti hospitals' employees' performance using a descriptive and analytical methodology. According to statistics from the Kuwaiti Ministry of Health, the study population consists of administrators in Kuwaiti hospitals, numbering 12006. A simple random sample commensurate with the study's objectives was drawn, numbering 372 administrators to distribute the study questionnaire to them, and 277 valid questionnaires were retrieved for analysis. It was also found that there is a statistically significant impact at the significance level (a=0.05) of digital leadership with its dimensions (innovation, persuasion, and knowledge) on Kuwaiti hospitals' employees' performance. The researchers recommended that it is essential to strengthening the factors that lead to enhancing the concept of digital leadership under its crucial elements in the work environment. as well as paying attention to the priorities of the impact of the digital leadership elements on the performance of employees to obtain a practical effect.
Upcycled synthesis and extraction of carbon‐encapsulated iron carbide nanoparticles for gap Plasmon applications in perovskite solar cells (2023)

Abstract An effective method for obtaining large amounts of metal nanoparticles (NPs) encapsulated by carbon layers through upcycling from floating‐catalyst aerosol chemical vapor‐deposited carbon nanotubes is demonstrated. NPs with diameters of less than 20 μm are selectively extracted from the synthesized carbon assortments through sonication, centrifugation, and filtration. The particles show an aggregation behavior owing to the π–π interaction between the graphitic carbon shells surrounding the iron carbides. By controlling the degree of the aggregation and arrangement, the light scattering by the gap‐surface plasmon effect in perovskite solar cells is maximized. Application of the NPs to the devices increased the power conversion efficiency from 19.71% to 21.15%. The short‐circuit current density ( J SC ) trend over the particle aggregation time accounts for the plasmonic effect. The devices show high stability analogue to the control devices, confirming that no metal‐ion migration took place thanks to the encapsulation. image
Cryopreserved Human Natural Killer Cells Exhibit Potent Antitumor Efficacy against Orthotopic Pancreatic Cancer through Efficient Tumor-Homing and Cytolytic Ability (2019)

Pancreatic cancer is known to be highly aggressive, and desmoplasia-induced accumulation of extracellular matrix (ECM), which is a hallmark of many pancreatic cancers, severely restricts the therapeutic efficacy of both immunotherapeutics and conventional chemotherapeutics due to the ECM functioning as a major physical barrier against permeation and penetration. In the case of cell-based immunotherapeutics, there are several other bottlenecks preventing translation into clinical use due to their biological nature; for example, poor availability of cell therapeutic in a readily usable form due to difficulties in production, handling, shipping, and storage. To address these challenges, we have isolated allogeneic natural killer (NK) cells from healthy donors and expanded them <i>in vitro</i> to generate cryopreserved stocks. These cryopreserved NK cells were thawed to evaluate their therapeutic efficacy against desmoplastic pancreatic tumors, ultimately aiming to develop a readily accessible and mass-producible off-the-shelf cell-based immunotherapeutic. The cultured NK cells post-thawing retained highly pure populations of activated NK cells that expressed various activating receptors and a chemokine receptor. Furthermore, systemic administration of NK cells induced greater <i>in vivo</i> tumor growth suppression when compared with gemcitabine, which is the standard chemotherapeutic used for pancreatic cancer treatment. The potent antitumor effect of NK cells was mediated by efficient tumor-homing ability and infiltration into desmoplastic tumor tissues. Moreover, the infiltration of NK cells led to strong induction of apoptosis, elevated expression of the antitumor cytokine interferon (IFN)-γ, and inhibited expression of the immunosuppressive transforming growth factor (TGF)-β in tumor tissues. Expanded and cryopreserved NK cells are strong candidates for future cell-mediated systemic immunotherapy against pancreatic cancer.
Enzyme Hydrolysates fromStichopus horrensas a New Source for Angiotensin-Converting Enzyme Inhibitory Peptides (2012)

Stichopus horrens flesh was explored as a potential source for generating peptides with angiotensin-converting enzyme (ACE) inhibitory capacity using 6 proteases, namely alcalase, flavourzyme, trypsin, papain, bromelain, and protamex. Degree of hydrolysis (DH) and peptide profiling (SDS-PAGE) of Stichopus horrens hydrolysates (SHHs) was also assessed. Alcalase hydrolysate showed the highest DH value (39.8%) followed by flavourzyme hydrolysate (32.7%). Overall, alcalase hydrolysate exhibited the highest ACE inhibitory activity (IC(50) value of 0.41 mg/mL) followed by flavourzyme hydrolysate (IC(50) value of 2.24 mg/mL), trypsin hydrolysate (IC(50) value of 2.28 mg/mL), papain hydrolysate (IC(50) value of 2.48 mg/mL), bromelain hydrolysate (IC(50) value of 4.21 mg/mL), and protamex hydrolysate (IC(50) value of 6.38 mg/mL). The SDS-PAGE results showed that alcalase hydrolysate represented a unique pattern compared to others, which yielded potent ACE inhibitory peptides with molecular weight distribution lower than 20 kDa. The evaluation of the relationship between DH and IC(50) values of alcalase and flavourzyme hydrolysates revealed that the trend between those parameters was related to the type of the protease used. We concluded that the tested SHHs would be used as a potential source of functional ACE inhibitory peptides for physiological benefits.
RF CMOS for microwave and MM-wave applications (2006)

RF CMOS is gaining significant momentum as the technology of choice for implementing product designs in the 1-10GHz band. With scaling pushing f <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">T</sub> and f <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">MAX</sub> of FET's beyond 300GHz and integration of back-end-of-line (BEOL) conducive to low-loss passives, CMOS is poised to address application needs in the X, K and V bands
Development and applications of oncolytic Maraba virus vaccines (2018)

Oncolytic activity of the MG1 strain of the Maraba vesiculovirus has proven efficacy in numerous preclinical cancer models, and relied not only on a direct cytotoxicity but also on the induction of both innate and adaptive antitumor immunity. To further expand tumor-specific T-cell effector and long-lasting memory compartments, we introduced the MG1 virus in a prime-boost cancer vaccine strategy. To this aim, a replication-incompetent adenoviral [Ad] vector together with the oncolytic MG1 have each been armed with a transgene expressing a same tumor antigen. Immune priming with the Ad vaccine subsequently boosted with the MG1 vaccine mounted tumor-specific responses of remarkable magnitude, which significantly prolonged survival in various murine cancer models. Based on these promising results, we validated the safety profile of the Ad:MG1 oncolytic vaccination strategy in nonhuman primates and initiated clinical investigations in cancer patients. Two clinical trials are currently under way (NCT02285816; NCT02879760). The present review will recapitulate the discoveries that led to the development of MG1 oncolytic vaccines from bench to bedside.