gebhart/patrepeval huggingface dataset:
Impact of Parallel Micro-Engineered Stent Grooves on Endothelial Cell Migration, Proliferation, and Function (2012)

Background— Stent luminal surface characteristics influence surface endothelialization. We hypothesize that luminal stent microgrooves created in the direction of coronary flow accelerate endothelial cell migration, resulting in lower levels of neointimal formation. Methods and Results— Surface coverage efficiency was evaluated in vitro by allowing human aortic endothelial cells (HAEC) to migrate onto microgrooved (G) or smooth (NG) surfaces. HAEC functionality was assessed by proliferation rate, apoptosis rate, nitric oxide production, and inflammatory markers TNF-α and VCAM-1 expression. Early endothelialization and restenosis studies were performed using the porcine coronary injury model. Stainless steel stents of identical design with (GS) and without (NGS) luminal microgrooves were used. The commercially available Multi-Link Vision (MLVS) stent of identical design was used as a control. The degree of GS and NGS surface endothelialization was compared at 3 days. Biocompatibility and tissue response outcomes were evaluated at 28 days. The in vitro study demonstrated that at 7 days the presence of surface microgrooves increased HAEC migration distance &gt;2-fold. Cell proliferation rate and nitric oxide production were increased and apoptosis rate was decreased. There was no difference in inflammatory marker expression. At 3 days, coronary artery stent endothelialization was significantly increased in GS compared with NGS (81.3% versus 67.5%, P =0.0002). At 28 days, GS exhibited lower neointimal thickness compared with either NGS (21.1%, P =0.011) or MLVS (40.8%, P =0.014). Conclusion— Parallel microgrooves on coronary stent luminal surfaces promote endothelial cell migration and positively influence endothelial cell function, resulting in decreased neointimal formation in the porcine coronary injury model.
Discovery and Optimization of Dibenzodiazepinones as Allosteric Mutant-Selective EGFR Inhibitors (2019)

Allosteric kinase inhibitors represent a promising new therapeutic strategy for targeting kinases harboring oncogenic driver mutations in cancers. Here, we report the discovery, optimization, and structural characterization of allosteric mutant-selective EGFR inhibitors comprising a 5,10-dihydro-11<i>H</i>-dibenzo[<i>b</i>,<i>e</i>][1,4]diazepin-11-one scaffold. Our structure-based medicinal chemistry effort yielded an inhibitor (<b>3</b>) of the EGFR(L858R/T790M) and EGFR(L858R/T790M/C797S) mutants with an IC<sub>50</sub> of ∼10 nM and high selectivity, as assessed by kinome profiling. Further efforts to develop allosteric dibenzodiazepinone inhibitors may serve as the basis for new therapeutic options for targeting drug-resistant EGFR mutations.
Unique challenges for glioblastoma immunotherapy—discussions across neuro-oncology and non-neuro-oncology experts in cancer immunology. Meeting Report from the 2019 SNO Immuno-Oncology Think Tank (2020)

Cancer immunotherapy has made remarkable advances with over 50 separate Food and Drug Administration (FDA) approvals as first- or second-line indications since 2015. These include immune checkpoint blocking antibodies, chimeric antigen receptor-transduced T cells, and bispecific T-cell-engaging antibodies. While multiple cancer types now benefit from these immunotherapies, notable exceptions thus far include brain tumors, such as glioblastoma. As such, it seems critical to gain a better understanding of unique mechanistic challenges underlying the resistance of malignant gliomas to immunotherapy, as well as to acquire insights into the development of future strategies. An Immuno-Oncology Think Tank Meeting was held during the 2019 Annual Society for Neuro-Oncology Scientific Conference. Discussants in the fields of neuro-oncology, neurosurgery, neuro-imaging, medical oncology, and cancer immunology participated in the meeting. Sessions focused on topics such as the tumor microenvironment, myeloid cells, T-cell dysfunction, cellular engineering, and translational aspects that are critical and unique challenges inherent with primary brain tumors. In this review, we summarize the discussions and the key messages from the meeting, which may potentially serve as a basis for advancing the field of immune neuro-oncology in a collaborative manner.
ChemInform Abstract: Efficient Mn—Cu and Mn—Co—TEMPO Catalyzed Oxidation of Alcohols into Aldehydes and Ketones by Oxygen under Mild Conditions. (2001)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Rheological Investigation as Tool to Assess Physicochemical Stability of a Hyaluronic Acid Dermal Filler Cross-Linked with Polyethylene Glycol Diglycidyl Ether and Containing Calcium Hydroxyapatite, Glycine and L-Proline (2022)

(1) Background: Dermal fillers are commonly used in aesthetic practice and their rheological characterization is of much interest today, as well as the stability study of the finished formula against external stimuli of a different nature (biological and physicochemical). Rheological tools have been exploited to characterize the physiochemical behaviour of a hyaluronic acid (HA) based dermal filler subjected to different thermal conditions over time. The collected results provide an index of its rheological stability. (2) Methods: After a preliminary Amplitude sweep test, the Frequency sweep test was performed in order to study the stability of a HA dermal filler cross-linked with Polyethylene Glycol Diglycidyl Ether (PEGDE) and containing Calcium Hydroxyapatite (CaHA), Glycine and L-Proline subjected to different conditions. Also, a shear rate ramp test was performed in order to investigate the filler's flow behavior. (3) Results and Conclusions: G' (elastic modulus), G'' (viscous modulus) and consequentially tan δ (tangent of the phase angle) show a similar trend at different thermal conditions, underlining that the product is not affected by the storage conditions. The viscosity of the dermal filler decreases with an increasing shear rate, so a non-Newtonian shear thinning pseudoplastic behavior was demonstrated in all tested conditions.
Analysis of defibrillation efficacy from myocardial voltage gradients with finite element modeling (1999)

Increasing defibrillation efficacy by lowering the defibrillation threshold (DFT) is an important goal in positioning implantable cardioverter-defibrillator electrodes. Clinically, the DFT is difficult to estimate noninvasively. It has been suggested that the DFT relates to the myocardial voltage gradient distribution, but this relation has not been quantitatively demonstrated. We analyzed the relation between the experimentally measured DFT's and the simulated myocardial voltage gradients provided by finite element modeling. We performed a series of experiments in 11 pigs to measure the DFT's, and created and solved three-dimensional subject-specific finite element models to assess the correlation between the computed myocardial voltage gradient histograms and the DFT's. Our data show a statistically significant correlation between the DFT and the left ventricular voltage gradient distribution, with the septal region being more significant (correlation coefficient of 0.74) than other myocardial regions. The correlation between the DFT and the right ventricular and the atrial voltage gradient, on the other hand, is not significant.
High-Throughput Chromatographic Separation of Oligonucleotides: A Proof of Concept Using Ultra-Short Columns (2023)

Ion-pairing reversed-phase liquid chromatography (IP-RPLC) is the reference separation technique for characterizing oligonucleotides (ONs) and their related impurities. The aim of this study was to better understand the retention mechanism of ONs, evaluate the applicability of the linear solvent strength (LSS) retention model, and explore the potential of ultra-short columns having a length of only 5 mm for the separation of model ONs. First, the validity of the LSS model was evaluated for ONs having sizes comprised between 3 and 30 kDa, and the accuracy of retention time predictions was assessed. It was found that ONs in IP-RPLC conditions follow an "on-off" elution behavior, despite a molecular weight lower than that of proteins. For most linear gradient separation conditions, a column length between 5 and 35 mm was found to be appropriate. Ultra-short columns of only 5 mm were therefore explored to speed up separations by considering the impact of the instrumentation on the efficiency. Interestingly, the impacts of injection volume and post-column connection tubing on peak capacity were found to be negligible. Finally, it was demonstrated that longer columns would not improve selectivity or separation efficiency, but baseline separation of three model ONs mixtures was enabled in as little as 30 s on the 5 mm column. This proof-of-concept work paves the way for future investigations using more complex therapeutic ONs and their related impurities.
Inhibition of long‐term potentiation by interleukin‐8: Implications for human immunodeficiency virus‐1‐associated dementia (2002)

Human immunodeficiency virus type 1 (HIV-1)-infected mononuclear phagocytes (MP; brain macrophages and microglia) secrete a number of toxic factors that affect the pathogenesis of HIV-1-associated dementia (HAD). The identification and relative role of each MP toxin for neuronal dysfunction during HAD are not well understood. Interleukin-8 (IL-8), a CXC chemokine involved in leukocyte activation and chemotaxis, is constitutively produced by MP, and elevated levels of IL-8 mRNA were detected in the brains of patients with HIV-1 encephalitis (HIVE) by both ribonuclease protection assays and real-time PCR. To determine the role that IL-8 might play in the neuronal dysfunction in HAD, we studied its effect on synaptic transmission and plasticity in the CA1 region of hippocampus, the seat of learning and memory. Bath application of IL-8 (50 ng/ml) to rat hippocampal slices had no effect on basal synaptic transmission. However, IL-8 was shown to inhibit long-term potentiation (LTP) in a concentration-dependent manner. In control and IL-8-treated slices, the LTP magnitudes were 167.8% +/- 11.9% (mean +/- SE; n = 17) and 122.2% +/- 16.2% of basal levels (n = 13), respectively. These differences were statistically significant (P < 0.05). Preincubation of hippocampal slices with a monoclonal CXCR2 antibody (2 microg/ml) but not control IgG (2 microg/ml) blocked IL-8-induced inhibition of LTP. The expression of CXCR2 receptors in the CA1 region was shown by Western blot assays. The induction of IL-8 in HAD, its inhibition of LTP, and the expression of its receptor, CXCR2, in the hippocampus all suggest that it plays a role in the cognitive dysfunction associated with HAD.
Advanced Ground Systems Maintenance Enterprise Architecture Project (2015)

The project implements an architecture for delivery of integrated health management capabilities for the 21st Century launch complex. The delivered capabilities include anomaly detection, fault isolation, prognostics and physics based diagnostics.
Safe integration of depletion mode SiC VJFET power modules into a commercial motor drive (2017)

This paper addresses the replacement of enhancement mode Si IGBTs with depletion mode SiC vertical junction field effect transistors (VJFETs) in a commercial motor drive in current production. A Yaskawa A1000 CIMR-AU4A0088FAA motor drive was analyzed and found to have reliability and safety features that can be exploited to introduce normally on SiC transistors with no loss in safety or reliability as compared to the baseline configuration using silicon IGBT power modules. It is found that by utilizing the components and basic architecture of the original drive that are intended to ensure single-point-of-failure reliability with IGBTs, the SiC JFETs can be safely integrated. As a result, a significant efficiency increase of 1 to 2 percentage points over the same motor drive with IGBT modules was measured. In terms of the inverter losses, a mean reduction of 36% across the operating range of the drive was observed.
INFLUENCE OF LARGE-SCALE LOW- AND HIGH-SPEED STRUCTURES ON A TURBULENT BOUNDARY LAYER (2017)

Direct numerical simulation data of a turbulent boundary layer (Reτ ~ 1000) are used to explore the influences of large-scale structures on the near-wall vortical motions. The streamwise swirling strengths (λx) are conditionally sampled in terms of the large-scale streamwise velocity fluctuations (ul). In the near-wall region, the amplitudes of λx diminish under negative ul rather than under positive ul. This behaviour is due to the near-wall spanwise motions within the footprints of largescale structures, which are the congregative and dispersive motions induced by the outer large-scale low-speed and high-speed structures. The intense dispersive motions under the footprint of positive ul strengthen the smallscale spanwise velocity fluctuations (ws) close to the wall compared to ws affected by the congregative motions of negative ul. The wall-normal velocity components were attenuated or amplified around the modulated vortical motions, which leads to the dependence of λx on the large scales. To quantify the contribution of the modulated vortical motions, we use the decomposition method of Yoon et al. (2016), which is the relationship between the velocity-vorticity correlations and the skin-friction coefficient (Cƒ). In the near-wall region, intense values of 〈−wωy〉, related to change-of-scale effect due to the vortex stretching force, are observed for the positive-ul event. The skin friction induced by the amplified vortical motions within ul+ > 2 carries 15% of the total skin friction generated by the change-of-scale effect.
The centrosomin CM2 domain is a multi-functional binding domain with distinct cell cycle roles (2017)

Abstract The centrosome serves as the main microtubule-organizing center in metazoan cells, yet despite its functional importance, little is known mechanistically about the structure and organizational principles that dictate protein organization in the centrosome. In particular, the protein-protein interactions that allow for the massive structural transition between the tightly organized interphase centrosome and the highly expanded matrix-like arrangement of the mitotic centrosome have been largely uncharacterized. Among the proteins that undergo a major transition is the Drosophila melanogaster protein centrosomin that contains a conserved carboxyl terminus motif, CM2. Recent crystal structures have shown this motif to be dimeric and capable of forming an intramolecular interaction with a central region of centrosomin. Here we use a combination of in-cell microscopy and in vitro oligomer assessment to show that dimerization is not necessary for CM2 recruitment to the centrosome and that CM2 alone undergoes a significant cell cycle dependent rearrangement. We use NMR binding assays to confirm this intramolecular interaction and show that residues involved in solution interactions are consistent with the published crystal structure and identify L1137 as critical for binding. Additionally, we show for the first time an in vitro interaction of CM2 with the Drosophila pericentrin-like-protein that exploits the same set of residues as the intramolecular interaction. Furthermore, NMR experiments reveal a calcium sensitive interaction between CM2 and calmodulin. Although unexpected because of sequence divergence, this suggests that centrosomin-mediated assemblies, like the mammalian pericentrin, may be calcium regulated. From these results we suggest a model where during interphase CM2 interacts with pericentrin-like-protein to form a layer of centrosomin around the centriole wall and that at the onset of mitosis this population acts as a nucleation site of intramolecular centrosomin interactions that support the expansion into the metaphase matrix.
Fatigue Strength of Stampable Short Carbon Fiber-Reinforced Polyamide Composites (2016)

The static and fatigue behaviors of stampable discontinuous carbon fiber-reinforced polyamide (DCF/PA) composite have been examined. Static tension and compression tests are first performed at different temperatures (RT, 70°C and 130°C), respectively, to quantify the temperature dependence of basic mechanical properties of the composite. Then, constant amplitude fatigue tests are carried out for different stress ratios (0.1, χ, -1 and 10) at different temperatures (RT, 70°C and 130°C), respectively. The fatigue test results demonstrate that the S-N relationship of the discontinuous fiber composite significantly depends on stress ratio, and fatigue degradation occurs most rapidly at the critical stress ratio in line with the observations made thus far for continuous carbon fiber composites. Finally, the anisomorphic constant fatigue life diagram approach developed for continuous carbon fiber composites is applied to the DCF/PA composite, and it is proved that this method can successfully be used to predict the fatigue life values of the discontinuous carbon fiber-reinforced composite at different stress ratios as well.
Enhanced Data Return from Lunar Farside using RF-Optical TT and C (2008)

Science return and high bandwidth communications are key issues to support the foreseen endeavours on spaceflights to the Moon and beyond. For a given mass, power consumption and volume, laser communications can offer an increase in TM bandwidth over classical RF technology allowing for a variety of new options, specifically to missions that require very large distances, such as to the Moon and even beyond. This increase in TM data rate allows for more raw scientific data to be sent back to Earth where data processing can be performed on ground. Enhanced sensing techniques could be used that generate more science data and access during flight could be faster. This paper presents an overview of proof of concept test results obtained for optical telemetry return in a field test campaign together with ESA in October 2007. Based on the results obtained, a sample mission is outlined that shows an optical relay on a HALO trajectory at lunar farside, using an integrated RF-optical TT&C transponder concept.
3.8 Effects of H2 Enrichment on Flame Stability and Pollutant Emissions for a Kerosene/Air Swirled Flame with an Aeronautical Injector (2020)

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<title>Ultrathin fiberscope for stereoscopic vision</title> (1993)

Endoscope for stereoscopic vision is in great demand for medical field to diagnose and treat disease parts of patients more precisely. The authors are convinced that the silica-based imagefiber is suitable for making a thinner fiberscope for stereoscopic vision and have developed an ultrathin fiberscope with silica-based imagefibers for stereoscopic vision. The diameter of fiberscope is only 2 mm with two transmitting systems that consist of two imagefibers with 10000 pixels and lightguide for illumination and objective lenses. This fiberscope for stereoscopic vision can be used for the microsurgery, such as the laparoscopic surgery and the thoracoscopic surgery required for the thin diameter.
The role of human RecQL4 helicase protein interactions in genomic stability (2005)

1789 The unique biopolymer poly(ADP-ribose) (PAR) is synthesized by members of the poly(ADP-ribose) polymerase (PARP) family as a posttranslational modification of many cellular target proteins. PARP-1, a major PAR synthetic activity, is activated in response to DNA strand breaks. Once synthesized, PAR is rapidly degraded by action of poly(ADP-ribose) glycohydrolase (PARG). Cellular PAR metabolism modulates cellular responses to genotoxic stress by stimulating DNA repair or inducing cell death and is therefore an important factor for the maintenance of genomic integrity. Reduced poly(ADP-ribosyl)ation capacity, e.g. in PARP-1-/- mice, leads to a decrease in genomic stability and to an increase in tumor formation. In addition to PARP-1 and PARP-2, which are DNA strand break activated, numerous genes code for PARP enzymes that catalyze PAR synthesis in both nuclear and extranuclear compartments of the cell independent of the presence of DNA damage. Only one gene coding for PARG has been identified in mammalian cells. We recently described the human PARG gene structure (Meyer et al., Gene, 314: 181-190, 2003) and have shown that several cellular PARG protein isoforms of 111kDa (hPARG111), 102 kDa (hPARG102) and 99 kDa (hPARG99) are synthesized by translation of alternatively spliced messages (Meyer-Ficca et al., Exp Cell Res, 297: 521-532, 2004). The different isoforms localize to different cellular compartments and are therefore able to function in PAR homeostasis throughout the cell: hPARG111 is nuclear, while hPARG102 and hPARG99 appear to be cytoplasmic. Here we describe the detection of an additional human PARG splice variant coding for a 60 kDa protein (hPARG60), which also has a cytoplasmic subcellular distribution. To assess the functions of the different PARG isoforms in more detail, constructs allowing for the overexpression of fusion proteins of green fluorescent protein to the PARG isoforms were generated. Transient overexpression of these proteins in HEK293 cells increased the total cellular PAR degrading activity by 4-5 fold. The accumulation of PAR after DNA damage was markedly reduced. Overexpression of the longer PARG isoforms (hPARG111 and hPARG102) in HeLa cells induced distinct changes in nuclear morphology, while overexpression of the 60 kDa PARG isoform was cytotoxic. These finding support the concept that PAR homeostasis involving the coordination of cellular PARPs and PARGs play important roles not only in cellular responses to DNA damage, but also for the maintenance of genomic integrity in the absence of genotoxic stress. (Supported in part by NIH Grant CA-43894)
Validation of the Mayo Hip Score: construct validity, reliability and responsiveness to change (2016)

Mayo Hip Score is valid, sensitive to change and associated with future risk of revision surgery in patients with primary THA.
Suitability of ITU-R P.1546 propagation predictions for allocating LTE SDL with GE06 (2017)

The compatibility of Geneva 2006 frequency plan (GE06) assignments and allocations for digital terrestrial television (DTT) is determined using ITU-R P.1546 propagation prediction method. The aim of this paper is to study the suitability of using ITU-R P.1546 propagation predictions for allocating Long Term Evolution (LTE) Supplemental Downlink (SDL) to broadcast Audiovisual Media Services (AVMS) in the UHF TV band. This paper presents a field measurement campaign to study the accuracy of P.1546 propagation predictions for LTE base station signals in the close vicinity of the LTE base station, where DTT reception interference and overloading events are most likely to occur. The ITU-R P.1546 prediction signal strength in general was higher than the measured field strength, which means that using ITU-R P.1546 propagation predictions could thus overestimate the probability of interference events to DTT reception. The paper also studies use of more sophisticated site-specific propagation prediction methods, but the considered short-range propagation paths were found to be very difficult to predict regardless of the used method. The location-by-location analysis indicates that the propagation predictions are less accurate in the locations where the line-of-sight (LOS) or first Fresnel zone are obstructed.