gebhart/patrepeval huggingface dataset:
Monocyte‐derived dendritic cells from HCV‐infected patients transduced with an adenovirus expressing NS3 are functional when stimulated with the TLR3 ligand poly(I:C) (2008)

Dendritic cells (DC) transfected with an adenovirus encoding hepatitis C virus (HCV) NS3 protein (AdNS3) induce potent antiviral immune responses when used to immunize mice. However, in HCV infected patients, controversial results have been reported regarding the functional properties of monocyte-derived DC (MoDC), a cell population commonly used in DC vaccination protocols. Thus, with the aim of future vaccination studies we decided to characterize MoDC from HCV patients transfected with AdNS3 and stimulated with the TLR3 ligand poly(I:C). Phenotypic and functional properties of these cells were compared with those from MoDC obtained from uninfected individuals. PCR analysis showed that HCV RNA was negative in MoDC from patients after the culture period. Also, phenotypic analysis of these cells showed lower expression of CD80, CD86, and CD40, but similar expression of HLA-DR molecules as compared to MoDC from uninfected individuals. Functional assays of MoDC obtained from patients and controls showed a similar ability to activate allogeneic lymphocytes or to produce IL-12 and IL-10, although lower IFN-alpha levels were produced by cells from HCV patients after poly(I:C) stimulation. Moreover, both groups of MoDC induced similar profiles of IFN-gamma and IL-5 after stimulation of allogeneic T-cells. Finally, migration assays did not reveal any difference in their ability to respond to CCL21 chemokine. In conclusion, MoDC from HCV patients are functional after transduction with AdNS3 and stimulation with poly(I:C). These findings suggest that these cells may be useful for therapeutic vaccination in chronic HCV infection.
ChemInform Abstract: Orbital Distortion in Dibenzobicyclo(2.2.2)octatrienes. Biased Epoxidation and Dihydroxylation of the Olefin Moiety. (1993)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Supplementary Movie 10 from Ovarian Cancer Spheroids Use Myosin-Generated Force to Clear the Mesothelium (2023)

&lt;p&gt;File Type: .mov; File Size: 13 MB.&lt;/p&gt;
Total Synthesis of Immunosuppressants:  Unified Strategies Exploiting Dithiane Couplings and σ-Bond Olefin Constructions (1998)

ADVERTISEMENT RETURN TO ISSUEPREVArticleTotal Synthesis of Immunosuppressants: Unified Strategies Exploiting Dithiane Couplings and σ-Bond Olefin Constructions†Amos B. Smith, Stephen M. Condon, and John A. McCauleyView Author Information Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104 Cite this: Acc. Chem. Res. 1998, 31, 1, 35–46Publication Date (Web):January 20, 1998Publication History Received8 July 1997Published online20 January 1998Published inissue 20 January 1998https://pubs.acs.org/doi/10.1021/ar960289ihttps://doi.org/10.1021/ar960289iresearch-articleACS PublicationsCopyright © 1998 American Chemical SocietyRequest reuse permissionsArticle Views1084Altmetric-Citations85LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Alcohols,Coupling reactions,Ethers,Hydrocarbons,Vinyl Get e-Alerts
Bidentate Phosphine–Phosphoramidite Ligands of the BettiPhos Family for Rh‐Catalyzed Asymmetric Hydrogenation (2017)

Phosphine–phosphoramidites comprising a stereogenic phosphorus atom at the phosphoramidite moiety and a Betti base as chiral backbone have been synthesized. Individual diastereomers have been benchmarked as ligands in the Rh‐catalyzed asymmetric hydrogenation of dimethyl itaconate to study the interplay of the different elements of chirality. The privileged diastereomer was applied to Rh‐catalyzed asymmetric hydrogenation of several functionalized olefins, leading to high enantioselectivities between 91 and 97 % ee , which confirms the effectiveness of the BettiPhos ligand family in asymmetric catalysis.
METHODS OF SCALP REVISION FOR DEEP BRAIN STIMULATOR HARDWARE (2008)

Stimulation of various deep brain targets helps patients with a wide range of diseases. In the future, with continued refinement, hardware complications can be minimized. Until then, novel approaches need to be developed to save DBS systems and provide symptomatic relief to patients.
Secreted Antigens and Immune Responses to <i>Mycobacterium tuberculosi</i><i>s</i> (1997)

Recent information from several laboratories points to proteins secreted from live Mycobacterium tuberculosis as being involved in protective immunity. We have studied protein release from M. tuberculosis during growth and have defined a short-term culture filtrate enriched with secreted antigens and with a minimal content of autolytic products. Several components in this filtrate have been characterized and are presently being studied in animal models of tuberculosis. In the mouse model, protection against tuberculosis is mediated by a long-lived population of memory T cells directed against secreted molecules, and the antigens ESAT-6 and the 85 complex are important key targets for this response. The specificity of the protective immune response in humans is less well defined, but our studies indicate that antigen-specific CD4+ cytotoxic T cells and memory T cells secreting interferon-γ (IFN-γ) are involved. The IFN-γ response is directed against a range of the culture filtrate proteins with a preferential recognition of low molecular mass antigens.
Structural Features and Superconducting Properties of As-Grown Y-Ba-Cu-O Films (1989)

The structural features of superconducting Y-Ba-Cu-O films prepared by reactive sputtering have been discussed in relation to the preparation conditions. Conductivity anisotropy and the anomalous diamagnetic response observed for the films have also been discussed.
Computer-Assisted Eye Examination VI. Identification and Correction of Errors in the Refractor III System for Subjective Examination (1978)

A computer-assisted subjective eye examination was administered to 80 patients. They were also examined in the conventional manual way. The results of the computer-assisted examination were compared with those obtained by conventional subjective methods to identify errors of hardware, software, and optometric flow charting and other problems. An 87.5% satisfactory prescription level was found for the distance prescription. The 12.5% unsatisfactory results are accounted for by several kinds of error. The percentage of unsatisfactory refractive results was greater for near than for distance measures because of special flow-chart and software problems, which were subsequently corrected. With implementation of the changes initiated as a result of this investigation, satisfactory refractive results approaching 95% can be expected.
OS0316 The relationships between the amounts of localized deformation and deformation twin type (2012)

In this study, the origin of amounts of localized strain in basal textured Magnesium alloy sheets was investigated. First, grid patterns were drawn on a top surface of sample by Focus Ion Beam system. And then, a distribution of crystal orientation from same area was observed by SEM/EBSD. From these methods, the relationship between the localized strain and crystal orientation was revealed. In generally, the amounts of strains are dependent on the crystal orientation, which means the facility of slip deformation. However, my results show that localized strain is dependent on the existence or absence of twins and twin types, and but not on the Schmid factor of basal slip. And I suggest that {1012} type has the roll of strain accommodation to satisfy the strain compatibility with neighbor grain in primary deformation stage and continue to cancel the strain of slip during deformation. So the grain with {10-12} type slightly deformed. On the other hand, {10-11} type has the roll of accommodation strain component to conform to externally applied stress. But {10-11} twin easily generate double twin. And the grain with double twin localized severely deformed and formed surface steps or cracks.
Disorders of branched chain amino acid metabolism (2016)

The three essential branched-chain amino acids (BCAAs), leucine, isoleucine and valine, share the first enzymatic steps in their metabolic pathways, including a reversible transamination followed by an irreversible oxidative decarboxylation to coenzyme-A derivatives. The respective oxidative pathways subsequently diverge and at the final steps yield acetyl- and/or propionyl-CoA that enter the Krebs cycle. Many disorders in these pathways are diagnosed through expanded newborn screening by tandem mass spectrometry. Maple syrup urine disease (MSUD) is the only disorder of the group that is associated with elevated body fluid levels of the BCAAs. Due to the irreversible oxidative decarboxylation step distal enzymatic blocks in the pathways do not result in the accumulation of amino acids, but rather to CoA-activated small carboxylic acids identified by gas chromatography mass spectrometry analysis of urine and are therefore classified as organic acidurias. Disorders in these pathways can present with a neonatal onset severe-, or chronic intermittent- or progressive forms. Metabolic instability and increased morbidity and mortality are shared between inborn errors in the BCAA pathways, while treatment options remain limited, comprised mainly of dietary management and in some cases solid organ transplantation.
Assessing limb apraxia in traumatic brain injury and spinal cord injury (2012)

People with traumatic brain injury (TBI) may demonstrate action planning disorders and limb apraxia. Many patients, who sustain a spinal cord injury (SCI), sustain a co-occurring TBI (11-29 percent of people with SCI) and therefore are at risk for limb apraxia. People with SCI and TBI (SCI/TBI) rely on powered assistive devices which amplify movement. Their ability to learn complex motor compensatory strategies, that is, limb praxis, is critical to function. We wished to identify methods of screening for apraxia in patients with SCI/TBI. We reviewed instruments available for limb praxis assessment, presenting information on psychometric development, patient groups tested, commercial/clinical availability, and appropriateness for administration to people with motor weakness. Our review revealed that insufficient normative information exists for apraxia assessment in populations comparable to SCI/TBI patients who are typically young adults at the time of injury. There are few apraxia assessment instruments which do not require a motor response. Non-motoric apraxia assessments would be optimal for patients with an underlying motor weakness.
Ab InitioCalculation of Coverage-Dependent Adsorption Properties of H on Pd(001) (1986)

The preferential adsorption sites, bond lengths, and vibration frequencies for H on the Pd (001) surface are determined by use of the ab initio pseudopotential local-orbital method. From the calculated total energies we derive a coverage-dependent absorption energy in very good agreement with experiment. We establish that at low coverages ($\ensuremath{\theta}\ensuremath{\le}1$), H occupies fourfold hollow sites. At higher coverages ($1&lt;\ensuremath{\theta}\ensuremath{\le}2$), islands of bridge-bonded H (local coverage $\ensuremath{\theta}=2$) are predicted to coexist with regions of H in hollow sites.
Caged Molecular Glues as Photoactivatable Tags for Nuclear Translocation of Guests in Living Cells (2018)

We developed dendritic caged molecular glues (<sup>Caged</sup>Glue-R) as tags for nucleus-targeted drug delivery, whose multiple guanidinium ion (Gu<sup>+</sup>) pendants are protected by an anionic photocleavable unit (butyrate-substituted nitroveratryloxycarbonyl; <sup>BA</sup>NVOC). Negatively charged <sup>Caged</sup>Glue-R hardly binds to anionic biomolecules because of their electrostatic repulsion. However, upon exposure of <sup>Caged</sup>Glue-R to UV light or near-infrared (NIR) light, the <sup>BA</sup>NVOC groups of <sup>Caged</sup>Glue-R are rapidly detached to yield an uncaged molecular glue (<sup>Uncaged</sup>Glue-R) that carries multiple Gu<sup>+</sup> pendants. Because Gu<sup>+</sup> forms a salt bridge with PO<sub>4</sub><sup>-</sup>, <sup>Uncaged</sup>Glue-R tightly adheres to anionic biomolecules such as DNA and phospholipids in cell membranes by a multivalent salt-bridge formation. When tagged with <sup>Caged</sup>Glue-R, guests can be taken up into living cells via endocytosis and hide in endosomes. However, when the <sup>Caged</sup>Glue-R tag is photochemically uncaged to form <sup>Uncaged</sup>Glue-R, the guests escape from the endosome and migrate into the cytoplasm followed by the cell nucleus. We demonstrated that quantum dots (QDs) tagged with <sup>Caged</sup>Glue-R can be delivered efficiently to cell nuclei eventually by irradiation with light.
Bidimensional Localization of Active Ultrasound Markers (2014)

International audience
Megaspeed Drive Systems: Pushing Beyond 1 Million r/min (2009)

The latest research in mesoscale drive systems is targeting rotational speeds toward 1 million r/min for a power range of 1-1 kW. Emerging applications for megaspeed drives ( <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">MegaNdrives</i> ) are to be found in future turbo compressor systems for fuel cells and heat pumps, generators/starters for portable nanoscale gas turbines, printed circuit board drilling and machining spindles, and electric power generation from pressurized gas flow. The selection of the machine type and the challenges involved in designing a machine for megaspeed operation such as the winding concepts, a mechanical rotor design capable of 1 000 000 r/min, the selection of magnetic materials for the stator, and the optimization concerning high-frequency losses and torque density are presented. Furthermore, a review of the advantageous inverter topologies, taking into account the extremely low stator inductance and possible high-speed bearing types such as ball bearings, air bearings, foil bearings, and magnetic bearings, are given. Finally, prototypes and experimental results originating from <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">MegaNdrive</i> research at Swiss Federal Institute of Technology Zurich are discussed and extreme temperature operation and power microelectricalmechanical system are identified as targets for future research.
Structural basis for ligand-mediated mouse GITR activation (2008)

Glucocorticoid-induced TNF receptor ligand (GITRL) is a member of the TNF super family (TNFSF). GITRL plays an important role in controlling regulatory T cells. The crystal structure of the mouse GITRL (mGITRL) was determined to 1.8-A resolution. Contrary to the current paradigm that all ligands in the TNFSF are trimeric, mGITRL associates as dimer through a unique C terminus tethering arm. Analytical ultracentrifuge studies revealed that in solution, the recombinant mGITRL exists as monomers at low concentrations and as dimers at high concentrations. Biochemical studies confirmed that the mGITRL dimer is biologically active. Removal of the three terminal residues in the C terminus resulted in enhanced receptor-mediated NF-kappaB activation than by the wild-type receptor complex. However, deletion of the tethering C-terminus arm led to reduced activity. Our studies suggest that the mGITRL may undergo a dynamic population shift among different oligomeric forms via C terminus-mediated conformational changes. We hypothesize that specific oligomeric forms of GITRL may be used as a means to differentially control GITR receptor signaling in diverse cells.
Helicity in Ropes of Chiral Nanotubes: Calculations and Observation (2012)

Even though isolated defect-free single-wall carbon nanotubes are straight, bundles of chiral single-wall carbon nanotubes are often helical according to our observations using high-resolution electron microscopy. The driving force for the formation of such helices is the energy gain associated with the optimum orientational alignment of neighboring nanotubes. Our total energy calculations allow us to analyze the torsional and bending stress components in helical nanotube ropes and specify under which conditions straight nanotube bundles gain energy upon forming a helix.
The effect of alendronate (Fosamax™) and implant surface on bone integration and remodeling in a canine model (2001)

Patients at high risk for osteoporosis and its associated morbidity, including postmenopausal women, are being pharmacologically managed to stabilize and improve bone mass. Alendronate sodium (Fosamax) is a commonly used antiresorptive agent effective in osteopenic women for reducing bone resorption, increasing bone density, and decreasing fracture incidence. With the increased incidence of alendronate-treated women who are undergoing hip replacement or fracture repair by prosthesis placement, data are needed to predict how alendronate affects host bone integration with uncemented surfaces. The aim of this study was to determine the effect of alendronate on new bone formation and attachment to implant surfaces in a normal and simulated estrogen-deficient, calcium-deficient canine model, using an implantable bone growth chamber. Alendronate did not affect host bone integration to surfaces commonly used in uncemented total joint arthroplasty, but there were significant differences dependent solely on the type of surface.
Supplementary Figure Legend from Oncogenic ERG Represses PI3K Signaling through Downregulation of IRS2 (2023)

&lt;p&gt;Figure Legend for supplementary figures&lt;/p&gt;
Effector CD8 T cells use different homing receptor/ligand interactions to enter subcutaneous and intraperitoneal B16 melanoma tumors (2016)

Abstract CD8 T cell infiltration into tumors is critical for controlling cancer and relies on interactions between homing receptors (HR) on CD8 T cells and ligands on the vasculature. However, the ligands expressed on tumor vasculature, and the receptor interactions required for entry into tumors are incompletely characterized. The HR ligands VCAM-1 and E-selectin were highly expressed on the vasculature of B16-F1 melanomas expressing the strong antigen ovalbumin (B16-OVA) and growing subcutaneously but were significantly decreased compared to inflamed skin vasculature. In contrast, the vasculature of intraperitoneal tumors expressed MadCAM-1, negligible E-selectin, and lower VCAM-1. However, MadCAM-1 was not displayed on the luminal surface. B16-F1 parental tumors lacking a strong antigen, and B16-OVA tumors grown in Rag1KO animals, expressed negligible VCAM-1 and MadCAM-1, suggesting that previously infiltrating activated adaptive immune cells drove their expression. VCAM-1 and CXCL9/10 enabled entry of CD8 T cell effectors expressing α4β1+ integrin and CXCR3 into both subcutaneous and peritoneal tumors, while E-Selectin enabled entry of E-Selectin ligand+ effectors preferentially into subcutaneous tumors. MadCAM-1 did not mediate entry of α4β7+ effectors into peritoneal tumors, in keeping with its lack of luminal expression. Disrupting immunosuppression with checkpoint blockade inhibitors may increase HR ligand expression on tumor vasculature and improve CD8 T cell entry into tumors.
GNSS reflectrometry using the L5 and E5a signals for remote sensing applications (2013)

Signals from the Global Navigation Satellite Systems, GNSS, can be viewed as a passive source of radiation and therefore be used for remote sensing purposes. When reaching a surface, for example the ocean, the signal is reflected off the surface. The reflected signal can be used together with the direct signal to obtain observables such as altitude and wind speed.
Decreased levels of UMP kinase as a mechanism of fluoropyrimidine resistance (2009)

5-Fluorouracil (5-FU) continues to be widely used for treatment of gastrointestinal cancers. Because many tumors show primary or acquired resistance, it is important to understand the molecular basis underlying the mechanism of resistance to 5-FU. In addition to its effect on thymidylate synthase inhibition and DNA synthesis, 5-FU may also influence RNA metabolism. Our previous studies revealed that colorectal cancer cells resistant to bolus 5-FU (HCT-8/4hFU) showed significantly decreased incorporation of the drug into RNA. Resistance to bolus 5-FU was associated with lower expression of UMP kinase (UMPK), an enzyme that plays an important role in the activation of 5-FU to 5-FUTP and its incorporation into RNA. Activities of other 5-FU-metabolizing enzymes (e.g., thymidine kinase, uridine phosphorylase, thymidine phosphorylase, and orotate phosphoribosyltransferase) remained unchanged between sensitive and resistant cell lines. Herein, we show that UMPK down-regulation in 5-FU-sensitive cells (HCT-8/P) induces resistance to bolus 5-FU treatment. Moreover, HCT-8/4hFU cells are even more cross-resistant to treatment with 5-fluorouridine, consistent with the current understanding of 5-fluorouridine as a RNA-directed drug. Importantly, colorectal cancer hepatic metastases isolated from patients clinically resistant to weekly bolus 5-FU/leucovorin treatment exhibited decreased mRNA expression of UMPK but not thymidylate synthase or dihydropyrimidine dehydrogenase compared with tumor samples of patients not previously exposed to 5-FU. Our findings provide new insights into the mechanisms of acquired resistance to 5-FU in colorectal cancer and implicate UMPK as an important mechanism of clinical resistance to pulse 5-FU treatment in some patients.
The automatic detection of the optic disc location in retinal images using optic disc location regression (2006)

The automatic detection of the position of the optic disc is an important step in the automatic analysis of retinal images. A method to detect the approximate position of the optic disc using kNN regression is presented. The method starts by building a regression model of the optic disc position. Using a prior vessel segmentation all vessel pixels are searched for those which are inside the optic disc according to the regression model. The regression output is blurred to handle noise. The point which is closest to the middle of the optic disc is chosen. The method was tested on 1000 screening images and was able to find the correct position in 99.9% of all cases.
The reification of metaphor as a design tool (2006)

Despite causing many debates in human-computer interaction (HCI), the term “metaphor” remains a central element of design practice. This article investigates the history of ideas behind user-interface (UI) metaphor, not only technical developments, but also less familiar perspectives from education, philosophy, and the sociology of science. The historical analysis is complemented by a study of attitudes toward metaphor among HCI researchers 30 years later. Working from these two streams of evidence, we find new insights into the way that theories in HCI are related to interface design, and offer recommendations regarding approaches to future UI design research.