gebhart/patrepeval huggingface dataset:
Increased Production of Xylanase by Expression of a Truncated Version of the xyn11A Gene from Nonomuraea flexuosa in Trichoderma reesei (2007)

We have previously shown that the Nonomuraea flexuosa Xyn11A polypeptides devoid of the carbohydrate binding module (CBM) have better thermostability than the full-length xylanase and are effective in bleaching of pulp. To produce an enzyme preparation useful for industrial applications requiring high temperature, the region encoding the CBM was deleted from the N. flexuosa xyn11A gene and the truncated gene was expressed in Trichoderma reesei. The xylanase sequence was fused to the T. reesei mannanase I (Man5A) signal sequence or 3' to a T. reesei carrier polypeptide, either the Man5A core/hinge or the cellulose binding domain (CBD) of cellobiohydrolase II (Cel6A, CBHII). The gene and fusion genes were expressed using the cellobiohydrolase 1 (cel7A, cbh1) promoter. Single-copy isogenic transformants in which the expression cassette replaced the cel7A gene were cultivated and analyzed. The transformants expressing the truncated N. flexuosa xyn11A produced clearly increased amounts of both the xylanase/fusion mRNA and xylanase activity compared to the corresponding strains expressing the full-length N. flexuosa xyn11A. The transformant expressing the cel6A CBD-truncated N. flexuosa xyn11A produced about 1.9 g liter-1 of the xylanase in laboratory-scale fermentations. The xylanase constituted about 25% of the secreted proteins. The production of the truncated xylanase did not induce the unfolded protein response (UPR) pathway. However, the UPR was induced when the full-length N. flexuosa xyn11A with an exact fusion to the cel7A terminator was expressed. We suggest that the T. reesei folding/secretion machinery is not able to cope properly with the bacterial CBM when the mRNA of the full-length N. flexuosa xyn11A is efficiently translated.
Ramp calibration of temperature sensors (2011)

This paper describes a new approach to the calibration of temperature sensors. The traditional approach, in which the output of a device-under-test (DUT) and that of a reference sensor are compared at a few temperatures, is time consuming and, therefore, significantly increases production costs. The proposed method, in which a temperature ramp is applied to the DUT, is much faster and therefore lower cost. Moreover, unlike the conventional approach, in which calibration data is only obtained at a few temperatures, the proposed approach facilitates the characterization of the DUT over its full operating range.
Mitigating Distractions from Smartphones (2012)

A smartphone, with its versatile connectivity and incredible computational capability, can easily become an indispensable part of a user’s day. But that same user can quickly become addicted to his/her device, constantly checking
Penalty-free 10 Gbit/s single-channel co-pumped distributed Raman amplification using low RIN 14xx nm DFB pump (2002)

A penalty-free 10 Gbit/s single-channel transmission with co-pumped distributed Raman amplification using novel high power, low relative integrity noise (RIN), multimode 14xx nm distributed feedback pump is reported. No penalty from pump to signal RIN transfer was observed with co-pumped Raman gains up to 6 dB large effective area fibre (LEAF). In contrast, 0.5 dB penalty was observed with a fibre Bragg grating stabilised pump at 6 dB of Raman gain.
Silicon dual-ring modulator driven by differential signal (2014)

A silicon dual-ring modulator consisting of two serially cascaded rings with embedded PN junctions is driven by a differential signal pair. We show by simulation and experiment that the device has advantages over the single-ring modulator in terms of optical bandwidth, 3-dB modulation bandwidth and bit rate, at the expense of a 1.7-dB increase in the transmission penalty and a twofold increase of the RF power consumption. Driven by differential pseudo random binary sequence (PRBS) signals of 0.5-V peak-to-peak voltage (Vpp), the dual-ring modulator exhibits optical bandwidths of 66 pm and 40 pm at 12.5 Gb/s and 20 Gb/s, respectively. In contrast, the single-ring modulator has an optical bandwidth of 26 pm under a single-end PRBS signal of 0.5 Vpp at 12.5 Gb/s, and its eye diagram closes if the bit rate rises to 20 Gb/s.
Detoxification of ivermectin by ATP binding cassette transporter C4 and cytochrome P450 monooxygenase 6CJ1 in the human body louse, Pediculus humanus humanus (2017)

We previously observed that ivermectin-induced detoxification genes, including ATP binding cassette transporter C4 (PhABCC4) and cytochrome P450 6CJ1 (CYP6CJ1) were identified from body lice following a brief exposure to a sublethal dose of ivermectin using a non-invasive induction assay. In this current study, the functional properties of PhABCC4 and CYP6CJ1 were investigated after expression in either X. laevis oocytes or using a baculovirus expression system, respectively. Efflux of [<sup>3</sup> H]-9-(2-phosphonomethoxyethyl) adenine ([<sup>3</sup> H]-PMEA), a known ABCC4 substrate in humans, was detected from PhABCC4 cRNA-injected oocytes by liquid scintillation spectrophotometric analysis and PhABCC4 expression in oocytes was confirmed using ABC transporter inhibitors. Efflux was also determined to be ATP-dependent. Using a variety of insecticides in a competition assay, only co-injection of ivermectin and dichlorodiphenyltrichloroethane led to decreased efflux of [<sup>3</sup> H]-PMEA. PhABCC4-expressing oocytes also directly effluxed [<sup>3</sup> H]-ivermectin, which increased over time. In addition, ivermectin appeared to be oxidatively metabolized and/or sequestered, although at low levels, following functional expression of CYP6CJ1 along with cytochrome P450 reductase in Sf9 cells. Our study suggests that PhABCC4 and perhaps CYP6CJ1 are involved in the Phase III and Phase I xenobiotic metabolism of ivermectin, respectively, and may play an important role in the evolution of ivermectin resistance in lice and other insects as field selection occurs.
Successful conservative treatment of severe mucormycosis in a pedriatic patient with acute leukemia (2019)

Invasive mucormycosis is a life-threatening fungal infection in patients with severe and prolonged immunoimmunodeficiency. Radical surgery with the aim to prevent local infiltration and septic dissemination is currently considered the Gold standard of therapy. However, especially in patients with ENT involvement, this approach often results in large defects with poor functional and cosmetic outcome, thus raising the question of alternative treatment options.
Cancer Testis Antigen Vaccination Affords Long-Term Protection in a Murine Model of Ovarian Cancer (2010)

Sperm protein (Sp17) is an attractive target for ovarian cancer (OC) vaccines because of its over-expression in primary as well as in metastatic lesions, at all stages of the disease. Our studies suggest that a Sp17-based vaccine can induce an enduring defense against OC development in C57BL/6 mice with ID8 cells, following prophylactic and therapeutic treatments. This is the first time that a mouse counterpart of a cancer testis antigen (Sp17) was shown to be expressed in an OC mouse model, and that vaccination against this antigen significantly controlled tumor growth. Our study shows that the CpG-adjuvated Sp17 vaccine overcomes the issue of immunologic tolerance, the major barrier to the development of effective immunotherapy for OC. Furthermore, this study provides a better understanding of OC biology by showing that Th-17 cells activation and contemporary immunosuppressive T-reg cells inhibition is required for vaccine efficacy. Taken together, these results indicate that prophylactic and therapeutic vaccinations can induce long-standing protection against OC and delay tumor growth, suggesting that this strategy may provide additional treatments of human OC and the prevention of disease onset in women with a family history of OC.
Comparison of long-term stability of AM versus FM gyroscopes (2016)

A vibrating ring gyroscope is operated in AM and FM mode to demonstrate improved long-term stability of FM operation. Both bias and scale-factor (SF) accuracy were tested over a one-month period with devices power cycled between measurements. Bias and SF improve by more than two orders-of-magnitude from 335mdps to 2.86mdps and from 518ppm to 4.31ppm in the AM and FM modes, respectively. The temperature coefficients decrease from over 700dph/°C to less than 3dph/°C for the bias and 105ppm/°C to 1.25ppm/°C for the SF. The in-run bias improves from 19dph to less than 1.5dph. AM and FM tests use the same transducer with no additional trimming, demonstrating the fundamental advantage of FM over AM readout.
Radiology: Imaging Trauma Patients in a Deployed Setting (2018)

Medical imaging plays a critical role in the rapid diagnosis, effective triage, and management of complex poly-trauma patients. High-quality medical imaging can be accomplished successfully in a deployed or wartime setting. Due to advances in aggressive resuscitation techniques and the speed of the latest generation computed tomography scanners (64-detector and beyond), rapid trauma scans utilizing computed tomography and ultrasound imaging can routinely be performed prior to taking the patient to the operating room potentially providing the trauma team with lifesaving information. This clinical practice guideline provides an overview of the imaging modalities available in austere settings, the equipment required, and the role that each plays in triaging and diagnosis of the acutely injured poly-trauma patients.
Degenerative Myopathy of the Musculus supracoracoideus and Production Traits in Strains of Meat-Type Chickens (1984)

The incidence of degenerative myopathy of the Musculus supracoracoideus (DMS) was calculated in 23 strains comprising 1868 meat-type chickens. Twenty strains were commercial parent stocks and three strains were experimental strains from Ottawa similar to commercial broiler stocks of some 15 to 20 years ago. In the commercial strains DMS incidence ranged from 0 to 43% in males and 0 to 22% in females at ages ranging from 49 to 68 weeks. The three experimental strains did not have DMS. Data from strains with DMS were analyzed for associations between DMS and each of a number of body size and egg production traits. The DMS-positive birds had a larger breast angle (P less than .05) measured at 6 weeks of age (93 vs. 89 degrees) and a larger body weight (P less than .01) at 52 weeks (3748 vs 3594 g) than DMS-free birds. No significant relationships with other body size or egg production traits were detected.
IFITM1 targets HIV-1 latently infected cells for antibody-dependent cytolysis (2017)

HIV-1 persistence in latent reservoirs during antiretroviral therapy (ART) is the main obstacle to virus eradication. To date, there is no marker that adequately identifies latently infected CD4<sup>+</sup> T cells in vivo. Using a well-established ex vivo model, we generated latently infected CD4<sup>+</sup> T cells and identified interferon-induced transmembrane protein 1 (IFITM1), a transmembrane antiviral factor, as being overexpressed in latently infected cells. By targeting IFITM1, we showed the efficient and specific killing of a latently infected cell line and CD4<sup>+</sup> T cells from ART-suppressed patients through antibody-dependent cytolysis. We hypothesize that IFITM1 could mark natural reservoirs, identifying an immune target for killing of latently infected cells. These novel insights could be explored to develop clinical therapeutic approaches to effectively eradicate HIV-1.
French-Canadian linguistic validation of the NIH Chronic Prostatitis Symptom Index. (2005)

When applied to CPPS and control subjects, the French Canadian CPSI translation demonstrates excellent discriminant properties. Moreover, its reliability and validity characteristics confirm the qualities of the CPSI as a standard evaluative tool for men with CPPS.
Integrated predictive call admission control and resource reservation for wireless networks: an adaptive filtering approach (2012)

ABSTRACT Allocation of network resources in determining whether the connection can be established or not while maintaining stringent quality of service under desired traffic condition is the principal objective of a call admission control (CAC) algorithm. Radio resources when applied to real‐time applications may lead to over‐provisioning in wireless networks. Although CAC and resource reservation are treated independently in the literature, an attempt has been undertaken in this paper to formulate an integrated algorithm capable of providing accurate predictive CAC and resource reservation techniques with optimal resource utilization. The algorithm has been developed using the concept of adaptive filtering. The principal focus has been directed to introduce normalized least mean square adaptive filtering to estimate the source traffic characteristics. With extensive simulation results, we show the effectiveness of the proposed scheme and analyse the significance of different parameters involved in the scheme to optimize the overall radio resource management.Copyright © 2012 John Wiley &amp; Sons, Ltd.
Pressure vs. Flow Relationship for Pipe Leaks (2009)

Leakage can be reduced by reducing pressure in a water distribution system. The magnitude of that reduction depends on the equation used to predict leakage. A common equation is to use a power function where the flow is estimated from the pressure terms raised to an exponent. There are a wide range of values reported in the literature for that exponent. Laboratory experiments were performed in which flow and pressure were measured for a wide range of leak shapes and sizes. The median value of the exponent was 0.54 which is lower than most reported but close to the theoretical value for an orifice. The high values in the literature can be explained in some cases as due to the method for estimating leak reduction. This is illustrated using a model of a small zone.
π-Facial selectivity in the Diels-Alder reactions of the 1,3-cyclohexadienes bearing allylic oxygen substitution (1992)

The Diels-Alder reaction of cis-3,5-cyclohexadiene-1,2-diol (121) and a number of its acyclic derivatives, cis-1,2-diacetoxy-3,5-cyclohexadiene (109), cis-1,2-bis- (trimethylsiloxy)-3I5-cyclohexadiene(122)andcis-1,2-dimethoxy-3l5-cyclohexadiene(124) were found to add N-phenylmaleimide in a kinetically controlled manner to yield adducts that arose mainly by attachment of the dienophile to the face of the diene syn to the oxygen atoms. Some cyclic derivatives of 121, cis-3a,7a-dihydro-2,2-dimethyl -1,3- benzodioxole (114) ,cis-3a,7a-dihydro-2,2-dimethyl-1,3,2-ben2odioxasilole (123) and cis-2- ethyl-3a,7a-dihydro-2,1,3-benzoboradioxoie (125), afforded nearly equal quantities of both syn and anti adducts. Benzylidene-protected derivatives (2α,3aβ,7aβ)-3a,7a-dihydro-2- phenyl-1,3-benzodioxole (132) and (2α,3aα,7aβ)-3a,7a-dihydro-2-phenyl-1,3-benzodioxole (133) also reacted with N-phenylmaleimide, but they gave predominantly anti-addition products. -- 1,3,5-Cyclohexatriene-1,2-oxide (142) and its more substituted derivatives, 1,2- dimethyl-1,3,5-cyclohexatriene-1,2-oxide (148a) and 10-oxatricyclo[4.3.1.0]deca-2,4- diene (148b) reacted also with N-phenylmaleimide to give only products that resulted from addition of the addends to the face of the diene anti to the epoxide oxygen. -- The results obtained have been discussed in the context of the many theories that attempt to define the controlling factors involved in determining (syn/anti) π-facial selectivity of plane-nonsymmetric dienes bearing an allylic heteroatom. Steric effects and stereoelectronic effects are invoked in this study to rationalize the results we report here. In particular, our contrasting results with the diol-derived dienes and the benzene oxides are consistent with syn-addition being determined by the presence of a favorable secondary orbital interaction between the LUMO of the dienophile and the components of the highest occupied molecular orbitals that reside on the allylic oxygens of the dienes.
Atmosphere aerosol modeling by GEOS-Chem for the “Aerosol-UA” space project validation (2017)

We used a global chemical transport model GEOS-Chem to compute monthly mean fine, coarse, and total aerosol volume concentration for Minsk and Kyiv in the period from 2010 to 2015. We compared results of the model simulation with sun-photometer observations at the ground-based AERONET network sites. We obtained that the aerosol volume concentrations retrieved from observations are in reasonably good agreement with model-simulated ones. However, the agreement is not good enough for the validation of the satellite measurements in the future space mission Aerosol-UA by the GEOS-Chem model simulation. To improve the accuracy of estimating the spatial-temporal distribution of the aerosol volume concentration we decided to apply the optimal interpolation method for assimilating AERONET data in GEOS-Chem model. The temporal correlation function for fine aerosol volume concentration is obtained on the basis of measurements at AERONET Minsk site over the 2002-2015 period and Kyiv site over the 2008-2015 period. We describe the analyzed values of fine aerosol volume concentration at all temporal grid points over the period of 2002 to 2015 for Minsk site and of 2008 to 2015 for Kyiv site, which were determined on the basis of the optimal interpolation method. We propose to use the optimal averaging method for AERONET data on the basis of the temporal optimization interpolation method.
Quasi-simultaneous coherent Raman imaging of components in the fingerprint, silent and high-wavenumber region with a portable all-fiber light source (Conference Presentation) (2020)

We present high-speed multicolor coherent Raman imaging enabled by a novel all-fiber light source. A high tuning speed of < 5 ms per arbitrary wavelength step (700 - 3530 wavenumbers) allows to switch the wavelength in a frame-by-frame manner when imaging with up to 100 frames/s. The pump (up to 200 mW) and Stokes pulses (> 400 mW) exhibit equal durations of 7 ps. The compact and environmentally stable system is predestined for fast multicolor assessments of medical or rapidly evolving samples with high chemical specificity.
Chronic Baroreflex Activation Effects on Sympathetic Nerve Traffic, Baroreflex Function, and Cardiac Haemodynamics in Heart Failure: A Proof-of-Concept Study (2014)

BAT was safe and provided chronic improvement in MSNA and clinical variables. Based on present understanding of HF pathophysiology, these results suggest that BAT may improve outcome in HF by modulating autonomic balance. Prospective, randomized trials to test the hypothesis are warranted.
Polymerization and crystallization behavior of a LaRC‐TPI powder (1988)

Abstract The crystallization and polymerization behavior of a crystalline LaRC‐TPI powder at elevated temperatures have been studied. The characterization methods include differential scanning calorimetry and the measurements of inherent viscosity, η inh , and viscoelastic properties of the fully imidized samples. The as‐Received material possesses an initial crystal melting peak temperature of 272°C. For the material annealed at temperatures below 320°C, a semicrystalline polymer can be obtained. On the other hand, a purely amorphous structure is realized in the samples annealed at temperatures above 320°C. Isothermal crystallization kinetics are studied by means of the simple. Avrami equation. The likely nucleation path is attributed as an intermolecular oligomer nucleation type. The isothermal polymerization behavior is quantified by inherent viscosity measurements. A single cure is found to correlate values of T g and η inh for samples experiencing different thermal histories. Fully imidized amorphous and semicrystalline LaRC‐TPI samples are successfully molded based on the information obtained from thermal analyses. The viscoelastic properties of these samples are also reported.
[Another case of Hantaan virus infection in The Netherlands]. (1989)

In a serological survey among Dutch patients suspected of leptospirosis, using a recently developed enzyme-linked immunosorbent assay, a patient was traced with a high antibody titre to Hantaan virus. No anti-leptospira antibodies were detected in this 27-year-old man. Shortly before he had been admitted to the hospital with progressive dyspnoea and coughing, accompanied with high fever. An interstitial pneumonia was diagnosed. He subsequently developed a progressive renal failure with proteinuria and polyuria. Later a liver failure accompanied with thrombocytopenia, anaemia and coagulation disturbances occurred. Before an aetiological diagnosis was made, the patient was treated with erythromycin. The patient eventually recovered completely. Based on the clinical symptoms and the positive serology, it was concluded that the disease diagnosed had probably been caused by a Hantaan virus infection. The diagnostic value of Hantaan virus serology in patients with similar symptoms is stressed.
Transcriptional responses ofSaccharomyces cerevisiaeto preferred and nonpreferred nitrogen sources in glucose-limited chemostat cultures (2007)

Aerobic, glucose-limited chemostat cultures of Saccharomyces cerevisiae grown with six different nitrogen sources were subjected to transcriptome analysis. The use of chemostats enabled an analysis of nitrogen-source-dependent transcriptional regulation at a fixed specific growth rate. A selection of preferred (ammonium and asparagine) and nonpreferred (leucine, phenylalanine, methionine and proline) nitrogen sources was investigated. For each nitrogen source, distinct sets of genes were induced or repressed relative to the other five nitrogen sources. In total, 131 such 'signature transcripts' were identified in this study. In addition to signature transcripts, genes were identified that showed a transcriptional coresponse to two or more of the six nitrogen sources. For example, 33 genes were transcriptionally upregulated in leucine-grown, phenylalanine-grown and methionine-grown cultures; this was partly attributed to the involvement of common enzymes in the dissimilation of these amino acids. In addition to specific transcriptional responses elicited by individual nitrogen sources, their impact on global regulatory mechanisms such as nitrogen catabolite repression (NCR) were monitored. NCR-sensitive gene expression in the chemostat cultures showed that ammonium and asparagine were 'rich' nitrogen sources. By this criterion, leucine, proline and methionine were 'poor' nitrogen sources, and phenylalanine showed an 'intermediate' NCR response.
Skeletal myogenesis on highly orientated microfibrous polyesterurethane scaffolds (2007)

Skeletal myogenesis is a complex process, which is known to be intimately depending on an optimal outside-in substrate-cell signaling. Current attempts to reproduce skeletal muscle tissue in vitro using traditional scaffolds mainly suffer from poor directionality of the myofibers, resulting in an ineffective vectorial power generation. In this study, we aimed at investigating skeletal myogenesis on novel biodegradable microfibrous scaffolds made of DegraPol, a block polyesterurethane previously demonstrated to be suitable for this application. DegraPol was processed by electrospinning in the form of highly orientated ("O") and nonorientated ("N/O") microfibrous meshes and by solvent-casting in the form of nonporous films ("F"). The effect of the fiber orientation at the scaffold surface was evaluated by investigating C2C12 and L6 proliferation (via SEM analysis and alamarBlue test) and differentiation (via RT-PCR analysis and MHC immunostaining). We demonstrated that highly orientated elastomeric microfibrous DegraPol scaffolds enable skeletal myogenesis in vitro by aiding in (a) myoblast adhesion, (b) myotube alignment, and (c) noncoplanar arrangement of cells, by providing the necessary directional cues along with architectural and mechanical support.