gebhart/patrepeval huggingface dataset:
Synthesis of a deuterated probe for the confocal Raman microscopy imaging of squalenoyl nanomedicines (2016)

The synthesis of ω-di-(trideuteromethyl)-trisnorsqualenic acid has been achieved from natural squalene. The synthesis features the use of a Shapiro reaction of acetone-d 6 trisylhydrazone as a key step to implement the terminal isopropylidene-d 6 moiety. The obtained squalenic acid-d 6 has been coupled to gemcitabine to provide the deuterated analogue of squalenoyl gemcitabine, a powerful anticancer agent endowed with self-assembling properties. The Raman spectra of both deuterated and non-deuterated squalenoyl gemcitabine nanoparticles displayed significant Raman scattering signals. They revealed no differences except from the deuterium peak patterns in the silent spectral region of cells. This paves the way for label-free intracellular trafficking studies of squalenoyl nanomedicines.
A Hybrid ToF Image Sensor for Long-Range 3D Depth Measurement Under High Ambient Light Conditions (2023)

A new indirect time of flight (iToF) sensor realizing long-range measurement of 30 m has been demonstrated by a hybrid ToF (hToF) operation, which uses multiple time windows (TWs) prepared by multi-tap pixels and range-shifted subframes. The VGA-resolution hToF image sensor with 4-tap and 1-drain pixels, fabricated by the BSI process, can measure a depth of up to 30 m for indoor operation and 20 m for outdoor operation under high ambient light of 100 klux. The new hToF operation with overlapped TWs between subframes for mitigating an issue on the motion artifact is implemented. The sensor works at 120 frames/s for a single subframe operation. Interference between multiple ToF cameras in IoT systems is suppressed by a technique of emission cycle-time changing.
Use of P-Element-Mediated Transformation to Identify the Molecular Basis of Naturally Occurring Variants Affecting Adh Expression in Drosophila melanogaster (1987)

The purpose of the work reported here is to identify the molecular basis of the difference in level of expression between the polymorphic Slow and Fast alcohol dehydrogenase (Adh) alleles in Drosophila melanogaster. Previous studies have shown that Fast lines typically have a two- to threefold higher activity level than Slow lines and they also have a substantially higher level of ADH-protein (estimated immunologically). The results of a restriction fragment length polymorphism study in relation to ADH activity variation had previously suggested that the difference in Adh expression between allozymes might not be due entirely to the amino acid replacement substitution, but could be due in part to linkage disequilibrium with a regulatory site polymorphism. Here we describe an approach that makes use of P-element-mediated transformation in order to identify the nucleotide substitution(s) responsible for this difference in ADH level. This approach consists of generating recombinants in vitro between Adh region clones derived from a typical Slow/Fast pair of alleles and then testing for the effects of particular restriction fragments on expression in vivo by transformation. Using this approach, the effect on both ADH activity and ADH-protein level clearly maps to a 2.3-kb restriction fragment that includes all of the Adh coding sequence and some intron and 3' flanking sequence, but excludes all of the 5' flanking sequence of the distal (adult) transcriptional unit. Comparison of Kreitman's DNA sequences for this fragment from several Slow and Fast alleles showing the typical difference in activity level shows that only three nucleotide substitutions distinguish all Fast from all Slow alleles. Thus, it is likely that one or more of these substitutions causes the major difference in Adh expression between allozymic classes. One of these substitutions is, of course, the Slow/Fast amino acid replacement substitution (at 1490) while the other two are nearby third position silent substitutions (at 1443 and 1527). A quantitative analysis of variation among transformant stocks shows that the P-element transformation approach can be used to localize even relatively small effects on gene expression (on the order of 20%).
Rectifying transformations that minimize resampling effects (2005)

Image rectification is the process of warping a pair of stereo images in order to align the epipolar lines with the scan-lines of the images. Once a pair of images is rectified, stereo matching can be implemented in an efficient manner. Given the epipolar geometry, it is straightforward to define a rectifying transformation, however, many transformations will lead to unwanted image distortions. In this paper, we present a novel method for stereo rectification that determines the transformation that minimizes the effects of resampling that can impede stereo matching. The effects we seek to minimize are the loss of pixels due to under-sampling and the creation of new pixels due to over-sampling. To minimize these effects we parameterize the family of rectification transformations and solve for the one that minimizes the change in local area integrated over the area of the images.
Bioethical aspects of end-of-life care (2008)

Managing end-of-life care can be difficult because of the particular nature of intensive care support, which can separate the biological and the biographical aspects of life. Artificial organ support can temporarily delay death but, at the same time, may fail to restore a quality of life that the patient judges acceptable. For this reason, two concepts must be considered: that the mission of the healthcare system should be to care for patients according to their interests and wishes and that quality of care is related above all to the careful commitment of healthcare workers to the patient's best interests. Keeping these concepts in mind, the rule of the five Cs (competence, collegiality, communication, continuity of care and compassion) might be helpful in the management of end-of-life care. Unfortunately, neither the rule of the five Cs nor the careful use of moral principles in order to promote the patients' dignity can assure a universally acceptable decision. A reasonable level of 'moral certainty', however, might be achieved using a deliberative approach, which provides for the inclusion of all the different subjects involved in the decision-making process (patient, family, doctors, nurses and other carers), in order to reach the best possible decision in a specific situation.
Interaction of Cyclin-Dependent Kinase 12/CrkRS with Cyclin K1 Is Required for the Phosphorylation of the C-Terminal Domain of RNA Polymerase II (2012)

CrkRS (Cdc2-related kinase, Arg/Ser), or cyclin-dependent kinase 12 (CKD12), is a serine/threonine kinase believed to coordinate transcription and RNA splicing. While CDK12/CrkRS complexes were known to phosphorylate the C-terminal domain (CTD) of RNA polymerase II (RNA Pol II), the cyclin regulating this activity was not known. Using immunoprecipitation and mass spectrometry, we identified a 65-kDa isoform of cyclin K (cyclin K1) in endogenous CDK12/CrkRS protein complexes. We show that cyclin K1 complexes isolated from mammalian cells contain CDK12/CrkRS but do not contain CDK9, a presumed partner of cyclin K. Analysis of extensive RNA-Seq data shows that the 65-kDa cyclin K1 isoform is the predominantly expressed form across numerous tissue types. We also demonstrate that CDK12/CrkRS is dependent on cyclin K1 for its kinase activity and that small interfering RNA (siRNA) knockdown of CDK12/CrkRS or cyclin K1 has similar effects on the expression of a luciferase reporter gene. Our data suggest that cyclin K1 is the primary cyclin partner for CDK12/CrkRS and that cyclin K1 is required to activate CDK12/CrkRS to phosphorylate the CTD of RNA Pol II. These properties are consistent with a role of CDK12/CrkRS in regulating gene expression through phosphorylation of RNA Pol II.
Assessing the scalability of dynamic field gradient focusing by linear modeling (2008)

Dynamic field gradient focusing (DFGF) separates and concentrates proteins in native buffers, where proteins are most soluble, using a computer-controlled electric field gradient which lets the operator adjust the pace and resolution of the separation in real-time. The work in this paper assessed whether DFGF could be scaled up from microgram analytical-scale protein loads to milligram preparative-scale loads. Linear modeling of the electric potential, protein transport, and heat transfer simulated the performance of a preparative-scale DFGF instrument. The electric potential model showed where the electrodes should be placed to optimize the shape and strength of the electric field gradient. Results from the protein transport model suggested that in 10 min the device should separate 10 mg each of two proteins whose electrophoretic mobilities differ by 5 x. Proteins with electrophoretic mobilities differing by only 5% should separate in 3 h. The heat transfer model showed that the preparative DFGF design could dissipate 1 kW of Joule heat while keeping the separation chamber at 25 degrees C. Model results pointed to DFGF successfully scaling up by 1000 x using the proposed instrument design.
Robust adaptive beamformers with linear matrix inequality constraints (2008)

In this paper, a novel robust adaptive beamformers with linear matrix inequality (LMI) constraints on magnitude response is proposed. The recently proposed robust adaptive beamformer has a drawback that some of the constraints are semi-infinite. Although the sampling technique provides a good approximation of the optimization problem, the cost is the heavy computation load. In order to overcome this problem, new LMI constraints are derived in this paper to replace the semi- infinite constraints so that the sampling is avoid and an exact solution can be obtained.
Label-Free Biosensing with Lipid-Functionalized Gold Nanorods (2011)

The fabrication of a label-free mass spectrometry and optical detection-based biosensor platform for the detection of low-abundance lipophilic analytes in complex mixtures is described. The biosensor consists of a lipid layer partially tethered to the surface of a gold nanorod. The effectiveness of the biosensor is demonstrated for the label-free detection of a lipophilic drug in aqueous solution and a lipopeptide in serum.
Three-dimensional analysis of dual track complementary type of heads for perpendicular magnetic recording (1998)

The reproduction flux distributions of the dual track complementary (DTC) type of heads for perpendicular magnetic recording were calculated using a 3-D finite element method and compared with those of the single pole type (SPT) of heads. The calculated reproduction flux hardly changed along the head pole, which indicates that the flux sensitive detector can be placed far from the air bearing slider surface in the DTC head system. As a result, the DTC type of recording heads may be a candidate for heads for perpendicular magnetic recording systems.
Technical Note: Further development of a resolution modification routine for the simulation of the modulation transfer function of digital x‐ray detectors (2011)

The developed modification routine provides the means to simulate the spatial resolution of digital x-ray detectors under a wider range of conditions.
Discovery of a Novel, Monocationic, Small-Molecule Inhibitor of Scrapie Prion Accumulation in Cultured Sheep Microglia and Rov Cells (2012)

Prion diseases, including sheep scrapie, are neurodegenerative diseases with the fundamental pathogenesis involving conversion of normal cellular prion protein (PrP(C)) to disease-associated prion protein (PrP(Sc)). Chemical inhibition of prion accumulation is widely investigated, often using rodent-adapted prion cell culture models. Using a PrP(Sc)-specific ELISA we discovered a monocationic phenyl-furan-benzimidazole (DB772), which has previously demonstrated anti-pestiviral activity and represents a chemical category previously untested for anti-prion activity, that inhibited PrP(Sc) accumulation and prion infectivity in primary sheep microglial cell cultures (PRNP 136VV/154RR/171QQ) and Rov9 cultures (VRQ-ovinized RK13 cells). We investigated potential mechanisms of this anti-prion activity by evaluating PrP(C) expression with quantitative RT-PCR and PrP ELISA, comparing the concentration-dependent anti-prion and anti-pestiviral effects of DB772, and determining the selectivity index. Results demonstrate at least an approximate two-log inhibition of PrP(Sc) accumulation in the two cell systems and confirmed that the inhibition of PrP(Sc) accumulation correlates with inhibition of prion infectivity. PRNP transcripts and total PrP protein concentrations within cell lysates were not decreased; thus, decreased PrP(C) expression is not the mechanism of PrP(Sc) inhibition. PrP(Sc) accumulation was multiple logs more resistant than pestivirus to DB772, suggesting that the anti-PrP(Sc) activity was independent of anti-pestivirus activity. The anti-PrP(Sc) selectivity index in cell culture was approximately 4.6 in microglia and 5.5 in Rov9 cells. The results describe a new chemical category that inhibits ovine PrP(Sc) accumulation in primary sheep microglia and Rov9 cells, and can be used for future studies into the treatment and mechanism of prion diseases.
A Simple and Efficient Ozone Generator (1999)

We report on the construction of an inexpensive, efficient, and compact ozonolysis apparatus, suitable for use in oxidation chemistry, which is a convenient alternative to the more expensive generators currently on the market. The details, operating parameters, and application of the ozone generator to the oxidation of methylallyl oxazolidione are also presented.
Dual Effects of Statins on Aβ Metabolism: Upregulation of the Degradation of APP-CTF and Aβ Clearance (2012)

Statins might prevent the onset of AD through reduced Aβ production by enhancement of APP-CTF degradation and/or upregulation of Aβ clearance. We also showed that promotion of APP-CTF degradation and upregulation of Aβ clearance could be modified by a drug, suggesting possible mechanistic targets for disease-modifying drugs.
DNA‐Coated AFM Cantilevers for the Investigation of Cell Adhesion and the Patterning of Live Cells (2008)

Get a grip: Measurement of receptor adhesion strength requires the precise manipulation of single cells on a contact surface. To attach live cells to a moveable probe, DNA sequences complementary to strands displayed on the plasma membrane are introduced onto AFM cantilevers (see picture, bp=base pairs). The strength of the resulting linkages can be tuned by varying the length of DNA strands, allowing for controlled transport of the cells. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
The Monomeric G-Proteins Rac1 and/or Cdc42 Are Required for the Inhibition of Voltage-Dependent Calcium Current by Bradykinin (1997)

Although regulation of voltage-dependent calcium current (ICa,V) by neurotransmitters is a ubiquitous mechanism among nerve cells, the signaling pathways involved are not well understood. We have determined previously that in a neuroblastoma-glioma hybrid cell line (NG108-15), the heterotrimeric G-protein G13 mediates the inhibition of ICa,V produced by bradykinin (BK) via an unknown mechanism. Various reports indicate that G13 can couple to RhoA, Rac1, and Cdc42, which are closely related members of the Rho family of monomeric G-proteins. We have investigated their role as signaling intermediates in the pathway used by BK to inhibit ICa,V. Using immunoblot analysis and the PCR, we found evidence that RhoA, Rac1, and Cdc42 all are expressed in NG108-15 cells. Intracellularly perfused recombinant Rho-GDI (an inhibitor of guanine nucleotide exchange specific for the Rho family) attenuated the inhibition of ICa,V by BK. These findings indicate that activation of RhoA, Rac1, or Cdc42 may be required for the response to BK. To determine whether any of these monomeric G-proteins mediate the response to BK, we have intracellularly applied blocking antibodies specific for each of the candidate proteins. Only the anti-Rac1 antibody blocked the response to BK. In parallel experiments, peptides corresponding to the C-terminal regions of Rac1 and Cdc42 blocked the same response. These data indicate a novel functional contribution of Rac1 and possibly also of Cdc42 to the inhibition of ICa,V by neurotransmitters.
THE DEVELOPMENT METHOD OF APPROXIMATION THE OBJECTIVE FUNCTION TO SOLVE FRACTIONAL LINEAR FRACTIONAL PROBLEMS. (2009)

Approximation of Fractional objective function one of methods to solve Fractional linear programming problems(F.L.P.P.) using steps and Approximation of objective function. In this paper we development of this method (new methods to solve To Solve FLP
Health literacy is a barrier to shared decision making in early prostate cancer (PCA) among African American (AA) men. (2019)

84 Background: ASCO, AUA, ASTRO and SUO endorse shared decision making for men with localized PCa. We explored treatment decisions among providers and their AA patients (pts) in a prospective cohort study at Grady Memorial Hospital and the Atlanta Veterans Administration Hospital. Methods: Following their visit, 18 providers documented the PCa treatment options they had discussed with 124 newly diagnosed, early-stage, African American PCa pts. At a subsequent visit, prior to choosing their cancer treatment, pts were asked to name the options they had discussed with their provider. Demographics were collected. Health literacy was measured using the Rapid Estimate of Adult Literacy in Medicine (REALM). Numeracy, comprehension of common PCa terms, and anatomic knowledge were assessed using published methods (Kilbridge K, et al. J Clin Oncol 27:2015-2021, 2009). Chi-square, t-tests and multivariate logistic regression were used to identify variables associated with correct understanding of treatment choices. Results: Just 23.4% of pts correctly understood their treatment options. In univariate analysis, only health literacy was statistically significantly associated with comprehension of PCa treatment options (p &lt; 0.05). In a multivariate logistic model adjusting for age, education, income, numeracy, comprehension of common PCa terms, and anatomic knowledge; health literacy remained the only significant predictor of pts’ comprehension of their treatment choices (OR 3.8, 95% CI 1.2-11.9, p = 0.021). Even among the 49 pts with the highest level of health literacy, only 34.7% correctly understood their cancer treatment options (compared to 16.0% among low literacy patients). Conclusions: Successful shared decision making requires pts to understand their treatment choices. Information presented by healthcare providers may be overwhelming for newly diagnosed pts, particularly those with lower health literacy. Our study suggests that even pts with the highest level of health literacy may need additional support to understand their PCa treatment options.
The Stereochemistry of Complex Inorganic Compounds. XVII. The Stereochemistry of Hexadentate Ethylenediaminetetraacetic Acid Complexes (1953)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Stereochemistry of Complex Inorganic Compounds. XVII. The Stereochemistry of Hexadentate Ethylenediaminetetraacetic Acid ComplexesDaryle H. Busch and John C. Bailar Jr.Cite this: J. Am. Chem. Soc. 1953, 75, 18, 4574–4575Publication Date (Print):September 1, 1953Publication History Published online1 May 2002Published inissue 1 September 1953https://pubs.acs.org/doi/10.1021/ja01114a054https://doi.org/10.1021/ja01114a054research-articleACS PublicationsRequest reuse permissionsArticle Views328Altmetric-Citations135LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Evolution of the Borehole Seismic Offer for Velocity Prediction ahead of the Bit (2017)

This paper provides an overview of the knowledge and experience accumulated over the years in the use of borehole seismic data to produce reliable predictive velocity profiles ahead of the deepest vertical seismic profile (VSP) level recorded. The historical acoustic and 1D (ZVSP) approach had serious limitations. Software techniques developed after 2000 around 2D walkaway geometry led to significant progress and successful projects, enabling the estimation of look-ahead elastic parameters with increasing accuracy. These parameters, which are the input to empirical transforms to pore pressure, are particularly critical for geomechanics engineers (and therefore drillers), to detect anomalies and prevent drilling hazards. The recent improvement of the full waveform inversion (FWI) techniques and the wider use of reflection seismic tomography algorithms open a new era of progress on the topic. The status of the development and evaluation of these techniques is described.
Recrystallization Mechanism for Solid Phase Growth of Poly-Si Films on Quartz Substrates (1988)

The recrystallization mechanism for solid-phase growth of poly-Si films amorphized by ion implantation on quartz substrates is clarified on the basis of an experimental finding obtained through the TEM observation. It is found that the {110} textured nucleation occurs. The preferential growth in the &lt;112 &gt; directions along twin boundaries then leads to the formation of the dendritic structure.