gebhart/patrepeval huggingface dataset:
Viable/non-viable cell assay using electrokinetic deterministic lateral displacement (2019)

To evaluate the effect of antibiotic drugs, it is important that the cells that are killed can be sorted from the cells that are resistant to the antibiotics. Here we report a simple label-free method for the continuous separation of viable/non-viable cells using Electrokinetic Deterministic Lateral Displacement. In general, this opens up for continuous sorting of a population of cells that is homogeneous in size but heterogeneous in surface charge or dielectric properties, properties that change as a function of viability.
Electrical Properties (2011)

This chapter contains sections titled: Overview Mixed Composites Impregnated/Infused Composites Composites with Electrically Conducting Polymers Challenges References
P2‐085: THE ASSOCIATION BETWEEN MEASURED BLOOD PRESSURE AND INCIDENT DEMENTIA IN THE OLDEST OLD: THE 90+ STUDY (2014)

Midlife hypertension has been identified as a risk factor for dementia in later life. However, this association is less clear in the oldest-old, with some suggesting that hypertension may be protective in advanced age. We sought to evaluate the association between measured blood pressure (BP) and incident dementia in a cohort of individuals aged 90 and older after accounting for antihypertensive medication intake. A total of 625 participants without dementia from The 90+ Study (age range 90-103) were evaluated at the baseline examination, which included cognitive and neurological assessments, sitting BP measurement, and assessment of medications. Participants' cognitive status was re-evaluated every six months for up to 10 years (range 1 month-10 years, mean 2.8 years, standard deviation 2.0). BP readings (in mmHg) were classified as non-hypertensive (<120 systolic, <80 diastolic), pre-hypertensive (120-139 systolic or 80-89 diastolic), stage-1 hypertensive (140-159 systolic or 90-99 diastolic), and stage-2 hypertensive (160+ systolic or 100+ diastolic). Cox regression models, with age as the time scale, were used to evaluate survival until dementia diagnosis (using DSM-IV criteria). Models were adjusted for education and a dichotomous indicator for concurrent use of any antihypertensive medication. At follow up examinations, 259 individuals were identified with dementia. 73.5% of participants reported current intake of anti-hypertensive medications. Participants with pre-hypertension had a decreased, although non significant, risk for dementia (Table). In a trend of decreasing risks, individuals with stage-1 hypertension had an even lower risk and individuals with stage-2 hypertension had the lowest risk of dementia (0.57, 95% CI 0.33-1.00, p =0.0511). A test for trend showed a significant protective effect (χ 2, p =0.043) with increasing levels of hypertension. Current use of any anti-hypertensive medications was not related to dementia risk (Hazard Ratio=0.96, p =0.77). Contrary to studies showing that hypertension is a risk factor for dementia when present at younger ages, it appears to be protective in the oldest-old. The effect appears to be independent of antihypertensive medications and a dose-response is indicated. Our results suggest that, in relation to cognitive health, the current standard of care for hypertension may not be beneficial in the oldest-old.
Carbide-Derived Carbons (2017)

The synthesis of carbon can be arranged through many different techniques, such as chemical vapor deposition (CVD), including plasma enhanced CVD, low pressure CVD, laser ablation and arc discharge, hydrothermal synthesis, electrolysis, and various other techniques. The development of novel methods to synthesize carbon structures and to allow for strict control of the resulting structure is of key importance for the advancement of technology and for providing scientists with new means to study various phenomena. Carbide-derived carbon (CDC) has the ability to create a very uniform and controlled pore structure with the appropriate processing conditions. The porosity created within CDC is a function of many variables, including processing parameters, initial carbide chemistry and porosity, and the post-halogen treatment. The gas mixtures have been shown to affect the type of carbon forming during chlorine treatment and affect the resulting pore structure.
CTD2 Dashboard: a searchable web interface to connect validated results from the Cancer Target Discovery and Development Network (2017)

https://ctd2-dashboard.nci.nih.gov/.
Compliant microgripper with parallel straight-line jaw trajectory for nanostructure manipulation (2011)

Compliant gripping mechanisms are particularly suited for precision manipulation of micro- and nanoscale objects by virtue of having zero backlash, no parasitic columbic friction effects,
Therapy for chemopretreated metastatic urothelial cancer (mUC) with the antibody-drug conjugate (ADC) sacituzumab govitecan (IMMU-132). (2017)

327 Background: Patients (pts) with mUC pre-treated with platinum-containing chemotherapy have limited therapeutic options, with checkpoint-inhibitor immunotherapy (IO) responses in a minority of pts. We present an interim update of the safety and activity of sacituzumab govitecan (IMMU-132), an anti-Trop-2-SN-38 ADC, as therapy for chemotherapy-pretreated mUC pts (ClinicalTrials.gov, NCT01631552). Methods: We enrolled 32 pts with mUC and ECOG PS 0-1 who failed ≥ 1 prior standard therapy (median=3; range, 1-5). IMMU-132 was administered at 8 or 10 mg/kg on days 1 and 8 every 21 days, continued until disease progression (PD) or unacceptable toxicity. Response-evaluable pts received ≥ 2 doses, and had ≥ 1 post-baseline response assessment. Results: Twenty-five pts [median age 68 yrs (range: 50-91), 24 males] are currently assessable for safety and response; 23 had prior platinum-containing therapy; 46% had &gt;2 prior therapies; 4 also had IO agents. Sites of metastases included liver (N=4; 16%), lungs (N=7; 28%), bone (N=4; 16%), and lymph nodes (N=16; 64%). Pts received a median of 7 cycles (range, 2-23) of IMMU-132. ORR was 36% (9/25) [1 complete (CR) and 8 partial responses (PR)]; 44% (11/25) had stable disease (SD). Further, pts with 1 line of prior chemotherapy had an ORR of 53.8% (7/13), and 16.7% for those with 2 to 5 prior therapy lines. Median PFS for all patients is 7.2 mos (95% CI, 4.9-10.7); median survival is not reached yet. Of the 4 pts with progression after prior IO, there were 1 PR and 2 SDs with IMMU-132. Duration of response for CR/PR pts is currently 5.1 mos (95% CI, 4.1-12.9) and 10/11 pts (5 with ≥ 20% tumor reduction) had stable disease &gt; 4 mos. Grade 4 neutropenia (16%) lasted &lt; 7 days, and non-hematological grade 3 AEs included fatigue (12%) and hypophosphatemia (8%). No treatment-related deaths were observed. Analysis of Trop-2 expression revealed 1+ to 3+ positive staining in 95% of 19 archival patient specimens. Conclusions: With an ORR of 36% and a median PFS of 7.2 months in a heavily pretreated population, these interim results suggest that IMMU-132 is a promising agent as 2 nd line or later therapy for platinum- or IO- pretreated mUC pts. Clinical trial information: NCT01631552.
Automated 3D Mosaicing and Scan Trajectories for Surveillance of Bladder Cancer (2011)

While cystoscopic surveillance, which uses a type of endoscope used by urologists to view the interior surface of patient’s bladder, is regarded as the “gold standard” for bladder cancer detection, it remains imperfect. Physicians advance a rigid or flexible scope through a patient’s urethra and into his/her bladder, manually manipulating the probe in order to view the entire inner surface of the bladder. Thus, the completeness of cystoscopic examinations remains completely dependent on the examining physician. We propose a few scanning trajectories, which can be potentially adopted in the mechatronics approach to minimize operator errors. An automated image mosaicing software, which would afford 3D reconstruction of the bladder for more efficient surveillance, is proposed to achieve a high resolution and comprehensive model of the bladder. The software adequately reconstructs the internal surface of the virtual model under all three scan trajectories as a proof-of-concept.
Integrin alpha-v/beta3 expression in equine lungs and jejunum. (2020)

Integrin alpha-v/beta3 (αvβ3) recognizes arginine-glycine-aspartic acid (RGD) sequences and has important functions in cell adhesion, signaling, and survival. However, the expression of integrin αvβ3 in the equine lungs and jejunum is not well understood. The objective of this study was to explore the hitherto unknown expression of integrin αvβ3 in the lungs and jejuna of the horse using light and electron immunocytochemistry. Immunohistochemistry showed integrin αvβ3 on the epithelium, the immune cells in Peyer's patches, the smooth muscle, and the endothelium of equine jejuna. In equine lungs, we recognized integrin αvβ3 on the endothelium of blood vessels, the alveolar septa, the bronchial lymph nodes, and the cartilages, although the expression of integrin αvβ3 was weak on the epithelium of bronchioles. In conclusion, these are the first data to show the expression of integrin αvβ3 in equine lungs and jejuna.
Laparoscopic Ureterocalicostomy: Development of a Technique Simplified by Application of Nitinol Clips and a Wet Monopolar Electrosurgery Device (2005)

No AccessJournal of UrologyUrological Survey: Urolithiasis/Endourology: Urolithiasis, Endourology and Laparoscopy1 Nov 2005Laparoscopic Ureterocalicostomy: Development of a Technique Simplified by Application of Nitinol Clips and a Wet Monopolar Electrosurgery Device Ralph V. ClaymanM.D. Ralph V. ClaymanRalph V. Clayman More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)68810-4AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "Laparoscopic Ureterocalicostomy: Development of a Technique Simplified by Application of Nitinol Clips and a Wet Monopolar Electrosurgery Device." The Journal of Urology, 174(5), pp. 1849–1850 © 2005 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 174Issue 5November 2005Page: 1849-1850 Advertisement Copyright & Permissions© 2005 by American Urological Association, Inc.MetricsAuthor Information Ralph V. Clayman More articles by this author Expand All Advertisement Loading ...
Design of HTML pages to increase their accessibility to users with disabilities (1996)

article Free Access Share on Design of HTML pages to increase their accessibility to users with disabilities: strategies for today and tomorrow Authors: Gregg C. Vanderheiden Neal Ewers, Trace R & D Center, University of Wisconsin - Madison Neal Ewers, Trace R & D Center, University of Wisconsin - MadisonView Profile , Wendy A. Chisholm Neal Ewers, Trace R & D Center, University of Wisconsin - Madison Neal Ewers, Trace R & D Center, University of Wisconsin - MadisonView Profile Authors Info & Claims ACM SIGWEB NewsletterVolume 5Issue 1Feb. 1996 pp 9–20https://doi.org/10.1145/232782.232786Online:01 February 1996Publication History 5citation103DownloadsMetricsTotal Citations5Total Downloads103Last 12 Months3Last 6 weeks1 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
THE ‘GASTEIN’ SYMPHONY RECONSIDERED (1959)

THE ‘GASTEIN’ SYMPHONY RECONSIDERED Get access JOHN REED JOHN REED Search for other works by this author on: Oxford Academic Google Scholar Music and Letters, Volume XL, Issue 10, October 1959, Pages 341–349, https://doi.org/10.1093/ml/XL.10.341 Published: 01 October 1959
Receiver Architectures and Design Tradeoffs for CDMA Interference Cancellation (2006)

This paper investigates the design of commercially viable CDMA basestation receivers that incorporate interference cancellation. Results are given for cdma2000 1xEV-DO but also apply to WCDMA HSUPA. The effect of receiver memory size is determined for canceling packets transmitted with hybrid-ARQ. Performance-complexity tradeoffs are presented for implementing iterative and successive interference cancellation of asynchronous user transmissions. Multipath channel estimation techniques of varying complexity are compared based on sector throughput. Practical interference cancellation designs with moderate complexity are shown to achieve a large fraction of the gains of more elaborate techniques.
Modeling of the Starting Performance of Large Solid-pole Synchronous Motors Using Equivalent Circuit Approach (2007)

This paper presents the modeling of the starting performance of large solid pole synchronous motors using equivalent circuit approach. In this approach, the solid rotor body is assumed to have two sets of separate virtual windings. Therefore the two-axis theory can be used for the solid-pole synchronous motor. Parameters associated with the solid-pole are derived, together with their transformation coefficients. The starting performance was simulated and the results were validated by the test results on a 15000 Hp, 13.2 kV, 4 poles solid pole synchronous motor.
Intracellular vesicle entrapment of nanobubble ultrasound contrast agents targeted to PSMA promotes prolonged enhancement and stability in vivo and in vitro (2022)

Gas-core nanoscale bubbles (or nanobubbles) have gained significant recent attention as promising contrast agents for cancer molecular imaging using medical ultrasound. Previous work has shown that active targeting of nanobubbles to tumor biomarkers such as the prostate-specific membrane antigen (PSMA) significantly prolongs ultrasound signal enhancement, which is a critical feature for successful tumor diagnosis. However, the specific mechanism behind this effect is not well understood, and has not been previously studied in detail. Thus, in the current work, we investigated the process of PMSA- targeted nanobubble transport in tumors across different scales from <i>in vivo</i> whole tumor imaging using high-frequency dynamic contrast-enhanced ultrasound to intracellular confocal imaging and, molecularly using headspace gas chromatography/mass spectrometry. Data demonstrated that, indeed, molecular targeting of nanobubbles to the PSMA biomarker prolongs their tumor uptake and retention across the entire tumor volume, but with variability due to the expected tumor heterogeneity. Importantly, <i>in vitro</i>, the active targeting of NBs results in internalization via receptor-mediated endocytosis into the target cells, and the co-localization with intracellular vesicles (late-stage endosomes/lysosomes) significantly prolongs perfluorocarbon gas retention within the cells. This has not been directly observed previously. These results support the potential for nanobubbles to enable highly specific, background-free diagnostic imaging of the target cells/tissues using ultrasound.
Differential effects of brain‐derived neurotrophic factor and neurotrophin‐3 on hindlimb function in paraplegic rats (2011)

We compared the effect of viral administration of brain-derived neurotrophic factor (BDNF) or neurotrophin 3 (NT-3) on locomotor recovery in adult rats with complete thoracic (T10) spinal cord transection injuries, in order to determine the effect of chronic neurotrophin expression on spinal plasticity. At the time of injury, BDNF, NT-3 or green fluorescent protein (GFP) (control) was delivered to the lesion via adeno-associated virus (AAV) constructs. AAV-BDNF was significantly more effective than AAV-NT-3 in eliciting locomotion. In fact, AAV-BDNF-treated rats displayed plantar, weight-supported hindlimb stepping on a stationary platform, that is, without the assistance of a moving treadmill and without step training. Rats receiving AAV-NT-3 or AAV-GFP were incapable of hindlimb stepping during this task, despite provision of balance support. AAV-NT-3 treatment did promote the recovery of treadmill-assisted stepping, but this required continuous perineal stimulation. In addition, AAV-BDNF-treated rats were sensitized to noxious heat, whereas AAV-NT-3-treated and AAV-GFP-treated rats were not. Notably, AAV-BDNF-treated rats also developed hindlimb spasticity, detracting from its potential clinical applicability via the current viral delivery method. Intracellular recording from triceps surae motoneurons revealed that AAV-BDNF significantly reduced motoneuron rheobase, suggesting that AAV-BDNF promoted the recovery of over-ground stepping by enhancing neuronal excitability. Elevated nuclear c-Fos expression in interneurons located in the L2 intermediate zone after AAV-BDNF treatment indicated increased activation of interneurons in the vicinity of the locomotor central pattern generator. AAV-NT-3 treatment reduced motoneuron excitability, with little change in c-Fos expression. These results support the potential for BDNF delivery at the lesion site to reorganize locomotor circuits.
Growth Differentiation Factor 9 (GDF9) Stimulates Proliferation and Inhibits Steroidogenesis by Bovine Theca Cells: Influence of Follicle Size on Responses to GDF91 (2007)

Ovarian follicular development is controlled by numerous paracrine and endocrine regulators, including oocyte-derived growth differentiation factor 9 (GDF9), and a localized increase in bioavailable insulin-like growth factor 1 (IGF1). The effects of GDF9 on function of theca cells collected from small (3-6 mm) and large (8-22 mm) ovarian follicles were investigated. In small-follicle theca cells cultured in the presence of both LH and IGF1, GDF9 increased cell numbers and DNA synthesis, as measured by a (3)H-thymidine incorporation assay, and dose-dependently decreased both progesterone and androstenedione production. Theca cells from large follicles had little or no response to GDF9 in terms of cell proliferation or steroid production induced by IGF1. Small-follicle theca cell studies indicated that GDF9 decreased the abundance of LHR and CYP11A1 mRNA in theca cells, but had no effect on IGF1R, STAR, or CYP17A1 mRNA abundance or the percentage of cells staining for CYP17A1 proteins. GDF9 activated similar to mothers against decapentaplegics (SMAD) 2/3-induced CAGA promoter activity in transfected theca cells. Small-follicle theca cells had more ALK5 mRNA than large-follicle theca cells. Small-follicle granulosa cells appeared to have greater GDF9 mRNA abundance than large-follicle granulosa cells, but theca cells had no detectable GDF9 mRNA. We conclude that theca cells from small follicles are more responsive to GDF9 than those from large follicles and that GDF9 mRNA may be produced by granulosa cells in cattle. Because GDF9 increased theca cell proliferation and decreased theca cell steroidogenesis, oocyte- and granulosa cell-derived GDF9 may simultaneously promote theca cell proliferation and prevent premature differentiation of the theca interna during early follicle development.
A study of scaling and geometry effects on the forces between cuboidal and cylindrical magnets using analytical force solutions (2008)

Kelvin's formula is used to calculate forces acting on a permanent magnet in the presence of an external magnetic field from a second permanent magnet. This approach is used to derive explicit analytical solutions for the axial and lateral forces between cuboidal and cylindrical permanent magnets as functions of magnet dimensions and separation. While exact solutions can be found for cuboidal magnets, a hypergeometric expansion is used to approximate the elliptic integrals in solving for the fields and forces for the cylindrical magnets. The resulting equations are applied over a range of magnet sizes and geometries to explore scaling laws and other geometrical effects. It is shown that cuboidal magnets provide larger forces than equivalently sized cylindrical magnets. Also, the aspect ratio of the magnets significantly affects the forces. These results are intended to benefit the design and optimization of sensors, actuators and systems that rely on magnetic forces, particularly at the microscale.
A three-tier, tTCP/IP gateway architecture to link home networks with the internet. (1998)

non-peer-reviewed
Stereoelectronic Effects on Collagen Stability:  The Dichotomy of 4-Fluoroproline Diastereomers (2003)

Collagen is the most abundant protein in animals. Natural collagen consists of a triple helix of (Xaa-Yaa-Gly)n chains, in which the Xaa and Yaa residues are often l-proline. Here, a (2S,4S)-4-fluoroproline (flp) residue is shown to be greatly stabilizing in the Xaa position (but destabilizing in the Yaa position). In contrast, a (2S,4R)-4-fluoroproline (Flp) residue is shown to be greatly destabilizing in the Xaa position (but stabilizing in the Yaa position). The dichotomous effect of the diastereomers appears to arise from a gauche effect, which alters pyrrolidine ring pucker and hence properly (or improperly) preorganizes main-chain dihedral angles. Thus, the rational use of stereoelectronic effects can enhance the conformational stability of a protein.
Critical Path Heuristic for Automatic Parallelization (2008)

This paper presents an algorithm to heuristically partition the data dependence graph representing a basic block or a hyper block of code in order to assign instructions to available processing cores. The algorithm relies on the observation that the critical path in the data dependence graph defines the lower bound on the schedule length of a block and hence the instructions on the critical path should be assigned to the same core. The algorithm has been implemented in the Trimaran compiler framework. The given heuristic algorithm is much simpler and evaluation shows that it is as good as previous state-of-the-art heuristic algorithms.
Erratum: Nanofabrication using structure controlled hydrogenated Si clusters deposited on Si surfaces [J. Vac. Sci. Technol. B 18, 3497 (2000)] (2004)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation Toshihiko Kanayama, Miyoko O. Watanabe, Lonid Bolotov, Noriyuki Uchida; Erratum: Nanofabrication using structure controlled hydrogenated Si clusters deposited on Si surfaces [J. Vac. Sci. Technol. B 18, 3497 (2000)]. Journal of Vacuum Science & Technology B: Microelectronics and Nanometer Structures Processing, Measurement, and Phenomena 1 November 2004; 22 (6): 2863. https://doi.org/10.1116/1.1825021 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAVS: Science & Technology of Materials Interfaces and ProcessingJournal of Vacuum Science & Technology B Search Advanced Search |Citation Search
Fabrication of a Low-Frequency Ultrasonic Transducer (2008)

S.443-446