gebhart/patrepeval huggingface dataset:
IPv6-based discovery and association of Virtualization Infrastructure Manager (VIM) and Network Function Virtualization Orchestrator (NFVO) (2020)

Virtualized resources do not need to be limited to those available in
traditional data centers, where the infrastructure is stable, static,
typically homogeneous and managed by a single admin entity.
Computational capabilities are becoming more and more ubiquitous, with
terminal devices getting extremely powerful, as well as other types of
devices that are close to the end users at the edge (e.g., vehicular
onboard devices for infotainment, micro data centers deployed at the
edge, etc.). It is envisioned that these devices would be able to
offer storage, computing and networking resources to nearby network
infrastructure, devices and things (the fog paradigm). These resources
can be used to host functions, for example to offload/complement other
resources available at traditional data centers, but also to reduce
the end-to-end latency or to provide access to specialized information
(e.g., context available at the edge) or hardware. This document
describes mechanisms allowing dynamic discovery of virtualization
resources and orchestrators in IPv6-based networks. In the current
version, mechanisms based on piggybacking options on IPv6 neighbor
discovery are explored. New IPv6 neighbor discovery options are
defined. Additional mechanisms will be explored in future releases of
this document.
Targeting metabolic driving and intermediate influx in lysine catabolism for high-level glutarate production (2019)

Various biosynthetic pathways have been designed to explore sustainable production of glutarate, an attractive C5 building block of polyesters and polyamides. However, its efficient production has not been achieved in Escherichia coli. Here, we use E. coli native lysine catabolic machinery for glutarate biosynthesis. This endogenous genes-only design can generate strong metabolic driving force to maximize carbon flux toward glutarate biosynthesis by replenishing glutamate and NAD(P)H for lysine biosynthesis, releasing lysine feedback inhibition, and boosting oxaloacetate supply. We use native transporters to overcome extracellular accumulation of cadaverine and 5-aminovalerate. With these efforts, both high titer (54.5 g L<sup>-1</sup>) and high yield (0.54 mol mol<sup>-1</sup> glucose) of glutarate production are achieved under fed-batch conditions. This work demonstrates the power of redirecting carbon flux and the role of transporters to decrease intermediate accumulation.
A viral kinase mimics S6 kinase to enhance cell proliferation (2020)

Viruses usurp the host cell machinery to replicate, disseminate, and propagate themselves. Kaposi’s sarcoma-associated herpesvirus (KSHV) encodes a viral protein kinase (vPK) also known as ORF36. Using in silico modeling and biochemistry, we report that vPK/ORF36 displays limited homology to cellular S6 kinase B1 (S6KB1). Both kinases share overlapping substrates and can phosphorylate S6. However, unlike S6KB1, vPK augments S6 phosphorylation under conditions where mammalian target of rapamycin (mTOR) is inhibited. vPK modulates cellular proliferation and protein synthesis, augments anchorage independence, and enhances angiogenesis. Depletion of vPK/ORF36 during lytic replication inhibits the production of infectious virions, thereby underscoring the importance of this kinase during the KSHV life cycle. Our collective observations suggest that vPK may function as a constitutively active mimic of S6KB1.
Feasibility Study of Ion-Cut InP Photoconductor Devices on Glass Substrates (2009)

Photoconductive devices, prefabricated on semi-insulating InP, were integrated on glass substrates using hydrogen-induced layer transfer (ion-cut) combined with adhesive bonding. The prefabricated photodetectors were implanted through by hydrogen ions and transferred twice to achieve device-up configuration. The transferred devices exhibited both higher photocurrent and dark current when compared with bulk devices. Transient response measurement showed a long-lived tail that persisted after the initial "fast" response, indicative of trap-mediated transport due to the implant-related localized states. Our study showed that the ion-cutting layer transfer process yielded functional photodetectors. The residual defects remain a challenging issue for the ion-cut process.
Bilateral Ureteral Obstruction After Repair of Aortic Aneurysm (1979)

No AccessJournal of Urology1 Aug 1979Bilateral Ureteral Obstruction After Repair of Aortic Aneurysm Jacob Rajfer, and Gardner W. Smith Jacob RajferJacob Rajfer More articles by this author , and Gardner W. SmithGardner W. Smith More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)56353-3AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Ureteral obstruction is a rare complication after a reconstructive vascular operation. We report a case of bilateral ureteral obstruction leading to anuria 8 months after the repair of an infrarenal abdominal aortic aneurysm and placement of an aortoiliac bypass graft. © 1979 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited ByBlasco F and Saladié J (2018) Ureteral Obstruction and Ureteral Fistulas After Aortofemoral or Aortoiliac Bypass SurgeryJournal of Urology, VOL. 145, NO. 2, (237-242), Online publication date: 1-Feb-1991.Goldenberg S, Gordon P, Cooperberg P and McLoughlin M (2018) Early Hydronephrosis following Aortic Bifurcation Graft Surgery: A Prospective StudyJournal of Urology, VOL. 140, NO. 6, (1367-1369), Online publication date: 1-Dec-1988.Noviek A (2018) Editorial CommentJournal of Urology, VOL. 129, NO. 1, (16-21), Online publication date: 1-Jan-1983.O’mara C (2018) Editorial CommentJournal of Urology, VOL. 129, NO. 1, (21-22), Online publication date: 1-Jan-1983. Volume 122Issue 2August 1979Page: 255-256 Advertisement Copyright & Permissions© 1979 by The American Urological Association Education and Research, Inc.MetricsAuthor Information Jacob Rajfer More articles by this author Gardner W. Smith More articles by this author Expand All Advertisement PDF DownloadLoading ...
Effects and feasibility of virtual reality system vs traditional physical therapy training in multiple sclerosis patients (2018)

Background/Aims: Traditionally, physical therapy for gait and balance training takes place in a gym setting, which may not fully reproduce situations in everyday activities. The Computer Assisted Rehabilitation ENvironment system provides an immersive virtual environment, allowing a simulation of complex conditions conducive to gait and balance training. The purpose of this study was to determine: (1) the feasibility and safety of using the Computer Assisted Rehabilitation ENvironment system for physical therapy sessions with multiple sclerosis patients, and (2) pre-post treatment changes observed with the Computer Assisted Rehabilitation ENvironment system compared to traditional physical therapy. Findings: Data from functional outcome measures Berg Balance Scale, Timed Up and Go test, Timed 25-Foot Walk, and 6-Minute Walk Test were extracted from the medical records of 62 patients with multiple sclerosis who had at least three physical therapy sessions in either environment. Statistically significant within-group improvements were observed for all outcome measures in the physical therapy Computer Assisted Rehabilitation ENvironment system group (P&lt;0.05), and only for Berg Balance Scale in the traditional physical therapy group. There was a significant between-group difference in favour of physical therapy with the Computer Assisted Rehabilitation ENvironment system for the Timed 25-Foot Walk (P=0.022). Conclusions: The results suggest the Computer Assisted Rehabilitation ENvironment system is a safe and effective tool for physical therapy-led gait and balance training for individuals with multiple sclerosis.
Corrosion Resistance of Pipeline Steel with Damaged Enamel Coating and Cathodic Protection (2018)

This paper presents the first report on the corrosion resistance of pipeline steel with damaged enamel coating and cathodic protection in 3.5 wt % NaCl solution. In particular, dual cells are set up to separate the solution in contact with the damaged and intact enamel coating areas, to produce a local corrosion resistance measurement for the first time. Enamel-coated steel samples, with two levels of cathodic protection, are tested to investigate their impedance by electrochemical impedance spectroscopy (EIS) and their cathodic current demand by a potentiostatic test. Due to its glass transition temperature, the enamel-coated pipeline can be operated on at temperatures up to 400 °C. The electrochemical tests show that cathodic protection (CP) can decelerate the degradation process of intact coating and delay the electrochemical reactions at the enamel-steel interface. However, CP has little effect on the performance of coating once damaged and can prevent the exposed steel from corrosion around the damaged site, as verified by visual inspections. Scanning electron microscopy (SEM) indicated no delamination at the damaged enamel–steel interface due to their chemical bond.
Analysis of Optimal Diagonal Loading for MPDR-Based Spatial Power Estimators in the Snapshot Deficient Regime (2018)

The minimum power distortionless response (MPDR) beamformer minimizes the output power while passing the look direction signal with unity gain. To alleviate the performance degradation caused by estimating the spatial correlation matrix with a relatively small number of snapshots of the received signal compared to the number of sensors, a regularization implemented via diagonal loading of the estimated correlation matrix is used. This paper presents a study for the optimal diagonal loading that minimizes the estimation mean square error (MSE) of two diagonally loaded MPDR beamformer-based spatial power estimators in the snapshot deficient regime. First, the asymptotic behavior of the power estimators for fixed diagonal loading is analyzed and the approximate characterization of their expectations is derived. Second, it is conjectured that because of the snapshot deficient sample support, the squared bias is the factor that primarily controls the optimal diagonal loading. Finally, the respective performances of the two power estimators are compared using MSE as the metric and it is shown that one outperforms the other. The analytical results are validated using simulation data.
Benchmarking a fluorescence microplate‐based aggregation assay using subvisible protein particle standards (2011)

Aggregation represents one of the most significant obstacles to protein‐based pharmaceutical development. During drug formulation, aggregation can impact product quality in terms of biological activity and immunogenicity. Improved methods are required to detect and monitor aggregation during manufacturing. We report upon a fluorescence microplate assay employing a novel fluorescent probe specifically devised to detect aggregated proteins and peptides. The probe is nonfluorescent in solution, but becomes brightly fluorescent when bound to the tertiary structure of aggregated proteins. The assay is capable of detecting less than 0.2% aggregated protein in a concentrated antibody formulation, with a linear dynamic range of two‐orders of magnitude. Sub‐visible oligomeric particles are readily detected without a need for sample separation, solvent exchange or dilution. Subvisible protein particle standards were developed to benchmark the fluorescence microplate assay, as well as orthogonal characterization methods, including laser‐based nanoparticle tracking and micro‐flow Imaging. Advantages of the microplate‐based assay include simple implementation with widely available instrumentation, results achievable in 30 minutes or less and robust assay performance. The assay provides a convenient, complementary orthogonal method for cross‐validation of instrument‐intensive techniques. This work was supported by ENZO Life Sciences.
Accelerating the early angiogenesis of tissue engineering constructs in vivo by the use of stem cells cultured in matrigel (2013)

In tissue engineering research, generating constructs with an adequate extent of clinical applications remains a major challenge. In this context, rapid blood vessel ingrowth in the transplanted tissue engineering constructs is the key factor for successful incorporation. To accelerate the microvascular development in engineered tissues, we preincubated osteoblast-like cells as well as mesenchymal stem cells or a combination of both cell types in Matrigel-filled PLGA scaffolds before transplantation into the dorsal skinfold chambers of balb/c mice. By the use of preincubated mesenchymal stem cells, a significantly accelerated angiogenesis was achieved. Compared with previous studies that showed a decisive increase of vascularization on day 6 after the implantation, we were able to halve this period and achieve explicitly denser microvascular networks 3 days after transplantation of the tissue engineering constructs. Thereby, the inflammatory host tissue response was acceptable and low, comparable with former investigations. A co-incubation of osteoblast-like cells and stem cells showed no additive effect on the density of the newly formed microvascular network. Preincubation of mesenchymal stem cells in Matrigel is a promising approach to develop rapid microvascular growth into tissue engineering constructs. After the implantation into the host organism, scaffolds comprising stem cells generate microvascular capillary-like structures exceptionally fast. Thereby, transplanted stem cells likely differentiate into vessel-associated cells. For this reason, preincubation of mesenchymal stem cells in nutrient solutions supporting different steps of angiogenesis provides a technique to promote the routine use of tissue engineering in the clinic.
Acellular Adipose Tissue Scaffold for Soft Tissue Reconstruction (2011)

Plastic and Reconstructive Surgery: October 2011 - Volume 128 - Issue Supplement 4S - p 83-84 doi: 10.1097/01.prs.0000406313.33059.f8
Front Matter: Volume 9781 (2016)

This PDF file contains the front matter associated with SPIE Proceedings Volume 9781, including the Title Page, Copyright information, Table of Contents, Introduction (if any), and Conference Committee listing.
Binuclear Two-Coordinate Coinage Metal Complexes (2023)

The model for rational design of carbene metal amide (cMa) complexes with large oscillator strengths while also decreasing the energy of the singlet and triplet splitting (dEST) is presented. A series of binuclear cMa complexes bearing Janus carbenes have been synthesized and studied. The binuclear cMa complexes exhibit short lifetimes (tau = 200-300 ns) with high photoluminescence efficiencies (PL >95%). The radiative rates of binuclear cMa complexes are 3~4 times faster than that of the corresponding mononuclear cMa complexes. Analysis of temperature-dependent luminescence data indicates that the fluorescent lifetime for the singlet state (tS1) of binuclear cMa complexes are near 12 ns with a dEST of 40-50 meV. The presented compounds provide a general design for cMa complexes to achieve small values for dEST while retaining high radiative rates.
Glucose and Nutrient Concentrations Affect the Expression of a 104-Kilodalton Listeria Adhesion Protein in Listeria monocytogenes (2002)

Growth media and environmental conditions influence the expression of adhesion and invasion proteins in Listeria monocytogenes. Here, the expression of the 104-kDa Listeria adhesion protein (LAP) was studied in nutrient-rich media (Trypticase soy broth [TSB] and brain heart infusion [BHI]), minimal medium (Luria-Bertani [LB]), or nutrient-deficient medium (peptone water [PW]) by immunoblotting, enzyme-linked immunosorbent assay (ELISA), and immunoelectron microscopy. Also, the effect of incorporating different concentrations of glucose on LAP expression was studied. Immunoblotting showed that LAP expression was at least twofold higher in LB medium than in TSB or BHI, while PW supported very poor cell growth and LAP expression. ELISA and immunoblotting results showed that higher concentrations of glucose (>1.6 g/liter) lowered the culture pH and suppressed LAP expression by more than 75%; however, the addition of K(2)HPO(4) reduced this effect. L. monocytogenes cells grown in LB media with lower concentrations of glucose showed higher adhesion to Caco-2 cells (3,716 and 4,186 cpm of attached bacteria for 0 and 0.2 g of glucose/liter, respectively), while L. monocytogenes cells grown in LB with higher glucose concentrations exhibited lower adhesion (2,126 and 2,221 cpm for 1.6 and 3.2 g of glucose/liter, respectively). A LAP-negative L. monocytogenes strain (A572) showed low adhesion profiles regardless of the amount of glucose added. Transmission electron microscopy revealed that LAP is localized mainly in the cytoplasm, with only a few molecules located on the cell surface. Growth in LB with high glucose (3.2 g/liter) showed the presence of only a few molecules in the cells, corroborating the results observed with ELISA or immunoblotting. In summary, nutrient-rich media and high concentrations of glucose suppressed LAP expression, which possibly is due to the changes in the pH of the media during growth from the accumulation of sugar fermentation by-products.
Abstract P459: The BBSome Mediate the Sorting of the Serotonin 5-HT2C Receptor to the Plasma Membrane in POMC Neurons (2017)

The BBSome, a multiplex of 8 Bardet-Biedl Syndrome (BBS) proteins including BBS1, has emerged as an important regulator of energy homeostasis and cardiovascular function. Disrupting the BBSome, through deletion of its units, globally, in the nervous system, hypothalamus or selective neurons such as the proopiomelanocortin (POMC) neurons causes obesity associated with sympathetic overdrive and hypertension. However, the mechanisms underlying these phenotypes evoked by BBSome disruption remain ill defined. Serotonin 5-HT2C receptor (5-HT2CR) present in POMC neurons plays an important role in the regulation of energy homeostasis. We hypothesized that the BBSome contribute to the regulation of 5-HT2CR signaling. Given the importance of the BSBome for the trafficking of proteins to cilia, we first assessed whether 5-HT2CR localize the cilium in mouse MICD cells transfected with GFP-tagged 5-HT2CR. We found 5-HT2CR present in the plasma membrane, but no evidence for ciliary localization of this receptor. To study the relevance of the BBSome for the trafficking of 5-HT2CR, we deleted the Bbs1 gene in IMCD cells using CRISP-Cas9. We found that the 5-HT2CR failed to traffic to the plasma membrane in IMCD cells lacking the Bbs1 gene. Moreover, the increase in intracellular calcium and membrane potential evoked by serotonin were significantly reduced in cells lacking the Bbs1 gene. Interestingly, double immunostaining revealed that most of 5-HT2CR was stuck in the late endosome co-localizing with CD63, a marker of this organelle. Next, we analyzed the localization of the 5-HT2CR in the hypothalamic arcuate nucleus of mice lacking the Bbs1 gene selectively in POMC neurons (Bbs1 POMC ). In control mice, 5-HT2CR was predominantly present in the plasma membrane of POMC neurons and in some non-POMC cells. In Bbs1 POMC mice, 5-HT2CR was altered selectively in POMC neurons appearing stalled in the cytoplasm. Finally, i.p. administration of lorcaserin, a 5-HT2C specific agonist, caused a significant decrease in body weight and food intake in control mice, but not in Bbs1 POMC mice. These findings demonstrate that the BBSome underlie the trafficking of 5-HT2CR in POMC neurons with important implications for the regulation of energy homeostasis and development of obesity.
DeltaCNN: End-to-End CNN Inference of Sparse Frame Differences in Videos (2022)

Convolutional neural network inference on video data requires powerful hardware for real-time processing. Given the inherent coherence across consecutive frames, large parts of a video typically change little. By skipping identical image regions and truncating insignificant pixel updates, computational redundancy can in theory be reduced significantly. However, these theoretical savings have been difficult to translate into practice, as sparse updates hamper computational consistency and memory access coherence; which are key for efficiency on real hardware. With DeltaCNN, we present a sparse convolutional neural network framework that enables sparse frame-by-frame updates to accelerate video inference in practice. We provide sparse implementations for all typical CNN layers and propagate sparse feature updates end-to-end – without accumulating errors over time. DeltaCNN is applicable to all convolutional neural networks without retraining. To the best of our knowledge, we are the first to significantly outperform the dense reference, cuDNN, in practical settings, achieving speedups of up to 7x with only marginal differences in accuracy. Our CUDA kernels and PyTorch extensions can be found at https://github.com/facebookresearch/DeltaCNN.
Characterization of the cysJIH Regions of Salmonella typhimurium and Escherichia coli B (1989)

The hemoprotein component of Salmonella typhimurium sulfite reductase (NADPH) (EC 1.8.1.2) was purified to homogeneity from cysJ266, a mutant strain lacking sulfite reductase flavoprotein. The siroheme- and Fe4S4-containing enzyme was isolated as a monomeric 63-kDa polypeptide and consisted of a mixture of unligated enzyme and a complex with sulfite. Following reduction with 5'-deazaflavin-EDTA and reoxidation, the complex was converted to the uncomplexed, high spin ferri-siroheme state seen previously with Escherichia coli sulfite reductase hemoprotein preparations. The S. typhimurium hemoprotein exhibited catalytic and physical properties identical to the hemoprotein prepared by urea dissociation of E. coli sulfite reductase holoenzyme and was fully competent in reconstituting NADPH-sulfite reductase activity when combined with excess purified sulfite reductase flavoprotein. The DNA sequences of cysI and cysH from S. typhimurium and E. coli B were determined and, together with previously reported data, confirmed the organization of this region as promoter-cysJ-cysI-cysH with all three genes oriented in the same direction from the promoter. Molecular weights deduced for the cysI-encoded sulfite reductase hemoprotein and for the cysH-encoded 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase were approximately 64,000 and 28,000, respectively. Comparison of the deduced amino acid sequence of sulfite reductase hemoprotein with that of spinach nitrite reductase (Back, E., Burkhart, W., Moyer, M., Privalle, L., and Rothstein, S. (1988) Mol. Gen. Genet. 212, 20-26), which also contains siroheme and an Fe4S4 cluster, showed two groups of cysteine-containing sequences with the structures Cys-(X)3-Cys and Cys-(X)5-Cys, which are homologous in the two enzymes and are postulated to provide the ligands of the Fe4S4 cluster in both proteins. From these sequences and from crystallographic (McRee, D. E., Richardson, D. C., Richardson, J. S., and Siegel, L. M. (1986) J. Biol. Chem. 261, 10277-10281) and spectroscopic data in the literature, a model is proposed for the structure of the active center of these two enzymes.