gebhart/patrepeval huggingface dataset:
Reactions of vinyl ethers with cobalamins and cobaloximes (1974)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTReactions of vinyl ethers with cobalamins and cobaloximesRichard B. Silverman and David DolphinCite this: J. Am. Chem. Soc. 1974, 96, 22, 7094–7096Publication Date (Print):October 1, 1974Publication History Published online1 May 2002Published inissue 1 October 1974https://pubs.acs.org/doi/10.1021/ja00829a045https://doi.org/10.1021/ja00829a045research-articleACS PublicationsRequest reuse permissionsArticle Views119Altmetric-Citations20LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Low Compexity Motion Analysis for Mobile Video Devices (2007)

This paper describes a low-complexity method for estimation of translational motion in video sequences, based on the principle that 2D image translation results in translation of image projections. The method uses a single pixel-by-pixel frame scan to extract a compact frame-descriptor. The translation between frames is determined by matching frame-descriptors using fixed-point implementation of the phase correlation method (PCM). The method gives reliable results even when video images are blurred by significant and rapid motion and in the presence of independent local motions. Real-time performance has been achieved in a low-cost DSP board.
Investigation of the inframolecular acid–base properties of d-myo-inositol 1,3,4,5-tetrakisphosphate and dl-myo-inositol 1,2,4,5-tetrakisphosphate (1997)

The protonation processes of the individual phosphate groups in two regioisomeric inositol polyphosphates, D-myo-inositol 1,3,4,5-tetrakisphosphate and DL-myo-inositol 1,2,4,5-tetrakisphosphate, have been investigated by 31 P NMR titration experiments; the results are compared to those from D-myo-inositol 1,4,5-trisphosphate.
High Capacity Electric A/C Compressor with Integrated Inverter for Hybrid Automotive and Commercial Vehicles (2008)

&lt;div class="htmlview paragraph"&gt;The market growth for electric-hybrid passenger vehicles has been very significant and is expected to reach nearly 25% of all vehicles sold in the US by 2015. Hybrid commercial vehicles are also being developed by several OEM's. This paper discusses the progress of Delphi Thermal Systems in developing an integrated electric compressor drive with high cooling capacity (9 kW+), sufficient for large hybrid SUV's and commercial vehicles such as Class 8 tractors with sleeper. An important driver for use of the electric compressor in the hybrid truck application is the reduction of engine idling time while maintaining comfort in the cab or sleeper. Design details of a compact 5 kW SPM motor, its inverter drive, and issues related to its integration into the compressor housing are described. Test results are given confirming excellent performance.&lt;/div&gt;
Subspace correction multi‐level methods for elliptic eigenvalue problems (2001)

Abstract In this work, we apply the ideas of domain decomposition and multi‐grid methods to PDE‐based eigenvalue problems represented in two equivalent variational formulations. To find the lowest eigenpair, we use a “subspace correction” framework for deriving the multiplicative algorithm for minimizing the Rayleigh quotient of the current iteration. By considering an equivalent minimization formulation proposed by Mathew and Reddy, we can use the theory of multiplicative Schwarz algorithms for non‐linear optimization developed by Tai and Espedal to analyse the convergence properties of the proposed algorithm. We discuss the application of the multiplicative algorithm to the problem of simultaneous computation of several eigenfunctions also formulated in a variational form. Numerical results are presented. Copyright © 2001 John Wiley &amp; Sons, Ltd.
Nanolithography (1996)

Nanolithography is the technology of fabricating sub- structures for use in either electronic devices or novel experiments in physics. While this has predominantly been the province of electron-beam lithography, new approaches explore the use of scanning probe techniques. Here, we discuss the limitations of electron-beam lithography, the development of novel resists, and some uses of scanning-probe lithography.
Molecular mechanisms involved in the regulation of the cytochrome P450 epoxygenase CYP2J5 (2008)

CYP2J5 is expressed in kidney and liver, and metabolizes arachidonic acid to epoxyeicosatrienoic acids. Herein, we identify the molecular mechanisms responsible for regulation of this gene. The transcriptional start site of the Cyp2j5 gene was located and analysis of the proximal promoter region identified a putative HNF1alpha binding site and a putative androgen response element (ARE). Electrophoretic mobility shift assays (EMSAs) demonstrated binding of androgen receptor (AR) to the Cyp2j5 ARE. Chromatin immunoprecipitation assays with mouse kidney cells confirmed specific binding of AR to the Cyp2j5 ARE, and this was increased in cells treated with androgen compared to cells treated with vehicle. EMSAs also showed the binding of HNF1alpha to the putative Cyp2j5 binding site. Reporter assays with a Cyp2j5 promoter vector demonstrated activation when an HNF1alpha overexpression vector was co‐transfected but not when there was a mutation in the HNF1alpha site. Immunohistochemistry demonstrated a marked reduction in the expression of Cyp2j5 in livers but only minimal changes in kidneys of HNF1alpha knockout mice relative to wild type controls. These findings provide a molecular basis for the observed gender and tissue specific differences in the expression of the Cyp2j5 gene. This research was supported by the Intramural Research Program of the NIH, National Institute of Environmental Health Sciences.
Suitability of high-numerical-aperture i-line steppers with oblique illumination for linewidth control in 0.35-μm complex circuit patterns (1993)

A 2-dimensional scalar aerial image model was used to computationally study i-line imaging with oblique illumination. Limited comparisons between developed photoresist images and aerial images were made. The effects on CD control, exposure latitude, and bias of varying annular and quadrupole geometry were mapped via simulation. Significant improvements in DOF of isolated lines was achieved with oblique illumination. Isolated line to dense line bias could be adjusted by changing the illumination type. Although oblique illumination improved the aerial image contrast at defocus, it caused degradation in the aerial image contrast at best focus. Long and short range proximity effects degraded the simulated CD control of optimized oblique illumination systems. This was observed in simulations of an SRAM gate cell. The imaging performance at .9 micrometers defocus, of an i-line system (NA .48) with oblique illumination, was judged to be worse than a KrF system (NA .42) with standard illumination. Quadrupole illumination was not found to measurably affect lens distortion.
Functional Studies of Aromatase Activity in Human Granulosa Cells from Normal and Polycystic Ovaries* (1979)

To determine the basis of the reduced ovarian estrogen (E) production in polycystic ovary syndrome (PCO), the ability of granulosa cells from normal and polycystic ovaries to aromatize androgens was assessed both in vitro and in vivo. During a 5-h incubation in vitro with graded doses of aromatase substrate [androstenedione (Δ4), 10−10−10−6 M], a dose-related increase in E production was observed in granulosa cells from normal 8- to 15-mm follicles; the minimum and maximum effective doses of Δ4 were 10−10 and 10−7 M, respectively, and the ED50 was approximately 10−8 M. In contrast, granulosa cells from 4- to 6-mm follicles of both normal and polycystic ovaries produced negligible amounts of E when incubated 5 h with graded doses of Δ4,suggesting that the aromatase enzyme had not been induced in follicles of this size. To test the functional relationship between gonadotropins and E production in PCO and normal granulosa cells from the aromatase-deficient 4- to 6-mm follicles, these granulosa cells were cultured in a chemically defined medium for 48 h with Δ4 (10−7 M) and FSH (100 ng/ml) or human LH (100 ng/ml). Cultured granulosa cells from both PCO and normal subjects responded significantly to FSH by showing 24- and 17-fold increases in E production, respectively. LH had little or no effect. Similarly, administration of human FSH to patients in vivo resulted in a rapid and dramatic increase in plasma estradiol and estrone levels. These results indicate that granulosa cells from PCO patients have an inherent ability to respond to the FSH induction of aromatase activity, but the Graafian follicles never develop to the size in which the aromatase enzyme is normally expressed. Such data strongly suggest that the absence of aromatase in PCO and the arrested follicle growth may be caused by a reduced local concentration of FSH and/or decreased bioactivity.
ChemInform Abstract: Catalytic Direct Acetoxylation Of Indoles. (2010)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Distinct regulation of pHin and [Ca2+]in in human melanoma cells with different metastatic potential (1998)

We investigated whether alterations in the mechanisms involved in intracellular pH (pHin) and intracellular calcium ([Ca2+]in) homeostasis are associated with the metastatic potential of poorly (A375P) and highly (C8161) metastatic human melanoma cells. We monitored pHin and [Ca2+]in simultaneously, using the fluorescence of SNARF-1 and Fura-2, respectively. Our results indicated that steady-state pHin and [Ca2+]in between these cell types were not significantly different. Treatment of cells with NH4Cl resulted in larger pHin increases in highly than in poorly metastatic cells, suggesting that C8161 cells have a lower H+ buffering capacity than A375P. NH4Cl treatment also increased [Ca2+]in only in C8161 cells. To determine if the changes in [Ca2+]in triggered by NH4Cl treatment were due to alterations in either H+- or Ca2+-buffering capacity, cells were treated with the Ca2+-ionophore 4Br-A23187, to alter [Ca2+]in. The magnitude of the ionophore-induced [Ca2+]in increase was slightly greater in C8161 cells than in A375P. Moreover, A375P cells recover from the ionophore-induced [Ca2+]in load, whereas C8161 cells did not, suggesting that A375P may exhibit distinct [Ca2+]in regulatory mechanisms than C8161 cells, to recover from Ca2+ loads. Removal of extracellular Ca2+ ([Ca2+]ex) decreased [Ca2+]in in both cell types at the same extent. Ionophore treatment in the absence of [Ca2+]ex transiently increased [Ca2+]in in C8161, but not in A375P cells. Endoplasmic reticulum (ER) Ca2+-ATPase inhibitors such as cyclopiazonic acid (CPA) and thapsigargin (TG) increased steady-state [Ca2+]in only in C8161 cells. Together, these data suggest that the contribution of intracellular Ca2+ stores for [Ca2+]in homeostasis is greater in highly than in poorly metastatic cells. Bafilomycin treatment, to inhibit V-type H+-ATPases, corroborated our previous results that V-H+-ATPases are functionally expressed at the plasma membranes of highly metastatic, but not in poorly metastatic cells (Martínez-Zaguilán et al., 1993). Collectively, these data suggest that distinct pHin and [Ca2+]in regulatory mechanisms are present in poorly and highly metastatic human melanoma cells.
Design and characterization of high-voltage self-clamped IGBT's (1999)

A new concept of field limiting ring (FLR) with variable ring widths is proposed for designing a high-voltage collector-gate clamped IGBT. An insulated gate bipolar transistor (IGBT) based on the concept has been designed and fabricated with a conventional IGBT process flow to provide a clamp voltage of 620 V. The dc and unclamped inductive switching (UIS) energy parameters of the IGBT are fully characterized for a temperature range of -40-150/spl deg/C. The new high-voltage self-clamped IGBT is to be primarily used in automotive ignition applications.
Comprehensive list of SUMO targets in Caenorhabditis elegans and its implication for evolutionary conservation of SUMO signaling (2018)

Post-translational modification by small ubiquitin-related modifier (SUMO) is a key regulator of cell physiology, modulating protein-protein and protein-DNA interactions. Recently, SUMO modifications were postulated to be involved in response to various stress stimuli. We aimed to identify the near complete set of proteins modified by SUMO and the dynamics of the modification in stress conditions in the higher eukaryote, Caenorhabditis elegans. We identified 874 proteins modified by SUMO in the worm. We have analyzed the SUMO modification in stress conditions including heat shock, DNA damage, arsenite induced cellular stress, ER and osmotic stress. In all these conditions the global levels of SUMOylation was significantly increased. These results show the evolutionary conservation of SUMO modifications in reaction to stress. Our analysis showed that SUMO targets are highly conserved throughout species. By comparing the SUMO targets among species, we approximated the total number of proteins modified in a given proteome to be at least 15-20%. We developed a web server designed for convenient prediction of potential SUMO modification based on experimental evidences in other species.
Delta relaxation enhanced MR: Improving activation‐specificity of molecular probes through R1 dispersion imaging (2009)

MR molecular imaging enables high-resolution, in vivo study of molecular processes frequently utilizing gadolinium-based probes that specifically bind to a particular biological molecule or tissue. While some MR probes are inactive when unbound and produce enhancement only after binding, the majority are less specific and cause enhancement in either state. Accumulation processes are then required to increase probe concentration in regions of the target molecule/tissue. Herein, a method is described for creating specificity for traditionally nonspecific probes. This method utilizes MR field-cycling methods to produce MRI contrast related to the dependence of R(1) upon magnetic field. It is shown that the partial derivative of R(1) with respect to magnetic field strength, R(1)', can be used as an unambiguous measure of probe binding. T(1)-weighted images and R(1)' images were produced for samples of albumin and buffer both enhanced with the albumin-binding agent Vasovist. For T(1) images, samples with low concentrations of Vasovist in an albumin solution could not be differentiated from samples with higher concentrations of Vasovist in buffer. Conversely, the R(1)' images showed high specificity to albumin. Albumin samples with a 10-microM concentration of Vasovist were enhanced over buffer samples containing up to 16 times more Vasovist.
New insights into the cell biology of ischemic acute renal failure. (1991)

Proximal tubule cells play an essential role in the reabsorption of ions, water, and solutes from the glomerular filtrate. This is accomplished, in large part, by having a surface membrane polarized into structurally, biochemically, and physiologically distinct apical and basolateral membrane domains separated by cellular junctional complexes. Establishment and maintenance of these unique membrane domains are essential for the normal functioning of the cell. Ischemia results in the duration-dependent loss of apical and basolateral surface membrane lipid and protein polarity. Loss of surface membrane polarity is preceded by disruption of the microfilament network and opening of cellular tight junctions. Surface membrane lipids and proteins are then free to diffuse laterally within the bilayer into the alternate membrane domain. Functionally, ischemia-induced loss of epithelial polarity has been shown to be responsible for reduced sodium and glucose reabsorption. Reduced Na+ reabsorption has been related to redistribution of Na+, K(+)-ATPase into the apical membrane. During recovery from ischemic injury, proximal tubule cells undergo remodeling of the surface membrane such that the unique apical and basolateral membrane domains are reestablished, allowing for the return of normal cellular function.
An Efficient Synthesis of Difluoropropargyl Bromides. (2006)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Hydrogenogenic and sulfidogenic growth of Thermococcus archaea on carbon monoxide and formate (2016)

Enrichment and pure cultures of hyperthermophilic archaea capable of anaerobic growth on one- carbon compounds (CO and/or formate) were obtained from deep-sea sites of hydrothermal activity at the Mid-Atlantic Ridge, Lau Basin, and Guaymas Basin. All isolates belonged to the T barophilus-T paralvi- .nellae group within the genus Thermococcus. In all cases available for analysis, the genomes of Thermococcus strains capable of growth by hydrogenogenic utilization of CO and/or formate contained clusters of genes en- coding energy-converting hydrogenase and either CO dehydrogenase or formate dehydrogenase and formate transporter. Apart from the previously known processes of hydrogenogenic oxidation of CO and formate, the oxidation of these substrates coupled to sulfur reduction was observed, processes previously unknown among archaea. The capacities for hydrogenogenic or sulfidogenic oxidation of CO and formate occurred in the studied strains in all possible combinations, which could only in part be explained by peculiarities of organi- zation of genetic determinants revealed in the genomes. Investigation of CO and formate consumption kinet- ics revealed that T barophilus strain Ch5 was able to grow at concentrations close to the environmental ones. Thus, it was shown that hyperthermophilic archaea from deep-sea hydrothermal vents are able to utilize one- carbon substrates of abiotic origin both in the presence of an electron acceptor (sulfur) and in its absence. These processes were probably of importance under the conditions of the early Earth biosphere.
Down-regulation of adhesion molecules by oncogenes (1998)

Epithelìal Cell Biology Laboratory, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London WC2A 3PX, UK
Expansion of Lithium Ion Pouch Cell Batteries: Observations from Neutron Imaging (2013)

The expansion of battery material during lithium intercalation is a concern for the cycle life and performance of lithium ion batteries. In this paper, electrode expansion is quantified from in situ neutron images taken during cycling of pouch cells with lithium iron phosphate positive and graphite negative electrodes. Apart from confirming the overall expansion as a function of state of charge and the correlation with graphite transitions that have been observed in previous dilatometer experiments we show the spatial distribution of the expansion along the individual electrodes of the pouch cell. The experiments were performed on two cells with different electrode areas during low and high c-rate operation. The measurements show how charging straightened the cell layers that were slightly curved by handling of the pouch cell during setup of the experiment. Subsequent high charging rate, that exceeded the suggested operating voltage limits, was shown to have a strong influence on the observed expansion. Specifically, during high-rate cycling, the battery showed a much larger and irreversible expansion of around 1.5% which was correlated with a 4% loss in capacity over 21 cycles.
Molecular Analysis of Antigen Recognition by Insulin-Specific T-Cell Hybridomas from B6 Wild-Type and bm12 Mutant Mice (1987)

Molecular analysis of the heterodimeric T-cell antigen receptor of insulin-specific class II-restricted T-cell hybridomas (THys) derived from C57BL/6 (B6) wild-type and B6.C-H-2bm12 (bm12) mutant mice revealed that such T cells use a diverse V gene repertoire. Analysis of three THys that use related V genes, however, showed a number of novel features. Two THys that share major histocompatibility complex restriction use V alpha genes that are 98.6% homologous. Two THys sharing the same antigen fine specificity use a particular germ line V beta D beta J beta combination. A 21-base-pair deletion in the 5' segment of the J beta gene occurs in one THy, suggesting a novel mechanism for generating diversity in T-cell antigen receptor beta genes. The first amino acid encoded by N sequences at the V-D junction is conserved in a pair of T cells which recognize identical antigenic epitopes. The implications of these findings for the structural mechanisms underlying major histocompatibility complex-restricted antigen-specific T-cell recognition are discussed.
ChemInform Abstract: Regioselectivity of Methylation of O‐Demethylangolensin [1‐(2,4‐Dihydroxyphenyl)‐2‐(4‐hydroxyphenyl)propan‐1‐one]. An Expedient Synthesis of Angolensin. (2001)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Co-immunoprecipitation and semi-quantitative immunoblotting for the analysis of protein-protein interactions (2021)

Co-immunoprecipitation (co-IP) of protein complexes from cell lysates is widely used to study protein-protein interactions. However, establishing robust co-IP assays often involves considerable optimization. Moreover, co-IP results are frequently presented in non-quantitative ways. This protocol presents an optimized co-IP workflow with an analysis based on semi-quantitative immunoblot densitometry to increase reliability and reproducibility. For complete details on the use and execution of this protocol, please refer to Burckhardt et al. (2021).
Failure Analysis of InGaN/GaN High Power Light-Emitting Diodes Fabricated with ITO Transparent <I>p</I>-Type Electrode During Accelerated Electro-Thermal Stress (2012)

We have investigated the high-temperature degradation of optical power as well as electrical properties of InGaN/GaN light-emitting diodes (LEDs) fabricated with ITO transparent p-electrode during accelerated electro-thermal stress. As the thermal stress increased from 150 degrees C to 250 degrees C at a electrical stress of 200 mA, the optical power of the LEDs was significantly reduced. Degradation of the optical power was thermally activated, with the activation of 0.9 eV. In addition, the activation energy of the degradation of optical power was fairly similar to that of the degradation of series resistance of the LEDs, 1.0 eV, which implies that the increase in the series resistance may result in the severe degradation of optical power. We also showed that the increase in the series resistance of the LEDs during the accelerated electro-thermal stress can be attributed to reduction of the active acceptor concentration in the p-type semiconductor layers and local joule heating due to the current crowding.