gebhart/patrepeval huggingface dataset:
Immobilization of synthetically useful enzymes by condensation polymerization (1978)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTImmobilization of synthetically useful enzymes by condensation polymerizationAlfred Pollak, Richard L. Baughn, Orn Adalsteinsson, and George M. WhitesidesCite this: J. Am. Chem. Soc. 1978, 100, 1, 302–304Publication Date (Print):January 1, 1978Publication History Published online1 May 2002Published inissue 1 January 1978https://pubs.acs.org/doi/10.1021/ja00469a062https://doi.org/10.1021/ja00469a062research-articleACS PublicationsRequest reuse permissionsArticle Views138Altmetric-Citations20LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Paired heavy- and light-chain signatures contribute to potent SARS-CoV-2 neutralization in public antibody responses (2021)

Understanding mechanisms of protective antibody recognition can inform vaccine and therapeutic strategies against SARS-CoV-2. We report a monoclonal antibody, 910-30, targeting the SARS-CoV-2 receptor-binding site for ACE2 as a member of a public antibody response encoded by IGHV3-53/IGHV3-66 genes. Sequence and structural analyses of 910-30 and related antibodies explore how class recognition features correlate with SARS-CoV-2 neutralization. Cryo-EM structures of 910-30 bound to the SARS-CoV-2 spike trimer reveal binding interactions and its ability to disassemble spike. Despite heavy-chain sequence similarity, biophysical analyses of IGHV3-53/3-66-encoded antibodies highlight the importance of native heavy:light pairings for ACE2-binding competition and SARS-CoV-2 neutralization. We develop paired heavy:light class sequence signatures and determine antibody precursor prevalence to be ∼1 in 44,000 human B cells, consistent with public antibody identification in several convalescent COVID-19 patients. These class signatures reveal genetic, structural, and functional immune features that are helpful in accelerating antibody-based medical interventions for SARS-CoV-2.
Quantification of steroid glycosides from Hoodia gordonii in porcine plasma using high performance liquid chromatography–mass spectrometry (2011)

An HPLC–ESI–MS/MS method using collision induced dissociation – multiple reaction monitoring was developed for the quantification of eight Hoodia gordonii steroid glycosides and their metabolites in porcine plasma samples. The method was validated for the three most important glycosides and was successfully applied also for the related glycosides and metabolites. The limits of quantification were 0.04ngml⁻¹ for the two main steroid glycosides and 0.1ngml⁻¹ for the detiglated metabolites. These limits are sufficiently low to allow monitoring the concentration–time profiles in plasma after feeding H. gordonii. The standard deviations of the intra-day measurements were better than 20% for concentrations below 5ngml⁻¹ and better than 10% for concentrations above 5ngml⁻¹. The method was successfully applied to plasma samples collected from a porcine pharmacokinetics study.
High-Dielectric Polymer Composite Materials from a Series of Mixed-Metal Phenylphosphonates, ATi(C6H5PO3)3 for Dielectric Energy Storage (2010)

We report the preparation of new polymer composite dielectric materials for energy storage applications. New layered 1:1 mixed A+2/Ti4+ metal phenylphosphonates, ATi(O3PC6H5)3, A=Mg, Ca, Sr, Ba, and Pb, have been prepared via a melt route, in which mixed metal oxides, ATiO3, were reacted with molten phenyl phosphonic acid. The mixed-metal phosphonates were combined with polystyrene (PS) via a solution route and cast as thin films for dielectric permittivity measurements. The ATi(O3PC6H5)3-PS composites exhibit a substantial enhancement in the dielectric permittivity as a function of weight loading relative to the parent ATiO3-PS composites. For both ATiO3-PS and ATi(O3PC6H5)3-PS, the composites' dielectric permittivity increases with A cation polarizability. Unusually large increases for 40 wt% ATi(O3PC6H5)3-PS composites (A=Sr, Ba, and Pb) indicate permittivity enhancement that goes beyond the effect of varying filler composition.
Author response: Comparative genetic screens in human cells reveal new regulatory mechanisms in WNT signaling (2016)

When an embryo is developing, its cells must communicate with one another to coordinate the processes that shape the body’s tissues and organs. Cells often communicate by releasing signaling molecules that engage with proteins called receptors on the surface of other cells. This triggers a series of events that sends the signal along a “pathway” of biochemical reactions inside the receiving cell and leads to the activation of genes. One such signaling pathway is triggered by the WNT proteins and is used extensively in all animals. The WNT pathway instructs cells to grow and divide, establishes the identity of specific cell types and maintains populations of stem cells that can regenerate tissues in adulthood as well. The WNT pathway must be carefully regulated because various types of cancer can develop if the pathway becomes too active. Some signaling pathways are well conserved between different animals. Many genetic studies into the WNT pathway have focused on animals that are easier to work with in the laboratory, like worms or flies. However, there may be differences in the way these pathways are regulated between these model animals and humans. Therefore, to understand how the WNT pathway operates in humans, it was important to study it in human cells too. Lebensohn et al. have now carried out a series of genetic screens in human cells that contain only one copy of each gene instead of the usual two. These cells – referred to as haploid cells – are ideal for genetic studies because only a single copy of a gene has to be disrupted in order to analyze the consequences of that gene’s loss. The screens searched for genes that regulate WNT signaling: those that keep the pathway “off” in the absence of WNT and those that turn the pathway “on” in response to WNT. By comparing the outcomes of these screens, Lebensohn et al. identified previously unknown regulators and uncovered new roles for known regulators of the WNT pathway. For instance, a regulator called TFAP4, which had not previously been linked to the pathway, was shown to activate WNT signaling. In another case, enzymes that make molecules called glycophosphatidylinositol anchors, and cell-surface proteins that are modified with those anchors, were found to amplify WNT signaling. Lebensohn et al. also identified genes that were needed to sustain the uncontrolled WNT signaling in cells that carried cancer-causing mutations in this pathway. Further studies could now explore if drugs can target these genes, or the molecules encoded by them, to treat cancers in which the WNT pathway is excessively activated. Other studies could also use the same methods to explore more signaling pathways and gain new insights into important biological processes in human cells.
Failure and Damage of Polymers (2012)

No AccessMaterials Science of Polymers for EngineersSep 2012Failure and Damage of PolymersTim A. Osswald, Georg MengesTim A. OsswaldSearch for more papers by this author, Georg MengesSearch for more papers by this authorhttps://doi.org/10.3139/9781569905241.010SectionsAboutPDF ToolsAdd to FavoritesDownload CitationTrack CitationsCopy LTI LinkPDF key 'share (en)' returned an object instead of string.FacebookTwitterEmailLinkedIn previous chapternext chapter FiguresReferencesRelatedDetails 2012Pages: 423-487Print ISBN: 978-1-56990-514-2eISBN: 978-1-56990-524-1 Copyright & Permissions© 2012 Carl Hanser Verlag GmbH & Co. KGPDF DownloadLoading ...
Evaluation of commercial electroless nickel chemicals for a low cost wafer bumping process (2002)

10.1088/0268-1242/17/9/302
Development of Calorie Restriction Mimetics as a Prolongevity Strategy (2004)

By applying calorie restriction (CR) at 30-50% below ad libitum levels, studies in numerous species have reported increased life span, reduced incidence and delayed onset of age-related diseases, improved stress resistance, and decelerated functional decline. Whether this nutritional intervention is relevant to human aging remains to be determined; however, evidence emerging from CR studies in nonhuman primates suggests that response to CR in primates parallels that observed in rodents. To evaluate CR effects in humans, clinical trials have been initiated. Even if evidence could substantiate CR as an effective antiaging strategy for humans, application of this intervention would be problematic due to the degree and length of restriction required. To meet this challenge for potential application of CR, new research to create "caloric restriction mimetics" has emerged. This strategy focuses on identifying compounds that mimic CR effects by targeting metabolic and stress response pathways affected by CR, but without actually restricting caloric intake. Microarray studies show that gene expression profiles of key enzymes in glucose (energy) handling pathways are modified by CR. Drugs that inhibit glycolysis (2-deoxyglucose) or enhance insulin action (metformin) are being assessed as CR mimetics. Promising results have emerged from initial studies regarding physiological responses indicative of CR (reduced body temperature and plasma insulin) as well as protection against neurotoxicity, enhanced dopamine action, and upregulated brain-derived neurotrophic factor. Further life span analyses in addition to expanded toxicity studies must be completed to assess the potential of any CR mimetic, but this strategy now appears to offer a very promising and expanding research field.
Particle/cell manipulation and sorting with surface acoustic waves in a microfluidic device (2016)

Microfluidic manipulation technology based on surface acoustic waves (SAWs) has great potential for improving biomedical and clinical practices including drug screening, sorting, and isolation of rare cells on a single-cell level via an induced acoustic force with high biocompatibility. However, previous works on the manipulation of particles/cells using acoustic methods primarily base on their intrinsic characteristics. Characteristics such as size and acoustic contrast offer low versatility and flexibility in applications for comprehensive sample treatments. Here we investigated the mechanical properties of particles and cells in response to SAWs in a microfluidic device, in which particles/cells deflect away from the main stream. In addition, the manipulation of water droplets in oil phase was also demonstrated, extending the fields of our interests to droplet microfluidics. We expect the results to be useful for high-throughput cell sorting in various biomedical-analytical settings.
Biochemical Systematics of Populations of Peromyscus boylii. I. Populations from East-Central Mexico with Low Fundamental Numbers (1986)

Journal Article Biochemical Systematics of Populations of Peromyscus boylii. I. Populations from East-Central Mexico with Low Fundamental Numbers Get access Paul D. Rennert, Paul D. Rennert Department of Zoology, University of Vermont, Burlington, VT 05405 Search for other works by this author on: Oxford Academic Google Scholar C. William Kilpatrick C. William Kilpatrick Department of Zoology, University of Vermont, Burlington, VT 05405 Search for other works by this author on: Oxford Academic Google Scholar Journal of Mammalogy, Volume 67, Issue 3, 8 August 1986, Pages 481–488, https://doi.org/10.2307/1381279 Published: 08 August 1986 Article history Received: 04 March 1985 Accepted: 24 October 1985 Published: 08 August 1986
Supplementary Figure Legend from Downregulation of HER3 by a Novel Antisense Oligonucleotide, EZN-3920, Improves the Antitumor Activity of EGFR and HER2 Tyrosine Kinase Inhibitors in Animal Models (2023)

&lt;p&gt;Supplementary Figure Legend PDF file - 85K, Figure legend for each supplementary figure.&lt;/p&gt;
A generalized mathematical model for vector inversion generators (2008)

This paper presents a generalized mathematical model for a vector inversion generator, through which semi-analytical solutions for component stresses and output characteristics can be obtained. The derivation of a simplified equivalent circuit for transient analysis of the converter is presented in detail. The equivalent circuit model is used to analyze the performance of a 2-stage and 6-stage vector inversion generator. The analytical expressions for component stresses and output characteristics of these converters are derived in the frequency domain and transformed into the time-domain numerically to obtain a final solution. The results are presented and compared with simulated results to validate the model.
Movable micromechanical joints for use in micromechatronics (2003)

This paper deals with single-crystal silicon as a structural material. Its material properties are compared to those of other materials conventionally used for mechanical applications. The advantages; of silicon, e.g. no creep fatigue, are depicted and the most popular micromechanical techniques are named and explained. It is shown how compliant structures made from single-crystal silicon structured by wet chemical etching can be used for applications like flexible guides which allow deflections within the millimetre-range (scaling up). On the other hand, conventional principles of operation know from precision engineering can be shrunken to micromechanical dimensions. As an example, a step-by-step switchgear made by using the Silicon-On-Insulator (SOI) technology is presented.
Access to Highly Substituted 7-Azaindoles from 2-Fluoropyridines via 7-Azaindoline Intermediates (2015)

A versatile synthesis of 7-azaindoles from substituted 2-fluoropyridines is described. C3-metalation and 1,4-addition to nitroolefins provide substituted 2-fluoro-3-(2-nitroethyl)pyridines. A facile oxidative Nef reaction/reductive amination/intramolecular SNAr sequence furnishes 7-azaindolines. Finally, optional regioselective electrophilic C5-substitution (e.g., bromination or nitration) and subsequent in situ oxidation delivers highly functionalized 7-azaindoles in high overall efficiency.
Investigations on the Structural, Morphological, Electrical, and Magnetic Properties of CuFe2O4−NiO Nanocomposites (2007)

CuFe2O4−xNiO (x = 1, 5, 10, and 20 wt %) nanocomposites have been successfully prepared by a simple combustion method using urea−nitrate precursors. The samples were sintered at different temperatures, namely 600, 800, 1000, and 1100 °C, for 5 h to enhance the compound formation and phase purity, studied by means of XRD patterns. Then the 1100 °C sintered sample was further characterized for its structural (EXAFS, XANES, FT-IR, UV–vis), morphological (SEM, TEM, HRTEM, SAED), electrical (ac conductivity, dielectric constant, dielectric loss tangent), and magnetic (Mössbauer) properties. The EXAFS and XANES studies reveal the formation of NiFe2O4 and CuO, in addition to the existence of CuFe2O4 and NiO phases. A partial substitution of metal cations by nickel ions could also be evidenced. The stretching and bending vibration of the tetrahedral and octahedral complexes have been established from FT-IR spectra. The UV–vis spectra elucidate that the prepared materials are semiconductors and also show the quantum size confinement effect. The well-defined grain and grain boundary structure was identified from the SEM studies. The nanosize of the synthesized materials has been identified by TEM investigations. The HRTEM and SAED images reveal the crystallinity and polycrystalline behavior of the as-synthesized materials. The electrical studies show the normal ferrimagnetic behavior of the materials. The inverse spinel nature of the materials has been enumerated from Mössbauer spectra, which also illustrates the phase transition behavior.
Fractionation of Single Wall Carbon Nanotubes by Length Using Cross Flow Filtration Method (2010)

A novel system for fractionating single wall carbon nanotubes (SWCNTs) by length via a three-step cross-flow filtration has been developed in which three membrane filters of different pore sizes, 1.0, 0.45, and 0.2 microm, were used. SWCNTs dispersed in water with the help of sodium carboxymethylcellulose (CMC) detergents were successfully sorted into four samples, and the atomic force microscopy (AFM) observation of those samples confirmed that their length distribution peaks are within the expected ranges from pore sizes of used filters. However, the result of the similar filtration process using a different detergent, sodium dodecylbenzenesulfonate (SDBS), showed no pronounced correlation between the length distribution of SWCNTs and the pore size. The observed difference in the sorting phenomena caused by the detergent type suggests that the permeation property depends on the complex structure resulting from the dispersed SWCNTs and detergent molecules.
Dynamic adaptation of a vehicle's cruising speed with recurrent neural networks for enhancing fuel economy (2013)

This paper presents an architecture for optimally modulating the cruise speed around its set point. Our objective is to minimize the overall fuel consumption over a trip without impacting the overall trip time, by exploiting the vehicle dynamics and the terrain, specifically in this paper, the road grades. The overall trip time is defined as the time to complete the trip while driving at the constant cruise speed, which was set by the driver at the beginning of the trip. We test this architecture with data acquired by an instrumented vehicle driven on city and highway roads in Southeast Michigan. Our testing was very promising and showed that we can achieve up to 11% of overall fuel economy of which 10.8% are from exploiting the road grades.
LCC Zero-Voltage-Switching Buck Converter with Synchronous Rectifier (2006)

LCC cell is proposed in this paper to achieve zero-voltage-switching (ZVS) and eliminate body-diode conduction and reverse recovery in buck converter with synchronous rectifiers (SRs). In low-voltage dc-dc converters, SRs are widely utilized to reduce rectifier conduction loss. However, during switches' transition, SRs' parasitic body diodes unavoidably carry load current, which result in extra body diode conduction loss. In addition, body diodes' reverse recovery leads to significant switching losses and electromagnetic interference (EMI) problem, which is the key obstacle for SR applications for high-voltage and high switching frequency. With the proposed LCC cell in buck converter, the SR body diode never involves during switching transition, therefore, body diode conduction loss is saved, and reverse-recovery-related switching loss and ringing are eliminated. The experimental results show that the proposed converter achieves significant improvement in efficiency running at 1MHz switching frequency compared to the conventional buck converter and QSW buck converter
Hall Thruster Cathode Flow Impact on Coupling Voltage and Cathode Life (2012)

The cathode coupling voltage in Hall thrusters, which is the voltage difference between the cathode and the thruster beam plasma potential, is considered an indicator of the ease with which electrons flow from cathode to anode.Historically, the coupling voltage has beenminimizedby increasing the amount of propellant injected through the hollow cathode due to early observations that thismaximizes the discharge (or anode) efficiency.However, recent experiments described here show that the total thruster efficiency is independent of the cathode flow over the range from 5 to 10%of the propellant injected into the thruster body through the anode. For this reason, cathode flow rates can be reduced closer to the classic plume mode limit characteristic of the hollow cathode design without impacting the total thruster efficiency. Such reductions in cathode flow rate can significantly extend the cathode life, especially for higher-power Hall thrusters with larger discharge currents, where the normal Hall thruster cathode flow split will significantly exceed the optimum level for cathode operation and life.
Area and Power Efficient Trellis Computational Blocks in 0.13μm CMOS (2005)

Improved add-compare-select and branch metric units are presented to reduce the complexity in the implementation of trellis-based decoding architectures. These units use a complementary property of the best rate 1/2 convolutional codes to reduce both area requirements and power consumption in a silicon implementation with no loss in decoding performance. For a 0.13/spl mu/m CMOS process, synthesized computational blocks for decoders that can process codes from memory 2 up to 7 show up to 17% savings in both cell area and power consumption.
ChemInform Abstract: Studies in Sugar Chemistry. Part 7. Glucuronides of Podophyllum Derivatives (1998)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Experimental verification of an angle-sensorless control scheme for bearingless permanent magnet machines (2017)

Bearingless machines are used for a variety of applications with demand for low mechanical loss, low wear and low contamination. These machines use contact-free magnetic suspension to levitate the rotor. The control of the machine requires precise radial and angular position information in order to ensure stable levitation. This information is usually obtained with two types of sensors: radial displacement sensors and angle sensors. Alternatively, an angle-sensorless control scheme can be used, reducing the complexity and the cost of the machine. While such a control is well known for conventional machines it is challenging to adapt it for bearingless machines. The reason is that most methods fail to provide the angle information at zero and at low speed but bearingless machines require knowledge about the rotor angle at all speeds in order to function. The theoretical mode of operation of a model based angle observer for zero and low speed operation of a bearingless machine was shown in previous publications. The observer obtains the rotor angle estimation error by analyzing the performance of the radial bearing and comparing it to the performance of a model with zero angle error. This observer can be used for operation at standstill and over the whole speed range. This paper provides a more detailed description of the non-idealities of the zero and low speed observer and presents results of machine operation without angle sensors. The generation of torque and force inside the machine is analyzed in more detail. Furthermore, it is shown how to combine the novel observer with a conventional, back electromotive force based, high speed angle observer. The experimentally verified results of this paper indicate that the novel observer can be used up to speeds at which back electromotive force estimation is possible. This allows the efficient, angle-sensorless operation of the machine over the whole speed range.