gebhart/patrepeval huggingface dataset:
A COMBINED JOINT DIAGONALIZATION-MUSIC ALGORITHM FOR ESTIMATING LOCATIONS OF SUBSURFACE TARGETS (2014)

In this paper we present a method for extracting target location from data measured by Time-Domain Electromagnetic Multisensor Towed Array Detection System (TEMTADS). The TEMTADS is consisted of square-loop transmitters and 3-D receivers, arranged in a 2x2 array, which generates multistatic response (MSR) matrices of 4×12 in 52 time channels. The data collected first goes through a linear combination process to form a square matrix so we can apply joint diagonalization (JD), a technique that finds the eigenvectors which diagonalize the entire set of MSR matrices. A filtering process is embedded in the JD to enhance signal-to-noise ratio (SNR). The eigenvectors arising from targets of interest (TOI) and from noise can be identified after applying JD, and this information is passed to a multiple signal classification (MUSIC) algorithm to separate the signal and noise subspaces. MUSIC algorithm then projects the noise subspace onto a theoretically calculated Green’s Function array. Due to their orthogonality, the target locations can be estimated by looking for maximums when we invert this multiplication. With the Green’s Function pre-calculated, the method can be carried out fast enough to perform targets mapping in real or near real-time.
Methanol and Humidity Capacitive Sensors Based on Thin Films of MOF Nanoparticles (2020)

The successful development of modern gas sensing technologies requires high sensitivity and selectivity coupled to cost effectiveness, which implies the necessity to miniaturize devices while reducing the amount of sensing material. The appealing alternative of integrating nanoparticles of a porous metal-organic framework (MOF) onto capacitive sensors based on interdigitated electrode (IDE) chips is presented. We report the deposition of MIL-96(Al) MOF thin films via the Langmuir-Blodgett (LB) method on the IDE chips, which allowed the study of their gas/vapor sensing properties. First, sorption studies of several organic vapors like methanol, toluene, chloroform, etc. were conducted on bulk MOF. The sorption data revealed that MIL-96(Al) presents high affinity toward water and methanol. Later on, ordered LB monolayer films of MIL-96(Al) particles of ∼200 nm were successfully deposited onto IDE chips with homogeneous coverage of the surface in comparison to conventional thin film fabrication techniques such as drop-casting. The sensing tests showed that MOF LB films were selective for water and methanol, and short response/recovery times were achieved. Finally, chemical vapor deposition (CVD) of a porous thin film of Parylene C (thickness ∼250-300 nm) was performed on top of the MOF LB films to fabricate a thin selective layer. The sensing results showed an increase in the water selectivity and sensitivity, while those of methanol showed a huge decrease. These results prove the feasibility of the LB technique for the fabrication of ordered MOF thin films onto IDE chips using very small MOF quantities.
Substituted Indole-2-carboxylates as in Vivo Potent Antagonists Acting as the Strychnine-Insensitive Glycine Binding Site (1997)

A series of indole-2-carboxylates bearing suitable chains at the C-3 position of the indole nucleus was synthesized and evaluated in terms of in vitro affinity using [3H]glycine binding assay and in vivo potency by inhibition of convulsions induced by N-methyl-D-aspartate (NMDA) in mice. 3-[2-[(Phenylamino)carbonyl]ethenyl]-4,6-dichloroindole-2-carboxyl ic acid (8) was an antagonist at the strychnine-insensitive glycine binding site (noncompetitive inhibition of the binding of [3H]TCP, pA2 = 8.1) displaying nanomolar affinity for the glycine binding site (pKi = 8.5), coupled with high glutamate receptor selectivity (> 1000-fold relative to the affinity at the NMDA, AMPA, and kainate binding sites). This indole derivative inhibited convulsions induced by NMDA in mice, when administered by both iv and po routes (ED50 = 0.06 and 6 mg/kg, respectively). The effect of the substituents on the terminal phenyl ring of the C-3 side chain was investigated. QSAR analysis suggested that the pKi value decreases with lipophilicity and steric bulk of substituents and increases with the electron donor resonance effect of the groups present in the para position of the terminal phenyl ring. According to these results the terminal phenyl ring of the C-3 side chain should lie in a nonhydrophobic pocket of limited size, refining the proposed pharmacophore model of the glycine binding site associated with the NMDA receptor.
All That Glitters Is Not Gold: 3D-Printed Tactile Image Configurations for Better Visually Impaired People’s Perception (2023)

This research focused on the perception of visually impaired individuals regarding tactile image creation using 3D printing. We developed and applied an experiment with 28 participants with visual impairments. They performed tasks based on skills whose foundations are needed and used in their daily lives, such as scanning, tactile discrimination, picture and spatial comprehension. The tactile images used in this study were developed by additive manufacturing and printed over 180 g/m² sulphite paper. The best performances were obtained when (1) the tactile image contour line heights were between 0.2 mm and 0.4 mm; and (2) the texture presented densities between 10% and 50%, when alone, or between 10% and 90%, when combined with another texture. The tested line widths (0.8 mm and 1.2 mm) did not present statistically significant differences in the results. Vision status (low vision or blindness) and moment of vision loss (congenital or late blind) did not influence the results. Schooling affected the activities of identifying objects and locating their copies. In the Conclusion section, there is a detailed recommendation list for the production and application of tactile materials, which range from simplifying forms to encouraging the use of both hands during content assimilation.
Improved standardization methods for clinical measurements of BUA and SOS (2008)

BACKGROUND: Although calcaneal broadband ultrasound attenuation (BUA) and speed of sound (SOS) are good predictors of osteoporotic fracture risk, BUA and SOS measurements exhibit substantial inter-system variability. PURPOSE: 1) Compare phase insensitive (PI) detection, which suppresses phase cancellation, and conventional phase sensitive (PS) detection for measurement of BUA. 2) Test a new compensation formula for reducing variability in SOS measurements. METHODS: Data from 73 women were acquired using a GE Lunar Achilles Insight bone sonometer. Radio frequency data were processed off-line using both PI and PS algorithms. RESULTS: BUA measurements (mean ± sd) were 81.4 ± 21.4 dB/MHz (PS) and 67.2 ± 9.7 dB/MHz (PI). Compensation of SOS measurements reduced 1) average transit-time-marker-related SOS variability by 75% in 73 women and 2) bandwidth-related SOS variability by 80% in a bone-mimicking phantom. CONCLUSION: These new methods will enable a substantial improvement in consistency in bone sonometry. The mention of commercial products, their sources, or their use in connection with material reported herein is not to be construed as either an actual or implied endorsement of such products by the Department of Health and Human Services.
LH rise on the day of GnRH antagonist commencement (2015)

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PSMA-Specific CAR-Engineered T Cells for Prostate Cancer: CD28 Outperforms Combined CD28-4-1BB “Super-Stimulation” (2021)

Prostate cancer (PCa) is the second leading cause of malignancy-related mortality in males in the Western world. Although treatment like prostatectomy and radiotherapy for localized cancer have good results, similar positive outcomes are not achieved in metastatic PCa. Consequently, these aggressive and metastatic forms of PCa urgently need new methods of treatment. We already described an efficient and specific second-generation (2G) Chimeric Antigen Receptor (CAR) against Prostate Specific Membrane Antigen (PSMA), a glycoprotein overexpressed in prostate cancer and also present on neovasculature of several tumor entities. In an attempt to improve efficacy and <i>in vivo</i> survival of anti-PSMA 2G CAR-T cells, we developed a third generation (3G) CAR containing two costimulatory elements, namely CD28 and 4-1BB co-signaling domains, in addition to CD3ζ. Differently from what described for other 3G receptors, our third generation CAR disclosed an antitumor activity <i>in vitro</i> similar to the related 2G CAR that comprises the CD28 co-signaling domain only. Moreover, the additional costimulatory domain produced detrimental effects, which could be attributed to an increased activation-induced cell death (AICD). Indeed, such "superstimulation" resulted in an exhausted phenotype of CAR-T cells, after prolonged <i>in vitro</i> restimulation, a higher frequency of cell death, and an impairment in yielding sufficient numbers of transgenic T lymphocytes. Thus, the optimal combination of costimulatory domains for CAR development should be assessed cautiously and evaluated case-by-case.
HFN-Based Right Management for IoT Health Data Sharing (2021)

블록체인 기술이 IoT를 위한 보안 이슈로 대두됨에 따라, IoT에 블록체인을 통합하는 기술이 연구되고 있다. 본 논문은 IoT 데이터 공유를 위한 토큰 기반 IoT 서비스 접근 제어 기술에 관한 연구로 허가형 블록체인을 이용하여 소유자 중심의 데이터 공유 기술을 제안한다. IoT health 데이터를 공유하고자 서로 다른 서비스에 대해 디바이스 소유자 중심의 각각 다른 접근제어 정책을 적용하여 데이터를 공유하기 위한 방법을 제공하기 위해 3개의 조직으로 구성된 하이퍼레저 패브릭 네트워크(HFN, Hyperledger Fabric Network)를 설계하였다. 제안하는 시스템에서 디바이스 소유자는 조직의 참여자에 대해 서로 다른 보안 레벨이 적용된 권한 토큰을 발행하며, 토큰 발행 정보는 HFN의 분산 원장을 통해 공유된다. IoT에서 데이터에 접근을 요청하는 서비스 요청자들에게 토큰을 부여함으로써, IoT 장치들의 접근제어 처리를 경량화할 수 있다. 또한, HFN을 이용하여 네트워크 참여자간에 토큰 발행 정보를 공유함으로서 토큰에 대한 무결성을 보장하고 모든 네트워크 참여자들이 토큰을 신뢰할 수 있다. 디바이스 소유자는 자신의 데이터가 허가된 권한 안에서 사용되고 있음을 신뢰할 수 있고, 데이터 수집 및 사용에 대해 제어할 수 있다.
Preparation and characterization of poly(ethylene 2,5-furandicarboxylate/nanocrystalline cellulose composites via solvent casting (2017)

Abstract The effect of nanocrystalline cellulose dispersion on the nonisothermal crystallization of poly(ethylene 2,5-furandicarboxylate) (PEF) has been investigated by means of solvent casting. The cellulose dispersion plays a significant role on the crystallization temperature, thus dispersive equipments of increasing energies were employed to improve the cellulose particles disaggregation. Therefore, ultra-sonic bath, ultra-sonication, and ultra-turrax were used to disperse cellulose nanocrystals in 1,1,1,3,3,3-hexafluoro-2-propanol. Dissolved separately in the same solvent, PEF was then poured into the cellulose suspension before casting. The cellulose whiskers were inspected by transmission electron microscopy. Differential scanning calorimetry was used to measure the crystallization temperature, while scanning electron microscopy visualized the cellulose dispersion at the fracture surface. After investigation on the interaction of cellulose/PEF via Fourier transform infrared spectroscopy, the thermal stability of the blends was measured by means of thermogravimetric analysis.
The art of back-of-the-envelope paraxial raytracing (2001)

Paraxial raytracing is a valuable tool for making quick "back-of-the-envelope" calculations in optical system design. Its popularity has been highly diminished as a consequence of the growing sophistication of computer-aided raytracing and the availability of powerful computers. The availability of raytracing computer programs does not harm in itself the usefulness of graphical raytracing, but it makes people forget, or never learn the basics of graphical raytracing. In this paper, some of the basic concepts of graphical raytracing are presented, along with some examples that illustrate how it can be applied to multielement systems. The mastering of the methods presented in this paper constitute some of the fundamentals required in the use of any optical software. Such knowledge is absolutely necessary for the conception of novel optical systems.
Continuous monitoring of IgG using immobilized fluorescent reporters (2022)

In the manufacture of therapeutic monoclonal antibodies, the clarified cell culture fluid (CCF) is typically loaded onto an initial protein A affinity capture column. Imperfect mass transfer and loading to maximum capacity can risk antibody breakthrough and loss of valuable product, but conservative underloading wastes expensive protein A resin. In addition, the effects of column fouling and ligand degradation require the frequent optimization of immunoglobulin G (IgG) loading to avoid wastage. Continuous real-time monitoring of IgG flowthrough is of great interest, therefore. We previously developed a fluorescence-based monitoring technology that allows batch mix-and-read mAb detection in the CCF. Here, we report the use of reporters immobilized on cyanogenbromide-activated Sepharose 4B resin for continuous detection of IgG in column breakthrough. The column effluent is continuously contacted with immobilized fluorescein-labeled Fc-binding ligands in a small monitoring column to produce an immediately-detectable change in fluorescence intensity. The technology allows rapid and reliable monitoring of IgG in a flowing stream of clarified CCF emerging from a protein A column, without prior sample preparation. We observed a significant change in fluorescence intensity at 0.5 g/L human IgG, sufficient to detect a 5% breakthrough of a 10 g/L load, within 18 s at a flow rate of 0.5 ml/min. The current small-scale technology is suitable for use in process development, but the chemistry should be readily adaptable to larger scale applications using fiber-optic sensors, and continuous IgG monitoring could be applicable in a variety of upstream and downstream process settings.
Tumorigenicity of simian virus 40-transformed human cells and mouse--human hybrids in nude mice. (1977)

Four different human cell lines transformed by simian virus 40 (SV40) were tested for their tumorigenicity in athymic nude mice. Two of these lines, W18Va2 and GM52VA, were found to be tumorigenic when inoculated at a concentration of 1 x 10(7) cells per mouse. The other two cell lines, LN-SV and GM54VA, were found to induce very small tumors only after the injection of approximately 1 x 10(8) cells per mouse. Somatic cell hybrids between either LN-SV or GM54VA SV40-transformed human cells and normal mouse peritoneal macrophages, which have retained the human chromosomes carrying the SV40 genome, were found to be much more tumorigenic than the SV40-transformed human cell parents. These experiments suggest that the genetic background in which the human chromosomes carrying the SV40 genome are present plays a role in the modulation of the expiration of malignancy.
DC distribution system architecture and controls for wind power applications (2012)

In this paper, Medium Voltage DC (MVDC) distribution system is proposed for wind power applications. The proposed MVDC system is favorable for off shore wind farms. The architecture of the system, control for the medium voltage three phase inverter, active and reactive power control for voltage and frequency support are discussed in the paper. The proposed topology allows for easier integration of energy storage with wind energy. The simulation results show the viability of the proposed system for active/reactive power controls for wind farms.
Correction Factors for Estimating Potassium Concentrations in Samples With In Vitro Hemolysis: A Detriment to Patient Safety (2009)

Abstract Context. —Correction factors have been proposed for estimating true potassium concentrations in blood samples with evidence of in vitro hemolysis. Objective. —We used 2 different models of true (ie, nonsimulated) in vitro hemolysis to evaluate the clinical utility of correction factors for estimating potassium concentrations in samples with evidence of in vitro hemolysis. Design. —Potassium correction factors were derived using 2 different models. In model 1, potassium and plasma hemoglobin were measured with the Hitachi 747 analyzer in 132 paired blood samples, with each pair consisting of 1 sample with evidence of hemolysis and 1 without, collected during the same phlebotomy procedure. The change in measured potassium concentration was plotted versus the change in plasma hemoglobin concentration for each pair of samples. In model 2, the potassium levels of 142 784 blood samples and the corresponding hemolytic index values were measured with the Beckman LX20 analyzer. Potassium concentrations at the 10th, 25th, 50th, 75th, and 90th percentiles were calculated for each hemolysis index category. Results. —From our 2 models, we derived correction factors expressing an increase in potassium concentration of 0.51 and 0.40 mEq/L for every increase in plasma hemoglobin concentration of 0.1 g/dL. These correction factors are much higher than those reported in studies that simulated in vitro hemolysis by freeze-thaw lysis or osmotic disruption of whole blood. Conclusions. —Use of correction factors for estimating the true potassium concentration in samples with evidence of in vitro hemolysis is not recommended. Derivation of correction factors by using samples with nonsimulated in vitro hemolysis suggests that the actual increase in potassium in hemolyzed samples is much higher than that reported in previous studies that produced hemolysis with artificial means.
Research Note: Isolation, speciation, and anticoccidial sensitivity of Eimeria spp. recovered from wild turkey feces in the United States (2023)

Between 2018 and 2020, over 100 wild turkey fecal samples were collected from the Eastern and Central thirds of the United States, where commercial turkey production is uncommon. We hypothesized that anticoccidial-sensitive Eimeria spp. would be present in wild turkey fecal samples. Samples containing Eimeria spp. oocysts were amplified in vivo. If propagation was successful, the samples were PCR-speciated and subjected to anticoccidial sensitivity testing (AST) for key members of both ionophore and chemical categories of anticoccidial drugs. The purpose of this study was to isolate Eimeria spp. relevant to commercial turkey production that possessed sensitivity to monensin, zoalene, and amprolium. Future research would evaluate the efficacy of wild turkey Eimeria spp. as vaccine candidates for reducing coccidiosis in commercial turkey flocks utilizing single oocyst-derived stocks obtained in the present study.
Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development (2003)

Mitochondrial morphology is determined by a dynamic equilibrium between organelle fusion and fission, but the significance of these processes in vertebrates is unknown. The mitofusins, Mfn1 and Mfn2, have been shown to affect mitochondrial morphology when overexpressed. We find that mice deficient in either Mfn1 or Mfn2 die in midgestation. However, whereas Mfn2 mutant embryos have a specific and severe disruption of the placental trophoblast giant cell layer, Mfn1-deficient giant cells are normal. Embryonic fibroblasts lacking Mfn1 or Mfn2 display distinct types of fragmented mitochondria, a phenotype we determine to be due to a severe reduction in mitochondrial fusion. Moreover, we find that Mfn1 and Mfn2 form homotypic and heterotypic complexes and show, by rescue of mutant cells, that the homotypic complexes are functional for fusion. We conclude that Mfn1 and Mfn2 have both redundant and distinct functions and act in three separate molecular complexes to promote mitochondrial fusion. Strikingly, a subset of mitochondria in mutant cells lose membrane potential. Therefore, mitochondrial fusion is essential for embryonic development, and by enabling cooperation between mitochondria, has protective effects on the mitochondrial population.
Identification of a Proliferation Gene Cluster Associated with HPV E6/E7 Expression Level and Viral DNA Load in Invasive Cervical Carcinoma (2005)

Specific HPV DNA sequences are associated with more than 90% of invasive carcinomas of the uterine cervix. Viral E6 and E7 oncogenes are key mediators in cell transformation by disrupting TP53 and RB pathways. To investigate molecular mechanisms involved in the progression of invasive cervical carcinoma, we performed a gene expression study on cases selected according to viral and clinical parameters. Using Coupled Two-Way Clustering and Sorting Points Into Neighbourhoods methods, we identified a Cervical Cancer Proliferation Cluster composed of 163 highly correlated transcripts, many of which corresponded to E2F pathway genes controlling cell proliferation, whereas no primary TP53 targets were present in this cluster. The average expression level of the genes of this cluster was higher in tumours with an early relapse than in tumours with a favourable course (P=0.026). Moreover, we found that E6/E7 mRNA expression level was positively correlated with the expression level of the cluster genes and with viral DNA load. These findings suggest that HPV E6/E7 expression level plays a key role in the progression of invasive carcinoma of the uterine cervix via the deregulation of cellular genes controlling tumour cell proliferation. HPV expression level may thus correspond to a biological marker useful for prognosis assessment and specific therapy of the disease.
Structural Mass Spectrometry of the αβ-Tubulin Dimer Supports a Revised Model of Microtubule Assembly (2009)

The molecular basis of microtubule lattice instability derives from the hydrolysis of GTP to GDP in the lattice-bound state of alphabeta-tubulin. While this has been appreciated for many years, there is ongoing debate over the molecular basis of this instability and the possible role of altered nucleotide occupancy in the induction of a conformational change in tubulin. The debate has organized around seemingly contradictory models. The allosteric model invokes nucleotide-dependent states of curvature in the free tubulin dimer, such that hydrolysis leads to pronounced bending and thus disruption of the lattice. The more recent lattice model describes a predominant role for the lattice in straightening free dimers that are curved regardless of their nucleotide state. In this model, lattice-bound GTP-tubulin provides the necessary force to straighten an incoming dimer. Interestingly, there is evidence for both models. The enduring nature of this debate stems from a lack of high-resolution data on the free dimer. In this study, we have prepared alphabeta-tubulin samples at high dilution and characterized the nature of nucleotide-induced conformational stability using bottom-up hydrogen/deuterium exchange mass spectrometry (H/DX-MS) coupled with isothermal urea denaturation experiments. These experiments were accompanied by molecular dynamics simulations of the free dimer. We demonstrate an intermediate state unique to GDP-tubulin, suggestive of the curved colchicine-stabilized structure at the intradimer interface but show that intradimer flexibility is an important property of the free dimer regardless of nucleotide occupancy. Our results indicate that the assembly properties of the free dimer may be better described on the basis of this flexibility. A blended model of assembly emerges in which free-dimer allosteric effects retain importance, in an assembly process dominated by lattice-induced effects.