gebhart/patrepeval huggingface dataset:
Cell‐surface Vimentin: A mislocalized protein for isolating csVimentin+CD133− novel stem‐like hepatocellular carcinoma cells expressing EMT markers (2014)

Recent advances in cancer stem cell biology have shown that cancer stem-like cells with epithelial-mesenchymal transition (EMT) phenotypes are more aggressive and cause relapse; however, absence of a specific marker to isolate these EMT stem-like cells hampers research in this direction. Cell surface markers have been identified for isolating cancer stem-like cells, but none has been identified for isolating cancer stem-like cells with EMT phenotype. Recently, we discovered that Vimentin, an intracellular EMT tumor cell marker, is present on the surface of colon metastatic tumor nodules in the liver. In our study, we examined the potential of targeting cell surface Vimentin (CSV) to isolate stem-like cancer cells with EMT phenotype, by using a specific CSV-binding antibody, 84-1. Using this antibody, we purified the CSV-positive, CD133-negative (csVim(+) CD133(-) ) cell population from primary liver tumor cell suspensions and characterized for stem cell properties. The results of sphere assays and staining for the stem cell markers Sox2 and Oct4A demonstrated that csVim(+) CD133(-) cells have stem-like properties similar to csVim(-) CD133(+) population. Our investigation further revealed that the csVim(+) CD133(-) cells had EMT phenotypes, as evidenced by the presence of Twist and Slug in the nucleus, the absence of EpCAM on the cell surface and basal level of expression of epithelial marker E-cadherin. The csVimentin-negative CD133-positive stem cells do not have any EMT phenotypes. csVim(+) CD133(-) cells exhibited more aggressively metastatic in livers than csVim(-) CD133(+) cells. Our findings indicate that csVim(+) CD133(-) cells are promising targets for treatment and prevention of metastatic hepatocellular carcinoma.
Osteogenic differentiation of human mesenchymal stem cells on poly(ethylene glycol)‐variant biomaterials (2008)

This study evaluated the osteogenic differentiation of human mesenchymal stem cells (MSCs), on tyrosine-derived polycarbonates copolymerized with poly(ethylene glycol) (PEG) to determine their potential as a scaffold for bone tissue engineering applications. The addition of PEG in the backbone of polycarbonates has been shown to alter mechanical properties, degradation rates, degree of protein adsorption, and subsequent cell adhesion and motility in mature cell phenotypes. Its effect on MSC behavior is unknown. MSC morphology, motility, proliferation, and osteogenic differentiation were evaluated on polycarbonates containing 0-5% PEG over a 14 day culture. MSCs on polycarbonates containing 0% or 3% PEG content upregulated the expression of osteogenic markers as demonstrated by alkaline phosphatase activity and osteocalcin expression although at different stages in the 14 day culture. Cells on polycarbonates containing no PEG were characterized as having early onset of cell spreading and osteogenic differentiation. Cells on 3% PEG surfaces were delayed in cell spreading and osteogenic differentiation, but had the highest motility as compared with cells on substrates containing no PEG and substrates containing 5% PEG at early time points. Throughout the culture, cells on polycarbonates containing 5% PEG had the lowest levels of osteogenic markers, displayed poor cell-substrate adhesion, and established cell-cell aggregates. Thus, designing substrates with minute variations in PEG may serve as a tool to guide MSC adhesion and motility accompanying osteogenic differentiation, and may be beneficial for abundant bone tissue formation in vivo.
Comparison of Fault Detection and Exclusion Algorithms for Multi-Constellation Carrier Phase Solutions (2018)

In this work, we establish the analytical model of the RANSAC-based FDE approach for a GNSS navigation solution. This approach is compared against RAIM-based FDE for differential carrier phase measurements in a multi-GNSS receiver. The capability of excluding faulty measurements will be evaluated in terms of integrity risks and protection levels. RANSAC FDE is capable of protecting the solution from multiple simultaneous faults, and has a predictable and well-constrained computational cost.
Primate-specific evolution of noncoding element insertion into PLA2G4Cand human preterm birth (2010)

Our findings suggest that variation in PLA2G4C may influence preterm birth risk by increasing levels of prostaglandins, which are known to regulate labor.
Differences in the distribution of CD34 epitopes on normal haemopoietic progenitor cells and leukaemic blast cells (1996)

The CD34 molecule expressed on haemopoietic progenitor cells contains a large number of epitopes whose expression may be related to the maturation or function of the cells. Monoclonal antibodies specific for different epitopes have been reported to detect different numbers of CD34+ leukaemic blast cells. We wanted to confirm this observation and study whether parallel findings could be observed for normal haemopoietic progenitor cells. The cells were immunophenotyped by flow cytometry with a series of monoclonal antibodies reactive with different CD34 epitopes. Class III epitopes (resistant to enzymatic cleavage with neuraminidase, chymopapain and a glycoprotease from Pasteurella haemolytica) showed a broader distribution on normal haemopoietic progenitor cells and leukaemic blast cells than class I epitopes (sensitive to cleavage with all three enzymes) and class II epitopes (sensitive to degradation with glycoprotease and chymopapain, and resistant to neuraminidase). The subpopulation of normal progenitor cells which exclusively expressed class III epitopes had flow cytometric characteristics compatible with mature myeloid progenitor cells whereas class I, II and III epitopes were equally expressed on cells enriched for immature subsets. No discordant epitope expression could be observed for the more immature leukaemias (AML-M0/1) and a higher percentage of the more mature leukaemic blast cells (AML-M3 and AML-M4/5) expressed class III epitopes compared to the percentage expressing class I and II epitopes. These data indicate that CD34 class III epitopes are more broadly distributed on normal haemopoietic progenitor cells and leukaemic blast cells than class I and II epitopes, and that class I and II epitopes may be down-regulated prior to class III epitopes during normal haemopoietic progenitor cell differentiation. These findings should be considered when selecting CD34 mabs for quantification and positive selection of haemopoietic progenitor cells for research and clinical purposes.
Evozierte Kohärenz des EEG: II. Kommunikation der Hirnareale und Verarbeitungstiefe (1996)

The in part one [42] introduced method of evoked coherences was used in search for independent signs of the levels of processing effects on memory performance. 9 subjects in a visual pilot study and 16 subjects in the acoustic main experiments were presented 30 words each in a perceptive, semantic and metacognitive orientation condition. A 16-channel-EEG was recorded and stimulus-related time-variant spectral components (δ, ϑ, α, β) were calculated for analysis of evoked coherence. In order to differentiate between task processing and general functional state of the brain in the main study one series of experimental conditions was carried out in the state of deep relaxation (trance). The evoked coherences were tested with multi-factorial ANOVA and contrasted by Sche/fe-test. As expected, the variation in the state of the consciousness was related with strong but diffuse changes in power and coherence spectra. In contrast to this, differences in level of processing were accompanied by specific focal patterns of evoked coherence with nearly no changes in power. Especially in the lower frequency bands there appeared differentiating coherences between posterior regions and single points of the frontal and left-temporal regions of the cortex. The data also supported the interpretation that „deeper processing” is characterized by a functional de-coupling of activity in progressively more anteriory located regions and within lower frequency band, particularly 5.
Cytoplasmic p27 promotes epithelial–mesenchymal transition and tumor metastasis via STAT3-mediated Twist1 upregulation (2015)

p27 restrains normal cell growth, but PI3K-dependent C-terminal phosphorylation of p27 at threonine 157 (T157) and T198 promotes cancer cell invasion. Here, we describe an oncogenic feedforward loop in which p27pT157pT198 binds Janus kinase 2 (JAK2) promoting STAT3 (signal transducer and activator of transcription 3) recruitment and activation. STAT3 induces TWIST1 to drive a p27-dependent epithelial-mesenchymal transition (EMT) and further activates AKT contributing to acquisition and maintenance of metastatic potential. p27 knockdown in highly metastatic PI3K-activated cells reduces STAT3 binding to the TWIST1 promoter, TWIST1 promoter activity and TWIST1 expression, reverts EMT and impairs metastasis, whereas activated STAT3 rescues p27 knockdown. Cell cycle-defective phosphomimetic p27T157DT198D (p27CK-DD) activates STAT3 to induce a TWIST1-dependent EMT in human mammary epithelial cells and increases breast and bladder cancer invasion and metastasis. Data support a mechanism in which PI3K-deregulated p27 binds JAK2, to drive STAT3 activation and EMT through STAT3-mediated TWIST1 induction. Furthermore, STAT3, once activated, feeds forward to further activate AKT.
Distinct patterns of minimal residual disease associated with graft-versus-host disease after allogeneic bone marrow transplantation for chronic myelogenous leukemia. (1995)

The persistence of PCR-detectable bcr-abl-positive cells early post-BMT in more than 80% of patients suggests that neither BMT preparative regimen effectively eradicates CML cells in most patients. Subsequently, acute and/or chronic GVHD are associated with a decreased ability to detect residual bcr-abl-positive cells, which suggests that immunologic mechanisms mediated by donor cells are important for inducing long-term remissions after BMT. The demonstration that 44% of patients without GVHD had either low or undetectable levels of residual leukemia suggests the presence of mechanisms capable of suppression or eradication of CML independent of GVHD.
Catheter Ablation of Chronic Atrial Fibrillation Targeting the Reinitiating Triggers (2000)

PVs are the dominant triggers reinitiating chronic AF in this patient population. Elimination of PV potentials by ostial RF applications results in stable sinus rhythm in 60%. A larger group and longer follow-up are needed to investigate further the role of trigger ablation in curative therapy for chronic AF.
Integrated Tunable Bell State Generator and Hong-Ou-Mandel Experiment on AlGaAsOI (2023)

We report an AlGaAsOI quantum photonic circuit with multiple pair sources and tunable interferometers. We demonstrate Hong-Ou-Mandel interference between two microring resonators and a tunable Bell state generator producing Ψ + and Φ − bi-photon entangled states.
Porous Organic Alloys (2012)

Another brick in the wall: Porous ternary cocrystals were prepared by chiral recognition between organic cage modules. One module, CC1, is ordered on 50 % of the lattice positions with respect to two other modules, CC3 and CC4, that are disordered across the other 50 % of sites (see picture). There is a linear relationship between relative module composition and the cocrystal lattice parameters.
An efficient particle filter for jump Markov nonlinear systems (2004)

We present an efficient particle filtering algorithm for state estimation of jump Markov nonlinear systems. The standard particle filter can easily show particle degeneracy around mode changes. In the new algorithm, the number of particles in each mode is a tuning parameter that can be selected, for instance, a priori by the designer without distorting the posterior probability density function. This new algorithm offers a more accurate estimate with the same number of particles. Although the results of this paper are generally applicable, the paper focusses on state estimation for jump Markov systems in a radar target tracking application.
Copper Hydroxide Ethanedisulfonate: A Cationic Inorganic Layered Material for High‐Capacity Anion Exchange (2011)

Cationic layers: Copper hydroxide ethanedisulfonate consists of cationic sheets (see structure of a [Cu4(OH)6]2+ layer; Cu green, O red, H white) with ethanedisulfonate as extraframework counteranion. This material shows excellent anion exchange properties for both organics and metal oxo anion pollutants, with over five times higher adsorption capacity for permanganate than hydrotalcite. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Measuring Trust in Government: A Hong Kong Perspective (2008)

Trust and legitimacy occupy a central position in contemporary discourse surrounding the process of environmental reform in late-modern societies. This study examines dimensions of trust from stakeholders and uses a group process to enrich the data describing and explaining the reasons behind a possible ‘trust deficit ’ in the context of environmental governance and policy making in Hong Kong. Results from focus groups indicate that trust in government with regard to environmental issues is generally very low. Factors include poor leadership, a rather out-dated mindset of the government, inflexible government structures, inconsistent governance, misplaced knowledge and expertise in the government and its reluctance to create dialogue or communication. Stakeholders suggested that to enhance public trust in the government, the government needed to develop stronger leadership, reform government structure, improve communication on environmental issues to the public, take input from the community more seriously and make better use of regulation and provide incentives for environmental protection.
Auranofin, at clinically achievable dose, protects mice and prevents recurrence from Clostridioides difficile infection (2020)

Clostridioides difficile is the leading cause of nosocomial infections and a worldwide urgent public health threat. Without doubt, there is an urgent need for new effective anticlostridial agents due to the increasing incidence and severity of C. difficile infection (CDI). The aim of the present study is to investigate the in vivo efficacy of auranofin (rheumatoid arthritis FDA-approved drug) in a CDI mouse model and establish an adequate dosage for treatment. The effects of increased C. difficile inoculum, and pre-exposure to simulated gastric intestinal fluid (SGF) and simulated intestinal fluid (SIF), on the antibacterial activity of auranofin were investigated. Auranofin's in vitro antibacterial activity was stable in the presence of high bacterial inoculum size compared to vancomycin and fidaxomicin. Moreover, it maintained its anti-C. difficile activity after being exposed to SGF and SIF. Upon testing in a CDI mouse model, auranofin at low clinically achievable doses (0.125 mg/kg and 0.25 mg/kg) significantly protected mice against CDI with 100% and 80% survival, respectively. Most importantly, auranofin (0.125 mg/kg and 0.25 mg/kg) significantly prevented CDI recurrence when compared with vancomycin. Collectively, these results indicate that auranofin could potentially provide an effective, safe and quick supplement to the current approaches for treating CDI.
Validity and reliability of Work Productivity and Activity Impairment among patients with axial spondyloarthritis in Singapore (2020)

This study supports the content validity, construct validity, and test-retest reliability of the WPAI in patients with axSpA.
Supplementary Figure S11 from Dissecting the Mechanisms Underlying the Cytokine Release Syndrome (CRS) Mediated by T-Cell Bispecific Antibodies (2023)

&lt;p&gt;Supplementary Figure S11. Single cell RNA-sequencing of whole blood treated with 0.2 μg/mL CD20-TCB was performed using the BD Rhapsody platform. A. UMAP plots of neutrophils colored by treatment. B. UMAP plots showing IL-1β (IL1B), IL-1Ra (IL1RN), IL-6 (IL6), IP-10 (CXCL10), IL-8 (CXCL8), TNF-α (TNF), MCP-1 (CCL2), MIP-1α (CCL3), MIP-1β (CCL4) gene expression within neutrophil clusters identified above. C. Violin plots showing the kinetics of gene expression distribution in neutrophils. The cytokine and cytokine receptor genes are listed on the y axis. The color code on the right side of the violin plots represents the median gene expression. The untreated condition corresponds to the pooled baseline and untreated samples. N= 4 donors (baseline, and 20 hrs) and n=2 donors (2 hrs, 4 hrs and 6 hrs).&lt;/p&gt;
Resynchronization and Controllability of Bursty Service Requests (2004)

There is an increasing prevalence of interactive Web sessions in the Internet. These are mostly short-lived TCP connections that are delay-sensitive and have transfer times dominated by TCP backoffs, if any, during connection establishment. Unfortunately, arrivals of such connections at a server tend to be bursty, and can trigger multiple retransmissions, resulting in long average client-perceived delays. Traditional traffic control mechanisms, such as token bucket filters, are designed to complement admission control mechanisms, by regulating throughput, bounding service times, and protecting systems from overload. However, they cannot control connection-establishment delays, and thus, do not provide effective control of client-perceived delays. We first present the surprising discovery of a resynchronization property of retransmitted requests that exacerbates client-perceived delays when traditional control mechanisms are used. Then, we introduce a novel, multistage filtering scheme called Abacus Filters (AFs) that limits the client-perceived delay while maximizing server throughput even in the case of bursty connection arrivals. Analysis of delay-control properties of various filtering mechanisms is presented, along with a detailed performance evaluation. AFs are shown to provide tight delay control and better complement traditional admission control policies.
Hidden-Markov-Model Analysis Of Telemanipulator Data (1991)

Mathematical model and procedure based on hidden-Markov-model concept undergoing development for use in analysis and prediction of outputs of force and torque sensors of telerobotic manipulators. In model, overall task broken down into subgoals, and transition probabilities encode ease with which operator completes each subgoal. Process portion of model encodes task-sequence/subgoal structure, and probability-density functions for forces and torques associated with each state of manipulation encode sensor signals that one expects to observe at subgoal. Parameters of model constructed from engineering knowledge of task.
Synthesis and characterisation of a new benzamide-containing nitrobenzoxadiazole as a GSTP1-1 inhibitor endowed with high stability to metabolic hydrolysis (2019)

The antitumor agent 6-((7-nitrobenzo[<i>c</i>][1,2,5]oxadiazol-4-yl)thio)hexan-1-ol (<b>1</b>) is a potent inhibitor of GSTP1-1, a glutathione <i>S</i>-transferase capable of inhibiting apoptosis by binding to JNK1 and TRAF2. We recently demonstrated that, unlike its parent compound, the benzoyl ester of <b>1</b> (compound <b>3</b>) exhibits negligible reactivity towards GSH, and has a different mode of interaction with GSTP1-1. Unfortunately, <b>3</b> is susceptible to rapid metabolic hydrolysis. In an effort to improve the metabolic stability of <b>3</b>, its ester group has been replaced by an amide, leading to <i>N</i>-(6-((7-nitrobenzo[<i>c</i>][1,2,5]oxadiazol-4-yl)thio)hexyl)benzamide (<b>4</b>). Unlike <b>3</b>, compound <b>4</b> was stable to human liver microsomal carboxylesterases, but retained the ability to disrupt the interaction between GSTP1-1 and TRAF2 regardless of GSH levels. Moreover, <b>4</b> exhibited both a higher stability in the presence of GSH and a greater cytotoxicity towards cultured A375 melanoma cells, in comparison with <b>1</b> and its analog <b>2</b>. These findings suggest that <b>4</b> deserves further preclinical testing.
An asymmetric synthesis of novel aminocyclopropyl carboxylic acids (ACC) (1995)

M. Es-Sayed, P. Devine, L. E. Burgess, A. de Meijere and A. I. Meyers, J. Chem. Soc., Chem. Commun., 1995, 141 DOI: 10.1039/C39950000141
A practical method for long‐range forecasting of ice severity in the Beaufort Sea (2002)

A linear regression model is used to forecast end of summer ice conditions in the Beaufort Sea with a few months lead‐time. The model retains four sea ice and atmospheric parameters, where decreased spring total and winter multiyear ice concentrations, negative October East Atlantic phases, and positive March North Atlantic Oscillation phases are associated with lighter sea ice conditions. Monte Carlo simulations suggest that the results are not adversely affected by artificial skill, while Durbin‐Watson and Variance Inflation Factor statistics imply the final model is statistically valid. Cross validation diagnostics indicate that variations in the four predictors are related to 85% of the variation in sea ice conditions, suggesting that a relatively simple ice‐atmosphere statistical model can be used to forecast end of summer ice conditions in the Beaufort Sea.
MRN1 Implicates Chromatin Remodeling Complexes and Architectural Factors in mRNA Maturation (2012)

A functional relationship between chromatin structure and mRNA processing events has been suggested, however, so far only a few involved factors have been characterized. Here we show that rsc nhp6ΔΔ mutants, deficient for the function of the chromatin remodeling factor RSC and the chromatin architectural proteins Nhp6A/Nhp6B, accumulate intron-containing pre-mRNA at the restrictive temperature. In addition, we demonstrate that rsc8-ts16 nhp6ΔΔ cells contain low levels of U6 snRNA and U4/U6 di-snRNA that is further exacerbated after two hours growth at the restrictive temperature. This change in U6 snRNA and U4/U6 di-snRNA levels in rsc8-ts16 nhp6ΔΔ cells is indicative of splicing deficient conditions. We identify MRN1 (multi-copy suppressor of rsc nhp6ΔΔ) as a growth suppressor of rsc nhp6ΔΔ synthetic sickness. Mrn1 is an RNA binding protein that localizes both to the nucleus and cytoplasm. Genetic interactions are observed between 2 µm-MRN1 and the splicing deficient mutants snt309Δ, prp3, prp4, and prp22, and additional genetic analyses link MRN1, SNT309, NHP6A/B, SWI/SNF, and RSC supporting the notion of a role of chromatin structure in mRNA processing.