2312 IMPLANTATION OF RE-ENDOTHELIALIZED ACELLULAR KIDNEY SCAFFOLDS FOR WHOLE ORGAN ENGINEERING (2013)

You have accessJournal of UrologyTransplantation & Vascular Surgery: Renal Transplantation, Renal Vascular Surgery (II)1 Apr 20132312 IMPLANTATION OF RE-ENDOTHELIALIZED ACELLULAR KIDNEY SCAFFOLDS FOR WHOLE ORGAN ENGINEERING In Kap Ko, Mehran Abolbashari, Cheil Kim, Giuseppe Orlando, Mahmoudreza Moradi, Tamer Aboushwreb, John Jackson, Anthony Atala, and James Yoo In Kap KoIn Kap Ko Winston Salem, NC More articles by this author , Mehran AbolbashariMehran Abolbashari Winston Salem, NC More articles by this author , Cheil KimCheil Kim Winston Salem, NC More articles by this author , Giuseppe OrlandoGiuseppe Orlando Winston Salem, NC More articles by this author , Mahmoudreza MoradiMahmoudreza Moradi Winston Salem, NC More articles by this author , Tamer AboushwrebTamer Aboushwreb Winston Salem, NC More articles by this author , John JacksonJohn Jackson Winston Salem, NC More articles by this author , Anthony AtalaAnthony Atala Winston Salem, NC More articles by this author , and James YooJames Yoo Winston Salem, NC More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.2253AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The lack of transplantable allografts for renal failure has prompted the development of scaffold design and cell isolation techniques in order to re-create an engineered whole kidney. Decellularized solid organ xenografts such as the kidney hold great promise due to the maintenance of structural integrity while removing all cellular components. However, acelluar kidney vasculature needs an endothelial cell lining to avoid blood clotting and thrombus formation when transplanted into a living recipient. We previously have demonstrated that re-endothelialization of entire vasculature of decellularized kidney scaffolds is possible. In this study, we aimed to evaluate the effectiveness of re-endothelialization in vivo through implantation in a porcine model. METHODS Acellular porcine kidney scaffolds seeded with endothelial cells (MS1) expressing GFP was cultured in a bioreactor system. After perfusion bioreactor culture, the cell-seeded scaffold was implanted into pigs by arterial and venous anastomosis. Doppler ultrasound and angiography were performed to confirm patency of vessels after implantation. Histomorphological analyses were performed to determine the levels of re-endothelialization of vasculatures in the renal scaffold. RESULTS Uniform seeding of endothelial cells on the decellularized porcine kidneys was confirmed by H&E staining and SEM analysis. Organized endothelial cells were shown on the artery, vein, and smaller blood vessels without apparent clogging of cells at the capillary structures in the cortex. Furthermore, MS1 seeded scaffold displayed significant inhibition of platelet adhesion on cell-attached surfaces as compared to the strong platelets deposition found on the unseeded scaffolds. Patency of both renal artery and vein was confirmed by Doppler ultrasound after implantation. Angiography of the harvested kidneys showed intact vascular trees, indicating of patent vasculature network. Histological examination confirmed that most of the vasculatures in the kidney parenchyma were homogeneously coated with endothelial cells with minimal platelet adhesion. CONCLUSIONS These results show that implantation of re-endothelialized porcine kidney scaffolds prevents the formation of blood clots and maintains flow after implantation. This study demonstrates the possibility of recreating an engineered whole kidney for transplantation. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e948 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information In Kap Ko Winston Salem, NC More articles by this author Mehran Abolbashari Winston Salem, NC More articles by this author Cheil Kim Winston Salem, NC More articles by this author Giuseppe Orlando Winston Salem, NC More articles by this author Mahmoudreza Moradi Winston Salem, NC More articles by this author Tamer Aboushwreb Winston Salem, NC More articles by this author John Jackson Winston Salem, NC More articles by this author Anthony Atala Winston Salem, NC More articles by this author James Yoo Winston Salem, NC More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...