gebhart/patrepeval huggingface dataset:
Interferon-induced cell cycle changes in human hematopoietic cell lines and fresh leukemic cells. (1984)

A panel of 26 human hematopoietic cell lines was tested for sensitivity to growth inhibition towards interferon-alpha (IFN-alpha) by estimating the effects on cell cycle phase distribution using flow cytometry analysis. The proportion of proliferating cells was assessed by studying the fractional increase of cells in mitosis during a 24-hr vinblastine block. Of 26 cell lines tested, 17 were sensitive to IFN-alpha, and the main cell cycle effect was accumulation in the G0-G1 phase. One Burkitt's lymphoma line, Namalwa, showed a decreased rate of progress through S without any G0/G1 accumulation. Three of the cell lines were also tested with IFN-beta and with IFN-alpha 2 produced by recombinant DNA technology. The latter IFN did not affect one of the cell lines; otherwise, the results were similar to those of IFN-alpha. Six of 16 clinical specimens from patients with hematopoietic neoplasias were IFN-sensitive, all displaying a G0-G1 block. Our results indicate that IFN sensitivity is an individually linked property unrelated to cell origin.
Abstract 4489: Preclinical profile of novel, potent, and selective PI3K delta inhibitors (2011)

Abstract Background: Phosphoinositide-3 kinase (PI3K) belongs to a class of intracellular lipid kinases that phosphorylate the 3 position hydroxyl group of the inositol ring of phosphotidylinositol. The PI3K pathway is frequently activated in human cancers and thus represents an attractive target for small molecule inhibitors. Pan-PI3K inhibitors currently in development have been associated with adverse side-effects such as insulin resistance, thus necessitating the need to develop isoform specific inhibitors of PI3K. Herein, we describe the biological and pharmacokinetic properties of representative molecules from a series of novel and small molecule PI3KΔ inhibitors with scope to be further developed as clinical candidates for hematological malignancies such as acute myeloid leukemia and Non-Hodkin's lymphoma. Methods: Activity of test compounds on individual PI3K isoforms was determined by a Homogenous Time Resolved Fluorescence assay (Millipore, Billerica, MA) with modifications. Cell based selectivity assays against α, β, or γ isoforms was assessed by testing the effect of the compounds on PDGF, LPA, or c5a induced Akt phosphorylation in NIH-3T3 or RAW cells. Similarly, inhibition of cellular PI3KΔ activity was determined in a IgM induced human B-cell proliferation assay. Cell viability assay was conducted to determine the growth inhibitory effect of the compounds in cell lines representative of human acute and chronic leukaemia and lymphoma. Pharmacokinetic behaviour of compounds in plasma after single dose oral administration was determined in female Balb/c mice. Results: Among the compounds evaluated, RP5175 and RP5182 inhibited PI3KΔ activity in enzyme and cell based assays with IC50 values of 9.6 &amp; 37.3 nM and EC50 values of 7.6 &amp; 18.9 nM respectively. Besides, the compounds displayed a high degree of selectivity over the alpha (&amp;gt;500 fold), beta (&amp;gt;400 fold), and gamma (&amp;gt;15-40 fold) isoforms. Viability assays demonstrated that the compounds caused a significant inhibition in growth of THP-1, TOLEDO, and HL-60 cells. Pharmacokinetic studies in female Balb/c mice indicated good oral absorption with favourable peak plasma concentrations. Conclusions: Results demonstrate the PI3K delta selective nature of RP5175 and 5182 along with an ability to suppress proliferation of cancer cells. In vitro selectivity and potency data indicate the therapeutics potential of the compounds in hematological cancers without the deleterious effects commonly associated with the Pan PI3K inhibitors. The compounds are currently being tested for in vitro and in vivo efficacy across various cancer cell lines and xenograft models besides selectivity against other kinases. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 4489. doi:10.1158/1538-7445.AM2011-4489
Predicting changes in blood flow in patient-specific operative plans for treating aortoiliac occlusive disease (2005)

Traditionally, a surgeon will select a procedure for a particular patient on the basis of past experience with patients with a similar state of disease.The experience gained from this patient will be selectively used when treating the next patient with similar symptoms.This article describes a surgical planning system that was developed to enable a vascular surgeon to create and test alternative operative plans prior to surgery for a given patient.One-dimensional and three-dimensional hemodynamic (i.e., blood flow) simulations were performed for rest and exercise for operative plans for two aorto-femoral bypass patients and compared with actual postoperative data.The information obtained from one-dimensional (volume flow distribution and pressure losses) and three-dimensional (flow, pressure, and wall shear stress) hemodynamic simulations may be clinically relevant to vascular surgeons planning interventions.
Supplemental Information from BET Bromodomain Inhibitors Enhance Efficacy and Disrupt Resistance to AR Antagonists in the Treatment of Prostate Cancer (2023)

&lt;p&gt;Supplemental Materials and Methods&lt;/p&gt;
A RESPONSABILIDADE SOCIAL NO PROJETO "ESTAÇÃO DO LIVRO": leitura na praça (2012)

In the current social inclusion / exclusion has been a topic discussed at events in various areas of knowledge, making us reflect on how businesses and public authorities are acting on behalf of society. In this effort, are sometimes used projects that encourage the integration of those in the information society and knowledge. This reflection is the basis of research that aims to analyze the aspects of social responsibility developed in the municipal project Station of the Book that encourages the practice of reading as a social inclusion policy of the Municipality of Joao Pessoa / PB. Methodologically the research is characterized with quantiqualitative approach, and results of questionnaires to professionals of the three Station of the Book that at the time, which are established and in working with social responsibility. As subjects have been six professionals / trainees of the courses in Arts and Education and coordinator of the Library of working in stations. The data collected and analyzed suggest that professionals realize that the actions developed in the project are composed of social attitudes responsibly, linking actions to mediate the process of reading practice and social inclusion of the community. It is the social relevance of the Station of the Book because it represents an innovative experiment in the city of Joao Pessoa / PB. We highlight its role in developing planned actions of reading practices and social responsibility culminating in a continuous process of inclusion and citizenship. Emphasizes the need to improve physical, human, material and financial resources to carry out their actions and the fulfillment of their goals, assisting the existing stations and those that are to be implemented.

Keywords: social responsibility. Social inclusion. Practice reading. Information Professionals.
Aminoglycosides were associated with higher rates of surgical patent ductus arteriosus closure in preterm infants (2020)

First-line antibiotic treatment of preterm infants with aminoglycosides was associated with higher rates of surgical PDA closure.
Macrocycle‐Based Hydroxamate Ligands for Complexation and Immunoconjugation of 89Zirconium for Positron Emission Tomography (PET) Imaging (2016)

Four novel chelators (<b>L1</b>-<b>L4</b>) and their <sup>89</sup>zirconium complexes were prepared and compared with the <sup>89</sup>zirconium desferrioxamine B (DFO) complex. The new chelates are based on 1,4,7,10-tetraazacyclododecane (cyclen) and 1,4,8,11-tetraazacyclotetradecane (cyclam) scaffolds and present either three or four hydroxamate arms for coordination with Zr<sup>4+</sup> ions with coordination numbers between six and eight. The 89Zr-<b>L4</b> complex showed similar stability to that of <sup>89</sup>Zr-DFO when incubated in either rat blood plasma or ethylenediaminetetraacetic acid challenge experiments. Positron imaging and biodistribution studies in mice showed that <sup>89</sup>Zr-<b>L4</b> had similar pharmacokinetic behavior to that of <sup>89</sup>Zr-DFO, with rapid renal elimination and low residual activity in background tissues. A bifunctional version of <b>L4</b> (<b>L5</b>) was synthesized and conjugated to trastuzumab; an anti-HER2/neu antibody. Immunopositron emission tomography imaging and biodistribution with <sup>89</sup>Zr-<b>L5</b>-trastuzumab revealed high tumor to background ratios (tumor/blood ratio: 14.2 ± 2.25) and a high tumor specificity that was comparable to the performance of <sup>89</sup>Zr-DFO-trastuzumab.
Intracellular Antimicrobial Photodynamic Therapy: A Novel Technique for Efficient Eradication of Pathogenic Bacteria (2010)

The increasing resistance of bacteria to antibiotics is a serious problem, caused in part by excessive and improper use of these drugs. One alternative to traditional antibiotic therapy is photodynamic antimicrobial chemotherapy (PACT) which is based on the use of a photosensitizer (PS), activated by illumination with visible light. The poor penetration of visible light through the skin limits the application of PACT to the treatment of skin infections or the use of invasive procedures. To overcome this problem we report the exploitation of light emitted as a result of the chemiluminescent reaction of luminol to excite the PS and we call this process chemiluminescent photodynamic antimicrobial therapy (CPAT). We studied the effect of free and liposome-encapsulated PS (methylene blue or toluidine blue) on bacteria under excitation by either white external light or chemiluminescence emitted by free or liposome-enclosed luminol. PACT showed slightly better performance that CPAT for free and encapsulated PS for both types of bacteria. CPAT resulted in a three log suppression of Staphylococcus aureus and two log suppression of Escherichia coli growth. The use of CPAT may prove to be a novel and more effective form of antimicrobial therapy, particularly for internal infections not easily accessible to traditional PACT.
Effect of static mixer on fiber orientation of injection molded fiber‐reinforced composites (2022)

Abstract The effect of an in‐mold static mixer on orientation of fiber‐reinforced polypropylene (PP) was explored within the injection molding process. Several mold geometries and helical mixer designs were assessed via simulation to identify the mixing ability and the potential effect on fiber orientation. It was found that the static mixers within the runner segment could successfully mix the polymer and randomize the fibers but that the fiber alignment reduction was quickly recovered. Injection molding experiments were carried out to verify these simulations using one geometry case. Fiber orientation at different mold locations were measured using micro‐CT (μCT) scans and the degree of fiber orientation was quantified by “goodness of fit” to a normal Gaussian function approach. The experimental fiber orientation results showed good agreement with the simulations. These experiments indicated that the use of a static mixer within the runner system of a mold could be used for mixing the polymer melt after the plasticizing unit of the injection molding machine. However, its effect on changing the overall alignment of the fibers within injection molded parts could be offset by the melt flow downstream of the static mixer, suggesting the importance of mixer location with respect to the part cavity.
Identifiability of sparse structural equation models for directed and cyclic networks (2013)

Structural equation models (SEMs) provide a statistical description of directed networks. The networks modeled by SEMs may have signed edge weights, a property that is pertinent to represent the activating and inhibitory interactions characteristic of biological systems, as well as the collaborative and antagonist behaviors found in social networks, among other applications. They may also have cyclic paths, accommodating the presence of protein stabilizing loops, or the feedback in decision making processes. Starting from the mathematical description of a linear SEM, this paper aims to identify the topology, edge directions, and edge weights of the underlying network. It is established that perturbation data is essential for this purpose, otherwise directional ambiguities cannot be resolved. It is also proved that the required amount of data is significantly reduced when the network topology is assumed to be sparse; that is, when the number of incoming edges per node is much smaller than the network size. Identifying a dynamic network with step changes across time is also considered, but it is left as an open problem to be addressed in an extended version of this paper.
ChemInform Abstract: De Novo Designed Polypeptide Catalysts with Adopted Folded Structures (1999)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Rigidly foldable origami gadgets and tessellations (2015)

Rigidly foldable origami allows for motion where all deflection occurs at the crease lines and facilitates the application of origami in materials other than paper. In this paper, we use a recently discovered method for determining rigid foldability to identify existing flat-foldable rigidly foldable tessellations, which are also categorized. We introduce rigidly foldable origami gadgets which may be used to modify existing tessellations or to create new tessellations. Several modified and new rigidly foldable tessellations are presented.
Modelling mercury sorption of a polysulfide coating made from sulfur and limonene (2022)

A polymer made from sulfur and limonene was used to coat silica gel and then evaluated as a mercury sorbent. A kinetic model of mercury uptake was established for a range of pH values and concentrations of sodium chloride. Mercury uptake was generally rapid from pH = 3 to pH = 11. At neutral pH, the sorbent (500 mg with a 10 : 1 ratio of silica to polymer) could remove 90% of mercury within one minute from a 100 mL solution containing 5 ppm HgCl<sub>2</sub> and 99% over 5 minutes. It was found that sodium chloride, at concentrations comparable to seawater, dramatically reduced mercury uptake rates and capacity. It was also found that the spent sorbent was stable in acidic and neutral media, but degraded at pH 11 which led to mercury leaching. These results help define the conditions under which the sorbent could be used, which is an important advance for using this material in remediation processes.
HK-2 Human Renal Proximal Tubule Cells as a Model for G Protein–Coupled Receptor Kinase Type 4–Mediated Dopamine 1 Receptor Uncoupling (2010)

HK-2 human renal proximal tubule cells (RPTC) are commonly used in the in vitro study of "normal" RPTCs. We discovered recently that HK-2 cells are uncoupled from dopamine 1 receptor (D(1)R) adenylyl cyclase (AC) stimulation. We hypothesized that G protein-coupled receptor kinase type 4 (GRK4) single nucleotide polymorphisms may be responsible for the D(1)R/AC uncoupling in HK-2. This hypothesis was tested by genotyping GRK4 single nucleotide polymorphisms, measuring D(1)-like receptor agonist (fenoldopam)-stimulated cAMP accumulation, quantifying D(1)R inhibition of sodium transport, and testing the ability of GRK4 small interfering RNA to reverse the D(1)R/AC uncoupling. We compared HK-2 with 2 normally coupled human RPTC cell lines and 2 uncoupled RPTC cell lines. The HK-2 cell line was found to have 4 of 6 potential GRK4 single nucleotide polymorphisms known to uncouple the D(1)R from AC (namely, R65L, A142V, and A486V). AC response to fenoldopam stimulation was increased in the 2 normally coupled human RPTC cell lines (FEN: 2.02+/-0.05-fold and 2.33+/-0.19-fold over control; P<0.001; n=4) but not in the 2 uncoupled or HK-2 cell lines. GRK4 small interfering RNA rescued the fenoldopam-mediated AC stimulation in the uncoupled cells, including HK-2. The expected fenoldopam-mediated inhibition of sodium hydrogen exchanger type 3 was absent in HK-2 (n=6) and uncoupled RPTC cell lines (n=6) but was observed in the 2 normally coupled human RPTC cell lines (-25.41+/-4.7% and -27.36+/-2.70%; P<0.001; n=6), which express wild-type GRK4. Despite the fact that HK-2 cells retain many functional characteristics of RPTCs, they are not normal from the perspective of dopaminergic function.
ChemInform Abstract: Catalytic Asymmetric Vinylation of Ketone Enolates. (2001)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
<title>Measuring micro floor vibrations with distributed fiber optic sensors</title> (2001)

In certain applications, such as microelectronic manufacturing, monitoring and controlling small floor vibrations can be essential. This paper describes how an array of distributed fiber optic sensors can be used to monitor micro floor vibrations as part of an adaptive control strategy. The performance target is to limit vibrations in the 10 Hz frequency range to less than 10 microinches peak to peak. The distributed fiber optic sensors are of an interferometric design that uses a single mode to multi mode to single mode configuration. The fibers can be arranged in a grid so as to localize vibration hot spots. Data comparing the performance of the vibration sensing array with that of conventional point piezoelectric accelerometers, as well as sensing and control strategies will be presented.
Ultrathin monochromatic photonic power converters with nanostructured back mirror for light trapping of 1310-nm laser illumination (2021)

Photonic power converters (PPCs) are one of the main components of optical power transmission systems, generating electrical power via the photovoltaic effect. We simulate ultrathin PPCs designed for operating at the telecommunication wavelength of 1310 nm with 9 and 12 times thinner absorbing layers using cubic and pyramidal nanostructured back reflectors (BRs), respectively. While increasing efficiency by 13% (rel.) over conventional PPCs, results also show superior light trapping for pyramidal BR with twice the absorption of a simple double pass absorber layer of the same thickness and higher short-circuit current for pillar BR reaching 94% of an ideal Lambertian surface.
Trivalent NDV-HXP-S vaccine protects against phylogenetically distant SARS-CoV-2 variants of concern in mice (2022)

Equitable access to vaccines is necessary to limit the global impact of the coronavirus disease 2019 (COVID-19) pandemic and the emergence of new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants. In previous studies, we described the development of a low-cost vaccine based on a Newcastle Disease virus (NDV) expressing the prefusion stabilized spike protein from SARS-CoV-2, named NDV-HXP-S. Here, we present the development of next-generation NDV-HXP-S variant vaccines, which express the stabilized spike protein of the Beta, Gamma and Delta variants of concerns (VOC). Combinations of variant vaccines in bivalent, trivalent and tetravalent formulations were tested for immunogenicity and protection in mice. We show that the trivalent preparation, composed of the ancestral Wuhan, Beta and Delta vaccines, substantially increases the levels of protection and of cross-neutralizing antibodies against mismatched, phylogenetically distant variants, including the currently circulating Omicron variant.
Morphotopography of the Roots of Jugular Vein in Sheep (2010)

The study material was represented by 10 specimens of sheep. Mainly, it was
described the maxillary and superficial temporal veins with major tributaries of each. Invariably, it
was noted the presence of buccal and profunda faciei veins, matter which was not reported in the
literature.
Electrochemically Sensitized Luminescence from Lanthanides in d‐/f‐Block Heteronuclear Arrays (2017)

Abstract A new class of electrochemiluminescent (ECL) system is reported in which a heterobimetallic array is used to facilitate electrochemically initiated lanthanide emission, following energy transfer from an ECL‐active donor. Spectroscopic measurements are used to probe the efficiency, rate and mechanism of the energy‐transfer process in two bimetallic systems with differing donor energies. Non‐optical sensitization of the luminescence from lanthanide ions opens up exciting new possibilities for the development of ECL probes with sharper emission peaks and a greater variety of emission colours.
Oral rinse MMP‐8 point‐of‐care immuno test identifies patients with strong periodontal inflammatory burden (2010)

Oral rinse MMP-8 together with TIMP-1 analysis may have potential in complementary periodontal diagnostics. dentoELISA can be applied in quantitative oral rinse chair side biomarker diagnostics.
Dual Pressure from Antiretroviral Therapy and Cell-Mediated Immune Response on the Human Immunodeficiency Virus Type 1 Protease Gene (2003)

Human immunodeficiency virus (HIV)-specific CD8(+) T-lymphocyte pressure can lead to the development of viral escape mutants, with consequent loss of immune control. Antiretroviral drugs also exert selection pressures on HIV, leading to the emergence of drug resistance mutations and increased levels of viral replication. We have determined a minimal epitope of HIV protease, amino acids 76 to 84, towards which a CD8(+) T-lymphocyte response is directed. This epitope, which is HLA-A2 restricted, includes two amino acids that commonly mutate (V82A and I84V) in the face of protease inhibitor therapy. Among 29 HIV-infected patients who were treated with protease inhibitors and who had developed resistance to these drugs, we show that the wild-type PR82V(76-84) epitope is commonly recognized by cytotoxic T lymphocytes (CTL) in HLA-A2-positive patients and that the CTL directed to this epitope are of high avidity. In contrast, the mutant PR82A(76-84) epitope is generally not recognized by wild-type-specific CTL, or when recognized it is of low to moderate avidity, suggesting that the protease inhibitor-selected V82A mutation acts both as a CTL and protease inhibitor escape mutant. Paradoxically, the absence of a mutation at position 82 was associated with the presence of a high-avidity CD8(+) T-cell response to the wild-type virus sequence. Our results indicate that both HIV type 1-specific CD8(+) T cells and antiretroviral drugs provide complex pressures on the same amino acid sequence of the HIV protease gene and, thus, can influence viral sequence evolution.