gebhart/patrepeval huggingface dataset:
Further evidence for a common mechanism for shedding of cell surface proteins (1997)

Pro-TNF alpha, Steel factor, type II IL-1R and IL-2R alpha were expressed in COS-7 cells and the generation of their soluble forms was examined. The release of all four proteins was strongly stimulated by the phorbol ester PMA and completely blocked by a hydroxamate-based inhibitor of metalloproteases. COS-7 cell membranes were found to cleave various synthetic pro-TNF alpha peptides with the same specificity as a partially purified TNF alpha converting enzyme purified from human monocytic cells, suggesting that the same enzyme may be responsible for at least some of the COS-7 cell shedding activity.
EVEREST: Everolimus for renal cancer ensuing surgical therapy—A phase III study (SWOG S0931, NCT01120249). (2022)

LBA4500 Background: Patients (pts) who undergo resection of renal cell carcinoma (RCC) with curative intent remain at risk for disease relapse. We conducted a phase III, double-blind, placebo (PB)-controlled, intergroup study to determine the effect of adjuvant treatment with the mTOR inhibitor everolimus (EVE) on recurrence-free survival (RFS). Methods: Pts with treatment-naïve, non-metastatic, fully-resected RCC at intermediate high- (pT1 G3-4 N0 to pT3a G1-2 N0) or very high-risk (pT3a G3-4 to pT4 G-any or N+) for recurrence were randomized 1:1 to EVE 10 mg PO daily x 54 weeks or PB within 12 weeks of radical or partial nephrectomy. Randomization was stratified by risk group, histology (clear vs. non-clear cell), and performance status (0 vs. 1). RFS was the primary end point; secondary endpoints included overall survival (OS) and adverse events (AEs). The study was designed to detect an 18% reduction in the risk of RFS with EVE compared to PB, corresponding to an improvement of median RFS from 6.75 (based on E2805 ASSURE) to 8.23 years. Final analysis, using a stratified logrank test, was to occur after 804 total events or by 3/2022, whichever occurred first. Results: Between 4/2011 and 9/2016, 1545 pts were randomized to EVE (n = 775) or PB (n = 770). Overall pt characteristics included: intermediate high-/very high-risk 45%/55%; clear cell/non-clear cell 83%/17%. The DSMC recommended study continuation after each of 4 pre-specified interim analyses. 556 DFS events among 1499 eligible pts occurred by the time of final study analysis on 2/23/2022. The median follow-up was 76 months. RFS was improved with EVE vs. PB (HR 0.85, 95% CI, 0.72 – 1.00; P 1-sided = 0.0246), narrowly missing the pre-specified, one-sided significance level of 0.022 which accounted for interim analyses. Median RFS was not reached; the 6-year RFS estimate was 64% for EVE and 61% for PB. RFS improvement with EVE vs. PB was observed in the very high-risk group (HR 0.79, 95% 0.65-0.97; P 1-sided = 0.011) but not in the intermediate high-risk group (HR 0.99, 95% CI 0.73-1.35, P 1-sided = 0.48) ( P for interaction = 0.22). With 290 deaths, OS was similar between arms (HR 0.90, 95% CI, 0.71 – 1.13; P 1-sided = 0.178). Fewer pts completed all 54 weeks of study treatment in the EVE group (45% v 69%). In the EVE group, 37% withdrew due to AEs (vs 5% in PB). Grade 3-4 AEs occurred in 46% of pts treated with EVE and 11% with PB. The most common grade 3-4 AEs were mucositis (14% v 0%), hypertriglyceridemia (11% vs. 2%), and hyperglycemia (5% vs. 0%). Conclusions: Adjuvant EVE improved RFS in RCC pts after nephrectomy, but the nominal significance level was narrowly missed. The RFS improvement was seen despite a high rate of early treatment discontinuation. A 21% improvement in RFS with EVE was observed in pts with very high-risk disease, a group for whom adjuvant therapy may be most relevant. Clinical trial information: NCT01120249.
A Small-RNA Enhancer of Viral Polymerase Activity (2012)

Influenza A virus (IAV) is an unremitting virus that results in significant morbidity and mortality worldwide. Key to the viral life cycle is the RNA-dependent RNA polymerase (RdRp), a heterotrimeric complex responsible for both transcription and replication of the segmented genome. Here, we demonstrate that the viral polymerase utilizes a small RNA enhancer to regulate enzymatic activity and maintain stoichiometric balance of the viral genome. We demonstrate that IAV synthesizes small viral RNAs (svRNAs) that interact with the viral RdRp in order to promote genome replication in a segment-specific manner. svRNAs localize to the nucleus, the site of IAV replication, are synthesized from the positive-sense genomic intermediate, and interact within a novel RNA binding channel of the polymerase PA subunit. Synthetic svRNAs promote polymerase activity in vitro, while loss of svRNA inhibits viral RNA synthesis in a segment-specific manner. Taking these observations together, we mechanistically define svRNA as a small regulatory enhancer RNA, which functions to promote genome replication and maintain segment balance through allosteric modulation of polymerase activity.
Parotid Fat Contents in Healthy Subjects Evaluated with Iterative Decomposition with Echo Asymmetry and Least Squares Fat-Water Separation (2013)

The IDEAL method provided a high success rate for parotid fat measurements, even in subjects with metallic dental implants.
Transmission electron microscopy enables the reconstruction of the catenane and ring forms of CS2 hydrolase (2014)

Transmission electron microscopic studies on CS2 hydrolase provide direct evidence for the existence of the hexadecameric catenane and octameric ring topologies. Reconstructions of both protein assemblies are in good agreement with crystallographic analyses of the catenane and ring forms of CS2 hydrolase.
User Interaction Techniques for Future Lighting Systems (2011)

LED-based lighting systems have introduced radically new possibilities in the area of artificial lighting. Being physically small the LED can be positioned or embedded into luminaires, materials and even the very fabric of a building or environment. Together with new functionality and flexibility comes complexity; the simple light switch is not anymore sufficient to control our light. This workshop explores new ways of interacting with light. The goal is to define directions for new forms of user interaction that will be able to support the emerging LED-based lighting systems.
A concise route to MK-4482 (EIDD-2801) from cytidine (2020)

A two-step route to MK-4482 (EIDD-2801, 1) was developed consisting of an esterification and hydroxamination of cytidine.
P630 Recovery of non-bacterial thrombotic endocarditis and severe aortic regurgitation in a young patient with acute promyelocytic leukemia (2020)

Abstract Introduction Non-bacterial thrombotic endocarditis (NBTE) is characterised by non-infectious vegetations on the cardiac valves and is typically seen in patients with malignancies or systemic lupus erythematosus. Risk of thromboembolic events is high and treatment of choice consists of anticoagulation, if feasible, as well as treatment of the underlying disease. Severe valvular dysfunction is rarely seen and in these cases, surgical valve replacement is usually performed. Case presentation A 28-year old female patient presented with left-sided hemiparesis persisting for several hours. Angio-CT scan of the brain revealed a branch occlusion of the middle cerebral artery with a correlating infarct core and without an area of penumbra. Blood tests showed decreased thrombocyte levels (45 x109/l). Given the thrombocytopenia and the time between the onset of symptoms and presentation, no thrombolysis was administered. A subsequently performed transoesophageal echocardiography for diagnostic work-up of cardioembolic sources of stroke revealed a right ventricular thrombus and vegetations on the aortic valve causing severe aortic regurgitation with a holodiastolic flow reversal in the descending aorta (Fig. 1 (a) and (b) showing a large central coaptation defect). There were no of signs of infection or inflammation and blood cultures remained negative, thus infectious endocarditis was unlikely. A bone marrow biopsy led to the diagnosis of acute promyeolocytic leukemia (PML). Despite the inherent high risk of bleeding due to PML, anticoagulation with unfractionated heparin was initiated and treatment with prednisolone, all-trans retinoic acid and arsenic trioxide was started. After three weeks of therapy aortic valve vegetation size decreased considerably leading to dramatic reduction of aortic regurgitation (Fig. 1 (c) and (d)). No further embolic event or bleeding occurred. Conclusion This case is remarkable in many aspects. First, PML typically causes bleeding complications whereas NBTE is characterised by a high risk of thromboembolic events. Thus, the decision to start or not to start anticoagulation is challenging. Second, NBTE, unlike infectious endocarditis, rarely causes severe valvular dysfunction and reported treatment in most cases consisted of surgical valve replacement or repair. However, in this case, conservative treatment alone led to almost complete resolution of valvular vegetations and of aortic regurgitation. We conclude that conservative treatment of severe valvular disease in the setting of NBTE should be strongly considered, especially given the small number of cases reported worldwide. Abstract P630 Figure. Fig.1
Cyclic AMP does not mediate inhibition of DNA synthesis by interferon in mouse Swiss 3T3 cells (1984)

The purpose of this study was to determine whether cyclic AMP (cAMP) plays any direct or indirect role in the antiproliferative effect of mouse L-cell interferon in Swiss 3T3 cells. Firstly, we found that interferon did not affect intracellular levels of cAMP in these cells in the absence or the presence of cAMP-elevating agents. Secondly, we examined the effect of interferon on the stimulation of DNA synthesis of quiescent 3T3 cells by a range of cyclic AMP-elevating agents, including cholera toxin, cAMP derivatives, and prostaglandin E, added in the presence of insulin or vasopressin. Interferon inhibited cyclic AMP-stimulated DNA synthesis as measured by incorporation of radioactive thymidine into acid-insoluble material and autoradiographic analysis of the fraction of labelled cells. Dose-response curves and kinetics of inhibition were identical to those obtained in cultures stimulated by combinations of growth factors that do not increase the intracellular level of cAMP. The inhibition by interferon of cAMP-stimulated DNA synthesis was also observed in secondary cultures of mouse embryo fibroblasts, where cAMP-elevating agents provide a mitogenic signal in the absence of other added growth factors. These results show that the inhibitory effect of interferon on DNA synthesis in Swiss 3T3 cells is not mediated by cyclic AMP.
Electrodeposition of Gold Particles on Aluminum Substrates Containing Copper (2006)

Abstract not Available.
Fiber Optic Pulsed Laser Delivery For Remote Measurements (1987)

The results of a research program on the delivery of high-peak-power laser light via fiber optics are presented. We discuss the influence of the host medium and the optical signals on the choice of fiber materials, complemented by a consideration of the measurement environment's effects on the quality of the data. We pay close attention to the choice of input/output beam/fiber coupling optics, nonlinear processes in the core, measurement system noise, and baseline drifts. Useful discussions of pulsed laser damage to optical fibers and data for optimization of a given fiber optic laser beam delivery system are given. As an example, details are given of the optical and instrumentational aspects of a particular fiber optic system developed for remote sensing of pressures and temperatures of UF6 gas in an operating advanced gas centrifuge.
Expression, purification, and characterization of human factor XIII in Saccharomyces cerevisiae (1990)

Factor XIII is the terminal enzyme of the clotting cascade. A cDNA sequence encoding human placental factor XIII was expressed in Saccharomyces cerevisiae with the yeast ADH2-4c promoter. Expression levels were a strong function of the noncoding flanking DNA content of the construction. When the terminal 3'-flanking noncoding DNA was removed, expression increased approximately 50-fold. The protein was produced in quantity by high-yield fermentation and purified to homogeneity. The recombinant protein was cleaved by thrombin at the same activation site as purified human placental FXIII and exhibited 100% enzymatic activity. At high thrombin concentrations rFXIIIa was cleaved into inactive 54- and 25-kDa polypeptides. The identity of these cleavage sites and the blocked N-terminus to that of the human protein was revealed by amino acid microsequencing. A time course of thrombin activation was performed and the relative distribution of the thrombin-cleaved subunits to the uncleaved zymogen subunits determined; the results were consistent with the half of the sites catalytic model for transglutaminase activity proposed by Chung et al. (Chung, S. I., Lewis, M. S., & Folk, J. E. (1974) J. Biol. Chem. 249, 940-950, 1974) and Hornyak et al. (Hornyak, T. J., Bishop, P. D., & Shafer, J. A. (1989) Biochemistry 28, 7326-7332). Equilibrium and velocity sedimentation analysis indicated that rFXIII exists as a 166-kDa nondissociating dimer that behaves as a compact particle of 8.02 S. Thus, all of the properties of rFXIII thus far examined are consistent with those reported for human platelet and placental FXIII.(ABSTRACT TRUNCATED AT 250 WORDS)
Cost based hierarchical control for the management of a DC microgrid (2017)

Microgrids have become an alternative to increase the controlability and resilience of the electrical system due to the local and efficient energy management they provide. Additionally, when the microgrid has a main DC bus it does not require synchronization hence the control scheme is simplified allowing to improve the dynamics of currents and voltages. These DC microgrids are usually controlled using a three-level hierarchical scheme associated to different elements in the microgrid in which the third control level optimizes the operation of the whole microgrid in term of costs and efficiency. In this paper a tertiary control level is proposed for a DC microgrid which optimizes its operation in terms of costs but at the same time takes into account restrictions of the grid connection and battery state of charge. Simulation results with realistic operating conditions show a good performance in terms of dynamic response and steady state operation while an optimize economic operation is achieved.
Odontogenic Differentiation of Human Dental Pulp Stem Cells on Hydrogel Scaffolds Derived from Decellularized Bone Extracellular Matrix and Collagen Type I (2016)

These results demonstrate the potential of the bECM hydrogel scaffolds to stimulate odontogenic differentiation of DPSCs.
ChemInform Abstract: The Quest for Extra‐Large Pore, Crystalline Molecular Sieves (1998)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
New results on adaptive computational resource allocation in soft MIMO detection (2011)

The fundamental problem of our interest is soft MIMO detection for the case of block fading, i.e., when the transmitted codeword spans over several independent channel realizations. We develop methods that adaptively allocate computational resources to the detection problems of each channel realization, under a total per-codeword complexity constraint. The new results consist of a new algorithm, a new performance measure, and a thorough complexity discussion.
Antisense Oligonucleotide-Mediated Correction of Transcriptional Dysregulation is Correlated with Behavioral Benefits in the YAC128 Mouse Model of Huntington's Disease (2013)

The role of transcriptional dysregulation in the pathogenesis of Huntington's disease is not well understood, however, a wealth of evidence now strongly suggests that changes in transcriptional signatures are a prominent feature in the brains of both HD patients and animal models of the disease. Our study is the first to show that a therapeutic agent capable of improving an HD disease phenotype is concomitantly correlated with normalization of a subset of dysregulated striatal transcripts. Our data suggests that correction of these disease-altered transcripts may underlie, at least in part, the therapeutic efficacy shown associated with ASO-mediated correction of HD phenotypes and may provide a novel set of early biomarkers for evaluating future therapeutic concepts for HD.
From Huelsmeyer's telemobiloskop to the digital radar (2014)

The year 2014 is for all radar engineers and for the international radar society of much importance due to the first radar patent which has been applied 110 years ago on April 30th, 1904 in Berlin at the “Kaiserliche Patentamt”, the former German patent office. The inventor, Christian Huelsmeyer, was interested in collision avoidance systems for ship traffic which was an important economical factor at that time. The TITANIC disaster could have been avoided if the Telemobiliscop technique had been applied to this ship. Starting the development with analogue technique and continuing with digital and semiconductor technology changed the radar system design and the resulting performance totally. The challenge today is to design radars on a single chip.
The quaternion-based anisotropic gradient for the color images (2019)

Image gradient, as a preprocessing step is an essential tool in image processing in many research areas such as edge detection, segmentation, smoothing, inpainting, etc. In the present paper, we develop a new gradient by integrated the quaternion framework with LPA-ICI (local polynomial approximation &#x2013; the intersection of confidence intervals) based on anisotropic gradient concepts for the color image processing applications. Computer simulations on the Berkeley Segmentation Dataset show that the new quaternion anisotropic gradient exhibits fewer color artifacts compared to state-of-the-art techniques including well-knowledge gradient operators.
Comparison of 3-D reconstruction with 3D-OSEM and with FORE+OSEM for PET (2001)

The combination of Fourier rebinning (FORE) and the ordered subsets expectation-maximization (OSEM), a fast statistical algorithm, appears as a promising alternative to the fully three-dimensional (3-D) iterative approach for clinical positron emission tomography (PET) data. In this paper, we evaluated the properties of FORE+OSEM and compared it with fully 3-D OSEM using both simulations and data acquired by commercial scanners. The aim is to determine to what extent the speed advantage of FORE+OSEM is paid for by a possible degradation of image quality in the case of noisy clinical PET data. A forward- and back-projection pair based on a line integral model was used in two-dimensional OSEM and 3-D OSEM (3D-OSEM) instead of a system matrix. Different variants of both approaches have been studied with simulations in terms of contrast-noise tradeoff. Two variants--FORE+OSEM with attenuation weighting (AW) [FORE+OSEM(AW)] and 3D-OSEM with attenuation-normalization weighting (ANSP) and a shifted-Poisson (SP) model [3D-OSEM(ANSP)]--were compared with measured phantom data and patient data. Based on the results from both simulations and measured data, we conclude that: 1) both attenuation (-normalization) weighting and the SP model improve the image quality but slow down the convergence and 2) despite its approximate nature, FORE+OSEM does not show apparent image degradation compared with 3D-OSEM for data with a noise level typical of a whole-body FDG scan.
A guided tour to virtual Sagalassos (2001)

The system presented in this paper allows to take a guided tour to a virtual reconstruction of the ancient site of Sagalassos. The site has been reconstructed by combining 3D models of the landscape and remains with CAD reconstructions of monuments based on archaeological hypothesis. The 3D models were obtained using advanced image-based modelling techniques developed over the last few years in computer vision. The visitor can communicate with the virtual guide through natural speech. The guide itself is represented by a 3D face model.
Daytime Sleepiness is Associated with Falls in Parkinson's Disease (2013)

Falls are frequent in Parkinson's disease (PD), and may be influenced by daytime sleepiness. We reviewed the records of 120 men with PD. Mean Epworth Sleepiness Scale (ESS) values were significantly different between non-fallers and fallers (6.0 vs. 9.7, p < 0.01). In multivariate analysis, ESS remained significantly associated with falls (OR 1.2, 95% CI 1.1-1.4, p = 0.02), along with cognitive impairment (OR 4.4 95% CI 1.0-18.7, p = 0.04) and postural instability/gait dysfunction (OR 1.6 95% CI 1.0-2.4, p = 0.03) in non-depressed patients. In conclusion, non-depressed PD patients are 20% more likely to fall for every one unit increase in the ESS measure of sleepiness.
Papillomavirus Infection Requires γ Secretase (2010)

The mechanism by which papillomaviruses breach cellular membranes to deliver their genomic cargo to the nucleus is poorly understood. Here, we show that infection by a broad range of papillomavirus types requires the intramembrane protease γ secretase. The γ-secretase inhibitor (S,S)-2-[2-(3,5-difluorophenyl)-acetylamino]-N-(1-methyl-2-oxo-5-phenyl-2,3-dihydro-1H-benzo[e][1,4]diazepin-3-yl)-propionamide (compound XXI) inhibits infection in vitro by all types of papillomavirus pseudovirions tested, with a 50% inhibitory concentration (IC(50)) of 130 to 1,000 pM, regardless of reporter construct and without impacting cellular viability. Conversely, XXI does not inhibit in vitro infection by adenovirus or pseudovirions derived from the BK or Merkel cell polyomaviruses. Vaginal application of XXI prevents infection of the mouse genital tract by human papillomavirus type 16 (HPV16) pseudovirions. Nicastrin and presenilin-1 are essential components of the γ-secretase complex, and mouse embryo fibroblasts deficient in any one of these components were not infected by HPV16, whereas wild-type and β-secretase (BACE1)-deficient cells were susceptible. Neither the uptake of HPV16 into Lamp-1-positive perinuclear vesicles nor the disassembly of capsid to reveal both internal L1 and L2 epitopes and bromodeoxyuridine (BrdU)-labeled encapsidated DNA is dependent upon γ-secretase activity. However, blockade of γ-secretase activity by XXI prevents the BrdU-labeled DNA encapsidated by HPV16 from reaching the ND10 subnuclear domains. Since prior studies indicate that L2 is critical for endosomal escape and targeting of the viral DNA to ND10 and that γ secretase is located in endosomal membranes, our findings suggest that either L2 or an intracellular receptor are cleaved by γ secretase as papillomavirus escapes the endosome.
Theo Murphy International Scientific Meeting between the UK and China on the chemistry and physics of functional materials (2014)

Single crystals of elemental or simple binary or ternary materials, such as Si, GaN or Nb3Sn, have been the basis of many past and current electronic, optoelectronic and energy technologies. However, there has been a recognition that in order to meet demanding future performance requirements and to
Reduction of crosstalk on printed circuit board using genetic algorithm in switching power supply (2001)

Crosstalk between printed circuit board (PCB) traces in switching power supplies may cause high electromagnetic interference emission. PCB layout plays an important part and a genetic algorithm (GA) is used to produce a layout with reduced crosstalk. A coupling index and a new way of representing a trace for the GA process is presented.
Clinical Application of the Food Insulin Index for Mealtime Insulin Dosing in Adults with Type 1 Diabetes: A Randomized Controlled Trial (2016)

In a 12-week pilot study, MAGE and postprandial glycemia following breakfast were significantly improved with FII counting versus CC, despite no significant differences in HbA1c.