gebhart/patrepeval huggingface dataset:
Nanoscale spatio-temporal diffusion modes measured by simultaneous confocal and STED imaging (2018)

Abstract The diffusion dynamics in the cellular plasma membrane provides crucial insights into the molecular interactions, organization and bioactivity. Fluorescence correlation spectroscopy combined with super-resolution stimulated emission depletion nanoscopy (STED-FCS) measures such dynamics with high spatial and temporal resolution and reveals nanoscale diffusion characteristics by measuring the molecular diffusion in conventional confocal mode and super-resolved STED mode sequentially. However, to directly link the spatial and the temporal information, a method that simultaneously measures the diffusion in confocal and STED modes is needed. Here, to overcome this problem, we establish an advanced STED-FCS measurement method; line interleaved excitation scanning STED-FCS (LIESS-FCS) which discloses the molecular diffusion modes at different spatial positions with a single measurement. It relies on fast beam-scanning along a line with alternating laser illumination that yields, for each pixel, the apparent diffusion coefficients for two different observation spot sizes (conventional confocal and super-resolved STED). We demonstrate the potential of the LIESS-FCS approach with simulations and experiments on lipid diffusion in model and live cell plasma membranes. We also apply LIESS-FCS to investigate the spatio-temporal organization of GPI-anchored proteins in the plasma membrane of live cells which interestingly show multiple diffusion modes at different spatial positions.
Characterization of the Receptor Binding Properties of Monoiodoinsulin Isomers and the Identification of Different Insulin Receptor Specificities in Hepatic and Extrahepatic Tissues (1983)

Insulin was iodinated with 125I using a modification of the lactoperoxidase method. This iodinated insulin mixture was then separated by HPLC, allowing studies to be performed on the pure monoiodoinsulin isomers labeled at the tyrosyl residuesat A14, B16, or B26. Binding studies in adipocytes revealed the relative affinity for these isomers to be 1.00:0.99:1.30 (A14, B16, and B26, respectively). The increase in binding affinity was shown to be due to a slower dissociation rate rather than an accelerated association rate. Receptor mediated internalization of the different iodinated isomers was assessed and these results showed that the isomers were internalized and degraded in proportion to their relative binding affinities. The biologic activity of the B26 isomer to stimulate adipocyte glucose oxidation was measured and showed that the B26 isomer had 30% greater biologic potency compared with native insulin, consistent with its 30% increase in binding potency. Thus, the B26 monoiodoinsulin isomer behaves as a superagonist. Binding studies using extrahepatic tissue (i.e., adipocytes, cultured human fibroblasts, IM-9 lymphocytes, and human placental membranes) consistently revealed that the relative binding affinity for the B26 isomer was always greater than the A14 isomer (1.29, 2.11,1.22, and 1.35, respectively) while the binding affinity of the B16 isomer was generally comparable to the A14 isomer. In hepatic tissue (whole cell, liver plasma membranes, and partially purified, solubilized liver receptors) binding studies illustrated that the B26 isomer consistently had a lower relative affinity when compared to the A14 isomer (0.75 : 0.69 : 0.77). In conclusion, (1) the B26 monoiodoinsulin isomer exhibits higher binding affinity and greater biologic activity in adipocytes than native insulin, and (2) the B26 monoiodoinsulin isomer has a higher binding affinity in extrahepatic tissues but exhibits a lower binding affinity in hepatic tissues when compared with the A14 monoiodoinsulin isomer, indicating a striking difference in functional specificity between the hepatic and the extrahepatic insulin receptor.
Relative strength of thermal and electrical spin currents in a ferromagnetic tunnel contact on a semiconductor (2015)

The relative magnitude of electrical and thermal spin currents is investigated for a ferromagnetic tunnel contact on a semiconductor. A direct quantitative comparison is made by simultaneously generating electrical and thermal spin currents of opposite sign and determining the compensation point at which the sum of both spin currents vanishes. This avoids the need to determine the magnitude of each spin current. Surprisingly, it is found that in a $\mathrm{C}{\mathrm{o}}_{70}\mathrm{F}{\mathrm{e}}_{30}/\mathrm{MgO}/\mathrm{Si}$ tunnel contact, the thermal spin current driven by a temperature difference of less than 1 K across the contact is comparable to the electrical spin current induced by a bias voltage of about 22 mV. This suggests that the thermal generation of spin current is more efficient than hitherto assumed and should be considered in the design and analysis of spintronic devices that use spin current or spin transfer torque.
Add-Drop Filter Incorporating a Mode-Conversion Cavity (2011)

We demonstrate a new add-drop filter architecture combining an asymmetric Y-branch mode conversion waveguide and a high index contrast shifted grating mode-conversion cavity. Measurements show add-drop functionality in a linear Fabry-Perot filter.
Technical Note: Packing Factors of Feed Products in Storage Structures (2008)

Experiments were conducted to measure the changes in bulk density of cracked corn, corn meal, soybean meal, cotton seed meal, and distillers dried grain (without solubles) when subjected to simulated overburden pressures. All materials were tested at two moisture content levels (approximately 8% and 12% w.b.) and seven pressures between 0 and 69 kPa (0 and 10 psi). A mathematical model was fitted to the data to predict the bulk density of each feed ingredient as a function of pressure and moisture content. These relationships were inserted into a previously developed computer model to predict ingredient packing within conventional storage structures based on Janssen's equation as a function of feed product type, moisture content of the material, friction characteristics of the bin wall material, material height, and bin diameter. Cracked corn experienced the smallest amount of packing (approximately 4.3% in a bin with a diameter of 1.8 m and a height of 1.8 m), while distillers dried grain (without solubles) had approximately 8.1% packing in the same sized bin. With a bin diameter of 5.5 m and a height of 5.5 m, distillers dried grain (without solubles) and cracked corn had a packing factor of 13.3% and 6.8%, respectively. As moisture content increased the amount of packing increased for all materials. The data presented can be used for inventory control and management.
Antitumor cells found in tumor-bearing mice given ubenimex. (1987)

Antitumor effector cells in spleens of tumor-bearing mice given ubenimex were investigated. The administration of ubenimex, starting 7 days after the tumor inoculation, was effective in inhibiting growth of IMC carcinoma. Spleen cells taken from these mice showed a marked suppression of the tumor growth by the WINN assay. The antitumor activity of spleen cells was reduced by treatment to remove T cells or NK cells, whereas spleen cell preparations enriched for T cells showed the strongest antitumor activity. Moreover, NK activity against YAC-1 cells was found in the spleen. These results indicate that the administration of ubenimex to IMC carcinoma-bearing mice generates cytotoxic T cells and NK cells in the spleen. The antitumor effect of ubenimex was not observed in X-ray-irradiated and in anti-asialo GM1 serum-treated mice.
The Authors' Reply (1990)

Journal Article The Authors' Reply Get access Ralph H. Hruban, M.D., Ralph H. Hruban, M.D. The Johns Hopkins Hospital Baltimore, Maryland Search for other works by this author on: Oxford Academic Google Scholar Jonathan I. Epstein, M.D. Jonathan I. Epstein, M.D. The Johns Hopkins Hospital Baltimore, Maryland Search for other works by this author on: Oxford Academic Google Scholar American Journal of Clinical Pathology, Volume 94, Issue 1, 1 July 1990, Page 118, https://doi.org/10.1093/ajcp/94.1.118a Published: 01 July 1990
IL-10 and IL-13 as B cell growth and differentiation factors. (1993)

B lymphocytes express at their surface the CD40 antigen which belongs to the NGF receptor superfamily. The crosslinking of the CD40 antigen using a mouse fibroblastic cell line expressing the human Fc receptor (Fc gamma RII/CDw32) and anti-CD40 monoclonal antibody induces resting B lymphocytes to enter a state of sustained proliferation. Addition of IL-4 or IL-13 result in the proliferation of human B cells and in the secretion of IgE following isotype switching. Addition of IL-10 permits limited cell proliferation but most importantly results in very high immunoglobulin production following differentiation of B cells into plasma cells. In response to IL-10, unseparated B cells cultured in the CD40 system produced the four IgG subclasses in ratio comparable to those observed in the serum. IL-10 induces naive B cells to secrete low but reproducible amounts of IgG and IgA. The combination of IL-10 and TGF beta induces naive B cells to secrete IgA1 and IgA2 as a consequence of isotype switching. The extracellular domain of CD40 binds with high affinity and high specificity to a ligand transiently expressed on activated T cells. This interaction of the CD40 antigen on B cells with its counter-structure on T cells represents a key step in T cell dependent B cell activation.
Gemeinschaft und Gesellschaft: Über die Schwierigkeiten einen Unterschied zu machen (2005)

Als Klassiker der Soziologie bewahrt der kulturelle Diskurs die Werkspur von Ferdinand Tönnies. Nach der von Frank Osterkamp durchgeführten Interpretation ist der einzelwissenschaftliche Soziologe Tönnies jedoch erst das Ergebnis der einsichtsvollen Selbstverwandlung eines systematisch arbeitenden, wissenschaftstheoretisch, erkenntnisanthropologisch und evolutionstheoretisch argumentierenden Philosophen. Die Rekonstruktion zeichnet das bisher ungemalte Bild eines der kreativen Forschungsphilosophen der Epochenschwelle vom 19. zum 20. Jahrhundert. Zug um Zug tritt das Spannungsgefüge einer Theorielandschaft hervor, die sich als Themenmatrix der Philosophie des 20. Jahrhunderts entpuppt. Ausgangspunkt ist eine problemorientierte Sichtung der Tönnies-Forschung. Im Zentrum der anschließenden Darlegungen stehen u. a. Tönnies' wissenschaftstheoretischer Konstruktivismus, das Prekärwerden der Stellung der Philosophie im Verhältnis zu den Wissenschaften, seine doppelparadigmatische "psychische Anthropologie", die Nachkonstruktion zweier Modellvarianten der Theorie von Gemeinschaft und Gesellschaft, eine bisher weitgehend außer acht gelassene Sprachtheorie und die weltbildgeschichtlich reinterpretierte Operationalisierung des Grundrisses zwischen einer atomar-nominalistischen und einer holistisch-prozessualen Erklärungsontologie moderner Wissenschaft
Outcomes of Iliac Crest Bone Marrow Aspirate Injection for the Treatment of Recalcitrant Non- Insertional Achilles Tendinitis (2016)

Category: Sports Introduction/Purpose: Non-insertional Achilles tendinitis is a common cause of posterior ankle and heel pain in active and sedentary patients. Though the majority of patients respond to first-line non-operative management including activity modification, immobilization, orthotics, and physical therapy with tendon stretching, there is no consensus for patients that fail these treatments. We evaluate the role of iliac crest bone marrow aspirate concentrate (BMAC) as a safe and effective treatment option for recalcitrant cases. Methods: A retrospective chart review was conducted of patients with greater than 12 months of non-insertional Achilles tendinitis symptoms despite appropriate conservative treatment. Each patient had BMA harvested from the iliac crest, concentrated by centrifugation, and then injected into the Achilles tendon. Symptoms were assessed using the Visual Analogue Scale (VAS) pain score, collected at the preoperative office visit and at 6 weeks, 12 weeks, and 24 weeks after the procedure. Co- morbidities, concurrent procedures, and complications were also recorded. Results: A total of 21 patients (22 feet) were treated with iliac crest BMA concentrate injections. Preoperatively, the average VAS pain score was 6.8 (SD 2.0). Postoperatively, the average VAS was 4.0 (SD 2.5) at 6 weeks, 2.7 (SD 2.1) at 12 weeks, and 2.2 (SD 2.1) at 24 weeks. At 24 weeks postoperatively, there was a statistically significant decrease VAS score from baseline (p &lt; 0.001), with a mean decrease by 4.6 (SD 3.1). There were no adverse effects reported at the surgical site or donor site morbidity at the iliac crest such as wound infection, hematoma, or persistent pain. Conclusion: Iliac crest BMAC appears to be a safe, effective, and potentially lasting treatment option for patients with intractable, non-insertional Achilles tendinitis. Patients demonstrated a statistically significant decrease in VAS pain score postoperatively with no complications at the donor or injection site.
Visualizing topical drug uptake with conventional fluorescence microscopy and deep learning (2020)

Mapping the uptake of topical drugs and quantifying dermal pharmacokinetics (PK) presents numerous challenges. Though high resolution and high precision methods such as mass spectrometry offer the means to quantify drug concentration in tissue, these tools are complex and often expensive, limiting their use in routine experiments. For the many topical drugs that are naturally fluorescent, tracking fluorescence emission can be a means to gather critical PK parameters. However, skin autofluorescence can often overwhelm drug fluorescence signatures. Here we demonstrate the combination of standard epi-fluorescence imaging with deep learning for the visualization and quantification of fluorescent drugs in human skin. By training a U-Net convolutional neural network on a dataset of annotated images, drug uptake from both high "infinite" dose and daily clinical dose regimens can be measured and quantified. This approach has the potential to simplify routine topical product development in the laboratory.
Oleuropein or rutin consumption decreases the spontaneous development of OA in Hartley guinea pig (2013)

peer reviewed
Elevated Pressure in the Left Renal Vein in Patients with Varicocele: Preliminary Observations (1980)

In 3 patients with varicocele a pressure difference was found between the left renal vein and inferior vena cava. No pressure difference was found between the right renal vein and inferior vena cava in these same patients. In a control group of 5 patients right and left renal veins and inferior vena cava pressures closely approximated each other. These preliminary findings are discussed and found to be in disagreement with current concepts of the pathogenesis of varicocele.
Scn8a Antisense Oligonucleotide Is Protective in Mouse Models of SCN8A Encephalopathy and Dravet Syndrome (2020)

Reduction of Scn8a transcript by 25 to 50% delayed seizure onset and lethality in mouse models of SCN8A encephalopathy and Dravet syndrome. Reduction of SCN8A transcript is a promising approach to treatment of intractable childhood epilepsies. Ann Neurol 2020;87:339-346.
Enhanced performance of AlN SAW devices with wave propagation along the 〈11−20〉 direction on c -plane sapphire substrate (2019)

Abstract This paper investigates the effect of acoustic wave propagation direction along the a -direction (〈11−20〉) and m -direction (〈1−100〉) on the frequency responses of c -plane AlN based surface acoustic wave (SAW) devices systematically. The experimental results indicate that the resonant frequency ( ), quality factor ( Q ), electromechanical coupling coefficient ( ), insertion loss and out-of-band rejection can be improved for the a -direction compared with the m -direction of an AlN based SAW resonator on sapphire. The Q is 1347 for a one-port SAW resonator along the a -direction, and the minimum insertion loss is 8.71 dB for a two-port SAW resonator along the a -direction. The insertion loss is 3.23 dB lower for the a -direction compared to the m -direction of the AlN film, which may be attributed to the larger acoustic power flow density. The is 45% higher for the a -direction compared to the m -direction of the AlN film, which may be attributed to the larger elastic constant. In addition, our finite element model simulation results reveal the C 11 and C 44 for the m -direction are 345 GPa and 102 GPa, while C 11 and C 44 for the a -direction is increased to 429 GPa and 128 GPa, respectively. Our work demonstrates that the a -direction is better than the m -direction of the AlN film for high performance SAW device applications.
The Effect of Heteropoly Acids on Stability of PFSA PEMs under Fuel Cell Operation (2007)

Membranes were cast from mixtures of the 3M perfluorinated sulfonic acid ionomer [side chain ] and various heteropoly acids (HPAs) at a 10 or doping level. Membrane electrode assemblies (MEAs) fabricated from these membranes were subjected to a fuel cell testing protocol from 70 to under relatively dry conditions, dew point of , to avoid leaching of the HPA. The most significant finding was that the more stable HPAs, , , and , reduce the rate of by over half and improve the power of the MEA by 9% under these conditions.
Context‐based ensemble classification for the detection of architectural distortion in a digitised mammogram (2019)

The problem of computer‐aided detection of architectural distortion (AD) in a digitised mammogram has been attempted in this manuscript. In examining a mammogram, the decision regarding a particular region of interest (RoI) is dependent on the appearance of the surrounding regions. However, in existing methods to detect AD the inference about an RoI is dependent on the appearance of this RoI alone. In addition, multiple radiologists infer the same mammogram in coming to a final decision about the mammogram. Contrary to popular ensemble classifiers like Adaboost and Random Forest, the authors propose an ensemble based method (imitating multiple radiologists by classifiers) for detecting AD such that the decision on a test RoI is dependent on the decisions of the surrounding RoIs in the proposed ensemble classifier. The proposed context‐based ensemble classifier has been validated on two mammographic databases. The proposal shows promising results in both the databases.
Supplementary Table 1 from Combination Immunotherapy after ASCT for Multiple Myeloma Using MAGE-A3/Poly-ICLC Immunizations Followed by Adoptive Transfer of Vaccine-Primed and Costimulated Autologous T Cells (2023)

&lt;p&gt;PDF file - 54KB, Adverse Events Table.&lt;/p&gt;
Fractal Distributions of Reservoir Properties and Their Use in Reservoir Simulation (1994)

Abstract Geostatistics has become a popular way to distribute reservoir properties between wells. One of the geostatistical methods being used is fractal geostatistics. Most porosity well logs have been found to have a fractal character. An analysis of vertical and horizontal logs, core photos, and outcrop photos has led to a rather simple model to describe porosity and permeability distributions. This representation has been tested in reservoir simulation of a mature waterflood and found to match production history with very little history matching. Fractal distributions were found to require much less history matching than classical layer cake models. Conoco is now actively applying this new technology to a number of its reservoirs.
Abstract 13264: Varying Ventilation Strategies During Cardiac Arrest Has Minimal Impact on Hemodynamics (2016)

Introduction: Prolonged duty cycle ventilation and hyperventilation are associated with reduced venous return during CPR. Meanwhile, the 30:2 compressions-ventilation method requires pauses in compressions, which could also negatively affect hemodynamics. We investigated the effect of varying ventilation strategies on hemodynamics in a swine model of cardiac arrest. Hypothesis: Continuous ventilations with a short duty cycle, as compared to longer duty cycles and standard 30:2 ventilation, would be associated with improvements in hemodynamics. Methods: CPR was performed on eight domestic swine (~30 kg) using standard physiological monitoring. Blood flow was measured in the inferior vena cava (IVC). Ventricular fibrillation (VF) was electrically induced. Mechanical chest compressions (CC) were started after four minutes of VF. CC were delivered at a rate of 100 compressions per minute and a depth of 2” for a total of 12 min. Animals were intubated and mechanically ventilated throughout the experiment. Three ventilation strategies were tested: 1) continuous ventilator-driven ventilation (CV-DV) at a rate of 6 breaths per minute and a 0.20 duty cycle, 2) CV-DV at a rate of 6 breaths per minute and a 0.50 duty cycle, and 3) standard 30:2 at a 0.20 duty cycle. All tidal volumes were calculated as 7 ml/kg. PEEP was set to zero for all ventilations. Results: Across 12 minutes of CPR, aortic pressure and coronary perfusion pressure was greater with CV-DVs of 0.20 and 0.50 duty cycle as compared to 30:2 (Table). Overall net flow in the IVC was not different among ventilation strategies. Conclusions: As compared to the 30:2 method, CV-DVs at 0.20 and 0.50 duty cycles produced small increases in blood pressures but no changes in venous return. Further research is needed to determine if hemodynamics can be improved by synchronizing continuous ventilations to CC and if this association reflects elimination of the interruptions in chest compressions.
Ausgedehnte akute Lungenembolie: Fragmentationsbehandlung mit dem Pigtail-Rotationskatheter (1996)

A 58-year-old patient with extensive pulmonary embolism complicated by cardiogenic shock and contraindications to thrombolysis was treated by mechanical embolus fragmentation using a rotatable pigtail catheter system. Considerable recanalization of the left pulmonary arteries was rapidly achieved with relative ease of instrumentation. Subsequently, the hemodynamic condition stabilized.
Apparent interleukin 2 (IL-2) inhibitory activity of human serum is due to rapid killing of IL-2-dependent mouse cells. (1987)

A number of investigators have reported that human serum inhibits the proliferation of IL-2-dependent mouse cells in IL-2 bioassays, but the mechanism of inhibition has not been carefully examined. We noticed that IL-2-dependent mouse cells (CTLL-2) are killed within 30 min in the presence of a 1/10 dilution of human serum. However, CTLL-2 cells totally deprived of IL-2 did not begin to die until at least 6 h in culture. Thus, even complete inhibition of IL-2 by human serum could not account for the rapid cytotoxicity caused by human serum. Since humans have 'natural' antibodies which react with mouse cells, it seemed possible that the cytotoxicity was due to antibody/complement-mediated cell lysis. This was supported by the observation that EDTA (at a concentration sufficient to inhibit complement) protected CTLL-2 cells from the cytotoxic effects of human sera from four normal donors. In addition, preincubation of CTLL-2 cells with heat-inactivated human sera at 4 degrees C rendered them much more susceptible to lysis with rabbit complement than cells which were preincubated with complete culture medium. The cytotoxicity of human serum is not limited to IL-2-dependent mouse cells but was also observed with EL4 and Ag8.653 cells as well as normal splenocytes. The cytotoxic effect of human serum was lost upon removal of IgM, but not upon removal of IgG. These results strongly suggest that the inhibition of proliferation of IL-2-dependent mouse cells by human serum is due to antibody/complement-mediated lysis of those cells. In addition, non-heat-inactivated human serum did not inhibit the IL-2-mediated proliferation of human PHA blasts, indicating that there is no inherent inhibitory activity in human serum apart from the cytotoxic effect on xenogeneic cells. Thus the reported IL-2 inhibitory activity of whole human serum is probably not biologically relevant.