gebhart/patrepeval huggingface dataset:
Pitting Corrosion Resistance of Anodized Aluminum-Copper Alloy Processed by Severe Plastic Deformation (2008)

The effect of equal-channel angular pressing (ECAP) on the pitting corrosion resistance of anodized Al-Cu alloy was investigated by electrochemical techniques in a solution containing 0.2 mol/L of AlCl3 and also by surface analysis. The time required before initiating pitting corrosion of anodized Al-Cu alloy was longer with ECAP than without, indicating improvement in the pitting corrosion resistance by application of ECAP. Second phase precipitates were present in Al-Cu alloy matrix and the size of these precipitates was greatly decreased by application of ECAP. The precipitates composed of Si and Al-Cu-Si-Fe-Mn were not oxidized during anodization, and the anodic oxide film were absent at the boundary between the normal oxide films and these impurity precipitates. The pitting corrosion of anodized Al-Cu alloy occurred preferentially around these precipitates, the improvement of pitting corrosion resistance of anodized Al-Cu alloy by ECAP appears to be attributable to a decrease in the size of precipitates, which act as origins of pitting corrosion.
Integrin-dependent Antagonism of Tamoxifen Therapy through Transcriptional Replacement: Establishing a Basis for a New Combined Therapy (2004)

Abstract : Accruing evidence suggests that integrin-dependent cell attachment signaling, and estrogen hormonal response are closely interconnected. For example, studies by us and others of the BCAR proteins (BCAR1 and BCAR3: (1, 2)) have indicated that these proteins physically associate with each other, and function both in signal transduction relevant to integrin stimulation, and in mediation of Tamoxifen (Tam) resistance. The goal of this proposal was to explore the interrelationship between integrin signaling, cell attachment status, and Tam resistance. A specific hypothesis was that activation of integrin signaling reduces the Tam-dependent inhibition of essential estrogen-dependent transcription. In particular, the proposal sought to explore how the formation of organized three-dimensional structures (spheroids) by metastasizing tumor cells, which greatly enhances their resistance to treatment with a number of drugs (reviewed in (4), might modulate Tam resistance and the estrogen-dependent transcriptional program. As described below, we found that spheroids unexpectedly did not result in increased Tam resistance. However, we did find that manipulation of expression of BCAR1 resulted in changes in the transcription of estrogen-regulated genes, suggesting the initial hypothesis of an integrin-estrogen connection at the level of transcription is worth further investigation.
GAIM fusions are therapeutic candidates for peripheral amyloidosis (2019)

We have previously described the discovery and development of GAIM (general amyloid interaction motif)-Ig (immunoglobulin) fusions as therapeutic candidates for neurodegenerative indications [1,2]....
Filtre passe-bande d’ordre trois à guide d'ondes intégré au substrat creux multicouche en bande Ka (2022)

International audience
Supplementary table 2 from A Novel Epigenetic Signature for Early Diagnosis in Lung Cancer (2023)

&lt;p&gt;Primers for pyrosequencing and priority list.&lt;/p&gt;
Rate Adaptive Flexible Optical Transceivers (2018)

Client rates are adapted in 25 Gb/s steps to allow the fine-granularity tradeoff between noise and throughput. Advanced hardware mitigates the effects of noise and nonlinearity.
Editorial: Ear-Centered Sensing: From Sensing Principles to Research and Clinical Devices (2020)

The human ears are an attractive location for bio-signal acquisition. Heart rate, respiratory rate, skin conductance, eye blink, and eye motion signals, as well as the electrical activity from muscles and the brain can be recorded from the ear. Moreover, the ears provide a discreet and natural anchoring point for placing the necessary wearable hardware, thereby reducing the visibility of integrated devices. In this Research Topic, we define ear-centered sensing as monitoring physiological signals with sensors located in the earcanal (intra-aural), in the pinna, or around the ear (circum-aural). Ear-centered sensing allows data recording over extended periods of time in everyday situations with little disturbance for the users. As the ear is an unconventional place for monitoring these physiological measures, it is necessary to gain a better understanding of the signals and to characterize the signals relative to the conventional measurement methods.
Exploring nuclear spin systems by relayed magnetization transfer (1982)

A method is given for identifying families of signals belonging to the same network of coupled spins in NMR. The method exploits the existence of a coupling network to prove the connectivity between 2 remote nuclei A and X in the AMX system. Two or more consecutive steps are employed where transverse magnetization is relayed from nucleus to nucleus along a path defined by a sequence of resolved couplings. The spectrum of crotonaldehyde is discussed. [on SciFinder (R)]
Targeted Fluorogenic Cyanine Carbamates Enable In Vivo Analysis of Antibody-Drug Conjugate Linker Chemistry (2021)

Antibody-drug conjugates (ADCs) are a rapidly emerging therapeutic platform. The chemical linker between the antibody and the drug payload plays an essential role in the efficacy and tolerability of these agents. New methods that quantitively assess cleavage efficiency in complex tissue settings could provide valuable insights into the ADC design process. Here we report the development of a near-infrared (NIR) optical imaging approach that measures the site and extent of linker cleavage in mouse models. This approach is enabled by a superior variant of our recently devised cyanine carbamate (CyBam) platform. We identify a novel tertiary amine-containing norcyanine, the product of CyBam cleavage, that exhibits dramatically in-creased cellular signal due to improved cellular permeability and lysosomal accumulation. The resulting cyanine lysosome-targeting carbamates (CyLBams) are ~50X brighter in cells, and we find this strategy is essential for high-contrast in vivo targeted imaging. Finally, we compare a panel of several common ADC linkers across two antibodies and tumor models. These studies indicate that cathepsin-cleavable linkers provide dramatically higher tumor activation relative to hindered or non-hindered disulfides – an observation that is only apparent with in vivo imaging. This strategy enables quantitative comparisons of cleavable linker chemistries in complex tissue settings with implications across the drug delivery landscape.
The reduced responsiveness of cultured bovine parathyroid cells to extracellular Ca2+ is associated with marked reduction in the expression of extracellular Ca(2+)-sensing receptor messenger ribonucleic acid and protein. (1995)

PTH secretion from dispersed bovine parathyroid cells maintained in culture becomes progressively less responsive to changes in the extracellular Ca2+ concentration (Ca2+o) over several days. We have now investigated whether this change in secretory control is associated with alterations in the expression of the Ca2+o-sensing receptor (BoPCaR) recently cloned from bovine parathyroid, which plays a central role in Ca2+o-regulated PTH secretion. BoPCaR messenger RNA levels dropped rapidly in cultured bovine parathyroid cells, as assessed by Northern analysis, decreasing by 78% within 18 h and remaining low for at least 4 days. The level of receptor protein decreased to a comparable extent (approximately 72-82%) after 3-4 days in culture, as determined by immunocytochemistry with specific antibodies directed at the extracellular domain of the receptor. The half-time for the reduction in receptor protein (approximately 2 days) was considerably longer, however, than that for BoPCaR messenger RNA, but was comparable to that for the loss of sensitivity of PTH secretion to Ca2+o. Indeed, there was a close linear correlation between maximal suppressibility of PTH secretion and the intensity of staining for the receptor protein (r = 0.88; P = 0.004). We conclude that alterations in the expression of BoPCaR could explain much of the reduced responsiveness of cultured bovine parathyroid cells to Ca2+o.
Enrichment of low-frequency functional variants revealed by whole-genome sequencing of multiple isolated European populations (2017)

The genetic features of isolated populations can boost power in complex-trait association studies, and an in-depth understanding of how their genetic variation has been shaped by their demographic history can help leverage these advantageous characteristics. Here, we perform a comprehensive investigation using 3,059 newly generated low-depth whole-genome sequences from eight European isolates and two matched general populations, together with published data from the 1000 Genomes Project and UK10K. Sequencing data give deeper and richer insights into population demography and genetic characteristics than genotype-chip data, distinguishing related populations more effectively and allowing their functional variants to be studied more fully. We demonstrate relaxation of purifying selection in the isolates, leading to enrichment of rare and low-frequency functional variants, using novel statistics, DVxy and SVxy. We also develop an isolation-index (Isx) that predicts the overall level of such key genetic characteristics and can thus help guide population choice in future complex-trait association studies.
Electrogram morphology discriminators in implantable cardioverter defibrillators: A comparative evaluation (2020)

Proprietary morphology discriminators show important differences in their ability to discriminate SVT. How much this impact the overall discrimination process remains to be investigated.
Supplementary Figure Legends and Materials and Methods and Tables 1-5 from Progesterone Receptor Signaling in the Microenvironment of Endometrial Cancer Influences Its Response to Hormonal Therapy (2023)

&lt;p&gt;PDF - 155K, Supplementary Table 1: Antibodies used for FACS sorting. Supplementary Table 2: Antibodies used for IHC. Supplementary Table 3: Primers used for QPCR. Supplementary Table 4: Antibodies used for Western blotting.Supplementary Table 5: Primers used for Methylation Specific. PCR&lt;/p&gt;
Development of terbinafine solid lipid nanoparticles as a topical delivery system (2012)

To resolve problems of long treatment durations and frequent administration of the antifungal agent terbinafine (TB), solid lipid nanoparticles (SLNs) with the ability to load lipophilic drugs and nanosize were developed. The SLNs were manufactured by a microemulsion technique in which glyceryl monostearate (GMS), glyceryl behenate (Compritol(®) 888; Gattefossé), and glyceryl palmitostearate (Precirol(®) ATO 5; Gattefossé) were used as the solid lipid phases, Tween(®) and Cremophor(®) series as the surfactants, and propylene glycol as the cosurfactant to construct ternary phase diagrams. The skin of nude mice was used as a barrier membrane, and penetration levels of TB of the designed formulations and a commercial product, Lamisil(®) Once™ (Novartis Pharmaceuticals), in the stratum corneum (SC), viable epidermis, and dermis were measured; particle sizes were determined as an indicator of stability. The optimal SLN system contained a <5% lipid phase and >50% water phase. The addition of ethanol or etchants had no significant effect on enhancing the amount of TB that penetrated the skin layers, but it was enhanced by increasing the percentage of the lipid phase. Furthermore, the combination of GMS and Compritol(®) 888 was able to increase the stable amount of TB that penetrated all skin layers. For the ACP1-GM1 (4% lipid phase; Compritol(®) 888: GMS of 1:1) formulation, the amount of TB that penetrated the SC was similar to that of Lamisil(®) Once™, whereas the amount of TB of the dermis was higher than that of Lamisil(®) Once™ at 12 hours, and it was almost the same as that of Lamisil(®) Once™ at 24 hours. It was concluded that the application of ACP1-GM1 for 12 hours might have an efficacy comparable to that of Lamisil(®) Once™ for 24 hours, which would resolve the practical problem of the longer administration period that is necessary for Lamisil(®) Once™.
Analyses of a Holocene soil/loess sequence in Southern Siberia (2013)

We conducted a high-resolution study of a unique Holocene sequence of wind-blown sediments and buried soils in Southern Siberia, far from marine environment influences. This was accomplished in order to assess the difference between North Atlantic marine and in-land climate variations. Relative wind strength was determined by grain size analyses of different stratigraphic units. Petromagnetic measurements were performed to provide a proxy for the relative extent of pedogenesis. An age model for the sections was built using the radiocarbon dating method. The windy periods are associated with the absence of soil formation and relatively low values of frequency dependence of magnetic susceptibility (FD), which appeared to be a valuable quantitative marker of pedogenic activity. These events correspond to colder intervals which registered reduced solar modulation and sun spot number. Events, where wind strength was lower, are characterized by soil formation with high FD values. Spectral analysis of our results demonstrates periodic changes of 1500, 1000 and 500 years of relatively warm and cold intervals during the Holocene of Siberia. We presume that the 1000 and 500 year climatic cycles are driven by increased solar insolation reaching the Earth surface and amplified by other still controversial mechanisms. The 1500 year cycle associated with the North Atlantic circulation appears only in the Late Holocene. Three time periods -- 8400-9300 years BP, 3600-5100 years BP, and the last ~250 years BP - correspond to both the highest sun spot number and the most developed soil horizons in the studied sections
Cyclohexylmethylpiperidinyltriphenylpropioamide:  A Selective Muscarinic M3 Antagonist Discriminating against the Other Receptor Subtypes (2002)

To discover a highly selective M(3) antagonist, a combinatorial library was prepared. The library was designed to identify a novel structural class of M(3) antagonists by exploring the spatial arrangement of the pharmacophores in known M(3) antagonists. After the evaluation of 1000 library members, a potent M(3) antagonist, 14a (K(i) = 0.31 nM), with novel structural features was identified. Compound 14a showed high selectivity for M(3) receptors over the other muscarinic receptor subtypes (M(1)/M(3) = 380-fold, M(2)/M(3) = 98-fold, M(4)/M(3) = 45-fold, M(5)/M(3) = 120-fold).
Electromagnetic simulation of paired-wire and u-shaped resonator metamaterial composites for terahertz and optical frequencies (2007)

In a previous work, the present authors have demonstrated that the requisite electric-type response in a double-negative material can be realized by properly orientating single SRRs, whose particle size is small compared to the wavelength. Since it is necessary to orient these electric SRRs so that they are orthogonal to the k-vector of the incident wave, it is possible to deposit them on the same substrate that is used to print the paired wires. For operation in the mid-infrared frequency range, the SRRs should be replaced by U- shaped (horse-shoe) resonators, and a unit cell of infrared metamaterial can be realized by combining paired wires with U-shaped resonators that also exhibit bianisotropy in common with the SRRs. In this paper we use the finite difference time domain (FDTD) method to investigate the frequency response of a composite wire-pair and U- shaped resonators, forming a unit cell, to see if they produce the desired metamaterial characteristics we seek.
Effect of Dual Impeller‐Sparger Geometry on the Hydrodynamics and Mass Transfer in Stirred Vessels (2004)

Abstract The understanding of the effect of impeller‐sparger configurations on gas dispersion and mass transfer is very important to improve the performance of gas/liquid contactor systems. The influence of the impeller positions, the upper turbine diameter, the sparger ring diameter and its location in regard to the lower impeller on the power consumption, the volumetric mass‐transfer coefficient and the overall oxygen transfer efficiency were studied in a nonstandard curved bottomed reactor with an agitated system with dual disk style turbines. In the range of the gas flow rates studied, the most efficient impeller‐sparger arrangement for the oxygen transfer is the impeller system with turbines of different diameters located at C = 0.25 and IC = 0.5, and with the sparger of smaller diameter than the lower impeller settled below the impeller. A new model to estimate the k L a with an average relative error of 8 %, which takes the reactor operation conditions and the influence of the impeller‐sparger geometry into account, was also proposed.
Efficacy and safety of live recombinant BCG vaccines. (1995)

BCG has a long history of safe use in humans and is one of the best adjuvants known. The use of newer production methods may further reduce the risk of adverse side-effects. Early results with experimental animals have shown BCG to be an effective live recombinant delivery vehicle for several foreign vaccine antigens. Additional refinements to the safety and efficacy of the recombinant BCG vaccine vehicle are under development.
SMS design method 3D: Calculating multiple free form surfaces from the optical prescription (2003)

A method of design of free-form optical surfaces is presented. Given 2 input wavefronts and other 2 output wavefronts, the method provides an optical system with 2 free-form surfaces that deflects the rays of the input wavefronts into the rays of the corresponding output wavefronts and vice versa.
Effects of Gears, Bearings, and Housings on Gearbox Transmission Shafting Resonances (2010)

Gear noise, comprised of tones centered around gear meshing frequencies, is transmitted through driveshafts, bearings, and transmission housings. Gear noise transmission is amplified, sometimes strongly, by modes of vibration of the shafting. The shafts of the input and output gears vibrate like beams at low to mid frequencies. The resonance frequencies and vibration patterns of these beam-like modes depend strongly on the shaft boundary conditions, which in turn depend on the impedances of the bearings, and of the transmission housing. In this paper, we present simulated and measured modes of the input and output shafts of a single stage spur gear system mounted in a rectangular metal gearbox. The impedances of the gears, bearings, and housing are added in sequence and the effects on the fundamental mode shapes and frequencies are shown. The bearing impedances have the largest effects on the shafting modes, increasing the resonance frequencies significantly, along with spreading the frequencies of similar mode types. Including the housing impedances at the bearing locations reduces slightly the spread of shaft mode frequencies.
725. Correction of Laminin-5-Deficient Junctional Epidermolysis Bullosa by Transplantation of Genetically Modified Epidermal Stem Cells. A Phase-I Clinical Trial (2006)

Mutations in genes encoding the laminin-5 heterotrimer, a key component of the epidermal-dermal junction, cause junctional epidermolysis bullosa (JEB), a severe and often fatal skin adhesion defect. Epidermal stem cells isolated from patients affected by β3 chain-deficient JEB were transduced with a retroviral vector expressing a β3 cDNA, and used to generate uniformly transduced cultured skin implants. The transgene was steadily expressed for >160 cell doublings in culture, leading to restoration of normal laminin 5 levels, assembly of functional hemidesmosomes, and full phenotypic correction. Cloning and sequencing of vector integrations showed that <20 stem cells are responsible for long-term maintenance of a transplantable skin culture. A phase-I clinical trial started in October 2005, aimed at proving the safety of the transduction/transplantation procedure, and analyzing persistence of transgene expression and long-term survival of transduced stem cells. The first patient was a 30-yr-old male affected by non-lethal JEB, carrying a null mutation in one LAMB3 allele and a point mutation (E212K) in the other one. The mutation affects the assembly of the laminin-5 heterotrimer, present at residual levels (<5%) in vitro and in vivo. Six genetically modified, cultured epidermal sheets of 100 sq cm were transplanted on both legs after removal of the outer skin layer using a minimally invasive technique. The procedure was well tolerated, and the patient discharged after five days. Engraftment was completed after 10 days, and transplanted skin remained stable on both legs in the absence of blistering or inflammation for the duration of the follow-up (4 months at the time of writing). 3-mm punch biopsies were taken 1 and 3 months after transplantation, and analyzed for vector presence by quantitative PCR and for protein expression by immunohistochemistry. A vector signal compatible with full transduction of the transplanted epidermis was observed at both time points. Synthesis and assembly of normal levels of heterotrimeric laminin-5 and α6β4 integrin was observed at the level of the basal lamina in all biopsies, together with the development of a firmly adherent, fully differentiated epidermis. Epidermal stem cells (p64+) were detected in the basal layer of the transplanted skin in normal numbers. These data show that gene therapy of JEB by transplantation of genetically corrected stem cells is feasible, and leads to full phenotypic correction of the adhesion defect in vivo. Safety studies are under way, which include detection or humoral or cytotoxic immune responses against laminin-5, and ex vivo cloning and sequencing of the integrated proviruses.
Grating Couplers and Ring Resonator in Aluminum Nitride (2012)

We report on the use of aluminum nitride (AlN) as an integrated photonic material. One-dimensional grating couplers, waveguides and rings are fabricated in 400 nm polycrystalline AlN films. Single couplers exhibit −6.6 dB peak insertion loss.
Transgene containment via maternal inheritance and male sterility in GM crops. (2004)

The potential of genetically modified (GM) crops to transfer foreign genes through pollen to related plant species has been recognized as a potential environmental concern. Until the environmental impact of novel genes on indigenous crops and weeds is thoroughly investigated, practical and regulatory considerations might require the adoption of gene containment approaches for future generations of GM crops. To date, most molecular approaches with potential for controlling gene flow among crops and weeds have focused on maternal inheritance, male sterility, and seed sterility.This presentation will focus on the use of maternal inheritance and cytoplasmic male sterility for transgene containment. Because no single strategy will be broadly applicable to all crop species, a combination of more than one approach might prove most effective for engineering failsafe mechanisms for the next generation of GM crops. Concerns about the environmental impact of GM crops currently limit their widespread acceptance around the world. Many of these concerns focus on the premise that such transfer could potentially result in the emergence of “superweeds ” resistant to herbicides or the introduction of undesired traits into related crop plants. Gene flow depends upon several factors, including the specific crop, its location, the presence of out-crossing wild relatives/sexually compatible crops, the competitive nature (advantages and disadvantages) of the introduced trait, and the environmental consequences of neutral traits. Two