gebhart/patrepeval huggingface dataset:
Preclinical characterization of alginate‐poly‐L‐lysine encapsulated HepaRG for extracorporeal liver supply (2020)

We recently demonstrated that HepaRG cells encapsulated into 1.5% alginate beads are capable of self-assembling into spheroids. They adequately differentiate into hepatocyte-like cells, with hepatic features observed at Day 14 post-encapsulation required for external bioartificial liver applications. Preliminary investigations performed within a bioreactor under shear stress conditions and using a culture medium mimicking acute liver failure (ALF) highlighted the need to reinforce beads with a polymer coating. We demonstrated in a first step that a poly-l-lysine coating improved the mechanical stability, without altering the metabolic activities necessary for bioartificial liver applications (such as ammonia and lactate elimination). In a second step, we tested the optimized biomass in a newly designed perfused dynamic bioreactor, in the presence of the medium model for pathological plasma for 6 h. Performances of the biomass were enhanced as compared to the steady configuration, demonstrating its efficacy in decreasing the typical toxins of ALF. This type of bioreactor is easy to scale up as it relies on the number of micro-encapsulated cells, and could provide an adequate hepatic biomass for liver supply. Its design allows it to be integrated into a hybrid artificial/bioartificial liver setup for further clinical studies regarding its impact on ALF animal models.
A 160 GHz Pulsed Radar Transceiver in 65 nm CMOS (2014)

This paper presents a 160 GHz center frequency pulsed 65 nm CMOS transceiver for short range radar applications. Four phased array transceivers were implemented in a single chip with antennas implemented in a BGA package. The implemented transmitter is capable of producing pulses of 100 ps widths ( >20 GHz RF bandwidth) at a 160 GHz carrier frequency. The measured effective isotropic radiated power (EIRP) is 18.8 dBm for continuous wave outputs. The analog beam forming receiver achieves an overall gain of 42.5 dB, -14 dBm IP1dB, 7 GHz bandwidth, and a noise figure of 22.5 dB. The sliding window time-dilation baseband relaxes the output data rate and subsequent digital processing requirements. Fine grained duty cycling reduces power dissipation. The entire chip consumes 2.2 W from 1.2/1.4 V supplies in a 65 nm digital CMOS process.
In Vivo Manipulation of Endogenous Metallothionein with a Monoclonal Antibody Enhances a T-Dependent Humoral Immune Response (2001)

Metallothionein (MT) is a small stress response protein that can be induced by exposure to heavy metal cations, oxidative stressors, and acute phase cytokines that mediate inflammation. In previous experiments, we have shown that exogenous MT can affect cell proliferation, macrophage and cytotoxic T lymphocyte function, and humoral immunity to T-dependent antigens. In the studies described here, we have explored the effect of a monoclonal anti-MT antibody (clone UC1MT) on the role that endogenous MT plays in the humoral immune response. In vivo injection of UC1MT significantly increased the humoral response to simultaneous challenge with ovalbumin (OVA). In contrast, mice immunized with OVA in the presence of an isotype-matched antibody control (MOPC 21) showed no change in the anti-OVA humoral response. The predominant anti-OVA response that was enhanced by UC1MT treatment was the IgG(1) response; the IgG(2a) anti-OVA response was not altered by UC1MT treatment. UC1MT treatment increased the numbers of IgG anti-OVA secreting cells as measured by ELISPOT assay, suggesting that blocking the effects of MT synthesized during the immune response augments the differentiation of antigen-specific plasma cells. The percentages of T and B cells in the spleens of animals from each treatment group were not significantly different, suggesting that this regimen of UC1MT treatment does not significantly affect hematopoiesis, but rather alters antigen-induced differentiation of lymphocytes. These observations are compatible with previous results from our laboratory that suggest that endogenous MT synthesized during the normal immune response or as a consequence of toxicant exposure suppresses in vivo immune function. In light of the fact that significant amounts of MT can be synthesized during toxicant exposure, manipulation of MT levels with an anti-MT antibody may ultimately represent an important therapeutic approach to the treatment of immune dysfunctions that result from toxicant exposure.
Ginger Compound [6]-Shogaol and Its Cysteine-Conjugated Metabolite (M2) Activate Nrf2 in Colon Epithelial Cells in Vitro and in Vivo (2014)

In this study, we identified Nrf2 as a molecular target of [6]-shogaol (6S), a bioactive compound isolated from ginger, in colon epithelial cells in vitro and in vivo. Following 6S treatment of HCT-116 cells, the intracellular GSH/GSSG ratio was initially diminished but was then elevated above the basal level. Intracellular reactive oxygen species (ROS) correlated inversely with the GSH/GSSG ratio. Further analysis using gene microarray showed that 6S upregulated the expression of Nrf2 target genes (AKR1B10, FTL, GGTLA4, and HMOX1) in HCT-116 cells. Western blotting confirmed upregulation, phosphorylation, and nuclear translocation of Nrf2 protein followed by Keap1 decrease and upregulation of Nrf2 target genes (AKR1B10, FTL, GGTLA4, HMOX1, and MT1) and glutathione synthesis genes (GCLC and GCLM). Pretreatment of cells with a specific inhibitor of p38 (SB202190), PI3K (LY294002), or MEK1 (PD098059) attenuated these effects of 6S. Using ultra-high-performance liquid chromatography-tandem mass spectrometry, we found that 6S modified multiple cysteine residues of Keap1 protein. In vivo 6S treatment induced Nrf2 nuclear translocation and significantly upregulated the expression of MT1, HMOX1, and GCLC in the colon of wild-type mice but not Nrf2(-/-) mice. Similar to 6S, a cysteine-conjugated metabolite of 6S (M2), which was previously found to be a carrier of 6S in vitro and in vivo, also activated Nrf2. Our data demonstrated that 6S and its cysteine-conjugated metabolite M2 activate Nrf2 in colon epithelial cells in vitro and in vivo through Keap1-dependent and -independent mechanisms.
Review focus on inorganic nitrite and nitrate in cardiovascular health and disease (2010)

This editorial refers to the following review articles in the issue which are part of a Review Focus on Inorganic Nitrite and Nitrate: M. Gilchrist et al .[7][1], pp. 492–498 ; J.W. Calvert[10][2], pp. 499–506; R.P. Patel et al .[12][3], pp. 507–515; Q. Yu et al .[16][4], pp. 516–524; J.O. Lundberg et al .[17][5], pp. 525–532; C.B. Patillo et al .[19][6], pp. 533–541; B.S. Zuckerbraun et al .[23][7], pp. 542–552; A. Cauwels and P. Brouckaert[24][8], pp. 553–559. 

Reviews and original articles in this focused issue of Cardiovascular Research address the topic of inorganic nitrite and nitrate in cardiovascular pathophysiology. It is well accepted that nitric oxide (NO) is a critical regulator of cardiovascular health that is routinely depleted during vascular disease and dysfunction.1,2 Since the original discovery of NO as a mediator of cardiovascular health, thousands of studies have focused on mechanisms of NO generation and function, largely addressing NO synthase (NOS) activity and NO-dependent signalling responses (e.g. soluble guanylate cyclase and nitrosylation reactions).3,4 From the beginning, a physiological role for inorganic nitrite or nitrate in mediating the biological effects of NO was briefly considered; however, early experimental data did not support a compelling role for these molecules in participating in NO physiology. Over the last decade, a handful of investigators have challenged this dogma through studies re-examining the relationship between NO, nitrite, and nitrate and have elucidated the newly described nitrite/nitrate/NO endocrine system. In the current review papers, the authors discuss how nitrite/nitrate serve as an important reservoir for NO and detail how under specific conditions nitrite/nitrate can be reduced back to NO to increase its bioavailability independent of classical NOS activity. The importance and mechanisms of this unique feature of nitrite/nitrate anion are further detailed with respect to cardiovascular health and potential therapeutic uses.

Historical and new, emerging evidence …

 [1]: #ref-7
 [2]: #ref-10
 [3]: #ref-12
 [4]: #ref-16
 [5]: #ref-17
 [6]: #ref-19
 [7]: #ref-23
 [8]: #ref-24
Robot assisted gait training with active leg exoskeleton (ALEX) (2008)

Gait training of stroke survivors can help in retraining their muscles and improving their gait pattern. Robot assisted gait training (RAGT) was developed for stroke survivors using ALEX and force-field controller, which use assist-as-needed paradigm for rehabilitation. In this paradigm undesirable gait motion is resisted and assistance is provided towards the desirable motion. The force-field controller achieves this paradigm by applying forces at the foot of the subject. Two stroke survivors participated in a 15-day gait training study each with ALEX. The results show that by the end of the training the gait pattern of the patients was improved towards healthy subjects gait pattern. Improvement is seen as increase in the size of the patients’ gait pattern, increase in knee and ankle joint excursions and increase in their walking speed on the treadmill.
Correction to “Improved Kinetics of n-Butane Oxidation to Maleic Anhydride: The Role of Byproducts” (2022)

ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionNEXTORIGINAL ARTICLEThis notice is a correctionCorrection to "Improved Kinetics of n-Butane Oxidation to Maleic Anhydride: The Role of Byproducts"Mauritio MüllerMauritio MüllerMore by Mauritio Müllerhttps://orcid.org/0000-0002-0647-5993, Martin KutscherauerMartin KutscherauerMore by Martin Kutscherauer, Sebastian BöckleinSebastian BöckleinMore by Sebastian Böcklein, Gerhard MestlGerhard MestlMore by Gerhard Mestl, and Thomas Turek*Thomas TurekMore by Thomas Turekhttps://orcid.org/0000-0002-7415-1966Cite this: Ind. Eng. Chem. Res. 2022, 61, 38, 14402Publication Date (Web):September 19, 2022Publication History Received10 September 2022Published online19 September 2022Published inissue 28 September 2022https://pubs.acs.org/doi/10.1021/acs.iecr.2c03265https://doi.org/10.1021/acs.iecr.2c03265correctionACS PublicationsCopyright © 2022 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views613Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (893 KB) Get e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Bacillus anthracis produces membrane-derived vesicles containing biologically active toxins (2010)

Extracellular vesicle production is a ubiquitous process in Gram-negative bacteria, but little is known about such process in Gram-positive bacteria. We report the isolation of extracellular vesicles from the supernatants of Bacillus anthracis, a Gram-positive bacillus that is a powerful agent for biological warfare. B. anthracis vesicles formed at the outer layer of the bacterial cell had double-membrane spheres and ranged from 50 to 150 nm in diameter. Immunoelectron microscopy with mAbs to protective antigen, lethal factor, edema toxin, and anthrolysin revealed toxin components and anthrolysin in vesicles, with some vesicles containing more than one toxin component. Toxin-containing vesicles were also visualized inside B. anthracis -infected macrophages. ELISA and immunoblot analysis of vesicle preparations confirmed the presence of B. anthracis toxin components. A mAb to protective antigen protected macrophages against vesicles from an anthrolysin-deficient strain, but not against vesicles from Sterne 34F2 and Sterne δT strains, consistent with the notion that vesicles delivered both toxin and anthrolysin to host cells. Vesicles were immunogenic in BALB/c mice, which produced a robust IgM response to toxin components. Furthermore, vesicle-immunized mice lived significantly longer than controls after B. anthracis challenge. Our results indicate that toxin secretion in B. anthracis is, at least, partially vesicle-associated, thus allowing concentrated delivery of toxin components to target host cells, a mechanism that may increase toxin potency. Our observations may have important implications for the design of vaccines, for passive antibody strategies, and provide a previously unexplored system for studying secretory pathways in Gram-positive bacteria.
L'abolition de la joyeuse entrée de Brabant (18 juin 1789): le coup d'état manqué de Joseph II (1995)

info:eu-repo/semantics/published
Spatio-temporal filtering methods for enhancement and separation of speech signals (2013)

In this paper, we give an overview of the background for, the ideas behind, and the challenges to be addressed in the project "Spatio-Temporal Filtering Methods for Enhancement and Separation of Speech Signals," which is funded by the Villum Foundation. The project aims at addressing the problem of enhancing and separating speech signals from noisy mixtures, a problem also known as the cocktail party problem. It aims at exploring new ways of solving this problem by generalizing a new class of optimal temporal filtering methods for periodic signals to multiple microphones, resulting in so-called spatio-temporal filtering methods that are controlled by two parameters, the direction-of-arrival and the fundamental frequency. These filters are optimal in that they let the signal of interest pass undistorted while everything else is attenuated as much as possible. Unlike state-of-the-art methods, they do not require knowledge of the statistics of noise and interfering speech signals, something that is especially important when dealing with non-stationary noise.
Recurrent breast cancer: stereotaxic localization for fine-needle aspiration biopsy. Work in progress. (1992)

The efficacy of stereotaxic localization for fine-needle aspiration biopsy in the detection of recurrent cancer manifested as calcifications on mammograms was evaluated in 43 patients that had been treated with local resection and radiation therapy. Six patients had malignant aspirates and one had an atypical aspirate; examination of the surgical specimens revealed all seven of these to be malignant. Thirteen patients underwent surgical biopsies, the results of which were malignant in seven and benign in six. The remaining 30 patients were followed up with mammography. The follow-up mammograms were obtained at 6-month intervals and demonstrated no change in appearance. On the basis of this initial experience, stereotaxic localization for aspiration biopsy offers the potential to accurately distinguish benign from malignant lesions.
Object cosegmentation (2011)

Cosegmentation is typically defined as the task of jointly segmenting “something similar” in a given set of images. Existing methods are too generic and so far have not demonstrated competitive results for any specific task. In this paper we overcome this limitation by adding two new aspects to cosegmentation: (1) the “something” has to be an object, and (2) the “similarity” measure is learned. In this way, we are able to achieve excellent results on the recently introduced iCoseg dataset, which contains small sets of images of either the same object instance or similar objects of the same class. The challenge of this dataset lies in the extreme changes in viewpoint, lighting, and object deformations within each set. We are able to considerably outperform several competitors. To achieve this performance, we borrow recent ideas from object recognition: the use of powerful features extracted from a pool of candidate object-like segmentations. We believe that our work will be beneficial to several application areas, such as image retrieval.
Cu wire and Pd-Cu wire package reliability and molding compounds (2012)

Cu wire is drastically replacing Au wire due to surge of Au price. However, Cu wire package has poorer humidity reliability than Au wire package. Although Pd coated Cu wire package could show better humidity reliability than Cu wire, it is still worse than Au. Enough information regarding failure mechanism was not available. For failure analysis, x-section has been widely used to identify the Cu/Al IMC after failure. However, the x-section is the results of corrosion reaction and doesn't show the IMC status before corrosion. Therefore, the failure mechanism could not be estimated precisely. We used chemical model simulation to predict what kinds of IMC could be created after wire bonding, then which IMC could be corroded more easily during HAST. The Desorption energy was used to estimate reactivity between specified Cu/Al IMC and chlorine ion. The simulation suggested that the formation of Cu rich and Cu poor Cu/Al IMC and the Cu rich IMC was estimated to be corroded by chlorine ion. These chemical model simulations are the effective way to have fundamental understanding of the mechanism of Cu/Al IMC corrosion. Furthermore, chemical model simulation for Pd coated Cu wire was done to explore the effect of Pd existence and distribution of Pd in Cu/Al IMC. Dispersed Pd contributed to create new IMC of Cu/Al/Pd instead of easily corroded Cu rich Cu/Al IMC. Cu and Al diffusion and also Cl ion diffusion were inhibited by Pd at surface. Even Cl ion catching effect by Pd is also discussed. To improve humidity reliability performance with Cu wire, we developed new ion trapper using chemical model simulation technique. Developed molding compounds with the ion trapper showed significant improvement at bias HAST with Cu wire, which was even better than conventional Cu wire compatible molding compounds.
Small-angle options of the upgraded ultrasmall-angle x-ray scattering beamline BW4 at HASYLAB (2006)

We present the upgrade and present status of the ultrasmall-angle x-ray scattering (USAXS) beamline BW4 at the Hamburg Synchrotronstrahlungslabor. In order to extend the accessible scattering vector range, new small-angle setups have been established, making use of the high flux and small divergence of BW4. In standard transmission geometry using a beam size of B=400×400μm2 (horizontal×vertical), typical small-angle resolution ranges from dmax=90to650nm, depending on sample-to-detector distance. Additionally a new microfocus option has been established. This microfocus option allows reducing the sample size by one order of magnitude. Using parabolic beryllium compound refractive lenses, a new standard beam size of B=65×35μm2 (horizontal×vertical) can be provided. The μ-SAXS resolution is as high as dmax=150nm. Using μ-SAXS in combination with grazing incidence (μ-GISAXS) on a standard noble metal gradient multilayer, we prove the feasibility of μ-GISAXS experiments at a second generation source.
The noradrenergic profile of plasma metanephrine in neuroblastoma patients is reproduced in xenograft mice models and arise from PNMT downregulation (2021)

Metanephrines (MNs; normetanephrine (NMN), metanephrine (MN) and methoxytyramine (MT)) detected in urine or plasma represent the best biomarker for neuroblastoma (NB) diagnosis, however the metabolism of both catecholamine (CAT) and MNs remains enigmatic in NB. Using patient-derived xenograft (PDX) models derived from primary NB cells, we observed that the plasma levels of MNs in NB-PDX-bearing mice were comparable as in patients. Interestingly, murine plasma displayed an elevated fraction of glucuronidated forms of MNs relative to human plasma where sulfonated forms prevail. In tumors, the concentration ranges of MNs and CAT and the expression levels of the main genes involved in catecholamine metabolism were similar between NB-PDX and human NB tissues. Likewise, plasma and intratumoral profiles of individual MNs, with increased levels of MT and NMN relative to MN, were also conserved in mouse models as in patients. We further demonstrated the downregulation of the Phenylethanolamine N-Methyltransferase gene in NB biopsies and in NB-PDX explaining this biochemical phenotype, and giving a rational to the low levels of epinephrine and MN measured in NB affected patients. Thus, our subcutaneous murine NB-PDX models not only reproduce the phenotype of primary NB tumors, but also the metabolism of catecholamine as observed in patients. This may potentially open new avenues in preclinical studies for the follow up of novel therapeutic options for NB through the quantification of plasma MNs.
Chemical Approaches to Three-Dimensional Semiconductor Photonic Crystals (2001)

We review recent efforts to make three-dimensional semiconductor photonic crystals using self-assembly techniques. These approaches, which utilize a synthetic opal as a template to shape the semiconductor material (see Figure), provide a simple and inexpensive alternative to lithographic methods. Since the resulting structures can, in principle, have a complete photonic bandgap – a property that would allow ultimate control over the flow of light – these materials may have serious implications for modern photonics.
Field Validation of a Dynamic Model for an MFL ILI Tool in Gas Pipelines (2010)

Movements of pigs in gas pipelines are subject to more stringent parameters than that in liquid pipelines, predominantly due to the compressibility of gas. This is accentuated when the pig has to negotiate an upward inclination in the section of the pipeline, where the gravity force due to its weight can compromise the driving pressure drop across it. On a downward slope, a pig can accelerate to a velocity higher than the maximum required for the proper operation the instrumentation (which is typically around 5 m/s). On the other hand, in-line inspection tools often face challenges at wall thickness transitions or bends. The ability to accurately predict the functional performance of pigs is vital in the design and operation of pipelines and their associated pigging programs. The present paper provides a general formulation for the motion of pigs in an inclined pipeline section, taking into account effects of gas properties, wall friction, by-pass flow for speed control, differential pressure across the pig, seal efficiency, and gap flows, among other parameters. Comparison between model prediction and actual data from pigging a 158 km NPS 18 gas pipeline on TransCanada’s pipeline system in Alberta, Canada is presented. The elevation profile along this pipeline contains both positive (upward) and negative (downward) slopes. This is a lateral line which features 28 gas receipt points along the line, all were feeding in gas during the pigging program. Good agreement between model prediction and field data is demonstrated within ± 8% of St. Deviation. Example of a problem occurring at wall thickness transition at a valve section is demonstrated by a sudden stop of an MFL tool followed by a shootout at a higher velocity once the pressure is built up behind it.