gebhart/patrepeval huggingface dataset:
Involvement of the C‐terminal end of the prostrate‐specific antigen in a conformational epitope: characterization by proteolytic degradation of monoclonal antibody‐bound antigen and mass spectrometry (2001)

Prostate-specific antigen (PSA), a 237-amino acid glycoprotein, encoded by the hKLK3 gene, is widely used as a serum marker for the diagnosis and management of prostate cancer. We report here the localization of a conformational epitope recognized by the anti-total PSA monoclonal antibody (mAb) 11E5C6, by proteolytic degradation of mAb-bound antigen followed by mass spectrometric analyses of the peptides generated. These two technologies, combined with molecular display, allowed the identification of amino acid residues contained within three different peptides distant on the PSA sequence, but close in the PSA three-dimensional structure, that may be part of the mAb 11E5C6 epitope. The last four C-terminal amino acid residues are included in this epitope, as well as certain other C-terminal residues between Y225 and T232. The involvement of the PSA C-terminal end in the mAb 11E5C6 epitope was confirmed by western blotting experiments with the recombinant protein proPSA-RP1, resulting from the cloning of an alternative transcript of the hKLK3 gene, in which the PSA C-terminal end was deleted and replaced by another sequence. Although the anti-total PSA mAb 5D5A5 used as a control bound proPSA-RP1, mAb 11E5C6 did not. The requirement of the C-terminal end for the recognition by mAb 11E5C6 may be useful for the discrimination of PSA-related forms.
Carbopeptides: carbohydrates as potential templates for de novo design of protein models (2000)

De novo design of proteins has evolved into a powerful approach for studying the factors governing protein folding and stability. Among the families of structures frequently studied is the 'four-helix bundle' in which four alpha-helical peptide strands, linked by loops, form a hydrophobic core. Assembly of protein models on a template has been suggested as a way to reduce the entropy of folding. Here we describe the potential use of a carbohydrate as such a template. The monosaccharide D-galactose was per-O-acylated with (Nbeta-Fmoc-betaAla)2O to give a penta-substituted derivative, which was converted to the corresponding glycosyl bromide and used for the glycosylation of 4-hydroxymethylbenzoic acid pentafluorophenyl ester (HMBA-OPfp). The beta-glycosidic carbohydrate template (Nbeta-Fmoc-3Ala)4-beta-D-Galp-(1-O)-MBA-OPfp thus obtained was coupled to a PAL-PEG-PS resin and simultaneously extended at the four arms to yield, after cleavage from the solid support, a carbopeptide with four identical peptide strands. Extension of this concept to, for example, synthesis of novel multiple antigenic peptides (MAPs) and synthesis of carbohydrate clusters can be easily envisioned. The ability to efficiently synthesize such structures sets the stage for further studies to test whether the carbohydrate templates do indeed nucleate folding.
Associations between circulating cell-free mitochondrial DNA, inflammatory markers, and cognitive and physical outcomes in community dwelling older adults (2023)

In a community dwelling cohort of older adults, there are cross-sectional and longitudinal associations between ccf-mtDNA and sTNFR1 with impaired physical and cognitive function and increased hazard of death. This work suggests a role for long ccf-mtDNA as a blood-based marker predictive of future physical decline.
Obesity: a paradox in the mortality of the elderly (2013)

Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
Structure Sensitive Reaction Channels of Molecular Hydrogen on Silicon Surfaces (2001)

The ability of the Si(001) surface to adsorb H2 molecules dissociatively increases by orders of magnitude when appropriate surface dangling bonds are terminated by H atoms. Through molecular beam techniques the energy dependent sticking probability at different adsorption sites on H-precovered and stepped surfaces is measured to obtain information about the barriers to adsorption, which decrease systematically with an increase in coadsorbed H atoms. With the help of density functional calculations for interdimer adsorption pathways, this effect is traced back to the electronic structure of the different adsorption sites and its interplay with local lattice distortions.
[Non-pharmacological treatments for osteoarthritis: unavoidable and essential]. (2019)

The optimal treatment of osteoarthritis requires the combination of pharmacological and non-pharmacological treatments. The non-pharmacological treatments recommended by the medical and scientific associations are information, education, weight management (especially in case of overweight) and physical activity. Antalgic and anti-inflammatory pharmacological treatments should be used to facilitate the recovery and regular physical activity that is central in the management of osteoarthritis patients. It should include aerobic cardio-respiratory, resistance, flexibility and neuromuscular reprogramming and be associated with cognitive-behavioral therapies. Health professionals have to promote the activity and to inform their patients about the benefits, not only about the symptoms of osteoarthritis, but also about their overall health.
Scheduling-based test-case generation for verification of multimedia SoCs (2006)

Multimedia SoCs are characterized by a main controller that directs the activity of several cores, each of which is in charge of a stage in the processing of a media stream. The verification of these SoCs is a significant challenge due to time-to-market constraints and system complexity. We present a novel approach to system-level, random test case generation for multimedia SoCs, and a tool, called SoCVer, that implements this approach. We use the SoC's main controller point of view for controlling the flow of data in the SoC. Test case generation is done by allocating processing tasks to the various cores and determining which core processes which data item at what time. Solving these scheduling problems allows SoCVer to generate software for the SoC's main controller; this software coordinates and synchronizes the operations of all the cores on the chip without the need for the real operational software. We demonstrate the use of SoCVer using a DVD player SoC.
Cost Effective and High Performance 28nm FPGA with New Disruptive Silicon-Less Interconnect Technology (SLIT) (2014)

This paper introduces the first comprehensive demonstration of new disruptive innovation technology comprising multiple Xilinx patent-pending innovations for highly cost effective and high performance Xilinx FPGA, which is so called stack silicon-less interconnect technology (SLIT) that provides the equivalent high-bandwidth connectivity and routing design-rule as stack silicon interconnect (SSI) technology at a cost-effective manner. We have successfully demonstrated the overall process integration and functions of our new SLIT-employed package using Virtex® -7 2000T FPGA product. Chip-to-Wafer stacking, wafer level flux cleaning, micro-bump underfilling, mold encapsulation are newly developed. Of all technology elements, both full silicon etching with high etch selectivity to dielectric/fast etch rate and wafer warpage management after full silicon etching are most crucial elements to realize the SLIT technology. In order to manage the wafer warpage after full Si removal, a couple of knobs are identified and employed such as top reinforcement layer, micro-bump underfill properties tuning, die thickness/die-to-die space/total thickness adjustments. It's also discussed in the paper how the wafer warpage behaves and how the wafer warpge is managed. New SLIT module shows excellent warpage characteristics of only −30 μm ~ −40 μm at room temperature for 25 mm × 31 mm in size and +20 μm ~ +25 μm at reflow temperature. Thermal simulation results shows that thermal resistance of new SLIT package is almost comparable to that of standard 2000T FCBGA package using TSV interposer with standard heat sink configuration and air wind condition. The reliability assessment is now under the study.
Molecular cloning and functional characterization of a type-I neurotensin receptor (NTR) and a novel NTR from the bullfrog brain (2005)

Neurotensin (NT) is a tridecapeptide that functions as a neurotransmitter and neuromodulator in the nervous system. To date, three different types of NT receptor (NTR), NTR1, NTR2 and NTR3, have been identified only in mammalian species. In the present study we isolated the cDNAs for an NTR1 and a novel NTR in the bullfrog brain, designated bfNTR1 and bfNTR4 respectively. bfNTR1 and bfNTR4 encode 422- and 399-amino acid residue proteins respectively. bfNTR1 has a 64% amino acid identity with mammalian NTR1, and 34-37% identity with mammalian NTR2. bfNTR4 exhibits 43% and 45-47% identity with mammalian NTR1 and NTR2 respectively. Both receptors are mainly expressed in the brain and pituitary. bfNTR1 triggers both CRE-luc, a protein kinase A (PKA)-specific reporter, and c-fos-luc, a PKC-specific reporter, activities, indicating that bfNTR1 can activate PKA- and PKC-linked signaling pathways. However, bfNTR4 appears to be preferentially coupled to the PKA-linked pathway as it induces a higher CRE-luc activity than c-fos-luc activity. bfNTRs exhibit different pharmacological properties as compared with mammalian NTRs. Mammalian NTR1 but not NTR2 responds to NT, whereas both bfNTR1 and bfNTR4 show a high sensitivity to NT. SR 48692 and SR 142948A, antagonists for mammalian NTR1 but agonists for mammalian NTR2, function as antagonists for both bfNTR1 and bfNTR4. In conclusion, this report provides the first molecular, pharmacological and functional characterization of two NTRs in a non-mammalian vertebrate. These data should help to elucidate the phylogenetic history of the G protein-coupled NTRs in the vertebrate lineage as well as the structural features that determine their pharmacological properties.
Isolation of HIV-1-Neutralizing Mucosal Monoclonal Antibodies from Human Colostrum (2012)

These novel HIV-neutralizing mAbs isolated from a mucosal compartment provide insight into the ability of mucosal B cell populations to produce functional anti-HIV antibodies that may contribute to protection against virus acquisition at mucosal surfaces.
<title>Wide format image manipulation and compression in a printing environment</title> (2002)

We investigate the task of wide format still image manipulation and compression, within the framework of a wide format document data path. For such systems, the constraints are put on performance and cost: the use of data compression aims at reducing both storage cost and the transfer times, which are critical for wide format printing systems. Nevertheless, different factors reduce the overall system performance and usability; using inadequate compression algorithms for an inadequate document content -- that can mix text, graphics and photographs --minimizes its global usefulness. Other factors limit its usability, as the non-compatibility of compressed data-stream with basic image transformations or the pixel encoding route mostly in a raster order, that do not allow random image access. In our article, we survey the adequation of some of the existing standards and compressed file formats, with respect to the constraints of the large format document systems
Clinical Application of Cine-MRI in the Visual Assessment of Mitral Regurgitation Compared to Echocardiography and Cardiac Catheterization (2012)

Cine MRI offers a reasonable alternative to both Doppler echocardiography and, to a lesser extent, cardiac catheterization for visually assessing the severity of mitral regurgitation with specific visual parameters during routine clinical cardiac MRI.
.beta.-Tosylethylamine: A Useful Reagent for Preparation of N-Protected Amides, Carbamates, and Related Compounds. Application to Synthesis of .beta.-Lactams (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT.beta.-Tosylethylamine: A Useful Reagent for Preparation of N-Protected Amides, Carbamates, and Related Compounds. Application to Synthesis of .beta.-LactamsDarren DiPietro, Robert M. Borzilleri, and Steven M. WeinrebCite this: J. Org. Chem. 1994, 59, 20, 5856–5857Publication Date (Print):October 1, 1994Publication History Published online1 May 2002Published inissue 1 October 1994https://pubs.acs.org/doi/10.1021/jo00099a006https://doi.org/10.1021/jo00099a006research-articleACS PublicationsRequest reuse permissionsArticle Views558Altmetric-Citations23LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
ChemInform Abstract: The Synthesis of Aminopyridines: A Method Employing Palladium‐ Catalyzed Carbon‐Nitrogen Bond Formation. (1997)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
An examination of the longitudinal acoustic mode of polyethylene crystals (1983)

Abstract Chain lengths have been calculated from the peak positions of the longitudinal acoustic modes (LAM) of polyethylene single crystals grown at several different temperatures. The data are consistent with other experimental results when the crystalline elastic modulus is taken to be 3.6 × 1012 dyn/cm2. However, this is true only if the vibrations of the crystalline chain segments are unaffected by the presence of folds and cilia on the crystal surface. The LAMs of several crystal preparations were also deconvoluted with the first-order LAM of C94H190 in order to remove instrumental broadening. The band shapes of the deconvoluted spectra are consistent with the idea that the LAM of polyethylene crystals is composed of two contributions: One due to chain stems in 110 sectors and a second due to chains in 200 sectors.
A study of MRI-guided diffuse fluorescence molecular tomography for monitoring PDT effects in pancreas cancer (2009)

Over the last several decades little progress has been made in the therapy and treatment monitoring of pancreas adenocarcinoma, a devastating and aggressive form of cancer that has a 5-year patient survival rate of 3%. Currently, investigations for the use of interstitial Verteporfin photodynamic therapy (PDT) are being undertaken in both orthotopic xenograft mouse models and in human clinical trials. In the mouse models, magnetic resonance (MR) imaging has been used as a measure of surrogate response to Verteporfin PDT; however, MR imaging alone lacks the molecular information required to assess the metabolic function and growth rates of the tumor immediately after treatment. We propose the implementation of MR-guided fluorescence tomography in conjunction with a fluorescently labeled (IR-Dye 800 CW, LI-COR) epidermal growth factor (EGF) as a molecular measure of surrogate response. To demonstrate the effectiveness of MR-guided diffuse fluorescence tomography for molecular imaging, we have used the AsPC-1 (+EGFR) human pancreatic adenocarcinoma in an orthotopic mouse model. EGF IRDye 800CW was injected 48 hours prior to imaging. MR image sequences were collected simultaneously with the fluorescence data using a MR-coupled diffuse optical tomography system. Image reconstruction was performed multiple times with varying abdominal organ segmentation in order to obtain a optimal tomographic image. It is shown that diffuse fluorescence tomography of the orthotopic pancreas model is feasible, with consideration of confounding fluorescence signals from the multiple organs and tissues surrounding the pancreas. MR-guided diffuse fluorescence tomography will be used to monitor EGF response after photodynamic therapy. Additionally, it provide the opportunity to individualize subsequent therapies based on response to PDT as well as to evaluate the success of combination therapies, such as PDT with chemotherapy, antibody therapy or even radiation.
Market leadership by example: Government sector energy efficiency in developing countries (2002)

Government facilities and services are often a country’s largest energy users, and major purchasers of energy-using equipment. In developing as well as industrial countries, government “leadership by example ” can be a powerful force to shift the market toward energy efficiency, complementing other elements of a national energy efficiency strategy. Benefits from more efficient energy management in government facilities and operations include lower energy bills, reduced greenhouse gas emissions, less demand on electric utility systems, and reduced dependence on imported oil. Even more significantly, the government sector’s buying power and example to others can generate broader demand for energy-efficient products and services, creating entry markets for domestic suppliers and stimulating competition in providing high-efficiency products and services. Despite these benefits, government sector actions have often lagged behind other energy efficiency policies. This is especially true in developing countries and transition economies – with a few exceptions discussed in this paper – even though the public sector share of total energy use in these countries may be at least as large as that in industrial economies. This paper summarizes work in progress to inventory current programs and policies for government sector energy efficiency in developing countries. We describe successful case studies from Mexico’s implementation of public sector energy management, as a possible model for other developing countries. We describe how these policies, starting at the federal level, are now being extended to state and local agencies in Mexico.
Incisive Structure−Spectroscopic Correlation in Oligothiophenes Functionalized with (±) Inductive/Mesomeric Fluorine Groups:  Joint Raman and DFT Study (2005)

This paper presents a combined experimental Raman and density functional theory (DFT) study of a series of oligothiophenes with variable pi-core lengths and substituted at the alpha,omega- and beta,beta'-terminal positions with perfluorohexyl, perfluorohexylcarbonyl, perfluoroarene, and cyano groups. The study covers the neutral and the electrochemically oxidized samples. The spectra have been assigned with the help of B3LYP/6-31G** calculations and interpreted by the predictions of effective conjugation coordinate (ECC) theory. Direct relationships between the bond length alternation (BLA) parameters and the Raman wavenumbers of the two most important bands of the spectra have been outlined showing the collective character of their electronic and vibrational properties. It is found that the topology of the thienyl electronic structure is not uniform along the conjugated core with the external rings more aromatic, whereas for the innermost part the pi-electron delocalization is greater and extends with the increment of chain length. Perfluorohexyl substitution finely tunes the electronic properties via negative inductive effects. The beta,beta' isomers exhibit larger conformational distortion, which strongly modifies the mean conjugation length. Oxidation provokes electronic structure quinoidization spreading over the whole system but more marked in the central part of the pi-core. The Raman features associated to quinoidization in the oxidized species have been interpreted in relation to the oligomer core length and the substitution regiochemistry.
Hematopoietic prostaglandin D synthase is expressed in microglia in the developing postnatal mouse brain (2003)

Hematopoietic prostaglandin D synthase (HPGDS) is a PGD(2)-synthesizing enzyme and is expressed in antigen-presenting cells, mast cells, and other immunocompetent cells. We here report the HPGDS expression in microglia and the migration pathway of microglia in the developing mouse brain as detected by HPGDS immunohistochemistry. Expression of HPGDS mRNA peaked at postnatal day (PND) 10, decreased gradually thereafter, and reached a plateau at PND 20. The mRNAs for target molecules of PGD(2), i.e., DP receptor (DPR) and CRTH2 receptor, showed developmental profiles overlapped to that of HPGDS. Most of the HPGDS(+) cells at PND 10 had morphological characteristics of ameboid microglia and gave positive immunostaining with microglia-specific markers such as RCA-1, F4/80, or ER-MP12. These specific markers became less detectable later on, but HPGDS was still expressed even in resting microglia. Thus, HPGDS is a useful marker for investigation of microglial development. Spaciotemporal evaluation of microglial development and migration with HPGDS immunostaining revealed the following three major possible migration pathways of microglia in the postnatal brain: from the lateral ventricle via subventricular zones to brain parenchyma; from the leptomeninges around the cerebellopontine angle to the cerebellar white matter; and from the overlying leptomeninges to the hippocampus, basal forebrain, and brainstem.
A Convenient Synthesis of 1-Alkyl-4-oxo-1,4-dihydrocinnolines (1986)

Synthese par hydrolyse basique des produits d'alkylation (par des halogeno-1 alcanes) de triphenylphosphoranylidene-3 3H-cinnolinones-4
Preparation and characterization of volatile alkaline-earth metal complexes with multiply coordinated aminoalkoxide ligands (2001)

The aminoalkoxide complexes Sr(amak)2 (1) and Ba(amak)2 (2) were prepared by treatment of a multiply-chelating fluorinated aminoalcohol ligand HOC(CF3)2CH2N(CH2CH2OMe)2, (amak)H, with the Group 2 metal reagents Sr(OPri)2 and BaH2 respectively. Single crystal X-ray diffraction studies indicate that complex 1 possesses an 8-coordinate distorted bicapped octahedral geometry with all O and N atoms coordinated to the central Sr cation. However, complex 2 adopts a unique 10-coordinate bicapped square antiprismatic structure, of which the coordination number is increased by two fluorine-to-barium dative interactions. Variable temperature 19F NMR studies show the existence of two inter-convertible isomers in solution and their possible molecular structures are proposed according to their structures in the solid-state. Preliminary investigation suggests that these complexes are good CVD source reagents for depositing SrF2 and BaF2 thin films.