gebhart/patrepeval huggingface dataset:
[Leucine flux: total flux, oxidative catabolism, protein synthesis and breakdown during development in the rat]. (1981)

Total leucine flux (flux into or out of the free leucine pool) and its different components --oxidative catabolism, protein synthesis and protein degradation--were studied in male rats weighing 160 and 300 g and infused intravenously for 6 h with L-(14C) leucine. Plasma L-(14C) leucine specific activity was measured after 4 and 6 h of infusion. The excretion rate of 14CO2 was estimated during infusion. Total leucine flux and oxidative catabolism flux were calculated from the specific radioactivity in the plasma at plateau value. Rates of protein synthesis were calculated from the total flux after subtracting the proportion oxidized. Protein breakdown flux was estimated by the difference between total flux and dietary intake flux. Total leucine flux (mumoles/h/100 g body weight) was not significantly lower at 300 than at 160 g. Oxidation accounted for about 10 p. 100 of the leucine flux; this proportion was significantly larger at 300 than at 160 g. A comparison of protein synthesis flux between the two groups of tats, for the same value of total flux, showed a decrease of leucine utilization for protein synthesis in the older animals. The quantity of leucine provided by protein breakdown was comparatively more important at 300 than at 160 g. Protein deposition fell by about 50 p. 100 between these two weights.
Microscopic and spectroscopic investigation of MoS2 nanotubes/P3HT nanocomposites (2013)

Blends of conductive polymer poly(3-hexylthiophene) (P3HT) and size controlled MoS2 nanotubes (NTs) were prepared focusing on procedures which do not alter the electrical and optical properties of nanotubes, i.e., without surfactants. After disassembly of as-grown hedgehog-like self-assemblies of the MoS2 NTs, relatively homogeneous dispersions of the NTs in P3HT were prepared. Thin films of these blends were prepared via spin coating. The NTs were found efficiently wetted by the P3HT and nearly completely immersed in the films. The surface structure was composed of pure P3HT lamellae. Photoluminescence spectra taken on P3HT/MoS2 NT thin films revealed a quenching effect, which depends on the concentration of NTs. Results of optical microscopy and spectroscopy investigations, scanning tunnelling microscopy and scanning electron microscopy studies are shown.
Use of Passenger Vehicles by Older People with Disabilities (1999)

The goal of this study was to identify the range of problems faced by frail older people in using private passenger vehicles and to identify how assistive technology and selection of vehicles may reduce those problems. Research activities included a review of existing research, a review of assistive technology for vehicle use, four focus group sessions, and a survey of 101 older people with disabilities. The findings of the research indicated that frail older people had significant problems both as drivers and as passengers. Although they are reluctant to self-report driving problems, they did report many problems entering and exiting vehicles. Forty percent of those surveyed reported difficulty getting their legs in and out. The study identified many ways that occupational therapists can assist frail older people and their caregivers in using passenger vehicles. It also identifies gaps in research, vehicle design, and assistive technology that need to be addressed.
Dense wavelength multiplexing of 1550 nm QKD with strong classical channels in reconfigurable networking environments (2008)

To move beyond dedicated links and networks, quantum communications signals must be integrated into networks carrying classical optical channels at power levels many orders of magnitude higher than the quantum signals themselves. We demonstrate transmission of a 1550-nm quantum channel with up to two simultaneous 200-GHz spaced classical telecom channels, using ROADM (reconfigurable optical &lt;1dd drop multiplexer) technology for multiplexing and routing quantum and classical signals. The quantum channel is used to perform quantum key distribution (QKD) in the presence of noise generated as a by-product of the co-propagation of classical channels. We demonstrate that the dominant noise mechanism can arise from either four-wave mixing or spontaneous Raman scattering, depending on the optical path characteristics as well &lt;1S the classical channel parameters. We quantity these impairments and discuss mitigation strategies.
Cationic antimicrobial peptides activate a two‐component regulatory system, PmrA‐PmrB, that regulates resistance to polymyxin B and cationic antimicrobial peptides in Pseudomonas aeruginosa (2003)

The two-component regulatory system PhoP-PhoQ of Pseudomonas aeruginosa regulates resistance to cationic antimicrobial peptides, polymyxin B and aminoglycosides in response to low Mg2+ conditions. We have identified a second two-component regulatory system, PmrA-PmrB, that regulates resistance to polymyxin B and cationic antimicrobial peptides. This system responds to limiting Mg2+, and is affected by a phoQ, but not a phoP mutation. Inactivation of the pmrB sensor kinase and pmrA response regulator greatly decreased the expression of the operon encoding pmrA-pmrB while expression of the response regulator pmrA in trans resulted in increased activation suggesting that the pmrA-pmrB operon is autoregulated. Interposon mutants in pmrB, pmrA, or in an intergenic region upstream of pmrA-pmrB exhibited two to 16-fold increased susceptibility to polymyxin B and cationic antimicrobial peptides. The pmrA-pmrB operon was also found to be activated by a number of cationic peptides including polymyxins B and E, cattle indolicidin and synthetic variants as well as LL-37, a component of human innate immunity, whereas peptides with the lowest minimum inhibitory concentrations tended to be the weakest inducers. Additionally, we showed that the putative LPS modification operon, PA3552-PA3559, was also induced by cationic peptides, but its expression was only partially dependent on the PmrA-PmrB system. The discovery that the PmrA-PmrB two-component system regulates resistance to cationic peptides and that both it and the putative LPS modification system are induced by cationic antimicrobial peptides has major implications for the development of these antibiotics as a therapy for P. aeruginosa infections.
A physical layer perspective on current and next-generation passive optical networks (2008)

This tutorial will focus on physical layer issues associated with passive optical networks. Corresponding technologies of present and future deployments will also be discussed.
Development of Advanced Surface Enhancement Technology for Decreasing Wear and Corrosion of Equipment Used for Mineral Processing (2006)

Equipment wear is a major concern in the mineral processing industry, which dramatically increases the maintenance cost and adversely affects plant operation efficiency. In this research, novel surface treatment technologies, High Density Infrared (HDI) and Laser Surface Engineering (LSE) surface coating processes were developed for the surface enhancement of selected mineral processing equipment. Microstructural and mechanical properties of the coated specimens were characterized. Laboratory-simulated wear tests were conducted to evaluate the tribological performance of the coated components. Test results indicate that the wear resistance of ASTM A36 (raw coal screen section) and can be significantly increased by applying HDI and LSE coating processes. Field testing has been performed using a LSE-treated screen panel and it showed a 2 times improvement of the service life.
Reversed phase chromatography of isoaccepting tRNA's from healthy and crown gall tissues from Nicotiana tabacum (1975)

RPC 5 (Reversed Phase Chromatography) of aminoacyl-tRNA's from healthy and crown gall (induced by Agrobacterium tume-faciens strain B6) tobacco tissues were compared for eleven amino acids. For ten amino acids: alanine, arginine, glutamic acid, glycine, isoleucine, leucine, lysine, methionine, tyrosine, and valine, no qualitative or quantitative differences could be detected between aminoacyl-tRNA's from both sources. Phenylalanyl-tRNA's from crown gall tissues gave two peaks on RPC 5; the minor early eluting species (peak 1) was always absent in elution profiles of phenylalanyl-tRNA's from healthy tissues or from tobacco leaves. After the "Y" base was removed by pH 2.9 treatment, peak 2 of phenylalanine tRNA was shifted to the position of peak 1.
Organosilane Effects on Organotitanium-Catalyzed Styrene Polymerization (2007)

Organosilane reagents are introduced into organotitanium-mediated styrene polymerizations to produce atactic polystyrene with high activities (up to 106 g polymer/(mol Ti·h)) and narrow polydispersities. Previously recognized CGCTiMe2 systems having marginal styrene homopolymerization activity are shown to be up to 3 orders of magnitude more active for styrene homopolymerization upon addition of organosilane.
Open-ended system for high-level synthesis of flexible signal processors (1990)

CATHEDRAL-2nd is a new synthesis environment, intended to be the follow-up of the authors' current CATHEDRAL-II approach. It is tuned towards the mapping of complex medium sample rate DSP applications onto flexible microcoded architectures. An important novel feature, is the increase in architectural freedom. As opposed to the formerly predefined limited set of execution units (EXUs) in CATHEDRAL-II, the system supports more flexibility in the composition of EXUs. The paper treats the impact of this feature on scheduling, hardware assignment and memory management. >
A curated collection of human vaccination response signatures (2022)

Recent advances in high-throughput experiments and systems biology approaches have resulted in hundreds of publications identifying "immune signatures". Unfortunately, these are often described within text, figures, or tables in a format not amenable to computational processing, thus severely hampering our ability to fully exploit this information. Here we present a data model to represent immune signatures, along with the Human Immunology Project Consortium (HIPC) Dashboard ( www.hipc-dashboard.org ), a web-enabled application to facilitate signature access and querying. The data model captures the biological response components (e.g., genes, proteins, cell types or metabolites) and metadata describing the context under which the signature was identified using standardized terms from established resources (e.g., HGNC, Protein Ontology, Cell Ontology). We have manually curated a collection of &gt;600 immune signatures from &gt;60 published studies profiling human vaccination responses for the current release. The system will aid in building a broader understanding of the human immune response to stimuli by enabling researchers to easily access and interrogate published immune signatures.
Free-Space Optical Gateway Placement in Hybrid Wireless Mesh Networks (2009)

<para xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> The capacity of wireless mesh networks (WMN) must usually be upgraded as usage demands evolve over time. This is normally done by adding gateways which serve to increase the backhaul capacity of the network. In this paper we consider adding capacity in this manner using free-space optical (FSO) backhaul links. To accomplish this, we formulate a joint clustering and gateway placement problem which includes the strong rate-distance dependence of practical FSO links. The formulation incorporates the positions of existing wireline gateways and minimizes the number of additional hybrid-FSO/RF gateways which are needed to satisfy the target capacity requirements. After showing the complexity of the problem, a solution that is motivated by genetic algorithms is proposed. The performance of our algorithm is then compared to an optimal solution generated via an integer linear program (ILP) for small WMNs. The proposed algorithm is then modified to allow for balancing the traffic load that is carried by each gateway in the WMN. Many scenarios are considered which demonstrate the value of using FSO backhaul links to obtain post-deployment capacity upgrades in response to changes in user traffic. </para>
Synthesis of protoheme IX derivatives with a covalently linked proximal base and their human serum albumin hybrids as artificial hemoprotein (2004)

The simple one-pot reaction of protoporphyrin IX and omega-(N-imidazolyl)alkylamine or O-methyl-L-histidyl-glycine with benzotriazol-1-yl-oxytris(dimethylamino)phosphonium hexafluorophosphate at room temperature produced a series of protoporphyrin IX species with a covalently linked proximal base at the propionate side-chain. The central iron was inserted by the general FeCl2 method, converting the free-base porphyrins to the corresponding protoheme IX derivatives. Mesoporphyrin IX and diacetyldeuteroporphyrin IX analogues were also prepared by the same procedure. The Fe(II) complexes formed dioxygen (O2) adducts in dimethylformamide at 25 degrees C. Some of them were incorporated into the hydrophobic domain of recombinant human serum albumin (rHSA), providing albumin-heme hybrids (rHSA-heme), which can bind and release O2 in aqueous media (pH 7.3, 25 degrees C). The oxidation process of converting the dioxygenated heme in rHSA to the inactive Fe(III) state obeyed first-order kinetics, indicating that the mu-oxo dimer formation was prevented by the immobilization of heme in the albumin scaffold. The rHSA-heme, in which the histidylglycil tail coordinates to the Fe(II) center, showed the most stable O2 adduct complexes.
Characterizing Tumor Response to Chemotherapy at Various Length Scales Using Temporal Diffusion Spectroscopy (2012)

Measurements of apparent diffusion coefficient (ADC) using magnetic resonance imaging (MRI) have been suggested as potential imaging biomarkers for monitoring tumor response to treatment. However, conventional pulsed-gradient spin echo (PGSE) methods incorporate relatively long diffusion times, and are usually sensitive to changes in cell density and necrosis. Diffusion temporal spectroscopy using the oscillating gradient spin echo (OGSE) sequence is capable of probing short length scales, and may detect significant intracellular microstructural changes independent of gross cell density changes following anti-cancer treatment. To test this hypothesis, SW620 xenografts were treated by barasertib (AZD1152), a selective inhibitor of Aurora B kinase which causes SW620 cancer cells to develop polyploidy and increase in size following treatment, ultimately leading to cell death through apoptosis. Following treatment, the ADC values obtained by both the PGSE and low frequency OGSE methods increased. However, the ADC values at high gradient frequency (i.e. short diffusion times) were significantly lower in treated tumors, consistent with increased intracellular restrictions/hindrances. This suggests that ADC values at long diffusion times are dominated by tumor microstructure at long length scales, and may not convey unambiguous information of subcellular space. While the diffusion temporal spectroscopy provides more comprehensive means to probe tumor microstructure at various length scales. This work is the first study to probe intracellular microstructural variations due to polyploidy following treatment using diffusion MRI in vivo. It is also the first observation of post-treatment ADC changes occurring in opposite directions at short and long diffusion times. The current study suggests that temporal diffusion spectroscopy potentially provides pharmacodynamic biomarkers of tumor early response which distinguish microstructural variations following treatment at both the subcellular and supracellular length scales.
Infection of Human Gastrointestinal Cells by HIV-1 (1990)

Human immunodeficiency virus (HIV-1) infected and replicated in primary cultures of normal human ileal and colonic epithelial cells. Monocyte-tropic strains (ADA, 24, and 36) were better able to replicate in the gastrointestinal (GI) cells than the T-cell-tropic HIV strain HTLV-IIIB. In some cultures, virus replication persisted through several months. Intestinal epithelium may be an initial target and reservoir for HIV and a vector for virus dissemination and transmission.
The Synthesis and 16S A‐Site rRNA Recognition of Carbohydrate‐Free Aminoglycosides. (2006)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access the actual ChemInform Abstract, please click on HTML or PDF.
Correlates of adjuvanticity: A review on adjuvants in licensed vaccines (2018)

After decades of slow progress, the last years have seen a rapid acceleration of the development of adjuvanted vaccines which have lately been approved for human use. These adjuvants consist of different components, e.g. aluminium salts, emulsions such as MF59 and AS03, Toll-like receptor (TLR) agonists (CpG ormonophosphoryl lipid A (MPL) adsorbed on aluminium salts as in AS04) or combination of immunopotentiators (QS-21 and MPL in AS01). Despite their distinctive features, most of these adjuvants share some key characteristics. For example, they induce early activation (although at different levels) of innate immunity which then translates into higher antibody and cellular responses to the vaccine antigens. In addition, most of these adjuvants (e.g. MF59, AS03, AS04) clearly induce a wider breadth of adaptive responses able to confer protection against, for example, heterovariants of the influenza viruses (MF59, AS03) or against human papillomavirus strains not contained in the vaccine (AS04). Finally, the use of some of these adjuvants has contributed to significantly enhance the immune response and the efficacy and effectiveness of vaccines in the elderly who experience a waning of the immune responsiveness to infection and vaccination, as shown for MF59- or AS03-adjuvanted influenza vaccines and AS01-adjuvanted herpes zoster vaccine. These results, together with the track record of acceptable safety profiles of the adjuvanted vaccines, pave the way for the development of novel vaccines at the extremes of age and against infections with a high toll of morbidity and mortality. Here, we review the mechanisms associated with the performance of those adjuvanted vaccines in animal models and in humans through recent advances in systems vaccinology and biomarker discovery. We also provide some perspectives on remaining knowledge gaps but also on opportunities that could accelerate the development of new vaccines.
PEPTIDE ANTAGONISTS OF LH-RH: LARGE INCREASES IN ANTIOVULATORY ACTIVITIES PRODUCED BY BASIC D-AMINO ACIDS IN THE SIX POSITION (1982)

It has been assumed, usually with good reason, that D-amino acids with large aromatic side-chains must be present in position 6 of both LH-RH superagonists and antagonists for the highest levels of biological activity to be reached. However, using one of a recent generation of potent lH-RH inhibitory analogs as a model, we have found that the insertion of D-lysine or, better still, D-arginine in this position results in greater antiovulatory activity in the rat over corresponding D-phenylalanine6- and D-tryptophan6-analogs. For instance, [Ac-D-p-Cl-Phe1,2,D-Trp3,D-Arg6,D-Ala10]-LH-RH exhibits antiovulatory activity at a dose of 750 ng per animal and appears to be the first competitive antagonist with activity in the nanogram region in vivo. This effect seems to be highly dependent on the degree of basicity of the amino acid side-chain since D-amino acids with neutral or acidic groups produced far less active compounds.
Delivery of GalNAc-Conjugated Splice-Switching ASOs to Non-hepatic Cells through Ectopic Expression of Asialoglycoprotein Receptor (2019)

Splice-switching antisense oligonucleotides (ASOs) are promising therapeutic tools to target various genetic diseases, including cancer. However, in vivo delivery of ASOs to orthotopic tumors in cancer mouse models or to certain target tissues remains challenging. A viable solution already in use is receptor-mediated uptake of ASOs via tissue-specific receptors. For example, the asialoglycoprotein receptor (ASGP-R) is exclusively expressed in hepatocytes. Triantennary N-acetylgalactosamine (GalNAc) (GN3)-conjugated ASOs bind to the receptor and are efficiently internalized by endocytosis, enhancing ASO potency in the liver. Here we explore the use of GalNAc-mediated targeting to deliver therapeutic splice-switching ASOs to cancer cells that ectopically express ASGP-R, both in vitro and in tumor mouse models. We found that ectopic expression of the major isoform ASGP-R1 H1a is sufficient to promote uptake and increase GN3-ASO potency to various degrees in four of five tested cancer cells. We show that cell-type-specific glycosylation of the receptor does not affect its activity. In vivo, GN3-conjugated ASOs specifically target subcutaneous xenograft tumors that ectopically express ASGP-R1, and modulate splicing significantly more strongly than unconjugated ASOs. Our work shows that GN3-targeting is a useful tool for proof-of-principle studies in orthotopic cancer models, until endogenous receptors are identified and exploited for efficiently targeting cancer cells.
Supplementary Data from Overexpression of Class III β-Tubulin Predicts Good Response to Taxane-Based Chemotherapy in Ovarian Clear Cell Adenocarcinoma (2023)

Supplementary Data from Overexpression of Class III β-Tubulin Predicts Good Response to Taxane-Based Chemotherapy in Ovarian Clear Cell Adenocarcinoma
Fabrication of Stretchable Organic–Inorganic Hybrid Thin-Film Transistors on Polyimide Stiff-Island Structures (2015)

In this study, stretchable organic-inorganic hybrid thin-film transistors (TFTs) are fabricated on a polyimide (PI) stiff-island/elastomer substrate using blends of poly(vinylidene fluoride-trifluoroethylene) [P(VDF-TrFE)] and poly(methyl methacrylate) (PMMA) and oxide semiconductor In-Ga-Zn-O as the gate dielectric and semiconducting layer, respectively. Carrier mobility, Ion/Ioff ratio, and subthreshold swing (SS) values of 6.1 cm2 V(-1) s(-1), 10(7), and 0.2 V/decade, respectively, were achieved. For the hybrid TFTs, the endurable maximum strain without degradation of electrical properties was approximately 49%. These results correspond to those obtained in the first study on fabrication of stretchable hybrid-type TFTs on elastomer substrate using an organic gate insulator and oxide semiconducting active channel structure, thus indicating the feasibility of a promising device for stretchable electronic systems.
Defective adenoviruses as virus vectors for veterinary vaccines. (1995)

International audience
Relationship of LVEF and Myocardial Scar to Long-Term Mortality Risk and Mode of Death in Patients With Nonischemic Cardiomyopathy (2021)

Myocardial scar and LVEF are both risk markers for all-cause and cardiac death in patients with nonischemic cardiomyopathy. However, whereas myocardial scar has strong and incremental prognostic value for SCD risk stratification, LVEF has no incremental prognostic value over clinical measures. Scar assessment should be incorporated into patient selection criteria for primary prevention implantable cardioverter-defibrillator placement.
NEW TENDENCIES AND CONSEQUENCES REGARDING OCCUPATIONAL PATHOLOGY IN ARAD COUNTY (2011)

7 SC Medicina Muncii-Dr. Morariu SRL ABSTRACT. Phytoncides and phytoalexins are antibiotic substances that have been isolated from a large number of plants .Tropical plants in particular possess many antibacterial compounds, such as sophoraflavanone G (Sophora), calozeylaxanthone (Calophyllum), α-mangostin, and the stilbene olygomers of gnemonal B and gnetin E. Other plants like garlic (Allium sativum) and onion (Allium cepa), mustard (Sinapis alba), horseradish (Armoracia rusticana), radish (Raphanus sativus) and lichens (like Centraria islandica and Usnea barbata) also manifest antibiotic properties. The antibacterial activities of sophoraflavanone G.