gebhart/patrepeval huggingface dataset:
Siglec-9 Restrains Antibody-Dependent Natural Killer Cell Cytotoxicity against SARS-CoV-2 (2023)

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection alters the immunological profiles of natural killer (NK) cells. However, whether NK antiviral functions are impaired during severe coronavirus disease 2019 (COVID-19) and what host factors modulate these functions remain unclear. We found that NK cells from hospitalized COVID-19 patients degranulate less against SARS-CoV-2 antigen-expressing cells (in direct cytolytic and antibody-dependent cell cytotoxicity [ADCC] assays) than NK cells from mild COVID-19 patients or negative controls. The lower NK degranulation was associated with higher plasma levels of SARS-CoV-2 nucleocapsid antigen. Phenotypic and functional analyses showed that NK cells expressing the glyco-immune checkpoint Siglec-9 elicited higher ADCC than Siglec-9<sup>-</sup> NK cells. Consistently, Siglec-9<sup>+</sup> NK cells exhibit an activated and mature phenotype with higher expression of CD16 (FcγRIII; mediator of ADCC), CD57 (maturation marker), and NKG2C (activating receptor), along with lower expression of the inhibitory receptor NKG2A, than Siglec-9<sup>-</sup> CD56<sup>dim</sup> NK cells. These data are consistent with the concept that the NK cell subpopulation expressing Siglec-9 is highly activated and cytotoxic. However, the Siglec-9 molecule itself is an inhibitory receptor that restrains NK cytotoxicity during cancer and other viral infections. Indeed, blocking Siglec-9 significantly enhanced the ADCC-mediated NK degranulation and lysis of SARS-CoV-2-antigen-positive target cells. These data support a model in which the Siglec-9<sup>+</sup> CD56<sup>dim</sup> NK subpopulation is cytotoxic even while it is restrained by the inhibitory effects of Siglec-9. Alleviating the Siglec-9-mediated restriction on NK cytotoxicity may further improve NK immune surveillance and presents an opportunity to develop novel immunotherapeutic tools against SARS-CoV-2 infected cells. <b>IMPORTANCE</b> One mechanism that cancer cells use to evade natural killer cell immune surveillance is by expressing high levels of sialoglycans, which bind to Siglec-9, a glyco-immune checkpoint molecule on NK cells. This binding inhibits NK cell cytotoxicity. Several viruses, such as hepatitis B virus (HBV) and HIV, also use a similar mechanism to evade NK surveillance. We found that NK cells from SARS-CoV-2-hospitalized patients are less able to function against cells expressing SARS-CoV-2 Spike protein than NK cells from SARS-CoV-2 mild patients or uninfected controls. We also found that the cytotoxicity of the Siglec-9<sup>+</sup> NK subpopulation is indeed restrained by the inhibitory nature of the Siglec-9 molecule and that blocking Siglec-9 can enhance the ability of NK cells to target cells expressing SARS-CoV-2 antigens. Our results suggest that a targetable glyco-immune checkpoint mechanism, Siglec-9/sialoglycan interaction, may contribute to the ability of SARS-CoV-2 to evade NK immune surveillance.
Quantifying CD4 receptor protein in two human CD4+ lymphocyte preparations for quantitative flow cytometry (2014)

Because of the lyophilization process applied to Cyto-Trol control cells, a lower CD4 density value, defined as the copy number of CD4 receptors per CD4+ lymphocyte, averaged over three different production lots is most likely explained by the loss of the CD4 receptors on damaged and/or broken microvilli where CD4 receptors reside. Steric hindrance of antibody binding and the association of CD4 receptors with other biomolecules likely contribute significantly to the nearly 50% lower CD4 receptor density value for cryopreserved PBMC determined from flow cytometry compared to the value obtained from MRM MS.
Supplementary Table 4 from <i>ETV6</i> Deficiency Unlocks ERG-Dependent Microsatellite Enhancers to Drive Aberrant Gene Activation in B-Lymphoblastic Leukemia (2023)

&lt;p&gt;Supplementary table 4 lists ETV6-regulated gene-enhancer linkages&lt;/p&gt;
Experiments in fast restoration using GMPLS in optical/electronic mesh networks (2002)

We present experimental results demonstrating that generalised multi-protocol label switching (GMPLS) with resource reservation protocol (RSVP) can be applied to optical/electrical mesh networks to yield ultra-fast provisioning and restoration times competitive with SONET rings, confirming results previously predicted by simulation.
Effects of Turbocharger Rotation Inertia on Instantaneous Turbine Efficiency in a Turbocharged-Gasoline Direct Injection (T-GDI) Engine (2020)

Abstract The effects of turbocharger rotation inertia on instantaneous turbine efficiency were analyzed in a 2.0 L four-cylinder twin-scroll turbocharged gasoline direct injection (T-GDI) engine. Two turbochargers of different weights were prepared for comparison purposes: base T/C and light T/C. In order to evaluate the instantaneous efficiency of each turbocharger, a combination of engine tests and a 1D simulation was conducted. In the experiments, the instantaneous exhaust gas pressures, upstream and downstream of the turbine, were measured. The instantaneous turbocharger rotation speed was also measured. The instantaneous temperature and mass flow rate of the exhaust gas were taken from the 1D simulation results. Through a combination of measuring and simulating exhaust gas states for the turbines, the instantaneous turbine blade speed ratio (BSR), total-to-static turbine efficiency (ηts), reduced mass flow rate (φ), and reduced turbine speed (Nred) were calculated. The light T/C showed higher fluctuations in the instantaneous turbine rotation speed compared to the base T/C. This greater response from the light T/C is due to experiencing less inertia loss, this leads to higher fluctuations in the instantaneous Nred, BSR, ηts, and φ. As a result, the light T/C shows higher turbine efficiency at certain points after the start of the exhaust gas pulse cycle. This is because the greater response of the light T/C lead the operating conditions of the turbine to more efficient regions in the turbine map.
Abstract 6247: Drug delivery with real time customizable micro-scaled continuous liquid interface printed 3D devices (2020)

Abstract Background: Surgery is a key treatment for cancer. However, local recurrence after surgery is common and can lead to significant morbidity and mortality. Current strategies to prevent recurrence include intraoperative radiation or systemic chemotherapy. We hypothesize that local chemotherapy delivery can prevent recurrence while reducing toxicity. To accomplish this, we propose to engineer 3D printed devices that can deliver chemotherapy in a localized and prolonged fashion. Methods: Paclitaxel and a cisplatin prodrug were dissolved into a liquid resin mixture of PEG550-DMA and HEMA. Custom 3D patches were printed on a Carbon 3D continuous liquid interface printer with microneedles protruding from one side of a flat surface substrate. A non-drug loaded flat backing was also printed from the same resin, and adhered to back side of the microneedle patch. Tumor growth recurrence inhibition post surgical resection was assessed in NU/NU mice in both 344SQ/Luc and A431 tumor models. Tumors were inoculated subcutaneously (106 344SQ/Luc, 5 × 105 A431) and allowed to grow until they reached 5-7mm in length. Tumors were then surgically resected and mice were either given an IV drug treatment, or had a 3D printed patch implanted in the surgical pocket. Tumor recurrence was evaluated with both luciferase bioluminescence imaging and physical measurement of tumor size. Toxicity parameters were analyzed with enzyme and CBC tests. Results: All drug loaded patches showed significant tumor growth inhibition compared to their corresponding IV drug treatment groups. While single drug loaded patches were significant in their inhibition of tumor growth compared to IV delivery of the same drug, dual drug loaded patches showed the most significant tumor inhibition compared to either single IV drug treatment or a dual IV drug treatment. There were no significant differences in measured toxicity parameters between groups. Conclusions: Our data demonstrated that local drug delivery through a customizable 3D continuous liquid interface printed drug loaded patch can improve tumor growth recurrence inhibition post surgical resection. Citation Format: C. Tilden Hagan, Cameron J. Bloomquist, Joseph M. DeSimone, Andrew Z. Wang. Drug delivery with real time customizable micro-scaled continuous liquid interface printed 3D devices [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 6247.
Viability and longevity of pollen from transgenic and nontransgenic tall fescue (Festuca arundinacea) (Poaceae) plants (2004)

Pollen is an important vector of gene flow in plants, particularly for outcrossing species like tall fescue. Several aspects of pollination biology were investigated using pollen from transgenic and nontransgenic plants of tall fescue (Festuca arundinacea Schreb.), the most important forage species worldwide of the Festuca genus. To effectively assess in vitro pollen viability in tall fescue, an optimized germination medium (0.8 mol/L sucrose, 1.28 mmol/L boric acid and 1.27 mmol/L calcium nitrate) was developed. Treatment with relatively high temperatures (36° and 40°C) and high doses of UV-B irradiation (900-1500 μW/cm(2)) reduced pollen viability, while relative humidity did not significantly influence pollen viability. Viability of pollen from transgenic progenies (T1 and T2) was similar to that from seed-derived control plants. Pollen from primary transgenics (T0) and primary regenerants (R0) had various levels of viability. Hand pollination using the primary regenerants and transgenics revealed that no seed set could be obtained when pollen viability was lower than 5%. Pollen from transgenic progenies and nontransgenic control plants could survive up to 22 h under controlled conditions in growth chamber. However, under sunny atmospheric conditions, viability of transgenic and nontransgenic pollen reduced to 5% in 30 min, with a complete loss of viability in 90 min. Under cloudy atmospheric conditions, pollen remained viable up to 240 min, with about 5% viability after 150 min. This report is the first on pollen viability and longevity in transgenic forage grasses and could be useful for risk assessment of transgenic plants.
Performance Testing for Dry Powder Inhaler Products: Towards Clinical Relevance (2022)

It is well established that the critical performance metrics for aerosol products are aerodynamic particle size distribution (APSD) and delivered dose uniformity (DDU). In broad terms, these performance characteristics dictate the efficiency and reproducibility with which an aerosol is administered clinically. However, these properties alone do not support in-vitro, in-vivo correlations. There have been numerous publications attempting to more directly link product performance testing to physiological relevance or further to draw direct correlations of relevance to bioequivalence testing for the development of generic products. While these novel methods have been employed in product development activity, their suitability for compendial testing has yet to be established. This paper explores the potential to establish biologically relevant compendial standards for dry powder inhaler products while maintaining accuracy and reproducibility of data collected to support the quality and performance of the product.
A Kinematic Modeling Framework for Prediction of Instantaneous Status of Towing Vehicle Systems (2018)

&lt;div&gt;A kinematic modeling framework was established to predict status (position, displacement, velocity, acceleration, and shape) of a towing vehicle system with different driver inputs. This framework consists of three components: (1) a state space model to decide position and velocity for the vehicle system based on Newton’s second law; (2) an angular acceleration transferring model, which leads to a hypothesis that the each towed unit follows the same path as the towing vehicle; and (3) a polygon model to draw instantaneous polygons to envelop the entire system at any time point. Input parameters of this model include initial conditions of the system, real-time locations of a reference point (e.g. front center of the towing vehicle) that can be determined from a beacon and radar system, and instantaneous accelerations of this system, which come from driver maneuvers (accelerating, braking, steering, etc.) can be read from a data acquisition system installed on the towing vehicle. The output of the model is instantaneous polygons that render approximate outline of the towing vehicle system at any time point. The instantaneous polygons will be converted to control areas for system control. This model was validated for predicting instantaneous shapes of the system with one towing vehicle and four towed units. This model can be easily modified and extended to be used for other tractor-trailer systems or even road trains.&lt;/div&gt;
Effects of heating on hydrophobicity, viscosity, and gelling properties of soy products (2005)

The co-product of soybean after oil extraction is the meal, which is rich in protein. From this meal, protein concentrate and protein isolate are prepared and are commercially available as functional ingredients. Thermal treatment is the most common step applied to foods during processing. Changes in structural and functional properties can be affected by thermal or chemical treatments. The objective of this study was to evaluate the effect of heat on surface hydrophobicity, gelling properties, and viscosity of soy meal (SM), soy protein concentrate (SPC), and soy protein isolate (SPI). The soy products were subjected to heat at varying temperatures and heating times. Viscosity of soy protein products treated with heat increased for SM when temperature and heating times increased, but decreased for SPC and SPI. This may be due to the polysaccharides present in SM that could form starch gelation and increase meal viscosity. The surface hydrophobicity of the soy products increased when the proteins were treated with heat, possibly due to heat exposing the hydrophobic amino acids buried within the protein molecule making them become more hydrophobic on the surface of the molecule. When 8% suspensions (protein basis) were heated at 100°C, all soy products formed firm gels, indicating that protein plays an important role in gel network formation. Precaution must be taken to maintain functionality when heat processing is applied to food systems that contain soy protein products as functional ingredients.
The sensitivity to honey of Gram-positive cocci of clinical significance isolated from wounds (2002)

A possible role for honey in the treatment of wounds colonized by antibiotic-resistant bacteria is indicated.
Immunochemotherapy With Rituximab and Overall Survival in Patients With Indolent or Mantle Cell Lymphoma: A Systematic Review and Meta-analysis (2007)

In patients with indolent or mantle cell lymphoma, R-chemo is superior to chemotherapy alone with respect to overall survival.
Sine-Cosine Techniques for Detecting Fast Optical Frequency Variations With Ultra-High Resolution (2022)

We introduce the concept of sine-cosine optical frequency detection (OFD) for measuring fast optical frequency variations with the ultra-high spectral resolution for applications requiring rapidly tunable or low frequency-jitter lasers, including coherent detection based distributed sensing and communication systems and tunable diode laser spectroscopy (TDLS) systems, for which conventional spectral measurement techniques cannot meet the speed and spectral resolution requirements. The concept relies on representing the frequency-dependent differential phase of the laser light passing through two different optical paths as sine and cosine functions to calculate the optical frequency, similar to the sine-cosine encoder concept commonly used for obtaining the rotation information of a motor, which can simultaneously achieve both high spectral resolution and wide frequency range without mutual constraints. We present multiple schemes, some are seemingly very different, for implementing the concept and demonstrate that all frequency-related parameters of a tunable light source, including frequency tuning rate, nonlinearity, ripple, range, duty cycle, repeatability, and power swing can be precisely characterized. In addition, the frequency jitter of a fixed narrow line-width laser can also be accurately measured. Ultra-fast optical frequency sweep with a rate in excess of 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">7</sup> nm/s and a resolution down to MHz is demonstrated with a differential delay of only 0.8 mm, suggesting that the concept is feasible to be implemented with photonic integrated circuit (PIC). Our work is attractive for applications such as coherent distributed sensing (OCT, OFDR and FMCW lidar), coherent detection, FBG sensing interrogation and TDLS, especially considering that some schemes can be integrated on PIC chips.
Mutations in accessory DNA replicating functions alter the relative mutation frequency of herpes simplex virus type 1 strains in cultured murine cells (1994)

The contribution of the herpes simplex virus type 1 (HSV-1)-encoded uracil DNA glycosylase (UNG), thymidine kinase (TK), and dUTPase to the relative mutant frequency (RMF) of the virus in cultured murine cells was examined. A panel of HSV-1 mutants that lacked singly or doubly the UNG, TK, or dUTPase activity were generated by disruption of the enzyme coding regions with the Escherichia coli beta-galactosidase (beta-gal) gene in strain 17syn+. To establish a baseline RMF of strain 17syn+, the beta-gal gene was inserted into the UL3 locus. In all of the viruses, the beta-gal insert served as a phenotypic marker of RMF. A mutant plaque was identified by the lack of beta-gal activity and, in selected cases, positive in situ hybridization for beta-gal sequences. Replication kinetics in NIH 3T3 cells demonstrated that all of the mutants replicated efficiently, generating stocks with equivalent titers. Two independently generated UL3-beta-gal viruses were examined and established a baseline RMF of approximately 0.5% in both NIH 3T3 and LM TK- cells. Loss of dUTPase activity resulted in viruses with fivefold-increased RMFs, indicating that the HSV-1 dUTPase has an antimutator function. The RMF observed for the tk- viruses was reduced as much as 40-fold (RMF of 0.02%), suggesting that the viral TK is a mutator activity. The RMF of two independent UNG- viruses showed no significant difference from the baseline RMF in limited passage; however, following successive passage, the data suggested that UNG activity serves as an antimutator. These results have implications for the natural history of HSV and the development of antiviral therapies.
Synthesis of Reactive Functionalized Oligo(p-phenylene sulfide)s (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of Reactive Functionalized Oligo(p-phenylene sulfide)sEishun Tsuchida, Kimihisa Yamamoto, Kenichi Oyaizu, Fumiaki Suzuki, Allan S. Hay, and Zhi Yuan WangCite this: Macromolecules 1995, 28, 2, 409–416Publication Date (Print):January 1, 1995Publication History Published online1 May 2002Published inissue 1 January 1995https://pubs.acs.org/doi/10.1021/ma00106a001https://doi.org/10.1021/ma00106a001research-articleACS PublicationsRequest reuse permissionsArticle Views493Altmetric-Citations35LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Self-Assembled Binary Superlattices of CdSe and Au Nanocrystals and Their Fluorescence Properties (2008)

Different types of Binary Nanoparticle Superlattices (BNSLs) have been self-assembled from monodisperse 8.7 nm CdSe and 5.5 nm Au nanocrystals. Fluorescence spectroscopy studies of AlB2-type BNSL of CdSe and Au nanocrystals revealed considerably decreased fluorescence and a shortened fluorescence lifetime of the CdSe NCs in BNSLs compared to the CdSe-only sample.
Cocycle twists of algebras (2016)

Let A=⊕n=0∞An be a connected graded k-algebra over an algebraically closed field k (thus A0 = k). Assume that a finite Abelian group G, of order coprime to the characteristic of k, acts on A by graded automorphisms. In conjunction with a suitable cocycle, this action can be used to twist the multiplication in A. We study this new structure and, in particular, we describe when properties like Artin–Schelter regularity are preserved by such a twist. We then apply these results to examples of Rogalski and Zhang.
Expression of genes involved in early cell fate decisions in human embryos and their regulation by growth factors (2008)

Little is understood about the regulation of gene expression in human preimplantation embryos. We set out to examine the expression in human preimplantation embryos of a number of genes known to be critical for early development of the murine embryo. The expression profile of these genes was analysed throughout preimplantation development and in response to growth factor (GF) stimulation. Developmental expression of a number of genes was similar to that seen in murine embryos (OCT3B/4, CDX2, NANOG). However, GATA6 is expressed throughout preimplantation development in the human. Embryos were cultured in IGF-I, leukaemia inhibitory factor (LIF) or heparin-binding EGF-like growth factor (HBEGF), all of which are known to stimulate the development of human embryos. Our data show that culture in HBEGF and LIF appears to facilitate human embryo expression of a number of genes: ERBB4 (LIF) and LIFR and DSC2 (HBEGF) while in the presence of HBEGF no blastocysts expressed EOMES and when cultured with LIF only two out of nine blastocysts expressed TBN. These data improve our knowledge of the similarities between human and murine embryos and the influence of GFs on human embryo gene expression. Results from this study will improve the understanding of cell fate decisions in early human embryos, which has important implications for both IVF treatment and the derivation of human embryonic stem cells.
Ein totalsynthetischer Globo-H-Kohlenhydrat-Impfstoff verursacht eine gezielte humorale Immunantwort bei Prostatakrebs-Patienten: der Beweis des Prinzips (1999)

Osteoblasten: ja, Blutplättchen: nein! Knochenimplantate müssen sich mit dem umgebenden Gewebe verbinden, um möglichst gut einwachsen zu können. Ein neues Verfahren wird beschrieben, das auf der kovalenten Anknüpfung eines hochselektiven RGD-Peptides an Polymethylmethacrylat (PMMA) basiert (siehe Bild). Osteoblasten binden sehr effektiv an so beschichtete Oberflächen und werden zur Proliferation stimuliert.