gebhart/patrepeval huggingface dataset:
Rapid and sensitive determination of cytokine release from cells without the need for sample transfer (2020)

Abstract We have utilized NanoLuc® Binary Technology (NanoBiT®) to develop a homogeneous and rapid assay method (≤ 70 min completion time) to measure cytokines released from cells in culture without the need for sample transfer and requiring only a standard, plate-reading luminometer for signal acquisition. Separate antibodies to a specific cytokine are labeled with the small, 11-amino acid subunit of NanoBiT luciferase (SmBiT) or its 17.6 kDa complementary subunit (LgBiT). When SmBiT- and LgBiT-labeled antibodies converge on the target cytokine, the resultant proximity of SmBiT and LgBiT reconstitutes a bright luciferase that produces light proportional to analyte levels. In this manner, immunoassays have been developed for several cytokines, including IL-1β, IL-2, IL-6 and IFN-γ. In general, these assays share LLODs &amp;lt; 10 pg/ml and linearity over three logs of analyte concentration, mitigating the need for sample dilution. Following 24 h treatment of human PBMCs in 96-well plates with vehicle, LPS, R848, or a combination of PMA and ionomycin, cytokine detection reagents were added directly to the culture wells containing cells and medium. Depending on stimulus, maximal signal to background ratios (S/B) achieved for the cytokines were 347-, 450-, 580- and 655-fold for IL-1β, IL-2, IL-6 and IFN-γ, respectively. In a cell model comprised of activated T cells and target Raji B cells induced for 20 h with increasing bispecific T-cell engager Blincyto®, release of IL-2 and IFN-γ were observed with an EC50 of ~0.2 ng/ml and maximal S/B for IL-2 and IFN-γ of 82- and 168-fold, respectively. The implementation of this novel detection chemistry will enable rapid “add-and-read” assays for cytokine detection for both low- and high-throughput applications.
Morphological Failure Mechanisms in Tensile Tests of Crosslinked Polyurethanes With Poorly Developed Domain Structure (2015)

Macroscopic failure of polyurethane materials of 30 wt.-% hard-segment content is related to microstructure evolution mechanisms. Topology and functionality (f = 2 … 4) of the polyols are varied. Samples are strained and small-angle X-ray scattering (SAXS) patterns are recorded. Only material PU-I (f = 2) passes the tensile test. Material PU-Hs – a H-shaped (f = 4) polyol with short arms – is not nanostructured. PU-Hl has long arms. It contains few hard domains placed at random. PU-Hl survives longer (strain: 1.8) than the other short-lived materials. Its isolated hard domains are not destroyed during straining. PU-X (f = 4, star-shaped) develops microfibrils: one-dimensional (1D) correlations among hard domains, as deduced from a chord distribution function (CDF) analysis. PU-I and PU-Y are based on 2- and 3-functional polyols. They contain many well-separated hard domains with one-dimensional (3D) connectivity. Their arrangement of hard domains evolves identically, but not the population density. In PU-I (and PU-X) hard domains fail during straining, in PU-Y the interdomain soft phase density decreases.
Stoichiometric and Catalytic Activation of the α- and β-2,3,4-Tri-O-Acetyl-5-Thioxylopyranosyl Bromide Inside the Cavity of the Pd3(dppm)3(CO)2+ Cluster (2003)

The title cluster (Pd(3)(2+)) exhibits a pronounced affinity for Br(-) ions to form the very stable Pd(3)(Br)(+) adduct. Upon a 2-electron reduction, a dissociative process occurs generating Pd(3)(0) and eliminating Br(-) according to an ECE mechanism (electrochemical, chemical, electrochemical). At a lower temperature (i.e. -20 degrees C), both ECE and EEC processes operate. This cluster also activates the C-Br bond, and this work deals with the reactivity of Pd(3)(2+) with 2,3,4-tri-O-acetyl-5-thioxylopyranosyl bromide (Xyl-Br), both alpha- and beta-isomers. The observed inorganic product is Pd(3)(Br)(+) again, and it is formed according to an associative mechanism involving Pd(3)(2+).Xyl-Br host-guest assemblies. In an attempt to render the C-Br bond activation catalytic, these species are investigated under reduction conditions at two potentials (-0.9 and -1.25 V vs SCE). In the former case, the major product is Xyl-H, issued from a radical intermediate Xyl(*) abstracting an H atom from the solvent. Evidence for Xyl(*) is provided by the trapping with TEMPO (2,2,6,6-tetramethyl-1-piperidinyloxy) and DMPO (5,5'-dimethylpyrroline-N-oxyde). In the second case, only one product is observed, 3,4-di-O-acetyl-5-thioxylal, which is issued from the Xyl(-)() intermediate anion.
Table S1 from A Four-Chemokine Signature Is Associated with a T-cell–Inflamed Phenotype in Primary and Metastatic Pancreatic Cancer (2023)

&lt;p&gt;Supplementary Table 1: Top 99 genes associating with CD8+ TIL counts in primary resected cases&lt;/p&gt;
Total Hip Arthroplasties in Patients Younger Than 45 Years (1990)

Eighty-one cemented total hip arthroplasties in patients younger than 45 years were reviewed with an average 9.2-year follow-up interval. The results were compared with the same group previously reported at an average follow-up time of 4.5 years. Clinically satisfactory results were 58% compared with 78% at 4.5 years. The revision rate almost tripled to 33%. Impending failure was present in 56% of those hips not yet revised. In the first study, technique was a primary determinant of success. In this report there was no influence of technique on the incidence of revision. Age did not influence revision rates either, with 35% in patients younger than 30 years and 32% in patients aged 30–45 years. Patients younger than 30 years had fewer satisfactory clinical results and a higher rate of impending failure. As in the first study, the best results were obtained in patients with inflammatory collagen disease aged from 30 to 45 years.
209 ROLE OF NITRIC OXIDE IN THE PATHOGENESIS OF A PREECLAMPSIA-LIKE SYNDROME IN RATS: (2005)

<h3></h3> We have developed a model of preeclampsia in the rat by administering desoxycorticosterone acetate (DOCA) and replacing their drinking water with 0.9% saline. The preeclamptic group (PDS group) showed several phenotypic characteristics of human preeclampsia, including increased blood pressure (BP), intrauterine growth restriction (IUGR), and proteinuria, when compared to the normal pregnant (NP) control group. To better understand this syndrome, we measured the nitric oxide (NO) in blood. NO has been reported to be implicated in the pathogenesis of preeclampsia. We also studied two other pregnant animal groups, one receiving only saline but no DOCA (PS) and the second receiving only DOCA with normal drinking water (PD). The results are as follows (data are means ± SD): (Table) We conclude that DOCA is largely responsible for the IUGR but the complete preeclamptic syndrome will not develop without saline. A possible explanation would be that saline is producing an initial status of volume expansion with increased cardiac output. The latter may trigger exaggerated renal hypertrophy, activation of the RAA, as well as release of growth and vasoconstrictive (and natriuretic) factors. Similar observations have been made in the preeclamptic patient.
Polymer characterization and gas permeability of poly(1-trimethylsilyl-1-propyne) [PTMSP], poly(1-phenyl-1-propyne) [PPP], and PTMSP/PPP blends (1996)

Pure gas and hydrocarbon vapor transport properties of blends of two glassy, polyacetylene-based polymers, poly(1-trimethylsilyl-1-propyne) [PTMSP] and poly(1-phenyl-1-propyne) [PPP], have been determined. Solid-state CP/MAS NMR proton rotating frame relaxation times were determined in the pure polymers and the blends. NMR studies show that PTMSP and PPP form strongly phase-separated blends. The permeabilities of the pure polymers and each blend were determined with hydrogen, nitrogen, oxygen, carbon dioxide, and n-butane. PTMSP exhibits unusual gas and vapor transport properties which result from its extremely high free volume. PTMSP is more permeable to large organic vapors, such as n-butane, than to small, permanent gases, such as hydrogen. PPP exhibits gas permeation characteristics of conventional low free volume glassy polymers; PPP is more permeable to hydrogen than to n-butane. In PTMSP/PPP blends, both n-butane permeability and n-butane/hydrogen selectivity increase as the PTMSP content of the blends increases. © 1996 John Wiley & Sons, Inc.
Multi-spectral SWIR lidar for imaging and spectral discrimination through partial obscurations (2020)

We have developed a multi-spectral SWIR lidar system capable of measuring simultaneous spatial-spectral information for imaging and spectral discrimination through partial obscurations. Our system utilizes a supercontinuum laser source and eight narrowband spectral channels in the 1000 nm to 1600 nm region. The system employs a steering mirror, which enables us to scan the region of interest and collect spectral and spatial data as a point-by-point scan. The system is designed to detect weak signal returns in the few-photon regime. The technique promises more capable classification and target detection of spectrally diverse targets in obscured environments with potential applications for mapping of ground type through forest canopy, pollution monitoring of water ways, and intelligence, surveillance, reconnaissance and target detection (ISRT). Custom targets designed to provide distinct spectral response are employed to ascertain the system’s response. The lidar system is calibrated by measuring the return signal from a highly reflective flat Spectralon target; this enables us to determine the reflectivity of the objects of interest. Spectral response of the targets are analyzed and their estimated reflectivity is reported. The same targets are studied in the presence of two partial obscurants. The objects are easily identified even though the return signal is attenuated by a factor of seven. The general spectral shape of the targets are preserved in the presence of the obscurants. More challenging objects and environments and various methods to recover the spectral response of the objects are currently being pursued.
ChemInform Abstract: Tri‐ and Tetra‐Peptides Incorporating an α‐Amino Acid at the Anomeric Position of Mannofuranose. (1995)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Nondestructive millimeter wave imaging and spectroscopy using dielectric focusing probes (2014)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation Jose A. Hejase, Steven S. Shane, Kyoung Y. Park, Premjeet Chahal; Nondestructive millimeter wave imaging and spectroscopy using dielectric focusing probes. AIP Conf. Proc. 18 February 2014; 1581 (1): 1588–1593. https://doi.org/10.1063/1.4865013 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAIP Publishing PortfolioAIP Conference Proceedings Search Advanced Search |Citation Search
The setwise stream classification problem (2014)

In many applications, classification labels may not be associated with a single instance of records, but may be associated with a data set of records. The class behavior may not be possible to infer effectively from a single record, but may be only be inferred by an aggregate set of records. Therefore, in this problem, the class label is associated with a set of instances both in the training and test data. Therefore, the problem may be understood to be that of classifying a set of data sets. Typically, the classification behavior may only be inferred from the overall patterns of data distribution, and very little information is embedded in any given record for classification purposes. We refer to this problem as the setwise classification problem.
New Architectures, protocols, and middleware for ad hoc collaborative computing (2003)

The existing Internet architecture and protocols are not suitable for the newly emerging ad hoc collaborative com-puting systems. New architectures, protocols, and middleware specifically designed for the new ad hoc collaborative computing systems are needed. While work is currently being done to adapt the Internet architecture and protocols so the new systems can be developed and deployed, the fundamental characteristics that make the Internet unsuitable for the new systems — address-based messaging, one-to-one communication, and central servers — still remain. We envision alternative architectures, protocols, and middleware with the opposite characteristics — no central servers, broadcast communication, and content-based messaging. Many-to-Many Invocation (M2MI) and the Many-to-Many Protocol (M2MP) are an initial realization of this vision. “No one pours new wine into old wineskins. If he does, the new wine will burst the skins, the wine will run out and the wineskins will be ruined. No, new wine must be poured into new wineskins. And no one after drinking old wine wants the new, for he says, ‘The old is better. ’ ” — Jesus of Nazareth (Luke 5:37–39) 1
Camp Beale live-site handheld-sensor data inversion and classification using advanced EMI models (2012)

ESTCP live-site UXO classification results are presented for cued data collected with two advanced EMI instruments, the cart-based 2 × 2 3D TEMTADS array and the Man Portable Vector (MPV) handheld sensor, at the former Camp Beale in California. There were two sets of targets of interest (TOI): the main set consisted of 105-mm, 81-mm, 60-mm, 37-mm and ISO projectiles, and the other (optional) set comprised site-specific fuzes and fuze fragments of varous sizes. The advanced models used for inversion and classification combine: 1) a joint-diagonalization (JD) algorithm that estimates the number of potential targets generating an anomaly directly from the measured data without need for inversion; 2) the ortho-normalized volume magnetic source (ONVMS) model, which locates targets, represents their EMI responses, and extracts their intrinsic feature vectors; and 3) a Gaussian mixture algorithm that uses extracted discrimination features to classify the corresponding buried objects as TOI or clutter. Initially the data are inverted using a combination of ONVMS and the differential evolution direct-search algorithm; this allows the determination of relevant intrinsic parameters, which in turn are classified by a mixture of clustering and library-matching techniques. This paper describes in more detail the main steps of the classification process and demonstrates the results obtained for the 2 × 2 3D TEMTADS and MPV data taken at Camp Beale, as scored independently by the Institute for Defense Analyses. The advanced models are seen to produce superb classification in both cases.
Protective immunity in mice vaccinated with the Schistosoma mansoni P-28-1 antigen. (1989)

The P28-1 Ag induces a strong protective immunity toward Schistosoma mansoni infection in various experimental models. T lymphocytes of mice immunized with the recombinant P28-1 Ag were stimulated in vitro by schistosome Ag of different development stages and by three P28-1 Ag-derived synthetic peptides. The most significant stimulation was achieved with the 24-43 peptide. The use of two fragments of this peptide showed that the P28-1 T lymphocyte specificity concerned essentially the NH2 terminal sequence of the 24-43 peptide. Moreover, T lymphocytes specific for the 24-43 peptide were stimulated by both schistosome Ag and the recombinant P28-1 protein. The passive transfer of (Th + Ts) lymphocytes recovered from P28-1 Ag-immunized mice increased the IgG response to P28-1 and its peptides during infection but did not protect against a challenge infection, such as the passive transfer of anti-P28-1 sera. In contrast, P28-1 specific Th cell lines maintained in culture for 2 mo, passively transferred a strong protection (50%) to infected mice. Supernatants of P28-1-specific T cells obtained after stimulation with the corresponding Ag, were able to confer cytotoxic properties to platelets and macrophages. The presence of IFN-gamma for the cytotoxicity mediated by platelets and macrophage activating factor for the cytotoxicity mediated by macrophages in these supernatants is in a large part responsible for the parasite killing observed. Finally, a preliminary immunogenetic approach with H-2 congenic mice on BALB background showed that the P28-1 Ag T cell response was under the control of the MHC and that the H-2b haplotype determined a low response to P28-1 Ag and its peptides while H-2d and k haplotypes determined high responders.
Periodic solitons in nonlocal nonlinear media (2007)

We identify periodic solitons in nonlocal nonlinear media: multi-hump soliton solutions propagating in a fully periodic fashion. We also demonstrate recurrences and breathers whose evolution is statistically periodic and discuss why some systems support periodic solitons while others do not.
Semiparametric methods for identification of tumor progression genes from microarray data (2004)

The use of microarray data has become quite commonplace in medical and scientific experiments. We focus here on microarray data generated from cancer studies. It is potentially important for the discovery of biomarkers to identify genes whose expression levels correlate with tumor progression. In this article, we develop statistical procedures for the identification of such genes, which we term tumor progression genes. Two methods are considered in this paper. The first is use of a proportional odds procedure, combined with false discovery rate estimation techniques to adjust for the multiple testing problem. The second method is based on order-restricted estimation procedures. The proposed methods are applied to data from a prostate cancer study. In addition, their finite-sample properties are compared using simulated data.
Theory and Experiment on an Elastically Moored Cylindrical Buoy (2006)

In this paper, we compare simulated forces and accelerations for a moored floating buoy with full-scale experimental results in real ocean waves. The buoy is moored with a wire connected by springs to a concrete foundation situated at the seafloor. This study aims to develop a computer model using potential theory with a linearized free-surface boundary condition to describe the motion of such a system. The intention is to use the model for future study of wave-energy absorption and design of converters. Another objective is to see how complex a model is required to get accurate results. The method used is computationally fast and makes it possible to couple linear buoy wave interaction with nonlinear generator models, so that different loads and latching can be studied. A computationally fast method is required to model farms of wave-energy converters
Bottom-emitting flip-chip VCSEL structure with reduced constraints on modulation response (2004)

Intrinsic and extrinsic limitations on conventional top-emitting high-speed VCSEL modulation bandwidth are discussed. A new VCSEL structure has been designed with reduced constraints on modulation bandwidth. The structure simultaneously addresses the primary extrinsic factors that limit the VCSEL modulation bandwidth. The new bottom-emitting, flip-chipped VCSEL structure improves heat-sinking, current injection uniformity, and reduces parasitic capacitance. We estimate the ultimate bandwidth of this new structure to be as high as 40Gb/s.
The Shadow of the Stasi (2019)

Many people outside Germany have heard of the Gestapo, and many outside Russia know what the KGB was, but few would know what is meant by the Stasi. If East Germans were clear about the existence of the MfS/ Stasi and clear that it was present from Rostock to Suhl and from Madgeburg to Frankfurt am Oder and widely represented in German Democratic Republic institutions, they were nevertheless shocked by revelations after November 1989 about the scale of its activities. East Germans expected there to be strict security in anything connected with defense, including defense industries. A terrible legacy of the Stasi were accusations against leading East German Wende politicians that their hands were not clean, that they had supped with the Stasi and had taken the 40 pieces of silver. The storming of the Stasi headquarters in the Normannenstraße of Berlin on 1990 and in other regional centers like Leipzig was a godsend for some Stasi officers and informers.
Faster rates of post-puberty kidney deterioration in males is correlated with elevated oxidative stress in males vs females at early puberty (2007)

Our data suggest that early puberty, with gender-related elevation in oxidative stress in males, is a key compromising factor on kidneys in males.
Ordered cyclic motifs contribute to dynamic stability in biological and engineered networks (2008)

Representation and analysis of complex biological and engineered systems as directed networks is useful for understanding their global structure/function organization. Enrichment of network motifs, which are over-represented subgraphs in real networks, can be used for topological analysis. Because counting network motifs is computationally expensive, only characterization of 3- to 5-node motifs has been previously reported. In this study we used a supercomputer to analyze cyclic motifs made of 3-20 nodes for 6 biological and 3 technological networks. Using tools from statistical physics, we developed a theoretical framework for characterizing the ensemble of cyclic motifs in real networks. We have identified a generic property of real complex networks, antiferromagnetic organization, which is characterized by minimal directional coherence of edges along cyclic subgraphs, such that consecutive links tend to have opposing direction. As a consequence, we find that the lack of directional coherence in cyclic motifs leads to depletion in feedback loops, where the number of nodes affected by feedback loops appears to be at a local minimum compared with surrogate shuffled networks. This topology provides more dynamic stability in large networks.
Visual Analytics for Power Grid Contingency Analysis (2014)

Contingency analysis employs different measures to model scenarios, analyze them, and then derive the best response to any threats. A proposed visual-analytics pipeline for power grid management can transform approximately 100 million contingency scenarios to a manageable size and form. Grid operators can examine individual scenarios and devise preventive or mitigation strategies in a timely manner. Power grid engineers have applied the pipeline to a Western Electricity Coordinating Council power grid model.