gebhart/patrepeval huggingface dataset:
Efficacy of function-enhanced, re-activatable, dual-specific CAR T cells pre-loaded with oncolytic virus for immunotherapy of high-grade glioma. (2023)

2062 Background: While cancer therapeutics have made tremendous progress within the past several decades, this benefit has not been seen in many primary cancers of the brain. Particularly confounding have been high-grade gliomas (HGG), which retain a dismal prognosis. Currently, novel therapies are being explored to rise to this unmet, critical need. One such therapy are CAR T cells, immune cells which have been engineered to target malignancy-specific antigens. Unfortunately, the efficacy of CAR T cell therapies against solid tumors is significantly limited, in large part due to impaired expansion/persistence in the immune suppressive tumor microenvironment (TME). Here we show that in vivo reactivation of CAR T cells through their native T Cell Receptor (TCR) by an oncolytic virus (OV) has therapeutic benefit in HGG. Methods: An EGFRvIII third-generation MSGV1 retroviral CAR construct containing the CD28, 4-1BB, and CD3z moieties, in tandem with the scFv derived from the human monoclonal antibody 139 and the marker Thy1.1 (38) was used to generate our CAR T cells. C57Bl/6 mice were used for in vivo experiments and both B16- and CT2A-EGFRvIII murine glioma cell lines were injected in the brain to model HGG. OVs and CAR T cells were given systemically by tail vein. OVs used include reovirus, vesicular stomatitis virus, and adenovirus. Results: By using OV in combination with EGFRvIII CAR T cells, we were able to generate a CD8 CAR population with TCR specificity for both the EGFRvIII and OV epitopes. These dual-specific (DS) CAR T expressed a memory phenotype and persisted for much longer than conventional CAR T cells. Further, we showed that these DS CAR T cells are more cytotoxic and can respond more rapidly than their conventional counterparts. We created a novel delivery mechanism for this combination OV + CAR T therapy using virus-loaded CAR T cells to bypass initial antiviral clearance from the immune system. Treatment with these OV-loaded CAR T cells lead to significant benefit in mice with HGG tumors which could be further enhanced by a systemic boost with OV, which rapidly re-activated DS CAR T cells against tumor and resulted in long-term cures of greater than 80% of treated animals. Conclusions: These promising results show that DS CAR T cells can overcome the critical therapeutic challenge of CAR T as a treatment for solid tumors. Given these promising results, we will go on to develop a clinical trial in which CAR T cells will be pre-loaded with OV and administered intravenously to patients with HGG, followed by systemic boosting with virus to re-activate DS CAR T cells against their tumor.
NaHCO 3 Dilates Mouse Afferent Arteriole Via Na + /HCO 3 − Cotransporters NBCs (2019)

Sodium bicarbonate has long been used to treat chronic kidney disease. It has been demonstrated to slow the decline in glomerular filtration rate in chronic kidney disease patient; however, the mechanisms are not completely understood. We hypothesized that NaHCO<sub>3</sub> dilates afferent arterioles (Af-Art) by stimulating nitric oxide (NO) release mediated by the Na<sup>+</sup>/HCO<sub>3</sub><sup>-</sup> cotransporter (NBC) contributing to the elevation in glomerular filtration rate. Isolated microperfused mouse renal Af-Art, preconstricted with norepinephrine (1 µmol/L), dilated 45±2% (n=6, <i>P</i><0.05) in response to NaHCO<sub>3</sub> (44 mmol/L). Whereas, NaCl solution containing the same Na<sup>+</sup> concentration was not effective. The mRNA for NBCn1 and NBCe1 were detected in microdissected Af-Art using reverse transcription-polymerase chain reaction and quantitative polymerase chain reaction. The Af-Art intracellular pH measured with 2',7'-bis-(2-carboxyethyl)-5-(and-6) carboxyfluorescein, acetoxymethyl ester increased significantly by 0.29±0.02 (n=6; <i>P</i><0.05) in the presence of NaHCO<sub>3</sub>, which was blunted by N-cyanosulphonamide compound (S0859) that is an inhibitor of the NBC family. After clamping the intracellular pH with 10 μM nigericin, changing the bath solution pH from 7.4 to 7.8 still dilates the Af-Art by 53±4% (n=7; <i>P</i><0.005) and increases NO generation by 22±3% (n=7; <i>P</i><0.005). Both pH-induced NO generation and vasodilation were blocked by L-NG-Nitroarginine Methyl Ester. NaHCO<sub>3</sub> increased NO generation in Af-Art by 19±4% (n=5; <i>P</i><0.005) and elevated glomerular filtration rate in conscious mice by 36% (233 versus 318 ul/min; n=9-10; <i>P</i><0.0001). S0859 and L-NG-nitroarginine methyl ester blocked NaHCO<sub>3</sub>-induced increases in NO generation and vasodilation. We conclude that NBCn1 and NBCe1 are expressed in Af-Art and that NaHCO<sub>3</sub> dilates Af-Art via NBCs mediated by NO that increases the glomerular filtration rate.
Effect of (3-aminopropyl) triethylsilane treatment on mechanical and thermal properties of alumina-filled epoxy composites (2011)

This article reports the effects of silane coupling agent (3-aminopropyl triethylsilane) on alumina particles, which were subsequently used as filler for an epoxy resin matrix. Fourier transform infrared spectroscopy was used to study the interaction between silane and alumina particles. The effectiveness of the coupling treatment was also correlated to the improvements on the composites properties. The effect of alumina loading on the properties was also investigated. Results from mechanical testing indicated improvements in terms of mechanical properties as a result of the coupling treatment. The morphology of fracture surfaces, from a three-point bend test, was examined using scanning electron microscopy. The coefficient of thermal expansion (CTE) suggests that through the addition of the alumina particle, the CTE value decreased accordingly. The thermal stability result, using thermogravimetric analysis, showed that treated alumina filled epoxy composite is much more stable for a given temperature.
Analysis of thin and thick Films (2012)

This chapter contains sections titled: Introduction Thin and Thick Layers: A Tentative Definition What Specific Information MS Techniques could Bring to thin and thick Films Analysis Compared to other Techniques? Main MS Techniques Applied to Thin/Thick Films Time of Sputtering/Speed of Acquisition Trade-Off Differences in Sputtering/Ionization Mechanisms between SIMS and GD-MS Pulse Shapes and how to Best Use Temporal Information for Pulsed Glow Discharge Mass Spectrometry Measurement and Data Interpretation in GD-TOFMS Practical Examples Conclusions List of Abbreviations References
Influence of cap weight on the motion of a Janus particle very near a wall (2020)

The dynamics of anisotropic nano- to micro scale colloidal particles in confined environments, either near neighboring particles or boundaries, is relevant to a wide range of applications. We utilized Brownian dynamics simulations to predict the translational and rotational fluctuations of a Janus sphere with a cap of nonmatching density near a boundary. The presence of the cap significantly impacted the rotational dynamics of the particle as a consequence of gravitational torque at experimentally relevant conditions. Gravitational torque dominated stochastic torque for a particle >1 μm in diameter and with a 20-nm-thick gold cap. Janus particles at these conditions sampled mostly cap-down or "quenched" orientations. Although the results summarized herein showed that particles of smaller diameter (<1 μm) with a thin gold coating (<5 nm) behave similarly to an isotropic particle, small increases in either particle diameter or coating thickness quenched the polar rotation of the particle. Histogram landscapes of the separation distance from the boundary and orientation observations of particles with larger diameters or thicker gold coatings were mostly populated with quenched configurations. Finally, the histogram landscapes were inverted to obtain the potential energy landscapes, providing a road map for experimental data to be interpreted.
Oxide-Free Actuation of Gallium Liquid Metal Alloys Enabled by Novel Acidified Siloxane Oils (2016)

Electrowetting and electrocapillarity of liquid metals have a long history, and a recent explosion of renewed interest. Liquid metals have electromagnetic properties and surface tensions (>500 mN/m) that enable new forms of reconfigurable devices. However, the only nontoxic option, gallium alloys, suffer from immediate formation of a semirigid surface oxide. Although acids or electrochemical reduction can remove this oxide, these approaches surround the gallium alloy in a fluid that is also electrically conducting, diminishing electromagnetic effectiveness and precluding electrowetting actuation. Reported here are acidified siloxanes that remove and prevent oxide formation. Importantly, the siloxane oil associatively incorporates hydrochloric or hydrobromic acids, is electrically insulating, is chemically stable, removes etching byproducts (including water), and allows robust electrowetting. This work opens up new opportunities for liquid metal reconfiguration, and is of fundamental interest due to the unexpected chemical stability of the acidified siloxanes and their application to other materials and surfaces.
Abstract S6-03: The prognosis of small HER2+ breast cancers: A meta-analysis of the randomized trastuzumab trials (2013)

Abstract Background Trastuzumab (T) combined with chemotherapy improves survival of women with HER2-positive breast cancer. Dual therapy with T and other HER-2 targeted agents (lapatinib/pertuzumab) has demonstrated improved efficacy compared to T alone in advanced breast cancer, and is in clinical trials in early stage disease. We hypothesize that subgroups of patients with HER2-positive tumors and limited tumor burden (tumor size and no or few positive lymph nodes) have a favorable outcome and would not be candidates for dual therapy. This study will analyze the disease-free survival (DFS)and overall survival (OS) of patients with early stage breast cancer treated with chemotherapy and T in the seminal randomized studies (RCTs). Methods RCTs were identified by a Medline search from 2004-2013. Trial groups from five phase III RCTs agreed to provide data. TrialNumberEligibilityRegimenNSABP B312130T1-3; pN1, pN2a, or pN3a; M0ACx4 then Tx4 VSACx4 then THx4 then Hx40wkNCCTG N98313505T1-3; pN1-2; M0ACx4 then Tx12wk VS ACTx4 then Hx52wk VS ACx4 then Tx12wk then Hx40wkHERA5102T1-3; pN1-2; M0Any (neo)adjuvant regimen, then H q3wk x1yr VS H q3wk x 2yr VS observationFinHER232T1-4; N0-3; M0Vx3 or T*x3 then FECx3 VS Vx3 or T*x3 with Hx9wk then FECx3PACS043010(528 HER2+)T1-3; pN1-2; M0FE(100)Cx6 VSFE(100)Cx6 then Hx1yr VS T*E(75) x6 VS T*E(75) x6 then Hx1yrA, adriamycin; C, cyclophosphamide; T, paclitaxel; T*, docetaxel; H, trastuzumab; P, carboplatin; F, 5-fluorouracil; E, epirubicin; Treatment regimen also included 5 years of hormonal therapy for ER positive patients. The ER+/HER2+ and the ER-/HER2+ cohorts will be analyzed separately. Patients with tumors 3 cm or smaller (T1a, T1b, T1c and &amp;gt;2 cm- 3cm) and 0, 1, 2 and 3 positive lymph nodes will be included. Initially a log rank test will be used to determine if the survival results are different by study. If there is no difference across studies, Kaplan-Meier survival curves with confidence intervals will be estimated. If there is a difference between studies, individual Kaplan-Meier curves will be estimated and the mean of the curves will be estimated along with confidence intervals using the adjusted standard deviation. Estimates at 3, 5 and 8 years will be presented as well as estimates of the event rates within each subpopulation defined by tumor size, number of positive lymph nodes, and ER status. Survival endpoints of DFS, and OS as defined in the original studies will be analyzed. Results and Conclusion Data from the five trials will be analyzed and an abstract provided prior to the meeting. Subgroups of patients with a very favorable outcome when treated with adjuvant chemotherapy and T will be identified. Our survival estimates might serve as benchmarks for future trials evaluating therapy reduction. Our results will complement those of the Dana Farber single arm multicenter trial in patients with “low risk” HER2-positive disease (APT trial; NCT00542451), being submitted to SABCS 2013. Citation Information: Cancer Res 2013;73(24 Suppl): Abstract nr S6-03.
Separation and identification of purine nucleosides in the urine of patients with malignant cancer by reverse phase liquid chromatography/electrospray tandem mass spectrometry (2009)

Urinary-modified nucleosides have a potential role as cancer biomarkers for a number of malignant diseases. High performance liquid chromatography (HPLC) was combined with full-scan mass spectrometry, MS/MS analysis and accurate mass measurements in order to identify purine nucleosides purified from urine. Potential purine nucleosides were assessed by their evident UV absorbance in the HPLC chromatogram and then further examined by the mass spectrometric techniques. In this manner, numerous modified purine nucleosides were identified in the urine samples from cancer patients including xanthine, adenosine, N1-methyladenosine, 5'-deoxy-5'-methylthioadenosine, 2-methyladenosine, N6-threonylcarbamoyladenosine, inosine, N1-methylinosine, guanosine, N1-methylguanosine, N7-methylguanine, N2-methylguanosine, N2,N2-dimethyguanosine, N2,N2,N7-trimethylguanosine. Furthermore, a number of novel purine nucleosides were tentatively identified via critical interpretation of the combined mass spectrometric data including N3-methyladenosine, N7-methyladenine, 5'-dehydro-2'-deoxyinosine, N3-methylguanine, O6-methylguanosine, N1,N2,N7-trimethylguanosine, N1-methyl-N2-ethylguanosine and N7-methyl-N1-ethylguanosine.
A Diagnostic Technique for Particle Characterization Using Laser Light Extinction (2015)

Increased operations of aircraft, both commercial and military in hostile desert environments has increased the risk of micro sized particle ingestion into engines. The probability of increased sand and dust ingestion results in increased life cycle costs in addition to increased potential for performance loss. Thus the ability to accurately assess the amount of inlet debris would be useful for engine diagnostics and prognostic evaluation. Previous engine monitoring studies were based on particle measurements performed a posteriori. Thus there exists a need for in situ quantification of ingested particles. This paper describes the initial development of a line-of-sight optical technique to characterize the ingested particles at concentrations similar to those experienced by aircraft in brownout conditions using laser extinction with the end goal of producing an onboard aircraft diagnostic sensor. By measuring the amount of light that is transmitted due to the effects of scattering and absorption in the presence of particles over a range of concentrations, a relationship between particle diameters and the laser light extinction was obtained. This relationship was then used to obtain information on diameters and number densities of ingested particles. The particle size range of interest was chosen to be between 1–10 μm and the size distribution function was assumed to be lognormal. Tests were performed on polystyrene latex spheres of sizes 1.32 μm, 3.9 μm and 5.1 μm in water dispersions to measure diameters and concentrations. Measurements were performed over multiple wavelengths to obtain information on the size distribution and number density of particles. Results of tests presented in this paper establish the validity of the laser extinction technique to provide real time information of ingested particles and will serve as an impetus to carry out further research using this technique to characterize particles.
Ps−emission from Na coated W(100) surfaces (2011)

The emission of positronium negative ions from a Na coated W(100) surface has been studied. The emission efficiency was found to increase with time following Na deposition to a maximum of 1.4% after 6 × 104 s. The efficiency then gradually decreased but was still higher than 0.5% after 3 days.
Forward-link power control utilizing neighboring-cell pilot power for DS-CDMA cellular systems (2002)

This paper proposes for DS-CDMA cellular systems a forward-link power control scheme utilizing neighboring-cell pilot power in order to improve the quality of forward traffic channels and to increase traffic capacity. In this scheme, each mobile station measures pilot powers received from neighboring cells. Transmission power of the forward traffic channel is then controlled by base stations according to the pilot powers measured at the respective mobile station, and uniform service quality is provided to all channels. Simulation results show that the proposed scheme reduces outage and forced termination probabilities with a pilot power error of 1.5 dB, and increases capacity for the forced termination probability of 1% by 5% and 60% in the case of soft handover and hard handover.
Gaussian beams representation based on periodic frames for radiation from cylindrical apertures (2004)

A formulation is introduced for periodic windowed Fourier transform (WFT) frames. Such frames are then used to represent fields on cylindrical surfaces. Through phase matching at the surface, fields radiated from localized Gaussian windows are expressed in the form of general Gaussian beams, leading to a representation of radiated fields as sums of beam propagators. Such representations are presented on test cases, and can be most useful in representing fields in complex environments, around linear sources or obstacles.
Development of a Practical Synthesis of 4-[6-(Morpholinomethyl)-pyridin-3-yl]naphthalen-1-amine, a Key Intermediate for the Synthesis of BIRB 1017, a Potent p38 MAP Kinase Inhibitor (2013)

The development of synthetic routes to 4-[6-(morpholinomethyl)pyridin-3-yl]naphthalen-1-amine, the key intermediate of p38 MAP kinase inhibitor BIRB 1017, via (1) trialkylmagnesium ate complex mediated metalation and borylation followed by Suzuki coupling reactions and (2) pyridine-ring formation from a vinamidinium salt, is described.
Process for obtaining silicon from fluosilicic acid (2023)

Process for producing low cost, high purity solar grade Si wherein a reduction reaction, preferably the reduction of SiF.sub.4, by an alkali metal (Na preferred) is carried out by jetting a spray of reactants into a reaction chamber at a rate and temperature which causes the reaction to take place far enough away from the entry region to avoid plugging of reactants at the entry region and wherein separation in the melt is carried out continuously from the reaction and the Si can be cast directly from the melt.
Compact 2D laser beam scanner with fan laserarray and Si micromachined microscanner (1997)

The first demonstration of a compact 2D laser beam scanning system using a beam steering fan diode laser array and an Si surface micromachined microscanner is reported. The system is capable of reading and writing operations that detect or generate arbitrary patterns with 7 × 10 pixel resolution. The system demonstrates the viability of these new technologies for applications such as laser scanners for remote sensing and barcode scanning, multi-beam laser printers, and multi-line write 2D laser displays.
Cognition and fatigue in multiple sclerosis: Potential effects of medications with central nervous system activity (2006)

To evaluate the potential effects of medications with central nervous system (CNS) activity on cognitive function and fatigue in multiple sclerosis (MS), we performed a retrospective analysis of medication use among 70 subjects with MS who were participating in a clinical trial for evaluation of the effects of yoga and exercise programs on cognition and fatigue. Among these MS subjects, 74% were taking at least one potentially CNS-active medication. These 70 subjects were divided into two groups: those taking at least one CNS-active medication (n = 52) and those not on any medications with potential CNS activity (n = 18). We compared assessments of cognitive function and fatigue using an analysis of covariance. MS subjects on CNS-active medication had greater impairment on measures of processing speed, sustained attention, and fatigue than those not on these medications. While these findings do not establish a causal relationship between medication use and cognitive impairment and fatigue, the data indicate that researchers need to control for use of CNS-active medications when conducting studies of cognitive impairment and fatigue in MS subjects.
K562 cells produce and respond to human erythroid-potentiating activity (1988)

Human erythroid-potentiating activity (EPA) is a 28,000 mol wt glycoprotein that stimulates the growth of erythroid progenitors in vitro and enhances colony formation by the K562 human erythroleukemia cell line. EPA has potent protease inhibitory activity, and is also referred to as tissue inhibitor of metalloproteinases (TIMP). We observed that colony formation by K562 cells in semi-solid medium containing reduced fetal calf serum (FCS) is not directly proportional to the number of cells plated, suggesting production of autostimulatory factors by K562 cells. Using radioimmunoprecipitation and a bioassay for EPA, medium conditioned by K562 cells was found to contain high levels of biologically active EPA; Northern hybridization analysis confirmed the expression of EPA mRNA. Radiolabeled EPA was used to identify cell surface receptors on K562 cells. Together, these results suggest that EPA may act as an autocrine growth factor for K562 cells.
Multiple forgery attacks against Message Authentication Codes. (2005)

Some message authentication codes (MACs) are vulnerable to multiple forgery attacks, in which an attacker can gain information that allows her to succeed in forging multiple message/tag pairs. This property was first noted in MACs based on universal hashing, such as the Galois/Counter Mode (GCM) of operation for block ciphers. However, we show that CBC-MAC and HMAC also have this property, and for some parameters are more vulnerable than GCM. We present multiple-forgery attacks against these algorithms, then analyze the security against these attacks by using the expected number of forgeries. We compare the different MACs using this measure. This document is a pre-publication draft manuscript.
Transmission properties of nonlinear multimode waveguide arrays (2003)

Multimode capillary waveguide arrays containing reverse-saturable absorbers exhibit an enhancement of the nonlinear response. Experimental data are modeled by the effect of partial mode filling, influenced by fill fraction and capillary diameter.
Photovoltaic cell with electrolyte redox system of hole-conducting compound (1998)

Patent number: 97-19711713-19711713 Reference LPI-PATENT-1998-002Search for this record at the European Patent Office Record created on 2006-02-21, modified on 2017-05-12
Characterization of hepatic progenitors from human fetal liver during second trimester (2008)

Our study suggests that these EpCAM +ve cells can be used as hepatic progenitors for cell transplantation with a minimum risk of alloreactivity and these cells may serve as a potential source for enrichment of hepatic progenitor.