gebhart/patrepeval huggingface dataset:
Digital Holographic Microscopy, a new imaging technology applied to nanotechnology and life (2005)

Keywords: [MVD] Reference LOA-CONF-2005-006 Record created on 2009-07-20, modified on 2017-12-10
Antinociceptive properties of an isoform-selective inhibitor of Nav1.7 derived from saxitoxin in mouse models of pain (2020)

The voltage-gated sodium channel Nav1.7 is highly expressed in nociceptive afferents and is critically involved in pain signal transmission. Nav1.7 is a genetically validated pain target in humans because loss-of-function mutations cause congenital insensitivity to pain and gain-of-function mutations cause severe pain syndromes. Consequently, pharmacological inhibition has been investigated as an analgesic therapeutic strategy. We describe a small molecule Nav1.7 inhibitor, ST-2530, that is an analog of the naturally occurring sodium channel blocker saxitoxin. When evaluated against human Nav1.7 by patch-clamp electrophysiology using a protocol that favors the resting state, the Kd of ST-2530 was 25 ± 7 nM. ST-2530 exhibited greater than 500-fold selectivity over human voltage-gated sodium channel isoforms Nav1.1-Nav1.6 and Nav1.8. Although ST-2530 had lower affinity against mouse Nav1.7 (Kd = 250 ± 40 nM), potency was sufficient to assess analgesic efficacy in mouse pain models. A 3-mg/kg dose administered subcutaneously was broadly analgesic in acute pain models using noxious thermal, mechanical, and chemical stimuli. ST-2530 also reversed thermal hypersensitivity after a surgical incision on the plantar surface of the hind paw. In the spared nerve injury model of neuropathic pain, ST-2530 transiently reversed mechanical allodynia. These analgesic effects were demonstrated at doses that did not affect locomotion, motor coordination, or olfaction. Collectively, results from this study indicate that pharmacological inhibition of Nav1.7 by a small molecule agent with affinity for the resting state of the channel is sufficient to produce analgesia in a range of preclinical pain models.
Atypical Gilles de la Tourette Syndrome With β-Mannosidase Deficiency (2006)

This case illustrates the phenotypic variability of inborn errors of metabolism in adults and demonstrates the need to screen inborn errors of metabolism in atypical Gilles de la Tourette syndrome.
Low sonic boom design and performance of a Mach 2.4/1.8 overland high speed civil transport (1992)

This paper describes the design features of a Douglas Mach 2.4/1.8 Low Sonic Boom High Speed Civil Transport (HSCT) configuration developed for NASA. The configuration is designed to fly over water at Mach 2.4 for highest productivity and economic worth, and fly over land at Mach 1.8 with reduced sonic boom loudness.
Efficient method for the total asymmetric synthesis of the isomers of .beta.-methyltyrosine (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEfficient method for the total asymmetric synthesis of the isomers of .beta.-methyltyrosineErnesto Nicolas, K. C. Russell, J. Knollenberg, and Victor J. HrubyCite this: J. Org. Chem. 1993, 58, 26, 7565–7571Publication Date (Print):December 1, 1993Publication History Published online1 May 2002Published inissue 1 December 1993https://pubs.acs.org/doi/10.1021/jo00078a042https://doi.org/10.1021/jo00078a042research-articleACS PublicationsRequest reuse permissionsArticle Views496Altmetric-Citations49LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Crashworthiness of Magnesium Sheet Structures (2013)

A hollow rectangular profile, as an example of a typical structural component made of magnesium alloy sheets has been built, tested and evaluated in order to assess its behaviour during axial crushing. The profiles were joined from plane sheets of AZ31 and ZE10, respectively, by laser beam welding and were then tested in compression. Numerical simulations have been conducted to understand the complex interplay between hardening characteristics of the materials under investigation, profile cross-section variation and energy absorption. The results from the compression testing of the profiles show that the welds are not the source of damage initiation and failure. The performance of the magnesium profiles in terms of dissipated specific energy is confirmed for small and intermediate displacements to be comparable to that of aluminium profiles. For large displacements, however, the shear-type failure mode of magnesium causes a sharp drop of the crushing force and thus limits the energy absorption. These findings demonstrate the requirement for an alloy and wrought magnesium process development specifically for crash applications which aims at progressive hardening along with high ductility for improving the bending and shear behaviour.
Smoothing a current-carrying atomic mirror (1999)

We have measured the magnitude and roughness of the reflecting potential produced by a current-carrying magnetic mirror from which cold rubidium atoms have been bounced at normal incidence. By varying the current in the mirror, a study of the potential barrier from which the atoms in different magnetic substates bounce has been made. A combination of time-of-flight and imaging techniques allowed us to study the roughness of the reflecting potential. We have demonstrated that the observed roughness can be reduced by additional wires to compensate for the finite-size effects, and by careful control of the direction of the magnetic bias field.
The New IEEE-754 Standard for Floating Point Arithmetic (2008)

The current IEEE-754 floating point standard was adopted 23 years ago. IEEE chartered a committee to revise the standard to include new common practice in floating point arithmetic, to incorporate decimal floating point into the standard, and to address the issue of reproducible results. This talk will visit these issues, based on the current work of the IEEE-754 revisions committee, which expects that a new standard will be adopted sometime in 2008.
Assessing output measurement optimally in physiological system identification by a design measure approach (1986)

The design of optimal experiments in physiological system identification may be very difficult to perform or even not always required, especially in the early model building/validation phase. It is however important to understand the role that the design variables exert on the precision of parameter estimates and to have guidelines for improving the experiments. For this purpose we propose here an approach based on the Kiefer-Wolfowitz theorem for assessing the optimality of output measurements for given system/experiment models, which does not require the explicit solution of the optimal sampling schedule design problem.
ChemInform Abstract: INTEGRATED SYSTEMS FOR WATER CLEAVAGE BY VISIBLE LIGHT; SENSITIZATION OF TITANIUM DIOXIDE PARTICLES BY SURFACE DERIVATIZATION WITH RUTHENIUM COMPLEXES (1984)

Abstract Bestrahlung saurer Lösungen (pH 2, HCl) von [RuL3]C12 in Gegenwart dispergierter TiO 2 ‐Teilchen (Anatas) bei 100°C führt zum Verlust eines L‐Liganden von RuLä+ und zur chemischen Fixierung von RuLg an der TiOg‐Oberfläche durch Bildung von Ru‐O‐ Ti‐Bindungen (L: Diisopropyl‐2,2′‐bipyridin‐4,4′‐dicarboxylat).
Circuit Simulation of Magnetization Dynamics and Spin Transport (2014)

In this paper, compact circuit models for spintronic devices have been developed by manipulating the underlying physical equations. We have simulated, via circuit simulation: 1) the magnetization dynamics governed by the Landau-Lifshitz-Gilbert (LLG) equation and 2) the spin transport physics governed by the spin drift-diffusion equation. The models have been validated using numerical and analytical solutions of the LLG equation and the spin drift-diffusion equations, respectively. Simulations of an all-spin logic device demonstrate the applications of the developed models in device and circuit simulation.
Outage and Diversity of Linear Receivers in Flat-Fading MIMO Channels (2007)

This correspondence studies linear receivers for multiple-input-multiple-output (MIMO) channels under frequency-nonselective (flat) quasi-static Rayleigh fading. The outage probability and diversity gain of minimum mean square error (MMSE) and zero forcing (ZF) receivers are investigated. Assuming M. transmit and JV receive antennas, the ZF receiver always has diversity N - M + 1, unlike the MMSE receiver which may exhibit a rate-dependent behavior. Under separate spatial encoding, where the parallel data streams are not jointly encoded, MMSE is no better than ZF in terms of diversity. Under joint spatial encoding, the MMSE receiver achieves diversity MN at low spectral efficiencies but has diversity only M - N + 1 at high spectral efficiencies. These results are established via simulations and an outline for the corresponding analysis is presented.
ChemInform Abstract: Enantioselective Synthesis of Cyclic Sulfamidates by Using Chiral Rhodium‐Catalyzed Asymmetric Transfer Hydrogenation. (2010)

Abstract The reaction applies a HCOOH/NEt 3 mixture as a hydrogen source and a chiral Rh‐complex as a catalyst.
[Renal malakoplakia: prognostic aspects]. (1999)

We report the changes in imaging findings over a 5 year period of a patient with unilateral malakoplakia involving renal parenchyma and pelvis. The imaging findings of malakoplakia will be reviewed.
The Drug Annotations Series (2013)

ADVERTISEMENT RETURN TO ISSUEEditorialNEXTThe Drug Annotations SeriesJeff Zablocki* and Carlos Garcia-Echeverria*View Author Information Gilead Sciences Sanofi*For J.Z.: e-mail, [email protected] or [email protected]. J.Z. is a Senior Director at Gilead Sciences where he leads a team of scientists working on targets in multiple therapeutic areas (cardiovascular, oncology, inflammation, and antiviral). He has served as the ACS MEDI Chair and is an ACS Fellow.*For C.G.-E.: e-mail, [email protected]. C.G.-E. is Deputy Head Oncology Drug Discovery and Preclinical Development at Sanofi. He supervises the drug discovery, medicinal chemistry and in vivo pharmacology functions of the Sanofi oncology business unit. He is an ACS Fellow.Cite this: J. Med. Chem. 2013, 56, 17, 6541Publication Date (Web):September 3, 2013Publication History Published online3 September 2013Published inissue 12 September 2013https://pubs.acs.org/doi/10.1021/jm401297rhttps://doi.org/10.1021/jm401297reditorialACS PublicationsCopyright © 2013 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views6707Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (122 KB) Get e-AlertscloseSUBJECTS:Drug discovery,Mechanisms of action,Medicinal chemistry,Pharmaceuticals,Therapeutics Get e-Alerts
Asymmetric patella resurfacing in total knee arthroplasty. (2000)

Three hundred consecutive primary, cemented, condylar total knee arthroplasties (TKAs) were reviewed for the presence of asymmetric patella resurfacing using a postoperative Merchant or sunrise patellar radiograph. Twenty-one knees in 14 patients were found to have the patella asymmetrically resurfaced. Asymmetric resurfacing typically involved the inadvertent preferential resurfacing of the lateral facet with underresection of bone from the medial patellar facet. All patients underwent follow-up for a minimum of 5 years, with a mean follow-up of 7.5 years. Of the 21 knees, 3 revisions were required for patellar complications. One patellar component was loose on radiographs and there was marked patellofemoral pain in 6 knees. Overall, 11 of 21 knees (52%) underwent revision or were recommended for revision for patellar complications or had anterior knee pain that limited activities. Inadvertent asymmetric patella resurfacing using the kinematic condylar implant adversely affects the outcome after TKA.
Multiple in vivo phosphorylated tyrosine phosphatase SHP-2 engages binding to Grb2 via tyrosine 584. (1996)

SHP-2 (also named PTP1D, syp, or SH-PTP2) has been identified as a phosphotyrosine phosphatase comprising two src-homology-2 (SH2) domains. Upon growth factor stimulation, SHP-2 becomes tyrosine phosphorylated, thereby increasing its catalytic activity. Here, we identified SHP-2 to be phosphorylated on multiple tyrosine residues in response to different stimuli and unmasked the carboxyl-terminal tyrosine 584 as a major phosphorylation site in human cell lines. Tyrosine 584 shares, together with tyrosine 546, the consensus sequence pY-X-N-X, a characteristic of potential binding sites for the SH2 domain of growth factor receptor-bound protein 2 (Grb2). We show here that mutation of tyrosine 584, but not tyrosine 546, to phenylalanine totally abolished the binding of Grb2 to SHP-2. By using a systematic mutagenesis approach, phosphorylation of additional tyrosines in each of the SH2 domains of SHP-2 was detected after coexpression of epidermal growth factor receptor, but not after coexpression of platelet-derived growth factor receptor, whereas tyrosine 263 located in the interspace between SH2 and catalytic domain appears to be exclusively recognized by platelet-derived growth factor receptor. Immunoprecipitation of SHP-2 from a panel of mammary carcinoma cell lines copurifies several tyrosine phosphorylated proteins; the most prominent band has an apparent molecular weight of M(r) 115,000.
BGP UPDATE for SDWAN Edge Discovery (1999)

The document describes encoding of BGP UPDATE messages for the SDWAN
edge node discovery. In the context of this document, BGP Route
Reflectors (RR) is the component of the SDWAN Controller that receives
the BGP UPDATE from SDWAN edges and in turns propagates the
information to the intended peers that are authorized to communicate
via the SDWAN overlay network.
Supercapacitor Electrical and Thermal Modeling, Identification, and Validation for a Wide Range of Temperature and Power Applications (2015)

Supercapacitors benefit from unique features including high power density, long cycle life, wide temperature operation range, durability in harsh environments, efficient cycling, and low maintenance cost. This paper presents a validated lumped and computationally efficient electrical and thermal model for a cylindrical supercapacitor cell. The electrical model is a two-state equivalent electric circuit model with three parameters that are identified using temporal experiments. The dependence of the parameters on the state of charge, current direction and magnitude (20-200 A), and temperatures ranging from -40 °C to 60 °C is incorporated in the model. The thermal model is a linear 1-D model with two states. The reversible heat generation which is significant in double-layer capacitors is included in the thermal model. The coupling of the two models enables tuning of the temperature-dependent parameters of the electrical model in real time. The coupled electrothermal model is validated using real-world duty cycles at subzero and room temperatures with root-mean-square errors of (82 mV-87 mV) and (0.17 °C-0.21 °C) for terminal voltage and temperature, respectively. This accurate model is implementable in real-time power applications and also thermal management studies of supercapacitor packs.
SARS-CoV-2 and interferon blockade (2020)

The response to viral infection generally includes an activation of the adaptive immune response to produce cytotoxic T cells and neutralizing antibodies. We propose that SARS-CoV-2 activates the innate immune system through the renin-angiotensin and kallikrein-bradykinin pathways, blocks interferon production and reduces an effective adaptive immune response. This model has therapeutic implications.
A natural mutation between SARS-CoV-2 and SARS-CoV determines neutralization by a cross-reactive antibody (2020)

Epitopes that are conserved among SARS-like coronaviruses are attractive targets for design of cross-reactive vaccines and therapeutics. CR3022 is a SARS-CoV neutralizing antibody to a highly conserved epitope on the receptor binding domain (RBD) on the spike protein that is able to cross-react with SARS-CoV-2, but with lower affinity. Using x-ray crystallography, mutagenesis, and binding experiments, we illustrate that of four amino acid differences in the CR3022 epitope between SARS-CoV-2 and SARS-CoV, a single mutation P384A fully determines the affinity difference. CR3022 does not neutralize SARS-CoV-2, but the increased affinity to SARS-CoV-2 P384A mutant now enables neutralization with a similar potency to SARS-CoV. We further investigated CR3022 interaction with the SARS-CoV spike protein by negative-stain EM and cryo-EM. Three CR3022 Fabs bind per trimer with the RBD observed in different up-conformations due to considerable flexibility of the RBD. In one of these conformations, quaternary interactions are made by CR3022 to the N-terminal domain (NTD) of an adjacent subunit. Overall, this study provides insights into antigenic variation and potential cross-neutralizing epitopes on SARS-like viruses.
Three Novel Antisense Overlapping Genes in E. coli O157:H7 EDL933 (2022)

The abundance of long overlapping genes in prokaryotic genomes is likely to be significantly underestimated. To date, only a few examples of such genes are fully established. Using RNA sequencing and ribosome profiling, we found expression of novel overlapping open reading frames in Escherichia coli O157:H7 EDL933 (EHEC). Indeed, the overlapping candidate genes are equipped with typical structural elements required for transcription and translation, i.e., promoters, transcription start sites, as well as terminators, all of which were experimentally verified. Translationally arrested mutants, unable to produce the overlapping encoded protein, were found to have a growth disadvantage when grown competitively against the wild type. Thus, the phenotypes found imply biological functionality of the genes at the level of proteins produced. The addition of 3 more examples of prokaryotic overlapping genes to the currently limited, yet constantly growing pool of such genes emphasizes the underestimated coding capacity of bacterial genomes. <b>IMPORTANCE</b> The abundance of long overlapping genes in prokaryotic genomes is likely to be significantly underestimated, since such genes are not allowed in genome annotations. However, ribosome profiling catches mRNA in the moment of being template for protein production. Using this technique and subsequent experiments, we verified 3 novel overlapping genes encoded in antisense of known genes. This adds more examples of prokaryotic overlapping genes to the currently limited, yet constantly growing pool of such genes.
Magnetic resonance imaging study of complex flow of viscoelastic fluids (2010)

Abstract NMR imaging techniques have been applied to investigate complex fluid dynamics in pipes and in annular flow conditions. In this application, aimed to simulate drilling and production operations, two lines have been set up to reproduce, at small laboratory scale, respectively, a pipe flow and an annular flow that are typical flow geometries in oilfield wells during drilling. NMR imaging measurements have been performed inside a horizontal‐bore magnet with a fluid flow assured in a velocity range of 10–100 cm s −1 . The studies were dedicated to investigate the different flow regimes associated to circulating viscous polymer solutions applied in drilling mud formulations. Early transitions from laminar to turbulent regime were observed at very low Reynolds number (in a range between 300 and 500, to be compared with the theoretical transition value greater than 2100). © 2010 American Institute of Chemical Engineers AIChE J, 2011
Optimizing Nanoplasmonic Biosensor Sensitivity with Orientated Single Domain Antibodies (2015)

Localized surface plasmon resonance (LSPR) spectroscopy and imaging are emerging biosensor technologies which tout label-free biomolecule detection at the nanoscale and ease of integration with standard microscopy setups. The applicability of these techniques can be limited by the restrictions that surface-conjugated ligands must be both sufficiently small and orientated to meet analyte sensitivity requirements. We demonstrate that orientated single domain antibodies (sdAb) can optimize nanoplasmonic sensitivity by comparing three anti-ricin sdAb constructs to biotin-neutravidin, a model system for small and highly orientated ligand studies. LSPR imaging of electrostatically orientated sdAb exhibited a ricin sensitivity equivalent to that of the biotinylated LSPR biosensors for neutravidin. These results, combined with the facts that sdAb are highly stable and readily produced in bacteria and yeast, build a compelling case for the increased utilization of sdAbs in nanoplasmonic applications.
Characterization of a monoclonal antibody specific for Brucella smooth lipopolysaccharide and development of a competitive enzyme-linked immunosorbent assay to improve the serological diagnosis of brucellosis (1996)

The reactivity of monoclonal antibody (MAb) 12G12 was analyzed in regard to the main biovars of Brucella species and some members of the families Enterobacteriaceae and Vibrionaceae which present serological cross-reactions with the smooth lipopolysaccharide (S-LPS) of Brucella species. This MAb was strictly directed against the common specific epitope of the Brucella S-LPS. It recognized all of the smooth Brucella strains and biovars except B. suis biovar 2. In order to improve the specificity of the serological diagnosis of brucellosis, a competitive enzyme-linked immunosorbent assay (cELISA) was developed with the horseradish peroxidase-conjugated MAbs 12G12 and S-LPS of B. melitensis Rev1. The specificity of the cELISA was analyzed with 936 serum samples from healthy cattle. The assay was evaluated with sera from heifers (n = 18) experimentally infected with B. abortus 544. After infection, the performance of the cELISA was in agreement with those of the complement fixation test and the rose Bengal plate test. Finally, the specificity of the assay was also evaluated in regard to false-positive serological reactions by using sera from heifers experimentally infected with Yersinia enterocolitica 0:9 (n = 4) and with field sera presenting false-positive reactions (n = 74). The specificity of the cELISA was greater than the specificities of the complement fixation test and the rose Bengal plate test. Indeed, the new assay detected only 31 of the 101 false-positive serum samples detected by at least one serological test.