gebhart/patrepeval huggingface dataset:
Solar fuels (2013)

The generation of hydrogen from water and sunlight may provide the clean, renewable fuel that solves our planet's growing demand for energy. However, for any approach to be practical, we will need to develop robust catalysts containing earthabundant elements for the efficient reduction of protons to H 2 and oxidation of water to O 2 .
MDM2 E3 ligase-mediated ubiquitination and degradation of HDAC1 in vascular calcification (2016)

Vascular calcification (VC) is often associated with cardiovascular and metabolic diseases. However, the molecular mechanisms linking VC to these diseases have yet to be elucidated. Here we report that MDM2-induced ubiquitination of histone deacetylase 1 (HDAC1) mediates VC. Loss of HDAC1 activity via either chemical inhibitor or genetic ablation enhances VC. HDAC1 protein, but not mRNA, is reduced in cell and animal calcification models and in human calcified coronary artery. Under calcification-inducing conditions, proteasomal degradation of HDAC1 precedes VC and it is mediated by MDM2 E3 ubiquitin ligase that initiates HDAC1 K74 ubiquitination. Overexpression of MDM2 enhances VC, whereas loss of MDM2 blunts it. Decoy peptide spanning HDAC1 K74 and RG 7112, an MDM2 inhibitor, prevent VC in vivo and in vitro. These results uncover a previously unappreciated ubiquitination pathway and suggest MDM2-mediated HDAC1 ubiquitination as a new therapeutic target in VC.
A high‐performance matrix‐assisted laser desorption/ionization orthogonal time‐of‐flight mass spectrometer with collisional cooling (2003)

A high-performance orthogonal time-of-flight (TOF) mass spectrometer was developed specifically for use in combination with a matrix-assisted laser desorption/ionization (MALDI) source. The MALDI source features an ionization region containing a buffer gas with variable pressure. The source is interfaced to the TOF section via a collisional focusing ion guide. The pressure in the source influences the rate of cooling and allows control of ion fragmentation. The instrument provides uniform resolution up to 18,000 FWHM (full width at half maximum). Mass accuracy routinely achieved with a single-point internal recalibration is below 2 ppm for protein digest samples. The instrument is also capable of recording spectra of samples containing compounds with a broad range of masses while using one set of experimental conditions and without compromising resolution or mass accuracy.
Front Matter: Volume 9744 (2016)

This PDF file contains the front matter associated with SPIE Proceedings Volume 9744, including the Title Page, Copyright information, Table of Contents, Introduction (if any), and Conference Committee listing.
Magnesium in Methanol (Mg / MeOH) in Organic Syntheses (2004)

Magnesium in methanol(Mg / MeOH) system is an extremely versatile, efficient, economical and convenient reducing agent for various reactions useful for organic synthesis such as reductive cyclization, reductive elimination, reductive cleavage, reduction of a conjugated double bond, desulfonylation, and reduction of various functional groups. This comprehensive review is intended to highlight the use of Mg / MeOH in each of these organic transformations.
A 4.4-$\mu$W Wake-Up Receiver Using Ultrasound Data (2013)

In wireless sensor nodes, using a wake-up receiver to duty-cycle the main receiver significantly reduces the overall power consumption of the node, as long as the power consumption of the always-ON wake-up receiver is very low. We demonstrate line-of-sight ultrasound data communications for ultra-low power wireless wake-up in sensor networks. The 65-nm CMOS 0.6-V receiver prototype achieves a BER of better than 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-3</sup> measured for a 250-bps free-space 8.6-m link in an indoor environment while dissipating only 4.4 μ W and requiring only - 18 dBm electrical power to be delivered to the transmit transducer.
Pentapeptide repeat proteins QnrB1 and AlbG require ATP hydrolysis to rejuvenate poisoned gyrase complexes (2020)

ABSTRACT DNA gyrase, a type II topoisomerase found predominantly in bacteria, is the target for a variety of “poisons”, namely natural product toxins (e.g. albicidin. microcin B17) and clinically important synthetic molecules (e.g. fluoroquinolones). Resistance to both groups can be mediated by pentapeptide repeat proteins (PRPs). Despite long-term studies, the mechanism of action of these protective PRPs is not known. We compared activities of two such proteins, QnrB1 and AlbG in vitro . Each of them provided specific protection against its cognate toxin (fluoroquinolone or albicidin), which strictly required ATP hydrolysis by gyrase. Through a combination of fluorescence anisotropy, pull-downs and photocrosslinking we show that QnrB1 binds to the GyrB protein. We further probed the QnrB1 binding site using site-specific incorporation of a photoreactive amino acid and mapped strong and specific crosslinks to the N-terminal ATPase/transducer domain. We propose a model in which protective PRPs bind to the enzyme as T-segment DNA mimics to promote dissociation of the bound poison molecule.
Discovery of potent and selective PI3Kγ inhibitors via elaboration of a designed fragment and identification of key metabolites (2020)

The phosphoinositide 3-kinases (PI3Ks) catalyze the phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) as part of the canonical PI3K/AKT/mTOR pathway. Recently, the class I isoform PI3Kγ has been identified as an immuno-oncology target because it has been shown to exert control on the tumor microenvironment primarily through effects in macrophages. Herein, we report
Chitosan: A promising polymer for cartilage repair and viscosupplementation (2017)

Osteoarthritis (OA) is a painful, degenerative and inflammatory disease that affects the entire synovial joints. Nowadays, no cure exists, and the pharmacological treatments are limited to symptoms alleviation. There is a need for a new efficient and safe treatment. Viscosupplementation is a process that aims to restore the normal rheological properties of synovial fluid. For the past years, hyaluronic acid was usually used but this molecule has some limitations including the short residency time in joint cavity. Recently, in vitro studies have suggested that chitosan could promote the expression of cartilage matrix components and reduce inflammatory and catabolic mediator's production by chondrocytes. In vivo, chitosan prevented cartilage degradation and synovial membrane inflammation in OA induced rabbit model. Several studies have also shown that chitosan could induce chondrogenic differentiation of mesenchymal stem cells. Therefore, chitosan is an interesting polymer to design scaffold and hydrogel for cartilage lesion repair, cells transplantation, sustained drug release and viscosupplementation.
Ge and Ge-rich Group IV Alloys on Si for Photonic Device Applications (2009)

Dialysis patients had a high incidence of atrial fibrillation. This risk was largely segregated among those with established risk factors for atrial fibrillation, and hemodialysis patients. Use of coumadin was associated with improved survival among patients later hospitalized for atrial fibrillation.
Inversion formula with hypergeometric polynomials and its application to an integral equation (2019)

For any complex parameters $x$ and $ν$, we provide a new class of linear inversion formulas $T = A(x,ν) \cdot S \Leftrightarrow S = B(x,ν) \cdot T$ between sequences $S = (S_n)_{n \in \mathbb{N}^*}$ and $T = (T_n)_{n \in \mathbb{N}^*}$, where the infinite lower-triangular matrix $A(x,ν)$ and its inverse $B(x,ν)$ involve Hypergeometric polynomials $F(\cdot)$, namely $$ \left\{ \begin{array}{ll} A_{n,k}(x,ν) = \displaystyle (-1)^k\binom{n}{k}F(k-n,-nν;-n;x), \\ B_{n,k}(x,ν) = \displaystyle (-1)^k\binom{n}{k}F(k-n,kν;k;x) \end{array} \right. $$ for $1 \leqslant k \leqslant n$. Functional relations between the ordinary (resp. exponential) generating functions of the related sequences $S$ and $T$ are also given. These new inversion formulas have been initially motivated by the resolution of an integral equation recently appeared in the field of Queuing Theory; we apply them to the full resolution of this integral equation. Finally, matrices involving generalized Laguerre polynomials polynomials are discussed as specific cases of our general inversion scheme.
ChemInform Abstract: A New Multicomponent Synthesis of 1,2,3,4‐Tetrahydroquinolines. (1997)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Formulation Study for Lansoprazole Fast-disintegrating Tablet. I.Effect of Compression on Dissolution Behavior (2003)

Lansoprazole fast-disintegrating tablet (LFDT) is a new patient-friendly formulation of lansoprazole. Since lansoprazole is an antiulcer agent and is unstable under acidic conditions, we have developed LFDT as an orally disintegrating tablet containing enteric-coated microgranules. The effect of compression on dissolution behavior was investigated, as compression affected cleavage and crushing of the enteric layer. To decrease cleavage and crushing of the enteric layer, the effects of the combined ratio of methacrylic acid copolymer dispersion to ethyl acrylate-methyl methacrylate copolymer dispersion and the concentration of triethyl citrate on the dissolution in the acid stage and the dissolution in the buffer stage were evaluated. By adjusting the ratio of methacrylic acid copolymer dispersion to ethyl acrylate-methyl methacrylate copolymer dispersion to 9 : 1 and adding a 20% triethyl citrate concentration, sufficient flexibility of the enteric layer and sufficient stability against compression forces were achieved. Agglomeration of enteric-coated microgranules during the coating process was decreased at the optimized concentration of triethyl citrate and glyceryl monostearate. We compared the absorption properties of LFDT and lansoprazole capsules in dogs. The absorption profiles of LFDT were similar to those of lansoprazole capsules.
FLAP -- A Federated Learning Framework for Attribute-based Access Control Policies (2020)

Technology advances in areas such as sensors, IoT, and robotics, enable new collaborative applications (e.g., autonomous devices). A primary requirement for such collaborations is to have a secure system which enables information sharing and information flow protection. Policy-based management system is a key mechanism for secure selective sharing of protected resources. However, policies in each party of such a collaborative environment cannot be static as they have to adapt to different contexts and situations. One advantage of collaborative applications is that each party in the collaboration can take advantage of knowledge of the other parties for learning or enhancing its own policies. We refer to this learning mechanism as policy transfer. The design of a policy transfer framework has challenges, including policy conflicts and privacy issues. Policy conflicts typically arise because of differences in the obligations of the parties, whereas privacy issues result because of data sharing constraints for sensitive data. Hence, the policy transfer framework should be able to tackle such challenges by considering minimal sharing of data and support policy adaptation to address conflict. In the paper we propose a framework that aims at addressing such challenges. We introduce a formal definition of the policy transfer problem for attribute-based policies. We then introduce the transfer methodology that consists of three sequential steps. Finally we report experimental results.
Cross-metathesis of Allenes. Mechanistic Analysis and Identification of a Ru-CAAC as the Most Effective Catalyst (2021)

The first examples of cross-metathesis between two different allenes is disclosed. First- and second-generation Ru complexes were found to be ineffective, at most affording only oligomeric products. The exception was a first-generation complex bearing a bidentate phenyl isopropoxy ligand (i.e., PCy<sub>3</sub> is not released upon initiation), reactions with which afforded a 1,3-disubstituted allenyl boronate in 22% yield. On the basis of mechanistic studies designed to gain deeper understanding of the reasons for the ineffectiveness of different Ru catalysts, it was discovered that phosphine-free Ru-CAAC complexes have the steric and electronic attributes to be highly effective. The results of these investigations pave the way for development of additional olefin metathesis reactions that generate allenes.
Cucurbita ficifolia Fruit Extract Induces Tp53/Caspase‐Mediated Apoptosis in MCF‐7 Breast Cancer Cells (2020)

The second most biggest cancer worldwide is breast cancer. There is an increasing need for safer, effective, and affordable drug candidates from natural sources to treat breast cancer. In the present investigation, the anticancer effect of <i>Cucurbita ficifolia</i> Bouché (<i>C</i>. <i>ficifolia</i>) fruit extract was tested on the human breast cancer cells such as MCF-7. The cells were exposed with different doses of <i>C</i>. <i>ficifolia</i>, for the assessment of IC<sub>50</sub> concentrations on the MCF-7 cell lines for 24 hs. The effect of <i>C</i>. <i>ficifolia</i> fruit extract on morphological and apoptotic changes were evaluated by specific fluorescence staining techniques and real-time PCR in a time-dependent manner for 24 hs and 48 hs. The IC<sub>50</sub> value for <i>C</i>. <i>ficifolia</i> fruit extract was found to be 90 <i>μ</i>g/mL. Morphological alteration and apoptotic distinctiveness aspect like chromatin condensation and nuclear fragmentation were noticed in <i>C. ficifolia</i> extract exposed breast cancer cells. Further, we observed that <i>C</i>. <i>ficifolia</i> extract-induced programmed cell death in the MCF-7 cells were mediated with the elevated expression of the tumor suppressor gene such as p53 and apoptotic markers such as caspase-8, caspase-9, caspase-3, fatty acid synthase (FAS), Fas-associated protein with death domain (FADD), Bcl-2 homologous antagonist/killer (BAK), and Bcl-2-associated X protein (BAX). These observations established that <i>C</i>. <i>ficifolia</i> significantly concealed the cell division and provoked p53/caspase-mediated programmed cell death. Further, we noticed that this cell death in MCF-7 cells is concentration and time dependent. As evaluated through the comet assay, <i>C</i>. <i>ficifolia</i> induced DNA damage; further upon increasing the duration of the treatment, the DNA damage was higher than before. Thus, our study concludes that <i>C</i>. <i>ficifolia</i> could serve as an effective anticancer agent through vital gene modulation.
Double-Walled Carbon Nanotubes Under Hydrostatic Pressure: Raman Experiments and Simulations (2007)

We have used Raman spectroscopy to study the behavior of double-walled carbon nanotubes (DWNT) under hydrostatic pressure. We find that the rate of change of the tangential mode frequency with pressure is higher for the sample with traces of polymer compared to the pristine sample. We have performed classical molecular dynamics simulations to study the collapse of single (SWNT) and double-walled carbon nanotube bundles under hydrostatic pressure. The collapse pressure (pc) was found to vary as 1/R3, where R is the SWNT radius or the DWNT effective radius. The bundles showed approximately 30% hysteresis and the hexagonally close packed lattice was completely restored on decompression. The pc of a DWNT bundle was found to be close to the sum of its values for the inner and the outer tubes considered separately as SWNT bundles, demonstrating that the inner tube supports the outer tube and that the effective bending stiffness of DWNT, D(DWNT) - 2D(SWNT).
Digital compensation of the optical line: Pre-distortion Tx &#x00026; coherent Rx (2008)

Precompensation and coherent postcompensation mitigate many of the depredations of the optical line. Fiber loss, and consequential amplifier noise, remains the major issue.
259-OR: The Effect of Impaired Glucagon Suppression on Endogenous Glucose Production Is Independent of Insulin Action (2021)

Type 2 diabetes is a disease characterized by impaired insulin secretion and defective glucagon suppression in the postprandial period. We examined the effect of impaired glucagon suppression on glucose concentrations and Endogenous Glucose Production (EGP) at different degrees of insulin secretory impairment. The contribution of anthropometric characteristics, peripheral, and hepatic, insulin action to this variability was also examined. To do so, we studied 54 nondiabetic subjects on two occasions in which endogenous hormone secretion was inhibited by somatostatin, with glucagon infused at a rate of 0.65 ng/kg/min, at 0 min to prevent a fall in glucagon (non-suppressed day) or at 120 min to create a transient fall in glucagon (suppressed day). Subjects received glucose (labeled with [3-3H]-glucose) infused to mimic the systemic appearance of 50g oral glucose. Insulin was infused to mimic a prandial insulin response in 18 subjects, another 18 received 80% of the dose and the remaining 18 received 60%. EGP was measured using the tracer-dilution technique. Decreased prandial insulin resulted in greater % increase in peak glucose (56±5 vs. 44±6 vs. 31±6 %, p=0.01 - 0.6, 0.8 and 1.0 dose respectively) but not in integrated (area above basal - AAB) glucose (39±4 vs. 43±9 vs. 36±9 %, p=0.82) caused by non-suppressed glucagon. The % change in integrated EGP was unaffected by insulin dose (51±20 vs. 27±19 vs. 22±18 %, p=0.53). Multivariate regression analysis, adjusted for age, sex, weight and insulin dose, did not show a relationship (R2=0.04, p=0.21) between the EGP response to impaired suppression of glucagon and insulin action as measured at the time of screening by oral glucose tolerance. A similar analysis for hepatic insulin action did not show a relationship (R2=0.06, p=0.06) with the EGP response to non-suppressed glucagon. These data indicate that the change in EGP produced by impaired glucagon suppression is independent of anthropometric characteristics and insulin action. Disclosure M. C. Laurenti: None. A. M. Egan: None. D. Schembri wismayer: None. C. Cobelli: None. C. Dalla man: Research Support; Self; Becton, Dickinson and Company, Sanofi-Aventis Deutschland GmbH. A. Vella: Research Support; Self; Novo Nordisk. Funding National Institutes of Health (DK116231, DK78646)
Supplementary Table 2 from Pacritinib Combined with Sirolimus and Low-Dose Tacrolimus for GVHD Prevention after Allogeneic Hematopoietic Cell Transplantation: Preclinical and Phase I Trial Results (2023)

&lt;p&gt;The table shows donor/recipient CMV serostatus.&lt;/p&gt;
Sediment i Laxsjön : Halter av miljöfarliga ämnen (2012)

Denna rapport presenterar resultat från den sedimentprovtagning som gjordes den 14-15 juni 2011 för att ta fram underlag för kemisk och ekologisk statusklassning. Provtagningen var även ett led i att ta fram och utvärdera en kommande vägledning: Operativ övervakning av miljögifter – Praktisk vägledning.Ytsedimenten i Laxsjön har undersökts på fem lokaler och främst två av dessa bedöms vara påverkade av miljöfarliga ämnen. Den ena lokalen (LX2), visade på tydligt förhöjda halter av framförallt DEHP, koppar och klorerade substanser: dioxiner, klorparaffiner (C14-16), PCP och PCB. I ett nationellt perspektiv anses halterna av DEHP, koppar och dioxiner höga men uppföljande studier behöver göras för att undersöka historiska trender och biotillgänglighet av dessa ämnen. I den andra påverkade lokalen (LX4) påträffades något förhöjda halter av PCBer, vissa PAHer och TBT. Resultaten utvärderades också med avseende på risk för effekter och en preliminär bedömning av status enligt vattenförvaltningsförordningen. Halten av DEHP i LX2 indikerar risk för effekter (sänkt status) på sedimentlevande organismer. Halterna av TBT, och nickel överskrider de nivåer som kan tänkas ge effekter, men detta gäller på samtliga lokaler och troligen även för ett stort antal platser i Sverige.Som kompletterande studier till de kemiska analyserna gjordes även cellbaserade tester. In vitro testet "DR CALUX" bekräftade de kemiska analysresultaten för dioxiner, dibenzofuraner och plana PCBer efter framräkning av TCDDekv, dvs. att det främst är LX2 som är belastad av den här typen av stabila substanser. Dioxinlika effekter kan dock även uppstå till följd av exponering för ett stort antal PAHer varav flera inte normalt ingår i den kemiska analysen. Denna respons kan analyseras med PAH CALUX och detta test gav utslag främst i LX2 och LX4. Responsen i ER CALUX är särskilt förhöjd i LX 4 vilket inte är förvånande med tanke på närvaron av ett reningsverk och att testet ger utslag på östrogena ämnen. Även de andra in vitro testerna, PPAR gamma, Anti-AR och ER CALUX indikerar att det är störst respons i LX2 och LX4.
Brillouin scattering studies of polymeric nanostructures * (2004)

Abstract For a range of applications, polymers are now being patterned into nanometer‐sized features. In these applications, the robust mechanical properties of the nanostructures are critical for performance and stability. Brillouin light scattering is presented as a nondestructive, noncontact tool used to quantify the elastic constants in such nanostructures. We demonstrate this through a series of thin films and parallel ridges and spacings (gratings) with ridge widths ranging from 180 to 80 nm. For the set of films and structures presented here, the room‐temperature elastic moduli did not change with decreasing film thickness or grating ridge width, and this implied that one‐dimensional and two‐dimensional confinement‐induced changes of the mechanical properties were not significant down to feature sizes of 80 nm. Additionally, Brillouin spectra of submicrometer gratings revealed new modes not present in the spectra of thin films. The origin of these new modes remains unclear. © 2004 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 42: 1106–1113, 2004
Acat1 gene KO restores TGN cholesterol deficiency in mutant NPC1 cells and expands mutant Npc1 mouse lifespan (2020)

Abstract Niemann-Pick type C (NPC) is a neurological disorder with no cure. NPC proteins deliver cholesterol from endosomes to other compartments including trans-Golgi network (TGN) and endoplasmic reticulum (ER). Acyl-coenzyme A:cholesterol acyltransferase 1 (ACAT1) is a resident ER enzyme that converts cholesterol to cholesteryl esters for storage. Here, we report the surprising finding that in a mutant Npc1 mice, Acat1 -deficiency delayed the onset of weight loss and declining motor skill, prolonged lifespan, delayed Purkinje neuron death, and improved hepatosplenic pathology. Furthermore, syntaxin 6, a cholesterol-binding t-SNARE normally localized to TGN, is mislocalized in mutant NPC cells. However, upon ACAT1 inhibition this mislocalization is corrected, and increase the level of a few proteins further downstream. Our results imply that ACAT1 inhibition diverts a cholesterol storage pool in a way that replenished the low cholesterol level in NPC-deficient TGN. Taking together, we identify ACAT1 inhibition as a potential therapeutic target for NPC treatment.