gebhart/patrepeval huggingface dataset:
Measles Virus Glycoprotein-Pseudotyped Lentiviral Vector-Mediated Gene Transfer into Quiescent Lymphocytes Requires Binding to both SLAM and CD46 Entry Receptors (2011)

Gene transfer into quiescent T and B cells is of importance for gene therapy and immunotherapy approaches to correct hematopoietic disorders. Previously, we generated lentiviral vectors (LVs) pseudotyped with the Edmonston measles virus (MV) hemagglutinin and fusion glycoproteins (Hgps and Fgps) (H/F-LVs), which, for the first time, allowed efficient transduction of quiescent human B and T cells. These target cells express both MV entry receptors used by the vaccinal Edmonston strain, CD46 and signaling lymphocyte activation molecule (SLAM). Interestingly, LVs pseudotyped with an MV Hgp, blind for the CD46 binding site, were completely inefficient for resting-lymphocyte transduction. Similarly, SLAM-blind H mutants that recognize only CD46 as the entry receptor did not allow stable LV transduction of resting T cells. The CD46-tropic LVs accomplished vector-cell binding, fusion, entry, and reverse transcription at levels similar to those achieved by the H/F-LVs, but efficient proviral integration did not occur. Our results indicate that both CD46 and SLAM binding sites need to be present in cis in the Hgp to allow successful stable transduction of quiescent lymphocytes. Moreover, the entry mechanism utilized appears to be crucial: efficient transduction was observed only when CD46 and SLAM were correctly engaged and an entry mechanism that strongly resembles macropinocytosis was triggered. Taken together, our results suggest that although vector entry can occur through the CD46 receptor, SLAM binding and subsequent signaling are also required for efficient LV transduction of quiescent lymphocytes to occur.
ChemInform Abstract: Heterocyclic Structures Useful in Medicinal Chemistry: The Case of Pyrazino(2,3‐c)(1,2,6)thiadiazine 2,2‐Dioxide (1997)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Abstract B037: Potential next-generation androgen receptor-targeted therapeutic for enzalutamide-resistant prostate cancer; In vivo characterization in immune-compromised SRG rats (2019)

Abstract Current treatment options for advanced castration-resistant prostate cancers (CRPC) are effective for a brief period before becoming refractory. It is important to use relevant in vivo models for candidate selection in the development of next-generation mechanistically-distinct drugs to treat castration- and drug- resistant prostate cancers. Here, we describe a series of AR pan-antagonists (selective AR degraders (SARDs)) that degrade the AR and AR splice variants. SARDs inhibit the wild-type and LBD mutant ARs comparably and inhibit the in vitro proliferation and in vivo growth of enzalutamide-sensitive and resistant prostate cancer xenografts. Xenograft studies conducted in immune-compromised SRG rats (Sprague Dawley Rag2-/- Il2rg -/-; Hera Biolabs) with three lead SARDs demonstrated regression of enzalutamide-sensitive and -resistant VCaP tumors both in castrated and in intact rats. SRG rats provide the benefit of abundant blood samples for weekly evaluation of rising PSA, which also indicated that the SARDs inhibit the rising PSA, while enzalutamide was inactive in enzalutamide-resistant model. Drug metabolism and pharmacokinetic (DMPK) studies, also conducted with SRG and wild-type rats and in combination with efficacy, indicate that the molecules possess all the necessary drug-like properties. The molecules exhibit a broad safety margin with no cross-reactivity with G-Protein Coupled Receptor, kinase, and nuclear receptor family members. Collectively, the SARDs exhibit the properties necessary for a next-generation prostate cancer drug and that use of SRG rats facilitated thorough characterization of their in vivo properties. Citation Format: Suriyan Udhayasuriyan Ponnusamy, Fallon K Noto, Bisoye Towobola Adedeji, Yali He, Dong-Jin Hwang, Sam Moody, Thirumagal Thiyagarajan, Tseten Yeshi Jamling, Duane D Miller, Ramesh Narayanan. Potential next-generation androgen receptor-targeted therapeutic for enzalutamide-resistant prostate cancer; In vivo characterization in immune-compromised SRG rats [abstract]. In: Proceedings of the AACR-NCI-EORTC International Conference on Molecular Targets and Cancer Therapeutics; 2019 Oct 26-30; Boston, MA. Philadelphia (PA): AACR; Mol Cancer Ther 2019;18(12 Suppl):Abstract nr B037. doi:10.1158/1535-7163.TARG-19-B037
λ–MIMO: Massive MIMO Via Modulo Sampling (2023)

Massive multiple-input multiple-output (M-MIMO) architecture is the workhorse of modern communication systems. Currently, two fundamental bottlenecks, namely, power consumption and receiver saturation, limit the full potential achievement of this technology. These bottlenecks are intricately linked with the analog-to-digital converter (ADC) used in each radio frequency (RF) chain. The power consumption in M-MIMO systems grows exponentially with the ADC’s bit budget while ADC saturation causes permanent loss of information. This motivates the need for a solution that can simultaneously tackle the above-mentioned bottlenecks while offering advantages over existing alternatives such as low-resolution ADCs. Taking a radically different approach to this problem, we propose <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\lambda $ </tex-math></inline-formula> –MIMO architecture which uses modulo ADCs ( <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\mathscr {M}_{\lambda} $ </tex-math></inline-formula> –ADC) instead of a conventional ADC. Our work is inspired by the Unlimited Sampling Framework. <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\mathscr {M}_{\lambda} $ </tex-math></inline-formula> –ADC in the RF chain folds high dynamic range signals into low dynamic range modulo samples, thus alleviating the ADC saturation problem. At the same time, digitization of modulo signal results in high resolution quantization. In the novel <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\lambda $ </tex-math></inline-formula> –MIMO context, we discuss baseband signal reconstruction, detection and uplink achievable sum-rate performance. The key takeaways of our work include, (a) leveraging higher signal-to-quantization noise ratio (SQNR), (b) detection and average uplink sum-rate performances comparable to a conventional, infinite-resolution ADC when using a 1–2 bit <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\mathscr {M}_{\lambda} $ </tex-math></inline-formula> –ADC. This enables higher order modulation schemes e.g., 1024 QAM that seemed previously impossible, (c) superior trade-off between energy efficiency and bit budget, thus resulting in higher power efficiency. Numerical simulations and modulo ADC based hardware experiments corroborate our theory and reinforce the clear benefits of <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\lambda $ </tex-math></inline-formula> –MIMO approach.
Target engagement of the first-in-class CXCR7 antagonist ACT-1004-1239 following multiple-dose administration in mice and humans (2021)

Antagonism of the chemokine receptor CXCR7 has shown promising effects in diverse disease areas through modulation of its ligands, CXCL11 and CXCL12. Preclinical data of the first-in-class CXCR7 antagonist, ACT-1004-1239, showed efficacy in animal models of multiple sclerosis and acute lung injury. In healthy humans, single-dose administration of ACT-1004-1239 revealed a favorable clinical profile. Here, we report the target engagement of ACT-1004-1239 in healthy mice and humans after multiple doses using CXCL11 and CXCL12 as biomarkers. In addition, safety/tolerability, concentration-QTc relationship, and pharmacokinetics (PK) were assessed in a randomized, double-blind, placebo-controlled Phase 1 clinical study. Multiple-dose ACT-1004-1239 dose-dependently increased CXCL12 plasma concentration across the investigated dose range in mice and humans (mice: 1-100 mg/kg b.i.d.; humans: 30-200 mg o.d.) when compared to vehicle/placebo demonstrating target engagement. Mouse and human PK/PD models predicted that CXCL12 concentration approached a plateau within these dose ranges. In humans, ACT-1004-1239 was rapidly absorbed (t<sub>max</sub>: 1.75-3.01 h) and the terminal t<sub>1/2</sub> was approximately 19 h. Steady-state conditions were reached by Day 3 with an accumulation index of 1.2. Female subjects had overall higher exposure compared to males. Multiple-dose ACT-1004-1239 was well tolerated up to 200 mg once daily in humans. There was no evidence of ACT-1004-1239-mediated QTc interval prolongation. Overall, multiple oral doses of ACT-1004-1239 showed target engagement with CXCR7 in healthy mice and humans, therefore, assessment of CXCL12 as translational tool for further investigations in patients is warranted. Favorable safety/tolerability and PK profiles allow for further clinical development.
ChemInform Abstract: Synthesis of 2,2‐Dimethyl‐4‐cyclopentene‐1,3‐dione and 5,5‐Dimethyl‐4‐ hydroxy‐2‐cyclopenten‐1‐one. (1997)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Inducible cAMP Early Repressor Inhibits Growth of Vascular Smooth Muscle Cell (2007)

These results suggest that ICER induces apoptosis and inhibits proliferation of VSMCs, and plays a critical role in beraprost-mediated suppression of VSMC proliferation. ICER may be an important endogenous inhibitor of vascular proliferation.
Detection of foetal single gene mutations using only maternal blood samples (2021)

Non-Invasive Prenatal Testings (NIPT) for chromosomal aneuploidy are widely applied but not yet for monogenic diseases. In this study, we have developed new analysis algorithms for detecting foetal single gene mutations that are linked to a mendelian disease by sequencing maternal blood samples. The proposed algorithm used two approaches to determine the foetal mutation status. If the mutation type is a duplication, we use the allele frequency of the heterozygous site, and if the mutation is a deletion, we use the ratio of the relative read depth of cell-free DNA to the parent genomic DNA. The algorithms were applied to real data consisting of four pairs of sequencing results generated using peripheral blood samples from two pregnant women. Both sample providers have their first child with Duchene Muscular Dystrophy (DMD) disease, a typical X-linked recessive disorder. Sequences were generated using massively parallel sequencing technologies with a targeted sequencing approach.
Characterization of Sidewall and Planar Surfaces of Electroformed LIGA Parts (2000)

The nature of surfaces and the way they interact with each other during sliding contact can have a direct bearing on the performance of a microelectromechanical (MEMS) device. Therefore, a study was undertaken to characterize the surfaces of LIGA fabricated Ni and Cu components. Sidewall and planar surfaces were examined by scanning electron microscopy (SEM) and atomic force microscopy (AFM). Surface roughness was quantified using the AFM. Post-processing (e.g. lapping, removal of polymer film) can profoundly influence the morphology of LIGA components. Edge rounding and smearing of ductile materials during lapping can result in undesirable sidewall morphologies. By judicious selection of AFM scan sizes, the native roughness ({approximately}10 nm RMS) can be distinguished from that arising due to post processing, e.g. scratches, debris, polymer films. While certain processing effects on morphology such as those due to lapping or release etch can be controlled, the true side wall morphology appears to be governed by the morphology of the polymer mold or by the electroforming process itself, and may be much less amenable to modification.
Transcranial magnetic stimulation of the brain: What is stimulated? – A consensus and critical position paper (2022)

Transcranial (electro)magnetic stimulation (TMS) is currently the method of choice to non-invasively induce neural activity in the human brain. A single transcranial stimulus induces a time-varying electric field in the brain that may evoke action potentials in cortical neurons. The spatial relationship between the locally induced electric field and the stimulated neurons determines axonal depolarization. The induced electric field is influenced by the conductive properties of the tissue compartments and is strongest in the superficial parts of the targeted cortical gyri and underlying white matter. TMS likely targets axons of both excitatory and inhibitory neurons. The propensity of individual axons to fire an action potential in response to TMS depends on their geometry, myelination and spatial relation to the imposed electric field and the physiological state of the neuron. The latter is determined by its transsynaptic dendritic and somatic inputs, intrinsic membrane potential and firing rate. Modeling work suggests that the primary target of TMS is axonal terminals in the crown top and lip regions of cortical gyri. The induced electric field may additionally excite bends of myelinated axons in the juxtacortical white matter below the gyral crown. Neuronal excitation spreads ortho- and antidromically along the stimulated axons and causes secondary excitation of connected neuronal populations within local intracortical microcircuits in the target area. Axonal and transsynaptic spread of excitation also occurs along cortico-cortical and cortico-subcortical connections, impacting on neuronal activity in the targeted network. Both local and remote neural excitation depend critically on the functional state of the stimulated target area and network. TMS also causes substantial direct co-stimulation of the peripheral nervous system. Peripheral co-excitation propagates centrally in auditory and somatosensory networks, but also produces brain responses in other networks subserving multisensory integration, orienting or arousal. The complexity of the response to TMS warrants cautious interpretation of its physiological and behavioural consequences, and a deeper understanding of the mechanistic underpinnings of TMS will be critical for advancing it as a scientific and therapeutic tool.
Silicon nanoparticle-ZnS nanophosphors for ultraviolet-based white light emitting diode (2012)

Present red phosphor converters provide spectra dominated by sharp lines and suffer from availability and stability issues which are not ideal for color mixing in display or solid state lighting applications. We examine the use of mono dispersed 3 nm silicon nanoparticles, with inhomogeneously broadened red luminescence as an effective substitute for red phosphors. We tested a 3-phase hybrid nanophosphor consisting of ZnS:Ag, ZnS:Cu,Au,Al, and nanoparticles. Correlated color temperature is examined under UV and LED pumping in the range 254, 365–400 nm. The temperature is found reasonably flat for the longer wavelengths and drops for the shorter wavelengths while the color rendering index increases. The photo stability of the phosphors relative to the silicon nanoparticles is recorded. The variation in the temperature is analyzed in terms of the strength of inter-band–gap transition and continuum band to band transitions.
A large retrospective analysis of CDK 4/6 inhibitor retreatment in ER+ metastatic breast cancer (MBC). (2019)

1053 Background: Sequential retreatment with endocrine therapy (ET) has been the clinical paradigm for ER+ MBC due to persistent dependence on hormone signaling. Recently CDK4/6i + ET have improved PFS and are routinely utilized in the first/second- line setting. Whether this paradigm of sequential retreatment holds for CDK 4/6i is unknown. To evaluate the potential benefit of CDK4/6i re-treatment we conducted this retrospective analysis. Methods: We identified ER+/HER2- MBC pts treated with ≥ 2 lines of CDK4/6i at our institution between 2015-2018. We categorized pts based on reason for discontinuation of their first CDK4/6i: cohort 1 – switch to alternate CDK4/6i due to toxicity; cohort 2 – retreatment with same CDK4/6i beyond progression with change of ET and cohort 3- switch to alternate CDK4/6i as monotherapy or with same or another ET. We analyzed pt demographics, imaging reports and time to subsequent therapy (TTST) for every CDK4/6i line for each cohort. If a pt received &gt; 2 lines of CDK4/6i, then that pt was evaluated for every CDK4/6i exposure. Results: 135 pts received ≥ 2 lines of CDK4/6i treatment (Tx). Cohorts 1, 2 and 3 had 23, 43 pts and 84 pts respectively. In Cohort 2, 95% of pts received 2 subsequent CDK 4/6i + ET Tx; 56% had the second CDK4/6i in second-line met setting. TTST1 (1st CDK4/6i Tx) was 9.6m (95% CI 4.9 - 11 m), TTST2 (second CDK4/6i Tx) was 4.5m (95% CI 3.3 – 7.6 m) and 35% had TTST2 ≥ 24 weeks. For Cohort 3, 48% were retreated with a different CDK 4/6i in ≥ fifth-line. 51% received 2 subsequent CDK 4/6i Tx with 18% in second-line met setting. TTST1 was 9.6 m (95% CI 5.9 – 12 m), TTST2 was 4.4 m (95% CI 3.8 – 5.9 m) and 29% had TTST2 ≥ 24 weeks. In cohort 3, 29% (n=24) pts had PD as best response at the time of first CDK4/6i exposure but 29% (7/24) achieved a radiologic response to their second CDK4/6i Tx. Pts had tumor sequencing using MSK-IMPACT which will be correlated with TTST. Conclusions: This large single institution retrospective analysis suggests that retreatment with a CDK4/6i regimen should be evaluated in prospective trials. Additionally, despite PD as best response with the first CDK4/6i (palbociclib/ribociclib) regimen, a subset of pts had radiologic response to a subsequent abemaciclib-containing regimen, which is an hypothesis generating observation.
Production of four Neurospora crassa lytic polysaccharide monooxygenases in Pichia pastoris monitored by a fluorimetric assay (2012)

P. pastoris is a high performing expression host for N. crassa PMOs. The pmo genes under control of the native signal sequence are correctly processed and active. The novel CDH-based enzyme assay allows fast determination of PMO activity in fermentation samples and is robust against interfering matrix components.
Clinical Pharmacokinetics of Controlled-Release Oxycodone in Renal Impairment (1996)

Clinical Pharmacology & Therapeutics (1996) 59, 130–130; doi: 10.1038/sj.clpt.1996.18
Studies Toward Diazonamide A: Initial Synthetic Forays Directed Toward the Originally Proposed Structure. (2004)

Abstract For Abstract see ChemInform Abstract in Full Text.
Improving Robustness of Codebook-Based Noise Estimation Approaches With Delta Codebooks (2011)

We present a new codebook-based speech enhancement approach which is able to increase robustness of conventional codebook-based approaches against model mismatch and unknown noise types. This is achieved by training only the difference between the actual noise and a robust estimate (e.g., obtained by minimum statistics or recursive minimum tracking) in the cepstral domain instead of the noise itself. The noise codebook is then generated by shifting the so obtained delta-codebook by the cepstral representation of a robust noise estimate. We use the recursive minimum tracking approach as robust estimate. It is thus guaranteed that the robust estimate is also a valid estimate of the codebook-based algorithm. Consequently, the codebook-based algorithm inherits the robustness from the recursive minimum tracking approach. Objective and subjective experiments show that the proposed method yields a consistent quality improvement over the basic codebook-based approach and recursive minimum tracking.
La superficie SI(100)-2x1 como dienófilo en una reacción tipo DIELS-ALDER (1999)

Se estudió la adsorción de 1,3-butadieno y de 2,3-dimetil-1,3-butadieno sobre una superficie monocristalina de Si(100)-2x1 bajo condiciones de máximo vacío (ultra-high vacuum, UHV) observándose que, a temperatura ambiente, cada dieno reacciona mediante una cicloadición [4+2] sobre el dímero Si2 de esta superficie. El producto de esta reacción ha sido comparado con el aducto formado en una reacción Diels-Alder, con el dímero superficial Si2 actuando como dienófilo. Esta reacción, a la que llamaremos del tipo Diels-Alder, ha sido estudiada bajo distintas condiciones de temperatura. El aducto Diels-Aldcr fue sometido luego a reacciones térmicas. La formación de este aducto fue detectada por espectroscopia infraroja de reflexión múltiple interna (multiple internal reflection infrared spectroscopy, MIR-IRS), espectrometría de desorción térmica (thermal desorption spectrometry), y confirmada luego al estudiar la estructura fina cerca al límite de ionización por absorción de rayos X (near-edge X-ray absorption fine structure- NEXAFS) de la especie adsorbida. En este último estudio, para 2,3-dimetil-1,3-butadieno, también se determinó el ángulo (41") entre el plano de la superficie Si( lOO) y el plano que contiene los orbitales [pi] del aducto formado. Por otro lado, al repetir la reacción tipo Diels-Alder sobre la superficie enfriada a temperaturas criogénicas (100 K), se observa más bien una adsorción física sobre la superficie, y no la reacción detectada a temperatura ambiente. Estos resultados de dependencia térmica de la reacción sugieren que hay una barrera de activación para la quimisorción a baja temperatura. Esta reacción tipo Diels-Alder no es reversible sobre la superficie Si(100)-2x1 pues, al activar térmicamente la superficie, no ocurre la desorción molecular del dieno precursor en fase gas, sino que el a dueto se descompone, formando carbono e hidrógeno adsorbidos sobre la superficie.
High Speed Wireless Networking for 60GHz (2000)

This thesis examines the problem of providing high data-rate wireless connectivity to users in indoor environments. The goal is to be able to reach Gbps/user rates even when there are multiple users present. The technology that we study is to use the 60 GHz spectrum whose special propagation properties make it ideally suited to this task. The approaches developed include using multiple spatially distributed smart antennas in a room or multiple co-located antennas to provide coverage where needed and when needed. All the antennas are connected to a single access point which allows us to dynamically change spectrum and link allocation among the users (as they move or as their needs change). The innovations in this work include the exploitation of the special properties of 60 GHz and the corresponding design of algorithms for efficient spectrum allocation. We use detailed simulations to demonstrate that very high data rates are indeed achievable.
Optical noise and dispersion monitoring with SOA-based optical 2R regenerator (2004)

Optical performance monitoring is demonstrated using the nonlinear response of a semiconductor optical amplifier. The monitoring device is derived from a recently proposed all-optical wavelength converter for 10- and 40-Gb/s return-to-zero (RZ) modulated signals. We show experimental results for both optical signal-to-noise ratio monitoring and dispersion monitoring on 40-Gb/s RZ signals and Q factor correlation on a 10-Gb/s RZ signal. The performance monitor signal correlates with the Q factor and can provide an unambiguous signal quality measure with potential application to quality of service and fault management.