gebhart/patrepeval huggingface dataset:
Extract of Perilla frutescens Enriched for Rosmarinic Acid, a Polyphenolic Phytochemical, Inhibits Seasonal Allergic Rhinoconjunctivitis in Humans (2004)

Extract of Perilla frutescens enriched for rosmarinic acid, a polyphenolic phytochemical, suppresses allergic immunoglobulin responses and inflammation caused by polymorphonuclear leukocytes (PMNL) in mice. However, few placebo-controlled clinical trials have examined the efficacy and safety of polyphenolic phytochemicals for treatment of allergic inflammatory diseases in humans. The present study determined whether oral supplementation with rosmarinic acid is an effective intervention for patients with seasonal allergic rhinoconjunctivitis (SAR). In this 21-day, randomized, double-blind, age-matched, placebo-controlled parallel group study, patients with mild SAR were treated daily with extract of Perilla frutescens enriched for rosmarinic acid (200 mg [n=10] or 50 mg [n=9]) or placebo (n=10). Patients recorded symptoms daily in a diary. Profiles of infiltrating cells and concentrations of eotaxin, IL-1beta, IL-8, and histamine were measured in nasal lavage fluid. Serum IgE concentrations and routine blood tests were also examined. As compared with placebo supplementation, supplementation with extract of Perilla frutescens enriched for rosmarinic acid resulted in a significant increase in responder rates for itchy nose, watery eyes, itchy eyes, and total symptoms (P<0.05). Active treatment significantly decreased the numbers of neutrophils and eosinophils in nasal lavage fluid (P<0.05 vs. placebo). Patients reported no adverse events, and no significant abnormalities were detected in routine blood tests. In conclusion, extract of Perilla frutescens enriched for rosmarinic acid can be an effective intervention for mild SAR at least partly through inhibition of PMNL infiltration into the nostrils. Use of this alternative treatment for SAR might reduce treatment costs for allergic diseases.
Polymer Surface Transport Is a Combination of in-Plane Diffusion and Desorption-Mediated Flights (2016)

Previous studies of polymer motion at solid/liquid interfaces described the transport in the context of a continuous time random walk (CTRW) process, in which diffusion switches between desorption-mediated "flights" (i.e., hopping) and surface-adsorbed waiting-time intervals. However, it has been unclear whether the waiting times represented periods of complete immobility or times during which molecules engaged in a different (e.g., slower or confined) mode of interfacial transport. Here we designed high-throughput, single-molecule tracking measurements to address this question. Specifically, we studied polymer dynamics on either chemically homogeneous or nanopatterned surfaces (hexagonal diblock copolymer films) with chemically distinct domains, where polymers were essentially excluded from the low-affinity domains, eliminating the possibility of significant continuous diffusion in the absence of desorption-mediated flights. Indeed, the step-size distributions on homogeneous surfaces exhibited an additional diffusive mode that was missing on the chemically heterogeneous nanopatterned surfaces, confirming the presence of a slow continuous mode due to 2D in-plane diffusion. Kinetic Monte Carlo simulations were performed to test this model and, with the theoretical in-plane diffusion coefficient of <i>D</i><sub>2D</sub> = 0.20 μm<sup>2</sup>/s, we found a good agreement between simulations and experimental data on both chemically homogeneous and nanopatterned surfaces.
Data from Mitochondrial Cytochrome B Gene Mutation Promotes Tumor Growth in Bladder Cancer (2023)

&lt;div&gt;Abstract&lt;p&gt;Mitochondria-encoded Cytochrome B (&lt;i&gt;CYTB&lt;/i&gt;) gene mutations were reported in different cancers, but the effect of these mutations on cellular metabolism and growth is unknown. In a murine xenograft and human model of bladder cancer, we show the functional effect of overexpression of a 21-bp deletion mutation (&lt;i&gt;mt&lt;/i&gt;) of &lt;i&gt;CYTB&lt;/i&gt;. Overexpression of &lt;i&gt;mtCYTB&lt;/i&gt; generated increased reactive oxygen species (ROS) accompanied by increased oxygen consumption and lactate production. &lt;i&gt;MtCYTB&lt;/i&gt; overexpression induced significant tumor growth &lt;i&gt;in vitro&lt;/i&gt; and &lt;i&gt;in vivo&lt;/i&gt; by triggering rapid cell cycle progression through up-regulation of the nuclear factor-κB2 signaling pathway. Tumor-generated ROS induced &lt;i&gt;in vitro&lt;/i&gt; lysis of normal splenocytes. Thus, we present physiologic and functional evidence for the role of a bonafide mitochondrial gene mutation in cancer. [Cancer Res 2008;68(3):700–06]&lt;/p&gt;&lt;/div&gt;
Decreased pCO2 accumulation by eliminating bicarbonate addition to high cell‐density cultures (2006)

High-density perfusion cultivation of mammalian cells can result in elevated bioreactor CO(2) partial pressure (pCO(2)), a condition that can negatively influence growth, metabolism, productivity, and protein glycosylation. For BHK cells in a perfusion culture at 20 x 10(6) cells/mL, the bioreactor pCO(2) exceeded 225 mm Hg with approximate contributions of 25% from cellular respiration, 35% from medium NaHCO(3), and 40% from NaHCO(3) added for pH control. Recognizing the limitations to the practicality of gas sparging for CO(2) removal in perfusion systems, a strategy based on CO(2) reduction at the source was investigated. The NaHCO(3) in the medium was replaced with a MOPS-Histidine buffer, while Na(2)CO(3) replaced NaHCO(3) for pH control. These changes resulted in 63-70% pCO(2) reductions in multiple 15 L perfusion bioreactors, and were reproducible at the manufacturing-scale. Bioreactor pCO(2) values after these modifications were in the 68-85 mm Hg range, pCO(2) reductions consistent with those theoretically expected. Low bioreactor pCO(2) was accompanied by both 68-123% increased growth rates and 58-92% increased specific productivity. Bioreactor pCO(2) reduction and the resulting positive implications for cell growth and productivity were brought about by process changes that were readily implemented and robust. This philosophy of pCO(2) reduction at the source through medium and base modification should be readily applicable to large-scale fed-batch cultivation of mammalian cells.
Reconstitution of Src-dependent Phospholipase Cγ Phosphorylation and Transient Calcium Release by Using Membrane Rafts and Cell-free Extracts from Xenopus Eggs (2003)

We reported previously that egg membrane rafts serve as a subcellular microdomain for sperm-dependent tyrosine kinase signaling in Xenopus fertilization. Moreover, we demonstrated that raft-associated Src tyrosine kinase was activated by sperm in vitro. Here we show that egg rafts incubated with sperm or hydrogen peroxide (H2O2) can promote Src-dependent phosphorylation of phospholipase Cgamma (PLCgamma) and transient calcium release in the extracts of unfertilized Xenopus eggs. In vivo egg activation by sperm or H2O2 also promotes tyrosine phosphorylation and raft-translocalization of PLCgamma. Immunodepletion of PLCgamma from the egg extracts inhibits the raft-dependent calcium release. Rafts prepared from H2O2-activated eggs also promote Src-dependent dephosphorylation of p42 mitogen-activated protein kinase and cell cycle transition from metaphase II to interphase in egg extracts. PLCgamma phosphorylation and calcium release in egg extracts can be promoted by rafts prepared from COS-7 cells expressing the Xenopus Src gene. These results demonstrate that the signaling events elicited by fertilization in Xenopus eggs can be reconstituted in vitro. The development of such experimental platforms will allow us to dissect the molecular mechanism of sperm-dependent activation of raft-associated Src and subsequent up-regulation of PLCgamma and egg activation machinery in Xenopus eggs.
Readings in computer architecture (2000)

Chapter 1 - Classic Machines: Technology, Implementation, and Economics Chapter 2 - Methods Chapter 3 - Instruction Sets Chapter 4 - Instruction Level Parallelism (ILP) Chapter 5 - Dataflow and Multithreading Chapter 6 - Memory Systems Chapter 7 - I/O: Storage Systems, Networks, and Graphics Chapter 8 - Single-Instruction Multiple Data (SIMD) Parallelism Chapter 9 - Multiprocessors and Multicomputers Chapter 10 - Recent Implementations and Future Prospects
Adaptive rate-dependent feedforward controller for hysteretic piezoelectric actuator (2008)

With the increasing popularity of actuators involving smart materials like piezoelectric, control of such materials becomes important. The existence of the inherent hysteretic behavior hinders the tracking accuracy of the actuators. To make matters worse, the hysteretic behavior changes with rate. One of the suggested ways is to have a feedforward controller to linearize the relationship between the input and output. Thus, the hysteretic behavior of the actuator must be first modeled by sensing the relationship between the input voltage and output displacement. Unfortunately, the hysteretic behavior is dependent on individual actuator and also environmental conditions like temperature. In this fast moving world, time is money and it is very costly to model the hysteresis regularly. In addition, the hysteretic behavior of the actuators also changes with age. Base on the studies done on the phenomena hysteretic behavior with rate, this paper proposes an adaptive rate-dependent feedforward controller with Prandtl-Ishlinskii (PI) hysteresis operators for piezoelectric actuators. This adaptive controller is achieved by adapting the coefficients to manipulate the weights of the play operators. Actual experiments are conducted to demonstrate the effectiveness of the adaptive controller.
Exploiting predictability in click-based graphical passwords (2011)

We provide an in-depth study of the security of click-based graphical password schemes like PassPoints (Weidenbeck et al., 2005), by exploring popular points (hot-spots), and examining strategies to predict and exploit them in guessing attacks. We report on both short- and long-term user studies: one lab-controlled, involving 43 users and 17 diverse images, the other a field test of 223 user accounts. We provide empirical evidence that hot-spots do exist for many images, some more so than others. We explore the use of “human-computation” (in this context, harvesting click-points from a small set of users) to predict these hot-spots. We generate two “human-seeded” attacks based on this method: one based on a first-order Markov model, another based on an independent probability model. Within 100 guesses, our first-order Markov model-based attack finds 4% of passwords in one image's data set, and 10% of passwords in a second image's data set. Our independent model-based attack finds 20% within 2 33 guesses in one image's data set and 36% within 2 31 guesses in a second image's data set. These are all for a system whose full password space has cardinality 2 43 . We evaluate our first-order Markov model-based attack with cross-validation of the field study data, which finds an average of 7–10% of user passwords within 3 guesses. We also begin to explore some click-order pattern attacks, which we found improve on our independent model-based attacks. Our results suggest that these graphical password schemes (with parameters as originally proposed) are vulnerable to offline and online attacks, even on systems that implement conservative lock-out policies.
Nanostructured Biodegradable Polymer Networks Using Lyotropic Liquid Crystalline Templates (2007)

The physical properties, porosity, and physiological behavior of synthetic biodegradable hydrogels have been identified as highly critical design parameters in most tissue engineering materials applications. Nanotechnology may provide the means to manipulate these parameters by accessing control over the network structure of the biomaterial, providing unique property relationships that often result from nanostructured materials. In this study, a lyotropic liquid crystal (LLC) was used as a polymerization template in the formation of a photopolymerizable biodegradable PLA-b-PEG-b-PLA (PEG = poly(ethylene glycol); PLA = poly(lactic acid)) material with nanoscale lamellar morphology. Through ordering of the biodegradable monomer within the liquid crystal assembly, a 2-fold increase in maximum polymerization rate and a 30% increase in double bond conversion were realized over isotropic monomer formulations. The resulting network structure of the templated PLA-b-PEG-b-PLA material has a dramatic affect on the physical properties of the hydrogel including an 80% increase in network swelling and an approximately 230% increase in diffusivity. This increase in permeability and solvent uptake leads to rapid degradation of the lamellar templated samples, further demonstrating the influence of the LLC directed network structure on the porosity and physical properties of the biodegradable material. The ability to control the porosity, physical properties, and behavior of a biodegradable hydrogel simply by imparting LLC network structure, without changing the chemistry or biocompatibility of the polymer, could prove highly advantageous in the design of synthetic biomaterials for potential medical applications.
Starch Nanocomposites with Platelet and Fibrillar Fillers (2009)

Cast amylopectin-based nanocomposite films were prepared and characterized with focus on their structures and mechanical properties. Sodium-rich montmorillonite (MMT) clay was used as a filler toge ...
Low-complexity model predictive control of ElectroMagnetic actuators (2009)

Electromagnetically driven mechanical systems are characterized by fast non-linear dynamics that are subject to physical and control constraints. This paper describes a Model Predictive Controller (MPC) for a general ElectroMagnetic (EM) actuator that satisfies both the performance constraints and the strict requirements on the computation time. Novel aspects of the MPC design are a one-step-ahead prediction horizon and an infinity-norm artificial Lyapunov function that is employed to drive the system to a desired reference. An additional optimization variable is introduced to relax the conditions on the Lyapunov function, which is not forced to decrease monotonically. This feature improves feasibility considerably. The resulting MPC problem is transformed into a low-complexity linear program that can be solved by modern microprocessors within tenths of milliseconds. An even simpler piecewise affine explicit controller is obtained via multiparametric programming. Simulation results are reported and compared with the results achieved by existing state-of-the-art explicit MPC.
New Evidence for Genome-Wide Duplications at the Origin of Vertebrates Using an Amphioxus Gene Set and Completed Animal Genomes (2003)

The 2R hypothesis predicting two genome duplications at the origin of vertebrates is highly controversial. Studies published so far include limited sequence data from organisms close to the hypothesized genome duplications. Through the comparison of a gene catalog from amphioxus, the closest living invertebrate relative of vertebrates, to 3453 single-copy genes orthologous between Caenorhabditis elegans (C), Drosophila melanogaster (D), and Saccharomyces cerevisiae (Y), and to Ciona intestinalis ESTs, mouse, and human genes, we show with a large number of genes that the gene duplication activity is significantly higher after the separation of amphioxus and the vertebrate lineages, which we estimate at 650 million years (Myr). The majority of human orthologs of 195 CDY groups that could be dated by the molecular clock appear to be duplicated between 300 and 680 Myr with a mean at 488 million years ago (Mya). We detected 485 duplicated chromosomal segments in the human genome containing CDY orthologs, 331 of which are found duplicated in the mouse genome and within regions syntenic between human and mouse, indicating that these were generated earlier than the human-mouse split. Model based calculations of the codon substitution rate of the human genes included in these segments agree with the molecular clock duplication time-scale prediction. Our results favor at least one large duplication event at the origin of vertebrates, followed by smaller scale duplication closer to the bird-mammalian split.
A Practical Synthesis of Renin Inhibitor MK-1597 (ACT-178882) via Catalytic Enantioselective Hydrogenation and Epimerization of Piperidine Intermediate (2011)

A practical enantioselective synthesis of renin inhibitor &lt;b&gt;MK-1597&lt;/b&gt; (ACT-178882), a potential new treatment for hypertension, is described. The synthetic route provided &lt;b&gt;MK-1597&lt;/b&gt; in nine steps and 29% overall yield from commercially available &lt;i&gt;p&lt;/i&gt;-cresol (&lt;b&gt;7&lt;/b&gt;). The key features of this sequence include a catalytic asymmetric hydrogenation of a tetrasubstituted ene−ester, a highly efficient epimerization/saponification sequence of &lt;b&gt;4&lt;/b&gt; which sets both stereocenters of the molecule, and a short synthesis of amine fragment &lt;b&gt;2&lt;/b&gt;.
Speaking for Language-Impaired Children (2000)

Larry Leonard's new book, Children With Specific Language Impairment , has the potential to become the definitive text and scholarly resource for students, clinicians, and scientists concerned with these children. This six-part book (comprising 14 chapters) provides a complete, detailed, and even-handed review of the multidisciplinary research in this field.
Direct Oxidative Heck Cyclizations: Intramolecular Fujiwara–Moritani Arylations for the Synthesis of Functionalized Benzofurans and Dihydrobenzofurans (2004)

Ohne separaten Funktionalisierungsschritt gelingen Palladium(II)-katalysierte oxidative Carbocyclisierungen wie die gezeigte, die durch Nettodehydrierung hoch substituierte Benzo- und Dihydrobenzofurane liefern. Der Mechanismus ähnelt dem von Heck-Cyclisierungen; mit der Reaktion lassen sich Produkte mit quartären Kohlenstoffstereozentren in diastereomerenreiner Form erhalten.
Analyzing the scaffold immune microenvironment using flow cytometry: practices, methods and considerations for immune analysis of biomaterials (2019)

The immune system has evolved as a powerful tool for our body to combat infections, and is being engineered for new treatments in cancer and autoimmune disease. More recently, the complex role of the immune system is being recognized in tissue repair, regenerative medicine and biomaterial responses. From these combined interests, the field of immunoengineering is rapidly growing. However, bridging immunology with engineering poses numerous challenges including the biological complexity, language of immunology and accurately leveraging the powerful techniques of immunology to new applications. Elucidating the identity and function of immune cell populations responding to engineering systems will be required for continued advancement. Multi-color flow cytometry is a central technique used by immunologists for this purpose that requires careful control of variables, data acquisition, and interpretation. Here, we present methods for multi-color flow cytometry experimental design and analysis focused on characterizing the scaffold immune microenvironment in regenerative medicine research.
Surface Properties and Catalytic Activity of Ni/Al2O3–AlPO4 Catalysts (1999)

A series of Ni/Al 2 O 3 –AlPO 4 catalysts has been prepared, thereby allowing the effect of nickel content and calcination temperature on the structural and catalytic activity of such solids to be investigated. The textural parameters of all the prepared samples were determined from nitrogen adsorption studies conducted at −196°C, while thermal analysis methods (DTA, TGA and DrTGA) and X-ray diffraction techniques were used to elucidate their structures. Their surface acidities were measured by the amine titration method, while the conversion of isopropanol on the prepared catalysts was investigated using a pulse microcatalytic reactor. Significant changes in the structure, texture, surface acidity and catalytic activity of the prepared solids were observed as a result of varying their nickel content and calcination temperatures. Thus, XRD studies demonstrated the presence of nickel metal, nickel aluminate spinel, γ-alumina and tridymite phases while both the surface areas and the porous structures were found to depend on the metal content and calcination temperatures. The catalytic selectivity for isopropanol conversion was found to depend on the number of surface acid sites present.
X-Ray Emission Studies of Sub-Picosecond Laser Produced Plasmas (1988)

We report on both experimental and theoretical studies of high-temperature, high-density plasmas produced when a solid is illuminated with intense, 160 femtosecond laser pulses. Time resolved measurements have determined the x-ray pulsewidth from the plasma to be 2 ± 2 psec in all spectral regions up to a kilovolt. Spectroscopic observations demonstrate that the x-ray emission comes from regions of density higher than those found in a conventional laser produced plasma. Finally reflectivity measurements indicate that the laser is absorbed at the surface of the solid before it expands.
Posttraumatiskt Stress Syndrom : kan drabba alla i samhället (2003)

PTSD kan drabba alla i samhallet och darfor bor alla som arbetar inom varden ha en medvetenhet om forekomsten av PTSD och de symtom som personerna kan uppvisa. Syftet med litteraturstudien var att ...
Characterization of human CD7 transgenic mice (1995)

CD7 is a 40-kDa transmembrane glycoprotein member of the lg gene superfamily expressed on most peripheral blood T lymphocytes and NK cells. CD7 is also expressed on myeloid, NK, B, and T cell precursors during adult hematopoiesis. Because Thy-1 is absent in human thymocytes and peripheral blood T cells and shows structural similarities to the human CD7 gene, we have suggested that human CD7 may be a functional homologue in humans of mouse Thy-1. To study the tissue-specific expression of the CD7 gene utilizing its own promoter, we constructed transgenic mice that contained both the coding and flanking regions of the human CD7 gene. We found that human CD7 was expressed in transgenic mice in T, B, NK, and myeloid lineages and was induced with T cell activation. Unlike the expression of CD7 in humans, the CD7 transgene was present on mature B lymphocytes and macrophages. Like mouse Thy-1, transgenic human CD7 was expressed in immature and mature T cells and in Sca-1+ bone marrow mononuclear cells. Unlike mouse Thy-1, the human CD7 transgene was not expressed in mouse brain or fibroblasts. The human CD7 transgene was expressed during fetal development before mouse Thy-1 in fetal liver mononuclear cells. Expression of the human CD7 transgene did not alter mouse thymopiesis or Thy-1 expression. Taken together, these data demonstrated that the CD7 transgene contained sufficient regulatory regions to direct hematopoietic expression and mitogenic induction. The pattern of CD7 transgene expression more closely resembled that of CD7 in humans than that of mouse Thy-1.