gebhart/patrepeval huggingface dataset:
Observation of unphosphorylated STAT3 core protein binding to target dsDNA by PEMSA and X‐ray crystallography (2013)

The STAT3 transcription factor plays a central role in a wide range of cancer types where it is over-expressed. Previously, phosphorylation of this protein was thought to be a prerequisite for direct binding to DNA. However, we have now shown complete binding of a purified unphosphorylated STAT3 (uSTAT3) core directly to M67 DNA, the high affinity STAT3 target DNA sequence, by a protein electrophoretic mobility shift assay (PEMSA). Binding to M67 DNA was inhibited by addition of increasing concentrations of a phosphotyrosyl peptide. X-ray crystallography demonstrates one mode of binding that is similar to that known for the STAT3 core phosphorylated at Y705.
How do lncRNAs regulate transcription? (2017)

It has recently become apparent that RNA, itself the product of transcription, is a major regulator of the transcriptional process. In particular, long noncoding RNAs (lncRNAs), which are so numerous in eukaryotes, function in many cases as transcriptional regulators. These RNAs function through binding to histone-modifying complexes, to DNA binding proteins (including transcription factors), and even to RNA polymerase II. In other cases, it is the act of lncRNA transcription rather than the lncRNA product that appears to be regulatory. We review recent progress in elucidating the molecular mechanisms by which lncRNAs modulate gene expression and future opportunities in this research field.
Sulfhydryl modification and activation of phenylalanine hydroxylase by dinitrophenyl alkyl disulfide (1987)

A new family of asymmetric thiol-disulfide exchange reagents, the dinitrophenyl alkyl disulfides (DNPSSR), was used to modify rat liver phenylalanine hydroxylase. The results indicate that the enzyme has two different types of reactive sulfhydryl (SH) residues per subunit. One SH residue was modified selectively by a DNPSSR having a neutral and hydrophilic alkyl group, and this modification was accompanied by appreciable activation of enzyme; the other SH residue was modified only by an anionic DNPSSR, and this modification did not result in activation. The catalytic properties of phenylalanine hydroxylase activated by DNPSSR were similar to those of the N-ethylmaleimide- (NEM-) modified enzyme, but the process of activation by DNPSSR was quite different from modification with NEM. An analysis of the reaction kinetics of the modification and of catalysis by the modified enzyme suggests that DNPSSR modification causes a change in the subunit interaction leading to a loss of the negative cooperativity normally seen with phenylalanine hydroxylase.
Minimizing power consumption and complexity in a programmable transmit filter bank for OFDM (2004)

Filter banks are efficient and essential signal processing blocks for design and implementation of multi-rate multi-band communications and signaling. In this paper we analytically study and derive the optimum choice of design parameters and filter bank structure to minimize power consumption and implementation cost for a programmable multi-rate transmit filter bank for OFDM. The optimization is performed on two fronts. We first perform system-level power and complexity analysis to define the optimum choice of filter parameters. Then through a hardware-level optimization, an efficient filter bank structure is introduced that results in at least a factor of 4 power reduction and also a complexity reduction from 6.95GOPS to 1.73GOPS for the multi-rate filter bank over the baseline design.
Electrical characterization of manganite and titanate heterostructures (2014)

As the current memory technology will reach its physical limit within the next decades, innovative concepts have to be developed to ensure future improvements in size, power consumption and costs in data storage. A promising candidate among "Beyond CMOS" technologies is resistive RAM, which is based on the non-volatile and reversible switching of the resistance of a device with the help of an external stimulus. More specific, fast writing and reading operations in resistively switching transition metal oxides seem to be enabled by redox-based mechanisms, which usually go along with the formation of a conducting filament to bridge the insulating matrix. For a few materials, the switching occurs homogeneously over the entire device area, which offers one more degree of freedom in device design. For this switching type, the interface effects between the involved resistively switching oxide and the active electrode play the key role. The main topic of this work is this redox-based interplay between the oxidizable electrode material and a complex oxide in a homogeneously switching ReRAM device, realized for the quaternary transition metal oxide (Pr,Ca)MnO3 as epitaxial model system. In order to obtain high quality epitaxial thin films by pulsed laser deposition, detailed growth studies were performed. In spite of the rather high lattice mismatch of 􀀀2:39% to the SrTiO3 substrate, defect-poor (Pr,Ca)MnO3 could be successfully grown in various layer stacks. For film thicknesses larger than 40 nm, the elastic strain energy becomes sufficient high for relaxation effects. We identified two concurring processes for relaxation, crack formation and the incorporation of edge dislocations, which result in crucially different structural and electrical properties of the (Pr,Ca)MnO3 thin film. After an initial electroforming step, (Pr,Ca)MnO3 with Ti as active top electrode exhibits stable resistive switching properties, for which we could obtain a resistance ratio of one order of magnitude and retention times of at least 300 days. A redox reaction was found to occur at the interface involving an oxygen transfer from the (Pr,Ca)MnO3 to theTi. A change of the valence state on the Ti-side was identified during the forming and also the switching processes by X-ray spectroscopy. The current transport in the devices could be described by a polaron hopping contribution and a serial ohmic resistor for the symmetric low resistive states, and with the presence of a trapezoidal tunnel barrier for the high resistive states. Combining both results, a coherent model was derived to explain the switching effect in (Pr,Ca)MnO3/Ti devices by the formation and bridging of a TiO2 tunnel barrier, naturally formed at the (Pr,Ca)MnO3/Ti interface.
Small uncooled bolometers with a broad spectral response (2018)

This paper reports the infrared spectral responses of 17 and 35 &mu;m uncooled bolometers fabricated at INO. They are measured by making use of an external readout circuit along with a monochromator. As expected, the spectral absorption strongly depends on the bolometer stack as well as the pixel layout. By proper selection of design parameters, the spectral response can be made flat from 3 to 14 &mu;m without significant deterioration of the detector figure of merit.
Entropic death of nonpatterned and nanopatterned polyelectrolyte brushes (2019)

ABSTRACT The stability of nonpatterned and nanopatterned strong polyelectrolyte brushes (PEBs) is studied as a function of both brush character and the properties of a contacting liquid. High‐molecular‐weight PEBs of poly(4‐methyl vinylpyridinium iodide) (PMeVP) are synthesized using surface‐initiated radical‐chain polymerization. Nanopatterned brushes (NPBs) line with pattern sizes ranging from 50 to 200 nm are generated by patterning the initiator layer using deep‐ultraviolet photolithography followed by brush growth initiated from the patterned layer. Homogeneous PEBs with different degrees of charging and grafting densities are exposed to water and salt solutions with different temperatures for different periods. The degradation is monitored through dry‐state ellipsometry and atomic force microscopy measurements. Enhanced degrafting for more strongly swollen polymer brushes can be observed in agreement with an “entropic spring” model. Based on the results of the nonpatterned brushes, the NPBs are exposed to water at different temperatures and external salt content for varying periods of time. Counterintuitively, the NPBs show increased degrafting for smaller patterns, which is attributed to different polymer chain dynamics for nanobrushes and microbrushes. We investigate the influence of thermodynamic and kinetic parameters on the stability of (nanopatterned) PEBs and discuss the role of entanglements and formation of complexes in such films. © 2019 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2019, 57, 1283–1295
First-Principles Hydrothermal Synthesis Design to Optimize Conditions and Increase the Yield of Quaternary Heteroanionic Oxychalcogenides (2021)

Hydrothermal synthesis exploits solvent properties, temperature, and pressure to control reaction equilibrium among heterogeneous phases to promote the formation of targeted products, often single-anion-based ceramics (homoanionic materials). Heteroanionic materials with more than one anion present greater phase competition in the hydrothermal medium, making it more challenging to ascertain optimal processing variables. Here, we present a series of hydrothermal syntheses informed by first-principles thermodynamic models, which account for synthesis conditions (reagent concentration, pH, and temperature), and achieve four complex bismuth oxychalcogenides, BiMOQ (M = Cu, Ag; Q = S, Se) in high yield. Our computation-prior-to-experimentation procedure utilizes single-element and multi-element electrochemical (pH-potential) diagrams computed using density functional theory at (non)standard states. We construct stability diagrams to identify the optimal synthesis conditions for the formation of thermodynamically stable phases by requiring product yields of >90% on the stability diagrams. Furthermore, we calculate the most likely byproducts to occur in each system by analyzing reaction driving forces. Last, we synthesize the oxychalcogenides guided by these hydrothermal processing conditions and explain their yield successes based on materials-chemistry differences. Our work provides a strategy to understand, accelerate, and devise de novo hydrothermal syntheses of heteroanionic and chemically complex materials by design prior to experimentation.
Cascading One-pot Synthesis of Biodegradable Uronic Acid-based Surfactants from Oligoalginates, Semi-Refined Alginates and Crude Brown Seaweeds (2023)

The present article describes a one-pot and cascade mode process using biocompatible/biodegradable reagents, for directly and simply obtaining surfactant compositions of mixtures of D-mannuronic acid and L-guluronic acid directly from oligoalginates or semi-refined alginates (mixtures of alginate, cellulose, hemicellulose, laminaran and fucan). Simple treatments of partial purification of the reaction crudes (elimination of the salts and/or the residual fatty alcohols) or isolation of the surfactant compositions result in derived compounds and in compositions having performance levels appropriate to applications in detergency. In addition, the challenging extension of this cascading one-pot synthesis technology to crude milled brown seaweeds was successfully carried out to provide promising surface-active compositions made up of alkyl uronate and alkyl glycoside monosaccharides.
Assessment of the Role of the Glutathione and Pentose Phosphate Pathways in the Protection of Primary Cerebrocortical Cultures from Oxidative Stress (1996)

Reactive oxygen species have been implicated in neuronal injury associated with various neuropathological disorders. However, little is known regarding the relationship between antioxidant enzyme capacity and resultant toxicity. The antioxidant pathways of primary cerebrocortical cultures were directly examined using a novel technique that measures pentose phosphate pathway (PPP) activity, which is enzymatically coupled to glutathione peroxidase (GPx) detoxification of hydrogen peroxide (H2O2). PPP activity was quantified from data obtained by gas chromatography/mass spectrometry analysis of released labeled lactate following metabolic degradation of [1,6-(13)C2, 6,6-(2)H2] glucose by cerebrocortical cultures. The antioxidant capacity of these cultures was systematically evaluated using H2O2, and the resultant toxicity was quantified by lactate dehydrogenase release. Exposure of primary mixed and purified astrocytic cultures to H2O2 caused stimulation of PPP activity in a concentration-dependent fashion from 0.25 to 22.2% and from 6.9 to 66.7% of glucose metabolized to lactate through the PPP, respectively. In the mixed cultures, chelation of iron before H2O2 exposure was protective and resulted in a correlation between PPP saturation and toxicity. Conversely, addition of iron, inhibition of GPx, or depletion of glutathione decreased H2O2-induced PPP stimulation and increased toxicity. These results implicate the Fenton reaction, reflect the pivotal role of GPx in H2O2 detoxification, and contribute to our understanding of the etiological role of free radicals in neuropathological conditions.
A generalized method for evaluating the metallization thickness effects on microstrip structures (2002)

This paper presents a generalized method for analyzing microstrip structures with finite metallization thickness. A spectral-domain approach that adopts proper current expansion functions is employed to find the three-dimensional current distribution in a finitely thick microstrip. Comparison of computed dispersion curves with available published data for microstrip lines of finite thickness illustrates the accuracy of this method. The influence of metallization thickness on discontinuities like patches and open-ends is also demonstrated.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
ChemInform Abstract: PREPARATION, PROPERTIES, AND CRYSTAL AND MOLECULAR STRUCTURES OF BIS(DIALKYLAMINE) COMPLEXES OF RHENIUM(I) (1982)

Abstract Durch Reaktion von Re 2 Br 2 (CO) 6 (THF) 2 in einer Toluol‐ Suspension mit NHEt 2 wurde der Komplex (Ib) dargestellt, der ebenso wie die bereits bekannte Verbindung?a) mit Z=4 in der Raumgruppe P2′, /n kristallisiert.
Automated definition of mid-sagittal planes for MRI brain scans (2007)

In most magnetic resonance imaging (MRI) clinical examinations, the orientation and position of diagnostic scans are manually defined by MRI operators. To accelerate the workflow, algorithms have been proposed to automate the definition of the MRI scanning planes. A mid-sagittal plane (MSP), which separates the two cerebral hemispheres, is commonly used to align MRI neurological scans, since it standardizes the visualization of important anatomy. We propose an algorithm to define the MSP automatically based on lines separating the cerebral hemispheres in 2D coronal and transverse images. Challenges to the automatic definition of separation lines are disturbances from the inclusion of the shoulder, and the asymmetry of the brain. The proposed algorithm first detects the position of the head by fitting an ellipse that maximizes the image gradient magnitude in the boundary region of the ellipse. A symmetrical axis is then established which minimizes the difference between the image on either side of the axis. The pixels at the space between the hemispheres are located in the adjacent area of the symmetrical axis, and a linear regression with robust weights defines a line that best separates the two hemispheres. The geometry of MSP is calculated based on the separation lines in the coronal and transverse views. Experiments on 100 images indicate that the result of the proposed algorithm is consistent with the results obtained by domain experts and is significantly faster.
Generation of correlated photons in nanoscale silicon waveguides (2006)

.We experimentally study the generation of correlated pairs of photons through four-wave mixing (FWM) in embedded silicon waveguides. The waveguides, which are designed to exhibit anomalous group-velocity dispersion at wavelengths near 1555 nm, allow phase matched FWM and thus efficient pair-wise generation of non-degenerate signal and idler photons. Photon counting measurements yield a coincidence-to-accidental ratio (CAR) of around 25 for a signal (idler) photon production rate of about 0.05 per pulse. We characterize the variation in CAR as a function of pump power and pump-to-sideband wavelength detuning. These measurements represent a first step towards the development of tools for quantum information processing which are based on CMOS-compatible, silicon-on-insulator technology.
Gaussian curvature and stigmatic imaging relations for the design of an unobscured reflective relay (2018)

We derive the relationship between Coddington's equations and the Gaussian curvature for a stigmatic reflective imaging system. This relationship allows parameterizing off-axis conic optical systems using traditional first-order optics by considering the effective curvature at the center of the off-axis sections. Specifically, we demonstrate parameterizing the system requirements of a 2× achromatic image relay for a terawatt laser system. This system required both collimation (far-field) and pupil imaging (near-field) simultaneously. Long working distances and specific spatial constraints limited the available layout options for the imaging components. By parameterizing these system requirements and packaging constraints, the final specifications could be quickly iterated, while allowing for flexibility in the layout of the system during a multi-year conceptual period.
Benzylidene hydrazides as potential anticancer agents (1970)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBenzylidene hydrazides as potential anticancer agentsDavid W. Boykin Jr. and Rajendra S. VarmaCite this: J. Med. Chem. 1970, 13, 3, 583–584Publication Date (Print):May 1, 1970Publication History Published online1 May 2002Published inissue 1 May 1970https://doi.org/10.1021/jm00297a072RIGHTS & PERMISSIONSArticle Views61Altmetric-Citations4LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (214 KB) Get e-Alerts Get e-Alerts
Phase I trial of H65-RTA immunoconjugate in patients with cutaneous T- cell lymphoma (1991)

H65-RTA is an immunoconjugate that consists of the A chain of ricin (RTA), a ribosomal-inhibiting protein, coupled to a murine monoclonal antibody (H65) directed against the pan-T-cell antigen CD5. The CD5 antigen is heterogeneously expressed on cutaneous T-cell lymphoma tumor cells, but is not expressed on normal cells except lymphocytes. A phase I trial was therefore conducted in which 14 patients with cutaneous T- cell lymphoma progressive on other therapies were treated with up to three cycles of H65-RTA. The maximal tolerated dose (MTD) of H65-RTA was 0.33 mg/kg/d administered intravenously for 10 days as defined by dyspnea at rest at higher doses. Other reversible side effects included myalgia, mild hypoalbuminemia with weight gain, pedal edema, fatigue, fevers, and chills. Six patients received more than one cycle of H65- RTA without increased side effects compared with the first cycle. Pharmacokinetic analysis showed that peak serum drug levels were dose- dependent, and ranged from 1.13 to 5.56 micrograms/mL, with a terminal half-life ranging from 1.0 to 2.9 hours. The development of antibodies against the immunoconjugate was associated with a lower peak drug level, but not with enhanced side effects. Partial responses lasting from 3 to 8 months were documented in four patients. Three of the responding patients received more than one cycle of H65-RTA in the presence of anti-immunoconjugate antibodies. The results from this phase I trial suggest that H65-RTA is an active drug in the treatment of cutaneous T-cell lymphoma. The immunoconjugate may be safely administered repeatedly, even in the presence of anti-immunoconjugate antibodies, with responses noted. Additional studies at the MTD are needed to define the response rate in this disease.
Innentitelbild: Fluorescent Nanowires Self‐Assembled through Host–Guest Interactions in Modified Calcein (Angew. Chem. 32/2011) (2011)

Stark fluoreszierende Nanodrähte selbstorganisieren in wässriger Lösung aufgrund von Wirt-Gast-Wechselwirkungen zwischen Cyclodextrin- und Adamantan-modifiziertem Calcein. In ihrer Zuschrift auf S. 7545 ff. zeigen H. Ritter und Mitarbeiter mit Transmissions- und Rasterelektronenmikroskopie, dass die Kombination dieser modifizierten fluoreszierenden Farbstoffe mikrometerlange Drähte liefert, die Anwendungen in der Mikrobiologie finden können.
Differential Display Identification of plunc, a Novel Gene Expressed in Embryonic Palate, Nasal Epithelium, and Adult Lung (1999)

We have identified a novel gene transcript of approximately 1.1 kilobases in length that is expressed in the presumptive nasal epithelium of the mouse embryo. In situ hybridization analysis shows discrete regions of expression associated with the palate, nasal septum, and nasal conchae. This transcript is also expressed strongly in the trachea and bronchi of the adult lung. Screening of a mouse heart cDNA library yielded several overlapping clones to give a continuous sequence of 1113 bases, containing an open reading frame of 278 codons comprising the complete mRNA. No significant homologies with known genes were observed at the nucleotide level; limited amino acid homology with two salivary gland-specific proteins was noted. A search for functionally significant protein motifs revealed consensus sequences for N-glycosylation, protein kinase C and casein kinase phosphorylation, and a leucine zipper. Additionally, we observed a unique amino acid sequence pattern, consisting of the residues Gly-(Leu/Pro/Gln)-(Pro/Leu)-Leu-Pro-Leu, repeated four times near the amino-terminal portion of the protein with two amino acid residues separating the repeats. Based on these observations, we propose that we have identified a new gene, which we call plunc (for palate, lung, and nasal epithelium clone; GenBankTM accession number U69172).
Rheology of long‐chain randomly branched polybutadiene (1996)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation Leo J. Kasehagen, Christopher W. Macosko, Dave Trowbridge, Fred Magnus; Rheology of long‐chain randomly branched polybutadiene. J. Rheol. 1 July 1996; 40 (4): 689–709. https://doi.org/10.1122/1.550732 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentThe Society of RheologyJournal of Rheology Search Advanced Search |Citation Search
Efficacy and Safety of Dapagliflozin According to Frailty in Patients With Heart Failure: A Prespecified Analysis of the DELIVER Trial (2022)

gov; Unique identifier: NCT03619213.