gebhart/patrepeval huggingface dataset:
Persistent Infection of Human Pancreatic Islets by Coxsackievirus B Is Associated with Alpha Interferon Synthesis in β Cells (2000)

The interactions of coxsackievirus B3 (CVB3), CVB4E2 (diabetogenic), and CVB4JBV (nondiabetogenic) strains with human pancreatic islets from eight adult brain-dead donors were investigated. Persistent replication of viruses in human islets was proved by detection of viral RNA by in situ hybridization, VP1 capsid protein by immunofluorescence (IF) staining, negative-strand viral RNA by reverse transcription-PCR in extracted RNA from islets, and release of infectious particles up to 30 days after infection without obvious cytolysis. By double IF staining, glucagon-containing alpha cells and insulin-containing beta cells were shown to be susceptible to CVB. The persistence of CVB3 and CVB4 in islet cells was associated with the chronic synthesis of alpha interferon (IFN-alpha), as evidenced by the detection of IFN-alpha mRNA and immunoreactive IFN-alpha with antiviral activity. By double IF staining, IFN-alpha was detected in insulin-producing beta cells only. Experiments with neutralizing anti-coxsackievirus and adenovirus receptor (CAR) antibodies provided evidence that CAR was expressed by alpha and beta cells and that it played a role in the infection of these cells with CVB and the consecutive IFN-alpha expression in beta cells. The viral replication and the expression of IFN-alpha in islets were not restricted to the CVB4E2 diabetogenic strain and did not depend on the genetic background of the host. The neutralization of endogenous IFN-alpha significantly enhanced the CVB replication in islet cells and resulted in rapid destruction of islets. Thus, human beta cells can harbor a persistent CVB infection, and CVB-induced IFN-alpha plays a role in the initiation and/or maintenance of chronic CVB infection in human islets.
Investigation of Pressing and Sintering Processes of CP-Ti Powder Made by Armstrong Process (2010)

This work used in-situ and ex-situ techniques to investigate the pressing and sintering processes of commercially pure (CP) Ti powder made by the Armstrong process. The objective is to simulate the actual manufacturing process of near net shape Ti components. Ti powders were uniaxially pre-pressed at designated pressures up to 100 ksi to form disk samples with different theoretical densities. Compression tests were performed in an SEM at different temperatures to obtain the mechanical properties and deformation behavior of these samples. Ex-situ technique was used to track the powder deformation process of disk samples from low pressure to high pressure. In-situ sintering was also performed in an SEM to record the morphology change of the porosities on the sample surface during the sintering process. The results will provide valuable information for optimizing the manufacturing process of high-density near net shape Ti components.
Does per‐act HIV‐1 transmission risk through anal sex vary by gender? An updated systematic review and meta‐analysis (2018)

Quantifying HIV-1 transmission risk per-act of anal intercourse (AI) is important for HIV-1 prevention. We updated previous reviews by searching Medline and Embase to 02/2018. We derived pooled estimates of receptive AI (URAI) and insertive AI (UIAI) risk unprotected by condoms using random-effects models. Subgroup analyses were conducted by gender, study design, and whether antiretroviral treatment (ART) had been introduced by the time of the study. Two new relevant studies were identified, one of which met inclusion criteria, adding three new cohorts and increasing number of individuals/partnerships included from 1869 to 14 277. Four studies, all from high-income countries, were included. Pooled HIV-1 risk was higher for URAI (1.25%, 95% CI 0.55%-2.23%, N = 5, I<sup>2</sup> = 87%) than UIAI (0.17%, 95 % CI 0.09%-0.26%, N = 3, I<sup>2</sup> = 0%). The sole heterosexual URAI estimate (3.38%, 95% CI 1.85%-4.91%), from a study of 72 women published in a peer-reviewed journal, was significantly higher than the men-who-have-sex-with-men (MSM) pooled estimate (0.75%, 95% CI 0.56%-0.98%, N = 4, P < 0.0001) and higher than the only other heterosexual estimate identified (0.4%, 95% CI 0.08%-2.0%, based on 59 women, excluded for being a pre-2013 abstract). Pooled per-act URAI risk varied by study design (retrospective-partner studies: 2.56%, 95% CI 1.20%-4.42%, N = 2 (one MSM, one heterosexual); prospective studies: 0.71%, 95% CI 0.51%-0.93%, N = 3 MSM, P < 0.0001). URAI risk was lower for studies conducted in the ART era (0.75%, 95% CI 0.52%-1.03%) than pre-ART (1.67%, 95% CI 0.44%-3.67%) but not significantly so (P = 0.537). Prevention messages must emphasize that HIV-1 infectiousness through AI remains high, even in the ART era. Further studies, particularly among heterosexual populations and in resource-limited settings, are required to elucidate whether AI risk differs by gender, region and following population-level ART scale-up.
Performance of Micro-Modem PSK signaling under variable conditions during the 2008 RACE and SPACE experiments (2009)

During the ONR-funded acoustic communications experiments conducted in Rhode Island and Massachusetts in 2008 more than ten investigators participated with new signals and receivers with the intent of establishing performance for next-generation acoustic communications systems. In addition to testing these new approaches, the signals developed and used for the WHOI Micro-Modem over the past ten years were also transmitted and collected. These signals serve as a benchmark for the current generation of multi-channel algorithms that use a decision-feedback equalizer with least-mean squares adaptation which can run in real-time on a low-power DSP. Performance was tested using a QPSK signal with 5 kHz bandwidth and error-correction coding that results in approximately 5000 bps burst throughput in the actual modem.
Unilateral Cryptorchidism in Mice Mutant forPtgds (2012)

The pathophysiology of cryptorchidism, abnormal testicular descent, remains poorly understood. In this study, we show that both heterozygous and homozygous mice deficient for lipocalin-type prostaglandin D(2) (PGD(2) ) synthase (Ptgds) presented unilateral cryptorchidism affecting the second phase of testicular descent in 16% and 24% of cases, respectively. The adult cryptorchid testes show an increase in spermatogonia apoptosis along with a global decrease in the tubule size parameters, whereas the gubernaculum of newborn mutants present some histological abnormalities. Disruption of the inguinoscrotal phase did not present impairment of the androgen pathway but rather a decrease in Rxfp2 mRNA expression in the gubernaculum. These observations led us to investigate the role of the PGD(2) signaling pathway in human testicular migration through PTGDS sequencing of DNA from 29 children with cryptorchidism. However, none of the investigated cases presented mutations in the PTGDS gene. Nevertheless, our results identify the PTGDS enzyme as a novel component in the cryptorchidism puzzle.
Heat Transfer Measurements and Predictions for a Modern, High-Pressure, Transonic Turbine, Including Endwalls (2009)

This paper presents both measurements and predictions of the hot-gas-side heat transfer to a modern, 112 stage high-pressure, transonic turbine. Comparisons of the predicted and measured heat transfer are presented for each airfoil at three locations, as well as on the various endwalls and rotor tip. The measurements were performed using the Ohio State University Gas Turbine Laboratory Test Facility (TTF). The research program utilized an uncooled turbine stage at a range of operating conditions representative of the engine: in terms of corrected speed, flow function, stage pressure ratio, and gas-to-metal temperature ratio. All three airfoils were heavily instrumented for both pressure and heat transfer measurements at multiple locations. A 3D, compressible, Reynolds-averaged Navier–Stokes computational fluid dynamics (CFD) solver with k-ω turbulence modeling was used for the CFD predictions. The entire 112 stage turbine was solved using a single computation, at two different Reynolds numbers. The CFD solutions were steady, with tangentially mass-averaged inlet/exit boundary condition profiles exchanged between adjacent airfoil-rows. Overall, the CFD heat transfer predictions compared very favorably with both the global operation of the turbine and with the local measurements of heat transfer. A discussion of the features of the turbine heat transfer distributions, and their association with the corresponding flow-physics, has been included.
Age, Obesity, Medical Comorbidities and Surgical Technique are Predictive of Symptomatic Anastomotic Strictures After Contemporary Radical Prostatectomy (2011)

Patient factors as well as technical factors influence the development of symptomatic anastomotic strictures following contemporary radical prostatectomy. The impact of these factors is influenced by the individual surgeon and the approach used.
CFD Study of Effects of Module Geometry on Forced Convection in a Channel with Non‐Conducting Fins and Flow Pulsation (2010)

Abstract CFD simulations were carried out to investigate the effects of the module geometry on forced convection in a rectangular channel containing series of regularly spaced non‐conducting baffles with flow oscillation. The simulations were performed at constant wall temperature. Steady‐flow Reynolds numbers Re in the range of 200 and 600 were studied. The results of the CFD simulations show that, for the effect fin spacing to be significant on heat transfer enhancement in finned system with oscillating flow, the oscillating flow velocity must be higher than the mean flow velocity. Superposition of oscillation yields increasing heat transfer performance with increasing fin height. Fin geometry with pyramidal shape yields highest performance in terms of the heat transfer effectiveness.
A genetic-algorithm approach to simulating human immunodeficiency virus evolution reveals the strong impact of multiply infected cells and recombination (2005)

It has been previously shown that the majority of human immunodeficiency virus type 1 (HIV-1)-infected splenocytes can harbour multiple, divergent proviruses with a copy number ranging from one to eight. This implies that, besides point mutations, recombination should be considered as an important mechanism in the evolution of HIV within an infected host. To explore in detail the possible contributions of multi-infection and recombination to HIV evolution, the effects of major microscopic parameters of HIV replication (i.e. the point-mutation rate, the crossover number, the recombination rate and the provirus copy number) on macroscopic characteristics (such as the Hamming distance and the abundance of n-point mutants) have been simulated in silico. Simulations predict that multiple provirus copies per infected cell and recombination act in synergy to speed up the development of sequence diversity. Point mutations can be fixed for some time without fitness selection. The time needed for the selection of multiple mutations with increased fitness is highly variable, supporting the view that stochastic processes may contribute substantially to the kinetics of HIV variation in vivo.
Development of Elemental Technologies for Advanced Humid Air Turbine System (2006)

The Advanced Humid Air Turbine (AHAT) system improves the thermal efficiency of gas turbine power generation by using a humidifier, a Water Atomization Cooling (WAC) system, and a heat recovery system, thus eliminating the need for an extremely high firing temperature and pressure ratio. The following elemental technologies have been developed to realize the AHAT system: (1) a broad working range and high-efficiency compressor that utilizes the WAC system to reduce compression work, (2) turbine blade cooling techniques that can withstand high heat flux due to high-humidity working gas, and (3) a combustor that achieves both low NOx emissions and a stable flame condition with high-humidity air. A gas turbine equipped with a two-stage radial compressor (with a pressure ratio of 8), two-stage axial turbine, and a reverse-flow type of single-can combustor has been developed based on the elemental technologies described above. A pilot plant that consists of a gas turbine generator, recuperator, humidification tower, water recovery system, WAC system, economizer, and other components is planned to be constructed, with testing slated to begin in October 2006 to validate the performance and reliability of the AHAT system. The expected performance is as follows: thermal efficiency of 43% (LHV), output of 3.6 MW, and NOx emissions of less than 10 ppm at 15% O2. This paper introduces the elemental technologies and the pilot plant to be built for the AHAT system.
Oligonucleotide Bioconjugation with Bifunctional Palladium Reagents (2021)

Organometallic reagents enable practical strategies for bioconjugation. Innovations in the design of water-soluble ligands and the enhancement of reaction rates have allowed for chemoselective cross-coupling reactions of peptides and proteins to be carried out in water. There are currently no organometallic-based methods for oligonucleotide bioconjugation to other biomolecules. Here we report bifunctional palladium(II)-oxidative addition complexes (OACs) as reagents for high-yielding oligonucleotide bioconjugation reactions. These bifunctional OACs react chemoselectively with amine-modified oligonucleotides to generate the first isolable, bench stable oligonucleotide-palladium(II) OACs. These complexes undergo site-selective C-S arylation with a broad range of native thiol-containing biomolecules at low micromolar concentrations in under one hour. This approach provided oligonucleotide-peptide, oligonucleotide-protein, oligonucleotide-small molecule, and oligonucleotide-oligonucleotide conjugates in >80 % yield and afforded conjugation of multiple copies of oligonucleotides onto a monoclonal antibody.
Aminoacyl−Anthraquinone Conjugates as Telomerase Inhibitors: Synthesis, Biophysical and Biological Evaluation (2008)

The telomerase-telomere complex is a prospective anticancer target. To inhibit enzyme activity by induction of G-quadruplex in human telomeres, we have synthesized a small library of 2,6- and 2,7-amino-acyl/ peptidyl anthraquinones with diverse connecting linkers, charge, lipophilicity and bulk. The test compounds modulated G-quadruplex stability to different extents and showed clear preference for quadruplex over duplex DNA. Telomerase inhibition correlated with G-quadruplex stabilization. A SAR analysis showed that type of linkage between the linker and the anthraquinone, together with the position of the side chains and the nature of the amino acid components play a major role both in stabilizing G-quadruplex and producing telomerase inhibition. Short-term cytotoxic activity was poor. However, after prolonged exposure to effective G-quadruplex binders, cells became senescent. These results are of help in the rational design of more efficient G-quadruplex stabilizers, possibly endowed with cancer cell-selective antiproliferative effects.
Color Enrichment Solids of Spectrally Pure Colloidal Quantum Wells for Wide Color Span in Displays (2022)

Abstract Colloidal quantum wells (CQWs) are excellent candidates for lighting and display applications owing to their narrow emission linewidths (&lt;30 nm). However, realizing their efficient and stable light‐emitting solids remains a challenge. To address this problem, stable, efficient solids of CQWs incorporated into crystal matrices are shown. Green‐emitting CdSe/CdS core/crown and red‐emitting CdSe/CdS core/shell CQWs wrapped into these crystal solids are employed as proof‐of‐concept demonstrations of light‐emitting diode (LED) integration targeting a wide color span in display backlighting. The quantum yield of the green‐ and red‐emitting CQW‐containing solids of sucrose reach ≈20% and ≈55%, respectively, while emission linewidths and peak wavelengths remain almost unaltered. Furthermore, sucrose matrix preserves ≈70% and ≈45% of the initial emission intensity of the green‐ and red‐emitting CQWs after &gt;60 h, respectively, which is ≈4× and ≈2× better than the drop‐casted CQW films and reference (KCl) host. Color‐converting LEDs of these green‐ and red‐emitting CQWs in sucrose possess luminous efficiencies 122 and 189 lm W −1 elect , respectively. With the liquid crystal display filters, this becomes 39 and 86 lm W −1 elect , respectively, providing with a color gamut 25% broader than the National Television Standards Committee standard. These results prove that CQW solids enable efficient and stable color converters for display and lighting applications.
<title>Particle filter-based track before detect algorithms</title> (2003)

In this paper we will give a general system setup, that allows the formulation of a wide range of Track Before Detect (TBD) problems. A general basic particle filter algorithm for this system is also provided. TBD is a technique, where tracks are produced directly on the basis of raw (radar) measurements, e.g. power or IQ data, without intermediate processing and decision making. The advantage over classical tracking is that the full information is integrated over time, this leads to a better detection and tracking performance, especially for weak targets. In this paper we look at the filtering and the detection aspect of TBD. We will formulate a detection result, that allows the user to implement any optimal detector in terms of the weights of a running particle filter. We will give a theoretical as well as a numerical (experimental) justification for this. Furthermore, we show that the TBD setup, that is chosen in this paper, allows a straightforward extension to the multi-target case. This easy extension is also due to the fact that the implementation of the solution is by means of a particle filter.
ChemInform Abstract: ERGOLINE SYNTHONS. 2. SYNTHESIS OF 1,5‐DIHYDROBENZ(CD)INDOL‐4(3H)‐ONES AND 1,3,4,5‐TETRAHYDROBENZ(CD)INDOL‐4‐AMINES (1985)

Abstract Der Methoxy‐indolcarbonsäureester (I) wird zu (‐II)‐(IV) je nach Bedingungen bromiert.
Benzo[f]quinazoline Inhibitors of Thymidylate Synthase: Methyleneamino-Linked Aroylglutamate Derivatives (1994)

Syntheses of several new inhibitors of thymidylate synthase (TS) structurally related to folic acid are described in which the pterin portion of the folate molecule is replaced by a benzo[f]quinazoline moiety, but which retain the natural methyleneamino link to the benzoylglutamate side chain. The effect on enzyme activity and cytotoxicity of various changes in the structure of the (p-aminobenzoyl)glutamate side chain are reported. Replacement of the benzamide portion of the (p-aminobenzoyl)glutamate moiety with 2-fluorobenzamido, 2-isoindolinyl, 1,2-benzisothiazol-2-yl, and 2-thenamido moieties varied in effect from a 9-fold diminution of TS activity to a 5-fold enhancement, while cytotoxic potency on SW-480 and MCF-7 tumor lines showed increases ranging from 3.6- to 450-fold. The detrimental effect on enzyme activity and cytotoxicity of alkyl substitution on the PABA nitrogen is confirmed for these compounds, in contrast with several series of previously reported quinazoline antifolates (2). Substitution of a C3-methyl substituent for 3-amino had little effect on TS activity but was beneficial in terms of solubility and cytotoxicity. The excellent combination of TS inhibitory activity, FPGS substrate activity, and affinity for the reduced folate transport system in the most potent of these derivatives, 3e, resulted in IC50 values of 0.2-0.8 nM against these tumor lines.
Thermoplastic lignocellulose materials: A review on recent advancement and utilities (2023)

Lignocellulosic biomass is the most abundantly available resource in nature. However, its potential as a replacement of oil in plastic production has not been fully exploited. To reduce the carbon footprint, the use of lignocellulose biomass to produce bio-based plastics is attracting increasing global interest. The aim of this review article is to systematically summarize the recent advancements of the development of lignocellulose materials that possess thermoplastic properties, meaning they can be processed/shaped by common plastic processing techniques. The approaches used for modification of lignocellulose biomass and the properties of the modified materials, as well as factors affecting the properties of these, are discussed. The regulatory aspects and policy directions of bio-based plastics, including thermoplastic lignocellulose, are also mentioned. Current challenges of producing thermoplastic lignocellulose and the way forward to solve this are also explored.
A Brillouin gain based fast light fiber laser for sensing applications (2012)

We present a novel superluminal fiber laser based super-sensor employing Brillouin gain. The white light cavity condition is attained by introducing an additional ring cavity into the main cavity. By adjusting the parameters of the laser cavity and those of the phase component it is possible to attain sensitivity enhancement of more than two orders of magnitude compared to conventional laser sensors. The tradeoffs between the attainable sensitivity enhancement, the cavity dimensions and the impact of the cavity roundtrip loss are studied in details providing a set of design rules for the optimization of the super-sensor.
Photo-cross-linking and identification of nuclear proteins that bind to DNA containing a site-specific adduct of cis-[Pt(NH₃)(N-6-aminohexyl)-4-benzophenonamide)CI₂] (2008)

Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 2008.
Altered immune responses in mice without initial dermal lymphatics (2009)

Reference EPFL-CONF-159330View record in Web of Science Record created on 2010-11-30, modified on 2017-05-12
A tilting device for three‐dimensional microscopy: Application to in situ imaging of interphase cell nuclei (1992)

The resolution of an optical microscope is considerably less in the direction of the optical axis (z) than in the focal plane (x-y plane). This is true of conventional as well as confocal microscopes. For quantitative microscopy, for instance studies of the three-dimensional (3-D) organization of chromosomes in human interphase cell nuclei, the 3-D image must be reconstructed by a point spread function or an optical transfer function with careful consideration of the properties of the imaging system. To alleviate the reconstruction problem, a tilting device was developed so that several data sets of the same cell nucleus under different views could be registered. The 3-D information was obtained from a series of optical sections with a Zeiss transmission light microscope Axiomat using a stage with a computer-controlled stepping motor for movement in the z-axis. The tilting device on the Axiomat stage could turn a cell nucleus through any desired angle and also provide movement in the x-y direction. The technique was applied to 3-D imaging of human lymphocyte cell nuclei, which were labelled by in situ hybridization with the DNA probe pUC 1.77 (mainly specific for chromosome 1). For each nucleus, 3-D data sets were registered at viewing angles of 0 degrees, 90 degrees and 180 degrees; the volumes and positions of the labelled regions (spots) were calculated. The results also confirm that, in principle, any angle of a 2 pi geometry can be fixed for data acquisition with a high reproducibility. This indicates the feasibility of axiotomographical microscopy of cell nuclei.