gebhart/patrepeval huggingface dataset:
Endothelial caveolar modulation of SK3 channel activity (2013)

Small‐ and intermediate‐conductance Ca2+‐activated K+ channels (SK3/Kcnn3 and IK1/Kcnn4) are expressed in vascular endothelium. Their activities play important roles in regulating vascular tone through their modulation of [Ca2+]i required for the production of endothelium‐derived vasoactive agents. Activation of endothelial IK1 or SK3 channels hyperpolarizes endothelial cell membrane potential, increases Ca2+ influx, and leads to the release of vasoactive factors, thereby impacting blood pressure. To examine the distinct roles of IK1 and SK3 channels, we used electrophysiological recordings to investigate IK1 and SK3 channel trafficking in acutely dissociated endothelial cells from mouse aorta. The results show that SK3 channels undergo Ca2+‐dependent cycling between the plasma membrane and intracellular organelles; disrupting Ca2+‐dependent endothelial caveolae cycling abolishes SK3 channel trafficking. Moreover, transmitter induced changes in SK3 channel activity and surface expression modulate endothelial membrane potential. In contrast, IK1 channels do not undergo rapid trafficking and their activity remains unchanged when either exo‐ or endocytosis is block. Thus, modulation of SK3 surface expression may play an important role in regulating endothelial membrane potential in a Ca2+‐dependent manner.
Supplementary Data from Loss of Function of DOCK4 in Myelodysplastic Syndromes Stem Cells is Restored by Inhibitors of DOCK4 Signaling Networks (2023)

&lt;p&gt;Supplemental figure 4: Twenty four hours post siRNA nucleofection immunoblotting analysis was performed to test knockdown of A) LYN, B) SHP1 and C) SHIP1 in primary HSCs.&lt;/p&gt;
Abstract 513: Emre Regulates the Mitochondrial Calcium Uniporter in vivo (2020)

Mitochondrial uptake of Ca 2+ plays critical roles in cardiac energy production as well as cell death. The mitochondrial calcium uniporter in mice and humans is a multi-protein complex that includes the channel-forming protein MCU and several other subunit proteins, including EMRE. EMRE is a single transmembrane protein that is conserved among metazoan species and is known to be essential for mitochondrial Ca 2+ uptake in cell culture. To investigate EMRE’s role in organismal physiology, we generated a mouse model of global germline EMRE deletion. We show that EMRE is indeed required for mitochondrial calcium uniporter function in isolated mitochondria from multiple tissues. Although the birth rate of Emre -/- mice is lower than expected by Mendelian genetics (~5-10% instead of ~25%), the mice that are born are viable and appear healthy. Oxygen consumption in isolated mitochondria and cells is not significantly affected by loss of EMRE, and similarly the mice do not exhibit overt metabolic impairment, even under strenuous exercise. No significant differences between Emre -/- and wild-type ( WT ) cardiac function at baseline and after isoproterenol stimulation are evident by echocardiography. Moreover, Emre -/ - hearts are not protected from ischemia/reperfusion injury in a Langendorff perfusion model (mean infarct area 61% in Emre -/- hearts; 57% in WT ). Collectively, these data and their similarities to results found via germline Mcu deletion demonstrate that EMRE is indeed essential for mitochondrial Ca 2+ uptake in vivo. Furthermore, we find evidence that EMRE protein expression is elevated in some mouse muscular dystrophy models, suggesting that modulation of EMRE levels may play a role in regulating uniporter activity in conditions of stress or disease. We therefore further explore whether and how EMRE expression changes with isoproterenol-induced cardiac hypertrophy in mice and in samples from human patients with heart failure. Understanding of how uniporter components such as EMRE can regulate MCU in a diseased state can inform better therapeutic strategies aimed at restoring mitochondrial metabolic homeostasis.
Discrete mechanics optimal control (DMOC) and model predictive control (MPC) synthesis for reaction-diffusion process system with moving actuator (2010)

This paper is concerned with the computation of optimal motion control as well as the optimal input injection policy of an actuator arm regulating the temperature in a reaction-diffusion system. The system has two dynamical components consisting of the arm mechanics with inertial, elastic and damping properties, which is driven by bounded mechanical actuation controls and an underlying reaction-diffusion system described by the parabolic PDE. The state of the actuator arm parametrizes the input injection operator of the parabolic PDE systems model and causes coupling between the two dynamical systems generally operating at different time scales. The method proposed in this paper is aimed at solving this coupled problem. The actuator mechanics and its control are achieved in the discrete mechanics and optimal control (DMOC) framework, while the input injection for the reaction diffusion system is calculated by the modal model predictive control (MMPC) algorithm suitable for the dissipative systems. The actuation arm policy and input to the parabolic PDE system include in its realization low-order discrete representation of the parabolic PDE evolution and incorporate optimality with respect to both the state of the PDE and the actuator displacement cost from current to some more optimal control position as well as naturally present input and PDE state constraints. The proposed actuation arm policy and optimal stabilization of the unstable reaction-diffusion system in the presence of constraints in the full state-feedback controller realization have been evaluated through simulations.
Linear Channel Estimation Based on a Low-Bandwidth Observation Channel\n with Unknown Response (2021)

We propose a novel system identification technique, based on a least-mean\nsquare algorithm, allowing for the estimation of a linear channel by using an\nunknown-response measurement channel. The key of the technique is a memoryless\nnonlinear function working as uncoupling block between the estimated and\nobservation channels, conforming a Wiener-Hammerstein scheme. We prove that\nthis estimation, only differing from the actual channel response by a scaling\nfactor and a temporal shift, does not depend on the observation channel\nbandwidth. As a consequence, this technique enables the usage of low-cost\nmeasurement devices as feedback channel. We present numerical examples of the\nmethod, supporting the proposal and displaying excellent results.\n
Additions and Corrections - Nuclear Magnetic Resonance Spectroscopy. The Configurational Stability of Primary Grignard Reagents. Structure and Medium Effects. (1967)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAdditions and Corrections - Nuclear Magnetic Resonance Spectroscopy. The Configurational Stability of Primary Grignard Reagents. Structure and Medium Effects.George M. Whitesides and John D. RobertsCite this: J. Am. Chem. Soc. 1967, 89, 11, 2799Publication Date (Print):May 1, 1967Publication History Published online7 May 2004Published inissue 1 May 1967https://pubs.acs.org/doi/10.1021/ja00987a603https://doi.org/10.1021/ja00987a603research-articleACS PublicationsRequest reuse permissionsArticle Views38Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Novel acylation catalysts in peptide synthesis: derivatives of N-hydroxytriazoles and N-hydroxytetrazoles (1998)

Six new derivatives of 1-hydroxy-1,2,3-triazole (1-hydroxytriazole in the following) and N-hydroxytetrazole have been evaluated in a direct competition assay to investigate their efficiency as catalysts in the formation of peptide bonds. Furthermore, three well known catalysts, 1-hydroxy-7-azabenzotriazole (HOAt), 3,4-dihydro-3-hydroxy-4-oxo-1,2,3-benzotriazine (Dhbt-OH) and 1-hydroxybenzotriazole (HOBt) have been compared. All nine compounds have been used for activation in combination with N,N′-diisopropylcarbodiimide (DIPCDI) in solid-phase synthesis using the fluoren-9-ylmethoxycarbonyl (Fmoc) strategy. The capability of the catalysts to suppress racemization has also been analysed. The results demonstrate that three of the new compounds are competitive with HOAt and HOBt in the suppression of racemization. 5-Chloro-1-hydroxytriazole is found to be a highly efficient acylation catalyst; however, it does not show sufficient suppression of racemization. Its catalytic effect in the synthesis of Aib–Aib-containing peptides is superior to that of HOAt. Also, 2-hydroxytetrazole has a catalytic efficiency superior to that of HOAt and suppressed racemization as efficiently as HOBt. The hydroxytetrazoles are explosive in a hammer test whereas the triazoles are stable compounds.
A 1.2-V, 12-bit, 200Msample/s current-steering D/A converter in 90-nm CMS (2006)

This paper describes a 12-bit, 200-MSample/s current-steering CMOS D/A converter for wireless-communication terminals. To reduce the effects of voltage drops (IR drops) along power supplies, 3-dimensional wiring is applied. The converter was implemented in a 90-nm CMOS technology, and it operates at a low supply voltage of 1.2 V. The modulator achieves the intrinsic accuracy of 12 bits and a spurious-free dynamic range (SFDR) above 55 dB over a 100-MHz bandwidth
Local magnetoresistance in Fe/MgO/Si lateral spin valve at room temperature (2014)

Room temperature local magnetoresistance in two-terminal scheme is reported. By employing 1.6 nm-thick MgO tunnel barrier, spin injection efficiency is increased, resulting in large non-local magnetoresistance. The magnitude of the non-local magnetoresistance is estimated to be 0.0057 Ω at room temperature. As a result, a clear rectangle signal is observed in local magnetoresistance measurement even at room temperature. We also investigate the origin of local magnetoresistance by measuring the spin accumulation voltage of each contact separately.
Controlling the growth of internet routing tables through market mechanisms (2010)

The growth of core Internet routing tables has been such that it is now viewed as an impediment to the continued expansion of the Internet. The main culprit is multi-homing that stems from sites' desire for greater reliability and diversity in connectivity. These locally rational decisions have a global impact on the Internet, and there is currently no mechanism to effectively control them. A number of technical solutions are being pursued, but this paper explores the use of a "market mechanism." It formulates a model that accounts for sites' incentives and the impact their connectivity choices have on the size of routing tables, and introduces a pricing scheme that seeks to better reapportion the resulting costs. The model is solved for two configurations that capture different deployment realizations and stages. They demonstrate the scheme's effectiveness in controlling the growth of Internet routing tables, while improving the welfare of sites and Internet Service Providers.
Noise Reduction of Common-Source Type Active-Clamped DC-DC Converters (2003)

Several topologies of active-clamped DC-DC converters have been proposed before. This paper compares the common-mode noise properties of the conventional type using a pulse transormer for a gate driver and the common-source type proposed recently. Using a noise measuring system composed of LISN (Line Impedance Stabilization Network) and EMSCAN (Electromagnetic Scanning System), it was clarified that the common-source type has the superiority on noise reduction to the conventional one.
Using Generalized Polyspike Train to Predict Drug-Resistant Idiopathic Generalized Epilepsy (2020)

The findings support the hypothesis that GPT is associated with drug-resistant IGE. Prolonged EEG recording is required to identify this feature. Thus, >24-hour EEG recording early in the evaluation of patients with IGE may facilitate prognostication.
Supplementary Table 1 from Autotaxin and LPA<sub>1</sub> and LPA<sub>5</sub> Receptors Exert Disparate Functions in Tumor Cells versus the Host Tissue Microenvironment in Melanoma Invasion and Metastasis (2023)

&lt;p&gt;Supplementary Table 1. Primer sequences used in quantitative Real-Time PCR analysis.&lt;/p&gt;
SmartSiC™ for Manufacturing of SiC Power Devices (2022)

SmartSiC™ is an alternative to SiC bulk substrate, with ideal characteristics to manufacture SiC Power devices, together with an excellent carbon footprint. SmartSiC substrates will soon be manufactured in high volume. In order to be ready for this, key technical development items were needed to deliver the product performance, which are reported here.
Synthesis of substituted pyrroles via zirconocene complexes of imines (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of substituted pyrroles via zirconocene complexes of iminesStephen L. Buchwald, M. Woods Wannamaker, and Brett T. WatsonCite this: J. Am. Chem. Soc. 1989, 111, 2, 776–777Publication Date (Print):January 1, 1989Publication History Published online1 May 2002Published inissue 1 January 1989https://pubs.acs.org/doi/10.1021/ja00184a080https://doi.org/10.1021/ja00184a080research-articleACS PublicationsRequest reuse permissionsArticle Views766Altmetric-Citations64LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
MD1 Expression Regulates Development of Regulatory T Cells (2006)

Intense interest has centered around the role of a subset of regulatory T cells, CD4+CD25+ Treg, in controlling the development of autoimmune disorders, allograft rejection, infection, malignancy, and allergy. We previously reported that MD1, a molecule known to be important in regulation of expression of RP105, also was important in regulating alloimmunity, and that blockade of expression of MD1 diminished graft rejection in vivo. One mechanism by which an MD1-RP105 complex exerts an effect on immune responses is through interference with an LPS-derived signal delivered through the CD14-MD-2-TLR4 complex. We show below that LPS signaling for Treg induction occurs at higher LPS thresholds that for effector T cell responses. In addition, blockade of MD1 functional activity in dendritic cells (using anti-MD1 mAbs, MD1 antisense deoxyoligonucleotides, or responder cells from mice with deletion of the MD1 gene), resulted in elevated Treg induction in response to allogeneic stimulation (in vivo or in vitro) in the presence of LPS. These data offer one mechanistic explanation for the augmented immunosuppression described following anti-MD1 treatment.
An experimental investigation of active noise control in ducts (1984)

This study was directed to gaining an understanding of the physical mechanism of active noise suppression in ducts for both plane waves and higher order modes. Previous work has indicated large suppressions are attainable using active control concepts for plane progressive wave propagation. However, there is little evidence suggesting the mechanism of suppression. Furthermore, it was of interest to determine whether this concept could be applied to sound carried by higher order modes in ducts. This work utilized NASA Langley's Spinning Mode Synthesizer duct facility to provide the number of control points necessary for higher order mode propagation. Pressure measurement probes were installed between the noise source and control point to provide data to define the suppression mechanism. Results indicated that for all modes suppressions of 25 dB or greater were attainable over a broad frequency range. The mechanism of suppression appeared to be a pressure minimum being forced at the control plane which caused a near perfect reflection to occur, This was true for both primary and control sources, as little net acoustic energy was actually developed.
The Engines of Our Ingenuity (2001)

In The Engines of Our Ingenuity, John Lienhard traces the evolution of technology from prehistory to the modern day. Clearly, in such an extended period it is not possible to be comprehensive in a text of some 250 pages, and therefore we are provided with snippets and windows onto certain highlights, deemed to be descriptive of our ingenuity. Professor Lienhard's own background and experience as a mechanical engineer comes to the fore, and thus many of the selected examples tend to demonstrate evolution that has occurred through the mechanical sciences.
Environment copying system based on real-world haptics (2009)

This paper proposes environment copying system, which stores and reproduces haptic information in the real-world. The proposed system consists of environment saving system and environment loading system. The environment saving system stores haptic information of the environment to a environment data memory. On the other hand, the environment loading system reproduces the environmental haptic sensation using the environment data memory, and stimulates the human operator. The environment copying system proposed in this paper realizes storage and reproduction of real world haptic information without environmental mathematical model. By the experiments, the validity of the proposed method is confirmed. It will be useful for industrial applications, medical and welfare human support.
Adaptive MFR parameter control: fixed vs. variable probabilities of detection (2005)

In this paper an efficient adaptive parameter control scheme for Multi Function Radar (MFR) is used. This scheme has been introduced in.<sup>5</sup> The scheme has been designed in such a way that it meets constraints on specific quantities that are relevant for target tracking while minimizing the energy spent. It is shown here, that this optimal scheme leads to a considerable variation of the realized detection probability, even within a single scenario. We also show that constraining or fixing the probability of detection to a certain predefined value leads to a considerable increase in the energy spent on the target. This holds even when one optimizes the fixed probability of detection. The bottom line message is that the detection probability is not a design parameter by itself, but merely the product of an optimal schedule.
Acoustomicrofluidics for micro-object Manipulation (2016)

Interaction of acoustic waves with fluids at a microscale have shown great potential in realizing efficient actuation of liquids for atomization/nebulization, mixing, pumping, gradient generation, etc. It has also enabled researchers working in various fields to dexterously manipulate suspended micro-objects—microparticles, cells, microorganisms—for concentration, separation, sorting, medium exchange, etc. Surface acoustic waves (SAWs)—travelling as well as standing—have been used to demonstrate several of the above mentioned applications with great success. In particular, standing SAWs along with other bulk acoustic waves-based techniques have been proven to efficiently and continuously separate micro-objects inside a microfluidic channel. Our focus has so far been to explore the unexplored use of travelling SAWs to unravel some of the associated advantages. We have used high frequency travelling SAWs—O(100 MHz)—to separate microparticles flowing through a microchannel or suspended within a sessile droplet. Contrary to the formation of pressure nodes in standing SAWs for micro-object manipulation, traveling SAWs generate a strong acoustic streaming flow and impart a direct acoustic radiation force on the suspended particles for efficient manipulation capabilities with all the additional advantages of their non-invasive nature, label-free manipulation, biocompatibility, low costs, and simplicity in operation.
Insulin receptor‐deficient cells as a new tool for dissecting complex interplay in insulin and insulin‐like growth factors (1998)

Cell systems derived from knockout mice for the insulin receptor (IR) or the IGF-1 receptor (IGF-1R) represent unique tools for dissecting complex interplay in the actions of insulin and insulin-like growth factors through their cognate versus non-cognate receptor. In this study, we used a fibroblast cell line derived from IR-deficient mice to investigate metabolic and mitogenic effects of IGF-1 and insulin. IGF-1 was able to stimulate glucose uptake, glucose incorporation into glycogen and thymidine incorporation in such cells. Phosphatidylinositol 3-kinase and mitogen-activated protein kinase, two enzymes of major metabolic-mitogenic signaling pathways, were activated upon stimulating these cells with IGF-1. All these effects were also achieved when IR-deficient cells were stimulated with insulin. Thus, IGF-1R can represent an alternative receptor through which insulin might exert some of its effects.
FcγRIIB regulates autoantibody responses by limiting marginal zone B cell activation (2022)

FcγRIIB is an inhibitory receptor expressed throughout B cell development. Diminished expression or function is associated with lupus in mice and humans, in particular through an effect on autoantibody production and plasma cell (PC) differentiation. Here, we analyzed the effect of B cell-intrinsic FcγRIIB expression on B cell activation and PC differentiation. Loss of FcγRIIB on B cells in Fcgr2b-conditional KO (Fcgr2b-cKO) mice led to a spontaneous increase in autoantibody titers. This increase was most striking for IgG3, suggestive of increased extrafollicular responses. Marginal zone (MZ) B cells had the highest expression of FcγRIIB in both mice and humans. This high expression of FcγRIIB was linked to increased MZ B cell activation, Erk phosphorylation, and calcium flux in the absence of FcγRIIB triggering. We observed a marked increase in IgG3+ PCs and B cells during extrafollicular PC responses in Fcgr2b-cKO mice. The increased IgG3 response following immunization of Fcgr2b-cKO mice was lost in MZ-deficient Notch2 Fcgr2b-double KO mice. Importantly, patients with systemic lupus erythematosus (SLE) had a decrease in FcγRIIB expression that was strongest in MZ B cells. Thus, we present a model in which high FcγRIIB expression in MZ B cells prevented their hyperactivation and ensuing autoimmunity.