gebhart/patrepeval huggingface dataset:
A key management framework for AMI networks in smart grid (2012)

The environments in which current electric grids operate as well as the requirements for the emerging smart grid differ substantially from those of today's Internet and telecommunication networks. For example, typical electric or gas meters in the advanced metering infrastructure (AMI) are low-power, low-capability wireless devices utilizing personal area wireless network technology (e.g., IEEE 802.15.4). These devices are low-cost, typically with 4-12 kbytes of RAM and 64-256 kbytes of flash memory, and are often connected to the backhaul via low-bandwidth links. Comparing this to the high-powered, high-capability devices of the Internet with gigabytes of RAM and storage, the differences are striking. Security solutions designed for capability-rich Internet devices will not be suitable for the capability-poor devices of the smart grid; thus, new requirements are needed that can efficiently operate in resource constrained devices. With this operating environment in mind, we present a smart grid key management framework with application to AMI networks. Specifically, we describe how this key management model can be realized in such a resource-constrained environment using existing standard protocols and provide preliminary performance results.
A Study on the Effect of Porosity and Particles Size Distribution on Li-Ion Battery Performance (2017)

A pseudo two-dimensional model (P2D) is presented that describes the effect of the structural properties of the positive electrode on Li-ion cell performance during discharge. The validation of the mono-modal model was done by using Doyle's experiment and results [C.M. Doyle, University of California, Berkeley (1995)]. A large increase or decrease in the porosity beyond a specific value led to a sharp change in the cell voltage curve and lower cell capacities. The maximum specific energy was obtained in the porosity range of 0.55, while the specific power still had a high value. Furthermore, different particle size distribution models, including mono-modal, bi-modal and 3-particle models, were compared to each other. The mono-modal model was the ideal state with the lowest total polarization. The bi-modal and 3-particle models approached this ideal state when the volume fraction of the smallest particles in their structures increased. This structural arrangement in these models led to more uniform local current density distribution profiles resulting in a greater decrease in cell polarization. Different discharge current densities were applied to different particle size distribution models, and the results showed that the particle size distribution has a greater effect at higher discharge current densities.
LTBP2gene analysis in theGLC3C-linked family and 94CYP1B1-negative cases with primary congenital glaucoma (2012)

LTBP2 mutations were not found in the Turkish GLC3C-linked PCG family or in 94 British CYP1B1-negative PCG cases. Our data suggest that LTBP2 mutations are not a significant cause for isolated trabeculodysgenesis.
The Leader Sequence from the 5′-Terminus to the A-Protein Initiation Codon in MS2-Virus RNA (1971)

RNA fragments of different chain length, each containing the 5'-terminal guanosine tetraphosphate (pppGp) of bacteriophage-MS2 RNA, have been isolated from partial ribonuclease digests of the viral RNA. The longest fragment overlaps with the ribosomalbinding site of the A-protein cistron. The base sequence has been established for the major part. The results directly confirm that the A-protein cistron is closest to the 5'-terminus. Its initiating (AUG) codon starts at position 130, being preceded by an untranslated sequence of 129 nucleotides.
Palladium-Catalyzed α-Arylation of Ketones (1997)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTPalladium-Catalyzed α-Arylation of KetonesMichael Palucki and Stephen L. BuchwaldView Author Information Department of Chemistry Massachusetts Institute of Technology Cambridge, Massachusetts 02139 Cite this: J. Am. Chem. Soc. 1997, 119, 45, 11108–11109Publication Date (Web):November 12, 1997Publication History Received30 July 1997Published online12 November 1997Published inissue 1 November 1997https://pubs.acs.org/doi/10.1021/ja972593shttps://doi.org/10.1021/ja972593srapid-communicationACS PublicationsCopyright © 1997 American Chemical SocietyRequest reuse permissionsArticle Views13548Altmetric-Citations541LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Anions,Arylation,Ketones,Mixtures,Palladium Get e-Alerts
Tables S2-S3 from Genomic Evolutionary Patterns of Leiomyosarcoma and Liposarcoma (2023)

&lt;p&gt;Supplementary Tables 2 and 3&lt;/p&gt;
A coupled « membrane-AOP » process for water treatment: degradation of acetaminophen (2016)

International audience
Investigation of the count rate performance of General Electric Advance positron emission tomograph (1995)

A series of 2D and 3D count rate measurements have been carried out on the Advance PET system. The measurements include: NEMA standard protocols; simultaneous imaging of two small vials containing [0-15] water and (F-18); a cardiac insert placed in a water filled elliptical tank; two different head sized cylindrical tanks; and a Hoffman 3D phantom. The data were processed with and without deadtime and scatter corrections. Plots were made of the total count rate, randoms, trues+scatter, scatter corrected trues, and noise equivalent count rate as a function of total activity in the machine and as the mu Ci/cc in the phantoms. The accuracy of deadtime corrections was also measured. In 2D mode, the NEMA standard protocol resulted in maximum 'trues' count rates of 540,000 cps in high sensitivity mode. In 3D mode the head sized cylindrical phantoms yielded a maximum trues rate of approximately 950,000 cps. Recovery of the water half-life was accurate with up to 50 mCi for the cardiac insert in 2D mode.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Electrical characterization and application of very high speed vertical cavity surface emitting lasers (VCSELs) (2002)

Vertical Cavity Surface Emitting Lasers (VCSELs) offer many benefits over conventional edge-emitting lasers including economical microelectronic batch processing, easy extension to 2-D arrays, and of interest here, very large intrinsic bandwidths due to reduced cavity volume. Results of electrical characterization of a 19 GHz bandwidth 850 nm VCSEL are presented. Small-signal characterization and modeling of the frequency response and device impedance is presented. Large signal performance is studied using two-tone RF and high-speed digital measurements. Appropriate drive conditions for high-speed digital applications are demonstrated.
Selective, naked cluster cryophotochemistry: trisilver, Ag3 (1979)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSelective, naked cluster cryophotochemistry: trisilver, Ag3Geoffrey A. Ozin, Helmut Huber, and Steven A. MitchellCite this: Inorg. Chem. 1979, 18, 10, 2932–2934Publication Date (Print):October 1, 1979Publication History Published online1 May 2002Published inissue 1 October 1979https://doi.org/10.1021/ic50200a065Request reuse permissionsArticle Views67Altmetric-Citations28LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (436 KB) Get e-Alertsclose Get e-Alerts
Constructing Mutants in Serotype 1 <em>Streptococcus pneumoniae</em> strain 519/43 (2020)

Streptococcus pneumoniae serotype 1 remains a huge problem in low-and-middle income countries, particularly in sub-Saharan Africa. Despite its importance, studies in this serotype have been hindered by the lack of genetic tools to modify it. In this study, we describe a method to genetically modify a serotype 1 clinical isolate (strain 519/43). Interestingly, this was achieved by exploiting the Pneumococcus' ability to naturally acquire DNA. However, unlike most pneumococci, the use of linear DNA was not successful; to mutate this important strain, a suicide plasmid had to be used. This methodology has provided the means for a deeper understanding of this elusive serotype, both in terms of its biology and pathogenicity. To validate the method, the major known pneumococcal toxin, pneumolysin, was mutated because it has a well-known and easy to follow phenotype. We showed that the mutant, as expected, lost its ability to lyse red blood cells. By being able to mutate an important gene in the serotype of interest, we were able to observe different phenotypes for loss of function mutants upon intraperitoneal and intranasal infections from the ones observed for other serotypes. In summary, this study proves that strain 519/43 (serotype 1) can be genetically modified.
Short-range correlations and localization-delocalization transition in metal-dielectric films (2003)

Anderson localization in random potential fields is one of most fundamental problems that plays an important role in a number of physical phenomena [1]. It is well known that in 1D and 2D systems with non-correlated, macroscopically homogeneous random potential distributions, all states are exponentially localized. In this paper we show that the introduction of short-range correlations in random metal-dielectric composites results in the presence of delocalized states in the spectrum. We study in detail important statistical properties, such as the density of states, localization length, and the level spacing in percolation composites.
Precision medicine to change the landscape of cardiovascular drug development (2017)

Introduction: Precision cardiovascular medicine, while still in development, holds great promise for the development of more and better therapeutic interventions. The field of cardiovascular disease (CVD) research is starting to embrace novel strategies supported by the abundance of genetic discoveries made in the last decade. Precision medicine has the potential to revitalize the last two decades of lethargy in CVD drug development, which has been declining since the 1990s while CVD remains the leading cause of death worldwide.Areas covered: The authors present recent CVD precision medicine discoveries and discuss the potential for a fundamental shift in the cardiovascular drug development programs.Expert commentary: The dal-GenE trial, with dalcetrapib and its companion diagnostic, has the potential to set the stage for a new era of precision drug development in CVD. Pharmacogenomics could help reduce late-stage failures if implemented early in drug development programs. Stratification of patients can lead to more efficient outcomes trials providing more robust evidence to regulatory agencies.
Adiposity, dyslipidemia, and insulin resistance in mice with targeted deletion of phospholipid scramblase 3 ( PLSCR3 ) (2004)

The phospholipid scramblases (PLSCR1 to PLSCR4) are a structurally and functionally unique class of proteins, which are products of a tetrad of genes conserved from Caenorhabditis elegans to humans. The best characterized member of this family, PLSCR1, is implicated in the remodeling of the transbilayer distribution of plasma membrane phospholipids but is also required for normal signaling through select growth factor receptors. Mice with targeted deletion of PLSCR1 display perinatal granulocytopenia due to defective response of hematopoietic precursors to granulocyte colony-stimulating factor and stem cell factor. To gain insight into the biologic function of another member of the PLSCR family, we investigated mice with targeted deletion of PLSCR3, a protein that like PLSCR1 is expressed in many blood cells but which, by contrast to PLSCR1, is also highly expressed in fat and muscle. PLSCR3(-/-) mice at 2 months of age displayed aberrant accumulation of abdominal fat when maintained on standard rodent chow, which was accompanied by insulin resistance, glucose intolerance, and dyslipidemia. Primary adipocytes and cultured bone-marrow-derived macrophages from PLSCR3(-/-) mice were engorged with neutral lipid, and adipocytes displayed defective responses to exogenous insulin. Plasma of PLSCR3(-/-) mice was elevated in non-high-density lipoproteins, cholesterol, triglycerides, nonesterified fatty acids, and leptin, whereas adiponectin was low. These data suggest that the expression of PLSCR3 may be required for normal adipocyte and/or macrophage maturation or function and raise the possibility that deletions or mutations affecting the PLSCR3(-/-) gene locus may contribute to the risk for lipid-related disorders in humans.
Models to relate wafer geometry measurements to in-plane distortion of wafers (2016)

Achieving satisfactory overlay is increasingly challenging as feature sizes are reduced and allowable overlay budgets shrink to several nanometers and below. Overlay errors induced by wafer processing, such as film deposition and etching, constitute a meaningful fraction of overlay budgets. Wafer geometry measurements provide the opportunity to quantify stress-induced distortions at the wafer level and provide information that can be used in a feedback mode to alter wafer processing or in a feed-forward mode to set wafer-specific corrections in the lithography tool. In order for such feed-forward schemes based on wafer geometry to be realized, there is a need for mechanics models that relate in-plane distortion of a chucked wafer to the out-of-plane distortion of a wafer in a free state. Here, a simple analytical model is presented that shows the stress-induced component of overlay is correlated to a corrected local wafer slope metric for a wide range of cases. The analytical model is validated via finite element (FE) simulations of wafers with nonuniform stress distributions. Furthermore, FE modeling is used here to examine the effect of the spatial wavelength of stress variation on the connection between slope and the wafer stress-induced component of overlay.
In Reply: Re Measurement of Pudendal Evoked Potentials During Feminizing Genitoplasty (1996)

No AccessJournal of UrologyClinical Urology: Letter to the Editor1 Sep 1996In Reply: Re Measurement of Pudendal Evoked Potentials During Feminizing GenitoplastyTechnique and Applications J.P. Gearhart, A. Burnett, and J.H. Owen J.P. GearhartJ.P. Gearhart More articles by this author , A. BurnettA. Burnett More articles by this author , and J.H. OwenJ.H. Owen More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)65737-9AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "In Reply: Re Measurement of Pudendal Evoked Potentials During Feminizing Genitoplasty." The Journal of Urology, 156(3), p. 1140 © 1996 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 156Issue 3September 1996Page: 1140 Advertisement Copyright & Permissions© 1996 by American Urological Association, Inc.MetricsAuthor Information J.P. Gearhart More articles by this author A. Burnett More articles by this author J.H. Owen More articles by this author Expand All Advertisement PDF downloadLoading ...
Identification and Characterization of a Phosphoinositide Phosphate Kinase Homolog (2004)

Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P(2)) plays a central role in regulating the actin cytoskeleton as a substrate for phosphoinositide 3-kinase and phospholipase C as well as by binding directly to proteins that control the processes of actin monomer sequestration, filament severing, capping, nucleation, cross-linking, and bundling (Ma, L., Cantley, L. C., Janmey, P. A., and Kirschner, M. W. (1998) J. Cell Biol. 140, 1125-1136; Hinchliffe, K. (2000) Curr. Biol. 10, R104-R1051). Three related phosphatidylinositol 4-phosphate 5-kinases (PI(4)P 5-kinases) have been identified in mammalian cells (types Ialpha, Ibeta, and Igamma) and appear to play distinct roles in actin remodeling. Here we have identified a fourth member of this family by searching the human genome and EST data bases. This new protein, which we have designated phosphatidylinositol phosphate kinase homolog (PIPKH), is expressed at relatively high levels in brain and testis. Immunoprecipitates of PIPKH expressed in mammalian cells contain PI(4)P 5-kinase activity, but this activity is not affected by mutations in residues that inactivate other type I PI(4)P 5-kinases. We show that the PI(4)P 5-kinase activity in PIPKH immunoprecipitates can be explained by the ability of PIPKH to heterodimerize with other type I PI(4)P 5-kinases. Transfection of 293t cells with PIPKH resulted in >8-fold increase in total phosphatidylinositol 3,4,5-trisphosphate (PI(3,4,5)P(3)) without a significant net increase in total PI(4,5)P(2). When coexpressed with PIPKH, green fluorescent protein (GFP) fusion construct of the pleckstrin homology domain from Bruton's tyrosine kinase (GFP-BTK-PH) localized in intracellular vesicular structures, suggesting an unusual intracellular site of PI(3,4,5)P(3) production. Finally, expression of PIPKH induced the reorganization of actin from predominantly stress fibers to predominantly foci and comets similar to those observed previously in cells infected with the intracellular pathogen Listeria or transfected with recombinant PIPKIalpha. These results suggest that PIPKH acts as a scaffold to localize and regulate type I PI(4)P 5-kinases and the synthesis of PI(3,4,5)P(3).
Immortalization of Human Neural Stem Cells with the c-Myc Mutant T58A (2008)

Human neural stem cells (hNSC) represent an essential source of renewable brain cells for both experimental studies and cell replacement therapies. Their relatively slow rate of proliferation and physiological senescence in culture make their use cumbersome under some experimental and pre-clinical settings. The immortalization of hNSC with the v-myc gene (v-IhNSC) has been shown to generate stem cells endowed with enhanced proliferative capacity, which greatly facilitates the study of hNSCs, both in vitro and in vivo. Despite the excellent safety properties displayed by v-IhNSCs--which do not transform in vitro and are not tumorigenic in vivo--the v-myc gene contains several mutations and recombination elements, whose role(s) and effects remains to be elucidated, yielding unresolved safety concerns. To address this issue, we used a c-myc T58A retroviral vector to establish an immortal cell line (T-IhNSC) from the same hNSCs used to generate the original v-IhNSCs and compared their characteristics with the latter, with hNSC and with hNSC immortalized using c-myc wt (c-IhNSC). T-IhNSCs displayed an enhanced self-renewal ability, with their proliferative capacity and clonogenic potential being remarkably comparable to those of v-IhNSC and higher than wild type hNSCs and c-IhNSCs. Upon growth factors removal, T-IhNSC promptly gave rise to well-differentiated neurons, astrocytes and most importantly, to a heretofore undocumented high percentage of human oligodendrocytes (up to 23%). Persistent growth-factor dependence, steady functional properties, lack of ability to generate colonies in soft-agar colony-forming assay and to establish tumors upon orthotopic transplantation, point to the fact that immortalization by c-myc T58A does not bring about tumorigenicity in hNSCs. Hence, this work describes a novel and continuous cell line of immortalized human multipotent neural stem cells, in which the immortalizing agent is represented by a single gene which, in turn, carries a single and well characterized mutation. From a different perspective, these data report on a safe approach to increase human neural stem cells propagation in culture, without altering their basic properties. These T-IhNSC line provides a versatile model for the elucidation of the mechanisms involved in human neural stem cells expansion and for development of high throughput assays for both basic and translational research on human neural cell development. The improved proclivity of T-IhNSC to generate human oligodendrocytes propose T-IhNSC as a feasible candidate for the design of experimental and, perhaps, therapeutic approaches in demyelinating diseases.
Flexible Molybdenum Electrodes towards Designing Affinity Based Protein Biosensors (2016)

Molybdenum electrode based flexible biosensor on porous polyamide substrates has been fabricated and tested for its functionality as a protein affinity based biosensor. The biosensor performance was evaluated using a key cardiac biomarker; cardiac Troponin-I (cTnI). Molybdenum is a transition metal and demonstrates electrochemical behavior upon interaction with an electrolyte. We have leveraged this property of molybdenum for designing an affinity based biosensor using electrochemical impedance spectroscopy. We have evaluated the feasibility of detection of cTnI in phosphate-buffered saline (PBS) and human serum (HS) by measuring impedance changes over a frequency window from 100 mHz to 1 MHz. Increasing changes to the measured impedance was correlated to the increased dose of cTnI molecules binding to the cTnI antibody functionalized molybdenum surface. We achieved cTnI detection limit of 10 pg/mL in PBS and 1 ng/mL in HS medium. The use of flexible substrates for designing the biosensor demonstrates promise for integration with a large-scale batch manufacturing process.
Chromatin accessibility landscapes activated by cell-surface and intracellular immune receptors (2021)

Activation of cell-surface and intracellular receptor-mediated immunity results in rapid transcriptional reprogramming that underpins disease resistance. However, the mechanisms by which co-activation of both immune systems lead to transcriptional changes are not clear. Here, we combine RNA-seq and ATAC-seq to define changes in gene expression and chromatin accessibility. Activation of cell-surface or intracellular receptor-mediated immunity, or both, increases chromatin accessibility at induced defence genes. Analysis of ATAC-seq and RNA-seq data combined with publicly available information on transcription factor DNA-binding motifs enabled comparison of individual gene regulatory networks activated by cell-surface or intracellular receptor-mediated immunity, or by both. These results and analyses reveal overlapping and conserved transcriptional regulatory mechanisms between the two immune systems.