gebhart/patrepeval huggingface dataset:
Identification and characterization of the G15D mutation found in a male patient with 3.beta.-hydroxysteroid dehydrogenase (3.beta.-HSD) deficiency: alteration of the putative NAD-binding domain of Type II 3.beta.-HSD (1995)

We report the detection of a homozygous G to A mutation converting codon Gly15 into Asp15 in the type II 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase (3 beta-HSD) gene in a male pseudo-hermaphrodite born from consanguineous parents and suffering from severe salt-losing 3 beta-HSD deficiency. To investigate further the potential involvement of residue 15 in the beta alpha beta dinucleotide-binding fold, we have studied the effect of substituting Gly15 for Ala15. We assessed the effect of the G15D and G15A missense mutations on enzymatic activity by analyzing mutant enzymes generated by site-directed mutagenesis of type II 3 beta-HSD cDNA after their transient expression in COS-1 cells. In intact transfected cells, after a 2-h incubation, the percentage of conversion of [3H]pregnenolone (PREG) into [3H]progesterone (PROG) was 35% and 50% for the G15A and native type II 3 beta-HSD enzymes, respectively, whereas no detectable activity was observed in cells expressing the G15D protein. This finding is in agreement with the severity of the disease in the homozygote G15D index case. On the other hand, in homogenates from cells transfected with the normal pCMV-type II 3 beta-HSD plasmid or with the mutated pCMV-G15D or pCMV-G15A plasmid, the Km values for PREG were 0.72 microM, 3.2 microM, and 3.4 microM, respectively, when incubated for 1 h in the presence of excess (1 mM) NAD+. Moreover, the expressed G15D and G15A proteins had decreased affinities for NAD+ with Km values of 113 microM and 148 microM, respectively, compared with 22 microM for normal type II 3 beta-HSD.(ABSTRACT TRUNCATED AT 250 WORDS)
Solution Electrochemiluminescent Microfluidic Cell for Flexible and Stretchable Display (2009)

We developed liquid luminescent flexible displays using electrochemiluminescent solution as deformable luminescent material. The solution consisted of ruthenium complexes as light emitting material and ionic liquid as nonvolatile solvent. We injected the solution, to pattern and seal it, into microfluidic cells between two flexible films of polyethylene terephthalate substrates with indium tin oxide electrodes. The light emitting area of one cell was 2times2 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> . The gap between the electrodes was 100 mum. We measured the luminance of the cell during application of 50 Hz/4.0 Vpp (peak-to-peak value) rectangular waves of voltage in bent condition. The device was bent along the convex curvature with radii of 60, 30 and 20 mm. The luminance was reduced by only 30 % when the radius of cells are suitable for flexible displays because they can tolerate large deformation of bending.
Serum Can Act as a Shear Protecting Agent in Agitated Hybridoma Cell Cultures (1989)

Hybridoma cells, S3H5/gamma 2bA2, were grown in spinner flasks containing RPMI 1640 media at different levels of serum. Cells in low serum media (1% fetal bovine serum) were more sensitive to shear induced by mechanical agitation than cells in high serum media (10%), indicating that serum can protect cells from shear induced by mechanical agitation. The data suggest that serum alters the physiological properties of the cells to make them more shear-resistant, rather than by changing the physico-chemical properties of medium.
Identification of novel therapeutic targets in acute leukemias with NRAS mutations using a pharmacologic approach (2015)

Oncogenic forms of NRAS are frequently associated with hematologic malignancies and other cancers, making them important therapeutic targets. Inhibition of individual downstream effector molecules (eg, RAF kinase) have been complicated by the rapid development of resistance or activation of bypass pathways. For the purpose of identifying novel targets in NRAS-transformed cells, we performed a chemical screen using mutant NRAS transformed Ba/F3 cells to identify compounds with selective cytotoxicity. One of the compounds identified, GNF-7, potently and selectively inhibited NRAS-dependent cells in preclinical models of acute myelogenous leukemia and acute lymphoblastic leukemia. Mechanistic analysis revealed that its effects were mediated in part through combined inhibition of ACK1/AKT and of mitogen-activated protein kinase kinase kinase kinase 2 (germinal center kinase). Similar to genetic synthetic lethal approaches, these results suggest that small molecule screens can be used to identity novel therapeutic targets in cells addicted to RAS oncogenes.
Reducing Effect of a Combination ofPhaseolus vulgarisandCynara scolymusExtracts on Food Intake and Glycemia in Rats (2012)

Extracts from Phaseolus vulgaris and Cynara scolymus may reduce food intake and/or postprandial glycemia. This study investigated the effect of standardized extracts of P. vulgaris and C. scolymus and their combination on food intake and glycemia in rats. P. vulgaris and C. scolymus extracts, and their 1:2 combination, were administered acutely to rats (a) given access to regular food and water, (b) given access to regular food, water, and a chocolate-flavored beverage, or (c) infused with a starch bolus. P. vulgaris extract and the combination produced comparable reductions in intake of regular food and chocolate-flavored beverage; conversely, C. scolymus extract was ineffective on both parameters. P. vulgaris and C. scolymus extracts additively contributed to the reducing effect of the combination on glycemic rise. These results suggest that a mixture of P. vulgaris and C. scolymus extracts is preferable over each single extract, as it combines the anorectic effect of the P. vulgaris extract with the hypoglycemic effect of both extracts. These data support the recent clinical use of the combination of P. vulgaris and C. scolymus extracts in the control of appetite, food intake, and postprandial glycemia and represent a successful example of translational research in the nutraceutical field.
Classifying convex sets for vessel detection in retinal images (2003)

We present a method to detect vessels in images of the retina. Instead of relying on pixel classification, as many detection algorithms do, we propose a more natural representation for elongated structures, such as vessels. This new representation consists of primitives called affine convex sets. On these convex sets we apply the classification step. The reason for choosing this approach is two-fold: (1) By using a dedicated representation of image structures, one can exploit prior knowledge. (2) A method based on pixel classification is often computationally unattractive. The method can also be applied to other image structures, if an appropriate representation for the structures is chosen. The method was tested on fundus reflection images. We obtained an accuracy of 0.897, a sensitivity of 0.700 and a specificity of 0.923.
Multivalley Electron Conduction at the Indirect-Direct Crossover Point in Highly Tensile-Strained Germanium (2022)

For applications such as low-power tunneling transistors and memory, lasers, qubits, and spintronics, there is interest in using strain to enhance carrier mobility in semiconductors. This experimental and theoretical study investigates transport in highly strained germanium, in which multivalley electron conduction emerges. Surprisingly, all conduction occurs via the L valley in Ge for biaxial tensile strain below 1.6%, while above the contribution from the \ensuremath{\Gamma} valley increases, with negligible contributions from the underlying strain template.
Characterization of Morreton Virus (MORV) as a Novel Oncolytic Virotherapy Platform for Liver Cancers (2022)

Abstract Morreton virus (MORV) is a novel oncolytic Vesiculovirus , genetically distinct from vesicular stomatitis virus (VSV). we report that MORV induced potent cytopathic effects in a panel of cholangiocarcinoma (CCA) and hepatocellular carcinoma (HCC) cell lines. In addition, high intranasal doses of MORV were not associated with significant adverse effects and were well tolerated in mice bearing liver tumor xenografts and syngeneic liver cancers. Furthermore, single intratumoral treatments with MORV (1 x 10 7 TCID 50 ) triggered a robust antitumor immune response leading to substantial tumor regression and disease control in a syngeneic CCA model, using 10-fold lower dose compared to VSV (1 x 10 8 TCID 50 ). In addition, MORV and VSV both induced prominent tumor growth delay in immunodeficient mice bearing Hep3B hepatocellular carcinoma (HCC) but not in mice bearing HuCCT-1 CCA xenografts. Our findings indicate that wild-type MORV is safe and can induce potent tumor regression in HCC and CCA animal models without adverse events via immune-mediated and immune-independent mechanisms. Further development and clinical translation of MORV as virotherapy for liver cancers are warranted.
43.3: How Fill Factor Affects Display Image Quality (1998)

Abstract A psychophysical experiment was conducted to investigate the effect of field emission display (FED) fill factor on display image quality. A two‐alternative, forced‐choice psychophysical task was employed to obtain judgments of image coarseness and overall image quality as a function of three fill factors, 30 percent, 40 percent and 69 percent of VGA resolution FEDs. The results revealed that FED imaging performance is not improved by increasing the fill factor of 325 micron pixel pitch FEDs, provided that space‐average luminance is maintained constant.
Electrostatics of Particle Confinement within Nanochannels: Role of Double-Layer Interactions and Ion-Ion Correlations (2017)

We investigate electrolyte-mediated interactions of charged analyte particles confined within nanochannels. We investigate how the free energy of analyte particle position varies with ionic strength and ion valence for multivalent 2:1 electrolytes and monovalent 1:1 electrolytes. Our studies use a particle-based Restrictive Primitive Model (RPM), classical Density Functional Theory (cDFT), and mean-field Poisson-Boltzmann Theory (PB). We assess for the nanochannel system the modeling fidelity of cDFT and PB relative to RPM. We find for sufficiently charged regimes that interesting features in the free energy profile can develop with energy barriers and a minimum giving a preferred localization of a charged analyte particle near the walls of the nanochannel. We discuss the underlying origins of these behaviors by statistically characterizing the contributions of overcharging, overlap of ionic double-layers, and ion-ion correlations. We also discuss how our results may be used to further improve models of confined electrolytes and more generally their implications for phenomena in nanoscale devices.
Ionizing and optical radiation-induced degradation of erbium-doped-fiber amplifiers (1993)

An important aspect of the reliability of an erbium-doped-fiber amplifier is the loss induced by exposure to both high-energy and optical irradiation. It is expected that erbium-doped fiber, which generally contains germanium and aluminum co-dopants, may be more susceptible to radiation-induced darkening than is transmission fiber. We exposed several erbium-doped fibers to optical intensities that are high compared to normal operating conditions. In addition enhanced 7-ray doses were applied. A variety of fiber types were examined; their core compositions were near 15 mol. % Ge and 2-6 mol. % Al, resulting in corecladding index difference An = 0.02 to 0.03.
Gene Assembly from Chip‐Synthesized Oligonucleotides (2012)

<i>De novo</i> synthesis of long double-stranded DNA constructs has a myriad of applications in biology and biological engineering. However, its widespread adoption has been hindered by high costs. Cost can be significantly reduced by using oligonucleotides synthesized on high-density DNA chips. However, most methods for using off-chip DNA for gene synthesis have failed to scale due to the high error rates, low yields, and high chemical complexity of the chip-synthesized oligonucleotides. We have recently demonstrated that some commercial DNA chip manufacturers have improved error rates, and that the issues of chemical complexity and low yields can be solved by using barcoded primers to accurately and efficiently amplify subpools of oligonucleotides. This article includes protocols for computationally designing the DNA chip, amplifying the oligonucleotide subpools, and assembling 500-800 basepair (bp) constructs.
Electrodeposited polyethylenedioxythiophene with infiltrated gel electrolyte interface: a close contest of an all-solid-state supercapacitor with its liquid-state counterpart (2014)

We report the design of an all-solid-state supercapacitor, which has charge storage characteristics closely matching that of its liquid-state counterpart even under extreme temperature and humidity conditions. The prototype is made by electro-depositing polyethylenedioxythiophene (PEDOT) onto the individual carbon fibers of a porous carbon substrate followed by intercalating the matrix with polyvinyl alcohol-sulphuric acid (PVA-H2SO4) gel electrolyte. The electrodeposited layer of PEDOT maintained a flower-like growth pattern along the threads of each carbon fiber. This morphology and the alignment of PEDOT led to an enhanced surface area and electrical conductivity, and the pores in the system enabled effective intercalation of the polymer-gel electrolyte. Thus, the established electrode-electrolyte interface nearly mimics that of its counterpart based on the liquid electrolyte. Consequently, the solid device attained very low internal resistance (1.1 Ω cm(-2)) and a high specific capacitance (181 F g(-1)) for PEDOT at a discharge current density of 0.5 A g(-1). Even with a high areal capacitance of 836 mF cm(-2) and volumetric capacitance of 28 F cm(-3), the solid device retained a mass-specific capacitance of 111 F g(-1) for PEDOT. This is in close agreement with the value displayed by the corresponding liquid-state system (112 F g(-1)), which was fabricated by replacing the gel electrolyte with 0.5 M H2SO4. The device also showed excellent charge-discharge stability for 12 000 cycles at 5 A g(-1). The performance of the device was consistent even under wide-ranging humidity (30-80%) and temperature (-10 to 80 °C) conditions. Finally, a device fabricated by increasing the electrode area four times was used to light an LED, which validated the scalability of the process.
Increased and balanced light emission of transparent organic light-emitting diodes by enhanced microcavity effects (2011)

We report on improved and controlled light outcoupling of transparent organic light-emitting diodes (TOLEDs) by inserting thin silver layers between the indium tin oxide anode and the hole transporting layer. The introduction of Ag layers influences both the bottom and top emission of the TOLEDs, and it results in dramatic changes in the electroluminescence spectra and angular distribution. We find that the overall external quantum efficiency can be increased up to 18.8%, and the ratio of bottom and top emission can be almost identical.
Intramolecular nitrile oxide cycloaddition route to carbocyclics: a formal total synthesis of PGF2.alpha. (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIntramolecular nitrile oxide cycloaddition route to carbocyclics: a formal total synthesis of PGF2.alpha.Alan P. Kozikowski and Philip D. SteinCite this: J. Org. Chem. 1984, 49, 13, 2301–2309Publication Date (Print):June 1, 1984Publication History Published online1 May 2002Published inissue 1 June 1984https://pubs.acs.org/doi/10.1021/jo00187a001https://doi.org/10.1021/jo00187a001research-articleACS PublicationsRequest reuse permissionsArticle Views1082Altmetric-Citations68LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
NMR Structural Characterization of Peptide Inhibitors Bound to the Hepatitis C Virus NS3 Protease:  Design of a New P2 Substituent (2003)

A comparative NMR conformational analysis of three distinct tetrapeptide inhibitors of the Hepatitis C NS3 protease that differ at the 4-aryloxy-substituted P2 proline position was undertaken. Specifically, transferred nuclear Overhauser effect experiments in combination with restrained systematic conformational searches were used to characterize the orientation of the P2 aryl substituents of these inhibitors when bound to the NS3 protease. Differences between free and bound conformations were also investigated. Analysis of the results allowed the design of a new P2 aromatic substituent, which significantly increased the potency of our inhibitors. The bound conformation of a specific competitive inhibitor having this novel P2 substituent is also described, along with a model of this inhibitor bound to the NS3 protease. This NS3 protease/inhibitor complex model also supports a hypothetical stabilization role for the P2 residue of the substrates and/or inhibitors and further elucidates the subtle details of the binding of the P2 residue of substrate-based inhibitors.
High nuclear poly‐(ADP‐ribose)‐polymerase expression is prognostic of improved survival in pancreatic cancer (2012)

Our analysis shows that nuclear PARP is an independent prognostic marker with respect to standard clinicopathological parameters. These results suggest that PARP should be further explored as a predictive factor with respect to conventional chemotherapy and concepts of PARP inhibitor therapy in pancreatic cancer.
Modification of Pulsed Electric Field Conditions Results in Distinct Activation Profiles of Platelet-Rich Plasma (2016)

PEF activation of PRP at bipolar low vs. monopolar high field strength results in differential platelet-derived microparticle production and activation of platelet surface procoagulant markers while inducing similar release of growth factors and similar capacity to induce cell proliferation. Stimulation of PRP with SMLEF bipolar pulses is gentler than SMHEF pulses, resulting in less platelet microparticle generation but with overall activation levels similar to that obtained with thrombin. These results suggest that PEF provides the means to alter, in a controlled fashion, PRP properties thereby enabling evaluation of their effects on wound healing and clinical outcomes.
Cover Picture: Synthesis and Thermal Behavior of a Fused, Tricyclic 1,2,3,4‐Tetrazine Ring System (Angew. Chem. Int. Ed. 44/2015) (2015)

An energetic fused heterocycle was synthesized in a two-step process described by D. E. Chavez and co-workers in their Communication on page 12973 ff. Firstly, a double N-amination of the bis(tetraethylammonium) salt of 3,3'-dinitro-5,5'-bi-1,2,4-triazole provides a thermally stable, di-N-aminated energetic material that is insensitive to impact, spark, and friction. A subsequent azo coupling reaction produces a fused, tricyclic 1,2,3,4-tetrazine ring system with excellent density and explosive performance properties. An energetic fused heterocycle was synthesized in a two-step process described by D. E. Chavez and co-workers in their Communication on page 12973 ff. Firstly, a double N-amination of the bis(tetraethylammonium) salt of 3,3'-dinitro-5,5'-bi-1,2,4-triazole provides a thermally stable, di-N-aminated energetic material that is insensitive to impact, spark, and friction. A subsequent azo coupling reaction produces a fused, tricyclic 1,2,3,4-tetrazine ring system with excellent density and explosive performance properties. Lithium–Sulfur Batteries 2D Materials Photocatalysts
Specialty fibers shine as high-power, high-beam-quality fiber sources (2007)

For tight focusing or long-distance reach high-power fiber lasers require improved beam quality. Specialty-fiber techniques such as coiling, acoustic guiding layers, and tailored gain and index profiles are lighting -the way.
Conjugate addition of acylate-nickel complexes to quinone monoketals: formal synthesis of the naphthoquinone antibiotics nanaomycin A and deoxyfrenolicin (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTConjugate addition of acylate-nickel complexes to quinone monoketals: formal synthesis of the naphthoquinone antibiotics nanaomycin A and deoxyfrenolicinM. F. Semmelhack, L. Keller, T. Sato, E. J. Spiess, and W. WulffCite this: J. Org. Chem. 1985, 50, 26, 5566–5574Publication Date (Print):December 1, 1985Publication History Published online1 May 2002Published inissue 1 December 1985https://pubs.acs.org/doi/10.1021/jo00350a027https://doi.org/10.1021/jo00350a027research-articleACS PublicationsRequest reuse permissionsArticle Views836Altmetric-Citations32LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Ester type banana-shaped liquid crystalline monomers: synthesis and physical properties (2004)

New ester-type banana (bent-shaped) monomers, 1,3-phenylene bis[4′-(alken-1-yloxy)-biphenyl-4-carboxylate]s Ia–Vb, with different substituent(s) on the central phenyl ring (H, CH3, Cl or NO2) and alkenyl tails in the side arms (decenyl or undecenyl) were prepared. The analogues IIIa–IVb with 4-chloro- or 4,6-dichloro-substituents exhibited a nematic phase, while Va,Vb with 2-nitro-substituent showed a B7 phase at relatively low temperature. All the compounds were stable, no degradation or polymerization was observed under applied electric fields or heat treatments. Electro-hydrodynamic instabilities were observed in the nematic phase of each sample. In the B7 phase of Vb there was an electro-optical switching in the range 112–114.5 °C with a switching time of about 150 µs at 20 V µm−1 field. At lower temperatures no electro-optical switching occurred, but a ferroelectric-type spontaneous polarization of 22 nC cm−2 could be detected. The bend angle was calculated by the semiempirical CAChe/MOPAC/PM5 method.