gebhart/patrepeval huggingface dataset:
Enhanced immunotherapeutic effect of B cell based vaccine transduced with modified adenoviral vector (P4443) (2013)

Abstract For successful clinical outcome of tumor immunotherapy, proper generation of strong immune responses against tumor antigen is required. Cell based vaccine is one of the strategy to induce appropriate immune responses. In previous studies we established NKT ligand loaded, adeoviral antigen transduced-B cell based anticancer cellular vaccine that efficiently induced a wide spectrum of immune responses against tumor antigen. However the lack of the coxsakivirus-adenovirus receptor(CAR) on B cells limits the adenoviral Ag gene delivery. In this study, we established a modified adenoviral vector(Ad-k35) whose fiber structure was substituted with adenovirus serotype 35, encoding truncated breast cancer antigen Her2/neu(Ad-k35HM), to enhance tumor antigen gene delivery to B cells. Using Ad-k35HM, we observed increased expression of tumor antigen on both human and mouse B cells. And Ad-k35HM transduced B cell vaccine elicited antigen specific strong cellular and humoral immune responses and these responses was further enhanced with additional loading of derivative of NKT ligand KBC009. Ad-k35HM transduced, KBC009 loaded B cell vaccination efficiently suppressed in vivo established tumor growth in a mice model. These findings showed modified adenoviral vector Ad-k35 could be appropriate for the preclinical and clinical development of B cell based anticancer immunotherapy.
ChemInform Abstract: CHEMIE DER ALPHA‐ALKOXY‐SULFOXIDE, BLDG. VON METHYLENACETALEN AUS DIMETHYLSULFOXID UND ALKOHOLEN (1973)

Abstract In einer Lösung von N‐Brom‐succinimid und Dimethylsulfoxid bilden die Alkohole (I) nach mehrstündigem Rühren bei 50°C die Acetale (II).
Region-level Tracking for Scalable Directory Cache (2015)

Traditional coherence directories track sharing information at a cache-line granularity. In practice, however, as data sharing occurs at a coarser granularity in a large region of memory, common sharing patterns tend to be observed across multiple proximate lines. Hence, the directory entries for the lines replicate the same sharing information, resulting in inefficient use of space, power, and energy.&#13;\nIn this paper, we empirically demonstrate "region-level sharing pattern locality", that is, a small number of distinct sharing patterns are observed across proximate lines within a large region of memory, e.g., a page unit. We leverage this phenomenon to propose a new representation of sharing information, called Region-level Sharing information Tracking (RST), that dynamically maintains common sharing information in a space-efficient manner at a region-level. Our experimental results based on conventional parallel and server workloads show that RST reduces over 75% of the area (and hence energy) compared to conventional directory caches, with almost negligible performance overhead.
Assessment Of The Utility Of Subset Donor Chimerism For Predicting Event-Free Survival After Non-Myeloablative Allogeneic Hematopoietic Cell Transplantation Using TLI And ATG (2010)

Allogeneic hematopoietic cell transplantation (HCT) following conditioning with total lymphoid irradiation (TLI) and antithymocyte globulin (ATG) is an effective treatment modality for leukemia and lymphoma. We recently reported 3-year event-free survival rates for AML and NHL of 40% and 42%, respectively (Kohrt et al., Blood, 114:1099-1109). Disease relapse remains the primary cause of treatment failure, thus the ability to predict when patients are at risk for relapse would allow for timely immunologic interventions, such as alterations of immunosuppressive therapy or donor lymphocyte infusions. We analyzed CD3/15/19/56 peripheral blood chimerism in 122 patients who underwent allo-HCT after TLI-ATG conditioning between December 2001 and July 2009. Univariate analysis comparing complete chimerism (>95% donor CD3) to mixed chimerism (5-95% donor CD3) did not reliably predict events (relapse or death). Several methods of evaluating the predictive value of subset chimerism were then assessed, including Cox regression analysis of chimerism trajectory, receiver operator characteristic (ROC) analysis of chimerism trajectory, and ROC analysis of individual chimerism measurements at days 90, 120, 150, and 180 after HCT. Although subset chimerism generally performed poorly as a predictor of outcome with all analytic strategies, ROC analysis of chimerism trajectory and individual measurements demonstrated limited utility for predicting event-free survival. We calculated the area under the ROC curve (AUC), which represents the probability that a randomly chosen patient with an observed event has lower chimerism than a randomly chosen patient without an observed event. An AUC greater than 80% represents a high likelihood of event prediction. For patients with lymphoid malignancies, all subsets exhibited poor predictive value using all methods, and with ROC analysis the AUC was uniformly less than 70% for all subset-timepoint combinations. In patients with myeloid malignancies, only CD15 chimerism was predictive of event-free survival, with AUC values of 84% at day 90, 79% at day 120, 82% at day 150 and 88% at day 180. We conclude that subset donor chimerism has little utility for predicting event-free survival in lymphoid malignancies, and that only CD15 chimerism predicts outcome in myeloid malignancies. Additional patient characteristics will need to be incorporated into the decision to apply immunological therapies to alter outcome after non-myeloablative HCT.
Unbound bilirubin levels in phototherapy‐treated preterm infants receiving soy‐based lipid emulsion (2020)

Bf and TSB responded differently. While TSB and Bf correlated well before phototherapy, they did not correlate during phototherapy. TSB showed a trend toward a reduction with treatment, Bf did not. While TSB ROR information is not helpful, ROR Bf data can be utilized to anticipate treatment. Potentially high Bf levels existed before and after phototherapy and the mean Bf level at phototherapy termination remained elevated in a significant proportion of infants.
Unraveling the Prenylation–Cancer Paradox in Multiple Myeloma with Novel Geranylgeranyl Pyrophosphate Synthase (GGPPS) Inhibitors (2018)

Post-translational prenylation of the small GTP-binding proteins (GTPases) is vital to a plethora of biological processes, including cellular proliferation. We have identified a new class of thienopyrimidine-based bisphosphonate (ThP-BP) inhibitors of the human geranylgeranyl pyrophosphate synthase (hGGPPS) that block protein prenylation in multiple myeloma (MM) cells leading to cellular apoptosis. These inhibitors are also effective in blocking the proliferation of other types of cancer cells. We confirmed intracellular target engagement, demonstrated the mechanism of action leading to apoptosis, and determined a direct correlation between apoptosis and intracellular inhibition of hGGPPS. Administration of a ThP-BP inhibitor to a MM mouse model confirmed in vivo downregulation of Rap1A geranylgeranylation and reduction of monoclonal immunoglobulins (M-protein, a biomarker of disease burden) in the serum. These results provide the first proof-of-principle that hGGPPS is a valuable therapeutic target in oncology and more specifically for the treatment of multiple myeloma.
Small Molecule Inhibitors of EGFR Ectodomain for Breast Cancer Therapy (2009)

Abstract : The goal of this proposal was to develop low molecular weight inhibitors of EGFR that disable receptor functioning by preventing critical activating transitions of the extracellular domain. The lead compound, EL1-FD1, has been designed and found able to reverse the malignant properties of EGFR transformed cells in vitro and in vivo. During the reporting period, the designed EGFR ectodomain inhibitor has been tested for inhibition of EGFR phosphorylation and growth of breast cancer cells in vitro and in vivo. The compound has been shown to inhibit EGF induced phosphorylation of EGFR in EGFR overexpressing cells. A strong inhibitory effect of the compound against MDA-MB-468 cells has been demonstrated in a poly-HEMA assay. The compound effectively inhibited tumor growth in mice with MDA-MB-468 and A431 xenografts and had a moderate effect in MDA-MB-231 xenografts in vivo. Crystallization studies have produced two types of EGFR crystals suitable for soaking experiments with the inhibitory compounds. A number of structural analogs have been designed that have similar antitumor activities, but more favorable pharmacological properties, compared to the lead compound.
A Radical Cascade Cyclization To Prepare Dihydrothiophenes Induced by Thiyl Radicals as Sulfur Biradical Equivalents (2013)

Bicyclic dihydrothiophenes are readily prepared by a radical cascade cyclization reaction triggered by the addition of a thiyl radical under thermal or photoirradiation conditions. The translocated radical attacks the sulfur atom in the initial radical donor unit in an SHi manner. Sufficient stereoselectivity is achieved when a large excess of disulfide is used for the reaction under photoirradiation conditions. The reaction in the absence of solvents provides vinylsulfides instead of dihydrothiophenes. Thus, the sulfur atom in the thiyl radical serves as a sulfur biradical synthetic equivalent.
Regional Restriction of Alcohol/Retinol Dehydrogenases along the Mouse Gastrointestinal Epithelium (1997)

The gastrointestinal tract is a major site of alcohol dehydrogenase (ADH) activity in humans and rodents. Because class I ADH (ADH-I) and class IV ADH (ADH-IV), but not class III ADH (ADH-III), function as retinol dehydrogenases in vitro and may thus participate in retinoid signaling needed for epithelial differentiation, the aim of this study was to determine the localization of these enzymes along the gastrointestinal tract. Specific antibodies were used to examine the tissue distribution of all three known classes of mouse ADH by Western blotting, and cellular localization was determined by immunohistochemistry. ADH-I was detected primarily in the intestine, liver, kidney, adrenal, and uterus, with detection of ADH-III in all tissues examined, and detection of ADH-IV primarily in the esophagus, stomach, adrenal, skin, ovary, and epididymis. Along the gastrointestinal tract, ADH-III was not specifically localized, whereas ADH-I was localized exclusively in the villus epithelium of the small intestine and absorptive epithelium of the large intestine, with ADH-IV being localized exclusively in the basal and suprabasal epithelial cells of the esophagus and gastric pit surface epithelium of the stomach. The ADH localization patterns observed are consistent with ADH-I and ADH-IV, but not ADH-III, functioning physiologically in retinol metabolism needed for epithelial differentiation. Our results further suggest that the functions of ADH-I and ADH-IV are regionally restricted to the lower and upper components, respectively, of the gastrointestinal epithelium, a finding that may relate to the different efficiencies of these two enzymes for retinol oxidation, as well as to the different susceptibilities of the upper and lower digestive tracts for ethanol-induced cancers.
Development and Optimization of Intermediate Lock Position Camshaft Phaser System (2010)

&lt;div class="section abstract"&gt;&lt;div class="htmlview paragraph"&gt;Intake camshaft retard beyond that necessary for reliable cold start-ability is shown to improve part-load fuel economy. By retarding the intake camshaft timing, engine pumping losses can be reduced and fuel economy significantly improved. At high engine speeds, additional intake cam retard may also improve full-load torque and power.&lt;/div&gt;&lt;div class="htmlview paragraph"&gt;To achieve these benefits, an intake camshaft phaser with intermediate lock pin position (ILP) and increased phaser authority was developed. ILP is necessary to reliably start at the intermediate phase position for cold temperatures, while providing increased phaser retard under warm conditions. The phaser also provides sufficient intake advance to maximize low-speed torque and provides good scavenging for boosted engine applications.&lt;/div&gt;&lt;div class="htmlview paragraph"&gt;Design and development of the intermediate locking phaser system is described. The pros and cons of various methods of accomplishing locking and unlocking a phaser are illustrated. An AMESim model of the phaser was developed and Robust Engineering methods were applied.&lt;/div&gt;&lt;div class="htmlview paragraph"&gt;Engine simulation and dynamometer testing was performed to determine system benefits. Control algorithms were developed and an enhanced engine management system was demonstrated in a vehicle.&lt;/div&gt;&lt;/div&gt;
Intracellular Delivery of Functional Proteins (2022)

Leveraging proteins as therapeutics has revolutionized the way we treat diseases. Recent advances in protein biologics and antibody engineering represent promising new treatment strategies for addressing complex etiologies. Thus far, these advances have been restricted to only accessible extracellular proteins, leaving a significant portion of the proteome undruggable. Efficient intracellular delivery of proteins would reveal uncharted therapeutic avenues and cell surface engineering opportunities. This presentation describes combinatorial strategies towards delivering proteins to the cytosol. By modifying a protein of interest through surface esterification or functionalization with a cell penetrating peptide we have observed robust intracellular delivery. In this presentation, I will first describe the development of a functional assay using a model protein to assess the efficacy and localization of delivery agents. Applying these findings to HaloTag has demonstrated the generality of our approach. I will show that our delivery strategy has enabled stable cell surface engineering. This study highlights that multiple intracellular delivery strategies can be used synergistically to enable cytosolic access with functionally active protein/biologic cargo.
Electromagnetic Interactions in Plasmonic Nanoparticle Arrays (2005)

Single two-dimensional planar silver arrays and one-dimensional linear gold chains of nanoparticles were investigated by dark-field surface plasmon spectroscopy and studied as a function of interparticle distance, particle size, and number of particles. In agreement with recent theoretical predictions, a red shift of the surface plasmon resonance occurring in two-dimensional arrays was found for lattice spacings below 200 nm. This red shift is associated with a significant broadening of the resonance and is attributed to the onset of near-field interactions. We found that the relative contributions of the long-range and short-range interactions in two-dimensional arrays of particles are fundamentally different to those occurring in individual linear chains.
Autocalibration and compensation techniques (2006)

The design of analog circuits is rendered difficult by the imperfections imparted by the manufacturing process to the component values. Physical parameters (e.g. oxide thickness, physical dimensions, doping profile) are subject to variations due to instabilities of the fabrication technology, and they reflect on component parameters. The best achievable tolerance of individual component values thus depends on the accuracy of the manufacturing process, and cannot be reduced below a minimum level.
Effect of droplet size, droplet placement, and gas dissolution on throughput and defect rate in UV nanoimprint lithography (2016)

Simulation of multidrop spreading in ultraviolet nanoimprint lithography is performed to study the effects of droplet size, droplet arrangement, droplet placement error, and gas diffusion on filling-time and defects. Simulations are carried out for square, hexagonal, and modified hexagonal arrangements of up to 1024 droplets ink-jetted on a substrate to determine the optimum arrangement for minimum imprint time. The effect of error in droplet placement by the inkjet dispenser on the imprint time for different droplet sizes is also investigated. The square droplet arrangement leads to the shortest fluid filling time for a flat template. The filling time increases significantly for droplet placement errors of more than 0.01% relative to the width of the substrate. A model is presented to study the diffusion of gas encapsulated between droplets into the resist. A dimensionless parameter α ∼μD/kHγHo measures the relative importance gas diffusion to hydrodynamics, where D is the gas diffusion constant, kH is the Henry's law constant, μ is the resist viscosity, and γ is the surface tension of the imprint resist. For small values of α, gas diffusion is slower than resist spreading and defect size is diffusion-controlled while for larger values, gas diffusion is faster than resist spreading and the defect size is hydrodynamically controlled. Scaling laws are developed to extrapolate predictions on filling time, residual layer thickness, and defects here for hundreds to a thousand droplets to tens and hundreds of thousands of droplets.
Reinterpretations of an experiment on the backaction in a weak measurement (2013)

Interpretations of an experiment on the backaction in a weak measurement in [Iinuma et al., New J. Phys. 13, 033041 (2011)] are revisited. We show two different but essentially equivalent interpretations for this experiment along the original scenario of weak measurements proposed by Aharonov, Albert, and Vaidman [Phys. Rev. Lett. 60, 1351 (1988)]. To do this, we introduce the notion of extended weak values, which is associated not only with the states of the system but also the state of the measuring device. We also evaluate fluctuations in this experiment, and found that an optimal measurement strength exists for a fixed polarization angle prepared as an initial state, at which fluctuations in measurement results vanish. The consistency of this evaluation with the experimental results is discussed.
Education in Zimbabwe: A Bibliography of Some Major Recent Publications, Reports, and other References (1981)

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Molecular Interaction Fields Edited by Gabriele Cruciani. Wiley-VCH Verlag, Weinheim, Germany. 2006. xviii + 307 pp. 17.5 × 25.5 cm. ISBN 3527310873. $185.00. (2006)

ADVERTISEMENT RETURN TO ISSUEPREVBook ReviewMolecular Interaction Fields Edited by Gabriele Cruciani. Wiley-VCH Verlag, Weinheim, Germany. 2006. xviii + 307 pp. 17.5 × 25.5 cm. ISBN 3527310873. $185.00.Jan W. F. WasleyView Author Information Simpharma LLC 1 Stone Fence Lane Guilford, Connecticut 06437Cite this: J. Med. Chem. 2006, 49, 10, 3032Publication Date (Web):April 25, 2006Publication History Published online25 April 2006Published inissue 1 May 2006https://pubs.acs.org/doi/10.1021/jm0680169https://doi.org/10.1021/jm0680169book-reviewACS PublicationsCopyright © 2006 American Chemical SocietyRequest reuse permissionsArticle Views62Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Drug delivery,Drug discovery,Molecular interactions,Pharmacophores,Software Get e-Alerts
The Next Generation BLAST Experiment (2014)

The Balloon-borne Large Aperture Submillimeter Telescope for Polarimetry (BLASTPol) was a suborbital experiment designed to map magnetic fields in order to study their role in star formation processes. BLASTPol made detailed polarization maps of a number of molecular clouds during its successful flights from Antarctica in 2010 and 2012. We present the next-generation BLASTPol instrument (BLAST-TNG) that will build off the success of the previous experiment and continue its role as a unique instrument and a test bed for new technologies. With a 16-fold increase in mapping speed, BLAST-TNG will make larger and deeper maps. Major improvements include a 2.5-m carbon fiber mirror that is 40% wider than the BLASTPol mirror and ~3000 polarization sensitive detectors. BLAST-TNG will observe in three bands at 250, 350, and 500 μm. The telescope will serve as a pathfinder project for microwave kinetic inductance detector (MKID) technology, as applied to feedhorn-coupled submillimeter detector arrays. The liquid helium cooled cryostat will have a 28-day hold time and will utilize a closed-cycle 3 He refrigerator to cool the detector arrays to 270 mK. This will enable a detailed mapping of more targets with higher polarization resolution than any other submillimeter experiment to date. BLAST-TNG will also be the first balloon-borne telescope to offer shared risk observing time to the community. This paper outlines the motivation for the project and the instrumental design.
A study on the use of phenoxy resins as compatibilizers of polyamide 6 (PA6) and polybutylene terephthalate (PBT) (2003)

Abstract In this study we investigated the potential of phenoxy resins as compatibilizers in the blending of two high‐volume engineering thermoplastics—polyamide 6 (PA6) and polybutylene terephthalate (PBT), in an effort to establish the usefulness of blending as a method of recycling of mixed plastic wastes. It was found that phenoxy resins formed miscible blends with PBT, formed grafted copolymers with PBT through ester exchange reactions, and—though formed immiscible blends with PA6—produced energetic interactions in the form of hydrogen bonding with PA6. The ternary blend systems of 70 parts PA6, 30 parts PBT, and respectively 5, 10, and 30 parts phenoxy resins, all by weight, revealed at two‐phase nature—PA6 as the continuous phase and miscible blends of PBT and phenoxy resins as the dispersed phase—and were found to be stable to phase coarsening by annealing with mechanical properties at least as good as those of the component polymers.