1760 ENDOTHELIAL PROGENITOR CELLS AND VEGF INCREASE NEOVASCULARIZATION AND VOLUME PRESERVATION OF ENGINEERED MUSCLE TISSUES IN VIVO (2010)

You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Incontinence - Evaluation & Therapy III1 Apr 20101760 ENDOTHELIAL PROGENITOR CELLS AND VEGF INCREASE NEOVASCULARIZATION AND VOLUME PRESERVATION OF ENGINEERED MUSCLE TISSUES IN VIVO Zhan Wang, Manish Patel, Tracy Criswell, Dawn Delo-O'Riley, Daniel Eberli, Anthony Atala, and Shay Soker Zhan WangZhan Wang , Manish PatelManish Patel , Tracy CriswellTracy Criswell , Dawn Delo-O'RileyDawn Delo-O'Riley , Daniel EberliDaniel Eberli , Anthony AtalaAnthony Atala , and Shay SokerShay Soker View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.1670AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Stress urinary incontinence (SUI), characterized by involuntary leakage of urine on exertion, sneezing or coughing, is a common condition affecting up to half of women with UI. Studies have shown that muscle progenitor cells (MPCs) injected into the rhabdosphincter survive and differentiate into regenerative myofibers. However, an adequate vascular supply is an important barrier to large muscle tissue regeneration for clinical application. MPCs at the center of an injected cell mass depend on an extensive blood vessel network, which supplies nutrients and oxygen while removing waste products. The objective of this study was to explore the effects neovascularisation on the viability of regenerated striated muscle tissue in vivo, as its utility for the treatment of SUI. To this end, we combined the use of vascular endothelial growth factor (VEGF) gene delivery and implantation of hES-derived endothelial progenitor cells (hESEPCs) to enhance the neovascularization of engineered muscle tissues. METHODS Skeletal muscle fibers from adult mice were harvested from quadriceps, biceps femoris and arm-biceps and plated in Matrigel dishes. Myoblasts migrating from the myofibers were cultured and infected with an adenovirus encoding for VEGF. hES-EPCs were labeled with green fluorescent protein (GFP) for in vivo tracing. Myoblasts and hES-EPC were suspended in collagen type I and injected subcutaneously in nude mice. The engineered tissues were surgically recovered from the injection sites after 4 weeks and underwent anatomical and histological analysis. RESULTS Myoblasts were cultured for 3 passages, expressing skeletal muscle markers and forming beating myofibers. The VEGF-adenovirus transfected myoblasts showed VEGF secretion in vitro. Injected MPCs formed muscle like tissue, as evidenced by H&E staining. Neovascularization was detected in the regenerated muscle tissues of VEGF-infected cells and hES-EPC were shown to participate in blood vessel formation. The VEGF-expressing MPCs co-injected with hES-EPC maintained larger volumes for 4 weeks compared to VEGF-expression alone, whereas the non VEGF-expressing MPCs-derived tissues showed an almost complete volume loss after 10 days. CONCLUSIONS These results indicate that a combination of VEGF and endothelial cells are useful for inducing muscle tissue formation, neovascularization and volume preservation in regenerated muscle tissues and this technique may be useful to enhance the outcomes of muscle cell injection therapy for SUI. Winston-Salem, NC© 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e680-e681 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.Metrics Author Information Zhan Wang More articles by this author Manish Patel More articles by this author Tracy Criswell More articles by this author Dawn Delo-O'Riley More articles by this author Daniel Eberli More articles by this author Anthony Atala More articles by this author Shay Soker More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...