gebhart/patrepeval huggingface dataset:
Blockade of the brain histamine H3 receptor by JNJ-39220675: preclinical PET studies with [11C]GSK189254 in anesthetized baboon (2012)

The robust blockade of binding of [(11)C]GSK189254 by JNJ-39220675 demonstrates that this compound readily penetrates the blood-brain barrier and occupies the histamine H(3) receptor after oral administration at low plasma concentrations (∼1 ng/cc) supporting further drug development for alcohol addiction and other disorders. This study corroborates prior reports of the high sensitivity of [(11)C]GSK189254 to injected mass at doses >0.1 μg/kg.
Palladium nanoparticles stabilised on PEG-modified silica as catalysts for the aerobic alcohol oxidation in supercritical carbon dioxide (2006)

PEG-modified (PEG = polyethylene glycol) silica surfaces are able to effectively stabilize and immobilize palladium nanoparticles for their use as selective oxidation catalysts in combination with scCO2 as reaction medium under mild conditions. These catalysts show high activity and excellent stability under continuous-flow operation.
Determinants of Inhibition of Transiently Expressed Voltage-gated Calcium Channels by ω-Conotoxins GVIA and MVIIA (2003)

The Conus magus peptide toxin omega-conotoxin MVIIA is considered an irreversible, specific blocker of N-type calcium channels, and is now in clinical trials as an intrathecal analgesic. Here, we have examined the action of MVIIA on mutant and wild type calcium channels transiently expressed in tsA-201 cells. Although we have shown previously that mutations in a putative external EF-hand motif in the domain IIIS5-H5 region alters block by both omega-conotoxin GVIA and MVIIA (Feng, Z. P., Hamid, J., Doering, C., Bosey, G. M., Snutch, T. P., and Zamponi, G. W. (2001) J. Biol. Chem. 276, 15728-15735), the introduction of five point mutations known to affect GVIA blocking (and located downstream of the EF-hand) affected MVIIA block to a smaller degree compared with GVIA. These data suggest that despite some overlap, MVIIA and GVIA block does not share identical channel structural determinants. At higher concentrations (approximately 3 microm), MVIIA reversibly blocked L-, P/Q-, and R-type, but not T-type channels, indicating that the overall architecture of the MVIIA site is conserved in all types of high voltage-activated calcium channels. A kinetic analysis of the MVIIA effects on the N-type channel showed that MVIIA blocked resting, open, and inactivated channels. Although the development of MVIIA block did not appear to be voltage-, nor frequency-dependent, the degree of recovery from block strongly depended on the potential applied during washout. Interestingly, the degree of washout was highly variable and appeared to weakly depend on the holding potential applied during toxin application. We propose a model in which N-type calcium channels can form both reversible and irreversible complexes with MVIIA.
Design Method for Aperture-Coupled Microstrip Patch Antennas on Textured Dielectric Substrates (2004)

Textured dielectric substrates made with multiple ceramics are now being introduced in antenna design. This paper presents a design technique for fabricating aperture-fed patch antennas with textured dielectrics. An example microstrip antenna is fabricated using this technique and measurements are compared to simulations. We show that by combining two ceramic materials a new composition is formed that has different properties from the original ceramics. These properties are measured and used in the fabrication of the antenna.
Supplementary Figure 3 from A Central Role for RAF→MEK→ERK Signaling in the Genesis of Pancreatic Ductal Adenocarcinoma (2023)

&lt;p&gt;PDF file - 194K, RAF and MEK co inhibition&lt;/p&gt;
Association between type 2 diabetes mellitus and disability: What is the contribution of diabetes risk factors and diabetes complications? (2018)

Diabetes is associated with a 67% increased risk of disability. Diabetes risk factors and comorbidities explain more of the association between diabetes and disability among South Asians than Africans. Obesity and history of CVD explained the largest percentage of the relationship between diabetes and disability, indicating that weight and CVD management may be helpful in controlling disability related to diabetes.
Oxidation resistance of carbides in chlorine-containing atmospheres (2002)

High temperature oxidation resistance of various carbides was investigated in air and in chlorine containing environments. Thermogravimetric oxidation tests were performed on fine powders of Cr 3 C 2 , TiC, TiCN, and WC in N 2 -20%0 2 and in N 2 -20%0 2 containing 2000 vppm HCl in the temperature range of 200-800C in order to clarify the effect of chlorine contairting species on the oxidation resistance of these carbides. The presence of chlorine increased the oxidation rate and also shifted the starting temperature of the oxidation process towards lower temperatures. Cr 3 C 2 was the most stable carbide in both atmospheres.
77‐4: A 2.78‐in 1058‐ppi Ultra‐High‐Resolution OLED Hybrid Display using Oxide Semiconductor/Oxide Conductor (OS/OC) Pixel (Transparent Pixel) achieving High Aperture Ratio (2017)

We prototyped a 2.78‐in 1058‐ppi OLED hybrid display. We employed an oxide semiconductor/oxide conductor (OS/OC) pixel (transparent pixel). In OS/OC pixels (transparent pixels), FETs, wirings, capacitors, and contact portions are transparent, achieving a high transmission area ratio of 66%.
Assessing multiple pollutant multiple source cancer risks from urban air toxics: Summary of approaches and insights from completed and ongoing urban air toxics-assessment studies (1989)

The primary purpose of this report is to assist State, local and other agency personnel by describing methods that have been used in assessing multiple source, multiple pollutant risks from air-toxics exposures in urban areas. The report does not constitute formal EPA guidance for conducting an urban risk assessment, nor does it recommend a single approach. Instead, it identifies techniques that others have elected to employ and offers insights that may assist the reader in selecting a particular set of techniques for use in a given locale. Major topics covered include (1) a summary of completed and ongoing urban air toxics assessment studies, (2) ambient monitoring assessment approaches, (3) emission inventory/dispersion modeling assessment approaches, (4) aspects of exposure and risk assessment, (5) control strategy evaluation, (6) data handling, and (7) evolving assessment technologies, including receptor modeling, personal monitoring, and bioassay sampling.
D2.3 Regulatory Framework and Business Models (2021)

The present document describes the regulatory framework and business models related to the INGENIOUS project. The regulatory framework has been approached from different perspectives. The document discusses the spectrum issues and frequency bands available in the regions where the use cases are taking place. It also describes the requirements and limitations at network level, security and privacy concerns, cryptocurrency aspects, as well as data protection issues. The deliverable also identifies and explores the current business models at strategic level that could be derived from the iNGENIOUS results. The document first provides a PESTEL analysis for providing context, and then tackles the technological enablers that may bring to the consortium new business models and opportunities. D2.3 also describes both regulatory and business aspects concerning each of the six use cases of iNGENIOUS.
Improved discrimination of VT from SVT in dual-chamber ICDs by combined analysis of dual-chamber intervals and ventricular electrogram morphology (2003)

The PR Logic dual-chamber defection algorithm discriminates VT/VF from SVT using hierarchical rules based on the pattern and timing of AA, VV, AV, and VA intervals. It cannot always discriminate rapidly-conducted atrial fibrillation(AF) from double tachycardia. Coexisting AF and VT/VF, or SVT with 1:1 antegrade conduction from VT with 1:1 retrograde conduction. This study evaluated a new tachyarrhythmia detection algorithm that supplements analysis of dual-chamber intervals with analysis of ventricular electrogram morphology based on the Wavelet Dynamic Discrimination Algorithm. Combined analysis of dual-chamber intervals and ventricular electrogram morphology reduced inappropriate detection of SVTs by 79% compared to dual chamber intervals alone without compromising sensitivity for detection of VT/VF.
On-line monitoring of gene expression (1999)

Gene expression in cultures of Escherichia coli has been determined in situ and on-line by the use of an electrochemical sensor. Intact bacteria were used to monitor the induction of the lacZ gene; the onset of stationary phase was also monitored, using a reporter gene fused to the RpoS-dependent promoter of the osmY gene. The technique described can in principle be used to determine the activity of any promoter, with a variety of reporter genes. This technology is non-intrusive, allows real-time monitoring of gene expression, and will be useful in the study of growth regulation and development.
Prostate carcinoma tumor size in rats decreases after administration of antagonists of luteinizing hormone-releasing hormone. (1982)

The effects of two potent antagonistic analogues of luteinizing hormone-releasing hormone (LH-RH) on the growth of two different models of rat prostate tumors have been investigated. Chronic administration of [NAc-p-F-DPhe1,p-Cl-DPhe2,DTrp3.6,D-Ala10]LH-RH (antagonist I) at 50 micrograms/day for 21 days significantly inhibited the growth of the chemically induced squamous cell carcinoma 11095 in Fisher 344 male rats. The weights of the pituitary, ventral prostate, and testes were not significantly altered. After 21 days of treatment with this analogue serum luteinizing hormone (lutropin), follicle-stimulating hormone, and testosterone levels were markedly decreased. When male Copenhagen F-1 rats bearing the Dunning 3327H prostate adenocarcinoma were injected with antagonist I at 50 micrograms/day for 6 weeks or with [NAc-p-Cl-DPhe1,2,DTrp3,DPhe6,DAla10]LH-RH (antagonist II) at 50 micrograms/day for 17 days, the percentage increase in tumor volume was decreased to half or less and the actual tumor volume was diminished 34-96% compared to controls. Tumor weight was decreased 30% and 89% after antagonist I and, respectively, compared to untreated controls. The tumor doubling time was 3- to 4-fold longer in rats receiving the inhibitory analogues than in the controls. Treatment with antagonist II decreased the weight of the whole prostate, but neither antagonist changed the weight of testes, anterior pituitary gland, or adrenals. Serum luteinizing hormone, follicle-stimulating hormone, and testosterone levels in Copenhagen F-1 rats bearing Dunning tumors were significantly decreased after treatment with the inhibitory analogues, but progesterone levels were increased. The inhibitory effects of these antagonistic analogues on rat prostate tumors suggest that these compound might be considered in the development of new types of therapy for prostate carcinoma and other endocrine-dependent neoplasias.
Folliculo-Stellate Cells in the Human Anterior Pituitary Express Cytokeratin (1993)

Recent studies have suggested that the anterior pituitary is derived not from Rathke's pouch, but instead from neuroectoderm. To address this controversy and the differentiation of the adenohypophysis, we evaluated folliculo-stellate cells from 7 human pituitaries using a panel of immunohistochemical stains. The panel included antibodies to a low molecular weight cytokeratin (CK-8), a broad spectrum of cytokeratins (AE 1/AE 3), S-100 protein, vimentin, and glial fibrillary acid protein. The most intense immunostaining was observed with the antibody to broad spectrum cytokeratins. Less intense immunohistochemical staining of folliculo-stellate cells was observed with the antibodies against glial fibrillary acidic protein and S-100 protein. Immunoblotting of anterior pituitary tissue obtained from 3 autopsied patients showed that cytokeratins identified in the anterior pituitary were of low molecular weight (40 to 56 Kd). These results indicate that folliculo-stellate cells show epithelial differentiation, and support the hypothesis that the anterior pituitary is derived from Rathke's pouch.
Cluster Based Deep Contextual Reinforcement Learning for top-k Recommendations (2020)

Rapid advancements in the E-commerce sector over the last few decades have led to an imminent need for personalised, efficient and dynamic recommendation systems. To sufficiently cater to this need, we propose a novel method for generating top-k recommendations by creating an ensemble of clustering with reinforcement learning. We have incorporated DB Scan clustering to tackle vast item space, hence in-creasing the efficiency multi-fold. Moreover, by using deep contextual reinforcement learning, our proposed work leverages the user features to its full potential. With partial updates and batch updates, the model learns user patterns continuously. The Duelling Bandit based exploration provides robust exploration as compared to the state-of-art strategies due to its adaptive nature. Detailed experiments conducted on a public dataset verify our claims about the efficiency of our technique as com-pared to existing techniques.
Biologic consequences of androgen receptor (AR) activity in MDV3100-resistant LNCaP cells and dependence on AR full length. (2014)

74 Background: While recent reports link androgen receptor (AR) variants (AR-Vs) to castration resistant prostate cancer (CRPC), the biological significance of AR-Vs in AR-regulated cell survival and proliferation, independent of AR full length (AR-FL), remains controversial. To define the functional role of AR-FL and AR-Vs in MDV3100-resistant (MDV-R), we designed antisense oligonucleotide (ASO) targeting exon 1 and exon 8 in AR to knockdown AR-FL alone or in combination with AR-Vs and examined these effects in MDV-R LNCaP-derived cells in vitro and in vivo. Methods: We generated by selection MDV-R LNCaP-derived sub-lines that uniformly expressed high levels of both AR-FL and AR-V7 compared to CRPC LNCaP xenografts. Cell growth rates, protein and gene expression were analyzed using crystal violet assay, western blotting and real-time PCR, respectively. Exon 1 and 8 AR-ASO were evaluated in MDV-R49F CRPC LNCaP xenografts. Results: AR-V7 was transiently transfected in MDV-R49F cells and differential knockdown of AR-V7 and/or AR-FL by exon 1 versus exon 8 AR-ASO was used to evaluate relative biologic contributions of AR-FL versus AR-V7 in MDV-R LNCaP AR-V7 overexpressing cells. Exon 1 and 8 AR-ASO treatment in these cells similarly decreased prostate-specific antigen (PSA) expression and induced apoptosis as measured by caspase-3 and PARP cleavage and cell growth inhibition. To further define the functional role of AR-Vs in MDV-R LNCaP cells, we used a CE3 siRNA that specifically silenced AR-V7, but not AR-FL in MDV-R LNCaP cells. AR-V7 knockdown did not decrease PSA levels, did not induce apoptosis, and did not inhibit cell growth. In MDV-R LNCaP cells, exon 1 and 8 ASO similarly suppressed cell growth and AR-regulated gene expression in vitro and in vivo. Conclusions: These results indicate that the AR remains an important driver of MDV3100 resistance and, the biologic consequences mainly driven by AR-FL in MDV-R LNCaP models.
Displaced humeral surgical neck fractures: classification and results of third-generation percutaneous intramedullary nailing (2018)

Antegrade insertion of a third-generation IMN through a percutaneous approach provides a high rate of fracture healing, excellent clinical outcome scores, and a low rate of complications. No morbidity related to the passage of the nail through the supraspinatus muscle and the cartilage was observed. The proposed A, B, and C classification allows choosing the optimal entry point for intramedullary nailing.
Detection and Quantification of Leveillula taurica Growth in Pepper Leaves (2013)

Leveillula taurica is an obligate fungal pathogen that causes powdery mildew disease on a broad range of plants, including important crops such as pepper, tomato, eggplant, onion, cotton, and so on. The early stage of this disease is difficult to diagnose and the disease can easily spread unobserved; for example, in pepper and tomato production fields and greenhouses. The objective of this study was to develop a detection and quantification method of L. taurica biomass in pepper leaves with special regard to the early stages of infection. We monitored the development of the disease to time the infection process on the leaf surface as well as inside the pepper leaves. The initial and final steps of the infection taking place on the leaf surface were consecutively observed using a dissecting microscope and a scanning electron microscope. The development of the intercellular mycelium in the mesophyll was followed by light and transmission electron microscopy. A pair of L. taurica-specific primers was designed based on the internal transcribed spacer sequence of L. taurica and used in real-time polymerase chain reaction (PCR) assay to quantify the fungal DNA during infection. The specificity of this assay was confirmed by testing the primer pair with DNA from host plants and also from another powdery mildew species, Oidium neolycopersici, infecting tomato. A standard curve was obtained for absolute quantification of L. taurica biomass. In addition, we tested a relative quantification method by using a plant gene as reference and the obtained results were compared with the visual disease index scoring. The real-time PCR assay for L. taurica provides a valuable tool for detection and quantification of this pathogen in breeding activities as well in plant-microbe interaction studies.
Investigations of Intermediate-Temperature Alkaline Methanol Fuel Cell Electrocatalysis Using a Pressurized Electrochemical Cell (2012)

Investigations of Intermediate-Temperature Alkaline Methanol Fuel Cell Electrocatalysis Using a Pressurized Electrochemical Cell
Functional principal component analysis (f-PCA) for the characterization of individual race walking performance strategies (2008)

Trichophyton benhamiae is a zoophilic dermatophyte transmitted to humans mostly from guinea pigs and occasionally other animals. It presents two distinct phenotypes: yellow and white. T. benhamiae was formerly known as Trichophyton species of Arthroderma benhamiae; it was considered part of the T. mentagrophytes species complex, and some authors have incorrectly described the yellow phenotype of T. benhamiae as T. mentagrophytes var. porcellae. Identification of T. benhamiae has been difficult, as it was described under more than three names, two phenotypes, and in several different possible host species. During the past 15 years, human infections due to this dermatophyte have been increasingly reported all over the world. In order to better understand the local epidemiology of T. benhamiae and to compare it to other European countries, we performed a 9-year retrospective study in the Strasbourg University Hospital. We studied 41 dermatophytes (38 isolated from humans and 3 from guinea pigs) identified as T. mentagrophytes var. porcellae or A. benhamiae from January 2008 to December 2016 and verified their identification by ITS (Internal Transcribed Spacer) sequencing. ITS sequencing was performed in 35 of the 41 strains, and they were identified as T. benhamiae (33), T. bullosum (1), and T. eriotrephon (1). The other six remaining strains were identified according to morphology as T. mentagrophytes var. porcellae, name incorrectly used since 2010 for the yellow phenotype of T. benhamiae. ITS sequencing is recommended for accurate identification of this dermatophyte and the culture phenotype (yellow or white) should be specified.
ImageFlow: Streaming Image Search (2010)

Intrinsically disordered proteins (IDPs) as well as intrinsically disordered regions (IDRs) of complex protein machineries have recently been recognized as key players in many cellular functions. NMR represents a unique tool to access atomic resolution structural and dynamic information on highly flexible IDPs/IDRs. Improvements in instrumental sensitivity made heteronuclear direct detection possible for biomolecular NMR applications. The CON experiment has become one of the most useful NMR experiments to get a snapshot of an IDP/IDR in conditions approaching physiological ones. The availability of NMR spectrometers equipped with multiple receivers now enables the acquisition of several experiments simultaneously instead of one after the other. Here, we propose several variants of the CON experiment in which, during the recovery delay, a second two-dimensional experiment is acquired, either based on <sup>1</sup>H detection (CON//HN) or on <sup>15</sup>N detection (CON//btNH, CON//(H)CAN). The possibility to collect simultaneous snapshots of an IDP/IDR through different two-dimensional spectra provides a novel tool to follow chemical reactions, such as the occurrence of posttranslational modifications, as well as to study samples of limited lifetime such as cell lysates or whole cells.
Polypyrrole–Peptide Microarray for Biomolecular Interaction Analysis by SPR Imaging (2009)

Nowadays, high-throughput analysis of biological events is a great challenge which could take benefit of the recent development of microarray devices. The great potential of such technology is related to the availability of a chip bearing a large set of probes, stable and easy to obtain, and suitable for ligand-binding detection. Here, we describe a new method based on polypyrrole chemistry, allowing the covalent immobilization of peptides in a microarray format and on a gold surface compatible with the use of surface plasmon resonance. This technique is then illustrated by the detection and characterization of antibodies induced by hepatitis C virus and present in patients' serums.
Trace Gas Analyzers Based on Tunable Diode Laser Absorption Spectroscopy (TDLAS) for Energy Production, Transmission, and Storage (2009)

This paper describes energy-related uses of TDLAS. Applications include improving the efficiency and quality of natural gas transmission, monitoring and verifying the fate of CO2 at geological sequestration sites, and monitoring contaminants in hydrogen fuels.
trans-Hydroalumination-Alkylation of Propargylic Alcohols (2006)

This is a simple method for the trans-hydroalumination-alkylation of propargylic alcohols, giving stereochemically defined trisubstituted allylic alcohols. This method complements the known procedure for the cis-hydroalumination-alkylation of allylic alcohols with DIBAL-H. The method uses inexpensive and relatively safe Vitride (Red-Al) as the hydroalumination agent. The method is not sensitive to the steric hindrance in the allylic alcohol. Only reactive electrophiles like allylic, benzylic and propargylic halides afford good yields of products.