gebhart/patrepeval huggingface dataset:
P1‐355: Mechanistic studies of antibody mediated clearance of tau aggregates using an ex vivo brain slice model (2010)

Our group has shown in two models with tangle pathology that immunization with an AD specific phospho-tau epitope slows the progression of tangle-associated phenotype, reduces tau aggregates and prevents cognitive impairments (Asuni A. et al. J. Neurosci., 2007, Sigurdsson E.M. et al., ICAD Chicago, 2008). Additionally, we demonstrated that immunization against a phospho-tau epitope located within the microtubule binding region could also clear tau aggregates and reduce functional deficits in a mouse model of tauopathy (Krishnamurthy P.K. et al., ICAD Vienna, 2009). A natural follow up of these studies is to understand the cellular mechanisms involved in this phenomenon. Therefore, the uptake and localization of FITC labeled anti-tau antibodies was studied in an organotypic brain slice model derived from adult JNPL3 (P301L) mice with established tau pathology. Brain slices were prepared, allowed to stabilize and incubated with FITC-IgG for two hours at 35 °C with intermittent oxygenation. The IgG was purified from an immunized P301L mouse with a high antibody titre against our phos-tau immunogen (Tau 379-408[pSer396,404]). Slices (400 μm) were fixed in PLP and 40 μm sections from their center were stained with the tau antibodies CP13 (pSer202 epitope) and MC1 (conformational epitope) and a Texas Red conjugated secondary. Sections were also stained with antibodies against LAMP2 and rab5, markers of lysosomes and early endosomes respectively. All sections were counterstained with the nuclear marker Hoescht 33342 and visualized by confocal microscopy. Extensive but partial co-localization was observed between FITC-IgG and the tau antibodies CP13 and MC1. Furthermore, we determined that the FITC-IgG was completely associated with cellular markers of lysosomes, LAMP2, and early endosomes, rab5, with perinuclear vesicles as the main areas of co-staining. In wild-type mice, limited non-specific FITC-IgG binding was observed. Biochemical studies are underway to examine antibody compartmentalization. We are assessing as well the ubiquitin-proteosome system that is probably not involved as it is likely saturated under these conditions. These findings indicate that the endosomal-lysosomal pathway is involved in antibody-mediated clearance of tau aggregates. Patent pending on tau immunotherapy.
Signal processor implementation of decimation filters in flexible receivers (2003)

Configurable or programmable hardware is used much more than some years ago in wireless receivers. In this area, the need to develop small sized mobile terminals operating flexibly in different systems is receiving increasing attention. In this paper, we describe a programmable solution for sampling rate adaptation in a flexible radio focusing on the GSM mode. We present the hardware needed for the overall architecture and the implementation aspects of the solution based on the signal processor families TMS320C62x and TMS320C55x.
In Vivo Two‐photon Fluorescence Microscopy Reveals Disturbed Cerebral Capillary Blood Flow and Increased Susceptibility to Ischemic Insults in Diabetic Mice (2014)

Diabetic mice are more susceptible to disturbances of cerebral capillary blood flow which may predispose them to neurovascular defects following ischemia.
A Novel Multi-AP Diversity for Highly Reliable Transmissions in Wireless LANs (2021)

With the development of the IEEE 802.11 standard for wireless LANs, there has been an enormous increase in the usage of wireless LANs in factories, plants, and other industrial environments. In industrial applications, wireless LAN systems require high reliability for stable real-time communications. In this paper, we propose a multi-access-point (AP) diversity method that contributes to the realization of robust data transmissions toward realization of ultra-reliable low-latency communications (URLLC) in wireless LANs. The proposed method can obtain a diversity effect of multipaths with independent transmission errors and collisions without modification of the IEEE 802.11 standard or increasing overhead of communication resources. We evaluate the effects of the proposed method by numerical analysis, develop a prototype to demonstrate its feasibility, and perform experiments using the prototype in a factory wireless environment. These numerical evaluations and experiments show that the proposed method increases reliability and decreases transmission delay.
Probabilistic Indexing and Categorisation Tool, Intermediate Prototype (1999)

WP4 deals with automatic categorisation of web documents that is based on a description oriented approach to document indexing. This deliverable describes further progress with respect to the work done in Deliverable 4.1 as well as an Intermediate Prototype which implements parts of the architecture given in Deliverable 4.1.
Luminescence and intramolecular energy-transfer processes in isomeric cyano-bridged rhenium(I)-rhenium(I) and rhenium(I)-ruthenium(II)-rhenium(I) polypyridyl complexes (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLuminescence and intramolecular energy-transfer processes in isomeric cyano-bridged rhenium(I)-rhenium(I) and rhenium(I)-ruthenium(II)-rhenium(I) polypyridyl complexesK. Kalyanasundaram, M. Graetzel, and M. K. NazeeruddinCite this: Inorg. Chem. 1992, 31, 25, 5243–5253Publication Date (Print):December 1, 1992Publication History Published online1 May 2002Published inissue 1 December 1992https://pubs.acs.org/doi/10.1021/ic00051a016https://doi.org/10.1021/ic00051a016research-articleACS PublicationsRequest reuse permissionsArticle Views447Altmetric-Citations55LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Vapor Transport of Gallium in the Ga-PCl3-H2 System (1971)

A theoretical consideration based on thermodynamics has been developed and the result is compared with experimental results obtained for the transport of gallium. The transport rate of gallium is discussed as a function of the reaction temperature, the hydrogen flow rate and the mole ratio of Cl/H. A good agreement is found between the theory and experimental results. It is also shown that the experimental data of Luther and Roccasecca are predicted by the theory and that the theoretical results give an upper limit of the transport rate of the gallium.
An online guided e‐journal exercise in pre‐clerkship years: Oxidative phosphorylation in brown adipose tissue (2014)

The rationale for this mandatory, guided online e-journal exercise is to foster the ability of students to independently read medical and scientific literature in a critical manner and to integrate journal reading with their basic science knowledge. After a lecture on oxidative phosphorylation, students were assigned to read an article on brown adipose tissue published in New England Journal of Medicine and were guided to analyze the article by answering online questions. After two iterations, student surveys about the project, its key pedagogical features, and ways to improve it suggest that the students perceived these exercises as active learning, which is clinically relevant and built on their course material. Furthermore, students agreed that the e-journal project was useful for learning how to read an article, for reviewing the material learned in class, and for promoting evidence-based medicine. This online e-journal exercise models some aspects students will experience as future physicians, where it is essential to keep up with literature and extract relevant information on a tight physician's schedule. This study demonstrated the usefulness of guided e-journal exercises as a simple effective active teaching tool for preclinical medical students, which can also be used for prehealth undergraduate programs.
Aggregation kinetics and shear rheology of aqueous silica suspensions (2012)

The kinetics of aggregation of silica nanoparticle solutions as a function of NaCl and silica concentrations is studied experimentally and theoretically. Silica nanoparticles form fractal aggregates due to the collapse of the electrical double layer at high salt concentrations and resulting reduction in stabilizing repulsive force. We propose a convenient model to describe the aggregation of silica nanoparticles and the growth of their aggregate size that depends on particle size and concentration and salt concentration. The model agrees well with experimental data. The aggregation of silica nanoparticles also affects the rheology of the suspension. We propose an equilibrium approach for sediment volume fraction to determine the maximum effective packing fraction. The results for the relative viscosity of silica aggregates agree well with the proposed viscosity model, which also collapses onto a single master curve.
Sustained delivery of recombinant human bone morphogenetic protein‐2 from perlecan domain I ‐ functionalized electrospun poly (ε‐caprolactone) scaffolds for bone regeneration (2016)

We conclude that this platform provides a sophisticated and efficient approach to deliver bioactive rhBMP-2 for bone tissue regeneration applications.
ACP-OPLL photonic integrated circuit for high dynamic range RF/photonic links (2011)

Fiber-optic links are attractive for transmitting microwave/millimeter wave signals for applications such as radar, imaging and astronomy. However, current fiber-optic links that employs intensity modulation and direct detection suffer from limited spurious free dynamic range (SFDR). For solution, a new coherent fiber-optic link using linear phase modulation/demodulation has been proposed. The new link should be able to achieve an SFDR two orders of magnitude higher. The key for this link is an Optical Phase Locked Loop (OPLL) linear phase demodulator. In this paper we describe the design and preliminary measurements for the first generation ACP-OPLL photonic integrated circuits.
Stretchable electrically conductive and high gas barrier nanocomposites (2018)

By alternately depositing trilayers of polyethylene oxide, double-walled carbon nanotubes stabilized in polyacrylic acid, and graphene oxide <italic>via</italic> layer-by-layer assembly, elastomeric conductive multilayers with a high gas barrier were prepared.
Abstract 2601: Gingerol aspirinate derivatives as novel chemopreventive agents for colon cancer (2016)

Abstract Regular aspirin use has been convincingly shown to reduce the risk of colorectal cancer. However, long-term use of aspirin leads to gastrotoxicity. Herein, we designed and synthesized a novel class of gingerol- and shogaol-based aspirin prodrugs to simultaneously release aspirin and gingerol or shogaol to attenuate the side effects caused by aspirin. Prodrug GAS exerted enhanced anticancer activities, which are better than a physical mixture of aspirin and 6-gingerol as well as each individual. In addition, GAS eliminated the gastric toxicity induced by aspirin in mice. Metabolism of GAS in mice showed that the majority of GAS is decomposed to release 6-gingerol and aspirin or salicylic acid either in the intestine or after absorption. Mechanistic studies demonstrate GAS could dose-dependently inhibit COX pathway. These findings highlighted the improved anticancer properties of gingerol-based aspirin prodrugs. GAS may represent novel and safe alternatives of aspirin for the purpose of daily use in the future. Citation Format: Yingdong Zhu, Fang Wang, Yantao Zhao, Pei Wang, Shengmin Sang. Gingerol aspirinate derivatives as novel chemopreventive agents for colon cancer. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 2601.
Supplementary Figure 2 from Activation of the <i>NOTCH</i> Pathway in Head and Neck Cancer (2023)

&lt;p&gt;PDF file - 169K, NOTCH pathway activation in HNSCC. A, Immunohistochemical analysis of NOTCH1, HES1 and HEY1 protein expression in HNSCC. Tissue microarray arrays with quadruplicates of samples from each of 56 HNSCC tumor and 11 non-tumor patients were stained with anti-NOTCH1, anti-HES1 or anti-HEY1 antibody, as described in methods. Top, the heat-map of immunohistochemical analysis for tissue microarray slides. Rows, genes; column, each individual sample. The average staining for four tissues was categorized to Strong (brown), Moderate (grey) or Weak/Negative (blue) staining. Samples are ranked by the pathology ID numbers in tumor (left) and normal (right) groups. Bottom: quantification of immunohistochemical data. The number and percentage of tumor (Tu) and normal (No) patient in strong, moderate and weak/negative category was quantified. P-values were calculated by comparing numeric scores in tumor vs normal sample by independent t test. The samples 57964, 58602, 22146, 5931, 26514 represent tumor samples X6, X7, X11, X28 and X34 from the original discovery cohort of 44 HNSCC tumors. B, Representative immunohistochemical results. The representative slides of positive or moderate staining are shown for tumors and compared to normal tissue. Bar, 100 um equivalent. Tumor (T) and normal (N) tissues on each sample are labeled. ID 22146 represented X11 samples, which were used both in array and in immunohistochemical analysis, where protein and RNA expression strongly correlate.&lt;/p&gt;
Neuroimaging of amyloid pathology in a non‐human primate model of sporadic CAA (2020)

Abstract Background There has been a growing interest in developing MRI technologies to visualize Aβ plaques in animals, using approaches that may eventually be applied to humans. A non‐human primate model, squirrel monkey (SQM), was utilized in the present study due to their unique property of naturally developing age‐dependent cerebral amyloid angiopathy (CAA) identical to that seen in humans. It has been demonstrated that the transverse relaxation rate (R2*) has a linear correlation with iron content, a component of amyloid deposits and microhemorrhages. Here we proposed to evaluate whether the region of interest (ROI)‐based quantitative R2* assessment is able to detect age related differences in SQM neuropathology. An additional focus was to validate the ability of our novel MRI methodology using bi‐functional nanoparticles (USPIO‐PEG‐Aβ) to detect amyloid deposits. Method Multi‐gradient echo MR images were acquired in three different SQM age cohorts (ages 5, 16, 21) at various stages of Aβ pathology development. The R2* map of pre‐ and post‐Aβ nanoprobe injection MRI scans were generated (Firevoxel parametric map tool) from each subject to enable (ROI)‐based quantitative R2* analyses. 6E10 immunohistochemistry was used to confirm the presence of CAA and parenchymal plaques to correlate with the R2*. Result MGE imaging was effective in differentiating between the MRI brains of a 5 year old SQM with no Aβ deposits and aged SQMs with established Aβ pathology. Absolute quantitation of R2* values, defined by ROI circumventing the cortex, revealed significant differences between the young and old subjects , with the highest R2* counts detected in the 21 year old monkey. Further increase in R2* values was observed 36 hrs post‐contrast injection scans in specific brain regions in our oldest cohort, indicative of plaque labeling with our contrast agent. No toxicity was observed during the scans, demonstrating the safety of our Aβ nanoprobe. The degree of correspondence between amyloid deposits detected by MRI and 6E10 immunohistochemistry was confirmed via matched histological sections. Conclusion The present findings validate the utility of our MRI methodologies to monitor age dependent accumulation of Aβ deposits, opening the possibility of longitudinally evaluating novel therapeutic interventions targeting amyloid pathology.
Design and Implementation of Three-Phase Two-Stage Grid-Connected Module Integrated Converter (2014)

Module integrated converters (MICs) in single phase have witnessed recent market success due to unique features such as improved energy harvest, improved system efficiency, lower installation costs, plug-and-play operation, and enhanced flexibility and modularity. The MIC sector has grown from a niche market to mainstream, especially in the United States. Assuming further expansion of the MIC market, this paper presents the microinverter concept incorporated in large size photovoltaic (PV) installations such as megawatts (MW)-class solar farms where a three-phase ac connection is employed. A high-efficiency three-phase MIC with two-stage zero voltage switching (ZVS) operation for the grid-tied PV system is proposed which will reduce cost per watt, improve reliability, and increase scalability of MW-class solar farms through the development of new solar farm system architectures. The first stage consists of a high-efficiency full-bridge LLC resonant dc-dc converter which interfaces to the PV panel and produces a dc-link voltage. A center points iteration algorithm developed specifically for LLC resonant topologies is used to track the maximum power point of the PV panel. The second stage is comprised of a three-phase dc-ac inverter circuit which employs a simple soft-switching scheme without adding auxiliary components. The modeling and control strategy of this three-phase dc-ac inverter is described. Because the dc-link capacitor plays such an important role for dual-stage MIC, the capacitance calculation is given under type D voltage dip conditions. A 400-W prototype was built and tested. The overall peak efficiency of the prototype was measured and found to be 96% with 98.2% in the first stage and 98.3% in the second stage.
Rapid and Reliable Startup of Biological Wastewater Treatment Systems in Space (2019)

Bill Cumbie, Pancopia, Inc., USA
Direct Photopatterning of Electrochromic Polymers (2013)

Abstract Propylenedioxythiophene (ProDOT) polymers are synthesized using an oxidative polymerization route that results in methacrylate substituted poly(ProDOTs) having a M n of 10–20 kDa wherein the methacrylate functionality constitutes from 6 to 60% of the total monomer units. Solutions of these polymers show excellent film forming abilities, with thin films prepared using both spray‐casting and spin‐coating. These polymers are demonstrated to crosslink upon UV irradiation at 350 nm, in the presence of an appropriate photoinitiator, to render the films insoluble to common organic solvents. Electrochemical, spectroelectrochemical, and colorimetric analyses of the crosslinked polymer films are performed to establish that they retain the same electrochromic qualities as the parent polymers with no detriment to the observed properties. To demonstrate applicability for multi‐film processing and patterning, photolithographic patterning is shown, as is desired for fully solution processed and patterned devices.
Molecular-beam studies of the precursor-mediated dissociative chemisorption of N2 on W(100) (1989)

Molecular-beam techniques have been employed to probe various aspects of the dynamics of the dissociative chemisorption of N2 on W(100). The dissociative chemisorption probability has been measured over a wide range of incidence energies Ei, angles θi, and surface temperatures Ts. This probability is found to fall rapidly with increasing Ei in the range 26–450 meV, where it is also found to fall rapidly with Ts, and to be relatively insensitive to surface coverage at low Ts. In addition, resu1ts are found to be surprisingly insensitive to the incidence angle over the range 0°–70°. This behavior has been interpreted in terms of a precursor model, noting that the trapping process must scale quite closely with the total incidence energy. Support for these conclusions comes from careful measurements of the angular and velocity distributions of scattered molecules which has also revealed that increasing Ts primarily serves to reduce the fraction of precursor molecules that go on to dissociate, by biasing the kinetics in favor of desorption. In contrast, the trapping probability into the precursor state is found to be relatively insensitive to Ts, accounting for ≲20% of the observed effects. Measurements have also been made of the trapping probability of N2 on the W(100) surface covered with N and N2 species. We find that while trapping is insensitive to coverage for Ei &amp;lt;0.1 eV, it varies appreciably with coverage at higher energies; being highest on the surface saturated with both atoms and molecules followed by the clean surface, with the lowest trapping probability on the surface saturated with atoms.
Linking the US Transplant Registry to Administrative Claims Data: Expanding the Potential of Transplant Research (2007)

This comparison of payer data with information reported by transplant centers to the OPTN provides important insight into the value of both data sources. Using administrative claims to augment the registry data with utilization and cost information will be useful for evaluation of both economic and clinical endpoints in solid organ transplantation.
Engineering genetically-encoded synthetic biomarkers for breath-based cancer detection (2021)

Abstract Breath analysis holds great promise for rapid, noninvasive early cancer detection; however, clinical implementation is impeded by limited signal from nascent tumors and high background expression by non-malignant tissues. To address this issue, we developed a novel breath-based reporter system for early cancer detection using D-limonene, a volatile organic compound (VOC) from citrus fruit that is not produced in humans, in order to minimize background signal and maximize sensitivity and specificity for cancer detection. We metabolically engineered HeLa human cervical cancer cells to express limonene at levels detectable by mass spectrometry by introducing a single plant gene encoding limonene synthase. To improve limonene production and detection sensitivity twofold, we genetically co-expressed a modified form of a key enzyme in the cholesterol biosynthesis pathway. In a HeLa xenograft tumor mouse model, limonene is a sensitive and specific volatile reporter of tumor presence and growth, permitting detection of tumors as small as 5 mm. Moreover, tumor detection in mice improves proportionally with breath sampling time. By continuously collecting VOCs for 10 hours, we improve sensitivity for cancer detection 100-fold over static headspace sampling methods. Whole-body physiologically-based pharmacokinetic (PBPK) modeling and simulation of tumor-derived limonene predicts detection of tumors as small as 7 mm in humans, equivalent to the detection limit of clinical imaging modalities, such as PET, yet far more economical. Significance Statement We developed a breath-based reporter system using the plant terpene, D-limonene – a volatile secondary metabolite that gives citrus fruit its characteristic scent but is not produced in human tissues – as a biomarker for early cancer detection. Results from this study could pave the way for in vivo gene delivery and tumor-specific expression of exogenous volatile cancer reporters with broad applicability to the early diagnosis of a wide variety of cancers.