gebhart/patrepeval huggingface dataset:
Both Feline Coronavirus Serotypes 1 and 2 Infected Domestic Cats Develop Cross-Reactive Antibodies to SARS-CoV-2 Receptor Binding Domain: Its Implication to Pan-CoV Vaccine Development (2023)

The current study was initiated when our specific-pathogen-free laboratory toms developed unexpectedly high levels of cross-reactive antibodies to human SARS-CoV-2 (SCoV2) receptor binding domain (RBD) upon mating with feline coronavirus (FCoV)-positive queens. Multi-sequence alignment analyses of SCoV2 Wuhan RBD and four strains each from FCoV serotypes 1 and 2 (FCoV1 and FCoV2) demonstrated an amino acid sequence identity of 11.5% and a similarity of 31.8% with FCoV1 RBD (12.2% identity and 36.5% similarity for FCoV2 RBD). The sera from toms and queens cross-reacted with SCoV2 RBD and reacted with FCoV1 RBD and FCoV2 spike-2, nucleocapsid, and membrane proteins, but not with FCoV2 RBD. Thus, the queens and toms were infected with FCoV1. Additionally, the plasma from six FCoV2-inoculated cats reacted with FCoV2 and SCoV2 RBDs, but not with FCoV1 RBD. Hence, the sera from both FCoV1-infected cats and FCoV2-infected cats developed cross-reactive antibodies to SCoV2 RBD. Furthermore, eight group-housed laboratory cats had a range of serum cross-reactivity to SCoV2 RBD even 15 months later. Such cross-reactivity was also observed in FCoV1-positive group-housed pet cats. The SCoV2 RBD at a high non-toxic dose and FCoV2 RBD at a 60-400-fold lower dose blocked the in vitro FCoV2 infection, demonstrating their close structural conformations essential as vaccine immunogens. Remarkably, such cross-reactivity was also detected by the peripheral blood mononuclear cells of FCoV1-infected cats. The broad cross-reactivity between human and feline RBDs provides essential insights into developing a pan-CoV vaccine.
Acid catalysis in the formation of dioxopiperazines from peptides containing tetrahydroisoquinoline‐3‐carboxylic acid at position 2 (1995)

The kinetics of the spontaneous formation of 2,5-dioxopiperazines from peptides containing the Tic (1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid) residue in the 2-position of the sequence has been studied in DMSO and water solution. The reaction is first order in Tic-peptide and subject to general-acid catalysis. Moreover, only the fraction of peptide having the amino terminal group in the deprotonated state reacts with appreciable rate. In pure organic solvent, and in aqueous solution with low buffer concentration, the degradation reaction of Tic-peptides is very low; at 20 degrees C for the peptide H-Tyr-Tic-Phe-Phe-NH2, in DMSO and in neutral water in the absence of buffer, the half-lives (t1/2) are 3 x 10(4) and 1.2 x 10(4) h, respectively. The addition of carboxylic acids or buffers to the reaction solutions markedly increases the reaction rate; in 0.01 m HAc in DMSO and in 0.1 M phosphate buffer in water, pH 7.1, t1/2 values for the tetrapeptide are 61 and 121 h, respectively.
13-µm optically-pumped semiconductor disk laser by wafer fusion (2009)

We report a wafer-fused high power optically-pumped semiconductor disk laser operating at 1.3 microm. An InP-based active medium was fused with a GaAs/AlGaAs distributed Bragg reflector, resulting in an integrated monolithic gain mirror. Over 2.7 W of output power, obtained at temperature of 15 degrees C, represents the best achievement reported to date for this type of lasers. The results reveal an essential advantage of the wafer fusing technique over both monolithically grown AlGaInAs/GaInAsP- and GaInNAs-based structures.
Complexes Derived from Strong Field Ligands. XV. The Formation of Trinuclear Complexes by Coördination of Tris(β-mercaptoethylamine)-cobalt(III) to Metal Ions (1962)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTComplexes Derived from Strong Field Ligands. XV. The Formation of Trinuclear Complexes by Coördination of Tris(β-mercaptoethylamine)-cobalt(III) to Metal IonsDaryle H. Busch and Donald C. JichaCite this: Inorg. Chem. 1962, 1, 4, 884–887Publication Date (Print):November 1, 1962Publication History Published online1 May 2002Published inissue 1 November 1962https://pubs.acs.org/doi/10.1021/ic50004a036https://doi.org/10.1021/ic50004a036research-articleACS PublicationsRequest reuse permissionsArticle Views247Altmetric-Citations90LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
In VitroProliferation of Porcine Pancreatic Islet Cells forβ-Cell Therapy Applications (2016)

<i>β</i>-Cell replacement through transplantation is the only curative treatment to establish a long-term stable euglycemia in diabetic patients. Owing to the shortage of donor tissue, attempts are being made to develop alternative sources of insulin-secreting cells. Stem cells differentiation and reprograming as well as isolating pancreatic progenitors from different sources are some examples; however, no approach has yet yielded a clinically relevant solution. Dissociated islet cells that are cultured in cell numbers by <i>in vitro</i> proliferation provide a promising platform for redifferentiation towards <i>β</i>-cells phenotype. In this study, we cultured islet-derived cells <i>in vitro</i> and examined the expression of <i>β</i>-cell genes during the proliferation. Islets were isolated from porcine pancreases and enzymatically digested to dissociate the component cells. The cells proliferated well in tissue culture plates and were subcultured for no more than 5 passages. Only 10% of insulin expression, as measured by PCR, was preserved in each passage. High glucose media enhanced insulin expression by about 4-18 fold, suggesting a glucose-dependent effect in the proliferated islet-derived cells. The islet-derived cells also expressed other pancreatic genes such as Pdx1, NeuroD, glucagon, and somatostatin. Taken together, these results indicate that pancreatic islet-derived cells, proliferated <i>in vitro</i>, retained the expression capacity for key pancreatic genes, thus suggesting that the cells may be redifferentiated into insulin-secreting <i>β</i>-like cells.
Concept, design and pre-clinical studies for remote control percutaneous coronary interventions. (2005)

A system that enables remote control percutaneous coronary procedures was developed and tested in vitro and in vivo models. Preclinical studies have demonstrated the feasibility of the concept of remote control PCI and have provided the basis for the pilot clinical study of remote control, stent-assisted PCI.
Novel Hypoxia-Inducible Factor 1α (HIF-1α) Inhibitors for Angiogenesis-Related Ocular Diseases: Discovery of a Novel Scaffold via Ring-Truncation Strategy (2018)

Ocular diseases featuring pathologic neovascularization are the leading cause of blindness, and anti-VEGF agents have been conventionally used to treat these diseases. Recently, regulating factors upstream of VEGF, such as HIF-1α, have emerged as a desirable therapeutic approach because the use of anti-VEGF agents is currently being reconsidered due to the VEGF action as a trophic factor. Here, we report a novel scaffold discovered through the complete structure-activity relationship of ring-truncated deguelin analogs in HIF-1α inhibition. Interestingly, analog 6i possessing a 2-fluorobenzene moiety instead of a dimethoxybenzene moiety exhibited excellent HIF-1α inhibitory activity, with an IC<sub>50</sub> value of 100 nM. In particular, the further ring-truncated analog 34f, which showed enhanced HIF-1α inhibitory activity compared to analog 2 previously reported by us, inhibited in vitro angiogenesis and effectively suppressed hypoxia-mediated retinal neovascularization. Importantly, the heteroatom-substituted benzene ring as a key structural feature of analog 34f was identified as a novel scaffold for HIF-1α inhibitors that can be used in lieu of a chromene ring.
Wind Farm Grid Integration Using VSC Based HVDC Transmission - An Overview (2008)

The paper gives an overview of HVAC and HVDC connection of wind farm to the grid, with an emphasis on voltage source converter (VSC)-based HVDC for large wind farms requiring long distance cable connection. Flexible control capabilities of a VSC-based HVDC system enables smooth integration of wind farm into the power grid network while meeting the grid code requirements (GCR). Operation of a wind farm with VSC-based HVDC connection is described.
Epitaxial rare earth oxide and nitride buffers for GaN growth on Si (2014)

Abstract The erbium oxide and nitride layers were grown as a buffer for GaN on Si(111). Engineering of the nucleation layer on the oxide plays a crucial role in the quality of the GaN layer. The intermediate erbium nitride layer with lattice constant between that of GaN and Si additionally reduces the semiconductor layer lattice mismatch to the substrate and prevents direct bonding of gallium atoms with the oxygen of the rare‐earth oxide. The crystal structure and surface morphology of epitaxial single crystal gallium nitride layer grown on the buffer improves with the semiconductor layer thickness. (© 2014 WILEY‐VCH Verlag GmbH &amp; Co. KGaA, Weinheim)
Purification and characterization of the trefoil peptide human spasmolytic polypeptide (hSP) produced in yeast (1993)

Recombinant human spasmolytic polypeptide (r-hSP) has been produced in relatively large amounts in Saccharomyces cerevisiae. The two intronless trefoil domains of the hSP-DNA were cloned separately by PCR from human genomic DNA, and the remaining parts of the gene synthesized. Recombinant plasmids were constructed to encode a fusion protein consisting of a hybrid leader sequence and the hSP sequence. The leader sequence serves to direct the fusion protein into the secretory pathway of the cell and to expose it to the Kex 2 processing enzyme system. The secreted r-hSP was found in a glycosylated and an non-glycosylated form. The two forms of r-hSP were purified from the yeast fermentation broth by a combination of ion-exchange chromatography and preparative HPLC. The overall yield from 8 litres of fermentation broth was 160 mg r-hSP and 219 mg glycosylated r-hSP corresponding to 50% and 34%, respectively. The structure of the r-hSP and the glycosylated r-hSP was determined by amino acid analysis and carbohydrate composition analysis as well as by peptide mapping, amino acid sequencing and mass spectrometric analysis.
Application of Liquid-Phase Direct Fluorination: Novel Synthetic Methods for a Polyfluorinated Coating Material and a Monomer of a Perfluorinated Polymer Electrolyte Membrane (2012)

A new polyfluorinated anti-staining coating material CF3O(CF2CF2O)xCF2-CONHCH2CH2CH2Si(OCH3)3 has been developed by utilizing the PERFECT method, which employs a liquid-phase direct fluorination reaction with elemental fluorine as a key step. Direct fluorination of a partially-fluorinated ester, which was prepared from a non-fluorinated poly(ethylene glycol) and a perfluorinated acyl fluoride, followed by methanolysis, gave the perfluorinated corresponding compound, which was led to the coating material for surface treating agents, and the methyl ester of the starting perfluorinated acyl fluoride. Application to the synthesis of a new perfluorinated bifunctional sulfonate monomer CF2=CFOCF2CF2CF2OCF(CF2SO2F)2 for polymer electrolyte membranes (PEMs) of fuel cells was also developed.
Supplementary Methods from <i>De novo</i> Lipogenesis Protects Cancer Cells from Free Radicals and Chemotherapeutics by Promoting Membrane Lipid Saturation (2023)

Supplementary Methods from &lt;i&gt;De novo&lt;/i&gt; Lipogenesis Protects Cancer Cells from Free Radicals and Chemotherapeutics by Promoting Membrane Lipid Saturation
Autonomous transaction execution with epsilon serializability (2003)

The authors study the feasibility of autonomous transaction execution in systems with asynchronous transaction processing based on epsilon serializability (ESR). The abstract correctness criteria defined by ESR are implemented by techniques such as asynchronous divergence control and asynchronous consistency restoration. Concrete application examples in a distributed environment, such as banking, illustrate the advantages of using ESR to support execution autonomy. The ability for asynchronous transaction processing also supports efficient concurrent transaction and query processing.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
The int-1 proto-oncogene. (1986)

The int-1 proto-oncogene is the first cellular gene discovered and implicated in tumorigenesis solely on the basis of repeated insertional mutations that activate transcription of the gene. The gene is silent in most tissues but expressed in the embryonic central nervous system, in the late (post-meiotic) stages of spermatogenesis, and in a high proportion of mouse mammary tumor virus-induced carcinomas, when a provirus is inserted upstream or downstream of the coding domain. The functional significance of int-1 in the oncogenic process is supported by the demonstration that murine leukemia virus-based vectors carrying the gene can alter the morphology and growth properties of an established line of mammary epithelial cells. The predicted primary protein product of the int-1 gene is 370 amino acids in length and cysteine-rich; immunoprecipitation with anti-peptide antibodies reveals multiple species of int-1 protein, due to asparagine-linked glycosylations and probable cleavage of a signal peptide. However, the active product of the gene and its biochemical behavior during normal development and mammary tumorigenesis are not known.
Pomegranate Fruit Extract Modulates UV‐B–mediated Phosphorylation of Mitogen‐activated Protein Kinases and Activation of Nuclear Factor Kappa B in Normal Human Epidermal Keratinocytes¶ (2005)

ABSTRACT Excessive exposure of solar ultraviolet (UV) radiation, particularly its UV‐B component, to humans causes many adverse effects that include erythema, hyperplasia, hyperpigmentation, immunosuppression, photoaging and skin cancer. In recent years, there is increasing use of botanical agents in skin care products. Pomegranate derived from the tree Punica granatum contains anthocyanins (such as delphinidin, cyanidin and pelargonidin) and hydrolyzable tannins (such as punicalin, pedunculagin, punicalagin, gallagic and ellagic acid esters of glucose) and possesses strong antioxidant and anti‐inflammatory properties. Recently, we have shown that pomegranate fruit extract (PFE) possesses antitumor promoting effects in a mouse model of chemical carcinogenesis. To begin to establish the effect of PFE for humans in this study, we determined its effect on UV‐B–induced adverse effects in normal human epidermal keratinocytes (NHEK). We first assessed the effect of PFE on UV‐B–mediated phosphorylation of mitogen‐activated protein kinases (MAPK) pathway in NHEK. Immunoblot analysis demonstrated that the treatment of NHEK with PFE (10–40 μg/mL) for 24 h before UV‐B (40 mJ/cm 2 ) exposure dose dependently inhibited UV‐B–mediated phosphorylation of ERK1/2, JNK1/2 and p38 protein. We also observed that PFE (20 μg/mL) inhibited UV‐B–mediated phosphorylation of MAPK in a time‐dependent manner. Furthermore, in dose‐and time‐dependent studies, we evaluated the effect of PFE on UV‐B–mediated activation of nuclear factor kappa B (NF‐κB) pathway. Using Western blot analysis, we found that PFE treatment of NHEK resulted in a dose‐ and time‐dependent inhibition of UV‐B–mediated degradation and phosphorylation of IκBα and activation of IKKα. Using immunoblot analysis, enzyme‐linked immunosorbent assay and electrophoretic mobility shift assay, we found that PFE treatment to NHEK resulted in a dose‐ and time‐dependent inhibition of UV‐B–mediated nuclear translocation and phosphorylation of NF‐κB/p65 at Ser 536 . Taken together, our data shows that PFE protects against the adverse effects of UV‐B radiation by inhibiting UV‐B–induced modulations of NF‐κB and MAPK pathways and provides a molecular basis for the photochemopreventive effects of PFE.
[18F]FPBMP: – a potential new positron emission tomography radioligand for imaging of translocator protein (18 kDa) in peripheral organs of rats (2015)

The five transmembrane translocator protein (18 kDa, TSPO) is abundantly expressed in the mitochondria of activated microglia (brain) and peripheral tissues, including those of the heart, lung and kidney.
Transition‐State Effects of Ionic Liquids in Substitution Reactions of PtII Complexes (2005)

Wenn Normalsein überrascht: Bei der Untersuchung eines Ligandenaustauschs an einem PtII-Komplex (siehe Bild; apa: 2,6-Bis(aminomethyl)pyridin) in Wasser, Methanol und der ionischen Flüssigkeit 1-Butyl-3-methylimidazolium-bis(trifluormethylsulfonyl)amid stellte sich heraus, dass sich die ionische Flüssigkeit „normal“ verhält, d. h. wie jedes andere Solvens. Änderungen in der Polarität des Übergangszustands spielen in ihr nur eine untergeordnete Rolle. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2001/2005/z501329_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Scaling the effective area of higher-order-mode erbium-doped fiber amplifiers (2012)

We demonstrate scaling of the effective area of higher-order mode, Er-doped fiber amplifiers. Two Er-doped higher-order mode fibers, one with 3800 μm(2) A(eff) in the LP(0,11) mode, and one with 6000 μm(2) effective area in the LP(0,14) mode, are demonstrated. Output beam profiles show clean higher order modes, and S(2) imaging measurements show low extraneous higher order mode content. CW and pulsed amplifier experiments are reported. Nanosecond pulses are amplified to 0.5 mJ pulse energy with 0.5 MW peak power.
Conditions for generating scale-invariant density perturbations (2004)

We analyze the general conditions on the equation of state w required for quantum fluctuations of a scalar field to produce a scale-invariant spectrum of density perturbations, including the possibility of models which (in the four dimensional effective description) bounce from a contracting to an expanding phase. We show that there are only two stable cases: $w\ensuremath{\approx}\ensuremath{-}1$ and $w\ensuremath{\gg}1.$ The first is utilized in inflationary models and the second in the ekpyrotic/cyclic scenario, assuming the perturbations propagate through the bounce. All other cases, including the $w\ensuremath{\approx}0$ case considered by some authors, require extreme fine-tuning of initial conditions and/or the effective potential. For the ekpyrotic/cyclic $(w\ensuremath{\gg}1)$ case, we also analyze the small deviations from scale invariance.
A Novel Synthesis of Bicyclic IsoxazolinesviaSequential Michael and Intramolecular 1,3-Dipolar Additions (1987)

Bicyclic isoxazolines are obtained in good yields by the titanium tetrachloride-mediated reaction of allylic stannanes with 1-nitroalkadienes. Titanium tetrachloride converts stannyl nitronates generated in the Michael addition step to nitrile oxide equivalents which, on adding triethylamine, undergo intramolecular 1,3-dipolar cycloaddition to give the isoxazolines.
Weighing nanoparticles and viruses using suspended nanochannel resonators (2011)

We have batch fabricated suspended nanochannel resonators (SNRs) to improve the mass resolution of suspended microchannel resonators (SMRs). The SNR consists of a cantilever that is 50 µm long, 10 µm wide, and 1.3 µm thick, with an embedded nanochannel that is 2 µm wide and 700 nm tall. The SNR has a resonance frequency near 640 kHz and exhibits the maximum quality factor of approximately 16,000 when dry and when filled with water. Using the SNRs, we demonstrated mass measurements of various nanoparticles down to 20 nm in diameter and viruses in solution with a resolution of 27 ag in a 1 kHz bandwidth, which represents a 100-fold improvement over existing SMRs and, to our knowledge, is the most precise mass measurement in liquid today.
Mitochondrial-Derived Peptides Exacerbate Senescence (2018)

Mitochondrial-derived peptides (MDPs), encoded by mitochondrial DNA, play a cytoprotective role by helping preserve mitochondrial function and cell viability under stressful conditions. Humanin and its homologs and MOTS-c are two of several MDPs hypothesized to have antiaging activity based on correlative studies. For example, humanin plasma levels are inversely correlated with growth hormone and insulin-like growth factor 1 expression, which may promote accelerated aging. Humanin has been shown to protect cells from beta amyloid toxicity and preserve endothelial cell function in a mouse model of atherosclerosis. Furthermore, both humanin and MOTS-c improve insulin sensitivity in mouse models of type 2 diabetes. Recently it was reported that a potent analogue of humanin blocks cardiac fibrosis in aging mice. Although it has been hypothesized that MDPs might have senolytic activity, in a recent report humanin and MOTS-c both exacerbate the senescence-associated-secretory-phenotype (SASP) in senescent cells by stimulating the secretion of IL-6, IL-1β, IL-8, IL-10 and tumor necrosis factor α. It appears that the cytoprotective activity of the MDPs may be permissive for increased expression of a set of proinflammatory cytokines. Given the potential benefits of MDPs in many of the same diseases associated with the presence of senescent cells, a combination of senolytic and MDP-based treatments may be additive or synergistic. The MDPs would protect normal cells, whereas senescent cells would be eliminated by the senolytic therapy. It is even possible that MDPs by increasing the SASP phenotype would make the senescent cells more apt to be cleared by the immune system or more sensitive to senolytics. In contrast, if the MDPs actually cytoprotect the senescent cells, then the treatment can be performed serially with the senolytic used first.
19.3: Morphology Controlled Single‐crystal ZnO Nanostructures Fabricated by a Novel Mist Chemical Vapor Deposition (2013)

Abstract A novel method is developed to synthesize single crystal ZnO nanostructures combining the reducing thermal annealing and mist chemical vapor deposition processes. The sizes of ZnO single crystal structures can be controlled with adjusting the parameters in mist CVD. The structural and optical properties of ZnO nanostructures are investigated.
Design limitations for InGaN/AlGaN/GaN lasers imposed by resonant mode coupling (2003)

Summary form only given. Present-day InGaN-AlGaN-GaN semiconductor lasers typically contain a multiple optical waveguide structure, with refractive indices of several layers (GaN buffer-substrate, active region, cap layer) higher than those of cladding layers. This leads to complex behavior of optical modes. Here, we show that resonant effects in nitride lasers result from coupling between nearly-degenerate normal modes of the entire laser structure, similarly to the case of InGaAs-based lasers analyzed previously. We also demonstrate the strong role of the parasitic waveguide thicknesses, in addition to previously investigated parameters.