gebhart/patrepeval huggingface dataset:
Laser-induced fluorescence of flowing samples as an approach to single-molecule detection in liquids (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLaser-induced fluorescence of flowing samples as an approach to single-molecule detection in liquidsNorman J. Dovichi, John C. Martin, James H. Jett, Mitchell. Trkula, and Richard A. KellerCite this: Anal. Chem. 1984, 56, 3, 348–354Publication Date (Print):March 1, 1984Publication History Published online1 May 2002Published inissue 1 March 1984https://pubs.acs.org/doi/10.1021/ac00267a010https://doi.org/10.1021/ac00267a010research-articleACS PublicationsRequest reuse permissionsArticle Views612Altmetric-Citations105LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Dry heating of palm fruits : effect on selected parameters (2010)

Current practice in mill uses steam in fresh fruit bunch sterilization and screw press for oil extraction. Large amount of water used in steam sterilizer ended up as palm oil mill effluent (POME) which requires appropriate treatment. In addition, the POME poses environment concerns through the generation of biogas. Dry heating is believed to be able to reduce, if not eliminatealtogether the usage of water during the milling process. This paper reports the effect of dry heating on oil palm fruitlets. Selected parameters of crude palm oil such as oil yield, deterioration of bleachability Index (DOBl), oxidative stability and free fatty acid (FFA) content were investigated and reported. Preliminary result shows that dry heating at 2-3 minutes is sufficient to halt the lipolysis that leads to the rise in FFA without any damageto the kernel. In addition, the mesocarp can be easily separated from its shell under this heating duration. Although oil yield is found to be highest at 5 minutes of dry heating, the inside kernel is overcooked or damaged. Overall, the quality of oil obtained from oil palm fruits that has undergone dry heating is comparable to that of commercial crude palm oil.
Enhanced thin-film transistor driven high-aperture in-plane switching liquid crystal displays without common line and black matrix (2021)

We developed active-matrix in-plane switching liquid crystal displays (IPS-LCDs) with a new vertical structure composed of thin-film transistors (TFTs) that have an aperture ratio of 60% to reduce energy consumption. The novel TFT has a channel and a back channel made of a hydrogenated amorphous-silicon semiconductor layer sandwiched by thin silicon oxide insulating layers. The transfer characteristics are enhanced by uniformly shifting the threshold voltage to be higher than the maximum LC driving voltage (typically > 5 V). The enhanced TFT characteristics provided with a new driving scheme and shielding electrodes enables both the common line and black matrix to be eliminated. We fabricated an IPS TFT-LCD panel with aperture and contrast ratios that are 160% those of the conventional pixel structure.
Is vacuum decay significant in ekpyrotic and cyclic models? (2002)

It has recently been argued that bubble nucleation in ekpyrotic and cyclic cosmological scenarios can lead to unacceptable inhomogeneities unless certain constraints are satisfied. In this paper we show that this is not the case. We find that bubble nucleation is completely negligible in realistic models.
Pleiotropic tumor suppressor functions of WWOX antagonize metastasis (2020)

Tumor progression and metastasis are the major causes of death among cancer associated mortality. Metastatic cells acquire features of migration and invasion and usually undergo epithelia-mesenchymal transition (EMT). Acquirement of these various hallmarks rely on different cellular pathways, including TGF-β and Wnt signaling. Recently, we reported that WW domain-containing oxidoreductase (WWOX) acts as a tumor suppressor and has anti-metastatic activities involving regulation of several key microRNAs (miRNAs) in triple-negative breast cancer (TNBC). Here, we report that WWOX restoration in highly metastatic MDA-MB435S cancer cells alters mRNA expression profiles; further, WWOX interacts with various proteins to exert its tumor suppressor function. Careful alignment and analysis of gene and miRNA expression in these cells revealed profound changes in cellular pathways mediating adhesion, invasion and motility. We further demonstrate that WWOX, through regulation of miR-146a levels, regulates SMAD3, which is a member of the TGF-β signaling pathway. Moreover, proteomic analysis of WWOX partners revealed regulation of the Wnt-signaling activation through physical interaction with Disheveled. Altogether, these findings underscore a significant role for WWOX in antagonizing metastasis, further highlighting its role and therapeutic potential in suppressing tumor progression.
Supplementary Tables and Figures from Association of Common Susceptibility Variants of Pancreatic Cancer in Higher-Risk Patients: A PACGENE Study (2023)

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Localization of luminescent inhomogeneities in turbid media with spatially resolved measurements of cw diffuse luminescence emittance (1998)

We present a steady-state method for localizing a source ofluminescence (i.e., fluorescence or phosphorescence) buried in asemi-infinite turbid medium with unknown optical properties. Adiffusion theory expression describing the emittance of an isotropicpoint source is fit to spatially resolved surface measurements of thediffuse emittance from the luminescent source. The techniquereports the location of the center of a 6.0-mm-diameter, fluorophore-containing spherical bulb embedded in a liquid phantom withan accuracy of 1.0 mm or better for source depths as great as 40.0mm. Monte Carlo data are analyzed to investigate the range and thepossible sources of error in the reconstructed source depth.
Precoding with X-codes to increase capacity with discrete input alphabets (2010)

We consider Gaussian multiple-input multiple-output (MIMO) channels with discrete input alphabets. We propose a non-diagonal precoder based on X-Codes in to increase the mutual information. The MIMO channel is transformed into a set of parallel subchannels using Singular Value Decomposition (SVD) and X-codes are then used to pair the subchannels. X-Codes are fully characterized by the pairings and the 2 × 2 real rotation matrices for each pair (parameterized with a single angle). This precoding structure enables to express the total mutual information as a sum of the mutual information of all the pairs. The problem of finding the optimal precoder with the above structure, which maximizes the total mutual information, is equivalent to i) optimizing the rotation angle and the power allocation within each pair and ii) finding the optimal pairing and power allocation among the pairs. It is shown that the mutual information achieved with the proposed pairing scheme is very close to that achieved with the optimal precoder by Cruz et al., and significantly better than mercury/waterfilling strategy by Lozano et al.. Our approach greatly simplifies both the precoder optimization and the detection complexity, making it suitable for practical applications.
High Sensitivity of Shotgun Metagenomic Sequencing in Colon Tissue Biopsy by Host DNA Depletion (2022)

The high host genetic background of tissue biopsies hinders the application of shotgun metagenomic sequencing in characterizing the tissue microbiota. We proposed an optimized method that removed host DNA from colon biopsies and examined the effect on metagenomic analysis. Human or mouse colon biopsies were divided into two groups, with one group undergoing host DNA depletion and the other serving as the control. Host DNA was removed through differential lysis of mammalian and bacterial cells before sequencing. The impact of host DNA depletion on microbiota was compared based on phylogenetic diversity analyses and regression analyses. Removing host DNA enhanced bacterial sequencing depth and improved species discovery, increasing bacterial reads by 2.46 ± 0.20 folds while reducing host reads by 6.80% ± 1.06%. Moreover, 2.40 times more of bacterial species were detected after host DNA depletion. This was confirmed from mouse colon tissues, increasing bacterial reads by 5.46 ± 0.42 folds while decreasing host reads by 10.2% ± 0.83%. Similarly, significantly more bacterial species were detected in the mouse colon tissue upon host DNA depletion (P < 0.001). Furthermore, an increased microbial richness was evident in the host DNA-depleted samples compared with non-depleted controls in human colon biopsies and mouse colon tissues (P < 0.001). Our optimized method of host DNA depletion improves the sensitivity of shotgun metagenomic sequencing in bacteria detection in the biopsy, which may yield a more accurate taxonomic profile of the tissue microbiota and identify bacteria that are important for disease initiation or progression.
Effects of surface pre-reacted glass-ionomer (S-PRG) eluate on Candida spp.: antifungal activity, anti-biofilm properties, and protective effects on Galleria mellonella against C. albicans infection (2019)

Surface pre-reacted glass-ionomer (S-PRG) is a bioactive filler produced by PRG technology, which is applied to various dental materials. The inhibitory effects of S-PRG eluate against <i>Candida</i>, the most common fungal oral pathogen, were investigated. Minimum inhibitory concentrations (MIC) and anti-biofilm activities were tested against <i>Candida albicans</i>, <i>Candida glabrata</i>, <i>Candida krusei</i>, and <i>Candida tropicalis</i>. For the <i>in vivo</i> study, <i>Galleria mellonella</i> was used as a model to evaluate the effects of S-PRG on toxicity, hemocyte counts and candidiasis. The MIC of S-PRG ranged from 5 to 40% (v/v). S-PRG eluate exhibited anti-biofilm activity for all the <i>Candida</i> species tested. Furthermore, injection of S-PRG eluate into <i>G. mellonella</i> was not toxic to the larvae and protected <i>G. mellonella</i> against experimental candidiasis. In addition, S-PRG eluate inhibited biofilm formation by <i>C. albicans</i>, <i>C. glabrata</i>, <i>C. krusei</i>, and <i>C. tropicalis</i> and exerted protective effects on <i>G. mellonella</i> against experimental candidiasis <i>in vivo</i>.
Enzyme diffusion and action on soluble and insoluble substrate biopolymers (1985)

Abstract The action of enzymes on soluble and insoluble substrate biopolymers is discussed, taking into account enzyme diffusion along the biopolymer “surface” and interaction with interspersed ligand groups that may be modified by the action of the enzyme. It is shown that movement of the enzyme under trhe combined effect of these two processes can be described as a diffusion process characterized by an apparent diffusion coefficient that generally depends on both time and position. Equations describing the system are formulated and some specific examples analyzed in terms of analytical or numerical solutions. The concentration distributions of both the enzyme and the substrate (or product ) were obtained for different systems for which the apparent diffusion coefficient is a function of time only, as well as of both time and position. The relevance of the formulation, as developed, to systems in which reduction in dimensionality leads to enhanced enzyme efficiency is discussed, and possible uses of the theory in studies of biopolymer structure and enzyme‐biopolymer interactions are suggested.
Retrovirus D/New England and its relation to Mason-Pfizer monkey virus (1985)

Seventeen isolates of retrovirus D/New England have been obtained from three species of macaques at the New England Regional Primate Research Center. Seven of the isolates were obtained from macaques who subsequently died with the macaque immunodeficiency syndrome; other isolates were obtained from macaques with less severe or other forms of illness. Attempts to isolate type D retrovirus from peripheral lymphocytes of 97 apparently healthy macaques have not been successful. Cloned DNA was prepared from Hirt supernatants of cells infected with one of these isolates (D/New England 398). By restriction endonuclease analysis, cloned pD398 DNA represented full-length viral DNA with one long terminal repeat. A detailed restriction endonuclease map of pD398 was derived and compared with a map of the cloned Mason-Pfizer monkey virus genome. Forty-six percent (13 of 28) of restriction endonuclease sites were found to be conserved when these related viruses were compared. Five of the D/New England isolates, including those from three different macaque species, were examined for strain variability by restriction endonuclease typing. Comparison of over 30 restriction endonuclease sites has not distinguished any of these D/New England isolates. It thus appears that a single strain of type D retrovirus is infecting three different species of macaques in the New England colony. Markedly reduced cross-hybridization was observed between cloned pD398 and Mason-Pfizer monkey virus DNAs at high stringency; this reduced cross-hybridization was localized to the pol-env regions of the genome. Only very weak hybridization of D/New England DNA to cloned squirrel monkey type D retrovirus DNA could be detected even at low-stringency conditions. What role type D retrovirus plays in the immunodeficiency syndrome of macaques remains to be determined.
Quantification of Sesquiterpene Lactones in Parthenium hyterophorous by Normal-Phase HPLC (2013)

This paper describes the development of a normal-phase liquid chromatography ultraviolet-diode array detection method for the simultaneous quantification of parthenin and coronopilin in the leaves and flowers of Parthenium hysterophorous. The compounds were analyzed on a Merck Si60 silica column (5 µm, 250 × 4 mm) using an isocratic 15:85 mixture of isopropyl alcohol and hexane. The calibration curves resulting from the reference compounds in the concentration range of 200-2,000 ng exhibited acceptable linearity (r > 0.999). The method was developed to study the levels of parthenin and coronopilin in the leaves and flowers of P. hysterophorous collected during different seasons, and the method was validated by analyzing the spiked samples.
Anthracene-triazole-dicarboxylate-Based Zn(II) 2D Metal Organic Frameworks for Efficient Catalytic Carbon Dioxide Fixation into Cyclic Carbonates under Solvent-Free Condition and Theoretical Study for the Reaction Mechanism (2021)

Two ZnII-based metal organic frameworks (MOFs) [Zn2(L)2(1,2-bis(4-pyridyl)ethane)4]n [(ZnMOF1)] and [Zn2(L)2(1,2-bis(4-pyridyl)ethene)4]n [(ZnMOF2)] have been synthesized and crystallographically characterized. Due to the presence of Lewis acidic center Zn and basic functional groups (−N═N) in both complex [(ZnMOF1)] and [(ZnMOF2)], it is possible to efficiently fix carbon dioxide into epoxides to produce biosource cyclic carbonates. Under solvent-free and mild reaction conditions (60 °C temperature and 1 bar CO2 pressure), various types of epoxides (terminal as well as internal epoxides) effectively undergo this catalytic reaction and produced high yield of the corresponding cyclic carbonate products. High turnover frequency was obtained for this CO2 fixation reaction of different epoxides, and the values are in the range of 72–234 h–1. The theoretical calculations are made by density functional theory (DFT) theory to achieve the relative energy of the intermediates and transition state and product which are engaged in each step of the catalytic reaction cycle, and the detailed mechanism of the reaction is presented. Moreover, the two MOF catalysts are easily recoverable and recyclable in nature. The efficiencies of both catalysts regain even after seven consecutive catalytic cycles. Overall, the work demonstrates the design and syntheses of two Zn(II)MOF catalysts for effective addition of CO2 into epoxides to produce cyclic carbonates and DFT study for the catalytic reaction mechanism.
INFLUENCE OF FORMING IN THE T3 CONDITION ON PROPERTIES OF 2090-T8X, 2091-T8X AND 8090-T8X (1987)

A forming sequence for Al-Li alloys in which T3 material is aged directly to T8 without an intervening solution treatment would be desirable. Such a sequence would allow forming in a relatively formable condition, provide the deformation necessary for highest strength, and eliminate quenching distortion problems. In order to evaluate the feasibility of such a sequence, we have subjected samples of 2090-T3E27, 2091-T3 and 8090-T3 to various amounts of stretching, aged them for various lengths of time and measured their tensile strength and corrosion resistance, and characterized their precipitate microstructures. The results of these tests show some differences in response between the alloys but indicate that the proposed sequence is feasible.
Corrigendum to: 2021 ESC Guidelines on cardiac pacing and cardiac resynchronization therapy: Developed by the Task Force on cardiac pacing and cardiac resynchronization therapy of the European Society of Cardiology (ESC): With the special contribution of the European Heart Rhythm Association (EHRA) (2022)

In the originally published version of thismanuscript, there were several errors which are listed in this corrigendumas follows: In Table 4, The Level of Evidence for "For venous access, the cephalic or axillary vein should be considered as first choice" should read "B" instead of "C". In Table 10, the wording "TAVI in valve in valve vs. native valve procedure" should read "TAVI in native valve vs. valve-in-valve procedure". In the Supplementary Data, the wording in Table 14 should read "TAVI in native valve vs. valve-in-valve procedure" instead of "TAVI in valve in valve vs. native valve procedure". These errors have now been corrected.
Application of a multi‐gene next‐generation sequencing panel to a non‐invasive oesophageal cell‐sampling device to diagnose dysplastic Barrett's oesophagus (2017)

The early detection and endoscopic treatment of patients with the dysplastic stage of Barrett's oesophagus is a key to preventing progression to oesophageal adenocarcinoma. However, endoscopic surveillance protocols are hampered by the invasiveness of repeat endoscopy, sampling bias, and a subjective histopathological diagnosis of dysplasia. In this case-control study, we investigated the use of a non-invasive, pan-oesophageal cell-sampling device, the Cytosponge™, coupled with a cancer hot-spot panel to identify patients with dysplastic Barrett's oesophagus. Formalin-fixed, paraffin-embedded (FFPE) Cytosponge™ samples from 31 patients with non-dysplastic and 28 with dysplastic Barrett's oesophagus with good available clinical annotation were selected for inclusion. Samples were microdissected and amplicon sequencing performed using a panel covering <i>></i>2800 COSMIC hot-spot mutations in 50 oncogenes and tumour suppressor genes<i>. S</i>trict mutation criteria were determined and duplicates were run to confirm any mutations with an allele frequency <12%. When compared with endoscopy and biopsy as the gold standard the panel achieved a 71.4% sensitivity (95% CI 51.3-86.8) and 90.3% (95% CI 74.3-98.0) specificity for diagnosing dysplasia. <i>TP53</i> had the highest rate of mutation in 14/28 dysplastic samples (50%). <i>CDKN2A</i> was mutated in 6/28 (21.4%), ERBB2 in 3/28 (10.7%), and 5 other genes at lower frequency. The only gene from this panel found to be mutated in the non-dysplastic cases was <i>CDKN2A</i> in 3/31 cases (9.7%) in keeping with its known loss early in the natural history of the disease. Hence, it is possible to apply a multi-gene cancer hot-spot panel and next-generation sequencing to microdissected, FFPE samples collected by the Cytosponge™, in order to distinguish non-dysplastic from dysplastic Barrett's oesophagus. Further work is required to maximize the panel sensitivity.
On the Formation of Tetracoordinate Boron in Rubidium Borate Glasses (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOn the Formation of Tetracoordinate Boron in Rubidium Borate GlassesR. E. Youngman and J. W. ZwanzigerCite this: J. Am. Chem. Soc. 1995, 117, 4, 1397–1402Publication Date (Print):February 1, 1995Publication History Published online1 May 2002Published inissue 1 February 1995https://pubs.acs.org/doi/10.1021/ja00109a026https://doi.org/10.1021/ja00109a026research-articleACS PublicationsRequest reuse permissionsArticle Views280Altmetric-Citations42LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Quantitative modeling of public local area access value network configurations (2011)

The growing amount of mobile data traffic increases the demand for local area network deployments and is therefore likely to have an effect on the mobile communications ecosystem. Several alternative value network configurations exist giving new business opportunities to different actors, but the attractiveness and the success of local area access provisioning remains uncertain. This study analyzes the business of public local area access from the point of view of two potential providers: mobile operators and service application providers. A quantitative techno-economic model is constructed to quantify the costs and required revenues of operating public LA access networks. The results show that the revenue requirements vary between public LA venue types and value network configurations.