gebhart/patrepeval huggingface dataset:
IFN- gamma -regulated expression of a differentiation antigen of human cells. (1988)

Mouse mAb M111 identifies a cell surface glycoprotein of 115,000 to 135,000 Da. M111 was expressed constitutively in subsets of cells of multiple lineages at discrete stages of cell maturation, suggesting that M111 is a differentiation Ag of the three germ layers. Ag expression could be induced by IFN-gamma but not by IFN-alpha, IFN-beta, or TNF. Induction of M111 expression was maximal at 48 h of culture in 200 U/ml of IFN-gamma and was independent of induction of class II MHC Ag. Induction was dependent on the cell type used. Nine colon cancer cell lines of undifferentiated phenotype were constitutively M111-; IFN-gamma induced M111 expression in seven of them. In contrast, IFN-gamma failed to induce M111 expression in six of six M111- ovarian cancer cell lines. Eight normal fibroblast cultures tested were M111-; they could not be induced to express M111. Three of five sarcoma cell lines were M111+; culture in IFN-gamma induced an increase in M111 expression in all of them. Constitutive and IFN-gamma-induced expression of M111 was independent of constitutive and induced expression of HLA class I and II molecules. IFN-gamma-mediated induction of M111 expression was not accompanied by coordinate changes in the expression of other differentiation traits. These results suggest that expression of the M111 gene is controlled by two mechanisms, one related to differentiation and the other activated by IFN-gamma.
Principles and Practice of Intensive Care Monitoring. (1999)

Adjunct Professor of AnesthesiologyJohns Hopkins University School of MedicineBaltimore, MarylandPrinciples and Practice of Intensive Care Monitoring. Edited by Martin J. Tobin. New York, McGraw-Hill, 1998. Pages: 1200. Cost:$140.00.The past decade has seen multiple textbooks on monitoring, usually smallish mes that provide detailed information on common monitoring procedures and their underlying technology. The new textbook, Principles and Practice of Intensive Care Monitoring, takes a much broader view of monitoring, including all forms of monitoring used in the intensive care unit and focusing on how physiologic data can be used to modify patient outcomes. The book contains detailed reviews of most of the available methods for obtaining physiologic data from patients in the intensive care unit. Many of these methods are in common use, whereas others are state-of-the-art procedures used in only a few specialized centers. Much attention is devoted to analyses examining the accuracy (and usefulness) of the data generated by these devices and to how physicians and other care givers can use this information to manage complex patients in the intensive care unit. Chapters are grouped together by organ system, with respiratory and cardiovascular monitors receiving the most attention. There are also chapters dealing with specific situations (e.g. , transport) and technologies (e.g. , molecular biologic tests). Topics range from the very technical (engineering data on alarms—their frequency, pitch, and intensity) to the purely clinical (indications for placing a pulmonary artery catheter). Taken as a whole, Tobin has created a unique book—one that redefines what monitoring is all about.The book is large and expansive and is aimed at the intensivist who wishes to use technology wisely and provide high-quality patient care. Although certain chapters can serve as reference standards (e.g. , techniques to measure respiratory muscle strength), the book can also function as a series of interesting and useful reviews to be read at one's leisure. The chapters addressing the potential utility of computers in medicine are particularly interesting, providing insight both into the potential offered by this technology and the daunting hurdles that must be circumvented to translate this potential into useful clinical tools.The authors are leaders in their fields. The overall quality of writing is high. All forms of monitoring modalities are covered. Although one can quibble that some topics are covered in excessive depth (in comparison to the whole) and a few chapters fail to deliver a clear message, the balance is excellent, and there is minimal overlap between chapters.In summary, this is an excellent textbook and well worth the price. Intensivists will find it both stimulating and useful. It belongs in the library of all major hospitals and medical schools.
Rational Color Tuning and Luminescent Properties of Functionalized Boron‐Containing 2‐Pyridyl Pyrrolide Complexes (2004)

Abstract Three systematically functionalized pyrrolide ligands were prepared via the coupling of methyl vinyl ketone and the respective carbaldehyde reagents, followed by treatment of the pre‐formed dicarbonyl compounds with (NH 4 ) 2 CO 3 in order to generate the required pyrrole fragment. These ligands readily reacted with the boron reagent BPh 3 to afford the complexes [(pyro)BPh 2 ] ( 2a ), [(noro)BPh 2 ] ( 2b ), and [(xaro)BPh 2 ] ( 2c ), where (pyro)H, (noro)H, and (xaro)H represents the 2‐pyridyl, 2‐quinolinyl, and 2‐quinoxalinyl pyrrole groups, respectively. Complexes 2a – 2c give stable solutions in air, and show strong photoluminescence with emission peak maxima located at 490 nm, 510 nm, and 575 nm, respectively. Calculations based upon time‐dependent density function theory (TDDFT) show that the S 1 state in these complexes is attributed to an allowed (π‐symmetry) → π* (π‐symmetry) transition located at the chelating pyrrolide moieties. Electroluminescence (EL) devices based on 2c were fabricated. The EL emission from 2c as the host‐emitter, with the emission peak maximum shifted to 580 nm, was observed when BCP was used as the hole blocking material. This device produces saturated red‐orange light‐emission at an onset voltage of 8 V and a maximum brightness of 5000 cd m –2 at a driving voltage of 15 V; the external quantum yield is estimated to be 0.5 %.
A comparison of the effects of human and ovine corticotropin-releasing hormone on the pituitary-adrenal axis. (1995)

To compare the clinical efficacy of ovine and human sequence corticotropin-releasing hormone (CRH), we examined the effects of both peptides on ACTH and cortisol secretion in normal human volunteers, obese subjects, and patients with pituitary-dependent (Cushing's disease) and adrenal-dependent Cushing's syndrome. All subjects in each group were studied twice in random order. One hundred micrograms of CRH were administered as an iv bolus through an in-dwelling forearm cannula at 0930 h, and thereafter, blood was drawn every 15 min for 2 h for the measurement of ACTH and cortisol. In the normal subjects, the peak ACTH, peak incremental ACTH, and mean area under the curve after CRH treatment were greater with ovine CRH than human CRH, although there was no difference in the cortisol response, however it was analyzed. There was no difference in the ACTH or cortisol response to the two preparations in the obese subjects, and no significant difference was found, for either cortisol or ACTH, between obese subjects and normal volunteers. With both varieties of CRH, Cushing's disease resulted in greater responses for ACTH and cortisol than those seen in the other 2 groups (P < 0.001 for all comparisons), but there was no difference between the sequences. However, a significant cortisol response, defined as an increase of greater than 4 times the coefficient of variation of the assay (24%), was seen in all 10 Cushing's patients with human CRH, but in only 8 with ovine CRH. In 3 patients with adrenal tumors, serum cortisol did not change after the administration of either preparation, whereas plasma ACTH remained undetectable throughout the study. We suggest that although ovine sequence CRH causes more prolonged and greater ACTH, and possibly cortisol, secretion compared to human CRH, the discriminatory value of the CRH test, in terms of either the diagnosis or differential diagnosis of Cushing's syndrome, is comparable for the two peptide sequences.
A design approach to fully compliant multistable mechanisms employing a single bistable mechanism (2020)

A fully compliant multistable mechanism is a monolithic structure that is capable of staying at multiple positions without power input, and has many applications including switches, valves, positioners. However, it is difficult to design such a mechanism because of the complexities of the multistable behavior, the practical stress limits and the buckling constraints. This paper discusses the design approach for fully compliant multistable mechanisms which employs a single bistable mechanism and several end-effectors connected in series. The force-displacement characteristics of the end-effectors are derived using the pseudo-rigid-body model. The design approach to the fully compliant multistable mechanism is provided to determine the design parameters for the flexible segments considering the critical buckling load and the bending strength. Two design examples are presented to demonstrate the feasibility of the approach.
Abstract NG02: NFS1 undergoes positive selection in lung tumors and protects cells from ferroptosis (2018)

Abstract Environmental nutrient levels impact cancer cell metabolism, resulting in context-dependent gene essentiality. Here, using RNAi-based loss of function screening, we identify environmental oxygen level as a major driver of differential essentiality between in vitro model systems and in vivo tumours. Above the 3-8% oxygen concentration typical of most tissues, we find that cancer cells depend on high levels of the iron-sulfur cluster (ISC) biosynthetic enzyme NFS1. Accordingly, mammary or subcutaneous tumours grow despite NFS1 suppression, while metastatic or primary lung tumours do not. Consistent with a role in surviving the high oxygen environment of incipient lung tumours, NFS1 lies in a region of genomic amplification present in lung adenocarcinoma and is most highly expressed in well-differentiated adenocarcinomas. NFS1 activity is particularly important for maintaining the ISC cofactors present in multiple cell-essential proteins upon exposure to O2 compared to other forms of oxidative damage. Additionally, insufficient ISC maintenance robustly activates the iron-starvation response and, in combination with glutathione biosynthesis inhibition, triggers ferroptosis, a non-apoptotic form of cell death. Suppression of NFS1 cooperates with inhibition of cysteine transport to trigger ferroptosis in vitro and slow tumour growth. Therefore, lung adenocarcinomas select for expression of a pathway that confers resistance to high oxygen tension and protects cells from undergoing ferroptosis in response to oxidative damage. These observations lead to the tantalizing hypothesis that one can trick cancer cells into taking up large quantities of iron and releasing intracellular iron stores via modulation of NFS1 or downstream effectors, such as IRPs, leaving them at increased risk for ROS-mediated cell death mechanisms such as ferroptosis. Citation Format: Samantha W. Alvarez, Vladislav O. Sviderskiy, Erdem M. Terzi, Thales Papagiannakopoulos, Andre L. Moreira, Sylvia Adams, David M. Sabatini, Kıvanç Birsoy, Richard L. Possemato. NFS1 undergoes positive selection in lung tumors and protects cells from ferroptosis [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr NG02.
A Simple and Fast Converging Algorithm for MMSE Adaptive Array Antenna (2000)

This paper proposes a fast and simple adaptive algorithm for MMSE (Minimum Mean Square Error) adaptive array antenna or MMSE combining diversity. This algorithm can be implemented with as a small operation as LMS since it is based on VS-LMS (variable step size LMS) for which the step size is provided with a fixed profile. Computer simulation results show that the proposed algorithm has much better convergence behavior and BER performance than LMS.
Physical Layer Techniques for 5G Wireless Security (2018)

Wireless communications and cellular networks are vulnerable to various security threats due to several reasons. Physical layer techniques and technologies, such as massive multiple-input multiple-output (MIMO), millimeter communications, cognitive radio, and full-duplex wireless, can be easily integrated to implement comprehensive 5G wireless security solutions. This chapter discusses physical layer security techniques for 5G wireless communications. It introduces full-duplex communications and transceiver architecture, and discusses prior art on full-duplex physical layer security schemes reported for the bidirectional, base station, and relay topologies. The chapter considers the optimum beamforming designs for full duplex wireless security solutions for multiantenna bidirectional and relay communication systems. It emphasizes future challenges and open issues for the implementation of full duplex secure transmissions in 5G systems. Due to simultaneous transmissions, full-duplex operation in cellular networks can introduce increased levels of co-channel interference.
Magnetic Resonance Elastography of Liver in Light Chain Amyloidosis (2019)

In this paper, we present our preliminary findings regarding magnetic resonance elastography (MRE) on the livers of 10 patients with systemic amyloidosis. Mean liver stiffness measurements (LSM) and spleen stiffness measurements (SSM) were obtained. Magnetic resonance imaging (MRI) images were analyzed for the distribution pattern of amyloid deposition. Pearson correlation analysis was performed in order to study the correlation between LSM, SSM, liver span, liver volume, spleen span, spleen volume, serum alkaline phosphatase (ALP), N-terminal pro b-type natriuretic peptide (NT pro BNP), and the kappa and lambda free light chains. An increase in mean LSM was seen in all patients. Pearson correlation analysis showed a statistically significant correlation between LSM and liver volume (<i>r</i> = 0.78, <i>p</i> = 0.007) and kappa chain level (<i>r</i> = 0.65, <i>p</i> = 0.04). Interestingly, LSM did not correlate significantly with SSM (<i>r</i> = 0.45, <i>p</i> = 0.18), liver span (<i>r</i> = 0.57, <i>p</i> = 0.08), or serum ALP (<i>r</i> = 0.60, <i>p</i> = 0.07). However, LSM correlated significantly with serum ALP when corrected for liver volume (partial correlation, <i>r</i> = 0.71, <i>p</i> = 0.03) and NT pro BNP levels (partial correlation, <i>r</i> = 0.68, <i>p</i> = 0.04). MRI review revealed that amyloid deposition in the liver can be diffuse, lobar, or focal. MRE is useful for the evaluation of hepatic amyloidosis and shows increased stiffness in hepatic amyloidosis. MRE has the potential to be a non-invasive quantitative imaging marker for hepatic amyloidosis.
Phase 1, first-in-human, open label, dose escalation ctudy of MGD009, a humanized B7-H3 x CD3 dual-affinity re-targeting (DART) protein in patients with B7-H3-expressing neoplasms or B7-H3 expressing tumor vasculature. (2016)

TPS3105 Background: MGD009 is a B7-H3 x CD3 Dual Affinity Re-Targeting (DART) protein. DART proteins are bispecific, antibody-based molecules that can bind two distinct antigens simultaneously. MGD009 is designed to redirect T cells to eliminate B7-H3-expressing target cells through co-engagement of B7-H3 on the target cell and CD3 on the T cell, each in a monovalent binding manner. MGD009 contains a mutated human IgG1 Fc domain to reduce / eliminate effector function via binding to Fc gamma receptors (FcγRs) and complement, but retains binding to the neonatal Fc receptor (FcRn) enabling use of the IgG salvage pathway to prolong circulating its half-life. B7-H3 protein expression is extremely limited in normal human tissues but is overexpressed in a broad range of tumor types, including melanoma (M), NSCLC (LC), SCCHN (HN), mesothelioma (MESO) and urothelial cancer (UC). By binding to B7-H3 on tumor cells and CD3 on T lymphocytes, MGD009 can recruit cytotoxic T cells, irrespective of their ability to recognize cancer cells, and trigger T-cell activation, expansion, and T-cell mediated killing of B7-H3- expressing tumors. The limited expression of B7-H3 on normal cells should restrict the potential for cytolytic activity directed at normal tissues, and unintended toxicity in patients treated with MGD009. Methods: This multi-center, open-label trial is a Phase 1 dose escalation / cohort expansion study (NCT02628535). All patients must have advanced B7-H3+ tumors. Prior checkpoint inhibitor therapy will be allowed. Dose escalation uses a 3+3+3 design, with patients treated every 2 weeks with escalating doses of IV MGD009 (starting dose 0.3 ug/kg). Cohort expansions (N = 16/cohort) treated at the maximum tolerated dose will include patients with M, HN, MESO, UC and LC. Major objectives include characterization of the safety, pharmacokinetics, pharmacodynamics, and preliminary antitumor activity of MGD009 in this ongoing study. Clinical trial information: NCT02628535.
3D grating-based X-ray phase-contrast computed tomography for high-resolution quantitative assessment of cartilage: An experimental feasibility study with 3T MRI, 7T MRI and biomechanical correlation (2019)

In conclusion, high-resolution grating-based X-ray PCCT cartilage imaging is feasible. In addition to MRI and biomechanical analyses it provides complementary, water content independent, information for improved morphological and quantitative characterization of articular cartilage ultrastructure.
Development of an Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Ringworm Infection in Cattle (2013)

The aim of this study was to develop an in-house enzyme-linked immunosorbent assay (ELISA) for the serological diagnosis of ringworm infection in cattle. We used available recombinant forms of Trichophyton rubrum dipeptidyl peptidase V (TruDppV) and T. rubrum leucin aminopeptidase 2 (TruLap2), which are 98% identical to Trichophyton verrucosum orthologues. Field serum samples from 135 cattle with ringworm infection, as confirmed by direct microscopy, fluorescence microscopy, and PCR, and from 55 cattle without any apparent skin lesions or history of ringworm infection that served as negative controls were used. Sensitivities, specificities, and positive and negative predictive values were determined to evaluate the diagnostic value of our ELISA. Overall, the ELISAs based on recombinant TruDppV and TruLap2 discriminated well between infected animals and healthy controls. Highly significant differences (P < 0.0001, Mann-Whitney U test) were noted between optical density values obtained when sera from infected versus control cattle were tested. The ELISA developed for the detection of specific antibodies against DppV gave 89.6% sensitivity, 92.7% specificity, a 96.8% positive predictive value, and a 78.4% negative predictive value. The recombinant TruLap2-based ELISA displayed 88.1% sensitivity, 90.9% specificity, a 95.9% positive predictive value, and a 75.7% negative predictive value. To the best of our knowledge, this is the first ELISA based on recombinant antigens for assessing immune responses to ringworm infection in cattle; it is particularly suitable for epidemiological studies and also for the evaluation of vaccines and/or vaccination procedures.
Long travel range thermal actuator for deeply etched MEMS components (2018)

In this work, we report a long travel range thermal actuator for deeply-etched optical MEMS applications. Optimized biasing and mechanical architecture is used to reduce the power consumption and maximize the generated deflection. The design is simulated and optimized using COMSOL Multi-physics 5.1 coupling the electrical, the thermal and the mechanical simulation in a single platform. A linear displacement of about 540 μm could be achieved for an etching depth of 100 μm and an applied voltage of 90 V. The rise time of the motion is about 20 ms to rise from 10 % to 90 % of the steady state displacement.
Linking Phase Behavior to Performance Parameters in Non‐Fullerene Acceptor Solar Cells (2023)

Abstract Performance of nonfullerene‐based organic solar cells is largely dependent on the donor:acceptor blend morphology, which is initially tuned by composition. However, morphological design rules are difficult to identify due to the complex relationship between composition‐dependent phase behavior and performance properties. In this study, the authors are able to establish a direct link between PM6:Y6 film morphology and device properties by combining grazing‐incidence wide‐angle X‐ray scattering with highly sensitive Fourier transform photocurrent spectroscopy. By analyzing properties across the full composition range, interfacial microstructure and the corresponding charge transfer (CT) states are identified and direct structure–property relationships are established. The results indicate that open‐circuit voltage is controlled by the CT state at amorphous‐PM6:crystalline‐Y6 interfaces. Crystalline Y6 is found to increase both transport and non‐radiative recombination, suggesting that morphological tradeoffs may be necessary for overall device optimization.
Placement of Biological Membrane Patches in a Nanofluidic Gap With Control Over Position and Orientation (2022)

Abstract Purple membranes from the archaeon Halobacterium salinarum consist of 2D crystals of the light‐driven proton pump bacteriorhodopsin, which convert photons into a proton gradient across the cell membrane. This functional feature and the structural rigidity make them appealing candidates for integration into biomimetic devices. To this end, and in order to carry out their function, purple membranes must be positioned in the correct orientation at the position of interest. Precise placement and control over the orientation of nanoscale objects still constitutes a formidable challenge. Here, it is shown that isolated purple membrane patches can be transported and positioned at predefined locations in nanofluidic confinement, with control over their orientation at the target sites. The transport is achieved through a rocking Brownian motor scheme, while the controlled deposition of the membranes is realized by engineering the surface potential of a fluid‐filled nanofluidic slit. This controlled manipulation of purple membrane patches outlines a new pathway toward the integration of biological or other delicate supramolecular structures into top–down‐fabricated patterns, for the assembly of nanoscale hybrid devices that serve as a light‐driven source of (chemical) energy.