gebhart/patrepeval huggingface dataset:
The yeast cell fusion protein FUS1 is O-glycosylated and spans the plasma membrane. (1989)

Previous work has shown that efficient cell fusion during conjugation in Saccharomyces cerevisiae requires a pheromone-induced surface protein encoded by FUS1. We show that the FUS1 protein migrates on SDS/polyacrylamide gels with an apparent molecular mass of 80 kDa, although the mass is predicted to be 58 kDa from the gene coding capacity. This discrepancy results from the presence of O-linked mannose oligosaccharides attached to the clustered serines and threonines at the amino terminus of the protein. The addition of mannose is completely abolished in the early secretory mutant sec53, attenuated in the late-endoplasmic reticulum-blocked sec18, and unaffected in sec7, which is blocked late in the Golgi phase of secretion. Membrane fractionation and protease protection experiments indicate that FUS1 spans the plasma membrane, with its glycosylated amino terminus projecting into the periplasmic space.
An Overview of the IBM Power 775 Supercomputer Water Cooling System (2012)

In 2008 IBM reintroduced water cooling technology into its high performance computing platform, the Power 575 Supercomputing node/system. Water cooled cold plates were used to cool the processor modules which represented about half of the total system (rack) heat load. An air-to-liquid heat exchanger was also mounted in the rear door of the rack to remove a significant fraction of the other half of the rack heat load: the heat load to air. The next generation of this platform, the Power 775 Supercomputing node/system, is a monumental leap forward in computing performance and energy efficiency. The computer node and system were designed from the start with water cooling in mind. The result, a system with greater than 96% of its heat load conducted directly to water, is a system that, together with a rear door heat exchanger, removes 100% of its heat load to water with no requirement for room air conditioning. In addition to the processor, the memory, power conversion, and I/O electronics conduct their heat to water. Included within the framework of the system is a disk storage unit (disc enclosure) containing an interboard air-to-water heat exchanger. This paper will give an overview of the water cooling system featuring the water conditioning unit and rack manifolds. Advances in technology over this system’s predecessor will be highlighted. An overview of the cooling assemblies within the server drawer (i.e., central electronics complex,) the disc enclosure, and the centralized (bulk) power conversion system will also be given. Furthermore, techniques to enhance performance and energy efficiency will also be described.
ChemInform Abstract: Directed Methallylations as a Synthetic Route to 1,3‐Polyols. (1998)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Feasibility of real-time treatment feedback using novel filter for eliminating therapeutic ultrasound noise with high-speed ultrasonic imaging in ultrasound-guided high-intensity focused ultrasound treatment (2016)

Abstract In the conventional ultrasonic monitoring of high-intensity focused ultrasound (HIFU) treatment, a significant interval between HIFU shots is required when monitoring target tissue to avoid interference between HIFU noise and RF echo signals. In our previous study, a new filtering method to eliminate only HIFU noise while maintaining tissue signals intact was proposed, and it was shown that the thermal coagulation could be detected during simultaneous HIFU irradiation through off-line processing. In this study, the filtering method and a real-time coagulation detection algorithm were implemented in an ultrasound imaging system, whose use for sequential exposure with multiple foci was demonstrated similarly to a commercial HIFU ablation system. The coagulation was automatically detected by the proposed method during real-time simultaneous HIFU irradiation, and the HIFU exposure time was controlled according to the changes in the tissue. The results imply that ultrasonic monitoring with the filtering and detection methods is useful for true real-time detection of changes in the tissue due to thermal coagulation during HIFU exposure.
Lysibodies are IgG Fc fusions with lysin binding domains targetingStaphylococcus aureuswall carbohydrates for effective phagocytosis (2017)

The cell wall of Gram-positive bacteria contains abundant surface-exposed carbohydrate molecules that are highly conserved within and often across species. The potential therapeutic usefulness of high-affinity antibodies to cell wall carbohydrates is unquestioned, however obtaining such antibodies is challenging due to the poor overall immunogenicity of these bacterial targets. Autolysins and phage lysins are peptidoglycan hydrolases, enzymes that have evolved over a billion years to degrade bacterial cell wall. Such wall hydrolases are modular enzymes, composed of discrete domains for high-affinity binding to cell wall carbohydrates and cleavage activity. In this study, we demonstrate that binding domains from autolysins and lysins can be fused to the Fc region of human IgG, creating a fully functional homodimer (or "lysibody") with high-affinity binding and specificity for carbohydrate determinants on the bacterial surface. Furthermore, we demonstrate that this process is reproducible with three different binding domains specific to methicillin-resistant <i>Staphylococcus aureus</i> (MRSA). Cell-bound lysibodies induced the fixation of complement on the bacterial surface, promoted phagocytosis by macrophages and neutrophils, and protected mice from MRSA infection in two model systems. The lysibody approach could be used to target a range of difficult-to-treat pathogenic bacteria, given that cell wall hydrolases are ubiquitous in nature.
Right ventricle-sparing left ventricular resection and replacement with a continuous-flow rotary blood pump: an in vivo experiment. (2010)

Despite recent advances in left ventricular assist device and total artificial heart technologies, these devices are still so large that they pose a significant problem in small patients with refractory heart failure. Excising the left ventricle while preserving the right ventricle--and then replacing the left ventricle with a mechanical pump--has been proposed as an alternative approach to this problem. We conducted a pilot study to evaluate possible surgical techniques and the hemodynamic effects of right ventricle-sparing left ventricular resection and replacement with a continuous-flow rotary blood pump in a healthy bovine model.
Measurement and analysis of the two-peak resonance of fish swimbladders (1993)

The object of this research is to study the frequency response of the two-chambered goldfish swimbladder. An ultrasonic system was devised to measure swimbladder frequency response invivo in a noninvasive, nonintrusive manner. Measurements were also made for oscars which have a single-chambered swimbladder. Unlike the oscar, two peaks often appear in the response curves of goldfish. To explain this two-peak resonance, an experimental model and an analytical model have been developed. The experimental model employs two gas bubbles to simulate the swimbladder. The experimental results show that the interaction between the two chambers of the goldfish swimbladder causes the two-peak phenomenon. The analytical model treats an oscillating system with two degrees of freedom. The parameters for the analytical model are related to the physical parameters of the system by theory. From comparison of the experimental and theoretical models, it is found that the damped-spring-mass system with two degrees of freedom reproduces what has been observed in the two-bubble experiment and does a reasonable job of modeling the two-chambered goldfish swimbladder.
Priority-Setting for Novel Drug Regimens to Treat Tuberculosis: An Epidemiologic Model (2017)

In designing clinical trials of novel TB regimens, investigators should consider that even small changes in treatment efficacy may have considerable impact on TB-related incidence and mortality. Other regimen improvements may still have important benefits for resource allocation and outcomes such as patient quality of life.
Animal models in systemic sclerosis. (2009)

Systemic sclerosis (SSc) is a complex systemic disease. It is characterised by fibrosis, of the skin and internal organs, as lungs, kidney, heart and gastrointestinal tract. As the aetiology of SSc is unknown, numerous animal models have been developed to better understand the pathogenesis of the disease and to test potentially useful therapeutic interventions. Although several animal models have been described, predominantly in mice, none reproduces precisely all manifestations of SSc. However, all animal models display tissue fibrotic changes similar to those present in SSc. This review is focused on the principal animal models and the molecular pathways involved. Animal models can be divided into two groups. In one group the pathologic phenotype is the result of a genetic mutation ( tight skin 1 and tight skin 2,UCD 200). In the second group, the pathologic alterations are induced in normal animals by manipulation of their immune system (sclerodermatous graft-vs-host disease (Scl GVHD) induced by transplantation, or by administration of exogenous substances). In the future, the development of additional animal models may become important in the further understanding of the alteration of the molecular pathways that regulate a physiologic processes to induce tissue fibrosis, the hallmark of this disease, and to identify and test targeted therapeutic agents.
Downregulation of galactomannan galactosyltransferase in Cyamopsis tetragonoloba (2023)

Abstract Guar is a legume of industrial importance due to the presence of gum (galactomannan) in its seed endosperm. The mannose (M)/galactose (G) ratio in galactomannan molecule is critical in deciding the physico-chemical properties of guar gum. Gum with reduced galactose content has better commercial value due to better viscosity. We report down regulation of galactomannan galactosyltransferase ( GMGT ) which links G residues to the M backbone in galactomannan molecule. The target GMGT sequence for RNAi was directionally cloned into pENTR TM /D-TOPO® vector, followed by a LR recombination reaction with pANDA vector. The constructed gmgt RNAi cassette was delivered into guar tissue using Agrobacterium tumefaciens -mediated gene transfer. The cotyledonary node explants were pre-cultured for 72 h and co-cultivated with A. tumefaciens strain harbouring the gmgt RNAi cassette for 24 h. Maximum number of putative transformed shoots regenerated from the cotyledonary node explants cultured in vitro on Murashige and Skoog (MS) medium containing 1.5 mg/L indole-3-acetic acid (IAA), 4.0 mg/L butyric acid (BA) and 1.0 mg/L gibberellic acid (GA 3 ). In vitro regenerated shoots were rooted on half-strength MS medium containing 4% sucrose and 2 mg/L indole-3-butyric acid (IBA). The quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) analysis showed reduced levels of GMGT expression in the seed endosperm of 35 days after flowering (DAF) stage pods. The present findings are expected to be beneficial in taking up of gene characterization and improvement of agronomic traits in guar.
High-brightness fiber coupled pumps (2009)

Advanced high volume applications require pumps with high power, high brightness, and high power efficiency. New generation devices meet all of these challenging requirements, while still maintaining the advantages of distributed pumping architecture including high reliability inherent to single emitter sources. Based on new-generation long-cavity diode chips, new pumps are capable of more than 60W CW power ex-fiber output (100 &mu;m core diameter) into NA ~ 0.12. Peak power efficiency stays over 60%. All of the above is provided at room heatsink temperature, maintained by basic air- or water-cooling.
<title>Design of a variable twist tilt-rotor blade using shape memory alloy (SMA) actuators</title> (2001)

This paper presents research aimed at actively altering the twist distribution of a tiltrotor blade between hover and forward flight. Three different concepts-extension-twist coupled composites, bimoment actuation and discrete SMA torque tube actuation - are considered, and the torque tube appears the most feasible. Parametric design of the torque tube and attachment technique is presented with actuation torque, heat transfer and bandwidth issues being considered to arrive at the configuration of the tube. The effect of heat treatment of the SMA in tuning the actuation characteristics is discussed. A dramatic improvement in the actuation cooling time is demonstrated through the use of active cooling using thermodelectric modules. An extension of the one-dimensional formulation of Brinson's model to the torsional case is presented. The model is shown to have good correlation with room temperature characteristics. The criterion for impedance matching between the actuator and the host structure is derived. The torsional actuator is tested both under no load and acting against a restoring spring and shows repeatable actuation characteristics.
Perfluoroalkylation of the 2-nitropropyl anion. Evidence for an SRN1 process (1983)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPerfluoroalkylation of the 2-nitropropyl anion. Evidence for an SRN1 processAndrew E. FeiringCite this: J. Org. Chem. 1983, 48, 3, 347–354Publication Date (Print):February 1, 1983Publication History Published online1 May 2002Published inissue 1 February 1983https://pubs.acs.org/doi/10.1021/jo00151a014https://doi.org/10.1021/jo00151a014research-articleACS PublicationsRequest reuse permissionsArticle Views380Altmetric-Citations61LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Partial discharge characteristics in layered barium titanate structures (1984)

Pd characteristics for MLC capacitors with different relative permittivities, porosity, and void size distributions were evaluated. The results show that: 1. Pd is a strong function of and increases with the increase of the applied field. 2. Higher dielectric constant, which gives a bigger field enhancement, also enhances the Pd activity. 3. The source of Pd is the ionization discharge across the voids in the bulk as well as those adjacent to the electrodes. Knowing the void size distribution, the Pd characteristics are generally predictable. 4. The cause of low breakdown for a dielectric with high porosity is that the regions of thin dielectric associated with porosity cannot withstand a high field as the voltage across a void drops significantly by the partial discharge.
Protective host defense against disseminated candidiasis is impaired in mice expressing human interleukin-37 (2015)

The effect of the anti-inflammatory cytokine interleukin-37 (IL-37) on host defense against Candida infections remains unknown. We assessed the role of IL-37 in a murine model of disseminated candidiasis using mice transgenic for human IL-37 (hIL-37Tg). Upon exposure to Candida albicans pseudohyphae, macrophages from hIL-37Tg mice release 39% less TNFα compared to cells from wild-type (WT) mice (p = 0.01). In vivo, hIL-37Tg mice displayed a decreased capacity to recruit neutrophils to the site of infection. These defects were associated with increased mortality and organ fungal growth in hIL-37Tg compared to WT mice. We conclude that IL-37 interferes with the innate protective anti-Candida host response by reducing the production of proinflammatory cytokines and suppressing neutrophil recruitment in response to Candida, resulting in an increased susceptibility to disseminated candidiasis.
Evaluation of body fluid samples with X-ray phase-contrast imaging (2015)

Poster: ECR 2015 / C-0667 / Evaluation of body fluid samples with X-ray phase-contrast imaging by: V. Toth 1, A. A. Fingerle1, J. Henningsen2, L. Birnbacher2, M. S. Willner2, J. Herzen2, P. B. Noel1, E. J. Rummeny1, F. Pfeiffer2; 1Munich/DE, 2Garching/DE
Supplementary Methods from Identification of Epigenetically Silenced Genes in Tumor Endothelial Cells (2023)

Supplementary Methods from Identification of Epigenetically Silenced Genes in Tumor Endothelial Cells
Description of a Flow Optimized Oxygenator With Integrated Pulsatile Pump (2010)

Extracorporeal membrane oxygenation (ECMO) is a well-established therapy for several lung and heart diseases in the field of neonatal and pediatric medicine (e.g., acute respiratory distress syndrome, congenital heart failure, cardiomyopathy). Current ECMO systems are typically composed of an oxygenator and a separate nonpulsatile blood pump. An oxygenator with an integrated pulsatile blood pump for small infant ECMO was developed, and this novel concept was tested regarding functionality and gas exchange rate. Pulsating silicone tubes (STs) were driven by air pressure and placed inside the cylindrical fiber bundle of an oxygenator to be used as a pump module. The findings of this study confirm that pumping blood with STs is a viable option for the future. The maximum gas exchange rate for oxygen is 48mL/min/L(blood) at a medium blood flow rate of about 300mL/min. Future design steps were identified to optimize the flow field through the fiber bundle to achieve a higher gas exchange rate. First, the packing density of the hollow-fiber bundle was lower than commercial oxygenators due to the manual manufacturing. By increasing this packing density, the gas exchange rate would increase accordingly. Second, distribution plates for a more uniform blood flow can be placed at the inlet and outlet of the oxygenator. Third, the hollow-fiber membranes can be individually placed to ensure equal distances between the surrounding hollow fibers.
400.6-Gb/s PDM-BPSK Superchannel Transmission over 12,800-km TWRS Fiber via Nonlinear Noise Squeezing (2013)

We transmit a 406.6-Gb/s superchannel consisting of eight 37.5-GHz-spaced 32-Gbaud Nyquist-filtered PDM-BPSK signals over 12,800-km (160×80-km) EDFA-only amplified dispersion-unmanaged TWRS fiber. This result is enabled by nonlinear noise squeezing achieved through optimized digital electronic dispersion pre-compensation.
Dynamic scheduling in make-to-stock production systems with setup times and random breakdowns: performance analysis and improved policies (2021)

We study the scheduling of production in a multi-item, failure-prone machine with setup times, with the objective of minimising long-run average inventory and backlog costs. We make two contributions to this problem, which has received significantly less attention compared to the standard stochastic economic lot scheduling problem (with random demand and production times). First, we adapt and implement a variety of existing scheduling policies and compare their performance through a set of numerical experiments spanning a wide range of operating conditions. This analysis allows us to identify important shortcomings of the policies, including their poor performance when items have widely different priorities (as dictated by the product of their cost and production rate parameters). Second, we leverage these insights to develop and analyse a new, easy-to-implement policy. This policy tightly controls the surplus of the highest-priority items using fixed base-stock levels and, for all other items, it determines their run lengths dynamically in such a way that long production runs become decreasingly likely with decreasing item priority. Our experiments show that our new policy greatly outperforms the benchmarking policies over a large and important set of operating regimes, comprising high-utilisation systems where the machine cycles through all items at a frequency comparable to or greater than the frequency of failure events.
Exploiting recent trends for the synthesis and surface functionalization of mesoporous silica nanoparticles towards biomedical applications (2022)

Rapid progress in developing multifunctional nanocarriers for drug delivery has been observed in recent years. Inorganic mesoporous silica nanocarriers (MSNs), emerged as an ideal candidate for gene/drug delivery with distinctive morphological features. These ordered carriers of porous nature have gained unique attention due to their distinctive features. Moreover, transformation can be made to these nanocarriers in terms of pores size, pores volume, and particle size by altering specific parameters during synthesis. These ordered porous materials have earned special attention as a drug carrier for treating multiple diseases. Herein, we highlight the strategies employed in synthesizing and functionalizing these versatile nanocarriers. In addition, the various factors that influence their sizes and morphological features were also discussed. The article also summarizes the recent advancements and strategies for drug and gene delivery by rendering smarter MSNs by incorporating functional groups on their surfaces. Averting off-target effects through various capping strategies is a massive milestone for the induction of stimuli-responsive nanocarriers that brings out a great revolution in the biomedical field.
dIVI-pd: Dual-Stateless IPv4/IPv6 Translation with Prefix Delegation (2011)

This document presents the address specifications and deployment
considerations of address-sharing dual stateless IPv4/IPv6 translation
with prefix delegation (dIVI-pd). The dIVI-pd keeps the features of
stateless, end-to-end address transparency and bidirectional-initiated
communications of the original stateless IPv4/IPv6 translation, while
it can utilize the IPv4 addresses more effectively. In addition, it
does not require the DNS64 and ALG, and can be used with prefix
delegation.
The Use of Computerized Tomography Scanning to Localize the Impalpable Testis (1983)

To determine the usefulness of computerized tomography scanning in the preoperative localization of the impalpable undescended testis 5 patients with 8 impalpable testes were studied. In 2 patients the scan correctly outlined 3 impalpable testes inside the internal inguinal ring, in 2 patients the scan correctly localized 4 impalpable testes at or just inside the external inguinal ring and in 1 patient the scan failed to demonstrate a testis, a finding corroborated at an operation. These results suggest that computerized tomography scanning appears to be an effective, simple and less hazardous method to localize impalpable undescended testes than other currently available techniques.