gebhart/patrepeval huggingface dataset:
Identification of a novel selD homolog from Eukaryotes, Bacteria, and Archaea: Is there an autoregulatory mechanism in selenocysteine metabolism? (1996)

Escherichia coli selenophosphate synthetase (SPS, the selD gene product) catalyzes the production of monoselenophosphate, the selenium donor compound required for synthesis of selenocysteine (Sec) and seleno-tRNAs. We report the molecular cloning of human and mouse homologs of the selD gene, designated Sps2 , which contains an in-frame TGA codon at a site corresponding to the enzyme’s putative active site. These sequences allow the identification of selD gene homologs in the genomes of the bacterium Haemophilus influenzae and the archaeon Methanococcus jannaschii , which had been previously misinterpreted due to their in-frame TGA codon. Sps2 mRNA levels are elevated in organs previously implicated in the synthesis of selenoproteins and in active sites of blood cell development. In addition, we show that Sps2 mRNA is up-regulated upon activation of T lymphocytes and have mapped the Sps2 gene to mouse chromosome 7. Using the mouse gene isolated from the hematopoietic cell line FDCPmixA4, we devised a construct for protein expression that results in the insertion of a FLAG tag sequence at the N terminus of the SPS2 protein. This strategy allowed us to document the readthrough of the in-frame TGA codon and the incorporation of 75 Se into SPS2. These results suggest the existence of an autoregulatory mechanism involving the incorporation of Sec into SPS2 that might be relevant to blood cell biology. This mechanism is likely to have been present in ancient life forms and conserved in a variety of living organisms from all domains of life.
Nanosphere Lithography:  Fabrication of Large-Area Ag Nanoparticle Arrays by Convective Self-Assembly and Their Characterization by Scanning UV−Visible Extinction Spectroscopy (2004)

This work employs UV-visible extinction spectroscopy as a new spectral mapping technique to characterize self-assembled polystyrene microsphere samples produced by convective self-assembly (CSA). This spectroscopic technique was successfully used to analyze the periodic particle arrays produced by the polystyrene template, yielding a detailed characterization of each sample. The CSA-prepared samples proved to be more uniform across a sample as well as more reproducible than previous sample preparation techniques. For the first time, a detailed characterization and quantitative evaluation of the entire sample has been performed by spectroscopic mapping.
The case for a hybrid passive/active network monitoring scheme in the wireless Internet (2002)

This paper discusses monitoring within wired and wireless networks and the type of monitoring information needed to support different applications. We suggest a hybrid active/passive monitoring approach with a dynamic time window mechanism and interchangeable filters to extract requested information. The paper also shows our initial experimental results and presents our conclusions.
Tumor Microenvironment Remodeling Enables Bypass of Oncogenic KRAS Dependency in Pancreatic Cancer (2020)

Oncogenic KRAS (KRAS*) is a key tumor maintenance gene in pancreatic ductal adenocarcinoma (PDAC), motivating pharmacologic targeting of KRAS* and its effectors. Here, we explored mechanisms involving the tumor microenvironment (TME) as a potential basis for resistance to targeting KRAS*. Using the inducible <i>Kras</i> <sup>G12D</sup>;<i>Trp53</i> <sup>-/-</sup> PDAC mouse model, gain-of-function screens of epigenetic regulators identified <i>HDAC5</i> as the top hit enabling KRAS* independent tumor growth. <i>HDAC5</i>-driven escaper tumors showed a prominent neutrophil-to-macrophage switch relative to KRAS*-driven tumors. Mechanistically, HDAC5 represses <i>Socs3</i>, a negative regulator of chemokine CCL2, resulting in increased CCL2, which recruits CCR2<sup>+</sup> macrophages. Correspondingly, enforced <i>Ccl2</i> promotes macrophage recruitment into the TME and enables tumor recurrence following KRAS* extinction. These tumor-associated macrophages in turn provide cancer cells with trophic support including TGFβ to enable KRAS* bypass in a SMAD4-dependent manner. Our work uncovers a KRAS* resistance mechanism involving immune cell remodeling of the PDAC TME. SIGNIFICANCE: Although KRAS* is required for PDAC tumor maintenance, tumors can recur following KRAS* extinction. The capacity of PDAC cancer cells to alter the TME myeloid cell composition to support KRAS*-independent tumor growth illuminates novel therapeutic targets that may enhance the effectiveness of therapies targeting KRAS* and its pathway components.<i>See related commentary by Carr and Fernandez-Zapico, p. 910</i>.<i>This article is highlighted in the In This Issue feature, p. 890</i>.
Cryptococcus neoformansin Organ Transplant Recipients: Impact of Calcineurin‐Inhibitor Agents on Mortality (2007)

Variables influencing the risk of dissemination and outcome of Cryptococcus neoformans infection were assessed in 111 organ transplant recipients with cryptococcosis in a prospective, multicenter, international study. Sixty-one percent (68/111) of the patients had disseminated infection. The risk of disseminated cryptococcosis was significantly higher for liver transplant recipients (adjusted hazard ratio [HR], 6.65; P=.048). The overall mortality rate at 90 days was 14% (16/111). The mortality rate was higher in patients with abnormal mental status (P=.023), renal failure at baseline (P=.028), fungemia (P=.006), and disseminated infection (P=.035) and was lower in those receiving a calcineurin-inhibitor agent (P=.003). In a multivariable analysis, the receipt of a calcineurin-inhibitor agent was independently associated with a lower mortality (adjusted HR, 0.21; P=.008), and renal failure at baseline with a higher mortality rate (adjusted HR, 3.14; P=.037). Thus, outcome in transplant recipients with cryptococcosis appears to be influenced by the type of immunosuppressive agent employed. Additionally, discerning the basis for transplant type-specific differences in disease severity has implications relevant for yielding further insights into the pathogenesis of C. neoformans infection in transplant recipients.
Unanticipated results in the first direct measurements of anchor damping in MEMS resonators (2018)

In this study, a novel method of measuring the Quality Factor (Q) of a MEMS tuning fork resonator at low temperature enabled us to accurately pinpoint the energy dissipation resulting from anchor damping [1]. We used this new method to perform the first direct examination of the impact of design of the anchors and the die mounting structures on the strength of anchor damping. Our results show that the elastic energy can escape the resonator through the anchor(s), and still be retained within the die, and that energy stored in the die can return to the resonator. Thus, the measured Q of the resonator is increased, depending on mounting structures far from the resonating element. Moreover, our data shows that the number of anchors does not change the total energy dissipation through the anchors.
Proteomic Analysis of the Luteal Endometrial Secretome (2020)

Endometrium attains a secretory architecture in preparation for embryo implantation, but the identity of most endometrial secretory products remains unknown. Our objective was to characterize the endometrial secretome and compare protein expression between prereceptive (luteinizing hormone [LH]+4) receptive (LH+9) and phase endometrium. Endometrial lavage was performed in 11 participants and analyzed by difference gel electrophoresis (DIGE). LH+4 and LH+9 specimens were labeled with cyanine fluorescent dyes Cy3 and Cy5 tags, respectively, and combined. Proteins were separated using 2-dimensional gel electrophoresis, isolated, trypsin-digested, and subjected to mass spectrometry. In all, 152 proteins were identified; 82 were differentially expressed. Most proteins with increased expression on LH+9 functioned in host defense, while proteins with decreased expression had many functions. A total of 14 proteins had changes suggesting altered posttranslational modification. This article describes the first application of proteomic analysis to endometrial secretions, allowing identification of novel endometrial proteins as well as those differentially secreted in prereceptive and receptive phases.
Complex rotating vector method for smooth torque control of a saturated switched reluctance motor (2003)

This paper describes novel complex rotating vector methods for calculating the air gap torque of a switched reluctance motor (SRM). These vector methods are used to derive a number of insightful methods for improving the smoothness of the SRM's air gap torque. The development covers both unsaturated as well as saturated SRMs. Theoretical methods are developed, alternative implementations of such rotating vector methods are described and experimental results are included on a commercial, unsaturated SRM and on a commercial, saturated SRM with asymmetry. The results verify the capability of the proposed vector methods.
Contextual vector quantization for speech recognition with discrete hidden Markov model (2002)

By using formulation of the finite mixture distribution identification, several alternatives to the conventional LBG VQ method are investigated. A contextual VQ method based on the Markov random field (MRF) theory is proposed to model the speech feature vector space. Its superiority is confirmed by a series of comparative experiments in a speaker independent isolated word recognition task by using different VQ schemes as the front-end of DHMM. The VQ schemes studied include the LBG VQ, the classification maximum likelihood (CML) approach, the mixture maximum likelihood (MML) procedure, the ergodic large HMM (LHMM) and the contextual VQ (CVQ) method. The motivation to use the MRF to model the contextual dependence information in the underlying speech production process can be readily extended to acoustic modeling of the basic speech units in speech recognition.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
The Shrink-Wrapped VPN Node (2000)

The wide availability of public domain IPsec implementations allows the creation of VPNs based on low-cost platforms. However, setting up a VPN node involves a lot of work such as the creation of IPsec Security Associations and associated tunnels, including the necessary management of keys. Moreover, routing and firewall facilities must be provided to ensure the isolation of the members of the VPN from the public Internet. In this paper we present a drop-in VPN node that is compact, low-cost and requires little administration or maintenance. We discuss the features and advantages of our system. Next, we demonstrate how this system was used for the creation of a VPN linking networks within the University campus with others located in outside locations (e.g. other companies, home networks etc.) Finally, we present our evaluation of the work performed and describe our future plans.
Control Oriented Modeling and Nonlinear Model Predictive Control of Advanced SI Engine System (2010)

Control oriented model (COM) using crank-angle resolved flame propagation simulation and nonlinear model predictive control (NMPC) methodology for the purpose of transient control of HDOF engines are proposed in this paper. The nonlinear nature of the combustion process has been a challenge in building a reliable COM and engine simulation. Artificial neural networks (ANNs) are subsequently trained on the data generated with a quasi-D combustion model to create fast surrogate combustion models. System dynamics are augmented by manifold and actuator dynamics models. Then, NMPC for an internal combustion (IC) engine with a dual-independent variable valve timing (VVT) system is designed to achieve fast torque responses, to eliminate exhaust emissions penalty, and to track the optimal actuator response closely. The NMPC significantly improves engine dynamics and minimizes excursions of in-cylinder variables under highly transient operation. Dead-beat like control is achieved with selected prediction horizon and control horizon in the NMPC.
BLUE FLUORESCENT HOST- RED PHOSPHORESCENT GUEST BEARING POLYNORBORNENE (2007)

For applications often a host material is necessary, which has to be selected carefully, e.g., in terms of providing efficient Forster energy transfer to name one important prerequisite. Polymeric hosts are of great interest as they are more amenable to solution processing techniques such as spin-coating or inkjet printing than small molecules. Moreover, covalent incorporation of the guest into the host-polymer constitutes a further step forward for an easy and reliable processing of the material. Herein we wish to report our endeavors to prepare polymerizable platinum(II) complexes by discussion of the synthesis and photophysical properties of a Pt(II)quinolinolate derivative statistically copolymerized with a suitable luminescent host material. As the polymerization method Ring Opening Metathesis Polymerization (ROMP) was used because of its excellent functional group tolerance. 4 As revealed by photophysical measurements, efficient energy transfer from the host to the platinum-guest in the solid state occurred. As a result, red emission of the platinum complex could be obtained by exciting the host material at 360 nm. Experimental Materials. Unless otherwise noted, materials were obtained from commercial sources (Aldrich, Fluka or Lancaster) and were used without further purification. 5-Formyl-8-hydroxyquinoline 5 and the initiator (H2IMes)(pyridine)2RuCl2(=CHPh) (G) 6a
Catching and Releasing of Small Parts Using Capillary Forces (2004)

info:eu-repo/semantics/published
Advanced diode-pumped solid state laseres (DPSSLs): near-term trends and future prospects (2000)

The features of modern, high-power semiconductor diode laser arrays as sources for pumping high power solid state lasers are reviewed. The status and prospects for high power, high- beam quality Nd:YAG and Yb:YAG DPSSLs are examined. Developing concepts for novel high power DPSSLs are also outlined.
Twitsper: Tweeting privately (2013)

Although today's online social networks provide some privacy controls to protect a user's shared content from other users, these controls aren't sufficiently expressive to provide fine-grained protection. Twitsper offers fine-grained control over who sees a Twitter user's messages, enabling private group communication while preserving Twitter's commercial interests.
Supplementary Table 1 - 5 from <i>PARD3</i> Inactivation in Lung Squamous Cell Carcinomas Impairs STAT3 and Promotes Malignant Invasion (2023)

&lt;p&gt;Table S1. List of lung cancer cell lines and histopathological type used to determine the status of the PARD3. Table S2. List of primers used for cloning, PCR and Sanger sequencing of PARD3. Table S3. List of single nucleotide polymorphisms (SNPs) and variants of unknown biological significance identified in lung cancer cell lines and lung primary tumors tested. Table S4. Clinical and pathological parameters of the NSCLC patients and tumors included in the PARD3 gene alterations screening. Table S5. List of genes selected from those genes that fulfilled the criteria: i) adjusted p&lt;0.05; ii) at least 1.5-fold induction or repression in the wild type- H157tr-wtPAR3 (dox+) relative to H157tr (dox+).&lt;/p&gt;
Comparing human middle-ear motion and pressure gain across idealized and progressively more realistic three-dimensional finite-element models (2015)

The mammalian tympanic membrane (TM) is linked to the cochlea via a flexible and circuitous three-bone ossicular chain for which much of the mass is concentrated away from the entry axis to the cochlea. As the TM area (ATM) is much larger than that of the stapes footplate (AFP) and the length of the malleus (LM) is somewhat longer than the incus (LI), the middle ear is considered to function as a pressure transformer to optimize the flow of vibrations from low-density air to high-density cochlear fluid, with an ideal pressure gain defined as (ATM/AFP)*(LM/LI). Even so, the reasons for this complex ossicular arrangement, as opposed to the more straightforward case of a columella directly connecting the TM to the cochlea, are not entirely clear. We explore the effects of middle-ear anatomy and material properties on ossicular motion and pressure gain by comparing the behavior of a series of 3D finite-element models ranging from idealized simple axisymmetric cases of a flat-circular or conic TM connected to a columella, all the way to an anatomically accurate three-ossicle human middle-ear model based on a micro-CT scan of a temporal bone. In doing this, we will test whether the complex 3D anatomy of the human middle ear can be shown to offer any concrete advantages in terms of pressure gain over a simpler columella design, or whether other possible reasons for this complex design are more likely. [Work supported by R01 DC05960 from the NIDCD of the NIH.]
InP DHBT 68 GHz frequency divider (2003)

We report a 68 GHz 2:1 frequency divider with measured input sensitivity less than 6 dBm from 53-68 GHz. To our knowledge, this is the fastest digital frequency divider reported to date in any semiconductor technology. The divider is implemented in differential current mode logic (CML) using undercut collector InP Double Heterojunction Bipolar Transistor (DHBT) technology, with measured f/sub T/ of 150 GHz and f/sub max/ of 163 GHz at J/sub x/=1.2/spl times/10/sup 5/ A/cm/sup 2/. It draws 25.1 mA from a single -3 V supply, dissipating 75 mW. Test results were obtained for wafer probe using micro-coax probes.
Postprocessing Reduces Pulsation Artifacts and Increases Visibility of Liver Lesions in Flow-compensated Diffusion-weighted Imaging (2023)

Diffusion-weighted imaging of the liver is prone to the cardiac pulsation artifact, which can lead to reduced lesion visibility. We addressed this problem with a two-fold approach. First, flow-compensated diffusion weightings were used, which are known to reduce this artifact. Using a dataset of 40 patients suffering from focal liver lesions, we addressed the remaining signal voids with different postprocessing techniques, namely weighted averaging, the p-mean approach, and an outlier exclusion algorithm. The algorithms substantially increased the lesion visibility and further reduced the pulsation artifact. An evaluation of CNR and calculation time showed that weighted averaging was suited best.
Nonperturbative analysis of the evolution of cosmological perturbations through a nonsingular bounce (2013)

In bouncing cosmology, the primordial fluctuations are generated in a cosmic contraction phase before the bounce into the current expansion phase. For a nonsingular bounce, curvature and anisotropy grow rapidly during the bouncing phase, raising questions about the reliability of perturbative analysis. In this paper, we study the evolution of adiabatic perturbations in a nonsingular bounce by nonperturbative methods including numerical simulations of the nonsingular bounce and the covariant formalism for calculating nonlinear perturbations. We show that the bounce is disrupted in regions of the universe with significant inhomogeneity and anisotropy over the background energy density but is achieved in regions that are relatively homogeneous and isotropic. Sufficiently small perturbations, consistent with observational constraints, can pass through the nonsingular bounce with negligible alteration from nonlinearity. We follow scale invariant perturbations generated in a matterlike contraction phase through the bounce. Their amplitude in the expansion phase is determined by the growing mode in the contraction phase, and the scale invariance is well preserved across the bounce.
Nuevo Modelo Polinomial del Comportamiento de un Cilindro Neumático (2006)

En este articulo se muestra un nuevo modelo matematico asociado al comportamiento de un cilindro neumatico con amortiguamiento en ambos lados, el cual es desarrollado mediante ecuaciones polinomicas en sustitucion de las ecuaciones termomecanicas originales. Se utiliza el criterio del coeficiente de correlacion para validar el grado de la aproximacion del nuevo modelo desarrollado. En este nuevo modelo se comprueba que el tiempo de simulacion es menor que en el caso del modelo original, agilizando de esta forma la prediccion del comportamiento de este tipo de actuador
Correction: Magnetic control of cellular processes using biofunctional nanoparticles (2017)

[This corrects the article DOI: 10.1039/C7SC01462G.].
Holographic Servo-signal Detection Stabilized to the Wavelength Fluctuation and the Mechanical Misalignment in the Optical Heads (1989)

Among recent studies on holographic optical elements(HOEs) are those to develop compact substitutes for the beamsplitting prism and lens systems in the optical heads. The holographic heads 1-4 presented so far, however, have been based on the conventional servo-detection methods that required precise beam-positioning at the photodetectors. Thus HOEs, applied to both the astigmatic optics 2,3 and the double knife-edge method, 1,3,4 suffered some serious drawback due to the wavelength shift of the diode laser source, typically Δλ ≳ 5nm. 1,2
Supplementary figures S1-5, Tables S1-5 from XPO1/CRM1 Inhibition Causes Antitumor Effects by Mitochondrial Accumulation of eIF5A (2023)

&lt;p&gt;Supplementary Figures S1-5, Tables S1-5. Figure S1 The Cytotoxic Effect of KPT-185 is Tumor Suppressor-independent. Figure S2 Combination Index of KPT-185 with Chemotherapeutic Agents. Figure S3 1D Gel Analysis of Cytoplasmic Protein. Figure S4 Effects of Selinexor (KPT-330) in an Ovarian Cancer Mouse Model. Figure S5 The Efficacy of Selinexor (KPT-330) on Tumor Regression and Metastasis Table S1 p53 status and IC50 concentration of KPT-185 after 72 hours of incubation in human cancer cell lines Table S2 Combination index (CI) of KPT-185 with cytotoxic agents Table S3 Proteins showing altered mitochondrial localization after treatment with KPT-185 Table S4 1D Proteomic analysis of cytoplasmic proteins immunoprecipitated with eIF5A Table S5 siRNA Sequences&lt;/p&gt;
Supported Metallocene Catalysis for In Situ Synthesis of High Energy Density Metal Oxide Nanocomposites (2007)

The 0−3 metal oxide−isotactic polypropylene nanocomposites are synthesized via in situ propylene polymerization using the C2-symmetric metallocene catalyst dichloro[rac-ethylenebisindenyl]zirconium(IV) (EBIZrCl2) immobilized on methylaluminoxane (MAO)-treated barium titanate (BaTiO3) or titanium dioxide (TiO2) nanoparticles. The composite materials are characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and 13C nuclear magnetic resonance (NMR) spectroscopy. It is shown that the nanoparticles are homogeneously dispersed in the polyolefin matrices. Electrical measurements reveal nanocomposite leakage current densities of ∼10-6 to 10-9 A/cm2, permittivities as high as 6.1, and breakdown strengths of ∼4 MV/cm. Energy densities are estimated to be as high as 9.4 J/cm3.