gebhart/patrepeval huggingface dataset:
Label‐Free Imaging of Membrane Potentials by Intramembrane Field Modulation, Assessed by Second Harmonic Generation Microscopy (2022)

Optical interrogation of cellular electrical activity has proven itself essential for understanding cellular function and communication in complex networks. Voltage-sensitive dyes are important tools for assessing excitability but these highly lipophilic sensors may affect cellular function. Label-free techniques offer a major advantage as they eliminate the need for these external probes. In this work, it is shown that endogenous second-harmonic generation (SHG) from live cells is highly sensitive to changes in transmembrane potential (TMP). Simultaneous electrophysiological control of a living human embryonic kidney (HEK293T) cell, through a whole-cell voltage-clamp reveals a linear relation between the SHG intensity and membrane voltage. The results suggest that due to the high ionic strengths and fast optical response of biofluids, membrane hydration is not the main contributor to the observed field sensitivity. A conceptual framework is further provided that indicates that the SHG voltage sensitivity reflects the electric field within the biological asymmetric lipid bilayer owing to a nonzero <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:msubsup><mml:mi>χ</mml:mi><mml:mtext>eff</mml:mtext><mml:mrow><mml:mo>(</mml:mo><mml:mn>2</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:msubsup></mml:mrow></mml:math> tensor. Changing the TMP without surface modifications such as electrolyte screening offers high optical sensitivity to membrane voltage (≈40% per 100 mV), indicating the power of SHG for label-free read-out. These results hold promise for the design of a non-invasive label-free read-out tool for electrogenic cells.
Chortle: a technology mapping program for lookup table-based field programmable gate arrays (2002)

An algorithm is described for technology mapping of combinational logic into field programmable gate arrays that use lookup table memories to realize combinational functions. It is difficult to map into lookup tables using previous techniques because a single lookup table can perform a large number of logic functions and prior approaches require each function to be instantiated separately in a library. The new algorithm, implemented in a program called Chortle, uses the fact that a K-input lookup table can implement any Boolean function of K inputs and so does not require a library-based approach. Chortle takes advantage of this complete functionality to evaluate all possible decompositions of the input Boolean network nodes. It can determine the optimal (in area) mapping for fanout-free trees of combinational logic. In comparison with the MIS II technology mapper, on MCNC-89 LOGIC Synthesis benchmarks Chortle achieves superior results in significantly less time.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Multimodal Clustering Networks for Self-supervised Learning from Unlabeled Videos (2021)

Multimodal self-supervised learning is getting more and more attention as it allows not only to train large networks without human supervision but also to search and retrieve data across various modalities. In this context, this paper proposes a framework that, starting from a pre-trained backbone, learns a common multimodal embedding space that, in addition to sharing representations across different modalities, enforces a grouping of semantically similar instances. To this end, we extend the concept of instance-level contrastive learning with a multimodal clustering step in the training pipeline to capture semantic similarities across modalities. The resulting embedding space enables retrieval of samples across all modalities, even from unseen datasets and different domains. To evaluate our approach, we train our model on the HowTo100M dataset and evaluate its zero-shot retrieval capabilities in two challenging domains, namely text-to-video retrieval, and temporal action localization, showing state-of-the-art results on four different datasets.
Strategies for reducing the light shift in atomic clocks (2015)

Recent progress in optical lattice clocks requires unprecedented precision in controlling systematic uncertainties at the ${10}^{\ensuremath{-}18}$ level. Tuning of nonlinear light shifts is shown to reduce lattice-induced clock shift for a wide range of lattice intensity. Based on theoretical multipolar, nonlinear, anharmonic, and higher-order light shifts, we numerically demonstrate possible strategies for Sr, Yb, and Hg clocks to achieve lattice-induced systematic uncertainty below $1\ifmmode\times\else\texttimes\fi{}{10}^{\ensuremath{-}18}$.
Balloon aortic valvuloplasty in a 1,600‐gram infant (2000)

We present a 1,600 g infant who underwent successful balloon aortic valvuloplasty from the right carotid artery approach. A simple technique to facilitate access to the left ventricle and expedite the procedure is described. Issues unique to performing balloon aortic valvuloplasty on such a small child are discussed. Cathet. Cardiovasc. Intervent. 50:322–325, 2000. © 2000 Wiley-Liss, Inc.
Eine organische Verbindung mit Leiteranordnung der Spins (1997)

Paare aus organischen Donor(D)‐Stapeln bilden in dem Salz [(DT‐TTF) 2 ][Au(mmt) 2 ] (siehe Bild rechts) eine klassische Leiterstruktur mit drei S…S‐Kontaktwechselwirkungen. Unterhalb 225 K bilden die Doppelstapel aus Donormolekülen eine „zweibeinige Spinleiter”︁. die durch Lokalisation von ungepaarten Elektronen in den (DT‐TTF) 2 ,‐Dimeren entsteht. DT‐TTF = „Dithiophentetrathiafulvalen”︁, mnt = Maleinsäurenitrildithiolat. magnified image
A novel male Japanese quail structural connectivity atlas using ultra-high field diffusion MRI at 11.7 T (2022)

The structural connectivity of animal brains can be revealed using post-mortem diffusion-weighted magnetic resonance imaging (MRI). Despite the existence of several structural atlases of avian brains, few of them address the bird's structural connectivity. In this study, a novel atlas of the structural connectivity is proposed for the male Japanese quail (Coturnix japonica), aiming at investigating two lines divergent on their emotionality trait: the short tonic immobility (STI) and the long tonic immobility (LTI) lines. The STI line presents a low emotionality trait, while the LTI line expresses a high emotionality trait. 21 male Japanese quail brains from both lines were scanned post-mortem for this study, using a preclinical Bruker 11.7 T MRI scanner. Diffusion-weighted MRI was performed using a 3D segmented echo planar imaging (EPI) pulsed gradient spin-echo (PGSE) sequence with a 200 [Formula: see text]m isotropic resolution, 75 diffusion-encoding directions and a b-value fixed at 4500 s/mm<sup>2</sup>. Anatomical MRI was likewise performed using a 2D anatomical T<sub>2</sub>-weighted spin-echo (SE) sequence with a 150 [Formula: see text]m isotropic resolution. This very first anatomical connectivity atlas of the male Japanese quail reveals 34 labeled fiber tracts and the existence of structural differences between the connectivity patterns characterizing the two lines. Thus, the link between the male Japanese quail's connectivity and its underlying anatomical structures has reached a better understanding.
SI‐CLP inhibits the growth of mouse mammary adenocarcinoma by preventing recruitment of tumor‐associated macrophages (2019)

Chitinase-like proteins (CLP) are chitin-binding proteins that lack chitin hydrolyzing activity, but possess cytokine-like and growth factor-like properties, and play crucial role in intercellular crosstalk. Both human and mice express two members of CLP family: YKL-40 and stabilin-1 interacting chitinase-like protein (SI-CLP). Despite numerous reports indicating the role of YKL-40 in the support of angiogenesis, tumor cell proliferation, invasion and metastasis, the role of its structurally related protein SI-CLP in cancer was not reported. Using gain-of-function approach, we demonstrate in the current study that the expression of recombinant SI-CLP in mouse TS/A mammary adenocarcinoma cells results in significant and persistent inhibition of in vivo tumor growth. Using quantitative immunohistochemistry, we show that on the cellular level this phenomenon is associated with reduced infiltration of tumor-associated macrophages (TAMs), CD4+ and FoxP3+ cells in SI-CLP expressing tumors. Gene expression analysis in TAM isolated from SI-CLP-expressing and control tumors demonstrated that SI-CLP does not affect macrophage phenotype. However, SI-CLP significantly inhibited migration of murine bone-marrow derived macrophages and human primary monocytes toward monocyte-recruiting chemokine CCL2 produced in the tumor microenvironment (TME). Mechanistically, SI-CLP did not affect CCL2/CCR2 interaction, but suppressed cytoskeletal rearrangements in response to CCL2. Altogether, our data indicate that SI-CLP functions as a tumor growth inhibitor in mouse breast cancer by altering cellular composition of TME and blocking cytokine-induced TAM recruitment. Taking into consideration weak to absent expression of SI-CLP in human breast cancer, it can be considered as a therapeutic protein to block TAM-mediated support of breast tumor growth.
Visual Information Sensing Technology. Design and Implementation of Image Compression Sensor Based on Column Parallel Architecture. (1997)

We propose a novel image sensor which compresses image data on its sensor plane. The image compression sensor can significantly reduce the amount of pixel data output from the sensor. The proposed sensor is intended to overcome the communication bottleneck in high-pixel-rate imaging, such as high-frame-rate and high-resolution imaging. The compression algorithm is based on conditional replenishment. It detects motion and encodes only the pixels in moving areas. This paper describes the design and implementation of the proposed sensor based on column parallel architecture. In this architecture, fill factor and power dissipation are at a similar level to those of conventional MOS sensors. We show the results of some experiments using a prototype chip.
International Quality Systems - What is This ISO 9000? (1992)

&lt;div class="htmlview paragraph"&gt;The way we look at organizations has changed. Requirements for consistent and high quality at affordable costs while meeting the competition from around the world, have meant that organizations are now more conscious of the need to use all their resources to the best effect. Over the past 20 years various management and organizational systems have come to be known as Quality Management Systems. This paper presents an overview of how the requirements of quality are reflected in organization structures, in both product and service orientated organizations.&lt;/div&gt; &lt;div class="htmlview paragraph"&gt;The basis of the paper is the International Standard series 9000, which has been adopted by many developed nations, coupled with some personal observations.&lt;/div&gt; &lt;div class="htmlview paragraph"&gt;This paper aims for a perspective which stresses the corporate needs for quality organization rather than a detailed prescription.&lt;/div&gt;
Shedding of EGFR is mediated by the ERK MAP kinase pathway (2005)

Proc Amer Assoc Cancer Res, Volume 46, 2005

4504 

We have detected a soluble isoform of EGFR in the conditioned culture media (CCM) of the breast adenocarcinoma cell line, MDA-MB-468. Amino acids sequence analysis of tryptic digest from this EGFR soluble isoform demonstrated that it arises from proteolytic cleavage of the membrane bound receptor. In this regard, proteolytic cleavage of EGFR was recently reported in the vulvar adenocarcinoma cell line A-431 by Zhen et al. (Biochemistry Journal, 42 (18):5478-5492; 2003). We named this protein as proteolytic soluble isoform of EGFR (PI-sEGFR). We have demonstrated that the release of PI-sEGFR into the CCM of MDA-MB-468 cells is stimulated by phorbol 12-myristate 13-acetate (PMA), epidermal growth factor (EGF), transforming growth factor alpha (TGF-a), fetal bovine serum and pervanadate. Moreover, our data suggest that the action of PMA on EGFR shedding is mediated by protein kinase C (PKC). We are interested to study if the action of PKC is mediated through the ERK MAP kinase signaling cascade. In this regard, the ERK MAP kinase signaling cascade mediates the PMA and growth factor induced ectodomain shedding of many transmembrane proteins (Fan H and Derynck R. The EMBO Journal, 18 (24):6962-6972, 1999). To examine the participation of ERK MAP kinase in the EGFR shedding, MDA-MB-468 cells were incubated with the MEK inhibitor, PD 98059 in the presence and absence of PMA. The released of PI-sEGFR was assessed by immunoblot analysis and by acridinium linked immunosorbent assay (ALISA) using antibodies specific for the extracellular domain of EGFR. The inhibition of ERK by PD 98059 was examined by immunoblot analysis using phospho-specific ERK antibodies. The results of these experiments shown that PD 98059 inhibited, as expected, the activation of Erk p42-44 and abolished the PMA-induced shedding of EGFR. Together, our results suggest that the shedding of EGFR in MDA-MB-468 cells is a regulated process and that the ERK MAP kinase signaling cascade might be involved. Supported by the NIH/NCI (CA73859, and CA85133), NIH/MBRS-SCORE (2-806GM08224), and NIH/COBRE (P200RR016439).
Effects of 3' dangling end stacking on the stability of GGCC and CCGG double helixes (1983)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEffects of 3' dangling end stacking on the stability of GGCC and CCGG double helixesSusan M. Freier, Barbara J. Burger, Dirk Alkema, Thomas Neilson, and Douglas H. TurnerCite this: Biochemistry 1983, 22, 26, 6198–6206Publication Date (Print):December 1, 1983Publication History Published online1 May 2002Published inissue 1 December 1983https://pubs.acs.org/doi/10.1021/bi00295a025https://doi.org/10.1021/bi00295a025research-articleACS PublicationsRequest reuse permissionsArticle Views346Altmetric-Citations76LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
ARBlocks: A Concept for a Dynamic Blocks Platform for Educational Activities (2011)

This paper describes the concept of a dynamic blocks platform, called ARBlocks, based on projective augmented reality and tangible user interfaces aiming educational activities. In it, the information is displayed by projectors, that exhibit the content only on the blocks using a projector calibration technique and the blocks are tracked through a frame marker. Despite the platform is still under development, some results regarding the frame marker tracking and the projection was achieved.
In Vitro System To Estimate Renal Brush Border Enzyme-Mediated Cleavage of Peptide Linkages for Designing Radiolabeled Antibody Fragments of Low Renal Radioactivity Levels (2005)

Renal localization of radiolabeled antibody fragments presents a problem in targeted imaging and radiotherapy. We recently reported that Fab fragments labeled with 3'-[(131)I]iodohippuryl N(epsilon)-maleoyl-l-lysine (HML) demonstrated markedly low renal radioactivity levels from early postinjection in mice. Previous studies suggested that low renal radioactivity levels were attributable to cleavage of the glycyl-lysine sequence in HML by the action of renal brush border enzymes, followed by urinary excretion of the resulting m-iodohippuric acid. In this study, an in vitro system using brush border membrane vesicles (BBMVs) isolated from the rat kidney cortex was developed to estimate renal brush border enzyme(s)-mediated cleavage of the peptide linkage. Low molecular weight HML derivatives, 3'-[(125)I]iodohippuryl l-lysine (HL), 3'-[(125)I]iodohippuryl N(epsilon)-tert-butoxycarbonyl-l-lysine (HBL), and their d-amino acid counterparts, were synthesized and incubated in BBMVs. Both [(125)I]HL and [(125)I]HBL generated m-[(125)I]iodohippuric acid after incubation in BBMVs at 37 degrees C while the latter liberated significantly higher amounts of the metabolite. [(125)I]d-HL and [(125)I]d-HBL failed to release the metabolite under similar conditions. The liberation of m-[(125)I]iodohippric acid from [(125)I]HL was significantly facilitated or completely inhibited by the addition of an activator or an inhibitor for carboxypeptidase M. The release of m-[(125)I]iodohippuric acid from [(125)I]HBL increased by the addition of the activator, whereas the inhibitor partially inhibited the release of the metabolite from [(125)I]HBL. The BBMV-mediated release of m-[(125)I]iodohippuric acid from [(125)I]HBL was not impaired by the addition of inhibitors for neutral endopeptidase or renal dipeptidase. These findings showed that the glycyl-l-lysine sequence in HML would be recognized and cleaved by metalloenzymes and nonmetalloenzymes on the renal brush border even when iodine was incorporated into a benzene ring and the N(epsilon)-amine residue of lysine was chemically modified, which supported the hypothesis that low renal radioactivity levels of HML-conjugated Fab fragments would be attributed to the release of m-iodohippuric acid by renal brush border enzymes. This study suggested that this in vitro system using BBMVs would be useful to estimate radiolabeling reagents of antibody fragments or peptides designed to reduce renal radioactivity with a variety of radionuclides.
42% high-efficiency two-stage HBT power-amplifier MMIC for W-CDMA cellular phone systems (2000)

This is the first paper to report on a high-efficiency two-stage heterojunction-bipolar-transistor power-amplifier monolithic microwave integrated circuit (MMIC) for 1.95-GHz wide-band code-division multiple-access (W-CDMA) cellular phone systems. Power amplifiers for W-CDMA systems are required to operate at high efficiency and high linearity over a wide range of output power levels. To obtain high efficiency at low output power (P/sub out/) as well as at the required maximum P/sub out/ and obtain a high linearity at the maximum P/sub out/, we chose near-class-B operation. To improve linearity at a medium P/sub out/ range, we suppressed the gain distortion resulting from near-class-B operation by using an adaptive biasing technique. The MMIC exhibited a power-added efficiency of 42%, the highest ever reported, a gain of 30.5 dB, and an adjacent channel leakage power ratio at a 5-MHz offset frequency of -38 dBc at a P/sub out/ of 27 dBm under a supply voltage of 3.5 V with 3.84-Mcps hybrid phase-shift keying modulation.
Excitation enhancement and extraction enhancement with photonic crystals (2023)

Disclosed herein is a system for stimulating emission from at least one an emitter, such as a quantum dot or organic molecule, on the surface of a photonic crystal comprising a patterned dielectric substrate. Embodiments of this system include a laser or other source that illuminates the emitter and the photonic crystal, which is characterized by an energy band structure exhibiting a Fano resonance, from a first angle so as to stimulate the emission from the emitter at a second angle. The coupling between the photonic crystal and the emitter may result in spectral and angular enhancement of the emission through excitation and extraction enhancement. These enhancement mechanisms also reduce the emitter's lasing threshold. For instance, these enhancement mechanisms enable lasing of a 100 nm thick layer of diluted organic molecules solution with reduced threshold intensity. This reduction in lasing threshold enables more efficient organic light emitting devices and more sensitive molecular sensing.
Metallic Behavior of Cyclotron Relaxation Time in Two-Dimensional Systems (2011)

Cyclotron resonance of two-dimensional electrons is studied at low temperatures down to 0.4 K for a high-mobility Si/SiGe quantum well which exhibits a metallic temperature dependence of dc resistivity ρ. The relaxation time τ(CR) shows a negative temperature dependence, which is similar to that of the transport scattering time τ(t) obtained from ρ. The ratio τ(CR)/τ(t) at 0.4 K increases as the electron density N(s) decreases, and exceeds unity when N(s) approaches the critical density for the metal-insulator transition.
Dkk-1 and IL-7 in plasma of patients with multiple myeloma prevent differentiation of mesenchymal stem cells into osteoblasts. (2014)

Bone disease is the leading cause of morbidity associated with multiple myeloma (MM). Lytic bone lesions have been detected in 90% of patients diagnosed with MM and present a great therapeutic challenge. After the removal of the tumor burden, the bone lesions persist and the bone remodeling homeostasis is not restored even in patients in clinical remission. To determine whether systemic factors generated by malignant MM cells can skew the osteoblast (OB) differentiation program of normal mesenchymal stem cells (MSCs), we generated an immortalized bone marrow MSC line (hTERT-MSC). The hTERT-MSCs were exposed to plasma from healthy donors and patients with MM. Cells grown in media supplemented with plasma from MM patients failed to differentiate into OBs, while the hTERT-MSCs grown in the presence of normal human plasma generated OB clusters that mineralized calcium, expressed Runx2, and were positive for alkaline phosphatase, fibronectin, collagen I, osteocalcin, and osteopontin. Blocking Dickkopf-1 (Dkk-1) and interleukin-7 (IL-7) in MM plasma restored proper OB differentiation of hTERT-MSCs. Finally, we show that hTERT-MSCs cultured in the presence of MM plasma adopt a cancer-associated stroma phenotype. Thus, we show, that systemic factors present in the plasma of patients with MM affect the behavior of non-malignant MSCs and contribute to the sustained bone disease reported in MM.
Measurements of High Temperature Superconductors by Thermal Modulation of Electrical Conductivity. (1990)

The discovery of materials that show superconductivity at a temperature above the boiling point of nitrogen 1 has generated a flood of interest in the area of high temperature superconductivity. The large change in resistance of a superconducting sample at its transition temperature makes it appropriate for analysis by thermal modulation techniques. Studies are presently being performed on the YBa 2 Cu 3 O 7 and Bi-Sr-Ca-Cu-O classes of high temperature superconductors.
Novel Mutation in the Per-Arnt-Sim Domain of KCNH2 Causes a Malignant Form of Long-QT Syndrome (2005)

Our data suggest that besides pore mutations, mutations in the PAS domain may also exhibit a malignant outcome. Pharmacological restoration of current density is promising as a mutation-specific therapy for patients carrying this trafficking-defective mutant.