gebhart/patrepeval huggingface dataset:
Performance modeling of layered communication systems in a multiprocessing environment (1991)

High-bandwidth communication platforms, where transmission rates well exceed 100 Mb/s, are currently the focus of various research, development, and standardization efforts. However, issues related to the design and implementation of complete communication systems capable of efficiently using the full potential of these platforms are not well understood. Consequently, it is essential to develop realistic and complete models for such systems, considering the characteristics of the new environment in which they will be used. The vast majority of the performance studies of communication systems are related to specific protocols and, therefore, do not reach general conclusions about the systems analyzed. Most existing models do not consider end-to-end issues but, rather, concentrate on either independent layer implementations or a set of two or three layers forming just a subset of a larger system. Finally, multiprocessing platforms have not been considered in currently available communication-systems models.
Effects of p53 mutants on wild-type p53-mediated transactivation are cell type dependent. (1995)

The spectrum of p53 mutations differs among human cancer types. We have hypothesized that the p53 mutational spectrum observed in particular tumor types reflects the functional ability of different p53 mutants to modulate wild-type (WT) p53-dependent gene transcription. Missense p53 mutants representing several mutational hotspot codons were cotransfected with WT p53 and analysed for their effects on p53-dependent transactivation of a reporter construct containing a specific p53 binding sequence (PG13-CAT) in human tumor cell lines lacking endogenous p53. Our results show that the ability of p53 mutants to inhibit WT p53-mediated transactivation is cell type dependent. In cell lines derived from a lung adenocarcinoma and a mesothelioma, the transactivation function of WT p53 was strongly inhibited by all p53 mutants examined. However, in cell lines derived from a prostate carcinoma and an osteosarcoma, the mutants examined generally had only minimal dominant negative effects. In cell lines derived from a hepatocellular carcinoma and an ovarian carcinoma, two mutants (248trp and 273his) enhanced WT p53-mediated transactivation of the reporter construct. Additional mutants retained the ability to inhibit WT p53-mediated transactivation in these cell lines. In addition, in a series of four breast tumor cell lines, the p53 mutants examined had similar effects on WT p53 transactivation ability including enhanced transactivation activity in the 273his cotransfectants. The p53 mutants were incapable of transactivating the PG13-CAT reporter in the absence of WT p53 expression. Therefore, the dominant negative effects of p53 mutants on WT p53 function may vary depending on the particular cell type. In addition, mutants with stronger inhibitory capabilities may confer a selective advantage during the tumorigenic process.
Path diversity for FFH/PSK spread-spectrum communication systems (1994)

A fast frequency hopping (FFH) method which uses path-diversity combining is proposed. Diversity techniques are realized when a signal is received from diverse independent paths, each of which carries identical information but suffers from independent fading variations. The improvement of communication performance of FFH systems is possible as the delayed paths are used and path-diversity combination is realized. The advantages of this method, operating in Rayleigh fading channels are confirmed by theoretical analyses. The improvement is in order of 2/spl sim/3 dB at bit error rate (BER) of 10/sup -3/. This method can be also effective against interferences from other users in a code division multiple access (CDMA) environment. The performance of this system in a CDMA environment is evaluated by theoretical analyses and is shown to be superior to non-combining method. At BER of 10/sup -3/ the required E/sub b//N/sub 0/ of the proposed system is 5 dB lower. If E/sub b//N/sub o/ is fixed, a greater number of users can be accommodated using the proposed system.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Detection of Lung Cancer Cells in Solutions Using a Terahertz Chemical Microscope (2021)

Cancer genome analysis has recently attracted attention for personalized cancer treatment. In this treatment, evaluation of the ratio of cancer cells in a specimen tissue is essential for the precise analysis of the genome. Conventionally, the evaluation takes at least two days and depends on the skill of the pathologist. In our group, a terahertz chemical microscope (TCM) was developed to easily and quickly measure the number of cancer cells in a solution. In this study, an antibody was immobilized on a sensing plate using an avidin-biotin reaction to immobilize it for high density and to improve antibody alignment. In addition, as the detected terahertz signals vary depending on the sensitivity of the sensing plate, the sensitivity was evaluated using pH measurement. The result of the cancer cell detection was corrected using the result of pH measurement. These results indicate that a TCM is expected to be an excellent candidate for liquid biopsies in cancer diagnosis.
Efficient algorithm for a quantum analogue of 2-SAT (2011)

Complexity of a quantum analogue of the satisfiability problem is studied. Quantum k-SAT is a problem of verifying whether there exists n-qubit pure state such that its k-qubit reduced density matrices have support on prescribed subspaces. We present a classical algorithm solving quantum 2-SAT in a polynomial time. It generalizes the well-known algorithm for the classical 2-SAT. Besides, we show that for any k>=4 quantum k-SAT is complete in the complexity class QMA with one-sided error.
Method for isolation of human ventricular myocytes from single endocardial and epicardial biopsies (1995)

The study of adult human ventricular cells has been limited by tissue availability. In this study we describe techniques for the isolation of Ca(2+)-tolerant adult human ventricular cells from both transvenous endomyocardial and epicardial biopsies. Ca(2+)-tolerant cells were obtained from 80% of the biopsies processed. Although the yield of Ca(2+)-tolerant myocytes from either type of biopsy was low (1-5%), myocytes with normal resting potentials and action potentials can be obtained from single biopsy specimens, providing a source of normal human myocytes for electrophysiological study. Resting potentials (Vrest) were recorded in 41 isolated right ventricular endomyocardial cells at 37 degrees C. Sixteen cells were depolarized (Vrest = -26 +/- 13 mV), and 25 cells had normal resting potentials (Vrest = -84 +/- 6 mV). Action potentials were recorded in 16 cells. At a pacing cycle length of 1 s, 4 cells had prolonged action potential duration at 90% (APD90, 718 +/- 26 ms) and 10 cells had normal APD90 (381 +/- 94 ms) compared with those recorded from intact right ventricular septal trabeculae from explanted hearts. Voltage-clamp studies of isolated human ventricular myocytes obtained from these biopsies document the presence of currents previously reported from cells isolated from explanted hearts.
Broadband Continuously Frequency Tunable Gyrotron for 600 MHz DNP-NMR Spectroscopy (2014)

A broadband continuously frequency tunable gyrotron with a triode-type magnetron injection gun was developed as power source for analysis of protein structures. The TE7,3 oscillation mode was selected to avoid mode competitions in the high magnetic field side. Axial modes of the TE7,3,−10 were sequentially excited by changing the cavity magnetic field, and frequency tuning of about 4 GHz around 395 GHz was observed with output power greater than 50 W. The frequency also varied about 1GHz as the anode-cathode voltage varied. Thus, the broadest tuning bandwidth in the 400 GHz band gyrotrons was achieved.
The significance of the extracellular matrix in intracranial aneurysms. (1993)

To what extent might change sin the extracellular matrix proteins in intracranial aneurysms (IA) or parent artery (PA) wall contribute to enlargement? Using IA specimens obtained from the coroner's office, the wall structure by light (Trichrome and Actin stains) and immunofluorescent microscopy (IF) (collagen I and fibronectin) have been studied in 18 specimens. It is well known that IA only arise when there is a deficiency in the internal elastic membrane and some breakdown in the media. The results show a normal three layer structure of intima, media, (muscularis), and adventitia in the PA extending out into the neck of the aneurysm. Immunofluorescent stains (IS) show collagen I and fibronectin limited to the adventitia and muscularis, respectively, of the PA and control arteries. The enlarging IA wall shows almost complete loss of this architecture. By IF stain, collagen I and fibronectin are intermingled throughout the IA wall. It is postulated that loss of structure and the confining muscularis layer permit easier elastic enlargement from a more or less homogeneous wall structure.
Angiopeptin-eluting stents: observations in human vessels and pig coronary arteries. (2002)

Local drug delivery from polymer-coated coronary stents may reduce the incidence of in-stent restenosis. Angiopeptin, an inhibitor of smooth muscle cell proliferation, may reduce the clinical impact of restenosis. The objectives of this study were to characterize the release kinetics and distribution of angiopeptin-loaded phosphorylcholine (PC)-coated drug delivery (DD) BiodivYsio stents and assess their safety and efficacy at reducing neointima formation. I125-angiopeptin-loaded DD-PC-coated stents were implanted into human saphenous vein segments ex vivo, and I125 angiopeptin was detected in the medial layer at 1 hour. When implanted in pig coronary arteries, I125 angiopeptin was found adjacent to the stent at intervals up to 28 days. No significant amounts were found elsewhere. To assess efficacy, twelve angiopeptin-loaded DD-PC-coated stents, twelve non-loaded DD-PC stents, ten standard PC-coated stents and 8 uncoated stents were implanted into normal porcine coronary arteries. Stents were harvested at 28 days and neointima formation was assessed by computerized morphometry. No adverse tissue reactions were seen with any of the PC-coated stents. No significant differences were seen in neointimal or luminal cross-sectional areas between study groups. Local delivery of I125 angiopeptin into the vascular wall can be achieved using a PC-coated stent. Delivery of angiopeptin from drug delivery PC-coated stents is safe, but does not lead to a significant reduction in neointimal growth at 28 days within the parameters of this study.
Clinical Validity of Integrated Molecular Profiling in Diagnosing Pancreatic Lesions: Interim Results (N=422) of an Outcomes-based Registry (2013)

Purpose: A multi-institutional review of patient medical records was performed in order to determine the clinical validity (performance characteristics and risk stratification) of PathfinderTG (PFTG, Red-Path Integrated Pathology) when ordered clinically as a second line test. Methods: Four hundred twenty-two patients previously tested with PFTG had outcomes that met study criteria. Patient outcomes were classified into two groups (benign or malignant) based on surgical pathology (n=195), inequivocally diagnostic cytology (n=20), evidence of clinical cancer treatment (n=5) or at least 2 years of clinical and imaging follow up (n=202). All PFTG analyses were ordered according to practitioner standard of care. PFTG diagnoses were classified into four distinct categories: benign, statistically indolent (SI), statistically high risk (SHR), and aggressive based on predetermined molecular, radiographic and clinical criteria. For the purpose of calculating performance characteristics, diagnoses of benign and SI were treated as negative for malignancy (benign), while SHR and aggressive were treated as positive for malignancy. Results: PFTG had a sensitivity and specificity for malignancy of 81% [95% confidence interval [CI] 70-89%] and 91% [95% CI 88-94%], respectively, with an overall accuracy 90% [95% CI 86-92%]. Testing also showed a negative predictive value (NPV) of 96% [95% CI 93-98] and a positive predictive value of 64% [95% CI 53-74%]. Relative to patients with benign diagnoses, patients with SHR and Aggressive diagnoses were 25.95 (p<0.0001) and 51.75 (p<0.0001), respectively more likely to get malignancy. Conclusion: When first line testing results are not definitive, second line PFTG testing can provide clinically valid diagnostic and predictive information that may help to better manage patients. PFTG is able to predict benign and statistically indolent disease in patients with high specificity. Furthermore, PFTG can identify patients with and at higher risk of malignancy (SHR and Aggressive). Disclosure - Brendan Corcoran: Employee:RedPath Integrated Pathology; Eric Ellsworth: Employee: RedPath Integrated Pathology; Sara Jackson: Employee:RedPath Integrated Pathology; Sydney Finkelstein, MD: Founder, Board Member:RedPath Integrated Pathology. This research was supported by an industry grant from RedPath Integrated Pathology.Figure: Kaplan-Meier curve representing the four diagnostic categories of PathFinder TG.
ASSOCIATION OF NEWLY FORMED VIRAL PROTEIN WITH SPECIFIC POLYRIBOSOMES (1963)

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans the biological, physical, and social sciences.
Cigarette Smoke Exposure Exacerbates Lung Inflammation and Compromises Immunity to Bacterial Infection (2014)

The detrimental impact of tobacco on human health is clearly recognized, and despite aggressive efforts to prevent smoking, close to one billion individuals worldwide continue to smoke. People with chronic obstructive pulmonary disease are susceptible to recurrent respiratory infections with pathogens, including nontypeable Haemophilus influenzae (NTHI), yet the reasons for this increased susceptibility are poorly understood. Because mortality rapidly increases with multiple exacerbations, development of protective immunity is critical to improving patient survival. Acute NTHI infection has been studied in the context of cigarette smoke exposure, but this is the first study, to our knowledge, to investigate chronic infection and the generation of adaptive immune responses to NTHI after chronic smoke exposure. After chronic NTHI infection, mice that had previously been exposed to cigarette smoke developed increased lung inflammation and compromised adaptive immunity relative to air-exposed controls. Importantly, NTHI-specific T cells from mice exposed to cigarette smoke produced lower levels of IFN-γ and IL-4, and B cells produced reduced levels of Abs against outer-membrane lipoprotein P6, with impaired IgG1, IgG2a, and IgA class switching. However, production of IL-17, which is associated with neutrophilic inflammation, was enhanced. Interestingly, cigarette smoke-exposed mice exhibited a similar defect in the generation of adaptive immunity after immunization with P6. Our study has conclusively demonstrated that cigarette smoke exposure has a profound suppressive effect on the generation of adaptive immune responses to NTHI and suggests the mechanism by which prior cigarette smoke exposure predisposes chronic obstructive pulmonary disease patients to recurrent infections, leading to exacerbations and contributing to mortality.
Functional comparison between genomic subtypes and protein signal transduction networks in metastatic triple-negative breast cancer tumor and stroma to identify actionable therapeutic strategies. (2014)

e22155 Background: Genomic sequencing has been conducted on primary TNBC, however it has been difficult to identify driver mutations that constitute actionable therapy strategies. Importantly, genomic profiling of primary TNBC may not correspond to the lethal metastatic mTNBC lesions. We conducted extensive proteomic signal pathway network analysis of microdissected tumor cells and stroma from mTNBC that have been subjected to full genomic sequencing. We addressed the following questions: 1) Can potential driver mutations in the metastasis be inferred from protein signaling pathways in the tumor cells? 2) Does the tumor cell signal pathway network reflect differences in local mTNBC versus the distant organ mTNBC? 3) Does the proteomic signaling profile of the stroma provide strategies for stromal therapy of mTNBC? We hypothesize that the functional comparison of the genomic subtype with the protein signal transduction pathways within the tumor-stromal microenvironment of the metastasis may provide novel therapeutic targets for TNBC. Methods: Whole genome and transcript sequencing was compared to 130 signal transduction proteins in the microdissected tumor and stroma of 13 mTNBC frozen samples, representing lymph node and local (n=7) and distant soft tissue (n=6) metastasis. Cell signaling pathway proteins were quantified by reverse phase protein microarray. Linkage analysis was done by non-parametric Spearman rho (rho>0.8, p<0.03) pair wise correlation. Results: Comparison of the tumor and stroma signaling network of local versus distant metastasis revealed that the stroma of distant metastasis were dominated by mTOR Ser2448/PTEN Ser380/HER4/SGK1 signaling (rho=0.9). Distant tumor signaling was dominated by STAT3 Ser727/Beclin1/AKT Thr308/COX2/TGFβ (rho=0.9). In contrast, stroma of local metastasis lacked the mTOR pathway linkages but exhibited HIF-1α-MET/Tyr1234/EGFR Ty1148 linkages (rho=0.9). Local tumor protein pathway linkages were similar to distant tumor signaling. Conclusions: Profiling mTNBC stromal signaling networks provides novel targets for new classes of stromal therapy.
Multiple bonds between main-group elements and transition metals. 91. High-oxidation-state rhenium complexes containing the hydridotris(1-pyrazolyl)borato ligand (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMultiple bonds between main-group elements and transition metals. 91. High-oxidation-state rhenium complexes containing the hydridotris(1-pyrazolyl)borato ligandIan A. Degnan, Joachim Behm, Malcolm R. Cook, and Wolfgang A. HerrmannCite this: Inorg. Chem. 1991, 30, 9, 2165–2170Publication Date (Print):May 1, 1991Publication History Published online1 May 2002Published inissue 1 May 1991https://pubs.acs.org/doi/10.1021/ic00009a039https://doi.org/10.1021/ic00009a039research-articleACS PublicationsRequest reuse permissionsArticle Views81Altmetric-Citations37LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Experimental Investigations and Numerical Modeling of Incompressible Elastomers during Non-Homogeneous Deformations (1998)

Abstract Three hyperelastic constitutive models, the Arruda and Boyce model, the Yeoh model, and the Ogden model, are used to simulate the response of vinyl elastomers in large, non-homogeneous deformation states. A single uniaxial compression test is used to characterize the elastomers to obtain model constants. Drucker stability is enforced by restriction of the coefficients for the Yeoh and Ogden models to produce physically feasible uniaxial results. The ability of the models to predict the global force versus deformation responses and deformed shapes for large deformation shear, tensile deformation of a long bar with fixed grips, and inflation of a thin disk is examined. The experiments used in this study are designed to provide known boundary conditions to eliminate ambiguity in the modeling. We demonstrate for the first time that each of these models may be sufficiently characterized via a simple, homogeneous compression test to allow accurate predictions of large, non-homogeneous deformations involving rotations of the principal stretch directions.
Chemoenzymatic Synthesis of Thiazolyl Peptide Natural Products Featuring an Enzyme-Catalyzed Formal [4 + 2] Cycloaddition (2020)

Thiocillins from Bacillus cereus ATCC 14579 are members of the well-known thiazolyl peptide class of natural product antibiotics, the biosynthesis of which has recently been shown to proceed via posttranslational modification of ribosomally encoded precursor peptides. It has long been hypothesized that the final step of thiazolyl peptide biosynthesis involves a formal [4 + 2] cycloaddition between two dehydroalanines, a unique transformation that had eluded enzymatic characterization. Here we demonstrate that TclM, a single enzyme from the thiocillin biosynthetic pathway, catalyzes this transformation. To facilitate characterization of this new class of enzyme, we have developed a combined chemical and biological route to the complex peptide substrate, relying on chemical synthesis of a modified C-terminal fragment and coupling to a 38-residue leader peptide by means of native chemical ligation (NCL). This strategy, combined with active enzyme, provides a new chemoenzymatic route to this promising class of antibiotics.
On Multiagent Exploration (1998)

This paper describes a technique for multi-agent exploration of an unknown environment, that improves the quality of the map by reducing the inaccuracies that occur over time from dead reckoning errors. We present an algorithmic solution, simulation results, as well as a cost analysis and experimental data. The approach is based on using a pair of robots that observe one another’s behaviour, thus greatly reducing odometry errors. We assume the robots can both directly sense nearby obstacles and see one another. We have implemented both these capabilities with actual robots in our lab. By exploiting the ability of the robots to see one another, we can detect opaque obstacles in the environment independent of their surface reflectance properties. 1 1