gebhart/patrepeval huggingface dataset:
Differential Effects of Physiologically Relevant Hypoxic Conditions on T Lymphocyte Development and Effector Functions (2001)

Direct measurements revealed low oxygen tensions (0.5-4.5% oxygen) in murine lymphoid organs in vivo. To test whether adaptation to changes in oxygen tension may have an effect on lymphocyte functions, T cell differentiation and functions at varying oxygen tensions were studied. These studies show: 1) differentiated CTL deliver Fas ligand- and perforin-dependent lethal hit equally well at all redox conditions; 2) CTL development is delayed at 2.5% oxygen as compared with 20% oxygen. Remarkably, development of CTL at 2.5% oxygen is more sustained and the CTL much more lytic; and 3) hypoxic exposure and TCR-mediated activation are additive in enhancing levels of hypoxia response element-containing gene products in lymphocyte supernatants. In contrast, hypoxia inhibited the accumulation of nonhypoxia response element-containing gene products (e.g., IL-2 and IFN-gamma) in the same cultures. This suggests that T cell activation in hypoxic conditions in vivo may lead to different patterns of lymphokine secretion and accumulation of cytokines (e.g., vascular endothelial growth factor) affecting endothelial cells and vascular permeabilization. Thus, although higher numbers of cells survive and are activated during 20% oxygen incubation in vitro, the CTL which develop at 2.5% oxygen are more lytic with higher levels of activation markers. It is concluded that the ambient 20% oxygen tension (plus 2-ME) is remarkably well suited for immunologic specificity and cytotoxicity studies, but oxygen dependence should be taken into account during the design and interpretation of results of in vitro T cell development assays and gene expression studies in vivo.
Topics in This Issue: Cancer Testes Antigens, Immune Checkpoints, Inflammation Associated with Ischemia-Reperfusion and Integrin Targeting (2012)

This issue of the International Reviews of Immunology is dedicated to several topics: cancer immunotherapy, and basic and translational aspects of immunity. Two reviews, one focused on breast and the other on lung cancer, highlight the need to redefine the cancer testes antigens (CTAs) as novel information regarding their expression profile and biological role emerges. Two other reviews showcase pivotal molecules that keep in check immunity at two different levels: the transcription factor autoimmune regulator (AIRE) important to negative selection of the T-cell repertoire, and CD22 that limits the antigen-initiated B-cell response. Two other articles focus on the debated role of Toll-like receptors (TLRs) and inflammation in general, in ischemia-reperfusion lesions that follow cardiovascular disorders and stroke. Last but not the least, this issue hosts a review that discusses the role and translational potential of the α4 integrin for the treatment of inflammatory bowel disease (IBD).
General Procedure for Acylation of 3° Alcohols:  Scandium Triflate/DMAP Reagent (1998)

ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTGeneral Procedure for Acylation of 3° Alcohols: Scandium Triflate/DMAP ReagentHong Zhao, Annapurna Pendri, and Richard B. GreenwaldView Author Information Enzon, Inc., 20 Kingsbridge Road, Piscataway, New Jersey 08854 Cite this: J. Org. Chem. 1998, 63, 21, 7559–7562Publication Date (Web):September 24, 1998Publication History Received16 June 1998Published online24 September 1998Published inissue 1 October 1998https://pubs.acs.org/doi/10.1021/jo981161chttps://doi.org/10.1021/jo981161cbrief-reportACS PublicationsCopyright © 1998 American Chemical SocietyRequest reuse permissionsArticle Views3743Altmetric-Citations79LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Alcohols,Organic compounds,Organic reactions,Post-translational modification,Reagents Get e-Alerts
Supplementary Figure 2 from <i>In Vivo</i> Persistence, Tumor Localization, and Antitumor Activity of CAR-Engineered T Cells Is Enhanced by Costimulatory Signaling through CD137 (4-1BB) (2023)

Supplementary Figure 2 from &lt;i&gt;In Vivo&lt;/i&gt; Persistence, Tumor Localization, and Antitumor Activity of CAR-Engineered T Cells Is Enhanced by Costimulatory Signaling through CD137 (4-1BB)
Supported Au nanoparticles as efficient catalysts for aerobic homocoupling of phenylboronic acid (2012)

Au nanoparticles with small sizes (1-4 nm) were effectively formed on Mg-Al mixed oxides (Au/MAO), which showed superior catalytic performances and good recyclability in aerobic homocoupling of phenylboronic acid.
Supplementary Methods from ERK Inhibitor LY3214996 Targets ERK Pathway–Driven Cancers: A Therapeutic Approach Toward Precision Medicine (2023)

&lt;p&gt;Supplementary Methods&lt;/p&gt;
A Simple and Non-Invasive Method for Nuclear Transformation of Intact-walled Chlamydomonas reinhardtii (2014)

Genetic engineering in microalgae is gaining attraction but nuclear transformation methods available so far are either inefficient or require special equipment. In this study, we employ positively charged nanoparticles, 3-aminopropyl-functionalized magnesium phyllosilicate (aminoclay, approximate unit cell composition of [H2N(CH2)3]8Si8Mg6O12(OH)4), for nuclear transformation into eukaryotic microalgae. TEM and EDX analysis of the process of transformation reveals that aminoclay coats negatively-charged DNA biomolecules and forms a self-assembled hybrid nanostructure. Subsequently, when this nanostructure is mixed with microalgal cells and plated onto selective agar plates with high friction force, cell wall is disrupted facilitating delivery of plasmid DNA into the cell and ultimately to the nucleus. This method is not only simple, inexpensive, and non-toxic to cells but also provides efficient transformation (5.03×10(2) transformants/µg DNA), second only to electroporation which needs advanced instrumentation. We present optimized parameters for efficient transformation including pre-treatment, friction force, concentration of foreign DNA/aminoclay, and plasticity of agar plates. It is also confirmed the successful integration and stable expression of foreign gene in Chlamydomonas reinhardtii through molecular methods.
Solution Properties of a Fluorinated Alkyl Methacrylate Polymer in Carbon Dioxide (2006)

The solution properties of a fluorinated alkyl methacrylate, poly(1,1,2,2-tetrahydroperfluorooctyl methacrylate) (PFOMA), in liquid and supercritical carbon dioxide (CO2) were studied by static and dynamic light scattering. The solvent quality of CO2 was found to improve with increasing temperature and CO2 density as exhibited by an increase of the second virial coefficient. Both the hydrodynamic radius expansion factor and the second virial coefficient of PFOMA solution were found to be functions of a single interaction parameter that can be independently changed by either temperature or density variations. Furthermore, we demonstrate that the relationship between two directly measurable quantities, the second virial coefficient and the hydrodynamic expansion ratio, is the same for both temperature-induced and CO2 density-induced variations of solvent quality.
IκBε provides negative feedback to control NF-κB oscillations, signaling dynamics, and inflammatory gene expression (2006)

NF-kappaB signaling is known to be critically regulated by the NF-kappaB-inducible inhibitor protein IkappaBalpha. The resulting negative feedback has been shown to produce a propensity for oscillations in NF-kappaB activity. We report integrated experimental and computational studies that demonstrate that another IkappaB isoform, IkappaBepsilon, also provides negative feedback on NF-kappaB activity, but with distinct functional consequences. Upon stimulation, NF-kappaB-induced transcription of IkappaBepsilon is delayed, relative to that of IkappaBalpha, rendering the two negative feedback loops to be in antiphase. As a result, IkappaBepsilon has a role in dampening IkappaBalpha-mediated oscillations during long-lasting NF-kappaB activity. Furthermore, we demonstrate the requirement of both of these distinct negative feedback regulators for the termination of NF-kappaB activity and NF-kappaB-mediated gene expression in response to transient stimulation. Our findings extend the capabilities of a computational model of IkappaB-NF-kappaB signaling and reveal a novel regulatory module of two antiphase negative feedback loops that allows for the fine-tuning of the dynamics of a mammalian signaling pathway.
Least-Squares Optimal Contrast Limited Histogram Equalisation (2019)

Contrast Limited Histogram Equalisation moves the input image histogram gently towards one which has a more uniform distribution. Viewed as a tone mapping operation, CLHE generates a tone curve with bounded max and min slopes. It is this boundedness which ensures that the processed images have more detail but few artefacts. Outside of limiting contrast, recent improvements to histogram equalisation include constraining the tone curve to make good whites and blacks and constraining the tone curve to be smooth. This paper makes three contributions. First, we show that the CLHE formalism is not least-squares optimal but optimality can be achieved by reformulating the problem in a quadratic programming framework. Second, we incorporate the additional constraints of tone curve smoothness and good whites and blacks in our quadratic programming CLHE framework. Third, experiments demonstrate the utility of our method.
One size doesn’t fit all: unraveling the diversity of factors and interactions that drive E. coli urovirulence (2017)

Urinary tract infections (UTIs) are common (with 11 million cases annually in the USA), costly (with $5 billion spent per year), and increasingly antibiotic resistant (1). The clinical manifestations and symptomatologies of UTIs are strikingly complex and result from the interactions between diverse uropathogens and host urothelial tissues. The most common cause of UTIs is UPEC, which cause up to 80% of community-acquired UTIs and 65% of hospital-acquired UTIs (1). Despite appropriate treatment for the initial infection, UTIs are frequently recurrent as 25–35% of initial infections are followed by a recurrent UTI (rUTI) (2). In addition, the high level of same-strain recurrences, in which a single bacterial strain (as defined by genetic identity) causes two or more consecutive UTIs, strongly suggests that there exist non-pathogenic reservoirs within hosts (2). One established reservoir is the gastrointestinal tract (GIT) microbiota (3). UPEC strains can maintain non-pathogenic colonization of the GIT, where they form a reservoir and subsequently seed extraintestinal infections such as UTI. In this review, we detail critical gaps in our understanding of the pathogenic and non-pathogenic colonization of UPEC in different habitats in the host and describe a new perspective on UTI susceptibility that better reflects the complexity of this disease.
Computationally-guided optimization of small-molecule inhibitors of the Aurora A kinase–TPX2 protein–protein interaction (2017)

Free energy perturbation theory, in combination with enhanced sampling of protein-ligand binding modes, is evaluated in the context of fragment-based drug design, and used to design two new small-molecule inhibitors of the Aurora A kinase-TPX2 protein-protein interaction.
<title>Progress on the Development of Luminescent Solar Concentrators</title> (1980)

Various planar configurations of the luminescent solar concentrator (LSC) are discussed including: the uniformly doped, the stacked plate, the thin film, and the multilayered film LSC. Radiation which is lost from the luminescent plate by falling within the critical angle for total internal reflection is examined in terms of the above configurations. A ten parameter efficiency profile has been developed to better evaluate the collector performance and to allow direct comparison with other photovoltaic devices. A collector efficiency of 3.2% is reported and the feasibility of 8-10% efficient devices discussed. Several optical distribution factors have been defined and experimentally evaluated for the luminescent plate and associated photovoltaic cell. These factors have been used to calculate a plate-to-cell optical coupling coefficient for two commercially available solar cells. LSC&apos;s based on organic and inorganic phosphor systems are compared and the problem of dye stability discussed.
Processing of Iridium and Iridium Alloys (2008)

Iridium and its alloys have been considered to be difficult to fabricate due to their high melting temperatures, limited ductility, sensitivity to impurity content and particular chemical properties. The variety of processing methods used for iridium and its alloys are reviewed, including purification, melting, forming, joining and powder metallurgy techniques. Also included are coating and forming by the methods of electroplating, chemical and physical vapour deposition and melt particle deposition.
Power factor correction using an enhancement-mode SiC JFET (2008)

The conventional wisdom that the SiC JFET is a normally on device has recently been superseded by the first practical normally off SiC JFET. The new true enhancement mode, three-terminal, pure-SiC design provides designers with a normally off solution that retains all the benefits of the normally on SiC JFET. With a simple change in the series gate impedance, the EM SiC JFET can be used with common IC drivers and is a drop-in replacement for current power devices in most applications. Device characteristics are superior to MOSFETs and IGBTs and offer the possibility of efficiency improvements from reduced conduction and switching losses. The work presented in this paper demonstrates the drop-in replacement of an IGBT with a normally off SiC JFET in a PFC demo circuit. System efficiency using each device was observed and compared. An improvement was noted with the JFET as expected.
Low power 3D-integrated Solid-State Drive (SSD) with adaptive voltage generator (2010)

A 3D-integrated Solid-State Drive (SSD with an adaptive program-voltage generator is introduced. The proposed boost converter is composed of a spiral inductor, a high-voltage MOS circuit, and an adaptive-frequency and duty-cycle (AFD) controller. The 5 × 5 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> spiral inductor is implemented in an interposer. The high-voltage MOS circuit is fabricated with a matured NAND flash process. The AFD controller is manufactured with a conventional 0.18 um low-voltage CMOS process. The AFD controller dynamically optimizes clock frequencies and duty cycles at different values, depending on the output voltage. As a result, the power consumption, rising time, and circuit area of the program-voltage generator decreases by 88%, 73%, and 85%, respectively. The total power consumption of the NAND flash memory decreases by 68%.
Sporadic breast cancer patients' germline DNA exhibit an AT‐rich microsatellite signature (2011)

Using a custom CGH-like oligonucleotide array to measure the global microsatellite content in the genomes of 72 cancer, cancer-free, and high risk patient and cell line samples (56 germline DNA and 16 in tumor or tumor cell line DNA) we found a unique, reproducible, and statistically significant pattern of 18 motif-specific microsatellite families (out of 962 possible 1-6 mer repeats) in breast cancer patient germline and tumor DNA, but not in germline DNA of cancer-free volunteer controls or in breast cancer patients with BRCA1/2 mutations. These high-similarity A/T rich repetitive motifs were also more pronounced in the germlines and tumors of colon cancer tumor patients (3/6 samples) and microsatellite unstable colon cancer cell lines; however, germline DNA of sporadic breast cancer patients exhibited the largest global content shift for those motifs with extreme AT/GC ratios. These results indicate that global microsatellite variability is complex, suggest the existence of a previously unknown genomic destabilization mechanism in breast cancer patients' germline DNA, and warrant further testing of such microsatellite variability as a predictor of future breast cancer development.
Development of a library of low-loss silicon-on-insulator optoelectronic devices (1996)

Established silicon microelectronics fabrication methods can be exploited to fabricate low-loss silicon integrated optical devices of dimensions which are compatible with single mode fibres and operate at the important wavelengths of 1.3 and 1.5 µm. Recent advances at the University of Surrey, where research effort in this field has been to develop a library of optoelectronic components, is highlighted. So far, rib and planar waveguides, including bends and Y-junctions, have been produced, together with design and fabrication of efficient optical modulators based on the plasma dispersion effect. Within the experimental error, the waveguide losses are indistinguishable from the intrinsic absorption loss of pure silicon. The figure of merit for the modulators is greater than 200°/V/mm and the operating current is 7 mA, which improves on previous devices by approximately an order of magnitude.
7T MR Thermometry technique for validation of system‐predicted SAR with a home‐built radiofrequency wrist coil (2020)

A highly accurate MRT technique with high spatial and temporal resolution was developed. This technique can be used to ensure that system-predicted SAR values are not exceeded in practice, thereby providing independent validation of SAR levels delivered by a newly built RF wrist coil. The MRT technique is readily generalizable to perform safety evaluations for other RF coils at 7T.
Magnetic Gold Confined in Ordered Mesoporous Titania Thin Films: A Noble Approach for Magnetic Devices (2016)

In the past decade, the surprising magnetic behavior of gold nanoparticles has been reported. This unexpected property is mainly attributed both to size and surface effects. Mesoporous thin films are ideal matrices for metallic nanoparticles inclusion, because of their highly accessible and tailorable pore systems that lead to completely tunable chemical environments. Exploiting these features, we synthesized Au nanoparticles within mesoporous titania thin films (film thickness of ∼150 nm and pore diameter of ∼5 nm), and we studied their magnetic properties under confinement. Here, we present the results of the magnetization as a function of temperature and magnetic field for this system, which are consistent with the previously reported for free (unconfined) thiol-capped gold nanoparticles. The successful inclusion of stable magnetic Au nanoparticles within transparent mesoporous thin films opens the gates for the application of these nanocomposites in two-dimensional (2D) microdevices technology and magneto-optical devices.
Low-power pervasive wi-fi connectivity using WiScan (2015)

Pervasive Wi-Fi connectivity is attractive for users in places not covered by cellular services (e.g., when traveling abroad). However, the power drain of frequent Wi-Fi scans undermines the device's battery life, preventing users from staying always connected and fetching synced emails and instant message notifications (e.g., WhatsApp). We study the energy overhead of scan and roaming in detail and refer to it as the scan tax problem. Our findings show that the main processor is the primary culprit of the energy overhead. We propose a simple and effective architectural change of offloading scans to the Wi-Fi radio. We design and build WiScan to fully exploit the gain of scan offloading. Our experiments demonstrate that WiScan achieves 90%+ of the maximal connectivity, while saving 50-62% energy for seeking connectivity.
Child wasting and concurrent stunting in low- and middle-income countries (2020)

Summary Sustainable Development Goal 2.2, to end malnutrition by 2030, includes elimination of child wasting, defined as weight-for-length more than 2 standard deviations below international standards. Prevailing methods to measure wasting rely on cross-sectional surveys that cannot measure onset, recovery, and persistence — key features that inform preventive interventions and disease burden estimates. We analyzed 21 longitudinal cohorts to show wasting is a highly dynamic process of onset and recovery, with incidence peaking between birth and 3 months. By age 24 months 29.2% of children had experienced at least one wasting episode, more than 5-fold higher than point prevalence (5.6%), demonstrating that wasting affects far more children than can be inferred through cross-sectional surveys. Children wasted before 6 months had faster recovery and shorter episodes than children wasted at older ages, but early wasting increased the risk of later growth faltering, including concurrent wasting and stunting (low height-for-age), increasing their risk of mortality. In diverse populations with high seasonal rainfall, population average weight-for-length varied substantially (&gt;0.5 z in some cohorts), with the lowest mean Z-scores during the rainiest months, creating potential for seasonally targeted interventions. Our results elevate the importance of establishing interventions to prevent wasting from birth to age 6 months, likely through improved maternal nutrition, to complement current programs that focus on children ages 6-59 months.
Periodic Conductivity as a Mechanism for Cardiac Stimulation and Defibrillation (1987)

This study examines the distribution of the transmembrane potential in the periodic strand of cardiac muscle established by configurations of sources similar to those arising during extracellular stimulation and defibrillation, during intracellular stimulation, and during propagation of action potential. The closed-form solution indicates that during extracellular stimulation with large current and during defibrillation, the periodic component of the transmembrane potential is very important. We postulate that this periodic component causes the depolarization or defibrillation in cardiac muscle, which is different from the depolarization mechanism for a continuous fiber. On the other hand, during propagation and intracellular stimulation, the periodic component only slightly modifies the monotonic decrease of the transmembrane potential, which suggests that the mechanism of propagation in discrete structures may be similar to that of the continuous fiber.