gebhart/patrepeval huggingface dataset:
Rapid conditional knock-down–knock-in system for mammalian cells (2006)

RNA interference (RNAi) is a powerful tool to analyze gene function in mammalian cells. However, the interpretation of RNAi knock-down phenotypes can be hampered by off-target effects or compound phenotypes, as many proteins combine multiple functions within one molecule and coordinate the assembly of multimolecular complexes. Replacing the endogenous protein with ectopic wild-type or mutant forms can exclude off-target effects, preserve complexes and unravel specific roles of domains or modifications. Therefore, we developed a rapid-knock-down-knock-in system for mammalian cells. Stable polyclonal cell lines were generated within 2 weeks by simultaneous selection of two episomal vectors. Together these vectors mediated reconstitution and knock-down in a doxycycline-dependent manner to allow the analysis of essential genes. Depletion was achieved by an artificial miRNA-embedded siRNA targeting the untranslated region of the endogenous, but not the ectopic mRNA. To prove effectiveness, we tested 17 mutants of WDR12, a factor essential for ribosome biogenesis and cell proliferation. Loss-off function phenotypes were rescued by the wild-type and six mutant forms, but not by the remaining mutants. Thus, our system is suitable to exclude off-target effects and to functionally analyze mutants in cells depleted for the endogenous protein.
Nombres y símbolos en español de los elementos con números atómicos 113, 115, 117 y 118 aceptados por la IUPAC el 28 de noviembre de 2016 (2016)

El 8 de junio de 2016, la IUPAC (International Union of Pure and Applied Chemistry) presento los simbolos y nombres de los elementos 113, 115, 117 y 118 adoptados provisionalmente y que sometio a exposicion publica durante cinco meses para su aprobacion definitiva el 8 de noviembre de 2016. Tras la revision de un gran numero de propuestas y sugerencias enviadas por distintos colectivos, el 28 de noviembre de 2016, la IUPAC ha aceptado definitivamente los nombres y simbolos propuestos por sus descubridores
Suicide, Satisfaction With Life, and Insight Capacity Among Adolescents With Mental Disorders (2016)

Among adolescents with mental disorders, insight is a risk factor, whereas satisfaction with life is a protective factor for suicidality. Insight affects suicide risk of adolescents with mental disorders via reduction of satisfaction with life.
Factorized Similarity Learning in Networks (2014)

The problem of similarity learning is relevant to many data mining applications, such as recommender systems, classification, and retrieval. This problem is particularly challenging in the context of networks, which contain different aspects such as the topological structure, content, and user supervision. These different aspects need to be combined effectively, in order to create a holistic similarity function. In particular, while most similarity learning methods in networks such as Sim Rank utilize the topological structure, the user supervision and content are rarely considered. In this paper, a Factorized Similarity Learning (FSL) is proposed to integrate the link, node content, and user supervision into an uniform framework. This is learned by using matrix factorization, and the final similarities are approximated by the span of low rank matrices. The proposed framework is further extended to a noise-tolerant version by adopting a hinge-loss alternatively. To facilitate efficient computation on large scale data, a parallel extension is developed. Experiments are conducted on the DBLP and CoRA datasets. The results show that FSL is robust, efficient, and outperforms the state-of-the-art.
Breaking the tert-Butyllithium Contact Ion Pair:A Gateway to Alternate Selectivity in Lithiation Reactions (2022)

The selective functionalization of saturated oxygen heterocycles at positions remote to the embedded heteroatoms remains an outstanding challenge in organic synthesis. Although many methods exist for the undirected replacement of C–H bonds with heteroatomic subunits the number of site selective C–H functionalization reactions for the introduction of carbon-carbon bonds pales in comparison. This paper describes the initial stages of a long-term program aimed at elucidating how organolithium reagents can be re-engineered to selectively deprotonate and functionalize saturated heterocycles at new locations. Through rigorous NMR spectroscopic investigations, it was determined for the first time that the addition of Lewis basic phosphoramides can shift the equilibrium of strong organolithium bases, such as t-BuLi, to include the triple ion pair (t-Bu–Li–t-Bu) / L4Li which serves as a reservoir for the highly reactive separated ion pair t-Bu / L4Li . Because the Li-atom’s valences are saturated the Lewis acidity is significantly decreased and the basicity is maximized which allowed for the typical directing effects within oxygen heterocycles to be overridden and for remote sp3-CH bonds to be deprotonated. In certain cases, this enabled the removal of stronger C–H bonds in the presence of weaker C–H bonds. Furthermore, these newly accessed lithium aggregation states were leveraged to develop a simple γ-lithiation and capture protocol (lithium nucleophilic coupling – “LiNC”) of chromane heterocycles with a variety of alkyl halide electrophiles in good yields.
Convergence Properties of Solitary Tract Neurons Responsive to Cardiac Receptor Stimulation in the Anesthetized Cat (1998)

The convergence pattern of cardiac receptors, pulmonary C-fibers, carotid chemoreceptor, and baroreceptor afferents onto neurons within the nucleus of the solitary tract (NTS) was studied in the anesthetized (pentobarbitone sodium, 40 mg/kg,) paralyzed and artificially ventilated cat. Extra- and intracellular recordings were made from NTS neurons while stimulating both cardiac receptors by aortic root injections of veratridine (1-3 micrograms/kg) and pulmonary C-fibers by a right atrial injection of phenylbiguanide (10-20 micrograms/kg). The ipsilateral carotid body was stimulated by using arterial injection of CO2-saturated bicarbonate solution, whereas inflation of the ipsilateral carotid sinus was used to activate baroreceptors. The ipsilateral cardiac vagal branch, cervical vagus, and carotid sinus nerves were stimulated electrically (1 Hz, 0.2-1 ms, 1-35 V). In 78 NTS neurons recorded either extracellularly (n = 47) or intracellularly (n = 31), electrical stimulation of the cardiac branch of the vagus nerve evoked synaptic potentials (spikes and/or excitatory postsynaptic potentials) with an onset latency between 4 and 220 ms. Some neurons displayed both short and long latency inputs(15.5 +/- 1.8 and 160.0 +/- 8.5 ms; n = 14). Of these 78 neurons, 24 responded to veratridine stimulation of cardiac receptors (i.e., cardioreceptive neurons) by exhibiting an augmenting-decrementing discharge of 37 +/- 4 s in duration with a peak frequency of 30 +/- 5 Hz. Convergence from other cardiorespiratory receptors was noted involving either carotid chemoreceptors (n = 7) or pulmonary C-fibers (n = 4) or from both carotid chemoreceptors and pulmonary C-fibers (n = 6). In contrast, only one cardioreceptive NTS neuron was activated by distension of the carotid sinus. Recording sites recovered were confined to the medial NTS at the level of the area postrema and extended caudally into the commissural subnucleus. Our results indicate a convergence of carotid chemoreceptor and pulmonary C-fiber afferent inputs to cardioreceptive NTS neurons. With the paucity of baroreceptor inputs to these neurons it is suggested that sensory integration within the NTS may reflect regulatory versus defensive or protective reflex control.
Developing an Artificial Intelligence (A.I)‐based descriptor of facial appearance that fits with the assessments of makeup experts (2021)

Such automatic descriptor system seems providing a valuable help in making-up procedures and may extend to other activities such as Skincare or Haircare. As such it should allow large investigations to better evaluate the consumers' needs of esthetical improvements.
Improving the Brightness and Daylight Contrast of Organic Light‐Emitting Diodes (2005)

Abstract For most applications of displays based on organic light‐emitting diodes (LEDs), it is desirable to have good daylight contrast in combination with a high intensity of emitted light. The conventional approaches to enhance the daylight contrast, using a black cathode or circular polarizers, result in a significant loss of light emitted by the LED. A rather novel approach to enhance daylight contrast while keeping loss of emitted light to a minimum is the introduction of a chiral‐nematic film in the device. This approach leads to an increase in light efficiency by a factor of 1.8 (with respect to circular polarizers) with some loss in daylight contrast values within the reflection band of the chiral‐nematic film. Outside the reflection band, however, the contrast approaches infinity.
The Effects of a Soft Tissue Mimicking Medium and Increased Power Settings on the Location and Magnitude of Lithotripter Peak Positive Pressure (1993)

No AccessJournal of Urology1 Feb 1993The Effects of a Soft Tissue Mimicking Medium and Increased Power Settings on the Location and Magnitude of Lithotripter Peak Positive Pressure William J. Davros, Brian S. Garra, John J. Pahira, and Robert K. Zeman William J. DavrosWilliam J. Davros More articles by this author , Brian S. GarraBrian S. Garra More articles by this author , John J. PahiraJohn J. Pahira More articles by this author , and Robert K. ZemanRobert K. Zeman More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)36101-3AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail In vitro experiments showed that a tissue mimicking medium alters the peak positive pressure (p+), focal zone properties and frequency content of shockwaves compared with their behavior in water. The reduction in (p+) ranged from 5% at 10kV. to 19% at 18.1kV., when measured at the geometric focus with the tissue mimicking medium present. As power settings were increased, the relative gain in pressure was damped by attenuation. A 2mm. shift in the acoustic focus was seen both axially and laterally with the tissue mimicking medium. While the former is probably not significant, the latter may be clinically significant given the narrow lateral beam width at the acoustic focus. These attenuation experiments suggest that clinical targeting through tissue may not be as precise or result in as high peak pressures as the clinician expects. Especially at high power settings, the depth of tissue traversed should be minimized to limit attenuation effects. © 1993 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 149Issue 2February 1993Page: 390-394 Advertisement Copyright & Permissions© 1993 by The American Urological Association Education and Research, Inc.Keywordsshockwave-ultrasoniclithotripsypressureMetricsAuthor Information William J. Davros More articles by this author Brian S. Garra More articles by this author John J. Pahira More articles by this author Robert K. Zeman More articles by this author Expand All Advertisement PDF DownloadLoading ...
Fast DC-Type Electric Vehicle Charger Based On A Quasi-Direct Boost - Buck Rectifier (2019)

This work presents a DC-type fast electric vehicle battery charger featuring low switching losses. Herein, a conventional three-phase two-level voltage source rectifier with low capacitive energy storage operates with a discontinuous PWM modulation where each phase-legs stops switching for 240° of the grid fundamental period, i.e. only a single phase-leg switch every 60°. In this case, this AC-DC converter loses voltage controllability of the DC-link and thus a buck-type DC-DC converter is cascaded in order to provide the necessary voltage regulation and current limitation for the charging process of the electric vehicle. The presented circuit is benchmarked against other solutions for a designed 50 kW power capability battery charger when considering the charging of a 30 kWh Nissan Leaf. The results show a superior power efficiency of the studied system.
Figure S4 from Human CD4<sup>+</sup> T Cells Specific for Merkel Cell Polyomavirus Localize to Merkel Cell Carcinomas and Target a Required Oncogenic Domain (2023)

&lt;p&gt;CD4 T cells recognize the MCPyV epitope 'MDL' (CT-1-13).&lt;/p&gt;
Development and persistence of local and systemic antibody responses in adults given live attenuated or inactivated influenza A virus vaccine (1986)

An enzyme-linked immunosorbent assay was used to measure nasal-wash and serum isotype-specific hemagglutinin antibody responses in 109 seronegative (hemagglutination-inhibiting titer less than or equal to 1:8) adults vaccinated intranasally with live attenuated A/Washington/897/80 (H3N2) or A/California/10/78 (H1N1) cold-adapted (ca) virus or with licensed subvirion vaccine subcutaneously. Live and inactivated virus elicited serum immunoglobulin A (IgA) responses in 83 and 96% of vaccinees, respectively, and elicited serum IgG responses in 72 and 100% of vaccinees. Inactivated virus induced higher titers of serum antibodies than did live virus and stimulated a nasal-wash IgG response more often than did live virus (94 versus 59%, P less than 0.01). In contrast, only 38% of inactivated virus vaccinees had local IgA responses compared with 83% of live virus vaccinees. Serum IgA and IgG and nasal IgG antibody titers remained elevated above prevaccination levels for at least 6 months in most of the live and inactivated vaccine responders, but the mean level of local IgA antibody induced by infection with live virus vaccine, in particular, decreased substantially. Considered in the context of previous work, the finding that live virus vaccine induced relatively long-lasting antibody in both local and serum compartments suggested that this vaccine may be a suitable alternative to inactivated vaccine for use in healthy persons.
A prospective observational study with dose volume parameters predicting rectosigmoidoscopic findings and late rectosigmoid bleeding in patients with uterine cervical cancer treated by definitive radiotherapy (2013)

D5cc may be a more reliable estimate than other dose-volumetric parameters for predicting the risk of RMC ≥ score 3 and LRC ≥ grade 2 in CT-based brachytherapy.
Behavior of Liquid Crystals in Electric and Magnetic Fields (1998)

The prelims comprise: Introduction Direct Influence of an Electric or Magnetic Field on Liquid Crystal Structure Distortions due to Direct Interaction of a Field with the Director Electrohydrodynamic Instabilities Conclusion References
Mapping and Ablation of Left Atrial Flutters (2000)

LA reentrant tachycardias are related to individually varying circuits and are amenable to mapping guided radiofrequency ablation.
Compact dual-polarized VHF microstrip antenna for satellite applications (2012)

This paper presents the design of a compact dual-polarized VHF microstrip antenna for modern small satellites. It consists of a slot-loaded annular-ring microstrip antenna printed on a light substrate to reduce its mass. A simple matching circuit is proposed to match the high input impedance of this antenna while enabling the generation of a dual linear-polarization. The size of the proposed design is finally equal to 450 mm at 161 MHz, i.e. λ <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0</sub> =4.
OSPFv3 Extensions for BIER (2018)

Bit Index Explicit Replication (BIER) is an architecture that provides
multicast forwarding through a without requiring
intermediate routers to maintain multicast related per-flow state.
Neither does BIER require an explicit tree-building protocol for its
operation. A multicast data packet enters a BIER domain at a Bit-
Forwarding Ingress Router (BFIR), and leaves the BIER domain at one
or more Bit-Forwarding Egress Routers (BFERs). The BFIR router adds
a BIER header to the packet. Such header contains a bit-string in
which each bit represents exactly one BFER to forward the packet to.
The set of BFERs to which the multicast packet needs to be forwarded
is expressed by the according set of bits set in BIER packet header.
This document describes the OSPFv3 [RFC8362] protocol extensions
required for BIER with MPLS encapsulation [RFC8296]. Support for other
encapsulation types is outside the scope of this document. The use of
multiple encapsulation types is outside the scope of this document.
COOLING IN A COMPOUND BUCKET (2015)

Electron cooling in the Fermilab Recycler ring is found to create correlation between longitudinal and transverse tails of the antiproton distribution. By separating the core of the beam from the tail and cooling the tail using “gated ” stochastic cooling while applying electron cooling on the entire beam, one may be able to significantly increase the over all cooling rate. In this paper, we describe the procedure and first experimental results.
Novel algorithm for coherence level measurement using R-R interval of ECG signal (2016)

In the field of biomedical engineering analysis of (Electrocardiogram) ECG signal is the most studied topic. In ECG signal the RR interval is distinguishable for different physiological state and for each state the coherence level in heart rhythms also different. In this paper a new approach for coherence estimation during different emotional state is proposed. The RR interval from patient ECG signal is calculated and then coherence level is calculated using walch algorithm. In this paper detailed process for R peak calculation as well coherence estimation is discussed. Estimated coherence finds application mainly in heart rate variability investigations and in neurology as well.
ChemInform Abstract: Reduction of α‐Amino Nitriles with Sodium. (1990)

Abstract The unsaturated α‐amino nitrile (I) is decyanated upon treatment with sodium in liquid ammonia to give the amine (II).
Dropping Users in a Multi-Antenna Broadcast Channel (2006)

Abstract- We analyze the sensitivity of the capacity of a multi-antenna multi-user system to the number of users being served simultaneously. We show analytically that the capacity loss of serving fewer users at low SNR is much smaller than at high SNR. The advantages of serving fewer users are many: multi-user algorithms often have better performance, lower complexity, and reduced channel-knowledge requirements. We provide guidelines on how many users to serve to get nearcapacity performance with low complexity. For example, we show that the performance gain in some algorithms more than compensates for the loss in capacity in an eight-antenna eight-user system when only four users are served, and we are now approximately 2dB from capacity at very low SNR. I.
Interleukin 1 stimulates granulocyte macrophage colony-stimulating activity release by vascular endothelial cells. (1986)

Studies designed to characterize monocyte-derived recruiting activity (MRA) a monokine that stimulates endothelial cells to produce granulocyte macrophage-colony-stimulating activity (CSA) by endothelial cells, show that it is a thermolabile protein of from 12,000 to 24,000 D which, on chromatofocusing, shows three separate peaks of eluted activity from pH 7.5 to 5.0. Because these and many other properties of MRA are identical to those of interleukin 1 (IL-1), we tested the hypothesis that MRA and IL-1 are identical. We cultured vascular endothelial cells with various concentrations of purified native and recombinant IL-1 (pI 7 form), then tested the endothelial cell supernatants for GM-CSA. Purified native IL-1 and recombinant IL-1 stimulated endothelial cells to release CSA. The MRA of native IL-1, recombinant IL-1, and unfractionated monocyte conditioned medium was neutralized by a highly specific rabbit anti-human IL-1 antiserum. Chromatofocusing fractions that contained MRA contained immunoreactive IL-1 on immunoblotting and the bioactivity was neutralized completely by treatment with the antiserum. We conclude that IL-1 induces the release of CSA by vascular endothelial cells, that IL-1 is constitutively produced by monocytes in vitro, and that MRA and IL-1 are biologically, biophysically and, immunologically identical.
WhammyPhone (2016)

We present WhammyPhone, a novel audio interface that supports physical manipulation of digital audio through bend gestures. WhammyPhone combines a high-resolution flexible display, bend sensors, and a set of intuitive interaction techniques that enable novice users to manipulate sound in a tangible fashion. With WhammyPhone, bend gestures can control both discrete (e.g. triggering a note) and continuous parameters (e.g. pitch bend). We showcase application scenarios that leverage the unique input modalities of WhammyPhone and discuss its potential for digital audio manipulation.
Mullite for Structural, Electronic, and Optical Applications (1991)

Mullite (3Al 2 O 3 ·2SiO 2 ) is becoming increasingly important in electronic, optical, and high‐temperature structural applications. This paper reviews the current state of mullite‐related research at a fundamental level, within the framework of phase equilibria, crystal structure, synthesis, processing, and properties. Phase equilibria are discussed in terms of the problems associated with the nucleation kinetics of mullite and the large variations observed in the solid‐solution range. The incongruent melting behavior of mullite is now widely accepted. Large variations in the solid solubility from 58 to 76 mol% alumina are related to the ordering/disordering of oxygen vacancies and are strongly coupled with the method of synthesis used to form mullite. Similarly, reaction sequences which lead to the formation of mullite upon heating depend on the spatial scale at which the components are mixed. Mixing at the atomic level is useful for low‐temperature (&lt;1000°C) synthesis of mullite but not for low‐temperature sintering. In contrast, precursors that are segregated are better suited for low‐temperature (1250° to 1500°C) densification through viscous deformation. Flexural strength and creep resistance at elevated temperatures are significantly affected by the presence of glassy boundary inclusions; in the absence of glassy inclusions, polycrystalline mullite retains &gt;90% of its room‐temperature strength to 1500°C and displays very high creep resistance. Because of its low dielectric constant, mullite has now emerged as a substrate material in high‐performance packaging applications. Interest in optical applications mainly centers on its applicability as a window material within the mid‐infrared range.
Voltage-controlled accommodating IOL system using an ion polymer metal composite actuator (2016)

Surgeons treat cataracts by replacing the clouded lens with an intraocular lens (IOL), but patients are required to wear reading glasses for tasks requiring near vision. We suggest a new voltage-controlled accommodating IOL made of an ionic polymer metal composite (IPMC) actuator to change focus. An in vitro experiment was conducted where an actuator was placed inside the eye and moved with applied voltage. The lens attached to the actuator was deformed by its movement to change the patient's focus. The results showed that this system can accommodate a change of approximately 0.8 diopters under an applied voltage of ± 1.3 V.