gebhart/patrepeval huggingface dataset:
Positron emission tomography reporter gene strategy for use in the central nervous system (2019)

There is a growing need for monitoring or imaging gene therapy in the central nervous system (CNS). This can be achieved with a positron emission tomography (PET) reporter gene strategy. Here we report the development of a PET reporter gene system using the PKM2 gene with its associated radiotracer [<sup>18</sup>F]DASA-23. The PKM2 reporter gene was delivered to the brains of mice by adeno-associated virus (AAV9) via stereotactic injection. Serial PET imaging was carried out over 8 wk to assess PKM2 expression. After 8 wk, the brains were excised for further mRNA and protein analysis. PET imaging at 8 wk post-AAV delivery showed an increase in [<sup>18</sup>F]DASA-23 brain uptake in the transduced site of mice injected with the AAV mice over all controls. We believe PKM2 shows great promise as a PET reporter gene and to date is the only example that can be used in all areas of the CNS without breaking the blood-brain barrier, to monitor gene and cell therapy.
Figure S3 from Growth of Triple-Negative Breast Cancer Cells Relies upon Coordinate Autocrine Expression of the Proinflammatory Cytokines IL-6 and IL-8 (2023)

&lt;p&gt;Figure S3 - PDF file 1318K, Expression LPAR2 in breast cancers, and the importance of LPAR2 in the expression of cytokines in SUM159&lt;/p&gt;
The technology of CO2 sequestration by mineral carbonation: current status and future prospects (2017)

Mineral carbonation (MC) has been extensively researched all over the world since it was found as a naturally exothermic process to permanently sequester CO2. In order to accelerate the natural process, various methods for carbonation of Mg-/Ca- silicate minerals have been studied. It has been found that the MC efficiency will increase with an increase in CO2 pressure, retention time, temperature, mass ratio of Mg/Ca to Si in minerals, specific surface area, and the slurry concentration in a specific range, and with the introduction of NaCl and NaHCO3 or carbonic anhydrase. However, there is still no successful industrial application because of high economic costs and slow reaction rate. It is not economic to exploit Mg-/Ca- silicate minerals deposits or tailings to sequester CO2 by the MC due to the cost of grinding and heat pre-treatment. In some cases, the whole sequestration process may result in more CO2 emissions than the sequestered CO2 due to the requirements of energy inputs. The process, however, may be profitable as a whole (with carbon credits). It is suggested to combine the MC with valuable metals recovery from ore deposits in order to reduce the cost of the MC by cost sharing for mineral recovery.
Closed-Form Non-Singular Constant-Curvature Continuum Manipulator Kinematics (2020)

Continuum manipulators describe robotic arms which achieve motion by bending continuously along their length. This paper presents a kinematic parameterization of such arms, which avoids a problematic singularity common to previous approaches. It provides a closed-form and computationally efficient description of the kinematics of joint sections that are assumed to make circular arcs. This approach applies to a broad class of manipulator designs and does not depend on the number or position of actuators (e.g. tendons or artificial muscles). This parameterization has enabled real-time dynamics modeling of robotic manipulators composed of continuum joints.
Typing on Tap: Estimating a Finger-Worn One-Handed Chording Keyboard’s Text Entry Rate (2021)

The Tap StrapTM enables eyes-free mobile typing using a one-hand device worn on the user’s fingers. Using the standard MacKenzie-Soukoreff text entry phrase set, 12 participants completed 480 minutes of practice with the keyboard on either their dominant or non-dominant hand. Average final typing rate was 22.11 words per minute (WPM), and letter accuracy increased from 85.11% to 91.02%. Non-dominant hand users typed more accurately than dominant after 420 minutes of practice.
COBRA-TF Simulation of Fuel Thermal Response During Reactivity Initiated Accidents Using the NSRR Pulse Irradiation Experiments (2014)

COBRA-TF (Coolant Boiling in Rod Arrays – Two Fluid) or CTF is a transient subchannel code, selected to be the reactor core thermal hydraulic (T/H) simulation tool in the multi-physics code development project of the Consortium for Advanced Simulation of Light Water Reactor (CASL) sponsored by the US Department of Energy. CTF is currently being evaluated and further improved by CASL as part of its multi-physics software package to help the nuclear industry address operational and safety challenge problems, such as Departure from Nucleate Boiling (DNB) and Reactivity Initiated Accidents (RIA). In this paper, CTF’s capability for transient fuel thermal analysis, including DNB prediction is evaluated by modeling and simulating power burst experiments with high burnup PWR fuel rods, conducted at the Nuclear Safety Research Reactor (NSRR) in Japan. The experiments were a series of tests performed using pulse irradiation capability of the reactor to evaluate fuel rod failure with respect to fuel enthalpy, coolant conditions, and fuel design during RIAs such as control rod ejection. Specific to this study, the experiments using the Takahama-3 reactor fuel segments have been modeled and simulated to evaluate CTF’s prediction capability for DNB onset, fuel rod thermal response, and heat transfer from single-phase to post-CHF during fast RIA transients.
Multi Media Enhancement Of The Electrical Engineering Core Course (2020)

The objective of this paper is to present a new multi-media teaching enhancement tools for the principles of electrical engineering core course at the University of Central Florida. The new teaching environment will enhance student learning by providing innovative methods for interactive teaching and testing. Students will be able to carry on simulation on the web, virtually at any place. To demonstrate the capabilities of the web-based software, we will give the detailed description for possible ac and dc simulation on line in order to illustrate the proposed concept. Once fully implemented, the new web-based simulation tools will enhance electrical engineering learning of the basic concepts, improve design capabilities, and provide the students with the opportunity for selfevaluation and testing. In the final analysis, the web-site will provide Graphical User Interface (GUI) capabilities that reside in the client desktop, allowing students to be able to perform on-line simulation of all type of DC and AC electrical engineering problems and have the option of plotting the responses graphically. This paper gives only the first development phase of the project. More works is needed to complete the software development of the simulation component of the Circuit Mark-Up Language (CirML) module.
A Highly Stable Adamantyl-Substituted Pincer-Ligated Iridium Catalyst for Alkane Dehydrogenation (2010)

The adamantyl-substituted pincer-ligand precursor AdPCP-H [(AdPCP = κ3-C6H3-2,6-(CH2PAd2)2); Ad = 1-adamantyl] has been synthesized by the reaction of 1,3-dibromoxylene with di-1-adamantylphosphine in the presence of triethylamine. Treatment of AdPCP-H with [Ir(COD)Cl]2 (COD = 1,5-cyclooctadiene) affords the pincer-ligated complex (AdPCP)IrHCl, which was crystallographically characterized. Dehydrohalogenation of (AdPCP)IrHCl either with LiBEt3H or with KOtBu, under hydrogen atmosphere, yields the hydrides (AdPCP)IrH2 and (AdPCP)IrH4. (AdPCP)IrH2 catalyzes dehydrogenation of alkanes with a level of activity comparable to that of the previously reported (tBuPCP)IrH2, while it is thermally much more robust than the tBuPCP analogue, as well as iPrPCP or tBuPOCOP pincer complexes.
2015 (2015)

Acetate templating for total hip arthroplasty (THA) is cumbersome and inaccurate. High cost hampers mainstream use of digital templating despite ease of use and accuracy. The aim of this study was to validate a low-cost digital THA templating system. Low-cost digital templating software was created using C# programming language. On the basis of power calculations, three surgeons templated 20 consecutive anteroposterior pelvis X-rays using this software against an industry standard. Intraclass correlation coefficient for both systems was approximately .90 for component size and femoral neck cut position. Bland-Altman plots demonstrated that both systems predicted actual implant size with similar accuracy. Interrater reliability was not significantly different between the two systems. This low-cost digital THA templating system is up to 12-fold lower in cost than currently available software with similar accuracy.
Photoluminescence Response of Terbium-Exchanged MFI-Type Materials to Si/Al Ratio, Texture, and Hydration State (2006)

Terbium-exchanged MFI zeolite type materials, i.e., microporous-mesoporous Zeotile-1 with the Si/Al ratio in the range 33-200, Zeogrid with the Si/Al ratio of 75, and nanocrystalline MFI with the Si/Al ratio of 75, were prepared via an ion exchange procedure. All of these zeolites were investigated by means of time-resolved photoluminescence techniques in various hydration states: as-synthesized (hydrated), calcined (heated at 450 degrees C in air), and rehydrated (after a six-month exposure to the atmospheric moisture). The photoluminescence decays and spectra were analyzed by discrete exponential fitting, distribution lifetimes analysis, and area-normalized time-resolved photoluminescence spectra. The results sustained a single average terbium species coordinated to both water molecules and framework oxygens in the hydrated zeolites. The framework contribution increased with the Si/Al ratio in Zeotile-1 and was greatest for the nanocrystalline MFI zeolite. For the calcined Zeotile-1 and Zeogrid, two main terbium species of different environments were found. For the nanocrystalline Tb3+-MFI, a distinct number of species could not be inferred, indicating a more heterogeneous distribution. Rehydration further differentiated among the Tb3+-exchanged zeolites. Photoluminescence line shape and decay of Tb3+-Zeotile-1 were between those of the hydrated and calcined states indicating a slow rehydration rate in contrast with the photoluminescence properties of Tb3+-MFI, which fully recovered the values of the hydrated state. Tb3+-Zeogrid presented an intermediate case: while the PL line shape was fully restored to that measured for the hydrated sample, the decay was still longer than that measured with the hydrated sample. Terbium photoluminescence response related to zeolite texture, Si/Al ratio, and hydration state suggest different sitting and location of terbium in Zeotile-1, Zeogrid, and nanocrystalline MFI materials. In mesoporous Zeotile-1 and Zeogrid, the results sustained two types of terbium sites: one on the internal surface of mesopores, the other inside the pores, while for the nanocrystalline MFI, terbium sites inside the pores predominate.
The vagal ganglia transcriptome identifies candidate therapeutics for airway hyperreactivity (2018)

Mainstay therapeutics are ineffective in some people with asthma, suggesting a need for additional agents. In the current study, we used vagal ganglia transcriptome profiling and connectivity mapping to identify compounds beneficial for alleviating airway hyperreactivity (AHR). As a comparison, we also used previously published transcriptome data from sensitized mouse lungs and human asthmatic endobronchial biopsies. All transcriptomes revealed agents beneficial for mitigating AHR; however, only the vagal ganglia transcriptome identified agents used clinically to treat asthma (flunisolide, isoetarine). We also tested one compound identified by vagal ganglia transcriptome profiling that had not previously been linked to asthma and found that it had bronchodilator effects in both mouse and pig airways. These data suggest that transcriptome profiling of the vagal ganglia might be a novel strategy to identify potential asthma therapeutics.
Oxidative burst and NO generation as initial response to ischemia in flow-adapted endothelial cells (2001)

Shear stress modulates endothelial physiology, yet the effect(s) of flow cessation is poorly understood. The initial metabolic responses of flow-adapted bovine pulmonary artery endothelial cells to the abrupt cessation of flow (simulated ischemia) was evaluated using a perfusion chamber designed for continuous spectroscopy. Plasma membrane potential, production of reactive O 2 species (ROS), and intracellular Ca 2+ and nitric oxide (NO) levels were measured with fluorescent probes. Within 15 s after flow cessation, flow-adapted cells, but not cells cultured under static conditions, showed plasma membrane depolarization and an oxidative burst with generation of ROS that was inhibited by diphenyleneiodonium. EGTA-inhibitable elevation of intracellular Ca 2+ and NO were observed at ∼30 and 60 s after flow cessation, respectively. NO generation was decreased in the presence of inhibitors of NO synthase and calmodulin. Thus flow-adapted endothelial cells sense the altered hemodynamics associated with flow cessation and respond by plasma membrane depolarization, activation of NADPH oxidase, Ca 2+ influx, and activation of Ca 2+ /calmodulin-dependent NO synthase. This signaling response is unrelated to cellular anoxia.
Exhaustive search of the configurational space of heat‐shock protein 90 with its inhibitor by multicanonical molecular dynamics based dynamic docking (2020)

Multicanonical molecular dynamics based dynamic docking was used to exhaustively search the configurational space of an inhibitor binding to the N-terminal domain of heat-shock protein 90 (Hsp90). The obtained structures at 300 K cover a wide structural ensemble, with the top two clusters ranked by their free energy coinciding with the native binding site. The representative structure of the most stable cluster reproduced the experimental binding configuration, but an interesting conformational change in Hsp90 could be observed. The combined effects of solvation and ligand binding shift the equilibrium from a preferred loop-in conformation in the unbound state to an α-helical one in the bound state for the flexible lid region of Hsp90. Thus, our dynamic docking method is effective at predicting the native binding site while exhaustively sampling a wide configurational space, modulating the protein structure upon binding.
A 93.7% Efficient 400A 48V-1V Merged-Two-Stage Hybrid Switched-Capacitor Converter with 24V Virtual Intermediate Bus and Coupled Inductors (2021)

This paper presents a 93.7% efficient 400 A 48 V-1 V merged-two-stage hybrid switched-capacitor converter with 24 V virtual intermediate bus and coupled inductors. The first stage of the converter is a 2:1 interleaved charge pump which converts the 48 V input voltage to a virtual intermediate bus at 24 V. The second stage contains multiple paralleled 24:1 four-phase series capacitor buck modules with coupled inductors which have an equivalent voltage conversion ratio of 6:1. The two stages are linked by a 24 V virtual intermediate bus with small decoupling capacitor and voltage ripple. The hybrid switched capacitor architecture enables high efficiency, high power density, and high control bandwidth for ultra-high-current point-of-load applications. The effectiveness of the topology is verified by a 48 V-1 V 400 A prototype with a peak efficiency of 93.7% at 120 A and a full load efficiency of 88.7% at 400 A. The power density of the prototype is 342 W/in <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sup> . Compared to a decoupled-two-stage design, the merged-two-stage design reduces the passive component size at the cost of slightly reduced efficiency.
Structural and Mechanistic Analysis of Drosophila melanogaster Agmatine N-Acetyltransferase, an Enzyme that Catalyzes the Formation of N-Acetylagmatine (2017)

Agmatine N-acetyltransferase (AgmNAT) catalyzes the formation of N-acetylagmatine from acetyl-CoA and agmatine. Herein, we provide evidence that Drosophila melanogaster AgmNAT (CG15766) catalyzes the formation of N-acetylagmatine using an ordered sequential mechanism; acetyl-CoA binds prior to agmatine to generate an AgmNAT•acetyl-CoA•agmatine ternary complex prior to catalysis. Additionally, we solved a crystal structure for the apo form of AgmNAT with an atomic resolution of 2.3 Å, which points towards specific amino acids that may function in catalysis or active site formation. Using the crystal structure, primary sequence alignment, pH-activity profiles, and site-directed mutagenesis, we evaluated a series of active site amino acids in order to assign their functional roles in AgmNAT. More specifically, pH-activity profiles identified at least one catalytically important, ionizable group with an apparent pK<sub>a</sub> of ~7.5, which corresponds to the general base in catalysis, Glu-34. Moreover, these data led to a proposed chemical mechanism, which is consistent with the structure and our biochemical analysis of AgmNAT.
Sensory, biochemical and microbiological changes of farmed catfish (Clarias batrachus, Linnaeus) and red tilapia (Oreochromis sp.) at Ambient Storage (1995)

Farmed catfish (Clarias batrachus, Linnaeus) and red tilapia (Oreochromis sp.) were stored at ambient temperature (28±2°C) for a period of 24 h. They were evaluated for acceptability, freshness deterioration, rancidity development and microbiological changes. Raw catfish was acceptable up to 20 h and that of tilapia up to 15 h. Upon rejection their KJ values were 70.0 and 42.7% for tilapia and catfish respectively. Microbiologically, catfish samples did not exceed the 107 cfu/g limit until the end of the storage period; however, tilapia was not acceptable by the 20th hour.
ChemInform Abstract: Synthesis of a Triphenylphosphine Reagent on Non‐Cross‐Linked Polystyrene Support: Application to the Staudinger/Aza‐Wittig Reaction. (2001)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Rupture of Papillary Muscle and Chordae Tendinae Complicating STEMI: A Call for Action (2020)

Papillary muscle rupture (PMR) or chordae tendinae rupture (CTR) is a rare but lethal complication after ST elevation myocardial infarction (STEMI). Due to the rarity of this condition, there are limited studies defining its epidemiology and outcomes. This is a retrospective study from Nationwide Inpatient Sample database from 2002 to 2014 of patients with STEMI and PMR/CTR. Outcomes of interest were incidence of in-hospital mortality, cardiogenic shock (CS), utilization of mechanical circulatory support (MCS) devices and mitral valve procedures (MVPs) among patients with and without rupture. We also performed simulation using the cardiovascular model to better understand the hemodynamics of severe mitral regurgitation and effects of different medications and device therapy. We identified 1,888 patients with STEMI complicated with PMR/CTR. Most of the patients were >65 years of age (65.3%), male (63.6%), and white (82.3%). They had significantly higher incidence of CS, cardiac arrest, and utilization of MCS devices. In-hospital mortality was higher in patients with rupture (41% vs. 7.40%, p < 0.001) which remained unchanged over the study period. Hospitalization cost and length of stay was also higher in them. MVP and revascularization led to better survival rates (27.9% vs. 60.6%, adjusted OR: 0.14; 95% CI: 0.10-0.19; p < 0.001). Despite significant advancement in the revascularization strategy, PMR/CTR after STEMI continues to portend poor prognosis with high inpatient mortality. Cardiogenic shock is a common presentation and is associated with significantly inpatient mortality. Future studies are needed determine the best strategies to improve outcomes in patients with STEMI with PMR/CTR and CS.
Defects in electron-irradiated InP studied by positron lifetime spectroscopy (1997)

A study of electron-irradiation-induced defects in undoped InP was carried out. The irradiation was performed at low temperature (4 K) with an incident energy of 2 MeV. The defect formation and annealing behavior in dependence on the fluence (${10}^{15}$--${10}^{19}$ ${\mathrm{cm}}^{\mathrm{\ensuremath{-}}2}$) were studied in InP. Annealing experiments between 90 and 800 K and temperature-dependent measurements between 20 and 600 K were performed. A main annealing stage between 140 and 210 K dependent on the irradiation fluence was attributed to the annealing of Frenkel pairs in both sublattices. After an annealing temperature of about 350 K a second vacancylike defect becomes detectable by positrons. This defect contains several vacancies and their number increases with irradiation fluence. The annealing stage for these clusters occurs between 590 and 800 K dependent on the cluster size.
A novel synthesis of α‐N‐methyl‐14C‐DL‐asparagine by generation of methylamine‐14C insitu (1981)

Abstract A method is described by which methylamine‐ 14 C is qenerated from its hydrochloride by N,N‐diisopropylethylamine in the presence of maleamic acid to produce the title compound in high yield.
Search for heavy Majorana neutrinos in μ±μ± + jets events in proton–proton collisions at s=8 TeV (2015)

A search is performed for heavy Majorana neutrinos (N) using an event signature defined by two muons of the same charge and two jets (μ±μ±jj). The data correspond to an integrated luminosity of 19.7fb^(−1) of proton–proton collisions at a center-of-mass energy of 8TeV, collected with the CMS detector at the CERN LHC. No excess of events is observed beyond the expected standard model background and upper limits are set on |V_(μN)|^2 as a function of Majorana neutrino mass m_N for masses in the range of 40–500GeV, where V_(μN) is the mixing element of the heavy neutrino with the standard model muon neutrino. The limits obtained are |V_(μN)|^2&lt;0.00470 for m_N=90GeV, |V_(μN)|^2&lt;0.0123 for m_N=200GeV, and |V_(μN)|^2&lt;0.583 for m_N=500GeV. These results extend considerably the regions excluded by previous direct searches.
The predictive power of the microbiome exceeds that of genome-wide association studies in the discrimination of complex human disease (2020)

Abstract Over the past decade, studies of the human genome and microbiome have deepened our understanding of the connections between human genes, environments, microbes, and disease. For example, the sheer number of indicators of the microbiome and human genetic common variants associated with disease has been immense, but clinical utility has been elusive. Here, we compared the predictive capabilities of the human microbiome versus human genomic common variants across 13 common diseases. We concluded that microbiomic indicators outperform human genetics in predicting host phenotype (overall Microbiome-Association-Study [MAS] area under the curve [AUC] = 0.79 [SE = 0.03], overall Genome-Wide-Association-Study [GWAS] AUC = 0.67 [SE = 0.02]). Our results, while preliminary and focused on a subset of the totality of disease, demonstrate the relative predictive ability of the microbiome, indicating that it may outperform human genetics in discriminating human disease cases and controls. They additionally motivate the need for population-level microbiome sequencing resources, akin to the UK Biobank, to further improve and reproduce metagenomic models of disease.