gebhart/patrepeval huggingface dataset:
Interaction of High Molecular Weight Kininogen with Endothelial Cell Receptors suPAR, gC1qR and Cytokeratin 1 by Surface Plasmon Resonance (BiaCore). (2005)

Abstract The physiologic activation of the plasma kallikrein kinin system (KKS) requires the assembly of these proteins on the cell membrane. High molecular weight kininogen (HK) binds to endothelial cells through an interaction with a multiprotein receptor complex that consist of: urokinase plasminogen activator receptor (uPAR), globular C1q receptor (gC1qR) and cytokeratin 1 (CK1). The affinity of HK and cleaved HK (Hka) for endothelial cells is KD=7–52 nM but the affinity for each of the three binding proteins is unknown. We first examined the direct binding of HK and Hka to the soluble receptor form of uPAR (suPAR), gC1qR and CK1 using surface plasmon resonance (BiaCore). We linked suPAR, gC1qR and CK1 (800–1100 pg/mm2) by amine coupling to a CM-5 chip and perfused HK and Hka at a concentration ranging from 50 to 400 nM in the presence or absence of 10 μM ZnCl2. A Langmuir binding model with local fit (stoichiometry of 1:1) was used to analyse kon (association rate constant), koff (dissociation rate constant) and KD (equilibrium dissociation constant) for HK and Hka. HK Hka KD kon koff KD kon koff Immobilized Protein nM M-1s-1 x104 s-1 x10-4 nM M-1s-1 x104 s-1 x10-4 gC1qR 0.8±0.7 12.3±5.0 0.8±0.5 0.7±0.5 24.4±10.9 1.4±0.5 CK1 15.1±1.0 3.6±0.3 5.4±0.7 6.3±1.9 5.8±0.3 3.6±0.9 suPAR 2313.5±1465.3 1.0±0.7 164.8±22.2 43.6±16.6 21.3±11.9 78.5±10.6 The binding of HK and Hka to the three receptors was zinc dependent. The affinity for HK and Hka was gC1qR&gt;CK1&gt;suPAR. The high affinity for gC1qR and CK1 was similar for HK and Hka and was due to a very slow koff. The affinity for Hka compared to HK was 2.5-fold tighter for CK1 but 50-fold tighter for suPAR. A reversed immobiization was then performed. We immobilized HK, Hka and LK (800 pg/mm2) and suPAR, gC1qR and CK1 were flowed over the chip at a concentration ranging from 50 to 700 nM. Only gC1qR bound to immobilized HK and Hka, with a KD of 6.31± 2.8 pM for HK and 2.86±1.5 pM for Hka while the other 2 receptors did not bind. Receptor integrity was shown by preincubating HK or Hka with the pure receptors and observing competition with the immobilized receptors. Among the HK/Hka receptors, complex formation was only observed between gC1qR and immobiized CK1 with or without Hka indicating that a CK1-gC1qR complex can form independently of kininogen. This study indicates that although suPAR has the weakest affinity of the three receptors it is the only one that distinguishes between HK and Hka. Only one binary complex was revealed by this technique between CK1 and gC1qR. These results lay the foundation for anlayzing the affinity of these receptors in a cellular environment.
Comparison of Routing Methods for DCS-Switched Networks (1993)

The market for nonswitched, high-rate digital leased lines (also called digital “private line” services) for applications such as data and video is lucrative and growing rapidly; the Bellcore client companies are experiencing acute competition in this area. We developed an operations and network architecture to enable them to compete effectively in this highly competitive market. Its dynamic routing allows fast connection times and more efficient use of network capacity than existing routing methods. A comparison of dynamic routing methods with existing hierarchical methods shows a reduction in network capacity of roughly 14 percent with dynamic routing. Bellcore used these results to support a business analysis for Bellcore client companies, who then chose this architecture as a strategic initiative for implementation in their networks.
Dynamic ZVS direct on-line start up of current fed resonant converter using initially forced DC current (2002)

ZVS (zero voltage switching) is critical for the reliable operation of normal current fed DC-AC resonant converters. In the steady state, ZVS can be achieved by many available techniques such as PLL, VCO and other integral controllers. However, during the transient process like start up, to achieve ZVS is not an easy task for these controllers and consequently switch failure may occur. Based on numerical and analytical analyses, this paper proposes a novel method using an initially forced DC current to achieve ZVS during start up. Start up conditions are discussed and complete dynamic ZVS is achieved. Considering the ramp up delay of the DC power supply, the start up can be controlled completely overshoot free at no extra cost. The validity of this method has been proven with PSpice simulations and experimental results.
Wave energy-assisted fluidic self-assembly of LED chips for display applications (2022)

Micro-light-emitting diode (micro-LED) displays have excellent image characteristics, particularly in terms of contrast ratio, response to electric field, and color expressions. However, these are expensive due to the price of the LED chips. This can be overcome by reducing the size of the LED chip, but the efficiency will decrease as a result. Moreover, the rapid and accurate arrangement of a few million chips with a size of ∼50 µm to form pixels on the substrate is a challenging task. In this study, fluidic self-assembly process was introduced. The self-assembly design and implementation were limited to building a micro-scale system. With geometric constraints, external forces may influence the outcome of a self-assembled product. In this case, wave energy was used as the external force to manipulate the LED chips on the substrate. Target-generated waveforms in the fluid were used to control the movement of the LED chips. The arrays of the LED chips were arranged on a fine metal mask, i.e. transfer cartridge. The chips were then transferred to a circuit-printed glass plate by face-to-face pressing under high temperature and high pressure. It was found that the wave energy-assisted self-assembly is applicable and beneficial to LED module fabrication.
Abstract P044: Urine Arsenic Concentrations and Glycated Hemoglobin: Associations by Diabetes Status in the Strong Heart Study (2012)

Introduction: Low-moderate exposure to inorganic arsenic has been associated with hemoglobin A1c (HbA1c) in some studies. While HbA1c is a marker of diabetes control among individuals with treated diabetes, the association between arsenic and HbA1c has not been evaluated according to diabetes status. Hypothesis: Arsenic is positively associated with HbA1c. Methods: We included 3,567 (1774 with diabetes and 1793 without diabetes) American Indian adults who participated in the Strong Heart Study in 1989-1991 with measured urine arsenic, HbA1c and other covariates. We compared HbA1c levels by urine arsenic quartiles using linear regression stratified by diabetes status and adjusted for urine creatinine, demographic variables, body mass index and smoking. Among diabetics, we ran models stratified by fasting glucose and by diabetes treatment. Results: Median (interquartile range) HbA1c levels were 8.1 (6.1, 10.3)% and 5.1 (4.7, 5.4)%, in participants with and without diabetes, respectively. Adjusted mean HbA1c levels were 0.55 (95% CI 0.20, 0.90)%, 1.13 (0.76, 1.49)% and 1.28 (0.77, 1.59)% higher in quartiles 2-4 compared to the lowest quartile; p for trend &lt;0.001. Urine arsenic was not associated with HbA1c levels in those without diabetes: mean difference in HbA1c levels comparing quartiles 2-4 to the lowest quartile were 0.08 (0.01, 0.15)%, -0.03 (-0.10, 0.05)% and -0.05 (-0.13, 0.04)%, respectively; p for trend =0.10. Among diabetics, the association between arsenic and HbA1c was strongest among participants with fasting glucose ≥180 mg/dL, in untreated participants, and in those taking only oral hypoglycemics (Table). Difference (95% Confidence Interval) in HbA1c by Total Urine Arsenic (adjusted for age, sex, region, obesity, smoking and urine creatinine) Fasting Plasma Glucose (mg/dL) Diabetes Treatment Urine Arsenic (μg/L) &lt;1.6 (N=291) ≥126 and &lt;180 (N=594) ≥180(N=865) None (N=688) Oral(N=637) Insulin (N=436) Q1 (&lt;8.83) 0.00 (referent) 0.00 (referent) 0.00 (referent) 0.00 (referent) 0.00 (referent) 0.00 (referent) Q2 (≥8.83 and &lt;15.38) 0.15 (-0.33,0.64) 0.04 (-0.34, 0.42) 0.41 (0.03, 0.80) 0.57 (0.07, 1.06) 0.37 (-0.15, 0.89) 0.35 (-0.27, 0.97) Q3 (≥15.38 and &lt;26.37) 0.43 (-0.12, 0.98) 0.38 (-0.04, 0.80) 0.49 (0.09, 0.90) 0.93 (0.39, 1.46) 0.73 (0.19, 1.28) 0.61 (-0.07, 1.28) Q4(≥26.37) −0.21 (-0.86, 0.44) 0.27 (-0.17, 0.71) 0.58 (0.12, 1.04) 0.91 (0.34, 1.49) 0.90 (0.25, 1.54) 0.36 (-0.38, 1.11) p for trend * 0.29 0.22 0.08 0.02 0.01 0.69 * Based on quartile medians Urine arsenic concentrations were positively associated with HbA1c in Strong Heart Study participants with diabetes. No association was found among participants without diabetes. Prospective analyses that evaluate the relationship between arsenic exposure and diabetes, including measures of diabetes control, are needed.
Indirect measures of visual cortex plasticity and GABA concentration are not correlated in adults with normal vision (2018)

Introduction Increases in visually-evoked potential (VEP) amplitude can be induced by rapid visual stimulation. This effect involves LTP mechanisms and provides an acute, objective measure of human visual cortex plasticity. Gamma-aminobutyric acid (GABA) may influence adult visual cortex plasticity. GABA concentration is inversely associated with alternation rate (AR) during binocular rivalry. Therefore, AR provides an indirect measure of GABA levels. Our aim was to test for an association between LTP and AR in young (18-40 years, n = 29) and older adults (60-80 years, n = 18). Two age groups were tested because GABA concentration changes across the lifespan. Methods Binocular rivalry was induced using orthogonal gratings viewed through anaglyphic glasses and ARs were averaged across 6 trials. LTP was assessed by comparing VEP amplitude for an onset/offset checkerboard stimulus (0.3 degree checksize, 4 degrees of visual angle) before and after 2 minutes of viewing the checkerboard presented at 9Hz. Pre and post VEPs were recorded for 3 minutes at a baseline frequency of 1Hz. Results ARs were significantly slower in older adults than young adults (t48=6.043, p< 0.001). LTP induction differed significantly between groups (F1=20.772, p< 0.001). VEP depotentiation (LTD) occurred in the young adult group (t28=3.444, p=0.002) vs. potentiation (LTP) for the older adult group (t17=-2.927, p=0.009). No correlation between AR and the change in VEP amplitude was observed for either group (p>0.05). Conclusion AR was not associated with VEP amplitude change following LTP induction. However, we did observe two unexpected effects. 1) Contrary to previous reports, visual cortex LTP could not be induced in younger adults, 2) greater LTP (higher visual cortex plasticity) occurred in the older group that had slower AR (higher visual cortex GABA concentration). Therefore, age substantially alters the response of the visual system to rivalrous stimuli and rapid visual stimulation. Meeting abstract presented at VSS 2018
Formation of Ethane Clathrate Hydrate in Ultrahigh Vacuum by Thermal Annealing (2022)

The existence of many molecules in the form of clathrate hydrates (CHs) in ultrahigh vacuum (UHV) and cryogenic conditions has not been explored adequately. In the present study, a detailed investigation by reflection absorption infrared spectroscopy confirmed that the three phases of ethane, i.e., amorphous, crystalline, and CH, coexist in a vapor-deposited ethane–water mixture at 60 K in UHV. Experiments were conducted with vapor-deposited ice films at 10 K, which were annealed to 60 K for tens of hours, and the IR spectral evolution was monitored systematically. Upon maintaining the system at 60 K, three phases of ethane were seen to coexist, but a gradual increase in the hydrate phase was noticed. The evolution of ethane CH from the amorphous ethane–water ice mixture was observed for the very first time in UHV under cryogenic conditions. The formation of the CH was further confirmed by temperature-programmed desorption (TPD) mass spectrometry. Quantum chemical calculation suggested the formation of 51262 cage of structure I CH in the ice matrix. The formation of ethane CH in a thin ice film at such a low temperature under UHV suggests its existence in the cometary environment.
Catalytic Arylhydroxylation of Dehydroalanine in Continuous Flow for Simple Access to Unnatural Amino Acids (2018)

This report discloses the first example of catalytic arylhydroxylation of dehydroalanine with aryldiazonium salts. Aryldiazonium salts, which are generated from aniline precursors under partially aqueous conditions in continuous flow, efficiently reacted with dehydroalanine in the presence of 10-15 mol % ferrocene to furnish α-hydroxyarylalanine derivatives (up to 82 % yield). The reactions proceeded with regioselectivity, broad functional group tolerance, and without polymerization of the dehydroalanine. Furthermore, the products were used to access α-unnatural amino acids, important targets with application in drug development.
Cytoskeleton Regulator PAK4 Plays a Role in Growth and Survival of Myeloma with a Potential Therapeutic Intervention Using PAK4 Allosteric Modulators (PAMs) (2014)

Abstract P21-activated serine/threonine kinase 4 (PAK4) is a major effector of Cdc42 and is essential for cytoskeleton reorganization. PAK4 is activated in cancer cells, promotes cell migration and anchorage-independent cell growth, and protects against apoptosis induction. With cellular migration playing a significant role in multiple myeloma (MM) cell growth and survival, we investigated the expression and subcellular localization of PAK4 in MM cells. We observed a high level of un-phosphorylated PAK4 in the cytoplasm and high levels of phosphorylated PAK4 in the nucleus. In a gain-of-function study, over-expression of PAK4-eGFP in PAK4-deficient MM cells (RPMI8226) significantly increased cell proliferation and survival. Conversely, in a loss-of-function study, conditional knock-down of PAK4 expression with TRIPZ-lentiviral vectors decreased MM cell proliferation and survival proportionally to the reduction in PAK4. With a significant impact of PAK4 on MM cell growth, we identified a class of orally bioavailable PAK4 allosteric modulators (PAMs; e.g. KPT-6604, -7189, -7657, -8752). We observed inhibition of MM cell growth and survival after treatment with PAMs even in the presence of bone marrow microenvironment. In addition, there is a significant correlation between PAK4 expression and the inhibition concentration (IC50s) of PAMs in proliferation assays. Moreover, inhibition of PAK4 induced receptor and mitochondrial-mediated apoptotic pathways via Caspase-3, -8, and -9 activation. PAMs had no significant effect on normal PBMCs, suggesting a favorable therapeutic index in MM treatment. Finally, in two murine models of human myeloma, orally bioavailable KPT-8752 given daily was able to inhibit tumor growth in vivo and prolong overall survival. In summary, PAK4 plays an important cellular and molecular function in myeloma and its inhibition with a new class of PAK4 allosteric modulators provides a novel therapeutic approach for the treatment of MM. Disclosures Senapedis: Karyopharm: Employment. Baloglu:Karyopharm: Employment. Anderson:Celgene: Consultancy; Sanofi-Aventis: Consultancy; Onyx: Consultancy; Acetylon: Scientific Founder, Scientific Founder Other; Oncoprep: Scientific Founder Other; Gilead Sciences: Consultancy.
Pho d 2, a major allergen from date palm pollen, is a profilin: cloning, sequencing, and immunoglobulin E cross‐reactivity with other profilins (2005)

Pho d 2, a 14.4 kDa protein identified as profilin, is a major and relevant allergen in DPP, as confirmed by SPT and thereby may elicit clinical symptoms in sensitized patients.
Observation of excited Ωc0 baryons in Ωb−→Ξc+K−π− decays (2021)

The first observation of the ${\mathrm{\ensuremath{\Omega}}}_{b}^{\ensuremath{-}}\ensuremath{\rightarrow}{\mathrm{\ensuremath{\Xi}}}_{c}^{+}{K}^{\ensuremath{-}}{\ensuremath{\pi}}^{\ensuremath{-}}$ decay is reported using $p$ $p$ collision data at center of mass energies of 7, 8, and 13 TeV collected by the LHCb experiment, corresponding to an integrated luminosity of $9\text{ }\text{ }{\mathrm{fb}}^{\ensuremath{-}1}$. Four excited ${\mathrm{\ensuremath{\Omega}}}_{c}^{0}$ baryons are observed in the ${\mathrm{\ensuremath{\Xi}}}_{c}^{+}$ ${K}^{\ensuremath{-}}$ mass projection of the ${\mathrm{\ensuremath{\Omega}}}_{b}^{\ensuremath{-}}\ensuremath{\rightarrow}{\mathrm{\ensuremath{\Xi}}}_{c}^{+}{K}^{\ensuremath{-}}{\ensuremath{\pi}}^{\ensuremath{-}}$ decays with the significance of each exceeding five standard deviations. They coincide with the states previously observed in prompt $pp$ and ${e}^{+}{e}^{\ensuremath{-}}$ production. Relative production rates, masses, and natural widths of the states are measured, and a test of spin hypotheses is performed. Moreover, the branching ratio of ${\mathrm{\ensuremath{\Omega}}}_{b}^{\ensuremath{-}}\ensuremath{\rightarrow}{\mathrm{\ensuremath{\Xi}}}_{c}^{+}{K}^{\ensuremath{-}}{\ensuremath{\pi}}^{\ensuremath{-}}$ is measured relative to the ${\mathrm{\ensuremath{\Omega}}}_{b}^{\ensuremath{-}}\ensuremath{\rightarrow}{\mathrm{\ensuremath{\Omega}}}_{c}^{0}{\ensuremath{\pi}}^{\ensuremath{-}}$ decay mode and a precise measurement of the ${\mathrm{\ensuremath{\Omega}}}_{b}^{\ensuremath{-}}$ mass of $6044.3\ifmmode\pm\else\textpm\fi{}1.2\ifmmode\pm\else\textpm\fi{}1.{1}_{\ensuremath{-}0.22}^{+0.19}\text{ }\text{ }\mathrm{MeV}$ is obtained.
Cathode Materials: Ni and Co Segregations on Selective Surface Facets and Rational Design of Layered Lithium Transition‐Metal Oxide Cathodes (Adv. Energy Mater. 9/2016) (2016)

In article number 1502455, Ji-Guang Zhang, Chong-Min Wang and co-workers show that Ni tends to segregate on the (200) facet and form spinel-like structures, while Co segregates predominantly on the (20-2) facet to form rock salt structures. The cover image depicts the distinctive crystallographic surface selective segregation of Ni and Co in lithium transition metal oxides as a cathode for rechargeable lithium ion batteries.
Effective and rapid sealing of coronary, aortic and atrial suture lines (2015)

PreveLeak prevented bleeding at 98.4% of treated sites and was well tolerated; adverse events were consistent with those commonly observed in subjects undergoing surgical procedures. These results compared favourably with published studies of other sealants. The observed prevention of bleeding is clinically important in cardiac surgical patients. A randomized, comparative study is justified to further evaluate PreveLeak and confirm the findings from this study.
Production of 4He and 4He‾ in Pb–Pb collisions at sNN=2.76TeV at the LHC (2017)

Results on the production of 4He and nuclei in Pb–Pb collisions at sNN=2.76TeV in the rapidity range |y|<1, using the ALICE detector, are presented in this paper. The rapidity densities corresponding to 0–10% central events are found to be dN/dyHe4=(0.8±0.4(stat)±0.3(syst))×10−6 and , respectively. This is in agreement with the statistical thermal model expectation assuming the same chemical freeze-out temperature (Tchem=156MeV) as for light hadrons. The measured ratio of is 1.4±0.8(stat)±0.5(syst).
Modeling User Consumption Sequences (2016)

We study sequences of consumption in which the same item may be consumed multiple times. We identify two macroscopic behavior patterns of repeated consumptions. First, in a given user's lifetime, very few items live for a long time. Second, the last consumptions of an item exhibit growing inter-arrival gaps consistent with the notion of increasing boredom leading up to eventual abandonment.
Neurologic disorders in patients with small cell lung cancer (1987)

In a series of 641 patients with small cell lung cancer, 189 (29.5%) had at least one neurologic disorder either at the time of presentation or during the subsequent clinical course of the cancer. The total number of neurologic disorders was 210, which included brain metastases (75.7%), meningeal carcinomatosis (6.7%), intramedullary metastases (2.4%), epidural metastases (11.0%), hyponatremia producing CNS symptoms (3.3%), and Eaton-Lambert syndrome (1.0%). The most common signs and symptoms were motor dysfunction and confusion. The overall survival of patients with any neurologic disorder was compared to that of patients without neurologic problems. There was no difference between the survival curves for the first year and a half, but patients without neurologic complications had a greater probability of long-term survival (log-rank P = 0.03). There were no statistically significant differences when this comparison was made according to stage of disease. When a neurologic disorder related to cancer occurred, the survival time from the date of that diagnosis was usually short. The neurologic disorder was the immediate cause of death in the majority of cases. In patients who achieved a complete remission, the administration of prophylactic cranial irradiation (PCI) significantly reduced the risk of developing brain metastases as the initial site of the relapse (log-rank P = 0.0034). After adjustment for performance status and extent of disease, the survival of complete responders treated with and without PCI was not significantly different. We conclude that neurologic complications are a frequent and serious problem in patients with SCLC.
Detection of defect states in low-k dielectrics using reflection electron energy loss spectroscopy (2013)

Reflection electron energy loss spectroscopy (REELS) has been utilized to measure the band gap (Eg) and energy position of sub-gap defect states for both non-porous and porous low dielectric constant (low-k) materials. We find the surface band gap for non-porous k = 2.8–3.3 a-SiOC:H dielectrics to be ≅ 8.2 eV and consistent with that measured for a-SiO2 (Eg = 8.8 eV). Ar+ sputtering of the non-porous low-k materials was found to create sub-gap defect states at ≈ 5.0 and 7.2 eV within the band gap. Based on comparisons to observations of similar defect states in crystalline and amorphous SiO2, we attribute these sub-gap defect states to surface oxygen vacancy centers. REELS measurements on a porous low-k a-SiOC:H dielectric with k = 2.3 showed a slightly smaller band gap (Eg = 7.8 eV) and a broad distribution of defects states ranging from 2 to 6 eV. These defect states are attributed to a combination of both oxygen vacancy defects created by the UV curing process and carbon residues left in the film by incomplete removal of the sacrificial porogen. Plasma etching and ashing of the porous low-k dielectric were observed to remove the broad defect states attributed to carbon residues, but the oxygen vacancy defects remained.
Decreased miR-24-3p potentiates DNA damage responses and increases susceptibility to COPD (2020)

ABSTRACT Activation of the DNA damage response (DDR) due to chronic exposure to cigarette smoke (CS) is implicated in the pathogenesis of Chronic Obstructive Pulmonary Disease (COPD). However, not all smokers develop COPD and the pathologic consequences of CS exposure are heterogenous. Cellular mechanisms that regulate the DDR and contribute to disease progression in susceptible individuals are poorly understood. Because microRNAs are well known regulators of the DDR, we evaluated microRNA expression arrays performed on lung samples from 172 subjects with and without COPD. We identified miR-24-3p as the microRNA best correlated with radiographic emphysema (ρ=-0.353, P=1.3e-04) and validated this finding in multiple cohorts. In a CS-exposure mouse model, miR-24-3p inhibition increased emphysema severity. In human airway epithelial cells, miR-24-3p suppressed apoptosis through the BH3-only protein BIM and suppressed homology-directed DNA repair and the DNA repair protein BRCA1. Finally, we found BIM and BRCA1 were increased in COPD lung tissue and inversely correlated with miR-24-3p expression. We concluded that decreased miR-24-3p expression increases COPD susceptibility and potentiates the DDR through BIM and BRCA1.