gebhart/patrepeval huggingface dataset:
Gene modification of primary tumor cells for active immunotherapy of human breast and ovarian cancer. (1996)

We have previously shown that cationic liposomes facilitate adeno-associated virus (AAV) plasmid transfections of primary and cultured cell types. To test the clinical feasibility of using genetically modified tumor vaccines for the treatment of breast and ovarian cancers, we have constructed an expression plasmid pMP6IL2 and investigated the use of liposome-mediated gene delivery into primary, uncultured human breast and ovarian tumor cells to produce interleukin 2 (IL-2)-secreting tumor cells. We have demonstrated significant levels of IL-2 expression in tumor cell lines and primary breast and ovarian tumor cells using this AAV-based expression plasmid complexed to cationic liposomes. Transfections with the non-AAV plasmid containing the identical expression cassette as the AAV plasmid induced IL-2 expression in the tumor cell line but failed to produce IL-2 in primary tumor cells. Significant levels of IL-2 were induced with the AAV plasmid regardless of liposome compositions used for transfection. The transfected breast cell line and primary tumor cells were able to express the transgene product for up to 28 days after lethal radiation. The transfection efficiency was comparable for both the tumor cell line and primary tumor cells and ranged from 20 to 50% for both cell types as assessed by intracellular IL-2 staining. Although the primary tumor cell preparations consist of mixed population of cells, at least 40% of the tumor cells expressed the transgene as assessed by immunostaining for IL-2. The ability to efficiently express transgenes in freshly isolated, nondividing tumor cells may potentiate active immunotherapy strategies for gene-based cancer treatment.
Identification of Cryptic Products of the Gliotoxin Gene Cluster Using NMR-Based Comparative Metabolomics and a Model for Gliotoxin Biosynthesis (2011)

Gliotoxin, a major product of the gli non-ribosomal peptide synthetase gene cluster, is strongly associated with virulence of the opportunistic human pathogen Aspergillus fumigatus. Despite identification of the gli cluster, the pathway of gliotoxin biosynthesis has remained elusive, in part because few potential intermediates have been identified. In addition, previous studies suggest that knowledge of gli-dependent metabolites is incomplete. Here we use differential analysis by 2D NMR spectroscopy (DANS) of metabolite extracts derived from gli knock-out and wild-type (WT) strains to obtain a detailed inventory of gli-dependent metabolites. DANS-based comparison of the WT metabolome with that of ΔgliZ, a knock-out strain devoid of the gene encoding the transcriptional regulator of the gli cluster, revealed nine novel gliZ-dependent metabolites including unexpected structural motifs. Their identification provides insight into gliotoxin biosynthesis and may benefit studies of the role of the gli cluster in A. fumigatus virulence. Our study demonstrates the utility of DANS for correlating gene expression and metabolite biosynthesis in microorganisms.
Cosolvent-free synthesis and characterisation of poly(phenyl-co-n-alkylsilsesquioxane) and poly(phenyl-co-vinylsilsesquioxane) glasses with low melting temperatures (2020)

Thermoplastic poly(phenylsilsesquioxane) [poly(Ph-SQ)] and its copolymers with R-SQ units of linear aliphatic R groups [poly(Ph-co-R-SQ) (R = Me, Et, Pr, and Vi)] were synthesised by cosolvent-free hydrolytic polycondensation from acid-catalysed water-organotrimethoxysilane binary systems. These compounds became transparent glasses with coefficents of linear thermal expansion of ∼1 × 10-4 K-1 and Vickers hardnesses of 50-110 MPa when thermally treated at or above 100 °C. Poly(Ph-SQ) formed a fragile melt with kinetic fragility (F1/2 ⪆ 0.8) as high as that of molecular glasses. The low melting temperature and high fragility probably arise from weak attractive π interactions between phenyl groups that readily dissociate on heating, disordered Si-O frameworks, and low average molar mass (∼103 g mol-1). Proton NMR measurements confirmed other weak attractive CH/π interactions between phenyl and R groups in poly(Ph-co-R-SQ). Poly(Ph-SQ) and poly(Ph-co-R-SQ) glasses were hydrophilic and strongly bonded to a glass plate because of the presence of residual SiOH groups, while thermosetting by polycondensation was insignificant at or below 140 °C. The poly(Ph-SQ) glass was brittle, and macroscopic cracks were formed when melted on a glass plate as a result of a thermal expansion coefficient mismatch. Such crack formation was suppressed by incorporating Et-SQ, Pr-SQ, or Vi-SQ units and enhancing structural relaxation.
Cationic inorganic materials for anionic pollutant trapping and catalysis (2009)

Despite their vast industrial importance, almost all extended inorganic synthetic structures and naturally occurring minerals adopt a neutral or anionic charge. This tutorial review describes the current knowledge of inorganic materials that bear a positive charge on the host. The best-known example is the family of clays known as layered double hydroxides, and other limited examples also exist. Our research efforts have given rise to a series of new cationic inorganic materials, oxides and fluorides of heavy p-block metals. Organic-based cationic materials will also be briefly discussed. Interest in these materials is widespread, with potential applications in many areas including anionic pollutant trapping, exchange, selective catalysis, polymer hybrids and nanocomposites, and biomaterials for drug or DNA delivery.
Combined sputum hypermethylation and eNose analysis for lung cancer diagnosis (2014)

When used in concert, RASSF1A hypermethylation in sputum and exhaled breath analysis are complementary for lung cancer diagnosis, with 100% sensitivity in this series. This finding should be further validated.
In vivo metabolism of 2,6,9-trisubstituted purine-derived cyclin-dependent kinase inhibitor bohemine in mice: glucosidation as the principal metabolic route. (2001)

Synthetic cyclin-dependent kinase inhibitors have recently been referred to as effective antiproliferative agents. This study was conducted to characterize clearance of a 3H-labeled, trisubstituted purine-type inhibitor, 8-[3H]bohemine [6-benzylamino-2-(3-hydroxypropylamino)-9-isopropylpurine], in mice. Radioactivity profiles were analyzed by liquid scintillation counting and by thin layer chromatography followed by autoradiography. Metabolite structures were elucidated by mass spectrometry, NMR, and enzymatic analyses. Bohemine was rapidly and completely metabolized in vivo and disappeared from circulation during the first 60 min following intravenous administration. The metabolites were partly eliminated by the hepatobiliary tract and partly by renal excretion. The terminal hydroxyl group located at the C2 side chain of bohemine made the compound susceptible to main metabolic attacks, i.e., distinct types of conjugation reactions with glycosyl donors as well as an oxidative reaction. Other pathways were of relatively minor significance. Bohemine O-beta-D-glucoside was the most abundant metabolite to be excreted. The enzymatic mechanism responsible for bohemine glucosidation in vitro required the presence of a UDP-glucoside donor. Additional glycosidation products were observed after inclusion of UDP-glucuronide, UDP-xylose, UDP-galactose, or UDP-N-acetylglucosamine into microsomal incubates. Glycosidations occurred faster in the kidney incubates than in hepatic ones. The second principal bohemine metabolite was a carboxylic acid, 6-benzylamino-2-(2-carboxyethylamino)-9-isopropylpurine. A cytosolic, 4-methylpyrazole-sensitive alcohol dehydrogenase class I was shown to mediate oxidation of the terminal hydroxyl group of bohemine into this acid, which was the only metabolite found in the blood in significant amounts. However, it displayed only weak cyclin-dependent kinase-1-inhibitory activity (IC(50) > 100 microM) when compared with that of bohemine (IC(50) approximately 1 microM).
Direct Observation of the Relationship between Molecular Topology and Bulk Morphology for a π-Conjugated Material (2019)

High-performance organic semiconducting materials are reliant upon subtle changes in structure across different length scales. These morphological features control relevant physical properties and ultimately device performance. By combining in situ NMR spectroscopy and theoretical calculations, the conjugated small molecule TT is shown to exhibit distinct temperature-dependent local structural features that are related to macroscopic properties. Specifically, lamellar and melt states are shown to exhibit different molecular topologies associated with planar and twisted conformations of TT, respectively. This topological transformation offers a novel avenue for molecular design and control of solid-state organization.
Interactive light transport editing for flexible global illumination (2007)

The limited flexibility imposed by the physics of light transport has been a major hurdle in the use of global illumination in production rendering. It has been a common practice in computer cinematography to write custom shaders [Christensen 2003] or manually edit rendered images [Thacker 2006] to achieve lighting effects desired by directors. This process is lengthy, cumbersome and too technical for artists. To remove this hurdle, we propose a novel interface that allows users to modify light transport in a scene. Users can adjust effects of indirect lighting cast by one object onto another or paint indirect illumination on surfaces. As opposed to previous approaches such as [Schoeneman et al. 1993] our work departs from solving optimization problems in favor of more user control.
A T cell-dependent mechanism for the induction of human mucosal homing IgA-secreting plasmablasts (39.5) (2009)

Abstract Mucosal IgA secreted by local plasma cells (PCs) is a critical component of mucosal immunity. Although IgA class switching can occur at mucosal sites in a T cell-independent manner, high-affinity PCs are optimally generated in germinal centers (GCs) in a T cell-dependent fashion. However, the mechanism of how CD4+ helper T cells induce mucosal-homing IgA-PCs remains unclear. We show here that TGFb1 and IL-21, produced by follicular helper T cells (TFH), synergize to generate abundant IgA-plasmablasts (PBs) that express CCR10. In the presence of IL-21, TGFb1 promotes naive B cell proliferation and differentiation. In addition it overrides IL-21-induced IgG class switching in favor of IgA. Furthermore, in combination with IL-21, TGFb1 downregulates CXCR5, while upregulating CCR10 on PBs, enabling their exit from GCs and migration towards local mucosa. This is supported by the presence of CCR10+IgA+ cells in tonsil GCs. In conclusion, TFH, through IL-21 and TGFb1 contribute to the generation of human mucosal-homing IgA+ PCs. Thus, mucosal vaccines should aim to induce robust TFH responses.
Using Two-Photon Microscopy to Study Dynamic Kidney Processes in 4D (2005)

Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text B. A. Molitoris, "Using Two-Photon Microscopy to Study Dynamic Kidney Processes in 4D," in Frontiers in Optics, OSA Technical Digest Series (Optica Publishing Group, 2005), paper JMD1. Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
Growth of In‐doped ZnO films by metalorganic chemical vapor deposition on GaN(0001) templates (2009)

Abstract We investigated the properties of indium‐doped zinc oxide layers grown by metalorganic chemical vapor deposition on semi‐insulating GaN(0001) templates. Specular and transparent films with n‐type carrier concentrations up to 1.82 × 10 19 cm –3 , as determined by hall measurements, were grown. An undoped film grown under the same conditions had carrier concentration 5.4 × 10 17 cm –3 . An interesting trend was observed for carrier concentration dependence on In precursor flow. For low In flows, carrier concentration increased with TMI flow until a peak was reached for a TMI flow of 5 standard cubic centimetres per minute. Beyond this flow, carrier concentration decreased with increasing TMI flow rate. A sheet resistance as low as 185 ohm/sq was achieved for the indium‐doped films, which is a significant decrease from the undoped ZnO film which had a sheet resistance of 3100 ohm/sq. The results of this In doping study show that In is an effective dopant for controlling the n‐type conductivity of ZnO. (© 2009 WILEY‐VCH Verlag GmbH &amp; Co. KGaA, Weinheim)
Complexes Derived from Strong Field Ligands. XIX. Magnetic Properties of Transition Metal Derivatives of 4,4',4",4'''-Tetrasulfophthalocyanine (1965)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTComplexes Derived from Strong Field Ligands. XIX. Magnetic Properties of Transition Metal Derivatives of 4,4',4",4'''-TetrasulfophthalocyanineJames H. Weber and Daryle H. BuschCite this: Inorg. Chem. 1965, 4, 4, 469–471Publication Date (Print):April 1, 1965Publication History Published online1 May 2002Published inissue 1 April 1965https://pubs.acs.org/doi/10.1021/ic50026a007https://doi.org/10.1021/ic50026a007research-articleACS PublicationsRequest reuse permissionsArticle Views2720Altmetric-Citations470LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Evolution of Dead Lithium Growth in Lithium Metal Batteries: Experimentally Validated Model of the Apparent Capacity Loss (2019)

In this work, a physics-based model has been developed to understand how dead lithium (Li) affects the apparent capacity loss in Li metal battery cells. Specifically, the dead Li layer is modeled as a secondary layer which restricts ion transport similar to the separator. The measured dead Li thickness is used as an input to the model, and both the effective diffusion coefficient and ionic conductivity of the dead Li layer have been tuned to match the terminal voltage response of a Li-NMC 532 coin cell under galvanostatic cycling. The model captures the changes in terminal voltage and apparent cell capacity as the dead Li layer builds up. This model can be inverted to estimate the thickness and effective transport properties of the dead Li layer from the observed terminal voltage for enhanced diagnostics of the apparent capacity degradation of Li metal battery cells as well as fast-charging Li ion battery systems with graphite anodes.
Protein-protected luminescent noble metal quantum clusters: an emerging trend in atomic cluster nanoscience (2012)

Noble metal quantum clusters (NMQCs) are the missing link between isolated noble metal atoms and nanoparticles. NMQCs are sub-nanometer core sized clusters composed of a group of atoms, most often luminescent in the visible region, and possess intriguing photo-physical and chemical properties. A trend is observed in the use of ligands, ranging from phosphines to functional proteins, for the synthesis of NMQCs in the liquid phase. In this review, we briefly overview recent advancements in the synthesis of protein protected NMQCs with special emphasis on their structural and photo-physical properties. In view of the protein protection, coupled with direct synthesis and easy functionalization, this hybrid QC-protein system is expected to have numerous optical and bioimaging applications in the future, pointers in this direction are visible in the literature.
Fast resistive switching in WO 3 thin films for non-volatile memory applications (2009)

Tungsten trioxide (WO 3 ) thin films with Pt and Cu electrodes are characterized regarding the suitability for nonvolatile memory applications. Cells down to 70 × 70 nm² are fabricated by a combination of e-beam and nanoimprint lithography. The electrical measurements reveal good properties with switching currents of only 500 nA, fast SET and RESET switching down to 5 ns and retention for 104 s with a stable R OFF /R ON ratio larger than 105.
Five major nuclear ribosomal repeats represent a large and variable fraction of the genomic DNA of Picea rubens and P. mariana. (1992)

The nuclear ribosomal repeats for the 18S, 5.8S, and 26S RNAs of two closely related Picea (spruce) species were characterized by restriction mapping and Southern blot hybridization. Restriction polymorphisms were identified in the IGS and ITS sequences; however, no polymorphism was species specific. As many as five different rDNA repeat units were observed in individual genomes. The repeat size for these gymnosperms ranged from a minimum of 32 kbp to greater than 40 kbp, two- to threefold larger than the typical angiosperm rDNA unit. Slot-blot hybridizations were used to determine the nuclear rDNA copy concentration. Among P. rubens individuals threefold variation was observed in the rDNA copy concentration, and among P. mariana individuals such variation was as much as sixfold. At a size greater than 32 kbp and at a concentration averaging 1.2-1.3 x 10(4) copies/pg, the rDNA constitutes approximately 4% of the total genome. Regression analysis revealed a significant relationship between copy concentration of the rDNA repeat unit in P. rubens and geographic origins. Differences in the rDNA content in Picea could contribute to the variation, in overall genome size, that has been observed within conifer species.
Low noise characteristics of AlGaInN‐based self‐pulsating laser diodes (2008)

Abstract We have successfully realized practical self‐pulsating laser diodes (SP‐LDs) based on AlGaInN systems that exhibit low noise characteristics even with an optical feedback of 1% at a high temperature of 75 °C. Our fabricated SP‐LD employs a saturable absorber layer (SAL). We adopt new approaches to realize practical SP operation; specifically, (i) we inserted an AlGaN layer between the active layer and the SAL in order to reduce the threshold current, and (ii) we intentionally introduced reactive ion etching (RIE) damage to the SAL in order to reduce the carrier lifetime in the SAL by controlling the distance between the SAL and the dry‐etched surface. The threshold currents for the fabricated SP‐LD at 25 °C and 75 °C were 38.8 mA and 54.4 mA, respectively. We also demonstrated that it is possible to suppress the relative intensity noise (RIN &lt; –125 dB/Hz) even under the conditions of high temperature (75 °C) and an optical feedback of 1%. These results indicate that practical SP‐LDs for use in optical storage devices can be realized by inducing dry etching damage in the SAL. (© 2008 WILEY‐VCH Verlag GmbH &amp; Co. KGaA, Weinheim)
Posttrabeculectomy Anterior Subcapsular Cataract Formation Induced by Anterior Chamber Air (1993)

We retrospectively examined the effect of air, as contrasted with balanced salt solution (BSS), sodium hyaluronate, or no substance at all, used to reform the anterior chamber at the end of trabeculectomy on subsequent cataract formation in 141 consecutive patients (173 phakic eyes). The minimum follow up was 120 days. The use of air increased the incidence of anterior subcapsular cataract formation significantly. Also, the eyes that had a large air bubble on postoperative day 1 had a higher incidence of anterior subcapsular cataract than the eyes with less air on that day. We suggest that the toxicity to the lens of the oxygen in the air increased the incidence of anterior subcapsular cataract in the eyes in which air was used. We recommend that BSS or sodium hyaluronate be used instead of air to reform the anterior chamber following trabeculectomy.
218Kauth/s, 3nJ/auth SCA/ML-Resistant Privacy-Preserving Mutual Authentication Accelerator with a Crypto-Double-Coupled PUF in 4nm class CMOS (2023)

A SCA/ML-resistant secure PPMA accelerator is fabricated in 4nm class CMOS, occupying $3776 \mu \mathrm{m}^{2}$. Double-coupled crypto microarchitecture using a 128b strong PUF along with AES128 round hardware achieves CRP space of 10<sup>28</sup> with BER of 0.15%. Secure mote/server-authentication throughput of 218/435Kauth/s is demonstrated with no side-channel leakage detected after 2.5M authentications. Resistance to machine-learning attacks is demonstrated against 10M training CRPs.
The regulation of soluble receptor for AGEs contributes to carbonyl stress in schizophrenia (2016)

Our previous study showed that enhanced carbonyl stress is closely related to schizophrenia. The endogenous secretory receptor for advanced glycation end-products (esRAGE) is a splice variant of the AGER gene and is one of the soluble forms of RAGE. esRAGE is considered to be a key molecule for alleviating the burden of carbonyl stress by entrapping advanced glycation end-products (AGEs). In the current study, we conducted genetic association analyses focusing on AGER, in which we compared 212 schizophrenic patients to 214 control subjects. We also compared esRAGE levels among a subgroup of 104 patients and 89 controls and further carried out measurements of total circulating soluble RAGE (sRAGE) in 25 patients and 49 healthy subjects. Although the genetic association study yielded inconclusive results, multiple regression analysis indicated that a specific haplotype composed of rs17846798, rs2071288, and a 63 bp deletion, which were in perfect linkage disequilibrium (r<sup>2</sup> = 1), and rs2070600 (Gly82Ser) were significantly associated with a marked decrease in serum esRAGE levels. Furthermore, compared to healthy subjects, schizophrenia showed significantly lower esRAGE (p = 0.007) and sRAGE (p = 0.03) levels, respectively. This is the first study to show that serum esRAGE levels are regulated by a newly identified specific haplotype in AGER and that a subpopulation of schizophrenic patients are more vulnerable to carbonyl stress.
Achieving max-min fairness for MU-MISO with partial CSIT: A multicast assisted transmission (2015)

We address the max-min fairness design problem for a MU-MISO system with partial Channel State Information (CSI) at the Base Station (BS), consisting of an imperfect channel estimate and statistical knowledge of the estimation error, and perfect CSI at the receivers. The objective is to maximize the minimum Average Rate (AR) among users subject to a transmit power constraint. An unconventional transmission scheme is adopted where the Base Station (BS) transmits a common message in addition to the conventional private messages. In situations where the CSIT is not accurate enough to perform interference nulling, individual rates are assisted by allocating parts of the common message to different users according to their needs. The AR problem is transformed into an augmented AverageWeighted Mean Square Error (AWMSE) problem, solved using Alternating Optimization (AO). The benefits of incorporating the common message are demonstrated through simulations.
Erratum: “Proton radiation hardness of single-nanowire transistors using robust organic gate nanodielectrics” [Appl. Phys. Lett. 89, 073510 (2006)] (2006)

First Page