gebhart/patrepeval huggingface dataset:
N-Methylsulfonimidoyl-Substituted (2-Alkenyl)titanium Complexes: Application to the Synthesis of β- and δ-Sulfonimidoyl-Substituted Chiral Homoallylic Alcohols, X-ray Crystal Structure Analysis, and Fluxional Behavior (2000)

Enantiopure acyclic (E)- and (Z)-configured allylic sulfoximines have been synthesized from N,S-dimethyl-S-phenylsulfoximine and aldehydes by the addition− elimination−isomerization route through the intermediate generation of the corresponding (E)-configured vinylic sulfoximines. Isomerization of the vinylic sulfoximines with DBU preferentially afforded the corresponding (Z)-configured allylic sulfoximines, which were subsequently isomerized by DBU to preferentially yield the (E)-isomers. Titanation of lithiated (E)-configured allylic sulfoximines with ClTi(OiPr)3 furnished the corresponding bis(2-alkenyl)diisopropyloxytitanium(IV) complexes, which reacted with aldehydes in the presence of ClTi(OiPr)3 with high regio- and diastereoselectivities at the γ-position to give the corresponding (Z)-anti-configured δ-N-methylsulfonimidoyl-substituted homoallylic alcohols in good yields. In the absence of ClTi(OiPr)3 at low temperatures, only one allylic moiety of the bis(alkenyl)diisopropyloxytitanium complex is transferred to the aldehyde. In this way, a cyclic lithiated allylic sulfoximine has been converted with high regio- and diastereoselectivity to the corresponding homoallylic alcohols bearing a vinylic sulfonimidoyl group. Titanation of lithiated (E)- and (Z)-configured allylic sulfoximines with ClTi(NEt2)3 afforded the corresponding mono(2-alkenyl)tris(diethylamino)titanium(IV) complexes, which reacted with aldehydes with moderate to high regioselectivities and high diastereoselectivities preferentially at the α-position to give the corresponding syn-configured β-N-methylsulfonimidoyl-substituted homoallylic alcohols along with the (Z)-anti-configured δ-N-methylsulfonimidoyl-substituted homoallylic alcohols in good yields. In this way, the cyclic lithiated allylic sulfoximine was converted with high regio- and diastereoselectivity to the corresponding isomeric homoallylic alcohols bearing an allylic sulfonimidoyl group. In the case of mono(alkenyl)tris(diethylamino)titanium(IV) complexes, the regioselectivity of their reactions with aldehydes has been found to depend on the size of the substituent at the CC double bond and the aldehyde, as well as on the configuration of the double bond. Reaction of racemic lithiated N-methyl-S-(3,3-diphenyl-2-propenyl)-S-phenylsulfoximine with ClTi(OiPr)3 afforded the corresponding bis(alkenyl)diisopropyloxytitanium(IV) complex. X-ray structure analysis revealed a distorted octahedral cis,cis,cis-configured bis(2-alkenyl)diisopropyloxytitanium(IV) complex, in which the allylic moieties are coordinated in a bidentate fashion through C-α and the N atom to the Ti atom, both having the relative configuration RSSC. In solution, the titanium complex shows fluxional behavior, which is characterized by topomerization of the isopropyloxy groups and allylic moieties. The exchange of the latter occurs with retention of the configuration at C-α.
Effective time‐domain approach for the assessment of the stability characteristics and other non‐linear effects of RF and microwave circuits (2019)

This study describes a systematic approach for the stability analysis of RF and microwave non‐linear circuits in the time‐domain and that can be useful also for the verification of other non‐linearities, like intermodulation. The time‐domain analysis is the most reliable approach for the evaluation of complex non‐linear phenomena but, in general, the transient behaviour of non‐linear circuits is difficult to verify at high frequencies, where distributed elements are common. The solution here addressed overcomes this limitation and it may be applied, without restrictions, also to monolithic microwave integrated circuits and EM‐based designs. Examples of application to hybrid prototypes are provided, and the comparison between simulations and measurements illustrates the accuracy and reliability of the proposed approach.
Human POT1 Prevents Severe Telomere Instability Induced by Homology Directed DNA Repair (2020)

SUMMARY The evolutionarily conserved POT1 protein binds single stranded G-rich telomeric DNA and has been implicated in contributing to telomeric DNA maintenance and the suppression of DNA damage checkpoint signaling. Here, we explore human POT1 function through genetics and proteomics discovering that the complete absence of POT1 leads to severe telomere maintenance defects that had not been anticipated from previous depletion studies. Conditional deletion of POT1 in HEK293E cells gives rise to rapid telomere elongation and length heterogeneity, branched telomeric DNA structures, telomeric R-loops and telomere fragility. We determine the telomeric proteome upon POT1-loss implementing an improved telomeric chromatin isolation protocol. We identify a large set of proteins involved in nucleic acid metabolism that engage with telomeres upon POT1-loss. Inactivation of the homology directed repair machinery suppresses POT1-loss mediated telomeric DNA defects. Our results unravel as major function of human POT1 the suppression of telomere instability induced by homology directed repair.
ChemInform Abstract: Total Synthesis of (‐)‐Hikizimycin Employing the Strategy of Two‐Directional Chain Synthesis. (1992)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
3D Nanoprinted Liquid-Core-Shell Microparticles (2020)

The ability to fabricate core-shell microparticles with high control over the three-dimensional (3D) microarchitecture of each particle offers unique potential to advance applications such as cell/tissue engineering, diagnostics, and drug delivery. Despite significant progress, current barriers include undesired polydispersity (e.g., for particle size and shape) as well as limited design customization with respect to the 3D geometric complexity of individual microparticles. To address such challenges, here we introduce a novel strategy that leverages the submicron-scale additive manufacturing (or “3D printing”) technology, “direct laser writing (DLW)”, to fabricate core-shell microparticles with liquid-phase cores and individually tunable shell architectures. This approach consists of three fundamental steps: (i) DLW-based printing of a photomaterial shell with a top orifice, (ii) vacuum-loading of a liquid-phase core, and then (iii) DLW-based printing of a “cap” atop the orifice to complete the shell (while sealing the core). In this work, we investigated the relationships between the initial shell orifice size and microfluidic core encapsulation efficacy. Fabrication and experimental results for microfluidic vacuum-loading of cores comprising a methylene blue-dyed aqueous solution revealed that shell orifice diameters (D) of 1.71±0.14 μm exhibited poor core-loading performance, whereas D ≥ 3.61±0.13 μm all led to effective core-loading. Results for fluorescence quantification after the final encapsulation step, however, revealed that each increase of approximately 2 μm in D from 3.61±0.13 μm up to 11.69±0.16 μm corresponded to a significant reduction in retention performance of the microfluidic core. In combination, the presented methodology opens new avenues for core-shell microparticle design and manufacturing, and in turn, emerging applications enabled by such capabilities.
1P1-C11 Automated Cell Stiffness Measurement by using Two-Fingered Microhand with Micro Force Sensors(Bio Assembler for 3D Cellular System Innovation) (2013)

In the field of life science, many experiments and analyses of cell is performed. One of the cell analysis focuses on cell stiffness. Cell stiffness depends on cell's status, thus it is important approach for cell analysis to measure cell tiffness. However, to measure the cell stiffness, it is necessary to manipulate microscopic cell and to measure nano or pico newton force. To solve these problems, cell stiffness measurement method by using two-fingered microhand has been researched. In this paper, automated cell stiffness measurement by using two-fingered microhand with micro force sensor is described. By measuring cell stiffness automatically, stable and reproducible measurement is realized.
Templating silver nanoparticle growth using phosphonated calixarenes (2009)

Very small ( approximately 2 nm) silver nanoparticles can be readily prepared using p-phosphonated calixarenes as templating agents and surfactants, at the same time allowing the use of hydrogen gas as an environmentally benign reductant.
Efficient Spectrum Sensing With Dyadic Tree Partitioning (2010)

A low-complexity spectrum-sensing method that is based on dyadic (binary) tree partitioning is proposed. For this purpose, first, a representation of the spectrum occupation with a dyadic tree is developed. Then, the accuracy of this representation is evaluated through a rate-distortion criterion. Using this criterion, a pruning algorithm for the dyadic tree that minimizes the number of sensing operations for a predetermined value of sensing accuracy is conceived. After tailoring the pruning algorithm to the missed-detection and collision requirements of the opportunistic access, an adaptive version that performs efficient sensing in slowly varying environments is proposed. The proposed algorithm is tested on real spectrum measurements taken in the 400-910-MHz range. The sensitivity of the proposed algorithm to sensing imperfections is also evaluated. The obtained results demonstrate that the proposed algorithm provides a mean distortion of 11.4% and a mean collision probability of 5.6% for sensing only 30% of the total spectrum. A comparison with conventional energy-based detection highlights a gain of 50% in terms of the number of required measurements per channel in the low-signal-to-noise-ratio (SNR) region, where conventional energy detection is known to have poor detection performance.
Fundamental studies of microscopic wetting on organic surfaces. 2. Interaction of secondary adsorbates with chemically textured organic monolayers (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFundamental studies of microscopic wetting on organic surfaces. 2. Interaction of secondary adsorbates with chemically textured organic monolayersLawrence H. Dubois, Bernard R. Zegarski, and Ralph G. NuzzoCite this: J. Am. Chem. Soc. 1990, 112, 2, 570–579Publication Date (Print):January 1, 1990Publication History Published online1 May 2002Published inissue 1 January 1990https://pubs.acs.org/doi/10.1021/ja00158a013https://doi.org/10.1021/ja00158a013research-articleACS PublicationsRequest reuse permissionsArticle Views872Altmetric-Citations232LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A Comparative Study of the Main Pulmonary Artery and Ascending Aorta Biomechanical Behavior (2011)

During the prenatal period a state of physiologic pulmonary hypertension exists due to the equalization of pressures by the patent ductus arteriosus, resulting in similar wall thickness of the ascending aorta (AA) and main pulmonary artery (MPA). After birth, as the ductus arteriosus closes and pulmonary arterial pressure decreases, attenuation of medial smooth muscle occurs such that the ratio of medial thickness to external diameter decreases from about 25% in fetuses to less than 10% in infants 3 to 6 months of age. After the first year of life, thickness of the MPA is normally less than half that of the adjacent ascending aorta, although the diameters of the two great arteries remain the same relative to one another [1]. During homeostatic conditions, the total pulmonary and systemic blood flows are essentially identical. In spite of their comparable blood flow rate and common embryologic origin, the anatomic characteristics of these two segments of the cardiovascular system differ substantially [2]. Futhremore, both these arteries are affected by many congenital abnormalities and also are subject to hypertension. Knowledge of the normal biomechanical properties of these great arteries is important for surgical treamtment, angioplasty, and tissue engineering. It can also provide insight into the disease processes and is a prerequisite to the study of mechanical behavior during disease conditions. In this study we characterized the biaxial mechanical behavior of both arteries as a function of location, which has not been previously performed in the pulmonary trunk.
Attenuation‐emission alignment in cardiac PET/CT based on consistency conditions (2010)

The proposed alignment procedure offers an automatic method to reduce attenuation correction artifacts in cardiac PET/CT and provides a viable supplement to subjective manual realignment tools.
Supplementary Video S2 from Unexpected Activities in Regulating Ciliation Contribute to Off-target Effects of Targeted Drugs (2023)

&lt;p&gt;Supplementary Video S2. Time-lapse images of hTERT-RPE1-Arl13b-GFP cells starved in serum-free Opti-MEM for 48 hours, then incubated for 3 hours in 20% FBS.&lt;/p&gt;
Phosphonamidates are the first phosphorus-based zinc binding motif to show inhibition of β-class carbonic anhydrases from bacteria, fungi, and protozoa (2019)

A primary strategy to combat antimicrobial resistance is the identification of novel therapeutic targets and anti-infectives with alternative mechanisms of action. The inhibition of the metalloenzymes carbonic anhydrases (CAs, EC 4.2.1.1) from pathogens (bacteria, fungi, and protozoa) was shown to produce an impairment of the microorganism growth and virulence. As phosphonamidates have been recently validated as human α-CA inhibitors (CAIs) and no phosphorus-based zinc-binding group have been assessed to date against β-class CAs, herein we report an inhibition study with this class of compounds against β-CAs from pathogenic bacteria, fungi, and protozoa. Our data suggest that phosphonamidates are among the CAIs with the best selectivity for β-class over human isozymes, making them interesting leads for the development of new anti-infectives.
Feasibility Study of an Optical Caustic Plasmonic Light Scattering Sensor for Human Serum Anti-Dengue Protein E Antibody Detection (2017)

Antibody detection and accurate diagnosis of tropical diseases is essential to help prevent the spread of disease. However, most detection methods lack cost-effectiveness and field portability, which are essential features for achieving diagnosis in a timely manner. To address this, 3D-printed oblate spheroid sample chambers were fabricated to measure green light scattering of gold nanoparticles using an optical caustic focus to detect antibodies. Scattering signals of 20-200 nm gold nanoparticles using a green laser were compared to green light emitting diode (LED) light source signals and to Mie theory. The change in signal from 60 to 120 nm decreased in the order of Mie Theory > optical caustic scattering > 90° scattering. These results suggested that conjugating 60 nm gold nanoparticles and using an optical caustic system to detect plasmonic light scattering, would result in a sensitive test for detecting human antibodies in serum. Therefore, we studied the light scattering response of conjugated gold nanoparticles exposed to different concentrations of anti-protein E antibody, and a feasibility study of 10 human serum samples using dot blot and a handheld optical caustic-based sensor device. The overall agreement between detection methods suggests that the new sensor concept shows promise to detect gold nanoparticle aggregation in a homogeneous assay. Further testing and protocol optimization is needed to draw conclusions on the positive and negative predictive values for this new testing system.
Regiospecific Oxidative Nitration of 3,4-Dihydro-6,7-disubstituted Quinoxaline-2(1H)-ones Gives 1,4-Dihydro-5-nitro-6,7-disubstituted Quinoxaline-2,3-diones, Potent Antagonists at the NMDA/Glycine Site (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRegiospecific Oxidative Nitration of 3,4-Dihydro-6,7-disubstituted Quinoxaline-2(1H)-ones Gives 1,4-Dihydro-5-nitro-6,7-disubstituted Quinoxaline-2,3-diones, Potent Antagonists at the NMDA/Glycine SiteSunil M. Kher, Sui Xiong Cai, Eckard Weber, and John F. W. KeanaCite this: J. Org. Chem. 1995, 60, 18, 5838–5842Publication Date (Print):September 1, 1995Publication History Published online1 May 2002Published inissue 1 September 1995https://pubs.acs.org/doi/10.1021/jo00123a020https://doi.org/10.1021/jo00123a020research-articleACS PublicationsRequest reuse permissionsArticle Views415Altmetric-Citations14LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
Optical Characterization of Planar Photonic Crystal Structures with Elliptically-Elongated Veins: A Generalized Fourier-Mathieu Multipole Expansion Technique (2006)

An efficient analytical approach based on an extension of the scattering-matrix technique is presented for characterizing planar photonic crystal resonant-cavities composed of elliptical veins. Extensive numerical results for various lattices are presented.
Solid-Phase Synthesis of 5-Biphenyl-2-yl-1H-tetrazoles (2004)

The combinatorial synthesis of novel biphenyl tetrazoles is described. Key steps include the simultaneous biphenyl formation and phenol deallylation under Suzuki cross-coupling conditions as well as the tetrazole ring formation on solid support. A representative library of 20 biphenyl tetrazoles was synthesized.
A stochastic deconvolution method to reconstruct insulin secretion rate after a glucose stimulus (1996)

Insulin secretion rate (ISR) is not directly measurable in man but it can be reconstructed from C-peptide (CP) concentration measurements by solving an input estimation problem by deconvolution. The major difficulties posed by the estimation of ISR after a glucose stimulus, e.g., during an intravenous glucose tolerance test (IVGTT), are the ill-conditioning of the problem, the nonstationary pattern of the secretion rate, and the nonuniform/infrequent sampling schedule. In this work, a nonparametric method based on the classic Phillips-Tikhonov regularization approach is presented. The problem of nonuniform/infrequent sampling is addressed by a novel formulation of the regularization method which allows the estimation of quasi time-continuous input profiles. The input estimation problem is stated into a Bayesian context, where the a priori known nonstationary characteristics of ISR after the glucose stimulus are described by a stochastic model. Deconvolution is tackled by linear minimum variance estimation, thus allowing the derivation of new statistically based regularization criteria. Finally, a Monte-Carlo strategy is implemented to assess the uncertainty of the estimated ISR arising from CP measurement error and impulse response parameters uncertainty.
Continuous cytometric bead processing within a microfluidic device for bead based sensing platforms (2007)

A microfluidic device for continuous biosensing based on analyte binding with cytometric beads is introduced. The operating principle of the continuous biosensing is based on a novel concept named the "particle cross over" mechanism in microfluidic channels. By carefully designing the microfluidic network the beads are able to "cross-over" from a carrier fluid stream into a recipient fluid stream without mixing of the two streams and analyte dilution. After crossing over into the recipient stream, bead processing such as analyte-bead binding may occur. The microfluidic device is composed of a bead solution inlet, an analyte solution inlet, two washing solution inlets, and a fluorescence detection window. To achieve continuous particle cross over in microfluidic channels, each microfluidic channel is precisely designed to allow the particle cross over to occur by conducting a series of studies including an analogous electrical circuit study to find optimal fluidic resistances, an analytical determination of device dimensions, and a numerical simulation to verify microflow structures within the microfluidic channels. The functionality of the device was experimentally demonstrated using a commercially available fluorescent biotinylated fluorescein isothiocyanate (FITC) dye and streptavidin coated 8 microm-diameter beads. After, demonstrating particle cross over and biotin-streptavidin binding, the fluorescence intensity of the 8 microm-diameter beads was measured at the detection window and linearly depends on the concentration of the analyte (biotinylated FITC) at the inlet. The detection limit of the device was a concentration of 50 ng ml(-1) of biotinylated FITC.
More Sensitive Methods for Detection of Antibody to Mycoplasma pneumoniae (1973)

Journal Article More Sensitive Methods for Detection of Antibody to Mycoplasma pneumoniae Get access Helmut Brunner, Helmut Brunner Search for other works by this author on: Oxford Academic PubMed Google Scholar Robert L. Horswood, Robert L. Horswood Search for other works by this author on: Oxford Academic PubMed Google Scholar Robert M. Chanock Robert M. Chanock Search for other works by this author on: Oxford Academic PubMed Google Scholar The Journal of Infectious Diseases, Volume 127, Issue Supplement_1, March 1973, Pages S52–S55, https://doi.org/10.1093/infdis/127.Supplement_1.S52 Published: 01 March 1973
Resonant cavity enhanced AlGaAs/GaAs heterojunction phototransistors with an intermediate InGaAs layer in the collector (1990)

Gain and spectral response of heterojunction phototransistors (HPTs) having a thin (0.1 μm) InGaAs strained absorbing layer in the collector has been investigated. Low dark current ∼ 5 pA (1×10−8 A/cm2) and large optical gain as high as 500 were observed. A resonant cavity composed of an AlAs/GaAs buried mirror structure (reflectivity R=0.9) and the epilayer surface (R=0.3) was used to enhance the otherwise small quantum efficiency η (at InGaAs absorption wavelength). For a 1000 Å absorbing layer an improvement of η from 6.7 to 43% (6.4-fold) was demonstrated, in agreement with calculations, through the spectral analysis of the HPTs with and without resonant cavities.