gebhart/patrepeval huggingface dataset:
TNF-alpha and IFN-gamma induce expression of nitric oxide synthase in cultured rat medullary interstitial cells (1995)

Cytokines increase the expression of the inducible (type II) nitric oxide synthase (NOS) in macrophages, liver, and renal epithelial cells. Previously, we found that cultured rat medullary interstitial cells (RMIC) contain high levels of soluble guanylyl cyclase. To determine whether these cells can also produce NO, we studied the effects of tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) on NO production, NOS II mRNA, and NOS II protein expression. Both TNF-alpha and IFN-gamma, in the presence of a low concentration of the other cytokine, caused dose-dependent increases in NO production. Exposure to TNF-alpha and IFN-gamma stimulated the production of NOS II mRNA, as determined by Northern blotting. Restriction mapping of reverse transcription-polymerase chain reaction products indicated that normal cells contained macrophage NOS II, whereas cytokine-stimulated cells contained primarily vascular smooth muscle NOS II and some macrophage NOS II. The appearance of NOS II protein was demonstrated by Western blotting. RMIC cell guanosine 3',5'-cyclic monophosphate accumulation increased 129-fold in response to the cytokines. NOS inhibitors decreased nitrite production. We conclude that 1) TNF-alpha and IFN-gamma induce the expression of vascular smooth muscle NOS II and production of NO in RMIC, and 2) NO acts as an autocrine activator of the soluble guanylyl cyclase in RMIC.
C++ Math Class Library: Permutations, Partitions, Calculators, and Gaming (1994)

Getting Started Conventions and Notations FOUNDATION MODULES Binomials Compare Utility Classes GAMES Regions Trapezoidal Regions HexGrid CALCULATION MODULES Calculator Functions Applications of Function Permutations Permutation Applications Partitions Partition Applications Bibliography Index.
Changes classification in M2 models. (2007)

Abstract: As Model Driven Software Development gains attention, its utilization be-come more feasible in large scale software projects. The metamodels (M2 models) are important artifacts of the MDD process. These models capture the modeler’s un-derstanding of the problem domain. However, the process of problem domain un-derstanding is not necessarily completed, as the M2 models is being delivered to its consumers. Therefore, the changes of M2 models are inherent to the modeling process. Therefore, the M1 models can erode, when not backwards-compatible changes are be-ing introduced to M2 models. The problem of M1 model erosion in face of M2 model changes becomes a definitive one, when MDD is applied in large scale development projects. We therefore propose a structured M1 model migration, driven by M2 model changes. In this paper we present the classification of M2 model changes, in regard to their impact on corresponding M1 models. To illustrate our considerations, we use the example of super type associations in context of the Eclipse Modeling Framework. 1
Crystallinity-based product design: Utilizing the polymorphism of isotactic PP homo- and copolymers (2015)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation Markus Gahleitner, Daniela Mileva, René Androsch, Dietrich Gloger, Davide Tranchida, Martina Sandholzer, Petar Doshev; Crystallinity-based product design: Utilizing the polymorphism of isotactic PP homo- and copolymers. AIP Conf. Proc. 17 December 2015; 1695 (1): 020003. https://doi.org/10.1063/1.4937281 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAIP Publishing PortfolioAIP Conference Proceedings Search Advanced Search |Citation Search
SERVA COLLAGENASE NB1: COMPARISON WITH LIBERASE HI AND INTERLOT VARIABILITY (2010)

Misawa, R.; Miki, A.; Barker, S.; Mita, A.; Ricordi, C.; Kahn, A.; Pileggi, A.; Molano, R. D.; Miyagawa, S.; Inverardi, L.; Alejandro, R.; Ichii, H. Author Information
Untangling purinergic regulation through the stellate ganglion (2023)

Sympathetic nerve overactivity is a key contributor to cardiovascular diseases, and related morbidity and mortality. Denervation of the stellate ganglion has been explored as a therapeutic strategy for heart failure, but there is a need for targeted, non-invasive approaches. Upregulation of P2X3 purinergic receptors in stellate ganglia of Spontaneously Hypertensive (SHR) compared to Wistar rats (16-week-old), revealed by RNASeq transcriptional profiling, leads us to hypothesize that purinergic signalling may play a role in the excessive sympathetic drive to the heart in cardiovascular disease.Cardiac effects of stellate ganglion purinergic stimulation were investigated in the working heart-brainstem preparation of Wistar rats (4-5 week old). Microinjection of ATP (100ng) directly into the right stellate ganglion increased heart rate (Wistar 13±6 Δbpm, n=6; SHR 25±8 Δbpm, n=2). This tachycardia was attenuated by either systemic P2X3 inhibition with AF-353 (Wistar 2±2 Δbpm, n=4), or abolished by denervation of the cardiac sympathetic nerve emanating from the stellate ganglion. However, bradycardic responses were also observed (Wistar -24 ± 12 bpm, n=5), likely indicating contrasting signalling by purinergic components such as adenosine.These findings were echoed in isolated post-ganglionic sympathetic neurones from the stellate ganglia of Wistar rats (4-6 weeks), wherein ATP evoked a significant increase (Median; 0.21) in [Ca 2+ ] i as measured by Fura-2AM imaging (n=15, Wilcoxon matched-pairs test; p&lt;0.0001) with a high degree of variability suggestive of responding and non-responding cells. This ATP-induced calcium transient was inhibited by specific P2X3 receptor antagonism with either NF-10 or AF-130.Further, immunofluorescence analysis shows clustering of neurones expressing P2X3 within the stellate ganglion.Stellate ganglion P2X3 purinergic receptors contribute to cardiac chronotropic regulation, with apparent heterogeneity in neuronal expression and differential purinergic responses. P2X3 receptor overexpression is likely to contribute to cardiac sympathetic overactivity and hypertension, making them a promising novel therapeutic target. Funding: Support provided by Health Research Council of New Zealand. This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.
Integration of UWB and Wireless Pressure Mapping in Surgical Navigation (2009)

Wireless technologies are becoming more prevalent in hospital environments. An ultra-wideband (UWB) indoor tracking system is outlined, which has dynamic 3-D real-time root-mean-square error in the range of 5.24-6.37 mm using line-of-sight signals in different experiments. This high 3-D accuracy opens up many new applications to UWB indoor wireless positioning, which includes its use for tracking smart surgical tools and its ability to register relevant objects in the scene such as a spacer block for real-time pressure mapping of the femoral condyles. Experimental results quantifying the operating room (OR) environment for UWB transmission fit to the IEEE 802.15.4a channel model are included. A simulation of our UWB positioning system with interference from an IEEE 802.11a source shows the need for a front-end bandpass filter at our UWB receiver. Both microcantilever and microelectromechanical systems-based wireless pressure sensors are presented including quantitative performance metrics (e.g., hysteresis, sensitivity). The data for these sensors is transmitted over the 315- and 433.92-MHz telemetry bands. These bands are examined for their performance in the OR.
Recursive Approach in Sparse Matrix LU Factorization (2001)

This paper describes a recursive method for the LU factorization of sparse matrices. The recursive formulation of common linear algebra codes has been proven very successful in dense matrix computations. An extension of the recursive technique for sparse matrices is presented. Performance results given here show that the recursive approach may perform comparable to leading software packages for sparse matrix factorization in terms of execution time, memory usage, and error estimates of the solution.
Design and Analysis of Dual Band Tapered-Fed Monopole Antenna for 5G and Satellite Applications (2021)

A tapered-fed monopole antenna with loading of solid circle and parallel open-ended stubs (POES) for 5G and satellite application is proposed. The loading of solid circle at the centre and four POES symmetrically on left and right side of tapered feed line induces a lower band (FL) of (3.28 - 3.54 GHz) and upper band (FU) of (8.00-10.85 GHz) for operating in 5G and satellite applications simultaneously. The structure has a compact designed footprint of 40 × 40 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> and developed using FR-4 substrate having a thickness of 0.8mm. Furthermore, the antenna structure offers a gain above 2.5 dBi and efficiency above 80% in both the operating bands to meet the application requirements of 5G and satellite frequency bands.
An Isomünchnone-Based Method for the Synthesis of Highly Substituted 2(1H)-Pyridones (1999)

1-(Benzenesulfonyl-diazoacetyl)-pyrrolidin-2-one was prepared by a diazo transfer of 1-(benzenesulfonylacetyl)-pyrrolidin-2-one with p-acetamidobenzenesulfonyl azide and triethylamine. Treatment of the diazoimide with a catalytic quantity of rhodium(II) acetate resulted in the formation of an isomünchnone dipole, which underwent bimolecular trapping with various dipolarophiles in high yield. The initially formed cycloadducts were not isolable or observed, as they all readily underwent ring opening to give the 3-hydroxy-2(1H)-pyridone ring system. The 3-hydroxy-2(1H)-pyridones were readily converted to the corresponding triflates, which function as suitable substrates in various types of palladium-catalyzed cross-coupling reactions. Commercial tetrakis(triphenylphoshine)palladium was found to be a particularly effective catalyst for the cross-coupling with aryl, vinyl, and acetylenic partners. An application of the method to the synthesis of the indolizidine alkaloid (±)-ipalbidine was carried out in eight steps in 17% overall yield. The angiotensin-converting enzyme inhibitor (−)-A58365A was also synthesized by a process based on the [3 + 2]-cycloaddition reaction of a phenylsulfonyl substituted isomünchnone intermediate. The starting material for this process was prepared from l-pyroglutamic acid and involved using a diazo phenylsulfonyl substituted pyrrolidine imide. Treatment of the diazoimide with Rh2(OAc)4 in the presence of methyl vinyl ketone afforded a 3-hydroxy-2-pyridone derivative, which was subsequently converted to the ACE inhibitor in six additional steps.
Comparison of Responses to Adrenomedullin and Calcitonin Gene‐Related Peptide in the Feline Erection Model (1997)

The purpose of the present study was to investigate the effects of intracavernosal injections of adrenomedullin (ADM) and calcitonin gene-related peptide (CGRP), two structurally similar peptides, on penile erection in the anesthetized cat. Erectile responses to ADM and CGRP were compared with responses to a standard drug combination (1.65 mg papaverine, 25 microg phentolamine, and 0.5 microg prostaglandin E1 [PGE1]). Intracavernosal injections of ADM (0.1-3 nmol) and CGRP (0.01-0.3 nmol) induced erection in a dose-dependent manner. The maximal increase in intracavernosal pressure in response to ADM was a 75% increase, while the maximal response to CGRP was comparable to that induced by the reference combination, and the maximal increase in penile length was comparable with ADM, CGRP, and the standard drug combination. The duration of the maximal pressure increase and the total duration of the response to ADM and CGRP were more abbreviated than with the control combination, and systemic blood pressure was reduced significantly after administration of CGRP, the control combination, and the higher doses of ADM. The nitric oxide synthase inhibitor, L-NAME, and the K+(ATP)-channel antagonist, glybenclamide, had no effect on the erectile response to CGRP or ADM. The CGRP receptor antagonist CGRP(8-37) attenuated the erectile response to CGRP but not to ADM. These data suggest that the erectile responses to ADM and CGRP are not mediated by nitric oxide release or the opening of K+(ATP) channels, two mechanisms reported to be involved in penile erection, and that CGRP and ADM induce penile erection by activating different receptors.
Chemical and toxicological characterization of residential oil burner emissions: I. Yields and chemical characterization of extractables from combustion of No. 2 fuel oil at different Bacharach Smoke Numbers and firing cycles. (1987)

Particulates and complex organic mixtures were sampled from the exhaust of a flame retention head residential oil burner combusting No. 2 fuel oil at three firing conditions: continuous at Bacharach Smoke No. 1, and cyclic (5 min on, 10 min off) at Smoke Nos. 1 and 5. The complex mixtures were recovered by successive Soxhlet extraction of filtered particulates and XAD-2 sorbent resin with methylene chloride (DCM) and then methanol (MeOH). Bacterial mutagenicity [see Paper II (8)] was found in the DCM extractables. Samples of DCM extracts from the two cyclic firing conditions and of the raw fuel were separated by gravity column chromatography on alumina. The resulting fractions were further characterized by a range of instrumental methods. Average yields of both unextracted particulates and of DCM extractables, normalized to a basis of per unit weight of fuel fired, were lower for continuous firing than for cyclic firing. For cyclic firing, decreasing the smoke number lowered the particulates emissions but only slightly reduced the average yield of DCM extractables. These and similar observations, here reported for two other oil burners, show that adjusting the burner to a lower smoke number has little effect on, or may actually increase, emissions of organic extractables of potential public health interest. Modifications of the burner firing cycle aimed at approaching continuous operation offer promise for reducing the amount of complex organic emissions. Unburned fuel accounted for roughly half of the DCM extractables from cyclic firing of the flame retention head burner at high and low smoke number. Large (i.e., greater than 3 ring) polycyclic aromatic hydrocarbons (PAH) were not observed in the DCM extractables from cyclic firing. However, nitroaromatics, typified by alkylated nitronaphthalenes, alkyl-nitrobiphenyls, and alkyl-nitrophenanthrenes were found in a minor subfraction containing a significant portion of the total mutagenic activity of the cyclic low smoke samples (8). Oxygen-containing PAH, typified by phenalene-1-one and its alkyl derivatives, are important mutagens from cyclic firing at high smoke conditions. Thus, oil burner effluents differ markedly from those of several other combustors, including the automotive diesel engine, where multiring PAH, typified by fluoranthene and alkylated phenanthrenes, account for a significant portion of the effluent mutagenicity. Implications for combustion and emissions source identification are discussed.
Effects of Phospholipid Composition on Adjuvant Efficiency of Liposomes (1995)

Phospholipids with various transition temperatures, including egg phosphatidyl lecithine (EPL), hydrogenated egg phosphatidyl lecithine (HEPL), dimyristoyl phosphatidylcholine (DMPC) and dipalmitoyl phosphatidylcholine (DPPC), and cholesterol (C), stearylamine (SA) and dicetylphosphate (DCP) were used in various molar proportions for the preparation of multilamellar liposomes by the dehydration-rehydration method. BALB/c mice were then immunized and reimmunized with BSA entrapped in the liposomes. No significant effects of transition temperatures of the phospholipids or of surface charge on the intensity of antibody responses were demonstrable. The strongest antibody responses were found in mice immunized with multilamellar liposomes containing in their structures EPL, HEPL or DMPC combined with C, DCP and/or SA, which also showed a high entrapment stability when incubated in vitro in blood serum.
Effects of Recombinant Human Interleukin 7 on T-Cell Recovery and Thymic Output in HIV-Infected Patients Receiving Antiretroviral Therapy: Results of a Phase I/IIa Randomized, Placebo-Controlled, Multicenter Study (2012)

NCT0047732.
Allele-specific gene expression patterns in primary leukemic cells reveal regulation of gene expression by CpG site methylation (2008)

To identify genes that are regulated by cis-acting functional elements in acute lymphoblastic leukemia (ALL) we determined the allele-specific expression (ASE) levels of 2, 529 genes by genotyping a genome-wide panel of single nucleotide polymorphisms in RNA and DNA from bone marrow and blood samples of 197 children with ALL. Using a reproducible, quantitative genotyping method and stringent criteria for scoring ASE, we found that 16% of the analyzed genes display ASE in multiple ALL cell samples. For most of the genes, the level of ASE varied largely between the samples, from 1.4-fold overexpression of one allele to apparent monoallelic expression. For genes exhibiting ASE, 55% displayed bidirectional ASE in which overexpression of either of the two SNP alleles occurred. For bidirectional ASE we also observed overall higher levels of ASE and correlation with the methylation level of these sites. Our results demonstrate that CpG site methylation is one of the factors that regulates gene expression in ALL cells.
Laparoscopic right hepatectomy for living donor (2019)

Purpose of review Many institutions have started or are planning to start a purely laparoscopic right hepatectomy (PLRH) for adult living donor recipients but the experience is relatively very limited. The present review will look at the current status of PLRH and go over some of the technical details important for a safe operation. Necessary elements and different strategies to start a safe and reproducible PLRH program will be discussed. Recent findings Several publications with a relatively large number of cases have been published in the last few years. The initial results of PLRH seem to be comparable to open donor hepatectomy when performed by surgeons with sufficient expertise laparoscopic and donor hepatectomy. Summary With the ongoing accumulation of experience in laparoscopic liver surgery and living donor liver transplantation, it is most likely that PLRH will be performed more widely than the present time. Institutions should implement a step-by-step approach with proctorship, standardization of surgical procedures and a balanced selection criterion for donors for a safe transition from open method to a PLRH program.
L’hepcidine :un nouveau regard sur le métabolisme du fer (2005)

Comme le demontre bien cette citation de Jacques Prevert, si le fer porte la notion de force, de resistance, le fait qu’il rouille nous previent du danger : le fer est bon pour la sante mais en quantite limitee! Un organisme « rouille », surcharge en fer, c’est l’hemochromatose. Dans cette maladie genetique tres frequente, le fer en trop grande quantite et surtout le fer sous la forme soluble (Fe 2+) entraine la formation de radicaux libres par la reaction de Fenton (Fe 2+ + H 2O 2 -> Fe 3+ + .OH + OH -). L’accumulation du fer s’avere donc nefaste a la fonction de l’organe dans lequel il s’accumule entrainant, cirrhose, insuffisance cardiaque, diabete, arthrite, perturbations hormonales... C’est pourquoi en situation physiologique, le fer libre Fe 2+ est rare car il est le plus souvent oxyde et lie a des proteines : la transferrine circulante et la ferritine cellulaire. En l’absence de reelle voie d’excretion du fer (les pertes se font par la desquamation et les saignements), l’organisme doit reguler finement son entree de fer afin de compenser juste la perte de fer quotidienne, soit 2 mg sur les 20 mg disponibles dans une alimentation normale. Pendant 40 ans, ce mecanisme de regulation du metabolisme du fer est reste inconnu. De meme, les proteines permettant l’entree du fer au niveau du duodenum ne sont connues que depuis peu.
Connecting the clinical IT infrastructure to a service-oriented architecture of medical devices (2017)

The new medical device communication protocol known as IEEE 11073 SDC is well-suited for the integration of (surgical) point-of-care devices, so are the established Health Level Seven (HL7) V2 and Digital Imaging and Communications in Medicine (DICOM) standards for the communication of systems in the clinical IT infrastructure (CITI). An integrated operating room (OR) and other integrated clinical environments, however, need interoperability between both domains to fully unfold their potential for improving the quality of care as well as clinical workflows. This work thus presents concepts for the propagation of clinical and administrative data to medical devices, physiologic measurements and device parameters to clinical IT systems, as well as image and multimedia content in both directions. Prototypical implementations of the derived components have proven to integrate well with systems of networked medical devices and with the CITI, effectively connecting these heterogeneous domains. Our qualitative evaluation indicates that the interoperability concepts are suitable to be integrated into clinical workflows and are expected to benefit patients and clinicians alike. The upcoming HL7 Fast Healthcare Interoperability Resources (FHIR) communication standard will likely change the domain of clinical IT significantly. A straightforward mapping to its resource model thus ensures the tenability of these concepts despite a foreseeable change in demand and requirements.
Electrochemical systems and methods using metal halide to form products (2023)

There are provided electrochemical methods and systems to form one or more organic compounds or enantiomers thereof selected from the group consisting of substituted or unsubstituted dioxane, substituted or unsubstituted dioxolane, dichloroethylether, dichloromethyl methyl ether, dichloroethyl methyl ether, chloroform, carbon tetrachloride, phosgene, and combinations thereof.
[Development of a novel influenza vaccine derived from a continuous cell line]. (2001)

Influenza viruses for production are presently produced in embryonated hen"s eggs. This conventional standard methodology is extremely cumbersome; it requires millions of eggs and an extensive purification to reduce the amount of contaminating egg proteins and to minimise the risk of allergies against egg albumin. The shortage of eggs in a pandemic situation, the selection of egg-adapted variants and the presence of adventitious viruses has emphasised the necessity for production of Influenza vaccines on a well characterised stable cell line. Our established serum and protein free Vero cell technology has been successfully adapted to large scale production of a huge variety of Influenza virus strains. The production in 1200 liter fermenter cultures under serum free conditions gave antigen yields comparable to the conventional embryonated egg technology. The development of a rapid and efficient purification scheme resulted in a safe high purity vaccine which was at least as immunogenic as conventional egg-derived vaccines in a mouse model. Clinical trials in the UK, Poland and Austria demonstrated that the Vero cell derived influenza vaccine is well tolerated, safe and highly immunogenic in humans.