MP25-08 DEVELOPMENT AND DELIVERY OF RENAL PROGENITOR ORGANOIDS FOR RAPID KIDNEY TISSUE INTEGRATION AND FUNCTIONAL RECOVERY (2021)

You have accessJournal of UrologyInfections/Inflammation/Cystic Disease of the Genitourinary Tract: Kidney & Bladder I (MP25)1 Sep 2021MP25-08 DEVELOPMENT AND DELIVERY OF RENAL PROGENITOR ORGANOIDS FOR RAPID KIDNEY TISSUE INTEGRATION AND FUNCTIONAL RECOVERY Diana Lim, Stephanie Seal, Ickhee Kim, Ji Hyun Kim, Anthony Atala, John Jackson, and James Yoo Diana LimDiana Lim More articles by this author , Stephanie SealStephanie Seal More articles by this author , Ickhee KimIckhee Kim More articles by this author , Ji Hyun KimJi Hyun Kim More articles by this author , Anthony AtalaAnthony Atala More articles by this author , John JacksonJohn Jackson More articles by this author , and James YooJames Yoo More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000002022.08AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Over 50% of patients with renal failure do not have access to the life-saving treatments of dialysis and transplantation due to a lack of transplantable organs and the high costs of maintenance care. This gap in care is predicted to grow dramatically in the next decade as the incidence of its highest causative factors, diabetes and hypertension, increases worldwide—especially in countries that lack the infrastructure for current treatments. To address this gap, we aim to develop a clinically feasible cell-based renal regeneration strategy capable of developing functional nephron structures by delivering scaffold-encapsulated progenitor-derived organoids into renal parenchyma. METHODS: We modified well-established renal organoid formation protocols using an aggrewell culture system to increase yields for enhanced reconstitution of renal structures. In vitro analyses of these organoids assessed proper differentiation and maturation. In following in vivo studies using athymic mice, we encapsulated human renal progenitor organoids in a 0.2% collagen-based scaffold and injected them into renal parenchyma. Contralateral kidneys were harvested as normal controls in addition to acellular sham controls. Assessment of morphological and biochemical parameters in vitro and in vivo demonstrated cell viability and proper upregulation of renal markers followed by renal structure formation. RESULTS: In vitro results show the expression of renal markers at 2 weeks and the formation of kidney-like structures at 4 weeks in culture. In vivo results show that acellular scaffolds alone induce tissue regeneration unseen in normal controls and the integration of implanted cells into nephron-like structures by 2 weeks in injected groups. CONCLUSIONS: These findings show that delivery of renal progenitor organoids forms renal structures and facilitates rapid kidney tissue integration in vivo. This regeneration strategy may bridge the current gaps in care in patients with renal failure. Source of Funding: Departmental funding © 2021 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e456-e456 Advertisement Copyright & Permissions© 2021 by American Urological Association Education and Research, Inc.MetricsAuthor Information Diana Lim More articles by this author Stephanie Seal More articles by this author Ickhee Kim More articles by this author Ji Hyun Kim More articles by this author Anthony Atala More articles by this author John Jackson More articles by this author James Yoo More articles by this author Expand All Advertisement PDF downloadLoading ...