gebhart/patrepeval huggingface dataset:
Three conserved consensus sequences identify the NAD‐binding site of ADP‐ribosylating enzymes, expressed by eukaryotes, bacteria and T‐even bacteriophages (1996)

It has been previously reported that the three-dimensional structures of the NAD-binding and catalytic site of bacterial toxins with ADP-ribosylating activity are superimposable, and that the key amino acids for the enzymatic activity are conserved. The model includes an NAD-binding and catalytic site formed by an alpha-helix bent over a beta-strand, surrounded by two beta-strands bearing a Glu and a His, or Arg, that are required for catalysis. We show here that the model can be extended to comprise all proteins with ADP-ribosylating activity known to date, including all eukaryotic mono- and poly-ADP-ribosyltransferases, the bacterial ADP-ribosylating enzymes which do not have toxic activity, and the analogous enzymes encoded by T-even bacteriophages. We show that, in addition to the common Glu and Arg/His amino acids previously identified, the conserved motifs can be extended as follows: (i) the Arg/His motif is usually arom-His/Arg (where 'arom' is an aromatic residue); (ii) in the sequences of the CT group the beta-strand forming part of the 'scaffold' of the catalytic cavity has an arom-ph-Ser-Thr-Ser-ph consensus (where 'ph' represents a hydrophobic residue); and (iii) the motif centered in the key glutamic residue is Glu/Gin-X-Glu; while (iv) in the sequences of the DT group the NAD-binding motif is Tyr-X10-Tyr. We believe that the model proposed not only accounts for all ADP-ribosylating proteins known to date, but it is likely to fit other enzymes (currently being analysed) which possess such an activity.
Nickel atom-olefin chemistry. Investigation of the optical spectra of binary nickel-olefin complexes (1978)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNickel atom-olefin chemistry. Investigation of the optical spectra of binary nickel-olefin complexesGeoffrey A. Ozin and William J. PowerCite this: Inorg. Chem. 1978, 17, 10, 2836–2842Publication Date (Print):October 1, 1978Publication History Published online1 May 2002Published inissue 1 October 1978https://pubs.acs.org/doi/10.1021/ic50188a031https://doi.org/10.1021/ic50188a031research-articleACS PublicationsRequest reuse permissionsArticle Views114Altmetric-Citations26LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
MASH Test 3-11 of the TxDOT Single Slope Bridge Rail (Type SSTR) on Pan-Formed Bridge Deck (2011)

The objective of this crash test was to determine whether the Texas Department of Transportation (TxDOT) Single Slope Traffic Rail (Type SSTR) would perform acceptably on a pan-formed deck when tested according to the guidelines set forth in the AASHTO Manual for Assessing Safety Hardware (MASH). The crash test performed was MASH test 3-11 involving a 2270P vehicle (5000-lb pickup truck) impacting the critical impact point (CIP) of the bridge rail at an impact speed and angle of 62 mi/h and 25 degrees, respectively. This report presents the details of the TxDOT Type SSTR on pan-formed bridge deck, description of the crash test performed, an assessment of the test results, and the implementation plan. The TxDOT Type SSTR bridge rail on pan-formed deck performed acceptably for MASH test 3-11.
A spectroelectrochemical study of copper chloro-complexes for high performance all-copper redox flow batteries (2023)

Redox Flow Batteries (RFB) are an ideal choice for large stationary applications. Among the different chemistries that can be exploited, all-copper aqueous RFB (CuRFB) use low-cost, earth-abundant raw materials with a well-defined European supply chain. The CuRFB takes advantage of the three oxidation states of copper. As Cu(I) is not stable in aqueous media, the system is based on the chlorocomplexation of the copper cations. We demonstrated that it is possible to evaluate the complexation characteristic of the concentrated solutions used in CuRFB by investigating the speciation of copper (II) in electrolytes with increasing Cu(II) concentration. Spectro-electrochemical tests in diluted solution give information on the electrochemical behavior of electrolytes with a different chloro‑complexes distribution. Quantum chemical calculations elucidate the molecular structure and electronic transitions of water solvated copper chloro‑complexes, thus complementing the experimental picture.
Pump operation monitoring applying frequency converter (2008)

Variable speed driven (VSD) pumps are nowadays widely used in industry applications. Variable speed pumping improves operational flexibility of the pump drive compared with throttle control. In many cases this results in energy cost savings. Nowadays, the performance of frequency converters allows their use as an intelligent monitoring device for the pump drive. This provides new possibilities for the maintenance and operation personnel. In this article, some functions provided by the frequency converter for pump condition and operation monitoring are explained. The applicability of a frequency converter for monitoring purposes is also discussed.
Charge Separation Effects on the Rate of Nonradiative Relaxation Processes in Quantum Dots−Quantum Well Heteronanostructures (1998)

Using time-resolved optical hole (oh)-burning techniques with femtosecond lasers, the time dependence of the spectral diffusion of the oh is examined for both the CdS quantum dot (QD) and the CdS/HgS/CdS quantum dot quantum well (QDQW) nanoparticles. It is found that the nonradiative relaxation of the optical hole is at least 3 orders of magnitude slower in the QDQW than in the QD system. Analysis of the second derivative of the broad transient bleach spectrum of the QDQW system in the 1.6−2.5 eV energy region at 50 fs delay time is found to have a minimum at 2.1 eV, corresponding to a minimum in the radiative probability. Around this energy, the rise and decay times of the transient bleach in the spectrum are found to change greatly. These results suggest that spectral diffusion in the QDQW is a result of relaxation from high- to low-energy exciton states, involving an intervening dark state at an energy of ∼2.0 eV. The energies of the maxima and minimum of the second-derivative curve are found to be in good agreement with recent theoretical calculations by Jaskolski and Bryant1 of the energies of the radiative and dark charge-separated state, respectively. In the latter, the hole is in the CdS clad and the electron is in the HgS well. The slow nonradiative relaxation processes involving this state are expected to be slow owing to the large change in the charge carrier effective masses as they cross from the CdS clad to the HgS well.
Data from Nuclear versus Cytoplasmic Localization of Filamin A in Prostate Cancer: Immunohistochemical Correlation with Metastases (2023)

&lt;div&gt;Abstract&lt;p&gt;&lt;b&gt;Purpose:&lt;/b&gt; We previously showed that nuclear localization of the actin-binding protein, filamin A (FlnA), corresponded to hormone-dependence in prostate cancer. Intact FlnA (280 kDa, cytoplasmic) cleaved to a 90 kDa fragment which translocated to the nucleus in hormone-naïve cells, whereas in hormone-refractory cells, FlnA was phosphorylated, preventing its cleavage and nuclear translocation. We have examined whether FlnA localization determines a propensity to metastasis in advanced androgen-independent prostate cancer.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Experimental Design:&lt;/b&gt; We examined, by immunohistochemistry, FlnA localization in paraffin-embedded human prostate tissue representing different stages of progression. Results were correlated with &lt;i&gt;in vitro&lt;/i&gt; studies in a cell model of prostate cancer.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Results:&lt;/b&gt; Nuclear FlnA was significantly higher in benign prostate (0.6612 ± 0.5888), prostatic intraepithelial neoplasia (PIN; 0.6024 ± 0.4620), and clinically localized cancers (0.69134 ± 0.5686) compared with metastatic prostate cancers (0.3719 ± 0.4992, &lt;i&gt;P&lt;/i&gt; = 0.0007). Cytoplasmic FlnA increased from benign prostate (0.0833 ± 0.2677), PIN (0.1409 ± 0.2293), localized cancers (0.3008 ± 0.3762, &lt;i&gt;P&lt;/i&gt; = 0.0150), to metastases (0.7632 ± 0.4414, &lt;i&gt;P&lt;/i&gt; &lt; 0.00001). Logistic regression of metastatic versus nonmetastatic tissue yielded the area under the receiver operating curve as 0.67 for nuclear-FlnA, 0.79 for cytoplasmic-FlnA, and 0.82 for both, indicating that metastasis correlates with cytoplasmic to nuclear translocation. &lt;i&gt;In vitro&lt;/i&gt; studies showed that cytoplasmic localization of FlnA induced cell invasion whereas nuclear translocation of the protein inhibited it. FlnA dephosphorylation with the protein kinase A inhibitor H-89 facilitated FlnA nuclear translocation, resulting in decreased invasiveness and AR transcriptional activity, and induced sensitivity to androgen withdrawal in hormone-refractory cells.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Conclusions:&lt;/b&gt; The data presented in this study indicate that in prostate cancer, metastasis correlates with cytoplasmic localization of FlnA and may be prevented by cleavage and subsequent nuclear translocation of this protein.&lt;/p&gt;&lt;/div&gt;
Study of organic light emitting devices with a 5,6,11,12-tetraphenylnaphthacene (rubrene)-doped hole transport layer (2002)

We investigated the stability of an organic light emitting device (OLED) with structure of indium–tin–oxide (ITO) anode/N,N′-di(naphthalene-1-yl)-N,N′-diphenyl-benzidine hole transport layer (HTL)/tris(8-hydroxyquinoline) aluminum (AlQ3) electron transport layer/Mg:Ag cathode, in which different portions of the HTL were doped with 5,6,11,12-tetraphenylnaphthacene (rubrene). Compared to undoped devices, the stability of OLEDs in which HTL doping was limited to only a thin interfacial layer at either the ITO or AlQ3 interface was essentially the same, whereas the stability of OLEDs in which a substantial portion of the HTL was doped was about an order of magnitude higher, and approached that of devices in which the whole HTL was doped. The color of the emission depended only on the material in immediate contact with AlQ3. The results demonstrate that increasing the OLED’s stability by means of doping the HTL is associated with changes in bulk HTL properties rather than interfacial properties, and is consistent with the OLED degradation mechanism based on the instability of AlQ3 cationic species.
Synthesis and structural characterization of Al-containing interlayer-expanded-MWW zeolite with high catalytic performance (2014)

Treatment of the zeolitic layered precursor of Al-MWW, so-called Al-MWW(P), with diethoxydimethylsilane (DEDMS) in acidic media leads to the formation of an aluminosilicate-type interlayer-expanded zeolite MWW (Al-IEZ-MWW) with expanded 12-membered ring (12-MR) micropores. However, the silylation process under acidic conditions simultaneously causes dealumination from the MWW framework, resulting in a decrease in the acid amount. We have developed a method for preparing Al-IEZ-MWW without leaching of the Al species. The strategy is to conduct the silylation under weakly acidic conditions; the silylation was conducted in an aqueous solution of an ammonium salt, e.g., NH4Cl, instead of HNO3. Subsequent additional acid treatment led to the formation of Al-IEZ-MWW that shows a high catalytic performance in the acylation of anisole compared to typical Al-MWW as well as Al-IEZ-MWW directly prepared under acidic conditions. The change in the state of Al atoms during the preparation process was investigated by high-resolution solid-state (27)Al MAS NMR and (27)Al MQMAS NMR techniques.
Long-Term Outcomes of Elagolix in Women With Endometriosis (2018)

ClinicalTrials.gov, NCT01760954 and NCT02143713.
Calibration of NYURay, a 3D mmWave and sub-THz Ray Tracer using Indoor, Outdoor, and Factory Channel Measurements (2023)

Ray tracing is a powerful tool that can be used to predict wireless channel characteristics, reducing the need for extensive channel measurements for channel characterization, evaluation of performance of sensing applications such as position location, and wireless network deployment. In this work, NYURay, a 3D mmWave and sub-THz ray tracer, is introduced, which is calibrated to wireless channel propagation measurements conducted at 28, 73, and 140 GHz, in indoor office, outdoor, and factory environments. We present an accurate yet low-complexity calibration procedure to obtain electrical properties of materials in any environment by modeling the reflection coefficient of building materials to be independent of the angle of incidence, a simplification shown to be quite effective in [1] over 30 years ago. We show that after calibration, NYURay can accurately predict individual directional multipath signal power. The standard deviation in the error of the directional multipath power predicted by the ray tracer compared to the directional measured power was less than 3 dB in indoor office environments and less than 2 dB in outdoor and factory environments.
SRS history and experience with palladium diffusers. Revision 1 (1995)

The Savannah River Site (SRS) has processed tritium in support of national defense programs since 1955. Palladium diffusers have been used extensively for separating hydrogen isotopes from inert gases (such as argon, helium, and nitrogen). In almost forty years of service, the design of the diffuser has been steadily improving. Several diffuser designs from different manufacturers have been evaluated at SRS. The operating experience gained from these designs together with failure analyses performed on failed units have led to several recommendations for improved diffuser designs and operating methods. This experience gained at SRS and the following recommendations form the basis of this report. Even though palladium diffuser technology has proven to be reliable, SRS has examined several alternative technologies over the past several years. This report will also review some of these promising alternatives.
Actin behavior in bulk cytoplasm is cell cycle regulated in early vertebrate embryos (2011)

The mechanical properties of cells change as they proceed through the cell cycle, primarily owing to regulation of actin and myosin II. Most models for cell mechanics focus on actomyosin in the cortex and ignore possible roles in bulk cytoplasm. We explored cell cycle regulation of bulk cytoplasmic actomyosin in Xenopus egg extracts, which is almost undiluted cytoplasm from unfertilized eggs. We observed dramatic gelation-contraction of actomyosin in mitotic (M phase) extract where Cdk1 activity is high, but not in interphase (I-phase) extract. In spread droplets, M-phase extract exhibited regular, periodic pulses of gelation-contraction a few minutes apart that continued for many minutes. Comparing actin nucleation, disassembly and myosin II activity between M-phase and I-phase extracts, we conclude that regulation of nucleation is likely to be the most important for cell cycle regulation. We then imaged F-actin in early zebrafish blastomeres using a GFP-Utrophin probe. Polymerization in bulk cytoplasm around vesicles increased dramatically during mitosis, consistent with enhanced nucleation. We conclude that F-actin polymerization in bulk cytoplasm is cell cycle regulated in early vertebrate embryos and discuss possible biological functions of this regulation.
Alumina / Aluminum Co-Continuous Ceramic Composite (C4) Materials Produced by Solid / Liquid Displacement Reactions: Processing Kinetics and Microstructures (2008)

Studies of the transformation kinetics and resulting microstructures of C materials processed at various temperatures are presented. C4 materials are produced through a displacement reaction between solid silica and molten aluminum. Evidence suggests that three distinct, temperature related processing regimes exist, each of which yields characteristic kinetic behavior and microstructures. Through the identification of the various phases and morphologies present in the microstructure, coupled with the observed kinetic behavior, a transformation mechanism for the evolution of the material is proposed.
Search for A′→μ+μ− Decays (2020)

Searches are performed for both promptlike and long-lived dark photons, A^{'}, produced in proton-proton collisions at a center-of-mass energy of 13 TeV. These searches look for A^{'}→μ^{+}μ^{-} decays using a data sample corresponding to an integrated luminosity of 5.5 fb^{-1} collected with the LHCb detector. Neither search finds evidence for a signal, and 90% confidence-level exclusion limits are placed on the γ-A^{'} kinetic mixing strength. The promptlike A^{'} search explores the mass region from near the dimuon threshold up to 70 GeV and places the most stringent constraints to date on dark photons with 214<m(A^{'})≲740 MeV and 10.6<m(A^{'})≲30 GeV. The search for long-lived A^{'}→μ^{+}μ^{-} decays places world-leading constraints on low-mass dark photons with lifetimes O(1) ps.
Modal decomposition of hopping states in cellular flames (1999)

We use Karhunen-Loeve (KL) decomposition of video images from an experiment to analyze a spatiotemporal dynamic state, unique to cellular flames, referred to as a "hopping state." Ordered states of cellular flames on a circular burner consist of one or two concentric rings of luminous cells. The hopping states correspond to the motions of individual cells in a ring sequentially executing abrupt changes in their angular position, while the other cells in the ring remain symmetric and at rest. KL decomposition separates the spatial and temporal characteristics of the hopping motion. The underlying symmetries of the experiment allow us to deduce a set of normal form equations that describe the formation of these states. We find that they result from secondary bifurcations connecting two primary branches of traveling waves. The solutions corresponding to hopping states exist as mixed-mode solutions away from the secondary bifurcations. (c) 1999 American Institute of Physics.
Newcastle disease virus, a host range-restricted virus, as a vaccine vector for intranasal immunization against emerging pathogens (2007)

The international outbreak of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) in 2002-2003 highlighted the need to develop pretested human vaccine vectors that can be used in a rapid response against newly emerging pathogens. We evaluated Newcastle disease virus (NDV), an avian paramyxovirus that is highly attenuated in primates, as a topical respiratory vaccine vector with SARS-CoV as a test pathogen. Complete recombinant NDV was engineered to express the SARS-CoV spike S glycoprotein, the viral neutralization and major protective antigen, from an added transcriptional unit. African green monkeys immunized through the respiratory tract with two doses of the vaccine developed a titer of SARS-CoV-neutralizing antibodies comparable with the robust secondary response observed in animals that have been immunized with a different experimental SARS-CoV vaccine and challenged with SARS-CoV. When animals immunized with NDV expressing S were challenged with a high dose of SARS-CoV, direct viral assay of lung tissues taken by necropsy at the peak of viral replication demonstrated a 236- or 1,102-fold (depending on the NDV vector construct) mean reduction in pulmonary SARS-CoV titer compared with control animals. NDV has the potential for further development as a pretested, highly attenuated, intranasal vector to be available for expedited vaccine development for humans, who generally lack preexisting immunity against NDV.
Electrical and Label-Free Detection of T-Cell Activation Against COVID-19: A Method Towards Point-of-Care Monitoring of Immune System (2021)

Despite great progress in biotechnology, COVID19 has revealed the inadequacy of current healthcare technologies to contain an infectious outbreak, specifically in rapid infection recognition to facilitate containment. Wide deployment of testing methods such as viral RNA (antigen) PCR and antibody detection have provided some relief in this regard; however, current technologies still require many hours to provide results. Thus, there remains a critical need for fast, sensitive, low-cost point-of-care (POC) biosensing assays to report on the infection status and immunity of patients. Emerging data on the importance of T-cell responses in generating long-term immunity against COVID19 has highlighted T-cell activation detection as a potential way for real-time monitoring native and acquired immunity. However, current approaches for T-cell stimulation monitoring are complex, laborious, time-consuming, and costly. Here we provide proof-of-principle experiments which indicate that an electrical and label-free detection method based on CMOS-compatible silicon nanowire ion-selective field-effect transistors (SNW-ISFET) can act as a fast, sensitive, reliable, and low-cost approach to monitor T-cell response to pathogens such COVID-19. This technology enables direct detection of COVID-19-specific T-cells directly from patient blood, with potential utility as a POC diagnostic device. Detection of antigen-specific T-cell activation from clonal human T-cells lines utilizing CD8-restricted peptide-MHC complexes has been demonstrated using SNW-ISFET devices. The cell concentration was varied to achieve a clear understanding about the device sensitivity, detection limit, and reliability (Figure 1). SNW-ISFET structures were applied to detect T-cell activation in clonal T-cell lines and whole peripheral blood mononuclear cell (PBMC) with multiple cell concentrations (Figure 2). Reference experiments were applied to ensure the device reliability and sensing mechanism (Figure 3). These findings show that SNW-FETs platform are promising candidates for antigen-specific T-cell activation detection from whole blood. Figure 1
Filtered multitone modulation for VDSL (2003)

We present a filter-bank modulation technique called filtered multitone (FMT) and describe its application to data transmission for very-high-speed digital subscriber line (VDSL) technology. The proposed scheme leads to significantly lower spectral overlapping between adjacent subchannels than for known multicarrier techniques such as discrete multitone (DMT) or discrete wavelet multitone. This allows mitigating interference due to echo and near-end crosstalk signals, increasing system throughput and reach, and simplifying system operations for VDSL transmission. In the receiver of an FMT system, signal equalization is accomplished in the form of per-subchannel symbol-spaced or fractionally-spaced linear or decision-feedback equalization. We study by simulation the performance that can be achieved by FMT modulation for VDSL systems where upstream and downstream signals are separated by frequency-division duplexing. We also provide comparisons with DMT modulation for a typical transmission scenario.