gebhart/patrepeval huggingface dataset:
Human Dermal Stem/Progenitor Cell-Derived Conditioned Medium Improves Senescent Human Dermal Fibroblasts (2015)

Adult skin stem cells are recognized as potential therapeutics to rejuvenate aged skin. We previously demonstrated that human dermal stem/progenitor cells (hDSPCs) with multipotent capacity could be enriched from human dermal fibroblasts using collagen type IV. However, the effects of hDSPCs on cellular senescence remain to be elucidated. In the present study, we investigated whether conditioned medium (CM) collected from hDSPC cultures (hDSPC-CM) exhibits beneficial effects on senescent fibroblasts. We found that hDSPC-CM promoted proliferation and decreased the expression level of senescence-associated β-galactosidase in senescent fibroblasts. In addition, p53 phosphorylation and p21 expression were significantly reduced in senescent fibroblasts treated with hDSPC-CM. hDSPC-CM restored the expression levels of collagen type I, collagen type III, and tissue inhibitor of metalloproteinase, and antagonized the increase of matrix metalloproteinase 1 expression. Finally, we demonstrated that hDSPC-CM significantly reduced reactive oxygen species levels by specifically up-regulating the expression level of superoxide dismutase 2. Taken together, these data suggest that hDSPC-CM can be applied as a potential therapeutic agent for improving human aged skin.
The relationship between dairy emulsion properties and the adsorbed milk protein layer: Applications in ice cream (2000)

Functional properties of dairy emulsions are closely related to the characteristics of the adsorbed layer on the dispersed droplets. Ice cream manufacture requires a mix emulsion that is stable during storage but can be destabilized by shear when frozen. A generous protein coating provides storage stability, but protein displacement by small molecule surfactants is needed to allow partial coalescence during the freezing process. An emulsion generated with a low protein surface concentration may be quiescently stable and shear unstable. Ice cream could be produced without added emulsifier by combining this emulsion (fat, fraction of protein, water) with a serum containing the remaining ingredients (remaining protein, sweeteners, stabilizers, water). The first experiment conducted was designed to optimize the centrifugation conditions (time, speed, temperature) for the determination of protein surface concentration. Next, emulsions were formed with various milk protein products (sodium caseinate, whey protein isolate, whey protein hydrolysate) at different levels and stability at rest and under shear was evaluated to identify the proper protein type and concentration to produce the desired emulsion. This emulsion was then combined with the serum phase to determine if additional protein was adsorbed, and the resulting effect on emulsion properties. Modification of the adsorbed layer (heating protein pre or post-homogenization, pH adjustment of emulsion) or serum proteins (heating, solute and polysaccharide addition) was attempted to reduce secondary adsorption and preserve emulsion functionality. Ice cream was made by the two phase process and product quality (% destabilized fat, overrun, meltdown) was compared to conventionally processed ice cream formulated with and without added emulsifier. Protein surface concentration determination was most reliable when the emulsion was centrifuged at a temperature that eliminated the solid character of the dispersed droplets. Whey protein isolate at a level of 0.3% protein was identified as the most suitable emulsifier for 25% butteroil. Combination of emulsion and serum phases led to an increase in protein surface concentration and loss of shear sensitivity unless carrageenan and sugar were included in the serum. Ice cream produced by the two-phase process compared favourably to the conventional emulsified product and was notably better than conventional product without emulsifier.
Abstract 3353: Nodal signaling mediates the aggressive, tumorigenic phenotype in breast cancer cells (2012)

Abstract The embryonic morphogen Nodal is a potent inducer of EMT and critical mediator of stem cell pluripotency in the developing embryo, but is not expressed in normal adult tissues. Intriguingly, our lab has recently shown that Nodal, a TGF-beta family member, is in fact secreted by melanoma and breast cancer cells and that Nodal expression positively correlates with tumor grade in human patient samples. Our current studies demonstrate a positive role for Nodal in regulating breast cancer cell renewal and aggressiveness. Specifically, short hairpin (shRNA) knockdown of Nodal expression in human breast cancer cell lines diminishes proliferation, invasion and clonogenicity of these cell lines in vitro and dramatically curtails tumor formation in a mouse xenograft model. Additionally, our data suggest that these effects may be caused, in part, by alterations in the expression of adhesion receptors known to function in tumor engraftment and metastasis. Taken together, our data suggest that Nodal signaling may facilitate the acquisition of self-renewal and migratory capabilities in breast cancer cells and represent a novel therapeutic target for disrupting these processes in breast cancer cells. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 3353. doi:1538-7445.AM2012-3353
Modeling, Filtering and Optimization for AFM Arrays (2012)

In this paper, we present new tools and results developed for Arrays of Microsystems and especially for Atomic Force Microscope (AFM) array design. For modeling, we developed a two-scale model of cantilever arrays in elasto dynamics. A robust optimization toolbox is interfaced to aid for design before the micro fabrication process. A model based algorithm of static state estimation using measurement of mechanical displacements by inter ferometry is stated. Quantization of inter ferometry data processing is analyzed for FPGA implementation. A robust H <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">∞</sub> filtering problem of the coupled cantilevers is solved for time invariant system with random noise effects. Our solution allows semi-decentralized computing based on functional calculus that can be implemented by networks of distributed electronic circuits as shown in a previous paper.
Comparative inference of illegitimate recombination between rice and sorghum duplicated genes produced by polyploidization (2009)

Whole-genome duplication produces massive duplicated blocks in plant genomes. Sharing appreciable sequence similarity, duplicated blocks may have been affected by illegitimate recombination. However, large-scale evaluation of illegitimate recombination in plant genomes has not been possible previously. Here, based on comparative and phylogenetic analysis of the sequenced genomes of rice and sorghum, we report evidence of extensive and long-lasting recombination between duplicated blocks. We estimated that at least 5.5% and 4.1% of rice and sorghum duplicated genes have been affected by nonreciprocal recombination (gene conversion) over nearly their full length after rice-sorghum divergence, while even more (8.7% and 8.1%, respectively) have been converted over portions of their length. We found that conversion occurs in higher frequency toward the terminal regions of chromosomes, and expression patterns of converted genes are more positively correlated than nonconverted ones. Though converted paralogs are more similar to one another than nonconverted ones, elevated nucleotide differences between rice-sorghum orthologs indicates that they have evolved at a faster rate, implying that recombination acts as an accelerating, rather than a conservative, element. The converted genes show no change in selection pressure. We also found no evidence that conversion contributed to guanine-cytosine (GC) content elevation.
An adaptive learning system yielding unbiased parameter estimates (2002)

A learning system involving model reference adaptive control (MRAC) algorithms is studied in which the Lyapunov function and its associated time derivative are simultaneous quadratic functions of both the position tracking error and the parameter estimation error. An implementation method is described which expands results of Repperger et al. (1999). Initially it appears that the condition of persistent excitation need not be explicitly satisfied, however, this condition is actually implicit in the requirements for a solution.
Supplementary Figures 1-5 and 7-8 from Dendritic Cell Internalization of α-Galactosylceramide from CD8 T Cells Induces Potent Antitumor CD8 T-cell Responses (2023)

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Chimie des processus biologiques (2019)

Enseignement Le cours n’a pas eu lieu. Recherche Le laboratoire de Chimie des processus biologiques développe des recherches à l’interface de la chimie et la biologie. Plus spécifiquement, il étudie des systèmes enzymatiques complexes impliqués dans des voies métaboliques et biosynthétiques, comme la modification des ARNs ou la biosynthèse de l’ubiquinone, du point de vue structural et mécanistique. Il s’intéresse également à certaines métalloenzymes du métabolisme bioénergétique comme les hy...
Neue Möglichkeiten der objektiven Graphitklassifizierung in Gusseisen durch Nano-Tomographie und internetbasierte Online-Verfahren (2007)

Gusseisen spielt eine wichtige Rolle als Konstruktionswerkstoff auch bei Hochleistungsanwendungen,wenn man in der Lage ist, die Ausbildung der Graphitmorphologie zu beherrschen. Dabei unterscheidet man nach DIN EN ISO 945 sechs Typen von Graphitausbildungen die in Gusseisen vorkommen. Diese unterscheiden sich nicht nur in der Form und Anordnung der jeweiligen Graphiteinlagerungen voneinander, sie differieren auch grundlegend bezüglich ihrer mechanischen und physikalischen Eigenschaften. Da die traditionelle Begutachtung der Graphitmorphologie nach Richtreihenbildern nur qualitativ ist und einen breiten subjektiven Interpretationsspielraum zulässt, erfordern Entwicklung und Einsatz von "maßgeschneiderten" Gefügen im Gusseisen auch objektive und quantifizierbare automatische Klassifikationsverfahren.\nEine 2D-Formanalyse der Graphiteinschlüsse über die konventionellen Verfahren der quantitativen Gefügeanalyse ermöglicht die Auswahl der gefügecharakteristischen Parameter für die reproduzierbare Graphitklassifizierung. Mit Hilfe von 3D-Untersuchungen der realen Graphitmorphologie mittels FIB-Nanotomographie und der Simulation möglicher 2D-Schnitte konnten erstmals die objektiven Grenzen der bildanalytischen 2D-Klassifizierung beurteilt werden. Auf dieser Basis konnten auch diejenigen bildanalytischen Messgrößen ermittelt werden, die eine Klassifizierung der Graphitmorphologie optimieren können. \nAuf dieser Basis wurde ein internetbasiertes Klassifizierungsverfahren entwickelt (www.materialography.net), das mit Hilfe des Stützvektorverfahrens diese Vielzahl bildanalytischer Messgrößen gleichzeitig in die Klassifizierung einbezieht und eine nachprüfbar hohe Klassifikationsgüte der komplexen Graphitteilchen in Gusseisen durch Angabe der Wahrscheinlichkeiten für die jeweilige Zuordnung gewährleistet.
Heat inactivation of clinical COVID-19 samples on an industrial scale for low risk and efficient high-throughput qRT-PCR diagnostic testing (2022)

We report the development of a large scale process for heat inactivation of clinical COVID-19 samples prior to laboratory processing for detection of SARS-CoV-2 by RT-qPCR. With more than 266 million confirmed cases, over 5.26 million deaths already recorded at the time of writing, COVID-19 continues to spread in many parts of the world. Consequently, mass testing for SARS-CoV-2 will remain at the forefront of the COVID-19 response and prevention for the near future. Due to biosafety considerations the standard testing process requires a significant amount of manual handling of patient samples within calibrated microbiological safety cabinets. This makes the process expensive, effects operator ergonomics and restricts testing to higher containment level laboratories. We have successfully modified the process by using industrial catering ovens for bulk heat inactivation of oropharyngeal/nasopharyngeal swab samples within their secondary containment packaging before processing in the lab to enable all subsequent activities to be performed in the open laboratory. As part of a validation process, we tested greater than 1200 clinical COVID-19 samples and showed less than 1 Cq loss in RT-qPCR test sensitivity. We also demonstrate the bulk heat inactivation protocol inactivates a murine surrogate of human SARS-CoV-2. Using bulk heat inactivation, the assay is no longer reliant on containment level 2 facilities and practices, which reduces cost, improves operator safety and ergonomics and makes the process scalable. In addition, heating as the sole method of virus inactivation is ideally suited to streamlined and more rapid workflows such as 'direct to PCR' assays that do not involve RNA extraction or chemical neutralisation methods.
Seizure Detection and Network Dynamics of Generalized Convulsive Seizures: Towards Rational Designing of Closed-Loop Neuromodulation (2017)

The preclinical study confirms thalamus as a superior target for automated detection of generalized seizures and modulation of synchrony to increase coupling may be a strategy to abate seizures.
Optimal Economic Dispatch and Power Generation for Microgrid Using Novel Lagrange Multipliers-Based Method With HIL Verification (2023)

Microgrid incorporating distributed renewable energy resources (RERs) is increasingly important owing to the goal to reach net-zero emissions by mid-century. This article deals with the optimal energy management of microgrid comprising RERs and battery energy storage system. In addition to considering power flow constraints and uncertainties of RERs, the importance of retaining profits of microgrid operators and the needs for providing extra supports to the main grid are rising. To meet all the requirements, a novel Lagrange multipliers-based method is proposed to deal with equality and inequality constraints simultaneously to directly obtain the optimal economic dispatch solution analytically. Moreover, a microgrid built in Cimei Island of Penghu Archipelago, Taiwan, is investigated to examine the compliance with the requirements of equality and inequality constraints and the performance of the Lagrange multipliers-based method. Furthermore, the comparison of the proposed method with experience-based energy management system, Newton-particle swarm optimization, and deep Q-learning network is provided to evaluate the obtained solutions. Finally, through the hardware in the loop mechanism, which is built using OPAL-RT real-time simulator with floating-point digital signal processor, the effectiveness of proposed Lagrange multipliers-based method is verified and proven to be pragmatic.
Modern ECMO: why an ECMO programme in a tertiary care hospital (2016)

Extracorporeal Membrane Oxygenation (ECMO) represents a useful tool to support the lungs and the heart when all conventional therapies failed and the patients are at risk of death. While the Extracorporeal Life Support Organization (ELSO) collects data from different institutions that joined the Registry and reports overall outcome, individual centres often collide with results below expectations, either in adults and in paediatric population. Some authors suggest that poor outcomes could be overcome with a programme dedicated to ECMO, with specialized professionals adequately trained on ECMO and with a consistent number of procedures. In 2012, The IRCCS PSD ECMO Programme was instituted with the specific aim of achieving better results than hitherto obtained. After only 1 year of activity, the results justified the programme, with a better survival rate for each group investigated, particularly in adults, but surprisingly in paediatrics too, where the results were better than what reported by ELSO. Although the number of patients treated with ECMO is still growing up, the effects of the ECMO programme continue to exert a positive action on outcome even now. The present article reports data on survival, blood loss, and blood consumption during ECMO in the last few years at our institution.
Bayesian Inference with Incomplete Knowledge Explains Perceptual Confidence and its Deviations from Accuracy (2020)

Abstract In perceptual decisions, subjects infer hidden states of the environment based on noisy sensory information. Here we show that both choice and its associated confidence are explained by a Bayesian framework based on partially observable Markov decision processes (POMDPs). We test our model on monkeys performing a direction-discrimination task with post-decision wagering, demonstrating that the model explains objective accuracy and predicts subjective confidence. Further, we show that the model replicates well-known discrepancies of confidence and accuracy, including the hard-easy effect, opposing effects of stimulus volatility on confidence and accuracy, dependence of confidence ratings on simultaneous or sequential reports of choice and confidence, apparent difference between choice and confidence sensitivity, and seemingly disproportionate influence of choice-congruent evidence on confidence. These effects may not be signatures of sub-optimal inference or discrepant computational processes for choice and confidence. Rather, they arise in Bayesian inference with incomplete knowledge of the environment.
Figure S5 from Folate Receptor Beta Designates Immunosuppressive Tumor-Associated Myeloid Cells That Can Be Reprogrammed with Folate-Targeted Drugs (2023)

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Burst synchronization on unknown frequency selective channels with co-channel interference using an antenna array (2003)

In this work, burst oriented data transmission over unknown frequency selective channels is considered. The receiver is assumed to use multiple antennas and the problem of estimating the start position of a data packet in the presence of spatially correlated co-channel interference is addressed. Each burst is assumed to contain a known training sequence, and in the paper metrics for finding the position of this training sequence are studied. More specifically, we examine the advantages of taking the spatial correlation of the co-channel interference into account, as compared with treating it as spatially white. Simulations and experimental results for transmission of normal GSM bursts in interference limited scenarios are presented.
High-Resolution OCT: An Innovative Tool for Posterior Segment Imaging (2012)

Optimal management of posterior segment disorders requires a high-resolution and preferably noninvasive imaging tool for better definition of diseases. High-resolution optical coherence tomography can provide noninvasive, high-definition imaging of the posterior segment, allowing earlier diagnosis, better follow-up of chronic cases, and more accurate and timely monitoring of the effect of therapeutic agents. Recent findings suggest an individualized approach to vitreoretinal and choroidal diseases is possible based not only on traditional ophthalmic investigations, but also on high-resolution optical coherence tomography. This innovative tool has the combined advantages of high speed, high resolution, and safe use.
Synthesis and characterization of core/shell Fe3O4/ZnSe fluorescent magnetic nanoparticles (2011)

We report on the successful preparation and characterization of fluorescent magnetic core∕shell Fe(3)O(4)∕ZnSe nanoparticles (NPs) with a spherical shape by organometallic synthesis. The 7 nm core∕3 nm shell NPs show good magnetic and photoluminescence (PL) responses. The observed PL emission∕excitation spectra are shifted to shorter wavelengths, compared to a reference ZnSe NP sample. A dramatic reduction of PL quantum yield is also observed. The temperature dependence of the magnetization for the core∕shell NPs shows the characteristic features of two coexisting and interacting magnetic (Fe(3)O(4)) and nonmagnetic (ZnSe) phases. Compared to a reference Fe(3)O(4) NP sample, the room-temperature Néel relaxation time in core∕shell NPs is three times longer.
Steady-State D3HE Proton Production in an IEC Fusion Device (2001)

Inertial electrostatic confinement of ions has been successfully used to achieve conditions necessary for the fusion of advanced fuels, such as 3He. This type of device at the University of Wisconsin was the first to produce steady-state D3He fusion, and has since produced up to 7 × 106 14.7 MeV proton/s from the D3He fusion reaction. The factors influencing the reaction rate and the experimental results are discussed.
Occult, bilateral anterior sacral and intrasacral meningeal and perineurial cysts (1990)

None of the more than 180 cases of anterior sacral meningocele reported in the past 150 years has been bilateral, and only two have been associated with occult intrasacral meningocele. We report a unique case of bilateral anterior sacral cysts, communicating with the subarachnoid space, associated with occult intrasacral meningeal and perineurial (Tarlov's) cysts, in an asymptomatic woman. The pertinent clinical and diagnostic imaging literature is reviewed.
A Comparison of Approaches for Ultra-Low-Loss Waveguides (2012)

We compare ultra-low-loss silica waveguides with PECVD SiO2, borophosphosilicate glass (BPSG), and wafer-bonded thermal oxide upper claddings. We demonstrate fiber-like (0.045 dB/m) total propagation loss in planar waveguides with bonded thermal oxide upper claddings.
DIFFERENTIAL ANTIBODY RESPONSES TO Ag-B (A REGION) AND Ia (B REGION) ANTIGENS DURING ENHANCEMENT OF RAT RENAL ALLOGRAFTS1 (1979)

Rat renal allograft survival may be enhanced by active preimmunization, or by passive transfer of antidonor antibodies. In the (LEW X BN)F1 to LEW X LEW model, antibodies to Ia-like (B region) antigens, but not Ag-B (A region) antigens promote passive enhancement. Serum antibody responses to Ia and Ag-B antigens were assessed serially in passively enhanced animals to see whether there were similar quantitative and qualitative suppressions of the immune response, as compared to unmodified rejecting controls. Acid eluates from homogenized allografts were also studied and compared to serum activities. Rejecting animals produced antibodies to both Ag-B and la gene products, rising sharply between days 4 and 7 after transplantation. Passively enhanced recipients made no detectable anti-Ia, and very weak anti-Ag-B, responses. Active immunization with cells produced anti-Ag-B and anti-Ia responses 1 week later; after transplantation there was disappearance of serum anti-Ag-B and some reduction in anti-Ia levels. When graft eluates were prepared 7 days after transplantation, passively enhanced kidneys had only traces of antibody activity, while actively enhanced kidneys had anti-Ag-B, but no anti-Ia, compared to controls in which both types of antibodies were readily recovered. Hence, antibody responses to gene products of different regions of the rat major histocompatibility complex (MHC) are not identical in states of rejection, passive, or active enhancement.
Noninvasive Estimation of Electrical Properties From Magnetic Resonance Measurements via Global Maxwell Tomography and Match Regularization (2019)

GMT could provide insight into the distribution of electromagnetic fields inside the body, which represents one of the key ongoing challenges for various diagnostic and therapeutic applications.