gebhart/patrepeval huggingface dataset:
Tel-e Vax: a genetic vaccine targeting telomerase for treatment of canine lymphoma (2019)

Introduction. Tel-eVax, a genetic vaccine targeting dog telomerase (dTERT) and based on Adenovirus (Ad)/DNA-Electro-Gene-Transfer (DNA-EGT) technology combined to COP therapy has been previously shown to induce a strong immune response and increase overall survival (OS) of dogs with multicentric Diffuse Large B-cell Lymphoma (DLBCL).&#13;\nThe objectives of this study were: 1) to clinically validate a new device for veterinary electroporation called Vet-ePoratorTM, based on CliniporatorTM technology (currently approved in Europe for electrochemotherapy applications and adapted to EGT); 2) to combine Tel-eVax with a 27-week Madison Wisconsin CHOP protocol for the treatment of DLBCL and compare OS with historical controls from the same geographical area treated with CHOP.&#13;\nMaterials and Methods. Seventeen dogs affected by DLBCL were vaccinated using two Ad vector injections (Prime phase) followed by DNA-EGT (Boost phase) by means of a Vet-ePoratorTM device and concomitantly treated with CHOP. The immune response was measured by previously described ELISA assays using a pool of peptides. The vaccinated animals were closely monitored for body weight, temperature and abnormal values in hematological parameters to monitor signs of toxicity and/or to detect indications of autoimmunity.&#13;\nResults. No significant adverse effects of Tel-eVax were observed. The OS of vaccine/CHOP animals was 64.5 weeks (range: 47.1-87.05), in line with the previous study. Sixty-seven percent of assessable dogs developed antibodies against the immunizing antigen. An indirect comparison between the 17 patients treated in this study with the dog cohort of lymphoma patients described by Wilson-Robles et al. (2017) in the same geographical area (452 and 244 days, respectively) suggests a ~ 2-fold OS increase, in line with our previous findings.&#13;\nConclusions. Tel-eVax in combination with CHOP is safe and immunogenic in lymphoma canine patients. These data confirm the therapeutic efficacy of dTERT vaccine and hold promise for the treatment of dogs affected by other cancer types.
Keypoint Transformer: Solving Joint Identification in Challenging Hands and Object Interactions for Accurate 3D Pose Estimation (2022)

We propose a robust and accurate method for estimating the 3D poses of two hands in close interaction from a single color image. This is a very challenging problem, as large occlusions and many confusions between the joints may happen. State-of-the-art methods solve this problem by regressing a heatmap for each joint, which requires solving two problems simultaneously: localizing the joints and recognizing them. In this work, we propose to separate these tasks by relying on a CNN to first localize joints as 2D keypoints, and on self-attention between the CNN features at these keypoints to associate them with the corresponding hand joint. The resulting architecture, which we call “Keypoint Transformer”, is highly efficient as it achieves state-of-the-art performance with roughly half the number of model parameters on the InterHand2.6M dataset. We also show it can be easily extended to estimate the 3D pose of an object manipulated by one or two hands with high performance. Moreover, we created a new dataset of more than 75,000 images of two hands manipulating an object fully annotated in 3D and will make it publicly available.
An intuitive error analysis for FDTD and comparison to MoM (2005)

The accuracy of the Finite-Difference Time-Domain (FDTD) algorithm is commonly treated in two ways: a local analysis of the error in a finite-difference stencil, and the numerical dispersion relation for plane-wave propagation. This article presents a simple solution error estimate that connects these two approaches. This error estimate is nearly identical to a solution error estimate for the Method of Moments, except that the FDTD error includes an additional dependence on the electrical problem size
Measurement of the lifetime of theBc+meson using theBc+→J/ψπ+decay mode (2015)

The difference in total widths between the B + c and B + mesons is measured using a data sample corresponding to an integrated luminosity of 3.0 fb -1 collected by the LHCb experiment in 7 and 8 TeV centre-of-mass energy proton-proton collisions at the LHC. Through the study of the time evolution of B + c J/ + and B + J/K + decays, the width difference is measured to be
Efficacy and safety of moxetumomab pasudotox (moxe) in adult patients (pts) with relapsed/refractory hairy cell leukemia (HCL) in relation to drug exposure, baseline disease burden, and immunogenicity. (2018)

7060 Background: Moxe is an immunotoxin targeting CD22 on B cells leading to cell death. The objectives of this analysis were to characterize the pharmacokinetics (PK) and evaluate the exposure-efficacy/safety of moxe in pts with HCL. Methods: Data from two HCL studies (Ph1: 49 pts dosed at 5, 10, 20, 30, 40 & 50 µg/kg IV; Ph 3: 80 pts dosed at 40 µg/kg IV) were pooled to develop a population PK model. The model derived Cmax and AUC were then used to evaluate the exposure-efficacy/safety relationship in the 2 studies independently due to differences in bioactivity of materials (Ph1 50 µg/kg was bioactive equivalent to Ph3 40 µg/kg). Efficacy endpoints included complete response (CR), durable CR (Ph3 only) and objective response rate (ORR). Safety parameters included hemolytic uremic syndrome (HUS), capillary leak syndrome (CLS), ≥Gr 2 increased creatinine (CRE), and Gr 3/4 adverse events (AE). Results: Moxe PK was linear from 5-50 µg/kg with PK well-described by a 1-compartment model. Day 1 clearance (CL) was higher than for later doses (22 vs 4 L/hr), attributed to CD22+ B cell depletion with repeated dosing. Ph3 pts with anti-drug antibody (ADA) titers > 10240 had ~4-fold increase in CL. High CL was associated with high baseline B cells. High baseline B cells/low PK were associated with lower response rate, but clinical benefit was still observed. In Ph3, rates of CR and durable CR were 56-58% and 50-53% in high (≥median) PK exposure group vs 31-34% and 14-17% in the low PK exposure group. In Ph1, rate of CR was 67-71% in high vs 46-50% in low PK exposure groups. Pts with ADA titer > 10240 had lower CR and durable CR, but clinical benefit was still observed. Pts with high PK exposure had higher incidence of CLS and CRE in Ph3 but not in Ph1. HUS incidence in both studies was low and was not evaluated further. Pts with high PK exposure in Ph1 had higher rates of Gr 3/4 AE. Incidence of Gr 3/4 AE in Ph3 40 µg/kg was consistent with that of Ph1 50 µg/kg. Conclusions: Moxe CL decreased with repeated dosing, consistent with B cell depletion. ADA titer > 10240 increased CL by ~4-fold. Pts with high PK exposure had better response but had a slightly higher incidence of Gr 3/4 AE. Clinical trial information: NCT01829711 and NCT00586924.
Design considerations for low-margin elastic optical networks in the nonlinear regime [Invited] (2019)

We demonstrate from a system design perspective that nonlinearity can be exploited to minimize the impact of system margins on system performance for both point-to-point links and elastic optical networks. A nonlinear interaction causes a 2 dB reduction in launch power to be reduced to &lt;0.25dB signal-to-noise ratio (SNR) penalty, and likewise, a 2 dB peak–peak (pk-pk) perturbation to the output power of an optical amplifier is reduced to &lt;0.25dB SNR penalty (for 5, 10, and 20 spans). Extending this to a gain ripple of 1 dB pk-pk with an internode spacing of 5×80km, 10×80km, and 20×80km, the penalty is 0.4 dB, 1.5 dB, and 5.1 dB, respectively, with pre-emphasis reducing this to 0.01 dB, 0.3 dB, and 1.2 dB, respectively. In elastic optical networks, we consider the nonlinear relationship among SNR, margin, and the fraction of capacity available. We consider scaling internode distances of a 9-node German scale network (DT9), such that the initial network diameter increases from 1120 km to 6720 km (six-fold scaling). We generate 1000 different topologies based on the scaled DT9 node locations to quantify the impact of margin. For the unscaled DT9 network, a 3 dB margin results in, on average, a 21% reduction in network throughput; however, when the internode spacing is increased six-fold to a continental scale network, the network throughput is reduced by 40%, on average, for the same 3 dB margin.
ChemInform Abstract: Synthesis of Monoalkenylated Crown Ethers and C‐Pivot Cryptands. (1994)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Induction of cadmium tolerance inEscherichia coliK-12 (1993)

Cadmium ions are bacteriocidal, resulting in exponential killing that starts immediately after exposure. We have shown that pretreatment with sublethal concentrations of cadmium induces tolerance. Protection against cadmium killing can also be obtained by preincubation at elevated temperatures, known to induce the heat-shock response. However, in contrast to pretreatment at elevated temperatures, exposure to sublethal cadmium concentrations does not induce thermotolerance.
Targeting of macromolecular carriers and liposomes by antibodies to myosin heavy chain (1991)

Macromolecular carriers and liposomes were covalently coupled to monoclonal antibodies against cardiac myosin heavy chain. Deferoxamine-modified polymers bound tightly with 67 Ga and 68 Ga radioisotopes. Ternary deferoxamine-polylysine antibody conjugates specifically targeted the radioisotopes to a myosin-coated microplate. Scatchard analysis revealed a high affinity of the conjugate for the target with a Kas of ≈10 8 M -1 . Liposomes that contained immobilized antimyosin antibodies were targeted specifically to the myosin-coated plate. Additional coating of these liposomes with polyethylene glycol reduced specific binding to the target in vitro. However, because of the presence of polyethylene glycol on the surface of liposomes, these liposomes had a long half-life and slowly cleared from the bloodstream after intravenous injection. These immunoliposomes showed up to 16- to 18-fold specific localization to the necrotic areas of the myocardium in rabbits with experimental infarction. antimyosin; chelating polymer; liposome; monoclonal antibody; myocardial infarction; radioimmunoimaging
Immunogenicity of idursulfase and clinical outcomes in very young patients (16 months to 7.5 years) with mucopolysaccharidosis II (Hunter syndrome) (2015)

Safety outcomes and spleen size response on idursulfase treatment appeared to be associated with genotype, not Ab status. Liver size and uGAG response on idursulfase treatment at Week 53 appeared to be associated with both neutralizing Ab status and genotype.
High Anodic-Voltage Focusing of Charge Carriers in Silicon Enables the Etching of Regularly-Arranged Submicrometer Pores at High Density and High Aspect-Ratio (2018)

The anodic dissolution of silicon in acidic electrolytes is a well-known technology enabling the silicon machining to be accurately controlled down to the micrometer scale in low-doped <i>n</i>-type silicon electrodes. Attempts to scale down this technology to the submicrometer scale has shown to be challenging, though it premises to enable the fabrication of meso and nano structures/systems that would greatly impact the fields of biosensors and nanomedicine. In this work, we report on the electrochemical etching at high anodic voltages (up to 40 V) of two-dimensional regular arrays of millions pores per square centimeter (up to 30 × 10<sup>6</sup> cm<sup>-2</sup>) with sub-micrometric diameter (down to ~860 nm), high depth (up to ~40 μm), and high aspect-ratio (up to ~45) using low-doped <i>n</i>-type silicon electrodes (resistivity 3-8 Ω cm). The use of high anodic voltages, which are over one order of magnitude higher than that commonly used in electrochemical etching of silicon, tremendously improves hole focusing at the pore tips during the etching and enables, in turn, the control of electrochemical etching of submicrometer-sized pores when spatial period reduces below 2 μm. A theoretical model allows experimental results to be interpreted in terms of an electric-field-enhanced focusing of holes at the tip apex of the pores at high anodic voltages, with respect to the pore base, which leads to a smaller curvature radius of the tip apex and enables, in turn, the etching of pore tips to be preferentially sustained over time and space.
<title>New print-based security strategy for the protection of valuable documents and products using moire intensity profiles</title> (2002)

In response to the ever increasing need for new anti- counterfeiting methods, a new integrated print-based authentication and security strategy is proposed for the protection of valuable documents and products, based on moire intensity profiles. This strategy combines the advantages of microstructure artistic screening for the halftoning of black-and-white or color images, the application of specially designed mathematical transformations on the microstructure, and the possibility of using non-standard color separation for printing the microstructured image with non-standard custom inks. Using our moire-based method, the overt (or covert) microstructured information from which the halftoned image is composed can be made clearly visible in the form of black-and-white of full-color moire intensity profiles with varying displacements, magnification rates and orientations, generating an attractive, dynamic visual effect which is very difficult to counterfeit.
Self-Condensation of N-tert-Butanesulfinyl Aldimines:  Application to the Rapid Asymmetric Synthesis of Biologically Important Amine-Containing Compounds (2004)

[reaction: see text] Highly diastereoselective intra- and intermolecular self-condensation reactions of N-tert-butanesulfinyl aldimines have been developed and applied to the rapid, asymmetric synthesis of trans-2-aminocyclopentanecarboxylic acid and the drug candidate SC-53116. Key to both syntheses is a novel microwave-assisted reaction in which N-sulfinyl aldimines are cleanly converted into nitriles in high-yielding concerted elimination processes.
Hybrid Digital Phase-Locked Loop and Moving Average Filtering Improves SNR in Spatio-Temporal Field Monitoring (2014)

Recent spatio-temporal B0 field monitoring methods utilise an array of NMR probes to measure the dynamics of the B0 field. The B0 field is usually characterised by spherical harmonic coefficients which are obtained from the phase signals (or phase coefficients) of the probe FIDs. A hybrid method is presented that uses a moving average filter in conjunction with a digital phase locked-loop filter to improve the SNR of the phase signals measured by the NMR probes. This method takes advantage of the FID SNR to reduce the phase jitter in the phase signal. It is also easy to implement for real-time applications.
Stabilizing contributions of sulfur-modified nucleotides: crystal structure of a DNA duplex with 2'-O-[2-(methoxy)ethyl]-2-thiothymidines (2005)

Substitution of oxygen atoms by sulfur at various locations in the nucleic acid framework has led to analogs such as the DNA phosphorothioates and 4'-thio RNA. The phosphorothioates are excellent mimics of DNA, exhibit increased resistance to nuclease degradation compared with the natural counterpart, and have been widely used as first-generation antisense nucleic acid analogs for applications in vitro and in vivo. The 4'-thio RNA analog exhibits significantly enhanced RNA affinity compared with RNA, and shows potential for incorporation into siRNAs. 2-Thiouridine (s2U) and 5-methyl-2-thiouridine (m5s2U) are natural nucleotide analogs. s2U in tRNA confers greater specificity of codon-anticodon interactions by discriminating more strongly between A and G compared with U. 2-Thio modification preorganizes the ribose and 2'-deoxyribose sugars for a C3'-endo conformation, and stabilizes heteroduplexes composed of modified DNA and complementary RNA. Combination of the 2-thio and sugar 2'-O-modifications has been demonstrated to boost both thermodynamic stability and nuclease resistance. Using the 2'-O-[2-(methoxy)ethyl]-2-thiothymidine (m5s2Umoe) analog, we have investigated the consequences of the replacement of the 2-oxygen by sulfur for base-pair geometry and duplex conformation. The crystal structure of the A-form DNA duplex with sequence GCGTAT*ACGC (T* = m5s2Umoe) was determined at high resolution and compared with the structure of the corresponding duplex with T* = m5Umoe. Notable changes as a result of the incorporation of sulfur concern the base-pair parameter 'opening', an improvement of stacking in the vicinity of modified nucleotides as measured by base overlap, and a van der Waals interaction between sulfur atoms from adjacent m5s2Umoe residues in the minor groove. The structural data indicate only minor adjustments in the water structure as a result of the presence of sulfur. The observed small structural perturbations combined with the favorable consequences for pairing stability and nuclease resistance (when combined with 2'-O-modification) render 2-thiouracil-modified RNA a promising candidate for applications in RNAi.
A new type of storage container for freeze‐etch specimens (1973)

SUMMARY A new storage container for freeze‐etch specimens is described which (1) dramatically reduces the loss of liquid nitrogen from the storage dewar, (2) which always sits on the bottom of the dewar and can be quickly identified and retrieved therefrom, (3) which cannot open accidentally during long term storage and (4) which can withstand continual exposure to temperature extremes. Using such containers over 300 different kinds of specimens can be readily stored in a 30 1 liquid nitrogen dewar.
Glucocorticoid-mediated induction of alpha 1-acid glycoprotein: evidence for hormone-regulated RNA processing. (1984)

We have studied the glucocorticoid-mediated accumulation of alpha 1-acid glycoprotein (AGP) in mRNA in HTC rat hepatoma cells. In contrast to the well-characterized primary response of mouse mammary tumor virus, in vitro transcription assays in isolated nuclei show that the rate of transcription of the AGP gene is high even in the absence of hormone. Despite the constitutive transcription of the AGP gene, no detectable AGP RNA can be found in either the cytoplasm or the nuclei of untreated cells. Previous experiments have shown that the glucocorticoid induction of AGP RNA requires ongoing protein synthesis. In conjunction with the present study, our data suggest that glucocorticoids stimulate accumulation of AGP RNA by inducing an RNA processing factor that allows production of stable transcripts.
Identification and characterization of a hepatic microsomal glucose transport protein. T3 of the glucose-6-phosphatase system? (1991)

A 52 kDa polypeptide in rat liver microsomes was identified as a glucose-binding protein by its ability to weakly bind cytochalasin B and by its cross-reactivity to an antibody raised against the human erythrocyte glucose transport protein. The microsomal glucose binding polypeptide was purified by affinity chromatography and an antibody was raised against it. The inhibitory effect of this antibody on rat microsomal glucose-6-phosphatase activity and on glucose transport out of microsomal vesicles indicates that this protein is a microsomal glucose transport protein.
Enhanced Uptake of Luminescent Quantum Dots by Live Cells Mediated by a Membrane-Active Peptide (2018)

The steady progress made over the past three decades in growing a variety of inorganic nanomaterials, with discreet control over their size and photophysical properties, has been exploited to develop several imaging and sensing applications. However, full integration of these materials into biology has been hampered by the complexity of delivering them into cells. In this report, we demonstrate the effectiveness of a chemically synthesized anticancer peptide to facilitate the rapid delivery of luminescent quantum dots (QDs) into live cells. We combine fluorescence imaging microscopy, flow cytometry, and specific endocytosis inhibition experiments to probe QD-peptide conjugate uptake by different cell lines. We consistently find that a sizable fraction of the internalized conjugates does not co-localize with endosomes or the nuclei. These findings are extremely promising for the potential integration of various nanomaterials into biological systems.
Chapter 20 Neuroactive Steroids in Brain and Relevance to Mood (2008)

Abstract Depression and anxiety often affect women in relation to reproductive events like menarche, premenstrual periods, post-partum and perimenopause. A prominent example of the interaction between mood, neuroactive-steroids and the GABA system is premenstrual dysphoric disorder (PMDD). Severe premenstrual negative mood symptoms occur in 3–8 % of women. Sex and stress hormones are metabolized to neuroactive steroids with effects on brain function as positive modulators of the GABA A receptor (called GABA-steroids) similar to benzodiazepines, barbiturates and alcohol. One example of a neuroactive sex steroid is allopregnanolone, and other GABA-steroids, are produced within the brain, by the adrenals at stress and from the ovary during the menstrual cycle. Animal and human studies show that benzodiazepines, barbiturates, alcohol and allopregnanolone have a bimodal effect on behavior. In high dosages or concentrations the positive GABA A receptor modulators are CNS depressants, anesthetic, and anxiolytic, whereas in certain sensitive individuals low concentrations instead of being anxiolytic cause severe anxiety, irritability, aggressiveness and depressive mood in 3–6 % of individuals, and moderate symptoms in up to 30%. Low concentrations of GABA-steroids are found endogenously during the luteal phase and induce adverse emotional reactions. In women with PMDD/ PMS this paradoxical effect of neuroactive steroids seems to provoke negative mood symptoms as tension, irritability and depression. The mechanism behind the effect is called disinhibition that acts together with tolerance development by GABA A receptor active substances. Effective treatments are inhibition of ovarian steroid production or changing the CNS response to neuroactive steroids.