gebhart/patrepeval huggingface dataset:
Functional entities: A concept to support product family and assembly system design (2002)

This paper is dedicated to the adaptation of product and assembly line design tools and methodology to product families. The main originality resides in the systematic decomposition of the family into so-called functional entities defined by the designer. The family is then considered as an assembly of entities which can be further designed and analysed separately. The paper presents the essentials on these concepts and describes their effects on subsequent design tools such as design-for-assembly, assembly planning or line layout.
Magnetomotive Displacement of the Tympanic Membrane Using Magnetic Nanoparticles: Toward Enhancement of Sound Perception (2018)

The results demonstrated here suggest the potential use of this noninvasive magnetomotive approach in future hearing aid applications. OCT can be utilized to investigate the magnetomotive dynamics of the TM, which may either enhance sound perception or magnetically induce the perception of sound without the need for acoustic speech signals.
Red blood cell quantification microfluidic chip using polyelectrolytic gel electrodes (2009)

This paper reports on a novel microfluidic chip with polyelectrolytic gel electrodes (PGEs) used to rapidly count the number of red blood cells (RBCs) in diluted whole blood. The proposed microdevice is based on the principle that the impedance across a microchannel between two PGEs varies sensitively as RBCs pass through it. The number and amplitude of impedance peaks provide the information about the number and size of RBCs, respectively. This system features a low-voltage dc detection method and non-contact condition between cells and metal electrodes. Major advantages include stable detection under varying cellular flow rate and position in the microchannel, little chance of cell damage due to high electric field gradient and no surface fouling of the metal electrodes. The performance of this PGEs-based system was evaluated in three steps. First, in order to observe the size-only dependence of the impedance signal, three different sizes of fluorescent microbeads (7.2, 10.0, and 15.0 mum; Bangs laboratories, USA) were used in the experiment. Second, the cell counting performance was evaluated by using 7.2 mum fluorescent microbeads, similar in size to RBCs, in various concentrations and comparing the results with an animal hematoanalyzer (MS 9-5; Melet schloesing laboratories, France). Finally, in human blood sample tests, intravenously collected whole blood was just diluted in a PBS without centrifuge or other pretreatments. The PGE-based system produced almost identical number of RBCs in over 800-fold diluted samples to the results from a commercialized human hematoanalyzer (HST-N402XE; Sysmex, Japan).
Localization of acoustic source via optimal beamfomer design (2015)

In localizing acoustic sources in an enclosed area, the effect of reverberation remains a difficult obstacle. With various reflections within the area, the acoustic energy map of the enclosure is becoming rather complex and spiky, so finding the correct location is often tricky and erroneous. In this paper, a new framework of an acoustic source localization system is proposed. First of all, based on a room model, the frequency response between a point in the room and a microphone can be calculated. The idea is to discretize the room and to form a map of beamformers for all discrete points. The beamformer design is formulated as a functional optimization problem, and the optimized frequency response function can be sought directly. We show that, under some mild conditions, any source location will be the global maximizer of a response function. Once the bearmforming map is constructed, the implementation in finding the source location can be done in real time. For illustration, we demonstrate how the proposed method localizes the source with three numerical examples.
Erratum: “Effects of Bottom Electrodes (Pt and IrO2) on Physical and Electrical Properties of Bi4-xLaxTi3O12Thin Film” (2002)

The above paper was double-printed both in Jpn. J. Appl. Phys. Vol. 40 (2001) pp. 5569–5571 and in pp. 6008–6011 due to a misconduct by the authors. The print in pp. 6008–6011 was officially cancelled, thus the above paper should be referred to, cited or mentioned in Jpn. J. Appl. Phys. Vol. 40 (2001) pp. 5569–5571 in the future. The authors of the above paper express a sincere apology to the readers for such a serious misprint.
GENERATION AND CHARACTERIZATION OF CONDITIONAL ALLELES FOR BMP-2 AND BMP-7 (2007)

INTRODUCTION: Bone Morphogenetic Proteins play multiple and important roles in embryonic development and homeostasis and tissue repair in adult tissues. In the adult or later stage embryos, systematic functional studies have so far been hampered by lack of mice that would allow conditional gene ablation, a prerequisite to overcome the early or complex phenotypes of straight null alleles. We present here the generation of conditional alleles for BMP-2 and BMP-7. Both molecules are highly conserved across mammalian species. Thus allele design is of prime importance, as particular care has to be taken not to create hypomorph alleles. For this reason, for targeting construct assembly we used Bacterial Homologous Recombination (BHR) on Bacterial Artificial Chromosomes (BAC), a technology that allows genome manipulation with base-pair precision and enabled us to take locus idiosyncrasies into consideration. For the generation of the conditional alleles we have adapted the classic two loxP-site strategy to BHR. Functional analysis in mice carrying these conditional alleles showed that they behave like null alleles following Cremediated recombination, making them suitable tools for in vivo studies.
Arachidonic acid inhibits myosin light chain phosphatase and sensitizes smooth muscle to calcium. (1992)

Arachidonic acid (AA) increased, at constant Ca2+, the levels of force and 20-kDa myosin light chain (MLC20) phosphorylation in permeabilized smooth muscle, and slowed relaxation and MLC20 dephosphorylation. The Ca(2+)-sensitizing effect of AA was not inhibited by inhibitors of AA metabolism (indomethacin, nordihydroguaiaretic acid, or propyl gallate), of protein kinase C (pseudopeptide) or by guanosine-5'-O-(beta-thiodiphosphate) and was abolished by oxidation of AA in air. A non-metabolizable AA analog, 5,8,11,14-eicosatetraynoic acid) also had Ca(2+)-sensitizing effects. Extensive treatment with saponin abolished the Ca(2+)-sensitizing effects of phorbol 12,13-dibutyrate and guanosine-5'-O-(gamma-thiotriphosphate), but not that of AA. A purified, oligomeric MLC20 phosphatase isolated from gizzard smooth muscle was dissociated into subunits by AA, and its activity was inhibited toward heavy meromyosin but not phosphorylase. We conclude that AA may act as a messenger-promoting protein phosphorylation through direct inhibition of the form of protein phosphatase(s) that dephosphorylate MLC20 in vivo.
Bradykinin-Potentiating Peptide-Paclitaxel Conjugate Directed at Ectopically Expressed Angiotensin-Converting Enzyme in Triple-Negative Breast Cancer (2021)

Triple-negative breast cancer (TNBC) is a heterogeneous subtype of breast cancer with poor prognosis. Here, we present a peptide-drug conjugate (PDC)─bradykinin-potentiating peptide-paclitaxel (BPP-PTX) conjugate─synthesized by conjugating BPP9a with PTX via a succinyl linker. BPP-PTX targets the angiotensin-converting enzyme (ACE) on TNBC cells. ACE was found to be ectopically expressed in two TNBC cell lines but was absent in both the receptor-positive breast cancer cell line and healthy kidney cell line. Overexpression, knockdown, and competitive inhibition experiments demonstrated ACE-mediated cytotoxicity of BPP-PTX. In vivo, ACE-positive tumors were enriched with BPP-PTX, with the PDC being better tolerated than plain PTX. Compared with plain PTX, BPP-PTX exhibited improved tumor-suppressive effects in MDA-MB-468 xenografted female nude mice. Meanwhile, BPP-PTX resulted in less body weight loss and white blood cell reduction toxicity. These results collectively imply the novelty, efficacy, and low-toxicity profile of BPP-PTX as a potential therapeutic for ACE-positive TNBC.
Incremental Benefits of a Team Approach to Reservoir Management (1996)

Teamwork is the norm for the petroleum industry where teams of geologists, geophysicists, and petroleum engineers work together to improve profits through a better understanding of reservoir size, compartmentalization, and orientation as well as reservoir flow characteristics. An integrated study of the Terry Sandstone production from the Hambert-Aristocrat Field, Weld County, Colorado was used to document the process of integration.The process of integration has proven to be iterative. This paper gives specific examples of how a multidisciplinary approach was used to improve the reservoir description and to narrow the uncertainty in the reserves estimates in the study area. The incremental benefit of narrowing the uncertainty in reserve estimates is estimated to have a NPV discounted at 10% of $20 million for the area studied. This work, done with the support of the Department of Energy, is directed to the needs of the independent oil producer.Introduction. The Hambert-Aristocrat Field in the Denver Julesburg Basin in Colorado was selected to test methods of integration. The Denver Julesburg Basin, Fig. 1, has been one of the most active in the country. The Hambert-Aristocrat Field, discovered in 1972, produces oil and gas from the Cretaceous Terry Sandstone along the east side of the Denver Basin. The Terry Sandstone in the study area produces from a depth of approximately 4500 feet, and there are approximately 101 Terry completions.Teamwork and integrated reservoir management is the current method of problem solving in the petroleum industry. A specific project goal is to demonstrate how a multidisciplinary approach can be used to detect reservoir compartmentalization and to improve the reservoir description and reserves estimates. This paper presents a discussion of the concept of multiple working hypotheses, a general strategy for integration, a final interpretation for the study area, examples of integration, and the economic benefits of reducing the uncertainty in reserves estimates.Multiple Perspectives. The challenge for the independent operators is to know when to integrate and at what level the integration should take place to make the most out of available data. Cycle time for the iterations, another consideration if profits are to be maximized as a result of the integration, are not addressed in this research.The approach espoused by T.C. Chamberlain in his classic paper
Low-N protein engineering with data-efficient deep learning (2020)

Abstract Protein engineering has enormous academic and industrial potential. However, it is limited by the lack of experimental assays that are consistent with the design goal and sufficiently high-throughput to find rare, enhanced variants. Here we introduce a machine learning-guided paradigm that can use as few as 24 functionally assayed mutant sequences to build an accurate virtual fitness landscape and screen ten million sequences via in silico directed evolution. As demonstrated in two highly dissimilar proteins, avGFP and TEM-1 β-lactamase, top candidates from a single round are diverse and as active as engineered mutants obtained from previous multi-year, high-throughput efforts. Because it distills information from both global and local sequence landscapes, our model approximates protein function even before receiving experimental data, and generalizes from only single mutations to propose high-functioning epistatically non-trivial designs. With reproducible &gt;500% improvements in activity from a single assay in a 96-well plate, we demonstrate the strongest generalization observed in machine-learning guided protein function optimization to date. Taken together, our approach enables efficient use of resource intensive high-fidelity assays without sacrificing throughput, and helps to accelerate engineered proteins into the fermenter, field, and clinic.
Best of DDW 2006. (2006)

Highlights from the 2006 Digestive Disease Week May 20-25, 2006, Los Angeles, CA. In this meeting review, many of our editorial board members report on Digestive Disease Week 2006. They highlight the most noteworthy presentations in their respective areas of expertise, including the latest treatments, technologies, and diagnostic advances in ulcerative colitis, Crohn's disease, Helicobacter pylori infection, gastroesophageal reflux disease, irritable bowel syndrome, colorectal cancer, pancreatic and biliary disease, and liver disease.
Functional Expression and Characterization of an Archaeal Aquaporin (2003)

Researchers have described aquaporin water channels from diverse eubacterial and eukaryotic species but not from the third division of life, Archaea. Methanothermobacter marburgensis is a methanogenic archaeon that thrives under anaerobic conditions at 65 degrees C. After transfer to hypertonic media, M. marburgensis sustained cytoplasmic shrinkage that could be prevented with HgCl(2). We amplified aqpM by PCR from M. marburgensis DNA. Like known aquaporins, the open reading frame of aqpM encodes two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). Unlike other known homologs, the putative Hg(2+)-sensitive cysteine was found proximal to the first NPA motif in AqpM, rather than the second. Moreover, amino acids distinguishing water-selective homologs from glycerol-transporting homologs were not conserved in AqpM. A fusion protein, 10-His-AqpM, was expressed and purified from Escherichia coli. AqpM reconstituted into proteoliposomes was shown by stopped-flow light scattering assays to have elevated osmotic water permeability (P(f) = 57 microm x s(-1) versus 12 microm x s(-1) of control liposomes) that was reversibly inhibited with HgCl(2). Transient, initial glycerol permeability was also detected. AqpM remained functional after incubations at temperatures above 80 degrees C and formed SDS-stable tetramers. Our studies of archaeal AqpM demonstrate the ubiquity of aquaporins in nature and provide new insight into protein structure and transport selectivity.
Oxygen Exchange Processes between Oxide Memristive Devices and Water Molecules (2018)

Resistive switching based on transition metal oxide memristive devices is suspected to be caused by the electric-field-driven motion and internal redistribution of oxygen vacancies. Deriving the detailed mechanistic picture of the switching process is complicated, however, by the frequently observed influence of the surrounding atmosphere. Specifically, the presence or absence of water vapor in the atmosphere has a strong impact on the switching properties, but the redox reactions between water and the active layer have yet to be clarified. To investigate the role of oxygen and water species during resistive switching in greater detail, isotope labeling experiments in a N<sub>2</sub> /H<sub>2</sub><sup>18</sup> O tracer gas atmosphere combined with time-of-flight secondary-ion mass spectrometry are used. It is explicitly demonstrated that during the RESET operation in resistive switching SrTiO<sub>3</sub> -based memristive devices, oxygen is incorporated directly from water molecules or oxygen molecules into the active layer. In humid atmospheres, the reaction pathway via water molecules predominates. These findings clearly resolve the role of humidity as both oxidizing agent and source of protonic defects during the RESET operation.
Large-scale enzymic synthesis with cofactor regeneration: glucose 6-phosphate (1977)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLarge-scale enzymic synthesis with cofactor regeneration: glucose 6-phosphateAlfred Pollak, Richard L. Baughn, and George M. WhitesidesCite this: J. Am. Chem. Soc. 1977, 99, 7, 2366–2367Publication Date (Print):March 1, 1977Publication History Published online1 May 2002Published inissue 1 March 1977https://pubs.acs.org/doi/10.1021/ja00449a071https://doi.org/10.1021/ja00449a071research-articleACS PublicationsRequest reuse permissionsArticle Views331Altmetric-Citations51LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Absolute frequency measurement of an acetylene-stabilized laser at 1542 nm (2005)

The absolute frequency of an acetylene-stabilized laser is measured using femtosecond combs based on mode-locked Ti:sapphire and fiber lasers. The acetylene-stabilized laser serves as an important optical frequency standard for telecommunication applications.
Twelve-month results of a multicenter, blinded, pilot study of a novel peptide (B2A) in promoting lumbar spine fusion (2015)

Prefix appears to provide a safe alternative to autogenous ICBG. Prefix 750 appears to show superior radiographic fusion when compared with autograft at 12 months after TLIF, although no statistically significant difference was demonstrated in this small study. Prefix and control groups both appeared to demonstrate comparable improvements to ODI at 12 months.
Fully Integrated, Miniature, High-Frequency Flow Probe Utilizing MEMS Leadless SOI Technology (2013)

This work focused on developing, fabricating, and fully calibrating a flowangle probe for aeronautics research by utilizing the latest microelectromechanical systems (MEMS), leadless silicon on insulator (SOI) sensor technology. While the concept of angle probes is not new, traditional devices had been relatively large due to fabrication constraints; often too large to resolve flow structures necessary for modern aeropropulsion measurements such as inlet flow distortions and vortices, secondary flows, etc. Mea surements of this kind demanded a new approach to probe design to achieve sizes on the order of 0.1 in. (.3 mm) diameter or smaller, and capable of meeting demanding requirements for accuracy and ruggedness. This approach invoked the use of stateof- the-art processing techniques to install SOI sensor chips directly onto the probe body, thus eliminating redundancy in sensor packaging and probe installation that have historically forced larger probe size. This also facilitated a better thermal match between the chip and its mount, improving stability and accuracy. Further, the leadless sensor technology with which the SOI sensing element is fabricated allows direct mounting and electrical interconnecting of the sensor to the probe body. This leadless technology allowed a rugged wire-out approach that is performed at the sensor length scale, thus achieving substantial sensor size reductions. The technology is inherently capable of high-frequency and high-accuracy performance in high temperatures and harsh environments.
An Effort to Improve Advanced Problem-based Learning Tutorial (2009)

To prepare for the introduction of the advanced problem-based learning (PBL) tutorial for higher-grade students under the six-year pharmacy curriculum, a trial of the tutorial was performed in a fourth-grade class under the former four-year curriculum in 2007. A questionnaire survey conducted to identify any problems in performing the tutorial revealed: 1) the number of students in each group was too large; 2) the contents of presentations seemed to overlap due to the limited number of task cases, which forced more than one group to address a particular case; and 3) the time-line from the day of product presentation to that of periodic examination was too short to hold a sufficient group discussion. In 2008, to resolve these problems: 1) the number of groups was increased to reduce the number of students in each group; 2) new task cases were added to decrease the number of groups addressing a particular case; and 3) an adequate time period was arranged between the days of product presentation and periodic examination. The survey conducted this year demonstrated that students' learning environment had been improved by these changes in the method of performing the tutorial, but also revealed new problems such as prolongation of the time required for product presentation and the difficulty levels of task cases. In addition, the usefulness of customer satisfaction (CS) analysis was demonstrated as a result of applying it to the data analysis of evaluation performed on group presentations.
Molten fuel radial motion during a high temperature transient in LWRS (1979)

Molten fuel radial motion during a high temperature transient is of interest to current light water reactor (LWR) safety analyses. Particularly, since partial or complete melting of the zircaloy cladding may occur upon the cladding being contacted by molten UO/sub 2/-fuel, because of the large difference between the melting point of the fuel and that of the cladding. During a high temperature transient, fuel melting may be initiated at the center of the rods and propagate radially outwards. Fuel cracking, which usually occurs along radial and horizontal planes in the fuel pellet during reactor normal operation provides paths for such molten fuel to relocate outside the central melting zone. This paper presents an analytical study of the transient freezing of a superheated liquid that penetrates an initially empty crack maintained at a constant subfreezing temperature. Results of the analysis are applied to assess the molten UO/sub 2/ fuel radial extrusion observed in some of the in-pile tests in which power-cooling-mismatch (PCM) conditions in a pressurized water reactor were simulated.
Detection of sulfur dioxide gas with graphene field effect transistor (2012)

Graphene grown by chemical vapor deposition on a Cu foil and transferred onto a Si wafer has been used to fabricate a field effect transistor device that was used to study the sensing of SO2 gas. It was found by in-situ measurements that the SO2 strongly p-dopes the graphene and dramatically shifts its Dirac point. This effect was used to monitor the SO2 gas. The detector can be completely reset by thermal annealing at 100 °C in high vacuum. The response and recovery of the detector are faster at higher temperatures. Moreover, the sensitivity of the SO2 graphene detector increases proportionally with increasing temperature.
Two-Year Fusion Rate Equivalency Between Grafton® DBM Gel and Autograft in Posterolateral Spine Fusion (2004)

Grafton DBM can extend a smaller quantity of autograft than is normally required to achieve a solid spinal arthrodesis. Consequently, a reduced amount of harvested autograft may be required, potentially diminishing the risk and severity of donor site complications.