gebhart/patrepeval huggingface dataset:
Bioengineering the Liver: Scale-Up and Cool Chain Delivery of the Liver Cell Biomass for Clinical Targeting in a Bioartificial Liver Support System (2012)

Acute liver failure has a high mortality unless patients receive a liver transplant; however, there are insufficient donor organs to meet the clinical need. The liver may rapidly recover from acute injury by hepatic cell regeneration given time. A bioartificial liver machine can provide temporary liver support to enable such regeneration to occur. We developed a bioartificial liver machine using human-derived liver cells encapsulated in alginate, cultured in a fluidized bed bioreactor to a level of function suitable for clinical use (performance competence). HepG2 cells were encapsulated in alginate using a JetCutter to produce ∼500 μm spherical beads containing cells at ∼1.75 million cells/mL beads. Within the beads, encapsulated cells proliferated to form compact cell spheroids (AELS) with good cell-to-cell contact and cell function, that were analyzed functionally and by gene expression at mRNA and protein levels. We established a methodology to enable a ∼34-fold increase in cell density within the AELS over 11-13 days, maintaining cell viability. Optimized nutrient and oxygen provision were numerically modeled and tested experimentally, achieving a cell density at harvest of >45 million cells/mL beads; >5×10(10) cells were produced in 1100 mL of beads. This process is scalable to human size ([0.7-1]×10(11)). A short-term storage protocol at ambient temperature was established, enabling transport from laboratory to bedside over 48 h, appropriate for clinical translation of a manufactured bioartificial liver machine.
Minimum Sensor Placement for Robust Observability of Structured Complex Networks (2015)

This paper addresses problems on the robust structural design of complex networks. More precisely, we address the problem of deploying the minimum number of dedicated sensors, i.e., those measuring a single state variable, that ensure the network to be structurally observable under disruptive scenarios. The disruptive scenarios considered are as follows: (i) the malfunction/loss of one arbitrary sensor, and (ii) the failure of connection (either unidirectional or bidirectional communication) between a pair of agents. First, we show these problems to be NP-hard, which implies that efficient algorithms to determine a solution are unlikely to exist. Secondly, we propose an intuitive two step approach: (1) we achieve an arbitrary minimum sensor placement ensuring structural observability; (2) we develop a sequential process to find minimum number of additional sensors required for robust observability. This step can be solved by recasting it as a weighted set covering problem. Although this is known to be an NP-hard problem, feasible approximations can be determined in polynomial-time that can be used to obtain feasible approximations to the robust structural design problems with optimality guarantees.
Transmission of 200Gb/s PM-16QAM and 150Gb/s PM-8QAM DWDM Signals over Long-haul and Transoceanic Distance at 100km Span Length with EDFA-only (2017)

We demonstrate transmission of 78×256Gb/s PM-16QAM over 4800km, and 78×192Gb/s PDM-8QAM over 7200km with 100km span length at spectral-efficiency 6.0 and 4.5 b/Hz/s, respectively. This is achieved by ultra-large-A <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">eff</inf> ultra-low-loss fibre using EDFA-only for amplification.
Abstract #391: HS-1200, a synthetic bile acid derivative, induces autophagy formation in human hepatoma cells (2009)

During the past several years, we have demonstrated that synthetic bile acid derivatives induce apoptosis in several human cancer cells. It is also used as mediators of cell cycle modulation in different cancer cells. In this study, we demonstrated that one of the synthetic bile acid derivatives, HS-1200, induced a strong cytotoxic effect toward HepG2 and Hep3B human hepatocellular carcinoma cells. HS-1200 showed cytotoxic effects on both cells in concentration-dependent manners. However, high doses of HS-1200 (25-100 µM) induced apoptotic cell death in both hepatoma cells, as evidenced by increases in the expressions of Bax and DNA fragmentation. On the other hand, both cells treated with low concentrations of HS-1200 (1-20 µM), which concentration range does not show apoptotic cell death in both hepatoma cells, revealed LC3-GFP aggregation of autophagocytic death. The treated hepatoma cells showed increased levels of beclin-1 and LC3-II proteins, specific markers of autophagy. The levels of Bcl-2 and p-Akt that have been implicated in the down-regulation of autophagy were decreased upon HS-1200 treatment in a concentration-dependent manner. Taken together, these findings indicate that HS-1200 induced cell death in HepG2 cells via two distinct anti-neoplastic activities of HS-1200, the ability to induce apoptosis and autophagy, therefore, suggesting that it may provide leverage to treat human liver cancer. This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD), Basic Research Promotion Fund) (KRF-2007-313-E00603). Citation Information: In: Proc Am Assoc Cancer Res; 2009 Apr 18-22; Denver, CO. Philadelphia (PA): AACR; 2009. Abstract nr 391.
Anti-Inflammatory, Antioxidant, Antibiotic, and Cytotoxic Activities of Tanacetum vulgare L. Essential Oil and Its Constituents (2017)

<b>Background:</b><i>Tanacetum vulgare</i> L. (Asteraceae) is a perennial herb that has been used to treat multiple ailments. Regional variability of the chemical composition of <i>T. vulgare</i> essential oils is well-known. Despite these regional chemotypes, most relevant studies did not analyze the complete chemical composition of the <i>T. vulgare</i> essential oil and its constituents in relation to their biological activities. Here, we assess the anti-inflammatory, antioxidant, antibacterial, and cytotoxic activities of <i>T. vulgare</i> collected from northern Quebec (Saguenay-Lac-St-Jean), Canada. <b>Methods:</b> Essential oil was extracted from plants by steam distillation and analyzed using GC-FID. Biological activities of essential oil and its main constituents were evaluated in vitro. <b>Results:</b> We identified the major compounds as camphor, borneol, and 1,8-cineole. The oil possesses anti-inflammatory activity inhibiting NO production. It also inhibits intracellular DCFH oxidation induced by tert-butylhydroperoxide. Anti-inflammatory activity of essential oil appears driven mainly by α-humulene while antioxidant activity is provided by α-pinene and caryophyllene oxide. Essential oil from <i>T vulgare</i> was active against both <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> with camphor and caryophyllene oxide responsible for antibacterial activity. Finally, <i>T. vulgare</i> essential oil was slightly cytotoxic against the human healthy cell line WS1 while α-humulene and caryophyllene oxide were moderately cytotoxic against A-549, DLD-1, and WS1. <b>Conclusion:</b> We report, for the first time, links between the specific compounds found in <i>T. vulgare</i> essential oil and anti-inflammatory, antioxidant, antibacterial, and cytotoxic activities. <i>T. vulgare</i> essential oil possesses interesting biological properties.
Análisis del comportamiento de antenas móviles multibanda en presencia del cuerpo humano (2009)

The great majority of wireless devices are used close\nto the human head. These changes the antenna performance\ncompared to free space situation. The human head absorbs an\namount of power from the antenna radiation. In this sense, it is\nimportant not only to increase the robustness of the antenna\nperformance, but also to reduce the amount of power absorbed\nby the human head. A coupled monopole and a PIFA antenna\nare evaluated in terms of antenna performance in presence of\nthe human head. The most significant parameters (SAR,\nefficiency and return losses) are obtained at two different\npositions of the antenna as a function of the frequency band, to\nfinally evaluate the impact of the human head to the antenna\nperformance, and the effect of the antenna on it. Despite both\nantennas have a similar efficiency value at Free Space,\nmonopole antenna has more sensitivity to the interaction with\nthe human head.
Mixed Labelling in Multitarget Particle Filtering (2010)

The so-called mixed labelling problem inherent to a joint state multitarget particle filter implementation is treated. The mixed labelling problem would be prohibitive for track extraction from a joint state multitarget particle filter. It is shown, using the theory of Markov chains, that the mixed labelling problem in a particle filter is inherently self-resolving. It is also shown that the factors influencing this capability are the number of particles and the number of resampling steps. Extensive quantitative analyses of these influencing factors are provided.
Scalable 11W 938nm Nd3+ doped fiber laser (2004)

Get PDF Email Share Share with Facebook Share on X Post on reddit Share with LinkedIn Add to Mendeley Add to BibSonomy Share with WeChat Get Citation Copy Citation Text J. W. Dawson, R. Beach, A. Drobshoff, Z. Liao, D. M. Pennington, S. A. Payne, L. Taylor, W. Hackenberg, and D. Bonaccini, "Scalable 11W 938nm Nd3+ doped fiber laser," in Advanced Solid-State Photonics, OSA Technical Digest (Optica Publishing Group, 2004), paper MD8. Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
Synthesis of a Walking Primitive Database for a Humanoid Robot using Optimal Control Techniques (2001)

This paper presents a general method for generating walking primitives for anthropomorphic 3D--bipeds. Corresponding control torques allowing straight ahead walking with pre--swing, swing, and heel--contact are derived by dynamic optimization using a direct collocation approach. The computed torques minimize an energy based, mixed performance index. Zero moment point (ZMP) and friction conditions at the feet ensuring postural stability of the biped, as well as bounds on the joint angles and on the control torques, are treated as constraints. The method is applied to the model of a biped with 12 joints for the purpose of developing a walking primitive database allowing straight ahead walking with situation dependent step--length adaptation. The resulting biped motions are dynamically stable and the overall motion behaviour is remarkably close to that of humans.
Measurement and theory of nondegenerate bound electronic nonlinearities in semiconductors (1991)

We recently obtained the dispersion and band-gap scaling of the bound electronic Kerr effect ( n 2 ) from a nonlinear Kramers-Kronig transformation of the nondegenerate nonlinear absorption spectrum calculated including the AC Stark, electronic Raman, and two-photon absorption effect. 1 The results for the degenerate case gave excellent agreement with the experimental results of semiconductors as well as large gap dielectrics. 2 We have extended this formalism to obtain a general expression for the nondegenerate n 2 (ω 1 ; ω 2 ) and 2 PA coefficient β(ω 1 ; ω 2 ). This allows us to calculated the change of refractive index (Δ n ) and absorption (Δ α ) at a probing frequency ω 1 , due to the presence of a pump beam at ω 2 &lt; ω g . Experiments were performed using a excite-probe (two-color) Z-scan technique. 2 , 3 Picosecond pulses from a mode-locked Nd:YAG laser at λ 2 = 1.06 μm were used as the pump beam while the second harmonic of these pulses at λ 1 = 0.532 μm probed the induced nonlinearities in two-color Z-scan geometry. Here the pump beam induces a Kerr lens in the sample, which changes the transmittance of the probe beam as transmitted through an aperture in the far field. This change is a strong function Z position (along the propagation distance) of the sample.
The Plasma Level of Interleukin-1β Can Be a Biomarker of Angiopathy in Systemic Chronic Active Epstein–Barr Virus Infection (2022)

Systemic chronic active Epstein-Barr virus infection (sCAEBV) is an EBV-positive T- or NK-cell neoplasm revealing persistent systemic inflammation. Twenty-five percent of sCAEBV patients accompany angiopathy. It is crucial to clarify the mechanisms of angiopathy development in sCAEBV because angiopathy is one of the main causes of death. Interleukin-1β (IL-1β) is reported to be involved in angiopathy onset. We investigated if IL-1β plays a role as the inducer of angiopathy of sCAEBV. We detected elevated IL-1β levels in four out of 17 sCAEBV patient's plasma. Interestingly, three out of the four had clinically associated angiopathy. None of the other patients with undetectable level of IL-1β had angiopathy. In all patients with high plasma levels of IL-1β and vascular lesions, EBV-infected cells were CD4-positive T cells. In one patient with high plasma IL-1β, the level of <i>IL-1β</i> mRNA of the monocytes was 17.2 times higher than the level of the same patient's EBV-infected cells in peripheral blood. In Ea.hy926 cells, which are the models of vascular endothelial cells, IL-1β inhibited the proliferation and induced the surface coagulation activity. IL-1β is a potent biomarker and a potent therapeutic target to treat sCAEBV accompanying angiopathy.
Serum procalcitonin as a tumoral marker in small cell lung cancer. (2011)

e17525 Background: Procalcitonin (PCT) is indicated for the early diagnosis of bacterial infections. Procalcitonin is also considered as a marker for the follow-up of thyroid carcinoma and has been found increased in patients with liver metastases. No previous study has assessed PCT in lung cancer. The aim of this study was to evaluate serum PCT as a tumoral marker for small cell lung cancer, and as a marker of liver metastasis. Methods: Sera from the Rouen University Hospital Biological Bank taken from patients that were also sampled for neurone specific enolase (NSE) were retrieved. Samples from patients with histologically proven lung cancer, taken before any treatment between December 2008 and November 2010 were selected for the study Results: From the 147 blood samples selected, 66 came from adenocarcinoma patients, 58 from neuroendocrine lung cancers (NELC) including 51 small cell lung cancers, 6 large cell lung cancers and one atypical carcinoid, 21 from squamous cell carcinomas and 2 sarcomas. Mean and median serum PCT were higher in NELC (39 ± 186 ng/ml, and 0.35 ng/ml (0.11-1.23), respectively) as compared to adecarcinoma (1.01 ± 5 and 0.08 [0.05-0.14]), or squamous cell carcinoma (0.39 ± 1.08 ng/ml and 0.1 [0.06-0.19]). From this lung cancer series, a level of 5 ng/ml PCT had a sensitivity of 18 %, and a specificity of 98 % for NELC histology. ROC under curve area for NELC was 0.764 (p < 0.001). ROC under curve area of NSE for NELC was 0.939. In lung cancer from any histology with liver metastasis, a level of 0.153 ng/ml PCT had a sensitivity of 70.4%, a specificity of 70.2% (p < 0.001), a positive and negative predictive value of 53 and 67.7%, a positive and negative likehood ratio of 2.32 and -0.002. ROC under curve area was 0.749 (p < 0.001). ROC under curve area of NSE for liver metastasis was 0.759. Conclusions: PCT as lower sensitivity and specificity than NSE for the diagnosis of NELC, and a similar increase than NSE in liver metastases. Serum PCT should not be used for the early diagnosis of bacterial infection in patients with a NELC and/or with liver metastasis.
Simultaneous electroluminescence and photoluminescence aging studies of tris(8-hydroxyquinoline) aluminum-based organic light-emitting devices (2001)

Using experimental devices which do not show electroluminescence, we have previously found that photoluminescence of tris(8-hydroxyquinoline) aluminum (AlQ3), a widely used organic electroluminescent material, decreases under prolonged transport of holes. This leads to a conclusion that AlQ3 cations are unstable, and to our proposal that the same mechanism is responsible for degradation of organic light-emitting devices (OLEDs) based on AlQ3. In this communication, we broaden our studies to include simultaneous electroluminescence and photoluminescence measurements on OLEDs containing thin AlQ3 as the emitter layer. Results show that the decrease in the electroluminescence efficiency is indeed associated with degradation of the AlQ3 in the vicinity of the hole transport layer interface.
Efficient Nanofibrous Membranes for Antibacterial Wound Dressing and UV Protection (2016)

Materials with a hierarchical structure often demonstrate superior properties with combined and even synergistic effects of multiple functions. Herein, we report the design of a new class of material with a multicompartment nanofibrous structure as a promising candidate for antibacterial wound dressing and functional textile applications. The design consists in first synthesizing nanocapsules loaded with functional payloads and subsequently embedding the nanocapsules into polymer nanofibers by using the colloid-electrospinning technique. The nanocontainer-in-nanofiber structure allows for a selective and separate loading of different functional agents with different polarities, and it offers a flexible combination of the properties of nanocontainers and nanofibers. An example of the potential for these multicompartment materials is demonstrated here, in which the synergistic antibacterial effect against E. coli K-12 and B. Subtilis combined with anti-UV property is shown.
Symmetric Control Design for Multi-Evaporator Vapor Compression Systems (2017)

Multi-evaporator vapor compression systems (ME-VCS) are inherently multi-input multi-output (MIMO) systems, often with complex, highly coupled dynamics. Thus, they require more sophisticated control schemes than traditional on-off logic, or decentralized proportional-integral controllers. Unfortunately, many MIMO control design techniques are not well suited for this problem since they require complex numerical computations that do not scale gracefully for the high-dimensional dynamics of ME-VCS systems. This paper exploits the observed similarity of the room dynamics to reduce the computational complexity of designing controllers. We use a linear matrix inequality based controller synthesis technique that exploits symmetry for designing controllers for large-scale ME-VCS systems. This controller synthesis technique was applied to an ME-VCS system with 50 rooms. Using tradition control design methods required 41 hours to synthesize a controller, while our technique designed an identical controller in less than 1 second.
Compensating Power Amplifier Distortions on Radar Signals via Waveform Design (2023)

This paper aims to study the distortion effect of Power Amplifiers (PAs) on radar waveforms in terms of Integrated Sidelobe Level in Single Input and Single Output (SISO) radar systems. To this end, we consider Memory Polynomial (MP) model as behavior of the PA which considers both non-linearity and memory distortions. Then, we consider minimizing the auto-correlation of the PA output in the baseband as a design metric for compensating the distortion effect of the PA. In this regard, we proposed an algorithm based on Coordinate Descent (CD) method to design an M-ary Phase Shift Keying (MPSK) waveform, which is a discrete phase waveform. Finally, in the numerical results, we evaluate the performance of the proposed method and compare it with Digital Predistortion (DPD) method as a conventional approach for compensating the distortion effect of PA.
Daily Ultrafiltration Results in Improved Blood Pressure Control and More Efficient Removal of Small Molecules during Hemodialysis (2012)

Volume control with daily UF results in improved BP control and, by separating the UF function from HD, adequate weekly standard Kt/V >2 can be achieved with twice weekly HD.
Homozygous JAK2 V617P Gain-of-Function Mutation Is Responsible for Constitutive JAK2/STAT5 Activation and Proliferation of HEL.92.1.7. Cell Line. (2005)

Abstract HEL (Human erythroleukemia) is a growth factor independent erythroleukemic cell line established from the bone marrow of a patient with relapsed Hodgkin disease after autologous bone marrow transplantation (Martin P &amp; Papayannopoulou T: Science1982;216:1233–1235). HEL cells display a block in differentiation at the level of common erythroid-megakaryocytic progenitor and have been commonly used as a model to study erythroid and megakaryocytic differentiation. Liu RY et al. (Blood1999;93:2369–2379) reported that constitutive activation of JAK2 tyrosine kinase in Dami/HEL cell line correlated with factor independent growth. Recently, several groups of investigators described a point mutation V617P in the autoinhibitory regulatory (JH2) domain of JAK2 tyrosine kinase in patients with myeloproliferative disorders (MPD). This acquired mutation results in constitutive activation of the JAK2/STAT5 pathway in hematopoietic cells. To date, the V617P mutation represents the most common causative genetic defect detected in patients with Philadelphia chromosome negative MPD. Interestingly, rare cases of patients with myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML) were found to have the same mutation. We tested several hematopoietic cell lines including HL-60, HEL, KG-1, K-562, TF-1, U937 and UT-7 for the presence of V617P mutation using RFLP and automatic DNA sequencing. We found the homozygous mutation V617P only in HEL cells. This cell line has a very complex karyotype suggesting that a defect of multiple oncogenes or antioncogenes could be responsible for sustained cell proliferation. We employed the JAK2 inhibitor, AG490 at concentrations ranging from 0 to 100 mM and measured proliferation of HEL and control K562 cell lines using MTT assay. We observed dose-dependent inhibition of HEL cell proliferation with low micromolar concentrations of AG490 in contrast to K562 control cell line, harboring bcr-abl fusion tyrosine kinase. IC50 was 13 mM for HEL cells and 65 mM for control K562 cells, respectively. Low concentrations of AG490 significantly decreased phosphorylation of JAK2 and STAT5 in HEL cells but not in control K562 cell line, where bcr-abl activates separately JAK2 and STAT5 pathway. Exposure of HEL cells to AG490 [0–50mM] induced apoptosis as measured by annexin V labeling in a concentration dependent fashion. No significant increase in apoptosis was detected in K562 cells using similar concentrations of the JAK2 inhibitor. Overall, these results suggest that the mechanism of proliferation of the HEL cell line is driven by constitutive activation of JAK2 tyrosine kinase secondary to V617P gain-of-function mutation. Our data provides further evidence that the JAK2/STAT5 intracellular signaling pathway is preserved in this cell line. Thus, HEL cells can serve as a model to test novel JAK2 specific inhibitors in preclinical studies.