gebhart/patrepeval huggingface dataset:
The lysine-dependent stimulation of lysine catabolism in tobacco seed requires calcium and protein phosphorylation. (1995)

The accumulation of free lysine in tobacco seed triggers the stimulation of lysine-ketoglutarate reductase, an enzyme that acts in lysine catabolism. The mechanism of lysine-ketoglutarate reductase stimulation was studied in two different systems: (1) developing seeds of wild-type plants in which the low basal lysine-ketoglutarate reductase activity can be stimulated by the exogenous addition of lysine; and (2) developing seeds of transgenic tobacco plants expressing a bacterial dihydrodipicolinate synthase in which lysine-ketoglutarate reductase activity is stimulated by endogenous lysine overproduction. In both systems, the stimulation of lysine-ketoglutarate reductase activity was significantly reduced when treated with the Ca2+ chelator EGTA. Moreover, the inhibitory effect of EGTA was overcome by the addition of Ca2+ but not Mg2+, suggesting that the lysine-dependent activation of lysine-ketoglutarate reductase requires Ca2+. This was further confirmed by a significant stimulation of lysine-ketoglutarate reductase activity following the treatment of wild-type seeds with ionomycin (an ionophore that increases Ca2+ flow into the cytoplasm). In addition, treatment of wild-type seeds with the protein phosphatase inhibitor okadaic acid triggered a significant induction in lysine-ketoglutarate reductase activity, whereas treatment of the transgenic seeds with the protein kinase inhibitor K-252a caused a significant reduction in its activity. Thus, we conclude that the stimulation of lysine-ketoglutarate reductase activity by lysine in tobacco seed operates through an intracellular signaling cascade mediated by Ca2+ and protein phosphorylation.
Vascular Permeability Drives Susceptibility to Influenza Infection in a Murine Model of Sickle Cell Disease (2017)

Sickle cell disease (SCD) is a major global health concern. Patients with SCD experience disproportionately greater morbidity and mortality in response to influenza infection than do others. Viral infection is one contributing factor for the development of Acute Chest Syndrome (ACS), a major cause of morbidity and mortality in SCD patients. We determined whether the heightened sensitivity to influenza infection could be reproduced in the two different SCD murine models to ascertain the underlying mechanisms of increased disease severity. In agreement with clinical observations, we found that both genetic and bone marrow-transplanted SCD mice had greater mortality in response to influenza infection than did wild-type animals. Despite similar initial viral titers and inflammatory responses between wild-type and SCD animals during infection, SCD mice continued to deteriorate and failed to resolve the infection, resulting in increased mortality. Histopathology of the lung tissues revealed extensive pulmonary edema and vascular damage following infection, a finding confirmed by heightened vascular permeability following virus challenge. These findings implicate the development of exacerbated pulmonary permeability following influenza challenge as the primary factor underlying heightened mortality. These studies highlight the need to focus on prevention and control strategies against influenza infection in the SCD population.
VHDL-AMS system-level simulation: behavioral antenna models and Radio-Frequency Identification case study (2004)

International audience
LTE for Public Safety Networks (2015)

This chapter describes the special characteristics of Public Safety networks and explains why LTE was selected as technology enabler for future Public Safety networks. Deployment considerations and a discussion of interworking aspects complete this section.
Aromatic polymers made by reductive polydehalogenation of oligocyclic monomers as conjugated polymers of intrinsic microporosity (C-PIMs) (2019)

Incorporation of tetrabenzohepta- or -pentafulvalene connectors into soluble, aromatic polymers results in significantly different optical spectra and intrinsic microporosity.
PBMAN: A Policy-Based Management Framework for Ambient Networks (2006)

Ambient networks aim at providing new networking solutions to highly mobile users considering a mix of current and future wireless technologies. In the last years, policy-based approaches have been proposed as an effective way of managing large networks, e.g. the IETF policy framework. In this paper, we present the design and implementation of PBMAN, a Policy-based Management Framework for Ambient Networks. PBMAN is based on a P2P infrastructure, aimed at providing scalability and self-configuration features.
Osteoclast-like cells express receptor activity modifying protein 2: application of laser capture microdissection (2005)

Receptor activity modifying proteins (RAMPs) act as receptor modulators that determine the ligand specificity of receptors for the calcitonin (CT) family. The purpose of this study was to analyze the expression of RAMPs in osteoclast-like cells using the laser capture microdissection (LCM) technique. Mouse bone marrow and spleen cells were co-cultured on a film designed for LCM. After 10 days, 250 osteoclast-like cells were captured using the LCM system. Total RNA from these cells was used to synthesize cDNA and RT-PCR analysis was performed. Osteoclast-like cells expressed CT receptor (CTR), CT receptor-like receptor (CRLR) and RAMP2, but did not express RAMP1 or RAMP3. These results indicated (1) that a pure population of osteoclast-like cells can be prepared by LCM and gene expression of this population can be analyzed by RT-PCR and (2) that RT-PCR shows that osteoclast-like cells express RAMP2, CTR and CRLR, suggesting the potential for adrenomedullin binding to osteoclast-like cells. This is the first report that osteoclast-like cells express RAMP2.
Identification and characterization of lung commensal bacteria that modulate immune response in Tuberculosis (2019)

Abstract Tuberculosis (TB) is still one of the deadliest infectious diseases in humans. As TB disease is driven by exacerbation of the immune response, promising approaches include host-targeting therapies aiming at controlling the balance between pathogen eradication and limitation of the inflammation in order to prevent immunopathology. Such immune modulation has been observed through modification of the gut-microbiota compartment with oral administration of specific bacterial strains (i.e. probiotics) in other respiratory diseases. In TB, a dysbiosis of the microbiota is observed in patients, and microbiota perturbation in mice increases susceptibility to Mycobacterium tuberculosis (Mtb). Here, we assessed whether bacterial strains isolated from the recently described lung microbiota could have a local impact on Mtb infection by protecting against immune dysfunction for the host benefit. Through intranasal administration in mice, we identified different microbiota strains isolated from mouse lungs with a strong ability to modulate the lung T cell compartment. The same in vivo approach in Mtb-infected mice revealed one strain with a remarkable capacity to diminish Mtb infection-associated lung immunopathology, accompanied by a specific immune signature characterized by an increase level of regulatory T cells sharing surprisingly T-helper 17 cell markers. Collectively, this work may contribute to the design of host-directed therapies to reduce the destructive inflammatory response in TB, without further fomenting the occurrence of multiple-drug resistant Mtb strains. It will also shed light on poorly described populations in the lungs such as the RORgt+ Foxp3+ biTregs and their interactions with the microbiota.
ChemInform Abstract: Molecular and Macromolecular Nonlinear Optical Materials. Probing Architecture/Electronic Structure/Frequency Doubling Relationships via an SCF‐LCAO MECI π Electron Formalism. (1988)

Abstract The use of perturbation theory and the PPP π electron model Hamiltonian to relate quadratic molecular optical nonlinearities to architecture and electronic structure is explored for a wide range of organic chromophores.
Freshwater and Lithium from Desalination Powered by Marine Energy Sources (2020)

To our knowledge, this paper represents an initial study of a novel concept in freshwater and lithium extraction from desalination powered off-grid by marine renewable energy sources. The project's ...
Scalable Optimal Flexibility Control of Distributed Loads in the Power Grid (2018)

The rapid penetration of uncertain and unpredictable renewable energy resources into the power grid has made generation planning and real-time power balancing a challenge, thus rendering demand side advanced control a necessity. In this work, we introduce a distributed optimization framework for load flexibility control in the grid. The proposed scheme is based on Newton iteration to provide fast convergence, and is scalable to large numbers of loads at the aggregation level. The developed optimization framework is based on interior point methods and concepts of model predictive control. It uses load-aggregator communications to solve a large-scale optimization problem in a computationally distributed manner without geometric structural assumptions.
Human fetal liver contains both zinc- and copper-rich forms of metallothionein. (1980)

Metallothionein was purified under anaerobic conditions from livers of human fetuses ranging from 19 weeks gestational age to term. Homogeneous metallothionein obtained in the absence of reducing agents from the soluble fraction of the tissue which contained 24% and 85% of the total liver copper and zinc, respectively, had less than 1 g atom of copper and about 3.1 g atoms of zinc/mol. Extraction with 1% 2-mercaptoethanol of the insoluble fraction of the tissue (containing 76% and 15% of the liver copper and zinc, respectively) yielded a metallothionein with approximately 2.5 g atoms of copper and 1.3 g atoms of zinc. When the whole tissue was extracted similarly by the procedure of Rydén and Deutsch ((1978) J. Biol. Chem. 253, 519), nearly equal proportions of the two metals were obtained in the soluble fraction from which metallothionein, with about 3.0 g atoms of zinc and 1.5 g atoms of copper, was purified. Thus, zinc- and copper-rich forms of metallothionein are differently distributed between the soluble and insoluble fractions of fetal liver. Nevertheless, the predominant metal in metallothionein of human fetal liver is zinc as is the case in the adult; significant amounts of copper are also present, consistent with the elevated quantity of this metal in the fetal tissue relative to the adult. Cadmium was not detected. Zinc is much more easily removed from the protein than is copper.
Cytoplasmic synthesis of endogenous Alu complementary DNA via reverse transcription and implications in age-related macular degeneration (2021)

<i>Alu</i> retroelements propagate via retrotransposition by hijacking long interspersed nuclear element-1 (L1) reverse transcriptase (RT) and endonuclease activities. Reverse transcription of <i>Alu</i> RNA into complementary DNA (cDNA) is presumed to occur exclusively in the nucleus at the genomic integration site. Whether <i>Alu</i> cDNA is synthesized independently of genomic integration is unknown. <i>Alu</i> RNA promotes retinal pigmented epithelium (RPE) death in geographic atrophy, an untreatable type of age-related macular degeneration. We report that <i>Alu</i> RNA-induced RPE degeneration is mediated via cytoplasmic L1-reverse-transcribed <i>Alu</i> cDNA independently of retrotransposition. <i>Alu</i> RNA did not induce cDNA production or RPE degeneration in L1-inhibited animals or human cells. <i>Alu</i> reverse transcription can be initiated in the cytoplasm via self-priming of <i>Alu</i> RNA. In four health insurance databases, use of nucleoside RT inhibitors was associated with reduced risk of developing atrophic macular degeneration (pooled adjusted hazard ratio, 0.616; 95% confidence interval, 0.493-0.770), thus identifying inhibitors of this <i>Alu</i> replication cycle shunt as potential therapies for a major cause of blindness.
Layer transfer of semiconductors and complex oxides by helium and/or hydrogen implantation and wafer bonding (2003)

Die vorliegende Dissertation behandelt die physikalischen Grundlagen und die erarbeiteten materialwissenschaftlichen Ergebnisse zur Übertragung einkristalliner dünner Schichten von Galliumarsenid und einigen komplexen Oxiden auf ausgewählte Substrate mittels Helium- und/oder Wasserstoffimplantation und Waferbonden. Als erster Schritt wird ein Wafer mit Helium- oder Wasserstoffionen implantiert. Die typische Implantationsdosis liegt bei 1x1016 bis 1x1017 cm-2. Der implantierte Wafer wird dann bei Raumtemperatur auf einen Substratwafer gebondet. Eine erste Temperaturbehandlung erhöht zunächst die Festigkeit an der Bondgrenzfläche, und bei höherer Temperatur kommt es schliesslich zum Absprengen der dünnen implantierten Oberflächenschicht des einen Wafers und damit zu deren Übertragung auf den Substratwafer. Die Blasenbildung und das Absprengens von GaAs, einkristallinen Oxiden SrTiO3, LiNbO3, LaAlO3 und transparenten polykristallinen (Pb,La)(Zr,Ti)O3 (PLZT)-Keramiken durchWasserstoff - und/oder Heliumimplantation und anschliessendes Heizen wurden untersucht. Die durch die Implantationen erzeugten Defekte und deren Entwicklung bei Temperaturbehandlungen wurden mittels Querschnitts-Transmissionelektronenmikroskopie analysiert. Besondere Implantationsbedingungen induzieren grossflächiges Abblättern anstelle von Blasenbildung nach dem Heizen ungebondeter Wafer. Dieser Effekt, der erstmalig für He-implantiertes GaAs beobachtet wurde, ist mit einer schmalen Verteilung der Platelets nach der Implantation korreliert. Die anfängliche Verteilung von Platelets und Mikrorissen, die in He- und/oder H-implantierten Materialien entsteht, ist verantwortlich für die Rissentwicklung in der gestörten Schicht. Nach der Optimierung der Implantations- und Heizbedingungen für die Blasenbildung wurde die Schichtübertragung der untersuchten Materialien durch Waferbonden und Schichtabsprengen demonstriert. Dünne Schichten von GaAs auf Si wurden durch Absprengen erhalten und auch SrTiO3-Schichten wurden auf diese Weise übertragen. Es wurde gezeigt, dass nach dem Absprengen die übertragenen Schichten einkristallin bleiben. Über den originalen Smart-CutTM Prozess hinausgehend wurde eine neue He+H - Koimplantation angewendet, die zum Absprengen von einkristallinem GaAs und SrTiO3 bei relativ niedriger Temperatur (&lt; 300°C) geeignet ist. Das Absprengen bei niedriger Temperatur ist besonders wichtig für die Schichtübertragung zwischen Materialien mit sehr verschiedenen thermischen Ausdehnungskoeffizienten sowie für prozessbehandelte Wafer, die temperaturempfndliche Bereiche enthalten.
Proteome-wide analysis of HIV-specific naive and memory CD4 + T cells in unexposed blood donors (2020)

Healthy, uninfected individuals harbor HIV-specific naive and memory CD4+ T cells, and many memory T cell epitopes are similar in sequence to peptides expressed by natural commensal bacteria, suggesting potential cross-reactivity.The preexisting HIV-1–specific T cell repertoire must influence both the immunodominance of T cells after infection and immunogenicity of vaccines. We directly compared two methods for measuring the preexisting CD4+ T cell repertoire in healthy HIV-1–negative volunteers, the HLA-peptide tetramer enrichment and T cell library technique, and show high concordance (r = 0.989). Using the library technique, we examined whether naive, central memory, and/or effector memory CD4+ T cells specific for overlapping peptides spanning the entire HIV-1 proteome were detectable in 10 HLA diverse, HIV-1–unexposed, seronegative donors. HIV-1–specific cells were detected in all donors at a mean of 55 cells/million naive cells and 38.9 and 34.1 cells/million in central and effector memory subsets. Remarkably, peptide mapping showed most epitopes recognized by naive (88%) and memory (56%) CD4+ T cells had been previously reported in natural HIV-1 infection. Furthermore, 83% of epitopes identified in preexisting memory subsets shared epitope length matches (8–12 amino acids) with human microbiome proteins, suggestive of a possible cross-reactive mechanism. These results underline the power of a proteome-wide analysis of peptide recognition by human T cells for the identification of dominant antigens and provide a baseline for optimizing HIV-1–specific helper cell responses by vaccination.
Supplementary Figure 8 from STING Ligand c-di-GMP Improves Cancer Vaccination against Metastatic Breast Cancer (2023)

&lt;p&gt;PDF file - 98K, Figure S8: The effect of c-di-GMP on CD4 and CD8 T cell populations in the lymph nodes of 4T1 tumor-bearing mice.&lt;/p&gt;
Measurements of Higgs boson production in the decay channel with a pair of $$\uptau $$ leptons in proton–proton collisions at $$\sqrt{s}=13$$ TeV (2023)

Abstract Measurements of Higgs boson production, where the Higgs boson decays into a pair of $$\uptau $$ <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:mi>τ</mml:mi> </mml:math> leptons, are presented, using a sample of proton-proton collisions collected with the CMS experiment at a center-of-mass energy of "Equation missing", corresponding to an integrated luminosity of 138 $$\,\text {fb}^{-1}$$ <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:mrow> <mml:mspace/> <mml:msup> <mml:mtext>fb</mml:mtext> <mml:mrow> <mml:mo>-</mml:mo> <mml:mn>1</mml:mn> </mml:mrow> </mml:msup> </mml:mrow> </mml:math> . Three analyses are presented. Two are targeting Higgs boson production via gluon fusion and vector boson fusion: a neural network based analysis and an analysis based on an event categorization optimized on the ratio of signal over background events. These are complemented by an analysis targeting vector boson associated Higgs boson production. Results are presented in the form of signal strengths relative to the standard model predictions and products of cross sections and branching fraction to $$\uptau $$ <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:mi>τ</mml:mi> </mml:math> leptons, in up to 16 different kinematic regions. For the simultaneous measurements of the neural network based analysis and the analysis targeting vector boson associated Higgs boson production signal strengths are found to be $$0.82\pm 0.11$$ <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:mrow> <mml:mn>0.82</mml:mn> <mml:mo>±</mml:mo> <mml:mn>0.11</mml:mn> </mml:mrow> </mml:math> for inclusive Higgs boson production, $$0.67\pm 0.19$$ <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:mrow> <mml:mn>0.67</mml:mn> <mml:mo>±</mml:mo> <mml:mn>0.19</mml:mn> </mml:mrow> </mml:math> ( $$0.81\pm 0.17$$ <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:mrow> <mml:mn>0.81</mml:mn> <mml:mo>±</mml:mo> <mml:mn>0.17</mml:mn> </mml:mrow> </mml:math> ) for the production mainly via gluon fusion (vector boson fusion), and $$1.79\pm 0.45$$ <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:mrow> <mml:mn>1.79</mml:mn> <mml:mo>±</mml:mo> <mml:mn>0.45</mml:mn> </mml:mrow> </mml:math> for vector boson associated Higgs boson production.
Application of optical microscopy as a screening technique for cellulose and lignin solvent systems (2011)

Abstract Rapid and facile screening techniques to determine the effectiveness of solvents for cellulose or biomass dissolution can advance biomass processing research. Here, we report the use of a simple optical microscopy method to screen potential cellulose and lignin solvents. The described methodology was used to screen the dissolution of cellulose and lignin in two imidazolium‐based ionic liquids (ILs), two phosphonium‐based ILs, as well as a N , N ‐dimethylacetamide/lithium chloride (DMAc/LiCl) solution in less time than other techniques. The imidazolium‐based ILs and the DMAc/LiCl were found to dissolve both cellulose and lignin. Also, it was observed that one of the phosphonium‐based ILs dissolved lignin and not cellulose, demonstrating a potential for biomass fractionation applications. © 2011 Canadian Society for Chemical Engineering
Role of protein kinase B and the MAP kinase cascade in mediating the EGF‐dependent inhibition of glycogen synthase kinase 3 in Swiss 3T3 cells1 (1999)

Epidermal growth factor (EGF), insulin-like growth factor 1 (IGF1) and phorbol myristate acetate (PMA) induce the inhibition of glycogen synthase kinase 3 (GSK3) by stimulating the phosphorylation of an N-terminal serine. Here, we show that protein kinase B (PKB) plays a key role in mediating EGF-induced inhibition of GSK3alpha and that the classical MAP kinase (MAPK) cascade has two functions in this process. Firstly, it makes a transient contribution to EGF-induced inhibition of GSK3alpha. Secondly, it shortens the duration of PKB activation and GSK3alpha inhibition. In contrast, PKB alone mediates the IGF1-induced inhibition of GSK3alpha, while the MAPK cascade mediates the inhibition of GSK3alpha by PMA.
Facile Microwave Synthesis of Hierarchical Porous Copper Oxide and Its Catalytic Activity and Kinetics for Carbon Monoxide Oxidation (2022)

The synthesis of copper oxide (CuO)-based nanomaterials has received a tremendous deal of interest in recent years. Particularly, the design and development of novel CuO structures with improved physical and chemical properties have attracted immense attention, especially for catalysis applications. We report on a rational, rapid, and surfactant-free microwave synthesis (MWS) of hierarchical porous copper oxide (HP-CuO) with a three-dimensional (3D) sponge-like topology using an MWS reactor. The activity of the microwave (MW)-synthesized HP-CuO catalysts for carbon monoxide (CO) oxidation was studied and compared to CuO prepared by the conventional heating method (CHM). Results showed that HP-CuO catalysts prepared by MWS for 10 and 30 min surpassed the CuO catalyst prepared by CHM, exhibiting <i>T</i> <sub>80</sub> of 98 and 115 °C, respectively, as compared to 185 °C of CuO prepared by CHM (T<sub>80</sub> is the temperature corresponding to 80% CO conversion). In addition, the MW-synthesized HP-CuO catalysts outperformed the CHM-synthesized CuO, achieving a 100% CO conversion at 150 °C compared to 240 °C in the case of CuO prepared by CHM. Interestingly, the HP-CuO catalyst expressed workable CO conversion kinetics with a reaction rate of c.a.35 μmol s<sup>-1</sup> g<sup>-1</sup> at 150 °C and apparent activation energy (<i>E</i> <sub>a</sub>) of 82 kJ mol<sup>-1</sup>. The HP-CuO catalyst showed excellent cycling and long-term stabilities for CO oxidation up to 4 cycles and 72 h on the stream, respectively. The enhanced catalytic activity and stability of the HP-CuO catalyst appear to result from the unique topological and structural features of HP-CuO, which were revealed by SEM, XRD, Raman, BET, TGA, XPS, and TPR techniques.