gebhart/patrepeval huggingface dataset:
Selective Survival Rescue in 15-Lipoxygenase-1-deficient Retinal Pigment Epithelial Cells by the Novel Docosahexaenoic Acid-derived Mediator, Neuroprotectin D1 (2009)

The integrity of the retinal pigment epithelial (RPE) cell is essential for the survival of rod and cone photoreceptor cells. Several stressors, including reactive oxygen species, trigger apoptotic damage in RPE cells preceded by an anti-inflammatory, pro-survival response, the formation of neuroprotectin D1 (NPD1), an oxygenation product derived from the essential omega-3 fatty acid family member docosahexaenoic acid. To define the ability of NPD1 and other endogenous novel lipid mediators in cell survival, we generated a stable knockdown human RPE (ARPE-19) cell line using short hairpin RNA to target 15-lipoxygenase-1. The 15-lipoxygenase-1-deficient cells exhibited 30% of the protein expression, and 15-lipoxygenase-2 remained unchanged, as compared with an ARPE-19 cell line control established using nonspecific short hairpin RNA transfected cells. NPD1 synthesis was stimulated by tumor necrosis factor alpha/H2O2-mediated oxidative stress in nonspecific cells (controls), whereas in silenced cells, negligible amounts of NPD1, 12(S)- and 15(S)-hydroxyeicosatetraenoic acid, and lipoxin A4 were found under these conditions. Neither control nor the deficient cells showed an increase in 15-lipoxygenase-1 protein content after 16 h of oxidative stress, suggesting that the increased activity of 15-lipoxygenase-1 is due to activation of pre-existing proteins. 15-Lipoxygenase-silenced cells also displayed an exacerbated sensitivity to oxidative stress-induced apoptosis when compared with the control cells. NPD1 selectively and potently rescued 15-lipoxygenase-silenced cells from oxidative stress-induced apoptosis. These results demonstrate that 15-lipoxygenase-1 is activated by oxidative stress in ARPE-19 cells and that NPD1 is part of an early survival signaling in RPE cells.
Rapid Kalman-Filter stabilization technique for single- and multi-detector systems (2017)

Most spectroscopic applications, especially homeland security relevant equipment relies on well-calibrated spectral data. A Kalman filter is applied to the known problem of stabilization, which means online calibration of a scintillation detector to a predefined reference peak. The method uses stochastic assumptions on the measurement uncertainties and an iterative update with new measurements. Introducing prior knowledge about the stochastic and continuously resupplying new spectral data to the filter enhances the predicted gain correction value and allows a rapid determination of this value. In multi-detector setups, the spectral alignment of data from all detectors is mandatory for performing data fusion or any further evaluations. The described Kalman technique also allows to perform this rapid cross-detector synchronization online, leading to matched up spectra at acquisition time. For a 3" ×1" sodium iodide detector, a 2" ×1" LaBr3 detector and a 1ccm CZT detector, the proposed stabilisation is tested and convergence times towards reliable gain correction values are determined. In the same setup, the cross-detector synchronisation is tested, yielding an alignment of NaI and LaBr3 within very short times.
Three-Dimensional Readout of Flash X-Ray Images of Living Sperm in Water by Atomic-Force Microscopy (1991)

The imaging of living specimens in water by x-ray microscopy can be greatly enhanced with the use of an intense flash x-ray source and sophisticated technologies for reading x-ray images. A subnanosecond [corrected] x-ray pulse from a laser-produced plasma was used to record the x-ray image of living sea urchin sperm in an x-ray resist. The resist relief was visualized at high resolution by atomic-force microscopy. Internal structure of the sperm head was evident, and the carbon density in a flagellum was estimated from the relief height.
Ultrafast nano-optics: accessing structure, function, and dynamics of matter on its natural length and time scales (2013)

Structure, function, and dynamics of matter are the result of an intricate interplay of its elementary excitations defined on femtosecond time and mesoscopic nanometer length scales. This gives rise to often complex phase behavior in soft matter as well as quantum phases in correlated electron materials with competing atomic scale short-range as well as mesoscopic nano-scale interactions. I will discuss our developments and applications of new nano-optical spectroscopies to probe those interactions providing microscopic insight into otherwise difficult to probe processes in softand correlated matter. Our approach is based on the combination of plasmonic and optical antenna concepts with ultrafast and shaped laser pulses to achieve precise control of an optical excitation on the nanometer-femtosecond scale. I will introduce the enabling principles based on new regimes of near-field light-matter interaction in terms of impedance matching and control of a quantum system.
Selection of an Ideal Coolant to Ward Off the Thermal Runaway of a Pouch Type Li-Ion Battery Module (2021)

Abstract To uphold the temperature within an acceptable range of Li-ion batteries in electric vehicles, appropriate thermo-regulation strategies should be implemented. If the temperature is increased beyond the permissible range during the charging/discharging cycle, there is a possibility of overheating and electrolyte fire, which leads to degradation of the lifecycle and capability of the cell. This research suggests the usage of nanofluids as a heat transfer medium for active thermal management. A numerical approach is employed to analyze the effectiveness of nanofluids and their impact on the temperature gradient within the battery module. The thermal performance of water and water:ethylene glycol-based nanofluid is numerically examined where the water shows better performance due to excellent thermal properties, whereas the dispersion of nanoparticles in base fluids shows a notable effect on reducing the temperature of the battery module, while a limited effect on temperature uniformity. Besides, an enhancement in performance is seen with the growth in the volume fraction of nanoparticles amid an increased pumping power at the same time. The impact of different functioning parameters such as inlet velocity, coolant temperature, and discharge rate is also analyzed for water-based nanofluids. Results indicate that with an increase in coolant velocity, alumina nanofluid can provide better uniformity and reduce the battery module temperature than the base fluid.
Pharmacokinetics and safety of a novel recombinant human von Willebrand factor manufactured with a plasma-free method: a prospective clinical trial (2013)

Safety and pharmacokinetics (PK) of recombinant von Willebrand factor (rVWF) combined at a fixed ratio with recombinant factor VIII (rFVIII) were investigated in 32 subjects with type 3 or severe type 1 von Willebrand disease (VWD) in a prospective phase 1, multicenter, randomized clinical trial. rVWF was well tolerated and no thrombotic events, inhibitors, or serious adverse events were observed. The PK of rVWF ristocetin cofactor activity, VWF antigen, and collagen-binding activity were similar to those of the comparator plasma-derived (pd) VWF-pdFVIII. In vivo cleavage of ultra-large molecular-weight rVWF multimers by ADAMTS13 (a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13; the endogenous VWF protease) and generation of characteristic satellite bands were demonstrated. In 2 subjects with specific nonneutralizing anti-VWF-binding antibodies already detectable before rVWF infusion, a reduction in VWF multimers and VWF activity was observed. Stabilization of endogenous FVIII was enhanced following post-rVWF-rFVIII infusion as shown by the difference in area under the plasma concentration curve compared with pdVWF-pdFVIII (AUC0-∞) (P < .01). These data support the concept of administering rVWF alone once a therapeutic level of endogenous FVIII is achieved.
EFFECTS OF GROWTH INHIBITION BY HYDROCORTISONE ACETATE ON VARIANCE IN CHICKENS (1970)

Traditionally genotype by environment interaction studies have dealt with changes in external environment. In this experiment an attempt was made to alter internal environment and keep external environment constant. Cockerels from each of six different commercial stocks were injected with 0,1,2 and 4 mgs hydrocortisone acetate per 100 gms body weight at 14 days of age. This type of hormonal treatment was shown to release additional variability in growth without producing any stock-treatment interaction at the level of means. The results indicate a possible new avenue for future research.
A Multiscale, Wide Field, Gigapixel Camera (2011)

Recent investigations into high pixel count imaging using multiscale optics have led to a novel optical design for a wide field, gigapixel camera. We review the mechanical design and optical performance of this imager.
Abstract SY37-02: Ligand-directed degradation of GSPT1 by a novel cereblon modulator drives potent antitumor effects (2017)

Abstract The protein cereblon is part of the CRL4-CRBN E3 ubiquitin ligase complex, and has been shown to be the molecular target for the drugs lenalidomide and pomalidomide. These drugs bind to the surface of cereblon, triggering the recruitment of substrate proteins to the ligase complex where they can be ubiquitinated and subsequently degraded by the proteosome. By this mechanism, lenalidomide and pomalidomide cause the degradation of the zinc finger transcription factors Ikaros and Aiolos, which mediate the antimyeloma activity of these compounds. Here we describe the discovery of CC-885, a novel cereblon modulator with potent and broad-spectrum antiproliferative activity against a panel of tumor cell lines. Acute myeloid leukemia (AML) cell lines and patient-derived AML cells show particular sensitivity to CC-885 treatment. CC-885 achieves this activity by causing the degradation of G1 to S phase transition 1 (GSPT1), a translation termination factor required for the release of nascent peptides from the ribosome. A crystal structure of cereblon in complex with the ligase adapter protein DDB1, as well as CC-885 and GSPT1, reveals that GSPT1 interacts with both CC-885 and the surface of cereblon. The principal molecular feature on GSPT1 that binds to cereblon is a beta-hairpin incorporating a glycine residue that docks against CC-885. Surprisingly, we found evidence that a similar molecular feature mediates Ikaros recruitment, even though there is no common structural fold or sequence homology other than the key glycine residue. We thereby define the common molecular feature, or degron, shared by the known cereblon neomorphic substrates. We further describe a novel therapeutic target, GSPT1, with promise in cancer such as AML. These results further show that cereblon-mediated protein degradation can be directed against new proteins and that this mechanism enables the targeting of multiple protein classes that may be considered undruggable with conventional approaches. Citation Format: Mary Matyskiela, Gang Lu, Takumi Ito, Barbra Pagarigan, Chin-Chu Lu, Karen Miller, Wei Fang, Nai-Yu Wang, Derek Nguyen, Jack Houston, Gilles Carmel, Tam Tran, Mariko Riley, Lyn'Al Nosaka, Gabriel Lander, Svetlana Gaidarova, Shuichan Xu, Alexander Ruchelman, Hiroshi Handa, James Carmichale, Thomas O. Daniel, Brian E. Cathers, Antonia Lopez-Girona, Philip Chamberlain. Ligand-directed degradation of GSPT1 by a novel cereblon modulator drives potent antitumor effects [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr SY37-02. doi:10.1158/1538-7445.AM2017-SY37-02
Scalable IC Platform for Smart Cameras (2005)

Smart cameras are among the emerging new fields of electronics. The points of interest are in the application areas, software and IC development. In order to reduce cost, it is worthwhile to invest in a single architecture that can be scaled for the various application areas in performance (and resulting power consumption). In this paper, we show that the combination of an SIMD (single-instruction multiple-data) processor and a general-purpose DSP is very advantageous for the image processing tasks encountered in smart cameras. While the SIMD processor gives the very high performance necessary by exploiting the inherent data parallelism found in the pixel crunching part of the algorithms, the DSP offers a friendly approach to the more complex tasks. The paper continues to motivate that SIMD processors have very convenient scaling properties in silicon, making the complete, SIMD-DSP architecture suitable for different application areas without changing the software suite. Analysis of the changes in power consumption due to scaling shows that for typical image processing tasks, it is beneficial to scale the SIMD processor to use the maximum level of parallelism available in the algorithm if the IC supply voltage can be lowered. If silicon cost is of importance, the parallelism of the processor should be scaled to just reach the desired performance given the speed of the silicon.
Transcriptional Organization of the Avian Adenovirus CELO (1998)

A detailed map of the transcriptional organization of the CELO virus genome was produced. Recent computer analysis of CELO virus has indicated the presence of 38 putative open reading frames (ORFs). This study, based on analysis of the transcriptional products of CELO in vitro, confirmed the presence of RNAs for 26 of these 38 ORFs. All of the results were obtained by cDNA isolation or specific reverse transcriptase PCR. Observation of ORF transcription kinetics postinfection revealed the existence of early and late expression, with the earliest starting at 2 h postinfection. The 5' untranslated regions of some RNAs were also studied, and this revealed the existence of a bipartite leader sequence, comparable in structure to the tripartite leader of mastadenovirus. The leader most probably involved in transcriptional activity was observed in most of the structural protein genes of the CELO virus genome. This suggests some homology in transcriptional organization between the avian adenovirus CELO and known mastadenoviruses such as human adenovirus.
Evaluation of influence of planting date on growth, artemisinin yield and seed yield ofArtemisia annuaunder Bangalore agro-climatic conditions (2009)

Abstract Artemisia annua is an important crop grown for producing the anti-malarial drug artemisinin. It has been recently introduced to Bangalore's agro-climatic conditions of South India. A field experiment was carried out at the Central Institute of Medicinal and Aromatic Plants (CIMAP), Bangalore, from November 2004 to July 2007 to optimize the planting date for maximum herb, artemisinin and seed yield under the semi-arid tropical conditions of Bangalore. The study revealed that it was best to transplant seedlings of A. annua in the later part of October to get optimum herb, artemisinin and seed yield under Bangalore's agro-climatic conditions. Keywords: Artemisia annua semi-arid tropicsplanting dategrowth parameter and artemisinin yield Acknowledgements The authors would like to thank the Director, CIMAP, Lucknow, and the Council of Scientific and Industrial Research, New Delhi (India) who supported this study by providing funds and facilities.
Chloroplast Genetic Engineering for Enhanced Agronomic Traits and Expression of Proteins for Medical/Industrial Applications (2017)

Abstract The sections in this article are Introduction Historical Aspects Unique Features of Chloroplast Genetic Engineering Maternal Inheritance and Gene Containment Crop Species Stably Transformed via the Plastid Genome Agronomic Traits Conferred via the Plastid Genome Transgenic Plastids as Bioreactors Biomaterials, Enzymes, and Amino Acids Conclusions Acknowledgements
A dq-axis framework for electrostatic synchronous machines and charge oriented control (2017)

Recently, innovations such as advanced dielectric liquids and additive manufacturing have enabled electrostatic machines at the fractional horsepower scale that have competitive performance with their conventional electromagnetic counterparts. The emergence highlights a need for circuit modeling that guides machine design and drive controls, rooted in the canon of well-established electromagnetic machinery practices. This paper combines prior approaches and expands upon them to form a unified electrostatic machine dq-axis framework capable of providing insight on the capacitances of interest for torque production as well as control. Maximum torque per volt and charge oriented control are presented with a torque equation whose terms are parsed into field, elastance, and induction torque mechanisms. Complex vector voltage regulators form the heart of drive controls. The model is confirmed with finite element analysis (FEA).
Evaluation of computer-assisted mandibular reconstruction with vascularized iliac crest bone graft compared to conventional surgery: a randomized prospective clinical trial (2014)

DRKS00005181.
Supplementary Figures from Tumor-Reactive CD8<sup>+</sup> T Cells in Metastatic Gastrointestinal Cancer Refractory to Chemotherapy (2023)

&lt;p&gt;PDF file 535K, Supp. Fig. S1 shows the immunocytochemical characterization of the new gastrointestinal cancer cell lines. Suppl. Fig. S2 show the loss of reactivity of Pt.9 CD8+ TIL to autologous cancer cell line by the 6th passage&lt;/p&gt;
Technical Report: Energy Evaluation of preamble Sampling MAC Protocols\n for Wireless Sensor Networks (2011)

The paper presents a simple probabilistic analysis of the energy consumption\nin preamble sampling MAC protocols. We validate the analytical results with\nsimulations. We compare the classical MAC protocols (B-MAC and X-MAC) with\nLAMAC, a method proposed in a companion paper. Our analysis highlights the\nenergy savings achievable with LA-MAC with respect to B-MAC and X-MAC. It also\nshows that LA-MAC provides the best performance in the considered case of high\ndensity networks under traffic congestion.\n
Human Embryonic and Induced Pluripotent Stem Cell–Derived Cardiomyocytes Exhibit Beat Rate Variability and Power-Law Behavior (2012)

This is the first study demonstrating that hESC-CMs and iPSC-CMs exhibit beat rate variability and power-law behavior as in humans, thus supporting the potential capability of these cell sources to serve as biological pacemakers. Our ability to generate sinoatrial-compatible spontaneous cardiomyocytes from the patient's own hair (via keratinocyte-derived iPSCs), thus eliminating the critical need for immunosuppression, renders these myocytes an attractive cell source as biological pacemakers.
Swept Source Optical Coherence Tomography and Technology Trends (2014)

Along with an introduction to OCT technology and applications, we report a broad range of swept-source performances based on a highly-flexible external-cavity laser architecture embodied within a compact butterfly package. Multiple spectral regions (800nm to 1600nm) and sweep frequencies (1kHz to 150kHz) are demonstrated.
Cell cycle and apoptosis regulatory protein-1: a novel regulator of apoptosis in the colonic mucosa during aging (2007)

Although the regulatory mechanisms for the age-related rise in proliferation and reduction in apoptosis in the colonic mucosa are yet to be fully delineated, we have demonstrated that these events are associated with increased expression and activation of epithelial growth factor receptor (EGFR)/ErbB-1 and some of its receptor family members (EGFRs), indicating their involvement in these processes. However, the downstream signaling events of EGFR and/or its family members regulating age-related changes in mucosal proliferation and apoptosis remain to be delineated. Cell cycle and apoptosis regulatory protein-1 (CARP-1), a novel growth signaling regulator that we isolated, participates in EGFR-dependent signaling. In the current investigation, we examined the involvement of CARP-1 in colonic mucosal growth-related processes during aging. We report that the age-related reduction in apoptosis in the colonic mucosa is associated with increased expression and tyrosine phosphorylation of not only EGFR but also ErbB-2 and ErbB-3. In contrast, protein and mRNA levels of CARP-1 as well as tyrosine phosphorylation of CARP-1 are decreased. Additionally, we have observed that administration of wortmannin, an inhibitor of phosphatidylinositol 3-kinase activity that accelerates apoptosis in the colonic mucosa of aged rats, causes a marked increase in expression and tyrosine phosphorylation of CARP-1. The age-related decline in CARP-1 expression could partly be attributed to increased methylation of the CARP-1 promoter. Taken together, our data suggest that not only EGFR but also its other members are involved in regulating colonic mucosal growth during aging and that CARP-1 may play a crucial role in transducing EGFRs signals.
Calculation of modified capacity factor in micellar electrokinetic chromatography (1996)

In addition to the expression k' = (tm-t(o))/t(o) (1-tm/tmc), we propose the expression k" = (tm-t(o))/(tmc-t(o)) to calculate the capacity factor in micellar electrokinetic chromatography (MEKC), where tm, t(o), and tmc are the migration time of the analyte, the flow marker, and the micelles, respectively. The k' and k" values that were obtained from simulated data as well as from MEKC analysis of different peptides (in 100 mM sodium dodecyl sulfate/0.1 N sodium borate buffer at pH 11.0) were calculated and compared. The k" value is equal to zero for an analyte remaining in the aqueous phase whereas it is equal to one for an analyte always staying in the micellar phase. By applying k" a finite capacity factor can be obtained for an analyte, indicating its partition between the two moving phases (aqueous and micellar) even in those cases when tm equals tmc. The slope of the curve k" as a function of tm is constant through the whole migration window and therefore peak compression does not occur when applying k" to calculate the capacity factor. A given difference in k" corresponds the same difference in migration times and this value does not depend on the position within the migration window. Since k" is a normalized parameter it is easy to evaluate the significance of a given difference in capacity factor or to estimate the relative position of an analyte with a given capacity factor in the migration window by applying k". Therefore, k" seems to be an adequate parameter to calculate the capacity factor in MEKC and, similar to K', it also refers to the hydrophobicity of the analyte.
A Network Coding Approach to Loss Tomography (2012)

Network tomography aims at inferring internal network characteristics based on measurements at the edge of the network. In loss tomography, in particular, the characteristic of interest is the loss rate of individual links and multicast and/or unicast end-to-end probes are typically used. Independently, recent advances in network coding have shown that there are advantages from allowing intermediate nodes to process and combine, in addition to just forward, packets. In this paper, we study the problem of loss tomography in networks with network coding capabilities. We design a framework for estimating link loss rates, which leverages network coding capabilities, and we show that it improves several aspects of tomography, including the identifiability of links, the trade-off between estimation accuracy and bandwidth efficiency, and the complexity of probe path selection. We discuss the cases of inferring link loss rates in a tree topology and in a general topology. In the latter case, the benefits of our approach are even more pronounced compared to standard techniques but we also face novel challenges, such as dealing with cycles and multiple paths between sources and receivers. Overall, this work makes the connection between active network tomography and network coding.