gebhart/patrepeval huggingface dataset:
GLUT1-mediated glucose uptake plays a crucial role during Plasmodium hepatic infection (2016)

Intracellular pathogens have evolved mechanisms to ensure their survival and development inside their host cells. Here, we show that glucose is a pivotal modulator of hepatic infection by the rodent malaria parasite Plasmodium berghei and that glucose uptake via the GLUT1 transporter is specifically enhanced in P. berghei-infected cells. We further show that ATP levels of cells containing developing parasites are decreased, which is known to enhance membrane GLUT1 activity. In addition, GLUT1 molecules are translocated to the membrane of the hepatic cell, increasing glucose uptake at later stages of infection. Chemical inhibition of GLUT1 activity leads to a decrease in glucose uptake and the consequent impairment of hepatic infection, both in vitro and in vivo. Our results reveal that changes in GLUT1 conformation and cellular localization seem to be part of an adaptive host response to maintain adequate cellular nutrition and energy levels, ensuring host cell survival and supporting P. berghei hepatic development.
Midbrain dopaminergic neurons utilize nitric oxide/cyclic GMP signaling to recruit ERK that links retinoic acid receptor stimulation to up-regulation of BDNF (2010)

Stimulation of retinoic acid receptors (RARs) protects midbrain dopaminergic neurons, presumably via up-regulation of brain-derived neurotrophic factor (BDNF) expression. The present study was focused on unexplored signaling mechanisms linking RAR stimulation to BDNF expression. Rat midbrain slice cultures treated with an RAR agonist Am80 showed increased tissue levels of BDNF mRNA and protein as compared to cultures without treatment. Am80-induced increase in BDNF expression was observed in dopaminergic neurons, which was blocked by inhibition of extracellular signal-regulated kinase (ERK) activation. We also found that Am80 increased neuronal nitric oxide synthase expression in dopaminergic neurons even during ERK inhibition, and this increase was accompanied by 8-nitro-cyclic GMP formation. Notably, the effect of Am80 on BDNF expression was attenuated by inhibitors of nitric oxide synthase, soluble guanylyl cyclase and cyclic GMP-dependent protein kinase (PKG). Am80-induced ERK phosphorylation in dopaminergic neurons was also attenuated by inhibition of soluble guanylyl cyclase and PKG. Moreover, 8-Br-cyclic GMP induced ERK phosphorylation and BDNF expression in dopaminergic neurons. These results suggest that, by recruiting cyclic GMP and PKG, neuronal nitric oxide synthase-derived nitric oxide plays a novel and essential role in RAR signaling leading to ERK-dependent BDNF up-regulation in midbrain dopaminergic neurons.
On-line aukce reklamních pozic (2015)

Diplomova prace popisuje vývoj internetu a elektronickeho obchodovani, seznamuje s reklamnimi systemy Seznam Sklik, Google Adwords a Facebook Ads, ktere využiva e-shop lekarna.cz k podpoře prodeje. Jednotlive reklamni kampaně vyhodnocuje a nasledně optimalizuje a zjisťuje, jaký vliv ma optimalizace na výkon kampani. Diplomova prace podava doporuceni, do jakých reklamnich PPC systemu mame investovat penize, pokud chceme zvýsit prodej zboži přes internet v segmentu leků, kosmetiky a zdravotnických pomůcek.
Neural organization of the retina of the turtleMauremys caspica:a light microscope and Golgi study (1988)

The organization of the retina of the turtle species Mauremys caspica, found in fresh water ponds of Israel, has been examined by light microscopical techniques including examination of fresh wholemount retina, one micron blue-stained vertical sections and Golgi-stained material. The anatomical findings on Mauremys retina have been compared with those of the Pseudemys retina (Kolb, 1982) which is more commonly used for electrophysiological and neurochemical studies in the USA. The photoreceptors of Mauremys are similar in type and oil droplet content to Pseudemys photoreceptors except for the double cone in Mauremys. This cone type appears more abundant than in Pseudemys and the principal member contains a yellow oil droplet instead of an orange oil droplet. Golgi staining reveals that the cell types that have been seen in Pseudemys are found in Mauremys with identical morphology. In addition, two amacrine cell types that were not before described for Pseudemys have been added to the classification. One of these is the tristratified dopaminergic amacrine cell described in immunocytochemical studies (Witkovsky et al., 1984; Nguyen-Legros et al., 1985; Kolb et al., 1987). We have used these anatomical studies on Pseudemys and Mauremys retina to form a catalogue of neural types for the turtle retina in general. We conclude with an attempt to combine findings from anatomy, electrophysiology, and neurochemistry to form an overview of the organization of this reptilian retina.
Self-injurious behaviors in children with autism spectrum disorder enrolled in the Study to Explore Early Development (2017)

We assessed potential factors associated with "current" or "ever" self-injurious behaviors, reported in the Autism Diagnostic Interview-Revised, among children with autism spectrum disorder (n = 692) from the Study to Explore Early Development. Data on factors examined were obtained from questionnaires, standardized clinical instruments, and birth certificates. We employed a log-binomial regression to assess these associations. Although most associations were quite similar for currently and ever exhibiting self-injurious behaviors, a few differences were noted. We documented previously unreported associations of current self-injurious behaviors with maternal age and cesarean delivery, and ever self-injurious behaviors with maternal age, child sex, gestational age, and maternal race. We also confirmed previously reported associations with adaptive skills, somatic conditions (sleep, gastrointestinal, and sensory abnormalities), and other behavioral problems. These findings are informative for clinical practice and future research.
Impact of C-reactive Protein (CRP) on Surfactant Function (1989)

Plasma levels of the acute-phase reactant, C-reactive protein (CRP), increase up to one thousand-fold as a result of trauma or inflammation. CRP binds to phosphorylcholine (PC) in a calcium-ion dependent manner. The structural homology between PC and the major phospholipid component of surfactant, dipalmitoyl phosphatidylcholine (DPPC), led to the present study in which we examined if CRP levels might be increased in patients with adult respiratory distress syndrome (ARDS), and subsequently interfere with surfactant function. Our results showed that CRP levels in the bronchoalveolar fluid (BALF) was increased in patients with ARDS (97.8 +/- 84.2 micrograms/mg total protein vs. 4.04 +/- 2.2 micrograms/mg total protein in normals). Our results show that CRP binds to liposomes containing DPPC and phosphatidylglycerol (PG). As a result of this interaction, CRP inhibits the surface activity of a PG-DPPC mixture when tested with a Wilhelmy surfactometer or with the Enhorning pulsating bubble apparatus. Furthermore, the surface activity of a clinically used surfactant replacement, Surfactant TA (2 mg/ml), was also severely impaired by CRP in a dose-dependent manner (doses used ranging from 24.5 to 1,175 micrograms/ml). In contrast, human serum albumin (HSA) at 500 and 900 micrograms/ml had no inhibitory effect on Surfactant TA surface activity. These results suggest that CRP, although not an initiating insult in ARDS, may contribute to the subsequent abnormalities of surfactant function and thus the pathogenesis of the pulmonary dysfunction seen in ARDS.
Enzyme-catalysed hydrolysis of dimethyl 7-hydroxy-3,3-dimethyl-2,4-dioxabicyclo[3.3.0]octane-6,8-dicarboxylate and a novel synthesis of neplanocin A (1992)

Hydrolysis of the diester 1 catalysed by pig liver esterase provided the mono-ester 3. This ester was converted into the amine 9, a precursor of neplanocin 10, thereby confirming the previously proposed configuration of 3.
Lactococcosis in Silver Carp (2014)

An adult Silver Carp Hypophthalmichthys molitrix with a focally extensive skin lesion near the caudal peduncle and mild iridial hemorrhage was submitted to the Aquatic Research and Diagnostic Laboratory (ARDL) in Stoneville, Mississippi, as part of a fish kill investigation. Touch impressions of this musculoskeletal lesion revealed small cocci (∼1 μm) in pairs or chains within an inflammatory milieu. A pure Gram-positive cocci isolate was obtained from the brain, while cultures of the kidney and muscle yielded multiple bacterial colony types, including the Gram-positive cocci seen in the brain. This brain isolate was characterized biochemically and identified as Lactococcus spp. Analysis of the near complete 16S small subunit ribosomal DNA (SSU rDNA) and DNA gyrase subunit B (gyrB) gene sequences revealed the bacterium to be L. lactis subsp. lactis (SSU rDNA: 100% identity, 1,372/1,372 bp; gyrB: 99.7% identity, 1,779/1,785 bp). Comparatively, at the gyrB locus the case isolate shared less than 90% similarity to L. lactis subsp. cremoris (1,599/1,781 bp) and less than 80% homology to L. garvieae (1409/1775 bp). Histopathological examination confirmed a severe meningoencephalitis, a moderate mononuclear myositis, and a mild interstitial nephritis. We believe this represents the first report of a natural infection by L. lactis subsp. lactis in Silver Carp.
Carbon nanotube interconnects: implications for performance, power dissipation and thermal management (2006)

This paper presents a comprehensive evaluation of carbon nanotube bundle interconnects from all aspects critical to VLSI circuits - performance, power dissipation and reliability - while taking into account practical limitations of the technology. A novel delay model for CNT bundle interconnects has been developed, using which it is shown that CNT bundles can significantly improve the performance of long global interconnects with minimal additional power dissipation (for maximum metallic CNT density). While it is well known that CNT bundle interconnects can carry much higher current densities than copper, their impact on back-end thermal management and interconnect temperature rise is presented here for the first time. It is shown that the use of CNT bundle vias integrated with copper interconnects can improve copper interconnect lifetime by two orders of magnitude and also reduce optimal global interconnect delay by as much as 30%
Mission-oriented middleware for sensor-driven scientific systems (2011)

Abstract Coordinating the networks needed for modern scientific data collection and control presents a significant challenge: they are typically power- and resource-constrained, operating in noisy and hostile environments, and need to adapt their behaviour to match their operation to their sensed environment while maintaining the scientific integrity of their observations. In this paper, we explore several aspects of managing and coordinating sensor-driven systems. We draw some conclusions about how to design, structure, and implement programming and middleware abstractions for long-lived and adaptive sensor networks.
Structural function of C‐terminal amidation of endomorphin (2005)

To investigate the structural function of the C-terminal amide group of endomorphin-2 (EM2, H-Tyr-Pro-Phe-Phe-NH(2)), an endogenous micro-opioid receptor ligand, the solution conformations of EM2 and its C-terminal free acid (EM2OH, H-Tyr-Pro-Phe-Phe-OH) in TFE (trifluoroethanol), water (pH 2.7 and 5.2), and aqueous DPC (dodecylphosphocholine) micelles (pH 3.5 and 5.2) were investigated by the combination of 2D (1)H-NMR measurement and molecular modelling calculation. Both peptides were in equilibrium between the cis and trans rotamers around the Tyr--Pro w bond with population ratios of 1 : 1 to 1 : 2 in dimethyl sulfoxide, TFE and water, whereas they predominantly took the trans rotamer in DPC micelle, except in EM2OH at pH 5.2, which had a trans/cis rotamer ratio of 2 : 1. Fifty possible 3D conformers were generated for each peptide, taking different electronic states depending on the type of solvent and pH (neutral and monocationic forms for EM2, and zwitterionic and monocation forms for EM2OH) by the dynamical simulated annealing method, under the proton-proton distance constraints derived from the ROE cross-peak intensities. These conformers were then roughly classified into four groups of two open [reverse S (rS)- and numerical 7 (n7)-type] and two folded (F1- and F2-type) conformers according to the conformational pattern of the backbone structure. Most EM2 conformers in neutral (in TFE) and monocationic (in water and DPC micelles) forms adopted the open structure (mixture of major rS-type and minor n7-type conformers) despite the trans/cis rotamer form. On the other hand, the zwitterionic EM2OH in TFE, water and DPC micelles showed an increased population of F1- and F2-type folded conformers, the population of which varied depending on their electronic state and pH. Most of these folded conformers took an F1-type structure similar to that stabilized by an intramolecular hydrogen bond of (Tyr1)NH(3) (+)...COO(-)(Phe4), observed in its crystal structure. These results show that the substitution of a carboxyl group for the C-terminal amide group makes the peptide structure more flexible and leads to the ensemble of folded and open conformers. The conformational requirement of EM2 for binding to the micro-opioid receptor and the structural function of the C-terminal amide group are discussed on the basis of the present conformational features of EM2 and EM2OH and a possible model for binding to the micro-opioid receptor, constructed from the template structure of rhodopsin.
Phosphoproteomic Analysis of Synaptosomes from Human Cerebral Cortex (2005)

Protein phosphorylation is a crucial post-translational modification mechanism in the regulation of synaptic organization and function. Here, we analyzed synaptosome fractions from human cerebral cortex obtained during therapeutic surgery. To minimize changes in the phosphorylation state of proteins, the tissue was homogenized within two minutes of excision. Synaptosomal proteins were digested with trypsin and phosphopeptides were isolated by immobilized metal affinity chromatography and analyzed by liquid chromatography and tandem mass spectrometry. The method allowed the detection of residues on synaptic proteins that were presumably phosphorylated in the intact cell, including synapsin 1, syntaxin 1, and SNIP, PSD-93, NCAM, GABA-B receptor, chaperone molecules, and protein kinases. Some of the residues identified are the same or homologous to sites that had been previously described to be phosphorylated in mammals whereas others appear to be novel sites which, to our knowledge, have not been reported previously. The study shows that new phosphoproteomic strategies can be used to analyze subcellular fractions from small amounts of tissue for the identification of phosphorylated residues for research and potentially for diagnostic purposes.
Use of Magnet Technology for Phosphate Elimination from Municipal Sewage (1998)

The present report describes the set-up and first operation results of a novel carousel magnetic separator. This carousel magnetic separator is suitable for continuous separation of magnetized particles from water. Continuous operation is achieved by a segmented rotating separation matrix. This matrix also allows the use of low-cost permanent magnets, by means of which magnetic fields of up to 0.5 T can be generated without energy consumption. The suitability of a first prototype of this carousel magnetic separator was tested for elimination of magnetite-containing iron phosphate and iron hydroxide flocs. These flocs result from conventional precipitation/flocculation of municipal sewage with FeCl3 and the addition of magnetite particles generated by wet-chemical processes. First results demonstrate that the carousel magnetic separator can reduce phosphate concentrations of up to 14 mg/L PO43–-P in the inlet to below 1 mg/L PO43–-P in the outlet at filtration rates of about 40 m/h. Magnetite dosage in the range of 50 mg/L affects phosphate precipitation positively via a slight reduction of the precipitant volume. However, the total amount of chemicals required for phosphate elimination is increased by the substances needed for magnetite production. Therefore, a further reduction of magnetite dosage must be achieved. Einsatz von Magnettechnologie im Zuge der Phosphatelimination aus kommunalen Abwässern In der vorliegenden Arbeit werden der Aufbau und erste Betriebsergebnisse eines neuartigen Karussell-Magnetseparators beschrieben. Der Karussell-Magnetseparator eignet sich zur kontinuierlichen Abscheidung magnetisierbarer Partikel aus Wässern. Die kontinuierliche Arbeitsweise wird durch den Einsatz einer segmentierten sowie drehbaren Abscheidematrix erreicht. Diese ermöglicht zusätzlich die Verwendung kostengünstiger Permanentmagnete, die ohne Energieverbrauch in der Lage sind, Flußdichten bis zu 0.5 T zu erzeugen. Ein erster Prototyp des Karussell-Magnetseparators wurde auf seine Eignung zur Elimination magnetithaltiger Eisenphosphat- bzw. Eisenhydroxidflocken hin untersucht. Die Flocken resultierten aus einer konventionellen Fällung/Flockung des häuslichen Abwassers des Forschungszentrums Karlsruhe mit FeCl3 unter Zusatz von naßchemisch erzeugten Magnetitpartikeln. Erste Ergebnisse zeigen, daß der Karussell-Magnetseparator in der Lage ist, ausgehend von einer Phosphatkonzentration im Zulauf von bis zu 14 mg/L PO33–-P, bei Filtergeschwindigkeiten von ca. 40 m/h eine Phosphatkonzentration von 1 mg/L PO43–-P im Ablauf zu unterschreiten. Die Magnetitdosierung im Bereich von 50 mg/L beeinflußt dabei die Phosphatfällung positiv, so daß eine leichte Reduzierung der benötigten Fällmittelmenge erreicht werden kann. Durch die zur Herstellung des Magnetits benötigten Stoffe erhöht sich aber insgesamt die Menge der zur Phosphatelimination benötigten Chemikalien, so daß eine weitere Reduzierung der erforderlichen Magnetitdosierung angestrebt werden muß.
Total Synthesis of Ecumicin (2018)

The first total synthesis of the potent anti-mycobacterial cyclic depsipeptide natural product ecumicin is described. Synthesis was achieved via a solid-phase strategy, incorporating the synthetic non-proteinogenic amino acids N-methyl-4-methoxy-l-tryptophan and threo-β-hydroxy-l-phenylalanine into the growing linear peptide chain. The synthesis employed key on-resin esterification and dimethylation steps as well as a final macrolactamization between the unusual N-methyl-4-methoxy-l-tryptophan unit and a bulky N-methyl-l-valine residue. The synthetic natural product possessed potent antimycobacterial activity against the virulent H37Rv strain of Mycobacterium tuberculosis (MIC<sub>90</sub> = 312 nM).
Strengthening of ferrous binder jet 3D printed components through bronze infiltration (2017)

Net-shape 98% dense objects have been fabricated from a rapidly solidified ferrous powder using binder jet 3D printing and molten bronze infiltration. X-ray diffraction, scanning electron microscopy, and differential thermal analysis were used to characterize the structural evolution of the powder feedstock during an infiltration heating cycle. Microindentation and bend tests were performed on the infiltrated material to evaluate its mechanical properties. It was found that infiltration improved the strength of the sintered preforms by eliminating the stress concentration points at interparticle necks.
Author Correction: High p16 expression and heterozygous RB1 loss are biomarkers for CDK4/6 inhibitor resistance in ER+ breast cancer (2022)

Palafox, Marta; Monserrat, Laia; Bellet Ezquerra, Meritxell; Villacampa Javierre, Guillermo; Gonzalez-Perez, Abel; Oliveira, Mafalda; Brasó-Maristany, Fara; Ibrahimi, Nusaibah; Kannan, Srinivasaraghavan; Mina, Leonardo; Herrera-Abreu, Maria Teresa; Òdena, Andreu; Sánchez-Guixé, Mònica; Capelán, Marta; Azaro, Analía; Bruna, Alejandra; Rodríguez, Olga; Guzmán, Marta; Grueso, Judit; Viaplana, Cristina; Hernandez-Losa, Javier; Su, Faye; Lin, Kui; Clarke, Robert B.; Caldas, Carlos; Arribas, Joaquín V; Michiels, Stefan; García-Sanz, Alicia; Turner, Nicholas C.; Prat, Aleix; Nuciforo, Paolo; Dienstmann, Rodrigo; Verma, Chandra S.; Lopez-Bigas, Nuria; Scaltriti, Maurizio; Arnedos, Monica; Saura Manich, Cristina; Serra, Violeta
Spectro-imagerie endoscopique d'autofluorescence et cartographie 2D pour la localisation et le diagnostic in situ de lésions cancéreuses (2007)

Early diagnosis is the most efficient way to struggle against cancer. Among all the existing techniques, optical methods&#13;\n(photodiagnosis from NUV to NIR) show important characteristics required by the physicians : high sensitivity, non-ionising radiations and non-traumatic measurements. They are particularly well suited to the detection of cancers&#13;\nin hollow organs, that are usually superficial and hardly visible with classical endoscopy. This paper describes a&#13;\nmethodological approach based on the use of tissue autofluorescence, applicable in clinical endoscopy, and leading to&#13;\nthe definition of diagnosis indicators from the spectral parameters. Following a state-of-the-art on autofluorescence&#13;\nspectroscopic (LIFS) and endoscopic imaging methods, we present the efficiency of fibered LIFS in terms of sensitivity&#13;\nand specificity for the diagnosis of esophagus cancerous lesions (clinical study over 25 patients). We then present the&#13;\ntechnological characteristics of an autofluorescence endoscopic imaging prototype developed in our labs as well as its&#13;\ncalibration. A second part is devoted to endoscopic image registration and mosaicing and to optics aberration&#13;\ncorrection in perspective of the automatic construction of a panoramic image (cartography) of the organ’s explored&#13;\nareas. Finally, exploiting the fluorescence data provided by the imager, the feasibility of the superimposition of spatial&#13;\nand spectral information is validated with a phantom.
From “Strong” to “Much Stronger”: Utilization of Green Tea Extract Dispersant for SWCNT‐Reinforced Polymer Composites (2012)

Abstract PVA is reinforced with SWCNTs using green tea extract as a dispersant to achieve good dispersion of the SWCNTs in an organic solvent and finally high‐performance composite fibers. A combination of green tea extract/DMSO/SWCNT/PVA is found to disperse larger aggregates of SWCNTs to individual or thin bundles of a few nanotubes. Incorporation of 0.5 wt% SWCNTs into PVA fibers yields a tensile strength of 2.42 GPa, a Young's modulus of 46 GPa, toughness of 105 J · g −1 at a failure strain of 11%, and a loop strength of 245 MPa, much higher than the values of commercial PVA fibers. The incorporation of highly dispersed SWCNTs suppresses the fibrillation tendency of the PVA fibers. The applied load is effectively transferred from the matrix to the SWCNTs. magnified image