gebhart/patrepeval huggingface dataset:
Air-coupled excitation of rocking motion of individual microspheres on surfaces (2007)

In current study, it is demonstrated that the rocking motion of a single microsphere can be excited by means of impulsive acoustic fields generated by air-coupled transduction and the transient response of the particle can be detected optically. Such observation indicates the existence of rolling moment resistance of particle-surface adhesion bonds. A set of experiments were conducted for measuring the rocking resonance frequency of a microsphere on a flat substrate. The waveforms corresponding to the transient out-of-plane displacement of the rocking particle are used to extract the work of adhesion of the bond between the particle and substrate. Some of the frequency responses of the particles exhibited small shifts in their peak frequencies, which are away from their resonance frequencies, when compared to those of the base motion. These shifts are attributed to the viscoelastic damping effect due to capillary water meniscus formation in the adhesion bond of the particle-substrate system.
The upgrade of SRRC booster extraction system (1999)

It is planned to upgrade the SRRC booster from 1.3 GeV to 1.5 GeV for full energy injection into the storage ring before the end of 1999. Based on the technical specification and operation experience, it is concluded that some of the subsystem components need to be rebuilt, such as the magnet power supply systems and beam extraction elements. A review of the power supply system will be reported elsewhere. In this report, the brief review of the beam extraction process is given according to the 1.5 GeV parameters. The test result of a modified kicker magnet is presented.
Temporal Separation Detection for Chipless Depolarizing Frequency-Coded RFID (2016)

This paper studies the detection of chipless frequency-coded tags. The detection exploits a temporal separation that allows obtaining the identification (ID) with only one measurement. In this way, the flexibility of reading of this type of chipless tags is clearly improved, which is highly expected for future real implementation. This temporal separation is possible when the tag presents a long-time signature, longer than the backscattering wave corresponding to the surrounding objects. A technique based on the short-time Fourier transform (STFT) is used to differentiate the useful parts of the signal, which contain the tag ID. Up to now, this was done by using a calibration process based on two measurements at least to remove coupling and clutter contribution. With the proposed approach the acquisition of the tag ID is direct, and it is not necessary to have further information such as the measurement of the environment without the tag. A study on the time duration of several frequency-coded tags is performed based on simulations and measurements. The study shows that this approach can be used with classical depolarizing chipless tags already proposed in the literature. It is proven that the proposed approach is useful to detect the tag response with a single measurement.
In Situ Hybridization of Adrenergic Receptor mRNA in Brain (2003)

The advent of transgenic mice has ushered in an explosion of new knowledge regarding key roles of adrenergic receptors (ARs) in central nervous system- (CNS) mediated blood pressure control, states of alertness/sedation, analgesic pathways, and temperature control; these findings have renewed interest in examining human CNS tissue for expression of adrenergic receptor subtype mRNA in specific brain regions. In situ hybridization enables detection of rare mRNA species in specific cells within the human CNS. However, identification of adrenergic receptor mRNA in human tissues is difficult owing to restricted access to tissue for harvest immediately after death. Therefore, in situ hybridization methods need to be carefully optimized to preserve remaining mRNA signal in human studies. Although [α-35S]uridine triphosphate (UTP) isotopic in situ hybridization is our preferred method, a summary of nonisotopic in situ hybridization is also included (in Subheading 4.). [α-35S]-UTP isotopic in situ hybridization is highly sensitive, enabling identification of rare RNA species in human tissues (1). We have had best results in human tissues when the actions of tissue RNases on mRNA signal can be arrested within 2 h of death. We have published identification of α2-AR mRNA in human spinal cord using this technique (2). This chapter is designed to facilitate the experienced researcher switching from nonhuman models to human tissues, as well as providing the investigator unfamiliar with in situ hybridization with a step-by-step approach.
System-level performance of downlink NOMA combined with SU-MIMO for future LTE enhancements (2014)

This paper investigates the system-level performance of downlink non-orthogonal multiple access (NOMA) combined with single user MIMO (SU-MIMO) for future LTE (Long-Term Evolution) enhancements. The goal is to clarify the performance gains of NOMA combined with SU-MIMO transmission, taking into account the LTE radio interface such as frequency-domain scheduling, adaptive modulation and coding (AMC), and NOMA specific functionalities such as multi-user pairing/ordering and transmit power allocation. In particular, we propose practical schemes to efficiently combine NOMA with open-loop SU-MIMO (Transmission Mode 3: TM3) and closed-loop SU-MIMO (Transmission Mode 4: TM4) specified in LTE. Based on computer simulations, we compare NOMA performance gains for different granularities of scheduling and MCS (modulation and coding scheme) selection, for both genie-aided channel quality information (CQI) estimation and approximated CQI estimation, and using different number of power sets. Evaluation results show that NOMA can still provide a hefty portion of its expected gains even with approximated CQI estimation and limited number of power sets, and also when LTE compliant subband scheduling and wideband MCS are applied.
Abstract 3138: The lung pro-thrombotic niche drives cancer-associated thromboembolism via exosomal ITGB2 (2022)

Abstract Thromboembolism (TE) is a common complication in cancer patients and the second leading cause of cancer-related deaths. The incidence of TE varies in different cancer types, with the highest risk in lung cancer and pancreatic ductal adenocarcinoma (PDAC), and in advanced-stage and metastatic cancers. Despite the benefits associated with thromboprophylaxis for symptomatic TE, the prevention of TE still remains an unmet clinical need due to lack of biomarkers predictive of TE risk and the bleeding risk associated with the routine use of anti-coagulants. Exosomes are small circulating extracellular vesicles that mediate cell-to-cell communication. Cancer cells and the tumor microenvironment release large numbers of exosomes into the blood circulation and have displayed a therapeutic and predictive value in systemic diseases. Integrins expressed on the surface of exosomes drive their selective organotropism and prepare distant sites for metastatic seeding by establishing favorable pre-metastatic niches. Here we show that exosomes from metastasis-bearing lungs or pre-metastatic lungs of mice with melanoma, breast, lung and pancreatic cancer induce TE in mice and express high levels of integrin beta 2 (ITGB2). Instead, exosomes from tumor cell lines, primary tumors or other metastasis-bearing organs did not show any pro-thrombotic properties. Myeloid cells including monocytes/macrophages and neutrophils infiltrating pre- and post-metastatic lungs were the main source of ITGB2+ pro-thrombotic exosomes. Blockade of ITGB2 on lung-derived exosomes, or systemically in mice, prevented exosome-induced platelet aggregation and TE, and reduced metastasis. Examination of the mechanisms of ITGB2-induced TE showed that exosomal ITGB2 interact directly or through fibrin with different binding partners on platelets, and induce their activation and aggregation. Importantly, we found that exosomal ITGB2 levels are elevated in the plasma of PDAC patients prior to TE events in comparison to PDAC patients with no history of TE, and thus might serve as prognostic biomarker of TE. Together, our results provide the first evidence of the establishment of a pro-thrombotic lung niche in different cancer types. Moreover, we identify exosomal ITGB2 as a new target for the prevention and/or treatment of TE, as well as a potential “liquid biopsy” analyte for the early stratification of patients at high risk of TE. Citation Format: Serena Lucotti, Yusuke Ogitani, Candia M. Kenific, Linda Bojmar, Michele Cioffi, Pernille Lauritzen, Henrik Molina, Soren Heissel, Harry B. Lengel, Xiaohong Jing, Haiying Zhang, Irina Matei, Eileen M. O'Reilly, William R. Jarnagin, David R. Jones, James B. Bussel, David Kelsen, Jacqueline F. Bromberg, Diane M. Simeone, David Lyden. The lung pro-thrombotic niche drives cancer-associated thromboembolism via exosomal ITGB2 [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 3138.
Determining the Volume of Additive Solution and Residual Plasma in Whole Blood Filtered and Buffy Coat Processed Red Cell Concentrates (2015)

Disparity between plasma volume calculation methods was observed. Efforts should be made to standardize residual plasma volume measurement methods in order to accurately assess the impact of residual plasma on transfusion outcomes.
Ultralight High‐Efficiency Flexible InGaP/(In)GaAs Tandem Solar Cells on Plastic (2012)

Ultralight flexible InGaP/(In)GaAs tandem solar cells with conversion efficiency of 28.1% are demonstrated using our novel layer transfer technique, called controlled spalling. Excellent stability of the flexible solar cell characteristics under different bending conditions in conjunction with its remarkably high specific power of 1995 W/kg indicates the prospects of the controlled spalling technology for enabling a viable high-efficiency flexible photovoltaic technology.
Antifungal drug susceptibility testing (1994)

Antifungal drug susceptibility testing Get access J. Van Cutsem, J. Van Cutsem Search for other works by this author on: Oxford Academic PubMed Google Scholar H. Kurata, H. Kurata Search for other works by this author on: Oxford Academic PubMed Google Scholar H. Matsuoka, H. Matsuoka Search for other works by this author on: Oxford Academic PubMed Google Scholar Y. Mikami, Y. Mikami Search for other works by this author on: Oxford Academic PubMed Google Scholar M.A. Pfaller, M.A. Pfaller Search for other works by this author on: Oxford Academic PubMed Google Scholar G.M. Scalarone, G.M. Scalarone Search for other works by this author on: Oxford Academic PubMed Google Scholar M.G. Rinaldi M.G. Rinaldi Search for other works by this author on: Oxford Academic PubMed Google Scholar Journal of Medical and Veterinary Mycology, Volume 32, Issue Supplement_1, December 1994, Pages 267–276, https://doi.org/10.1080/02681219480000891 Published: 01 December 1994
Systematic Identification of Culture Conditions for Induction and Maintenance of Naive Human Pluripotency (2014)

(Cell Stem Cell 15, 471–487; October 2, 2014) Because of a production issue with this paper, the initial version posted on July 24, 2014 was not the final approved version and contained textual errors. It was replaced by the correct version of the article on July 25, 2014. In response to a reader query, the authors would also like to clarify that, although the graphical abstract indicates that reduction of bivalent domains was induced in 5i conditions, the actual experiment involved for this analysis also included a JNK inhibitor and thus used 6i conditions (Figure 7) as indicated in the manuscript text. Systematic Identification of Culture Conditions for Induction and Maintenance of Naive Human PluripotencyTheunissen et al.Cell Stem CellJuly 24, 2014In BriefThrough sequential chemical screening, Theunissen et al. identify a combination of kinase inhibitors that induces and maintains defining features of naive pluripotency in human embryonic stem cells. Full-Text PDF Open Access
Substituted 3-Imidazo[1,2-a]pyridin-3-yl- 4-(1,2,3,4-tetrahydro-[1,4]diazepino- [6,7,1-hi]indol-7-yl)pyrrole-2,5-diones as Highly Selective and Potent Inhibitors of Glycogen Synthase Kinase-3 (2004)

Glycogen synthase kinase-3 (GSK3) is involved in signaling from the insulin receptor. Inhibitors of GSK3 are expected to effect lowering of plasma glucose similar to insulin, making GSK3 an attractive target for the treatment of type 2 diabetes. Herein we report the discovery of a series of potent and selective GSK3 inhibitors. Compounds 7-12 show oral activity in an in vivo model of type II diabetes, and 9 and 12 have desirable PK properties.
20 μs Photocurrent Response from Lithographically Patterned Nanocrystalline Cadmium Selenide Nanowires (2010)

Lithographically patterned nanowire electrodeposition (LPNE) provides a method for patterning nanowires composed of nanocrystalline cadmium selenide (nc-CdSe) over wafer-scale areas. We assess the properties of (nc-CdSe) nanowires for detecting light as photoconductors. Structural characterization of these nanowires by X-ray diffraction and transmission electron microscopy reveals they are composed of stoichiometric, single phase, cubic CdSe with a mean grain diameter of 10 nm. For nc-CdSe nanowires with lengths of many millimeters, the width and height dimensions could be varied over the range from 60 to 350 nm (w) and 20 to 80 nm (h). Optical absorption and photoluminescence spectra for nc-CdSe nanowires were both dominated by band-edge transitions. The photoconductivity properties of nc-CdSe nanowire arrays containing approximately 350 nanowires were evaluated by electrically isolating 5 microm nanowire lengths using evaporated gold electrodes. Photocurrents, i(photo), of 10-100 x (i(dark)) were observed with a spectral response characterized by an onset at 1.75 eV. i(photo) response and recovery times were virtually identical and in the range from 20 to 40 micros for 60 x 200 nm nanowires.
Presynaptic {alpha}2-GABAA receptors in primary afferent depolarization and spinal pain control (2011)

Spinal dorsal horn GABA(A) receptors are found both postsynaptically on central neurons and presynaptically on axons and/or terminals of primary sensory neurons, where they mediate primary afferent depolarization (PAD) and presynaptic inhibition. Both phenomena have been studied extensively on a cellular level, but their role in sensory processing in vivo has remained elusive, due to inherent difficulties to selectively interfere with presynaptic receptors. Here, we address the contribution of a major subpopulation of GABA(A) receptors (those containing the α2 subunit) to spinal pain control in mice lacking α2-GABA(A) receptors specifically in primary nociceptors (sns-α2(-/-) mice). sns-α2(-/-) mice exhibited GABA(A) receptor currents and dorsal root potentials of normal amplitude in vitro, and normal response thresholds to thermal and mechanical stimulation in vivo, and developed normal inflammatory and neuropathic pain sensitization. However, the positive allosteric GABA(A) receptor modulator diazepam (DZP) had almost completely lost its potentiating effect on PAD and presynaptic inhibition in vitro and a major part of its spinal antihyperalgesic action against inflammatory hyperalgesia in vivo. Our results thus show that part of the antihyperalgesic action of spinally applied DZP occurs through facilitated activation of GABA(A) receptors residing on primary nociceptors.
Real-time component integration based on transparent distribution (2005)

This paper introduces a real-time component model that offers a separation of concerns which allows a straight-forward integration of independently developed components. So-called transparent distribution forms the backbone of the integration process. Transparent distribution means that (1) the functional and temporal behavior of a system is the same no matter on which node of a distributed system a component is executed and (2) the developer does not have to care about the differences of local versus distributed execution of a component. We first present the concepts of a component model for real time systems that is well suited for transparent distribution. The component model is based on logical execution time, which abstracts from physical execution time and thereby from both the execution platform and the communication topology. Then we discuss the resulting tool chain and integration process. A case study rounds out the paper.
Extreme Elongation of Vesicles Under DC Electric Fields (2008)

Electrodeformation refers to the deformation of cell or vesicle lipid membranes under the application of an electric field. Such a phenomenon often accompanies electroporation processes, and also can be leveraged to detect pathological changes in cells. Recent studies have suggested that the electrical conductivity difference across the lipid membrane is a dominant factor in determining the characteristics of deformation, and various regimes of deformation were observed. Using a vesicle model system, the current work is the first report of extreme elongation of vesicles of high conductivity ratio under DC electric fields. The results suggest that the osmolarity difference between the encapsulated and bathing solutions may contribute to such abnormal deformation behavior.
Photometric Study of Uranian Satellites (1998)

The best summary of my work at NASA is expressed in the following abstract, submitted the Division for Planetary Science of the American Astronomical Society and to be presented at the annual meeting in Madison in October. We report photometric measurements of Uranian satellites Miranda, Ariel, Umbriel and Titania (10.4 Aug. 1995), and Neptune's satellite Triton (21.2 Sept. 1995) with the infrared camera (IRCAM) and standard J (1.13 - 1.42 microns), H (1.53 - 1.81 microns), and K (2.00 - 2.41 microns) filters at the 3.8-m UKIRT telescope on Mauna Kea. The individual images frames are 256 x 256 pixels with a platescale of .286 arcsec/pixel, resulting in a 1.22 arc min field of view. This summer brought the IR photometry measurements nearly to a close. As indicated by the abstract above, I will present this work at the annual DPS meeting in October. In anticipation of the opening of the new Carl Sagan Laboratory for Cosmochemisty, of which I will be a participating member, I also devoted a considerable fraction of the summer to learning the biochemistry which underlies the experiments to be conducted. To put the end of the summary close to the beginning, it was a most productive summer.
Low temperature production of levulinic acid from cellulose using concentrated phosphoric and sulfuric acid solutions. (2013)

International audience
ESTAÇÃO DE TRABALHO MULTIFUNCIONAL PROJETADA A PARTIR DA ANÁLISE ERGONÔMICA (2015)

A pesquisa desenvolvida representa a importância da interdisciplinaridade para o desenvolvimento de projetos de Tecnologia Assistiva. Tem como principal objetivo o desenvolvimento de uma estação de trabalho multifuncional como recurso de Tecnologia Assistiva, promovendo uma maior autonomia e independência funcional, traduzindo assim uma melhoria na qualidade de vida das pessoas com limitações funcionais. A metodologia utilizada foi da Análise Ergonômica, onde a demanda, tarefa e atividade se caracterizaram como suporte das fases de pesquisa. Como resultado das análises e avaliações, foram geradas modelagens tridimensionais e o desenvolvimento de um protótipo funcional, que cumpriu plenamente com os requisitos de projeto, resultando em um produto de qualidade técnica, funcional e estética.
Enzymatic synthesis of oligo-d-galactofuranosides and l-arabinofuranosides: from molecular dynamics to immunological assays (2010)

D-Galactofuranosyl-containing conjugates are ubiquitous in many pathogenic microorganisms, but completely absent from mammals. As they may constitute interesting pharmacophores, recent works have been dedicated to their preparation. Besides well-reported chemical procedures, enzymatic approaches are still limited, mainly due to the lack of the corresponding biocatalysts. Based on the similarity between chemical structures, the arabinofuranosyl hydrolase Araf51 from Clostridium thermocellum was expected to recognize both the L-Araf motif and its D-Galf analogue. Molecular dynamics and STD-NMR were firstly used to confirm this hypothesis and increase our knowledge of the active site. Interestingly, this arabinofuranosidase was not only able to hydrolyze galactosyl derivatives, but was also really efficient in catalyzing oligomerisations using p-nitrophenyl furanosides as donors. The structures of the products obtained were determined using mass spectrometry and NMR. Amongst them, all the possible regioisomers of di-arabino and -galactofuranosides were synthesized, and the ratio of each regioisomer was easily tuned with respect to the reaction time. Especially, the galactofuranobioside displaying the biologically relevant sequence beta-D-Galf-(1,6)-beta-D-Galf was enzymatically prepared for the first time. All fractions going from di- to penta-arabino- and galactofuranosides were tested for their ability in eliciting the production of TNF-alpha. Interesting immunological properties were observed with arabinofuranosides as short as three sugar residues.
Constitutive expression of Botrytis aclada laccase in Pichia pastoris (2012)

The heterologous expression of laccases is important for their large-scale production and genetic engineering--a prerequisite for industrial application. Pichia pastoris is the preferred expression host for fungal laccases. The recently cloned laccase from the ascomycete Botrytis aclada (BaLac) has been efficiently expressed in P. pastoris under the control of the inducible alcohol oxidase (AOX1) promoter. In this study, we compare these results to the constitutive expression in the same organism using the glyceraldehyde-3-phosphate dehydrogenase (GAP) promoter. The results show that the amounts of BaLac produced with the GAP system (517 mgL(-1)) and the AOX1 system (495 mgL(-1)) are comparable. The constitutive expression is, however, faster, and the specific activity of BaLac in the culture supernatant is higher (41.3 Umg(-1) GAP, 14.2 Umg(-1) AOX1). In microtiter plates, the constitutive expression provides a clear advantage due to easy manipulation (simple medium, no methanol feeding) and fast enzyme production (high-throughput screening assays can already be performed after 48 h).