MP52-15 KIDNEY REGENERATION WITH BIOMIMETIC VASCULAR SCAFFOLDS BASED ON VASCULAR CORROSION CASTS (2020)

You have accessJournal of UrologyTransplantation & Vascular Surgery: Renal Transplantation & Vascular Surgery II (MP52)1 Apr 2020MP52-15 KIDNEY REGENERATION WITH BIOMIMETIC VASCULAR SCAFFOLDS BASED ON VASCULAR CORROSION CASTS Jennifer Huling, Sang-Il Min, Doo Sang Kim, John Jackson*, In Kap Ko, Ji Hyun Kim, Anthony Atala, and James Yoo Jennifer HulingJennifer Huling More articles by this author , Sang-Il MinSang-Il Min More articles by this author , Doo Sang KimDoo Sang Kim More articles by this author , John Jackson*John Jackson* More articles by this author , In Kap KoIn Kap Ko More articles by this author , Ji Hyun KimJi Hyun Kim More articles by this author , Anthony AtalaAnthony Atala More articles by this author , and James YooJames Yoo More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000000914.15AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Vascularization is one of the major hurdles affecting the survival and integration of implanted three-dimensional tissue constructs in vivo. We have developed a biomimetic renal vascular scaffold based on a vascular corrosion casting technique. This study evaluated the feasibility of using this novel biomimetic scaffold for kidney regeneration in a rat kidney cortical defect model. METHODS: Vascular corrosion casts were prepared from normal rat kidneys by perfusion with 10% polycaprolactone (PCL) solution, followed by tissue digestion. The corrosion PCL cast was coated with collagen, and PCL was removed from within the collagen coating, leaving only a hollow collagen-based biomimetic vascular scaffold. The fabricated scaffolds were pre-vascularized with MS1 endothelial cell coating, incorporated into 3D renal constructs, and subsequently implanted either with or without human renal cells in the renal cortex of nude rats. RESULTS: The implanted collagen-based vascular scaffold was easily identified and integrated into native kidney tissue. The biomimetic vascular scaffold coated with endothelial cells (MS1) showed significantly enhanced vascularization, as compared to the uncoated scaffold and hydrogel only groups (P<0.001). Along with the improved vascularization effects, the MS1-coated scaffolds showed a significant renal cell infiltration from the neighboring host tissue, as compared to the other groups (P<0.05). Moreover, addition of human renal cells to the MS1-coated scaffold resulted in further enhancement of vascularization and tubular structure regeneration within the implanted constructs. The biomimetic collagen vascular scaffolds coated with endothelial cells are able to enhance vascularization and facilitate the formation of renal tubules after 14 days when combined with human renal cells. CONCLUSIONS: This study shows the feasibility of bioengineering vascularized functional renal tissues for kidney regeneration. The use of this scaffold system could address the challenges associated with vascularization, which may be an ideal treatment strategy for partial augmentation in patients with chronic kidney disease. Source of Funding: Department funding © 2020 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 203Issue Supplement 4April 2020Page: e778-e778 Advertisement Copyright & Permissions© 2020 by American Urological Association Education and Research, Inc.MetricsAuthor Information Jennifer Huling More articles by this author Sang-Il Min More articles by this author Doo Sang Kim More articles by this author John Jackson* More articles by this author In Kap Ko More articles by this author Ji Hyun Kim More articles by this author Anthony Atala More articles by this author James Yoo More articles by this author Expand All Advertisement PDF downloadLoading ...