gebhart/patrepeval huggingface dataset:
Ocular tolerability and efficacy of intravitreal and subconjunctival injections of sirolimus in patients with non-infectious uveitis: primary 6-month results of the SAVE Study (2013)

Local administration of sirolimus, either intravitreally or subconjunctivally, appears to be safe and tolerable. No drug-related systemic adverse events or serious adverse events were noted. Sirolimus delivered as either an intravitreal or subconjunctival injection has demonstrated bioactivity as an immunomodulatory and corticosteroid-sparing agent in reducing vitreous haze and cells, improving visual acuity, and in decreasing the need for systemic corticosteroids.
97 SUPPLEMENTATION WITH FOLATE IN VITRO INCREASES TROPHECTODERM AND TOTAL CELL NUMBER IN IN VITRO-DERIVED PORCINE BLASTOCYSTS (2011)

It is vital that improvements are made to current culture environments because in vitro culture systems are suboptimal compared with in vivo. A previous transcriptional profiling endeavour conducted by Bauer et al. (2010 Biol. Reprod.83, 791–798) identified hundreds of mRNA transcripts that were mis-expressed in porcine embryos fertilized in vivo and then cultured in vitro to Day 6 compared with in vivo Day-6 embryos. Enriched in the downregulated transcripts were 4 genes involved with the one carbon pool by folate KEGG pathway. This downregulation of genes involved with folate metabolism may illustrate an impaired folate homeostasis in embryos cultured in the current culture environment. The objective of this study was to determine the effects folate had on embryo development of in vitro fertilized embryos. Porcine cumulus–oocyte complexes were matured for 44 h in M199 supplemented with epidermal growth factor (EGF), FSH and LH. Oocytes with a visible polar body were selected and fertilized in modified tris buffered medium for 5 h and then placed into porcine zygote medium 3 with 0 mM, 0.2 mM, 0.4 mM and 0.8 mM folate to find the optimal concentration of folate. Twenty-eight hours post-fertilization, cleaved embryos were selected and moved into 25-µL drops of respective culture medium and cultured to Day 6 in a water-saturated atmosphere of 5% CO2, 5% O2, 90% N2, at 38.5°C. To determine the effect folate had on development, the blastocyst rate for each treatment group was measured. Results were log-transformed and analysed by using PROC GLM in SAS (SAS Institute Inc., Cary, NC). A least-significant difference post-test comparison was completed to determine if significant differences existed between treatment groups. The percentage of cleaved embryos on Day 6 that developed to blastocyst was 56.2%, 55.9%, 66.9% and 61.8% (n = 133, 149, 135 and 135) in 0 mM, 0.2 mM folate, 0.4 mM folate and 0.8 mM, respectively. The 0.4 mM folate group tended (P = 0.07) to have a higher number of cleaved embryos that developed to the blastocyst stage. Consequently, this concentration was used for all further embryo culture experiments. Differential staining was completed to compare the number of trophectoderm and inner cell mass nuclei for embryos cultured in 0 mM or 0.4 mM folate concentrations. Staining revealed that embryos cultured with folate had an increase in number of trophectoderm (29.7 ± 1.5 vs 24.4 ± 1.4 cells; P = 0.0058) and total cell (36.9 ± 1.0 vs 31.7 ± 1.0; P = 0.0007) numbers compared with embryos cultured without folate. These results illustrate that the addition of folate to current culture medium doesn't hinder development to blastocyst and by increasing trophectoderm and total cell number may give rise to better-quality in vitro-derived embryos. It is evident that using transcriptional profiling can be a great method of identifying ways to improve embryo culture systems and, in this case, supplementing with folate. Funded by Food for the 21st Century.
Data from Killing of Resistant Cancer Cells with Low Bak by a Combination of an Antimesothelin Immunotoxin and a TRAIL Receptor 2 Agonist Antibody (2023)

&lt;div&gt;Abstract&lt;p&gt;&lt;b&gt;Purpose:&lt;/b&gt; Many solid tumors express cell surface mesothelin making them attractive targets for antibody-based therapies of cancer. SS1P [antimesothelin(Fv)PE38] is a recombinant immunotoxin (RIT) that has potent cytotoxic activity on several cancer cell lines and clinical activity in mesothelioma patients. Pancreatic cancers express mesothelin and are known to be resistant to most chemotherapeutic agents. The goal of this study is to treat pancreatic cancer with RIT by targeting mesothelin.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Experimental Design:&lt;/b&gt; We measured the cytotoxic activity of an antimesothelin immunotoxin on pancreatic cancer cells. We also measured the levels of several pro- and antiapoptotic proteins, as well as the ability of TNF-related apoptosis-inducing ligand (TRAIL) or the anti-TRAIL receptor 2 agonist antibody (HGS-ETR2) to kill pancreatic cells, and the cytotoxic activity of the two agents together in cell culture and against tumors in mice.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Results:&lt;/b&gt; In two pancreatic cancer cell lines, immunotoxin treatment inhibited protein synthesis but did not produce significant cell death. The resistant lines had low levels of the proapoptotic protein Bak. Increasing Bak expression enhanced the sensitivity to immunotoxins, whereas Bak knockdown diminished it. We also found that combining immunotoxin with TRAIL or HGS-ETR2 caused synergistic cell death, and together triggered caspase-8 recruitment and activation, Bid cleavage and Bax activation. Combining SS1P with HGS-ETR2 also acted synergistically to decrease tumor burden in a mouse model.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Conclusion:&lt;/b&gt; Our data show that low Bak can cause cancer cells to be resistant to immunotoxin treatment and that combining immunotoxin with TRAIL or a TRAIL agonist antibody can overcome resistance. &lt;i&gt;Clin Cancer Res; 17(18); 5926–34. ©2011 AACR&lt;/i&gt;.&lt;/p&gt;&lt;/div&gt;
Immunosuppressive drug FTY720 sensitizes prostate cancer cells to radiotherapy by inhibition of sphingosine kinase‐1. (2008)

A sphingolipid analogue FTY720 is a novel immunosuppressive drug – an agonist of sphingosine 1‐phosphate (S1P) receptors. Recently we identified S1P as a therapy target in prostate cancer. In the current study we have demonstrated that FTY720 can radiosensitize prostate cancer in cell and animal models. FTY720 has induced apoptosis in several hormone‐ and radio‐resistant prostate cancer cell lines. FTY720 treatment induced a sustained inhibition of prosurvival sphingosine kinase‐1 (SK1) and a decrease in the intracellular S1P content. Enforced expression of SK1 in prostate cancer cells rendered them resistant to FTY720. In vitro sub‐lethal concentrations of FTY720 have dramatically sensitized PC‐3 cells to radiotherapy in p53‐independent manner. Mice orthotopically engrafted with fluorescent PC‐3 cells were treated with FTY720 with or without ã‐Irradiation. FTY720 has demonstrated a synergy with ã‐irradiation significantly reducing tumor size, angiogenesis and formation of micrometastases as demonstrated by fluorescent in vivo imaging. In conclusion, FTY720 can sensitize prostate cancer cells to radiotherapy both in vitro and in vivo, through inhibition of SK1 and modification of intracellular levels of lipid second messengers.
Syntheses and characterization of the gallium-phosphorus cages {(Et2O)2Li}{t-BuGa}{P(t-Bu)}2{Ga(t-Bu)2} and {(t-Bu)2Ga}{t-BuGa}{P(t-Bu)}2{Ga(t-Bu)2} (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSyntheses and characterization of the gallium-phosphorus cages {(Et2O)2Li}{t-BuGa}{P(t-Bu)}2{Ga(t-Bu)2} and {(t-Bu)2Ga}{t-BuGa}{P(t-Bu)}2{Ga(t-Bu)2}Mark A. Petrie and Philip P. PowerCite this: Organometallics 1993, 12, 5, 1592–1596Publication Date (Print):May 1, 1993Publication History Published online1 May 2002Published inissue 1 May 1993https://pubs.acs.org/doi/10.1021/om00029a017https://doi.org/10.1021/om00029a017research-articleACS PublicationsRequest reuse permissionsArticle Views68Altmetric-Citations16LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Power Converter Sizing for a Switched Doubly Fed Machine Propulsion Drive (2014)

Doubly fed machines (DFMs) can be used to make variable speed drives (VSDs) that not only provide full shaft speed control but also avoid the need for a dc electrical bus capable of providing full mechanical shaft power. This makes the DFM attractive for any VSD application where the primary power source is ac. Switching configuration of the DFM on-the-fly opens up opportunity to operate the DFM on a wider speed range with reduced power electronics. This paper explores the design requirements for a switched DFM rotor power electronics in full-speed-range VSD applications, including propulsion drives. Tradeoffs are examined for power electronics sizing versus transient settling characteristics.
Extension of microglial activation is associated with epilepsy and cognitive dysfunction in Tuberous sclerosis complex: A TSPO-PET study (2022)

Neuroinflammation contributes to the severity of various neurological disorders, including epilepsy. Tuberous sclerosis complex (TSC) is a condition that results in the overactivation of the mammalian target of rapamycin (mTOR) pathway, which has been linked to the activation of microglia responsible for neuroinflammation. To clarify the involvement of neuroinflammation in the neuropathophysiology of TSC, we performed a positron emission tomography (PET) study using the translocator protein (TSPO) radioligand, [11C] DPA713, and investigated microglial activation in relation to neurological manifestations, especially epilepsy and cognitive function. This cross-sectional study included 18 patients with TSC (6 in the no-seizure group, 6 in the refractory seizure group, and 6 in the mTOR-inhibitor [mTOR-i] group). All participants underwent [11C] DPA713-PET. PET results were superimposed with a 3D T2-weighted fluid-attenuated inversion-recovery (FLAIR) and T1-weighted image (T1WI) to evaluate the location of cortical tubers. Microglial activation was assessed using the standardized uptake value ratio (SUVr) of DPA713 binding. The volume ratio of the DPA713-positive area to the intracranial volume (volume ratio of DPA713/ICV) was calculated to evaluate the extent of microglial activation. A correlation analysis was performed to examine the relationship between volume ratio of DPA713/ICV and severity of epilepsy and cognitive function. Most cortical tubers with hyperintensity on FLAIR and hypo- or isointensity on T1WI showed microglial activation. The extent of microglial activation was significantly greater in the refractory seizure group than in the no-seizure or mTOR-i groups (p < 0.001). The extent of microglial activation in subjects without mTOR-i treatment correlated positively with epilepsy severity (r = 0.822, P = 0.001) and negatively with cognitive function (r = -0.846, p = 0.001), but these correlations were not present in the mTOR-i group (r = 0.232, P = 0.658, r = 0.371, P = 0.469, respectively). Neuroinflammation is associated with the severity of epilepsy and cognitive dysfunction in brains with TSC. mTOR-i may suppress the extent of neuroinflammation in TSC. Investigating the spread of microglial activation using TSPO-PET in these patients may help to predict the progression of neuropathy by assessing the degree of neuroinflammation and therefore be useful for determining how aggressive the treatment should be and in assessing the effectiveness of such treatment in patients with TSC.
A TEMPORAL MULTI-FREQUENCY ENCODING TECHNIQUE FOR CHIPLESS RFID BASED ON C-SECTIONS (2013)

A time domain chipless RFID tag based on cascaded microstrip coupled transmission line sections (C-sections), which can operate in multi-frequency bands is presented. The group delay characteristics of the C-sections are exploited to generate the tag Identiflcation (ID). The tag comprises cascaded commensurate group of C-sections and two cross-polarized ultra wide-band (UWB) antennas. Since the proposed tag can operate in multi-frequency bands, this paper proves the possibility of increasing the coding capacity compared to the existing time domain designs. A tag operating at ISM (Industrial Scientiflc and Medical) bands at 2.45GHz and 5.8GHz together with conformance of frequency and power regulations is discussed elaborately. The prototype of the device is fabricated and validated experimentally. The time domain characteristic of the tag is also validated experimentally by interrogating a short pulse. Furthermore, measurement results using commercial Ultra Wide Band (UWB) radar which can be used as a chipless RFID reader is also incorporated. The obtained results conflrm the concept and the possibility of using temporal multi-frequency in chipless RFID.
On Uncertainty Quantification in Materials Modeling and Discovery: Applications of GE's BHM and IDACE (2023)

View Video Presentation: https://doi.org/10.2514/6.2023-0528.vid The coupling of artificial intelligence and materials characterizations has been a center piece of almost all materials discovery efforts since 1990. Furthermore, with the constant development in probabilistic machine learning tools (i.e. machine learning models that can efficiently propagate uncertainty from inputs to outputs), the synergistic interplay between materials expert knowledge and machine learning model accuracy has been a major driver for state-of-the-art materials research. However, there still exist major challenges facing the machine learning community today when it comes to materials discovery. Be it properly representing uncertainties in the data, or leveraging materials expert knowledge in the learning phase, or actively learning from the usually-sparse, and in many cases non-comprehensive, data sets available to the machine learning expert. These challenges need to be overcome to accelerate research endeavors in the domain of material discovery. To this end, General Electric Research (GER) has been developing and applying highly robust, industry-grade tools that can do tasks like uncertainty quantification (UQ), probabilistic calibration, meta-modelling, active learning, and many more. Application and demonstration of two such tools namely Bayesian Hybrid Modeling (GEBHM) and Intelligent Design and Analysis of Computer Experiments (IDACE) are presented in this paper in the context of materials modeling and discovery.
Total Synthesis of (-)-Austalide B. A Generic Solution to Elaboration of the Pyran/p-Cresol/Butenolide Triad (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTotal Synthesis of (-)-Austalide B. A Generic Solution to Elaboration of the Pyran/p-Cresol/Butenolide TriadLeo A. Paquette, Ting-Zhong Wang, and Matthew R. SivikCite this: J. Am. Chem. Soc. 1994, 116, 25, 11323–11334Publication Date (Print):December 1, 1994Publication History Published online1 May 2002Published inissue 1 December 1994https://pubs.acs.org/doi/10.1021/ja00104a012https://doi.org/10.1021/ja00104a012research-articleACS PublicationsRequest reuse permissionsArticle Views1277Altmetric-Citations36LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
Identification of peptides interfering the lrrk2/pp1 interaction (2019)

ABSTRACT Serine/threonine phosphatases are responsible for counteracting the effect of the protein kinases implicated in the development of several pathologies. Here we identified by PEP-scan approach the sequence of a fragment of LRRK2, a Parkinson’s disease associated protein, interacting with the phosphatase PP1. The fragment, that is located in a LRRK2 domain of undefined function, was associated in N-terminal to an optimized cell penetrating peptide in order to study their in vitro and in vivo biological activity. From this original sequence, we developed and studied five interfering peptides (IPs) and identified two peptides able to disrupt the LRRK2/PP1 interaction by in vitro competition in anti-LRRK2 immunoprecipitates. Using FITC-labelled peptides, we confirmed the internalization of the peptides in cell lines as well as in and primary human normal and pathological cells. Finally, we have confirmed by ELISA test the association of Mut3DPT-LRRK2-Long and Mut3DPT-LRRK2-Short peptides to purified PP1 protein in a selective manner. The shortest peptides, MuteDPT-LRRK2-5 to 8 with either N or C-terminal deletions are not able neither disrupt the association LRRK2/PP1 nor to associate to purified PP1 protein. The peptides Mut3DPT-LRRK2-Long and Mut3DPT-LRRK2-Short may be new tools to study the role of LRRK2/PP1 interaction in normal and pathological conditions.
45.4: The White Light‐Emitting Diode Using Organic Material and Perovskite Quantum Dots (2021)

White LED is widely used for large‐area displays and lighting. Here we fabricated the white LED using red and green perovskite quantum dots and blue organic materials. The maximum efficiency of this hybrid white LED device was 2.1 cd/A and has a color coordinate of (0.32, 0.27) which is very close to the required white coordinate of (0.33, 0.33) based on the CIE 1931 color coordinate.
Rotational state distributions of NO molecules after interaction with germanium surfaces (1985)

The rotational state distributions of NO molecules scattered at clean and oxidized Ge surfaces were determined by means of laser induced fluorescence (LIF). For low incident kinetic energies (Ekin≲200 meV) scattering is dominated by trapping/desorption and the molecules coming off the surface are translationally accommodated to the surface temperature. The rotational state populations can in this case be approximated by a Boltzmann distribution. The rotational temperature Trot equals the surface temperature Ts, however only up to about 250 K. Further increase of Ts leads to a leveling off of Trot until a limiting value Trot,lim≈400 K is reached. (Rotational cooling in desorption.) At higher kinetic energies direct-inelastic scattering dominates, and for Ekin=820 meV a pronounced non-Boltzmann distribution (rotational rainbow) is observed. The observed effects are qualitatively interpreted in terms of current theoretical models.
Dopant site-dependent luminescence from rare-earth doped dibarium octafluorohafnate Ba2HfF8 nanocubes for radiation detection (2021)

Rare-earth doped Ba<sub>2</sub>HfF<sub>8</sub> nanocubes as a new scintillator host material with crystallographic site-dependent luminescence for radiation detection.
Molecular Evidence for Nosocomial Transmission of Human Immunodeficiency Virus from a Surgeon to One of His Patients (1998)

We have investigated the molecular evidence in favor of the transmission of human immunodeficiency virus (HIV) from an HIV-infected surgeon to one of his patients. After PCR amplification, the env and gag sequences from the viral genome were cloned and sequenced. Phylogenetic analysis revealed that the viral sequences derived from the surgeon and his patient are closely related, which strongly suggests that nosocomial transmission occurred. In addition, these viral sequences belong to group M of HIV type 1 but are divergent from the reference sequences of the known subtypes.
Asymmetric Modification of Hepatitis B Virus (HBV) Genomes by an Endogenous Cytidine Deaminase inside HBV Cores Informs a Model of Reverse Transcription (2018)

Cytidine deaminases inhibit replication of a broad range of DNA viruses by deaminating cytidines on single-stranded DNA (ssDNA) to generate uracil. While several lines of evidence have revealed hepatitis B virus (HBV) genome editing by deamination, it is still unclear which nucleic acid intermediate of HBV is modified. Hepatitis B virus has a relaxed circular double-stranded DNA (rcDNA) genome that is reverse transcribed within virus cores from a RNA template. The HBV genome also persists as covalently closed circular DNA (cccDNA) in the nucleus of an infected cell. In the present study, we found that in HBV-producing HepAD38 and HepG2.2.15 cell lines, endogenous cytidine deaminases edited 10 to 25% of HBV rcDNA genomes, asymmetrically with almost all mutations on the 5' half of the minus strand. This region corresponds to the last half of the minus strand to be protected by plus-strand synthesis. Within this half of the genome, the number of mutations peaks in the middle. Overexpressed APOBEC3A and APOBEC3G could be packaged in HBV capsids but did not change the amount or distribution of mutations. We found no deamination on pregenomic RNA (pgRNA), indicating that an intact genome is encapsidated and deaminated during or after reverse transcription. The deamination pattern suggests a model of rcDNA synthesis in which pgRNA and then newly synthesized minus-sense single-stranded DNA are protected from deaminase by interaction with the virus capsid; during plus-strand synthesis, when enough dsDNA has been synthesized to displace the remaining minus strand from the capsid surface, the single-stranded DNA becomes deaminase sensitive.<b>IMPORTANCE</b> Host-induced mutation of the HBV genome by APOBEC proteins may be a path to clearing the virus. We examined cytidine-to-thymidine mutations in the genomes of HBV particles grown in the presence or absence of overexpressed APOBEC proteins. We found that genomes were subjected to deamination activity during reverse transcription, which takes place within the virus capsid. These observations provide a direct insight into the mechanics of reverse transcription, suggesting that newly synthesized dsDNA displaces ssDNA from the capsid walls, making the ssDNA accessible to deaminase activity.