gebhart/patrepeval huggingface dataset:
An engineered bispecific DNA-encoded IgG antibody protects against Pseudomonas aeruginosa in a pneumonia challenge model (2017)

The impact of broad-spectrum antibiotics on antimicrobial resistance and disruption of the beneficial microbiome compels the urgent investigation of bacteria-specific approaches such as antibody-based strategies. Among these, DNA-delivered monoclonal antibodies (DMAbs), produced by muscle cells in vivo, potentially allow the prevention or treatment of bacterial infections circumventing some of the hurdles of protein IgG delivery. Here, we optimize DNA-delivered monoclonal antibodies consisting of two potent human IgG clones, including a non-natural bispecific IgG1 candidate, targeting Pseudomonas aeruginosa. The DNA-delivered monoclonal antibodies exhibit indistinguishable potency compared to bioprocessed IgG and protect against lethal pneumonia in mice. The DNA-delivered monoclonal antibodies decrease bacterial colonization of organs and exhibit enhanced adjunctive activity in combination with antibiotics. These studies support DNA-delivered monoclonal antibodies delivery as a potential strategy to augment the host immune response to prevent serious bacterial infections, and represent a significant advancement toward broader practical delivery of monoclonal antibody immunotherapeutics for additional infectious pathogens.DNA-delivered monoclonal antibodies (DMAbs) can be produced by muscle cells in vivo, potentially allowing prevention or treatment of infectious diseases. Here, the authors show that two DMAbs targeting Pseudomonas aeruginosa proteins confer protection against lethal pneumonia in mice.
Preparation and characterization of aluminum borate whisker reinforced magnesium alloy composites by semi-solid process (1999)

To develop a new kind of composite material, 'graded morphology MMCs', a semi-solid magnesium alloy was squeeze-cast into an aluminum borate whisker preform. Currently, to increase the interfacial tensile/shear strength between MMCs and some metal parts, the joining processes have been studied. However, a suitable process has not yet been found. In this study, we first studied the optimum manufacturing condition to increase the preform compressive strength which should be necessary to prevent the preform from deformation in the squeeze cast process. The dependence of the binder types, which were the SiO2, Al2O3, and TiO2 sols, the binder content (0-5 mass% vs whisker amount), and the sintering temperature (1000- 1 160°C) of the preform were measured. We then examined the microstructure of the obtained material which was in situ joined with the semi-solid magnesium alloy (Mg-9 mass% Al-1 mass% Zn). The results were as follows: (1) The SiO2 sol was the most effective as a binder to increase the preform compressive strength. (2) During the squeeze cast process, the particles of the α-Mg solid phase in the semi-solid alloy were filtered by the preform and piled up in front of the preform/ semi-solid alloy interface. This causes a gradational distribution of the primary crystals in part of the obtained material.
Initial results of HD radio FM trials in Brazil (2013)

This paper describes the experimental setup and presents some results of HD Radio FM digital system trials that were carried out in Brazil. The experiment is part of a series of field trials that are being conducted by the Brazilian Government to evaluate the performance of radio digital standards, which includes campaigns at MW, HF and VHF frequency bands. Analog FM and HD Radio digital signal were simultaneouslty transmitted, broadcasting the same content. The results presented here include the measurements performed in São Paulo and Belo Horizonte, Brazil.
Slow Continuous Intravenous Plasmapheresis (SCIPTM): Clinical Applications and Hemostability of Extracorporeal Ultrafiltration (2005)

An intravenous plasmapheresis catheter which excludes >99.4% of platelets from external ultrafiltration circuits is currently undergoing safety and efficacy trials for fluid removal from NYHA class II-IV congestive heart failure patients resistant to diuretic drug therapy. In animals, the SCIP catheter allowed a four fold increase in ultrafiltration efficiency without hemolysis, hemoinstability or external cartridge changes in 72 hours of treatment. Further, systemic anticoagulation was not required. These techniques might be envisioned for treatment of fluid overload in heart failure, surgery or trauma and may have applications in therapeutic apheresis, venous thrombosis, liver disease or autologous tissue engineering.
Alumina Coatings by Plasma Spraying of Monosize Sapphire Particles (1998)

Abstract In an effort to obtain a series of plasma-sprayed coatings of controlled microstructure, three monosized sapphire powders were deposited using an axial injection torch in which the plasma gas composition and nozzle diameter were the only parameters varied. The effect of these parameters on splat morphology, porosity, angular crack distribution, and hardness is reported. Uniform, dense microstructure with a hardness rivaling chromia coatings was achieved through a combination of tightly controlled processing conditions and the use of mono-disperse precursor powders.
Evolution of Renal Function after Partial and Full Mechanical Support for Chronic Heart Failure (2014)

We observed a significant improvement in renal function in patients supported by full or partial support devices, even if the preoperative renal function was severly impaired. The use of diuretics decreased in both groups. In chronic heart failure patients with impaired renal function, partial support is sufficient to improve renal function significantly.
RadioWeaves for Extreme Spatial Multiplexing in Indoor Environments (2020)

With the advances in virtual and augmented reality, gaming applications, and entertainment, certain indoor scenarios will require vastly higher capacity than what can be delivered by 5G. In this paper, we focus on massive MIMO for indoor environments. We provide a case study of the distributed deployment of the antenna elements over the walls of a room and not restricting the antenna separation to be half the wavelength. This is a new paradigm of massive MIMO antenna deployment, introduced in [1] under the name RadioWeaves. We investigate different antenna deployment scenarios in line of sight communication. We observe that the RadioWeaves deployment can spatially separate users much better than a conventional co-located deployment, which outweighs the losses caused by grating lobes and thus saves a lot on transmit power. Through simulations, we show that the RadioWeaves technology can provide high rates to multiple users by spending very little power at the transmitter compared to a co-located deployment.
Romano-Ward long QT syndrome: identification of a HERG mutation in a Taiwanese kindred. (1999)

Romano-Ward syndrome is an autosomal dominant long-QT syndrome (LQTS) that predisposes affected individuals to sudden death from tachyarrhythmias. We investigated the molecular basis of LQTS in a Taiwanese kindred. Clinical and genetic analyses revealed that a mutation was linked to the human ether-a-go-go-related gene (HERG). The coding sequences and exon-intron borders of HERG were amplified by means of polymerase chain reaction and subjected to single-strand conformation polymorphism (SSCP) analysis. An exon with an aberrant SSCP pattern was cloned and sequenced to study the molecular lesion. A C-->T transition in codon 614, leading to substitution of a valine for an alanine residue in the pore region of the HERG protein, was identified. Analysis with Bsp12861 endonuclease digestion showed the mutation to be present in all affected family members. Given that an unaffected paternal uncle had inherited the same allele from the grandfather as the proband's father, a de novo mutation had apparently occurred in the father and was transmitted to his offspring. In addition to offering presymptomatic genetic diagnosis, identification of the disease-causing mutation may suggest new therapeutic approaches for treatment and prevention of this cardiovascular disease.
Characterization of avian influenza viruses. Designation of a newly recognized haemagglutinin. (1976)

Studies with specific antisera to the haemagglutinin and neuraminidase antigens of all the influenza A subtypes show that A/turkey/Wisconsin/66 influenza virus, originally included in the Hav6 subtype, does not react in either haemagglutinin inhibition or immunodiffusion tests with antisera to Hav6. It is therefore proposed that A/turkey/Wisconsin/66 be placed in a new subtype designated Hav9. The neuraminidase antigens of the Hav6 subtype were further characterized and were shown to be N1, N2, Neq2, and Nav5 subtypes. Hav6 influenza viruses isolated from turkeys over an 11-year period showed little antigenic drift. The haemagglutinin and neuraminidase of A/shearwater/Australia/72 (Hav6Nav5) were identical with those of a virus isolated 8 years previously from a turkey in California: A/turkey/California/64 (Hav6Nav5).
AlN/GaN double-barrier resonant tunneling diodes grown by rf-plasma-assisted molecular-beam epitaxy (2002)

AlN/GaN double-barrier resonant tunneling diodes (DB–RTDs) were fabricated on (0001) Al2O3 substrates by molecular-beam epitaxy, using a rf-plasma nitrogen source. The AlN/GaN DB–RTDs were designed to have a 3-ML-thick GaN quantum well and 4-ML-thick AlN barrier layers sandwiched by Si-doped n-type GaN contact layers. The current–voltage characteristics of mesa diode samples showed clear negative differential resistance (NDR) at room temperature. The NDR was observed at 2.4 V with a peak current of 2.9 mA, which corresponds to 180 A/cm2. A peak-to-valley current ratio as high as 32 was obtained.
Constructive and computational aspects of cryptographic pairings (2009)

The security of many public-key cryptosystems relies on the existence of groups in which the discrete logarithm problem (DLP) is infeasible. Subgroups of the Jacobian varieties of elliptic and hyperelliptic curves over finite fields are widely used to realize such cryptosystems. On these groups, it is possible to define pairings. A cryptographic pairing is a bilinear, non-degenerate map that can be computed efficiently. It maps a pair of points in the Jacobian variety into the multiplicative group of a finite field. Pairings were first used in cryptography to attack the DLP on a supersingular elliptic curve by reducing it to the DLP in a finite field that is easier to solve. Later on, they led to a variety of constructive applications. When aiming at practical implementation of pairings, there are two main problems arising: The first is to find pairing-friendly curves which allow an efficient pairing computation. The second is to make computations more efficient and suitable for different applications. This dissertation addresses aspects of both problems and advances the state of the art in the associated research areas. An important condition for a pairing-friendly curve is to have an embedding degree that is small enough. Curves with this property are rare and need to be constructed. We give a method to construct pairing-friendly elliptic curves with embedding degree 12. The proposed curves have many nice properties favoring very efficient implementation, such as a prime order group of rational points over the ground field and a twist of degree 6. The Jacobian group order of a pairing-friendly curve must have a large prime divisor which satisfies the embedding degree condition. It is therefore necessary to first fix the group order and then construct the curve. As an essential tool for the construction, one uses the complex multiplication (CM) method. We show how to use the CM method to construct curves of genus 2 with p-rank 1. If pairings need to be implemented on devices with restricted memory, it may be interesting to compute pairings in compressed form. Using the fact that pairing values are elements of algebraic tori, they can be represented in a more efficient way, requiring less storage space than general field elements. We show how to do pairing computation in a compressed form. On curves with a twist of degree 6 the proposed variant of Miller’s algorithm can be done without any field inversions. Recently, it has been shown, that in many cases the elliptic curve group law can be implemented most efficiently using Edwards curves. It was an open problem to find advantageous formulas for pairing computation on Edwards curves. We state a geometric interpretation of the group law on twisted Edwards curves, give the corresponding functions, and show how to use them to compute pairings on Edwards curves. We present explicit formulas for the doubling and addition steps in Miller’s algorithm that are more efficient than all previously proposed formulas for pairings on Edwards curves and are competitive with formulas for pairing computation on Weierstraß curves.
Masksembles for Uncertainty Estimation (2021)

Deep neural networks have amply demonstrated their prowess but estimating the reliability of their predictions remains challenging. Deep Ensembles are widely considered as being one of the best methods for generating uncertainty estimates but are very expensive to train and evaluate.MC-Dropout is another popular alternative, which is less expensive, but also less reliable. Our central intuition is that there is a continuous spectrum of ensemble-like models of which MC-Dropout and Deep Ensembles are extreme examples. The first one uses effectively infinite number of highly correlated models while the second one relies on a finite number of independent models.To combine the benefits of both, we introduce Masksembles. Instead of randomly dropping parts of the network as in MC-dropout, Masksemble relies on a fixed number of binary masks, which are parameterized in a way that allows to change correlations between individual models. Namely, by controlling the overlap between the masks and their size one can choose the optimal configuration for the task at hand. This leads to a simple and easy to implement method with performance on par with Ensembles at a fraction of the cost. We experimentally validate Masksembles on two widely used datasets, CIFAR10 and ImageNet.
Decreased efficiency of MSH6 mRNA polyadenylation linked to a 20-base-pair duplication in Lynch syndrome families (2012)

International audience
ChemInform Abstract: α,α′‐BIS(PHENYLTHIO)CARBONYLS IN ORGANIC SYNTHESIS. APPLICATIONS IN THE INDOLE SERIES (1983)

Abstract Die Bis‐(phenylmercapto)‐ester (bzw. ‐lactame) (III) [erhalten durch eine Michael‐Addition des Esters (II) an die entsprechenden aktivierten Olefine], (Va) [erhalten durch Sulfenylierung des Lactams (IV)], (Vb) [erhalten durch Cyclisierung des Kondensationsprodukts (VII) von Tryptamin (VI) mit (IIIa) (R3: ‐CHO)] sowie das (nicht isolierte) Tris‐(phenylmercapto)‐acetonitril (X) werden von überschüssigen (in Gegenwart einer katalytischen Menge NaH erzeugten) Thiophenoxid‐Ionen zu den Mono‐(phenylmercapto)‐Derivaten (VIII), (XIIIa) und (XIIIb) bzw. dem Bis‐(phenylmercapto)‐acetonitril (XI) desulfenyliert [(XIIIa) und (XIIIb) werden über die entsprechenden Sulfoxide thermisch in die Enone (XIV) übergeführt; (XI) gibt mit Acrylnitril das Michael‐Addukt (XII)].
Chromatin-Remodeling Complex SWI/SNF Controls Multidrug Resistance by Transcriptionally Regulating the Drug Efflux Pump ABCB1 (2016)

Anthracyclines are among the most effective yet most toxic drugs used in the oncology clinic. The nucleosome-remodeling SWI/SNF complex, a potent tumor suppressor, is thought to promote sensitivity to anthracyclines by recruiting topoisomerase IIa (TOP2A) to DNA and increasing double-strand breaks. In this study, we discovered a novel mechanism through which SWI/SNF influences resistance to the widely used anthracycline doxorubicin based on the use of a forward genetic screen in haploid human cells, followed by a rigorous single and double-mutant epistasis analysis using CRISPR/Cas9-mediated engineering. Doxorubicin resistance conferred by loss of the SMARCB1 subunit of the SWI/SNF complex was caused by transcriptional upregulation of a single gene, encoding the multidrug resistance pump ABCB1. Remarkably, both ABCB1 upregulation and doxorubicin resistance caused by SMARCB1 loss were dependent on the function of SMARCA4, a catalytic subunit of the SWI/SNF complex. We propose that residual SWI/SNF complexes lacking SMARCB1 are vital determinants of drug sensitivity, not just to TOP2A-targeted agents, but to the much broader range of cancer drugs effluxed by ABCB1. Cancer Res; 76(19); 5810-21. ©2016 AACR.
Do genetic factors protect for early onset lung cancer? A case control study before the age of 50 years (2008)

Smoking is the most important risk factor for young lung cancer patients. However, this study provides some support for the T-Allel of GPX1(Pro200Leu) and the C-Allele of EPHX1(His113Tyr) to play a protective role in early onset lung cancer susceptibility.
Supercritical fluid technology: a sustainable and scalable approach for advanced material design and recycling (2018)

Invited talk
LA CULTURA DEL MACHO CABRÍO (BODE ) Y EL DESARROLLO LOCAL. La invención de Cabaceiras (Brasil) como ciudad turística (2014)

Goat´s Culture and Locals Development: The Invention of Cabaceiras-PB (Brazil) as a Touristi Town. Tourism is a growing phenomenon that contributes to reconstruct territories and transform local cultures. To understand these processes, this study investigated factors that contributed to the invention of Cabaceiras Paraíba (Brasil) as a tourist city, from the culture of the goat. The research was done through a qualitative approach and had the instrument for data collection semistructured interview, applied to 16 social agents of the city studied. Data were analyzed through content analysis. The results indicate that the regional culture served to generate a positive image of the city. Through the goat and the party, local agents, partnerships with government, private and third sector reinvent the city. Based on their weaknesses - too much sun, too much goats, lots of stone and little rain - Cabaceiras rebuild his image, becoming tourist town. There are, however, obstacles to overcome, because issues related to local government have contributed to the discontinuity in the execution of the project as well as the limited participation of local people in this process, agreeing to the disbelief of the population in other possible development models.
Identification of wild collected mosquito vectors of diseases using gas chromatography–mass spectrometry in Jazan Province, Saudi Arabia (2013)

Thirty-three species of mosquitoes have been reported from the Kingdom of Saudi Arabia. Several of these mosquitoes, Anopheles gambiae Giles s.l., Anopheles stephensi Liston, Culex pipiens Linnaeus, Culex quinquefasciatus Say, Culex tritaeniorhynchus Giles, Stegomyia aegypti (Linnaeus) and Aedimorphus vexans arabiensis (Patton) are known vectors of human and animal diseases. In this study, the cuticular hydrocarbon profiles of eight mosquito species using gas chromatography-mass spectrometry were analyzed. Wild collected fourth-instar larvae were reared, and single, newly emerged, unfed adult females were used for the analysis. A total of 146-160 peaks were detected from the cuticular extracts by gas chromatography. Repeated analysis of variance (ANOVA) and Tukey HSD Post Hoc test was used to test for quantitative differences in relative hydrocarbon quantity. In addition, a linear regression model was applied using Enter method to determine the diagnostic peaks for the eight mosquito specimens. The ANOVA test indicated that relative peaks were significant (P < 0.05) when selected pairs of peaks were compared. Also, seven compounds showed qualitative differences among the five mosquito vectors tested. The classes of constituents present were n-alkanes, monomethylalkanes, dimethylalkanes, trimethylalkanes, alkenes, branched aromatic hydrocarbons, aldehydes and esters. These compounds have a carbon chain length ranging from 8 to 18 carbons. The most abundant compound in all adult mosquito specimens was n-hexylacrylate [retention time (RT) 6.73 min], which was not detected in Cx. pipiens. In Cx. pipiens, the most abundant peak was benzaldehyde (RT 2.98 min). Gas chromatography-mass spectrometry is a suitable method to identify adult mosquitoes, especially from focal areas of public health concern such as Jazan Province, Saudi Arabia. This method allows a wide range of adult collected material to be identified with high accuracy.
Lipopolysaccharides, but not Angiotensin ll, lnduces Direct Pro‐lnflammatory Effects in Cultured Mouse Arteries and Human Endothelial and Vascular Smooth Muscle Cells (2016)

Abstract Angiotensin II (Ang II ) might induce pro‐inflammatory effects directly in the vascular wall independently of its haemodynamic effects. The aim of our study was to investigate the putative direct pro‐inflammatory and vasomotor effects of Ang II and compare to those of lipopolysaccharides ( LPS ) in mouse isolated mesenteric resistance‐sized arteries ( MRA ) supported by experiments in cultured human primary endothelial and vascular smooth muscle cells. Results showed that 24‐hr organ culture of mouse MRA with 10 nM Ang II had, unlike 100 ng/mL LPS , no effects on IL ‐6 or MCP ‐1 secretion, VCAM 1 mRNA expression or endothelial function, while Ang II significantly decreased maximal vasomotor responses to phenylephrine. In support, 24‐hr organ culture of mouse MRA significantly suppressed Agtr1a mRNA and augmented Tlr4 mRNA along with attenuated vasomotor responses to Ang II . Moreover, contrary to LPS and TNF ‐α, Ang II and [Sar1]‐Ang II had no concentration‐ or time‐dependent effects on IL ‐6 and MCP ‐1 secretion in human umbilical vein endothelial cells ( HUVEC ) and human aortic smooth muscle cells ( HASMC ). AGTR 1 or AGTR 2 mRNA expression was undetectable in HUVEC , whereas HASMC expressed only AGTR 1 mRNA . In summary, contrary to previous studies and the observed effects of LPS , we could not demonstrate direct vascular pro‐inflammatory effects of Ang II ex vivo or in vitro . As indicated by our results, down‐regulation or desensitization of AT 1 R during culture may explain our findings.
Live Imaging Reveals the Cellular Events Downstream of SARM1 Activation (2021)

Abstract SARM1 is an inducible NAD + hydrolase that triggers axon loss and neuronal cell death in the injured and diseased nervous system. While SARM1 activation and enzyme function are well defined, the cellular events downstream of SARM1 activity but prior to axonal demise are much less well understood. Defects in calcium, mitochondria, ATP, and membrane homeostasis occur in injured axons, but the relationships among these events have been difficult to disentangle because prior studies analyzed large collections of axons in which cellular events occur asynchronously. Here we used live imaging with single axon resolution to investigate the cellular events downstream of SARM1 activity. Our studies support a model in which SARM1 NADase activity leads to an ordered sequence of events from loss of cellular ATP, to defects in mitochondrial movement and depolarization, followed by calcium influx, externalization of phosphatidylserine, and loss of membrane permeability prior to catastrophic axonal self-destruction.
A beam-forming network for 5G systems based on precise optical clock and phase shifting (2016)

A novel, power-efficient optical beam-forming network is proposed exploiting wavelength-division-multiplexing and phase-shifting. A mode-locked laser acts as both laser comb and local oscillator. Integrated frequency-selective phase shifters perform precise beam steering of a modulated signal.