gebhart/patrepeval huggingface dataset:
MP92-06 LDL CHOLESTEROL COMPROMISES EFFICACY OF MULTITARGETED TYROSINE KINASE INHIBITORS AGAINST RENAL CELL CARCINOMA (2016)

You have accessJournal of UrologyKidney Cancer: Basic Research & Pathophysiology III1 Apr 2016MP92-06 LDL CHOLESTEROL COMPROMISES EFFICACY OF MULTITARGETED TYROSINE KINASE INHIBITORS AGAINST RENAL CELL CARCINOMA Sei Naito, Petr Makhov, Vladimir Kolenko, Robert Uzzo, and Norihiko Tsuchiya Sei NaitoSei Naito More articles by this author , Petr MakhovPetr Makhov More articles by this author , Vladimir KolenkoVladimir Kolenko More articles by this author , Robert UzzoRobert Uzzo More articles by this author , and Norihiko TsuchiyaNorihiko Tsuchiya More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.2639AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Current targeted molecular strategies, including multitargeted tyrosine kinase inhibitors (TKIs), improved survival of renal cell carcinoma (RCC) patients, the treatment responses of TKIs are not long-standing and their disease progress in most of the cases. Moreover, about one quarter of the RCC patients are primarily refractory to treatment with TKIs. We have previously demonstrated that compensatory PI3K/AKT pathway signaling promotes resistance to TKIs in genitourinary tumors. Previous studies described that Low-density lipoprotein (LDL) activates Akt/mTOR pathway in endothelial progenitor and breast cancer cells. We examined LDL roles in renal cancer cell lines, especially for TKIs resistance. METHODS We examined Akt/mTOR pathway activation using by Western blot analysis. The cell viability inhibition by some drugs with/without LDL were examined in two RCC cell lines (SK-45 and HRC-31) using by CellTiter Blue® assay. The LDL effects were verified on RCC cell line (PNX-0010) xenograft rodent model. RESULTS LDL promptly phosphorylated Akt in dose-dependent manner (Figure 1), and prevented the viability inhibitions induced by TKIs. The LDL-inducible prevention for sorafenib was restored by Akt inhibitor (Figure 2). The PNX-0010 xenograft rodent model with high serum LDL level showed sunitinib resistancy (Figure 3). CONCLUSIONS We demonstrate here that the addition of LDL cholesterol increases AKT phosphorylation and, importantly, compromises the cytotoxicity of multiple clinically relevant TKIs in a cell model of human RCC. Furthermore, RCC patient-derived xenografts resisted TKIs in mice fed a high fat/high cholesterol diet. Our results delineate a clinically useful strategy of lowering blood cholesterol to overcome the TKI resistance in cholesterol-addicted RCC tumors. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e1163-e1164 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Sei Naito More articles by this author Petr Makhov More articles by this author Vladimir Kolenko More articles by this author Robert Uzzo More articles by this author Norihiko Tsuchiya More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...
Centroid Acquisition (2016)

This document contains recommendations for terminology in mass spectrometry. Development of standard terms dates back to 1974 when the IUPAC Commission on Analytical Nomenclature issued recommendations on mass spectrometry terms and definitions. In 1978, the IUPAC Commission on Molecular Structure and Spectroscopy updated and extended the recommendations and made further recommendations regarding symbols, acronyms, and abbreviations. The IUPAC Physical Chemistry Division Commission on Molecular Structure and Spectroscopy’s Subcommittee on Mass Spectroscopy revised the recommended terms in 1991 and appended terms relating to vacuum technology. Some additional terms related to tandem mass spectrometry were added in 1993 and accelerator mass spectrometry in 1994. Owing to the rapid expansion of the field in the intervening years, particularly in mass spectrometry of biomolecules, a further revision of the recommendations has become necessary. This document contains a comprehensive revision of mass spectrometry terminology that represents the current consensus of the mass spectrometry community.
Prevalence of jaw osteonecrosis among patients receiving bisphosphonates in Riyadh (2011)

The objective of this study is to obtain data on the prevalence of bisphosphonate-associated osteonecrosis of the jaws (ONJ) among patients who received dental treatment in Riyadh. The study was conducted in five hospitals in the Riyadh area. All subjects were interviewed to collect data regarding their medical history, bisphosphonates usage, dental history, and possible complications after dental treatment. A total of 88 subjects under bisphosphonates therapy were interviewed, 25% were males and 75% were females. Bisphosphonates were taken orally by 89.7% of the subjects while 10.2% received bisphosphonate intravenously. All the subjects have had dental treatments after the beginning of bisphosphonate therapy. 79.5% of those patients had dental extraction, 13.6% root canal treatment, 4.5% oral surgery, and 4.5% received dental implants. No complications were reported after the dental treatment. In the present study, patients with bisphosphonates therapy did not develop complication after the dental treatment. However, it is recommended that patients receiving bisphosphonate therapy should be treated with precaution. Dental awareness programs should be provided to the physicians prescribing bisphosphonates to their patients.
Human Embryonic Stem Cell-Derived Mesenchymal Stem Cell Seeding on Calcium Phosphate Cement-Chitosan-RGD Scaffold for Bone Repair (2012)

Calcium phosphate cement (CPC) has in situ-setting ability and excellent osteoconductivity. Human embryonic stem cells (hESCs) are exciting for regenerative medicine due to their strong proliferative ability and multilineage differentiation capability. However, there has been no report on hESC seeding with CPC. The objectives of this study were to obtain hESC-derived mesenchymal stem cells (hESCd-MSCs), and to investigate hESCd-MSC proliferation and osteogenic differentiation on novel CPC with chitosan immobilized with RGD (CPC-chitosan-RGD). RGD was covalently bonded with chitosan, which was then incorporated into CPC. The CPC-chitosan-RGD scaffold had higher strength and toughness than CPC-chitosan control without RGD (p<0.05). hESCs were cultured to form embryoid bodies (EBs), and the MSCs were then migrated out of the EBs. Flow cytometry indicated that the hESCd-MSCs expressed typical surface antigen profile of MSCs. hESCd-MSCs had good viability when seeded on CPC scaffolds. The percentage of live cells and the cell density were significantly higher on CPC-chitosan-RGD than CPC-chitosan control. Scanning electron microscope examination showed hESCd-MSCs with a healthy spreading morphology adherent to CPC. hESCd-MSCs expressed high levels of osteogenic markers, including alkaline phosphatase, osteocalcin, collagen I, and Runx2. The mineral synthesis by the hESCd-MSCs on the CPC-chitosan-RGD scaffold was twice that for CPC-chitosan control. In conclusion, hESCs were successfully seeded on CPC scaffolds for bone tissue engineering. The hESCd-MSCs had good viability and osteogenic differentiation on the novel CPC-chitosan-RGD scaffold. RGD incorporation improved the strength and toughness of CPC, and greatly enhanced the hESCd-MSC attachment, proliferation, and bone mineral synthesis. Therefore, the hESCd-MSC-seeded CPC-chitosan-RGD construct is promising to improve bone regeneration in orthopedic and craniofacial applications.
Peritoneal Cell-free DNA: an innovative method for determining acute cell damage in peritoneal membrane and for monitoring the recovery process after peritonitis (2015)

Our results demonstrate that cfDNA increases in peritoneal effluent of PD patients with peritonitis and tends to progressively decline in step with peritonitis resolution and membrane repair process. Peritoneal cfDNA quantification could be an innovative method to determine acute damage and an inverse index of the repair process.
Skillometers (2013)

Applications typically provide ways for expert users to increase their performance, such as keyboard shortcuts or customization, but these facilities are frequently ignored. To help address this problem, we introduce skillometers -- lightweight displays that visualize the benefits available through practicing, adopting a better technique, or switching to a faster mode of interaction. We present a general framework for skillometer design, then discuss the design and implementation of a real-world skillometer intended to increase hotkey use. A controlled experiment shows that our skillometer successfully encourages earlier and faster learning of hotkeys. Finally, we discuss general lessons for future development and deployment of skillometers.
Uteroglobin Binding Proteins: Regulation of Cellular Motility and Invasion in Normal and Cancer Cells (2000)

Uteroglobin (UG) is a multifunctional, secreted protein with anti-inflammatory and antichemotactic properties. While its anti-inflammatory effects, in part, stem from the inhibition of soluble phospholipase A2 (sPLA2) activity, the mechanism(s) of its antichemotactic effects is not clearly understood. Although specific binding of UG on microsomal and plasma membranes has been reported recently, how this binding affects cellular function is not clear. Here, we report that recombinant human UG (hUG) binds to both normal and cancer cells with high affinity (20-35 nM, respectively) and specificity. Affinity cross-linking studies revealed that 125I-hUG binds to the NIH 3T3 cell surface with two proteins of apparent molecular masses of 190 and 49 kDa, respectively. UG affinity chromatography yielded similar results. While both the 190- and 49-kDa proteins were expressed in the heart, liver, and spleen, the lung and trachea expressed only the 190-kDa protein. Some cancer cells (e.g., mastocytoma, sarcoma, and lymphoma) expressed both the 190- and 49-kDa proteins. Further, using functional assays, we found that UG dramatically suppressed the motility and extracellular matrix invasion of both NIH 3T3 and some cancer cells. In order to further characterize the anti-ECM-invasive properties of UG, we induced expression of hUG into cancer cell lines derived from organs that, under physiological circumstances, secrete UG at a high level. Interestingly, it has been reported that a high percentage of the adenocarcinomas arising from the same organs fail to express UG. Our results on induced hUG expression in these cells show that inhibition of motility and ECM invasion requires the expression of both UG and its binding proteins. Taken together, our data define receptor-mediated functions of UG in which this protein regulates vital cellular functions by both autocrine and paracrine pathways.
Dissecting the molecular signatures underlying hybrid oncocytic tumors in Birt-Hogg-Dubé syndrome. (2023)

707 Background: Birt-Hogg-Dubé (BHD) syndrome is associated with an increased risk of renal tumors including the hybrid oncocytic tumors (HOT), renal oncocytoma (RO) and chromophobe renal cell carcinoma (CHRCC). HOT are known to exhibit morphological features overlapping between RO and CHRCC. The molecular underpinnings of HOT and the corresponding morphologic correlates remain poorly understood. Herein we aimed to understand the distinct molecular features underlying HOT and how the genomic phenotype differs from CHRCC. Methods: We performed next generation sequencing and single cell sequencing analysis to understand the clinico-pathologic aspects and tumor biology of HOT and CHRCC, including tumor development. This was followed by spatial transcriptome analysis of the distinct HOT tumor compartments, using nanoString GeoMX digital spatial profiler. The candidate biomarker expression was confirmed by immunohistochemistry (IHC) and RNA in situ hybridization (RNA-ISH), a unique gene expression visualization molecular technology. Results: We discovered unique gene expression signatures underlying the two distinct morphological patterns seen in HOT. FOXI1 and L1CAM show a striking mutually exclusive expression pattern in the two cellular populations seen within this tumor. The signature genes of RO like cells seen within HOT resembles the principal cells of the distal nephron seen in normal kidney; and the signature genes of the CHRCC like cells resembles the intercalated cells of the distal nephron. This suggests distal nephron as the cellular site of origin for HOT. We also nominated tumor specific biomarkers for HOT. Conclusions: We identified unique gene expression signatures which are definitional for HOT and nominated candidate diagnostic biomarkers to help distinguish HOT from other renal tumor subtypes with overlapping morphology. This finding would improve the diagnostic accuracy, assist familial genetic monitoring, and facilitate disease management.
P2‐323: Toll‐like receptor 9 stimulation via CpG ODN in a non‐human primate model of sporadic cerebral amyloid angiopathy (2015)

Immunomodulation is a promising therapeutic approach for Alzheimer's disease (AD); however, major drawbacks are cerebral microhemorrhages associated with increased cerebral amyloid angiopathy (CAA) and excessive inflammation. Our initial findings indicate that stimulation of TLR9 signaling with CpG oligodeoxynucleotide (ODN) is effective against CAA without inducing toxicity in AD mouse models. To further assess potential human use of CpG ODN we advanced our studies using a well-established non-human primate model of sporadic CAA, squirrel monkey (Saimiri Boliviensis). Safety and efficacy assessment studies were first performed in young squirrel monkeys (SQM). Elderly female monkeys were subcutaneously injected either with the most effective and non-toxic dosages of the class B CpG ODN containing a primate specific immunostimulatory sequence or saline. Both age groups were subjected to behavioral testing. Plasma taken during the course of treatment was analyzed to identify immune responses and AD biomarkers. Fluidigm RT-PCR was used to evaluate mRNA levels of cytokines in SQM PBMCs. CpG ODN elevated the levels of various Th1/Th2 cytokines in plasma from old monkeys. Upregulation of cytokines in CpG ODN group was further confirmed by RT-PCR. Pre-treatment behavioral assessment in our aged monkeys demonstrated cognitive deficits on the Inhibitory Control of Behavior and Delayed Response tests. Age effect on cognitive abilities was observed as the young group performed with overall lower session error rates compared to old animals. Post-treatment behavioral testing in our aged monkeys is ongoing. Here we report the first pyroglutamate (pE3) immunohistochemistry of aged Saimiri Boliviensis. In addition to 6E10/4G8 Aß-positive plaques, pyroglutamate Aß-positive deposits in the form of CAA and parenchymal plaques were detected. Our preliminary biomarker analyses revealed a noticeable increase in Aß40, Aß42 and AßpE3 plasma levels in CpG ODN-treated group. Further longitudinal assessment of potential AD biomarkers is currently in progress. The presented studies represent the first trial of specifically targeting CAA in non-human primates. We hope that our research will validate this novel approach of immunomodulation as a safer method to successfully ameliorate AD related pathologies and provide critical data for potential clinical use of CpG ODN in AD patients.
MONITORING AND CONTROL OF REFRACTORY WEAR FOR INTENSIVE OPERATION OF BLAST FURNACE (2017)

One of the main reasons for blast furnace reline is the loss of refractory lining, particularly in the hearth and taphole regions. Intense operation of the blast furnace can result in extensive refractory wear and deterioration. This can then affect the continuity of the operation and the furnace campaign life. Novo-Lipetsk (NLMK) and Hatch developed a systematic approach to monitor the condition of blast furnace hearth linings using Acousto Ultrasonic-Echo (AU-E), a non-destructive testing system developed by Hatch. Continuous and periodic inspections of blast furnaces revealed areas with accelerated refractory deterioration, formation of elephant foot, extent and speed of refractory wear, as well as cracks and other anomalies. Improvement in coke quality, stave washing, the addition of titania, and other changes were recommended and implemented to prolong furnace life while maintaining the intensity of furnace operation. This paper demonstrates how the coordinated efforts between NLMK and Hatch resulted in prolonging the blast furnace campaign life.
NOVEL OBSERVATION ON BASE-CATALYZED BENZOYLATION OF DIALKYL PHOSPHORANILIDATES (1987)

Abstract N-Benzoylated dialkyl phosphoranilidates undergo stereoretentive rearrangement to (N-phenylimino) benzylidynoxy derivatives. These derivatives hydrolyse in pyridine exclusively via attack by water at N-phenylimino carbon.
Probing color coherence effects in pp collisions at s=7TeV (2014)

A study of color coherence effects in pp collisions at a center-of-mass energy of 7 TeV is presented. The data used in the analysis were collected in 2010 with the CMS detector at the LHC and correspond to an integrated luminosity of 36 pb -1. Events are selected that contain at least three jets and where the two jets with the largest transverse momentum exhibit a back-to-back topology. The measured angular correlation between the second- and third-leading jet is shown to be sensitive to color coherence effects, and is compared to the predictions of Monte Carlo models with various implementations of color coherence. None of the models describe the data satisfactorily.
A Smart Shape Memory Alloy Actuated Microvalve With Feedback Control (1998)

Abstract The microfluidics group at CWRU has demonstrated a thin film shape memory alloy actuated smart microvalve with feedback control for fluid control. A micromachined flow sensor was designed and fabricated and feedback control electronics were developed and incorporated with a TiNi microvalve to realize the system. The valve is a Titanium Nickel (TiNi) Shape Memory Alloy (SMA) thin film actuated micromachined valve capable of modulating 0–250 ml/min of airflow at 2 psi. The flow sensor used is a micromachined, anemometric type flow sensor. By simulation, the smart controller was designed using MATLAB with Simulink and realized with standard integrated circuits. The performance of the smart valve shows flow insensitivity to pressure variations across the valve. This was demonstrated by a constant flow even when the valve is subject to a change in the pressure drop across the valve. This is in contrast to the typical behavior of standard valves without flow sensing and feedback control.
Spatio-temporal provenance: Identifying location information from unstructured text (2013)

Spatio-temporal attributes represent two aspects of physical presence - space and time - which are integral to human activities. Space-time markers of an entity in conjunction with correlation with other networks such as movements in social network, the road/transportation network encodes a wealth of provenance information. With the advent of mobile computing and cheap and improved location estimation techniques, encoding such information has become commonplace. In this paper, we will focus on deriving such location provenance information from unstructured text generated by social media. As social media such as Facebook and Twitter are integrated with mobile devices, information generated by individuals in these networks gets tagged with spatial markers. We can classify such markers into explicit and implicit tags, where explicit tags encode the spatial data explicitly by providing the accurate location attributes. On the other hand, a lot of social network data may not encode such information explicitly. Our hypothesis in this paper is that the unstructured textual data contains implicit spatial markers at a fine granularity. We develop algorithms to support this hypothesis and evaluate these algorithms on data from FourSquare to show that the spatial category information can be identified with an accuracy of over 80%.
Proposed method for measuring the duration of electron pulses by attosecond streaking (2008)

We propose a method to measure the duration of ultrashort electron pulses. The electron pulse to be measured impinges on a solid target, causing the emission of Auger electrons through impact ionization. The energy spectrum of the Auger electrons is altered in the presence of an intense femtosecond laser field. Due to the extremely short lifetime of the Auger effect, this effect can be used to generate cross correlation between a laser and an electron pulse. The method is applicable to electron pulses ranging from hundreds of attoseconds to hundreds of femtoseconds in duration, and for a few hundreds of electron volts to relativistic energies.
The original Lujan syndrome family has a novel missense mutation (p.N1007S) in the MED12 gene (2007)

A novel missense mutation in the mediator of RNA polymerase II transcription subunit 12 (MED12) gene has been found in the original family with Lujan syndrome and in a second family (K9359) that was initially considered to have Opitz-Kaveggia (FG) syndrome. A different missense mutation in the MED12 gene has been reported previously in the original family with FG syndrome and in five other families with compatible clinical findings. Neither sequence alteration has been found in over 1400 control X chromosomes. Lujan (Lujan-Fryns) syndrome is characterised by tall stature with asthenic habitus, macrocephaly, a tall narrow face, maxillary hypoplasia, a high narrow palate with dental crowding, a small or receding chin, long hands with hyperextensible digits, hypernasal speech, hypotonia, mild-to-moderate mental retardation, behavioural aberrations and dysgenesis of the corpus callosum. Although Lujan syndrome has not been previously considered to be in the differential diagnosis of FG syndrome, there are some overlapping clinical manifestations. Specifically, these are dysgenesis of the corpus callosum, macrocephaly/relative macrocephaly, a tall forehead, hypotonia, mental retardation and behavioural disturbances. Thus, it seems that these two X-linked mental retardation syndromes are allelic, with mutations in the MED12 gene.
DEVELOPMENT OF A MULTIPLEX QUANTITATIVE PCR SIGNATURE TO PREDICT POOR OUTCOME IN BLADDER CANCER (2008)

You have accessJournal of Urology1 Apr 2008DEVELOPMENT OF A MULTIPLEX QUANTITATIVE PCR SIGNATURE TO PREDICT POOR OUTCOME IN BLADDER CANCER Rou Wang, Alon Z Weizer, David S Morris, Scott A Tomlins, Robert J Lonigro, Alexander Tsodikov, Cheryl T Lee, and Arul M Chinnaiyan Rou WangRou Wang More articles by this author , Alon Z WeizerAlon Z Weizer More articles by this author , David S MorrisDavid S Morris More articles by this author , Scott A TomlinsScott A Tomlins More articles by this author , Robert J LonigroRobert J Lonigro More articles by this author , Alexander TsodikovAlexander Tsodikov More articles by this author , Cheryl T LeeCheryl T Lee More articles by this author , and Arul M ChinnaiyanArul M Chinnaiyan More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(08)60918-0AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "DEVELOPMENT OF A MULTIPLEX QUANTITATIVE PCR SIGNATURE TO PREDICT POOR OUTCOME IN BLADDER CANCER." The Journal of Urology, 179(4S), p. 314 © 2008 by American Urological AssociationFiguresReferencesRelatedDetails Volume 179Issue 4SApril 2008Page: 314 Advertisement Copyright & Permissions© 2008 by American Urological AssociationMetricsAuthor Information Rou Wang More articles by this author Alon Z Weizer More articles by this author David S Morris More articles by this author Scott A Tomlins More articles by this author Robert J Lonigro More articles by this author Alexander Tsodikov More articles by this author Cheryl T Lee More articles by this author Arul M Chinnaiyan More articles by this author Expand All Advertisement PDF downloadLoading ...