gebhart/patrepeval huggingface dataset:
Mikrowellen-unterstützte Herstellung von TiO2 und Gd2O3 Nanopartikeln und deren Charakterisierung (2006)

Zur Herstellung von nanopartikulären keramischen Pulvern verschiedener Oxide, wie TiO2 und Gd2O3, wurde eine veränderte mikrowellenunterstützte Polyolmethode (MAP) verwendet. Aufgrund der guten dielektrischen Eigenschaften der verwendeten Lösungsmittel, wie Diethylenglykol und 1,4 Butandiol, ist Mikrowellenheizen möglich, wodurch eine bedeutende Reduzierung der Reaktionszeit erhalten wird. Die Pulver haben eine mittlere Primärpartikelgröße von < 5 nm. Einflüsse auf die Partikelgröße und -verteilung sowie die Porengrößenverteilung und Oberflächengröße wurden durch Variation des Prekursors, dessen Konzentration im Lösungsmittel, der zugegebenen Menge an Wasser oder Hydroxid, des pH-Werts der Lösung und der MW-Parameter untersucht. Pulver, die über diese Route hergestellt wurden, zeigen die Eigenschaft, Polyol in die molekulare Struktur zu interkalieren. Diese Besonderheit, über die auch eine japanische Gruppe berichtet, wird ebenso beleuchtet und dürfte neue technische Anwendungsfelder eröffnen. Die allgemeine Anwendbarkeit dieser Methode und deren Vorteile bezüglich Eigenschaften der Pulver und Verarbeitbarkeit werden gezeigt. Die Pulver wurden mittels Röntgenbeugung, Elektronenmikroskopie und Physisorption untersucht.
A Systematic Review of Solid-Pseudopapillary Neoplasms (2014)

The number of SPNs reported in the literature has seen a 7-fold increase in the number of cases reported since 2000 compared with before. Solid-pseudopapillary neoplasms continue to be primarily found in young women and present with nonspecific symptoms. Surgery remains the mainstay of treatment with an excellent long-term prognosis.
Macrophage-inactivating IL-13 suppresses experimental autoimmune encephalomyelitis in rats. (1994)

Experimental autoimmune encephalomyelitis (EAE) is initiated by myelin basic protein (MBP)-specific CD4+ T cells of the Th1 phenotype that subsequently trigger the invasion of monocytes/macrophages into the brain. In this study, we evaluated the potential of human recombinant (hr) IL-13 to exert a protective effect on the development of EAE in Lewis rats. hrIL-13 is found to be a potent in vitro modulator of various rat macrophage functions, including an inhibition of the production of the proinflammatory cytokines IL-1 beta and TNF, and a simultaneous enhancement of MHC class II and CD4 receptor expression. Furthermore, hrIL-13 displayed a slight, but highly reproducible, inhibitory effect on the in vitro proliferative responses of encephalitogenic MBP-specific T cells stimulated in the presence of thymic APCs. Upon in vivo application of hrIL-13-secreting vector cells into MBP-immunized animals, the cytokine was capable of markedly suppressing the development of EAE, as assessed by a reduction of the mean duration, severity, and incidence of disease. This suppression of disease coincided with an only minimal reduction of MBP-directed T cell autoreactivity and no alteration in MBP-specific autoantibody production. We infer from these results that a strictly Th1-initiated immune disease can be attenuated efficiently by the administration of a cytokine that primarily targets cells of the macrophage/monocyte lineage and seems to exert no undesirable general suppression on either T cell or B cell immunoreactivity in vivo.
Mycobacterium tuberculosis Eis protein initiates suppression of host immune responses by acetylation of DUSP16/MKP-7 (2012)

The intracellular pathogen Mycobacterium tuberculosis (Mtb) causes tuberculosis. Enhanced intracellular survival (Eis) protein, secreted by Mtb, enhances survival of Mycobacterium smegmatis (Msm) in macrophages. Mtb Eis was shown to suppress host immune defenses by negatively modulating autophagy, inflammation, and cell death through JNK-dependent inhibition of reactive oxygen species (ROS) generation. Mtb Eis was recently demonstrated to contribute to drug resistance by acetylating multiple amines of aminoglycosides. However, the mechanism of enhanced intracellular survival by Mtb Eis remains unanswered. Therefore, we have characterized both Mtb and Msm Eis proteins biochemically and structurally. We have discovered that Mtb Eis is an efficient N(ε)-acetyltransferase, rapidly acetylating Lys55 of dual-specificity protein phosphatase 16 (DUSP16)/mitogen-activated protein kinase phosphatase-7 (MKP-7), a JNK-specific phosphatase. In contrast, Msm Eis is more efficient as an N(α)-acetyltransferase. We also show that Msm Eis acetylates aminoglycosides as readily as Mtb Eis. Furthermore, Mtb Eis, but not Msm Eis, inhibits LPS-induced JNK phosphorylation. This functional difference against DUSP16/MKP-7 can be understood by comparing the structures of two Eis proteins. The active site of Mtb Eis with a narrow channel seems more suitable for sequence-specific recognition of the protein substrate than the pocket-shaped active site of Msm Eis. We propose that Mtb Eis initiates the inhibition of JNK-dependent autophagy, phagosome maturation, and ROS generation by acetylating DUSP16/MKP-7. Our work thus provides insight into the mechanism of suppressing host immune responses and enhancing mycobacterial survival within macrophages by Mtb Eis.
Hole-Burning Studies on Triplet-Triplet Fluorescence Excitation Spectrum of 2-Naphthylphenylcarbene (1996)

Abstract Spectral holes were burned on the triplet-triplet 0-0 fluorescence excitation lines of main conformers of 2-naphthylphenylcarbene in n-hexane and n-heptane at 1.8 K. The central holes, at a frequency of laser burning light have usually several accompanying satellite holes in a range of 1.5 GHz around and deep holes on the low energy side, 14.5–16.5 GHz away. Such a pattern of holes reflects the zero field splitting of the ground T0 and first excited T1 triplet states and the fact that an energy relaxation by the intersystem crossing channel is highly selective.
ChemInform Abstract: Recent Advances in the Isolation and Reactivity of Organogold Complexes (2012)

Abstract Review: 63 refs.
Towards Confirming Neural Circuit Inference from Population Calcium Imaging. NIPS Workshop on Connectivity Inference in Neuroimaging (2015)

Vogelstein JT, Mishchenko Y, Packer AM, Machado TA, Yuste R, Paninski L. Towards Confirming Neural Circuit Inference from Population Calcium Imaging. NIPS Workshop on Connectivity Inference in Neuroimaging, 2009
Genetically and chemically tuned haemoglobin–albumin trimers with superior O2 transport efficiency (2021)

Haemoglobin (Hb)-albumin (HSA) trimers were synthesized using five distinct Hb variants in which the structures were genetically and chemically tuned as an artificial O<sub>2</sub> carrier and used as a red blood cell (RBC) substitute. The trimers were found to have moderately low O<sub>2</sub> affinity (<i>p</i><sub>50</sub> = 23-34 Torr, 37 °C) and high co-operativity, yielding a maximum O<sub>2</sub> transport efficiency 1.8-fold higher than that of human RBCs.
Homozygous Triplicate Mutations in Three 16S rRNA Genes Responsible for High-Level Aminoglycoside Resistance inNocardia farcinicaClinical Isolates from a Canada-Wide Bovine Mastitis Epizootic (2010)

Nocardia farcinica strains showing high-level resistance to amikacin were isolated from clinical cases in a Canada-wide bovine mastitis epizootic. Shotgun cloning of the resistance genes in the amikacin-resistant mastitis isolate N. farcinica IFM 10580 (W6220 [Centers for Disease Control and Prevention]) using a multicopy vector system revealed that the 16S rRNA gene with an A-to-G single-point mutation at position 1408 (in Escherichia coli numbering) conferred "moderate" cross-resistance to amikacin and other aminoglycosides to an originally susceptible N. farcinica strain IFM 10152. Subsequent DNA sequence analyses revealed that, in contrast to the susceptible strain, all three chromosomal 16S rRNA genes of IFM 10580, the epizootic clinical strain, contained the same A1408G point mutations. Mutant colonies showing high-level aminoglycoside resistance were obtained when the susceptible strain N. farcinica IFM 10152 was transformed with a multicopy plasmid carrying the A1408G mutant 16S rRNA gene and was cultured in the presence of aminoglycosides for 3 to 5 days. Of these transformants, at least two of the three chromosomal 16S rRNA genes contained A1408G mutations. A triple mutant was easily obtained from a strain carrying the two chromosomal A1408G mutant genes and one wild-type gene, even in the absence of the plasmid. The triple mutant showed the highest level of resistance to aminoglycosides, even in the absence of the plasmid carrying the mutant 16S rRNA gene. These results suggest that the homozygous mutations in the three 16S rRNA genes are responsible for the high-level aminoglycoside resistance found in N. farcinica isolates of the bovine mastitis epizootic.
Successful inhibition of intracranial human glioblastoma multiforme xenograft growth via systemic adenoviral delivery of soluble endostatin and soluble vascular endothelial growth factor receptor-2 (2008)

These results suggest that systemic delivery and sustained production of endostatin and soluble VEGFR2 can slow intracranial glial tumor growth by both reducing cell proliferation and increasing tumor apoptosis. This work adds further support to the concept of using antiangiogenesis therapy for intracranial GBM.
Conversion from left ventricular cylinder coordinates to radiographic projections during ECG-guided catheter ablation of cardiac arrhythmias (2002)

Electrocardiographic Body Surface Mapping can assist in procedures for catheter ablation of cardiac arrhythmias, by providing an estimate of the site of origin of an arrhythmia. The authors' localization methods provide their results in left ventricular cylinder coordinates (LVCC). It would be preferable if localization results were presented in the radiographic views, possibly superimposed on the X-ray images. This requires computation of a position on the endocardial surface of the left ventricular wall from LVCC. Perfect translation between a position on the endocardial wall and cylinder coordinates would be possible if the geometry of the wall were perfectly known. Unfortunately, little is known about the wall geometry in practice. Therefore, the authors investigated how well one can reconstruct 3D positions from LVCC with limited means, by computing LVCC from biplane radiographic images, and reconstructing the positions from the LVCC. The authors' model of the wall consisted of a half-ellipsoid, scaled to the individual ventricular length and diameter. The method was evaluated using 111 catheter positions obtained in 8 patients. The reconstruction error in 3D space was 9/spl plusmn/8% of the ventricular length. The method is therefore accurate enough to be useful during a catheter ablation procedure.
Catalytic and spectroscopic analysis of blue copper-containing nitrite reductase mutants altered in the environment of the type 2 copper centre: implications for substrate interaction (2001)

The blue dissimilatory nitrite reductase (NiR) from Alcaligenes xylosoxidans is a trimer containing two types of Cu centre, three type 1 electron transfer centres and three type 2 centres. The latter have been implicated in the binding and reduction of nitrite. The Cu ion of the type 2 centre of the oxidized enzyme is ligated by three His residues, and additionally has a co-ordinated water molecule that is also hydrogen-bonded to the carboxyl of Asp92 [Dodd, Van Beeumen, Eady and Hasnain (1998), J. Mol. Biol. 282, 369Ő382]. Two mutations of this residue have been made, one to a glutamic acid residue and a second to an asparagine residue; the effects of both mutations on the spectroscopic and catalytic properties of the enzyme have been analysed. EPR spectroscopy revealed that both mutants retained intact type 1 Cu centres with g‖ = 2.12 (A‖ = 0mT) and g⊥ = 2.30 (A⊥ = 6.4mT), which was consistent with their blue colour, but differed in their activities and in the spectroscopic properties of the type 2 centres. The D92E mutant had an altered geometry of its type 2 centre such that nitrite was no longer capable of binding to elicit changes in the EPR parameters of this centre. Accordingly, this mutation resulted in a form of NiR that had very low enzyme activity with the artificial electron donors reduced Methyl Viologen and sodium dithionite. As isolated, the EPR spectrum of the Asp92 → Asn (D92N) mutant showed no characteristic type 2hyperfine lines. However, oxidation with iridium hexachloride partly restored a type 2 EPR signal, suggesting that type 2 copper is present in the enzyme but in a reduced, EPR-silent form. Like the Asp92 → Glu mutant, D92N had very low enzyme activities with either Methyl Viologen or dithionite. Remarkably, when the physiological electron donor reduced azurin I was used, both mutant proteins exhibited restoration of enzyme activity. The degree of restoration differed for the two mutants, with the D92N derivative exhibiting approx. 60% of the activity seen for the wild-type NiR. These findings suggest that on formation of an electron transfer complex with azurin, a conformational change in NiR occurs that returns the catalytic Cu centre to a functionally active state capable of binding and reducing nitrite.
Modulatory effect of mannose‐binding lectin on cytokine responses: possible roles in HIV infection (2005)

The present study suggests a modulatory role of MBL on cytokine responses, and HIV replication after stimulation with microbial products. These effects of MBL on inflammatory responses and viral replication may be clinically relevant for HIV infection.
Omalizumab (Anti-IgE) Therapy Increases Blood Glucose Levels in Severe Persistent Allergic Asthma Patients with Diabetes Mellitus: 18 Month follow-up (2014)

To our knowledge, this is the first time an association between omalizumab use and hyperglycemia has been documented. Every vial of Xolair (150 mg) contains 145.5 mg sucrose and it might increase the blood levels of glucose in diabetics. As a conclusion the prescribing information might have been revised based on post marketing surveillance data and reported such cases indicating that different side effects may occur beyond 2 hours of the injection.
Results of the phase II EORTC study comparing paclitaxel/cisplatin with teniposide/cisplatin in patients with non-small cell lung cancer. EORTC Lung Cancer Cooperative Group. (1996)

Toxicity and response rates are evaluated in a randomized phase II study comparing paclitaxel (Taxol; Bristol-Myers Squibb Company, Princeton, NJ)/cisplatin with teniposide/cisplatin in the treatment of non-small cell lung cancer to decide whether this study should continue as a phase III trial. A response was seen in 26% (10 of 38) of the patients receiving teniposide/cisplatin and in 40% (14 of 35) of those receiving paclitaxel/cisplatin. Overall, evidence of toxicity was more severe in the cisplatin/teniposide group, especially with regard to myelotoxicity. Grade 4 neutropenia was seen in 66% of patients receiving teniposide compared with 29% of those treated with paclitaxel/cisplatin. The study is to continue as a phase III trial, with 150 patients expected to participate in each treatment group.
Binocular combination of phase and contrast explained by a gain-control and gain-enhancement model (2013)

We investigated suprathreshold binocular combination, measuring both the perceived phase and perceived contrast of a cyclopean sine wave. We used a paradigm adapted from Ding and Sperling (2006, 2007) to measure the perceived phase by indicating the apparent location (phase) of the dark trough in the horizontal cyclopean sine wave relative to a black horizontal reference line, and we used the same stimuli to measure perceived contrast by matching the binocular combined contrast to a standard contrast presented to one eye. We found that under normal viewing conditions (high contrast and long stimulus duration), perceived contrast is constant, independent of the interocular contrast ratio and the interocular phase difference, while the perceived phase shifts smoothly from one eye to the other eye depending on the contrast ratios. However, at low contrasts and short stimulus durations, binocular combination is more linear and contrast summation is phase-dependent. To account for phase-dependent contrast summation, we incorporated a fusion remapping mechanism into our model, using disparity energy to shift the monocular phases towards the cyclopean phase in order to align the two eyes' images through motor/sensory fusion. The Ding-Sperling model with motor/sensory fusion mechanism gives a reasonable account of the phase dependence of binocular contrast combination and can account for either the perceived phase or the perceived contrast of a cyclopean sine wave separately; however it requires different model parameters for the two. However, when fit to both phase and contrast data simultaneously, the Ding-Sperling model fails. Incorporating interocular gain enhancement into the model results in a significant improvement in fitting both phase and contrast data simultaneously, successfully accounting for both linear summation at low contrast energy and strong nonlinearity at high contrast energy.
Investigation on Optimum Radio Link Connection Using Remote Radio Equipment in Heterogeneous Network for LTE-Advanced (2009)

This paper proposes the optimum radio link connection (RLC) using remote radio equipment (RRE) in a heterogeneous network in which various types of cell configurations exist with different cell sizes and downlink transmission power levels for LTE (Long-Term Evolution)-Advanced. The instantaneous received signal-to-interference plus noise power ratio (SINR) and the user throughput performance employing the proposed RLC methods are investigated using system-level simulations. From the simulation results, we show that the proposed independent RLC method between the downlink and uplink and the proposed multipoint RLC method based on coordinated multipoint transmission/reception achieve a higher user throughput both in the downlink and uplink compared to the conventional common RLC method. Therefore, the proposed independent and multipoint RLC methods are very promising candidates to improve the user throughput particularly near the cell edge in a heterogeneous network for LTE-Advanced.
Identification of a TonB-Dependent Receptor Involved in Lanthanide Switch by the Characterization of Laboratory-Adapted Methylosinus trichosporium OB3b (2023)

Two methanol dehydrogenases (MDHs), MxaFI and XoxF, have been characterized in methylotrophic and methanotrophic bacteria. MxaFI contains a calcium ion in its active site, whereas XoxF contains a lanthanide ion. Importantly, the expression of MxaFI and XoxF is inversely regulated by lanthanide bioavailability, i.e., the "lanthanide switch." To reveal the genetic and environmental factors affecting the lanthanide switch, we focused on two Methylosinus trichosporium OB3b mutants isolated during routine cultivation. In these mutants, MxaF was constitutively expressed, but lanthanide-dependent XoxF1 was not, even in the presence of 25 μM cerium ions, which is sufficient for XoxF expression in the wild type. Genotyping showed that both mutants harbored a loss-of-function mutation in the CQW49_RS02145 gene, which encodes a TonB-dependent receptor. Gene disruption and complementation experiments demonstrated that CQW49_RS02145 was required for XoxF1 expression in the presence of 25 μM cerium ions. Phylogenetic analysis indicated that CQW49_RS02145 was homologous to the Methylorubrum extorquens AM1 lanthanide transporter gene (<i>lutH</i>). These findings suggest that CQW49_RS02145 is involved in lanthanide uptake across the outer membrane. Furthermore, we demonstrated that supplementation with cerium and glycerol caused severe growth arrest in the wild type. CQW49_RS02145 underwent adaptive laboratory evolution in the presence of cerium and glycerol ions, resulting in a mutation that partially mitigated the growth arrest. This finding implies that loss-of-function mutations in CQW49_RS02145 can be attributed to residual glycerol from the frozen stock. <b>IMPORTANCE</b> Lanthanides are widely used in many industrial applications, including catalysts, magnets, and polishing. Recently, lanthanide-dependent metabolism was characterized in methane-utilizing bacteria. Despite the global demand for lanthanides, few studies have investigated the mechanism of lanthanide uptake by these bacteria. In this study, we identify a lanthanide transporter in Methylosinus trichosporium OB3b and indicate the potential interaction between intracellular lanthanide and glycerol. Understanding the genetic and environmental factors affecting lanthanide uptake should not only help improve the use of lanthanides for the bioconversion of methane into valuable products like methanol but also be of value for developing biomining to extract lanthanides under neutral conditions.
Correction to “Turning Toxic Nanomaterials into a Safe and Bioactive Nanocarrier for Co-delivery of DOX/pCRISPR” (2023)

ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionNEXTORIGINAL ARTICLEThis notice is a correctionCorrection to "Turning Toxic Nanomaterials into a Safe and Bioactive Nanocarrier for Co-delivery of DOX/pCRISPR"Navid RabieeNavid RabieeMore by Navid Rabieehttps://orcid.org/0000-0002-6945-8541, Mojtaba Bagherzadeh*Mojtaba BagherzadehMore by Mojtaba Bagherzadehhttps://orcid.org/0000-0003-3009-3693, Amir Mohammad GhadiriAmir Mohammad GhadiriMore by Amir Mohammad Ghadirihttps://orcid.org/0000-0003-4393-5837, Yousef FatahiYousef FatahiMore by Yousef Fatahihttps://orcid.org/0000-0003-0374-2141, Abdullah AldhaherAbdullah AldhaherMore by Abdullah Aldhaher, Pooyan MakvandiPooyan MakvandiMore by Pooyan Makvandi, Rassoul DinarvandRassoul DinarvandMore by Rassoul Dinarvandhttps://orcid.org/0000-0003-0694-7556, Maryam JouyandehMaryam JouyandehMore by Maryam Jouyandeh, Mohammad Reza SaebMohammad Reza SaebMore by Mohammad Reza Saebhttps://orcid.org/0000-0001-7860-5243, Masoud MozafariMasoud MozafariMore by Masoud Mozafarihttps://orcid.org/0000-0002-0232-352X, Mohammadreza Shokouhimehr*Mohammadreza ShokouhimehrMore by Mohammadreza Shokouhimehrhttps://orcid.org/0000-0003-1416-6805, Michael R. Hamblin*Michael R. HamblinMore by Michael R. Hamblin, and Rajender S. Varma*Rajender S. VarmaMore by Rajender S. Varmahttps://orcid.org/0000-0001-9731-6228Cite this: ACS Appl. Bio Mater. 2023, 6, 3, 1313–1314Publication Date (Web):February 14, 2023Publication History Received6 December 2022Published online14 February 2023Published inissue 20 March 2023https://pubs.acs.org/doi/10.1021/acsabm.2c01005https://doi.org/10.1021/acsabm.2c01005correctionACS PublicationsCopyright © 2023 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views497Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (6 MB) Get e-Alertsclose Get e-Alerts
Table S6 from Lentiviral-Driven Discovery of Cancer Drug Resistance Mutations (2023)

&lt;p&gt;Deletions identified by LentiMutate on BCR-ABL cDNA and imatinib in the K562 cell line. Nucleotide position refers to the position within full length BCR-ABL (p210). Values from individual biological replicates are shown.&lt;/p&gt;