gebhart/patrepeval huggingface dataset:
Effects of the bifunctional sulfoxide MMS350, a radiation mitigator, on hematopoiesis in long-term bone marrow cultures and on radioresistance of marrow stromal cell lines. (2015)

The ionizing irradiation mitigator MMS350 prolongs survival of mice treated with total-body irradiation and prevents radiation-induced pulmonary fibrosis when added to drinking water at day 100 after thoracic irradiation. The effects of MMS350 on hematopoiesis in long-term bone marrow culture and on the radiobiology of derived bone marrow stromal cell lines were tested. Long-term bone marrow cultures were established from C57BL/6NTac mice and maintained in a high-humidity incubator, with 7% CO2 and the addition of 100 μM MMS350 at the weekly media change. Over 10 weeks in culture, MMS350 had no significant effect on maintenance of hematopoietic stem cell production, or on nonadherent cells or colony-forming units of hematopoietic progenitor cells. Stromal cell lines derived from non MMS350-treated long-term cultures or control stromal cells treated with MMS350 were radioresistant in the clonogenic survival curve assay. MMS350 is a non-toxic, highly water-soluble radiation mitigator that exhibits radioprotective effects on bone marrow stromal cells.
ChemInform Abstract: Syntheses of Oligosaccharides and Glycopeptides on Insoluble and Soluble Supports (1998)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Compiling for the IBM Matrix Engine for Enterprise Workloads (2022)

The matrix-multiply assist (MMA) facility is the latest addition to IBM’s power instruction set architecture and first shipped in the recently introduced POWER10 processor. MMA is designed to accelerate matrix–matrix operations, such as matrix multiplication and convolution, using instructions that compute the outer product of vector-register operands. Outer product computations have been used for decades in linear algebra libraries to deliver high-performance implementations of matrix operations. Such libraries use conventional single-instruction–multiple-data (SIMD) instructions to emulate outer product operations. MMA in POWER10 is the first hardware with direct support for outer product operations released in the market. MMA operates with the widest diversity of data types compared to any accelerator design currently announced. Unleashing the high-performance enabled by MMA requires careful code generation. Two key considerations for optimal MMA code performance are 1) the choice of accumulation layout when maximizing the using the accumulators and 2) the selection of matrix access order. This article shows that over 92% of peak performance in POWER10 with MMA can be achieved when the code generation makes the right choices.
Accelerating and de‐risking CMC development with transposon‐derived manufacturing cell lines (2021)

The development of highly productive, genetically stable manufacturing cell lines is on the critical path to IND filing for protein-based biologic drugs. Here, we describe the Leap-In Transposase® platform, a novel transposon-based mammalian (e.g., Chinese hamster ovary) cell line development system that produces high-titer stable pools with productivity and product quality attributes that are highly comparable to clones that are subsequently derived therefrom. The productivity distributions of clones are strongly biased toward high producers, and genetic and expression stability is consistently high. By avoiding the poor integration rates, concatemer formation, detrimental transgene recombination, low average expression level, unpredictable product quality, and inconsistent genetic stability characteristic of nonhomologous recombination methods, Leap-In provides several opportunities to de-risk programs early and reduce timelines and resources.
SUPPRESSING MEMORY T CELL ACTIVATION INDUCES ISLET ALLOGRAFT TOLERANCE IN ALLOANTIGEN-PRIMED MICE (2010)

Xia, J.1; Chen, J.2; Shao, W.1; Lan, T.1; Wang, Y.3; Xie, B.1; Thorlacius, H.4; Tian, F.2; Huang, R.5; Qi, Z.6 Author Information
ICI cancellation based channel estimation for OFDM systems (2004)

Orthogonal frequency division multiplexing (OFDM), which is a multicarrier modulation scheme, splits high data rate information symbols into lower rate parallel data streams and transmits this parallel information on different orthogonal carriers. The loss of orthogonality among subcarriers causes inter-carrier interference (ICI), which affects both channel estimation and detection of OFDM data symbols. Channel estimation error degrades the performance of coherent receiver limiting capacity, data rate and performance of the overall OFDM system. This paper proposes a novel frequency-domain channel estimator, which mitigates the effects of ICI by jointly finding the frequency offset and channel frequency response (CFR). Unlike conventional channel estimation techniques, where ICI is treated as part of the noise, the proposed approach considers the effect of frequency offset in estimation of CFR. A binary search algorithm is used to find the present frequency offset and CFR jointly. Variance of the frequency offset estimator and mean-square error (MSE) performances for conventional least-squares (LS) estimator and the proposed method are obtained using computer simulations. The variance of the jointly estimated frequency offset is found to be very close to the Cramer-Rao lower bound. It is shown that the proposed method outperforms LS channel estimator especially in ICI dominated situations.
Fine Force Reproduction of Environmental Haptic Sensations Based on Momentum Control (2016)

This paper focuses on the reproduction system for the environmental haptic sensations. The conventional reproduction system is based on force control. However, the resolution of the force command value is determined by the resolution of the encoder and the D/A board. The resolution of the force command value becomes insufficient in a high-stiffness environment. Because the displacement of the actuator is small, the position information becomes rough. Therefore, a large force vibration is caused by the differential of the momentum of viscosity in the conventional system. Thus, this paper proposes the reproduction system of haptic sensations based on momentum control. The proposed reproduction system indicates that the momentum control is able to change the force command resolution by varying the momentum control gain. Because the resolution of the force command value is changed by the momentum controller, a reproduction of the environmental haptic sensations that have a coarse force resolution is achieved. The effectiveness of the proposed method is confirmed by experiments and analysis.
Dose reconstruction for real‐time patient‐specific dose estimation in CT (2015)

The authors present a method for analytic dose reconstruction in CT, similar to the techniques used in radiation therapy planning with megavoltage energies. Future work will include refinements of the proposed method to improve the accuracy as well as a more extensive validation study. The proposed method is not intended to replace methods that track individual x-ray photons, but the authors expect that it may prove useful in applications where real-time patient-specific dose estimation is required.
Reductions of Cobalt(III) Complexes by Ferrous Chelates. II. The Reductions of Cobalt(III) Complex with Polymeric Ligands by Means of Some Ferrous Chelates (1974)

Abstract The effects of polymeric ligands on the electron-transfer reaction of the cobalt(III) complex have been studied for the reductions of the complex ions of cis-Co(en)2ACl2+ (A=poly-4-vinylpyridine, PVP, or pyridine, py) by the aquo ferrous ion and some Fe(II)-nitrilopolycarboxylate chelates. The specific rate of the Fe2+ reduction of the Co(en)2PVPCl2+ is smaller than that of the Co(en)2PyCl2+. The specific rate of the Fe(II)-chelate reduction increases considerable when the pyridine ligand in the Co(en)2-PyCl2+ is replaced by PVP. The effects of the chelating agents, the degree of coordination, the ionic strength, and the concentration of the reductant on the reduction rate of the Co(en)2ACl2+ by the Fe(II)-nitrilopolycarboxylate were also examined. The resulting reactivity patterns of the polymer complex ions could mainly explained by the effect of the higher electrostatic field caused by the positively-charged polymer structure.
Ion Pair Acidities and Aggregation of Some Amide and Oxazoline Enolates in THF, (1999)

Equilibrium lithium ion pair acidities at 25 °C have been determined in THF for the following acetamides: N,N-dimethyl-(4-biphenylyl)acetamide (1) (19.69), N,N-diethyl-(4-biphenylyl)acetamide (2) (20.30), N,N-dimethyl-diphenylacetamide (3) (20.76), N,N-diethyl-diphenylacetamide (4) (21.99), and 1-(diphenylmethylcarbonyl)pyrrolidine (5) (21.08). These acidity data compare well with those previously reported for the corresponding cesium enolates. Lithium and cesium ion pair acidities in THF are reported for two oxazolines: 2-(4-biphenylylmethyl)oxazoline (6) (Li, 21.53; Cs, 25.49) and (E)-(4S,5R)-2-(4-biphenylylmethyl)-4-methoxymethyl-5-phenyloxazoline (7) (Li, 20.10; Cs 24.21). Studies of their acidities over a wide range of concentrations indicate that all of the lithium and cesium enolates of 1−7 are essentially monomeric at concentrations ranging from 10-3 to 10-4 M. Traces of dimer were detected for the lithium enolates of amides 1 and 2 and for the cesium enolate of oxazoline 6. Their dimerization constants (M-1) were found to be about 450, 200, and 150, respectively. All of the other lithium and cesium enolate probably form small amounts of dimer, but the dimerization constant of <100 M-1 is below the limit of precision of our spectroscopic method.
Supplementary legend from Gene Copy Number Estimation from Targeted Next-Generation Sequencing of Prostate Cancer Biopsies: Analytic Validation and Clinical Qualification (2023)

&lt;p&gt;Supplementary legend&lt;/p&gt;
Middleware, framework and novel computing models for grid and cloud service orchestration (2017)

Highly scalable, resilient commodity hardware along with the availability of huge amounts of data allow consumers and companies to access to a wide range of high-value services through a pay-per-use, pay-as-you-go model. The increasing need for intelligent services at large scale is bringing providers to optimise the use of resources in order to meet the increasing web-scale demand. Programming models are evolving to address the high complexity of service composition, which need resiliency and efficiency. Algorithms and technologies for intelligent orchestration allow multiple owners of cloud and grid infrastructures to satisfy customer requirements and business constraints. We assist in the development of new architectures, frameworks, middlewares and computing models, as well as innovative applications, aimed at supporting scientists and business analysts in the development of unified computing frameworks and data analysis at large scale. This paper summarises a number of contributions given in the area of distributed cloud and grid computing, with particular emphasis on service orchestrations and emergent applications for high-value services.
Regulation of the lysophosphatidylserine and sphingosine 1‐phosphate levels in autologous whole blood by the pre‐storage leukocyte reduction (2016)

LR effectively reduced the accumulation of LysoPS in AB units. On the other hand, it increased concentrations of S1P due to platelet activation by exposure to the filter material. These suggest that increases of S1P levels in LR and LysoPS in the unmodified samples were mainly caused by the leukocytes and/or platelets and that LR was effective in inhibiting the accumulation of LysoPS.
Detection and preliminary characterization of CD8+T lymphocytes specific for Wilms’ tumor antigen in patients with non-Hodgkin lymphoma (2013)

Wilms' tumor antigen (WT1) is overexpressed in many different solid tumors and hematologic malignancies. However, little is known about WT1 expression or WT1-specific immune responses in patients with non-Hodgkin lymphoma (NHL). In a cross-sectional survey study, we investigated the immune recognition of WT1 by patients with NHL. Utilizing a WT1 overlapping peptide library, we discovered that a large percentage of patients with NHL of all grades maintain WT1-specific T cells. Ex vivo frequencies of these T cells measured from unfractionated samples by the CD137 activation marker assay were high in many patients (some > 1% CD8+). Using standard in vitro techniques we discovered that they were cytotoxic to WT1 peptide library-loaded T2 cells and WT1 antigen-primed autologous Epstein-Barr virus-transformed B cell lines (EBV-LCLs) and expressed interferon gamma (IFN-γ). In addition, we detected WT1 mRNA transcripts in diseased lymph node tissues of patients with NHL utilizing real-time quantitative polymerase chain reaction (RT-qPCR) technology. These results are the first example of strong T cell reactivity against WT1 in patients with NHL which also demonstrate strong cytotoxicity against peptide-loaded tumor cells. The potential for developing WT1 as a target for immunotherapy in NHL deserves further exploration.
Identification and characterization of inhibitors of G protein-coupled receptor kinase 6 (GRK6) as potential therapeutics for multiple myeloma (2012)

Multiple myeloma (MM) is one of the most common hematological malignancies, but current therapy options are limited to high-dose chemotherapy or high-risk stem-cell transplantation. In a recent kinome-wide RNAi study by Tiedemann and colleagues (2010), the G-protein coupled receptor kinase 6 (GRK6) was identified as a critical kinase required for survival of MM cells. This study also suggests that MM cells, but not other cell types, are dependent on GRK6; and that gene silencing by shRNA or siRNA of GRK6, but not other GRKs, results in decreased survival. At present, the G protein-coupled receptor (GPCR) signaling mediated by GRK6 in MM cells is not well understood. Through gene silencing techniques and expression of either the wild-type or kinase-dead form of GRK6 protein, we determined that a functional GRK6 kinase domain is required for survival of MM cells. These findings helped validate that the GRK6 kinase domain is a potential target for MM, and have spurred the investigation of small molecule kinase inhibitors of GRK6. By screening a cassette of compounds that are known as kinase inhibitors, compounds with moderate potency in preliminary biochemical assays were identified and further evaluated. As part of this effort we identified CX-4945, which is a known potent and selective ATP-competitive small molecule protein kinase CK2 inhibitor (IC₅₀ of 1 nM for recombinant human CK2α) as a moderately potent inhibitor of GRK6 (IC₅₀ on GRK6 = 300-500 nM). Herein we describe the design and synthesis of novel analogs of CX-4945 and key structure activity relationships (SAR) of this chemical series against GRK6 that serve as a platform for further optimization.
Spiritual Leadership as a Model for Implementing the Baldrige Performance Criteria (2014)

Issues regarding workplace spirituality have received increased attention in the organizational sciences. The implications of workplace spirituality for leadership theory, research, and practice make this a fast growing area of new research and inquiry by scholars. The purpose of this research was to test a dynamic relationship between spiritual leadership and spiritual well-being (i.e., a sense of calling and membership) and key organizational outcomes in a sample of Baldrige recipient organizations. Using structural equation modeling (SEM), results revealed a positive and significant relationship between spiritual leadership and organizational commitment, unit productivity, and life satisfaction. These relationships were explained or mediated by spiritual well-being. Implications for research and practice are discussed.
Force adaptation in human walking with symmetrically applied downward forces on the pelvis (2012)

The application of external constraints and/or applied forces during movement can lead to reactive as well as adaptive changes in human motion. Previous researches in the literature have usually focused on adaptation in human kinematics when external forces were applied using exoskeletons during the swing phase of gait. This work aims to study adaptation in human walking when externally applied forces are present on the pelvis both during the swing and stance phases of the gait. A novel tethered pelvic assist device (TPAD) was used to passively apply downward forces on the human pelvis while walking. During the experiment, healthy subjects walked on a treadmill at a constant speed while their kinematics and foot pressure data were recorded. Data analysis revealed that the healthy subjects exhibited both reactive as well as adaptive changes in their gait parameters. The immediate response of the subjects was to increase their hip flexion to clear their foot off the ground as they were unable to lift their pelvis to their usual height during normal motion. Seven out of eight subjects in the study resisted the downward forces to move their pelvis up. Eventually, they reached a level of downward force that they could sustain over the training. This adaptation to the downward force was reflected in the heel peak pressure values during the cycles of the gait. On removing the tethers, aftereffects in heel peak pressure during the gait cycles were observed.
Biochemical mechanisms of red blood cell 2,3‐diphosphoglycerate increase at high altitude (1980)

Abstract Red blood cell 2,3 diphosphoglycerate (2,3‐DPG) levels increase after ascent to high altitude. Studies were undertaken to identify the biochemical mechanisms responsible for eliciting the 2,3‐DPG response in several types of subjects. These included (1) short‐term exposure to 3400 m in ten subjects; (2) exposure to 4300 m in an additional ten subjects; (3) studies in 28 high‐altitude normal residents of 3100 m; and (4) studies in 28 high‐altitude residents with chronic mountain polycythemia. Controls were 41 residents of 240 m. Regression analysis identified the glycolytic variables, termed “key variables,” on which variation in 2,3‐DPG levels was dependent (P &lt; .05). Key variables common to the short‐term studies were glucose‐6‐phosphate, phosphoenolpyruvate, and the ratio of the levels of adenosine diphosphate to adenosine triphosphate. The positions of these key variables in the glycolytic pathway and their mean levels suggest erythrocyte hexokinase and pyruvate kinase activation as possible enzymatic mechanisms. Key variables unique to the 3400 m study suggested phosphofructokinase activation also acted to increase 2,3‐DPG levels. 2,3‐DPG levels in the normal 3100 m residents were not different from low‐altitude values, and 2,3‐DPG levels in these samples did not appear to be dependent on any of the glycolytic variables examined. Among the high‐altitude residents with polycythemia, higher 2,3‐DPG levels were dependent on glucose‐6‐phosphate, fructose diphosphate, dihydroxyacetone phosphate, and the ratio of adenosine diphosphate to adenosine triphosphate levels. The positions of these variables in the glycolytic pathway and their mean levels suggested activation of the hexokinase and phosphofructokinase enzymes.
Adaptive optimization of high harmonic generation (2000)

Summary form only given. We demonstrate that the intensity and spectral characteristics of high harmonics generated by an ultrafast laser can be optimized using shaped pulses to drive the process. Using an evolutionary strategy algorithm and a deformable mirror pulse shaper, the generation of a particular harmonic was enhanced by a factor of 4 beyond what can be obtained using a transform-limited driving pulse. We also demonstrate that a particular wavelength region can be spectrally selected by enhancing certain harmonics while suppressing adjacent harmonics. This ability is very important for certain application experiments, because other methods to spectrally select a particular harmonic can distort the ultrafast time structure of the harmonics. These data are preliminary because they were obtained using 30-fs laser pulses. We expect significant improvement in future by using 5 to 15 fs shaped laser pulses.
Pitfalls in genetic testing: the story of missed SCN1A mutations (2016)

We illustrate the pitfalls of Sanger sequencing and most importantly provide evidence that SCN1A mutations are an even more frequent cause of DS than already anticipated.
Forward problem solution of EMSIs using BEM with isoparametric cubic elements (2003)

Numerical solution of the potential and magnetic fields for a given electrical source distribution in the human brain is an essential part of the electro-magnetic source imaging. In this study, a Boundary Element Method (BEM) is used that employs 10-noded cubic triangular elements. The results are given in comparison with the solutions obtained using linear and quadratic elements. The relative difference measure obtained for the numerical models that use different element types show the superiority of quadratic and cubic element types.
Establishment of macaque trophoblast stem cell lines derived from cynomolgus monkey blastocysts (2020)

The placenta forms a maternal-fetal junction that supports many physiological functions such as the supply of nutrition and exchange of gases and wastes. Establishing an in vitro culture model of human and non-human primate trophoblast stem/progenitor cells is important for investigating the process of early placental development and trophoblast differentiation. In this study, we have established five trophoblast stem cell (TSC) lines from cynomolgus monkey blastocysts, named macTSC #1-5. Fibroblast growth factor 4 (FGF4) enhanced proliferation of macTSCs, while other exogenous factors were not required to maintain their undifferentiated state. macTSCs showed a trophoblastic gene expression profile and trophoblast-like DNA methylation status and also exhibited differentiation capacity towards invasive trophoblast cells and multinucleated syncytia. In a xenogeneic chimera assay, these stem cells contributed to trophectoderm (TE) development in the chimeric blastocysts. macTSC are the first primate trophoblast cell lines whose proliferation is promoted by FGF4. These cell lines provide a valuable in vitro culture model to analyze the similarities and differences in placental development between human and non-human primates.
On the design of cooperative road infrastructure systems (2008)

This paper discusses the design of cooperative road infrastructure systems for infrastructure-based driving support functions. The background of such systems is mapped out and it is shown that there is a need for a cross disciplinary approach. Using an example of a support function, namely the overtaking support, it is shown that such a system is feasible. The different challenges and technological problems that are identified are given and the future work is indicated.
Partially Linear Bayesian Estimation with Application to Sparse Approximations (2011)

We address the problem of estimating a random vector X from two sets of measurements Y and Z, such that the estimator is linear in Y. We show that the partially linear minimum mean squared error (PLMMSE) estimator does not require knowing the joint distribution of X and Y in full, but rather only its second-order moments. This renders it of potential interest in various applications. We further show that the PLMMSE method is minimax-optimal among all estimators that solely depend on the second-order statistics of X and Y. Finally, we demonstrate our approach in the context of recovering a vector, which is sparse in a unitary dictionary, from two sets of noisy measurements. We show that in this setting PLMMSE estimation has a clear computational advantage, while its performance is comparable to state-of-the-art algorithms.