gebhart/patrepeval huggingface dataset:
Platelet lysates-based hydrogels incorporating bioactive mesoporous silica nanoparticles for stem cell osteogenic differentiation (2021)

Scaffolds for bone tissue regeneration should provide the right cues for stem cell adhesion and proliferation, but also lead to their osteogenic differentiation. Hydrogels of modified platelet lysates (PLMA) show the proper mechanical stability for cell encapsulation and contain essential bioactive molecules required for cell maintenance. We prepared a novel PLMA-based nanocomposite for bone repair and regeneration capable of releasing biofactors to induce osteogenic differentiation. Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) were encapsulated in PLMA hydrogels containing bioactive mesoporous silica nanoparticles previously loaded with dexamethasone and functionalized with calcium and phosphate ions. After 21 d of culture, hBM-MSCs remained viable, presented a stretched morphology, and showed signs of osteogenic differentiation, namely the presence of significant amounts of alkaline phosphatase, bone morphogenic protein-2 and osteopontin, hydroxyapatite, and calcium nodules. Developed for the first time, PLMA/MSNCaPDex nanocomposites were able to guide the differentiation of hBM-MSCs without any other osteogenic supplementation.
Performance comparison of streak camera recording systems (1995)

Streak camera based diagnostics are vital to the inertial confinement fusion program at Sandia National Laboratories. Performance characteristics of various readout systems coupled to an EGG-AVO streak camera were analyzed and compared to scaling estimates. The purpose of the work was to determine the limits of the streak camera performance and the optimal fielding conditions for the Amador Valley Operations (AVO) streak camera systems. The authors measured streak camera limitations in spatial resolution and sensitivity. Streak camera limits on spatial resolution are greater than 18 lp/mm at 4% contrast. However, it will be difficult to make use of any resolution greater than this because of high spatial frequency variation in the photocathode sensitivity. They have measured a signal to noise of 3,000 with 0.3 mW/cm{sup 2} of 830 nm light at a 10 ns/mm sweep speed. They have compared lens coupling systems with and without micro-channel plate intensifiers and systems using film or charge coupled device (CCD) readout. There were no conditions where film was found to be an improvement over the CCD readout. Systems utilizing a CCD readout without an intensifier have comparable resolution, for these source sizes and at a nominal cost in signal to noise of 3, over those with an intensifier. Estimates of the signal-to-noise for different light coupling methods show how performance can be improved.
An assessment of Motorola CodeLinkTM microarray performance for gene expression profiling applications (2002)

DNA microarrays enable users to obtain information on differences in transcript abundance on a massively parallel scale. Recently, however, data analyses have revealed potential pitfalls related to image acquisition, variability and misclassifications in replicate measurements, cross-hybridization and sensitivity limitations. We have generated a series of analytical tools to address the manufacturing, detection and data analysis components of a microarray experiment. Together, we have used these tools to optimize performance in an expression profiling study. We demonstrate three significant advantages of the Motorola CodeLink platform: sensitivity of one copy per cell, coefficients of variation of 10% in the hybridization signals across slides and across target preparations, and specificity in distinguishing highly homologous sequences. Slides where oligonucleotide probes are spotted in 6-fold redundancy were used to demonstrate the effect of replication on data quality. Lastly, the differential expression ratios obtained with the CodeLink expression platform were validated against those obtained with quantitative reverse transcription-PCR assays for 54 genes.
MP08-17 IS MAGNETIC RESONANCE IMAGING SENSITIVE ENOUGH FOR PARTIAL GLAND HIGH INTENSITY FOCUSED ULTRASOUND TREATMENT? COMPARING PROSTATE CANCER LESIONS BETWEEN MAGNETIC RESONANCE IMAGING AND PROSTATECTOMY SPECIMENS (2017)

imaging was observed in 68 patients, 7.4% of the patients with any axial imaging, 3.6% of the overall cohort. Patients with duplicate imaging differed significantly from the remainder of the cohort in clinicopathologic characteristics (higher PSA, p<0.001; higher cT stage, p0.015; higher Grade Group, p<0.001; higher NCCN risk category, p<0.001) but not demographic characteristics (age, race, family history of CaP, Charlson comorbidity score). 48% of providers were observed to utilize duplicate axial imaging, with significant variation by individual provider from 0% to 60%. A weak correlation was observed between individual provider 0 s patient volume and use of duplicate imaging (Spearman 0 s correlation 0.313, n56, p0.019).
Author Correction: LepVax, a defined subunit vaccine that provides effective pre-exposure and post-exposure prophylaxis of M. leprae infection (2018)

[This corrects the article DOI: 10.1038/s41541-018-0050-z.].
Adaptive pre-distortion techniques based on orthogonal polynomials (2010)

Pre-distortion in digital baseband is a cost-effective method to linearise the transmit power amplifiers of spectrally efficient communication systems. From a signal processing point of view, the functional structure of the pre-distorter is primarily determined by the decision which model to choose, as well as by the selected adaptive algorithm. During the last two decades, in literature, a multitude of pre-distorter structures has been proposed and analysed. In this work, we focus on simple and commonly employed models (the Wiener model and the Hammerstein model) consisting of a linear filter and a static nonlinearity. The latter is represented using a basis of orthogonal polynomials. First, applying practical transmission signals, different orthogonal polynomial bases are compared with respect to the numerical condition of least squares estimation, and with respect to the convergence behaviour of gradient methods. In a second step, pre-distorters which employ orthogonal polynomials are adapted by the indirect learning structure, respectively, the nonlinear filtered-x least mean squares algorithm. Based on simulations and burst measurements with a commercial power amplifier, the real-world performance of such pre-distortion systems is investigated.
Development of pollinium in two epidendroid orchids (2006)

Development of pollinium has been studied in Cymbidium aloifolium (L.) Sw. (tribe Cymbidieae) and Smitinandia micrantha (Lindl.) Holtt. (tribe Vandeae) of the family Orchidaceae. The anther primordium develops two lobes oriented towards the labellum. Each lobe has a group of archesporial cells, which divide mitotically so as to form a spherical mass of sporogenous cells, in which there is differentiation of a dorsiventral plate of elongated sterile cells. In C. aloifolium, the sterile plate extends into about three-fourths of the fertile tissue, whereas in S. micrantha it is complete but asymmetrically oriented. Synchronous meiosis in the microsporocytes is followed by simultaneous cytokinesis so as to form microspore tetrads which organize into pollinia. Centripetal disintegration of the sterile plate during development of the male gametophyte forms two perforate (hollow) pollinia in C. aloifolium, but four unequal pollinia in S. micrantha. The anther wall is composed of an epidermis, an endothecium, two to three middle layers and the tapetum. The tapetum disorganizes during development of the male gametophyte, while the endothecium and adjacent middle layers develop fibrous thickenings.
Prophylactic Activity of Orally Administered FliD-Reactive Monoclonal SIgA Against Campylobacter Infection (2020)

<i>Campylobacter</i> infection is one of the most common causes of bacterial gastroenteritis worldwide and a major global health threat due to the rapid development of antibiotic resistance. Currently, there are no vaccines approved to prevent campylobacteriosis, and rehydration is the main form of therapy. Secretory immunoglobulin A (SIgA) is the main antibody class found in mucous secretions, including human milk, and serves as the first line of defense for the gastrointestinal epithelium against enteric pathogens. In this study, we describe the prophylactic activity of orally delivered recombinant SIgA generated from two human monoclonal antibodies (CAA1 and CCG4) isolated for their reactivity against the flagellar-capping protein FliD, which is essential for bacteria motility and highly conserved across <i>Campylobacter</i> species associated with severe enteritis. In an immunocompetent weaned mouse model, a single oral administration of FliD-reactive SIgA CAA1 or CCG4 at 2 h before infection significantly enhances <i>Campylobacter</i> clearance at early stages post-infection, reducing the levels of inflammation markers associated with epithelial damage and polymorphonuclear (PMN) cells infiltration in the cecum lamina propria. Our data indicate that the prophylactic activity of CAA1 and CCG4 is not only dependent on the specificity to FliD but also on the use of the SIgA format, as the immunoglobulin G (IgG) versions of the same antibodies did not confer a comparable protective effect. Our work emphasizes the potential of FliD as a target for the development of vaccines and supports the concept that orally administered FliD-reactive SIgA can be developed to prevent or mitigate the severity of <i>Campylobacter</i> infections as well as the development of post-infection syndromes.
Supplementary Table 1 from Inhibition of TGF-β Enhances the <i>In Vivo</i> Antitumor Efficacy of EGF Receptor–Targeted Therapy (2023)

&lt;p&gt;PDF file - 256K, Clinical characteristics of patients with HNSCC&lt;/p&gt;
Modelling elastic media with the wavelet transform (2001)

We present a new method for modelling 2-D elastic media with the application of the wavelet transform, which is also extended to cases where discontinuities simulate geological faults between two different elastic media. The basic method consists of the discretization of the polynomial expansion for the boundary conditions of the 2-D problem involving the stress and strain relations for the media. This parametrization leads to a system of linear equations that should be solved for the determination of the expansion coefficients, which are the model parameters, and their determination leads to the solution of the problem. The wavelet transform is applied with two main objectives, namely to decrease the error related to the truncation of the polynomial expansion and to make the system of linear equations more compact for computation. This is possible due to the properties of this finite length transform. The method proposed here was tested for six different cases for which the analytical solutions are known. In all tests considered, we obtained very good matches with the corresponding known analytical solutions, which validate the theoretical and computational parts of the project. We hope that the new method is useful for modelling real media.
PEM/AEM Junction Design for Bipolar Membrane Fuel Cells (2017)

The combination of proton exchange membrane (PEM) and anion exchange membrane (AEM) materials to form a bipolar membrane (BPM) is of interest in hybrid electrochemical devices to mitigate the disadvantages of their monopolar counterparts. The PEM-AEM interface is a critical component in bipolar membrane fuel cell operation. In this study, mono- and di-membrane bipolar membranes were fabricated. Interfacial materials with varying conductivities were used in order to control the location of the junction within the di-membrane BPMs. Mono-membrane BPMs were constructed via conversion of a single face of a monopolar membrane (Nafion). The membranes were used in fully functional fuel cells and characterized via electrochemical impedance spectroscopy (EIS). For the di-membrane BPMs, use of a conductive interface consisting of a single ion conductive material resulted in devices with lower interfacial resistance as compared to a neutral interface. When comparing conductive interface materials, anion-conductive materials provided lower total membrane resistance than proton-conductive materials. This decrease is due to positioning the junction closer to the anode and farther from the air-cathode. These results show that the formation of the optimal junction is critically dependent on fabrication technique and location.
North-centred orientation tracking on mobile phones (2010)

Magnetic compasses and accelerometers provide absolute orientation measurements within the earth's reference frame. However, sensor output typically suffers from jitter and external disturbances. Conversely, visual tracking provides more stable orientation estimation relative to an unknown initial orientation rather than to true north. We propose a 3-degree-of-freedom orientation tracking approach combining the accuracy and stability of vision tracking with the absolute orientation from inertial and magnetic sensors by estimating the offset between the initial orientation of the vision tracker and true north. We demonstrate that the approach improves absolute orientation estimation on a mobile phone device.
A first‐in‐human pharmacodynamic and pharmacokinetic study of a fully human anti‐glucagon receptor monoclonal antibody in normal healthy volunteers (2017)

REGN1193, a GCGR-blocking monoclonal antibody, produced a safety, tolerability and PK/PD profile suitable for further clinical development. The occurrence of transient elevations in serum hepatic aminotransferases observed here and reported with several small molecule glucagon receptor antagonists suggests an on-target effect of glucagon receptor blockade. The underlying mechanism is unknown.
Adoptive Infusion of Allogeneic KIR Ligand Mismatched NK Cells Reduce GVHD in an MHC Matched Allogeneic Stem Cell Transplantation Model. (2005)

Abstract Retrospective data suggest NK cells play a role in protecting recipients from graft versus host disease (GVHD) in the setting of killer IgG-like receptor (KIR) ligand incompatibility. In humans, this protective effect is most evident with MHC mismatched transplantation, usually following in vivo or in vitro T-cell depletion. In MHC mismatched murine transplant models, lethal GVHD is reduced following the adoptive infusion of KIR ligand mismatched NK cells; it is unknown whether NK cells can mediate similar protective effects following MHC matched transplantation. Therefore, we investigated the impact of adoptively infusing KIR ligand mismatched NK cells on GVHD in an MHC matched T-cell replete murine model of allogeneic transplantation. Balb/C recipient mice underwent allogeneic bone marrow (8 x 106 cells) and splenocyte (15 x 106 cells) transplantation from B10.d2 donors following 950cGy of irradiation. Allogeneic B10.d2 donor NK cells were first isolated by negative depletion using magnetic beads selecting for CD4, CD5, CD8a, CD19, Gr-1 and Ter-119, and then expanded over 4-6 days in vitro in DMEM media containing 10% FCS and 500U/ml of IL-2. NK cell subsets (KIR ligand matched vs. KIR ligand mismatched) were then isolated by flow cytometry into Ly49I/C+ NK cells (KIR ligand mismatched in the GVHD direction for Balb/C recipients) and Ly49A/G+ NK cells (KIR ligand matched for Balb/C recipients). On day +4, recipient mice received a single tail vein injection with either KIR ligand matched, KIR ligand mismatched or unsorted “bulk” NK cells (0.5–1.0 x 106 NK cells). All (9/9) control transplant recipients (no adoptive NK cell infusion) as well as recipients of Ly49A/G (KIR ligand matched) NK cells (13/13) developed skin GVHD, in contrast to 4/7 (57%, p=0.03) recipients of bulk NK cells and only a minority (13% [1/8], p &amp;lt; 0.01) of animals receiving KIR ligand mismatched NK cells. Using a cumulative clinical GVHD scoring system (total score = 9), overall GVHD was decreased in recipients of KIR ligand mismatched NK cells (median score = 0 at day +45) compared to mice that received KIR ligand matched NK cells (median score = 3; p = 0.15) or no NK cells (median score = 3; p= 0.12); no significant difference in survival was observed between cohorts. This murine model provides the first in vivo evidence that adoptively infused KIR ligand mismatched allogeneic NK cells reduce GVHD following T-cell replete MHC matched allogeneic transplantation. The impact of infusing multiple doses of KIR ligand mismatched NK cells on GVHD and their ability to induce a graft-vs-tumor effect in tumor bearing Balb/c mice is currently being evaluated.
EFSUMB Guidelines and Recommendations on the Clinical Use of Ultrasound Elastography. Part 1: Basic Principles and Technology (2013)

The technical part of these Guidelines and Recommendations, produced under the auspices of EFSUMB, provides an introduction to the physical principles and technology on which all forms of current commercially available ultrasound elastography are based. A difference in shear modulus is the common underlying physical mechanism that provides tissue contrast in all elastograms. The relationship between the alternative technologies is considered in terms of the method used to take advantage of this. The practical advantages and disadvantages associated with each of the techniques are described, and guidance is provided on optimisation of scanning technique, image display, image interpretation and some of the known image artefacts.
Does Lift-Off Occur in Unconstrained Non-Conforming Total Knee Arthroplasty? (1998)

Abstract Despite its widespread use in the orthopedic community, ultra-high molecular weight polyethylene (UHMWPE) remains the weak link in the prosthesis material chain. The vast majority of wear debris generated in total joint replacements originates from the UHMWPE component. These wear products lead to osteolysis, implant loosening and the eventual need for revision surgery. Therefore, the wear of UHMWPE is currently a significant concern in the orthopedic and material science communities. The current study focuses on the dimensional change wear in two types of unconstrained and non-conforming Miller-Galante retrieved tibial components. The hypothesis tested was: lift-off of the lateral side of the femoral component, which would produce large dimensional change near the outer medial compartment edges of the tibial components, does not occur in non-conforming tibial inserts.
Supplemental Table S1 from Histone Deacetylase HDAC8 Promotes Insulin Resistance and β-Catenin Activation in NAFLD-Associated Hepatocellular Carcinoma (2023)

&lt;p&gt;Targeted gene expression profiling of 115 chromatin regulators in murine obesity-promoted HCC models.&lt;/p&gt;
The polyoxo-22-palladate(ii), [Na2PdII22O12(AsVO4)15(AsVO3OH)]25− (2016)

The polyoxo-22-palladate [Na2PdO12(As(V)O4)15(As(V)O3OH)](25-) (1), which represents a novel polyoxo-noble-metalate structural type, was synthesized by reaction of Pd(2+) and AsO4(3-) ions in aqueous solution. Polyanion 1 comprises two {NaPd11} units linked by two arsenate bridges, and hence represents the first example of a defect, monolacunary {Pd11} polyoxopalladate nanocube with arsenate capping groups. The title polyanion was characterized in the solid state as well as by theoretical calculations.
Abstract 4050: Quantitation of sirtuin activity with a one-step luminescent assay (2011)

Abstract The Sirtuin family of histone/protein deacetylating enzymes has gained considerable interest both for their recognized importance in processes such as gene silencing and expression and in cellular metabolism. This has led to identification of these enzymes as important actors in diverse pathologies including diabetes, Alzheimer's disease and oncology. We have developed a convenient and sensitive screening methodology to detect and measure both inhibitors and activators of sirtuin activity. Here we describe a one-step, homogeneous luminescent assay for the detection of activity for multiple sirtuin isotypes. The reagent formulation contains a pro-luminescent sirtuin peptide substrate, NAD+, ATP, thermostable luciferase, and a developer enzyme that cleaves deacetylated lysine residues. The addition of an active sirtuin enzyme source leads to a robust luminescent signal within minutes with “glow-type” characteristics lasting for several hours. Data will be presented showing characterization of the assay using a diverse assortment of established sirtuin inhibitors, followed by suitability for screening in 384-well formats and the incorporation of a control substrate to identify false positives. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 4050. doi:10.1158/1538-7445.AM2011-4050
Depth Transfer: Depth Extraction from Video Using Non-Parametric Sampling (2014)

We describe a technique that automatically generates plausible depth maps from videos using non-parametric depth sampling. We demonstrate our technique in cases where past methods fail (non-translating cameras and dynamic scenes). Our technique is applicable to single images as well as videos. For videos, we use local motion cues to improve the inferred depth maps, while optical flow is used to ensure temporal depth consistency. For training and evaluation, we use a Kinect-based system to collect a large data set containing stereoscopic videos with known depths. We show that our depth estimation technique outperforms the state-of-the-art on benchmark databases. Our technique can be used to automatically convert a monoscopic video into stereo for 3D visualization, and we demonstrate this through a variety of visually pleasing results for indoor and outdoor scenes, including results from the feature film Charade.
Supplementary Table 1 from Epithelial–Mesenchymal Transitioned Circulating Tumor Cells Capture for Detecting Tumor Progression (2023)

&lt;p&gt;Supplementary Table 1. Clinical Parameters in patients&lt;/p&gt;