gebhart/patrepeval huggingface dataset:
Comparison of Carrot Broth- and Selective Todd-Hewitt Broth-Enhanced PCR Protocols for Real-Time Detection of Streptococcus agalactiae in Prenatal Vaginal/Anorectal Specimens (2008)

The reporting of accurate Streptococcus agalactiae screening results in a short time frame is of tremendous clinical benefit. A total of 203 consecutive primary vaginal/anorectal specimens were cultured in selective Todd-Hewitt broth (LIM broth) and with the StrepB carrot broth kit (carrot broth). One-day broth cultures were subjected to both centrifugation and clarification of a 500-mul aliquot prior to sample lysis (protocol A) and direct lysis of a 50-mul aliquot (protocol B). The lysates were subsequently analyzed by the BD GeneOhm StrepB assay. The results were compared to the carrot broth culture results derived from visualization of pigment on day 1 or from a subculture of carrot broth. Thirty-four carrot broth cultures (16.7%) generated diagnostic pigment following overnight incubation; an additional 26 (12.8%) were positive for S. agalactiae upon subculture. Carrot broth-enhanced PCR by the use of either protocol A or protocol B trended toward a higher rate of positive results (33.0%) than the rate observed by either the LIM broth-enhanced PCR (30.5%) or full carrot broth culture analysis (29.6%). In the context of the result on day 1, both carrot broth- and LIM broth-enhanced PCRs generated more true-positive results (P < 0.001) than carrot broth culture visualization. The predictive values for both protocols of carrot broth- or LIM broth-enhanced PCR were >/=95.4%. Whereas protocol A resolved the results for 99.8% of the specimens in the evaluation upon initial testing, a 5.7% initial unresolved rate and a 1.5% final unresolved rate were determined by the use of protocol B. The use of carrot broth within a rapid and highly accurate molecular reflex testing algorithm can limit follow-up testing to cultures without evidence of pigmentation.
Reduction of Ionic Conduction Loss in Multilayer Dielectric Films by Immobilizing Impurity Ions in High Glass Transition Temperature Polymer Layers (2018)

Current development of advanced power electronics for electric vehicles demands high temperature, high energy density, and low loss polymer dielectrics. Multilayer films (MLFs), which are comprised of alternating high temperature/low loss linear dielectric polymer such as polysulfone (PSF) and high energy density polymer such as poly(vinylidene fluoride) (PVDF), are promising for this application, because high temperature tolerance, high energy density, and low loss can be achieved simultaneously. This study explored the reduction of impurity ion conduction loss in PSF/PVDF MLFs (e.g., the dissipation factor is as low as 0.003 at 1 Hz and 100 °C) without sacrificing high dielectric constant and high energy density. Various electric poling processes were explored at a temperature slightly below the glass transition temperature (Tg ∼ 185 °C) of PSF. Compared with pure alternating current (AC) and pure direct current (DC) poling methods, unipolar (DC + AC) poling was found to be the most effective in polarizing impurity ions from the PVDF layers into the PSF layers. Because of the low segmental mobility below Tg, impurity ions were largely “locked” in PSF. The immobilization of impurity ions was thermally stable up to 120 °C. Because DC-link capacitors work with unipolar charge and discharge processes, these PSF/PVDF MLFs with low dielectric losses are promising for the application of advanced power electronics for the automobile industry.
System level trade-offs of microprocessor supply voltage reduction (2010)

This paper examines the impact of decreasing microprocessor supply voltage on system efficiency and performance. While digital designers can decrease power consumption in a microprocessor by lowering its supply voltage, efficiency and performance trade-offs arise in the power supply that offset the power reduction. Conduction losses and switching losses in a buck converter are the primary losses in a switching voltage regulator, and they tend to increase with decreased output voltage. A lower supply voltage can cause control challenges, introduce sensitivity limitations, and reduce overall system efficiency. An efficiency and performance analysis is presented, based on a 12 V board-level power source employed with a low-output-voltage buck converter. The analysis demonstrates system-level limitations of supply voltage reduction.
Predictors of survival and ability to wean from short-term mechanical circulatory support device following acute myocardial infarction complicated by cardiogenic shock (2017)

Among AMI-CS patients requiring short-term MCSDs, age and cardiac index predict survival to discharge. Angiographic result and cardiac index predict ventricular recovery but 50% of those optimally revascularized still required heart replacement therapy.
57th Denver X-ray Conference and selected papers for the special June issue (2009)

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A MUC1/IL-18 DNA vaccine induces protective immunity against subcutaneous MUC1+ tumors in MUC1 transgenic mice (2004)

Proc Amer Assoc Cancer Res, Volume 45, 2004

4845 

MUC1 (mucin 1) is a transmembrane glycoprotein that is normally expressed on the ductal epithelia of pancreas, breast, prostate, colon, and lung. However, in adenocarcinomas of these organs, this self-antigen is over-expressed and aberrantly glycosylated. Thus, a therapy that targets tumor cells expressing this form of MUC1 could have great value in the treatment of these diseases. For the evaluation of MUC1 vaccine candidates, a human MUC1 transgenic mouse (huMUC1 Tg) model was used. These mice express human MUC1 in the appropriate tissue-specific pattern, thereby making MUC1 a self-antigen. Two MUC1+ tumor models were used to test MUC1 vaccine candidates, a subcutaneous tumor model and a lung metastasis model. Using the subcutaneous tumor model, we found that intradermal vaccination with a DNA vaccine encoding MUC1 (pMUC1) alone was insufficient to break tolerance to MUC1 or to induce tumor protection in a subcutaneous tumor model. However, co-administration of pMUC1 with an adjuvant plasmid encoding interleukin-18 (pIL-18) resulted in significant protection from tumor development. This protection was durable in the absence of additional vaccination as demonstrated by protection of vaccinated mice from a second challenge with MUC1+ tumors. Evidence of epitope spreading was also observed when protected mice survived a third tumor challenge in which MUC1- tumor cells were administered. Antibody-mediated depletion of specific lymphocyte subsets suggests that the mechanism of protection is due to the induction of a cellular immune response against MUC1. These results demonstrate that vaccination with pMUC1/pIL-18 can break tolerance to MUC1 and can induce an immune response that mediates decreased tumor burden and increased survival. Furthermore, by mediating tumor regression of MUC1+ tumors, the cellular immune response induced by vaccination with pMUC1/pIL-18 ‘spread’ to other tumor-associated determinants (epitope spreading) leading to the regression of MUC1- tumors. Therefore, it is likely that DNA vaccination could provide clinical benefit in patients with MUC1+ tumors.
Dissociation between the state of leukocyte adhesiveness/aggregation in the peripheral blood and the availability of the CD11B/CD18 and CD62L antigens on the surface of the cells in patients with stress. (1998)

We adopted whole blood flow cytometry and direct labeling of the CD11b/CD18 and the CD62L antigens to study the relationship between their expression on the surface of peripheral leukocytes and the state of leukocyte adhesiveness/aggregation (LAA) as revealed by the leukergy test. We examined patients with infection/inflammation, acute stress and controls. The mean +/- S.D. channel fluorescence intensity of CD11b/CD18 antigen did not differ between patients with infection/inflammation (173 +/- 78) and controls (167 +/- 72). However, a significant (p < 0.0001) difference between these groups was noted regarding LAA state. There was a significant (p = 0.04) reduction in CD11b/CD18 in stress (135 +/- 60) and a significant (p < 0.001) increment in LAA. In both study groups, there was a significant reduction in CD62L. Patients were divided into those with CD11b/CD18 above and below the control's average. No correlation was found between the antigens and LAA. We assume that LAA in patients with stress state is CD11b/CD18 and CD62L independent.
Force-Mediated Endocytosis of Iron Oxide Nanoparticles for Magnetic Targeting of Stem Cells (2023)

Stem cell therapy represents one of the most promising approaches for tissue repair and regeneration. However, the full potential of stem cell therapy remains to be realized. One major challenge is the insufficient homing and retention of stem cells at the desired sites after <i>in vivo</i> delivery. Here, we provide a proof-of-principle demonstration of magnetic targeting and retention of human muscle-derived stem cells (hMDSCs) <i>in vitro</i> through magnetic force-mediated internalization of magnetic iron oxide nanoparticles (MIONs) and the use of a micropatterned magnet. We found that the magnetic force-mediated cellular uptake of MIONs occurs through an endocytic pathway, and the MIONs were exclusively localized in the lysosomes. The intracellular MIONs had no detrimental effect on the proliferation of hMDSCs or their multilineage differentiation, and no MIONs were translocated to other cells in a coculture system. Using hMDSCs and three other cell types including human umbilical vein endothelial cells (HUVECs), human dermal fibroblasts (HDFs), and HeLa cells, we further discovered that the magnetic force-mediated MION uptake increased with MION size and decreased with cell membrane tension. We found that the cellular uptake rate was initially increased with MION concentration in solution and approached saturation. These findings provide important insight and guidance for magnetic targeting of stem cells in therapeutic applications.
Thermo-optical modeling of high power operation of 2 μm codoped Tm,Ho solid-state lasers (2008)

The results of coupled thermo-optical modeling of Tm,Ho solid-state laser operation are reported. A rate dynamics model integrated together with a TEM00 distribution for the total number of stimulated photons is coupled with a two-dimensional time-dependent heat transfer model. The heat transfer model includes absorption, heat release, and transfer inside the crystal as well as the thermal effect of the spontaneously emitted infrared radiation. In water cooled crystal operation this radiation is shown to be absorbed within the water boundary layer, producing significant inhibition of dissipation of the heat released inside the crystal. This effect leads to crystal superheating and significant inhibition of laser energy output. Absorption loss, in particular, due to water vapor present in the cavity is found to decrease significantly laser pulse energy. Numerical results are compared to previous (Tm,Ho:yttrium lithium fluoride) and new (Tm,Ho:ceramic yttrium aluminum garnet) experimental data and discussed within the framework of the computational model and simplified analytical approximations.
ChemInform Abstract: Solid Supported Reactions and Reagents. Part 15. Condensation of . alpha.‐Cyanothioacetamide with Aldehydes Catalyzed by Envirocat EPZG. (1997)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Characterization of a cyclic nucleotide- and calcium-independent neurofilament protein kinase activity in axoplasm from the squid giant axon. (1986)

The phosphorylation activity associated with a neurofilament-enriched cytoskeletal preparation isolated from the squid giant axon has been studied and compared to the phosphorylation activities in intact squid axoplasm. The high molecular weight (greater than 300 kDa) and 220-kDa neurofilament proteins are the major endogenous substrates for the kinases in the axoplasm and the neurofilament preparation, whereas 95- and less than 60-kDa proteins are the major phosphoproteins in the ganglion cell preparation. The squid axon neurofilament (SANF) protein kinase activity appeared to be both cAMP and Ca2+ independent and could phosphorylate both casein (Km = 40 microM) and histone (Km = 180 microM). The SANF protein kinase could utilize either ATP or GTP in the phosphotransferase reaction, with a Km for ATP of 58 microM and 129.4 microM for GTP when casein was used as the exogenous substrate; and 25 and 98.1 microM for ATP and GTP, respectively, when the endogenous neurofilament proteins were used as substrates. The SANF protein kinase activity was only slightly inhibited by 2,3-diphosphoglycerate and various polyamines at high concentrations and was poorly inhibited by heparin (34% inhibition at 100 micrograms/ml). The failures of heparin to significantly inhibit and the polyamines to stimulate the SANF protein kinase indicate that it is not a casein type II kinase. The relative efficacy of GTP as a phosphate donor indicates that SANF protein kinase differs from known casein type I kinases. Phosphorylated (32P-labeled) neurofilament proteins were only slightly dephosphorylated in the presence of axoplasm or stellate ganglion cell supernatants, and the neurofilament-enriched preparation did not dephosphorylate 32P-labeled neurofilament proteins. The axoplasm and neurofilament preparations had no detectable protein kinase inhibitor activity, but a strong inhibitor activity, which was not dialyzable but was heat inactivatable, was found in ganglion cells. This inhibitor activity may account for the low phosphorylation activity found in the stellate ganglion cells and may indicate inhibitory regulation of SANF protein kinase activity in the ganglion cell bodies.
Measurement of the isolated diphoton cross section inppcollisions ats=7  TeVwith the ATLAS detector (2012)

The ATLAS experiment has measured the production cross section of events with two isolated photons in the final state, in proton-proton collisions at ffiffi ffi s p 7 TeV. The full data set acquired in 2010 is used, corresponding to an integrated luminosity of 37 pb 1 . The background, consisting of hadronic jets and isolated electrons, is estimated with fully data-driven techniques and subtracted. The differential cross sections, as functions of the di-photon mass (m ), total transverse momentum (p T; ), and azimuthal separation ( ), are presented and compared to the predictions of next-toleading-order QCD.
Electromagnetic analysis of UHF near-field RFID tag antenna (2011)

In this paper, the performance of Near-Field UHF RFID systems is investigated by means of electromagnetic analyses. A novel antenna is presented for ultra high frequency (UHF) near-field radio frequency identification (RFID) applications. The reactance component of tag antenna considered chip impedance (-193j) is conjugated and matched for maximum power transmission. The antenna is fabricated by using Polyester (PET) dielectric substrate with permittivity 3.2. The size of antenna is 38 × 18 × 0.5 mm. The HFSS simulator is used for optimizing the proposed antenna. The antenna Return Loss is of 14.0 dB for the worst case in the middle of UHF range, better than 31.4 dB at 900 MHz.
Analyzing patterns of message passing (1988)

article Free Access Share on Analyzing patterns of message passing Authors: C. R. Ball MIT Artificial Intelligence Lab., Cambridge, MA MIT Artificial Intelligence Lab., Cambridge, MAView Profile , T. W. Leung Brown Univ., Providence, RI Brown Univ., Providence, RIView Profile , C. A. Waldspurger Xerox PARC, Palo Alto, CA Xerox PARC, Palo Alto, CAView Profile Authors Info & Claims ACM SIGPLAN NoticesVolume 24Issue 4April 1989 pp 191–193https://doi.org/10.1145/67387.67441Published:26 September 1988Publication History 2citation215DownloadsMetricsTotal Citations2Total Downloads215Last 12 Months6Last 6 weeks2 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my Alerts New Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
The Implications of a High Allelic Frequency of CYP2B6 G516T in Ethnic Chinese Persons (2006)

We investigate the luminosity functions of Lyman-break galaxies (LBG) at z~4 and 5 based on the optical imaging data obtained in the Subaru Deep Field Project. Three samples of LBGs in a contiguous 875 arcmin^2 area are constructed. One consists of 3,808 LBGs at z~4 down to i'=26.85 selected with the B-R vs R-i' diagram. The other two consist of 539 and 240 LBGs at z~5 down to z'=26.05 selected with two kinds of two-color diagrams: V-i' vs i'-z' and R-i' vs i'-z'. The adopted selection criteria are proved to be fairly reliable by spectroscopic observation. We derive the luminosity functions of the LBGs at rest-frame ultraviolet wavelengths down to M_{UV}=-19.2 at z~4 and M_{UV}=-20.3 at z~5. We find clear evolution of the luminosity function over the redshift range of 04.
C-Propeptides of Procollagens Iα1 and II that Differentially Accumulate in Enchondromas versus Chondrosarcomas Regulate Tumor Cell Survival and Migration (2010)

Chondrogenic tumors that exhibit benign or malignant behaviors synthesize variable amounts of cartilage-like extracellular matrix. To define the regulators of these phenotypes, we performed a proteomic comparison of multiple human chondrogenic tumors, which revealed differential accumulation of the C-propeptides of procollagens Ialpha1 and II (PC1CP and PC2CP) in malignant versus benign tumors, respectively. Expression patterns of PC1CP correlated with levels of tumor vascularization, whereas expression patterns of PC2CP suggested its susceptibility to immobilization within the extracellular matrix. Prompted by these observations, we investigated the functions of recombinant PC1CP and PC2CP in the extracellular matrix in soluble or immobilized states. Each induced beta1 integrin-mediated chondrocyte adhesion by distinct domains and efficacies, suggesting that they initiated distinct signaling pathways. Indeed, immobilized PC2CP, but not PC1CP, induced apoptosis of primary chondrocytes and EAhy926 endothelial cells. In contrast, soluble PC1CP, but not PC2CP, induced the migration of EAhy926 cells and increased vascular endothelial growth factor (VEGF) and CXCR4 expression in chondrocytes. Soluble PC2CP also increased VEGF expression, but along with a more pronounced effect on CXCR4 and matrix metalloproteinase 13 expression. Our findings suggest that PC1CP favors angiogenesis and tumor progression, but that PC2CP acts in a more complex manner, exerting antitumor and antiangiogenic properties through apoptosis induction when immobilized, but progression and metastasis when soluble. In summary, the relative levels of PC1CP and PC2CP and their interactions within the extracellular matrix contribute to tumor progression, angiogenesis, and metastasis in chondrogenic tumors.
Elucidation of Architectural Requirements from a Spacer in Supported Proline‐Based Catalysts of Enantioselective Aldol Reaction (2009)

Abstract In order to delineate the properties of the spacer architecture responsible for the strong positive dendritic effect exhibited by polymer‐supported proline‐based catalysts, we prepared two series of polystyrene‐bound model catalysts. The first series was based on a linear and partially dendritic spacers (of reduced branching and valency) imitating the length of the second generation spacer, while the second series was based on the first generation dendron spacer with one functional (proline‐terminated) and one non‐functional arm. Comparative studies of the model and original (fully dendritic) catalysts in the asymmetric aldol reaction of aromatic aldehydes with acetone disclose the features characteristic to the dendritic architecture, such as proximity between the terminal catalytic units and enhanced branching, as crucial for inducing higher yield and enantioselectivity in catalysis.
Immersion and dry ArF scanners enabling 22nm HP production and beyond (2012)

Pattern shrinks using multiple patterning techniques will continue to the 22nm half pitch (HP) node and beyond. The cutting-edge Nikon NSR-S621D immersion lithography tool, which builds upon the technology advancements of the NSR-S620D [1], was developed to satisfy the aggressive requirements for the 22 nm HP node and subsequent generations. The key design challenge for the S621D was to deliver further improvements to product overlay performance and CD uniformity, while also providing increased productivity. Since many different products are made within an IC manufacturing facility, various wafer process-related issues, including the flatness or grid distortion of the processed wafers and exposure-induced heating had to be addressed. Upgrades and enhancements were made to the S620D hardware and software systems to enable the S621D to minimize these process-related effects and deliver the necessary scanner performance. To enable continued process technology advancements, in addition to pattern shrinks at the most critical layers, resolution for less critical layers must also be improved proportionally. As a result, increased demand for dry ArF instead of KrF scanners is expected for less critical layers, and dry ArF tools are already being employed for some of these applications. Further, multiple patterning techniques, such as sidewall double patterning, actually enable use of dry ArF instead of immersion scanners for some critical layers having relaxed pattern resolution requirements. However, in order for this to be successful, the ArF dry tool must deliver overlay performance that is comparable to the latest generation immersion systems. Understanding these factors, an ArF dry scanner that has excellent overlay performance could be used effectively for critical layers and markedly improve cost of ownership (CoO). Therefore, Nikon has developed the NSR-S320F, a new dry ArF scanner also built upon the proven S620D Streamlign platform. By incorporating the Streamlign innovations, sufficient overlay accuracy for critical layers, as well as maximized productivity can be achieved. Furthermore, CoO will be significantly improved, which is the vital benefit when comparing ArF dry vs. immersion scanners. In this paper / presentation the latest S621D and S320F performance data will be introduced.
Experimental Investigation of the Effect of Subdicing on an Ultrasound Matrix Transducer (2021)

Over the past decades, real-time three-dimensional (3D) medical ultrasound has attracted much attention since it enables clinicians to diagnose more accurately. This calls for ultrasound matrix transducers with a large number of elements, which can be interfaced with an application-specific integrated circuit (ASIC) for data reduction. An important aspect of the design of such a transducer is the geometry of each element, since it affects the mode of vibration and, consequently, the efficiency of the transducer. In this paper, we experimentally investigate the effect of subdicing on a piezoelectric (PZT) transducer. We fabricate and acoustically characterize a prototype PZT matrix transducer built on top of ASICs. The prototype transducer contains subdiced and non-subdiced elements, whose performance can be directly compared under the same conditions. Measurement results show that subdiced elements have a better performance compared to non-subdiced ones. Subdicing increases the peak pressure by 25%, raises the bandwidth by 10% and reduces the ringing time by 25%.