1118 EFFECT OF MESCENCHYMAL DERIVED STEM CELL INFECTED WITH RECOMBINANT ADENOVIRUS EXPRESSING HUMAN BDNF ON THE ERECTILE FUNCTION IN THE RAT MODEL WITH CAVERNOUS NERVE INJURY (2011)

You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Basic Research1 Apr 20111118 EFFECT OF MESCENCHYMAL DERIVED STEM CELL INFECTED WITH RECOMBINANT ADENOVIRUS EXPRESSING HUMAN BDNF ON THE ERECTILE FUNCTION IN THE RAT MODEL WITH CAVERNOUS NERVE INJURY Su Jin Kim, Sang-Kuk Yang, Sang-Hoon Park, Young Chul Sung, Byung Il Yoon, Hyuk Jin Cho, Sung Hoo Hong, Ji Youl Lee, Tae-Kon Hwang, and Sae Woong Kim Su Jin KimSu Jin Kim Seoul, Korea, Republic of More articles by this author , Sang-Kuk YangSang-Kuk Yang Chungju-si, Choongchungbuk-do, Korea, Republic of More articles by this author , Sang-Hoon ParkSang-Hoon Park Pohang, Korea, Republic of More articles by this author , Young Chul SungYoung Chul Sung Pohang, Korea, Republic of More articles by this author , Byung Il YoonByung Il Yoon Seoul, Korea, Republic of More articles by this author , Hyuk Jin ChoHyuk Jin Cho Seoul, Korea, Republic of More articles by this author , Sung Hoo HongSung Hoo Hong Seoul, Korea, Republic of More articles by this author , Ji Youl LeeJi Youl Lee Seoul, Korea, Republic of More articles by this author , Tae-Kon HwangTae-Kon Hwang Seoul, Korea, Republic of More articles by this author , and Sae Woong KimSae Woong Kim Seoul, Korea, Republic of More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.715AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES There are many studies about the effect of growth factor on the erectile dysfunction (ED) as well as mescenchymal derived stem cell (MSC). Among the various growth factors, brain derived growth factor (BDNF) showed the effect on the nerve regeneration in major pelvic ganglion. Recently, recombinant adenoviruses expressing human BDNF (rAd/hBDNF) was constructed using the vector and transduction of rAd/hBDNF into MSCs was possibe. Therefore, we evaluate the role of MSCs infected with rAd/hBDNF on the erectile dysfunction in rat model with cavernous nerve injury. METHODS Twenty one male rats (250–300 gm) were used. The rats were devided as Group I (n=3, normal control), Group II (n=6, rats with cavernous nerve injury), Group III (n=6, rats with cavernous nerve injury and injection with MSC, 1x106 in 20 microliter) and Group IV (n=6, rats with cavernous nerve injury and injection with MSC, 1x106 in 20 microliter, infected with rAd/hBDNF). MSC was injected to the major pelvic ganglion (MPG) with or without rAd/hBDNF. After 4 weeks, Intracavernous pressure (ICP) and mean arterial pressure (MAP) were measured. PKH26 staining and Masson′s trichrome staining were performed. In addition, the level of BDNF in MPG and corpus cavernosum was evaluated. RESULTS After 4 weeks, ICP of Group III and IV were significantly increased compared with Group II (p<0.05). Moreover, ICP of Group IV was significantly increased compared with Group III (p<0.05). The differentiation of MSC was observed in major pelvic ganglion by PKH26 staining in Group III and IV. The smooth muscle was increased in Group III and IV compared with Group II by Masson′s trichrome staining. The amount of smooth muscle was similar in Group III and IV. The increase expression of BDNF was noted in Group IV. CONCLUSIONS The erectile function was more improved after injection with MSC infected with rAd/hBDNF in rat with ED caused by cavernous nerve injury. Therefore, the use of MSC infected with rAd/hBDNF may have more treatment effect on ED cause by cavernous nerve injury. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e449 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Su Jin Kim Seoul, Korea, Republic of More articles by this author Sang-Kuk Yang Chungju-si, Choongchungbuk-do, Korea, Republic of More articles by this author Sang-Hoon Park Pohang, Korea, Republic of More articles by this author Young Chul Sung Pohang, Korea, Republic of More articles by this author Byung Il Yoon Seoul, Korea, Republic of More articles by this author Hyuk Jin Cho Seoul, Korea, Republic of More articles by this author Sung Hoo Hong Seoul, Korea, Republic of More articles by this author Ji Youl Lee Seoul, Korea, Republic of More articles by this author Tae-Kon Hwang Seoul, Korea, Republic of More articles by this author Sae Woong Kim Seoul, Korea, Republic of More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ...