gebhart/patrepeval huggingface dataset:
Linearly chirped nanosecond stretched pulses from an extreme chirped pulse semiconductor mode-locked oscillator (2008)

An eXtreme Chirped Pulse Oscillator (XCPO) implemented with a Theta cavity and based on a semiconductor optical amplifier (SOA) is presented for generating 10ns frequency-swept pulses and 3.6ps compressed pulses directly from the oscillator. In this experiment, we show the two distinct characteristics of the XCPO which are the scalability of the output energy and the mode-locked spectrum. The laser cavity design allows for low repetition rate operation <100MHz, as well. The cavity, significantly, reduces nonlinear carrier dynamics, integrated self phase modulation (SPM), and fast gain recovery in an SOA. Due to the laser's ability to generate directly frequency-swept pulses from the oscillator, this oscillator can be used for high speed frequency-swept optical coherence tomography (OCT) and time-stretched photonic analog to digital converters (P-ADC).
Supplementary Methods from Multifactorial T-cell Hypofunction That Is Reversible Can Limit the Efficacy of Chimeric Antigen Receptor–Transduced Human T cells in Solid Tumors (2023)

&lt;p&gt;PDF file - 74K&lt;/p&gt;
Origin of aromatase inhibitory activity via proteochemometric modeling (2016)

Aromatase, the rate-limiting enzyme that catalyzes the conversion of androgen to estrogen, plays an essential role in the development of estrogen-dependent breast cancer. Side effects due to aromatase inhibitors (AIs) necessitate the pursuit of novel inhibitor candidates with high selectivity, lower toxicity and increased potency. Designing a novel therapeutic agent against aromatase could be achieved computationally by means of ligand-based and structure-based methods. For over a decade, we have utilized both approaches to design potential AIs for which quantitative structure-activity relationships and molecular docking were used to explore inhibitory mechanisms of AIs towards aromatase. However, such approaches do not consider the effects that aromatase variants have on different AIs. In this study, proteochemometrics modeling was applied to analyze the interaction space between AIs and aromatase variants as a function of their substructural and amino acid features. Good predictive performance was achieved, as rigorously verified by 10-fold cross-validation, external validation, leave-one-compound-out cross-validation, leave-one-protein-out cross-validation and Y-scrambling tests. The investigations presented herein provide important insights into the mechanisms of aromatase inhibitory activity that could aid in the design of novel potent AIs as breast cancer therapeutic agents.
Regulation of neuronal mRNA splicing and Tau isoform ratio by ATXN3 through deubiquitylation of splicing factors (2019)

Abstract The ubiquitylation/deubiquitylation balance in cells is maintained by deubiquitylating enzymes, including ATXN3. The precise role of this protein, mutated in spinocerebellar ataxia type 3 (SCA3), remains elusive, as few substrates for its deubiquitylating activity were identified. Here, we characterized the ubiquitome of neuronal cells lacking ATXN3, and found altered polyubiquitylation in a large proportion of proteins involved in RNA metabolism, including splicing factors. Using transcriptomic analysis and reporter minigenes we confirmed that splicing was globally altered in these cells. Among the candidate targets of ATXN3 was SRSF7 (9G8), a key regulator of MAPT (Tau) exon 10 splicing. Loss-of-function of ATXN3 led to reduced SRSF7 levels and a deregulation of MAPT exon 10 splicing, resulting in a decreased 4R/3R-Tau ratio. Similar alterations were found in cellular models of expanded polyQ ATXN3 and SCA3 patient brains, pointing to a relevant role of this mechanism in SCA3, and establishing a previously unsuspected link between two key proteins involved in different neurodegenerative disorders.
Human Bitter Taste Receptors Are Activated by Different Classes of Polyphenols (2018)

Polyphenols may contribute directly to plant-based foodstuffs flavor, in particular to astringency and bitterness. In this work, the bitterness of a small library of polyphenols from different classes [procyanidin dimers type B, ellagitannins (punicalagin, castalagin, and vescalagin) and phenolic acid ethyl esters (protocatechuic, ferulic, and vanillic acid ethyl esters] was studied by a cell-based assay. The bitter taste receptors (TAS2Rs) activated by these polyphenols and the half-maximum effective concentrations (EC<sub>50</sub>) of each agonist-TAS2Rs pair were determined. Computational methodologies were used to understand the polyphenol molecular region responsible for receptor activation and to get insights into the type of bonds established in the agonist-TAS2Rs pairs. The results show the combinatorial pattern of TAS2Rs activation. TAS2R5 seems to be the only receptor exhibiting a bias toward the activation by condensed tannins, while TAS2R7 seems more tuned for hydrolyzable (ellagi)tannins. Additionally, at the concentrations usually found for these compounds in foodstuffs, they can actively contribute to bitter taste, especially ellagitannins.
Metal Affinity Capture Tandem Mass Spectrometry for the Selective Detection of Phosphopeptides (2006)

We report a new method called metal affinity capture that when coupled with tandem mass spectrometry (MAC-MSMS) allows for the selective detection and identification of phosphopeptides in complex mixtures. Phosphopeptides are captured as ternary complexes with Ga(III) or Fe(III) and N(alpha),N(alpha)-bis(carboxymethyl)lysine (LysNTA) in solution and electrosprayed as doubly or triply charged positive ions. The gas-phase complexes uniformly dissociate to produce a common (LysNTA + H)+ ion that is used as a specific marker in precursor ion scans. The advantages of MAC-MSMS over the current methods of phosphopeptide detection are as follows. (1) MAC-MSMS uses metal complexes that self-assemble in solution at pH <5, which is favorable for the production of positive ions by electrospray. (2) Phosphorylation at tyrosine, serine, and threonine is detected by MAC-MSMS. (3) The phosphopeptide peaks in the mass spectra are encoded with the 69Ga-71Ga isotope pattern for selective recognition in mixtures. Detection by MAC-MSMS of singly and multiply phosphorylated peptides in tryptic digests is demonstrated at low-nanomolar protein concentrations.
Injectable Ceramic Microcast Silicon Carbonitride (SiCN) Microelectromechanical System (MEMS) for Extreme Temperature Environments with Extension: Micro Packages for Nano-Devices (2004)

MEMS that can survive ultrahigh-temperatures and corrosive environments are important in a variety of unique applications. This work develops an innovative microfabrication technology for high temperature MEMS: microcasting of injectable polymer-derived SiCN ceramic materials. The techniques allow realization of MEMS with complex, three-dimensional and multilayer geometry useable to 1500 C. Our approach is easy and low cost. Several MEMS were realized and evaluated. The objectives of the extension research project are to investigate a new micro-packaging technology to create a high-vacuum, sealed environment for nano-devices integrated with other electronic or RF circuits. The process fits standard manufacturing infrastructure, and can be scaled to package MEMS or nano devices. Such a micro-package will be critical to the advancement of devices such as nano sized resonators that require high vacuum and clean environment to maintain Q's on the order of 10,000 or higher, or very advanced RF MEMS where packaging can greatly affect performance. Two challenges were identified in the initial proposal: the precision creation of the micro-cap, and the deposition of glass droplets in vacuum. The final deliverable for this project was to demonstrate a MEMS sensor hermetically sealed in vacuum. The sensors performance was measured as a function of time to demonstrate the vacuum and hermetic sealing levels.
Ergonomic-Centric Holography: Optimizing Realism,Immersion, and Comfort for Holographic Display (2023)

We introduce ergonomic-centric holography, an algorithmic framework that simultaneously optimizes for realistic incoherent defocus, unrestricted pupil movements in the eye box, and high-order diffractions for filtering-free holography. The proposed method outperforms prior algorithms on holographic display prototypes operating in unfiltered and pupil-mimicking modes, offering the potential to enhance next-generation virtual and augmented reality experiences.
NLG vs. Templates (1995)

One of the most important questions in applied NLG is what benefits (or ‘valueadded’, in business-speak) NLG technology offers over template-based approaches. Despite the importance of this question to the applied NLG community, however, it has not been discussed much in the research NLG community, which I think is a pity. In this paper, I try to summarize the issues involved and recap current thinking on this topic. My goal is not to answer this question (I don’t think we know enough to be able to do so), but rather to increase the visibility of this issue in the research community, in the hope of getting some input and ideas on this very important question. I conclude with a list of specific research areas I would like to see more work in, because I think they would increase the ‘value-added’ of NLG over templates.
Aspects of Seismic Resolution: Chapter 1 (1985)

Where two or more reflections are more closely spaced than a quarter wavelength, a common situation for stratigraphic features of interest in petroleum exploration, the same half-cycles of the embedded wavelet tend to add; we call this the thin-bed case. In contrast, where they are close but separated by more than a quarter wavelength, different half-cycles tend to add; this is the case. Many characteristics of seismic reflections differ in thin- and thick-bed situations. On unmigrated seismic sections the limits of horizontal resolvability are imposed by Fresnel-zone considerations. However, on migrated sections other factors become important, such as noise on the unmigrated section, spatial aliasing, migration aperture, and uncertainties imposed by velocity, stacking, and two-dimensional assumptions.
Grafting polymer chains bearing an N‐succinimidyl activated ester end‐group onto primary amine‐coated silica particles and application of a simple, one‐step approach via nitroxide‐mediated controlled/living free‐radical polymerization (2009)

Abstract In a first part, the alkoxyamine initiator derived from BlocBuilder® based on the nitroxide SG1 and bearing an N ‐succinimidyl ester function was used to synthesize a variety of well‐defined polymers with the activated ester group at the α‐end. The grafting of those polymer chains onto primary‐amine functionalized Stöber silica particles was performed in soft conditions at room temperature and was studied as a function of the polymer chain length, the type of solvent, and the concentrations of both polymer and amine. Polymer grafting densities were mainly in the 0.1–0.2 chain nm −2 range. To increase the grafting density in simple experimental conditions, a direct one‐step method was then proposed: the primary‐amine modified silica, the N ‐succinimidyl ester functionalized alkoxyamine, and the monomer were all introduced into the reaction medium at once. This technique allowed grafting and chain growth to take place simultaneously at the polymerization temperature (i.e., 120 °C for styrene), in a single step, and produced hybrid particles with very high grafting density, up to 0.9 chain nm −2 , and long polymer chains. © 2009 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 48: 173–185, 2010
Stereochemical Effect Revealed in Self-Assemblies Based on Archaeal Lipid Analogues Bearing a Central Five-Membered Carbocycle: A SAXS Study (2012)

The relative stereochemistry (cis or trans) of a 1,3-disubstituted cyclopentane unit in the middle of tetraether archaeal bipolar lipid analogues was found to have a dramatic influence on their supramolecular self-assembly properties. SAXS studies of two synthetic diastereomeric archaeal lipids bearing two lactosyl polar head groups at opposite ends revealed different lyotropic behaviors. The cis isomer led to L(c)-L(α)-Q(II) transitions whereas the trans isomer retained an L(α) phase from 20 to 100 °C. These main differences originate from the conformational equilibrium (pseudorotation) of 1,3-disubstituted cyclopentanes. Indeed, this pseudorotation exhibits quite similar orientations of the two substituents in a trans isomer whereas several orientations of the two alkyl chains are expected in a cis-1,3-dialkyl cyclopentane, thus authorizing more conformational flexibility in the lipid packing.
The use of the surgical assistance system "ENDOFIX exo " in endoscopic ear surgery – first results (2023)

Introduction One-handed surgery is one of the challenges of endoscopic ear surgery (EES). This can be balanced by the intraoperative usage of surgical assistance systems, which allow a two-handed approach. We present a retrospective study investigating the worldwide first application of the passive endoscope holder "Endofix exo" (Fa. AKTORmed GmbH, Barbing, Germany) in EES by means of selected quality indices.
Adaptive QOS control for MPEG-4 video communication over wireless channels (2000)

This paper proposes an adaptive quality-of-service (QoS) control to increase the robustness of MPEG-4 video communication over wireless channels. More specifically, the proposed adaptive QoS control consists of optimal mode selection and delay-constrained hybrid automatic repeat request (ARQ). The optimal mode selection is employed to provide QoS support on the compression layer while delay-constrained hybrid ARQ is used to provide QoS support on the link layer. Simulation results show that the proposed adaptive QoS control achieves satisfactory quality for MPEG-4 video under dynamically changing wireless channel conditions and utilizes network resources efficiently.
A new insect cell line engineered to produce recombinant glycoproteins with cleavable N-glycans (2021)

Glycoproteins are difficult to crystallize because they have heterogeneous glycans composed of multiple monosaccharides with considerable rotational freedom about their O-glycosidic linkages. Crystallographers studying N-glycoproteins often circumvent this problem by using β1,2-N-acetylglucosaminyltransferase I (MGAT1)-deficient mammalian cell lines, which produce recombinant glycoproteins with immature N-glycans. These glycans support protein folding and quality control but can be removed using endo-β-N-acetylglucosaminidase H (Endo H). Many crystallographers also use the baculovirus-insect cell system (BICS) to produce recombinant proteins for their work but have no access to an MGAT1-deficient insect cell line to facilitate glycoprotein crystallization in this system. Thus, we used BICS-specific CRISPR-Cas9 vectors to edit the Mgat1 gene of a rhabdovirus-negative Spodoptera frugiperda cell line (Sf-RVN) and isolated a subclone with multiple Mgat1 deletions, which we named Sf-RVN<sup>Lec1</sup>. We found that Sf-RVN and Sf-RVN<sup>Lec1</sup> cells had identical growth properties and served equally well as hosts for baculovirus-mediated recombinant glycoprotein production. N-glycan profiling showed that a total endogenous glycoprotein fraction isolated from Sf-RVN<sup>Lec1</sup> cells had only immature and high mannose-type N-glycans. Finally, N-glycan profiling and endoglycosidase analyses showed that the vast majority of the N-glycans on three recombinant glycoproteins produced by Sf-RVN<sup>Lec1</sup> cells were Endo H-cleavable Man<sub>5</sub>GlcNAc<sub>2</sub> structures. Thus, this study yielded a new insect cell line for the BICS that can be used to produce recombinant glycoproteins with Endo H-cleavable N-glycans. This will enable researchers to combine the high productivity of the BICS with the ability to deglycosylate recombinant glycoproteins, which will facilitate efforts to determine glycoprotein structures by X-ray crystallography.
Consortium of Otolaryngology Journal Editors: collegiality and contributions (2020)

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Antimicrobial silver: An unprecedented anion effect (2014)

Silver is an indispensable metal but its use has to be minimised for sustainable growth. Much of the silver lost during use is unrecoverable; an example being its use as an antimicrobial agent, a property known since ages. While developing methods to create an affordable drinking water purifier especially for the developing world, we discovered that 50 parts per billion (ppb) of Ag(+) released continuously from silver nanoparticles confined in nanoscale cages is enough to cause antimicrobial activity in conditions of normal water. Here we show that the antibacterial and antiviral activities of Ag(+) can be enhanced ~1,000 fold, selectively, in presence of carbonate ions whose concentration was maintained below the drinking water norms. The protective layers of the organisms were affected during the carbonate-assisted antimicrobial activity. It is estimated that ~1,300 tons of silver can be saved annually using this new way to enhance its antimicrobial activity.
PRELOAD SENSITIVITY OF CONTINUOUS FLOW VENTRICULAR ASSIST DEVICES: APPLICATION TO THE TOTAL ARTIFICIAL HEART (2006)

Purpose: Pump design can affect pump sensitivity to preload. The effect of preload on the output of 3 axial flow ventricular assist devices (VADs) was studied in vitro. We hypothesize that slight changes in the design of axial flow pumps will alter the response to preload. Methods: The VADs studied included the HeartMate II, Jarvik 2000, and MicroMed DeBakey. The pump inflow cannulas of the HeartMate II and MicroMed DeBakey were removed so the pumps had direct interface with the testing fluid. The outlet of each pump was attached via a short tubing segment to a vertical PVC pipe with negligible flow resistance. Water column height in the PVC pipe determined pump afterload. Pump inlet and outlet pressures and flow rate were measured. To vary pump preload, the fluid in the preload cylinder was gradually drained while keeping water level in the afterload piping constant at 50, 70, or 90 mm Hg. Pressure and flow data were continuously measured at different pump speeds. Results: The flow-preload curves of the HeartMate II were nearly linear at all speeds. The Jarvik 2000 demonstrated increased sensitivity to preload at 11 and 12 krpm, with a maximum sensitivity of 0.35 L/min/mm Hg. The maximum sensitivity of the modified DeBakey pump was 2 L/min/mm Hg. Conclusions: Increasing preload sensitivity enhances the Starling-type autoregulatory mechanism of continuous flow pumps. We offer additional experimental evidence concerning this mechanism by using pumps with inherently different preload sensitivities.
Klinische Evaluation einer Software zur automatischen Lokalisation von Lungenrundherden in CT-Verlaufskontrollen (2004)

RAM is a valuable tool for follow-up of lung nodules at CT. Only very different inspiration levels influenced detection rate.
Stochastic Model Predictive Control for Quasi-Linear Parameter Varying Systems: Case Study on Automotive Engine Control (2022)

Abstract This paper presents an efficient stochastic model predictive control (SMPC) framework for quasi-linear parameter varying (qLPV) systems. The framework applies to general nonlinear systems that are driven by stochastic additive disturbances and subject to chance constraints. The qLPV form is featured by a composition of a set of linear time-invariant (LTI) models with state-/control-dependent scheduling variables, which can be obtained by the spatial–temporal filtering-based system identification approach developed in our earlier work. The overall framework can then be transformed into a tube-based MPC optimization problem which can be efficiently handled by a series of quadratic programing (QP) problems. A case study on automotive engine control is presented as a pilot demonstration of the proposed qLPV–SMPC where we show its advantage over the zone-based MPC, much greater computational efficiency than nonlinear MPC (NMPC) and less conservativeness of the proposed method as compared to its robust MPC (RMPC) counterpart.
Molecular and Biological Properties of Ionophores (1980)

The ionophores are a group of natural and synthetic compounds which form lipid-soluble cation complexes which can transport cations across low polarity barriers such as organic solvents and lipids (1). From a biological standpoint, the most important low polarity barrier is the lipid bilayer which lies within biological membranes; ionophores possess unique and potent biological properties which derive from their ability to perturb transmembrane ion gradients and electrical potentials. Each ionophore has its own characteristic ion selectivity pattern arising from the interaction between the conformational options of the host ionophore and the effective atomic radius and charge density of the guest cation. The ability of ionophores to complex and transport cations has an ever growing list of applications in experimental biology and technology and may ultimately provide the basis for novel cardiovascular drugs. Ionophores are also intriguing intellectually as objects for study of chemical and physical complexation processes at the
Substandard flight crew performance : recurrent human factors in flight crew initiated aircraft incidents and accidents (2017)

Substandard flight crew performance: recurrent human factors in flight crew initiated aircraft incidents and accidents
Evaluation of Fragmentation With Single or Multiple Pulse Setting of Lithoclast for Renal Calculi During Percutaneous Nephrolithotripsy and its Impact on Clearance (2004)

No AccessJournal of UrologyUrological Survey: ABSTRACTS: UROLITHIASIS, ENDOUROLOGY AND LAPAROSCOPY1 Aug 2004Evaluation of Fragmentation With Single or Multiple Pulse Setting of Lithoclast for Renal Calculi During Percutaneous Nephrolithotripsy and its Impact on Clearance Ralph V. ClaymanM.D. Ralph V. ClaymanRalph V. Clayman More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)61780-6AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "Evaluation of Fragmentation With Single or Multiple Pulse Setting of Lithoclast for Renal Calculi During Percutaneous Nephrolithotripsy and its Impact on Clearance." The Journal of Urology, 172(2), p. 800 References 1 : Laboratory and clinical assessment of pneumatically driven intracorporeal lithotripsy. J Endourol1998; 12: 163. Google Scholar © 2004 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 172 Issue 2 August 2004 Page: 800 Advertisement Copyright & Permissions© 2004 by American Urological Association, Inc.Metrics Author Information Ralph V. Clayman More articles by this author Expand All Advertisement PDF downloadLoading ...