gebhart/patrepeval huggingface dataset:
Abstract 2356: Erythrocytes used as tumor antigen delivery system to target antigen-presenting cells embody an innovative approach for in situ cancer immunotherapy (2016)

Abstract Introduction: In current vaccine therapy, efficient delivery of tumor antigens (TA) to antigen-presenting cells (APC) represents a major step toward the development of strong TA-specific immune response including cytotoxic T lymphocyte (CTL) induction. Our innovative approach is to use the property of erythrocytes to be naturally phagocytosed by APC when senescent. Indeed, TA can be encapsulated into erythrocytes that will be used as carrier to specifically deliver the TA to APC, which will ensure their degradation and presentation to T cells. Materials and Methods: The proof of concept was established with Ovalbumin (OVA), tyrosinase-related protein 2 (TRP2 (melanoma TA) and prostate specific antigen (PSA, prostate TA). These TA were encapsulated into erythrocytes by a hypotonic lysis process. To stimulate the erythrophagocytosis by APC, the membranes of erythrocytes were coated with antibodies. Then, erythrocytes containing TA were intravenously injected to mice concomitantly with Poly(I:C) adjuvant. The CTL response was analyzed by IFNγ ELISPOT and in vivo lysis of TA-expressing target cells injected to immunized mice. Humoral response was assessed by the determination of TA-specific immunoglobulin titer in mouse serum. Results: All TA were efficiently encapsulated in erythrocytes in a dose-dependent manner. A strong T-cell response was induced after 2 injections of TA encapsulated in erythrocytes: 2μg of OVA and 20μg of TRP2 induced the in vivo lysis of 97% and 96% of TA-target cells, respectively. By comparison, injections of free antigen induced less than 5% of lysis. Furthermore, a significant number of TA-specific IFNγ-secreting cells was generated (1585 and 286 cells / 106 cells for TRP2 and PSA, respectively) compared to the free TA (&amp;lt; 80 cells / 106 cells for both TA). In addition, we established a dose-dependent PSA-specific humoral response. Finally, a significant delay of tumor growth was obtained in mice more than twenty days after the subcutaneous implantation of EG7-OVA or B16F10 (melanoma) tumor cell lines, compared to injections with free antigen or control vehicle. Conclusion: The use of erythrocytes as TA carrier to specifically deliver antigen to APC and induce efficient immune response against tumor can be a very promising strategy in cancer immunotherapy. Citation Format: Magali Cremel, Nathalie Guerin, Quitterie Barthe, Vanessa Bourgeaux, Willy Berlier, Françoise Horand, Yann Godfrin. Erythrocytes used as tumor antigen delivery system to target antigen-presenting cells embody an innovative approach for in situ cancer immunotherapy. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 2356.
Electroluminescent device using two dimensional semiconductor (C/sub 6/H/sub 5/C/sub 2/H/sub 4/NH/sub 3)/sub 2/PpI/sub 4/ as an emitter (1994)

Summary form only given. Layered perovskite compounds (RNH/sub 3/)/sub 2/PbX/sub 4/ naturally form quantum-well structure; semiconducting /PbX/sub 4/ layers are separated by organic ammonium layers (RNH/sub 3/). The compounds possess attractive optical properties due to their low-dimensional semiconductor nature, for example, strong excitonic absorption, intense fluorescence from the exciton band, large third-order optical nonlinearity and so on. Further, the optical properties can be adjusted by chemical modification of (RNH/sub 3/) layer and replacing halogen X. In addition, the compounds are processable; one can obtain high-quality thin films of (RNH/sub 3/)/sub 2/PbX/sub 4/ by the spin-coating method. From the advantages, we expected the application of the compounds to electroluminescent (EL) devices. Using one of this type compounds, (C/sub 6/H/sub 5/C/sub 2/H/sub 4/NH/sub 3)/sub 2/PpI/sub 4/(PhEPbI4), we fabricated a multilayered electroluminescent (EL) device consisted of ITO anode, PhEPbI4 emitter layer, electron-transport layer of a oxadiazole derivative(OXD7) and MgAg cathode. PhEPb14 thin film was spin-coated on an ITO-coated glass substrate. Then, OXD7 layer and MgAg were vacuum-deposited successively. In a PhEPbI4 spin-coated film, a strong absorption due to exciton band observed around wavelength of 510 nm, demonstrating formation of layered perovskite structure. Further, green fluorescence with very narrow bandwidth from the exciton band was observed around 520 nm in the film. The bandwidth is less than 20 nm at room temperature. In the device, green EL with very narrow bandwidth was observed. The EL spectrum corresponded well to the fluorescence spectrum of PhEPbI4 thin film. Further, intense EL was attained in the device.
Macrophage-Targeted Photosensitizer Conjugate Delivered by Intratumoral Injection (2006)

A conjugate between maleylated albumin and a photosensitizer (PS) shows cell type specific targeting to macrophages via the scavenger receptor. Administration of this conjugate to a tumor-bearing mouse followed by illumination may allow selective destruction of macrophages within tumors. There is accumulating evidence that tumor-associated macrophages contribute to tumor growth, invasiveness, metastasis, and immune suppression. We tested the intravenous (IV) injection of a conjugate between maleylated albumin and chlorin(e6) to BALB/c mice bearing three tumor types with differing proportions of tumor-associated macrophages. The accumulation of PS within the tumors after IV injection and 24 h incubation time was disappointing, and we therefore investigated intratumoral (IT) injection. This gave 20-50 times greater concentrations of PS within the tumor compared to IV injection as determined by tissue extraction. Furthermore the amounts of PS in each tumor type correlated well with the numbers of macrophages both as determined by extraction from bulk tumor and fluorescence quantification and as determined by tissue dissociation to a single cell suspension and two-color flow cytometry with macrophage-specific antibodies. IT injection of nonconjugated PS gave lower tumor accumulation that did not correlate with macrophage content. IT injection of targeted macromolecular delivery systems is an underexplored area and worthy of further study.
Cardiovascular Effects of Long-Term Central and Peripheral Administration of Urocortin, Corticotropin-Releasing Factor, and Adrenocorticotropin in Sheep (2004)

The neuroendocrine hormones ACTH and corticotropin- releasing factor (CRF), which are involved in the stress response, have acute effects on arterial pressure. New evidence indicates that urocortin (UCN), the putative agonist for the CRF type 2 receptor, has selective cardiovascular actions. The responses to long-term infusions of these hormones, both peripherally and centrally, in conscious animals have not been studied. Knowledge of the long-term effects is important because they may differ considerably from their acute actions, and stress is frequently a chronic stimulus. The present experiments investigated the cardiovascular effects of CRF, UCN, and ACTH in conscious sheep. Infusions were made either into the lateral cerebral ventricles (i.c.v.) or i.v. over 4 d at 5 microg/h. UCN infused i.c.v. or i.v. caused a prolonged increase in heart rate (HR) (P < 0.01) and a small increase in mean arterial pressure (MAP) (P < 0.05). CRF infused i.c.v. or i.v. progressively increased MAP (P < 0.05) but had no effect on HR. Central administration of ACTH had no effect, whereas systemic infusion increased MAP and HR (P < 0.001). In conclusion, long-term administration of these three peptides associated with the stress response had prolonged, selective cardiovascular actions. The striking finding was the large and sustained increase in HR with i.c.v. and i.v. infusions of UCN. These responses are probably mediated by CRF type 2 receptors because they were not reproduced by infusions of CRF.
Towards Scalability of Quality Driven Semantic Web Service Composition (2009)

Optimizating semantic Web service compositions is known to be NP-hard, so most approaches restrict the number of services and offer poor scalability. We address the scalability issue by selecting compositions which satisfy a set of constraints rather than attempting to produce an optimal composition. Firstly, we define constraints within an innovative and extensible quality model designed to balance semantic fit (or functional quality) with quality of service (QoS) metrics. The semantic fit criterion evaluates the quality of semantic links between the semantic description of Web services parameters, whilst QoS focuses on non-functional criteria of services. Coupling these criteria allows us to further constrain and select valid compositions. To allow the use of this model in the context of millions of services as foreseen by the strategic EC-funded project SOA4All, we i) formulate the selection problem as a constraint satisfaction problem and ii) test the use of a stochastic search method. Finally we compare the latter with state-of-the-art approaches.
Recent evolution of renal replacement therapy in the critically ill patient. (2006)

The epidemiology of severe acute renal failure has dramatically changed in the past decade. Its leading cause is sepsis and the syndrome develops mostly in the intensive care unit as part of multiple organ dysfunction syndrome. After the significant improvements obtained from the mid 1970s to the mid 1990s, the past decade has seen a dramatic evolution in technology leading to new machines and new techniques for renal and multiple organ support. Extracorporeal therapies are now performed using adequate treatment doses, which have resulted in improved survival in the general population. At the same time, patients with sepsis seem to benefit from the use of increased doses, as in the case of high-volume hemofiltration or of increased membrane permeability and sorbents as in the case of continuous plasmafiltration adsorption. The humoral theory of sepsis and the peak concentration hypothesis have spurred a significant interest in the use of such extracorporeal therapies for renal support and possibly for the therapy of sepsis. Ongoing research and prospective studies will further elucidate the role of such therapies in this setting.
Genome Editing-Enabled HTS Assays Expand Drug Target Pathways for Charcot–Marie–Tooth Disease (2014)

Copy number variation resulting in excess PMP22 protein causes the peripheral neuropathy Charcot-Marie-Tooth disease, type 1A. To broadly interrogate chemically sensitive transcriptional pathways controlling PMP22 protein levels, we used the targeting precision of TALEN-mediated genome editing to embed reporters within the genetic locus harboring the Peripheral Myelin Protein 22 (Pmp22) gene. Using a Schwann cell line with constitutively high endogenous levels of Pmp22, we obtained allelic insertion of secreted bioluminescent reporters with sufficient signal to enable a 1536-well assay. Our findings from the quantitative high-throughput screening (qHTS) of several thousand drugs and clinically investigated compounds using this assay design both overlapped and expanded results from a previous assay using a randomly inserted reporter gene controlled by a single regulatory element of the Pmp22 gene. A key difference was the identification of a kinase-controlled inhibitory pathway of Pmp22 transcription revealed by the activity of the Protein kinase C (PKC)-modulator bryostatin.
Near-Unity Emitting, Widely Tailorable and Stable Exciton Concentrators Built from Doubly Gradient 2D Semiconductor Nanoplatelets (2023)

The strength of electrostatic interactions (EI) between electrons and holes within semiconductor nanocrystals profoundly impact the performance of their optoelectronic systems, and different optoelectronic devices demand distinct EI strength of the active medium. However, achieving a broad range, fine-tuning of the EI strength for specific optoelectronic applications is a daunting challenge, especially in quasi 2-dimensional core-shell semiconductor nanoplatelets (NPLs), as the epitaxial growth of the inorganic shell along the direction of the thickness that solely contributes to the quantum confined effect significantly undermines the strength of the EI. Herein we propose and demonstrate a novel doubly-gradient (DG) core-shell architecture of semiconductor NPLs for on-demand tailoring of the EI strength by controlling the localized exciton concentration via in-plane architectural modulation, demonstrated by a wide tuning of radiative recombination rate and exciton binding energy. Moreover, these exciton-concentration-engineered DG NPLs also exhibit a near-unity quantum yield, remarkable thermal and photo stability, as well as considerably suppressed self-absorption. As proof-of-concept demonstrations, highly efficient color converters and high-performance light-emitting diodes (external quantum efficiency: 16.9%, maximum luminance: 43,000 cd/m2) have been achieved based on the DG NPLs. This work thus opens up new avenues for developing high-performance colloidal optoelectronic device applications.
La récupération de l'énergie de la houle, partie 2 : systèmes de récupération et aspects électriques (2009)

National audience
Joint Air-Fuel Ratio and Torque Regulation Using Secondary Cylinder Air Flow Actuators (1999)

Actuation schemes exist that permit the joint management of air and fuel flow into the cylinders of a spark ignition engine. With the exception of drive by wire systems, to-date, the transient control aspects of these schemes, collectively refered to here as secondary cylinder air flow actuators, has not received any attention from the control community. This paper takes a first step in the analysis of the simultaneous dynamic control of air fuel ratio and torque response using secondary actuators placed before the intake ports of the cylinders, when used in combination with standard fuel injectors and primary throttle regulated by the driver. The emphasis is on basic issues of designing a feedforward scheme to enhance actuator authority for feedback control, and the fundamental multivariable nature of the feedback problem. Enhanced transient air-to-fuel ratio performance improvement is shown to be possible without sacrificing engine torque response with respect to a conventional engine. In addition, this is achieved with overall higher manifold pressure, offering the possibility of reduced pumping losses in the engine, depending on the actual actuation scheme employed.
4Pi microscopy with negligible sidelobes (2008)

The coherent addition of the wavefronts of two opposing high-angle lenses provides an axial (z) resolution improvement by 5–7-fold in far-field fluorescence microscopy. However, all microscopy concepts based on this principle have so far required mathematical deconvolution of the acquired data. This stems from the fact that the decrease of the axial width of the effective point spread function (EPSF) is accompanied by a substantial elevation of the side maxima of the EPSF along the optical axis. Here, we realize an EPSF with negligible lobes and gain axially superresolved images just through the physical phenomena involved. The constructive interference of the added wavefronts can be controlled through the image brightness which greatly simplifies the operation of the system.
Study of micro-scale limits of solder self-assembly for MEMS (2002)

Solder self-assembly of MEMS has been shown to be an excellent approach for assembling three-dimensional MEMS structures by allowing more precise alignments and vastly increased complexity. This paper investigates if solder self-assembly could be applied at the sub-micron scale. Experiments studying the surface energy minimization properties of molten solder demonstrate that surface energy minimized solder spheres as small as 5 nm are possible. However, the intermetallic formation may limit our ability to reach the nano-scales. Nevertheless, even with intermetallic formation, it should be possible to use solder self-assembly at sub micron scale.
A Novel Binning Algorithm Using Topic Modelling and k-mer Frequency on Groups of Non-Overlapping Short Reads (2020)

Metagenomics is a field that studies the microorganisms from the environment itself instead of traditional culturing methods. In this paper, we focus on the binning problem, which is to group reads into clusters that highly represent a taxonomic group. The result of this step serves as a crucial input for the next one of a metagenomic project such as assembly and annotation. Because metagenomic reads does not have explicit features, it is not easy to divide them into distinct groups. The solutions for this binning problem can be categorized as supervised and unsupervised approaches. Supervised ones need a reference database, which is unfortunately about 1% of the microorganisms in nature. This prevents these approaches from working well with the dataset that contain unknown species. In this paper we follow an unsupervised approach. Our proposed method is to combine the result from another technique named BiMeta, which based on a biological signature assumption that reads of a same taxonomic label have a same k-mer distribution, and topic modelling as a way of reducing the dimensions of the dataset. Our method shows better results (by precision, recall, and F-measure) than BiMeta on most datasets. Although following BiMeta, LDABiMeta out- performs it with the new proposed ideas. Moreover, our method is equiv- alent to MetaProb, which is the most successful method at present time, for the short-read datasets.
Evidence for the Importance of High-Valent FeO and of a Diketone in the Molecular Mechanism of Action of Antimalarial Trioxane Analogs of Artemisinin (1996)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTEvidence for the Importance of High-Valent FeO and of a Diketone in the Molecular Mechanism of Action of Antimalarial Trioxane Analogs of ArtemisininGary H. Posner, Sheldon B. Park, Lluïsa González, Dasong Wang, Jared N. Cumming, Donna Klinedinst, Theresa A. Shapiro, and Mario D. BachiView Author Information Department of Chemistry, School of Arts and Sciences, The Johns Hopkins University Baltimore, Maryland 21218 Department of Medicine, School of Medicine The Johns Hopkins University Baltimore, Maryland 21205 Department of Organic Chemistry The Weizmann Institute of Science, Rehovot, Israel Cite this: J. Am. Chem. Soc. 1996, 118, 14, 3537–3538Publication Date (Web):April 10, 1996Publication History Received8 December 1995Published online10 April 1996Published inissue 1 January 1996https://pubs.acs.org/doi/10.1021/ja954131phttps://doi.org/10.1021/ja954131prapid-communicationACS PublicationsCopyright © 1996 American Chemical SocietyRequest reuse permissionsArticle Views429Altmetric-Citations82LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information SUBJECTS:Antimicrobial agents,Carbon,Parasites,Rearrangement,Redox reactions Get e-Alerts
Intelligent Integrated Machining: Zr Thickness Measurements using XRF for Process Control and Quality Assurance (2014)

Reactor operation is dependent upon precise control of fuel plate geometry and thickness of individual layers, however there is currently no method to assess Zr diffusion barrier thickness over the entire surface area of the foil. An energy-dispersive x-ray fluorescence spectrometry (XRF) technique was developed to map Zr coating thickness over the surfaces of co-rolled fuel foils. Two different instruments and calibration methods were employed. An inexpensive handheld XRF analyzer was chosen for rapid non‐destructive estimation of Zr coating and/or Al cladding thickness in situ on the shop floor, while a laboratory-grade scanning macro-XRF was used to confirm the handheld measurements and for R&D applications. For Zr foil calibration standards, results from the handheld analyzer and the macro-XRF instrument are in excellent agreement with each other and within ±5% of known values. The method was successfully applied to map Zr on 48” LEU foils and to study effects of cold-rolling.
An Effective Foveated 360° Image Assessment Based on Graph Convolution Network (2022)

Virtual reality (VR) has been adopted in various fields such as entertainment, education, healthcare, and the military, due to its ability to provide an immersive experience to users. However, 360° images, one of the main components in VR systems, have bulky sizes and thus require effective transmitting and rendering solutions. One of the potential solutions is to use foveated technologies, that take advantage of the foveation feature of the human eyes. Foveated technologies can significantly reduce the data required for transmission and computation complexity in rendering. However, understanding the impact of foveated 360° images on human quality perception is still limited. This paper addresses the above problems by proposing an accurate machine-learning-based quality assessment model for foveated 360° images. The proposed model is proven to outperform the three cutting-edge machine-learning-based models, which apply deep learning techniques and 25 traditional-metric-based models (or analytical-function-based-models), which utilize analytical functions. It is also expected that our model helps to evaluate and improve 360° content streaming and rendering solutions to further reduce data sizes while ensuring user experience. Also, this model could be used as a building block to construct quality assessment methods for 360° videos, that are reserved for our future work. The source code is available at https://github.com/telagment/FoVGCN.
Aerosol generated mesoporous silica particles (2003)

Organized mesoporous silica sub-micro spheres have been synthesized by spray drying solutions containing hydrated Si(OEt)4 as silica precursor and surfactants as texturing agent. The resulting powder exhibits controlled organized 2-D hexagonal honey-comb like porosity of 2.2 nm mean pore diameter, homogeneous size dispersion (the particle average diameter is around 0.3 µm and standard deviation σ = 1.89), and a high specific surface area (1000 m2 g−1). The long-range order porosity was achieved after controlling different parameters: surfactant type and surfactant/TEOS ratios, dilution, pH, temperature and relative humidity under laminar flux motion conditions of aerosol generation. Powders were characterized using X-ray diffraction, TEM, N2 isotherm adsorption/desorption cycles and FTIR.