gebhart/patrepeval huggingface dataset:
Production of Recombinant β-Galactosidase in <i>Lactobacillus plantarum</i>, Using a pSIP-Based Food-Grade Expression System (2014)

Food-grade expression systems based on using food-grade microorganisms have been developed for the production of recombinant enzymes used in food applications. Lactic acid bacteria (LAB), especially Lactobacilli, have been widely used for various purposes in food and recognized as a promising host of food-grade enzyme production. In this study, the pSIP409 vectors, originally containing the erm gene, were used to replace this selection marker by the alr gene resulting in the production of the pSIP609 expression vector in L. planatarum . This vector could express high amounts of β-galactosidases, showing both high volumetric as well a specific enzymatic activity. Thus, the food-grade recombinant enzyme production in L. planatarum harboring pSIP609 was very fruitful and useful for food industries.
Role of p85 subunit of phosphatidylinositol-3-kinase as an adaptor molecule linking the insulin receptor to insulin receptor substrate 1. (1995)

After insulin stimulation of cells, signaling complexes are formed, containing the insulin receptor (IR), insulin receptor substrate-1 (IRS-1), and phosphatidylinositol-3-kinase. To study the nature of these complexes, we employed purified IR, recombinant IRS-1, antibodies to IR and IRS-1, and fusion proteins containing the two SH2 domains of p85. In intact cells, insulin increased tyrosine phosphorylation of both the IR and IRS-1. Both of these proteins were immunoprecipitated with antibodies to p85. Also, fusion proteins containing the two SH2 domains of p85 directly precipitated both the IR and IRS-1. Next, these signaling complexes were reconstituted in vitro with purified IR, recombinant IRS-1, and the two SH2 domains of p85. In the presence of both SH2 domains of p85, the IR associated with IRS-1. Other data, both in intact cells and in vitro, demonstrated that N- and C-terminal SH2 domains of p85 had preferential binding affinities for the IR and IRS-1, respectively. Studies with an IR mutant truncated in the C terminus indicated that the C-terminal phosphotyrosines of the IR play a major role in interacting with the SH2 domains of p85. In conclusion, both in vivo and in vitro data support a role for p85 in directly linking the IR to IRS-1 via its SH2 domains. The formation of these complexes, therefore, may provide a mechanism for the translocation to the plasma membrane of phosphatidylinositol-3-kinase and other molecules that are involved in IR signaling.
Micromachined Angular Rate Sensors for Automotive Applications (2007)

Micromachined angular rate sensors are key elements in several automotive systems, thus enabling highly sophisticated applications like rollover detection and mitigation, navigation systems, electronic stability program, and other future vehicle stabilizing and dynamics control systems. New automotive systems are demanding higher accuracy, better signal-to-noise ratio, higher robustness and insensitivity against external perturbations, better system availability and reliability, as well as easy application of the gyros. This paper is presenting the recent development, now the third generation, of micromachined angular rate sensors at Robert Bosch GmbH. Mass production was started in spring 2005. These surface micromachined gyroscopes exhibit outstanding performance compared to similar designs, especially in term of resolution, noise, and insensitivity against external perturbations
Pushing the limits of CMOS optical parametric amplifiers with USRN:Si7N3 above the two-photon absorption edge (2017)

Abstract CMOS platforms operating at the telecommunications wavelength either reside within the highly dissipative two-photon regime in silicon-based optical devices, or possess small nonlinearities. Bandgap engineering of non-stoichiometric silicon nitride using state-of-the-art fabrication techniques has led to our development of USRN (ultra-silicon-rich nitride) in the form of Si7N3, that possesses a high Kerr nonlinearity (2.8 × 10−13 cm2 W−1), an order of magnitude larger than that in stoichiometric silicon nitride. Here we experimentally demonstrate high-gain optical parametric amplification using USRN, which is compositionally tailored such that the 1, 550 nm wavelength resides above the two-photon absorption edge, while still possessing large nonlinearities. Optical parametric gain of 42.5 dB, as well as cascaded four-wave mixing with gain down to the third idler is observed and attributed to the high photon efficiency achieved through operating above the two-photon absorption edge, representing one of the largest optical parametric gains to date on a CMOS platform.
Transient elastography in anisotropic medium: Application to the measurement of slow and fast shear wave speeds in muscles (2003)

From the measurement of a low frequency (50-150 Hz) shear wave speed, transient elastography evaluates the Young's modulus in isotropic soft tissues. In this paper, it is shown that a rod source can generate a low frequency polarized shear strain waves. Consequently this technique allows to study anisotropic medium such as muscle. The evidence of the polarization of low frequency shear strain waves is supported by both numeric simulations and experiments. The numeric simulations are based on theoretical Green's functions in isotropic and anisotropic media (hexagonal system). The experiments in vitro led on beef muscle proves the pertinent of this simple anisotropic pattern. Results in vivo on man biceps shows the existence of slow and fast shear waves as predicted by theory.
Patch clamp technique: Review of the current state of the art and potential contributions from nanoengineering (2008)

The patch clamp technique permits high-resolution recording of the ionic currents flowing through a cell's plasma membrane. In different configurations, this technique has allowed experimenters to record and manipulate the currents that flow either through single ion channels or those that flow across the whole plasma membrane. Unfortunately, the conventional patch clamp method is laborious, requiring the careful fabrication of electrodes, skillful manipulation of the patch pipette towards a cell, and the clever design of electronics and apparatus to allow low-noise recordings. Advances in microfabrication offer promising technologies for high-throughput patch clamp recordings, particularly suitable for drug screening. This paper provides a review of the advances that have been made in the patch clamp technique over the years and considers where application of nanotechnology might provide significant contributions in the future.
Eradicating Health Care Disparities in the Surgical Management of ProstateCancer (2016)

Racial disparities in the arena of prostate cancer epidemiology and survival outcomes are conjectured to extend to the surgical management of this disease, particularly to the extent that African-American men encounter less favorable curative opportunities and disease control impact among racial groups in this regard. A literature review was performed to examine the utilization trends and therapeutic efficacy aspects of radical prostatectomy for clinically localized prostate cancer in African-American men. African-American men carry an adverse prostate cancer risk profile, with distinctive tumor biologic implications, although multiple risk factors across disease, patient, and provider domains are contributory. Delayed screening for this disease in African-American men, relative to Caucasian-American men, coupled with racial variations in patterns and quality of care, are highly relevant variables accounting for decreased radical prostatectomy utilization. Reports exist both in support of and in opposition to therapeutic efficacy equivalence among racial groups after radical prostatectomy. The racial variation in surgical care for prostate cancer can be addressed by applying both pattern and quality of care improvements in the delivery of surgical treatment. Future research advances will also be beneficial for advising optimal treatment based on targeted risk profiles.
Tetracycline Sensing Using Novel Doxycycline Derivatives Immobilized on Different Surface Plasmon Resonance Biosensor Surfaces (2010)

The novel doxycycline derivatives were successfully used as competitors in an indirect SPR assay.
A New Gorilla Adenoviral Vector with Natural Lung Tropism Avoids Liver Toxicity and Is Amenable to Capsid Engineering and Vector Retargeting (2020)

Human adenoviruses have many attractive features for gene therapy applications. However, the high prevalence of preexisting immunity against these viruses in general populations worldwide has greatly limited their clinical utility. In addition, the most commonly used human adenovirus, human adenovirus subgroup C serotype 5 (HAd5), when systemically administered, triggers systemic inflammation and toxicity, with the liver being the most severely affected organ. Here, we evaluated the utility and safety of a new low-seroprevalence gorilla adenovirus (GAd; GC46) as a gene transfer vector in mice. Biodistribution studies revealed that systemically administered GAd had a selective and robust lung endothelial cell (EC) tropism with minimal vector expression throughout many other organs and tissues. Administration of a high dose of GAd accomplished extensive transgene expression in the lung yet elicited no detectable inflammatory histopathology in this organ. Furthermore, GAd, unlike HAd5, did not exhibit hepatotropism or induce liver inflammatory toxicity in mice, demonstrating the exceptional safety profile of the vector vis-à-vis systemic utility. We further demonstrated that the GAd capsid fiber shared the flexibility of the HAd5 equivalent for permitting genetic modification; GAd with the pan-EC-targeting ligand myeloid cell-binding peptide (MBP) incorporated in the capsid displayed a reduced lung tropism and efficiently retargeted gene expression to vascular beds in other organs.<b>IMPORTANCE</b> In the aggregate, our mouse studies suggest that GAd is a promising gene therapy vector that utilizes lung ECs as a source of therapeutic payload production and a highly desirable toxicity profile. Further genetic engineering of the GAd capsid holds the promise of <i>in vivo</i> vector tropism modification and targeting.
Comparison of Rx-DSP-Structures in Experimental OFDMA-PON Uplink Transmission System (2014)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text J. von Hoyningen-Huene, H. Grießer, M. Eiselt, C. Ruprecht, and W. Rosenkranz, "Comparison of Rx-DSP-Structures in Experimental OFDMA-PON Uplink Transmission System," in Optical Fiber Communication Conference, OSA Technical Digest (online) (Optica Publishing Group, 2014), paper Tu2F.4. Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
Fiber Length and Orientation in Long-Fiber Injection-Molded Thermoplastics — Part I: Modeling of Microstructure and Elastic Properties (2008)

This article develops a methodology to predict the elastic properties of long-fiber injection-molded thermoplastics (LFTs). The corrected experimental fiber length distribution and the predicted and experimental orientation distributions were used in modeling to compute the elastic properties of the composite. First, from the fiber length distribution (FLD) data in terms of number of fibers versus fiber length, the probability density functions were built and used in the computation. The two-parameter Weibull's distribution was also used to represent the actual FLD. Next, the Mori—Tanaka model that employs the Eshelby's equivalent inclusion method was applied to calculate the stiffness matrix of the aligned fiber composite containing the established FLD. The stiffness of the actual as-formed composite was then determined from the stiffness of the computed aligned fiber composite that was averaged over all possible orientations using the orientation averaging method. The methodology to predict the elastic properties of LFTs was validated via experimental verification of the longitudinal and transverse moduli determined for long glass fiber injection-molded polypropylene specimens. Finally, a sensitivity analysis was conducted to determine the effect of a variation of FLD on the composite elastic properties. Our analysis shows that it is essential to obtain an accurate fiber orientation distribution and a realistic fiber length distribution to accurately predict the composite properties.
Scientific Process Automation and Workflow Management (2009)

We introduce and describe scientific workflows, i.e., executable descriptions of automatable scientific processes such as computational science simulations and data analyses. Scientific workflows are often expressed in terms of tasks and their (data ow) dependencies. This chapter first provides an overview of the characteristic features of scientific workflows and outlines their life cycle. A detailed case study highlights workflow challenges and solutions in simulation management. We then provide a brief overview of how some concrete systems support the various phases of the workflow life cycle, i.e., design, resource management, execution, and provenance management. We conclude with a discussion on community-based workflow sharing.
Optimum barrier layer for Al-PMD (preferential metal deposition) process (2003)

The barrier properties and the reliability of Al to Si contacts using different types of TiN in the Al-PMD process are investigated. The Al-PMD process is shown to be an excellent metallization technique for high density devices such as Giga-bit DRAMs and beyond when ACVD-TiN is used as a barrier layer. ACVD-TiN has the best diffusion barrier properties due to its high density and good conformality.
Diverse Patterns of Perilymphatic Space Enhancement in the Rat Inner Ear after Intratympanic Injection of Two Different Types of Gadolinium: A 9.4-Tesla Magnetic Resonance Study (2015)

We were able to clearly visualize and separate the ST and SV using IT Gd and 9.4-tesla micro-MRI. We recommend using Gd-DO3A-butrol over Gd-DOTA and to perform the MRI 2.5 h after using IT Gd in the rat inner ear.
An embedded DRAM for CMOS ASICs (2002)

The growing gap between on-chip gates and off-chip I/O bandwidth argues for ever larger amounts of on-chip memory. Emerging portable consumer technology, such as digital cameras, will also require more memory than can be supported easily on logic-oriented ASIC processes. Most ASIC memory systems are P-load SRAM, but this circuit technology is neither dense nor power efficient. This paper describes development of a DRAM, compatible with a standard CMOS ASIC process, that provides a memory density at least 4/spl times/ improved over P-load SRAM in the same layout roles. It runs at speeds comparable to logic in the same process and uses circuitry that is reasonably simple and portable. The design employs Vdd-precharge bit lines, half-capacitance full-voltage dummy cells, and a simple complementary sense amplifier. DRAM is organized as a number of small pages, allowing simple circuit design and low-power operation at modest expense in area overhead. The paper also described a power-conserving low-voltage-swing bus design that interfaces multiple pages to full-voltage-swing circuitry. Circuit and layout details are provided, along with experimental results for a 100 MHz 786K-bit embedded DRAM in a 0.5 /spl mu/m process.
Optimization of smart pixel receivers (2002)

The design of the optical receiver is an integral part of any optical interconnection scheme. The performance and information handling capacity of the free-space optical link depend to a large extent on the receiver's gain, speed, power consumption, and area requirements. In general, these four parameters can be traded off against each other. We have developed a framework for modeling receivers based on digital CMOS technology, and optimizing the design to minimize either the optical power requirement or the total power requirement.
miR‐212 is downregulated and suppresses methyl‐CpG‐binding protein MeCP2 in human gastric cancer (2009)

To clarify the role of micro (mi) RNAs in gastric carcinogenesis, we studied the expression and function of miRNAs in gastric carcinoma (GC) cells. Initially, we performed microarray analysis using total RNA from 3 human GC cell lines and noncancerous gastric tissue. Among the downregulated miRNAs in GC cells, miR-212 expression was decreased in all 8 GC cell lines examined and a significant decrease of miR-212 expression in human primary GC tissues was also observed in 6 of 11 cases. Transfection of the precursor miR-212 molecule induced decreased growth of 3 GC cell lines. Using 3 different databases, methyl-CpG-binding protein MeCP2 was postulated to be a target of miR-212. As seen on reporter assaying, miR-212 repressed the construct with the MECP2 3'-UTR. Ectopic expression of miR-212 repressed expression of the MeCP2 protein but not the MECP2 mRNA level. These data suggest that downregulation of miR-212 may be related to gastric carcinogenesis through its target genes, such as MECP2.
Formal Methods and Innovation Economy: Facing New Challenges (2008)

Formal methods (FM) are being actively developed nowadays. Still they find little use even in the target industry domains that require high quality, robust and standard-conforming software. Some of the difficulties in FM utilization are caused by unique characteristic features of the new Innovation Economy such as constantly growing number of new domains and technologies that calls for more and more experts who generate and possess knowledge. It is the knowledge formalization that is the prerequisite for FM application. This paper discusses features of the Innovation Economy that challenge FM practical use, considers positive cases of industrial applications of FM.Resting upon the analysis of those cases we suggest shifting the focus of FM development and extension to close cooperation with problem area experts and to promotion of the formalized approaches, techniques,and notations as interface language between problem areas and IT specialists.
Assessment of elastic parameters of human skin using dynamic elastography (2004)

Sonoelastography and transient elastography are two ultrasound-based techniques that facilitate noninvasive characterization of the viscoelastic properties of soft tissues by investigating their response to shear mechanical excitation. Young's modulus is the principle assessment parameter. Because it defines local tissue stiffness, it is of major interest for the medical imaging and cosmetic industries as it could replace subjective palpation by yielding local, quantitative information. In this paper, we describe a new high-resolution device capable of measuring local Young's modulus in very thin layers (1-5 mm) and devoted to the in vivo evaluation of the elastic properties of human skin. It uses an ultrasonic probe (50 MHz) for tracking the displacements induced by a 300 Hz shear wave generated by a ring surrounding the transducer. The displacements are measured using a conventional cross-correlation technique between successive ultrasonic back-scattered echoes. First, this noninvasive technique has been experimentally proven to be accurate for investigating elasticity in different skin-mimicking phantoms. Second, data were acquired in vivo on human forearms. As expected, Young's modulus was found to be higher in the dermis than in the hypodermis and other soft tissues.
Hybrid nano ridge plasmonic polaritons waveguides (2013)

We demonstrate an ultra-subwavelength surface plasmonic polaritons waveguide, which can confine light in the nano-scale region with comparable low propagation loss. The mode can be squeezed to one thousandth of the diffraction spot size with micro-meter scale propagation distance and is highly sensitive to the buffer layer materials and geometric parameters. This design improves the performance of previous surface plasmonic polaritons waveguides and lends itself to complementary metal–oxide–semiconductor compatible fabrication. These waveguides can be used as a platform for active devices as well as for nano-sensing applications.
<title>Steganographic system based on higher-order statistics</title> (2003)

Universal blind steganalysis attempts to detect steganographic data without knowledge about the applied steganographic system. Farid proposed such a detection algorithm based on higher-order statistics for separating original images from stego images. His method shows an astonishing performance on current steganographic schemes. Starting from the statistical approach in Farid's algorithm, we investigate the well known steganographic tool Jsteg as well as a newer approach proposed by Eggers et al., which relies on histogram-preserving data mapping. Both schemes show weaknesses leading to a certain detectability. Further analysis shows which statistic characteristics make both schemes vulnerable. Based on these results, the histogram preserving approach is enhanced such that it achieves perfect security with respect to Farid's algorithm.
Polymer–solid contacts described by soft, coarse-grained models (2011)

The ability of soft, coarse-grained models to describe the narrow interface of a nearly incompressible polymer melt in contact with a solid is explored by numerical self-consistent field calculations and Monte-Carlo simulations. We investigate the effect of the discreteness of the bead-spring architecture by quantitatively comparing the results of a bead-spring model with different number of beads, N, but identical end-to-end distance, R(e), and a continuous Gaussian-thread model. If the width, ξ, of the narrow polymer-solid contact is smaller or comparable to the length of a statistical segment, b=R(e)/√N-1, strong differences in the interface tension and the density profiles between the two models are observed, and strategies for compensating the discrete nature of the bead-spring model are investigated. Compensating the discretization of the chain contour in the bead-spring model by applying an external segment-solid potential, we simultaneously adjust the interface tension and the density profile to the predictions of the Gaussian-thread model. We suggest that the geometry of the polymer-solid contact and the interface tension are relevant characteristics that a coarse-grained model of polymer-solid contacts must reproduce in order to establish a quantitative relationship to an experimental system.
Nonlinearity Management and Compensation in Transmission Systems (2010)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text A. Pilipetskii, "Nonlinearity Management and Compensation in Transmission Systems," in Optical Fiber Communication Conference, OSA Technical Digest (CD) (Optica Publishing Group, 2010), paper OTuL1. Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article