gebhart/patrepeval huggingface dataset:
P2–067: Protein targets involved in the mechanisms of cell death induced by an amyloid precursor protein cytoplasmic subdomain (2006)

We previously demonstrated that the intraneuronal accumulation of the cytoplasmic juxtamembranar domain of amyloid precursor protein (APP) extending from the caspase cleavage site until the membrane (Jcasp) or until the gamma secretase site (Gcasp) induces apoptosis in vitro and in vivo. These domains are unmasked in the brains of Alzheimer patients. In these sequences the tyrosine 653 plays a crucial role as its mutation into aspartate or alanine abolishes the cell death observed. In order to elucidate the mechanisms of this cell death we focused our interest in the protein targets attached to the toxic domains detrimental to the endogenous APP or specifically recognized by the truncated cytoplasmic domains and responsible of the cell death observed. Large scale neuronal cultures were internalized with biotinylated Jcasp and Gcasp peptides and 3 hours later, cells were lysed and internalized peptides were recovered on streptavidin beads. Proteomic analysis of target proteins attached to these peptides were performed. Candidate partners were analyzed in regard of the physiological effect observed, using antisense strategy. First, we identified by proteomic analysis a new target of these APP sequences. This target is the SET protein also called template activating factor 1β or phosphatase 2A inhibitor 2 (I2PP2A), able to act as a transcription factor or as an activator of tau phosphorylation. Down regulation and overexpression experiments show that SET is directly involved in the cell death observed. Secondly, we detected by Western blotting PAT1 bound to the Jcasp peptide, and not to its mutated form. PAT1 is already known to interact with the APP juxtamembranar cytoplasmic domain. PAT1 is detected very early before SET, and is mainly present in the nucleus and at some extend in the cytoplasm. PAT1 and SET will be compared for their subcellular distributions in presence of the toxic peptides and their involvment in cell death. Taken together, these data can open new fields in the mechanisms of cell death observed in Alzheimer's disease.
YBa/sub 2/Cu/sub 3/O/sub 7/ thin films on nanocrystalline diamond films for HTSC bolometer (1993)

Superconducting YBa/sub 2/Cu/sub 3/O/sub 7-x/ films on nanocrystalline diamond thin films have been fabricated. A composite buffer layer system consisting of diamond/Si/sub 3/N/sub 4//YSZ/YBCO was explored for this purpose. The as-deposited YBCO films were superconducting with T/sub c/ of approximately 84 K and a relatively narrow transition width of approximately 8 K. Scanning electron microscope cross sections of the films showed very sharp interfaces between diamond/Si/sub 3/N/sub 4/ and between Si/sub 3/N/sub 4//YSZ. The deposited YBCO film has a surface roughness of approximately 1000 AA, which is suitable for high-temperature superconductive (HTSC) bolometer fabrication. It was also found that preannealing of the nanocrystalline diamond thin films at high temperature was very important for obtaining high-quality YBCO films.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Abstract 3764: Loss of β2SP (ELF) enhances the stem-like traits and invasiveness of HCC cells. (2013)

Abstract β2SP(ELF) is one of adapter proteins for Smad3/Smad4 complex formation during TGF-β signal transduction. 40% of β2SP+/- mice spontaneously develop hepatocellular carcinoma (HCC) and most cases of human HCC, gastric cancer, and lung cancer demonstrate significant reductions in β2SP expression. In our study, we investigated the possible mechanism for loss of β2SP induced HCC tumorigenesis. Recent studies have highlighted the involvement of cancer stem cells during the formation and progression of various types of cancers, epithelial cells that have undergone EMT (epithelial mesenchymal transition) acquire stem-cell properties during cancer development. Giving the role of TGF-beta signaling in stem cell differentiation and EMT during embryonic development, we hypothesize loss of β2SP results in alteration of liver stem cell that may contribute to the development of HCC in β2SP+/− mice. It has been shown that EpCAM-positive adult liver cells has stem cell property and can differentiate into functional hepatocytes in xenograft model. We found that EpCAM-positive hepatocyte number doubled in β2SP+/− mouse compared to wild type by fluorescence-activated cell sorter (FACS). Furthermore EpCAM level in β2SP+/− mouse liver was three times higher than that in the liver of wild type mouse. To study the mechanism by which Epcam is regulated by β2SP, HCC cells were transfected by β2SP siRNA. Inhibition of β2SP increased the expression of Epcam, and EMT markers vimentin was increased and E-cadherin expression was decreased in PLC/PRF/5 and SNU449 cells, while TGF-β1 and Smad3 rescued the expression of Epcam and E-cadherin, but not vimentin. We also found that suppression of β2SP expression promotes adhesion, migration and cloning formation of PLC/PRF/5 and SNU449 cells. Our data suggests loss of β2SP enhances the stem-like traits and invasiveness of HCC cells. Citation Format: Xiuling Zhi, Ling Lin, Narayan Shivapurkar, Wilma Jogunoori, Lopa Mishra, Aiwu R He. Loss of β2SP (ELF) enhances the stem-like traits and invasiveness of HCC cells. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 3764. doi:10.1158/1538-7445.AM2013-3764
Electronic spectra of pyrazolyl-bridged binuclear iridium(I) complexes (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectronic spectra of pyrazolyl-bridged binuclear iridium(I) complexesJanet L. Marshall, Michael D. Hopkins, Vincent M. Miskowski, and Harry B. GrayCite this: Inorg. Chem. 1992, 31, 24, 5034–5040Publication Date (Print):November 1, 1992Publication History Published online1 May 2002Published inissue 1 November 1992https://pubs.acs.org/doi/10.1021/ic00050a022https://doi.org/10.1021/ic00050a022research-articleACS PublicationsRequest reuse permissionsArticle Views129Altmetric-Citations33LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Transformer topologies for mmW integrated circuits (2009)

Layout topologies for millimeter-wave transformers are presented. Transformers whose windings present different shapes and relative position are compared in terms of inductance, quality-factors, coupling coefficient and minimum insertion loss. We present measurement results of transformers implemented in a 65 nm CMOS technology. It is observed that octagonal transformers present better Q-factors than square transformers, and that transformers whose primary is rotated by 180 degrees present a partial resonance.
Oxidative Stress Alters Syndecan-1 Distribution in Lungs with Pulmonary Fibrosis (2008)

Idiopathic pulmonary fibrosis (IPF) is an interstitial lung disease characterized by severe, progressive fibrosis. Roles for inflammation and oxidative stress have recently been demonstrated, but despite advances in understanding the pathogenesis, there are still no effective therapies for IPF. This study investigates how extracellular superoxide dismutase (EC-SOD), a syndecan-binding antioxidant enzyme, inhibits inflammation and lung fibrosis. We hypothesize that EC-SOD protects the lung from oxidant damage by preventing syndecan fragmentation/shedding. Wild-type or EC-SOD-null mice were exposed to an intratracheal instillation of asbestos or bleomycin. Western blot was used to detect syndecans in the bronchoalveolar lavage fluid and lung. Human lung samples (normal and IPF) were also analyzed. Immunohistochemistry for syndecan-1 and EC-SOD was performed on human and mouse lungs. In vitro, alveolar epithelial cells were exposed to oxidative stress and EC-SOD. Cell supernatants were analyzed for shed syndecan-1 by Western blot. Syndecan-1 ectodomain was assessed in wound healing and neutrophil chemotaxis. Increases in human syndecan-1 are detected in lung homogenates and lavage fluid of IPF lungs. Syndecan-1 is also significantly elevated in the lavage fluid of EC-SOD-null mice after asbestos and bleomycin exposure. On IHC, syndecan-1 staining increases within fibrotic areas of human and mouse lungs. In vitro, EC-SOD inhibits oxidant-induced loss of syndecan-1 from A549 cells. Shed and exogenous syndecan-1 ectodomain induce neutrophil chemotaxis, inhibit alveolar epithelial wound healing, and promote fibrogenesis. Oxidative shedding of syndecan-1 is an underlying cause of neutrophil chemotaxis and aberrant wound healing that may contribute to pulmonary fibrosis.
Roles of Endogenously Synthesized Sterols in the Endocytic Pathway (2006)

The effect(s) of endogenously synthesized cholesterol (endo-CHOL) on the endosomal system in mammalian cells has not been examined. Here we treated Chinese hamster ovary cell lines with lovastatin (a hydroxymethylglutaryl-CoA reductase inhibitor) and mevalonate (a precursor for isoprenoids) to block endo-CHOL synthesis and then examined its effects on the fate of cholesterol liberated from low density lipoprotein (LDL-CHOL). The results showed that blocking endo-CHOL synthesis for 2 h or longer does not impair the hydrolysis of cholesteryl esters but partially impairs the transport of LDL-CHOL to the plasma membrane. Blocking endo-CHOL synthesis for 2 h or longer also alters the localization patterns of the late endosomes/lysosomes and retards their motility, as monitored by time-lapse microscopy. LDL-CHOL overcomes the effect of blocking endo-CHOL synthesis on endosomal localization patterns and on endosomal motility. Overexpressing Rab9, a key late endosomal small GTPase, relieves the endosomal cholesterol accumulation in Niemann-Pick type C1 cells but does not revert the reduced endosomal motility caused by blocking endo-CHOL synthesis. Our results suggested that endo-CHOL contributes to the cholesterol content of late endosomes and controls its motility, in a manner independent of NPC1. These results also supported the concept that endosomal motility plays an important role in controlling cholesterol trafficking activities.
Structural Flexibility of the Linker Region of Human P-Glycoprotein Permits ATP Hydrolysis and Drug Transport (1998)

P-Glycoprotein (Pgp), an energy-dependent drug efflux pump responsible for multidrug resistance of many cancer cells, is comprised of two homologous halves connected by a peptide segment approximately 75 amino acids (aa) in length. The effects of length and composition of this connecting region on Pgp cell surface expression and the ability of the two halves to interact were explored using both stable transfections of Pgp mutants in mammalian cell lines and a vaccinia virus transient expression system. A 17 aa insertion of predicted flexible structure between amino acids 681 and 682 resulted in a functional Pgp molecule that was capable of conferring drug resistance. In contrast, an 18 aa peptide insertion with a predicted alpha-helical structure was unstable when expressed transiently. A 34 aa deletion from the central core of the linker region (Delta653-686) resulted in a protein expressed at the cell surface in amounts comparable to that of wild-type Pgp but unable to confer drug resistance. No apparent differences in drug or [alpha-32P]-8-azido-ATP photoaffinity labeling were observed. However, both ATP hydrolysis and drug transport activities of the deletion mutant were completely abrogated, indicating that the linker deletion disconnected substrate binding from ATP hydrolysis and transport. This mutant also failed to exhibit an ATP hydrolysis-dependent enhancement of binding of a conformation-sensitive monoclonal antibody, UIC2. Upon replacement with a 17 aa linker peptide having a predicted flexible secondary structure, but bearing no homology to the deleted 34 aa segment, normal Pgp transport and basal and drug-stimulated ATPase activities were restored along with increased UIC2 binding in the presence of substrate, suggesting a dramatic conformational change between the nonfunctional and functional molecules. Taken together, these data suggest a flexible secondary structure of the connector region is sufficient for the coordinate functioning of the two halves of Pgp, likely specifically required for the proper interaction of the two ATP binding sites.
Data Size-Aware Downlink Massive MIMO: A Session-Based Approach (2022)

This letter considers the development of transmission strategies for the downlink of massive multiple-input multiple-output networks, with the objective of minimizing the completion time of the transmission. Specifically, we introduce a session-based scheme that splits time into sessions and allocates different rates in different sessions for the different users. In each session, one user is selected to complete its transmission and will not join subsequent sessions, which results in successively lower levels of interference when moving from one session to the next. An algorithm is developed to assign users and allocate transmit power that minimizes the completion time. Numerical results show that our proposed session-based scheme significantly outperforms conventional non-session-based schemes.
Frequency-Domain Signal Processing for Spectrally-Enhanced CP-OFDM Waveforms in 5G New Radio (2021)

Orthogonal frequency-division multiplexing (OFDM) has been selected as the basis for the fifth-generation new radio (5G NR) waveform developments. However, effective signal processing tools are needed for enhancing the OFDM spectrum in various advanced transmission scenarios. In earlier work, we have shown that fast-convolution (FC) processing is a very flexible and efficient tool for filtered-OFDM signal generation and receiver-side subband filtering, e.g., for the mixed-numerology scenarios of the 5G NR. FC filtering approximates linear convolution through effective fast Fourier transform (FFT)-based circular convolutions using partly overlapping processing blocks. However, with the continuous overlap-and-save and overlap-and-add processing models with fixed block-size and fixed overlap, the FC-processing blocks cannot be aligned with all OFDM symbols of a transmission frame. Furthermore, 5G NR numerology does not allow to use transform lengths shorter than 128 because this would lead to non-integer cyclic prefix (CP) lengths. In this article, we present new FC-processing schemes which solve or avoid the mentioned limitations. These schemes are based on dynamically adjusting the overlap periods and extrapolating the CP samples, which make it possible to align the FC blocks with each OFDM symbol, even in case of variable CP lengths. This reduces complexity and latency, e.g., in mini-slot transmissions and, as an example, allows to use 16-point transforms in case of a 12-subcarrier-wide subband allocation, greatly reducing the implementation complexity. On the receiver side, the proposed scheme makes it possible to effectively combine cascaded inverse and forward FFT units in FC-filtered OFDM processing. Transform decomposition is used to simplify these computations, leading to significantly reduced implementation complexity in various transmission scenarios. A very extensive set of numerical results is also provided, in terms of the radio-link performance and associated processing complexity.
High Performance pMOSFETs with High Ge Fraction Strained SiGe-heterostructure-channel and Ultrashallow Source/Drain Formed by Selective B-Doped SiGe CVD (2006)

The improvement of current drivability and short channel effect is very important for ultrasmall MOS devices technology. SiGe-channel pMOSFETs are one of the most promising devices because hole mobility in the SiGe layers is enhanced. In the previous work, it has been reported that Super self-aligned shallow junction electrode (S3E) MOSFETs formed by selective B-doped SiGe CVD are effective for the suppression of short channel effect. In this paper, it is clarified that the (S3E) pMOSFETs with Si0.65Ge0.35-channel are realized not only with suppression of punch through due to the ultrashallow B-diffused source/drain but also with enhancement of maximum linear transconductance due to the low parasitic resistance, compared to that with the Si-channel fabricated by the same process conditions.
Tosca: Operationalizing Commitments Over Information Protocols (2017)

The notion of commitment is widely studied as a high-level abstraction for modeling multiagent interaction. An important challenge is supporting flexible decentralized enactments of commitment specifications. In this paper, we combine recent advances on specifying commitments and information protocols. Specifically, we contribute Tosca, a technique for automatically synthesizing information protocols from commitment specifications. Our main result is that the synthesized protocols support commitment alignment, which is the idea that agents must make compatible inferences about their commitments despite decentralization.
Supplementary Figure 3 from Stress Chaperone Mortalin Contributes to Epithelial-to-Mesenchymal Transition and Cancer Metastasis (2023)

&lt;p&gt;Mortalin overexpressing cells showed increase in microfilaments.&lt;/p&gt;
Data from EPHA2 Blockade Overcomes Acquired Resistance to EGFR Kinase Inhibitors in Lung Cancer (2023)

&lt;div&gt;Abstract&lt;p&gt;Despite the success of treating &lt;i&gt;EGFR&lt;/i&gt;-mutant lung cancer patients with EGFR tyrosine kinase inhibitors (TKI), all patients eventually acquire resistance to these therapies. Although various resistance mechanisms have been described, there are currently no FDA-approved therapies that target alternative mechanisms to treat lung tumors with acquired resistance to first-line EGFR TKI agents. Here we found that EPHA2 is overexpressed in EGFR TKI-resistant tumor cells. Loss of &lt;i&gt;EPHA2&lt;/i&gt; reduced the viability of erlotinib-resistant tumor cells harboring &lt;i&gt;EGFR&lt;/i&gt;&lt;sup&gt;T790M&lt;/sup&gt; mutations &lt;i&gt;in vitro&lt;/i&gt; and inhibited tumor growth and progression in an inducible &lt;i&gt;EGFR&lt;/i&gt;&lt;sup&gt;L858R+T790M&lt;/sup&gt;-mutant lung cancer model &lt;i&gt;in vivo&lt;/i&gt;. Targeting &lt;i&gt;EPHA2&lt;/i&gt; in erlotinib-resistant cells decreased S6K1-mediated phosphorylation of cell death agonist BAD, resulting in reduced tumor cell proliferation and increased apoptosis. Furthermore, pharmacologic inhibition of EPHA2 by the small-molecule inhibitor ALW-II-41-27 decreased both survival and proliferation of erlotinib-resistant tumor cells and inhibited tumor growth &lt;i&gt;in vivo&lt;/i&gt;. ALW-II-41-27 was also effective in decreasing viability of cells with acquired resistance to the third-generation EGFR TKI AZD9291. Collectively, these data define a role for EPHA2 in the maintenance of cell survival of TKI-resistant, &lt;i&gt;EGFR&lt;/i&gt;-mutant lung cancer and indicate that EPHA2 may serve as a useful therapeutic target in TKI-resistant tumors. &lt;i&gt;Cancer Res; 76(2); 305–18. ©2016 AACR&lt;/i&gt;.&lt;/p&gt;&lt;/div&gt;
Medial to posterior release procedure after failure of functional treatment in clubfoot: A prospective study (2016)

Level IV.
On the Importance of Trust in Next-Generation Networked CPS Systems: An AI Perspective (2021)

With the increasing scale, complexity, and heterogeneity of the next generation networked systems, seamless control, management, and security of such systems becomes increasingly challenging. Many diverse applications have driven interest in networked systems, including large-scale distributed learning, multi-agent optimization, 5G service provisioning, and network slicing, etc. In this paper, we propose trust as a measure to evaluate the status of network agents and improve the decision-making process. We interpret trust as a relation among entities that participate in various protocols. Trust relations are based on evidence created by the interactions of entities within a protocol and may be a composite of multiple metrics such as availability, reliability, resilience, etc. depending on application context. We first elaborate on the importance of trust as a metric and then present a mathematical framework for trust computation and aggregation within a network. Then we show in practice, how trust can be integrated into network decision-making processes by presenting two examples. In the first example, we show how utilizing the trust evidence can improve the performance and the security of Federated Learning. Second, we show how a 5G network resource provisioning framework can be improved when augmented with a trust-aware decision-making scheme. We verify the validity of our trust-based approach through simulations. Finally, we explain the challenges associated with aggregating the trust evidence and briefly explain our ideas to tackle them.
Flexible tuning concept for fiber-integrated lasers featuring multi-wavelength emission with fast switching speeds for DIAL (2019)

Based on favorable properties with respect to beam quality, efficiency and applicability, fiber-integrated lasers replace costly bulk lasers in various application fields, such as in academia, industry, medical technology and life sciences. Additionally, rare-earth doped fibers exhibit broad gain regions, providing huge potential to develop broadly tunable fiber lasers for spectroscopy. Recently a fiber-integrated tuning concept based on an FBG array as discrete spectral filter was demonstrated, exhibiting a unique flexibility to tailor spectral and temporal emission properties. In this work, we present the prospects of this concept to address Differential Absorption LIDAR (DIAL) for environmental sensing. With tunable multi-wavelength operation for enhanced measurement speeds, and customized spectral emission lines to probe specific molecules, it may provide a fast and cost-efficient solution with excellent usability.
New anode design concept of 600V thin wafer PT-IGBT with very low dose p-buffer and transparent p-emitter (2004)

We propose 600V new thin wafer PT (Punch Through) IGBT having a new concept of anode design. This proposed PT-IGBT has a very low dose p-type layer, called p-buffer, between a transparent p-emitter (anode) and an n-buffer layer. This provides a practical design for easy fabrication without deteriorating the good feature of the thin wafer PT-IGBTs. The n-buffer dose and the p-emitter dose can be precisely controlled by the doses of the two ion implantations. This is great merit in precise control of the p-emitter injection efficiency. An oscillation in the turn-off waveforms also disappears for the proposed PT-IGBT with p-buffer layer, because a smooth turn-off is achieved by a small tail current. The total power loss is not affected by the small tail loss.