gebhart/patrepeval huggingface dataset:
Effects of tunicamycin on insect cells (1980)

Conference Article| April 01 1980 Effects of tunicamycin on insect cells T. D. BUTTERS; T. D. BUTTERS 1National Institute for Medical Research, Mill Hill, London NW7 1AA, U.K. Search for other works by this author on: This Site PubMed Google Scholar R. C. HUGHES R. C. HUGHES 1National Institute for Medical Research, Mill Hill, London NW7 1AA, U.K. Search for other works by this author on: This Site PubMed Google Scholar Author and article information Publisher: Portland Press Ltd Online ISSN: 1470-8752 Print ISSN: 0300-5127 © 1980 Biochemical Society1980 Biochem Soc Trans (1980) 8 (2): 170–171. https://doi.org/10.1042/bst0080170 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation T. D. BUTTERS, R. C. HUGHES; Effects of tunicamycin on insect cells. Biochem Soc Trans 1 April 1980; 8 (2): 170–171. doi: https://doi.org/10.1042/bst0080170 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: SDS, sodium dodecyl sulphate, Con A, concanavalin A, SBA, soya-bean agglutinin This content is only available as a PDF. © 1980 Biochemical Society1980 Article PDF first page preview Close Modal You do not currently have access to this content.
The 28-kDa protein whose phosphorylation is induced by protein kinase C activators in MCF-7 cells belongs to the family of low molecular mass heat shock proteins and is the estrogen-regulated 24-kDa protein (1993)

We have previously reported the presence of a 28-kDa protein in human mammary adenocarcinoma MCF-7 cells, whose phosphorylation by phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) and permeant diacylglycerol 1,2-dioctanoyl-sn-glycerol was correlated to growth arrest induced by the protein kinase C (PKC) activators. We now investigate the possible identity of this protein with the estrogen-regulated "24-kDa" protein shown as related to the mammalian heat shock protein 27 (Fuqua, S. A. W., Blum-Salingaros, M., and McGuire, W. L. (1989) Cancer Res 49, 4126-4129). 32P-Labeled 28-kDa protein from TPA-treated MCF-7 cells was immunoprecipitated with a 24-kDa-specific monoclonal antibody. Immunoblots from cell extracts fractionated by two-dimensional isoelectric focusing/SDS-polyacrylamide gel electrophoresis demonstrated that TPA induced the conversion of a 28-kDa isoform "a" (pI 6.7) to a more acidic isoform "b" (pI 6.2). Two-dimensional gel analysis of [3H]leucine-labeled MCF-7 cell extracts demonstrated that conversely to TPA, which induced only phosphorylation of 28-kDa protein, heat shock induced both synthesis (increase of isoform a) and phosphorylation (conversion of isoforms a to b) of the protein. 32P labeling of MCF-7 cells allowed demonstration of the presence of an extra phosphoisoform "c" (pI 5.9) upon TPA as well as heat shock treatment. When cells were pretreated with the bisindolylmaleimide GF109203X, a selective inhibitor of PKC, the heat shock-induced phosphorylation was unchanged, while the TPA effect was almost abolished, suggesting that the heat shock-activated protein kinase was very likely different from PKC. However, peptide mapping of the 28-kDa phosphoprotein suggested identical sites of phosphorylation upon TPA and heat shock stimulation. Partial amino acid sequencing of the 28-kDa protein revealed identity with both the 24-kDa protein and the mammalian HSP27. The fact that estrogens and PKC, respectively, regulate expression and phosphorylation of this 24/28-kDa protein strongly argues for its key role in MCF-7 cell proliferation and differentiation.
High Incidence of Preharvest Colonization of Huanglongbing-Symptomatic Citrus sinensis Fruit by Lasiodiplodia theobromae (Diplodia natalensis) and Exacerbation of Postharvest Fruit Decay by That Fungus (2014)

Huanglongbing (HLB), presumably caused by the bacterium "Candidatus Liberibacter asiaticus," is a devastating citrus disease associated with excessive preharvest fruit drop. Lasiodiplodia theobromae (diplodia) is the causal organism of citrus stem end rot (SER). The pathogen infects citrus fruit under the calyx abscission zone (AZ-C) and is associated with cell wall hydrolytic enzymes similar to plant enzymes involved in abscission. By means of DNA sequencing, diplodia was found in "Ca. Liberibacter asiaticus"-positive juice from HLB-symptomatic fruit (S) but not in "Ca. Liberibacter asiaticus"-negative juice. Therefore, the incidence of diplodia in fruit tissues, the impact on HLB-related postharvest decay, and the implications for HLB-related preharvest fruit drop were investigated in Hamlin and Valencia oranges. Quantitative PCR results (qPCR) revealed a significantly (P < 0.001) greater incidence of diplodia in the AZ-C of HLB-symptomatic (S; "Ca. Liberibacter asiaticus" threshold cycle [CT] of <30) than in the AZ-C of in asymptomatic (AS; "Ca. Liberibacter asiaticus" CT of ≥30) fruit. In agreement with the qPCR results, 2 weeks after exposure to ethylene, the incidences of SER in S fruit were 66.7% (Hamlin) and 58.7% (Valencia), whereas for AS fruit the decay rates were 6.7% (Hamlin) and 5.3% (Valencia). Diplodia colonization of S fruit AZ-C was observed by scanning electron microscopy and confirmed by PCR test and morphology of conidia in isolates from the AZ-C after surface sterilization. Diplodia CT values were negatively correlated with ethylene production (R = -0.838 for Hamlin; R = -0.858 for Valencia) in S fruit, and positively correlated with fruit detachment force (R = 0.855 for Hamlin; R = 0.850 for Valencia), suggesting that diplodia colonization in AZ-C may exacerbate HLB-associated preharvest fruit drop.
Large-scale manufacturing and characterization of CMV-CD19CAR T cells (2022)

We demonstrated the feasibility of our large-scale platform for generating CMV-CD19CAR T cells for clinical application. We plan to initiate a clinical trial at City of Hope using CMV-CD19CAR T cells for patients with intermediate/high-grade B cell non-Hodgkin's lymphoma immediately after autologous hematopoietic cell transplantation followed by vaccination with a novel CMV vaccine based on Modified Vaccinia Ankara (Triplex) 28 days and 56 days post-T cell infusion.
Copolymerization of ethylene with aromatic vinyl monomers using metallocenes (2001)

Abstract Copolymerization of ethylene with styrene, allyl benzene and 4‐phenyl‐1‐butene was examined using different metallocene catalysts. We separated the bulky phenyl group of styrene from its double bond using methylene spacers and studied the effect on its copolymerization behaviour with ethylene. The extent of incorporation of the comonomer is in the order 4‐phenyl‐1‐butene &gt; allyl benzene &gt; styrene for all catalysts. The comonomer incorporation was found to be less than 10 mol% under the experimental conditions employed in the present study. © 2001 Society of Chemical Industry
In vivo mutation assay based on the endogenous Pig‐a locus (2008)

The product of the X-chromosome's Pig-a gene acts in the first step of glycosylphosphatidylinositol (GPI) anchor biosynthesis, and is thereby essential for attaching certain proteins to the cell surface. The experiments described herein were designed to evaluate whether lack of GPI-anchored proteins could form the basis of an in vivo mutation assay. Specifically, we used a CD59-negative cell surface phenotype to denote Pig-a mutation. Besides anti-CD59-PE, two other fluorescent reagents were used: thiazole orange to differentiate mature erythrocytes, reticulocytes (RETs), and leukocytes; and anti-CD61 to resolve platelets. These experiments were performed with Sprague Dawley rats, and focused on two cell populations, total erythrocytes and RETs. The ability of the analytical method to enumerate CD59-negative erythrocytes was initially assessed with reconstruction experiments whereby mutant-mimicking cells were added to control bloods. Subsequently, female rats were treated on three occasions with the model mutagens ENU (100 mg/kg/day) or DMBA (40 mg/kg/day). Blood specimens were harvested at various intervals, as late as 6 weeks post-exposure. Considering all week 4-6 data, we found that CD59-negative cells ranged from 239 to 855 x 10(-6) and 82 to 405 x 10(-6) for ENU and DMBA, respectively. These values were consistently greater than those observed for negative control rats (18 +/- 19 x 10(-6)). The elevated frequencies observed for the genotoxicant-exposed animals were usually higher for RETs compared to total erythrocytes. These data support the hypothesis that an efficient in vivo mutation assay can be developed around flow cytometric enumeration of erythrocytes and/or RETs that exhibit aberrant GPI-anchored protein expression.
Active Microelectronic Neurosensor Arrays for Implantable Brain Communication Interfaces (2009)

We have built a wireless implantable microelectronic device for transmitting cortical signals transcutaneously. The device is aimed at interfacing a cortical microelectrode array to an external computer for neural control applications. Our implantable microsystem enables 16-channel broadband neural recording in a nonhuman primate brain by converting these signals to a digital stream of infrared light pulses for transmission through the skin. The implantable unit employs a flexible polymer substrate onto which we have integrated ultra-low power amplification with analog multiplexing, an analog-to-digital converter, a low power digital controller chip, and infrared telemetry. The scalable 16-channel microsystem can employ any of several modalities of power supply, including radio frequency by induction, or infrared light via photovoltaic conversion. As of the time of this report, the implant has been tested as a subchronic unit in nonhuman primates ( approximately 1 month), yielding robust spike and broadband neural data on all available channels.
Discovery of a Potent and Selective PI3Kδ Inhibitor (S)-2,4-Diamino-6-((1-(7-fluoro-1-(4-fluorophenyl)-4-oxo-3-phenyl-4H-quinolizin-2-yl)ethyl)amino)pyrimidine-5-carbonitrile with Improved Pharmacokinetic Profile and Superior Efficacy in Hematological Cancer Models (2020)

PI3Kδ inhibitors have been approved for B-cell malignancies like CLL, small lymphocytic lymphoma, and so forth. However, currently available PI3Kδ inhibitors are nonoptimal, showing weakness against at least one of the several important properties: potency, isoform selectivity, and/or pharmacokinetic profile. To come up with a PI3Kδ inhibitor that overcomes all these deficiencies, a pharmacophoric expansion strategy was employed. Herein, we describe a systematic transformation of a "three-blade propeller" shaped lead, 2,3-disubstituted quinolizinone <b>11</b>, through a 1,2-disubstituted quinolizinone <b>20</b> to a novel "four-blade propeller" shaped 1,2,3-trisubstituted quinolizinone <b>34</b>. Compound <b>34</b> has excellent potency, isoform selectivity, metabolic stability across species, and exhibited a favorable pharmacokinetic profile. Compound <b>34</b> also demonstrated a differentiated efficacy profile in human germinal center B and activated B cell-DLBCL cell lines and xenograft models. Compound <b>34</b> qualifies for further evaluation as a candidate for monotherapy or in combination with other targeted agents in DLBCLs and other forms of iNHL.
Effects of sample preparation on cyclosporin G (NVA2-cyclosporin) concentration. (1987)

Journal Article Effects of sample preparation on cyclosporin G (NVA2-cyclosporin) concentration. Get access R W Yatscoff, R W Yatscoff Search for other works by this author on: Oxford Academic Google Scholar J R Jeffrey J R Jeffrey Search for other works by this author on: Oxford Academic Google Scholar Clinical Chemistry, Volume 33, Issue 7, 1 July 1987, Page 1257, https://doi.org/10.1093/clinchem/33.7.1257 Published: 01 July 1987
Transforming Cartesian Coordinates X, Y, Z to Geographical Coordinates φ, λ, h (1999)

Abstract In November 1994, the Intergovernmental Committee on Surveying and Mapping (ICSM) adopted a new geodetic datum for Australia and recommended its progressive implementation nationally. This datum, designated Geocentric Datum of Australia (GDA) - compatible with the Global Positioning System (GPS), a US Department of Defense satellite positioning system - will eventually replace the existing Australian Geodetic Datum (AGD). Consequently, "old" AGD coordinates need to be converted to "new" GDA coordinates, which may require the transformation of Cartesian coordinates X,Y,Z to geographical coordinates φ, λ, h (latitude, longitude, height) related to an ellipsoid of revolution. Given X,Y,Z, longitude is easily derived, but not so latitude, which requires more sophisticated evaluation - usually by iterative techniques, or complicated direct methods - the height following readily once latitude is obtained. Thus Cartesian-to-geographic transformations revolve around the determination of latitude; this paper reviews published techniques, some quite recent, which may be of use to practitioners.
Urban data collectors: A pragmatic approach to leveraging urban sensing (2015)

This paper proposes the adoption of a more pragmatic approach to urban sensing to leverage the initial offering of open databases within the context of smart cities. In this sense, the use of mobility agents already available to provide mobility to traditional sensors can enable a preliminary instrumentation of cities more quickly and possibly at a lower cost. As proof of concept, we investigated the use of metropolitan bus equipped with sensors such as urban data collectors. We believe that this strategy can help overcoming many challenges of traditional approaches for instrumentation of cities, such as power supply, maintenance, operation, and protection of a large amount of static sensors. To assess the feasibility of sampling by collectors, we conducted experiments comparing averages using frequent readings vs averages using intermittent readings collected by sensors of a climatological micro station.
Pharmacokinetics of Upadacitinib With the Clinical Regimens of the Extended‐Release Formulation Utilized in Rheumatoid Arthritis Phase 3 Trials (2018)

Upadacitinib is a Janus kinase 1 inhibitor under development for the treatment of several inflammatory disorders including rheumatoid arthritis (RA). Upadacitinib was administered in the phase 2 RA trials primarily as twice-daily regimens of an immediate-release (IR) formulation. The upadacitinib extended-release (ER) formulation was developed to enable once-daily dosing. In the present study, upadacitinib pharmacokinetics were characterized after the administration of single and multiple once-daily doses of the ER formulation in healthy subjects relative to single and multiple twice-daily doses of the IR formulation. Increase in upadacitinib exposure was dose-proportional over the evaluated 15- to 30-mg ER dose range. Single 15- and 30-mg ER doses provided equivalent AUC<sub>0-inf</sub> compared with single 12- and 24-mg IR doses, respectively. A high-fat breakfast increased upadacitinib ER C<sub>max</sub> and AUC<sub>0-inf</sub> by only 20% and 17%, respectively, relative to fasting conditions. The median time to peak plasma concentrations was 2 to 4 hours for the ER formulation, and steady state was achieved by day 4 of once-daily dosing. Doses of 15 and 30 mg once daily using the ER formulation provided equivalent AUC<sub>0-24</sub> , comparable C<sub>max</sub> and C<sub>min</sub> , and a fluctuation index over a 24-hour period at steady state similar to 6 and 12 mg twice daily, respectively, using the IR formulation. These results supported the use of upadacitinib 15- and 30-mg doses of the ER formulation in the phase 3 trials in RA.
Graphical criteria for positive solutions to linear systems (2017)

We study linear systems of equations with coefficients in a generic partially ordered ring $R$ and a unique solution, and seek conditions for the solution to be nonnegative, that is, every component of the solution is a quotient of two nonnegative elements in $R$. The requirement of a nonnegative solution arises typically in applications, such as in biology and ecology, where quantities of interest are concentrations and abundances. We provide novel conditions on a labeled multidigraph associated with the linear system that guarantee the solution to be nonnegative. Furthermore, we study a generalization of the first class of linear systems, where the coefficient matrix has a specific block form and provide analogous conditions for nonnegativity of the solution, similarly based on a labeled multidigraph. The latter scenario arises naturally in chemical reaction network theory, when studying full or partial parameterizations of the positive part of the steady state variety of a polynomial dynamical system in the concentrations of the molecular species.
Robustness Analysis of Face Obscuration (2020)

Face obscuration is needed by law enforcement and mass media outlets to guarantee privacy. Sharing sensitive content where obscuration or redaction techniques have failed to completely remove all identifiable traces can lead to many legal and social issues. Hence, we need to be able to systematically measure the face obscuration performance of a given technique. In this paper we propose to measure the effectiveness of eight obscuration techniques. We do so by attacking the redacted faces in three scenarios: obscured face identification, verification, and reconstruction. Threat modeling is also considered to provide a vulnerability analysis for each studied obscuration technique. Based on our evaluation, we show that the k-same based methods are the most effective.
Recent advances in the chemistry and biochemistry of inositol phosphates of biological interest (1990)

B. V. L. Potter, Nat. Prod. Rep., 1990, 7, 1 DOI: 10.1039/NP9900700001
Central genetic alterations common to all HCV-positive, HBV-positive and non-B, non-C hepatocellular carcinoma: A new approach to identify novel tumor markers (2006)

Hepatocellular carcinoma (HCC) is a common malignancy, but the prognosis remains poor due to the lack of sensitive diagnostic markers. To gain insight into the central molecular features common to all types of HCC, and to identify novel diagnostic markers or therapeutic targets for HCC, we performed a gene expression profiling analysis using a high throughput RT-PCR system. After examining the mRNA expression of 3,072 genes in 204 (119 tumor and 85 non-tumor) liver samples, we identified differential gene expression between the HCV group (n=80), HBV group (n=19) and non-B, non-C group (n=20) with a principal component analysis and a correlation spectrum analysis. After selection of genes differentially expressed between tumor and non-tumor tissues (p<0.01) within each HCC group, a total of 51 differentially expressed genes (23 upregulated and 28 downregulated genes) were found to be common to the three HCC groups. Gene Ontology grouping analysis revealed that genes with functions related to cell proliferation or differentiation and genes encoding extracellular proteins were found to be significantly enriched in these 51 common genes. Using an atelocollagen-based cell transfection array for functional analysis of eight upregulated genes, five (CANX, FAM34A, PVRL2, LAMR1, and GBA) significantly inhibited cellular apoptosis by two independent assays. In conclusion, we identified 51 differentially expressed genes, common to all HCC types. Among these genes, there was a high incidence of anti-apoptotic activity. This combination approach with the advanced statistical methods and the bioinformatical analysis may be useful for finding novel molecular targets for diagnosis and therapy.
[Distant metastases from carcinoma of the oral cavity and pharynx]. (1986)

Distant metastases were detected clinically in 25 (14%) of 173 patients with squamous cell carcinoma of the oral cavity and pharynx admitted from 1975 to 1982. The probability of distant metastases was higher in patients with advanced tumor (stages III and IV: 21%; T4: 27%; N3: 37%), lymphoepithelioma (57%) and in patients with a decreased lymphocyte count in the peripheral blood at presentation (less than 1300/microliter; 58%). The incidence of metastases in advanced cases was 45% in the oropharynx, 24% in the nasopharynx and 20% in the oral cavity. Whether the regional tumor had been controlled or not in advanced cases, there was no difference between the incidence and the period of detection of distant metastases.
An Integrated Broad Area Coverage Fusion Neutron/X-Ray Interrogation Unit (2007)

Homeland security has an urgent need for an advanced detecting system to accurately and quickly search for nuclear and explosive materials in a wide variety of situations. An integrated broad area coverage neutron/x-ray interrogation unit is proposed here to meet such needs. This system will use a unique cylindrical Inertial Electrostatic Confinement (IEC) device. This compact pulsed neutron/x-ray line source can produce ∼2x1010 n/s 14.1-MeV D-T neutrons, ∼108 n/s 2.45-MeV D-D neutrons and 80 kV x-rays.Unlike prior neutron activation systems, this unit can provide a long line-like emission source to obtain broad coverage, providing very fast scan time for even large objects. The use of combined multi-energy neutron and x-ray sources, along with a 3-D detector array and fuzzy logic analysis system, are expected to provide high elemental identification accuracy, greatly decreasing false signals so commonly encountered in prior systems. Analysis techniques will employ both thermal neutron analysis (TNA)and pulsed fast neutron analysis (PFNA), accompanied by broad area x-ray imaging techniques.
Nickel impurities in the Y-Ba-Cu-O 90-K superconductor (1989)

The orthorhombic superconductor ${\mathrm{YBa}}_{2}$(${\mathrm{Cu}}_{1\mathrm{\ensuremath{-}}\mathit{x}}$${\mathrm{Ni}}_{\mathit{x}}$${)}_{3}$${\mathrm{O}}_{7\mathrm{\ensuremath{-}}\mathrm{\ensuremath{\delta}}}$ with nominal values of x=0.02 and 0.05 and ${T}_{c}$=86.5 and 79.5 K, respectively, was investigated by x-ray-absorption fine-structure (XAFS) measurements about the Ni and Cu K edges. The sample was carefully characterized by various means including optical and electron microscopy. In the sample with x=0.02, the Ni was predominantly dissolved in the 1:2:3 phase. The maximum solubility found in the nominal 5% phase was x=0.04, with the excess precipitating out as NiO. The Ni atoms in the 1:2:3 phase were found to have 4.75\ifmmode\pm\else\textpm\fi{}0.4 oxygen neighbors at 1.95\ifmmode\pm\else\textpm\fi{}0.03 A\r{}. The best fit to the next neighbor was 5.1\ifmmode\pm\else\textpm\fi{}1.2 Y atoms at 2.96\ifmmode\pm\else\textpm\fi{}0.02 A\r{}, though the data are also consistent with a mixed shell of Y and Ba atoms. Although the Ni atoms have a first-neighbor environment of oxygen atoms somewhat similar to the Cu site, the more distant neighbor distances are quite different. It is concluded from both the extended and near edge XAFS that most, if not all, of the Ni atoms do not substitute in either Cu site of the 1:2:3 phase, but are in some defect site.
Multiplexing with Multi-Codeword Transmission (2010)

jp Abstract -This paper presents throughput performance results when using joint and independent modulation and coding scheme (MCS) selections in adaptive modulation and coding (AMC) for maximum likelihood detection (MLD) based signal detection in 4-by-4 Multiple-Input Multiple-Output (MIMO) multiplexing. We show by computer simulation that joint MCS selection achieves the maximum total throughput, since the best MCS combination is selected regardless of the applied MCS set. We also show that unlike for 2-by-2 MIMO multiplexing, the gain in the throughput for joint MCS selection compared to that for independent MCS selection is distinct for 4-by-4 MIMO multiplexing. Therefore, we conclude that joint MCS selection is effective in improving the maximum total throughput using MLD based signal detection for 4-by-4 MIMO multiplexing or greater.