gebhart/patrepeval huggingface dataset:
Frequency Selective Balanced SSFP Imaging with Randomised TR (2003)

The balanced SSFP signal amplitude shows strong dependency on off-resonance frequencies (banding artifact). Several methods have been proposed to broaden the stopband for fat/water selection. However, a suppression is not possible for the whole range of off-resonance frequencies. Here we present a method that accomplishes the frequency selection by randomizing TR for each sequence repetition step and acquiring several acquisitions. It offers selectivity only to the on-resonance signal and suppresses off-resonance frequencies. The method is useful for applications which require averaging, such as low gamma nuclei CSI due to low SNR.
Digital electrical impedance analysis for single bacterium sensing and antimicrobial susceptibility testing (2021)

Single-molecule and single-cell analysis techniques have opened new opportunities for characterizing and analyzing heterogeneity within biological samples. These detection methods are often referred to as digital assays because the biological sample is partitioned into many small compartments and each compartment contains a discrete number of targets (e.g. cells). Using digital assays, researchers can precisely detect and quantify individual targets, and this capability has made digital techniques the basis for many modern bioanalytical tools (including digital PCR, single cell RNA sequencing, and digital ELISA). However, digital assays are dominated by optical analysis systems that typically utilize microscopy to analyze partitioned samples. The utility of digital assays may be dramatically enhanced by implementing cost-efficient and portable electrical detection capabilities. Herein, we describe a digital electrical impedance sensing platform that enables direct multiplexed measurement of single cell bacterial cells. We outline our solutions to the challenge of multiplexing impedance sensing across many culture compartments and demonstrate the potential for rapidly differentiating antimicrobial resistant versus susceptible strains of bacteria.
Nanotechnologies pour la thérapeutique et le diagnostic (2008)

L'adressage de molécules thérapeutiques vers l'organe, le tissu, ou la cellule malade constitue aujourd'hui un défi majeur pour le traitement des maladies humaines, notamment infectieuses, cancéreuses, ou d'origine génétique. Dès le début du vingtième siècle, le savant Paul Ehrlich rêvait déjà du « magic bullet » susceptible d'acheminer un médicament de manière spécifique vers son site d'action. Le rêve de Paul Ehrlich est aujourd'hui proche de la réalité grâce au développement des nanotechnologies qui ont permis de proposer le concept de vectorisation des médicaments, dits Nanomédicaments.
A recursive system identification method based on binary measurements (2010)

An online approach to parameter estimation problems based on binary observations is presented in this paper. This recursive identification method relies on a least-mean squares approach which makes it possible to estimate the coefficients of a finite-impulse response system knowing only the system input and the sign of the system output. The impulse response is identified up to a positive multiplicative constant. The role of the regulative coefficient is investigated thanks to simulated data. The proposed method is compared with another online approach: it is shown that the proposed method is competitive with the other one in terms of estimation quality and of calculation complexity.
An Iterative Approach for local 3D-Reconstruction of Non-Rigid Movements of Heart Region From Many Angiographic Views (2003)

In this paper we examine the reconstruction of a local region of a non-rigidly moving heart with unknown motion, using methods of reconstruction of rigid objects and without using (electrocardio- gram) ECG-information. The reconstruction pro- cedure chosen is based on the well-known Feld- kamp filtered-backprojection algorithm. It recon- structs the volume of a rigid object from a reduced set of projections and their corresponding known projection matrices. The basic idea of our approach is to assume rigidity for the local region of inter- est (ROI), and to fixate it by compensating its un- known motion by a camera movement accounting for it. This is achieved using an iterative 2D-3D intensity-based registration approach.
Towards Enterprise-Ready AI Deployments Minimizing the Risk of Consuming AI Models in Business Applications (2018)

The stochastic nature of artificial intelligence (AI) models introduces risk to business applications that use AI models without careful consideration. This paper offers an approach to use AI techniques to gain insights on the usage of the AI models and control how they are deployed to a production application.
Effective re-induction therapy with dasatinib and clofarabine in relapsed Philadelphia chromosome positive acute lymphoblastic leukemia (2012)

This case discusses a 10 year old female patient with a late relapse of Ph-chromosome positive B-cell precursor acute lymphoblastic leukaemias (ALL) who had previously been treated with chemotherapy and allogeneic stem-cell transplantation. Treatment for relapse consisted of single-agent dasatinib, followed by 2 blocks of a combination of dasatinib and clofarabine as consolidation therapy. Using this schedule both morphological and cytogenetic complete remission were obtained. This regimen was well tolerated, and no major toxicity concerns occurred. Subsequently, the patient received a 2nd stem cell transplantation from a matched unrelated donor. Unfortunately, the child died after complete molecular remission at day +104 post-transplantation, due to a disseminated adenoviral infection. We conclude that dasatinib and clofarabine combination therapy was safe and effective in this patient, and should be further explored as a salvage regimen in relapsed/refractory Philadelphia chromosome positive ALL patients.
Abstract 1981: Histone demethyalase JMJD1A promotes the DNA damage response of prostate cancer cells (2016)

Abstract DNA damage is a common feature of prostate cancer (PCa) therapies (e.g. radio-, chemo- and androgen deprivation therapies). Thus, the DNA damage response (DDR) is a key factor in determining the therapeutic response of PCa patients. JMJD1A regulates gene expression by demethylating H3K9. Here, we identified a new role for JMJD1A in the DDR of PCa cells. Inhibition of JMJD1A led to increased r-H2AX foci (marker for the double strand DNA breaks) in the non-irradiated cells, and the delayed clearance of r-H2AX foci in the irradiated cells. JMJD1A knockdown in PCa cells inhibited the DNA strand break repair in the reporter assays. We further found that phosphorylation and ubiquitination were key modifications that regulate the activity of JMJD1A in the DDR. Ectopic expression of the mutant JMJD1A at the phosphorylation or ubiquitination site attenuated the expression of DNA repair factors, DNA strand break repair, and PCa cell proliferation under irradiation and androgen deprivation conditions. Our results suggest that targeting JMJD1A may sensitize the response of PCa to the major anti-PCa therapies. Citation Format: Lingling Fan, Guihong Peng, Natasha Sahgal, Ladan Fazli, Martin Gleave, Yuji Zhang, Arif Hussain, Feyruz Rassool, Jianfei Qi. Histone demethyalase JMJD1A promotes the DNA damage response of prostate cancer cells. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 1981.
In this issue: Antibodies and T Cell subtypes in diseases and therapy (2018)

Specific immunity of the mammalian host primarily consists of B and T lymphocytes that ensure specific elimination of foreign entities and establish defense memory during reinfection. Dysregulation...
Retinoid signaling alterations in amyotrophic lateral sclerosis (2008)

Amyotrophic lateral sclerosis (ALS) is characterized by the progressive degeneration and death of motor neurons (MNs) in the brain and spinal cord. Previous reports demonstrated decreased transthyretin (TTR) levels in cerebrospinal fluid of ALS patients. As TTR impacts retinoid signaling, we examined this pathway using immunohistochemistry and immunoblot. Differences in cytoplasmic proteins including cellular retinol binding protein (CRBP) and cellular retinoic acid binding proteins (CRABP)‐I and II were observed. CRBP immunoreactivity was higher in ALS spinal cord MNs while CRABP‐I was decreased in ALS. CRABP–II was concentrated to the nucleus in ALS MNs while control MNs exhibited a diffuse cytoplasmic pattern. We also assessed the expression and distribution of retinoic acid nuclear receptors. RARβ exhibited increased immunoreactivity in MN nuclei of ALS spinal cord, and RXRβ was increased in MNs of ALS spinal cord. Preliminary studies utilizing a motor neuron‐enriched culture system have evaluated the functional significance of these alterations. Our results indicate that retinoic acid signaling is altered in ALS and components of this pathway may represent novel therapeutic targets. Sources of research support: NIH grant ES013469 (RB) and NIH T32 EB001026 (CK)
DNA‐biding of IQ, Me‐IQ and Me‐IQx, strong mutagens found in broiled foods (1982)

Non-covalent DNA-binding has been studied of 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (Me-IQ) and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (Me-IQx), strong mutagens found in broiled foods. These mutagens are intercalated into DNA, as found by ultraviolet absorption and gel electrophoresis. The binding of IQ is stronger with GC pairs than AT pairs in DNA. The binding constants with calf thymus DNA are 1.6 X 10(6) (Me-IQ), 0.9 X 10(6) (IQ) and 0.7 X 10(6) M-1 (Me-IQx) at pH 6.0. This order of DNA affinity agrees with the order of mutagenicity towards Salmonella typhimurium TA98.
Characterization of the bilin attachment sites in R-phycoerythrin. (1985)

The amino acid sequence around the sites of attachment of all the bilin prosthetic groups of Gastroclonium coulteri R-phycoerythrin, (alpha beta)6 gamma, have been determined. The sequences of tryptic peptides derived from the alpha and beta subunits are (Formula: see text) where the designations alpha and beta refer to the subunits from which the peptides derived. Cysteinyl residues involved in bilin attachment are indicated with an asterisk. Each peptide carries a single bilin, either phycoerythrobilin (PEB) or phycourobilin (PUB). Spectroscopic studies on the gamma subunit indicate the presence of one PEB and three PUB groups. However, five unique tryptic peptides, gamma-A through gamma-E, were characterized, indicating that Gastroclonium R-phycoerythrin is a mixture of at least two species, (alpha beta)6 gamma and (alpha beta)6 gamma', with gamma subunits differing in amino acid sequence. The sequences of the gamma subunit bilin peptides (see below) were not homologous to those from alpha and beta subunits of any biliprotein. (Formula: see text) The bilins in all these peptides are attached through single linkages to a cysteinyl residue, except for the phycourobilin on peptide beta-3 which is attached through two thioether linkages to cysteinyl residues 10 amino acids apart. The availability of small bilin peptides was exploited to obtain more accurate molar extinction coefficients for peptide-linked PEB and PUB groups. Application of these extinction coefficients in the calculation of the bilin content of R-, B-, and C-phycoerythrins shows that there are 5 bilins/alpha beta in each of these three biliprotein types.
Projected Multi-Agent Consensus Equilibrium for Ptychographic Image Reconstruction (2021)

Ptychography is a computational imaging technique using multiple, overlapping, coherently illuminated snapshots to achieve nanometer resolution by solving a nonlinear phase-field recovery problem. Ptychography is vital for imaging of manufactured nanomaterials, but existing algorithms have computational shortcomings that limit large-scale application. In this paper, we present the Projected Multi-Agent Consensus Equilibrium (PMACE) approach for solving the ptychography inversion problem. This approach extends earlier work on MACE, which formulates an inversion problem as an equilibrium among multiple agents, each acting independently to update a full reconstruction. In PMACE, each agent acts on a portion (projection) corresponding to one of the snapshots, and these updates to projections are then combined to give an update to the full reconstruction. The resulting algorithm is easily parallelized, with convergence properties inherited from convergence results associated with MACE. We apply our method on simulated data and demonstrate that it outperforms competing algorithms in both reconstruction quality and convergence speed.
Statistical Real-Time Communication (2003)

In order to realize real-time communication over Ethernet or fast Ethernet, one must be able to bound the medium access time within an acceptable limit. The multiple access nature of Ethernet makes it impossible to guarantee a deterministic medium access time (hence, packet-delivery deadlines) to individual stations. However, one can bound the medium access time statistically by limiting the packet-arrival rate at the medium access control (MAC) layer. While considering automated manufacturing systems as the main target application, this paper addresses the connection admission control (CAC) problem for statistically bounding the medium access time of Ethernet. Specifically, a packet is guaranteed to have a medium access time smaller than a predefined bound with a certain probability if the instantaneous packet-arrival rate is kept below a certain threshold. Through a mathematical analysis, we first derived such a threshold. In order to keep the packet-arrival rate under the given threshold, we developed and installed a middleware which 1) resides between the transport layer and the Ethernet datalink layer and 2) smooths packet streams between them. The implementation of this middleware requires only a minimal change in the OS kernel without any modification to the current standard of Ethernet MAC protocol or TCP or UDP/IP stack. In order to solve the CAC problem, we derived the probability of transmitting a packet successfully upon each trial by modeling the MAC protocol, 1-persistent CSMA/CD, and the collision resolution protocol—Binary Exponential Backoff—of Ethernet. Our in-depth simulation results have shown this analytic model to provide a reasonably accurate estimate of packet-loss (or deadline-miss) ratio over Fast Ethernet. Finally, we implemented the middleware on the Linux OS, experimentally demonstrating the effectiveness of our approach in providing real-time communication over Ethernet.
The Hsa-Let-7a miRNA Enhances Ara-C Induced Apoptosis in Human Acute Myeloid Leukemia Cells (2012)

Abstract Abstract 1286 CXCR4 surface expression has been reported as an important prognostic marker in AML patients. Our group previously reported that targeting the SDF-1α/CXCR4 axis by CXCR4 inhibition could overcome the resistance of AML cells to chemotherapy both in vitro and in vivo. To further explore the mechanism of targeting CXCR4, we first performed a microRNA microarray platform and revealed that hsa-let-7a as one of the most significantly degraded microRNA in OCI-AML3 cells treated with SDF-1α. Moreover, hsa-let-7a was highly elevated when the OCI-AML3 cells were treated with CXCR4 antagonist. To investigate the role of hsa-let-7a in leukemia cells, we first attempted to transfect human hsa-let-7a mimic into the OCI-AML3 cells by electroporation. When exposed to chemotherapy agent AraC (2.5μM, 48 hours), the hsa-let-7a overexpressed cells exhibited 2 more folds of apoptosis than negative controls (Annexin V positive percentage: 69.8% ± 9.4 % vs 23.2% ± 2.7%, p&lt;0.01). Next, we suppressed CXCR4 in OCI-AML3 cells with lenti-virus delivered CXCR4 shRNA (CXCR4-shRNA-OCI3) to test whether CXCR4 regulates the hsa-let-7a expression. The CXCR4-shRNA-OCI3 cells had ∼70% lower of the cell surface CXCR4 expression as evaluated by Flow cytometry (PE-conjugated 12G5 anti-CXCR4 antibodies) and ∼60% less of migration to SDF-1α compared with the vector control cells. Using RT-PCR, we observed that hsa-let-7a level was elevated in the CXCR4-shRNA-OCI3 cells (∼2.3 folds of level in the NS-shRNA-OCI3 cells). As expected, hsa-let-7a target BCLXL were significantly decreased in CXCR4-shRNA-OCI3 cells in both mRNA and protein levels, which lead to higher sensitivity to AraC treatment (Annexin V positive percentage: 48.1% ± 5.1 % vs NS-shRNA-OCI3 cells 19.1% ± 2.7%, p&lt;0.01). To investigate whether the CXCR4 surface expression is associated with the hsa-let-7a level in patient samples, we collected bone marrow samples from AML patients after informed consent. Mononuclear cells were separated by ficoll and stained with CXCR4 antibody for cell sorting. We observed CXCR4 high expression subpopulation had significantly lower level of hsa-let-7a. c-Myc and BCLXL protein levels were much lower in CXCR4 low expression subpopulation, and it may explain that low CXCR4 expression on AML cells correlated with a better prognosis, resulting in a longer relapse-free and overall survival. Collectively, our results support that CXCR4 antagonists could mobilize AML cells from the protective stromal microenvironments and make them more susceptible to conventional therapy. These findings also showed hsa-let-7a plays an important role in AML cell chemoresistance, and suggested a new mechanism that microRNA is involved in SDF-1α/CXCR4 signaling pathway. Disclosures: No relevant conflicts of interest to declare.
Relationship of the anionic behavior of unsaturated medium-ring alcohols to structure. Generation and antarafacial cyclization of coiled 8.pi.-electron carbanions (1982)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRelationship of the anionic behavior of unsaturated medium-ring alcohols to structure. Generation and antarafacial cyclization of coiled 8.pi.-electron carbanionsLeo A. Paquette, Gary D. Crouse, and Ashok K. SharmaCite this: J. Am. Chem. Soc. 1982, 104, 16, 4411–4423Publication Date (Print):August 1, 1982Publication History Published online1 May 2002Published inissue 1 August 1982https://pubs.acs.org/doi/10.1021/ja00380a016https://doi.org/10.1021/ja00380a016research-articleACS PublicationsRequest reuse permissionsArticle Views2314Altmetric-Citations36LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
New imaging technique for 64M-DRAM (1992)

We have established a new photolithographic technique called SHRINC ( Super High Resolution by I I tumi-Nation Control ) which is based on an innovative illumination system. SHRINC improves the resolution and depth-of-focus ( DOF ) by optimum arrangement of the illumination system in respect of the angle of the Ist-order of diffraction generated by the reticle pitch. The capabilities of SHRINC have been studied by computer simulation. Results from phase shift, annular illumination, and conventional illumination are compared with those of SHRINC. The results show that using SHRINC with 0.35? m line and space patterns, the DOF, defined as the distance over which the aerial image contrast exceeds 60%, is 2. 5x larger than that obtained with conventional illumination, and almost the same as that with phase shift techniques. In our experiments we have obtained a critical resolution of 0. 275 ? m and more than 2.8 ? m DOF with 0.35? m L/S patterns, using an i-line stepper and SHRINC illumination. Moreover SHRINC is effective not only for simple line and space patterns, but also for complicated patterns with 0.30 or 0.35?m design rules, such as memory cell patterns or peripheral circuit patterns in the DRAM. From these results we conclude that i-line steppers with SHRINC will make possible mass production of 64M-DRAMs with single layer resist.
Carrier Generation in Wide‐Gap Conductor, Zinc Antimonate (2005)

Mechanisms for carrier generation in zinc antimonate (ZnSb 2 O 6 ) with a wide band gap were examined in relation to its crystallinity. ZnSb 2 O 6 prepared by the reaction of Sb 2 O 5 sol and 3ZnCO 3 ·4Zn(OH) 2 was sintered at 893, 1173, and 1393 K, and characterized by X‐ray diffraction (XRD), electrical conductivity and diffuse reflectance measurements. A higher conductivity was found for ZnSb 2 O 6 sintered at lower temperatures. Rietveld analysis of XRD patterns showed that the cations in conductive ZnSb 2 O 6 occupied irregular sites, similar to the random rutile structure. Electron carriers were also considered to be generated by oxygen vacancies in ZnSb 2 O 6 samples which have a structure similar to the random rutile structure.
A CMOS Imager for Light Blobs Detection and Processing (2005)

Biomedical optical impedance sensors involve image processing of light blobs to extract information regarding a process such as pressure. These sensors utilize a light source to illuminate an imager chip. The edges of the light blob, its diameter and location are parameters of interest to characterize pressure dynamics. CMOS technology allows the capture and processing of an image on a single chip. However, specialized imagers, optimized for extracting dynamic information from light blobs for this application, have not been proposed. A CMOS active pixel sensor (APS) imager, capable of processing all parameters of interest in a light blob, is presented. Innovative, efficient and easy-to-implement techniques for cross-sectional diameter estimation and edge detection are proposed. The simulation results showed the feasibility of the proposed design. This chip was designed using 0.18 /spl mu/m technology and is currently being fabricated. It operates at 1.8 V.
Try for Automatic on N 2-Machine. The Jumbo Rolls and Spools Transfer between Reel and Rewinder. (2002)

N2-machine for newsprint was started in Feb., 1998, at Yatsushiro mill. This machine has a lot of newest equipments, using the highest technologies. The jumbo rolls and spools handling system is one of them, and introduced into N2-machine for reasons of automatically transfer.This system has two works, the jumbo rolls are transfered by the lower guide rails from reel to rewinder, and spools are transfered by the upper guide rails from rewinder to reel. In No. 2 winder, the jumbo rolls and spools handling is carried out by Jumbo Roll Carriage which has two stages.This system has some accessory devices as follows.(1) Device for putting the rest paper on the spool into the pulper.(2) Jumbo roll paper end tape applicator.(3) Auto splice device between the jumbo rolls.Therefore automatically transfer is realized today.This paper describes about this handling system outlines and operating circumstances from startup