gebhart/patrepeval huggingface dataset:
Involvement of oligomerization, N-glycosylation and sialylation in the clearance of cholinesterases from the circulation (1995)

The possible role of post-translational modifications such as subunit oligomerization, protein glycosylation and oligosaccharide processing on the circulatory life-time of proteins was studied using recombinant human acetylcholinesterase (rHuAChE). Different preparations of rHuAChE containing various amounts of tetramers, dimers and monomers are cleared at similar rates from the circulation, suggesting that oligomerization does not play an important role in determining the rate of clearance. An engineered rHuAChE mutant containing only one N-glycosylation site was cleared from the circulation more rapidly than the wild-type triglycosylated enzyme. On the other hand, hyperglycosylated mutants containing either four or five occupied N-glycosylation sites, analagous to those present on the slowly cleared fetal bovine serum acetylcholinesterase (FBS-AChE), were also cleared more rapidly from the bloodstream than the wild-type species. Furthermore, the two different tetraglycosylated mutants were cleared at different rates while the pentaglycosylated mutant exhibited the most rapid clearance profile. These results imply that though the number of N-glycosylation sites plays a role in the circulatory life-time of the enzyme, the number of N-glycan units in itself does not determine the rate of clearance. When saturating amounts of asialofetuin were administered together with rHuAChE, the circulatory half-life of the enzyme was dramatically increased (from 80 min to 19 h) and was found to be similar to that displayed by plasma-derived cholinesterases while desialylation of these enzymes caused a sharp decrease in the circulatory half-life to approximately 3-5 min. Determination of the average number of sialic acid residues per enzyme subunit of the five different N-glycosylation species generated, revealed that the rate of clearance is not a function of the absolute number of appended sialic acid moieties but rather of the number of unoccupied sialic acid attachment sites per enzyme molecule. Specifically, we demonstrate an inverse-linear relationship between the number of vacant sialic acid attachment sites and the values of the enzyme residence time within the bloodstream.
Supplementary Figure 1 from Targeting PI3K/mTOR Overcomes Resistance to HER2-Targeted Therapy Independent of Feedback Activation of AKT (2023)

&lt;p&gt;PDF file - 772K, Figure S1: Targeting the PI3K/mTOR pathway in HER2-amplified breast cancer cell lines. Effect of A, RAD001, B, BEZ235, C, BKM120 or D, BYL719 treatment on PI3K/mTOR signaling in two representative cell lines; SKBR3 and MDA453 (PIK3CA mutant H1047R). All lysates taken 24 hours post-treatment.&lt;/p&gt;
Nonlinear interaction between signal and amplified spontaneous emission in coherent systems (2013)

We experimentally and numerically investigate the impact of nonlinear interaction between signal and ASE in coherent transmission systems. We show that dispersion management of the transmission path significantly increases penalties caused by signal-ASE interaction.
Comparison of antibody responses to different forms of HIV‐1 core antigens by epitope mapping (1997)

The specificity of antibodies to HIV-1 capsid (p24CA) and matrix (p17MA) proteins, produced in mice against unprocessed immature assembled polyprotein (wild-type p55 virus-like particles or chimeric p55 virus-like particles) or against the monomeric mature form (rp24CA/rp17MA), was analyzed by a microplate epitope mapping assay using a panel of synthetic peptides covering the entire p24CA plus p17MA sequences of HIV-1LAI. All immunized mice developed anti-p24CA and anti-p17MA antibodies, although the spectrum of specificity of these antibodies was different. Four p24 CA epitopes (residues 176–192, 201–218, 233–253, 285–304) were recognized by anti-rp24CA/rp17MA antibodies, whereas one p17MA epitope (residues 11–25) and one p24CA epitope (residues 176–192) were constantly recognized by anti-p55 virus-like particle antibodies. These results suggest a different specificity pattern of anti-p24CA and anti-p17MA antibodies depending on whether they are produced against the soluble mature form or the immature assembled form of the gag proteins. J. Med. Virol. 51:145–151, 1997. © 1997 Wiley-Liss, Inc.
Low threshold (GaIn)(NAs) semiconductor disk laser emitting at 1260nm (2007)

We demonstrate a 1260 nm GalnNAs semiconductor disk laser grown by MOVPE showing threshold densities as low as l.lkW/cm2 and slope efficiencies of almost 13% at room temperature while pumping at 808 nm.
Intravascular Gas Transfer (1997)

Single and double hollow fiber intravascular gas exchangers were evaluated in an extracorporeal veno-venous bypass circuit (right atrium to pulmonary artery) including a tubular blood chamber (mimicking caval veins with an inner diameter of 26 mm) for evaluation of the membrane surface area/host vessel diameter gas transfer relationships. Six bovine experiments (body wt: 68 ± 4 kg) with staged ex vivo blood flows of 1, 2, 3, and 4 L/min and a device oxygen inflow of 0, 3, and 6 L/min (0 or 3 L/min/device) were performed. Total oxygen transfer at a blood flow of 1 L/min was 33 ± 4 ml/min for a gas flow of 3 L/min (one device) vs 60 ± 25 ml/min for a gas flow of 6 L/min (two devices); at a blood flow of 2 L/min, the corresponding oxygen transfer was 46 ± 16 ml/min for a gas flow of 3 L/min vs 95 ± 44 ml/min for a gas flow of 6 L/min; at a blood flow of 3 L/min, the corresponding oxygen transfer was 48 ± 24 ml/min for a gas flow of 3 L/min vs 92 ± 37 ml/min for a gas flow of 6 L/min (p < 0.01 for comparison of areas under the curves). Total carbon dioxide transfer at a blood flow of 1 L/min was 47 ± 18 ml/min for a gas flow of 3 L/min vs 104 ± 26 ml/min for a gas flow of 6 L/min; at a blood flow of 2 L/min, the corresponding carbon dioxide transfer was 59 ± 19 ml/min for a gas flow of 3 L/min vs 129 ± 39 ml/min for a gas flow of 6 L/min; at a blood flow of 3 L/min, the corresponding carbon dioxide transfer was 60 ± 22 ml/min for a gas flow of 3 L/min vs 116 ± 49 ml/min for a gas flow of 6 L/min (p < 0.01). For the given setup, the blood flow/gas transfer relationship is non linear, and a plateau is achieved at a blood flow of 2.5 L/min for O2 and CO2. Doubling membrane surface area and consecutively sweeping gas flows result in doubling of gas transfers at all tested blood flows. However, increased membrane surface area and blood flow produce a higher pressure drop that in turn limits the fiber density that can be used clinically.
Civic ceremony in early modern London (1986)

The ceremonial life of the early modern town has emerged as an important area of study for urban historians. Ever since the publication of Charles Phythian-Adams' innovative study, attention has focused on the elaborate series of processions, pageants and rites of passage which were a constant feature of the yearly cycle of town life. In the wake of his work other studies of the ceremonial life of late medieval and early modern towns have added to the urban historian's awareness of the importance of ritualized forms of behaviour as a symbolic thread which helped bind together the social fabric of the townscape. The research done thus far forms one of those areas of scholarship in which the cross-fertilization of the interdisciplinary approach, so beloved of the ‘new’ urban history, has proved particularly fruitful. It might be surprising then that London, the largest of English towns, has yet to receive similar treatment. There have been, of course, several important studies of the capital in the early modern period, and they have something to say about the ceremonial life of the city. These have largely concentrated on topics such as the structure of government, the nature of London's ruling elite, and social mobility within the city's craft organizations. While providing important sidelights on ceremony, these treatments have tended to place ceremonial events in the background, as the ‘icing on the cake’. At the same time there have been many important studies by art and literary historians of ceremonial occasions such as coronations, royal entries or the Lord Mayors' Shows, but these have tended to concentrate on ceremony as an expression of dynastic propaganda or as a development in dramatic form rather than as part of the social history of the city. The object of this article will be to attempt to rectify this gap in our knowledge by applying some of the framework bequeathed by Phythian-Adams, and so try to assess the relevence of his conclusions about urban society in this period to the particular case of London.
IMPACTS OF BARRIER INSECTICIDE MIXTURES ON MOSQUITO, AEDES AEGYPTI AND NON-TARGET HONEY BEE, APIS MELLIFERA (2022)

Four novel commercial insecticide mixtures, composed of pyrethroid and nicotinoid active ingredients, were evaluated in a series of experiments in the laboratory, semi-field and field to determine acute toxicity (LC50) against pyrethroid-susceptible (ORL1952) and resistant (Puerto Rico) strains of Aedes aegypti L., and non-target adult European honey bees, Apis mellifera L. The four products were Tandem, Temprid FX, Transport Mikron, and Crossfire. The acute toxicity data showed that pyrethroid-resistant Ae. aegypti PR exhibited decreased sensitivity to all 4 insecticide mixtures, compared to pyrethroid-susceptible Ae. aegypti ORL1952. Tandem, Temprid FX, and Transport Mikron were more toxic to Ae. aegypti ORL1952 than to A. mellifera, but Crossfire was the least toxic. Transport Mikron was also more toxic to Ae. aegypti PR than to A. mellifera. The Honey bee Tolerance Indexes, determined with LC50 data of pyrethroid-susceptible mosquitoes, demonstrated that while Transport Mikron, Tandem, and Temprid FX were more toxic to Ae. aegypti ORL1952 than to A. mellifera, Crossfire was less toxic. The honey bee Tolerance Indexes decreased substantially when calculated with LC50 data from pyrethroid- resistant mosquitoes, but honey bees remained tolerant of Transport Mikron. Notably, while the insecticide mixtures did not control the PR resistant Ae. aegypti strain when applied as residual sprays to perimeter vegetation at label rates, susceptible Ae. aegypti ORL1951 were controlled, but applications affected honeybees (A. mellifera) for up to 28 days after treatment. Temprid FX resulted in 74% and 99% mortality, in adult Ae. aegypti ORL1952 and A. mellifera, respectively, for 28 days post-treatment. Transport Mikron and Tandem residues killed Ae. aegypti ORL1952 for up to 21 days post-treatment, while the effect of Crossfire lasted only 14 days. All three insecticides killed A. mellifera for up to 28 days post-treatment but at decreased mortality rates. For operational mosquito control, these data indicate that Transport Mikron has a reasonable safety margin (~25%) when targeting susceptible mosquitoes, compared to Tandem, Temprid FX, and Crossfire. The tested insecticide formulations need to be applied in higher doses to control resistant strains of mosquitoes that may be detrimental to honey bees. The ULV data indicated that pyrethroid resistance can be overcome with the insecticide mixtures.
A direct-detection LIDAR detector for the Europa lander concept (2022)

Jet Propulsion Laboratory is developing a Europa Lander astrobiology mission concept to search for biosignatures within Europa’s subsurface. However, Europa’s rugged terrain presents a number of physical hazards for landing. MIT Lincoln Laboratory is designing a radiation-hardened real-time direct-detection LIDAR system at 532nm to aid with autonomous hazard avoidance and landing site selection for this Europa Lander concept. The detector for this system is a 2048x32 array of silicon Geiger-mode APDs and covers the required field-of-view in one dimension, removing the need for 2D stitching and enabling real-time data processing. Detector design, improvements for radiation tolerance and component characterization results are presented.
ChemInform Abstract: Immobilization of Olefin Metathesis Catalysts on Monolithic Sol—Gel: Practical, Efficient and Easily Recyclable Catalysts for Organic and Combinatorial Synthesis. (2002)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Distance dependence of path loss models with weighted fitting (2016)

The path loss model describing the power-law dependency on distance plus a log-normally distributed shadowing attenuation, is a staple of link budgets and system simulations. Determination of the parameters of this model is usually done from measurements and ray tracing. We show that the typical least-square fitting to those data points is inherently biased to give the best fitting to the link distances that happen to have more evaluation points; this bias might be highly undesirable in various types of simulations that use the resulting model. In this paper we present a weighted fitting method to address this issue. While it is unavoidable that fits are better for one distance range than another, we argue that such a decision should be made consciously, and adjusted to the type of simulation for which the path loss model should be used. We discuss the weighting functions for different purposes, and show their impact on prediction accuracy of signal level, interference level, and capacity in a hexagonal cellular grid simulation. As examples, weighted fitting models are presented for 28 GHz channels in urban macrocells, and it is shown that the fitting accuracy can be improved by our approach.
Extraction and transport of alkali metal salts by crown ethers and cryptands: estimation of extraction constants and their relationship to transport flux (1987)

A method is described for the estimation of two phase extraction constants from stability constants for complexation of alkali metal cations by crown ethers and cryptands. The total free energy change for extraction of alkali metal salts from water to a non-polar organic solvent is evaluated as a sum of free energy terms for complexation, transfers of cation, anion, and complex between solvents, and ion pairing in the non-polar solvent. The method gives satisfactory agreement with a variety of published extraction constants and can be used predictively. As an example, the influence of extraction constant on transport flux was examined using calculated extraction constants and the rate data of Lamb etal. (J. Am. Chem. Soc. 102, 6820 (1980)); rates are adequately modelled by a simple analysis of diffusion.
Hydrogen passivation of laser-induced defects for silicon solar cells (2014)

Hydrogen passivation of laser-induced defects is shown to be essential for the fabrication of laser doped solar cells. On first generation laser doped selective emitter solar cells where open circuit voltages are predominately limited by the full area back surface field, a 10 mV increase and 0.4 % increase in pseudo fill factor is observed through hydrogen passivation of defects generated during the laser doping process resulting in an efficiency gain of 0.35 % absolute. The passivation of such defects becomes of increasing importance when developing higher voltage devices, and can result in improvements on test structures up to 25 mV. On n-type PERT solar cells, an efficiency gain of 0.7 % absolute is demonstrated with increases in open circuit voltage and pseudo fill factor by applying a short low temperature hydrogenation process incorporating minority carrier injection using only hydrogen within the device. This process is also shown to improve the rear surface passivation increasing the short circuit current density from long wavelengths 0.2 mA/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> compared to that achieved using an Alneal process. Subsequently an average efficiency of 20.54 % is achieved.
Usure du polyéthylène à 10 ans dans l'arthroplastie totale de hanche : influence de l'offset fémoral (2008)

L'usure du polyethylene reste un des principaux problemes, dans la duree de vie d'une prothese totale de hanche. Le propos de l'etude est d'evaluer, a long terme, l'usure du polyethylene et de correler une majoration ou une minoration d'usure, a la valeur de l'offset femoral. Le positionnement des implants a ete mesure par logiciel informatique, sur une population homogene de 66 arthroplasties totales de hanche pour coxarthrose ou osteonecrose, apres numerisation des cliches radiographiques, en comparant trois cliches (bassin de face preoperatoire, postoperatoire immediat et postoperatoire a 10 ans). La methode EBRA permet d'assurer la reproductibilite et la comparaison des differents cliches. Le couple de frottement de cette etude est represente par l'alumine et le polyethylene. L ' inclinaison, le positionnement horizontal et vertical du cotyle, l'offset femoral et l'usure du polyethylene ont ete mesures a 10 ans de recul. Cette usure est mesuree grace aux differences de positionnement entre le centre de tete femoral et le centre cotyloidien. Le taux d'usure lineaire annuel est de 0.11 mm/annee. Les resultats montrent que, meilleure est la restitution de l'offset femoral par la tige, moindre est l'usure du polyethylene. Une correlation, par analyse de regression lineraire simple, est mise en evidence entre l'usure et le pourcentage de lateralisation femorale restituee. Aucune relation n'a pu etre mise en evidence entre l'usure du polyethylene et les parametres positionnels du cotyle.
N-Heterocyclic 3-Pyridyl Carboxamide Inhibitors of DHODH for the Treatment of Acute Myelogenous Leukemia (2022)

Acute myelogenous leukemia (AML), a disease of the blood and bone marrow, is characterized by the inability of myeloblasts to differentiate into mature cell types. Dihydroorotate dehydrogenase (DHODH) is an enzyme well-known in the pyrimidine biosynthesis pathway; however, small molecule DHODH inhibitors were recently shown to induce differentiation in multiple AML subtypes. Using virtual screening and structure-based drug design approaches, a new series of N-heterocyclic 3-pyridyl carboxamide DHODH inhibitors were discovered. Two lead compounds, <b>19</b> and <b>29</b>, have potent biochemical and cellular DHODH activity, favorable physicochemical properties, and efficacy in a preclinical model of AML.
Benzylic Grignard reagents: application of [Mg(anthracene)(thf)3](thf = tetrahydrofuran) in regioselective Grignard formation and C–O cleavage in benzyl ethers (1988)

Benzylic Grignard reagents (2)–(4), bearing ortho- and para-halogeno ring substituents, are readily accessible by treating the corresponding benzylic halide with [Mg(anthracene)(thf)3](1) in tetrahydrofuran (thf); o- and p-chloromethyl(methoxymethyl)benzenes with (1) rapidly yield ‘di-Grignards’ whereas the meta-isomer only affords a mono-Grignard' (5), and bis(methoxymethyl)benzenes slowly undergo C–O cleavage, (6).
Absence of Oxalobacter Formigenes in Cystic Fibrosis Patients: A Risk Factor for Hyperoxaluria (1999)

No AccessJournal of UrologyUrological Survey: Abstracts: Urolithiasis, Endourology and Laparoscopic Surgery1 May 1999Absence of Oxalobacter Formigenes in Cystic Fibrosis Patients: A Risk Factor for Hyperoxaluria H. Sidhu, B. Hoppe, A. Hesse, K. Tenbrock, S. Bromme, E. Rietschel, and A.B. Peck H. SidhuH. Sidhu , B. HoppeB. Hoppe , A. HesseA. Hesse , K. TenbrockK. Tenbrock , S. BrommeS. Bromme , E. RietschelE. Rietschel , and A.B. PeckA.B. Peck View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)69046-5AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "Absence of Oxalobacter Formigenes in Cystic Fibrosis Patients: A Risk Factor for Hyperoxaluria." The Journal of Urology, 161(5), p. 1733 Division of Oxalate Research, Ixion Biotechnology, Alachua, Florida© 1999 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 161Issue 5May 1999Page: 1733 Advertisement Copyright & Permissions© 1999 by American Urological Association, Inc.MetricsAuthor Information H. Sidhu More articles by this author B. Hoppe More articles by this author A. Hesse More articles by this author K. Tenbrock More articles by this author S. Bromme More articles by this author E. Rietschel More articles by this author A.B. Peck More articles by this author Expand All Advertisement PDF downloadLoading ...
Different Evolution of Genotypic Resistance Profiles to Emtricitabine Versus Lamivudine in Tenofovir-Containing Regimens (2010)

Our study shows lower rates of M184V development in FTC + TDF regimens versus 3TC + TDF and suggests a potential role of boosted protease inhibitors and TDF in delaying the M184V emergence. Novel RT mutational patterns, more complex than currently known, can contribute to 3TC, FTC, and TDF resistance.
Broadband Liquid Crystal Tunable Metasurfaces in the Visible: Liquid Crystal Inhomogeneities Across the Metasurface Parameter Space (2021)

Optical metasurfaces—planar nanostructured devices that can arbitrarily tailor the wavefront of light—may be reconfigured by changing their dielectric environment. The application of external stimuli to liquid crystals is a particularly promising means of tuning the optical properties of embedded metasurfaces because of liquid crystals’ large and broadband optical anisotropy. However, the detailed behavior of liquid crystals immediately adjacent to the nanostructured meta-atoms elements is often overlooked, despite the optics of the device depending sensitively on this behavior (e.g., the spectral position of the meta-atom resonances). This is of increasing concern as the wavelength of operation further approaches the short-wavelength end of the visible spectrum and, therefore, the length scale of the inhomogeneities in the liquid crystal director field. In this manuscript, we undertake a fully comprehensive study, across the metasurface geometrical parameter space, of broadband (450–700 nm) all-dielectric liquid crystal tunable metasurfaces operating in the visible. Through combined experimental characterization, liquid crystal modeling, and optical simulations, we reveal and quantify the improved accuracy with which the optical properties of the liquid crystal tunable metasurfaces may be described, and identify the underlying physical mechanism: the three-dimensional spatial overlap of the liquid crystal director field and metasurface optical near fields in the vicinity of the meta-atoms.