gebhart/patrepeval huggingface dataset:
Molecular structures of trimeric HIV-1 Env in complex with small antibody derivatives (2012)

The extensive carbohydrate coat, the variability of protein structural features on HIV-1 envelope glycoproteins (Env), and the steric constraints of the virus-cell interface during infection, present challenges to the elicitation of effective full-length (~150 kDa), neutralizing antibodies against HIV. These hurdles have motivated the engineering of smaller antibody derivatives that can bind Env and neutralize the virus. To further understand the mechanisms by which these proteins neutralize HIV-1, we carried out cryoelectron tomography of native HIV-1 BaL virions complexed separately to two small (~15 kDa) HIV-neutralizing proteins: A12, which binds the CD4-binding site on Env, and m36, whose binding to Env is enhanced by CD4 binding. We show that despite their small size, the presence of these proteins and their effects on the quaternary conformation of trimeric Env can be visualized in molecular structures derived by cryoelectron tomography combined with subvolume averaging. Binding of Env to A12 results in a conformational change that is comparable to changes observed upon its binding to the CD4-binding site antibody, b12. In contrast, binding of Env to m36 results in an "open" quaternary conformation similar to that seen with binding of soluble CD4 or the CD4i antibody, 17b. Because these small neutralizing proteins are less sterically hindered than full-length antibodies at zones of virus-cell contact, the finding that their binding has the same structural consequences as that of other broadly neutralizing antibodies highlights their potential for use in therapeutic applications.
Ring‐Closing Olefin Metathesis for the Synthesis of Benzene Derivatives. (2007)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Transcriptional profiling unveils type I and II interferon networks in blood and tissues across diseases (2019)

Understanding how immune challenges elicit different responses is critical for diagnosing and deciphering immune regulation. Using a modular strategy to interpret the complex transcriptional host response in mouse models of infection and inflammation, we show a breadth of immune responses in the lung. Lung immune signatures are dominated by either IFN-γ and IFN-inducible, IL-17-induced neutrophil- or allergy-associated gene expression. Type I IFN and IFN-γ-inducible, but not IL-17- or allergy-associated signatures, are preserved in the blood. While IL-17-associated genes identified in lung are detected in blood, the allergy signature is only detectable in blood CD4<sup>+</sup> effector cells. Type I IFN-inducible genes are abrogated in the absence of IFN-γ signaling and decrease in the absence of IFNAR signaling, both independently contributing to the regulation of granulocyte responses and pathology during Toxoplasma gondii infection. Our framework provides an ideal tool for comparative analyses of transcriptional signatures contributing to protection or pathogenesis in disease.
Measurement of Forces between Spontaneous Vesicle-Forming Bilayers (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMeasurement of Forces between Spontaneous Vesicle-Forming BilayersS. Chiruvolu, J. N. Israelachvili, E. Naranjo, Z. Xu, J. A. Zasadzinski, E. W. Kaler, and K. L. HerringtonCite this: Langmuir 1995, 11, 11, 4256–4266Publication Date (Print):November 1, 1995Publication History Published online1 May 2002Published inissue 1 November 1995https://pubs.acs.org/doi/10.1021/la00011a016https://doi.org/10.1021/la00011a016research-articleACS PublicationsRequest reuse permissionsArticle Views370Altmetric-Citations50LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A 20 Gb/s InP DHBT driver IC with high output voltage swing for direct and external laser modulation (2002)

A 20 Gb/s driver IC developed for direct and external laser modulation is presented. The single-ended output voltage swing is as high as 4 V/sub pp/ (8 V/sub pp/ differential) at 50 /spl Omega/ load. The circuit was fabricated in a standard InP-InGaAs double heterojunction bipolar transistor technology.
Printable phosphorescent organic light‐emitting devices (2009)

A new approach to full‐color printable phosphorescent organic light‐emitting devices (P 2 OLEDs) is reported. Unlike conventional solution‐processed OLEDs that contain conjugated polymers in the emissive layer, the P 2 OLED's emissive layer consists of small‐molecule materials. A red P 2 OLED that exhibits a luminous efficiency of 11.6 cd/A and a projected lifetime of 100,000 hours from an initial luminance of 500 cd/m 2 , a green P 2 OLED with a luminous efficiency of 34 cd/A and a projected lifetime of 63,000 hours from an initial luminance of 1000 cd/m 2 , a light‐blue P 2 OLED with a luminous efficiency of 19 cd/A and a projected lifetime 6000 hours from an initial luminance of 500 cd/m 2 , and a blue P 2 OLED with a luminous efficiency of 6.2 cd/A and a projected lifetime of 1000 hours from an initial luminance of 500 cd/m 2 is presented.
Hearing loss: The final frontier of pharmacology (2022)

Despite a prevalence greater than cancer or diabetes, there are no currently approved drugs for the treatment of hearing loss. Research over the past two decades has led to a vastly improved understanding of the cellular and molecular mechanisms in the cochlea that lead to hearing deficits and the advent of novel strategies to combat them. Combined with innovative methods that enable local drug delivery to the inner ear, these insights have paved the way for promising therapies that are now under clinical investigation. In this review, we will outline this renaissance of cochlear biology and drug development, focusing on noise, age-related, and chemotherapy-induced hearing dysfunction.
Enhanced autoimmune arthritis in IFN-gamma receptor-deficient mice is conditioned by mycobacteria in Freund's adjuvant and by increased expansion of Mac-1+ myeloid cells. (1999)

Induction of experimental autoimmune diseases often relies on immunization with the organ-specific autoantigens in CFA, which contains heat-killed mycobacteria. In several of these models, including collagen-induced arthritis, endogenous IFN-gamma acts as a disease-limiting factor in the pathogenesis of the disease. Here we show that in collagen-induced arthritis the protective effect of IFN-gamma depends on the presence of mycobacteria in the adjuvant. Omission of mycobacteria inverts the role of endogenous IFN-gamma to a disease-promoting factor. Thus, the mycobacterial component of CFA opens a pathway by which endogenous IFN-gamma exerts a protective effect that supersedes its otherwise disease-promoting effect. Extramedullary hemopoiesis and expansion of the Mac-1+ cell population accompanied the accelerated and more severe disease course in the IFN-gamma receptor knockout mice immunized with CFA. Treatment of such mice with Abs against the myelopoietic cytokines IL-6 or IL-12 inhibited both disease development and the expansion of the Mac-1+ population. We postulate that mycobacteria in CFA stimulate the expansion of the Mac-1+ cell population by a hemopoietic process that is restrained by endogenous IFN-gamma. These results have important implications for the validity of animal models of autoimmunity to study the pathogenesis and to evaluate cytokine-based therapy of autoimmune diseases.
Pharmacological approaches to the treatment of COVID-19 patients (2020)

The current COVID-19 pandemic has presented unprecedented challenges to the world community. No effective therapies or vaccines have yet been established. Upon the basis of homologies to similar coronaviruses, several potential drug targets have been identified and are the focus of both laboratory and clinical investigation. The rationale for several of these drug candidates is presented in this review. Emerging clinical data has revealed that severe COVID-19 disease is associated with heightened inflammatory responses and a procoagulant state, suggesting that patient treatment strategies must extend beyond antiviral agents. Effective approaches to the treatment of vulnerable patients with comorbidities will render COVID-19 substantially more manageable.
Everybody needs somebody: Modeling social and grouping behavior on a linear programming multiple people tracker (2011)

Multiple people tracking consists in detecting the subjects at each frame and matching these detections to obtain full trajectories. In semi-crowded environments, pedestrians often occlude each other, making tracking a challenging task. Most tracking methods make the assumption that each pedestrian's motion is independent, thereby ignoring the complex and important interaction between subjects. In this paper, we present an approach which includes the interaction between pedestrians in two ways: first, considering social and grouping behavior, and second, using a global optimization scheme to solve the data association problem. Results on three challenging publicly available datasets show our method outperforms state-of-the-art tracking systems.
Thermodynamic modeling of concentrated aqueous electrolyte and nonelectrolyte solutions (1995)

Abstract A new approach is presented for calculation of activity coefficients in aqueous electrolyte solutions. This approach requires only standard thermodynamic properties for the pure components and for the solutes at infinite dilution. No adjustable mixture parameters are necessary. This new procedure has been applied to calculate: (1) the solubility of salts and the activity of water in binary aqueous solutions; (2) the temperature dependence of the solubility of salts; (3) the azeotropic composition of a system containing a volatile electrolyte, (H 2 HNO 3 ); (4) the solubility of carbon dioxide in water at elevated pressures and at different temperatures.
Optimización del diagnóstico de hepatitis por citomegalovirus en receptores de trasplante hepático: diez años de experiencia (2020)

Introduccin: Para los pacientes receptores de trasplante heptico (TH) la hepatitis por citomegalovirus (CMV) constituye una entidad de difcil diagnstico. Nuestro objetivo fue determinar la real incidencia de hepatitis por CMV aplicando tcnicas diagnsticas ms especficas. Material y Mtodos: Estudio retrospectivo/ prospectivo, en un centro de trasplante heptico. Perodo de estudio:
Use of the surface proteins GapC and Mig ofStreptococcus dysgalactiaeas potential protective antigens against bovine mastitis (2004)

Streptococcus dysgalactiae is a significant pathogen associated with bovine mastitis in lactating and nonlactating dairy cows, causing a severe inflammatory response of the mammary gland, which results in major economic losses to the dairy industry. Two proteins from S. dysgalactiae strain SDG8 were tested for their protective capacity against a homologous bacterial challenge in a dry cow model. The first was a bovine plasmin receptor protein (GapC), which shares 99.4% sequence identity to the plasmin-binding Plr protein of group A streptococci. The second protein product was Mig, a alpha2-M-, IgG-, and IgA-binding protein present on the cell surface of SDG8. We investigated the efficacy of immunization with purified recombinant forms of GapC and Mig by measuring the number of somatic cells and assessing the presence of the challenge strain in mammary secretions following challenge. In this model, we found that, although the number of quarters containing SDG8 was significantly reduced in the GapC- but not in the Mig-immunized animals, the somatic cell counts from teat secretions were significantly decreased in both the GapC and Mig vaccinates.
Development trend of material and manufacturing process for fossil power generation (2016)

This paper presents an overview of worldwide electric power development and National <TEX>$700^{\circ}C$</TEX> Hyper Supercritical coal-fired power generation(HSC) focus on materials and manufacturing process. To Increase the efficiency of electric power generation, It is necessary to increase steam temperature and pressure. In that case, New material and manufacturing process shall be developed for boiler and turbine component in high temperature and pressure operating condition. Therefore, Much Efforts in worldwide are progressing to develop materials and manufacturing technology and to build and operate an HSC.
[Transforming growth factor-beta (TGF-beta) inhibits epithelial wound healing of organ-cultured rabbit cornea]. (1993)

Transforming growth factor-beta (TGF-beta) is a unique polypeptide growth factor which has been found to stimulate mesenchymal cell proliferation and inhibit epithelial cell growth. Whether or not TGF-beta inhibits corneal epithelial wound closure remains controversial. This study evaluated the effects of TGF-beta on corneal epithelial wound healing in vitro. Rabbit corneas with an artificial 8 mm epithelial defect were organ-cultured for 48 hr in TC-199 supplemented with TGF-beta (100 pg/ml, 1 ng/ml, or 10 ng/ml). The contralateral eye served as control. TGF-beta at 1 ng/ml and 10 ng/ml concentrations showed significant decrease in wound-healing rate compared with the control. 5-bromo-2'-deoxyuridine-immunohistochemistry showed a significant decrease in S-phase cell count in the 1 ng/ml group compared with the control. These results strongly suggest that TGF-beta inhibits corneal epithelial wound healing.
NEPTUNE regional observatory system design (2002)

A team from the University of Washington (UW), the Woods Hole Oceanographic Institute (WHOI), the Monterey Bay Aquarium Research Institute (MBARI), the Jet Propulsion Laboratory (JPL), and the Institute for Pacific Ocean Science and Technology (IPOST) of Canada is jointly developing the North East Pacific Time-series Underwater Networked Experiment (NEPTUNE). NEPTUNE is being designed as a long-lived, highly reliable underwater observatory. Its basic intent is to instrument the Juan de Fuca tectonic plate, which lies just off the coasts of Washington, Oregon, and British Columbia. NEPTUNE will use standard telecommunication fiber optic cable to provide a communication and power infrastructure. A high-speed data link (up to 1 Gb/second) and high power (at least 5 kW/node) will be available at 30 nodes spaced at approximately 100-km intervals over the tectonic plate. Each node will act as a local relay point for scientific instruments and experiments that will be deployed on the sea floor and in the water column above it. The installation of such instruments will be on a "plug and play" basis, which will allow for expansion of capability as new instruments are developed and instruments that need repair or have completed their experiments are removed. The scientific investigations enabled by the NEPTUNE observatory are very diverse. This paper presents the current design of the observatory, including descriptions of the key subsystems. Innovative approaches are being developed to provide high power, broadband communications, data archiving, and accurate timing and control. The methodology for ensuring the reliability and affordability of the observatory is discussed.
Trajectory of microparticles actuated with standing surface acoustic waves in microfluidic devices (2015)

This article deals with the development of a two-dimensional dynamic model for predicting the trajectory of microparticles in an acoustic field, associated with standing surface acoustic wave, on a continuous flow microfluidic device. The model consists of two governing equations, each describing the motion of the microparticle. The model is solved using finite difference method; the solution provides the displacements of the microparticles, in the two directions, for the time duration of interest. The model is subsequently employed for parametric study. The parameters considered include the width of the microchannel, radius of microparticles, initial transverse displacement of the microparticle and volumetric flow rate. The primary application of this model article would be in the design process.
Zero-shifting Technique for Deep Neural Network Training on Resistive Cross-point Arrays (2019)

A resistive memory device-based computing architecture is one of the promising platforms for energy-efficient Deep Neural Network (DNN) training accelerators. The key technical challenge in realizing such accelerators is to accumulate the gradient information without a bias. Unlike the digital numbers in software which can be assigned and accessed with desired accuracy, numbers stored in resistive memory devices can only be manipulated following the physics of the device, which can significantly limit the training performance. Therefore, additional techniques and algorithm-level remedies are required to achieve the best possible performance in resistive memory device-based accelerators. In this paper, we analyze asymmetric conductance modulation characteristics in RRAM by Soft-bound synapse model and present an in-depth analysis on the relationship between device characteristics and DNN model accuracy using a 3-layer DNN trained on the MNIST dataset. We show that the imbalance between up and down update leads to a poor network performance. We introduce a concept of symmetry point and propose a zero-shifting technique which can compensate imbalance by programming the reference device and changing the zero value point of the weight. By using this zero-shifting method, we show that network performance dramatically improves for imbalanced synapse devices.
Multiple-layer optical interconnections using through-wafer hollow-dielectric-waveguide vias (1994)

We investigate lossy hollow-dielectric-waveguide vias in a dense optical interconnection system as a means by which many 2-D processor layers can communicate simultaneously with minimal signal attenuation. Via-guided multiple-layer signal attenuation and design guidelines are derived for both GaAs and Si substrate materials, and are compared to those for unguided interconnections at 0.85 μm and 1.3 μm. We find that optical interconnections using hollow-dielectric-waveguide vias can support dense low-loss interconnections at 0.85 μm, whereas propagation through the substrate is preferred at 1.3 μm.
In Vivo Application and Imaging of Intralenticular Femtosecond Laser Pulses for the Restoration of Accommodation (2008)

It is possible to create microincisions inside the crystalline lens within an acceptably short treatment time (<30 seconds). The 14-day follow-up did not show undesirable side effects, such as cataract formation, after intralenticular laser treatment.
Surgical treatment of zygomatic bone fracture using two points fixation versus three point fixation-a randomised prospective clinical trial (2012)

Based on this study open reduction and internal fixation using three point fixation by miniplates is the best available method for the treatment zygomatic bone fractures.
Species-specific host factors rather than virus-intrinsic virulence determine primate lentiviral pathogenicity (2018)

HIV-1 causes chronic inflammation and AIDS in humans, whereas related simian immunodeficiency viruses (SIVs) replicate efficiently in their natural hosts without causing disease. It is currently unknown to what extent virus-specific properties are responsible for these different clinical outcomes. Here, we incorporate two putative HIV-1 virulence determinants, i.e., a Vpu protein that antagonizes tetherin and blocks NF-κB activation and a Nef protein that fails to suppress T cell activation via downmodulation of CD3, into a non-pathogenic SIVagm strain and test their impact on viral replication and pathogenicity in African green monkeys. Despite sustained high-level viremia over more than 4 years, moderately increased immune activation and transcriptional signatures of inflammation, the HIV-1-like SIVagm does not cause immunodeficiency or any other disease. These data indicate that species-specific host factors rather than intrinsic viral virulence factors determine the pathogenicity of primate lentiviruses.
Palladium-Mediated Benzylation of Dimetalated 1-Butylindole-3-carboxylic Acid (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPalladium-Mediated Benzylation of Dimetalated 1-Butylindole-3-carboxylic AcidLawrence E. Fisher, Sharada S. Labadie, Deborah C. Reuter, and Robin D. ClarkCite this: J. Org. Chem. 1995, 60, 19, 6224–6225Publication Date (Print):September 1, 1995Publication History Published online1 May 2002Published inissue 1 September 1995https://pubs.acs.org/doi/10.1021/jo00124a050https://doi.org/10.1021/jo00124a050research-articleACS PublicationsRequest reuse permissionsArticle Views166Altmetric-Citations17LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Development of an Anti-Idiotype Aptamer-Based Electrochemical Sensor for a Humanized Therapeutic Antibody Monitoring (2023)

Therapeutic monoclonal antibodies (mAbs) are currently the most effective medicines for a wide range of diseases. Therefore, it is expected that easy and rapid measurement of mAbs will be required to improve their efficacy. Here, we report an anti-idiotype aptamer-based electrochemical sensor for a humanized therapeutic antibody, bevacizumab, based on square wave voltammetry (SWV). With this measurement procedure, we were able to monitor the target mAb within 30 min by employing the anti-idiotype bivalent aptamer modified with a redox probe. A fabricated bevacizumab sensor achieved detection of bevacizumab from 1–100 nM while eliminating the need for free redox probes in the solution. The feasibility of monitoring biological samples was also demonstrated by detecting bevacizumab in the diluted artificial serum, and the fabricated sensor succeeded in detecting the target covering the physiologically relevant concentration range of bevacizumab. Our sensor contributes to ongoing efforts towards therapeutic mAbs monitoring by investigating their pharmacokinetics and improving their treatment efficacy.