gebhart/patrepeval huggingface dataset:
Abstract 248: Role of Mitochondrial Heat-Shock Protein 90 (HSP90) in Regulation of Vascular Smooth Muscle Cell (VSMC) Apoptosis and Vascular Remodeling in-vivo (2013)

Introduction Apoptosis of VSMC is a common feature of vascular remodeling. Mitochondrial apoptosis is regulated by an organelle-specific network including Hsp90 family proteins. Gamitrinib (G4) is a small molecule that selectively targets mitochondrial Hsp90 and has shown antitumor activity. We investigated the effects of G4 on mitochondrial membrane potential (MMP) and apoptosis in VSMC, and on vascular remodeling in vivo. Methods Primary cultured human VSMCs were treated with G4, 17-AAG (inhibits cytosolic Hsp90), or vehicle. MMP (TMRM assay), apoptosis (Annexin V/PI flow cytometry) and cell viability (MTT assay) were measured. For in vivo studies, a mouse carotid artery ligation model was used. Mice (N= 45) were treated with systemic G4 (10 mg/kg IP daily) vs. control, and sacrificed at 3 or 14 days. Results Pre-treatment of VSMC with G4 (1 uM) resulted in a rapid, dose-dependent loss of MMP upon exposure to peroxynitrite (PN;25-200 uM), compared to 17-AAG or vehicle. G4 (25 uM) sensitized VSMC to apoptotic death (17.5% vs 8.8%, p=.02) following exposure to PN (300 uM, 4 hrs). In-vivo, G4 treatment resulted in increased apoptosis (TUNEL) in the ligated carotid artery (21% vs 4 %, p=.02) at 3 days, with no discernible difference in proliferation (Ki67 and BrdU). At 14 days, G4 treatment resulted in improved remodeling, with reduced intimal thickness (25%, p=.04) and increased radius:wall thickness ratio (50%; p=.008; Figure). Conclusions Selective targeting of mitochondrial Hsp90 sensitizes VSMC to mitochondrial depolarization and apoptosis, and alters vascular remodeling in-vivo. This may represent a new molecular target to modulate vascular injury.
Superresolution in MRI? (2002)

Recently, Peled and Yeshurun (1) investigated the possibility of calculating superresolution images from a set of spatially shifted, low-resolution images. Eight low-resolution images were acquired with an in-plane shift in phase encoding (PE) direction of 0, 1/4, 1/2, 3/4 pixel, and the same PE-shifts with an additional shift of 1/2 pixel in the read direction. Of course, images that are shifted by a fraction of one pixel dimension against each other look different and the subtraction of these images is not zero (or pure noise). The images are simply sampled at different positions in image space but the source data is identical. It is, therefore, not possible to increase resolution by acquiring images that are spatially shifted against each other, but it is obviously possible to increase the SNR. The images presented in Ref. 1 clearly show an increase in resolution. However, I think that this increase was probably achieved by the increased SNR and by correcting (amplifying) the T-decreased signal amplitude at the outer parts of k-space. I presume that the same results can be obtained by acquiring eight images with identical in-plane positions.
Susceptibility to Cardiac Arrhythmias and Sympathetic Nerve Growth in VEGF-B Overexpressing Myocardium (2020)

VEGF-B gene therapy is a promising proangiogenic treatment for ischemic heart disease, but, unexpectedly, we found that high doses of VEGF-B promote ventricular arrhythmias (VAs). VEGF-B knockout, alpha myosin heavy-chain promoter (αMHC)-VEGF-B transgenic mice, and pigs transduced intramyocardially with adenoviral (Ad)VEGF- B186 were studied. Immunostaining showed a 2-fold increase in the number of nerves per field (76 vs. 39 in controls, p < 0.001) and an abnormal nerve distribution in the hypertrophic hearts of 11- to 20-month-old αMHC-VEGF-B mice. AdVEGF-B186 gene transfer (GT) led to local sprouting of nerve endings in pig myocardium (141 vs. 78 nerves per field in controls, p < 0.05). During dobutamine stress, 60% of the αMHC-VEGF-B hypertrophic mice had arrhythmias as compared to 7% in controls, and 20% of the AdVEGF-B186-transduced pigs and 100% of the combination of AdVEGF-B186- and AdsVEGFR-1-transduced pigs displayed VAs and even ventricular fibrillation. AdVEGF-B186 GT significantly increased the risk of sudden cardiac death in pigs when compared to any other GT with different VEGFs (hazard ratio, 500.5; 95% confidence interval [CI] 46.4-5,396.7; p < 0.0001). In gene expression analysis, VEGF-B induced the upregulation of Nr4a2, ATF6, and MANF in cardiomyocytes, molecules previously linked to nerve growth and differentiation. Thus, high AdVEGF-B186 overexpression induced nerve growth in the adult heart via a VEGFR-1 signaling-independent mechanism, leading to an increased risk of VA and sudden cardiac death.
Proteomic analysis of UVC irradiation‐induced damage of plasma proteins: Serum amyloid P component as a major target of photolysis (2006)

Ultraviolet-C (UVC) irradiation is a pathogen inactivation method used for disinfection of pharmaceutical products derived from human blood. Previous studies have shown that UVC can potentially damage proteins through photolysis or can generate reactive species resulting in protein thiol oxidation. In this study, two fluorescence-based quantitative proteomic approaches were used to assess the effects of a novel UVC-disinfection strategy on human plasma fractions. We show minimal changes in protein content, but gross alterations in protein thiol reactivity, indicative of oxidative damage. We identify a number of the damaged proteins by mass spectrometry, including serum amyloid P component, and further demonstrate UVC-induced photolysis of its disulphide bond.
A Trimethoprim‐Based Chemical Tag for Live Cell Two‐Photon Imaging (2010)

Chemical tag for two-photon imaging: Here we developed a trimethoprim-based chemical tag that selectively labels intracellular proteins with a bright two-photon fluorophore. This chemical tag proved to be a viable tool for imaging proteins in live cells by using two-photon microscopy.
Photocontrol of selective light reflection by oblique helicoidal cholesteric (2021)

An oblique helicoidal cholesteric ChOH represents a unique optical material with a pitch that can be tuned by an electric or magnetic field in a broad range from sub micrometers to micrometers. In this work, we demonstrate that the oblique helicoidal cholesteric doped with azoxybenzene molecules and stabilized by an electric field could also be tuned by light irradiation. At a fixed voltage, UV irradiation causes a redshift of the reflection peak by more than 200 nm. The demonstrated effect has the potential for applications such as smart windows, sensors, tunable lasers, and filters.
Polymorphisms in genes encoding interleukin-10 and drug metabolizing enzymes GSTP1, GSTT1, GSTA1 and UGT1A1 influence risk and outcome in Hodgkin lymphoma (2012)

We genotyped 224 patients with Hodgkin lymphoma (HL) and 1056 healthy controls and related the risk for HL and outcome of chemotherapy treatment to polymorphisms in genes encoding interleukins and metabolizing enzymes by capillary electrophoresis. Patients with the UGT1A1 TA tandem repeat TA6/6 genotype had a poorer overall survival (OS) (relative risk [RR] 3.63, p = 0.004), and patients above 40 years with the GSTA1 AA genotype had poorer event-free survival (EFS) (RR 4.38, p = 0.003) after chemotherapy. In patients above 40 years, the IL-10 rs1800890 T-allele was associated with lower risk for HL (TT genotype vs. AA, odds ratio [OR] 0.38 [95% confidence interval 0.21-0.69], p = 0.001; AT/TT combined genotypes vs. AA, OR 0.45 [0.27-0.74], p = 0.001). The GSTP1 rs1695 A-allele reduced the risk for HL (GG vs. AG, OR 0.64 [0.42-0.99], p = 0.04; GG vs. AG/AA combined genotypes, OR 0.70 [0.47-1.04], p = 0.07), and the GSTT1 deleted genotype increased the risk for HL (OR 3.17 [1.97-5.09], p < 0.001) regardless of age.
Impact of CMP Design on High-Performance Embedded Computing (2006)

Chip multiprocessors (CMPs) will dominate commercial processor designs for at least the next decade, during which we will likely see an annual doubling of the number of processor cores integrated onto a single chip. This trend is
Estimation of Reservoir Properties from Seismic Attributes and Well Log Data Using Artificial Neural Networks (2010)

Porosity, permeability are key factors to build a 3D geological model for a reservoir. The best method&lt;br&gt;to get these properties would be to measure them on core samples in the laboratory. However, this&lt;br&gt;method is costly and time consuming, and usually only a few out of all wells are cored and even then&lt;br&gt;only a small portion of the well. To fill the gap in the vertical scale, geologists generally use a statistical&lt;br&gt;approach, such as linear or non-linear multiple regressions to correlate reservoir properties with the&lt;br&gt;continuously recorded well log data. Recently, geoscientists have utilized Artificial Intelligence (AI),&lt;br&gt;especially Neural Networks (ANNs), to predict reservoir properties. This talk reports a comparative&lt;br&gt;study of two types of neural networks, a Multiple-Layer Perception MLP, with back propagation neural&lt;br&gt;network, and a General Regression Neural Network GRNN. The viability of these techniques are&lt;br&gt;demonstrated on well log data and seismic attributes from sand stone reservoir in south of Algeria.&lt;br&gt;This study utilizes the basic logs including gamma ray GR, interval transit time DT, shale volume VSH,&lt;br&gt;bulk density RHOB, deep later log LLD and corrected porosity NPHI and five attributes( instantaneous&lt;br&gt;frequency, instantaneous phase, RMS amplitude, half energy and Arc length) to predict porosity,&lt;br&gt;permeability and lithofacies in cored and uncored wells. The agreement between the core data and the&lt;br&gt;predicted values by neural networks demonstrate a successful implementation and validation of the&lt;br&gt;network’s ability to map a complex non-linear relationship between well logs and permeability and&lt;br&gt;porosity. Also the results show that the application of the General Regression Neural Network GRNN&lt;br&gt;gives a relatively better performance than the Multiple-Layer Perception MLP.
How many human proteoforms are there (2018)

Despite decades of accumulated knowledge about proteins and their post-translational modifications (PTMs), numerous questions remain regarding their molecular composition and biological function. One of the most fundamental queries is the extent to which the combinations of DNA-, RNA- and PTM-level variations explode the complexity of the human proteome. Here, we outline what we know from current databases and measurement strategies including mass spectrometry-based proteomics. In doing so, we examine prevailing notions about the number of modifications displayed on human proteins and how they combine to generate the protein diversity underlying health and disease. We frame central issues regarding determination of protein-level variation and PTMs, including some paradoxes present in the field today. We use this framework to assess existing data and to ask the question, "How many distinct primary structures of proteins (proteoforms) are created from the 20,300 human genes?" We also explore prospects for improving measurements to better regularize protein-level biology and efficiently associate PTMs to function and phenotype.
Protective Effects of Anthocyanins against Amyloid β-Peptide-Induced Damage in Neuro-2A Cells (2011)

Alzheimer's disease is neuropathologically characterized by amyloid β-protein (Aβ) deposition, resulting in neurotoxicity. Herein, we focused on the prevention of anthocyanins from amyloid-mediated neurodysfunction. The data demonstrated that combined exposure of Aβ(1-40) and Aβ(25-35) to Neuro-2A cells resulted in reactive oxygen species (ROS) production and perturbation of calcium homeostasis. The expressions of LXRα, ApoE, ABCA1, and seladin-1 genes were significantly down-regulated upon Aβ challenge. β-Secretase, the rate-limiting enzyme that catalyzes amyloid precursor protein transform to Aβ, was up-regulated by Aβ treatment. For the duration of Aβ stimulation, malvidin (Mal) or oenin (Oen; malvidin-3-O-glucoside) was added, and the protective effects were observed. Mal and Oen showed protective effects against Aβ-induced neurotoxicity through blocking ROS formation, preserving Ca(2+) homeostasis, and preventing Aβ-mediated perturbation of certain genes involved in Aβ metabolism and cellular defense. The present study implicates anthocyanin as a potential therapeutic candidate for the prevention of amyloid-mediated neurodysfunction.
MVS Dynamic Reconfiguration Management (1992)

This paper presents an overview of the Dynamic Reconfiguration Management (DRM) function of MVS/ESA™ and its support of the IBM Enterprise System/9000™ family of machines. Dynamic Reconfiguration Management is the ability to select a new I/O configuration definition without needing to perform a power-on reset (POR) of the hardware or an initial program load (IPL) of the MVS operating system. Dynamic Reconfiguration Management allows the installation to add, delete, or modify definitions for channel paths, control units, and I/O devices, in both the software and hardware I/O configurations.
Dynamics of anticipatory mechanisms during predictive context processing (2012)

We employed an electroencephalography paradigm manipulating predictive context to dissociate the neural dynamics of anticipatory mechanisms. Subjects either detected random targets or targets preceded by a predictive sequence of three distinct stimuli. The last stimulus in the three-stimulus sequence (decisive stimulus) did not require any motor response but 100% predicted a subsequent target event. We showed that predictive context optimises target processing via the deployment of distinct anticipatory mechanisms at different times of the predictive sequence. Prior to the occurrence of the decisive stimulus, enhanced attentional preparation was manifested by reductions in the alpha oscillatory activities over the visual cortices, resulting in facilitation of processing of the decisive stimulus. Conversely, the subsequent 100% predictable target event did not reveal the deployment of attentional preparation in the visual cortices, but elicited enhanced motor preparation mechanisms, indexed by an increased contingent negative variation and reduced mu oscillatory activities over the motor cortices before movement onset. The present results provide evidence that anticipation operates via different attentional and motor preparation mechanisms by selectively pre-activating task-dependent brain areas as the predictability gradually increases.
Single‐Sperm Typing (2002)

Abstract This unit presents protocols for sperm isolation using two different methods, amplification of simple sequence‐length polymorphisms (SSLP) and/or single nucleotide polymorphisms (SNP) from single cells or whole genome‐amplified single cells using primer extension preamplification (PEP), and discusses the statistical analysis of sperm‐typing recombination data. Newer methods for studying recombination over very short distances (a few kilobases) using total sperm DNA and allele‐specific PCR are also discussed.
Lithium Detection in the Electron Microscope (2016)

This paper will present the results for the determination of concentration of Li in silicates cathode materials using x-ray microanalysis with windowless SDD EDS technology and with Electron Energy Loss Spectroscopy (EELS) with state of the art field emission scanning electron microscopes at high spatial resolution. Advantages and disadvantages of both techniques will be covered. The aim of this work is to characterize Li concentration variation at the nm scale in batteries materials. The issue of electron beam damage will be covered and the advantage to work at electron beam energies below 30 keV will be demonstrated.
Recreational Applications of OpenViBE: Brain Invaders and Use-the-Force (2016)

International audience
Comprehensive study combining surface science and real catalyst for NO direct decomposition (2002)

The catalytic activity of Pd/Al2O3 prepared from various palladium precursors for direct NO decomposition is closely related to the fraction of surface step sites capable of dissociating NO, on the basis of a surface science study using single-crystal model catalyst.
[Cardio-Pulmonary-Renal interactions]. (2017)

Over the past decade, understanding about feedback mechanisms involving the heart, lung and kidney is significantly improved. Each organ injury may trigger hemodynamic, neuro-hormonal and cellular pathway that may damage diverse organs. Recurrent acute on chronic injury may lead to the advanced stage of disease. On the other hand, chronic pathological conditions may decrease functional reserve leading to a high susceptibility to acute injury. Assessment of functional reserve and dosage of novel biomarkers may allow an early diagnosis and treatment. This review summarizes the current state-of-the-art understanding of cardio-pulmonary-renal interactions.
BLAST: THE MASS FUNCTION, LIFETIMES, AND PROPERTIES OF INTERMEDIATE MASS CORES FROM A 50 deg2SUBMILLIMETER GALACTIC SURVEY IN VELA (ℓ ≈ 265°) (2009)

We present first results from an unbiased 50 deg^2 submillimeter Galactic survey at 250, 350, and 500 micron from the 2006 flight of the Balloon-borne Large Aperture Submillimeter Telescope (BLAST). The map has resolution ranging from 36 arcsec to 60 arcsec in the three submillimeter bands spanning the thermal emission peak of cold starless cores. We determine the temperature, luminosity, and mass of more than one thousand compact sources in a range of evolutionary stages and an unbiased statistical characterization of the population. From comparison with C^(18)O data, we find the dust opacity per gas mass, kappa r = 0.16 cm^2 g^(-1) at 250 micron, for cold clumps. We find that 2% of the mass of the molecular gas over this diverse region is in cores colder than 14 K, and that the mass function for these cold cores is consistent with a power law with index alpha = -3.22 +/- 0.14 over the mass range 14 M_sun &lt; M &lt; 80 M_sun. Additionally, we infer a mass-dependent cold core lifetime of t_c(M) = 4E6 (M/20 M_sun)^(-0.9) years - longer than what has been found in previous surveys of either low or high mass cores, and significantly longer than free fall or likely turbulent decay times. This implies some form of non-thermal support for cold cores during this early stage of star formation.
Development of Polynomial Chaos Based Surrogate Models for Channel Simulation (2018)

Improvements in high frequency signal transmission places requirements on meticulous jitter modeling and prediction techniques, which have to provide precision below 1 ps. In general timing jitter is divided into random and deterministic jitter, where the latter includes data dependent jitter, which is the focus of this paper, since it can be the dominant cause of jitter and it has proven to be challenging to model. Traditionally, jitter arising in eye-diagrams of high speed channels is modeled using a transient simulation with a length of thousands or millions of bits. This approach can be very time consuming. Hence, statistical eye analysis techniques are developed, providing for fast prediction of eye diagram and jitter [1]. However these techniques are only applicable to linear systems, which can be an important constituent for jitter in high speed channel simulation.
A 500 MS/s 76dB SNDR continuous time delta sigma modulator with 10MHz signal bandwidth in 0.18μm CMOS (2013)

A 5 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">th</sup> order continuous time delta sigma modulator is designed in 0.18μm CMOS. At a sampling rate of 500MHz it achieves 76dB SNDR over a 10MHz bandwidth consuming 58mW. 5 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">th</sup> order noiseshaping is realized with 4 opamp based RC integrators and a VCO realizing an integrator and a 4 bit quantizer. A THD of −82.3dBc is achieved without calibration of feedback DACs. A high speed capacitive implementation of excess loop delay compensation, together with a method for reducing the switching activity of the output codes from the VCO are proposed.
Books and Software: Powerful Statistics with SYSTAT (2000)

ADVERTISEMENT RETURN TO ISSUEPREVDepartmentsNEXTBooks and Software: Powerful Statistics with SYSTATA review of SYSTAT 9.0Paul J. GemperlineCite this: Anal. Chem. 2000, 72, 9, 362 APublication Date (Web):May 1, 2000Publication History Published online1 May 2000Published inissue 1 May 2000https://doi.org/10.1021/ac002792l. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views392Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (220 KB) Get e-Alertsclose SUBJECTS:Mathematical methods,Software Get e-Alerts
The neutrophil-activating protein (HP-NAP) of Helicobacter pylori is a potent stimulant of mast cells (2002)

Infection by Helicobacter pylori causes an acute inflammatory response followed by a chronic infection of the human gastric mucosa. A neutrophil-activating protein (HP-NAP) has been identified in H.pylori, and its role in infection and immune response is currently under investigation. Here, we show that HP-NAP induces beta-hexosaminidase release and interleukin-6 production in peritoneal mast cells, two actions which are completely inhibited by pertussis toxin. We also show that in polarized epithelial cell monolayers HP-NAP translocates from the apical to the basolateral domain, where mast cells are located. These findings characterize HP-NAP as an inflammatory factor of H.pylori that is effective from the beginning of the inflammatory cascade.
Silica Nanorod-Array Films with Very Low Refractive Indices (2005)

The refractive-index contrast is an important figure of merit for dielectric multilayer structures, optical resonators, and photonic crystals. This represents a strong driving force for novel materials that have refractive indices lower than those of conventional optically transparent materials. Silica nanorod-array dielectric films with unprecedented low refractive indices of 1.08 are demonstrated and shown to have viable optical properties including enhanced reflectivity of a single-pair distributed Bragg reflector.
Light-controlled DNA binding of bisbenzamidines (2011)

Caging of the amidinium moieties of two representative bisbenzamidine DNA binders with suitable photoresponsive protecting groups suppresses their DNA-binding, which can then be fully recovered upon irradiation with UV light that removes the caging groups, regenerating the parent compounds. Moreover, a caged aza-pentamidine (©1a) is inert against Candida albicans, but displays a potent growth inhibitory activity after irradiation with UV light.