gebhart/patrepeval huggingface dataset:
Decay of prepulse facilitation of N type calcium channels during G protein inhibition is consistent with binding of a single G βγ subunit (1998)

We have examined the modulation of cloned and stably expressed rat brain N type calcium channels (alpha1B + beta1b + alpha2delta subunits) by exogenously applied purified G protein betagamma subunits. In the absence of Gbetagamma, barium currents through N type channels are unaffected by application of strong depolarizing prepulses. In contrast, inclusion of purified Gbetagamma in the patch pipette results in N type currents that initially facilitated upon application of positive prepulses followed by rapid reinhibition. Examination of the kinetics of Gbetagamma-dependent reinhibition showed that as the duration between the test pulse and the prepulse was increased, the degree of facilitation was attenuated in a monoexponential fashion. The time constant tau for the recovery from facilitation was sensitive to exogenous Gbetagamma, so that the inverse of tau linearly depended on the Gbetagamma concentration. Overall, the data are consistent with a model whereby a single Gbetagamma molecule dissociates from the channel during the prepulse, and that reassociation of Gbetagamma with the channel after the prepulse occurs as a bimolecular reaction.
Use of small intestinal submucosal implants for regeneration of large fascial defects: An experimental study in dogs (1999)

A single layer of porcine small intestinal submucosa (SIS) was sutured into a 5 x 5 cm window created in the fascia lata of ten adult mongrel dogs in order to determine the efficacy of this material in promoting tissue regeneration of large fascial defects. A similar defect in the contralateral limb was left empty and served as a negative control. Tissue regeneration was examined grossly and histologically at 6 and 12 weeks. By 6 weeks, marked fibroplasia and angiogenesis had occurred throughout the SIS scaffold. The regenerated tissue was well organized and showed good integration with the adjacent fascia while the control specimens were filled with loose areolar connective tissue. At 12 weeks the experimental defects were filled with a regenerated tissue that grossly and histologically resembled normal fascia. There was no evidence of adhesions to the underlying musculature. Conversely, the tissue that filled the control defects remained disorganized and was markedly thinner than the adjacent fascia. The results of this study suggest that SIS is capable of supporting tissue regeneration in large fascial defects. The ability of this material to induce regeneration of a substantial area of tissue grossly and histologically similar to normal fascia and without adhesions to the underlying musculature makes its application in reconstructive surgery appear promising.
A Decentralized B&B Algorithm for Motion Planning of Robot Swarms With Temporal Logic Specifications (2021)

In this letter, we study the problem of decentralized motion planning of robot swarms under high-level temporal logic specifications with a top-down approach. We use Swarm Signal Temporal Logic (SwarmSTL) to express swarm-level specifications. By encoding SwarmSTL formulas as mixed binary-integer constraints on the swarm features, the motion planning problem is formulated as a mixed-integer quadratic programming (MIQP) problem. We develop a decentralized Branch and Bound (B&B) algorithm with a node decentralization scheme such that the nodes in the B&B tree can be processed in parallel with communication among the agents and the agents can achieve consensus on the solution. Also, several search strategies to accelerate the decentralized B&B algorithm are proposed, and the performance improvements are presented. We evaluate the proposed algorithm using a supply transportation example with different formulas.
Nuclear Lamin Antigens are Developmentally Regulated during Porcine and Bovine Embryogenesis1 (1989)

The nuclear lamins, proteins that reside on the inner face of the nuclear envelope, are thought to provide attachment sites for anchoring the chromatin to the nuclear envelope, thus facilitating the overall organization of the nucleus. The composition of the nuclear lamin proteins changes during differentiation and development in a variety of mammalian and nonmammalian tissues. Bovine and porcine oocytes and early embryos were prepared for immunocytochemical detection of nuclear lamins using three different antibodies (recognizing lamin B, lamins A/B/C, or lamins A/C). In both species, germinal vesicle nuclei and early cleavage stage nuclei react positively with the antibodies. However, on nuclei of bovine embryos, the A/C epitope was not detectable at the 16-cell stage, compact morula, spherical blastocyst, or the chorionic cell nuclei of a Day 35 conceptus, but was detectable on both amniotic and embryonic ectodermal cell nuclei of a Day 35 conceptus. All three antibodies reacted with nuclei from two bovine tissue culture cell lines (bovine embryonic cells and Madin-Darby bovine kidney cells) and one porcine kidney cell line. Nuclei in porcine embryos followed a similar pattern, except the loss of the A/C epitope occurred at the 8-cell stage and the epitope was absent from compact morula and spherical blastocyst stage nuclei. All interphase nuclei in both species reacted with both anti-lamin A/B/C and anti-lamin B antibodies, whereas metaphase chromosomes did not react with any of the lamin antibodies tested. The change in recognizing the lamin epitope occurred one cell cycle after the expected transition from maternal control to zygotic control of development. Nuclear transplantation showed that 16-cell stage porcine nuclei, which are lamin A/C negative, acquired the A/C epitope after transfer to an enucleated metaphase II oocyte. These results suggest that the A/C epitope is developmentally regulated.
Initial cell search procedure in WCDMA - an improved algorithm for FDD (2003)

The initial search procedure in WCDMA is used to identify the scrambling code used by the base station that has the lowest path loss coefficient of the received signal, among all the other base stations. Before this action, the slot synchronization, frame synchronization and code-group identification must be performed. This paper deals with an optimized algorithm for the initial search procedure performed by a mobile station every time when it wants to access network services in UMTS. We have focused on the second step of the initial search procedure: frame synchronization and code-group identification. The algorithm minimizes the acquiring time of the scrambling code group, further used for identify the scrambling code of the selected base station.
CKD Increases the Risk of Age-Related Macular Degeneration (2008)

Age-related macular degeneration is the leading cause of irreversible blindness in the United States and often coexists with chronic kidney disease. Both conditions share common genetic and environmental risk factors. A total of 1183 participants aged 54+ were examined in the population-based, prospective cohort Blue Mountains Eye Study (Australia) to determine if chronic kidney disease increases the risk of age-related macular degeneration. Moderate chronic kidney disease (estimated glomerular filtration rate < 60 ml/min per 1.73 m(2) based on the Cockcroft-Gault equation) was present in 24% of the population (286 of 1183). The 5-yr incidence of early age-related macular degeneration was 3.9% in participants with no/mild chronic kidney disease (35 of 897) and 17.5% in those with moderate chronic kidney disease (50 of 286). After adjusting for age, sex, cigarette smoking, hypertension, complement factor H polymorphism, and other risk factors, persons with moderate chronic kidney disease were 3 times more likely to develop early age-related macular degeneration than persons with no/mild chronic kidney disease (odds ratio = 3.2; 95% confidence interval, 1.8 to 5.7, P < 0.0001). Each SD (14.8 ml/min per 1.73 m(2)) decrease in Cockcroft-Gault estimated glomerular filtration rate was associated with a doubling of the adjusted risk for early age-related macular degeneration (odds ratio = 2.0; 95% confidence interval, 1.5 to 2.8, P < 0.0001). In conclusion, persons with chronic kidney disease have a higher risk of early age-related macular degeneration, suggesting the possibility of shared pathophysiologic mechanisms between the two conditions.
Optical mammography instrument for broadband spectral imaging with depth discrimination (2015)

We are developing a new instrument for diffuse optical mammography in parallel plate geometry that operates over a broad spectral range of 600-1000 nm, features a scan time of 1-2 min, and allows for dynamic measurements at a selected region of interest. Furthermore, this new instrument is capable of depth discrimination of optical inhomogeneities embedded in the examined tissue by using multiple off-axis detection fibers. Using a solid silicone phantoms, mimicking breast tissue with 39 mm thickness, we demonstrate the capability of this instrument to recover the depth of blood-vessel-like structures to within ~2 mm. Additionally, we demonstrate the capability of this instrument to perform dynamic optical measurements with a temporal sampling rate as high as 20 Hz. We describe our plans to integrate this rich spectral, spatial, and temporal information into a single instrument for translation into clinical measurements on breast cancer patients.
Uranium hexamethoxide and mixed methoxyuranium(VI) fluorides: facile syntheses from uranium hexafluoride and a nuclear magnetic resonance investigation of structure and chemical dynamics (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTUranium hexamethoxide and mixed methoxyuranium(VI) fluorides: facile syntheses from uranium hexafluoride and a nuclear magnetic resonance investigation of structure and chemical dynamicsEdward A. Cuellar and Tobin J. MarksCite this: Inorg. Chem. 1981, 20, 7, 2129–2137Publication Date (Print):July 1, 1981Publication History Published online1 May 2002Published inissue 1 July 1981https://pubs.acs.org/doi/10.1021/ic50221a037https://doi.org/10.1021/ic50221a037research-articleACS PublicationsRequest reuse permissionsArticle Views112Altmetric-Citations26LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Crystallization studies on linear aliphatic n‐polyurethanes (2009)

Abstract A detailed crystallization study of the linear n ‐polyurethane ( n ‐PUR) family for n ranging from 5 to 12 was carried out by DSC supported by polarizing optical microscopy. The study embraces crystallization of all the n ‐PUR under both nonisothermal and isothermal conditions. The odd and even series of n ‐PUR defined by the parity of the number of methylenes ( n ) contained in the polymer repeating unit are considered and separately analyzed. All the members of the two series showed a thermal behavior consistent with their chemical constitution. Isothermal crystallization data were analyzed by the kinetics Avrami approach which revealed that the “crystallizability” of n ‐PUR increases steadily with the flexibility of the polyurethane chain. Melting and enthalpy temperatures of isothermally and nonisothermally crystallized n ‐PUR were found to vary with n according to a zig‐zag plot characteristic of odd–even effect. Given the structural similitude of n ‐PUR with ( n + 2)‐nylons, results were referenced to those reported for this family of polyamides. © 2009 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 47: 1368–1380, 2009
An intravenous DNA-binding priming agent protects cell-free DNA and improves the sensitivity of liquid biopsies (2023)

Blood-based, or "liquid," biopsies enable minimally invasive diagnostics but have limits on sensitivity due to scarce cell-free DNA (cfDNA). Improvements to sensitivity have primarily relied on enhancing sequencing technology <i>ex vivo</i> . Here, we sought to augment the level of circulating tumor DNA (ctDNA) detected in a blood draw by attenuating the clearance of cfDNA <i>in vivo</i> . We report a first-in-class intravenous DNA-binding priming agent given 2 hours prior to a blood draw to recover more cfDNA. The DNA-binding antibody minimizes nuclease digestion and organ uptake of cfDNA, decreasing its clearance at 1 hour by over 150-fold. To improve plasma persistence and limit potential immune interactions, we abrogated its Fc-effector function. We found that it protects GC-rich sequences and DNase-hypersensitive sites, which are ordinarily underrepresented in cfDNA. In tumor-bearing mice, priming improved tumor DNA recovery by 19-fold and sensitivity for detecting cancer from 6% to 84%. These results suggest a novel method to enhance the sensitivity of existing DNA-based cancer testing using blood biopsies.
Novel method for measurement of outer membrane permeability to new beta-lactams in intact Enterobacter cloacae cells (1991)

The ability of five new beta-lactams to permeate the outer membrane of intact Enterobacter cloacae beta-lactamase-overproducing cells was measured by using a high-pressure liquid chromatography (HPLC)-based technique that avoided certain possible artifacts of the traditional methods. Low concentrations of antibiotics were mixed with bacterial suspensions, and at different times, the cells were removed from the medium by filtration. Residual beta-lactam concentrations in the medium were then assessed by HPLC and UV detection. The assay was performed under conditions in which no beta-lactamase activity was detected in the filtrate and the number of viable cells remained constant during the experiment. Outer membrane permeability was assessed with the Zimmermann-Rosselet equation, in which outer membrane permeability was rate limiting for hydrolysis of the beta-lactam by periplasmic beta-lactamase. Thus, the rate of disappearance of beta-lactam was equal to the rate of outer membrane permeation. Preincubation of bacterial suspensions with 300 micrograms of cloxacillin per ml inhibited the hydrolysis of beta-lactams by intact cells, demonstrating that beta-lactam hydrolysis by periplasmic beta-lactamase was essential in order to allow measurement of outer membrane permeability by this method. Permeability coefficients (P) were calculated from the Zimmermann-Rosselet equation and were independent of the external concentration of antibiotic over a 100-fold concentration range. Cefepime and cefpirome exhibited rates of outer membrane permeation 5- to 20-fold higher than those of carumonam, ceftriaxone, and cefotaxime. Thus, the presence of a positive charge in the 3-lateral chain increased the permeation ability of beta-lactam molecules considerably.
Synthesis and Biologic Evaluation of Novel 18F-Labeled Probes Targeting Prostate-Specific Membrane Antigen for PET of Prostate Cancer (2016)

A novel <sup>18</sup>F-labeled asymmetric urea compound, <sup>18</sup>F-10a, had a high radiochemical yield, high binding affinity for PSMA, and pharmacokinetic profiles suitable for a PSMA imaging probe. We believe that <sup>18</sup>F-10a can be effectively and safely used in this type of imaging.
Characterisation and Distribution of Karaka Ōkahu Purepure Virus—A Novel Emaravirus Likely to Be Endemic to New Zealand (2021)

We report the first emaravirus on an endemic plant of Aotearoa New Zealand that is, to the best of our knowledge, the country's first endemic virus characterised associated with an indigenous plant. The new-to-science virus was identified in the endemic karaka tree (<i>Corynocarpus laevigatus</i>), and is associated with chlorotic leaf spots, and possible feeding sites of the monophagous endemic karaka gall mite. Of the five negative-sense RNA genomic segments that were fully sequenced, four (RNA 1-4) had similarity to other emaraviruses while RNA 5 had no similarity with other viral proteins. A detection assay developed to amplify any of the five RNAs in a single assay was used to determine the distribution of the virus. The virus is widespread in the Auckland area, particularly in mature trees at Ōkahu Bay, with only occasional reports elsewhere in the North Island. Phylogenetic analysis revealed that its closest relatives are pear chlorotic leaf spot-associated virus and chrysanthemum mosaic-associated virus, which form a unique clade within the genus <i>Emaravirus</i>. Based on the genome structure, we propose this virus to be part of the family <i>Emaravirus</i>, but with less than 50% amino acid similarity to the closest relatives in the most conserved RNA 1, it clearly is a novel species. In consultation with mana whenua (indigenous Māori authority over a territory and its associated treasures), we propose the name Karaka Ōkahu purepure virus in te reo Māori (the Māori language) to reflect the tree from which it was isolated (karaka), a place where the virus is prevalent (Ōkahu), and the spotted symptom (purepure, pronounced pooray pooray) that this endemic virus appears to cause.
Comparative measurement of photosensitizer triplet yield in vitro (1998)

The ratio of triplet state production has been estimated for two photosensitizers, using measurements of transient reflectance change from cellular suspensions. This estimate is a measure of the relative activity in vitro for two sensitizers which have the same cellular localization but different cytotoxicity.
ChemInform Abstract: Synthesis of 2,4,5‐Trisubstituted 3‐Fluorofurans via Sequential Iodocyclization and Cross‐Coupling of gem‐Difluorohomopropargyl Alcohols. (2008)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Heuristics for Evaluating IT Security Management Tools (2011)

The usability of IT security management (ITSM) tools is hard to evaluate by regular methods, making heuristic evaluation attractive. However, standard usability heuristics are hard to apply as IT security management occurs within a complex and collaborative context that involves diverse stakeholders. We propose a set of ITSM usability heuristics that are based on activity theory, are supported by prior research, and consider the complex and cooperative nature of security management. In a between-subjects study, we compared the employment of the ITSM and Nielsen's heuristics for evaluation of a commercial identity management system. Participants who used the ITSM set found more problems categorized as severe than those who used Nielsen's. As evaluators identified different types of problems with the two sets of heuristics, we recommend employing both the ITSM and Nielsen's heuristics during evaluation of ITSM tools.
Charge Transport of Polyester Ether Ionomers in Unidirectional Silica Nanopores (2016)

Dielectric relaxation spectroscopy is employed to investigate charge transport properties of two polyester ether ionomers in the bulk state and when confined in unidirectional nanoporous membranes (average pore diameter = 7.5 nm). Under nanometric confinement in nonsilanized pores, the macroscopic transport quantities (dc conductivity and characteristic frequency rate) are lower by about 1.4 decades compared to the bulk. The remarkable decrease of transport quantities in nonsilanized nanoporous membranes can be quantitatively explained by considering the temperature dependence of the interfacial layer between the ionomer and the silica membrane surfaces. On the other hand, an enhancement of dc conductivity is observed when the surfaces of the pores are treated with a nonpolar organosilane. This effect becomes more pronounced at lower temperatures and is attributed to slight changes in molecular packing density caused by the two-dimensional geometrical constraint.
Electronic beam steering using a varactor-tuned impedance surface (2002)

Limited electronic steering can be accomplished using a focusing reflective surface by changing the feed location or direction of incidence. In this paper we describe a method of electronic beam steering by using a tunable impedance surface. Since there are no moving parts or complicated feed networks, this method of reflective beam steering can have great utility for low-cost and lightweight scanning antennas.
Selective C‐Arylation of Free (NH)‐Heteroarenes via Catalytic C—H Bond Functionalization. (2003)

Abstract For Abstract see ChemInform Abstract in Full Text.
Appendix B Levenberg–Marquardt–More Optimization Algorithm (2016)

This algorithm assumes the optimization of a single-valued function E(X) made of a sum of squares:
Abstract 12955: A Novel Algorithm for Automated Titration of Autonomic Regulation Therapy for Heart Failure (2020)

Background: Autonomic Regulation Therapy (ART) using chronic vagus nerve stimulation (VNS) is a promising new therapy for patients with heart failure who remain symptomatic despite standard care. In the ANTHEM-HF Pilot Study, a therapeutic VNS intensity was successfully achieved in patients with HFrEF using manually programmed VNS up-titration. An algorithm has been developed for automatically intensifying VNS in small increments to a programmable target and capable of adjustments during up-titration process using a hand-held programmer or magnet. Methods: 6 healthy canines were implanted with an implantable pulse generator (IPG) and electrical lead for right cervical VNS. IPG programming at implant activated the TA algorithm with (1) a 1-week start delay; (2) initial stimulation intensity of 0.125 mA, 130 μsec pulse width, and 5 Hz frequency; (3) target stimulation intensity of 2.5 mA, 250 μsec pulse width, and 5 Hz frequency; and (4) trajectory to achieve the target intensity in approximately 10 weeks. Magnet placement over the IPG at scheduled intervals tested algorithm design intention to decrement VNS intensity, prolong the time to reach the target intensity, and/or temporarily inhibit VNS. Results: All animals underwent successful VNS system implantation and completed all scheduled activities. There was one transient implant-related adverse event (Horner’s Syndrome). The TA algorithm performed as designed. The targeted VNS intensity was achieved as scheduled, and automated titration was well tolerated in all animals. There were no stimulation-related adverse events. ECG monitoring demonstrated no clinically significant cardiac findings. Detailed gross necropsy and macroscopic examinations revealed vagus nerves and all major organs to be normal in all animals. Conclusion: An IPG for VNS having a programmable TA algorithm for automatically intensifying VNS to a target intensity and capable of adjustment in response to magnet applications to the IPG, was successfully tested in a preclinical study of healthy canines. The TA algorithm performed as designed, appears to be safe and could dramatically reduce burden of titration in a clinical setting.