1954 AGE ASSOCIATED CHANGES IN URINARY PROTEOME OF OAB PATIENTS (2011)

You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Non-Neurogenic Voiding Dysfunction1 Apr 20111954 AGE ASSOCIATED CHANGES IN URINARY PROTEOME OF OAB PATIENTS Vikas Tyagi, MD Harvey Qu, PhD Kenneth Peters, MD Yao-Chi Chuang, MD Hann-Chorng Kuo, MD Naoki Yoshimura, MD, PhD Shachi Tyagi, MD Michael Chancellor, andMD Pradeep TyagiPhD Vikas TyagiVikas Tyagi Royal Oak, MI More articles by this author , Harvey QuHarvey Qu Rochester, MI More articles by this author , Kenneth PetersKenneth Peters Royal Oak, MI More articles by this author , Yao-Chi ChuangYao-Chi Chuang Kaohsiung, Taiwan More articles by this author , Hann-Chorng KuoHann-Chorng Kuo Hualien, Taiwan More articles by this author , Naoki YoshimuraNaoki Yoshimura Pittsburgh, PA More articles by this author , Shachi TyagiShachi Tyagi Pittsburgh, PA More articles by this author , Michael ChancellorMichael Chancellor Royal Oak, MI More articles by this author , and Pradeep TyagiPradeep Tyagi Royal Oak, MI More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.2158AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Epidemiology studies consistently report increased prevalence of overactive bladder (OAB) with age, and aging population is increasing worldwide. Studies on aged rodent bladder have so far yielded contradictory results on age associated changes in muscarinic receptors, further highlighting the species differences in OAB pathology. We hypothesized that age related biochemical changes in bladder (decreased muscle mass, urothelial thinning and increased collagen) resulting in altered bladder function (overactive hyocontractile bladder) will be reflected in altered urinary proteome of elderly OAB patients. METHODS Single time point urine specimens were obtained from 74 OAB patients in the age range of ( 25-90 years) of either sex coming routinely to the two Urology clinics in Taiwan. Differentiation between OAB-wet and OAB-dry was based on symptoms and a 3-day voiding diary. Ten chemokines in urine were measured by MILLIPLEX™ MAP Human Cytokine/Chemokine multiplex immunoassay and ELISA.Multivariate and univariate statistical analysis was done to determine association of age with urinary chemokines and OAB symptoms. RESULTS In agreement with age dependent higher prevalence of OAB, the multivariate logistic regression analysis in our study also highlighted the significant association of OAB symptoms with age (odds ratio [OR]:1.12; 95% CI,(1.0729, 1.1872), p=0.0001. Univariate analysis of 11 urinary proteins revealed age associated elevation of four selected proteins in OAB. Urinay NGF had positive and modest correlation with age of OAB dry patients (Spearman r=0.3821; p<0.05)but there was no association with OAB wet patients. Analysis of MCP-1 and IP-10 in urine of OAB without separation into dry or wet condition also revealed association with age. The r=0.274 for MCP-1 (p<0.05) and r=0.250 for IP-10 (p<0.05 ). Multivariate modeling also revealed that the risk of having OAB (OR: 0.99; 95%CI,(0.9915, 0.9984), p=0.004) is slightly decreased with elevation of IL-1ra in urine. CONCLUSIONS Based on our findings, we postulate that age associated biochemical changes in the bladder are revealed by changes in urinary proteome especially in proteins that target specific components of bladder, such as the urothelium (IP-10), the afferent sensory nerve pathways (NGF and MCP-1. Age associated elevation of MCP-1 may be related to decreased muscle mass and increased fat in bladder in old OAB patients.Urinary NGF and MCP-1 can be surrogate markers for monitoring age associated biochemical changes and the effect of therapeutic interventions in OAB patients. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited byGriebling T (2014) Re: Association of Inflammaging (Inflammation + Aging) with Higher Prevalence of OAB in Elderly PopulationJournal of Urology, VOL. 193, NO. 2, (608-610), Online publication date: 1-Feb-2015. Volume 185Issue 4SApril 2011Page: e781 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Vikas Tyagi Royal Oak, MI More articles by this author Harvey Qu Rochester, MI More articles by this author Kenneth Peters Royal Oak, MI More articles by this author Yao-Chi Chuang Kaohsiung, Taiwan More articles by this author Hann-Chorng Kuo Hualien, Taiwan More articles by this author Naoki Yoshimura Pittsburgh, PA More articles by this author Shachi Tyagi Pittsburgh, PA More articles by this author Michael Chancellor Royal Oak, MI More articles by this author Pradeep Tyagi Royal Oak, MI More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...