gebhart/patrepeval huggingface dataset:
Optical imaging of Renilla luciferase, synthetic Renilla luciferase, and firefly luciferase reporter gene expression in living mice (2004)

We have recently demonstrated that Renilla luciferase (Rluc) is a promising bioluminescence reporter gene that can be used for noninvasive optical imaging of reporter gene expression in living mice, with the aid of a cooled charged couple device (CCD) camera. In the current study, we explore the expression of a novel synthetic Renilla luciferase reporter gene (hRluc) in living mice, which has previously been reported to be a more sensitive reporter than native Rluc in mammalian cells. We explore the strategies of simultaneous imaging of both Renilla luciferase enzyme (RL) and synthetic Renilla luciferase enzyme (hRL):coelenterazine (substrate for RL/hRL) in the same living mouse. We also demonstrate that hRL:coelenterazine can yield a higher signal when compared to Firefly luciferase enzyme (FL): D-Luciferin, both in cell culture studies and when imaged from cells at the surface and from lungs of living mice. These studies demonstrate that hRluc should be a useful primary reporter gene with high sensitivity when used alone or in conjunction with other bioluminescence reporter genes for imaging in living rodents.
Diagnostics of Highly Degraded Industrial Gas Turbines Using Bayesian Networks (2011)

This paper presents an offline fault diagnostics method for highly degraded industrial gas turbines. The method recasts gas path analysis to an inference problem using Bayesian networks where the health condition of each component is quantified in comparison to an expected value. The health parameters are inferred from available gas path measurements, which are sometimes erroneous due to sensor faults or miscalibration. The sensor errors should be inferred as well as the health parameters. Thus, typically in gas path analysis the unknowns are more than the knowns. To address this issue, the present method uses multiple Bayesian network models each of which contains a subset of the unknowns. Their results are averaged according to how much each of the models is supported by the data. Although this method has been reported successful for the faults affecting a few unknowns, its results are still less accurate and confident when it is applied to highly degraded gas turbines. Such gas turbines are likely to have health parameters deviated from the new and clean condition as well as have component faults and sensor errors. Because of this, the present method must infer too many unknowns at the same time to result in a solution with high confidence. In addition, this method cannot differentiate normal or expected degradation from an actual fault. These issues are resolved by fusing extra information to the method. First of all, a sensor calibration report, if available, eliminates the sensor errors from the unknowns. Consequently, the number of possible subsets decreases, and so does the number of Bayesian models. Second, a degradation model provides meaningful prior guesses for the health parameters. It is equivalent to change the point of reference from a brand new gas turbine to a normally degraded one. It will be demonstrated that the method accompanying with the degradation model and the sensor calibration report shows significant improvement in accuracy and confidence.
Supplementary Table S3 from ER-851, a Novel Selective Inhibitor of AXL, Overcomes Resistance to Antimitotic Drugs (2023)

&lt;p&gt;Supplementary Table S3&lt;/p&gt;
An Experimental Observation of the Disaggregated Nonlinear Interference Noise Generation (2022)

We experimentally measure the independent cross-channel NLI components in WDM flex-grid flex-rate multi-vendor comb at symbol rate up to 90 Gbaud propagating on a multi-span amplified line and compare the results to the GNPy prediction.
Blinking statistics in single semiconductor nanocrystal quantum dots (2001)

Statistical studies of fluorescence intermittency in single CdSe nanocrystal quantum dots (QD's) reveal a temperature-independent power-law distribution in the histogram of on and off times---the time periods before the QD turns from emitting to nonemitting (bright to dark) and vice versa. Every QD shows a similar power-law behavior for the off-time distribution regardless of temperature, excitation intensity, surface morphology or size. We propose a dynamic model of tunneling between core and trapped charged states to explain the universal power-law statistics of the blinking events observed. The on-time probability distributions show evidence of both a tunneling mechanism similar to the off-time statistics and a secondary, photoinduced process that leads to a truncation of the power law. The same blinking statistics are also observed for single CdTe nanocrystal QD's.
Treated Colloidal Silica Particles for Toner Formulations (2006)

Surface modified silica particles are used as external additives to toners to improve free flow, to reduce agglomeration of toner particles, and to modify the tribo-charging characteristics of the final toner formulation. In many of the new toner formulations, large diameter spherical silica particles, rather than the traditional aggregated fumed silicas, are needed to give the required performance characteristics to the toner. In fact, large (order 100 nm) spherical silica particles have been cited as an essential additive in preventing agglomeration in new toner formulations [1].The manufacture of large treated spherical silica particles presents a variety of chemistry and processing challenges. Both the reaction and drying conditions during manufacture must be carefully controlled to produce powders with uniform treatment and minimal aggregation. In this paper, we shall discuss the effects of the treatment chemistry and the base silica particle size on toner tribo-charging and humidity stability and toner free flow for a model toner system.
Precision rotationally invariant curve parameterization (2009)

The problem of rotationally invariant approximation of the curve given by points is considered. The arc length is chosen for the curve parameter. The algorithm of calculating it up to the fourth order of accuracy is developed. In addition, other characteristics of the curve are constructed, viz., the slope, the curvature, and its derivative. General requirements to parametric approximation of the curve are considered that provide for rotationally invariant approximation.
Extraction of speculative SOI MOSFET models using self-heating-free targets (2008)

Speculative SPICE models (also referred to as evaluation-level or guess models), which are extracted based on projected device electrical characteristics (called `targets¿) rather than actual measurement data, are required to support concurrent IC designs. The self-heating effect in silicon-on-insulator (SOI) technologies presents additional challenges in obtaining quality speculative SOI MOSFET models. A novel `shift-and-ratio¿ technique is developed to generate self-heating free device targets from raw targets provided only at room temperature, and a corresponding speculative model extraction methodology is proposed. The shift-and-ratio technique is validated by using silicon data of 65 nm partially-depleted (PD) SOI technologies. The adequacy and self-consistency of the speculative model extraction methodology is demonstrated in field testing where a large number of 65 nm and 45 nm PD SOI speculative models are extracted. Availability of self-heating free targets proves to be critical not only for improved speculative model extraction efficiency, but also for model quality in general by ensuring physical and reasonable temperature dependences in the resulting speculative models.
The H.264/AVC Video Coding Standard [Standards in a Nutshell] (2007)

This article provides a description of the structure, technology, performance, and resources of H.264/AVC, which is referred to formally as ITU-T Recommendation H.264 and ISO/IEC 14496-10 (MPEG-4 Part 10)
Temporally-Consistent Surface Reconstruction using Metrically-Consistent\n Atlases (2021)

We propose a method for unsupervised reconstruction of a\ntemporally-consistent sequence of surfaces from a sequence of time-evolving\npoint clouds. It yields dense and semantically meaningful correspondences\nbetween frames. We represent the reconstructed surfaces as atlases computed by\na neural network, which enables us to establish correspondences between frames.\nThe key to making these correspondences semantically meaningful is to guarantee\nthat the metric tensors computed at corresponding points are as similar as\npossible. We have devised an optimization strategy that makes our method robust\nto noise and global motions, without a priori correspondences or pre-alignment\nsteps. As a result, our approach outperforms state-of-the-art ones on several\nchallenging datasets. The code is available at\nhttps://github.com/bednarikjan/temporally_coherent_surface_reconstruction.\n
Protein Biosynthesis in Escherichia coli (1970)

Abstract Five mutant strains of Escherichia coli were selected for resistance to the steroid antibiotic fusidic acid. The protein synthetic activity of the cell-free extracts of only one of these was resistant to the inhibitory effect in vitro of the antibiotic. G factor, one of the soluble proteins required for peptide chain elongation, was purified to virtual homogeneity from both sensitive parent and resistant mutant strains. Aside from their differing sensitivity to fusidic acid inhibition, the two G factors were indistinguishable by conventional immunological, chromatographic, and electrophoretic analyses. The two factors differed, however, in two other respects. The specific activity of the sensitive strain was approximately twice that of the resistant strain throughout purification. In addition, the Michaelis constant for the ribosomal dependent hydrolysis of GTP calculated for the sensitive factor was half that for the resistant. The study, therefore, characterizes this fusidic acid-resistant mutation as one affecting G factor and establishes a biochemical basis for further genetic studies. It also suggests that the active enzymatic site for the ribosome-dependent hydrolysis of GTP is associated with the soluble G fraction.
RNAscope in situ hybridization-based method for detecting DUX4 RNA expression in vitro (2019)

Facioscapulohumeral muscular dystrophy (FSHD) is among the most common forms of muscular dystrophy. FSHD is caused by aberrant expression of the toxic <i>DUX4</i> gene in muscle. Detecting endogenous <i>DUX4</i> in patient tissue using conventional methods can be challenging, due to the low level of <i>DUX4</i> expression. Therefore, developing simple and trustworthy <i>DUX4</i> detection methods is an important need in the FSHD field. Here, we describe such a method, which uses the RNAscope assay, an RNA in situ hybridization (ISH) technology. We show that a custom-designed RNAscope assay can detect overexpressed <i>DUX4</i> mRNA in transfected HEK293 cells and endogenous <i>DUX4</i> mRNA in FSHD patient-derived myotubes. The RNAscope assay was highly sensitive for tracking reductions in <i>DUX4</i> mRNA following treatment with our therapeutic mi405 microRNA, suggesting that RNAscope-based <i>DUX4</i> expression assays could be developed as a prospective outcome measure in therapy trials. This study could set the stage for optimizing and developing a new, rapid RNA ISH-based molecular diagnostic assay for future clinical use in the FSHD field.
A preformed, topologically stable replication fork. Characterization of leading strand DNA synthesis catalyzed by T7 DNA polymerase and T7 gene 4 protein. (1983)

This paper describes the construction of a DNA molecule containing a topologically stable structure that simulates a replication fork. This preformed DNA molecule is a circular duplex of 7.2 X 10(3) base pairs (M13mp6 DNA) from which arises, at a unique BamHI recognition site, a noncomplementary 5'-phosphoryl-terminated single strand of 237 nucleotides (SV40 DNA). This structure has two experimental attributes. 1) Templates for both leading and lagging strand synthesis exist as stable structures prior to any DNA synthesis. 2) DNA synthesis creates a cleavage site for the restriction endonuclease BamHI. Form I of T7 DNA polymerase, alone, catalyzes limited DNA synthesis at the preformed replication fork whereas Form II, alone, polymerizes less than 5 nucleotides. However, when T7 gene 4 protein is present, Form II of T7 DNA polymerase catalyzes rapid and extensive synthesis via a rolling circle mode. Kinetic analysis of this synthesis reveals that the fork moves at a rate of 300 bases/s at 30 degrees C. We conclude that the T7 gene 4 protein requires a single-stranded DNA binding site from which point it translocates to the replication fork where it functions as a helicase. The phage T4 DNA polymerase catalyzes DNA synthesis at this preformed replication fork in the presence of gene 4 protein, but the amount of DNA synthesized is less that 3% of the amount synthesized by the combination of Form II of T7 DNA polymerase and gene 4 protein. We conclude that T7 DNA polymerase and T7 gene 4 protein interact specifically during DNA synthesis at a replication fork.
Consolidation of Al/Fe3O4 composites using high-pressure torsion and their magnetic properties (2012)

Severe plastic deformation using high-pressure torsion was applied for consolidation of Al/Fe3O4 composites. The consolidation was achieved with the Vickers microhardness saturated to ~240 HV for Al–10 vol%Fe3O4 and to ~300 HV for Al–20 vol%Fe3O4 after processing for 5 revolutions under a pressure of 6 GPa. The density increased with straining and reached the highest after 5–7 revolutions but decreased for further revolutions. The coercive force had the same trend as the density but the saturation magnetization is almost invariable with straining. No appreciable difference was observed between the magnetic fields applied in the directions perpendicular and parallel to the ring plane. Electrical conductivity decreased with straining and this was attributed to generation of a large amount of lattice defects such as grain boundaries.
An amperometric affinity penicillin-binding protein magnetosensor for the detection of β-lactam antibiotics in milk (2013)

The preparation, characterization and performance evaluation of an amperometric affinity disposable magnetosensor, based on the use of a recombinant penicillin-binding protein (PBP) and screen-printed carbon electrodes (SPCEs), for the specific detection and quantification of β-lactam antibiotic residues in milk are reported. The PBP was immobilized onto His-Tag-Isolation-modified magnetic beads (His-Tag-Isolation-MBs), and a direct competitive assay using a tracer with horseradish peroxidase (HRP) for the enzymatic labeling was performed. The amperometric response obtained at -0.20 V vs. the Ag pseudo-reference electrode of the SPCE after the addition of H2O2 in the presence of hydroquinone (HQ) was used as the transduction signal. The developed methodology showed very low detection limits (in the low ppb level) for the 6 antibiotics tested in untreated milk samples, and a good selectivity against other antibiotic residues frequently detected in milk and dairy products. Due to the bioreceptor employed, this methodology was able to detect only the active form of β-lactam antibiotics with high affinities for both penicillins and cephalosporins. Moreover, the analysis took only 30 min.
Compact physical models for multiwall carbon-nanotube interconnects (2006)

Compact physical models are derived for conductivity of multiwall carbon-nanotube (MWCN) interconnects. It is proven that for MWCNs shorter than the critical length (typically around 7 μm), the conductivity decreases as diameter increases, whereas for MWCNs longer than the critical length, increasing the diameter results in higher conductivities. For long lengths (hundreds of micrometers), MWCNs can potentially have conductivities several times larger than that of copper or even single-wall carbon nanotube (SWCN) bundles. For short lengths (<10 μm), however, SWCN bundles offer more than two times higher conductivities compared to MWCNs.
Designing and Utilization of siRNAs Targeting RNA Binding Proteins (2008)

Small interfering RNA (siRNA)-mediated RNA interference (RNAi) is a very powerful tool for triggering posttranscriptional gene silencing in several organisms. We discuss the improvement of two different sources of siRNAs synthesized either chemically or by an enzymatic method. When the siRNAs are synthesized by in vitro transcription using a phage polymerase, the initiating triphosphates trigger a potent interferon induction that can lead to misinterpretation of the data. A novel method is presented to minimize the nonspecific effect of enzymatic siRNAs while maintaining the advantages of lower cost and less turnaround time. When chemical siRNAs are used, the expense and long turnaround time can be a problem, especially if the selected siR-NAs are not highly functional in triggering RNAi. The new format for making double-stranded RNAs (dsRNAs) is described to achieve more efficient suppression. The format has been tested by creating siRNAs targeting two RNA binding proteins, La and hnRNP (heterogeneous nuclear ribonucleoprotein) H, and has shown better potency at lower concentrations than the conventional 21-mer siRNA.
Adenovirus‐mediated shRNA Delivery to Cancer (2013)

Vector-based approaches have the potential to provide stable introduction of RNAi. In principle, it requires only a single treatment with shRNA vector to target a specific gene. This chapter describes novel strategies to develop more powerful shRNA expression vectors for the treatment of cancer, with a focus on adenoviral systems.
ChemInform Abstract: Novel Functionalized 1,4,4a,9a‐Tetrahydro‐1‐aza‐9‐oxafluorenes by Cycloaddition of 4‐(4‐Methoxyphenyl)‐1,4‐dihydropyridines and p‐Benzoquinone. (1998)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Antibiotics from basidiomycetes XLI. Clavicoronic acid, a novel inhibitor of reverse transcriptases from Clavicorona pyxidata (Pers. ex Fr.) Doty. (1992)

A novel inhibitor of RNA-directed DNA-polymerases was isolated from fermentations of Clavicorona pyxidata. Its structure was elucidated by spectroscopic methods. Clavicoronic acid (1) is a noncompetitive inhibitor of avian myeloblastosis virus (Ki 130 microM) and Moloney murine leukemia virus (Ki 68 microM) reverse transcriptases. In permeabilized cells and isolated nucleic DNA- and RNA-synthesis are not affected. Clavicoronic acid markedly inhibits the multiplication of vesicular stomatitis virus in baby hamster kidney cells by interfering with this virus's RNA-directed RNA-polymerase. 1 exhibits no cytotoxic and very weak antimicrobial activities.
Glycosaminoglycans in Human Trabecular Meshwork: Age-Related Changes (2004)

Glycosaminoglycans play a central role in maintaining the normal outflow resistance in the human trabecular meshwork. We evaluated the possible morphological, histochemical and morphometrical age-related changes in glycosaminoglycans of the trabecular meshwork. Small human samples were drawn from 24 eyes after exitus from young and old humans. Samples were harvested from the anterior chamber of the eye, without any aesthetic damage for the face. They were divided into three fragments, each used for morphological, histochemical and ultrastructural staining. Quantitative analysis of images was performed to evaluate morphometrical data that were statistically analysed. Our findings demonstrate the following age-related changes: (1) deposition of fibrous granular material in the trabecular meshwork; (2) increased electron density of the structures; (3) strong decrease in the hyaluronic acid content, and (4) increase in sulphated proteoglycans. Glycosaminoglycans of human trabecular meshwork undergo age-related changes, as demonstrated by our morphological, histochemical and morphometrical results.
Photochemical cyclohexane carbonylation cocatalyzed by d8 transition metal carbonyls and aromatic ketones and aldehydes (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhotochemical cyclohexane carbonylation cocatalyzed by d8 transition metal carbonyls and aromatic ketones and aldehydesWilliam T. Boese and Alan S. GoldmanCite this: J. Am. Chem. Soc. 1992, 114, 1, 350–351Publication Date (Print):January 1, 1992Publication History Published online1 May 2002Published inissue 1 January 1992https://pubs.acs.org/doi/10.1021/ja00027a049https://doi.org/10.1021/ja00027a049research-articleACS PublicationsRequest reuse permissionsArticle Views647Altmetric-Citations55LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
NEMS based logic and memory circuits (2012)

Carbon nanotube (CNT) based nano electromechanical system (NEMS) were developed to apply to the logic and the memory circuit. The electrical `on-off' behavior induced by the mechanical movements of CNTs can promise low power consumption in circuit with very low level leakage current. Additionally, the unique vertical structure of nanotubes allows high integration density for devices.
Secure Password-Based Cipher Suite for TLS. (2000)

SSL is the de facto standard today for securing endto -end transport. While the protocol seems rather secure there are a number of risks which lurk in its use, e.g., in web banking. We motivate the use of passwordbased key exchange protocols by showing how they overcome some of these problems. We propose the integration of such a protocol (DH-EKE) in the TLS protocol, the standardization of SSL by IETF. The resulting protocol provides secure mutual authentication and key establishment over an insecure channel. It does not have to resort to a PKI or keys and certificates stored on the users computer. Additionaly the integration in TLS is as minimal and non-intrusive as possible. As a side-effect we also improve DH-EKE to provide semantic security assuming the hardness of the Decisional Diffie-Hellman Problem. 1. Introduction The Secure Socket Layer (SSL) protocol [15] is todays de facto standard for securing end-to-end transport over the Internet. In particular the presence of SSL in ...