gebhart/patrepeval huggingface dataset:
Asciminib and ponatinib exert synergistic anti-neoplastic effects on CML cells expressing BCR-ABL1 T315I-compound mutations (2021)

Ponatinib is a tyrosine kinase inhibitor (TKI) directed against BCR-ABL1 which is successfully used in patients with BCR-ABL1 (T315I)+ chronic myeloid leukemia (CML). However, BCR-ABL1 compound mutations may develop during therapy in these patients and may lead to drug resistance. Asciminib is a novel drug capable of targeting most BCR-ABL1 mutant-forms, including BCR-ABL1(T315I), but remains ineffective against most BCR-ABL1(T315I)+ compound mutation-bearing sub-clones. We demonstrate that asciminib synergizes with ponatinib in inducing growth-arrest and apoptosis in patient-derived CML cell lines and murine Ba/F3 cells harboring BCR-ABL1 (T315I) or T315I-including compound mutations. Asciminib and ponatinib also produced cooperative effects on CRKL phosphorylation in BCR-ABL1-transformed cells. The growth-inhibitory effects of the drug combination ‘asciminib+ponatinib’ was further enhanced by hydroxyurea (HU), a drug which has lately been described to suppresses the proliferation of BCR-ABL1 (T315I)+ CML cells. Cooperative drug effects were also observed in patient-derived CML cells. Most importantly, we were able to show that the combinations ‘asciminib+ponatinib’ and ‘asciminib+ponatinib+HU’ produce synergistic apoptosis-inducing effects in CD34(+)/CD38(-) CML stem cells obtained from patients with chronic phase CML or BCR-ABL1 (T315I)+ CML blast phase. Together, asciminib, ponatinib and HU synergize in producing anti-leukemic effects in multi-resistant CML cells, including cells harboring T315I+ BCR-ABL1 compound mutations and CML stem cells. The clinical efficacy of this TKI combination needs to be evaluated within the frame of upcoming clinical trials.
Molecular cell biology and physiology of solute transport (1993)

Brigham and Women's Hospital, Boston, Massachusetts, USA Steven R. Gullans, PhD, and Steven C. Hebert, MD, Renal Division, Department of Medicine, Brigham and Women's Hospital, 75 Francis Street, Boston, MA 02115, USA.
Compact polarization rotator based on surface plasmon porariton with low insertion loss (2012)

We propose an ultra-small polarization rotator based on a surface plasmon polariton phenomenon. Numerical simulations show that a 10-μm-long polarization rotator with extinction ratio about -20 dB on the entire C-band is achievable.
A dual‐tuned 17O/1H head array for direct brain oximetry at 3 Tesla (2019)

A dual-tuned <sup>17</sup> O/<sup>1</sup> H array was constructed to enable high-sensitivity <sup>17</sup> O and <sup>1</sup> H imaging under standard clinical 3 T scanning conditions.
Re: Sphaeropsidin A Shows Promising Activity against Drug-Resistant Cancer Cells by Targeting Regulatory Volume Increase (2016)

No AccessJournal of UrologyInvestigative Urology1 Jul 2016Re: Sphaeropsidin A Shows Promising Activity against Drug-Resistant Cancer Cells by Targeting Regulatory Volume Increase Anthony AtalaMD Anthony AtalaAnthony Atala More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.03.156AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "Re: Sphaeropsidin A Shows Promising Activity against Drug-Resistant Cancer Cells by Targeting Regulatory Volume Increase." The Journal of Urology, 196(1), pp. 280–281 © 2016 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 196Issue 1July 2016Page: 280-281 Advertisement Copyright & Permissions© 2016 by American Urological Association Education and Research, Inc.MetricsAuthor Information Anthony Atala More articles by this author Expand All Advertisement PDF downloadLoading ...
Fourier transform spectrometer based on a rapidly cavity length modulated mode-locked fiber laser (2010)

We demonstrate a Fourier transform spectrometer based on a single repetition rate modulated mode-locked Yb-fiber laser configured as a coherent scanning delay line using an imbalanced Mach-Zehnder interferometer. The effective mirror scan rate is 3.75 m/s.
An Improved Inter-cell Interference Coordination (ICIC) for OFDMA multi-cell systems (2013)

In this paper, we propose an improved Inter-Cell Interference Coordination (ICIC) for emerging multi-cell wireless systems. The ICIC provided in this paper gives a proper inter-cell resource allocation by employing the concept of interference avoidance and describes the inter-relationship of all base stations in a holistic way. We use a semi-centralized ICIC scheme which divides the global optimum problem into a multicell and multi-user problem. Our performance metrics are the aggregate sector throughput and the critical throughput of a multi-cell system. The critical throughput is defined as the 5th percentile of the aggregate user throughput. Simulation results confirm the increased effectiveness of the proposed ICIC in both metrics over a number of state-of-the-art interference avoidance schemes. Keywords - component; Inter-cell RRM; Semi-centralized RRM; Linear/Binary Optimization; Inter-Cell Interference Coordination.
Impact of Dynamic Computed Tomographic Angiography on Endograft Sizing for Endovascular Aneurysm Repair (2009)

The observed changes in aneurysm dimension during the cardiac cycle are small and in the range of intraobserver variability, so dynamic changes in proximal aneurysm neck diameter and aneurysm length likely have little impact on preoperative endograft selection. However, changes in diameter at the distal sealing zones may be relevant to sizing, so distal oversizing of up to 20% should be considered to prevent distal type I endoleak.
Nagelamide J, a Novel Dimeric Bromopyrrole Alkaloid from a Sponge Agelas Species (2007)

A novel dimeric bromopyrrole alkaloid, nagelamide J (1), with antimicrobial activity has been isolated from an Okinawan marine sponge Agelas species, and the structure and stereochemistry were elucidated from spectroscopic data. Nagelamide J (1) is the first bromopyrrole alkaloid possessing a cyclopentane ring fused to an amino imidazole ring.
Nanoceria: factors affecting its pro- and anti-oxidant properties (2014)

Nanoceria redox properties are affected by particle size, particle shape, surface chemistry, and other factors, such as additives that coat the surface, local pH, and ligands that can participate in redox reactions. Each CeO2 crystal facet has a different chemistry, surface energy, and surface reactivity. Unlike nanoceria's industrial catalytic applications, biological and environment exposures are characterized by high water activity values and relatively high oxygen activity values. Electrochemical data show that oxygen levels, pH, and redox species affect its phase equilibria for solution and dissolution. However, not much is known about how the many and varied redox ligands in environmental and biological systems might affect nanoceria's redox behaviour, the effects of coated surfaces on redox rates and mechanisms, and whether the ceria solid phase undergoes dissolution at physiologically relevant pH and oxygen levels. Research that could answer these questions would improve our understanding of the links between nanoceria's redox performance and its morphology and environmental conditions in the local milieu.
High dosage of zinc modulates T-cells in a time-dependent manner within porcine gut-associated lymphatic tissue (2018)

Zn serves as a powerful feed additive to reduce post-weaning diarrhoea in pigs. However, the mechanisms responsible for Zn-associated effects on the adaptive immune responses following feeding of a very high dosage of Zn remain elusive. In this study, we examined the T-cell response in gut-associated lymphatic tissues of seventy-two weaned piglets. Piglets received diets with 57 mg Zn/kg (low Zn concentration, LZn), 164 mg Zn/kg (medium Zn concentration, MZn) or 2425 mg Zn/kg (high Zn concentration, HZn) mg Zn/kg feed for 1, 2 or 4 weeks. We observed that feeding the HZn diet for 1 week increased the level of activated T-helper cells (CD4+ and CD8α dim) compared with feeding MZn and LZn (P<0·05). In addition, we observed higher transcript amounts of interferon γ and T-box 21 (TBET) in the HZn group compared with the MZn and LZn groups (P<0·05). A gene set enrichment analysis revealed an over-representation of genes associated with 'cytokine signalling in immune system'. Remarkably, feeding of a very high Zn dosage led to a switch in the immune response after 2 weeks. We detected higher relative cell counts of CD4+CD25high regulatory T-helper cells (P<0·05) and a higher expression of forkhead box P3 (FOXP3) transcripts (P<0·05). After 4 weeks of feeding a high-dosage Zn diet, the relative CD4+ T-cell count (P<0·05) and the relative CD8β + T-cell count (P<0·1) were reduced compared with the MZn group. We hypothesise that after 1 week the cellular T-helper 1 response is switched on and after 2 weeks it is switched off, leading to decreased numbers of T-cells.
Monolithically Integrated Coherent Receiver for Highly Linear Microwave Photonic Links (2007)

A compact monolithically integrated coherent receiver with feedback has been fabricated for linear optical phase demodulation. The photodiodes demonstrate a saturation current greater than 60 mA and an OIP3 of 35 dBm at 40 mA.
Quantitative segmentation of fluorescence microscopy images of heterogeneous tissue: Approach for tuning algorithm parameters (2013)

The combination of fluorescent contrast agents with microscopy is a powerful technique to obtain real time images of tissue histology without the need for fixing, sectioning, and staining. The potential of this technology lies in the identification of robust methods for image segmentation and quantitation, particularly in heterogeneous tissues. Our solution is to apply sparse decomposition (SD) to monochrome images of fluorescently-stained microanatomy to segment and quantify distinct tissue types. The clinical utility of our approach is demonstrated by imaging excised margins in a cohort of mice after surgical resection of a sarcoma. Representative images of excised margins were used to optimize the formulation of SD and tune parameters associated with the algorithm. Our results demonstrate that SD is a robust solution that can advance vital fluorescence microscopy as a clinically significant technology.
uPA deficiency exacerbates muscular dystrophy in MDX mice (2007)

Duchenne muscular dystrophy (DMD) is a fatal and incurable muscle degenerative disorder. We identify a function of the protease urokinase plasminogen activator (uPA) in mdx mice, a mouse model of DMD. The expression of uPA is induced in mdx dystrophic muscle, and the genetic loss of uPA in mdx mice exacerbated muscle dystrophy and reduced muscular function. Bone marrow (BM) transplantation experiments revealed a critical function for BM-derived uPA in mdx muscle repair via three mechanisms: (1) by promoting the infiltration of BM-derived inflammatory cells; (2) by preventing the excessive deposition of fibrin; and (3) by promoting myoblast migration. Interestingly, genetic loss of the uPA receptor in mdx mice did not exacerbate muscular dystrophy in mdx mice, suggesting that uPA exerts its effects independently of its receptor. These findings underscore the importance of uPA in muscular dystrophy.
Saponification of dimethyl cis-2,6-diphenyl-4-oxocyclohexane-1,1-dicarboxylate. A reinvestigation (1982)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSaponification of dimethyl cis-2,6-diphenyl-4-oxocyclohexane-1,1-dicarboxylate. A reinvestigationAlex T. Rowland, John A. Hohneker, Keith F. McDaniel, and David S. MooreCite this: J. Org. Chem. 1982, 47, 2, 301–306Publication Date (Print):January 1, 1982Publication History Published online1 May 2002Published inissue 1 January 1982https://pubs.acs.org/doi/10.1021/jo00341a024https://doi.org/10.1021/jo00341a024research-articleACS PublicationsRequest reuse permissionsArticle Views220Altmetric-Citations7LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Obesity-Associated Hepatic Steatosis, Somatotropic Axis Impairment, and Ferritin Levels Are Strong Predictors of COVID-19 Severity (2023)

The full spectrum of SARS-CoV-2-infected patients has not yet been defined. This study aimed to evaluate which parameters derived from CT, inflammatory, and hormonal markers could explain the clinical variability of COVID-19. We performed a retrospective study including SARS-CoV-2-infected patients hospitalized from March 2020 to May 2021 at the Umberto I Polyclinic of Rome. Patients were divided into four groups according to the degree of respiratory failure. Routine laboratory examinations, BMI, liver steatosis indices, liver CT attenuation, ferritin, and IGF-1 serum levels were assessed and correlated with severity. Analysis of variance between groups showed that patients with worse prognoses had higher BMI and ferritin levels, but lower liver density, albumin, GH, and IGF-1. ROC analysis confirmed the prognostic accuracy of IGF-1 in discriminating between patients who experienced death/severe respiratory failure and those who did not (AUC 0.688, CI: 0.587 to 0.789, <i>p</i> < 0.001). A multivariate analysis considering the degrees of severity of the disease as the dependent variable and ferritin, liver density, and the standard deviation score of IGF-1 as regressors showed that all three parameters were significant predictors. Ferritin, IGF-1, and liver steatosis account for the increased risk of poor prognosis in COVID-19 patients with obesity.
Fatal Air Embolism Associated With Pneumatosis Cystoides Intestinalis (2013)

We report a unique fatality associated with pneumatosis cystoides intestinalis. The proposed mechanism of lethality is air embolization. Our basis for this interpretation is autopsy exclusion of other lethal mechanisms in association with identifiable physical findings. We offer anatomic and physiologic explanations for our interpretation, in association with a brief discussion thereof.
Xenobiotic‐metabolizing Cytochromes P450 in Ontogeny: Evolving Perspective (2004)

While much is known about inducibility of the xenobiotic-metabolizing forms of cytochrome P450, the Family 1-3 enzymes, less well understood is the purpose for the presence of some of these forms in the developing conceptus. Many cytochrome P450 forms are present in the embryo and fetus, like the anabolic forms in Families 5 and higher, and are known to produce molecules with specific functions, e.g., cholesterol, steroids, and their metabolites necessary for normal physiological functions. As we gain greater understanding of the cell cycle and its regulation, and the roles of nuclear receptors in modulating transcriptional activities, a picture begins to emerge in which cytochrome P450 forms appear as molecule-altering enzymes producing and eliminating ligands associated with nuclear receptor activities. For these CYP enzymes to exert a developmental action, a controlled spatial and temporal expression pattern would be essential. Studies now indicate the existence of such temporal control on the appearance of a number of these enzymes and the necessary coenzyme, NADPH-cytochrome P450 reductase.
Infrared nanospectroscopic imaging in the rotating frame (2019)

Infrared (IR) vibrational scattering scanning near-field optical microscopy (s-SNOM) has advanced to become a powerful nanoimaging and spectroscopy technique with applications ranging from biological to quantum materials. However, full spatiospectral s-SNOM continues to be challenged by long measurement times and drift during the acquisition of large associated datasets. Here, we demonstrate a novel approach of computational spatiospectral s-SNOM by transforming the basis from the stationary frame into the rotating frame of the IR carrier frequency. We demonstrate an acceleration of IR s-SNOM data collection by a factor of 10 or more in combination with prior knowledge of the electronic or vibrational resonances to be probed, the IR source excitation spectrum, and other general sample characteristics. As an example, we apply rotating-frame s-SNOM (R-sSNOM) to chemical nanoimaging of ultrathin protein sheets in a mollusk shell. R-sSNOM enables high-voxel-density imaging of sparsely distributed molecules in an extended matrix. It is generally applicable to many multiscale material systems with sparse features and can be extended to other spectroscopic nanoimaging modalities.
A tissue atlas of ulcerative colitis to guide TNF inhibitor therapy (2021)

Abstract Ulcerative colitis is a chronic-relapsing inflammatory disease of the large intestine with a complex, multifactorial pathogenesis. TNF inhibitors are widely used to suppress immune-mediated tissue damage in ulcerative colitis patients; however, therapy failures are common. Predicting TNF inhibitor response requires an understanding of the architectural features that underlie mucosal inflammation and those responsible for resistance. Here, we used highly multiplexed immunofluorescence to uncover the spatially resolved tissue architectures underlying disease progression and treatment response in 42 tissue regions from 34 individuals. We created a tissue atlas and performed spatial analysis to identify cell-cell contacts and cellular neighborhoods. We observed that cellular functional states depend on cellular neighborhood and that a subset of inflammatory cell types and cellular neighborhoods in ulcerative colitis patients persisted even during treatment with TNF inhibitor, indicating resistant niches. A computer vision model, with no a priori assumptions regarding cellular architectural features, was able to predict TNF inhibitor resistance. This spatial model significantly outperformed classification models based on single-cell data alone. Our results demonstrate the value of a spatial tissue atlas as a precision medicine tool to guide treatment of patients suffering from autoimmune diseases.
The Immune Adherence Receptor: Dissociation Between the Expression of Erythrocyte and Mononuclear Cell C3b Receptors (1975)

Abstract Human erythrocytes have a surface receptor for the third component of complement (C3b). This receptor mediates the immune adherence (IA) reaction. In this study, erythrocytes from over 100 normal donors and from donors with rare blood group types were examined for IA reactivity. All donors had red cells with high titer IA activity, with the exception of one Rh null individual whose erythrocytes had barely detectable IA activity. Five other RH null donors had normal IA titers. Because of the apparent defect in the expression of the red cell C3b receptor in this one individual, the mononuclear cell C3b receptors of this donor were studied and found to be normal. These findings suggest that expression of the erythrocyte C3b receptor may be controlled by factors which differ from those determining expression of the mononuclear cell C3b receptor.
Atomic Layer Deposition of Titania in Ordered Mesoporous Cerium Zirconium Oxide Thin Films: A Case Study (2019)

A strategy is presented to deposit defined layers of TiO2 onto the pore surface within ordered mesoporous, crystalline CeO2-ZrO2 thin films using atomic layer deposition (ALD). As structure-directing agent, a special diblock copolymer, poly(isobutylene)-block-poly(ethylene oxide), was used, resulting in a three-dimensional arrangement of spherical pores with diameter around 13 nm. High resolution transmission electron microscopy investigations evidence the presence of anatase TiO2 coatings within the mesopores, while ellipsometric porosimetry studies together with time-of-flight secondary-ion mass spectrometry depth profiles indicate the deposition inside the mesopores up to 50 ALD cycles. Afterward, the interconnecting channels between the mesopores are filled completely prohibiting further transport of the gaseous TiO2 precursor into the ordered structure of mesopores and hence, limit the layer growth on the surfaces of the pores. Therefore, the size of the mesopores and their connections are decisive when growing transition-metal oxide layers on the surface of porous substrates and need to be considered for future depositions using ALD. The hybrid TiO2/CeO2-ZrO2 materials are studied by several complementary analytical techniques, to validate the deposition process and also the applicability of these techniques for such materials in general.
Image Representation by Complex Cell Responses (2004)

We present an analysis of the representation of images as the magnitudes of their transform with complex-valued Gabor wavelets. Such a representation is a model for complex cells in the early stage of visual processing and of high technical usefulness for image understanding, because it makes the representation insensitive to small local shifts. We show that if the images are band limited and of zero mean, then reconstruction from the magnitudes is unique up to the sign for almost all images.
BAPC-assisted CRISPR/Cas9 System: Targeted Delivery into Adult Ovaries for Heritable Germline Gene Editing (Arthropoda: Hemiptera) (2018)

ABSTRACT Innovative gene targeting strategies are often limited in application across arthropod species due to problems with successful delivery. In hemipterans, embryonic injections often used to deliver CRISPR components fail due to nearly complete embryo mortality. The Asian citrus psyllid, Diaphorina citri , Kuwayama, (Hemiptera: Liviidae), is the vector for a pathogenic bacterium, Candidatus Liberibacter asiaticus, CLas, which is devastating the U.S. citrus industries. The disease called, Huanglongbing, HLB, (aka. Citrus greening disease), is transmitted during psyllid feeding. Infection causes severe tree decline, loss of fruits, and eventually tree death. The citrus tree pathogen, CLas, is a fastidious alpha-proteobacterium, which has spread into all citrus growing regions worldwide. The economic losses are estimated in the billions of dollars, in U.S.A., Brazil, and China. Innovative technologies aimed at reducing psyllid populations using targeting RNA suppression, like RNAi, or gene-editing tools, like CRISPR/Cas9 have potential to reduce psyllid vectors and the pathogen in a highly specific manner. Breakthroughs that improve gene editing in psyllids, such as the BAPC-assisted -CRISPR/Cas9 System, enabled delivery by injection of CRISPR/Cas9 components directly into nymphs and adult females. Injection near ovaries produced heritable germline gene editing in subsequent generations. This method opens the world of gene editing across arthropods and bypasses the need for microinjection of eggs. Effective development of therapeutic treatments to reduce insect vectors, and stop pathogen transmission would provide sustainable citrus and grapevine industries.
CCR Translation for the Article from Synergistic Enhancement of CD8<sup>+</sup> T Cell–Mediated Tumor Vaccine Efficacy by an Anti–Transforming Growth Factor-β Monoclonal Antibody (2023)

CCR Translation for the Article from Synergistic Enhancement of CD8&lt;sup&gt;+&lt;/sup&gt; T Cell–Mediated Tumor Vaccine Efficacy by an Anti–Transforming Growth Factor-β Monoclonal Antibody