gebhart/patrepeval huggingface dataset:
Delivery of miR-424-5p via Extracellular Vesicles Promotes the Apoptosis of MDA-MB-231 TNBC Cells in the Tumor Microenvironment (2021)

Programmed cell death ligand-1 (PD-L1) overexpressed on cancer cells has emerged as a key inhibitor that maintains the immunosuppressive microenvironment through its interaction with the PD-1 receptor in cancer. Here, we demonstrated that miR-424-5p delivery via extracellular vesicles (EVs) derived from adipose tissue-mesenchymal stromal cells (AT-MSCs) partly promotes proinflammation and enhances antitumor cytotoxicity in vitro and in vivo. Triple negative breast cancer (TNBC) exhibits increased expression of <i>PD-L1</i>, and <i>PD-L1</i> is positively correlated with the overall survival of patients with TNBC. PD-L1 shows relatively higher expression in MDA-MB-231 (MM231) cells and can be downregulated by miR-424-5p. Furthermore, miR-424-5p transported by EVs can increase the secretion of proinflammatory cytokines, decrease the secretion of anti-inflammatory cytokines and promote the apoptosis of tumor cells. The intratumoral administration of miR-424-5p-EVs significantly slowed tumor growth. In conclusion, these results demonstrate that EVs may serve as a delivery system for novel immunotherapies for TNBC through the miR-424-5p/PD-L1 pathway.
Identification of Oxidation Products of (−)-Epigallocatechin Gallate and (−)-Epigallocatechin with H2O2 (2000)

(-)-Epigallocatechin gallate (EGCG) and (-)-epigallocatechin (EGC) are two important antioxidants in tea. They also display some antitumor activities, and these activities are believed to be mainly due to their antioxidative effects. However, the specific mechanisms of antioxidant action of tea catechins remain unclear. In this study are isolated and identified two novel reaction products of EGCG and one product of EGC when they were reacted separately with H(2)O(2). These products are formed by the oxidation and decarboxylation of the A ring in the catechin molecule. This study provides unequivocal proof that the A ring of EGCG and EGC may also be an antioxidant site. This study also indicates an additional reaction pathway for the oxidation chemistry of tea catechins.
Data Supplement from The lncRNA <i>PCAT29</i> Inhibits Oncogenic Phenotypes in Prostate Cancer (2023)

&lt;p&gt;Supplementary Figure 1. Gene sequence information of PCAT29.&lt;/p&gt;
Multicell-to-Multicell Equalizers Based on Matrix and Half-Bridge LC Converters for Series-Connected Battery Strings (2019)

Due to the low switching loss, compact size, and low price, the LC series resonant converter (LCSRC) as an energy carrier is promising in battery equalizers. However, the conventional equalizers based on LCSRC only can realize the one-cell-to-one-cell balancing for a high-voltage series-connected battery pack, causing a low equalizing speed and efficiency. Therefore, two direct multicell-to-multicell (MC2MC) balancing topologies based on matrix LC converter and half-bridge LC converter are proposed, respectively, which achieve the direct energy transfer from a consecutive more-charged-cell group to a consecutive less-charged-cell group, significantly improving the equalizing speed and efficiency. An analytical model for the LCSRC is developed to provide a guidance for the optimal design of the proposed equalizers. The prototypes for the proposed two balancing topologies are constructed. The MC2MC balancing performances are verified though the comparative experimental results at different initial voltage distributions. In particular, the proposed equalizers simultaneously achieve the fast balancing speed and high equalization efficiency. The measured peak efficiency is about 87.9% at the four-cell-to-four-cell equalization.
Correlation of stress-induced leakage current with generated positive trapped charges for ultrathin gate oxide (1998)

In this work, the evidence of the stress-induced leakage current related to the stress-generated positive trapped charges is presented and investigated. It is shown that the centroid of the positive trapped charges, which depends on the polarity of the stress current, affects the magnitude of the leakage current. The trapping density of positive charges, which is determined by the final stress applied on the oxide, determines the final level of the leakage currents.
Interfacial Structure of Phosphorylcholine Incorporated Biocompatible Polymer Films (2003)

Neutron reflectivity (NR) has been used to determine the structure of dry and swollen PC100B biocompatible phosphorylcholine (PC) polymer films coated on the optically flat silicon oxide surfaces. Deuterium labeling to the dodecyl chain of PC100B polymer was found to be effective at highlighting the interfacial structure of dry PC100B films while the swelling with D2O into the fully hydrogenated PC100B polymer produced sufficient isotopic contrast for revealing structural features within the swollen films. The main structural characteristics of the dry PC films were found to consist of alternate layers at the SiO2/polymer and polymer/air interfaces, indicating that thermal annealing promoted segregation of hydrophilic and hydrophobic moieties within the polymer. The thicknesses of these sublayers were between 10 and 30 Å, and the layering feature gradually became diminished into the middle film region which is characterized by a uniform scattering length density (SLD) determined by NR. It was further observed that the outer surfaces of the dry polymer films contained a significant fraction of hydrophobic moiety as a result of surface energy minimization during annealing, but upon swelling in water this structural feature was deteriorated by fragment motions and redistributions. In contrast to the greater structural variation on the outer film surface, swelling had relatively less effect on the internal layering structure at the SiO2/polymer interface due to the structural constraints imposed by silyl cross-linking and the hydrophobic barrier to water diffusion. The thickness of the middle part of uniform region increases with dry film thickness, but the alternate layering at the interfaces was largely unaffected. The increase in annealing temperature enhanced silyl cross-linking, resulting in the reduced equilibrium water content across the films and the slowdown of the rate of swelling.
High incidence of monoclonal EBV episomes in Hodgkin's disease and anaplastic large‐cell ki‐1‐positive lymphomas in HIV‐1‐positive patients (1993)

A series of selected lymphoid malignancies (LMs) occurring in Italian HIV-1-infected (HIV+) patients, principally intravenous drug users, was investigated. In addition to small non-cleaved-cell (SNCC) and large-cell immunoblastic (LCI) non-Hodgkin's lymphomas (NHLs), a relatively high occurrence of anaplastic large-cell Ki-I-positive (ALC Ki-I+) lymphomas and Hodgkin's disease (HD) was observed, at variance with other reported series of HIV+ patients. Combined results of in situ hybridization and Southern-blot analyses, in conjunction with immunohistochemical detection of Epstein-Barr virus (EBV)-encoded latent membrane protein-I (LMP-I), showed an almost complete association of ALC Ki-I+ lymphomas and HD cases with EBV. The neoplastic cells of both these LMs also showed common immunophenotypic features such as frequent absence of B- and T-cell differentiation markers and expression of the Ki-I activation marker, while SNCC and LCI lymphomas were mainly of mature B-cell origin and Ki-I-. The concomitant high incidence of ALC Ki-I+ lymphomas and HD in a specific group of HIV+ patients, their almost complete association with EBV in clonal and episomal form and the great similarity in differentiation, activation and virological markers which they display suggest that these LMs are pathological variants of a continuous spectrum of HIV-I-associated disorders etiopathologically linked to EBV.
Graft failure after T-cell-depleted human leukocyte antigen identical marrow transplants for leukemia: II. In vitro analyses of host effector mechanisms (1989)

To identify mechanisms potentially contributing to graft failure, 19 leukemic recipients of T-cell-depleted marrow transplants who failed to engraft following a transplant of HLA identical sibling marrow depleted of T cells by soybean agglutinin (SBA) and sheep erythrocytes (E) were evaluated. Peripheral blood mononuclear cells isolated at the time of failure were consistently of host origin, bearing the phenotype of suppressor T cells (CD3+, CD8+, Leu 7+). A direct cytolytic effect on 51Cr-labeled donor-derived target cells was not detected, a finding that contrasts with the donor-specific cytotoxic host T lymphocytes that have been regularly observed in patients rejecting HLA nonidentical SBA -E- BMTs. However, these host T cells did exhibit a strong and specific suppressive activity against the donor marrow CFU-GM in vitro. Furthermore, in contrast to prior findings in durably engrafted recipients of SBA -E- BMTs, the lymphocytes isolated prior to or at the time of graft failure lacked natural killer surface antigen expression and effector function.
HRTEM Studies of Morphological and Interfacial Changes of Nanodiamond in Field Emission Experiments (2002)

Journal Article HRTEM Studies of Morphological and Interfacial Changes of Nanodiamond in Field Emission Experiments Get access T Tyler, T Tyler Dept. Materials Science and Eng., N.C. State University, Raleigh, NC 27695-7907 Search for other works by this author on: Oxford Academic Google Scholar A V Kvit, A V Kvit Dept. Materials Science and Eng., N.C. State University, Raleigh, NC 27695-7907 Search for other works by this author on: Oxford Academic Google Scholar V V Zhirnov, V V Zhirnov Dept. Materials Science and Eng., N.C. State University, Raleigh, NC 27695-7907 Search for other works by this author on: Oxford Academic Google Scholar J J Hren J J Hren Dept. Materials Science and Eng., N.C. State University, Raleigh, NC 27695-7907 Search for other works by this author on: Oxford Academic Google Scholar Microscopy and Microanalysis, Volume 8, Issue S02, 1 August 2002, Pages 1110–1111, https://doi.org/10.1017/S143192760210362X Published: 01 November 2002
Electrostatic charge sensing using nanocavity-based Raman silicon laser (2021)

We investigated the behavior of a Raman silicon laser based on a photonic-crystal nanocavity while it was irradiated with electrostatic charges from a static-electricity generator. Laser oscillation stopped after a few seconds of a weak irradiation and the time needed for the recovery of laser oscillation increased with irradiation time. We measured the resonance spectra of the nanocavity modes during irradiation. It indicates that such a lasing interruption can be caused by wavelength shifts of the nanocavity modes and absorption losses due to the electrostatic charges on the nanocavity surface. These results are useful for the sensing of electrostatic charges.
Size of alkyl group R: principal factor determining wettability of surface-functionalized polyethylenes (PE-CONHR and PE-CO2R) by water (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSize of alkyl group R: principal factor determining wettability of surface-functionalized polyethylenes (PE-CONHR and PE-CO2R) by waterMark D. Wilson, Gregory S. Ferguson, and George M. WhitesidesCite this: J. Am. Chem. Soc. 1990, 112, 3, 1244–1245Publication Date (Print):January 1, 1990Publication History Published online1 May 2002Published inissue 1 January 1990https://pubs.acs.org/doi/10.1021/ja00159a053https://doi.org/10.1021/ja00159a053research-articleACS PublicationsRequest reuse permissionsArticle Views136Altmetric-Citations20LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Biomarkers of Tuberculosis Severity and Treatment Effect: A Directed Screen of 70 Host Markers in a Randomized Clinical Trial (2017)

More efficacious treatment regimens are needed for tuberculosis, however, drug development is impeded by a lack of reliable biomarkers of disease severity and of treatment effect. We conducted a directed screen of host biomarkers in participants enrolled in a tuberculosis clinical trial to address this need. Serum samples from 319 protocol-correct, culture-confirmed pulmonary tuberculosis patients treated under direct observation as part of an international, phase 2 trial were screened for 70 markers of infection, inflammation, and metabolism. Biomarker assays were specifically developed for this study and quantified using a novel, multiplexed electrochemiluminescence assay. We evaluated the association of biomarkers with baseline characteristics, as well as with detailed microbiologic data, using Bonferroni-adjusted, linear regression models. Across numerous analyses, seven proteins, SAA1, PCT, IL-1β, IL-6, CRP, PTX-3 and MMP-8, showed recurring strong associations with markers of baseline disease severity, smear grade and cavitation; were strongly modulated by tuberculosis treatment; and had responses that were greater for patients who culture-converted at 8weeks. With treatment, all proteins decreased, except for osteocalcin, MCP-1 and MCP-4, which significantly increased. Several previously reported putative tuberculosis-associated biomarkers (HOMX1, neopterin, and cathelicidin) were not significantly associated with treatment response. In conclusion, across a geographically diverse and large population of tuberculosis patients enrolled in a clinical trial, several previously reported putative biomarkers were not significantly associated with treatment response, however, seven proteins had recurring strong associations with baseline radiographic and microbiologic measures of disease severity, as well as with early treatment response, deserving additional study.
The application of a crosslinked pectin‐based wafer matrix for gradual buccal drug delivery (2012)

The purpose of this study was to develop crosslinked wafer matrices and establish the influence of the crosslinker type and processing sequence on achieving gradual buccal drug delivery. Three sets of drug-loaded crosslinked pectin wafers were produced employing the model water-soluble antihistamine, diphenhydramine and were compared with noncrosslinked wafers. The formulations were crosslinked with CaCl(2), BaCl(2), or ZnSO(4) pre- or postlyophilization (sets 1 and 2) as well as pre- and postlyophilization (set 3), respectively. The surface morphology, porositometry, molecular vibrational transitions, textural attributes, thermal and in vitro drug release were characterized and supported by in silico molecular mechanics simulations. Results revealed that crosslinked wafers produced smaller pore sizes (107.63 Å) compared with noncrosslinked matrices (180.53 Å) due to molecular crosslinks formed between pectin chains. Drug release performance was dependent on the wafer crosslinking production sequence. Noncrosslinked wafers displayed burst-release with 82% drug released at t(30min) compared with first-order kinetic profiles obtained for prelyophilized crosslinked matrices (50% released at t(30min) followed by steady release). Wafers crosslinked postlyophilization displayed superior control of drug release (40% at t(30min)). Molecular mechanics simulations corroborated with the experimental data and established that Ba(++), having the largest atomic radii (1.35 Å) formed a number of ionic bridges producing wafers of higher porosity (0.048 cm(2)/g) and had more influence on drug release.
Multi-Compartment Spatially-Derived Radiomics From Optical Coherence Tomography Predict Anti-VEGF Treatment Durability in Macular Edema Secondary to Retinal Vascular Disease: Preliminary Findings (2021)

This study revealed that the texture-based radiomics features pertaining to IRF subcompartment were most discriminating between rebounders and non-rebounders to anti-VEGF therapy. Clinical Impact: With further validation, OCT-based imaging biomarkers could be used for treatment management of DME patients.
Validity and reliability of the Health Assessment Questionnaire among patients with spondyloarthritis in Singapore (2016)

This study supports the HAQ as a valid and reliable measure of disability for use in patients with SpA.
Therapy-induced developmental reprogramming of prostate cancer cells and acquired therapy resistance (2017)

Treatment-induced neuroendocrine transdifferentiation (NEtD) complicates therapies for metastatic prostate cancer (PCa). Based on evidence that PCa cells can transdifferentiate to other neuroectodermally-derived cell lineages in vitro, we proposed that NEtD requires first an intermediary reprogramming to metastable cancer stem-like cells (CSCs) of a neural class and we demonstrate that several different AR+/PSA+ PCa cell lines were efficiently reprogrammed to, maintained and propagated as CSCs by growth in androgen-free neural/neural crest (N/NC) stem medium. Such reprogrammed cells lost features of prostate differentiation; gained features of N/NC stem cells and tumor-initiating potential; were resistant to androgen signaling inhibition; and acquired an invasive phenotype in vitro and in vivo. When placed back into serum-containing mediums, reprogrammed cells could be re-differentiated to N-/NC-derived cell lineages or return back to an AR+ prostate-like state. Once returned, the AR+ cells were resistant to androgen signaling inhibition. Acute androgen deprivation or anti-androgen treatment in serum-containing medium led to the transient appearance of a sub-population of cells with similar characteristics. Finally, a 132 gene signature derived from reprogrammed PCa cell lines distinguished tumors from PCa patients with adverse outcomes. This model may explain neural manifestations of PCa associated with lethal disease. The metastable nature of the reprogrammed stem-like PCa cells suggests that cycles of PCa cell reprogramming followed by re-differentiation may support disease progression and therapeutic resistance. The ability of a gene signature from reprogrammed PCa cells to identify tumors from patients with metastasis or PCa-specific mortality implies that developmental reprogramming is linked to aggressive tumor behaviors.
Data-Driven Completion of Motion Capture Data (2011)

We present a data-driven method for completion of corrupted marker-based motion capture data. Our novel approach is especially suitable for challenging cases, e.g. if complete marker sets of multiple body parts are missing over a long period of time. Without the need for extensive preprocessing we are able to fix missing markers across different actors and motion styles. Our approach can be used for incrementally increasing prior-databases, as the underlying search technique for similar motions scales well to huge databases.
Molecular evolutionary engineering of xylose isomerase to improve its catalytic activity and performance of micro-aerobic glucose/xylose co-fermentation in Saccharomyces cerevisiae (2019)

This study demonstrated that <i>Lp</i>XI has the highest d-xylose consumption rate among the XIs expressed in IR-2 under micro-aerobic co-fermentation conditions. A combination of novel mutations (T63I and V162A) significantly improved the enzymatic activity of <i>Lp</i>XI, indicating that <i>Lp</i>XI-<i>T63I/V162A</i> would be a potential construct for highly efficient production of cellulosic ethanol.
Cancer Invasion and Metastases (1990)

<h3>SELECTED CASE</h3> EIGHT years ago, a 62-year-old woman sought evaluation for an asymptomatic 2-cm mammographic abnormality in the upper part of the outer quadrant of her left breast. The results of an incisional biopsy were indicative of malignancy. After staging evaluation revealed no distant metastatic disease, she under-went a modified radical mastectomy with axillary node dissection. At the time of pathological review, a 2 × 2-cm estrogen receptor—positive infiltrating ductal adenocarcinoma was found; axillary lymph nodes were free of tumor (stage II, T2, NO, MO). Follow-up was uneventful until presentation 1 year ago, when the patientdeveloped progressive dyspnea on exertion, orthopnea, a dry cough, and a heaviness in the left side of her chest. At the time of examination, she was tachypneic and tachycardiac, and physical findings included dullness to percussion and absent left-sided breath sounds; she was admitted for treatment. A chest roentgenogram showed an opacified left hemithorax and
Clonal analysis of human T lymphocytes infiltrating the liver in chronic active hepatitis B and primary biliary cirrhosis (1988)

Human T lymphocytes infiltrating the liver in chronic active hepatitis B (CAH-B) and primary biliary cirrhosis were isolated from liver biopsy cores, cloned by limiting dilution technique and expanded in vitro. Phenotypic and functional analysis demonstrates that this tissue infiltrate represents a heterogeneous cell population. However, when compared to peripheral blood lymphocytes of the same patients, a marked enrichment for T8+ cytotoxic T cells was found to exist at a local site in both types of chronic liver disease. These data provide support for the notion that liver cell injury in CAH-B and PBC may be mediated by a common immunologic mechanism likely executed by cells of the T lineage.
Characterization and Correction of Symptomatic Hip Impingement in American Football Linemen (2018)

While cephalad retroversion was observed in approximately half of the hips, a significant cam deformity was seen maximally at 12:45, a more posterior cam location than that of the general population. Managing this pathology required obtaining preoperative and intraoperative images to characterize lesions and allow for their complete correction. With complete correction of the deformity, simulated range of motion demonstrated significant improvement in flexion, IR90, and FADIR maneuvers.
Effect of MR truncation compensation on quantitative PET image reconstruction for whole-body PET/MR (2011)

Truncation compensation (TC) is critical in MR based attenuation correction of quantitative PET image reconstruction in clinical whole-body PET/MR systems. The purpose of this study is to quantitatively evaluate the impact of MR image truncation on PET image reconstruction and the performance of a newly developed TC technique, using Monte-Carlo (MC) simulation. The NCAT phantom was used to create voxelized activities for various organs and hot lesions, and the corresponding attenuation maps (AMs), for a typical FDG patient study. The PURE simulator, which models the Philips Gemini TF PET system, was used to generate realistic list mode data. The full AM with arms down was cropped to create the truncated AM based on the MR field of view. The TC technique was applied on the truncated AM and the corresponding non-attenuation corrected reconstructed image to create a truncation-compensated AM. The list mode data was reconstructed using the above three AMs, and lesion activities were extracted from the reconstructed images. Using the lesion activities from the full AM as the ground truth, errors of the estimated lesion activities from the truncated AM and the truncation-compensated AM were calculated. The study demonstrated that truncation of the MR image based AM considerably affected lesion activity estimation from reconstructed PET images. Our TC technique effectively reduced errors of lesion activity estimation caused by the truncation.