gebhart/patrepeval huggingface dataset:
HTLV-1 Extracellular Vesicles Promote Cell-to-Cell Contact (2019)

Human T-cell leukemia virus-1 (HTLV-1) is a neglected and incurable retrovirus estimated to infect 5 to 10 million worldwide. Specific indigenous Australian populations report infection rates of more than 40%, suggesting a potential evolution of the virus with global implications. HTLV-1 causes adult T-cell leukemia/lymphoma (ATLL), and a neurological disease named HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP). Even though HTLV-1 transmission primarily occurs from cell-to-cell, there is still a gap of knowledge regarding the mechanisms of viral spread and disease progression. We have recently shown that Extracellular Vesicles (EVs) ubiquitously produced by cells may be used by HTLV-1 to transport viral proteins and RNA, and elicit adverse effects on recipient uninfected cells. The viral proteins Tax and HBZ are involved in disease progression and impairment of autophagy in infected cells. Here, we show that activation of HTLV-1 via ionizing radiation (IR) causes a significant increase of intracellular Tax, but not EV-associated Tax. Also, lower density EVs from HTLV-1-infected cells, separated by an Iodixanol density gradient, are positive for gp61+++/Tax+++/HBZ+ proteins (HTLV-1 EVs). We found that HTLV-1 EVs are not infectious when tested in multiple cell lines. However, these EVs promote cell-to-cell contact of uninfected cells, a phenotype which was enhanced with IR, potentially promoting viral spread. We treated humanized NOG mice with HTLV-1 EVs prior to infection and observed an increase in viral RNA synthesis in mice compared to control (EVs from uninfected cells). Proviral DNA levels were also quantified in blood, lung, spleen, liver, and brain post-treatment with HTLV-1 EVs, and we observed a consistent increase in viral DNA levels across all tissues, especially the brain. Finally, we show direct implications of EVs in viral spread and disease progression and suggest a two-step model of infection including the release of EVs from donor cells and recruitment of recipient cells as well as an increase in recipient cell-to-cell contact promoting viral spread.
Correction to MoS2 Field-Effect Transistor for Next-Generation Label-Free Biosensors (2014)

ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionORIGINAL ARTICLEThis notice is a correctionCorrection to MoS2 Field-Effect Transistor for Next-Generation Label-Free BiosensorsDeblina Sarkar*, Wei Liu, Xuejun Xie, Aaron C. Anselmo, Samir Mitragotri, and Kaustav Banerjee*Cite this: ACS Nano 2014, 8, 5, 5367Publication Date (Web):May 2, 2014Publication History Received24 April 2014Published online2 May 2014Published inissue 27 May 2014https://pubs.acs.org/doi/10.1021/nn502216dhttps://doi.org/10.1021/nn502216dcorrectionACS PublicationsCopyright © 2014 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views2410Altmetric-Citations7LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (50 KB) Get e-AlertscloseSUBJECTS:Biotechnology,Mass spectrometry,Sensors,Transistors Get e-Alerts
Increased detection of HIV-specific T cell responses by combination of central sequences with comparable immunogenicity (2008)

All tested centralized antigens provided a similarly potent set of antigenic peptides. However, the significantly broader responses detected using the combination of sets highlight the importance of maximizing coverage of HIV-1 sequence diversity in vaccine preparations, as well as in the evaluation of CTL responses in HIV-1-infected individuals and those vaccinated.
Functional interaction of heat shock protein GroEL with an RNase E-like activity in Escherichia coli. (1993)

The highly specific endoribonuclease activities of RNase E (which processes ribosomal 9S RNA into p5S RNA) and RNase K (which initiates decay of the ompA mRNA) are inferred to play a central role in RNA processing and mRNA decay in Escherichia coli. In vivo both activities are affected by a conditional mutation of the ams/rne gene that seems to be complemented at nonpermissive temperatures by a fragment of the groEL gene. Analysis of the relationship between the two nucleases and the heat shock protein revealed that GroEL interacts functionally with an RNase E-like activity but not with an RNase K activity, a groEL mutation affected 9S RNA processing but not ompA mRNA cleavage, RNase E activity could be precipitated with an antibody against GroEL, and a highly purified GroEL preparation contained RNase E activity but not RNase K activity. When purifying RNase E activity, we obtained a preparation containing two major proteins of 60 and 17 kDa. The size and the N-terminal sequence identified the 60-kDa protein as GroEL.
Role of theBcl-2 Oncogene in Hodgkin’s Disease (1994)

Journal Article Role of the Bcl-2 Oncogene in Hodgkin’s Disease Giorgio Inghirami, MD, Giorgio Inghirami, MD Hemalopathology/Molecular Pathology Section Department of Pathology New York University New York, New York Search for other works by this author on: Oxford Academic Google Scholar Glauco Frizzera, MD Glauco Frizzera, MD Hemalopathology/Molecular Pathology Section Department of Pathology New York University New York, New York Search for other works by this author on: Oxford Academic Google Scholar American Journal of Clinical Pathology, Volume 101, Issue 6, 1 June 1994, Pages 681–683, https://doi.org/10.1093/ajcp/101.6.681 Published: 01 June 1994
Copolymerization of Styrene and Ethylene at High Temperature with Titanocenes Containing a Pendant Amine Donor (2008)

The copolymerization of ethylene and styrene at 120 °C was investigated with a series of titanocenes: Cp′TiCl2L [1, 2: Cp′ = cyclopentadienyl (Cp) versus 3, 4: Cp′ = {2-(dimethylamino)ethyl}cyclopentadienyl (CpN); 1, 3: L = Cl versus 2, 4: L = 2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO)], in combination with methylaluminoxane (MAO). At 120 °C, titanocenes bearing a pendant amine ligand (3 and 4) are effective catalysts for the ethylene-styrene (ES) copolymerization, whereas complexes lacking the pendant group (1 and 2) afford only mixtures of polyethylene (PE) and atactic polystyrene (aPS). At these temperatures, copolymerization with complexes 3 and 4 yields mixtures of ES copolymers and atactic polystyrene that are not readily separated by solvent extraction or gel permeation chromatography as both components of the mixture exhibit similar molecular weights and solubility in THF and acetone. The source of the atactic polystyrene was the autopolymerization of styrene as deduced by carrying out the copolymerization in the presence of a catalytic chain transfer agent, cobalt tetraphenylporphyrin (Co(tpp)). The addition of Co(tpp) caused a decrease in the molecular weight of the atactic polystyrene but had no effect on the molecular weight of the ES copolymer.
The Influence of Protein Structure on the Products Emerging from Succinimide Hydrolysis (2002)

Proteins are vulnerable to spontaneous, covalent modifications that may result in alterations to structure and function. Asparagines are particularly labile, able to undergo deamidation through the formation of a succinimide intermediate to produce either aspartate or isoaspartate residues. Although aspartates cannot undergo deamidation they can form a succinimide and result in the same products. Isoaspartyls are the principal product of succinimide hydrolysis, accounting for 65-85% of the emerging residues. The variability in the ratio of products emerging from succinimide hydrolysis suggests the ability of protein structure to influence succinimide outcome. In the H15D histidine-containing protein (HPr), phosphorylation of the active site aspartate catalyzes the formation of a cyclic intermediate. Resolution of this species is exclusively to aspartate residues, suggestive of either a succinimide with restrained hydrolysis, or an isoimide, from which aspartyl residues are the only possible product. Deletion of the C-terminal residue of this protein does not influence the ability for phosphorylation or ring formation, but it does allow for isoaspartyl formation, verifying a succinimide as the cyclic intermediate in H15D HPr. Isoaspartyl formation in H15D Delta85 is rationalized to occur as a consequence of elimination of steric restrictions imposed by the C terminus on the main-chain carbonyl of the succinimide, the required point of nucleophilic attack of a water molecule for isoaspartyl formation. This is the first reported demonstration of the influence of protein structure on the products emerging from succinimide hydrolysis.
Discovery, Process Development, and Scale-Up of a Benzoxazepine-Containing mTor Inhibitor (2018)

A family of novel, highly potent, and selective inhibitors of the mammalian target of rapamycin (mTOR) containing the benzoxazepine core were identified. The lead compound (XL388), which exhibited low-nanomolar activity and >1000-fold selectivity over related PI3K kinases, was chosen for further development as a potential oral treatment of several different tumor types. It inhibited cellular phosphorylation of substrates of mTOR complexes 1 and 2. It also showed good pharmacokinetics, moderate oral bioavailability, and dose-dependent antitumor activity in mice implanted with human breast and colon tumor xenografts. The synthesis of the clinical candidate was rapidly scaled up to enable first-in-human studies. A safe and scalable route and process were developed for the tetrahydrobenzo[f][1,4]oxazepine core fragment. The constituent fragments of XL388 were assembled using a telescoped sequence to manufacture 7.8 kg of active pharmaceutical ingredient under current Good Manufacturing Practice (cGMP), in 99.7 HPLC area % purity and a 21% overall yield over eight synthetic steps.
Anti-acetylcholine receptor antibodies directed against the alpha-bungarotoxin binding site induce a unique form of experimental myasthenia. (1983)

Of seven rat monoclonal antibodies directed against nicotinic acetylcholine receptor, the three whose binding was blocked by alpha-bungarotoxin produced acute paralysis in chicken hatchlings, whereas the four others had no effect. In the affected animals, weakness and decremental electromyographic responses appeared within 1 hr after intravenous injection and both abnormalities improved after anticholinesterase administration. No histological changes were seen in the muscle of injected animals. These data suggest that antibodies binding in relationship to the cholinergic binding site, and presumably producing pharmacologic blockade of acetylcholine receptor, may play an important role in the pathogenesis of the defective neuromuscular transmission in myasthenia gravis.
ChemInform Abstract: Conductive Salts of Tropylium Ions with Tetracyanoquinodimethan Anion Radical (TCNQ·‐) and Bisthiadiazolotetracyanoquinodimethan Anion Radical (BTDA‐TCNQ·‐). (1988)

Abstract A wide range of 18 monocyclic tropylium cations (such as (III) and (VI) which are new compounds) and the bi‐ and tricyclic analogues (IX) and (X) are prepared and their reduction potentials are measured.
Polymer particles armored with cobalt oxide nanosheets for the catalytic degradation of bisphenol A (2022)

2D particle surfactants are attractive for the formation of highly stable emulsions and use as templates to prepare composite structures with performance properties dependent on the composition.
Do sugar residues contribute to the antigenic determinants responsible for protection and/or abolition of protection in Leishmania-infected BALB/c mice? (1986)

Intravenous inoculation of irradiated virulent promastigotes of Leishmania mexicana, or intradermal inoculation of avirulent temperature-sensitive clones of the same parasite, can protect BALB/c mice from progressive disease when challenged with parental organisms. However, if animals are prechallenged s.c. with irradiated parental parasites before (or shortly after, i.e., within 10 days) any other immunization regime is used, the s.c. challenge effectively suppresses development of protective immunity. The role of N-linked sugars on promastigotes in providing the determinants responsible for protection and/or suppression of protection in these assays has been examined. Growth of parasite in 2 micrograms/ml tunicamycin has no effect on incorporation of [3H]leucine but decreases incorporation of [3H]mannose by some 40 to 50%. Such tunicamycin-treated avirulent clones or irradiated virulent organisms are now unable to induce a protective response. However, tunicamycin-treated parental parasites given s.c. could still suppress the protection seen when irradiated parasites were given i.v. as immunogen. Tunicamycin-treated avirulent organisms given s.c. subcutaneously unlike the untreated avirulent clones, now also caused suppression of protection. These data suggest that the determinants responsible for development of protective immunity to L. mexicana in BALB/c mice are dependent on N-linked glycoproteins for their expression, unlike the determinants responsible for suppression of that protection. By using swainsonine as an alternative inhibitor of N-linked glycoprotein synthesis, the data additionally suggest that no complex processing of N-linked sugars takes place to reveal the immunogenic determinants in this system.
FCS UGV safe operations (2008)

The US Army Future Combat System (FCS) will implement Unmanned Ground Vehicles (UGV) in numbers not previously seen before in military operations. Many of these vehicles will also be larger and faster than the small robots typically used today for explosive ordnance disposal and general improvised explosive device handling. More importantly, FCS will implement these UGV's in scenarios were they will be in much closer proximity to soldiers and other non-combatant personnel. This paper describes the plan for developing an appropriate match of technology for autonomous UGV maneuver with the emerging need for safety release verification for these systems prior to fielding. The plan is followed by descriptions of initial data collections with a UGV, that will form the starting point in this safety release process, and stimulate further use and refinement of this process for large UGV's in applications beyond FCS.
ChemInform Abstract: A NOVEL APPROACH TO PHOSPHOPEPTIDE SYNTHESIS ‐ PREPARATION OF GLU‐PSER‐LEU (1984)

Abstract Aus dem N‐geschützten Serin (Ia) erhält man über den p‐Nitrobenzylester (Ib) den Phosphorsäureester (IIa), der durch katalytische Hydrierung zu (IIb) gespalten werden kann.
Structures of the two homologous series of dialkene mycolic acids from Mycobacterium smegmatis. (1982)

The positions of localization of the single carbon-carbon double bond in homologs of C series mycolic acids from Mycobacterium smegmatis have been established by 1) combined ammonia chemical ionization mass spectrometry/gas-liquid chromatography of aldehyde ozonolysis products, and 2) high resolution electron impact mass spectral analysis of vicinal glycol derivatives as their trimethylsilyl ethers. These studies have revealed that each homolog is a mixture of two isomers differing in double bond location. In each of the three homologs examined, approximately equal amounts of two isomers were present as follows: (formula: see text).
Blinatumomab Prior to CAR-T Cell Therapy—A Treatment Option Worth Consideration for High Disease Burden (2022)

The optimal bridging therapy before CAR-T cell infusion in pediatric relapsed or refractory B-cell precursor acute lymphoblastic leukemia (r/r BCP-ALL) still remains an open question. The administration of blinatumomab prior to CAR-T therapy is controversial since a potential loss of CD19+ target cells may negatively impact the activation, persistence, and, as a consequence, the efficacy of subsequently used CAR-T cells. Here, we report a single-center experience in seven children with chemorefractory BCP-ALL treated with blinatumomab before CAR-T cell therapy either to reduce disease burden before apheresis (six patients) or as a bridging therapy (two patients). All patients responded to blinatumomab except one. At the time of CAR-T cell infusion, all patients were in cytological complete remission (CR). Four patients had low positive PCR-MRD, and the remaining three were MRD-negative. All patients remained in CR at day +28 after CAR-T infusion, and six out of seven patients were MRD-negative. With a median follow-up of 497 days, four patients remain in CR and MRD-negative. Three children relapsed with CD19 negative disease: two of them died, and one, who previously did not respond to blinatumomab, was successfully rescued by stem cell transplant. To conclude, blinatumomab can effectively lower disease burden with fewer side effects than standard chemotherapeutics. Therefore, it may be a valid option for patients with high-disease burden prior to CAR-T cell therapy without clear evidence of compromising efficacy; however, further investigations are necessary.
Advanced Task Realization of Multi-Robot Systems by Interaction Mode Control (2007)

This paper proposes a unified control approach for multi-robot systems by interaction mode control. The proposed interaction mode control considers only the interactions between the systems. The interactions are abstracted by using the quarry matrix. Since the transformed modes are independent to each other, it is possible to design controller in the modal space.This paper also proposes a novel control index named “hybrid ratio”. The hybrid ratio is defined as the influence of the external acceleration input to the acceleration response of the system. Since it is possible to realize the assigned hybrid ratio in each mode according to the task, the motion command with the hybrid ratio is represented as the task code. Furthermore, the task code is able to be changed during the motion. Thus, the interaction mode control is treated as the task kinematics. As a result, intuitive and flexible controller design is attained by the proposed method.The proposed interaction mode control is applied for cooperative transportation by multi-robot systems. The experimental results show viability of the proposed method.
Syntheses of 6-Sulfo Sialyl Lewis X Glycans Corresponding to the l-Selectin Ligand “Sulfoadhesin” (2004)

[structure: see text] Divergent syntheses of sulfated sialyl Lewis X oligosaccharides corresponding to the core 1 and core 6 branches of the L-selectin ligand are reported. These synthetic targets incorporate a selectively protected serine residue at the reducing terminus, providing a functional handle for further conjugation.
Tris(1,10-phenanthroline-N,N′)iron(II) dithiocyanate trihydrate (2000)

The title mononuclear iron(II) complex, [Fe(C(12)H(8)N(2))(3)](NCS)(2).3H(2)O, has a slightly distorted octahedral coordination. One of the thiocyanate ions and one of the water molecules of crystallization show positional disorder.
Use of Non-distractive Testing AU-E Technology to Evaluate Hearth Conditions at CherMK–SEVERSTAL (2018)

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Numerical decomposition of symmetric linear systems (2015)

This paper proposes a method for numerically decomposing symmetric linear systems. We define system symmetries as transformations of the inputs, outputs, and states that do not change the system behavior. We show that symmetric systems can be decomposed into decoupled subsystems. We provide an algorithm for performing this decomposition that uses the input-output symmetries and minimal realizations to calculate the decomposition.
Detoxification of bilirubin and bile acids with intermittent coupled plasmafiltration and adsorption in liver failure (HERCOLE study) (2020)

CPFA resulted to be effective in liver detoxification. Thus, it may be considered as a "bridge technique" both to the liver transplant and to the recovery of the basal liver function.
Order Matters: Transmission Reordering in Wireless Networks (2011)

Modern wireless interfaces support a physical-layer capability called Message in Message (MIM). Briefly, MIM allows a receiver to disengage from an ongoing reception and engage onto a stronger incoming signal. Links that otherwise conflict with each other can be made concurrent with MIM. However, the concurrency is not immediate and can be achieved only if conflicting links begin transmission in a specific order. The importance of link order is new in wireless research, motivating MIM-aware revisions to link-scheduling protocols. This paper identifies the opportunity in MIM-aware reordering, characterizes the optimal improvement in throughput, and designs a link-layer protocol for enterprise wireless LANs to achieve it. Testbed and simulation results confirm the performance gains of the proposed system.
Automated acquisition of vast numbers of electron holograms with atomic-scale phase information (2020)

An automated acquisition system for collecting a large number of electron holograms, to improve the statistical precision of phase analysis, was developed. A technique for shifting the electron beam in combination with stage movement allows data to be acquired over a wide area of a TEM-specimen grid. Undesired drift in the hologram position, which may occur during the hologram acquisition, can be corrected in real time by automated detection of the interference-fringe region in an image. To demonstrate the usefulness of the developed automated hologram acquisition system, gold nanoparticles dispersed on a carbon foil were observed with a 1.2-MV atomic resolution holography electron microscope. The system could obtain 1024 holograms, which provided phase maps for more than 500 nanoparticles with a lateral resolution of 0.14 nm, in just 1 h. The observation results revealed an anomalous increase in mean inner potential for a particle size smaller than 4 nm. The developed automated hologram acquisition system can be applied to improve the precision of phase measurement by averaging many phase images, as demonstrated by single particle analysis for biological entities. Moreover, the system makes it possible to study electrostatic potential of catalysts and other functional nanoparticles at atomic resolution.