gebhart/patrepeval huggingface dataset:
Growth-inhibitory effects of DL-alpha-difluoromethylornithine in the spectrum of human lung carcinoma cells in culture. (1982)

dl-α-Difluoromethyl ornithine (DFMO) is a new and specific inhibitor of the first step in polyamine biosynthesis. We recently reported that DFMO profoundly diminishes cell survival in a single culture line of human small-cell lung carcinoma (SCC). In the present study, we have extended this work to ascertain: ( a ) the effects of DFMO on the spectrum of cell growth for SCC in culture; and ( b ) how the response of SCC cells to DFMO compares to that for the other three major types of human lung cancer cells. SCC cells were found to have a unique response to DFMO among the types of lung cancer; exposure of eight separate lines to the drug, during the exponential growth phase, produced an initial inhibition of cell increase followed by a progressive and complete loss of cells from the cultures. There was a heterogeneous response pattern among the SCC lines in that the time of onset for cell loss ranged from 4 days to 4 weeks. In marked contrast to SCC, two lines of human lung adenocarcinoma, one of squamous cell carcinoma and one of large cell undifferentiated carcinoma, had a more usual response to inhibition of polyamine biosynthesis. Despite a cessation of cell proliferation during DFMO treatment, no cell loss ensued over periods up to 8 weeks in these cultures. The above results with SCC and non-SCC cells suggested that the former could not live well in culture as a resting, nonproliferative cell. We further tested this hypothesis by exposing to DFMO stationary-phase 4-week-old aggregates of cultured SCC cells, in which cell proliferation and cell loss are balanced; within 2 weeks, the profound loss of cells was again manifest. We conclude that: ( a ) in culture, SCC cells, among human lung cancer types, have a unique sensitivity to inhibition of polyamine biosynthesis; ( b ) the heterogeneity for timing of SCC response to DFMO may have to be taken into account when investigating the therapeutic potential of the drug, DFMO; and ( c ) the biological differences between SCC and non-SCC cell in response to DFMO may provide a model system for establishing further the functional role of the polyamines.
AMP-18 Targets p21 to Maintain Epithelial Homeostasis (2015)

Dysregulated homeostasis of epithelial cells resulting in disruption of mucosal barrier function is an important pathogenic mechanism in inflammatory bowel diseases (IBD). We have characterized a novel gastric protein, Antrum Mucosal Protein (AMP)-18, that has pleiotropic properties; it is mitogenic, anti-apoptotic and can stimulate formation of tight junctions. A 21-mer synthetic peptide derived from AMP-18 exhibits the same biological functions as the full-length protein and is an effective therapeutic agent in mouse models of IBD. In this study we set out to characterize therapeutic mechanisms and identify molecular targets by which AMP-18 maintains and restores disrupted epithelial homeostasis in cultured intestinal epithelial cells and a mouse model of IBD. Tumor necrosis factor (TNF)-α, a pro-inflammatory cytokine known to mediate gastrointestinal (GI) mucosal injury in IBD, was used to induce intestinal epithelial cell injury, and study the effects of AMP-18 on apoptosis and the cell cycle. An apoptosis array used to search for targets of AMP-18 in cells exposed to TNF-α identified the cyclin-dependent kinase inhibitor p21 WAF1/CIP1. Treatment with AMP-18 blunted increases in p21 expression and apoptosis, while reversing disturbed cell cycle kinetics induced by TNF-α. AMP-18 appears to act through PI3K/AKT pathways to increase p21 phosphorylation, thereby reducing its nuclear accumulation to overcome the antiproliferative effects of TNF-α. In vitamin D receptor-deficient mice with TNBS-induced IBD, the observed increase in p21 expression in colonic epithelial cells was suppressed by treatment with AMP peptide. The results indicate that AMP-18 can maintain and/or restore the homeostatic balance between proliferation and apoptosis in intestinal epithelial cells to protect and repair mucosal barrier homeostasis and function, suggesting a therapeutic role in IBD.
Data from Deletion of Chromosome 11q Predicts Response to Anthracycline-Based Chemotherapy in Early Breast Cancer (2023)

&lt;div&gt;Abstract&lt;p&gt;Despite the recent consensus on the eligibility of adjuvant systemic therapy in patients with lymph node–negative breast cancer (NNBC) based on clinicopathologic criteria, specific biological markers are needed to predict sensitivity to the different available therapeutic options. We examined the feasibility of developing a genomic predictor of chemotherapy response and recurrence risk in 185 patients with NNBC using assembled arrays containing 2,460 bacterial artificial chromosome clones for scanning the genome for DNA copy number changes. After surgery, 90 patients received anthracycline-based chemotherapy, whereas 95 did not. Tamoxifen was administered to patients with hormone receptor–positive tumors. The association of genomic and clinicopathologic data and outcome was computed using Cox proportional hazard models and multiple testing adjustment procedures. Analysis of NNBC genomes revealed a common genomic signature. Specific DNA copy number aberrations were associated with hormonal receptor status, but not with other clinicopathologic variables. In patients treated with chemotherapy, none of the genomic changes were significantly correlated with recurrence. In patients not receiving chemotherapy, deletion of eight bacterial artificial chromosome clones clustered to chromosome 11q was independently associated with relapse (disease-free survival at 10 years ± SE, 40% ± 14% versus 86% ± 6%; &lt;i&gt;P&lt;/i&gt; &lt; 0.0001). The 54 patients with deletion of 11q (29%) did not present more aggressive clinicopathologic features than those without 11q loss. The adverse influence of 11q deletion on clinical outcome was confirmed in an independent validation series of 88 patients with NNBC. Our data suggests that patients with NNBC with the 11q deletion might benefit from anthracycline-based chemotherapy despite other clinical, pathologic, or genetic features. However, these initial findings should be evaluated in randomized clinical trials. [Cancer Res 2007;67(2):818–26]&lt;/p&gt;&lt;/div&gt;
Faculty Opinions recommendation of Palmitoylome profiling reveals S-palmitoylation-dependent antiviral activity of IFITM3. (2010)

Identification of immune effectors and the post-translational modifications that control their activity is essential for dissecting mechanisms of immunity. Here we demonstrate that the antiviral activity of interferon-induced transmembrane protein 3 (IFITM3) is post-translationally regulated by S-palmitoylation. Large-scale profiling of palmitoylated proteins in a dendritic cell line using a chemical reporter strategy revealed over 150 lipid-modified proteins with diverse cellular functions, including innate immunity. We discovered that S-palmitoylation of IFITM3 on membrane-proximal cysteines controls its clustering in membrane compartments and its antiviral activity against influenza virus. The sites of S-palmitoylation are highly conserved among the IFITM family of proteins in vertebrates, which suggests that S-palmitoylation of these immune effectors may be an ancient post-translational modification that is crucial for host resistance to viral infections. The S-palmitoylation and clustering of IFITM3 will be important for elucidating its mechanism of action and for the design of antiviral therapeutics. PMID: 20601941 Funding information This work was supported by: NIAID NIH HHS, United States Grant ID: AI083284-01 NIDA NIH HHS, United States Grant ID: R21 DA025751-02 NIDA NIH HHS, United States Grant ID: R21 DA025751 NIAID NIH HHS, United States Grant ID: R01 AI083284 NIDA NIH HHS, United States Grant ID: 1R21DA025751-01 NIAID NIH HHS, United States Grant ID: AI041111 NIAID NIH HHS, United States Grant ID: R01 AI041111 More Less keyboard_arrow_down
Surface Photovoltage Measurement of Hydrogen‐Treated Si Surfaces (1999)

A contactless surface photovoltage technique was used to measure surface‐potential barriers resulting from hydrogen termination of silicon surfaces and their evolution as the surfaces gradually oxidized in air at room temperature. Hydrogen termination formed by annealing in a hydrogen ambient was more complete than passivation formed by aqueous HF treatment. In addition, the surface‐potential barrier depended on the wafer orientation and ordering of surface terraces by high temperature hydrogen annealing. Oxides grown at room temperature exhibited higher surface‐potential barriers than oxides grown at elevated temperatures. © 1999 The Electrochemical Society. All rights reserved.
Discovery signal design and measurements for small cell DTX in Release 12 LTE (2014)

This paper examines an LTE Release 12 discovery signal design and proposes various enhancements for current measurement definitions to more accurately capture the interference level of a frequency layer performing discontinuous transmission (DTX). We evaluated accuracy of the proposed layer selection metric by link-level tools, showed system-level evaluations for a system using a small cell ON/OFF scheme with a discovery signal, and compared the benefits of proposed measurement enhancements against the current system. The results show that considerable cell-edge performance improvements can be achieved with a simple improvement in the frequency layer selection.
Novel approach for synthesizing Ge fine particles embedded in glass by ion implantation: Formation of Ge nanocrystal in SiO2-GeO2 glasses by proton implantation (1994)

Nanometer-sized crystalline Ge colloid particles have been formed by implantation of protons into substrate glasses having a composition of 1 GeO2-9SiO2 to a fluence of 1×1018 cm−2 at an energy of 1.5 MeV at room temperature without post-thermal annealing. Intensities of the absorption band due to Ge particles reach a maximum at ∼30 μm from the surface and their depth profile is close to that of the electronic energy loss. No formation of Si particles was observed in SiO2 or SiO2:GeO2 glasses implanted with protons at the same conditions and fluence.
Using aspects and dynamic composition to provide context-aware adaptation for mobile applications (2009)

Mobile systems characterize by dynamic environments, thus requiring adaptive and context aware mechanisms to perceive changes in the execution context and to dynamically adapt to them. We propose a framework for developing adaptive context aware applications which employs aspect-oriented techniques and dynamic composition to modularize the adaptive behavior and to keep apart the application logic from this behavior.
Lipoprotein(a) is a Therapeutic Target for Cardiovascular Disease (2012)

Lipoprotein(a) [Lp(a)] is known as an independent risk factor for cardiovascular diseases (CVD). Lp(a) is a unique lipoprotein consisting of the glycoprotein apolipoprotein(a) [apo(a)] covalently linked to apolipoproteinB-100 (apoB) in low-density lipoprotein (LDL) by a single disulfide bond. Although Lp(a) level is hugely variable and under strict genetic control, largely by apo(a) gene with kringle-4 type2 repeats, elevated level of plasma Lp(a) is correlated with CVD. Smaller isoforms with fewer kringle-4 repeats are associated with higher plasma Lp(a) level, leading to an increased risk for CVD. Lipid lowering agents (i.e. statins) have little or no effect on plasma Lp(a) level. Although it was reported that administration of niacin or estrogen may reduce the Lp(a) levels, there are no specific agents for the reduction of plasma Lp(a). In this review, we put together recent evidence and describe the overview of Lp(a) with the future direction. Keywords: Apolipoprotein(a), apolipoproteinB-100, atherosclerosis, cardiovascular diseases, coronary heart disease, kringle repeat, lipoprotein(a), plasminogen, risk factor
Comparison of the Depot Effect and Immunogenicity of Liposomes Based on Dimethyldioctadecylammonium (DDA), 3β-[N-(N′,N′-Dimethylaminoethane)carbomyl] Cholesterol (DC-Chol), and 1,2-Dioleoyl-3-trimethylammonium Propane (DOTAP): Prolonged Liposome Retention Mediates Stronger Th1 Responses (2010)

The immunostimulatory capacities of cationic liposomes are well-documented and are attributed both to inherent immunogenicity of the cationic lipid and more physical capacities such as the formation of antigen depots and antigen delivery. Very few studies have however been conducted comparing the immunostimulatory capacities of different cationic lipids. In the present study we therefore chose to investigate three of the most well-known cationic liposome-forming lipids as potential adjuvants for protein subunit vaccines. The ability of 3β-[N-(N',N'-dimethylaminoethane)carbomyl] cholesterol (DC-Chol), 1,2-dioleoyl-3-trimethylammonium propane (DOTAP), and dimethyldioctadecylammonium (DDA) liposomes incorporating immunomodulating trehalose dibehenate (TDB) to form an antigen depot at the site of injection (SOI) and to induce immunological recall responses against coadministered tuberculosis vaccine antigen Ag85B-ESAT-6 are reported. Furthermore, physical characterization of the liposomes is presented. Our results suggest that liposome composition plays an important role in vaccine retention at the SOI and the ability to enable the immune system to induce a vaccine specific recall response. While all three cationic liposomes facilitated increased antigen presentation by antigen presenting cells, the monocyte infiltration to the SOI and the production of IFN-γ upon antigen recall was markedly higher for DDA and DC-Chol based liposomes which exhibited a longer retention profile at the SOI. A long-term retention and slow release of liposome and vaccine antigen from the injection site hence appears to favor a stronger Th1 immune response.
Cell retention culture with an internal filter module: Continuous ethanol fermentation (1993)

A new internal filter feedback system with a stainless steel filter was introduced and its application for continuous ethanol fermentation was investigated. The filter performance was highly influenced by agitation speed and yeast concentration. Retention coefficient with a filter of 2 mum pore size was found more than 97.5%, and the filter was suitable for yeast separation. Maximum yeast concentration was 157 g/L and the best operable cell concentration was between 90 and 150 g/L. Which was similar to that obtained in the external membrane cell recycle culture. The cell concentration in the fermentor was maintained by manipulation of dilution rate and bleed ratio with the growth rate. The internal filter feedback system was successfully operated for more than 10 days. This study shows that the internal filter feedback system with a stainless steel filter can be used high-density cell culture and ethanol fermentation. Furthermore, it can be scaled up more easily than the external cell recycle system.
Endoscopic Spine Surgery and Instrumentation (2005)

Historical Background of Minimally Invasive Spine Surgery Endoscopic Surgical Equipment Anesthetic Considerations and Operating Room Set-Up for Minimally Invasive Endoscopic Spine Surgery Endoscopic-Assisted Transoral Odontoidectomy Anterior Endoscopic Cervical Microdiscectomy Percutaneous Endoscopic Cervical Discectomy and Stabilization Posterior Cervical Microendoscopic Discectomy (MED) and Laminoforaminotomy Microscopic Cervical Laminoplasty & Laminectomy Posterior Endoscopic Cervical Instrumentation Thoracoscopic Discectomy Posterolateral Endoscopic Thoracic Discectomy Endoscopic Transpedicular Thoracic Discectomy Thoracoscopic Management of Spinal Tumors Minimally Invasive Instrumentation, Correction and Fusion of Primary Thoracic and Thoracolumbar Scoliosis Thoracoscopic Approach for a Deformity with Frontier Instrumentation Combined Prone Position Thoracoscopic Anterior Release and Posterior Instrumentation for Deformity Surgery Thoracoscopic Decompression and Fixation (MACS-TL) Posterolateral Selective Endoscopic Discectomy. The YESS Technique Endoscopic Lumbar Foraminoplasty Lumbar Microendoscopic Laminoforaminotomy and Discectomy Paraspinal Edoscopic Laminectomy and Discectomy Endoscopic Pedicle Screw Instrumentation & Decompression (Endius) Endoscopic/Percutaneous Lumbar Pedicle Screw fixation Minimally Invasive Transforaminal Lumbar Interbody Fusion Percutaneous Translaminar Facet Screw Fixation Laparoscopic Fusion of the Lumbosacral Spine Endoscopic Lateral Transpsoas Lumbar Spine Fusion Gasless Endoscopic ALIF: The BERG Approach Percutaneous Vertebroplasty For Painful Vertebral Body Compression Fractures Kyphoplasty Intradiscal Electrothermal Therap Nucleoplasty Fluoroscopic Image Guided Spine Surgery Image-Guided Endoscopic and Minimally Invasive Spine Surgery Robotic Endoscopic Spine Surgery
Editor's Note: Hemolytic E. coli Promotes Colonic Tumorigenesis in Females (2019)

The editors are publishing this note to alert readers to concerns about this article (1). In Figs. 5, 6, and 7, tissue images were used to provide evidence for the presence of tumors. Examination by the editors and original reviewers determined that the images are either of insufficient resolution to detect the presence of tumors, or that there are no tumors present. Because satisfactorily corrected figures could not be provided, the editors are publishing this note to alert readers to these concerns.
Opening up attendance at HotNets (2018)

HotNets has historically been invitation-only. The SIGCOMM community has recently encouraged HotNets to allow broader participation. This note reports on a HotNets 2015 experiment with a more open attendance policy, and on the results of a post-workshop survey of the attendees. Based on this experiment and the survey, the HotNets Steering Committee believes it is possible for the workshop to support broader attendance, while preserving an atmosphere that encourages free-flowing discussions.
Reply to Elliott et al (1990)

No AccessJournal of Speech, Language, and Hearing ResearchLetter to the Editor1 Sep 1990Reply to Elliott et al Paula Tallal, andRutgers University Paula Tallal Rutgers University Google Scholar More articles by this author andRutgers University https://doi.org/10.1044/jshr.3303.619a SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationTrack Citations ShareFacebookTwitterLinked In REFERENCES Stark, R. E., & Tallal, P. (1988). Speech, language and reading disorders in children: Neuropsychological studies. Boston: Little, Brown and Company. Google Scholar Additional Resources FiguresReferencesRelatedDetails Volume 33Issue 3September 1990Pages: 619-619 Get Permissions Add to your Mendeley library HistoryReceived: Feb 20, 1990Accepted: Mar 2, 1990 Published in issue: Sep 1, 1990 Metrics Topicsasha-topicsasha-article-typesCopyright & PermissionsCopyright © 1990 American Speech-Language-Hearing AssociationPDF downloadLoading ...
COBALT(III) COMPLEXES OF THE TETRADENTATE MACROCYCLE 2, 12-DIMETHYL-3, 7, 11, 17-TETRAAZABICYCLO(11.3.1)HEPTADECA-1(17), 2, 11, 13, 15-PENTAENE (1974)

Abstract A series of cobalt(III) complexes of 2,12-dimethyl-3,7,11,17-tetraazabicyclo(11.3.1)heptadeca-1 (17),2,11,13,15-pentaene, CR, has been prepared. In addition to materials of common compositions and structures, the series includes a diiodo complex, a complex containing a bidentate nitrate ligand, an NO− derivative, and one in which ethylenediamine acts as a monodentate ligand with a dangling, unprotonated primary amine group.
Abstract 464: Hypoxia-induced miR-210 potentiates MDSC function through regulation of Arg1, Il-16 and Cxcl12, and promotes tumor growth (2015)

Abstract Myeloid Derived Suppressor Cells (MDSCs) are an integral component of the hypoxic tumor microenvironment. We demonstrated that hypoxia selectively induced miR-210 via HIF-1α in splenic MDSCs from tumor-bearing mice. Overexpression of miR-210 enhanced MDSC-mediated T cell suppression under normoxia, while targeting of hypoxic miR-210 decreased MDSC function. Our data show that miR-210 modulated MDSCs function by increasing arginase activity and NO production, although it had no effect on ROS or cytokine (IL-6 and IL-10) production and PD-L1 expression. A comprehensive approach using microRNA target predictions tool, transcriptome analysis, and luciferase reporter assay strongly pointed to the regulation of Arg1, Cxcl12 and Il16 at both mRNA and protein levels by miR-210 in splenic MDSCs. In mediating the immunosuppressive effects of miR-210, targeting of Arg1, and blockade of IL-16 and CXCL12, in splenic MDSCs from tumor-bearing mice under normoxia dramatically decreased MDSC-mediated T cell proliferative response and IFN-gamma production. Interestingly, miR-210 overexpressing MDSCs enhanced in vivo their immune suppressive activity resulting in an increase in 4T1 tumor growth. Therefore, the potential therapeutic use of miR-210 inhibitor oligonucleotide alone, or along with HIF-1α inhibitors as adjuvant tool combined to immunotherapeutic approaches, may be beneficial for boosting the immune system in cancer patients. Citation Format: Muhammad Zaeem Noman, Salem Chouaib. Hypoxia-induced miR-210 potentiates MDSC function through regulation of Arg1, Il-16 and Cxcl12, and promotes tumor growth. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 464. doi:10.1158/1538-7445.AM2015-464
Novel platforms for vascular carriers with controlled geometry (2011)

The first-generation platforms for vascular drug delivery adopted spherical morphologies. These carriers relied primarily on the size dependence of the enhanced permeability and retention effect to passively target vasculature, resulting in inefficient delivery due to significant variation in endothelial permeability. Enhanced delivery typically requires active targeting via receptor-mediated endocytosis by surface conjugation of targeting ligands. However, vascular carriers (VCs) still face numerous challenges en route to reaching their targets before delivery. The control of carrier shape offers opportunities to overcome in vivo barriers and enhance vascular drug delivery. Geometric features influence the ability of carrier particles to navigate physiological flow patterns, evade biological clearance mechanisms, sustain circulation, adhere to the vascular surface, and finally transport across or internalize into the endothelium. Although previous formulation strategies limited the fabrication of nonspherical carriers, numerous recent advances in both top-down and bottom-up fabrication techniques have enabled shape modulation as a key design element. As part of a series on vascular drug delivery, this review focuses on recent developments in novel vascular platforms with controlled geometry that enhance or modulate delivery functions. Starting with an overview of controlled geometry platforms, we review their shape-dependent functional characteristics for each stage of their vascular journey in vivo. We sequentially explore carrier geometries that evade reticuloendothelial system uptake, display enhanced circulation persistence and margination dynamics in flow, encourage adhesion to the vascular surface or extravasation through endothelium, and impact extravascular transport and cell internalization. The eventual biodistribution of VCs results from the culmination of their successive navigation of all these barriers and is profoundly influenced by their morphology. To enhance delivery efficacy, carrier designs synergistically combining controlled geometry with standard drug delivery strategies such as targeting moieties, surface decorations, and bulk material properties are discussed. Finally, we speculate on possibilities for innovation, harnessing shape as a design parameter for the next generation of vascular drug delivery platforms.