PD09-02 IMPLANTATION OF URINE-DERIVED STEM CELL FOR CHRONIC KIDNEY DISEASE INDUCED BY NEPHROTOXIC DRUG IN A RAT MODEL WITH RENAL ISCHEMIA (2019)

You have accessJournal of UrologyStem Cell Research (PD09)1 Apr 2019PD09-02 IMPLANTATION OF URINE-DERIVED STEM CELL FOR CHRONIC KIDNEY DISEASE INDUCED BY NEPHROTOXIC DRUG IN A RAT MODEL WITH RENAL ISCHEMIA Chao Zhang, Sunil K George, Mehran Abolbashar, Tae-Hyoung Kim, In Kap Ko, Yuanyuan Zhang*, John Jackson, Sang Jin Lee, James J Yoo, and Anthony Atala Chao ZhangChao Zhang More articles by this author , Sunil K GeorgeSunil K George More articles by this author , Mehran AbolbasharMehran Abolbashar More articles by this author , Tae-Hyoung KimTae-Hyoung Kim More articles by this author , In Kap KoIn Kap Ko More articles by this author , Yuanyuan Zhang*Yuanyuan Zhang* More articles by this author , John JacksonJohn Jackson More articles by this author , Sang Jin LeeSang Jin Lee More articles by this author , James J YooJames J Yoo More articles by this author , and Anthony AtalaAnthony Atala More articles by this author View All Author Informationhttps://doi.org/10.1097/01.JU.0000555260.97896.b8AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVES: Drug-induced nephrotoxicity often occurs in the patients with existing renal dysfunction or renal ischemia, potentially leading to chronic kidney disease (CKD). Prompt treatment of drug induced adverse effects is critical to prevent progression to end-stage renal disease. The goal of this study was to demonstrate the therapeutic potential of urine-derived stem cells (USC) to treat chronic kidney disease-induced by nephrotoxic drug and renal ischemia. METHODS: Human USC were collected, expanded and identified by flow cytometry analysis. The CKD model was induced by creating an ischemia-reperfusion injury followed by gentamicin injection. Twenty-eight immune deficient rats were divided into three groups: phosphate-buffered saline (PBS)-treated group (n=9), USC-treated group (n=9), and sham group with age-matched controls (n=10). Cell suspension of USC or PBS was bilaterally injected into the renal parenchyma (50x10^6/100µl/kidney) 9 weeks after the CKD model creation. Blood and urine samples were collected to test renal function (i.e, serum creatinine levels and glomerulus filtration rate). The kidneys were harvested 12 weeks after cell injection. Histologically, the number of affected glomeruli and tubular atrophy, levels of collagen deposition, interstitial fibrosis and inflammatory monocyte infiltration were examined. RESULTS: The USC used in this study expressed CD24+, CD29+, CD44+, CD90+, CD105+, CD146+, and SSEA4+ but did not express CD31, CD34, CD45 and STRO-1. Renal function in the animals treated with USC was significantly improved, as compared to those in the PBS-treated animals (p<0.05). The number of glomerular sclerosis and atrophic renal tubulars, amount of fibrotic area and monocyte infiltration significantly decreased in the USC-treated group as compared to the PBS group (p<0.05). CONCLUSIONS: The present study demonstrates the nephron-protective effect of USC on renal function via anti-inflammatory and anti-fibrotic activity in a rat model, which provides an alternative treatment option for CKD. Source of Funding: none Winston Salem, NC© 2019 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 201Issue Supplement 4April 2019Page: e154-e154 Advertisement Copyright & Permissions© 2019 by American Urological Association Education and Research, Inc.MetricsAuthor Information Chao Zhang More articles by this author Sunil K George More articles by this author Mehran Abolbashar More articles by this author Tae-Hyoung Kim More articles by this author In Kap Ko More articles by this author Yuanyuan Zhang* More articles by this author John Jackson More articles by this author Sang Jin Lee More articles by this author James J Yoo More articles by this author Anthony Atala More articles by this author Expand All Advertisement PDF downloadLoading ...