gebhart/patrepeval huggingface dataset:
Simultaneous measurement of benzo[a]pyrene‐inducedPig‐aandlacZmutations, micronuclei and dna adducts in mutaTMmouse (2011)

In this study we compared the response of the Pig-a gene mutation assay to that of the lacZ transgenic rodent mutation assay, and demonstrated that multiple endpoints can be measured in a 28-day repeat dose study. Muta™Mouse were dosed daily for 28 days with benzo[a]pyrene (BaP; 0, 25, 50 and 75 mg/kg body weight/day) by oral gavage. Micronucleus (MN) frequency was determined in reticulocytes (RETs) 48 hr following the last dose. 72 h following the last dose, mice were euthanized, and tissues (glandular stomach, small intestine, bone marrow and liver) were collected for lacZ mutation and DNA adduct analysis, and blood was evaluated for Pig-a mutants. BaP-derived DNA adducts were detected in all tissues examined and significant dose-dependent increases in mutant Pig-a phenotypes (i.e., RET(CD24-) and RBC (CD24-)) and lacZ mutants were observed. We estimate that mutagenic efficiency (i.e., rate of conversion of adducts into mutations) was much lower for Pig-a compared to lacZ, and speculate that this difference is likely explained by differences in repair capacity between the gene targets, and differences in the cell populations sampled for Pig-a versus lacZ. The BaP doubling doses for both gene targets, however, were comparable, suggesting that similar mechanisms are involved in the accumulation of gene mutations. Significant dose-related increases in % MN were also observed; however, the doubling dose was considerably higher for this endpoint. The similarity in dose response kinetics of Pig-a and lacZ provides further evidence for the mutational origin of glycosylphosphatidylinositol (GPI)-anchor deficiencies detected in the Pig-a assay.
Additive-mixing fuel-injection system for internal combustion engines (2023)

A system and method for fast control of the timing of combustion in an internal combustion engine, comprising actuating a fast-acting fuel-additive supply valve to meter a variable amount of fuel additive into a fuel stream, thereby forming an additive-enhanced fuel with an additive concentration that can be dynamically adjusted as fast as each engine cycle; injecting the additive-enhanced fuel directly or indirectly into a combustion chamber or into an intake port; and combusting the additive-enhanced fuel.
Iron Nanoparticle Size Control Through Reversible Magnetic Agglomeration. (2020)

Abstract not provided.
Synthesis of Enantiopure 8-Aminomethylindolizines from Glutamine by Stereoelectronically Controlled Cationic Cyclization (2000)

Starting from natural glutamine the synthesis of the 8aminomethylindolizine (4b) was accomplished.The construction of the ring system was performed by employing a cationic 6-exoπ-cyclization of an intermediate aziridinium salt.Transformation of the N,N-dibenzyl protected amine (4b) into the pharmacologically relevant target compound ( 11) is also describcd.The exploitation of cationic π-cyclizations in the construction of a variety of ring systems has been the object of intense studies in the field of heterocyclic chemistry. 1Employing N-pyrrole-terminated cyclization precursors this strategy gives access to tetrahydroindolizines and tetrahydropyrrolo[1 ,2-a]azepines. 2,3 As a part of our ongoing efforts to design selective dopamine D2/D3 autoreceptor agonists 4 which are of potential interest for the treatment of schizophrenia and cocaine craving, 5 we used cationic π-cyclizations for the construction of enantiomerically pure 6-and 7-dipropylaminotetrahydroindolizines revealing highly interesting receptor binding profiles. 6The ex-chiral pool synthesis of the 7-amino regioisomers was performed by chemoselective functionalization of asparagine (Asn), incorporation of a pyrrole moiety by Paal-Knorr reaction to furnish la and O-activation of the side chain. 7Since we were aware that sulfonylation of N,N-dibenzyl protected 1 ,2-amino alcohols and subsequent intermolecular nucleophilic substitution can result in rearrangements through aziridinium intermediates, 8 we elucidated the structure of the product very carefully.In fact, a 6-endo process leading to the indolizine (3a) was observed exclusively.5-Exo attack of 2a to give the pyrrolizine derivative (4a) could not bc detected.As an extension of our studies, we intended to investigate the synthesis of the homologous 1 ,2-amino alcohol (1b) which should bc available from natural glutamine (Gln).After O-activation the cyclization behavior of the resulting aziridinium derivative (2b) should be examined.Besides the application of the synthesis for extending our structure activity relationship
Purification of pseudouridylate synthetase I from Salmonella typhimurium (1978)

Pseudouridylate synthetase from Salmonella typhimurium has been purified 1,000 fold and is about 90% pure. The enzyme has a molecular weight of 50,000 daltons. In the presence of tRNA there is a change in molecular weight from 50.000 to 100.000. This change does not seem to be due to the formation of a tRNA-enzyme complex but rather to a tRNA induced dimerization. Other properties of the enzyme are described.
Performance of CDMA mobile communication systems using antenna arrays (1993)

The use antenna arrays in cellular code division multiple access mobile communications is studied. Techniques to localize each mobile within the cell and to generate tracking adaptive receive (and transmit) beams are addressed. The authors show that such enhancement of the CDMA system results in increased system capacity and improved robustness to power control errors. Preliminary simulation results are presented for capacity and robustness improvements for typical scenarios.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Profiling of Somatic Mutations in Phaeochromocytoma and Paraganglioma by Targeted Next Generation Sequencing Analysis (2015)

At least 12 genes (FH, HIF2A, MAX, NF1, RET, SDHA, SDHB, SDHC, SDHD, SDHAF2, TMEM127, and VHL) have been implicated in inherited predisposition to phaeochromocytoma (PCC), paraganglioma (PGL), or head and neck paraganglioma (HNPGL) and a germline mutation may be detected in more than 30% of cases. Knowledge of somatic mutations contributing to PCC/PGL/HNPGL pathogenesis has received less attention though mutations in HRAS, HIF2A, NF1, RET, and VHL have been reported. To further elucidate the role of somatic mutation in PCC/PGL/HNPGL tumourigenesis, we employed a next generation sequencing strategy to analyse "mutation hotspots" in 50 human cancer genes. Mutations were identified for HRAS (c.37G>C; p.G13R and c.182A>G; p.Q61R) in 7.1% (6/85); for BRAF (c.1799T>A; p.V600E) in 1.2% (1/85) of tumours; and for TP53 (c.1010G>A; p.R337H) in 2.35% (2/85) of cases. Twenty-one tumours harboured mutations in inherited PCC/PGL/HNPGL genes and no HRAS, BRAF, or TP53 mutations occurred in this group. Combining our data with previous reports of HRAS mutations in PCC/PGL we find that the mean frequency of HRAS/BRAF mutations in sporadic PCC/PGL is 8.9% (24/269) and in PCC/PGL with an inherited gene mutation 0% (0/148) suggesting that HRAS/BRAF mutations and inherited PCC/PGL genes mutations might be mutually exclusive. We report the first evidence for BRAF mutations in the pathogenesis of PCC/PGL/HNPGL.
Optimization of fullerene-based negative tone chemically amplified fullerene resist for extreme ultraviolet lithography (2014)

While the technological progress of Next Generation Lithography (NGL) steadily continues, further progress is required before successful insertion in high volume manufacturing is possible. A key issue is the development of new resists suitable to achieve higher lithographic resolution with acceptable sensitivity and line edge roughness. Molecular resists have been a primary focus of interest for NGL because they promise high resolution and small line edge roughness (LER), but no suitable resist candidate has emerged yet that fulfills all of the industry’s criteria. We have previously shown first extreme ultraviolet lithography (EUVL) exposures for a new fullerene derivative based three-component negative tone chemically amplified resist with suitable properties close to or within the target range of the resist metrics as set out in the International Technology Roadmap for Semiconductors for 2016. Here we present the results of our efforts to optimize the EUVL performance of our resist system especially with regards to LER.
Cancer of the cervix uteri (2018)

Abstract Since the publication of the last FIGO Cancer Report there have been giant strides in the global effort to reduce the burden of cervical cancer, with WHO announcing a call for elimination. In over 80 countries, including LMIC s, HPV vaccination is now included in the national program. Screening has also seen major advances with implementation of HPV testing on a larger scale. However, these interventions will take a few years to show their impact. Meanwhile, over half a million new cases are added each year. Recent developments in imaging and increased use of minimally invasive surgery have changed the paradigm for management of these cases. The FIGO Gynecologic Oncology Committee has revised the staging system based on these advances. This chapter discusses the management of cervical cancer based on the stage of disease, including attention to palliation and quality of life issues.
Supplementary Table S3 from Genomic Instability and Protumoral Inflammation Are Associated with Primary Resistance to Anti–PD-1 + Antiangiogenesis in Malignant Pleural Mesothelioma (2023)

&lt;p&gt;Adverse events emergent during treatment and related to experimental drugs by the investigators according to CTCAE Version 4.03.&lt;/p&gt;
Clinical and Biological Efficacy of Recombinant Human Interleukin-21 in Patients with Stage IV Malignant Melanoma without Prior Treatment: A Phase IIa Trial (2009)

rIL-21 administered at 30 microg/kg/d in 5-day cycles every second week is biologically active and well tolerated in patients with metastatic melanoma. Confirmed responses, including one CR, were observed.
Model-independent search for CP violation in D0→K−K+π−π+ and D0→π−π+π+π− decays (2013)

A search for CP violation in the phase-space structures of D 0 and D 0 decays to the final states
High-performance conformal sensors employing single-crystal silicon nanomembranes (2014)

We demonstrate light-weight, conformal, and high-performance flexible sensors fabricated on a large area (&gt;2 cm &times; 2 cm) silicon nanomembrane transferred onto a flexible substrate. Linear L13 photonic crystal microcavities are designed to provide high quality factors on the flexible platform. Subwavelength grating (SWG) couplers are employed in order to enable efficient light coupling to the device using a single mode fiber. Photonic crystal tapers are implemented at the strip-photonic crystal waveguide interfaces to minimize loss. Preliminary chemical sensing data suggests a sensitivity of 75nm/RIU. Bending tests are further performed in order to demonstrate sensitivityindependent operation.
Simultaneous detection and epitope mapping of anti-factor VIII antibodies (2008)

The development of antibodies (Abs) against infused factor VIII (FVIII) is currently one of the most serious complications in the treatment of patients suffering from haemophilia A. Improved prevention and eradication of these anti-FVIII Abs remain a challenge for both clinicians and scientists. Here we describe an immunoassay to simultaneously detect and map the epitope specificity of haemophilia A patients' inhibitors by screening plasma against both heavy and light chains (HC and LC) of human plasma-derived FVIII (pFVIII). The format used was a two-site sandwich assay, where one monoclonal antibody (mAb) specific for the HC or LC was first immobilized on beads, and then incubated with the different forms of pFVIII. After incubation with patients' plasma samples, binding was revealed by a phycoerythrin-labeled secondary Ab. Samples from haemophilia patients with autoantibodies (autoAb) or alloantibodies (alloAb) were screened in this format. The former preferentially recognized the LC, whereas the latter were directed against both LC and HC. This technology appears attractive as it is fast and requires only 100 microl of patient's plasma. Furthermore, not only are anti-FVIII Abs detected, but information on their epitopic specificity is also obtained.
Understanding and Improving Drilled-Down Information Extraction from Online Data Visualizations for Screen-Reader Users (2023)

Inaccessible online data visualizations can significantly disenfranchise screen-reader users from accessing critical online information. Current accessibility measures, such as adding alternative text to visualizations, only provide a high-level overview of data, limiting screen-reader users from exploring data visualizations in depth. In this work, we build on prior research to develop taxonomies of information sought by screen-reader users to interact with online data visualizations granularly through role-based and longitudinal studies with screen-reader users. Utilizing these taxonomies, we extended the functionality of VoxLens—an open-source multi-modal system that improves the accessibility of data visualizations—by supporting drilled-down information extraction. We assessed the performance of our VoxLens enhancements through task-based user studies with 10 screen-reader and 10 non-screen-reader users. Our enhancements “closed the gap” between the two groups by enabling screen-reader users to extract information with approximately the same accuracy as non-screen-reader users, reducing interaction time by 22% in the process.
Atomic resolution structure of pseudoazurin from the methylotrophic denitrifying bacteriumHyphomicrobium denitrificans: structural insights into its spectroscopic properties (2008)

The crystal structure of native pseudoazurin (HdPAz) from the methylotrophic denitrifying bacterium Hyphomicrobium denitrificans has been determined at a resolution of 1.18 A. After refinement with SHELX employing anisotropic displacement parameters and riding H atoms, R(work) and R(free) were 0.135 and 0.169, respectively. Visualization of the anisotropic displacement parameters as thermal ellipsoids provided insight into the atomic motion within the perturbed type 1 Cu site. The asymmetric unit includes three HdPAz molecules which are tightly packed by head-to-head cupredoxin dimer formation. The shape of the Cu-atom ellipsoid implies significant vibrational motion diagonal to the equatorial xy plane defined by the three ligands (two His and one Cys). The geometric parameters of the type 1 Cu site in the HdPAz structure differ unambiguously from those of other pseudoazurins. It is demonstrated that their structural aspects are consistent with the unique visible absorption spectrum.
Practical guide to skin prick tests in allergy to aeroallergens (2011)

This pocket guide is the result of a consensus reached between members of the Global Allergy and Asthma European Network (GA(2) LEN) and Allergic Rhinitis and its Impact on Asthma (ARIA). The aim of the current pocket guide is to offer a comprehensive set of recommendations on the use of skin prick tests in allergic rhinitis-conjunctivitis and asthma in daily practice. This pocket guide is meant to give simple answers to the most frequent questions raised by practitioners in Europe, including 'practicing allergists', general practitioners and any other physicians with special interest in the management of allergic diseases. It is not a long or detailed scientific review of the topic. However, the recommendations in this pocket guide were compiled following an in-depth review of existing guidelines and publications, including the 1993 European Academy of Allergy and Clinical Immunology position paper, the 2001 ARIA document and the ARIA update 2008 (prepared in collaboration with GA(2) LEN). The recommendations cover skin test methodology and interpretation, allergen extracts to be used, as well as indications in a variety of settings including paediatrics and developing countries.
Li+ Transport in Lithium Sulfonylimide−Oligo(ethylene oxide) Ionic Liquids and Oligo(ethylene oxide) Doped with LiTFSI (2006)

The Li+ environment and transport in an ionic liquid (IL) comprised of Li+ and an anion of bis(trifluoromethanesulfonyl)imide anion (TFSI-) tethered to oligoethylene oxide (EO) (EO(12)TFSI-/Li+) were determined and compared to those in a binary solution of the oligoethylene oxide with LiTFSI salt (EO(12)/LiTFSI) by using molecular dynamics (MD) simulations and AC conductivity measurements. The latter revealed that the AC conductivity is 1 to 2 orders of magnitude less in the IL compared to the oligoether/salt binary electrolyte with greater differences being observed at lower temperatures. The conductivity of these electrolytes was accurately predicted by MD simulations, which were used in conjunction with a microscopic model to determine mechanisms of Li+ transport. It was discerned that structure-diffusion of the Li+ cation in the binary electrolyte (EO(12)/LiTFSI-) was similar to that in EO(12)TFSI-/Li+ IL at high temperature (>363 K), thus, one can estimate conductivity of IL at this temperature range if one knows the structure-diffusion of Li+ in the binary electrolyte. However, the rate of structure-diffusion of Li+ in IL was found to slow more dramatically with decreasing temperature than in the binary electrolyte. Lithium motion together with EO(12) solvent accounted for 90% of Li+ transport in EO(12)/LiTFSI-, while the Li+ motion together with the EO(12)TFSI- anion contributed approximately half to the total Li+ transport but did not contribute to the charge transport in IL.
57.1: Invited Paper: Comparison of a‐Si and Poly‐Si for AMOLEDs (2004)

Abstract The characteristics including intrinsic property of a‐Si ‐TFT and LTPS TFT for the application used as OLED backplane are reviewed. Though a‐Si AMOLED shows better uniformity and is capable of driving OLED, the stability of a‐Si TFT under operation is still not acceptable, while LTPS TFT reveals satisfactory stability in AMOLED application. The stability of TFT under long‐term operation is remained to be the key issue for commercialization of a‐Si TFT AMOLED.
Human hippocampal pre-activation predicts behavior (2017)

The response to an upcoming salient event is accelerated when the event is expected given the preceding events - i.e. a temporal context effect. For example, naming a picture following a strongly constraining temporal context is faster than naming a picture after a weakly constraining temporal context. We used sentences as naturalistic stimuli to manipulate expectations on upcoming pictures without prior training. Here, using intracranial recordings from the human hippocampus we found more power in the high-frequency band prior to high-expected pictures than weakly expected ones. We applied pattern similarity analysis on the temporal pattern of hippocampal high-frequency band activity in single hippocampal contacts. We found that greater similarity in the pattern of hippocampal field potentials between pre-picture interval and expected picture interval in the high-frequency band predicted picture-naming latencies. Additional pattern similarity analysis indicated that the hippocampal representations follow a semantic map. The results suggest that hippocampal pre-activation of expected stimuli is a facilitating mechanism underlying the powerful contextual behavioral effect.
Implementing a Human-Aware Robot System (2006)

The presence of humans in the robot environment brings new challenges to the robotic research. From low level functions to high level planners, clearly the human has to be taken into account in all the layers of the robot control system. Indeed, the robot has to behave socially in order to interact friendly with its human partners. This paper describes the development of several components (human detection and tracking, planning and supervision) that take into account humans explicitly with some preliminary results of their integration.
Abstract 1559: TLR7/8 immune-stimulating antibody conjugates elicit robust myeloid activation leading to enhanced effector function and anti-tumor immunity in pre-clinical models (2019)

Deficient anti-tumor immunity often results from an immunosuppressive tumor microenvironment (TME) that renders antigen presenting cells (APCs) unable to effectively stimulate T cells. Recent studies indicate that local delivery of immunostimulatory adjuvants can activate tumor resident APCs, driving uptake, processing and presentation of tumor neoantigens to T cells that mediate anti-tumor immunity. To overcome challenges associated with intratumoral delivery of such adjuvants, we developed a novel class of TLR immune-stimulating antibody conjugates (TAC) that comprise a TLR7/8 agonist conjugated to tumor-targeting monoclonal antibodies. In vitro co-cultures with human cancer cell lines and leukocytes revealed that TACs potently activate primary APCs, leading to increased co-stimulatory molecule expression (e.g. CD40, CD86) and secretion of pro-inflammatory cytokines (e.g. TNFα). The TACs also enhanced antibody-mediated effector functions such as ADCC and ADCP. Surprisingly, these constructs also induced dendritic cell (DC) differentiation from monocytes, as measured by changes in cellular morphology and DC surface markers (e.g. CD14 downregulation). CyTOF-based analysis of intracellular signaling in human leukocytes revealed a unique signaling signature of the conjugate compared to a mixture of its components, suggesting a novel biological mechanism by which the conjugate stimulates APCs. Finally, we demonstrated in vivo efficacy in syngeneic tumor models in which TAC treatment led to tumor clearance and development of immunologic memory. These results provide a strong rationale for this technology as a platform for cancer immunotherapy.Citation Format: Shelley E. Ackerman, Joseph C. Gonzalez, Josh D. Gregorio, Jason C. Paik, Felix J. Hartmann, Justin A. Kenkel, Arthur Lee, Angela Luo, Cecelia I. Pearson, Murray L. Nguyen, Benjamin Ackerman, Lauren Y. Sheu, Richard P. Laura, Steven J. Chapin, Brian S. Safina, Sean C. Bendall, David Dornan, Edgar G. Engleman, Michael N. Alonso. TLR7/8 immune-stimulating antibody conjugates elicit robust myeloid activation leading to enhanced effector function and anti-tumor immunity in pre-clinical models [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 1559.