gebhart/patrepeval huggingface dataset:
ERK and mTORC1 Inhibitors Enhance the Anti-Cancer Capacity of the Octpep-1 Venom-Derived Peptide in Melanoma BRAF(V600E) Mutations (2021)

Melanoma is the main cause of skin cancer deaths, with special emphasis in those cases carrying BRAF mutations that trigger the mitogen-activated protein kinases (MAPK) signaling and unrestrained cell proliferation in the absence of mitogens. Current therapies targeting MAPK are hindered by drug resistance and relapse that rely on metabolic rewiring and Akt activation. To identify new drug candidates against melanoma, we investigated the molecular mechanism of action of the <i>Octopus Kaurna</i>-derived peptide, Octpep-1, in human BRAF(V600E) melanoma cells using proteomics and RNAseq coupled with metabolic analysis. Fluorescence microscopy verified that Octpep-1 tagged with fluorescein enters MM96L and NFF cells and distributes preferentially in the perinuclear area of MM96L cells. Proteomics and RNAseq revealed that Octpep-1 targets PI3K/AKT/mTOR signaling in MM96L cells. In addition, Octpep-1 combined with rapamycin (mTORC1 inhibitor) or LY3214996 (ERK1/2 inhibitor) augmented the cytotoxicity against BRAF(V600E) melanoma cells in comparison with the inhibitors or Octpep-1 alone. Octpep-1-treated MM96L cells displayed reduced glycolysis and mitochondrial respiration when combined with LY3214996. Altogether these data support Octpep-1 as an optimal candidate in combination therapies for melanoma BRAF(V600E) mutations.
An Undergraduate Low-Level Computer Communications Laboratory Oriented towards Industry (2000)

This paper describes an educational methodology that we have implemented at the Universidad de Vigo which is based on a real competitive environment. It involves developing a communication subsystem, specified by a standard. Intercommunication between subsystems must take place successfully according to that standard. However, students are free to incorporate new features, enhance others, or optimize aspects which are roughly described.
Anomalous Neber reaction. General method for the preparation of 3-carboxy-1-naphthylamines (1975)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAnomalous Neber reaction. General method for the preparation of 3-carboxy-1-naphthylaminesMichael E. Garst, Daniel D. Cox, Richard W. Harper, and D. S. KempCite this: J. Org. Chem. 1975, 40, 8, 1169–1170Publication Date (Print):April 1, 1975Publication History Published online1 May 2002Published inissue 1 April 1975https://pubs.acs.org/doi/10.1021/jo00896a038https://doi.org/10.1021/jo00896a038research-articleACS PublicationsRequest reuse permissionsArticle Views156Altmetric-Citations8LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A Molecularly Defined, Grafted Olefin Metathesis Catalyst from Tris(neopentyl)‐nitridomolybdenum(VI) (1996)

1,2-Addition of the surface silanol groups on SiO2 to the nitridomolybdenum fragment of the title compound and subsequent α-elimination of neopentane forms the catalytically active surface species I. This is the first case in which an alkylated complex is converted into a heterogeneous catalyst in a well-defined way. The immobilized compound is significantly more active in ring-opening metathesis polymerization than the molecular precursor.
THE PROTECTIVE EFFECT OF KLOTHO PROTEIN AGAINST OXIDATIVE STRESS (2004)

Matsukawa, N; Kida, I; Ohta, J; Yang, J; Rakugi, R; Nabeshima, Y; Ogihara, T Author Information
Nacre‐Inspired Composite Electrolytes for Load‐Bearing Solid‐State Lithium‐Metal Batteries (2019)

Solid-state lithium-metal batteries with solid electrolytes are promising for next-generation energy-storage devices. However, it remains challenging to develop solid electrolytes that are both mechanically robust and strong against external mechanical load, due to the brittleness of ceramic electrolytes and the softness of polymer electrolytes. Herein, a nacre-inspired design of ceramic/polymer solid composite electrolytes with a "brick-and-mortar" microstructure is proposed. The nacre-like ceramic/polymer electrolyte (NCPE) simultaneously possesses a much higher fracture strain (1.1%) than pure ceramic electrolytes (0.13%) and a much larger ultimate flexural modulus (7.8 GPa) than pure polymer electrolytes (20 MPa). The electrochemical performance of NCPE is also much better than pure ceramic or polymer electrolytes, especially under mechanical load. A 5 × 5 cm<sup>2</sup> pouch cell with LAGP/poly(ether-acrylate) NCPE exhibits stable cycling with a capacity retention of 95.6% over 100 cycles at room temperature, even undergoes a large point load of 10 N. In contrast, cells based on pure ceramic and pure polymer electrolyte show poor cycle life. The NCPE provides a new design for solid composite electrolyte and opens up new possibilities for future solid-state lithium-metal batteries and structural energy storage.
A Programmable Bandpass Filter With Simultaneously Reconfigurable Working Frequency and Bandwidth (2019)

A sharp-rejection programmable bandpass filter (BPF) with transmission zeros (TZs) and simultaneous continuous-type reconfiguration in terms of center frequency and bandwidth is presented. With the user-predefined filtering responses, it promises to simplify modern RF system architectures and adapts to flexible front-end designs. The proposed controllable BPF is composed of two building blocks, namely the resonant-inverter (RI) and the shunt resonator. The BPF programmability relies on the adaptive change of the resonances in these two circuit parts. When the shunt resonances that define the overall TZs move out of the frequency span defined by the even-odd-mode-RI-subnetwork natural frequencies, the bandwidth increases; if all the resonances are shifted with their relative spacing maintained, the center frequency is varied for constant absolute bandwidth. A proof-of-concept reconfigurable BPF design example is implemented, showing a highly flexible bandwidth tuning ratio of 5:1 and center-frequency tuning ratio of 2:1.
Synthesis and Reactivity of Mevinolin-Like Lactone Precursors (1994)

Abstract The synthesis of mevinolin-like lactone precursors and the evaluation of their ability in the N-alkylation of N-potassium-phthalimide are reported.
Hierarchical Motion Vector Estimation With Decision Criterion On Buffer Memory Status (2005)

dbstract- This paper presents a new approach of the motion vector estimation, which saves the total number of data on motion vectors in coded images and produce good quality of reconstructed images. The estimation scheme is based on the hierarchical structure to accelerate the processing and adopts a decision criterion to control the amount of incoming data into transmission buffer memory. The decision factors are the prediction error of motion vector on low resolution images and the status (fullness) of transmission buffer memory. To demonstrate the efficience of the proposed process, the comparison of signal to noise ratio(PSNR) with and without using the proposed decision criterion is observed. SUMMARY Due to the limited transmission channel capacity, the motion vector estimation plays an important role to compress the transmitted sequential image data. The quality of reconstructed image is, therefore, strictly related to the motion vectors and its accuracy. However, besides of the accuracy of motion vector, the status of the transmission buffer memory is another important factor for the image quality. The amount of data representing image is directly controlled by buffer status. In case of that it is unnecessary to assign more bits to the motion vectors, it is encourage to assign the saved bits to image information instead. Therefore, the main focus of this paper is reducing the total amount of motion vector data to help assign more bits to image data considering the accuracy of the motion vector and buffer status. To reduce the number of motion vector data, a two-level resolution structure is proposed. The lower resolution image is generated from the original image by reducing it into half resolution in horizontal and vertical directions, therefore it is one quarter size of original image. Gaussian pyramid coding technique 111 is used to reduce the resolution because of its fast performance of removing the spatial redundancies of the images. Motion vectors on low resolution images, with the same size of block and search area in higher resolutions, are estimated. From this coarse motion vector estimation, total number of motion vectors are reduced by factor of four and actual estimated area expands four times larger than one in higher resolution. In other words, one motion vector in lower resolution representsfour motion vectors in higher resolution and the scale of motion vectors detected in low resolution represents twice larger scale in higher resolution. For the accuracy of motion vector, the Block Matching Algorithm (BMA) is used. In BMA, the motion vector is estimated by minimizing the prediction error. The Prediction error of vector (cw,B) is defined as follows ;
Adaptive Modulation for Underwater Acoustic OFDM Communication (2019)

Rapidly varying multipath propagation and limitation of bandwidth are major constraints of underwater acoustic (UA) communication systems. In order to improve the spectral efficiency, adaptive modulation schemes are considered in the context of UA orthogonal frequency division multiplexing (OFDM) systems. The objective of this paper is developing self-adapting modulation algorithms by analyzing the characteristics of the UA channel and varying transmission parameters according to the channel condition. The signal-to-noise ratio (SNR) is estimated at the receiver and used as a performance metric to switch the modulation mode and fed back to the transmitter for data transmission. Simulation results show that the system performance is better than fixed modulation schemes.
Phase 1 dose-finding study of rebastinib (DCC-2036) in patients with relapsed chronic myeloid leukemia and acute myeloid leukemia (2016)

A vailable tyrosine kinase inhibitors for chronic myeloid leukemia bind in an adenosine 5'-triphosphate-binding pocket and are affected by evolving mutations that confer resistance. Rebastinib was identified as a switch control inhibitor of BCR-ABL1 and FLT3 and may be active against resistant mutations. A Phase 1, first-in-human, single-agent study investigated rebastinib in relapsed or refractory chronic or acute myeloid leukemia. The primary objectives were to investigate the safety of rebastinib and establish the maximum tolerated dose and recommended Phase 2 dose. Fifty-seven patients received treatment with rebastinib. Sixteen patients were treated using powder-in-capsule preparations at doses from 57 mg to 1200 mg daily, and 41 received tablet preparations at doses of 100 mg to 400 mg daily. Dose-limiting toxicities were dysarthria, muscle weakness, and peripheral neuropathy. The maximum tolerated dose was 150 mg tablets administered twice daily. Rebastinib was rapidly absorbed. Bioavailability was 3- to 4-fold greater with formulated tablets compared to unformulated capsules. Eight complete hematologic responses were achieved in 40 evaluable chronic myeloid leukemia patients, 4 of which had a T315I mutation. None of the 5 patients with acute myeloid leukemia responded. Pharmacodynamic analysis showed inhibition of phosphorylation of substrates of BCR-ABL1 or FLT3 by rebastinib. Although clinical activity was observed, clinical benefit was insufficient to justify continued development in chronic or acute myeloid leukemia. Pharmacodynamic analyses suggest that other kinases inhibited by rebastinib, such as TIE2, may be more relevant targets for the clinical development of rebastinib (<i>clinicaltrials.gov Identifier:00827138</i>).
Oxylipins act as determinants of natural product biosynthesis and seed colonization in Aspergillus nidulans (2005)

Secreted, hormone-like lipogenic molecules, called oxylipins, mediate the balance of asexual to sexual spore ratio in Aspergillus nidulans. Oxylipin production in this fungus is dependent on developmental regulation of three conserved fatty acid oxygenases, PpoA, PpoB and PpoC. Here, we show that in addition to altering spore ratios, loss of ppo genes affect natural product biosynthesis and seed colonization. DeltappoA;DeltappoC and DeltappoA;DeltappoB;DeltappoC mutants were unable to produce the mycotoxin sterigmatocystin (ST) in vitro or in planta but in contrast overproduced the antibiotic penicillin (PN). These findings were correlated with decreased expression of genes involved in ST biosynthesis and increased expression of a PN biosynthetic gene, thus suggesting that oxylipin species regulate secondary metabolites at the transcriptional level. Additionally, the DeltappoA;DeltappoC and the DeltappoA;DeltappoB;DeltappoC mutants were defective in colonization of peanut seeds as reflected by a decrease in conidiation and production of the seed degradative enzyme lipase. These results indicate that oxylipin production is important for host colonization and mycotoxin production and may provide a promising target for future control strategies.
Eggert, Gustafsson, and Rommer Reply: (2001)

In a comment by A.A. Zvyagin the phase diagram in our Letter [Phys. Rev. Lett. 86, 516 (2001)] was critisized of being incomplete and a new fixed point was suggested. We show that this point is in fact not a fixed point and that the phase diagram is correct as presented.
Diagnosis of respiratory diseases using the proteomic analysis of exhaled breath condensate (2017)

Investigation of exhaled breath condensate (EBC) is a noninvasive diagnostic method in respiratory diseases. The objective of this study was to compare EBC protein spectrum in healthy volunteers and in patients with chronic obstructive pulmonary disease (COPD), pneumonia and lung cancer (NSCLC), as well as to assess a role of proteomic analysis of EBC for diagnosis and differential diagnosis of these diseases. Methods . We examined 18 patients with COPD, 13 patients with community-acquired pneumonia, 26 patients with lung cancer and 24 healthy non-smoking volunteers. EBC was collected using ECoScreen system (VIASYS Healthcare, Germany) and a standardized method. EBC-samples were lyophilized, hydrolyzed and analyzed by HPLC and tandem mass spectrometry. To identify proteins, we used Mascot (Matrix Science, UK) and IPI-human (version 3.82) databases provided by the European Bioinformatics Institute. Results . Proteomic analysis of EBC identified more than 300 different proteins; most of them were types I and II cytoskeletal keratins. Cytokeratin 5, 6, and 14 concentrations in EBC of NSCLC patients were significantly higher than that in healthy volunteers. Dermcidin, immunoglobulin alpha, kininogen, cytoplasmic actin, serum albumin, and Zn-alpha2-glycoprotein were identified in EBC of healthy volunteers and patients with COPD and pneumonia. High concentration of peroxiredoxin in EBC of COPD patients could be due to severe oxidative stress. High levels of acute-phase and hypoxia proteins (annexins A1 and A2, HSP90B, cystatins M and B, collagen and histones fragments) were detected in EBC of pneumonia patients. Also, β- и α-subunit of hemoglobin, nuclear ubiquitin casein (NUCKS), POTEE, high mobility group protein (HMG-I/HMG-Y) and lactoferrin were identified in EBC of NSCLC patients. Conclusion . We found that EBC in healthy nonsmokers and in patients with COPD, pneumonia and NSCLC had characteristic protein spectrum. Most of the identified proteins could be used for diagnosis of these diseases.
A nano-scale molecular rotor device for high density memory application (2009)

A novel electrically driven molecular rotor device has been designed and fabricated. NDR behaviors were observed owing to the rotation motion on the Cu axel. This is the first time that a rotation induced NDR effect on a solid support was observed. The rotation of the molecules was also confirmed using the optical absorption spectroscopy. From temperature dependence measurements, the activation energy of the rotation was estimated to be 0.3 eV based on the Arrhenius plot.
A transcription factor active on the epidermal growth factor receptor gene. (1988)

We have developed an in vitro transcription system for the epidermal growth factor receptor (EGFR) oncogene by using nuclear extracts of A431 human epidermoid carcinoma cells, which overproduce EGFR. We found that a nuclear factor, termed EGFR-specific transcription factor (ETF), specifically stimulated EGFR transcription by 5- to 10-fold. In this report, ETF, purified by using sequence-specific oligonucleotide affinity chromatography, is shown by renaturing material eluted from a NaDodSO4/polyacrylamide gel to be a protein with a molecular mass of 120 kDa. ETF binds to the promoter region, as measured by DNase I "footprinting" and gel-mobility-shift assays, and specifically stimulates the transcription of the EGFR gene in a reconstituted in vitro transcription system. These results suggest that ETF could play a role in the overexpression of the cellular oncogene EGFR.
Ureteral Pseudodiverticulum Associated with Absorbable Suture Clips after Laparoscopic Pyeloplasty: Case Report (2005)

A 48 year-old woman with pyelonephritis was found to have bilateral ureteropelvic junction (UPJ) obstruction and a nonfunctioning right kidney. She initially underwent a laparoscopic left nondismembered pyeloplasty using absorbable polydioxanone Lapra-Ty suture clips (Ethicon Endosurgery, Cincinnati, OH) to secure the anastomosis. An antegrade endopyelotomy was later necessitated. Both procedures were complicated by postoperative bacteruria and funguria. She then underwent a laparoscopic dismembered pyeloplasty, again utilizing Lapra-Ty suture clips to secure the anastomosis. Postoperatively, her course was complicated by anastomotic extravasation, bacteriuria, and funguria. Subsequently, an antegrade nephrostogram revealed a 2.5-cm diverticulum just distal to the UPJ, which contained numerous 2- to 4-mm filling defects. Nephroscopic exploration of the pseudodiverticulum revealed numerous Lapra-Ty clips, which were basket extracted. The pseudodiverticulum was fulgurated with a holmium laser. She eventually had restricturing with recurrence of the pseudodiverticulum and was treated successfully by open ureterocalicostomy.
Complete plastid genome sequence of Daucus carota: Implications for biotechnology and phylogeny of angiosperms (2006)

The carrot plastid genome contains a number of dispersed direct and inverted repeats scattered throughout coding and non-coding regions. This is the first sequenced plastid genome of the family Apiaceae and only the second published genome sequence of the species-rich euasterid II clade. Both MP and ML trees provide very strong support (100% bootstrap) for the sister relationship of Daucus with Panax in the euasterid II clade. These results provide the best taxon sampling of complete chloroplast genomes and the strongest support yet for the sister relationship of Caryophyllales to the asterids. The availability of the complete plastid genome sequence should facilitate improved transformation efficiency and foreign gene expression in carrot through utilization of endogenous flanking sequences and regulatory elements.
Genome-wide association study identifies a missense variant at APOA5 for coronary artery disease in Multi-Ethnic Cohorts from Southeast Asia (2017)

Recent genome-wide association studies (GWAS) have identified multiple loci associated with coronary artery disease (CAD) among predominantly Europeans. However, their relevance to multi-ethnic populations from Southeast Asia is largely unknown. We performed a meta-analysis of four GWAS comprising three Chinese studies and one Malay study (Total N = 2,169 CAD cases and 7,376 controls). Top hits (P < 5 × 10<sup>-8</sup>) were further evaluated in 291 CAD cases and 1,848 controls of Asian Indians. Using all datasets, we validated recently identified loci associated with CAD. The involvement of known canonical pathways in CAD was tested by Ingenuity Pathway Analysis. We identified a missense SNP (rs2075291, G > T, G185C) in APOA5 for CAD that reached robust genome-wide significance (Meta P = 7.09 × 10<sup>-10</sup>, OR = 1.636). Conditional probability analysis indicated that the association at rs2075291 was independent of previously reported index SNP rs964184 in APOA5. We further replicated 10 loci previously identified among predominantly Europeans (P: 1.33 × 10<sup>-7</sup>-0.047). Seven pathways (P: 1.10 × 10<sup>-5</sup>-0.019) were identified. We identified a missense SNP, rs2075291, in APOA5 associated with CAD at a genome-wide significance level and provided new insights into pathways contributing to the susceptibility to CAD in the multi-ethnic populations from Southeast Asia.
Thermal Stability of the Li(Ni[sub 0.8]Co[sub 0.15]Al[sub 0.05])O[sub 2] Cathode in the Presence of Cell Components (2006)

The cathode, a potential candidate for hybrid electric vehicle applications, has been electrochemically charged in powder form without a carbon additive and binder. The thermal stability of the resulting powder was studied by thermal gravimetric analysis (TGA), gas chromatography/mass spectrometry, and X-ray diffraction techniques under different gas flows. The transformation of -layered material to the -type structure material and/or to nickel metal was correlated to the oxidizing/reducing properties of the TGA gas flow under which the thermal decomposition of the occurred. Differential scanning calorimetry measurements were performed on powder in the presence of solvent, salt, or binder independently. The reactivity at between and ethylene carbonate (EC) solvent was found to be dependent on the oxide/EC weight ratio. The exothermic reaction observed in the presence of other solvents was not greatly affected, as long as the oxide/solvent weight ratios were kept very close to one another. The salt, when added to the charged oxide powder, was found to shift the exothermic reaction to when it was dissolved in the electrolyte and when it was added in the solid form. When polyvinylidene fluoride binder was added to powder, the exothermic reaction occurred at high temperatures . The initiation of the exothermic reaction has been primarily attributed to the oxidation of the electrolyte by the oxygen gas released from after the collapse of its layered structure.