gebhart/patrepeval huggingface dataset:
Time‐Lapse Microscopy Approaches to Track Cell Cycle and Lineage Progression at the Single‐Cell Level (2013)

Time-lapse microscopy can be described as the repeated collection of an image (in n-dimensions; x, y, z, λ) or field of view from a microscope at discrete time intervals. The duration of the time interval defines the temporal resolution, which in turn characterizes the type of event detected. This unit describes the implementation of time-lapse microscopy to link initial cell cycle position during acute exposures to anti-cancer agents with anti-proliferative consequences for individual cells. The approach incorporates fundamental concepts arising from the ability to capture simple video sequences of cells from which it is possible to extract kinetic descriptors that reflect the interplay of mitosis and cell death in the growth of an unsynchronized tumor population. Utilizing a multi-well format enables the user to screen different drug derivatives, multiple dose ranges, or cell cultures with unique genetic backgrounds. The objective of this unit is to present the basic methodology for capturing time-lapse sequences and touch upon subsequent mining of the data for deriving event curves and possible cell lineage maps.
Design of a Cavity Heat Pipe Receiver Experiment (1992)

&lt;div class="htmlview paragraph"&gt;A cavity heat pipe experiment has been designed to test the critical issues involved with incorporating thermal energy storage canisters into a heat pipe. The experiment is a replication of the operation of a heat receiver for a Brayton solar dynamic power cycle. The heat receiver is composed of a cylindrical receptor surface and an annular heat pipe with thermal energy storage canisters and gaseous working fluid heat exchanger tubes surrounding it. Hardware for the cavity heat pipe experiment will consist of a sector of the heat pipe, complete with gas tube and thermal energy storage canisters. Thermal cycling tests will be performed on the heat pipe sector to simulate the normal energy charge/discharge cycle of the receiver in a spacecraft application.&lt;/div&gt;
Watt-Level, Direct RF Modulation in CMOS SOI With Pulse-Encoded Transitions for Adjacent Channel Leakage Reduction (2019)

This article reports signal processing and circuit techniques for direct RF signal modulation. A pulse-encoded transition (PET) technique is introduced to reduce undesired harmonic distortion (HD) and adjacent channel leakage ratio (ACLR) generated by the direct RF modulation. To enable PET RF switching, two variations of a high-power, stacked-FET switch modulator, an 8and 12-device stack, are designed in 45-nm silicon on insulator (SOI) CMOS. The switch design uses a tapering design to significantly improve power handling with minimal impact to switching speed. The modulators have P1 dB values between 34 and 39 dBm while demonstrating a modulation bandwidth of nearly 500 MHz with a 1-GHz carrier. The input referred third order intercept point (IIP3) is between 46 and 61 dBm. Additionally, ACLR measurements of up to -50 dBc are demonstrated using the proposed PET technique at 30-dBm output power. To the best of our knowledge, this is record power handling and ACLR for a CMOS switch.
Environmental Candida auris and the Global Warming Emergence Hypothesis (2021)

Global warming was proposed to be a contributing cause for the nearly simultaneous emergence of different clades of <i>Candida auris</i> as a nosocomial pathogen in different continents. The global warming emergence hypothesis posits that <i>C. auris</i> existed in the environment prior to its clinical recognition and became pathogenic for humans because of thermal adaptation in response to climate change. The isolation of <i>C. auris</i> from two sites in the remote Andaman Islands establishes it as an environmental organism, a necessary condition for the hypothesis. The observation that one environmental isolate grew slower at mammalian temperatures than clinical strains is consistent with the notion that their ancestor recently adapted to higher temperatures. The knowledge that <i>C. auris</i> can be recovered from the environment should prompt additional searches to define its ecological niches, and the analysis of future environmental isolates will provide evidence for validating or refuting the global warming emergence hypothesis.
Establishment of chronic postsurgical pain model and analysis of its therapeutic target (2020)

Not published
EUV pattern defect detection sensitivity based on aerial image linewidth measurements (2009)

As the quality of EUV-wavelength mask inspection microscopes improves over time, the image properties and intensity profiles of reflected light can be evaluated in ever-greater detail. The SEMATECH Berkeley Actinic Inspection Tool (AIT) is one such microscope, featuring mask resolution values that match or exceed those available through lithographic printing in current photoresists. In order to evaluate the defect detection sensitivity of the AIT for dense line patterns on typical masks, the authors study the linewidth roughness (LWR) on two masks, as measured in the EUV images. They report the through-focus and pitch dependence of contrast, image log slope, linewidth, and LWR. The AIT currently reaches LWR 3σ values close to 9nm for 175nm half-pitch lines. This value is below 10% linewidth for nearly all lines routinely measured in the AIT. Evidence suggests that this lower level may arise from the mask’s inherent pattern roughness. While the sensitivity limit of the AIT has not yet been established, it is clear that the AIT has the required sensitivity to detect defects that cause 10% linewidth changes in line sizes of 125nm and larger.
Flexible Octopus‐Shaped Hydrogel Particles for Specific Cell Capture (2016)

Multiarm hydrogel microparticles with varying geometry are fabricated to specifically capture cells expressing epithelial cell adhesion molecule. Results show that particle shape influences cell-capture efficiency due to differences in surface area, hydrodynamic effects, and steric constraints. These findings can lead to improved particle design for cell separation and diagnostic applications.
Optical characterisation of photonic wire and photonic crystal waveguides fabricated using nanoimprint lithography (2006)

We have characterised photonic-crystal and photonic-wire waveguides fabricated by thermal nanoimprint lithography. The structures, with feature sizes down below 20 nm, are benchmarked against similar structures defined by direct electron beam lithography.
In Vitro Preactivation of PBMCs with PM-mb21-41BBL Particles Stimulates in Vivo Expansion of NK Cells Under Low IL-2 in NSG Mice (2014)

Abstract NK cell immunotherapy as a cancer treatment shows promise, but methods for consistent expansion of NK cells from a small fraction (~5%) of peripheral blood mononuclear cells (PBMCs) to therapeutically effective amounts are not widely accessible. Strategies that promote in vivo expansion rely on high-dose IL-2 in combination with lymphodepletion and suffer from severe treatment related toxicities as well as limited expansion. On the other hand, in vitro methods are available for robust NK cell expansion, but rely on prolonged in vitro co-culture with feeder cells that is costly and complex and requires high dose IL-2 (25,000-35,000 U) for persistence after injection into mice. An optimal NK cell expansion method would rely on off the shelf reagents, would not require long-term complex cultures, and would induce a rapid, sustained in vivo expansion of NK cells from unsorted PBMCs under low concentration of IL-2. We have developed a method to prepare particles from K562 cells engineered to express specific cytokines, and such particles stimulate NK cell expansion. Particles prepared using K562 cells expressing IL-21 and 41BBL (PM21 particles) cause highly specific expansion of cytotoxic NK cells from unsorted PBMCs that achieves 95% NK cells in ~14 days and ~10,000 fold expansion of NK in about 21 days. NK cell expansion is consistent using different preparations of PM-particles or leukocyte sources, and the PM-particles retain expansion efficacy with storage. The PM-mb21-41BBL particles were also used to stimulate in vivo NK cell expansion in NSG mice under ultralow IL-2 (1,000 units per injection, 3 injections per week, per mouse). Unsorted human PBMCs, either shortly pre-activated in culture for two days or not pre-activated, were injected (2 x 106 PBMCs per mice by i.p.) and human CD56+CD3- NK cells and other relevant hCD45+ lymphocytes were monitored in the peripheral blood over time and in tissues and fluids at the time of euthanization. In vivo NK cell expansion was observed in mice injected with PBMCs that were pre-activated with PM21 particles. The extent of NK cell expansion observed in the peripheral blood was in levels that would be relevant for clinical cancer treatment (&gt;50,000 NK cells/mL of mouse blood 14 days post injection). In vivo NK cell expansion was further confirmed with analogous experiments using PBMCs that were stained with CellTrace Violet to monitor proliferation. Notably, NK cells were also found in spleen, bone marrow, lung, liver and brain. These results taken together provide proof of principle that PM-particle technology is effective for direct in vivo NK cell expansion from unsorted PBMCs under low dose IL-2. Disclosures No relevant conflicts of interest to declare.
Analyse asymptotique de certains filtres de Wiener à rang réduit (2003)

National audience
Performance of shared and dedicated resources in WCDMA (2002)

For data transmission WCDMA offers dedicated transport channels (DCH) and common transport channels, i.e. random access channels (RACH) and forward access channels (FACH). Both types of transport channels have different characteristics in terms of transmission performance and radio resource usage. In this paper these characteristics are described and a simulation model for the performance evaluation is presented. The effect of some parameters influencing the performance and efficiency of the different transport channel types is discussed, e.g. channel configuration, traffic load and traffic characteristics. It is demonstrated that common transport channels provide better performance than dedicated channels for a low traffic load of bursty traffic. For low data volumes they are also more resource efficient. However for an increasing traffic load dedicated channels provide a better service quality and radio resource utilisation.
Properties of Phosphorescence Polymer Light Emitting Diodes with PVK: Ir(ppy)3:PFO:Ir(pq)2acac Emission Layer (2010)

Abstract Phosphorescence polymer LEDs (PhPLEDs) with the structure of ITO/PEDOT:PSS/PVK:Ir(ppy)3:PFO:Ir(pq)2acac/TPBI/LiF/Al were fabricated to investigate the effects of the doping concentration of Ir(pq)2acac on the electrical and optical properties of the devices. PVK(poly-vinylcarbazole) and Ir(ppy)3[tris(2-phenylpyridine)iridium(III)] polymers were used as the host and guest materials, respectively. PFO[poly(9,9-dioctylfluorene)] and Ir(pq)2(acac)[bis(2-phenyl-1-quinoline)iridiumacetyl acetonate] were also introduced as blue and red component guest materials for the white emission. The concentration ratio of PVK:Ir(ppy)3:PFO was fixed at 100:2.0:1.0 vol%. The Ir(pq)2acac concentration in the emission layer was varied from 1.0 to 5.0 vol%. White PhPLEDs were obtained for the samples with 5.0 vol% of Ir(pq)2acac, for which the luminance and current density were about 2530 cd/m2 and 720 mA/cm2 at 8 V, respectively. The CIE color coordinates were x, y = 0.33, 0.33, showing a pure white color. Keywords: CIE color coordinatesIr(ppy)3 Ir(pq)2acacluminancephosphorescencepolymer light emitting diodePVKwhite emission Acknowledgments This work was supported by a Korea Research Foundation Grant funded by the Korean Government (KRF-2008-521-D00261).
Interaction protocols as design abstractions for business processes (2005)

Business process modeling and enactment are notoriously complex, especially in open settings, where business partners are autonomous, requirements must be continually finessed, and exceptions frequently arise because of real-world or organizational problems. Traditional approaches, which attempt to capture processes as monolithic flows, have proven inadequate in addressing these challenges. We propose (business) protocols as components for developing business processes. A protocol is an abstract, modular, publishable specification of an interaction among different roles to be played by different participants. When instantiated with the participants' internal policies, protocols yield concrete business processes. Protocols are reusable and refinable, thus simplifying business process design. We show how protocols and their composition are theoretically founded in the phi;-calculus.
Effects of Long-term Irrigation with Water from Wastewater Receiving River on Soil Chemical and Microbiological Properties in Hebei, China (2015)

In arid and semi-arid regions in China, a large amount of treated wastewater is discharged into rivers that supply water for agriculture. Treated wastewater contains not only valuable nutrients, but also toxic contaminants that might affect soil quality. This study investigated how long-term irrigation with water from a wastewater receiving river affected soil chemical and microbiological properties in Hebei Province, China. Soil samples were collected from three river water irrigated fields adjacent to Wangyang River, and three groundwater irrigated fields far from the river. The results showed that irrigation with river water for more than 20 years had significantly increased dissolved organic C, microbial biomass C and permanganate oxidizable C contents, and -glucosidase, urease, alkaline phosphatase, arylsulphatase and dehydrogenase activities in the surface soil, reflecting a stimulated microbial activity in river water irrigated soils. Long-term irrigation with river water has also resulted in accumulation of Hg, Cd, As, Pb, Cu, Cr and Zn in the soil, and may pose potential risk to soil quality and food safety. We conclude that although the river water irrigation has beneficial effects on soil microbiological characteristics, a controlled irrigation management based on periodic monitoring of soil quality parameters is needed to ensure its safe and long-term use in agriculture.
Figure S4 from Genetic Alterations in the PI3K/AKT Pathway and Baseline AKT Activity Define AKT Inhibitor Sensitivity in Breast Cancer Patient-derived Xenografts (2023)

&lt;p&gt;Analysis of relevant signaling pathways, proliferation, apoptosis and S-phase entry in relation with AZD5363 resistance&lt;/p&gt;
Real-time modulation of perceptual eye dominance in humans (2014)

Ocular dominance (OD) has long served as the model for neural plasticity. The shift of OD has been demonstrated by monocular deprivation in animals only during early visual development. Here, for the first time, we show that perceptual eye dominance can be modulated in real time in normal human adults by varying the spatial image content of movies seen dichoptically by the two eyes over a period as short as 2.5 h. Unlike OD shifts seen in early visual development, this modulation in human eye dominance is not simply a consequence of reduced interocular correlation (e.g. synchronicity) or overall contrast energy, but due to the amplitude reductions of specific image components in one eye's view. The spatial properties driving this eye dominance change suggest that the underlying mechanism is binocular but not orientationally selective, therefore uniquely locating it to layer 4 B of area V1.
Effects of different doses in continuous veno‐venous haemofiltration on outcomes of acute renal failure: A prospective randomised trial (2002)

Mortality among these critically ill patients was high, but increase in the rate of ultrafiltration improved survival significantly We recommend that ultrafiltration should be prescribed according to patient's bodyweight and should reach at least 35 ml/h(-1)/kg(-1).
A Nonintegrative Lentiviral Vector-Based Vaccine Provides Long-Term Sterile Protection against Malaria (2012)

Trials testing the RTS,S candidate malaria vaccine and radiation-attenuated sporozoites (RAS) have shown that protective immunity against malaria can be induced and that an effective vaccine is not out of reach. However, longer-term protection and higher protection rates are required to eradicate malaria from the endemic regions. It implies that there is still a need to explore new vaccine strategies. Lentiviral vectors are very potent at inducing strong immunological memory. However their integrative status challenges their safety profile. Eliminating the integration step obviates the risk of insertional oncogenesis. Providing they confer sterile immunity, nonintegrative lentiviral vectors (NILV) hold promise as mass pediatric vaccine by meeting high safety standards. In this study, we have assessed the protective efficacy of NILV against malaria in a robust pre-clinical model. Mice were immunized with NILV encoding Plasmodium yoelii Circumsporozoite Protein (Py CSP) and challenged with sporozoites one month later. In two independent protective efficacy studies, 50% (37.5-62.5) of the animals were fully protected (p = 0.0072 and p = 0.0008 respectively when compared to naive mice). The remaining mice with detectable parasitized red blood cells exhibited a prolonged patency and reduced parasitemia. Moreover, protection was long-lasting with 42.8% sterile protection six months after the last immunization (p = 0.0042). Post-challenge CD8+ T cells to CSP, in contrast to anti-CSP antibodies, were associated with protection (r = -0.6615 and p = 0.0004 between the frequency of IFN-g secreting specific T cells in spleen and parasitemia). However, while NILV and RAS immunizations elicited comparable immunity to CSP, only RAS conferred 100% of sterile protection. Given that a better protection can be anticipated from a multi-antigen vaccine and an optimized vector design, NILV appear as a promising malaria vaccine.
A Small‐Molecule Agonist of the Wnt Signaling Pathway (2005)

Entwicklungsbiologie: Das 4,6-disubstituierte 2-Aminopyrimidin 1 erwies sich beim Screening kombinatorischer Bibliotheken als dosisabhängiger Agonist der Wnt-Signalübertragung. Kaulquappen aus mit 1 behandelten Embryonen zeigten schwere Schäden im Kopfbereich (rechts; links: ohne 1). Verbindung 1 scheint im Xenopus-Modell die Effekte eines Wnt-Liganden zu imitieren und könnte helfen, physiologische Prozesse aufzuklären, an denen Wnt mitwirkt.
ChemInform Abstract: Incorporation of Carbohydrates and Peptides into Large Triazine‐Based Screening Libraries Using Automated Parallel Synthesis (1998)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Comparison of Membrane Performance of PDMS-Based Membranes during Ethanol/Water Pervaporation and Fermentation Broth Pervaporation (2011)

In this study, fermentation broth pervaporation is compared to ethanol/water pervaporation. Laboratory unfilled en ZSM-5 filled PDMS membrane samples were used for this purpose. No significant changes were observed for the unfilled membrane, whereas water flux of the zeolite filled membrane was higher during fermentation broth pervaporation, which resulted in a lower permeate ethanol concentration. Subsequently this membrane was subjected to experiments with model ethanol/water mixtures in which the main fermentation by-products were individually added. It was found that carboxylic acids were responsible for the decreased membrane performance, their concentration rather than the nature of the acid itself determining the extent of membrane deterioration. An explanation was provided by interaction of the carboxylic acids with silanol end-groups of the zeolite surface rendering the membrane more hydrophilic. This was further confirmed by a decrease in water contact angle of the membrane after the experiments with acidic solutions. Membrane fouling could be avoided by increasing the pH to more neutral environments since dissociated ions hardly adsorb on zeolite particles.
In vitro reconstruction of a cartilage matrix granule network (1989)

The cartilage extracellular matrix contains electron-dense granules and fine filaments when studied electron microscopically after staining with ruthenium red. The matrix granules contain proteoglycans, while the filaments are thought to represent hyaluronic acid. In the present study partial extraction of proteoglycans from the cartilage prior to staining reduced the density of matrix components to allow visualization of a well-developed network involving the matrix granules and hyaluronic acid filaments. The matrix granules frequently had multiple filamentous attachments and the network appeared to be formed by intersecting filaments with the matrix granules at points of intersection. A similar network was created in Sepharose CL-2B beads when proteoglycans, link proteins, and hyaluronic acid were concentrated in the beads. Elimination of any one of these components resulted in failure to form a complete network. Purified proteoglycan monomers alone were sufficient to create matrix granules in the beads. Filaments were seen only when hyaluronic acid was added to the beads. The nature of the network suggests that some type of association between separate aggregates is occurring both within cartilage and within the Sepharose CL-2B beads.
ApoE2-associated hypertriglyceridemia is ameliorated by increased levels of apoA-V but unaffected by apoC-III deficiency (2020)

Apolipoprotein E2 (apoE2)-associated hyperlipidemia is characterized by a disturbed clearance of apoE2-enriched VLDL remnants. Because excess apoE2 inhibits LPL-mediated triglyceride (TG) hydrolysis in vitro, we investigated whether direct or indirect stimulation of LPL activity in vivo reduces the apoE2-associated hypertriglyceridemia. Here, we studied the role of LPL and two potent modifiers, the LPL inhibitor apoC-III and the LPL activator apoA-V, in APOE2-knockin (APOE2) mice. Injection of heparin in APOE2 mice reduced plasma TG by 53% and plasma total cholesterol (TC) by 18%. Adenovirus-mediated overexpression of LPL reduced plasma TG by 85% and TC by 40%. Both experiments indicate that the TG in apoE2-enriched particles is a suitable substrate for LPL. Indirect activation of LPL activity via deletion of Apoc3 in APOE2 mice did not affect plasma TG levels, whereas overexpression of Apoa5 in APOE2 mice did reduce plasma TG by 81% and plasma TC by 41%. In conclusion, the hypertriglyceridemia in APOE2 mice can be ameliorated by the direct activation of LPL activity. Indirect activation of LPL via overexpression of apoA-V does, whereas deletion of apoC-III does not, affect the plasma TGs in APOE2 mice. These data indicate that changes in apoA-V levels have a dominant effect over changes in apoC-III levels in the improvement of APOE2-associated hypertriglyceridemia.
Iron‑chromium mixed metal oxides catalyse the oxidative dehydrogenation of propane using carbon dioxide (2021)

The oxidative dehydrogenation of propane to propene using carbon dioxide is an attractive novel synthesis route to produce a key platform chemical. We investigated iron-chromia catalysts prepared by sol-gel. The mixed oxides showed improved specific surface area and a high concentration of acid and base sites and were found to be highly active catalysts. Although coking was observed over the catalysts, oxidative regeneration restored the majority of the initial activity over 5 consecutive cycles. The initial space time yield was amongst the most active Cr-based catalysts in the literature.