gebhart/patrepeval huggingface dataset:
SdiA, an Escherichia coli homologue of quorum‐sensing regulators, controls the expression of virulence factors in enterohaemorrhagic Escherichia coli O157:H7 (2000)

The quorum-sensing system in bacteria is a well-known regulatory system that controls gene expression in a cell density-dependent manner. A transcriptional regulator (LuxR homologue), signal synthase (LuxI homologue) and autoinducer (acyl homoserine lactone) are indispensable for this system in most Gram-negative bacteria. In this study, we found that SdiA, an Escherichia coli LuxR homologue, is a negative regulator of the expression of virulence factors EspD and intimin in enterohaemorrhagic E. coli (EHEC) O157:H7. The expression of EspD and intimin was inhibited at the RNA level upon SdiA overexpression. SdiA has a DNA-binding motif in its C-terminal part and can bind to the promoter regions of the esp and eae genes in vitro. Extracellular factors, which accumulate in culture supernatants of O157:H7 at the stationary phase of growth and inhibit EspD and intimin synthesis, bind to the N-terminal part of SdiA in vivo and in vitro. O157:H7 overproducing the N-terminal part of SdiA exhibited hypertranscription of EspD and intimin, suggesting that the overproduced N-terminal part had inhibited the activity of intact SdiA through titration of the extracellular factors. These results indicate that a quorum-sensing system including the SdiA protein controls colonization by O157:H7.
Modulation of Liver Inflammation and Fibrosis by Interleukin-37 (2021)

<b>Background and Aims:</b> Chronic inflammation induces liver fibrosis, cirrhosis and potentially liver cancer. Kupffer cells modulate hepatic stellate cells by secreting immunologically active proteins as TGF-β. TGF-β promotes liver fibrosis <i>via</i> the activation of Sma- and Mad-related protein 3. IL-37 broadly suppresses innate and adaptive immune responses. Intracellular IL-37 interacts with Smad3. We hypothesize that IL-37 downregulates the activation of hepatic Kupffer and stellate cells and interferes with the TGF-β signaling cascade to modulate liver fibrogenesis. <b>Methods:</b> The role of IL-37 on liver inflammation and fibrogenesis was assessed in three mouse models as well as isolated Kupffer- and stellate cells. Serum IL-37 was tested by ELISA in a clinical cohort and correlated with liver disease severity. <b>Results:</b> Transgene expression of IL-37 in mice extends survival, reduces hepatic damage, expression of early markers of fibrosis and histologically assessed liver fibrosis after bile duct ligation. IL-37tg mice were protected against CCl<sub>4</sub>-induced liver inflammation. Colitis-associated liver inflammation and fibrosis was less severe in IL-10 knockout IL-37tg mice. Spontaneous and LPS/TGF-β-induced cytokine release and profibrogenic gene expression was lower in HSC and KC isolated from IL-37tg mice and IL-37 overexpressing, IL-1β stimulated human LX-2 stellate cells. However, administration of recombinant human IL-37 did not modulate fibrosis pathways after BDL in mice, LX2 cells or murine HSCs. In a large clinical cohort, we observed a positive correlation of serum IL-37 levels with disease severity in liver cirrhosis. <b>Conclusions:</b> Predominantly intracellular IL-37 downregulates liver inflammation and fibrosis. The correlation of serum IL-37 with disease severity in cirrhosis suggests its potential as a novel target modulating the course of liver fibrosis.
Graphene 3D Architectures (2017)

A strategy has been developed to obtain ultralight chemically converted graphene aerogels with high compressibility. For practical applications, small graphene nanosheets usually need to be constructed or assembled into macroscopic materials. Leavening has been demonstrated to be an effective approach to transfer compact graphene structures into porous ones, by finding a proper leavening procedure. Template-guided methods are also effective in developing three-dimensional graphene architectures. The graphene layers contained a large amount of ripples and wrinkles, due to the difference in the thermal expansion coefficients between the nickel and graphene. To prevent the graphene network from collapsing during the etching of the nickel skeleton, a thin layer of poly(methyl methacrylate) was deposited on top of the graphene films as a support. The graphene-based structures were mechanically strong while maintaining high flexibility. Graphene-based materials in general have been employed to fabricate various sensing devices, because graphene nanosheets have a huge specific surface area and unique optoelectronic properties.
Neuromuscular Electrical Stimulation as a Method to Maximize the Beneficial Effects of Muscle Stem Cells Transplanted into Dystrophic Skeletal Muscle (2013)

Cellular therapy is a potential approach to improve the regenerative capacity of damaged or diseased skeletal muscle. However, its clinical use has often been limited by impaired donor cell survival, proliferation and differentiation following transplantation. Additionally, functional improvements after transplantation are all-too-often negligible. Because the host microenvironment plays an important role in the fate of transplanted cells, methods to modulate the microenvironment and guide donor cell behavior are warranted. The purpose of this study was to investigate whether the use of neuromuscular electrical stimulation (NMES) for 1 or 4 weeks following muscle-derived stem cell (MDSC) transplantation into dystrophic skeletal muscle can modulate the fate of donor cells and enhance their contribution to muscle regeneration and functional improvements. Animals submitted to 4 weeks of NMES after transplantation demonstrated a 2-fold increase in the number of dystrophin+ myofibers as compared to control transplanted muscles. These findings were concomitant with an increased vascularity in the MDSC+NMES group when compared to non-stimulated counterparts. Additionally, animals subjected to NMES (with or without MDSC transplantation) presented an increased maximal specific tetanic force when compared to controls. Although cell transplantation and/or the use of NMES resulted in no changes in fatigue resistance, the combination of both MDSC transplantation and NMES resulted in a faster recovery from fatigue, when compared to non-injected and non-stimulated counterparts. We conclude that NMES is a viable method to improve MDSC engraftment, enhance dystrophic muscle strength, and, in combination with MDSC transplantation, improve recovery from fatigue. These findings suggest that NMES may be a clinically-relevant adjunct approach for cell transplantation into skeletal muscle.
Applicability, safety, and biological activity of regulatory T cell therapy in liver transplantation (2019)

Regulatory T cells (Tregs) are a lymphocyte subset with intrinsic immunosuppressive properties that can be expanded in large numbers ex vivo and have been shown to prevent allograft rejection and promote tolerance in animal models. To investigate the safety, applicability, and biological activity of autologous Treg adoptive transfer in humans, we conducted an open-label, dose-escalation, Phase I clinical trial in liver transplantation. Patients were enrolled while awaiting liver transplantation or 6-12 months posttransplant. Circulating Tregs were isolated from blood or leukapheresis, expanded under good manufacturing practices (GMP) conditions, and administered intravenously at either 0.5-1 million Tregs/kg or 3-4.5 million Tregs/kg. The primary endpoint was the rate of dose- limiting toxicities occurring within 4 weeks of infusion. The applicability of the clinical protocol was poor unless patient recruitment was deferred until 6-12 months posttransplant. Thus, only 3 of the 17 patients who consented while awaiting liver transplantation were dosed. In contrast, all six patients who consented 6-12 months posttransplant received the cell infusion. Treg transfer was safe, transiently increased the pool of circulating Tregs and reduced anti-donor T cell responses. Our study opens the door to employing Treg immunotherapy to facilitate the reduction or complete discontinuation of immunosuppression following liver transplantation.
Surfaceome interrogation using an RNA-seq approach highlights leukemia initiating cell biomarkers in an LMO2 T cell transgenic model (2019)

The surfaceome is critical because surface proteins provide a gateway for internal signals and transfer of molecules into cells, and surfaceome differences can influence therapy response. We have used a surfaceome analysis method, based on comparing RNA-seq data between normal and abnormal cells (Surfaceome DataBase Mining or Surfaceome DBM), to identify sets of upregulated cell surface protein mRNAs in an LMO2-mediated T-ALL mouse model and corroborated by protein detection using antibodies. In this model the leukemia initiating cells (LICs) comprise pre-leukaemic, differentiation inhibited thymocytes allowing us to provide a profile of the LIC surfaceome in which GPR56, CD53 and CD59a are co-expressed with CD25. Implementation of cell surface interaction assays demonstrates fluid interaction of surface proteins and CD25 is only internalized when co-localized with other proteins. The Surfaceome DBM approach to analyse cancer cell surfaceomes is a way to find targetable surface biomarkers for clinical conditions where RNA-seq data from normal and abnormal cell are available.
NIR spectrometer using a Schottky photodetector enhanced by grating-based SPR (2016)

We present a near-infrared (NIR) spectrum measurement method using a Schottky photodetector enhanced by surface plasmon resonance (SPR). An Au grating was fabricated on an n-type silicon wafer to form a Schottky barrier and act as an SPR coupler. The resulting photodetector provides wavelength-selective photodetection depending on the SPR coupling angle. A matrix was pre-calculated to describe this characteristic. The spectrum was obtained from this matrix and the measured photocurrents at various SPR coupling angles. Light with single and multiple wavelengths was tested. Comparative measurements showed that our method is able to detect spectra with a wavelength resolution comparable to that of a commercial spectrometer.
Cancer-associated fibroblast-derived WNT2 increases tumor angiogenesis in colon cancer (2019)

WNT2 acts as a pro-angiogenic factor in placental vascularization and increases angiogenesis in liver sinusoidal endothelial cells (ECs) and other ECs. Increased WNT2 expression is detectable in many carcinomas and participates in tumor progression. In human colorectal cancer (CRC), WNT2 is selectively elevated in cancer-associated fibroblasts (CAFs), leading to increased invasion and metastasis. However, if there is a role for WNT2 in colon cancer, angiogenesis was not addressed so far. We demonstrate that WNT2 enhances EC migration/invasion, while it induces canonical WNT signaling in a small subset of cells. Knockdown of WNT2 in CAFs significantly reduced angiogenesis in a physiologically relevant assay, which allows precise assessment of key angiogenic properties. In line with these results, expression of WNT2 in otherwise WNT2-devoid skin fibroblasts led to increased angiogenesis. In CRC xenografts, WNT2 overexpression resulted in enhanced vessel density and tumor volume. Moreover, WNT2 expression correlates with vessel markers in human CRC. Secretome profiling of CAFs by mass spectrometry and cytokine arrays revealed that proteins associated with pro-angiogenic functions are elevated by WNT2. These included extracellular matrix molecules, ANG-2, IL-6, G-CSF, and PGF. The latter three increased angiogenesis. Thus, stromal-derived WNT2 elevates angiogenesis in CRC by shifting the balance towards pro-angiogenic signals.
Submicrosecond all-optical switching in neodymium-doped fiber (1994)

Sub-microsecond all—optical switching in neodymium-doped fiberR. W. Sadowski, M. J. F. Digonnet, R. H. Pantell and H. J. ShawEdward L. Ginzton LaboratoryStanford UniversityStanford, CA 94305ABSTRACTWe report on optically induced, sub-microsecond optical switching in an elliptical core neodymiumdoped, two-mode fiber. The improved response time of this switch, which is three orders of magnitude
Supplementary Figure S1-S6 from ER Stress Signaling Promotes the Survival of Cancer “Persister Cells” Tolerant to EGFR Tyrosine Kinase Inhibitors (2023)

&lt;p&gt;Figure S1 Preparation experiments for CRIPSR screening. Figure S2 Quality control of the result of CRISPR screening. Figure S3 Validation experiments with PC9 or HCC827 cells transduced sgRNAs targeting ufmylation. Figure S4 ER stress pathway response involve in the phenotypic change of the PC9 cells in the abcense of ufmylation. Figure S5 Inflammation pathway activation affect the status of cancer cells through modifying the apoptosis pathway. Figure S6 Bcl-xL dependency in the cells with increased ER stress&lt;/p&gt;
Solution-Processed Inorganic–Organic Hybrid Electron Injection Layer for Polymer Light-Emitting Devices (2012)

A lithium quinolate complex (Liq) has high solubility in polar solvents such as alcohols and can be spin-coated onto emitting polymers, resulting in a smooth surface morphology. A polymer light-emitting device fabricated with spin-coated Liq as an electron injection layer (EIL) exhibited a lower turn-on voltage and a higher efficiency than a device with spin-coated Cs₂CO₃ and a device with thermally evaporated Ca. The mixture of ZnO nanoparticles and Liq served as an efficient EIL, resulting in a lower driving voltage even in thick films (∼10 nm), and it did not require a high-temperature annealing process.
ChemInform Abstract: PREPARATION AND PROPERTIES OF DINITROGEN TRIMETHYLPHOSPHINE COMPLEXES OF MOLYBDENUM AND TUNGSTEN. 4. SYNTHESIS, CHEMICAL PROPERTIES, AND X‐RAY STRUCTURE OF CIS‐(MO(N2)2(PME3)4). THE CRYSTAL AND MOLECULAR STRUCTURES OF TRANS‐(MO(C2H4)2(PME3)4) AND TRANS,MER‐(MO(C2H4)2(CO)(PME3)3) (1983)

Abstract Die Darstellung des cis‐Bis‐[distickstoffl‐Komplexes (III) erfolgt durch Reduktion des Tris‐[phosphin]‐Mo(III)‐Ana‐ logen (II) von Trichloro‐tris‐[THF]‐Mo(III) (I) mit dispergiertem Na und nachfolgender Trimethylphosphin‐Zugabe.
Isolation of Cytotoxic Metabolites from Targeted Peruvian Amazonian Medicinal Plants (2007)

The antiproliferative bioassay-guided fractionation of five Peruvian plants, Doliocarpus dentatus, Picramnia sellowii, Strychnos mitscherlichii, Iryanthera juruensis, and Croton alnifolius, led to the isolation and identification of their different major cytotoxic constituents, betulinic acid (1), nataloe-emodin (2), bisnordihydrotoxyferine (4), 2',4'-dihydroxy-6'-methoxy-3,4-methylenedioxydihydrochalcone (5), and 2',4'-dihydroxy-4,6'-dimethoxydihydrochalcone (6) and 12-O-tetradecanoylphorbol-13-acetate (7), respectively. Eight human tumor cell lines and two nontumorigenic cell lines were used in this investigation. Their in vitro activity against Mycobacterium tuberculosis is also reported.
Targeting an evolutionarily conserved “E-L-L” motif in the spike protein to develop a small molecule fusion inhibitor against SARS-CoV-2 (2022)

As newer variants of SARS-CoV-2 continue to pose major threats to global human health and economy, identifying novel druggable antiviral targets is the key towards sustenance. Here, we identify an evolutionary conserved E-L-L motif present within the HR2 domain of all human and non-human coronavirus spike (S) proteins that play a crucial role in stabilizing the post-fusion six-helix bundle (6-HB) structure and thus, fusion-mediated viral entry. Mutations within this motif reduce the fusogenicity of the S protein without affecting its stability or membrane localization. We found that posaconazole, an FDA-approved drug, binds to this E-L-L motif resulting in effective inhibition of SARS-CoV-2 infection in cells. While posaconazole exhibits high efficacy towards blocking S protein-mediated viral entry, mutations within the E-L-L motif rendered the protein completely resistant to the drug, establishing its specificity towards this motif. Our data demonstrate that posaconazole restricts early stages of infection through specific inhibition of membrane fusion and viral genome release into the host cell and is equally effective towards all major variants of concerns of SARS-CoV-2 including beta, kappa, delta, and omicron. Together, we show that this conserved essential E-L-L motif is an ideal target for the development of prophylactic and therapeutic interventions against SARS-CoV-2.
A computer simulation of an induction heating system (1991)

A method for the design and analysis of induction heating systems is presented. The method entails the simulation of system performance using an equivalent circuit approach. Equivalent circuit models are obtained for the three parts of an induction heating system: the power source, the impedance matching circuit, and the load. These models are combined into a system model which is analyzed using the advanced continuous simulation language (ACSL). This approach is applied to an existing system, and the predicted performance is in close agreement with measured data.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Retraction of “Fabrication of Tantalum and Nitrogen Codoped ZnO (Ta, N-ZnO) Thin Films Using the Electrospay: Twin Applications as an Excellent Transparent Electrode and a Field Emitter” (2015)

ADVERTISEMENT RETURN TO ISSUEPREVRetractionNEXTORIGINAL ARTICLEThis notice is a retraction.Retraction of "Fabrication of Tantalum and Nitrogen Codoped ZnO (Ta, N-ZnO) Thin Films Using the Electrospay: Twin Applications as an Excellent Transparent Electrode and a Field Emitter"Khalid Mahmood, Seung Bin Park*, and Hyung Jin SungCite this: ACS Appl. Mater. Interfaces 2015, 7, 46, 26005Publication Date (Web):November 12, 2015Publication History Published online12 November 2015Published inissue 25 November 2015https://pubs.acs.org/doi/10.1021/acsami.5b10101https://doi.org/10.1021/acsami.5b10101retractionACS PublicationsCopyright © 2015 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views1619Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (108 KB) Get e-Alertsclose Get e-Alerts
HST-1/FGF-4 stimulates proliferation of megakaryocyte progenitors synergistically and promotes megakaryocyte maturation. (1996)

Megakaryocyte (MK) development is dependent on the complex interaction of MK progenitors, various cytokines and stromal elements. We previously reported that an injection of replication-deficient adenovirus containing HST-1/FGF-4 cDNA (Adex1HST-1) into mice caused a twofold increase in peripheral platelet count for 30 days without any other hematological or histological abnormality. In the present study using Adex1HST-1-infected human megakaryocytic Dami cells, we demonstrated for the first time that HST-1/FGF-4 promoted MK maturation, inducing increases in DNA ploidy, cytoplasmic and membrane maturation, and platelet-like particle release. Moreover, HST-1/FGF-4 acted on megakaryocytic cells to induce secretion of IL-6 and TNF-alpha, and increased adhesion of megakaryocytic cells to human endothelial cells primarily via VLA-4 and LFA-1 molecules; both mechanisms have been shown to lead to MK maturation. We also showed that HST-1/FGF-4 stimulates the proliferation of MK progenitors not alone but synergistically with IL-3 via IL-6 and with c-mpl ligand (thrombopoietin) not via IL-6. This result supports the hypothesis of the presence of two distinct populations of MK progenitors: IL-3-dependent and Tpo-dependent. All these results suggest that HST-1/FGF-4 can regulate MK development not only as an MK potentiating factor, but also as an inducer of cytokine secretion from MK, and as a modulator of adhesive interactions with endothelial cells.
Materials analysis and device optimization of CuInSe/sub 2/ solar cells: Annual subcontract report, 16 January 1987--15 January 1988 (1989)

This report presents results and conclusions from the first year of research on CuInSe/sub 2/ thin-film solar cells. The research was carried out in two tasks: materials preparation and analysis and high-efficiency solar cell fabrication and analysis. CuInSe/sub 2/ thin films were prepared by vacuum evaporation of elemental Cu, In, and Se onto heated substrates. Systematic studies of the effects of incident flux and substrate temperature on CuInSe/sub 2/ film composition and morphology are presented. Mirror-smooth CuInSe/sub 2/ films were prepared using a newly developed aqueous bromine solution as chemical polishing etch. Heterojunctions photovoltaic devices with (CdZn)S, ITO/(CdZn)S, ZnO, and ZnO/(CdZn)S window layers were fabricated and analyzed. The dominant recombination mechanism in high-efficiency CuInSe/sub 2/ devices was found to be space-charge recombination in the CuInSe/sub 2/. Optimized one-square-centimeter CuInSe/sub 2//(CdZn)S solar cells with efficiencies greater than 10% were fabricated. 17 refs.
Exploring the use of submarine cables and related technologies (2003)

Outcomes from real‐time and long‐term observations using submarine cables in the Earth and ocean sciences have been actively reported and published in recent years, and scientists are now looking at advanced, next‐generation observation technologies. The third International Workshop on Scientific Use of Submarine Cables and Related Technologies (SSC '03) was held last June at the University of Tokyo to explore some of these. The first and second workshops were held in 1990 in Hawaii and in 1997 in Okinawa, respectively. The discussions from these generated experiments in the use of decommissioned telecommunication cables, and conceptual development or designs for multidisciplinary sea floor observatories, both in the United States and in Japan. The third workshop was intended to promote better mutual understanding between scientists and engineers, and a broader perspective on future submarine observations. Thus, the objectives were to gather both Earth and ocean scientists and engineers from multiple disciplines and encourage them to think objectively about necessary future collaboration and cooperation as a team for the advancement of ocean floor observations.
Computational simulation of pacifier deformation and interaction with the palate (2021)

A nonlinear transient dynamic finite element model can simulate the mechanical behavior of a pacifier and its interaction with the tongue and contact with the palate subject to NNS. Quantitative results predicting deformation, strain, stress, contact force, and contact area can be used in comparative studies to provide insight on how pacifiers cause changes in dental, orthognathic, and facial development.
A low noise and low cost YIG-oscillator in the frequency range from 2.2 GHz to 6.4 GHz for use in a high end microwave synthesizer (2002)

A low noise and low cost YIG-oscillator which is easy to stabilize within a phase-locked-loop has been developed. In order to achieve a good phase noise performance it has been simulated using only the linear scattering parameters of the transistors. Measurements on self-built prototype circuits have been made and show a good accordance with the calculation.