gebhart/patrepeval huggingface dataset:
Reversible cryoactivation of recombinant human prorenin. (1992)

Cleavage of prorenin's prosegment causes irreversible formation of renin. In contrast, renin activity is reversibly exposed when prorenin is acidified to pH 3.3. Nonetheless, acidification of plasma results in irreversible activation of prorenin, because endogenous proteases cleave the prosegment of acid-activated prorenin. Chilling of plasma results in irreversible cryoactivation of prorenin. In this study we investigated whether cryoactivation of purified prorenin is reversible. The intrinsic renin activity of recombinant human prorenin was measured by an enzyme kinetic assay using partially purified human angiotensinogen as substrate. Results are expressed as a percent (mean +/- S.E.) of the maximal activity exposed after limited proteolysis by trypsin. The intrinsic renin activity of two pools (0.3 and 0.06 Goldblatt units/ml) was 1.5% +/- 0.3 and 1.2% +/- 0.6 at 37 degrees C. Activity increased to 19% +/- 0.3 and 26% +/- 0.5 after incubation at 0 degrees C and to 5.4% +/- 0.5 and 2.1% +/- 1.2 at room temperature. Cryoactivation did not occur in buffers containing more than 1 M NaCl. It took 8 min at 37 degrees C or 180 min at room temperature for cryoactivated prorenin to lose half of its intrinsic renin activity. It took 48 and 26 h, respectively, at 0 degree C for the two pools of prorenin at 37 degrees C to regain half of their maximum intrinsic activity at 0 degrees C. A direct immunoradiometric assay that detects active renin but not prorenin was able to detect cryoactivated prorenin. These results show that human prorenin can be reversibly cryoactivated in buffers of low ionic strength and has greater intrinsic activity at room temperature than at 37 degrees C.
Allogeneic stem cell transplantation for renal cell carcinoma (2001)

Although the prognosis for patients with metastatic kidney cancer remains poor, a number of promising immunotherapeutic approaches for the treatment of metastatic disease have been developed over the past decade. The response of some patients to cytokines such as interleukin-2 and interferon-alpha, and more recently, vaccination with dendritic cell/tumor fusions has laid the ground work for ongoing immune-based investigational approaches. Allogeneic stem cell transplantation is a potent form of immunotherapy capable of delivering potentially curative immune-mediated anti-tumor effects against a number of different hematological malignancies. Knowledge of renal cell carcinoma's unusual susceptibility to immune attack has led to the hypothesis that tumor rejection, mediated through immunocompetent donor T-cells, might be generated against this solid tumor following the transplantation of an allogeneic immune system. Although clinical trials are early and ongoing, the recent observation of metastatic disease regression following non-myeloablative stem cell transplantation has identified renal cell carcinoma as being susceptible to a graft-versus-tumor effect. Disease responses following such therapy have ranged from partial to complete and have been observed even in patients who have failed conventional cytokine based strategies. This article reviews the design, methodology and early clinical results of studies investigating the use of allogeneic stem cell transplantation in metastatic renal cell carcinoma.
Representation Learning-Driven Fully Automated Framework for the Inverse Design of Frequency-Selective Surfaces (2023)

Frequency-selective surfaces (FSSs) refer to planar structures that behave with specific electromagnetic (EM) responses within a frequency range and are widely applied in wireless propagation systems. Given the fact that different EM responses correspond to distinguished topologies, conventional inverse design methods of FSSs are usually labor-intensive, as they rely on experienced human engineers to determine the topology and then rationally tune its structures. There have been great attempts using optimization algorithms (e.g., genetic algorithms) or machine learning to automate the second tuning stage after the initial EM topologies are determined by human engineers. However, the first topology selection stage still requires engagements with experienced engineers. This article proposes a fully automated framework for the inverse design of FSSs. We achieved a fully automated inverse design by establishing a machine-friendly mapping flow. The mapping flow derives its continuity and compactness from representation learning, which enables both autoselection of the topology and autoevolution of the unit cell based on the topology. The autoselection stage automatically determines the appropriate topology by compressing the EM constraints through the principal component analysis (PCA) and classifying the topology using the support vector machine (SVM). Afterward, the autoevolution system can efficiently evolve until it yields an optimal unit cell. We developed a self-monitor strategy to control the evolution and maximize the evolution efficiency by adaptively tuning the three modules within the autoevolution system. We validated the presented framework with four FSS designs. The results proved its potential as a highly efficient fully automated tool for the inverse design of FSSs.
The influence of mestranol and chlormadinone given separately or in combination on the effects evoked by a high-fat diet in female rabbits. (1990)

The effect of mestranol and chlormadinone, the hormones contained in oral contraceptives: on lipid metabolism, some hemostatic parameters and on morphology of aorta, liver and heart was tested after separate or combined administration to mongrel female rabbits fed on a normal or high-fat diet (HFD). HFD administration led to typical atheromatous changes in the aortal intima. The hormones given together with HFD attenuated the augmentation of lipid serum level and the extent of atheromatous plaques in the intima. The percent content of alpha-lipoproteins was significantly lower in groups receiving HFD with or without the hormones than in the controls, but it was higher than in controls in the rabbits receiving the hormones alone. Chlormadinone given together with HFD potentiated atheromatous aortal changes, which occurred not only in the intima but also in the media. Mestranol given together with HFD significantly counteracted the development of atheromatous changes. Female rabbits receiving mestranol and chlormadinone displayed a significant increase in fibrinogen content, while those receiving mestranol showed an increased time of fibrinolysis. HFD produced a hepatic damage, which was augmented in rabbits concomitantly receiving the hormones. The hormones alone also displayed some, a less pronounced hepatotoxic action.
Nonlinear Electrical Spin Conversion in a Biased Ferromagnetic Tunnel Contact (2018)

The electrical detection of spin accumulation in nonmagnetic materials is required for the operation of many spintronic devices. Typically, detection is achieved via a tunnel contact with a ferromagnetic electrode. Spin conversion in a magnetic tunnel contact is conventionally described using linear transport equations---but here the authors demonstrate that electrical spin detection in a biased ferromagnetic tunnel contact is inherently $n\phantom{\rule{0}{0ex}}o\phantom{\rule{0}{0ex}}n\phantom{\rule{0}{0ex}}l\phantom{\rule{0}{0ex}}i\phantom{\rule{0}{0ex}}n\phantom{\rule{0}{0ex}}e\phantom{\rule{0}{0ex}}a\phantom{\rule{0}{0ex}}r$. This explains a multitude of puzzling spin-transport data, including two-terminal magnetoresistance data, and truly changes our view of spintronic device physics.
Abstract 4819: Sacituzumab Govitecan (IMMU-132) in uterine serous carcinoma (2019)

Objective: Uterine serous carcinoma (USC) is an aggressive variant of endometrial cancer with poor prognosis. Sacituzumab govitecan (IMMU-132) is a novel antibody-drug conjugate (ADC) targeting trophoblast antigen 2 (Trop-2), a cell surface glycoprotein highly expressed in many epithelial tumors including USC, to deliver SN-38, the active metabolite of irinotecan. The objective of this study was to preclinically evaluate the efficacy of IMMU-132 against primary USC cell lines and xenografts.Methods: Trop-2 expression in primary tumor cell lines and USC cell viability after exposure to IMMU-132 ADC (hRS7-CL2A-SN-38), non-targeting control ADC (h679-CL2A-SN-38), and naked antibody hRS7 IgG were evaluated using RT-PCR and flow-cytometry-based-assays. Antibody-dependent-cell-cytotoxicity (ADCC) against Trop-2+ and Trop-2- USC cell lines was evaluated in vitro using 4 hr Chromium release assays. Finally, in vivo activity of Sacituzumab govitecan was tested against Trop-2+ USC xenografts by 3 twice-a-week retro-orbital injection of 500 μg of IMMU-132, control-ADC, and hRS7 naked-IgG.Results: Overexpression of Trop-2 was detected in 67% (8 out of 12) of primary USC cell lines. Primary tumors overexpressing Trop-2 were significantly more sensitive (ie, lower IC50) to IMMU-132 (hRS7-CL2A-SN-38) when compared to control ADC (h679-CL2A-SN-38). Both sacituzumab govitecan (IMMU-132) and the naked antibody hRS7 induced high level of ADCC against Trop2+ USC cell lines while no cytotoxicity was detected against Trop-2 negative tumors. In vivo experiments comparing IMMU-132 activity to control ADC and hRS7 showed a dramatically improved tumor suppression and increased survival in IMMU-132 treated mice when compared to controls (P = 0.0001 and P = 0.0002, respectively).Conclusion: IMMU-132 demonstrated remarkable antitumor activity against biologically aggressive USC overexpressing Trop-2. Our preclinical results combined with the dramatic clinical response recently reported in an USC patient treated with IMMU-132 (https://doi.org/10.1016/j.gore.2018.05.009) strongly supports further clinical development of sacituzumab govitecan in USC patients with advanced/recurrent disease. (ie, clinical trial IND 140394). Furthermore, due to a cleavable linker that can cause bystander effect, sacituzumab govitecan could be also effective in tumors with heterogenous TROP-2 expression.Citation Format: Salvatore Lopez, Chanhee Han, Burak Zeybek, Elena Bonazzoli, Anna Bianchi, Paola Manara, Stefania Bellone, Aranzazu Manzano, Emanuele Perrone, Luca Zammataro, Gary Altwerger, Alessandro D. Santin. Sacituzumab Govitecan (IMMU-132) in uterine serous carcinoma [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 4819.
A study of HTLV-I and its associated risk factors in Trinidad and Tobago. (1990)

Seroprevalence of human T-lymphotropic virus type 1 (HTLV-I) among a sample of persons selected from a government register of businesses in Trinidad was 3.2% in 1,025 persons of African descent compared to 0.2% among 487 persons of Asian descent and 0% among 46 persons of European-descent. In Tobago, from a coastal village, among persons of African ancestry ascertained as part of a cardiovascular survey, the rate was 11.4%, which was significantly higher when corrected for age and race than the rate in Trinidad. The seroprevalence rate of antibodies to hepatitis A and B was also significantly elevated in Tobago compared to Trinidad. HTLV-I seroprevalence rates were higher in females than males while hepatitis A and B rates were not significantly different in the two sexes. For males, age was a significant determinant of HTLV-I seropositivity, while for females, age, markers of poor sanitation, and hepatitis B were each independently linked to HTLV-I seropositivity. The frequent occurrence of multiple infectious exposures in persons of lower socioeconomic circumstances in this tropical environment may result in immune activation that heightens susceptibility to HTLV-I infection.
Structural basis for the slow photocycle and late proton release in Acetabularia rhodopsin I from the marine plant Acetabularia acetabulum (2015)

Although many crystal structures of microbial rhodopsins have been solved, those with sufficient resolution to identify the functional water molecules are very limited. In this study, the Acetabularia rhodopsin I (ARI) protein derived from the marine alga A. acetabulum was synthesized on a large scale by the Escherichia coli cell-free membrane-protein production method, and crystal structures of ARI were determined at the second highest (1.52-1.80 Å) resolution for a microbial rhodopsin, following bacteriorhodopsin (BR). Examinations of the photochemical properties of ARI revealed that the photocycle of ARI is slower than that of BR and that its proton-transfer reactions are different from those of BR. In the present structures, a large cavity containing numerous water molecules exists on the extracellular side of ARI, explaining the relatively low pKa of Glu206(ARI), which cannot function as an initial proton-releasing residue at any pH. An interhelical hydrogen bond exists between Leu97(ARI) and Tyr221(ARI) on the cytoplasmic side, which facilitates the slow photocycle and regulates the pKa of Asp100(ARI), a potential proton donor to the Schiff base, in the dark state.
Ultra Stable Molecular Sensors by Submicron Referencing and Why They Should Be Interrogated by Optical Diffraction—Part II. Experimental Demonstration (2020)

Label-free optical biosensors are an invaluable tool for molecular interaction analysis. Over the past 30 years, refractometric biosensors and, in particular, surface plasmon resonance have matured to the <i>de facto</i> standard of this field despite a significant cross reactivity to environmental and experimental noise sources. In this paper, we demonstrate that sensors that apply the spatial affinity lock-in principle (part I) and perform readout by diffraction overcome the drawbacks of established refractometric biosensors. We show this with a direct comparison of the cover refractive index jump sensitivity as well as the surface mass resolution of an unstabilized diffractometric biosensor with a state-of-the-art Biacore 8k. A combined refractometric diffractometric biosensor demonstrates that a refractometric sensor requires a much higher measurement precision than the diffractometric to achieve the same resolution. In a conceptual and quantitative discussion, we elucidate the physical reasons behind and define the figure of merit of diffractometric biosensors. Because low-precision unstabilized diffractometric devices achieve the same resolution as bulky stabilized refractometric sensors, we believe that label-free optical sensors might soon move beyond the drug discovery lab as miniaturized, mass-produced environmental/medical sensors. In fact, combined with the right surface chemistry and recognition element, they might even bring the senses of smell/taste to our smart devices.
Diagnostic methods for a high efficiency exhaust aftertreatment system (2023)

A method includes acquiring nitrogen oxide (NOx) data indicative of a first amount of NOx in an exhaust flow exiting an engine and a second amount of NOx in the exhaust flow exiting an exhaust aftertreatment system coupled to the engine where the exhaust aftertreatment system including a selective catalytic reduction (SCR) system including a SCR catalyst; determining a NOx conversion efficiency fault is present within the exhaust aftertreatment system based on the first amount of NOx and the second amount of NOx; monitoring an actual amount of NOx in the exhaust flow downstream of the SCR catalyst; determining an expected amount of NOx downstream of the SCR catalyst; and determining the SCR catalyst is responsible for the NOx conversion efficiency fault in response to the actual amount of NOx differing from the expected amount of NOx by more than a threshold amount.
Algorithms and performance analysis for speculative computation in discrete-event simulation (2000)

Discrete-event simulation is used extensively in the design, modeling, and analysis of systems. The execution of discrete-event simulations on parallel computing platforms holds the promise of significantly improved performance. However, the widespread realization of high performance parallel simulation has yet to be achieved. This dissertation investigates the use of speculative computation to improve the performance of synchronous parallel simulation algorithms. The investigation comprises three components: empirical studies, algorithmic development, and analytic performance modeling. 
The empirical studies investigate the following questions. First, under what circumstances does speculative computation provide performance gains in a realistic discrete-event simulation application and environment. Second, what is the performance impact of partitioning on a parallel simulation implementation. Third, what is the long term stability of the computational workload and communications volume for a simulation application. 
A new algorithm is proposed for use in speculative simulation, called predictive optimism. Here, the past history of activity within the simulation is used to make predictions as to future activity. This has the potential to improve the accuracy of speculative decisions that are made during the execution of the simulation, thereby improving overall performance. 
Finally, to investigate the performance implications of predictive optimism, and speculative computation in general, an analytic performance model of synchronous iterative algorithms is extended to incorporate the effects of speculative computation. This model is validated using a pair of simulation applications, and is used to quantify the conditions under which speculative computation techniques provide sufficient performance benefits to justify their implementation and deployment.
Clinical Performance of the Consensus Immunoscore in Colon Cancer in the Asian Population from the Multicenter International SITC Study (2022)

BACKGROUND: In this study, we evaluated the prognostic value of Immunoscore in patients with stage I−III colon cancer (CC) in the Asian population. These patients were originally included in an international study led by the Society for Immunotherapy of Cancer (SITC) on 2681 patients with AJCC/UICC-TNM stages I−III CC. METHODS: CD3+ and cytotoxic CD8+ T-lymphocyte densities were quantified in the tumor and invasive margin by digital pathology. The association of Immunoscore with prognosis was evaluated for time to recurrence (TTR), disease-free survival (DFS), and overall survival (OS). RESULTS: Immunoscore stratified Asian patients (n = 423) into different risk categories and was not impacted by age. Recurrence-free rates at 3 years were 78.5%, 85.2%, and 98.3% for a Low, Intermediate, and High Immunoscore, respectively (HR[Low-vs-High] = 7.26 (95% CI 1.75−30.19); p = 0.0064). A High Immunoscore showed a significant association with prolonged TTR, OS, and DFS (p < 0.05). In Cox multivariable analysis stratified by center, Immunoscore association with TTR was independent (HR[Low-vs-Int+High] = 2.22 (95% CI 1.10−4.55) p = 0.0269) of the patient’s gender, T-stage, N-stage, sidedness, and MSI status. A significant association of a High Immunoscore with prolonged TTR was also found among MSS (HR[Low-vs-Int+High] = 4.58 (95% CI 2.27−9.23); p ≤ 0.0001), stage II (HR[Low-vs-Int+High] = 2.72 (95% CI 1.35−5.51); p = 0.0052), low-risk stage-II (HR[Low-vs-Int+High] = 2.62 (95% CI 1.21−5.68); p = 0.0146), and high-risk stage II patients (HR[Low-vs-Int+High] = 3.11 (95% CI 1.39−6.91); p = 0.0055). CONCLUSION: A High Immunoscore is significantly associated with the prolonged survival of CC patients within the Asian population.
The 100 µm length scale in the microbial ocean (2015)

Marine microorganisms live and interact at the microscale. Yet, in a field traditionally dominated by human-scale sampling approaches, we are only just beginning to develop an appreciation for the importance of microbial microscale processes. Here, I propose that the 100 m length scale is a useful yardstick to measure and develop an intuition for this rich microscale world, because it characterizes a range of physical, chemical and biological processes that occur in a microbe's quotidian journey through the sea.
New Proton Exchange Membranes for Direct Methanol Fuel Cells (2005)

Abstract : This three-year research program studied wholly aromatic, optionally fluorinated copolymers with the goal of establishing fundamental data about the complex relationships between PEM chemical structure, morphology, transport properties, and the performance of the DMFC fuel cell membranes. Accomplishments included the generation of lower permeability, high-power density PEM systems. Under ARO funding, we were also successful in scaling the ion containing comonomer in a single step (at the kilogram quantity or higher) as well as demonstrating viable routes for the synthesis of the desired copolymers. This funding also contributed to developing materials for the electrode catalyst layers that show competitive advantages over Nafion. MEAs were prepared, which were characterized by voltage current curves (primarily at LANL) and 300 hours stability at 80 degrees C was demonstrated.
Regulation of epidermal growth factor receptor number and phosphorylation by fasting in rat liver. (1986)

The binding of 125I-epidermal growth factor (EGF) to microsomal membrane preparations from the livers of rats fasted for 72 h or fed control or high carbohydrate diets was examined to determine whether alterations in nutrient intake could affect the EGF receptor system. Fasted rats had 40-50% less membrane binding than did control or carbohydrate-fed rats. Scatchard analysis of the binding data indicated that the decrease in EGF binding in fasted rats was due to a decrease in receptor number with no change in receptor affinity. Cross-linking of 125I-EGF to EGF receptors with disuccinimidyl suberate revealed specific binding of a Mr 170,000 protein, which was diminished by approximately 75% in fasting, and a Mr = 150,000 protein, which accounted for 40-50% of the total labeling in the control and carbohydrate-fed rats and which was relatively unchanged by fasting. The sum of the labeling of the 2 bands was reduced by approximately 40% in fasting and is consistent with the reduction in EGF binding detected by Scatchard analysis. EGF stimulated a 1.5-3-fold increase in 32P incorporation into one major protein of 170 kDa in all 3 groups. Basal and EGF-stimulated autophosphorylation of 170 kDa, when normalized for protein, was 75% lower in membranes from fasted animals, compared to those from control or carbohydrate-fed rats. The comparable reduction of 125I-EGF binding to, and 32P incorporation into, the 170-kDa EGF receptor protein suggested that kinase activity/receptor was unaffected by fasting. Moreover, EGF receptor kinase activity in the 3 groups was comparable for an exogenous substrate, as judged by equal basal and EGF-stimulated phosphorylation of Val5-angiotensin II, when normalized for total EGF-binding capacity. These results suggest that fasting regulates EGF receptor kinase activity primarily by regulation of the number of hepatic EGF receptors. The possibility exists that some in vivo effects of fasting may be mediated by a reduction in EGF receptor levels.
Intraoperative evaluation using mobile computed tomography in anterior cervical decompression with floating method for massive ossification of the posterior longitudinal ligament (2017)

Intraoperative CT imaging was effective to avoid insufficient decompression following ADF with the floating method for massive OPLL. We also consider that the intraoperative three-dimensional imaging is helpful for providing informative feedback to surgeons to improve performance in skill-intensive surgeries such as ADF with the floating method.