gebhart/patrepeval huggingface dataset:
Immunoglobulin G-dependent enhancement of the infection with Coxsackievirus B4 in a murine system (2016)

It was demonstrated that specific IgG can enhance the infection with CV-B4, in vitro, in the human system. This enhancement could be involved in the pathophysiology of CV-B4 induced diseases. To investigate further the role of enhancing IgG in the infection with CV-B4 E2 in vivo, animal models are needed. Therefore, it was decided to assess whether inoculation of CV-B4 E2 to mice results in the appearance of IgG able to enhance the infection with this virus. Swiss albino mice were inoculated with CV-B4 E2 intraperitoneally. Serum samples were obtained from tail vein blood collected from day 0 to day 80 p.i. IgG were isolated by Protein G affinity chromatography. Seroneutralisation assays were carried out. In total murine spleen cells cultures inoculated with CV-B4 E2 mixed with various dilutions of serum or IgG samples, the enhancing activity was assayed through i) the antiviral activity titer of supernatants ii) the detection of intracellular viral RNA by RT-PCR iii) the level of infectious particles in supernatants. In most serum samples (76/105), neutralizing and enhancing activities were detected peaking between days 14 and 30 p.i and were higher in sera from mice inoculated with 2.10(6) TCID50 units than with lower doses. The enhancing activity was due to the IgG-enriched fraction of serum from CV-B4 E2 infected animals but not from control animals. These data show that IgG from immune mice can enhance the infection of splenocytes with CV-B4 E2 in vitro and open the way to explore whether such an enhancing activity can play a role in vivo.
Deficiency in mycolipenate- and mycosanoate-derived acyltrehaloses enhances early interactions of Mycobacterium tuberculosis with host cells (2003)

Lipids that are uniquely found in the cell envelope of pathogenic mycobacteria, such as those containing multiple methyl-branched long-chain fatty acids, have long been thought to play a role in host-pathogen interactions. The recent construction by Dubey et al. (2002) Mol Microbiol 45: 1451-1459, of a Mycobacterium tuberculosis mutant that is deficient in the synthesis of the di- and tri-methylbranched fatty acids, mycolipenates and mycosanoates, found in some forms of diacyltrehaloses (DAT) and polyacyltrehaloses (PAT) provided the opportunity to assess the contribution of these complex lipids to pathogenesis directly. We provide evidence that DAT/PAT deficiency affects the surface global composition of the mycobacterial cell envelope improving the efficiency with which M. tuberculosis binds to and enters phagocytic and non-phagocytic host cells. Interestingly, this property did not affect the overall replication and persistence of the tubercle bacillus in the lungs, spleen and liver of mice infected via the respiratory or intravenous route.
Regulation of Hormone Responses in Prostate Cancer by BAGIL (2003)

Abstract : Androgen ablation therapy represents the principal treatment for metastatic prostate cancer. However, nearly all tumors eventually relapse as hormone-refractory disease, indicating a need to better understand how the androgen receptor (AR) functions. In this project, we studied the expression and function of an AR-binding protein discovered in our laboratory, BAG1L. We showed that BAG1L becomes over-expressed in many prostate cancers. We determined that BAG1L potentiates the actions of AR, lowering concentrations of dihydrotestosterone needed to activate AR and rendering the AR resistant to anti-androgen drugs. We mapped the domains in BAG1L required for this activity, and generated transgenic mice over-expressing BAG1L in the prostate for future use in studying effects of BAG1L on AR in vivo. Finally, we performed work showing that BAG1L also interacts with the Vitamin D Receptor (VDR) and potentiates the growth suppressive and pro-apoptotic effects of VDR- ligands in prostate cancer cell lines in culture. Taken together, these observations suggest that prostate cancers containing high levels of BAG1L are less likely to respond favorably to anti-androgen therapy (due to their decreased sensitivity to anti-androgens), but if these tumors express VDR, then they may be excellent candidates for Vitamin-D-based therapies (due to their increased sensitivity to Vitamin D and synthetic Vitamin D analogs).
Discovery of a Novel Series of Potent and Selective Substrate-Based Inhibitors of p60c-src Protein Tyrosine Kinase:  Conformational and Topographical Constraints in Peptide Design (1998)

On the basis of the efficient substrate for p60c-src protein tyrosine kinase (PTK) YIYGSFK-NH2 (1) (Km = 55 microM) obtained by combinatorial methods, we have designed and synthesized a series of conformationally and topographically constrained substrate-based peptide inhibitors of this enzyme, which showed IC50 values in the low-micromolar range (1-3 microM). A "rotamer scan" was performed by introducing the four stereoisomers of beta-Me(2')Nal in the postulated interaction site of the peptide inhibitor 23(IC50 = 1.6 microM). This substitution led to selective and potent inhibitors of p60c-src PTK; however, no substantial difference in potency was observed among them. This and the results of the "stereochemical scan" performed at residues 2 and 7 of 3 (peptides 19-21), which form the disulfide bond, may suggest that the enzyme active site does not have rigid topographic requirements and thus is able to achieve important conformational changes to bind the ligand as long as the pharmacophore pattern in the inhibitor is conserved. Two new potent iodo-containing nonphosphorylatable tyrosine analogues were also incorporated into our lead inhibitory sequence 23, producing the most potent inhibitors for p60c-src PTK identified thus far in our studies. Compounds 29 and 30 exhibit IC50 values of 0.13 and 0.54 microM, respectively. Peptide 29 is 420-fold more potent than the parent peptide 1. Selectivity studies of peptides 23-30 toward p60c-src, Lyn, and Lck PTK showed in general high Lyn/Src and moderate Lck/Src selectivity ratios. We found that the chi1 space constraints of the specialized amino acids, introduced at position 3 of the peptide lead 23, were not as important as the configuration of the Calpha of that residue to recognize the subtle chemical environment surrounding the active site of Src and Lck PTK, as reflected on the obtained Lck/Src selectivity ratios.
Abstract 15677: An Ascending Ramp First Phase Biphasic Shock Defibrillates With Lower Energy and Causes Markedly Less Damage (2016)

Introduction: ICD shocks damage the heart and may increase mortality. Hypothesis: We tested the hypothesis that a biphasic shock with an ascending ramp as first phase (RAMP, Fig A) causes less damage than a standard biphasic truncated exponential shock (BTE, Fig B). Methods: Either a 20J RAMP or a 25J BTE was delivered through right ventricular (RV) and SVC electrodes in pigs. To be considered a successful experiment, the waveform had to defibrillate 2 VF episodes 5 min apart. Six successes were required for each waveform. A bipolar recording between a tip lead electrode and the RV coil was made for STT changes. Blood samples were drawn before the first shock and hourly for 6 hours after the second shock for troponin I levels. Histologic sections were taken near the RV coil. Results: RAMP shocks succeeded in 6 successive pigs, while BTE shocks failed in 8 pigs before 6 successes were achieved (Fig C). Troponin I after 6 hours was ~4 times greater with BTE than with RAMP shocks (Fig 4, P Conclusions: Thus, RAMP shocks defibrillate more reliably at a lower energy and cause markedly less damage as indicated by troponin I, ST elevation, and histopathology.
Preliminary optical design of AIRES: an airborne infrared echelle spectrometer for SOFIA (1998)

NASA's Stratospheric Observatory for IR Astronomy (SOFIA) will enable unprecedented IR acuity at wavelengths obscured from the ground. To help open this new chapter in the exploration of the IR universe, we are developing the Airborne IR Echelle Spectrometer (AIRES) as a facility science instrument. Full funding was awarded for a four year development in October, 1997. The instrument is scheduled to come on-line with the observatory in the Fall of 2001. It will be used to investigate a broad range of phenomena that occur in the interstellar medium. AIRES will use a 1200 mm long, 76 degree blaze angle echelle to combine high resolution spectroscopy with diffraction-limited imaging in the cross-dispersion direction. Its three 2D detector arrays will prove good sensitivity over a decade in wavelength. An additional array will be used as a slit viewer for (lambda) &lt;EQ 28 micrometers to image source morphology and to verify telescope pointing. Our scientific motivation, preliminary optical design and packaging, focal plane configuration, echelle prototyping, and cryostat layout are described.
Supplementary Figure 1 from Distinct Effects of Ligand-Induced PDGFRα and PDGFRβ Signaling in the Human Rhabdomyosarcoma Tumor Cell and Stroma Cell Compartments (2023)

&lt;p&gt;PDF file - 882K, Antibodies directed against human full-length PDGF-CC and PDGF-DD recognize their target proteins. Antibody specificity was evaluated in paraffin-embedded COS-1 cell pellets after transient transfection with plasmid constructs expressing PDGF proteins as indicated. The transfection efficiency was estimated to be approximately 40%. Additional analyses indicated that the PDGF-CC antibody displayed some crossreactivity with the processed form of PDGF-DD (data not shown).&lt;/p&gt;
The Application of Microreactor Technology for the Synthesis of 1,2-Azoles (2003)

We demonstrate the successful synthesis of an array of 1,2-azoles within a borosilicate glass microreactor whereby conversions in the range of 98−100% were obtained. In terms of large-scale production, this corresponds to 0.339 g day-1 per microreactor when employing reagent concentrations of 1.0 M.
Matrix product quantization for very-low-rate speech coding (2002)

Efficient block coding methods for LPC information play an essential role in very-low-rate speech coding systems. The subject of this contribution is a new suboptimal matrix quantization scheme for LPC parameters, called matrix product quantization (MPQ), which operates at bit rates between 300 and 700 b/s. MPQ encodes sequences of LPC parameter vectors using a product formulation of two matrices which describe the average parameter vector and the temporal contour. In fixed-rate coding systems for mobile communication, MPQ achieves a very high coding efficiency at a low coding delay. Compared to the multi-frame coding method (MFC) of Kemp et al. (1991), which causes a delay of 8 frames, the MPQ scheme operates more efficiently even at a coding delay of only 3 frames. Applying MPQ to a variable-rate segment vocoder, a bit rate reduction of 50% compared to memoryless VQ is obtained at a frame period of 20 ms.
MODELAGEM ESTOCÁSTICA DA EVOLUÇÃO TEMPORAL DA MICROESTRUTURA DE SISTEMAS POLIMÉRICOS (2015)

RESUMO -O conhecimento da microestrutura de um material polimrico de grande interesse industrial, pois dela derivam todas as propriedades macroscpicas que definem a aplicabilidade do material. Neste trabalho, sistemas de copolimerizao so modelados estocasticamente pela utilizao do algoritmo de Gillespie, para determinao da evoluo temporal da microestrutura dos produtos copolimricos com arquitetura linear de cadeia. Tcnicas para extenso do algoritmo de Gillespie para simulao dos processos realizados em reatores contnuos so apresentadas. So modelados os processos em reatores do tipo tanque agitado sob efeito de mxima micromistura, atravs do acoplamento de um mdulo determinstico simulao estocstica,
Biochemical parameters of gluten in relation to fundamental rheological properties. (1996)

Dit IOP Industriele Eiwitten project is gericht op het leggen van relaties tussen biochemische karakteristieken van tarwegluten en hun reologisch gedrag. Op deze wijze wordt inzicht verkregen in structuurfunctie relaties van tarwegluten, met name gericht op bakkerijtoepassingen. Voor dit onderzoek zijn zes Europese tarwe-varieteiten geselecteerd, die vergelijkbare eiwitgehaltes hebben, maar een groot verschil in bakkwaliteit vertonen. Kwaliteitsverschillen tussen deze varieteiten uiten zich het meest in de benodigde kneedtijd om optimale bakkwaliteit te bereiken. Er zijn duidelijke aanwijzingen dat hieraan een aantal biochemische factoren ten grondslag liggen zoals de hoeveelheid van het glutenine polymeer, de polymerisatie ervan en de mate van fysische interacties tussen de gluten-eiwitten
Linewidth and frequency jitter measurement of an erbium-doped fiber ring laser by using a loss-compensated, delayed self-heterodyne interferometer (1992)

The single-mode linewidth of an erbium-doped, single-frequency, fiber ring laser has been measured by using a newly developed loss-compensated delayed self-heterodyne interferometer that has a resolution of less than 600 Hz. The natural linewidth is determined to have an upper bound of less than 2 kHz. In addition, frequency jitter was found to be dominant over the natural mately 4 kHz.
Aspartate-β-Hydroxylase: A Promising Target to Limit the Local Invasiveness of Colorectal Cancer (2020)

Colorectal cancer's (CRC) ability to invade local tissues and lymph nodes and generate distant metastases is the key for TNM classification. Aspartate-β-hydroxylase (ASPH), a transmembrane protein that catalyzes Notch receptors and ligand activation, is involved in tumor invasion. Because Notch is involved in gut homeostasis, it could be a target for CRC therapy. ASPH mRNA and protein expression, promoter methylation and gene copy numbers were evaluated using the TCGA and CPTAC human CRC datasets. Using digital pathology, ASPH was scored in the luminal area (LM), center tumor (CT) and invasive margin (IM) of 100 human CRCs. The effect of ASPH targeting on invasiveness and viability was tested by siRNA knockdown and small molecule inhibitors (SMI). Bioinformatics analysis showed increased expression of ASPH mRNA and protein in CRC, paired with a decreased methylation profile. ASPH genetic gain or amplification was frequent (56%), while deletion was rare (0.03%). Digital pathology analysis showed that ASPH exerted its pathological activity in the invasive margin of the tumor, affecting invasive front morphology, tumor budding and patients' overall survival. In vitro, ASPH targeting by siRNA or SMI reduced cell invasion and growth and caused Notch-1 downregulation. This study demonstrates that ASPH targeting by specific inhibitors could improve CRC treatment strategies.
Membrane réactive pour la production électrochimique de radicaux oxydants visant le traitement de polluants bioréfractaires. (2017)

International audience
Effects of Seed Coating and Osmotic Priming on the Germination of Lettuce Seeds (1987)

Abstract Lettuce ( Lactuca sativa L. ‘Empire’) seeds (achenes) were primed in aerated solutions of polyethylene glycol 8000 (−1.56 MPa) for 24 hr at 18°C and dried to 6% water content. Control (untreated) and primed seeds then were commercially coated in a clay-based material (Royal Sluis Splitkote). Priming prevented the occurrence of thermodormancy in seeds imbibed at temperatures between 32° and 37° and accelerated the rate of germination at all temperatures tested. Coating delayed germination slightly and reduced the maximum germination temperature of control seeds. Primed seeds exhibited thermodormancy when tested immediately after coating, but they soon regained their capacity for high-temperature germination. When tested after 5 months of storage at 5°, primed seeds germinated at temperatures up to 37° regardless of coating. Osmotically primed seeds retained the capacity for germination at high temperature after seed coating and storage, providing an effective means of improving stand establishment of direct-seeded lettuce in hot weather.
Diamagnetic Response of Metallic Photonic Crystals at Infrared and Visible Frequencies (2006)

We show analytically and numerically that diamagnetic response (effective magnetic permeability mue<1) at infrared and visible frequencies can be achieved in photonic crystals composed of metallic nanowires or nanospheres when the wavelength is much larger than the lattice constant a (lambda approximately 2000a). When lambda approximately100a, the metallic photonic crystals will exhibit strong diamagnetic response (mue<0.8), leading to many interesting phenomena such as the unusual Brewster angle for s waves and incident-angle-and-polarization-independent reflection and transmission.
Induction of a transmissible tau pathology by traumatic brain injury (2018)

Traumatic brain injury is a risk factor for subsequent neurodegenerative disease, including chronic traumatic encephalopathy, a tauopathy mostly associated with repetitive concussion and blast, but not well recognized as a consequence of severe traumatic brain injury. Here we show that a single severe brain trauma is associated with the emergence of widespread hyperphosphorylated tau pathology in a proportion of humans surviving late after injury. In parallel experimental studies, in a model of severe traumatic brain injury in wild-type mice, we found progressive and widespread tau pathology, replicating the findings in humans. Brain homogenates from these mice, when inoculated into the hippocampus and overlying cerebral cortex of naïve mice, induced widespread tau pathology, synaptic loss, and persistent memory deficits. These data provide evidence that experimental brain trauma induces a self-propagating tau pathology, which can be transmitted between mice, and call for future studies aimed at investigating the potential transmissibility of trauma associated tau pathology in humans.
A novel sensorless support vector regression based multi-stage algorithm to track the maximum power point for photovoltaic systems (2013)

This paper proposes a new approach for maximum power-point tracking (MPPT) process in photovoltaic (PV) systems. Based on the theory of support vector regression (SVR), a multi-stage algorithm (MSA) is proposed for MPPT to estimate the temperature and solar irradiation without a need to measure them. The only needed measurements for the proposed MSA are the output voltage and current of the PV panel. The MSA consists of three stages: The first stage estimates the initial values of temperature and irradiation; the second stage instantaneously estimates the irradiation assuming that the temperature is constant within a one-hour time span; and the third stage updates the estimated temperature once every one hour. The proposed method is robust, not only to changes in solar irradiation and load, but also to variations in temperature. Moreover, using fewer sensors improves the reliability of the system. The effectiveness of the proposed method is demonstrated through simulation studies conducted in the PSCAD/EMTDC and Matlab software environment.
Thirty-Day Outcomes of Transcatheter Mitral Valve Replacement for Degenerated Mitral Bioprostheses (Valve-in-Valve), Failed Surgical Rings (Valve-in-Ring), and Native Valve With Severe Mitral Annular Calcification (Valve-in-Mitral Annular Calcification) in the United States (2020)

MViV using aortic balloon-expandable transcatheter heart valves is associated with a low complication rate, a 30-day mortality lower than predicted by the Society of Thoracic Surgeons score, and superior short-term outcomes than MViR and ViMAC. At 30 days, patients in all groups experienced improvement of symptoms, and valve performance remained stable. Registration: URL: https://www.clinicaltrials.gov; Unique identifier: NCT02245763.
Evaluation of vehicle-level MIMO antennas: Capacity, total embedded efficiency, and envelope correlation (2014)

The paper describes an approach to estimate 2×2 MIMO channel capacity of a shark-fin radome antenna designed for multiple LTE bands by using channel matrices measured from drive tests in a real driving environment with a commercially available LTE radio network analyzer. The nonproduction shark-fin radome antenna that is mounted on the roof of a sedan is treated as a black box, and the estimated channel capacity is compared with that of a reference MIMO antenna consisting of identical monopoles tuned at 750 MHz. The two element λ/4 monopole array was also measured in a rich isotropic multipath environment at a reverberation chamber so that it can be used as a reference with known channel capacity. Total embedded efficiency and envelope correlation derived from measured s-parameters for the black box and the reference antennas are presented and discussed. The results show that the channel capacity depends on both the embedded efficiency and the correlation, and the approach estimating the MIMO throughput based on the channel matrices measured from the drive tests is shown to be useful to evaluate multiple MIMO antennas in a real driving environment.
Viral Packaging and Cell Culture for CRISPR-Based Screens (2016)

This protocol describes how to perform the tissue culture and high-throughput sequencing library preparation for a CRISPR-based screen. First, pantropic lentivirus is prepared from a sgRNA plasmid pool and applied to the target cells. Following antibiotic selection and a harvest of the initial population, cells are then cultured under the desired screening condition(s) for 14 population doublings. sgRNA barcode sequences integrated in the genomic DNA of each cell population are amplified and subject to high-throughput sequencing. Guidelines for downstream analysis of the sequencing data are also provided.
Shape-dependent sensitivity of single plasmonic nanoparticles for biosensing (2009)

Shape-dependent sensitivity of localized surface plasmon-based biosensing was investigated by combining single-particle protein-sensing and multiple multipole program simulation. Significantly higher sensitivity was observed for tetrahedral particles than spherical ones, which was revealed by careful structural analysis of individually measured particles. The simulation of the corresponding particles with layered protein adsorption model showed consistent optical property and sensitivity, which were explained in terms of the field enhancement at the pointing edges.
Giant T-wave inversion in an implantable cardioverter-defibrillator patient with ischaemic cardiomyopathy: what is the mechanism? (2021)

Giant T-wave inversion in an implantable cardioverter-defibrillator patient with ischaemic cardiomyopathy: what is the mechanism? Hussam Ali, Hussam Ali Arrhythmia & Electrophysiology Center, IRCCS—MultiMedica Group, Via Milanese 300, 20099, Sesto San Giovanni, Milan, Italy Corresponding author. Tel: +39 02 24209400. E-mail address: hussamali.ep@gmail.com https://orcid.org/0000-0002-3469-1028 Search for other works by this author on: Oxford Academic PubMed Google Scholar Pierpaolo Lupo, Pierpaolo Lupo Arrhythmia & Electrophysiology Center, IRCCS—MultiMedica Group, Via Milanese 300, 20099, Sesto San Giovanni, Milan, Italy Search for other works by this author on: Oxford Academic PubMed Google Scholar Guido De Ambroggi, Guido De Ambroggi Arrhythmia & Electrophysiology Center, IRCCS—MultiMedica Group, Via Milanese 300, 20099, Sesto San Giovanni, Milan, Italy Search for other works by this author on: Oxford Academic PubMed Google Scholar Sara Foresti, Sara Foresti Arrhythmia & Electrophysiology Center, IRCCS—MultiMedica Group, Via Milanese 300, 20099, Sesto San Giovanni, Milan, Italy Search for other works by this author on: Oxford Academic PubMed Google Scholar Riccardo Cappato Riccardo Cappato Arrhythmia & Electrophysiology Center, IRCCS—MultiMedica Group, Via Milanese 300, 20099, Sesto San Giovanni, Milan, Italy Search for other works by this author on: Oxford Academic PubMed Google Scholar EP Europace, euab101, https://doi.org/10.1093/europace/euab101 Published: 03 June 2021 Article history Received: 14 February 2021 Editorial decision: 26 March 2021 Accepted: 30 March 2021 Published: 03 June 2021