gebhart/patrepeval huggingface dataset:
Ca2+ Entry Induced by Cyclic ADP-ribose in Intact T-Lymphocytes (1997)

Cyclic ADP-ribose (cADPr) is a potent Ca2+-mobilizing natural compound (Lee, H. C., Walseth, T. F., Bratt, G. T., Hayes, R. N., and Clapper, D. L. (1989) J. Biol. Chem. 264, 1608-1615) which has been shown to release Ca2+ from an intracellular store of permeabilized T-lymphocytes (Guse, A. H., Silva, C. P., Emmrich, F., Ashamu, G., Potter, B. V. L., and Mayr, G. W. (1995) J. Immunol. 155, 3353-3359). Microinjection of cADPr into intact single T lymphocytes dose dependently induced repetitive but irregular Ca2+ spikes which were almost completely dependent on the presence of extracellular Ca2+. The Ca2+ spikes induced by cADPr could be blocked either by co-injection of cADPr with the specific antagonist 8-NH2-cADPr, by omission of Ca2+ from the medium, or by superfusion of the cells with Zn2+ or SK-F 96365. Ratiometric digital Ca2+ imaging revealed that single Ca2+ spikes were initiated at several sites ("hot spots") close to the plasma membrane. These hot spots then rapidly formed a circular zone of high Ca2+ concentration below the plasma membrane which subsequently propagated like a closing optical diaphragm into the center of the cell. Taken together these data indicate a role for cADPr in Ca2+ entry in T-lymphocytes.
Haploinsufficiency of immune checkpoint receptor CTLA4 induces a distinct neuroinflammatory disorder (2020)

BACKGROUNDCytotoxic T lymphocyte antigen 4 (CTLA4) is essential for immune homeostasis. Genetic mutations causing haploinsufficiency (CTLA4h) lead to a phenotypically heterogenous, immune-mediated disease that can include neuroinflammation. The neurological manifestations of CTLA4h are poorly characterized.METHODSWe performed an observational natural history study of 50 patients with CTLA4h who were followed at the NIH. We analyzed clinical, radiological, immunological, and histopathological data.RESULTSEvidence for neuroinflammation was observed in 32% (n = 16 of 50) of patients in this cohort by magnetic resonance imaging (MRI) and/or by cerebrospinal fluid analysis. Clinical symptoms were commonly absent or mild in severity, with headaches as the leading complaint (n = 13 of 16). The most striking findings were relapsing, large, contrast-enhancing focal lesions in the brain and spinal cord observed on MRI. We detected inflammation in the cerebrospinal fluid and leptomeninges before the parenchyma. Brain biopsies of inflammatory lesions from 10 patients showed perivascular and intraparenchymal mixed cellular infiltrates with little accompanying demyelination or neuronal injury.CONCLUSIONSNeuroinflammation due to CTLA4h is mediated primarily by an infiltrative process with a distinct and striking dissociation between clinical symptoms and radiological findings in the majority of patients.FUNDINGNIAID, NIH, Division of Intramural Research, NINDS, NIH, Division of Intramural Research, and the National Multiple Sclerosis Society-American Brain Foundation.TRIAL REGISTRATIONClinicalTrials.gov NCT00001355.
Inhibition of IL-17 and IL-23 in Human Keratinocytes by the A3 Adenosine Receptor Agonist Piclidenoson (2018)

Interleukin-17 and interleukin-23 play major roles in the inflammatory process in psoriasis. The Gi protein-associated A<sub>3</sub> adenosine receptor (A<sub>3</sub>AR) is known to be overexpressed in inflammatory cells and in peripheral blood mononuclear cells (PBMCs) of patients with autoimmune inflammatory conditions. Piclidenoson, a selective agonist at the A<sub>3</sub>AR, induces robust anti-inflammatory effect in psoriasis patients. In this study, we aimed to explore A<sub>3</sub>AR expression levels in psoriasis patients and its role in mediating the anti-inflammatory effect of piclidenoson in human keratinocyte cells. A<sub>3</sub>AR expression levels were evaluated in skin tissue and PBMCs derived from psoriasis patients and healthy subjects. Proliferation assay and the expression of signaling proteins were used to evaluate piclidenoson effect on human keratinocytes (HaCat). High A<sub>3</sub>AR expression levels were found in a skin biopsy and in PBMCs from psoriasis patients in comparison to healthy subjects. Piclidenoson inhibited the proliferation of HaCat cells through deregulation of the NF-<i>κ</i>B signaling pathway, leading to a decrease in interleukin-17 and interleukin-23 expression levels. This effect was counteracted by the specific antagonist MRS 1523. A<sub>3</sub>AR overexpression in skin and PBMCs of psoriasis patients may be used as a target to inhibit pathological cell proliferation and the production of interleukin-17 and interleukin-23.
Comparative Study of Foreign Object and Misalignment in Inductive Power Transfer Systems (2019)

This paper aims to identify the difference between foreign object (FO) and misalignment in terms of their influence on inductive power transfer (IPT) systems. This is performed through magnetic and equivalent circuit analysis of the mutual inductance, primary input impedance, charging pad terminal impedance and current harmonics. Experiment measurements on an IPT prototype are carried out to verify the analysis. It is found that: the charging pad terminal impedance under FO condition has a more pronounced decrement than that of misalignment; the mutual inductance under FO condition shows negative correlation with frequency, while positive for misalignment; the absolute value of the input impedance is decreased by FO and increased by misalignment; the influence of FO and misalignment on the THD of the input current is minimal. Finally, it is possible to detect FO and distinguish it from misalignment, through the variation of the primary pad terminal inductance, as well as the frequency dependence of the primary input impedance and the mutual inductance.
Controlling Long-Range Genomic Interactions to Reprogram the β-Globin Locus (2012)

Abstract Abstract 280 Distal enhancers physically contact target promoters to confer high level transcription. At the mammalian β-globin loci long-range chromosomal interactions between a distal enhancer, called the locus control region (LCR), and the globin genes are developmentally dynamic such that the LCR contacts the embryonic, fetal and adult globin genes in a stage-appropriate fashion. LCR-globin gene interactions require the nuclear factor Ldb1. Recently, we employed artificial zinc finger (ZF) proteins to target Ldb1 to the endogenous β-globin locus to force an LCR-promoter interaction. This led to substantial activation of β-globin transcription and suggested that forced chromatin looping could be employed as a powerful tool to manipulate gene expression in vivo (Deng et al., Cell 2012). Reactivation of the fetal globin genes in adult erythroid cells has been a long-standing goal in the treatment of patients with sickle cell anemia. Therefore, building on our findings, we investigated whether the developmentally silenced embryonic globin gene βh1 can be re-activated in adult murine erythroblasts by re-directing the LCR away from the adult type globin gene and towards its embryonic counterpart. To this end, Ldb1 was fused to artificial ZF proteins (ZF-Ldb1) designed to bind to the βh1 promoter. ZF-Ldb1 was introduced into definitive erythroid cells in which only the adult but not the embryonic β-like globin gene is expressed. In vivo binding of the ZF-Ldb1 to its intended target was verified by chromatin immunoprecipitation assay. Strikingly, expression of ZF-Ldb1 re-activated βh1 transcription up to approximately ∼15% of total cellular β-globin production. This suggests that forced tethering of a looping factor to a select promoter can be employed to override a pre-existing developmental long-range chromatin interaction to reprogram a developmentally controlled gene locus. We are now in the process of testing whether our approach might be suitable to reactivate the silent fetal globin genes in adult human erythroid cells. These studies are underway and the results will be discussed at the meeting. Disclosures: Reik: Sangamo BioSciences, Inc.: Employment. Gregory:Sangamo BioSciences, Inc.: Employment.
Supplementary Figures 1 - 2 from mTOR Inhibition Improves Antitumor Effects of Vaccination with Antigen-Encoding RNA (2023)

&lt;p&gt;PDF file - 1926K, Description of effects of Rapamycin on CD8 T cell phenotype.&lt;/p&gt;
Digital frequency offset compensation in high-speed optical intersatellite data transmission systems (2015)

We present an alternative approach for current homodyne detection systems in optical intersatellite links. Our approach is based on digital frequency offset compensation instead of optical phase-locked loop techniques, in order to achieve a more flexible coherent detection scheme.
Solid-State NMR Investigation of the Selective Disruption of Lipid Membranes by Protegrin-1 (2004)

The interaction of a beta-hairpin antimicrobial peptide, protegrin-1 (PG-1), with various lipid membranes is investigated by (31)P, (2)H, and (13)C solid-state NMR. Mixed lipid bilayers containing anionic lipids and cholesterol are used to mimic the bacterial and mammalian cell membranes, respectively. (31)P and (2)H spectra of macroscopically oriented samples show that PG-1 induces the formation of an isotropic phase in anionic bilayers containing phosphatidylglycerol. Two-dimensional (31)P exchange experiments indicate that these isotropic lipids are significantly separate from the residual oriented lamellar bilayers, ruling out toroidal pores as the cause for the isotropic signal. (1)H spin diffusion experiments show that PG-1 is not exclusively bound to the isotropic phase but is also present in the residual oriented lamellar bilayers. This dynamic and morphological heterogeneity of the anionic membranes induced by PG-1 is supported by the fact that (13)C T(2) relaxation times measured under cross polarization and direct polarization conditions differ significantly. In contrast to the anionic membrane, the zwitterionic phosphatidylcholine (PC) membrane does not form an isotropic phase in the presence of PG-1 but shows significant orientational disorder. The addition of cholesterol to the PC bilayer significantly reduces this orientational disorder. The (13)C T(2) relaxation times of the PC lipids in the presence of both cholesterol and PG-1 suggest that the peptide may decrease the dynamic heterogeneity of the cholesterol-containing membrane. The observed selective interaction of PG-1 with different lipid membranes is consistent with its biological function and may be caused by its strong cationic and amphipathic structure.
A one-dimensional polymeric ribbon alternative to the aggregation of d8 ML4 fragments (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA one-dimensional polymeric ribbon alternative to the aggregation of d8 ML4 fragmentsRoald Hoffmann, Christian Minot, and Harry B. GrayCite this: J. Am. Chem. Soc. 1984, 106, 7, 2001–2005Publication Date (Print):April 1, 1984Publication History Published online1 May 2002Published inissue 1 April 1984https://pubs.acs.org/doi/10.1021/ja00319a019https://doi.org/10.1021/ja00319a019research-articleACS PublicationsRequest reuse permissionsArticle Views88Altmetric-Citations29LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Participation of the Salmonella OmpD Porin in the Infection of RAW264.7 Macrophages and BALB/c Mice (2014)

Salmonella Typhimurium is the etiological agent of gastroenteritis in humans and enteric fever in mice. Inside these hosts, Salmonella must overcome hostile conditions to develop a successful infection, a process in which the levels of porins may be critical. Herein, the role of the Salmonella Typhimurium porin OmpD in the infection process was assessed for adherence, invasion and proliferation in RAW264.7 mouse macrophages and in BALB/c mice. In cultured macrophages, a ΔompD strain exhibited increased invasion and proliferation phenotypes as compared to its parental strain. In contrast, overexpression of ompD caused a reduction in bacterial proliferation but did not affect adherence or invasion. In the murine model, the ΔompD strain showed increased ability to survive and replicate in target organs of infection. The ompD transcript levels showed a down-regulation when Salmonella resided within cultured macrophages and when it colonized target organs in infected mice. Additionally, cultured macrophages infected with the ΔompD strain produced lower levels of reactive oxygen species, suggesting that down-regulation of ompD could favor replication of Salmonella inside macrophages and the subsequent systemic dissemination, by limiting the reactive oxygen species response of the host.
Pyrazolo[1,5-a]pyridine Inhibitor of the Respiratory Cytochrome bcc Complex for the Treatment of Drug-Resistant Tuberculosis (2018)

Respiration is a promising target for the development of new antimycobacterial agents, with a growing number of compounds in clinical development entering this target space. However, more candidate inhibitors are needed to expand the therapeutic options available for drug-resistant Mycobacterium tuberculosis infection. Here, we characterize a putative respiratory complex III (QcrB) inhibitor, TB47: a pyrazolo[1,5- a]pyridine-3-carboxamide. TB47 is active (MIC between 0.016 and 0.500 μg/mL) against a panel of 56 M. tuberculosis clinical isolates, including 37 multi-drug-resistant and two extensively drug-resistant strains. Pharmacokinetic and toxicity studies showed promising profiles, including negligible CYP450 interactions, cytotoxicity, and hERG channel inhibition. Consistent with other reported QcrB inhibitors, TB47 inhibits oxygen consumption only when the alternative oxidase, cytochrome bd, is deleted. A point mutation in the qcrB cd2-loop (H190Y, M. smegmatis numbering) rescues the inhibitory effects of TB47. Metabolomic profiling of TB47-treated M. tuberculosis H37Rv cultures revealed accumulation of steps in the TCA cycle and pentose phosphate pathway that are linked to reducing equivalents, suggesting that TB47 causes metabolic redox stress. In mouse infection models, a TB47 monotherapy was not bactericidal. However, TB47 was strongly synergistic with pyrazinamide and rifampicin, suggesting a promising role in combination therapies. We propose that TB47 is an effective lead compound for the development of novel tuberculosis chemotherapies.
Multi-Target Engagement in Complex Mobile Surveillance Sensor Networks (2017)

Efficient use of the network’s resources to collect information about objects (events) in a given volume of interest (VOI) is a key challenge in large-scale sensor networks. Multi-sensor multi-target tracking in surveillance applications is an example where the network’s success in tracking targets, efficiently and effectively, hinges significantly on the network’s ability to allocate the right set of sensors to the right set of targets so as to achieve optimal performance which minimizes the number of uncovered targets. This task can be even more complicated when both the sensors and the targets are mobile. To ensure timely tracking of mobile targets, the surveillance sensor network needs to perform the following tasks in real-time: (i) target-to-sensor allocation; (ii) sensor mobility control and coordination. The computational complexity of these two tasks presents a challenge, particularly in large scale dynamic network applications. This paper proposes a formulation based on the Semi-flocking algorithm and the distributed constraint optimization problem (DCOP). The semi-flocking algorithm performs multi-target motion control and coordination, a DCOP modeling algorithm performs the target engagement task. As will be demonstrated experimentally in the paper, this algorithmic combination provides an effective approach to the multi-sensor/multi-target engagement problem, delivering optimal target coverage as well as maximum sensors utilization.
Text simplification for reading assistance (2003)

This paper describes our ongoing research project on text simplification for congenitally deaf people. Text simplification we are aiming at is the task of offering a deaf reader a syntactic and lexical paraphrase of a given text for assisting her/him to understand what it means. In this paper, we discuss the issues we should address to realize text simplification and report on the present results in three different aspects of this task: readability assessment, paraphrase representation and post-transfer error detection.
Poly(n-butyl acrylate) Homopolymer and Poly[n-butyl acrylate-b-(n-butyl acrylate-co-styrene)] Block Copolymer Prepared via Nitroxide-Mediated Living/Controlled Radical Polymerization in Miniemulsion (2001)

ADVERTISEMENT RETURN TO ISSUEPREVCommunication to the...Communication to the EditorNEXTPoly(n-butyl acrylate) Homopolymer and Poly[n-butyl acrylate-b-(n-butyl acrylate-co-styrene)] Block Copolymer Prepared via Nitroxide-Mediated Living/Controlled Radical Polymerization in MiniemulsionCéline Farcet, Bernadette Charleux, and Rosangela PirriView Author Information Laboratoire de Chimie Macromoléculaire, Unité Mixte de Recherche associée au CNRS (UMR 7610), Université Pierre et Marie Curie, Tour 44, 1er étage, 4, place Jussieu, 75252 Paris, Cedex 05, France, and ATOFINA, Groupement de Recherches de Lacq, B.P. no. 34, 64170 LACQ, France Cite this: Macromolecules 2001, 34, 12, 3823–3826Publication Date (Web):May 5, 2001Publication History Received2 January 2001Published online5 May 2001Published inissue 1 June 2001https://pubs.acs.org/doi/10.1021/ma010011shttps://doi.org/10.1021/ma010011srapid-communicationACS PublicationsCopyright © 2001 American Chemical SocietyRequest reuse permissionsArticle Views1920Altmetric-Citations75LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Emulsions,Monomers,Organic compounds,Radical polymerization,Styrenes Get e-Alerts
Interface and Electrode Engineering for Next-Generation Organic Photovoltaic Cells: Final Technical Report, March 2005 - August 2008 (2008)

The objective of this project was to enable next-generation, efficient, easily manufacturable, and durable organic photovoltaics through interface and electrode engineering.
Thermal via allocation for 3D ICs considering temporally and spatially variant thermal power (2006)

All existing methods for thermal-via allocation are based on a steady-state thermal analysis and may lead to excessive number of thermal vias. This paper develops an accurate and efficient thermal-via allocation considering temporally and spatially variant thermal-power. The transient temperature is calculated using macromodel by a structured and parameterized model reduction, which generates temperature sensitivity with respect to thermal-via density. By defining a thermal-violation integral based on the transient temperature, a nonlinear optimization problem is formulated to allocate thermal-vias and minimize thermal violation integral. This optimization problem is transformed into a sequence of subproblems by Lagrangian relaxation, and each subproblem is solved by quadratic programming using sensitives from the macromodel. Experiments show that compared to the existing method using steady-state thermal analysis, our method is 126X faster to obtain the temperature profile, and reduces the number of thermal vias by 2.04X under the same temperature bound.
Pipeline Pumping and Compression System: A Practical Approach, Third Edition (2018)

This comprehensive professional reference has been substantially revised and updated for the third edition. It is both a training tool and reference text covering all aspects of pipeline pumping and compression system design, configuration, and operation, in addition to the dynamic behavior of all the elements in each system. The authors provide practical solutions for achieving reliable operation of these systems. This book will serve as a useful guide for the design of liquid and gas pipeline transmissions, as well as a guide to various installation options. It will be invaluable for practicing engineers in the pipeline and oil &amp; gas industry, especially those involved with the design and operation of pumping and compression systems
988: Re-Evaluation of the TNM Staging of Conventional Renal Cortical Tumors: Outcome of T1N0M0 and T3AN0M0 Renal Cortical Tumors are Equivalent (2005)

You have accessJournal of UrologyDiscussed Poster, Tuesday, May 24, 2005, 8:00 am - 12:00 pm1 Apr 2005988: Re-Evaluation of the TNM Staging of Conventional Renal Cortical Tumors: Outcome of T1N0M0 and T3AN0M0 Renal Cortical Tumors are Equivalent Alana M. Murphy, Scott M. Gilbert, Eric T. Goluboff, Aaron E. Katz, Carl A. Olsson, Thor S. Sawczuk, Mitchell C. Benson, and James M. McKiernan Alana M. MurphyAlana M. Murphy More articles by this author , Scott M. GilbertScott M. Gilbert More articles by this author , Eric T. GoluboffEric T. Goluboff More articles by this author , Aaron E. KatzAaron E. Katz More articles by this author , Carl A. OlssonCarl A. Olsson More articles by this author , Thor S. SawczukThor S. Sawczuk More articles by this author , Mitchell C. BensonMitchell C. Benson More articles by this author , and James M. McKiernanJames M. McKiernan More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(18)35144-9AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "988: Re-Evaluation of the TNM Staging of Conventional Renal Cortical Tumors: Outcome of T1N0M0 and T3AN0M0 Renal Cortical Tumors are Equivalent." The Journal of Urology, 173(4S), p. 267 © 2016 by American Urological AssociationFiguresReferencesRelatedDetails Volume 173Issue 4SApril 2005Page: 267 Advertisement Copyright & Permissions© 2016 by American Urological AssociationMetricsAuthor Information Alana M. Murphy More articles by this author Scott M. Gilbert More articles by this author Eric T. Goluboff More articles by this author Aaron E. Katz More articles by this author Carl A. Olsson More articles by this author Thor S. Sawczuk More articles by this author Mitchell C. Benson More articles by this author James M. McKiernan More articles by this author Expand All Advertisement PDF downloadLoading ...