gebhart/patrepeval huggingface dataset:
Studies of RNA-protein interactions in the yeast 5 S ribonucleoprotein particles by fluorescence and tritium exchange. Implications for ribosomal assembly. (1988)

Studies of the conformational properties of the yeast 5 S RNA-protein complex were initiated in an attempt to understand loss of ability of its individual protein and RNA components to reassociate. The 5 S RNA-L1a protein complex from 60 S ribosomal subunits of Saccharomyces cerevisiae could be dissociated by high concentrations of magnesium. The degree of dissociation could be monitored by polyacrylamide gel electrophoresis. The complex was completely dissociated at about 390 mM magnesium, but was stable at 4 degrees C in 25 mM EDTA up to 48 h. The overall conformation of the complex was monitored using tritium exchange. The tritium exchange behavior was dramatically changed as the complex was dissociated. To determine contribution of each component to the observed overall change reflected in the tritium exchange behavior, ethidium bromide (EtBr) and bis-anilinonaphthalene-sulfonic acid fluorescence were used to monitor the RNA and the protein moiety, respectively. Upon dissociation of the complex, the fluorescence intensity resulting from EtBr binding to RNA decreased, whereas the intensity due to bis-anilinonaphthalene-sulfonic acid binding to the protein increased. Turbidity was observed during dissociation of the complex. These results indicate that disruption of interactions between the 5 S RNA and protein L1a resulted in an exposure of solvent-accessible apolar regions in the protein molecule. Such exposure led to insolubility of protein and irreversibility in interaction between individual components. Properties of the separated components also suggest that special conditions may be required for these components to associate during ribosomal assembly.
Structure−Activity Relationships in Gold Nanoparticle Dimers and Trimers for Surface-Enhanced Raman Spectroscopy (2010)

Understanding the detailed relationship between nanoparticle structure and activity remains a significant challenge for the field of surface-enhanced Raman spectroscopy. To this end, the structural and optical properties of individual plasmonic nanoantennas comprised of Au nanoparticle assemblies that are coated with organic reporter molecules and encapsulated by a SiO(2) shell have been determined using correlated transmission electron microscopy (TEM), dark-field Rayleigh scattering microscopy, surface-enhanced Raman scattering (SERS) microscopy, and finite element method (FEM) calculations. The distribution of SERS enhancement factors (EFs) for a structurally and optically diverse set of nanoantennas is remarkably narrow. For a collection of 30 individual nanoantennas ranging from dimers to heptamers, the EFs vary by less than 2 orders of magnitude. Furthermore, the EFs for the hot-spot-containing nanoparticles are uncorrelated to aggregation state and localized surface plasmon resonance (LSPR) wavelength but are crucially dependent on the size of the interparticle gap. This study demonstrates that the creation of hot spots, where two particles are in subnanometer proximity or have coalesced to form crevices, is paramount to achieving maximum SERS enhancements.
Successful Treatment of T Cell-Mediated Acute Rejection with Delayed CTLA4-Ig in Mice (2017)

Clinical observations that kidney transplant recipients receiving belatacept who experienced T cell-mediated acute rejection can be successfully treated and subsequently maintained on belatacept-based immunosuppression suggest that belatacept is able to control memory T cells. We recently reported that treatment with CTLA4-Ig from day 6 posttransplantation successfully rescues allografts from acute rejection in a BALB/c to C57BL/6 heart transplant model, in part, by abolishing B cell germinal centers and reducing alloantibody titers. Here, we show that CTLA4-Ig is additionally able to inhibit established T cell responses independently of B cells. CTLA4-Ig inhibited the <i>in vivo</i> cytolytic activity of donor-specific CD8<sup>+</sup> T cells, and the production of IFNγ by graft-infiltrating T cells. Delayed CTLA4-Ig treatment did not reduce the numbers of graft-infiltrating T cells nor prevented the accumulation of antigen-experienced donor-specific memory T cells in the spleen. Nevertheless, delayed CTLA4-Ig treatment successfully maintained long-term graft acceptance in the majority of recipients that had experienced a rejection crisis, and enabled the acceptance of secondary BALB/c heart grafts transplanted 30 days after the first transplantation. In summary, we conclude that delayed CTLA4-Ig treatment is able to partially halt ongoing T cell-mediated acute rejection. These findings extend the functional efficacy of CTLA4-Ig therapy to effector T cells and provide an explanation for why CTLA4-Ig-based immunosuppression in the clinic successfully maintains long-term graft survival after T cell-mediated rejection.
Measurements of prompt charm production cross-sections in pp collisions at s = 5 $$ \sqrt{s}=5 $$ TeV (2017)

Production cross-sections of prompt charm mesons are measured using data from pp collisions at the LHC at a centre-of-mass energy of 5 TeV. The data sample corresponds to an integrated luminosity of 8.60 0.33 pb -1 collected by the LHCb experiment. The production cross-sections of D 0 , D + , D + s , and D * + mesons are measured in bins of charm meson transverse momentum, p T , and rapidity, y. They cover the rapidity range 2.0 < y < 4.5 and transverse momentum ranges 0 < p T < 10 GeV/c for D 0 and D + and 1 < p T < 10 GeV/c for D + s and D * + mesons. The inclusive cross-sections for the four mesons, including charge-conjugate states, within the range of 1 < p T < 8 GeV/c are determined to be (pp
2–8 GHz Range High Harmonic SAW Resonator with Grooved Electrodes in LiNbO3 (2021)

This paper reports simulation and experimental study to strongly excite high harmonic SAW using a grooved interdigital transducer (IDT). The grooved IDT is made by etching LiNbO <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</inf> (LN) and filling the grooves with metal. Simulation was done to find promising designs. Then, SAW resonators with wavelengths (λ) of 5.2 µm and 1.3 µm were fabricated on 26°YX LN using Al for IDTs. Strong excitation of high harmonic SAW was confirmed. The SAW resonator with λ of 1.3 µm generated the 3rd harmonic SAW at 8.1 GHz with an impedance ratio of 32 dB.
Overexpression of the major vault transporter protein lung-resistance protein predicts treatment outcome in acute myeloid leukemia (1996)

The monoclonal antibody LRP56 recognizes a 110-kD major vault protein (lung-resistance protein [LRP]) overexpressed in several P-glycoprotein-negative (Pgp-), multidrug resistant tumor cell lines. To determine the frequency of LRP overexpression, its prognostic significance, and its relation to Pgp, we analyzed bone marrow specimens from 87 consecutive patients with acute leukemia. Diagnoses included de novo acute myeloid leukemia (AML; 21 patients), leukemia arising from an antecedent hematologic disorder or prior cytotoxic therapy (secondary AML; 27 patients), AML in relapse (29 patients), and blast phase of chronic myeloid leukemia (CML-BP; 10 patients). A granular cytoplasmic staining pattern was detected by immunocytochemistry in 32 (37%) cases, including 7 (33%) de novo AML, 13 (48%) secondary AML, 11 (38%) relapsed AML, and 1 of 10 CML-BP. Among 66 evaluable patients with AML, LRP overexpression was associated with an inferior response to induction chemotherapy (P = .0017). Remissions were achieved in 35% of LRP+ patients as compared with 68% of LRP- patients. Although Pgp adversely affected response in univariate analysis (P = .0414), only LRP had independent prognostic significance when compared in a logistic regression model (P = .0046). Differences in remission duration (P = .075) and overall survival (P = .058) approached significance only for LRP. Sequential specimens from remitting patients receiving treatment with the Pgp modulator cyclosporin-A showed emergence of the LRP phenotype despite a decrease or loss of Pgp at the time of treatment failure (P =.0304). Significant associations were observed between LRP and age greater than 55 years (P = .017), Pgp (P = .040), and prior treatment with mitoxantrone (P = .020) but not with CD34. These findings indicate that overexpression of the novel transporter protein LRP is an important predictor of treatment outcome in AML.
Slow propagation of 2 GHz acoustical waves in a suspended GaAs on insulator phononic waveguide (2020)

Optoelectronic oscillators have dominated the scene of microwave oscillators in the last few years<br> thanks to their great performances regarding frequency stability and phase noise. However, miniaturization<br> of such a device is an up to date challenge. Recently, devices based on phonon-photon<br> interaction gather a lot of interest thanks to their extreme compactness and working frequency<br> directly in the GHz. In this frame, a still missing element to obtain long-term frequency stability<br> performances is an on-chip delay within the feedback loop. Here, we experimentally show ltering<br> and slow propagation of 2 GHz acoustic waves on a Gallium Arsenide membrane heterogeneously<br> integrated on silicon wafer. By engineering the dispersion of an acoustical waveguide, we evidence<br> a group velocity below 1000 m/s for the mode able to propagate. Thus, an integrated delay implementation<br> is at reach for potential improvement of opto-acoustic devices such as optomechanical<br> oscillators or wireless applications.
Calculated Hydride Donor Abilities of Five-Coordinate Transition Metal Hydrides [HM(diphosphine)2]+ (M = Ni, Pd, Pt) as a Function of the Bite Angle and Twist Angle of Diphosphine Ligands (2008)

Density functional theory (BLYP and B3LYP) and the polarized continuum model (PCM-UA0) for solvation have been used to investigate the effect of bite angle (P−M−P) of diphosphine ligands and the dihedral or twist angle between diphosphine ligands on the hydride donor abilities of Ni, Pd, and Pt [HM(diphosphine)2]+ complexes. It is found that an increased bite angle for a given transition metal atom results in poorer hydride donor abilities. However, hydride donor abilities for these complexes also decrease as the size of the alkyl side groups on the phosphorus atom increase (Et > Me > H) and with the length of the metal phosphorus bond (Ni > Pd ≅ Pt). These trends correlate with an increase in the twist angle between the two diphosphine ligands, which increases from 0° for a square-planar configuration to 90° for a tetrahedral geometry. Shorter M−P bonds, larger substituents on the diphosphine ligands, and larger bite angles all result in increased steric interactions between diphosphine ligands and larger dihedral or twist angles between the diphosphine ligands. The twist angle correlates much more strongly with hydride donor abilities than do bite angles alone. As the twist angle increases, the hydride donor ability decreases in a linear fashion. A frontier orbital analysis has been carried out, and it is shown that the hydride donor ability of [HM(diphosphine)2]+ complexes is largely determined by the energy of the lowest unoccupied molecular orbital of the corresponding [M(diphosphine)2]2+ complex.
One-Step Synthesis of an Adaptive Nanographene MOF: Adsorbed Gas-Dependent Geometrical Diversity (2019)

A layered metal-organic framework (MOF) comprising extra-large nanographene sheets, <sup><b>HBC</b></sup><b>MOF</b>, was successfully synthesized using a dicarboxylic acid derivative of hexa-<i>peri</i>-hexabenzocoronene (<sup>HBC</sup>LH<sub>2</sub>), and its structure was characterized by single-crystal X-ray diffraction analysis. The crystal structure shows that 2D layers composed of a dinuclear Zn<sup>2+</sup> complex unit and <sup>HBC</sup>L are located on top of each other through multiple weak interlayer bonds, affording <sup><b>HBC</b></sup><b>MOF</b>, having three dimensionally connected nanopores with large nanographene surfaces. The HBC-based nanographene sheets are anchored to the MOF framework via two zinc carboxylate linkages and therefore have an axial rotational freedom. The sorption isotherms of gaseous molecules such as carbon dioxide and hydrocarbons (acetylene, propane, propylene, benzene, and cyclohexane) on <sup><b>HBC</b></sup><b>MOF</b> all displayed a hysteretic profile with reversible structural changes, as observed by in situ powder X-ray diffraction studies.
Establishing normal metabolism and differentiation in hepatocellular carcinoma cells by culturing in adult human serum (2018)

Tissue culture medium routinely contains fetal bovine serum (FBS). Here we show that culturing human hepatoma cells in their native, adult serum (human serum, HS) results in the restoration of key morphological and metabolic features of normal liver cells. When moved to HS, these cells show differential transcription of 22-32% of the genes, stop proliferating, and assume a hepatocyte-like morphology. Metabolic analysis shows that the Warburg-like metabolic profile, typical for FBS-cultured cells, is replaced by a diverse metabolic profile consistent with in vivo hepatocytes, including the formation of large lipid and glycogen stores, increased glycogenesis, increased beta-oxidation and ketogenesis, and decreased glycolysis. Finally, organ-specific functions are restored, including xenobiotics degradation and secretion of bile, VLDL and albumin. Thus, organ-specific functions are not necessarily lost in cell cultures, but might be merely suppressed in FBS. The effect of serum is often overseen in cell culture and we provide a detailed study in the changes that occur and provide insight in some of the serum components that may play a role in the establishment of the differentiated phenotype.
Broadband terahertz antireflective microstructures on quartz crystal surface by CO2 laser micro-processing (2019)

Anti-reflection (AR) coating is a critical technology and an ongoing challenge for terahertz systems. The subwavelength structure (SWS) is an effective AR method, whereas the current manufacturing techniques, such as chemical etching and ultrafast laser processing, are low-efficient and low-quality for processing structures at the hundred-micron scale on hard brittle materials. We present a study of broadband SWSs directly ablated on the surface of quartz crystal by precisely controlled CO<sub>2</sub> laser pulses, instead of commonly used ultra-fast lasers. The processing time of SWS can be shortened by two orders of magnitude compared with that by ultra-fast laser pulses. The SWS samples exhibit excellent AR properties with maximum transmittance of 97% at 0.71 THz, peak transmittance improvement of 13.5%, and optimal efficiency spectrum of 0.28-1.21 THz with transmittance >90%. The AR properties of SWS samples are in agreement with the simulated expectation and exist over a wide range of incidence angles up to ∼40°. The imaging of an object using SWS as the substrate shows an obvious improvement in imaging quality. We present an efficient and practical way to improve the transmission of optical components of materials, such as quartz crystal, alumina, and sapphire, in the terahertz band.
ChemInform Abstract: REACTION OF TUNGSTEN(VI) ALKYLIDYNE COMPLEXES WITH ACETYLENES TO GIVE TUNGSTENACYCLOBUTADIENE AND CYCLOPENTADIENYL COMPLEXES (1983)

Abstract Das Tetrachloroalkylidinwolframat (I) reagiert mit Alkinen zu Produkten, für die aufgrund einer Röntgenstrukturanalyse des Methyl‐Derivats (RG P2 1 /c, Z=4) die Cyclopentadienylalkin‐Struktur (III) bestätigt wird.
Failure analysis of PB-1 (EBTS Be/Cu mockup) (1996)

Failure analysis was done on PB-1 (series of Be tiles joined to Cu alloy) following a tile failure during a high heat flux experiment in EBTS (electron beam test system). This heat flux load simulated ambient conditions inside ITER; the Be tiles were bonded to the Cu alloy using low-temperature diffusion bonding, which is being considered for fabricating plasma facing components in ITER. Results showed differences between the EBTS failure and a failure during a room temperature tensile test. The latter occurred at the Cu-Be interface in an intermetallic phase formed by reaction of the two metals at the bonding temperature. Fracture strengths measured by these tests were over 300 MPa. The high heat flux specimens failed at the Cu-Cu diffusion bond. Fracture morphology in both cases was a mixed mode of dimple rupture and transgranular cleavage. Several explanations for this difference in failure mechanism are suggested.
ChemInform Abstract: Discovery of Imidazo(1,2‐c)pyrimidin‐5(6H)‐one Heterosubstituted Nucleoside Analogues with Potent Activity Against Human Hepatitis B Virus in vitro. (1997)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Barium iodide and strontium iodide crystals and scintillators implementing the same (2023)

In one embodiment, a crystal includes at least one metal halide; and an activator dopant comprising ytterbium. In another general embodiment, a scintillator optic includes: at least one metal halide doped with a plurality of activators, the plurality of activators comprising: a first activator comprising europium, and a second activator comprising ytterbium. In yet another general embodiment, a method for manufacturing a crystal suitable for use in a scintillator includes mixing one or more salts with a source of at least one dopant activator comprising ytterbium; heating the mixture above a melting point of the salt(s); and cooling the heated mixture to a temperature below the melting point of the salts. Additional materials, systems, and methods are presented.
<title>New approach to laser image processing for dynamic scenes</title> (1997)

This paper presents the signal processing method of laser images used for detection of small collinear obstacles in helicopter airborne applications. It is very difficult, if not almost impossible, for a regular passive imaging sensor based on CCD/FLIR detectors to detect such small, remote objects. It was shown in the literature that the fusion of range and intensity data is needed for such a task. The paper presents a new and improved algorithm for real time image processing which enable detection of small tiny objects, from a fast moving platform. Two issues related to 3D visual sensing from laser sensors are addressed here. First, concerning the filtering and reduction of the large amount of data to be coped with for enabling real-time detection with low false alarm rate, in any ambient light conditions. Second, the fusion of range and intensity data for detecting small objects form cluttered data with high probability of detection. The preliminary experimental results show the potential of the proposed algorithm, based on spatio-temporal integration, to detect with high probability such objects like electrical cables from ranges of up to 400 meters.
Multi-institutional intervention to improve patient perception of physician empathy in emergency care (2021)

An educational intervention with the addition of text reminders designed to increase empathic behaviour was not associated with a change in patient-perceived empathy, but was associated with a modest improvement in trust in physicians.
Urolithiasis, Endourology and Laparoscopy (2008)

No AccessJournal of UrologyAdult Urology1 Jun 2008Urolithiasis, Endourology and Laparoscopyis corrected byErratum Ralph ClaymanM.D. Ralph ClaymanRalph Clayman More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2008.02.017AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "Urolithiasis, Endourology and Laparoscopy." The Journal of Urology, 179(6), pp. 2360–2361 © 2008 by American Urological AssociationFiguresReferencesRelatedDetailsRelated articlesJournal of UrologyJun 20, 2008, 12:00:00 AMErratum Volume 179Issue 6June 2008Page: 2360-2361 Advertisement Copyright & Permissions© 2008 by American Urological AssociationMetricsAuthor Information Ralph Clayman More articles by this author Expand All Advertisement PDF DownloadLoading ...
Rationale and design of the Drug‐Eluting Stents vs Bare‐Metal Stents in Saphenous Vein Graft Angioplasty (DIVA) Trial (2017)

VA Cooperative Studies Program #571 (DIVA) was designed to evaluate the efficacy of drug-eluting stents (DES) for reducing aortocoronary saphenous vein bypass graft (SVG) failure when compared with bare-metal stents (BMS) in participants undergoing stenting of de novo SVG lesions. Participants undergoing clinically indicated stenting of de novo SVG lesions were randomized in a 1:1 ratio to DES or BMS. Randomization was stratified by presence/absence of diabetes mellitus and the number of target SVG lesions (1 vs ≥2) within each participating site. At sites that did not routinely administer 12-months of dual antiplatelet therapy after SVG stenting participants without acute coronary syndromes received 1 month of open-label clopidogrel, followed by 11 months of clopidogrel for those assigned to DES and 11 months of placebo for those assigned to BMS. The primary endpoint was the 12-month incidence of target-vessel failure (defined as the composite of cardiac death, target-vessel myocardial infarction, or target-vessel revascularization). Secondary endpoints included the incidence of other clinical endpoints and the incremental cost-effectiveness of DES relative to BMS. Due to lower-than-anticipated target-vessel failure rates, target enrollment was increased from 519 to 762. The study had randomized 599 participants when recruitment ended in December 2015. The DIVA trial will provide clarity on the appropriate stent type for de novo SVG lesions.
Time-reversal Operator for the State of Polarization (1993)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text MARIO MARTINELLI, "Time-reversal Operator for the State of Polarization," Optics & Photonics News 4(12), 15-16 (1993) Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
Megakaryocytes Package and Deliver Golgi-Associated Glycosyltransferases into Platelets and to Platelet Surfaces Using Dense Granules. (2005)

Abstract We recently presented evidence for the presence of a β4 galactosyl transferase (β4GalT) on the surface of platelets that catalyzes the coupling of galactose in a β1,4 linkage to exposed N-acetylglucosamine residues on the N-linked glycans of GP1ba and that this reaction improved the circulation of chilled murine platelets. Since an externally-disposed glycosyltransferase activity contradicts the dogma that such enzymes reside solely within the Golgi apparatus, we verified it by demonstrating that intact platelets glycosylate 2.8 μm beads coated with the acceptor substrate GlcNAc-β-R. We then explored the origin of this enzyme in megakaryocytes and its mechanism of delivery to platelets. We report that Golgi marker GM130 as well as a YFP tagged Golgi marker construct based on the polypeptide transferase GalNAc-T2 expressed in cultured mouse megakaryocytes transports from the cell body of to nascent platelets in discrete packets. In immature megakaryocytes, the Golgi organizes, as expected, into perinuclear arrays. However, once proplatelet extension begins, the perinuclear apparatus disassembles, and Golgi-associated proteins transport through the proplatelets in vesicular/granular structures. Labeling with granule markers reveals correspondence of Golgi markers only with dense granules. These findings suggest that Golgi membrane becomes packaged into dense granules, which deliver Golgi-associated glycosyltransferases into the nascent platelets and from there to the platelet surface.