20 IN SITU BIOPRINTING OF SKIN FOR RECONSTRUCTION (2012)

You have accessJournal of UrologyTrauma/Reconstruction: Trauma & Reconstructive Surgery I1 Apr 201220 IN SITU BIOPRINTING OF SKIN FOR RECONSTRUCTION Mohammad Albanna, Sean Murphy, Weixin Zhao, Idris El-Amin, Josh Tan, Dennis Dice, Hyun-Wook Kang, John Jackson, Anthony Atala, and James Yoo Mohammad AlbannaMohammad Albanna Winston Salem, NC More articles by this author , Sean MurphySean Murphy Winston Salem, NC More articles by this author , Weixin ZhaoWeixin Zhao Winston Salem, NC More articles by this author , Idris El-AminIdris El-Amin Winston Salem, NC More articles by this author , Josh TanJosh Tan Winston Salem, NC More articles by this author , Dennis DiceDennis Dice Winston Salem, NC More articles by this author , Hyun-Wook KangHyun-Wook Kang Winston Salem, NC More articles by this author , John JacksonJohn Jackson Winston Salem, NC More articles by this author , Anthony AtalaAnthony Atala Winston Salem, NC More articles by this author , and James YooJames Yoo Winston Salem, NC More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.062AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Extensive reconstructive procedures require grafts for coverage and repair since any loss of full-thickness skin of more than 4 cm in diameter will not heal by itself. Current treatment options such as autografts and commercially available skin products are limited in size and some require a lengthy preparation time, making them unusable. Therefore, a new approach that permits immediate wound stabilization with functional recovery is necessary. We developed a novel skin printer based on bioprinting techniques that would allow for in situ repair of injuries. In this study we investigated whether the skin bioprinter could be used for the repair of large full thickness wounds in a porcine model. METHODS Skin fibroblasts and keratinocytes were isolated from porcine skin. Four full thickness excisional wounds of 10x10 cm each were created on the back of pig model. Autologous and allogenic fibroblasts and keratinocytes, suspended in fibrinogen/ collagen solution, were printed directly on two wounds. Fibroblasts were printed first and crosslinked with thrombin to form a gel layer, followed by deliverying keratinocytes over the fibroblast layer. The remaining two wound groups received fibrinogen/collagen gel without cells and left untreated as controls. The animals were followed for up to 5 weeks and analyzed for wound healing, re-epithelialization and contracture. RESULTS Animals that received autologous cells demonstrated faster wound healing, as compared to other treatment groups. These animals showed approximately 90% reduction in wound size, 80% re-epithelialization and a 40% contracture of original wound size in autologous wounds within the first 3 weeks of the study. Animals treated with allogeneic cells showed comparable wound size, re- epithelialization and wound contracture to the controls. CONCLUSIONS This study shows that the skin bioprinter is able to deliver two different types of skin cells and biomaterials directly on target locations and cover skin defects. Delivery of autologous cells facilitates wound healing and repair of skin defects. This preclinical study suggests that the use of skin bioprinter may be a preferred approach to rapidly cover extensive skin defects for surgical reconstruction. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e8 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.Metrics Author Information Mohammad Albanna Winston Salem, NC More articles by this author Sean Murphy Winston Salem, NC More articles by this author Weixin Zhao Winston Salem, NC More articles by this author Idris El-Amin Winston Salem, NC More articles by this author Josh Tan Winston Salem, NC More articles by this author Dennis Dice Winston Salem, NC More articles by this author Hyun-Wook Kang Winston Salem, NC More articles by this author John Jackson Winston Salem, NC More articles by this author Anthony Atala Winston Salem, NC More articles by this author James Yoo Winston Salem, NC More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...