gebhart/patrepeval huggingface dataset:
1H and 13C NMR study of new organic nitrate esters. I. (2H)‐1,3‐benzoxazin‐4‐(3H)‐one derivatives (1994)

Abstract The 1 H and 13 C NMR of a series of 3‐nitrooxyalkyl‐2 H ‐1,3‐benzoxazin‐4‐(3 H )‐ones were measured and assigned on the basis of substituent chemical shifts, DEPT experiments and 13 C/ 1 H shift correlated spectra.
When plants produce not enough or at all: metabolic engineering of flavonoids in microbial hosts (2015)

As a result of the discovery that flavonoids are directly or indirectly connected to health, flavonoid metabolism and its fascinating molecules that are natural products in plants, have attracted the attention of both the industry and researchers involved in plant science, nutrition, bio/chemistry, chemical bioengineering, pharmacy, medicine, etc. Subsequently, in the past few years, flavonoids became a top story in the pharmaceutical industry, which is continually seeking novel ways to produce safe and efficient drugs. Microbial cell cultures can act as workhorse bio-factories by offering their metabolic machinery for the purpose of optimizing the conditions and increasing the productivity of a selective flavonoid. Furthermore, metabolic engineering methodology is used to reinforce what nature does best by correcting the inadequacies and dead-ends of a metabolic pathway. Combinatorial biosynthesis techniques led to the discovery of novel ways of producing natural and even unnatural plant flavonoids, while, in addition, metabolic engineering provided the industry with the opportunity to invest in synthetic biology in order to overcome the currently existing restricted diversification and productivity issues in synthetic chemistry protocols. In this review, is presented an update on the rationalized approaches to the production of natural or unnatural flavonoids through biotechnology, analyzing the significance of combinatorial biosynthesis of agricultural/pharmaceutical compounds produced in heterologous organisms. Also mentioned are strategies and achievements that have so far thrived in the area of synthetic biology, with an emphasis on metabolic engineering targeting the cellular optimization of microorganisms and plants that produce flavonoids, while stressing the advances in flux dynamic control and optimization. Finally, the involvement of the rapidly increasing numbers of assembled genomes that contribute to the gene- or pathway-mining in order to identify the gene(s) responsible for producing species-specific secondary metabolites is also considered herein.
Zusammenfassung und Ausblick (2004)

Im Mittelpunkt dieser Arbeit steht mit dem NP-schweren Multiprocessor Scheduling-Problem mit reihenfolgeabhängigen Rüstzeiten eine bisher wenig untersuchte Aufgabenstellung. Die Berücksichtigung von reihenfolgeabhängigen Rüstzeiten, die in vielen Praxisanwendungen eine bedeutende Rolle spielen, stellt dabei eine wesentliche Erweiterung der i. a. in der Literatur betrachteten Problemstellungen dar.
An Edge-Combining Frequency-Multiplying Class-D Power Amplifier (2022)

The class-D power amplifier (PA) is commonly implemented in CMOS, but its operating frequency is often limited due to the power loss of parasitic capacitances and the lower transition frequency of the PMOS transistor. In this brief we demonstrate edge-combining frequency-multiplication embedded directly in the output-stage, allowing higher-frequency operation of the class-D PA, while maintaining similar performance to a lower-frequency PA. A 65nm CMOS prototype achieves output power and system efficiency of 22.3dBm and 30.2%, respectively. The prototype is tested with a D-BPSK signal and achieves an EVM of 2%-rms. Although the prototype was not embedded with amplitude modulation capability, it can be readily adapted for such operation using switched-capacitor PA techniques.
Supplementary Table 2 from Cancer-Induced Immunosuppression: IL-18–Elicited Immunoablative NK Cells (2023)

&lt;p&gt;PDF file - 307K, Differentially expressed genes between Kit+ and Kit- samples, coding for annotated genes, FC &gt;2, p-value &lt;0.05, difference of mean &gt; 100&lt;/p&gt;
A 180-220-GHz, 12.7-dBm peak Psat and 17.3% peak PAE Power Amplifier in 250-nm InP HBT (2022)

We present a single-stage G-band power amplifier (PA) with high power density and high efficiency in a 250-nm Indium Phosphide (InP) technology. A pseudo-differential common-base (CB) stage with neutralization capacitors and low-loss coupled-line balun (CLB) are designed to deliver 8.3-12.7-dBm output power and 7.7-17.3% power-added efficiency (PAE) over 180-220 GHz. The use of compact baluns allows the design to occupy only 0.011mm<sup>2</sup> with power density of 1.69 W/mm<sup>2</sup>. The design achieves the highest published PAE and power density above 200 GHz for solid-state PAs.
Efficient automated syntheses of high specific activity 6‐[18F]fluorodopamine using a diaryliodonium salt precursor (2015)

6-[(18)F]Fluorodopamine (6-[(18) F]F-DA) is a positron emission tomography radiopharmaceutical used to image sympathetic cardiac innervation and neuroendocrine tumors. Imaging with 6-[(18)F]F-DA is constrained, in part, by the bioactivity and neurotoxicity of 6-[(19)F]fluorodopamine. Furthermore, routine access to this radiotracer is limited by the inherent difficulty of incorporation of [(18)F]fluoride into electron-rich aromatic substrates. We describe the simple and direct preparation of high specific activity (SA) 6-[(18)F]F-DA from no-carrier-added (n.c.a.) [(18)F]fluoride. Incorporation of n.c.a. [(18)F]fluoride into a diaryliodonium salt precursor was achieved in 50-75% radiochemical yields (decay corrected to end of bombardment). Synthesis of 6-[(18)F]F-DA on the IBA Synthera® and GE TRACERlab FX-FN automated platforms gave 6-[(18)F]F-DA in >99% chemical and radiochemical purities after HPLC purification. The final non-corrected yields of 6-[(18)F]F-DA were 25 ± 4% (n = 4, 65 min) and 31 ± 6% (n = 3, 75 min) using the Synthera and TRACERlab modules, respectively. Efficient access to high SA 6-[(18)F]F-DA from a diaryliodonium salt precursor and n.c.a. [(18)F]fluoride is provided by a relatively subtle change in reaction conditions - replacement of a polar aprotic solvent (acetonitrile) with a relatively nonpolar solvent (toluene) during the critical radiofluorination reaction. Implementation of this process on common radiochemistry platforms should make 6-[(18)F]F-DA readily available to the wider imaging community.
Performances RF de circuits MIDs réalisés par LDS (2013)

National audience
Aging and DNA methylation in colorectal mucosa and cancer. (1998)

DNA methylation of promoter-associated CpG islands may function as an alternate mechanism of silencing tumor suppressor genes in multiple neoplasias including colorectal cancer. De novo methylation of genes appears to be an early and frequent event in most neoplasias. For the ER and IGF2 genes, we have previously shown that methylation actually begins in the normal colon mucosa as an age-related event and progresses to hypermethylation in cancer. In this study, we have determined the frequency of age-related methylation in normal colonic mucosa among the genes hypermethylated in colorectal cancer. We studied six genes, including N33, MYOD, p16, HIC-1, THBS1, and CALCA. The N33 gene showed partial methylation in normal colon mucosa, which was age-related (r = 0.7; P = 0.003 using regression analysis). Adenomas and cancers showed further hypermethylation at this locus. Similarly, the MYOD gene showed age-related methylation in normal colon mucosa (r = 0.7; P < 0.00001 using regression analysis) and hypermethylation in cancers. Age-related methylation seems to be gene specific, because p16, THBS1, HIC-1, and CALCA were not affected. Furthermore, this process may also be modulated by tissue-specific factors. Our study suggests that aging is a major contributing factor to hypermethylation in cancer.
Direct Measurements of Volumetric Gas Storage Capacity and Some New Insight into Adsorbed Natural Gas Storage (2001)

We present a unique bench scale apparatus for directly measuring volumetric gas storage capacities designed at UTRC. The apparatus construction avoids gas leakage, and the analysis of experimental data prevents leakage from inflating capacity measurements. Deliverable methane storage capacities (Vd/Vs) of adsorbents are evaluated directly from experiments with this apparatus rather than calculated from gravimetric adsorption capacities. We suggest that an adsorbent for methane storage should have an optimal pore volume consisting of pores ranging from 8 to 15 Å, rather than a monodispersed 8 Å pore size distribution as calculated by recent computer simulations. This interpretation is based on our modeling results and on the fact that physical activation usually produces an adsorbent with a polydisperse distribution of pore size. In general, ultra-micropores (<7 Å) should be avoided and super-micropores (7−20 Å) should be developed during adsorbent preparation. Ultra-micropores collect “cushion gas,” thus reducing deliverable storage capacity. Super-micropores offer high methane adsorption densities and fast adsorption/desorption kinetics.
Developmental and adult expression patterns of the G‐protein‐coupled receptor GPR88 in the rat: Establishment of a dual nuclear–cytoplasmic localization (2016)

GPR88 is a neuronal cerebral orphan G-protein-coupled receptor (GPCR) that has been linked to various psychiatric disorders. However, no extensive description of its localization has been provided so far. Here, we investigate the spatiotemporal expression of the GPR88 in prenatal and postnatal rat tissues by using in situ hybridization and immunohistochemistry. GPR88 protein was initially detected at embryonic day 16 (E16) in the striatal primordium. From E16-E20 to adulthood, the highest expression levels of both protein and mRNA were observed in striatum, olfactory tubercle, nucleus accumbens, amygdala, and neocortex, whereas in spinal cord, pons, and medulla GPR88 expression remains discrete. We observed an intracellular redistribution of GPR88 during cortical lamination. In the cortical plate of the developing cortex, GPR88 presents a classical GPCR plasma membrane/cytoplasmic localization that shifts, on the day of birth, to nuclei of neurons progressively settling in layers V to II. This intranuclear localization remains throughout adulthood and was also detected in monkey and human cortex as well as in the amygdala and hypothalamus of rats. Apart from the central nervous system, GPR88 was transiently expressed at high levels in peripheral tissues, including adrenal cortex (E16-E21) and cochlear ganglia (E19-P3), and also at moderate levels in retina (E18-E19) and spleen (E21-P7). The description of the GPR88 anatomical expression pattern may provide precious functional insights into this novel receptor. Furthermore, the GRP88 nuclear localization suggests nonclassical GPCR modes of action of the protein that could be relevant for cortical development and psychiatric disorders. J. Comp. Neurol. 524:2776-2802, 2016. © 2016 Wiley Periodicals, Inc.
Hippocampal and Entorhinal Cortex Neurofibrillary Tangle Formation in Guamanian Chamorros Free of Overt Neurologic Dysfunction (2003)

Since first described, amyotrophic lateral sclerosis/parkinsonism-dementia complex (ALS/PDC) of Guam has represented an important model of age-related neurodegenerative disease. ALS/PDC is characterized neuropathologically by severe widespread involvement by neurofibrillary tangles (NFTs). Over the past 30 years there has been a dramatic decrease in the incidence of ALS and a 10-year increase in the age of onset of ALS and PDC. In 1979, Anderson et al reported evidence of significant NFT involvement in Guam natives who had been free of evidence of neurologic dysfunction. Using the slides from this study, we re-examined the extent of hippocampus and entorhinal NFT involvement and compared it to brains recently obtained from neurologically intact Guam natives and age-matched controls from New York. The tendency towards hippocampal and entorhinal NFT formation continues to be encountered among the inhabitants of Guam, particularly among those over age 50. although severe involvement was less commonly noted in relatively young individuals (< 50 years). As noted by Anderson et al, the pattern of neuropathologic lesions seen in those with extensive NFT involvement suggests that such cases represent preclinical examples of ALS/PDC in individuals who have yet to accumulate a sufficient burden of pathology to attract clinical attention and diagnostic evaluation.
ChemInform Abstract: Synthesis of a Dihydropyrano(3,2‐e)indole as a Rotationally Restricted Phenolic Analog of the Neurotransmitter Serotonin. (1992)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
An energy-efficient VLSI architecture for pattern recognition via deep embedding of computation in SRAM (2014)

In this paper, we propose the concept of compute memory, where computation is deeply embedded into the memory (SRAM). This deep embedding enables multi-row read access and analog signal processing. Compute memory exploits the relaxed precision and linearity requirements of pattern recognition applications. System-level simulations incorporating various deterministic errors from analog signal chain demonstrates the limited accuracy of analog processing does not significantly degrade the system performance, which means the probability of pattern detection is minimally impacted. The estimated energy saving is 63 % as compared to the conventional system with standard embedded memory and parallel processing architecture, for 256×256 target image.
A Dual CuH- and Pd-Catalyzed Stereoselective Synthesis of Highly Substituted 1,3-Dienes (2021)

Conjugated dienes are versatile building blocks and prevalent substructures in synthetic chemistry. Herein, we report a method for the stereoselective hydroalkenylation of alkynes, utilizing readily available enol triflates. We leveraged an <i>in situ</i>-generated and geometrically pure vinyl-Cu(I) species to form the <i>Z</i>,<i>Z</i>- or <i>Z</i>,<i>E</i>-1,3-dienes in excellent stereoselectivity and yield. This approach allowed for the synthesis of highly substituted <i>Z</i>-dienes, including pentasubstituted 1,3-dienes, which are difficult to prepare by existing approaches.
640-Gbit/s Data Transmission and Clock Recovery Using an Ultrafast Periodically Poled Lithium Niobate Device (2009)

This paper presents the first demonstration of the use of a periodically poled lithium niobate device for signal processing at 640 Gbit/s. Clock recovery is performed successfully using the lithium niobate device, and the clock signal is used to control a nonlinear fiber-based demultiplexer. The full 640-Gbit/s system gives error-free performance with no pattern dependence and there is less than 1-dB power penalty after 50-km fiber transmission.
Modular microfluidic system fabricated in thermoplastics for the strain-specific detection of bacterial pathogens (2020)

The recent outbreaks of a lethal E. coli strain in Germany have aroused renewed interest in developing rapid, specific and accurate systems for detecting and characterizing bacterial pathogens in suspected contaminated food and/or water supplies. To address this need, we have designed, fabricated and tested an integrated modular-based microfluidic system and the accompanying assay for the strain-specific identification of bacterial pathogens. The system can carry out the entire molecular processing pipeline in a single disposable fluidic cartridge and detect single nucleotide variations in selected genes to allow for the identification of the bacterial species, even its strain with high specificity. The unique aspect of this fluidic cartridge is its modular format with task-specific modules interconnected to a fluidic motherboard to permit the selection of the target material. In addition, to minimize the amount of finishing steps for assembling the fluidic cartridge, many of the functional components were produced during the polymer molding step used to create the fluidic network. The operation of the cartridge was provided by electronic, mechanical, optical and hydraulic controls located off-chip and packaged into a small footprint instrument (1 ft3). The fluidic cartridge was capable of performing cell enrichment, cell lysis, solid-phase extraction (SPE) of genomic DNA, continuous flow (CF) PCR, CF ligase detection reaction (LDR) and universal DNA array readout. The cartridge was comprised of modules situated on a fluidic motherboard; the motherboard was made from polycarbonate, PC, and used for cell lysis, SPE, CF PCR and CF LDR. The modules were task-specific units and performed universal zip-code array readout or affinity enrichment of the target cells with both made from poly(methylmethacrylate), PMMA. Two genes, uidA and sipB/C, were used to discriminate between E. coli and Salmonella, and evaluated as a model system. Results showed that the fluidic system could successfully identify bacteria in <40 min with minimal operator intervention and perform strain identification, even from a mixed population with the target of a minority. We further demonstrated the ability to analyze the E. coli O157:H7 strain from a waste-water sample using enrichment followed by genotyping.
Pressure-induced superconductivity and topological quantum phase transitions in a quasi-one-dimensional topological insulator: Bi4I4 (2018)

Abstract Superconductivity and topological quantum states are two frontier fields of research in modern condensed matter physics. The realization of superconductivity in topological materials is highly desired; however, superconductivity in such materials is typically limited to two-dimensional or three-dimensional materials and is far from being thoroughly investigated. In this work, we boost the electronic properties of the quasi-one-dimensional topological insulator bismuth iodide β-Bi 4 I 4 by applying high pressure. Superconductivity is observed in β-Bi 4 I 4 for pressures, where the temperature dependence of the resistivity changes from a semiconducting-like behavior to that of a normal metal. The superconducting transition temperature T c increases with applied pressure and reaches a maximum value of 6 K at 23 GPa, followed by a slow decrease. Our theoretical calculations suggest the presence of multiple pressure-induced topological quantum phase transitions as well as a structural–electronic instability.
Regulation of Rhodopsin Dephosphorylation by Arrestin (1989)

We have characterized the opsin phosphatase activities in extracts of rod outer segments and determined their relationship to known protein phosphatases. The opsin phosphatase activity in the extracts was not due to protein phosphatases 1, 2B, or 2C because it was neither stimulated by Mg2+ or Ca2+/calmodulin nor inhibited by protein phosphatase inhibitors-1 or -2. Opsin phosphatase activity in rod outer segment extracts was potently inhibited by okadaic acid (IC50 approximately 10 nM), a preferential inhibitor of protein phosphatase 2A. Moreover, during chromatography on DEAE-Sepharose, the opsin phosphatase activity co-eluted with three peaks of protein phosphatase 2A activity, termed protein phosphatases 2A0, 2A1, and 2A2. The opsin phosphatase activity of each peak was stimulated by polylysine, a known activator of protein phosphatase 2A. Finally, treatment of rod outer segment extracts with 80% ethanol at room temperature converted the activity from a high molecular weight form characteristic of the protein phosphatase 2A0, 2A1, and 2A2 species to a low molecular weight form characteristic of the protein phosphatase 2A catalytic subunit. We conclude that protein phosphatase 2A is likely to be the physiologically relevant rhodopsin phosphatase. The 48-kDa rod outer segment protein arrestin (S-antigen) was found to inhibit the dephosphorylation of freshly photolyzed rhodopsin by protein phosphatase 2A but did not inhibit the dephosphorylation of unbleached rhodopsin. Arrestin has no effect on the dephosphorylation of phorphorylase a, indicating that the effect was substrate-directed. It appears that dephosphorylation of the photoreceptor protein phosphorhodopsin occurs only after decay of the photoactivated protein and that this may be regulated in vivo by arrestin. The binding of arrestin to photolyzed phosphorylated rhodopsin, i.e. the binding of a regulatory protein to a protein phosphatase substrate to form a complex resistant to dephosphorylation represents a novel mechanism for the regulation of protein phosphatase 2A.
Selectivity control in hydrogenation through adaptive catalysis using ruthenium nanoparticles on a CO2-responsive support (2021)

With the advent of renewable carbon resources, multifunctional catalysts are becoming essential to hydrogenate selectively biomass-derived substrates and intermediates. However, the development of adaptive catalytic systems, that is, with reversibly adjustable reactivity, able to cope with the intermittence of renewable resources remains a challenge. Here, we report the preparation of a catalytic system designed to respond adaptively to feed gas composition in hydrogenation reactions. Ruthenium nanoparticles immobilized on amine-functionalized polymer-grafted silica act as active and stable catalysts for the hydrogenation of biomass-derived furfural acetone and related substrates. Hydrogenation of the carbonyl group is selectively switched on or off if pure H<sub>2</sub> or a H<sub>2</sub>/CO<sub>2</sub> mixture is used, respectively. The formation of alkylammonium formate species by the catalytic reaction of CO<sub>2</sub> and H<sub>2</sub> at the amine-functionalized support has been identified as the most likely molecular trigger for the selectivity switch. As this reaction is fully reversible, the catalyst performance responds almost in real time to the feed gas composition.
CAD-based line/space mix-up prevention for reticle metrology (2007)

This paper describes a method to automatically distinguish between line and space for 1:1 line space patterns in mask metrology. As the number of measurements typically performed on a reticle is significantly higher than on a wafer, automated CAD based CD-SEM recipe creation is essential. Such recipes typically use synthetic pattern recognition targets instead of SEM based pattern recognition targets. Therefore, a possible different contrast between lines and spaces on a mask cannot be utilized for distinguishing lines from spaces. We demonstrate an algorithm solution based on the analysis of the SEM waveform profiles to identify potential L/S mix-ups and correct them automatically. The solution allows fully automated CAD based offline recipe creation with a high success rate of distinction between lines and spaces for 1:1 pitch cases without the necessity of editing recipes on the tool in advance of performing the measurements.