gebhart/patrepeval huggingface dataset:
Selection for CD49A+ and CD26- cells in pancreatic islet-like clusters differentiated from human pluripotent stem cells improves their therapeutic activity in diabetic mice (2020)

<title>Abstract</title> BackgroundCell therapy of diabetes aims at restoring the physiological control of blood glucose by transplantation of functional pancreatic islet cells. Human islets from post-mortem donations have shown efficiency but the demand for islets vastly exceeds the availability of donations. A potentially unlimited source of cells for such transplantations would be islet cells derived from in vitro differentiation of human pluripotent stem cells (hPSC), such as embryonic stem cells (hESC). The islet-like clusters (ILC) produced by the known differentiation protocols contain various cell populations. Among these, the beta cells that express both insulin and the transcription factor Nkx6.1 seem to be the most efficient to restore normoglycemia in diabetes animal models. Our aim was to find markers allowing selection of these efficient cells.MethodsFunctional Cell-Capture Screening (FCCS), using an array of antibodies to cell surface proteins, was used to identify markers that preferentially capture the cells expressing insulin, or expressing both insulin and Nkx6.1, from hESC-derived ILC cells. In order to test whether selection for such markers could improve cell therapy in diabetic mouse models, we used ILC produced from a clinical-grade line of hESC by a refined differentiation protocol adapted to up-scalable bioreactors. The ILC, dissociated to single cells, were fractionated by Magnetic Activated Cell Sorting (MACS) for presence of the marker. The sorted cells, re-aggregated into clusters, were encapsulated in microspheres made of alginate modified to reduce foreign body reaction. Implantation was done intraperitoneally in C57BL/6 immuno-competent mice that were made diabetic by prior injections of Streptozotocin (STZ).ResultsCD49A (integrin alpha1) was identified by FCCS as a marker for cells double positive (DP) for insulin (and C-peptide) as well as Nkx6.1 in ILC derived by hESC differentiation. After sorting by MACS with CD49A antibodies, the ILC fraction enriched in CD49A<sup><bold>+</bold></sup> cells rapidly reduced glycemia when implanted in the diabetic mice, whereas mice receiving the CD49A depleted population remained highly diabetic. CD49A-enriched ILC cells also produced significantly higher levels of human C-peptide in mouse blood. Another marker, CD26 (DPP4, dipeptidyl peptidase-4), was identified by FCCS as binding insulin-expressing cells which are Nkx6.1-negative. Depletion of CD26<sup><bold>+</bold></sup> cells followed by enrichment for CD49A<sup><bold>+</bold></sup> cells increased DP cells to over 70%. After this double selection, the CD26 depleted/CD49A enriched ILC were more active than non-sorted ILC to reduce glycemia in the diabetic mice.ConclusionsRefining the composition of ILC differentiated from hPSC by negative selection to remove cells expressing CD26 and positive selection for CD49A expressing cells can enable more effective cell therapy of diabetes.
PrivateMail: Supervised Manifold Learning of Deep Features with Privacy for Image Retrieval (2022)

Differential Privacy offers strong guarantees such as immutable privacy under any post-processing. In this work, we propose a differentially private mechanism called PrivateMail for performing supervised manifold learning. We then apply it to the use case of private image retrieval to obtain nearest matches to a client’s target image from a server’s database. PrivateMail releases the target image as part of a differentially private manifold embedding. We give bounds on the global sensitivity of the manifold learning map in order to obfuscate and release embeddings with differential privacy inducing noise. We show that PrivateMail obtains a substantially better performance in terms of the privacy-utility trade off in comparison to several baselines on various datasets. We share code for applying PrivateMail at http://tiny.cc/PrivateMail.
Introduction to the “Recent Advances in f-Element Organometallic Chemistry” Special Issue of Organometallics (2013)

ADVERTISEMENT RETURN TO ISSUEEditor's PageNEXTIntroduction to the "Recent Advances in f-Element Organometallic Chemistry" Special Issue of OrganometallicsTobin J. MarksView Author Information Northwestern UniversityCite this: Organometallics 2013, 32, 5, 1133–1136Publication Date (Web):March 11, 2013Publication History Published online11 March 2013Published inissue 11 March 2013https://pubs.acs.org/doi/10.1021/om400094nhttps://doi.org/10.1021/om400094neditorialACS PublicationsCopyright © 2013 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views3565Altmetric-Citations10LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (679 KB) Get e-AlertscloseSUBJECTS:Hydrocarbons,Lanthanides and actinides,Ligands,Metals,Organometallic chemistry Get e-Alerts
ERG Induces Epigenetic Activation of Tudor Domain-Containing Protein 1 (TDRD1) in ERG Rearrangement-Positive Prostate Cancer (2013)

We demonstrate that ERG is capable of disrupting a tissue-specific DNA methylation pattern at the TDRD1 promoter. As a result, TDRD1 becomes transcriptionally activated in TMPRSS2:ERG-positive prostate cancer. Given the prevalence of ERG fusions, TDRD1 overexpression is a common alteration in human prostate cancer which may be exploited for diagnostic or therapeutic procedures.
High-Directivity Microstrip Patch Antennas Based on TModd-0 Modes (2019)

High-directivity microstrip antennas are attractive for the line-of-sight communications due to their thin profile. In order to achieve a high-directive radiation pattern, the geometry of a patch operating at a TM <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">odd-0</sub> mode is modified to minimize the effect of out-of-phase currents. Two high-directivity microstrip patch antennas operating at modified TM <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">30</sub> and TM <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">50</sub> modes are presented. They exhibit broadside radiation patterns with directivities of 15 and 18 dBi, respectively. The advantage of the proposed technique compared to an antenna array is that no feeding network is required, and thus, the design is simpler and avoids the losses caused by the feeding network. A prototype to validate the proposed technique is built, achieving a measured directivity of 14.6 dBi. The microstrip patch antenna has a total size of 3(λ/2) × 3(λ/2), and its measured bandwidth is four times that of an equivalent array of microstrip patches operating in its fundamental mode.
Differential Effects of Angiotensin II Type-1 Receptor Antisense Oligonucleotides on Renal Function in Spontaneously Hypertensive Rats (2005)

The effect of selectively decreasing renal angiotensin II type 1 (AT1) receptor expression on renal function and blood pressure has not been determined. Therefore, we studied the consequences of selective renal inhibition of AT1 receptor expression in normotensive Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) in vivo. Vehicle, AT1 receptor antisense oligodeoxynucleotides (AS-ODN), or scrambled oligodeoxynucleotides were infused chronically into the cortex of the remaining kidney of conscious, uninephrectomized WKY and SHR on a 4% NaCl intake. Basal renal cortical membrane AT1 receptor protein was greater in SHR than in WKY. In WKY and SHR, AS-ODN decreased renal but not cardiac AT1 receptors. AT1 receptor AS-ODN treatment increased plasma renin activity to a greater extent in WKY than in SHR. However, plasma angiotensin II and aldosterone were increased by AS-ODN to a similar degree in both rat strains. In SHR, sodium excretion was increased and sodium balance was decreased by AS-ODN but had only a transient ameliorating effect on blood pressure. Urinary protein and glomerular sclerosis were markedly reduced by AS-ODN-treated SHR. In WKY, AS-ODN had no effect on sodium excretion, blood pressure, or renal histology but also modestly decreased proteinuria. The major consequence of decreasing renal AT1 receptor protein in the SHR is a decrease in proteinuria, probably as a result of the amelioration in glomerular pathology but independent of systemic blood pressure and circulating angiotensin II levels.
Supplemental Figure 1 from A Systematic Analysis Reveals Heterogeneous Changes in the Endocytic Activities of Cancer Cells (2023)

&lt;p&gt;Supplemental Figure 1: TfnR and CD8 internalization as measured by In-Cell ELISA.&lt;/p&gt;
Study of 3D process impact on advanced CMOS devices (2013)

Through 3D-IC Integration is possible to put more transistors on the same footprint without the need to shrink transistor sizes. As for any new technology, there are many challenges and issues that need to be addressed before moving to high volume manufacturing. In this work we introduce the several processes to generate 3D stacked devices. We focus on processes like TSV, wafer thinning, backside passivation, back side RDL (Re-Distribution Layer), front side and backside μbumping. We report on the characterization of the effects on transistor devices due to TSV proximity, wafer thinning and die stacking. The transistor devices are based on imec CMOS technology and are manufactured on 300mm diameter wafers. The wafers are fully processed with Front End of Line (FEOL), Back End Of Line (BEOL), wafer thinning on Si carrier, back-side wafer bumping, carrier de-bonding, dicing and final 3D-stacking. We report the main electrical characterizations done to identify the impact of typical 3D processes on CMOS devices. The characterizations are performed in-line (clean room compatible test equipment) and confirmed offline (standard testing equipment out of clean room environment). To characterize the process steps of interest, we use dedicated test structures that are measured before and after the process. The dedicated test structures are mainly measured after wafer front side processing (FEOL and BEOL) and tested again after wafer thinning and after die stacking. The measured variations (typically ION currents) are later elaborated to illustrate the process effect. In case of 3D stacks, the characterization is executed for two-dies stacking. To have an assessment of the 3D stacking yield, the dies are connected by TSVs-μbumps in several daisy chain configurations.
Mimicking tumor hypoxia and tumor-immune interactions employing three-dimensional in vitro models (2020)

The heterogeneous tumor microenvironment (TME) is highly complex and not entirely understood. These complex configurations lead to the generation of oxygen-deprived conditions within the tumor niche, which modulate several intrinsic TME elements to promote immunosuppressive outcomes. Decoding these communications is necessary for designing effective therapeutic strategies that can effectively reduce tumor-associated chemotherapy resistance by employing the inherent potential of the immune system.While classic two-dimensional in vitro research models reveal critical hypoxia-driven biochemical cues, three-dimensional (3D) cell culture models more accurately replicate the TME-immune manifestations. In this study, we review various 3D cell culture models currently being utilized to foster an oxygen-deprived TME, those that assess the dynamics associated with TME-immune cell penetrability within the tumor-like spatial structure, and discuss state of the art 3D systems that attempt recreating hypoxia-driven TME-immune outcomes. We also highlight the importance of integrating various hallmarks, which collectively might influence the functionality of these 3D models.This review strives to supplement perspectives to the quickly-evolving discipline that endeavors to mimic tumor hypoxia and tumor-immune interactions using 3D in vitro models.
Characterizing Protein Glycosylation through On-Chip Glycan Modification and Probing (2016)

Glycans are critical to protein biology and are useful as disease biomarkers. Many studies of glycans rely on clinical specimens, but the low amount of sample available for some specimens limits the experimental options. Here we present a method to obtain information about protein glycosylation using a minimal amount of protein. We treat proteins that were captured or directly spotted in small microarrays (2.2 mm × 2.2 mm) with exoglycosidases to successively expose underlying features, and then we probe the native or exposed features using a panel of lectins or glycan-binding reagents. We developed an algorithm to interpret the data and provide predictions about the glycan motifs that are present in the sample. We demonstrated the efficacy of the method to characterize differences between glycoproteins in their sialic acid linkages and N-linked glycan branching, and we validated the assignments by comparing results from mass spectrometry and chromatography. The amount of protein used on-chip was about 11 ng. The method also proved effective for analyzing the glycosylation of a cancer biomarker in human plasma, MUC5AC, using only 20 μL of the plasma. A glycan on MUC5AC that is associated with cancer had mostly 2,3-linked sialic acid, whereas other glycans on MUC5AC had a 2,6 linkage of sialic acid. The on-chip glycan modification and probing (on-chip GMAP) method provides a platform for analyzing protein glycosylation in clinical specimens and could complement the existing toolkit for studying glycosylation in disease.
Robot-assisted delayed extraction of retained Lasso catheter combined with mitral valve repair and arrhythmia ablation (2015)

Atrial fibrillation (AF) is a common rhythm disturbance that increases in prevalence with age. While pharmacologic agents are routinely utilized as first line therapy, catheter ablation of AF is often recommended when medications are unsuccessful or not appropriate. While infrequent entrapment of the circular catheter in the mitral apparatus has been reported, requiring emergent cardiac surgery via sternotomy.1 We report delayed removal of the entrapped catheter along with mitral repair and completion of arrhythmia ablation using a minimally invasive robot-assisted approach.
Heterobi‐ and ‐trimetallic Ion Pairs of Zirconocene‐Based Isoselective Olefin Polymerization Catalysts with AlMe3 (2015)

The reactivity towards AlMe3 of discrete cationic ansa-zirconocenes 2 a,b that are ubiquitously used in isoselective propylene polymerization and based on [{Ph(H)C(3,6-tBu2-Flu)(3-tBu-5-Et-Cp)}ZrMe2)] {Cp-Flu} and rac-[{Me2Si-(2-Me-4-Ph-Ind)2}ZrMe2] {SBI} was scrutinized. The first example of a structurally characterized Group 4 metallocene AlMe3 adduct (3 b) is reported. In the presence of excess AlMe3, the {SBI}-based AlMe3 adduct 3 b undergoes a slow decomposition via C-H activation in a bridging methyl unit to yield a new species (4 b) with a trimetallic {Zr(μ-CH2)(μ-Me)AlMe(μ-Me)AlMe2} core. EXSY NMR data for the process 2 b⇄3 b→4 b suggest very rapid and reversible binding of an additional AlMe3 molecule onto AlMe3 adduct 3 b. The resulting heterotrimetallic species intermediates exchange of methyl groups between different metal centers and slowly undergoes the C-H activation reaction towards 4 b.
Supplementary Data from HER3 Is a Determinant for Poor Prognosis in Melanoma (2023)

Supplementary Data from HER3 Is a Determinant for Poor Prognosis in Melanoma
All-semiconductor metamaterial with negative refraction in the near-infrared (2012)

When heavily doped, semiconductors such as ZnO can exhibit metallic properties thus becoming versatile building blocks for optical metamaterials. Here, we design and fabricate an all-semiconductor metamaterial and demonstrate negative refraction in the near-infrared region.
ChemInform Abstract: Friedel‐Crafts‐Type Reactions Involving Di‐ and Tricationic Species. Onium‐Allyl Dications and O,O‐Diprotonated aci‐Nitro Species Bearing a Protonated Carbonyl Group. (1991)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
A High Performance and Energy-Efficient Cold Data Eviction Algorithm for 3D-TSV Hybrid ReRAM/MLC NAND SSD (2013)

A hybrid 3D-TSV ReRAM/MLC NAND SSD with cold data eviction (CDE) algorithm is proposed. In the proposed hybrid SSD, the lifetime and energy consumption are dominated by MLC NAND flash memory due to ReRAM's high endurance and low power consumption. In addition, partial page overwrites are possible in ReRAM. Thus, the write accesses to MLC NAND flash memory are largely reduced by storing hot data in ReRAM. As a result, the SSD energy consumption decreases and the lifetime is prolonged. With the CDE algorithm, a page-level adaptive data migration is achieved, which is transparent to the file system. Compared to the previous work, 8-times write throughput increase, 83% energy reduction and 6.5-times longer longevity are achieved with 3D-TSV technology. Moreover, from the experimental results, the data eviction should be triggered when ReRAM free space ratio decreases to a range of 8%-20%. Hence, the eviction frequency is adaptive to the data pattern in the hybrid SSD. The experimental results also suggest the requirements for ReRAM. To obtain the best effect, both the read and write latency of ReRAM should be below 3 μs for 512 Bytes.
Structure Determination of N‐Methyl‐tetrahydro‐5H‐indazol‐5‐ones. (2006)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Carbon Source-dependent Assembly of the Snf1p Kinase Complex inCandidaalbicans (2005)

The Snf1p/AMP-activated kinases are involved in transcriptional, metabolic, and developmental regulation in response to stress. In Saccharomyces cerevisiae, Snf1p (Cat1p) is one of the key regulators of carbohydrate metabolism, and cat1 (snf1) mutants fail to grow with non-fermentable carbon sources. In Candida albicans, Snf1p is an essential protein and cells depend on a functional Snf1 kinase even with glucose as carbon source. We investigated the CaSnf1p complex after tandem affinity purification and mass spectrometric analysis and show that the complex composition changes with the carbon source provided. Three subunits were identified, one of which was named CaSnf4p because of its homology to the ScSnf4 protein and the respective CaSNF4 gene could complement a S. cerevisiae snf4 mutant. The other two proteins revealed similarities to the S. cerevisiae kinase beta subunits ScGal83p, ScSip2p, and ScSip1p. Both genes complemented the scaffold function in a S. cerevisiae gal83,sip1,sip2 triple deletion mutant and were named according to their scaffold function as CaKIS1p and CaKIS2p. Matrix-assisted laser desorption ionization peptide mass fingerprint analysis indicated that CaKis2p is N-terminal myristoylated and the incorporation of CaKis2p in the Snf1p complex was reduced when compared with cells grown with glucose as a carbon source. To verify the different complex assemblies, a stable isotope labeling technique (iTraqtrade mark) was employed, confirming a 3-fold decrease of CaKis2p with ethanol. Yeast two-hybrid analysis confirmed the interaction partners, and these results showed an activator domain for the CaKis2 protein that has not been reported for S. cerevisiae scaffold subunits.
Overview on initial METIS D2D Concept (2014)

METIS is the EU flagship project with the purpose of developing a 5G system concept that meets the requirements of the beyond-2020 connected information society and emerging applications. Within METIS project, horizontal topics (HTs) are used to build the overall system concept. Direct Device-to-Dev
Effect of Agglomerate Microstructure on Oxygen Reduction in Catalyst Layers of Polymer Electrolyte Fuel Cells (2012)

Cathode catalyst layers (CCLs) contribute to a major proportion, 30%–40%, of voltage losses in Polymer Electrolyte fuel cells (PEFC). The objectives of the present study are to investigate how the catalyst layer performance depends on electrostatic interaction between reacting protons and the charged metal phase and how these relations are affected by composition and microstructure of agglomerates. A model is developed to study oxygen reduction in catalyst layers based on a novel agglomerate microstructure with conical pores. The model consists of coupled relations for reactant transport, metal charging behaviour, and interfacial charge transfer kinetics, evaluated under steady state conditions. Results show an enhancement in the effectiveness factor of conical pores compared to cylindrical pores. Results of the model are evaluated by comparison with existing experimental data. The effectiveness factor calculated for catalyst layer is 3% which is comparable to existing experimental data.
Exploiting semantic annotations in information retrieval (2008)

The following report summarizes the highlights of the first workshop on exploiting semantic annotations in information retrieval (ESAIR '08). The workshop format included paper and demo presentations as well as breakout sessions and a panel discussion.
Design and Evaluation of primitives for Parallel I/O (1993)

Article Design and Evaluation of primitives for Parallel I/O Share on Authors: R. Bordawekar Northeast Parallel Architectures Center, 3-201 CST, Syracuse Univ., Syracuse, NY Northeast Parallel Architectures Center, 3-201 CST, Syracuse Univ., Syracuse, NYView Profile , J. M. del Rosario Northeast Parallel Architectures Center, 3-201 CST, Syracuse Univ., Syracuse, NY Northeast Parallel Architectures Center, 3-201 CST, Syracuse Univ., Syracuse, NYView Profile , A. Choudhary Northeast Parallel Architectures Center, 3-201 CST, Syracuse Univ., Syracuse, NY and ECE Dept. Northeast Parallel Architectures Center, 3-201 CST, Syracuse Univ., Syracuse, NY and ECE Dept.View Profile Authors Info & Claims Supercomputing '93: Proceedings of the 1993 ACM/IEEE conference on SupercomputingDecember 1993 Pages 452–461https://doi.org/10.1145/169627.169782Online:01 December 1993Publication History 71citation227DownloadsMetricsTotal Citations71Total Downloads227Last 12 Months5Last 6 weeks1 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access