gebhart/patrepeval huggingface dataset:
Crystallization and preliminary X-ray crystallographic studies of an oligomeric species of a refolded C39 peptidase-like domain of theEscherichia coliABC transporter haemolysin B (2011)

The ABC transporter haemolysin B (HlyB) from Escherichia coli is part of a type I secretion system that translocates a 110 kDa toxin in one step across both membranes of this Gram-negative bacterium in an ATP-dependent manner. Sequence analysis indicates that HlyB contains a C39 peptidase-like domain at its N-terminus. C39 domains are thiol-dependent peptidases that cleave their substrates after a GG motif. Interestingly, the catalytically invariant cysteine is replaced by a tyrosine in the C39-like domain of HlyB. Here, the overexpression, purification and crystallization of the isolated C39-like domain are described as a first step towards obtaining structural insights into this domain and eventually answering the question concerning the function of a degenerated C39 domain in the ABC transporter HlyB.
Adaptable architectures for distributed visual target tracking (2011)

There are a growing number of visual tracking applications for mobile devices. However, the computer vision algorithms which process real-time video to track moving targets are demanding. Since a single mobile device possesses limited computational capabilities, energy, etc. to fully support target tracking, some works have investigated architectures which migrate a portion of tracking duties to another device at the cost of transmission bandwidth and energy. In this paper, we investigate the resource utilization in such architectures and present an adaptable architecture which balances tracking workload among the participating devices based on current resource availability (energy, temperature, bandwidth). Results show that the proposed solution requires low additional overhead, can improve on tracking system lifetime by reducing energy consumption, and is more effective in maintaining safe operating temperatures within participants as compared to previously investigated architecture
Novel formal [3 + 2] annulation reaction based on S-propargyl dithiocarbonates (xanthates) (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNovel formal [3 + 2] annulation reaction based on S-propargyl dithiocarbonates (xanthates)Jean Boivin, Catherine Tailhan, and Samir Z. ZardCite this: J. Am. Chem. Soc. 1991, 113, 15, 5874–5876Publication Date (Print):July 1, 1991Publication History Published online1 May 2002Published inissue 1 July 1991https://pubs.acs.org/doi/10.1021/ja00015a058https://doi.org/10.1021/ja00015a058research-articleACS PublicationsRequest reuse permissionsArticle Views357Altmetric-Citations20LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Nanowindow-Induced Molecular Sieving Effect in a Single-Wall Carbon Nanohorn (2002)

Subnanoscale windows (nanowindows) were donated to the walls of single-wall carbon nanohorns (SWNHs) by oxidation in oxygen at different temperatures of 573−693 K. We determined the accessibility of internal nanospaces of the SWNHs through nanowindows to He, Ar, N2, CH4, SF6, and C60. An explicit molecular sieving effect of SWNHs due to the nanowindow was shown.
High-Definition IPTV Broadcasting Architecture Over Gigabit-Capable Passive Optical Network (2007)

Service providers are currently seeking to offer high-quality IP video service on single broadband IP network infrastructures, including ADSL and passive optical networks (PON). One of motivation of our work is increasing the bandwidth utilization efficiency of video data delivery to home subscribers and reducing the channel zapping time. We propose a high-definition IPTV broadcasting architecture over GPON that can provide 100 HDTV channel over fiber lines in this paper consisting of a Gigabit-capable passive optical networks (GPON), IPTV terminals, an IP broadcasting server, and an IP broadcasting management server. We adapt the optical broadcast nature of a GPON to IP multicast networks to effectively broadcast video content to home subscribers. We develop IPTV channel selection mechanism in the GPON ONT by selecting a multicast stream at a subscriber's home that can achieve a 10-msec IPTV channel selection speed.
Histidine-rich glycoprotein promotes bacterial entrapment in clots and decreases mortality in a mouse model of sepsis (2010)

Streptococcus pyogenes is a significant bacterial pathogen in humans. In this study, histidine-rich glycoprotein (HRG), an abundant plasma protein, was found to kill S pyogenes. Furthermore, S pyogenes grew more efficiently in HRG-deficient plasma, and clots formed in this plasma were significantly less effective at bacterial entrapment and killing. HRG-deficient mice were strikingly more susceptible to S pyogenes infection. These animals failed to control the infection at the local subcutaneous site, and abscess formation and inflammation were diminished compared with control animals. As a result, bacterial dissemination occurred more rapidly in HRG-deficient mice, and they died earlier and with a significantly higher mortality rate than control animals. HRG-deficient mice supplemented with purified HRG gave the same phenotype as control animals, demonstrating that the lack of HRG was responsible for the increased susceptibility. The results demonstrate a previously unappreciated role for HRG as a regulator of inflammation and in the defense at the local site of bacterial infection.
A single dose of a vesicular stomatitis virus-based influenza vaccine confers rapid protection against H5 viruses from different clades (2020)

The avian influenza virus outbreak in 1997 highlighted the potential of the highly pathogenic H5N1 virus to cause severe disease in humans. Therefore, effective vaccines against H5N1 viruses are needed to counter the potential threat of a global pandemic. We have previously developed a fast-acting and efficacious vaccine against Ebola virus (EBOV) using the vesicular stomatitis virus (VSV) platform. In this study, we generated recombinant VSV-based H5N1 influenza virus vectors to demonstrate the feasibility of this platform for a fast-acting pan-H5 influenza virus vaccine. We chose multiple approaches regarding antigen design and genome location to define a more optimized vaccine approach. After the VSV-based H5N1 influenza virus constructs were recovered and characterized in vitro, mice were vaccinated by a single dose or prime/boost regimen followed by challenge with a lethal dose of the homologous H5 clade 1 virus. We found that a single dose of VSV vectors expressing full-length hemagglutinin (HAfl) were sufficient to provide 100% protection. The vaccine vectors were fast-acting as demonstrated by uniform protection when administered 3 days prior to lethal challenge. Moreover, single vaccination induced cross-protective H5-specific antibodies and protected mice against lethal challenge with various H5 clade 2 viruses, highlighting the potential of the VSV-based HAfl as a pan-H5 influenza virus emergency vaccine.
Study of D J meson decays to D +π−, D 0π+ and D ∗+π− final states in pp collisions (2013)

A bstract A study of D + π − , D 0 π + and D ∗+ π − final states is performed using pp collision data, corresponding to an integrated luminosity of 1.0 fb −1 , collected at a centre-of-mass energy of 7 TeV with the LHCb detector. The D 1 (2420) 0 resonance is observed in the D ∗+ π − final state and the $ D_2^{*}\left( {2460} \right) $ resonance is observed in the D + π − , D 0 π + and D ∗+ π − final states. For both resonances, their properties and spin-parity assignments are obtained. In addition, two natural parity and two unnatural parity resonances are observed in the mass region between 2500 and 2800 MeV. Further structures in the region around 3000 MeV are observed in all the D ∗+ π − , D + π − and D 0 π + final states.
An examination of thermal modeling affects to the numerical prediction of large-scale cavitating fluid flow (2009)

The importance of modeling thermal effects in cavitatingfluid is examined in the context of computational fluid dynamics. Simulations of cavitation in water are used to study the effects of thermal versus and pressure variations in the fluid properties, and their impact on predictions. These studies are extended to evaluate energyconserving approaches compared to isothermal ones, to assess the underlying thermal models influence on the predicted cavities occurring in water. Results indicate that the thermal effects remain important, but only for specific applications that need high-frequency phenomena from the numerical simulation. Low-frequency measures, needed for loading analysis, appear to be relatively insensitive to thermal effects. Lastly, various thermally driven cavitation problems requiring energy-equation conservation are presented to display applications requiring such a formulation.
Faculty Opinions recommendation of Structural diversity in a human antibody germline library. (2019)

To support antibody therapeutic development, the crystal structures of a set of 16 germline variants composed of 4 different kappa light chains paired with 4 different heavy chains have been determined.All four heavy chains of the antigen-binding fragments (Fabs) have the same complementarity-determining region (CDR) H3 that was reported in an earlier Fab structure.The structure analyses include comparisons of the overall structures, canonical structures of the CDRs and the VH:VL packing interactions.The CDR conformations for the most part are tightly clustered, especially for the ones with shorter lengths.The longer CDRs with tandem glycines or serines have more conformational diversity than the others.CDR H3, despite having the same amino acid sequence, exhibits the largest conformational diversity.About half of the structures have CDR H3 conformations similar to that of the parent; the others diverge significantly.One conclusion is that the CDR H3 conformations are influenced by both their amino acid sequence and their structural environment determined by the heavy and light chain pairing.The stem regions of 14 of the variant pairs are in the 'kinked' conformation, and only 2 are in the extended conformation.The packing of the VH and VL domains is consistent with our knowledge of antibody structure, and the tilt angles between these domains cover a range of 11 degrees.Two of 16 structures showed particularly large variations in the tilt angles when compared with the other pairings.The structures and their analyses provide a rich foundation for future antibody modeling and engineering efforts.
Effectiveness of Purified Bacillus thuringiensis Berliner Insecticidal Proteins in Controlling Three Insect Pests of Australian Eucalypt Plantations (1996)

First instars of the chrysomelid leaf beetles Chrysophtharta bimaculata (Olivier) (Coleoptera: Chrysomelidae) and C. agricola (Chapuis) (Coleoptera: Chrysomelidae) were fed eucalypt leaves coated with purified protein encoded by the cryIIIA gene of Bacillus thuringiensis Berliner variety tenebrionis. The CryIIIA protein was highly effective against C. bimaculata when presented on adult Eucalyptus nitens (Deane & Maiden) Maiden leaves, but not when leaves of the preferred host E. regnans F. Muell. were used. The difference between tree species may be the result of differences in the amount of endotoxin ingested or to interactions between the cryIIIA protein and eucalypt allelochemicals, (e.g., leaf oils). CryIIIA was not effective against C. agricola fed on juvenile E. nitens leaves coated with cryIIIA protein. We also tested purified protein encoded by the cryIA(b) gene of B. thuringiensis variety kurstaki in laboratory feeding trials against 1st instars of Mnesampela Privata (Guené) (Lepidoptera: Ceometridae). The CryIA(b) protein was highly effective against M. privata when presented on juvenile E. mitens leaves. These results indicate that CryIIIA and CryIA(b) proteins could be used to control 1st instars of C. bimaculata and M. privata, respectively, in some commercial eucalypt plantations when either sprayed as microbial formulations or present in transgenic plants.
GaAs nanopillar-array solar cells employing in situ surface passivation (2013)

Arrays of III-V direct-bandgap semiconductor nanopillars represent promising photovoltaic candidates due to their inherent high optical absorption coefficients and minimized reflection arising from light trapping, efficient charge collection in the radial direction and the ability to synthesize them on low-cost platforms. However, the increased surface area results in surface states that hamper the power conversion efficiency. Here, we report the first demonstration of GaAs nanopillar-array photovoltaics employing epitaxial passivation with air mass 1.5 global power conversion efficiencies of 6.63%. High-bandgap epitaxial InGaP shells are grown in situ and cap the radial p-n junctions to alleviate surface-state effects. Under light, the photovoltaic devices exhibit open-circuit voltages of 0.44 V, short-circuit current densities of 24.3 mA cm(-2) and fill factors of 62% with high external quantum efficiencies >70% across the spectral regime of interest. A novel titanium/indium tin oxide annealed alloy is exploited as transparent ohmic anode.
Deposition of parallel arrays of palladium nanowires and electrical characterization using microelectrode contacts (2004)

In this work palladium nanowires have been synthesized by a template nanomanufacturing technique and their electrical properties have been studied. Freshly cleaved highly oriented pyrolytic graphite (HOPG) was exposed to PdCl2 solution and cyclic voltametry was used to obtain the deposition of the nanowires on the 'V' shaped grooves of HOPG. The morphology of the nanowires was analysed using an atomic force microscope (AFM) in non-contact mode and the diameter of the observed nanowires was measured to be approximately 250 nm. Scanning electron microscope (SEM) images revealed that the nanowires fabricated by this procedure were parallel and continuous. The deposited nanowires were transferred on a polystyrene film. The microelectrodes required to study the electrical properties have been patterned by silver evaporation and by shadow masking using a 60 µm tungsten wire. The electrical resistance measurements have shown that the array of nanowires is conducting and has resistance of the order of kilo-ohms.
Scalable Synthesis and Cancer Cell Cytotoxicity of Rooperol and Analogues (2022)

Plant polyphenols, such as the African potato (<i>Hypoxis hemerocallidea</i>)-derived bis-catechol rooperol, can display promising anticancer activity yet suffer from rapid metabolism. Embarking upon a program to systematically examine potentially more metabolically stable replacements for the catechol rings in rooperol, we report here a general, scalable synthesis of rooperol and analogues that builds on our previous synthetic approach incorporating a key Pd-catalyzed decarboxylative coupling strategy. Using this approach, we have prepared and evaluated the cancer cell cytotoxicity of rooperol and a series of analogues. While none of the analogues examined here were superior to rooperol in preventing the growth of cancer cells, analogues containing phenol or methylenedioxyphenyl replacements for one or both catechol rings were nearly as effective as rooperol.
A review of Modelling approaches for Ecological Risk Assessment of Pesticides (2022)

A wide diversity of plant protection products (PPP) is used for crop protection leading to the contamination of soil, water, and air, which can have ecotoxicological impacts on living organisms. It is inconceivable to study the effects of each compound on each species from each compartment, experimental studies being time consuming and cost prohibitive, and animal testing having to be avoided. Therefore, numerous models are developed to assess PPP ecotoxicological effects. Our objective was to provide an overview of the modeling approaches enabling the assessment of PPP effects (including biopesticides) on the biota. Six categories ofmodels were inventoried: (Q)SAR, DR and TKTD, population, multi-species, landscape, and mixture models. They were developed for various species (terrestrial and aquatic vertebrates and invertebrates, primary producers, micro-organisms) belonging to diverse environmental compartments, to address different goals (e.g., species sensitivity or PPP bioaccumulation assessment, ecosystem services protection). Among them, mechanistic models are increasingly recognized by EFSA for PPP regulatory risk assessment but, to date, remain not considered in notified guidance documents. The strengths and limits of the reviewed models are discussed together with improvement avenues (multigenerational effects, multiple biotic and abiotic stressors). This review also underlines a lack of model testing by means of field data and of sensitivity and uncertainty analyses. Accurate and robust modeling of PPP effects and other stressors on living organisms, from their application in the field to their functional consequences on the ecosystems at different scales of time and space, would help going toward a more sustainable management of the environment.
Coplanar-Gate Transparent Graphene Transistors and Inverters on Plastic (2012)

Transparent flexible graphene transistors and inverters in a coplanar-gate configuration were presented for the first time using only two materials: graphene and an ion gel gate dielectric. The novel device configuration simplifies device fabrication such that only two printing steps were required to fabricate transistors and inverters. The devices exhibited excellent device performances including low-voltage operation with a high transistor-on-current and mobility, excellent mechanical flexibility, environmental stability, and a reasonable inverting behavior upon connecting the two transistors.
Suppression of spin transport in ferromagnet/oxide/semiconductor junctions by magnetic impurities in the tunnel barrier (2016)

Abstract We have studied how the insertion of sub-monolayer amounts of Mn impurities in the middle of the oxide tunnel barrier of Fe/GeO 2 on p-type Ge affects the spin transport, using three-terminal Hanle measurements. Strikingly, the magnitude of the Hanle spin voltage is strongly reduced by increasing the amount of Mn dopants and is even completely absent for devices having an amount of Mn impurities equivalent to a 0.2-nm-thick layer. This demonstrates that magnetic impurities in the tunnel barrier are detrimental to the spin transport in ferromagnet/oxide/semiconductor junctions, and that the localized states associated with such magnetic impurities do not produce three-terminal Hanle spin signals.
A mild, efficient and catalyst-free thermoreversible ligation system based on dithiooxalates (2016)

We demonstrate a novel and ready to prepare thermoreversible hetero Diels-Alder dilinker on the basis of dithiooxalates, enabling the mild, rapid and catalyst-free linkage of diverse diene species under ambient conditions for applications in the fields of, for example, modular ligation, self-healing or recyclable materials and surface modification amongst others. The linker was studied using quantum chemical calculations, and experimentally in small molecular reactions via UV/Vis spectroscopy, mass spectrometry and NMR as well as in step-growth polymerizations with diene-difunctional building blocks-characterized via (temperature dependent) SEC and HT NMR-as an example for efficient polymer ligation. © The Royal Society of Chemistry 2016.
Limitations on Switching Speed in Wideband Semiconductor Lasers (2005)

An experimental and theoretical study of large-signal switching transients in directly modulated semiconductor lasers is reported. The main parameters affecting high-speed switching are identified and device-dependent limitations on modulation at multi-gigabit per second data rates are described.
A decade of plant proteomics and mass spectrometry: Translation of technical advancements to food security and safety issues (2013)

Tremendous progress in plant proteomics driven by mass spectrometry (MS) techniques has been made since 2000 when few proteomics reports were published and plant proteomics was in its infancy. These achievements include the refinement of existing techniques and the search for new techniques to address food security, safety, and health issues. It is projected that in 2050, the world's population will reach 9-12 billion people demanding a food production increase of 34-70% (FAO, 2009) from today's food production. Provision of food in a sustainable and environmentally committed manner for such a demand without threatening natural resources, requires that agricultural production increases significantly and that postharvest handling and food manufacturing systems become more efficient requiring lower energy expenditure, a decrease in postharvest losses, less waste generation and food with longer shelf life. There is also a need to look for alternative protein sources to animal based (i.e., plant based) to be able to fulfill the increase in protein demands by 2050. Thus, plant biology has a critical role to play as a science capable of addressing such challenges. In this review, we discuss proteomics especially MS, as a platform, being utilized in plant biology research for the past 10 years having the potential to expedite the process of understanding plant biology for human benefits. The increasing application of proteomics technologies in food security, analysis, and safety is emphasized in this review. But, we are aware that no unique approach/technology is capable to address the global food issues. Proteomics-generated information/resources must be integrated and correlated with other omics-based approaches, information, and conventional programs to ensure sufficient food and resources for human development now and in the future.
The absorption spectrum of formic-acid dimers in the vacuum ultraviolet region (1992)

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