gebhart/patrepeval huggingface dataset:
Selenite-induced Expression of a Caenorhabditis elegans Pro-aging Factor and Ortholog of Human Selenium-binding Protein 1 (2020)

Background: The essential trace element and micronutrient selenium exerts most of its biological actions through incorporation into selenoproteins as selenocysteine. Two further types of Se-containing proteins exist, including those that have selenomethionine incorporated instead of methionine, and the group of selenium-binding proteins. We previously described an ortholog of selenium-binding protein 1 (SELENBP1) in the nematode Caenorhabditis elegans, Y37A1B.5, and demonstrated that it confers resistance to toxic selenite concentrations while impairing general stress resistance and life expectancy of C. elegans. Objective: We tested for the effect of selenite on Y37A1B.5 expression, and we analyzed whether Y37A1B.5 also shows a lifespan-modulating effect when the nematodes are deficient in the selenoenzyme thioredoxin reductase-1 (TRXR-1). Methods: C. elegans expressing a translational reporter construct encoding GFP-tagged Y37A1B.5 under the control of the Y37A1B.5 promoter were exposed to selenite, followed by fluorescence microscopic analysis of GFP levels. Lifespan analyses and RNA interference experiments were performed in trxr-1-deficient worms. Results: We here demonstrate that selenite at toxic concentrations stimulates the expression of the translational Y37A1B.5 reporter. The lifespan-extending effect of Y37A1B.5 deficiency was preserved upon the deletion of the only selenoprotein in C. elegans, TRXR-1. Conclusion: These data suggest that (1) Y37A1B.5 may serve as a selenite-responsive buffer against high environmental selenium concentrations and that (2) lifespan extension elicited by Y37A1B.5 knockdown does not require functional TRXR-1.
Characterizing Practice Effects in Performance‐Based Tests Administered to Users of Unilateral Lower Limb Prostheses: A Preliminary Study (2020)

Results indicate that the FSST, TUG, and 10mWT were susceptible to practice effects in this sample of users of LLP. Practice effects may obscure significant differences in walking and balance ability, and thereby, in the absence of modifications, limit the use of these tests for making individual patient clinical decisions and analyzing group-level data.
Abdominal Aortic Aneurysm Is Associated with a Variant in Low-Density Lipoprotein Receptor-Related Protein 1 (2011)

Abdominal aortic aneurysm (AAA) is a common cause of morbidity and mortality and has a significant heritability. We carried out a genome-wide association discovery study of 1866 patients with AAA and 5435 controls and replication of promising signals (lead SNP with a p value < 1 × 10(-5)) in 2871 additional cases and 32,687 controls and performed further follow-up in 1491 AAA and 11,060 controls. In the discovery study, nine loci demonstrated association with AAA (p < 1 × 10(-5)). In the replication sample, the lead SNP at one of these loci, rs1466535, located within intron 1 of low-density-lipoprotein receptor-related protein 1 (LRP1) demonstrated significant association (p = 0.0042). We confirmed the association of rs1466535 and AAA in our follow-up study (p = 0.035). In a combined analysis (6228 AAA and 49182 controls), rs1466535 had a consistent effect size and direction in all sample sets (combined p = 4.52 × 10(-10), odds ratio 1.15 [1.10-1.21]). No associations were seen for either rs1466535 or the 12q13.3 locus in independent association studies of coronary artery disease, blood pressure, diabetes, or hyperlipidaemia, suggesting that this locus is specific to AAA. Gene-expression studies demonstrated a trend toward increased LRP1 expression for the rs1466535 CC genotype in arterial tissues; there was a significant (p = 0.029) 1.19-fold (1.04-1.36) increase in LRP1 expression in CC homozygotes compared to TT homozygotes in aortic adventitia. Functional studies demonstrated that rs1466535 might alter a SREBP-1 binding site and influence enhancer activity at the locus. In conclusion, this study has identified a biologically plausible genetic variant associated specifically with AAA, and we suggest that this variant has a possible functional role in LRP1 expression.
Time-domain electromagnetic simulation of hard surfaces (2002)

The concept of hard surfaces in electromagnetics is valuable tool for antenna design. In principle, hard horns and feeds can support a TEM mode with uniform field distribution and straight field lines over the aperture of an arbitrary cross-sectional shape. This makes them compact with high gain, and thus convenient to apply in cluster feeds and arrays (Kildal and Lier, 1990). Low cross-polarization is also expected. Hard surfaces are realised by dielectric-filled longitudinal corrugations or by longitudinal strips on a dielectric coating. Their potential has been previously been demonstrated experimentally. However, the theoretical relations given so far apply only to a limited class of hard structures. They are typically derived assuming that the period of corrugations is small compared to the wavelength and that the corrugations are rectangular and much wider than the edges between them (Kildal, 1990). The aim of the present paper is to show that the finite-difference time-domain method applied to hard surfaces provides deeper insight into their behaviour, and also permits fast evaluation of their characteristics.
Web Traffic Analysis Introduction (2002)

Knowing how much traffic your site gets, and which parts get more than others, can be vitally important to understanding what visitors are getting from your site. With a bit of analysis of your server logs, you could discover that people are using your site in a way you didn't expect. Once you get a better idea of usage patterns for your site, you can begin to work on ironing out any problem areas such as that page that most shoppers never get beyond.KeywordsUsage PatternSite TrafficPage RequestAuthor InterviewBusiness TeamThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Effects of Indole Derivatives on the Growth of Chlamydomonas spp. as Causal Green Algae in the Hydroponics System. (2001)

Some Indole derivatives were examined for their algicidal activity to Chlamydomonas reinhardtii and the isolate of Chlamydomonas sp. which was isolated from the green algae-generating hydroponic solution in the hydroponic trough of the greenhouse. Of nine compounds tested, 3-indolepropionic acid, 3-indoleacrylic acid and 3- (3-indolyl) butanoic acid could suppress multiplication of C. reinhardtii cells. Although the first one exhibited the strong phytotoxicity, the latter two compounds were effective at lower concentrations which did not cause any toxic effect on the growth of tomato seedlings. In addition, 3-indoleacrylic acid and 3- (3-indolyl) butanoic acid inhibited the multiplication of Chlamydomonas green algae which were actively growing in our hydroponic facilities, and suppressed the algal emergence on the synthetic sponge cubes soaked in the hydroponic solution which had been inoculated with green algae. The present study suggests that these compounds have a potential to be used as an algicide for the hydroponics of crop plants.
Requirements for control of B‐cell lymphoma by NK cells (2009)

The role of NK cells in the control of endogenously arising tumors is still unclear. We monitored activation and effector functions of NK cells in a c-myc-transgenic mouse model of spontaneously arising lymphoma. At early stages, tumors demonstrated reduced MHC class I expression and increased expression of natural killer group 2D ligands (NKG2D-L). NK cells in these tumors showed an activated phenotype that correlated with the loss of tumor MHC class I. With increasing tumor load however, NK-cell effector functions became progressively paralyzed or exhausted. In later stages of disease, tumors re-expressed MHC class I and lost NKG2D-L, suggesting a role of these two signals for NK cell-mediated tumor control. Testing a panel of lymphoma cell lines expressing various MHC class I and NKG2D-L levels suggested that NK cell-dependent tumor control required a priming and a triggering signal that were provided by MHC class I down-regulation and by NKG2D-L, respectively. Deleting either of the "two signals" resulted in tumor escape. At early disease stages, immune stimulation through TLR-ligands in vivo efficiently delayed lymphoma growth in a strictly NK cell-dependent manner. Thus, NK-receptor coengagement is crucial for NK-cell functions in vivo and especially for NK cell-mediated tumor surveillance.
DCT approximation for low bit rate coding using a conditional transform (2003)

A transform approximation is explored for speeding up the software compression of images and video. It is used to replace the regular DCT whenever only a few DCT coefficients are actually encoded. A conditional transform is proposed to transform only the data that it determines to be relevant. The approximation is applicable to environments combining the requirements of high compression and low complexity.
An electro-optic modulator assisted by nonlinear coupling dynamics (2003)

Summary form only given. We propose using the bistability of a nonlinear coupler not for an all-optical switch, but to enhance the sensitivity of an electrically-controlled modulator. This is potentially of enormous benefit. Essentially all performance limitations of electro-optic modulators are related to the trade-offs in achieving a /spl pi/ phase shift with a low drive voltage. By reducing the phase shift required for full switching, our scheme would loosen this fundamental constraint and allow higher bandwidth, lower power consumption, etc. We present simulations demonstrating the basic principle of the Kerr-assisted modulator, and calculate the moderate performance degradation due to optical power fluctuations.
Soft Matters with Electronic Functions ^|^mdash;Development to Soft Electronic Systems (2011)

のを きっかけとして (1) ，芳香族化合物の分子性結晶の電子機能が
High Efficiency Gene Transfer to Human Hematopoietic SCID-Repopulating Cells Under Serum-Free Conditions (1998)

Stable gene transfer to human pluripotent hematopoietic stem cells (PHSCs) is an attractive strategy for the curative treatment of many genetic hematologic disorders. In clinical trials, the levels of gene transfer to this cell population have generally been low, reflecting deficiencies in both the vector systems and transduction conditions. In this study, we have used a pseudotyped murine retroviral vector to transduce human CD34(+) cells purified from bone marrow (BM) and umbilical cord blood (CB) under optimized conditions. After transduction, 71% to 97% of the hematopoietic cells were found to express a low-affinity nerve growth factor receptor (LNGFR) marker gene. Six weeks after transplantation into immunodeficient NOD/LtSz-scid/scid (NOD/SCID) mice, LNGFR expression was detected in 6% to 57% of CD45(+) cells in eight of nine engrafted animals. Moreover, proviral DNA was detected in 8.3% to 45% of secondary colonies derived from BM cells of engrafted NOD/SCID mice. Our data show consistent transduction of SCID-repopulating cells (SRCs) and suggest that the efficiency of gene transfer to human hematopoietic repopulating cells can be improved using existing retroviral vector systems and carefully optimized transduction conditions.
BODIPY–Tetrazine Derivatives as Superbright Bioorthogonal Turn‐on Probes (2013)

Am schnellsten und hellsten: Die enge Verknüpfung von Tetrazin an ein BODIPY-Fluorophor führt zu einem außergewöhnlich effizienten Energietransfer und starker Fluoreszenzlöschung. Nach Reaktion des Tetrazins nimmt die Fluoreszenzintensität um mehr als das Tausendfache zu. Damit ist die fluorogene Aktivierung um zwei Größenordnungen höher als zuvor beschrieben. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Application response measurement of distributed web services (2002)

Web service technology will provide a platform for dynamic e-business applications. This paper describes a framework for identifying, monitoring and reporting performance data of critical transactions within a web service using the Java ARM standard, a Transaction Definition Language (TDL) and a bytecode instrumentation tool. The data extracted using this framework is shown to be appropriate for dynamically selecting web services based on performance metrics. A case study is provided using Gourmet2Go, an IBM demonstrator of a dynamic business-to-business (B2B) application.
Near field imaging with resonant cavity lens (2010)

We showed that a Ag-SiO(2)-Ag Fabry-Pérot cavity can be used in near-field imaging based on omnidirectional resonance tunneling. The omnidirectional resonance was experimentally demonstrated in the Ag-SiO(2)-Ag resonant cavity working at a wavelength of 365 nm. The resonant cavity lens with high transmittance and high image fidelity was fabricated using standard photolithography method. Grating source with 190 nm line resolution was imaged through the resonant cavity lens with a total thickness of 128 nm.
Stochastic Expectation Maximization Algorithm for Long-Memory Fast-Fading Channels (2010)

In this paper, we develop a novel statistical detection algorithm following similar principles to that of expectation maximization (EM) algorithm. Our goal is to develop an iterative algorithm for joint channel estimation and data detection in channels that have a long memory and are fast varying in time. At each iteration, starting with an estimate of the channel, we combine a Markov Chain Monte Carlo (MCMC) algorithm for data detection, and an adaptive algorithm for channel tracking, to develop a statistical search procedure that finds joint important samples of possible transmitted data and channel impulse responses. The result of this step, which may be thought as E-step of the proposed algorithm, is used in an M-step that refines the channel estimate, for the next iteration. Excellent behavior of the proposed algorithm is presented by examining it on real data from underwater acoustic communication channels.
Smart Strategies for Precise Delivery of CRISPR/Cas9 in Genome Editing (2022)

The emergence of CRISPR/Cas technology has enabled scientists to precisely edit genomic DNA sequences. This approach can be used to modulate gene expression for the treatment of genetic disorders and incurable diseases such as cancer. This potent genome-editing tool is based on a single guide RNA (sgRNA) strand that recognizes the targeted DNA, plus a Cas nuclease protein for binding and processing the target. CRISPR/Cas has great potential for editing many genes in different types of cells and organisms both <i>in vitro</i> and <i>in vivo</i>. Despite these remarkable advances, the risk of off-target effects has hindered the translation of CRISPR/Cas technology into clinical applications. To overcome this hurdle, researchers have devised gene regulatory systems that can be controlled in a spatiotemporal manner, by designing special sgRNA, Cas, and CRISPR/Cas delivery vehicles that are responsive to different stimuli, such as temperature, light, magnetic fields, ultrasound (US), pH, redox, and enzymatic activity. These systems can even respond to dual or multiple stimuli simultaneously, thereby providing superior spatial and temporal control over CRISPR/Cas gene editing. Herein, we summarize the latest advances on smart sgRNA, Cas, and CRISPR/Cas nanocarriers, categorized according to their stimulus type (physical, chemical, or biological).
Vaccination: Past, Present and Future (2003)

Introduction Vaccination: The Past Vaccination: The Present Vaccination: The Future Conclusion: The Intangible Value of Vaccination References
Interaction Trap/Two‐Hybrid System to Identify Interacting Proteins (1999)

The parameters and experimental conditions for this important system are constantly undergoing improvement. This newest version includes expanded tables describing interaction trap components and additional libraries compatible with the interaction trap system. It also features a new protocol on performing a hunt by interaction mating. Some of the commercial vendors selling yeast two-hybrid reagents recommend using interaction mating to perform a hunt, so this procedure should be of great interest.
Discovery of Inducible Nitric Oxide Synthase (iNOS) Inhibitor Development Candidate KD7332, Part 1: Identification of a Novel, Potent, and Selective Series of Quinolinone iNOS Dimerization Inhibitors that are Orally Active in Rodent Pain Models (2009)

There are three isoforms of dimeric nitric oxide synthases (NOS) that convert arginine to citrulline and nitric oxide. Inducible NOS is implicated in numerous inflammatory diseases and, more recently, in neuropathic pain states. The majority of existing NOS inhibitors are either based on the structure of arginine or are substrate competitive. We describe the identification from an ultra high-throughput screen of a novel series of quinolinone small molecule, nonarginine iNOS dimerization inhibitors. SAR studies on the screening hit, coupled with an in vivo lipopolysaccharide (LPS) challenge assay measuring plasma nitrates and drug levels, rapidly led to the identification of compounds 12 and 42--potent inhibitors of the human and mouse iNOS enzyme that were highly selective over endothelial NOS (eNOS). Following oral dosing, compounds 12 and 42 gave a statistical reduction in pain behaviors in the mouse formalin model, while 12 also statistically reduced neuropathic pain behaviors in the chronic constriction injury (Bennett) model.
Data from Human Papillomavirus 42 Drives Digital Papillary Adenocarcinoma and Elicits a Germ Cell–like Program Conserved in HPV-Positive Cancers (2023)

&lt;div&gt;Abstract&lt;p&gt;The skin is exposed to viral pathogens, but whether they contribute to the oncogenesis of skin cancers has not been systematically explored. Here we investigated 19 skin tumor types by analyzing off-target reads from commonly available next-generation sequencing data for viral pathogens. We identified human papillomavirus 42 (HPV42) in 96% (&lt;i&gt;n&lt;/i&gt; = 45/47) of digital papillary adenocarcinoma (DPA), an aggressive cancer occurring on the fingers and toes. We show that HPV42, so far considered a nononcogenic, “low-risk” HPV, recapitulates the molecular hallmarks of oncogenic, “high-risk” HPVs. Using machine learning, we find that HPV-driven transformation elicits a germ cell–like transcriptional program conserved throughout all HPV-driven cancers (DPA, cervical carcinoma, and head and neck cancer). We further show that this germ cell–like transcriptional program, even when reduced to the top two genes (&lt;i&gt;CDKN2A&lt;/i&gt; and &lt;i&gt;SYCP2&lt;/i&gt;), serves as a fingerprint of oncogenic HPVs with implications for early detection, diagnosis, and therapy of all HPV-driven cancers.&lt;/p&gt;Significance:&lt;p&gt;We identify HPV42 as a uniform driver of DPA and add a new member to the short list of tumorigenic viruses in humans. We discover that all oncogenic HPVs evoke a germ cell–like transcriptional program with important implications for detecting, diagnosing, and treating all HPV-driven cancers.&lt;/p&gt;&lt;p&gt;&lt;i&gt;&lt;a href="https://aacrjournals.org/cancerdiscovery/article/doi/10.1158/2159-8290.CD-22-1150" target="_blank"&gt;See related commentary by Starrett et al., p. 17&lt;/a&gt;.&lt;/i&gt;&lt;/p&gt;&lt;p&gt;&lt;i&gt;&lt;a href="https://aacrjournals.org/cancerdiscovery/article/doi/10.1158/2159-8290.CD-13-1-ITI" target="_blank"&gt;This article is highlighted in the In This Issue feature, p. 1&lt;/a&gt;&lt;/i&gt;&lt;/p&gt;&lt;/div&gt;
Edge analytics as service &#x2014; A service oriented framework for real time and personalised recommendation analytics (2013)

Due to the advent of technology and internet over the past few years, significant number of customers have started shopping online and accessing their bank account through various channels like Netbanking, Mobile banking etc. In this paper, we describe Edge Analytics framework which deliver analytics as a service that can be hosted by a financial institute like Bank for delivering personalized offer in real time on thier netbanking portals or on other channels. This edge analytics service can be accessed through edge APIs plugged into netbanking portals. It access the recent transactions in the log to determine offers for the customer and therefore, saving a lot of resources and effort by fetching the data from the main warehouse. Edge analytics server capability has been enhanced by incorprating knowledge such as user's intent and interest from their social media profile by identifying their identity on the Online social network. This information is then feed into rule engine to generate customised offers for each user using both enterprise and social information. Edge Analytics service has been hosted on cloud which renders it scalability factor and allows different third party provders to make use of the service easily..
Corticotropinoma as a Component of Carney Complex (2017)

Known germline gene abnormalities cause one-fifth of the pituitary adenomas in children and adolescents, but, in contrast with other pituitary tumor types, the genetic causes of corticotropinomas are largely unknown. In this study, we report a case of Cushing disease (CD) due to a loss-of-function mutation in <i>PRKAR1A</i>, providing evidence for association of this gene with a corticotropinoma. A 15-year-old male presenting with hypercortisolemia was diagnosed with CD. Remission was achieved after surgical resection of a corticotropin (ACTH)-producing pituitary microadenoma, but recurrence 3 years later prompted reoperation and radiotherapy. Five years after the original diagnosis, the patient developed ACTH-independent Cushing syndrome, and a diagnosis of primary pigmented nodular adrenocortical disease was confirmed. A <i>PRKAR1A</i> mutation (c.671delG, p.G225Afs*16) was detected in a germline DNA sample from the patient, which displayed loss of heterozygosity in the corticotropinoma. No other germline or somatic mutations of interest were found. As corticotropinomas are not a known component of Carney complex (CNC), we performed loss of heterozygosity and messenger RNA stability studies in the patient's tissues, and analyzed the effect of <i>Prkar1a</i> silencing on AtT-20/D16v-F2 mouse corticotropinoma cells. No <i>PRKAR1A</i> defects were found among 97 other pediatric CD patients studied. Our clinical case and experimental data support a role for <i>PRKAR1A</i> in the pathogenesis of a corticotroph cell tumor. This is a molecularly confirmed report of a corticotropinoma presenting in association with CNC. We conclude that germline <i>PRKAR1A</i> mutations are a novel, albeit apparently infrequent, cause of CD.