gebhart/patrepeval huggingface dataset:
An optimal harvest date prediction tool for long-term storage of red currants (2013)

Harvesting at the optimal time is critical for a high quality product destined for long-term storage. Still, there are insufficient tools to determine optimal harvest date. In this work, we present a very practical optimal harvest date tool based on a model developed several years ago and tested with several crops such as cherries, peaches, and nectarines. We have developed and validated the model with data collected for red-currants from the seasons 2005 to 2010 and found to be a useful and quite precise tool for optimal harvest date of red currants destined to long-term storage. This model is based on the concept that fruit developmental rates are primarily governed by heat exposure during the first 30 days after bloom. This study provides practical evidence of the suitability of this tool for growers for harvesting at the right time allowing them to plan in advance the harvest season. It might potentially be implemented for many other crops.
Blood ACE Phenotyping for Personalized Medicine: Revelation of Patients with Conformationally Altered ACE (2023)

<b>Background</b>: The angiotensin-converting enzyme (ACE) metabolizes a number of important peptides participating in blood pressure regulation and vascular remodeling. Elevated blood ACE is a marker for granulomatous diseases and elevated ACE expression in tissues is associated with increased risk of cardiovascular diseases. <b>Objective and Methodology:</b> We applied a novel approach -ACE phenotyping-to find a reason for conformationally impaired ACE in the blood of one particular donor. Similar conformationally altered ACEs were detected previously in 2-4% of the healthy population and in up to 20% of patients with uremia, and were characterized by significant increase in the rate of angiotensin I hydrolysis. <b>Principal findings:</b> This donor has (1) significantly increased level of endogenous ACE inhibitor in plasma with MW less than 1000; (2) increased activity toward angiotensin I; (3) M71V mutation in <i>ABCG2</i> (membrane transporter for more than 200 compounds, including bilirubin). We hypothesize that this patient may also have the decreased level of free bilirubin in plasma, which normally binds to the N domain of ACE. Analysis of the local conformation of ACE in plasma of patients with Gilbert and Crigler-Najjar syndromes allowed us to speculate that binding of mAbs 1G12 and 6A12 to plasma ACE could be a natural sensor for estimation of free bilirubin level in plasma. Totally, 235 human plasma/sera samples were screened for conformational changes in soluble ACE. <b>Conclusions/Significance:</b> ACE phenotyping of plasma samples allows us to identify individuals with conformationally altered ACE. This type of screening has clinical significance because this conformationally altered ACE could not only result in the enhancement of the level of angiotensin II but could also serve as an indicator of free bilirubin levels.
Effective nerve cell modulation by electrical stimulation of carbon nanotube embedded conductive polymeric scaffolds (2018)

Biomimetic biomaterials require good biocompatibility and bioactivity to serve as appropriate scaffolds for tissue engineering applications. Recent developments demonstrated that the unique properties of carbon nanotubes (CNTs) can enhance neural cell growth and axon organization. We previously developed a promising nerve conduit manufactured from biodegradable polycaprolactone fumarate (PCLF) for use in peripheral nerve regeneration applications. In the present study, we fabricated conductive PCLF-CNT scaffolds using ultraviolet (UV) induced photocrosslinking. We confirmed the successful incorporation of CNTs into the PCLF-CNT scaffolds which exhibited improved surface roughness compared with plain PCLF by scanning electronic microscopy, transmission electronic microscopy, and atomic force microscopy examinations. The PCLF-CNT substrates also had reduced impedance by electrochemical measurements. Enhanced PC-12 cell growth and differentiation were observed on PCLF-CNT sheets compared with PCLF sheets, indicating the beneficial effects of embedding CNTs into PCLF. Electrical stimulation not only enhanced PC-12 cell proliferation and neurite extension, but also promoted cellular migration and intracellular connections, which are all critical cellular behaviours for nerve regeneration. Overall, this study provides a new promising strategy for using electrically conductive PCLF-CNT nerve scaffolds in regenerative medicine.
WSG: Challenges for future RF integration (2009)

Current and future transceivers for mobile terminals are facing increasing complexity because of the market's demands for lower cost, lower power consumption, and higher data-rate. As a result, multi-band and multi-mode radios covering the diversity of communication standards impart unique challenges on the RF-transceiver design due to limitations in terms of reconfigurable RF components that meet the demanding cellular performance criteria at costs that are attractive for mass market applications. The focus of this workshop will be on the challenges the cellular standards pose on future transceiver integration, along with a thorough discussion of advanced techniques for receivers and transmitters towards integration in SoC approaches including novel architectures, highly configurable analog circuitry, digitally assisted and enhanced analog/RF modules, and the integration of digital signal processing into the traditionally purely analog front-end. However, the utilization of digital signal processing capabilities is in line with the ongoing trend towards SoCs in minimum-feature-sized CMOS in the cellular market. Moreover, advances in the field of RF front-end modules and novel analog signal processing architectures are covered to give a consolidated outlook on future concepts for cellular radios.
The potential gradient field created by epicardial defibrillation electrodes in dogs. (1986)

Knowledge of the potential gradient field created by defibrillation electrodes is important for the understanding and improvement of defibrillation. To obtain this knowledge by direct measurements, potentials were recorded from 60 epicardial, eight septal, and 36 right ventricular transmural electrodes in six open-chest dogs while 1 to 2 V shocks were given through defibrillation electrodes on the right atrium and left ventricular apex (RA. V) and on the right and left ventricles (RV .LV). The potential gradient field across the ventricles was calculated for these low voltages. Ventricular fibrillation was electrically induced, and ventricular activation patterns were recorded after delivering high-voltage shocks just below the defibrillation threshold. With the low-voltage shocks, the potential gradient field was very uneven, with the highest gradient near the epicardial defibrillation electrodes and the weakest gradient distant from the defibrillation electrodes for both RA. V and RV .LV combinations. The mean ratio of the highest to the lowest measured gradient over the entire ventricular epicardium was 19.4 +/- 8.1 SD for the RA. V combination and 14.4 +/- 3.4 for the RV .LV combination. For both defibrillation electrode combinations, the earliest sites of activation after unsuccessful shocks just below the defibrillation threshold were located in areas where the potential gradient was weak for the low-voltage shocks. We conclude that there is a markedly uneven distribution of potential gradients for epicardial defibrillation electrodes with most of the voltage drop occurring near the electrodes, the potential gradient field is significant because it determines where shocks fail to halt fibrillation, and determination of the potential gradient field should lead to the development of improved electrode locations for defibrillation.
Explosive Detection by Fluorescent Electrospun Polymer Membrane Sensor (2004)

Introduction Chemical sensors for explosive detection have attracted increasing attention recendy due to heightened awareness of terrorist and criminal activities. Numerous methods of direct explosive detection, including ion mobility spectrometry, neutron analysis, X-ray backscattering, and electron capture detection have been developed. However, there remains urgent need for new approaches that not only complement existing methods, but improve on them in terms of lower cost and greater instrumental simplicity (1). Fluorescent optical chemical sensors are of particular interest due to their inherent sensitivity and simplicity (2). These types of sensors have many other advantages that optical sensors, in general, offer. One of the most attractive features is that they do not require a separate reference sensor, as a potentiometric chemical sensor does. In addition, they are not affected by electrical interference, sample flow rate, and stir speed which can be serious problems with electrochemical sensors. Fluorescent optical chemical sensors have been
Practical Synthesis of BILA 2157 BS, a Potent and Orally Active Renin Inhibitor:  Use of an Enzyme-Catalyzed Hydrolysis for the Preparation of Homochiral Succinic Acid Derivatives (1999)

We have developed a highly convergent and stereoselective synthesis of BILA 2157 BS, a potent and orally active renin inhibitor. The synthesis proceeds in 15 distinct chemical steps (with several integrated, multistep operations) from aminodiol 4. The key step in the synthesis involves the use of an enantiospecific, enzyme-catalyzed hydrolysis of a substituted succinate diester to provide a homochiral succinic acid derivative in 98% enantiomeric excess (>/=2.5 kg scale). Recycling of the unwanted enantiomer is accomplished through base-catalyzed racemization, leading to an efficient deracemization of the starting racemic diester. The entire sequence proceeds without chromatographic purifications and delivers the product with >97% homogeneity. In addition, compared to the previously reported syntheses of BILA 2157 BS, this approach avoids the use of expensive chiral auxiliaries and cryogenics and, thus, should be amenable to the preparation of large quantities of this peptidomimetic inhibitor.
High Efficiency of a Sequential Recombinase-Mediated Cassette Exchange Reaction in <i>Escherichia coli</i> (2010)

A comparison between the efficiency of recombinase-mediated cassette exchange (RMCE) reactions catalyzed in Escherichia coli by the site-specific recombinases Flp of yeast and Int of coliphage HK022 has revealed that an Flp-catalyzed RMCE reaction is more efficient than an Int-HK022 catalyzed reaction. In contrast, an RMCE reaction with 1 pair of frt sites and 1 pair of att sites catalyzed in the presence of both recombinases is very inefficient. However, the same reaction catalyzed by each recombinase individually supplied in a sequential order is very efficient, regardless of the order. Atomic force microscopy images of Flp with its DNA substrates show that only 1 pair of recombination sites forms a synaptic complex with the recombinase. The results suggest that the RMCE reaction is sequential.
Modular Hybrid Autoregressive Transducer (2023)

Text-only adaptation of a transducer model remains challenging for end-to-end speech recognition since the transducer has no clearly separated acoustic model (AM), language model (LM) or blank model. In this work, we propose a modular hybrid autoregressive transducer (MHAT) that has structurally separated label and blank decoders to predict label and blank distributions, respectively, along with a shared acoustic encoder. The encoder and label decoder outputs are directly projected to AM and internal LM scores and then added to compute label posteriors. We train MHAT with an internal LM loss and a HAT loss to ensure that its internal LM becomes a standalone neural LM that can be effectively adapted to text. Moreover, text adaptation of MHAT fosters a much better LM fusion than internal LM subtraction-based methods. On Google's large-scale production data, a multi-domain MHAT adapted with 100B sentences achieves relative WER reductions of up to 12.4% without LM fusion and 21.5% with LM fusion from 400K-hour trained HAT.
Local electrostatic effects of surface structure on field emission (2002)

We examined the classical electrostatic effects due to geometric surface structures on conductive field emission needles numerically using the finite element method and compared our results to several commonly applied analytic relations. Analysis of the morphology of electrochemically prepared Mo needles by high-resolution transmission electron microscopy was incorporated in the numerical analysis in the form of small surface protrusions and gross needle shape. We found that the error between the electrostatic potential defined by popular analytic equations and both analytic equations derived in prolate spheroidal coordinates and finite element method results was significant for ellipsoidal needles with and without surface protrusions. The morphology of the surface protrusion was found to introduce a significant nonlinear potential barrier near the needle surface. Finally we numerically analyzed a nonsymmetric, nonhomogeneous experimental needle indicating that even larger errors in the electrostatic potential can be expected relative to analytic equations.
Targeting KRAS mutations with HLA class II-restricted TCRs for the treatment of solid tumors (2021)

T-cell receptor (TCR) redirected T cells are considered as the next generation of care for the treatment of numerous solid tumors. KRAS mutations are driver neoantigens that are expressed in over 25% of all cancers and are thus regarded as ideal targets for Adoptive Cell Therapy (ACT). We have isolated four KRAS-specific TCRs from a long-term surviving pancreatic cancer patient vaccinated with a mix of mutated KRAS peptides. The sequence of these TCRs could be identified and expressed in primary cells. We demonstrated stable expression of all TCRs as well as target-specific functionality when expressing T cells were co-incubated with target cells presenting KRAS peptides. In addition, these TCRs were all partially co-receptor independent since they were functional in both CD4 and CD8 T cells, thus indicating high affinity. Interestingly, we observed that certain TCRs were able to recognize several KRAS mutations in complex with their cognate Human leukocyte antigen (HLA), suggesting that, here, the point mutations were less important for the HLA binding and TCR recognition, whereas others were single-mutation restricted. Finally, we demonstrated that these peptides were indeed processed and presented, since HLA-matched antigen presenting cells exogenously loaded with KRAS proteins were recognized by TCR-transduced T cells. Taken together, our data demonstrate that KRAS mutations are immunogenic for CD4 T cells and are interesting targets for TCR-based cancer immunotherapy.
Method and Apparatus for the Automated Delivery of Continuous Neural Stem Cell Trails Into the Spinal Cord of Small and Large Animals (2018)

The TrailmakerTM creates longitudinal cellular trails spanning multiple levels from a single-entry point. This may enhance the ability of therapeutics to promote functional relays after SCI.
MEASURING CHARGE AND CHARGE DECAY IN FLOATING-ELECTRODE ELECTROSTATIC ACTUATORS (2008)

We consider electrostatic actuators that are pre-energized with charge, which amplifies their response to input voltages.Floatingelectrode actuators were designed, fabricated, and characterized.A non-disruptive measurement of charge is proposed and demonstrated.When the floating electrode is charged, the system has two distinct pull-in voltages.It is shown that the amplitude of charge is proportional to the average of the two pull-in voltages.In this work, we present the correct way of using energy methods to analyze systems that are loaded by both charge and voltage.
Two New Pseudopod Morphologies Displayed by the Human Hematopoietic KG1a Progenitor Cell Line and by Primary Human CD34+Cells (1998)

A primitive human hematopoietic myeloid progenitor cell line, KG1a, characterized by high expression of the CD34 surface antigen has been observed to extend long, thin pseudopodia. Once extended, these pseudopods may take on one of two newly described morphologies, tenupodia or magnupodia. Tenupodia are very thin and form in linear segments. They adhere to the substrate, can bifurcate multiple times, and often appear to connect the membranes of cells more than 300 micrometer apart. Magnupodia are much thicker and have been observed to extend more than 330 micrometer away from the cell. Magnupods are flexible and can exhibit rapid dynamic motion, extending or retracting in a few seconds. During retraction, the extended material often pools into a bulb located on the pod. Both morphologies can adhere to substrates coated with fibronectin, collagen IV, and laminin as well as plastic. The CD34 and CD44 antigens are also present on the surface of these podia. Primary human CD34(+) cells from fetal liver, umbilical cord blood, adult bone marrow, and mobilized peripheral blood extend these podia as well. The morphology that these pseudopods exhibit suggest that they may play both sensory and mechanical roles during cell migration and homing after bone marrow transplantation.
The von Hippel-Lindau Tumor Suppressor Protein and Egl-9-Type Proline Hydroxylases Regulate the Large Subunit of RNA Polymerase II in Response to Oxidative Stress (2008)

Human renal clear cell carcinoma (RCC) is frequently associated with loss of the von Hippel-Lindau (VHL) tumor suppressor (pVHL), which inhibits ubiquitylation and degradation of the alpha subunits of hypoxia-inducible transcription factor. pVHL also ubiquitylates the large subunit of RNA polymerase II, Rpb1, phosphorylated on serine 5 (Ser5) within the C-terminal domain (CTD). A hydroxylated proline 1465 within an LXXLAP motif located N-terminal to the CTD allows the interaction of Rpb1 with pVHL. Here we report that in RCC cells, pVHL regulates expression of Rpb1 and is necessary for low-grade oxidative-stress-induced recruitment of Rpb1 to the DNA-engaged fraction and for its P1465 hydroxylation, phosphorylation, and nondegradative ubiquitylation. Egln-9-type prolyl hydroxylases, PHD1 and PHD2, coimmunoprecipitated with Rpb1 in the chromatin fraction of VHL(+) RCC cells in response to oxidative stress, and PHD1 was necessary for P1465 hydroxylation while PHD2 had an inhibitory effect. P1465 hydroxylation was required for oxidative-stress-induced Ser5 phosphorylation of Rpb1. Importantly, overexpression of wild-type Rpb1 stimulated formation of kidney tumors by VHL(+) cells, and this effect was abolished by P1465A mutation of Rpb1. These data indicate that through this novel pathway involving P1465 hydroxylation and Ser5 phosphorylation of Rbp1, pVHL may regulate tumor growth.
The Study of Pilot Allocation and Channel Estimation in Downlink OFDMA Systems (2007)

The OFDMA technology has been proposed as one of the main downlink schemes for the B3G systems. In this paper, the characteristics of a Wiener filter based channel estimation scheme have been investigated for the downlink OFDMA system. The basic pilot patterns have also been studied for the proposed channel estimator. In general, it has been shown that with proper pilot density, a 2D Wiener filter can provide good performance in different channel conditions. So such a channel estimator can be proposed as one of the suitable channel estimation schemes for the downlink OFDMA system.
Shining Light on the Dark Side of Imaging: Excited State Absorption Enhancement of a Bis-styryl BODIPY Photoacoustic Contrast Agent (2014)

A first approach toward understanding the targeted design of molecular photoacoustic contrast agents (MPACs) is presented. Optical and photoacoustic Z-scan spectroscopy was used to identify how nonlinear (excited-state) absorption contributes to enhancing the photoacoustic emission of the curcuminBF2 and bis-styryl (MeOPh)2BODIPY dyes relative to Cy3.
Mexique insurge (2010)

Une bibliothÃ¨que numÃ©rique unique et originale dans le monde francophone en sciences sociales et humaines, dÃ©veloppÃ©e en collaboration avec l'UniversitÃ© du QuÃ©bec Ã Chicoutimi, fondÃ©e et dirigÃ©e par Jean-Marie Tremblay, bÃ©nÃ©vole, professeur de sociologie au dÃ©partement des sciences humaines du CÃ©gep de Chicoutimi.
Metabolic profiling of Solanum villosum Mill subsp. miniatum (bernh. ex willd.): Hepatoprotective and antifibrotic activity in a rat model of liver fibrosis (2019)

Pharmacognosy Magazine ,2019,15,65,659-670.DOI:10.4103/pm.pm_547_18Published:September 2019Type:Original Article Authors:Alaa El-Din E. Abdel-Hamid, Riham Salah El Dine, Jandirk Sendker, Soheir M El Zalabani, Meselhy R Meselhy, Elena Jimenez-Negro, and Ashraf B Abdel-Naim Author(s) affiliations:Alaa El-Din E. Abdel-Hamid1, Riham Salah El Dine1, Jandirk Sendker2, Soheir M El Zalabani1, Meselhy R Meselhy1, Elena Jimenez-Negro2, Ashraf B Abdel-Naim3 1Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt 2Institute of Pharmaceutical Biology and Phytochemistry, University of Münster, Münster, Germany 3Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia Abstract:Aim/Background: To assess the liver antifibrotic action of ethanolic extract of aerial parts of Solanum villosum Mill subsp. miniatum (Bernh. ex Willd.) (SVE) and correlate this activity with its high performance liquid chromatography-quadrupole time of flight (HPLC-qTOF) Electrospray Ionisation Mass Spectrometry (ESIMS) phytochemical profile. Materials and Methods: The median lethal dose of SVE was determined, and a rat model of carbon tetrachloride (CCl4)-induced liver fibrosis was used to evaluate its antifibrotic activity. Markers for hepatotoxicity, fibrosis, and oxidative stress were assessed, and histopathological features of liver tissues were examined. Metabolite profiling of SVE was achieved via HPLC-qTOF-ESIMS coupled with Photodiode array (PDA). Liver fibrosis was induced in rats by oral administration of CCl4for 6 weeks. Silymarin (positive control) and SVE (100 and 250 mg/kg) were orally administered daily for 6 weeks. Results: Compared to CCl4-intoxicated group, administration of SVE obviously ameliorated fibrosis of the hepatic capsule associated with aggregation of multiple focal fat cells formation. Both silymarin and SVE ameliorated the rise in serum markers of hepatotoxicity (alanine transaminase, aspartate transaminase, and alkaline phosphatase), markedly attenuated CCl4-induced oxidative stress. The antifibrotic activity of SVE was evidenced by inhibiting the rise in hepatic hydroxyproline content and accumulation of collagen. This was confirmed by the ability of SVE to inhibit alterations in expression of the fibrosis-related genes Collagen Iα, matrixmetalloproteinase-2 (MMP-2), tissue inhibitor matrix metalloproteinase-2, and transforming growth factor beta 1. HPLC-qTOF-ESIMS analysis of SVE revealed the presence of 47 metabolites, among which 33 were tentatively identified. Conclusion: SVE exhibited hepatoprotective and antifibrotic activities in rats by enhancing the antioxidant capacity and regulating expression of fibrogenic mediators. Keywords:Antifibrotic activity, ESIMS, high performance liquid chromatography, liver, metabolite profiling, Solanum villosumView:PDF (3.39 MB)