gebhart/patrepeval huggingface dataset:
VRC01 provides sterilizing protection to non human primates from mucosal SHIV challenges (155.11) (2011)

Abstract VRC01 is an exceptionally broad neutralizing monoclonal antibody targeting the CD4 binding site of HIV gp120. It was isolated from an HIV-1 infected donor and is able neutralize over 90% of circulating HIV-1 isolates at high potency in vitro. Using a nonhuman primate lentiviral immunodeficiency virus infection model, we assessed the ability of VRC01 to protect animals from mucosal challenge with a pathogenic chimeric simian-human immunodeficiency virus (SHIV). The animals were first administered either VRC01 or a control IgG i.v. at a dose of 20 mg/kg and 2 days later were mucosally challenged with SHIV. We used two different mucosal routes - intrarectal (i.r.) and intravaginal (ivag) for mucosal challenge to mimic natural HIV exposures in humans. The plasma viral loads and sera VRC01 levels in animals were measured weekly by real time RT-PCR and ELISA, respectively. We observed that 3 out of the 4 animals in both the control groups were infected, with peak viremia occurring at week 2 for the i.r. challenge and at week 3 for the ivag challenge. In contrast, none of the 4 animals that received VRC01 got infected in both mucosal challenge groups (p=0.04). These results demonstrate that a broadly neutralizing CD4 binding site mAb, VRC01, protects against mucosal exposure to pathogenic viruses in vivo. This finding provides support for the CD4 binding site as a target of AIDS vaccine development and the possible use of VRC01 to prevent mucosal transmission of HIV-1 in humans.
Characterization of middle ear mucus glycoprotein in chronic secretory otitis media (1986)

Conference Article| August 01 1986 Characterization of middle ear mucus glycoprotein in chronic secretory otitis media JOHN E. FITZGERALD; JOHN E. FITZGERALD 1Department of Physiological Sciences, The Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, U.K. Search for other works by this author on: This Site PubMed Google Scholar GARY G. R. GREEN; GARY G. R. GREEN 1Department of Physiological Sciences, The Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, U.K. Search for other works by this author on: This Site PubMed Google Scholar JEFFREY P. PEARSON JEFFREY P. PEARSON 1Department of Physiological Sciences, The Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, U.K. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1986) 14 (4): 737–738. https://doi.org/10.1042/bst0140737a Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Cite Icon Cite Get Permissions Citation JOHN E. FITZGERALD, GARY G. R. GREEN, JEFFREY P. PEARSON; Characterization of middle ear mucus glycoprotein in chronic secretory otitis media. Biochem Soc Trans 1 August 1986; 14 (4): 737–738. doi: https://doi.org/10.1042/bst0140737a Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: CSOM, chronic secretory otitis media This content is only available as a PDF. © 1986 Biochemical Society1986 Article PDF first page preview Close Modal You do not currently have access to this content.
Analysis of Go-Back-N ARQ in Block Fading Channels (2007)

This work analyzes the throughput of Go-Back-N (GBN) in block fading, a model frequently used for slow fading wireless channels. We devise hidden Markov models and block transition probabilities for the block fading channel, allowing us to calculate the throughput of GBN with reliable feedback, as well as unreliable feedback. The advantages of this approach include generality: it applies to two-state as well as multi-state models for forward and reverse channels. Also, the results for both reliable and unreliable feedback are expressed in terms of probability matrices, which can be used as convenient building blocks in the analysis and simulation of larger systems. Simulations verify our analysis.
Trend-Aware Proactive Caching via Tensor Train Decomposition: A Bayesian Viewpoint (2021)

Content caching at base stations is an effective solution to cope with the unprecedented data traffic growth by prefetching contents near to end-users. To proactively servicing users, it is of high importance to extract predictive information from data requests. In this paper, we propose an accurate content request prediction algorithm for improving the performance of edge caching systems. In particular, we develop a Bayesian dynamical model through which a complex nonlinear latent temporal trend structure in the content requests can be accurately tracked and predicted. The dynamical model also leverages tensor train decomposition to capture content-location interactions to further enhance the accuracy of predictions. To infer the model’s parameters, we derive an approximation of the posterior distribution based on variational Bayes (VB) method with an embedded Kalman smoother algorithm. Based on the predictions of the proposed model, we design a cost-efficient proactive cooperative caching policy which adaptively utilizes network resources and optimizes the content delivery. The advantage of the proposed caching scheme is demonstrated via numerical results using two real-world datasets, which show that the developed Bayesian dynamical model substantially outperforms reference methods that ignore the temporal trends and content-location interactions.
Biosynthesis of the Cyanobacterial Light-Harvesting Polypeptide Phycoerythrocyanin Holo-α Subunit in a Heterologous Host (2002)

The entire pathway for the biosynthesis of the phycobiliviolin-bearing His-tagged holo-alpha subunit of the cyanobacterial photosynthetic accessory protein phycoerythrocyanin was reconstituted in Escherichia coli. Cyanobacterial genes encoding enzymes required for the conversion of heme to 3Z-phycocyanobilin, a precursor of phycobiliviolin (namely, heme oxygenase 1 and 3Z-phycocyanobilin:ferredoxin oxidoreductase), were expressed from a plasmid under the control of the hybrid trp-lac (trc) promoter. Genes for the apo-phycoerythrocyanin alpha subunit (pecA) and the heterodimeric lyase/isomerase (pecE and pecF), which catalyzes both the covalent attachment of phycocyanobilin and its concurrent isomerization to phycobiliviolin, were expressed from the trc promoter on a second plasmid. Upon induction, recombinant E. coli used endogenous heme to produce holo-PecA with absorbance and fluorescence properties similar to those of the same protein produced in cyanobacteria. About two-thirds of the apo-PecA was converted to holo-PecA. No significant bilin addition took place in a similarly engineered E. coli strain that lacks pecE and pecF. By using immobilized metal affinity chromatography, both apo-PecA and holo-PecA were isolated as ternary complexes with PecE and PecF. The identities of all three components in the ternary complexes were established unambiguously by protein and tryptic peptide analyses performed by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
Ortsaufgelöste Beobachtung von Schwingungsenergietransfer durch ein genetisch codiertes ultraschnelles Heizelement (2018)

Abstract Eine Reihe theoretischer Studien setzt allosterischen Informationstransfer in Proteinen in Beziehung zu definierten Schwingungsenergietransfer(VET)‐Pfaden. Experimentelle Evidenz für diese Pfade ist rar, da für ihre Erforschung eine punktuelle Injektion von Schwingungsenergie in Proteine, d. h. eine lokale Erhitzung, benötigt wird. Um diese Experimente zu ermöglichen, haben wir den ortsspezifischen Einbau der nichtkanonischen Aminosäure β‐(1‐Azulenyl)‐ l ‐alanin (AzAla) mithilfe des erweiterten genetischen Codes etabliert. AzAla stellt eine Ausnahme der Kasha‐Regel dar: während die Aminosäure bei S 1 ‐Anregung interne Konversion durchläuft und dadurch erhitzt, kann sie bei S 2 ‐Anregung als Fluoreszenzmarker dienen. Wir haben PDZ3, eine Interaktionsdomäne des postsynaptischen Proteins PSD‐95, an verschiedenen Positionen mit diesem ultraschnellen Heizelement ausgestattet. Unter Verwendung von ultraschneller IR‐Spektroskopie konnten wir VET, ausgehend von AzAla innerhalb des Proteins, zu einem gebundenen Liganden auf einer Pikosekunden‐Zeitskala beobachten. Dieser Ansatz basierend auf dem genetisch codierten Einbau von AzAla ebnet den Weg zu detaillierten Studien von VET und seiner Funktion in einer Vielzahl von Proteinen.
ESCA studies of the coordination state of aluminium in oxide environments (1997)

X-Ray photoelectron spectroscopy (known as XPS or ESCA) can differentiate between tetrahedral and octahedral Al atoms in inorganic solids. Although primarily used for the study of aluminosilicates, this semi-quantitative technique is applicable to other materials, irrespective of whether they contain aluminium in one or more coordinations with respect to oxygen. If less sensitive to the coordination of Al, the range of ESCA, a surface-oriented technique, is thus not very different from that of the bulk-oriented solid-state NMR. The procedures described here are successful with any high-resolution ESCA system, but are greatly facilitated by flood gun technology which removes insulator-induced charging shifts. Areas of potential difficulty are also discussed.
Production, Chemistry and Properties of Proteins (2011)

This chapter contains sections titled: Introduction Plant-Based Proteins Animal-Based Proteins Solution Casting of Proteins – an Overview Dry Forming of Protein Films Concluding Remarks References
A novel PV-modular multilevel converter for building integrated photovoltaics (2013)

The paper suggests a novel architecture of PV-powered Modular Multilevel Converter for building integrated photovoltaics. A grid-tied single-star configuration has been proposed. In particular, three different cell topologies have been analyzed and compared in terms of number of PV modules and rated voltages of semiconductor devices. Finally, a control strategy has been suggested in order to deliver to the grid the maximum power available from the PV modules at unity power factor.
ChemInform Abstract: Three‐Dimensional Ordered Arrays of 58x58x58 Å3 Hollow Frameworks in Ionic Crystals of M2Zn2‐Substituted Polyoxometalates. (2012)

Abstract M 2 Zn 2 ‐substituted polyoxometalates with M: Co, Ni, and Zn are characterized by single crystal XRD, UV/VIS spectroscopy, and magnetic measurements.
Proceedings of the 24th ACM SIGSPATIAL International Conference on Advances in Geographic Information Systems (2016)

These proceedings contain the papers from the 24th ACM SIGSPATIAL International Conference on Advances in Geographic Information Systems (ACM SIGSPATIAL 2016), held in the San Francisco Bay Area, California, USA, October 31 through November 3, 2016. The conference started as a series of symposia and workshops back in 1993 with the aim of promoting interdisciplinary discussions among researchers, developers, users, and practitioners and fostering research in all aspects of geographic information systems, especially in relation to novel systems based on geospatial data and knowledge. It provides a forum for original research contributions covering all conceptual, design, and implementation aspects of geospatial data ranging from applications, user interfaces and visualization, to data storage, query processing, indexing and data mining. The conference is the premier annual event of the ACM Special Interest Group on Spatial Information (ACM SIGSPATIAL).
Effect of intraarterial verapamil on the diameter of vasospastic intracranial arteries in patients with cerebral vasospasm (2006)

Administration of intraarterial verapamil does not cause a significant increase in the diameter of vasospastic vessels at the administered doses.
Coherent coupling of radiation modes in Mach-Zehnder electrooptic modulators (1992)

The effects of the radiation modes in Mach-Zehnder GaAs electrooptic modulators are studied using the standard beam propagation method (BPM). The radiation mode contribution to the energy transfer in the passive structure is studied as well as the performances of the active device under modulation. For compact weakly guiding structures, a good geometrical design that takes into account the propagation of the radiation modes is not only effective in loss reduction but also important for improving the design of the active structure. A 20% change of the modulator bias voltage is predicted with a shortening of the interaction length from 5 to 4.6 mm. This result is better understood when one considers the coherent coupling of the radiation modes in the structure.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
A Correlation Between Severity of Hail Damage in Potato Cultivars and the Nature of the Calcium in the Leaf and Stem Tissues1 (1971)

Abstract On May 17, 1969 a severe hail storm hit the Agricultural Research and Educational Center (A.R.E.C.) of the American University of Beirut located in the Beqa'a plain 80 km east of Beirut. Remarkable differences were observed in the rate of damage to plants in an evaluation trial of 22 potato cultivars planted in randomized complete block design.
Roles of LcrG and LcrV during Type III Targeting of Effector Yops by Yersinia enterocolitica (2001)

Yersinia enterocolitica target effector Yop proteins into the cytosol of eukaryotic cells by a mechanism requiring the type III machinery. LcrG and LcrV have been suggested to fulfill essential functions during the type III targeting of effector Yops. It is reported here that knockout mutations of lcrG caused mutant yersiniae to prematurely secrete Yops into the extracellular medium without abolishing the type III targeting mechanism (Los phenotype [loss of type III targeting specificity]). Knockout mutations in lcrV reduced type III targeting of mutant yersiniae but did not promote secretion into the extracellular medium (Not [no type III targeting]). However, knockout mutations in both genes caused DeltalcrGV yersiniae to display a Los phenotype similar to that of strains carrying knockout mutations in lcrG alone. LcrG binding to LcrV resulted in the formation of soluble LcrGV complexes in the bacterial cytoplasm. Membrane-associated, bacterial-surface-displayed or -secreted LcrG could not be detected. Most of LcrV was located in the bacterial cytoplasm; however, small amounts were secreted into the extracellular medium. These data support a model whereby LcrG may act as a negative regulator of type III targeting in the bacterial cytoplasm, an activity that is modulated by LcrG binding to LcrV. No support could be gathered for the hypothesis whereby LcrG and LcrV may act as a bacterial surface receptor for host cells, allowing effector Yop translocation across the eukaryotic plasma membrane.
Production of medically useful bromine isotopes via alpha-particle induced nuclear reactions (2017)

The cross sections of α-particle induced reactions on arsenic leading to the formation of 76,77,78Br were measured from their respective thresholds up to 37 MeV. Thin sediments of elemental arsenic powder were irradiated together with Al degrader and Cu monitor foils using the established stacked-foil technique. For determination of the effective α-particle energies and of the effective beam current through the stacks the cross-section ratios of the monitor nuclides 67Ga/66Ga were used. This should help resolve discrepancies in existing literature data. Comparison of the data with the available excitation functions shows some slight energy shifts as well as some differences in curve shapes. The calculated thick target yields indicate, that 77Br can be produced in the energy range Eα = 25 → 17 MeV free of isotopic impurities in quantities sufficient for medical application.
High Sulfur Content Organic/Inorganic Hybrid Polymeric Materials (2017)

The development of chemistry and processing methods for the utilization of elemental sulfur into polymeric materials provides a unique opportunity for addressing the substantial waste surfeit of sulfur generated via petroleum refining. Perhaps the most well-known reaction accessible from elemental sulfur is the ring-opening homopolymerization (ROP) of S8 to form polymeric sulfur. This is also one of the most widely known reactions conducted in molten sulfur, which is essentially a bulk free-radical polymerization process. A major breakthrough in the modern use of elemental sulfur for polymeric materials was the concept of using liquid sulfur as the reaction medium for chemical reactions. The preparation of well-defined high sulfur-content polymers is complicated by several synthetic and solubility issues associated with S-S bonds. To overcome these challenges, we pursued a synthetic strategy employing ring-opening metathesis polymerization (ROMP) of a cyclic sulfide functional norbornene.
ChemInform Abstract: Two Highly Oxygenated Eudesmanes and 10 Lignans from Achillea holosericea (2002)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Detecting Usable Planar Regions for Legged Robot Locomotion (2020)

Awareness of the environment is essential for mobile robots. Perception for legged robots requires high levels of reliability and accuracy in order to walk stably in the types of complex, cluttered environments we are interested in. In this paper, we present a usable environmental perception algorithm designed to detect steppable areas and obstacles for the autonomous generation of desired footholds for legged robots. To produce an efficient representation of the environment, the proposed perception algorithm is desired to cluster point cloud data to planar regions composed of convex polygons. We describe in this paper the end-to-end pipeline from data collection to generation of the regions, where we first compose an octree in order to create a more efficient data representation. We then group the leaves in the tree using a nearest neighbor search into a planar region, which is composed of the concave hull of points that is decomposed into convex polygons. We present a variety of environments, and illustrate the usability of this approach by the Atlas humanoid robots walking over rough terrain. We also discuss various challenges we faced and insights we gained in the development of this approach.
Expression of the cholecystokinin gene in pediatric tumors. (1992)

We have examined a wide range of cultured human tumor cell lines and found that a specific subset of tumors expresses the cholecystokinin (CCK) gene. All neuroepitheliomas (eight) and Ewing sarcoma (eight) cell lines that were tested express CCK RNA. In addition, two of six rhabdomyosarcoma cell lines also express the CCK gene, suggesting that rhabdomyosarcomas are probably heterogenous and that a subset may be similar to Ewing sarcoma and neuroepithelioma. Very few of the positive tumors express completely processed immunoreactive CCK. However, we have used a radioimmunoassay that detects the CCK precursor to demonstrate synthesis of CCK precursor-like peptides by all of the Ewing sarcoma and neuroepithelioma lines that were tested and by the rhabdomyosarcoma cell line that expresses CCK mRNA. These data demonstrate a consistent association of CCK gene expression with a specific group of human neoplasms. The data also add credence to the theory that Ewing sarcoma and neuroepithelioma are derived from the same transformed cell type. Finally, our results suggest that CCK gene expression may serve as a marker to distinguish these tumors, which are considered to be small-round-cell tumors of childhood, from other pediatric tumors.
Effects of age and Pax6 deficiency on mouse limbal stem cell function (2010)

The conventional view for corneal epithelial maintenance suggests that a stem cell population found in the limbus (at the rim of the cornea) produces daughter cells, called transient amplifying cells, which migrate centripetally. This limbal stem cell (LSC) hypothesis was recently questioned and the alternative model suggests that stem cells are present throughout the corneal epithelium. The main aims of this thesis were to investigate whether age and Pax6 genotype affect LSC function. Previous work with X-inactivation mosaics revealed radial stripes of β-galactosidase-expressing cells in the corneal epithelium (from about 5 weeks of age), which decreased with age and were reduced in Pax6+/- mice (a model for aniridia, a human eye disease). The reduction in Pax6+/- mice could be due to either reduced LSCs function or a more coarse-grained mosaicism caused by reduced cell mixing during development. Comparison of patch sizes in Pax6+/- and wild-type X-inactivation mosaics showed that patches were smaller in Pax6+/- cornea epithelia before the initiation of stripes (3 weeks of age). This implies that stripe-number reduction is not caused by reduced cell mixing, so an effect on LSC function remained a possibility. Thus, the numbers of label-retaining cells (putative stem cells) in Pax6+/- were compared to controls at 15 and 30 weeks old but they were not reduced at 30 weeks or in Pax6+/- mice, as had been predicted. The failure to demonstrate the predicted result suggests either that the hypothesis was incorrect or the experimental approach was inappropriate. Furthermore, it was discovered that mice expressing β-galactosidase under the keratin 5 promoter produced rare stripes in the corneal epithelium, which are likely to represent clonal lineages derived from individual stem cells. Older mice demonstrated a significantly lower frequency of stripes, a result compatible with the predicted reduction of active LSC with age. Pax6+/- corneas were highly abnormal and stripes were not formed properly, so direct comparison was not possible. Finally, pilot experiments with conditional expression of a reporter gene revealed the successful formation of a stripe, and hence provide a plausible alternative approach to compare stripe numbers reflecting active LSCs but the method has yet to be optimised. Overall, the results suggest that LSCs are reduced with age and support the limbal location of stem cells maintaining the corneal epithelium.