gebhart/patrepeval huggingface dataset:
Observation of the Decay B[subscript s][superscript 0] → [¯ over D][superscript 0] K[superscript +] K[superscript −] (2018)

The first observation of the B[subscript s][superscript 0]→[¯ over D][superscript 0]K[superscript +]K[superscript -] decay is reported, together with the most precise branching fraction measurement of the mode B[superscript 0]→[¯ over D][superscript 0]K[superscript +]K[superscript -]. The results are obtained from an analysis of pp collision data corresponding to an integrated luminosity of 3.0 fb[superscript -1]. The data were collected with the LHCb detector at center-of-mass energies of 7 and 8 TeV. The branching fraction of the B[superscript 0]→[¯ over D][superscript 0]K[superscript +]K[superscript -] decay is measured relative to that of the decay B[superscript 0]→[¯ over D][superscript 0]π[superscript +]π[superscript -] to be B(B[superscript 0]→[¯ over D][superscript 0]K[superscript +]K[superscript -])/B(B[superscript 0]→[¯ over D][superscript 0]π[superscript +]π[superscript -])=(6.9 ± 0.4 ± 0.3)%, where the first uncertainty is statistical and the second is systematic. The measured branching fraction of the B[subscript s][superscript 0]→[¯ over D][superscript 0]K[superscript +]K[superscript -] decay mode relative to that of the corresponding B[superscript 0] decay is B(B[subscript s][superscript 0]→[ ¯ over D][superscript 0]K[superscript +]K[superscript -])/B(B[superscript 0]→[ ¯ over D][superscript 0]K[superscript +]K[superscript -])=(93.0 ± 8.9 ± 6.9)%. Using the known branching fraction of B[superscript 0]→[¯ over D][superscript 0]π[superscript +]π[superscript ], the values of B(B[superscript 0]→[¯ over D][superscript 0]K[superscript +]K[superscript -])=(6.1 ± 0.4 ± 0.3 ± 0.3) × 10[superscript -5] and B(B[subscript s][superscript 0]→[¯ over D][superscript 0]K[superscript +]K[superscript -]) = (5.7 ± 0.5 ± 0.4 ± 0.5) × 10[superscript -5] are obtained, where the third uncertainties arise from the branching fraction of the decay modes B[superscript 0] →[¯ over D][superscript 0]π[superscript +]π[superscript -] and B[superscript 0]→[¯ over D][superscript 0]K[superscript +]K[superscript -], respectively.
The complexity of stoquastic local Hamiltonian problems (2008)

We study the complexity of the Local Hamiltonian Problem (denoted as LH-MIN) in the special case when a Hamiltonian obeys the condition that all off-diagonal matrix elements in the standard basis are real and non-positive. We will call such Hamiltonians, which are common in the natural world, stoquastic. An equivalent characterization of stoquastic Hamiltonians is that they have an entry-wise non-negative Gibbs density matrix for any temperature. We prove that LH-MIN for stoquastic Hamiltonians belongs to the complexity class \AM{}--- a probabilistic version of \NP{} with two rounds of communication between the prover and the verifier. We also show that $2$-local stoquastic LH-MIN is hard for the class \MA. With the additional promise of having a polynomial spectral gap, we show that stoquastic LH-MIN belongs to the class \POSTBPP=\BPPpath --- a generalization of \BPP{} in which a post-selective readout is allowed. This last result also shows that any problem solved by adiabatic quantum computation using stoquastic Hamiltonians is in PostBPP.
Simultaneous trichromatic fluorescence detection of proteins on Western blots using an amine‐reactive dye in combination with alkaline phosphatase‐ and horseradish peroxidase‐antibody conjugates (2003)

Three-color fluorescence detection methods are described based upon covalently coupling the dye 2-methoxy-2,4-diphenyl-2(2H)-furanone (MDPF) to proteins immobilized on poly(vinylidene difluoride) (PVDF) membranes, followed by detection of target proteins using alkaline-phosphatase-conjugated reporter molecules in combination with the fluorogenic substrate 9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) phosphate (DDAO-phosphate) as well as horseradish peroxidase-conjugated reporter molecules in combination with the new fluorogenic substrate Amplex Gold reagent. This results in all proteins in the profile being visualized as fluorescent blue signal, those detected specifically with the alkaline phosphatase conjugate appearing as fluorescent red signal and those detected specifically with the horseradish peroxidase conjugate appearing as fluorescent yellow signal. Using conventional secondary antibodies, two different targets may be identified as long as primary antibodies generated from two different species are used in the analysis. However, Zenon antibody labeling technology eliminates this restriction, permitting the simultaneous use of two different mouse monoclonal antibodies or two different rabbit polyclonal antibodies in the same electroblotting experiment. The trichromatic detection system is broadly compatible with UV epi-illuminators combined with photographic or charge-coupled device (CCD) cameras, and xenon-arc sources equipped with appropriate excitation/emission filters. Alternatively, the enzyme conjugates may be detected using a laser-based gel scanner. The trichromatic method permits detection of low nanogram amounts of protein and allows for unambiguous identification of two different target proteins relative to the entire protein profile on a single electroblot, precluding any requirement for running replicate gels that would otherwise require separate visualization of total proteins and subsequent alignment with multiple chemiluminescent or colorimetric signals generated on different electroblots.
Human optimization with moving optima (1989)

Abstract Earlier work had examined how humans optimize a two-variable function. This paper extends the study to optimization when the position of the optimum can change with time. Thirty student subjects performed a blind hill climbing task under two levels of display noise and three levels of movement of the optimum. Performance was studied using 13 measures, which were found to group into three factors, ‘deviation size’, ‘hits on target’ and ‘speed’. It was found that the movement of the optimum degraded performance once the general area of the optimum was reached. Display noise also degraded performance. Subjects with formal optimization training performed better on the ‘deviation size’ factor but had no advantage on ‘hits on target’ and ‘speed’ factors.
Computer-assisted mechanistic evaluation of organic reactions. 15. Heterocycle synthesis (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTComputer-assisted mechanistic evaluation of organic reactions. 15. Heterocycle synthesisMark G. Bures and William L. JorgensenCite this: J. Org. Chem. 1988, 53, 11, 2504–2520Publication Date (Print):May 1, 1988Publication History Published online1 May 2002Published inissue 1 May 1988https://pubs.acs.org/doi/10.1021/jo00246a020https://doi.org/10.1021/jo00246a020research-articleACS PublicationsRequest reuse permissionsArticle Views174Altmetric-Citations13LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Kallistatin reduces vascular senescence and aging by regulating microRNA‐34a‐SIRT1 pathway (2017)

Kallistatin, an endogenous protein, protects against vascular injury by inhibiting oxidative stress and inflammation in hypertensive rats and enhancing the mobility and function of endothelial progenitor cells (EPCs). We aimed to determine the role and mechanism of kallistatin in vascular senescence and aging using cultured EPCs, streptozotocin (STZ)-induced diabetic mice, and Caenorhabditis elegans (C. elegans). Human kallistatin significantly decreased TNF-α-induced cellular senescence in EPCs, as indicated by reduced senescence-associated β-galactosidase activity and plasminogen activator inhibitor-1 expression, and elevated telomerase activity. Kallistatin blocked TNF-α-induced superoxide levels, NADPH oxidase activity, and microRNA-21 (miR-21) and p16<sup>INK</sup><sup>4a</sup> synthesis. Kallistatin prevented TNF-α-mediated inhibition of SIRT1, eNOS, and catalase, and directly stimulated the expression of these antioxidant enzymes. Moreover, kallistatin inhibited miR-34a synthesis, whereas miR-34a overexpression abolished kallistatin-induced antioxidant gene expression and antisenescence activity. Kallistatin via its active site inhibited miR-34a, and stimulated SIRT1 and eNOS synthesis in EPCs, which was abolished by genistein, indicating an event mediated by tyrosine kinase. Moreover, kallistatin administration attenuated STZ-induced aortic senescence, oxidative stress, and miR-34a and miR-21 synthesis, and increased SIRT1, eNOS, and catalase levels in diabetic mice. Furthermore, kallistatin treatment reduced superoxide formation and prolonged wild-type C. elegans lifespan under oxidative or heat stress, although kallistatin's protective effect was abolished in miR-34 or sir-2.1 (SIRT1 homolog) mutant C. elegans. Kallistatin inhibited miR-34, but stimulated sir-2.1 and sod-3 synthesis in C. elegans. These in vitro and in vivo studies provide significant insights into the role and mechanism of kallistatin in vascular senescence and aging by regulating miR-34a-SIRT1 pathway.
Surrogate Based Centralized SON: Application to Interference Mitigation in LTE-A HetNets (2013)

A major challenge to the successful operation and maintainability of LTE-A network is to address competing objectives such as improved capacity, reduced operational costs and complexity. In this paper we use a surrogate function to model the functional relationships between noisy Key Performance Indicators (KPIs) and Radio Resource Management (RRM) parameters and subsequently use a pattern search algorithm for optimization under constraints. The proposed methodology is applied to solve a self-optimization problem for eICIC in an LTE-A HetNet deployment. Results obtained using a system level simulator show promising results for faster convergence towards optimal network configuration.
Choline dendrimers as generic scaffolds for the non-covalent synthesis of multivalent protein assemblies (2011)

A modular self-assembly strategy is presented that allows the non-covalent synthesis of multivalent protein dendrimers using the strong interaction between choline-functionalized dendrimers and the choline binding protein C-LytA. Choline dendrimers displaying fusion proteins of C-LytA and the collagen binding protein CNA35 represent attractive multivalent targeting ligands for collagen imaging.
Assessment of antitumor function of NK cells expanded with exosomes from K562.mb21 cells. (2017)

132 Background: NK cells can kill malignant cells to provide innate immunity against tumors. Due to their low abundance in blood, a focus is to expand NK cells ex vivo having enhanced anti-tumor cytotoxicity to be used as a treatment. Our group has pioneered a cell-free method using plasma membrane (PM) particles derived from K562 cells expressing 41BBL and membrane-bound IL-21 (K562.mb21) which were developed for NK cell expansion. Compared to feeder cell based methods for NK cell expansion, PM21-particles improve safety and allow for potential wide-spread dissemination, and also allows direct in vivo use. Exosomes, vesicles naturally secreted by cells, may yet be another novel feeder cell free way for NK cell expansion and may have further advantageous therapeutic dimensions. Methods: EX21-exosomes and PM21-particles were prepared from K562.mb21 cells and characterized by Nanosight and Western blot analysis. CD3-depleted PBMCs were cultured with EX21 for 14 days, NK cell amounts were monitored and media changed every 2-3 days. In vitro cytotoxicity against K562 cells were comparatively assessed for EX21-NK cells and PM21-NK cells. In vivo anti-tumor efficacy of EX21- and PM21-NK cells was assessed in NSG mice implanted ip with SKOV3_luc ovarian tumor cells (1 x 10 6 cells seeded for 4 days). SKOV3-bearing mice were treated with vehicle, or two doses of EX21-NK cells or PM21-NK cells (1 x 10 7 , in 5 day intervals), and with or without in vivo administration of EX21 (10 µg, 3x/week) or PM21-particles (600 µg, 3x/week). All groups were injected ip with IL-2 (10 KU, 3x/week). Survival analysis was performed with a Log-rank (Mantel-Cox) test. Results: NK cells cultured with EX21 expanded 530 fold (344-710) over 14 days compared to 735 fold (667-802) in presence of PM21-particles. Treatment of SKOV3 engrafted NSG mice with NK cells, expanded with either EX21 or with PM21, allowed significant ( &lt; 0.0001) increase in survival compared to untreated animals (41-44 vs 29 days post treatment). Ip delivery of EX21 to SKOV3 bearing mice had no effect on survival in either untreated control or EX21-NK cell treated groups. Conclusions: EX21 efficiently expands NK cells and EX21-NK cells have equal anti-tumor effect as PM21-NK cells, both in vitro and in vivo.
Practical Frequency Synchronization Scheme in an Open Software Radio TD-SCDMA System (2007)

This paper concerns how to achieve the initial frequency acquisition and frequency tracking in an open software radio TD-SCDMA platform, which consists of several common PCs and universal software radio peripheral. In this proposal, at the stage of frequency acquisition, the frequency difference between the base station and the terminal can be reduced step by step, by adjusting the size of segmental correlation window. Then at the stage of frequency tracking, the size of window is fixed as 32 chips to detect the residual frequency offset The simulation results and the software radio implementation prove that, the advantages of this invention are its wide frequency acquisition range, good performance and the robustness to multi-path fading.
The Role of Secretory Immunoglobulin A in the Natural Sensing of Commensal Bacteria by Mouse Peyer's Patch Dendritic Cells (2012)

The mammalian gastrointestinal (GI) tract harbors a diverse population of commensal species collectively known as the microbiota, which interact continuously with the host. From very early in life, secretory IgA (SIgA) is found in association with intestinal bacteria. It is considered that this helps to ensure self-limiting growth of the microbiota and hence participates in symbiosis. However, the importance of this association in contributing to the mechanisms ensuring natural host-microorganism communication is in need of further investigation. In the present work, we examined the possible role of SIgA in the transport of commensal bacteria across the GI epithelium. Using an intestinal loop mouse model and fluorescently labeled bacteria, we found that entry of commensal bacteria in Peyer's patches (PP) via the M cell pathway was mediated by their association with SIgA. Preassociation of bacteria with nonspecific SIgA increased their dynamics of entry and restored the reduced transport observed in germ-free mice known to have a marked reduction in intestinal SIgA production. Selective SIgA-mediated targeting of bacteria is restricted to the tolerogenic CD11c(+)CD11b(+)CD8(-) dendritic cell subset located in the subepithelial dome region of PPs, confirming that the host is not ignorant of its resident commensals. In conclusion, our work supports the concept that SIgA-mediated monitoring of commensal bacteria targeting dendritic cells in the subepithelial dome region of PPs represents a mechanism whereby the host mucosal immune system controls the continuous dialogue between the host and commensal bacteria.
Allosteric Supramolecular Coordination Constructs (2015)

Coordination chemistry is regularly used to generate supramolecular constructs with unique environments around embedded components to affect their intrinsic properties. In certain cases, it can also be used to effect changes in supramolecular structure reminiscent of those that occur within stimuli-responsive biological structures, such as allosteric enzymes. Indeed, among a handful of general strategies for synthesizing such supramolecular systems, the weak-link approach (WLA) uniquely allows one to toggle the frameworks' structural state post-assembly via simple reactions involving hemilabile ligands and transition metal centers. This synthetic strategy, when combined with dynamic ligand sorting processes, represents one of the few sets of general reactions in inorganic chemistry that allow one to synthesize spatially defined, stimuli-responsive, and multi-component frameworks in high to quantitative yields and with remarkable functional group tolerance. The WLA has thus yielded a variety of functional systems that operate similarly to allosteric enzymes, toggling activity via changes in the frameworks' steric confinement or electronic state upon the recognition of small molecule inputs. In this Perspective we present the first full description of the fundamental inorganic reactions that provide the foundation for synthesizing WLA complexes. In addition, we discuss the application of regulatory strategies in biology to the design of allosteric supramolecular constructs for the regulation of various catalytic properties, electron-transfer processes, and molecular receptors, as well as for the development of sensing and signal amplification systems.
Metal-mesh broadband anti-reflection coatings in the THz frequencies (2010)

We have recently designed, built and measured an alternative solution to the use of classical dielectric material in anti-reflection coatings. A metal-mesh pattern has been designed to cover a frequency band greater than 90%. We discuss measurements and illustrate the extension for bi-refringent materials.
A Novel Architecture for Content and Delivery Validation for IPTV Systems (2010)

In this paper, we describe a novel architecture for content and delivery validation for IPTV systems. The system generates multimedia events derived from IPTV content analysis and its delivery. Events are aggregated, filtered and, through a rules-based system, processed and prioritized to provide monitoring and alerting capabilities. Offline event processing along with a video archiving capability allows for detailed forensic analysis. The novelty of the approach includes the correlation and the combination of content-specific and network-specific events in a normalized representation to allow for complex forensic monitoring and alerting. Applications include content security assurance and metadata verification.
Evidence that phospholipase C is involved in the antitumour action of NSC768313, a new thieno[2,3-b]pyridine derivative (2016)

The results are consistent with a mechanism in which thieno[2,3-b]pyridine derivatives interact with PLC isoforms (possibly PLC-δ), which in turn affect the cellular dynamics of tubulin-β, inducing cell cycle arrest in G2-phase. We conclude that these compounds have novelty because of their PLC target and may have utility in combination with mitotic poisons for cancer treatment.
Use of a Cloned Multidrug Resistance Gene for Coamplification and Overproduction of Major Excreted Protein, a Transformation-Regulated Secreted Acid Protease (1988)

Malignantly transformed mouse fibroblasts synthesize and secrete large amounts of major excreted protein (MEP), a 39,000-dalton precursor to an acid protease (cathepsin L). To evaluate the possible role of this protease in the transformed phenotype, we transfected cloned genes for mouse or human MEP into mouse NIH 3T3 cells with an expression vector for the dominant, selectable human multidrug resistance (MDR1) gene. The cotransfected MEP sequences were efficiently coamplified and transcribed during stepwise selection for multidrug resistance in colchicine. The transfected NIH 3T3 cell lines containing amplified MEP sequences synthesized as much MEP as did Kirsten sarcoma virus-transformed NIH 3T3 cells. The MEP synthesized by cells transfected with the cloned mouse and human MEP genes was also secreted. Elevated synthesis and secretion of MEP by NIH 3T3 cells did not change the nontransformed phenotype of these cells.
Cholecystokinin (CCK)‐33 Stimulates Insulin Secretion from the Perfused Rat Pancreas: Studies on the Structure‐Activity Relationship (1988)

Cholecystokinin (CCK)-33 is known to stimulate insulin secretion. Presently, using the perfused rat pancreas, we have characterized the active site in the CCK-33 molecule that is responsible for this effect by the use of different CCK fragments. We found that CCK-33, CCK-8 and CCK-7 (1 nM) all significantly stimulated insulin secretion in the presence of 4.4 mM or 6.7 mM glucose. However, CCK-7 was much less potent than the longer forms. In contrast, CCK-4, CCK-6 and CCK-33 (1-21) had no effect on insulin secretion. We conclude that the shortest CCK-form that stimulates insulin secretion at 1 nM is the C-terminal heptapeptide CCK-7. However, CCK-8 is much more potent than CCK-7 in this respect.
Integrated Carbonate Matrix Acidizing Model for Optimal Treatment Design and Distribution in Long Horizontal Wells (2015)

Abstract Optimal design of matrix acidizing treatments in long horizontal wells represents a technical challenge for stimulation engineers. Acid coverage and distribution are particularly challenging because differences in formation permeability can be a predominant feature in horizontal wells. If acid is not effectively diverted in formations with high permeability anisotropy, some treated zones may become acid sinks while other zones are left with inadequate acid stimulation. Acid coverage and distribution depend on chemical and physical processes at different length scales. In the microscopic or pore scale in carbonate formations, acid creates flow paths of high permeability or wormholes which can affect the further penetration of the acid treatment downhole. In the macroscopic scale, fluid hydraulics in both wellbore and reservoir affect acid distribution as well. In this paper, we present an integrated model in both the microscopic and macroscopic scale to simulate multistage acidizing treatments in limited-entry and open-hole long horizontal wells. We propose a modified semi-analytical model to describe the wormholing phenomena and chemical diversion by using core flow test data. The reservoir heterogeneity in both vertical and horizontal directions is taken into consideration in this model. In addition, we establish a dynamic wellbore hydraulics model to characterize the fluid mechanics inside the wellbore with considerations for acid distribution, pressure drop due to friction loss, fluid rheology and fluid flow behavior. The integration of the elements mentioned above makes of this model a powerful tool to optimize critical parameters for matrix treatment design and distribution such as treatment interval length, diverter and acid volume, number of stages, and optimal rates. We present a case study to investigate the effects of chemical diversion, acid injection rate and volume on the performance of the treatment. The results of acid distribution, wormhole length and skin factor for each treatment interval as functions of time are analyzed. The evolution profiles of wellbore hydraulics during the acidizing treatment are also discussed.