gebhart/patrepeval huggingface dataset:
Supplementary Figure 8 from Reversal of NK-Cell Exhaustion in Advanced Melanoma by Tim-3 Blockade (2023)

&lt;p&gt;PDF file - 59K, Fig. S8. Tim-3 blockade has no effect in NK cell viability but increases the expression of CD16 in these cells.&lt;/p&gt;
Nonlinear Deformation of Multilayer MEMS Structures (1999)

Abstract We studied the deformation behavior of polysilicon/gold plate structures used for microelectromechanical systems (MEMS). We designed and had fabricated (using the MUMPS foundry process at Cronos Integrated Microsystems, Inc.) a series of test structures each consisting of a square polysilicon plate partially covered with gold films of different sizes and shapes. Deformed shapes of the polysilicon/gold plates were measured at room temperature using an interferometric microscope. For relatively small gold pads, the deformations were consistent with intuition and with predictions from a linear analysis; they deformed symmetrically, with roughly spherical curvature. With increasing gold pad size, though, the plates displayed nonsymmetrical deformations with roughly cylindrical curvature. A linear analysis was insufficient to explain this result, but a nonlinear one did, indicating a bifurcation due to the development of large deformations. We discuss this interesting, and potentially useful, physical phenomena and compare measured and predicted results.
Action recognition using invariant features under unexampled viewing conditions (2013)

A great challenge in real-world applications of action recognition is the lack of sufficient label information because of variance in the recording viewpoint and differences between individuals. A system that can adapt itself according to these variances is required for practical use. We present a generic method for extracting view-invariant features from skeleton joints. These view-invariant features are further refined using a stacked, compact autoencoder. To model the challenge of real-world applications, two unexampled test settings (NewView and NewPerson) are used to evaluate the proposed method. Experimental results with these test settings demonstrate the effectiveness of our method.
From Starch to Starch Microspheres: Factors Controlling the Microspheres Quality (2006)

Abstract Starch microparticle is a dosage form suitable for encapsulation of protein drugs. The microparticle characteristics can be influential factors for consecutive sustained drug release from the starch matrix and impact its ability to be coated by poly( D , L ‐lactide‐co‐glycolide). In this study, six types of starch microparticles were investigated and characterized. Desired qualities of the microspheres such as spherical form of the particles, well‐defined and sharp particle contours were coincident with coarse surface morphology, sharp and distinct peaks in DSC and X‐ray graphs. It was concluded that the quality of the starch microparticles was influenced by molecular properties of the starch material as well as type of buffer, and these factors were related to recrystallization kinetics, amount and quality of ordered/crystalline structure of final starch microspheres and their microscopic appearance.
Reconstitution of LDL with Lipophilic Fluorescein Derivatives: Quantitative Analysis of the Receptor Activity of Human Lymphocytes (1983)

Low density lipoprotein (LDL), the major cholesterol transport protein in human plasma, consists of an apolar core of cholesteryl esters surrounded by a polar shell containing phospholipids, unesterified cholesterol and protein. In the current paper we report the absorption and fluorescence spectra of members of a new class of lipophilic fluorescein derivatives which were designed to be reconstituted into the core of LDL in place of the native cholesteryl esters. One of these derivates, cholesteryl 12-O-[methyl 3-O-methyl-5' (6')-carboxyfluorescein]ricinoleyl carbonate (MMC) was reconstituted into the core of LDL. The resultant fluorescent reconstituted LDL was used in conjunction with flow cytometry to quantify the LDL receptor activity of fresh blood lymphocytes derived from normal individuals and from patients with the heterozygous and homozygous forms of familial hypercholesterolemia (FH). The LDL receptor activities of the heterozygous and homozygous FH lymphocytes were approximately 37% and 1% of normal, respectively. LDL reconstituted with these lipophilic fluorescein derivatives will be valuable in studying LDL metabolism and may be useful for the diagnosis of FH.
Microstructures and Electrical Properties of Random-Oriented (Bi,La)<sub>4</sub>Ti<sub>3</sub>O<sub>12</sub> Thin Film Deposited by Pulsed-DC Sputtering Method (2009)

Ferroelectric properties of Lead-free (Bi,La)4Ti3O12 (BLT) films were evaluated on the newly developed MTP (Merged Top-electrode and Plate-line) cell structure. The BLT film was deposited by pulsed-DC sputtering method on a buried Pt/IrOx/Ir bottom electrode stack with W-plug. The BLT composition in the sintered sputtering target was Bi4.8La1.0Ti3.0O12. However, the deposited film composition was about Bi4.0La1.0Ti3.0O12 after the heat treatment of crystallization at 700°C/O2/30sec. And grains of the BLT film were randomly oriented and uniformly small ellipsoidal shape (long direction: ~100nm, short direction: ~20 nm). The remnant polarization (2Pr) and the leakage current density measured in the 100nm-thick BLT film were about 21 C/cm2 and 3 ×10-5 A/cm2 at 3 V, respectively. The fatigue loss was about 10% of the initial polarization value after 1×1011 fatigue cycles.
Capillary electrophoresis/mass spectrometry (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCapillary Electrophoresis/Mass SpectrometryRichard D. Smith, Jon H. Wahl, David R. Goodlett, and Steven A. HofstadlerCite this: Anal. Chem. 1993, 65, 13, 574A–584APublication Date (Print):July 1, 1993Publication History Published online31 May 2012Published inissue 1 July 1993https://pubs.acs.org/doi/10.1021/ac00061a717https://doi.org/10.1021/ac00061a717research-articleACS PublicationsRequest reuse permissionsArticle Views312Altmetric-Citations131LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Evaluation of safety, tolerability and efficacy of temsirolimus in patients (pts) with relapsed or refractory mantle cell lymphoma (rel/refr MCL) in routine clinical practice (2016)

Background: Temsirolimus (TEM), an mTOR inhibitor, is approved in the EU for the treatment of pts with rel/refr MCL. A pivotal study demonstrated significantly longer progression free survival (PFS) with TEM (175 mg weekly for 3 weeks followed by 75 mg weekly) in rel/refr MCL pts compared to investigatoŕs choice therapy (4.8 vs 1.9 months (mo); P = .0009, HR 0.04). To evaluate safety and efficacy of TEM in this rare tumor entity, further collection of data in a post-approval prospective non-interventional trial is useful. Methods: A German multicenter registry for rel/refr MCL pts treated with TEM was started in Germany in Oct 2009 (NCT00700258). Objectives are the evaluation of safety, tolerability and anti-tumor activity of TEM as well as patient's profile including comorbidities, characteristics, and the sequence of systemic therapies. Results: From Oct 2009 to Feb 2018, 56 pts were recruited in 31 study sites. Baseline characteristics are available for 56 pts: 69.6% male; median age 74.4 years; ECOG PS 0 or 1 in 83.6%, PS 2 in 16.4%. According to MIPI score (n = 54), 20.4%, 33.3%, and 46.3% are classified as low, intermediate, and high risk at the time of enrollment. Median number of prior therapies is 2 with 44.7% treated in ≥ 4th line. Drug related adverse and serious adverse events are observed in 67.9% and 12.5% of pts, respectively. Most common drug-related toxicities of any grade (incidence ≥ 15%) in the following categories: blood/lymphatic system disorders (48.2%), gastrointestinal disorders (28.6%), general disorders (23.2%), and skin/subcutaneous tissue disorders (17.9%). Efficacy analyses are available for 40 assessable pts with an objective response rate (ORR) of 30%, a clinical benefit rate of 60% and PD in 40% of the pts. Median PFS for all pts is 3.7 mo and for pts who achieved an OR with TEM 6.3 mo. Median OS for all pts is 14.8 mo. Conclusions: The registry was started to evaluate the safety and efficacy of TEM in pts with rel/refr MCL in routine clinical practice. 46.3% high-risk pts were included in the analysis. In this poor-prognosis patient population, TEM showed a predictable, manageable tolerability profile. Efficacy parameters were consistent with published phase III data. Clinical trial identification: NCT00700258. Legal entity responsible for the study: Pfizer Pharma GmbH. Funding: Pfizer Pharma GmbH. Disclosure: M. Woike, G. Krekeler: Employee: Pfizer Pharma GmbH. All other authors have declared no conflicts of interest.
Adult Hypolactasia, Milk Consumption, and Age-specific Fertility (1994)

Beta-galactosidase (lactase) allows the digestion of lactose as its component sugars, galactose and glucose. Considerable variation exists worldwide in the prevalence of adults who lose the ability to digest lactose after infancy (hypolactasia) as well as in the amount of milk products they consume. Clearly, those populations in which hypolactasia is infrequent and milk consumption high will have greater dietary exposure to galactose. Because there is clinical and experimental evidence that galactose may be toxic to ovarian germ cells, the authors sought to determine whether age-specific fertility rates in various countries correlate with the prevalence of adult hypolactasia and per capita milk consumption by analysis of published data on these variables. The authors found significant correlations among these variables such that fertility at older ages is lower and the decline in fertility with aging is steeper in populations with high per capita consumption of milk and greater ability to digest its lactose component. These demographic data add to existing evidence that dietary galactose may deleteriously affect ovarian function.
Packaging and thermal management of high-power, slab-coupled optical waveguide laser arrays for beam combining (2008)

Linear arrays of slab coupled optical waveguide lasers (SCOWL) are nearly ideally suited for beam combining of array elements. SCOWL array elements have very high brightness, low divergence, nearly-diffraction-limited optical output beams with Watt-level performance. Both wavelength beam combining (WBC) and coherent beam combining (CBC) of SCOWL array elements have been successfully demonstrated. In this presentation, the packaging techniques developed to ensure proper performance of SCOWL arrays will be described, with particular emphasis on the application to beam combining. Arrays up to 1.2 cm wide, containing 120 elements on 100-mum element-to-element pitch have been demonstrated and used for WBC experiments. Aggressive thermal management was required in order to ensure optimum CW performance of these arrays and to minimize thermal cross talk between array elements. Commercial high performance micro impingement coolers (MIC) were used to accomplish this task. A numerical thermal model was constructed and used to investigate the thermal performance for several alternative packaging schemes using the MIC coolers. Assembly procedures were also developed in order to promote uniform optical performance of SCOWL array elements. These techniques resulted in minimal contraction (~2 mum) and smile (~1 mum) of the laser bar during the packaging process, which were sufficiently small to have minimal impact on the resultant WBC beam quality. CBC of SCOWL arrays required the ability to adjust the phase of the individual array elements. Packaging providing both individual electrical addressability of SCOWL array elements and good thermal performance has been developed and demonstrated, allowing for phase control by current adjustment. Individually addressable, 10-element SCOWL arrays have been demonstrated with total uncombined output power levels of >10 W CW. Coherent combining of ~7 W CW of optical power has been demonstrated from these 10-element SCOWL arrays.
Changes in protein kinase C isozymes in the rat hippocampal formation following hippocampal lesion (1993)

The cellular and regional distribution of the four protein kinase C (PKC) isoforms in the rat hippocampal formation and the response of PKC to lesions were determined by employing immunohistochemical and immunochemical techniques with antibodies specific to PKC(alpha), -(beta I), -(beta II), and -(gamma). PKC(alpha) intensely stained the periphery of the pyramidal cell in the stratum pyramidale. The granule cells, glial cells, and mossy fibers were anti-PKC(alpha) negative. The cytoplasm, axons, and dendrites of basket cells and interneurons in the hilus were labeled with anti-PKC(alpha). Anti-PKC(beta I) immunoreactivity was localized on the periphery of pyramidal cells and interneurons of the hilus, as well as the oriens, radiatum, and molecular layers of the CA regions. Anti-PKC(beta II) immunoreactivity was mainly cytoplasmic, extending into the dendrites in the hippocampal pyramidal cells and the dentate granule cells, and also in some glial cells. In the stratum radiatum of the CA1, anti-PKC(gamma) immunoreactivity localized to the pyramidal cell cytoplasm, extending into the dendrites. Following fimbria-fornix (FF) lesions, the anti-PKC(alpha) and -(beta I) staining of the pyramidal cell periphery was markedly reduced. The anti-PKC(gamma) staining of the pyramidal and granular cells of the dentate gyrus was reduced whereas the interneuron staining in the hilus was increased. In the FF-lesioned hippocampus, anti-PKC(alpha) and anti-PKC(beta II) labeled reactive glial cells, whereas anti-PKC(beta I) and -(gamma) did not. Quantitative Western blot analysis revealed a dramatic increase in the particulate/total PKC for all isozyme forms, although the total levels of PKC, except PKC(gamma), did not change following FF lesions. The PKC(gamma) concentration doubled after FF lesions. Perforant path lesions resulted in a marked alteration in the neuronal staining in dentate gyrus with anti-PKC(alpha) and -(beta I) and in increased numbers of anti-PKC(alpha)- and anti-PKC(beta II)-positive glial cells. Anti-PKC(gamma) staining did not change noticeably. The total PKC concentration did not change for isozymes alpha, beta I, and gamma, but PKC (beta II) concentration increased by 48% following perforant path lesions as detected by Western blot analysis. The particulate/total PKC decreased for all four isozymes although the reduction in PKC(beta I) concentration was not statistically significant. This change in PKC compartmentalization is in marked contrast to an increased level of particulate PKC following FF lesions. Thus, the effects of deafferentation and deafferentation for each PKC isoform were different.
Solid State Laser Driver for an ICF Reactor (1989)

A conceptual design is presented of the main power amplifier of a multi-beamline, multi-megawatt solid state ICF reactor driver. Simultaneous achievement of useful beam quality and high average power is achieved by a proper choice of amplifier geometry. An amplifier beamline consists of a sequence of facepumped rectangular slab gain elements, oriented at the Brewster angle relative to the beamline axis, and cooled on their large faces by helium gas that is flowing subsonically.1 The infrared amplifier output radiation is shifted to an appropriately short wavelength (<500 nm) using nonlinear crystals that are also gas cooled. We project an overall driver efficiency >10% (including all flow cooling input power) when the amplifiers are pumped by efficient high-power AlGaAs semiconductor laser diode arrays.
The impact of nonlinearity during femtosecond pulse compression in fibers on continuum coherence (2008)

Amplified erbium-fiber-laser pulses compressed in large-mode-area fiber show significantly reduced nonlinearity compared to standard-single-mode fiber. Consequently, supercontinuum generated with the pulses compressed in large-mode-area fiber show a 10 dB increase in cross-coherence fringe visibility.
Predicting the Force Needed to Create a Compression Seal in an Ultra-Thin Elastoviscoplastic Membrane (2016)

In this paper, a finite element model is developed, and experimentally validated, for predicting the force required to produce a compression seal between a polycarbonate sealing boss and a 25 μm thick elastoviscoplastic hemodialysis membrane. This work leverages previous efforts to determine the conditions for hermetic sealing in a microchannel hemodialyser fabricated using hot-embossed polycarbonate microchannel laminae containing sealing boss features. Methods are developed for mechanically characterizing the thin elastoviscoplastic hemodialysis membrane. Experimental data for assessing the depth of penetration into the membrane as a function of force show an R2 value of 0.85 showing good repeatability. The average percent error was found to be −8.0% with a range between −21.9% and 4.4% error in the strain region of interest.
Detection of chromosomal translocations in formalin‐fixed paraffin‐embedded (FFPE) leukemic specimens by digital expression profiling (2015)

Digital expression profiling can be used to measure translocation t(9;22), t(15;17), and t(8;21) in FFPE samples and is useful when a confirmatory test from a FFPE sample is necessary.
Investigating the tumor immune infiltrate for populations that predict immune-related adverse events (irAEs) in patients receiving PD-1 inhibitors. (2020)

3116 Background: The mechanistic relationship between clinical benefit and immune-related adverse events (irAEs) in response to immune checkpoint inhibitors (ICIs) remains unclear, with several clinical studies reporting that irAEs are biomarkers of responses. Single-cell RNA sequencing (scRNAseq) analysis of tumors from patients with advanced melanoma before and after treatment with ICIs have identified immune cells that correlate with response to ICIs. We sought to evaluate if these populations were also associated with irAEs. Methods: A published scRNAseq data set generated with the Smart-Seq2 protocol (Sade-Feldman M, et al. Cell 2018.) was re-analyzed, stratified by two definitions of irAEs: (1) toxicity requiring systemic immunosuppression (prednisone &gt; 10mg/day) or (2) systemic immunosuppression and/or endocrinopathy. Unbiased single-cell analysis was performed, followed by sub-clustering of T cell populations. The percentage of cells in each cluster was determined on a per sample basis. Results: 13,184 immune cells from 39 samples collected from 25 patients were re-analyzed. 27 samples were from patients who did not respond to ICIs, while 12 samples came from responding patients. 21 samples came from patients who required immunosuppression, 5 samples from patients with isolated thyroiditis, and 13 samples from patients who met neither irAE criteria. Unsupervised scRNAseq analyses focused on ICI efficacy re-capitulated published associations between response and populations that included B-cells (p &lt; 0.01) and TCF7 expressing T-cells (p &lt; 0.01). While these cell populations were not associated with either definition of toxicity, we observed a non-Treg CD4 expressing T cell population (0.8-10.5% cells/sample) that positively correlated with either definition of toxicity (p &lt; 0.05) but not efficacy. Conclusions: In a patient cohort with advanced melanoma, tumor-infiltrating immune cell populations associated with response to ICI therapy were not associated with irAEs. This suggests that biomarkers of ICI response may not function as biomarkers of irAEs, and ongoing analysis will seek to validate this result. Understanding the differences between ICI response and irAEs may identify new therapeutic targets for maximizing efficacy while mitigating toxicity.
Fabrication of Freestanding Pb(Zr,Ti)O Film Microstructures Using Ge Sacrificial Layer (2012)

A high quality freestanding Pb(Zr,Ti)O3 (PZT) film with various micropatterns was fabricated using a Ge sacrificial layer in order to meet the need of micro-electromechanical system (MEMS) applications. A flat Pt film was released from a Si substrate by etching the Ge layer (∼1 µm) between the Pt film and the Si substrate. After that, PZT was deposited on the freestanding Pt film by low temperature rf-magnetron sputtering and then annealed. The obtained freestanding PZT film showed strong (100) orientation and good ferroelectric property. This might be due to the characteristics of the developed process, for example, the lattice parameter change of the Pt film, a small heat capacity in the annealing process of PZT, and an absence of clamping effect from the substrate.
Input Files and Procedures for Analysis of SMA Hybrid Composite Beams in MSC, Nastran and ABAQUS (2013)

A thermoelastic constitutive model for shape memory alloys (SMAs) and SMA hybrid composites (SMAHCs) was recently implemented in the commercial codes MSC.Nastran and ABAQUS. The model is implemented and supported within the core of the commercial codes, so no user subroutines or external calculations are necessary. The model and resulting structural analysis has been previously demonstrated and experimentally verified for thermoelastic, vibration and acoustic, and structural shape control applications. The commercial implementations are described in related documents cited in the references, where various results are also shown that validate the commercial implementations relative to a research code. This paper is a companion to those documents in that it provides additional detail on the actual input files and solution procedures and serves as a repository for ASCII text versions of the input files necessary for duplication of the available results.