gebhart/patrepeval huggingface dataset:
In Vitro Growth in Acute Myeloblastic Leukemia: Clinico-Biological Correlations (1999)

In the present review we analyse the current knowledge about the growth properties of AML progenitor cells and their relationship with other clinico-biological characteristics of the disease. Leukaemic colony forming unit L-CFU is considered to be the clonogenic cell in AML and more immature than the blast cell population. Our studies have shown that in leukaemic hematopoiesis colony forming cells can exist among both cell fractions CD34+ and CD34-. Optimal "in vitro" proliferation of L-CFU is dependent upon the addition of exogenous growth factors. However, it has been observed that leukaemic progenitor cells frequently display a certain degree of autonomous proliferation. In order to quantify the "in vitro" behaviour of L-CFU, we have explored 3 parameters: 1) plating efficiency (PE); 2) autonomous growth (AG); and 3) autonomous proliferative index (API) which was calculated as AG divided by PE and we have correlated them with other clinico-biological data. According to the FAB classification we could observe that patients with M3 subtype showed an higher PE than other AML subgroups and a significantly lower API. Regarding CD34 expression we observed that AG was enhanced in CD34+ cases and also in those showing a higher rh123 elimination. In order to determine whether PE could condition clinical evolution, we analysed this parameter in a large series of patients but failed to demonstrate any relationship. By contrast, we observed that patients who displayed a higher API showed a shorter survival than patients with lower API (18% vs 48% surviving at 3 years). We have also shown that abnormalities in the CFU-GM growth pattern could be associated with risk the of relapse in AML patients; a switch from normal to abnormal "in vitro" growth should alert us. But for the assessment of the real value of these analyses sequential follow-up studies are mandatory. In summary, cell culture studies contribute not only to a better understanding of leukaemic hematopoiesis but may also contribute to better disease monitoring.
3D Fakes: Chemical Fingerprinting in Additive Manufacturing, from Pharmaceuticals to Engines (2017)

The rise of 3D printing brings with it manufacturing opportunity and new challenges in intellectual property protection. We address the use of chemical fingerprinting as a strategy to validate genuine product and protect against counterfeits.
Tympanoplastik – Neues und neu Beleuchtetes (2017)

Techniques and biomaterials for reconstructive middle ear surgery are under steady, ongoing development. At the same time, clinical post-surgery results are evaluated to determine success or failure of the therapy. Routine quality assessment and assurance is of growing importance in the medical field, and therefore also in middle ear surgery. The exact definition and acquisition of outcome parameters is essential for both a comprehensive and detailed quality assurance. These parameters are not the audiological results alone, but also additional individual parameters which influence the postoperative outcome after tympanoplasty. Selection of patients and the preoperative clinical situation, the extent of the ossicular chain destruction, the chosen reconstruction technique and material, the audiometric frequency selection and the observational interval are only some of them. If these parameters are not well documented comparative analyses between different studies are of limited value. The present overview aims to describe, compare and evaluate some of the existing assessment and scoring systems for middle ear surgery. Additionally, new methods for an intraoperative quality assessment in ossiculoplasty and the postoperative evaluation of suboptimal hearing results with imaging techniques are available. In the area of implant development functional elements were integrated in prostheses to enable not only good sound transmission but also compensation of occurring atmospheric pressure changes. In combination with other components for ossicular repair they can be used in a modular manner which, so far show experimentally and clinically promising results.
Algebraic Approach and Optimal Physical Clusterization in Interpolation Problems of Artificial Intelligence (2000)

Perceptron-type interpolation systems of artificial intelligence are considered. A concept of optimal physical clusterization allows us to divide a second layer of hidden units into the compact sets of units (clusters). Then, an algebraic approach developed for pattern recognition systems may be extended to other systems. To solve the problems of process forecasting, a data sample should be transformed into a single-moment sample.
An Innovative Anode Interface Combination for Perovskite Solar Cells with Improved Efficiency, Stability, and Reproducibility (2022)

Rational design and engineering of top interface layers with combined properties of effective passivation, high thermal‐ and photo‐stability are effective methods to advance the commercialization of perovskite photovoltaics. Here, an innovative anode interface combination is developed based on alcohol‐dispersed poly(3‐hexylthiophene‐2,5‐diyl) (P3HT) nanoparticles as the hole transport material and chlorobenzene‐dissolved trioctylphosphine oxide (TOPO) as the passivation agent. It is shown that instead of the commonly used 2D passivation ligands, TOPO‐passivated perovskite films exhibit greatly improved thermal stability. Furthermore, the passivation contributes to an enhanced carrier lifetime and reduced surface trap density, yielding an improvement in the quasi‐fermi‐level splitting of 57 meV. To maintain surface passivation during solution processing of further layers, it is necessary to develop a hole transport layer that can be processed from orthogonal solvents. P3HT nanoparticles formulated in alcoholic media fully meet this requirement, clearly benefitting from their high vertical conductivity and extremely low contact resistance with carbon electrodes. Based on this configuration, device efficiency of up to 18.4% is demonstrated for perovskite solar cells with fully solution‐processed carbon electrodes, along with significantly improved device stability and reproducibility.
Synthesis, Structure, and C-H Bond Activation Chemistry of (.eta.6-arene)Ru(H)2(SiMe3)2 Complexes (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis, Structure, and C-H Bond Activation Chemistry of (.eta.6-arene)Ru(H)2(SiMe3)2 ComplexesPeter I. Djurovich, Patrick J. Carroll, and Donald H. BerryCite this: Organometallics 1994, 13, 7, 2551–2553Publication Date (Print):July 1, 1994Publication History Published online1 May 2002Published inissue 1 July 1994https://pubs.acs.org/doi/10.1021/om00019a005https://doi.org/10.1021/om00019a005research-articleACS PublicationsRequest reuse permissionsArticle Views425Altmetric-Citations50LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Phase Diagram of Solutions of Associative Polymers (2004)

An analytical expression for the free energy of solutions of associative polymers is obtained in the entire polymer concentration−temperature range. This expression is derived under assumption that both local intrachain loops as well as interchain associations contribute to combinatorial part of the system free energy. In a good solvent regime, theory takes into account the swelling of polymer chains and suppression of pairwise associations due to strong monomer−monomer repulsive interactions. Analysis of the general expression of the system free energy shows that associations between functional groups can lead to a collapse of a chain and phase separation even under good solvent conditions for polymer backbone. In a good solvent, changing the fraction of associative groups per chain can trigger a phase separation.
Shear stress upregulates regeneration‐related immediate early genes in liver progenitors in 3D ECM‐like microenvironments (2017)

The role of fluid stresses in activating the hepatic stem/progenitor cell regenerative response is not well understood. This study hypothesized that immediate early genes (IEGs) with known links to liver regeneration will be upregulated in liver progenitor cells (LPCs) exposed to in vitro shear stresses on the order of those produced from elevated interstitial flow after partial hepatectomy. The objectives were: (1) to develop a shear flow chamber for application of fluid stress to LPCs in 3D culture; and (2) to determine the effects of fluid stress on IEG expression in LPCs. Two hours of shear stress exposure at ∼4 dyn/cm<sup>2</sup> was applied to LPCs embedded individually or as 3D spheroids within a hyaluronic acid/collagen I hydrogel. Results were compared against static controls. Quantitative reverse transcriptase polymerase chain reaction was used to evaluate the effect of experimental treatments on gene expression. Twenty-nine genes were analyzed, including IEGs and other genes linked to liver regeneration. Four IEGs (CFOS, IP10, MKP1, ALB) and three other regeneration-related genes (WNT, VEGF, EpCAM) were significantly upregulated in LPCs in response to fluid mechanical stress. LPCs maintained an early to intermediate stage of differentiation in spheroid culture in the absence of the hydrogel, and addition of the gel initiated cholangiocyte differentiation programs which were abrogated by the onset of flow. Collectively the flow-upregulated genes fit the pattern of an LPC-mediated proliferative/regenerative response. These results suggest that fluid stresses are potentially important regulators of the LPC-mediated regeneration response in liver.
CD4 cell count at initiation of ART, long-term likelihood of achieving CD4 >750 cells/mm3 and mortality risk (2016)

Abstract Objectives We sought to evaluate associations between CD4 at ART initiation (AI), achieving CD4 &gt;750 cells/mm3 (CD4 &gt;750), long-term immunological recovery and survival. Methods This was a prospective observational cohort study. We analysed data from ART-naive patients seen in 1996–2012 and followed ≥3 years after AI. We used Kaplan–Meier (KM) methods and log-rank tests to compare time to achieving CD4 &gt;750 by CD4 at AI (CD4-AI); and Cox regression models and generalized estimating equations to identify factors associated with achieving CD4 &gt;750 and mortality risk. Results Of 1327 patients, followed for a median of 7.9 years, &gt;85% received ART for ≥75% of follow-up time; 64 died. KM estimates evaluating likelihood of CD4 &gt;750 during 5 years of follow-up, stratified by CD4-AI &lt;50, 50–199, 200–349, 350–499 and 500–750, were 20%, 25%, 56%, 80% and 87%, respectively (log-rank P &lt; 0.001). In adjusted models, CD4-AI ≥200 (versus CD4-AI &lt;200) was associated with achievement of CD4 &gt;750 [adjusted HR (aHR) = 4.77]. Blacks were less likely than whites to achieve CD4 &gt;750 (33% versus 49%, aHR = 0.77). Mortality rates decreased with increasing CD4-AI (P = 0.004 across CD4 strata for AIDS causes and P = 0.009 for non-AIDS death causes). Among decedents with CD4-AI ≥50, 56% of deaths were due to non-AIDS causes. Conclusions Higher CD4-AI resulted in greater long-term CD4 gains, likelihood of achieving CD4 &gt;750, longer survival and decreased mortality regardless of cause. Over 80% of persons with CD4-AI ≥350 achieved CD4 &gt;750 by 4 years while 75% of persons with CD4-AI &lt;200 did not. These data confirm the hazards of delayed AI and support early AI.
Telomeres and telomerase in human leukemias. (1996)

There is increasing evidence supporting the hypothesis that telomere shortening both in vitro and in vivo, is the clock that counts cell divisions and determines the onset of cellular senescence. Cells that overcome the normal senescence mechanisms do so by stabilizing telomere length, probably due to the activity of telomerase, a ribonucleoprotein enzyme that synthesizes telomeric repeats. Most human primary tumors contain telomerase, while the cells of most normal tissues lack this activity. A hypothesis gaining prominence is that the activation of telomerase is necessary for the sustained growth of most solid tumors. Since normal hematopoietic stem cells and some of their progeny already express telomerase activity, it is important to consider whether or not telomere shortening and telomerase activity play any role in cancer progression in various forms of leukemia. This review includes a discussion of the utility of telomere length and/or telomerase activity measurements in the diagnosis and prognosis of leukemia as well as the potential value of antitelomerase therapy for the leukemias.
Poly(ether amide) segmented block copolymers with adipic acid based tetraamide segments (2007)

Abstract Poly(tetramethylene oxide)‐based poly(ether ester amide)s with monodisperse tetraamide segments were synthesized. The tetraamide segment was based on adipic acid, terephthalic acid, and hexamethylenediamine. The synthesis method of the copolymers and the influence of the tetraamide concentration, which was varied between 3 and 44 wt %, were studied. The copolymers were characterized by differential scanning calorimetry and temperature‐dependent Fourier transform infrared, small‐angle X‐ray scattering, atomic force microscopy, and dynamic mechanical thermal analysis. The monodisperse tetraamide segments crystallized fast, forming crystalline ribbons with high aspect ratios, and the crystallinity of the tetraamide segments in the copolymers was typically 90%. The glass‐transition temperature of the poly(tetramethylene oxide) phase was low (−65 to −70°C), and the modulus in the plateau region of the copolymers was virtually temperature‐independent. With increasing content of crystallizable amide segments in the copolymer, the storage modulus at room temperature increased from 1 to 102 MPa. This strong increase in the modulus with the tetraamide content could be approximated with a model for fiber‐reinforced polymers. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci 2007
Channel Measurements and Path loss Modeling for Indoor THz Communication (2020)

To explore the eventual deployment of communication systems in Terahertz (THz) band (0.1-10 THz) frequencies, extensive channel measurement campaigns are essential. In this regard, we conducted an indoor line-of-sight (LoS) measurement campaign up to 5.5 m on a THz channel sounding system that covers 140-220GHz. We use a frequency-domain channel sounder that is based on a vector network analyzer (VNA) and frequency extenders for these measurements. Using the log-distance path loss model, we estimate the values of path loss exponent and the fading distribution standard deviations. The power delay profile analysis of our measurements shows that there are negligible multipaths in the LoS channels for the current scenario. Our results provide a platform for future exploration of THz band communication in the 140-220 GHz frequency range.
Potentiation of diethylstilbestrol-induced alterations in the female mouse reproductive tract by transforming growth factor-α transgene expression (1996)

Neonatal estrogen exposure causes numerous abnormalities in the female reproductive tract, including carcinogenesis. One mechanism by which neonatal estrogen elicits teratogenic and carcinogenic effects is epigenetic and involves the modulation of a number of estrogen-regulated genes including epidermal growth factor (EGF). Because of the evidence that there is an integral relationship between the EGF family, estrogen action, and the regulation of the growth and differentiation of the reproductive tract, we used transforming growth factor-alpha (TGF alpha) transgenic mice to investigate the interaction of constitutive TGF alpha expression with the potent estrogen diethylstilbestrol (DES) in the induction of reproductive-tract alterations. Our study was designed to determine whether TGF alpha expression could modulate DES-induced carcinogenesis of the female mouse reproductive tract. The animals were homozygous TGF alpha transgenic female mice from the MT42 line and the parental CD-1 outbred mice. The presence of the TGF alpha transgene significantly increased the incidence of DES-induced vaginal adenosis, uterine endometrial hyperplasia, uterine polyps, hypospadia, benign ovarian cysts, and pituitary adenomas. However, constitutive TGF alpha expression did not promote reproductive-tract neoplasia. This study demonstrates that TGF alpha participates in the regulation of developmental and morphogenic events in the Müllerian duct and urogenital sinus, suggesting a role for TGF alpha in the pathogenesis of reproductive-tract diseases. Furthermore, we showed that although constitutive expression of the TGF alpha transgene did have an effect on the reproductive tract, TGF alpha overexpression alone could not substitute for DES as a reproductive-tract carcinogen or as a promoter of uterine neoplasia, indicating that DES-induced carcinogenesis requires events in addition to the overexpression of this single peptide growth factor.
Reliability study of micro-pin fin array for on-chip cooling (2015)

With continued power, performance, functionality, and miniaturization demands, microelectronic devices can dissipate upwards of 50 W/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> reaching to 100's of W/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> and possibly 1000 W/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> in the next 10 years. With such a high heat flux, traditional cooling through thermal interface material and heat spreader may not be able to remove the heat, especially from hot spots. In an ongoing research at Georgia Tech, innovative fluid-thermal solutions are being pursued where deionized water or refrigerants are circulated through an array of micro-pin fins etched into the backside of the silicon. In the proposed configuration, the thick backside of the silicon is used to etch an array of micro-pin fins and the silicon die is then bonded to another silicon or quartz substrate to create a micro-pin fin channel for fluid flow. To maximize heat flux, the fluid is expected to be in two-phase. The objective of this paper is to examine such a micro-pin fin array from the standpoint of cracking and reliability.
Ambit-Process based Channel Model for Urban Microcellular Communication at 140 GHz (2023)

&lt;p&gt;The design and development of sub-Terahertz (THz) communication systems entail the need for new channel models that can precisely predict channel characteristics at such frequencies (&gt;100 GHz) in outdoor and dynamic environments. This work proposes a novel hybrid-stochastic ultra-wideband channel model for sub-THz bands, developed within the framework of a class of spatio-temporal stochastic processes called ambit-process. The proposed model is capable of supporting bandwidths of upto 10 GHz. The spatio-temporal evolution of the ambit framework allows for a reasonably accurate and tractable characterization of the fading statistics and multipath propagation of a cellular channel with good spatial consistency. We leverage a recently proposed low-complexity ambit-process based channel simulation algorithm with necessary modifications to study key features of the outdoor sub-THz channel like associated diffused scattering, molecular absorption, spatio-temporal correlations, and consistency between the time-evolving delay and Doppler of the multipaths. Simulation results on path loss, delay spread, and channel correlation indicate that the ambit model accurately captures the typicalities of an urban microcellular sub-THz channel and agrees well with the measurement results reported in the literature. &lt;/p&gt;
SARS-CoV-2 can infect human embryos (2022)

The spread of SARS-CoV-2 has led to a devastating pandemic, with infections resulting in a range of symptoms collectively known as COVID-19. The full repertoire of human tissues and organs susceptible to infection is an area of active investigation, and some studies have implicated the reproductive system. The effects of COVID-19 on human reproduction remain poorly understood, and particularly the impact on early embryogenesis and establishment of a pregnancy are not known. In this work, we explore the susceptibility of early human embryos to SARS-CoV-2 infection. By using RNA-seq and immunofluorescence, we note that ACE2 and TMPRSS2, two canonical cell entry factors for SARS-CoV-2, are co-expressed in cells of the trophectoderm in blastocyst-stage preimplantation embryos. For the purpose of viral entry studies, we used fluorescent reporter virions pseudotyped with Spike (S) glycoprotein from SARS-CoV-2, and we observe robust infection of trophectoderm cells. This permissiveness could be attenuated with blocking antibodies targeting S or ACE2. When exposing human blastocysts to the live, fully infectious SARS-CoV-2, we detected cases of infection that compromised embryo health. Therefore, we identify a new human target tissue for SARS-CoV-2 with potential medical implications for reproductive health during the COVID-19 pandemic and its aftermath.
Glucocorticoid-remediable aldosteronism (GRA): Diagnosis, variability of phenotype and regulation of potassium homeostasis (1995)

Glucocorticoid-remediable aldosteronism (GRA) is a hereditary cause of human hypertension in which aldosterone secretion is regulated by adrenocorticotropin (ACTH). A genetic mutation which causes GRA has recently been identified in our laboratory, a hybrid or chimeric gene fusing nucleotide sequences of the 11 beta-hydroxylase and aldosterone synthase genes. The finding that these chimeric gene duplications are sensitive and specific markers for GRA allows for a simple, direct genetic test for this disorder. In preliminary studies, we found a wide range of blood pressure levels (including normotension) in affected GRA subjects. Studies to data indicate that this is not related to environmental factors such as sodium intake. Another possibility is that chimeric gene expression is variable, with low blood pressure subjects having reduced gene expression. However, the data have not demonstrated differences in steroid levels in subjects with severe versus mild hypertension. In fact, it is likely that the wide range in blood pressure levels in affected subjects involves interaction of other systems which control blood pressure. Preliminary data in two kindreds suggest that blood pressure levels are reciprocally related to levels of urinary kallikrein excretion, supporting the notion that GRA is a hypertension-predisposing syndrome, with the resultant blood pressure the interaction of the gene mutation with other blood pressure regulatory systems. Although GRA is a mineralocorticoid excess state, as evidenced by profoundly suppressed levels of plasma renin activity, we have observed (contrary to the reported literature) that normokalemia is a typical finding. In one large normokalemic pedigree, preliminary findings indicate that these subjects have a normal capacity to excrete potassium.(ABSTRACT TRUNCATED AT 250 WORDS)