gebhart/patrepeval huggingface dataset:
Role-based privilege and trust management (2005)

Abstract. The Internet provides tremendous connectivity and information sharing capability which organizations can use for their competitive advantage. However, we still observe security challenges in Internet-based applications, especially in terms of their limited support for controlled access to organizational resources and information for unknown users. Roles can be a convenient construct for expressing entitled privileges and trust degree alike, based upon which further specification of responsibility and capability is made so as to facilitate trust-based authorization for such an environment. In this article, we design a role-based privilege and trust management by leveraging a role-based trust model and a privilege management infrastructure, as an attempt to develop an easyto-use, flexible, and interoperable authorization mechanism for unknown users. Also, we demonstrate the feasibility of our mechanism by providing a proof-of-concept prototype implementation using commercial off-the-shelf technologies. 1
Semi-insulating 6H–SiC grown by physical vapor transport (1995)

Semi-insulating 6H–SiC crystals have been achieved by using controlled doping with deep-level vanadium impurities. High resistivity undoped and semi-insulating vanadium-doped single-crystals with diameters up to 50 mm were grown by physical vapor transport using an induction-heated, cold-wall system in which high purity graphite materials constituted the hot zone of the furnace. Undoped crystals were p-type due to the presence of residual acceptor impurities, mainly boron, and exhibited resistivities ranging up to 3000 Ω cm. The semi-insulating behavior of the vanadium-doped crystals is attributed to compensation of residual acceptors by the deep-level vanadium V4+(3d1) donor located near the middle of the band gap.
New kernels for fast mesh-based motion estimation (2001)

Mesh-based motion estimation-also known as control grid interpolation or warping-provides a smoother estimated intensity field compared to the traditional block-matching algorithm (BMA), resulting in most cases in a more realistic motion field and smaller estimation error. In mesh-based motion, unlike BMA, the computation of a motion vector is affected by its neighboring vectors. This interdependence necessitates a costly, iterative computation of motion vectors. The computational cost of mesh-based motion has been a main drawback of this otherwise powerful technique. We propose to use noniteratively computed motion vectors, such as BMA motion vectors, for node motions in the mesh model. However, we found that a straightforward insertion of BMA motion vectors in the deformable mesh leads to unpredictable and erratic results, and were thus motivated to carefully analyze the interaction of motion vectors and interpolation kernels in mesh models. This analysis leads to a methodology for computing optimal motion interpolation kernels for a given set of motion vectors (e.g., BMA motion vectors). We find a generalized orthogonality condition for these kernels; optimality is achieved only if the projections of vertex motions on the local intensity gradients are statistically orthogonal to mesh-based estimation errors. Experiments show that optimal kernels are often very different from the traditional bilinear kernels, and exhibit interesting variations. The new kernels benefit a variety of applications, including motion estimated interpolation, denoising, and compression.
Growth of compressive strained in GaAs/GaAs multiple quantum well structures by MBE (1997)

Indian Journal of Pure and Applied Physics
Comparison of the binding pockets of two chemically unrelated allosteric antagonists of the mGlu5 receptor and identification of crucial residues involved in the inverse agonism of MPEP (2006)

Fenobam [N-(3-chlorophenyl)-N'-(4,5-dihydro-1-methyl-4-oxo-1H-imidazole-2-yl)urea], a clinically validated non-benzodiazepine anxiolytic, has been shown to be a potent and non-competitive metabotropic glutamate (mGlu)-5 receptor antagonist. In the present study, we have used the site-directed mutagenesis coupled with three-dimensional receptor-based pharmacophore modelling to elucidate the interacting mode of fenobam within the seven-transmembrane domain (7TMD) of mGlu5 receptor and its comparison with that of 2-methyl-6-(phenylethynyl)pyridine (MPEP), the prototype antagonist. The common residues involved in the recognition of MPEP and fenobam include Pro654(3.36), Tyr658(3.40), Thr780(6.44), Trp784(6.48), Phe787(6.51), Tyr791(6.55) and Ala809(7.47). The differentiating residues between both modulators' interacting modes are Arg647(3.29), Ser657(3.39) and Leu743(5.47). Our data suggest that these chemically unrelated mGlu5 antagonists act similarly, probing a functionally unique region of the 7TMD. Using [3H]inositol phosphates accumulation assay, we have also identified the critical residues involved in the inverse agonist effect of MPEP. The mutation W784(6.48)A completely blocked the inverse agonist activity of MPEP; two mutations F787(6.51)A and Y791(6.55)A, caused a drastic decrease in the MPEP inverse agonism. Furthermore, these three mutations led to an increased efficacy of quisqualate without having any effect on its potency. The fact that the residues Trp784(6.48) and Phe787(6.51) are essential equally in antagonism and inverse agonism effects emphasizes again the key role of these residues and the involvement of a common transmembrane network in receptor inactivation by MPEP.
A Novel Functional Site in the PB2 Subunit of Influenza A Virus Essential for Acetyl-CoA Interaction, RNA Polymerase Activity, and Viral Replication (2014)

The PA, PB1, and PB2 subunits, components of the RNA-dependent RNA polymerase of influenza A virus, are essential for viral transcription and replication. The PB2 subunit binds to the host RNA cap (7-methylguanosine triphosphate (m(7)GTP)) and supports the endonuclease activity of PA to "snatch" the cap from host pre-mRNAs. However, the structure of PB2 is not fully understood, and the functional sites remain unknown. In this study, we describe a novel Val/Arg/Gly (VRG) site in the PB2 cap-binding domain, which is involved in interaction with acetyl-CoA found in eukaryotic histone acetyltransferases (HATs). In vitro experiments revealed that the recombinant PB2 cap-binding domain that includes the VRG site interacts with acetyl-CoA; moreover, it was found that this interaction could be blocked by CoA and various HAT inhibitors. Interestingly, m(7)GTP also inhibited this interaction, suggesting that the same active pocket is capable of interacting with acetyl-CoA and m(7)GTP. To elucidate the importance of the VRG site on PB2 function and viral replication, we constructed a PB2 recombinant protein and recombinant viruses including several patterns of amino acid mutations in the VRG site. Substitutions of the valine and arginine residues or of all 3 residues of the VRG site to alanine significantly reduced the binding ability of PB2 to acetyl-CoA and its RNA polymerase activity. Recombinant viruses containing the same mutations could not be replicated in cultured cells. These results indicate that the PB2 VRG sequence is a functional site that is essential for acetyl-CoA interaction, RNA polymerase activity, and viral replication.
High-resolution cDNA microarray CGH mapping of genomic imbalances in osteosarcoma using formalin-fixed paraffin-embedded tissue (2004)

Formalin-fixed paraffin embedded (FFPE) tumor tissue provides an opportunity to perform retrospective genomic studies of tumors in which chromosomal imbalances are strongly associated with oncogenesis. The application of comparative genomic hybridization (CGH) has led to the rapid accumulation of cytogenetic information on osteosarcoma (OS); however, the limited resolving power of metaphase CGH does not permit precise mapping of imbalances. Array CGH allows quantitative detection and more precise delineation of copy number aberrations in tumors. Unfortunately the high cost and lower density of BACs on available commercial arrays has limited the ability to comprehensively profile copy number changes in tumors such as OS that are recurrently subject to genomic imbalance. In this study a cDNA/EST microarray including 18,980 human cDNAs (which represent all 22 pairs of autosomal chromosomes and chromosome X) was used for CGH analysis of eight OS FFPE. Chromosomes 1, 12, 17, and X harbored the most imbalances. Gain/amplification of X was observed in 4/8 OS, and in keeping with other recent genomic analyses of OS, gain/amplification of 17p11.2 was often accompanied by a distal deletion in the region of the p53 gene. Gain/amplification of the X chromosome was verified using interphase FISH carried out on a subset of OS FFPE sections and OS tissue arrays.
Scattering-rate approach for efficient prediction of temperature-dependent characteristics of mid-infrared quantum cascade lasers (2019)

A computationally efficient temperature-dependent model for scattering processes in mid-infrared quantum cascade lasers is developed. In this approach, the total intersubband scattering rate is described as the product of the overlap integral for the squared moduli of the envelope functions and a form factor that depends on transition energy, temperature, and material. Both inelastic and elastic scattering processes are included in the treatment. The model is used to calculate the temperature-dependent threshold current density $ {J_{{\rm th}}}(T) $Jth(T) in a 10-period strain-compensated InGaAs–InAlAs mid-infrared quantum cascade laser structure and determine the characteristic temperature $ {T_0} $T0 in $ {J_{{\rm th}}}(T) = {J_0}\exp {(T/{T_0})} $Jth(T)=J0exp⁡(T/T0). The effect of doping is also modeled.
Distribution of galanin‐like immunoreactivity in the brain of Rana esculenta and Xenopus laevis (1991)

The immunocytochemical distribution of galanin-containing perikarya and nerve terminals in the brain of Rana esculenta and Xenopus laevis was determined with antisera directed toward either porcine or rat galanin. The pattern of galanin-like immunoreactivity appeared to be identical with antisera directed toward either target antigen. The distribution of galanin-like immunoreactivity was similar in Rana esculenta and Xenopus laevis except for the absence of a distinct laminar distribution of immunoreactivity in the optic tectum of Xenopus laevis. Galanin-containing perikarya were located in all major subdivisions of the brain except the metencephalon. In the telencephalon, immunoreactive perikarya were detected in the pars medialis of the amygdala and the preoptic area. In the diencephalon, immunoreactive perikarya were detected in the caudal half of the suprachiasmatic nucleus, the nucleus of the periventricular organ, the ventral hypothalamus, and the median eminence. In the mesencephalon, immunoreactive perikarya were detected near the midline of the rostroventral tegmentum, in the torus semicircularis and, occasionally, in lamina A and layer 6 of the optic tectum. In the myelencephalon, labelled perikarya were detected only in the caudal half of the nucleus of the solitary tract. Immunoreactive nerve fibers of varying density were observed in all subdivisions of the brain with the densest accumulations of fibers occurring in the pars lateralis of the amygdala and the preoptic area. Dense accumulations of nerve fibers were also found in the lateral septum, the medial forebrain bundle, the periventricular region of the diencephalon, the ventral hypothalamus, the median eminence, the mesencephalic central gray, the laminar nucleus of the torus semicircularis, several laminae of the optic tectum, the interpeduncular nucleus, the isthmic nucleus, the central gray of the rhombencephalon, and the dorsolateral caudal medulla. The extensive system of galanin-containing perikarya and nerve fibers in the brain of representatives of two families of anurans showed many similarities to the distribution of galanin-containing perikarya and nerve fibers previously described for the mammalian brain.
Bistable Operation of a Two-Core Coherently Combined Fiber Laser (2017)

Multiple stable supermodes are predicted and observed in a Y-shaped resonator. Applied phase error between the two gain arms is passively compensated by self-adjustment of the gain-dependent phase. Observation of hysteresis in self-phasing confirms bistability.
Cancer Diagnosis in Patients with Heart Failure: Epidemiology, Clinical Implications and Gaps in Knowledge (2018)

Cancer and heart failure (HF) are common medical conditions with a steadily rising prevalence in industrialized countries, particularly in the elderly, and they both potentially carry a poor prognosis. A new diagnosis of malignancy in subjects with pre-existing HF is not infrequent, and challenges HF specialists as well as oncologists with complex questions relating to both HF and cancer management. An increased incidence of cancer in patients with established HF has also been suggested. This review paper summarizes the epidemiology and the prognostic implications of cancer occurrence in HF, the impact of pre-existing HF on cancer treatment decisions and the impact of cancer on HF therapeutic options, while providing some practical suggestions regarding patient care and highlighting gaps in knowledge.
The Influence of Homogeneous Reactions on the Impedance Response of a Rotating Disk Electrode (2017)

This paper is part of the JES Focus Issue on Mathematical Modeling of Electrochemical Systems at Multiple Scales in Honor of John Newman
Dopamine Metabolite Biomarkers and Testing for Disease Modification in Parkinson Disease—Reply (2020)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue
Naegleria fowleri: Protein structures to facilitate drug discovery for the deadly, pathogenic free-living amoeba (2021)

Naegleria fowleri is a pathogenic, thermophilic, free-living amoeba which causes primary amebic meningoencephalitis (PAM). Penetrating the olfactory mucosa, the brain-eating amoeba travels along the olfactory nerves, burrowing through the cribriform plate to its destination: the brain's frontal lobes. The amoeba thrives in warm, freshwater environments, with peak infection rates in the summer months and has a mortality rate of approximately 97%. A major contributor to the pathogen's high mortality is the lack of sensitivity of N. fowleri to current drug therapies, even in the face of combination-drug therapy. To enable rational drug discovery and design efforts we have pursued protein production and crystallography-based structure determination efforts for likely drug targets from N. fowleri. The genes were selected if they had homology to drug targets listed in Drug Bank or were nominated by primary investigators engaged in N. fowleri research. In 2017, 178 N. fowleri protein targets were queued to the Seattle Structural Genomics Center of Infectious Disease (SSGCID) pipeline, and to date 89 soluble recombinant proteins and 19 unique target structures have been produced. Many of the new protein structures are potential drug targets and contain structural differences compared to their human homologs, which could allow for the development of pathogen-specific inhibitors. Five of the structures were analyzed in more detail, and four of five show promise that selective inhibitors of the active site could be found. The 19 solved crystal structures build a foundation for future work in combating this devastating disease by encouraging further investigation to stimulate drug discovery for this neglected pathogen.
Case 1: Poor Growth With Presence of a Pituitary Lesion in an 11-year-old Boy (2017)

1. Alexander S. Karageorgiadis, MD*,†
2. Charalampos Lyssikatos, MD*
3. Elena Belyavskaya, MD*
4. Georgios Z. Papadakis, MD‡
5. Nicholas J. Patronas, MD‡
6. Maya B. Lodish, MD*
7. Constantine A. Stratakis, MD, DSc*


 

1. *Section on Endocrinology and Genetics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD

2. †Department of Pediatrics, Georgetown University Hospital, Washington, DC

3. ‡Department of Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, Bethesda, MD

An 11-year-old boy presents to his primary pediatrician due to poor growth. His height was at the 25th percentile between ages 6 and 10 years and now has fallen to the 10th percentile. He also complains of fatigue and has a 1-month history of polyuria, polydipsia, and nocturia. Review of symptoms is negative for weight loss, headaches, vision changes, gynecomastia, breast discharge, nausea, vomiting, and cold and heat intolerance. His past medical and family histories are not contributory and his development has been normal.

On physical examination, his temperature is 96.8°F (36oC), blood pressure is 86/52 mm Hg, heart rate is 59 beats/min, respiratory rate is 18 breaths/min, and oxygen saturation is 100% in room air. His standing height is 138.23 cm (10th percentile), weight is 31.5 kg (10th percentile), and body mass index is 16.5 (10th-50th percentile). His neurologic examination yields unremarkable results. His pubic hair is at Sexual Maturity Rating stage 2 and testicular volume of both testes is 10 mL.

Laboratory evaluation shows free thyroxine (T4) of 0.76 ng/dL (9.78 pmol/L) (normal range 0.93-1.60 ng/dL [11.97-20.59 pmol/L]), total T4 of 4.3 μg/dL (55.35 nmol/L) (normal range 4.5-12 μg/dL [57.92-154.45 nmol/L]), thyrotropin (TSH) of 1.97 mIU/L (normal range 0.45-4.5 mIU/L), and insulinlike growth factor (IGF-1) of 103 ng/mL (13.49 nmol/L) (normal range for …
Interobject spacing control and controllability of a manufacturing transportation system (1998)

Presents a control and controllability approach for a general manufacturing transportation system. The system transports objects from a production unit to a stamping/packaging section. At arrival time, the position and velocity of every object must synchronize with the stamping device. The system constraints and a simplified model of the system dynamics are developed. The hybrid character of the system makes classical control approaches difficult. A control scheme that controls interobject spacings and satisfies the constraints is proposed. A controllability analysis, independent from the control scheme and satisfying all constraints, shows the influence of system parameters on system controllability. Simulations verify the control scheme and controllability analysis.
The living cell supply chain: a product-specific, rational approach to management of cellular starting material donors & donations (2022)

William E Janssen, WEJ Cell and Gene Therapy Consulting Services, &amp; Scott R Burger, Advanced Cell and Gene Therapy, LLC“In cell therapy and cell-based gene therapy products,donated, intact living cells are arguably the most criticalof materials for CGT product manufacturing”
Structures of Withaperuvin B and C, Withanolides of Physalis peruviana Roots (1982)

Two minor withanolides, withaperuvin B and C, have been isolated from the roots of Physalis peruviana.Their structures have been elucidated as those represented by formulae 1 and 2, respectively, based on chemical and spectral evidence.In a previous publication,2 the structure of withaperuvin, the major steroid of the roots of Physalis peruviana Limn6 (Solanaceae), was settled as 3 on the basis of spectral evidence and X-ray analysis of the derived androstane derivative (4).The present cooolunication describes the structure determination of withaperuvin 8 and C, the two minor steroidal constituents ieolated from the same plant source.Withaperuvin B, m,p.269-270•‹, [alD + 2 1 5 * (pyridine), w a s Found to possess molecular weight 502 (FD-MS) which,
Analysis and Full Scale Crash Test of a High Containment Bridge Rail (2012)

Bridge rails are built to safely redirect errant vehicles so as to protect occupants and without excessively damaging the bridge rail structures. Criteria to evaluate performance of bridge rails are described in details in working standards like the National Cooperative Highway Research Program Report 350 “Recommended Procedures for the Safety Performance Evaluation of Highway Features” and European Norm (EN) 1317. In this paper, predictive finite element analyses and subsequent full scale crash tests are presented for a high containment bridge rail design. The bridge rail design was analyzed using nonlinear finite element method using the conditions of EN-1317 tests TB-11 and TB-71. EN-1317 test TB-11 involves a 900 kg passenger car impacting the barrier at a speed of 100 km/h and an angle of 20 degrees. EN-1317 test TB-71 involves a 30,000 kg heavy goods vehicle (HGV) impacting the barrier at a speed of 65 km/h and an angle of 20 degrees. Based on the simulation, design changes were made to the shape of the barrier and starter reinforcement bars between the bridge deck and the rail. New analyses of the modified design were conducted and the results indicated a good likelihood of passing the EN-1317 test criteria. Subsequently, two full scale crash tests (EN-1317 tests TB-11 and TB-71) of the bridge rail system were conducted. The tests shows reasonable agreement with the finite element simulations and the rail system is considered passed per EN-1317 criteria for containment level H4a.
Figure S7 from Identification of Membrane-expressed CAPRIN-1 as a Novel and Universal Cancer Target, and Generation of a Therapeutic Anti-CAPRIN-1 Antibody TRK-950 (2023)

&lt;p&gt;Properties of TRK-950&lt;/p&gt;
10th Anniversary Anticipation (2010)

ADVERTISEMENT RETURN TO ISSUEEditorialNEXT10th Anniversary AnticipationRobin D. RogersCite this: Cryst. Growth Des. 2010, 10, 1, 1–2Publication Date (Web):January 6, 2010Publication History Received7 December 2009Published online6 January 2010Published inissue 6 January 2010https://doi.org/10.1021/cg901530pCopyright © 2010 American Chemical SocietyRIGHTS & PERMISSIONSArticle Views722Altmetric-Citations3LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (715 KB) Get e-AlertsSUBJECTS:Crystal engineering,Crystallization,Crystals,Pharmaceuticals Get e-Alerts
A cryptographic application of the Thurston norm (2020)

We discuss some applications of 3-manifold topology to cryptography. In particular, we propose a public-key and a symmetric-key cryptographic scheme based on the Thurston norm on the first cohomology of hyperbolic manifolds.