gebhart/patrepeval huggingface dataset:
Identification and characterization of soluble and insoluble myrosinase isoenzymes in different organs of Sinapis alba (2001)

Extraction of Sinapis alba seeds under native conditions solubilized 3 myrosinase isoforms, pool I, II and III, which could be separated by ion exchange chromatography. Sequencing of numerous peptides of the I and III isoforms showed that they belonged to the Myrosinase A (MA) family of myrosinases and that they were encoded by different genes. Western blot analysis of S. alba seed proteins, extracted with a sodium dodecyl sulphate-containing buffer, using an anti-myrosinase monoclonal antibody, showed the presence of two additional myrosinase isoforms with approximate molecular sizes of 62 and 59 kDa. These myrosinases, which only could be solubilized from seeds by inclusion of denaturing agents in the extraction buffer, were by sequence analysis identified as MB myrosinases. These isoenzymes or very similar forms were also present in seedling cotyledons. However, from this tissue, they could be extracted with non-denaturing buffers. In addition, cotyledons contained a 65-kDa MB myrosinase not found in seeds. In contrast, seedling cotyledons contained only minute amounts of pool I and no pool III MA myrosinases, emphasizing the tissue-specific expression of the corresponding gene families. Sequence analysis of myrosinase cDNAs generated cDNA by reversed transcription-polymerase chain reaction using degenerate primers with mRNA isolated from seeds, cotyledons and leaves confirmed the result that the MA isoforms were expressed only in seed tissue, while MB myrosinases were found in all tissues investigated. Furthermore, seed and leaf contained unique MB myrosinase transcripts, suggesting organ-specific expression of individual MB genes.
Quantum Superposition State Scattering in Ytterbium (1984)

Collisions of atoms in superpositions of dissimilar states have special significance in that, classically, each state follows a different trajectory. Hence the scattering of such a system as a unit has no classical analog and is of fundamental interest. We report studies of velocity-changing collision kernels in 174Yb for two types of superposition states: optical radiators, consisting of a ground S state and an excited P state, and P-state multipole moments, involving magnetic substate superpositions.
Synthesis and Application of New Ionic Liquids with Tris(perfluoroalkyl)trifluorophosphate Anions (2007)

The synthesis of new ionic liquids with tris(perfluoroalkyl)-trifluorophosphate (FAP) anions is described. The physico-chemical properties of this new generation of ionic liquids are discussed. FAP ionic liquids show an excellent hydrolytic stability, low viscosity and high thermal stability, that makes them attractive for the application as new reaction media in organic synthesis.
Control of coffee grinding with Artificial Neural Networks (2012)

Quality assessment and standardization of the property of the final product is fundamental in food industry. Coffee particle granulometry and density are continuously monitored during coffee beans grinding and grinders are controlled by operators in order to keep coffee particle granulometry within specific thresholds. In this work, a neural system is used to learn how to control two grinders used for coffee production at LAVAZZA factory, obtaining average control error of the order of a few μm. The results appear promising for the future development of an automatic decision support system.
Lumped-element Josephson parametric amplifier at 650 MHz for nano-calorimeter readout (2017)

We design a sub-gigahertz Josephson parametric amplifier for the readout of nanoscale calorimeters which consist of normal-metal–superconductor heterostructures. We characterize the amplifier performance at two operating points, 605 and 655 MHz, corresponding to reproducible local frequency maxima with respect to the applied magnetic flux. At the 655 MHz operating point, the device displays its maximum small-signal gain of 32 dB and gain-bandwidth product of . The gain remains above 20 dB for incident powers up to −119 dBm. The added noise of the amplifier, determined by the hot/cold source method, assumes a minimum value of 0.2 K.
Modeling and instrumentation for fault detection and isolation of a cooling system (2002)

Functional redundancy techniques for fault detection and isolation (FDI) in dynamic systems requires close interaction between system instrumentation, modeling and analysis. Effective FDI requires detailed and accurate models to trade and analyze system behavior, including transient phenomena that result from faults. It also requires appropriate instrumentation technology to provide the measurements to capture and analyze system behavior. Models and measurements must be matched carefully to provide sufficient observability and effective analysis. In this paper we demonstrate the development of FDI systems for complex applications by presenting the modeling and instrumentation of an automobile combustion engine cooling system. We have developed a qualitative parameter estimation methodology for FDI. A system model is represented as a graph that captures the dynamic behavior of the system. To demonstrate the applicability a small leak is artificially introduced in the cooling system and accurately detected and isolated.
Position statement: nonoperative management of lateral epicondylitis in adults (2022)

We sought to compare methods of nonsurgical treatment of lateral epicondylitis in men and women older than 18 years to develop a guideline intended for orthopedic surgeons and other health care providers who assess, counsel and care for these patients. We searched Medline, Embase and Cochrane through to Mar. 9, 2021, and included all English-language studies comparing nonsurgical approaches. We compared physiotherapy versus no active treatment, corticosteroids versus placebo, platelet-rich plasma (PRP) versus placebo, and autologous blood injection versus placebo. Outcomes of interest were pain outcomes (visual analogue scale scores) and functional outcomes. We rated the quality of the evidence and strength of recommendations using the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) approach. This guideline will benefit patients seeking nonsurgical intervention for lateral epicondylitis by improving counselling on nonsurgical treatment options and possible outcomes. It will also benefit surgical providers by improving their knowledge of various nonsurgical approaches. Data presented could be used to develop frameworks and tools for shared decision-making.
Long-term outcomes after severe drug overdose (2016)

ICU and general ward patients with overdose have similar, overwhelming prevalences of psychiatric disease, and similar outcome profiles. Such patients experience frequent overdoses and, despite being young, if admitted to the ICU, have a 10% 4-year mortality, with self-harm the dominant cause of death. Finally, among survivors who responded to the follow-up questionnaire, quality of life is poor.
Rat group I Metabotropic Glutamate Receptors Inhibit Neuronal Ca2+Channels via Multiple Signal Transduction Pathways in HEK 293 Cells (1998)

We have shown previously that metabotropic glutamate receptors with group I-like pharmacology couple to N-type and P/Q-type calcium channels in acutely isolated cortical neurons using G proteins most likely belonging to the Gi/Go subclass. To better understand the potential mechanisms forming the basis for group I mGluR modulation of voltage-gated calcium channels in the CNS, we have examined the ability of specific mGluRs to couple to neuronal N-type (alpha1B-1/alpha2delta/beta1b) and P/Q-type (alpha1A-2/alpha2delta/beta1b) voltage-gated calcium channels in an HEK 293 heterologous expression system. Using the whole cell patch-clamp technique where intracellular calcium is buffered to low levels, we have shown that group I receptors inhibit both N-type and P/Q-type calcium channels in a voltage-dependent fashion. Similar to our observations in cortical neurons, this voltage-dependent inhibition is mediated almost entirely by N-ethylmaleimide (NEM)-sensitive heterotrimeric G proteins, strongly suggesting that these receptors can use Gi/Go-like G proteins to couple to N-type and P/Q-type calcium channels. However, inconsistent with the apparent NEM sensitivity of group I modulation of calcium channels, modulation of N-type channels in group I mGluR-expressing cells was only partially sensitive to pertussis toxin (PTX), indicating the potential involvement of both PTX-sensitive and -resistant G proteins. The PTX-resistant modulation was voltage dependent and entirely resistant to NEM and cholera toxin. A time course of treatment with PTX revealed that this toxin caused group I receptors to slowly shift from using a primarily NEM-sensitive G protein to using a NEM-resistant form. The PTX-induced switch from NEM-sensitive to -resistant modulation was also dependent on protein synthesis, indicating some reliance on active cellular processes. In addition to these voltage-dependent pathways, perforated patch recordings on group I mGluR-expressing cells indicate that another slowly developing, calcium-dependent form of modulation for N-type channels may be seen when intracellular calcium is not highly buffered. We conclude that group I mGluRs can modulate neuronal Ca2+ channels using a variety of signal transduction pathways and propose that the relative contributions of different pathways may exemplify the diversity of responses mediated by these receptors in the CNS.
Plasmid transfer and expression of the transfer (tra) gene product of plasmid pIJ101 are temporally regulated during the Streptomyces lividans life cycle (1996)

We previously have shown that a chromosomally integrated copy of the tra gene of plasmid pIJ101 under the control of the KorA repressor protein, which regulates transcription of tra as well as its own synthesis, can promote the conjugal transfer of both chromosomal and plasmid genes in Streptomyces lividans. Using an antibody generated against a fusion protein containing the C-terminal portion of Tra, we show here that this essential conjugation protein is present in membrane fractions of both surface-grown S. lividans, which mate readily, and of cells grown in liquid culture, where mating has not been found. Expression of Tra during the S. lividans life cycle was temporally regulated and was reduced late during vegetative growth so that little or no Tra protein was detected in cells as they began to differentiate morphologically and produce secondary metabolites. Comparison of the membrane concentration of Tra protein with tra mRNA concentration during the S. lividans life cycle indicated that the disappearance of Tra is post-transcriptionally controlled and thus is not mediated by KorA. The results of 'interrupted mating' experiments, together with the time of appearance of Tra in S. lividans membranes, indicate that the intermycelial transfer of pIJ101 in S. lividans is complete by the onset of cellular differentiation.
Adaptive optics allows 3D STED-FCS measurements in the cytoplasm of living cells (2019)

Fluorescence correlation spectroscopy in combination with super-resolution stimulated emission depletion microscopy (STED-FCS) is a powerful tool to investigate molecular diffusion with sub-diffraction resolution. It has been of particular use for investigations of two dimensional systems like cell membranes, but has so far seen very limited applications to studies of three-dimensional diffusion. One reason for this is the extreme sensitivity of the axial (3D) STED depletion pattern to optical aberrations. We present here an adaptive optics-based correction method that compensates for these aberrations and allows STED-FCS measurements in the cytoplasm of living cells.
PKC412 Inhibits In Vitro Growth of Neoplastic Mast Cells Expressing the D816V-Mutated Variant of KIT: Comparison with AMN107 and Imatinib, and Evaluation of Drug-Interactions. (2005)

Abstract In most patients with systemic mastocytosis (SM) including aggressive SM and mast cell leukemia (MCL), neoplastic cells express the oncogenic c-KIT mutation D816V. KIT-D816V is associated with constitutive tyrosine kinase (TK) activity and thus represents an attractive target of drug therapy. However, most available TK inhibitors including STI571=imatinib, fail to block TK-activity of KIT D816V at pharmacologic concentrations. We provide evidence that the novel TK-targeting drugs PKC412 and AMN107 decrease TK-activity of D816V-mutated KIT and counteract growth of Ba/F3 cells with doxycycline-induced expression of KIT D816V as well as growth of the human mast cell leukemia cell line HMC-1 expressing this c-KIT mutation. PKC412 was found to be the superior drug with IC50 values of 50–250 nM and without differences seen between HMC-1 cells exhibiting or lacking KIT D816V. By contrast, AMN107 exhibited potent effects only in the absence of KIT D816V in HMC-1 cells. Corresponding results were obtained with Ba/F3 cells exhibiting wild-type or the D816V-mutated variant of KIT. Moreover, we found that PKC412 and AMN107 inhibit growth of primary neoplastic MC in a patient with KIT D816V+ SM. The growth-inhibitory effects of PKC412 and AMN107 on HMC-1 cells were associated with TK-inhibition of KIT and with induction of apoptosis. In addition, PKC412 was found to downregulate expression of CD2 and CD63, two cell surface antigens upregulated in SM. In co-incubation experiments, PKC412 was found to synergize with AMN107, imatinib, and 2CdA in producing growth inhibition in HMC-1 cells lacking KIT D816V, whereas in KIT D816V+ HMC-1 cells, drug-interactions were additive rather than synergistic. Together, PKC412 and AMN107 alone and in combination counteract growth of neoplastic mast cells. Both drugs may therefore be considered as novel promising agents for targeted therapy in patients with aggressive SM or MCL.
100 The Impact of Dietary Analyzed Ca to P Ratios and Standardized Total Tract Digestible P to Net Energy Ratios on Growth Performance, Bone, and Carcass Characteristics of Pigs (2022)

Abstract A total of 2,184 pigs (initially 12.4 kg) were used to evaluate effects of varying analyzed Ca:P ratios at 2 levels of standardized total tract digestible (STTD) P:NE. Pens of pigs (26 pigs/pen) were assigned to 6 dietary treatments in a randomized complete block design with 14 pens/treatment. Diets consisted of 2 levels of STTD P:NE: PIC recommendation (1.8, 1.62, 1.43, 1.25, 1.10, 0.99 g STTD P/Mcal NE from 11-22, 22-40, 40-58, 58-81, 81-104, 104-129 kg, respectively) or 75% of the PIC recommendation, and 3 analyzed Ca:P ratios: 0.90:1, 1.30:1, and 1.75:1. Diets were corn-soybean meal-based and contained phytase (Quantum Blue G, AB Vista, Marlborough, UK; 500 to 210 FTU/kg with release values from 0.13 to 0.07% STTD P). There was a Ca:P×STTD P:NE interaction (P &amp;lt; 0.05) observed for ADG, G:F and final BW. For ADG and final BW, when feeding 75% of PIC STTD P recommendation, increasing analyzed Ca:P ratio decreased ADG and final BW (linear, P&amp;lt; 0.001). But when feeding at PIC STTD P recommendation, increasing the analyzed Ca:P ratio tended to improve ADG and final BW (linear, P&amp;lt; 0.10). For G:F, when feeding 75% of PIC STTD P recommendation, increasing analyzed Ca:P ratio tended to decrease G:F (linear, P &amp;lt; 0.10), whereas in pigs fed diets that met PIC STTD P recommendations, increasing analyzed Ca:P ratio tended to improve G:F in a quadratic (P &amp;lt; 0.10) manner. Pigs fed at PIC STTD P recommendations had increased ADG, final BW, and G:F compared with pigs fed 75% of PIC STTD P recommendations (P &amp;lt; 0.001). In summary, pigs fed at PIC STTD P recommendations had improved overall ADG and G:F compared with pigs fed diets at 75% PIC STTD P recommendations. Additionally, increasing analyzed Ca:P ratio reduced ADG and G:F when STTD P was below PIC recommendations, but had marginal impacts when adequate STTD P was fed.
Underlayer Designs to Enhance EUV Resist Performance (2009)

Extreme ultraviolet (EUV) lithography has gained momentum as the method of choice for <32-nm half-pitch device fabrication. In this paper, we describe our initial efforts to increase the EUV resist's performance via introduction of a thermally crosslinkable underlayer. We have demonstrated the benefits of adding an EUV underlayer into the regular EUV litho stack and investigated the effect of underlayer film thickness, post-coat bake temperature, and adding other additives such as PAG and sensitizer on the overall litho performance of EUV resists.
Networking for critical conditions (2008)

To enhance the preparedness of federal and state agencies to effectively manage federal or state recovery efforts in response to a broad spectrum of emergencies, we propose a hybrid adaptive network that will adopt currently available off-the-shelf wireless network devices and integrate them quickly into a scalable, reliable, and secure network with a minimum of human intervention for configuration and management. This model will serve as the framework for various rescue missions for securing and distributing critical resources. We investigate different technologies and network strategies and integrate them into the proposed network model to provide seamless support to heterogeneous environments including wireline nodes, ad hoc and sensor network nodes, and network devices based on different standards. In this article we present the network architecture and identify the key technical aspects of its management, security, QoS, and implementation.
Functionalized phenylimidazole-based facial-homoleptic iridium(iii) complexes and their excellent performance in blue phosphorescent organic light-emitting diodes (2018)

Novel phenylimidazole-based blue-emitting iridium complexes were prepared and they showed the excellent electroluminescent performance.
Drugs from deadly doses (1998)

The search for new therapeutic agents is gaining momentum, with snake venoms being just one of the pharmaceutical industry's targets. Researchers are using molecular methods to identify the bioactive molecules in Australian snake venoms.
Generalized temporal transfer matrix method: a systematic approach to solving electromagnetic wave scattering in temporally stratified structures (2022)

Opening a new door to tailoring electromagnetic (EM) waves, temporal boundaries have attracted the attention of researchers in recent years, which have led to many intriguing applications. However, the current theoretical approaches are far from enough to handle the complicated temporal systems. In this paper, we develop universal matrix formalism, paired with a unique coordinate transformation technique. The approach can effectively deal with temporally stratified structures with complicated material anisotropy and arbitrary incidence angles. This formulation is applied to various practical systems, enabling the solution of these temporal boundary related problems in a simple and elegant fashion, and also facilitating a deep insight into the fundamental physics.
Subclinical Interstitial Lung Disease: Why You Should Care (2012)

The widespread use of high-resolution computed tomography in clinical and research settings has increased the detection of interstitial lung abnormalities (ILA) in asymptomatic and undiagnosed individuals. We reported that in smokers, ILA were present in about 1 of every 12 high-resolution computed tomographic scans; however, the long-term significance of these subclinical changes remains unclear. Studies in families affected with pulmonary fibrosis, smokers with chronic obstructive pulmonary disease, and patients with inflammatory lung disease have shown that asymptomatic and undiagnosed individuals with ILA have reductions in lung volume, functional limitations, increased pulmonary symptoms, histopathologic changes, and molecular profiles similar to those observed in patients with clinically significant interstitial lung disease (ILD). These findings suggest that, in select at-risk populations, ILA may represent early stages of pulmonary fibrosis or subclinical ILD. The growing interest surrounding this topic is motivated by our poor understanding of the inciting events and natural history of ILD, coupled with a lack of effective therapies. In this perspective, we outline past and current research focused on validating radiologic, physiological, and molecular methods to detect subclinical ILD. We discuss the limitations of the available cross-sectional studies and the need for future longitudinal studies to determine the prognostic and therapeutic implications of subclinical ILD in populations at risk of developing clinically significant ILD.
ARIA 2016: Integrated care pathways for predictive medicine across the life cycle (2017)

The Allergic Rhinitis and its Impact on Asthma (ARIA) initiative commenced during a World Health Organization (WHO) workshop in 1999. The initial goals were to propose a new allergic rhinitis classification, to promote the concept of multi-morbidity in asthma and rhinitis and to develop guidelines with all stakeholders for global use in all countries and populations. ARIA - disseminated and implemented in over 70 countries globally - is now focusing on the implementation of emerging technologies for individualized and predictive medicine. MASK [MACVIA (Contre les MAladies Chroniques pour un Vieillissement Actif) - ARIA Sentinel NetworK] uses mobile technology to develop care pathways in order to enable the management of rhinitis and asthma by a multi-disciplinary group or by patients themselves. An App (Android and iOS) is available in 20 countries and 15 languages. It uses a visual analogue scale to assess symptom control and work productivity as well as a clinical decision support system. It is associated with an inter-operable tablet for physicians and other health care professionals. The scaling up strategy uses the recommendations of the European Innovation Partnership on Active and Healthy Ageing. The aim of the novel ARIA approach is to provide an active and healthy life to rhinitis sufferers, whatever their age, sex or socio-economic status, in order to reduce health and social inequalities incurred by the disease.
Author Correction: The Role of European Starlings (Sturnus vulgaris) in the Dissemination of Multidrug-Resistant Escherichia coli among Concentrated Animal Feeding Operations (2020)

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Analysis of linear and nonlinear photonic devices using eigenmode expension (2007)

The simulation method known as eigenmode expansion proves to be a versatile approach for modeling microphotonic devices. Originally the tool was developed for high-contrast linear designs. However, it was also extended towards nonlinear effects, such as the Kerr nonlinearity and second-harmonic generation. Here we address recent numerical and theoretical results, obtained or supported by the eigenmode method. In the linear regime, we study high quality cavities, originating from multimode interference through the multipole cancelation mechanism. For the nonlinear regime we present a photonic crystal switching device based on a symmetry breaking instability.
Thrombin generation capacity is enhanced by low antithrombin activity and depends on the activity of the related coagulation factors (2022)

The results presented in this basic research were found to be consistent with the clinical findings to date. The mechanism by which 30-50% of AT activity is set as the clinical boundary was elucidated by the thrombin generation assay.