gebhart/patrepeval huggingface dataset:
Over-production of exopolysaccharide by Lacticaseibacillus rhamnosus CNCM I-3690 strain cutbacks its beneficial effect on the host (2023)

Most lactobacilli produce extracellular polysaccharides that are considered to contribute to the probiotic effect of many strains. Lacticaseibacillus rhamnosus CNCM I-3690 is an anti-inflammatory strain able to counterbalance gut barrier dysfunction. In this study ten spontaneous variants of CNCM I-3690 with different EPS-production were generated and characterized by their ropy phenotype, the quantification of the secreted EPS and genetic analysis. Amongst them, two were further analysed in vitro and in vivo: an EPS over-producer (7292) and a low-producer derivative of 7292 (7358, with similar EPS levels than the wild type (WT) strain). Our results showed that 7292 does not have anti-inflammatory profile in vitro, and lost the capacity to adhere to the colonic epithelial cells as well as the protective effect on the permeability. Finally, 7292 lost the protective effects of the WT strain in a murine model of gut dysfunction. Notably, strain 7292 was unable to stimulate goblet cell mucus production and colonic IL-10 production, all key features for the beneficial effect of the WT strain. Furthermore, transcriptome analysis of colonic samples from 7292-treated mice showed a down-regulation of anti-inflammatory genes. Altogether, our results point out that the increase of EPS production in CNCM I-3690 impairs its protective effects and highlight the importance of the correct EPS synthesis for the beneficial effects of this strain.
AMPA receptor ligands: Synthetic and pharmacological studies of polyamines and polyamine toxins (2004)

Alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors (AMPAR), subtype of the ionotropic glutamate receptors (IGRs), mediate fast synaptic transmission in the central nervous system (CNS), and are involved in many neurological disorders, as well as being a key player in the formation of memory. Hence, ligands affecting AMPARs are highly important for the study of the structure and function of this receptor, and in this regard polyamine-based ligands, particularly polyamine toxins, are unique as they selectively block Ca2+ -permeable AMPARs. Indeed, endogenous intracellular polyamines are known to modulate the function of these receptors in vivo. In this study, recent developments in the medicinal chemistry of polyamine-based ligands are given, particularly focusing on the use of solid-phase synthesis (SPS) as a tool for the facile generation of libraries of polyamine toxin analogues. Moreover, the recent development of highly potent and very selective AMPAR ligands is described. Additionally, we provide a detailed account on the mechanism and site of action of AMPAR blockade by polyamine-based ligands, including examples of how these ligands are used as tools to study AMPAR, and a comparison with their action on other ionotropic receptors.
Intravenous administration of recombinant adenoviruses causes thrombocytopenia, anemia and erythroblastosis in rabbits (1999)

Local intravenous injection via the portal vein does not prevent systemic spread of viral vectors and the occurrence of vector-related side effects. The hematological changes observed in rabbits suggest the need for careful monitoring of hematological and rheological parameters in clinical trials.
Ab initio molecular orbital calculations of the protonation of propylene and isobutene by acidic hydroxyl groups of isomorphously substituted zeolites (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAb initio molecular orbital calculations of the protonation of propylene and isobutene by acidic hydroxyl groups of isomorphously substituted zeolitesP. Viruela-Martin, C. M. Zicovich-Wilson, and A. CormaCite this: J. Phys. Chem. 1993, 97, 51, 13713–13719Publication Date (Print):December 1, 1993Publication History Published online1 May 2002Published inissue 1 December 1993https://pubs.acs.org/doi/10.1021/j100153a047https://doi.org/10.1021/j100153a047research-articleACS PublicationsRequest reuse permissionsArticle Views253Altmetric-Citations83LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Source treatment and barriers using ORC{reg_sign} to enhance natural attenuation (1996)

ORC is a unique formulation of magnesium peroxide that releases oxygen slowly when hydrated. The compound is insoluble and releases oxygen while being converted to ordinary magnesium hydroxide which is also insoluble. ORC is packaged in exchangeable filter socks and is contacted with contaminated groundwater via an array of wells or trenches. ORC can also be made into a slurry to create permanent implants in the saturated zone, or dispersed as a free powder for the in-situ or ex-situ treatment of soil. These methods enhance the intrinsic bioremediation of aerobically degradable compounds and are summarized. ORC mediated oxygenated zones generally last four months to a year as a function of contaminant flux. The objective of the oxygen barrier is plume cut-off, however, any significant reduction of contaminant mass will reduce risk. A broad array of treatment points, in which ORC is backfilled or injected, can be effective for low-level source treatment. The points can be implemented with low-cost, small-bore technologies to achieve full remediation or risk reduction objectives. An overview of the history of the environmental applications of ORC, and the evolution of the strategies for site closure involving direct treatment of contaminants sources and control of plume migration, willmore » be the emphasis of this presentation.« less
Abstract 385: iRGD mediated delivery of neoantigens to enable immunotherapy in integrin b5-rich tumors (2022)

Abstract Pancreatic duct adenocarcinomas are known for their abundant desmoplastic stroma that acts as a barrier for drug penetration and reduces treatment efficacy. iRGD is a tumor-penetrating peptide that initially targets αv integrins expressed on tumor vasculature with its RGD motif and then is proteolytically processed to expose a CendR motif (R/KXXR/K) that interacts with neuropilin-1 (NRP-1), leading to extravasation. We investigated the mechanism of iRGD tissue penetration and found that iRGD initially targets Carcinoma Associated Fibroblasts (CAFs) and then spreads to the tumor cells in a time dependent manner. CAF targeting was dependent on integrin β5 expression and CAFs induced upregulation of integrin β5 in the adjacent tumor cells in a TGF- β dependent manner. Drugs conjugated or co-administered with iRGD can penetrate deep into tumor tissue, significantly increasing the efficacy of chemotherapeutic agents in a variety of solid tumors. Our recent data shows that KrasLSL-G12D/+ Trp53LSL-R172H/+ Pdx1-Cre (KPC) mice treated with iRGD co-administered with Gemcitabine increased survival compared to drug alone. In addition, we are using iRGD to deliver neoantigens to breast and pancreatic cancers to enable immunotherapy. These tumors have a low mutational burden and a very immunosuppressive microenvironment, rendering them resistant to such therapies. We have used iRGD, to deliver the ovalbumin 257-264 (OVAI) peptide to triple negative breast tumors, followed by adoptive T cell transfer of OT1 CD8 T cells, in order to elicit an antitumor immune response. Our preliminary data showed tumor regression in 70%, and complete response in 42% of the mice treated with iRGD plus OVA1. We are now working on adapting this strategy to pancreatic cancer. Citation Format: Tatiana Hurtado de Mendoza, Evangeline S. Mose, Gregory P. Botta, Gary B. Braun, Venkata R. Kotamraju, Randall P. French, Kodai Suzuki, Norio Miyamura, Siming Sun, Jay Patel, Tambet Teesalu, Erkki Ruoslahti, Kazuki N. Sugahara, Andrew M. Lowy. iRGD mediated delivery of neoantigens to enable immunotherapy in integrin b5-rich tumors [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 385.
Validation of a Molecular and Pathological Model for Five-Year Mortality Risk in Patients with Early Stage Lung Adenocarcinoma (2014)

This study validates the CCP score and the prognostic score as independent predictors of lung cancer death in patients with early stage lung adenocarcinoma treated with surgery alone. Patients with resected stage I lung adenocarcinoma and a high prognostic score may be candidates for adjuvant therapy to reduce cancer-related mortality.
Evaluation of Perfusion Quantification Methods with Ultrasound Contrast Agents in a Machine-Perfused Pig Liver (2016)

Machine-perfused pig liver is an excellent test bed for DCEUS quantification approaches for the study of the hepatic vascular networks. DCEUS quantification parameters (RT, MTT, and AUC) can measure relative flow changes of about 20 % and above in the liver vasculature. DCEUS quantification is a promising tool for real-time monitoring of the vascular network of tumors.
Einführung (2019)

No AccessPolymer-Aufbereitung und Kunststoff-CompoundierungOct 2019EinführungKlemens Kohlgrüber, Michael BierdelKlemens KohlgrüberSearch for more papers by this author, Michael BierdelSearch for more papers by this authorhttps://doi.org/10.3139/9783446460799.001SectionsAboutPDF ToolsAdd to FavoritesDownload CitationTrack CitationsCopy LTI LinkPDF key 'share (en)' returned an object instead of string.FacebookTwitterEmailLinkedIn previous chapternext chapter FiguresReferencesRelatedDetails 2019Pages: 1-8Print ISBN: 978-3-446-45832-1eISBN: 978-3-446-46079-9 Copyright & Permissions© 2019 Carl Hanser Verlag GmbH & Co. KGPDF downloadLoading ...
Surface-state emission enhancement in white-luminophor CdS nanocrystals using localized plasmon coupling (2008)

To make surface-state emission stronger than band-edge emission for the first time, we proposed and demonstrated plasmon coupling of the surface-state emission from the traps in these CdS nano-luminophors using localized plasmons.
Anti-BCMA/CD19 CAR T Cells with Early Immunomodulatory Maintenance for Multiple Myeloma Responding to Initial or Later-Line Therapy (2022)

CAR T cells in early lines of MM therapy could be safer and more effective than in the advanced setting, where prior studies have focused. We evaluated the safety, pharmacokinetics, and efficacy of CAR T cells in patients with low disease burden, responding to current therapy, combined with standard maintenance therapy. This article is highlighted in the In This Issue feature, p. 101.
Fabrication of half-pitch 32-45-nm SRAM patterns with EUVL (2009)

Since the k1 factor is much larger in extreme-ultraviolet lithography (EUVL) than in optical lithography, optical proximity correction (OPC) should be much simpler for patterns on EUVL masks than for those on advanced photomasks. This will facilitate the fabrication of complex device patterns with EUVL. In this study, static random-access memory (SRAM) cell patterns for the half-pitch (hp) 32- and 45-nm nodes were fabricated using two EUV exposure tools (SFET, EUV1), and their fidelity was evaluated. The levels of SRAM patterns were isolation, gate, contact, and metal. The size of the SRAM unit cell was 0.191 &mu;m<sup>2</sup> for the hp 45-nm and 0.097 &mu;m<sup>2</sup> for the hp 32-nm patterns. Most of the experiments employed SSR2, a high-resolution EUV resist. The high performance of the SFET and SSR2 enabled hp 45-nm SRAM patterns to be fabricated faithfully. However, some of the hp 32-nm patterns deviated from the mask patterns. To determine the causes of this degradation, we made a simulation analysis using the Sentaurus Lithography simulator. The main cause of the degradation was found to be resist blur. When we used MET-2D resist, which has a relatively large resist blur, the degradation became quite severe. Although the resist blur for SSR2 is about 10 nm, it is not small enough for the hp 32-nm SRAM patterns, especially for the gate and metal levels. It is necessary to reduce resist blur to improve the fidelity for this pattern size. Simulation results indicated that resist blur should be reduced to about 5 nm for hp 22-nm node device patterns.
Aerosol route to TiO2–SiO2 catalysts with tailored pore architecture and high epoxidation activity (2019)

We present the aerosol-assisted sol-gel preparation of hierarchically porous TiO2–SiO2 catalysts having a sphere-like shell morphology and a high Ti dispersion. In order to control the porosity at the micro-, meso- and macro- levels, we use the evaporation-induced self assembly (EISA) of a surfactant, possibly combined with polymer beads as hard templates. These catalysts are tested for the epoxidation of cyclohexene with cumene hydroperoxide as oxidant, and their performance is compared to the reference TS-1 zeolite. The high catalytic performance observed with the catalysts prepared by aerosol stems from their high specific surface area, but also from the short diffusion path length generated by the meso-/macro-pore architecture which provide entryways for bulky reactants and products.
34.2: Invited Paper : Development of 1m × 2m Plasma Tube Array PTA Technologies Ready for Ultra‐Large Film Display (2008)

Abstract The plasma tubes array is expected to realize the next generation of wall size display because of its wonderful characteristics such as ultra‐thin, light‐weight, flexible and low power consumption. We had successfully developed a prototype film display of 1m × 2m screen size which was consisted of 4 sub‐modules of 0.5m × 1m with the seam width under 1mm. The weight, thickness and power consumption of the film display were measured at 2.5kg, 1mm and 400W respectively.
Silicon nanocrystals on bottle resonators: Mode structure, loss mechanisms and emission dynamics (2010)

Bottle resonators can support high Q-factor whispering gallery modes (WGMs) and demonstrate a rich mode spectrum. Resonators were fabricated using a fiber tapering apparatus and were coated with a thin, smooth layer of luminescent silicon nanocrystals. The photoluminescence spectrum showed WGM peaks with Q-factors near 2,500; however, evanescent measurements showed that these modes are a composite of many modes with Q-factors exceeding 106, the highest yet seen for a silicon-nanocrystalcoated microresonators. The mode structure showed strong polarization and sensitivity to position within the bottle resonator. An analysis of loss mechanisms establishes surface roughness scattering as the limiting factor in these nanocrystal-coated bottle resonators in the absence of excited carriers.
Chronic HIV-1 Tat exposure alters anterior cingulate cortico-basal ganglia-thalamocortical synaptic circuitry, associated behavioral control, and immune regulation in male mice (2020)

HIV-1 selectively disrupts neuronal integrity within specific brain regions, reflecting differences in viral tropism and/or the regional differences in the vulnerability of distinct neuronal subpopulations within the CNS. Deficits in prefrontal cortex (PFC)-mediated executive function and the resultant loss of behavioral control are a particularly debilitating consequence of neuroHIV. To explore how HIV-1 disrupts executive function, we investigated the effects of 48 h, 2 and/or 8 weeks of HIV-1 Tat exposure on behavioral control, synaptic connectivity, and neuroimmune function in the anterior cingulate cortex (ACC) and associated cortico-basal ganglia (BG)-thalamocortical circuitry in adult, Tat transgenic male mice. HIV-1 Tat exposure increased novelty-exploration in response to novel food, flavor, and environmental stimuli, suggesting that Tat triggers increased novelty-exploration in situations of competing motivation (e.g., drive to feed or explore vs. fear of novel, brightly lit open areas). Furthermore, Tat induced adaptability in response to an environmental stressor and pre-attentive filtering deficits. The behavioral insufficiencies coincided with decreases in the inhibitory pre- and post-synaptic proteins, synaptotagmin 2 and gephyrin, respectively, in the ACC, and alterations in specific pro- and anti-inflammatory cytokines out of 23 assayed. The interaction of Tat exposure and the resultant time-dependent, selective alterations in CCL4, CXCL1, IL-12p40, and IL-17A levels in the PFC predicted significant decreases in adaptability. Tat decreased dendritic spine density and cortical VGLUT1 inputs, while increasing IL-1β, IL-6, CCL5, and CCL11 in the striatum. Alternatively, IL-1α, CCL5, and IL-13 were decreased in the mediodorsal thalamus despite the absence of synaptic changes. Thus, HIV-1 Tat appears to uniquely and systematically disrupt immune regulation and the inhibitory and excitatory synaptic balance throughout the ACC-BG-thalamocortical circuitry resulting in a loss of behavioral control.
Structural identification of mouse fecal metabolites of theaflavin 3,3′-digallate using liquid chromatography tandem mass spectrometry (2020)

Black tea consumption has been associated with many health benefits including the prevention of cancer and heart disease. Theaflavins are the major bioactive polyphenols present in black tea. Unfortunately, limited information is available on their biotransformation. In the present study, we investigated the metabolic fate of theaflavin 3,3′-digallate (TFDG), one of the most abundant and bioactive theaflavins, in mouse fecal samples using liquid chromatography/electrospray ionization tandem mass spectrometry by analyzing the MSn (n = 1–3) spectra. Four metabolites theaflavin, theaflavin 3-gallate, theaflavin 3′-gallate, and gallic acid were identified as the major mouse fecal metabolites of TFDG. Glucuronidated and sulfated, instead of methylated metabolites of theaflavin 3-gallate, theaflavin 3′-gallate, and TFDG were detected and identified as the minor mouse fecal metabolites of TFDG. Our results indicate that TFDG can be degraded in mice. Further studies on the formation of those metabolites in TFDG-treated mice in germ-free conditions are warranted. To our knowledge, this is the first report on the biotransformation of TFDG in mice.
The Impact of Electron Phonon Scattering, Finite Size and Lateral Electric Field on Transport Properties of Topological Insulators: A First Principles Quantum Transport Study (2023)

We study, using non-equilibrium Green's function simulations combined with first-principles density functional theory, the edge-state transport in two-dimensional topological insulators. We explore the impact of electron-phonon coupling on carrier transport through the protected states of two widely known topological insulators with different bulk gaps, namely stanene and bismuthene. We observe that the transport in a topological insulator with a small bulk gap (such as stanene) can be heavily affected by electron-phonon scattering, as the bulk states broaden into the bulk gap. In bismuthene with a larger bulk gap, however, a significantly higher immunity to electron-phonon scattering is observed. To mitigate the negative effects of a small bulk gap, finite-size effects are studied in stanene ribbons. The bulk gap increases in ultra-narrow stanene ribbons, but the transport results revealed no improvement in the dissipative case, as the states in the enlarged bulk gaps aren't sufficiently localized. To investigate an application, we also used topological insulator ribbons as a material for field-effect transistors with side gates imposing a lateral electric field. Our results demonstrate that the lateral electric field could offer another avenue to manipulate the edge states and even open a gap in stanene ribbons, leading to an <i>I</i><sub>ON</sub>/<i>I</i><sub>OFF</sub> of 28 in the ballistic case. These results shed light on the opportunities and challenges in the design of topological insulator field-effect transistors.
EBI2 expression is associated with B cell inflammation in human sinuses (120.35) (2012)

Abstract Epstein-Barr virus-induced G-protein coupled receptor 2 (EBI2) is important for the migration of B cells and induction of extrafollicular plasma cells within secondary lymphoid tissues. Recent studies from our group have demonstrated that many molecules associated with B cell survival and plasma cell differentiation (e.g. BAFF and immunoglobulins) were elevated in nasal polyps from patients with chronic rhinosinusitis (CRS). To investigate whether B cells or EBI2 played a role in CRS pathogenesis, expression of B cell- and EBI2-related genes in nasal tissue from normal controls and CRS patients with (CRSwNP) or without (CRSsNP) nasal polyps was assessed. EBI2 mRNA was quantitated by qRT-PCR, EBI2 protein was measured by western blot, and B cell subsets were characterized by flow cytometry. Expression of EBI2 mRNA was significantly elevated in polyp tissue (p&amp;lt;0.001) and correlated with mRNA expression of CD20 (r=0.71; p&amp;lt;0.0001) and CH25H, an enzyme responsible for production of an EBI2 ligand (r=0.65; p&amp;lt;0.001). EBI2 protein was also elevated in polyps (p&amp;lt;0.01, n=10). In addition, B cells (CD20+) and plasma cells (CD138+) were significantly elevated in polyps compared to nasal tissue from normal controls (p&amp;lt;0.05). Collectively, these data suggest that elevated EBI2 may facilitate B cell migration and differentiation in the nasal mucosa, especially nasal polyps. These findings provide new insights into the pathogenesis of CRS and may indicate potential new therapeutic targets.
Importance of Early Follow-up Colonoscopy in Patients at High Risk for Colorectal Polyps (2013)

The polyp miss rate in patients at high risk for colorectal polyps was higher than expected. Efforts are needed to reduce miss rates and improve the quality of colonoscopy. Also, early follow-up colonoscopy is mandatory, especially in patients at high risk.
Rhodium-catalysed asymmetric hydrogenation as a valuable synthetic tool for the preparation of chiral drugs (2012)

During the last few decades, rhodium-catalysed asymmetric hydrogenation of diverse alkene classes has emerged as a powerful synthetic tool in the pharmaceutical industry, contributing to the manufacturing of chiral drugs, recent drug candidates for clinical trials, and major synthetic precursors of drugs. Numerous efficient chiral rhodium complexes, most of which are derived from enantiopure phosphorus ligands, have been employed for the preparation of chiral drugs and intermediates thereof. This review article is intended to provide an updated overview of the most striking contributions in this field, organised according to substrate class: acrylate derivatives, itaconate derivatives, α-substituted enamides, α-arylenol acetates, and minimally functionalised olefins.