gebhart/patrepeval huggingface dataset:
Regulated expression of P210 Bcr-Abl during embryonic stem cell differentiation stimulates multipotential progenitor expansion and myeloid cell fate (2000)

P210 Bcr-Abl is an activated tyrosine kinase oncogene encoded by the Philadelphia chromosome associated with human chronic myelogenous leukemia (CML). The disease represents a clonal disorder arising in the pluripotent hematopoietic stem cell. During the chronic phase, patients present with a dramatic expansion of myeloid cells and a mild anemia. Retroviral gene transfer and transgenic expression in rodents have demonstrated the ability of Bcr-Abl to induce various types of leukemia. However, study of human CML or rodent models has not determined the direct and immediate effects of Bcr-Abl on hematopoietic cells from those requiring secondary genetic or epigenetic changes selected during the pathogenic process. We utilized tetracycline-regulated expression of Bcr-Abl from a promoter engineered for robust expression in primitive stem cells through multilineage blood cell development in combination with the in vitro differentiation of embryonal stem cells into hematopoietic elements. Our results demonstrate that Bcr-Abl expression alone is sufficient to increase the number of multipotent and myeloid lineage committed progenitors in a dose-dependent manner while suppressing the development of committed erythroid progenitors. These effects are reversible upon extinguishing Bcr-Abl expression. These findings are consistent with Bcr-Abl being the sole genetic change needed for the establishment of the chronic phase of CML and provide a powerful system for the analysis of any genetic change that alters cell growth and lineage choices of the hematopoietic stem cell.
How Do Socket Size Adjustments during Ambulation Affect Residual Limb Fluid Volume? Case Study Results (2018)

ABSTRACT Introduction Adjustable prosthetic sockets may be useful in management of limb volume in individuals with amputation. Incrementally increasing socket size during ambulation may increase residual limb fluid volume, deterring daily volume loss without sacrificing gait stability. Materials and Methods Participants with transtibial limb loss wore motor-controlled cabled-panel adjustable sockets while residual limb fluid volume was monitored using bioimpedance analysis. Participants walked on a treadmill with a safety harness while socket size was adjusted to establish users' ranges of acceptable sizes and optimal size. Then single controlled size adjustments were made. In longer duration testing outside the laboratory, participants used a portable system controlled by a mobile phone app to incrementally increase or decrease their socket size. Results For the two participants tested, comfortable socket sizes spanned as low as a 1.6% range of socket volume to as high as a 6.1% range. Limb fluid volume changed in proportion to socket size when adjustments were near users' optimal socket size setting. Conclusions Adjustable sockets are a viable means to manipulate limb fluid volume during ambulation. Incremental changes in socket size, approximately 0.33% socket volume, were sufficient to induce detectable changes in socket fit.
263 The flammability limit of the high temperature air combustion (2010)

The flammability jimit ef the
ANGI-03. PHARMACOLOGICAL TARGETING OF APELIN/APLNR SIGNALING BLUNTS THERAPY RESISTANCE TO VEGFA/VEGFR2 ANTI-ANGIOGENIC TREATMENT IN GLIOBLASTOMA (2019)

Abstract Anti-angiogenic therapy of glioblastoma with bevacizumab, a vascular endothelial growth factor-A (VEGFA) blocking antibody, may accelerate tumor cell invasion and induce alternative angiogenic pathways. We investigated the roles of the pro-angiogenic receptor APLNR and its cognate ligand apelin in VEGFA/VEGFR2 anti-angiogenic therapy against distinct subtypes of glioblastoma. In proneural glioblastoma, apelin levels were downregulated by VEGFA or VEGFR2 blockade by use of bevacizumab or ramucirumab, respectively. A central role for apelin/APLNR in controlling glioblastoma vascularization was corroborated in a serial implantation model of the angiogenic switch that occurs in human glioblastoma. Apelin and APLNR are broadly expressed in human glioblastoma, and knockdown or knockout of APLN in orthotopic models of proneural or classical glioblastoma subtypes massively reduced glioblastoma vascularization as compared with controls. What is more, direct infusion of the bioactive peptide apelin-13 was able to rescue this vascular loss-of-function phenotype, demonstrating the specific control of tumor vascularization by apelin/APLNR signaling. While high levels of apelin correlated with reduced tumor cell invasiveness, the reduction in apelin expression led to accelerated glioblastoma cell invasion. Analysis of stereotactic glioblastoma biopsies from patients as well as from in vitro and in vivo experiments revealed increased dissemination of APLNR-positive tumor cells when apelin levels were reduced. Most interestingly, application of apelin-F13A, a mutant APLNR ligand, blocked both tumor angiogenesis and glioblastoma cell invasion. Furthermore, co-targeting VEGFR2 and APLNR synergistically improved survival of mice bearing proneural glioblastoma. In summary, we show that apelin/APLNR signaling controls glioblastoma angiogenesis and invasion directly, and that both pathological features are blunted by apelin-F13A. We suggest that apelin-F13A can improve the efficiency and reduce the side effects of established anti-angiogenic treatments for distinct glioblastoma subtypes.
HIV-1 DNA Testing in Viremic Patients Identifies More Drug Resistance Than HIV-1 RNA Testing (2023)

HIV-1 DNA testing identified more resistance than HIV-1 RNA testing in most patients with viremia and may be informative in patients whose plasma virus reverts to wild-type following therapy discontinuation.
Fibroblast Growth Factor-23 (FGF-23) e Sindrome Cardiorenale (2015)

Abstract non disponibile (Cardionephrology)
Resonant laser diagnostics of a planar plasma opening switch (1995)

The plasma opening switch (POS) is an integral part of inductive store pulsed power systems. Using flashboards coated with BaO and a dye laser tuned to the 493.4 nm ground state transition of the singly-ionized barium ion, resonant laser diagnostics have been employed to image the switch plasma and provide a measurement of the plasma density during conduction and opening. Gaps open during the conduction phase with their position in the inter-electrode region depending upon the initial fill plasma. There is little axial motion of the plasma, contrary to the predictions of analytical hydro model calculations performed using the measured switch parameters. This discrepancy may be due to a finite lifetime for ions in the switch that is less than the conduction time resulting in a larger effective mass. From a functional point of view modified bipolar model calculations best fit the data.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Targeting Cancer Cells and Tumor Microenvironment in Preclinical and Clinical Models of Hodgkin Lymphoma Using the Dual PI3Kδ/γ Inhibitor RP6530 (2018)

Our results support PI3Kδ/γ inhibition as a novel therapeutic strategy that targets both malignant cells and the TME to treat patients with Hodgkin lymphoma.
[Specific role of sex steroids on the pituitary level. Sex steroids and anterior pituitary secretion]. (1978)

Although sex steroids were known to play a role in the control of LH, FSH, TSH and prolactin secretion, in vivo experiments could not discriminate between hypothalamic and pituitary sites of action. In this study, the specific action of sex steroids at the anterior pituitary level could be achieved using rat adenohypophyseal cells in primary culture. While estrogens stimulated the sensitivity of the LH and FSH responses to LHRH, androgens had differential effects on the secretion of the two gonadotropins: marked inhibition of LH and stimulation of FSH secretion. Progesterone, on the other hand, while having no effect in the absence of estrogens, could reverse the stimulatory effect of estrogens on LH release while it led to a stimulation of FSH secretion. Estrogens and thyroid hormone exert respective stimulatory and inhibitory effects on TSH secretion by a direct action at the pituitary level. These effects appear to be mediated changes of the level of adenohy-pophyseal TRH receptors. A close correlation was observed between the specificity of binding of the dopamine agonist (3H)dihydroergocryptine and the control of prolactin release in cells in culture, thus supporting the physiological importance of the dopamine receptor in the control of prolactin release. The high degree precision of this system permits assessment of activity of not only dopamine agonists and antagonists, but also of compounds having mixed agonist-antagonistic activity. Preincubation of anterior pituitary cells with 17beta-estradiol not only stimulated basal and TRH-induced prolactin release but, more unexpectedly, led to an almost complete reversal of the inhibitory effect of dopamine agonists on prolactin secretion. Besides its own interest, the adenohypophyseal cell culture system could well be used as a model system for study of the interaction between estrogens and dopaminergic action.
F0 contour prediction with a deep belief network-Gaussian process hybrid model (2013)

In this work we look at using non-parametric, exemplar-based regression for the prediction of prosodic contour targets from textual features in a speech synthesis system. We investigate the performance of Gaussian Process regression on this task when the covariance kernel operates on a variety of input feature spaces. In particular, we consider non-linear features extracted via Deep Belief Networks. We motivate the use of this hybrid model by considering the initial deep-layer model as a feature extractor that can summarize high-level structure from the raw inputs to improve the regression of an exemplar-based model in the second part of the approach. By looking at both objective metrics and perceptual listening tests, we evaluate these proposals against each other, and against the standard clustering-tree techniques implemented in parametric synthesis for the prediction of prosodic targets.
Telomere-Maintenance Mechanisms in Soft-Tissue Malignant Fibrous Histiocytomas (2009)

More detailed analysis will be needed to identify the most valuable prognostic factor in patients with malignant fibrous histiocytoma, and a more thorough understanding of telomere biology may give an indication of telomere-targeting therapy in the future.
Adhesive Cements That Bond Soft Tissue Ex Vivo (2019)

The aim of the present study was to evaluate the soft tissue bond strength of a newly developed, monomeric, biomimetic, tissue adhesive called phosphoserine modified cement (PMC). Two types of PMCs were evaluated using lap shear strength (LSS) testing, on porcine skin: a calcium metasilicate (CS1), and alpha tricalcium phosphate (αTCP) PMC. CS1 PCM bonded strongly to skin, reaching a peak LSS of 84, 132, and 154 KPa after curing for 0.5, 1.5, and 4 h, respectively. Cyanoacrylate and fibrin glues reached an LSS of 207 kPa and 33 kPa, respectively. αTCP PMCs reached a final LSS of ≈110 kPa. In soft tissues, stronger bond strengths were obtained with αTCP PMCs containing large amounts of amino acid (70-90 mol%), in contrast to prior studies in calcified tissues (30-50 mol%). When αTCP particle size was reduced by wet milling, and for CS1 PMCs, the strongest bonding was obtained with mole ratios of 30-50% phosphoserine. While PM-CPCs behave like stiff ceramics after setting, they bond to soft tissues, and warrant further investigation as tissue adhesives, particularly at the interface between hard and soft tissues.
Ultra-low loss Si_3N_4 waveguides with low nonlinearity and high power handling capability (2010)

We investigate the nonlinearity of ultra-low loss Si3N4-core and SiO2-cladding rectangular waveguides. The nonlinearity is modeled using Maxwell's wave equation with a small amount of refractive index perturbation. Effective n2 is used to describe the third-order nonlinearity, which is linearly proportional to the optical intensity. The effective n2 measured using continuous-wave self-phase modulation shows agreement with the theoretical calculation. The waveguide with 2.8-μm wide and 80-nm thick Si3N4 core has low loss and high power handling capability, with an effective n2 of about 9×10(-16) cm2/W.
Preclinical anti-tumour activity of HexaBody-CD38, a next-generation CD38 antibody with superior complement-dependent cytotoxic activity (2023)

Genmab.
Observation of Wγγ triboson production in proton-proton collisions at s=13 TeV with the ATLAS detector (2023)

This letter reports the observation of W(ℓν)γγ production in proton-proton collisions. This measurement uses the full Run 2 sample of events recorded at a center-of-mass energy of s=13 TeV by the ATLAS detector at the LHC, corresponding to an integrated luminosity of 140 fb−1. Events with a leptonically-decaying W boson and at least two photons are considered. The background-only hypothesis is rejected with an observed and expected significance of 5.6 standard deviations. The inclusive fiducial production cross section of W(eν)γγ and W(μν)γγ events is measured to be σfid=13.8±1.1(stat)+2.1−2.0(syst)±0.1(lumi) fb, in agreement with the Standard Model prediction.
Angiogenesis: role of calcium-mediated signal transduction. (1995)

During angiogenesis, endothelial cells react to stimulation with finely tuned signaling responses. The role of calcium-regulated signaling in angiogenesis has not been defined. This study investigated the calcium dependency of endothelial cell proliferation and invasion by using an inhibitor of ligand-stimulated calcium influx, CAI (carboxy-amidotriazole). Incubation with CAI significantly inhibited proliferation of human umbilical vein endothelial cells (HUVECs) in response to serum (IC50 = 1 microM) or basic fibroblast growth factor (FGF2; P2 < 0.005 at 10 microM). Statistically significant inhibition of HUVEC adhesion and motility to basement membrane proteins laminin, fibronectin, and type IV collagen was demonstrated (adhesion, P2 < 0.004-0.01; motility, P2 < 0.009-0.018). Marked inhibition of native and FGF2-induced gelatinase activity was shown by zymogram analysis and was confirmed by Northern blot analysis. CAI inhibited HUVEC tube formation on Matrigel and inhibited in vivo angiogenesis in the chicken chorioallantoic membrane assay, 67% at 20 microM and 56% at 10 microM compared with 16% for an inactive CAI analog or 9% for 0.1% dimethyl sulfoxide control. Incubation of HUVECs with CAI and/or FGF2 followed by immunoprecipitation with anti-phosphotyrosine antibody showed inhibition of FGF2-induced tyrosine phosphorylation of proteins in the range 110-150 kDa. These results suggest that calcium-regulated events are important in native and FGF2-stimulated HUVEC proliferation and invasion, perhaps through regulation of FGF2-induced phosphorylation events, and indicate a role for calcium in the regulation of angiogenesis in vivo.
A torsional artificial muscle from twisted nitinol microwire (2017)

Nitinol microwires of 25 &mu;m in diameter can have tensile actuation of up to 4.5% in less than 100 ms. A work density of up to 480 MPa can be achieved from these microwires. In the present work, we are showing that by twisting the microwires in form of closed-loop two-ply yarn we can create a torsional actuator. We achieved a revisable torsional stroke of 46&deg;/mm with peak rotational speed of up to 10,000 rpm. We measured a gravimetric torque of up to 28.5 N&bull;m/kg which is higher than the 3 – 6 N&bull;m/kg for direct-drive commercial electric motors. These remarkable performance results are comparable to those of guest-infiltrated carbon nanotube twisted yarns.
Aggregating data center measurements for availability analysis (2020)

Summary A data center infrastructure is composed of heterogeneous resources divided into three main subsystems: IT (processor, memory, disk, network, etc.), power (generators, power transformers, uninterruptible power supplies, distribution units, among others), and cooling (water chillers, pipes, and cooling tower). This heterogeneity brings challenges for collecting and gathering data from several devices in the infrastructure. In addition, extracting relevant information is another challenge for data center managers. While seeking to improve the cloud availability, monitoring the entire infrastructure using a variety of (open source and/or commercial) advanced monitoring tools, such as Zabbix, Nagios, Prometheus, CloudWatch, AzureWatch, and others is required. It is often common to use many monitoring systems to collect real‐time data for data center components from different subsystems. Such an environment brings an inherent challenge stemming from the need to aggregate and organize the whole collected infrastructure data and measurements. This first step is necessary prior to obtaining any valuable insights for decision‐making. In this paper, we present the Data Center Availability (DCA) System, a software system that is able to aggregate and analyze data center measurements aimed toward the study of DCA. We also discuss the DCA implementation and illustrate its operation, monitoring a small University research laboratory data center. The DCA System is able to monitor different types of devices using the Zabbix tool, such as servers, switches, and power devices. The DCA System is able to automatically identify the failure time seasonality and trend present in the collected data from different devices of the data center.
Chemical Complementation Assays (2008)

Abstract Originally published in: Enzyme Assays. Edited by Jean‐Louis Reymond. Copyright © 2006 Wiley‐VCH Verlag GmbH &amp; Co. KGaA Weinheim. Print ISBN: 3‐527‐31095‐1 The sections in this article are Introduction Complementation Assays Introduction Early Complementation Assays Enzymology by Complementation Directed Evolution by Complementation Development of Chemical Complementation Introduction Three‐hybrid Assay Original Yeast Three‐hybrid System Dexamethasone–Methotrexate Yeast Three‐hybrid System Technical Considerations Other Three‐hybrid Systems Chemical Complementation Selection Scheme and Model Reaction Results General Considerations Related Methods Applications of Chemical Complementation Introduction Enzyme‐Inhibitor Interactions Rationale Screen Strategy Enzyme Library Screen General Considerations Glycosynthase Evolution Rationale Selection Scheme Glycosynthase Assay Directed Evolution General Considerations Conclusion
MYC promotes tryptophan uptake and metabolism by the kynurenine pathway in colon cancer (2019)

Tumors display increased uptake and processing of nutrients to fulfill the demands of rapidly proliferating cancer cells. Seminal studies have shown that the proto-oncogene MYC promotes metabolic reprogramming by altering glutamine uptake and metabolism in cancer cells. How MYC regulates the metabolism of other amino acids in cancer is not fully understood. Using high-performance liquid chromatography (HPLC)-tandem mass spectrometry (LC-MS/MS), we found that MYC increased intracellular levels of tryptophan and tryptophan metabolites in the kynurenine pathway. MYC induced the expression of the tryptophan transporters SLC7A5 and SLC1A5 and the enzyme arylformamidase (AFMID), involved in the conversion of tryptophan into kynurenine. SLC7A5, SLC1A5, and AFMID were elevated in colon cancer cells and tissues, and kynurenine was significantly greater in tumor samples than in the respective adjacent normal tissue from patients with colon cancer. Compared with normal human colonic epithelial cells, colon cancer cells were more sensitive to the depletion of tryptophan. Blocking enzymes in the kynurenine pathway caused preferential death of established colon cancer cells and transformed colonic organoids. We found that only kynurenine and no other tryptophan metabolite promotes the nuclear translocation of the transcription factor aryl hydrocarbon receptor (AHR). Blocking the interaction between AHR and kynurenine with CH223191 reduced the proliferation of colon cancer cells. Therefore, we propose that limiting cellular kynurenine or its downstream targets could present a new strategy to reduce the proliferation of MYC-dependent cancer cells.
Integration challenges of Cu pillars with extreme wafer thinning for 3D stacking and packaging (2011)

In this paper, we report on the development of Cu pillars and their impact on the subsequent thinning process for 3D applications. As the Cu pillars have a height of tens of microns (typically between 50–100µm), controlling the total thickness variation (TTV) after wafer thinning is becoming even more challenging. The Cu pillars are processed after completion of the Back End of Line (BEOL) with a target thickness of 50µm for a diameter of 80µm and a pitch of 200µm. The key challenge for 3D integration is the control of the wafer TTV after back grinding in order to allow TSV reveal. After optimization of the temporary wafer bonding in presence of high topography induced by 50µm high Cu pillars, a TTV after thinning below 5µm is achieved, which is comparable to the TTV obtained after wafer thinning without topography.
Motif kernel generated by genetic programming improves remote homology and fold detection (2007)

The GPkernel gives particularly good results on the more difficult fold recognition problem compared to the other methods. This is mainly because the method creates motif sets that describe similarities among subgroups of both the related and unrelated proteins. This rich set of motifs give a better description of the similarities and differences between different folds than do previous motif-based methods.
Two New Protease‐Inhibiting Glycosphingolipids from Buddleja crispa. (2007)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Three Types of Behaviour of Multiwall Carbon Nanotubes in Reactions with Intercalating Agents (2000)

Abstract Interaction between multiwall nanotubes and intercalating agents (K or FeCl3) was studied using seven different nanotube materials. SEM, TEM, X-ray diffraction, Raman spectroscopy and other techniques were employed to study the reaction products. It was found that the reaction way is determined by nanotube structural type and that nanotubes may exhibit three types of reaction behaviour: intershell intercalation: no-reaction; and intercalation-assisted break-up.