gebhart/patrepeval huggingface dataset:
Stability Analysis in Face Milling Operations: Part 1 — Theory of Stability Lobe Prediction (1997)

Abstract This paper involves development of an algorithm to predict stability lobes in face milling operation. The modeling is based on frequency domain analysis and is comprehensive. It considers the effects of insert and cutter geometry, cutting conditions, process non-linearity, entry and exit angles and the fully dimensional structural dynamics of both the machine tool and workpiece, including axial dynamics which are often neglected by many researchers. In addition, the predictive capability of stability lobes by the presented models is compared with those results obtained by time domain simulations.
Composite, Grid-Stiffened Panel Design for Post Buckling Using Hypersizer (Trademark) (2001)

Due to weight and cost goals. a grid-stiffened panel concept is being used for redesign of a structural component on the Minotaur OSP space launch vehicle. By designing the structural panels to carry operational loads past the point of initial buckling (local post- buckling). the resulting grid stiffened panel concept is lighter and 300- less costly to manufacture than other design candidates such as the existing honeycomb sandwich panel concept flown today. During June 2001 in Seattle. Boeing performed a structural certification experiment of a composite, grid stiffened, cylindrical panel loaded in axial compression. Pretest predictions were made for linear elastic (bifurcation) buckling, and non-linear post buckling. The tools used for pretest analysis were HyperSizer(Registered). and the FEM based tools MSC/NASTRAN(TradeMade) and STAGS(TradeMade). Local buckling of the facesheet triangular shaped skin pocket occurred at a load of around 230 (lb/in). The test panel was able to sustain considerable additional loading. with post buckling failure occurring at 1320 (lb/in). The HyperSizer post buckling pretest prediction was 1300 (lb/in), the STAGS pretest prediction was 1250 (lb/in), and the MSC/NASTRAN pretest prediction ranged from 1425 to 2000 (lb/in). HyperSizer's implementation of local post buckling based on an effective width approach is presented.
Development of SiGe Indentation Process Control to Enable Stacked Nanosheet FET Technology (2020)

The methodology of measuring the lateral etch, or indentation, of SiGe nanosheets by using optical scatterometry, x-ray fluorescence, and machine learning algorithms is presented and discussed. Stacked nanosheet device structures were fabricated with different etch conditions in order to induce variations in the indent. It was found that both scatterometry in conjunction with Spectral Interferometry and novel interpretation algorithms as well as TEM calibrated LE-XRF are suitable techniques to quantify the indent. Machine learning algorithms enabled an additional solution path by combining LE-XRF data with scatterometry spectra therefore avoiding the need for a full optical model.
TISSUE ENGINEERED STENTS CREATED FROM CHONDROCYTES (2001)

This study demonstrates the feasibility of creating cartilaginous stents in vitro and in vivo using chondrocyte seeded polymer matrices. This technology may be useful clinically for stricture disease in the genitourinary tract.
Abstract 143: A platform for deep evolutionary profiling of cancer resistance (2023)

Abstract Despite the introduction of a number of new therapeutic classes including targeted inhibitors, immunotherapy, and antibody-drug conjugates, the ability to achieve long-term disease control in the majority of patients with advanced cancer remains elusive due to the emergence of resistance. While our understanding of resistance mechanisms continues to expand through preclinical and clinical sequencing efforts, comprehensive approaches to map the distribution of potential resistance mechanisms in a given cell line and drug context require the development of novel approaches. Here, we describe the design of a platform for profiling cancer resistance at scale. Beginning with a single sensitive parental cancer cell line, the EGFR-mutant lung cancer line PC9, we optimize pre-existing diversity using a combination of single-cell subcloning and alkylator mutagenesis, and generate 50 independently evolved clones resistant to the EGFR inhibitor osimertinib. Profiling of this library via multiplexed whole genome sequencing (WGS) demonstrates convergent mutations in the MAPK pathway. Taking a similar approach, we profile the NTRK1-fusion-driven KM12 colon cancer cell line, yielding convergent, clinically observed, on-target resistance mutations to the NTRK inhibitor larotrectinib. Taken together, these results provide an initial proof-of-concept for the high-throughput generation of viable drug-resistant cancer clones. Future efforts to increase mutational diversity as well as assess additional drug and disease contexts will broaden the utility of this approach. Citation Format: Arvind Ravi, Sainikhil Sontha, Maisha Chowdhury, Jacob Smigiel, Matthew Rees, Brian Danysh, Jennifer Roth, Laxmi Parida, Eric Lander, Gad Getz. A platform for deep evolutionary profiling of cancer resistance [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 143.
Lysine-Derived Charge-Altering Releasable Transporters: Targeted Delivery of mRNA and siRNA to the Lungs (2023)

Targeted delivery of nucleic acid therapeutics to the lungs could transform treatment options for pulmonary disease. We have previously developed oligomeric charge-altering releasable transporters (CARTs) for in vivo mRNA transfection and demonstrated their efficacy for use in mRNA-based cancer vaccination and local immunomodulatory therapies against murine tumors. While our previously reported glycine-based CART-mRNA complexes (<b>G-CARTs/</b>mRNA) show selective protein expression in the spleen (mouse, >99%), here, we report a new lysine-derived CART-mRNA complex <b>(K-CART/</b>mRNA<b>)</b> that, without additives or targeting ligands, shows selective protein expression in the lungs (mouse, >90%) following systemic IV administration. We further show that by delivering siRNA using the <b>K-CART</b>, we can significantly decrease expression of a lung-localized reporter protein. Blood chemistry and organ pathology studies demonstrate that <b>K-CARTs</b> are safe and well-tolerated. We report on the new step economical, organocatalytic synthesis (two steps) of functionalized polyesters and oligo-carbonate-co-α-aminoester <b>K-CARTs</b> from simple amino acid and lipid-based monomers. The ability to direct protein expression selectively in the spleen or lungs by simple, modular changes to the CART structure opens fundamentally new opportunities in research and gene therapy.
Harnessing natural killer cells for the treatment of multiple myeloma (2022)

Multiple myeloma (MM) is hematologic malignancy that is associated with profound immune alterations. Myeloma cells are susceptible to killing by natural killer (NK) cells but acquire the ability to elude NK cell surveillance by avoiding recognition and suppressing NK cell function. Major advances in the treatment of multiple myeloma have been achieved by effective new drugs that redirect NK cells and enhance their function. Despite significant progress, myeloma remains incurable and novel treatment approaches are required. Strategies to take advantage of the intrinsic antitumor properties of NK cells to treat MM represent a novel immunotherapeutic approach. One such strategy is adoptive NK cell therapy that consist of infusions of NK cells that have been propagated ex vivo. Adoptive NK cell therapy encompasses contemporary genetic engineering strategies such as chimeric antigen receptor (CAR)-engineered NK cells. An alternative approach involves the use of pharmacologic agents to enhance NK cell activity against myeloma. NK cell-modulating therapies can be used to bolster the function of endogenous NK cells or to enhance the efficacy of adoptively infused NK cells. Here, we review the mechanistic complexities influencing NK cell activity in MM and highlight a variety of innovative NK cell-based strategies being developed for the treatment of MM.
Paracrine stimulation of surfactant secretion by extracellular ATP in response to mechanical deformation (2005)

We developed a heterologous system to study the effect of mechanical deformation on alveolar epithelial cells. First, isolated primary rat alveolar type II (ATII) cells were plated onto silastic substrata coated with fibronectin and maintained in culture under conditions where they become alveolar type I-like (ATI) cells. This was followed by a second set of ATII cells labeled with the nontransferable, vital fluorescent stain 5-chloromethylfluorescein diacetate to distinguish them from ATI cells. By morphometric analysis, equibiaxial deformation (stretch) of the silastic substratum induced comparable changes in cell surface area for both ATII and ATI cells. Surfactant lipid secretion was measured using cells metabolically labeled with [(3)H]choline. In response to 21% tonic stretch for 15 min, ATII cells seeded with ATI cells secreted nearly threefold more surfactant lipid compared with ATII cells seeded alone. ATI cells did not secrete lipid in response to stretch. The enhanced lipid secretion by ATII plus ATI cocultures was inhibited by treatment with apyrase and adenosine deaminase, suggesting that ATP release by ATI cells enhanced surfactant lipid secretion at 21% stretch. This was confirmed using a luciferase assay where, in response to 21% stretch, ATI cells released fourfold more ATP than ATII cells. Because ATI cells release significantly more ATP at a lower level of stretch than ATII cells, this supports the hypothesis that ATI cells are mechanosensors in the lung and that paracrine stimulation of ATII cells by extracellular ATP released from ATI cells plays a role in regulating surfactant secretion.
Fiber-Optic Sensors Based on Degradable Polymers (1994)

A major deficiency in the use of sensors based on irreversible chemistries is that they cannot be regenerated and are therefore limited to a single use. Our laboratory has developed methods to use slow release polymers as a way to renew the supply of reagent(s) available at the fiber tip. Previous work has demonstrated this approach with two examples: a pH sensor based on a pH sensitive dye entrapped in ethylene vinyl acetate copolymer (EVA) and a sensor based on an immunoassay employing an antibody and an antigen entrapped in EVA matrices. We investigated the preparation of fiber-optic sensors using reagents confined in degradable microspheres that release over extended time periods. This paper describes the use of poly(lactide-glycolide) copolymers for the preparation of reagent containing microspheres. The release rates, sensor configurations and response times of various sensors prepared with these microspheres are described.
Nuclear magnetic resonance studies of intramolecular motions and side-chain interactions in water-soluble polyamino acids (1970)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNuclear magnetic resonance studies of intramolecular motions and side-chain interactions in water-soluble polyamino acidsF. J. Joubert, N. Lotan, and H. A. ScheragaCite this: Biochemistry 1970, 9, 10, 2197–2211Publication Date (Print):May 1, 1970Publication History Published online1 May 2002Published inissue 1 May 1970https://pubs.acs.org/doi/10.1021/bi00812a025https://doi.org/10.1021/bi00812a025research-articleACS PublicationsRequest reuse permissionsArticle Views56Altmetric-Citations41LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Biomechanical analysis of pulmonary contusion in motor vehicle crash victims: a crash injury research and engineering network (ciren) study - biomed 2009. (2009)

Pulmonary contusion is the most common thoracic soft tissue injury encountered in motor vehicle crashes and is seen in 10-17% of all trauma admissions. This study presents a biomechanical and radiological analysis with the goal of quantifying pulmonary contusion resulting from motor vehicle crashes in order to illustrate the relationships between crash characteristics, contusion severity, and patient outcome. The 20 patients selected for this study were involved in motor vehicle crashes and subsequently enrolled in the Crash Injury and Research Engineering Network (CIREN) program at Wake Forest University Baptist Medical Center. Demographic data, sustained injuries, and crash characteristics were obtained through medical records and the CIREN database for all patients in the study. For each patient, the first chest computed tomography (CT) scan following the crash was segmented using a semi-automated approach to obtain volumes of trapped air, total lung, healthy lung, and high attenuation lung representing contused tissue. Three-dimensional models of the healthy and contused lung tissue were created for each patient. Rib fractures were present in 75% of patients and a substantial proportion of patients with pulmonary contusion injuries were involved in near side collisions. The near side door was identified as the most commonly involved component in pulmonary contusion injuries. The methodology and analysis presented in this study between crash characteristics, pulmonary contusion severity, and patient outcome are data that may contribute to future improvements in motor vehicle safety.
ChemInform Abstract: Silanediols: A New Class of Potent Protease Inhibitors. (1998)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Uptake of antioxidants in surfactant liposomes by cultured alveolar type II cells is enhanced by SP-A (1993)

Antioxidant delivery may be targeted toward the alveolar epithelium by encapsulating superoxide dismutase (SOD) and catalase in liposomes made from pulmonary surfactant. We studied whether antioxidant-surfactant liposomes increase cellular antioxidant activity in alveolar type II cells and whether this effect is influenced by the presence of surfactant protein A (SP-A). Cu,Zn SOD and catalase were encapsulated in liposomes made from synthetic phospholipids with or without 5% SP-A or from natural cow surfactant. Alveolar type II cells from adult rats were preincubated for 20 h, and liposome mixtures were added for 24 h, followed by measurement of cellular SOD and catalase activities (U/mg DNA). Antioxidant-surfactant liposomes increased alveolar type II cell antioxidant activity sharply. Uptake of SOD/catalase from liposomes with synthetic phospholipids and SP-A was twice that from liposomes without SP-A and did not further improve in the presence of SP-B and -C. Encapsulation of antioxidants diminished the surface activity of the surfactant liposomes, but this feature was absent in the presence of SP-A. These data suggest that: 1) antioxidant-surfactant liposomes augment alveolar type II cell antioxidant activity, 2) liposomal uptake is facilitated by the presence of SP-A, and 3) inhibition of surface activity of surfactant by encapsulated antioxidants can be reversed by SP-A.
Planar Photonic Crystal Biosensor for Quantitative Label‐Free Cell Attachment Microscopy (2015)

In this study, a planar-surface photonic crystal (PC) biosensor for quantitative, kinetic, label-free imaging of cell-surface interactions is demonstrated. The planar biosensor surface eliminates external stimuli to the cells caused by substrate topography to more accurately reflect smooth surface environment encountered by many cell types in vitro. Here, a fabrication approach that combines nanoreplica molding and a horizontal dipping process is used to planarize the surface of the PC biosensor. The planar PC biosensor maintains a high detection sensitivity that enables the monitoring of live cell-substrate interactions with spatial resolution sufficient for observing intracellular attachment strength gradients and the extensions of filopodia from the cell body. The evolution of cell morphology during the attachment and spreading process of 3T3 fibroblast cells is compared between planar and grating-structured PC biosensors. The planar surface effectively eliminates the directionally biased cellular attachment behaviors that are observed on the grating-structured surface. This work represents an important step forward in the development of label-free techniques for observing cellular processes without unintended external environmental modulation.
Complex halides of transition metals. Part VII. The vibrational spectra of the complex anions TiX62–, ZrX62–, and HfX62–, where X = Cl or Br (1969)

The solid-state far-infrared and laser Raman spectra of the salts [Et2NH2]2MX6, where M = Ti, Zr, or Hf, and X = Cl or Br, have been recorded. Force-constant calculations were carried out by employing a modified simple valence force field (MSVFF). For several of these salts the infrared-active modes ν3 and ν4 were found to be split.
Reply to Schubert et al.: Regarding critique of highly multiplexed technologies (2014)

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans the biological, physical, and social sciences.
Design and fabrication of freeform reflector for automotive lighting system (2008)

2008-2009 > Academic research: refereed > Publication in refereed journal
Identification of Vibration Transfer Component by Combined Use of Transfer Path Analysis and Mutual Mean Compliance (2020)

In the development of mechanical products, prediction of NHV (Noise, Vibration and Harshness) using CAE (Computer Aided Engineering) is carried out. As one of the vibration generation factors on car body, excitation from road surface to tire propagates through the frame and causes unpleasant vibration on driver’s room. In this research, first, we specify the vibration transmission path from the excitation source to the evaluation point using TPA. Next, we use MMC to calculate the sensitivity to the response reduction of each element in the target path. Finally, we apply an element extraction algorithm with a bandwidth to this sensitivity to extract elements that can reduce the response of the target frequency band.
In vitro and in vivo hemocompatibility evaluation of a new dermatan sulfate-modified PET patch for vascular repair surgery (2017)

International audience
Online Control of Stick-Slip in Rotary Steerable Drilling (2017)

Rotary Steerable System is the current state of art of directional steering systems which has been developed for well drilling to achieve high reservoir productivity and to improve accessibility of oil reservoirs in complex locations. This work presents an online control scheme for real time optimization of drilling parameters to maximize rate of penetration and minimize the deviation from the planned well bore trajectory, and stick-slip oscillations. Nonlinear model for the drilling operation was developed using energy balance equation, where rock specific energy is used to calculate the minimum power required for a given rate of penetration. A proposed mass spring system was used to represent the phenomena of stick-slip oscillation. Detailed mathematical formulation and computer simulation will be used for evaluation of the performance of the proposed technique.
Abstract 2307: Elucidation of a unique regulatory mechanism for TNK1 provides potential therapeutic targeting opportunities in cancer (2021)

Abstract Sumitomo Dainippon Pharma Oncology (f/k/a Tolero Pharmaceuticals), Lehi, UTTNK1 is a poorly understood member of the ACK family of non-receptor tyrosine kinases with a unusual domain arrangement, but no known mechanism of regulation nor conclusive link to disease. We initially identified TNK1 as a mediator of cell survival in a subset of primary patient cancer samples. In an effort to understand how TNK1 is regulated, we discovered a MARK-mediated phosphorylation at S502 near the TNK1 C-terminus that mediates an interaction with 14-3-3. We found that 14-3-3 binding inhibitsTNK1 kinase activity, whereas disruption of this interaction renders TNK1 highly active and capable of driving tumor growth in vivo. In support of this idea, kinase substrate profiling at the proteome level revealed a variety of pro-growth and motility substrates for active TNK1. One unique feature of TNK1 is a ubiquitin-association domain (UBA) on its C-terminus, which sits adjacent to the 14-3-3 binding site. We found that the TNK1 UBA has a high affinity for a variety of poly-ubiquitin linkages—making TNK1the first kinase, to our knowledge, that directly interacts (non-covalently) with ubiquitin. Remarkably, point mutations within the UBA that disrupt ubiquitin binding inhibit TNK1 activation and oncogenic signaling, revealing a unique UBA-centric mechanism of tyrosine kinase regulation, in which TNK1toggles between 14-3-3-bound (inactive) and ubiquitin-bound (active) states. Finally, we used a structure-guided approach to identify novel small molecule TNK1 inhibitors with high potency and selectivity. One such compound, TP-5809, inhibits TNK1 dependent STAT5 phosphorylation in vitro and in vivo in L540, a Hodgkin lymphoma cell line with a TNK1-activating mutation. TP-5809 also potently inhibits the in vitro growth of mutant TNK1-driven pro-B cells and reduces disease burden and prolongs survival in mice with xenografted tumors. Together, our data elucidate the first mechanism of TNK1regulation and identify lead compounds for the development of a TNK1 inhibitor. Citation Format: Tsz-Yin Chan, Christina Egbert, Logan Larsen, Jeremy Tsang, Julia Maxson, Eranga Roshan, Clifford J. Whatcott, Kim Wontak, Gaelle Mercenne, Savannah Free, Adam Siddiqui, Tetyana Forostyan, Ethika Tyagi, Kenneth A. Christensen, David J. Bearss, James Moody, Jeffrey Tyner, Jason M. Foulks, Steven L. Warner, Joshua Lyon Andersen. Elucidation of a unique regulatory mechanism for TNK1 provides potential therapeutic targeting opportunities in cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2021; 2021 Apr 10-15 and May 17-21. Philadelphia (PA): AACR; Cancer Res 2021;81(13_Suppl):Abstract nr 2307.
Transport Phenomena in Polymer Processing (2017)

No AccessUnderstanding Polymer ProcessingOct 2017Transport Phenomena in Polymer ProcessingTim A. OsswaldTim A. OsswaldSearch for more papers by this authorhttps://doi.org/10.3139/9781569906484.009SectionsAboutPDF ToolsAdd to FavoritesDownload CitationTrack CitationsCopy LTI LinkPDF key 'share (en)' returned an object instead of string.FacebookTwitterEmailLinkedIn previous chapternext chapter FiguresReferencesRelatedDetails 2017Pages: 203-257Print ISBN: 978-1-56990-647-7eISBN: 978-1-56990-648-4 Copyright & Permissions© 2017 Carl Hanser Verlag GmbH & Co. KGPDF downloadLoading ...