gebhart/patrepeval huggingface dataset:
Immunoglobulin VH gene expression in human B cell lines and tumors: biased VH gene expression in chronic lymphocytic leukemia (1989)

We have studied frequencies of VH gene utilization in a panel of monoclonal Epstein-Barr virus (EBV)-transformed B cell lines derived from human adult and fetal tissues as well as in monoclonal B cells obtained from fresh chronic lymphocytic leukemia (CLL) samples. The results show that IgM-secreting EBV cell lines from both fetal and adult tissues utilize VH genes from particular families roughly in proportion to estimated family size, suggesting that the repertoire of sigM-positive B cells in both fetal and adult organs is 'normalized' with respect to the V(H) gene family. In contrast, we find a highly biased pattern of VH gene expression in CLLs. The significance of these findings is discussed in the context of mechanisms that could be involved in normal B cell repertoire development and in the process of malignant transformation of precursors of CLL.
Human melanoma cells do not express Fas (Apo-1/CD95) ligand. (1999)

A recent report described the expression of Fas ligand (FasL) by melanoma cells as an important mechanism involved in the immune evasion by tumors [M. Hahne et al., Science (Washington DC), 274: 1363-1366, 1996]. To investigate the expression of FasL by melanomas, we screened a panel of early-passage cell lines by functional assay and reverse transcriptase-PCR. Using conditions designed to replicate those in the original report, we did not find functional FasL on any of the 19 human melanoma lines established at the National Cancer Institute. Furthermore, we additionally evaluated our melanoma lines using reverse transcriptase-PCR and found that 0 of the 26 human melanoma cell lines expressed FasL mRNA. FasL mRNA was abundantly expressed by anti-melanoma T-cell lines after activation. These data do not support a role for FasL expression in the escape of melanoma cells from immune destruction.
Two-Cell MISO Interfering Broadcast Channel with Limited Feedback: Adaptive Feedback Strategy and Multiplexing Gains (2011)

In this paper, we study a two cell multiple input single-output interfering broadcast channel with finite rate feedback. In this system, we first derive the rate loss due to the quantization error by considering a coordinated zero-forcing beamforming. In addition, feedback bits allocation methods are proposed to minimize the performance degradation caused by the quantization error. Lastly, we investigate how many feedback bits per user are necessary to maintain the optimal multiplexing gain in MISO-IFBC. Through numerical evaluations, we show that our proposed feedback bits allocation strategy provides significant gain compared to a trivial bits allocation scheme.
Antisense oligonucleotide-induced alternative splicing of the APOB mRNA generates a novel isoform of APOB (2007)

Background: Apolipoprotein B (APOB) is an integral part of the LDL, VLDL, IDL, Lp(a) and chylomicron lipoprotein particles. The APOB pre-mRNA consists of 29 constitutively-spliced exons. APOB exists as two natural isoforms: the full-length APOB100 isoform, assembled into LDL, VLDL, IDL and Lp(a) and secreted by the liver in humans; and the C-terminally truncated APOB48, assembled into chylomicrons and secreted by the intestine in humans. Down-regulation of APOB100 is a potential therapy to lower circulating LDL and cholesterol levels.Results: We investigated the ability of 2' O-methyl RNA antisense oligonucleotides (ASOs) to induce the skipping of exon 27 in endogenous APOB mRNA in HepG2 cells. These ASOs are directed towards the 5' and 3' splice-sites of exon 27, the branch-point sequence (BPS) of intron 26-27 and several predicted exonic splicing enhancers within exon 27. ASOs targeting either the 5' or 3' splice-site, in combination with the BPS, are the most effective. The splicing of other alternatively spliced genes are not influenced by these ASOs, suggesting that the effects seen are not due to non-specific changes in alternative splicing. The skip 27 mRNA is translated into a truncated isoform, APOB87(SKIP27).Conclusion: The induction of APOB87(SKIP27) expression in vivo should lead to decreased LDL and cholesterol levels, by analogy to patients with hypobetalipoproteinemia. As intestinal APOB mRNA editing and APOB48 expression rely on sequences within exon 26, exon 27 skipping should not affect APOB48 expression unlike other methods of down-regulating APOB100 expression which also down-regulate APOB48.
Time series analysis and segmentation using eigenvectors for mining semantic audio label sequences (2005)

Pattern discovery from video has promising applications in summarizing different genre types, including surveillance and sports. After pattern discovery, a summary of the video can be constructed from a combination of usual and unusual patterns, depending on the application domain. Previously, we used an unsupervised label mining approach to extract highlight moments from soccer videos (Radhakrishan, R. et al., IEEE Pacific-Rim Conf. on Multimedia, 2003). We now formulate the problem of pattern discovery from semantic audio labels as a time series clustering problem and propose a new unsupervised mining framework based on segmentation theory using eigenvectors of the affinity matrix. We test the validity of the technique using synthetically generated label sequences as well as label sequences from broadcast sports video. Our sports highlights extraction accuracy is comparable to that achieved in our previous work.
2013 R&D 100 Award: New tech could mean more power for fiber lasers (2014)

An LLNL team of six physicists has developed a new technology that is a stepping stone to enable some of the limitations on high-power fiber lasers to be overcome. Their technology, dubbed "Efficient Mode-Converters for High-Power Fiber Amplifiers," allows the power of fiber lasers to be increased while maintaining high beam quality. Currently, fiber lasers are used in machining, on factory floors and in a number of defense applications and can produce tens of kilowatts of power.The conventional fiber laser design features a circular core and has fundamental limitations that make it impractical to allow higher laser power unless the core area is increased. LLNL researchers have pioneered a design to increase the laser's core area along the axis of the ribbon fiber. Their design makes it difficult to use a conventional laser beam, so the LLNL team converted the beam into a profile that propagates into the ribbon fiber and is converted back once it is amplified. The use of this LLNL technology will permit the construction of higher power lasers for lower costs and increase the power of fiber lasers from tens of kilowatts of power to about 100 kilowatts and potentially even higher.
Pharmakokinetik und Biotransformation neuer Röntgenkontrastmittel für die Uro- und Angiographie beim Patienten (1977)

Pharmakokinetik und Biotransformation der bekannten Röntgenkontrastmittel Amidotrizoat und Iothalamat wurden im Vergleich zu den Prüfsubstanzen Hydroxyamidotrizoat bzw. den Aminosäuren enthaltenden Kontrastmitteln Ioglicinat und Ioserinat an Patienten untersucht. Die Proteinbindung der Kontrastmittel wurde in vitro bestimmt. 1. Das Verteilungsvolumen betrug für alle Kontrastmittel einheitlich 18 + 2% des Körpergewichts. Die Halbwertszeit im Plasma war für die Ioglicinsäure mit 88 ± 13 min am kürzesten und für die Hydroxyamidotrizoesäure mit 121 ± 21 min am längsten. 2. Alle Substanzen wurden zu ≥ 85% mit dem Harn ausgeschieden. Bis 30 min p. inj. wurden 10–15% der Dosis, bis 3 h p. inj. etwa 50% der Dosis im Harn wiedergefunden. Die Ausscheidung des Hydroxyamidotrizoat verlief am langsamsten. 3. In Blut und Harn konnten nach Injektion der angeführten Kontrastmittel in keinem Falle Metabolite oder freies Jodid nachgewiesen werden. 4. Die Prüfsubstanzen Hydroxyamidotrizoat, Ioglicinat und Ioserinat wurden in noch geringerem Ausmaß an die Proteine des Plasmas gebunden als Amidotrizoat und Iothalamat. Besonders die Ioglicinsäure scheint aufgrund ihrer Pharmakokinetik für die Urographie geeignet.
Effect of exercise on myocardial energy metabolism and relationship between coronary microvascular dysfunction and abnormal myocardial energetics in diabetic cardiomyopathy (2015)

Background Diabetic cardiomyopathy is a well-recognised entity, with multifactorial aetiology. We and others have previously shown impaired myocardial energetics (decreased PCr/ ATP using cardiac 31P Magnetic Resonance Spectroscopy-MRS) in patients with type II diabetes mellitus (T2DM) at rest. Furthermore, diabetic patients are known to have impaired myocardial perfusion reserve even in the absence of epicardial coronary artery disease likely due to microvascular dysfunction. However, it is unknown whether this hypoperfusion is translated to further energetic derangement at stress. Methods 27 patients (13 male, mean age 54±1.5 years; BMI 28±2.7) with T2DM and 24 healthy volunteers of similar age and BMI (13 male, mean age 51±2.6 years; BMI 26±0.84) were studied. Patients were on oral antidiabetic therapies only, with mean HBA1c 7.4±1.3% and no history of diabetic complications. Obstructive coronary artery disease (defined as> 50% lumen diameter reduction) was excluded in all patients by CT coronary angiography. Cardiac 31PMRS (3T) was performed at rest and during 8 minutes of leg exercise lying prone, with 2.5 kg weights attached to both legs. First-pass perfusion images (using a saturation recovery fast-gradient echo sequence and 0.03 mmol/kg bolus of Gadoterate meglumine (Dotarem, Guerbet LLC, France ) were also acquired at stress (3-6 minutes i.v. adenosine, 140 μg/kg/min) and rest at mid ventricular short axis. myocardial perfusion reserve index (MPRI) were measured from perfusion images. Results Left ventricular ejection fraction (LVEF) and mass index (LVMI) were similar in patients with T2DM and healthy volunteers (LVEF 70±1 vs 71±0.9%, p=0.494; LVMI 56.7 ±1.8 vs 52.6±2.4g/m 2 , p=0.168). Increases in rate pressure product with exercise (T2DM 46±36%, healthy volunteers 42±26%, P = 0.69) and adenosine stress (T2DM 40 ± 3%, healthy volunteers 39 ± 3%, P = 0.89) were similar. There was no change in PCr/ATP during exercise in healthy volunteers (rest: 2.03±0.05, exercise:
Innovative Two-Part Impression Technique for an Extensive Maxillary Defect. (2015)

Patients reporting with large maxillary defects pose great difficulty in prosthodontic practice. After thorough history and clinical examination, prosthetic rehabilitation of these patients usually starts with an impression. In such cases conventional single step impression technique solely may not give the desired results. If the defect is of large size then impression making becomes difficult and complicated procedure. Therefore, conventional impression technique can be modified according to the needs and requirements. This report describes a modified and innovative technique of making impression in two parts, which can be withdrawn from mouth in two segments and easily reoriented outside the mouth.
Homogeneous Oxidative Coupling Catalysts (1979)

The products of oxidation of Cu(I) chloride by oxygen in aprotic ligand/solvent systems are useful catalysts for a variety of oxidative coupling reactions of molecular oxygen. The ligand/solvent environment determines the nature of the products. The copper-reduced oxygen products obtained in pyridine are growing polymers with a pyridinestabilized CuO core, which can be separated from the py2-CuCl2 co-product, and are the initiators for the oxidative coupling of phenols. Saturated, methylated amines are ineffective stabilizers of the copper-reduced oxygen interaction while amide and lactam ligands stabilize distinct clustered primary oxidation products. Cryoscopic measurements and a determination of the structure of a catalyst derivative with coordinated N-methyl-2-pyrrolidinone ligands are valuable in furthering our understanding of the structure of the products formed in other ligand/solvent systems.
Techno-economic Assessment for Integrating Biosorption into Rare Earth Recovery Process (2017)

The current uncertainty in the global supply of rare earth elements (REEs) necessitates the development of novel extraction technologies that utilize a variety of REE source materials. Herein, we examined the techno-economic performance of integrating a biosorption approach into a large-scale process for producing salable total rare earth oxides (TREOs) from various feedstocks. An airlift bioreactor is proposed to carry out a biosorption process mediated by bioengineered rare earth-adsorbing bacteria. Techno-economic assessments were compared for three distinctive categories of REE feedstocks requiring different pre-processing steps. Key parameters identified that affect profitability include REE concentration, composition of the feedstock, and costs of feedstock pretreatment and waste management. Among the 11 specific feedstocks investigated, coal ash from the Appalachian Basin was projected to be the most profitable, largely due to its high-value REE content. Its cost breakdown includes pre-processing (leaching primarily, 77.1%), biosorption (19.4%), and oxalic acid precipitation and TREO roasting (3.5%). Surprisingly, biosorption from the high-grade Bull Hill REE ore is less profitable due to high material cost and low production revenue. Overall, our results confirmed that the application of biosorption to low-grade feedstocks for REE recovery is economically viable.
Supplementary Table S3 from A very long-acting exatecan and its synergism with DNA damage response inhibitors (2023)

&lt;p&gt;Synergy of PEG-Exa 3A and the ATRi VX-970.&lt;/p&gt;
High-Quality Synthetic Diamond Crystals. (1998)

High-quality synthetic type IIa diamond crystals of several carats, containing less than 0. 1 ppm chemical impurities and few inclusions, have been successfully synthesized by the temperature gradient method under high pressure and high temperature. The crystals are transparent over a wide range of wavelengths from ultraviolet to far infrared regions, showing no absorptions due to impurities. They also have high crystalline quality with fewer crystal defects, less internal strain, and less variation in defects among crystals than those of natural diamonds or conventional synthetic type Ib diamond. The outstanding characteristics of the synthetic type IN diamond permit application to a considerably wide range of industrial and scientific uses such as optical parts, monochromators, high-pressure anvils, and so on.
Matrix elasticity of void-forming hydrogels controls transplanted-stem-cell-mediated bone formation (2015)

The effectiveness of stem cell therapies has been hampered by cell death and limited control over fate. These problems can be partially circumvented by using macroporous biomaterials that improve the survival of transplanted stem cells and provide molecular cues to direct cell phenotype. Stem cell behaviour can also be controlled in vitro by manipulating the elasticity of both porous and non-porous materials, yet translation to therapeutic processes in vivo remains elusive. Here, by developing injectable, void-forming hydrogels that decouple pore formation from elasticity, we show that mesenchymal stem cell (MSC) osteogenesis in vitro, and cell deployment in vitro and in vivo, can be controlled by modifying, respectively, the hydrogel’s elastic modulus or its chemistry. When the hydrogels were used to transplant MSCs, the hydrogel’s elasticity regulated bone regeneration, with optimal bone formation at 60 kPa. Our findings show that biophysical cues can be harnessed to direct therapeutic stem cell behaviours in situ.
Combination of 5-flucytosine and capsule-binding monoclonal antibody in the treatment of murine Cryptococcus neoformans infections and in vitro (1996)

The efficacy of combination therapy with 5-flucytosine and an IgG1 monoclonal antibody to Cryptococcus neoformans capsular glucuronoxylomannan was studied in vitro with J774. 16 murine macrophage-like cells and in vivo in murine cryptococcal infection. The combination of 5-flucytosine and IgG1 was more effective in reducing the numbers of C. neoformans colony-forming units in vitro and in vivo than either agent alone.
Genetically Engineered Lactococcus lactis Secreting Murine IL‐10 Modulates the Functions of Bone Marrow‐Derived Dendritic Cells in the Presence of LPS (2008)

Oral delivery of IL-10 by genetically modified Lactococcus lactis (LL-pTmIL10) has been shown to efficiently reduce intestinal inflammation in mice with chronic colitis, but the mechanisms involved have not been elucidated. It has been suggested that IL-10 controls intestinal inflammation by inhibiting microbe-induced activation of dendritic cells. We therefore investigated whether LL-pTmIL10 can modulate the functions of bone marrow-derived dendritic cells (BM-DC) responding to LPS. Incubation of these cells with LL-pTmIL10 or with the control strain LL-pTREX reduced their ability to activate allogeneic T-cell proliferation. However, in contrast to LL-pTREX, LL-pTmIL10 inhibited the LPS-stimulated secretion of MCP-1 by BM-DC and reduced the synergistic up-regulation of IL-12/IL-23p40. In addition, LL-pTmIL10 treatment of LPS-stimulated BM-DC significantly inhibited their capacity to induce strong secretion of IL-17 by CD4+ T cells. Our data suggest that the beneficial effects of LL-pTmIL10 treatment during chronic colitis might involve inhibition of CD4+ Th17 cells and a reduced accumulation of these cells as well as other immune cells at the site of inflammation.
The Blood And Clot Thrombectomy Registry And Collaboration (BACTRAC) protocol: novel method for evaluating human stroke (2018)

We have developed a novel tissue banking protocol using mechanical thrombectomy to capture thrombus along with arterial blood proximal and distal to it. The protocol provides high-quality specimens, facilitating analysis of the initial molecular response to ischemic stroke in the human condition for the first time. This approach will permit reverse translation to animal models for treatment development.
265: A Constitutively Activated Mutant form of Propsa that Manifests Catalytic Action Increases Cell Invasion in Vitro and Frequency of Pulmonary Metastases in vivo in a Subcutaneous Mouse Xenograft Model (2007)

You have accessJournal of UrologyModerated Poster 10, Sunday, May 20, 2007, 10:00 am - 12:00 pm1 Apr 2007265: A Constitutively Activated Mutant form of Propsa that Manifests Catalytic Action Increases Cell Invasion in Vitro and Frequency of Pulmonary Metastases in vivo in a Subcutaneous Mouse Xenograft Model Sven Wenske, Todd Hricik, Brett S. Carver, Matthew F. O'Brien, Ruslan Korets, Carlos Cordon-Cardo, Pier-Paolo Pandolfi, Glenn Heller, Howard I. Scher, James A. Eastham, Peter T. Scardino, and Hans G. Lilja Sven WenskeSven Wenske , Todd HricikTodd Hricik , Brett S. CarverBrett S. Carver , Matthew F. O'BrienMatthew F. O'Brien , Ruslan KoretsRuslan Korets , Carlos Cordon-CardoCarlos Cordon-Cardo , Pier-Paolo PandolfiPier-Paolo Pandolfi , Glenn HellerGlenn Heller , Howard I. ScherHoward I. Scher , James A. EasthamJames A. Eastham , Peter T. ScardinoPeter T. Scardino , and Hans G. LiljaHans G. Lilja View All Author Informationhttps://doi.org/10.1016/S0022-5347(18)30530-5AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "265: A Constitutively Activated Mutant form of Propsa that Manifests Catalytic Action Increases Cell Invasion in Vitro and Frequency of Pulmonary Metastases in vivo in a Subcutaneous Mouse Xenograft Model." The Journal of Urology, 177(4S), p. 89 © 2016 by American Urological AssociationFiguresReferencesRelatedDetails Volume 177Issue 4SApril 2007Page: 89 Advertisement Copyright & Permissions© 2016 by American Urological AssociationMetricsAuthor Information Sven Wenske More articles by this author Todd Hricik More articles by this author Brett S. Carver More articles by this author Matthew F. O'Brien More articles by this author Ruslan Korets More articles by this author Carlos Cordon-Cardo More articles by this author Pier-Paolo Pandolfi More articles by this author Glenn Heller More articles by this author Howard I. Scher More articles by this author James A. Eastham More articles by this author Peter T. Scardino More articles by this author Hans G. Lilja More articles by this author Expand All Advertisement PDF downloadLoading ...
The interaction of twisted Laguerre-Gaussian light with a GaAs photocathode to investigate photogenerated polarized electrons (2019)

The interaction of Laguerre-Gaussian light at selected wavelengths from 690 to 810 nm with a p-type gallium arsenide (GaAs) photocathode photonic device and the production of photogenerated electrons were investigated. Spin angular momentum (SAM) and orbital angular momentum (OAM) were generated using linear or circular polarization and q-plates. The degree of polarization P from the photogenerated electron signal was measured. At an excitation of 695 nm (E = 1.78 eV), POAM = 2.1%, PSAM = 1.3%, and PSAM,OAM(q=1)=3.4%, whereas, at 800 nm (E = 1.55 eV), near the bandgap, POAM = −14.7%, PSAM = −2.6%, and PSAM,OAM(q=1)=4.0%.
Multiple epiphyseal dysplasia. Report of a pedigree. (1987)

Three generations of one family with multiple epiphyseal dysplasia were evaluated with identification of three affected individuals. One patient treated by transtrochanteric anterior rotational osteotomy of the femoral head for osteoarthritis of the hip was reported.
Correction: Corrigendum: Abiotic ligation of DNA oligomers templated by their liquid crystal ordering (2015)

Nature Communications 6: Article number: 6424 (2015); Published 10 March 2015; Updated 17 June 2015 The original version of this Article contained an error in the spelling of the author Youngwoo Yi, which was incorrectly given as Yougwooo Yi. This has now been corrected in both the PDF and HTML versions of the Article.
Association of xylem-limited bacteria with elm, sycamore, and oak leaf scorch (1980)

Ultrathin sections of leaves from American elms (Ulmus americana L.), sycamores (Platanus spp.), and two red oak species (Quercus spp.) that exhibited leaf scorch were examined by transmission electron microscopy. Rod-shaped, ripple-walled bacteria resembling the Pierce's disease organism were found consistently in tracheary elements of the primary and secondary veins of diseased plants. Smaller, ripple-walled, densely stained, irregular-shaped bodies (SDB) were found also in a matrix that lined the inner walls or filled the lumina of the tracheary elements. In leaf scorch affected American elms the bacteria were 0.3–0.4 μrn × 0.9–2.4 μm, with rounded ends. Fimbriae-like structures radiated from a few organisms. Bacteria were frequently embedded in a matrix. Pit cavities and the ends of tracheary elements were often filled with the bacteria–matrix complex. Rod-shaped bacteria were not as numerous in the diseased sycamore and oak as in diseased elm; however, SDB's were more numerous. Bacteria in sycamore (1.0–1.8 μm) and oak (1.0–2.0 μm) were slightly shorter than those in elm, many had numerous fimbriae-like hairs, and some were tapered at one end. Indirect fluorescent antibody staining showed a serological relationship between bacteria extracted from elm and oak and the Pierce's disease bacterium.