MP84-18 NON-ALCOHOLIC FATTY LIVER DISEASE AND ERECTILE DYSFUNCTION IN S-NITROSOGLUTATHIONE REDUCTASE (GSNOR) DEFICIENCY (2020)

You have accessJournal of UrologySexual Function/Dysfunction: Basic Research & Pathophysiology (MP84)1 Apr 2020MP84-18 NON-ALCOHOLIC FATTY LIVER DISEASE AND ERECTILE DYSFUNCTION IN S-NITROSOGLUTATHIONE REDUCTASE (GSNOR) DEFICIENCY Dorota Hawksworth*, Justin La Favor, Robert Andres, Claire Kuo, Jennifer Cullen, Trinity Bivalacqua, and Arthur Burnett Dorota Hawksworth*Dorota Hawksworth* More articles by this author , Justin La FavorJustin La Favor More articles by this author , Robert AndresRobert Andres More articles by this author , Claire KuoClaire Kuo More articles by this author , Jennifer CullenJennifer Cullen More articles by this author , Trinity BivalacquaTrinity Bivalacqua More articles by this author , and Arthur BurnettArthur Burnett More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000000976.018AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Recent animal and human studies link non-alcoholic fatty liver disease(NAFLD) to male sexual dysfunction. The molecular and physiologic mechanisms correlating these conditions are incompletely established at this time. Since GSNOR is an important liver enzyme protecting against Western diet (WD)-induced erectile dysfunction (ED), we examined its potential concomitant effects on development and progression of NAFLD. METHODS: Male wild type and GSNOR-/-(mutant) mice were fed either a control diet (CD) or a WD (high fat and sugar content) for 3, 6, 9 or 12 weeks. All mice were studied at 20 weeks of age. Intra-cavernosal pressure (ICP) was measured at 1, 2, and 4 Volts electrical stimulation. Once mice were sacrificed at end of experiments, liver tissue was procured and preserved for histological examination. H&E-stained slides were blindly examined by an experienced liver pathologist and scored based on presence and extent of steatosis, lobular inflammation and hepatocyte necrosis. RESULTS: Electrical stimulation induced ICP responses revealed no difference in erectile function between WT and GSNOR-/-mice fed the CD. At nine weeks of WD, all animals developed ED, however those with GSNOR deficiency had a significantly worsened ICP values (p-value = 0.05). GSNOR-/-mice fed CD had worse liver scores at baseline, when compared to wild type animals, irrespective of the diet type. The liver dysfunction score in GSNOR-/-mice doubled following 9 weeks of WD. CONCLUSIONS: This study demonstrates a likely relationship of NAFLD and ED in animals fed Western Diet. Furthermore, GSNOR deficiency appears to be an important factor predisposing to both liver and erectile dysfunction, regardless of the dietary choices. Additional work is necessary to fully examine underlying complex molecular relationships as they relate to NAFLD and ED development and progression. Source of Funding: None © 2020 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 203Issue Supplement 4April 2020Page: e1271-e1271 Advertisement Copyright & Permissions© 2020 by American Urological Association Education and Research, Inc.MetricsAuthor Information Dorota Hawksworth* More articles by this author Justin La Favor More articles by this author Robert Andres More articles by this author Claire Kuo More articles by this author Jennifer Cullen More articles by this author Trinity Bivalacqua More articles by this author Arthur Burnett More articles by this author Expand All Advertisement PDF downloadLoading ...