gebhart/patrepeval huggingface dataset:
<em>In vivo</em> <sup>19</sup>F MRI for Cell Tracking (2013)

In vivo 19F MRI allows quantitative cell tracking without the use of ionizing radiation. It is a noninvasive technique that can be applied to humans. Here, we describe a general protocol for cell labeling, imaging, and image processing. The technique is applicable to various cell types and animal models, although here we focus on a typical mouse model for tracking murine immune cells. The most important issues for cell labeling are described, as these are relevant to all models. Similarly, key imaging parameters are listed, although the details will vary depending on the MRI system and the individual setup. Finally, we include an image processing protocol for quantification. Variations for this, and other parts of the protocol, are assessed in the Discussion section. Based on the detailed procedure described here, the user will need to adapt the protocol for each specific cell type, cell label, animal model, and imaging setup. Note that the protocol can also be adapted for human use, as long as clinical restrictions are met.
Evaluating twenty-years of follow-up after orthotopic liver transplantation, best practice for donor-recipient matching: What can we learn from the past era? (2016)

LT offers excellent long-term results in case of optimal donor and recipient conditions. However, mainly due to the current organ shortage, these ideal circumstances are rarely given; thus algorithms for donor-recipient matching need to be refined, in order to enable a maximum benefit for the recipients of high quality as well as marginal organs.
Optical fiber sensors (1988)

Optical Fiber-Chemical Sensing Using Direct Spectroscopy. Chemical Sensing Using Indicator Dyes. Dynamic Light Scattering Applied and Its Application in Concentrated Suspensions. In Vivo Medical Sensors. Fiber-Optic Gyros. Fiber-Optic Sensors for Condition Monitoring and Engineering Diagnostics. Sensors in Industrial Systems. Distributed Sensors -- Recent Developments. Multiplexing Techniques for Fiber-Optic Sensors. Fiber Optics and Smart Structures. Fiber-Optic Sensors -- Commercial Presence.
Prevalence and Determinants of Iron Deficiency in Cardiac Amyloidosis (2022)

Iron deficiency is common in patients with CA, irrespective of the subtype. Patients seem more likely to have ID if diagnosed with ATTR, if diabetic, and/or treated with aspirin. In CA, the benefit of intravenous iron therapy, for ID, on morbidity and mortality needs further study.
Prediction of Pneumococcal Conjugate Vaccine Effectiveness against Invasive Pneumococcal Disease Using Opsonophagocytic Activity and Antibody Concentrations Determined by Enzyme-Linked Immunosorbent Assay with 22F Adsorption (2011)

ABSTRACT We compared the abilities of two serological readouts, antipolysaccharide IgG antibody concentrations and opsonophagocytic activity (OPA) titers, to predict the clinical effectiveness of the 7-valent pneumococcal conjugate vaccine (7vCRM) against invasive pneumococcal disease (IPD). We also assessed the accuracy of the previously established thresholds for GlaxoSmithKline's enzyme-linked immunosorbent assay with 22F adsorption (22F-ELISA) (≥0.2 μg/ml) and OPA assay (titer, ≥8) in predicting effectiveness. We showed that following a 3-dose 7vCRM primary vaccination, the serological response rates as determined using thresholds of ≥0.2 μg/ml IgG and an OPA titer of ≥8 corresponded well with overall effectiveness against IPD. In addition, the OPA assay seemed to better predict serotype-specific effectiveness than enzyme-linked immunoassay. Finally, when applied to post-dose-2 immune responses, both thresholds also corresponded well with the overall IPD effectiveness following a 2-dose 7vCRM primary vaccination. These results support the importance of the OPA assay in evaluating immune responses to pneumococcal conjugate vaccines.
Changes in Dopamine D<sub>2</sub> Receptors and 6-[<sup>18</sup>F]Fluoro-<i>L</i>-3,4-Dihydroxyphenylalanine Uptake in the Brain of 6-Hydroxydopamine- Lesioned Rats (2004)

We studied tracer distributions in positron emission tomography of ligands for dopamine D1 receptors ([11C]SCH23390) and D2 receptors ([11C]raclopride) and the dopamine precursor analog 6-[18F]fluoro-L-3,4-dihydroxyphenylalanine ([18F]FDOPA), as a measurement of presynaptic dopaminergic function, in the brain after 6-hydroxydopamine lesioning of the medial forebrain bundle in rats. The unilateral lesions were confirmed behaviorally by methamphetamine-induced rotation 2 weeks after lesioning, and the brains were analyzed by tissue dissection following an intravenous bolus of each tracer 3 weeks after lesioning. [11C]Raclopride, but not [11C]SCH23390, showed a higher accumulation in the striatum on the lesion side compared with that on the non-lesioned (intact) side. On the other hand, a lower accumulation of [18F]FDOPA was found in the striatum and cerebral cortex on the lesion side. Our studies demonstrate upregulation of dopamine D2 receptors in the striatum and a decrease in FDOPA uptake in both the striatum and cerebral cortex ipsilateral to the 6-hydroxydopamine lesions. Therefore, the combination of a D2 antagonist and FDOPA may provide a potentially useful method for assessing the effects of dopamine depletion in Parkinson's disease.
Faculty Opinions recommendation of Copper-phthalocyanine conjugates of serum albumins as enantioselective catalysts in Diels-Alder reactions. (2006)

No abstract available PMID: 16528766
PRODUCTION OF TL ANTIBODY BY MICE IMMUNIZED WITH TL- CELL POPULATIONS (1973)

TL(-) mice make TL antibody when immunized with spleen or bone marrow cells from TL(+) donors, despite the fact that these cells do not express TL antigen. This has been shown to depend on maturation of TL(-) precursors, contained in the inoculum, into TL(+) cells under influence of the recipient's thymus; the differentiated TL(+) cells then evoke production of TL antibody.
ChemInform Abstract: A New P‐Chiral Bisphosphine, 1,1′‐Bis[(t‐butyl)methylphosphino]ferrocene, as an Effective Ligand in Catalytic Asymmetric Hydrosilylation of Simple Ketones. (1999)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Structural insights into the inhibition mechanism of human sterol O-acyltransferase 1 by a competitive inhibitor (2020)

Sterol O-acyltransferase 1 (SOAT1) is an endoplasmic reticulum (ER) resident, multi-transmembrane enzyme that belongs to the membrane-bound O-acyltransferase (MBOAT) family. It catalyzes the esterification of cholesterol to generate cholesteryl esters for cholesterol storage. SOAT1 is a target to treat several human diseases. However, its structure and mechanism remain elusive since its discovery. Here, we report the structure of human SOAT1 (hSOAT1) determined by cryo-EM. hSOAT1 is a tetramer consisted of a dimer of dimer. The structure of hSOAT1 dimer at 3.5 Å resolution reveals that a small molecule inhibitor CI-976 binds inside the catalytic chamber and blocks the accessibility of the active site residues H460, N421 and W420. Our results pave the way for future mechanistic study and rational drug design targeting hSOAT1 and other mammalian MBOAT family members.
Application of Nano-indentation method to characterize adhesion strength of polyimide film (2019)

Thick polyimide layer has been used as buffer layer to absorb stress imposed by molding compounds for many years. This paper studied the adhesion strength of the thick polyimide layer under different process conditions. The stress was induced by indentation, and delamination was confirmed by FIB and SEM imaging. The calculated adhesion energy for polyimide film was highest, around 30 J/m2, for the wafer processed with an extra pad protection layer Nblok (Nitrogen-doped barrier on top of copper, SiCN) plasma clean before the spin coating of polyimide film.
Google's cross-dialect Arabic voice search (2012)

We present a large scale effort to build a commercial Automatic Speech Recognition (ASR) product for Arabic. Our goal is to support voice search, dictation, and voice control for the general Arabic-speaking public, including support for multiple Arabic dialects. We describe our ASR system design and compare recognizers for five Arabic dialects, with the potential to reach more than 125 million people in Egypt, Jordan, Lebanon, Saudi Arabia, and the United Arab Emirates (UAE). We compare systems built on diacritized vs. non-diacritized text. We also conduct cross-dialect experiments, where we train on one dialect and test on the others. Our average word error rate (WER) is 24.8% for voice search.
Active Patellar Tracking Measurement (2004)

This novel method for measurement of dynamic patellar mediolateral tracking was found to have good intraobserver and interobserver reproducibility, and the measurements matched closely with those obtained from magnetic resonance imaging reconstructions of static patellar positions. Some preliminary data for tracking in 3 activities were obtained from 10 normal knees.
<title>Device properties and polar anchoring of nematic molecules at photodimerized surfaces</title> (1999)

A photo-dimerized monolayer (PDML) has been recently developed by us for liquid crystal alignment. Electro-optic properties of a single pixel prototype device with the PDML alignment layer have been measured. Data on the contrast, threshold voltage and slope of the response curve are presented which demonstrate the efficacy of the PDML layer for LCD applications. In addition, the polar anchoring energy was measured for PDML layers differing in the chemical composition. The ability to tune this anchoring energy through chemical modification is demonstrated. It is shown that the anchoring strength is comparable to that obtained for liquid crystal cells using the mechanically rubbed polyimide alignment layer.
Research on Another Permanent Data Storage Solution (2012)

This paper has provided details on the LE of the data on magnetic tape, hard-disc drives, flash memory, and optical discs. For archival purposes, only the M-DISC presently meets the requirement of long-term data retention (more than 100 years). This disc has been rigorously tested, and the results are very positive.The main weakness of the M-DISC is its relatively small capacity. This weakness is being addressed by our current research on a permanent solid-state solution, one whose density will enable a capacity of several terabytes in the format of a solid-state drive. Though it will be much more expensive than an M-DISC, the convenience of having several terabytes in a single, removable form of storage should be very attractive to the archival community.
Multiscale Manifold Warping (2021)

Many real-world applications require aligning two temporal sequences, including bioinformatics, handwriting recognition, activity recognition, and human-robot coordination. Dynamic Time Warping (DTW) is a popular alignment method, but can fail on high-dimensional real-world data where the dimensions of aligned sequences are often unequal. In this paper, we show that exploiting the multiscale manifold latent structure of real-world data can yield improved alignment. We introduce a novel framework called Warping on Wavelets (WOW) that integrates DTW with a a multi-scale manifold learning framework called Diffusion Wavelets. We present a theoretical analysis of the WOW family of algorithms and show that it outperforms previous state of the art methods, such as canonical time warping (CTW) and manifold warping, on several real-world datasets.
Radioanalytical Chemistry for Automated Nuclear Waste Process Monitoring (2003)

This research program is directed toward rapid, sensitive, and selective determination of beta and alpha-emitting radionuclides such as 99Tc, 90Sr, and trans-uranium (TRU) elements in low activity waste (LAW) processing streams. The overall technical approach is based on automated radiochemical measurement principles. Nuclear waste process streams are particularly challenging for rapid analytical methods due to the complex, high- ionic-strength, caustic brine sample matrix, the presence of interfering radionuclides, and the variable and uncertain speciation of the radionuclides of interest. As a result, matrix modification, speciation control, and separation chemistries are required for use in automated process analyzers. Significant knowledge gaps exist relative to the design of chemistries for such analyzers so that radionuclides can be quantitatively and rapidly separated and analyzed in solutions derived from low-activity waste processing operations. This research is addressing these knowledge gaps in the area of separation science, nuclear detection, and analytical chemistry and instrumentation. The outcome of these investigations will be the knowledge necessary to choose appropriate chemistries for sample matrix modification and analyte speciation control and chemistries for rapid and selective separation and preconcentration of target radionuclides from complex sample matrices. In addition, new approaches for quantification of alpha emitters in solution using solid state diode detectors, as well as improved instrumentation and signal processing techniques for use with solid-state and scintillation detectors, will be developed. New knowledge of the performance of separation materials, matrix modification and speciation control chemistries, instrument configurations, and quantitative analytical approaches will provide the basis for designing effective instrumentation for radioanalytical process monitoring. Specific analytical targets include 99 Tc, 90Sr and TRU actinides.
Methyl (3R)-3-hydroxyhex-5-enoate as a precursor to chiral mevinic acid analogues (1991)

Baker's yeast reduction of methyl 3-oxohex-5-enoate 14b provides methyl (3R)-3-hydroxyhex-5-enoate 15b with 78% enantiomeric enrichment. Subsequent seleno- and iodo-lactonization of derived hex-5-enoic acids leads to valerolactones 18, 19, 25 and 26 which are suitable for the subsequent elaboration of a variety of mevinic acid analogues. The absolute configuration of the major enantiomer produced in the initial yeast reduction was determined by correlation with natural (S)-(+)-parasorbic acid 23.
Alternatively activated macrophages engage in homotypic and heterotypic interactions through IL-4 and polyamine-induced E-cadherin/catenin complexes (2009)

Alternatively activated macrophages (AAMs), triggered by interleukin-4 (IL-4) and IL-13, play a modulating role during Th2 cytokine-driven pathologies, but their molecular armament remains poorly characterized. Here, we established E-cadherin (Cdh1) as a selective marker for IL-4/IL-13-exposed mouse and human macrophages, which is STAT6-dependently induced during polarized Th2 responses associated with Taenia crassiceps helminth infections or allergic airway inflammation. The IL-4-dependent, arginase-1/ornithine decarboxylase-mediated production of polyamines is important for maximal Cdh1 induction, unveiling a novel mechanism for IL-4-dependent gene transcription. At the macrophage surface, E-cadherin forms a functional complex with the catenins that accumulates at sites of cell contact. Macrophage-specific deletion of the Cdh1 gene illustrates the implication of E-cadherin in IL-4-driven macrophage fusion and heterotypic interactions with CD103(+) and KLRG1(+) T cells. This study identifies the E-cadherin/catenin complex as a discriminative, partly polyamine-regulated feature of IL-4/IL-13-exposed alternatively activated macrophages that contributes to homotypic and heterotypic cellular interactions.
Optimization of Fluorescence Labeling of Trace Analytes: Application to Amino Acid Biosignature Detection with Pacific Blue (2021)

Fluorescence labeling of biomolecules and fluorescence detection platforms provide a powerful approach to high-sensitivity bioanalysis. Reactive probes can be chosen to target specific functional groups to enable selective analysis of a chosen class of analytes. Particularly, when targeting trace levels of analytes, it is important to optimize the reaction chemistry to maximize the labeling efficiency and minimize the background. Here, we develop methods to optimize the labeling and detection of Pacific Blue (PB)-tagged amino acids. A model is developed to quantitate labeling kinetics and completeness in the circumstance where analyte labeling and reactive probe hydrolysis are in competition. The rates of PB hydrolysis and amino acid labeling are determined as a function of pH. Labeling kinetics and completeness as a function of PB concentration are found to depend on the ratio of the hydrolysis time to the initial labeling time, which depends on the initial PB concentration. Finally, the optimized labeling and detection conditions are used to perform capillary electrophoresis analysis demonstrating 100 pM sensitivities and high-efficiency separations of an 11 amino acid test set. This work provides a quantitative optimization model that is applicable to a variety of reactive probes and targets. Our approach is particularly useful for the analysis of trace amine and amino acid biosignatures in extraterrestrial samples. For illustration, our optimized conditions (reaction at 4 °C in a pH 8.5 buffer) are used to detect trace amino acid analytes at the 100 pM level in an Antarctic ice core sample.
Renal Replacement Therapy: Physical Properties of Hollow Fibers Influence Efficiency (2001)

In contrast to our expectations, the filter with the longer hollow fibers had a better performance, as it ran longer and had lower TMP This may be due to slower blood flow leading to an increase in blood viscosity in a filter with a larger cross section.
A Porous Polyaromatic Solid for Vapor Adsorption of Xylene with High Efficiency, Selectivity, and Reusability (2022)

Development of porous materials capable of capturing volatile organic compounds (VOCs), such as benzene and its derivatives, with high efficiency, selectivity, and reusability is highly demanded. Here we report unusual vapor adsorption behavior toward VOCs by a new porous solid, composed of a polyaromatic capsule bearing a spherical nanocavity with subnano-sized windows. Without prior crystallization and high-temperature vacuum drying, the porous polyaromatic solid exhibits the following five features: vapor adsorption of benzene over cyclohexane with 90 % selectivity, high affinity toward o-xylene over benzene and toluene with >80 % selectivity, ortho-selective adsorption ability (>50 %) from mixed xylene isomers, tight VOCs storage even under high temperature and vacuum conditions, and at least 5 times reusability for xylene adsorption. The observed adsorption abilities are accomplished at ambient temperature and pressure within 1 h, which has not been demonstrated by organic/inorganic porous materials reported previously.
P‐16.11: The Scheme Study and Halo Test of Vehicle Glass Based Mini LED Module (2022)

A 12.3‐inch Mini LED Module has been developed to realize highlight, high contrast and Local Dimming, Which is different from the ordinary side‐in backlight module, and the light‐emitting surface is directly below the module. The luminance is 1800nit and the contrast is more than 1,000,000:1. The module has passed the reliability of vehicle regulation. In the last the paper introduces the halo performance of the module.
MR Imaging at 3.0 T in Children: Technical Differences, Safety Issues, and Initial Experience (2009)

The high signal-to-noise ratio and contrast-to-noise ratio of 3.0-T magnetic resonance (MR) imaging can be used to obtain high-resolution thin-section images in a short acquisition time. These advantages are associated with an increased specific absorption rate (SAR) and more artifacts owing to B(1) inhomogeneity and increased susceptibility and chemical shift. Potential advantages of 3-T imaging in children include acquisition of good-quality images even with a small field of view (FOV). The shorter overall acquisition time of 3-T imaging is useful in children, who may not be able to cooperate for long. Shorter acquisition times also improve safety by reducing patient monitoring time within the enclosed bore of an MR imaging unit. SAR-related issues and dielectric artifacts are less problematic with a small FOV. Parallel imaging helps reduce SAR, susceptibility artifacts, and blurring of T2-weighted fast spin-echo (FSE) and single-shot FSE images by reducing the echo train length.
Imaging of the diffusion of single band 3 molecules on normal and mutant erythrocytes (2009)

Membrane-spanning proteins may interact with a variety of other integral and peripheral membrane proteins via a diversity of protein-protein interactions. Not surprisingly, defects or mutations in any one of these interacting components can impact the physical and biological properties on the entire complex. Here we use quantum dots to image the diffusion of individual band 3 molecules in the plasma membranes of intact human erythrocytes from healthy volunteers and patients with defects in one of their membrane components, leading to well-known red cell pathologies (hereditary spherocytosis, hereditary elliptocytosis, hereditary hydrocytosis, Southeast Asian ovalocytosis, and hereditary pyropoikilocytosis). After characterizing the motile properties of the major subpopulations of band 3 in intact normal erythrocytes, we demonstrate that the properties of these subpopulations of band 3 change significantly in diseased cells, as evidenced by changes in the microscopic and macroscopic diffusion coefficients of band 3 and in the compartment sizes in which the different band 3 populations can diffuse. Because the above membrane abnormalities largely arise from defects in other membrane components (eg, spectrin, ankyrin), these data suggest that single particle tracking of band 3 might constitute a useful tool for characterizing the general structural integrity of the human erythrocyte membrane.
Treatment of Hairy Cell Leukemia with Granulocyte Colony-Stimulating Factor and Recombinant Consensus Interferon or Recombinant Interferon-Alpha-2b (1992)

Patients with hairy cell leukemia and neutropenia (absolute neutrophil count less than 1.5 x 10(9)/L) were treated with recombinant granulocyte colony-stimulating factor (G-CSF) at doses of 3.6 and 7.2 micrograms/kg by daily subcutaneous injection, until normalization of neutrophil counts occurred. Patients then received either recombinant interferon-alpha-2b (r-IFN-alpha-2b) or a unique IFN, recombinant consensus IFN (rIFN-con-1), each given at doses of 10 micrograms/m2 subcutaneously three times a week, coupled with continued daily G-CSF therapy, for 3 months. After 3 months the G-CSF was discontinued; patients continued to take IFN for 1 year. All 10 patients responded to G-CSF with normalization of neutrophil counts within 2 weeks; the increase in neutrophil counts was greater in previously splenectomized patients. Four patients were treated with r-IFN-alpha-2b, and six were treated with rIFN-con-1. No patients developed recurrent neutropenia with the initiation of IFN therapy. Nine patients are evaluable for response to IFN. Five of six patients demonstrated hematologic improvement with rIFN-con-1, with two patients obtaining complete responses. All three patients receiving r-IFN-alpha-2b demonstrated hematologic improvement; one complete response was observed. Toxicities of both IFNs included influenza-like symptoms. We conclude that G-CSF can abrogate the myelosuppressive effects of IFN, and may be a useful adjunct to this therapy in neutropenic patients. We conclude that rIFN-con-1, the product of a synthetic gene, has activity in the treatment of hairy cell leukemia, and merits clinical investigation in other settings.