gebhart/patrepeval huggingface dataset:
Optimal arterial resistance for the maximal stroke work studied in isolated canine left ventricle. (1985)

In a previous analysis of ventricular arterial interaction (Sunagawa et al., 1983), we represented the left ventricle as an elastic chamber which periodically increases its volume elastance to a value equal to the slope of the linear end-systolic pressure-volume relationship. Similarly, the arterial load property was represented by an effective elastance which is the slope of the arterial end-systolic pressure-stroke volume relationship. Since the maximal transfer of potential energy from one elastic chamber to another occurs when they have equal elastance, we hypothesized that the left ventricle would do maximal external work if the ventricular elastance and the effective arterial elastance were equal. We tested this hypothesis in 10 isolated canine left ventricles, ejecting into a simulated arterial impedance, by extensively altering arterial resistance and finding the optimal resistance that maximized left ventricular stroke work under various combinations of end-diastolic volume, contractility, heart rate, and arterial compliance. Each of these parameters was set at one of three levels while others were at control. The optimal resistance varied only slightly with arterial compliance, whereas it varied widely with contractility and heart rate. We thus determined that the ratio of the optimal effective arterial elastance to the given ventricular elastance remained nearly unity. This result supports the hypothesis that the left ventricle does maximal external work to the arterial load when the ventricular and arterial elastances are equalized.
3P-075 Substrate d-camphor binding induces structural change of cytochrome P450cam(Heme proteins,The 47th Annual Meeting of the Biophysical Society of Japan) (2009)

TheBiophysicalSociety of JapanGeneral IncorporatedAssociation of the export process However the efficiency of finding the basal body with the FliHJFhJ Lomplex attaLhed is still lo-The seaiLh for optimum mix]ng ratio and
Red-shift experiments and non-Newtonian gravitational forces (1989)

The contribution of scalar non-Newtonian gravitational forces to the gravitational red-shifts is derived. The constraints imposed on scalar couplings by red-shift experiments are shown to be not competitive with those imposed by geophysical inverse-square-law experiments. 7 refs.
Faculty Opinions recommendation of Ambulatory blood pressure monitoring after 1 year on valsartan or amlodipine-based treatment: a VALUE substudy. (2007)

OBJECTIVE: The ambulatory blood pressure (ABP) monitoring substudy of the Valsartan Antihypertensive Long-term Use Evaluation (VALUE) trial was carried out in a subset of patients from USA, Italy and Denmark. ABP was measured after 1 year in the trial, with the aim of evaluating comparability of ABP levels on valsartan (VAL) and amlodipine (AML)-based regimens.METHODS: ABP was measured every 20 min during a 25-h period after morning administration of medicine; 659 patients were available for intention-to-treat analysis.RESULTS: Office blood pressure (BP) differences were smaller than in the main study and mean ABP levels also showed only minor differences between the two regimens (VAL, 132.5/74.8 mmHg; AML, 131.5/75.2 mmHg). However, during the first 7 h after dosing, ABP was lower on VAL, whereas AML exerted a significantly stronger effect during the last 4 h of the dosing interval--possibly influencing the differences in office BP found in the main study. Mean heart rate (HR) was higher on AML (72.3 bpm) than on VAL (70.5 bpm) (P = 0.013), suggesting a sustained difference in sympathetic activation. Correlation analysis showed a close relationship between treated ABP levels and the occurrence of combined cardiovascular endpoints--superior to the relationship to office BP.CONCLUSIONS: In these elderly high-risk patients, diastolic ABP levels tended to be less predictive than systolic, and daytime less predictive than night-time for all cardiovascular endpoints. The findings underline the importance of ABP substudies in comparative trials for elucidating significant differences in pharmacodynamics, and stresses the superior predictive power of ABP. PMID: 17278988
Activation of suppressor cells is genetically restricted by the I-J Subregion (1983)

Evidence describing T cell-mediated suppression of T cell reactivities (1–4), humoral responses (5), and even other suppressor T (Ts) cells (6,7) suggests an important regulatory role for these cells. Manipulation of the Ts pathway may also have important clinical applications. For instance, in murine systems, altering suppressor signals has been shown to have an effect on: growth of a tumor challenge (8), development of experimental arthritis (9), encephalitis (10), as well as acceptance of allografts (11).
The novel oxidoreductase KDRF (KM-102-derived reductase-like factor) is identical with human thioredoxin reductase (1997)

Correspondence| July 01 1997 The novel oxidoreductase KDRF (KM-102-derived reductase-like factor) is identical with human thioredoxin reductase Antonio MIRANDA-VIZUETE; Antonio MIRANDA-VIZUETE 1Department of Biosciences at Novum, Karolinska Institute, S-141 57 Huddinge, Sweden Search for other works by this author on: This Site PubMed Google Scholar Giannis SPYROU Giannis SPYROU * 1Department of Biosciences at Novum, Karolinska Institute, S-141 57 Huddinge, Sweden *To whom correspondence should be sent, at the following address: Department of Biosciences, Center for Biotechnology, Karolinska Institutet, Novum, S-141 57 Huddinge, Sweden. Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1997) 325 (1): 287–288. https://doi.org/10.1042/bj3250287 Article history Received: February 24 1997 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation Antonio MIRANDA-VIZUETE, Giannis SPYROU; The novel oxidoreductase KDRF (KM-102-derived reductase-like factor) is identical with human thioredoxin reductase. Biochem J 1 July 1997; 325 (1): 287–288. doi: https://doi.org/10.1042/bj3250287 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Journal Search Advanced Search The Biochemical Society, London © 19971997 Article PDF first page preview Close Modal You do not currently have access to this content.
Targeting proline in (phospho)proteomics (2019)

Mass spectrometry-based proteomics experiments typically start with the digestion of proteins using trypsin, chosen because of its high specificity, availability, and ease of use. It has become apparent that the sole use of trypsin may impose certain limits on our ability to grasp the full proteome, missing out particular sites of post-translational modifications, protein segments, or even subsets of proteins. To tackle this problem, alternative proteases have been introduced and shown to lead to an increase in the detectable (phospho)proteome. Here, we argue that there may be further room for improvement and explore the protease EndoPro. For optimal peptide identification rates, we explored multiple peptide fragmentation techniques (HCD, ETD, and EThcD) and employed Byonic as search algorithm. We obtain peptide IDs for about 40% of the MS2 spectra (66% for trypsin). EndoPro cleaves with high specificity at the C-terminal site of Pro and Ala residues and displays activity in a broad pH range, where we focused on its performance at pH = 2 and 5.5. The proteome coverage of EndoPro at these two pH values is rather distinct, and also complementary to the coverage obtained with trypsin. As about 40% of mammalian protein phosphorylations are proline-directed, we also explored the performance of EndoPro in phosphoproteomics. EndoPro extends the coverable phosphoproteome substantially, whereby both the, at pH = 2 and 5.5, acquired phosphoproteomes are complementary to each other and to the phosphoproteome obtained using trypsin. Hence, EndoPro is a powerful tool to exploit in (phospho)proteomics applications.
N-octanoyl Dopamine Improves Vascular Barrier Function Independent of Its Cytoprotective Properties in the Setting of Static Cold Preservation. (2014)

Objective: Static cold preservation (CP) severely impairs endothelial barrier function in solid organ allografts, due to endothelial cells (EC) necrosis, detachment and cell-body retraction. Although N-octanoyl dopamine (NOD) treatment can prevent the former two processes intercellular gap formation is still occurs upon CP. We analyzed the effect of NOD treatment of EC in relation to barrier function and assessed how NOD affects changes in the actin cytoskeleton and focal adhesion contacts. Methods: Impedance was measured in NOD treated or untreated HUVEC during treatment and after CP and re-warming. Changes in F-actin and Paxillin (pax) staining were studied by immune-fluorescence and Western blotting. Results: NOD strongly increased impedance when HUVEC were cultured at 37°C, paralleled by increased F-actin fibers, mostly associated with phosphorylated paxillin (pPax). This was completely abrogated in the presence of U0126 or Fasudil suggesting activation of p42/p44 and ROCK. Neither U0126 nor Fasudil impaired the propensity of NOD to protect EC against cold inflicted injury. While in untreated HUVEC subjected to CP impedance was strongly diminished, in NOD treated HUVEC impedance rapidly increased during re-warming. The expression of F-actin fibres and pPax increased in parallel to activation of p42/44. Phosphorylation of pax was strongly diminished in the presence of U0126.While inhibition of ROCK did not affect impedance; this was strongly abrogated in the presence of U0126. Conclusions: NOD treatment of EC increase impedance suggesting improved endothelial barrier properties. This is mediated through the activation of ROCK and p42/44 and involves activation of focal adhesion contacts. During re-warming gap closure does not depend on ROCK activation but requires p42/p44.NOD pre-conditioning of the donor organ may not only directly protect the EC of the allograft but may also improve vascular barrier properties at the time of reperfusion.
Rücktitelbild: Analysis of Powders Containing Illicit Drugs Using Magnetic Levitation (Angew. Chem. 2/2020) (2019)

Ein pulverförmiges Gemisch wurde in seine fünf Komponenten getrennt, und zwar in einer unpolaren paramagnetischen Lösung in einer 2.5 cm hohen Küvette in einem Magnetschwebegerät. Wie J. S. Bozenko, G. M. Whitesides et al. in ihrem Forschungsartikel auf S. 884 zeigen, trennt das Verfahren die Verbindungen basierend auf ihren unterschiedlichen Dichten in dem linearen Dichtegradienten, der in der Lösung gebildet wird. Die schwebenden Verbindungen (siehe Bild, aufgenommen mit gekreuzten Polarisatoren) sind gängige polare Verfälschungs- und Verdünnungsmittel, die zum Verschneiden illegaler Drogen verwendet werden.
Learning Task-Distribution Reward Shaping with Meta-Learning (2021)

Reward shaping is one of the most effective methods to tackle the crucial yet challenging problem of credit assignment and accelerate Reinforcement Learning. However, designing shaping functions usually requires rich expert knowledge and hand-engineering, and the difficulties are further exacerbated given multiple tasks to solve. In this paper, we consider reward shaping on a distribution of tasks that share state spaces but not necessarily action spaces. We provide insights into optimal reward shaping, and propose a novel meta-learning framework to automatically learn such reward shaping to apply on newly sampled tasks. Theoretical analysis and extensive experiments establish us as the state-of-the-art in learning task-distribution reward shaping, outperforming previous such works (Konidaris and Barto 2006; Snel and Whiteson 2014). We further show that our method outperforms learning intrinsic rewards (Yang et al. 2019; Zheng et al. 2020), outperforms Rainbow (Hessel et al. 2018) in complex pixel-based CoinRun games, and is also better than hand-designed reward shaping on grids. While the goal of this paper is to learn reward shaping rather than to propose new general meta-learning algorithms as PEARL (Rakelly et al. 2019) or MQL (Fakoor et al. 2020), our framework based on MAML (Finn, Abbeel, and Levine 2017) also outperforms PEARL / MQL, and could combine with them for further improvement.
A chimeric protein comprising the glucosyltransferase and cysteine proteinase domains of toxin B and the receptor binding domain of toxin A induces protective immunity againstClostridium difficileinfection in mice and hamsters (2015)

Clostridium difficile is the major cause of hospital-acquired infectious diarrhea and colitis in developed countries. The pathogenicity of C. difficile is mainly mediated by the release of 2 large potent exotoxins, toxin A (TcdA) and toxin B (TcdB), both of which require neutralization to prevent disease occurrence. We have generated a novel chimeric protein, designated mTcd138, comprised of the glucosyltransferase and cysteine proteinase domains of TcdB and the receptor binding domain of TcdA and expressed it in Bacillus megaterium. To ensure that mTcd138 is atoxic, 2 point mutations were introduced to the glucosyltransferase domain of TcdB, which essentially eliminates toxicity of mTcd138. Parenteral immunizations of mice and hamsters with mTcd138 induced protective antibodies to both toxins and provided protection against infection with the hyper-virulent C. difficile strain UK6.
Role of Molecular Sieves in the CVD Synthesis of Large‐Area 2D MoTe 2 (2016)

The synthesis of high-quality 2D MoTe2 with a desired phase on SiO2/Si substrate is crucial to its diverse applications. A side reaction of Te with the substrate Si leading to SiTe and Si2Te3 tends to happen during growth, resulting in the failure to obtain MoTe2. It has been found that molecular sieves can adsorb the silicon telluride byproducts and eliminate the influence of the side reaction during the chemical vapor deposition synthesis of MoTe2. With the help of molecular sieves, few-layer 1T′ MoTe2 can be grown from the MoOx precursor. Pure 1T′ MoTe2 and 2H MoTe2 regions in centimeter-sized areas synthesized on the same piece of SiO2/Si substrate can be obtained by using an overlapped geometry. The strategy provides a new method to controllably synthesize MoTe2 with desired phases and can be generalizable to the synthesis of other tellurium-based layered materials.
Identification of the Cell-Surface Protease ADAM9 as an Entry Factor for Encephalomyocarditis Virus (2019)

Encephalomyocarditis virus (EMCV) is an animal pathogen and an important model organism, whose receptor requirements are poorly understood. Here, we employed a genome-wide haploid genetic screen to identify novel EMCV host factors. In addition to the previously described picornavirus receptors sialic acid and glycosaminoglycans, this screen unveiled important new host factors for EMCV. These factors include components of the fibroblast growth factor (FGF) signaling pathway, such as the potential receptors FGFR1 and ADAM9, a cell-surface metalloproteinase. By employing various knockout cells, we confirmed the importance of the identified host factors for EMCV infection. The largest reduction in infection efficiency was observed in cells lacking ADAM9. Pharmacological inhibition of the metalloproteinase activity of ADAM9 did not affect virus infection. Moreover, reconstitution of inactive ADAM9 in knockout cells restored susceptibility to EMCV, pointing to a proteinase-independent role of ADAM9 in mediating EMCV infection. Using neutralization assays with ADAM9-specific antiserum and soluble receptor proteins, we provided evidence for a role of ADAM9 in EMCV entry. Finally, binding assays showed that ADAM9 facilitates attachment of EMCV to the cell surface. Together, our findings reveal a role for ADAM9 as a novel receptor or cofactor for EMCV.<b>IMPORTANCE</b> EMCV is an animal pathogen that causes acute viral infections, usually myocarditis or encephalitis. It is thought to circulate mainly among rodents, from which it is occasionally transmitted to other animal species, including humans. EMCV causes fatal outbreaks of myocarditis and encephalitis in pig farms and zoos, making it an important veterinary pathogen. Although EMCV has been widely used as a model to study mechanisms of viral disease in mice, little is known about its entry mechanism. Here, we employ a haploid genetic screen for EMCV host factors and identify an essential role for ADAM9 in EMCV entry.
Design Considerations for Energy Efficient DWDM PAM4 Transceivers Employing Avalanche Photodiodes (2020)

Abstract An effective approach to increase per‐fiber bandwidth is to combine per‐wavelength four‐level pulse amplitude modulation (PAM4) with dense wavelength division multiplexing (DWDM). Silicon photonic transceivers based on microring resonator modulators and drop filters can allow for an efficient realization of this. Utilizing avalanche photodiodes (APDs) in the receiver front‐ends of these transceivers can significantly improve sensitivity, allowing for reduced source laser power and overall improved power efficiency. This paper analyzes the optical‐modulation amplitude (OMA) sensitivity of these APD‐based PAM4 receivers in a silicon photonic DWDM link. A comprehensive link budget is considered that takes into account various system losses and relevant noise sources. Modeling with a developed APD that has a maximum 230 GHz gain‐bandwidth product predicts sensitivity improvements at 32 to 64 Gb s −1 PAM4 ranging from 9.8 to 12 dB relative to a conventional p‐i‐n photodiode. Scaling the APD gain‐bandwidth to 310 GHz allows support of 112 Gb s −1 with −13.2 dBm OMA sensitivity at a BER of with an APD gain of 10. Utilizing the presented architecture, link budget analysis predicts a 9.5x reduction in laser power with APDs and that 38 mW source laser optical power can support a four‐channel DWDM link operating at an aggregate 448 Gb s −1 data rate.
Abstract 9763: Brain-targeted Intranasal Administration of Viral Inhibition Peptide of Toll-like Receptor 4 Improves Survival and Prevents Left Ventricular Remodeling Via Sympathoinhibition in Ischemia-induced Heart Failure (2012)

[Backgrounds] Abnormal sympathoexcitation causes left ventricular (LV) remodeling and worsen the prognosis of ischemia-induced heart failure (HF). Recent studies have suggested that inflammation in the brain induces sympathoexcitation in HF. We previously demonstrated that brain inflammatory cascade mediated by Toll-like receptor 4 (TLR4) is activated in HF associated with sympathoexcitation. However, brain-specific inhibitory agent of TLR4 has not been available. The aim of the present study was to investigate whether intranasal administration of viral inhibition peptide of TLR4 (VIPER) could inhibit brain-specific TLR4 or not, and whether the brain-targeted inhibition of TLR4 could improve survival rate and prevent LV remodeling via sympathoinhibition in ischemia-induced HF or not. [Methods and Results] Sprague-Dawley rats with HF by coronary artery ligation were initiated the treatment with a dairy intranasal administration of VIPER (VIP), dairy intraperitoneal administration of VIPER (IP) or vehicle (Veh) just after the operation. At day 1, the expressions of TLR4, MyD88 (second messenger of TLR4), and nuclear factor κB (NFκB) in the brain were already significantly lower in VIP than in IP and Veh. These effects in VIP group were confirmed continuously in terms of the treatments. At day 7, urinary norepinephrine excretion (UNE; parameter of sympathoexcitation) was significantly lower and LV end-systolic dimension (LVESD) were significantly smaller in VIP than in Veh (uNE; 1.6±0.2 μg/day vs. 2.5±0.2 μg/day, LVESD; 2.9±0.3 mm vs. 4.8±0.2 mm, n=15 for each, p&lt;0.05 for each). At day 30, the survival rate was significantly higher in VIP than in Veh (84 % vs 61 %, P&lt;0.05). All of these beneficial results in VIP group were not obtained in IP group. [Conclusion] Intranasal administration of viral inhibition peptide of TLR4 is able to inhibit brain-specific TLR4, and improves survival rate and prevents LV remodeling via sympathoinhibition in ischemia-induced HF. This method has a potential to be a novel treatment for ischemia-induced HF with sympathoinhibition.
Möbius-invariant natural neighbor interpolation (2003)

We propose an interpolation method invariant under Mobius transformations: interpolation followed by transformation gives the same result as transformation followed by interpolation. The method uses natural (Delaunay) neighbors, but weights neighbors according to angles formed by Delaunay circles.
Highly Efficient Room Temperature Spin Injection in a Metal-Insulator-Semiconductor Light-Emitting Diode (2003)

We demonstrate highly efficient spin injection at low and room temperature in an AlGaAs/GaAs semiconductor heterostructure from a CoFe/AlOx tunnel spin injector. We use a double-step oxide deposition for the fabrication of a pinhole-free AlOx tunnel barrier. The measurements of the circular polarization of the electroluminescence in the Oblique Hanle Effect geometry reveal injected spin polarizations. of at least 24% at 80 K and 12% at room temperature.
Xanthine Oxidoreductase Inhibition Causes Reverse Remodeling in Rats With Dilated Cardiomyopathy (2005)

Increased reactive oxygen species (ROS) generation is implicated in cardiac remodeling in heart failure (HF). As xanthine oxidoreductase (XOR) is 1 of the major sources of ROS, we tested whether XOR inhibition could improve cardiac performance and induce reverse remodeling in a model of established HF, the spontaneously hypertensive/HF (SHHF) rat. We randomized Wistar Kyoto (WKY, controls, 18 to 21 months) and SHHF (19 to 21 months) rats to oxypurinol (1 mmol/L; n=4 and n=15, respectively) or placebo (n=3 and n=10, respectively) orally for 4 weeks. At baseline, SHHF rats had decreased fractional shortening (FS) (31±3% versus 67±3% in WKY, P &lt;0.0001) and increased left-ventricular (LV) end-diastolic dimension (9.7±0.2 mm versus 7.0±0.4 mm in WKY, P &lt;0.0001). Whereas placebo and oxypurinol did not change cardiac architecture in WKY, oxypurinol attenuated decreased FS and elevated LV end-diastolic dimension, LV end-systolic dimension, and LV mass in SHHF. Increased myocyte width in SHHF was reduced by oxypurinol. Additionally, fetal gene activation, altered calcium cycling proteins, and upregulated phospho–extracellular signal–regulated kinase were restored toward normal by oxypurinol ( P &lt;0.05 versus placebo-SHHF). Importantly, SHHF rats exhibited increased XOR mRNA expression and activity, and oxypurinol treatment reduced XOR activity and superoxide production toward normal, but not expression. On the other hand, NADPH oxidase activity remained unchanged, despite elevated subunit protein abundance in treated and untreated SHHF rats. Together these data demonstrate that chronic XOR inhibition restores cardiac structure and function and offsets alterations in fetal gene expression/Ca 2+ handling pathways, supporting the idea that inhibiting XOR-derived oxidative stress substantially improves the HF phenotype.
2′‐Deoxy‐2′‐C‐trifluoromethyl β‐D‐Ribonucleoside Analogues: Synthesis and Antiviral Evaluations (2004)

Abstract For Abstract see ChemInform Abstract in Full Text.