gebhart/patrepeval huggingface dataset:
Identification of emetine as a therapeutic agent that targets pigment epithelium derived factor - mediated inflammation and improves hyperglycemia in murine models of type 1 diabetes (IRM9P.728) (2014)

Abstract Pigment epithelium derived factor (PEDF) is a 50kDa secreted protein with inflammatory, antiangiogenic and neurotrophic properties. Elevated levels of PEDF are significantly associated with metabolic diseases including type 1 and type 2 diabetes, metabolic syndrome, and insulin resistance. PEDF administration to naïve animals result in the development of insulin resistance, and neutralization of PEDF with anti-PEDF antibodies improves the clinical outcome. Here we have identified a clinical drug candidate that attenuates PEDF mediated inflammation. Using a high-throughput screening approach, murine macrophages were stimulated with PEDF in the presence or absence of clinical drug candidates, and levels of secreted TNF analyzed. Following screening of 1,600 clinical candidate compounds, three lead compounds were identified, one being emetine, that dose dependently reduce PEDF induced macrophage activation without toxicity. Surface plasmon resonance analysis revealed that emetine binds to PEDF with high affinity (kD&amp;lt;25nM), and inhibits PEDF binding to its receptor adipose triglyceride lipase. Additionally, emetine reduces endocytosis of PEDF into macrophages. Administration of emetine to murine models of type 1 diabetes improves hyperglycemia, suppresses circulating levels of anti-insulin antibodies, and reduces pancreatic TNF and PEDF. Our studies suggest that emetine attenuates PEDF mediated inflammation, and has a therapeutic potential for treating type 1 diabetes.
The Influence of Three-Dimensional Computed Tomography Images of the Shoulder in Preoperative Planning for Total Shoulder Arthroplasty (2008)

The use of three-dimensional imaging can increase inter-rater agreement for the analysis of glenoid morphology and preoperative planning. Important considerations such as the extent and location of glenoid bone loss and the likelihood of implant fit were influenced by the three-dimensional data.
Three-dimensional morphology of nanoporous gold (2008)

We report transmission electron tomography of nanoporous gold fabricated by chemically dealloying Au35Ag65 films. A number of algorithms were employed to quantitatively characterize the complex three-dimensional nanoporous structure. It was found that gold ligaments and nanopore channels are topologically and morphologically equivalent, i.e., they are inverses of each other in three-dimensional space. Statistical analysis reveals that this bicontinuous nanostructured material is actually quasiperiodic and has, on average, a near zero surface curvature. These quantitative measurements will help in understanding the structural stability of nanoporous gold and in modeling its physical and chemical performances.
Stereospecific synthesis of cyclic adenosine 3',5'-(SP)-[18O]phosphate (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStereospecific synthesis of cyclic adenosine 3',5'-(SP)-[18O]phosphateJanina Baraniak, Krystyna Lesiak, Marek Sochacki, and Wojciech J. StecCite this: J. Am. Chem. Soc. 1980, 102, 13, 4533–4534Publication Date (Print):June 1, 1980Publication History Published online1 May 2002Published inissue 1 June 1980https://pubs.acs.org/doi/10.1021/ja00533a046https://doi.org/10.1021/ja00533a046research-articleACS PublicationsRequest reuse permissionsArticle Views72Altmetric-Citations26LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
N-terminal dual protein functionalization by strain-promoted alkyne–nitrone cycloaddition (2013)

Strain-promoted alkyne-nitrone cycloadditon (SPANC) was optimized as a versatile strategy for dual functionalization of peptides and proteins. The usefulness of the dual labeling protocol is first exemplified by the simultaneous introduction of a chloroquine and a stearyl moiety, two endosomal escape-improving functional groups, into the cell-penetrating peptide hLF (human lactoferrin). Additionally, we demonstrate that dual labeling of proteins is feasible by combining metal-free and copper-catalyzed click chemistry. First, SPANC is applied to enhanced green fluorescent protein to introduce both biotin and a terminal alkyne. The terminal acetylene then serves as a convenient anchor point for the CuAAC reaction with azido-containing fluorescein, thereby demonstrating the potential of combined SPANC and CuAAC for the straightforward, dual functionalization of proteins.
Adjuvants and the vaccine response to the DS-Cav1-stabilized fusion glycoprotein of respiratory syncytial virus (2017)

Appropriate adjuvant selection may be essential to optimize the potency and to tailor the immune response of subunit vaccines. To induce protective responses against respiratory syncytial virus (RSV)-a highly prevalent childhood pathogen without a licensed vaccine-we previously engineered a pre-fusion-stabilized trimeric RSV F (pre-F) "DS-Cav1" immunogen, which induced high titer RSV-neutralizing antibodies, in mice and non-human primates, when formulated with adjuvants Poly (I:C) and Poly (IC:LC), respectively. To assess the impact of different adjuvants, here we formulated RSV F DS-Cav1 with multiple adjuvants and assessed immune responses. Very high RSV-neutralizing antibody responses (19,006 EC50) were observed in naïve mice immunized with 2 doses of DS-Cav1 adjuvanted with Sigma adjuvant system (SAS), an oil-in-water adjuvant, plus Carbopol; high responses (3658-7108) were observed with DS-Cav1 adjuvanted with Alum, SAS alone, Adjuplex, Poly (I:C) and Poly (IC:LC); and moderate responses (1251-2129) were observed with DS-Cav1 adjuvanted with the TLR4 agonist MPLA, Alum plus MPLA or AddaVax. In contrast, DS-Cav1 without adjuvant induced low-level responses (6). A balanced IgG1 and IgG2a (Th2/Th1) immune response was elicited in most of the high to very high response groups (all but Alum and Adjuplex). We also tested the immune response induced by DS-Cav1 in elderly mice with pre-existing DS-Cav1 immunity; we observed that DS-Cav1 adjuvanted with SAS plus Carbopol boosted the response 2-3-fold, whereas DS-Cav1 adjuvanted with alum boosted the response 5-fold. Finally, we tested whether a mixture of ISA 71 VG and Carbopol would enhanced the antibody response in DS-Cav1 immunized calves. While pre-F-stabilized bovine RSV F induced very high titers in mice when adjuvanted with SAS plus Carbopol, the addition of Carbopol to ISA 71 VG did not enhance immune responses in calves. The vaccine response to pre-F-stabilized RSV F is augmented by adjuvant, but the degree of adjuvant-induced enhancement appears to be both context-dependent and species-specific.
Supplementary Video 4A and B from Selective Targeting and Potent Control of Tumor Growth Using an EphA2/CD3-Bispecific Single-Chain Antibody Construct (2023)

Supplementary Video 4A and B from Selective Targeting and Potent Control of Tumor Growth Using an EphA2/CD3-Bispecific Single-Chain Antibody Construct
Conversion of a Carboxylesterase into a Triacylglycerol Lipase by a Random Mutation (2005)

Klassenwechsel: Die Carboxylesterase R.34 (siehe Bild) kann in eine Triacylglycerinlipase überführt werden, ohne dass sich Größe, Form oder Hydrophobie des Substratbindungszentrums ändern. Die Substitution von Asn33 durch Asp führt zur Bildung einer Salzbrücke zwischen Asp33 und Arg49 und zur Verzerrung der Enzymstruktur, wodurch das katalytische Zentrum für größere Substrate besser zugänglich, aber auch labiler wird.
Nanoscale investigation of organic – inorganic halide perovskites (2015)

Over the last few years organic – inorganic halide perovskite-based solar cells have exhibited a rapid evolution, reaching certified power conversion efficiencies now surpassing 20%. Nevertheless the understanding of the optical and electronic properties of such systems on the nanoscale is still an open problem. In this work we investigate two model perovskite systems (based on iodine - CH3NH3PbI3 and bromine - CH3NH3PbBr3), analysing the local elemental composition and crystallinity and identifying chemical inhomogeneities.
A Dielectric Relaxometry Study of Segmental Dynamics in PDMS/Boron Composite and Hybrid Elastomers (2021)

Abstract not provided
125Mbit/s×3 Transmitter for Short-Distance Optical Link Using Low-Cost RGB-LED (2016)

A simple low-cost transmitter using low power RGB-type white LED is presented in this article. This type of white LED is widely used for illumination and communication applications. However, the low modulation bandwidth of RGB-LED, which is a few tens of MHz, limits the data rate of a transmission link. By applying a current-shaping-circuit (CSC), we can significantly improve the OOK-NRZ modulation speed of an AlGaInP red LED by a factor of 5. In this article, three color LEDs are separately modulated for data transmission. A data rate of 125Mbit/s×3 has been demonstrated with the aim to comply with Fast Ethernet (100Mbit/s) protocol.
Shape of the Capillary Meniscus around an Electrically Charged Particle at a Fluid Interface:  Comparison of Theory and Experiment (2006)

Here, we consider in detail the problem of the shape of the capillary meniscus around a charged colloidal particle, which is attached to a fluid interface: oil/water or air/water. The meniscus profile is influenced by the electric field created by charges at the particle/nonpolar fluid boundary. We digitized the coordinates of points from the meniscus around silanized glass spheres (200-300 mum in radius) attached to the tetradecane/water interface. The theoretical meniscus shape is computed in three different ways that give numerically coincident results. It is proven that for sufficiently small particles the meniscus profile can be expressed as a superposition of pure electric and gravitational deformations. Special attention is paid to the comparison of theory and experiment. A procedure for data processing is developed that allows one to obtain accurate values of the contact angle and surface charge density from the fit of the experimental meniscus profile. For all investigated particles, excellent agreement between theory and experiment is achieved. The results indicate that the electric field gives rise to an interfacial deformation of medium range and considerable amplitude.
Time-resolved small-angle x-ray scattering measurements of a polymer bicontinuous microemulsion structure factor under shear (2002)

In situ synchrotron x-ray scattering is used in conjunction with a novel annular cone and plate shear cell to study the nonequilibrium structure factor of a polymer bicontinuous microemulsion within the flow-gradient plane. At equilibrium the scattering is well described by the Teubner-Strey structure factor. In steady shear, the structure factor becomes highly anisotropic, owing to loss of scattering intensity along the flow direction and growth of intensity in peaks that progressively rotate towards the velocity-gradient direction. These results contrast with the predictions of a time-dependent Landau-Ginzburg model of Pätzold and Dawson, which generally predicts suppression of scattered intensity. The model assumption of a uniform velocity profile at the microemulsion length scale may be inappropriate owing to high viscosity contrast between the constituents of this sample. While the model anticipates a "stress-x-ray" rule, the data do not support its existence in this system. Nevertheless, strong connections do exist between x-ray anisotropy and stress during transient flow inception experiments. These connections break down upon flow cessation, where stress decays much more rapidly than anisotropy in the structure factor. The mechanical response of this sample exhibits a Rouse-like spectrum of relaxation times, whereas the second moment tensor used to characterize anisotropy in the structure factor exhibits nearly single-exponential relaxation. A phenomenological upper-convected-Maxwell/Lodge model for the second moment tensor provides essentially quantitative predictions of the structural response in step strain and oscillatory shear flow at moderate strains, although additional nonlinearity is found at higher strains.
Neoadjuvant dose-dense gemcitabine and cisplatin (DDGC) in patients (pts) with muscle-invasive bladder cancer (MIBC): Final results of a multicenter phase II study. (2014)

4513 Background: Neoadjuvant cisplatin (cis) based chemotherapy prior to radical cystectomy (RC) is standard of care for localized MIBC. Combination gemcitabine (gem) and cis is frequently used, but has not been tested prospectively. We sought to evaluate the activity of this combination when given in a dose dense fashion. Methods: Pts with MIBC, cT2-T4a, cN0-N1 with CrCl >=50 were eligible. Pts received 3 cycles of DDGC (gem 1200 mg/m2, cis 70mg/m2) on day 1, with pegfilgrastim 6 mg day 2 or 3, every 2 wks. Pts with CrCl 50-60 could receive cis split over 2 days. RC with lymph node dissection was to be performed 4-8 weeks after the last dose of DDGC. Primary endpoint was pathologic complete response (pT0) rate. Results: 32 pts were enrolled at 2 institutions (FCCC, TJU) over 14 months. One withdrew consent prior to receipt of DDGC. Among the 31 evaluable pts, median age was 69 (range 51 – 82). Baseline clinical stage was T2N0M0 (11), T3N0M0 (15), T4aN0M0/TxN1M0 (5). 25 pts received all 3 cycles of DDGC, with 1/25 requiring dose reduction of gem for thrombocytopenia. 6 discontinued DDGC early due to gr 3/4 vascular (3), gr 2/3 renal (2), or gr 3 hematologic (1) toxicity. Analysis of toxicities among the first 13 pts revealed 2 pts with MI (1 in cycle 1 (C1) with resulting CHF precluding surgery, 1 D#3 post-op) and 2 pts with VTE (1 PE in C2, 1 DVT D#15 post-op), and 1 pt who required CABG between DDGC and RC. The protocol was then amended to require cardiac clearance prior to study entry. Despite this, further vascular toxicity was seen with 1 DVT in C3 and 1 CVA in C1. The study was thus closed prior to target accrual of 44 pts. 30/31 pts underwent RC, 28 within 8 weeks of last DDGC. Median time from start of DDGC to RC was 9.3 weeks (range 5-29). 10/31 pts had pT0 (32%, 95% CI 16-48%). An additional 4 (13%) were downstaged to non-muscle invasive disease. Median f/up is 36 wks. 4 pts have recurred. 5 pts have died, 2 due to metastases. Conclusions: DDGC yielded pT0 rates similar to those reported with Accelerated MVAC. However significant vascular toxicity precluding, delaying or increasing the risk of surgery was noted, leading to early study closure. Clinical trial information: NCT01611662.
Acoustic Shielding of a Tapered Wing (2014)

Aircraft propulsion system noise can be a large contributor to ground level noise. Mounting the propulsor above the wing can reduce ground level noise below the vehicle. The level of acoustic shielding is dependent on wing planform dimensions and vehicle shape. Two different modeling approaches to predict noise shielding by tapered wings were investigated for use in an automated aircraft system modeling framework. The first approach uses the commercial code Virtual Lab (BEM, FEM, and Ray acoustics methods), and the second is the Maggi-Rubinowicz diffraction potential solution to the Kirchhoff Integral. Results from Virtual Lab were compared to two experimental results found in literature. Good agreement between Virtual Lab and experiment were obtained using the FEM. Acoustic shielding by tapered wings was calculated using FEM for the case of a single monopole noise source mounted on center just above the wing and compared to the Maggi-Rubinowicz diffraction potential method. Model development, analysis, and post processing were automated for both approaches so that they could be used as part of an aircraft system modeling framework. The FEM in Virtual Lab offers higher fidelity but higher computational cost, while the Maggi-Rubinowicz diffraction potential method is relatively lower fidelity since it is limited to use in diffraction problems with relatively simple geometry, and higher frequencies. A difference in shielding of several dB between the Maggi-Rubinowicz diffraction potential method compared with the FEM solution was noted, however, the diffraction potential method captures the correct trends in shielding and has much faster computational speed compared to FEM, which is beneficial for automated vehicle design routines.
Thermosensitive and Cell-Adhesive Pluronic Hydrogels for Human Adipose-Derived Stem Cells (2007)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.
Cryptographic Containers and the Digital Library (1997)

Today, information is distributed on the Internet and other communication infrastructures mainly for free. However, once information or digital contents is assigned some value, a means is needed to protect its copyrights and control its use. In this paper we describe an approach for rights management in the digital library. A digital library is a huge repository of digital content which is offered to the community of its users. The first step to control the use of its content is encryption. Encrypted content is not usable unless the decrypting or unlocking keys are acquired. The management of all keys involved in content transactions and their appropriate use is the task of rights management. After a short introduction into issues of electronic publishing we present in section 2 the cryptolopeTM 1 technology as a container for both encrypted content and information to purchase the content which serves as the basis for rights management. The second section will introduce the structure of cryptolopes and the procedures to use them. In the third section we will present the results of a digital library pilot project for rights management using cryptolope-like technology. The fourth section gives an outlook on advanced cryptolope transactions. We conclude the paper with a summary in section 5.
Development of a Method for Controlling Unstable Vehicle Behavior (2007)

&lt;div class="htmlview paragraph"&gt;It is important to estimate the vehicle state accurately in order to control vehicle behavior. In this study, we used the vehicle sideslip angle as a value indicating vehicle instability. A new method was developed that can estimate the sideslip angle accurately without being affected by driving conditions. This paper describes this new method and presents the results of evaluation tests that demonstrate its effectiveness.&lt;/div&gt;
Improved Air Spacer Co-Integrated with Self-Aligned Contact (SAC) and Contact over Active Gate (COAG) for Highly Scaled CMOS Technology (2020)

We report an improved air spacer that is successfully co-integrated on FinFET transistors with Self-Aligned Contacts (SAC) and Contacts Over Active Gate (COAG). The new integration scheme enables air spacer formation agnostic to the underlying transistor architecture, thus paving the way for a seamless adoption of air spacer in FinFET and Gate-All-Around (GAA) technologies. A reduction in effective capacitance (Ceff) by 15% is experimentally demonstrated. The power/performance benefits achieved by the new air spacer exceeds the benefits of scaling FinFET from 7nm node to 5nm node.
Electrochemical Polymerization of Aniline Investigated Using On-Line Electrochemistry/Electrospray Mass Spectrometry (1999)

A thin-layer electrochemical flow cell coupled on-line with electrospray mass spectrometry (EC/ES-MS) was used to investigate the soluble products from the controlled-potential anodic polymerization of aniline in H(2)O and H(2)O/CH(3)OH (1/1 v/v) with ammonium acetate and acetic acid or ammonium hydroxide as electrolytes (pH 4, 6.5, or 9). At a working electrode (glassy carbon) potential of 1.0 V versus Ag/AgCl, singly protonated aniline oligomers containing as many as 10 aniline units (10-mer) were observed in the ES mass spectra when the polymerization in H(2)O/CH(3)OH at pH 4 was carried out. The abundance of the higher n-mers decreased at higher solution pH and in 100% H(2)O at pH 4. Most of the oligomers were observed in more than one redox state ranging from fully oxidized (all imine nitrogens) to fully reduced (all amine nitrogens). The number of different redox states observed for the n-mers increased with increasing n. The structures of the reduced (m/z 185) and oxidized (m/z 183) aniline dimer ions (head-to-tail, tail-to-tail, or head-to-head) produced from the polymerization of aniline at pH 4, 6.5, and 9 in H(2)O/CH(3)OH were revealed to vary as a function of pH by comparison of their tandem mass spectrometry product ion spectra with the product ion spectra of the dimer standards. EC/ES-MS potential scan experiments, in which the working electrode current and major n-mer ions for n = 2, 3, and 4 were monitored as a function of electrode potential, were used to probe the growth mechanism to higher aniline oligomers. Under the conditions used, the controlled-current electrolytic process inherent to the operation of the ES ion source did not significantly influence the formation or nature of the oligomers observed. Beyond the current application, the results presented here serve to demonstrate the utility of EC/ES-MS as a tool in identifying the initial products of electropolymerization and in studying the products of electrode reactions in general.
VLSI Architectures for JPEG2000 (2004)

In this chapter, we have presented a VLSI architecture for the JPEG2000 encoder. We also presented the underlying details of the EBCOT architecture including its key building blocks and control mechanism. We presented the MQ-coder architecture for binary arithmetic coding. We also briefly presented the JPEG2000 decoder architecture which is architecturally similar with the encoder architecture with the data flow just in reverse direction with minor changes in the control mechanism and registers. There are not many architectures for the JPEG2000 algorithm available in the literature. Since entropy coding of the JPEG2000 is the most computationally intensive, few researchers focused on optimizing the EBCOT architecture in order to reduce clock cycle and memory requirements. We have reviewed these architectures in this chapter as well. Detail review of the VLSI architectures for lifting-based Discrete Wavelet Transform suitable for JPEG2000 has been presented in Chapter 5.