gebhart/patrepeval huggingface dataset:
Axicabtagene Ciloleucel (axi-cel; KTE-C19) in Patients with Refractory Aggressive Non-Hodgkin Lymphoma (NHL): Long-Term Follow-up of the Pivotal Zuma-1 Trial (2018)

Background: The SCHOLAR-1 pooled analysis demonstrated poor outcomes with current therapies in relapsed, aggressive NHL (26% objective response rate [ORR] and 7% complete response [CR]), with a median overall survival (OS) of 6.3 mo (Crump, Blood. In press). In the primary analysis of ZUMA-1, axi-cel achieved an ORR of 82% (54% CR) with a manageable safety profile. We present the 1-year follow-up (f/u) of ZUMA-1 to confirm the durability of response and report exploratory biomarker analyses to understand resistance mechanisms to anti-CD19 CAR T (axi-cel) therapy. Methods: Patients (pts) with refractory difuse large B cell lymphoma, transformed follicular lymphoma, or primary mediastinal large B cell lymphoma with prior anti-CD20 therapy and an anthracycline-containing regimen were enrolled and dosed per Locke et al. (AACR 2017). Refractory disease was defined as progressive or stable disease as best response to last therapy, or relapse ≤12 mo after autologous stem cell transplant. The primary endpoint was ORR (2007 IWG criteria). Key secondary endpoints included duration of response (DOR), OS, and incidence of adverse events (AEs). Results: Long-term analysis is being performed (data-cut August 11, 2017). To date, no pt has been lost to f/u, and all pts who are alive remain in f/u. Updated DOR and OS will be presented (minimum f/u: 1 y; f/u: 15 mo). Updated subgroup and associated efficacy analyses will be presented. Baseline and post-progression biopsies were evaluable by central review from 12 pts. CD19 and PD-L1 immunohistochemistry results are tabulated. Three of 11 (27%) pts with CD19+ status at baseline developed CD19– disease at progression. Eight of 10 (80%) pts evaluable for PD-L1 at progression had PD-L1+ disease. Of the 8 pts with CD19+ samples at progression, 5 (63%) demonstrated PD-L1+ tumor cells. Of the 3 pts with CD19– samples at progression, 2 had PD-L1+ tumor cells. In addition, post-progression biopsies from 6 separate pts were evaluable by local review, of which 3 (50%) had ≤ 1% CD19 staining. Cumulatively, 17 pts were evaluable for CD19 expression at time of progression by either central or local review, and 6 (35%) had ≤ 1% CD19 expression. Conclusions: In ZUMA-1, axi-cel demonstrated significant clinical benefit with manageable AEs in pts with no curative treatment options. Additional long-term data will be presented. Loss of CD19 and gain of PD-L1 tumor expression are identified as possible mechanisms of resistance following axi-cel treatment. These results provide insights into developing novel therapeutic strategies to overcome CD19 CAR T resistance and further improve outcomes in these pts. Drs. Neelapu and Locke contributed equally to this work.Table 1CD19 and PD-L1 Expression Status by Central ReviewCD19 StatusPD-L1 StatusAt BaselineAt ProgressionAt ProgressionEvaluable, n121210Positive, n1187Negative, n143 Open table in a new tab
An Integrated Analysis of Metabolites, Peptides, and Inflammation Biomarkers for Assessment of Preanalytical Variability of Human Plasma (2019)

Discrepancies in blood sample collection and processing could have a significant impact on levels of metabolites, peptides, and protein biomarkers of inflammation in the blood; thus, sample quality control is critical for successful biomarker identification and validation. In this study, we analyzed the effects of several preanalytical processing conditions, including different storage times and temperatures for blood or plasma samples and different centrifugation forces on the levels of metabolites, peptides, and inflammation biomarkers in human plasma samples using ethylenediaminetetraacetic acid (EDTA) as an anticoagulant. Temperature was found to be the major factor for metabolite variation, and both time and temperature were identified as major factors for peptide variation. For inflammation biomarkers, temperature played different roles depending on the sample type (blood or plasma). Low temperature affected inflammation biomarkers in blood, while room temperature impacted inflammation biomarkers in plasma.
ChemInform Abstract: Expedient Method for the Solid‐Phase Synthesis of Aspartic Acid Protease Inhibitors Directed Toward the Generation of Libraries. (1995)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
In Vitro Models for Studying Renal Stone Formation: A Clear Alternative (1998)

This paper discusses the limitations of using laboratory animals for direct in vivo observation of the development of renal stones. In fact, the majority of hypotheses related to mechanisms of stone formation have been based on the results of in vitro experiments. The relevance of in vitro experiments that allow the study of urolithiasis depends upon the degree of correspondence between the experimental conditions and those prevailing in the stone-forming kidney in vivo. For this reason, several in vitro experimental systems that attempt to reproduce the conditions found in vivo have been developed in order to study renal stone formation, which have been classified into two main groups: a) models to study papillary stone formation; and b) models to study "sedimentary" stone formation. These models are briefly described in this paper, and the information obtained was compared with that resulting from a study of the fine structure of real human renal calculi, in order to prove the validity of the models. It was concluded that the experimental in vitro models can closely reproduce the renal conditions under which human calculi are developed. This allows important data to be obtained about the aetiology of renal lithiasis, which is of great relevance to the development of effective treatments for this disease. Therefore, experimental in vitro models constitute a clear alternative to the use of laboratory animals.
Thermally-Sensitive and Magnetic Poly(<I>N</I>-Vinylcaprolactam)-Based Nanogels by Inverse Miniemulsion Polymerization (2012)

International audience
Learning to Teach through Recursive Boundary Crossing in the Teaching Practicum (2022)

Informed by a conceptual framework on boundary crossing, this qualitative case study explored how a student teacher engaged in professional learning through recursive boundary crossing between her field school and the university programme in a U.S. context. The findings revealed the power of boundary crossing as a cyclical, intense, and transformative learning mechanism that helped the student teacher connect teaching knowledge and experiences acquired at different sites and facilitated her reflective practice and self-transformation. However, the lack of communication between the university and the field school caused a cognitive disturbance and emotional challenges for the student teacher. The study concludes with practical implications for reforming teaching practicum to maximise the potential of boundary crossing for teacher learning.
Monte Carlo simulations of the hydration of ammonium and carboxylate ions (1986)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMonte Carlo simulations of the hydration of ammonium and carboxylate ionsWilliam L. Jorgensen and Jiali GaoCite this: J. Phys. Chem. 1986, 90, 10, 2174–2182Publication Date (Print):May 1, 1986Publication History Published online1 May 2002Published inissue 1 May 1986https://pubs.acs.org/doi/10.1021/j100401a037https://doi.org/10.1021/j100401a037research-articleACS PublicationsRequest reuse permissionsArticle Views1620Altmetric-Citations277LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Growth factor responsiveness of human retinal pigment epithelial cells. (1990)

Growth factor effects on DNA synthesis in density-arrested human retinal pigment epithelial cells were assessed by [3H]-thymidine incorporation. Acidic and basic fibroblast growth factor and epidermal growth factor were potent stimulators, whereas platelet-derived growth factor, insulinlike growth factor-1, and insulin were weak or modest stimulators when used alone. When used in combination, each of the above growth factors caused a significant enhancement of [3H]-thymidine incorporation regardless of its effect when used alone. The combination of all four growth factors was significantly more effective than all other combinations, demonstrating synergism in their action. Similar results were found in cell proliferation assays. In contrast to this, transforming growth factor-beta inhibited the ability of each of the other growth factors and serum-containing media to stimulate [3H]-thymidine incorporation. These data suggest that DNA synthesis in human retinal pigment epithelial cells can be modulated by several growth factors, some in a stimulatory or synergistic manner and at least one in an inhibitory manner. A better understanding of these complex interactions may provide insights relevant to normal and abnormal ocular wound healing.
Impaired Olfaction and Risk of Delirium or Cognitive Decline After Cardiac Surgery (2015)

Impaired olfaction is prevalent in individuals undergoing cardiac surgery and is associated with greater adjusted risk of postoperative delirium but not cognitive decline. Impaired olfaction may identify unrecognized vulnerability to postoperative delirium in individuals undergoing cardiac surgery.
On the Robustness of the Predistortion Function Synthesis for Highly Nonlinear RF Power Amplifiers Linearization (2006)

This paper investigates the robustness of the digital predistortion function synthesis process applied to linearize a highly non linear RF power amplifier. For this purpose, a 100-watts peak power amplifier designed for UMTS applications biased in deep class AB is used. Two approaches have been compared: the polynomial curve fitting of the measured AM/AM and AM/PM characteristics and the look-up tables synthesis using the smoothed versions of these same non linear curves. The polynomial based fitting presents badly conditioned matrices that make this approach unpractical for the considered non linearity shape particularly for DSP implementation of the synthesis procedure. The accuracy of both approaches was assessed by measuring the adjacent channel power ratio at the output of the power amplifier under a 2-carriers W-CDMA signal. The look-up tables approach based on the averaging technique showed better correction capabilities than the polynomial based fitting
Production of Copolymers in a Tubular Reactors Through Nitroxide Mediated Controlled Free‐Radical Polymerization (2013)

Summary This work presents experimental results for styrene / methyl methacrylate (MMA) copolymerizations performed in a tubular reactor equipped with lateral feed lines through the nitroxide mediated controlled free‐radical mechanism. Reactions were carried out in solution with a primary feed containing a mixture of styrene, toluene, initiators and TEMPO (2,2,6,6‐tetramethyl‐1‐piperidinoxyl) and a lateral feed containing a mixture of comonomers and TEMPO. The obtained results show that copolymers can be obtained with weight average molecular weights ranging from 30 × 10 3 to 60 × 10 3 g/gmol and polydispersities ranging from 1.3 to 1.6, with monomer conversions ranging from 20 to 70 wt%. When the average residence time is manipulated, it can be observed that the average molecular weights increase linearly with time, confirming the “living” character of the reaction system.
Synthesis and Electrochemical Characterization of a Transition-Metal-Modified Ligand−Receptor Pair (2005)

The energetics of weak interactions (van der Waals forces, hydrogen bonding) are difficult to quantify in biological ligand-receptor pairs. Insight into the biochemical role these forces play is critical to an understanding of signal transduction events and the drug discovery process. Ruthenium pentaammine and iron tetracyano complexes modified with either biotin or desthiobiotin have been synthesized and characterized. These modified biological ligands bind to the protein avidin in a manner similar to that of native biotin. Experiments using redox mediators show that the avidin-bound complexes are electrochemically accessible.
Catalytic Activation of Bioorthogonal Chemistry with Light (CABL) Enables Rapid, Spatiotemporally-controlled Labeling and No-Wash, Subcellular 3D-Patterning in Live Cells using Long Wavelength Light (2021)

Described is the spatiotemporally controlled labeling and patterning of biomolecules in live cells through the catalytic activation of bioorthogonal chemistry with light, referred to as “CABL”. Here, an unreactive dihydrotetrazine (DHTz) is photocatalytically oxidized in the intracellular environment by ambient O2 to produce a tetrazine that immediately reacts with a trans-cyclooctene (TCO) dieno-phile. 6-(2-Pyridyl)-dihydrotetrazine-3-carboxamides were developed as stable, cell permeable DHTz reagents that upon oxidation pro-duce the most reactive tetrazines ever used in live cells with Diels-Alder kinetics exceeding k2 106 M-1s-1. CABL photocatalysts are based on fluorescein or silarhodamine dyes with activation at 470 or 660 nm. Strategies for limiting extracellular production of singlet oxygen are described that increase the cytocompatibility of photocatalysis. The HaloTag self-labeling platform was used to introduce DHTz tags to proteins localized in the nucleus, mitochondria, actin or cytoplasm, and high-yielding subcellular activation and labeling with a TCO-fluorophore was demonstrated. CABL is light-dose dependent, and 2-photon excitation promotes CABL at the sub-organelle level to selectively pattern live cells under no-wash conditions. CABL was also applied to spatially resolved live-cell labeling of an endogenous pro-tein target by using TIRF microscopy to selectively activate intracellular monoacylglycerol lipase tagged with DHTz-labeled small mole-cule covalent inhibitor. Beyond spatiotemporally controlled labeling, CABL also improves the efficiency of ‘ordinary’ tetrazine ligations by rescuing the reactivity of commonly used 3-aryl-6-methyltetrazine reporters that become partially reduced to DHTzs inside cells. The spatiotemporal control and fast rates of photoactivation and labeling of CABL should enable a range of biomolecular labeling applications in living systems.
Enzyme stabilization by covalent binding in nanoporous sol‐gel glass for nonaqueous biocatalysis (2001)

A unique nanoporous sol-gel glass possessing a highly ordered porous structure (with a pore size of 153 A in diameter) was examined for use as a support material for enzyme immobilization. A model enzyme, alpha-chymotrypsin, was efficiently bound onto the glass via a bifunctional ligand, trimethoxysilylpropanal, with an active enzyme loading of 0.54 wt%. The glass-bound chymotrypsin exhibited greatly enhanced stability both in aqueous solution and organic solvents. The half-life of the glass-bound alpha-chymotrypsin was >1000-fold higher than that of the native enzyme, as measured either in aqueous buffer or anhydrous methanol. The enhanced stability in methanol, which excludes the possibility of enzyme autolysis, particularly reflected that the covalent binding provides effective protection against enzyme inactivation caused by structural denaturation. In addition, the activity of the immobilized alpha-chymotrypsin was also much higher than that of the native enzyme in various organic solvents. From these results, it appears that the glass-enzyme complex developed in the present work can be used as a high-performance biocatalyst for various chemical processing applications, particularly in organic media. Published by John Wiley & Sons
A novel approach for detecting viable and tissue‐specific circulating tumor cells through an adenovirus‐based reporter vector (2014)

Conditionally replicative adenoviral vectors and secreted reporters offer a functional method to detect viable CTCs with cell specificity and high sensitivity.
Collagen Mimetic Peptides as Probes for Bacterial Infection (2017)

Collagen is the most abundant protein in humans and comprises a major structural component of the extracellular matrix (ECM). In wounds, collagen triple helices suffer damage. As collagen prefers to form a triple helix, we can take advantage of the damaged collagen and introduce collagen mimetic peptides (CMPs) that will adhere to the wound surface. These invasive CMPs can be used to deliver a payload to the collagen surface of a wound. Nature may also use this invasive strand mechanism as a form of host infection. Invasion of host tissue can be mediated by the interaction of bacterial surface proteins and the ECM. Group A Streptococcus (GAS) displays cell‐surface proteins that resemble human collagen. We hypothesize that the collagen‐like strands of GAS form triple helices with denatured collagen in a wound bed. The ensuing adherence could be a basis for infection by GAS. To test this hypothesis, we are using fluorophore‐labeled CMPs to detect collagen strands on the surface of live GAS cells. We are also evaluating knock‐in expression of collagen‐like strands, Scl 1 and Scl 2, on the surface of B. subtilis to transfer the ability to anneal to our CMPs. With these tools we hope to understand and ultimately antagonize infections by GAS. Support or Funding Information This work is supported by Grant R01 AR044276 (NIH).
Plasma transport in the auroral return current region (1987)

The classical and anomalous transport properties of a multifluid plasma consisting of H + , O + , and electron populations in the presence of auroral field‐aligned return currents are investigated, using a multimoment fluid model with anomalous transport coefficients. This approach offers the possibility of simulating large‐scale dynamic phenomena without neglecting the important macroscopic consequences of microscopic processes such as anomalous resistivity and turbulent heating. The macroscopic effects of the electrostatic ion cyclotron (EIC) instability (perpendicular ion heating) and of an EIC‐related anomalous resistivity mechanism which heats the electrons are included in the present version of the model. The responses of the outflowing polar wind plasma to the application of current, with and without instabilities, are exhibited. The simulations show that the electron drift velocity corresponding to a return current of 0.65 µA/m² is above the threshold for EIC waves. Downward electron heat flow competes with upward convection and adiabatic effects to determine the direction of the electron temperature anisotropy. Resistive electron heating lowers the critical drift velocity for marginal EIC stability and leads to enhanced ion heating. The model predictions are compared with the experimental observations where possible.
Characterization for High-Performance CMOS Using In-Wafer Advanced Kelvin-Contact Device Structure (2009)

In this work, a new electrical characterization method for MOSFETs using an in-wafer Kelvin-contact device structure is developed. The developed method can eliminate the parasitic series resistance such as resistance in source/drain terminals of MOSFETs, in metal wires on wafers and in a measurement system. Using the developed method, we can measure and analyze the short channel transistors' intrinsic current-voltage characteristics as well as the quantitative effects of the parasitic series resistance to the device performance, very stably and accurately. In addition, a framework for the characterization of inversion layer mobility in ultrathin gate insulator MOSFETs with large gate current is provided. Based on the framework, the developed method is introduced as a suitable mobility characterization method.
ChemInform Abstract: Synthesis of D‐Glucose 3‐ and 6‐(2‐(Perfluoroalkyl)ethyl Phosphates): A New Type of Anionic Surfactant for Biomedical Use. (1992)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
An In Situ Cross‐Linked Nonaqueous Polymer Electrolyte for Zinc‐Metal Polymer Batteries and Hybrid Supercapacitors (2020)

This work reports the facile synthesis of nonaqueous zinc-ion conducting polymer electrolyte (ZIP) membranes using an ultraviolet (UV)-light-induced photopolymerization technique, with room temperature (RT) ionic conductivity values in the order of 10<sup>-3</sup> S cm<sup>-1</sup> . The ZIP membranes demonstrate excellent physicochemical and electrochemical properties, including an electrochemical stability window of >2.4 V versus Zn|Zn<sup>2+</sup> and dendrite-free plating/stripping processes in symmetric Zn||Zn cells. Besides, a UV-polymerization-assisted in situ process is developed to produce ZIP (abbreviated i-ZIP), which is adopted for the first time to fabricate a nonaqueous zinc-metal polymer battery (ZMPB; VOPO<sub>4</sub> |i-ZIP|Zn) and zinc-metal hybrid polymer supercapacitor (ZMPS; activated carbon|i-ZIP|Zn) cells. The VOPO<sub>4</sub> cathode employed in ZMPB possesses a layered morphology, exhibiting a high average operating voltage of ≈1.2 V. As compared to the conventional polymer cell assembling approach using the ex situ process, the in situ process is simple and it enhances the overall electrochemical performance, which enables the widespread intrusion of ZMPBs and ZMPSs into the application domain. Indeed, considering the promising aspects of the proposed ZIP and its easy processability, this work opens up a new direction for the emergence of the zinc-based energy storage technologies.
Electrostatic Self-Assembly of Polydiacetylene Nanocrystals:  Nonlinear Optical Properties and Chain Orientation (1999)

Two different sizes of nanocrystals of poly(1,6-dicarbazolyl-2,4-hexadiyne) (poly-DCHD), have been assembled into composite films by electrostatic self-assembly (ESA) using poly(dimethyldiallylammonium chloride) (PDAC) as the oppositely charged polyelectrolyte. UV−visible absorption showed that the film deposition process is uniform. The AFM images of these films indicate that the nanocrystals are platelets lying on the substrate plane. The chromophore (polydiacetylene backbone) orientation and third-order optical nonlinearity of poly-DCHD/PDAC multilayers are investigated by electroabsorption spectroscopy. The ratio of to is significantly less than 3, indicating that the polymer chains are predominantly parallel to the plane of the substrate. In addition, the films possess third-order nonlinearity in the order of 10-11 esu.