gebhart/patrepeval huggingface dataset:
Heat Shock Protein 60 Acts as a Receptor for theListeriaAdhesion Protein in Caco-2 Cells (2004)

The 104-kDa Listeria adhesion protein (LAP) in Listeria monocytogenes is involved in binding to various mammalian cell lines. However, the receptor that interacts with LAP in eukaryotic cells is unknown. In this study, scanning immunoelectron microscopy qualitatively demonstrated greater binding capacity of wild-type (WT) L. monocytogenes strain (F4244) than a LAP-deficient mutant strain (KB208) to Caco-2 cells. The goal of this study was identification of the host cell receptor for LAP. Using a Western blot ligand overlay assay, we identified a protein of 58 kDa to be the putative receptor for LAP from Caco-2 cells. N-terminal sequencing and subsequent database search identified this protein as heat shock protein 60 (Hsp60). Modified immunoseparation with protein A-Sepharose beads bound to the LAP-specific monoclonal antibody H7 (MAb-H7) and a sequential incubation with LAP preparation and Caco-2 lysate confirmed the receptor to be the same 58-kDa protein. Western blot analysis with anti-Hsp60 MAb of whole-cell adhesion between Caco-2 and WT also revealed the receptor protein to be a 58-kDa protein, thus corroborating the identification of Hsp60 as a host cell receptor for LAP. Furthermore, the anti-Hsp60 antibody also caused approximately 74% reduction in binding of L. monocytogenes WT to Caco-2 cells, whereas a control antibody, C11E9, had no effect on binding. The adhesion mechanism of L. monocytogenes to eukaryotic cells is a complex process, and identification of Hsp60 as a receptor for LAP adds to the list of previously discovered ligand-receptor modules that are essential to achieve successful adhesion.
Substratum-induced differentiation of human pluripotent stem cells reveals the coactivator YAP is a potent regulator of neuronal specification (2014)

Physical stimuli can act in either a synergistic or antagonistic manner to regulate cell fate decisions, but it is less clear whether insoluble signals alone can direct human pluripotent stem (hPS) cell differentiation into specialized cell types. We previously reported that stiff materials promote nuclear localization of the Yes-associated protein (YAP) transcriptional coactivator and support long-term self-renewal of hPS cells. Here, we show that even in the presence of soluble pluripotency factors, compliant substrata inhibit the nuclear localization of YAP and promote highly efficient differentiation of hPS cells into postmitotic neurons. In the absence of neurogenic factors, the effective substrata produce neurons rapidly (2 wk) and more efficiently (>75%) than conventional differentiation methods. The neurons derived from substrate induction express mature markers and possess action potentials. The hPS differentiation observed on compliant surfaces could be recapitulated on stiff surfaces by adding small-molecule inhibitors of F-actin polymerization or by depleting YAP. These studies reveal that the matrix alone can mediate differentiation of hPS cells into a mature cell type, independent of soluble inductive factors. That mechanical cues can override soluble signals suggests that their contributions to early tissue development and lineage commitment are profound.
A Time-of-Flight Image Sensor Using 8-Tap P-N Junction Demodulator Pixels (2023)

This paper presents a time-of-flight image sensor based on 8-Tap P-N junction demodulator (PND) pixels, which is designed for hybrid-type short-pulse (SP)-based ToF measurements under strong ambient light. The 8-tap demodulator implemented with multiple p-n junctions used for modulating the electric potential to transfer photoelectrons to eight charge-sensing nodes and charge drains has an advantage of high-speed demodulation in large photosensitive areas. The ToF image sensor implemented using 0.11 µm CIS technology, consisting of an 120 (H) × 60 (V) image array of the 8-tap PND pixels, successfully works with eight consecutive time-gating windows with the gating width of 10 ns and demonstrates for the first time that long-range (>10 m) ToF measurements under high ambient light are realized using single-frame signals only, which is essential for motion-artifact-free ToF measurements. This paper also presents an improved depth-adaptive time-gating-number assignment (DATA) technique for extending the depth range while having ambient-light canceling capability and a nonlinearity error correction technique. By applying these techniques to the implemented image sensor chip, hybrid-type single-frame ToF measurements with depth precision of maximally 16.4 cm (1.4% of the maximum range) and the maximum non-linearity error of 0.6% for the full-scale depth range of 1.0-11.5 m and operations under direct-sunlight-level ambient light (80 klux) have been realized. The depth linearity achieved in this work is 2.5 times better than that of the state-of-the-art 4-tap hybrid-type ToF image sensor.
Digital Coherent W-band MIMO Radio-over-Fiber System (2015)

W-band RoF transmission system with converged MIMO DSP for compensating signal impairments both in optical fiber and free-space is presented, particularly focusing on MDM transmission in a low differential mode group delay two-mode fiber link.
Ultrafast imaging for uncovering laser–material interaction dynamics (2022)

Abstract The physical mechanism of the dynamics in laser–material interaction has been an important research area. In addition to theoretical analysis, direct imaging‐based observation of ultrafast dynamic processes is an important approach to understand many fundamental issues in laser–material interaction such as inertial confinement fusion (ICF), laser accelerator construction, and advanced laser production. In this review, the principles and applications of three types of commonly used ultrafast imaging methods are introduced, including the pump–probe, X‐ray diagnosis, and single‐shot optical burst imaging. We focus on the technical features such as the spatial and temporal resolution for each technique, and present several conventional applications.
Response and Remission Are Associated with Improved Quality of Life in Patients with Ulcerative Colitis (2005)

Purpose: Data from ACT 1 & 2 (randomized, controlled trials of infliximab) were used to examine the relationship between health-related quality of life (HRQL) and response and remission. Methods: Based on wk 30 actual Mayo scores, 728 pts were categorized as non-responders, responders (those in response but not in remission), or remission. Response was defined as a decrease from baseline in Mayo score by ≥30% and ≥ 3 points, with a decrease in rectal bleeding subscore of ≥1 or rectal bleeding subscore of 0 or 1. Remission was defined as a Mayo score ≤2 points, with no individual subscore >1. HRQL was assessed using the IBDQ and the SF-36. Analysis of variance was used to compare IBDQ and SF-36 changes from baseline to wk 30 among groups. Results: At baseline the physical and mental component summary scores (PCS = 38 and MCS = 40) of the SF-36 were ∼ 1 standard deviation below the general U.S. population means of 50, indicating impaired HRQL. The mean baseline total IBDQ score was 126.5. At wk 30, pts in remission had the greatest increases from baseline in total IBDQ, PCS, and MCS. Changes for responders were smaller than those for pts in remission, but were greater than non-responders. All comparisons of the responder and remission groups vs the non-responder group were significant (p < 0.001, Table 1). Mean PCS (50.4) and MCS (51.8) for pts in remission at wk 30 were similar to the general U.S. population means.Table 1: Wk 30 Mean Total IBDQ, PCS, and MCS Scores and Change from BaselineNote: A 16 point change in IBDQ score or 2.5 point change in SF-36 PCS or MCS is considered clinically meaningful. Conclusions: Pts who achieved remission/response had significantly greater improvements in HRQL than non-responders. Pts in remission had HRQL scores similar to the general U.S. population.
Successful Management of the Sézary Syndrome (1974)

NEOPLASTIC thymus-derived (T) lymphocytes often have a characteristic tissue distribution.1 Unlike the abnormal bone-marrow-derived (B) lymphocytes of classic chronic lymphocytic leukemia and lymphosarcoma-cell leukemia,2 3 4 5 6 the leukemic T cells in the Sézary syndrome1 , 7 , 8 and in an unclassified leukemia9 preferentially infiltrate the skin and remarkably spare the bone marrow. Similarly, the neoplastic T cells in mycosis fungoides,1 a disorder probably related to the Sézary syndrome,10 11 12 characteristically localize in the skin, forming plaques and tumors.Many of the clinical sequelae of these T-cell neoplasms result from accumulation of malignant cells in extravascular sites; therefore, a prime therapeutic goal in their management is removal . . .
Reducing transaction aborts by looking to the future (2018)

Transactions are widely used in database engines and they becoming increasingly useful as a general synchronization technique for multicore machines [1]. Transactional systems allow a programmer to encapsulate multiple operations inside a transaction. All these operations appear to be executed atomically or not at all.
Hydrogen Bond Basicity Prediction for Medicinal Chemistry Design (2016)

Hydrogen bonding is discussed in the context of medicinal chemistry design. Minimized molecular electrostatic potential (Vmin) is shown to be an effective predictor of hydrogen bond basicity (pKBHX), and predictive models are presented for a number of hydrogen bond acceptor types relevant to medicinal chemistry. The problems posed by the presence of nonequivalent hydrogen bond acceptor sites in molecular structures are addressed by using nonlinear regression to fit measured pKBHX to calculated Vmin. Predictions are made for hydrogen bond basicity of fluorine in situations where relevant experimental measurements are not available. It is shown how predicted pKBHX can be used to provide insight into the nature of bioisosterism and to profile heterocycles. Examples of pKBHX prediction for molecular structures with multiple, nonequivalent hydrogen bond acceptors are presented.
Fabrication of 1 μm patterns on fused silica plates by laser-induced backside wet etching (LIBWE) (2003)

Laser-induced backside wet etching of fused silica plates using aqueous solutions of naphthalene-1,3,6-trisulfonic acid trisodium salt (Np) and pyranine (py) was performed upon KrF excimer laser irradiation at 248 nm. The two etching media show different etching behavior with changing laser fluence and medium concentrations. Well-defined line-and-space and grid micropatterns at 1 &mu;m scale were fabricated using an aqueous solution of Np and the etched pattern was free of debris and microcracks.
Incorporation of RG1 epitope concatemers into a self-adjuvanting Flagellin-L2 vaccine broaden durable protection against cutaneous challenge with diverse human papillomavirus genotypes (2017)

Vaccination with flagellin fused L2 multimers provided lasting (>1year) immunity without the need for an exogenous adjuvant. Inclusion of the L2 amino acid 17-38 region in such multi-HPV type fusions expanded the spectrum of protection.
Parental experiences of early pulmonary surveillance for children with cystic fibrosis: A research proposal for improved family psychosocial outcomes (2014)

The Australian Respiratory Early Surveillance Team for Cystic Fibrosis (AREST-CF) conducts an early surveillance program (ESP)for children with cystic fibrosis (CF). The program has the potential to modify long term health outcomes for children through early detection of lung disease, long before signs and symptoms are apparent. The program is aimed specifically at infants and preschool children (~3 months to 6 years) who undergo annual, detailed and relatively invasive surveillance. Although the ESP has been operating for 10 years, how parents experience their child undergoing early surveillance of this nature,and the psychosocial factors that moderate these experiences, is largely unknown. To date there are no published studies that have examined how parents cope with CF in the context of an ESP. Consequently, care pathways for improved mentalhealth and well-being for families involved with the ESP are incomplete. Therefore, the proposed study intends to explore parental experiences, including coping, related to their child’s involvement in the ESP, and to understand what psychosocial factors are important within the context of the ESP. Understanding parental experiences related to the ESP, how parents cope and identifying the psychosocial factors associated with these experiences is essential to inform CF centres and researchers considering adopting this program, and has the potential to inform intervention strategies that aim to support the family and foster healthy coping mechanisms.
Optical spin readout method in a quantum dot using the ac Stark effect (2015)

We propose a method to read out the spin state of an electron in a quantum dot in a Voigt geometry magnetic field using cycling transitions induced by the ac Stark effect. We show that cycling transitions can be made possible by a red-detuned, circularly polarized cw laser, which modifies the spin eigenstates and polarization selection rules via the ac Stark effect. A Floquet-Liouville supermatrix approach is used to calculate the time evolution of the density matrix under the experimental conditions of a spin readout operation. With an overall detection efficiency of 2.5%, the readout is a single-shot measurement with a fidelity of 76.2%.
Performance of Choline‐Based Deep Eutectic Solvents in the Extraction of Tocols from Crude Palm Oil (2015)

Abstract Deep eutectic solvents are emerging green solvents that have potential in many separation processes. This study investigates the performance of choline‐based deep eutectic solvents in the extraction of tocopherols and tocotrienols (collectively known as tocols) from palm oil, a major natural source of tocols. Deep eutectic solvents comprised of choline chloride salt and selected carboxylic acids as hydrogen bond donors were prepared and used in the extraction of tocols from crude palm oil by liquid–liquid extraction. Tocols concentration in the extracted product was at least double that in the control (8671 mg/kg compared to 3285 mg/kg, respectively). Increasing the amount of the deep eutectic solvents increased the tocols concentration in the extracted product up to 18,525 mg/kg, but the yields lowered from 4 % to less than 1 %. The tocols profile was significantly improved by the increase of the tocotrienols fraction in the products from 80.8 to 99.8 %. This study showed that unique interaction between the selected deep eutectic solvents with the tocols make it possible to selectively separate individual tocols in palm oil, where products with fractions rich in tocotrienols and low in tocopherols (particularly α‐tocopherol) are favorable.
First principles molecular dynamics simulations for amorphous HfO2 and Hf1-xSixO2 systems (2005)

Amorphous phases of HfO2 and Hf1–xSixO2 were obtained using the Projector Augmented Plane Wave method through the melt and quench technique. For the pure HfO2 system, several pore channels appear in the structures. Changes to x in the Hf1–xSixO2 were also studied. As the concentration of Si increases, the size of the pore channels increases, much space appears and two-fold oxygen atoms in-crease. By calculating the heat of formation energy, it was found that phase separation between amor-phous HfO2 and SiO2 occurs at x&amp;gt;0.1. Key words: HfO2; Hf1–xSixO2; amorphous; first principles; molecular dynamics 1.
Reflectance changes at 1° temporal from the fovea. (2015)

&lt;p&gt;The dark gray lines indicate the reflectance changes at 1° temporal from the fovea. The black line shows the low-pass filtered data at 0.008-Hz. The dotted line indicates the baseline. The light gray zone indicates the period of light stimulation. Cone reflectances increased during light stimulation and had two peaks in all subjects. Subject 2 underwent the repeatability experiments (S2 #1 and S2 #2).&lt;/p&gt;
Microbiota organization is a distinct feature of proximal colorectal cancers (2014)

Environmental factors clearly affect colorectal cancer (CRC) incidence, but the mechanisms through which these factors function are unknown. One prime candidate is an altered colonic microbiota. Here we show that the mucosal microbiota organization is a critical factor associated with a subset of CRC. We identified invasive polymicrobial bacterial biofilms (bacterial aggregates), structures previously associated with nonmalignant intestinal pathology, nearly universally (89%) on right-sided tumors (13 of 15 CRCs, 4 of 4 adenomas) but on only 12% of left-sided tumors (2 of 15 CRCs, 0 of 2 adenomas). Surprisingly, patients with biofilm-positive tumors, whether cancers or adenomas, all had biofilms on their tumor-free mucosa far distant from their tumors. Bacterial biofilms were associated with diminished colonic epithelial cell E-cadherin and enhanced epithelial cell IL-6 and Stat3 activation, as well as increased crypt epithelial cell proliferation in normal colon mucosa. High-throughput sequencing revealed no consistent bacterial genus associated with tumors, regardless of biofilm status. However, principal coordinates analysis revealed that biofilm communities on paired normal mucosa, distant from the tumor itself, cluster with tumor microbiomes as opposed to biofilm-negative normal mucosa bacterial communities also from the tumor host. Colon mucosal biofilm detection may predict increased risk for development of sporadic CRC.
Comparison of the accuracy of various transformations from multi-band images to reflectance spectra (2002)

This report provides a comparative study of the spectral and colorimetric accuracy of various transformations from multi-band digital signals to spectral reflectance. The multiband channels were obtained by multi-channel visible-spectral imaging (MVSI) using a monochrome CCD and two different filtering systems. In the first system we used a liquid-crystal tunable filter (LCTF) capturing 31 narrow-band channels. We also used a filter wheel with a set of 6 glass filters imaging with and without an extra Wratten absorption filter giving a total of 12 channels. Four different mathematical methods were tested to derive reflectance spectra from digital signals: pseudo-inverse, eigenvector analysis, modified-discrete sine transformation (MDST) and non-negative least squares (NNLS). We also considered two different approaches to sampling the digital signals; in one approach we averaged the digital counts.
ChemInform Abstract: A New, One‐Step Synthesis of 1‐Heteroaryl‐2‐alkylaminoethanols. (2010)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Use of monoclonal antibodies to protein F of Pseudomonas aeruginosa as opsonins for phagocytosis by macrophages (1987)

Five protein F-specific monoclonal antibodies were found to opsonize Pseudomonas aeruginosa for complement-independent phagocytosis by unelicited mouse peritoneal macrophages, mouse macrophage cell line P388D1, and human monocyte-derived macrophages. Immunoglobulin G1 antibodies seemed to be a preferred isotype.
Insulin-like growth factor-I supports proliferation of autocrine thymic lymphoma cells with a pre-T cell phenotype. (1990)

We have studied the phenotypic characteristics and growth properties of murine T lymphoma cell lines derived from primary x-ray-induced thymic lymphomas at the earliest stage at which they can be detected, and well before spreading to other organs has occurred. These cell lines serve as model systems for the earliest events in T cell lymphoma induction, before tumor cell progression and spreading to other organs. We find that primary x-ray-induced T cell lymphoma lines have phenotypic characteristics of thymic pre-T cells and show no proliferative response to any of the IL tested nor to other hematopoietic growth factors. However, they do proliferate in response to insulin-like growth factor I (IGF-I) and to a small autocrine peptide distinct from IGF-I, which we term lymphoma growth factor. One of the earliest lesions in T cell lymphoma induction may therefore be an inhibition of differentiation at one of several specific points. In its early stages, T lymphoma cell growth may be restricted to an environment where local concentrations of specific growth factors such as IGF-I or lymphoma growth factor are sufficiently high.
A stochastic view on surface inhomogeneity of nanoparticles (2019)

The interactions between and with nanostructures can only be fully understood when the functional group distribution on their surfaces can be quantified accurately. Here we apply a combination of direct stochastic optical reconstruction microscopy (dSTORM) imaging and probabilistic modelling to analyse molecular distributions on spherical nanoparticles. The properties of individual fluorophores are assessed and incorporated into a model for the dSTORM imaging process. Using this tailored model, overcounting artefacts are greatly reduced and the locations of dye labels can be accurately estimated, revealing their spatial distribution. We show that standard chemical protocols for dye attachment lead to inhomogeneous functionalization in the case of ubiquitous polystyrene nanoparticles. Moreover, we demonstrate that stochastic fluctuations result in large variability of the local group density between particles. These results cast doubt on the uniform surface coverage commonly assumed in the creation of amorphous functional nanoparticles and expose a striking difference between the average population and individual nanoparticle coverage.
Supplementary 3 from A Novel Anti-CD22 Anthracycline-Based Antibody–Drug Conjugate (ADC) That Overcomes Resistance to Auristatin-Based ADCs (2023)

&lt;p&gt;Supplementary 3. Cell lines derived from xenograft tumors resistant to anti-CD22-vc-MMAE were resistance to anti-CD22-vc-MMAE&lt;/p&gt;
Photochemical Characterization and Optimization of Argon Ion Laser-Induced Fluorescence Detection of o-Phthalaldehyde/.beta.-Mercaptoethanol-Labeled Amino Acids and .gamma.-Glutamyl Peptides in Liquid Chromatography: Ultratrace Analysis with Neurobiological Samples (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhotochemical Characterization and Optimization of Argon Ion Laser-Induced Fluorescence Detection of o-Phthalaldehyde/.beta.-Mercaptoethanol-Labeled Amino Acids and .gamma.-Glutamyl Peptides in Liquid Chromatography: Ultratrace Analysis with Neurobiological SamplesOwe. Orwar, Mats. Sandberg, Ingemar. Jacobson, Mikael. Sundahl, and Staffan. FolestadCite this: Anal. Chem. 1994, 66, 24, 4471–4482Publication Date (Print):December 15, 1994Publication History Published online1 May 2002Published inissue 15 December 1994https://pubs.acs.org/doi/10.1021/ac00096a013https://doi.org/10.1021/ac00096a013research-articleACS PublicationsRequest reuse permissionsArticle Views118Altmetric-Citations18LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts