gebhart/patrepeval huggingface dataset:
Yeast Oligo-Mediated Genome Engineering (YOGE) (2013)

High-frequency oligonucleotide-directed recombination engineering (recombineering) has enabled rapid modification of several prokaryotic genomes to date. Here, we present a method for oligonucleotide-mediated recombineering in the model eukaryote and industrial production host Saccharomyces cerevisiae , which we call yeast oligo-mediated genome engineering (YOGE). Through a combination of overexpression and knockouts of relevant genes and optimization of transformation and oligonucleotide designs, we achieve high gene-modification frequencies at levels that only require screening of dozens of cells. We demonstrate the robustness of our approach in three divergent yeast strains, including those involved in industrial production of biobased chemicals. Furthermore, YOGE can be iteratively executed via cycling to generate genomic libraries up to 10 (5) individuals at each round for diversity generation. YOGE cycling alone or in combination with phenotypic selections or endonuclease-based negative genotypic selections can be used to generate modified alleles easily in yeast populations with high frequencies.
The History Tablecloth (Overview) (2004)

The History Tablecloth is an interactive device developed by the Interaction Research Studio as part of the Equator project, a six-year Interdisciplinary Research Collaboration, funded by the Engineering and Physical Sciences Research Council (EPSRC). During the project, the piece was loaned to numerous households for field study and has since been widely exhibited.
Biological Macromolecules and Assemblies (1985)

Book Review| October 01 1985 Biological Macromolecules and Assemblies Biological Macromolecules and Assemblies. FRANCES A. JURNAK, ALEXANDER McPHERSON. John Wiley and SonsNew York 1985 pp. 508, £6.70 S. NEIDLE S. NEIDLE Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1985) 13 (5): 971–972. https://doi.org/10.1042/bst0130971a Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Cite Icon Cite Get Permissions Citation S. NEIDLE; Biological Macromolecules and Assemblies. Biochem Soc Trans 1 October 1985; 13 (5): 971–972. doi: https://doi.org/10.1042/bst0130971a Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu nav search search input Search input auto suggest search filter All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1985 Biochemical Society1985 Article PDF first page preview Close Modal You do not currently have access to this content.
A Protocol for the Reproducible Silanization of Mica Validated by Sum Frequency Spectroscopy and Atomic Force Microscopy (2000)

A reaction protocol has been developed which when rigorously adhered to leads to the reproducible formation of complete, well-ordered monolayers of octadecylsiloxane (ODS) on mica. The complementary information provided by atomic force microscopy (AFM), sum frequency spectroscopy (SFS), and contact angle measurements has been used to validate the protocol and to characterize the structure and coverage of the films. The essential prerequisites for reproducibility were found to be fresh octadecyltrichlorosilane (OTS), a clean reaction environment, controlled surface hydration, and high-purity anhydrous solvents. During the reaction itself the well-recognized parameters of reaction temperature and the degree of bulk hydration required strict regulation. Reactions were performed at 19 °C, and as expected, a mixed ODS growth regime was observed consisting of ordered islands and a disordered diffuse phase. The formation of complete, well-ordered monolayers was favored by low OTS concentrations and extended reaction times of up to 24 h.
A One‐Dimensional Zirconium Hydroxyfluoride, [Zr(OH)2F3][enH]. (2006)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
CD40L+ CD4+ memory T cells migrate in a CD62P-dependent fashion into reactive lymph nodes and license dendritic cells for T cell priming (2008)

There is growing evidence that the maturation state of dendritic cells (DCs) is a critical parameter determining the balance between tolerance and immunity. We report that mouse CD4(+) effector memory T (T(EM)) cells, but not naive or central memory T cells, constitutively expressed CD40L at levels sufficient to induce DC maturation in vitro and in vivo in the absence of antigenic stimulation. CD4(+) T(EM) cells were excluded from resting lymph nodes but migrated in a CD62P-dependent fashion into reactive lymph nodes that were induced to express CD62P, in a transient or sustained fashion, on high endothelial venules. Trafficking of CD4(+) T(EM) cells into chronic reactive lymph nodes maintained resident DCs in a mature state and promoted naive T cell responses and experimental autoimmune encephalomyelitis (EAE) to antigens administered in the absence of adjuvants. Antibodies to CD62P, which blocked CD4(+) T(EM) cell migration into reactive lymph nodes, inhibited DC maturation, T cell priming, and induction of EAE. These results show that T(EM) cells can behave as endogenous adjuvants and suggest a mechanistic link between lymphocyte traffic in lymph nodes and induction of autoimmunity.
GMTI Direction of Arrival Measurements from Multiple Phase Centers (2015)

Ground Moving Target Indicator (GMTI) radar attempts to detect and locate targets with unknown motion. Very slow-moving targets are difficult to locate in the presence of surrounding clutter. This necessitates multiple antenna phase centers (or equivalent) to offer independent Direction of Arrival (DOA) measurements. DOA accuracy and precision generally remains dependent on target Signal-to-Noise Ratio (SNR), Clutter-toNoise Ratio (CNR), scene topography, interfering signals, and a number of antenna parameters. This is true even for adaptive techniques like Space-Time-AdaptiveProcessing (STAP) algorithms.
Food log by analyzing food images (2008)

In this paper, a food-logging system that can distinguish food images from other images, analyze the food balance, and visualize the log is presented. The image processing is based on feature vectors consisting of color histograms, DCT coefficients, detected image patterns and so forth. Support Vector Machine (SVM) was used to detect food images and to analyze the food balance. Experimental results show that the food image extraction presents above 88% of accuracy and the food balance estimation is achieved with more than 73% of accuracy.
Erratum: In vivo single-molecule imaging of syntaxin1A reveals polyphosphoinositide- and activity-dependent trapping in presynaptic nanoclusters (2017)

Nature Communications 7: Article number: 13660 (2016); Published: 16 December 2016; Updated: 9 February 2017 This Article was originally published with an incorrect publication date. The paper was due to be published on 3 Jan 2017, but due to an error was published earlier on the 16 Dec 2016. The publication date in both the PDF and HTML versions of the paper has been updated to reflect this.
Rücktitelbild: A Highly‐Ordered 3D Covalent Fullerene Framework (Angew. Chem. 26/2015) (2015)

Ein kovalentes 3D-Fullerengerüst …︁ …︁, dessen Struktur auf sechsfach funktionalisierten Fullerenbausteinen mit Oktaedersymmetrie beruht, wird von T. Bein et al. in der Zuschrift auf S. 7687 ff. vorgestellt. Jedes Fulleren in der hoch geordneten Struktur ist vom nächstgelegenen durch sechs funktionelle Gruppen getrennt, und die Mesoporosität wird über eine templatgesteuerte verdampfungsinduzierte Selbstorganisation mit einem Blockcopolymer kontrolliert. Die TEM-Bilder im Hintergrund zeigen das periodische poröse Fullerengerüst.
Accurate Coulometric Quantification of Hydrogen Absorption in Palladium Nanoparticles and Thin Films (2018)

We report here an electrochemical method for precise and accurate quantification of hydrogen absorption in palladium materials. We demonstrate that conventional chronocoulometry over-reports adsorbed hydrogen due to charge from the accompanying hydrogen oxidation reaction (HOR). We designed and built a bespoke electrochemical flow cell that mitigates the concurrent HOR reaction and consequently provides improved accuracy and reproducibility relative to other existing electrochemical techniques. The efficacy of this technique is demonstrated experimentally for a series of palladium sample types: a 100 nm electron-beam deposited thin film, a 20 μm electrodeposited palladium film, a casting of 21 nm edge-length cubic nanoparticles, and a casting of 27 nm edge-length octahedral nanoparticles. We contend that this method is the most effective for measuring hydrogen uptake in different palladium samples.
A multidisciplinary consensus on dehydration: definitions, diagnostic methods and clinical implications (2019)

<b>Background:</b> Dehydration appears prevalent, costly and associated with adverse outcomes. We sought to generate consensus on such key issues and elucidate need for further scientific enquiry. <b>Materials and methods:</b> A modified Delphi process combined expert opinion and evidence appraisal. Twelve relevant experts addressed dehydration's definition, objective markers and impact on physiology and outcome. <b>Results:</b> Fifteen consensus statements and seven research recommendations were generated. Key findings, evidenced in detail, were that there is no universally accepted definition for dehydration; hydration assessment is complex and requires combining physiological and laboratory variables; "dehydration" and "hypovolaemia" are incorrectly used interchangeably; abnormal hydration status includes <i>relative</i> and/or <i>absolute</i> abnormalities in body water and serum/plasma osmolality (pOsm); raised pOsm usually indicates dehydration; direct measurement of pOsm is the gold standard for determining dehydration; pOsm >300 and ≤280 mOsm/kg classifies a person as hyper or hypo-osmolar; outside extremes, signs of adult dehydration are subtle and unreliable; dehydration is common in hospitals and care homes and associated with poorer outcomes. <b>Discussion:</b> Dehydration poses risk to public health. Dehydration is under-recognized and poorly managed in hospital and community-based care. Further research is required to improve assessment and management of dehydration and the authors have made recommendations to focus academic endeavours. Key messages Dehydration assessment is a major clinical challenge due to a complex, varying pathophysiology, non-specific clinical presentations and the lack of international consensus on definition and diagnosis. Plasma osmolality represents a valuable, objective surrogate marker of hypertonic dehydration which is underutilized in clinical practice. Dehydration is prevalent within the healthcare setting and in the community, and appears associated with increased morbidity and mortality.
A new conduction model for polycrystalline silicon films (1981)

A new quantitative electrical model is introduced to solve earlier modeling inadequacies in polycrystalline silicon films. An analytical J-V expression is developed in normalized closed form, which includes the thermionic field emission through a space-charge potential barrier and through a grain-boundary scattering potential barrier and the thermionic emission over these barriers. The modeling validity has been verified experimentally for films with grain sizes of 230 to 1220 Å, doping concentrations from 1 × 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">16</sup> to 8 × 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">19</sup> cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-3</sup> and over a temperature range from -176° to 144°C.
Identifying Foreign Person Names in Chinese Text (2008)

Foreign name expressions written in Chinese characters are difficult to recognize since the sequence of characters represents the Chinese pronunciation of the name. This paper suggests that known English or German person names can reliably be identified on the basis of the similarity between the Chinese and the foreign pronunciation. In addition to locating a person name in the text and learning that it is foreign, the corresponding foreign name is identified, thus gaining precious additional information for cross-lingual applications. This idea is implemented as a statistical module into a rule-based named entity recognition system. 1.
ChemInform Abstract: Preparation of Optically Active Functionalized cis‐Δ6‐1‐Octalones. (1985)

Abstract Ausgehend von (+)‐Nopinon (I), für dessen Darstellung in optisch reiner Form ein Verfahren beschrieben wird, gelingt entsprechend den Angaben des Formelschemas die Synthese der Verbindung (IX) mit drei funktionalisierten chiralen Zentren.
Cancer Radioimmunotherapy (2011)

Targeting of radionuclides with antibodies, or radioimmunotherapy, has been an active field of research spanning nearly 50 years, evolving with advancing technologies in molecular biology and chemistry, and with many important preclinical and clinical studies illustrating the benefits, but also the challenges, which all forms of targeted therapies face. There are currently two radiolabeled antibodies approved for the treatment of non-Hodgkin lymphoma, but radioimmunotherapy of solid tumors remains a challenge. Novel antibody constructs, focusing on treatment of localized and minimal disease, and pretargeting are all promising new approaches that are currently under investigation.
Highly Efficient Green Organic Light-Emitting Diodes Containing Luminescent Three-Coordinate Copper(I) Complexes (2011)

A series of highly emissive three-coordinate copper(I) complexes, (dtpb)Cu(I)X [X = Cl (1), Br (2), I (3); dtpb =1,2-bis(o-ditolylphosphino)benzene], were synthesized and investigated in prototype organic light-emitting diodes (OLEDs). 1-3 showed excellent photoluminescent performance in both degassed dichloromethane solutions [quantum yield (Φ) = 0.43-0.60; lifetime (τ) = 4.9-6.5 μs] and amorphous films (Φ = 0.57-0.71; τ = 3.2-6.1 μs). Conventional OLEDs containing 2 in the emitting layer exhibited bright green luminescence with a current efficiency of 65.3 cd/A and a maximum external quantum efficiency of 21.3%.
Systemic Bevacizumab for Treatment of Respiratory Papillomatosis: International Consensus Statement (2021)

5 Laryngoscope, 131:E1941-E1949, 2021.
An integrated environment for modeling, simulation, digital signal processing, and control (1996)

An integrated laboratory for system modeling, simulation, real-time digital signal processing, and control is being developed at Bucknell University for undergraduate electrical engineering education. The laboratory bridges the gap between software simulation and testing of actual systems through a common visual programming interface. Students can explore the iterative process of developing a model and then refining the model until computer simulation results agree with experimental measurements. A liquid level control system is presented to illustrate the features of the laboratory environment. The key components of the laboratory are networked digital signal processing (DSP) hardware units and a simulation software package. The simulation software runs on workstations, and all of the laboratory equipment (including the DSP hardware) is connected to the Internet.
Figure S3 from Efficacy of Affibody-Based Ultrasound Molecular Imaging of Vascular B7-H3 for Breast Cancer Detection (2023)

&lt;p&gt;ABYB7-H3 positively stains B7-H3 expressed in the tissue sections from human breast cancer patients.&lt;/p&gt;
Metro: An Analysis Toolkit for Template Semantics (2006)

We describe the Metro toolkit, which supports software modelling and analysis for requirements notations that have configurable semantics. Metro is based on a formalism, called template semantics, which structures the operational semantics of a family of notations as a predefined parameterized template that is instantiated with user-provided parameter values. Thus, the semantics of a single notation can be expressed succinctly as a set of parameter values to this template. The Metro toolkit takes as input a specification and a set of template-parameter values, and it produces an analyzable model. The toolkit can either translate the specification into the input language of an existing model checker (e.g., SMV), or compile the specification into a more primitive form (e.g., logic, BDDs) that is suitable for analysis. MagicDraw is used as a front-end for editing specifications and animating SMV-generated counterexamples. 1
The TCR Repertoire of an Immunodominant CD8+ T Lymphocyte Population (2001)

The TCR repertoire of an epitope-specific CD8(+) T cell population remains poorly characterized. To determine the breadth of the TCR repertoire of a CD8(+) T cell population that recognizes a dominant epitope of the AIDS virus, the CD8(+) T cells recognizing the tetrameric Mamu-A*01/p11C(,CM) complex were isolated from simian immunodeficiency virus (SIV)-infected Mamu-A*01(+) rhesus monkeys. This CD8(+) T cell population exhibited selected usage of TCR V beta families and complementarity-determining region 3 (CDR3) segments. Although the epitope-specific CD8(+) T cell response was clearly polyclonal, a dominance of selected V beta(+) cell subpopulations and clones was seen in the TCR repertoire. Interestingly, some of the selected V beta(+) cell subpopulations and clones maintained their dominance in the TCR repertoire over time after infection with SIV of macaques. Other V beta(+) cell subpopulations declined over time in their relative representation and were replaced by newly evolving clones that became dominant. The present study provides molecular evidence indicating that the TCR repertoire shaped by a single viral epitope is dominated at any point in time by selected V beta(+) cell subpopulations and clones and suggests that dominant V beta(+) cell subpopulations and clones can either be stable or evolve during a chronic infection.
Secondary emission of strain-induced dopant contrast in the source/drain regions of metal-oxide-semiconductor devices (2006)

Secondary electron (SE) imaging with scanning electron microscopy has been used for two-dimensional dopant profiling. However, the mechanism of dopant contrast is still not yet understood. Here we propose another significant contribution from interface strain for the source/drain regions in p- and n-type metal-oxide-semiconductor (MOS) devices. The results show that the width of the dopant profile by SE imaging agrees well with the strain profile by dark-field technique with transmission electron microscopy. We demonstrate that the dopant contrast of the p-MOS device by SE imaging is higher than a test wafer by 45%. The enhanced SE signals for both p-MOS and n-MOS devices are caused by band bending through CoSi2-induced strain.
Massively Parallel Nearest Neighbor Queries for Dynamic Point Clouds on the GPU (2009)

We introduce a parallel algorithm to solve approximate and exact nearest neighbor queries on the GPU, exploiting its massively parallel processing power. Both data structure construction and nearest neighbor queries are performed on the GPU, avoiding memory copies from system memory to device memory. This algorithm achieves real-time performance, enabling its usage in dynamic scenarios, by minimizing the sorting comparisons needed for a large K value. The underlying data structure for spatial subdivision handles 3D points and is based on grid spatial hashing. Users can specify the grid size interactively. Comparisons were done with other nearest neighbor algorithms implemented on both CPU and GPU. Our approach clearly surpasses CPU implementations regarding processing time, while it presents a competitive solution to GPU ones. Real-time results were obtained with ANN searches (K = 10) for data sets up to 163K points and the potential of our algorithm is demonstrated through a point-based rendering application.
Processing and editing of faces using a measurement-based skin reflectance model (2006)

No abstract available.
ERF Nuclear Shuttling, a Continuous Monitor of Erk Activity That Links It to Cell Cycle Progression (2004)

The ets domain transcriptional repressor ERF is an effector of the receptor tyrosine kinase/Ras/Erk pathway, which, it has been suggested, is regulated by subcellular localization as a result of Erk-dependent phosphorylation and is capable of suppressing cell proliferation and ras-induced tumorigenicity. Here, we analyze the effect of ERF phosphorylation on nuclear import and export, the timing of its phosphorylation and dephosphorylation in relation to its subcellular location, Erk activity, and the requirements for ERF-induced cell cycle arrest. Our findings indicate that ERF continuously shuttles between the nucleus and the cytoplasm and that both phosphorylation and dephosphorylation of ERF occur within the nucleus. While nuclear import is not affected by phosphorylation, ERF nuclear export and cytoplasmic release require multisite phosphorylation and dephosphorylation. ERF export is CRM1 dependent, although ERF does not have a detectable nuclear export signal. ERF phosphorylation and export correlate with the levels of nuclear Erk activity. The cell cycle arrest induced by nonphosphorylated ERF requires the wild-type retinoblastoma protein and can be suppressed by overexpression of cyclin. These data suggest that ERF may be a very sensitive and constant sensor of Erk activity that can affect cell cycle progression through G(1), providing another link between the Ras/Erk pathway and cellular proliferation.
Cobalt-mediated modification of oxide surfaces with redox-active molecules (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCobalt-mediated modification of oxide surfaces with redox-active moleculesKaimin Chen, Brian R. Herr, Elizabeth T. Singewald, and Chad A. MirkinCite this: Langmuir 1992, 8, 11, 2585–2587Publication Date (Print):November 1, 1992Publication History Published online1 May 2002Published inissue 1 November 1992https://doi.org/10.1021/la00047a001Request reuse permissionsArticle Views179Altmetric-Citations7LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (374 KB) Get e-Alertsclose Get e-Alerts