gebhart/patrepeval huggingface dataset:
Extracellular ATP induces cytoplasmic and nuclear Ca2+ transients via P2Y2 receptor in human biliary epithelial cancer cells (Mz-Cha-1). (2012)

Extracellular nucleotides such as adenosine triphosphate (ATP) play a role in biliary epithelial cell function. Since nucleotide receptors are potential targets for various diseases related to epithelial cell dysfunction and cancer, the purpose of this study was to investigate the expression and to functionally characterize the nucleotide receptor subtypes in biliary epithelial cancer cells (Mz-Cha-1). Extracellular ATP dose-dependently resulted in an intracellular Ca(2+) increase (mean effective concentration (EC(50)) 40 μM). Uridine triphosphate (UTP) produced a similar Ca(2+) response and cross-desensitation was observed. The rank order of tested agonists was ATP=UTP>> adenosine>ADP=AMP>α,β-methylene-ATP. This confirms the functional expression of purinoceptor P2Y2 and P2Y4 in biliary epithelial cancer cell membranes. mRNAs for P2Y1, P2Y2, P2Y4 and P2Y6 purinergic receptor subtypes were found, whereas western blot analysis suggested only the expression of P2Y2 receptors. Confocal imaging and nuclear staining was used to compartmentalize ATP-induced cytosolic and nuclear Ca(2+)-transients, indicating a role for secretory ATP in regulating nuclear function, by increasing nuclear Ca(2+) concentrations. These data define the expression profile of P2Y receptors on human biliary epithelial cancer cells and indicate P2Y2 receptors as being potential targets in new treatment strategies for biliary cancer.
Cryo-FIB/SEM Investigation of Mechanism of Frost Formation on Lubricant-Impregnated Surfaces (2013)

Extended abstract of a paper presented at Microscopy and Microanalysis 2013 in Indianapolis, Indiana, USA, August 4 – August 8, 2013.
ChemInform Abstract: A Novel Annelation Process Involving Perfluorocycloalkene Derivatives. (1993)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
A Simple Approach to MXene Micropatterning from Molecularly Driven Assembly (2021)

Here, a micropatterning strategy is demonstrated to achieve stable and selective MXene adsorption through the molecularly driven assembly. MXene flakes were assembled by strong interaction with a silicon substrate, which was functionalized by microcontact printing (μCP) to create an active surface. A clear micropattern was observed by scanning electron microscopy showing uniform coverage of MXene flakes. Atomic force microscopy revealed a pattern thickness of around 50 nm, much thinner than the patterns obtained by direct μCP. The obtained micropattern presents good stability against rinsing and sonication. X-ray photoelectron spectroscopy shows that this stability can be attributed to strong covalent bonding between MXene and active molecules on a silicon substrate. The sheet resistance of the as-formed MXene layer was measured at around 154.67 (Ω/□), which is lower than those of other published techniques with a similar thickness of around 50 nm. This method can achieve a well-defined MXene pattern around the sub-100 μm scale without requiring prior MXene surface modification. Therefore, MXene can retain its intrinsic surface property, allowing further molecule adsorption as a sensing platform. Moreover, this patterning technique does not require complicated control of ink preparation and offers possible application on a substrate of any geometry with few layers of thickness.
Cell viability and shock wave amplitudes in the endothelium of porcine cornea exposed to ultrashort laser pulses (2016)

International audience
Cu Electrodeposition on Resistive Substrates in Alkaline Chemistry: Effect of Current Density and Wafer RPM (2011)

A wafer-scale wet alkaline seed electrodeposition process directly on resistive substrates, such as PVD RuTa and CVD Co films as thin as 2 and 5 nm respectively, is studied. The effect of the directly plated stack composition and thicknesses, plating waveform, rotation speed and applied current on the plating rate and morphology of the deposited copper is reported. The alkaline deposition process is directly compared to the acid deposition process on the resistive RuTa substrate, while the thin Co films undergo to dissolution in acidic chemistries. In the case of the alkaline chemistry, since the deposition is less mass transport dependent and less sensitive to preferential nucleation due to terminal effect, the presence of Cu complexing agents results in a more uniform distribution of the Cu ions along the wafer surface translating in a higher throwing power with respect to acid plating baths.
Stimulation by calcium and barium of somatostatin release. Evidence for lower sensitivity of D‐ vis‐à‐vis B‐ and A‐cells (1984)

To address the question whether a "second messenger" function of calcium differs between D-cells and other cells of the endocrine pancreas, we compared effects of calcium and barium (a calcium substitute) on somatostatin secretion to effects on insulin and glucagon secretion from the perfused pancreas of the rat. 6.5 mmol/l of calcium, when administered early during perfusion, failed to stimulate somatostatin release. 0.05 mmol/l of barium, when added to calcium-deprived media failed to affect somatostatin secretion while 0.5 induced a slight and 2.0 mmol/l a marked and sustained response. Barium-induced insulin release was left-shifted in relation to the somatostatin response, since 0.05 mmol/l of barium stimulated and 0.5 mmol/l evoked a near-maximal insulin response. All concentrations of barium evoked diphasic glucagon responses, i.e. a small (1 min) stimulation followed by sustained inhibition. Addition of 0.5 mmol/l of EGTA to calcium-deprived media abolished D- as well as B- and A-cell secretion. Reintroduction of 0.5-6.5 mmol/l of calcium stimulated somatostatin release; the secretory response was proportionate to the calcium concentration. In contrast, addition of calcium stimulated insulin and glucagon secretion maximally already at 0.5 mmol/l of calcium. We conclude that the D-cell is less sensitive than B- and A-cells to a regulatory effect on secretion exerted by extracellular calcium or barium.
ChemInform Abstract: Synthesis and Properties of Optically Active Phosphine‐Boranes Possessing an l‐Menthyloxy Group. (1995)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Autonomic Activity and Surgical Flow Disruptions in Healthcare Providers during Cardiac Surgery (2020)

Cardiac surgery represents a complex sociotechnical environment relying on a combination of technical and non-technical team-based expertise. Surgical flow disruptions (SFDs) may be influenced by a variety of sources, including social, environmental, and emotional factors affecting healthcare providers (HCPs). Many of these factors can be readily observed, except for emotional factors (i.e. distress), which represents an underappreciated yet critical source of SFDs. The aim of this study was to demonstrate the sensitivity of autonomic activity metrics to detect an SFD during cardiac surgery. We integrated heart rate variability (HRV) analysis with observation-based annotations to allow data triangulation. Following a critical medication administration error by the anesthesiologist in-training, data sources were consulted to identify events precipitating this near-miss event. Using <i>pyphysio</i>, an open-source physiological signal processing package, we analyzed the attending anesthesiologists' HRV, specifically the low frequency (LF) power, high frequency (HF) power, LF/HF ratio, standard deviation of normal-to-normal (SDNN), and root mean square of the successive differences (RMSSD) as indicators of ANS activity. A heightened SNS response in the attending anesthesiologists' physiological arousal was observed as elevations in LF power and LF/HF ratio, as well as depressions in HF power, SDNN, and RMSSD prior to the near-miss event. The attending anesthesiologist subjectively confirmed a state of high distress induced by task-irrelevant environmental factors during this time. Qualitative analysis of audio/video recordings objectively revealed that the autonomic nervous system (ANS) activation detected was temporally associated with an argument over operating room management. This study confirms that it is possible to recognize detrimental psychophysiological influences in cardiac surgery procedures via advanced HRV analysis. To our knowledge, ours is the first such case demonstrating ANS activity coinciding with strong self-reported emotion during live surgery using HRV. Despite extensive experience in the cardiac OR, transient but intense emotional changes may have the potential to disrupt attention processes in even the most experienced HCP. A primary implication of this work is the possibility to detect real-time ANS activity, which could enable personalized interventions to proactively mitigate downstream adverse events. Additional studies on our large database of surgical cases are underway and new studies are actively being planned to confirm this preliminary observation.
Electrocatalysis: Wafer Scale Phase‐Engineered 1T‐ and 2H‐MoSe2/Mo Core–Shell 3D‐Hierarchical Nanostructures toward Efficient Electrocatalytic Hydrogen Evolution Reaction (Adv. Mater. 44/2016) (2016)

On page 9831, Z. M. Wang, Y.-L. Chueh and co-workers present wafer scale phase-engineered 1T- and 2H- MoSe2/Mo core-shell 3D-hierarchical nanostructures, toward efficient electrocatalytic hydrogen evolution reaction with excellent Tafel slope as low as 34.7 mV dec−1, which is close to the theoretical value of 29 mV dec−1 dominated by a Volmer-Tafel reaction.
Isolation and Initial Characterization of Nonhuman Primate Amniotic Fluid-Derived Stem Cells (2013)

Regenerative medicine promises to address the organ shortage by engineering replacement cells, tissues and organs using adult, progenitor, or human embryonic stem cells. However, embryonic stem cells can form teratomas and bone marrow-derived progenitor cells have limited expansion capabilities. We recently described a subpopulation of c-kit human amniotic fluid-derived stem (hAFS) cells that are highly expansive, do not form teratomas and can yield differentiated cell derivatives corresponding to the three germ layers. To this end, and because nonhuman primates (NHP) represent a critical step towards pre-clinical testing of the AFS technology, the goal of this study was to determine if AFS cells could be isolated from amniotic samples of monkeys. Expansive cell cultures were derived from 12/28 amniotic fluid samples of cynomolgus monkey. Cells were isolated and expanded in Chang’s media and c-kit cells were isolated, further expanded and clonal populations obtained. The putative AFS cells were characterized as to their karyotype, ability to expand, identification of cell-surface and nuclear markers (qPCR) and ability to differentiate to mesodermal lineages such as bone, muscle, fat, cartilage and endothelial cells. Highly expansive multipotent cells were isolated most readily from the second trimester of pregnancy (8/15 samples, or 53%), and less so from either the first (1/3, or 33%), or third (3/10, or 30%) trimester. Cell were diploid in all cases and were consistently expanded through at least 20 passages (10 passages for the 3rd trimester cells) without losing their proliferation potential, as confirmed by cell cycle analysis (G1, G2, S phase) and doubling times. The AFS cells had low-level expression of pluripotentiality markers (Oct4, Nanog, SOX2) compared to NHP embryonic stem cells, while expressing markers similar to that of adult mesodermal-lineage cells (CD44, CD90, CD73 and CD105). nhpAFS cells express the major, but not minor, histocompatability antigen and were consistently differentiated into adipogenic, osteogenic, myogenic, chondrocytic and endothelial lineages. Because of their robust expansion capabilities and ability to differentiate into several mesodermal lineages, it is concluded that nhpAFS cells, similar to hAFS cels, have the characteristics needed to bioengineer a wide-variety of mesodermal-cell containing organs and tissues. Published by www.inter-use.com. Available online Dec. 1
Internalization of Escherichia coli by human renal epithelial cells is associated with tyrosine phosphorylation of specific host cell proteins (1997)

Human renal epithelial cells are capable of internalizing Escherichia coli regardless of whether the bacteria are isolated from individuals with pyelonephritis or from healthy volunteers. In this study, we investigated the role of host cell tyrosine kinase activity in internalization. We found that internalization of both fecal and pyelonephritis isolates is blocked by tyrosine kinase inhibitors. We found increased intensity of two tyrosine-phosphorylated proteins, with relative mobilities of approximately 123,000 and 110,000, in Western blots of extracts from human renal epithelial cells infected with E. coli. The increased intensity of these tyrosine-phosphorylated proteins was observed only in the Triton X-100-insoluble fraction, suggesting that these proteins could be associated with the cytoskeleton. Increased tyrosine phosphorylation of these proteins upon E. coli infection was observed in both transformed and primary human renal epithelial cells and in cells infected with several different strains of E. coli isolated from the feces of healthy individuals or from the blood or urine of patients with pyelonephritis. The increased tyrosine phosphorylation of these proteins required live bacteria and was blocked by tyrosine kinase inhibition but not by protein synthesis inhibitors or cytochalasin D. These experiments establish a strong link between E. coli internalization and host cell signaling through tyrosine kinases in human kidney cells and provide evidence that specific proteins are involved in these processes.
Triaxial Burke-Schumann Flames with Applications to Flame Synthesis (2000)

The problem of a flame generated by three coaxial flows is solved by extending the Burke-Schumann methodology to include a third stream. The solution is particularly relevant to flame synthesis wherein multiple tubes are often employed either to introduce inert as a diffusion barrier or to introduce more than two reactants. The general problem is solved where the inner and outer tubes contain reactants and the middle tube contains either an inert or a third reactant. Relevant examples are considered and the results show that the triaxial Burke-Schumann flame can be substantially more complicated than the traditional Burke-Schumann flame. When the middle flow is inert the flame temperature is no longer constant but increases axially, reaching a maximum at the flame centerline. At the exit the flame does not sit on the tube exit but instead resides between the inner and outer tubes, resulting in an effective barrier for particle build-up on the burner rim. For the case of a third reactant in the middle flow, synthesis chemistry where the inner reaction is endothermic and the outer reaction is exothermic is considered. In addition to showing the flame temperature and flame shape, the results identify conditions wherein reaction is not possible due to insufficient heat transfer from the outer flame to support the inner flame reaction.
Best Practices for Using AI When Writing Scientific Manuscripts (2023)

TEST 02 - Elsevier's Scopus, the largest abstract and citation database of peer-reviewed literature. Search and access research from the science, technology, medicine, social sciences and arts and humanities fields.
Effect of surface interactions on the hysteresis of capillary condensation in nanopores (2007)

Gas adsorption and liquid desorption of a number of organic vapors in anodized nanoporous alumina, with controlled geometry (cylindrical pore diameters from 10 to 60 nm), are studied using optical interferometry. The narrow-diameter distribution of disconnected pores allows checking the validity of the (long-predicted but not experimentally verified) Kelvin equation without any adjustable parameters, modeling or other assumptions. Evaporation occurs at liquid-vapor equilibrium according to this equation, whereas condensation occurs from metastable states of the vapor phase by nucleation, enhanced by surface defects inside the nanopores. This produces hysteresis, in qualitative agreement with theoretical models and simulations that use Van der Waals interactions between the fluid and the pore surface. The reproducibility of the hysteresis depends on the strength of these interactions, which play an important role in the dynamics of capillary condensation.
3D printing of round microfluidic channels to mimic the microvasculature (2018)

Not available.
Long-Term Outcomes of Smile Reconstruction in Möbius Syndrome (2018)

Segmental gracilis muscle transplantation provides long-lasting, durable improvements in objective and patient-reported measures of facial function.
Reaktionen mit tert.‐Butoxyradikalen. V. Der Deuteriumisotopeneffekt bei der Reaktion von tert.‐Butoxyradikalen mit aliphatischen Ketonen (1965)

Abstract Die Reaktivität von Aceton, Diäthylketon, Cyclohexanon und der entsprechenden α‐deuterierten Ketone bei der H‐Abspaltung durch tert.‐Butoxyradikale wird bestimmt. Die α‐CH 2 ‐Gruppen des Diäthylketons sind 13mal reaktiver als die β‐CH 3 ‐Gruppen; die Reaktionsfähigkeit der beiden α‐CH 2 ‐Gruppen des Cyclohexanons ist 10mal größer als die der drei β‐und γ‐CH 2 ‐Gruppen. Der Deuteriumisotopeneffekt bei der H‐Abspaltung durch tert.‐Butoxyradikale aus der α‐Stellung beträgt 5,4 bei Aceton, 3,9 bei Diäthylketon und 1,4 bei Cyclohexanon.
Accurate Control of a Pan-tilt System Based on Parameterization of Rotational Motion (2018)

A pan-tilt camera system has been adopted by a variety of fields since it can cover a wide range of region compared to a single fixated camera setup. Yet many studies rely on factory-assembled and calibrated platforms and assume an ideal rotation where rotation axes are perfectly aligned with the optical axis of the local camera. However, in a user-created setup where a pan-tilting mechanism is arbitrarily assembled, the kinematic configurations may be inaccurate or unknown, violating ideal rotation. These discrepancies in the model with the real physics result in erroneous servo manipulation of the pan-tilting system. In this paper, we propose an accurate control mechanism for arbitrarily-assembled pan-tilt camera systems. The proposed method formulates pan-tilt rotations as motion along great circle trajectories and calibrates its model parameters, such as positions and vectors of rotation axes, in 3D space. Then, one can accurately servo pan-tilt rotations with pose estimation from inverse kinematics of their transformation. The comparative experiment demonstrates out-performance of the proposed method, in terms of accurately localizing target points in world coordinates, after being rotated from their captured camera frames.
Abstract 155: MicroRNA ‘signature’ during estrogen-mediated mammary carcinogenesis (2011)

Abstract MicroRNAs (miRNAs) play important role in a wide range of normal biological processes. Dysregulated miRNA expression has been reported with the development and progression of cancers, including breast cancer. The role of estrogens in regulation of cell proliferation and breast carcinogenesis is well known. Recent reports have associated several miRNAs with estrogen receptors in breast tumors. However, identification of miRNA ‘signature’ during estrogen-mediated carcinogenesis may be critical for understanding the effect of estrogen in human breast cancer, as well as for developing strategies for cancer chemoprevention. In this study we analyzed miRNAs at 3 weeks, 12 weeks close to the appearance of first palpable tumor and 28 weeks when tumor incidence reaches 85-90% following treatment of female ACI rats with 17ß-estradiol (E2) silastic implants. Total RNA from mammary tissues and tumors were isolated using mirVana microRNA kit and analyzed using Affymetrix GeneChip miRNAarray. This array had 351 rat specific miRNAs probes. E2 treatment resulted in significant up regulation or repression (2 fold change) of 33 miRNAs at two or more time points. A total of 9 (p&amp;lt;0.00128), 8 (p&amp;lt;0.00113) and 27 (p&amp;lt;0.00384) miRNAs in E2 treated normal mammary tissue, and 73 (p&amp;lt;0.01039) in tumor tissue were differentially expressed when compared to untreated samples from 3, 12, and 28 weeks time point, respectively, with false discovery rate (FDR) at 0.05. These miRNAs were used to predict potential target mRNAs using Partek software. Ingenuity pathway analysis was used to further predict the major functions of the target mRNAs and molecular pathways. Molecular mechanism of cancer was the top canonical pathway of putative target mRNAs at all the time points indicating influence of E2 treatment in mammary carcinogenesis. The five top-ranking miRNAs (miR-196c, miR-34c, miR-375, miR-429 and miR-206) from microarray analysis were confirmed by real-time RT-PCR using Taqman microRNA assays; 5S RNA was used as reference for normalization. The expression of these miRNAs followed the same pattern whether evaluated by microarray or qPCR. These five miRNAs were further predicted to interact with mRNAs involved in estrogen-mediated (e.g. Src, PI3K, Akt, etc.) and EGF-mediated (e.g. Sos, Ras, MEK, etc.) breast cancer pathways. Thus, our results demonstrate miRNA modulations during the estrogen-mediated mammary carcinogenesis in an in vivo model in which, histologically, the mammary tumors resemble human breast cancer. Our future studies are aimed at finding the effect of naturally-occurring anti-cancer agent(s) on these candidate miRNAs to determine their translational importance (Supported by CA-118114, CA-125152 and Agnes Brown Duggan Endowment). Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 155. doi:10.1158/1538-7445.AM2011-155
Adenosine A2A Receptor Antagonists: New 8‐Substituted 9‐Ethyladenines as Tools for in vivo Rat Models of Parkinson's Disease (2009)

A new series of 8-substituted 9-ethyladenine derivatives has been synthesized and tested at rat and human adenosine receptors. Binding data demonstrates that some compounds could represent new tools suitable for in vivo studies in rat models of Parkinson's disease and for the design of new molecules with improved affinity and selectivity at human AA(2A)R.Clinical evidence has demonstrated that AA(2A)R antagonists could be an alternative approach to the treatment of Parkinson's disease. Recently, three 9-ethyladenine derivatives bearing a bromine atom, an ethoxy group, and a furyl ring, respectively, in the 8-position have been reported to ameliorate motor deficits in rat Parkinson's disease models, suggesting a potential therapeutic role for these compounds. Starting from these observations, a new series of 9-ethyladenine derivatives, bearing different substituents such as halogens, alkoxy groups, aromatic and heteroaromatic rings in the 8-position, were synthesized. Radioligand binding assays demonstrated that some of the new compounds bind rat AA(2A)R with higher affinity than the previously reported congeners and that there is a good correlation between binding to rat and human receptors. Hence, the new molecules could represent new tools suitable for the in vivo studies in rat models of Parkinson's disease. Finally, a molecular docking analysis of the compounds was performed using a homology model of rat AA(2A)R, built using the human crystal structure as the template, and results are in agreement with the binding data.