gebhart/patrepeval huggingface dataset:
Niclosamide exerts anti-tumor activity through generation of reactive oxygen species and by suppression of Wnt/ β-catenin signaling axis in HGC-27, MKN-74 human gastric cancer cells (2020)

Introduction Gastric carcinoma (GC) remains a therapeutic challenge despite having many potent drugs to treat. Various studies emphasized the role of dysregulated Wnt/β-catenin pathway in cancer. In the present study, we examined the anti-cancer effect of Niclosamide and its effect on the dysregulated β-catenin pathway in human gastric carcinoma cell lines. Methods Cytotoxicity of compound to gastric cancer cell line was assessed by MTT cell viability assay, cell cycle analysis, and apoptosis assay was done using standard kits of Muse™ Cell Analyser. Reactive oxygen species (ROS) generation and mitochondrial membrane potential were analyzed by 2′,7′-Dichlorodihydrofluorescein diacetate (DCFDA) and tetraethylbenzimidazolylcarbocyanine iodide (JC-1) staining respectively. Protein expression studies were carried out by standard western blotting protocols. Results Niclosamide treatment resulted in a dose-dependent inhibition of viability of the gastric carcinoma cell-lines induced cell cycle arrest in the G0/G1 phase and strongly induced apoptosis in a concentration-dependent manner by downregulating Cyclin-D1 and CDK4 levels, critical proteins required for G1-S phase progression. DCFDA and JC-1 staining results indicated that Niclosamide enhanced intracellular ROS generation and disrupted mitochondrial membrane potential. Furthermore, niclosamide treatment decreased the expression of NF-KB, Bcl-2 and increased the expression of Bax protein. Niclosamide treatment significantly decreased the β-catenin mediated transcriptional activity and down-regulated β-catenin levels and its downstream proteins cyclinD1, CDK-4, and c-myc expression and also impeded Akt phosphorylation, a common internode in the Wnt and Akt/mTOR signaling in HGC-27 cells. Conclusion This study demonstrated that Niclosamide might become a promising therapeutic agent for the management of gastric cancer and further warrants its clinical trials in gastric cancer patients.
A fast analog controller for a unity-power factor AC/DC converter (2002)

This paper presents an analog implementation of a fast controller for a unity-power-factor (UPF) AC/DC converter. Unlike low bandwidth controllers associated with many state-of-the-art UPF converters this fast controller can exercise control action at a rate comparable to the switching frequency rather than the line frequency. In order to accomplish this while maintaining unity power factor, the fast controller employs a ripple-feedback cancellation scheme. In addition to responding faster to disturbances, the controller facilitates the replacement of bulky bus capacitors with smaller, less expensive alternatives.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Sol-gel coated glass cells for spin-exchange polarized He3 (2000)

We have developed a high-purity sol-gel coating for the interior surface of glass cells used for polarizing He3 by spin-exchange optical pumping. The coating is designed to minimize spin relaxation due to wall collisions. A longitudinal spin-relaxation time T1 in a sol-gel coated Pyrex cell of 344±8 h was achieved, the longest T1 we have ever recorded for a gaseous sample. Repeated trials indicated that the coating was quite robust. Results using an uncoated Pyrex cell were also quite good, although inferior to the performance of the coated cell.
Kinetic and Thermodynamic Insights on Agonist Interactions with the Parathyroid Hormone Receptor-1 from a New NanoBRET assay (2022)

Abstract Polypeptides that activate the parathyroid hormone receptor-1 (PTHR1) are important in human physiology and medicine. Most previous studies of peptide binding to this receptor have involved displacement of a radiolabeled ligand. We report a new assay format based on bioluminescence resonance energy transfer (BRET). Fusion of a nanoluciferase (nLuc) unit to the N-terminus of the PTHR1 allows direct detection of binding by an agonist peptide bearing a tetramethylrhodamine (TMR) unit. Affinity measurements from the BRET assay align well with results previously obtained via radioligand displacement. The BRET assay offers substantial operational benefits relative to affinity measurements involving radioactive compounds. The convenience of the new assay allowed us to explore several questions raised by earlier reports. For example, we show that although the first two residues of PTH(1-34) (the drug teriparatide) are critical for PTHR1 activation, these two residues contribute little or nothing to affinity. Comparisons among the well-studied agonists PTH(1-34), PTHrP(1-34) and “long-acting PTH” (LA-PTH) reveal that the high affinity of LA-PTH arises largely from a diminished rate constant for dissociation relative to the other two.
Multi-site near-infrared tomographic imaging of the brain (2004)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text D. P. Klemer, R. L. Barbour, C. H. Schmitz, H. L. Graber, Y. Pei, R. E. Hardin, M. S. Katz, N. A. Franco, and A. G. Smeraldi, "Multi-site near-infrared tomographic imaging of the brain," in Biomedical Topical Meeting, OSA Technical Digest (Optica Publishing Group, 2004), paper WF35. Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
Integrated transition of coplanar to rectangular waveguides (2001)

Usual transitions between planar circuit and rectangular waveguide make use of 3-D complex mounting structures. Such an integration requires costly high precision mechanical alignment, In this paper, a new planar platform is developed in which a coplanar waveguide (CPW) and a rectangular waveguide are fully integrated on the same substrate, and they are interconnected via a simple transition. They can be built with a standard PCB process. Our experiments at 28 GHz show that an effective bandwidth of 7% at 15 dB return loss can easily be achieved. The CPW-to-waveguide transition allows for a complete integration of waveguide components on substrate with active components such as MMIC.
ChemInform Abstract: A Benzoquinone Diimine from the Oxidation of Pentafluoroaniline by Hypochlorite. X‐Ray Crystal Structure and Possible Formation via Pentafluorophenylnitrene. (1989)

Abstract In addition to the decafluoroazobenzene (III) and the phenazine (IV), a dark red solid is observed which is identified as benzoquinone diimine (V) (space group: P1, Z=4).
Synthesis, fabrication and characterization of a highly-dispersive mirrors for the 2 µm spectral range (2017)

We report a challenging design, fabrication and post-production characterization problem of a dispersive mirror supporting the spectral range from 2000 nm to 2200 nm and providing a group delay dispersion of -1000 fs<sup>2</sup>. The absolute reflectance in the working range is over 99.95%. The reported mirror is a critical element for Tm and Ho based lasers and paves the way for the development of ultrafast 2 µm lasers with sub-100 fs pulse duration.
Reflections on Parmigianino's Self portrait in a convex mirror : a computer graphics reconstruction of the artist's studio (2010)

We built a full computer graphics model of Parmigianino's studio, including convex mirror, in order to explore the artist's likely working methods during his execution of <i>Self portrait in a convex mirror</i> (1523-4). Our model supports Vasari's record that the radius of curvature of a convex mirror matched the radius of curvature of the wood panel support. We find that the image in the painting is consistent with a simple horizontal rectilinear room drawn from a slightly re-oriented and re-positioned mirror. Our optical analyses lead us to recommend an alteration to the current display arrangement in the Kunsthistorisches Museum.
Major depression in a large family in Pakistan : no relationship to inbreeding, economic status or rural living. (2012)

Objective: To describe the relationships between depression and gender, socio-economic status, education, urban or rural living and coefficient of inbreeding in a well defined relatively homogeneous population. &#13;\n&#13;\nDesign: Descriptive study.&#13;\n&#13;\nPlace and Duration of Study: King Edward Medical College, Lahore from January 1999 to January 2002.&#13;\n&#13;\nSubject and Methods: Subjects were interviewed and diagnosed by a trained psychiatrist and information was collected on various variables. Inbreeding coefficients were calculated from genealogical data.&#13;\n&#13;\nResults: Of 211 persons interviewed, 182 had a lifetime diagnosis of recurrent major depression and 26 had no psychiatric diagnosis. Multiple regression analysis revealed greater severity and earlier age at onset in women than men but no significant correlation of severity or age at onset with socioeconomic status, urban versus rural living or coefficient of inbreeding.&#13;\n&#13;\nConclusion: This appears to be a genetically homogenous sub-type of depression and further genetic studies may reveal underlying genetic risk factors.
Highlighting Diverse Concepts in Documents (2009)

We show the underpinnings of a method for summarizing documents: it ingests a document and automatically highlights a small set of sentences that are expected to cover the different aspects of the document. The sentences are picked using simple coverage and orthogonality criteria. We describe a novel combinatorial formulation that captures exactly the document-summarization problem, and we develop simple and efficient algorithms for solving it. We compare our algorithms with many popular document-summarization techniques via a broad set of experiments on real data. The results demonstrate that our algorithms work well in practice and give high-quality summaries.
Aqueous transfer of colloidal metal oxide nanocrystals via base-driven ligand exchange (2022)

A general method is developed for removal of native nonpolar oleate ligands from colloidal metal oxide nanocrystals of varying morphologies and compositions. Ligand stripping occurs by phase transfer into potassium hydroxide solution, yielding stable aqueous dispersions with little nanocrystal aggregation and without significant changes to the nanomaterials' physical or chemical properties. This method enables facile fabrication of conductive films of ligand-free nanocrystals.
True 3D display and BeoWulf connectivity (2003)

We propose a novel true 3-D display based on holographic optics, called HAD (<i>Holographic Autostereoscopic Display</i>), or <i>Holographic Inverse Look-around and Autostereoscopic Reality </i>(HILAR), its latest generation. It does not require goggles, unlike the state of the art 3-D system which do not work without goggles, and has a table-like 360&deg; look-around capability. Also, novel 3-D image-rendering software, based on Beowulf PC cluster hardware is discussed.
Giotto und die Erfindung der Perspektive - Ein „medienphilosophisches“ Phänomen (2011)

In der Renaissance ist die Erfindung der Perspektive ein Meilenstein auf dem Weg der künstlerischen und später der naturwissenschaftlichen Erfassung der räumlichen Welt mittels einer Systematisierung von Größen zueinander. Die Anfänge der perspektivischen Darstellung liegen bereits in der Antike begründet. Wenn auch „entsprechend der Tiefendistanz [...] [und] Konvergenz paralleler Linien […] [eine] gewisse Vorstellung von Raumtiefe“ erzeugt werden konnte, blieb den antiken Künstlern eine „ges...
High frequency plant proliferation via direct fronds regeneration of Korean endemic duckweed species (2009)

High frequency plant proliferation system via direct frond regeneration of endemic duckweed plants Lemna paucicostata and Spirodela polyrhiza was established. Fronds of L. paucicostata and S. polyrhiza were able to multiply half-strength MS basal medium without plant growth regulators. However, addition of BA at a range of 1 to 3 mg/L was more effective than high concentration of BA treatments for fronds proliferation. Also half-strength MS salts was suitable for the fronds proliferation. Increase of salts concentration had inhibitory effect on fronds proliferation. Also the frequency of callus formation from fronds of L. paucicostata was 3.3%, when they cultured onto 1/2 MS medium supplemented with 1 mg/L of BA. Similarly the frequency of callus formation from S. polyrhiza was very low. After subculture of white globular structures derived from fronds of L. paucicostata, numerous globular somatic embryos and calluses were developed onto the surface of fronds. However these somatic embryos did not fully develop into normal plants when transferred to 1/2 MS basal medium. Therefore direct frond regeneration system was more efficient for mass proliferation of L. paucicostata and S. polyrhiza. The plant regeneration system of L. paucicostata and S. polyrhiza established in this study, might be applied to mass proliferation and genetic transformation for molecular breeding.
NeoLISA - A new method of surfactant administration developed based on in situ simulation&nbsp; (2020)

Abstract Background: Respiratory distress syndrome (RDS) caused by surfactant deficiency is the major cause of respiratory distress in preterm neonates. Despite the advances in preventive interventions, RDS may still develop and be associated with acute and or chronic complications. Exogenous surfactant therapy is effective in reducing RDS related mortality and morbidity. Due to the complications of surfactant administration, minimal or less invasive administration techniques have been developed and appear promising. Objective: To address the knowledge gaps in less invasive surfactant administration technique at the level of the health care worker (HCW). Methodology: Four simulation-training sessions were conducted in April 2020, two before and two after the NeoLISA technique was developed. The neonatal clinical team consisted of neonatology specialists and neonatal nurses. Results: Each simulation session lasted for around 30 minutes including debriefing. The learnings during this process were discussed and the new technique of NeoLISA was developed. The key change was the development to confirm the catheter position. Conclusion: A new method for surfactant administration was developed. An innovative bedside clinical method confirming catheter position into the trachea has been developed.
Dose-dependent role of the cohesin complex in normal and malignant hematopoiesis (2015)

Normal hematopoiesis is orchestrated by master regulatory transcription factors that are expressed in specific cell types at discrete differentiation stages (Novershtern et al., 2011;Lara-Astiaso et al., 2014).Tight coordination of these networks maintains the balance between hematopoietic stem/progenitor cell (HSPC) self-renewal and lineage commitment.In contrast, acute leukemias are characterized by increased self-renewal and impaired differentiation, often in the setting of mutations in genes with a known or postulated role in regulating transcriptional output (Dawson et al., 2012).Recent cancer genome sequencing studies have identified loss of function mutations in cohesin complex factors among patients with solid tumors (Solomon et al., 2011;
Stringent and Relaxed Specificities of TaqI Endonuclease: Interactions with Metal Cofactors and DNA Sequences (1995)

We have studied the roles of metal cofactors Mg2+ and Mn2+ in modulating substrate specificities during the enzymatic cycle of TaqI endonuclease using steady state and single-turnover kinetics. In the presence of Mg2+, stringent discrimination of TaqI against single base-pair changes (star sites) is manifested by the loss of tight, specific binding in the early stage of the enzymatic cycle. In the presence of Mn2+, relaxed specificity for a star site sequence is attributed to formation of three distinct classes of the ternary complexes: the highly activated TaqI-cognate-Mn2+ complex; the partially activated TaqI-star-Mn2+ complex; and the ground state, inactive TaqI-nonspecific-Mn2+ complex. In addition to a high affinity for a TaqI-DNA complex, Mn2+ also binds to TaqI in a DNA-independent fashion. This may facilitate enzyme activation, which could account for the observed relaxation in substrate specificity. Thus, the TaqI-DNA-Mn2+ complex could be formed by either of two pathways: TaqI binding to DNA followed by the binding of Mn2+ or TaqI first binding to Mn2+ followed by the addition of DNA. The inactive, nonspecific TaqI-star-Mg2+ complex virtually prohibits transition state interactions, but a TaqI-star-Mn2+ complex attains a measurable single-turnover rate. In the late stages of the enzymatic cycle, high affinity of Mn2+ to a TaqI-DNA complex and to the TaqI enzyme may also account for a slower rate of product release.
The Safety and Efficacy of Direct Trocar Insertion With Elevation of the Rectus Sheath Instead of the Skin for Pneumoperitoneum (2005)

No AccessJournal of UrologyUrological Survey: Urolithiasis/Endourology: Urolithiasis, Endourology and Laparoscopy1 Nov 2005The Safety and Efficacy of Direct Trocar Insertion With Elevation of the Rectus Sheath Instead of the Skin for Pneumoperitoneum Ralph V. ClaymanM.D. Ralph V. ClaymanRalph V. Clayman More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)68807-4AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "The Safety and Efficacy of Direct Trocar Insertion With Elevation of the Rectus Sheath Instead of the Skin for Pneumoperitoneum." The Journal of Urology, 174(5), pp. 1847–1848 © 2005 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 174Issue 5November 2005Page: 1847-1848 Advertisement Copyright & Permissions© 2005 by American Urological Association, Inc.MetricsAuthor Information Ralph V. Clayman More articles by this author Expand All Advertisement Loading ...
Measurement-Based Validation of Z3RO Precoder to Prevent Nonlinear Amplifier Distortion in Massive MIMO Systems (2022)

In multiple input multiple output (MIMO) systems, precoding allows the base station to spatially focus and multiplex signals towards each user. However, distortion introduced by power amplifier nonlinearities coherently combines in the same spatial directions when using a conventional precoder such as maximum ratio transmission (MRT). This can strongly limit the user performance and moreover create unauthorized out-of-band (OOB) emissions. In order to overcome this problem, the zero third-order distortion (Z3RO) precoder was recently introduced. This precoder constraints the third-order distortion at the user location to be zero. In this work, the performance of the Z3RO precoder is validated based on real-world channel measurement data. The results illustrate the reduction in distortion power at the UE locations: an average distortion reduction of 6.03 dB in the worst-case single-user scenario and 3.54 dB in the 2-user case at a back-off rate of -3 dB.
Surface Wettability Prediction Using Image Analysis and an Artificial Neural Network (2022)

In this study, a wettability-predicting method that uses an artificial neural network (ANN) by learning from digital images of the actual surface structures was developed. Polyester film surfaces were treated with oxygen plasma to realize various nanostructured surfaces. Surface structural characteristics from SEM images were quantified in a multifaceted way using a box-counting algorithm, a gray-level co-occurrence matrix algorithm, and binary image analysis. An ANN model that can predict wettability from surface structures was developed using the quantified surface structure and the resulting wettability as learning data. Furthermore, a surface with an optimal nanostructure to achieve superhydrophobicity was suggested by considering extracted surface structural parameters that significantly affect the surface wettability.
Polymerization Efficacy Affects the Long-term Mini-interfacial Fracture Toughness. (2017)

A one-step self-etch adhesive should contain photo-initiator in a sufficiently high concentration, as this directly improves its immediate and long-term bonding efficacy to dentin. While the mini-iFT remained stable up to 6 month of aging for both the commercial and the adhesive with a high concentration of photo-initiator, the mini-iFT was found to decrease after 1 year of aging.
Imaging Tumor Burden in the Brain with 89Zr-Transferrin (2012)

The results from this study suggest that (89)Zr-transferrin could be a broadly applicable tool for identifying and monitoring tumors in the brain, with realistic potential for near-term clinical translation.
Understanding How the Antimicrobial Peptide Thanatin Interacts with the Lipid Bilayer of Cell Walls Using Model Membranes (2014)

453-Pos Board B208 The Interaction Between the Antimicrobial Peptide K-Hya1 and Model Membranes: Distinct Action in Neutral or Negatively Charged Bilayers Thais A. Enoki1, Karin A. Riske2, Katia R. Perez2, Esteban N. Lorenzon3, Eduardo M. Cilli3, M. Teresa Lamy1. Institute of Physics, Sao Paulo University, Sao Paulo, Brazil, Department of Biophysics, Federal University of Sao Paulo, Sao Paulo, Brazil, Institute of Physics, Sao Paulo State University, Sao Paulo, Brazil. Antimicrobial peptides (AMPs) are important molecules for the innate immune defense system of plants and animals. The antimicrobial peptide Hylina1 (Hya1) was isolated from arboreal South African frog Hypsiboas albopunctatus. In this work, we investigate the interaction of an analogue peptide, KHya1 (KIFGAIWPLALGALKNLIK-NH2) with model membranes composed of DPPC (dipalmitoyl phosphatidylcholine), DPPG dipalmitoyl phosphatidylglycerol) and DPPC:DPPG 1:1 or of POPC (palmitoyl oleoyl phosphatidylcholine) and POPC:POPG (palmitoyl oleoyl phosphatidylglycerol) with different techniques. Differential Scanning Calorimetry (DSC) profiles show distinct environments in the bilayer with anionic lipids, suggesting a disturbed region due to peptide adsorption, and an unaltered region. On the other hand, in neutral membranes an average perturbation is observed, which suggests a superficial interaction. Steady-state fluorescence spectroscopy of the intrinsic Trp residue shows a deeper insertion of this residue into charged bilayers as compared with neutral membranes. Dye-leakage experiments show that membrane charge also modulates the kinetics of membrane permeabilization, which is much faster for charged bilayers. Optical microscopy of giant unilamellar vesicles (GUVs) in the fluid phase revealed a different mechanism of action of the peptide in the presence or absence of negatively charged lipids. K-Hya1 induces small perturbations in POPC vesicles, causing membrane permeabilization without morphological changes. On the other hand, the peptide induces permeabilization accompanied by a large increase in surface area of POPC:POPG vesicles, suggesting the opening of several pores in the bilayer. Taken together, the results clearly show a peptide-bilayer interaction modulated by the presence of negatively charged lipids. Data can be interpreted as a different orientation of the peptide in the bilayer: parallel to the surface in neutral membranes and stable and crossed in anionic membranes.
Thermal Stress-Aware CMOS–SRAM Partitioning in Sequential 3-D Technology (2020)

This article explores the feasibility of high-temperature annealing for top-tier devices in a sequential 3-D (Seq3D) technology. Thermally stable bottom-tier device and interconnect design guidelines are provided. CMOS-SRAM partitioning is proposed to achieve performance gain from Seq3-D. The implications of thermally stable Seq3-D on system-level performance are evaluated. Seq3-D wafer and die cost of ownership are estimated.