gebhart/patrepeval huggingface dataset:
Isolation of poly (A)+ mRNA for downstream reactions from some medicinal and aromatic plants. (2005)

In the present protocol for extraction of RNA, hexadecyltrimethylammoniumbromide (CTAB) and insoluble polyvinylpyrrolidone were used followed by LiCl precipitation, CsCl ultracentrifugation and finally poly (A)+ mRNA was isolated with the help of oligo(dT)-cellulose columns. The isolated poly (A)+ mRNA was found to be suitable for cDNA-AFLP and suppression subtractive hybridization applications. It is a modified and consolidated protocol based on previously described methods for isolated steps and works better for medicinal and aromatic plants. High yield of poly (A)+ mRNA coupled with its amenability for downstream reactions like RT-PCR, northern blotting and cDNA synthesis for library construction is a key feature of the present protocol.
Design and Validation of an In-Mold Shrinkage Sensor (2008)

Dimensional consistency is a critical attribute of molded products, yet part dimensions are frequently only estimated from cavity pressure or part weight measurements. An in-mold shrinkage sensor is designed and validated. The design includes a deflectable diaphragm instrumented with strain gages connected in a full bridge circuit. In operation, the diaphragm is deflected due to the pressure of the melt in the mold cavity. Molded part shrinkage is then measured as the polymer melt solidifies, shrinks, and retracts from the mold wall. A design of experiments is conducted to validate the performance of the sensor as a function of packing pressures, cooling time, melt temperature, and coolant temperature. The results indicate the sensor outperforms both cavity pressure transducers and regression models, and is able to measure the shrinkage to an absolute accuracy of 0.01 mm for a 2.5 mm thick part.
Cx3cr1-deficient microglia exhibit a premature aging transcriptome (2019)

CX3CR1, one of the highest expressed genes in microglia in mice and humans, is implicated in numerous microglial functions. However, the molecular mechanisms underlying <i>Cx3cr1</i> signaling are not well understood. Here, we analyzed transcriptomes of <i>Cx3cr1-</i>deficient microglia under varying conditions by RNA-sequencing (RNA-seq). In 2-mo-old mice, <i>Cx3cr1</i> deletion resulted in the down-regulation of a subset of immune-related genes, without substantial epigenetic changes in markers of active chromatin. Surprisingly, <i>Cx3cr1-</i>deficient microglia from young mice exhibited a transcriptome consistent with that of aged <i>Cx3cr1-</i>sufficient animals, suggesting a premature aging transcriptomic signature. Immunohistochemical analysis of microglia in young and aged mice revealed that loss of <i>Cx3cr1</i> modulates microglial morphology in a comparable fashion. Our results suggest that CX3CR1 may regulate microglial function in part by modulating the expression levels of a subset of inflammatory genes during chronological aging, making <i>Cx3cr1</i>-deficient mice useful for studying aged microglia.
Enzyme-linked immunosorbent assay for identification and measurement of antibodies to group A streptococcal bacteriophage. (1985)

A sensitive enzyme immunoassay (ELISA) was developed to identify and measure antibodies to group A streptococcal bacteriophage hyaluronidase. With a purified preparation of bacteriophage hyaluronidase as the solid-phase antigen, the ELISA was shown to be as specific as and more sensitive than the standard bacteriophage neutralization test for measurement of antibody to bacteriophage. In rabbits immunized with bacteriophage, the ELISA detected antibody earlier than the neutralization assay (7 vs. 11 days) and was able to distinguish IgG and IgM class antibodies. A strong correlation was demonstrated between antibody titers measured by ELISA and bacteriophage neutralization (r = 0.88; P less than 0.001). Preliminary data using the ELISA, modified to measure human antibody to bacteriophage hyaluronidase, indicated that an antibody response of both IgG and IgM classes occurred in humans after group A streptococcal infection. This ELISA provided a sensitive method for detection and measurement of antibody to a specific bacteriophage antigen, which will be useful in the investigation of the role of bacteriophage in the pathogenesis of group A streptococcal infections.
The Los Angeles Freeway Service Patrol (FSP) Evaluation: Site Selection and Database Development (1997)

The Los Angeles Freeway Service Patrol (FSP) Evaluation project is measuring the effectiveness of the FSP program on a specific freeway site in Los Angeles. This report describes the site selection and database development phases of the project. From an initial list of ten possible sites, detailed analysis was performed in order to rank the sites according to specific parameters developed by the study team. Site selection was based on congestion levels, average travel speeds, shoulder width, number of in-lane FSP assists, average daily traffic, directionality and the density of functional loop detectors. The site selected was FSP Beat 8, which is located on Interstate Route 10, between Eastern Avenue and Santa Anita Avenue.Once the site was selected, a detailed, comprehensive, computerized database was developed. This database completely describes the traffic conditions along Beat 8 for 32 weekdays, for a total of six hours each day. This 192-hour database includes detailed descriptions for 1,560 incidents, tach vehicle travel time traces for 3,619 runs (at 5.7 minute headways), and loop detector data (30-second flow and occupancy) from 240 loop detectors. Finally, the CHP has provided electronic CAD logs for the entire study period. Further to the documentation included in this report, preliminary incident analysis is included in PATH Working Paper 97-18 and the methodology and preliminary evaluation results are included in PATH Working Paper 97-17.
Incorporating specific absorption rate constraints into wireless signal design (2014)

Portable wireless devices used in close proximity to the human body have transmitter power constraints that are dictated in part by regulatory limits on a form of electromagnetic exposure called specific absorption rate (SAR). SAR, measured in Watts per kilogram, is a measure of electromagnetic energy absorption by the body, which can cause the heating of tissue. Some portable wireless devices sold today operate near the accepted human SAR limit. This article examines how SAR constraints can be incorporated into wireless signal design for multiple transmitters. A SAR constraint, although related to a power constraint, differs sufficiently that signals designed specifically for the constraint are needed. Although it is well known that multiple transmitters subject only to a power constraint can improve the link performance of a wireless system compared to a single transmitter, it is not clear when the device is also subject to a SAR constraint: how to model the constraint; whether performance advantages still exist; and how to realize any advantages. In this article, we introduce SAR constraints and discuss the SAR measurement, simulation, and modeling process. We then show how to incorporate the SAR constraint into system performance analysis and code design. We show how SAR codes use multiple transmit antennas to get good combined farfield error performance and near-field SAR performance, improving handset transmitter performance in the critical uplink of a communication system.
Cognitive illusions and the lying machine: a blueprint for sophistic mendacity (2010)

This dissertation concerns the development of a prototype lying machine—a mechanical sophist whose disingenuous rhetoric seduces humans into believing falsehoods. The development approach is to empower a machine with a predictive psychological theory of human reasoning (specifically, Philip N. Johnson-Laird's mental models theory) and to use this theory as part of the machine's computational theory of mind, i.e., its ascriptive theory about the mental contents and operations of its audience. In operation the machine uses mental models theory to exploit cognitive illusions, highly fallible heuristics and biases in human reasoning; the machine incorporates cognitive illusions into its arguments, which are thus called illusory arguments. Since the machine articulates illusory arguments with the intent to deceive its audience, the machine is a liar and a teller of sophistic lies. 
The dissertation lays out the philosophical, psychological, and computational foundations for this lying machine, and provides empirical evidence that the machine reliably deceives ordinary humans. It presents the philosophic rationale for the claim that the machine lies, or at least simulates lying, and includes description of sophistic lies and sufficient conditions for lying sophistically (the form of lying enacted by the machine). Additionally, the work contains a formal specification of a mental models theory for propositional reasoning, and theoretically and computationally extends this theory to the generation of credible arguments. Also described are two psychological experiments that test the illusory and persuasive effect of the machine's sophisms. The results of these experiments confirm that the machine is a potent liar, and validate the approach to mechanizing sophistic deception.
The described research builds on and is contrasted with contemporary philosophic accounts of lying, cognitive and psychological theories of human reasoning, and the thematic and theoretic pursuit of persuasive technologies and computational models of natural argument. This work is inspired by and linked to philosophy of mind and language, artificial intelligence, computational cognitive modeling, argumentation theory, fallacy theory, and classical Greek rhetoric.
Time multiplexing for increased FOV and resolution in virtual reality (2017)

We introduce a time multiplexing strategy to increase the total pixel count of the virtual image seen in a VR headset. This translates into an improvement of the pixel density or the Field of View FOV (or both) A given virtual image is displayed by generating a succession of partial real images, each representing part of the virtual image and together representing the virtual image. Each partial real image uses the full set of physical pixels available in the display. The partial real images are successively formed and combine spatially and temporally to form a virtual image viewable from the eye position. Partial real images are imaged through different optical channels depending of its time slot. Shutters or other schemes are used to avoid that a partial real image be imaged through the wrong optical channels or at the wrong time slot. This time multiplexing strategy needs real images be shown at high frame rates (&gt;120fps). Available display and shutters technologies are discussed. Several optical designs for achieving this time multiplexing scheme in a compact format are shown. This time multiplexing scheme allows increasing the resolution/FOV of the virtual image not only by increasing the physical pixel density but also by decreasing the pixels switching time, a feature that may be simpler to achieve in certain circumstances.
Epidermis-specific gene expression in Pachyphytum. (1992)

Transcripts of exclusively epidermis-specific expression were obtained by differential screening of a cDNA library prepared from isolated epidermis tissue of a succulent plant, Pachyphytum. Six transcripts were selected and characterized by RNA gel blot hybridization. Five cDNAs represented abundant mRNAs found exclusively in the epidermis. In situ hybridizations with three of these transcripts further substantiated their epidermal location. While two transcripts were present in all cells of the epidermis, one transcript was enriched in subsidiary cells of the stomatal complexes. All of the transcripts were highly expressed in the protoderm of the shoot apical meristem. DNA sequence determination indicated that the mRNAs identified represent novel sequences, encoding yet unknown plant functions. These transcripts, their control elements, and their encoded functions should help to advance our understanding of epidermal cell determination and differentiation.
[Dynamic observation of serum cytokines in patients receiving hand transplantation]. (2001)

The predominance of Th2 over Th1 confirms the efficiency of immunosuppressive drug regimens, which contribute to the survival of hand transplant. Hand transplantation does not elicit significant immune rejection. The dynamic determination of cytokines helps judge the prognosis and diagnosis of rejection in hand transplantation.
Replica Filter Screening Technique to Detect Transfected Cells Expressing β 2 -Adrenergic Receptor (1989)

We have utilized a replica transfer technique to develop a novel screening assay for the identification of transfectants expressing beta 2-adrenergic receptors (beta 2-AR). The hamster beta 2-AR gene flanked by either its natural promoter or the zinc-inducible mouse metallothionein (MMT) promoter was cotransfected with plasmids conferring neomycin resistance (pRSVneo) into beta 2-AR-deficient mouse L cells. Transfectant colonies were grown on polyester nylon filters and screened by filter binding with [125I]iodohydroxybenzylpindolol to identify colonies expressing beta 2-AR. Individual colonies were isolated and examined to determine beta 2-AR gene dosage, mRNA expression, and receptor densities and affinities. Analysis of cell lines expressing beta 2-AR indicates that this method can identify transfectants containing only a single beta 2-AR gene copy and expressing as few as 4,000 beta 2-receptors per cell. This method may be useful as a tool for the molecular cloning of neurotransmitter receptor genes and for the measurement of transfection efficiencies and expression of receptor genes in cells.
Real-time Multidimensional Temporal Analysis of Complex High Volume Physiological Data Streams in the Neonatal Intensive Care Unit (2013)

The intensive care of immature preterm infants is a challenging, dynamic clinical task that is complicated because these infants frequently develop a range of comorbidities as they grow and develop after their premature birth. Earliest reliable condition onset detection is a goal within this setting and high frequency physiological analysis is showing potential new pathophysiological indicators for earlier onset detection of several conditions. To realise this, a platform for multi-stream, multi-condition, multi-feature risk scoring is required. In this paper we demonstrate our multi-stream online analytics approach for condition onset detection and demonstrate a user interface approach for patient state that can be available in real-time to support condition risk scoring.
Abnormal cavity; abnormal fertility? (2015)

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Implementation issues in a multi-stage feed-forward analog neural network (2003)

Feed-forward multi-layer networks are used in conjunction with a variety of learning algorithms in a wide set of classification problems. Two of the major limitations on the size of hardware implementations are massive interconnectivity and the constraint of designing the whole network on a single substrate. An architecture is discussed that circumvents these problems and provides for simple interchip connections without sacrificing generality. Special attention is given to the practical problems of units and scales in the building blocks and the interfacing of successive modules when the system is decomposed into several sections, each on a separate chip.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Experimental and ab‐initio studies of temperature dependent InN decomposition in various ambient (2008)

Abstract N‐polarity InN layers grown on (0001) sapphire substrates by hydride vapor phase epitaxy (HVPE) were annealed in atmospheric N 2 , H 2 , H 2 + NH 3 , or N 2 + NH 3 ambient at various temperatures. In the N 2 ambient, InN decomposition became significant above 600 °C, whereas in the H 2 ambient, InN layers reacted with H 2 and decomposed at very low temperatures above 350 °C. The process of InN decomposition was also investigated using a first‐principles calculation. In a system free from H 2 , the rate‐limiting process was found to be desorption of N atoms from the surface as atomic N (N*), whereas in the H 2 ambient, the desorption of In atoms as In, InH or InH 3 is the rate‐limiting process. Thus, the decomposition reactions of InN layers in the ambient in the presence and absence of H 2 are different. (© 2008 WILEY‐VCH Verlag GmbH &amp; Co. KGaA, Weinheim)
Trends in diabetes and obesity in Samoa over 35 years, 1978–2013 (2016)

This is the first study to produce trends of Type 2 diabetes mellitus and obesity in Samoa based on standardized data from population surveys. Type 2 diabetes mellitus is equally prevalent in both sexes, and obesity is widespread. Type 2 diabetes mellitus prevalence in Samoa is likely to continue to increase in the near future.
20 GHz phased array antenna for GEO satellite communications (2002)

A phased array antenna is under development by Harris Government Communication Systems Division (GCSD), to provide geostationary Earth orbit (GEO) spacecraft the capability to transfer large volumes of data via a space-to-ground link in the 20.20 to 21.20 GHz band. Phased array antennas provide certain advantages over reflector antennas, and may therefore be considered a desirable option for a GEO spacecraft. Because the beam can be steered electronically, no gimballing is required, permitting agile beam repositioning at extremely high rates. This permits a single antenna to communicate with multiple spatially distributed ground stations by agilely repositioning (hopping) the antenna beam from user to user. A phased array antenna can be positioned on a spacecraft to avoid the need for deployment. The distributed amplifiers constituting the active array provide a failure tolerant architecture. The key components of the antenna are also described.
The minimum energy-delay property of 2-D minimum-phase filters (1982)

A basic property of one-dimensional (1-D) minimum-phase filters is the minimum energy-delay property that of all causal filters having the same magnitude frequency response, the unit-sample response of the minimum-phase filter has its energy concentrated closest to the origin. This note develops a generalization of this property for two-dimensional (2-D) minimum-phase filters.
Catalytic Enantioselective Intramolecular C(sp3)−H Amination of 2‐Azidoacetamides (2018)

An enantioselective ring-closing C(sp<sup>3</sup> )-H amination of 2-azidoacetamides is catalyzed by a chiral-at-metal ruthenium complex and provides chiral imidazolidin-4-ones in 31-95 % yield, with enantioselectivities of up to 95 % ee, and at catalyst loadings down to 0.1 mol % (turnover number (TON)=740). To our knowledge, this is the first example of a highly enantioselective C(sp<sup>3</sup> )-H amination with aliphatic azides. Mechanistic experiments reveal the importance of the amide group, which presumably enables initial bidentate coordination of the 2-azidoacetamides to the catalyst. DFT calculations show that the transition state leading to the major enantiomer features a better steric fit and favorable π-π stacking between the substrate and the catalyst framework.
Capillary electrophoresis with three-color fluorescence detection for the analysis of glycosphingolipid metabolism (2012)

A capillary electrophoresis system with an ultrasensitive three-color laser-induced fluorescence detector was constructed for the simultaneous measurement of glycosphingolipids conjugated with a family of BODIPY fluorophores. The compounds were separated by capillary electrophoresis and detected by laser-induced fluorescence excited within a sheath-flow cuvette. Diode-pumped solid-state lasers operating at 473 nm and 532 nm, and a diode laser operating at 633 nm were used to excite glycosphingolipids tagged with BODIPY–FL, BODIPY–TMR, and BODIPY–650/665 fluorophores. Detection limits were 34 ± 1 molecules, 67 ± 7 molecules, and 36 ± 13 molecules of BODIPY–FL, BODIPY–TMR, and BODIPY–650/665 labeled glycosphingolipids. Separation efficiencies were typically one million theoretical plates. To test the ability of the system to analyze cellular contents in an in vitro biological model, differentiated PC12 cells were co-incubated with BODIPY–FL, BODIPY–TMR, and BODIPY–650/665 labeled lactosylceramide substrates. Cells were homogenized. The metabolic products originating from the glycosphingolipid substrates were simultaneously analyzed using the system.
The Band 3 Receiver Cartridges for ALMA (2006)

Peer reviewed: Yes
Transient Analysis of Acoustically Derived Pressure and Rate Data (1988)

Summary A pressure-buildup test conducted on a sucker-rod pumping well is often distorted by long-duration wellbore storage. In fact, this distortion could be so severe that even a week′s shut-in period may not allow a semilog analysis. A longer shut-in period becomes economically discouraging because of lost production. Low energy and low transmissivity in the reservoir, coupled with increased fluid compressibility, contribute to this long-duration storage phenomenon. One way of reducing the storage effect clearly lies in the simultaneous analysis of downhole pressure and flow rate, estimated from casinghead pressure and rising annular liquid-level measurement made by acoustic well sounding (AWS). Ascertaining the quality of the indirectly measured pressure and rate data constitutes one of the objectives of this study. Several methods exist to translate the AWS measurement to downhole pressure and rate data for the subsequent transient analysis. We show that even an empirical hydrodynamic correlation provides satisfactory transient-pressure/flow-rate data for convolution and deconvolution analyses for moderate pumping-liquid columns. When long annular liquid columns are encountered, translating the AWS measurement with a mechanistically based hydrodynamic model appears to be a prudent approach. Interpretation of several transient tests show that automated convolved-type-curve or history matching of field data is a powerful tool for reservoir-parameter (total mobility, skin, fracture half-length, and storage coefficient) estimation. Use of downhole rate for the convolved type-curve matching reduces the variable storage coefficient by several orders of magnitude, thereby enhancing the quality of the estimates. Deconvolution of downhole pressure and rate data and the pressure-derivative approach significantly aided in well/reservoir flow-model identification. A simple algorithm for computing the Laplace transform of the wellbore pressure for an infinite-conductivity vertically fractured well in an infinite reservoir is developed in this work for a rapid, iterative-type computation used in automated convolved-type-curve analysis.
Design of a Lightpipe Device for Photodynamic Therapy of the Oral Cavity (2010)

The non-imaging methodology developed to design an efficient and compact lightpipe-based device for superficial photodynamic therapy of the oral cavity is reported, together with a study of the fabrication feasibility of the device.