gebhart/patrepeval huggingface dataset:
Aronia melanocarpa Extract Fermented by Lactobacillus plantarum EJ2014 Modulates Immune Response in Mice (2021)

The present study aimed to assess the immunomodulatory effects of fermented <i>Aronia melanocarpa</i> extract (FAME) on RAW 264.7 cells and BALB/c mice. <i>Aronia melanocarpa</i> fruit was fermented with <i>Lactobacillus plantarum</i> EJ2014 by adding yeast extract and monosodium glutamate for 9 days at 30 °C to produce γ-aminobutyric acid (GABA). After fermentation, significant GABA production was noted, along with minerals, polyphenols, and flavonoids (<i>p</i> < 0.05). The polyphenol content was confirmed by liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis. RAW 264.7 cells were stimulated with lipopolysaccharide (LPS, 1 μg/mL) in the presence or absence of FAME, and proinflammatory cytokine contents were measured by qPCR. In the in vivo experiment, female BALB/c mice were administered 125, 250, and 500 mg/kg of FAME for 21 days. FAME treatment increased neutrophil migration and phagocytosis (<i>p</i> < 0.05). It also increased splenocyte proliferation, CD4<sup>+</sup> and CD8<sup>+</sup> T-cell expression, and lymphocyte proliferation. Furthermore, it increased IFN-γ, IL-2, and IL-4 cytokine levels in a dose-dependent manner (<i>p</i> < 0.05). However, it decreased TNF-α and IL-6 levels (<i>p</i> < 0.05). These results indicate that FAME fortified with GABA including bioactive compounds exerts anti-inflammatory effects by inhibiting proinflammatory cytokines in RAW 264.7 cells and modulates immune response in mice. Thus, FAME could be a potential therapeutic agent for inflammatory disorders.
Use of Patient Health Records to Quantify Drug-Related Pro-arrhythmic Risk (2020)

There is an increasing expectation that computational approaches may supplement existing human decision-making. Frontloading of models for cardiac safety prediction is no exception to this trend, and ongoing regulatory initiatives propose use of high-throughput <i>in vitro</i> data combined with computational models for calculating proarrhythmic risk. Evaluation of these models requires robust assessment of the outcomes. Using FDA Adverse Event Reporting System reports and electronic healthcare claims data from the Truven-MarketScan US claims database, we quantify the incidence rate of arrhythmia in patients and how this changes depending on patient characteristics. First, we propose that such datasets are a complementary resource for determining relative drug risk and assessing the performance of cardiac safety models for regulatory use. Second, the results suggest important determinants for appropriate stratification of patients and evaluation of additional drug risk in prescribing and clinical support algorithms and for precision health.
Divergent Management Strategies for Typical Versus Atypical Carcinoid Tumors of the Thoracic Cavity (2013)

These data support the appropriateness of divergent management strategies for typical versus atypical bronchial carcinoids. We propose the following: (1) nonanatomic resection is acceptable only for peripheral typical carcinoids; (2) extended mediastinal dissection should be limited to central presentations, clinically aggressive, or atypical carcinoids; (3) atypical histology, especially with nodal involvement, is prognostic for recurrence and metastasis; (4) nonsurgical therapies only rarely achieve long-term freedom from disease.
Developmental phenomics suggests that H3K4 monomethylation confers multi-level phenotypic robustness (2022)

How histone modifications affect animal development remains difficult to ascertain. Despite the prevalence of histone 3 lysine 4 monomethylation (H3K4me1) on enhancers, hypomethylation appears to have minor effects on phenotype and viability. Here, we genetically reduce H3K4me1 deposition in Drosophila melanogaster and find that hypomethylation reduces transcription factor enrichment in nuclear microenvironments, disrupts gene expression, and reduces phenotypic robustness. Using a developmental phenomics approach, we find changes in morphology, metabolism, behavior, and offspring production. However, many phenotypic changes are only detected when hypomethylated flies develop outside of standard laboratory environments or with specific genetic backgrounds. Therefore, quantitative phenomics measurements can unravel how pleiotropic modulators of gene expression affect developmental robustness under conditions resembling the natural environments of a species.
Inhibition of p38 MAPK by SCIO-469 Suppresses TNF Generation and Promotes CD34+ Cell Survival in an In Vitro MDS Cell Culture Model. (2004)

Abstract Progress in the development of more effective therapeutics for myelodysplastic syndrome (MDS) has been limited by the lack of targets critical to the pathobiology of the disease. Ineffective hematopoiesis in MDS is characterized by accelerated proliferation and premature apoptotic death of progenitors and their progeny that is potentiated by the local generation of inhibitory molecules, including TNFa, TGFß, FasL, and VEGF. To identify upstream regulatory signals that may coordinate activation of inhibitory molecules, we used an in vitro cell culture model incorporating a CD34+ MDS cell line isolated from a RAEB-t patient, normal bone marrow stromal cells (BMSC), and/ or bone marrow mononuclear cells (BMMNC) to determine effects of cell-cell interactions on secretion of inhibitory hematopoietic cytokines. The role of p38 MAP kinase, a regulatory kinase involved in the convergence of inhibitory cytokine activation and signaling, was evaluated in this interaction. We found that p38 MAPK is induced under basal culture conditions in the MDS cell line and is further activated by TNFa or TGFß. In all cases, p38 activation was reduced by SCIO-469, a potent and specific inhibitor of p38a activity. SCIO-469 does not directly block p38 activation, suggesting a feedback loop is interrupted when p38 kinase activity is inhibited in MDS cells. To determine the effects of cellular interactions, the MDS cell line was co-cultured with either BMSC, BMMNCs or both from normal donors, and TNFa and FasL secretion were measured after 3 days incubation. TNFa and FasL were detected in culture supernatants when the MDS cell line was co-cultured with BMMNC but not when co-cultured with BMSC. TNFa secretion by BMMNCs was dependent on MDS cell contact and was significantly inhibited by SCIO-469. The addition of BMSC to the MDS and BMMNC co-culture prevented TNFa elevation, suggesting BMSCs as a dominant source for anti-inflammatory signal(s). VEGF, FGF-ß, TGFß2, BDNF, TIMP-1, TIMP-2 and IL-6 secretion by BMSC was induced by MDS co-culture, whereas SCIO-469 blocked cytokine induction. To determine the effects of SCIO-469 and MDS clone-induced BM cytokine secretion on normal CD34+ proliferation, we co-cultured BMMNCs and BMSC in transwell inserts in the presence or absence of the MDS cell line with or without SCIO-469. CD34+ proliferation was assessed in cells cultured in outer wells. CD34+ progenitors proliferated in culture at the same rate as those co-cultured with BMSC, BMMNC and MDS for 6 days. At longer intervals, viability of progenitors cultured with the MDS line declined, whereas treatment with SCIO-469 abrogated the decrease in CD34+ viability. These results implicate p38a as a critical target in the induction of pro-apoptotic cytokines in MDS, and that selective inhibition of p38 by SCIO-469 may provide a novel therapeutic strategy for MDS.
Electron beam and UV cationic polymerization of glycidyl ethers – PART I: Reaction of monofunctional phenyl glycidyl ether (2013)

Abstract Investigations of electron beam (e‐beam) and ultra violet (UV)‐induced cationic polymerization kinetics of a mono‐functional epoxy system, phenyl glycidyl ether (PGE), were conducted using real time in situ near infrared (NIR) spectroscopy. Effects of processing variables such as temperature and dose rate on initiation and propagation rate constants have been assessed. The experimental results and results from a simple mathematical model developed to predict the reaction behavior under continuous irradiation showed very good agreement. This work provides a basis for investigating the cure behavior of more complex and industrially relevant crosslinking epoxy systems where diffusion limitations play an important role as discussed in PART II. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
C-Reactive Protein, Left Ventricular Mass Index, and Risk of Cardiovascular Disease in Essential Hypertension (2007)

We examined the association between C-reactive protein (CRP) and left ventricular mass index (LVMI), and investigated prospectively the incidence of cardiovascular disease (CVD) in asymptomatic subjects with essential hypertension. A total of 629 subjects (mean age 62 years, 51% female) free of prior CVD were included in this study. In cross-sectional analysis at baseline, patients were divided into three groups according to serum CRP levels: <1, 1 to 2, and >2 mg/L. In multivariate analysis, LVMI increased in a stepwise fashion with increasing CRP levels in both men (127.2+/-2.9, 138.7+/-4.1, 141.8+/-3.5 g/m(2), respectively; F=6.85, p=0.001) and women (119.5+/-3.6, 129.2+/-4.9, 130.2+/-4.8 g/m(2); F=4.23, p=0.031). During follow-up (mean 32 months), 52 subjects (19 female) developed CVD. Kaplan-Meier analysis with log-rank tests showed a significantly poorer event-free survival rate in the group with elevated CRP levels (> or =1 mg/L) (chi(2)=8.22, p<0.01) and that with left ventricular hypertrophy (LVH) (chi(2)=19.91, p<0.01). When participants were divided into four groups on the basis of CRP level (<1 or > or =1 mg/L) and the absence or presence of LVH, the group with LVH/CRP> or =1 mg/L showed markedly poorer event-free survival (chi(2)=28.02, p<0.01), and the adjusted hazard ratio by multivariate Cox regression analysis was 2.65 (95% confidence interval [CI]=1.55-5.46, p<0.01). In the subgroup with LVH (n=362), a significantly lower event-free survival rate of CVD was also observed in the group with CRP> or =1 mg/L (hazard ratio [HR] 1.37, 95% CI: 1.02-1.85, p=0.025). Our findings demonstrate that the CRP level is independently associated with LVMI, and suggest that measurement of CRP may provide clinically important prognostic information to supplement LVH.
MP57-10 ASSOCIATION BETWEEN PREOPERATIVE INITIAL PSA AND NADIR PSA AFTER ROBOT-ASSISTED RADICAL PROSTATECTOMY IN HIGH RISK PROSTATE CANCER PATIENTS (2016)

METHODS: The retrospective study consisted of 1753 men who underwent RP at six independent high volume centers from 2005 to 2011. Of these patients, 306 met the AS criteria that included clinical stage T1c/T2 disease, serum PSA 10 ng/ml, Gleason score 6, and 2 positive cores. The clinic-pathological features were compared between patients with and without 0 reclassification 0 and 0 adverse pathology 0 after RP.
Data from Relaxin-Expressing, Fiber Chimeric Oncolytic Adenovirus Prolongs Survival of Tumor-Bearing Mice (2023)

&lt;div&gt;Abstract&lt;p&gt;Selective replication of oncolytic viruses in tumor cells provides a promising approach for the treatment of human cancers. One of the limitations observed with oncolytic viruses currently used in the treatment of solid tumors is the inefficient spread of virus throughout the tumor mass following intratumoral injection. Data are presented showing that oncolytic adenoviruses expressing the &lt;i&gt;relaxin&lt;/i&gt; gene and containing an Ad5/Ad35 chimeric fiber showed significantly enhanced transduction and increased virus spread throughout the tumor when compared with non–relaxin-expressing, Ad5-based viruses. The increased spread of such viruses throughout tumors correlated well with improved antitumor efficacy and overall survival in two highly metastatic tumor models. Furthermore, nonreplicating viruses expressing relaxin did not increase metastases, suggesting that high level expression of relaxin will not enhance metastatic spread of tumors. In summary, the data show that relaxin may play a role in rearranging matrix components within tumors, which helps recombinant oncolytic adenoviruses to spread effectively throughout the tumor mass and thereby increase the extent of viral replication within the tumor. Expressing relaxin from Ad5/Ad35 fiber chimeric adenoviruses may prove a potent and novel approach to treating patients with cancer. [Cancer Res 2007;67(9):4399–406]&lt;/p&gt;&lt;/div&gt;
Supplementary Tables 1 and 2 and Supplementary Figures 1 through 3 from Systemic Administration and Targeted Radiosensitization via Chemically Synthetic Aptamer–siRNA Chimeras in Human Tumor Xenografts (2023)

&lt;p&gt;Supplementary Tables: Supplementary Table S1. Sequence of Chemically Synthesized Aptamers and RNA Supplementary Table S2. Sequences of primers for qRT-PCR and 5'RACE. Supplementary Figures: Supplementary Figure S1. 5'RACE analysis of Aptamer siRNA chimera directed cleavage of DNA-PK. Supplementary Figure S2. Stability of A10-3-DNAPK in human and mouse serum. Supplementary Figure S3. Systemic DNA-PK silencing by Aptamer-siRNA chimera.&lt;/p&gt;
Codes for differential signaling with many antennas (2002)

We construct signal constellations for differential transmission with multiple basestation antennas. The signals are derived using the theory of fixed-point-free groups and are especially suitable for mobile cellular applications because they do not require the handset to have more than one antenna or to know the time-varying propagation environment. Yet we achieve full transmitter diversity and excellent performance gains over a single-antenna system.
Fluctuation Effects on the Brush Structure of Mixed Brush Nanoparticles in Solution (2018)

A potentially attractive way to control nanoparticle assembly is to graft one or more polymers on the nanoparticle, to control the nanoparticle-nanoparticle interactions. When two immiscible polymers are grafted on the nanoparticle, they can microphase separate to form domains at the nanoparticle surface. Here, we computationally investigate the phase behavior of such binary mixed brush nanoparticles in solution, across a large and experimentally relevant parameter space. Specifically, we calculate the mean-field phase diagram, assuming uniform grafting of the two polymers, as a function of the nanoparticle size relative to the length of the grafted chains, the grafting density, the enthalpic repulsion between the grafted chains, and the solvent quality. We find a variety of phases including a Janus phase and phases with varying numbers of striped domains. Using a nonuniform, random distribution of grafting sites on the nanoparticle instead of the uniform distribution leads to the development of defects in the mixed brush structures. Introducing fluctuations as well leads to increasingly defective structures for the striped phases. However, we find that the simple Janus phase is preserved in all calculations, even with the introduction of nonuniform grafting and fluctuations. We conclude that the formation of the Janus phase is more realistic experimentally than is the formation of defect-free multivalent mixed brush nanoparticles.
TU‐H‐CAMPUS‐TeP3‐02: In‐Situ Dose Painting Using Gold Nanoparticles Released From Cylindrically Shaped Fiducials During External Beam Radiation Therapy (2016)

Purpose: Recent studies have shown that the presence of Gold Nanoparticles (GNPs) in tumor tissue can lead to significant dose enhancement (DE) during External Beam Radiation Therapy (EBRT). In this in‐silico study we investigate EBRT with in‐situ dose painting using GNPs released from cylindrically shaped GNP‐loaded fiducials. Methods: Reported Biologically Target/Tumor Volumes (BTVs) for 12 prostate carcinoma patients were employed in this study. Distribution of the GNPs after burst release from the fiducial (1.5mm diameter and 5mm length) located in the center of the spherically assumed BTV were modeled by isotropic and free diffusion without boundary condition and under the assumption of superposition. An experimentally determined diffusion coefficient for 10nm nanoparticles was adapted for investigating other GNP sizes (2, 5, 15, and 20nm) using the Stokes‐Einstein equation. The maximum size of GNPs to achieve a minimal DE Factor (DEF) of 1.1 for 6MV EBRT using a fiducial‐load of 30mg/g was calculated for typical periods of 14 and 21 days after implantation. Further, the minimal fiducial‐load needed to achieve a clinically significant DEF of 1.2 was computed for 2nm GNPs. Results: Results showed that a minimal DEF of 1.1 could be reached for the smallest patient BTV using a maximal GNP size of 10nm and 20nm after 14 and 21 days, respectively. With increasing BTV smaller GNPs are required to ensure the same DEF. In particular, the largest BTV requires 2nm GNPs for periods of 14 and 21 days. Meanwhile, the required fiducial‐load to reach a minimal DEF of 1.2 after 14 days was found in the range of 17mg/g and 59mg/g for all reported BTVs. Conclusion: This preliminary study indicates a strong dependence on GNP size and fiducial‐load to realize a significant DE. The findings avail further research towards development of GNP‐loaded fiducials for significantly enhancing radiotherapy for cancer patients.
Maximum de vraisemblance non-paramétrique avec régions de censure. Application à un modèle biophysique de décompression (2014)

National audience
Immunoreactive Dog C-Peptide Level in the Pancreatic Vein (1980)

C-peptide immunoreactivity (CPR) levels were measured in dog superior pancreaticoduodenal vein using synthetic dog C-peptide and its antiserum. The basal CPR level was approximately twice as high as the basal immunoreactive insulin (IRI) level on a molar basis. Glucose (10 mg/kg/min) or arginine (250 mg/kg/min) infusion for 5 min into the superior pancreaticoduodenal artery caused a prompt, parallel increase in IRI and CPR. IRI and CPR were closely equimolar at peak secretions. One bolus administration of synthetic neurotensin (10 microgram/kg) into the same artery produced a mild hyperglycemic response and biphasic IRI and CPR responses at 30 min in the vein. The IRI and CPR increases were closely equimolar during the first phase of secretion, but during the second peak a larger increase was found in CPR than IRI. Upon infusion of synthetic substance P (50 ng/kg/min) for 30 min, IRI and CPR concentrations showed a parallel and closely equimolar fall. These results indicate that insulin and C-peptide were released from beta cells in equimolar concentrations.
The Usability of Perforator-based Fasciocutaneous Flap for Trochanteric Pressure Sore (2007)

Purpose: Myocutaneous flap was widely used for trochanteric pressure sore but it had many drawbacks such as donor site morbidity, dog-ear deformity and functional muscle sacrifice. We have performed fasciocutaneous flap based on perforating vessels and succeeded in overcoming its drawbacks.Methods: We experienced 11 cases of perforator- based fasciocutaneous flap for the coverage of trochanteric pressure sore in 9 patients, 2 cases of which were bilateral. The ambulatory status of patient group is as follows: 6 of them used a wheelchair, 2 of them are free walking, 1 of them use a wheelchair or crutches. Flap was supplied by cutaneous perforating vessel of descending branch of the lateral circumflex femoral artery and the third perforating artery of the deep femoral artery. The size of wounds were from 4× 6.5cm to 10×13cm.Results: We did not find any flap loss or congestion except 2 partial wound dehiscences and 1 wound infection. Donor site morbidity was not found. We observed no recurrence of the pressure sore during the 2.5 year follow-up period.Conclusion: We considered that perforator-based fasciocutaneous flap could overcome the traditional drawbacks of the conventional myocutaneous flap and its modified flap for trochanteric pressure sore. And this flap has many advantages for covering trochanteric pressure sore without any donor site deformity and morbidity, which would greatly improve the aesthetic result.
ChemInform Abstract: MULTIPLE METAL‐CARBON BONDS. 34. METATHESIS OF ACETYLENES BY MOLYBDENUM(VI) ALKYLIDYNE COMPLEXES (1984)

Abstract Untersuchungen zeigen, daß die wie auf gezeichnet synthetisierten Neopentylidin‐Mo‐Komplexe (III) mit den sperrigen und elektronenanziehenden fluorierten Alkoxid‐ und Phenoxid‐Liganden schnell mit Dialkylacetylenen wie (IV) unter Bildung neuer Alkylidin‐Mo‐Komplexe z.B. (VI) und neuer tert.‐Butyl‐enthaltender Acetylene, z.B. (V), reagieren.
Engineering agglomeration and propagation of high Al/CuO nanothermite loading composites with reactive and non-reactive fibers (2022)

View Video Presentation: https://doi.org/10.2514/6.2022-1256.vid Nanometallic fuels based energetic nanocomposites (such as nanothermites) are attracting increasing attention for their high enthalpy of reaction. However, the energy density and energy release rate are limited by (1) difficulty of loading high content of nanoparticles; (2) rapid loss of nanostructure during reaction (agglomeration). In this paper, we employ either reactive (CuO wires) or non-reactive fibers (carbon fibers) to reinforce Al/CuO nanocomposite via a simple direct writing approach with a 90 wt% particle loading. Microscopic combustion of the above composites is observed at ~ µs time and ~ µm spatial resolution with pyrometry, revealing a significant reduction in the agglomeration size of the aluminum nanoparticles (Al NPs) and higher propagation rates of the composites with both reactive and non-reactive fibers. Based on these findings, we found that the reduced agglomerations provide enhanced heat feedback to the unreacted materials thus promote the flame propagation. This study outlines how these approaches may enhance the propagation and reduce the two-phase flow losses.
Graded magnetic component and method of forming (2023)

A magnetic component including at least one region is disclosed. The at least one region includes nitrogen and a concentration of the nitrogen in the at least one region is graded across a dimension of the at least one region. Further, a saturation magnetization in the at least one region is graded across the dimension of the at least one region. Further, a method of varying the magnetization values in at least one region of the magnetic component is disclosed.