gebhart/patrepeval huggingface dataset:
Regulation of transcervical permeability by two distinct P2 purinergic receptor mechanisms (2002)

Micromolar concentrations of ATP stimulate biphasic change in transepithelial conductance across CaSki cultures, an acute increase (phase I response) followed by a slower decrease (phase II response). Phase I and phase II responses involve two distinct calcium-dependent pathways, calcium mobilization and calcium influx. To test the hypothesis that phase I and phase II responses are mediated by distinct P2 purinergic receptors, changes in permeability were uncoupled by blocking calcium mobilization with 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA) or by lowering extracellular calcium, respectively. Under these conditions ATP EC(50) was 25 microM for phase I response and 2 microM for phase II response. The respective agonist profiles were ATP > UTP > adenosine 5'-O-(3-thiotriphosphate) (ATP-gamma S) = N(6)-([6-aminohexyl]carbamoylmethyl)adenosine 5'-triphosphate (A8889) > GTP and UTP > ATP > GTP = A8889 > ATP-gamma S. Suramin blocked phase I response and ATP-induced calcium mobilization, whereas pyridoxal phosphate-6-azophenyl-2',4-disulfonic acid (PPADS) blocked phase II response and ATP-augmented calcium influx. ATP time course and pharmacological profiles for phase II response and augmented calcium influx were similar, with a time constant of 2 min and a saturable concentration-dependent effect (EC(50) of 2-3 microM). RT-PCR experiments revealed expression of mRNA for both the P2Y(2) and P2X(4) receptors. These results suggest that the ATP-induced phase I and phase II responses are mediated by distinct P2 purinergic receptor mechanisms.
Bispecific T-Cell Engager (BiTE) Antibody Construct Blinatumomab for the Treatment of Patients With Relapsed/Refractory Non-Hodgkin Lymphoma: Final Results From a Phase I Study (2016)

In this phase I study of relapsed/refractory NHL, continuous infusion with CD19-targeted immunotherapy blinatumomab at various doses and schedules was feasible, with an MTD of 60 μg/m(2)/day. Single-agent blinatumomab showed antilymphoma activity.
A Hermetically Sealed, Fluid-Filled Surgical Enclosure for Microgravity (2013)

Evaluation of the AISS in reduced gravity corroborates observations in the literature regarding the difficulty in maintaining visualization of the surgical field when performing procedures in an air environment. By immersing the surgical field in fluid we were able to apply suction directly to the hemorrhage and also achieve hemostasis.
Small-Form-Factor Optical Phased Array Module for Technology Adoption in Custom Applications (2019)

We present a small-form-factor optical phased array module with a 512-element array, driving CMOS ASICs, and interfacing FPGA. This 80×40×20mm3 module enables unprecedented evaluation and adoption of optical phased array technology for custom applications. © 2019 The Author(s)
Experimental and theoretical study of gramicidin P, an analog of gramicidin A with a methylamine C‐terminal (1987)

Gramicidin P (a gramicidin A in which the ethanolamine C-terminus is replaced by methylamine) was synthesized and shown to have the same single-channel conductance behavior as gramicidin A. The results are discussed in connection with the energy profile computed in the presence of water in comparison with the corresponding profile for gramicidin A.
Signaling Scaffolds in Cardiovascular Development and Disease (2011)

Background: G-protein signaling pathways regulate many aspects of cardiovascular development and disease. A-kinase anchoring proteins (AKAPs) are scaffolding molecules that coordinate and integrate these signaling events into specific physiological processes. One family member, AKAP13, integrates Gs, Gq/11, and G12/13 signals through binding of protein kinase A, C (PKC), and D (PKD) and encoding an active Rho-guanine nucleotide exchange factor (Rho-GEF) domain. AKAP13 is required for mouse development as null embryos die by E10.5 and exhibit cardiovascular defects. Additionally, the Rho-GEF and PKC-PKD binding domains mediate cardiomyocyte hypertrophy in isolated cardiomyocytes. However, the specific developmental processes regulated by AKAP13 and the requirements for the Rho-GEF and PKC-PKD binding domains during development and cardiac hypertrophy are unknown.Methodology/Principal Findings: We used an RNAi, loss-of-function approach, to determine if AKAP13 mediates differentiation of mouse embryonic stem (ES) cells or angiogenic processes in human umbilical vein endothelial cells (HUVECs). We found that AKAP13 knockdown did not decrease ES cell differentiation into Nkx2.5-GFP-positive cardiac mesodermal cells or functionally beating cardiomyocytes. Similarly, the expression of endodermal, endothelial, or cardiac structural marker genes was not affected. We also found that AKAP13-deficient HUVECs had normal tube formation, VEGF-induced wound healing, and VEGF-induced PKD phosphorylation. We then generated AKAP13-truncation mutant mice to determine if the Rho-GEF and PKC-PKD binding domains are required for development. Surprisingly, homozygous mutant mice were born at normal Mendelian ratios and had normal survival, fertility, and cardiac structure. Finally, to determine if these domains mediate β-adrenergic-induced cardiac hypertrophy, we stressed the mice with isoproterenol. We found that heart size increased normally in mice lacking the Rho-GEF and PKC-PKD binding domains. However, these hearts failed to increase cardiac ejection fraction or fractional shortening.Conclusions: These results indicate that AKAP13 is not required for differentiation into cardiovascular tissue, the formation of functional cardiomyocytes, or angiogenic processes. They also indicate that the AKAP13 Rho-GEF and PKC-PKD binding domains are not required for mouse development or the cardiac structural response to β-adrenergic-induced hypertrophy. However, these domains are required for the proper cardiac functional response to β-adrenergic stimulation.
Predicting safety and quality of thermally processed canned foods using a neural network (2004)

An artificial neural network (ANN) for reliably predicting the process temperature (T e ) and process time (t) for minimum quality degradation (F oq ) during thermal processing of canned foods was developed. Five inputs (can size, initial temperature, thermal diffusivity, sensitivity indicator of micro-organism and sensitivity indicator of quality) were used to predict the process variables T e , t, and F oq . A measure of dependency and statistical tests were used to reduce the number of inputs with little degradation of ANN performance. The feedforward ANN showed satisfactory prediction error. The mean relative error (MRE) was 0.2% in predicting T e , 3.9% in predicting t, and 1.5% in predicting F oq . The ANN showed high MRE in predicting the outputs when tested with the radial basis function (RBF) network.
ABL fusion oncogene transformation and inhibitor sensitivity are mediated by the cellular regulator RIN1 (2010)

ABL gene translocations create constitutively active tyrosine kinases that are causative in chronic myeloid leukemia, acute lymphocytic leukemia and other hematopoietic malignancies. Consistent retention of ABL SH3/SH2 autoinhibitory domains, however, suggests that these leukemogenic tyrosine kinase fusion proteins remain subject to regulation. We resolve this paradox, demonstrating that BCR-ABL1 kinase activity is regulated by RIN1, an ABL SH3/SH2 binding protein. BCR-ABL1 activity was increased by RIN1 overexpression and decreased by RIN1 silencing. Moreover, Rin1(-/-) bone marrow cells were not transformed by BCR-ABL1, ETV6-ABL1 or BCR-ABL1(T315I), a patient-derived drug-resistant mutant, as judged by growth factor independence. Rescue by ectopic RIN1 verified a cell autonomous mechanism of collaboration with BCR-ABL1 during transformation. Sensitivity to the ABL kinase inhibitor imatinib was increased by RIN1 silencing, consistent with RIN1 stabilization of an activated BCR-ABL1 conformation having reduced drug affinity. The dependence on activation by RIN1 to unleash full catalytic and cell transformation potential reveals a previously unknown vulnerability that could be exploited for treatment of leukemic cases driven by ABL translocations. The findings suggest that RIN1 targeting could be efficacious for imatinib-resistant disease and might complement ABL kinase inhibitors in first-line therapy.
Distributed Data Fusion for Multirobot Search (2014)

This paper presents novel data fusion methods that enable teams of vehicles to perform target search tasks without guaranteed communication. Techniques are introduced for merging estimates of a target's position from vehicles that regain contact after long periods of time, and a fully distributed team-planning algorithm is proposed, which utilizes limited shared information as it becomes available. The proposed data fusion techniques are shown to avoid overcounting information, which ensures that combining data from different vehicles will not decrease the performance of the search. Motivated by the underwater search domain, a realistic underwater acoustic communication channel is used to determine the probability of successful data transfer between two locations. The channel model is integrated into a simulation of multiple autonomous vehicles in both open water and harbor environments. The results demonstrate that the proposed distributed coordination techniques provide performance competitive with full communication.
L-band multi-wavelength erbium-doped fiber laser (2002)

A wideband multi-wavelength erbium-doped fiber laser in L-band with frequency shifted feedback and gain-loss balancing is presented. Laser channels with 400 GHz spacing over 20 nm bandwidth were demonstrated within 0.8 dB output power excursion.
An optical method to study the domain wall motion in transparent, Perovskite-Type ceramics (1980)

Transparent PLZT 6/65/35 ceramics have been studied by special optical means to obtain detailed results concerning the 71°/109° domain wall oscillation caused by small a.c. electrical fields (E << Ec). Especially, the investigation of modulation of light depolarization has proved to be well suited to determine the amplitude of the domain wall oscillation. It was found that in an a.c. electrical field of 20V/mm the domain walls oscillate with an amplitude of 2-4·10-7 mm around their equilibrium position.
Pre-COVID-19 Immunity to Common Cold Human Coronaviruses Induces a Recall-Type IgG Response to SARS-CoV-2 Antigens Without Cross-Neutralisation (2022)

The capacity of pre-existing immunity to human common coronaviruses (HCoV) to cross-protect against <i>de novo</i> COVID-19is yet unknown. In this work, we studied the sera of 175 COVID-19 patients, 76 healthy donors and 3 intravenous immunoglobulins (IVIG) batches. We found that most COVID-19 patients developed anti-SARS-CoV-2 IgG antibodies before IgM. Moreover, the capacity of their IgGs to react to beta-HCoV, was present in the early sera of most patients before the appearance of anti-SARS-CoV-2 IgG. This implied that a recall-type antibody response was generated. In comparison, the patients that mounted an anti-SARS-COV2 IgM response, prior to IgG responses had lower titres of anti-beta-HCoV IgG antibodies. This indicated that pre-existing immunity to beta-HCoV was conducive to the generation of memory type responses to SARS-COV-2. Finally, we also found that pre-COVID-19-era sera and IVIG cross-reacted with SARS-CoV-2 antigens without neutralising SARS-CoV-2 infectivity <i>in vitro</i>. Put together, these results indicate that whilst pre-existing immunity to HCoV is responsible for recall-type IgG responses to SARS-CoV-2, it does not lead to cross-protection against COVID-19.
Dysgonomonas alginatilytica sp. nov., an alginate-degrading bacterium isolated from a microbial consortium (2015)

Gram-stain-negative, facultatively anaerobic, non-motile, non-spore-forming, rod-shaped bacterium, designated strain HUA-2T, was isolated from an alginate-degrading microbial consortium. Strain HUA-2T was related to Dysgonomonas capnocytophagoides JCM 16697T, Dysgonomonas macrotermitis JCM 19375T and Dysgonomonas mossii CCUG 43457T with 95.1 %, 94.1 % and 92.1 % 16S rRNA gene sequence similarity, respectively. The optimal growth temperature and pH for strain HUA-2T were 35 °C and pH 8.0, respectively. Enzyme production, major fermentation products from glucose, and the major cellular fatty acids were different from those of D. capnocytophagoides CCUG 17966T or other members of the genus Dysgonomonas. Therefore, strain HUA-2T is proposed to represent a novel species of the genus Dysgonomonas, for which we propose the name Dysgonomonas alginatilytica sp. nov. The type strain is HUA-2T ( = DSM 100214T = HUT 8134T).
Non-invasive imaging of islet infiltration reveals an early window of CRIg-dependent diabetes determinism in NOD mice (171.29) (2012)

Abstract All juvenile NOD mice exhibit insulitis, but there is substantial variation in progression to diabetes. We demonstrate that a patient-validated magnetic-resonance-imaging strategy to non-invasively visualize local effects of pancreatic-islet inflammation can predict diabetes onset in NOD mice. MRI signals acquired during a narrow early time-window allowed pre-sorting into disease-progressors and -nonprogressors and an estimate of time-to-diabetes. We exploited this capability to identify novel elements correlated with disease protection. Highlighted was CRIg (complement receptor of the immunoglobulin superfamily), which tagged a subset of macrophages associated with diabetes resistance. Administration of CRIg-Fc to young NODs depressed MRI signals and diabetes incidence. Besides nominating new regulators of disease progression, this study demonstrated that diabetes is set at an early age in NOD mice.
Substrate specificity and carbohydrate synthesis using transketolase (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSubstrate specificity and carbohydrate synthesis using transketolaseYoshihiro Kobori, David C. Myles, and George M. WhitesidesCite this: J. Org. Chem. 1992, 57, 22, 5899–5907Publication Date (Print):October 1, 1992Publication History Published online1 May 2002Published inissue 1 October 1992https://pubs.acs.org/doi/10.1021/jo00048a023https://doi.org/10.1021/jo00048a023research-articleACS PublicationsRequest reuse permissionsArticle Views1361Altmetric-Citations147LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Enhanced Thermal Performance of High Flux LED Systems with Two-Phase Immersion Cooling (2020)

Considering significant worldwide use of electricity for general lighting, the implementation of energy efficient technologies is highlighted in many platforms with the use of light emitting diodes (LEDs). Because of its environmentally friendly nature, LEDs offer a promising solution to minimize inefficient use of energy as the demanding operating conditions pose new challenges. Reduction of lumen output, shorter lifetime and degradation of light characteristics with increasing package temperatures are critical issues that need to be addressed with innovative solutions. Especially in high power LEDs exposed to raised heat fluxes, standard cooling methods fail to remove the dissipated heat effectively. Recently, immersion cooling of LEDs with suspended phosphor particles in a dielectric liquid has been offered as a viable option to dissipate a vast amount of heat, ensuring a uniform distribution of temperature, and removal of local hot spots in a high brightness LED package. Therefore, understanding the fluid flow and particle motion due to natural convection in the package is crucial to improve thermal and optical design of an LED system. Temperature distribution and light extraction of a package can be considerably affected by material characteristics, flow regime and flow direction. In this study, the impact of different heat generation rates of an LED package is investigated considering natural convection currents and corresponding phosphor particle trajectories inside a fluid domain. A discrete phase model of a high-power white LED package is created in order to keep track of individual particles interacting with the carrier fluid and heat flow in a closed LED system. Current findings provide good basis for smart control of phosphor particles to maximize thermal and optical performance of both RGB and white LED packages.
Top: Example of simulation for 100 data points and 5 segments in the regular case (2011)

<b>Copyright information:</b>Taken from "A statistical approach for array CGH data analysis"BMC Bioinformatics 2005;6():27-27.Published online 11 Feb 2005PMCID:PMC549559.Copyright © 2005 Picard et al; licensee BioMed Central Ltd. The true break-points are designated by vertical lines, and the red lines correspond to the mean of each segment. The difference of means is constant and equals 1. Bottom: Estimated frequency for a break-point to be located at coordinate for = 1 to 100. Different levels of noise are considered with = 0.1, = 0.5, = 1.
The iron-based phosphate binder PA21 has potent phosphate binding capacity and minimal iron release across a physiological pH range in vitro (2014)

These studies demonstrate that PA21 has potent phosphate binding capacity and low iron release over a physiologically relevant pH range in the GI tract. These features indicate PA21 could be an effective alternative phosphate binder for CKD patients.
Table S4 from Lentiviral-Driven Discovery of Cancer Drug Resistance Mutations (2023)

&lt;p&gt;Results of LentiMutate on EGFRdel746-750 cDNA packaged using M-RT and osimertinib in the HCC827 cell line. Values shown are the averages of two biological replicates. The fold enrichment of "All nucleotides" is the sum of substitution rates for all nucleotides in the with doxycycline replicates divided by the sum of substitution rates for all nucleotides without doxycycline (parental) at one nucleotide position. Nucleotide w/ max error refers to the highest substitution rate for a single nucleotide at one nucleotide position.&lt;/p&gt;
Abstract 732: Using functional and chemical genomics to identify mechanisms of Enzalutamide resistance in prostate cancer (2015)

Abstract Introduction: Enzalutamide is a recently approved potent androgen receptor antagonist for prostate cancer both in the pre- and post-docetaxel settings. Its action relies upon 3 defined mechanisms of action: 1) blocking testosterone binding to the androgen receptor (AR), 2) preventing nuclear translocation of AR, and 3) inhibiting AR-DNA binding. However, despite its utility in prolonged overall survival in both these settings, disease relapse is inevitable, suggesting that resistance to the drug is somehow being acquired and is of vital concern. Methods: Using a combination of high-throughput functional genomic (kinome-shRNA) and chemical genomic (pharmacological inhibitor) screens, along with various in vitro / in vivo assays (proliferation, apoptosis, necrosis, soft agar growth, etc.) we have identified the IkB kinase (IKK) / IkB / NFkB signaling axis as important for acquisition of, and continued resistance to, Enzalutamide. Results: Using a panel of Enzalutamide-resistant and -sensitive cell lines, we show that targeting this signaling axis will selectively kill Enzalutamide-resistant cells while not affecting the proliferation of sensitive cells. Moreover, our data shows that the pharmacological inhibitors and shRNAs are effective even without Enzalutamide administration, negating any potential drug-drug interaction issues. Finally, we have found that our cells are resistant to Enzalutamide in an AR-V7-independent manner, suggesting that NFkB signaling is acting in a novel manner to bring about the resistance phenotype. Conclusions: Through in vitro and in vivo experiments, we provide evidence that targeting activators of NFκB signalling can be a strategy to help patients with Enzalutamide-resistant prostate cancer. Note: This abstract was not presented at the meeting. Citation Format: Sujeeve Jeganathan, Amina Zoubeidi, Martin Gleave, Brad G. Wouters, Anthony M. Joshua. Using functional and chemical genomics to identify mechanisms of Enzalutamide resistance in prostate cancer. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 732. doi:10.1158/1538-7445.AM2015-732
Silica Functionalized by Bifunctional Dendrimers: Hybrid Nanomaterials for Trapping CO2 (2016)

Three types of poly(phosphorhydrazone) dendrimeric structures bearing function(s) suitable for grafting to silica and trapping CO 2 have been grafted to different types of silica with various textural properties (e.g., different surface areas, porous or nonporous, pore sizes). These hybrid hard/soft and inorganic/organic nanomaterials have been characterized. Some representative examples of these hybrid materials were chosen for trapping CO 2 and their performances have been analyzed.
Section Reviews; Anti-infectives: Section Review Anti-infectives: HIV protease inhibitors in early development (1996)

Over the last ten years, two important intervention points in the life cycle of the human immunodeficiency virus (HIV) which involve two viral-specific enzymes, HIV reverse transcriptase (RT) and HIV protease, have been the target of intense research efforts to identify useful therapeutic agents. Several nucleoside analogues which are RT inhibitors have been approved for use in humans. Several nonnucleoside RT inhibitors are now under development. Within the last twelve months, three different HIV protease inhibitors - saqui-navir, ritonavir and indinavir - have been approved for marketing, thus validating the concept of HIV protease as an important therapeutic target. In this review, several new HIV protease inhibitors that are in early clinical development will be discussed. These compounds are VX-478, AG-1343 (nelfinavir mesylate), palinavir, KNI-272, DMP-450, U-103017 and CGP 61755.
Verification of Digital RF Processors: RF, Analog, Baseband, and Software (2007)

Single-chip RF SoCs are seeing widespread acceptance in wireless applications. In this paper we address the issue of design verification of single-chip RF SOCs in a framework that accepts RF input and analyzes receiver BER performance and transmitter output distortion and phase noise by processing several thousand packets of baseband information while compensation algorithms are simultaneously executed. No comprehensive methodology exists to date for designing such complex systems. This paper present a novel approach that allows building complex RF SoC systems based on VHDL modeling and simulation and opens up major avenues of model development for RF and analog circuits. This approach has been successfully applied to verify two generations of digital RF processors (DRP) in deep-submicron technologies
Light-matter interaction phenomena using subwavelength engineering of material properties. (2015)

Abstract not provided.