gebhart/patrepeval huggingface dataset:
Human neutrophil adherence to thrombospondin occurs through a CD11/CD18-independent mechanism (1991)

Thrombospondin (TSP), a 450-kDa trimeric glycoprotein secreted by platelets and endothelial cells at sites of tissue injury or inflammation, may play an important role in polymorphonuclear leukocyte (PMN) adherence to blood vessel walls before diapedesis. We have examined the adherence of PMN to TSP and compared it to adherence to other extracellular matrix proteins. PMN adherence to TSP-coated plastic was complete by 60 min with spreading completed by 2 h. The kinetics of adhesion and spreading on TSP were similar to that of vitronectin (VN), laminin (LN), and fibronectin (FN). Activation of PMN with the calcium ionophore A23187 or the chemotactic peptide FMLP increased PMN adherence to LN and FN, but not to TSP or VN, suggesting that PMN activation may differentially regulate expression of TSP and VN receptors as compared to LN and FN receptors. The specificity of PMN adherence to TSP was confirmed by competition with saturating amounts of TSP and inhibition with anti-TSP antibodies. mAb A6.1, which binds to the protease-resistant core of TSP, was the most effective in blocking PMN adherence to TSP. Using TSP proteolytic fragments, we demonstrated that the primary interaction of PMN with TSP was mediated through the 140-kDa COOH-terminal domain. Inasmuch as the 140-kDa fragment of TSP contains an Arg-Gly-Asp sequence similar to the cell recognition site of FN and VN, we determined whether RGDS peptides would inhibit PMN adhesion. RGDS did not significantly inhibit PMN adhesion to TSP, VN, or LN, but reduced PMN adhesion to FN by 50%. To determine if PMN adhesion to TSP was mediated by a beta 2 integrin receptor such as LFA-1, MO-1, or p150,95, we performed adhesion assays using PMN isolated from patients with leukocyte adhesion deficiency that lack beta 2 receptors. Leukocyte adhesion deficiency PMN exhibited normal adherence to TSP. In contrast, adherence to VN, LN, and FN was reduced by 95%. Therefore, adherence to TSP is probably not mediated by a beta 2 integrin receptor. These data contribute to the accumulating evidence that PMN can interact with extracellular matrix proteins through a CD11/CD18-independent process.
On the Role of the Shaft End and the Influence of Frame Size and Load Coupling on the RF Emission Characteristics of Induction Motors (2013)

A novel nonintrusive radio-frequency-based method to detect discharge bearing currents occurring in variable-speed drives has been presented previously. Further understanding of the emission characteristics of the discharge and of the electric machine in which it occurs will extend its areas of application. To this aim, this paper addresses the modeling of the machine as an antenna, identifying the shaft ends to play a key role in RF pulse transmissions. Experimental and theoretical studies are carried out for squirrel-cage induction machines of different shaft heights, with the lengths of shafts and loads connected through different forms of coupling.
Three dimensional composites of graphene as supports in Pd-catalyzed synthetic applications (2018)

A facile solid-state method to synthesize a highly active and recyclable Pd catalyst of 3D supports made of Ni, G and, CNTs for Suzuki reaction and C–H activation is presented.
Supplementary Tables 1 - 7 and Figures 1 - 3 from Genetic Evidence of a Precisely Tuned Dysregulation in the Hypoxia Signaling Pathway during Oncogenesis (2023)

&lt;p&gt;Table S1: Phenotypes associated with single R161Q and R200W VHL germline mutations are different from the phenotype associated with the combined mutations. Table S2: VHL mutations associated with polycythemia are different from the VHL mutations involved in severe von Hippel-Lindau disease. Table S3: Members of the family carrying the VHL-R200W+R161Q mutations present normal hematological values. Table S4: Intra-domain hydrogen bonds are severely destabilized by the mutations. Table S5: The double mutant reduced the inter-domain mobility compared to the single one. Table S6: The pVHL/HIF-2α direct target genes are deregulated with a significantly ascending gradient by the pVHL mutants of increased severity. Table S7: The target genes of the pVHL/HIF-2α axis that are deregulated in renal cell carcinoma play a role in oncogenic pathways. Figure S1: Germline mutations in the VHL gene are associated with distinct phenotypes. Figure S2: (A) The crystallographic structure of pVHL contains a seven-stranded β-sandwich connected to four α-helices by a flexible linker. (B) The R200W mutation dramatically weakens hydrogen bonds established in the wild-type form between Arg200 and Glu134, and between Arg197 and Arg120. (C) The R161Q mutation indirectly influences crucial stabilizing interactions involving primarily Arg 167. Figure S3: VHL mRNA expression is equivalent in 786.O cells transduced by the different VHL inducible lentiviral constructs.&lt;/p&gt;
Area-efficient graph layouts (1980)

Minimizing the area of a circuit is an important problem in the domain of Very Large Scale Integration. We use a theoretical VLSI model to reduce this problem to one of laying out a graph, where the transistors and wires of the circuit are identified with the vertices and edges of the graph. We give an algorithm that produces VLSI layouts for classes of graphs that have good separator theorems. We show in particular that any planar graph of n vertices has an O(n lg2 n) area layout and that any tree of n vertices can be laid out in linear area. The algorithm maintains a sparse representation for layouts that is based on the well-known UNION-FIND data structure, and as a result, the running time devoted to bookkeeping is nearly linear.
Seizures (2011)

Anaesthesia for the posterior fossa provides a unique challenge for anaesthetists and neurosurgeons. Optimal patient positioning should facilitate surgical access without compromising patient safety. The important considerations are surgical access, securing and maintaining the airway, maintenance of adequate anaesthetic depth, haemodynamic stability and oxygenation. Care should be taken to limit the 'blackout state' during which the patient is not monitored or connected to the breathing circuits during patient transfer or positioning on the operating table. The hazards during positioning can be reduced by meticulous planning, careful positioning and vigilance to facilitate early detection of complications. The aim of maintenance of anaesthesia is to reduce the intracranial pressure (ICP) and to maintain haemodynamic stability. Anaesthesia can be maintained with either volatile agents or intravenous agents such as propofol. The choice of the anaesthetic agent is at the discretion of the individual anaesthetist.
A prospective study of cardiac valvular status in patients treated with cabergoline for endocrine disease (2012)

peer reviewed
Expression and characterization of Rv0447c product, potentially the methyltransferase involved in tuberculostearic acid biosynthesis in Mycobacterium tuberculosis (2013)

In this study, a previously uncharacterized gene (Rv0447c) of Mycobacterium tuberculosis, designated as an unknown fatty-acid methyltransferase (ufaA1), was cloned, expressed in Escherichia coli, and purified. The biochemical characterization of the purified protein (UfaA1) showed it to be a methyltransferase that catalyzes biosynthesis of the tuberculostearic acid (10-methylstearic-acid, TSA), a significant constituent lipid of the mycobacterial cell wall and a clinical marker of the disease. Here, we show that UfaA1 transfers the methyl group from S-adenosyl-l-methionine (SAM) to the double bond of oleic acid in phosphatidylethanolamine or phosphatidylcholine to produce TSA. Optimal activity was obtained between pH 7.0 and pH 8.0. The methyltransferase activity of UfaA1 was severely inhibited by S-adenosyl-l-homocysteine. The Km values for dioleyl phosphatidylethanolamine, SAM, and nicotinamide adenine dinucleotide phosphate were 14, 13, and 83 µM, respectively, with Vmax of 1.3-1.6 nmol/Min. These results identify the Rv0447c gene product of M. tuberculosis as the methyltransferase that catalyzes the biosynthesis of TSA. This provides new information in mycobacterial cell wall synthesis.
Beyond Antimicrobials (2006)

Pressure ulcers are a major source of morbidity and mortality for older adults, especially frail elders who may be malnourished and have significant comorbid conditions. Successful treatment of pressure ulcers requires coordinated efforts in wound care, pressure relief, and optimization of medical conditions that impact healing, such as diabetes mellitus and congestive heart failure. The role of newer therapies such as growth factors, artificial skin equivalents, and vacuum-assisted closure is growing. Infectious disease physicians should develop a working knowledge of these treatment modalities and the potential advantages and disadvantages of each.
A rapid method for quantifying cytoplasmic versus nuclear localization in endogenous peripheral blood leukocytes by conventional flow cytometry (2017)

A biochemical system and method have been developed to enable the quantitative measurement of cytoplasmic versus nuclear localization within cells in whole blood. Compared with the analyses of nuclear localization by western blot or fluorescence microscopy, this system saves a lot of time and resources by eliminating the necessity of purification and culturing steps, and generates data that are free from the errors and artifacts associated with using tumor cell lines or calculating nuclear signals from 2D images. This user-friendly system enables the analysis of cell signaling within peripheral blood cells in their endogenous environment, including measuring the kinetics of nuclear translocation for transcription factors without requiring protein modifications. We first demonstrated the efficiency and specificity of this system for targeting nuclear epitopes, and verified the results by fluorescence microscopy. Next, the power of the technique to analyze LPS-induced signaling in peripheral blood monocytes was demonstrated. Finally, both FoxP3 localization and IL-2-induced STAT5 signaling in regulatory T cells were analyzed. We conclude that this system can be a useful tool for enabling multidimensional molecular-biological analyses of cell signaling within endogenous peripheral blood cells by conventional flow cytometry. © 2017 International Society for Advancement of Cytometry.
Zeolite-Catalyzed Disproportionation of iso-Propylbenzene: Identification of Reaction Intermediates and Mechanism (2016)

The catalytic properties of a series of large-pore (H–Y, H-beta, H-mordenite, and H-UZM-35) and medium-pore (H-NU-87, H-TNU-9, and H-ZSM-5) zeolites are compared in iso-propylbenzene (iPB) disproportionation. Among the zeolite catalysts studied here, H-UZM-35 with a three-dimensional framework consisting of one type of straight 12-ring channels and two types of tortuous 10-ring channels was found to show a di-iso-propylbenzenes (DiPBs) yield comparable to that of H-beta with two intersecting 12-ring channels, the best catalyst tested for this reaction to date. Gas chromatography–mass spectrometry analysis of used zeolite catalysts demonstrates that while mono-iso-propylated 2,2-diphenylpropane derivatives serve as real reaction intermediates of iPB disproportionation over large-pore zeolites, mono-iso-propenylated 2,2-diphenylpropane species, which contain a double bond in the alkyl chain, are intermediates of its side reaction. Unlike that of other aromatic hydrocarbons such as m-xylene, ethylbenzene, and n-propylbenzene, the formation of di-iso-propylated derivatives was not observed as reaction intermediates. A new bimolecular diphenylpropane-mediated reaction pathway, which includes both intermediates of main and side reactions of iPB disproportionation, is proposed based on the experimental and theoretical results.
Few-mode Multi-core Fibres: Weakly-coupling and Randomly-coupling (2020)

Few-mode multi-core fibre (FM-MCF) design suitable for SDM transmission is described. For weakly-coupled FM-MCF, we explain how to determine fibre parameters and investigate the maximum spatial density in a limited cladding diameter. For randomly-coupled FM-MCF, we discuss how to enhance random mode-mixing between different mode-groups.
Mobile and wireless Internet services: putting the pieces together (2001)

There are a variety of emerging technologies and protocol enhancements designed to extend Internet services to mobile users, including operation over more dynamic, heterogeneous wireless interconnections. Many different approaches and protocols have been proposed, and there are even multiple standardization efforts within the Internet Engineering Task Force that address portions of the overall goal. This article highlights some of the emerging technology efforts and provides insight into how some of these pieces may fit together to realize seamless Internet services for users on the move or in application spaces with little to no preexisting communications infrastructure.
Rate-Splitting Multiple Access for Downlink Multi-Antenna\n Communications: Physical Layer Design and Link-level Simulations (2020)

Rate-Splitting Multiple Access (RSMA) is an emerging flexible, robust and\npowerful multiple access scheme for downlink multi-antenna wireless networks.\nRSMA relies on multi-antenna Rate-Splitting (RS) strategies at the transmitter\nand Successive Interference Cancellation (SIC) at the receivers, and has the\nunique ability to partially decode interference and partially treat\ninterference as noise so as to softly bridge the two extremes of fully decoding\ninterference (as in Non-Orthogonal Multiple Access, NOMA) and treating\ninterference as noise (as in Space Division Multiple Access, SDMA or Multi-User\nMultiple-Input Multiple-Output, MU-MIMO). RSMA has been shown to provide\nsignificant room for spectral efficiency, energy efficiency, Quality-of-Service\nenhancements, robustness to Channel State Information (CSI) imperfections, as\nwell as feedback overhead and complexity reduction, in a wide range of network\nloads (underloaded and overloaded regimes) and user deployments (with a\ndiversity of channel directions, channel strengths and qualities). RSMA is also\ndeeply rooted and motivated by recent advances in understanding the fundamental\nlimits of multi-antenna networks with imperfect CSI at the Transmitter (CSIT).\nIn this work, we leverage recent results on the optimization of RSMA and design\nfor the first time its physical layer, accounting for modulation, coding (using\npolar codes), message split, adaptive modulation and coding, and SIC receiver.\nLink-level evaluations confirm the significant throughput benefits of RSMA over\nvarious baselines as SDMA and NOMA.\n
Enhanced Circular Dichroism and Chiral Sensing with Bound States in the Continuum (2019)

We reveal that optical chirality at the nanoscale can be boosted dramatically by bound states in the continuum (BIC). We predict the enhancement of chiroptical signals from nanostructures supporting quasi-BICs in mid-IR with a record-high efficiency.
Controlled release of 4‐hydroperoxycyclophosphamide from the fatty acid dimer‐sebacic acid copolymer (1992)

Abstract Controlled polymeric release of chemotherapeutic agents has shown promise in the management of malignant gliomas. 4‐Hydroperoxycyclophosphamide (4HC), loaded on the fatty acid dimer–sebacic acid copolymer (FAD:SA, 1:1), significantly prolonged survival in rats implanted with F98 and 9L gliomas. Here, we studied the in vitro and in vivo release kinetics in phosphate‐buffered saline and rat brain of 20% 4HC/FAD:SA (wt:wt), the optimal dose for treatment of rat gliomas. In vitro release under infinite sink conditions was steady over the initial 12 hr to a peak of 20–35% of impregnated drug, consistent with early phase control via surface erosion. Release over the next 3 weeks was minimal, consistent with barrier formation around the polymer by an oily fatty acid dimer degradation product and consequent slowing of release. However, the polymer started to disintegrate by day 4, and there were minimal visible remnants by 3 weeks. Thus, a considerable amount of polymer‐carried drug was probably lost in the disintegrating fragments. Also, drug loss is expected from its inherent hydrolytic instability. In vivo release into brain revealed two peak levels of drug at 0–1 hr and 5–20 days. With loaded polymer implanted intraperitoneally or cyclophosphamide injected systemically, peak brain drug levels were measured in 2–8 hr, with substantial decrease by 48 hr without a second peak. Brain levels were substantially higher than blood levels at all time periods. We conclude that FAD:SA (1:1) adequately protects the otherwise labile 4HC, allowing effective and substained drug release in vivo . Furthermore, it should be possible to modify the polymer to adjust the time of peak release for more beneficial therapeutic effects.
An assessment of the iPad as a testing platform for distance visual acuity in adults (2013)

Tablet computers are an attractive option for visual acuity measurement due to portability, the ability to randomise letters, automated scoring of acuity and the ability to select from a range of charts. However, these devices are only suitable for use in situations where sources of glare can be eliminated.
Late urinary toxicity modeling after stereotactic body radiotherapy (SBRT) in the definitive treatment of localized prostate cancer (2015)

NTCP modeling of late urinary flare after ultra-hypofractionated prostate SBRT demonstrates a relatively small volume effect for dose to the bladder, suggesting that reduction of volume receiving elevated dose will result in decreased incidence of late urinary toxicity. Future studies will be needed to examine the impact of dose to other potential sources of late genitourinary toxicity.
Meta-analysis: Retinal Vessel Caliber and Risk for Coronary Heart Disease (2009)

Retinal vessel caliber changes were independently associated with an increased risk for CHD events in women.
Effect of Carbon Black and Silica Fillers in Elastomer Blends (2001)

The effects of carbon black and pyrogeneous silica fillers on the interfacial properties of a homopolymer [polybutadiene, (PB)] and a terpolymer [brominated poly(isobutylene-co-p-methylstyrene), (BIMS)] are reported. Neutron reflectivity (NR) was used to study the interfacial structure. The results are complemented by scanning transmission X-ray microscopy (STXM) and lateral force microscopy (LFM), which were used to probe the morphology and surface lateral force. Small-angle neutron scattering (SANS) was used to characterize the size and surface properties of the filler aggregates in elastomers. Our results show that the interfacial behavior of PB/BIMS is more sensitive to carbon black than to silica. The interfacial broadening is significantly slowed down with addition of merely φCB = 0.01 (volume fraction) carbon black fillers. This volume level is much lower than that used in bulk rubbers (φCB ≥ 0.1). Pyrogeneous silica has a less pronounced effect on the interfacial characteristics. When both carbon and silica are incorporated into the PB layer, the effect of carbon black is offset by silica fillers.
Separation of diastereomers using a low cost preparative medium-pressure liquid chromatograph (1979)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSeparation of diastereomers using a low cost preparative medium-pressure liquid chromatographA. I. Meyers, Joel Slade, R. Keith Smith, and E. D. MihelichCite this: J. Org. Chem. 1979, 44, 13, 2247–2249Publication Date (Print):June 1, 1979Publication History Published online1 May 2002Published inissue 1 June 1979https://pubs.acs.org/doi/10.1021/jo01327a045https://doi.org/10.1021/jo01327a045research-articleACS PublicationsRequest reuse permissionsArticle Views419Altmetric-Citations48LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Conditional Deletion of Sost in MSC‐derived lineages Identifies Specific Cell Type Contributions to Bone Mass and B Cell Development (2018)

Sclerostin (Sost) is a negative regulator of bone formation and blocking its function via antibodies has shown great therapeutic promise by increasing both bone mass in humans and animal models. Sclerostin deletion in Sost KO mice (Sost-/- ) causes high bone mass (HBM) similar to sclerosteosis patients. Sost-/- mice have been shown to display an up to 300% increase in bone volume/total volume (BV/TV), relative to age-matched controls. It has been postulated that the main source of skeletal sclerostin is the osteocyte. To understand the cell-type specific contributions to the HBM phenotype described in Sost-/- mice, as well as to address the endocrine and paracrine mode of action of sclerostin, we examined the skeletal phenotypes of conditional Sost loss-of-function (SostiCOIN/iCOIN ) mice with specific deletions in (1) the limb mesenchyme (Prx1-Cre; targets osteoprogenitors and their progeny); (2) midstage osteoblasts and their progenitors (Col1-Cre); (3) mature osteocytes (Dmp1-Cre); and (4) hypertrophic chondrocytes and their progenitors (ColX-Cre). All conditional alleles resulted in significant increases in bone mass in trabecular bone in both the femur and lumbar vertebrae, but only Prx1-Cre deletion fully recapitulated the amplitude of the HBM phenotype in the appendicular skeleton and the B-cell defect described in the global KO. Despite WT expression of Sost in the axial skeleton of Prx1-Cre deleted mice, these mice also had a significant increase in bone mass in the vertebrae, but the sclerostin released in circulation by the axial skeleton did not affect bone parameters in the appendicular skeleton. Also, both Col1 and Dmp1 deletion resulted in a similar 80% significant increase in trabecular bone mass, but only Col1 and Prx1 deletion resulted in a significant increase in cortical thickness. We conclude that several cell types within the Prx1-osteoprogenitor-derived lineages contribute significant amounts of sclerostin protein to the paracrine pool of Sost in bone. © 2018 The Authors. Journal of Bone and Mineral Research Published by Wiley Periodicals, Inc.