gebhart/patrepeval huggingface dataset:
Prostaglandin E2 and cAMP inhibit B lymphocyte activation and simultaneously promote IgE and IgG1 synthesis (1992)

Macrophage-secreted prostaglandins of the E series inhibit numerous immunologic events, including IgM secretion by B lymphocytes. In this study, we investigated whether PGE also regulates the activation of normal quiescent murine B cells and subsequent isotype differentiation to IgE and IgG1 production. PGE2 and PGE1 were found to inhibit cellular enlargement induced by IL-4 or bacterial LPS, IL-4 and LPS, or anti-mu and IL-4 by approximately 75%, and completely inhibit enlargement in response to anti-mu antibody. PGE2 also suppresses activation-induced class II MHC up-regulation by 35% and expression of the low affinity IgE receptor, Fc epsilon RII/CD23, by 30%. Interestingly, PGE completely inhibits a fraction of cells from these activation events, while other cells fully respond to activation stimuli, even in the presence of high PGE2 concentrations. Therefore, a PGE-resistant subset of B lymphocytes may exist. A closely related PG, PGF2 alpha, had no immunoregulatory effect in these systems. Because PGE induces production of cAMP in B cells, we determined whether other agents that increase cAMP could inhibit B cell activation. Cholera toxin and dibutyryl cAMP mimicked the ability of PGE2 to inhibit B cell enlargement, and class II MHC and Fc epsilon RII induction, suggesting that PGE2 signaling occurs via cAMP. In addition, cholera toxin and dibutyryl cAMP inhibited B cell activation much more potently (90-100% inhibition) than PGE, indicating that whereas all B cells are cAMP-sensitive only some are PGE-sensitive. Although PGE inhibits activation-associated events, we previously reported that PGE enhances IL-4 and LPS-induced differentiation to IgE and IgG1 synthesis. To investigate the relationship between the cells that are activation-inhibited and those that are differentiation-enhanced by PGE, we sorted B cell subsets by FACS and determined their relative abilities to produce IgM, IgG1, and IgE in response to IL-4 and LPS in the presence of PGE. The population of lymphocytes that was unaffected by PGE in terms of class II hyperexpression was also unaffected by PGE for Ig synthesis, again indicating a PGE-resistant subpopulation of B cells. Furthermore, the PGE activation-inhibited subset of B cells was responsive to PGE enhancement of IL-4-induced class switching, reducing IgM synthesis and inducing a sevenfold increase in IgE and IgG1 synthesis compared with other sort groups. These results are consistent with the hypothesis that the B lymphocytes that are PGE activation-inhibited are the same cells that are PGE differentiation-enhanced.(ABSTRACT TRUNCATED AT 400 WORDS)
Nanoporous Low Dielectric Cyclosiloxane Bearing Polysilsesquioxane Thin Films Templated by Poly(ε‐caprolactone) (2004)

Abstract Summary: Dielectric cyclosiloxane bearing polysilsesquioxanes (CS‐PSSQs) were prepared by acid catalyzed polymerization using 2,4,6,8‐tetramethyl‐2,4,6,8‐tetra(trimethoxysilylethyl)cyclotetrasiloxane. The molecular weight, and content of the functional end‐groups of the CS‐PSSQs were found to be dependent on the process parameters, such as the molar ratio of water and catalyst to the OCH 3 group of the silane monomer, the amount of solvent, and the poly( ε ‐caprolactone) (PCL) content, etc. Based on these CS‐PSSQ prepolymers, nanoporous organic/inorganic hybrid thin films were fabricated by spin‐coating mixtures of these prepolymers with star‐shaped PCL on the silicon substrate, and subsequently heating them at 150 and 250 °C for 1 minute each and then at 420 °C for 1 hour. A dielectric constant as low as 2.28 was achieved for the nanoporous PCL/CS‐PSSQ (3:7 v/v) film, along with low moisture absorption in ambient and water conditions, primarily due to the presence of hydrophobic moieties such as ethylene and methyl groups of the polymer and nano‐sized hydrophobic pores inside the matrix, resulting in the film having stable dielectric properties. Moreover, the PCL/CS‐PSSQ (3:7 v/v) nanohybrid film revealed a nanoporous structure containing ca. 1.52 nm of average‐sized mesopores, as measured by the N 2 adsorption method. The CS‐PSSQ‐only film showed high mechanical strengths having an elastic modulus and hardness of 6.64 and 0.88 GPa, respectively, for the 7 500 Å thick film and 2.41 and 0.38 GPa, respectively, for the PCL/CS‐PSSQ (3:7 v/v) film. In addition, the crack velocity of the CS‐PSSQ‐only film was found to be ca. 10 −11 m · s −1 in ambient conditions and an aqueous environment, probably due to the enhanced hydrophobicity and mechanical toughness of the incorporated methyl group, siloxane unit and ethylene moieties in the polymer matrix. magnified image
Three-dimensional Printing of Large Nasal Septal Perforations for Optimal Prosthetic Closure (2016)

Sizing done by 3-D printing for prosthetic closure of nasal septal perforations resulted in a higher retention rate in helping patients with these most-challenging nasal septal perforations.
A Battery Lifetime Improved Control Strategy of Modular Multilevel Converter for Electric Vehicle Application (2019)

The application of modular multilevel converter (MMC) in electric vehicle (EV) system is being studied in recent years as one of the emerging research topics, for its various advantages such as high modularity, fault tolerant capability, high quality output and the ability to integrate function of motor drive, on-board charger and cell equalizer. However, specific ac current harmonics introduced by operation of MMC will speed up battery degradation and are harmful to battery lifetime. In past researches on MMC in EV, there is no related study solving this problem. In this paper, an improved control method of MMC based on second order circulating current injection is proposed, mitigating the negative influence of current harmonics on battery lifetime, without increasing hardware cost. Simulation results in MATLAB/Simulink verify the effectiveness of proposed method.
Reversible Linkage of Two Distinct Small Molecule Inhibitors of Myc Generates a Dimeric Inhibitor with Improved Potency That Is Active in Myc Over-Expressing Cancer Cell Lines (2015)

We describe the successful application of a novel approach for generating dimeric Myc inhibitors by modifying and reversibly linking two previously described small molecules. We synthesized two directed libraries of monomers, each comprised of a ligand, a connector, and a bioorthogonal linker element, to identify the optimal dimer configuration required to inhibit Myc. We identified combinations of monomers, termed self-assembling dimeric inhibitors, which displayed synergistic inhibition of Myc-dependent cell growth. We confirmed that these dimeric inhibitors directly bind to Myc blocking its interaction with Max and affect transcription of MYC dependent genes. Control combinations that are unable to form a dimer do not show any synergistic effects in these assays. Collectively, these data validate our new approach to generate more potent and selective inhibitors of Myc by self-assembly from smaller, lower affinity components. This approach provides an opportunity for developing novel therapeutics against Myc and other challenging protein:protein interaction (PPI) target classes.
The effect of displacement current on the measurement of reversal potential in squid giant axon (1982)

The measurement of the sodium reversal potential (Erev), as that potential where the early current reverses during voltage clamp, was found to exceed the true Erev by 4.1 +/- 2.4 mV (mean +/- SD) in squid giant axon. This error was found in both intact and internally perfused axons and is due to interference from the displacement current. This was shown by subtraction of the current records obtained before and after treatment with tetrodotoxin (TTX). The error in Erev is proportional to (Td/gNA)12 where Td is the time constant of the displacement current.
MARKERLESS ANALYSIS OF SWIMMERS’ MOTION: A PILOT STUDY (2009)

Regular laboratory-based motion analysis with skin surface markers is not always feasible. In particular, when studying swimmers kinematics, traditional motion capture techniques cannot be adopted. Although video recordings from swimmers often exist, current methods for biomechanical analysis of these are inadequate. They usually rely on manual digitization of joints’ position on a single sagittal view of the subject. Therefore, in this study a method for three dimensional (3D) markerless motion capture of swimmers is presented. The method adopts the markerless motion capture system developed at Stanford University.
Soy Biodiesel Exhaust is More Toxic than Mineral Diesel Exhaust in Primary Human Airway Epithelial Cells (2019)

As global biodiesel production increases, there are concerns over the potential health impact of exposure to the exhaust, particularly in regard to young children who are at high risk because of their continuing lung development. Using human airway epithelial cells obtained from young children, we compared the effects of exposure to exhaust generated by a diesel engine with Euro V/VI emission controls running on conventional diesel (ultra-low-sulfur mineral diesel, ULSD), soy biodiesel (B100), or a 20% blend of soy biodiesel with diesel (B20). The exhaust output of biodiesel was found to contain significantly more respiratory irritants, including NO<sub><i>x</i></sub>, CO, and CO<sub>2</sub>, and a larger overall particle mass. Exposure to biodiesel exhaust resulted in significantly greater cell death and a greater release of immune mediators compared to both air controls and ULSD exhaust. These results have concerning implications for potential global health impacts, particularly for the pediatric population.
CARRIER PHASE TRACKING AT LOW SIGNAL-TO-NOISE RATIO: A PERFORMANCE COMPARISON OF A PARITY-CODE-AIDED AND A PILOT- SYMBOL-ASSISTED APPROACH (2013)

The last decade has seen the development of powerful channel codes such as turbo codes and low-density parity-check (LDPC) codes. The impressive bit error rate (BER) performance of the associated decoding processes implicitly assumes coherent detection, meaning that the carrier phase must be recovered accurately before the data is decoded. However, since the receiver usually operates at extremely low signal-to-noise ratio (SNR) values, conventional carrier
Polymer coating of porcine decellularized and cross‐linked aortic grafts (2010)

This article investigates a method of modifying and optimizing the biocompatibility of decellularized vascular bioimplants when treated with a specialized, drug eluting coating. For this purpose, we carried out aortic transplantations using a porcine model. Decellularized, cross-linked aortic grafts were coated with poly(D,L-lactide) (PDLLA). To this coating, we added the anticoagulant drug lepirudin which, following transplantation, would be linearly eluted. These aortic grafts are easily manipulated in surgery. It was shown that, as a result of the lepirudin-eluting coating, the rate of thrombogenesis was reduced and the patency rate was significantly improved. However, lumen-stenosing pseudointima developed in all of the transplants and was not effected by PDLLA coating. Furthermore, no evidence of recellularisation was documented. This trial demonstrates that polymer coating of decellularized tissue is possible. Neointimal hyperplasia and the absence of cellular repopulation mark the negative consequences of this concept.
Adaptively secure broadcast, revisited (2011)

We consider the classical problem of synchronous broadcast with dishonest majority, when a public-key infrastructure and digital signatures are available. In a surprising result, Hirt and Zikas (Eurocrypt 2010) recently observed that all existing protocols for this task are insecure against an adaptive adversary who can choose which parties to corrupt as the protocol progresses. Moreover, they prove an impossibility result for adaptively secure broadcast in their setting.We argue that the communication model adopted by Hirt and Zikas is unrealistically pessimistic. We revisit the problem of adaptively secure broadcast in a more natural synchronous model (with rushing), and show that broadcast is possible in this setting for an arbitrary number of corruptions. Our positive result holds under a strong, simulation-based definition in the universal-composability framework.We also study the impact of adaptive attacks on protocols for secure multi-party computation where broadcast is used as a sub-routine.
Erratum: “Density of states simulations of proteins” [J. Chem. Phys. 118, 4285 (2003)] (2003)

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Implementation of a high speed multiport register file in a 1.8 V, 0.25 μm CMOS bulk and SOI technology (2003)

The experimental hardware results of a high speed 8-port, 32 word/spl times/64-bit register file in 1.8 V, 0.25 /spl mu/m CMOS bulk and SOI silicon technology are presented. Such a register file is designed for bulk technology but is also remapped and fabricated in SOI technology without any body contacts. It is shown that the register file in SOI achieves more than 20% performance gain over the counterpart.
Microglial Activation Is Associated With Vasoprotection in a Rat Model of Inflammatory Retinal Vasoregression (2021)

Vascular dysfunction and vasoregression are hallmarks of a variety of inflammatory central nervous system disorders and inflammation-related retinal diseases like diabetic retinopathy. Activation of microglia and the humoral innate immune system are contributing factors. Anti-inflammatory approaches have been proposed as therapies for neurovascular diseases, which include the modulation of microglial activation. The present study aimed at investigating the effects of microglial activation by clodronate-coated liposomes on vasoregression in a model of retinal degeneration. Clodronate treatment over 5 weeks led to an increase in activated CD74<sup>+</sup> microglia and completely prevented acellular capillaries and pericyte loss. Gene expression analyses indicated that vasoprotection was due to the induction of vasoprotective factors such as <i>Egr1</i>, <i>Stat3</i>, and <i>Ahr</i> while expression of pro-inflammatory genes remained unchanged. We concluded that activated microglia led to a shift toward induction of pleiotropic protective pathways supporting vasoprotection in neurovascular retinal diseases.
Inventory of telomerase components in human cells reveals multiple subpopulations of hTR and hTERT (2014)

Telomerase is the ribonucleoprotein (RNP) enzyme that elongates telomeric DNA to compensate for the attrition occurring during each cycle of DNA replication. Knowing the levels of telomerase in continuously dividing cells is important for understanding how much telomerase is required for cell immortality. In this study, we measured the endogenous levels of the human telomerase RNP and its two key components, human telomerase RNA (hTR) and human telomerase reverse transcriptase (hTERT). We estimate ∼ 240 telomerase monomers per cell for HEK 293T and HeLa, a number similar to that of telomeres in late S phase. The subunits were in excess of RNPs (e.g. ∼ 1150 hTR and ∼ 500 hTERT molecules per HeLa cell), suggesting the existence of unassembled components. This hypothesis was tested by overexpressing individual subunits, which increased total telomerase activity as measured by the direct enzyme assay. Thus, there are subpopulations of both hTR and hTERT not assembled into telomerase but capable of being recruited. We also determined the specific activity of endogenous telomerase and of overexpressed super-telomerase both to be ∼ 60 nt incorporated per telomerase per minute, with Km(dGTP) ∼ 17 μM, indicating super-telomerase is as catalytically active as endogenous telomerase and is thus a good model for biochemical studies.
Characterization of the Cellular Activity of PDE 4 Inhibitors Using Two Novel PDE 4 Reporter Cell Lines (2013)

We report here the generation and pharmacological characterization of two novel PDE 4B1 and PDE 4D3 reporter cell lines. Intracellular cAMP levels are monitored in these cells by a cAMP-sensitive biosensor. We used the recombinant PDE 4B1 and PDE 4D3 reporter cell lines to characterize the cellular effects of various competitive and allosteric PDE 4 inhibitors. In addition, we compared the cellular activity of these PDE 4 inhibitors with the in vitro inhibition of full-length PDE 4D3 and a truncated enzyme comprising the PDE 4D3 catalytic domain. Two different groups of PDE 4 inhibitors could be identified. The first group, including competitive inhibitors like roflumilast, cilomilast and piclamilast, shows similar in vitro activity on full-length and truncated PDE 4D3 and comparably low cellular activity. The second group, including the allosteric inhibitors PMNPQ, D159153, and D159404, shows much better inhibition of full-length versus truncated PDE 4D3. In addition, these compounds show high cellular activity. Our data obtained with the prototype PDE 4 inhibitor rolipram show that rolipram has properties intermediate between the two groups. The results imply that these novel PDE 4 reporter cell lines are well-suited for the characterization of the cellular activity of PDE 4 inhibitors and may also support a better understanding of the complex PDE 4 pharmacology.
Kinetics of Triglyceride Solubilization by Micellar Solutions of Nonionic Surfactant and Triblock Copolymer. 2. Theoretical Model (2002)

A theoretical model of oil solubilization in micellar surfactant solutions is developed. We consider oils that are practically insoluble in pure water, like triolein and other triglycerides. The nonionic micelles, which are capable to solubilize such oils, are usually rodlike aggregates, composed of surfactant molecules and poly(oxyethylene)−poly(oxypropylene) triblock copolymers, like Synperonic L61 (SL61). The swollen micelles, formed after solubilization, are smaller than the empty ones. The model describes the elementary act of solubilization as a sequence of three steps: (a) adsorption of an empty micelle at the oil−water interface; (b) uptake of oil by the adsorbed empty micelle which then splits into several swollen micelles; (c) desorption of the swollen micelles. Theoretical expressions are derived, which describe the diminishing of an oil drop in the course of solubilization. The parameter values, determined from the best fit of experimental data, imply that the rate-controlling step is the step of micellar adsorption. From the determined rate constant of adsorption we estimate the respective kinetic barrier to micelle adsorption, taking into account the action of surface forces and the hydrodynamic resistance. Our analysis indicates that the triblock copolymer SL61 promotes the solubilization of triglycerides by decreasing the length of the mixed surfactant−copolymer rodlike micelles, which leads to a lowering of the kinetic barrier to their adsorption at the oil−water interface.
A review of alternative intravenous acetylcysteine regimens for acetaminophen overdose (2021)

Several alternative regimens have been studied and implemented in various institutions. Many of these dosing regimens have supporting safety data but most lack robust data. A reduction in infusion-related side effects is an important outcome, but established efficacy, local poison center familiarity with the regimen, institutional resources, and patient-specific factors should be equally considered when deciding on implementing and using an alternative dosing strategy.
The Role of Modified Graphene in Cathode Formulations for Lithium-Ion Batteries (2015)

We explored the effect of reduced graphene oxide (RGO) on LiNi 0.5 Mn 1.5 O 4 (LNMO) and on 0.6LiMnPO 4 –0.4Li 3 V 2 (PO 4 ) 3 (LMVP) composite cathodes with carbon black (C65). We demonstrated that the effect of RGO depends on the cathode active material. Indeed, RGO improves cycling stability of LNMO cathodes by improving electrode/electrolyte interface stability at high potentials. On the contrary, RGO worsens the performance of LMVP/C65 and it has no effect on LMVP electrodes when it is produced in situ during LMVP synthesis in presence of graphene oxide. Our study indicates that at least 10 wt. % of C65 is necessary for good performance of the poor conductive LMVP.
Molecular Modeling in Drug Design (2003)

Abstract This chapter examines the approaches to molecular modeling and drug design and emphasizes their limitations. Useful information to guide the design and synthesis of potential novel therapeutics can be developed from an analysis of structure‐activity data in the three‐dimensional framework provided by current molecular modeling techniques. Although most of the techniques and approaches described have broader application than shown, the examples chosen should be sufficient to illustrate their use.
Element-Selective Single Atom Imaging (2000)

Electron energy-loss spectroscopy (EELS) is widely used to identify elemental compositions of materials studied by microscopy. We demonstrate that the sensitivity and spatial resolution of EELS can be extended to the single-atom limit. A chemical map for gadolinium (Gd) clearly reveals the distribution of Gd atoms inside a single chain of metallofullerene molecules (Gd@C82) generated within a single-wall carbon nanotube. This characterization technique thus provides the "eyes" to see and identify individual atoms in nanostructures. It is likely to find broad application in nanoscale science and technology research.
Characterization of bilateral, pudendal nerve-evoked, urethral rhabdosphincter contractions in anesthetized cats (2022)

Pudendal (PUD) efferent activity contracts urethral rhabdosphincters to resist urine leakage. This study evaluates the potential for PUD nerve stimulation to provide benefit for urinary incontinence. Acute responses of the urethral rhabdosphincter to modulation of current amplitude, pulse duration (PD), and frequency of stimulation were examined. Urethral pressure was measured using a microtip catheter in chloralose-anesthetized, male cats with or without acute spinalization at T10. Stimulation was applied from an external pulse generator coupled to cuff electrodes placed around left and right PUD nerves in the ischiorectal fossa, central to its branching. Bladder pressure clamp techniques were used for testing effects of stimulation on time and rate of leakage during PUD stimulation. Single pulses (0.1 ms) produced single transient increases in urethral pressure that peaked at 50 ms and diminished to 50% of peak at 90 ms. Threshold current was 88 ± 30μA with maximal responses occurring with 50% increases in current. Peak pressures increased with increasing PDs between 40–180μs. Summation, tetany, peak pressures, and fatigue of responses to increasing stimulation frequencies during 2 min periods of stimulation are described. Stimulation under pressures that are typical during bladder filling or during bladder contractions (30–40 cm H2O) prevented leakage up to an hour in some animals, increased the median time to leak from 0.3 min to 56.0 min, and decreased the median rate of leakage from 1.44 ml/min to 0.04 ml/min when leakage did occur in less than hour. During rapid elevations in bladder pressure to simulate a Valsalva-like bladder pressure (120 cm H2O), PUD stimulation increased the median time to leak from 9 s to 93 s and decreased the median rate of leakage from 5.5 ml/min to 3.4 ml/min. These results support future studies of bilateral PUD stimulation in a clinically-relevant, animal model of urinary incontinence.