gebhart/patrepeval huggingface dataset:
Integrating microfluidics and biosensing on a single flexible acoustic device using hybrid modes (2020)

Integration of microfluidics and biosensing functionalities on a single device holds promise in continuous health monitoring and disease diagnosis for point-of-care applications. However, the required functions of fluid handling and biomolecular sensing usually arise from different actuation mechanisms. In this work, we demonstrate that a single acoustofluidic device, based on a flexible thin film platform, is able to generate hybrid wave modes, which can be used for fluidic actuation (Lamb waves) and biosensing (thickness shear waves). On this integrated platform, we show multiple and sequential functions of mixing, transport and disposal of liquid volumes using Lamb waves, whilst the thickness bulk shear waves allow us to sense the chemotherapeutic Imatinib, using an aptamer-based strategy, as would be required for therapy monitoring. Upon binding, the conformation of the aptamer results in a change in coupled mass, which has been detected. This platform architecture has the potential to generate a wide range of simple sample-to-answer biosensing acoustofluidic devices.
Herpes simplex virus vector-mediated expression of Bcl-2 prevents 6-hydroxydopamine-induced degeneration of neurons in the substantia nigra in vivo (1999)

6-Hydroxydopamine (6-OHDA) is widely used to selectively lesion dopaminergic neurons of the substantia nigra (SN) in the creation of animal models of Parkinson's disease. In vitro, the death of PC-12 cells caused by exposure to 6-OHDA occurs with characteristics consistent with an apoptotic mechanism of cell death. To test the hypothesis that apoptotic pathways are involved in the death of dopaminergic neurons of the SN caused by 6-OHDA, we created a replication-defective genomic herpes simplex virus-based vector containing the coding sequence for the antiapoptotic peptide Bcl-2 under the transcriptional control of the simian cytomegalovirus immediate early promoter. Transfection of primary cortical neurons in culture with the Bcl-2-producing vector protected those cells from naturally occurring cell death over 3 weeks. Injection of the Bcl-2-expressing vector into SN of rats 1 week before injection of 6-OHDA into the ipsilateral striatum increased the survival of neurons in the SN, detected either by retrograde labeling of those cells with fluorogold or by tyrosine hydroxylase immunocytochemistry, by 50%. These results, demonstrating that death of nigral neurons induced by 6-OHDA lesioning may be blocked by the expression of Bcl-2, are consistent with the notion that cell death in this model system is at least in part apoptotic in nature and suggest that a Bcl-2-expressing vector may have therapeutic potential in the treatment of Parkinson's disease.
The Novel Antiobesic HMR1426 Reduces Food Intake without Affecting Energy Expenditure in Rats (2004)

HMR1426 showed an anorectic potential in rats and decreased body weight and fat mass. This was achieved solely by reducing food intake without influencing overall energy expenditure or behavior suggesting a peripheral mode of action. Thus, HMR1426 can be considered a potential new drug for obesity treatment.
Multi-Band Outdoor-to-Indoor Propagation Measurements Using a Drone (2022)

Outdoor-to-indoor propagation path-loss measurements have been carried out at 27 and 38 GHz, two of the frequencies allocated for 5G networks, for two different buildings: an office glass, steel and concrete building and a brick-wall residential house. The outdoor station has been mounted on a drone in order to have more placement flexibility and reach the desired height above ground without the use of a crane truck. Overall outdoor-to-indoor loss seems to depend primarily on the window surface and on the construction material. While loss is higher at 38GHz with respect to 27 GHz for the office building, brick-walls of the residential house appear to be less frequency selective.
Birefringence phantoms for polarization sensitive optical coherence tomography (Conference Presentation) (2017)

Polarization sensitive optical coherence tomography (PS-OCT) is increasingly used in a range of applications, both in bench-top and catheter-based imaging configurations. Reconstruction of tissue birefringence is subject to many system and processing-dependent artifacts. However, methods for the calibration and validation of PS-OCT are missing. Here, we report on a method to fabricate tissue-like imaging phantoms exhibiting clearly defined regions with controllable amounts of birefringence. We employed the photoelastic effect to enable the generation of controllable amounts of stress-induced birefringence in rubber samples, verified with polarized light microscopy. Pigmented ink was added to liquid latex solution to mold and cure rubber bands with controlled backscattering and transparency. Differently stretched segments were embedded in a stress-free background matrix to generate clearly defined areas with high birefringence contrast in an area of homogenous backscatter intensity. Arranged in planar geometry or on the outside of a glass capillary, the stretched rubber bands defined phantoms for bench-top and catheter-based imaging, respectively. Segmentation of the defined regions of interest in the reconstructed volumetric birefringence tomograms enabled assessing measurement consistency, between repeated imaging with a single system, or between independent imaging systems. Consistent and durable imaging phantoms are crucial for advancing PS-OCT imaging technology. Our tissue-like imaging phantoms exhibit clearly defined regions with controlled amounts of birefringence and facilitate testing, calibration, and validation of imaging systems and reconstruction strategies.
Batch-Screening Guided Continuous Flow Synthesis of the Metal-organic Framework HKUST-1 in a Millifuidic Droplet Reactor (2022)

Metal-organic frameworks (MOFs) are a class of crystalline and porous adsorbents, with wide-ranging applications in gas separations, membrane materials as well as sensors. Commonly used batch synthesis techniques for MOF production are limited by low productivity, high operating costs, and slow crystallization timescales, severely impeding the large-scale manufacturing of these materials. However, batch synthesis is a useful and easy technique to screen multiple reaction parameters to find an optimal chemistry. Therefore, in this study, we have used the batch process and screened a multidimensional reaction space consisting of 45 sample variations based on the crystallinity, yield and instantaneous precipitation, which could lead to tube clogging under flow conditions. We have found one optimized reaction chemistry, that could be used in flow conditions, which in this study is a novel millifluidic droplet-based reactor for the continuous synthesis of HKUST-1 crystals. The biphasic flow in the millifluidic reactor consisted of droplets of the reactant solution, dispersed in a continuous phase of silicone oil. We investigate the differences in the quality and quantity of HKUST-1 synthesized via the continuous and batch techniques. Moreover, we have demonstrated that the HKUST-1 samples prepared via the continuous synthesis in a droplet based millifluidic reactor, at an ultra-low residence time exhibit excellent physical properties comparable to that obtained for the samples prepared by the traditional batch process. A clean, easy-to-install, and reusable millifluidic reactor presented in this work may pave the path for an economically viable, large-scale synthesis of HKUST-1.
Control of immune-mediated pathology via the aryl hydrocarbon receptor (2017)

Genetic and environmental factors contribute to the development of immune-mediated diseases. Although numerous genetic factors contributing to autoimmunity have been identified in recent years, our knowledge on environmental factors contributing to the pathogenesis of autoimmune diseases and the mechanisms involved is still limited. In this context, the diet, microbiome, geographical location, as well as environmental pollutants have been shown to modulate autoimmune disease development. These environmental factors interact with cellular components of the immune system in distinct and defined ways and can influence immune responses at the transcriptional and protein level. Moreover, endogenous metabolites generated from basic cellular processes such as glycolysis and oxidative phosphorylation also contribute to the shaping of the immune response. In this minireview, we highlight recent progress in our understanding of the modulation of the immune response by the aryl hydrocarbon receptor (AhR), a ligand-activated transcription factor whose activity is regulated by small molecules provided by diet, commensal flora, environmental pollutants, and metabolism. We focus on the role of AhR in integrating signals from the diet and the intestinal flora to modulate ongoing inflammation in the central nervous system, and we also discuss the potential therapeutic value of AhR agonists for multiple sclerosis and other autoimmune diseases.
[Composition and Evolution Characteristics of Dissolved Organic Matter During Composting Process]. (2016)

According to the fraction method presented by Leenheer, dissolved organic matter (DOM) extracted from chicken manure with different composting time was fractionated into five groups [i. e., hydrophobic acid (HOA), hydrophobic base (HOB), hydrophobic neutral (HON) fractions, acid-insoluble (AIM) and hydrophilic matter (HIM)] using the XAD-8 resin based on its polarity and electric charge characteristics. The composition and structures of these fractions were investigated by elemental analysis, FTIR and <sup>1</sup>H-NMR spectra. The results showed that the HIM and HOA fractions accounted for 32%-44% and 35%-47% of DOM, respectively, during the composting process, while the sum of the fractions HOB, AIM and HON was responsible for less than 25% of DOM. The HIM content decreased while the hydrophobic component increased after composting. The elemental analysis indicated that, during the composting process, the N, C, S content of all five fractions increased, the H/C ratio decreased, but the humification degree increased; The result from <sup>1</sup>H-NMR analysis showed that the HIM had low alkyl chain, short branched chain, high branches and carbohydrate structure, which was opposed to the HON fraction. The composition of AIM was similar to that of humic acids, while the HOB was rich in nitrogen-containing compounds. The result from the FTIR analysis showed that the HOA and HOB fractions were rich in abundant carboxyl, ester group and hydroxyl functional group. Besides the above functional groups, the AIM and HIM were rich in benzene groups. The HON fraction was rich in aliphatic functional group except for the abovementioned functional groups. The aliphatic functional group was degraded and the benzene functional group after composting.
Wireless Bioelectronic Nanosystems for Intracellular Communication (2018)

In order for the field of bioelectronics to make an impact on healthcare, there is an urgent requirement for the development of “wireless” electronic systems to both sense and actuate cell behaviour. Herein we report the first example of an innovative intracellular wireless electronic communication system. We demonstrate that chemistry can be electrically modulated in a “wireless” manner on the nanoscale at the surface of conductive nanoparticles uptaken by cells at unreported low potentials. The system is made functional by modifying gold nanoparticles incorporating a Zn-porphyrin, which are taken up by cells and are shown to be biocompatible. It is demonstrated the redox state of Zn-porphyrin modified gold nanoparticles is modulated and reported on fluorescently when applying an external electrical potential. This provides an attractive new “wireless” approach to develop novel bioelectronic devices for modulating and sensing cellular behaviour using intracellular monitoring.
Unmet Needs and Evolving Treatment for Limb Girdle Muscular Dystrophies (2021)

Limb-girdle muscular dystrophies (LGMDs) represent a major group of muscle disorders. Treatment is sorely needed and currently expanding based on safety and efficacy adopting principles of single-dosing gene therapy for monogenic autosomal recessive disorders. Gene therapy has made in-roads for LGMD and this review describes progress that has been achieved for these conditions. This review first provides a background on the definition and classification of LGMDs. The major effort focuses on progress in LGMD gene therapy, from experimental studies to clinical trials. The disorders discussed include the LGMDs where the most work has been done including calpainopathies (LGMD2A/R1), dysferlinopathies (LGMD2B/R2) and sarcoglycanopathies (LGMD2C/R5, LGMD2D/R3, LGMD2E/R4). Early success in clinical trials provides a template to move the field forward and potentially apply emerging technology like CRISPR/Cas9 that may enhance the scope and efficacy of gene therapy applied to patient care.
3D Wide-Angle Impedance Matching for X-Band Phased Array (2021)

The design of 3D Wide-angle impedance matching is presented in this paper. The design of an X-band array element, further illustrating the use of 3D WAIMs, is first detailed. This X-band element presents limited scanning performance which is improved thanks to the addition of WAIMs. The need of 3D WAIMs is justified through an analytical model. Finally, the antenna design is presented with the optimized 3D WAIM placed above the X-band element. The improvements in scanning performance are analysed, through the active reflection coefficient and radiation patterns.
Structural characterization and antimicrobial activity of a silver(I) complex of arginine (2015)

A silver(I) complex of arginine (arg), [Ag(arg)]NO3•0.5H2O (1) was prepared and characterized by elemental analysis, IR and NMR spectroscopy, and X-ray crystallography. The IR and NMR spectroscopic data confirmed the coordination of the ligand to silver(I). The structure of 1 shows that it is polymeric with each silver atom bound to the carboxyl O atom of one arginine ligand and to the amino N atom of another adopting a linear coordination environment. The two coordinate N–Ag–O units are repeated to form infinite chains. The molecular structure is stabilized by N–H…O and C–H…O hydrogen bonds. Antimicrobial activities of the complex were evaluated by minimum inhibitory concentration against gram-negative bacteria (E. coli, P. aeruginosa), molds (A. niger, P. citrinum) and yeasts (C. albicans, S. cerevisiae).
Lovable couch (2015)

Increasing percentage of unmarried individuals in Japan has triggered decline in birth rate. This is partially caused by dominating modern lifestyles that involve long working hours, as well as increasing sex segregation in social interaction. People who are singles have fewer opportunities to build romantic relationships; therefore, speed-dating services have recently become popular. However, challenges still remain in supporting dating interaction especially to determine whether a potential couple feels affection toward each other. Hence, many people feel distrust and anxiety when being approached by a dating partner thus makes them feel hesitate to move forward. In this work, we report our findings that by visualizing excitation aggregated from users' heartbeat changes potentially help users to determine whether their potential partners feel mutual affection during a date. We propose Lovable Couch, an approach to support dating session by visually actuating a sofa with user's excitement measures as a way to mitigate users' anxiety.
Conjugational transfer of recombinant DNA in cultures and in soils: host range of Pseudomonas putida TOL plasmids (1991)

Recombinant TOL plasmid pWWO-EB62 allows Pseudomonas putida to grow on p-ethylbenzoate. This plasmid can be transferred to other microorganisms, and its catabolic functions for the metabolism of alkylbenzoates are expressed in a limited number of gram-negative bacteria, including members of pseudomonad rRNA group I and Escherichia coli. Transfer of the recombinant plasmid to Erwinia chrysanthemi was observed, but transconjugants failed to grow on alkylbenzoates because they lost catabolic functions. Pseudomonads belonging to rRNA groups II, III, and IV, Acinetobacter calcoaceticus, and Alcaligenes sp. could not act as recipients for TOL, either because the plasmid was not transferred or because it was not stably maintained. The frequency of transfer of pWWO-EB62 from P. putida as a donor to pseudomonads belonging to rRNA group I was on the order of 1 to 10(-2) transconjugant per recipient, while the frequency of intergeneric transfer ranged from 10(-3) to 10(-7) transconjugant per recipient. The profile of potential hosts was conserved when the donor bacterium was Escherichia coli or Erwinia chrysanthemi instead of P. putida. No intergeneric gene transfer of the recombinant TOL plasmid was observed in soils; however, intraspecies transfer did take place. Intraspecies transfer of TOL in soils was affected by the type of soil used, the initial inoculum size, and the presence of chemicals that could affect the survival of the donor or recipient bacteria.
Expression of intracellular filament, collagen, and collagenase genes in diabetic and normal skin after injury (2006)

Reports have shown differences in gene expression in the skin of diabetic and normal mice both at baseline and after injury. Cluster analysis identified distinct expression patterns within intermediate filaments and extracellular proteins. This report addresses the effect of diabetes and injury on the expression of keratin-associated proteins, keratin complexes, procollagen, and collagenase (matrix metalloproteinase; MMP) genes. At baseline keratin-associated proteins and keratin complexes gene expression was increased in diabetic mice. After surgery, the level of expression for keratin-associated proteins and keratin complexes genes decreased in diabetic mice, but did not change in normal mice. If the expression of a procollagen gene differed between diabetic and normal mice, the expression was lower in diabetic mice. Procollagen gene expression was elevated after skin excision compared with noninjured skin. At baseline, the level of MMP and tissue inhibitor of metalloproteinase gene expression was comparable between mouse strains. With injury, the expression of several MMP genes was increased in both mouse strains, but to higher levels in diabetic mice. At day 7, the level of MMP-9 activity in granulation tissue was elevated. This alteration may contribute to delayed healing in diabetic mice. Therefore, differences in gene expression exist between mouse strains and can assist in understanding of physiological manifestations, including delayed healing, in diabetic mice.
Catalysis by framework zinc in silica-based molecular sieves (2016)

Microporous and mesoporous zincosilicates (<i>e.g.</i>, CIT-6, VPI-8, Zn-MFI, and Zn-MCM-41) synthesized in the presence of alkali cations contain two broad types of Zn sites: one that is a dication analog of the monocation ion-exchangeable Al-site in aluminosilicates, while the other resembles isolated Zn sites on amorphous silica. The ratio of these sites varies, depending on the synthesis conditions of the zincosilicate. Post-synthetic strategies based on ion-exchange can alter the site distribution towards either population. Furthermore, post-synthetic introduction of isolated Zn sites of the latter type is possible for materials possessing silanol nests. Both types of sites behave as Lewis acid centers in probe-molecule IR spectroscopy, but have very different catalytic properties. Due to the unusually high adsorption energies of Lewis bases on such materials, Lewis acid catalysis is difficult at low temperatures and in solvents bearing Lewis basic functionality. However, at high temperatures, in hydrocarbon solvents, CIT-6 (Zn-beta) is able to selectively catalyze the Lewis-acid-catalyzed Diels-Alder cycloaddition-dehydration reactions of ethylene with methyl 5-(methoxymethyl)furan-2-carboxylate, a furan that can be derived quantitatively by partial oxidation of biomass-based 5-hydroxymethylfurfural. Additionally, zinc in silica-based molecular sieves is shown here to enable chemistries previously not accessible with framework Sn-, Ti- and Zr-based Lewis acid sites, <i>e.g.</i>, the direct production of dimethyl terephthalate by Diels-Alder cycloaddition-dehydration reactions of ethylene and the dimethyl ester of furan-2,5-dicarboxilic acid.
Artificially Intelligent Nanoarray for the Detection of Preeclampsia under Real‐World Clinical Conditions (2016)

Artificially intelligent nanoarray based on molecularly-modified gold nanoparticles is tailored for the detection of preeclampsia by noninvasive breathprint analysis. The diagnostic power of the nanoarray under actual clinical conditions shows discrimination between preeclampsia and nonpreeclamptic pregnancy with an 84% accuracy, and between preeclampsia and nonpregnant women with 87% accuracy. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Enzymatic preparation of optically active bicyclo[2.2.1]heptene derivatives, building blocks for terpenoid natural products. An attractive alternative to enantioselective Diels–Alder syntheses (1989)

Bicyclo[2.2.1]heptene derivatives (1)–(3), building blocks for terpenoid natural products, have been prepared with high enantiometric purities by enzymatic hydrolysis of their racemic esters in the presence of porcine liver esterase (PLE) and an ester hydrolase from Pseudomonas sp. (SAM-II).
Recent Advances in BioMedical Telemetry (2007)

Communication systems for implantable medical devices are rapidly advancing. Cardiac, optical, neurological and auditory devices all utilize this technology. Miniaturized antennas and inductive coupling systems provide the radio interface between air and the implantable device. Miniaturized electronics that can be seamlessly integrated with the medical device provide electrical and communication interfaces. Power transfer ("link budget") and deposition (SAR) issues continue to be explored and better understood. This paper describes advances in each of these system components. The challenges of miniature biocompatible packaging, patient variability, and utilization of new materials was discussed.
ChemInform Abstract: Efficient Pd‐Catalyzed Amination Reactions for Heterocycle Functionalization. (2011)

Abstract The method reported in the previous paper is extended to halogenated indazoles, benzimidazoles, benzotriazoles and indoles.