gebhart/patrepeval huggingface dataset:
Use of novel chemical supplements in the establishment of three human malignant lymphoma cell lines (NU‐DHL‐1, NUDUL‐1, and NU‐AMB‐1) with chromosome 14 translocations (1985)

Three new cell lines have been established from patients with malignant lymphoma utilizing a human diploid feeder layer, pooled human serum, and the chemical supplements L-cysteine, iron-saturated transferrin, and bathocuproine disulfonate, a copper chelator. After a short period of growth, the 3 cell lines were successfully weaned from the feeder layers but continued to require human serum and the chemical supplements for up to 9 months of culture. The cell lines are currently grown in RPM1-1640 medium and fetal calf serum without further supplementation. The NU-DHL-1 cell line was established from the involved lymph node of a 73-year-old White male with diffuse large-cell lymphoma. The cell line expresses cytoplasmic IgM/lambda heavy and light chains, is Epstein-Barr virus (EBV)-negative, and is positive for several B-cell markers, indicating that it is derived from a mature-B-cell neoplasm. The NU-DUL-1 cell line was established from the cerebrospinal fluid of a 42-year-old White male with undifferentiated lymphoma, non-Burkitt's type, who initially presented with a mediastinal mass and had subsequent involvement of the central nervous system. The cell line is EBV-negative, but surprisingly it is positive for early B-cell markers. The NU-AmB-1 cell line was established from the abdominal mass of a 12-year-old Hispanic male with undifferentiated lymphoma, Burkitt's type. The cell line is EBV-positive and expresses early B-cell markers. All 3 cell lines are aneuploid or pseudodiploid and contain chromosome 14q+ abnormalities including a newly described complex translocation t(?;1;8;14) in the NU-AmB-1 cell line. The establishment of these cell lines was made possible by refinements in the cell culture of the human malignant lymphomas. The availability of well-characterized lymphoma cell lines with specific chromosomal translocations will aid molecular and cellular studies designed to identify the biological significance of genomic rearrangements.
Novel synthesis of prostaglandin-E2 involving regioselective ring opening of a 2,3-endo-epoxybicyclo[3.2.0]heptan-6-one acetal with a mixed organocuprate reagent (1978)

The epoxyacetal (2) is ring opened by appropriate organometallic reagents regioselectively to afford the acetals (15) and (17) in high yield; the acetal (15) was transformed into the prostaglandin-F2α precursor (4) in two steps, while (17) furnished the prostaglandin-E2 intermediate (24) in three steps.
GEM-type detectors using LIGA and etchable glass technologies (2002)

Gas electron multipliers (GEMS) have been made by a deep X-ray lithography technique (LIGA process) using synchrotron radiation on polymethylmethacrylate (PMMA) and by ultraviolet (UV) processes using a UV etchable glass. The gain, stability, and rate capability for these detectors are described. The LIGA detectors described consist of PMMA sheets of various thicknesses, 125-350 /spl mu/m, and have 150/spl times/150 /spl mu/m/sup 2/ holes spaced with a pitch of 300 /spl mu/m. Thin copper electrodes are plated on the top and bottom surfaces using a Damascene method, followed by electroless plating of the copper onto a palladium-tin-base layer. For various thicknesses of PMMA, measurements have been made of absolute gain versus voltage, time stability of gain, and rate capability. The operating gas mixture was usually Ar/CO/sub 2/ (70/30) gas, but some tests were also done using P10 gas. We also made GEM-like detectors using the UV etchable glass called Foturan, patterned by exposure to UV light and subsequent etching. A few measurements using these detectors will be reported, including avalanche gain and time stability.
Fire ant IPM (2008)

Imported fire ants (Solenopsis invicta, S. richteri and their hybrid) are notorious invasive ants from South America that continue to plague the southern USA since their inadvertent introductions prior to the mid-1930s. They now infest over 129.5 million hectares in the USA. The red imported fire ant (S. invicta), commonly referred to as “fire ant,” has continued to spread and is now a worldwide concern with infestations confirmed in Australia, Southeast Asia and Mexico. The painful, burning sensation that is inflicted by the sting of a fire ant is easily the most recognizable hazard to humans. While one sting is painful, it is not uncommon for a person to receive numerous stings simultaneously when ants swarm out of their nest to attack an intruder. This greatly intensifies the pain and can cause panic; thus fear or apprehension of these ants can be present in heavily infested or newly infested areas. In addition, it is conservatively estimated that 1% of stung individuals in the USA are allergic to the venom and at risk for anaphylaxis. Deaths from fire ant stings have been reported and lawsuits have resulted in awards of over $US 1 million.
Sünbülzâde Vehbî Efendi Divanı’nda Sâkî ve Sâkînin Görüntü Düzeyleri (2017)

Klasik Türk şiirinde kadeh sunması ile öne çıkan sâkî, eğlence meclislerinin en önemli kişisidir. Meclisler, çeşitli mekânlarda kurulan eğlence ortamlarıdır. Bu eğlence meclisleri, evlerde, meyhanelerde, saraylarda, sahralarda tertip edilirdi. Eğlence etkinliklerinde içki dışında sâzendeler, gazelhânlar, rakkaseler ve çeşitli musikî unsurlar bulunurdu. Ayrıca meclisi renklendiren mumlar, buhurdanlıklar ve tütsüler gibi unsurlar da dikkat çekiciydi. Sünbülzâde Vehbî Efendi divanında eğlence meclislerinin aktörü olan sâkînin çeşitli görüntü düzeyleriyle karşılaşılmaktadır. Sâkî mecliste içki sunan özelliği yanında sevgili anlamında da kullanılır. Ayrıca sâkî meclis düzenini sağlar, âşıklara neşe ve huzur verme görevini icra eder. Bu çalışmamızda, III. Selim döneminin Sultanu’ş-Şu’arası (şairler sultanı) unvanıyla anılan XVIII. yüzyıl şairlerinden Sünbülzâde Vehbî Efendi’nin divanında geçen “sâkî” tipi üzerinde durulmuştur. Meclisin baş aktörü olan sâkî, çeşitli yönleriyle tanıtılmıştır. Bu bağlamda sâkînin güzellik unsurları, görevleri ve sâkîye benzetilen unsurlar hakkında bilgi verilmiştir.
Off-the-shelf, steroid-resistant, IL13Rα2-specific CAR T cells for treatment of glioblastoma (2022)

This first-in-human experience establishes a foundation for future adoptive therapy studies using off-the-shelf, zinc-finger modified, and/or glucocorticoid resistant CAR T cells.
Going beyond the display (2008)

We introduce a new type of interactive surface technology based on a switchable projection screen which can be made diffuse or clear under electronic control. The screen can be continuously switched between these two states so quickly that the change is imperceptible to the human eye. It is then possible to rear-project what is perceived as a stable image onto the display surface, when the screen is in fact transparent for half the time. The clear periods may be used to project a second, different image through the display onto objects held above the surface. At the same time, a camera mounted behind the screen can see out into the environment. We explore some of the possibilities this type of screen technology affords, allowing surface computing interactions to extend 'beyond the display'. We present a single self-contained system that combines these off-screen interactions with more typical multi-touch and tangible surface interactions. We describe the technical challenges in realizing our system, with the aim of allowing others to experiment with these new forms of interactive surfaces.
Frequency and amplitude discrimination along the kinesthetic-cutaneous continuum in the presence of masking stimuli (2006)

Frequency and amplitude discrimination thresholds along the kinesthetic to cutaneous continuum were evaluated on the left index fingerpad using a multifinger tactual display. Target stimuli were presented either in isolation (no-masker condition) or in the presence of masking stimuli (one- or two-masker conditions). Six reference target signals in the frequency range 2-300 Hz (two each from low-, medium-, and high-frequency regions) and at an amplitude of either 20 or 35 dB sensation levels (SL) were used. In the no-masker condition, the range of frequency Weber fraction was 0.13-0.38 and 0.14-0.28, and the range of amplitude discrimination threshold was 1.82-2.98 dB and 1.65-2.71 dB, at 20 and 35 dB SL, respectively. In the masking conditions, average frequency Weber fractions rose to 0.60 and 0.46, and average amplitude thresholds rose to 3.63 and 3.72 dB, at 20 and 35 dB SL, respectively. In general, thresholds were largest in the two-masker condition and lowest in the no-masker condition. Although the frequency and amplitude thresholds generally increased in the presence of masking stimuli, there was some indication of channel independence for low- and high-frequency target stimuli. The implications of the results for tactual communication of speech are discussed.
Colocalization of the rat homolog of the von Hippel Lindau (Vhl) gene and the plasma membrane Ca<sup>++</sup> transporting ATPase isoform 2 (<i>Atp2b2</i>) gene to rat chromosome bands 4q41.3→42.1 (1995)

Using fluorescence in situ hybridization, we localized the rat homolog of the von Hippel-Lindau gene (Vhl) to rat chromosome band 4q41.3-->q42.1. We also mapped the gene encoding the plasma membrane Ca(++)-transporting ATPase isoform 2(Atp2b2) to the same chromosome subregion. These two genes together with Raf1 appear to be members of a large syntenic gene cluster that maps to human chromosome bands 3p25-->p26, mouse chromosome bands 6 C3-->E, and rat chromosome bands 4q41-->q42. Cytogenetic analysis of NRK 52E cells derived from immortalized normal rat kidney epithelial cells revealed an inverted duplication of the region containing this gene cluster.
Shot-to-shot comparison of the first Li-beam-driven hohlraum experiments (1995)

Shot-to-shot comparison of the first Li-beam hohlraum experiments on PBFA II provides an opportunity to measure and determine the factor that affect the on-axis reproducibility of the Li-ion beam. Both inner-shell and thermal x-ray emission are used to estimate the relative beam power on target from shot to shot. The M- and L-shell Au x-ray yield measurements were made with an array of 11 filtered p-i-n detectors, and the thermal emission measurements were made with an array of three filtered bolometers. The analysis shows that the beam intensity on target varied by more than a factor of 10 during the shot series. By correlating the results of the beam on target with other diagnostics and events, we have identified some potential causes for these variations.
Resonant x-ray scattering study of the antiferroelectric and ferrielectric phases in liquid crystal devices (2001)

Resonant x-ray scattering has been used to investigate the interlayer ordering of the antiferroelectric and ferrielectric smectic C* subphases in a device geometry. The liquid crystalline materials studied contain a selenium atom and the experiments were carried out at the selenium K edge allowing x-ray transmission through glass. The resonant scattering peaks associated with the antiferroelectric phase were observed in two devices containing different materials. It was observed that the electric-field-induced antiferroelectric to ferroelectric transition coincides with the chevron to bookshelf transition in one of the devices. Observation of the splitting of the antiferroelectric resonant peaks as a function of applied field also confirmed that no helical unwinding occurs at fields lower than the chevron to bookshelf threshold. Resonant features associated with the four-layer ferrielectric liquid crystal phase were observed in a device geometry. Monitoring the electric field dependence of these ferrielectric resonant peaks showed that the chevron to bookshelf transition occurs at a lower applied field than the ferrielectric to ferroelectric switching transition.
The LED and fiber based calibration system for the photomultiplier array of SNO+ (2015)

A new external LED/fiber light injection calibration system was designed for the calibration and monitoring of the photomultiplier array of the SNO+ experiment at SNOLAB. The goal of the calibration system is to allow an accurate and regular measurement of the photomultiplier array's performance, while minimizing the risk of radioactivity ingress. The choice in SNO+ was to use a set of optical fiber cables to convey into the detector the light pulses produced by external LEDs. The quality control was carried out using a modified test bench that was used in QC of optical fibers for TileCal/ATLAS. The optical fibers were characterized for transmission, timing and angular dispersions. This article describes the setups used for the characterization and quality control of the system based on LEDs and optical fibers and their results.
Supplementary fig 1 from A Modified Nucleoside 6-Thio-2′-Deoxyguanosine Exhibits Antitumor Activity in Gliomas (2023)

&lt;p&gt;Supplementary Figure 1. (A-B) Successful labeling of CellTrace Violet for M059K and H4 cells. M059K and H4 cells were labeled with a cell membrane dye CellTrace Violet and verified the success of labeling by FACS. (C-D) Cell viability of M059K and H4 following THIO treatment. M059K and H4 cells were treated with control medium or THIO at 5µM and then determined the cell viability percentage via detecting PSVue643 and PI by FACS. (E-F) Western blot results of time-course and dose-titration experiments in M059K and H4. For the time-course experiment, cells were treated with THIO at 5µM and proteins were tested at 0, 24, 48 and 72 hours. For dose-titration experiments, cells were treated with serious doses of THIO for 72 hours. TMZ functions as control. Actin serves as a loading control.&lt;/p&gt;
CD30/CD16A Tandab AFM13-Induced Target Cell Lysis By NK-Cells Is Enhanced By CD137 Co-Stimulation and Blocking PD-1 (2015)

Abstract Introduction: AFM13 is a CD30/CD16A bispecific tetravalent TandAb antibody that recruits and activates NK-cells by specific binding to CD16A for targeted lysis of CD30+ tumor cells. Given promising clinical activity and safety profile of AFM13 and proof-of-mechanism demonstrating dependence on the immune response, potential synergy of AFM13 and checkpoint modulators was evaluated. Methods: Efficacy of AFM13 alone or in combination with anti-CTLA-4, anti-PD-1, or anti-CD137 antibodies was assessed by in vitro cytotoxicity assays with human PBMCs or enriched NK-cells and CD30+ target cells as well as patient-derived xenograft in vivo models with autologous PBMC. To evaluate NK-cell-mediated lysis of CD30+ lymphoma cell lines, 4 hour cytotoxicity assays were performed with PBMCs or enriched NK-cells as effector cells in the presence of suboptimal concentrations of AFM13 alone, and in combination with anti-CTLA-4, anti-PD-1, or anti-CD137 antibodies. For the in vivo model tumor fragments derived from surgical specimens of newly diagnosed patients with CD30+ Hodgkin Lymphoma were xenografted (PDX) in immuno-deficient mice. After 28 days mice were reconstituted with autologous patient-derived PBMC and treated with AFM13 alone and in combination with anti-CTLA-4, anti-PD-1, or anti-CD137 antibodies weekly for a total of three weeks. Tumor size, tumor-infiltrating human lymphocytes and intra-tumoral cytokines were evaluated on day 58. Results: AFM13 as a single agent at suboptimal concentrations induced effector-to-target cell-dependent lysis of CD30+ lymphoma cells up to 40% using enriched NK-cells as effector cells in a 4 hour in vitro assay. Immune-modulating antibodies alone mediated substantially lower lysis (&lt;25%). However, the addition of anti-PD-1 or anti-CD137 to AFM13 strongly enhanced specific lysis up to 70%, whereas the addition of anti-CTLA-4 to AFM13 showed no beneficial effect. The most impressive increase of efficacy was observed when AFM13 was applied together with a combination of anti-PD-1 and anti-CD137. In vivo, reduction of tumor growth was observed when AFM13 and anti-PD-1 were used as single agents or when AFM13 was combined with anti-CD137. Synergy was most impressive in these PDX models for the combination of AFM13 and anti-PD-1 which led to a very strong reduction of tumor size. Of note, reduction of tumor growth was strongly correlated with infiltrating NK- and T-cells and intra-tumoral cytokines. Conclusions: The combination trials performed with companion intra-tumoral assessment of lymphocytes and cytokines may enhance the efficacy of AFM13 in patients. This may be explained by a potential cross-talk between NK-cells and T-cell which was enhanced when AFM13 was used in combination with checkpoint modulators. Disclosures No relevant conflicts of interest to declare.
Reversible C−C Bond Cleavage and Interconversion of the Resulting Hydrocarbyl Ligands on Butterfly Frameworks Derived from Acetylide Complexes Cp*WOs3(μ4-CCR)(CO)11(R = Ph,nBu, CH2OMe, CH2OPh) (1997)

Combination of mononuclear complexes Cp*W(CO)3(CCR) (Cp* = C5Me5; R = Ph, nBu, CH2OMe, CH2OPh) with the triosmium cluster Os3(CO)10(NCMe)2 in toluene affords two isomeric acetylide cluster compounds a and b, which possess the formula Cp*WOs3(CCR)(CO)11 (R = Ph (1), nBu (2), CH2OMe (3), CH2OPh (4)). Isomers a and b undergo reversible interconversion by relocating the Cp*W(CO)2 fragment between the hinge and wingtip positions upon heating in solution. Their reactivities vs the substituents on the acetylide ligand are also investigated and compared. Thus, thermolysis of 1a furnishes the carbido−alkylidyne cluster Cp*WOs3(μ4-C)(μ-CPh)(CO)10 (5) through reversible scission of the C−C bond induced by elimination of CO. By contrast, heating of 2 or 3 gives an isomeric mixture of the carbido−vinylidene clusters Cp*WOs3(μ4-C)(μ-H)(μ-CCHR‘)(CO)9 (R‘ = nPr (6), OMe (7)) through a subsequent C−H activation. The CH2OPh isomers 4 readily eliminate two CO ligands to give two isomeric carbido−benzofuryl clusters Cp*WOs3(μ4-C)(μ-H)2(μ-C8H6O)(CO)9 (9 and 10), in which the furyl fragments are produced through subsequent orthometalation involving the phenyl group, C−C bond formation, and H migration. Hydrogenation of 3 produces the dihydrido−acetylide cluster Cp*WOs3(μ-H)2(CCCH2OMe)(CO)10 (11) and the carbido−alkylidyne cluster Cp*WOs3(μ4-C)(μ-H)2(μ-CCH2OMe)(CO)9 (13) subsequently. The acetylide cluster 11 converts to the tetrahedral alkylidyne complex Cp*WOs3(μ3-CCH2CH2OMe)(CO)11 (12) via addition of a CO ligand, whereas the alkylidyne cluster 13 isomerizes upon further heating in solution, giving the alkenyl cluster Cp*WOs3(μ4-C)(μ-H)2(μ-CHCHOMe)(CO)9 (14) via a 1,2-H shift. Spectroscopic data, X-ray structural analyses, and the possible mechanism leading to the interconversions are presented.
Simultaneous sensor localization and target tracking in mine tunnels (2013)

Mine tunnels are extensive labyrinths with irregularly-shaped walls, in which a hundreds of employees are working on extraction of valuable ores and minerals. Since the working conditions are extremely hazardous, a (wireless) sensor network (WSN) is deployed to increase the safety in tunnels. One of the most important applications of WSN is to track the personnel, mobile equipment and vehicles. However, the state-of-the-art algorithms assume that the positions of the sensors are perfectly known, which is not necessarily true due to the imprecise placement and/or possible sensor drops. Therefore, we propose an automatic approach for simultaneous refinement ofsensors’ positions (localization) and target tracking. We use a measurement model from a real mine, and apply a discrete variant of real-time belief propagation, which can efficiently solve this high-dimensional problem, and handle all non-Gaussian uncertainties typical for mining environments. Comparing with standard Bayesian target tracking and localization algorithms, both the sensors’ and the target’s estimates are improved.
Dynamically Composing Domain-Data Selection with Clean-Data Selection by "Co-Curricular Learning" for Neural Machine Translation (2019)

Noise and domain are important aspects of data quality for neural machine translation. Existing research focus separately on domain-data selection, clean-data selection, or their static combination, leaving the dynamic interaction across them not explicitly examined. This paper introduces a "co-curricular learning" method to compose dynamic domain-data selection with dynamic clean-data selection, for transfer learning across both capabilities. We apply an EM-style optimization procedure to further refine the "co-curriculum". Experiment results and analysis with two domains demonstrate the effectiveness of the method and the properties of data scheduled by the co-curriculum.
Hematopoietic Growth Factors Reduce Side Effects of Cancer Treatment: Results of Two Comprehensive Meta-Analyses. (2004)

Abstract Background: Hematopoietic growth factors such as G-CSF/GM-CSF and erythropoietin (EPO) are used in clinical practice to reduce side effects of cancer treatment but also to improve tumour response and overall survival. We conducted two meta-analyses on the effects of hematopoietic growth factors in the treatment of malignant diseases a) to determine the effectiveness of G-CSF and GM-CSF to i) prevent infection, ii) improve tumor response and overall survival in lymphoma patients undergoing standard chemotherapy and b) to assess the effectiveness of EPO to i) prevent anemia, ii) improve tumor response and overall survival in cancer patients. Methods: The Cochrane Library, Medline, Embase and smaller databases were searched. Randomized controlled trials comparing G-CSF/GM-CSF or EPO versus placebo/no treatment were included in the reviews. Both study arms had to receive identical antineoplastic treatment and identical additional supportive care. We included full-text and abstract publications as well as unpublished data. Data were meta-analysed using fixed and random effects models. Results: Twenty one trials with 2,794 adults were included in the EPO meta-analysis. Use of EPO significantly reduced the relative risk of red blood cell transfusions (RR 0.67; 95% CI 0.62 to 0.73). For participants with baseline hemoglobin below 10 g/dL hematological response was observed more often in participants receiving EPO (RR 3.60; 95% CI 3.07 to 4.23). There was inconclusive evidence whether EPO improves tumour response (random effects: RR 1.21; 95% CI 0.92–1.59) and overall survival (unadjusted data: HR 0.84; 95% CI 0.69 to 1.02). 12 trials with 1,823 randomized patients were included in the G-CSF/GM-CSF meta-analysis. Compared with no prophylaxis, G-CSF/GM-CSF significantly reduced the relative risk for infections (RR 0.74; 95% CI 0.64–0.85). There was no evidence for G-CSF/GM-CSF to decrease infection related mortality (RR 1.37; 95% CI 0.66–2.32), to improve complete response (RR 1.02; 95% CI 0.94–1.11) or overall survival (HR 1.00; 95% CI 0.86–1.16). Conclusions: There is consistent evidence that the administration of EPO and G-CSF/GM-CSF reduces the side effects of antineoplastic therapy, such as risk for blood transfusions (EPO) and infections (G-CSF/GM-CSF). However, there is inconclusive evidence whether the administration of hematopoietic growth factors in these clinical settings also improve tumor response and overall survival.