gebhart/patrepeval huggingface dataset:
Conversion of bovine growth hormone cysteine residues to serine affects secretion by cultured cells and growth rates in transgenic mice. (1992)

GHs have been found to possess two disulfide bonds. We set out to determine the importance of bovine (b) GH's disulfide bonds relative to the ability of the hormone to be secreted by cultured cells in vitro and to promote growth in transgenic mice. We have generated six mutated bGH genes that encode serine (Ser) substitutions for cysteines (Cys). These mutated genes were used to generate bGH analogs in which either one or both disulfide bonds are destroyed. When the small loop of bGH was destroyed (Cys181-Ser or Cys189-Ser), the bGH analogs were found to be secreted by mouse L-cells at levels comparable to those of wild-type bGH. However, secretion was drastically reduced when the large loop was abolished (Cys53-Ser or Cys164-Ser). An immunofluorescence study of these bGH analogs revealed two distinct patterns of subcellular localization. Bovine GH analogs with mutations in the small loop demonstrated a perinuclear distribution similar to that of wild-type bGH, but analogs containing a disrupted large loop revealed a uniform cytoplasmic distribution pattern. When these mutated bGH genes were individually introduced into transgenic mice, only those animals that expressed bGH analogs with the large loop intact demonstrated a growth-enhanced phenotype. Transgenic mice that expressed bGH analogs lacking the large loop showed growth rates similar to those of nontransgenic mice. These results suggest that the integrity of the large loop, but not that of the small loop, is essential for the growth-enhancing activity of bGH in transgenic mice.
Developing Ovarian Cancer Sensors Using Molecular Perceptron (2021)

Ovarian cancer patient prognosis and quality of life are significantly affected by the failure to accurately diagnose disease at early stage. Currently, less than 15% of ovarian cancer patients are diagnosed at stage 1, when 5-year survival rates are near 93%. Early detection may save more than 80% of women diagnosed with ovarian cancer. Our goal is to develop clinical long-term implantable carbon nanotube-based sensors for ovarian cancer biomarkers to transiently detect and monitor their levels in patients. The proposed sensors will harness the unique optical properties and sensitivity of single-walled carbon nanotubes. To improve sensitivity and specificity of such sensors, previously developed to detect ovarian cancer biomarkers in vivo, we used molecular perceptron—data analytics-based methods to detect specific spectroscopic fingerprints from the binding of protein biomarkers to the nanotube surface.
Plenary talks: A vibrotactile sensory substitution device for the deaf and profoundly hearing impaired (2014)

There are 2 million functionally deaf individuals in the United States and an estimated 53 million worldwide. The cochlear implant (CI) is an effective solution for regaining hearing capabilities for certain populations within this group, but not for all. First, CIs are expensive, ranging from $40,000 to $90,000. Second, CIs require invasive surgery. Third, there is low efficacy of late CI implantation in early-onset deaf adults. Given these considerations, millions of deaf individuals would benefit from a hearing replacement that has low cost, does not involve an invasive procedure, and may have a higher efficacy for early-onset deaf adults. To this end, we are developing a low-cost, non-invasive, plasticity-based solution to deliver auditory information to the brain. Specifically, we are developing a “vibratory vest” by which auditory information is captured, digitally processed, and delivered to the skin of the torso using small vibratory motors. The term for such a technique is sensory substitution, and has previously proven successful in allowing those who are blind to have visual experience through the tongue or skin. In this talk, we will present our fully functional, real-time, Bluetooth-operated prototype, and demonstrate our results from several speech perception experiments. Funding for this research is supported by a training fellowship from the Keck Center of Interdisciplinary Bioscience Training of the Gulf Coast Consortia (NIBIB Grant No. 5T32EB006350-05) and the Renz Foundation.
Simultaneous 3D acquisition of1H MRF and23Na MRI (2021)

With the 3D simultaneous implementation, we were able to acquire sodium and proton density weighted images in addition to proton T<sub>1</sub> , T<sub>2</sub> , and <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:msubsup><mml:mi>B</mml:mi><mml:mn>1</mml:mn><mml:mo>+</mml:mo></mml:msubsup></mml:math> from <sup>1</sup> H MRF that covers the whole brain volume within 21 min.
Toward automated assignment of nuclear magnetic resonance spectra: pattern recognition in two-dimensional correlation spectra (1985)

Computer anal. of the multiplet structure of cross peaks in phase-sensitive 2-dimensional NMR correlation spectra allows one to trace out networks of coupled spins, to measure the magnitudes and signs of the scalar coupling consts., and to det. the no. of magnetically equiv. spins at each site. Applications to mixts. of smallmols. show that pattern recognition is feasible even if the signal-to-noise ratio is low, if the multiplets are barely resolved, or if the patterns are partly disguised because of accidentally overlapping cross peaks. [on SciFinder (R)]
Isolation and Characterization of a Split B-type DNA Polymerase from the Archaeon Methanobacterium thermoautotrophicumΔH (1999)

We describe here the isolation and characterization of a B-type DNA polymerase (PolB) from the archaeon Methanobacterium thermoautotrophicum DeltaH. Uniquely, the catalytic domains of M. thermoautotrophicum PolB are encoded from two different genes, a feature that has not been observed as yet in other polymerases. The two genes were cloned, and the proteins were overexpressed in Escherichia coli and purified individually and as a complex. We demonstrate that both polypeptides are needed to form the active polymerase. Similar to other polymerases constituting the B-type family, PolB possesses both polymerase and 3'-5' exonuclease activities. We found that a homolog of replication protein A from M. thermoautotrophicum inhibits the PolB activity. The inhibition of DNA synthesis by replication protein A from M. thermoautotrophicum can be relieved by the addition of M. thermoautotrophicum homologs of replication factor C and proliferating cell nuclear antigen. The possible roles of PolB in M. thermoautotrophicum replication are discussed.
Long-term Survivorship and Failure Modes of Unicompartmental Knee Arthroplasty (2012)

With this cemented, fixed-bearing design, the failure rates were low, there were no cases of failure secondary to wear or loosening, and the survivorship was similar to that reported for total knee arthroplasty.
A survey of B-tree logging and recovery techniques (2012)

B-trees have been ubiquitous in database management systems for several decades, and they serve in many other storage systems as well. Their basic structure and their basic operations are well understood including search, insertion, and deletion. However, implementation of transactional guarantees such as all-or-nothing failure atomicity and durability in spite of media and system failures seems to be difficult. High-performance techniques such as pseudo-deleted records, allocation-only logging, and transaction processing during crash recovery are widely used in commercial B-tree implementations but not widely understood. This survey collects many of these techniques as a reference for students, researchers, system architects, and software developers. Central in this discussion are physical data independence, separation of logical database contents and physical representation, and the concepts of user transactions and system transactions. Many of the techniques discussed are applicable beyond B-trees.
Vertical n-Channel Poly-Si Thin-Film Transistors With Symmetric S/D Fabricated by Ni-Silicide-Induced Lateral-Crystallization Technology (2009)

We have successfully developed and fabricated the vertical n-channel polycrystalline silicon thin-film transistors with symmetric S/D fabricated by Ni-silicide-induced lateral-crystallization technology (NSILC-VTFTs). The NSILC-VTFTs are S/D symmetric devices and equivalent to dual-gate devices. The dual-gate structure of NSILC-VTFTs can moderate the lateral electrical field in the drain depletion region, significantly reducing the leakage current. In NSILC-VTFTs, the Ni accumulation and grain boundaries induced from S/D sides can be centralized in the n <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">+</sup> floating region. The effects of Ni accumulation in symmetric VTFTs crystallized by NSILC and metal-induced lateral crystallization are studied. In addition, a two-step lateral crystallization has been introduced to improve the crystal integrity through secondary crystallization. The NSILC-VTFTs crystallized by two-step lateral crystallization show a steep subthreshold swing of 180 mV/dec and field effect mobility mu = 553 cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> /Vmiddots without NH <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> plasma treatment.
Impact of Barrier Height on the Interwell Carrier Transport in InGaN/(In)GaN Multiple Quantum Wells (2019)

Interwell carrier transport, important for efficient LED and laser diode operation, was studied in InGaN multiple quantum wells by time-resolved photoluminescence. A strong increase in transport efficiency was achieved in structures in which GaN barriers were replaced with that of InGaN.
Supplementary Figures and Tables from PCTAIRE1 Phosphorylates p27 and Regulates Mitosis in Cancer Cells (2023)

&lt;p&gt;Supplementary Figures and Tables. Supplementary Fig. S1. Knockdown of PCTAIRE1 in PPC1 cells. Supplementary Fig. S2. Knockdown of PCTAIRE1 inhibits growth of Du145, MDA-MB-468 and HeLa cells. Supplementary Fig. S3. PCTAIRE1 regulates centrosome dynamics. Supplementary Fig. S4. PCTAIRE1 phosphorylates p27 at Ser10. Supplementary Fig. S5. PCTAIRE1 knockdown leads to accumulation of p27. Supplementary Fig. S6. Apoptosis induced by PCTAIRE1 knockdown, and subcellular localization of Eg5 in PCTAIRE1 knockdown cancer cells. Supplementary Fig. S7. Characterization of rabbit anti-PCTAIRE1 antibody and correlation of PCTAIRE1 and p27 expression. Supplementary Fig. S8. PCTAIRE1 expression is upregulated in cancers, and high PCTAIRE1 levels correlate with poor patient prognosis. Supplementary Table S1. Specific interaction of PCTAIRE1 with p27.&lt;/p&gt;
Emission property of Ce^3+-doped Li_2O-B_2O_3-SiO_2 glasses (2015)

Optical properties of Ce3+-doped Li2O-B2O3-SiO2 glasses prepared in inert atmosphere have been examined. Clear splitting of 4f-5d excitation bands is observed in these glasses at room temperature. The emission decay constant depends on the excitation and emission energy, indicating the site distribution of Ce3+ in the random glass matrix. The absorption energies of Ce3+ are independent of the optical basicity, suggesting that the emission is affected by the local basicity of random network. The present findings provide the basic concepts for designing Ce-doped oxide glass for luminescent applications.
Field-Measured Performance Evaluation of a Digital Daylighting System (2010)

In 2005, the largest field study to-date concluded that automated dimming controls in side-lighted spaces consistently underperformed relative to expectation, and were still to be considered an ‘emerging’ technology. Motivated by a desire to accelerate the adoption and acceptance of such controls in, this paper details a field-measured assessment of a digital daylighting system recently introduced to the US market. Two intentionally challenging offices were retrofit with controls, and automated data acquisition hardware, and system performance was evaluated across several parameters throughout a twelve-month period.The field data and user interviews indicate that the system was relatively straightforward to commission compared to other dimming controls, and overall was well received by the occupants. Energy savings in the private and open-plan installations were between 17 percent and 20 percent. In the private office, the pre-existence of occupancy sensing, and automatic-ON in the retrofit system precluded even deeper energy savings, whereas false triggering of the occupancy sensor limited the achieved savings in the open plan space. The controller was largely able to maintain target illuminance on the worksurface, although increased daylight availability led to increased variability and a 100-lx increase between noon and 2PM. Key recommendations to increase the acceptance of similar systems include the provision of shielding options for occupancy sensors, manual-ON options for private offices, remote-access commissioning tools, and improved clarity of written materials describing system function, operation, and installation.
Measuring Self-Assembly in Solution:  Incorporation and Dynamics of a “Tailor-Made Impurity” in Precrystalline Glucose Aggregates (1996)

We have studied the onset of crystallization from solution using a fluorescent probe molecule that incorporates selectively into precrystalline glucose aggregates that form in supersaturated aqueous glucose solutions. We achieve incorporation of the fluorophore into the aggregates by virtue of the fluorophore pendant glycosyl moiety and compare the rotational diffusion data for this molecule to that for the nonglycosylated, native probe molecule. This experimental approach, in conjunction with semiempirical calculations to understand the electronic response of the fluorescent probe, provides insight into the formation and size of precrystalline glucose aggregates. Our data indicate that the aggregates effectively isolate the fluorophore from the solution over a range of glucose concentrations spanning the saturation point and that the lifetime of these aggregates is on the order of a nanosecond for aggregates that include the glycosylated probe molecule. The subtle but important differences between these results and those we reported previously for carminic acid in aqueous glucose solutions point to the significant role of labile protons in mediating the formation and dynamics of precrystalline glucose aggregates.
Managing Post-fault Oscillation Phenomenon in Compensated MV-networks (2016)

Post-fault oscillations have recently been identified as being one of the main causes of maloperations of earth-fault protection in compensated MV-networks [1]. Until now, the theory regarding post-fault oscillation condition has been missing from `classical' earth-fault protection analysis, e.g. [2], where only healthy-state, inside and outside fault conditions have been considered. This paper adds this `missing piece of the puzzle' and presents the necessary theory and hand-calculation equations to analyse the post-fault oscillation condition from earth-fault protection perspective in the level required by a protection engineer.
New Life for an Old Antibiotic (2015)

Restoring the antibacterial properties of existing antibiotics is of great concern. Herein, we present, for the first time, the formation and deposition of stable antibiotic nanoparticles (NPs) on graphene oxide (GO) sheets by a facile one-step sonochemical technique. Sonochemically synthesized graphene oxide/tetracycline (GO/TET) composite shows enhanced activity against both sensitive and resistant Staphylococcus aureus (S. aureus). The size and deposition of tetracycline (TET) nanoparticles on GO can be controlled by varying the sonication time. The synthesized NPs ranged from 21 to 180 nm. Moreover, ultrasonic irradiation does not cause any structural and chemical changes to the TET molecule as confirmed by Fourier transform infrared spectroscopy (FTIR). The virtue of π-π stacking between GO and TET additionally facilitate the coating of TET NPs upon GO. A time dependent release kinetics of TET NPs from the GO surface is also monitored providing important insights regarding the mechanism of antibacterial activity of GO/TET composites. Our results show that the GO/TET composite is bactericidal in nature, resulting in similar values of minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). This composite is found to be active against TET resistant S. aureus at a concentration four times lower than the pristine TET. The sensitive S. aureus follows the same trend showing six times lower MIC values compared to pristine TET. GO shows no activity against both sensitive and resistant S. aureus even at a concentration as high as 1 mg/mL but influences the biocidal activity of the GO/TET composite. We propose that the unique structure and composition manifested by GO/TET composites may be further utilized for different formulations of antibiotics with GO. The sonochemical method used in this work can be precisely tailored for the stable deposition of a variety of antibiotics on the GO surface to reduce health risks and increase the spectrum of applications.
Alternative splicing in the pore-forming region of shaker potassium channels. (1997)

We have cloned cDNAs for the shaker potassium channel gene from the spiny lobster Panulirus interruptus. As previously found in Drosophila, there is alternative splicing at the 5' and 3' ends of the coding region. However, in Panulirus shaker, alternative splicing also occurs within the pore-forming region of the protein. Three different splice variants were found within the P region, two of which bestow unique electrophysiological characteristics to channel function. Pore I and pore II variants differ in voltage dependence for activation, kinetics of inactivation, current rectification, and drug resistance. The pore 0 variant lacks a P region exon and does not produce a functional channel. This is the first example of alternative splicing within the pore-forming region of a voltage-dependent ion channel. We used a recently identified potassium channel blocker, kappa-conotoxin PVIIA, to study the physiological role of the two pore forms. The toxin selectively blocked one pore form, whereas the other form, heteromers between the two pore forms, and Panulirus shal were not blocked. When it was tested in the Panulirus stomatogastric ganglion, the toxin produced no effects on transient K+ currents or synaptic transmission between neurons.
On MIMO for UTRA LTE (2008)

The long term evolution of UMTS (Universal Mobile Telecommunications System), UTRA LTE, is based on OFDM (orthogonal frequency division multiplexing) combined with MIMO (multiple-input multiple-output) techniques. In particular, the Alamouti and the V-BLAST (Vertical Bell Labs Layered Space Time) schemes are seen as interesting MIMO concepts. In this communication, the authors will compare these two MIMO schemes w.r.t. the achievable performance in the UTRA LTE downlink using up to two transmit and two receive antennas.
The virome of the mosquito vector Culex pipiens (2017)

There is a dramatic lack of data on the virome of arthropods transmitting pathogenic viruses for humans and livestock. For example, we do not yet know whether there is a group of virus species that are predominant and conserved in the virome of many important vectors no matter the environment. These putative core virome could influence vector competence and other aspects of vector biology, as observed in the bacteriome of mosquito vectors. The study of the virome can thus unveil unknown aspects of the vector lifecycle, a knowledge that could empower vector control strategies. We have analysed the virome of Culex pipiens, a mosquito vector of important arboviruses like Rift Valley fever virus or West Nile fever virus, coupling a metagenomics approach and a large sampling campaign around the Mediterranean Sea. Our results show for the first time conserved patterns in diversity and structure within the virome of an important mosquito vector as well as specificities probably linked to different environmental conditions. (Texte intégral)
ChemInform Abstract: Molecular Docking of Cathepsin L Inhibitors in the Binding Site of Papain. (2008)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Automatic 4D Flow MRI Segmentation Using the Standardized Difference of Means Velocity (2023)

We present a method to automatically segment 4D flow magnetic resonance imaging (MRI) by identifying net flow effects using the standardized difference of means (SDM) velocity. The SDM velocity quantifies the ratio between the net flow and observed flow pulsatility in each voxel. Vessel segmentation is performed using an F-test, identifying voxels with significantly higher SDM velocity values than background voxels. We compare the SDM segmentation algorithm against pseudo-complex difference (PCD) intensity segmentation of 4D flow measurements in in vitro cerebral aneurysm models and 10 in vitro Circle of Willis (CoW) datasets. We also compared the SDM algorithm to convolutional neural network (CNN) segmentation in 5 thoracic vasculature datasets. The in vitro flow phantom geometry is known, while the ground truth geometries for the CoW and thoracic aortas are derived from high-resolution time-of-flight (TOF) magnetic resonance angiography and manual segmentation, respectively. The SDM algorithm demonstrates greater robustness than PCD and CNN approaches and can be applied to 4D flow data from other vascular territories. The SDM to PCD comparison demonstrated an approximate 48% increase in sensitivity in vitro and 70% increase in the CoW, respectively; the SDM and CNN sensitivities were similar. The vessel surface derived from the SDM method was 46% closer to the in vitro surfaces and 72% closer to the in vitro TOF surfaces than the PCD approach. The SDM and CNN approaches both accurately identify vessel surfaces. The SDM algorithm is a repeatable segmentation method, enabling reliable computation of hemodynamic metrics associated with cardiovascular disease.
The Value of Establishing the Quantitative Accuracy of PET/CT Imaging (2015)

A total of 1,665,540 new cancer cases and 585,720 deaths from cancer are projected to have occurred for the United States in 2014, and 1 in 4 deaths in the United States will have been from cancer ([1][1]). The reasons for the increasing burden of cancer to society and the meager progress are
Fiber-optic Raman spectroscopy for cure monitoring of advanced polymer composites (1991)

The curing reaction of an epoxy matrix used for wet filament-wound composites was monitored using Raman spectroscopy measured over fiber optics. The resin system consists of the diglycidyl ether of bisphenol-A in combination with a polyethertriamine hardener in a 1:1 stoichiometric ratio. The extent of chemical reaction of the epoxy as a function of time was measurable through changes in peak heights of several vibrational modes. A Raman peak associated with a phenyl ring vibration in epoxide component was used as in internal reference to correct for density changes and instrumental variations. Temperature measurements were made over the same fiber optics used to obtain the cure chemistry data by measuring the intensity of anti-Stokes Raman scattering from the epoxy. Both single-fiber and dual-fiber probes were evaluated.