1973 THE EFFECT OF CHITOSAN ON UROTHELIAL PERMEABILITY, URETERAL INTRALUMINAL DRUG ADMINISTRATION AND PERISTALSIS (2010)

You have accessJournal of UrologyStone Disease: Basic Research1 Apr 20101973 THE EFFECT OF CHITOSAN ON UROTHELIAL PERMEABILITY, URETERAL INTRALUMINAL DRUG ADMINISTRATION AND PERISTALSIS Donald Pick, Stanislav Shelkovnikov, Noah Canvasser, Narges Alipanah, Phillip Mucksavage, Jason Lee, Michael Louie, Elspeth McDougall, and Ralph Clayman Donald PickDonald Pick More articles by this author , Stanislav ShelkovnikovStanislav Shelkovnikov More articles by this author , Noah CanvasserNoah Canvasser More articles by this author , Narges AlipanahNarges Alipanah More articles by this author , Phillip MucksavagePhillip Mucksavage More articles by this author , Jason LeeJason Lee More articles by this author , Michael LouieMichael Louie More articles by this author , Elspeth McDougallElspeth McDougall More articles by this author , and Ralph ClaymanRalph Clayman More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.1983AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Chitosan is the deacetylation product of chitin and has been demonstrated to disrupt urothelial barrier of the bladder. In an ex vivo model, intraluminal nifedipine has been shown to relax the ureter. Relaxing the ureter prior to endourology procedures could ease instrument access. Continuing our work with medications to increase ureteral permeability, we study the effect of 1 μM nifedipine on peristalsis before and after pretreating the ureter with chitosan. METHODS Using freshly harvested porcine ureters, 4 cm intact tubular segments were placed in a novel organ bath with gassed Krebs solution. Both ends of the ureter were cannulated. A pressure transducer was placed in-series at the inflow end of each ureter. Outflow drained by gravity. Intraluminal flow (1 ml/min) was maintained by a gravity fed constant pressure reservoir. To induce peristalsis, phenylephrine (10 μM), was added to the external bath. Nifedipine (1 μM) was then added to the intraluminal reservoir. The time required to stop peristalsis was measured. Chitosan (0.5% w/v, 30 minutes) was then used to treat the urothelium and the study repeated. RESULTS Intraluminal nifedipine (1 μM) had no effect on ureteral peristalsis after 60 minutes of exposure in six trials. After pretreatment with chitosan, nifedipine (1 μM) stopped ureteral peristalsis within an average of 12.30 ± 1.93 minutes. Ureteral contractile amplitudes were similar (p=0.53) before and after chitosan administration. Histological analysis of the ureter before and after pretreatment with chitosan showed no urothelial disruption. CONCLUSIONS By pretreating the intraluminal surface of the ureter with chitosan nifedipine reversibly blocks peristalsis at low concentrations. Chitosan changes ureteral urothelial permeability, but has no long-term effect on ureteral contraction. Orange, CA© 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183 Issue 4S April 2010 Page: e766 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.Metrics Author Information Donald Pick More articles by this author Stanislav Shelkovnikov More articles by this author Noah Canvasser More articles by this author Narges Alipanah More articles by this author Phillip Mucksavage More articles by this author Jason Lee More articles by this author Michael Louie More articles by this author Elspeth McDougall More articles by this author Ralph Clayman More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...