gebhart/patrepeval huggingface dataset:
Essential and Instructive Roles of GATA Factors in Eosinophil Development (2002)

GATA transcription factors are major regulators of hematopoietic and immune system. Among GATA factors, GATA-1, GATA-2, and GATA-3 play crucial roles in the development of erythroid cells, hematopoietic stem, and progenitor cells, and T helper type 2 (Th2) cells, respectively. A high level of GATA-1 and GATA-2 expression has been observed in eosinophils, but their roles in eosinophil development remain uncertain both in vitro and in vivo. Here we show that enforced expression of GATA-1 in human primary myeloid progenitor cells completely switches myeloid cell fate into eosinophils. Expression of GATA-1 exclusively promotes development and terminal maturation of eosinophils. Functional domain analyses revealed that the COOH-terminal finger is essential for this capacity while the other domains are dispensable. Importantly, GATA-1-deficient mice failed to develop eosinophil progenitors in the fetal liver. On the other hand, GATA-2 also showed instructive capacity comparable to GATA-1 in vitro and efficiently compensated for GATA-1 deficiency in terms of eosinophil development in vivo, indicating that proper accumulation of GATA factors is critical for eosinophil development. Taken together, our findings establish essential and instructive roles of GATA factors in eosinophil development. GATA-1 and GATA-2 could be novel molecular targets for therapeutic approaches to allergic inflammation.
ARSENAL: Automatic Requirements Specification Extraction from Natural\n Language (2014)

Requirements are informal and semi-formal descriptions of the expected\nbehavior of a complex system from the viewpoints of its stakeholders\n(customers, users, operators, designers, and engineers). However, for the\npurpose of design, testing, and verification for critical systems, we can\ntransform requirements into formal models that can be analyzed automatically.\nARSENAL is a framework and methodology for systematically transforming natural\nlanguage (NL) requirements into analyzable formal models and logic\nspecifications. These models can be analyzed for consistency and\nimplementability. The ARSENAL methodology is specialized to individual domains,\nbut the approach is general enough to be adapted to new domains.\n
Design Solutions for Increased Thresholds of Non-Linear Processes in Silica Fiber (2019)

Fiber design strategies for increased threshold of SBS and TMI are presented. We report a high efficiency erbium nanoparticle doped fiber incorporating acoustic guiding layers with a significantly reduced SBS gain coefficient.
Obtusaquinone: A Cysteine-Modifying Compound That Targets Keap1 for Degradation (2020)

We have previously identified the natural product obtusaquinone (OBT) as a potent antineoplastic agent with promising in vivo activity in glioblastoma and breast cancer through the activation of oxidative stress; however, the molecular properties of this compound remained elusive. We used a multidisciplinary approach comprising medicinal chemistry, quantitative mass spectrometry-based proteomics, functional studies in cancer cells, and pharmacokinetic analysis, as well as mouse xenograft models to develop and validate novel OBT analogs and characterize the molecular mechanism of action of OBT. We show here that OBT binds to cysteine residues with a particular affinity to cysteine-rich Keap1, a member of the CUL3 ubiquitin ligase complex. This binding promotes an overall stress response and results in ubiquitination and proteasomal degradation of Keap1 and downstream activation of the Nrf2 pathway. Using positron emission tomography (PET) imaging with the PET-tracer 2-[18F]fluoro-2-deoxy-d-glucose (FDG), we confirm that OBT is able to penetrate the brain and functionally target brain tumors. Finally, we show that an OBT analog with improved pharmacological properties, including enhanced potency, stability, and solubility, retains the antineoplastic properties in a xenograft mouse model.
The Delivery of Oligonucleotides Using pH Sensitive Liposomes (2017)

This chapter reviews on the usefulness of pH-sensitive liposomes for the delivery of oligonucleotides without residence time within lysosomes. The effect of divalent cations may be an important parameter to take into account in the design of pH-sensitive liposomes. In fact, in cells the factors that control drug transfer by those vectors are still poorly understood; cytoplasmic delivery by these pH-sensitive liposomes appears incomplete. About half of the total cell-associated pH-sensitive immunoliposomes delivered their content into the target cell cytoplasm. The use of pH-sensitive liposomes in therapeutics would require that vehicles remain stable in the circulation until binding and subsequent endocytosis by target cells. The goal would be the production of antigen-specific, pH-sensitive liposomes that combine access to target cells in tissues and the efficient delivery of liposome contents in the cytoplasm. The requirement for acid sensitivity in the design was demonstrated when pH-insensitive immunoliposomes did not mediate efficient transfection.
MiR ‐182‐3p targets TRF2 and impairs tumor growth of triple‐negative breast cancer (2022)

The telomeric repeat-binding factor 2 (TRF2) is a telomere-capping protein that plays a key role in the maintenance of telomere structure and function. It is highly expressed in different cancer types, and it contributes to cancer progression. To date, anti-cancer strategies to target TRF2 remain a challenge. Here, we developed a miRNA-based approach to reduce TRF2 expression. By performing a high-throughput luciferase screening of 54 candidate miRNAs, we identified miR-182-3p as a specific and efficient post-transcriptional regulator of TRF2. Ectopic expression of miR-182-3p drastically reduced TRF2 protein levels in a panel of telomerase- or alternative lengthening of telomeres (ALT)-positive cancer cell lines. Moreover, miR-182-3p induced DNA damage at telomeric and pericentromeric sites, eventually leading to strong apoptosis activation. We also observed that treatment with lipid nanoparticles (LNPs) containing miR-182-3p impaired tumor growth in triple-negative breast cancer (TNBC) models, including patient-derived tumor xenografts (PDTXs), without affecting mouse survival or tissue function. Finally, LNPs-miR-182-3p were able to cross the blood-brain barrier and reduce intracranial tumors representing a possible therapeutic option for metastatic brain lesions.
Two Domains That Control Prefusion Stability and Transport Competence of the Measles Virus Fusion Protein (2006)

Most viral glycoproteins mediating membrane fusion adopt a metastable native conformation and undergo major conformational changes during fusion. We previously described a panel of compounds that specifically prevent fusion induced by measles virus (MV), most likely by interfering with conformational rearrangements of the MV fusion (F) protein. To further elucidate the basis of inhibition and better understand the mechanism of MV glycoprotein-mediated fusion, we generated and characterized resistant MV variants. Spontaneous mutations conferring drug resistance were confirmed in transient assays and in the context of recombinant virions and were in all cases located in the fusion protein. Several mutations emerged independently at F position 462, which is located in the C-terminal heptad repeat (HR-B) domain. In peptide competition assays, all HR-B mutants at residue 462 revealed reduced affinity for binding to the HR-A core complex compared to unmodified HR-B. Combining mutations at residue 462 with mutations in the distal F head region, which we had previously identified as mediating drug resistance, causes intracellular retention of the mutant proteins. The transport competence and activity of the mutants can be restored, however, by incubation at reduced temperature or in the presence of the inhibitory compounds, indicating that the F escape mutants have a reduced conformational stability and that the inhibitors stabilize a transport-competent conformation of the F trimer. The data support the conclusion that residues located in the head domain of the F trimer and the HR-B region contribute jointly to controlling F conformational stability.
63. EVALUATION OF RESECTABILITY OF HEPATIC MALIGNANCY BY ANGIOGRAPHY, C.T.-ANGIOGRAPHY AND MAGNETIC RESONANCE IMAGING (1987)

Pessar, M. L. M.D.; Zerhouni, E. A. M.D.; Sitzmann, J. M.D.; Fishman, E. M.D.; Kaufman, S. M.D.; Soulen, R. L. M.D. Author Information
Emulating media defect corrosion (2005)

This paper presents studies designed toward understanding media defect corrosion. Focused ion beam technology is used to create well-defined defects exposing alloys of interest. Temperature and humidity stressing is employed to facilitate defect corrosion. Atomic force microscopy is used to characterize the defects before and after environmental stressing. It is found that corrosion growth is largely affected by the exposed alloy corrosion sensitivity. Interaction with overcoat type and lubricant is also discussed.
Performance of DMSA algorithm in hybrid sensor networks (2013)

We report results on dynamic medium switching algorithm (DMSA). This algorithm is used to decide on which interface to use when hybrid nodes are present in a wireless sensor networks. These hybrid nodes have a power line communications (PLC) interface as well as a radio frequency (RF) interface. The algorithm decision on selecting the appropriate interface for communications takes into account long-term stability of the link, packet error rate on the link, it uses an exponential backoff as well as it defers notification to routing layer to avoid fluctuations in routing decisions. The obtained results show that the algorithm improves packet delivery, lowers routing overhead, and improves the overall network lifetime.
ChemInform Abstract: Concise and Efficient Synthesis of 1H‐Pyrazoles (II): Reaction of ( Hydroxy(tosyloxy)iodo)benzene with Ethyl 2,3‐Dioxobutanoate‐2‐ arylhydrazones (I). (1992)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Analytical evaluation of a new point of care system for measuring cardiac Troponin I (2016)

The Minicare cTnI assay is a sensitive and precise, clinical usable test for determination of cTnI concentration that can be used in a near-patient setting as an aid in the diagnosis of acute myocardial infarction.
Current concepts in the diagnosis and pathobiology of intraepithelial neoplasia: A review by organ system (2016)

Answer questions and earn CME/CNE In this report, a team of surgical pathologists has provided a review of intraepithelial neoplasia in a host of (but not all) anatomic sites of interest to colleagues in various medical specialties, namely, uterine cervix, ovary, breast, lung, head and neck, skin, prostate, bladder, pancreas, and esophagus. There is more experience with more readily accessible sites (such as the uterine cervix and skin) than with other anatomic sites, and the lack of uniform terminology, together with divergent biology in various sites, makes it difficult to paint a unifying, relevant portrait. The authors' aim was to provide a framework from which to move forward as we care for patients with such precancerous lesions. CA Cancer J Clin 2016;66:408-436. © 2016 American Cancer Society.
Equivalencing the collector system of a large wind power plant (2006)

As the size and number of wind power plants (also called wind farms) increases, power system planners will need to study their impact on the power system in more detail. As the level of wind power penetration into the grid increases, the transmission system integration requirements becomes more critical. A very large wind power plant may contain hundreds of megawatt-size wind turbines. These turbines are interconnected by an intricate collector system. While the impact of individual turbines on the larger power system network is minimal, collectively, wind turbines can have a significant impact on the power systems during a severe disturbance such as a nearby fault. Since it is not practical to represent all individual wind turbines to conduct simulations, a simplified equivalent representation is required. This paper focuses on our effort to develop an equivalent representation of a wind power plant collector system for power system planning studies. The layout of the wind power plant, the size and type of conductors used, and the method of delivery (overhead or buried cables) all influence the performance of the collector system inside the wind power plant. Our effort to develop an equivalent representation of the collector system for wind power plants is an attempt to simplify power system modeling for future developments or planned expansions of wind power plants. Although we use a specific large wind power plant as a case study, the concept is applicable for any type of wind power plant
Aminoacyl RNA domain of turnip yellow mosaic virus Val-RNA interacting with elongation factor Tu (1984)

Turnip yellow mosaic virus (TYMV) Val-RNA forms a complex with the peptide elongation factor Tu (EF-Tu) in the presence of GTP: the Val-RNA is protected by EF-Tu.GTP from non-enzymatic deacylation and nuclease digestion. The determination of the length of the shortest TYMV Val-RNA fragment that binds EF-Tu.GTP leads us to conclude that the valylated aminoacyl RNA domain equivalent in tRNAs to the continuous helix formed by the acceptor stem and the T arm is sufficient for complex formation. Since the aminoacyl RNA domain is also sufficient for adenylation by the ATP(CTP):tRNA nucleotidyltransferase, an analogy can be drawn between these two tRNA-specific proteins.
Advancing hydrological process understanding from long‐term resistivity monitoring systems (2021)

Abstract Monitoring subsurface flow and transport processes over a wide range of spatiotemporal scales remains one of the greatest challenges in hydrology. Electrical geophysical techniques have been implemented to noninvasively investigate a broad range of subsurface hydrological processes. Recent advances in instrumentation and interpretational tools highlight the emerging opportunities to adopt long‐term resistivity monitoring (LTRM) to improve understanding of flow and transport processes operating over monthly to decadal timescales that are not adequately captured in short‐term monitoring data sets and are temporally aliased in data sets constructed from occasional reoccupation of a study site. The emergence of LTRM as a robust tool in hydrology represents a paradigm shift in geophysical data acquisition and analysis, with resistivity monitoring now evolving into a hydrological decision support technology. We describe the theoretical basis for adopting LTRM for noninvasive monitoring of hydrological state variables over multiple spatial scales and with higher temporal resolution than achieved from periodic reoccupation of a field site. Instrumentation developments facilitating autonomous data acquisition at off the grid field sites are discussed, along with advances in data processing that enhance the hydrological information content inherent in LTRM data sets. Case studies from a diverse range of hydrology subdisciplines highlight the largely untapped potential for LTRM to provide information beyond the reach of established hydrology tools. Future opportunities and challenges relating to the more widespread adoption of LTRM, including addressing inherent uncertainty in resistivity interpretation, upscaling, computational, and modeling needs are critically discussed. This article is categorized under: Science of Water (WCAA)
Dense and long-term monitoring of Earth surface processes with passive RFID -- a review (2021)

Billions of Radio-Frequency Identification (RFID) passive tags are produced yearly to identify goods remotely. New research and business applications are continuously arising, including recently localization and sensing to monitor earth surface processes. Indeed, passive tags can cost 10 to 100 times less than wireless sensors networks and require little maintenance, facilitating years-long monitoring with ten's to thousands of tags. This study reviews the existing and potential applications of RFID in geosciences. The most mature application today is the study of coarse sediment transport in rivers or coastal environments, using tags placed into pebbles. More recently, tag localization was used to monitor landslide displacement, with a centimetric accuracy. Sensing tags were used to detect a displacement threshold on unstable rocks, to monitor the soil moisture or temperature, and to monitor the snowpack temperature and snow water equivalent. RFID sensors, available today, could monitor other parameters, such as the vibration of structures, the tilt of unstable boulders, the strain of a material, or the salinity of water. Key challenges for using RFID monitoring more broadly in geosciences include the use of ground and aerial vehicles to collect data or localize tags, the increase in reading range and duration, the ability to use tags placed under ground, snow, water or vegetation, and the optimization of economical and environmental cost. As a pattern, passive RFID could fill a gap between wireless sensor networks and manual measurements, to collect data efficiently over large areas, during several years, at high spatial density and moderate cost.
Synergistic effects of leucine and resveratrol on insulin sensitivity and fat metabolism in adipocytes and mice (2012)

These data demonstrate that either leucine or its metabolite HMB may be combined with a low concentration of resveratrol to exert synergistic effects on Sirt1-dependent outcomes; this may result in more practical dosing of resveratrol in the management of obesity, insulin-resistance and diabetes.
BELT (2020)

As online educational programs scale, monitoring peer collaboration in platforms like BlueJeans for plagiarism becomes difficult. Recent studies indicate that students are less likely to cheat if presented with direct warning messages prior to engaging in online activities. In this work, we present Bluejeans codE Leak deTection (BELT), a system that monitors online BlueJeans meetings for shared code and sends timely warning messages to meeting participants. To test BELT's robustness as an online proctor, we evaluate its code-text disambiguation, code detection from images of varying quality, and code detection from videos of varying resolution. We conclude this work by pinpointing areas of improvement and briefly discuss possible extensions for future work.
Brasilidine A, a New Cytotoxic Isonitrile-Containing Indole Alkaloid from the Actinomycete Nocardia brasiliensis (1997)

A new cytotoxic indole alkaloid containing an isonitrile group, brasilidine A (1), has been isolated from the actinomycete Nocardia brasiliensis IFM 0089 and the structure elucidated on the basis of its spectroscopic data.
Temporal global expression profiling of DSS colitis and identification of potential targets of ulcerative colitis (2009)

lamina propria and lymphoid aggregates was evaluated in wildtype mice, 7-/mice, and wildtype mice given blocking antibodies to 47 or control antibodies using flow cytometry and immunohistochemistry. To evaluate the effect of 47 blockade in acute injury, the onset and regression of inflammation, lymphoid aggregate formation and regression and the presence of T-lymphocytes were evaluated in the acute injury model of DSS colitis during 47 blockade. To evaluate the effect of 47 blockade on T-cell mediated colitis, the course of colitis and the presence of T-lymphocytes in the diffuse lamina propria and lymphoid aggregates was evaluated in the splenocyte transfer model of colitis. RESULTS: In the unchallenged animals, the intestinal T-lymphocyte population in the diffuse lamina propria was normal in the absence of 47 function. The T-lymphocyte population in the lymphoid aggregates was also normal with the exception that the Foxp-3 T-lymphocyte population was significantly decreased in the absence of 7. DSS-induced colitis peaked at day 3 after the removal of as DSS demonstrated by increased histological score, MPO activity, and began to regress at day 7 after removal of DSS. The number of B-cell containing lymphoid aggregates peaked at 7 days after the removal of DSS and remained elevated at 14 days after the removal of DSS even though clinical inflammation had regressed. Anti-47 blockade or loss of 7 function dramatically decreased the number B-cell containing lymphoid aggregates, but did not show any other effects, including changes in T-lymphocyte trafficking into the aggregates, during DSS-induced colitis. In the T-cell mediated splenocyte transfer model of colitis, the population of T-lymphocytes in the diffuse lamina propria and the lymphoid aggregates were significantly decreased in 7-/mice and the wildtype mice receiving 47 blockade. This reduction corresponded to the decreased inflammation as evidenced by significant lower expression of TNF-, IFN-, IL-6, MPO and IL-17. CONCLUSIONS: The presence of Foxp3 T-lymphocytes in lymphoid aggregates is dependent upon 47, however the trafficking of other T-lymphocytes into the diffuse lamina propria and lymphoid aggregates in the basal state and during acute injury is not dependent upon 47. 47 blockade can block pathogenic T-lymphocyte trafficking to the intestine in a T-cell mediated model of colitis and accordingly is effective at ameliorating inflammation in this model. These observations suggest that 47 blockade may have fewer effects than anticipated on normal T-lymphocyte trafficking and T-lymphocyte trafficking during acute injury and may be a more specific therapy for T-cell mediated inflammatory diseases of the intestine.
Trials of 60 GHz Radio for a Future 5G New Radio (NR) Solution for High Capacity CCTV Offload and Multimedia Transfer (2019)

This paper studies the radio interface performance of a 60 GHz radio in both indoor and outdoor conditions. The target is to assess its suitability for resolving emerging needs in the public transport, especially, in rail traffic, to transfer large amounts of data from vehicles to the stations and vice versa, during a short period of time. 60 GHz could also be ideal band for the wireless inter-carriage connection between the railcars. The related services and requirements are defined in the 5G specification Mobile Communication System for Railways TS22.289. 60 GHz band is also included in the 5G standard as an unlicensed band.
Combination of Small RNAs Enhances Muscle Development (2014)

Jackson, John R. PhD; Kim, NaJung PhD; Yoo, James MD, PhD; Lee, Sang Jin; Atala, Anthony MD, FACS Author Information
Relaxed threaded space-time codes (2005)

It is well-known that diversity, despite being widely used as a design criterion, may not be enough to ensure good performance of a wireless system, because the diversity factor may "kick in" at unrealistically high values of SNR. This paper proposes a new class of layered space-time codes with a new design criterion that works well in moderate SNR's. Specifically, we propose to relax some of the constraints of threaded algebraic space-time (TAST) codes, leading to a class of codes with better error performance, which we call relaxed threaded space time (RTST) codes. We also propose a modified design criterion, the average union bound (AUB), which ensures good performance at medium SNR. For a 2 times 2 system operating at 6 b/s/Hz, improvements of more than 1.5 dB have been observed