gebhart/patrepeval huggingface dataset:
3.75ppm/°C, -91dB PSRR, 27nW, 0.9V PVT Invariant Voltage Reference for Implantable Biomedical Applications (2020)

This brief presents a CMOS-only ultra low power voltage reference of 0.925V which is designed and simulated in TSMC 180nm technology. Current mixing between Self-Biased Self Cascode MOSFET (SBSCM) and Self Cascode MOSFET (SCM) pair is introduced along with stacking of proportional to absolute temperature (PTAT) and complementary to absolute temperature (CTAT) voltage generators to get second order curvature compensation. Temperature coefficient (TC) of 3.75ppm$/^{\circ}\mathrm{C}$ over a wide temperature range of $- 55^{\circ}\mathrm{C}$ to $140^{\circ}\mathrm{C}$ is achieved. To feed on the power reduction requirement of low power biomedical applications, the entire circuit is designed in sub-threshold region. Therefore, the total power consumption is 27nW @ 1.45V at room temperature $(27^{\circ}\mathrm{C})$. Furthermore, a supply independent current bias aids in realizing high PSRR of -91dB @ 10Hz / -50dB @ 100MHz and line regulation of 0.0779%/V over a supply range 1.45V to 3.6V. Besides, an employed trimming technique attenuates the process spread from ± 3% to ± 0.3%. Moreover, the proposed design occupies an active area of 0.0054mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> only.
Abnormal Expression of the B-Cell Homing Chemokine Receptor BLR1 During the Progression of Acquired Immunodeficiency Syndrome (1997)

The putative chemokine receptor BLR1 has been identified as the first G-protein–coupled receptor involved in B-cell migration and in microenvironmental homing to B-cell follicles and to germinal centers. In healthy individuals, expression of BLR1 is restricted to all mature recirculating B cells and to a subpopulation of T-helper memory cells. In the present study, we analyzed the distribution of BLR1 on defined lymphocyte subsets during the progression of acquired immunodeficiency syndrome. It is shown that the proportion of T-helper memory cells coexpressing BLR1 continuously decreases during the infection, whereas a high proportion of γ/δ T cells expressing BLR1 can be found in peripheral blood. The latter subpopulation is restricted to lymphoid tissues in healthy individuals. Most interestingly, in 75% of all human immunodeficiency virus (HIV)+ individuals, peripheral blood B cells were identified as not expressing BLR1 and phenotypically resembling germinal center cells of lymphoid tissue. Using BLR1 as a marker molecule, this study identifies peripheral blood lymphocytes in HIV+ individuals that are usually restricted to lymphoid tissue in healthy individuals. Because HIV infection is active in lymphoid tissue even at the clinically latent stage, aberrant expression of the B-cell homing chemokine receptor BLR1 might be an early indicator for the onset of destruction of lymphoid tissue.
Molecular Insights into the Thermal Stability of mAbs with Variable‐Temperature Ion‐Mobility Mass Spectrometry (2015)

The aggregation of protein-based therapeutics such as monoclonal antibodies (mAbs) can affect the efficacy of the treatment and can even induce effects that are adverse to the patient. Protein engineering is used to shift the mAb away from an aggregation-prone state by increasing the thermodynamic stability of the native fold, which might in turn alter conformational flexibility. We have probed the thermal stability of three types of intact IgG molecules and two Fc-hinge fragments by using variable-temperature ion-mobility mass spectrometry (VT-IM-MS). We observed changes in the conformations of isolated proteins as a function of temperature (300-550 K). The observed differences in thermal stability between IgG subclasses can be rationalized in terms of changes to higher-order structural organization mitigated by the hinge region. VT-IM-MS provides insights into mAbs structural thermodynamics and is presented as a promising tool for thermal-stability studies for proteins of therapeutic interest.
Supplementary Table 1 from CD16<sup>+</sup>NKG2A<sup>high</sup> Natural Killer Cells Infiltrate Breast Cancer–Draining Lymph Nodes (2023)

&lt;p&gt;Supplementary Table 1&lt;/p&gt;
Toolstring Impact Sensors (2007)

Abstract Since the application of slickline technology for wellbore intervention, the need for a measurement device to determine not only strain on the wire but forces applied to the toolstring at depth has been required. What stands between a successful intervention and a ‘miss-run’ is the skill of the slickline operator and the surface weight indicator known as a load cell. This paper will address the lesson learned from applying ‘Impact Sensors’ to slickline toolstrings in over 100 applications since it's launch in September 2005. The paper will also cover a case study that utilized the technology to better understand downhole conditions and their effects on toolstrings of various geometries. Completion technology has altered greatly and the results of directional drilling technology and horizontal completion architecture have proven to enhance the delivery of hydrocarbons to surface. The application of new technology has been implemented by the Electric Line, Coil Tubing, Drilling, Reservoir Analysis and other groups in the industry, however the application of new technology to the slick line intervention business has been greatly lacking and because of this many completion engineers develop completion installation programs with the least amount of wire in the hole as possible. Much of this reluctance is due to the perceived risk associated with deploying slick line into the well bore where an ‘unknown event’ may cause irreparable damage to the completion system or at the very least a costly exercise in equipment recovery. The application of impact sensors to the slick line toolstring eliminates much of associate unknown risk factors by supplying the operator with known parameters being applied at depth.
A locus conferring effective late blight resistance in potato cultivar Sárpo Mira maps to chromosome XI (2013)

Late blight of potato, caused by Phytophthora infestans, is one of the most economically important diseases worldwide, resulting in substantial yield losses when not adequately controlled by fungicides. Late blight was a contributory factor in The Great Irish Famine, and breeding for resistance to the disease began soon after. Several disease-resistant cultivars have subsequently been obtained, and amongst them Sárpo Mira is currently one of the most effective. The aim of this work was to extend the knowledge about the genetic basis of the late blight resistance in Sárpo Mira and to identify molecular markers linked to the resistance locus which would be useful for marker-assisted selection. A tetraploid mapping population from a Sárpo Mira × Maris Piper cross was phenotyped for foliar late blight resistance using detached leaflet tests. A locus with strong effect on late blight resistance was mapped at the end of chromosome XI in the vicinity of the R3 locus. Sárpo Mira's genetic map of chromosome XI contained 11 markers. Marker 45/XI exhibited the strongest linkage to the resistance locus and accounted for between 55.8 and 67.9% of variance in the mean resistance scores noted in the detached leaflet assays. This marker was used in molecular marker-facilitated gene pyramiding. Ten breeding lines containing a late blight resistance locus from cultivar Sárpo Mira and the Rpi-phu1 gene originating from the late blight resistant accession of Solanum phureja were obtained. These lines have extended the spectrum of late blight resistance compared with Sárpo Mira and it is expected that resistance in plants containing this gene pyramid will have enhanced durability.
Identification of new liver tumor biomarkers using proteomics. (2012)

e21091 Background: It is crucial to identify new biomarkers that help diagnose cancers at an early stage and contribute to the development of new anti-cancer drugs. Here, we identify new liver tumor biomarkers using proteomics. Methods: We evaluated 9 types of liver tissue from 55 patients: normal liver parenchyma (n=7), hepatocellular carcinoma (HCC) (n=7), normal bile duct (n=6), peripheral cholangiocarcinoma (CC) (n=7), hilar CC (n=7), CC associated with primary sclerosing cholangitis (PSC) (n=7), metastatic colorectal cancer (n=7), and mixed HCC/CC after trans-arterial chemoembolization (n=7; areas of HCC and areas of CC separately examined). Protein extracted from microdissected, formalin-fixed paraffin-embedded tissue (0.15 mm 3 in total) was reduced, alkylated and digested with trypsin in a stacking gel. Peptides were analyzed using nano-liquid chromatography-Mass spectrometry. The raw data were searched using Mascot. Normalized spectral counts for each protein among each tissue type were compared. For each comparison, an unrelated t-test was computed to obtain the p value. q values (adjusted p values) were computed using a direct False Discovery Rate approach (q &lt; 0.05 considered statistically significant). Results: The mean number of proteins identified per sample was 762 +/- 119 S.D, resulting in a total of 2643 proteins identified. Numbers of proteins with significantly different expression levels among different tissue types are shown below. Some of these proteins are being reported here for the first time in the context of liver carcinogenesis. Conclusions: This study revealed proteins that are significantly over/underexpressed in particular types of liver tumors. Clinically useful new biomarkers may be present among these proteins and are now undergoing validation using immunohistochemisty. [Table: see text]
Figure S5 from Human CD4<sup>+</sup> T Cells Specific for Merkel Cell Polyomavirus Localize to Merkel Cell Carcinomas and Target a Required Oncogenic Domain (2023)

&lt;p&gt;Minimal epitope and cross reactivity determination of WEDLT209-228 -specific CD4 T cells clones.&lt;/p&gt;
Thyroxine and insulin-like growth factor-I in milk and plasma of camels (Camelus dromedarius) (2002)

To study the pattern of insulin-like growth factor-1 (IGF-1) and thyroxine (T4) levels in four lactating camels and their newborns, by radioimmunoassay. Milk and blood samples were taken at parturition, and on the 1st, 2nd, 3rd, 7th, 14th, and 21st day after parturition. Milk IGF-1 levels were high at parturition and decreased with stages of factation, whereas T4 levels were low at delivery and progressively increased after. T4 levels in calves, were 4-5 times higher than those of their dams at birth, then declined thereafter but remained almost twice those in dams on day 21. In newborn calves, IGF-1 levels were significantly lower at birth compared to those of dams, and gradually increased with age until reached the same values of those in dams on day 21. These profiles were similar to those reported in domestic ruminants, and might be considered as preliminary data for dynamic studies of bone metabolism in camels. (Resume d'auteur)
The Distortion-Rate Function of Sampled Wiener Processes (2018)

We consider the recovery of a continuous-time Wiener process from a quantized or a lossy compressed version of its uniform samples under limited bitrate and sampling rate. We derive a closed-form expression for the optimal tradeoff among sampling rate, bitrate, and quadratic distortion in this setting. This expression is given in terms of a reverse waterfilling formula over the asymptotic spectral distribution of a sequence of finite-rank operators associated with the optimal estimator of the Wiener process from its samples. We show that the ratio between this expression and the standard distortion rate function of the Wiener process, describing the optimal tradeoff between bitrate and distortion without a sampling constraint, is only a function of the number of bits per sample. We also consider a sub-optimal lossy compression scheme in which the continuous-time process is estimated from the output of an encoder that is optimal with respect to the discrete-time samples. We show that the latter is strictly greater than the distortion under optimal encoding but only by at most 3%. We, therefore, conclude that near optimal performance is attained even if the encoder is unaware of the continuous-time origin of the samples.
Clinical applications of submucosal endoscopy (2011)

The SEMF procedure was originally developed to provide safe access to the peritoneal cavity for NOTES procedures. In the SEMF method, the submucosal layer is endoscopically tunneled and the resultant space is used as an endoscopic path into deeper layers of the gut wall and an offset exit to the peritoneal cavity. Key to the method is the overlying mucosa which serves as a sealant flap minimizing the risk of soiling a body cavity with lumenal contents and the ease by which the entry point into the submucosal working space can be closed. Positive outcomes of the early SEMF clinical experiences validate that the submucosa can be transformed into a promising novel working space for flexible endoscopy.
Production of cell‐associated PDGF‐AA by a human sarcoma cell line: evidence for a latent autocrine effect (1996)

The alternative splicing of platelet-derived growth factor A-chain is known to result in 2 different protein products. One variant is encoded by transcripts containing the 69 nts representing exon 6 (PDGF-AAL), and one variant is encoded by transcripts in which exon 6 is excluded (PDGF-AAs). Transfection assays have suggested that the long splice variant of the A-chain is mainly associated with membrane- and matrix-associated heparan sulphate proteoglycans, whereas the shorter variant is soluble. We describe a human sarcoma cell line (U-2197) that expresses a high level of PDGF-A transcripts. Immunoprecipitations revealed cell-associated protein products of mainly 24, 28 and 33 kDa and less abundant forms of 4—45 kDa, while no PDGF was found in the medium. Analysis of extracellular medium in a radioreceptor assay confirmed that PDGF was not secreted by the U-2 197 cells. The addition to U-2 197 cultures of a carboxy terminal peptide that specifically competes with the binding of the long splice variant of PDGF-AA to extracellular matrix and cell membranes resulted in the release of 3 PDGF-AA-specific dimeric proteins with molecular masses of 33, 37 and 45 kDa. Furthermore, polymerase chain reaction studies discriminating between the long and the short splice variants of the PDGF-A transcripts revealed that U-2197 expressed relatively higher amounts of the long splice variant compared with U-343 MGa C1 2:6, which is known to secrete PDGF-AA. These cell-associated forms of PDGF, released to the medium by adding carboxy terminal peptide, increased the tyrosine kinase activity of the endogenous PDGF α-receptor. © 1996 Wiley-Liss, Inc.
[Therapeutic angiogenesis for ischemic diseases]. (2006)

The clinical consequences of peripheral arterial disease include pain on walking, pain at rest and loss of tissue integrity in the distal ischemic limbs. Although development of beneficial drugs and intervention devices do contribute to the treatment of this disease, critical limb ischemia is estimated to develop in 500 to 1,000 individuals per million per year. As angiogenic growth factors can stimulate the development of collateral arteries, a concept called "therapeutic angiogenesis" is now evaluated in the clinical fields. Recent progress in molecular biology has led to the development of gene therapy as a new strategy to treat a variety of cardiovascular diseases using angiogenic growth factors such as vascular endothelial growth factor (VEGF). Therapeutic angiogenesis using angiogenic growth factors is expected to be a new treatment for patients with severe ischemic heart or peripheral arterial disease.
Supercomputing power for interactive visualization. Progress report, Oct 90-Mar 91 (1991)

The authors work over the past several years has explored multicomputer architectures for three dimensional graphics that offer the promise of scalability, very high performance, and greater flexibility and range of application than today's systems. While current high performance commercial graphics systems employ various forms of parallelism to support interaction with 3D objects and scenes, all operate on a single stream of graphics primitives. Much of our effort has been devoted to finding ways to operate in parallel both on lists of graphics primitives as well as on the pixels within primitives, and to incorporate parallel execution of user application code to generate and modify the graphics primitives to be displayed.
S-Nitrosylated Human Serum Albumin-mediated Cytoprotective Activity Is Enhanced by Fatty Acid Binding (2008)

Binding of oleate to S-nitrosylated human serum albumin (SNO-HSA) enhances its cytoprotective effect on liver cells in a rat ischemia/reperfusion model. It enhances the antiapoptotic effect of SNO-HSA on HepG2 cells exposed to anti-Fas antibody. To identify some of the reasons for the increased cytoprotective effects, additional experiments were performed with glutathione and HepG2 cells. As indicated by 5,5'-dithiobis-2-nitrobenzoic acid binding, the addition of oleate increased the accessibility of the single thiol group of albumin. Binding of increasing amounts of oleate resulted in increasing and more rapid S-transnitrosation of glutathione. Likewise, binding of oleate, or of a mixture of endogenous fatty acids, improved S-denitrosation of SNO-HSA by HepG2 cells. Oleate also enhanced S-transnitrosation by HepG2 cells, as detected by intracellular fluorescence of diaminofluorescein-FM. All of the S-transnitrosation caused by oleate binding was blocked by filipin III. Oleate also increased, in a dose-dependent manner, the binding of SNO-HSA labeled with fluorescein isothiocyanate to the surface of the hepatocytes. A model in two parts was worked out for S-transnitrosation, which does not involve low molecular weight thiols. Fatty acid binding facilitates S-denitrosation of SNO-HSA, increases its binding to HepG2 cells and greatly increases S-transnitrosation by hepatocytes in a way that is sensitive to filipin III. A small nitric oxide transfer takes place in a slow system, which is unaffected by fatty acid binding to SNO-HSA and not influenced by filipin III. Thus, fatty acids could be a novel type of mediator for S-transnitrosation.
The Rpi‐blb2 gene from Solanum bulbocastanum is an Mi‐1 gene homolog conferring broad‐spectrum late blight resistance in potato (2005)

The necessity to develop potato and tomato crops that possess durable resistance against the oomycete pathogen Phytophthora infestans is increasing as more virulent, crop-specialized and pesticide resistant strains of the pathogen are rapidly emerging. Here, we describe the positional cloning of the Solanum bulbocastanum-derived Rpi-blb2 gene, which even when present in a potato background confers broad-spectrum late blight resistance. The Rpi-blb2 locus was initially mapped in several tetraploid backcross populations, derived from highly resistant complex interspecific hybrids designated ABPT (an acronym of the four Solanum species involved:S. acaule, S. bulbocastanum, S. phureja and S. tuberosum), to the same region on chromosome 6 as the Mi-1 gene from tomato, which confers resistance to nematodes, aphids and white flies. Due to suppression of recombination in the tetraploid material, fine mapping was carried out in a diploid intraspecific S. bulbocastanum F1 population. Bacterial artificial chromosome (BAC) libraries, generated from a diploid ABPT-derived clone and from the resistant S. bulbocastanum parent clone, were screened with markers linked to resistance in order to generate a physical map of the Rpi-blb2 locus. Molecular analyses of both ABPT- and S. bulbocastanum-derived BAC clones spanning the Rpi-blb2 locus showed it to harbor at least 15 Mi-1 gene homologs (MiGHs). Of these, five were genetically determined to be candidates for Rpi-blb2. Complementation analyses showed that one ABPT- and one S. bulbocastanum-derived MiGH were able to complement the susceptible phenotype in both S. tuberosum and tomato. Sequence analyses of both genes showed them to be identical. The Rpi-blb2 protein shares 82% sequence identity to the Mi-1 protein. Significant expansion of the Rpi-blb2 locus compared to the Mi-1 locus indicates that intrachromosomal recombination or unequal crossing over has played an important role in the evolution of the Rpi-blb2 locus. The contrasting evolutionary dynamics of the Rpi-blb2/Mi-1 loci in the two related genomes may reflect the opposite evolutionary potentials of the interacting pathogens.
IDD 3 AND IDD 5 ALLELES FROM NOD MICE MEDIATE SJÖGREN'S SYNDROME-LIKE AUTOIMMUNITY. (2000)

Third International Conference on the Lacrimal Gland, Tear Film and Dry Eye Syndromes: Basic Science and Clinical Relevance: Maui, Hawaii; November 15-18, 2000: Abstracts
Nonhomogeneous distribution of human immunodeficiency virus type 1 proviruses in the spleen (1992)

A nonhomogeneous spatial distribution of human immunodeficiency virus type 1 proviruses in an infected spleen was observed. Antigenic stimulation of infected cells might explain this partition.
Rational Design of a Biomimetic Cell Penetrating Peptide Library (2013)

Cell penetrating peptides have demonstrated potential to facilitate the cellular delivery of therapeutic molecules. Here we develop a set of 50 cell penetrating peptide based formulations with potential to deliver small interfering RNAs intercellularly. The transfection efficacy of siRNA containing lipid-like nanoparticles decorated with different peptides was evaluated both in vitro and in vivo and correlated with the peptide physical and chemical properties. In vitro, these particles were internalized primarily through macropinocytosis. When the peptides were presented to bone marrow-derived dendritic cells, they induce low immunoactivation relative to control cell penetrating peptides including the antennapedia homeodomain and TAT, as quantified by the expression of activation specific surface proteins like CD80, CD86, and major histocompatibility complex class II. In vivo, peptide decorated nanoparticles primarily accumulated in the lungs and the liver. Three human peptides derived from surfactant protein B (a lung surfactant protein), orexin (a neuropeptide hormone, and lactoferricin (a globular glycoprotein) that exist in many physiological fluids facilitated the in vivo delivery of siRNA and induce significant knock down (90%) of a hepatocyte expressed protein, coagulation Factor VII.
Zinc tin oxide metal semiconductor field effect transistors and their improvement under negative bias (illumination) temperature stress (2017)

Metal-semiconductor-field-effect-transistors (MESFETs) with silver oxide Schottky gates on zinc tin oxide (ZTO) channels showed fundamental differences in stability compared to conventional amorphous-oxide semiconductor thin-film-transistors (AOS-TFTs). The most severe negative-bias-temperature and negative-bias-illumination-temperature stress conditions, which usually degrade the performance of AOS-TFTs, significantly improved the switching characteristic of these ZTO MESFETs, producing devices with on:off current ratios, mobilities, and subthreshold swings of 8 × 106, 12 cm2 V−1 s−1, and 180 mV/dec, respectively. Further analysis confirmed that both negative bias and temperature (65 °C) were simultaneously required to produce this permanent effect that was linked to the electromigration of ionized donors from the MESFET depletion-region.