gebhart/patrepeval huggingface dataset:
Low-dose morphine elicits ventilatory excitant and depressant responses in conscious rats: Role of peripheral &lt;i&gt;&#181;&lt;/i&gt;-opioid receptors (2013)

The systemic administration of morphine affects ventilation via a mixture of central and peripheral actions. The aims of this study were to characterize the ventilatory responses elicited by a low dose of morphine in conscious rats; to determine whether tolerance develops to these responses; and to determine the potential roles of peripheral μ-opioid receptors (μ-ORs) in these responses. Ventilatory parameters were monitored via unrestrained whole-body plethysmography. Conscious male Sprague-Dawley rats received an intravenous injection of vehicle or the peripherally-restricted μ-OR antagonist, naloxone methiodide (NLXmi), and then three successive injections of morphine (1 mg/kg) given 30 min apart. The first injection of morphine in vehicle-treated rats elicited an array of ventilatory excitant (i.e., increases in frequency of breathing, minute volume, respiratory drive, peak inspiratory and expiratory flows, accompanied by decreases in inspiratory time and end inspiratory pause) and inhibitory (i.e., a decrease in tidal volume and an increase in expiratory time) responses. Subsequent injections of morphine elicited progressively and substantially smaller responses. The pattern of ventilatory responses elicited by the first injection of morphine was substantially affected by pretreatment with NLXmi whereas NLXmi minimally affected the development of tolerance to these responses. Low-dose morphine elicits an array of ventilatory excitant and depressant effects in conscious rats that are subject to the development of tolerance. Many of these initial actions of morphine appear to involve activation of peripheral μ-ORs whereas the development of tolerance to these responses does not.
Continuous Microfluidic Synthesis of Pd Nanocubes and PdPt Core–Shell Nanoparticles and Their Catalysis of NO2 Reduction (2019)

Faceted colloidal nanoparticles are currently of immense interest due to their unique electronic, optical, and catalytic properties. However, continuous flow synthesis that enables rapid formation of faceted nanoparticles of single or multi-elemental composition is not trivial. We present a continuous flow synthesis route for the synthesis of uniformly sized Pd nanocubes and PdPt core-shell nanoparticles in a single-phase microfluidic reactor, which enables rapid formation of shaped nanoparticles with a reaction time of 3 min. The PdPt core-shell nanoparticles feature a dendritic, high surface area with the Pt shell covering the Pd core, as verified using high-resolution scanning transmission electron microscopy and energy dispersive X-ray spectroscopy. The Pd nanocubes and PdPt core-shell particles are catalytically tested during NO<sub>2</sub> reduction in the presence of H<sub>2</sub> in a flow pocket reactor. The Pd nanocubes exhibited low-temperature activity (i.e., <136 °C) and poor selectivity performance toward production of N<sub>2</sub>O or N<sub>2</sub>, whereas PdPt core-shell nanoparticles showed higher activity and were found to achieve better selectivity during NO<sub>2</sub> reduction retaining its basic structure at relatively elevated temperatures, making the PdPt core-shell particles a unique, desirable synergic catalyst material for potential use in NO<i><sub>x</sub></i> abatement processes.
Regulation of the Human Telomerase Gene TERT by Telomere Position Effect—Over Long Distances (TPE-OLD): Implications for Aging and Cancer (2016)

Telomerase is expressed in early human development and then becomes silenced in most normal tissues. Because ~90% of primary human tumors express telomerase and generally maintain very short telomeres, telomerase is carefully regulated, particularly in large, long-lived mammals. In the current report, we provide substantial evidence for a new regulatory control mechanism of the rate limiting catalytic protein component of telomerase (hTERT) that is determined by the length of telomeres. We document that normal, young human cells with long telomeres have a repressed hTERT epigenetic status (chromatin and DNA methylation), but the epigenetic status is altered when telomeres become short. The change in epigenetic status correlates with altered expression of TERT and genes near to TERT, indicating a change in chromatin. Furthermore, we identified a chromosome 5p telomere loop to a region near TERT in human cells with long telomeres that is disengaged with increased cell divisions as telomeres progressively shorten. Finally, we provide support for a role of the TRF2 protein, and possibly TERRA, in the telomere looping maintenance mechanism through interactions with interstitial TTAGGG repeats. This provides new insights into how the changes in genome structure during replicative aging result in an increased susceptibility to age-related diseases and cancer prior to the initiation of a DNA damage signal.
Soft Miniaturized Actuation and Sensing Units for Dynamic Force Control of Cardiac Ablation Catheters (2020)

Recently, there has been active research in finding robotized solutions for the treatment of atrial fibrillation (AF) by augmenting catheter systems through the integration of force sensors at the tip. However, limited research has been aimed at providing automatic force control by also integrating actuation of the catheter tip, which can significantly enhance safety in such procedures. This article solves the demanding challenge of miniaturizing both actuation and sensing for integration into flexible catheters. Fabrication strategies are presented for a series of novel soft thick-walled cylindrical actuators, with embedded sensing using eutectic gallium-indium. The functional catheter tips have a diameter in the range of 2.6-3.6 mm and can both generate and detect forces in the range of < 0.4 N, with a bandwidth of 1-2 Hz. The deformation modeling of thick-walled cylinders with fiber reinforcement is presented in the article. An experimental setup developed for static and dynamic characterization of these units is presented. The prototyped units were validated with respect to the design specifications. The preliminary force control results indicate that these units can be used in tracking and control of contact force, which has the potential to make AF procedures much safer and more accurate.
Synthesis of Nanoamorphous Germanium and Its Transformation to Nanocrystalline Germanium (2012)

A simple reaction between a mild reducing agent such as a trialkoxysilane and Ge(IV) species such as germanium tetraalkoxides in a room-temperature water/alcohol solution produces silica-coated ultrasmall (2-3 nm) amorphous germanium nanoparticles (na-Ge/SiO₂). The initial reaction involves the straightforward hydrolysis and condensation of the precursors, Ge(OCH₂ CH₃)₄ and (CH₃CH₂O)₃ SiH, where the reaction rate depends on the water concentration in the reaction medium. These processes can be further accelerated by adding acid to the reaction medium or carrying out the reaction at higher temperatures. At low water contents (up to 50% water/ethanol) and low acid concentrations, the reaction proceeds as a clear solution, and no precipitation is observed. The initially colorless clear solution progressively changes to pale yellow, yellow, orange, red, and finally dark red as the na-Ge particles grow. Evaporation of the solvent yields a reddish-brown powder/monolith consisting of na-Ge, embedded in an encapsulating amorphous silica matrix, na-Ge/SiO₂. The formation of na-Ge proceeds extremely slowly and follows a first-order dependence on both water concentration and diameter of the na-Ge particles under the reaction conditions used. Annealing of the na-Ge/SiO₂ powder under an inert atmosphere at 600 °C produces ultrasmall germanium nanocrystals (nc-Ge) embedded in amorphous silica (nc-Ge/SiO₂). Freestanding, colloidally stable nc-Ge is obtained by chemical etching of the encapsulating silica matrix.
ChemInform Abstract: (Pyridinylmethylidene)dithioles: Synthesis, Structure, and Reactivity. (1992)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Alterations of Respiratory Systems inAspergillus nigerunder the Conditions of Citric Acid Fermentation (1987)

Under the conditions of citric acid fermentation, Aspergillus niger WU-2223L possessed at least two respiratory systems. One system was sensitive to cyanide (CN) and the other was sensitive to salicylhydroxamic acid (SHAM). The respiration of mycelia was partially, inhibited by CN or SHAM, and was completely inhibited in the presence of both CN and SHAM. With culture time, the CN-sensitivity of respiration decreased and the SHAM-sensitivity increased. The respiratory rate itself decreased suddenly at 4 days and thereafter remained at a low level. The SHAM-sensitive respiration was localized in the mitochondria. The alterations of respiration in the mitochondria were similar to those observed in mycelial cells.
Multiple epitopes in a dodecapeptide of myelin basic protein determined by monoclonal antibodies. (1986)

Three custom synthesized myelin basic protein (MBP) peptides, bovine peptide 79-88, human peptide 80-89, and human peptide 82-91, were used to produce four murine monoclonal antibodies (MAb) that were selected on the basis of reaction in a solid phase radioimmunoassay (SRIA) with human MBP. The MAb were compared with respect to antigen specificity against intact MBP and 10 overlapping MBP peptides. One MAb recognized an epitope near the amino-terminus of bovine MBP peptide 79-88. A second MAb was directed towards an epitope that is more reactive in human MBP peptide 45-89 than in intact MBP, but is not recognized in any of the small MBP peptides examined. The third MAb detected an epitope near the middle of human MBP peptide 80-89, whereas the fourth MAb reacted with the carboxyl-terminal portion of human MBP peptide 82-91. Epitopes recognized in SRIA were sometimes not detected by the same MAb in a fluid phase double antibody radioimmunoassay. These results demonstrate the multiplicity of potential epitopes in a dodecapeptide of MBP and do not support the concept of a single, dominant epitope in the region of MBP peptide 80-89.
The agreement between inhaled mannitol airway challenge and exercise challenge in young children (2013)

Background: Inhaled mannitol and exercise challenge tests are used to assess exercise induced broncho-constriction in adults and school aged children. The forced oscillation technique (FOT) enables lung function and inhaled airway challenge tests in young children. There are limited reports of exercise challenge tests and no reports of mannitol challenge tests in young children with a history of exercise induced respiratory symptoms (EIS). Aims: To assess the agreement between mannitol and exercise challenge tests in young children using FOT. Methods: Healthy children and children with EIS were recruited. Children underwent a mannitol challenge and free running exercise challenge on 2 different visits 1-2 weeks apart. A positive responsiveness to the challenge tests was defined as wheeze on auscultation, persistent cough, SpO2 below 90% or increase in the respiratory resistance at 8 Hz (Rrs8) &gt;50% from control inhalations. Results: To date 37 children with a mean (range) age of 6 (4–7) years were enrolled of which 28 children performed both challenge tests. None of the healthy children (n=8) responded to either challenges. Of the 20 children with EIS 13 (65%) responded to the mannitol challenge with 8 children (40%) responding to the exercise challenge. In the children with EIS 7 children responded to both challenges and 6 children did not respond to either challenge resulting concordant outcomes in 13 children (65%) with EIS and in 100% of healthy children. Conclusion: These data suggest a mannitol challenge test combined with FOT may aid in the diagnosis of exercise induced asthma in young children and may offer an alternative to an exercise challenge test.
Visual Analytics of Volunteered Geographic Information: Detection and Investigation of Urban Heat Islands (2019)

Urban heat islands are local areas where the temperature is much higher than in the vicinity and are a modern phenomenon that occurs mainly in highly developed areas, such as large cities. This effect has a negative impact on energy management in buildings, and also has a direct impact on human health, especially for elderly people. With the advent of volunteered geographic information from private weather station networks, more high-resolution data are now available within cities to better analyze this effect. However, such datasets are large and have heterogeneous characteristics requiring visual-interactive applications to support further analysis. We use machine learning methods to predict urban heat islands occurrences and utilize temporal and spatio-temporal visualizations to contextualize the emergence of urban heat islands to comprehend the influencing causes and their effects. Subsequently, we demonstrate the analysis capabilities of our application by presenting two use cases.
Demonstration of uranium - plutonium separation and purification from spent nuclear fuel with monoamide solvent (2017)

International audience
A Novel Host-protein Assay Accurately Distinguishes Bacterial From Viral Upper Respiratory Tract Infections (2017)

Abstract Background Bacterial and viral infections are often clinically indistinguishable, particularly in upper respiratory tract infections (URTI), which leads to antibiotic misuse. A novel assay (ImmunoXpert™) that integrates measurements of three host-response proteins (TRAIL, IP-10, CRP) was recently developed to assist in differentiation between bacterial and viral etiologies. We evaluated the assay performance in URTI patients and compared it with standard laboratory measures. Methods We performed a sub-analysis of 464 patients with clinical suspicion of URTI enrolled in three previously conducted multi-center clinical studies that evaluated the assay performance in patients with acute infections: ‘Curiosity’ study (NCT01917461), ‘Opportunity’ study (NCT01931254), and ‘Pathfinder’ study (NCT01911143). Comparator method was predetermined criteria combined with expert panel adjudication, which was blinded to the test results. Diagnostic performance was evaluated by comparing test and comparator method outcomes. Results A unanimous panel adjudication was attained for 61 bacterial (13%) and 241 viral (52%) patients (162 patients (35%) had an indeterminate diagnosis). The assay distinguished between bacterial and viral infected patients with a sensitivity of 92% (95% CI: 82%- 98%) and specificity of 93% (88%-96%) with 11% equivocal test results. Overall the assay outperformed other routine laboratory tests (FIG 1), including: white blood cell count (WBC; cutoff 15,000 cells/µL, sensitivity 48% (35%-60%), P &amp;lt; 10-−6; specificity 85% (80%-90%), P &amp;lt; 0.05); CRP (cutoff 40 mg/L, sensitivity 82% (72%–92%), P = 0.16, specificity 79% (74%–84%), P &amp;lt; 10-4); Procalcitonin (PCT; cutoff 0.5 ng/mL, sensitivity 22% (11%–32%), P &amp;lt; 10–14, specificity 80% (74%–85%), P &amp;lt; 0.001); absolute neutrophil count (ANC; cutoff 10,000 cells/µL, sensitivity 58% (45%–71%), P &amp;lt; 10-−4, specificity 94% (91%–97%), P = 0.7). Conclusion The novel assay demonstrated superior performance compared with routine laboratory tests (WBC, ANC) and biomarkers (CRP, PCT), in distinguishing bacterial from viral etiologies in patients with URTI. It has the potential to help clinicians avoid missing bacterial infections or prescribing unwarranted antibiotics for viral URTIs. Disclosures K. Oved, MeMed Diagnostics: Board Member, Employee and Shareholder, Salary E. Eden, MeMed Diagnostics: Board Member, Employee and Shareholder, Salary T. Gottlieb, MeMed Diagnostics: Employee, Salary R. Navon, MeMed Diagnostics: Employee, Salary A. Cohen, MeMed Diagnostics: Employee, Salary O. Boico, MeMed Diagnostics: Employee, Salary M. Paz, MeMed Diagnostics: Employee, Salary L. Etshtein, MeMed Diagnostics: Employee, Salary G. Kronenfeld, MeMed Diagnostics: Employee, Salary T. Friedman, MeMed Diagnostics: Employee, Salary E. Bamberger, MeMed Diagnostics: Employee, Salary I. Chistyakov, MeMed Diagnostics: Consultant, Consulting fee I. Potasman, MeMed Diagnostics: Holding stock options, stock options
Ruthenium‐Catalyzed Biginelli Condensation: A Simple and Efficient One‐Pot Synthesis of 3,4‐Dihydropyrimidin‐2(1H)‐ones under Mild Reaction Conditions. (2006)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
On the operation of massive MIMO with and without transmitter CSI (2014)

The paper considers the issue of activating inactive terminals by control signaling in the downlink in a massive MIMO system. There are two basic difficulties with this. First, the lack of CSI at the transmitter. Second, the short coherence interval, which limits the number of orthogonal pilots in the case of many antennas. The proposed scheme deals with these issues by repeating the transmission over the antennas. We show that this repetition does not affect the spectral efficiency significantly, while making it possible to estimate the channel in a standard way using MMSE. The paper also sheds some light the uplink-downlink power balance in massive MIMO.
Journey Toward Process Convergence in TSV Technology (2010)

Continuous demand for more advanced electronic devices with higher functionality and superior performance in smaller packages is driving the semiconductor industry to develop new and more advanced 3D wafer-level interconnect technologies involving TSVs (through-silicon vias). The TSVs are created either on full-thickness wafer from the wafer front-side ¡V as part of wafer-fab processing during Middle-Of-Line (¡§via middle¡¨) or Back-End-Of-Line (¡§via last BEOL¡¨) ¡V or from the wafer backside after wafer thinning (¡§via last backside¡¨). Independent of the specific approach, the main steps include via etching, lining with insulator, copper barrier/seed deposition, via fill, and chemical mechanical planarization (CMP). Over the past year, the industry has been converging toward some primary unit processes and integration schemes for creating the TSVs. A common cost-of-ownership framework has also begun to emerge. Active collaboration underway among equipment suppliers, materials providers and end users is bringing about rapid development and validation of cost-effective TSV technology in end products. This presentation will address unit-process and integration challenges of TSV fabrication in the context of 20x100ƒÝm and 5x50ƒÝm baseline process flows at Applied Materials. Highlights of wafer-backside process integration involving wafers bonded to silicon or glass carriers will also be discussed.
Clinicopathologic Significance of the Mutations of theEpidermal Growth Factor ReceptorGene in Patients with Non–Small Cell Lung Cancer (2005)

These results in Japanese (East Asian) patients indicated that EGFR mutation plays an important role in pathogenesis of lung adenocarcinoma.
Traffic Jams Reduce Hydrolytic Efficiency of Cellulase on Cellulose Surface (2011)

A deeper mechanistic understanding of the saccharification of cellulosic biomass could enhance the efficiency of biofuels development. We report here the real-time visualization of crystalline cellulose degradation by individual cellulase enzymes through use of an advanced version of high-speed atomic force microscopy. Trichoderma reesei cellobiohydrolase I (TrCel7A) molecules were observed to slide unidirectionally along the crystalline cellulose surface but at one point exhibited collective halting analogous to a traffic jam. Changing the crystalline polymorphic form of cellulose by means of an ammonia treatment increased the apparent number of accessible lanes on the crystalline surface and consequently the number of moving cellulase molecules. Treatment of this bulky crystalline cellulose simultaneously or separately with T. reesei cellobiohydrolase II (TrCel6A) resulted in a remarkable increase in the proportion of mobile enzyme molecules on the surface. Cellulose was completely degraded by the synergistic action between the two enzymes.
In vivo inhibition of low density lipoprotein receptor–related protein improves survival of factor VIII in the absence of von Willebrand factor (2000)

We recently reported that administration of recombinant 39 kd receptor–associated protein (RAP) to von Willebrand factor (vWf) knockout mice induced a significant, sustained rise in endogenous murine factor VIII levels comparable to that induced by the administration of recombinant vWf.[1][1] RAP
Phylogenetic Distribution and Evolution of the Linked RNA-Binding and NOT1-Binding Domains in the Tristetraprolin Family of Tandem CCCH Zinc Finger Proteins (2014)

In humans, the tristetraprolin or TTP family of CCCH tandem zinc finger (TZF) proteins comprises 3 members, encoded by the genes ZFP36, ZFP36L1, and ZFP36L2. These proteins have direct orthologues in essentially all vertebrates studied, with the exception of birds, which appear to lack a version of ZFP36. Additional family members are found in rodents, amphibians, and fish. In general, the encoded proteins contain 2 critical macromolecular interaction domains: the CCCH TZF domain, which is necessary for high-affinity binding to AU-rich elements in mRNA; and an extreme C-terminal domain that, in the case of TTP, interacts with NOT1, the scaffold of a large multi-protein complex that contains deadenylases. TTP and its related proteins act by first binding to AU-rich elements in mRNA, and then recruiting deadenylases to the mRNA, where they can processively remove the adenosine residues from the poly(A) tail. Highly conserved TZF domains have been found in unicellular eukaryotes such as yeasts, and these domains can bind AU-rich elements that resemble those bound by the mammalian proteins. However, certain fungi appear to lack proteins with intact TZF domains, and the TTP family proteins that are expressed in other fungi often lack the characteristic C-terminal NOT1 binding domain found in the mammalian proteins. For these reasons, we investigated the phylogenetic distribution of the relevant sequences in available databases. Both domains are present in family member proteins from most lineages of eukaryotes, suggesting their mutual presence in a common ancestor. However, the vertebrate type of NOT1-binding domain is missing in most fungi, and the TZF domain itself has disappeared or degenerated in recently evolved fungi. Nonetheless, both domains are present together in the proteins from several unicellular eukaryotes, including at least 1 fungus, and they seem to have remained together during the evolution of metazoans.