gebhart/patrepeval huggingface dataset:
Regulation of Insulin Receptor Trafficking by Bardet Biedl Syndrome Proteins (2015)

Insulin and its receptor are critical for the regulation of metabolic functions, but the mechanisms underlying insulin receptor (IR) trafficking to the plasma membrane are not well understood. Here, we show that Bardet Biedl Syndrome (BBS) proteins are necessary for IR localization to the cell surface. We demonstrate that the IR interacts physically with BBS proteins, and reducing the expression of BBS proteins perturbs IR expression in the cell surface. We show the consequence of disrupting BBS proteins for whole body insulin action and glucose metabolism using mice lacking different BBS genes. These findings demonstrate the importance of BBS proteins in underlying IR cell surface expression. Our data identify defects in trafficking and localization of the IR as a novel mechanism accounting for the insulin resistance commonly associated with human BBS. This is supported by the reduced surface expression of the IR in fibroblasts derived from patients bearing the M390R mutation in the BBS1 gene.
Use of Novel Welding Technologies for High-Entropy Alloys Joining (2018)

Laser beam welding and friction stir welding of high entropy alloys (HEA) of the CoCrFeNiMn system were studied. The HEAs were produced by self-propagating high-temperature synthesis (SHS). Along with the principal elements, Al, C, S, and Si impurities were detected in the composition of the alloys. The as-cast alloys consisted of columnar fcc grains with coarse precipitates of MnS and fine Cr-rich M 23 C 6 carbides. Laser beam welding resulted in the formation of a defect-free weld joint. Precipitation of nanoscale B2 phase particles in the weld zone leaded to a pronounced increase in microhardness from ~150 HV of the base material to ~220 HV in the fusion zone. Friction stir welding (FSW) of a recrystallized state of the HEA with the average grain size of 3-5 μm resulted in the formation of a fine microstructure with a grain size of ~1.5 μm in the most strained area. Noticeable rise in strength and some decrease in ductility of the processed alloy in comparison with the initial condition can be associated with the formation of nanosized M 23 C 6 carbides.
Resonant optical devices for IR lasers (2013)

This paper highlights recent developments in resonant optical devices for infrared (IR) and mid-infrared (mid- IR) lasers. Sub-wavelength grating based resonant optical filters are introduced and their application in 2 &mu;m thulium fiber laser and amplifier systems has been discussed. The paper focuses on applying such filtering techniques to 2.8 &mu;m mid-IR fiber laser systems. A narrowband mid-IR Guided-Mode Resonance Filter (GMRF) was designed and fabricated using Hafnium(IV) Oxide film/quartz wafer material system. The fabricated GMRF was then integrated into an Erbium (Er)-doped Zr-Ba-La-Al-Na (ZBLAN) fluoride glass fiber laser as a wavelength selective feedback element. The laser operated at 2782 nm with a linewidth less than 2 nm demonstrating the viability of GMRF’s for wavelength selection in the mid-IR. Furthermore, a GMRF of narrower linewidth based on Aluminum Oxide/quartz wafer material system is fabricated and tested in the same setup. The potentials and challenges with GMRFs will be discussed and summarized.
Synthesis and Evaluation of the Antitumor Agent TMC‐69‐6H and a Focused Library of Analogues. (2005)

Abstract For Abstract see ChemInform Abstract in Full Text.
Cold Tolerance, SFR2, and the Legacy of Gary Warren (2004)

The beauty of a genetic approach to biological questions is that it can lead to the discovery of completely unexpected processes. This was a guiding precept for the research of Gary Warren, a molecular geneticist and prominent researcher in the field of cold tolerance at Royal Holloway College of
Meso-structural Control on Metal Surfaces Based on Powder Metallurgy and Its Application to Metal/Polymer Joining (2022)

This paper introduces meso-scale structure synthesis on metal surfaces through reaction processes from raw metal powders. The mesostructures are applied to metal/polymer anchor joining. The first topic is the synthesis of Fe-based porous structure on Fe substrate through the combustion reaction of the Fe-Ti-B system. The structure could be controlled in view of the phase equilibria at the maximum temperature of the reaction. A Fe/epoxy resin joint via the porous structure exhibits high tensile strength in a direction perpendicular to the joint interface. The second topic is the fabrication of mesostructure protruded on an Al alloy surface by laser scanning on Al-Ti-C powders. The meso-structural morphology is related to the formation of TiC and Al3Ti phases, which can be understood based on the solidification sequence after the laser irradiation. An Al alloy/CFRTP joint via the mesostructure exhibits high joint strength compared with previously reported similar anchor joints.
Abstract 480: Mammalian Organ Regeneration In Spiny Mice (2022)

Fibrosis-driven solid organ failure is a major global health burden. Spiny mice (genus: Acomys ) are terrestrial mammals that regenerate severe skin wounds without scars to avoid predation. To test whether spiny mice can also regenerate internal organ injuries, we performed acute obstructive or ischemic surgeries on adult kidneys of Acomys cahirinus compared to Mus musculus. Despite equivalent acute tissue damage, Acomys fully regenerate nephron structure, vascular &amp; lymphatic networks, and organ function without fibrosis by 14d while Mus (CD1 or C57BL/6) develop extensive interstitial fibrosis and progress to organ failure and death. Comparative transcriptomic, epigenetic, and single-cell assays revealed that the Acomys genome appears poised to initiate a regenerative response at the time of injury. In addition, we found the Hippo pathway effector protein YAP localized to the nucleus in renal tubular epithelial cells (RTEs) undergoing scarless wound healing in Acomys while it was either cytoplasmic or not detectable in Mus RTEs. Experiments in vitro revealed an accelerated PP2A-dependent YAP phosphatase activity that maintained nuclear YAP in Acomys RTEs and was not detected in Mus or human cells. Treatment of Acomys in vivo with the nuclear YAP-TEAD inhibitor verteporfin converted tissue regeneration to fibrosis in the dermis and similar experiments for the kidney are in progress. These results suggest that the spiny mouse genome evolved both transcriptional and post-translational adaptations important for scar-free regenerative wound healing in vivo .
Complex 3D-tailored facets for optimal lighting of facades and public places (2003)

Due to antiquated technologies (calculation methods, regulations, lighting and luminaire concepts, production techniques) current outdoor lighting causes a lot of problems like light pollution, glare, energy waste etc. New types of luminaires, and in consequence new outdoor lighting concepts, can be created by combining advanced calculation methods for optical surfaces with recent production technologies and novel light sources such as short arc metal halide lamps. Light emitted from this small Etendue light sources can precisely be redirected by 3D-curved surfaces manufactured with injection molding, milling and aluminium metallization. The required optical design may use techniques like complex surface calculations and 3D-Tailoring. An innovative concept based on the latest findings in visual perception research is to focus the light of such short arc light sources onto a facetted secondary mirror which provides the desired illuminance distribution on a facade or a public place. These systems are designed to fulfill lighting requirements as well as providing visual comfort. Thus lamps with improved color rendering, luminous efficacy and increased lifetime are used and glare is minimized by splitting the reflector into many facets (light spot decomposition). A few examples of realized projects will be presented where such complex facetted surfaces are used to reach a special quality of light. Using novel techniques like 3D-Tailoring, each facet can be designed to individually create the desired (e.g. uniform) illuminance distribution on the target surface - in this case, a large facade. For this particular application, we chose to impose a square boundary for each facet, in order to tile the rectangular aperture of the secondary mirror without compromising efficiency.
Alternative Lengthening of Telomeres Predicts Site of Origin in Neuroendocrine Tumor Liver Metastases (2014)

Alternative lengthening of telomeres was found to be a useful biomarker in patients with NELM. This marker can be helpful in guiding therapy by identifying the site of origin in patients in whom the primary site is unknown.
A 1.2-mW/Channel Pitch-Matched Transceiver ASIC Employing a Boxcar-Integration-Based RX Micro-Beamformer for High-Resolution 3-D Ultrasound Imaging (2023)

This article presents a low-power and small-area transceiver application-specific integrated circuit (ASIC) for 3-D trans-fontanelle ultrasonography. A novel micro-beamforming receiver architecture that employs current-mode summation and boxcar integration is used to realize delay-and-sum on an N -element sub-array using <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$N\times $ </tex-math></inline-formula> fewer capacitive memory elements than conventional micro-beamforming implementations, thus reducing the hardware overhead associated with the memory elements. The boxcar integration also obviates the need for explicit anti-aliasing filtering in the analog front end, thus further reducing die area. These features facilitate the use of micro-beamforming in smaller pitch applications, as demonstrated by a prototype transceiver ASIC employing micro-beamforming on sub-arrays of <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\textit {N}=4$ </tex-math></inline-formula> elements, targeting a wearable ultrasound device that monitors brain perfusion in preterm infants via the fontanel. To meet its strict spatial resolution requirements, a 10-MHz 100- <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\mu \text{m}$ </tex-math></inline-formula> -pitch piezoelectric transducer array is employed, leading to a per-element die area <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$&gt;$ </tex-math></inline-formula> 2 <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\times $ </tex-math></inline-formula> smaller than prior designs employing micro-beamforming.
Benzoxaborole as a new chemotype for carbonic anhydrase inhibition (2016)

In this paper we report the synthesis of a series of benzoxaborole derivatives, their inhibition properties against some carbonic anhydrases (CAs), recognized as important drug targets, and the characterization of the binding mode of these molecules to the CA active site. Our data provide the first experimental evidence that benzoxaboroles can be efficiently used as CA inhibitors.
An on-line character recognition system for effective Japanese input (2002)

An online character recognition system has been developed in order to allow effective Japanese entry via a pen interface. The system consists of handwriting recognition, contextual postprocessing, and enhanced Kana-to-Kanji conversion subsystems. The recognition subsystem uses a hybrid algorithm consisting of a pattern matching discriminator and a neural network verifier. Special care was taken to improve recognition of non-Kanji and simple Kanji characters frequently used in fast data entry. The postprocessor predicts consecutive characters on the basis of biagrams modified by the addition of parts of speech and substitution of macro characters for Kanji characters. A Kana-to-Kanji conversion method designed for ease of use with a pen interface is also integrated into the system. In an experiment in which 2499 samples of Kanji and non-Kanji characters were taken from 20 subjects, it was observed that the original recognition accuracy of 85% (the result obtained by using the pattern matching discriminator) was improved to 91.5% by adding the hybrid algorithm, and that it was further improved to 94.5% by adding the postprocessor. Improved recognition accuracy.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
413 Molecular dynamics simulation on the effect of the structure of water molecules on the contact angle on the platinum surface (2010)

Molecular dynamics simulation on the effect of the structure of water molecules
Enhancing Hematite-Based Invert Emulsion Mud Stability at High-Pressure High-Temperature Wells (2020)

Drilling fluids have a crucial continued role in drilling a successful well; however, most of the drilling technical and operational challenges are incorporated with the drilling mud stability and properties. The solid particles settling in drilling mud that deteriorates its stability is a common issue encountered in high-pressure high-temperature (HPHT) conditions. This issue, known as solids sagging, may eventually result in stuck pipes, wellbore instability, and loss of circulation. The objective of this work is to introduce garamite to enhance the stability of hematite-based invert emulsion mud under HPHT situations. The used garamite and hematite weighting material were analyzed using X-ray fluorescence, scanning electron microscopy, and particle size distribution to identify their compositions, morphologies, and particle sizes. The effects of adding different concentrations of garamite (0.5, 1.0, 1.25, and 1.5 g) to the field formula of hematite-based invert emulsion mud were investigated. The mud density, stability, sagging tendency, rheology, viscoelasticity, and filtration properties were studied to formulate a stabilized and distinguished-performance drilling mud. The obtained results indicated that garamite did not change the mud density while enhancing the emulsion stability by increasing the electrical stability proportionally with the added garamite quantity. The sagging experiments showed that adding 1.25 g of garamite is sufficient to prevent the sagging problem in both static and dynamic conditions as it was enough to enforce the sag parameters into the safe range of sag performance indicators. This 1.25 g of garamite improved the yield point by 152% from 19 to 48 lb./100 ft<sup>2</sup> with a slight increase in plastic viscosity from 14 cP for base mud to 18 cP and significant increase in the gelling strength and viscoelastic properties. Adding 1.25 g of garamite showed a slight enhancement in the filtration properties as the filtrate volume was reduced by 8% from 3.7 to 3.4 cm<sup>3</sup> and the filter cake thickness has 16% reduction from 2.69 to 2.26 mm. As a result, a mud with distinguished performance, in terms of rheology, suspension, sag performance, and stability, was obtained. Hence, a basis for safely drilling the HPHT formations was delivered, which reduces the drilling cost by minimizing the nonproductive time.
Funktionsorientierte Inline-Messtechnik von Faserverbunden/Function-oriented measurement technology of fibre composites – Integration of measurement results in FE simulations for functional evaluation of produced components (2019)

Hybride faserverstärkte Kunststoffe kombinieren die Vorteile ihrer unterschiedlichen Fasertypen und -materialien. Während des teuren Herstellungsprozesses kann es zu Fertigungsabweichungen kommen, deren Auswirkung auf die Bauteilfunktionalität unbekannt ist. Die Integration von Inline-Messtechnik zur quantitativen Erfassung der Abweichungen sowie die simulative Bewertung der Messergebnisse auf Funktionsebene während des Produktionsprozesses können den Ausschuss reduzieren und Kosten senken. &amp;nbsp; Hybrid fiber-reinforced plastics combine the advantages of their different fiber types and materials. During the expensive manufacturing process, production deviations with unknown influence on the part‘s performance may occur. The integration of inline measurement technology for quantitative measurement of the deviations and simulative evaluation of the measurement results at the functional level during the production process can reduce scrap and costs.
Transcriptional Activation of the Bacillus subtilis ackA Gene Requires Sequences Upstream of the Promoter (1998)

Transcriptional activation of the Bacillus subtilis ackA gene, encoding acetate kinase, was previously shown to require catabolite control protein A (CcpA) and sequences upstream of the ackA promoter. CcpA, which is responsible for catabolite repression of a number of secondary carbon source utilization genes in B. subtilis and other gram-positive bacteria, recognizes a cis-acting consensus sequence, designated cre (catabolite response element), generally located within or downstream of the promoter of the repressed gene. Two sites resembling this sequence are centered at positions -116.5 and -56.5 of the ackA promoter and have been termed cre1 and cre2, respectively. Synthesis of acetate kinase, which is involved in the conversion of acetyl coenzyme A to acetate, is induced when cells are grown in the presence of an easily metabolized carbon source such as glucose. In this study, cre2, the site closer to the promoter, and the region upstream of cre2 were shown to be indispensable for CcpA-dependent transcriptional activation of ackA, whereas cre1 was not required. In addition, insertion of 5 bp between cre2 and the promoter disrupted activation, while 10 bp was tolerated, suggesting face-of-the-helix dependence of the position of cre2 and/or upstream sequences. DNase footprinting experiments demonstrated binding of CcpA in vitro to cre2 but not cre1, consistent with the genetic data. Activation of ackA transcription was blocked in a ptsH1/crh double mutant, suggesting involvement of this pathway in CcpA-mediated transcriptional activation.
Improving systems of care during and after a pregnancy complicated by hyperglycaemia: A protocol for a complex health systems intervention (2020)

This study will provide insights into the impact of health systems changes in improving care for women with hyperglycaemia during and after pregnancy in a challenging setting. It will also provide detailed information on process measures in the implementation of such health system changes.
ChemInform Abstract: Asymmetric Synthesis of Conformationally Constrained cis‐1‐Amino‐1‐ phenylcyclohexan‐2‐ol. (1996)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Carbon Monoxide Activates PERK-Regulated Autophagy to Induce Immunometabolic Reprogramming and Boost Antitumor T-cell Function (2022)

Transient activation of ER stress with carbon monoxide drives mitochondrial biogenesis and protective autophagy that elicits superior antitumor T-cell function, revealing an approach to improving adoptive cell efficacy therapy.
Validation of multiple subject-specific finite element models of unicompartmental knee replacement (2013)

Accurate computer modelling of the fixation of unicompartmental knee replacements (UKRs) is a valuable design tool. However, models must be validated with in vitro mechanical tests to have confidence in the results. Ten fresh-frozen cadaveric knees with differing bone densities were CT-scanned to obtain geometry and bone density data, then implanted with cementless medial Oxford UKRs by an orthopaedic surgeon. Five strain gauge rosettes were attached to the tibia and femur of each knee and the bone constructs were mechanically tested. They were re-tested following implanting the cemented versions of the implants. Finite element models of four UKR tibiae and femora were developed. Sensitivity assessments and convergence studies were conducted to optimise modelling parameters. The cemented UKR pooled R(2) values for predicted versus measured bone strains were 0.85 and 0.92 for the tibia and femur respectively. The cementless UKR pooled R(2) values were slightly lower at 0.62 and 0.73 which may have been due to the irregularity of bone resections. The correlation of the results was attributed partly to the improved material property prediction method used in this project. This study is the first to validate multiple UKR tibiae and femora for bone strain across a range of specimen bone densities.
Biotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome (2017)

Interrogation of gene regulatory circuits in complex organisms requires precise tools for the selection of individual cell types and robust methods for biochemical profiling of target proteins. We have developed a versatile, tissue-specific binary in vivo biotinylation system in zebrafish termed biotagging that uses genetically encoded components to biotinylate target proteins, enabling in-depth genome-wide analyses of their molecular interactions. Using tissue-specific drivers and cell-compartment-specific effector lines, we demonstrate the specificity of the biotagging toolkit at the biochemical, cellular, and transcriptional levels. We use biotagging to characterize the in vivo transcriptional landscape of migratory neural crest and myocardial cells in different cellular compartments (ribosomes and nucleus). These analyses reveal a comprehensive network of coding and non-coding RNAs and cis-regulatory modules, demonstrating that tissue-specific identity is embedded in the nuclear transcriptomes. By eliminating background inherent to complex embryonic environments, biotagging allows analyses of molecular interactions at high resolution.
Can CO2Fertilization Enhance Phytoremediation of Organic Soil Contamination? (2013)

Low efficiency is a key problem confronting the development and application of phytoremediation technology. Based on political pressure to reduce CO2 emissions in China and the fact that CO2 is necessary for plant photosynthesis, the effects of captured CO2 fertilization on phytoremediation of soil di-(2-ethylhexyl) phthalate (DEHP) pollution by C3 plant (mung bean, Vigna radiata L.) and C4 plant (maize, Zea mays L.) were investigated. Results showed that DEHP pollution negatively affected the growth and rhizosphere environments of both plants. After CO2 fertilization, both plants had more biomass (aboveground, belowground, and total dry weight), higher alkaline phosphatase activity, and more microbes with DEHP tolerance in their rhizospheres. Superoxide dismutase activity in leaves of both plants decreased significantly. Microbial community composition in both rhizospheres changed. CO2 fertilization also increased plant uptake of DEHP, particularly in the roots, and decreased residual DEHP concentrations in the rhizospheres. These effects were more evident in the C3 than in the C4 plant. This study indicated that CO2 fertilization can enhance the phytoremediation process of polluted soil through promoting plant growth, improving the rhizosphere environment, and increasing plant uptake of DEHP, particular in a C3 plant. CO2 fertilization could be considered as a measure to enhance phytoremediation.