gebhart/patrepeval huggingface dataset:
Higher adaptive functioning and lower rate of psychotic comorbidity in married versus unmarried individuals with 22q11.2 deletion syndrome (2017)

22q11.2 deletion syndrome (22q11.2DS) is a relatively common genetic disorder. Due to improvement in pediatric care, affected individuals live into adulthood, some of whom marry or have committed relationships, and reproduce. The current study aimed to identify the factors that discriminate between married and unmarried adults with 22q11.2DS. In the presents study, 90 adults with 22q11.2DS (48 men/42 women), aged 29.8 ± 10.3 years, were included in the analysis. Psychiatric comorbidities, IQ score, and adaptive functioning were assessed using gold-standard diagnostic tools. Demographic factors, marital status, and reproductive status were evaluated by self-reports. Of the sample 25 adults (27.7%) were married and 14 (56%) of those had children. Married, as compared to unmarried individuals, were older, had less psychotic comorbidities, showed higher adaptive functioning in all domains of the Vineland Adaptive Behavior Scale, and had higher rates of independent living and sustained employment. Unexpectedly, married individuals showed higher rates of mood disorders and full scale IQ scores did not discriminate between the groups. We propose that multiple factors are associated with marital status among individuals with 22q11.2DS. Identification of key personal, functional, and social characteristics of those who married and reproduced may help counseling health professionals and clinicians in advising affected individuals and their families.
Proximity Gettering Design of Hydrocarbon–Molecular–Ion–Implanted Silicon Wafers Using Dark Current Spectroscopy for CMOS Image Sensors (2019)

We developed silicon epitaxial wafers with high gettering capability by using hydrocarbon-molecular-ion implantation. These wafers also have the effect of hydrogen passivation on process-induced defects and a barrier to out-diffusion of oxygen of the Czochralski silicon (CZ) substrate bulk during Complementary metal-oxide-semiconductor (CMOS) device fabrication processes. We evaluated the electrical device performance of CMOS image sensor fabricated on this type of wafer by using dark current spectroscopy. We found fewer white spot defects compared with those of intrinsic gettering (IG) silicon wafers. We believe that these hydrocarbon-molecular-ion-implanted silicon epitaxial wafers will improve the device performance of CMOS image sensors.
Control loop automation management platform (CLAMP) (2016)

This paper provides an overview of how intelligent services can be realized within the D2 architecture. An intelligent service collects information about resources (users, devices, networks and applications) used to deliver the service and about the environment in which it operates. It then makes decisions based on this information and domain knowledge which includes adapting and personalizing the service for the users consuming it. An intelligent service receives feedback on its performance and learns. There are primarily three attributes that characterize an intelligent service, known as PAD (personalized, adaptive, and dynamic). A predictive personalized service is one that anticipates a user's need and proactively takes intelligent actions and recommends valuable and timely personalized information. An adaptive service learns from its past actions of all users and adjusts its behavior to provide superior service quality. A service that is dynamic is one that is robust and is able to survive and self-repair or self-organize itself from possible service interruptions.
A New Bifidobacteria Expression SysTem (BEST) to Produce and Deliver Interleukin-10 in Bifidobacterium bifidum (2018)

In the last years there has been a growing interest in the use of genetically modified bacteria to deliver molecules of therapeutic interest at mucosal surfaces. Due to the well-recognized probiotic properties of some strains, bifidobacteria represent excellent candidates for the development of live vehicles to produce and deliver heterologous proteins at mucosal surfaces. However, very few studies have considered this genus because of its complexity to be genetically manipulated. In this work, we report the development of a new Bifidobacteria Expression SysTem (BEST) allowing the production of heterologous proteins in <i>Bifidobacterium bifidum</i>. This system is based on: i) the broad host range plasmid pWV01, ii) a stress-inducible promoter, and iii) two different signal peptides (SPs) one issued from <i>Lactococcus lactis</i> (SP<sub>Exp4</sub>) and issued from <i>Bifidobacterium longum</i> (SP<sub>BL1181</sub>). The functionality of BEST system was validated by cloning murine interleukin-10 (IL-10) and establishing the resulting plasmids (i.e., pBEST<sub>Exp4</sub>:IL-10 and pBEST<sub>BL1181</sub>:IL-10) in the strain of <i>B. bifidum</i> BS42. We then demonstrated <i>in vitro</i> that recombinant <i>B. bifidum</i> BS42 harboring pBEST<sub>BL1181</sub>:IL-10 plasmid efficiently secreted IL-10 and that this secretion was significantly higher (sevenfold) than its counterpart <i>B. bifidum</i> BS42 harboring pBEST<sub>Exp4</sub>:IL-10 plasmid. Finally, we validated <i>in vivo</i> that recombinant <i>B. bifidum</i> strains producing IL-10 using BEST system efficiently delivered this cytokine at mucosal surfaces and exhibit beneficial effects in a murine model of low-grade intestinal inflammation.
Supplementary Methods from Proteomic Characterization of the Angiogenesis Inhibitor SU6668 Reveals Multiple Impacts on Cellular Kinase Signaling (2023)

Supplementary Methods from Proteomic Characterization of the Angiogenesis Inhibitor SU6668 Reveals Multiple Impacts on Cellular Kinase Signaling
B-Cell Development and Differentiation During HIV Infection: Tolerance Versus Immunity (2010)

Early after HIV infection, B-cell homeostasis is disrupted which results in autoantibody production and auto- immunity. The systemic inflammation induced during chronic HIV infection relaxes B-cell mechanisms that normally suppress this autoimmune pathology. Tolerance to self antigens is established during the development of B cells; however, rare, broadly neutralizing antibodies to HIV cross-react with host cellular antigens, suggesting that mechanisms of B-cell limit protective humoral responses to HIV. The association between HIV infection, induction of autoantibody and the emergence of neutralizing antibodies directed at HIV suggests that protective humoral immune re- sponses may only occur after B-cell control mechanisms are relaxed. In this review, we focus on mechanisms that promote a diverse repertoire of antibody specificities during mouse and human B-cell development. Furthermore, we discuss mecha- nisms of that purge the B-cell repertoire of self- reactive clones and, as a consequence, limit the recognition of pathogens which express host-like antigens. In addition, we explore mechanisms that can be used by pathogens to subvert immune recognition, including the elicitation of autoantibody. We discuss the tolerance hypothesis of Hay- nes et al. (5) as a potential mode of immune evasion utilized by HIV to subvert neutralizing B-cell responses. B-CELL DEVELOPMENT
Steady‐State Behavior of Slurry and Bulk Propylene Polymerization (2003)

This paper analyzes the steady‐state behavior of two typical processes to produce poly(propylene): the single‐reactor bulk polymerization process and the slurry polymerization process in a train of continuous stirred tank reactors. The investigation is performed using a commercial process simulator—SIMULPOL 3.0®, and aims at elucidating the general behavior of both processes when subject to perturbations of the input operation variables. Attention is given to the effect of the perturbations upon the polymer properties and to identification of process bottlenecks. Regarding the bulk polymerization process, unstable plant behavior was detected in the absence of a control policy. It was found that multiple steady‐states are possible for this process as a consequence of the recycling of material. As a matter of fact, the reactor recycle flow rate was found to be a fundamental parameter for the bulk process. Unstable behavior can be eliminated with the implementation of classical control strategies.
Development of novel 18F-labeled PET tracers for imaging TSPO with insensitivity to rs6971 polymorphism (2020)

1047 Objectives: The translocator protein 18 kDa (TSPO), whose expression is upregulated by the activation of glia cells, is a continuously attractive biomarker for exploring neuroinflammation and neurologic disorders such as AD, PD and multiple sclerosis by PET. However, most TSPO PET tracers are sensitive to rs6971 polymorphism in human. In this study, we aim to identify some potential 18F-labeled PET tracers with high affinities, good brain uptake as well as reasonable insensitivity to rs6971 polymorphism.
 Methods: Through the structure-activity relationship study, two novel candidates with aryl fluorine moiety, namely LW-1-F and LW-2-F, were screened with high TSPO binding affinities. The corresponding 18F-isotopologues, 18F-LW-1 and 18F-LW-2, were synthesized automatically via spirocyclic iodonium ylide (SCIDY) strategy by CFN-MPS200 module. Dynamic neuroPET imaging were performed on wild-type Sprague-Dawley (SD) rats. The specificities and sensitivities to rs6971 polymorphism were evaluated by autoradiography (ARG) with LAB and HAB postmortem human brain slices. The pharmacokinetics were explored on mice by ex vivo biodistribution (bioD).
 Results: Both LW-1-F and LW-2-F showed excellent binding affinity with Ki 1 Ci/μmol) and radiochemical purities (> 99%). MicroPET imaging showed that 18F-LW-1 penetrated the blood-brain-barrier (BBB) of SD rat, with the highest whole brain uptake as 1.38 SUV at 2 min post-injection, and gradually washed out to 0.7 SUV after 20 min and remained at this level. The ARG results from human brain slices showed a certain level of sensitivity to rs6971 (ratioHAB/LAB = 1.7) and nonspecific binding. 18F-LW-2 showed reasonable insensitivity (ratioHAB/LAB = 1.1) and excellent in vitro specific binding by ARG study. Ex vivo biodistribution of 18F-LW-2 showed high radioactivity accumulation in the lungs, heart, kidney and adrenal glands, as well as urinary and hepatobiliary excretion.
 Conclusions: We have efficiently synthesized a series of novel TSPO ligands with aryl fluorine skeleton. Specifically, LW-1-F and LW-2-F showed good binding affinity to TSPO, which were successfully radiolabeled for imaging study. The microPET, in vitro ARG and ex vivo bioD study revealed that 18F-LW-2 showed good specificity, pharmacokinetics as well as reasonable insensitivity to rs6971 polymorphism, which is a potential PET tracer for visualizing TSPO in human.
Pressure and temperature stabilization of an existing chelle spectrograph II (2011)

The Echelle spectrograph FOCES,1 that was operated at the 2.2m Calar Alto telescope between 1995 and 2009 was moved to the laboratories of Munich University Observatories and is being as a test bed for a number of different stability issues related to high precision radial velocity spectroscopy. We utilize FOCES to study spectrograph stability, illumination stability and fiber transport stability. Results from temperature and pressure stabilization are presented with this paper. We will show, that we reach the requirements set by our model analysis approach presented in [2]. Peak to valley mid term stability of temperature and pressure is as good as 0.002K and 0.02hPa.
The Biosynthesis of Tropane Alkaloids in Datura stramonium: The Identity of the Intermediates between N-Methylpyrrolinium Salt and Tropinone (1997)

The biosynthesis of the tropane alkaloids in transformed root cultures of Datura stramonium has been studied using sodium [1,2-13C2]acetate, (R,S)-[2,3-13C2]-1-(1-methyl-2-pyrrolidinyl)propan-2-one {(R,S)-[2‘,3‘-13C2]hygrine}, ethyl (R,S)-[1,2-13C2,2-14C]-2-(1-methyl-2-pyrrolidinyl)acetate, and ethyl (R,S)-[2,3-13C2,3-14C]-4-(1-methyl-2-pyrrolidinyl)-3-oxobutanoate. The incorporation of (R,S)-[2‘,3‘-13C2]hygrine into cuscohygrine and several other condensation products was high (15−40% specific incorporation), but label was not recovered in either tropine or tropine esters (hyoscyamine; 0.0 ± 0.5% specific incorporation). None of the recovered alkaloids was labeled when ethyl (R,S)-[1,2-13C2,2-14C]-2-(1-methyl-2-pyrrolidinyl)acetate was fed to the cultures. In contrast, sodium [1,2-13C2]acetate and ethyl (R,S)-[2,3-13C2,3-14C]-4-(1-methyl-2-pyrrolidinyl)-3-oxobutanoate were incorporated into hyoscyamine (9 and 2% specific incorporation, respectively) and a number of other tropane alkaloids (up to 12% specific incorporation). These data provide further evidence that hygrine is not a direct precursor of tropane alkaloids. 13C-Label from acetate was incorporated symmetrically into the C-2 and C-4 positions of (−)-hyoscyamine. The evidence supports a pathway in which acetoacetate reacts via its C-4 position with N-methyl-Δ1-pyrrolinium salt to give 4-(1-methyl-2-pyrrolidinyl)-3-oxobutanoate. This intermediate favors cyclization to give 2-carboxytropinone, tropinone being formed by decarboxylation.
Efficient primary and parametric resonance excitation of bistable resonators (2016)

We experimentally demonstrate an efficient approach to excite primary and parametric (up to the 4th) resonance of Microelectromechanical system MEMS arch resonators with large vibrational amplitudes. A single crystal silicon in-plane arch microbeam is fabricated such that it can be excited axially from one of its ends by a parallel-plate electrode. Its micro/nano scale vibrations are transduced using a high speed camera. Through the parallel-plate electrode, a time varying electrostatic force is applied, which is converted into a time varying axial force that modulates dynamically the stiffness of the arch resonator. Due to the initial curvature of the structure, not only parametric excitation is induced, but also primary resonance. Experimental investigation is conducted comparing the response of the arch near primary resonance using the axial excitation to that of a classical parallel-plate actuation where the arch itself forms an electrode. The results show that the axial excitation can be more efficient and requires less power for primary resonance excitation. Moreover, unlike the classical method where the structure is vulnerable to the dynamic pull-in instability, the axial excitation technique can provide large amplitude motion while protecting the structure from pull-in. In addition to primary resonance, parametrical resonances are demonstrated at twice, one-half, and two-thirds the primary resonance frequency. The ability to actuate primary and/or parametric resonances can serve various applications, such as for resonator based logic and memory devices.
Erythromycin Biosynthesis:  Kinetic Studies on a Fully Active Modular Polyketide Synthase Using Natural and Unnatural Substrates (1996)

ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionErythromycin Biosynthesis: Kinetic Studies on a Fully Active Modular Polyketide Synthase Using Natural and Unnatural SubstratesRembert Pieper, Susanne Ebert-Khosla, David Cane, and Chaitan KhoslaCite this: Biochemistry 1996, 35, 31, 10248Publication Date (Web):August 6, 1996Publication History Published online6 August 1996Published inissue 1 January 1996https://pubs.acs.org/doi/10.1021/bi965010khttps://doi.org/10.1021/bi965010kcorrectionACS PublicationsCopyright © 1996 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views295Altmetric-Citations5LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (84 KB) Get e-Alertsclose Get e-Alerts
Real-time gene expression analysis in human xenografts for evaluation of histone deacetylase inhibitors (2006)

Real-time analysis of gene expression in experimental tumor models represents a major tool to document disease biology and evaluate disease treatment. However, monitoring gene regulation in vivo still is an emerging field, and thus far it has not been linked to long-term tumor growth and disease outcome. In this report, we describe the development and validation of a fluorescence-based gene expression model driven by the promoter of the cyclin-dependent kinase inhibitor p21waf1,cip1. The latter is a key regulator of tumor cell proliferation and a major determinant in the response to many anticancer agents such as histone deacetylase inhibitors. In response to histone deacetylase inhibitors, induction of fluorescence in A2780 ovarian tumors could be monitored in living mice in a noninvasive real-time manner using whole-body imaging. Single p.o. administration of the histone deacetylase inhibitor MS-275 significantly induces tumor fluorescence in a time- and dose-dependent manner, which accurately predicted long-term antitumoral efficacy in individual mice following extended treatment. These findings illustrate that this technology allows monitoring of the biological response induced by treatment with histone deacetylase inhibitors. In addition to providing experimental pharmacokinetic/pharmacodynamic markers for investigational drugs, this model provides insight into the kinetics of in vivo regulation of transcription, which plays a key role in causing and maintaining the uncontrolled proliferation of tumor tissue.
An atomic-scale nanoelectronic in - vivo sensing system: Fundamental limits (2008)

A self-contained or autonomous (10-¿m) <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sup> cubic system capable of migrating within a mammal to sense and communicate the in vivo state of individual cells is defined and analyzed to determine the fundamental limits for several component technologies. These component technologies include an embedded energy source, sensors, a control microprocessor, and a communication system. Interested in determining the limiting technology, we show that the communication system would consume most of the energy per bit or operation. This analysis suggests that while fabrication technologies needed to realize this system do not exist today and the tradeoffs are daunting, systems on this scale may be feasible using end-of-ITRS technologies.
Towards a school culture of pedagogical fairness: revisiting the academic performance of immigrant children in East Asia (2022)

Educational scholars have argued for fair pedagogical practices in response to the learning needs of diverse students. While pedagogical fairness has been widely advocated, few studies have systematically assessed its impact on student learning, and even fewer have examined pedagogical fairness from a school organisational perspective. To narrow this gap in research, the current study develops an expansive conceptualisation of pedagogical fairness as an integral part of organisational culture, which varies by school. Our data, gathered from 7,746 immigrant-background students attending 563 schools in six East Asian societies, were analysed based on a hierarchical linear model explaining their academic performance as a function of pedagogical fairness in terms of both teacher practice and school culture. The results suggest that fair pedagogy can effectively help immigrant children succeed in school. It is particularly notable that pedagogical unfairness embedded in school culture is negatively associated with the academic performance of immigrant children, even after controlling for unfair pedagogical practices exercised by individual teachers. These findings suggest that implementing fair pedagogy is not simply the responsibility of individual teachers; it is also the responsibility of school leaders, as they are in the position to substantially influence the school as an organisational whole.
Effects of sulfide on anaerobic digestion of primary and activatedbiosludge: A multi-approach study (2014)

Effects of sulfide on anaerobic digestion of primary and activatedbiosludge: A multi-approach study
Genome‐wide RNA‐ and MBD‐sequencing in HCT116 and DKO cells as a global re‐expression model (2012)

Short Abstract: Background: IT has been shown before that gene promoter methylation suppress expression of the downstream gene. This mechanism is involved in a series of diseases such as for example cancer, where tumor suppressor genes can be methylated and thus suppressed. \nMethodology: We did a genome-wide methylation analysis of a colon cancer cell line (HCT116) using Methyl-Binding-Domain (MDB) capturing followed by next-generation sequencing. We also did a genome-wide totalRNA (mRNA, miRNA, snoRNA ...) analysis of a colon cancer cell line (HCT116) and of a HCT116 cell line with both DNMT1 and DNMT3B knocked out (DKO) using directional next-generation RNA sequencing. Both the methylation and RNA were analysed using Illumina next-generation sequencing technology. \nResults: Using computational analysis and comparing the expression in HCT116 and DKO, and taking the methylation profile into account, we were able to identify pathways that are directly regulated by the methylation and pathways that are regulated in an indirect fashion. Furthermore, we were able to confirm known methylation regulated protein coding genes, miRNAs (e.g. hsa-mir-146a) and snoRNAs (e.g. SNORD116) in a single comprehensive sequencing experiment. We also could identify exons and alternative transcripts of which the expression is regulated by methylation, confirming the link between alternative splicing and methylation.