gebhart/patrepeval huggingface dataset:
METTL23 mutation alters histone H3R17 methylation in normal-tension glaucoma (2022)

Normal-tension glaucoma (NTG) is a heterogeneous disease characterized by retinal ganglion cell (RGC) death leading to cupping of the optic nerve head and visual field loss at normal intraocular pressure (IOP). The pathogenesis of NTG remains unclear. Here, we describe a single nucleotide mutation in exon 2 of the methyltransferase-like 23 (METTL23) gene identified in 3 generations of a Japanese family with NTG. This mutation caused METTL23 mRNA aberrant splicing, which abolished normal protein production and altered subcellular localization. Mettl23-knock-in (Mettl23+/G and Mettl23G/G) and -knockout (Mettl23+/- and Mettl23-/-) mice developed a glaucoma phenotype without elevated IOP. METTL23 is a histone arginine methyltransferase expressed in murine and macaque RGCs. However, the novel mutation reduced METTL23 expression in RGCs of Mettl23G/G mice, which recapitulated both clinical and biological phenotypes. Moreover, our findings demonstrated that METTL23 catalyzed the dimethylation of H3R17 in the retina and was required for the transcription of pS2, an estrogen receptor α target gene that was critical for RGC homeostasis through the negative regulation of NF-κB-mediated TNF-α and IL-1β feedback. These findings suggest an etiologic role of METTL23 in NTG with tissue-specific pathology.
Multiple complementary transcripts of pCMa1, a novel gene located at chromosome 11p15.1–2, and melanocytic cell transformation (2002)

The cDNA clone pCMa1 (0.45 kb) is one of the 12 novel cDNAs, previously identified when comparing RNA expression profiles of melanocytes, naevus cells, and non-metastatic melanoma cells. This clone did not reveal a unique long open reading frame. The pCMa1 gene localized to the distal, telomere proximal region on the short arm of chromosome 11.p15.1-2. Northern blot analyses with single-stranded cRNA probes revealed the presence of various complementary pCMa1 transcripts of different lengths, which are not enriched in the poly(A)(+) RNA fraction. The arbitrarily defined plus strand (used as a probe) mainly hybridized to 0.45 kb and 4.0 kb minus transcripts in total RNA samples, and the minus strand (used as a probe) hybridized to a major plus transcript of 4.0 kb. By RNA in situ hybridization, the highest levels of the plus transcripts were observed in melanocytic naevi (12/12), particularly in congenital naevi, whereas normal skin melanocytes (12/12) were negative. pCMa1 plus transcripts were detected in naevus cell nests (100%) near the dermo-epidermal junction. Expression, however, diminished to some extent in the deeper parts of the melanocytic naevi. Although most of the cutaneous primary melanoma lesions (11/15) showed detectable, but variable levels of plus transcripts of pCMa1 in the papillary to reticular dermis, not more than 10% of the melanoma cells were positive. The majority of melanoma metastases (6/7) were negative, while the positive lesion originated from a patient with a positive primary melanoma. Furthermore, plus transcripts were present in the nuclei of non-metastatic melanoma cells in culture, whereas metastatic cells showed elevated expression both in the nucleus and in the cytoplasm. Briefly, the data show transient up-regulation of pCMa1 in neoplastic progression of melanocytic cells, with peak levels occurring during naevus stages, and suggest that pCMa1 is a molecular marker in melanocytes for the early changes from the proliferating phenotype to malignant transformation.
De-endothelialized Rabbit Aorta CRITICAL ROLE OF NEOINTIMAL SMOOTH MUSCLE CELLS (1981)

A B S T R A C T Prostacyclin (PGI2) synthetic capacity was assayed at the surface of aortas at various intervals after removal of endothelium with a balloon catheter. Results were correlated with morphologic changes in the vessel wall seen by light microscopy, scanning and transmission electron microscopy. To assay PGI2 synthetic capacity, we applied an incubation chamber to the luminal surface ofthe aortas; after arachidonic acid stimulation we assayed the PGI2 synthesized with a bioassay and radioimmunoassay. PGI2 synthesis in deendothelialized aortas was determined immediately
Influence of Supply Voltage and Body Biasing on Single-Event Upsets and Single-Event Transients in UTBB FD-SOI (2021)

In this article, we present the effects of voltage scaling and forward body biasing (FBB) on single-event effect sensitivity of a 28-nm ultra-thin body and box (UTBB) fully depleted silicon on insulator (FD-SOI) technology. Heavy-ion irradiation was performed for single-event upset (SEU) and single-event transient (SET) sensitivity assessment on characterization test chips under three supply voltages, with and without back-gate voltage application. Measurements show a steady SEU sensitivity for any supply voltage across the two FBB configurations, whereas SET sensitivity is diminished under FBB. SPICE and TCAD mixed-mode simulations were run to assess the contribution of electrical factors as well as charge extraction mechanisms. While drive strength is increased under FBB, the bipolar amplification plays an important role in sensitivity at low linear energy transfers (LETs).
Highly Disordered Array of Silicon Nanowires: an Effective and Scalable Approach for Performing and Flexible Electrochemical Biosensors (2015)

The direct integration of disordered arranged and randomly oriented silicon nanowires (SiNWs) into ultraflexible and transferable electronic circuits for electrochemical biosensing applications is proposed. The working electrode (WE) of a three-electrode impedance device, fabricated on a polyimide (PI) film, is modified with SiNWs covered by a thin Au layer and functionalized to bind the sensing element. The biosensing behavior is investigated through the ligand-receptor binding of biotin-avidin system. Impedance measurements show a very efficient detection of the avidin over a broad range of concentrations from hundreds of micromolar down to the picomolar values. The impedance response is modeled through a simple equivalent circuit, which takes into account the unique WE morphology and its modification with successive layers of biomolecules. This approach of exploiting highly disordered SiNW ensemble in biosensing proves to be very promising for the following three main reasons: first, the system morphology allows high sensing performance; second, these nanostructures can be built via scalable and transferable fabrication methodology allowing an easy integration on non-conventional substrates; third, reliable modeling of the sensing response can be developed by considering the morphological and surface characteristics over an ensemble of disordered NWs rather than over individual NWs.
Inkjet-Printed Smart Skins and Wirelessly-Powered Sensors for Wearable Applications (2016)

This chapter discusses the design and fabrication process of inkjet-printed smart skins and wireless sensors. Complex multilayer structures can be implemented by stacking printed layers of conductive and dielectric inks, and sensing modules can be fabricated using nanomaterials such as graphene oxide and carbon nanotubes (CNTs). Microfluidic-tunable filters and antennas can be fabricated without the need of clean-room or chemical treatment, significantly reducing the manufacturing cost of such devices. The feasibility of making fully flexible circuits and sensors has been clearly demonstrated by either printing conductors or inkjet-printing detailed low-cost masks followed by simple etching techniques. Inkjet printing also facilitates the fabrication of structures for demanding scenarios such as microfludic sensors for biochemical applications. Finally, the easy realization of inkjet-printed energy harvesting circuits on flexible materials could potentially allow the full powering of microcontrollers and sensor circuitry, which would be necessary for the realization of truly autonomous wearable wireless systems for Internet of Things applications.
Reactivity of carboxy esters in gold–thiol monolayers (1993)

The reactivity of gold–thiol monolayers with carboxy ester functions at controlled distances from the surface is sensitive to this distance when this is highly ordered; such surfaces are inert to pig liver esterase, which accumulates, however, on both hydrophilic and hydrophobic surfaces.
Development of a novel immunotherapy for muscle invasive bladder cancer (MIBC). (2017)

402 Background: AGS-003 is an autologous tumor RNA-loaded dendritic cell-based immunotherapy being tested in advanced renal cell carcinoma (RCC) patients. The immunologic specificity for the patient’s own tumor is achieved using amplified tumor RNA electroporated into monocyte derived dendritic cells. To produce AGS-003 for RCC patients the RNA is amplified from a 100 mg primary tumor sample obtained via nephrectomy. In this non treatment study we evaluated the feasibility of RNA amplification from MIBC tissue acquired by transurethral resection of bladder tumor (TURBT). Methods: MIBC tissue was obtained via TURBT. Following draining of the irrigant used during the TURBT procedure, tissue was placed into an RNA preservative solution to prevent RNA degradation until processing. Methods developed for the amplification of RCC tumor RNA and RNA quality testing were applied to the RNA extracted from the MIBC specimens. Results: This work demonstrated that the methods developed for the amplification and testing of RNA from RCC can be successfully employed for the amplification of RNA from MIBC specimens collected by TURBT. Results demonstrated that the total RNA yields obtained from bladder tissue exceed that of similar masses of primary RCC tumors. This finding enabled the reduction in the amount of starting material from 100 mg to 50 mg of bladder tissue. The amount of amplified RNA produced from the lower bladder cancer masses were sufficient to support the production of immunotherapy at full scale (17 doses average). Conclusions: These data demonstrate the feasibility of RNA extraction and amplification from muscle invasive bladder cancer tissue and supports our planned phase II clinical study. [Table: see text]
Massive MIMO for Internet of Things (IoT) connectivity (2019)

Massive MIMO is considered to be one of the key technologies in the emerging 5G systems, but also a concept applicable to other wireless systems. Exploiting the large number of degrees of freedom (DoFs) of massive MIMO is essential for achieving high spectral efficiency, high data rates and extreme spatial multiplexing of densely distributed users. On the one hand, the benefits of applying massive MIMO for broadband communication are well known and there has been a large body of research on designing communication schemes to support high rates. On the other hand, using massive MIMO for Internet-of-Things (IoT) is still a developing topic, as IoT connectivity has requirements and constraints that are significantly different from the broadband connections. In this paper we investigate the applicability of massive MIMO to IoT connectivity. Specifically, we treat the two generic types of IoT connections envisioned in 5G: massive machine-type communication (mMTC) and ultra-reliable low-latency communication (URLLC). This paper fills this important gap by identifying the opportunities and challenges in exploiting massive MIMO for IoT connectivity. We provide insights into the trade-offs that emerge when massive MIMO is applied to mMTC or URLLC and present a number of suitable communication schemes. The discussion continues to the questions of network slicing of the wireless resources and the use of massive MIMO to simultaneously support IoT connections with very heterogeneous requirements. The main conclusion is that massive MIMO can bring benefits to the scenarios with IoT connectivity, but it requires tight integration of the physical-layer techniques with the protocol design.
Evaluation of the Prostate Cancer Prevention Trial Risk calculator in a high‐risk screening population (2009)

The PCPT risk calculator score was found to stratify prostate cancer risk in a cohort of young, primarily African-American men with a low baseline PSA level. These results support further evaluation of this predictive tool for assessing the risk of prostate cancer in high-risk men.
Electrogravimetric detection of DNA hybridization on polypyrrole copolymer (1998)

The immobilization of an oligonucleotide via a copolymerisation with a pyrrole monomer onto a Pt surface has been realized on a quartz crystal microbalance (QCM). The efficiency of this biosensor has been evaluated through the hybridization with different targets. Hybridization and adsorption responses were monitored in-situ by QCM frequency change.
Autoregulatory escape from norepinephrine infusion: roles of adenosine and histamine (1988)

Stimulation of sympathetic fibers or infusion of norepinephrine (NE) into the superior mesenteric artery (SMA) leads to an initial decrease in intestinal blood flow, which is followed by a return of flow toward the base-line value (autoregulatory escape) despite continued nerve stimulation or NE infusion. Although the mechanisms responsible for "autoregulatory escape" have not been defined, accumulation of vasodilator metabolites is frequently invoked to explain this phenomenon. Inasmuch as histamine and adenosine exist in high concentrations in the intestinal mucosa and both are potent vasodilators, we examined the effects of chlorpheniramine (an H1 blocker) and adenosine deaminase (degrades adenosine) on autoregulatory escape from NE infusion. In autoperfused piglet intestinal preparations, we measured SMA blood flow and the arteriovenous oxygen difference during intra-arterial NE infusion before and after blockade with chlorpheniramine or adenosine deaminase. Adenosine deaminase pretreatment increased the peak vasoconstrictor and reduced the steady-state escape responses to NE infusion. Chlorpheniramine did not affect either the vasoconstrictor or escape responses. The oxygen uptake changes induced by NE infusion were not dramatically modified by either treatment. These results indicate that adenosine but not histamine is responsible for at least part of the escape of intestinal blood flow from NE infusion.
Conformational Transitions at an S‐Layer Growing Boundary Resolved by Cryo‐TEM (2013)

Snapshots of growth: The use of a single graphene layer as the support for S-layer self-assembly of proteins provides an ideal, robust flat surface to image the expanding boundary by flash freezing and cryo-TEM, capturing the conformational states present at the expanding boundary (see figure). Image alignment and averaging provide a view of the steps leading to subunit recruitment and maturation in the self-assembly process. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Modeling percolation in high-aspect-ratio fiber systems. II. The effect of waviness on the percolation onset (2007)

The onset of electrical percolation in nanotube-reinforced composites is often modeled by considering the geometric percolation of a system of penetrable, straight, rigid, capped cylinders, or spherocylinders, despite the fact that embedded nanotubes are not straight and do not penetrate one another. In Part I of this work we investigated the applicability of the soft-core model to the present problem, and concluded that the hard-core approach is more appropriate for modeling electrical percolation onset in nanotube-reinforced composites and other high-aspect-ratio fiber systems. In Part II, we investigate the effect of fiber waviness on percolation onset. Previously, we studied extensively the effect of joint morphology and waviness in two-dimensional nanotube networks. In this work, we present the results of Monte Carlo simulations studying the effect of waviness on the percolation threshold of randomly oriented fibers in three dimensions. The excluded volumes of fibers were found numerically, and relationships between these and percolation thresholds for two different fiber morphologies were found. We build on the work of Part I, and extend the results of our soft-core, wavy fiber simulations to develop an analytical solution using the more relevant hard-core model. Our results show that for high- aspect-ratio fibers, the generally accepted inverse proportionality between percolation threshold and excluded volume holds, independent of fiber waviness. This suggests that, given an expression for excluded volume, an analytical solution can be derived to identify the percolation threshold of a system of high-aspect-ratio fibers, including nanotube-reinforced composites. Further, we show that for high aspect ratios, the percolation threshold of the wavy fiber networks is directly proportional to the analytical straight fiber solution and that the constant of proportionality is a function of the nanotube waviness only. Thus the onset of percolation can be adequately modeled by applying a factor based on fiber geometry to the analytical straight fiber solution.
Expression of the T4 molecule (AIDS virus receptor) by human brain‐derived cells (1987)

Three human cell lines of astrocytic origin were evaluated for expression of a human T-lymphocyte surface glycoprotein, T4, which also serves as a cellular receptor for the human immunodeficiency virus (AIDS virus, HIV). T4 antigen was detected on the cell surface of 2 of these cell lines using monoclonal OKT-4 antibody and flow cytometry. Gene transcripts encoding the T4 molecule were detected by a ribonuclease protection assay in surface T4-positive and -negative cells. Our results suggest that astrocytes may serve as targets for HIV infection in the brain.
Optically induced cross relaxation via nitrogen-related defects for bulk diamond C13 hyperpolarization (2017)

In this Rapid Communication we utilize nuclear magnetic resonance to investigate the hyperpolarization effect of negatively charged nitrogen vacancy (NV) centers on bulk $^{13}\mathrm{C}$ nuclei in a diamond single crystal. We were able to identify several polarization peaks of a different sign at different magnetic fields in a region of some tens of Gauss centered around $50\phantom{\rule{0.28em}{0ex}}\mathrm{mT}$. The bulk $^{13}\mathrm{C}$ hyperpolarization in the investigated field range is usually attributed to the excited state level anticrossing of the NV center. However, we found that this bulk hyperpolarization is caused by optically induced cross relaxation and that it takes place in the NV center ground state. The four-spin coupling between the polarized NV electron spin, the electron spin of a substitutional nitrogen impurity (P1), as well as its $^{14}\mathrm{N}$ nuclei and the $^{13}\mathrm{C}$ nuclear spin have to be considered. We introduce a simple theoretical model which completely fits with the experimental data and which clearly shows that the P1 centers are involved in the polarization process. We expect that the current work has a significant impact on future NV-based polarization applications.
PLL as the Frequency Synthesizer with Continuous Phase Divider (2009)

A PLL frequency synthesizer which (frequency) divider is replaced by a sinusoidal waveform generator has been analyzed. In the generator, n samples of the sinusoidal waveform are generated (created), and (while) the number of discrete points is equal to the dividing module of the divider. By this realization, within every VCO period one sampled value of the sinusoidal function has been generated, it has been sent to the phase detector and together with reference frequency it gives the control voltage. Subsequently, this allows faster reaching of the stable state, which implies high spectral purity of the sinusoidal waveform signal at the synthesizer's output. This approach keeps all advantages of the PLL synthesizers with look-in tables in terms of stability, and in the same time their architecture is much simpler. I
Semiconductor specialty technologies in IOT era (2015)

Summary form only given. Internet of Things (IOT) has caught a lot of attention recently due to tremendous business opportunities. In this talk, the speaker intends to give the historical figures in PC/ NB and smartphones at first, and then bring in the overall view of IOT. And then it will deploy to various semiconductor technology challenges and opportunities for IOT applications, including micro-controllers, micro-processors, wireless technologies, sensors, power managements, etc. The audience should expect to get clearer pictures of devices in IOT from this talk.
Activation of group II metabotropic glutamate (mGlu2/3) receptors regulates excitatory synaptic plasticity in the nucleus accumbens: Implications for the treatment of schizophrenia (2022)

Accumulating clinical evidence suggests that glutamatergic dysfunction may contribute to the pathogenesis of schizophrenia. Several studies have shown beneficial effects of group II metabotropic glutamate (mGlu 2/3 ) receptor agonists in rodents modeling schizophrenia symptomatology. However, the mechanisms through which mGlu 2/3 receptor agonists modulate glutamatergic neurotransmission in the nucleus accumbens (NAc) and schizophrenia‐like symptoms are still not clear. Therefore, we performed a series of experiments to evaluate the role of mGlu 2/3 receptors in regulating glutamatergic neurotransmission in the NAc. Using ex vivo whole‐cell patch‐clamp electrophysiology, we showed that bath application of mGlu 2/3 receptor agonist, LY379268 (100 nM), induces long‐term depression (LTD) of excitatory neurotransmission onto D1 medium spiny neurons (MSNs) in the NAc. Further, we evaluated the expression of mGlu 2/3 ‐LTD over the glutamatergic inputs from the midline nuclei of the thalamus (mThal) and basolateral amygdala (BLA) to the NAc. Interestingly, group II mGlu receptor agonist, LY379268, induces LTD of mThal‐NAc projections, but not BLA‐NAc projections, to D1 MSNs. Further, to test the hypothesis that mGlu 2/3 receptor activation can normalize glutamatergic neurotransmission in the NAc of mouse models of schizophrenia, we first measured spontaneous excitatory postsynaptic currents (sEPSCs) onto D1 MSNs of NAc in the slices prepared from the mice treated with either saline or MK801 (0.2 mg/kg; s.c ., 30 minutes). We found that acute treatment with MK801 enhances the frequency of spontaneous excitatory postsynaptic currents (sEPSCs) onto D1 MSNs. Subsequently, we examined the effect of group II agonist on MK801‐induced dysregulation of excitatory neurotransmission in the NAc. Excitingly, bath application of group II agonist, LY379268 (100 nM), reversed the MK801‐induced changes in sEPSCs in the NAc. Collectively, these data provide an evidence that activation of mGlu 2/3 receptors can rescue schizophrenia‐like pathophysiology in the NAc.