gebhart/patrepeval huggingface dataset:
Automating Quantum Dot Barcode Assays Using Microfluidics and Magnetism for the Development of a Point-of-Care Device (2013)

The impact of detecting multiple infectious diseases simultaneously at point-of-care with good sensitivity, specificity, and reproducibility would be enormous for containing the spread of diseases in both resource-limited and rich countries. Many barcoding technologies have been introduced for addressing this need as barcodes can be applied to detecting thousands of genetic and protein biomarkers simultaneously. However, the assay process is not automated and is tedious and requires skilled technicians. Barcoding technology is currently limited to use in resource-rich settings. Here we used magnetism and microfluidics technology to automate the multiple steps in a quantum dot barcode assay. The quantum dot-barcoded microbeads are sequentially (a) introduced into the chip, (b) magnetically moved to a stream containing target molecules, (c) moved back to the original stream containing secondary probes, (d) washed, and (e) finally aligned for detection. The assay requires 20 min, has a limit of detection of 1.2 nM, and can detect genetic targets for HIV, hepatitis B, and syphilis. This study provides a simple strategy to automate the entire barcode assay process and moves barcoding technologies one step closer to point-of-care applications.
Adenine nucleotide transport in hepatoma mitochondria and its correlation with hepatoma growth rates and tumor size. (1983)

Initial rates of [3H]adenosine diphosphate and [3H]adenosine triphosphate uptake were measured in mitochondria isolated from normal rat liver, regenerating liver, mouse hepatoma BW7756, and four Morris hepatomas (7777, 7800, 7794A, and 16) of varying degrees of malignancy. Results obtained demonstrate that (a) the apparent Km and Vmax values for adenosine diphosphate and adenosine triphosphate uptake are significantly lower in hepatoma compared to normal or regenerating liver mitochondria, (b) the Vmax values for adenosine diphosphate uptake correlate with tumor growth rate, and (c) the Km values for adenosine triphosphate in both hepatoma and normal mitochondria are lowered in the presence of added uncoupling agents; however, the extent of decrease is much less in fast-growing tumors than in slow-growing tumors and normal tissues. Studies examining the causes of reduced transport rates in hepatoma mitochondria showed that they are independent of the mitochondrial energy state and associated with substantially lower levels of the total and exchangeable adenine nucleotides. Additional studies revealed that transport rates are also dependent on the size of the tumor from which the mitochondria are isolated. Mitochondria isolated from small tumors (less than 2 g) had higher transport rates as well as higher levels of exchangeable and total adenine nucleotides than those isolated from larger tumors (4 to 6 g). Endogenous inhibitor levels also varied as a function of tumor size; free fatty acid levels increased, whereas acyl coenzyme A levels declined in mitochondria isolated from larger tumors. These results seem to indicate that, during the progression of tumor growth, mitochondria are experiencing cellular environmental changes that will affect overall tumor cell metabolism.
Hets att Lära för livet redan i Klass 9A : en historisk-kontextuell analys av tre omstridda skolskildringar (2014)

Uppsatsen utforskar några aspekter av de pedagogiska paradigm och ideologiska kontexter som födde, danade och formade tre av de senaste 70 årens otvivelaktigen mest omdiskuterade film- och tv-skildringar av den svenska skolan: Hets (film 1944), Lära för livet (tv-serie 1977), och Klass 9A (tv-serie 2008). Historierna bär tydliga spår av den kultur, tidsanda och samhällsdebatt de växte fram i, kom att alstras ur och slutligen absorberades av. De åskådliggör också i tur och ordning auktoritetsupproret, auktoritetens anonymisering och slutligen standardiseringen av den normaliserande auktoritetens vetande och tankefigurer.
Insights gained into activated sludge nitrification through structural and functional profiling of microbial community response to starvation stress (2019)

An improved understanding of nitrifying microbial communities in wastewater treatment is imperative for proper design and operation of biological nutrient removal systems.
Engineered Mesenchymal Stem Cell/Nanomedicine Spheroid as an Active Drug Delivery Platform for Combinational Glioblastoma Therapy (2019)

Mesenchymal stem cell (MSC) has been increasingly applied to cancer therapy because of its tumor-tropic capability. However, short retention at target tissue and limited payload option hinder the progress of MSC-based cancer therapy. Herein, we proposed a hybrid spheroid/nanomedicine system, comprising MSC spheroid entrapping drug-loaded nanocomposite, to address these limitations. Spheroid formulation enhanced MSC's tumor tropism and facilitated loading of different types of therapeutic payloads. This system acted as an active drug delivery platform seeking and specifically targeting glioblastoma cells. It enabled effective delivery of combinational protein and chemotherapeutic drugs by engineered MSC and nanocomposite, respectively. In an in vivo migration model, the hybrid spheroid showed higher nanocomposite retention in the tumor tissue compared with the single MSC approach, leading to enhanced tumor inhibition in a heterotopic glioblastoma murine model. Taken together, this system integrates the merits of cell- and nanoparticle- mediated drug delivery with the tumor-homing characteristics of MSC to advance targeted combinational cancer therapy.
Magnetoresistive double spin filter tunnel junction (2000)

We propose a magnetoresistive tunnel device that takes advantage of the spin filter effect. Two magnetic tunnel barriers are contacted by normal metal electrodes. The resistance of the device is lower (higher) when the magnetic moments of the two barriers are parallel (antiparallel). We present a theoretical calculation of the magnetoresistance. This device has the potential to work above room temperature, in very small fields, and to give a sensitivity orders of magnitude larger than what is possible with standard magnetic tunnel junctions.
VCP, a Weak ATPase Involved in Multiple Cellular Events, Interacts Physically with BRCA1 in the Nucleus of Living Cells (2000)

BRCA1, a breast/ovarian cancer susceptibility gene, undergoes mutations in as many as 50% of familial breast tumors. Recent studies indicate that BRCA1 may be involved in DNA damage repair. Here, we demonstrate that the BRCA1 protein physically associates with valosin-containing protein (VCP), a member of the ATPases associated with a variety of cellular activities (AAA) superfamily. In vitro studies revealed that VCP, via its N- terminal region, binds to amino acid residues 303-625 in the BRCA1 protein. Although found predominantly in the cytoplasm and, less abundantly, in the nucleus, VCP can be translocated from the nucleus after stimulation with epidermal growth factor. Collectively, our results suggest that VCP, by binding to BRCA1, participates in the DNA damage-repair function as an ATP transporter, possibly facilitating the transcription-coupled repair.
Hydraulic Remotely Adjustable Pulmonary Artery Banding (2010)

Each year approximately 2000 children are born with a form of congenital heart disease that would benefit from mechanical restriction, or banding, of the pulmonary artery. [1,2] Installing or changing the setup of the banding requires an open chest surgery and during the first 6 months of the patient’s life, physiological parameters evolve rapidly, resulting in need for frequent reoperations. Mortality for those treated patients may be as high as 10–20%. [3] While many devices have been patented, none of them have been adopted due to size, adjustability, or reliability constraints with regard to implantation in newborns, especially below 6 months of age. Here we present the conception, design, and scale model testing of a novel pulmonary banding system for infants. This system features a hydraulic mechanical stepper actuator that offers great advantages in both reliability and compactness. As a proof of concept, we built a 5:1 scale working prototype that demonstrated the desired functionality of the device. Further steps involve scaling down the device so first porcine trials can be started.
The putative role of cytokines in the induction of primary anti-phospholipid syndrome in mice (1992)

Antiphospholipid syndrome (APLS) is characterized by thrombocytopenia, thromboembolic phenomena and recurrent fetal loss, associated with anti-cardiolipin antibodies (ACA) and/or lupus anticoagulant. The syndrome may be primary or may be associated with other conditions such as systemic lupus erythematosus (SLE). In this study we induced primary APLS following immunization of BALB/c mice with a human monoclonal ACA (H-3). Analysis of the cytokine profile of the mice with experimental APLS indicated low production of IL-2, IL-3 and granulocyte-macrophage colony-stimulating factor (GM-CSF) by concanavalin A (Con A)-stimulated splenocytes of H-3 immunized mice. It seems that the low levels of IL-3 and GM-CSF have a potential role in the fetal loss of the APLS. Whatever the mechanism of IL-3 and GM-CSF in preventing fetal loss, these results may have therapeutic bearing on the reproductive outcome in women and other species with APLS.
Diabetogenic Action of GH and Cortisol in Insulin-Dependent Diabetes Mellitus Aspects of the Mechanisms Behind the Somogyi Phenomenon (1987)

The effect on glucose homeostasis of a transient elevation of plasma growth hormone (GH) and cortisol was studied over 6 h in 14 male patients with insulin-dependent diabetes mellitus (IDDM) by using an i.v. somatostatin (100 micrograms/h) - insulin (0.4 mU/kg/min) glucose (3 mg/kg/min) - infusion test (SIGIT). GH (20 mU/kg) was given as a 60 min i.v. infusion during the initial SIGIT period raising the plasma GH level to about 40 micrograms/l, and returning to low basal within 3 h. ACTH (0.1 mg) was given as an i.v. bolus injection at the start of the SIGIT, resulting in plasma cortisol peak values of about 900 nmol/l within 2-3 h. GH raised blood glucose after a lag of 4 h while ACTH alone had no effect. However, ACTH added to GH enhanced the diabetogenic effect of GH. It is concluded that an episodic increase in circulating GH-cortisol, resembling the responses of these hormones to an insulin-induced hypoglycemia, exerts a diabetogenic effect in IDDM-patients not deprived of insulin. While GH is essential in this respect the diabetogenic effect of cortisol is evident only in conjunction with GH.
Gaze distribution analysis and saliency prediction across age groups (2018)

Knowledge of the human visual system helps to develop better computational models of visual attention. State-of-the-art models have been developed to mimic the visual attention system of young adults that, however, largely ignore the variations that occur with age. In this paper, we investigated how visual scene processing changes with age and we propose an age-adapted framework that helps to develop a computational model that can predict saliency across different age groups. Our analysis uncovers how the explorativeness of an observer varies with age, how well saliency maps of an age group agree with fixation points of observers from the same or different age groups, and how age influences the center bias tendency. We analyzed the eye movement behavior of 82 observers belonging to four age groups while they explored visual scenes. Explorative- ness was quantified in terms of the entropy of a saliency map, and area under the curve (AUC) metrics was used to quantify the agreement analysis and the center bias tendency. Analysis results were used to develop age adapted saliency models. Our results suggest that the proposed age-adapted saliency model outperforms existing saliency models in predicting the regions of interest across age groups.
How reliable and robust are current biomarkers for copper status? – comments by Brewer and Althaus (2008)

We read the paper by Danzeisen et al. ( 1 ) with interest and would like to take issue with the authors on a number of topics.
Synchronous tree-adjoining grammars (1990)

The unique properties of tree-adjoining grammars (TAG) present a challenge for the application of TAGs beyond the limited confines of syntax, for instance, to the task of semantic interpretation or automatic translation of natural language. We present a variant of TAGs, called synchronous TAGs, which characterize correspondences between languages. The formalism's intended usage is to relate expressions of natural languages to their associated semantics represented in a logical form language, or to their translates in another natural language; in summary, we intend it to allow TAGs to be used beyond their role in syntax proper. We discuss the application of synchronous TAGs to concrete examples, mentioning primarily in passing some computational issues that arise in its interpretation.
Delay Efficient Load Balancing Scheme for Component Carrier Selection in Carrier Aggregation in LTE-A (2017)

In Long Term Evolution-Advanced (LTE-A), Carrier Aggregation(CA) allows user to simultaneously transmit and receive data on multiple carriers resulting in increased throughput. Carriers need to be selected to maximize load balancing and the spectral efficiency. In this paper, a load balancing CC se
24/7 Place Recognition by View Synthesis (2017)

We address the problem of large-scale visual place recognition for situations where the scene undergoes a major change in appearance, for example, due to illumination (day/night), change of seasons, aging, or structural modifications over time such as buildings being built or destroyed. Such situations represent a major challenge for current large-scale place recognition methods. This work has the following three principal contributions. First, we demonstrate that matching across large changes in the scene appearance becomes much easier when both the query image and the database image depict the scene from approximately the same viewpoint. Second, based on this observation, we develop a new place recognition approach that combines (i) an efficient synthesis of novel views with (ii) a compact indexable image representation. Third, we introduce a new challenging dataset of 1,125 camera-phone query images of Tokyo that contain major changes in illumination (day, sunset, night) as well as structural changes in the scene. We demonstrate that the proposed approach significantly outperforms other large-scale place recognition techniques on this challenging data.
Molecular dynamics simulations of the unfolding of apomyoglobin in water (1993)

Molecular dynamics simulations of apomyoglobin have been conducted in aqueous solution for 350 ps at 25 degrees C and for 500 ps in two different runs at 85 degrees C. The structures obtained at the higher temperature display properties similar to those of molten globules. Close agreement is obtained between the computed structural models and experimental data on the helical content of both native apomyoglobin and the low-pH unfolding intermediate. The results also suggest explanations for the surprising observations on the effects of mutations at the interface of the A, G, and H helices. Detailed analyses of the final structures and the unfolding pathways at high temperature clearly show that the most stable alpha-helical regions are those in contact with other helices.
Recycling of Crosslinked Polypropylene and Crosslinked Polyethylene in Supercritical Methanol (2012)

초임계 메탄올에서 가교폴리프로필렌의 탈가교 반응에 대해 연구하고 이를 가교폴리에틸렌의 탈가교 반응과 비교하였다. 회분식 반응기를 이용하여 반응 온도와 초기 겔 함량이 탈가교 반응 속도에 미치는 영향을 알아보았고, 정량적 분석을 위해 반응 전후의 겔 함량을 측정하였다. 반응 온도가 증가할수록, 초기 겔 함량이 증가할수록 탈가교 반응속도가 증가하였고, 반응 속도는 겔 함량에 선형적으로 의존하였다. 가교폴리프로필렌의 탈가교 반응이 가교폴리에틸렌보다 빠르게 일어나는 것을 확인하였고, 반응속도상수를 계산하여 이를 정량적으로 확인하였다. 이를 통해 재활용 측면에서는 가교폴리프로필렌이 가교폴리에틸렌보다 우수한 것을 알 수 있다. Decrosslinking of crosslinked polypropylene(XLPP) in supercritical methanol was investigated compared with that of crosslinked polyethylene(XLPE). Effect of reaction temperature and initial gel contents was studied using a batch reactor. Gel contents of samples before and after reaction were measured for quantitative analysis. As reaction temperature and initial gel contents increased, the decrosslinking rate increased. The rate of decrosslinking reaction is dependent linearly on the gel contents. Experimental results indicated that the decrosslinking of XLPP was faster than that of XLPE, and it was confirmed quantitatively by the calculation of kinetic constants. In conclusion, it is considered that XLPP has better recyclability than XLPE.
Chinese herbal medicine for functional constipation: a randomised controlled trial. (2013)

1. This was an 18-week prospective, randomised, double-blind, placebo-controlled clinical study on a Chinese herbal medicine--ma zi ren wan (MZRW)--for the treatment of functional constipation. 2. 120 subjects with functional constipation (Rome III criteria) were randomised (60 per arm) into the MZRW and placebo groups. Respective responder rates for the two groups were 43.3% and 8.3% during treatment, and 30.0% and 15.0% in the follow-up period (p<0.05). The MZRW group was superior to the placebo group in terms of increased complete spontaneous bowel movement as well as reduction in severity of constipation, straining at evacuation, and use of rescue therapy. No serious adverse effects were reported. 3. The dose of MZRW (7.5 g bid) was determined in a separate clinical trial. This study entailed a dose determination study and then a placebo-controlled clinical trial and can be a good reference for future studies.
Jodgehalte in Alleinfuttermitteln für Katzen in Deutschland (2003)

Zusammenfassung Um die Jodversorgung der Katze beurteilen zu können, wurde der Jodgehalt von 92 handelsüblichen, in Deutschland erhältlichen Alleinfuttermitteln für Katzen bestimmt. Es handelte sich um 74 Feuchtund 18 Trockenfuttermittel von 27 verschiedenen Herstellern. Zur Messung des Jodgehalts kam eine modifizierte Analysenmethode zum Einsatz, die auf einer alkalischen Trockenveraschung und der anschließenden eigentlichen Jodbestimmung nach Sandell und Kolthoff beruht. Bezogen auf die Trockensubstanz (TS) der Futtermittel lagen die Jodgehalte in einem Bereich von 218-6356 µg/kg TS und variierten damit um den Faktor 30. In Bezug auf die umsetzbare Energie (ME) ergab sich ein Jodgehalt von 11-290 µg/MJ ME. In den untersuchten Trockenfuttermittel (473-3181 µg/kg TS) unterlagen die Werte einer geringeren Streuung als in den Feuchtfuttermitteln (218-6356 µg/kg TS). Der durchschnittliche Jodgehalt betrug für die Trockenfuttermittel 1917 µg/kg TS und für die Feuchtfuttermittel 1942 µg/kg TS.
High-enthalpy, hypersonic compression corner flow (1996)

The results of an experimental investigation of high-enthalpy, hypersonic flow over sharp leading-edge compression corners are presented and discussed. In particular, the possible effects of real gas behavior are examined. Measurements have been made of the heat transfer and pressure distributions for flat plate and compression corner flow. Some flow visualization data have also been obtained. Test flows were generated using a free-piston shock tunnel operating in the reflected mode. The reservoir enthalpy ranged from 3 to 19 MJ kg -1 , giving freestream speeds of 2.3-5.5 km s -1 . For these conditions, the flow remains laminar throughout. The flat plate data for both high- and low-enthalpy flows are in agreement with the reference enthalpy method for heat transfer and the weak interaction theory for pressure. Also, the measured flat plate boundary-layer thickness compares well with an expression strictly valid for perfect gas flows only. The high- and low-enthalpy compression corner flows have upstream influence and plateau pressure behavior similar to perfect gas flow. That is, real gas effects for the present flows appear to be negligible. This is consistent with the essentially chemically frozen viscous and inviscid flow upstream of the interaction.
Al-2.127 Spin Coating (2021)

As increasingly smaller molecular materials and material structures are devised or developed for technological applications, the demands on the processes of lithography now routinely include feature sizes that are of the order of 10 nm. In reaching such a fine level of resolution, the methods of lithography have increased markedly in sophistication and brought into play 2terminology that is unfamiliar, on the one hand, to scientists tasked with the development of new lithographic materials or, on the other, to the engineers who design and operate the complex equipment that is required in modern-day processing. Publications produced by scientists need to be understood by engineers and vice versa, and these commonly arise from collaborative research that draws heavily on the terminology of two or more of the traditional disciplines. It is developments in polymer science and material science that lead progress in areas that cross traditional boundaries, such as microlithography. This document provides the exact definitions of a selection of unfamiliar terms that researchers and practitioners from different disciplines might encounter.
Fiber-chip link via mode division multiplexing (2023)

Applying mode division multiplexing techniques to a fiber-chip link would greatly increase its communication bandwidth. However, its implementation is very difficult due to the huge higher-order mode mismatch between multimode integrated waveguides and optical fibers (FMF). Here, we present an integrated coupler between the higher order modes of a silicon waveguide and those of a FMF. Our device is capable of terabit-per-second bandwidth based on the multiplexing of 4 spatial modes. It relies on a multi-stage silicon taper combined with a 3D polymer waveguide, which convert and shape the modes for one-to-one mode coupling to the FMF. We demonstrate an average crosstalk of -7 dB to the closest mode and capability for 1.92 Tb/sec data transmission in the L-band via mode and wavelength division multiplexing.
Fluid absorption in isolated perfused colonic crypts. (1995)

A spatial segregation of ion transport processes between crypt and surface epithelial cells is well-accepted and integrated into physiological and pathophysiological paradigms of small and large intestinal function: Absorptive processes are believed to be located in surface (and villous) cells, whereas secretory processes are believed to be present in crypt cells. Validation of this model requires direct determination of fluid movement in intestinal crypts. This study describes the adaptation of techniques from renal tubule microperfusion to hand-dissect and perfuse single, isolated crypts from rat distal colon to measure directly fluid movement. Morphologic analyses of the isolated crypt preparation revealed no extraepithelial cellular elements derived from the lamina propria, including myofibroblasts. In the basal state, crypts exhibited net fluid absorption (mean net fluid movement = 0.34 +/- 0.01 nl.mm-1.min-1), which was Na+ and partially HCO3- dependent. Addition of 1 mM dibutyryl-cyclic AMP, 60 nM vasoactive intestinal peptide, or 0.1 mM acetylcholine to the bath (serosal) solution reversibly induced net fluid secretion (net fluid movement approximately -0.35 +/- 0.01 nl.mm-1.min-1). These observations permit speculation that absorption is a constitutive transport function in crypt cells and that secretion by crypt cells is regulated by one or more neurohumoral agonists that are released in situ from lamina propria cells. The functional, intact polarized crypt described here that both absorbs and secretes will permit future studies that dissect the mechanisms that govern fluid and electrolyte movement in the colonic crypt.