gebhart/patrepeval huggingface dataset:
Cilostazol protects rat chondrocytes against nitric oxide-induced apoptosis in vitro and prevents cartilage destruction in a rat model of osteoarthritis (2008)

Objective

To examine whether cilostazol, a selective phosphodiesterase type III inhibitor, protects rat articular chondrocytes against nitric oxide (NO)–induced apoptosis and prevents cartilage destruction in mono-iodoacetate–induced osteoarthritis (OA) in a rat model in which inducible nitric oxide synthase (iNOS) is expressed.



Methods

The NO donor sodium nitroprusside was administered to rat articular chondrocytes that had been pretreated with cilostazol. Induction of apoptosis was evaluated by DNA electrophoresis and pulsed-field gel electrophoresis. The expression level and the subcellular location of apoptosis-associated factors were examined by Western blot analysis and confocal microscopy, respectively. Protein kinase CK2 (PKCK2) activity was also assayed. To examine whether orally administered cilostazol prevents cartilage destruction in vivo, cartilage samples obtained from rats with experimentally induced OA were subjected to hematoxylin and eosin, Safranin O, and TUNEL staining and immunohistochemical analysis of iNOS expression.



Results

Cilostazol prevented NO-induced reduction in viability, in a dose-dependent manner. It also prevented the up-regulation of phosphorylated p53 and p38, the down-regulation of heme oxygenase 1, the subcellular translocation of apoptosis-inducing factor and cytochrome c, and the activation of caspases 3, 7, and 8 induced by NO treatment, indicating that cilostazol prevented NO-induced cell death by blocking apoptosis. In addition, cilostazol prevented NO-induced translocation of cleaved Bid onto mitochondria, and caused phosphorylated Bid to accumulate in the nucleus and cytosol. Cilostazol prevented the down-regulation of PKCK2 and the reduction in PKCK2 activity induced by NO, indicating that its apoptosis-preventing activity was mediated via PKCK2. It also prevented chondrocyte apoptosis and cartilage destruction in a rat model of experimentally induced OA.



Conclusion

Our findings indicate that cilostazol prevents NO-induced apoptosis of chondrocytes via PKCK2 in vitro and prevents cartilage destruction in a rat model of OA.
Differential gene expression in motor and sensory Schwann cells in the rat femoral nerve (2011)

Phenotypic differences in Schwann cells (SCs) may help to guide axonal regeneration down motor or sensory specific pathways following peripheral nerve injury. The goal of this study was to identify phenotypic markers for SCs harvested from the cutaneous (sensory) and quadriceps (motor) branches of the rat femoral nerve and to study the effects of expansion culture on the expression patterns of these motor or sensory phenotypic markers. RNA was extracted from SCs harvested from the motor and sensory branches of the rat femoral nerve and analyzed using Affymetrix Gene Chips (Rat Genome 230 v2.0 Array A). Genes that were upregulated in motor SCs compared with the sensory SCs or vice versa were identified, and the results were verified for a subset of genes using quantitative real-time polymerase chain reaction (qRT-PCR). The expression levels of the "phenotype-specific" genes were then evaluated in SC expansion cultures at various time points over 30 days by qRT-PCR to determine the effect of expansion on SC phenotype. Expression levels of the phenotype-specific genes were significantly altered after expansion culture for both the motor and the sensory markers compared with fresh nerve tissue. These results indicate that both motor and sensory SC gene expression patterns are disrupted during expansion in vitro and may affect the ability of SCs to express phenotype-specific genes after transplantation.
In-Substrate Resonators and Bandpass Filters with Improved Insertion Loss in K-Band Utilizing Low Loss Glass Interposer Technology and Superlattice Conductors (2016)

In this work, we report on in-substrate passive components using a high performance glass interposer and through glass via (TGV) technology and a multi-layer superlattice conductor architecture. Minimal RF loss is achieved using low dielectric loss glass substrates and superlattice conductors featuring skin effect suppression. Half mode substrate integrated waveguide (HMSIW) resonators and two-pole bandpass filters, embedded inside a glass interposer substrate, are used as test vehicles for the demonstration of insertion loss improvement in K-band. The utilized conductor is made of 20 layers of Cu/NiFe with each pair of 360 nm/30 nm, respectively, where NiFe layers with negative permeability in frequency range of interest are used for eddy current cancelling and improving the conductor loss. Control devices using the same glass substrate and conductor made of pure copper are fabricated for comparison purposes. The glass interposer substrate (SGW3, Corning Incorporated) has a thickness of 0.13 mm and the TGV's with a diameter of 0.08 mm. Up to 0.3 dB reduction in the insertion loss is achieved by using the proposed superlattice approach on glass substrates.
Development of passive UHF RFID tag on flexible foil for sport balls pressure monitoring (2017)

In this paper, an integrated solution based on passive UHF RFID tag sensor is proposed to measure the pressure on different types of sports balls. A comparison of two commercial piezzoresistive sensors based on different technologies is performed for this type of pressure measurement application. Several external sensor interface chip configurations (SFE) have been tested and adapted to the resistance variation range that presents each type of sensor. In both function modes: passive or semi-passive, a smart RFID tag concept is fully validated through measurements on different types of sport balls and the results are compared with data from Electronic Ball Pressure Gauge CJ-01 model.
Building XML statistics for the hidden web (2003)

There is currently a lot of interest in developing Internet query processors that can pose elaborate queries on XML data on the Web.Such query processors can query data sources that have static XML files, but they should also be able to query "hidden Web" data sources that export an XML view of data stored in a database.To optimize queries that involve these hidden Web data sources, we need to have XML statistics that can be used to estimate the selectivity of queries posed to these sources.Since we can only access the data at a hidden Web data source by issuing queries, we need to develop on-line XML statistics that are built by observing queries to a hidden Web data source and their result sizes.In this paper, we assume that queries to a hidden Web data source are XPath selections from a virtual XML document that represents all the data at this source.We observe the user XPath queries to the data source and convert them to a more abstract and generalized form that we call annotated path expressions.We describe an on-line statistics structure that stores such annotated path expressions and information about their selectivity for use in estimating the selectivity of future XPath queries.We experimentally demonstrate the convergence and accuracy of our proposed on-line statistics using real and synthetic XML data sets.
Design of Mechanism and Preliminary Field Validation of Low-Cost, Passive Prosthetic Knee for Users With Transfemoral Amputation in India (2015)

An estimated 230,000 above-knee amputees in India are currently in need of prosthetic care, a majority of them facing severe socio-economic constraints. However, only few passive prosthetic knee devices in the market have been designed for facilitation of normative gait kinematics and for meeting the specific daily life needs of above-knee amputees in the developing world. Based on the results of our past studies, this paper establishes a framework for the design of a low-cost prosthetic knee device, which aims to facilitate able-bodied kinematics at a low metabolic cost. Based on an exhaustive set of functional requirements, we present a prototype mechanism design for the low-cost prosthetic knee. The mechanism is implemented using an early stance lock for stability and two friction dampers for achieving able-bodied kinematics and kinetics of walking. For early-stage validation of the prosthesis design, we carry out a preliminary field trial on four above-knee amputees in India and collect qualitative user feedback. Future iterations of the mechanism prototype will incorporate an additional spring component for enabling early stance flexion-extension.
Developmental downregulation of P2X3 receptors in motoneurons of the compact formation of the nucleus ambiguus (2005)

Motoneurons of the compact division of the nucleus ambiguus (cNA) are the final output neurons of the swallowing pattern generator. Thus, their normal function is critical to neonatal survival. To explore the role of purinergic signaling in modulating the excitability of these motoneurons during development, immunohistochemical and whole-cell recording techniques were used to characterize expression patterns of ionotropic P2X receptors and the effects of ATP on cNA motoneurons. Medullary slices containing the cNA were prepared from neonatal (P0-4) and juvenile (P15-21) rats. In neonatal cNA motoneurons, local application of 1 mM ATP produced a large (-133 +/- 17 pA; n = 78), desensitizing, inward current that was mimicked by 1 mM alpha,beta meATP and 2meSATP, and inhibited by the P2 antagonist, PPADS (5 microM), and the P2X3 antagonist, A-317481 (0.1-1 mM). In juvenile cNA motoneurons, 1 mM ATP produced negligible currents, while 10 mM ATP produced small (-59 +/- 14 pA; n = 42), primarily non-desensitizing currents. Immunohistochemistry demonstrated that in the neonate, the expression of P2X3 was robust, P2X2 and P2X5 moderate, P2X4 and P2X6 weak, and P2X1 absent. In the juvenile cNA, only low levels of P2X5 and P2X6 labeling were detected. These data indicate that P2X receptors in cNA motoneurons are profoundly downregulated during the first two postnatal weeks, and suggest a role for the purinoceptor system, particularly P2X3 receptors, in the control of esophageal motor networks during early postnatal periods.
The inflammatory status of old age can be nurtured from the intestinal environment (2008)

A better understanding of the mechanisms of host-gut microbiota cross-talk would significantly help in the design of novel nutritional strategies targeting immune reactivity at the mucosal level.
Validation of the English and Chinese versions of the Quick-FLIC quality of life questionnaire (2005)

A useful measure of quality of life should be easy and quick to complete. Recently, we reported the development and validation of a shortened Chinese version of the Functional Living Index-Cancer (FLIC), which we called the Quick-FLIC. In the present study of 327 English-speaking and 221 Chinese-speaking cancer patients, we validated the English version of the Quick-FLIC and further assessed the Chinese version. The 11 Quick-FLIC items were administered alongside the 11 remaining items of the full FLIC, but there appeared to be little context effect. Validity of the English version of the Quick-FLIC was attested by its strong correlation with two other measures of quality of life, and its ability to detect differences between patients with different performance status and treatment status (each P<0.001). Its internal consistency (alpha=0.86) and test-retest reliability (intraclass correlation=0.76) were also satisfactory. The measure was responsive to changes in performance status (P<0.001). The Chinese version showed similar characteristics. The Quick-FLIC behaved in ways that are highly comparable with the FLIC, even though the Quick-FLIC comprised only 11 items whereas the FLIC comprised 22. Further research is required to see whether the use of shorter instruments can improve data quality and response rates, but the fact that shorter instruments place less burden on the patients is itself inherently important.
Overhauser Dynamic Nuclear Polarization Enhanced Two-Dimensional Proton NMR Spectroscopy at Low Magnetic Fields (2021)

Abstract. The majority of low-field Overhauser Dynamic Nuclear Polarization (ODNP) experiments reported so far have been 1D NMR experiments to study hydration dynamics. In this work, we demonstrate the application of ODNP-enhanced 2D J-resolved (JRES) spectroscopy to improve spectral resolution beyond the limit imposed by the line broadening introduced by the paramagnetic polarizing agent. Using this approach, we are able to separate the overlapping multiplets of ethyl crotonate into a 2nd dimension and clearly identify each chemical cite individually. Crucial to these experiments is interleaved spectral referencing, a method introduced to compensate for temperature induced field drifts over the course of the NMR acquisition. This method does not require additional hardware such as a field-frequency lock, which is especially challenging when designing compact systems.
Influence of Viscoelasticity Behavior on Liquid Film Surface Appearance in Multilayered Polymer Solution (2008)

In this study, through using three-layer channels and polymer solution, it is aimed to evaluate the influence that the flow condition (flow rate, shape of channel and solution property) in the channel exerts on the liquid film surface appearance immediately after the discharge, and to clarify the rule factor in which it influences the liquid film surface appearance. Experiments were conducted by taking pictures of the bead surface appearance immediately after the discharge with a high-speed camera by using three-layer channels where the change in the merging point shape was possible. As a result of the examination, the following results were obtained.(1) As a result of the observation of surface, it is understood a peculiar surface ruggedness (thickness irregularity) is generated when the viscoelastic fluid is used in the direction of the liquid film discharge different from Melt fracture and Sharkskin. It is thought that this irregular thickness is a peculiar phenomenon to the viscoelastic fluid, because when Newtonian fluid is injected, this irregular thickness is not generated, and it is suppressed because of increased residence time in channel.(2) The factor separation between layers is done at an irregular thickness to specify the rule factor, therefore it has been understood that the correlation is strong in the first normal stress difference generated in the sub-fluid when merging for the incidence of this irregular thickness. Concretely, it is understood that the thickness irregular incidence increases as the first normal stress difference generated in the sub-fluid when merging grows. Moreover, the influence of the main-fluid on the incidence of an irregular thickness in this system is understood to be negligible.
A dynamic model for the auroral field line plasma in the presence of field-aligned current (1982)

We have developed a dynamic numerical model of the plasma along an auroral field line in order to provide a vehicle for studying ionosphere-magnetosphere coupling processes. The model is a multi-moment multi-fluid approximation of a gyrotropic plasma consisting of three species (electrons, hydrogen ions, oxygen ions) along a segment of auroral magnetic field line extending from an altitude of 800 km to 18 earth radii. We have performed preliminary simulations for the case of a current-free polar wind equilibrium of the field line plasma and the case in which a large upward field-aligned current is applied to the field line. In the former case, the agreement between our model and previous static results is reasonable given the differing boundary conditions inherent in the two cases. In the case of field-aligned current, we note that the flux tube plasma responds to the current on several time scales. After an initial rapid response due to electron-ion Joule heating, thermal oscillations of the flux tube plasma persist on time scales of the order of one hour, illustrating the complicated nature of the response of a collisionless plasma when heat flow transport is treated in a dynamic manner. (Author)
Next-generation EUV lithography productivity (Conference Presentation) (2017)

Beyond EUV insertion to high-volume manufacturing, the extendibility of the technology is dependent on the cost scaling advantages of high-NA or multi-patterning EUV lithography. Several concerns have been raised regarding the cost and lithographic feasibility of high-NA, including resist performance, productivity, depth of focus, mask infrastructure and field utilization/stitching capability. The intrinsic requirement of half-field exposures for high-NA lithography drives a necessary investigation on reduced field utilization verses stitching performance of the separate half fields. Furthermore, the additional mask for full fields dies will drive additional cost, complexity and overall overhead as compared to EUV NA 0.33 double patterning or other self-aligned technique. Here, the implication to EUV throughput capacity is analyzed within the context of the 7/5/3 nm technology nodes, specifically considering field utilization and scanner productivity as a function of source power and resist dose.
Novel Liquid Crystal Admet Polymers with Banana Main-Chain Mesogens (2005)

ABSTRACT We prepared novel macromolecular architectures of main-chain polymers poly(1)–poly(6) by a ruthenium-catalyzed acyclic diene metathesis (ADMET) polymerization of banana diene monomers 1–6. Copolymerization with a calamitic diacrylate monomer 7 yielded a copolymer poly(1-alt-7) in which the banana and the calamitic units regularly alternated along the polymer main chain. We also started the structural characterization of the polymers and found a varied mesophase behavior depending on the nature of the banana mesogen incorporated.
Repeated anti-CEA-radioimmunotherapy (RAIT) with 131iodine-labetuzumab after resection of colorectal liver metastases (CLM): Long-term results of a prospective phase II study. (2015)

719 Background: Previously, single anti-CEA-radioimmunotherapy (RAIT) with 131 iodine(I)-labetuzumab after complete (R0) resection of CEA-positive CLM was well tolerated and improved overall survival (OS) compared to a control group without RAIT. In this phase II study, we examined safety, feasibility, and long-term efficacy of repeated RAIT in the same setting. Methods: After R0-resection of CEA-positive CLM, 63 pts (42 m, 21 f; median age, 64.5 yrs) with synchronous (n=33) or metachronous (n=30) CLM received RAIT with 40-50 mCi/m 2 per dose. 45 pts were intermediate/high risk for early metastatic relapse according to the Fong score. Restaging with CT/MRI and FDG-PET was performed prior to each RAIT. Pts with persistent elevated serum CEA-levels or inconclusive lesions during post-operative restaging received RAIT, but were classified as “non-adjuvant.” Toxicity was classified according to NCI-CTC v2.0. Time to progression (TTP), OS and cancer-specific survival (CSS) were calculated. The median follow-up was 54 (range 6-127) mos. Results: After the first cycle of RAIT 14 of 63 pts experienced grade 4 hematological toxicity. Nineteen pts did not receive the second cycle of RAIT due to prolonged toxicity, impaired performance status (n=6), or metastatic relapse (n=13). The latter were further treated by resection (n=3) or systemic chemotherapy (n=10). Forty-four pts received the planned second cycle of RAIT. Of these, 4 pts newly experienced grade 4 hematological toxicity. For all 63 pts, the median TTP, OS and CSS were 13, 57 and 92 months, respectively. The truly “adjuvant” pts (n=39) had a an improved median TTP (26 vs. 6.6 mos, p&lt;0.0001), OS (76 vs. 42 mos, p=0.03) and CSS (not reached vs. 42 mos, p=0.003) in comparison to “non-adjuvant” pts (n=24). Conclusions: Repeated anti-CEA-RAIT with 131 I-labetuzumab is safe, feasible, and well-tolerated (100% compliance), with expected hematological toxicity. The long-term survival after RAIT is very encouraging, in particular for pts deemed truly “adjuvant” post-salvage resection of CLM at restaging prior to RAIT.
ChemInform Abstract: PREPARATION OF RUTHENIUM(0) AND OSMIUM(0) CYCLOOCTATETRAENE COMPLEXES (1973)

Abstract Eine THF‐Lösung des Dikaliumsalzes von Cyclooctatetraen reagiert mit dem Ru‐Komplex (Ia) zu dem fünffach‐koordinierten Derivat (IIa), in dem nach IR‐ Daten Cyclooctatetraen als dreizähniger Ligand vorliegt.
Evaluation of a new epicardial cryoablation system for the treatment of Cardiac Tachyarrhythmias (2018)

Background: With the recent reports of success, there is now increasing use of cryoablation as a standalone, minimally invasive procedure to treat various cardiac arrhythmias. With increased use, a need for new devices and treatment strategies to deliver improved outcomes has emerged. In this study we investigated the performance of a new super critical nitrogen (SCN) epicardial cryoprobe for the targeted ablation of cardiac tissue. We hypothesized that the SCN technology would enable the rapid, effective delivery of ultra-cold temperatures thereby enabling more effective ablation.
Coumestoside A, Coumestoside B and Erythrodiside A, Three Glycosides from Cylicodiscus gabunensis (Mimmosaceae) (2007)

Chemical analysis of the stem bark of Cylicodiscus gabunensis (Mimosaceae) yielded, in addition to the known eryhrodiol (4) and sojagol (IV) (5), three new glycosides designated coumestoside A (1), coumestoside B (2) and erythrodiside A (3). Their structures were established by chemical and spectroscopic means as 9- O-β-D-glucopyranosyl-4-hydroxycoumestrol, 9- O-α-L-rhamnopyranosyl-4-hydroxycoumestrol and 3- O-α-L-rhamnopyranosylerythrodiol-28- O-β-D-galactopyranoside, respectively.
1P117 Unfolding and refolding of three CutAls from P. horikoshii, T. thermophilus, and O. sativa, with unusually high denaturation temperatures(3. Protein folding and misfolding (1),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006) (2006)

Recently, we have found that the CuLAI (PhCutA]) ffom R horikosltii, a hypcrthcrmophile. has an unbelievably high denatuTtttion temperature ef nearly 1 50 OC. To elucidate the mechanism of this unusually high stability, we compared pTopenies of unfolding and refolding for thc thrce CutAls frem R horikoshii, Ztherm`rphilus (TK]utAl) and O. sativa (OsCutAb with difierent growth temperatures. PhCutAl and TtClltAl are a trimer form in sotutien and OsCutA! might bc thc same. The molecular weights of a monomeT are 11.6K to 12.SK for the three proteins. At pH 7 denamranion tempcraturcs ot' PhCutAl, 7kCutAl, ttnd OsCutAl, were found Le be 148,5, II3,9, and 98.9 eC, respectively, frorn DSC experiments, indicating that evcn OsCutAl from u plant has an unusuaUy high
Preparation of Pb(Mg1/3Nb2/3)O3–Pb(Zr,Ti)O3 Thin Films by RF-Magnetron Sputtering and Their Electrical and Piezoelectric Properties (2009)

The 0.875Pb(Mg1/3Nb2/3)O3–0.125Pb(Zr,Ti)O3 [0.125PMN–0.875PZT (50/50)] thin films, which have an extremely large piezoelectric coefficient d31 of -225 pm/V, have been successfully obtained on (100) Si substrates by RF-magnetron sputtering. These PMN–PZT thin films have a high (001) orientation. The dependences of the electrical and piezoelectric properties of these films on substrate heating temperature were evaluated. The εr of PMN–PZT films prepared in the substrate heating temperature range of 540 to 620 °C was almost constant at 1400. The d31 was measured from the tip deflection of the cantilever beams. Even without a poling treatment, the d31of the PMN–PZT thin films at 580 °C was -225 pm/V. This d31 was 50% larger than that of PZT thin films deposited under the same conditions. These PMN–PZT thin films are attractive materials for micro actuator applications, especially for ink jet head actuators.
Antibodies and Brain Disease: A Convergence of Immunology and Physiology (2006)

The authors discuss a new study, by Emmer and colleagues, which attempted to test whether the presence of an antibody called anti-NMDAR correlates with brain abnormality in patients with SLE.
Clinical Outcomes and Response to Vericiguat According to Index Heart Failure Event (2020)

ClinicalTrials.gov Identifier: NCT02861534.