gebhart/patrepeval huggingface dataset:
Conceptus interferon gamma is essential for establishment of pregnancy in the pig (2021)

Establishment and maintenance of pregnancy in the pig is a complex process that relies on conceptus regulation of the maternal proinflammatory response to endometrial attachment. Following elongation, pig conceptuses secrete interferon gamma (IFNG) during attachment to the endometrial luminal epithelium. The objective here was to determine if conceptus production of IFNG is important for early development and establishment of pregnancy. CRISPR/Cas9 gene editing and somatic cell nuclear transfer technologies were used to create an IFNG loss-of-function study in pigs. Wild-type (IFNG+/+) and null (IFNG-/-) fibroblast cells were used to create embryos through somatic cell nuclear transfer. IFNG expression was not detected in IFNG-/- conceptuses on either day 15 or day 17 of pregnancy. Ablation of conceptus IFNG production resulted in the reduction of stromal CD3+ and mast cells, which localized to the site of conceptus attachment on day 15. The uteri of recipients with IFNG-/- conceptuses were inflamed, hyperemic and there was an abundance of erythrocytes in the uterine lumen associated with the degenerating conceptuses. The endometrial stromal extracellular matrix was altered in the IFNG-/- embryo pregnancies and there was an increased endometrial mRNA levels for collagen XVII (COL17A1), matrilin 1 (MATN1), secreted phosphoprotein 1 (SPP1), and cysteine-rich secretory protein 3 (CRISP3), which are involved with repair and remodeling of the extracellular matrix. These results indicate conceptus IFNG production is essential in modulating the endometrial proinflammatory response for conceptus attachment and survival in pigs.
A 2-step approach to myeloablative haploidentical stem cell transplantation: a phase 1/2 trial performed with optimized T-cell dosing (2011)

Studies of haploidentical hematopoietic stem cell transplantation (HSCT) have identified threshold doses of T cells below which severe GVHD is usually absent. However, little is known regarding optimal T-cell dosing as it relates to engraftment, immune reconstitution, and relapse. To begin to address this question, we developed a 2-step myeloablative approach to haploidentical HSCT in which 27 patients conditioned with total body irradiation (TBI) were given a fixed dose of donor T cells (HSCT step 1), followed by cyclophosphamide (CY) for T-cell tolerization. A CD34-selected HSC product (HSCT step 2) was infused after CY. A dose of 2 × 10(8)/kg of T cells resulted in consistent engraftment, immune reconstitution, and acceptable rates of GVHD. Cumulative incidences of grade III-IV GVHD, nonrelapse mortality (NRM), and relapse-related mortality were 7.4%, 22.2%, and 29.6%, respectively. With a follow-up of 28-56 months, the 3-year probability of overall survival for the whole cohort is 48% and 75% in patients without disease at HSCT. In the context of CY tolerization, a high, fixed dose of haploidentical T cells was associated with encouraging outcomes, especially in good-risk patients, and can serve as the basis for further exploration and optimization of this 2-step approach. This study is registered at www.clinicaltrials.gov as NCT00429143.
Robust Burg estimation of stationary autoregressive mixtures covariance (2015)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation Alexis Decurninge, Frédéric Barbaresco; Robust Burg estimation of stationary autoregressive mixtures covariance. AIP Conference Proceedings 13 January 2015; 1641 (1): 387–394. https://doi.org/10.1063/1.4906002 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAIP Publishing PortfolioAIP Conference Proceedings Search Advanced Search |Citation Search
<title>Disordering of InGaAs/GaAs strained quantum well structures induced by rare gas ion implantation</title> (1994)

In this work we have investigated the effect of various implantation schemes on In(0.2)GaAs/GaAs/AlGaAs Single Quantum Well, where the implanted species are Argon and Helium, with doses in the range 1E12 to 1E14 at cm^2, at energy spanning 270 - 400 KeV and 30 to 50 KeV for Ar and He, respectively. Repetitive annealing processes were carried out between 735 and 870 degree(s)C and the interdiffusion was deduced by photoluminescence measurements. A maximum of 20 nm shift from He ion implanted Quantum Well with an high degree of reconstruction has been recorded, thus allowing the application of this disordering scheme for the realization of optoelectronic devices.
Poster: Characterizing driving behaviors through a car simulation platform (2017)

Human mobility has opened up to many themes in recent years. Human behavior and how a driver might react to certain situations, whether dangerous (e.g. an accident) or simply part of the evolution of new technologies (e.g. autonomous driving), leaves many avenues to be explored. Although experiments have been deployed in real situations, it remains difficult to encounter the conditions that certain studies may require. For this reason, we have set up a driving simulator (comprising several modules) that is able to reproduce a realistic driving environment. Although, as the literature has already demonstrated, the conditions are often far from reality, simulation platforms are nonetheless capable of reproducing an incredibly large number of scenarios on the fly (e.g. [1], [2]). In this poster, we explain how we conceived the simulator, its architecture, as well as the system we developed for collecting metrics on both the driver and the simulation environment. In addition, we take advantage of this conference to publicly share a dataset consisting of 25 drivers performing the same road circuit on the “Project Cars” <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sup> game.
A smart camera for face recognition (2005)

There is a rapidly growing demand for using smart cameras for various biometric applications in surveillance. Although having a small form-factor, most of these applications demand huge processing performance for real-time processing. Face recognition is one of those applications. In this paper we show that we can run face recognition in real-time by implementing the algorithm on an architecture which combines a parallel pixel processor: with a digital signal processor. The algorithm consists of a cascade of filters for detection, registration and normalization and an RBF neural network with temporal filtering. Everything fits within a digital camera, the size of a normal surveillance camera.
Two-dimensional apodized grating coupler for polarization-independent and surface-normal optical coupling (2020)

A four-port grating coupler with etched square holes is proposed and demonstrated for surface-normal and polarization independent coupling. Benefiting from the perfectly vertical coupling and 1 × 4 power splitting/combing scheme, efficient polarization independent operation was achieved. The coupling efficiency was further improved by grating apodization. According to the simulations, the proposed 2D apodized grating coupler can achieve a coupling efficiency (CE) of 64.5% (-1.9 dB), a low upward back-reflection of 8.5% (-11 dB), and polarization-dependent loss (PDL) lower than 0.04 dB across the wavelength range of 1520-1620 nm. A CE of 56.3% (-2.5 dB) was experimentally measured, with PDL below 0.3 dB within the C-band and lower than 0.5 dB within the L-band. Waveguide delay lines were used to compensate the waveguide channel phase differences at the output port.
Improving Early Identification of Significant Weight Loss Using Clinical Decision Support System in Lung Cancer Radiation Therapy (2021)

Machine learning-based CDSS showed the potential to improve SWL prediction in lung cancer RT. More investigation on a larger patient cohort is needed to properly interpret CDSS prediction performance and its benefit in clinical decision making.
Incidence, mitigation, and management of neurologic adverse events in patients with multiple myeloma (MM) treated with ciltacabtagene autoleucel (cilta-cel) in CARTITUDE-2. (2021)

8028 Background: Cilta-cel (JNJ-68284528) is a chimeric antigen receptor T (CAR-T)-cell therapy with 2 BCMA-targeting, single-domain antibodies designed to confer high avidity binding. CARTITUDE-2 (NCT04133636) is a phase 2, multicohort, open-label study assessing the efficacy and safety of cilta-cel in patients (pts) with MM in various clinical settings. Here, we describe the mitigation and management strategies implemented to identify and reduce the risk for neurologic adverse events (AEs) in Cohort A pts (progressive MM after 1−3 prior lines of therapy). Methods: Eligible pts (≥18 years of age) had MM per IMWG criteria, measurable disease, ECOG ≤1, progressive disease after 1−3 prior lines of therapy (including a PI and IMiD) and were lenalidomide refractory (no prior BCMA-targeting agent). Cilta-cel (0.75×10 6 [range 0.5–1.0×10 6 ] CAR+ viable T cells/kg) was given as a single infusion 5–7 days after start of lymphodepletion (cyclophosphamide 300 mg/m 2 + fludarabine 30 mg/m 2 daily for 3 days). Monitoring and mitigation strategies for neurologic AEs include providing more effective bridging therapy to reduce tumor burden prior to lymphodepletion, frequent assessment of CAR-T-related ICANS using the ICE tool, regular handwriting assessments to detect micrographia, and neuroimaging (brain MRI) and EEG for pts with prior neurologic disease. Management strategies include evaluating infectious and paraneoplastic etiologies upon observation of ICANS ≥Grade (gr) 1, administration of tocilizumab (if concurrent CRS, all gr of ICANS) and/or dexamethasone (gr 2/3) or methylprednisolone (gr 4). ICANS and CRS were graded by ASTCT criteria; neurotoxicities not classified as ICANS were graded per CTCAE Version 5.0. Results: As of 15 Jan 2021 (median follow-up: 5.8 months [range: 2.5–9.8 months]), 20 pts in Cohort A received cilta-cel. Median age was 60 years (range: 38–75); 65% were male. Neurotoxicities occurred in 4 pts (20%). Three pts had ICANS (gr 1/2); median time to onset of symptoms was 8 days (range: 7–11) and median duration was 2 days (range: 1–2). Two of the 3 pts received supportive measures to treat ICANS, including levetiracetam and steroids; all 3 had concurrent CRS and all recovered. One pt developed isolated facial paralysis (gr 2) on Day 29 after cilta-cel infusion, and recovered 51 days after the onset of event following treatment with dexamethasone for 28 days. No movement or neurocognitive disorders were reported. Conclusions: Neurologic AEs were generally manageable in pts with MM following treatment with cilta-cel. With a median of 5.8 months of follow-up, there were no movement or neurocognitive disorders in pts from Cohort A. These results suggest that early detection and management of neurologic AEs can lead to better treatment outcomes. Clinical trial information: NCT04133636.
Extended Effects of a Wearable Sensory Prosthesis on Gait, Balance Function and Falls After 26 Weeks of Use in Persons with Peripheral Neuropathy and High Fall Risk – The walk2Wellness Trial (2022)

Abstract Background We recently reported that individuals with impaired plantar sensation and high fall risk due to sensory peripheral neuropathy (PN) improved gait and balance function following 10 weeks of use of Walkasins ® , a wearable lower limb sensory prosthesis that provides directional tactile stimuli related to plantar pressure measurements during standing and walking (RxFunction Inc., MN, USA). Here, we report 26-week outcomes and compare pre- and in-study fall rates. We expected improvements in outcomes and reduced fall rates reported after 10 weeks of use to be sustained. Methods Participants had clinically diagnosed PN with impaired plantar sensation, high fall risk (Functional Gait Assessment, FGA score &lt;23) and ability to sense tactile stimuli above the ankle at the location of the device. Additional outcomes included 10m Gait Speed, Timed Up&amp;Go (TUG), Four-Stage Balance Test, and self-reported outcomes, including Activities-Specific Balance Confidence scale and Vestibular Disorders Activities of Daily Living Scale. Participants tracked falls using a calendar. Results We assessed falls and self-reported outcomes from 44 individuals after 26 weeks of device use; 30 of them conducted in-person testing of clinical outcomes. Overall, improvements in clinical outcomes seen at 10 weeks of use remained sustained at 26 weeks with statistically significant increases compared to baseline seen in FGA scores (from 15.0 to 19.2), self-selected gait speed (from 0.89 m/s to 0.97 m/s), and 4-Stage Balance Test (from 25.6s to 28.4s), indicating a decrease in fall risk. Non-significant improvements were observed in TUG and fast gait speed. Overall, 39 falls were reported; 31 of them did not require medical treatment and four caused severe injury. Participants who reported falls over 6 months prior to the study had a 43% decrease in fall rate during the study as compared to self-report 6-month pre-study (11.8 vs. 6.7 falls/1000 patient days, respectively, p&lt;0.004), similar to the 46% decrease reported after 10 weeks of use. Conclusion A wearable sensory prosthesis can improve outcomes of gait and balance function and substantially decreases incidence of falls during long-term use. The sustained long-term benefits in clinical outcomes reported here lessen the likelihood that improvements are placebo effects. Trial registration ClinicalTrials.gov (# NCT03538756 )
Purification of large peptides using Chemoselective tags (1999)

In solid phase peptide synthesis (SPPS), deletion sequences are generated at each addition of amino acid due to non-quantitative coupling reactions. Their concentration increases exponentially with the length of the peptide chain, and after many cycles not only do they represent a large proportion of the crude preparation, but they can also exhibit physicochemical characteristics similar to the target sequence. Thus, these deletion-sequence contaminants present major problems for removal, or even detection. In general, purification of synthetic peptides by conventional chromatography is based on hydrophobicity differences (using RP-HPLC) and charge differences (using ion-exchange chromatography). For short sequences, the use of one or both techniques is in general sufficient to obtain a product with high purity. However, on increasing the number of amino acid residues, the peptide secondary and progressively tertiary and quaternary structures begin to play an important role and the conformation of the largest peptides can decisively affect their retention behaviour. Furthermore, very closely related impurities such as deletion sequences lacking one or few residues can be chromatographically indistinguishable from the target sequence. Therefore, purification of large synthetic peptides is a complex and time-consuming task that requires the use of several separation techniques with the inevitable dramatic reduction in yields of the final material. Permanent termination (capping) of unreacted chains using a large excess of an acylating agent after each coupling step prevents the formation of deletion sequences and generates N-truncated peptides. However, even under these more favourable conditions, separation of the target sequence from chromatographically similar N-capped polypeptides requires extensive purification. If the target sequence could be specifically and transiently labelled so that the resulting product were selectively recognized by a specific stationary phase, then separation from impurities should be facilitated. This chapter deals with such an approach and in particular with the purification of large polypeptides, assembled by solid phase strategy, using lipophilic and biotin-based 9-fluorenylmethoxycarbonyl (Fmoc) chromatographic probes. Assuming that the formation of deletion sequences is prevented by capping unreacted chains, a reciprocal strategy can be applied that involves functional protection of all polymer-supported peptide chains that are still growing, with a specially chosen affinity reagent or chromatographic probe.
An avian model for studies of embryonic lymphangiogenesis. (2000)

Embryonic development of lymphatics (lymphangiogenesis) in recent years has rarely been studied experimentally. Using an avian model, we showed that both intra- and extra-embryonic blood vessels of chick and quail embryos are accompanied by lymphatics. The lymphatics of the chorioallantoic membrane (CAM) are drained by lymphatic trunks of the umbilicus and are connected to the posterior lymph hearts. Intra-embryonic lymphatics are drained via paired thoracic ducts into the jugulo-subclavian junction. The lymphatic endothelial cells are characterized by the expression of Vascular Endothelial Growth Factor Receptors (VEGFR) -2 and -3. Application of VEGF-C, the ligand of these two receptors, on the differentiated CAM, induces proliferation of lymphatic endothelial cells and formation of huge lymphatic sinuses. These lymphatics derive from pre-existing lymphatic endothelial cells, whereas, in early embryos lymphangioblasts are present in the mesenchyme. This phenomenon can be demonstrated by interspecific grafting experiments between chick and quail embryos. Together with the early lymph sacs, the lymphangioblasts form the embryonic lymphatic system. Our studies demonstrate the importance of lymphangioblasts and lymphangiogenic growth factors in embryonic lymphangiogenesis.
Fluidity dependence of deprotonation kinetics of chemically amplified resist (2014)

Chemically amplified resists have been widely used in the mass production line. An acid generation mechanism induced by ionizing radiation with extreme ultraviolet (EUV) and electron beam is an important issue for improvement of the resist performance such as sensitivity, roughness, and resolution below 16 nm. However, the details of deprotonation kinetics from the ionized resist solid film immediately after the ionization have been still unclear. In this study, pulse radiolysis of highly concentrated poly(4-hydroxystylene) (PHS) solutions was performed. The viscosity dependence on the deprotonation dynamics of the ionized concentrated solutions was investigated to clarify the proton generation of ionized PHS in a medium with low mobility. The deprotonation from the PHS radical cation becomes slower with increasing PHS concentration. It is suggested that the deprotonation reaction is slower in a less mobile medium because of decrease of the molecular motions.
Study on Calcium Oxalate Monohydrate Renal Uroliths III. Composition and Density (1995)

Density and content of mineral constituents were determined for 33 human calcium oxalate monohydrate (hereafter COM) uroliths whose external appearance and inner structure were described in part I and II respectively. Studied stones contained 0.13-0.42 wt.% of struvite, 0.68-4.12 wt.% of hydroxyapatite, 73-96 wt.% of COM and 3-10 wt.% of water unbound in a crystallohydrate 10 to 20 wt.% of calculus mass is not accounted for by chemical analysis. Density of COM calculi varying between 1.67 and 2.06 g cm-3 is not a function of any single stone parameter. Around 30% of stone volume is not occupied by crystalline components. The mulberry stones of sedimentary origin contained higher amount of organic matter than papillar and mulberry stones displaying site of attachment to epithelium.
TheCampylobacter jejuniglycome (2005)

Microbial cell surface glycans in the form of glycolipids and glycoproteins frequently play important roles in cell–cell interaction and host immune responses. Given the likely importance of these surface structures in the survival and pathogenesis of Campylobacter jejuni, a concerted effort has been made to characterise these determinants genetically and structurally since the genome was sequenced in 2000. We review the considerable progress made in characterising the Campylobacter glycome including the lipooligosaccharide (LOS), the capsule and O- and N-linked protein glycosylation systems, and speculate on the roles played by glycan surface structures in the life-cycle of C. jejuni.
Effects of Transport and Storage Conditions on Gene Expression in Blood Samples (2016)

The use of dedicated biomarkers or time course experiments should be performed in order to verify potential bias on gene expression analysis due to sample shipment and storage conditions. Alternatively, the use of RNA stabilizer containing tubes can represent a reliable option to avoid ex vivo RNA changes.
MOCVD of advanced dielectric and ferroelectric films by liquid delivery (1997)

Abstract An MOCVD technique has been developed for (Ba,Sr)TiO3 (BST) films based on flash vaporization of metalorganic precursors with exceptional composition control and no process drift for the equivalent of ∼1300 wafers of 30 nm thickness. Charge storage density of > 60 fF/μm2 has been routinely obtained for 30 nm films and values up to 100 fF/μm2 have been observed for films with electrical leakage < 10−7 A/cm2. The method has also been applied to other materials systems, including Pb(Zr,Ti)O3 (PZT). High quality, single phase PZT films have been deposited and the effect of Zr/Ti ratio has been examined. Remanent polarization (Pr) and coercive field follow expected trends. Pr of ∼ 20 μC/cm2 was observed with fully saturated hysteresis at 3V for films with 140–175 nm thickness. Coercive voltages < 1 V have also been obtained. This finding demonstrates promise of this deposition method for low voltage applications. Key Words: Ferroelectric filmsMOCVDBSTPZT
Three‐dimensional reconstructions of human embryos: Morphogenesis and hepatic vascularization. (2013)

OBJECTIVES The purpose of this study was to describe the morphogenesis of hepatic gland and its vascularization in 4 human embryos using three‐dimensional reconstructions from histological sections, and to compare our results with literature. MATERIALS AND METHODS We investigated four embryos belonging to three prestigious historical French collections of human embryos serially sectioned: 7 mm (Carnegie Stage 14), 15 mm (CS 18), 22.5 mm (CS 21) and 27 mm (CS 23) respectively. Staining included either Masson's trichrome or standard HES. Scanning was performed using an Epson V750 PRO ®, (resolution 3200 dpi). Registration of image stacks and export in DICOM files were performed using original software developed by IADI Laboratory (INSERM U947, NANCY). The images obtained were exploited with ORS ® Visual. RESULTS From the 7mm embryo to 27mm, the hepatic gland was very large and filled almost the entire abdominal cavity. The hepatic vascularization was well visualized and individualized. The portal vein and hepatic veins were distinct from the 15mm embryo to subsequent stages. CONCLUSION This method of three‐dimensional reconstructions of hepatic morphogenesis brings a new perspective to the study of human embryology. Grant Funding Source : AAA
EFFECTS OF HYDROSTATIC PRESSURE AND OF JAHN-TELLER DISTORTIONS ON THE MAGNETIC PROPERTIES OF RbFeF3 (1971)

RbFeF3 exhibits first-order transitions at T1 = 40 °K and T2 = 87 °K. We report (ƊTi/ƊHa)p= 0.35 and 0.19 °/kOe, (ƊTi/ƊP)H = 0.18 and - 0.81°/kbar for T1 and T2, respectively, corresponding to latent heats 0.006 and 0.04 cal/g and relative volume changes ƊVi/ Vi = 1.5 x 10-6 and - 22 x 10-6. The inhomogeneity of the Néel temperature, the crystallographic structures, the weak ferromagnetism below T2, and the cubic magnetic anisotropy in an Ha > 0.5 kOe are interpreted.