gebhart/patrepeval huggingface dataset:
Two-stage optical parametric generator with multi-Watt outputs in ultrashort pulses around 800 nm and 1450 nm (2004)

With a passively mode-locked thin disk Yb:YAG laser we pumped a two-stage optical parametric generator producing ultrashort pulses with 11 W average power at 799 nm and 6 W at 1448 nm. Sum frequency mixing of the latter two wavelengths with residual 1030-nm light results in multi-watt output powers at 450 nm and 602 nm.
Familial t(1;11) translocation is associated with disruption of white matter structural integrity and oligodendrocyte-myelin dysfunction (2019)

ABSTRACT Although the underlying neurobiology of major mental illness (MMI) remains unknown, emerging evidence implicates a role for oligodendrocyte-myelin abnormalities. Here, we took advantage of a large family carrying a balanced t(1;11) translocation, which substantially increases risk of MMI, to undertake both diffusion tensor imaging (DTI) and cellular studies to evaluate the consequences of the t(1;11) translocation on white matter structural integrity and oligodendrocyte-myelin biology. This translocation disrupts among others the DISC1 gene which plays a crucial role in brain development. We show that translocation-carrying patients display significant disruption in white matter integrity compared to familial controls. At a cellular level, we observe dysregulation of key pathways controlling oligodendrocyte development and morphogenesis in induced pluripotent stem cell (iPSC) case derived oligodendrocytes. This is associated with reduced proliferation and a stunted morphology in vitro . Further, myelin internodes in a humanized mouse model that recapitulates the human translocation as well as after transplantation of t(1;11) oligodendrocyte progenitors were significantly reduced compared to controls. Thus we provide evidence that the t(1;11) translocation has biological effects at both the systems and cellular level that together suggest oligodendrocyte-myelin dysfunction.
P17-09. Immunization with a single HIV-1 envelope sequence can generate CD8+ T lymphocytes capable of recognizing multiple variant forms of envelope (2009)

Background: The ability of CD8+ T lymphocytes to recognize a diversity of mutant forms of an HIV epitope is of central importance in the immune containment of this virus. The present studies were pursued to determine the mechanism employed by CD8+ T lymphocytes to recognize mutant viruses. In particular, we sought to determine whether mutant sequences are recognized by distinct CD8+ T lymphocyte populations or whether individual clonal populations of CD8+ T lymphocytes recognize a diversity of mutant sequences. Methods: We employed flow cytometry, Vβ repertoire analysis, and CDR3 sequencing methodologies to characterize the clonal diversity of CD8+ T lymphocytes that recognize variant forms of the HIV-1 envelope (Env) p41A epitope generated after infection by SHIV-89.6P or elicited by HIV-1 89.6P Env immunization of Mamu-A*01+ rhesus monkeys. To evaluate the capacity of the CD8+ T lymphocytes to recognize genetically diverse isolates of HIV-1, we employed a series of tetramers constructed with variants of the p41A epitope of HIV-1 Env. To define which T cell receptor mediated the recognition of each specific variant p41A, we isolated variant p41A-specific CD8+ T lymphocyte populations and analyzed the expression of 46 Vβ families and subfamilies genes. We then determined the precise clones employed for the recognition of each variant epitope peptide through CDR3 sequencing. Results: In both the infected and the vaccinated monkeys, we observed clonotypes capable of recognizing the majority of the variant epitope peptides. Conclusion: These data show that exposure to a single HIV-1 Env sequence can generate clonotypes capable of recognizing multiple variant forms of HIV-1 Env. Such Env-specific CD8+ T lymphocytes should be able to confer potent, effective protection against a diverse spectrum of circulating viruses.
Continuous synthesis of zinc oxide nanoparticles in a microfluidic system for photovoltaic application (2014)

This study describes the synthesis of zinc oxide nanoparticles (ZnO NPs) using a microfluidic system. A continuous and efficient synthetic process was developed based on a microfluidic reactor in which was implemented a time pulsed mixing method that had been optimized using numerical simulations and experimental methods. Numerical simulations revealed that efficient mixing conditions could be obtained over the frequency range 5-15 Hz. This system used ethanol solutions containing 30 mM sodium hydroxide (NaOH) or 10 mM dehydrated zinc acetate (Zn(OAc)2) under 5 Hz pulsed conditions, which provided the optimal mixing performance conditions. The ZnO NPs prepared using the microfluidic synthetic system or batch-processed system were validated by several analytical methods, including transmission electron microscopy (TEM), energy dispersive X-ray spectrometry (EDS), X-ray diffraction (XRD), UV/VIS NIR and zeta (ζ) potential analysis. Bulk-heterojunction organic photovoltaic cells were fabricated with the synthesized ZnO NPs to investigate the practicability and compared with batch-process synthesized ZnO NPs. The results showed that microfluidic synthesized ZnO NPs had good preservability and stability in working solution and the synthetic microfluidic system provided a low-cost, environmentally friendly approach to the continuous production of ZnO NPs.
Synthesis and Applications of Fluorocyclopropanes (2016)

The combination of a fluorine atom and a cyclopropane ring, which both possess unique structural and chemical features, can generate new relevant scaffolds with potential interest for the synthesis of new bioactive compounds. In this review, we report the impressive progress recently devoted to the synthesis of fluorocyclopropanes especially using asymmetric methods and highlight some recent reported applications. 1 Introduction 2 Addition of Carbenes to Fluoroalkenes 3 Addition of Fluorocarbenes to Alkenes 4 Michael Initiated Ring Closure 5 Nucleophilic Fluorination 6 Applications to the Synthesis of Biorelevant Compounds 7 Conclusion
T 1 /T 2 Ratio Imaging Improves Cortical Lesion Contrast in Multiple Sclerosis on 3T MRI (2022)

ABSTRACT Background Cortical demyelinated lesions are prevalent in multiple sclerosis (MS) and associated with disability; their presence on MRI has recently been incorporated into MS diagnostic criteria. Presently, advanced and ultra-high-field MRI — not routinely available in clinical practice — are the most sensitive methods for detection of cortical lesions, and approaches utilizing MRI sequences obtainable in routine clinical practice remain an unmet need. Objective To assess the sensitivity of the ratio of T 1 -weighted and T 2 -weighted (T 1 /T 2 ) signal intensity for focal cortical lesions in comparison to other established, sensitive, advanced and high-field imaging methods. Methods 3-tesla (3T) and 7-tesla (7T) MRI collected from 10 adults with MS participating in a natural history study at the National Institutes of Health were included in the study. T 1 /T 2 images were calculated by dividing 3T T 1 w images by 3T T 2 w fluid-attenuated inversion recovery (FLAIR) images for each participant. Cortical lesions were identified using 7T T 2 *w and T 1 w images and corresponding voxels were assessed on registered 3T images. For each participant, ratios derived from the median signal intensity of nonlesional tissue in the cortical region of the lesion and the median lesional voxel intensity were computed. These values were compared across 3T imaging sequences, including the calculated T 1 /T 2 image, as well as T 1 w, T 2 w, and Inversion Recovery Susceptibility Weighted Imaging with Enhanced T 2 weighting (IR-SWIET) images. Results 614 cortical lesions were identified on 7T images. 3T T 1 /T 2 images demonstrated a larger contrast between median nonlesional cortical signal intensity and median cortical lesion signal intensity (median ratio = 1.29, range 1.19 – 1.38) when compared to T 1 w (1.01, 0.97 – 1.10, p&lt;0.002), T 2 w (1.17, 1.07 – 1.26, p&lt;0.002), and IR-SWIET (1.21, 1.01 – 1.29, p&lt;0.03). Conclusion T 1 /T 2 images are sensitive to cortical lesions. Approaches incorporating T 1 /T 2 could improve the accessibility of cortical lesion detection in research settings and clinical practice.
Stopping Criteria for Non-Negative Matrix Factorization Based Supervised and Semi-Supervised Source Separation (2014)

Numerous audio signal processing and analysis techniques using non-negative matrix factorization (NMF) have been developed in the past decade, particularly for the task of source separation. NMF-based algorithms iteratively optimize a cost function. However, the correlation between cost functions and application-dependent performance metrics is less known. Furthermore, to the best of our knowledge, no formal heuristic to compute a stopping criterion tailored to a given application exists in the literature. In this paper, we examine this problem for the case of supervised and semi-supervised NMF-based source separation and show that iterating these algorithms to convergence is not optimal for this application. We propose several heuristic stopping criteria that we empirically found to be well correlated with source separation performance. Moreover, our results suggest that simply integrating the learning of an appropriate stopping criterion in a sweep for model size selection could lead to substantial performance improvements with minimal additional effort.
Virtual reality for palmtop computers (1993)

article Free Access Share on Virtual reality for palmtop computers Authors: George W. Fitzmaurice View Profile , Shumin Zhai View Profile , Mark H. Chignell View Profile Authors Info & Claims ACM Transactions on Information SystemsVolume 11Issue 3July 1993 pp 197–218https://doi.org/10.1145/159161.159160Published:01 July 1993Publication History 100citation1,643DownloadsMetricsTotal Citations100Total Downloads1,643Last 12 Months79Last 6 weeks15 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my Alerts New Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Abstract 17219: Non-Invasive, Blood-Free Point of Care Test to Rapidly Exclude Myocardial Injury: The AI ECG (2020)

Background: Myocardial injury results in release of cardiac troponin (cTn) into the bloodstream, readily detected by high-sensitivity cTn (hs-cTn) assays. A noninvasive, rapid, home-based test to exclude acute myocardial injury at the time of the test and for the next 7 hours would help manage patients with suspected myocardial infarction. Since myocyte injury is associated with ECG changes, we hypothesized an artificial intelligence ECG (AI-ECG) could identify absence of injury. Objective: To train and test an AI-ECG convolutional neural network (CNN) to identify patients using a single ECG who are suspected of myocardial infarction who are low risk, with hs-cTn levels below the 99 th percentile at test time and for the subsequent 7 hours. Methods: A CNN tuned to identify the absence of a hs-cTnT (5 th Gen cTnT Roche Diagnostics) &gt;15ng/L for men and &gt;10ng/L for women was developed. All ECGs were recorded within one hour of the hs-cTnT assay. We used 73,012 ECGs and hs-cTnT pairs from 47,542 unique patients to train the network, 9031 ECGs from 5,811 patients for internal validation to optimize hyperparameters, and 11,904 ECGs with 21,191 hs-cTnT measurements up to 7 hours after the ECG, from 11,904 different patients as a holdout test set. Results: The mean age was 63.9±17.5 years, and 30,348 of the 59,446 patients (51%) were male. 5,852 patients (49.1%) had no elevation of hs-cTnT and 6,052 (50.9%) had an hs-cTnT above the 99 th percentile at baseline or within 7 hours of the test. Of the 11,904 patients in the test set, using a sensitive threshold, the 12 lead AI ECG identified 1037 patients (8.7%) likely to have a low risk for subsequent hs-cTnT increases &gt; 99 th % (AUC 0.86), and the single lead ECG identified 685 patients. Of the 1037 low risk pts, 59 had an hs-cTnT &gt; 99 th percentile within 7 hours. The mean maximum hs-cTnT among the 59 low-risk patients was 53 ng/L ± 92 vs 184 ng/L ± 1474 in the others. None of these low risk patients died within 14 days of the test. Conclusion: Use of the AI ECG to identify patients without cardiac injury at the time of the test and for 7 subsequent hours is feasible. This may permit rapid, point of care, home-based, bloodless, painless testing to exclude cardiac injury, facilitating care and management of patients with suspected ischemia.
Amonabactin: Characterization of a Series of Siderophores from Aeromonas hydrophila (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAmonabactin: Characterization of a Series of Siderophores from Aeromonas hydrophilaJason R. Telford, Julie A. Leary, Linda M. G. Tunstad, B. Rowe Byers, and Kenneth N. RaymondCite this: J. Am. Chem. Soc. 1994, 116, 10, 4499–4500Publication Date (Print):May 1, 1994Publication History Published online1 May 2002Published inissue 1 May 1994https://pubs.acs.org/doi/10.1021/ja00089a058https://doi.org/10.1021/ja00089a058research-articleACS PublicationsRequest reuse permissionsArticle Views223Altmetric-Citations25LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
In Vivo Tracking of Dendritic Cells in Patients With Multiple Myeloma (2008)

Dendritic cell (DC) immunotherapy is being actively studied in multiple myeloma (MM). We aimed to use positron emission tomography or single positron emission tomography to determine the in vivo distribution of monocyte-derived nonmatured DC or matured DC (mDC) administered to patients with MM. Eligible patients had stable or slowly progressive MM and elevated serum MUC-1 or MUC-1 expression on marrow plasma cells. DCs were derived from granulocyte-macrophage colony-stimulating factor+ interleukin-13 stimulated autologous monocytes, pulsed with mannan-MUC1 fusion protein, and matured by FMKp and interferon-gamma. Before injection, DCs were labeled with either 18fluorine-fluorodeoxyglucose, 111indium-oxine or 64copper-pyruvaldehyde-bis-N-4-methylthiosemicarbazone. Labeled DCs were given either as a single intravenous dose or by concurrent subcutaneous (SC), intradermal (ID), and intranodal routes. 18Fluorine-fluorodeoxyglucose tracking was unsuccessful owing to high radiolabel efflux. 64Copper-pyruvaldehyde-bis-N-4-methylthiosemicarbazone-labeled mDC (n=2 patients) demonstrated tracking to regional nodes but quantitation was also limited owing to cellular efflux. 111Indium-oxine, however, gave reproducible tracking of both nmDc and mDC (n=6) to regional lymph node after either SC or ID administration, with mDC revealing superior migration to regional lymph node. SC and ID routes produced similar levels of DC migration.
Alpha 1-adrenoceptor and purinoceptor agonists modulate Na-H antiport in single cardiac cells (1993)

We investigated the effects of an alpha 1-adrenoceptor (phenylephrine) and a purinoceptor agonist (ATP), both of which accelerate the phosphoinositide turnover, on the Na-H antiport activity of rat single cardiac cells using the pH-sensitive fluorescent indicator seminaphthorhodafluor-1 (SNARF-1). Both phenylephrine, in the presence of a beta-adrenoceptor blocker, and ATP enhanced the ability of the cell to regulate its intracellular pH (pHi) after an imposed acid load. This effect was observed in HCO3-free N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) and prevented by Na-H antiport inhibitors ethylisopropylamiloride (EIPA) or amiloride. Similar results were obtained when cells were bathed in an acidic extracellular medium. Hence, the alpha 1-adrenoceptor and purinoceptor agonists activate the Na-H antiport even when it is partially inhibited by extracellular protons. To further evaluate the effects of the two neurohormones, the rate of proton efflux was estimated as a function of the magnitude of the imposed acid load. The results indicate that the agonist-induced modulation of the Na-H antiport is caused by an acceleration of its exchange activity and by a shift of its dependence on pHi toward more alkaline pH values. The agonist-mediated stimulation of the antiport was also observed in partially depolarized cells and was not dependent on intracellular Ca. Phorbol 12-myristate 13-acetate was not able to reproduce the effects of the agonists on the Na-H antiport. Conversely, the inhibitors of protein kinase C did not prevent the activation of the antiport by the neurohormones. Thus our data suggest that neither a Ca-calmodulin-dependent kinase nor protein kinase C is responsible for the alpha 1-adrenoceptor- and purinoceptor-mediated stimulation of the antiport.
WDR1 colocalizes with ADF and actin in the normal and noise‐damaged chick cochlea (2002)

Auditory hair cells of birds, unlike hair cells in the mammalian organ of Corti, can regenerate following sound-induced loss. We have identified several genes that are upregulated following such an insult. One gene, WDR1, encodes the vertebrate homologue of actin-interacting protein 1, which interacts with actin depolymerization factor (ADF) to enhance the rate of actin filament cleavage. We examined WDR1 expression in the developing, mature, and noise-damaged chick cochlea by in situ hybridization and immunocytochemistry. In the mature cochlea, WDR1 mRNA was detected in hair cells, homogene cells, and cuboidal cells, all of which contain high levels of F-actin. In the developing inner ear, WDR1 mRNA was detected in homogene cells and cuboidal cells by embryonic day 7, in the undifferentiated sensory epithelium by day 9, and in hair cells at embryonic day 16. We also demonstrated colocalization of WDR1, ADF, and F-actin in all three cell types in the normal and noise-damaged cochlea. Immediately after acoustic overstimulation, WDR1 mRNA was seen in supporting cells. These cells contribute to the structural integrity of the basilar papilla, the maintenance of the ionic barrier at the reticular lamina, and the generation of new hair cells. These results indicate that one of the immediate responses of the supporting cell after noise exposure is to induce WDR1 gene expression and thus to increase the rate of actin filament turnover. These results suggest that WDR1 may play a role either in restoring cytoskeletal integrity in supporting cells or in a cell signaling pathway required for regeneration.
383: Which Definition of PSA Doubling Time or Velocity best Predicts Outcome after Radical Prostatectomy? (2007)

You have accessJournal of UrologyModerated Poster 12, Sunda~May20,2007 1:00 - 3:00 pm1 Apr 2007383: Which Definition of PSA Doubling Time or Velocity best Predicts Outcome after Radical Prostatectomy? Matthew F. O'Brien, Paul A. Feam, Brandon Smith, Jason Stasi, Angel M. Serio, Andrew J. Vickers, James A. Eastham, and Hans G. Lilja Matthew F. O'BrienMatthew F. O'Brien More articles by this author , Paul A. FeamPaul A. Feam More articles by this author , Brandon SmithBrandon Smith More articles by this author , Jason StasiJason Stasi More articles by this author , Angel M. SerioAngel M. Serio More articles by this author , Andrew J. VickersAndrew J. Vickers More articles by this author , James A. EasthamJames A. Eastham More articles by this author , and Hans G. LiljaHans G. Lilja More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(18)30636-0AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "383: Which Definition of PSA Doubling Time or Velocity best Predicts Outcome after Radical Prostatectomy?." The Journal of Urology, 177(4S), p. 127 © 2016 by American Urological AssociationFiguresReferencesRelatedDetails Volume 177Issue 4SApril 2007Page: 127 Advertisement Copyright & Permissions© 2016 by American Urological AssociationMetricsAuthor Information Matthew F. O'Brien More articles by this author Paul A. Feam More articles by this author Brandon Smith More articles by this author Jason Stasi More articles by this author Angel M. Serio More articles by this author Andrew J. Vickers More articles by this author James A. Eastham More articles by this author Hans G. Lilja More articles by this author Expand All Advertisement PDF downloadLoading ...
cDNA‐AFLP analysis unravels a genome‐wide hrpG‐regulon in the plant pathogen Xanthomonas campestris pv. vesicatoria (2001)

The Hrp type III protein secretion system is essential for pathogenicity of the Gram-negative plant pathogen Xanthomonas campestris pv. vesicatoria. Expression of the hrp gene cluster is controlled by HrpG, a two-component response regulator, and HrpX, an AraC-type transcriptional activator. Using the cDNA-AFLP technique, 30 hrpG-induced (hgi) and five hrpG-repressed (hgr) cDNA fragments were identified, defining a large hrpG-regulon in X. campestris pv. vesicatoria. Expression of most genes in the hrpG-regulon was dependent on hrpX. Seven cDNA fragments map to the known hrp gene cluster and flanking regions. All other genes appear to be scattered over the chromosome and endogenous plasmids. Sequence analysis identified genes encoding putative extracellular proteases, a putative transcriptional regulator and XopJ and XopB (Xanthomonas outer proteins), homologues of YopJ from Yersinia spp. and the avirulence protein AvrPphD of Pseudomonas syringae respectively. XopB is secreted by the Hrp type III secretion system. Analysis of deletion mutants in several hgi genes revealed a new virulence locus. This study demonstrates that cDNA-AFLP is a powerful tool to study prokaryotic transcriptomes and to identify genes contributing to Xanthomonas virulence and putative effector proteins.