gebhart/patrepeval huggingface dataset:
Intermixing at nickel-porous zirconia junctions (1986)

This acute model yielded sustained atrial fibrillation in approximately 55% of the animals. Cardioversion of atrial fibrillation in sheep is possible with very low energy requirements using transvenous electrode systems (50% successful energy of 1.3 +/- 0.4 J for the 3/3-msec biphasic waveform with a right-to-left lead system). The biphasic waveform had the lowest defibrillation requirements of any waveforms tested, and right-to-left lead systems resulted in lower defibrillation requirements than totally right-sided lead systems. Also, lead systems that probably generated high potential gradients near the sinoatrial and atrioventricular node areas resulted in more frequent episodes of postshock conduction disturbances. Furthermore, synchronization of the shock to the QRS was vital to avoid potentially lethal postshock ventricular arrhythmias...
Design and evaluation of a reconfigurable optical add-drop multiplexer with flexible wave-band routing in SDM networks (2022)

Space-division multiplexing (SDM) is expected to increase the capacity of photonic networks. Reconfigurable optical add-drop multiplexers (ROADMs) for SDM-based networks must have high scalability in terms of port count. However, the ROADM architecture adopted in present networks cannot support large numbers of ports due to the limited port count of wavelength-selective switches. In this paper, we propose a ROADM architecture composed of space switches and wavelength-routing switches. Space switches have lower per-port cost than wavelength-routing switches. However, space switches cannot route optical paths on a wavelength basis, unlike wavelength-routing switches. By combining these two types of switches, the ROADM port count can be cost-effectively expanded virtually unlimitedly. Numerical simulations show the routing performance of our ROADM; they elucidate that the maximum fiber increment penalty is about 5% compared to the reference scheme. Experiments detail the transmission performance of the ROADM, where 32 Gbaud DP-4QAM/8QAM/16QAM signals are aligned on a 50 GHz grid, are examined.
High-speed liquid crystal fiber Fabry-Perot tunable filter (1996)

We report a liquid-crystal fiber Fabry-Perot tunable filter (LC-FFP-TF) with fast tuning (<33 μs) over a 0.4-12 nm wavelength range centered at 1550 nm, at a temperature of 35/spl deg/C, using a commercially available electroclinic LC material, BDH764E. Typically, the fall time of the LC-FFP-TF is over an order of magnitude shorter than the rise time. For the first time to our knowledge, switching between two discrete wavelengths was demonstrated using an LC-FFP-TF.
Buccolingual Implant Position and Vertical Abutment Finish Line Geometry (2015)

The surgical and prosthetic concepts described in this article allow the clinician to recreate a pleasant and natural-looking esthetics while the patient can easily maintain a proper hygiene around the implant. However, despite the promising clinical results obtained by the authors, further clinical and randomized researches should be realized to validate the above-proposed approach.
Iminosugars counteract the downregulation of the interferon γ receptor by dengue virus (2019)

The antiviral mechanism of action of iminosugars against many enveloped viruses is hypothesized to be a consequence of misfolding of viral N-linked glycoproteins through inhibition of host endoplasmic reticulum α-glucosidase enzymes. Iminosugar treatment of dengue virus (DENV) infection results in reduced secretion of virions and hence lower viral titres in vitro and in vivo. We investigated whether iminosugars might also affect host receptors important in DENV attachment and uptake and immune responses to DENV. Using a primary human macrophage model of DENV infection, we investigated the effects of maturation with IL-4, DENV-infection and treatment with N-butyl-1-deoxynojirimycin (NB-DNJ) or N-(9-methoxynonyl)-1-DNJ (MON-DNJ) on expression of 11 macrophage receptors. Whereas iminosugars did not affect surface expression of any of the receptors examined, DENV infection significantly reduced surface IFNγ receptor amongst other changes to total receptor expression. This effect required infectious DENV and was reversed by iminosugar treatment. Treatment also affected signalling of the IFNγ receptor and TNFα receptor. In addition, iminosugars reduced ligand binding to the carbohydrate receptor-binding domain of the mannose receptor. This work demonstrates that iminosugar treatment of primary macrophages affects expression and functionality of some key glycosylated host immune receptors important in the dengue life cycle.
Phase I trial of zirconium 89 (Zr89) radiolabeled J591 in metastatic castration-resistant prostate cancer (mCRPC). (2013)

31 Background: Presently, there are no means of accurately and reproducibly imaging bone metastases for patients with mCRPC. We are investigating a prostate-specific imaging method that can directly visualize metastases using PSMA as a target and Zr89 radiolabeled anti-PSMA antibody J591 as a tracer. Following the FDA roadmap for biomarker development, we report the first human data on the tracer and its preliminary analytic validation. Methods: 5 mCi of Zr89-J591 was administered intravenously and 4 serial PET/CT scans were obtained within the following time intervals post-injection: 2-4 hours, 18-30 hours, 2-5 days, and 6-7 days. Pharmacokinetic (PK) sampling occurred at 5, 30, and 60 minutes post-injection, and at each PET scan. Patients underwent standard cross-sectional imaging, bone scintigraphy, and FDG PET scanning. Metastatic sites were biopsied in order of preference: Zr89-J591 and FDG positivity, then Zr89-J591 and FDG mismatch, and lastly standard imaging and any PET mismatch. Results: Ten patients were scanned, 132 Zr89-J591 positive sites were detected, and 12 lesions (5 bone, 6 node, 1 lung) in 8 patients were biopsied. Tissue correlation: 12/12 biopsies were positive for cancer. 11/12 biopsied lesions (5 bone, 5 node, 1 lung) were positive on Zr89-J591 imaging. 10/12 were positive by standard scans or FDG. 1 lesion was negative by Zr89-J591 PET, but positive by other modalities. Immunokinetics: Blood clearance T 1/2α : 7 +/- 4.5 h (1.1-14 h); T 1/2β : 62 +/- 13 h (51-89 h); Whole body clearance T 1/2 : 219 +/- 48 h (153-317 h). Dosimetry: Maximum retention in liver 8 +/- 1.5 SUV; Kidney 4.1 +/- 0.8 SUV; Tumor 8.2 +/- 6.5 SUV (mean SUV in bone 11.13 +/- 7.57 vs. soft tissue 4.31 +/- 2.59). Optimal time for patient imaging after injection, in terms of tumor to background ratios was 7 +/- 1 days. Conclusions: Zr89-J591 imaging demonstrates excellent tumor localization, pathology correlation, and can demonstrate the presence of tumor even when lesions are negative by standard imaging or FDG PET imaging. PK properties of the tracer have been defined. Use of Zr89-J591 to direct biopsies of metastases provides excellent yield. Further studies to examine reproducibility and post-treatment effects are planned. Clinical trial information: NCT01543659.
ChemInform Abstract: QUANTITATIVE EVALUATION OF THE REACTIVITY OF ALKYLATING AGENTS (1980)

Abstract Durch konkurrierende Reaktion insbesondere der Chlormethylbenzoate (I) und des Esters (II) als Standard mit dem Chinuclidin (III) wird auf der Basis des NMR‐spektroskopisch ermittelten Produktverhältnisses (IV)/(V) eine Reaktivitätsskala für die "weichen" Alkylierungsmittel (I) erhalten, wobei sowohl die Reaktivitäten als auch die Methylen‐ "C‐Verschiebungen der Ester (I) mit Taft‐o°‐Werten gut korrelieren.
A call for mentoring of medical students in the backdrop of integrated curriculum. (2014)

Students are aware of benefits of mentoring and seek informal mentoring from faculty and senior colleagues. There is need of formal mentoring program at institutional level.
A Non-Optimization-Based Dynamic Path Planning for Autonomous Obstacle Avoidance (2022)

This article presents a non-optimization-based framework for path planning and tracking for evasive maneuvers in autonomous cars. The framework exploits a two-layer approach where a path planner generates a reference trajectory that is then tracked by a path-tracking controller. A nested curvature preview controller (CPC) implements path tracking. In this article, we show how to describe the closed-loop performance of the controller. The quantification of the closed-loop performance in the frequency domain guides the generation of the evasive path. In this way, the algorithm generates a path that avoids the obstacle (if possible) accounting for both static and dynamic constraints. The proposed framework, thus, provides a non-optimization-based way to integrate the characteristics of the path tracker in the path-planner algorithm, thus avoiding the need to define cost functions and use the third-party optimizers. This article validates the proposed evasive maneuver strategy in simulation and on an instrumented vehicle. First, we test the trajectory tracker, showing that it tracks aggressive trajectories (with a lateral acceleration close to 1 g) with an error smaller than 30 cm. Subsequently, we integrate the curvature preview with the path generator and show the joint generation-tracking performance in two different scenarios.
Daily Electrical Muscle Stimulation Enhances Functional Recovery Following Nerve Transection and Repair in Rats (2014)

Our results demonstrated that EMS using a moderate stimulation paradigm immediately following nerve transection and repair enhances electrophysiological and behavioral recovery.
Low-Fat Diet and Exercise Preserve eNOS Regulation and Endothelial Function in the Penis of Early Atherosclerotic Pigs: A Molecular Analysis (2008)

Low-fat diet and chronic exercise preserve endothelial function in the pig penis by sustaining active eNOS in its dimeric form and by limiting eNOS interaction with its negative regulator caveolin-1.
Supplementary Tables S1-5 from Resistance to CDK2 Inhibitors Is Associated with Selection of Polyploid Cells in <i>CCNE1</i>-Amplified Ovarian Cancer (2023)

&lt;p&gt;Supplementary Tables S1-5 - PDF filr 185K, Supplementary Table S1: Essentiality of CDKs in CCNE1 amplified cell lines; Supplementary Table S2: GeneGo gene expression pathways enriched in PHA-533533 resistant cell lines; Supplementary Table S3: Copy number changes identified by SNP microarray in PHA-533533 resistant cell lines; Supplementary Table S4: Gene Set Enrichment Analysis (GSEA) of positional gene sets enriched in amplified or deleted genes specific to PHA-533533 resistant cells; Supplementary Table S5: OVCAR-3 cell line and derivative resistant line karyotypes&lt;/p&gt;
Bivalirudin as a foundation anticoagulant in peripheral vascular disease: a safe and feasible alternative for renal and iliac interventions. (2003)

Bivalirudin is a safe and feasible alternative anticoagulant in renal and iliac PPI and may offer decreased sheath removal time, time to ambulation and LOS. A larger prospective randomized multicenter trial is warranted.
Increased mitochondrial metabolism profile is required for immunologic function in age associated B cells (2022)

Abstract Specific metabolic programs are activated by immune cells to fulfill their functional roles. Age-associated B cells (ABCs) are a subset of B cells that are identified by expression of non-canonical B lineage marker, CD11c. An expansion of ABCs has been identified in various autoimmune diseases, including systemic lupus erythematosus (SLE). They have many distinct properties, such as capacity to stimulate T cells and the ability to secrete autoreactive antibodies when they are stimulated in vitro. The metabolic pathways that support these functions are unknown. Using mouse models of human SLE, we confirmed ABCs induce T cell activation effectively and these activated T cells acquire follicular helper T (Tfh) cell phenotypes, including BCL6 expression, up-regulation of surface expression of CXCR5 and PD-1 and IL-21 production. Metabolically, ABCs are bioenergetically more active than follicular (FO) B cells, with higher rates of glycolysis and oxidative phosphorylation. They have increased mitochondrial activation with an increased production of reactive oxygen species (mtROS). Treatment of ABCs with mitochondrial complex I inhibitor or mtROS scavenger leads to a selective loss of their capacity to induce Tfh differentiation but not T cell activation or proliferation. The aberrant activation of mitochondrial metabolism and mtROS production was also consistently observed in ABCs from SLE patients as well. Therefore, ABCs have evolved a distinct metabolism adapted to their specific functional properties. Supported by NIH NIAMS R01 AR075565 NIH NIAMS U19 AI144306
Effects of Injection Molding and Storage Conditions on Polypropylene Copolymer (2017)

Abstract This work focused on studying the effects of injection molding parameters (cooling time and melt temperature) and storage conditions (annealing versus cooling) on the thermal, mechanical, and optical properties of an ethylene-propylene copolymer made with and without nucleating agents. Preform properties (optical, crystallinity) affect the consistency of the preforms’ reheating via infrared lamps before stretch blow molding. The analysis of detailed preform properties as a result of both injection molding processing and postmolding treatment or preform storage conditions was lacking to correlate with the blow molding performance. This study confirmed that postmolding aging is a significant factor that affects material properties and therefore suggested subsequent blow molding. It also suggested that shorter injection molding cooling time helps to minimize the effects of aging and storage conditions. As expected, with the inclusion of the nucleating agent, clarity, yield strength, and density of the material improved.
Activation by Point Mutation of Ki-ras Gene Occurring in Transfected Human Normal dna (1988)

A mutation-activated human Ki-ras gene was detected in NIH 3T3 cells transfected with high molecular weight DNA extracted from peripheral blood leukocytes of a healthy blood donor. A deoxyguanosine at position 35 of the first exon was substituted by deoxythymidine. Nevertheless, cloning and sequencing of seven independent Ki-ras first exons, isolated from the same human genomic DNA used to transfect NIH 3T3 cells, failed to reveal the expected point mutation. Since transfected DNA is susceptible to mutagenesis in mammalian cells, we hypothesize that a base substitution occurred during the transfection assay.
Aspergillus nidulans: a Model for Elucidation ofAspergillus fumigatusSecondary Metabolism (2014)

This chapter reviews the contributions made by Aspergillus nidulans to the understanding of fungal secondary metabolism and the ways in which advances in our understanding of this species have spurred parallel studies of Aspergillus fumigatus. The most thorough insight into fungal secondary metabolite regulation has arisen from studies of the mycotoxin sterigmatocystin (ST) and the antibiotic penicillin in A. nidulans. With possibly the exception of the penicillin metabolic cluster, the most thoroughly examined fungal secondary-metabolite gene clusters are those involved in mycotoxin biosynthesis, particularly the aflatoxin (AF) and ST biosynthetic clusters found in several Aspergillus spp. Coordinate regulation is largely explained by transcriptional control by pathway-specific regulatory factors (e.g., aflR) and global regulatory proteins including transcription factors mediating environmental signals (pH, carbon, and nitrogen) and the cluster-specific methylase, LaeA. Specific oxylipins, e.g., various prostaglandins, are ligands to G-protein-coupled receptors (GPCR), which are important in inflammatory and immune responses in mammals. Interestingly, recent studies have identified three A. nidulans GPCR that impact asexual and sexual spore production, and efforts are under way to determine if Ppo products may be potential ligands for these receptors. PGs, along with leukotrienes, comprise a class of oxylipins called eicosanoids formed from C20 fatty acids (dihomo-γ-linolenic acid, arachidonic acid, and eicosanopentaenoic acid). Initial studies of laeA and ppo function in A. fumigatus indicate a potent role for these secondarymetabolite genes in pathogenesis. A thorough understanding of the function of these and other secondary metabolism genes may assist in the development of future therapeutics.
Isocitrate lyase of Ashbya gossypii – transcriptional regulation and peroxisomal localization (1999)

The isocitrate lyase-encoding gene AgICL1 from the filamentous hemiascomycete Ashbya gossypii was isolated by heterologous complementation of a Saccharomyces cerevisiae icl1d mutant. The open reading frame of 1680 bp encoded a protein of 560 amino acids with a calculated molecular weight of 62584. Disruption of the AgICL1 gene led to complete loss of AgIcl1p activity and inability to grow on oleic acid as sole carbon source. Compartmentation of AgIcl1p in peroxisomes was demonstrated both by Percoll density gradient centrifugation and by immunogold labeling of ultrathin sections using specific antibodies. This fitted with the peroxisomal targeting signal AKL predicted from the C-terminal DNA sequence. Northern blot analysis with mycelium grown on different carbon sources as well as AgICL1 promoter replacement with the constitutive AgTEF promoter revealed a regulation at the transcriptional level. AgICL1 was subject to glucose repression, derepressed by glycerol, partially induced by the C2 compounds ethanol and acetate, and fully induced by soybean oil.
The Concentration and Separation Of Blood Components Using Acoustic Radiation Force (2013)

Abstract The concentration of whole blood has a wide spectrum of medical uses, especially in blood transfusions for patients suffering acute blood loss from trauma or surgery. However, current methods rely on centrifugation, which has a tendency to cause fragmentation and deformation of cells. Acoustic standing waves are considered to be a gentler means to concentrate blood cells, but generally suffer from flow rate limitations of &gt;103fold, functioning only on the microscale. To address this issue, we have developed a novel ultrasound technology that works at the macroscale, enabling it to process flow rates that would typically be required to handle medically relevant volumes. In this study, we have applied the principles of acoustic radiation at the macroscale to re-concentrate 10X diluted porcine blood by trapping the blood cells within a standing wave, resulting in the clumping of cells and platelets, ultimately leading to enhanced gravitational settling. Complete blood counts were measured with a VetScan HM5 hematology analyzer. Using an acoustic force generated by power levels up to 25 Watts, no lysing was observed in any of the experiments. The inlet flow rate through the device was 16 ml/min, and the concentrate flow rate of the blood components was typically 1 ml/min. The transducer was a 2 MHz PZT-8 operating at 10 W, and it was oriented to create an acoustic standing wave perpendicular to the flow direction. Results indicate successful capture of red blood cells, white blood cells, and platelets with separation efficiencies in excess of 90% in a single pass. Furthermore, the blood components were all reconcentrated back to levels similar to those in whole blood. Subsequent experiments and conditions have allowed for the separation of blood components, suggesting that the future development of acoustic methods to perform blood re-concentration procedures such as erythropheresis, leukapheresis and plateletpheresis in a gentler manner than current methods holds great promise. With almost 15 million transfusions performed each year in the United States, this technology has the potential to make a significant impact on the medical community, particularly for treatment of critically ill patients. Additionally, the novel technology has also demonstrated performance in separating lipid particles from blood, thus indicating its utility for reducing the occurrence of lipid microemboli during re-transfusion of shed blood in cardiopulmonary bypass surgeries, which have been linked with post-operative neurocognitive complications. As a result, the novel acoustophoretic technology may become a general purpose platform for achieving separation in a variety of biomedical applications. Disclosures: Mealey: Flo Design Sonics: Employment. Lipkens:Flo Design Sonics: Equity Ownership.
Progress Report on Pump Limiter Developments for the TEXTOR Tokamak: ALT-I (1984)

A collaborative program is underway to field a comprehensive pump limiter experimental program on TEXTOR: Advanced Limiter Test-I (ALT-I). Either of two interchangeable limiter modules may be attached to an insertion/rotation mechanism to direct particles to the ∼1 m3 pumping chamber. Pumping is provided primarily by a solid getter assembly at ∼20,000 1/sec. Variation of geometric dimensions and gas puffing in the modules will allow the study of plasma and neutral interaction in differing recycle regimes. Multiple diagnostic systems are incorporated into the pump limiter design for use in conjunction with TEXTOR plasma diagnostics. Initial experiments are scheduled for December 1983.
Laparoscopic Retropubic Auto-Augmentation of the Bladder (1995)

The small capacity or contracted bladder is a difficult management problem. The goal of bladder augmentation is to create a storage structure with an adequate capacity and low pressure. Bladder auto-augmentation creates a large bladder diverticulum by partially excising the detrusor muscle. We report our initial experience with an extraperitoneal approach to laparoscopic auto-augmentation in a patient with a small contracted bladder. This is a technically feasible operation but longer clinical followup is necessary to determine its durability in the management of the small contracted bladder.
Chemokine‐Glycosaminoglycan Binding: Specificity for CCR‐11 Ligand Binding to Highly Sulfated Oligosaccharides using FTICR MS (2006)

Chemokine ligands that are specific to the CCR11 receptor include the proteins MCP1, MCP2, MCP3, MCP4 and Eotaxin. These ligands form non‐covalent complexes with specific sulfated saccharides prior to binding to the receptor. Mass Spectrometry has been used to show that when a library of glycosaminoglycans (GAG) octasaccharides is incubated with the various ligands, very specific interactions occur between the chemokine and octasaccharides. Experiments involving the non‐covalent complex between GAG and chemokine show not only saccharide specificity but also isomeric specificity. FTICR mass spectrometry was used to analyze a library of differentially sulfated octasaccharides (GAG's) that was incubated with various chemokine ligands. In the incubation of the octasaccharide library with the chemokine MCP‐1, it was found that those GAG octasaccharides having 10, 11 and 12 sulfates were found to be specific binders to MCP‐1. Additionally, the chemokines were observed to multimerize to dimers and tetramers during the GAG binding process. This data from the non‐covalent complexes supported previous results, which indicated that GAG binding to chemokines initiates a multimerization process of the chemokines. Dimerization of the protein upon specific GAG binding is clearly observed in the mass spectra, but only for some of the CCR11 ligands; i.e. MCP1 and 2 but not 3, 4 or eotaxin. It is believed that this multimerization is critical for chemokine binding to the CCR11 receptor as well as being responsible for subsequent receptor activity.