gebhart/patrepeval huggingface dataset:
Angiotensin-II Type 1 Receptor–Mediated Hypertension in D 4 Dopamine Receptor–Deficient Mice (2005)

Dopamine receptors are important in systemic blood pressure regulation. D4 receptors are expressed in the kidney and brain, but their role in cardiovascular regulation is unknown. In pentobarbital-anesthetized mice, systolic and diastolic blood pressures were elevated in sixth-generation D4 receptor-deficient (D4(-/-)) mice and in tenth-generation D4(-/-) mice compared with D4 wild-type (D4(+/+)) littermates. The conscious blood pressures measured via a chronic arterial (femoral) catheter or telemetry (carotid) were also higher in D4(-/-) mice than in D4 littermates. Basal renal and plasma renin concentrations were similar in the 2 mouse strains. The protein expression of angiotensin II type 1 receptor was increased in homogenates of kidney (330+/-53%, n=5) and brain (272+/-69%, n=5) of D4(-/-) mice relative to D4(+/+) mice (kidney: 100+/-12%, n=5; brain: 100+/-32%, n=5). The expression of the receptor in renal membrane was also increased in D4(-/-) mice (289+/-28%, n=8) relative to D4(+/+) mice (100+/-14%, n=10). In contrast, the expression in the heart was similar in the 2 strains. Bolus intravenous injection of angiotensin II type 1 receptor antagonist losartan initially decreased mean arterial pressures to a similar degree in D4(-/-) and D4(+/+) littermates. However, the hypotensive effect of losartan dissipated after 10 minutes in D4(+/+) mice, whereas the effect persisted for >45 minutes in D4(-/-) mice. We conclude that the absence of the D(4) receptor increases blood pressure, possibly via increased angiotensin II type 1 receptor expression.
Target tracking with binary proximity sensors (2009)

We explore fundamental performance limits of tracking a target in a two-dimensional field of binary proximity sensors, and design algorithms that attain those limits while providing minimal descriptions of the estimated target trajectory. Using geometric and probabilistic analysis of an idealized model, we prove that the achievable spatial resolution in localizing a target's trajectory is of the order of 1/ρ R , where R is the sensing radius and ρ is the sensor density per unit area. We provide a geometric algorithm for computing an economical (in descriptive complexity) piecewise linear path that approximates the trajectory within this fundamental limit of accuracy. We employ analogies between binary sensing and sampling theory to contend that only a “lowpass” approximation of the trajectory is attainable, and explore the implications of this observation for estimating the target's velocity. We also consider nonideal sensing, employing particle filters to average over noisy sensor observations, and geometric geometric postprocessing of the particle filter output to provide an economical piecewise linear description of the trajectory. In addition to simulation results validating our approaches for both idealized and nonideal sensing, we report on lab-scale experiments using motes with acoustic sensors.
The CD85/LIR-1/ILT2 Inhibitory Receptor Is Expressed by All Human T Lymphocytes and Down-Regulates Their Functions (2000)

The inhibitory molecule CD85/LIR-1/ILT2 has been detected previously on the surface of a small proportion of T lymphocytes. In this study, evidence is provided that, although only a fraction of CD3+ cells are stained by mAb specific for CD85/LIR-1/ILT2 on their surface, this inhibitory receptor is present in the cytoplasm of all T lymphocytes, and that it is detectable on the surface of all T cell clones by the M402 mAb. Biochemical analyses further demonstrate that CD85/LIR-1/ILT2 is present in all T clones analyzed, and that the protein is tyrosine-phosphorylated. Expression of mRNA coding for CD85/LIR-1/ILT2 has been assessed by RT-PCR. Notably, in the NKL cell line and in one T cell clone, amplification of the messenger required 30 cycles only, whereas, in other T cell clones, an amplification product was detected by increasing the number of cycles. CD85/LIR-1/ILT2 inhibits CD3/TCR-mediated activation in both CD4+ and CD8+ clones, and it down-regulates Ag recognition by CD8+ cells in a clonally distributed fashion. Addition of anti-ILT2 HP-F1 mAb in the cytolytic assay enhances target cell lysis mediated by Ag-specific CTL. This could be due to interference of the mAb with receptor/ligand interactions. In contrast, HP-F1 mAb cross-linking triggers inhibitory signals that reduce cytotoxicity. CD85/LIR-1/ILT2 also controls responses to recall Ags and, in low responders, its engagement sharply increases T cell proliferation. The inhibitory function of the molecule is also confirmed by its ability to reduce CD3/TCR-induced intracellular Ca2+ mobilization.
Inside Cover: Generation and Reactions of an Unsubstituted N‐Heterocyclic Carbene Boryl Anion (Angew. Chem. Int. Ed. 48/2010) (2010)

Easy access to a new carbene boryl anion has been discovered by a collaborative team of three research groups, from Paris and Pittsburgh. In their Communication on page 9166 ff., M. Malacria, D. P. Curran et al. describe the preparation of an N-heterocyclic carbene boryl anion through reductive lithiation of a boryl iodide. This anion can be trapped at boron with a variety of electrophiles to give unusual carbene borane products.
Numerical simulation of suture tension dependent on fiber material and puncture modalities (2011)

Most surgical interventions require joining of soft tissue, and suturation is the most common approach. While high suture tension may result in ischemia or even necrosis, a loose structure holds the risk of hemorrhage of the wound edges, causing postoperative complications and a potential necessity for reoperation. Estimating and controlling the tension within the suture is therefore a crucial factor for suture stability and thus successful treatment. In this study a numerical evaluation of different fiber materials and puncture modalities is presented. In total, 120 combinations of fiber material and number and distance of punctures are compared in terms of maximum shear rates representing suture tension. The results indicate that Vicryl fibers provide lower suture tension compared to PDS, and that an increased number of punctures decreases suture tension. Results are in good agreement with experimental studies. The presented method can be used to analyze various suture techniques and thus help to achieve successful treatment.
Targeting SAMHD1 with hydroxyurea in first‐line cytarabine‐based therapy of newly diagnosed acute myeloid leukaemia: Results from the HEAT‐AML trial (2022)

Targeted inhibition of SAMHD1 by the addition of hydroxyurea to conventional AML therapy is safe and appears efficacious within the limitations of the small phase 1 patient cohort. These results need to be corroborated in a larger study.
ChemInform Abstract: MATRIX ISOLATION STUDIES OF THE REACTIONS OF SILICON ATOMS. II. INFRARED SPECTRUM AND STRUCTURE OF MATRIX‐ISOLATED FLUOROSILYLENE: HSIF (1982)

Abstract In einer Matrix aus festem Argon reagiert Si spontan mit HF und DF unter Bildung vonHSiF und DSiF.
On the Origin of Sinter‐Resistance and Catalyst Accessibility in Raspberry‐Colloid‐Templated Catalyst Design (2021)

Abstract Nanoparticle (NP) sintering is a major cause of the deactivation of supported catalysts. Raspberry‐colloid‐templated (RCT) catalysts are an emerging class of materials that show an unprecedented level of sinter‐resistance and exhibit high catalytic activity. Here a comprehensive study of the origin of NP stability and accessibility in RCT catalysts using theoretical modeling, 3D electron microscopy, and epitaxial overgrowth is reported. The approach is showcased for silica‐based RCT catalysts containing dilute Pd‐in‐Au NPs previously used in hydrogenation and oxidation catalysis. Modeling of the contact line of the silica precursor infiltrating into the assembled raspberry colloids suggests that a large part of the particles must be embedded into silica, which is confirmed by quantitative visualization of &gt;200 individual NPs by dual‐axis electron tomography. The RCT catalysts have a unique structure in which all NPs reside at the pore wall but have &gt;50% of their surface embedded in the matrix, giving rise to the strongly enhanced thermal and mechanical stability. Importantly, epitaxial overgrowth of Ag on the supported NPs reveals that not only the NP surface exposed to the pore but the embedded interface as well remained chemically accessible. This mechanistic understanding provides valuable guidance in the design of stable catalytic materials.
Interdisciplinary Graduate Training in Teaching Labs (2012)

Intensive, short-term courses meld students and faculty and new techniques in pursuit of genuine research questions.
A High-Throughput Screen for Teratogens Using Human Pluripotent Stem Cells (2013)

There is need in the pharmaceutical and chemical industries for high-throughput human cell-based assays for identifying hazardous chemicals, thereby reducing the overall reliance on animal studies for predicting the risk of toxic responses in humans. Despite instances of human-specific teratogens such as thalidomide, the use of human cell-teratogenicity assays has just started to be explored. Herein, a human pluripotent stem cell test (hPST) for identifying teratogens is described, benchmarking the in vitro findings to traditional preclinical toxicology teratogenicity studies and when available to teratogenic outcomes in humans. The hPST method employs a 3-day monolayer directed differentiation of human embryonic stem cells. The teratogenic risk of a compound is gauged by measuring the reduction in nuclear translocation of the transcription factor SOX17 in mesendodermal cells. Decreased nuclear SOX17 in the hPST model was strongly correlated with in vivo teratogenicity. Specifically, 71 drug-like compounds with known in vivo effects, including thalidomide, were examined in the hPST. A threshold of 5 μM demonstrated 94% accuracy (97% sensitivity and 92% specificity). Furthermore, 15 environmental toxicants with physicochemical properties distinct from small molecule pharmaceutical agents were examined and a similarly strong concordance with teratogenicity outcomes from in vivo studies was observed. Finally, to assess the suitability of the hPST for high-throughput screens, a small library of 300 kinase inhibitors was tested, demonstrating the hPST platform's utility for interrogating teratogenic mechanisms and drug safety prediction. Thus, the hPST assay is a robust predictor of teratogenicity and appears to be an improvement over existing in vitro models.
주사용 미세유량 조절기 설계와 제작 (2004)

A new microflow rate controller for medical injection was developed and evaluated. The flow rate was controlled by changing the friction depth as well as the friction length of the micro-channel. A precise micro-fabrication of the micro-channel was requested for an accurate flow control. The friction depth was inversely proportional to the friction length, which gives a linear flow control to the channel length. The channel groove was fabricated with a plastic material. A rubber containing silicone oil was covered over the groove, which satisfies both lubrication and leakage prevention. The flow controller was validated by performing the numerical simulation and experiment. A good agreement was shown between computation and experiment.
High intensity focused ultrasound: Physical principles and devices (2007)

High intensity focused ultrasound (HIFU) is gaining rapid clinical acceptance as a treatment modality enabling non-invasive tissue heating and ablation for numerous applications. HIFU treatments are usually carried out in a single session, often as a day case procedure, with the patient either fully conscious, lightly sedated or under light general anaesthesia. A major advantage of HIFU over other thermal ablation techniques is that there is no necessity for the transcutaneous insertion of probes into the target tissue. The high powered focused beams employed are generated from sources placed either outside the body (for treatment of tumours of the liver, kidney, breast, uterus, pancreas and bone) or in the rectum (for treatment of the prostate), and are designed to enable rapid heating of a target tissue volume, while leaving tissue in the ultrasound propagation path relatively unaffected. Given the wide-ranging applicability of HIFU, numerous extra-corporeal, transrectal and interstitial devices have been designed to optimise application-specific treatment delivery. Their principle of operation is described here, alongside an overview of the physical mechanisms governing HIFU propagation and HIFU-induced heating. Present methods of characterising HIFU fields and of quantifying HIFU exposure and its associated effects are also addressed.
Relaxin Receptor RXFP1 and RXFP2 Expression in Ligament, Tendon, and Shoulder Joint Capsule of Rats (2016)

Numerous musculoskeletal disorders are caused by thickened ligament, tendon stiffness, or fibrosis of joint capsule. Relaxin, a peptide hormone, can exert collagenolytic effect on ligamentous and fibrotic tissues. We hypothesized that local injection of relaxin could be used to treat entrapment neuropathy and adhesive capsulitis. Because hormonal effect depends on the receptor of the hormone on the target cell, it is important to confirm the presence of such hormonal receptor at the target tissue before the hormone therapy is initiated. The aim of this study was to determine whether there were relaxin receptors in the ligament, tendon, and joint capsular tissues of rats and to identify the distribution of relaxin receptors in these tissues. Transverse carpal ligaments (TCLs), inguinal ligaments, anterior cruciate ligaments (ACLs), Achilles tendons, and shoulder joint capsules were obtained from male Wistar rats. Western blot analysis was used to identify relaxin receptor isoforms RXFP1 and RXFP2. The distribution of relaxin receptors was determined by immunohistochemical staining. The RXFP1 isoform was found in all tissues examined. The RXFP2 isoform was present in all tissues but the TCLs. Its expression in ACLs tissues was relatively weak compared to that in other tissues. Our results revealed that RXFP1 and RXFP2 were distributed in distinctly different patterns according to the type of tissue (vascular endothelial cells, fibroblast-like cells) they were identified.
The FGFR1 Inhibitor PD 173074 Selectively and Potently Antagonizes FGF‐2 Neurotrophic and Neurotropic Effects (2000)

Basic fibroblast growth factor (FGF-2) promotes survival and/or neurite outgrowth from a variety of neurons in cell culture and regenerative processes in vivo. FGFs exert their effects by activating cell surface receptor tyrosine kinases. FGF receptor (FGFR) inhibitors have not been characterized on neuronal cell behaviors to date. In the present study, we show that the FGFR1 inhibitor PD 173074 potently and selectively antagonized the neurotrophic and neurotropic actions of FGF-2. Nanomolar concentrations of PD 173074 prevented FGF-2, but not insulin-like growth factor-1, support of cerebellar granule neuron survival under conditions of serum/K(+) deprivation; another FGF-2 inhibitor, SU 5402, was effective only at a 1,000-fold greater concentration. Neither PD 173074 nor SU 5402, at 100 times their IC(50) values, interfered with the survival of dorsal root ganglion neurons promoted by nerve growth factor, ciliary neurotrophic factor, or glial cell line-derived neurotrophic factor. PD 173074 and SU 5402 displayed 1,000-fold differential IC(50) values for inhibition of FGF-2-stimulated neurite outgrowth in PC12 cells and in granule neurons, and FGF-2-induced mitogen-activated protein kinase (p44/42) phosphorylation. The two inhibitors failed to disturb downstream signalling stimuli of FGF-2. PD 173074 represents a valuable tool for dissecting the role of FGF-2 in normal and pathological nervous system function without compromising the actions of other neurotrophic factors.
Ultrafiltrazione peritoneale e sindrome cardiorenale: gestione del sovraccarico di fluidi e ruolo del sodio (2019)

Congestion represents a crucial clinical component of both heart failure and cardiorenal syndrome and it has been postulated to modulate heart and kidney cross-link. Diuretic therapy is a corner stone in the treatment patients with heart failure, and renal replacement therapies are mainly used for patients with refractory heart failure who have not reached the worst stages of renal disfunction. Peritoneal dialysis is a home-based therapeutic modality providing both solute clearance and ultrafiltration, together with relief from congestion in decompensated heart failure patients. The following review will focus on sodium removal in refractory decompensated heart failure patients undergoing peritoneal dialysis.&nbsp;(Cardionephrology)
Screening of the epsilon sarcoglycan gene in Tourette syndrome and obsessive compulsive disorder (2008)

Katerberg, Hilga; Cath, Danielle C.; Tijssen, Marina A.J.; van Balkom, Anton J.L.M.; van de Leemput, Yvonne L.C.; den Boer, Johan A.; Heutink, Peter; Baas, Frank Author Information
An Investigation on the Short Channel Effect for 0.1 µm Fully Depleted SOIMOSFET Using Equivalent One Dimensional Model (1995)

An analytical model for the short channel effect for the silicon on insulator metal oxide semiconductor field effect transistor (SOIMOSFET) is proposed. The two dimensional potential problem is reduced to a one dimensional problem, using a virtual electrode which is equivalent to the drain and the source electrodes. The behavior for the short channel effect is also discussed using this model.
Stochastic Engine: Direct Incorporation of Measurements Into Predictive Simulations (2001)

We are creating a new method of combining disparate types of geologic observations and process simulations. Using Bayesian inferencing and an efficient search algorithm, we obtain a consolidated body of knowledge in the form of multiple configurations and parameter values of the system that are consistent with our existing data and process models. In so doing, we effectively estimate the distributions of both individual parameters and system-wide states, and their likelihood of occurrence. This is in contrast with conventional inversion methods, which produce a single deterministic understanding lacking quantitative information about the distribution of uncertainty. We call this combination of probabilistic evaluation and deterministic process simulators the stochastic engine. Our approach allows the investigators to rapidly improve their understanding of system progress, making it particularly valuable for active processes like injection. The Bayesian inferencing is driven by forward process models that predict data values, such as temperature or electrical voltage, for direct comparison to measured field values. We stage the stochastic searches of possible configurations and run the simplest models, such as lithology estimators, at the lower stages. The majority of possible configurations are eliminated from further consideration by the higher stages' more complex models, such as electrical resistance models for geophysical imaging, or flow and transport models for fluid movement. The approach allows for the continuous augmentation of existing data with newly available information to enhance our understanding and reduce the number of high likelihood configurations. This effectively creates a tool capable of dynamically finding models of underground geological systems that are consistent with all available data. The stochastic engine approach will dramatically increase our understanding of large-scale complex systems and the accuracy of predicting their future behavior under natural or man-made conditions.
Multiple Membrane-Cytoplasmic Domain Contacts in the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Mediate Regulation of Channel Gating (2020)

The cystic fibrosis transmembrane conductance regulator (CFTR) is a unique ATP-binding cassette (ABC) ion channel mutated in patients with cystic fibrosis. The most common mutation, deletion of phenylalanine 508 (ΔF508) and many other disease-associated mutations occur in the nucleotide binding domains (NBD) and the cytoplasmic loops (CL) of the membrane-spanning domains (MSD). A recently constructed computational model of the CFTR three-dimensional structure, supported by experimental data (Serohijos, A. W., Hegedus, T., Aleksandrov, A. A., He, L., Cui, L., Dokholyan, N. V., and Riordan, J. R. (2008) Proc. Natl. Acad. Sci. U. S. A. 105, 3256–3261) revealed that several of these mutations including ΔF508 disrupted interfaces between these domains. Here we have used cysteine cross-linking experiments to verify all NBD/CL interfaces predicted by the structural model and observed that their cross-linking has a variety of different effects on channel gating. The interdomain contacts comprise aromatic clusters important for stabilization of the interfaces and also involve the Q-loops and X-loops that are in close proximity to the ATP binding sites. Cross-linking of all domain-swapping contacts between NBDs and MSD cytoplasmic loops in opposite halves of the protein rapidly and reversibly arrest single channel gating while those in the same halves have lesser impact. These results reinforce the idea that mediation of regulatory signals between cytoplasmic- and membrane-integrated domains of the CFTR channel apparently relies on an array of precise but highly dynamic interdomain structural joints.
An Agent-Based Distributed State Estimation Approach for Interconnected Power Systems (2022)

Phasor measurement units (PMUs) are becoming more popular in power systems and most of the distributed state estimation methods are based on the Gaussian measurement noise assumption. However, the PMU measurement noise has been demonstrated to be non-Gaussian. This article presents a distributed multiarea state estimation approach based on the quadratic and exponential functions for power systems to deal with the non-Gaussian measurement noise efficiently. The matrix splitting technique is utilized to implement a robust estimator in a fully distributed scheme. Based on the local measurements and the limited information exchanged between neighboring areas, each local area only estimates its local states and the computation time needed is further reduced. The effective and robust performance of the proposed distributed multiarea state estimation approach is demonstrated using the IEEE 30-, 118-, and 300-bus systems.
ChemInform Abstract: SYNTHESIS AND CHARACTERIZATION OF SOME REVERSIBLE IRON(II) DIOXYGEN CARRIERS OF LACUNAR MACROBICYCLIC LIGANDS AND THEIR REACTIVITIES WITH DIOXYGEN (1984)

Abstract Die makrobicyclischen Fe(II)‐Komplexe (I) werden durch Umsetzung des tetraprotonierten Liganden [H 4 L](PF 6 ) 3 Cl oder [H 4 L][ZnCl 4 ] 2 mit Bis‐[acetonitril oder pyridin]‐Fe(II)‐Chlorid in Gegenwart von Triethylamin in Acetonitril oder Methanol synthetisiert.