gebhart/patrepeval huggingface dataset:
Amine‐functionalized polypyrrole: Inherently cell adhesive conducting polymer (2014)

Electrically conducting polymers (CPs) have been recognized as novel biomaterials that can electrically communicate with biological systems. For their tissue engineering applications, CPs have been modified to promote cell adhesion for improved interactions between biomaterials and cells/tissues. Conventional approaches to improve cell adhesion involve the surface modification of CPs with biomolecules, such as physical adsorption of cell adhesive proteins and polycationic polymers, or their chemical immobilization; however, these approaches require additional multiple modification steps with expensive biomolecules. In this study, as a simple and effective alternative to such additional biomolecule treatment, we synthesized amine-functionalized polypyrrole (APPy) that inherently presents cell adhesion-supporting positive charges under physiological conditions. The synthesized APPy provides electrical activity in a moderate range and a hydrophilic surface compared to regular polypyrrole (PPy) homopolymers. Under both serum and serum-free conditions, APPy exhibited superior attachment of human dermal fibroblasts and Schwann cells compared to PPy homopolymer controls. Moreover, Schwann cell adhesion onto the APPy copolymer was at least similar to that on poly-l-lysine treated PPy controls. Our results indicate that amine-functionalized CP substrates will be useful to achieve good cell adhesion and potentially electrically stimulate various cells. In addition, amine functionality present on CPs can further serve as a novel and flexible platform to chemically tether various bioactive molecules, such as growth factors, antibodies, and chemical drugs.
993: The Cyclopentenone Prostaglandin, 15-Deoxy-Δ12,14-PGJ2, Attenuates the Developmemt of Cyclophosphamide Induced Cystitis (2007)

You have accessJournal of UrologyModerated Poster 30, Monday, May 21, 2007, 1:00 - 3:00 pm1 Apr 2007993: The Cyclopentenone Prostaglandin, 15-Deoxy-Δ12,14-PGJ2, Attenuates the Developmemt of Cyclophosphamide Induced Cystitis Hitoshi Masuda, Kazunori Kihara, Michael B. Chancellor, and Naoki Yoshimura Hitoshi MasudaHitoshi Masuda More articles by this author , Kazunori KiharaKazunori Kihara More articles by this author , Michael B. ChancellorMichael B. Chancellor More articles by this author , and Naoki YoshimuraNaoki Yoshimura More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(18)31221-7AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "993: The Cyclopentenone Prostaglandin, 15-Deoxy-Δ12,14-PGJ2, Attenuates the Developmemt of Cyclophosphamide Induced Cystitis." The Journal of Urology, 177(4S), p. 328 © 2016 by American Urological AssociationFiguresReferencesRelatedDetails Volume 177Issue 4SApril 2007Page: 328 Advertisement Copyright & Permissions© 2016 by American Urological AssociationMetricsAuthor Information Hitoshi Masuda More articles by this author Kazunori Kihara More articles by this author Michael B. Chancellor More articles by this author Naoki Yoshimura More articles by this author Expand All Advertisement PDF downloadLoading ...
Evaluation Process of Digging Performance for Hydraulic Excavator by Bucket Tip Trace (2011)

A high efficiency digging algorithm for hydraulic excavator has not been established, because the relationship between digging control parameters and digging efficiency is too complicated. Therefore, we have investigated how digging efficiency is affected by the digging control parameters. In this paper, the digging efficiency is defined by the scooped soil mass per applied digging energy because it is the one of the most simple equation to show the digging efficiency. A digging test device was developed to reproduce the digging behavior of entire hydraulic excavator with using the pre-programmed bucket tip trace. In addition, a digging simulation model by two dimensional distinct element method (2D-DEM) is developed to clarify the digging efficiency with considering some different digging settings. The simulation results are verified by comparing with the test data using the digging device. The digging simulations were performed in order to investigate how the bucket tip trace and velocity effect the digging efficiency. This study shows digging efficiency can be improved by changing tip traces or digging velocities.
BBMRI-ERIC ELSI Services & Research Q&A on COVID-19 & ELSI (2020)

The world is facing an unprecedented public health challenge with the COVID-19 pandemic; this has necessitated numerous efforts being deployed by governments, health agencies and individual institutions – including in the biobanking community – to develop effective tests, toolkits, treatments and vaccines to stem its spread and help fight it. To achieve this goal, the collection, analysis and timely sharing of samples and related data becomes instrumental in collective global research efforts. Aligned to these efforts is the prevalence for automated data processing and digital technologies such as location data and contact tracing applications as part of the toolkits. In this respect, biobanks are important infrastructures for access to these samples and data. Besides availability, quality and technical infrastructures, also ethical, legal and societal issues (ELSI) are particularly crucial in this regard. Public health ethics, personal data protection, ethics of data sharing, protection of consent and vulnerability as well as compliance issues within international data sharing have gained urgency in COVID-19 research. Against this backdrop, BBMRI-ERIC ELSI Services &amp; Research held a webinar that took a closer look at these issues. The webinar took place on April 24<sup>th</sup>, 2020; the recording is available here. This document not only answers the questions posed during the webinar but ventures into much detail on the topics raised as a result. It also showcases two examples from Germany (presented by Prof. Roland Jahns) and Italy (presented by Prof. Marialuisa Lavitrano), as well as a detailed commentary on contact-tracing-apps from a legal perspective (presented by Gauthier Chassang). The document thus lays the foundation for further discussion on ELSI and COVID-19 in the context of biobanking.
Outcomes of Elderly Patients Undergoing Left Atrial Appendage Closure (2021)

Background Elderly patients have a higher burden of comorbidities that influence clinical outcomes. We aimed to compare in-hospital outcomes in patients ≥80 years old to younger patients, and to determine the factors associated with increased risk of major adverse events (MAE) after left atrial appendage closure. Methods and Results The National Inpatient Sample was used to identify discharges after left atrial appendage closure between October 2015 and December 2018. The primary outcome was in-hospital MAE defined as the composite of postprocedural bleeding, vascular and cardiac complications, acute kidney injury, stroke, and death. A total of 6779 hospitalizations were identified, of which, 2371 (35%) were ≥80 years old and 4408 (65%) were <80 years old. Patients ≥80 years old experienced a higher rate of MAE compared with those aged <80 years old (6.0% versus 4.6%, <i>P</i>=0.01), and this difference was driven by a numerically higher rate of cardiac complications (2.4% versus 1.8%, <i>P</i>=0.09) and death (0.3% versus 0.1%, <i>P</i>=0.05) among individuals ≥80 years old. In patients ≥80 years old, higher odds of in-hospital MAE were observed in women (1.61-fold), and those with preprocedural congestive heart failure (≈2-fold), diabetes (≈1.5-fold), renal disease (≈2.6-fold), anemia (≈2.7-fold), and dementia (≈5-fold). In patients <80 years old, a higher risk of in-hospital MAE was encountered among women (≈1.4-fold) and those with diabetes (≈1.3-fold), renal disease (≈2.6-fold), anemia (≈2-fold), and dyslipidemia (≈1.2-fold). Conclusions Patients ≥80 years old had higher rates of in-hospital MAE compared with patients aged <80 years old. Female sex and the presence of heart failure, diabetes, renal disease, and anemia were factors associated with in-hospital MAE among both groups.
Respiratory Ammonification of Nitrate Coupled to Anaerobic Oxidation of Elemental Sulfur in Deep-Sea Autotrophic Thermophilic Bacteria (2017)

Respiratory ammonification of nitrate is the microbial process that determines the retention of nitrogen in an ecosystem. To date, sulfur-dependent dissimilatory nitrate reduction to ammonium has been demonstrated only with sulfide as an electron donor. We detected a novel pathway that couples the sulfur and nitrogen cycles. Thermophilic anaerobic bacteria <i>Thermosulfurimonas dismutans</i> and <i>Dissulfuribacter thermophilus</i>, isolated from deep-sea hydrothermal vents, grew autotrophically with elemental sulfur as an electron donor and nitrate as an electron acceptor producing sulfate and ammonium. The genomes of both bacteria contain a gene cluster that encodes a putative nitrate ammonification enzyme system. Nitrate reduction occurs via a Nap-type complex. The reduction of produced nitrite to ammonium does not proceed via the canonical Nrf system because nitrite reductase NrfA is absent in the genomes of both microorganisms. The genome of <i>D. thermophilus</i> encodes a complete sulfate reduction pathway, while the Sox sulfur oxidation system is missing, as shown previously for <i>T. dismutans</i>. Thus, in high-temperature environments, nitrate ammonification with elemental sulfur may represent an unrecognized route of primary biomass production. Moreover, the anaerobic oxidation of sulfur compounds coupled to growth has not previously been demonstrated for the members of <i>Thermodesulfobacteria</i> or <i>Deltaproteobacteria</i>, which were considered exclusively as participants of the reductive branch of the sulfur cycle.
Uncontrolled modulation imaging (2004)

To obtain high dynamic range or hyperspectral images, multiple frames of the same field of view are acquired while the imaging settings are modulated; images are taken at different exposures or through different wavelength bands. A major problem associated with such modulations has been the need for perfect synchronization between image acquisition and modulation control. In the past, this problem has been addressed by using sophisticated servo-control mechanisms. In this work, we show that the process of modulation imaging can be made much simpler by using vision algorithms to automatically relate each acquired frame to its corresponding modulation level. This correspondence is determined solely from the acquired image sequence and does not require measurement or control of the modulation. The image acquisition and the modulation work continuously, in parallel, and independently. We refer to this approach as computational synchronization. It makes the imaging process simple and easy to implement. We have developed a prototype modulation imaging system that uses computational synchronization and used it to acquire high dynamic range and multispectral images.
60-Gb/s Receiver Employing Heterogeneously Integrated Silicon Waveguide Coupled Photodetector (2013)

We demonstrate a digital optical receiver consisting of a heterogeneously integrated silicon waveguide coupled photodetector wire-bonded to a silicon-germanium receiver operating with bit error rate below 10−12 at data rates up to 60 Gb/s.
A new 68Ga-labeled somatostatin analog containing two iodo-amino acids for dual somatostatin receptor subtype 2 and 5 targeting (2020)

[<sup>68</sup>Ga]Ga-DOTA-ST8950 reveals its potential for PET imaging SST2- and SST5-expressing tumors. It compares favorably with the clinically used [<sup>68</sup>Ga]Ga-DOTA-NOC in terms of tumor uptake; however, its uptake in the liver remains a challenge for clinical translation. In addition, this study reveals the essential role of the iodo-substitutions in positions 1 and 3 of [<sup>68</sup>Ga]Ga-DOTA-ST8950 for maintaining affinity to SST2 and SST5, as the de-iodinated [<sup>68</sup>Ga]Ga-DOTA-ST8951 lost affinity for both receptor subtypes.
Truly wearable display comprised of a flexible battery, flexible display panel, and flexible printed circuit (2014)

Abstract In an effort to create a truly flexible and wearable display having a flexible battery as well as a flexible organic light‐emitting diode panel and a flexible printed circuit, a flexible lithium‐ion battery has been developed, and a prototype wrist‐wearable or arm‐wearable display has been fabricated. Owing to improvements in the internal structure and exterior of the lithium‐ion battery, no remarkable changes in charge and discharge curves and the internal state of the electrodes were observed even after conducting a 10,000‐cycle bending test. Therefore, this flexible lithium‐ion battery prototype demonstrated remarkable bending resistance. Thus, we succeeded in fabricating a truly flexible and wearable display comprised of a flexible organic light‐emitting diode panel, a flexible printed circuit, and a flexible battery.
Metabolites in ventricular cerebrospinal fluid detected by proton magnetic resonance spectroscopic imaging (2004)

Normally, ventricular cerebrospinal fluid (CSF) contains low levels of all metabolite signals on proton magnetic resonance spectroscopic imaging (MRSI). We present here three cases (two with seizure disorders, one with a central nervous system lymphoma) who presented with unusually elevated CSF signals on MRSI. Based on chemical shifts and in vitro studies (in one case), the signals were assigned to propan-1,2-diol (PD), acetone, and lactate, respectively. These compounds were either exclusively, or more readily, detected in CSF than in brain. Proton MRSI conveniently screens both brain and CSF for abnormal metabolism simultaneously.
Enzymatic breakage and joining of deoxyribonucleic acid. IV. DNA synthesis in E. coli infected with ligase-negative mutants of phage T4. (1968)

Polynucleotide ligases have been isolated from both uninfected Escherichia coli and from phage T4-infected E. Coli.'-6Both enzymes catalyze the repair of single-strand breaks in bihelical DNA by forming phosphodiester bonds, but they can be distinguished by their cofactor requirements, diphosphopyridine nucleotide (DPN)' 7 and adenosine triphosphate (ATP),4 respectively.Repair of single-strand breaks in DNA may be important in DNA replication, repair, and recombination.The phage T4-induced ligase may participate in one or more of these processes, since gene 30 has been identified as the structural gene for the T4 ligase,8 and mutants defective in this gene have been found to be defective in the synthesis of Studies described in a separate report'3 failed to reveal any defect in the for- mation of recombinant molecules in the absence of a functional T4 ligase.The present report, in conjunction with another paper,'3 describes studies on the synthesis of phage DNA in E. coli infected with ligase-negative mutants of phage T4. Simir studies have been carried out by Sugimoto, Okazaki, and Okazaki,'4 Hosoda,'0' 15 Newman and Hanawalt,16 and Kozinski.'7ExperImental Procedure.-Bacterial srains: E. coli BB is the nonpermissive host oJ the T4 amber mutants; E. coli CR63 is the permissive host.Bacteriophages: T4amH39X (gene 30 amber mutant), T4tsB20 (gene 30 temperature- sensitive mutant), and T4amN82 (gene 44 amber mutant, DNA negative) were used.Media: Either TCG18 or M9 media'9 modified to contain 0.2% casamino acid were used for the growth of bacteria and phage.Adsorption of phage to bacteria was carried out in the "adsorption medium" described by Hershey and Chase.20 Preparation of '2P-phages: T4 phages labeled with 32p were grown in TCG medium, * This investigation was supported by a U.S.
Solving Inverse Problems With Piecewise Linear Estimators: From Gaussian Mixture Models to Structured Sparsity (2011)

A general framework for solving image inverse problems with piecewise linear estimations is introduced in this paper. The approach is based on Gaussian mixture models, which are estimated via a maximum a posteriori expectation-maximization algorithm. A dual mathematical interpretation of the proposed framework with a structured sparse estimation is described, which shows that the resulting piecewise linear estimate stabilizes the estimation when compared with traditional sparse inverse problem techniques. We demonstrate that, in a number of image inverse problems, including interpolation, zooming, and deblurring of narrow kernels, the same simple and computationally efficient algorithm yields results in the same ballpark as that of the state of the art.
Reactivity of Chromium Complexes of a Bis(imino)pyridine Ligand:  Highly Active Ethylene Polymerization Catalysts Carrying the Metal in a Formally Low Oxidation State (2007)

A divalent chromium complex of bis(imino)pyridine, {2,6-[2,6-(i-Pr)2PhNC(CH3)]2(C5H3N)}CrCl2 (1), was prepared with the aim of studying its reactivity with alkylating agents. Upon treatment with MeLi, the metal center was both reduced and alkylated, forming {2,6-[2,6-(i-Pr)2PhNC(CH3)]2(C5H3N)}CrMe(μ-Me)Li(THF)3 (2). Complex 1 is also conveniently reduced with either NaH or metallic sodium to give the new species {2,6-[2,6-(i-Pr)2PhNC(CH3)]2(C5H3N)}CrCl (3). Despite the appearance of the metal center in a rare monovalent oxidation state, the square-planar geometry of the Cr atom suggests that the metal is most likely divalent, with the electron housed in the ligand π* orbital. When it is activated with MAO, complex 3 is a very and even more active catalyst for the polymerization of ethylene than either the −CrCl2 or −CrCl3 derivative of this ligand system and yet produces polymers with similar properties. Subsequent reactivity studies of complex 3 have allowed the isolation of several products. Reaction with either LiCH2Si(CH3)3 or MeLi resulted in deprotonation of one of the methyl groups on the ligand backbone, forming {2-[2,6-(i-Pr)2PhNC(CH3)]-6-[2,6-(i-Pr)2PhNCCH2](C5H3N)}Cr(THF) (4) and {2-[2,6-(i-Pr)2PhNC(CH3)]-6-[2,6-(i-Pr)2PhNCCH2](C5H3N)}Cr(μ-Me)Li(THF)3 (5), respectively. On the other hand, alkylation with AlMe3 allowed the successful preparation of another organochromium species, {2,6-[2,6-(i-Pr)2PhNC(CH3)]2(C5H3N)}CrCH3 (7), along with small amounts of the byproduct {2,6-[2,6-(i-Pr)2PhNC(CH3)]2(C5H3N)}Cr(μ-Cl)2Al(CH3)2 (6). Interestingly, complex 7, which also has the deceiving connectivity of a monovalent species, displays an even greater activity for ethylene polymerization than all of the other species reported herein, again producing a polymer with nearly identical characteristics. Activation with IBAO revealed a deactivation pathway similar to that observed with the FeCl2 system. In this case, the stronger reducing power of IBAO resulted in the usual reduction not only of the ligand backbone but also of the metal center. As a result of the metal reduction, partial transmetalation of the ligand system occurred, with formation of [η4-{2,6-[2,6-(i-Pr)2PhNC(CH3)]2(C5H3N)}Al2(i-Bu)3(μ-Cl)]Cr-(η6-C7H8) (8). By being catalytically inactive, the partly transmetalated 8 suggests that ligand demetalation is a possible catalyst deactivation pathway.
Cerebral MR angiography with multiple overlapping thin slab acquisition. Part I. Quantitative analysis of vessel visibility. (1991)

Multiple overlapping thin slab acquisition (MOTSA) is a novel time-of-flight magnetic resonance (MR) angiography technique that combines many of the advantages of multiple-section two-dimensional and direct three-dimensional volume methods. To rigorously evaluate the vascular detail that can be obtained with MOTSA MR angiography, the authors have quantified the frequency of visibility of intracranial arterial segments and have correlated their visibility with vessel size as measured at cut-film angiography and with the position and orientation of the arterial segment. Intracranial arterial segments at least 0.9 mm in diameter (the voxel dimension used in the current application) are consistently visualized with the MOTSA technique. The fraction of small vessels visualized is improved when voxel dimensions are reduced. Visibility is noticeably better in the primary images compared with the maximum-intensity-projection (MIP) images. Top-of-slab saturation and resulting decreased vessel intensity occurred infrequently and did not result in marked image degradation in the axial images.
The Parkinson’s disease-linked protein TMEM230 is required for Rab8a-mediated secretory vesicle trafficking and retromer trafficking (2016)

TMEM230 is a newly identified Parkinson's disease (PD) gene encoding a transmembrane protein whose cellular and pathogenic roles remain largely unknown. Here, we demonstrate that loss of TMEM230 disrupts retromer cargo CI-M6PR (cation-independent mannose 6-phosphate receptor) trafficking and autophagic cargo degradation rates. TMEM230 depletion further inhibits extracellular secretion of the autophagic cargo p62 and immature lysosomal hydrolases in Golgi-derived vesicles leading to their intracellular accumulation, and is specifically mediated by loss of the small GTPase Rab8a. Importantly, PD-linked TMEM230 variants also induce retromer mislocalization, defective cargo trafficking, and impaired autophagy. Finally, we show that knockdown of another PD gene, LRRK2, which phosphorylates Rab8a, similarly impairs retromer trafficking, secretory autophagy and Golgi-derived vesicle secretion, thus demonstrating converging roles of two PD genes TMEM230 and LRRK2 on Rab8a function, and suggesting that retromer and secretory dysfunction play an important role in PD pathogenesis.
A Pathogen Receptor on Natural Killer Cells (2002)

Targeting NKG2A to elucidate natural killer cell ontogenesis and to develop novel immune-therapeutic strategies in cancer therapy,
Supplementary Figures 1-6 from Androgen Receptor Signaling in Circulating Tumor Cells as a Marker of Hormonally Responsive Prostate Cancer (2023)

&lt;p&gt;PDF file - 3.8MB, Supplemental Figures. (S1) AR transcriptional signature derivation and validation. (S2) Time course for immunofluorescence assay for AR signaling. (S3) Time course for multiparameter single cell immunofluorescence assay for AR signaling applied to VCaP prostate cancer cells. (S4) Immunofluorescence images of LNCaP cells treated with R1881 or bicalutamide and captured on HB CTC-chip. (S5) Enumeration of CTCs in healthy donor controls, male patients without cancer, and metastatic prostate cancer patients. (S6) Kaplan-Meier survival curves for overall survival in patients treated with abiraterone acetate according to increase in % AR-on CTCs and PSA response.&lt;/p&gt;
Mechanism of Inactivation of γ-Aminobutyric Acid Aminotransferase by (S)-4-Amino-4,5-dihydro-2-thiophenecarboxylic Acid (1999)

(S)-4-Amino-4,5-dihydro-2-thiophenecarboxylic acid ((S)-6) was previously synthesized (Adams, J. L.; Chen, T. M.; Metcalf, B. W. J. Org. Chem. 1985, 50, 2730−2736.) as a heterocyclic mimic of the natural product gabaculine (5-amino-1,3-cyclohexadienylcarboxylic acid), a mechanism-based inactivator of γ-aminobutyric acid aminotransferase (GABA-AT) (Rando, R. R. Biochemistry 1977, 16, 4604). Inactivation of GABA-AT by (S)-6 is time-dependent and protected by substrate. Two methods were utilized to demonstrate that, in addition to inactivation, about 0.7 equiv per inactivation event undergoes transamination. Inactivation results from the reaction of (S)-6 with the pyridoxal 5‘-phosphate (PLP) cofactor. The adduct was isolated and characterized by ultraviolet−visible spectroscopy, electrospray mass spectrometry, and tandem mass spectrometry. All of the results support a structure (11) that derives from the predicted aromatization inactivation mechanism (Scheme 2) originally proposed by Metcalf and co-workers for this compound. This is only the third example, besides gabaculine and l-cycloserine, of an inactivator of a PLP-dependent enzyme that acts via an aromatization mechanism.