gebhart/patrepeval huggingface dataset:
Broad‐host range expression vectors containing manipulated meta‐cleavage pathway regulatory elements of the TOL plasmid (1988)

The construction of pERD20 and pERD21, two broad-host range expression vectors, is described. The vectors contain the Pm promoter of the meta-cleavage pathway operon of the TOL plasmid pWWO; this promoter is present within a polylinker which provides a number of downstream cloning sites close to the transcription initiation site. Transcription from the Pm promoter in these vectors is controlled not by the natural positive regulator of Pm, the Xy1S protein, but by an Xy1S mutant analogue, Xy1S2tr6, which inhibits an altered effector specificity and can mediate a 3-8-fold higher level of transcription than can Xy1S in a wide range of temperatures. Controlled expression of cloned genes can be achieved in a broad spectrum of Gram negative bacteria grown at a wide range of temperatures.
Solvent‐Free Synthesis of Silicoaluminophosphate Zeolites (2013)

Solvents, who needs them? Silicoaluminophosphate zeolites with various frameworks have been successfully synthesized by a solvent-free route. Catalytic tests for the conversion of methanol to olefins show that thus obtained SAPO-34 (see picture) exhibited catalytic performance comparable to that of zeolites made by the conventional hydrothermal route.
Impact of stress on various circuit characteristics in 65nm PDSOI technology (2007)

Logic performance is improved by creating more stress in the channel in advanced CMOS technologies. Impact of stress on different circuit blocks in a microprocessor chip has not been studied in detail. This paper presents a comprehensive study on the effects of stress and the corresponding process steps on various circuit characteristics. Analog behavior, hysteresis and noise properties are investigated to understand the effect of stress on them. These characteristics play important roles in determining the performances of analog/phy, I/O and PLL blocks respectively. It is shown that the type of process steps used for stress optimization can significantly alter the performance of various circuits.
Development of an Automated Drug Delivery System for an Ultra-Short-acting β-Blocker, Landiolol, to Stably Reduce Myocardial Oxygen Consumption without Inducing Circulatory Collapse in a Canine Heart Failure Model (2020)

<title>Abstract</title> <bold>Background:</bold> Beta-blockers are well known to reduce myocardial oxygen consumption (MVO<sub>2</sub>) and improve the prognosis of heart failure (HF) patients. Although the use of β-blockers in the acute phase of HF can be expected to be beneficial, the negative chronotropic and inotropic effects limit their use due to the risk of circulatory collapse (cardiogenic shock, and/or pulmonary congestion). A safe method to administer β-blockers in the acute phase of HF is in great need. In this study, we developed an automated drug delivery system that controls the infusion of landiolol, an ultra-short-acting β-blocker, while preventing circulatory collapse. <bold>Method:</bold> We designed a system that simultaneously regulates cardiac function and volume status to control haemodynamics. The system monitors arterial pressure (AP), left atrial pressure (P<sub>LA</sub>), right atrial pressure, and cardiac output. Using negative feedback of haemodynamics, the system controls mean AP and mean P<sub>LA</sub> by administering landiolol, dextran, and furosemide. We applied the system for 60 min to 5 mongrel dogs with rapid pacing-induced HF, and assessed haemodynamics, MVO<sub>2</sub> and lactate.<bold>Results:</bold> In all dogs, the system successfully adjusted delivery of the drugs resulting in accurate control of mean AP and mean P<sub>LA</sub>. From 15 to 60 min after the system was activated, median of absolute performance error (index of precision of control) was small for mean AP (median [interquartile range], 2.5 [2.1 – 3.7] %) and mean P<sub>LA</sub> (4.1 [1.8 – 6.2] %). Although the system decreased mean AP compared to baseline, mean and systolic AP were maintained not lower than 70 and 100 mmHg, respectively, and lactate did not increase. Furthermore, the system significantly decreased P<sub>LA</sub> and MVO<sub>2</sub> (3.6 [3.3 – 4.0] to 2.7 [2.5 – 3.3] ml·min<sup>-1</sup>·100 g left ventricular weight<sup>-1</sup>) compared to baseline. Consequently, the automated drug delivery system successfully reduced MVO<sub>2</sub> without inducing circulatory collapse.<bold>Conclusion:</bold> We developed an automated landiolol delivery system that achieved safe administration of landiolol in a canine model of acute HF. The system controlled AP and P<sub>LA</sub> accurately and stably, and reduced MVO<sub>2</sub>. With further development for clinical application, the automated drug delivery system may be the key tool to improve management of patients with HF.
Electron diffraction tomography and X-ray powder diffraction on photoredox catalyst PDI (2019)

The crystal structure of photoredox catalyst PDI-iPr was determined by a combination of electron diffraction tomography and XRPD.
Pulsed-power-handling capability of optical fibers (1986)

Crucial issues are presented for quantifying the damage and power handling characteristics of optical fibers for laser beam delivery and sensing as well as communications applications. In addition to the intrinsic surface and bulk damage threshold of a given fiber material, there are other factors to consider in efforts to quantify the onset of damage and/or undesirable effects. Included are nongaussian and nonuniform light distribution in the fiber, the nature of the core/cladding interface, and nonlinear phenomena (e.g., self-focusing). These aspects are illustrated by experiments. Theoretical modeling is under way to help elucidate the role of these factors.
Passwords and the evolution of imperfect authentication (2015)

Theory on passwords has lagged practice, where large providers use back-end smarts to survive with imperfect technology.
Electrical currents and liquid flow rates in micro-reactors (2001)

For micro-reactor devices in which liquids are pumped by electro-osmotic flow (EOF), in situ monitoring of the electrical currents in the channel networks provides a valuable diagnostic tool. We demonstrate here that the voltage-current characteristics of a micro-reactor channel network can be accurately modelled using measurements of the full 3-D geometry of the channel network, the liquid conductivity and the channel wall-liquid surface conductivity. It is shown that surface conductivity provides a significant contribution to the overall measured electrical currents in channel networks for which the ratio of surface area to volume is high. Following correction for surface conductivity, the electrical currents are proportional to the liquid volumetric flow rates measured in the different branches of the channel network. The constant of proportionality is related to the zeta potential of the channel wall-liquid surface. Measurements of the variation of electrical currents and volumetric flow rates as a function of the applied voltages allows the determination of the surface conductivity and zeta potential within the micro-reactor which enables the prediction of the voltages required to produce the desired flow rates in any channel section. In situ logging of the electrical currents, incorporated within the control system, allows continuous monitoring of the liquid flow rates during micro-reactor operation.
Mechanism of Temperature Dependence of Piezoelectric Properties for Pb(Zr, Ti)O3 (1993)

Temperature dependence of piezoelectric properties was measured for a tetragonal and a rhombohedral Pb(Zr, Ti)O 3 (denoted as t -PZT and r -PZT, respectively). A least squares fitting of complex admittance data was employed in order to precisely determine various electromechanical constants. It was confirmed that the piezoelectric constant d 31 of the t -PZT was thermally stable while that of the r -PZT largely increased with temperature. It was clarified that the elastic compliance s 11 E and the electromechanical coupling factor k 31 decreased and the dielectric constant ε 33 T increased with temperature in the t -PZT, giving rise to the thermally stable behavior of d 31 . In the case of the r -PZT, the increase in ε 33 T dominated the temperature dependence of the d 31 . From the XRD analysis, it seemed that the increase in ε 33 T of the r -PZT originated from a large thermal expansion of the unit cell volume.
RETROSPECTIVE: Corona: System Implications of Emerging Nanophotonic Technology (2023)

The 2008 Corona effort was inspired by a pressing need for more of everything, as demanded by the salient problems of the day. Dennard scaling was no longer in effect. A lot of computer architecture research was in the doldrums. Papers often showed incremental subsystem performance improvements, but at incommensurate cost and complexity. The many-core era was moving rapidly, and the approach with many simpler cores was at odds with the better and more complex subsystem publications of the day. Core counts were doubling every 18 months, while per-pin bandwidth was expected to double, at best, over the next decade. Memory bandwidth and capacity had to increase to keep pace with ever more powerful multi-core processors. With increasing core counts per die, inter-core communication bandwidth and latency became more important. At the same time, the area and power of electrical networks-on-chip were increasingly problematic: To be reliably received, any signal that traverses a wire spanning a full reticle-sized die would need significant equalization, re-timing, and multiple clock cycles. This additional time, area, and power was the crux of the concern, and things looked to get worse in the future. Silicon nanophotonics was of particular interest and seemed to be improving rapidly. This led us to consider taking advantage of 3D packaging, where one die in the 3D stack would be a photonic network layer. Our focus was on a system that could be built about a decade out. Thus, we tried to predict how the technologies and the system performance requirements would converge in about 2018. Corona was the result this exercise; now, 15 years later, it's interesting to look back at the effort.
Reliability projecting for ReRAM based on stochastic differential equation (2016)

An analytic formula based on stochastic differential equation is successfully developed to describe intrinsic ReRAM variation. The formula is useful to predict scaled ReRAM memory window after retention, verified by testing 40 nm 2Mb memory array.
Supplementary Tables S1-S2, Supplementary Figures S1-S5 from PDCD4 Is an Androgen-Repressed Tumor Suppressor that Regulates Prostate Cancer Growth and Castration Resistance (2023)

&lt;p&gt;Supplementary Table S1: siRNA and shRNA Vectors Supplementary Table S2: The sequence of primers used in this study Supplementary Figure S1. miR-21 suppresses PDCD4 expression in normal prostate epithelial cells. Supplementary Figure S2. Androgen signaling and miR-21 suppresses PDCD4 mRNA expression. Supplementary Figure S3. Transient PDCD4 over-expression and PDCD4 knockdown. Supplementary Figure S4. Transient PDCD4-overexpression induces apoptosis of LAPC4, but not LNCaP, cells. Supplementary Figure S5. PDCD4 expression regulates cell proliferation and apoptosis.&lt;/p&gt;
Supplementary document for Ultrabroadband Nonlinear Optics in Nanophotonic Periodically Poled Lithium Niobate Waveguides - 4166195.pdf (2020)

Supplemental Document - Derivation of Nonlinear Coupling
A Noncubic Triply Periodic Network Morphology in Poly(isoprene-b-styrene-b-ethylene oxide) Triblock Copolymers (2002)

We present characterization data for linear poly(isoprene-b-styrene-b-ethylene oxide) (PI−PS−PEO) triblock copolymers containing equal volume fractions of PI and PS (fPI = fPS) and varying amounts of PEO, 0 ≤ fPEO ≤ 1/3. Detailed characterization by TEM, SAXS, dynamic mechanical spectroscopy, static birefringence, and DSC indicates three distinct morphologies: two-domain lamellae, a three-domain noncubic triply periodic network structure, and three-domain lamellae with increasing PEO content. This phase behavior is in striking contrast with the homologous PS−PI−PEO triblock phase diagram, reported earlier, which contains four different morphologies between the limiting two- and three-domain lamellae phases. Preliminary analysis suggests the noncubic network structure contains elementary units of 3-fold coordination, yet fundamentally distinct from the cubic core−shell gyroid topology previously demonstrated in PS−PI−PEO. This tentatively assigned model is a structural hybrid of the gyroid and perforated lamellar morphologies. These results provide quantitative evidence regarding the effects of block sequencing on the phase behavior of ABC triblock copolymers near the order−disorder transition.
Gradient-Based Fingerprinting for Indoor Localization and Tracking (2015)

Of the different branches of indoor localization research, WiFi fingerprinting has drawn significant attention over the past decade. These localization systems function by comparing WiFi received signal strength indicator (RSSI) and a pre-established location-specific fingerprint map. However, due to the time-variant wireless signal strength, the RSSI fingerprint map needs to be calibrated periodically, incurring high labor and time costs. In addition, biased RSSI measurements across devices along with transmission power control techniques of WiFi routers further undermine the fidelity of existing fingerprint-based localization systems. To remedy these problems, we propose GradIent FingerprinTing (GIFT) which leverages a more stable RSSI gradient. GIFT first builds a gradient-based fingerprint map (Gmap) by comparing absolute RSSI values at nearby positions, and then runs an online extended particle filter (EPF) to localize the user/device. By incorporating Gmap, GIFT is more adaptive to the time-variant RSSI in indoor environments, thus effectively reducing the overhead of fingerprint map calibration. We implemented GIFT on Android smartphones and tablets, and conducted extensive experiments in a five-story campus building. GIFT is shown to achieve an 80 percentile accuracy of 5.6 m with dynamic WiFi signals.
Guardrail Flare Rates (2007)

The potential to increase suggested flare rates for strong post W-beam guardrail systems, and thus reduce guardrail installation lengths, is investigated. This reduction in length would result in decreased guardrail construction and maintenance costs and reduce impact frequency with only modest increases in impact severity. Computer simulation and five full-scale crash tests were completed to evaluate increased flare rates up to, and including, 5:1 on the Midwest Guardrail System (MGS). Impact severities during testing were found to be greater than intended, yet the MGS passed all NCHRP 350 requirements. Hence, flaring the MGS guardrail as much as 5:1 will still provide acceptable safety performance for the full range of passenger vehicles. Increasing guardrail flare rates will reduce the overall number of guardrail crashes without significantly increasing risks of injury or fatality during the remaining crashes. Therefore, it is recommended that whenever guardrail is outside of the shy line for adjacent traffic and the roadside terrain is sufficiently flat, flare rates increase to as high as 5:1.
Recent advances in catalytic antibodies (1991)

Abstract Recently the biological machinery of the immune system has been exploited with the aid of mechanistic chemistry to produce catalytic antibodies. Because antibodies can be generated that selectively bind almost any molecule of interest, this new technology offers the potential to tailor-make highly selective catalysts for applications in biology, chemistry and medicine. In addition, catalytic antibodies provide fundamental insight into important aspects of biological catalysis, including the importance of transitionstate stabilization, proximity effects, general acid and base catalysts, electrophilic and nucleophilic catalysis, and strain.
REVERSIBLE, THERMOTROPIC ALTERATION OF NUCLEAR MEMBRANE STRUCTURE AND NUCLEOCYTOPLASMIC RNA TRANSPORT IN TETRAHYMENA (1974)

We examine the effect of cooling upon the freeze-etch ultrastructure of nuclear membranes, as well as upon nucleocytoplasmic RNA transport in the unicellular eukaryote Tetrahymena pyriformis. Chilling produces smooth, particle-free areas on both faces of the two freeze-fractured macronuclear membranes. Upon return to optimum growth temperature the membrane-associated particles revert to their normal uniform distribution and the smooth areas disappear. Chilling lowers the incorporation of [(14)C]uridine into whole cells and their cytoplasmic RNA. Cooling from the optimum growth temperature of 28 degrees to 18 degrees C (or above) decreases [(14)C]uridine incorporation into cells more than into their cytoplasmic RNA; chilling to below 18 degrees C but above 10 degrees C causes the reverse. [(14)C]Uridine incorporation into whole cells and their cytoplasmic RNA reflects overall RNA synthesis and nucleocytoplasmic RNA transport, respectively. RNA transport decreases strongly between 20 degrees and 16 degrees C, which is also the temperature range where morphologically detectable nuclear membrane transitions occur. This suggests that the nuclear envelope limits the rate of nucleocytoplasmic RNA transport at low temperatures. We hypothesize that a thermotropic lipid phase transition switches nuclear pore complexes from an "open" to a "closed" state with respect to nucleocytoplasmic RNA transport.
A new DNA marker, D3S733, identifies a HindlII polymorphism on chromosome 3p (1991)

XLIB17-78 (D3S733) is a 5.0 kb EcoRI insert isolated from a Charon 21 A human chromosome 3 library. Polymorphism: HindIII digestion of genomic DNA and hybridization with the probe detects a two-allele polymorphism: 12.0 kb (H1) and 7.5 kb (H2). Frequency: Estimated from 120 unrelated Caucasians. HI: 0.2 H2: 0.8 Frequency of Heterozygosity: 0.32. Observed frequency of heterozygosity = 0.37. Not Polymorphic For: BamHI, BglII, Dral, EcoRI, Hinfi, MspI, PvuII and TaqI.
Periodic Cation Segregation in Cs0.44[Nb2.54W2.46O14] Quantified by High-Resolution Scanning Transmission Electron Microscopy (2014)

The atomic structure of Cs0.44[Nb2.54W2.46O14] closely resembles the structure of the most active catalyst for the synthesis of acrylic acid, the M1 phase of Mo10V2(4+)Nb2TeO42-x. Consistently with observations made for the latter compound, the high-angle electron scattering signal recorded by scanning transmission electron microscopy shows a significant intensity variation, which repeats periodically with the projected crystallographic unit cell. The occupation factors for the individual mixed Nb/W atomic columns are extracted from the observed intensity variations. For this purpose, experimental images and simulated images are compared on an identical intensity scale, which enables a quantification of the cation distribution. According to our analysis specific sites possess low tungsten concentrations of 25%, whereas other sites have tungsten concentrations above 70%. These findings allow us to refine the existing structure model of the target compound, which has until now described a uniform distribution of the niobium and tungsten atoms in the unit cell, showing that the similarity between Cs0.44[Nb2.54W2.46O14] and the related catalytic compounds also extends to the level of the cation segregation.
Abstract 175: A Novel Orally-Active Small-Molecule Antagonist of the Platelet Protease-Activated Receptor-4, BMS-986120, Inhibits Arterial Thrombosis With Limited Impact on Hemostasis in Cynomolgus Monkeys (2016)

Introduction: BMS-986120 (BMS) is a novel orally-active antagonist of protease-activated receptor-4 (PAR4), a human platelet thrombin receptor, and is in phase I clinical trial. The antithrombotic potential of BMS was studied in models of electrically-mediated carotid artery thrombosis and bleeding time (BT) in cynomolgus monkeys, which have platelet thrombin receptors similar to human. Methods: Individual anesthetized monkeys were given orally of BMS (0.2, 0.5,1 mg/kg) or vehicle (n=8/group) 2 hour before a combination of thrombosis, BT and ex vivo biomarker experiments. Aspirin alone (ASA, 4 mg/kg/h IV) or in combination with BMS (0.5, 1 mg/kg) was also studied (n=8/group). Thrombus weight (TW) reduction, BT increase over vehicle in kidney (KBT) and mesenteric artery (MBT), and platelet aggregation (PA) inhibition were determined. Peak PA responses to activation peptides selective for PAR4 (PAR4-AP, 12.5 μM) and PAR1 (PAR1-AP, 18 μM), ADP (20 μM), and collagen (5 μg/ml) were determined by whole blood aggregometry. Results: BMS comparably inhibited PA induced by PAR4-AP in human and monkey blood in vitro (IC 50 of 9.5±2.7 and 2.1±0.4 nM, respectively). In monkeys, BMS produced parallel rightward shifts in the log PA dose response to PAR4-AP without affecting maximum response, suggesting surmountable antagonism. BMS (1 mg/kg) did not inhibit PA induced by PAR1-AP, ADP and collagen, supporting selectivity. BMS (0.2, 0.5, 1 mg/kg) reduced TW by 35±5, 49±4, and 83±4%, respectively. Maximum KBT and MBT increases were only 2.2-fold and 1.8-fold, respectively. A maximum antiplatelet dose of ASA (4 mg/kg/h, n=8) slightly reduced TW by 12±2% and increased KBT and MBT by 2.2- and 2.7-fold, respectively. Co-administration of ASA and BMS (0.5 or 1 mg/kg) reduced TW by 54±3 and 95±2%, increased KBT by 3.1- and 3.6-fold, and increased MBT by 2.6- and 3.3-fold, respectively (n=8/group). In companion monkey studies, clopidogrel (0.3 mg/kg/day, n=6) alone reduced TW by 49±6%, but increased KBT and MBT by 7.3- and 8.1-fold, respectively. Conclusion: In monkeys, BMS alone or combined with ASA prevents occlusive carotid artery thrombosis with limited impact on BT, demonstrating a wider therapeutic window than the standard of care antiplatelet drugs, aspirin and clopidogrel.
Pulse-shaping versus digital backpropagation in 224Gbit/s PDM-16QAM transmission (2011)

We investigate the transmission performance of 224Gbit/s polarization-division-multiplexed 16-state quadrature amplitude modulation (PDM-16QAM) for systems employing standard single mode fiber (SSMF) and erbium doped fiber amplifiers (EDFAs). We consider the effectiveness of return-to-zero (RZ) data pulses with varying duty cycles and digital backpropagation (DBP) in reducing nonlinear distortion in wavelength-division- multiplexed (WDM) links with 3, 5, 7 and 9 channels. Similar improvement in transmission reach of 18-25% was achieved either by pulse-carving at the transmitter or by DBP, yielding maximum transmission distances of up to 1760km for RZ-pulse-shapes and 1280km for NRZ.
Identification of irregular motion in automotive navigation systems using novelty detection (2016)

Automated display testing for visual unpleasant and erroneous navigation sequences is an important step to preserve a high quality standard for premium vehicle manufacturers. This paper presents a novel error detection algorithm for navigation sequences based on novelty detection on motion parameters obtained from real world navigation sequences. Motion parameters are accumulated through key point matching using BRISK and subsequent homography calculation. With these parameters one is able to describe the motion between two successive frames. Combinations of translational and rotational components allow the novelty detection algorithm to predict outliers. These outliers either show positioning errors or abnormal motion behaviour which are both unacceptable for high quality. Experimental results demonstrate that this algorithm works significantly better than state of the art, where one has to know errors before analysing the data set in order to determine thresholds for particular errors. The gains in precision and recall are 49.67% and 6.06% respectively, the accuracy is 1.37% higher compared to optimized threshold results.