gebhart/patrepeval huggingface dataset:
The [4Fe–4S] cluster of quinolinate synthase from Escherichia coli: Investigation of cluster ligands (2008)

Nicotinamide adenine dinucleotide (NAD) derives from quinolinic acid which is synthesized in Escherichia coli from l-aspartate and dihydroxyacetone phosphate through the concerted action of l-aspartate oxidase and the [4Fe-4S] quinolinate synthase (NadA). Here, we addressed the question of the identity of the cluster ligands. We performed in vivo complementation experiments as well as enzymatic, spectroscopic and structural in vitro studies using wild-type vs. Cys-to-Ala mutated NadA proteins. These studies reveal that only three cysteine residues, the conserved Cys113, Cys200 and Cys297, are ligands of the cluster. This result is in contrast to the previous proposal that pointed the three cysteines of the C(291)XXC(294)XXC(297) motif. Interestingly, we demonstrated that Cys291 and Cys294 form a disulfide bridge and are important for activity.
Phase 2 trial of immunotherapy in tumors with CDK12 inactivation (IMPACT): Results from cohort A of patients (pts) with metastatic castration resistant prostate cancer (mCRPC) receiving dual immune checkpoint inhibition (ICI). (2022)

103 Background: Prostate cancer with CDK12 inactivation represents a distinct subtype in mCRPC, tumors are characterized by excessive tandem duplications, genomic instability, gene fusion-caused putative neoantigens and increased tumor T cell infiltration. Retrospective experiences with ICI in CDK12 inactivation CRPC pts reported PSA and radiographic responses. We conducted a prospective multi-site clinical trial of ipilimumab and nivolumab in CDK12 inactivation or mutated cancers. Herein, we report our findings in the completed cohort A of men with mCPRC. Methods: Eligible pts had mCRPC (ongoing androgen deprivation therapy with serum testosterone £ 50 ng/dL) and putative CDK12 inactivation of function aberrations on any commercial or institutional CLIA/CAP approved next generation sequencing assay. Archival tumor tissue was requested for correlative biomarker analysis. Pts received nivolumab 3 mg/kg IV and ipilimumab 1 mg/kg IV q3 weeks for up to 4 cycles, followed by maintenance nivolumab at 480 mg IV q4 weeks until disease progression, intolerable toxicity, or consent withdrawal. The primary endpoint was PSA response, defined as a greater than or equal to 50% decline in PSA from baseline. Secondary endpoints included safety/toxicity, secondary efficacy measures including QoL and overall survival. Exploratory objectives included baseline tumor whole exome analysis and changes in circulating immune profiles with therapy. Results: As of data cut-off in Aug 2021, 28 mCRPC pts enrolled in Cohort A; median ECOG PS was 1 (0-2 range), 22/28 had Gleason 8-10 cancer, mean baseline PSA at study entry was 231 ng/dL, all pts had received ≥1 prior oral androgen signaling inhibitor and ≥1 cytotoxic chemotherapy. Unconfirmed PSA ≥30% decline was seen in 6/28 pts (21.4%) and PSA ≥50% decline in 4/28 pts (14.2%). Grade ≥3 possible/probable/definite adverse events were noted in 7/28 (25%) and SAEs in 10/28 pts (35.7%). Six pts (21.4%) experienced a rapid PSA increase by ≥ 10-fold over baseline. Conclusions: Combination immunotherapy was reasonably tolerated in this heavily pre-treated population and was associated with unconfirmed PSA responses in a subset of pts. Ongoing correlative analyses could explain responses mechanistically. Enrollment in Cohort B of non-prostate cancers and Cohort C of nivolumab monotherapy in prostate cancer are still ongoing. Clinical trial information: NCT03570619.
Syndecan‐1 expression is upregulated in degenerating articular cartilage in a transgenic mouse model for osteoarthritis (2005)

The present temporospatial expression data on upregulation of syndecan-1 in articular cartilage during early stages of cartilage degeneration suggest that this molecule is involved in the attempted repair of cartilage fibrillations. Combined with the known role of syndecan-1 during skeletal development and wound healing, this interesting finding warrants further validation.
Fluid Characterisation for Gas Injection Study using Equilibrium Contact Mixing (2002)

Abstract This paper describes the development of an equation of state (EOS) for a gas injection simulation study of a compositionally-grading near-critical oil reservoir. As the number of initial oil sample was limited and no gas-cap gas compositional data was available, the equilibrium contact mixing (ECM) test was used to (a) help tune the EOS to nearcritical phase equilibrium existing at the near-critical gas-oil contact, and (b) to estimate the initial gas-cap PVT properties. Available standard depletion type PVT data, multi-contact swelling test data, slim tube test data and ECM data were used in the development of the full-fluid "detailed" EOS using 16 components. Characterisation of the C7+ fraction together with regression of EOS parameters was carried out to tune the fluid model to match all available measured PVT data. Isothermal compositional gradient calculations were performed for all available samples and then a sample was selected to best represent the most important measured properties such as saturation pressure, saturated density and the compositional gradients in the reservoir. Equilibrium contact mixing data provide key gas-cap gas properties and vapour/liquid phase equilibria data for developing the EOS, also important for studying near-miscible gas injection processes.
A “zig-zag” naphthodithiophene core for increased efficiency in solution-processed small molecule solar cells (2012)

A solution-processed small molecule utilizing a novel 5,10-bis((2-ethylhexyl)oxy)-naphtho[2,3-b:6,7-b0]dithiophene [corrected] "zig-zag" core (zNDT) exhibits high hole mobility, upshifted frontier MO energies, and enhanced photovoltaic cell short-circuit currents, fill-factors, and power conversion efficiencies (4.7%) versus the linear NDT isomer.
PRUNING DATASETS IN DISCRIMINANT ANALYSIS: A DTI STUDY TO SCHIZOPHRENIA (2007)

A comparative study is commonly performed by means of pre-defined or expert selected region of interest (ROI)-analysis or voxel based analysis (VBA). In contrast to these methods, correlations within the data can be modeled by using principal component analysis (PCA) and linear discriminant analysis (LDA). The mapping computed by PCA/LDA is displayed to identify the discriminative regions. A technique called 'pruning' is introduced to iteratively discard misclassified subjects from the cohort. These subjects reside in the region in feature space where the classes are overlapping. As the exact cause of this overlapping is unknown, it is preferable to base the mapping merely on representative prototypes, residing in the nonoverlapping parts of the feature space. After pruning the PCA/LDA mapping, a more pronounced decrease in FA in larger parts of the corpus callosum was observed, compared to conventional VBA
Supplementary Figure 2 from Inactivation of Junctional Adhesion Molecule-A Enhances Antitumoral Immune Response by Promoting Dendritic Cell and T Lymphocyte Infiltration (2023)

Supplementary Figure 2 from Inactivation of Junctional Adhesion Molecule-A Enhances Antitumoral Immune Response by Promoting Dendritic Cell and T Lymphocyte Infiltration
Induction of expression of LAT1, an essential amino acid transporter, in activated T cells (P1182) (2013)

Abstract Activation of T cell accompanies remarkable enhancement of metabolism. Sufficient and continuous supply of nutrient such as amino acid is thus considerable importance to support immune reaction in T cell. However, the molecular mechanism of efficient incorporation of amino acid into activated T cell has not been determined. We characterized LAT1 as an essential amino acid transporter which is highly expressed in activated T cell. TCR stimulation and co-stimulation of human T cell triggered the dramatic induction of LAT1, although neither TCR stimulation nor co-stimulation alone did not affect the LAT1 expression. The promotion of LAT1 expression in activated T cell was prohibited by inhibitor of NF-kB and AP-1. These results indicate that LAT1 expression is induced by full activation of T cell and LAT1 could work as a potential transporter for essential amino acid in activated T cell. Our result that NF-kB and AP-1 are essential for LAT1 expression suggests that nutrient supply system and immune reaction system are included in one program and those systems are activated by the same molecular machinery in T cell.
New approaches to high-capacity multiple-antenna wireless (2002)

Summary form only given. Under Rayleigh flat-fading conditions, it is well-known by now that the capacity of a multiple-antenna wireless link increases linearly with the smaller of the number of transmit or receive antennas, with no increase in bandwidth or transmitted power. This phenomenon was verified experimentally with a scheme called BLAST (Bell Labs Layered Space Time). BLAST requires that the receiver learn the matrix of propagation coefficients. Our current research goal is to realize a high throughput in mobile environments where it may not be feasible to estimate the propagation matrix. A new signalling scheme, unitary space-time modulation, is nearly ideal for this task. We have developed some effective ways to design constellations of these signals. Where a user has extended access to the channel, a variation called differential unitary space-time modulation can be particularly convenient. Moreover space-time signals can function as their own channel codes, with spatial diversity replacing temporal diversity, an effect that we call space-time autocoding. This effect, which may obviate the conventional Shannon requirement to perform channel coding over many independent fades, can benefit differential unitary space-time modulation, as well as schemes where a user has access to the channel for only a short period. For example, within a single coherence interval of 16 symbols, for 7 transmit antennas, 4 receive antennas, and an 18 dB expected SNR, a total of 80 bits (rate 5) can theoretically be transmitted with a block probability of error less than 10/sup -9/, all without any training or knowledge of the propagation matrix.
An Auxiliary Sensor Processor to Provide Real Time Fuel Delivery Feedback for a Microprocessor Based Diesel Engine Controller (1987)

&lt;div class="htmlview paragraph"&gt;The design of a dedicated microprocessor based data acquisition and analysis system is described, as applied in a dual processor diesel engine control system. This auxiliary processor performs the task of sampling available engine sensors and analyzing the data to extract information valuable as feedback to the main controller. Large quantities of data may be taken and analyzed in real time to provide support for more sophisticated and precise engine control strategies, without overloading the main control processor.&lt;/div&gt; &lt;div class="htmlview paragraph"&gt;Specialized hardware and software features are highlighted, which permit rapid data acquisition and analysis, and efficient interprocessor communications. Design issues associated with distributed processing architectures for engine control computers are discussed.&lt;/div&gt; &lt;div class="htmlview paragraph"&gt;A technique for determination of the actual injected fuel quantity from real-time analysis of the injector needle lift signal is suggested as a potential application for the auxiliary processor. This application makes possible true closed-loop fuel quantity control, with the potential for improvements in engine emissions and efficiency. Other possible applications are discussed in the context of more sophisticated control strategies.&lt;/div&gt;
Expression of the T-Cell–Specific Tyrosine Kinase Lck in Normal B-1 Cells and in Chronic Lymphocytic Leukemia B Cells (1998)

Src family kinases play a key role in mitogenesis. The exquisitely tissue-specific distribution of different Src family members suggests that a fine tuning of their expression might be a key prerequisite for cell homeostasis. We tested B cells from patients affected by B-cell chronic lymphocytic leukemia (B-CLL) for expression of Src family kinases. The T-cell-specific tyrosine kinase Lck was found to be expressed at significant levels in CLL B-cells. This finding could be accounted for either by ectopic expression of Lck in B-CLL or by specific expression of this kinase in normal B-1 cells, which are believed to be the normal counterpart of CLL B cells. To answer this question B cells from different sources, characterized by a different size of the B-1 subpopulation, were tested for Lck expression. The results show that Lck expression is a feature of CD5(+), B-1 cells, suggesting a potential role for Lck in the self-renewal capacity of this B-cell subpopulation and supporting the notion that B-1 cells are the subset undergoing oncogenic transformation in B-CLL. Furthermore, we show that the CD5(-), B-2 subpopulation, while normally lacking Lck expression, acquires the capacity to express Lck ectopically upon transformation by EBV.
Gas Chromatography and Selective Mass Detection (2008)

No AccessPlastics Testing and CharacterizationMar 2008Gas Chromatography and Selective Mass DetectionAlberto Naranjo, María del Pilar Noriega E., Tim A. Osswald, Alejandro Roldán-Alzate, Juan Diego SierraAlberto Naranjo, María del Pilar Noriega E., Tim A. Osswald, Alejandro Roldán-Alzate, Juan Diego Sierrahttps://doi.org/10.3139/9783446418530.003SectionsAboutPDF ToolsAdd to FavoritesDownload CitationTrack CitationsCopy LTI LinkPDF key 'share (en)' returned an object instead of string.FacebookTwitterEmailLinkedIn previous chapternext chapter FiguresReferencesRelatedDetails 2008Pages: 55-73Print ISBN: 978-3-446-41315-3eISBN: 978-3-446-41853-0 Copyright & Permissions© 2008 Carl Hanser Verlag GmbH & Co. KGPDF downloadLoading ...
Emergent Disorder and Mechanical Memory in Periodic Metamaterials (2022)

Ordered mechanical systems typically have one or only a few stable rest configurations, and hence are not considered useful for encoding memory. Multistable and history-dependent responses usually emerge from quenched disorder, for example in amorphous solids or crumpled sheets. In contrast, due to geometric frustration, periodic magnetic systems can create their own disorder and espouse an extensive manifold of quasi-degenerate configurations. Inspired by the topological structure of frustrated artificial spin ices, we introduce an approach to design ordered, periodic mechanical metamaterials that exhibit an extensive set of spatially disordered states. While our design exploits the correspondence between frustration in magnetism and incompatibility in meta-mechanics, our mechanical systems encompass continuous degrees of freedom, and are hence richer than their magnetic counterparts. We show how such systems exhibit non-Abelian and history-dependent responses, as their state can depend on the order in which external manipulations were applied. We demonstrate how this richness of the dynamics enables to recognize, from a static measurement of the final state, the sequence of operations that an extended system underwent. Thus, multistability and potential to perform computation emerge from geometric frustration in ordered mechanical lattices that create their own disorder.
Multi-layer and Multi-domain Network Orchestration and Provision of Virtual Views to Users. (2016)

Recently, there are expectations for transport networks among wide area to provide paths dynamically applying Software-Defined Networking (SDN) for faster provisioning paths with low latency and high availability. However, transport networks are actually heterogeneous networks consisting of multiple layers and domains unlike data center networks where SDN have been applied. In addition, virtualization of network resources is also expected by applying SDN. We propose a network orchestrator based on ODENOS for orchestrating multi-layer and multi-domain networks and for providing virtual views to users. Proposed network orchestrator has four features. Firstly, a common data model is introduced for solving a problem of differences among layers and domains. Secondly, relations between layers/domains are managed for solving a problem about lack of managing relations between layers/domains. Thirdly, data model and interfaces are converted between the network orchestrator and layer/domain for solving a problem of differences of methods of path setup among layers/domains. Lastly, a virtual view is created for each user with separated topology based on an ID of each user for solving a problem about lack of a method to create separated views for each user. Results of performance evaluations show that the proposed network orchestrator can dynamically provide virtual views to users on demand.
T cell-dependent hapten-specific and polyclonal B cell responses require release of interleukin 5. (1988)

CD4+ T cells in the mouse have recently been subdivided into two major subpopulations which differ in their functional activities and in the lymphokines they produce. Although cloned T cells lines representative of both sets will activate B cells in polyclonal responses, only the subset producing interleukin 4 (IL-4) will activate antigen-specific B cells in linked recognition assays. This suggested that IL-4 was essential for such responses. In the present experiments, the requirements were compared for B cell activation in specific as opposed to polyclonal antibody responses by T cell clones of the helper (IL-4 producing) subset. It was found that specific responses involve primarily small B cells, whereas polyclonal responses activate exclusively the large B cells. Second, polyclonal B cell responses can proceed in the absence of T:B contact, whereas specific responses require physical interaction of the two cells. Third, it was found that interleukin 5 (IL-5, formerly known as T cell replacing factor/B cell growth factor II) is essential for these polyclonal responses by inhibition of such responses with monoclonal anti-IL-5 antibody. Anti-IL-5 also inhibits specific antibody responses involving direct T:B interaction. Thus, IL-5 is clearly a critical mediator of differentiation to immunoglobulin secretion of activated B cells, whether such B cells are obtained as large B cells from freshly isolated spleen cells or are initially activated in an IL-4-dependent fashion by cognate interaction by a helper T cell clone.
ChemInform Abstract: DIE THERMISCHE UMLAGERUNG UND RINGINVERSION DES 1‐METHOXYHOMOTROPYLIUM‐KATIONS (1973)

Abstract Die Extraktion von Methoxycyclooctatetraen (I) aus CD 2 Cl 2 in Fluorsulfonsäure bei ‐78°C liefert ausschließlich (II).
Fulminant Acute Liver and Heart Failure from Hepatosplenic T-Cell Lymphoma (2013)

Hepatosplenic T-cell lymphoma is a rare extranodal lymphoma, involving primarily the spleen and liver of young male adults. Diffuse lymphoma cell infiltration can cause moderate liver impairment, yet acute liver or even multiorgan failure is an infrequent initial manifestation.We report on a case of a 71-year-old woman, who presented with acute liver failure, anemia, lymphopenia and thrombocytopenia. Previous medication with clindamycin for diarrhea was suggestive for drug-induced liver failure. However, due to elevated lactate dehydrogenase (LDH), fever, hypoproteinemia, elevated sCD25 and lack of other hepatic disorders including any infectious disease, lymphoma was suspected. On CT scan lymphadenopathy was excluded. Thus, due to hepatosplenomegaly, hepatosplenic T-cell lymphoma was assumed. While histopathological analysis was still pending, the patient´s condition rapidly deteriorated. Cardiorespiratory failure necessitated mechanical ventilation on day 5 after admission. The patient died some hours later in catecholamine-refractory cardiac shock. On autopsy, widespread systemic disease with infiltration of heart, liver, spleen, and bone marrow became apparent with intravascular manifestation in the kidneys and lungs. Infiltration of the myocardium had resulted in acute cardiac failure. As a characteristic of hepatosplenic T-cell lymphoma, molecular pathology and cytogenetic analysis revealed a monoclonal γ T-cell receptor and an immunofluorescence staining compatible with isochromosome 7q in 20% and trisomy of chromosome 8 in 35% of tumor cells.
Dual Foucault Pendulum gyroscope (2015)

We report a new type of MEMS degenerate mode lumped mass gyroscope architecture. The Dual Foucault Pendulum (DFP) gyroscope consists of two dynamically equivalent, mechanically coupled proof masses, oscillating in anti-phase motion. This dual axis tuning fork behavior creates a dynamically balanced resonator with x-y symmetry in frequency and damping. Phase synchronization is established by mechanical coupling of the two proof masses, whereas quadrature suppression is achieved by four differential shuttle pairs placed in-between. Dual axis tuning fork behavior provides vibration immunity and anchor loss mitigation, resulting in a Q-factor over 300,000 on both modes at a center frequency of 3.2 kHz. Due to high-Q degenerate mode operation, open-loop performance of 0.003 °/√h ARW and 0.27 °/h in-run bias stability were experimentally demonstrated. We believe Dual Foucault Pendulum is the minimal realization of a dynamically balanced lumped mass degenerate mode gyroscope.
Weighted Ensemble Object Detection with Optimized Coefficients for Remote Sensing Images (2020)

The detection of objects in very high-resolution (VHR) remote sensing images has become increasingly popular with the enhancement of remote sensing technologies. High-resolution images from aircrafts or satellites contain highly detailed and mixed backgrounds that decrease the success of object detection in remote sensing images. In this study, a model that performs weighted ensemble object detection using optimized coefficients is proposed. This model uses the outputs of three different object detection models trained on the same dataset. The model’s structure takes two or more object detection methods as its input and provides an output with an optimized coefficient-weighted ensemble. The Northwestern Polytechnical University Very High Resolution 10 (NWPU-VHR10) and Remote Sensing Object Detection (RSOD) datasets were used to measure the object detection success of the proposed model. Our experiments reveal that the proposed model improved the Mean Average Precision (mAP) performance by 0.78%–16.5% compared to stand-alone models and presents better mean average precision than other state-of-the-art methods (3.55% higher on the NWPU-VHR-10 dataset and 1.49% higher when using the RSOD dataset).