986 CLINICOPATHOLOGICAL IMPACT OF NOVEL BIOMARKERS FOR DIAGNOSIS AND PROGNOSIS OF RENAL CELL CARCINOMA (2012)

You have accessJournal of UrologyKidney Cancer: Evaluation and Staging III1 Apr 2012986 CLINICOPATHOLOGICAL IMPACT OF NOVEL BIOMARKERS FOR DIAGNOSIS AND PROGNOSIS OF RENAL CELL CARCINOMA Christian Thomas, Ladan Fazli, Frederik Roos, Estelle Li, Janet Liew, Joachim Thueroff, Martin Gleave, Walburgis Brenner, and Alan So Christian ThomasChristian Thomas Mainz, Germany More articles by this author , Ladan FazliLadan Fazli Vancouver, Canada More articles by this author , Frederik RoosFrederik Roos Mainz, Germany More articles by this author , Estelle LiEstelle Li Vancouver, Canada More articles by this author , Janet LiewJanet Liew Vancouver, Canada More articles by this author , Joachim ThueroffJoachim Thueroff Mainz, Germany More articles by this author , Martin GleaveMartin Gleave Vancouver, Canada More articles by this author , Walburgis BrennerWalburgis Brenner Mainz, Germany More articles by this author , and Alan SoAlan So Vancouver, Canada More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.1086AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Despite surgery for curative intent, up to 30% of localized renal cell carcinomas (RCC) will progress. The use of tumor-associated biomarkers may be a promising approach to enhance RCC prognostication and guide the use and development of new therapies. The aim of this study was to evaluate the expression levels of 10 different molecular markers for diagnosis and prognosis in patients undergoing radical nephrectomy (RN) for RCC. METHODS The study included 194 patients that underwent RN for clinically diagnosed renal tumor. Immunohistochemical expression levels of 10 proteins, including heat-shock protein 27 (HSP 27), clusterin, Ki-67, ADAM10, carbonic anhydrase XI (CA XI), chemokine CXCL 16, insulin-like growth factor 1-receptor (IGF1R), phosphatase and tensin homolog (PTEN), transcription factors Stat3 and Stat5, were evaluated in a tissue microarray that was developed from tumor samples. Expression profiles were correlated to histology, pathological stage and grade of RN specimen. Stepwise multivariate cox-regression analysis was used to identify independent prognostic factors for cancer-specific survival in clear cell RCC. RESULTS Final pathology of RN specimen revealed 182 RCCs (79.1% clear cell, 13.2% papillary, 7.7% chromophobe) and 12 oncocytomas. Expression levels of clusterin (6.8-fold) and Ki-67 (3.6-fold) were significantly higher in chromophobe RCCs compared to oncocytomas (6.8-fold and 3.6-fold, respectively), whereas Stat5 and IGF1R were significantly lower expressed (2.2-fold and 2.4-fold, respectively) in chromophobe RCCs compared to oncocytomas. Stat5 and IGF1R expression levels were significantly lower in pT-stage ≥ 3 compared to pT-stage ≤ 2. Ki-67, ADAM10 and Stat3 expression levels were signficanlty higher in grade 3-4 compared to grade 1-2 tumors. In stepwise multivariate cox regression analysis, pT-stage ≥ 3 (HR 5.1), grade ≥ 3 (HR3.3), low expression levels of CA XI (HR 2.8) and high expression levels of Ki-67 (HR 3.5) were negative prognosticators for cancer-specific survival in clear cell RCC. CONCLUSIONS Expression levels of several molecular markers tend to correlate with pT-stage and grade. As well, Ki-67 and CA XI appear to be independent prognostic markers and adds to enhance clear cell RCC outcome prediction. Finally, clusterin, Ki-67, Stat5 and IGF1R seem to be useful biomarkers for discrimination between oncocytoma and chromophobe RCC. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e401 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Christian Thomas Mainz, Germany More articles by this author Ladan Fazli Vancouver, Canada More articles by this author Frederik Roos Mainz, Germany More articles by this author Estelle Li Vancouver, Canada More articles by this author Janet Liew Vancouver, Canada More articles by this author Joachim Thueroff Mainz, Germany More articles by this author Martin Gleave Vancouver, Canada More articles by this author Walburgis Brenner Mainz, Germany More articles by this author Alan So Vancouver, Canada More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...