gebhart/patrepeval huggingface dataset:
Data from Cytochrome <i>P</i>450 Epoxygenase Promotes Human Cancer Metastasis (2023)

&lt;div&gt;Abstract&lt;p&gt;Cytochrome &lt;i&gt;P&lt;/i&gt;450 (CYP) epoxygenases convert arachidonic acid to four regioisomeric epoxyeicosatrienoic acids (EET), which exert diverse biological activities in a variety of systems. We previously reported that the CYP2J2 epoxygenase is overexpressed in human cancer tissues and cancer cell lines and that EETs enhance tumor growth, increase carcinoma cell proliferation, and prevent apoptosis of cancer cells. Herein, we report that CYP epoxygenase overexpression or EET treatment promotes tumor metastasis independent of effects on tumor growth. In four different human cancer cell lines &lt;i&gt;in vitro&lt;/i&gt;, overexpression of CYP2J2 or CYP102 F87V with an associated increase in EET production or addition of synthetic EETs significantly induced Transwell migration (4.5- to 5.5-fold), invasion of cells (3- to 3.5-fold), cell adhesion to fibronectin, and colony formation in soft agar. In contrast, the epoxygenase inhibitor 17-ODYA or infection with the antisense recombinant adeno-associated viral vector (rAAV)-CYP2J2 vector inhibited cell migration, invasion, and adhesion with an associated reduction in EET production. CYP overexpression also enhanced metastatic potential &lt;i&gt;in vivo&lt;/i&gt; in that rAAV-CYP2J2–infected MDA-MB-231 human breast carcinoma cells showed 60% more lung metastases in athymic BALB/c mice and enhanced angiogenesis in and around primary tumors compared with control cells. Lung metastasis was abolished by infection with the antisense rAAV-CYP2J2 vector. CYP epoxygenase overexpression or EET treatment up-regulated the prometastatic matrix metalloproteinases and CD44 and down-regulated the antimetastatic genes &lt;i&gt;CD82&lt;/i&gt; and &lt;i&gt;nm-23&lt;/i&gt;. Together, these data suggest that CYP epoxygenase inhibition may represent a novel approach to prevent metastasis of human cancers. [Cancer Res 2007;67(14):6665–74]&lt;/p&gt;&lt;/div&gt;
Phosphorylation of the (Na,K)-ATPase by a plasma membrane-bound protein kinase in friend erythroleukemia cells. (1983)

A decrease in the activity of the (Na,K)-ATPase is an early and essential step in commitment of Friend virus-infected murine erythroleukemia cells to terminal erythroid differentiation. Plasma membranes from these cells were purified and shown to contain ouabain-inhibitable (Na,K)-ATPase present as approximately 0.4% of the total membrane protein. Protein kinase activity also co-purified with the plasma membrane and preferentially phosphorylated a Nonidet P-40 detergent-extractable 100,000-Da peptide. The 100,000-Da phosphopeptide migrated with the alpha subunit of dog kidney (Na,K)-ATPase when electrophoresis was carried out in the presence of sodium dodecyl sulfate in either 5 or 10% polyacrylamide gels. In two-dimensional gel electrophoresis, it separated into a series of spots between pH 5.1 and 5.4, while dog kidney alpha subunit appeared as a doublet at pH 5.3-5.4. When Nonidet P-40-solubilized plasma membranes were passed through a ouabain affinity column in the presence of Mg2+, Na+, and ATP, the 100,000-Da phosphopeptide was retained and could be eluted by ouabain. This peptide was also phosphorylated in living murine erythroleukemia cells, and proteolysis patterns of the peptide labeled in vitro, the peptide labeled in vivo, and the purified dog kidney alpha subunit using V8 protease were nearly identical. Phosphothreonine was detected in both the peptides labeled in vivo and in vitro.
Deposition, Retention, and Translocation of Ultrafine Particles from the Central Airways and Lung Periphery (2008)

Within the limits of detection of our experimental system, most inhaled ultrafine carbon particles are retained in the lung periphery and in the conducting airways without substantial systemic translocation or accumulation in the liver at 48 hours. Repeated exposure may result in significant pulmonary accumulation of ultrafine particles.
32P-labellingof haemoglobin messenger and other reticulocyte ribonucleic acids with polynucleotide phosphokinase in viro (1969)

Research Article| September 01 1969 32P-labellingof haemoglobin messenger and other reticulocyte ribonucleic acids with polynucleotide phosphokinase in viro F. Labrie; F. Labrie 1Medical Research Council Laboratory of Molecular Biology, Cambridge Search for other works by this author on: This Site PubMed Google Scholar F. Sanger F. Sanger 1Medical Research Council Laboratory of Molecular Biology, Cambridge Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1969) 114 (2): 29P. https://doi.org/10.1042/bj1140029Pa Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Cite Icon Cite Get Permissions Citation F. Labrie, F. Sanger; 32P-labellingof haemoglobin messenger and other reticulocyte ribonucleic acids with polynucleotide phosphokinase in viro. Biochem J 1 September 1969; 114 (2): 29P. doi: https://doi.org/10.1042/bj1140029Pa Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu nav search search input Search input auto suggest search filter All ContentAll JournalsBiochemical Journal Search Advanced Search This content is only available as a PDF. © 1969 The Biochemical Society1969 Article PDF first page preview Close Modal You do not currently have access to this content.
Multilevel diffractive lens in the MWIR with extended depth-of-focus and wide field-of-view (2023)

Optics in the mid-wave-infra-red (MWIR) band are generally heavy, thick and expensive. Here, we demonstrate multi-level diffractive lenses; one designed using inverse design approaches and another using the conventional propagation phase (the Fresnel zone plate or FZP) with diameter = 25um and focal length = 25um operating at $\lambda=4\thinspace\mu$m. We fabricated the lenses by optical lithography and characterized their performance. We show that the inverse-designed MDL achieves larger depth-of-focus and better off-axis performance when compared to the the FZP at the expense of larger spot size and reduced focusing efficiencies. Both lenses are flat with thickness &lt;=0.5mm and weigh &lt;= 3.63g, which are far smaller than their conventional refractive counterparts.
Three-Dimensional Light Manipulation by Gold Nanobumps (2013)

Gold nanobumps on the surface of a gold thin film would scatter surface plasmon wave to forward and backward scattering light. Both dot array and chains of gold nanobumps are built to demonstrate the light-manipulating properties. While fan-shaped forward scattering light from individual nanobump is observed, three-dimensional (3-D) light focusing and diverging properties is found from quarter circle structure composed of nanobumps. Nanobumps with their heights of 16nm can deflect the surface plasmon waves to produce 3-D focusing at 3.6 micron above the surface of gold film. Experiment results clearly demonstrate that 3-D forward and backward focusing from gold nanobumps are with different amplitudes and directions of the vertical propagation vectors.
Bead-Based Fluid Array Detection of Pentaerythritol Tetranitrate: Comparison of Monoclonal vs. Llama Polyclonal Antibodies (2010)

In today's security conscious environment rapid detection of explosives, such as pentaerythritol tetranitrate (PETN), the high energy material common in plastic explosives, is of critical importance. We evaluated two monoclonal antibodies and a rabbit polyclonal antibody, developed for the detection of PETN. Although these antibodies showed binding to bioconjugates of PETN as well as the hapten, PETN-succinate, we did not observe binding by free PETN. For demonstration of a competitive bead based fluid array immunoassay for PETN detection, we developed llama polyclonal antibody for the detection of free PETN. Our ultimate goal is developing anti-PETN single domain antibodies.
Effective Heat Dissipation from Color-Converting Plates in High-Power White Light Emitting Diodes by Transparent Graphene Wrapping (2015)

We have developed a hybrid phosphor-in-glass plate (PGP) for application in a remote phosphor configuration of high-power white light emitting diodes (WLEDs), in which single-layer graphene was used to modulate the thermal characteristics of the PGP. The degradation of luminescence in the PGP following an increase in temperature could be prevented by applying single-layer graphene. First, it was observed that the emission intensity of the PGP was enhanced by about 20% with graphene wrapping. Notably, the surface temperature of the graphene-wrapped PGP (G-PGP) was found to be higher than that of the bare PGP, implying that the graphene layer effectively acted as a heat dissipation medium on the PGP surface to reduce the thermal quenching of the constituent phosphors. Moreover, these experimental observations were clearly verified through a two-dimensional cellular automata simulation technique and the underlying mechanisms were analyzed. As a result, the proposed G-PGP was found to be efficient in maintaining the luminescence properties of the WLED, and is a promising development in high power WLED applications. This research could be further extended to generate a new class of optical or optoelectronic materials with possible uses in a variety of applications.
A digest of capillary electrophoretic methods applied to lipid analyzes (2018)

Lipids are naturally occurring organic compounds that can be classified into a number of types based on their solubility in nonpolar organic solvents, and are generally insoluble in water. The great structural variety of these various types of lipids has led them to be components of many different biological substances such as oils, waxes, cellular membranes, tissues and biological fluids. The use of capillary electrophoresis (CE) for the study of lipids during the past 30 years has been relatively rare when compared to its use for other classes of biomolecules, primarily due to their insolubility in water. However, a number of interesting studies have been conducted, and as part of this review, we will present the different approaches that were used, which mainly consist of micellar kinetic chromatography and non-aqueous CE. The main advantages of the use of these techniques compared to GC is the very simple sample preparation that is required and, compared to LC, the very robust and quick separations that can be obtained. In this review, we present the various methods that have been reported in the literature that have been used for the study of fatty acids, phospholipids, glycerides, eicosanoids and sterols, with the inclusion of various tables presenting descriptions of the CE methods used as well as the methods of detection, including UV absorbance, fluorescence, mass spectrometry, and conductivity. This review aims to demonstrate that CE can be easily used for the analysis of lipids.
Advanced small cell carcinoma of the bladder: clinical characteristics, treatment patterns and outcomes in 960 patients and comparison with urothelial carcinoma (2015)

To describe the clinical characteristics, treatment patterns and outcomes in advanced small cell bladder cancer (aSCBC) patients and compare to those with urothelial carcinoma (UC). Individuals in the National Cancer Data Base with a diagnosis of either nodal (TxN+M0) or distant metastatic (TxNxM1) disease were identified from 1998 to 2010. We assessed the relationships between stage, treatment modalities and survival in the aSCBC cohort and compared these to UC patients. In the 960 patient aSCBC cohort (62% M1), 50% received palliative therapy alone, 68% in M1 versus 21% in M0 groups (P < 0.0001). Single modality local therapy (15%) and surgical (21%) or radiation-based (14%) multimodal therapy (MMT) were used in the other 50%. Cystectomy-based MMT was utilized in 45% of N+M0 versus 6.4% of NxM1 patients (P < 0.0001). Median overall survival (OS) for aSCBC patients was 8.6 months; 13.0 months in N+M0 versus 5.3 months in NxM1 patients (P < 0.0001). Survival was similar between TxN1M0 and TxN2-3M0 patients (14.8 months vs. 12.1 months, P = 0.15). Urothelial carcinoma patients (n = 27,796, 45% M1) lived longer compared to aSCBC patients in the N+M0 group (17.3 months vs. 13.0 months, P = 0.0007). There were not clinically significant differences in OS between UC and aSCBC patients in the M1 group. Advanced SCBC is a rare disease with a poor survival and palliative therapy is common, especially in M1 patients. In comparison to UC, the outcomes for aSCBC patients are worse in those with lymph node only involvement but similar in those with distant disease.
Data Plane and Control Architectures for 5G Transport Networks (2016)

Next generation 5G mobile system will support the vision of connecting all devices that benefit from a connection, and support a wide range of services. Consequently, 5G transport networks need to provide the required capacity, latency, and flexibility in order to integrate the different technology domains of radio, transport, and cloud. This paper outlines the main challenges, which the 5G transport networks are facing and discusses in more detail data plane, control architectures, and the tradeoff between different network abstraction models.
Suspended Membrane InGaAs Photonic Crystal Waveguides for Ammonia Sensing at λ=6.15μm (2019)

Fully suspended InGaAs waveguide devices with holey photonic crystal waveguides (HPCWs) are designed for mid-infrared sensing at λ=6.15μm in the low index contrast InGaAs-InP platform. We experimentally detect 5ppm ammonia in 1mm long suspended HPCWs. © 2019 The Author(s)
Positron Emission Tomography (2012)

Positron emission tomography (PET) has been utilized in the clinical evaluation of gastrointestinal (GI) malignancies since the late 1990s. Many GI tumors show enhanced uptake of 2-deoxy-2 [18F] fluoro-D-glucose (FDG), allowing for their localization on a PET scan. Two of the early problems with PET included the relative lack of specificity, as inflammatory processes may also concentrate FDG, and lack of anatomical localization. Both issues have been dramatically improved with the advent of combination PET/CT systems. These hybrid scanners have virtually replaced the original PET cameras. This chapter will highlight the use of FDG-PET in the diagnosis, staging, restaging, and monitoring of treatment response in GI tumors of the esophagus, stomach, small intestine, colon, pancreas, liver, and gallbladder. Additionally, brief mention will be made of the role of PET in patients with GI lymphoma, renal cancer, and adrenal malignancy. Preliminary studies have been done on the use of PET in inflammatory/infectious diseases of the GI tract, and research continues on the development of new radiopharmaceuticals, targeting cellular processes other than glucose metabolism.
NET-PGM extension: SRv6 uSID illustration (2020)

This document illustrates the SRv6 micro segment (SRv6 uSID or uSID
for short) instruction.
Omicron BA.2 breakthrough infection enhances cross-neutralization of BA.2.12.1 and BA.4/BA.5 (2022)

BNT162b2-vaccinated individuals after Omicron BA.1 breakthrough infection have strong serum-neutralizing activity against Omicron BA.1, BA.2, and previous SARS-CoV-2 variants of concern (VOCs) yet less against the highly contagious Omicron sublineages BA.4 and BA.5 that have displaced previous variants. Because the latter sublineages are derived from Omicron BA.2, we characterized serum-neutralizing activity of COVID-19 mRNA vaccine triple-immunized individuals who experienced BA.2 breakthrough infection. We demonstrate that sera of these individuals have broadly neutralizing activity against previous VOCs and all tested Omicron sublineages, including BA.2-derived variants BA.2.12.1 and BA.4/BA.5. Furthermore, applying antibody depletion, we showed that neutralization of BA.2 and BA.4/BA.5 sublineages by BA.2 convalescent sera is driven to a considerable extent by antibodies targeting the N-terminal domain (NTD) of the spike glycoprotein. However, neutralization by Omicron BA.1 convalescent sera depends exclusively on antibodies targeting the receptor binding domain (RBD). These findings suggest that exposure to Omicron BA.2, in contrast to BA.1 spike glycoprotein, triggers substantial NTD-specific recall responses in vaccinated individuals and thereby enhances the neutralization of BA.4/BA.5 sublineages. Given the current epidemiology with a predominance of BA.2-derived sublineages such as BA.4/BA.5 and rapidly ongoing evolution, these findings helped to inform development of our Omicron BA.4/BA.5-adapted vaccine.
Rescoring Confusion Networks for Keyword Search (2014)

We introduce a two-stage cascaded scheme to rescore Confusion Networks (CNs) for Keyword Search in the context of Low-Resource Languages. In the first stage we rescore the CN to improve the error rate of the 1-best hypothesis using a large number of lexical, phonetic, false alarms and structural features. Using a rank learning Support Vector Machine classifier, we obtain WER gains between 0.54% and 2.84% on Cantonese, Tagalog, Turkish, Pashto and Vietnamese. In the second stage we generate keyword hits from the rescored CN and use logistic regression to detect true hits and false alarms. We compare these to hits generated from the unrescored CN and obtain gains between 0.45% and 0.9% on the MTWV metric by using the mentioned features and including acoustic and prosodic features on Tagalog, Turkish and Pashto.
Attractive and repulsive optical gradient forces between silicon waveguides (2009)

I. Abstract Besides optical cooling, optomechanics also provides an exciting and promising way to realize various optically tunable integrated components on a chip. Recently significant progress has been made to control gradient optical forces on a chip [1]. Here we present modeling, fabrication and characterization of a device which enables us to demonstrate attractive and repulsive optical gradient forces between two freestanding nanophotonic silicon waveguides on a Silicon-on-Insulator chip.
The MEERKATS Cloud Security Architecture (2012)

MEERKATS is a novel architecture for cloud environments that elevates continuous system evolution and change as first-rate design principles. Our goal is to enable an environment for cloud services that constantly changes along several dimensions, toward creating an unpredictable target for an adversary. This unpredictability will both impede the adversary's ability to achieve an initial system compromise and, if a compromise occurs, to detect, disrupt, and/or otherwise impede his ability to exploit this success. Thus, we envision an environment where cloud services and data are constantly in flux, using adaptive (both proactive and reactive) protection mechanisms and distributed monitoring at various levels of abstraction. A key element of MEERKATS is the focus on both the software and the data in the cloud, not just protecting but leveraging both to improve mission resilience. MEERKATS seeks to effectively exploit "economies of scale" (in resources available) to provide higher flexibility and effectiveness in the deployment and use of protection mechanisms as and where needed, focusing on current and anticipated application and mission needs instead of an inefficient, "blanket" approach to protecting "everything the same way, all the time". We outline our vision for MEERKATS and describe our approach toward prototyping it.
Arquitetura de resolução mista para codificação distribuída de sequências multiview (2009)

Resumo-Este trabalho apresenta uma nova arquitetura de codificac o distribuda para sequncias multiview, baseada no padro H.264/AVC e em quadros de resoluc o mista. A arquitetura permite transferir a complexidade da codificac o para a decodificac o, sendo particularmente adequada para aplicac es de baixo consumo de energia, como em sistemas de seguranc a com m ltiplas cmeras para uma mesma cena. Explora-se a correlac o espacial e temporal na decodificac o para melhorar a qualidade final do vdeo. Os resultados demonstram potencial de ganho de qualidade objetiva em relac o codificac o independente de cada vista, sem custo adicional de taxa.
Room-temperature-deposited dielectrics and superconductors for integrated photonics (2017)

We present an approach to fabrication and packaging of integrated photonic devices that utilizes waveguide and detector layers deposited at near-ambient temperature. All lithography is performed with a 365 nm i-line stepper, facilitating low cost and high scalability. We have shown low-loss SiN waveguides, high-Q ring resonators, critically coupled ring resonators, 50/50 beam splitters, Mach-Zehnder interferometers (MZIs) and a process-agnostic fiber packaging scheme. We have further explored the utility of this process for applications in nonlinear optics and quantum photonics. We demonstrate spectral tailoring and octave-spanning supercontinuum generation as well as the integration of superconducting nanowire single photon detectors with MZIs and channel-dropping filters. The packaging approach is suitable for operation up to 160 Â°C as well as below 1 K. The process is well suited for augmentation of existing foundry capabilities or as a stand-alone process.
Subcritical Pipe Flow Transition Control Using Dielectric Barrier Discharge Plasma Actuators (2009)

This work details the application of dielectric barrier discharge (DBD) actuators to the control of transition in subcritical Reynolds number (250 ≤ Re ≤ 1000) circular pipe flows for the purposes of increasing mixing or momentum and heat transfer. Primary flow measurements were made using a single hot-wire anemometer and these were augmented using smoke filament visualization with high-speed photography. A sensitive balance was used to calibrate the body force generated by the actuator as a function of input power. Several distinct actuator configurations were considered and a downselection indicated that the introduction of swirl produced the largest coherent oscillations and purely turbulent fluctuations. Using the swirl actuator, a detailed parametric study was conducted where operation parameters such as duty cycle, input power, frequency, momentum, etc. were systematically varied. The largest coherent oscillations occurred at a reduced frequency of approximately 0.08 while peak noncoherent disturban...
Cationic Nanoparticle-Mediated Activation of Natural Killer Cells for Effective Cancer Immunotherapy (2020)

Natural killer (NK) cells have been recognized as a next-generation therapy for cancer as they are less likely to trigger adverse events (<i>e.g</i>., cytokine storm or graft-versus-host disease) than T cell-based therapeutics. Although NK cell activation strategies through genetic engineering and cytokine treatment have been actively studied for successful cancer treatment, the approaches are inefficient, expensive, and involve complex processing. Here, we developed a facile and efficient method of activating NK cells using cationic nanoparticles (cNPs). The cytotoxic activity of cNP-treated primary NK and NK-92MI cells against triple-negative breast cancer cells was over 2-fold higher than that of control NK cells <i>in vitro</i>. Molecular biological analyses confirmed that cNPs altered the expression of CCR4 and CXCR4 of NK cells that function as chemokine receptors. <i>In vitro</i> live cell imaging showed that the NK cells treated with cNPs were better than control NK cells at interacting with cancer cells. Consistent with these <i>in vitro</i> results, cNP-treated NK cells effectively inhibited tumor growth in an <i>in vivo</i> tumor animal model of triple-negative breast cancer. Additionally, NK cells treated with cNPs were tracked effectively <i>in vivo</i> by magnetic resonance imaging. Thus, cNP-mediated activation of NK cells has great potential as an NK cell-based cancer immunotherapy. Most of all, activating NK cells using cNPs has a great advantage over conventional methods in that immune cells can be activated by a one-step facile process with exogenously charged nanomaterials, without the need for genetic engineering or cytokine treatment.