gebhart/patrepeval huggingface dataset:
The Bone-Forming Properties of Periosteum-Derived Cells Differ Between Harvest Sites (2020)

The development of alternatives for autologous bone grafts is a major focus of bone tissue engineering. To produce living bone-forming implants, skeletal stem and progenitor cells (SSPCs) are envisioned as key ingredients. SSPCs can be obtained from different tissues including bone marrow, adipose tissue, dental pulp, and periosteum. Human periosteum-derived cells (hPDCs) exhibit progenitor cell characteristics and have well-documented <i>in vivo</i> bone formation potency. Here, we have characterized and compared hPDCs derived from tibia with craniofacial hPDCs, from maxilla and mandible, respectively, each representing a potential source for cell-based tissue engineered implants for craniofacial applications. Maxilla and mandible-derived hPDCs display similar growth curves as tibial hPDCs, with equal trilineage differentiation potential toward chondrogenic, osteogenic, and adipogenic cells. These craniofacial hPDCs are positive for SSPC-markers CD73, CD164, and Podoplanin (PDPN), and negative for CD146, hematopoietic and endothelial lineage markers. Bulk RNA-sequencing identified genes that are differentially expressed between the three sources of hPDC. In particular, differential expression was found for genes of the HOX and DLX family, for <i>SOX9</i> and genes involved in skeletal system development. The <i>in vivo</i> bone formation, 8 weeks after ectopic implantation in nude mice, was observed in constructs seeded with tibial and mandibular hPDCs. Taken together, we provide evidence that hPDCs show different profiles and properties according to their anatomical origin, and that craniofacial hPDCs are potential sources for cell-based bone tissue engineering strategies. The mandible-derived hPDCs display - both <i>in vitro</i> and <i>in vivo -</i> chondrogenic and osteogenic differentiation potential, which supports their future testing for use in craniofacial bone regeneration applications.
Chemical agent detection by surface-enhanced Raman spectroscopy (2004)

In the past decade, the Unites States and its allies have been challenged by a different kind of warfare, exemplified by the terrorist attacks of September 11, 2001. Although suicide bombings are the most often used form of terror, military personnel must consider a wide range of attack scenarios. Among these is the intentional poisoning of water supplies to obstruct military operations in Afghanistan and Iraq. To counter such attacks, the military is developing portable analyzers that can identify and quantify potential chemical agents in water supplies at microgram per liter concentrations within 10 minutes. To aid this effort we have been investigating the value of a surface-enhanced Raman spectroscopy based portable analyzer. In particular we have been developing silver-doped sol-gels to generate SER spectra of chemical agents and their hydrolysis products. Here we present SER spectra of several chemical agents measured in a generic tap water. Repeat measurements were performed to establish statistical error associated with SERS obtained using the sol-gel coated vials.
Palladium-catalysed alkene chain-running isomerization (2017)

We report a method for palladium-catalysed chain-running isomerization of terminal and internal alkenes. Using an air-stable 2,9-dimethylphenanthroline-palladium catalyst in combination with NaBAr<sub>4</sub> promoter, olefins are converted to the most stable double bond isomer at -30 to 20 °C. Silyl enol ethers are readily formed from silylated allylic alcohols. Fluorinated substituents are compatible with the reaction conditions, allowing the synthesis of fluoroenolates. Catalyst loading as low as 0.05% can be employed on a gram scale.
Pharmacological Topics of Bone Metabolism: Antiresorptive Microbial Compounds That Inhibit Osteoclast Differentiation, Function, and Survival (2008)

The mass and function of bones depends on the maintenance of a complicated balance between osteoclast-mediated bone resorption and osteoblast-mediated bone formation. Osteoporosis typically reflects an imbalance in skeletal turnover, such that bone resorption exceeds bone formation. Osteoclasts are target cells for anti-osteoporosis therapies. To discover new types of antiresorptive agents, we screened for natural compounds that regulate osteoclast differentiation, function, and survival. As a result, we identified reveromycin A, destruxins, mevastatin, FK506, cyclosporin A, prodigiosins, concanamycins, and symbioimine among microbial natural compounds. In this review, we discuss the mechanisms of action of these compounds on osteoclasts.
Mutation analysis of the Bruton's tyrosine kinase gene in X-linked agammaglobulinemia: identification of a mutation which affects the same codon as is altered in immunodeficient xid mice (1994)

X-linked agammaglobulinemia (XLA) is an inherited immunodeficiency disease in man, reflecting an arrest in differentiation of pre-B cells to mature B cell stages. The gene defective in XLA has been identified as a cytoplasmic protein tyrosine kinase, named btk (Bruton's tyrosine kinase). Here we report the characterization of mutations in the btk gene of five unrelated XLA families. Amplified products were generated from cDNA, cloned and sequenced. Three single point mutations and two small insertions were identified. One of the point mutations and the two insertions created stop codons that would lead to truncated btk proteins. In one XLA patient we found a single basepair substitution that altered the highly conserved Arg288 within the SH2 domain and would therefore abrogate interactions with substrate phosphotyrosines. In another XLA patient a single basepair substitution was observed that altered the conserved Arg28 residue in the N-terminal unique region of unknown function. This residue is also mutated in the xid mouse, which has a different, less severe, B cell deficiency. We conclude that a similar mutation in the btk gene leads in man to an almost complete arrest at an early stage of B cell differentiation, but in the mouse to only limited B cell abnormalities.
Practical Recommendations on Crawling Online Social Networks (2011)

Our goal in this paper is to develop a practical framework for obtaining a uniform sample of users in an online social network (OSN) by crawling its social graph. Such a sample allows to estimate any user property and some topological properties as well. To this end, first, we consider and compare several candidate crawling techniques. Two approaches that can produce approximately uniform samples are the Metropolis-Hasting random walk (MHRW) and a re-weighted random walk (RWRW). Both have pros and cons, which we demonstrate through a comparison to each other as well as to the "ground truth." In contrast, using Breadth-First-Search (BFS) or an unadjusted Random Walk (RW) leads to substantially biased results. Second, and in addition to offline performance assessment, we introduce online formal convergence diagnostics to assess sample quality during the data collection process. We show how these diagnostics can be used to effectively determine when a random walk sample is of adequate size and quality. Third, as a case study, we apply the above methods to Facebook and we collect the first, to the best of our knowledge, representative sample of Facebook users. We make it publicly available and employ it to characterize several key properties of Facebook.
Indoor radio communications for factories of the future (1989)

The need for reliable, real-time communication for automated factories is discussed. The ability of narrowband digital radio systems to meet that need is examined. The major problems encountered in multipath propagation, resulted from multiple reflections of the transmitted signal from the building structure and surrounding inventory. Radio wave propagation experiments at 1300 MHz, which were conducted by the author in five operational factories, are described. Other studies of multipath propagation are discussed, and techniques for overcoming it are considered. Research on multiple-access networking is summarized.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
High power 938 nanometer fiber laser and amplifier (2023)

An optical fiber amplifier includes a length of silica optical fiber having a core doped with neodymium, a first cladding and a second cladding each with succeeding lower refractive indices, where the first cladding diameter is less than 10 times the diameter of the core. The doping concentration of the neodymium is chosen so that the small signal absorption for 816 nm light traveling within the core is less than 15 dB/m above the other fiber losses. The amplifier is optically pumped with one laser into the fiber core and with another laser into the first cladding.
Left Atrial Appendage Occlusion (2022)

The field of left atrial appendage occlusion is rapidly evolving. However, several issues remain including the limited randomized efficacy data, peri-device leak, device-related thrombus, and the ongoing refinement of procedural techniques. In this article, we provide a contemporary overview of left atrial appendage occlusion focusing on 4 key remaining challenges: efficacy data, peri-device leak, device-related thrombus, and procedural optimization.
Generative and Discriminative Methods using Morphological Information for Sentence Segmentation of Turkish (2009)

Abstract—This paper presents novel methods for generative, discriminative, and hybrid sequence classification for segmentation of Turkish word sequences into sentences. In the literature, this task is generally solved using statistical models that take advantage of lexical information among others. However, Turkish has a productive morphology that generates a very large vocabulary, making the task much harder. In this paper, we introduce a new set of morphological features, extracted from words and their morphological analyses. We also extend the established method of hidden event language modeling (HELM) to factored hidden event language modeling (fHELM) to handle morphological information. In order to capture non-lexical information, we extract a set of prosodic features, which are mainly motivated from our previous work for other languages. We then employ discriminative classification techniques, boosting and conditional random fields (CRFs), combined with fHELM, for the task of Turkish sentence segmentation. Index Terms—Prosodic and lexical information, sentence segmentation, Turkish morphology. I.
Protein Kinase C Activates Human Lipocalin-type Prostaglandin D Synthase Gene Expression through De-repression of Notch-HES Signaling and Enhancement of AP-2β Function in Brain-derived TE671 Cells (2005)

Here we investigated the regulatory mechanism of lipocalin-type prostaglandin D synthase (L-PGDS) gene expression in human TE671 (medulloblastoma of cerebellum) cells. Reporter analysis of the promoter region from -730 to +75 of the human L-PGDS gene demonstrated that deletion or mutation of the N-box at -337 increased the promoter activity 220-300%. The N-box was bound by Hes-1, a mammalian homologue of Drosophila Hairy and enhancer of split, as examined by electrophoretic mobility shift assay and chromatin immunoprecipitation assay. Functional expression of the Notch intracellular domain significantly increased Hes-1 expression and decreased L-PGDS expression level in TE671 cells. Moreover, knock-down of Hes-1 mRNA by RNA interference significantly enhanced the L-PGDS mRNA level, indicating that the L-PGDS gene expression is repressed by the Notch-Hes signaling. When the AP-2 element at -98 of the promoter region was deleted or mutated, the promoter activity was drastically decreased to approximately 10% of normal. The AP-2 element was bound by AP-2beta dominantly expressed in TE671 cells, according to the results of electrophoretic mobility shift assay and chromatin immunoprecipitation assay. L-PGDS expression was induced by 12-O-tetradecanoylphorbol-13-acetate in TE671 cells, and this induction was inhibited by a protein kinase C inhibitor. Stimulation of TE671 cells with 12-O-tetradecanoylphorbol-13-acetate or transfection with protein kinase Calpha expression vector induced phosphorylation of Hes-1, inhibition of DNA binding of Hes-1 to the N-box, and activation of the AP-2beta function to up-regulate L-PGDS gene expression. These results reveal a novel transcriptional regulatory mechanism responsible for the high level expression of the human L-PGDS gene in TE671 cells.
Variable flux machine torque estimation and pulsating torque mitigation during magnetization state manipulation (2013)

This paper focuses on dynamic control, under loaded conditions, of the magnetization state of suitably designed variable flux (VF) permanent magnet (PM) machines. Such VF-PM machines have been shown to achieve low loss operation over a wide range of load and speed. For this type of machine, the PM flux linkage varies during the magnetization manipulation process. Published magnetization techniques have occurred at zero load conditions and thus did not generate torque pulsations. However, under loaded conditions, the existing methods would produce unwanted torque pulsation. This paper proposes a parameter insensitive method to solve this issue. This method generates a decoupling current command which is calculated from accurately estimated stator flux linkage. Accurate flux estimation, i.e. insensitive to inductance saturation and PM flux linkage variation (e.g. temperature or magnetization level) is achieved by using the voltage disturbance estimated by a closed-loop stator current vector observer. In both simulations and experiments, it is shown that even during magnetization processes under loaded conditions, the flux can be estimated correctly and smooth torque output can be achieved.
The Subvastus Approach for Total Knee Arthroplasty Resulted in Better Short-Term Outcomes than Did the Parapatellar Approach (2002)

Question: In patients having total knee arthroplasty, how do the medial patellar and subvastus approaches compare with regard to functional outcomes and postoperative recovery?

Design: Randomized (unclear allocation concealment), blinded (patients and outcome assessors), controlled trial with 3-month follow-up.

Setting: A district hospital in England.

Patients: 89 patients (mean age, 70 years; 53% men) who had a primary total knee arthroplasty for treatment of osteoarthritis. Follow-up was complete.

Intervention: Patients were allocated to total knee …
Femtosecond X-ray generation in the water window using 28-fs laser pulses (2002)

Summary form only given.Recent advances in laser technology have led to fs X-ray production by high harmonic generation using ultrashort pulses with pulse durations in the range of 5-25 fs. We have shown that the cutoff harmonic photon energy hv, is inversely proportional to the square of the log of the laser pulse duration, and is simply described by an analytical formula.
Excited-State Proton Transfer of Fluorescein Anion as an Ionic Liquid Component (2013)

Fluorescent ionic liquids (FILs) incorporating the fluorescein anion have been prepared by anion exchange of the parent quaternary ammonium chloride (Quat(+)Cl(-)) ionic liquid. By controlling the molar ratio of fluorescein to Quat(+)Cl(-), ionic liquids incorporating different prototropic forms of fluorescein were prepared. The 1:1 molar ratio ionic liquid (FIL1) is essentially composed of monoanionic fluorescein, while dianionic fluorecein is predominant in the FIL with a 1:2 molar ratio (FIL2). The fluorescence excitation spectrum of FIL2 is markedly different from its absorption spectrum. Absorption features the fluorescein dianion, while the excitation spectrum is exclusively due to the monoanion. In FIL1, the absorption and excitation spectra are both characteristic of the monoanion. In both FILs, emission of the dianion is observed upon excitation of the monoanion. This unusual behavior is interpreted in the context of a fast deprotonation of the monoanion in the excited state. The presence of residual water in the ionic liquid is important for the proton transfer process. By lowering the pH of FIL1, the transient proton transfer is inhibited, and the emission of the monoanion could be observed. The FILs have completely different spectroscopic properties from solvated fluorescein in Quat(+)Cl(-), where the prototropic equilibrium is shifted toward the neutral forms.
Mutational screening of APP gene in patients with early‐onset Alzheimer disease utilizing mismatched PCR‐RFLP (1996)

To elucidate the frequency of mutations of the beta/A4 amyloid protein precursor (APP) gene in early-onset Alzheimer disease, we designed a mismatched PCR-RFLP that can identify all kinds of missense mutations at codon 717 in addition to the seven kinds of known mutations at exon 17. When we screened mutations at exon 17 utilizing this method and the double missense mutations at exon 16 of the APP gene by PCR-RFLP, no cases revealed mutations of the APP gene among 13 familial and 54 sporadic cases, except one family (OS-1) that had previously been reported and used as a positive control of APP717(Val-->Ile). Our results support the hypothesis that mutations in the APP gene are not major causes in early-onset Alzheimer disease.
N-Heterocyclic carbenes (NHCs) as organocatalysts and structural components in metal-free polymer synthesis (2013)

The chemistry of N-heterocyclic carbenes (NHCs) has witnessed tremendous development in the past two decades: NHCs have not only become versatile ligands for transition metals, but have also emerged as powerful organic catalysts in molecular chemistry and, more recently, in metal-free polymer synthesis. To understand the success of NHCs, this review first presents the electronic properties of NHCs, their main synthetic methods, their handling, and their reactivity. Their ability to activate key functional groups (e.g. aldehydes, esters, heterocycles, silyl ketene acetals, alcohols) is then discussed in the context of molecular chemistry. Focus has been placed on the activation of substrates finding analogies with monomers (e.g. bis-aldehydes, multi-isocyanates, cyclic esters, epoxides, N-carboxyanhydrides, etc.) and/or initiators (e.g. hydroxy- or trimethylsilyl-containing reagents) employed in such "organopolymerisation" reactions utilizing NHCs. A variety of metal-free polymers, including aliphatic polyesters and polyethers, poly(α-peptoid)s, poly(meth)acrylates, polyurethanes, or polysiloxanes can be obtained in this way. The last section covers the use of NHCs as structural components of the polymer chain. Indeed, NHC-based photoinitiators, chain transfer agents or functionalizing agents, as well as bifunctional NHC monomer substrates, can also serve for metal-free polymer synthesis.
Supplementary Table S6 from High-Order Drug Combinations Are Required to Effectively Kill Colorectal Cancer Cells (2023)

&lt;p&gt;Summarized results for high-order combinations in DLD-1, RKO, HT-29, and LS-180 cell lines.&lt;/p&gt;
L'enjeu de l'intégration des fonctions amplifications millimétriques dans le silicium nanométrique (2013)

National audience
CRISPR/Cas9-generated models uncover therapeutic vulnerabilities of del(11q) CLL cells to dual BCR and PARP inhibition (2020)

The deletion of 11q (del(11q)) invariably comprises ATM gene in chronic lymphocytic leukemia (CLL). Concomitant mutations in this gene in the remaining allele have been identified in 1/3 of CLL cases harboring del(11q), being the biallelic loss of ATM associated with adverse prognosis. Although the introduction of targeted BCR inhibition has significantly favored the outcomes of del(11q) patients, responses of patients harboring ATM functional loss through biallelic inactivation are unexplored, and the development of resistances to targeted therapies have been increasingly reported, urging the need to explore novel therapeutic approaches. Here, we generated isogenic CLL cell lines harboring del(11q) and ATM mutations through CRISPR/Cas9-based gene-editing. With these models, we uncovered a novel therapeutic vulnerability of del(11q)/ATM-mutated cells to dual BCR and PARP inhibition. Ex vivo studies in the presence of stromal stimulation on 38 CLL primary samples confirmed a synergistic action of the combination of olaparib and ibrutinib in del(11q)/ATM-mutated CLL patients. In addition, we showed that ibrutinib produced a homologous recombination repair impairment through RAD51 dysregulation, finding a synergistic link of both drugs in the DNA damage repair pathway. Our data provide a preclinical rationale for the use of this combination in CLL patients with this high-risk cytogenetic abnormality.
Treatment of Experimental Pneumocystis carinii Pneumonia with Analogs of Pentamidine (1989)

Seven analogs of pentamidine were tested for their activity against an immunosuppressed rat model of Pneumocystis carinii pneumonia. Structural alterations of the pentamidine molecule included variations of the alkyl chain linking the two p-amidino phenoxy moieties and relocation of the amidine groups from the para to the meta position on the phenoxy rings. All analogs of pentamidine were active against P. carinii pneumonia when compared to a saline-treated control group. One derivative, 1, 4-di(4'-amidinophenoxy)butane, proved to be statistically more active than the parent drug.
Fine Sensorless Force Control using Diode-Clamped Linear Amplifiers (2014)

In this paper, diode-clamped linear amplifiers (DCLAs) are developed for achieving low-noise and fine force control. Class-B and Class-D power amplifiers are not suitable for driving a motor, owing to their power efficiency and high switching noise. In particular, a force control system using Class-D power amplifiers cannot generate appropriate fine force because of the switching operations by PWM or PDM. For that reason, we focus on DCLAs, which are capable of solving the problems both power efficiency and force noise. Furthermore, the force noise generated by the linear motor is less than that generated by the Class-D power amplifiers. Through a theoretical analysis, simulations, and experiments, the validity of the force control system using DCLAs is verified.
Amino Acid/Spermine Conjugates:  Polyamine Amides as Potent Spermidine Uptake Inhibitors (2001)

In this paper we describe the synthesis and characterization of a series of simple spermine/amino acid conjugates, some of which potently inhibit the uptake of spermidine into MDA-MB-231 breast cancer cells. The presence of an amide in the functionalized polyamine appeared to add to the affinity for the polyamine transporter. The extensive biological characterization of an especially potent analogue from this series, the Lys-Spm conjugate (31), showed this molecule will be an extremely useful tool for use in polyamine research. It was shown that the use of 31 in combination with DFMO led to a cytostatic growth inhibition of a variety of cancer cells, even when used in the presence of an extracellular source of transportable spermidine. It was furthermore shown that this combination effectively reduced the cellular levels of putrescine and spermidine while not affecting the levels of spermine. These facts together with the nontoxic nature of 31 make it a novel lead for further anticancer development.
A CMOS Low-Power 10:4 MUX and 4:10 DEMUX Gearbox IC for 100-Gigabit Ethernet Link (2011)

The world's first CMOS "gearbox LSI" based on 65-nm CMOS technology-namely, a 2-W 100-gigabit-Ethernet gearbox LSI combining a 10:4 multiplexer and a 4:10 demultiplexer-was developed. Its power consumption is 75% lower than that of a conventional SiGe-based gearbox LSI. The power consumption of its 12.5-Gb/s interface is 0.98 mW/(Gb/s), while that of its 25- Gb/s interface is 14 mW/(Gb/s).