gebhart/patrepeval huggingface dataset:
Modulation by Peroxynitrite of Akt- and AMP-activated Kinase-dependent Ser1179 Phosphorylation of Endothelial Nitric Oxide Synthase (2002)

Peroxynitrite (ONOO(-)), a nitric oxide-derived oxidant, uncouples endothelial nitric oxide synthase (eNOS) and increases enzymatic production of superoxide anions (O(2)()) (Zou, M. H., Shi, C., and Cohen, R. A. (2002) J. Clin. Invest. 109, 817-826). Here we studied how ONOO(-) influences eNOS activity. In cultured bovine aortic endothelial cells (BAEC), ONOO(-) increased basal and agonist-stimulated Ser(1179) phosphorylation of eNOS, whereas it decreased nitric oxide production and bioactivity. However, ONOO(-) strongly inhibited the phosphorylation and activity of Akt, which is known to phosphorylate eNOS-Ser(1179). Moreover, expression of an Akt dominant-negative mutant did not prevent ONOO(-)-enhanced eNOS-Ser(1179) phosphorylation. In contrast to Akt, ONOO(-) significantly activated 5'-AMP-activated kinase (AMPK), as evidenced by its increased Thr(172) phosphorylation as well as increased Ser(92) phosphorylation of acetyl-coenzyme A carboxylase, a downstream target of AMPK. Associated with the increased release of O(2)(), ONOO(-) significantly increased the co-immunoprecipitation of eNOS with AMPK. Further, overexpression of the AMPK-constitutive active adenovirus significantly enhanced ONOO(-) up-regulated eNOS-Ser(P)(1179). In contrast, overexpression of a dominant-negative AMPK mutant attenuated the ONOO(-)-enhanced eNOS-Ser(1179) phosphorylation as well as O(2)() release. We conclude that ONOO(-) inhibits Akt and increases AMPK-dependent Ser(1179) phosphorylation of eNOS resulting in enhanced O(2)() release.
Endometrial ablation for heavy menstrual bleeding (2005)

Both REA and NREA provide at least short- to intermediate-term options to hysterectomy for patients with heavy menstrual bleeding and normal or near-normal endometrial cavities. Consequently, the ideal candidates are likely those who are within 5 years of menopause.
Increased Sensitivity of Love Wave Delay-Lines for Heavy Metal Detection through the Deposition of a Mesoporous TiO2 Coating (2011)

International audience
Zirconocene-coupling routes to conjugated polymers: soluble poly(arylenedienylene)s (1998)

B. L. Lucht and T. Don Tilley, Chem. Commun., 1998, 1645 DOI: 10.1039/A802538J
ChemInform Abstract: Preparation and Assignment of Configuration of cis‐ and trans‐ 2,3,4,4a,5,6‐Hexahydro‐2‐naphthalenol. (1987)

Abstract The naphthalenols (II) and (IV) mentioned in the title are prepared by selective alanate reduction of the ketone (I) or by hydrolysis of the p‐nitrobenzoate (III).
Model-Based Estimation and Control System Development in a Urea-SCR Aftertreatment System (2008)

&lt;div class="htmlview paragraph"&gt;In this paper, a model-based linear estimator and a non-linear control law for an Fe-zeolite urea-selective catalytic reduction (SCR) catalyst for heavy duty diesel engine applications is presented. The novel aspect of this work is that the relevant species, NO, NO&lt;sub&gt;2&lt;/sub&gt; and NH&lt;sub&gt;3&lt;/sub&gt; are estimated and controlled independently. The ability to target NH&lt;sub&gt;3&lt;/sub&gt; slip is important not only to minimize urea consumption, but also to reduce this unregulated emission. Being able to discriminate between NO and NO&lt;sub&gt;2&lt;/sub&gt; is important for two reasons. First, recent Fe-zeolite catalyst studies suggest that NO&lt;sub&gt;x&lt;/sub&gt; reduction is highly favored by the NO 2 based reactions. Second, NO&lt;sub&gt;2&lt;/sub&gt; is more toxic than NO to both the environment and human health. The estimator and control law are based on a 4-state model of the urea-SCR plant. A linearized version of the model is used for state estimation while the full nonlinear model is used for control design. An experimentally validated, higher order simulation is used to evaluate the performance of the closed loop system. For the cases considered, the control strategy uses less urea, produces less NH&lt;sub&gt;3&lt;/sub&gt; slip, and less tailpipe NO&lt;sub&gt;x&lt;/sub&gt; than a similar strategy where NO and NO&lt;sub&gt;2&lt;/sub&gt; are assumed as all NO during estimation and control law implementation.&lt;/div&gt;
Correlations of Body Mass Index, Japan-Defined and IDF-Defined Waist Circumference, Visceral and Subcutaneous Fat Area with Metabolic Parameters in Japanese Patients with Type 2 Diabetes (2015)

Obesity is included in the definition of metabolic syndrome. However, there are many controversies surrounding the evaluation of obesity such as body mass index (BMI) and waist circumference (WC) in the definition of metabolic syndrome among various populations. To understand precisely how various anthropometric indices of obesity influence metabolic parameters, we studied the correlations of BMI, WC (Japanese criteria), WC (IDF criteria), visceral fat area (VFA), subcutaneous fat area (SFA) and the VFA/SFA ratio with metabolic parameters in patients with type 2 diabetes. The influences of BMI and WC on diastolic blood pressure and HDL-cholesterol were larger than VFA, whereas the influences of visceral fat area on triglyceride, very low-density lipoprotein-cholesterol, C-peptide and high-sensitivity C-reactive protein were larger than BMI and WC. WC (IDF) was the strongest determinant of adiponectin among anthropometric indices. Present study showed significant different associations of BMI, Japan- and IDF-defined WC, VFA, SFA and the VFA/SFA ratio with blood pressure, glucose and lipid metabolism, and adipocytokines in Japanese patients with type 2 diabetes.
Biophysical Characterization of the Interactions of HTI-286 with Tubulin Heterodimer and Microtubules (2003)

HTI-286 is a synthetic analogue of the natural product hemiasterlin and is a potent antimitotic agent. HTI-286 inhibits the proliferation of tumor cells during mitosis. The observed antimitotic activity is due to the binding of HTI-286 to tubulin. This report details the effects of HTI-286 on soluble tubulin and preassembled microtubules. HTI-286 binds tubulin monomer and oligomerizes it to an 18.5 S species corresponding to a discrete ring structure consisting of about 13 tubulin units as determined by sedimentation equilibrium analyses. The rate of formation of the oligomers is dependent on the concentration of HTI-286 and the time of incubation. Tubulin oligomers, specifically the 18.5 S species, form slowly. The interactions of HTI-286 with tubulin were studied by isothermal titration calorimetry. HTI-286 binds tubulin rapidly, and the initial association of HTI-286 with tubulin is enthalpically driven with a DeltaH value of -14 kcal/mol at 25 degrees C and a dissociation constant of ca. 100 nM. However, the accompanying tubulin oligomerization event does not produce measurable heats at 25 degrees C. The dissociation constant estimated from the changes in the intrinsic fluorescence of tubulin was found to be consistent with the calorimetric results. Both HTI-286 and hemiasterlin bind tubulin with nearly equal potency. However, the stability of the tubulin oligomers is not identical under size-exclusion column chromatographic conditions. The tubulin oligomers formed in the presence of HTI-286 dissociate on the column, while the corresponding oligomers formed in the presence of hemiasterlin are stable. Tubulin undergoes a change in the secondary structure in the presence of HTI-286, which is evidenced by changes in the circular dichroic absorption spectrum of tubulin. In contrast to the microtubule-stabilizing effects of paclitaxel, both HTI-286 and hemiasterlin depolymerize preassembled microtubules at micromolar concentrations.
Performance and Condemnation Rate Analysis of Commercial Turkey Flocks Treated with a Lactobacillus spp.-Based Probiotic (2007)

The use of defined probiotic cultures in the poultry industry has recently become more common. However, few conclusive studies regarding their efficacy under commercial conditions have been reported in the scientific literature. We conducted a study that included 118 commercial turkey hen lots, ranging from 1,542 to 30,390 hens per lot, of either Nicholas or Hybrid genetic lines, to look at the effect of a selected commercial Lactobacillus-based probiotic (FM-B11) on turkey BW, performance, and health. Sixty lots received the probiotic, whereas 58 lots were controls without probiotic. The probiotic was administered for 3 consecutive days at placement (day of age) and at move-out (around 6 wk of age, movement from brooder to grower houses). The parameters collected, calculated, and analyzed (significance level P < 0.05) were market BW, average daily weight gain, feed conversion ratio, and cost of production. There was no interaction effect between the genetic line and probiotic effect. Therefore, data from the 2 genetic lines were combined for the statistical analysis of the probiotic effect. The probiotic significantly improved market BW and average daily gain by 190 and 1.63 g, respectively. The feed conversion ratio was not statistically different between treatments (2.176 vs. 2.192 for the probiotic and control, respectively). However, the cost of production was lower in the probiotic-treated (58.37 cents/kg of live turkey) than in the control (59.90 cents/kg of live turkey) lots. Condemnation rates were not significantly different between lots. When each premise was compared by level of performance as good, fair, or poor (grouping based on historical analysis of 5 previous flocks), the probiotic appeared to increase the performance of the poor and fair farms. Use of the selected commercial probiotic resulted in increased market BW and reduced cost of production.
Spatial low pass filters for pin actuated tactile displays (2003)

A common tactile display design uses an array of mechanical pins covered by a rubber layer which acts as a spatial low pass filter. To characterize the perceptual relationship between this rubber layer and shape rendering, we conducted psychophysical experiments to examine the perception of a vertical line stimulus felt using rubber covers of varying thickness and stiffness. We found no significant change in perception for rubber thicknesses ranging from 1.5-3.0 mm and for stiffnesses ranging from 45-200 kN/m/sup 2/.
Miniature Two-Axis Actuator for High-Data-Transfer-Rate Optical Data Storage System (2002)

A miniature actuator for optical pickups is desirable not only for downsizing optical data storage systems, but also for increasing the data transfer rate. Since the size of an actuator is essentially determined by the size of the objective lens, recent developments in techniques for fabricating a small high-numerical-aperture (NA) objective lens enable us to develop a miniature actuator. In this paper, we report the successful development of a two-axis actuator using a φ1.5 mm effective aperture objective lens (half the size of the lens we used in our last report on the optical data storage system using a high-numerical-aperture). We evaluated the dynamic characteristics of the actuator by the read/write test using a 23.3 Gbyte phase-change disk. We also confirmed that the actuator satisfies the specifications required for a high-data-transfer-rate system up to 140 Mbps, due to its broad servo bandwidth.
2006 Pathogen and Toxin Concentration Systems for Water Monitoring (2012)

Abstract : Responding to the Department of Defense requests for improvements in the concentration of multiple waterborne pathogens, a proposal was submitted by both parties to design and construct a compact, portable automated device enabling the simultaneous concentration of protozoa, bacteria, bacterial spores, algae and viruses from large volumes of various matrices. The first prototype, named CFC100A, is a portable, selfcontained device and the process of sample concentration and elution is completely automated. It can easily be programmed to concentrate from 1 1000L.
Waste plastics in asphalt concrete: A review (2021)

Abstract Among possible applications for post‐consumer waste plastics, one criterion is that the application should be able to use very large amounts of material because the amount of plastic waste is so large. Another key issue is economics; very little cost should be involved in preparing waste plastics for a specific application. One of the few applications for post‐consumer waste plastics that could possibly meet both requirements is filler in asphalt concrete. Specifically, if waste plastics in asphalt concrete were ∼ 10 wt%, then this application alone could use all plastic waste currently being produced in the world. This review examines the progress that has been made to incorporate waste plastics into asphalt concrete and identifies key issues preventing this technology from being applied commercially.
Human Skin Microbiota: High Diversity of DNA Viruses Identified on the Human Skin by High Throughput Sequencing (2012)

The human skin is a complex ecosystem that hosts a heterogeneous flora. Until recently, the diversity of the cutaneous microbiota was mainly investigated for bacteria through culture based assays subsequently confirmed by molecular techniques. There are now many evidences that viruses represent a significant part of the cutaneous flora as demonstrated by the asymptomatic carriage of beta and gamma-human papillomaviruses on the healthy skin. Furthermore, it has been recently suggested that some representatives of the Polyomavirus genus might share a similar feature. In the present study, the cutaneous virome of the surface of the normal-appearing skin from five healthy individuals and one patient with Merkel cell carcinoma was investigated through a high throughput metagenomic sequencing approach in an attempt to provide a thorough description of the cutaneous flora, with a particular focus on its viral component. The results emphasize the high diversity of the viral cutaneous flora with multiple polyomaviruses, papillomaviruses and circoviruses being detected on normal-appearing skin. Moreover, this approach resulted in the identification of new Papillomavirus and Circovirus genomes and confirmed a very low level of genetic diversity within human polyomavirus species. Although viruses are generally considered as pathogen agents, our findings support the existence of a complex viral flora present at the surface of healthy-appearing human skin in various individuals. The dynamics and anatomical variations of this skin virome and its variations according to pathological conditions remain to be further studied. The potential involvement of these viruses, alone or in combination, in skin proliferative disorders and oncogenesis is another crucial issue to be elucidated.
Adult Stem Cells and Nanomaterials for Skeletal Tissue Engineering and Regeneration (2009)

Understanding nanobiology and application of nanotechnology is important in regenerative medicine, since nanoscale materials are the basic functional subunits of cells and tissues. Stem/progenitor cells are a promising candidate cell type in tissue engineering and regeneration, because of their expandability and multi‐differentiation potential. A key requirement in tissue engineering is the three‐dimensionality of the "regenerate tissue", particularly for weight‐bearing musculoskeletal tissues. The challenges in skeletal tissue engineering and regeneration and the application of adult stem cells and nanomaterial scaffolds will be presented. The biology of human mesenchymal stem cells, e.g., proliferation vs differentiation, is intricately regulated by cell‐cell interactions, signaling by extracellular biofactors, and transcriptional and epigenetic events. Architectural and structure‐dependent cues provided by the matrix also guide cell‐based tissue morphogenesis. We have developed biomimetic, biodegradable nanofibrous biomaterial scaffolds for cell‐based tissue engineering. The fabrication and biological basis of the scale‐dependent bioactivities of nanofibrous scaffolds will be presented, as well as their application for cartilage tissue engineering, including articular cartilage and intervertebral disc. (Support: NIH AR Z01 41131)
An adaptive grouping scheme in ultra-dense IEEE 802.11ax network using buffer state report based two-stage mechanism (2019)

IEEE 802.11ax, which is an emerging WLAN standard, aims at providing highly efficient communication in ultra-dense wireless networks. However, due to a large number of stations (STAs) in the ultra-dense device deployment scenarios, the potentially high packet collision rate significantly decreases the communication efficiency of WLAN. In this paper, we propose an adaptive STA grouping scheme to overcome this dense network challenge in IEEE 802.11ax by using Buffer State Report (BSR) based Two-stage Mechanism (BTM). In order to achieve the optimal efficiency of BSR delivery, we analyze the functional relationship between STA number in group and Resource Unit (RU) efficiency. Based on this analysis results, an adaptive STA grouping algorithm with variable group size is proposed to achieve efficient grouping in BTM. The numerical results demonstrate that the proposed adaptive BTM grouping algorithm significantly improves the BSR delivery efficiency and the throughput of overall system and each STA in the ultra-dense wireless network.
Resilient Soils for Resilient Farms: An Integrative Approach to Assess, Promote, and Value Soil Health for Small- and Medium-Size Farms (2022)

Healthy soils are the foundation of our food system and support broad environmental goals, including improved water quality and carbon sequestration. Increasingly, we appreciate that aspects of soil health such as fertility for plants, suppression of disease, and cycling of nutrients are linked to the functional diversity of soil microbial communities. The impact of functional capacity is influenced by farm management, which varies with farm size. Small to medium-size farms, with gross sales of less than $250,00 per year, make up 89% of farms in the United States, despite often being ignored in policy decisions. They are often more integrated into local food systems than larger farms, have greater investment in long-term sustainability due to their tendency to be passed from farmers to children, and are more reliant on soil health than larger farms. We describe a research framework to advance understanding of the soil microbiome and its role in soil health, environmental outcomes, and agricultural sustainability on small and medium farms. Our perspective elevates four research priorities to link the benefits of healthy soil microbiomes from the microscale to the macroscale. We hope this approach will lead to practical applications to enable success in the context of ongoing environmental and economic challenges that affect the agricultural landscape.
Cancer-Associated Fibroblasts Promote Aggressive Gastric Cancer Phenotypes via Heat Shock Factor 1–Mediated Secretion of Extracellular Vesicles (2021)

Gastric cancer is the third most lethal cancer worldwide, and evaluation of the genomic status of gastric cancer cells has not translated into effective prognostic or therapeutic strategies. We therefore hypothesize that outcomes may depend on the tumor microenvironment (TME), in particular, cancer-associated fibroblasts (CAF). However, very little is known about the role of CAFs in gastric cancer. To address this, we mapped the transcriptional landscape of human gastric cancer stroma by microdissection and RNA sequencing of CAFs from patients with gastric cancer. A stromal gene signature was associated with poor disease outcome, and the transcription factor heat shock factor 1 (HSF1) regulated the signature. HSF1 upregulated inhibin subunit beta A and thrombospondin 2, which were secreted in CAF-derived extracellular vesicles to the TME to promote cancer. Together, our work provides the first transcriptional map of human gastric cancer stroma and highlights HSF1 and its transcriptional targets as potential diagnostic and therapeutic targets in the genomically stable tumor microenvironment. SIGNIFICANCE: This study shows how HSF1 regulates a stromal transcriptional program associated with aggressive gastric cancer and identifies multiple proteins within this program as candidates for therapeutic intervention. GRAPHICAL ABSTRACT: http://cancerres.aacrjournals.org/content/canres/81/7/1639/F1.large.jpg.
Specifically decreased collagen biosynthesis in scurvy dissociated from an effect on proline hydroxylation and correlated with body weight loss. In vitro studies in guinea pig calvarial bones. (1983)

The question whether ascorbate regulates collagen production solely through its direct role in proline hydroxylation was investigated. Proteins in calvarial bones from control and scorbutic weanling guinea pigs were labeled in short-term cultures with radioactive proline. Proteins were digested with purified bacterial collagenase to distinguish between effects on collagen polypeptide production and hydroxyproline formation. There was a preferential decrease in the absolute rate of collagen biosynthesis beginning after 2 wk of ascorbate deficiency, and this effect was temporally dissociated from decreased proline hydroxylation. There were no significant changes in the absolute rates of collagen degradation or noncollagen protein production. In vitro inhibition of proline hydroxylation in normal bone with alpha, alpha'-dipyridyl did not affect the relative rate of collagen synthesis, further dissociating these functions. Ascorbate added to scorbutic bone cultures reversed defective proline hydroxylation but not defective collagen synthesis, suggesting that the latter was an indirect effect of scurvy. There was a linear correlation between the extent of body weight lost during the 3rd and 4th wk of scurvy and the rate of collagen synthesis in scorbutic bone. This correlation also applied to control animals receiving ascorbate, but with weight loss induced by food restriction. These studies establish for the first time that ascorbate deficiency in guinea pigs leads to a specific decrease in collagen polypeptide synthesis and suggest that this decrease results from the reduced food intake and/or weight-loss characteristic of scurvy.
The Impact of Neurotechnology on Rehabilitation (2008)

This paper present results of a multi-disciplinary project that is developing a microchip-based neural prosthesis for the hippocampus, a region of the brain responsible for the formation of long-term memories. Damage to the hippocampus is frequently associated with epilepsy, stroke, and dementia (Alzheimer's disease) and is considered to underlie the memory deficits related to these neurological conditions. The essential goals of the multi-laboratory effort include: (1) experimental study of neuron and neural network function--how does the hippocampus encode information? (2) formulation of biologically realistic models of neural system dynamics--can that encoding process be described mathematically to realize a predictive model of how the hippocampus responds to any event? (3) microchip implementation of neural system models--can the mathematical model be realized as a set of electronic circuits to achieve parallel processing, rapid computational speed, and miniaturization? and (4) creation of hybrid neuron-silicon interfaces-can structural and functional connections between electronic devices and neural tissue be achieved for long-term, bi-directional communication with the brain? By integrating solutions to these component problems, we are realizing a microchip-based model of hippocampal nonlinear dynamics that can perform the same function as part of the hippocampus. Through bi-directional communication with other neural tissue that normally provides the inputs and outputs to/from a damaged hippocampal area, the biomimetic model could serve as a neural prosthesis. A proof-of-concept will be presented in which the CA3 region of the hippocampal slice is surgically removed and is replaced by a microchip model of CA3 nonlinear dynamics--the "hybrid" hippocampal circuit displays normal physiological properties. How the work in brain slices is being extended to behaving animals also will be described.