gebhart/patrepeval huggingface dataset:
A Confocal Microscopic Analysis of Galaninergic Hyperinnervation of Cholinergic Basal Forebrain Neurons in Alzheimer's Disease (1997)

The galanin (GAL) containing peptide fiber system innervates the basal forebrain and has been shown to hyperinnervate remaining cholinergic neurons in Alzheimer's disease (AD). GAL modulates the release of acetylcholine and, therefore, may depress this neurotransmitter in surviving cholinergic basal forebrain (CBF) neurons in AD. The aim of this study was to identify putative synaptic contacts between GAL immunoreactive processes and CBF neurons and evaluate whether these processes hypertrophy in AD patients. We observed by confocal laser microscopy a hyperinnervation of GAL-containing fibers in both AD and Parkinson's disease patients with concurrent AD (PD/AD). Galaninergic fibers were often seen in direct apposition to remaining CBF neurons and enwrapped cholinergic cell soma and dendrites. Our results demonstrate that GAL-containing fibers are in direct apposition to CBF neurons in normal-aged humans and that this phenotype is enhanced in AD and PD/AD, suggesting that direct synaptic contacts occur between GAL-containing fibers and CBF neurons. Because GAL can modulate acetylcholine release from cholinergic neurons, hyperinnervation of GAL fibers in AD and PD/AD patients may further decrease release of acetylcholine from remaining CBF neurons. We propose that therapies based solely on acetylcholinesterase inhibitors may be insufficient to effectively increase cortical levels of acetylcholine.
Noncontact measurement of body position and vital information using airborne ultrasound (2016)

Because of a growing need for unconstrained medical monitoring for unobtrusively observing individuals in a living space, we have been studied about non-contact measurement of vital information such as respiration and heartbeat using airborne ultrasound. In previous study, the measurement system of small displacement using the M-sequence-modulated signal and tracking phase difference of reflected signals from the target has been proposed. The measurement of respiration and heartbeat of the target in a standing position using a pair of the loudspeaker and the microphone has also been performed. However, body position cannot be measured using a pair of the loudspeaker and the microphone because the system can measure the distance to the body only. In this paper, we describe a basic study of the measurement of body position, respiration, and heartbeat. In the system, using microphone array, body position was estimated by synthetic aperture processing. In addition, small body-surface velocity by respiration and heartbeat were measured by tracking phase difference of reflected signals.
Design verification of the S3.mp cache-coherent shared-memory system (1998)

This paper describes the methods used to formulate and validate the memory subsystem of the cache-coherent Sun Scalable Shared-memory MultiProcessor (S3.mp) at three levels of abstraction: the memory consistency model, the cache coherence protocol, and the implementation.
[PCR value in the diagnosis of feto-placental human parvovirus B19 hydrops fetalis: apropos of 10 cases]. (1997)

Human parvovirus B19 primary infection during pregnancy is responsible for 27% of non autoimmune hydrops fetalis. Parvovirus B19 antigen detection and parvovirus B19 IgM and IgG antibody determination using enzyme immunoassays are not reliable for diagnostic purposes and lack of specificity. Parvovirus B19 DNA detection in amniotic fluid, fetal blood, ascitic fluid, and fetal biopsies or placenta specimens seems to be the best method for the diagnosis. Ninety-seven samples from 70 cases of spontaneous abortions after fetal death or hydrops fetalis were examined using PCR. A 270-bp length fragment of the NSI gene was amplified using PCR followed by electrophoresis, by Dot-blot hybridization assay using a biotinylated probe and by Southern-blot hybridization assay using a horseradish peroxidase-labelled probe followed by chemiluminescent assay. The Southern-blot hybridization assay was the longest test but the most sensitive. The parvovirus B19 genome was identified in 10 cases. In two cases, intrauterine blood transfusions led to the cessation of symptoms and to the birth of normal babies.
Waste Heat Recovery From Distributed Rack-based Fuel Cells Using Thermoelectric Generators (2018)

Off-grid power generation has been demonstrated in data centers through the deployment of site-specific centralized power plants utiLizing gas turbine or fuel cell-based power generation. Because power is centrally generated, power distribution requires a high voltage power grid within the data center with its ancillary storage and conditioning requirements and equipment. An alternative approach is a completely decentralized distributed power generation system in which fuel cells deployed within individual server racks provide power localized to that rack only. Among other advantages, such an approach also greatly increases the ability to modulate and control power to individual rack units. Because the Solid Oxide Fuel Cells (SOFC) proposed in this approach are air-cooled and have extremely high air exhaust temperatures, of order 800 °C, the optimal energy efficient design of an overall localized fuel-cell power generation system should also consider the opportunities to recover and re-use the waste heat. This paper reports on the development of a coupled thermal-electrical model of a thermoelectric generator (TEG) based energy recovery system operating between the fuel cell hot exhaust air temperature and a warm water cooling system deployed within the rack for server cooling. The power generation system consisted of a TEG module sandwiched between a hot air heat exchanger and a colder water based heat exchanger. The design of the TEG module for maximum power generation is heavily coupled with thermal and electrical conditions. Distribution of temperature on generator surfaces change the optimum design, hence the optimization of generator system required co-optimization with the design of the heat exchangers. This paper presents the results of electro-thermal co-optimization, which considers the impact of the flow rates and temperatures of exhaust gas and cooling water, parallel and counter-flow arrangements, as well as the TEG packaging design including junctions, fractional area coverage, and substrate thickness.
Bioorthogonal Reaction Pairs Enable Simultaneous, Selective, Multi‐Target Imaging (2011)

Wechselseitig orthogonale Tetrazin-trans-Cycloocten- und Azid-Cyclooctin-Cycloadditionen wurden simultan für das bioorthogonale Markieren zweier verschiedener Zellpopulationen in der gleichen Kultur genutzt. Diese niedermolekularen Molekülsonden zeigen eine gute chemische Reaktivität und können einfach in biologische Systeme eingebaut werden. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Evidence of cross-cutting and redox reaction in Khatyrka meteorite reveals metallic-Al minerals formed in outer space (2017)

We report on a fragment of the quasicrystal-bearing CV3 carbonaceous chondrite Khatyrka recovered from fine-grained, clay-rich sediments in the Koryak Mountains, Chukotka (Russia). We show higher melting-point silicate glass cross-cutting lower melting-point Al-Cu-Fe alloys, as well as unambiguous evidence of a reduction-oxidation reaction history between Al-Cu-Fe alloys and silicate melt. The redox reactions involve reduction of FeO and SiO<sub>2</sub> to Fe and Fe-Si metal, and oxidation of metallic Al to Al<sub>2</sub>O<sub>3</sub>, occurring where silicate melt was in contact with Al-Cu-Fe alloys. In the reaction zone, there are metallic Fe and Fe-Si beads, aluminous spinel rinds on the Al-Cu-Fe alloys, and Al<sub>2</sub>O<sub>3</sub> enrichment in the silicate melt surrounding the alloys. From this and other evidence, we demonstrate that Khatyrka must have experienced at least two distinct events: first, an event as early as 4.564 Ga in which the first Al-Cu-Fe alloys formed; and, second, a more recent impact-induced shock in space that led to transformations of and reactions between the alloys and the meteorite matrix. The new evidence firmly establishes that the Al-Cu-Fe alloys (including quasicrystals) formed in outer space in a complex, multi-stage process.
2.0 Å resolution structure of a ternary complex of pig muscle phosphoglycerate kinase containing 3-phospho-D-glycerate and the nucleotide Mn adenylylimidodiphosphate (1996)

The crystal structure of a ternary complex of pig muscle phosphoglycerate kinase (PGK) containing 3-phosphoglycerate (3-PG) and manganese adenylylimidodiphosphate (Mn AMP-PNP) has been determined and refined at 2.0 A resolution. The complex differs from the true substrate ternary complex only in the presence of an imido- rather than an oxylink between beta- and gamma-phosphates of the bound nucleotide. The 3-PG is bound in a similar manner to that observed in binary complexes. The nucleotide is bound in a similar manner to Mg ADP except that the metal ion is coordinated by all three alpha-, beta-, and gamma-phosphates, but not by the protein. The gamma-phosphate, which is transferred in the reaction, is not bound by the protein. One further characteristic of the ternary complex is that Arg-38 moves to a position where its guanidinium group makes a triple interaction with the N-terminal domain, the C-terminal domain, and the 1-carboxyl group of the bound 3-PG. Although a hinge-bending conformation change is seen in the ternary complex, it is no larger than that observed in the 3-PG binary complex. To reduce that distance between two bound substrates to a value consistent with the direct in-line transfer known to occur in PGK, we modeled the closure of a pronounced cleft in the protein structure situated between the bound substrates. This closure suggested a mechanism of catalysis that involves the "capture" of the gamma-phosphate by Arg-38 and the N-terminus of helix-14, which has a conserved Gly-Gly-Gly phosphate binding motif. We propose that nucleophilic attack by the 1-carboxyl group of the 3-PG on the gamma-phosphorus follows the capture of the gamma-phosphate, leading to a pentacoordinate transition state that may be stabilized by hydrogen bonds donated by the NH groups in the N-terminus of helix 14 and the guanidinium group of Arg-38. During the course of the reaction the metal ion is proposed to migrate to a position coordinating the alpha- and beta-phosphates and the carboxyl group of Asp-374. The mechanism is consistent with the structural information from binary and ternary substrate complexes and much solution data, and gives a major catalytic role to Arg-38, as indicated by site-directed mutagenesis.
Abstract 712: Antitumor activity of SPINK1 monoclonal antibody in a subset of SPINK1 positive ETS-negative prostate cancer (2010)

Abstract We have identified SPINK1 outlier expression in an aggressive subset of TMPRSS2:ETS-negative prostate cancers (10% of all prostate cancers) by outlier meta-analysis (meta-COPA). We have further validated the mutual exclusivity of SPINK1 expression and ETS fusion status, demonstrated that SPINK1 outlier expression can be detected non-invasively in urine, and is a useful prognostic marker in prostate cancer (Tomlins et al., 2008 and Laxman et al., 2008). Here we examined the in vitro and in vivo effects of SPINK1 outlier expression. We demonstrate that exogenous SPINK1 recombinant protein stimulate cell proliferation in benign and cancerous prostate cells. Benign prostate epithelial cells RWPE treated with SPINK1 recombinant protein or conditioned medium (CM) from 22RV1 cells (SPINK1+) showed dramatic increase in cell invasion in Boyden chamber Matrigel invasion assay, which was attenuated in the presence of an anti-SPINK1 monoclonal antibody (mAb). We also showed that invasion of 22RV1 cells is attenuated with SPINK1 mAb, while SPINK1 mAb has no effect on the invasion of PC3 cells (SPINK1−) invasion. SiRNA knockdown of SPINK1 in 22RV1 cells attenuated invasion, which could be rescued by recombinant SPINK1 protein or 22RV1 CM. Stable knockdown of SPINK1 in 22RV1 cells using shRNA resulted in decreased cell proliferation, cell invasion and motility. Moreover, stable knockdown of SPINK1 in 22RV1-Luc cells showed reduced tumor burden in an in vivo xenograft model. The preclinical in vivo efficacy of SPINK1 mAb was evaluated in SPINK1 positive 22RV1-Luc xenograft model established in BalbC nu. nu mice. Antitumor activities were determined from the analyses of tumor growth inhibition, defined as the decrease in the mean tumor volume for SPINK1 mAb treated mice versus IgG mAb treated mice. Twice a week intra-peritoneal administration of SPINK1 mAb (10mg/kg) resulted in a 65% reduction of tumor burden. This study provides the rationale for the development of humanized anti-SPINK1 monoclonal antibodies for clinical targeting of the subset of patients with SPINK1 positive and ETS-negative prostate cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 712.
Out-of-Order Sliding-Window Aggregation with Efficient Bulk Evictions and Insertions (Extended Version) (2023)

Sliding-window aggregation is a foundational stream processing primitive that efficiently summarizes recent data. The state-of-the-art algorithms for sliding-window aggregation are highly efficient when stream data items are evicted or inserted one at a time, even when some of the insertions occur out-of-order. However, real-world streams are often not only out-of-order but also burtsy, causing data items to be evicted or inserted in larger bulks. This paper introduces a new algorithm for sliding-window aggregation with bulk eviction and bulk insertion. For the special case of single insert and evict, our algorithm matches the theoretical complexity of the best previous out-of-order algorithms. For the case of bulk evict, our algorithm improves upon the theoretical complexity of the best previous algorithm for that case and also outperforms it in practice. For the case of bulk insert, there are no prior algorithms, and our algorithm improves upon the naive approach of emulating bulk insert with a loop over single inserts, both in theory and in practice. Overall, this paper makes high-performance algorithms for sliding window aggregation more broadly applicable by efficiently handling the ubiquitous cases of out-of-order data and bursts.
A novel fast-switching control for multiple pickup IPT systems (2013)

Multiple fast-switching pickup Inductive Power Transfer Systems are popular in clean rooms and other material handling applications. Currently these systems use power supplies rated at or above the sum of the total pickup rated power to prevent the system overloading. In consequence there is spare capacity which is unnecessary. This paper proposes a novel Dynamic Demand Control (DDC) method assuming the coupled loads are fast-switched and therefore continuously draw power when on. This enables power supplies with smaller capacity to be used without compromising system integrity and therefore saves system cost. The proposed control method requires minimal adjustments to the current system configuration and can therefore be easily implemented on existing systems. A design guide which helps predict the minimum power supply capacity that can be safely used for a given system is also proposed. Experimental results have shown that both the proposed control and the design guide are valid.
Design of CMOS Temperature Sensors Based on Ring Oscillators in 180-nm and 110-nm technology (2020)

In this work two on-chip smart temperature sensors are presented. Both designs are based on two ring oscillators operating complementary. The readout circuit is achieved using time-to-digital converter and digital counter. The first sensor is designed in 180-nm technology, and the other is designed in 110-nm. The simulation results from both sensors are presented in the temperature range from -40 to 125°C. The first design achieves an accuracy of +4.9/-0.2°C, while the other achieves an accuracy of ±3.8°C.
Location Coding by the Human Visual System: Multiple Topological Adaptations in a Case of Strabismic Amblyopia (2005)

Amblyopia, a major cause of vision loss, is a developmental disorder of visual perception commonly associated with strabismus (squint). Although defined by a reduction in visual acuity, severe distortions of perceived visual location are common in strabismic amblyopia. These distortions can help us understand the cortical coding of visual location and its development in normal vision, as well as in amblyopia. The history of retinotopic mapping in the visual cortex highlights the potential impact of amblyopia. Theories of amblyopia include topological disarray of receptors in primary visual cortex, undersampling from the amblyopic eye compared with normal eyes, and the presence of anomalous retinal correspondence or multiple cortical representations of the strabismic fovea. We examined the distortions in a strabismic amblyope, using a pop-out localization task, in which normal observers made errors dependent on the visual context of the stimulus. The localization errors of the strabismic amblyope were abnormal. We found that none of the available theories could fully explain this one patient's localization performance. Instead, the observed behavior suggests that multiple adaptations of the underlying cortical topology are possible simultaneously in different parts of the visual field.
Modeling Healthcare Processes Using Commitments: An Empirical Evaluation (2015)

The two primary objectives of this paper are: (a) to demonstrate how Comma, a business modeling methodology based on commitments, can be applied in healthcare process modeling, and (b) to evaluate the effectiveness of such an approach in producing healthcare process models. We apply the Comma approach on a breast cancer diagnosis process adapted from an HHS committee report, and presents the results of an empirical study that compares Comma with a traditional approach based on the HL7 Messaging Standard (Traditional-HL7). Our empirical study involved 47 subjects, and two phases. In the first phase, we partitioned the subjects into two approximately equal groups. We gave each group the same requirements based on a process scenario for breast cancer diagnosis. Members of one group first applied Traditional-HL7 and then Comma whereas members of the second group first applied Comma and then Traditional-HL7-each on the above-mentioned requirements. Thus, each subject produced two models, each model being a set of UML Sequence Diagrams. In the second phase, we repartitioned the subjects into two groups with approximately equal distributions from both original groups. We developed exemplar Traditional-HL7 and Comma models; we gave one repartitioned group our Traditional-HL7 model and the other repartitioned group our Comma model. We provided the same changed set of requirements to all subjects and asked them to modify the provided exemplar model to satisfy the new requirements. We assessed solutions produced by subjects in both phases with respect to measures of flexibility, time, difficulty, objective quality, and subjective quality. Our study found that Comma is superior to Traditional-HL7 in flexibility and objective quality as validated via Student's t-test to the 10% level of significance. Comma is a promising new approach for modeling healthcare processes. Further gains could be made through improved tooling and enhanced training of modeling personnel.
Effect of Purification on Pore Structure of HiPco Single-Walled Carbon Nanotube Aggregates (2002)

HiPco single-walled carbon nanotube (HPNT) containing Fe was purified by one-step purification with HCl-washing (D-method) and two-step purification with HCl-washing after air oxidation (GD-method). The N2 adsorption isotherm of HPNT at 77 K is of type II and it changes to type IV after purification. Purification by the D and GD methods increases the total surface area and micropore volume, and it decreases the average pore width from 3.5 nm to 1.7 and 1.0 nm, respectively.
Elucidating heavy/light chain pairing preferences to facilitate the assembly of bispecific IgG in single cells (2019)

Multiple strategies have been developed to facilitate the efficient production of bispecific IgG (BsIgG) in single host cells. For example, we previously demonstrated near quantitative (≥90%) formation of BsIgG of different species and isotypes by combining 'knob-into-hole' mutations for heavy chain heterodimerization with engineered antigen-binding fragments (Fabs) for preferential cognate heavy/light chain pairing. Surprisingly, in this study we found high yield (>65%) of BsIgG<sub>1</sub><i>without</i> Fab engineering to be a common occurrence, i.e., observed for 33 of the 99 different antibody pairs evaluated. Installing charge mutations at both C<sub>H</sub>1/C<sub>L</sub> interfaces was sufficient for near quantitative yield (>90%) of BsIgG<sub>1</sub> for most (9 of 11) antibody pairs tested with this inherent cognate chain pairing preference. Mechanistically, we demonstrate that a strong cognate pairing preference in one Fab arm can be sufficient for high BsIgG<sub>1</sub> yield. These observed chain pairing preferences are apparently driven by variable domain sequences and can result from a few specific residues in the complementarity-determining region (CDR) L3 and H3. Transfer of these CDR residues into other antibodies increased BsIgG<sub>1</sub> yield in most cases. Mutational analysis revealed that the disulfide bond between heavy and light chains did <i>not</i> affect the yield of BsIgG<sub>1</sub>. This study provides some mechanistic understanding of factors contributing to antibody heavy/light chain pairing preference and subsequently contributes to the efficient production of BsIgG in single host cells.
Fast analytical model of MZI micro-opto-mechanical pressure sensor (2018)

This paper presents a fast analytical procedure in order to design a micro-opto-mechanical pressure sensor (MOMPS) taking into account the mechanical nonlinearity and the optical losses. A realistic model of the photonic MZI is proposed, strongly coupled to a nonlinear mechanical model of the membrane. Based on the membrane dimensions, the residual stress, the position of the waveguide, the optical wavelength and the phase variation due to the opto-mechanical coupling, we derive an analytical model which allows us to predict the response of the total system. The effect of the nonlinearity and the losses on the total performance are carefully studied and measurements on fabricated devices are used to validate the model. Finally, a design procedure is proposed in order to realize fast design of this new type of pressure sensor.
An S Receptor Kinase Gene in Self-Compatible Brassica napus Has a 1-bp Deletion (1993)

Daphne R. Goring, Tracy L. Glavin, Ulrike Schafer, Steven J. Rothstein, An S Receptor Kinase Gene in Self-Compatible Brassica napus Has a 1-bp Deletion, The Plant Cell, Vol. 5, No. 5 (May, 1993), pp. 531-539