gebhart/patrepeval huggingface dataset:
WBP2 shares a common location in mouse spermatozoa with WBP2NL/PAWP and like its descendent is a candidate mouse oocyte activating factor (2018)

The sperm-borne oocyte-activating factor (SOAF) resides in the sperm perinuclear theca (PT). A consensus has been reached that SOAF most likely resides in the postacrosomal sheath (PAS), which is the first region of the PT to solubilize upon sperm-oocyte fusion. There are two SOAF candidates under consideration: PLCZ1 and WBP2NL. A mouse gene germline ablation of the latter showed that mice remain fertile with no observable phenotype despite the fact that a competitive inhibitor of WBP2NL, derived from its PPXY motif, blocks oocyte activation when coinjected with WBP2NL or spermatozoa. This suggested that the ortholog of WBP2NL, WBP2, containing the same domain and motifs associated with WBP2NL function, might compensate for its deficiency in oocyte activation. Our objectives were to examine whether WBP2 meets the developmental criteria established for SOAF and whether it has oocyte-activating potential. Immunoblotting detected WBP2 in mice testis and sperm and immunofluorescence localized WBP2 to the PAS and perforatorium of the PT. Immunohistochemistry of the testes revealed that WBP2 reactivity was highest in round spermatids and immunofluorescence detected WBP2 in the cytoplasmic lobe of elongating spermatids and colocalized it with the microtubular manchette during PT assembly. Microinjection of the recombinant forms of WBP2 and WBP2NL into metaphase II mouse oocytes resulted in comparable rates of oocyte activation. This study shows that WBP2 shares a similar testicular developmental pattern and location with WBP2NL and a shared ability to activate the oocyte, supporting its consideration as a mouse SOAF component that can compensate for a WBP2NL.
Identification of microRNA‐181 by genome‐wide screening as a critical player in EpCAM–positive hepatic cancer stem cells† (2009)

MicroRNAs (miRNAs) are endogenous small noncoding RNAs that regulate gene expression with functional links to tumorigenesis. Hepatocellular carcinoma (HCC) is the most common type of liver cancer, and it is heterogeneous in clinical outcomes and biological activities. Recently, we have identified a subset of highly invasive epithelial cell adhesion molecule (EpCAM)(+) HCC cells from alpha-fetoprotein (AFP)(+) tumors with cancer stem/progenitor cell features, that is, the abilities to self-renew, differentiate, and initiate aggressive tumors in vivo. Here, using a global microarray-based miRNA profiling approach followed by validation with quantitative reverse transcription polymerase chain reaction, we have demonstrated that conserved miR-181 family members were up-regulated in EpCAM(+)AFP(+) HCCs and in EpCAM(+) HCC cells isolated from AFP(+) tumors. Moreover, miR-181 family members were highly expressed in embryonic livers and in isolated hepatic stem cells. Importantly, inhibition of miR-181 led to a reduction in EpCAM(+) HCC cell quantity and tumor initiating ability, whereas exogenous miR-181 expression in HCC cells resulted in an enrichment of EpCAM(+) HCC cells. We have found that miR-181 could directly target hepatic transcriptional regulators of differentiation (for example, caudal type homeobox transcription factor 2 [CDX2] and GATA binding protein 6 [GATA6]) and an inhibitor of Wnt/beta-catenin signaling (nemo-like kinase [NLK]). Taken together, our results define a novel regulatory link between miR-181s and human EpCAM(+) liver cancer stem/progenitor cells and imply that molecular targeting of miR-181 may eradicate HCC.
Novel membranes for proton exchange membrane fuel cell operation above 120°C. Final report for period October 1, 1998 to December 31, 1999 (2000)

In this project we investigated the experimental performance of three new classes of membranes, composites of perfluorosulfonic acid polymers with heteropolyacides, hydrated oxides and fast proton conducting glasses, which are promising candidates as electrolytes for proton exchange membrane fuel cells (PEMFCs), capable of operation at temperatures above 120°C. The motivations for PEMFC's operation at this temperature are to: 1) minimize the CO poisoning problem (adsorption of CO onto the platinum catalyst is greatly reduced at these temperatures), 2) find better solutions for the water and thermal management problems in proton exchange membrane fuel cells, 3) find potentially lower cost materials for proton exchange membranes. We prepared and characterized a variety of novel membrane materials. The most promising of these have been evaluated for performance in a single, small area (5cm2) fuel cell run on hydrogen and oxygen. Our results establish the technical feasibility of PEMFC operation above 120°C.
Name matching between Chinese and Roman scripts (2009)

There are generally many ways to transliterate a name from one language script into another. The resulting ambiguity can make it very difficult to "untransliterate" a name by reverse engineering the process. In this paper, we present a highly successful cross-script name matching system that we developed by combining the creativity of human intuition with the power of machine learning. Our system determines whether a name in Roman script and a name in Chinese script match each other with an F-score of 96%. In addition, for name pairs that satisfy a computational test, the F-score is 98%.
Designing Interaction for Multi-agent Cooperative System in an Office Environment (2020)

Future intelligent system will involve very various types of artificial agents, such as mobile robots, smart home infrastructure or personal devices, which share data and collaborate with each other to execute certain tasks.Designing an efficient human-machine interface, which can support users to express needs to the system, supervise the collaboration progress of different entities and evaluate the result, will be challengeable. This paper presents the design and implementation of the human-machine interface of Intelligent Cyber-Physical system (ICPS),which is a multi-entity coordination system of robots and other smart devices in a working environment. ICPS gathers sensory data from entities and then receives users' command, then optimizes plans to utilize the capability of different entities to serve people. Using multi-model interaction methods, e.g. graphical interfaces, speech interaction, gestures and facial expressions, ICPS is able to receive inputs from users through different entities, keep users aware of the progress and accomplish the task efficiently
Characterization of Ultra-High-Q Si3N4 Micro-Ring Resonators with High-Precision Temperature Control (2018)

We observe ultra-high Q-factors in Si3N4 micro-ring resonators exceeding 2 × 106 at 850 nm. We characterize them with a novel method harnessing high-precision temperature scanning and determine their thermal tuning coefficient with an uncertainty of only 4 MHz/K.
An Expandable, Inducible Hemangioblast State Regulated by Fibroblast Growth Factor (2014)

During development, the hematopoietic and vascular lineages are thought to descend from common mesodermal progenitors called hemangioblasts. Here we identify six transcription factors, Gata2, Lmo2, Mycn, Pitx2, Sox17, and Tal1, that "trap" murine cells in a proliferative state and endow them with a hemangioblast potential. These "expandable" hemangioblasts (eHBs) are capable, once released from the control of the ectopic factors, to give rise to functional endothelial cells, multilineage hematopoietic cells, and smooth muscle cells. The eHBs can be derived from embryonic stem cells, from fetal liver cells, or poorly from fibroblasts. The eHBs reveal a central role for fibroblast growth factor, which not only promotes their expansion, but also facilitates their ability to give rise to endothelial cells and leukocytes, but not erythrocytes. This study serves as a demonstration that ephemeral progenitor states can be harnessed in vitro, enabling the creation of tractable progenitor cell lines.
109 Multimodal Endovascular Endoscopy in Carotid Atherosclerotic Disease (2016)

INTRODUCTION: Disruption of vulnerable atherosclerotic plaque is the precipitating factor that culminates in thrombosis and ischemia. Current technologies do not have sufficient power to identify subtle thrombogenic lesions (ie, ulcers) in the arterial endoluminal surfaces nor reliably predict markers for future symptomatology. We developed an ultra-thin scanning fiber endoscope (SFE) to directly visualize the endoluminal surface of carotid arteries and identify structural, chemical, and biological markers of vulnerable and complicated atherosclerotic plaques. METHODS: 1.2 mm and 2.1 mm endoscopes containing single optical fiber scanned by a piezoelectric drive mechanism were developed to illuminate tissue surfaces with red, blue, and green laser beams (RBG). Laser-induced backscattering (reflectance) and tissue fluorescence collected by a ring of optical fibers were then digitalized to reconstruct color videos with large fields of view. Then, SFE was studied ex vivo in human carotid arteries and in vivo in a rabbit model of atherosclerosis. After imaging, specimens were processed for histology; lesions were independently reviewed by 2 pathologists and matched to endoscopic images with analysis of variance. RESULTS: High-definition structural and chemical images of 20 carotid arteries harvested during hospital autopsies revealed 42 lesions. Based on anatomical features and tissue fluorescence, we developed a classifier that enabled identification of: healthy endovascular regions (smooth surfaces with strong homogeneous fluorescence); early plaques (dot or streak-like elevated lesions with low fluorescence); advance plaques (irregular elevated lesions with minimal to absent fluorescence); and complicated plaques (ulcers: punch-out dark-gray lesions with raised irregular bead-like borders and speckled fluorescence pattern; hematoma: absent reflection, black regions, and fluorescence). The image quality and validity of the classifier were reproduced in vivo in an atherogenic rabbit model. CONCLUSION: Multimodal SFE generates high-definition structural and label-free chemical images, and detects with high sensitivity and specificity small intravascular thrombi and subtle surface thrombogenic lesions in nonstenosing complicated plaques, even in cases not detected by conventional diagnostic modalities. Therefore, it holds the potential for becoming a new imaging platform in cerebrovascular and cardiovascular disease.
Magnetic and Mössbauer spectroscopy studies of nanocrystalline iron oxide aerogels (2006)

A sol-gel synthesis was used to produce iron oxide aerogels. These nanocrystalline aerogels have a pore-solid structure similar to silica aerogels but are composed entirely of iron oxides. Mössbauer experiments and x-ray diffraction showed that the as-prepared aerogel is an amorphous or poorly crystalline iron oxide, which crystallized as a partially oxidized magnetite during heating in argon. After further heat treatment in air, the nanocrystallites are fully converted to maghemite. The particles are superparamagnetic at high temperatures, but the magnetic properties are strongly influenced by magnetic interactions between the particles at lower temperatures.
Critical analysis of electrohysterographic methods for continuous monitoring of intrauterine pressure (2020)

Monitoring the progression of uterine activity provides important prognostic information during pregnancy and delivery. Currently, uterine activity monitoring relies on direct or indirect mechanical measurements of intrauterine pressure (IUP), which are unsuitable for continuous long-term observation. The electrohysterogram (EHG) provides a non-invasive alternative to the existing methods and is suitable for long-term ambulatory use. Several published state-of-the-art methods for EHG-based IUP estimation are here discussed, analyzed, optimized, and compared. By means of parameter space exploration, key parameters of the methods are evaluated for their relevance and optimal values. We have optimized all methods towards higher IUP estimation accuracy and lower computational complexity. Their accuracy was compared with the gold standard accuracy of internally measured IUP. Their computational complexity was compared based on the required number of multiplications per second (MPS). Significant reductions in computational complexity have been obtained for all published algorithms, while improving IUP estimation accuracy. A correlation coefficient of 0.72 can be obtained using fewer than 120 MPS. We conclude that long-term ambulatory monitoring of uterine activity is possible using EHG-based methods. Furthermore, the choice of a base method for IUP estimation is less important than the correct selection of electrode positions, filter parameters, and postprocessing methods. The presented review of state-of-the-art methods and applied optimizations show that long-term ambulatory IUP monitoring is feasible using EHG measurements.
1-(Dichloroiodo)-1H,1H-Perfluoroalkanes (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT1-(Dichloroiodo)-1H,1H-PerfluoroalkanesPierfrancesco Bravo, Vittorio Montanari, Giuseppe Resnati, and Darryl D. DesMarteauCite this: J. Org. Chem. 1994, 59, 20, 6093–6094Publication Date (Print):October 1, 1994Publication History Published online1 May 2002Published inissue 1 October 1994https://pubs.acs.org/doi/10.1021/jo00099a048https://doi.org/10.1021/jo00099a048research-articleACS PublicationsRequest reuse permissionsArticle Views329Altmetric-Citations23LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Access to care: the physician's perspective. (2011)

Private practice physicians in Hawaii were surveyed to better understand their impressions of different insurance plans and their willingness to care for patients with those plans. Physician experiences and perspectives were investigated in regard to reimbursement, formulary limitations, pre-authorizations, specialty referrals, responsiveness to problems, and patient knowledge of their plans. The willingness of physicians to accept new patients from specific insurance company programs clearly correlated with the difficulties and limitations physicians perceive in working with the companies (p<0.0012). Survey results indicate that providers in private practice were much more likely to accept University Health Alliance (UHA) and Hawaii Medical Services Association (HMSA) Commercial insurance than Aloha Care Advantage and Aloha Quest. This was likely related to the more favorable impressions of the services, payments, and lower administrative burden offered by those companies compared with others.
Self‐suppression of biofilm formation in the cyanobacterium S ynechococcus elongatus (2012)

Biofilms are consortia of bacteria that are held together by an extracellular matrix. Cyanobacterial biofilms, which are highly ubiquitous and inhabit diverse niches, are often associated with biological fouling and cause severe economic loss. Information on the molecular mechanisms underlying biofilm formation in cyanobacteria is scarce. We identified a mutant of the cyanobacterium Synechococcus elongatus, which unlike the wild type, developed biofilms. This biofilm-forming phenotype is caused by inactivation of homologues of type II secretion /type IV pilus assembly systems and is associated with impairment of protein secretion. The conditioned medium from a wild-type culture represses biofilm formation by the secretion-mutants. This suggested that the planktonic nature of the wild-type strain is a result of a self-suppression mechanism, which depends on the deposition of a factor to the extracellular milieu. We also identified two genes that are essential for biofilm formation. Transcript levels of these genes are elevated in the mutant compared with the wild type, and are initially decreased in mutant cells cultured in conditioned medium of wild-type cells. The particular niche conditions will determine whether the inhibitor will accumulate to effective levels and thus the described mechanism allows switching to a sessile mode of existence.
Genome-Scale Metabolic Model for the Green Alga Chlorella vulgaris UTEX 395 Accurately Predicts Phenotypes under Autotrophic, Heterotrophic, and Mixotrophic Growth Conditions (2016)

The green microalga Chlorella vulgaris has been widely recognized as a promising candidate for biofuel production due to its ability to store high lipid content and its natural metabolic versatility. Compartmentalized genome-scale metabolic models constructed from genome sequences enable quantitative insight into the transport and metabolism of compounds within a target organism. These metabolic models have long been utilized to generate optimized design strategies for an improved production process. Here, we describe the reconstruction, validation, and application of a genome-scale metabolic model for C. vulgaris UTEX 395, iCZ843. The reconstruction represents the most comprehensive model for any eukaryotic photosynthetic organism to date, based on the genome size and number of genes in the reconstruction. The highly curated model accurately predicts phenotypes under photoautotrophic, heterotrophic, and mixotrophic conditions. The model was validated against experimental data and lays the foundation for model-driven strain design and medium alteration to improve yield. Calculated flux distributions under different trophic conditions show that a number of key pathways are affected by nitrogen starvation conditions, including central carbon metabolism and amino acid, nucleotide, and pigment biosynthetic pathways. Furthermore, model prediction of growth rates under various medium compositions and subsequent experimental validation showed an increased growth rate with the addition of tryptophan and methionine.
Bioavailability of avocado‐derived mannoheptulose in dogs (2010)

Mannoheptulose (MH) is a 7‐carbon sugar found in avocados and other natural sources that acts to reduce glycolysis via hexokinase inhibition. It has been proposed as a calorie restriction (CR) mimetic that delivers anti‐aging and health‐promoting benefits of CR without reducing food intake. Three studies were conducted to evaluate MH bioavailability when fed to dogs as an avocado extract (AvX) based on MH levels in urine (Study 1) and plasma (Study 2 &amp; 3). In Study 1, Labrador Retrievers (LR; n=15) and Fox Terriers (n=15) were fed AvX‐containing diets formulated to deliver 0, 2 or 5 mg MH/kg BW. All dogs were subjected to 24‐hour quantitative urine collections. A dose‐dependent increase (p&lt;0.05) in urinary MH occurred with increasing dietary MH. In Study 2, LR (n=6) were fed AvX‐containing diets once daily to deliver 0, 1 or 2 mg MH/kg BW. Sequential blood samples were collected before and after feeding through 12 hr and at 24‐hr post‐feeding. Plasma MH increased (P&lt;0.05) with both MH diets compared to control. Peak MH occurred 6–8 hr post‐feeding and returned to non‐detectable levels by 24 hr. In Study 3, similar MH results were observed for LR (n=10) fed AvX‐containing diets twice daily to provide 0 or 2 mg MH/kg BW. Peak MH occurred within 2–4 hr of MH consumption and returned to non‐detectable levels by 24 hr. Mannoheptulose, fed as an avocado extract, is biologically available in dogs based on its appearance in plasma and urine.
Weak Convergence and Generalized Stability for Solutions to Random Dynamical Systems (1992)

Let (Ω, P) be a probability space. Consider the random differential equation (see Arnold and Kliemann [AK] and Crauel [C] for this terminology, and background) 1.1 $$ \dot x(t,\omega ) = {\text{ }}F(t,\omega ,x(t,\omega )),{\text{ }} - \infty < t < \infty , $$ where F(t, ω, ·) : ℝ d → ℝ d is a Lipschitz function for each t, ω, measurable on ℝ × Ω and is stationary: that is, there exists a measure-preserving flow τ t : Ω → Ω, −∞ < t < ∞ such that $$ F(t,\omega , \cdot ) = F(0,\tau _t (\omega ), \cdot ).$$
Quantitative PET reporter gene imaging of CD8+ T cells specific for a melanoma-expressed self-antigen (2008)

Adoptive transfer (AT) T-cell therapy provides significant clinical benefits in patients with advanced melanoma. However, approaches to non-invasively visualize the persistence of transferred T cells are lacking. We examined whether positron emission tomography (PET) can monitor the distribution of self-antigen-specific T cells engineered to express an herpes simplex virus 1 thymidine kinase (sr39tk) PET reporter gene. Micro-PET imaging using the sr39tk-specific substrate 9-[4-[(18)F]fluoro-3-(hydroxymethyl)-butyl]guanine ([(18)F]FHBG) enabled the detection of transplanted T cells in secondary lymphoid organs of recipient mice over a 3-week period. Tumor responses could be predicted as early as 3 days following AT when a >25-fold increase of micro-PET signal in the spleen and 2-fold increase in lymph nodes (LNs) were observed in mice receiving combined immunotherapy versus control mice. The lower limit of detection was approximately 7 x 10(5) T cells in the spleen and 1 x 10(4) T cells in LNs. Quantification of transplanted T cells in the tumor was hampered by the sr39tk-independent trapping of [(18)F]FHBG within the tumor architecture. These data support the feasibility of using PET to visualize the expansion, homing and persistence of transferred T cells. PET may have significant clinical utility by providing the means to quantify anti-tumor T cells throughout the body and provide early correlates for treatment efficacy.