gebhart/patrepeval huggingface dataset:
Harnessing a Novel P450 Fatty Acid Decarboxylase from Macrococcus caseolyticus for Microbial Biosynthesis of Odd Chain Terminal Alkenes (2018)

ABSTRACT Alkenes are industrially important platform chemicals with broad applications. In this study, we report a microbial conversion route for direct biosynthesis of medium and long chain terminal alkenes from fermentable sugars by harnessing a novel P450 fatty acid (FA) decarboxylase from Macrococcus caseolyticus (OleT MC ). We first characterized OleT MC and demonstrated its in vitro H 2 O 2 -independent activities towards linear and saturated C10:0-C18:0 FAs, with the highest activity for C16:0 and C18:0 FAs. Combining protein homology modeling, in silico residue mutation analysis, and docking simulation with direct experimental evidence, we elucidated the underlying mechanism for governing the observed substrate preference of OleT MC , which depends on the size of FA binding pocket, not the catalytic site. Next, we engineered the terminal alkene biosynthesis pathway, consisting of an engineered E. coli thioesterase (TesA*) and OleT MC , and introduced this pathway into E. coli for direct terminal alkene biosynthesis from glucose. The recombinant strain E. coli EcNN101 produced a total of 17.78 ± 0.63 mg/L odd-chain terminal alkenes, comprising of 0.9% ± 0.5% C11 alkene, 12.7% ± 2.2% C13 alkene, 82.7% ± 1.7% C15 alkene, and 3.7% ± 0.8% C17 alkene, and a yield of 0.87 ± 0.03 (mg/g) on glucose after 48 h in baffled shake flasks. To improve the terminal alkene production, we identified and overcame the electron transfer limitation in OleT MC , by introducing a two-component redox system, consisting of a putidaredoxin reductase CamA and a putidaredoxin CamB from Pseudomonas putida, into EcNN101, and demonstrated the terminal alkene production increased ∼2.8 fold after 48 h. Overall, this study provides a better understanding of the function of P450 FA decarboxylases and helps guide future protein and metabolic engineering for enhanced microbial production of target designer alkenes in a recombinant host.
Experimental Validation of Time Reversal Multiple Access for UWB Wireless Communications Centered at the 273 GHz Frequency (2022)

Ultra-high data rates with low-power consumption wireless communications and low-complexity receivers is one of the key requirements for the next 6-th Generation (6G) of communication networks. Sub-Terahertz (SubTHz) frequency bands can support Ultra-WideBand (UWB) communications and can thus offer unprecedented increase in the wireless network capacity, rendering those bands and relevant research a strong candidate technology for 6G. However, in contrast to millimeter-wave communications, the technological advances in subTHz transceivers are not yet completely mature. In this paper, we focus on the Time Reversal (TR) precoding scheme, which is a computationally simple and robust technique capable of focusing UWB waveforms on both time and space, by exploiting channel diversity when available. We design a novel experimental setup, including a THz link inside a waveguide and operating at the carrier frequency 273.6 GHz with 2 GHz transmission bandwidth, and deploy it to perform multi-user communications with simple modulation. Our results demonstrate large communication rates with only 3 mm space separation of the receiving antennas.
Ordered Structures in Unstrained, Epitaxial Ge−Si−C Films (1998)

An ordered GeSi2 structure has been observed in layers of approximate composition GeSi2Cx grown epitaxially on Si. Except for regions very close to the interface, the ordered phase forms throughout the layer and is readily identified by high-resolution electron microscopy and electron diffraction. The structure is formed by Ge−Si−Si ordering along the diamond [111] direction to yield a new structure with P3̄m1 symmetry. Analogous (but not as extensive) ordering in the Ge2SiCx (x = 6%) system is also observed. The role of carbon, which is incorporated into the material as Si4C tetrahedra, is used to explain the formation of these novel structures.
Progress in high power SiC microwave MESFETs (2003)

SiC MESFET's have shown an RF power density of 4.6 W/mm at 3.5 GHz and a power added efficiency of 60% with 3 W/mm at 800 MHz, demonstrating that SiC devices are capable of very high power densities and high efficiencies. Single devices with 48 mm of gate periphery were mounted in a hybrid circuit and achieved a maximum RF power of 80 watts CW at 3.1 GHz with 38% PAE.
Pseudocoordinación del tipo TOMAR Y en eurasia: 50 años después [pseudocoordination with TAKE AND in eurasia: 50 years later] (2017)

In 1966, Eugenio Coseriu published his article "Tomo y me voy" that dealt with the construction [‘take and’ + verb], which expresses the onset of an action, often with a sense of surprise or disapproval. He gathered examples from about 30 languages from a variety of sources and inspired future research (by Morreale, Kiparsky, Fehling, Larsson, Vannebo, García Sánchez and others). This presentation is a more comprehensive view in order to complete the work he began and also with the goal of exploring the origins of the phrase. I present data from more than sixty languages – twice as many as originally identified by Coseriu – all across Europe (with the main exception being Celtic), including Indo-European, Finno-Ugric, and Turkic languages as well as others, such as Basque and Maltese. The results of this study suggest that a single origin in Biblical Greek, as proposed by Coseriu, seems unlikely, and that although this construction has clearly spread extensively via contact, it may have independent origins in two or more clusters. (Presented in Spanish)
Divergent androgen regulation of unfolded protein response pathways drives prostate cancer (2015)

The unfolded protein response (UPR) is a homeostatic mechanism to maintain endoplasmic reticulum (ER) function. The UPR is activated by various physiological conditions as well as in disease states, such as cancer. As androgens regulate secretion and development of the normal prostate and drive prostate cancer (PCa) growth, they may affect UPR pathways. Here, we show that the canonical UPR pathways are directly and divergently regulated by androgens in PCa cells, through the androgen receptor (AR), which is critical for PCa survival. AR bound to gene regulatory sites and activated the IRE1α branch, but simultaneously inhibited PERK signaling. Inhibition of the IRE1α arm profoundly reduced PCa cell growth in vitro as well as tumor formation in preclinical models of PCa in vivo. Consistently, AR and UPR gene expression were correlated in human PCa, and spliced XBP-1 expression was significantly upregulated in cancer compared with normal prostate. These data establish a genetic switch orchestrated by AR that divergently regulates the UPR pathways and suggest that targeting IRE1α signaling may have therapeutic utility in PCa.
ChemInform Abstract: An Enantioselective Method for Reductive Alkylation of Aromatic Carboxylic Acid Derivatives. Examination of the Factors that Provide Stereoselectivity. (1989)

Abstract A detailed examination is given of the factors which control the stereoselectivity of alkylation of chiral amide enolates such as (III) or (IX) generated by Birch reduction of substrates such as (I) or (VIII).
13C-nuclear magnetic resonance (NMR) spectral studies on polysubstituted flavonoids. I. 13C-NMR spectra of flavones. (1980)

The 13C-NMR spectra of 61 flavone derivatives were measured in dimethyl sulfoxide. By consideration of the chemical shifts of skeletal carbons, the parameters (α and β values) and correction factor (ο value) necessary for calculating their carbon shifts were deduced. The skeletal carbons of polysubstituted flavones and flavonols could easily be assigned by the use of these values and SCS of OMe or OH. The 13C-NMR spectra were confirmed to give useful information that could not be obtained from other spectral data. The data were applied for the structure elucidation of unknown polysubstituted flavones.
Multinational comparative cross-sectional survey of views of medical students about acceptable terminology and subgroups in schizophrenia (2018)

The study received ethical approval from ERGO (Ethics and Research Governance Online; ID: 15972) and subsequently from the ethics committee at each site.
MicroRNA-340-5p inhibits colon cancer cell migration via targeting of RhoA (2020)

Colon cancer is the third most common cancer and a significant cause of cancer-related deaths worldwide. Metastasis is the most insidious aspect of cancer progression. Convincing data suggest that microRNAs (miRs) play a key function in colon cancer biology. We examined the role of miR-340-5p in regulating RhoA expression as well as cell migration and invasion in colon cancer cells. Levels of miR-340-5p and RhoA mRNA varied inversely in serum-free and serum-grown HT-29 and AZ-97 colon cancer cells. It was found transfection with miR-340-5p not only decreased expression of RhoA mRNA and protein levels in HT-29 cells but also reduced colon cancer cell migration and invasion. Bioinformatics analysis predicted one putative binding sites at the 3'-UTR of RhoA mRNA. Targeting this binding site with a specific blocker reversed mimic miR-340-5p-induced inhibition of RhoA activation and colon cancer cell migration and invasion. These novel results suggest that miR-340-5p is an important regulator of colon cancer cell motility via targeting of RhoA and further experiments are warranted to evaluate the role of miR-340-5p in colon cancer metastasis.
Preface (2006)

During the last four decades, technical progress in the field of light-emitting diodes (LEDs) has been breathtaking. State-of-the art LEDs are small, rugged, reliable, bright, and efficient. At this time, the success story of LEDs still is in full progress. Great technological advances are continuously being made and, as a result, LEDs play an increasingly important role in a myriad of applications. In contrast to many other light sources, LEDs have the potential of converting electricity to light with near-unit efficiency.
Three-dimensional imaging by self-reference digital holograms (2015)

Digital holography can serve as an effective tool for imaging three-dimensional scenes or objects, quickly and reliably. In this paper, recently developed methods of self-reference single channel digital holography are reviewed: Fresnel incoherent correlation holography (FINCH), Fourier incoherent single channel holography (FISCH), and the joined object reference digital interferometer (JORDI).
Brasilinolide A, a New Macrolide Antibiotic Produced by Nocardia brasiliensis: Producing Strain, Isolation and Biological Activity. (1997)

A new 32-membered macrolide antibiotic, brasilinolide A was isolated from the fermentation broth of Nocardia sp. IFM 0406. The producer was identified as Nocardia brasiliensis. The antibiotic was only active against Aspergillus niger, but not active against other fungi including yeasts as well as other filamentous like fungi and bacteria. Brasilinolide A exerted an immunosuppressive activity in the assay system of a mixed lymphocyte reaction (MLR).
VOLTTRON Lite: Integration Platform for the Transactional Network (2013)

In FY13, Pacific Northwest National Laboratory (PNNL) with funding from the Department of Energy’s (DOE’s) Building Technologies Office (BTO) designed, prototyped and tested a transactional network platform. The platform is intended to support energy, operational and financial transactions between any networked entities (equipment, organizations, buildings, grid, etc.). Initially, in FY13, the concept demonstrated transactions between packaged rooftop units (RTUs) and the electric grid using applications or “agents” that reside on the platform, on the equipment, on local building controller or in the Cloud. This document describes the core of the transactional network platform, the Volttron Lite™ software and associated services hosted on the platform. Future enhancements are also discussed. The appendix of the document provides examples of how to use the various services hosted on the platform.
Balloon Valvuloplasty for Aortic Stenosis (1990)

Balloon valvuloplasty has recently emerged as an option for elderly patients who are poor surgical candidates. With medical management the prognosis is dismal; definitive treatment is valve replacement. The new procedure provides hemodynamic and symptomatic relief, but there are major complications. Restenosis is common and mortality during follow-up remains high.
An Efficient Paging Strategy Based on Paging Agents of Base Stations in Cellular Mobile Networks (2003)

We propose a new paging strategy to reduce paging cost by adding paging agents at base stations. When a mobile-terminated call occurs, the base stations look up the paging agents to determine if terminal paging is actually to be made. An analytical model based on a Markov chain is used to evaluate the performance of the proposed strategy. The numerical results show that the proposed strategy significantly reduces the paging cost compared with the simultaneous paging strategy.
Enhancing photovoltaics with broadband high-transparency glass using porosity-tuned multilayer silica nanoparticle anti-reflective coatings (2014)

Multilayer stacks of nanoparticle films with varying degrees of porosity were fabricated by sequentially spin coating and sintering silica/polystyrene mixtures. This increased the average transmittance of glass, giving the highest reported values for facile synthesized multilayer structures.
Fungal biofilm reactor improves the quality of a fusion protein GLA::GFP produced by Aspergillus oryzae (2015)

Fungal biofilm is known to promote the excretion of secondary metabolites, in accordance with solid-state related physiological mechanisms. In this work, the potentialities of fungal biofilm will be investigated in the context of the production of a Gla::GFP fusion protein by Aspergillus oryzae. Since the production of this protein is under the control of the promoter glaB, specifically induced in solid-state fermentation, biofilm mode of culture is expected to enhance the global productivity. However, we found that the glaB promoter is also activated in submerged bioreactor and the fusion protein production is higher in this mode of culture. This result is related to the high shear stress leading to biomass autolysis and leakage of intracellular fusion protein into the extracellular medium. Moreover, 2D-gel electrophoresis highlights preservation of the fusion protein integrity produced in biofilm conditions whereas proteolysis strongly affects fusion protein recovery in the submerged cultures performed at high stirring rate. Fungal biofilm reactor design was then further investigated and the scale-up potentialities were evaluated. Indeed, the specific design investigated in this work involves the use of metal structured packing exhibiting a high specific area and that can be easily expanded to large-scale bioprocessing conditions.
COMPLEXATION OF METHYLMERCURY(II) BY DL-SELENOMETHIONINE (1985)

Abstract The complexation of metal ions by methionine [Met] has attracted considerable interest.1–3 However, the coordination chemistry of selenomethionine [Se-Met] has received much less attention despite the fact that it is one of the few naturally occuring selenoaminoacids and a component of at least one bacterial selenoenzyme.4 It has been shown by 1H NMR spectroscopy1 that the prototypical ‘soft’ metal ion, méthylmercury(II), binds to Met above pH 7 via the amino group and binds to the thioether group at low pH. A single crystal X-ray diffraction study has confirmed amino coordination in a complex isolated from alkaline solution.5
Quantum Noise Radar: Assessing Quantum Correlations (2019)

We suggest overcoming the "Rayleigh catastrophe" and reaching superresolution for imaging with both spatially and temporally-correlated field of a superradiant quantum antenna. Considering far-field radiation of two interacting spontaneously emitting two-level systems, we show that for the measurement of the temporally-delayed second-order correlation function of the scattered field, the Fisher information does not tend to zero with diminishing the distance between a pair of scatterers even for non-sharp time-averaged detection. For position estimation of a larger number of scatterers, measurement of the time-delayed function is able to provide a considerable accuracy gain over the zero-delayed function. We show also that the superresolution with the considered quantum antenna can be achieved for both near-field imaging and estimating parameters of the antenna.
High‐Relaxivity Magnetic Resonance Imaging (MRI) Contrast Agent Based on Supramolecular Assembly between a Gadolinium Chelate, a Modified Dextran, and Poly‐β‐Cyclodextrin (2008)

Nanosized contrast agents have great potential in magnetic resonance molecular imaging applications for clinical diagnosis. This study proposes new nanoparticles spontaneously formed under mild conditions and composed of a noncovalent adduct between a gadolinium complex, a polymer of beta-cyclodextrin (pbetaCD: MW 1.5 x 10(6) g mol(-1)) and a dextran grafted with alkyl chains (MD). The formation of this supramolecular nanoassembly is based upon a "lock-and-key" recognition process in which the hydrophobic alkyl chains of MD and the adamantyl moieties of macrocyclic Gd(III) chelates are included in the cavities of pbetaCD. The large number of betaCDs contained in the pbetaCD resulted in the formation of 200 nm diameter nanoparticles, each entrapping 1.8 x 10(5) molecules of a low-molecular-weight Gd complex. This system, which exhibits a great relaxivity enhancement (48.4 mM(-1) s(-1), at 20 MHz and 37 degrees C) compared to the Gd(III) chelate itself (5.2 mM(-1) s(-1)), appears to be a promising strategy for the in vivo targeted delivery of Gd(III) complexes. The mechanisms of particle formation, conjugation strategies, and relaxometric characterizations in the field of contrast-enhanced magnetic resonance imaging are discussed.
Design of a fungal biofilm reactor for recombinant protein production from Aspergillus oryzae (2014)

Fungi are microorganisms exhibiting high secretive power of various metabolites and have the ability to perform post-translational modifications during protein synthesis. In the field of fermentation industry, they are ideal hosts for secondary metabolites and recombinant protein production. At the industrial-scale, equipments usually required for solid-state or submerged fermentation of filamentous fungi have demonstrated their limitations in terms of productivity, mass transfers or products recovery (1, 2). Recently, fungal biofilm reactors were designed to combine advantages from submerged and solid-state culture and reveal their usefulness for greater secondary metabolites production relative to submerged culture conditions (3). In our work, we propose the design of a fungal biofilm reactor for a recombinant protein production from an Aspergillus oryzae strain containing a GFP reporter gene system under the control of a promoter specifically induced in solid-state conditions. The fungal biofilm reactor is composed of a metal structured packing, having the function of inert support for biofilm growth, immerged or aspersed by a liquid medium. Whereas recombinant protein production is not significantly different at the flask-scale between submerged and biofilm conditions, productivity is higher in the submerged conditions at the bioreactor-scale. Presence of recombinant proteins entrapped in the biofilm matrix highlights a diffusion constraint and a lower mass transfer in our fungal biofilm reactor. However, persistence of a free liquid biomass of low viscosity and fungal biomass retention on the support are attractive for the implementation of a continuous process in our fungal biofilm reactor. Further studies will consider a 2-D proteomic comparison of the extracellular medium from fungal biofilm reactor and submerged culture conditions in order to better understand proteins secretion and identify over-expressed proteins in biofilm conditions.
Tunable Photonic Crystal Fiber Couplers With a Thermo-Responsive Liquid Crystal Resonator (2008)

We theoretically address the thermo-optical response of multicore photonic crystal fiber (PCF) couplers infiltrated with nematic liquid crystals (LCs). The proposed PCF coupler consists of two identical cores separated by a third one which acts as a liquid crystal resonator. With an appropriate choice of the design parameters associated with the liquid crystal core, phase matching at a single wavelength can be achieved, thus enabling thermo-tunable narrow-band resonant directional coupling between the input and the output cores. The verification of the proposed coupler design is ensured through an accurate PCF analysis based on finite element and beam propagation methods. The enhanced thermo-optical properties of LC-based PCF couplers are highly attractive for photo-thermal sensing applications.
Cellular Imaging and Surface Marker Labeling of Hematopoietic Cells Using Quantum Dot Bioconjugates (2006)

Semiconductor quantum dots (qdots) are emerging as a new class of fluorescent labels. The unique optical properties of qdots make them appealing in laboratory diagnosis; however, qdot-based probes remain to be developed and evaluated for clinical laboratory applications. In this study, 2 different approaches were employed to label hematopoietic cells with qdots. The first was based on a generalized intracellular delivery of qdots using qdot-transferrin conjugates through receptor-mediated endocytosis. Hematopoietic cells from umbilical cord blood or bone marrow were successfully labeled with qdot-transferrin in cell cultures. The fluorescence signal of qdot-transferrin was detected in the cytoplasmic location. The second approach was to use qdot-antibodies for labeling cell surface markers. The monoclonal antibodies to CD5, CD19, and CD45 surface antigens were conjugated to qdots with distinct emission spectra. The qdot-linked antibodies were shown to bind successfully to specific cell markers on lymphocytes. The signal obtained from the labeling of cells was detectable by using fluorescence microscopy and flow cytometry. The qdot signals were shown to be target specific, bright, and photo stable. The results of this study demonstrated the feasibility of using qdots for cell labeling and surface marker analysis of hematopoietic cells. Given the superior optical properties of qdots as compared to conventional fluorescence dyes, the qdot-based probe offers a promising tool for hematology analysis in clinical laboratories.
A Decision Support System for Design for Privacy (2011)

Privacy is receiving increased attention from both consumers, who are concerned about how they are being tracked and profiled, and regulators, who are introducing stronger penalties and encouragements for organizations to comply with legislation and to carry out Privacy Impact Assessments (PIAs). These concerns are strengthened as usage of internet services, cloud computing and social networking spread. Therefore companies have to take privacy requirements into account just as they previously had to do this for security. While security mechanisms are relatively mature, system and product developers are not often provided with concrete suggestions from a privacy angle. This can be a problem because developers do not usually possess privacy expertise. In this paper we argue that it would be useful to move beyond current best practice – where a set of searchable privacy guidelines may be provided to developers – to automated support to software developers in early phases of software development. Specifically, our proposal is a decision support system for design for privacy focused on privacy by policy, to be integrated into the development environment. We have implemented a proof of concept and are extending this work to incorporate state-of-the art consent mechanisms derived from the EnCoRe (Ensuring Consent and Revocation) project [1].