gebhart/patrepeval huggingface dataset:
Structural insights into gene repression by the orphan nuclear receptor SHP (2013)

Small heterodimer partner (SHP) is an orphan nuclear receptor that functions as a transcriptional repressor to regulate bile acid and cholesterol homeostasis. Although the precise mechanism whereby SHP represses transcription is not known, E1A-like inhibitor of differentiation (EID1) was isolated as a SHP-interacting protein and implicated in SHP repression. Here we present the crystal structure of SHP in complex with EID1, which reveals an unexpected EID1-binding site on SHP. Unlike the classical cofactor-binding site near the C-terminal helix H12, the EID1-binding site is located at the N terminus of the receptor, where EID1 mimics helix H1 of the nuclear receptor ligand-binding domain. The residues composing the SHP-EID1 interface are highly conserved. Their mutation diminishes SHP-EID1 interactions and affects SHP repressor activity. Together, these results provide important structural insights into SHP cofactor recruitment and repressor function and reveal a conserved protein interface that is likely to have broad implications for transcriptional repression by orphan nuclear receptors.
Data from Mechanisms and Effects of Loss of Human Leukocyte Antigen Class II Expression in Immune-Privileged Site-Associated B-Cell Lymphoma (2023)

&lt;div&gt;Abstract&lt;p&gt;&lt;b&gt;Purpose and Experimental Design:&lt;/b&gt; Loss of human leukocyte antigen (HLA) expression on tumor cells is frequent in diffuse large B-cell lymphoma (DLBCL) arising in immune-privileged sites, such as the testis and central nervous system, and is associated with small homozygous deletions of &lt;i&gt;HLA-DQ/HLA-DR&lt;/i&gt; and larger hemizygous deletions of the MHC region. To better understand the significance of down-regulation of HLA class II expression in relation to the homozygous and hemizygous deletions, we analyzed global gene expression patterns in a series of 26 testicular DLBCL after characterization of these deletions.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Results:&lt;/b&gt; Low levels of HLA-DR mRNA in whole testicular DLBCL samples were associated with a strong down-regulation of numerous immune-related genes specific for T cells, macrophages, antigen presentation and processing, lymphocyte activation, chemokines and chemokine receptors, and the complement system. The number of CD3&lt;sup&gt;+&lt;/sup&gt; tumor-infiltrating T cells was also significantly lower in low expressors of HLA-DR mRNA. Interestingly, hemizygous and homozygous deletions in the MHC region did not have any additional effect on global gene expression.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Conclusion:&lt;/b&gt; In conclusion, we found that loss of HLA class II mRNA expression in testicular DLBCL is associated with a significant change in global gene expression patterns. This effect is independent of the mechanism causing the down-regulation of HLA class II genes in the lymphoma cells.&lt;/p&gt;&lt;/div&gt;
Heat‐pipe transient model for space applications (1991)

A two‐dimensional model is developed for simulating heat pipes transient performance following changes in the input/rejection power or in the evaporator/condenser temperatures. The model employs the complete form of governing equations and momentum and energy jump conditions at the liquid‐vapor interface. Although the model is capable of handling both cylindrical and rectangular geometries, the results reported are for a circular heat pipe with liquid lithium as the working fluid. The model incorporates a variety of other working fluids, such as water, ammonia, potassium, sodium, and mercury, and offers combinations of isothermal, isoflux, convective and radiative heating/cooling conditions in the evaporator and condenser regions of the heat pipe. Results presented are for lithium heat pipes with exponential heating of the evaporator and isothermal cooling of the condenser.
Optimization of Aggregated EV Power in Residential Communities with Smart Homes (2020)

Electric vehicles (EVs) tend to increase peak power for residences in the evening when house owners return home and begin charging. The aggregated EV charging demand can cause a sudden rise in the peak power at the distribution system level, resulting in a “dragon curve” Such phenomenon, combined with the “duck curve” that is caused by high photovoltaic (PV) penetration in residential communities, requires fast ramping rates and expanded capabilities for local distribution transformers and main feeder cables provided by the utility. As a solution, a residential energy storage system (RESS) can store surplus PV generation during midday and use the stored energy to support the peak power demand in the evening. House owners benefit from this strategy by avoiding electricity sales to the grid at low rates and by reducing energy usage during high Time-of-Use (ToU) periods. In this paper, a community with smart homes that include PV systems, RESSs and EVs was modeled. The EV models were developed based on data from the National Travel Household Survey (NHTS). The EV charging and RESS operation were scheduled to reduce the daily utility charge. The entire power system worked as virtual power plant as it kept the aggregated power constant for a long period of time.
Computational studies of collisional processes in inertial electrostatic glow discharge fusion devices (2003)

Computational studies of the inertial electrostatic confinement (IEC) devices have yielded amazing results. An analytical model of charge exchange collisions in the IEC plasma has been developed to include ion time-of-flight and fusion neutron generation rates. Results from the model simulating 10 mA of D/sub 2//sup +/ ion current in a 30-cm diameter IEC device at 50 kV match the experimental results of 10/sup 6/ fusion neutrons per second. The model has also been used to find the effects of grid diameter on neutron yield and show that it scales as diameter/sup -0.4058/. This factor is very close to the experimental scaling factor of diameter/sup -0.4171/.
Generation and functional characterization of mice with a conditional BMP7 allele (2009)

Bone Morphogenetic Proteins (BMPs) play multiple and important roles in embryonic development as well as in homeostasis and tissue repair in the adult. Bmp7 has been implicated in developmental disorders and in a variety of diseases, but functional studies to elucidate its role so far have been hampered, since mice deficient in BMP7 die around or just after birth. To facilitate such studies, we generated mice in which the Bmp7 gene has been rendered conditional-null by flanking its first coding exon with loxP sites. To this end, we adapted the two-loxP site strategy to Bacterial Homologous Recombination to create a Bacterial Artificial Chromosome-based vector for direct targeting in mouse embryonic stem cells. Functional analysis showed that in vivo, the conditional-null Bmp7(flx/flx) mice are phenotypically wild type, whereas post Cre-mediated recombination, the resulting Bmp7(delta/delta) mice are phenotypically null. Thus, this study validates the usefulness of the Bmp7(flx/flx) mouse which in turn should empower in vivo studies aimed at elucidating the roles of Bmp7 in postnatal development, homeostasis and disease.
Novel Photocyclization of a Highly Phenylated β,γ-Unsaturated Ketone to a Cyclopropyl Ketone, Involving Benzoyl Group Migration (1966)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNovel Photocyclization of a Highly Phenylated β,γ-Unsaturated Ketone to a Cyclopropyl Ketone, Involving Benzoyl Group MigrationLinwood P. Tenney, David W. Boykin Jr., and Robert E. LutzCite this: J. Am. Chem. Soc. 1966, 88, 8, 1835–1836Publication Date (Print):April 1, 1966Publication History Published online1 May 2002Published inissue 1 April 1966https://pubs.acs.org/doi/10.1021/ja00960a059https://doi.org/10.1021/ja00960a059research-articleACS PublicationsRequest reuse permissionsArticle Views139Altmetric-Citations24LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Guidelines and Best Practices for Applying the ATHENA Interoperability Framwork to Support SME Participation in Digital Ecosystems. (2007)

Deliverable DA8.2, ATHENA Integrated Project n° 507849.
On the Electrical Characterization of Grain Boundaries in Multicrystalline Silicon (2011)

Impurity decoration of grain boundaries (GB) introduces deep levels and enhances their recombination activity. In this study we use the standard DLTS technique to investigate the influence of the GB structure on the electrical properties of mc-Si. The detected DLTS spectra have an opposite signal compared to the usually known majority carrier peaks of deep levels, but standard DLTS analysis can yield trap energies and capture cross sections. By comparing the properties between GB and intra-grain regions with similar minority carrier lifetime, it is considered that the DLTS signals might be due to traps generated in the extended defects present both at GBs and grains. It is observed that ∑3 GB has deep level traps at the same energy level as ∑9 GB, while the trap concentration in the former one is lower than the latter. Furthermore, a phosphorous gettering treatment can reduce the recombination activities both in the grains and at the GBs.
Doping Level Dependence of Transport Properties in InAsSb Quantum Wells (2010)

We investigated the transport properties of the Al0.1In0.9Sb/InAs0.1Sb0.9 quantum wells (QWs). Intentional doping of donors to the QW is effective to decrease the sheet resistance and suppress its temperature dependence because the carrier density is maintained to be relatively high in low temperature. Additionally, although there exists a low mobility region near the heterointerface of the QW and a high mobility region far from the interface in this system, the doped electrons contribute to keep high mobility in low temperature because the increase of electrons prevents the accumulation of all electrons in the low mobility region.
Control of High Pretilt Angle for Nematic Liquid Crystal on Homeotropic Alignment Layer by In-situ Photoalignment Method (2004)

Pretilt angle generation and liquid crystal (LC) aligning capabilities for a nematic (N) LC with obliquely polarized UV exposure on a homeotropic polyimide (PI) surface by an in-situ photoalignment method were studied. The high pretilt angle of 30θ for NLC with obliquely polarized UV exposure on the PI surface for 20 min can be achieved. The pretilt angle generated in NLC by the in-situ photoalignment method was higher than that generated by a conventional photoalignment method on a homeotropic alignment layer. Also, we suggest that the generation of pretilt angle in NLC with obliquely polarized UV exposure during imidization of the polymer for homeotropic alignment is a promising method to generate a high pretilt angle. Lastly, the good electro-optical (EO) characteristics of the photoaligned vertical alignment (VA)-liquid crystal display (LCD) by UV exposure on the PI surface for 10 min were observed.
<title>Minimally invasive treatment of benign prostatic hyperplasia with high-intensity focused ultrasound using the Sonablate system: an updated report of Phase III clinical studies conducted in the USA</title> (2002)

Phase III multicenter study was conducted in the USA under the FDA approved protocol, to establish the safety and efficacy of High Intensity Focused Ultrasound (HIFU) in the treatment of Benign Prostatic Hyperplasia (BPH) using the Sonablate? system. A total of sixty eight patients have been treated to date in the Phase III study. Transrectal probes capable of real-time ultrasound imaging of the prostate and well as delivering the HIFU energy to the selected region in the prostate gland were used. Probes with different focal lengths were used to treat different size of prostate glands. The patients were treated in one treatment session and were followed up to a period of one year following the treatment. The subjects underwent physical exam, Digital Rectal Examination (DRE), cystoscopy, Transrectal Ultrasound (TRUS), blood work, urine exam and were given standard questionnaires before treatment and during the follow up period. Under this protocol HIFU was found to be safe and effective in reducing the BPH related symptoms and improving the peak urinary flow rate with preservation of sexual function and no permanent side effects.
Low-linewidth and tunable single frequency 1x2 multimode-interferometer-Fabry-Perot laser diode (2016)

In this paper, we present a novel 1x2 multi-mode-interferometer-Fabry-Perot (MMI-FP) laser diode, which demonstrated tunable single frequency operation with more than 30dB side mode suppression ratio (SMSR) and a tuning range of 25nm in the C and L bands, as well as a 750 kHz linewidth. These lasers do not require material regrowth and high resolution gratings; resulting in a simpler process that can significantly increase the yield and reduce the cost.
New Ruthenium(II) Complexes Bearing N-Heterocyclic Carbenes (2002)

N-heterocyclic carbene complexes of ruthenium(II), [CpRu(L*)2Cl] (2) and [CpRu(CO)(L*)Cl] (3) (Cp = η5-C5H5; L* = 1,3-dicyclohexyl-imidazolin-2-ylidene), have been obtained in high yields by reaction of [CpRu(PR2R‘)2Cl] (R = R‘ = Ph, 1a; R = Ph, R‘ = 2-MeC6H4, 1b) and [CpRu(CO){PPh2(2-MeC6H4)}Cl] (1c), respectively, with the free carbene L*. The mixed dicarbene complex [CpRu(CPh2)(L*)Cl] (4) is prepared from [CpRu(CPh2){PPh2(2-MeC6H4)}Cl] (1d) and an equimolar amount of L*, whereas subsequent reaction of 1d with L* leads to formation of 2, along with tetraphenylethene. The reaction of [Cp*Ru(PPh3)2Cl] (1e) with L* gives the pentamethylcyclopentadienyl derivative [Cp*Ru(PPh3)(L*)Cl] (5) (Cp* = η5-C5Me5) by displacement of 1 equiv of PPh3. Complex 5 reacts in toluene with CO, pyridine (Py), and N2CHCO2Et, affording [Cp*Ru(CO)(L*)Cl] (6), [Cp*Ru(Py)(L*)Cl] (7), and the mixed dicarbene [Cp*Ru(CHCO2Et)(L*)Cl] (8), which were isolated in high yields. The molecular structure of complex 6 has been determined by an X-ray investigation, and the carbene−ruthenium distance clearly indicates a single bond (2.0951(18) Å). The N-heterocyclic carbene does not undergo substitution by other two-electron ligands.
Effects of insulin incubation on insulin binding, glucose transport, and insulin degradation by isolated rat adipocytes. Evidence for hormone-induced desensitization at the receptor and postreceptor level. (1980)

We have examined the effect of in vitro hyperinsulinemia on insulin binding, glucose transport, and insulin degradation in isolated rat adipocytes. When cells were incubated with insulin for 2 or 4 h at 37 degrees C, followed by washing in insulin-free buffer to remove extracellular and receptor-bound insulin, a time and dose-dependent decrease in insulin receptors was observed, which was accompanied by a reduced ability of cells to degrade insulin. Furthermore, the quantitatively predicted rightward shift in the insulin-glucose transport dose-response curve could be demonstrated. In addition to this reduction in insulin sensitivity, a striking decrease in maximal insulin-stimulated glucose transport was observed in the 4-h insulin-treated cells, indicating an abnormality distal to the insulin receptor. Thus, in vitro insulin-induced insulin resistance in adipocytes is caused by both receptor and postreceptor abnormalities. The post-receptor defect is most likely at the level of the glucose transport system per se because the insulinlike agents, spermine and antiinsulin receptor antibodies, also had a markedly reduced ability to stimulate glucose transport in 4-h insulin-treated cells. On the other hand, when cells were incubated with 100 ng/ml insulin for up to 4 h, after which time 2-deoxy glucose uptake was measured without removing buffer insulin or allowing receptor-bound insulin to dissociate, no decrease in maximal insulin-stimulated glucose transport was found. In conclusion, (a) insulin leads to a dose-dependent loss of insulin receptors in freshly isolated adipocytes accompanied by the predicted functional consequence of decreased receptors, i.e., a rightward shift in the insulin-glucose transport dose-response curve, (b) prolonged incubation with insulin causes a marked postreceptor defect in the glucose transport system, (c) maintenance of the activated state of the glucose transport system prevents the expression of the post-receptor defect, (d) the location of the postreceptor abnormality is most likely in the glucose transport system per se, and (e) insulin-induced receptor loss is accompanied by a decrease in insulin degradation.
Three-Dimensional Nanoscale Mapping of Porosity in Solution-Processed ITO Multilayer Thin Films for Patternable Transparent Electrodes (2019)

Indium tin oxide (ITO) films constitute components of many layered heterostructures used for emergent technologies beyond conventional optoelectronics. Compositional and morphological changes have a direct impact on the device’s performance. Hence control over the morphology with advanced multimodal characterization approaches is required to evaluate the devices. Herein multilayer ITO films deposited by spin-coating were quantified in nanoscale detail in three dimensions by combining results from depth-sensitive neutron reflectometry (NR), noncontact topographic AFM images, and cross-sectional SEM images. Films with a different number of deposited layers were visually transparent even though the topmost layer was as high as 60% porous, with porosity gradually decreasing as the number of the underneath sublayers increased. Surface and interfacial roughness through the total film and individual layer thickness were obtained. NR data also furnished quantitative depth information on the films’ chemical composition and layer-by-layer bulk density, which has never been obtained before, providing a way to monitor and ultimately control the sheet resistivity via the pore network. When the same formulation is used for inkjet printing patterns, the larger pores disappear, and the optical properties are improved to >90% transmittance at all visible wavelengths. All 5L films achieved sheet resistivities as low as 10–2 Ω cm and can therefore be used as patternable transparent electrodes for many devices including liquid crystal displays.
The process of tip wetting at the spray injection end (2019)

Gasoline direct injection engines mainly use multi-hole high-pressure injectors. To respect the current pollutant regulation (particle number and particle mass) and continue to decrease pollutant emissions in the future, it is of outmost importance to identify the various sources of carbon particles. In gasoline direct injection, tip wetting can generate a progressive tip sooting that can be a source of large number of particles especially in hot engine conditions. The different topics related to the tip wetting are investigated here without counterbore after the metering hole in order to have a direct access to the optical visualization. In this article, the different phases of the tip wetting are identified experimentally and phenomenological models are proposed.
A 3D bioprinted in situ conjugated‐co‐fabricated scaffold for potential bone tissue engineering applications (2018)

There is a demand for progressive approaches in bone tissue engineering to repair and regenerate bone defects resulting from trauma or disease. This investigation sought to engineer a single-step in situ conjugated polymeric scaffold employing 3D printing technology as an innovative fabricating tool. A polymeric scaffold was engineered in situ employing sodium alginate as a bio-ink which interacted with a poly(ethyleneimine) solution on bioprinting to form a polyelectrolyte complex through ionic bond formation. Silica gel was included in the bio-ink as temporal inorganic support component and for ultimate enhancement of osteoinduction. Characterization of the biorelevant properties of the scaffold was undertaken via Fourier Transform Infrared Spectroscopy, Differential Scanning Calorimetry and Thermogravimentric Analysis, X-Ray diffraction, Scanning Electron Microscopy, and biomechanical testing. The scaffold maintained its 3D architecture for the duration of the 28-day degradation investigation, while potentially permitting the infiltration of nutrients, growth factor, and cells evident by the increased solvent penetration into the scaffold observed via Magnetic Resonance Imaging studies. The scaffold porosity and pore size were found to be 60% and 360 µm, respectively. Biomechanical evaluation revealed a Young's modulus of 18.37 MPa highlighting that the scaffold in its current form possesses the mechanical capabilities for certain bone tissue engineering applications. This investigation provided highlighted the applicability of alginate-poly(ethyeneimine)/silica for 3D bioprinting as a scaffold which could possess potential as a bone tissue engineering scaffold. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1311-1321, 2018.
Immunoregulatory functions of paf-acether. IV. Enhancement of IL-1 production by muramyl dipeptide-stimulated monocytes. (1990)

paf-Acether (paf) is a phospholipid mediator of inflammation released from monocytes along with IL-1. In this study, we have examined the role of paf on IL-1 production by human monocytes. When paf from 1 nM to 5 microM, but not its precursor lyso paf, was added to monocytes in the presence of muramyl dipeptide (MDP) or LPS, a marked increase in IL-1 activity over the value with MDP alone was observed. In contrast, paf alone had minimal activity over the same dose range. Antibodies against rHu IL-1 alpha and rHu IL-1 beta neutralized the increased IL-1 activity. Interestingly, MDP that prompts monocytes to synthesize IL-1, induced the synthesis of paf, as well. Most of the paf produced remained cell-associated and always preceded IL-1 synthesis. When the paf receptor antagonist, L-652,731 was added to monocytes, it prevented the enhancement of IL-1 activity induced by exogenous paf. In contrast, L-652,731 had little effect on MDP-induced IL-1 synthesis in the absence of exogenous paf. This may indicate that there are alternative mechanisms involved in the sequences of events leading to IL-1 production. It is also conceivable that the paf receptor antagonist is not able to compete or inhibit endogenous paf as well as it does for exogenous paf. Nevertheless, exogenous paf in association with a second signal, modulates IL-1 production from human monocytes in a positive manner. This may constitute another means through which paf can modulate inflammatory and immune reactions.
Molecular identification of Aspergillus fumigatus complex from lung transplant recipients using multilocus sequencing analysis (MLSA) (2022)

<i>A. fumigatus</i> appears to be the primary causative agent of colonization or invasive aspergillosis among LTRs. No cryptic species were identified.