gebhart/patrepeval huggingface dataset:
Transient nuclear lamin A/C accretion aids in recovery from vapor nanobubble-induced permeabilisation of the plasma membrane (2022)

Vapor nanobubble (VNB) photoporation is a physical method for intracellular delivery that has gained significant interest in the past decade. It has successfully been used to introduce molecular cargo of diverse nature into different cell types with high throughput and minimal cytotoxicity. For translational purposes, it is important to understand whether and how photoporation affects cell homeostasis. To obtain a comprehensive view on the transcriptional rewiring that takes place after VNB photoporation, we performed a longitudinal shotgun RNA-sequencing experiment. Six hours after photoporation, we found a marked upregulation of LMNA transcripts as well as their protein products, the A-type lamins. At the same time point, we observed a significant increase in several heterochromatin marks, suggesting a global stiffening of the nucleus. These molecular features vanished 24 h after photoporation. Since VNB-induced chromatin condensation was prolonged in LMNA knockout cells, A-type lamins may be required for restoring the nucleus to its original state. Selective depletion of A-type lamins reduced cell viability after VNB photoporation, while pharmacological stimulation of LMNA transcription increased the percentage of successfully transfected cells that survived after photoporation. Therefore, our results suggest that cells respond to VNB photoporation by temporary upregulation of A-type lamins to facilitate their recovery.
T1‐ and T2‐weighted fast spin‐echo imaging of the brachial plexus and cervical spine with IDEAL water–fat separation (2006)

IDEAL-FSE is a promising method for T1W and T2W imaging of the brachial plexus and cervical spine.
LiBOB as Conductive Salt or Additive for Li-Ion Batteries (2008)

Abstract not Available.
Porous Silicon Nanoparticle Photosensitizers for Singlet Oxygen and Their Phototoxicity against Cancer Cells (2011)

Porous Si nanoparticles, prepared from electrochemically etched single crystal Si wafers, function as photosensitizers to generate (1)O(2) in ethanol and in aqueous media. The preparation conditions for the porous Si nanoparticles were optimized to maximize (1) the yield of material; (2) its quantum yield of (1)O(2) production; and (3) its in vitro degradation properties. The optimal formulation was determined to consist of nanoparticles 146 ± 7 nm in diameter, with nominal pore sizes of 12 ± 4 nm. The quantum yield for (1)O(2) production is 0.10 ± 0.02 in ethanol and 0.17 ± 0.01 in H(2)O. HeLa or NIH-3T3 cells treated with 100 μg/mL porous Si nanoparticles and exposed to 60 J/cm(2) white light (infrared filtered, 100 mW/cm(2) for 10 min) exhibit ∼45% cell death, while controls containing no nanoparticles show 10% or 25% cell death, respectively. The dark control experiment yields <10% cytotoxicity for either cell type.
Sciatic and Peroneal Nerve Injuries After Endovascular Ablation of Lower Extremity Varicosities (2014)

Varicose veins have traditionally been treated by surgical intervention. When performed in the lower limb, surgical vein stripping can potentially cause injury to the saphenous, sural, tibial, and peroneal nerves due to anatomic proximity. Newer, minimally invasive procedures, such as endovenous laser ablation and endovenous radiofrequency ablation, are more commonly used today. Although the potential for neural injury is greatly reduced, endovenous laser ablation and endovenous radiofrequency ablation have been documented to cause neural damage. Here, we report rare complications of 2 cases of varicosity endovascular ablation. One case involves ablation of the lesser saphenous vein and resulted in injury to the proximal common peroneal and tibial as well as distal sciatic nerves. The second case involves ablation of the vein of Giacomini that resulted in a common peroneal nerve injury. We stress the importance of preoperative anatomic mapping of the highly variable venous and neural systems in the area of ablation to minimize neural complications.
Singlet Oxygen-induced Activation of Akt/Protein Kinase B is Independent of Growth Factor Receptors¶ (2003)

Singlet oxygen (1O2)-induced cytotoxicity is believed to be responsible for responses to photodynamic therapy and for apoptosis of T helper cells after UV-A treatment. Other cytotoxic oxidants, such as hydrogen peroxide and peroxynitrite have been shown to stimulate cell survival signaling pathways in addition to causing cell death. Both these oxidants stimulate the Akt/protein kinase B survival signaling pathway through activation of membrane tyrosine kinase growth factor receptors. We evaluated the ability of 1O2 to activate the Akt/protein kinase B pathway in NIH 3T3 cells and examined potential activation pathways. Exposure of fibroblasts to 1O2 elicited a strong and sustained phosphorylation of Akt, which occurred concurrently with phosphorylation of p38 kinase, a proapoptotic signal. Inhibition of phosphatidylinositol-3-OH kinase (PI3-K) completely blocked Akt phosphorylation. Significantly, cell death induced by 1O2 was enhanced by inhibition of PI3-K, suggesting that activation of Akt by 1O2 may contribute to fibroblast survival under this form of oxidative stress. 1O2 treatment did not induce phosphorylation of platelet-derived growth factor receptor (PDGFR) or activate SH-PTP2, a substrate of growth factor receptors, suggesting that PDGFR was not activated. In addition, specific inhibition of PDGFR did not affect Akt phosphorylation elicited by 1O2. Activation of neither focal adhesion kinase (FAK) nor Ras protein, both of which mediate responses to reactive oxygen species, appeared to be pathways for the 1O2-induced activation of the PI3-K-Akt survival pathway. Thus, activation of Akt by 1O2 is mediated by PI3-K and contributes to a survival response that counteracts cell death after 1O2-induced injury. However, unlike the response to other oxidants, activation of the PI3-K-Akt by 1O2 does not involve activation of growth factor receptors, FAK or Ras protein.
Measurement of the Total ActiveB8Solar Neutrino Flux at the Sudbury Neutrino Observatory with Enhanced Neutral Current Sensitivity (2004)

The Sudbury Neutrino Observatory has precisely determined the total active (nu(x)) 8B solar neutrino flux without assumptions about the energy dependence of the nu(e) survival probability. The measurements were made with dissolved NaCl in heavy water to enhance the sensitivity and signature for neutral-current interactions. The flux is found to be 5.21 +/- 0.27(stat)+/-0.38(syst) x 10(6) cm(-2) s(-1), in agreement with previous measurements and standard solar models. A global analysis of these and other solar and reactor neutrino results yields Deltam(2)=7.1(+1.2)(-0.6) x 10(-5) eV(2) and theta=32.5(+2.4)(-2.3) degrees. Maximal mixing is rejected at the equivalent of 5.4 standard deviations.
Professional behavior at Society meetings (1986)

Journal Article Professional behavior at Society meetings Get access Dennis A. Tribble, Pharm.D. Dennis A. Tribble, Pharm.D. Director of Pharmacy Alexian Brothers Medical Center 800 West Biesterfield, Road Elk Grove Village, IL 60007 Search for other works by this author on: Oxford Academic Google Scholar American Journal of Hospital Pharmacy, Volume 43, Issue 3, 1 March 1986, Pages 586–588, https://doi.org/10.1093/ajhp/43.3.586 Published: 01 March 1986
ChemInform Abstract: Gold‐Catalyzed Annulation of 2‐Alkynyl Benzaldehydes with Vinyl Ethers: Synthesis of Dihydronaphthalene, Isochromene, and Bicyclo[2.2.2]octane Derivatives. (2013)

Abstract Various mechanisms of the very mild reactions of acyclic and cyclic vinyl ethers are discussed.
MEMS for wireless communications: from RF-MEMS components to RF-MEMS-SiP (2003)

Wireless communication has led to an explosive growth of emerging consumer and military applications of radio frequency (RF), microwave and millimeter wave circuits and systems. Future personal (hand-held) and ground communications systems as well as communications satellites necessitate the use of highly integrated RF front-ends, featuring small size, low weight, high performance and low cost. Continuing chip scaling has contributed to the extent that off-chip, bulky passive RF components, such as high-Q inductors, ceramic and SAW filters, varactor diodes and discrete PIN diode switches, have become limiting. Micro-machining or MEMS technology is now rapidly emerging as an enabling technology to yield a new generation of high-performance RF-MEMS passives to replace these off-chip passives in wireless communication (sub)systems. This paper reviews the progress in RF-MEMS from a device and integration perspective. The worldwide state-of-the-art of RF-MEMS devices including switches, variable capacitors, resonators and filters are described. Next, it is stipulated how integration of RF-MEMS passives with other passives (as inductors, LC filters, SAW devices, couplers and power dividers) and, active circuitry (ASICs, RFICs) can lead to the so-called RF-MEMS system-in-a-package (RF-MEMS-SiP) modules. The evolution of the RF-MEMS-SiP technology is illustrated using IMEC's microwave multi-layer thin-film MCM-D technology which today already serves as a technology platform for RF-SiP.
Abstract 2650: PI 3-Kinase inhibitors enhance the synthetic lethality of Parp inhibitors (2015)

Abstract Introduction: Clinical trials have shown promising responses of BRCA-linked breast and ovarian cancers to PARP inhibitor therapy, but remissions are often short-lived and incomplete. Frequent loss of INPP4B and PTEN leads to activation in the PI3K pathway in TNBC, making the PI3K pathway an attractive treatment target. Here we present mechanistic data that explain how PI3K- and PARP-inhibition enhance each other. Methods: We examined the effects of PI3K-inhibitors BKM120 or BYL719 and PARP-inhibitor Olaparib on the metabolism of HCC1937 cells (BRCA1 5382C mutation and homozygous deletion of PTEN and p53) measuring lactic acid production, via seahorse analysis, carbon metabolism using targeted Mass Spectrometry (LC-MS/MS) and DNA synthesis. PI3K- but not Akt- or SGK-inhibition induced a DNA damage response in this BRCA1 mutant cell line as well as in a mouse model of BRCA1-related breast cancer (K14-Cre BRCA1f/f p53f/f) mouse model. Summary: Metabolic profiling showed that PI3K-inhibition decreased flux through glycolysis and specifically through the non-oxidative pentose-phosphate pathway, the main source of ribose-5-phosphate required for the de novo synthesis of nucleotides in BRCA1-mutant breast cancer cells. Nucleotide shortage led to replication stress with the appearance of γH2AX, increased poly-ADP-ribosylation and an acute drop in DNA synthesis in cell cultures as well as in vivo. PI3K inhibition led to a reduced and error-prone S-phase due to a decrease in nucleotide biosynthesis which could be rescued upon exogenous supply of nucleosides. In a mouse model (K14-Cre BRCA1f/fp53f/f induced breast cancers) we could confirm that both BKM120 and BYL719 enhanced the efficacy of Parp-inhibitor Olaparib (median PFS for Olaparib 50 days, for BKM120 4 days, for BYL719 6 days and for both combinations, BKM120+Olaparib and BYL719+Olaparib &amp;gt; 120 days). Conclusion: Preceding cell cycle arrest, PI3K-inhibition leads to a decrease in DNA synthesis due to decreased Ribose-5-phosphate production required for nucleotide synthesis. PI3K-inhibitors lower nucleotide pools, lead to impaired DNA damage repair and S-phase progression and further augment the synthetic lethality of Parp-inhibitors in BRCA1-related breast cancer. Citation Format: Ashish P. Juvekar, Sina Yadegarynia, Hai Hu, Costas A. Lyssiotis, Hui Liu, John M. Asara, Ralph Scully, Lewis C. Cantley, Gerburg M. Wulf. PI 3-Kinase inhibitors enhance the synthetic lethality of Parp inhibitors. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 2650. doi:10.1158/1538-7445.AM2015-2650
FLOCCULATION DYNAMICS OF SYNTHETIC AND ACTIVATED SLUDGE IN WASTEWATER TREATMENT (2009)

This study has investigated in a comparative fashion the effect of cation, polysaccharide, and Polyelectrolyte on the flocculation dynamics and final properties of both synthetic and activated sludge. The results from experiments indicate that cation, polysaccharide, and Polyelectrolyte could influence sludge floc characteristics as they relate to sludge conditioning. The relationship between polysaccharide and cation concentration was examined at laboratory scale during flocculation with both synthetic and activated sludge; an increase in feed cation concentration led to a decrease in final polysaccharide concentration of the supernatant. The effects of a polyelectrolyte conditioner on sludge conditioning were determined. The results indicated that the cationic Polyelectrolyte had the critical effect on sludge dewatering, and made the improvement of the final properties of sludge. A qualitative link exists between polyelectrolyte conditioner dosage and sludge conditioning for both types of sludge. The two types of sludge also have very similar sludge conditioning. Thus synthetic and activated sludge behave very similarly in terms of their characteristics and sludge conditioning, and synthetic sludge can be used as a surrogate in activated sludge studies.
Spin valve device with sub-half micron core width fabricated by reactive etching (2005)

Summary form only given. The complete presentation was not made available for publication as part of the conference proceedings.
Image Theory Analysis of Fields Due to a Step in the Current in a Long Line on the Surface of the Earth (1984)

Calculations of the electric fields on the surface of uniform, two-layer,. and three-layer earths due to a line source excitation on the surface using complex image methods are reviewed. It is demonstrated that the analytical results can be readily interpreted using conventional plane wave reflection theory.
Effect of resonance on rare-earth ions in second-order nonlinear susceptibility recording in glasses (1996)

The chi<SUP>(2)</SUP>-grating recording in barium-borosilicate glasses doped with Tb or La ions was investigated. Correlation between BaO content and PSHG efficiency was established. The comparison of the absorption spectra with the data of PSHG efficiency measurements allows us to assume the role of resonance transition in Tb<SUP>3+</SUP> in the rising of PSHG effectiveness due to resonance increase of chi<SUP>(3)</SUP>.
Bias stress effect in low-voltage organic thin-film transistors (2008)

The bias stress effect in pentacene organic thin-film transistors has been investigated. The transistors utilize a thin gate dielectric based on an organic self-assembled monolayer and thus can be operated at low voltages. The bias stress-induced threshold voltage shift has been analyzed for different drain-source voltages. By fitting the time-dependent threshold voltage shift to a stretched exponential function, both the maximum (equilibrium) threshold voltage shift and the time constant of the threshold voltage shift were determined for each drain-source voltage. It was found that both the equilibrium threshold voltage shift and the time constant decrease significantly with increasing drain-source voltage. This suggests that when a drain-source voltage is applied to the transistor during gate bias stress, the tilting of the HOMO and LUMO bands along the channel creates a pathway for the fast release of trapped carriers.
Effect of Music on Stress Parameters in Dogs during a Mock Veterinary Visit (2022)

Veterinary visits can be stressful for dogs, but how their wellbeing changes during a visit is not well understood. Music therapy has been successfully used in clinical practice to alleviate stress and anxiety in people. The present study aimed to understand how canine stress changes during a veterinary visit, establish the effect of music, and highlight measures which may be of practical use. In a randomized crossover design, dogs were exposed to no music and a bespoke piece of classical music at a tempo designed to match their resting heart rate during a mock veterinary visit. Dogs were scored as more "afraid" during the physical examination compared to when they were in the hospital kennel (<i>p</i> < 0.001). Salivary cortisol, IgA, and infrared temperature all increased significantly (<i>p</i> < 0.05) from baseline to post-kennel and post-examination, with no effect of music treatment. Core body temperature (<i>p</i> = 0.010) and the odds of 'relaxed' lips (<i>p</i> = 0.020) were lower when dogs were exposed to music compared to control visits. Overall, dogs experienced changes in physiology and behavior, indicative of increased stress, over the course of the visit. Additional research is required to further understand the effect that bespoke music may have in alleviating canine stress during veterinary visits.
Structure–Activity Relationship Studies of Argiotoxins: Selective and Potent Inhibitors of Ionotropic Glutamate Receptors (2013)

Argiotoxin-636 (ArgTX-636), a natural product from the spider Argiope lobata, is a potent but nonselective open-channel blocker of ionotropic glutamate (iGlu) receptors. Here, three series of analogues were designed to exploit selectivity among iGlu receptors, taking advantage of a recently developed solid-phase synthetic methodology for the synthesis of ArgTX-636 and analogues. Initially, the importance of secondary amino groups in the polyamine chain was studied by the synthesis of systematically modified ArgTX-636 analogues, which were evaluated for pharmacological activity at NMDA and AMPA receptors. This led to the identification of two compounds with preference for NMDA and AMPA receptors, respectively. These were further elaborated by systematically changing the aromatic headgroup and linker amino acid leading to compounds with increased potency and selectivity for NMDA and AMPA receptors, respectively. Thus, the first structure-activity relationship study of ArgTX-636 has been carried out and has provided lead compounds for probing the ion channel region of iGlu receptors.
<title>Model-based HRR radar ATR: combining synthetic models with limited measured observations</title> (1999)

This paper addresses the use of synthetic data for air-to-air High Range Resolution (HRR) radar. Target radar models are used to generate synthetic HRR signatures in order to attempt to classify targets when there is limited real-life measured data. Target models are made up of a finite set of reflective patches. Modeling these targets is often difficult and frequently produces synthetic signatures which are not sufficiently close to measured data. We describe two approaches to improve classification of targets given limited measured HRR radar profiles and lower fidelity synthetic (model-based) profiles. The first method explores the possibility of improving model fidelity given measured HRR data. Specifically we search for material coating reflectance adjustments which consistently improve the synthetic predictions. The second approach attempts to predict missing measured data from available measured data based on global properties of the synthetic data. This is accomplished by using splines to interpolate and extrapolate between measured data and based on the global features in the synthetic data. The second method has the advantage that the model need only show global trends in the profiles over various viewing angles to aid in profile prediction. We also present algorithms for the alignment and normalization of measured data to support the above algorithms. Our results show that model corrections made by our algorithm demonstrate interpolative and extrapolative properties over regions where no measured data are available.
Detecting mechanical loosening of total hip replacement implant from plain radiograph using deep convolutional neural network (2019)

Plain radiography is widely used to detect mechanical loosening of total hip replacement (THR) implants. Currently, radiographs are assessed manually by medical professionals, which may be prone to poor inter and intra observer reliability and low accuracy. Furthermore, manual detection of mechanical loosening of THR implants requires experienced clinicians who might not always be readily available, potentially resulting in delayed diagnosis. In this study, we present a novel, fully automatic and interpretable approach to detect mechanical loosening of THR implants from plain radiographs using deep convolutional neural network (CNN). We trained a CNN on 40 patients anteroposterior hip x rays using five fold cross validation and compared its performance with a high volume board certified orthopaedic surgeon (AFC). To increase the confidence in the machine outcome, we also implemented saliency maps to visualize where the CNN looked at to make a diagnosis. CNN outperformed the orthopaedic surgeon in diagnosing mechanical loosening of THR implants achieving significantly higher sensitively (0.94) than the orthopaedic surgeon (0.53) with the same specificity (0.96). The saliency maps showed that the CNN looked at clinically relevant features to make a diagnosis. Such CNNs can be used for automatic radiologic assessment of mechanical loosening of THR implants to supplement the practitioners decision making process, increasing their diagnostic accuracy, and freeing them to engage in more patient centric care.
Charge Integrating Amplifier in the Near-Infrared Region Using an InGaAs PIN Photodiode (1993)

A low-noise detection system using an InGaAs PIN photodiode for near-infrared spectroscopic measurement has been developed. The InGaAs PIN photodiode is more suitable than a Ge PIN photodiode for detecting low-level light in terms of dark current and quantum efficiency. The detection system consists of an InGaAs PIN photodiode with a charge integrating amplifier (InGaAs-CIA) operated at 77 K. A minimum detectable power of 10 −16 W was achieved at a wavelength of 1.28 μm.
LPA as a Paracrine Mediator of Adipocyte Growth and Function (2000)

Adipogenesis corresponds to the recruitment of new adipocytes in adipose tissue, and results from the proliferation/differentiation of preadipocytes. Production of paracrine and autocrine factors by adipocytes plays an important role in adipogenesis. We recently demonstrated the existence of adipocyte production of lysophosphatidic acid (LPA) both in vitro and in situ. This production is modulated by catecholamines via alpha 2-adrenergic receptors. Adipocyte-LPA present in conditioned media increases the growth of a preadipose cell line in culture. This growth is associated with an activation of mitogen-activated protein kinases, and of the focal adhesion kinase. Because of the close proximity of preadipocytes and adipocytes within adipose tissue, adipocyte-LPA could play an important role in autocrine/paracrine control of adipogenesis.