gebhart/patrepeval huggingface dataset:
Molecular Technologies for Personalized Cancer Management (2004)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Positive and negative selection using the tetA-sacB cassette: recombineering and P1 transduction in Escherichia coli (2013)

The two-step process of selection and counter-selection is a standard way to enable genetic modification and engineering of bacterial genomes using homologous recombination methods. The tetA and sacB genes are contained in a DNA cassette and confer a novel dual counter-selection system. Expression of tetA confers bacterial resistance to tetracycline (Tc(R)) and also causes sensitivity to the lipophillic chelator fusaric acid; sacB causes sensitivity to sucrose. These two genes are introduced as a joint DNA cassette into Escherichia coli by selection for Tc(R). A medium containing both fusaric acid and sucrose has been developed, in which, coexpression of tetA-sacB is orders of magnitude more sensitive as a counter-selection agent than either gene alone. In conjunction with the homologous recombination methods of recombineering and P1 transduction, this powerful system has been used to select changes in the bacterial genome that cannot be directly detected by other counter-selection systems.
Tunable optical negative-index metamaterials employing anisotropic liquid crystals (2007)

A full-wave analysis technique based on the finite element-boundary integral method is developed and used to rigorously treat the scattering from periodically structured metamaterials incorporating anisotropic liquid crystals (LCs) and dispersive materials. Reconfiguration of the negative-index metamaterials is achieved by controlling the magnetic resonance via tuning permittivity of the embedded anisotropic LCs. Numerical results show that the refractive index of the metamaterials can be reconfigured by tuning the director orientation of anisotropic LCs or by using temperature-dependent LCs. The design configurations and their characteristics in the near- and the mid-infrared ranges are presented.
IA structures mediated by (2016)

Simian virus 40 chromatin was incubated after purification by sucrose density-gradient centrifugation with var- ious circular double-stranded DNA substrates. Monomeric rings were converted in the presence of Mg2+ to structures possessing a higher degree of complexity. Dimeric catenanes, as well as mul- timeric linear structures and concatemers, were generated, in- dicating that recombination events had occurred in vitro involving covalent linkage between different DNA molecules. Further- more, apparently fused dimeric rings were observed. Their struc- tures suggest that they may be recombination intermediates such as those described in a prokaryotic system (Potter, H. & Dressler, D. (1976) Proc. Natl. Acad. Sci. USA 73, 3000-3004). Recombi- nation did not take place between heterologous DNA substrates, as exclusively homologous multimeric DNA structures were observed.
Population dynamics of a recombinant culture in a chemostat under prolonged cultivation (1990)

The dynamics of a chemostat culture of Escherichia coli K12 harboring plasmid pBR322 under prolonged cultivation with a nonselective complex medium were studied. The ability of the culture to form colonies on plates supplemented with different ampicillin concentrations was monitored. It was observed that almost all cells sampled were able to grow on a high concentration of ampicillin at the beginning of the experiment. However, a subpopulation which formed colonies on intermediate-concentration (500-1000 mg/L) plates, but not on a high-concentration (2000 mg/L) plate, was detected just before the appearance of the plasmid-free cells. As time progressed, the percentage of this subpopulation increased, reached a maximum, then decreased toward the end of experiment. At this time the culture was dominated by a subpopulation which could not form colonies on the 100 mg/L ampicillin plates. These results indicate that three major processes may occur in the chemostat: a gradual shift of the higher plasmid copy number population toward a relatively lower copy number population; the complete shedding of the plasmid due to faulty segregation of plasmids during cell division; and growth competition among the subpopulations. A previously derived model is extended to account for all subpopulations. The model agrees qualitatively with the experimental results.
Use of fault dropping for multiple fault analysis (1994)

A new approach to fault analysis is presented. The authors consider multiple stuck-at-0/1 faults at the gate level. First, a fault collapsing phase is applied to the network, so that equivalent faults are eliminated. During the analysis the authors consider frontier faults where there is at least a normal path from each faulty line to a primary output. It is shown that the set of frontier faults is equivalent to the set of multiple faults. Given an input vector, the authors evaluate the fault-free circuit and then propagate fault effects. Assuming that fault-free response is observed, a fault-dropping procedure is then applied to eliminate faulty conditions on lines, that are either absent or may be hidden by other faulty conditions. This method is applied to some benchmark circuits and achieves a high degree of efficiency.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Effectiveness and safety of long-term treatment with sulfonylureas in patients with neonatal diabetes due to KCNJ11 mutations: an international cohort study (2018)

Wellcome Trust, Diabetes UK, Royal Society, European Research Council, Norwegian Research Council, Kristian Gerhard Jebsen Foundation, Western Norway Regional Health Authority, Southern and Eastern Norway Regional Health Authority, Italian Ministry of Health, Aide aux Jeunes Diabetiques, Societe Francophone du Diabete, Ipsen, Slovak Research and Development Agency, and Research and Development Operational Programme funded by the European Regional Development Fund.
Amino acid transporter LAT1 in tumor-associated vascular endothelium promotes angiogenesis by regulating cell proliferation and VEGF-A-dependent mTORC1 activation (2020)

These results demonstrate that the endothelial LAT1 is a novel key player in tumor angiogenesis, which regulates proliferation, translation, and pro-angiogenic VEGF-A signaling. This study furthermore indicates a new insight into the dual functioning of LAT1 in tumor progression both in tumor cells and stromal endothelium. Therapeutic inhibition of LAT1 may offer an ideal option to potentiate anti-angiogenic therapies.
Levels of translatable mRNAs for cell surface protein, collagen precursors, and two membrane proteins are altered in Rous sarcoma virus-transformed chick embryo fibroblasts. (1977)

Transformation of chick embryo fibroblasts by Rous sarcoma virus results in decreased amounts of a major cell surface protein and of collagen. To determine the mechanism accounting for the decreased production of these proteins, we have measured the relative amounts of functional mRNAs for these and other transformation-sensitive proteins. Total cellular RNAs extracted from normal cells and from cells transformed by the Schmidt-Ruppin strain of Rous sarcoma virus were translated in a cell-free system derived from wheat germ. Analysis of the in vitro translation products of RNAs from normal and transformed chick embryo fibroblasts shows a 5-fold reduction in the translatable mRNA for cell surface protein and a 10-fold reduction in translatable mRNA for two collagen precursors. In addition, increases in functional mRNA are observed for myosin and for two membrane polypeptides with molecular weights of 95,000 and 78,000; the latter two proteins increase on transformation, but the increases are in large part secondary to the depletion of glucose from the medium of transformed cells. Our data suggest that some of the major cellular changes induced by oncogenic viruses are due to changes in the activity of specific cellular genes.
Monodentate Phosphines Provide Highly Active Catalysts for Pd‐Catalyzed CN Bond‐Forming Reactions of Heteroaromatic Halides/Amines and (H)N‐Heterocycles (2006)

A good alternative: Highly reactive catalysts based on palladium and dialkylbiarylphosphino ligands provide unprecedented reactivity and selectivity in CN bond-forming processes. The bulky monophosphine catalyst system Pd/1 was effective for the reaction of aryl/heteroaryl halides bearing primary amides and 2-aminoheterocycles (see scheme; dba=dibenzylideneacetone, R=CONH2, NH2), thus showing that monodentate phosphines are viable alternatives to, and sometimes superior to, chelating ligands.
Colonic Structural and Ion Transport Abnormalities in Suckling Rabbits Infected with Escherichia coli K12 (1997)

Infection with E. coli K12 disrupts the epithelium and alters ion transport in the colon, probably as a result of mucosal inflammation. The changes indicate that nonpathogenic E. coli have the potential to cause intestinal disease.
Mechanisms for Post Processing Deformation without Part-Specific Tooling (2014)

Post processing deformation of additive manufactured components offers many advantages from reduced printing time and material usage to simplified multimaterial fabrication. For thermoplastic components, controlled deformations can be achieved by locally heating to temporarily soften the material. This paper investigates multiple methods of selectively heating and deforming desired locations to form the final component. The deformation location can be controlled by geometry (thickness), surface properties (reflectivity) or by local addition of a secondary material to control the heating. Demonstrations are provided for each of these deformation methods and then an example is provided to show how this approach could produce customized geometries from a standard feature.
Role of heat shock protein 60 in primed and naïve states of human pluripotent stem cells (2022)

Human pluripotent stem cells (hPSCs) exist in at least two distinct states in mammals: naïve pluripotency that represents several molecular characteristics in pre-implantation epiblast and primed pluripotency that corresponds to cells poised for differentiation in post-implantation epiblast. To identify and characterize the surface molecules that are necessary for the maintenance of naïve hPSCs, we generated a panel of murine monoclonal antibodies (MAbs) specific to the naïve state of hPSCs. Flow cytometry showed that N1-A4, one of the MAbs, bound to naïve hPSCs but not to primed hPSCs. Cell surface biotinylation and immunoprecipitation analysis identified that N1-A4 recognized heat shock protein 60 (HSP60) expressed on the surface of naïve hPSCs. Quantitative polymerase chain reaction (qPCR) analysis showed that HSP60 expression was rapidly downregulated during the embryoid body (EB) differentiation of primed hPSCs. HSP60 knockdown led to a decrease in the expression of pluripotency genes in primed hPSCs. HSP60 depletion also led to a decrease in the expression of pluripotency genes and representative naïve-state-specific genes in naïve hPSCs. Taken together, the results suggest that HSP60 is downregulated during differentiation of hPSCs and is required for the maintenance of pluripotency genes in both primed and naïve hPSCs, suggesting that HSP60 is a regulator of hPSC pluripotency and differentiation.
Improved Models for Interpreting the Pressure Response of Formation Testers (1991)

ABSTRACT New finite element models are presented in this paper which describe the pressure response of formation testers. The influences of formation anisotropy, bed thickness, and horizontal and dipping bed boundaries are investigated. The simulator utilizes techniques for grid optimization already used in improving the quality of finite element solutions for stress analysis of elastic solids and structures. Examples are given comparing interpretation results using the simulator against results obtained using prior standard models. Near-wellbore simulation (NEWS©) permits the investigator to introduce factors into the model in a manner consistent with basic principles of physics. A new analytic model is presented which incorporates some of the new observations from the finite element models, maintains space-dimensional consistency, and decouples the formation anisotropy and fluid compressibility relationship assumed in the development of the classical spherical flow model by Moran and Finklea.1 Observation points placed at strategic locations in the formation and around the wellbore allow investigation of the pressure response of formation testers with multiple-sensor capability. New techniques are presented for interpreting the pressure response of formation testers. NEWS© is a powerful tool for understanding and predicting the near-wellbore production dynamics of a well. It is anticipated that the simulations will lead to verification of new or existing models and will aid in developing improved tools and interpretation techniques.
OS1206 Investigation of functionally gradient of porous aluminum by X-ray CT observation and compressive test (2014)

Aluminum foam is a multifunctional material with light weight and high energy absorption. In this study, three-layered functionally graded ADC12 aluminum alloy foam with different pore structures was fabricated by varying amounts of blowing agent added and amounts of gases in ADC12 aluminum alloy die castings as starting materials. From X-ray computed tomography (X-ray CT) observations of the pore structures of fabricated three-layered functionally graded porous ADC12 aluminum, it was found that it has three layers with layer A (high porosity and large pore diameter), layer B (middle porosity and small pore diameter) and layer C (low porosity and small pore diameter). The deformation of the fabricated three-layered functionally graded porous ADC12 aluminum during compression tests started from the low strength layer A, and thereafter the middle strength layer B and high strength layer C sequentially deformed.
Hubris and narcissism: the dark underbelly of leadership (2009)

A certain degree of narcissism is perfectly natural and even healthy. A moderate measure of self-esteem contributes to positive behaviors such as assertiveness, confidence, and creativity, all desirable qualities for an individual in any walk of life, but particularly so for business leaders. At the other end of the spectrum, however, extreme narcissism is characterized by egotism, self-centeredness, grandiosity, lack of empathy, exploitation, exaggerated self-love, and failure to acknowledge boundaries. In this severe form, narcissism can do serious damage. This is especially true within an organization, where the combination of a leader's overly narcissistic disposition and his or her position of power can have devastating consequences.(Kets de Vries, 2006)
Central vein stenosis in a dialysis patient: a case report (2012)

Central vein stenosis is not a rare problem in patients on hemodialysis and can even occur without previous placement of central venous catheters. High shunt volumes seem to increase the risk associated with central vein catheters.
Differential intensity contrast swept source optical coherence tomography for human retinal vasculature visualization (2012)

We demonstrate an intensity-based motion sensitive method, called differential logarithmic intensity variance (DLOGIV), for 3D microvasculature imaging and foveal avascular zone (FAZ) visualization in the in vivo human retina using swept source optical coherence tomography (SS-OCT) at 1060 nm.A motion sensitive SS-OCT system was developed operating at 50,000 A-lines/s with 5.9 µm axial resolution, and used to collect 3D images over 4 mm 2 in a normal subject eye.Multiple B-scans were acquired at each individual slice through the retina and the variance of differences of logarithmic intensities as well as the differential phase variances (DPV) was calculated to identify regions of motion (microvasculature).En face DLOGIV image were capable of capturing the microvasculature through depth with an equal performance compared to the DPV.
Possibility for Probiotic Sources of Methionine for Organic Poultry Nutritional Supplementation: An Early Review (2013)

Methionine is a nutritionally essential amino acid required in the diet of humans and livestock, including poultry. Chickens are unable to produce methionine and therefore must obtain it through their diets. Generally, methionine is one of the first limiting amino acids in poultry nutrition and typically in most diets this amino acid has to be added to the poultry feed. Currently, methionine is produced by chemical processes or hydrolyzing proteins. However, chemical synthesis is expensive and produces a mixture of D- and L-methionine. In addition, these sources of amino acids are problematic as nutritional supplements for organic poultry production. It may be possible to develop microbial sources of methionine that would meet the criteria for organic use but since genetic modification is not allowed this will require isolation of naturally occurring methionine over-producers. Application of such cultures may work as external sources of pure methionine but it may be more cost effective to develop a probiotic approach either by directly administering such cultures or enriching for members of the gastrointestinal population already present that have this ability. This review discusses these strategies and the criteria required to meet the requirements for methionine supplementation in these production systems.
Role of academia, government and pharmaceutical industry in early-phase clinical trials of novel cancer immunotherapies (2005)

6061 Background: This study was initiated to assess the levels of involvement of Academia, Government and Industry in Phase I and II clinical trials of novel cancer immunotherapies. Methods: Two databases, ClinicalTrials.gov, maintained by National Institutes of Health (NIH), and Cancer.gov, maintained by the National Cancer Institute (NCI), were selected for review on the basis of the number of trials of novel immunotherapies, and the level of information provided. Over the month of July 2003, each database was extensively mined for all Pilot, Phase I, I/II and II trials listed as active, completed or suspended. There were 482 trial records gathered from Cancer.gov, and 600 trial records gathered from ClinicalTrials.gov. The datasets were combined, and duplicate studies were eliminated to yield a final, non-redundant dataset of 758 trials. Results: ClinicalTrials.gov identified Academia/Government collaborations as sponsor of 45% of trials, Government and Industry as sole sponsor in 36% and 10% of trials respectively. Cancer.gov showed a large difference in the roles of Academia and Government with Government sponsoring 75% of trials and Academia sponsoring 8% of trials. In contrast, Industry was the sole reagent provider in 82% of trials, and a co-provider in an additional 8% of trials. We were unable to fully ascertain the roles of Industry, Government and Academia, as the databases did not use standardized terms and definitions, nor did they provide complete data. Moreover, both registries - one of which is proposed to be the mandated repository for clinical trials information - contained data that were inaccurate and erroneous. Conclusions: Collaboration between Industry, Academia and Government is the most frequent mechanism of conducting early phase clinical trials. Although not always the sponsor, Industry plays a dominant role in early phase cancer immunotherapy studies by providing up to 90% of study reagents used. Significant improvements to the current clinical trial registries are required to ensure complete and accurate recording of clinical trial information. Author Disclosure Employment or Leadership Consultant or Advisory Role Stock Ownership Honoraria Research Funding Expert Testimony Other Remuneration LICR
Multi-user architectures for computer-aided engineering collaboration (2011)

Concurrent engineering, defined as product development by teams working in parallel, is severely limited by the serial functionality of modern computer-aided applications (CAx) like CAD/CAE/CAM. Ultimately, any development project is decomposed into assemblies and components which are then sub-divided into single models. Each model is then assigned to one technical person: designer, analyst, or process planner. The function of this paper is to consider CAx architectural changes that will allow product teams to concurrently and simultaneously collaborate on product models. Several prototypes will demonstrate multi-user effectiveness, and expose collaborative and architectural deficiencies in modern CAx tools.
MR guidance, monitoring and control of Brain HIFU therapy in small animals: In vivo demonstration in rats at 7T (2009)

In the framework of HIFU transcranial brain therapy, it is mandatory to develop techniques capable of assessing the focusing quality and location before the treatment. Monitoring heat deposition in real time and verifying the extension of the treated area are also important steps. In this study, an imaging protocol is proposed to:1/ locate the US radiation force induced displacement in tissues and quantify the acoustic pressure at focus prior to HIFU; 2/ monitor the temperature rise during HIFU; and 3/ assess the changes in elasticity in the treated area. A 7T MRI scanner was equipped with a home-made stereotactic frame for rats and a US focused transducer working at 1.5 MHz. Such a tool is key for the evaluation of the biological effects of HIFU on brain tissue and tumors. The proposed protocol was successfully tested on 12 rats with and without injected tumors. The accurate localization of the focal point prior to HIFU was demonstrated in vivo. Furthermore, the pressure estimation in situ allowed to accurately simulate the heat deposition at focus and to plan the treatment (electrical power, duration). The temperature measurements were in good accordance with the predicted curves. The elasticity maps showed significant changes after treatment in some cases.
Digital identity guidelines: federation and assertions (2017)

Certain commercial entities, equipment, or materials may be identified in this document in order to describe an experimental procedure or concept adequately.
THE ATACAMA COSMOLOGY TELESCOPE: EXTRAGALACTIC SOURCES AT 148 GHz IN THE 2008 SURVEY (2011)

Americanae nace como un proyecto conjunto que surge dentro de la Red Europea de Información y Documentación sobre América Latina (REDIAL), y que ha afrontado la Biblioteca de la Agencia Española de Cooperación Internacional para el Desarrollo (AECID). Esta nueva biblioteca virtual hace más accesibles los libros digitales de tema americanista a los investigadores y usuarios interesados de cualquier parte del mundo.
PYROLYSIS UNDER EXTREME HEAT FLUX CHARACTERIZED BY MASS LOSS AND THREE-DIMENSIONAL SCANS (2019)

Abstract not provided.