gebhart/patrepeval huggingface dataset:
Isolation of Murine Hematopoietic Stem Cells and Progenitor Cells (2005)

Protocols for the purification of CD34-KSL cells, CD34+KSL cells, SP cells, and Tip-SP CD34-KSL cells are described. Mouse hematopoietic stem cells are highly enriched in the CD34-KSL and SP fractions. These two populations overlap one another. A nearly homogenous population of HSCs can be obtained by isolating Tip-SP CD34-KSL cells. One of the earliest progenitor cell populations is CD34+KSL cells.
Dual-layer write-once media for 1x-4x-speed recording based on Blu-ray Disc format (2003)

Dual-layer write-once media with Te-O-Pd recording films based on Blu-ray Disc format were shown to have sufficient CNR at recording speed of 1x(5.28m/s) to 4x(21.1m/s), and recording mechanisms were discussed.
Novel self-etch adhesive with antibacterial and protein-repellent functions to prevent enamel demineralization (2018)

Enamel demineralization is the most undesired side-effect of fixed orthodontic treatments. This study were to develop a novel adhesive that is self-etching instead of using the traditional etching method, and to form a sealant on the enamel to prevent demineralization. 2-methacryloyloxyethyl phosphorylcholine (MPC) and quaternary ammonium dimethylaminohexadecyl methacrylate (DMAHDM) were mixed into a self-etch adhesive (Adper Easy One, 3M, St. Paul, MN, USA; referred to as AEO). Enamel shear bond strength (SBS) was measured. Protein adsorption onto the resins was measured. An oral microcosm biofilm model with saliva was tested. Incorporation of 7.5% MPC and 5% DMAHDM into AEO did not reduce the SBS (p>0.1). AEO with 7.5% MPC+5% DMAHDM had protein adsorption that was only 1/18 that of AEO control. AEO with 7.5% MPC+5% DAMHDM had much stronger antibacterial properties (p<0.05). In conclusion, the new self-etch adhesive with MPC and DAMHDM greatly reduced protein adsorption and inhibited biofilm viability.
Exploring the underlying mechanisms of drug‐induced impulse control disorders: a pharmacovigilance‐pharmacodynamic study (2022)

Our results supported the role of D3-receptor agonism in inducing impulsivity in dopamine receptor agonists and identified a potential role of 5-HT1a receptor agonism in antipsychotics. Investigating these receptors may drive towards a better management of drug-induced impulsivity.
Pathway engineering in yeast for synthesizing the complex polyketide bikaverin (2019)

Abstract Fungal polyketides display remarkable structural diversity and bioactivity, and therefore the biosynthesis and engineering of this large class of molecules is therapeutically significant. Here, we successfully recoded, constructed and characterized the biosynthetic pathway of the formation of bikaverin, a tetracyclic polyketide with antibiotic, antifungal and anticancer properties, in S. cerevisiae . We used a green fluorescent protein (GFP) tagging strategy to identify the low expression of Bik1 (polyketide synthase) as the bottleneck step in the pathway, and a promoter exchange strategy to increase expression of Bik1 and bikaverin yield. To further increase product yield, we used an enzyme-fusion strategy to couple the monooxygenase (Bik2) and methyltransferase (Bik3) to efficiently channel intermediates between modifying enzymes, leading to a dramatic improvement of Bikaverin yield of nearly 60-fold. This study demonstrates that the biosynthesis of complex polyketides biosynthesis can be established and efficiently engineered in S. cerevisiae , highlighting the great potential for natural product synthesis and large-scale fermentation in yeast.
Probing crystallographic orientation-specific carrier lifetimes in epitaxial Ge/AlAs and InGaAs/InP heterostructures (2022)

Schematic representation of orientation specific Ge/AlAs/GaAs and InGaAs/InP fin transistor architecture and their carrier lifetimes.
Effect of serum IgG1 to Cryptococcus neoformans glucuronoxylomannan on murine pulmonary infection (1997)

Little is known about the role of Ab in protection against pulmonary fungal pathogens. The ability of murine IgG1 mAb 2H1 to modify pulmonary Cryptococcus neoformans infection was investigated in intratracheal infection. mAb 2H1 binds C. neoformans glucuronoxylomannan. mAb 2H1 was given to A/JCr mice 24 h before infection. Two C. neoformans strains were studied: ATCC 24067 (serotype D) and 62070 (serotype A). Fungal burden was determined by CFU 14 days after infection for both strains, and at 2 h, 24 h, 48 h, 7 days, and 28 days after infection for strain 24067. On day 14, mAb 2H1 treatment reduced CFU in the lung, brain, and liver for strain 62070 infection. Minor reductions in lung CFU followed infection with strain 24067 in mAb 2H1-treated mice, despite prolonged survival. The limited ability of mAb 2H1 to reduce lung CFU may reflect rapid phagocytosis of yeast by alveolar macrophages, seen by electron microscopy 2 h after infection, regardless of whether mice had received mAb. Alveolar macrophages phagocytosed and reduced C. neoformans CFU in vitro only in the presence of mAb 2H1. Differences were apparent in phagocytosis and in vitro killing between strains 24067 and 62070. Serum IgG1 modified the course of pulmonary infection in mice by prolonging survival, reducing CFU, and reducing tissue glucuronoxylomannan Ag. mAb administration was associated with enhanced granulomatous inflammation, but did not prevent infection or dissemination. Despite incomplete protection by serum Ab against pulmonary infection, our results provide encouragement for continued vaccine development.
Real-Time Mass Spectrometric Analysis of Hydrothermal Reaction Products (2017)

This is the first work that describes the application of mass spectrometry to the real-time observation of liquid products of hydrothermal reactions in a continuous reactor. A real-time mass spectrometric analysis (real-time MS analysis) system was developed and designed to elucidate products of glucose decomposition during the hydrothermal treatment process. This was achieved by connecting a continuous-flow reactor with a quadrupole mass analyzer using custom-built connection fittings. The effects of residence time and temperature of hydrothermal treatment on glucose decomposition products were investigated. The temperatures were between 140 and 220 °C, and reaction times were between 5 and 20 min. The real-time MS method investigated the products of glucose decomposition, and cold samples were characterized using high-performance liquid chromatography (HPLC). At 140 °C, glucose decomposition is not complete, which was confirmed by a mass spectrum of a glucose standard. At a temperature of 180 °C or above, fragmentation peaks of fructose at m/z = 131 and 149, ring compound products like 5-hydroxymethylfurfural (5-HMF) at m/z = 97 and 126, and furfural at m/z = 96 and 67 were obtained. Retro-aldol products like glyceraldehyde and glycolaldehyde were also observed, confirmed by m/z = 90, 72, and 61 and an increasing ion current at m/z = 60, 29, and 31, respectively. No fructose was observed using a conventional HPLC at 200 °C for 10 min. The effectiveness of real-time MS analysis is strong, collated with a conventional HPLC method.
Modelling delay in symbolic test for data paths with partial scan (2002)

The problem of handling (pipeline) delay in data paths using a symbolic test methodology is addressed. The authors show that the delay in the incomplete scan path architecture, where only a subset of the registers is included in the scan path in order to eliminate the feedback loops, can be easily modelled and taken into account in the (combinational) symbolic test pattern generator. The pith of the methodology is formed by novel controllability and observability descriptions.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Enhancement of active MMP release and invasive activity of lymph node metastatic tongue cancer cells by elevated signaling via the TNF-α-TNFR1-NF-κB pathway and a possible involvement of angiopoietin-like 4 in lung metastasis (2016)

To study the role of TNF-α in tongue cancer metastasis, we made highly metastatic cells from a human oral squamous cell carcinoma cell line (SAS) by repeating the passage in which the cells were injected into a nude mouse tongue and harvested from metastasized cervical lymph nodes. Cancer cells after 5 passages (GSAS/N5) increased invasive activity 7-fold in a TNF-α receptor 1 (TNFR1)-dependent manner and enhanced mRNA expression of TNF-α and TNFR1. In the highly metastatic cells, NF-κB activation was upregulated via elevated phosphorylation of Akt and Ikkα/β in the signaling pathway and secretion of TNF-α, active MMP-2 and MMP-9 increased. Suppression of increase of TNF-α mRNA expression and MMP secretion by NF-κB inhibitor NBD peptide suggested a positive feedback loop in GSAS/N5 cells; TNF-α activates NF-κB and activated NF-κB induces further TNF-α secretion, leading to increase of active MMP release and promotion of invasion and metastasis of the cells. GSAS/N5 cells that had been injected into the nude mouse tongue and harvested from metastasized lungs multiplied angiopoietin-like 4 (angptl4) expression with enhanced migration activity, which indicated a possible involvement of angptl4 in lung metastasis of the cells. These results suggest that TNF-α and angptl4 promote metastasis of the oral cancer cells, thus, these molecules may be therapeutic targets for patients with tongue cancer.
Role of DRD3 in morphine-induced conditioned place preference using drd3-knockout mice (2004)

The dopamine D3 receptor (DRD3) mediates expression of conditioned effects of psychostimulants, but conflicting results have been obtained with opiates. In a conditioned place preference (CPP) procedure, morphine increased the time spent in a compartment previously paired with drug injection. CPP was obtained at morphine doses of 16 and 32 mg/kg in wild-type (drd3+/+) mice and 8, 16 and 32 mg/kg in DRD3-knockout (drd3-/-) mice. BP897, a DRD3-selective partial agonist, inhibited the expression of morphine-CPP in drd3+/+, but not drd3-/- mice. BP 897 reduced brain regional activation, measured by c-fos imaging after the CPP test session, in the somatosensory cortex of drd3+/+, but not drd3-/- mice. These results confirm the role of DRD3 in the expression of conditioned effects of morphine and the participation of the somatosensory cortex in these effects.
ChemInform Abstract: Synthesis and SAR of Novel, 4‐(Phenylsulfamoyl)phenylacetamide mGlu4 Positive Allosteric Modulators (PAMs) Identified by Functional High‐Throughput Screening (HTS). (2010)

Abstract The title compounds are evaluated as mGlu 4 positive allosteric modulators (PAMs).
2-Aminothienopyridazines as Novel Adenosine A1Receptor Allosteric Modulators and Antagonists (2008)

A pharmacophore-based screen identified 32 compounds including ethyl 5-amino-3-(4- tert-butylphenyl)-4-oxo-3,4-dihydrothieno[3,4- d]pyridazine-1-carboxylate ( 8) as a new allosteric modulator of the adenosine A1 receptor (A1AR). On the basis of this lead, various derivatives were prepared and evaluated for activity at the human A 1AR. A number of the test compounds allosterically stabilized agonist-receptor-G protein ternary complexes in dissociation kinetic assays, but were found to be more potent as antagonists in subsequent functional assays of ERK1/2 phosphorylation. Additional experiments on the most potent antagonist, 13b, investigating A1AR-mediated [(35)S]GTPgammaS binding and [(3)H]CCPA equilibrium binding confirmed its antagonistic mode of action and also identified inverse agonism. This study has thus identified a new class of A1AR antagonists that can also recognize the receptor's allosteric site with lower potency.
High repetition rate compact mode-locked Cr/sup 4+/-doped lasers (1998)

Summary form only given. This is, to our knowledge, the highest output power produced by a femtosecond Cr/sup 4+/:YAG laser to date. Shortening the cavity produced 125-fs duration pulses with 110 mW average output power in a single beam, at a repetition rate of 1 GHz. To produce a more compact and efficient system, we employ a diode-pumped Yb-doped fiber laser as the pump source.
A novel high-volume separation technology base on acoustophoresis for treatment of produced water in the oil and gas industry (2016)

A novel emulsion splitting separation technology has been developed using acoustic radiation forces to recover the dispersed oil phase from an oil water emulsion. Current separation technologies suffer from high energy costs, use of consumables, fouling, and limited efficiency in separation of micron-sized particles. Multi-dimensional ultrasonic standing waves are used to trap a dispersed phase in a fluid. The action of the acoustic forces results in clustering and coalescence of droplets. Upon reaching a critical size, they are continuously separated through enhanced buoyancy. A second mode of operation uses acoustic radiation forces to increase the average droplet size and reduce the sub-20 micron droplet concentration. Earlier work was presented at ICA 2013(Dionne [1]). New results are shown for prototypes with a 1x2, 3x4 and 6x6 inch flow chamber driven by 2 &amp; 3 MHz PZT transducers operating at flowrates of 1L/h, 30L/h, and 227-1136 L/h, tested with produced water samples from four US locations. Measured separation efficiencies of over 90% have been documented as well as particle size shifts of &amp;gt;100 micron. The particle size shift is particularly appealing as it acts as a complimentary technology to existing oil &amp; gas separation technology. [1] J. Dionne, B. McCarthy, B. Ross-Johnsrud, L. Masi, and B. Lipkens, “Large volume flow rate acoustophoretic phase separator for oil water emulsion splitting,” J. Acoust. Soc. Am., Vol. 133, No. 5, Pt. 2, May 2013, pp. 3237. [Work supported by NSF SBIR IIP- 1330287.]
Lymphopenia Prior High-Dose Chemotherapy Is a Major Prognostic Factor for Severe Infectious Complication after Autologous Blood Stem Cell Transplantation for Lymphoma’s Patients. (2006)

Abstract Rationale: Host factors able to predict accurately the infectious risk after high dose chemotherapy (HDCT) and autologous stem cell transplantation (ASCT) would be very useful. We previously reported that lymphopenia immediately prior conventional chemotherapy (day 1) identifies a subgroup of patients at risk of early death (Ray-Coquard et al. Br J Cancer 2001). This model was then investigated for patients receiving HDCT and ASCT. Patients and Methods: Between 1993 and 2000, 146 patients with diffuse large cell lymphoma (69), burkitt lymphoma (2), T lymphoblastic lymphoma (5), mantle cell lymphoma (16), follicular lymphoma (27), MALT lymphoma (2), lymphocytic lymphoma and chronic lymphoid leukemia (7) and hodgkin lymphoma (18) received HDCT with (72) or without (74) Total Body Irradiation (TBI). Median age at time of HDCT was 50 years (range, 38–68). Patients were treated in first (35.6%), second (48.4%) and third or more (16%) line treatment. Complete and unconfirmed complete remission before HDCT was obtained in 45.2%, partial response in 47.9%, minor response in 2.1% and 4.8% of patients were treated in progressive disease. Median transplanted CD34+ cell number was 6.79 × 106/kg (range, 0.70–47.58). 25 patients received G-CSF injection after graft. The predictive value of pre HDCT (d1) lymphocyte count for the risk of infection during the first 100-days after ASCT was investigated on this series. Results: Median d1 lymphocyte count was 750/mm3 (range, 90–3900). Infectious events were 35 clinical severe infectious complications (20 pneumonia, 8 severe enterocolitis, 3 viral infectious, 2 invasive fungal infection, 1 soft tissue infection, 1 severe septicaemia), 8 (6%) intensive care unit transfert and 4 (3%) toxic deaths for infectious reason. Using logistic regression, patients with ≤ 1000 d1 lymphocyte count presented statistically more infectious events (39 vs. 8 p&lt;0.01). 62 patients of this series (69%) had ≤ 1000 d1 lymphocyte count. Age at HDCT, disease type, tumour response before HDCT, used of TBI, performance status before HDCT, number of transplanted CD34+ cells were not predictive of risk of infection. During the hospitalization after graft, used of G-CSF, number of days of broad spectrum antibiotics and anti fungal treatment, documented bacterial infection, number of days of hospitalization, number of transfusion, duration of neutropenia under 500/μl and plaquets recovery were not statistically different between patients with ≤ or &gt; 1000 d1 lymphocyte count. Conclusion: Pre HDCT (d1) lymphocyte count is a potent predictor of the risk of severe infectious complication. These data need to be confirmed in a validation series. As 69% of patients had ≤ 1000 d1 lymphocytes, we incline to analyse lymphocyte subset to provide more discriminative information about the risk of infectious complication after HDCT. This currently being investigated.
Supplementary Figure 4 from SIRT1 Pathway Dysregulation in the Smoke-Exposed Airway Epithelium and Lung Tumor Tissue (2023)

&lt;p&gt;PDF file, 72 K, SIRT1 pathway activation in lung tumor tissue datasets with smoking-status or different tumor subtypes. A. Lung tumor tissue (n=60, 56 adenocarcinomas, 1 squamous, 3 bronchioloalveolar carcinoma) and paired adjacent normal lung tissue (n=60) (GSE19804) from non-smoking women in Taiwan hybridized to Affymetrix HGU133 Plus 2.0 microarrays. There was no different between SIRT1 pathway activation between adjacent normal and tumor tissue. B. Top left. Adjacent normal lung tissue and paired lung tumor tissue (n=42 pairs, n=22 adenocarcinoma, n=9 large cell carcinoma, and n=11 squamous cell carcinoma) (GSE19188) hybridized to Affymetrix HGU133 Plus 2.0 microarrays. SIRT1 pathway activation was decreased between adenocarcinomas and normal lung tissue (ADC, p=0.044) and between large cell carcinomas and normal lung tissue (LCC, p=0.002); however, SIRT1 pathway activation was significantly increased in squamous cell carcinoma compared to adjacent normal lung tissue (SCC, p=0.023). Top middle. Adenocarcinoma (ADC, n=40) and squamous cell carcinoma (SCC, n=18) lung tumor tissue (GSE10245) was hybridized to Affymetrix HGU133 Plus 2.0 microarrays. SIRT1 pathway activation was significantly higher in SCC versus ADC tumors (p&lt;0.001). Top right. Adenocarcinoma (ADC, n=50), large cell carcinoma (LCC, n=20), and squamous cell carcinoma (SCC, n=28) lung tumor tissue (GSE28571) was hybridized to Affymetrix HGU133 Plus 2.0 microarrays. SIRT1 pathway activation was significantly higher in SCC versus ADC tumors (p&lt;0.001). Bottom left. Adenocarcinoma (ADC, n=58) and squamous cell carcinoma (SCC, n=53) lung tumor tissue (GSE3141) was hybridized to Affymetrix HGU133 Plus 2.0 microarrays. SIRT1 pathway activation is significantly lower in ADC versus SCC tumors (p&lt;0.001). Bottom middle. CCLE lung cancer cell lines (n=180) that include n=49 adenocarcinoma (ADC), n=5 bronchioloalveolar adenocarcinoma (BAC), n=14 large cell carcinoma (LCC) , n=4 mixed adenosquamous carcinoma (Mixed ADC/SCC), n=1 mucoepidermoid carcinoma (mucoepidermoid), n=24 non-small cell carcinoma (NSCLC), n=53 small cell carcinoma (SCLC), n=26 squamous cell carcinoma (SCC), n=1 undifferentiated carcinoma (UnDiff), and n=3 Unknown.&lt;/p&gt;
Recrystallisation and texture in hot deformed aluminium alloy 7010 thick plates (1996)

AbstractAbstractThe recrystallisation behaviour and its effect on the crystallographic texture during solution heat treatment of thick aluminium alloy 7010 plates has been studied both on commercially hot rolled plates and on laboratory specimens deformed in plane strain compression. In addition to X-ray macrotexture analysis, individual grain orientation measurements within the recovered subgrains and the recrystallised new grains were performed by means of electron backscattering diffraction. Significant recrystallisation takes place during the solution treatment with almost no change in the macrotexture. This behaviour is explained by the balancing effect of randomisation of the texture during recrystallisation and texture sharpening within the recovered matrix regions.MST/3433
Normal human B cells display ordered light chain gene rearrangements and deletions. (1982)

Human kappa-producing B cell lines and leukemias retain their excluded lambda light chain genes in the germ line configuration, whereas transformed lambda-producing B cells uniformly rearrange or delete their kappa genes (12). Whether the unexpected lambda gene recombinations within malignant lambda-producing B cells reflect a normal developmental process or are secondary to transformation and specific chromosomal translocations was uncertain. To resolve this issue, we purified circulating lambda-bearing B cells from a normal individual to 97% purity by using a series of negative selection steps and a final positive selection on a cell sorter. Over 95% of the collective kappa genes in these lambda B cells were no longer in their germ line form, with the majority (60%) deleted and the remainder present but in a rearranged state. The chromosomal loss of the germ line kappa genes included the joining (J kappa) segments as well as the constant (C kappa) region, yet the particular variable (V kappa) gene family studied was spared. In addition, the incidence of kappa gene deletions was higher in long-term than in freshly transformed lambda B cell lines. This implies that the deletion of aberrantly rearranged kappa genes may occur as a second event. Such a mechanism would serve to eliminate aberrant transcripts and light chain fragments that might interfere with the synthesis and assembly of effective immunoglobulin molecules. Thus, despite the nearly equal usage of kappa and lambda light chain genes in man, there appears to be a sequential order to their expression during normal B cell ontogeny in which kappa gene rearrangements precede those of lambda.
Catheter ablation of left atrial tachycardia adjacent to a septal closure device: A multifaceted challenge? (2023)

Catheter ablation (CA) of left atrial tachycardia adjacent to implanted septal closure devices represents a multifaceted challenge. We describe the case of a 57-year-old patient with remote percutaneous closure of atrial septal defect who underwent successful CA of left atrial tachycardia adjacent to the septal device using a transaortic approach and RF energy. Besides the technical difficulties and associated risks, interference between the device and applied RF parameters may limit ablation efficiency. Further research is required to evaluate the safety, efficacy, and optimal energy type/parameters when ablating arrhythmias adjacent to these devices.
Spatially separated intrinsic emission components inInxGa1−xNternary alloys (2009)

The characteristics of band-edge photoluminescence (PL) from Ga-rich ${\text{In}}_{x}{\text{Ga}}_{1\ensuremath{-}x}\text{N}$ ternary-alloy epitaxial layers with indium compositions of $x=0.02$, 0.03, 0.05, 0.06, and 0.09 have been comprehensively studied by means of scanning near-field optical microscopy (SNOM) in addition to conventional macroscopic PL spectroscopy. The band-edge PL from the ternary alloys consisted of two intrinsic emission components, a strong higher-energy component and a weak lower-energy component. The radiative recombination channels of the two components were not independent of each other and thermal population of carriers took place between the components. A spatial separation between the two components was clearly indicated by SNOM-PL measurements. The higher-energy component corresponded to islandlike regions with diameters of several hundred nanometers whereas the lower-energy component corresponded to dotlike regions with diameters of 50--80 nm. In addition, the regions corresponding to the lower-energy component were surrounded by a local potential maximum, which acted as a potential barrier for carriers between the higher- and the lower-energy components.