gebhart/patrepeval huggingface dataset:
Marked telomere fluctuation of leukocytes during graft-versus-host disease in allogeneic stem cell transplantation (2005)

Immune dysfunction after allogeneic stem cell transplantation (SCT) is closely associated with cell turnover of lymphocytes and homeostasis of hematopoietic stem cells. Telomeres, repetitive sequences (TTAGGG)n on the end of linear chromosomes, reflect the mitotic history of stem cells. Using telomere fluorescence in situ hybridization (FISH) and flow cytometry (flow-FISH), we measured telomere length in lymphocytes and neutrophils at various intervals to analyze the relationship between telomere length change and clinical features in 5 patients who underwent allogeneic bone marrow transplantation. During the first year after allogeneic stem cell transplantation, a marked fluctuation of telomere length in peripheral blood leukocytes was observed in all recipients, and in 3 patients there was a reduction of telomere length during chronic graft-versus-host disease (GvHD) or during post-transplant lymphoproliferative disorder. The reduction of telomere length during GvHD was evident in lymphocytes and neutrophils, but telomere length in neutrophils tended to recover earlier than that observed in lymphocytes. The rapid reduction of telomere length in leukocytes during GvHD was too extensive to be explained by the end-replication problem, suggesting the presence of a telomerically unstable hematopoietic condition after transplant in vivo.
Genetic Vaccines and Therapy (2015)

The clinical application of monoclonal antibodies (mAbs) potentially concerns a wide range of diseases including, among others, viral infections, cancer and autoimmune diseases. Although intravenous infusion appears to be the simplest and most obvious mode of administration, it is very often not applicable to long-term treatments because of the restrictive cost of mAbs certified for human use and the side effects associated with injection of massive doses of antibodies. Gene/cell therapies designed for sustained and, possibly, regulatable in vivo production and systemic delivery of mAbs might permit to advantageously replace it. We have already shown that several such approaches allow monthto year-long ectopic antibody production by non-B cells in living organisms. Those include grafting of ex vivo genetically modified cells of various types, in vivo adenoviral gene transfer and implantation of encapsulated antibody-producing cells. Because intramuscular electrotransfer of naked DNA has already been used for in vivo production of a variety of proteins, we have wanted to test whether it could be adapted to that of ectopic mAbs as well. We report here that this is actually the case since both long-term and regulatable production of an ectopic mAb could be obtained in the mouse taken as a model animal. Although serum antibody concentrations obtained were relatively low, these data are encouraging in the perspective of future therapeutical applications of this technology in mAb-based immunotherapies, especially in developing countries where cost-effective and easily implementable technologies would be required for large-scale applications in the context of severe chronic viral diseases such as HIV and HCV infections. Findings The therapeutical potential of monoclonal antibodies (mAbs) is enormous. Twelve mAbs have already been approved by the US Food and Drug Administration for therapeutical use and 400 others are currently under clinical evaluation [1,2]. However, a number of hurdles have still to be overcome before efficient therapeutical application of mAbs on large scales and at reasonable costs. This Published: 23 March 2004 Genetic Vaccines and Therapy 2004, 2:2 Received: 23 December 2003 Accepted: 23 March 2004 This article is available from: http://www.gvt-journal.com/content/2/1/2 © 2004 Perez et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
Methodology to optimize the cost/performance of disk subsystems (2002)

The storage hierarchy plays a major role in the price and performance of high-end enterprise servers. In fact, the total price of a high-end server's hardware is dominated by its memory and disk configuration. Similarly, the performance is significantly influenced by the design and configuration of the server's memory and disk subsystems. Given these realities, the design and development of future server systems requires special attention to the storage subsystem. The paper describes a methodology that can be applied to optimize the disk size and RAID type used in key high-end server workloads.
Isolation of 1,4-Li2-C6H4 and its reaction with [(Ph3P)AuCl] (2010)

The difficulty in generating 1,4-Li2-C6H4 utilising the lithium halogen exchange reaction on 1,4-Br2-C6H4, 1,4-I2-C6H4 and 1-Br-4-I-C6H4 is revisited and only on treatment of 1,4-I2-C6H4 with 2 molar equivalents of n-BuLi can 1,4-Li2-C6H4 1 be isolated in excellent yield. Treatment of 1 with two equivalents of [ClAu(PPh3)] gives [1,4-(Ph3PAu)2-C6H4] 2a in excellent yield. Subsequent treatment of 2a with 2.5 molar equivalents of PPh2Me, PPhMe2 or PMe3 affords the PPh3 substituted compounds [1,4-(LAu)2-C6H4] (L = PPh2Me 2b, PPhMe2 2c, PMe3 2d) in essentially quantitative yields. On treatment of 1,4-Br2-C6H4 or 1-Br-4-I-C6H4 with 2 molar equivalents of n-BuLi only mono-lithiation takes place to give 1-Br-4-Li-C6H4 3 as shown through the isolation of essentially 1:1 molar equivalents of Ph2PC6H4-4-Br and Ph2PBu on treatment with 2 molar equivalents of ClPPh2. Treatment of 3, prepared by lithium/iodine exchange on 1-Br-4-I-C6H4, with [ClAu(PPh3)] affords [(Ph3P)Au(C6H4-4-Br)] 4 as expected and in addition [(Ph3P)Au(n-Bu)(C6H4-4-Br)2] 5, indicating the straightforward chloride/aryl exchange at gold may proceed in competition with oxidative addition of the n-BuI, generated in the initial lithium/iodine exchange reaction, to some aurate complex Li[Au(C6H4-4-Br)2] 6 formed in situ followed by reductive elimination of Br-C6H4-4-n-Bu in a manner that mimics lithium diorganocuprate chemistry. All of the gold-containing compounds have been spectroscopically characterised by 1H and 31P-{1H} NMR and in addition compounds 2a-d and 5 by single crystal X-ray diffraction studies. The solid state structures observed for 2a-d are dictated by non-conventional hydrogen bonding and the packing requirements of the phosphine ligands. For 2a and 2b there is no close Au...Au approach, however for 2c and 2d the reduction in the number of phenyl rings allows the formation of Au...Au contacts. For 2c and 2d the extended structures appear to be helical chains with Au...Au contact parameters of 3.855(5) A and C-Au-Au-C 104.1(3)degrees for 2c and 3.139(4) A and C-Au-Au-C -92.0(2)degrees for 2d. The Au...Au approach in 2c is longer than is normally accepted for an AuAu contact and is dictated by ligand directed non-conventional hydrogen bonding to the aurated benzene ring and the pi-stacking requirements of the phosphine ligand. By comparison of the structures 2a-2d with other structures in the database it is evident that the aurophilic interaction is a poor supramolecular synthon in the presence of non-conventional hydrogen bond donors. Searches of the CCDC database suggest that the observed parameters for the Au...Au contact in 2c sit close to the cut-off point for observing this type of contact. In addition to aurophilic contacts and non-conventional hydrogen bonds there are a number of halogenated solvent C-Cl...Au contacts observed in the structures of 2a and 2d. The nature of these contacts have implications for the accepted van der Waals radius of gold which should be extended to 2 A.
Tunable quantum-cascade laser pumped molecular lasers for terahertz imaging (2020)

We demonstrate a new class of optically pumped far-infrared lasers using quantum cascade lasers as the pump. Following an initial demonstration using nitrous oxide, we show how we can generate light at THz frequencies using different gases.
Supplementary Figure 4 from p38γ Promotes Breast Cancer Cell Motility and Metastasis through Regulation of RhoC GTPase, Cytoskeletal Architecture, and a Novel Leading Edge Behavior (2023)

&lt;p&gt;PDF file - 148K&lt;/p&gt;
Evolutionary optimization of the catalytic effectiveness of an enzyme (1989)

The kinetic and thermodynamic features of reactions catalyzed by present-day enzymes appear to be the consequence of the evolution of these proteins toward maximal catalytic effectiveness. These features are identified and analyzed (in detail for one substrate-one product enzymes) by using ideas that link the energetics of the reaction catalyzed by an enzyme to the maximization of its catalytic efficiency. A catalytically optimized enzyme will have a value for the "internal" equilibrium constant (Kint, the equilibrium constant between the substrates and the products of the enzyme when all are bound productively) that depends on how close to equilibrium the enzyme maintains its reaction in vivo. Two classes are apparent. For an enzyme that operates near equilibrium, the catalytic efficiency is sensitive to the value of Kint, and the optimum value of Kint is near unity. For an enzyme that operates far from equilibrium, the catalytic efficiency is less sensitive to the value of Kint, and Kint assumes a value that ensures that the rate of the chemical transformation is equal to the rate of product release. In each of these cases, the internal thermodynamics is "dynamically matched", where the concentrations of substrate- and product-containing complexes are equal at the steady state in vivo.
Effect of Grit-Blasting on the Surface Energy of Graphite/Epoxy Composites (2005)

ABSTRACT Contact-angle measurements were used to determine the surface energies of graphite/epoxy composites before and after grit-blasting with 80- and 220-grit garnet particles. Two different composite systems cured at 350°F were considered but they behaved similarly. Contact angles made by a series of liquids, including water, ethylene glycol, glycerol, formamide, and methylene iodide on as-tooled and grit-blasted composite panels were measured using a contact-angle goniometer. The contact angles were used to determine the dispersive and polar components of the surface energy . However, instead of using the contact angles made by two liquids to determine the two components of the surface energy, we used the contact angles made by several liquids and a graphical technique to determine and to improve the accuracy of the measurements. It was found that the surface energies of as-tooled composites were approximately 35 mJ/m2 and were mostly dispersive; the polar components were small. After grit-blasting with 80- or 220-grit garnet, the dispersive component of the surface energy decreased somewhat whereas the polar component increased significantly; the total surface energy after grit-blasting with 80- or 220-grit garnet was approximately 50 mJ/m2. Etching composites in oxygen plasmas had a similar effect on the dispersive and polar components of the surface energy and on the total surface energy. Grit-blasting with 220-grit alumina resulted in higher dispersive components of surface energy and lower polar components than grit-blasting with 220-grit garnet even though the total surface energy was similar after grit-blasting with 220-grit garnet and alumina. Correcting the measured surface energies for the effects of roughness resulted in small decreases in the total surface energy of the grit-blasted composites. However, even after correcting for roughness, the surface energies of the grit-blasted composites were still significantly greater than those of the as-tooled composites, indicating that grit-blasting resulted in changes in surface chemistry as well as changes in surface morphology of the composites. These changes in surface chemistry certainly included the removal of mold release agents from the as-tooled composites but probably also involved the creation of free radicals and their reaction with atmospheric oxygen to introduce oxygen-containing functional groups on the surfaces of the composites.
<title>Mechanics of polarization ray tracing</title> (1992)

Polarization ray tracing, which consist of several extensions to geometrical ray tracing, calculates the evolution of polarization states along ray paths and determines the intrinsic polarization properties, such as diattenuation and retardance, associated with ray paths. This paper compares the suitability of the Jones, Mueller, and a three-dimensional polarization ray tracing calculi, examining the issues of local versus global coordinates, amplitude versus phase representations, inclusion of the wavefront aberration function, partially polarized light, measurements of images by polarimeters, and diffraction image formation by low and high numerical aperture beams.
Principles and Tools for Collaborative Entity-Based Intelligence Analysis (2009)

Software tools that make it easier for analysts to collaborate as a natural part of their work will lead to better analysis that is informed by more perspectives. We are interested to know if software tools can be designed that support collaboration even as they allow analysts to find documents and organize information (including evidence, schemas, and hypotheses). We have modified the Entity Workspace system, described previously, to test such designs. We have evaluated the resulting design in both a laboratory study and a study where it is situated with an analysis team. In both cases, effects on collaboration appear to be positive. Key aspects of the design include an evidence notebook optimized for organizing entities (rather than text characters), information structures that can be collapsed and expanded, visualization of evidence that emphasizes events and documents (rather than emphasizing the entity graph), and a notification system that finds entities of mutual interest to multiple analysts. Long-term tests suggest that this approach can support both top-down and bottom-up styles of analysis.
Leucine-Rich Repeat Neuronal Protein 1 Regulates Differentiation of Embryonic Stem Cells by Post-Translational Modifications of Pluripotency Factors (2018)

Stem cell surface markers may facilitate a better understanding of stem cell biology through molecular function studies or serve as tools to monitor the differentiation status and behavior of stem cells in culture or tissue. Thus, it is important to identify additional novel stem cell markers. We used glycoproteomics to discover surface glycoproteins on human embryonic stem cells (hESCs) that may be useful stem cell markers. We found that a surface glycoprotein, leucine-rich repeat neuronal protein 1 (LRRN1), is expressed abundantly on the surface of hESCs before differentiation into embryoid bodies (EBs). Silencing of LRRN1 with short hairpin RNA (shLRRN1) in hESCs resulted in decreased capacity of self-renewal, and skewed differentiation toward endoderm/mesoderm lineages in vitro and in vivo. Meanwhile, the protein expression levels of the pluripotency factors OCT4, NANOG, and SOX2 were reduced. Interestingly, the mRNA levels of these pluripotency factors were not affected in LRRN1 silenced cells, but protein half-lives were substantially shortened. Furthermore, we found LRRN1 silencing led to nuclear export and proteasomal degradation of all three pluripotency factors. In addition, the effects on nuclear export were mediated by AKT phosphorylation. These results suggest that LRRN1 plays an important role in maintaining the protein stability of pluripotency factors through AKT phosphorylation, thus maintaining hESC self-renewal capacity and pluripotency. Overall, we found that LRRN1 contributes to pluripotency of hESC by preventing translocation of OCT4, NANOG, and SOX2 from nucleus to cytoplasm, thereby lessening their post-translational modification and degradation. Stem Cells 2018;36:1514-1524.
Metal-Free and User-Friendly Regioselective Hydroxyfluorination of Olefins (2018)

A simple, user-friendly, metal-free protocol for the regioselective anti-Markovnikov hydrofluorination of olefins using readily available and inexpensive reagents has been developed. This new approach displays a broader scope than previously reported methodologies and has been applied to the late-stage fluorination of a complex molecule, giving rise to a fluorosteroid derivative. The stereochemistry of the process has also been studied in some detail.
ISIS Extensions for BIER in Non-MPLS Networks (2018)

Bit Index Explicit Replication (BIER) [RFC8279] is an architecture
that provides multicast forwarding through a domain without
requiring intermediate routers to maintain multicast related per-flow
state. BIER can be supported in MPLS and non-MPLS networks. The common
BIER header format and encapsulation for MPLS and non-MPLS networks is
specified in [RFC8296]. BIER in Ethernet encapsulation is an example
of BIER encapsulation in non-MPLS networks. [RFC8401] specifies the
required extensions to the IS-IS [RFC1195] protocol for the
distribution of BIER sub-domain information including the Sub-sub-TLV
required to support BIER in MPLS encapsulation for MPLS networks.
This document specifies the required extensions to the IS-IS [RFC1195]
protocol for supporting BIER in non-MPLS networks using BIER in
Ethernet encapsulation.
Characterization of Recombinant B. abortus Strain RB51SOD Toward Understanding the Uncorrelated Innate and Adaptive Immune Responses Induced byRB51SOD Compared to Its Parent Vaccine Strain RB51 (2011)

Brucella abortus is a Gram-negative, facultative intracellular pathogen for several mammals, including humans. Live attenuated B. abortus strain RB51 is currently the official vaccine used against bovine brucellosis in the United States and several other countries. Overexpression of protective B. abortus antigen Cu/Zn superoxide dismutase (SOD) in a recombinant strain of RB51 (strain RB51SOD) significantly increases its vaccine efficacy against virulent B. abortus challenge in a mouse model. An attempt has been made to better understand the mechanism of the enhanced protective immunity of RB51SOD compared to its parent strain RB51. We previously reported that RB51SOD stimulated enhanced Th1 immune response. In this study, we further found that T effector cells derived from RB51SOD-immunized mice exhibited significantly higher cytotoxic T lymphocyte activity than T effector cells derived from RB51-immunized mice against virulent B. abortus-infected target cells. Meanwhile, the macrophage responses to these two strains were also studied. Compared to RB51, RB51SOD cells had a lower survival rate in macrophages and induced lower levels of macrophage apoptosis and necrosis. The decreased survival of RB51SOD cells correlates with the higher sensitivity of RB51SOD, compared to RB51, to the bactericidal action of either Polymyxin B or sodium dodecyl sulfate (SDS). Furthermore, a physical damage to the outer membrane of RB51SOD was observed by electron microscopy. Possibly due to the physical damage, overexpressed Cu/Zn SOD in RB51SOD was found to be released into the bacterial cell culture medium. Therefore, the stronger adaptive immunity induced by RB51SOD did not correlate with the low level of innate immunity induced by RB51SOD compared to RB51. This unique and apparently contradictory profile is likely associated with the differences in outer membrane integrity and Cu/Zn SOD release.
Correction for Stewart-Jones et al., Rational development of high-affinity T-cell receptor-like antibodies (2009)

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans the biological, physical, and social sciences.
Dynamic Behavior of Current Controllers for Selective Harmonic Compensation in Three-Phase Active Power Filters (2013)

Current regulators are a critical part of active power filters (APFs). The design of current regulators capable of compensating high-frequency harmonics created by nonlinear loads is a challenging task. Selective harmonic current compensation using harmonic regulators is a viable method to achieve this goal. However, their design and tuning is not an easy task. The performance-and even the stability-of harmonic current regulators strongly depends on implementation issues, with the tuning of the controller gains being critical. Furthermore, the presence of multiple current regulators working in parallel can create unwanted couplings with the fundamental current regulator, which can result in a deterioration of APF current control, i.e., oscillations and settling times larger than expected. This paper addresses the design and tuning of selective harmonic compensators, with a focus on their stability analysis and transient behavior.
Controlled Self‐Assembly of Re‐engineered Insulin by FeII (2011)

Self-assembly of proteins mediated by metal ions is crucial in biological systems and a better understanding and novel strategies for its control are important. An abiotic metal ion ligand in a protein offers the prospect of control of the oligomeric state, if a selectivity over binding to the native side chains can be achieved. Insulin binds Zn(II) to form a hexamer, which is important for its storage in vivo and in drug formulations. We have re-engineered an insulin variant to control its self-assembly by covalent attachment of 2,2'-bipyridine. The use of Fe(II) provided chemoselective binding over the native site, forming a homotrimer in a reversible manner, which was easily followed by the characteristic color of the Fe(II) complex. This provided the first well-defined insulin trimer and the first insulin variant for which self-assembly can be followed visually.