gebhart/patrepeval huggingface dataset:
Protein phosphatase 1 modulates the inhibitory effect of With-no-Lysine kinase 4 on ROMK channels (2012)

With-no-Lysine kinase 4 (WNK4) inhibited ROMK (Kir1.1) channels and the inhibitory effect of WNK4 was abolished by serum-glucocorticoid-induced kinase 1 (SGK1) but restored by c-Src. The aim of the present study is to explore the mechanism by which Src-family tyrosine kinase (SFK) modulates the effect of SGK1 on WNK4 and to test the role of SFK-WNK4-SGK1 interaction in regulating ROMK channels in the kidney. Immunoprecipitation demonstrated that protein phosphatase 1 (PP1) binds to WNK4 at amino acid (aa) residues 695-699 (PP1(#1)) and at aa 1211-1215 (PP1(#2)). WNK4(-PP1#1) and WNK4(-PP1#2), in which the PP1(#1) or PP1(#2) binding site was deleted or mutated, inhibited ROMK channels as potently as WNK4. However, c-Src restored the inhibitory effect of WNK4 but not WNK4(-PP1#1) on ROMK channels in the presence of SGK1. Moreover, expression of c-Src inhibited SGK1-induced phosphorylation of WNK4 but not WNK4(-PP1#1) at serine residue 1196 (Ser(1196)). In contrast, coexpression of c-Src restored the inhibitory effect of WNK4(-PP1#2) on ROMK in the presence of SGK1 and diminished SGK1-induced WNK4 phosphorylation at Ser(1196) in cells transfected with WNK4(-PP1#2). This suggests the possibility that c-Src regulates the interaction between WNK4 and SGK1 through activating PP1 binding to aa 695-9 thereby decreasing WNK4 phosphorylation and restoring the inhibitory effect of WNK4. This mechanism plays a role in suppressing ROMK channel activity during the volume depletion because inhibition of SFK or serine/threonine phosphatases increases ROMK channel activity in the cortical collecting duct of rats on a low-Na diet. We conclude that regulation of phosphatase activity by SFK plays a role in determining the effect of aldosterone on ROMK channels and on renal K secretion.
Surface Water Mapping in Sentinel-1 Images: A Probabilistic Approach Combining Classic Detection Methods (2022)

Surface water mapping in satellite images enables flood monitoring, a task with increasing importance under changing climate conditions. Current segmentation methods based on Deep Learning require large, curated datasets for training’ which are difficult to obtain. In this paper, we present a probabilistic approach to water segmentation based on established computer vision methods that requires little training and is easy to interpret. We use prior knowledge of typical backscatter intensity to locate seed pixels likely to be in water and land. A preliminary rough segmentation using thresholding guides the selection of two image patches that will be fully labeled using seeded region growing segmentation. Then, we sample small patches within the automatically labeled regions to train a fully connected neural network that, running in sliding windows, scores for the presence of water in the entire image. The approach is tested for mapping surface water during a large flood event in Aude, France. Outputs of the proposed approach are compared to the reference flood delineation map provided online by the Copernicus service. A F1 score of 0.67 suggests that performance of the proposed approach is similar or better than classic thresholding methods used as benchmark.
Sequence Multiplicity within Spherical Nucleic Acids (2020)

The synthesis and evaluation of spherical nucleic acids (SNAs) incorporating two physically and chemically distinct classes of oligonucleotides (ODNs) at programmed ratios are described. These SNAs are single entity agents that enter the same target cell at defined stoichiometries, and as such allow one to control important cell signaling and regulatory processes. To study the effect of sequence multiplicity within such structures, we synthesized SNAs consisting of a mixture of class A CpG and class B CpG, immunostimulatory ODNs that activate two different toll-like receptor 9 signaling pathways, each in a sequence-specific fashion. These dual-CpG SNAs exhibit high cellular uptake and codelivery of the two ODNs, relative to mixtures of the linear ODN counterparts, and remain highly associated inside the cell over time. Furthermore, the dual-CpG SNAs augment dendritic cell maturation, compared to the same amounts of oligonucleotides delivered in linear or SNA form but not conjugated to one another. Consequently, these structures constitute a platform for designing oligonucleotide-based combination therapeutics with highly tailorable activities.
XMetS potentiates insulin-dependent Akt and Erk phosphorylation. (2015)

&lt;p&gt;&lt;b&gt;A.&lt;/b&gt; CHO-hINSR cells were preincubated for 10 minutes at 37°C with either the IgG2 form of XMetS, the Fab form of XMetS, or control antibody followed by a 10 minute incubation with increasing concentrations of insulin. Phosphorylation of Akt was then determined. &lt;b&gt;B&lt;/b&gt;. CHO-hINSR cells were preincubated for 10 minutes at 37°C with a wide range of XMetS concentrations followed by a 10 minute incubation with increasing concentrations of insulin. Schild analysis &lt;a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088684#pone.0088684-Schild1" target="_blank"&gt;[30]&lt;/a&gt; of these data was carried out using a curve fit algorithm (Prism, allosteric EC&lt;sub&gt;50&lt;/sub&gt; shift). &lt;b&gt;C.&lt;/b&gt; EC&lt;sub&gt;50&lt;/sub&gt; values were determined from each curve fit (of the data presented in B) and are plotted as a function of XMetS concentration. &lt;b&gt;D&lt;/b&gt;. CHO-hINSR cells were preincubated for 10 minutes at 37°C with either XMetS or control antibody followed by a 10 minute incubation with increasing concentrations of insulin and phosphorylation of Erk was then determined. The mean of duplicate determinations are shown.&lt;/p&gt;
Poly(lactide)-Poly(ethylene oxide)-Poly(lactide) Triblock Copolymers: Synthesis and Thermal Properties (2006)

Poly (lactide) - poly (ethylene oxide) - poly (lactide) [PLAPEO-PLA] triblock copolymers are of particular interest in the biomedical field due to their biocompatibility and their ability to form hydrogels in water. These hydrogels have potential applications in both drug delivery applications and tissue engineering. They are particularly interesting because their mechanical properties can be influenced by both the length of the PLA hydrophobic endblocks and by the ability of the PLA end-blocks to crystallize. Copolymers with crystallizable PLLA end-groups form stiffer gels, while copolymers with amorphous stereorandom PLA form weaker hydrogels. We investigate here the thermal properties of these triblock copolymers in their neat state to establish the presence of crystalline PLLA before incorporation into hydrogels and the effect of aging.
Telerobotic Remote Handling of Protein Crystals via an Internet Link (2001)

A combined University / Industry team has developed a prototype system for handling protein crystals aboard the space station. The system used a miniature direct drive robot, CCD television cameras, and a client-server computing system using internet protocols to support the capture of protein crystals from aqueous growth solutions. The system was demonstrated between Huntsville AL. and Seattle WA. An operator in Huntsville controlled the mini robot by invoking pre-defined relative and absolute macros. The movement macros (a predefined sequence of multi-device movement commands) were developed to support precision motion between task locations in the glovebox. The oper-ator invoked the macros by clicking icons in the remote control interface. The system is a promising start for the development of a space-station based remote protein crystal analysis facility.
The Omnitrap Platform: A Versatile Segmented Linear Ion Trap for Multidimensional Multiple-Stage Tandem Mass Spectrometry (2022)

Multidimensional multiple-stage tandem processing of ions is demonstrated successfully in a novel segmented linear ion trap. The enhanced performance is enabled by incorporating the entire range of ion activation methods into a single platform in a highly dynamic fashion. The ion activation network comprises external injection of reagent ions, radical neutral species, photons, electrons, and collisions with neutrals. Axial segmentation of the two-dimensional trapping field provides access to a unique functionality landscape through a system of purpose-designed regions for processing ions with maximum flexibility. Design aspects of the segmented linear ion trap, termed the Omnitrap platform, are highlighted, and motion of ions trapped by rectangular waveforms is investigated experimentally by mapping the stability diagram, tracing secular frequencies, and exploring different isolation techniques. All fragmentation methods incorporated in the Omnitrap platform involving radical chemistry are shown to provide complete sequence coverage for partially unfolded ubiquitin. Three-stage (MS3) tandem mass spectrometry experiments combining collision-induced dissociation of radical ions produced by electron meta-ionization and further involving two intermediate steps of ion isolation and accumulation are performed with high efficiency, producing information rich spectra with signal-to-noise levels comparable to those obtained in a two-stage (MS2) experiment. The advanced capabilities of the Omnitrap platform to provide in-depth top-down MSn characterization of proteins are portrayed. Performance is further enhanced by connecting the Omnitrap platform to an Orbitrap mass analyzer, while successful integration with time-of-flight analyzers has already been demonstrated.
Privacy Preserving Queries on Directed Graph (2018)

In this work, a trust relation determines whether a certain user (at source-host) has permission to access another user (at target-host) by utilising an authentication key with unique fingerprint F. These trust relations can be interpreted as a directed graph, where nodes are user-host pairs and edges are fingerprints. We study the problem of constructing a privacy preserving trust relation data structure. Moreover, we design and prototype a querying mechanism for this data structure and show that it is feasible in practice. We validate our design by utilising real world data.
Advanced carbon supported PtRu catalysts for DMFC (2005)

The PEMFCs are the most suitable FCs for automotive application and those with methanol fuel (DMFC) compared to those with H2 present the advantage of an easier fuel feeding and storage and the disadvantage to require an efficient electrocatalyst also at the negative electrode for methanol oxidation. The carbon supported PtRu catalysts are recognized to be the most promising, and an optimized Pt to Ru atomic ratio, nanometric dimensions of the particles and their uniform distribution on carbon support improve the catalytic activity towards the methanol oxidation.&#13;\nTo enhance the mass- specific activity of carbon supported PtRu catalysts we focused our efforts on PtRu catalysts electrodeposited on carbon-Nafion supports prepared by spray technique with carbons of different specific surface area from 254 m2g-1 to 1040 m2g-1. &#13;\nThe results of the electrochemical, structural and morphological characterisations of such supported PtRu catalysts are presented in this contribution to evidence how carbon-Nafion supports of different accessible surface area, different conditions and electrodeposition techniques, and different Pt to C mass ratio, affect the mass- specific activity of the PtRu catalysts for methanol oxidation in DMFC.
Single-Atom Iridium-Catalyst-Embedded Zeolitic Imidazolate Frameworks for CO2 and Glycerol Transformations (2022)

Developing efficient immobilization techniques for organometallic complexes is essential for the fabrication of efficient single-atom catalysts (SACs) and the recycling of high-performance molecular catalysts. Here, we demonstrate facile methods for preparing SACs by incorporating an Ir complex into a zeolitic imidazolate framework (ZIF) support. The Ir(bis-carbene) complex was introduced at different stages of the ZIF-7-III synthesis, resulting in different immobilization efficiencies, particle shapes, and catalytic activities. The possible local structures were proposed by density functional theory modeling and compared with the X-ray absorption spectroscopy results, which established that the Ir(bis-carbene) moiety coordinates with the defective −N site of the benzimidazolate ligand. The prepared catalysts exhibited unprecedented turnover numbers (>30,000) for glycerol dehydrogenation and CO2 hydrogenation. Hot filtration and recycling tests confirmed the heterogeneity and stability of the catalyst under harsh reaction conditions. The findings of this study will promote the heterogenization of various organometallic complexes for diverse catalytic reactions.
Vasoactive Intestinal Peptide Receptors in Human Platelet Membrane. Characterization of Binding and Functional Activity (1988)

Peer Reviewed
SHP-2 activates signaling of the nuclear factor of activated T cells to promote skeletal muscle growth (2006)

The formation of multinucleated myofibers is essential for the growth of skeletal muscle. The nuclear factor of activated T cells (NFAT) promotes skeletal muscle growth. How NFAT responds to changes in extracellular cues to regulate skeletal muscle growth remains to be fully defined. In this study, we demonstrate that mice containing a skeletal muscle-specific deletion of the tyrosine phosphatase SHP-2 (muscle creatine kinase [MCK]-SHP-2 null) exhibited a reduction in both myofiber size and type I slow myofiber number. We found that interleukin-4, an NFAT-regulated cytokine known to stimulate myofiber growth, was reduced in its expression in skeletal muscles of MCK-SHP-2-null mice. When SHP-2 was deleted during the differentiation of primary myoblasts, NFAT transcriptional activity and myotube multinucleation were impaired. Finally, SHP-2 coupled myotube multinucleation to an integrin-dependent pathway and activated NFAT by stimulating c-Src. Thus, SHP-2 transduces extracellular matrix stimuli to intracellular signaling pathways to promote skeletal muscle growth.
Performance and plasma amino acids of growing calves fed corn silage supplemented with ground soybeans, fishmeal and rumen-protected lysine. (1990)

A 100-d growth study was conducted to evaluate performance and plasma amino acid (AA) responses of 96 crossbred beef calves (220 kg) with ad libitum access to corn silage and supplemented with ground soybeans (GSB) with or without added fishmeal (FM) and (or) rumen-protected lysine (Lys). Calves were allotted by breed, sex and weight to four treatments with three replicate pens of eight calves per pen. The treatments were: GSB, GSB + Lys, GSB + FM and GSB + FM + Lys. The isonitrogenous supplements were top-dressed on corn silage once daily at a level of 2.27 kg/hd, with FM providing one-half of the supplemental N in FM-containing supplements. The Lys-containing supplements provided a daily intake of 6.0 g/hd of rumen-protected Lys. Dry matter intake was similar (P less than .10) for all treatments. Overall ADG and feed efficiency of GSB calves averaged .83 kg/d and 7.39 kg feed/kg gain, respectively, and were 14% lower than the mean of calves fed supplements containing FM and(or) Lys. Lysine was not the principal factor limiting growth because the inclusion of Lys alone in the GSB-containing supplements did not improve (P greater than .10) ADG, feed efficiency or plasma AA concentrations. In contrast, FM supplementation increased (P less than .05) ADG, feed efficiency and plasma concentrations of total AA, total essential AA and total nonessential AA. The inclusion of Lys in the GSB + FM-containing supplement resulted in no further improvement (P greater than .10) in performance.(ABSTRACT TRUNCATED AT 250 WORDS)
Synthesis and olefin polymerization performance of new ansa-zirconocene with OSiO-bridged bis(2-indenyl) ligand (2020)

New ansa-zirconocene with bis(inden-2-yloxy)dimethylsilane ligand was synthesized and characterized by NMR spectroscopy and X-ray crystallography. The zirconocene was found to be highly active catalyst of ethylene polymerization and ethylene/hex-1-ene copolymerization upon methylaluminoxane activation.
European Heart Rhythm Association (EHRA)/Heart Rhythm Society (HRS)/Asia Pacific Heart Rhythm Society (APHRS)/Latin American Heart Rhythm Society (LAHRS) expert consensus on risk assessment in cardiac arrhythmias: use the right tool for the right outcome, in the right population (2020)

In clinical practice and for scientific purposes, cardiologists and primary care physicians perform risk assessment in patients with cardiac diseases or conditions with high risk of developing such. The European Heart Rhythm Association (EHRA), Heart Rhythm Society (HRS), Asia Pacific Heart Rhythm Society (APHRS), and the Latin American Heart Rhythm Society (LAHRS) set down this expert consensus statement task force to summarize the consensus regarding risk assessment in cardiac arrhythmias. Objectives were to raise awareness of using the right risk assessment tool for a given outcome in a given population, and to provide physicians with practical proposals that may lead to rational and evidence-based risk assessment and improvement of patient care in this regard. A large variety of methods are used for risk assessment and choosing the best methods and tools hereof in a given situation is not simple. Even though parameters and test results found associated with increased risk of one outcome (e.g. death) may also be associated with higher risk of other adverse outcomes, specific risk assessment strategies should be used only for the purposes for which they are validated. The work of this task force is summarized in a row of consensus statement tables.
High-performance HIT solar cells for thinner silicon wafers (2010)

We have been researching and developing HIT (Heterojunction with Intrinsic Thin-layer) solar cells to obtain high conversion efficiency. Last year, we updated the world's highest conversion efficiency, which was previously 22.3%, to 23.0% with a practical-sized HIT solar cell at the R&D stage. We have also been investigating the performance of thinner HIT solar cells using less than 100-μm-thick crystalline Si (c-Si) wafers in order to effectively reduce the production cost. By using improved technologies, we succeeded in gaining the high conversion efficiency of 22.8% in a HIT solar cell with a 98-μm-thick c-Si wafer and an excellent V <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">oc</sub> of 743 mV at the R&D stage. The accomplishment of the 22.8% cell demonstrates that HIT solar cells are advantageous to the use of thinner Si wafers because of certain HIT solar cell features.
1,2,4‐Benzothiadiazine Derivatives as α1 and 5‐HT1A Receptor Ligands. (2005)

Abstract For Abstract see ChemInform Abstract in Full Text.
Interstitial Cell Remodeling Promotes Aberrant Adipogenesis in Dystrophic Muscles (2020)

Fibrosis and fat replacement in skeletal muscle are major complications that lead to a loss of mobility in chronic muscle disorders, such as muscular dystrophy. However, the in vivo properties of adipogenic stem and precursor cells remain unclear, mainly due to the high cell heterogeneity in skeletal muscles. Here, we use single-cell RNA sequencing to decomplexify interstitial cell populations in healthy and dystrophic skeletal muscles. We identify an interstitial CD142-positive cell population in mice and humans that is responsible for the inhibition of adipogenesis through GDF10 secretion. Furthermore, we show that the interstitial cell composition is completely altered in muscular dystrophy, with a near absence of CD142-positive cells. The identification of these adipo-regulatory cells in the skeletal muscle aids our understanding of the aberrant fat deposition in muscular dystrophy, paving the way for treatments that could counteract degeneration in patients with muscular dystrophy.
Role of Raman Microprobe Spectroscopy in the Characterization of Microelectronic Materials (2001)

Abstract The production of semiconductor chips and packaging materials involves the use of a wide array of materials, from solvents and polymers, to photoresists, to metal and dielectric layers, to conductive and thermal pastes. Characterization of these materials, both in raw form and as formulated for in-process use, is integral to successful use of them in microelectronic manufacturing. Physical and chemical analytical techniques are employed to determine parameters such as composition, cure state, and interface chemistry. More often than not, it is the successful combination of complementary analyses which provide the complete understanding of material parameters needed. This paper reports the use of Raman microprobe spectroscopy as a characterization technique for microelectronic materials. Several examples will be given, illustrating the type of information which can be obtained and the complementary use of Raman spectroscopy in conjunction with other analytical techniques such as Fourier transform infrared spectroscopy (FTIR) and x-ray photoelectron spectroscopy (XPS) to characterize microelectronic packaging materials and organic materials used in semiconductor manufacture.
Effects of STAU1/staufen1 on autophagy in neurodegenerative diseases (2023)

The double-stranded RNA-binding protein, STAU1 (staufen double-stranded RNA binding protein 1) is a multifunctional protein that localizes to stress granules (SGs). We had previously found that STAU1 is overabundant in fibroblast cell lines from patients with spinocerebellar ataxia type 2 (SCA2) or amyotrophic lateral sclerosis (ALS)-frontotemporal dementia (FTD) as well as in animal models of these diseases. STAU1 overabundance is post-transcriptional and associated with MTOR hyperactivation and links SG formation with macroautophagy/autophagy. Reducing STAU1 levels in mice with ALS mutations normalizes MTOR activity and autophagy-related marker proteins. We also see increased STAU1 levels in HEK293 cells expressing C9orf72-relevant dipeptide repeats (DPRs), and DPRs are not observed in cells where <i>STAU1</i> is targeted by RNAi. Overexpression of STAU1 in HEK293 cells increases MTOR translation by directly interacting with the <i>MTOR</i> mRNA 5'UTR, activating downstream targets and impairing autophagic flux. STAU1 may constitute a novel target to modulate MTOR activity and autophagy and for the treatment of neurodegenerative diseases.
Optimized Ag interference filters for RGB and RGBIR imaging (2016)

The design of multilayer Fabry-Perot filters is optimized for colorimetric imaging. Ag/dielectric filters show performances close to the latest generation of organic resists. In addition, they can provide near-infrared detection functionality.