gebhart/patrepeval huggingface dataset:
Real‐time length measurement of epidural Tuohy needle during insertion (2013)

This study presents a novel technique for the measurement of insertion depth of an epidural Tuohy needle in real‐time. A wireless camera is used which transmits video images during insertion to a host computer. The computer contains the image processing algorithm to detect the visible needle in the image and measures the length. The measurement is done by HSV background removal, colour comparison and using RGB intensity profile to locate 10 mm markings on the Tuohy needle shaft. The visible length is then subtracted from the known length of the needle to calculate the depth of the needle tip. The camera can be placed in the operating theatre between 50 and 100 cm away from the needle insertion site. Wireless camera is beneficial since it minimises disturbance to the anaesthetist and the patient and avoids ethical and sterility concerns which would be caused by bringing additional equipment into the hospital room. The speed of the image processing technique is 10 frames per second with a maximum error of ±3 mm. The image processing failure rate was 3 frames out of 150 which gave an overall reliability of 97.8% during insertion. Also this enables continuous needle depth monitoring during insertion by storing measurements into a data file. The purpose of measuring needle depth in real time is to precisely place the needle in the epidural space.
Aminopeptidase P3 (APP3), a novel member of the TNF/TNFR2 signaling complex, induces phosphorylation of JNK (2015)

Tumor necrosis factor (TNF) is an important mediator that triggers onset of autoimmune diseases and exerts its biological effects by interacting through two receptors, TNFR1 (also known as TNFRSF1A) and TNFR2 (also known as TNFRSF1B). TNFR2 signaling has significant potential to exert pro-survival and protective roles in several diseases. Unlike TNFR1 signaling, however, the mechanism of TNFR2 signal transduction is poorly understood, and few of its adaptor molecules are known. The present study utilized a proteomics approach to search for adaptor molecules in the TNFR2 signaling complex and identified aminopeptidase P3 (APP3, also known as XPNPEP3) to be a key molecule. One of its two isoforms, mitochondrial APP3 (APP3m) but not cytosolic APP3 (APP3c), was recruited to TNFR2 and shown to regulate TNF-TNFR2-dependent phosphorylation of JNK1 (also known as MAPK8) and JNK2 (also known as MAPK9). Furthermore, APP3m was released from mitochondria upon TNF stimulation in the absence of mitochondrial outer membrane permeabilization (MOMP). The observation of increased cell death upon downregulation of APP3m also suggested that APP3m exerts an anti-apoptotic function. These findings reveal that APP3m is a new member of the TNF-TNFR2 signaling complex and characterize an APP3-mediated TNFR2 signal transduction mechanism that induces activation of JNK1 and JNK2.
Construction, characterization, and complementation of Rhodospirillum rubrum puf region mutants (1991)

Rhodospirillum rubrum is a facultatively phototrophic bacterium that, under certain growth conditions, forms an intracytoplasmic chromatophore membrane (ICM) housing the photochemical apparatus. The puf operon of R. rubrum encodes protein subunits of the photochemical reaction center and the B880 light-harvesting antenna complex. Mutant strains of R. rubrum were constructed by interposon mutagenesis through which a kanamycin resistance gene cartridge was inserted into restriction sites and in place of restriction fragments of the puf region. Southern blot analysis demonstrated that the defective copies of puf sequences had replaced their normal chromosomal counterparts through homologous recombination. The phenotypes of the mutant strains were evaluated on the basis of puf gene expression, spectral analysis, pigment content of membranes, and electron-microscopic examination of thin sections of cells grown under semi-aerobic and dark anaerobic conditions. Alterations of the puf region affect phototrophic competence and the formation of the ICM. The latter result implies an obligatory role for puf gene products in ICM formation in R. rubrum. One mutant with a deletion in puf structural genes was complemented in trans to the wild-type phenotype. Other mutants could be restored to the wild-type phenotype only by recombination.
Effect of nanoparticle clustering on the effective thermal conductivity of concentrated silica colloids (2010)

Recent research on nanofluids has offered particle clustering as a possible mechanism for the abnormal enhancement of the effective thermal conductivity (k) when nanoparticles are dispersed in liquids. This paper was devoted to verify experimentally and theoretically the significance of the effect by altering the cluster structure, size distribution, and thermal conductivity of solid particles in water. Starting with well dispersed SiO2 sols in water as a reference system, we control the aggregation kinetics by adjusting pH. Contrary to previous model predictions, the present experiment showed that clustering did not show any discernable enhancement in the thermal conductivity even at high volume loading. A series of fractal model calculations not only suggested that the conductive benefit due to clustering might be completely compensated by the reduced convective contribution due to particle growth, but also recommended the need for higher thermal conductivity and optimized fractal dimension of particles for maximizing the clustering effect.
Effect of Wheat Herbicide Carryover on Double-Crop Cotton and Soybean (2012)

In the southeastern United States many farmers double-crop winter wheat with soybean or cotton. However, there is little information about residual injury of herbicides used in wheat to these rotational crops. Experiments were conducted from 2007 to 2008 and 2008 to 2009 in soft red winter wheat to evaluate response of rotational crops of soybean and cotton after application of various acetolactate synthase herbicides in wheat. Pyroxsulam, mesosulfuron, sulfosulfuron, propoxycarbazone, or chlorsulfuron plus metsulfuron at multiple rates were applied to wheat approximately 110 to 120 d before planting rotational crops. Soils were Tift loamy sand at Ty Ty, GA and Faceville sandy loam at Plains, GA. After wheat harvest, soybean (‘Pioneer 97M50’) and cotton (‘DP 0949 B2RF’) were strip-tillage planted and evaluated for injury, stand density, height over time, and yields. For both locations, wheat was tolerant to all herbicide treatments with little to no visible injury 7 to 90 d after application. Pyroxsulam injury was less than sulfosulfuron or mesosulfuron. At recommended use rates, wheat injury was transient with no effect on yield. Double-crop soybean for both locations had no differences in stand establishment for any herbicide treatments. There was significant carryover injury to soybean and cotton for sulfosulfuron applied to wheat for the Faceville sandy loam. There was no effect of herbicide treatment on cotton stand. There was little to no difference in residual activity on rotational crops between pyroxsulam and other wheat herbicides when labeled rates were applied. This is significant as pyroxsulam is used to control Italian ryegrass and wild radish in this region.
A phase Ib study to evaluate the PI3-kinase inhibitor GDC-0941 with paclitaxel (P) and carboplatin (C), with and without bevacizumab (BEV), in patients with advanced non-small cell lung cancer (NSCLC). (2011)

3044 Background: Genetic alterations affecting the PI3K pathway are frequently observed in NSCLC (eg. PTEN loss, PIK3CA gene amplification). In preclinical tumor models GDC-0941 is synergistic with chemotherapy and increases the efficacy of anti-VEGF when dosed concurrently. This study sought to determine whether GDC-0941 can be safely combined with P + C +/- BEV. Methods: In Arm A, BEV-ineligible patients (pts) with advanced NSCLC and 0 or 1 prior chemotherapy regimen received increasing GDC-0941 doses (3 + 3 design) with P (200 mg/m2) and C (AUC 6 mg/mL·min) every 3 weeks. BEV-eligible pts in Arm B received BEV (15 mg/kg) in addition to P and C. In both arms, GDC-0941 was given once daily on Days 1-14 of a 21-day cycle. P and C were given up to 6 cycles, while GDC-0941 (with or without BEV) was given to progression or toxicity. Study objectives were to evaluate safety and pharmacokinetics (PK), and to determine the maximum tolerated dose (MTD) of GDC-0941 in both arms. Results: As of 9 January 2011, 18 pts were enrolled in GDC-0941 cohorts of 60, 100, and 165 mg (Arm A) and GDC-0941 cohorts of 100, 165 and 250 mg (Arm B). Eleven of 18 pts were active. Treatment-related adverse events (AEs) in ≥20% of pts were alopecia, asthenia, nausea, stomatitis, neutropenia, decreased appetite, paresthesia, epistaxis, arthralgia, peripheral neuropathy and rash. All treatment-related AEs were Grade 1 and 2 except for neutropenia. Grade 3 (16%) and 4 (11%) neutropenia events were not dose-limiting. PK characteristics of GDC-0941, P, 6-OH-P and C were similar to historical single-agent profiles. Based on preclinical efficacy, target exposures were achieved in the 250 mg cohort. Dose escalation will continue to a maximum of 400 mg. Confirmed partial responses (PR) were seen in 8 of 18 (44%) patients, including 1 squamous NSCLC pt with a pathologic complete response upon resection of the primary lung lesion (Arm A, 165 mg GDC-0941). Median follow up at the time of data cut-off was 5.4 months (range 0.6-10.3 m). Conclusions: The combination of GDC-0941, P and C (+/- BEV) was well tolerated. The combination MTD has not yet been reached. Antitumor activity was observed.
Localization of Phosphorylated Serine, Osteopontin, and Bone Sialoprotein on Bone Fracture Surfaces (2009)

Bone mineral density or bone mass alone cannot reliably predict fracture risk in patients. It is generally accepted that bone quality, including the properties of the organic matrix of bone, should also be considered. Collagen type I accounts for about 90% of this organic matrix. The other 10% are accounted for by various proteins and proteoglycans usually summarized by the term noncollagenous proteins (NCPs). These NCPs have a large influence on the nanoscale organization of bone. In addition, some NCPs have intriguing properties that could strongly influence bone matrix material properties; they can form self-healing networks based on ion-mediated bonds. Such behavior was also reported for trabecular bone fracture surfaces, rejoined after cleavage. To obtain proof that this behavior of bone is due to NCPs, an immunohistochemical approach was chosen for the work presented in this communication. Antibodies for phosphoserine, which is abundant in many NCPs but not in collagen type I, as well as antibodies for osteopontin and bone sialoprotein, were used on human trabecular bone fracture surfaces and microfractured trabeculae. Signals were detected using secondary gold-labeled antibodies and backscattered scanning electron microscopy. We found homogenous NCP coverage of fracture surfaces and elevated signals on bridging ligaments. Osteopontin and bone sialoprotein were detected in localized patches. Overall, this work suggests that the self-healing effect of trabecluar bone fracture surfaces, rejoined after cleavage, can be explained by the presence of NCPs. In addition, we conclude that NCPs also constitute the interface that is disrupted when bone fails, attributing them high importance for bone matrix material properties and fracture risk.
A field application of LOD (2012)

Semantic search is intrinsically suited for information retrieval in field research, where keyword search is burdensome for users. Also, exploitation of mobile and facility sensors is now prevailing, but applications are still vague. Therefore, LOD can serve as an intermediary interpreting the semantics of the users and their environmental information obtained by the sensors and connecting it to the collective intelligence on the net. In this paper, to build ecosystem of LOD used in the field, we propose a mechanism of LOD content generation and its field application. Firstly, we introduce an Android application, which searches a plant fitting the environmental conditions obtained by the sensors from Plant LOD. Then, we show that our LOD generation mechanism builds the Plant LOD from the Web with an accuracy of 85 to 97%.
Task-based Assessment and Optimization of Gamma-ray Imaging Systems (2005)

Multiple-pinhole gamma-ray imaging systems for use in nuclear medicine are discussed. Criteria and approaches for optimizing the systems for specific clinical tasks and specific patients are presented.
SEM Observations of Nickel-Titanium Rotary Endodontic Instruments that Fractured During Clinical Use (2004)

Numerous discarded ProFile GT, ProFile, and ProTaper nickel-titanium rotary instruments obtained from two graduate endodontic clinics were examined by scanning electron microscopy. These instruments had an unknown history of clinical use and had fractured or experienced considerable permanent torsional deformation without complete separation. The failure processes generally exhibited substantial ductile character, evidenced by a dimpled rupture fracture surface. Crack propagation at grain boundaries and cleavage surfaces indicative of transgranular fracture were observed for some specimens. It appeared that oxide particles from the manufacturing process served as nucleating sites for the microvoids, leading to dimpled rupture. A previously unreported fracture mode also was observed, in which crack propagation, approximately parallel to the local flute orientation, connected pitted regions on the surface. Combining present and previous scanning electron microscopy observations of clinically failed instruments, suggestions are offered for improving their fracture resistance.
ras activation in human tumors and in animal model systems. (1991)

Environmental agents such as radiation and chemicals are known to cause genetic damage. Alterations in a limited set of cellular genes called proto-oncogenes lead to unregulated proliferation and differentiation. We have studied the role of the ras gene family in carcinogenesis using two different animal models. In one case, thymic lymphomas were induced in mice by either gamma or neutron radiation, and in the other, keratoacanthomas were induced in rabbit skin with dimethylbezanthracene. Human keratoacanthomas similar to the ones induced in rabbits were also analyzed. We found that different types of radiation such as gamma rays and neutrons, induced different point mutations in ras genes. A novel K-ras mutation in codon 146 has been found in thymic lymphomas induced by neutrons. Keratoacanthomas induced in rabbit skin by dimethylbenzanthracene show a high frequency of H-ras-activated genes carrying a mutation in codon 61. The same is observed in human keratoacanthomas, although mutations are in both the 12th and the 61st codons of the H-ras gene. H-ras activation is less frequent in human squamous cell carcinomas than in keratoacanthomas, suggesting that ras genes could play a role in vivo in differentiation as well as in proliferation.
Trehalose Synthesis by Sequential Reactions of Recombinant Maltooligosyltrehalose Synthase and Maltooligosyltrehalose Trehalohydrolase from Brevibacterium helvolum (2000)

A DNA fragment encoding two enzymes leading to trehalose biosynthesis, maltooligosyltrehalose synthase (BvMTS) and maltooligosyltrehalose trehalohydrolase (BvMTH), was cloned from the nonpathogenic bacterium Brevibacterium helvolum. The open reading frames for the two proteins are 2,331 and 1,770 bp long, respectively, and overlap by four nucleotides. Recombinant BvMTS, BvMTH, and fusion gene BvMTSH, constructed by insertion of an adenylate in the overlapping region, were expressed in Escherichia coli. Purified BvMTS protein catalyzed conversion of maltopentaose to maltotriosyltrehalose, which was further hydrolyzed by BvMTH protein to produce trehalose and maltotriose. The enzymes shortened maltooligosaccharides by two glucose units per cycle of sequential reactions and released trehalose. Maltotriose and maltose were not catalyzed further and thus remained in the reaction mixtures depending on whether the substrates had an odd or even number of glucose units. The bifunctional in-frame fusion enzyme, BvMTSH, catalyzed the sequential reactions more efficiently than an equimolar mixture of the two individual enzymes did, presumably due to a proximity effect on the catalytic sites of the enzymes. The recombinant enzymes produced trehalose from soluble starch, an abundant natural source for trehalose production. Addition of alpha-amylase to the enzyme reaction mixture dramatically increased trehalose production by partial hydrolysis of the starch to provide more reducing ends accessible to the BvMTS catalytic sites.
Automated characterization of gene expression patterns with an atlas of the mouse brain (2005)

A spatio-temporal map of gene activity in the brain would be an important contribution to the understanding of brain development, disease, and function. Such a resource is now possible using high-throughput in situ hybridization, a method for transcriptome-wide acquisition of cellular resolution gene expression patterns in serial tissue sections. However, querying an enormous quantity of image data requires computational methods for describing and organizing gene expression patterns in a consistent manner. In addressing this, we have developed procedures for automated annotation of gene expression patterns in the postnatal mouse brain.
Modeling and Implementation of Subthreshold Characteristics of Accumulation-Mode MOSFETs for Various SOI Layer Thickness and Impurity Concentrations (2007)

This work reports on the modeling of the unique subthreshold characteristics of the AM-MOSFETs and the implementation to the device to control the subthreshold characteristics and electrical stress immunity. Subthreshold characteristics degradation due to hot carrier stress can be significantly suppressed by tuning the device to the bulk current controlled device. Moreover, scalability of the accumulation-mode MOSFETs becomes comparable to inversion- and intrinsic-mode MOSFETs for ultra-thin T <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">SOI</sub> even for the deep sub-100 nm regime.
Synthesis of Aromatase Inhibitors and Dual Aromatase Steroid Sulfatase Inhibitors by Linking an Arylsulfamate Motif to 4‐(4H‐1,2,4‐triazol‐4‐ylamino)benzonitrile: SAR, Crystal Structures, in vitro and in vivo Activities (2008)

4-(((4-Cyanophenyl)(4H-1,2,4-triazol-4-yl)amino)methyl)phenyl sulfamate (6 a) was the first dual aromatase-sulfatase inhibitor (DASI) reported. Several series of its derivatives with various linker systems between the steroid sulfatase (STS) and the aromatase inhibitory pharmacophores were synthesised and evaluated in JEG-3 cells. The X-ray crystal structures of the aromatase inhibitors, DASI precursors 42 d and 60, and DASI 43 h were determined. Nearly all derivatives show improved in vitro aromatase inhibition over 6 a but decreased STS inhibition. The best aromatase inhibitor is 42 e (IC(50)=0.26 nM) and the best DASI is 43 e (IC(50 aromatase)=0.45 nM, IC(50 STS)=1200 nM). SAR for aromatase inhibition shows that compounds containing an alkylene- and thioether-based linker system are more potent than those that are ether-, sulfone-, or sulfonamide-based, and that the length of the linker has a limited effect on aromatase inhibition beyond two methylene units. Compounds 43 d-f were studied in vivo (10 mg kg(-1), single, p.o.). The most potent DASI is 43 e, which inhibited PMSG-induced plasma estradiol levels by 92 % and liver STS activity by 98 % 3 h after dosing. These results further strengthen the concept of designing and developing DASIs for potential treatment of hormone-related cancers.
Characterizing ventricular mechanics and energetics following repeated coronary microembolization (1997)

Myocardial mechanics and energetics were investigated in an animal model of moderate chronic heart failure (CHF) created by repeated coronary microembolizations in six dogs. The final fractional area change was 34 +/- 4%. Hearts of these animals were isolated and cross-perfused, and balloons were placed in the left ventricle (LV). Chamber contractile state was markedly depressed in embolized hearts as assessed by the slope (Ees 2.74 +/- 0.49 vs. 4.00 +/- 1.18 mmHg/ml, P < 0.01) and volume axis intercept (V: 8.7 +/- 5.9 vs. 1.0 +/- 3.2 ml, P < 0.01) of end-systolic pressure-volume relation compared with a group of six normal dogs. The end-diastolic pressure-volume relation of embolized hearts was shifted to the right, indicating a dilation of the LV. However, systolic and diastolic stress strain relationships were similar in the two groups, suggesting that the average myocardial properties of the embolized hearts are similar to those of normal hearts. The relationship between oxygen consumption and pressure-volume area in embolized hearts had smaller intercept (2.98 +/- 0.44 vs 3.92 +/- 0.39 x 10(-2) ml O2.beat-1.100 g LV-1, P < 0.01) compared with the control group, with no change in the slope. These results contrast with previous findings in pacing CHF and serve as an important characterization of ventricular properties in this model of CHF from different etiology.
Evidence for heritability of non-modulating essential hypertension. (1989)

We have previously described a subset of subjects with essential hypertension who fail to appropriately modulate renal vascular and adrenal reactivity with changes in dietary sodium and in response to infused angiotensin II (Ang II). In this paper, we studied these responses in 13 unselected hypertensive subjects in whom the family history of hypertension had been carefully detailed. Nine of these 13 subjects had a positive family history (FH+) for hypertension and had significantly smaller decrements in renal blood flow with Ang II infusion than the four subjects who had a negative family history (FH-) (-84 +/- 16 ml/min/1.73 m2 for FH+ vs. -149 ml/min/1.73 m2 for FH-, p = 0.024). These FH+ subjects also showed smaller increases in renal blood flow with increases in dietary sodium than FH- subjects (7 +/- 10 ml/min/1.73 m2 vs. 72 +/- 24 ml/min/1.73 m2, respectively; p = 0.014). When classified as modulators or non-modulators by previously established criteria, all seven non-modulators were FH+, and seven of nine FH+ subjects were non-modulators. This association between non-modulation and family history of hypertension is significant (p = 0.021). To further clarify the association between non-modulation and family history of hypertension, we have studied the renal blood flow response to Ang II in 31 hypertensive siblings from 14 sibships. Twenty-five of these 31 subjects (81%) behaved as non-modulators (p = 0.008 compared with expected value in an unselected hypertensive population). Additionally, strong concordance of non-modulation between sibling pairs was observed (p = 0.004).(ABSTRACT TRUNCATED AT 250 WORDS)