gebhart/patrepeval huggingface dataset:
Correlation between the reliability of HEMT devices and that of a combined oscillator-amplifier MMIC (2001)

Simultaneous therapy and regeneration of bone tumor-induced defects still remain to be a significant challenge. Conventional therapy strategy by implanting bone graft materials can regenerate the bone defects after surgery but cannot kill residual tumor cells. In this study, we successfully prepared a 3D-printed β-tricalcium phosphate bioceramic scaffold with surface modification of Fe<sub>3</sub>O<sub>4</sub> nanoparticles/graphene oxide nanocomposite layers (named β-TCP-Fe-GO). The prepared β-TCP-Fe-GO scaffolds possess a highly ordered macroporous structure with triangle pore morphology and a pore size of around 300-500 μm. The struts of β-TCP-Fe-GO scaffolds were uniformly deposited with Fe<sub>3</sub>O<sub>4</sub>/GO sandwich-like composite layers in which nano-sized Fe<sub>3</sub>O<sub>4</sub> particles were wrapped by GO sheets. The Fe<sub>3</sub>O<sub>4</sub> content in the β-TCP-Fe-GO scaffolds can be effectively modulated by controlling the coating times; the final content of Fe<sub>3</sub>O<sub>4</sub> in β-TCP-8Fe-GO scaffolds is no more than 1% after coating 8 times. Such low content of Fe<sub>3</sub>O<sub>4</sub> in the scaffolds endows them with super paramagnetic behavior and hyperthermal effects. The temperature of the scaffolds can be modulated in the range 50-80 °C under an alternating magnetic field for 15 minutes by controlling the magnetic intensity and Fe<sub>3</sub>O<sub>4</sub> content. The excellent hyperthermal effect of β-TCP-Fe-GO scaffolds induced more than 75% cell death for osteosarcoma cells (MG-63) in vitro. Furthermore, the β-TCP-Fe-GO scaffolds significantly enhanced alkaline phosphatase (ALP) activity and osteogenic gene expression, such as OPN, Runx2, OCN and BSP, of rabbit bone marrow stromal cells (rBMSCs) and significantly stimulated rBMSCs proliferation as compared to pure β-TCP scaffolds by the synergistic effect of GO and the released Fe ions. Therefore, the prepared β-TCP-Fe-GO scaffolds possess prominent magnetothermal ability and excellent bone-forming activity. This study is believed to pave the way for the design and fabrication of novel tissue engineering scaffolds in a combination of therapy and regeneration functions.
Dopamine 5 receptor mediates Ang II type 1 receptor degradation via a ubiquitin-proteasome pathway in mice and human cells (2008)

Hypertension is a multigenic disorder in which abnormal counterregulation between dopamine and Ang II plays a role. Recent studies suggest that this counterregulation results, at least in part, from regulation of the expression of both the antihypertensive dopamine 5 receptor (D5R) and the prohypertensive Ang II type 1 receptor (AT1R). In this report, we investigated the in vivo and in vitro interaction between these GPCRs. Disruption of the gene encoding D5R in mice increased both blood pressure and AT1R protein expression, and the increase in blood pressure was reversed by AT1R blockade. Activation of D5R increased the degradation of glycosylated AT1R in proteasomes in HEK cells and human renal proximal tubule cells heterologously and endogenously expressing human AT1R and D5R. Confocal microscopy, Förster/fluorescence resonance energy transfer microscopy, and fluorescence lifetime imaging microscopy revealed that activation of D5R initiated ubiquitination of the glycosylated AT1R at the plasma membrane. The regulated degradation of AT1R via a ubiquitin/proteasome pathway by activation of D5R provides what we believe to be a novel mechanism whereby blood pressure can be regulated by the interaction of 2 counterregulatory GPCRs. Our results therefore suggest that treatments for hypertension might be optimized by designing compounds that can target the AT1R and the D5R.
ChemInform Abstract: The Case Favoring Direct C‐Alkylation of Heteroatom Substituted Enolates. (1989)

Abstract Lithium dimethylcuprate (II) reacts with the benzylselenenylcyclopentenone (I) to give an enolate which undergoes direct C‐alkylation with the halides (III), producing a mixture of the isomeric cyclopentanones (IV) and (V).
Semantic characterization of MapReduce workloads (2013)

MapReduce is a platform for analyzing large amounts of data on clusters of commodity machines. MapReduce is popular, in part thanks to its apparent simplicity. However, there are unstated requirements for the semantics of MapReduce applications that can affect their correctness and performance. MapReduce implementations do not check whether user code satisfies these requirements, leading to time-consuming debugging sessions, performance problems, and, worst of all, silently corrupt results. This paper makes these requirements explicit, framing them as semantic properties and assumed outcomes. It describes a black-box approach for testing for these properties, and uses the approach to characterize the semantics of 23 non-trivial MapReduce workloads. Surprisingly, we found that for most requirements, there is at least one workload that violates it. This means that MapReduce may be simple to use, but it is not as simple to use correctly. Based on our results, we provide insights to users on how to write higher-quality MapReduce code, and insights to system and language designers on ways to make their platforms more robust.
Structural basis of ligand interaction with atypical chemokine receptor 3 (2017)

Chemokines drive cell migration through their interactions with seven-transmembrane (7TM) chemokine receptors on cell surfaces. The atypical chemokine receptor 3 (ACKR3) binds chemokines CXCL11 and CXCL12 and signals exclusively through β-arrestin-mediated pathways, without activating canonical G-protein signalling. This receptor is upregulated in numerous cancers making it a potential drug target. Here we collected over 100 distinct structural probes from radiolytic footprinting, disulfide trapping, and mutagenesis to map the structures of ACKR3:CXCL12 and ACKR3:small-molecule complexes, including dynamic regions that proved unresolvable by X-ray crystallography in homologous receptors. The data are integrated with molecular modelling to produce complete and cohesive experimentally driven models that confirm and expand on the existing knowledge of the architecture of receptor:chemokine and receptor:small-molecule complexes. Additionally, we detected and characterized ligand-induced conformational changes in the transmembrane and intracellular regions of ACKR3 that elucidate fundamental structural elements of agonism in this atypical receptor.
Supplementary Materials, Legends for Figures 1-5 from Efficacy of Levo-1-Methyl Tryptophan and Dextro-1-Methyl Tryptophan in Reversing Indoleamine-2,3-Dioxygenase–Mediated Arrest of T-Cell Proliferation in Human Epithelial Ovarian Cancer (2023)

Supplementary Materials, Legends for Figures 1-5 from Efficacy of Levo-1-Methyl Tryptophan and Dextro-1-Methyl Tryptophan in Reversing Indoleamine-2,3-Dioxygenase–Mediated Arrest of T-Cell Proliferation in Human Epithelial Ovarian Cancer
<title>Wind tunnel model aircraft attitude and motion analysis</title> (1991)

A model-based approach to estimating the position and pitch-roll-yaw of the model aircraft in a vertical wind tunnel used to study the aircraft spin characteristics. The video image sequence of the model during flight is digitally analyzed for detection and tracking of target markers installed on the model. The geometric model of these targets is represented by their coordinates relative to the model reference coordinates. The six degrees of freedom (six DOF), namely, the pitch-roll-yaw and the 3-D position, is estimated using the camera model and iterative minimization of the distance in the image plane between the target points and the projected model points. Using prediction of the six DOF for the next video frame, the estimation procedure is repeated in a six DOF tracking loop that utilizes the dynamic as well as the geometric model of the target. The six DOF estimation and target tracking has been implemented on a real-time image processing workstation based on the Sun SPARC architecture. Interactive graphics and video image processing is used for initial designation of the approximate six DOF by the operator, followed by automatic tracking which dynamically displays the stick figure of the model aircraft on the grey-level video sequence.
Fuel Cell Stack Emulation for Cell and Hardware-in-the-Loop Testing (2018)

The high costs of fuel cell stacks are often prohibitive for research applications. Instead, researchers tend to either perform tests on individual cells under emulated load conditions, or they use model-based emulated fuel cell stacks to test realistic loads. This paper presents an alternative technique that allows the simultaneous investigation of both cell and hardware. In this technique, an amplifier takes as input the voltage of a reference fuel cell and reproduces the “scaled-up” voltage that would be provided by a stack of similar cells. The output terminals of this simulated stack can be connected to realistic loads, which are series connected with the reference cell to maintain a one-to-one correspondence of cell and load currents. This technique allows the simultaneous investigation of stack/load interactions, stack electrochemical performance characteristics, and stack durability under load. The amplifier electronics are independent of the fuel cell allowing its use with all fuel cell types, e.g., solid oxide fuel cells (SOFCs) or proton exchange membrane fuel cells. We demonstrate the emulator using a single planar SOFC and under several real loads. The data collected reveal good steady-state and dynamic accuracy, and the observed electrical interactions highlight the utility of the emulator in cell and hardware-in-the-loop testing. Ultimately, this technique provides fuel cell system researchers and developers a low-cost, easily implemented tool for rapid assessment of novel fuel cell technologies under real-world loading conditions.
Electric-field fluctuations in random dielectric composites (1997)

When a composite is subjected to a constant applied electric, thermal, or stress field, the associated local fields exhibit strong spatial fluctuations. In this paper, we evaluate the distribution of the local electric field (i.e., all moments of the field) for continuum (off-lattice) models of random dielectric composites. The local electric field in the composite is calculated by solving the governing partial differential equations using efficient and accurate integral equation techniques. We consider three different two-dimensional dispersions in which the inclusions are either (i) circular disks, (ii) squares, or (iii) needles. Our results show that in general the probability density function associated with the electric field for disks and squares exhibits a double-peak character. Therefore, the variance or second moment of the field is inadequate in characterizing the field fluctuations in the composite. Moreover, our results suggest that the variances for each phase are generally not equal to each other. In the case of a dilute concentration of needles, the probability density function is a singly peaked one, but the higher-order moments are appreciably larger for needles than for either disks or squares.
Progress in actinic mask imaging: the closest look at lines, defects in phase roughness (2010)

Author(s): Goldberg, Kenneth A.; Mochi, Iacopo; Naulleau, Patrick; George, Simi; Huh, Sungmin
Supplementary Figure 7 from Identification and Characterization of NVP-BKM120, an Orally Available Pan-Class I PI3-Kinase Inhibitor (2023)

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Functional analysis of a chromosomal passenger protein, Borealin, by multiphoton excitation-evoked chromophore-assisted laser inactivation (2009)

Borealin, one of the chromosomal passenger proteins, is reported to play important roles in the mitotic process. To analyze precise functions of Borealin in the mitotic phase, we applied the method of multiphoton excitation-evoked chromophore-assisted laser inactivation. Although further studies are needed, our results suggest that Borealin is required for mitosis, as previously reported. These results provide insights into understanding the functions of chromosomal passenger proteins during mitosis.
Evaluating window-wall interface details for risk of condensation on windows (2009)

The paper reports on a laboratory evaluation of the formation of condensation at the window frame perimeter of the interface assembly as a function of both temperature deferential and air leakage rate across the test assembly. A summary of the laboratory test protocol is provided that includes a description of the test set-up and apparatus, fabrication details of the specimen and information on instrumentation and calibration. Results on the potential for the formation of condensation at the window are given in terms of temperature differentials as well as specific air leakage paths and rates of air leakage across the specimen.
Enhanced Stability of Pt-TiO 2 -CNT Heterostructure Composite Cathodes for Li-O 2 Batteries Studied by High-Resolution AEM (2016)

Journal Article Enhanced Stability of Pt-TiO 2 -CNT Heterostructure Composite Cathodes for Li-O 2 Batteries Studied by High-Resolution AEM Get access VP Oleshko, VP Oleshko Material Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD,USA Search for other works by this author on: Oxford Academic Google Scholar M Noked, M Noked Department of Chemistry and Biochemistry and Search for other works by this author on: Oxford Academic Google Scholar MA Schroeder, MA Schroeder Department of Materials Science and Engineering, University of Maryland, College Park, MD, USA Search for other works by this author on: Oxford Academic Google Scholar C Liu, C Liu Department of Materials Science and Engineering, University of Maryland, College Park, MD, USA Search for other works by this author on: Oxford Academic Google Scholar AJ Pearse, AJ Pearse Department of Materials Science and Engineering, University of Maryland, College Park, MD, USA Search for other works by this author on: Oxford Academic Google Scholar SB Lee, SB Lee Department of Chemistry and Biochemistry and Search for other works by this author on: Oxford Academic Google Scholar CL Soles, CL Soles Material Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD,USA Search for other works by this author on: Oxford Academic Google Scholar GW Rubloff GW Rubloff Department of Materials Science and Engineering, University of Maryland, College Park, MD, USA Search for other works by this author on: Oxford Academic Google Scholar Microscopy and Microanalysis, Volume 22, Issue S3, 1 July 2016, Pages 1314–1315, https://doi.org/10.1017/S1431927616007418 Published: 25 July 2016
A single-cell map of dynamic chromatin landscapes of immune cells in renal cell carcinoma (2022)

A complete chart of the chromatin regulatory elements of immune cells in patients with cancer and their dynamic behavior is necessary to understand the developmental fates and guide therapeutic strategies. Here, we map the single-cell chromatin landscape of immune cells from blood, normal tumor-adjacent kidney tissue and malignant tissue from patients with early-stage clear cell renal cell carcinoma (ccRCC). We catalog the T cell states dictated by tissue-specific and developmental-stage-specific chromatin accessibility patterns, infer key chromatin regulators and observe rewiring of regulatory networks in the progression to dysfunction in CD8<sup>+</sup> T cells. Unexpectedly, among the transcription factors orchestrating the path to dysfunction, NF-κB is associated with a pro-apoptotic program in late stages of dysfunction in tumor-infiltrating CD8<sup>+</sup> T cells. Importantly, this epigenomic profiling stratified ccRCC patients based on a NF-κB-driven pro-apoptotic signature. This study provides a rich resource for understanding the functional states and regulatory dynamics of immune cells in ccRCC.
RABBIT PLATELET BACTERICIDAL PROTEIN (1971)

The heat-stable antibacterial activity of rabbit serum against Gram-positive microorganisms has been shown to reside in a cationic protein fraction of platelet lysosomal granules. The activity is released during platelet aggregation. No plasma or serum component is required for the bactericidal effect. The platelet bactericidin resembles the antibacterial proteins of leukocyte granules both in cellular localization and in biochemical characteristics. It can be differentiated from platelet factor 4, the antiheparin factor, which is also a basic protein in platelet granules. The antibacterial effect of the platelet bactericidin may be related to the metabolic activity of the organisms. This antibacterial activity of platelets may represent another means by which platelets can participate in host inflammatory defense reactions.
Two-component order parameter for quantifying clathrate hydrate nucleation and growth (2014)

Methane clathrate hydrate nucleation and growth is investigated via analysis of molecular dynamics simulations using a new order parameter. This order parameter (OP), named the Mutually Coordinated Guest (MCG) OP, quantifies the appearance and connectivity of molecular clusters composed of guests separated by water clusters. It is the first two-component OP used for quantifying hydrate nucleation and growth. The algorithm for calculating the MCG OP is described in detail. Its physical motivation and advantages compared to existing methods are discussed.
Pseudo-nondiffracting beams generated by radial harmonic functions: erratum (1996)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text Joseph Rosen, Boaz Salik, and Amnon Yariv, "Pseudo-nondiffracting beams generated by radial harmonic functions: erratum," J. Opt. Soc. Am. A 13, 387-387 (1996) Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
Recent Progress on Tubulin Inhibitors with Dual Targeting Capabilities for Cancer Therapy (2021)

Microtubules play a crucial role in multiple cellular functions including mitosis, cell signaling, and organelle trafficking, which makes the microtubule an important target for cancer therapy. Despite the great successes of microtubule-targeting agents in the clinic, the development of drug resistance and dose-limiting toxicity restrict their clinical efficacy. In recent years, multitarget therapy has been considered an effective strategy to achieve higher therapeutic efficacy, in particular dual-target drugs. In terms of the synergetic effect of tubulin and other antitumor agents such as receptor tyrosine kinases inhibitors, histone deacetylases inhibitors, DNA-damaging agents, and topoisomerase inhibitors in combination therapy, designing dual-target tubulin inhibitors is regarded as a promising approach to overcome these limitations and improve therapeutic efficacy. In this Perspective, we discussed rational target combinations, design strategies, structure-activity relationships, and future directions of dual-target tubulin inhibitors.
Managing after-work stress: paced breathing while watching video content (abstract) (2011)

Objectives. After a hard days work, we need to recover from work stress. Two frequently reported activities to relieve stress are television (TV) watching and paced breathing exercises. We investigated the potential of combining these techniques. Method. Two explorativeexperiments were conducted. In both experiments, stress was measured through physiological monitoring (heart rate, blood pressure, skinconductance, peripheral temperature, and respiration) and the Stress Arousal Checklist [3]. All experiments ended with a semi-structured interview. The first experiment explored TV-content: free cable-TVwatching versus a dedicated relaxation video (waterfall scenery).The second experiment explored the effects of watching video content (real-life nature video and computer-generated visualizations) with- and without a visual paced breathing instruction. Results. In thefirst experiment, both conditions were reported as relaxing, but neither was sizably doing more so. The physiological results do not clearly differ between both conditions. The main finding is that different people like different content to relax; some found the TV-content not as relaxing whereas others found the dedicated relaxation content rather boring. In the second experiment participants had some difficulty following the paced breathing instruction. However, the participants who did manage to follow the instruction properly showed alarge heart rate variability. The real-life nature scenery hada stronger effect on stress reduction (self-reported) and was also preferred by most participants. Conclusion and discussion. Strong interpersonal differences exist regarding video material for relaxation. This may be due to the fact that free TV watching offered more arousing stimuli but also more control than the waterfall video. Both control over ones environment [1] and visual content [2] can impactstress levels and these effects likely differ per individual. Therefore, to be relaxing, video content should be engaging yet not too arousing and should ideally be selected by the viewer. The presented results provide directions regarding the use of video content for stress relief. Paced breathing may be a beneficial addition but furtherinvestigation is needed to e.g. find the optimal paced breathing settings and to examine possible learning effects.
Isolation and characterisation of conomap‐Vt, a d‐amino acid containing excitatory peptide from the venom of a vermivorous cone snail (2006)

Cone snail venom is a rich source of bioactives, in particular small disulfide rich peptides that disrupt synaptic transmission. Here, we report the discovery of conomap-Vt (Conp-Vt), an unusual linear tetradecapeptide isolated from Conus vitulinus venom. The sequence displays no homology to known conopeptides, but displays significant homology to peptides of the MATP (myoactive tetradecapeptide) family, which are important endogenous neuromodulators in molluscs, annelids and insects. Conp-Vt showed potent excitatory activity in several snail isolated tissue preparations. Similar to ACh, repeated doses of Conp-Vt were tachyphylactic. Since nicotinic and muscarinic antagonists failed to block its effect and Conp-Vt desensitised tissue remained responsive to ACh, it appears that Conp-Vt contractions were non-cholinergic in origin. Finally, biochemical studies revealed that Conp-Vt is the first member of the MATP family with a d-amino acid. Interestingly, the isomerization of L-Phe to D-Phe enhanced biological activity, suggesting that this post-translational modified conopeptide may have evolved for prey capture.
Gasdermins in Apoptosis: New players in an Old Game. (2019)

Apoptosis is a form of programmed cell death (PCD) that plays critical physiological roles in removing superfluous or dangerous cell populations that are unneeded or threatening to the health of the host organism. Although the molecular pathways leading to activation of the apoptotic program have been extensively studied and characterized starting in the 1970s, new evidence suggests that members of the gasdermin superfamily are novel pore-forming proteins that augment apoptosis by permeabilizing the mitochondria and participate in the final stages of the apoptotic program by inducing secondary necrosis/pyroptosis. These findings may explain outstanding questions in the field such as why certain gasdermin members sensitize cells to apoptosis, and why some apoptotic cells also show morphological features of necrosis. Furthermore, the interplay between the gasdermins and apoptosis may also explain why genetic and epigenetic alterations in these genes cause diseases and disorders like cancer and hearing loss. This review focuses on our current understanding of the function of several gasdermin superfamily members, their role in apoptosis, and how they may contribute to pathophysiological conditions.