gebhart/patrepeval huggingface dataset:
Alignment and partial structural analysis of the cyanogen bromide fragments from yeast inorganic pyrophosphatase (1974)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAlignment and partial structural analysis of the cyanogen bromide fragments from yeast inorganic pyrophosphataseRichard Sterner, Claudia Noyes, and Robert L. HeinriksonCite this: Biochemistry 1974, 13, 1, 91–99Publication Date (Print):January 1, 1974Publication History Published online1 May 2002Published inissue 1 January 1974https://pubs.acs.org/doi/10.1021/bi00698a015https://doi.org/10.1021/bi00698a015research-articleACS PublicationsRequest reuse permissionsArticle Views22Altmetric-Citations14LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Laser Doping Using Phosphorus- and Boron-Doped Silicon Nanoink (2013)

Laser doping (LD) in solar cells is becoming an increasingly promising technology and the possibility of applying LD has been studied widely for high-efficiency solar cells including passivated emitter and rear locally diffused cells with over 20% efficiency. In this work, we demonstrated phosphorus/boron laser doping using Silicon Nanoink. The Silicon Nanoink contained approximately 1×1021 atoms/cm3 of phosphorus or boron dopant. Si nanoparticle films were deposited on Si wafers by spin coating followed by laser irradiation. The doping depth was dependent on laser power and reached over 1 μm. The surface dopant concentration was approximately 1×1020 atoms/cm3 and the sheet resistance was 10 Ω/sq. We manufactured two types of solar cells and determined their cell properties.
130-Gbps DMT Transmission using Silicon Mach-Zehnder Modulator with Chirp Control at 1.55-μm (2015)

We experimentally evaluated the transmission performance of discrete multi-tone (DMT) modulation with 10-Gbps SiP MZ modulator at 1550-nm region. We achieved the transmission of more than 130-Gbps over 2-km SMF with BER of FEC limit of 3.0E-03.
Local Texture and Geometry Descriptors for Fast Block-Based Motion Estimation of Dynamic Voxelized Point Clouds (2019)

Motion estimation in dynamic point cloud analysis or compression is a computationally intensive procedure generally involving a large search space and often complex voxel matching functions. We present an extension and improvement on prior work to speed up block-based motion estimation between temporally adjacent point clouds. We introduce local, or block-based, texture descriptors as a complement to voxel geometry description. Descriptors are organized in an occupancy map which may be efficiently computed and stored. By consulting the map, a point cloud motion estimator may significantly reduce its search space while maintaining prediction distortion at similar quality levels. The proposed texture-based occupancy maps provide significant speedup, an average of 26.9% for the tested data set, with respect to prior work.
Two‐ and Three‐Terminal Resistive Switches: Nanometer‐Scale Memristors and Memistors (2011)

Abstract The logical relationship between two previously defined “memory resistors” is revealed by constructing and experimentally demonstrating a three‐terminal memistor equivalent circuit using two two‐terminal memristors. A technique is then presented, using nanoimprint lithography in combination with angle evaporation, to fabricate a single nanoscale device with a footprint of 4F 2 , where F is the minimum lithographic feature size, that can be operated as either a two‐terminal lateral memristor or a three‐terminal memistor inside a crossbar structure. These devices exhibit repeatable bipolar nonvolatile switching behavior with up to 10 3 ON/OFF conductance ratios, as well as the desired three‐terminal behavior.
Monolayer to Multilayer Nanostructural Growth Transition in N-Type Oligothiophenes on Au(111) and Implications for Organic Field-Effect Transistor Performance (2006)

The evolution in growth morphology and molecular orientation of n-type semiconducting alpha,omega-diperfluorohexyl-quaterthiophene (DFH-4T) on Au(111) is investigated by scanning tunneling microscopy and scanning tunneling spectroscopy as the film thickness is increased from one monolayer to multilayers. Monolayer-thick DFH-4T films are amorphous and morphologically featureless with a large pit density, whereas multilayer films exhibit drastically different terraced structures consisting of overlapping platelets. Large changes in DFH-4T molecular orientation are observed on transitioning from two to four monolayers. Parallel electrical characterization of top-versus-bottom contact configuration DFH-4T FETs with Au source/drain electrodes reveals greatly different mobilities (mu(TOP) = 1.1 +/- 0.2 10(-2) cm(2)V(-1)s(-1) versus mu(BOTTOM) = 2.3 +/- 0.5 10(-5) cm(2)V(-1)s(-1)) and contact resistances (R(C-TOP) = 4-12 MOmegacm vs R(C-BOTTOM) > 1 GOmegacm). This study provides important information on the organic semiconductor-source\drain electrode interfaces and explains why top-contact OFET devices typically have superior performance. By direct visualization, it demonstrates that the DFH-4T film growth transition from monolayer to multilayer on Au is accompanied by dramatic morphology and molecular orientation changes, starting from an amorphous, pitted, and disordered monolayer, to crystalline and smooth bi/tetralayers but with the molecules reoriented by 90 degrees . These chemisorption-derived inhomogenities at the contact-molecule interface and the large monolayer --> multilayer --> bulk microstructural changes are in accord with the large bottom-contact device resistance and poor OFET performance.
Seven-up Inhibits Ultraspiracle-Based Signaling Pathways In Vitro and In Vivo (1995)

Seven-up (Svp), the Drosophila homolog of the chicken ovalbumin upstream transcription factor (COUP-TF); Ultraspiracle (Usp), the Drosophila homolog of the retinoid X receptor; and the ecdysone receptor are all members of the nuclear/steroid receptor superfamily. COUP-TF negatively regulates hormonal signaling involving retinoid X receptor in tissue culture systems. Here we demonstrate that Svp, like COUP-TF, can modulate Ultraspiracle-based hormonal signaling both in vitro and in vivo. Transfection assays in CV-1 cells demonstrate that Seven-up can inhibit ecdysone-dependent transactivation by the ecdysone receptor complex, a heterodimeric complex of Usp and ecdysone receptor. This repression depends on the dose of Svp and occurs with two different Drosophila ecdysone response elements. Ectopic expression of Svp in vivo induces lethality during early metamorphosis, the time of maximal ecdysone responsiveness. Concomitant overexpression of Usp rescues the larvae from the lethal effects of Svp. DNA binding studies show that Svp can bind to various direct repeats of the sequence AGGTCA but cannot bind to one of the ecdysone response elements used in the transient transfection assays. Our results suggest that Svp-mediated repression can occur by both DNA binding competition and protein-protein interactions.
Urine as a high‐quality source of host genomic DNA from wild populations (2020)

The ability to generate genomic data from wild animal populations has the potential to give unprecedented insight into the population history and dynamics of species in their natural habitats. However, for many species, it is impossible legally, ethically or logistically to obtain tissue samples of quality sufficient for genomic analyses. In this study we evaluate the success of multiple sources of genetic material (faeces, urine, dentin and dental calculus) and several capture methods (shotgun, whole-genome, exome) in generating genome-scale data in wild eastern chimpanzees (Pan troglodytes schweinfurthii) from Gombe National Park, Tanzania. We found that urine harbours significantly more host DNA than other sources, leading to broader and deeper coverage across the genome. Urine also exhibited a lower rate of allelic dropout. We found exome sequencing to be far more successful than both shotgun sequencing and whole-genome capture at generating usable data from low-quality samples such as faeces and dental calculus. These results highlight urine as a promising and untapped source of DNA that can be noninvasively collected from wild populations of many species.
Measurement of differential tt¯ production cross sections using top quarks at large transverse momenta in pp collisions at s=13  TeV (2021)

A measurement is reported of differential top quark pair ($t\overline{t}$) production cross sections, where top quarks are produced at large transverse momenta. The data collected with the CMS detector at the LHC are from $pp$ collisions at a center-of-mass energy of $13\text{ }\text{ }\mathrm{TeV}$ corresponding to an integrated luminosity of $35.9\text{ }\text{ }{\mathrm{fb}}^{\ensuremath{-}1}$. The measurement uses events where at least one top quark decays as $t\ensuremath{\rightarrow}Wb\ensuremath{\rightarrow}q{\overline{q}}^{\ensuremath{'}}b$ and is reconstructed as a large-radius jet with transverse momentum in excess of 400 GeV. The second top quark is required to decay either in a similar way or leptonically, as inferred from a reconstructed electron or muon, a bottom quark jet, and missing transverse momentum due to the undetected neutrino. The cross section is extracted as a function of kinematic variables of individual top quarks or of the $t\overline{t}$ system. The results are presented at the particle level, within a region of phase space close to that of the experimental acceptance, and at the parton level and are compared to various theoretical models. In both decay channels, the observed absolute cross sections are significantly lower than the predictions from theory, while the normalized differential measurements are well described.
Hierarchical Mobility via Relaying in Dense Wireless Networks (2011)

This paper proposes a novel relaying-based approach for managing mobility in dense cellular networks. Dense deployment of base stations will have small cells, necessitating frequent handovers. Moreover, small cell solutions such as femtocells may lack low-delay backhaul connections to the operator core's network where handover procedures are coordinated. As a result, handover delays can be large, impacting signal quality severely. The proposed relay-based hierarchical mobility scheme forwards data over-the-air to other base stations close to mobile as the mobile moves but before the network point of attachment can be switched. Hybrid ARQ and randomized beamforming are used to opportunistically gain the benefits of relays with no explicit coordination between the relay base stations and the source or mobile. Thus, the protocol is attractive for high-speed dynamic environments and can be implemented with minimal messaging overhead. A simulation of the protocol in a 3GPP Long-Term Evolution (LTE) cellular system with dense cells and high-speed mobiles shows the ability of the protocol to mitigate short-term rate outages during handover with significant improvements for delay sensitive applications.
Effect-Based Approach to Assess Nanostructured Cellulose Sponge Removal Efficacy of Zinc Ions from Seawater to Prevent Ecological Risks (2020)

To encourage the applicability of nano-adsorbent materials for heavy metal ion removal from seawater and limit any potential side effects for marine organisms, an ecotoxicological evaluation based on a biological effect-based approach is presented. ZnCl<sub>2</sub> (10 mg L<sup>-1</sup>) contaminated artificial seawater (ASW) was treated with newly developed eco-friendly cellulose-based nanosponges (CNS) (1.25 g L<sup>-1</sup> for 2 h), and the cellular and tissue responses of marine mussel <i>Mytilus galloprovincialis</i> were measured before and after CNS treatment. A control group (ASW only) and a negative control group (CNS in ASW) were also tested. Methods: A significant recovery of Zn-induced damages in circulating immune and gill cells and mantle edges was observed in mussels exposed after CNS treatment. Genetic and chromosomal damages reversed to control levels in mussels' gill cells (DNA integrity level, nuclear abnormalities and apoptotic cells) and hemocytes (micronuclei), in which a recovery of lysosomal membrane stability (LMS) was also observed. Damage to syphons, loss of cilia by mantle edge epithelial cells and an increase in mucous cells in ZnCl<sub>2</sub>-exposed mussels were absent in specimens after CNS treatment, in which the mantle histology resembled that of the controls. No effects were observed in mussels exposed to CNS alone. As further proof of CNS' ability to remove Zn(II) from ASW, a significant reduction of >90% of Zn levels in ASW after CNS treatment was observed (from 6.006 to 0.510 mg L<sup>-1</sup>). Ecotoxicological evaluation confirmed the ability of CNS to remove Zn from ASW by showing a full recovery of Zn-induced toxicological responses to the levels of mussels exposed to ASW only (controls). An effect-based approach was thus proven to be useful in order to further support the environmentally safe (ecosafety) application of CNS for heavy metal removal from seawater.
Detection of candidal antigens in autoimmune polyglandular syndrome type I (1996)

Autoimmune polyglandular syndrome type I (APS I) is associated with chronic mucocutaneous candidiasis. To characterize the antibody responses in this subgroup of Candida albicans infections, we screened a candidal cDNA expression library with patient sera and found four cDNA clones encoding the immunopositive proteins enolase, heat shock protein 90, pyruvate kinase, and alcohol dehydrogenase. The reactivity to these antigens was studied further by immunoprecipitation assays with in vitro-transcribed and -translated proteins. Analysis of sera from 44 APS I patients showed that the highest antibody reactivity was found with enolase (80% of patients reactive), but significant serological responses were also found with heat shock protein 90 (67%), pyruvate kinase (62.5%), and alcohol dehydrogenase (64%). Overall, 95.5% of patients had detectable antibodies to at least one of these proteins. The cDNAs of enolase and heat shock protein 90 were also expressed in Escherichia coli and studied by immunoblotting. Again, 84% of sera reacted with enolase, whereas 44% of sera reacted with heat shock protein 90. A good correlation between the two methods was found for both enolase (r = 0.86; n = 58; P < 0.001) and heat shock protein 90 (r = 0.71; n = 56; P < 0.001). Our results indicate that the four abundant candidal proteins are the major antigens and can be used as accurate markers of candidiasis in APS I patients. The immunoprecipitation assay described here is particularly useful for the rapid analysis of a large number of samples.
RF propagation in an HVAC duct system: impulse response characteristics of the channel (2003)

Usually, the heating, ventilation, and air conditioning (HVAC) duct system in buildings is a complex network of hollow metal pipes of rectangular or circular cross-section which behave as multimode waveguides when driven at RF and microwave frequencies. The impulse response in the HVAC duct system used as a wireless communication channel is shaped by three physical mechanisms: probe coupling, attenuation, and dispersion. Three types of dispersion exist in this channel that affect the RMS delay spread. In order of importance, they are multipath reflections, intermodal dispersion, and intramodal dispersion. We present a model for the power delay profile in a straight terminated duct which allows exploration of RMS delay spread parametric behavior. As an example, we calculate the delay spread as a function of distance in a straight duct and find it to be in good agreement with experimental data at distances up to 15 m. A real HVAC system has a complicated geometry, which may include bends, junctions, etc. Efficient modelling of its channel properties is a challenging task. Our model for straight ducts should be perceived as a first step towards that goal.
Half-Life Extension Enhances Drug Efficacy in Adeno-Associated Virus Delivered Gene Therapy (2022)

Prolonged half-life of protein-based therapeutics can improve drug efficacy. However, the impact of drug half-life on gene therapy, which inherently provides long-lasting production of the desired therapeutic protein, remains unclear. In this study, several proteins with extended half-lives were engineered by fusion with the soluble monomeric immunoglobulin G 1 (IgG1) fragment crystallizable (sFc) or Fc region of IgG in adeno-associated virus (AAV)-delivered gene therapy. It was demonstrated that extending the half-life of a small-sized bifunctional protein and fibroblast growth factor 21 (FGF21) significantly increased their concentrations in the bloodstream circulation. Moreover, the half-life extension of AAV-delivered FGF21 resulted in a remarkable reduction in liver injury and blood glucose, and improved glucose tolerance and insulin sensitivity in type 2 diabetes mellitus animal models. These results demonstrate the therapeutic potential of gene therapy with prolonged drug half-life in the treatment of human diseases.
Anti-TRBC1 Antibody-Based Flow Cytometric Detection of T-Cell Clonality: Standardization of Sample Preparation and Diagnostic Implementation (2021)

A single antibody (anti-TRBC1; JOVI-1 antibody clone) against one of the two mutually exclusive T-cell receptor β-chain constant domains was identified as a potentially useful flow-cytometry (FCM) marker to assess Tαβ-cell clonality. We optimized the TRBC1-FCM approach for detecting clonal Tαβ-cells and validated the method in 211 normal, reactive and pathological samples. TRBC1 labeling significantly improved in the presence of CD3. Purified TRBC1<sup>+</sup> and TRBC1<sup>-</sup> monoclonal and polyclonal Tαβ-cells rearranged TRBJ1 in 44/47 (94%) and TRBJ1+TRBJ2 in 48 of 48 (100%) populations, respectively, which confirmed the high specificity of this assay. Additionally, TRBC1<sup>+</sup>/TRBC1<sup>-</sup> ratios within different Tαβ-cell subsets are provided as reference for polyclonal cells, among which a bimodal pattern of TRBC1-expression profile was found for all TCRVβ families, whereas highly-variable TRBC1<sup>+</sup>/TRBC1<sup>-</sup> ratios were observed in more mature vs. naïve Tαβ-cell subsets (vs. total T-cells). In 112/117 (96%) samples containing clonal Tαβ-cells in which the approach was validated, monotypic expression of TRBC1 was confirmed. Dilutional experiments showed a level of detection for detecting clonal Tαβ-cells of ≤10<sup>-4</sup> in seven out of eight pathological samples. These results support implementation of the optimized TRBC1-FCM approach as a fast, specific and accurate method for assessing T-cell clonality in diagnostic-FCM panels, and for minimal (residual) disease detection in mature Tαβ<sup>+</sup> leukemia/lymphoma patients.
ras activation in experimental carcinogenesis. (1992)

We review experimental models of carcinogenesis in which the role of ras activation has been most thoroughly studied: skin, thymus, mammary gland and liver. Qualitative changes (point mutations), as well as quantitative changes (over-expression, increased gene dosage) contribute to the transforming phenotype induced by ras genes. The activation of the three different ras family members is associated with particular tumor types, carcinogenic agents, and carcinogenic stages, suggesting the ras proteins may be involved in different biological functions. Depending on the system, ras activation has been shown to be an early and/or a late event in the multi-step process of carcinogenesis. These data underscore the possible relationship between ras activation and cell type specificity, proliferation, differentiation or cell-cell interaction.
Cytotoxic properties of the anthraquinone derivatives isolated from the roots of Rubia philippinensis (2018)

Our results suggested that the compounds 4 (xanthopurpurin) and 5 (lucidin-ω-methyl ether) showed high selective toxicity towards breast cancer cells at lower concentrations without showing toxicity towards normal cells, thus could be of potential as new lead molecules in cancer treatment.
Biotransformation and Bioavailability of Tea Polyphenols: Implications for Cancer Prevention Research (2005)

Consumption of tea (Camellia sinensis) has been suggested to prevent cancer, heart disease, and other diseases. Animal studies have shown that tea and tea constituents inhibit carcinogenesis at a number of organ sites including the skin, lung, oral cavity, esophagus, stomach, liver, intestine, colon, and prostate. A number of potential cancer prevention mechanisms for the tea polyphenols have been proposed based mainly on studies with human cancer cells. These include protection from oxidative stress, induction of oxidative stress, inhibition of enzymes (MAP kinases, cyclin-dependent kinases, telomerase, etc.), and inhibition of growth factor-related cell signaling (epidermal growth factor and others). Whereas some studies report effects of epigallocatechin-3-gallate (EGCG) at submicromolar levels, most experiments require concentrations of greater than 10 or 20 μM to demonstrate the effect. In humans, mice, and rats, tea polyphenols undergo glucuronidation, sulfation, methylation, and ring fission. Recent reports also suggest that EGCG and other catechins may be substrates for active efflux. The peak plasma concentrations of EGCG, epigallocatechin (EGC), and epicatechin (EC) following oral administration of green tea are 0.04 -1 μM, 0.3-5 μM, and 0.1-2.5 μM, respectively. The plasma levels of theaflavins are much lower (~ 2 nM). The present chapter reviews the literature concerning the biotransformation and bioavailability of tea polyphenols. It is intended to serve as a guide for designing future bioavailability experiments and for interpreting mechanistic data regarding the actions of tea polyphenols in vitro and in vivo.
Contrast Measurements For Polymer Dispersed Liquid Crystal Displays (1988)

Electro-optical contrast ratio studies are made on polymer dispersed liquid crystal (PDLC) films with regard to automotive displays. Various methods of measuring and defining contrast ratio are compared and the dominating factors controlling each method are evaluated as related to PDLC displays. These methods are discussed in relation to the illumination technique used for display cells. Transmissive and reflective mode PDLC display test cells are measured with both black on white and white on black symbology. For evaluating direct view displays, contrast ratio measurements are made by using a TV/Video analyzer system. With transmission mode test cells, the contrast ratio is dependent upon the angle of illumination, and maximum values are observed with illumination angles in the range of 25-45° off normal, depending upon the PDLC film properties. Contrast ratio values from direct view lab models were in the 20-28 range for transmission mode displays and 10-26 for reflective displays. The use of colored filter segments in the reflective model resulted in similar contrast for all colors as for white. The lab models showed excellent viewability in bright ambient light, even direct sunlight, with increased brightness offsetting some decrease in contrast ratio.