gebhart/patrepeval huggingface dataset:
Decomposition of phosphonate esters adsorbed on aluminum oxide (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDecomposition of phosphonate esters adsorbed on aluminum oxideM. K. Templeton and W. Henry WeinbergCite this: J. Am. Chem. Soc. 1985, 107, 4, 774–779Publication Date (Print):February 1, 1985Publication History Published online1 May 2002Published inissue 1 February 1985https://pubs.acs.org/doi/10.1021/ja00290a006https://doi.org/10.1021/ja00290a006research-articleACS PublicationsRequest reuse permissionsArticle Views711Altmetric-Citations81LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Identification of QTLs Associated with Biological Nitrogen Fixation Traits in Soybean Using a Genotyping‐by‐Sequencing Approach (2018)

Although biological N fixation (BNF) is a key process for economic cultivation of soybean [ Glycine max (L.) Merr.], the trait is often neglected in breeding programs, mainly due to difficulties in evaluating nodulation and N fixation parameters. We used a genotyping‐by‐sequencing approach to provide a dense genome‐wide marker coverage with 1448 single nucleotide polymorphisms (SNPs) distributed broadly across the chromosomes of a soybean population. The mapping population was composted of 113 F 7:8 recombinant inbred lines, obtained by single‐seed descent method, derived from crossing of soybean cultivars ‘Bossier’ (high BNF capacity) and ‘Embrapa 20’ (medium BNF capacity). The traits evaluated were nodule number (NN), nodule dry weight (NDW), average NDW (ANDW; i.e., NDW/NN), and shoot dry weight (SDW). A genetic map was constructed with 1448 SNPs that generated 35 linkage groups totaling 1793 cM, thus covering ∼72% of the genome based on the consensus linkage map. The estimated heritability for NN, NDW, ANDW, and SDW was 0.41, 0.30, 0.30, and 49.11, respectively. Inclusive composite interval mapping identified two significant quantitative trait loci (QTLs) for ANDW at 67 cM on chromosome 13, with a logarithm of odds (LOD) score of 4.66. The additive effect of this locus was −0.31 mg nodule −1 , and it explained 18.13% of the phenotypic variation. Another QTL was identified for SDW at 24 cM on chromosome 19 and exhibited an LOD score of 3.93. The additive effect was of 0.57 g plant −1 , and it accounted for 14.93% of the phenotypic variation. Additive genetic effects contribute to the final phenotype. In the present study, Embrapa 20 was the major contributor to ANDW, and Bossier was the major contributor to SDW. The latter was previously reported using simple sequence repeat markers.
Immunoelectron microscopic localization of three key steroidogenic enzymes (cytochrome P450(scc), 3 beta-hydroxysteroid dehydrogenase and cytochrome P450(c17)) in rat adrenal cortex and gonads (2001)

The biosynthesis of steroid hormones in endocrine steroid-secreting glands results from a series of successive steps involving both cytochrome P450 enzymes, which are mixed-function oxidases, and steroid dehydrogenases. So far, the subcellular distribution of steroidogenic enzymes has been mostly studied following subcellular fractionation, performed in placenta and adrenal cortex. In order to determine in situ the intracellular distribution of some steroidogenic enzymes, we have investigated the ultrastructural localization of the three key enzymes: P450 side chain cleavage (scc) which converts cholesterol to pregnenolone; 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) which catalyzes the conversion of 3 beta-hydroxy-5-ene steroids to 3-oxo-4-ene steroids (progesterone and androstenedione); and P450(c17) which is responsible for the transformation of C(21) into C(19) steroids (dehydroepiandrosterone and androstenedione). Immunogold labeling was used to localize the enzymes in rat adrenal cortex and gonads. The tissues were fixed in 1% glutaraldehyde and 3% paraformaldehyde and included in LR gold resin. In the adrenal cortex, both P450(scc) and 3 beta-HSD immunoreactivities were detected in the reticular, fascicular and glomerular zones. P450(scc) was exclusively found in large mitochondria. In contrast, 3 beta-HSD antigenic sites were mostly observed in the endoplasmic reticulum (ER) with some gold particles overlying crista and outer membranes of the mitochondria. P450(c17) could not be detected in adrenocortical cells. In the testis, the three enzymes were only found in Leydig cells. Immunolabeling for P450(scc) and 3 beta-HSD was restricted to mitochondria, while P450(c17) immunoreactivity was exclusively observed in ER. In the ovary, P450(scc) and 3 beta-HSD immunoreactivities were found in granulosa, theca interna and corpus luteum cells. The subcellular localization of the two enzymes was very similar to that observed in adrenocortical cells. P450(c17) could also be detected in theca interna cells of large developing and mature follicles. As observed in Leydig cells, P450(c17) immunolabeling could only be found in the ER. These results indicate that in different endocrine steroid-secreting cells P450(scc), 3 beta-HSD and P450(c17) have the same association with cytoplasmic organelles (with the exception of 3 beta-HSD in Leydig cells), suggesting similar intracellular pathways for biosynthesis of steroid hormones.
Rotating Disk Studies in Molten Carbonates: III . Diffusion Coefficient and Bulk Concentration in Lithium Carbonate (1994)

Steady‐state and transient measurements have been made at a rotating gold disk electrode in lithium carbonate at 800°C for simultaneously determining the diffusion coefficient and bulk concentration of peroxide ions. At high ratios where these measurements were made, the diffusion coefficient is found to be essentially independent of concentration with a mean value of . The bulk concentration of peroxide is two orders of magnitude higher than estimates based on thermodynamic calculations, but in agreement with values derived from oxygen solubility measurements in the literature.
Low-noise performance and dark-current measurements on the 256 x 256 NICMOS3 FPA (1993)

The NICMOS3 infrared focal plane array (FPA), which was designed as a Hubble Telescope upgrade device, provides excellent low-noise images in the 1 - 2.5 micrometers (SWIR) band. Both the detector array and the readout multiplexer of this hybrid FPA are optimized for low- noise operation. The NICMOS detector array is fabricated in HgCdTe grown on a sapphire substrate (PACE-I material). The sapphire substrate is very rugged and provides a good thermal contraction match to the silicon multiplexer, producing excellent reliability. The composition of the HgCdTe is adjusted to yield a response cutoff at 2.5 micrometers which limits the detector response to thermal background from the atmosphere and telescope. The quantum efficiency of the detectors is %GRT 50% over the 1 - 2.4 micrometers range. The dark current of the NICMOS detector is &lt; 1 e<SUP>MIN</SUP>/s at 77 K, which is unprecedented for an IR detector. The multiplexer is a switched-FET CMOS design with a single source-follower per unit cell. The photocurrent is integrated on each detector diode, and the diode voltage level can be read nondestructively, or reset after each readout. This flexibility in the FPA operation makes it possible to generate images at a 12 Hz data frame rate or to optimize for low-noise exposures of many thousands of seconds. With a readout before and after each reset, off-chip correlated double sampling can be implemented to reduce the read noise to &lt; 30 e<SUP>-</SUP>.
Propylene polymerization with Mg(OEt)2/benzoyl chloride/TiCl4–triethyl aluminum/external donor catalyst systems (1993)

Abstract Mg(OEt) 2 /benzoyl chloride (BC)/TiCl 4 catalyst has been prepared by the chemical reaction method, and the chemical composition and X‐ray structure of the catalyst were studied. The effect of various conditions on catalyst activity and isospecificity were also examined. With 13 C‐NMR analysis, it was found that aluminum carbinolate is formed during polymerization by reaction of triethyl aluminum cocatalyst with BC remaining in the prepared catalyst. The formed aluminum carbinolate acts as an additional donor to increase the isotactic index of polypropylene (PP). The morphology of the prepared catalyst and PP was also observed. © 1993 John Wiley &amp; Sons, Inc.
Large-gel two-dimensional electrophoresis-matrix assisted laser desorption/ionization-time of flight-mass spectrometry: An analytical challenge for studying complex protein mixtures (2001)

The large-gel two-dimensional electrophoresis (2-DE) technique, developed by Klose and co-workers over the past 25 years, provides the resolving power necessary to separate crude proteome extracts of higher eukaryotes. Matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) provides the sample throughput necessary to identify thousands of different protein species in an adequate time period. Spot excision, in situ proteolysis, and extraction of the cleavage products from the gel matrix, peptide purification and concentration as well as the mass spectrometric sample preparation are the crucial steps that interface the two analytical techniques. Today, these routines and not the mass spectrometric instrumentation determine how many protein digests can be analyzed per day per instrument. The present paper focuses on this analytical interface and reports on an integrated protocol and technology developed in our laboratory. Automated identification of proteins in sequence databases by mass spectrometric peptide mapping requires a powerful search engine that makes full use of the information contained in the experimental data, and scores the search results accordingly. This challenge is heading a second part of the paper.
The impact of EBV and HIV infection on the microenvironmental niche underlying Hodgkin lymphoma pathogenesis (2016)

The pathogenesis of classical Hodgkin lymphoma (cHL) is still enigmatic, largely because its tumor cells, the so-called Hodgkin and Reed-Stenberg (HRS) cells, invariably reside in a prominent reactive microenvironment, are rare and therefore difficult to analyze. On the other hand, the broadly investigated cHL-derived cell lines are not unequivocally considered as suitable and representative models for this puzzling disease. Based on current knowledge, it appears that the cross talk between the tumor cells and the reactive infiltrate of the microenvironment is complex and that multiple mechanisms occur, making cHL a very heterogeneous disease. In 20-40% of cHL cases, HRS cells carry a monoclonal infection by Epstein Barr virus (EBV), which is considered a tumor-initiating factor. In these cases, EBV shows a latency type II infection pattern with the expression of latent membrane protein-1 (LMP-1), a viral oncoprotein that mimics CD40 activation. This scenario is particularly intriguing for the pathogenesis of cHL arising in HIV-infected patients, which, for still obscure reasons, is invariably EBV-associated with LMP-1 expression in HRS cells. Recent evidences are consistent with the occurrence of different pathogenic pathways variably triggered by virus infections (EBV and HIV), genetic alterations, and interactions with critical microenvironmental components. This review focuses on the different microenvironmental niches that characterize cHL of the general population as well as cases of HIV-infected patients. A more comprehensive understanding of the complex interplay existing between HRS and tumor microenvironment is pivotal for the development of more effective treatments, particularly for relapsed or refractory diseases.
Pseudo‐Allosteric Recognition of Mandelic Acid with an Enantioselective Coordination Complex (2005)

Schwache Verknüpfungen wurden bei der Synthese eines vierfach koordinierten CuI-Komplexes zur chiralen Erkennung von Mandelsäure genutzt. Die Chelatbildung mit 2,2′-Bipyridin in einer Reaktion, bei der zugleich die schwachen Cu-S-Verknüpfungen gebrochen und die kondensierte Struktur zu einem 27-gliedrigen Makrocyclus geöffnet werden, wandelt den Komplex in einen enantioselektiven fluoreszierenden Rezeptor um. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2001/2006/z503343_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Decreased levels of osteopontin and bone sialoprotein II are correlated with reduced proliferation, colony formation, and migration of GFP-MDA-MB-231 cells (2004)

MDA-MB-231 human breast cancer cells transfected with GFP were used as model to determine the reduction in proliferation, colony formation, and migration in response to agents with anti-metastatic properties. These agents consisted of antisense oligonucleotides (ASOs) directed against osteopontin (OPN), bone sialoprotein II (BSP II), and osteonectin (ON), as well as an antibody directed against BSP II. A bisphosphonate derivative (ibandronate) and an alkylphosphocholine (erucylphospho-NNN-trimethylpropanolamine; ErPC3) were used as positive controls. The ASOs directed against OPN, BSP II and ON suppressed the expression of their respective target proteins by 81%, 74% and 69%, respectively. They were barely but significantly active in inhibiting the proliferation, but intermediately to highly active in inhibiting the colony formation and migration of GFP-MDA-MB-231 breast cancer cells. The antibody against human BSP II was significantly more active than all ASOs used and was equally active or even surpassed the activity of ibandronate and ErPC3 in all three assays. The results obtained suggest a specific anti-metastatic activity of this antibody as well as of the ASOs found effective in decreasing OPN and BSP II expression.
Integrated X-Ray, NMR, ECD, and Computational Approaches to Assign the Stereochemistry of Nortriterpenoids (2019)

Highly oxygenated nortriterpenoids are the principle bioactive chemical constituents isolated from plants of the genera Schisandra and Kadsura. The stereochemistry of these molecules remains a highly challenging problem to solve. To establish the stereochemistry and develop a general nuclear magnetic resonance-electric circular dichroism-computational approach to solve these complex metabolites, we reviewed the published methods to solve the stereochemistry of these triterpenoids. A key goal of this review is to provide a protocol to solve the problems hampering the assignment of the relative absolute configurations of other members of this natural product class.
OCean Observatories Initiative Scientific Data Model (2011)

The Ocean Observatories Initiative (OOI) through its Cyberinfrastructure (CI) Implementing Organization is developing a next generation platform for ocean sciences that will integrate a wide variety of information resources at scales unattainable before in the earth and ocean sciences. We introduce a novel scientific data model that enables distributed, large-scale storage and query of science data. Our model is built on multiple levels of abstraction ranging from domain-specific at the top down to encodings for message-oriented transport and persistence at the base. The key is exposing the properties of scientific feature types separately from the underlying structure of the data, which in turn is separated from their representation. The data representation is further isolated from the serialization and encoding used for transport and persistence. Our model greatly simplifies expressions of provenance and versioning of various data entities. It is robust, scalable and reliable. We implemented it for the first release of the OOI Integrated Observatory Network (ION), with rollout to operations currently underway.
System limitations due to chromatic dispersion and receiver bandwidth for 2-D time-wavelength OCDMA systems (2004)

We investigate chromatic-dispersion effects of pulse-spreading and wavelength-skew on a 2-D time-wavelength, optical code division multiple access system (OCDMA). We find that wavelength skew, which is specific to a bit-parallel system, overwhelmingly dominates the OCDMA system performance. For a 3-wavelength and 7-chip-time optimized code, a 10-Gchip/sec system is limited to under 4 kms.
Cyclic, Conformationally Constrained Melanotropin Analogs: Structure-Function and Conformational Relationships (2018)

This chapter reviews the structure-activity relationships in cyclic melanotropins and their inferences for further rational design of melanotropins, the structure-activity relationships of relevant selected linear melanotropins. The designation melanotropin has been applied to a large number of structurally similar peptides. A careful examination of the structure-activity relationships in these linear analogs and application of rational design techniques led to the preparation of a series of cyclic analogs of α-melanophore-stimulating hormone (α-MSH), many of which possess greater biological potency than their linear counterparts in the classical in vitro frog skin bioassay. Earlier structure-function studies of α-MSH have shown that the stereochemistry of the amino acid in the seven position of α-MSH has dramatic effects on the potency of the peptide. The in vitro biological assays allow quantitative measurements of the potency of melanotropins over a large dosage range. The contribution of the N-terminal tripeptide in α-MSH to the overall potency of the molecule has been examined extensively in linear analogs.
Fluorescence fluctuations-based super-resolution microscopy techniques: an experimental comparative study (2020)

Fluorescence fluctuations-based super-resolution microscopy (FF-SRM) is an emerging field promising low-cost and live-cell compatible imaging beyond the resolution of conventional optical microscopy. A comprehensive overview on how the nature of fluctuations, label density, out-of-focus light, sub-cellular dynamics, and the sample itself influence the reconstruction in FF-SRM is crucial to design appropriate biological experiments. We have experimentally compared several of the recently developed FF-SRM techniques (namely ESI, bSOFI, SRRF, SACD, MUSICAL and HAWK) on widefield fluorescence image sequences of a diverse set of samples (namely liposomes, tissues, fixed and living cells), and on three-dimensional simulated data where the ground truth is available. The simulated microscopy data showed that the different techniques have different requirements for signal fluctuation to achieve their optimal performance. While different levels of signal fluctuations had little effect on the SRRF, ESI and SACD images, image reconstructions from both bSOFI and MUSICAL displayed a substantial improvement in their noise rejection, z-sectioning, and overall super-resolution capabilities.
S113 The influenza virus activates TGFss via an Vss6-integrin mediated pathway (2011)

<h3>Introduction and Objectives</h3> Idiopathic pulmonary fibrosis is a chronic progressive lung disease of unknown cause. Its pathogenesis is poorly understood but activation of latent TGFß on lung epithelium is an important factor. TGFß must be activated, as it is secreted in a latent complex with its propeptide, the latency associated peptide, and the avß6 integrin is a key activator in the lung. The <i>Influenza A</i> virus is a single-stranded segmented RNA virus that infects epithelial cells leading to cell death and injury. Toll-like receptors (TLRs) detect pathogens, such as influenza. TLR3 activation has been found to increase RhoA activity. We previously showed that RhoA is a key intermediary in avß6 integrin-mediated TGFß activation. The aim of this study is to investigate whether influenza can activate TGFß and stimulate TLR3 leading to activation of TGFß through the avß6 integrin in epithelial cells. <h3>Materials and Methods</h3> Immortalised human bronchial epithelial cells (iHBECs) were infected with influenza A (H2N3) virus at a multiplicity of infection 1 with, or without, the avß6 blocking antibody 6.3G9. iHBECs were also stimulated with the synthetic TLR3 ligand poly(I:C). TGFß activity was determined by: (1) immunoblotting for phosphorylated (phospho-) Smad2, and (2) Transformed mink lung cells (TMLC)-iHBEC cocultures. Infection efficiency was measured by Interferon ß mRNA levels by real-time qPCR. <h3>Results</h3> Infection with H2N3 and stimulation with poly(I:C) led to increase in phospho-smad2 and luciferase activity in coculture indicating increase in TGFß activation levels in a dose- and time-dependent manner. In both cases this was blocked with the addition of 6.3G9. qPCR data following infection showed increased IFNß1 and PAI-1, indicating the ability of the virus to infect the cells and activate TGFß. <h3>Conclusions</h3> Influenza infection and poly(I:C) activates TGFß in iHBECs in an avß6 integrin dependent manner. The data suggests a novel mechanism by which influenza infection of epithelial cells may promote lung fibrosis.
Photoelectron Spectra of Molecules. I. Ionization Potentials of Some Organic Molecules and Their Interpretation (1969)

The photoelectron spectra of 67 organic compounds, including paraffins, cycloparaffins, olefines, dienes, aromatic hydrocarbons, alcohols, ethers, aldehydes, ketones, and diene iron tricarbonyl complexes, have been measured, using a simple retarding-potential grid-type spectrometer, and the results interpreted in terms of a recently developed semiempirical SCF MO treatment (MINDO approximation). The observed ionization potentials show a remarkably close relationship to the calculated Hartree–Fock MO energies over the whole range of ionization potentials (&amp;lt;20 eV) studied. An estimate is given for the first ionization potential of cyclobutadiene.
Ieee 802.11ad (2010)

Multi-Gbps communication is the next frontier in high-speed local and personal wireless technologies, which will offer the necessary foundation for a new wave of applications such as wireless display, high-speed device synchronization, and the evolution of Wi-Fi. The wide harmonized spectrum in the unlicensed millimeter-wave (60 GHz) band is considered the most prominent candidate to support the evolution towards multi-Gbps data rates. As such, the industry is in the process of defining new 60 GHz PHY and MAC technologies that can serve a wide variety of applications and usages, as to avoid the proliferation of non-coexistent devices operating in this unoccupied spectrum. The most promising activity is taking place under the auspices of the IEEE 802.11ad task group, which is defining amendments to the 802.11 standard for operation in the 60GHz band. In this paper we describe the main components of the MAC and PHY amendments included in the current IEEE 802.11ad draft standard and that enable multi-Gbps data rates. We also provide a comprehensive set of simulation results for typical use cases, and argue that 802.11ad is poised to be the standard that will enable mass market adoption of multi-Gbps wireless communication in the 60 GHz spectrum band.