gebhart/patrepeval huggingface dataset:
A pH-Triggered, Self-Assembled, and Bioprintable Hybrid Hydrogel Scaffold for Mesenchymal Stem Cell Based Bone Tissue Engineering (2019)

Effective bone tissue engineering can restore bone and skeletal functions that are impaired by traumas and/or certain medical conditions. Bone is a complex tissue and functions through orchestrated interactions between cells, biomechanical forces, and biofactors. To identify ideal scaffold materials for effective mesenchymal stem cell (MSC)-based bone tissue regeneration, here we develop and characterize a composite nanoparticle hydrogel by combining carboxymethyl chitosan (CMCh) and amorphous calcium phosphate (ACP) (designated as CMCh-ACP hydrogel). We demonstrate that the CMCh-ACP hydrogel is readily prepared by incorporating glucono δ-lactone (GDL) into an aqueous dispersion or rehydrating the acidic freeze-dried nanoparticles in a pH-triggered controlled-assembly fashion. The CMCh-ACP hydrogel exhibits excellent biocompatibility and effectively supports MSC proliferation and cell adhesion. Moreover, while augmenting BMP9-induced osteogenic differentiation, the CMCh-ACP hydrogel itself is osteoinductive and induces the expression of osteoblastic regulators and bone markers in MSCs in vitro. The CMCh-ACP scaffold markedly enhances the efficiency and maturity of BMP9-induced bone formation in vivo, while suppressing bone resorption occurred in long-term ectopic osteogenesis. Thus, these results suggest that the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel may be further exploited as a novel scaffold for osteoprogenitor-cell-based bone tissue regeneration.
Ultralow Power Silicon Modulators (2014)

We discuss recent results in low-energy (0.9fJ/bit) high-speed (25Gb/s) electro-optically stabilized microdisk modulators. The combined results of highly efficient modulation and electro-optic thermal compensation represent a major step towards single-digit femtojoule-class communications.
The Influence of Nitric Oxide on the Occurrence of Autoignition in the End Gas of Spark Ignition Engines (2002)

&lt;div class="htmlview paragraph"&gt;Full cycle simulations of a spark ignition engine running on a primary reference fuel have been performed using a two-zone model. A detailed kinetic mechanism is taken into account in each of the zones, while the propagating flame front is calculated from a Wiebe function. The initial conditions for the unburned gas zone were calculated as a mixture of fresh gas and rest gas. The composition of the burned gas zone at the end of the last engine cycle, including nitric oxide emissions, was taken as rest gas.&lt;/div&gt; &lt;div class="htmlview paragraph"&gt;The simulations confirm that the occurrence of autoignition in the end gas is sensitive on the amount of nitric oxide in the rest gas of the spark ignition engine. The comparison of autoignition timings calculated for a single cylinder test engine are getting more accurate if the nitric oxide in the initial gases is taken into account.&lt;/div&gt;
IFN-γ in Combination with IL-3 Accelerates Platelet Recovery in Mice with 5-Fluorouracil-Induced Marrow Aplasia (1996)

The effects of interferon-gamma (IFN-gamma) on platelet recovery were examined in mice with marrow aplasia induced by i.p. injection of 250 mg/kg of 5-fluorouracil (5-FU). The cytokine was administrated by microosmotic pump, with an ability to deliver a consistent intact dose of cytokine for 7 consecutive days. Administration of 250 IU/kg/day of IFN-gamma in combination with 10(3) U/kg/day of IL-3, which alone had no effect on platelet counts, diminished the nadir for platelet count and shortened the duration of thrombocytopenia. The effect was comparable to that of higher doses of IL-3 (10(5) U/kg/day). The administration of 250 IU/kg/day of IFN-gamma in combination with 10(3) U/kg/day of IL-3 also induced megakaryocyte proliferation in bone marrow cell cultures. Single administration of either 250 IU/kg/day of IFN-gamma or 10(3) U/kg/day of IL-3 had no significant effects. The effect of this combination was also comparable to that of a higher dose of IL-3 (10(5) U/kg/day). We suggest that IFN-gamma accelerates megakaryocyte development, which leads to platelet production in chemotherapy-induced marrow aplasia. The administration of IFN-gamma in combination with IL-3 might be useful for the management of marrow aplasia.
Abstract 1154: DCLK1 monoclonal antibody-based CAR-T cells as novel treatment strategy against multiple myeloma (2019)

Abstract Multiple Myeloma (MM) is characterized by the clonal expansion of malignant plasma cells in the bone marrow, and is the second most common hematological cancer. Cellular therapies including chimeric antigen T receptor cell (CAR-T) have emerged as a promising therapeutic strategy to combat this devastating disease. MM are derived from a rare population of cancer stem cells (CSC) that are responsible for initiation, progression, resistance to conventional therapy and recurrence. To date there have been no CAR-T therapies directed at CSC-specific antigens. In this report, we describe the effects of a novel CAR-T targeting the CSC marker DCLK1 on MM cells. Using mouse models, it has been demonstrated that DCLK1 marks the cell of origin of colon and pancreatic cancers. DCLK1 regulates epithelial to mesenchymal transition (EMT), and its upregulation is associated with poor overall survival in many cancer subtypes. Inhibition of DCLK1 activity following RNA silencing resulted in tumor xenograft growth arrest. These features lead us to explore the therapeutic potential of targeting DCLK1+ cells in MM. In order to create a novel DCLK1 CAR, we generated a new humanized DCLK1 monoclonal antibody, clone CBT-15 #8. This antibody recognizes a small extra cellular C-terminal epitope on human isoform 2 and 4 of DCLK1. Novel DCLK1 CAR-T cells containing the clone were generated and detected with anti-human FAB antibody by flow cytometry. Furthermore, we observed cell surface expression of DCLK1 on &amp;gt;93 percent of MM Cells (RPMI8226) by flow cytometry. The CAR-T cells had significantly higher cytotoxicity in MM (RPMI8226 and MM1S) cells than in leukemia K562 cells. The CAR-T cells secreted significantly higher levels of IFN-γ in vitro than T cells (p=0.0036). Further studies are underway to determine its efficacy in inhibiting tumor xenografts in vivo. This report is the first to link the TSC marker DCLK1 to MM and may represent a novel therapeutic strategy for MM and other hematologic malignancies. Citation Format: Sripathi M. Sureban, Robert Berahovich, Hua Zhou, Shirley Xu, Lijun Wu, Vita Golubovskaya, Dongfeng Qu, Randal May, Edwin Bannerman-Menson, Courtney W. Houchen. DCLK1 monoclonal antibody-based CAR-T cells as novel treatment strategy against multiple myeloma [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 1154.
Organometallic chemistry. 22. Triphenylsilyl perchlorate revisited: silicon-29 and chlorine-35 NMR spectroscopy and x-ray crystallography showing covalent nature in both solution and the solid state. Difficulties in observing long-lived silyl cations in the condensed state (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOrganometallic chemistry. 22. Triphenylsilyl perchlorate revisited: silicon-29 and chlorine-35 NMR spectroscopy and x-ray crystallography showing covalent nature in both solution and the solid state. Difficulties in observing long-lived silyl cations in the condensed stateG. K. Surya Prakash, Schahab Keyaniyan, Robert Aniszfeld, Ludger Heiliger, George A. Olah, Raymond C. Stevens, Hok Kin Choi, and Robert BauCite this: J. Am. Chem. Soc. 1987, 109, 17, 5123–5126Publication Date (Print):August 1, 1987Publication History Published online1 May 2002Published inissue 1 August 1987https://pubs.acs.org/doi/10.1021/ja00251a013https://doi.org/10.1021/ja00251a013research-articleACS PublicationsRequest reuse permissionsArticle Views307Altmetric-Citations73LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Enhanced Nonlinear Optics Using an Atomic Local Oscillator (1996)

Nonlinear optical mixing of existing laser frequencies to access portions of the spectrum where lasing action is not easily obtainable is common practice today. Although nonlinear solid-state media are available [1] for frequency conversion from just under 200 nm in the ultraviolet to about a few microns in the infrared, the generation of widely-tunable laser radiation in the mid-to-far infrared and VUV regions typically requires four-frequency mixing in atomic vapors. In the past, frequency conversion in atomic vapors suffered from poor conversion efficiencies due to the small magnitude of the third-order nonlinear susceptibility and the difficulty in phasematching. We demonstrate a method [2] for performing four-frequency mixing which overcomes both of these shortcomings, allowing efficient frequency conversion with moderate pump intensities.
Overexpression of GILZ in macrophages limits systemic inflammation while increasing bacterial clearance in sepsis in mice (2019)

Studies support the beneficial effects of glucocorticoids (GCs) during septic shock, steering research toward the potential role of GC-induced proteins in controlling excessive inflammatory responses. GILZ is a glucocorticoid-induced protein involved in the anti-inflammatory effects of GCs. We investigated whether the overexpression of GILZ specifically limited to monocytes and macrophages (M/M) alone could control inflammation, thus improving the outcome of septic shock in animal models. We also monitored the expression of GILZ in M/M from septic mice and septic-shock patients. M/M from patients and septic mice displayed significantly lower expression of GILZ than those isolated from controls. Furthermore, transgenic mice (Tg-mice) experiencing sepsis, with increased expression of GILZ restricted to M/M, showed lower frequencies of inflammatory monocytes than their littermates and lower plasma levels of inflammatory cytokines. Tg-mice also had lower blood bacterial counts. We further established that the upregulation of GILZ in M/M enhanced their phagocytic capacity in in vivo assays. The increase of GILZ in M/M was also sufficient to improve the survival rates of septic mice. These results provide evidence for a central role of both GILZ and M/M in the pathophysiology of septic shock and a possible clue for the modulation of inflammation in this disease.
Spectacle Lenses With Aspherical Lenslets for Myopia Control vs Single-Vision Spectacle Lenses (2022)

<h3>Importance</h3> Reducing myopia progression can reduce the risk of associated ocular pathologies. <h3>Objective</h3> To evaluate whether spectacle lenses with higher lenslet asphericity have a higher myopia control efficacy throughout 2 years. <h3>Design, Setting, and Participants</h3> This double-masked randomized clinical trial was conducted between July 2018 and October 2020 at the Eye Hospital of Wenzhou Medical University in Wenzhou, China. Children aged 8 to 13 years with a cycloplegic spherical equivalent refraction (SER) of −0.75 D to −4.75 D and astigmatism with less than −1.50 D were recruited. A data and safety monitoring committee reviewed findings from a planned interim analysis in 2019. <h3>Interventions</h3> Participants were randomly assigned in a 1:1:1 ratio to receive spectacle lenses with highly aspherical lenslets (HAL), spectacle lenses with slightly aspherical lenslets (SAL), or single-vision spectacle lenses (SVL). <h3>Main Outcome and Measures</h3> Two-year changes in SER and axial length and their differences between groups. <h3>Results</h3> Of 157 participants who completed each visit (mean [SD] age, 10.4 [1.2] years), 54 were analyzed in the HAL group, 53 in the SAL group, and 50 in the SVL group. Mean (SE) 2-year myopia progression in the SVL group was 1.46 (0.09) D. Compared with SVL, the mean (SE) change in SER was less for HAL (by 0.80 [0.11] D) and SAL (by 0.42 [0.11] D;<i>P</i> ≤ .001). The mean (SE) increase in axial length was 0.69 (0.04) mm for SVL. Compared with SVL, increase in axial length was slowed by a mean (SE) of 0.35 (0.05) mm for HAL and 0.18 (0.05) mm for SAL (<i>P</i> ≤ .001). Compared with SVL, for children who wore HAL at least 12 hours every day, the mean (SE) change in SER was slowed by 0.99 (0.12) D, and increase in axial length slowed by 0.41 (0.05) mm. <h3>Conclusions and Relevance</h3> In this study, HAL and SAL reduced the rate of myopia progression and axial elongation throughout 2 years, with higher efficacy for HAL. Longer wearing hours resulted in better myopia control efficacy for HAL. <h3>Trial Registration</h3> Chinese Clinical Trial Registry Identifier:ChiCTR1800017683
Molecular imaging reporting and data systems (MI-RADS): a generalizable framework for targeted radiotracers with theranostic implications (2018)

Both prostate-specific membrane antigen (PSMA)- and somatostatin receptor (SSTR)-targeted positron emission tomography (PET)-based imaging agents for prostate carcinoma and neuroendocrine tumors, respectively, are seeing rapidly expanding use. In addition to diagnostic applications, both classes of radiotracers can be used to triage patients for theranostic endoradiotherapy. While interpreting PSMA- or SSTR-targeted PET/computed tomography (CT) scans, the reader has to be aware of certain pitfalls. Adding to the complexity of the interpretation of those imaging agents, both normal biodistribution, and also false-positive and -negative findings differ between PSMA- and SSTR-targeted PET radiotracers. Herein summarized under the umbrella term molecular imaging reporting and data systems (MI-RADS), two novel RADS classifications for PSMA- and SSTR-targeted PET imaging are described (PSMA- and SSTR-RADS). Notably, PSMA- and SSTR-RADS are structured in a reciprocal fashion, i.e., if the reader is familiar with one system, the other system can readily be applied, as well. In the present review, we will discuss the most common pitfalls on PSMA- and SSTR-targeted PET/CT, briefly introduce PSMA- and SSTR-RADS, and define a potential future role of the umbrella framework MI-RADS compared to other classification systems.
Preparation of Photoluminescent Porous Silicon Nanoparticles by High‐Pressure Microfluidization (2017)

The use of high-shear microfluidization as a rapid, reproducible, and high-yield method to prepare nanoparticles of porous silicon (pSi) with a narrow size distribution is described. Porous films prepared by electrochemical etch of a single-crystal silicon wafer are removed from the substrate, fragmented, dispersed in an aqueous solution, and then processed with a microfluidizer, which generates high yields (57%) of pSi nanoparticles of narrow size distribution (PDI = 0.263) without a filtration step. Preparation of pSi nanoparticles via microfluidization improves yields (by 2.4-fold) and particle size uniformity (by 1.8-fold), and it lowers the total processing time (by 36-fold) over standard ultrasonication or ball milling methods. The average diameter of the nanoparticles can be adjusted over the range 150–350 nm by appropriate adjustment of processing steps. If the fluid carrier in the microfluidizer contains an oxidant for Si, the resulting pSi particles are prepared with a core–shell structure, in which an elemental Si core is encased in a silicon oxide shell. When an aqueous sodium tetraborate processing solution is used, microfluidization generates photoluminescent core–shell pSi particles with a quantum yield of 19% in a single step in less than 20 min.
Controlled spontaneous emission in room-temperature semiconductor microcavities (1992)

Data are presented demonstrating controlled spontaneous emission in room-temperature AlGaAs-GaAs Fabry–Perot microcavities, which utilize high contrast Bragg reflectors. The reflector materials are a CaF2/ZnSe combination. A GaAs quantum well contained in the microcavities is excited using a low power He-Ne laser, and the spontaneous emission characteristics are measured in terms of spectral characteristics and radiation patterns. The measured data are compared with calculations which predict controlled spontaneous emission in such structures. We find that the dominant effects on spontaneous emission in these thin layer structures are due to cavity controlled emission into allowed optical modes and stress induced dipole orientation in the GaAs quantum well.
Towards the production of anti-hydrogen beams (2013)

Recently, the production of low energy anti-hydrogen atoms in the cusp trap with the use of 150eV antiproton beams from the MUSASHI trap was reported. The purpose of producing low energy anti-hydrogen atoms in a cusped magnetic field is to extract a polarized anti-hydrogen beam to a field free region, so that the hyperfine structure of anti-hydrogen atoms can be measured. © 2013 American Institute of Physics.
ChemInform Abstract: ((CO)5Cr=Tl=Cr(CO)5)‐, the First Complex Containing Linear Twofold‐ Coordinated Thallium. (1994)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Searching WormBase for Information About Caenorhabditis elegans (2004)

Abstract WormBase is the major public biological database for the nematode Caenorhabditis elegans . It is meant to be useful to any biologist who wants to use C. elegans , whatever his or her specialty. WormBase contains information about the genomic sequence of C. elegans , its genes and their products, and its higher‐level traits such as gene expression patterns and neuronal connectivity. Also, WormBase contains sequence and gene structures of C. briggsae , a closely related worm. These data are interconnected, so that a search beginning with one object (such as a gene) can be directed to related objects of a different type (such as the DNA sequence of the gene, or the cells in which the gene is active). One can also do searches for complex data sets. The WormBase developers group actively invites suggestions for improvement from users. WormBase's source code and underlying database are freely available for local installation and improvement.
DNA/Fusogenic Lipid Nanocarrier Assembly: Millisecond Structural Dynamics (2013)

Structural changes occurring on a millisecond time scale during uptake of DNA by cationic lipid nanocarriers are monitored by time-resolved small-angle X-ray scattering (SAXS) coupled to a rapid-mixing stopped-flow technique. Nanoparticles (NPs) of nanochannel organization are formed by PEGylation, hydration, and dispersion of a lipid film of the fusogenic lipid monoolein in a mixture with positively charged (DOMA) and PEGylated (DOPE-PEG2000) amphiphiles and are characterized by the inner cubic structure of very large nanochannels favorable for DNA upload. Ultrafast structural dynamics of complexation and assembly of these cubosome particles with neurotrophic plasmid DNA (pDNA) is revealed thanks to the high brightness of the employed synchrotron X-ray beam. The rate constant of the pDNA/lipid NP complexation is estimated from dynamic roentgenograms recorded at 4 ms time resolution. pDNA upload into the vastly hydrated channels of the cubosome carriers leads to a fast nanoparticle-nanoparticle structural transition and lipoplex formation involving tightly packed pDNA.
Experimental system for self-optimization of LTE networks (2012)

The aim of this paper is to present an experimental platform developed for 3GPP Long Term Evolution (LTE) networks where the effectiveness of self-optimization algorithms can be tested in a realistic environment. Several Self-organizing networks (SON) use cases are implemented in realistic scenarios. The system is developed to provide self-optimization functionalities in the framework of Self organizing networks (SON). The experimental system shown is built on top of radio network simulators and commercial network management products. In this work different commercial network management products like Radio Network Optimizer, Radio Network Simulator and Operational Subsystem (OSS) Middleware are integrated to provide automatic and efficient SON solutions, thus reducing human effort on the one hand and improving network performance in terms of coverage, capacity and service quality on the other. The network performance impacts of remote electrical antenna tilt (RET) optimization and transmission power optimization could be shown with the tool. Average cell throughput and per physical resource block (PRB) throughput are shown for constant bit rate (CBR) service before and after optimization. Coverage improvements are seen in improvements in handover (HO) failure statistics. Robustness and convergence time of optimization algorithms are studied.
Performance Evaluation Approach for Multi-Tier Cloud Applications (2013)

Complex multi-tier applications deployed in cloud computing environments can experience rapid changes in their workloads. To ensure market readiness of such applications, adequate resources need to be provisioned so that the applications can meet the demands of specified workload levels and at the same time ensure that service level agreements are met. Multi-tier cloud applications can have complex deployment configurations with load balancers, web servers, application servers and database servers. Complex dependencies may exist between servers in various tiers. To support provisioning and capacity planning decisions, performance testing approaches with synthetic workloads are used. Accuracy of a performance testing approach is determined by how closely the generated synthetic workloads mimic the realistic workloads. Since multi-tier applications can have varied deployment configurations and characteristic workloads, there is a need for a generic performance testing methodology that allows accurately modeling the performance of applications. We propose a methodology for performance testing of complex multi-tier applications. The workloads of multi-tier cloud applications are captured in two different models-benchmark application and workload models. An architecture model captures the deployment configurations of multi-tier applications. We propose a rapid deployment prototyping methodology that can help in choosing the best and most cost effective deployments for multi-tier applications that meet the specified performance requirements. We also describe a system bottleneck detection approach based on experimental evaluation of multi-tier applications.