gebhart/patrepeval huggingface dataset:
Abstract 5490: The cytotoxic activity of the anti-mesothelin immunotoxin SS1P is enhanced by the PKC inhibitor enzastaurin. (2013)

Abstract The activation of protein kinase C (PKC) contributes to tumor survival and proliferation provoking the development of inhibitory agents that may function as cancer therapeutics. Pseudomonas exotoxin-based immunotoxins are antibody-based recombinant proteins that employ an ADP-ribosylation domain for the enzymatic inhibition of protein synthesis of target cancer cells. Combining PKC inhibitors with immunotoxins was undertaken to explore possible synergy. Enzastaurin but not two other PKC inhibitors (Sortastaurin and G06976) combined with immunotoxins directed to surface mesothelin or the transferrin receptor to produce synergistic or additive cell death via apoptosis. Enhancement of killing was noted with enzastaurin concentrations of 5 uM or greater. Mechanistic insights of the combined immunotoxin-enzastaurin killing centered on the rapid complete loss of the prosurvival Bcl2 protein, Mcl-1, and the activation of caspase 3/7. Moreover, efficient cell killing was associated with loss of Akt and the BH3-only protein Bad. Enzastaurin-mediated enhanced cell killing was specific to Pseudomonas exotoxin-based immunotoxins as there was no enhancement with other agents that inhibit protein synthesis such as cycloheximide and diphtheria toxin. Immunotoxin-enzastaurin combinations could be useful for the treatment of human cancers that express mesothelin and are not efficiently killed by SS1P alone. Citation Format: Abid R. Mattoo, Ira Pastan, David Fitzgerald. The cytotoxic activity of the anti-mesothelin immunotoxin SS1P is enhanced by the PKC inhibitor enzastaurin. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 5490. doi:10.1158/1538-7445.AM2013-5490
Influence of effective channel length in self-aligned coplanar amorphous-indium-gallium-zinc-oxide thin-film transistors with different annealing temperatures (2018)

Electrical characteristics of self-aligned coplanar amorphous-indium-gallium-zinc-oxide (a-IGZO) thin-film transistors (TFTs) were investigated for different annealing temperatures. The field-effect mobility of the a-IGZO TFTs increased with the annealing temperature, in particular, for a small channel length. The effective channel length (Leff) of the a-IGZO TFTs was extracted using the transmission line method. The decrease in Leff significantly depended on the annealing temperature, due to the hydrogen diffusion into the a-IGZO channel region. The intrinsic mobility calculated from the channel resistance of the a-IGZO TFTs was in good agreement with the mobility corrected by Leff.
3D surface mapping using a semi-autonomous rover: A planetary analog field experiment (2012)

This paper describes a proposed operational architecture for a planetary worksite mapping mission concept. To map three-dimensional (3D) planetary terrain, we pro-pose to use a rover equipped with a laser rangefinder, and employ a stop-scan-go approach with a human-in-the-loop. In the operational cycle, the rover collects locally consis-tent 3D range data while stationary. The range data are coupled with visual odometry to estimate the rover pose at each scan and create a consistent 3D map. The 3D map is then used to evaluate candidate next-best views (NBV). The operator selects a NBV with the aid of three evalu-ation criteria and the rover autonomously travels to the NBV using a network of reusable paths (NRP). Finally, the rover collects another 3D scan and the cycle repeats. This mission concept was validated through hardware ex-periments on the CSA’s Mars Emulation Terrain (MET), which measures 60m × 120m and includes inclines, rocks, cliffs and a 5.5m-diameter crater.
Non-Coding RNAs in Human Breast Milk: A Systematic Review (2021)

PROSPERO https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=138989, identifier CRD42020138989.
Strong Nonlinear Coupling in a Si3N4 Ring Resonator (2019)

We demonstrate that nondegenerate four-wave mixing in a Si_{3}N_{4} microring resonator can result in a nonlinear coupling rate between two optical fields exceeding their energy dissipation rate in the resonator, corresponding to strong nonlinear coupling. We demonstrate that this leads to a Rabi-like splitting, for which we provide a theoretical description in agreement with our experimental results. This yields new insight into the dynamics of nonlinear optical interactions in microresonators and access to novel phenomena.
Local Competition and Universal Service (1995)

Public policy in the telecommunications industry has largely been driven by two goals: economic efficiency and universal service. The possibility of conflict between the two goals arises as&#13;\nthe local telecommunications markets are opened up to competition and as&#13;\nthe information age at the same time is fundamentally changing the nature of&#13;\ntelecommunications. How then do we allow competition to discipline the&#13;\nmarket and ensure that society is not divided into the "information rich" and&#13;\nthe "information poor"? In this essay we outline some of the policy options.
Synthesis of 18F-labeled aromatic and heteroaromatic compounds using 1,3-dipolar-cycloaddition reactions with o-benzyne (2013)

617 Objectives (3+2) Dipolar cycloadditions of arynes with different dipoles (CAAs) are widely used in the syntheses of (hetero-)aromatic compounds and pharmaceuticals. Despite of their fast reaction kinetics and versatility CAAs have not yet been applied for radiolabeling. The goal was to evaluate the feasibility of CAAs for the preparation of radiofluorinated compounds. The reaction between o-benzyne and various 18F-labeled 1,3-dipoles was studied. Methods o-Benzyne was generated in situ by thermal decomposition of benzediazonium-2-carboxylate (thermal route) or by fluoride-promoted ortho-elimination of o-(trimethylsilyl)phenyl triflate (fluoride-promoted route) and allowed to react with the appropriate 18F-labeled 1,3-dipole. The following compounds were prepared: 3-(4-[18F]fluorophenyl)-benzisoxazole ([18F]1), 2-tert-butyl-3-(4-[18F]fluorophenyl)-1,2-benzisoxa-zoline ([18F]2), (4-[18F]fluorophenyl)-2-hydroxybenzophenone methylimine ([18F]3), 3-(4-[18F]fluorophenyl)indazole ([18F]4), 3-(4-[18F]fluorophenyl)-1-phenyl-indazole ([18F]5) and 1-(2-[18F]fluoroethyl)-benzotriazole ([18F]6). Results [18F]1-3 were prepared by the thermal route in RCY of 60-70% (MeCN, 10 min, 50-80 °C) from 4-[18F]fluorobenzonitriloxide, C-4-[18F]fluorophenyl-N-tert-butylnitrone and C-4-fluorophenyl-N-methylnitrone respectively. The fluoride-promoted route yielded [18F]1, [18F]4 and [18F]5 in 20-90% RCY (TBAF, THF, 10 min, 50 °C). [18F]4 and [18F]5 were prepared from the newly generated 1,3-dipoles, 4-[18F]fluorophenyl-diazomethane and 4-[18F]fluorobenzonitrile-N-phenylimine, respectively. [18F]6 was prepared from 2-[18F]fluoroethylazide in RCYs of 65% and 90% (fluoride-promoted and thermal route, respectively). Conclusions The CAA reactions with radiofluorinated 1,3-dipoles provide a new, efficient tool for an easy preparation of 18F-labeled homo/heterocyclic compounds hardly accessible via alternative radiolabeling methods. Research Support Supported by the EFRE-Ziel2 program (North-Rhine-Westphalia, Germany).
Metal-Alkyl Group Effects on the Thermodynamic Stability and Stereochemical Mobility of B(C6F5)3-Derived Zr and Hf Metallocenium Ion-Pairs (2000)

This paper reports significant dependence of ion-pair formation energetics and stereomutation rates upon the metal-bound alkyl substituent (R) and the solvent dielectric constant in the metallocenium series (1,2-Me2Cp)2MR+ MeB(C6F5)3- where R = Me, CH2tBu, CH2TMS, CH(TMS)2, and M = Zr and Hf, as determined by reaction titration calorimetry and dynamic NMR spectroscopy. For the ion-pair forming reaction, (1,2-Me2Cp)2M(R)Me + B(C6F5)3 → (1,2-Me2Cp)2MR+ MeB(C6F5)3-, enthalpies in toluene solution at 25 °C for M = Zr and R = Me, CH2TMS, and CH(TMS)2 are −24.6(0.8), −22.6(1.0), and −59.2(1.4) kcal/mol, respectively. Corresponding values for M = Hf and R = Me and CH2TMS are −20.8(0.5) and −31.1(1.6) kcal/mol, respectively. ΔH‡reorg values for the reorganization process that interchanges the ion-pair enantiotopic sites for M = Zr and R = Me, CH2tBu, CH2TMS, and CH(TMS)2 are 22(1), 18(1), 17(1), and 9(2) kcal/mol, respectively. Corresponding ΔH‡reorg values for M = Hf and R = Me, CH2tBu, and CH2TMS are >24, 12(3), and 15(2) kcal/mol, respectively. ΔH‡reorg values are highest in low dielectric constant solvents such as C6D12. Activation parameters for β-Me elimination in the complexes (1,2-Me2Cp)2MCH2tBu+ MeB(C6F5)3- for M = Zr and Hf are found to be ΔH‡β-Me = 22.5(0.9) and 17.3(0.9) kcal/mol, and ΔS‡β-Me = 4.3(3.3) and −11.9(3.4) cal/mol·K, respectively.
[(salen)Al]-Mediated, Controlled and Stereoselective Ring-Opening Polymerization of Lactide in Solution and without Solvent: Synthesis of Highly Isotactic Polylactide Stereocopolymers from Racemic d,l-Lactide (2002)

Easily accessible [(salen)(iPrO)Al] exerts excellent molecular-weight and stereochemical control in lactide polymerization either in solution or in the absence of solvent. The R,R initiator shows a marked preference for L-lactide over D-lactide. Stereoblock copolylactides (see scheme) with high melting points can be prepared directly from d,l-lactides by using a racemic initiator. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2002/z19632_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Magnetic and Transport Properties of Electrochemically Oxidized Polyacetylene (1985)

The magnetic and transport properties of polyacetylene electrochemically oxidized in the presence of perchlorate ion have been determined. Room temperature dc conductivity is unaffected by doping between 10 -6 < y -< 10 -3~. A rapid conductivity increase occurs with increasing dopant level having a midpoint of transition at ca. y - 8 x 10-3 By comparison, the change in thermopower vs. y is sharper taking place within a tenfold increase in y with a midpoint at ca. y - 3 x 10 -3. The unparied spin concentrations remain constant as dopant concentration increases before the onset of a Dysonian lineshape at ca. y -= 10 -2. The results are interpreted as the melting of a glassy carrier state causing the sharp change of transport properties. A phase separation into metallic domains occurs at y = ca. 10 -~. Side reactions involving the electrolyte complicate the electrochemistry of po]yacetylene.
Automated Fiber Optic Biosensor for Multiplexed Immunoassays (2000)

The multianalyte capability of the RAPTOR, a rapid, automatic, and portable fiber optic fluorimeter, was demonstrated. Employing evanescent wave illumination on polystyrene fiber optic waveguides, the RAPTOR performed fluorescent immunoassays for Bacillus globigii spores, ovalbumin, Erwinia herbicola, and MS2 coliphage. During a 4-day laboratory trial assaying 144 blind samples, the RAPTOR demonstrated detection of 105 cfu/mL B. globigii, 107 cfu/mL E. herbicola, and 109 pfu/mL MS2; ovalbumin detection was less favorable than expected due to sample degradation. Assays were completed in 10 min with no sample preprocessing. No false positives were identified. Antigen carryover between coupons was examined but was not found to elicit a notable response for any analytes, except B. globigii. Finally, assay results obtained after reagent and waveguides had completed 30 negative (buffer) cycles were compared with standard assay results achieved with fresh reagent and waveguides to determine whether antigen detection would decrease using cycled reagent or optical probes. Detection efficacy proved to be unaffected by the use of cycled versus fresh probes or reagent.
A lymphoma plasma membrane-associated protein with ankyrin-like properties. (1986)

In this study we have used several complementary techniques to isolate and characterize a 72-kD polypeptide that is tightly associated with a major mouse T-lymphoma membrane glycoprotein, gp 85 (a wheat germ agglutinin-binding protein), in a 16 S complex. These two proteins do not separate in the presence of high salt but can be dissociated by treatment with 2 M urea. Further analysis indicates that the 72-kD protein has ankyrin-like properties based on the following criteria: (a) it cross-reacts with specific antibodies raised against erythrocyte and brain ankyrin; (b) it displays a peptide mapping pattern and a pI (between 6.5 and 6.8) similar to that of the 72-kD proteolytic fragment of erythrocyte ankyrin; (c) it competes with erythrocyte ghost membranes (spectrin-depleted preparations) for spectrin binding; and (d) it binds to purified spectrin and fodrin molecules. Most importantly, in intact lymphoma cells this ankyrin-like protein is localized directly underneath the plasma membrane and is found to be preferentially accumulated beneath receptor cap structures as well as associated with a membrane-cytoskeleton complex preparation. It is proposed that the ankyrin-like 72-kD protein may play an important role in linking certain surface glycoprotein(s) to fodrin which, in turn, binds to actin filaments required for lymphocyte cap formation.
GENERALIZED DESIGN METHODOLOGY OF HIGHLY EFFICIENT QUAD-FURCATED PROFILED HORNS WITH LARGER APERTURES (2022)

International audience
Simian Immunodeficiency Virus Evades a Dominant Epitope-Specific Cytotoxic T Lymphocyte Response Through a Mutation Resulting in the Accelerated Dissociation of Viral Peptide and MHC Class I (2000)

The ability of an AIDS virus to escape from immune containment by selective mutation away from recognition by CTL was explored in simian immunodeficiency virus of macaques (SIVmac)-infected rhesus monkeys. CTL recognition of a previously defined common viral mutation in an immunodominant SIVmac Gag epitope was evaluated. CTL were assessed for their ability to recognize a SIVmac Gag protein with a single residue 2 (T --> A) replacement in the minimal epitope peptide bound by the MHC class I molecule Mamu-A*01. SIVmac Gag-specific CTL lysed Mamu-A*01+ target cells infected with recombinant vaccinia virus expressing the wild-type but not the mutant Gag protein. In addition, CTL recognized the mutant epitope peptide less efficiently than the wild-type virus peptide. In studies to determine the mechanism by which the mutant virus evaded CTL recognition, this peptide was shown to bind Mamu-A*01 in a manner that was indistinguishable from the wild-type peptide. However, experiments in which an increasing duration of delay was introduced between peptide sensitization of target cells and the assessment of these cells as targets in killing assays suggest that the mutant peptide with a T --> A replacement had a higher off-rate from Mamu-A*01 than the wild-type peptide did. Therefore, these findings suggest that AIDS viruses can evade virus-specific CTL responses through the accelerated dissociation of mutant peptide from MHC class I.
Supplementary Figure S10 from Caspase-8 Promotes Cell Motility and Calpain Activity under Nonapoptotic Conditions (2023)

Supplementary Figure S10 from Caspase-8 Promotes Cell Motility and Calpain Activity under Nonapoptotic Conditions
Hitting the Target: Emerging Technologies in the Search for Kinase Substrates (2002)

Through phosphorylation, protein kinases can alter the activity, localization, protein association, and stability of their targets. Despite the importance to our understanding of all aspects of cell biology, progress toward identifying bona fide substrates of specific protein kinases has been slow. Traditionally used techniques to identify true kinase substrates, such as genetics, yeast two-hybrid screens, and biochemical purification, are often laborious and unreliable. However, several new approaches have recently been developed and used successfully to identify genuine in vivo substrates of certain protein kinases. These methods include screening for phosphorylation of proteins from phage expression libraries, peptide library screens to determine optimal motifs favored by specific kinases, the use of phospho-motif antibodies, and an approach that uses structurally altered kinases and allele-specific adenosine triphosphate analogs and kinase inhibitors. We describe these approaches and discuss their utility and inherent caveats.
Efficient Hydrogen Evolution by Mechanically Strained MoS2 Nanosheets (2014)

We demonstrated correlations between mechanically bent tensile-strain-induced two-dimensional MoS2 nanosheets (NSs) and their electrochemical activities toward the hydrogen evolution reaction (HER). The tensile-strain-induced MoS2 NSs showed significantly steeper polarization curves and lower Tafel slopes than the strain-free ones, which is consistent with the simple d-band model. Furthermore, the mechanical strain increased the electrochemical activities of all the NSs toward the HER except those loaded with high MoS2 mass. Mechanically bending MoS2 NSs to induce tensile strain enables the production of powerful, efficient electrocatalysis systems for evolving hydrogen.
Fast oxygen atom facility for studies related to low earth orbit activities (1992)

The operating principles and characteristics of an 8 km/s oxygen atom beam source are described. The source has been used for many applications relevant to low earth orbit (LEO) including both gas/surface and gas/gas interactions. Representative data from these activities are provided.
Quality control of extemporaneously prepared microdose captopril capsules: weight variation versus content uniformity. (1994)

Microdose (1 mg) captopril therapy is commonly used for the initial dose titration in patients with congestive heart failure. Since this dosage form is not commercially available, it has to be extemporaneously compounded. Quality control of each batch is commonly evaluated using the weight variation technique described in the USP. Despite careful titration with microdose, captopril capsules variability in patient's response has been observed. In order to explain this fluctuation, the actual content of extemporaneously compounded microdose captopril capsules was evaluated using a high pressure liquid chromatographic assay. Microdose captopril capsules were prepared by triturating 25 mg tablets with lactose in a mortar using standard geometric dilution technique and a Sharpe-Dohme hand-operated capsule filling machine. Forty-eight microdose captopril capsules were randomly selected from the compounded batch and were individually assayed for captopril amount. The mean +/- standard deviation (SD) amount of captopril in each capsule was 1.27 g +/- 0.31 mg with a range of 0.84 g to 1.96 mg. The coefficient of variation was 24.5%. Ten captopril capsules were randomly selected from the compounded batch and were individually weighed. The capsules had a mean weight +/- SD of 198.3 g +/- 21.2 mg and a coefficient of variation of 7.3%. Even though the extemporaneously prepared microdose captopril capsules were within acceptable limits for weight variation described in the USP, the actual dose administered to the patients could vary by as much as 24.5%.
Robust Optical Wireless Link for the Backhaul and Fronthaul of Small Radio Cells (2016)

This paper summarizes recent work on the use of optical wireless links as a robust outdoor backhaul solution for small radio cells, such as WiFi, LTE and 5G, over distances between 20 and 200 m. Results of a 5-month outdoor field trial in Berlin, Germany, indicate that the visibility range was never below 180 m, with more than 1 km in 99% of all times during this period. Rate-adaptive transmission proved to improve the availability remarkably in the occasional presence of fog or sunlight. A mathematical model of the backhaul link including these impairments is presented and implemented to obtain an optimized link design. A prototype was realized, accordingly, using low-cost optoelectronic components and a 1 Gb/s baseband chipset. Gross data rates of 800, 500, and 225 Mb/s have been achieved in real-time operation over 20, 100, and 215 m outdoor link distance, respectively, with 2 ms latency at 95% load. Finally, we discuss further evolution towards 5G mobile networks.
The Color of Shock Waves in Photonic Crystals (2004)

Unexpected and novel new physical phenomena result when light interacts with a shock wave or shock‐like dielectric modulation propagating through a photonic crystal, or material with a periodic modulation of the index of refraction. These theoretically predicted new phenomena include the capture of light at the shock wave front and re‐emission at a tunable pulse rate and carrier frequency across the photonic crystal bandgap, and bandwidth narrowing as opposed to the ubiquitous bandwidth broadening. To our knowledge, these effects do not occur in any other physical system. Reversed Doppler shifts are also predicted to be observable. Reversed Doppler shifts have never been observed in any system and have only been speculated to occur in pathological systems with simultaneously negative effective permittivity and permeability. The generality of these effects make them amenable to observation in a variety of time‐dependent photonic crystal systems, which may have significant technological implications.
Dominant Gain-of-Function Mutations in Hsp104p Reveal Crucial Roles for the Middle Region (2004)

Heat-shock protein 104 (Hsp104p) is a protein-remodeling factor that promotes survival after extreme stress by disassembling aggregated proteins and can either promote or prevent the propagation of prions (protein-based genetic elements). Hsp104p can be greatly overexpressed without slowing growth, suggesting tight control of its powerful protein-remodeling activities. We isolated point mutations in Hsp104p that interfere with this control and block cell growth. Each mutant contained alterations in the middle region (MR). Each of the three MR point mutations analyzed in detail had distinct phenotypes. In combination with nucleotide binding site mutations, Hsp104p(T499I) altered bud morphology and caused septin mislocalization, colocalizing with the misplaced septins. Point mutations in the septin Cdc12p suppressed this phenotype, suggesting that it is due to direct Hsp104p-septin interactions. Hsp104p(A503V) did not perturb morphology but stopped cell growth. Remarkably, when expressed transiently, the mutant protein promoted survival after extreme stress as effectively as did wild-type Hsp104p. Hsp104p(A509D) had no deleterious effects on growth or morphology but had a greatly reduced ability to promote thermotolerance. That mutations in an 11-amino acid stretch of the MR have such profound and diverse effects suggests the MR plays a central role in regulating Hsp104p function.
Langzeitüberleben trotz Pankreaskarzinom: Klinisch-pathologische Analyse prädiktiver Faktoren nach Resektion (2010)

Hintergrund: Das duktale Pankreaskarzinom ist mit einer sehr schlechten Prognose vergesellschaftet. Trotz dieser Tatsache gibt es durchaus Patienten, welche nach kurativ intendierter Resektion ein Langzeitüberleben aufweisen. Anhand eines großen Patientenkollektivs sollen prädiktive Faktoren zum Erlangen eines Langzeitüberlebens identifiziert werden.
Contribution à l'inventaire des Pézizales (Fungi, Ascomycota) du Lyonnais. 2e partie : taxinomie (2012)

Dans le cadre de l'inventaire des Pézizales du département du Rhône, l'auteur propose, dans cette seconde partie, la liste complète et commentée des taxons référencés jusqu'à ce jour. Les taxons critiques sont également énumérés à la fin de l'article.