gebhart/patrepeval huggingface dataset:
Hybridisation Potentials for Heavy Trucks Considering Route Topography (2016)

Based on dynamometer test cycles or plain motorway operation, heavy truck hybridisation must be considered as uneconomic if only the kinetic vehicle energy can be recuperated. In mountainous regions, micro hybridization by a 48V-belt generator or mild parallel hybridisation by a large high voltage electric drive can result in considerable fuel consumption savings as well as additional benefits for heavy load utility vehicles. Additional electric power and battery size are still critical design parameters as well as critical cost factors considering the limited space and depreciation time as well as the need for maximum payload. Based on vehicle model simulations, this contribution quantifies fuel consumption savings, recuperation energy harvesting and battery requirements for different truck sizes with test cycles based on realistic route topography. The main route topography parameter for the recuperation benefit is the effective incline that integrates all downhill sections that overcompensates the vehicle resistance by tire friction and air resistance. The simulation parameter studies lead to an analytical benefit estimation, based on load cycle parameters like effective velocity, effective incline as well as the vehicle parameters mass, drag coefficient and cross sectional area. Thus, the return on investment can be assessed by an analytic rule of thumb, based on tracked cycles of existing vehicles.
Organometallic palladium reagents for cysteine bioconjugation (2015)

Palladium(ii) complexes can be used in efficient and highly selective cysteine conjugation reactions that are rapid and robust, and the resulting aryl bioconjugates are stable towards acids, bases, oxidants and external thiol nucleophiles.
Preliminary results on LoRaWAN and IEEE 802.15.4-SUN Interference (2018)

We present some preliminary results on LoRaWAN and IEEE 802.15.4-SUN interference in urban environments. The results are based on a simple simulation that is parameterized using PHY layer measurements of controlled interference scenarios.
Tip-enhanced strong coupling spectroscopy, imaging, and control of a single quantum emitter (2019)

Optical cavities can enhance and control light-matter interactions. This level of control has recently been extended to the nanoscale with single emitter strong coupling even at room temperature using plasmonic nanostructures. However, emitters in static geometries, limit the ability to tune the coupling strength or to couple different emitters to the same cavity. Here, we present tip-enhanced strong coupling (TESC) with a nanocavity formed between a scanning plasmonic antenna tip and the substrate. By reversibly and dynamically addressing single quantum dots, we observe mode splitting up to 160 meV and anticrossing over a detuning range of ~100 meV, and with subnanometer precision over the deep subdiffraction-limited mode volume. Thus, TESC enables previously inaccessible control over emitter-nanocavity coupling and mode volume based on near-field microscopy. This opens pathways to induce, probe, and control single-emitter plasmon hybrid quantum states for applications from optoelectronics to quantum information science at room temperature.
Prolate Trochoidal Mass Spectrometer (2016)

This document contains recommendations for terminology in mass spectrometry. Development of standard terms dates back to 1974 when the IUPAC Commission on Analytical Nomenclature issued recommendations on mass spectrometry terms and definitions. In 1978, the IUPAC Commission on Molecular Structure and Spectroscopy updated and extended the recommendations and made further recommendations regarding symbols, acronyms, and abbreviations. The IUPAC Physical Chemistry Division Commission on Molecular Structure and Spectroscopy’s Subcommittee on Mass Spectroscopy revised the recommended terms in 1991 and appended terms relating to vacuum technology. Some additional terms related to tandem mass spectrometry were added in 1993 and accelerator mass spectrometry in 1994. Owing to the rapid expansion of the field in the intervening years, particularly in mass spectrometry of biomolecules, a further revision of the recommendations has become necessary. This document contains a comprehensive revision of mass spectrometry terminology that represents the current consensus of the mass spectrometry community.
Structure of the rabies virus glycoprotein trimer bound to a prefusion-specific neutralizing antibody (2022)

Rabies infection is nearly 100% lethal if untreated and kills more than 50,000 people annually, many of them children. Existing rabies vaccines target the rabies virus glycoprotein (RABV-G) but generate short-lived immune responses, likely because the protein is heterogeneous under physiological conditions. Here, we report the 3.39 Å cryo-electron microscopy structure of trimeric, prefusion RABV-G complexed with RVA122, a potently neutralizing human antibody. RVA122 binds to a quaternary epitope at the top of RABV-G, bridging domains and stabilizing RABV-G protomers in a prefusion state. RABV-G trimerization involves side-to-side interactions between the central α helix and adjacent loops, rather than contacts between central helices, and interactions among the fusion loops at the glycoprotein base. These results provide a basis from which to develop improved rabies vaccines based on RABV-G stabilized in the prefusion conformation.
Quantum Chemical and RRKM Investigation of the Elementary Channels of the Reaction C6H6 + O (3P) (2001)

We performed a computational study of an important reaction in the combustion of hydrocarbons, C6H6, + O (3P), using ab initio and RRKM methods. Density functional theory (B3LYP) was used to optimize geometries and obtain molecular vibrational frequencies, and complete basis set extrapolation (CBS-QB3) was used to obtain the energies for the reactants several transition states and products. The initial formation of a stabilized adduct is characterized by a barrier of 4.9 kcal mol-1, in good agreement with the measured activation energy for this reaction. All product channels originate from rearrangement or decomposition of this adduct, which our calculations suggest is a triplet ground state. All of our ab initio calculations are thus conducted on the triplet surface. There are several products that are energetically accessible at combustion temperatures, but the formation of phenoxy radical, C6H5O, and H atom dominate the product slate at low temperatures. Rearrangement to form formylcyclopentadiene, C5H5CHO, is also important at low temperatures, and the decomposition of this species to form cyclopentadienyl radical, C5H5, and HCO may be significant at higher temperatures. Rearrangement to form phenol is unimportant at all temperatures.
Re: Uropathogenic Escherichia coli Induces Chronic Pelvic Pain (2011)

No AccessJournal of UrologyInvestigative Urology1 Nov 2011Re: Uropathogenic Escherichia coli Induces Chronic Pelvic Pain Anthony AtalaM.D. Anthony AtalaAnthony Atala More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.07.053AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "Re: Uropathogenic Escherichia coli Induces Chronic Pelvic Pain." The Journal of Urology, 186(5), p. 2123 © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 186Issue 5November 2011Page: 2123 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Anthony Atala More articles by this author Expand All Advertisement PDF DownloadLoading ...
Enol Ether and Aldol Condensations (1996)

Since research on the total synthesis of carotenoids began, the enol ether and the aldol condensations have been frequently used for the formation of carbon-carbon double bonds. In general, the enol ether reaction has been employed successfully for the condensation of a wide range of acetals and sometimes of ketals with enol ethers and dienol ethers.
Does Tyrosine Protect S. Coelicolor Laccase from Oxidative Degradation? (2020)

We have investigated the roles of tyrosine (Tyr) and tryptophan (Trp) residues in the four-electron reduction of oxygen catalyzed by Streptomyces coelicolor laccase (SLAC). During normal enzymatic turnover in laccases, reducing equivalents are delivered to a type 1 Cu center (CuT1) and then are transferred over 13 Å to a trinuclear Cu site (TNC: (CuT3)2CuT2) where O2 reduction occurs. The TNC in SLAC is surrounded by a large cluster of Tyr and Trp residues that can provide reducing equivalents when the normal flow of electrons is disrupted. Canters and coworkers have shown that when O2 reacts with a reduced SLAC variant lacking the CuT1 center, a Tyr108· radical near the TNC forms rapidly. We have found that ascorbate reduces the Tyr108· radical in wild-type SLAC about 10 times faster than it reacts with the CuT12+ center, possibly owing to radical transfer along a Tyr/Trp chain. Aerobic oxidation of two reduced SLAC mutants (Y108F and W132F) leads to the formation of a long-lived (~15 min) Tyr· radical with distinct absorption at 408 nm. The diffusion of redox equivalents away from the primary enzymatic pathway in SLAC may indicate a poorly optimized enzyme or a mechanism to protect against protein damage.
Cushing disease in a patient with nonbullous congenital ichthyosiform erythroderma: lessons in avoiding glucocorticoids in ichthyosis (2019)

Nonbullous congenital ichthyosis erythroderma (CIE) is an autosomal recessive disorder of ineffective keratinization. We present a unique case of a 16-year-old female with CIE who developed Cushing disease (CD) at age 13 with concomitant worsening of her skin disease. After transsphenoidal resection of her pituitary adenoma, she had both resolution of her Cushing symptoms and significantly milder skin manifestations of her CIE. To the best of our knowledge, this is the first reported case of a patient with both CD and CIE, one that is important in demonstrating the role of glucocorticoids in this disorder.
Adalimumab combined with methotrexate versus adalimumab monotherapy in psoriasis: Three‐year follow‐up data of a single‐blind randomized controlled trial (2023)

In this small study, there was no significant difference in ADL overall drug survival when it was initially combined with MTX, compared to ADL alone. Discontinuation due to adverse events was common in the combination group. To secure accessible healthcare, combination treatment of ADL and MTX can be considered in individual patients.
Spectroscopic studies of upconverting chelates (2002)

We are investigating the spectroscopy of the new type of reporter for biomedical diagnostics called upconverting chelates. These compounds do not photobleach, have narrow emission bands, and are not affected by autofluorescence.
Staphylococcal enterotoxin B induces potent cytotoxic activity by intraepithelial lymphocytes (2000)

In food poisoning, Staphylococcus aureus secretes staphylococcal enterotoxin B (SEB), a superantigen that causes intense T-cell proliferation and cytotoxicity. The effects of SEB on lytic activity by human intestinal intraepithelial lymphocytes (IEL) were investigated. Jejunal IEL, from morbidly obese individuals undergoing gastric bypass operations, were tested for SEB-induced cytotoxicity against C1R B-lymphoblastoid cells, HT-29 adenocarcinoma cells, or CD1d-transfected cells using the 51Cr-release assay. Fas and Fas ligand expression were detected by immunofluorescence and flow cytometry and soluble ligand by enzyme-linked immunosorbent assay (ELISA). In the presence of SEB, IEL became potently cytotoxic against C1R cells and interferon-gamma (IFN-gamma)-precultured HT-29 cells, causing 55+/-10% and 31+/-6% lysis, respectively, greater than that by phytohaemagglutinin (PHA)-, interleukin-2 (IL-2)-, or anti-T-cell receptor (TCR)-activated IEL. SEB-stimulated peripheral blood (PB) CD8+ T cells lysed similar numbers of C1R cells but fewer HT-29 cells (53+/-13% and 8+/-5%, respectively). IEL killing of C1R cells involved interaction of major histocompatibility complex (MHC) class II with TCR, CD2 with CD58, and CD11a with CD54, and was perforin mediated. SEB-induced IEL lysis of HT-29 cells, in contrast, was caused by an unknown target cell structure, not MHC class II or CD1d, and resulted from a combination of perforin and Fas-mediated events. The potent cytotoxic activities of IEL promoted by SEB utilize two different mechanisms, depending on the surface receptors expressed by the target cells.
Combustion of premixed methane and air in ducts (2001)

Extinction limits are reported for flames stabilized on sudden expansions in plane and round ducts, with acoustically open and closed upstream ends, with and without downstream nozzles and, in the case of the round duct, with added swirl. Two main regimes of combustion oscillations were observed, one close to stoichiometry and the other close to the limits, and details of the corresponding flame structures were obtained from simultaneous measurements of pressure and imaging of the chemiluminescence of the CH radical. Similar results are presented for disc-stabilized flames.The pressure measurements in the unconstricted sudden-expansion flows show that combustion oscillations with equivalence ratios close to unity were associated with longitudinal acoustic frequencies, and the chemiluminescence images show the roll-up of vortices close to the pressure maximum followed by their growth and convection. With an open upstream end, the dominant acoustic mode was a half wave in the entire duct, and it changed to a quarter or three-quarter wave when the upstream end was acoustically closed. The addition of a downstream nozzle introduced a bulk-mode oscillation that dominated in the round duct but the large-amplitude oscillations were still associated with a longitudinal mode in the plane geometry: the quarter wave in the upstream duct this time. The pressure signals showed low-frequency modulations that increased with flow rate and, therefore, with heat release, and these were associated with flame movements caused by high strain rates.As the equivalence ratio was reduced toward the lean-extinction limit, the pressure signals fluctuated with periods of the order of 100 ms and the chemiluminescence showed that these were associated with cyclic longitudinal movements of the flame. These movements were caused by local extinction due to the high strain rates in the shear layer close to the stabilizer, with subsequent downstream translation of the flame until the strain rate reduced sufficiently to allow the flame to re-establish and return through the region of recirculation. The disc-stabilized flames gave rise to similar oscillations prior to extinction. Constricting the ducts with an exit nozzle narrowed the flammability range by ca. 30%, increased the amplitudes of the nearlimit oscillations by two orders of magnitude, and led to higher emissions of NOx.Attempts to suppress the near-limit oscillations in the sudden-expansion flows by imposing oscillations on the pressure field are described and reduced the amplitudes in the constricted ducts by up to 70% through suppression of the bulk-mode oscillation. They were less successful with near-stoichiometric oscillations and high flow rates, where the modulations presented problems that were not solved by active or open-loop control.Possible implications of the results for the operation of gas turbines are considered.
Survival of Campylobacter jejuni in Amoebae enhances subsequent invasion of mammalian cells (2021)

Abstract The ubiquitous unicellular eukaryote, Acanthamoeba , is known to play a role in the survival and dissemination of Campylobacter jejuni. C. jejuni is the leading cause of bacterial foodborne gastroenteritis world-wide and is a major public health problem. The ability of C. jejuni to interact and potentially invade epithelial cells is thought to be key for disease development in humans. We examined C. jejuni grown under standard laboratory conditions,11168H CBA with that harvested from within Acanthamoeba castellanii (11168H AC/CBA ) or Acanthamoeba polyphaga (11168H AP/CBA ), and compared their ability to invade different cell lines. C. jejuni harvested from within amoebae had a ∼3.7-fold increase in invasiveness into T84 human epithelial cells and a striking ∼11-fold increase for re-entry into A. castellanii cells. We also investigated the invasiveness and survivability of six diverse representative C. jejuni strains within Acanthamoeba spp ., our results confirm that invasion and survivability is likely host cell dependent. Our survival assay data led us to conclude that Acanthamoeba spp . are a transient host for C. jejuni and that survival within amoebae pre-adapts C. jejuni and enhances subsequent cell invasion. This study provides new insight into C. jejuni interactions with amoebae and its increased invasiveness potential in mammalian hosts.
Intrinsic Viscosity of Aqueous Solutions of Carboxymethyl Guar in the Presence and in the Absence of Salt (2008)

Abstract Intrinsic viscosities were determined for solutions of CMG in pure water and 0.9 wt.‐% aqueous NaCl. To avoid the 0/0‐type extrapolation typical for Huggins plots, a new procedure was used. For CMG and pure water, this requires only two adjustable parameters: the specific hydrodynamic volume of the polymer in the limit of infinite dilution and a hydrodynamic interaction parameter. The intrinsic viscosity of CMG (no salt) at room temperature is 6 050 mL · g −1 ; approximately half as large as that of Na‐PSS of comparable molar mass. The ratio of the intrinsic viscosities with and without salt is ≈7 for CMG, as compared to &gt;100 for Na‐PSS. The reasons for the different behaviors of the two types of polyelectrolytes are being discussed. magnified image
Supramolecular PEG−co-Oligo(p-benzamide)s Prepared on a Peptide Synthesizer (2006)

An automated synthesis protocol has been developed for the preparation of oligo(p-benzamide)s on solid support using a commercial peptide synthesizer employing a variation of standard Fmoc chemistry. Bis(trichloromethyl carbonate) in NMP was used to activate the aromatic carboxylic acids for acylation of secondary aromatic amines on solid support. N-Protected hepta(p-benzamide) was automatically prepared on solid support and manually converted to a solid supported block co-oligomer by attaching a poly(ethylene glycol) chain. Cleavage from the support could be achieved with minimal loss of the p-methoxybenzyl N-protective group. While the N-protected block co-oligomer was molecularly dissolved in nonpolar organic solvents, the N-deprotected block co-oligomer adopted a rod-coil conformation and showed strong aggregation as evidenced by gel permeation chromatography and transmission electron microscopy. Rigid rodlike aggregates could be observed in chloroform, toluene, as well as water.
Predictive Learning in the Presence of Heterogeneity and Limited (2014)

A large number of real-world domains possess heterogeneity in their data, which implies that different partitions of the data show different relationships between explanatory and response variables. This increases the overall model complexity of predictive learning in the presence of heterogeneity. Additionally, a number of real-world domains lack sufficient training data, making the learning algorithm prone to over-fitting, especially when the model complexity is large. However, there often exists a structure among the data instances and their partitions which can be appropriately leveraged for reducing the model complexity along with addressing heterogeneity. In this paper, we present a framework for learning robust predictive models in real-world heterogeneous datasets which lack sufficient number of training samples. We demonstrate the usefulness of our framework in the domain of remote sensing for forest cover estimation. Through a series of comparative experiments with baseline approaches, we are able to show that our framework: (a) captures meaningful information about heterogeneity in the data, (b) improves prediction performance by addressing data heterogeneity, (c) is robust to over-fitting in the presence of limited training data, and (d) is robust to the choice of the number of partitions used for representing heterogeneity.
Business Transformation Workbench (2011)

Business transformation is a key management initiative that attempts to align people, process and technology of an enterprise more closely with its business strategy and vision (Lee, 2005). Business transformation is an essential part of the competitive business cycle. Existing consulting methods and tools do not address issues such as scalability of methodology, data and knowledge management, method enforcement, asset reuse and governance, consolidated views of upstream and downstream analyses well, to name a few. This paper presents Business Transformation Workbench which is a practitioner’s tool for business transformation addressing these problems. It implements a methodical approach that was devised to analyze business transformation opportunities and make business cases for transformation initiatives and thereby provides decision-support to the consultants. The Business Transformation Workbench builds on a component-based model of a business and offers a consolidated view into clients’ operations, organization, staffing, processes and IT. It provides an intuitive way to evaluate and understand various opportunities in staff and IT consolidation and process standardization. It embodies structured analytical models, both qualitative and quantitative, to enhance the consultants’ practices. The Business Transformation Workbench has been instantiated with data from finance management domain and applied to address a client situation as a case study. An alpha testing of the tool was conducted with about dozen practitioners. The feedback has been encouraging. 90% of the consultants who tested the BT Workbench tool felt that the tool would help them do a better job during a client engagement. The tool is currently being piloted with customer engagements in a large IT consulting organization.Request access from your librarian to read this chapter's full text.
Curli Fibers Mediate Internalization of Escherichia coli by Eukaryotic Cells (2001)

Curli fibers are adhesive surface fibers expressed by Escherichia coli and Salmonella enterica that bind several host extracellular matrix and contact phase proteins and were assumed to have a role in pathogenesis. The results presented here suggest that one such role is internalization into host cells. An E. coli K-12 strain transformed with a low-copy vector containing the gene cluster encoding curli fibers (csg operon) was internalized by several lines of eukaryotic cells. The internalization could be correlated with a high level of curli fiber expression and was abolished by disruption of the csg operon. The ability to be internalized by eukaryotic cells could be conferred even by the curli fiber gene cluster of a noninvasive K-12 strain, but the homologous csg cluster from a virulent septicemic E. coli isolate mediated a higher level of internalization. The finding that curli fibers promote bacterial internalization indicates a new role for curli fibers in pathogenesis.
Supplementary Figures S1-S6 from Actin Cytoskeleton Remodeling Drives Breast Cancer Cell Escape from Natural Killer–Mediated Cytotoxicity (2023)

&lt;p&gt;Supplementary Figures S1-S6 and their legends&lt;/p&gt;