gebhart/patrepeval huggingface dataset:
E2F1 Activates the Human p53 Promoter and Overcomes the Repressive Effect of Hepatitis B Viral X Protein (HBx) on the p53 Promoter (2002)

The functional effect of the interaction of E2F1 and hepatitis B virus X protein (HBx) on the promoter of human p53 gene was studied using chloramphenicol acetyl transferase (CAT) assay. E2F1 activated the p53 promoter through E2F1 binding site. As previously reported, HBx repressed the p53 promoter through E-box. When E2F1 was cotransfected with HBx, E2F1 overcame the repressive effect of HBx on the p53 promoter through the E2F1 site. However, in the thymidine kinase (tk) heterologous promoter system with the E2F1 binding sites, cotransfection of E2F1 and HBx showed a strong synergistic activation. An in vitro interaction assay showed that E2F1 and HBx physically bind with each other. Analyses of the interaction domain with the GAL4 fusion protein showed that the pRb-binding domain of E2F1 was necessary for the functional interaction of these two proteins. Taken together, these results imply the functional inhibitory action of E2F1 on the HBV life cycle and HBV-mediated hepatocellular carcinogenesis (HCC). Therefore, the normal or enhanced function of E2F1 gene would be important in controlling the HBx function in HCC.
Development of Measuring System to Measure Standing Pose of the Foot Using Distributed Triaxial Force Sensor (2006)

The bottom of a person's foot grips the floor for balance, and the action force and action moment work at the foot bottom when he maintains posture and when he moves. They are important indices in the evaluation and the medical attention of standing pose balance and gait disturbances. A lot of equipment to measure the floor reaction force have been researched. However, no floor reaction force meter exists that can measure distribution information force in three directions. This paper aims at the development of a system that can measure the standing pose of the foot that exists from a measuring instrument and that can measure the standing pose of foot distributed 6×4 three axis force sensors and software that displays and preserves the output of the sensor element. A time change of force that worked at the foot bottom is sought as a vector by outputting each sensor element. Moreover, an action vector is three dimensionally displayed whose data can be intuitively understood. The results of experiments show that the measuring system can measure the action force of the foot bottom as distribution information on force in three directions.
Association of Levels of Fasting Glucose and Insulin With Rare Variants at the Chromosome 11p11.2- MADD Locus (2014)

Sequencing at 11p11.2-NR1H3 identified rare variation associated with FI. One variant, chr11:47227430, seems to be functional, with the rare A allele reducing transcription factor FOXA1 binding and FOXA1-dependent transcriptional activity.
1131 A Multifaceted Weight Loss Program for Management of Obesity Related Gastrointestinal Disorders in a Community Based Gastroenterology Practice (2019)

INTRODUCTION: Obesity related gastrointestinal disorders including Non-Alcoholic Fatty Liver Disease (NAFLD) and Gastroesophageal Reflux Disease (GERD) are more frequent and usually present earlier than diabetes mellitus type 2 and cardiovascular disorders. This provides an opportunity for the gastroenterologist to intervene early with an effective weight loss therapy. We evaluated the outcomes of a gastroenterologist supervised weight loss program aimed at achieving a 10% total body weight (TBW) loss at 3 months, a known correlation with NAFLD and GERD regression. METHODS: Patients with NAFLD and GERD seen at the clinic between May, 2017 to January, 2019 were offered participation in a medically supervised weight loss program comprised of a calorie restricted (800 Kcal/day) meal replacement diet, supervised medical fitness program, and weekly behavioral support groups for 3 months. This was followed by monthly follow up visits with slow reintroduction of whole food plant-based or Mediterranean diet. Two-sample t-Test assuming equal variances was used for statistical analysis. RESULTS: One hundred and fifty-three patients presented for their baseline office visit, 56 patients dropped out within 12 weeks of enrollment, and 97 patients with NAFLD (64 patients had both NAFLD and GERD) continue to participate in the program to date. At baseline, the 97 participants (68 Females), had a mean age of 56 years, and a mean BMI of 38 kg/m2. Average percentage weight loss was (4.1% ± 3.5%), (10.5% ± 4.7%), and (13.3% ± 6.5%) at 1 month, 3 months, and 6 months follow up respectively. At 3 months, 63% of patients had lost at least 10% of TBW. Furthermore, patients who attended &gt; 50% of the offered behavioral support groups lost 11.9% TBW at 3 months compared to 10.9% and 9.5% TBW loss for those who attended 10 - 50% and &lt; 10% respectively. Attending &gt; 50% of behavioral support groups was associated with a statistically significant higher TBW loss at 3 months when compared to no attendance (11.9% versus 10%, P -value = 0.042). CONCLUSION: Over 60% of patients who completed a 3 month follow up in a multifaceted, community based, gastroenterologist supervised, weight loss program have achieved a clinically significant TBW loss of at least 10%. Our results also suggest that incorporating weekly behavioral support groups into the program might be a useful adjunct to achieving a clinically significant weight loss in this patient population. Studies are ongoing to determine the sustainability of this weight loss over 1 year.
Enhanced Photodynamic Therapy Effects of Graphene Quantum Dots Conjugated with Aminoporphyrins (2021)

Porphyrins are promising materials for photodynamic therapy, but their low solubility and aggregation in biological environments are still obstacles to surpass. In order to overcome these limitations, the conjugation of porphyrins with graphene quantum dots (GQDs), here functioning as a vehicle to improve the internalization of porphyrins by cancer cells, was considered. The GQDs were conjugated with an aminoporphyrin via amide linkage using both thionyl chloride (SOCl2) and 1-ethyl-3-(3′-dimethylaminopropyl)carbodiimide coupling methodologies. Based on structural characterization (Fourier transform infrared and X-ray photoelectron spectroscopy), the best porphyrin loading was observed with the SOCl2 procedure. The new hybrids were investigated as phototherapeutic agents in high incidence breast cancer (T-47D cell line) under white light, and a significant photocytotoxic effect was observed at 10 nM. The conjugation of GQDs to the aminoporphyrin promoted an efficient uptake by the T-47D cells when compared with the nonimmobilized porphyrin. These results point out the high potential of the new hybrids to be explored as theragnostic agents.
Nucleic Acids:  Structures, Properties, and Functions By Victor A. Bloomfield, Donald M. Crothers, and Ignacio Tinoco, Jr., with contributions from John E. Hearst, David E. Wemmer, Peter A. Kollman, and Douglas H. Turner. University Science Books, Sausalito, CA. 2000. ix + 794 pp. 17 × 25 cm. ISBN 0-935702-49-0. $85.00. (2000)

ADVERTISEMENT RETURN TO ISSUEPREVBook ReviewNEXTNucleic Acids: Structures, Properties, and Functions By Victor A. Bloomfield, Donald M. Crothers, and Ignacio Tinoco, Jr., with contributions from John E. Hearst, David E. Wemmer, Peter A. Kollman, and Douglas H. Turner. University Science Books, Sausalito, CA. 2000. ix + 794 pp. 17 × 25 cm. ISBN 0-935702-49-0. $85.00.Sean Michael KerwinView Author Information Division of Medicinal Chemistry College of Pharmacy Institute for Cellular and Molecular Biology The University of Texas at Austin Austin, Texas 78712Cite this: J. Med. Chem. 2000, 43, 24, 4721–4722Publication Date (Web):October 13, 2000Publication History Published online13 October 2000Published inissue 1 November 2000https://pubs.acs.org/doi/10.1021/jm000396phttps://doi.org/10.1021/jm000396pbook-reviewACS PublicationsCopyright © 2000 American Chemical SocietyRequest reuse permissionsArticle Views1051Altmetric-Citations5LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Molecular structure,Nucleic acid structure,Nucleic acids Get e-Alerts
ETHNIC FOOD HABITS (1983)

Any discussion of minority ethnic group food habits would be incomplete without including the Chinese who have a food culture very different from the indigenous culture; a food culture which has remained virtually unchanged, regardless of the period of time spent in Britain. From the beginning the Chinese have been involved in the catering industry and although very nearly every High Street in the country has a Chinese restaurant or take‐away food shop, the majority know very little about the Chinese or the role of food in Chinese culture.
BAD-LAMP controls TLR9 trafficking and signalling in human plasmacytoid dendritic cells (2017)

Toll-like receptors (TLR) are essential components of the innate immune system. Several accessory proteins, such as UNC93B1, are required for transport and activation of nucleic acid sensing Toll-like receptors in endosomes. Here, we show that BAD-LAMP (LAMP5) controls TLR9 trafficking to LAMP1<sup>+</sup> late endosomes in human plasmacytoid dendritic cells (pDC), leading to NF-κB activation and TNF production upon DNA detection. An inducible VAMP3<sup>+/</sup>LAMP2<sup>+/</sup>LAMP1<sup>-</sup> endolysosome compartment exists in pDCs from which TLR9 activation triggers type I interferon expression. BAD-LAMP-silencing enhances TLR9 retention in this compartment and consequent downstream signalling events. Conversely, sustained BAD-LAMP expression in pDCs contributes to their lack of type I interferon production after exposure to a TGF-β-positive microenvironment or isolation from human breast tumours. Hence, BAD-LAMP limits interferon expression in pDCs indirectly, by promoting TLR9 sorting to late endosome compartments at steady state and in response to immunomodulatory cues.TLR9 is highly expressed by plasmacytoid dendritic cells and detects nucleic acids, but to discriminate between host and microbial nucleic acids TLR9 is sorted into different endosomal compartments. Here the authors show that BAD-LAMP limits type 1 interferon responses by sorting TLR9 to late endosomal compartments.
Control of epithelial cell structure and developmental fate: Lessons from Helicobacter pylori (2008)

Valuable insights into eukaryotic regulatory circuits can emerge from studying interactions of bacterial pathogens such as Helicobacter pylori with host tissues. H. pylori uses a type IV secretion system (T4SS) to deliver its CagA virulence protein to epithelial cells, where much of it becomes phosphorylated. CagA's phosphorylated and non-phosphorylated forms each interact with host regulatory proteins to alter cell structure and cell fate. Kwok and colleagues showed that CagA destined for phosphorylation is delivered using host integrin as receptor and H. pylori's CagL protein as an integrin-specific adhesin, and that CagL-integrin-binding activates the kinase cascade responsible for CagA phosphorylation. This research contributes to understanding infectious disease and the control of cell fates.
Evaluating the Use of Data Transformation for Information Visualization (2008)

Data transformation, the process of preparing raw data for effective visualization, is one of the key challenges in information visualization. Although researchers have developed many data transformation techniques, there is little empirical study of the general impact of data transformation on visualization. Without such study, it is difficult to systematically decide when and which data transformation techniques are needed. We thus have designed and conducted a two-part empirical study that examines how the use of common data transformation techniques impacts visualization quality, which in turn affects user task performance. Our first experiment studies the impact of data transformation on user performance in single-step, typical visual analytic tasks. The second experiment assesses the impact of data transformation in multi-step analytic tasks. Our results quantify the benefits of data transformation in both experiments. More importantly, our analyses reveal that (1) the benefits of data transformation vary significantly by task and by visualization, and (2) the use of data transformation depends on a user's interaction context. Based on our findings, we present a set of design recommendations that help guide the development and use of data transformation techniques.
Fouling of an anion exchange chromatography operation in a monoclonal antibody process: Visualization and kinetic studies (2013)

Fouling of chromatographic resins over their operational lifetimes can be a significant problem for commercial bioseparations. In this article, scanning electron microscopy (SEM), batch uptake experiments, confocal laser scanning microscopy (CLSM) and small-scale column studies were applied to characterize a case study where fouling had been observed during process development. The fouling was found to occur on an anion exchange (AEX) polishing step following a protein A affinity capture step in a process for the purification of a monoclonal antibody. Fouled resin samples analyzed by SEM and batch uptake experiments indicated that after successive batch cycles, significant blockage of the pores at the resin surface occurred, thereby decreasing the protein uptake rate. Further studies were performed using CLSM to allow temporal and spatial measurements of protein adsorption within the resin, for clean, partially fouled and extensively fouled resin samples. These samples were packed within a miniaturized flowcell and challenged with fluorescently labeled albumin that enabled in situ measurements. The results indicated that the foulant has a significant impact on the kinetics of adsorption, severely decreasing the protein uptake rate, but only results in a minimal decrease in saturation capacity. The impact of the foulant on the kinetics of adsorption was further investigated by loading BSA onto fouled resin over an extended range of flow rates. By decreasing the flow rate during BSA loading, the capacity of the resin was recovered. These data support the hypothesis that the foulant is located on the particle surface, only penetrating the particle to a limited degree. The increased understanding into the nature of the fouling can help in the continued process development of this industrial example.
Angled XPS Analysis of Low-k Dielectric Surfaces after Cleaning (2005)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.
NVIDIA FLARE: Federated Learning from Simulation to Real-World (2022)

Federated learning (FL) enables building robust and generalizable AI models by leveraging diverse datasets from multiple collaborators without centralizing the data. We created NVIDIA FLARE as an open-source software development kit (SDK) to make it easier for data scientists to use FL in their research and real-world applications. The SDK includes solutions for state-of-the-art FL algorithms and federated machine learning approaches, which facilitate building workflows for distributed learning across enterprises and enable platform developers to create a secure, privacy-preserving offering for multiparty collaboration utilizing homomorphic encryption or differential privacy. The SDK is a lightweight, flexible, and scalable Python package. It allows researchers to apply their data science workflows in any training libraries (PyTorch, TensorFlow, XGBoost, or even NumPy) in real-world FL settings. This paper introduces the key design principles of NVFlare and illustrates some use cases (e.g., COVID analysis) with customizable FL workflows that implement different privacy-preserving algorithms. Code is available at https://github.com/NVIDIA/NVFlare.
ChemInform Abstract: Hydrogen Bonding and Molecular Recognition: Synthetic, Complexation, and Structural Studies on Barbiturate Binding to an Artificial Receptor (1992)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Mesoporous Silica Nanoparticles with pH-Sensitive Nanovalves for Delivery of Moxifloxacin Provide Improved Treatment of Lethal Pneumonic Tularemia (2015)

We have optimized mesoporous silica nanoparticles (MSNs) functionalized with pH-sensitive nanovalves for the delivery of the broad spectrum fluoroquinolone moxifloxacin (MXF) and demonstrated its efficacy in treating Francisella tularensis infections both in vitro and in vivo. We compared two different nanovalve systems, positive and negative charge modifications of the mesopores, and different loading conditions-varying pH, cargo concentration, and duration of loading-and identified conditions that maximize both the uptake and release capacity of MXF by MSNs. We have demonstrated in macrophage cell culture that the MSN-MXF delivery platform is highly effective in killing F. tularensis in infected macrophages, and in a mouse model of lethal pneumonic tularemia, we have shown that the drug-loaded MSNs are much more effective in killing F. tularensis than an equivalent amount of free MXF.
P‐1: Uni‐Color Column Line Pentile‐Type Pixel Arrangement Design for Low Driving Power Consumption AMOLED Displays (2022)

As active‐matrix organic light emitting diode (AMOLED) displays have rapidly become the primary choice for high performance mobile devices, reducing their power consumption to enable long battery life continues to be a very important challenge. In particular, the power consumption required to drive the display electronic circuits has recently increased significantly due to the industry trends for high resolution, high refresh rates, and high screen aspect ratio. In this paper, we propose a uni‐color pixel column design for the typical Pentile‐type pixel arrangement used for AMOLED displays in order to lower the driving power consumption. Considering a QHD+, 6.x inch‐diagonal smartphone application, the estimated power saving benefits of the proposed architecture are 10% for averaged image contents, and up to 42% for red/blue saturated images in the analog driving power consumption.
Faculty Opinions recommendation of Discovery of 2,6-Dimethylpiperazines as Allosteric Inhibitors of CPS1. (2020)

Carbamoyl phosphate synthetase 1 (CPS1) is a potential synthetic lethal target in LKB1-deficient nonsmall cell lung cancer, where its overexpression supports the production of pyrimidine synthesis. In other cancer types, CPS1 overexpression and activity may prevent the accumulation of toxic levels of intratumoral ammonia to support tumor growth. Herein we report the discovery of a novel series of potent and selective small-molecule inhibitors of CPS1. Piperazine 2 was initially identified as a promising CPS1 inhibitor through a high-throughput screening effort. Subsequent structure-activity relationship optimization and structure-based drug design led to the discovery of piperazine H3B-616 (25), a potent allosteric inhibitor of CPS1 (IC50 = 66 nM).Copyright © 2020 American Chemical Society. PMID: 32551016
ChemInform Abstract: 4′‐Aminomethyl‐2,2′‐bipyridyl‐4‐carboxylic Acid (Abc) and Related Derivatives: Novel Bipyridine Amino Acids for the Solid‐Phase Incorporation of a Metal Coordination Site within a Peptide Backbone. (2000)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Prior Cryopreservation of Ex Vivo-Expanded Cord Blood Cells Is Not Detrimental to Engraftment as Measured in the NOD-SCID Mouse Model (2001)

Cytokine-mediated expansion has been proposed and successfully used to facilitate engraftment post transplantation. This study examined whether cryopreservation following expansion has a detrimental effect on the ability of cells to engraft, using the NOD-SCID mouse model. Cord blood (CB) CD34(+) cells were incubated for 7 days with stem cell factor (SCF), flt-3 ligand (FL), and megakaryocyte growth and development factor (MGDF). Expanded CD34(+) cells were transplanted into NOD-SCID mice either fresh or following cryopreservation and thawing. After thawing, recovery of nucleated cells was 94%, of CD34 cells was 63%, and of day-14 progenitors was 17%. The loss of day-14 progenitor cells among the thawed expanded cells did not influence the kinetics of human engraftment in the mouse. Bone marrow (BM) of mice transplanted with thawed expanded CD34(+) cells (14 +/- 3.9%) showed significantly higher levels of human engraftment than mice transplanted with fresh expanded CD34(+) cells (1.5 +/- 0.5%, p = 0.0064). Thawed expanded CD34(+) cells had significantly higher SCID Engrafting Potential (SEP) than freshly expanded CD34(+) cells (p < 0.001). Results suggest that prior cryopreservation does not prevent expanded cells engrafting in NOD-SCID mice.