gebhart/patrepeval huggingface dataset:
ADULT ENDOPYELOTOMY: IMPACT OF ETIOLOGY AND ANTEGRADE VERSUS RETROGRADE APPROACH ON OUTCOME (1998)

Antegrade endopyelotomy is the preferred approach in patients with primary ureteropelvic junction obstruction and concomitant renal calculi (13.4% of cases), and may also be preferable in patients with high insertion obstruction (6.7%). For all other primary and all secondary ureteropelvic junction obstruction, antegrade and retrograde endopyelotomy is effective therapy yet retrograde endopyelotomy results in less operating room time, shorter hospital stay, fewer complications and significantly less expense to achieve the desired outcome.
Sodium Channel Blockers (2017)

ADVERTISEMENT RETURN TO ISSUEPREVPatent HighlightNEXTSodium Channel BlockersBenjamin Blass*View Author Information Temple University School of Pharmacy, 3307 North Broad Street, Philadelphia, Pennsylvania 19140, United States*Tel: 215-707-1085. E-mail: [email protected]Cite this: ACS Med. Chem. Lett. 2017, 8, 3, 275–277Publication Date (Web):February 23, 2017Publication History Received2 February 2017Published online23 February 2017Published inissue 9 March 2017https://pubs.acs.org/doi/10.1021/acsmedchemlett.7b00048https://doi.org/10.1021/acsmedchemlett.7b00048editorialACS PublicationsCopyright © 2017 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views1133Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (646 KB) Get e-AlertscloseSUBJECTS:Alkyls,Group 17 compounds,Neurophysiology,Sodium,Substituents Get e-Alerts
Nuclear and cytoplasmic AID in extrafollicular and germinal center B cells (2006)

Activation-induced cytidine deaminase (AID) is necessary for immunoglobulin somatic hypermutation (SHM) and class switch recombination (CSR) in T-dependent immune response in germinal centers (GCs). The structural similarity of AID with RNA-editing enzymes and its largely cytoplasmic location have fueled controversial views of its mode of interaction with DNA. We show that AID, a mature B-cell-restricted cytoplasmic antigen, is relocated into the nucleus in 2.5% of CDKN1B(-), CCNB1(-) GC cells. The GC dark zone and the outer zone (OZ), but not the light zone, contain nuclear and cytoplasmic AID(+) blasts. AID(+) cells in the OZ are in contact with T cells and CD23(-) follicular dendritic cells. In addition, AID is expressed in extrafollicular large proliferating B cells, 14% of which have nuclear AID. GC and extrafollicular AID(+) cells express E47 but not the inhibiting BHLH protein Id2. Outside the GC, AID(+) B cells are in contact with T cells and show partial evidence of CD40 plus bcr stimulation-dependent signature (CCL22, JunB, cMYC, CD30) but lack early and late plasma cell markers. The distribution of nuclear AID is consistent with the topography of SHM and CSR inside the GC and in extrafollicular activated B cells.
Genotoxicity of chromium (III) and cobalt (II) and interactions between them (2021)

Abstract Introduction. Chromium and cobalt are essential trace elements that are required only in a small amount, otherwise their excess can cause toxic effects. Aim. The aim of this study was to determine the effects of chromium (III) and cobalt (II) and their combinations on genotoxicity in human fibroblasts cells (BJ). Material and methods. In this work, comet and micronucleus assays were used. The BJ cells were exposed to chromium chloride and cobalt chloride at concentration ranges from 100 to 1400 µM. Mixtures of these elements were prepared so as to examine interactions between them. Results. The present study shows the genotoxic effects of chromium (III) and cobalt (II) and their mixtures on BJ cells. In the comet assay, no comets were observed at the lowest concentrations; in the higher, a significant increase in their percentage was observed. In the other assay (formation of micronuclei), a statistically significant increase in the number of cells with micronuclei was observed in the BJ cells spiked with cobalt chloride and chromium chloride. In the case of simultaneous incubation of chromium chloride at 200 µM and cobalt chloride at 1000 µM in the BJ line, antagonism was observed. However, the interaction of chromium chloride at the 1000 µM and cobalt chloride at 200 µM leads to synergism between the studied elements. Conclusions. Cobalt (II) and chromium (III) show genotoxic properties, they induce breaks in double and single-stranded DNA and they cause formation of AP-sites that do not have purine or pyrimidine bases.
A Predictive Controller for Drivability and Comfort in Multi-Motor Electric Vehicles (2021)

We present a robust rate-based model predictive control (rate-based MPC) for controlling electric vehicle (EV) with independently actuated wheels and anti-squat/lift/dive suspensions that achieves drivability, i.e., good tracking performance, improved passenger comfort, i.e., reduction of chassis lift, pitch, and roll, and increased vehicle lateral stability. The controller is validated in closed-loop with CarSim demonstrating robustness to model errors between the high-fidelity CarSim simulation and the control-oriented prediction models.
Dynamic Gait Control of Biped Robot Based on Kinematics and Motion Description in Cartesian Space (1997)

This paper proposes a new dynamic gait control method of biped robot based on robust joint servo control. The method consists of two subjects. The first subject is the approximation of biped robot to the inverted pendulum for sagittal plane and lateral plane. The second subject is the constitution of dynamic gait control based on robust joint servo control and kinematics.The motion description in Cartesian space is determined from the motion of the inverted pendulum for sagittal plane and lateral plane. Suitability of the biped motion reference is confirmed by distribution of ZMP (Zero Moment Point). Using the inverse kinematics of biped robot, the biped motion references in Cartesian space is transformed to the position references in joint space. In joint space, the robust position control system consists of two-degrees-of-freedom control system based on coprime factorization and disturbance observer. Since the robust joint servo control system compensates the inertia variation and disturbance torque on dynamic gait control, this control system is suitable for the dynamic gait control of biped robot. The validity of the proposed method is confirmed by the experimental results.
Photopatternable silicone compositions for electronic packaging applications (2004)

A growing need for low stress high temperature thick film materials has prompted the development of new spin-coatable photopatternable silicones (Dow Corning WL-5000 series) to assist manufactures in building the next generation of electronic devices. These new negative-tone materials can be easily coated onto electronic substrates and patterned using standard i-line and broadband lithographic processes. Films ranging from 6 to 50 &mu;m have been demonstrated with patterned features resolved to an aspect ratio of less than 1.3. The etched regions provide a sloped sidewall and curved surfaces to facilitate metallization processes. The films are cured at low temperatures (150 to 250°C) to provide low modulus values in the range of 150 to 500 MPa, are inherently hydrophobic, and are based on cure chemistry that is acid free and delivers thermally stable cross-links. As a result, the films show very little shrinkage during thermal cure (~2%), do not require extended high temperature processing, and provide a very low residual stress (<8 MPa). They also show excellent thermal stability and mechanical integrity when exposed to high temperatures. A simple wet process has been developed to facilitate film rework and allow for sacrificial layer applications.
Methods of Isozyme Electrophoresis forTrichodermaandGliocladiumSpecies (1988)

Species of Trichoderma and the closely related genus Gliocladium are ubiquitous, active components of the soil microflora. As biocontrol agents, these fungi can protect a wide range of agronomic, horticultural, and ornamental plants against a variety of plant pathogens (6, 13), and a few strains have been patented for commercial use. Some strains enhance plant growth or rooting of cuttings even in the absence of pathogens (4, 14). Trichoderma spp. have also been studied extensively as potential sources of cellulases for utilization of lignocellulosic materials (10, 17). The taxonomy, genetics and population composition of these fungi are poorly understood. Limited knowledge of variability in these fungi and infrequent culturing of their sexual stages make delineation of narrowly defined species difficult (16). Additionally, little is known about such genetic components as ploidy levels, frequency of heterokaryosis, or the prevalence of parasexual events. Finally, methods are lacking to differentiate among strains for patent purposes, or to determine the variability and abundance of strains in natural ecosystems. Isozyme analysis by starch gel electrophoresis is a powerful method for taxonomic, genetic and population studies. Descriptions of general techniques and of numerous enzyme assay systems have been published (9, 12, 18, 20, 21). However, for studies of specific organisms, suitable enzyme assays and corresponding electrophoretic buffers must be determined empirically (8, 12, 18). For an enzyme assay to be useful, enzyme activity must be reliably detected and activity bands must be clearly resolved. In addition, polymorphism must exist among isolates of the study population. Also, the usefulness of an enzyme assay for a particular study often depends on the number of loci detected. Assays detecting products of single loci can be particularly useful because polymorphism among individuals reflects allelic differences, and electrophoretic variants can be scored as allozymes. The genetic basis of an isozyme phenotype usually can be inferred from the complexity of the activity band pattern visualized (9, 12, 18). In this study, 63 enzyme assays were tested in combination with four electrophoretic buffers to assess detection of enzyme activity, resolution of activity bands, and complexity ofphenotypes visualized. This report summarizes the most useful systems for studies of Trichoderma and Gliocladium spp. and identifies those which apparently visualize products of single loci, i.e., allo-
High-precision isotope-shift measurement of23S-23Ptransition in helium (1991)

The isotope shift for the ${2}^{3}$S-${2}^{3}$P transition between $^{3}\mathrm{He}$ and $^{4}\mathrm{He}$ has been measured with a laser-microwave spectroscopy technique to a precision of 1 ppm, or 30 kHz. Our result is 100 times more precise than previous measurements and is by far the most precise isotope-shift measurement ever made for a multielectron system. It provides, for the first time, not only a check for the specific mass shift, but also for the calculations of the nuclear volume shift, the relativistic corrections involving the coupling between electrons, and the QED contributions.
Electric utility fossil fuels beyond 1985 (1978)

Numerous research and development studies sponsored by the Electric Power Research Institute (EPRI) identified candidate coal-liquefaction processes for commercialization beginning in the mid-1980's, i.e., the H-Coal and Exxon processes to produce liquid turbine fuels and distillate and heavy boiler fuels (DBF and HBF), the donor and solvent processes for DBF and HBF, and the Solvent-Refiner-Coal (SRC)-II process for DBF. The estimated cost of these raw coal liquids is $3.25-$4.50/million Btu, EPRI, and the U.S. Department of Energy have been developing an SRC process to produce a clean substitute coal-derived solid fuel which could serve as a retrofit fuel for existing coal-fired plants. Kerr-McGee's critical solvent deashing and mild hydrotreating are best suited for the desulfurization of this fuel to < 0.9% sulfur. Coal gasification integrated with combined-cycle or gas-turbine powerplants can reduce baseload power cost and improve the thermal efficiency of the plants, but its commercialization is not likely until the 1990's. Due to the reduced NO/sub x/ emissions, methanol may have promise as a fuel for peaking turbine service despite its high cost (currently about $6/million Btu). This cost could be reduced by 10% by using the new liquid-phase methanol reactor developed by EPRI. Coal pyrolysis to liquid fuelmore » shows little incentive due to the low quality of the raw product.« less
From quantum-state-specific dynamics to reaction rates: the dominant role of translational energy in promoting the dissociation of D2 on Cu(111) under equilibrium conditions (1993)

We have calculated the rate of adsorption of isotropic D2 gas on a Cu(111) surface, using recently determined differential adsorption probabilities, as a function of translational energy, angle of incidence, and surface temperature for molecules in each vibrational–rotational state. If the D2 gas is at the same temperature, T, as the surface, the mean probability of dissociation per collision, 〈S0〉, is calculated to increase rapidly with temperature. Arrhenius plots of 〈S0〉vs. 1/T are in good qualitative agreement with measurements for hydrogen dissociation on Cu, but display a distinct curvature over the range 300–1000 K. A detailed analysis of this temperature dependence reveals that the increase in 〈S0〉 with T is due almost entirely to the increase in translational energy of the incident molecules. Increases in the populations of vibrationally or rotationally excited molecules are relatively unimportant, as are the changes in the adsorption with surface temperature.
NASA-ONERA Collaboration on Human Factors in Aviation Accidents and Incidents (2012)

This is the first annual report jointly prepared by NASA and ONERA on the work performed under the agreement to collaborate on a study of the human factors entailed in aviation accidents and incidents, particularly focused on the consequences of decreases in human performance associated with fatigue. The objective of this agreement is to generate reliable, automated procedures that improve understanding of the levels and characteristics of flight-crew fatigue factors whose confluence will likely result in unacceptable crew performance. This study entails the analyses of numerical and textual data collected during operational flights. NASA and ONERA are collaborating on the development and assessment of automated capabilities for extracting operationally significant information from very large, diverse (textual and numerical) databases; much larger than can be handled practically by human experts.
Studies of the Generation and Pericyclic Behavior of Cyclic Pentadienyl Carbanions. Alkylation Reactions as an Efficient Route to Functionalized cis-Bicyclo[3.3.0]octenes (2006)

Carbolithiation has been studied with alkyllithium reagents in a series of six- through nine-membered 3-methylene-1,4-cycloalkadienes, efficiently producing the corresponding cyclic pentadienyl carbanions. These pentadienyl anions display unique reactivity, depending on ring size. Cyclooctadienyl anions readily undergo disrotatory electrocyclization to cis-bicyclo[3.3.0]octenyl systems, which are trapped with a variety of electrophiles to stereoselectively provide functionalized cis-bicyclo[3.3.0]octenes. The carbolithiation and electrocyclization processes are examined using low-temperature (1)H NMR experiments. An expedient synthesis of a linear triquinane illustrates this methodology. Electrocyclization of the corresponding cyclononadienyl anion requires unusually high temperatures (120 degrees C), and computational studies provide insights into this change in reactivity. Cycloheptadienyl and cyclohexadienyl anions, generated via carbolithiation, provide functionalized cycloheptadienes and cyclohexadienes upon electrophilic capture. Trapping experiments reveal that the cycloheptadienyl anions are transformed to heptatrienyl anions. A series of experiments have been designed to explore evidence for the feasibility of equilibration of open and closed anionic systems, and these studies report the first isolation of a cis-bicyclo[3.1.0]hexene derived from electrocyclization of a cyclohexadienyl carbanion.
Figure S5 from Site-Specific PEGylation of Anti-Mesothelin Recombinant Immunotoxins Increases Half-life and Antitumor Activity (2023)

&lt;p&gt;Biodistribution of LMB-249-PEG and SS1P&lt;/p&gt;
The Effect of Class A Scavenger Receptor Deficiency in Bone (2006)

Class A scavenger receptor (SR-A) is predominantly expressed by macrophages, and because osteoclasts are of monocyte/macrophage lineage, SR-A is of potential interest in osteoclast biology. In addition to modified low density lipoprotein uptake, SR-A is also important in cell attachment and signaling. In this study we evaluated the effect of SR-A deletion on bone. Knock-out animals have 40% greater body weight than wild type. Body composition analyses demonstrated that total lean and fat body mass were greater in knock-out animals, but there was no significant difference in percent fat and lean body mass. Bone mineral density and content were significantly greater in knock-out compared with wild type animals. Micro-computed tomography analyses confirmed that total volume, bone volume as well as trabecular number, thickness, and connectivity were significantly greater in knock-out mice. As expected, trabecular separation was greater in wild type mice. The phenotype appears to be explained by 60% fewer osteoclasts in females and 35% fewer in males compared to wild type mice with a paradoxical increase in nuclei/osteoclast in knock-out animals. Furthermore, there were no differences in adipocyte number and osteoblast number or activity. The addition of the soluble extracellular domain of SR-A to RAW264.7 cells stimulated a concentration-dependent increase in osteoclast differentiation that was receptor activator of nuclear factor-kappaB ligand (RANKL)-dependent. Soluble SR-A had no effect on cell proliferation in the presence of RANKL but stimulated a 40% increase in numbers in the absence of RANKL. We conclude that SR-A plays a role in normal osteoclast differentiation, suggesting a novel role for this receptor in bone biology.
Abstract 1507: Transcriptional profiles of CD14+ cells in situ in melanoma tumors reveal plasticity and localization dependent function (2019)

Mechanisms contributing to immunotherapy resistance in patients are the object of intense studies. Myeloid cells play a major role in tumors and include cells with different functions that can be grossly summarized as: (1) Antigen capture for presentation (dendritic cells, DCs) or for degradation (macrophages); (2) Tissue repair (macrophages) and (3) Effector function (mast cells, monocytes, granulocytes). However, the functional states of myeloid cells in human tumors are not completely understood. To study intact tumor microenvironments we have established a comprehensive approach for cellular and molecular analysis. Modular analysis of bulk RNAseq data of whole melanoma tissue sections stratified patient samples based on lymphocytic infiltrate but revealed inflammation and myeloid modules spread across samples. Polychromatic immunofluorescence and histocytometry showed that CD14+ cells represent the majority of the total tumor immune infiltrate. Furthermore, the majority of T cells present in melanoma tissue are in direct contact with CD14+ cells rather than melanoma cells. The distribution of CD14+ cells shows two distinct patterns: CD14+ cells within cancer nests (intratumoral) which are in close proximity to melanoma cells and are loaded with melanoma protein; CD14+ cells in the tissue surrounding cancer nests (stromal) which do not contain melanoma protein cargo and are organized in dense infiltrates. Using customized immunofluorescence-guided laser capture micro-dissection, we harvested CD14+ cells based on their tissue location and melanoma protein load for downstream analysis. We find that unsupervised clustering of transcriptomes groups cells by their tissue localization, i.e., intratumoral vs. stromal. Computational analysis, revealed distinct and replicable gene signatures associated with different pathways, in intratumoral and stromal CD14+ cells. Thus, the transcriptome differentiates the functional status of CD14+ cells related to their localization within tumor.Citation Format: Jan Martinek, Kyung In Kim, Te-Chia Wu, Victor Wang, Hannah M. Brookes, Lili Sun, Ananya Gulati, Joshy George, Philipp Henrich, Florentina Marches, Anthony Rongvaux, Michael Chiorazzi, Jeff Chuang, Paul Robson, Richard Flavell, Jacques Banchereau, Karolina Palucka. Transcriptional profiles of CD14+ cells in situ in melanoma tumors reveal plasticity and localization dependent function [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 1507.
Isolation and Properties of Mutants of Azotobacter chroococcum Defective in Aerobic Nitrogen Fixation (1985)

Mutants of Azotobacter chroococcum which showed unusually O2-sensitive N2 fixation when grown on sugars were isolated following mutagenesis with nitrosoguanidine or the transposon Tm1. These mutants we called Fos– (inability to fix N2 on sugars). Aerotolerant growth of Fos– mutants was, in general, restored by carboxylic acids, Ca2+ or a combined-N source. Two main groups of mutants were distinguished: (1) regulatory mutants which failed to synthesize nitrogenase under aerobic conditions and which were complemented by the Klebsiella pneumoniae nifA gene product; (2) mutants affected at different steps in intermediary metabolism. The latter strains exhibited low respiration rates or high apparent K s(O2) values (when grown on sucrose) compared with the parent strain. They seemed to be defective in respiratory protection of nitrogenase. The restoration of aerotolerant N2-dependent growth of Fos– mutants by carboxylic acids was correlated with their ability to induce a decrease in the apparent K s(O2) value; however, in both the mutant and the parent strains maximum respiration rates did not change significantly. The Ca2+ requirement for diazotrophic growth in A. chroococcum seems to be related to the capability of these organisms to fix N2 in air. Ca2+ might be required for high fluxes in the tricarboxylic acid cycle and might act at the level of phosphoenolpyruvate carboxylase, which is involved in the replenishment of the tricarboxylic acid cycle and/or at the level of tricarboxylic acid cycle enzymes per se. It is suggested that respiratory protection requires the maintenance of high respiratory rates even at low O2 tension.