gebhart/patrepeval huggingface dataset:
Supplementary Figure 1 from RKI-1447 Is a Potent Inhibitor of the Rho-Associated ROCK Kinases with Anti-Invasive and Antitumor Activities in Breast Cancer (2023)

&lt;p&gt;PDF file, 93K, RKI-1447 inhibits the phosphorylation of the ROCK substrates MLC-2 and MYPT-1 in human breast cancer cells. MDA-MB-231 and MDA-MB-468 breast cancer cells were treated with either vehicle or RKI-1447 for 1 hour and processed for western immunoblotting with the indicated antibodies as described under Materials and Methods.&lt;/p&gt;
Bo coil designs for in vivo delta relaxation enhanced MR in humans (2010)

Targeted MRI contrast agents have recently been developed that have the ability to bind to specific molecules in the body. In some cases, the relaxivity of the agent significantly increases upon binding. For example, MS-325 is an agent that binds to serum albumin in the blood [1,2]. One difficulty is that signal enhancement provided by these ‘smart’ contrast agents is dependent on the amount of both bound and unbound agent. Delta relaxation enhanced magnetic resonance (dreMR) is a new technology, which allows the specific detection of targeted agents only after they have chemically bound to their target molecule [3]. This method uses an insert electromagnet to modify the main magnetic field as a function of time in an otherwise conventional MR scanner. By interchanging the magnetic field strength during longitudinal recovery of magnetization between two distinct values, the signal provided by the bound agent can be separated from that provided by unbound agent. This is possible by taking advantage of the fact that certain novel MR contrast agents exhibit rapidly changing relaxivity as a function of magnetic field when in the bound state. Previous demonstrations of the dreMR technique have used quite small-bore electromagnet inserts to produce the required shifts in magnetic field. In this study, we investigate the design and performance of insertable electromagnets suitable for performing localized dreMR imaging in human subjects. The two particular anatomical areas of interest are the head/neck, and the prostate; however, this approach may be extended to a variety of other application areas.
Structure-activity relationships for the inhibition of acrosin by benzamidine derivatives (1978)

A series, consisting of 52 benzamidine derivatives, was evaluated for inhibitory activity against homogeneous boar sperm acrosin. All of the compounds in the series proved to be more potent than benzamidine (Ki = 4.0 x 10(-6) M), with one of the derivatives, alpha-(4-amidino-2,6-diiodophenoxy)-3-nitrotoluene (compound 16), showing outstanding potency with a Ki value of 4.5 X 10(-8) M. Although all of the derivatives were effective acrosin inhibitors, structural specificity was observed within homologous groups of compounds. The information gained from this preliminary study should prove extremely beneficial in the design and synthesis of future acrosin inhibitors.
Low-Temperature Spectroscopic Observation of Chain Growth and Migratory Insertion Barriers in (α-Diimine)Ni(II) Olefin Polymerization Catalysts (1999)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTLow-Temperature Spectroscopic Observation of Chain Growth and Migratory Insertion Barriers in (α-Diimine)Ni(II) Olefin Polymerization CatalystsSteven A. Svejda, Lynda K. Johnson, and Maurice BrookhartView Author Information Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-3290 Cite this: J. Am. Chem. Soc. 1999, 121, 45, 10634–10635Publication Date (Web):October 27, 1999Publication History Received10 June 1999Published online27 October 1999Published inissue 1 November 1999https://pubs.acs.org/doi/10.1021/ja991931hhttps://doi.org/10.1021/ja991931hrapid-communicationACS PublicationsCopyright © 1999 American Chemical SocietyRequest reuse permissionsArticle Views2245Altmetric-Citations267LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Adducts,Alkyls,Catalysts,Ethers,Hydrocarbons Get e-Alerts
High-Intensity Resistance Training Improves Glycemic Control in Older Patients With Type 2 Diabetes (2002)

High-intensity progressive resistance training, in combination with moderate weight loss, was effective in improving glycemic control in older patients with type 2 diabetes. Additional benefits of improved muscular strength and LBM identify high-intensity resistance training as a feasible and effective component in the management program for older patients with type 2 diabetes.
Impact of ionic liquid properties on selective enrichment of glycerides in direct lipase-catalyzed esterification (2016)

The direct lipase-catalyzed esterification of oleic acid and glycerol was studied in ionic liquids in order to deduce the effects of solvent properties such as viscosity, <italic>E</italic><sub>N</sub><sup>T</sup>, log <italic>P</italic> and Kamlet–Taft parameters on selective enrichment of glycerides.
Perspectives on Attenuation Estimation from Pulse-Echo Signals (1984)

Invited Paper Abstruct-Attenuation is an important parameter in the description prehensions of the physical and computational foundations of the propagation of an ultrasonic pulse in tissue. Since attenuation of the technioues. has been shown to be influenced by tissue state and pathology it also an important factor in the development of quantitative techniques for the measurement of ultrasonic tissue parameters and/or the display of ultrasonic tissue signatures (ultrasonic tissue characterization). Although attenuation is intrinsically defined in terms of a transmission measurement, it can be estimated from an analysis of the backscattered echo waveform. The development of such estimates is fundamentally a problem for digital signal processing. Recent advances in ultrasound instrumentation and computing technology have made the development of attenuation estimation from backscattering practical, thereby suggesting the feasibility of in vivo measurement of attenuation, and creating a popular and important area for research and development. Here we provide a perspective of the estimation of attenuation from pulse-echo signals, and overview the various attenuation estimation techniques proposed to date.
Characterization of Some EPDM-g-MA/OMMT Nanocomposites (2010)

(Nano)composites based on maleated ethylene propylene diene terpolymer (EPDM-g-AM) and organically modified montmorillonite (OMMT) has been prepared by melt intercalation procedure. The materials were characterized by X-ray diffraction (XRD), thermogravimetric analysis (TGA), dynamic scanning calorimetry (DSC) and mechanical tests. XRD data show the increase of the distance between the silicate layers indicating the intercalation of polymer chains in the montmorillonite galleries. The incorporation of OMMT in composites determines the increase of melting peak temperature (Tm) and heat of fusion (ΔHf) suggesting a supplementary nucleation increase due to the OMMT presence. The mechanical properties were analyzed as a function of the OMMT level in the composite and the results reveal remarkable improvement relative to the conventional composite.
Downregulation of the early genomic growth factor response in neu oncogene-transformed cells. (1990)

Peptide growth factor-induced signal transduction leads to a long-term adjustment of the genetic programs of responding cells. A point mutation in the transmembrane domain of the neu receptor has been found to activate its tyrosine kinase and oncogenic potential. Our previous studies show that ligand stimulation of a chimeric epidermal growth factor receptor-neu proto-oncogene (EGF-R/neu) induces the neu tyrosine kinase and leads to the programmed activation of cell growth-regulated genes. We have now studied the effect of the neu oncoprotein on the genomic growth factor response in cells expressing the EGF-regulated neu tyrosine kinase. Expression of the neu oncogene in these cells inhibited 75-90% of the EGF-stimulated mRNA induction of the immediate early serum response genes, such as junB encoding a transcription factor, N10 encoding a putative nuclear hormone binding receptor for an as yet undefined ligand, and B10, the protein product of which is still unknown. The relative lack of mRNA induction was not due to a loss of the chimeric EGF-R/neu receptors from the cell surface. Also, the neu oncogene decreased serum- and tumor promoter induction of these genes. Our results suggest that the neu oncogene is capable of deregulating mRNA responses to extracellular signalling, similar to the effects of the c-Ha-ras oncogene. Knowledge of the mechanisms responsible for these changes in gene regulation will help to define oncogenic transformation of cells in molecular terms.
Strain-specific differentiation of lactococci in mixed starter culture populations using randomly amplified polymorphic DNA-derived probes (1997)

A hydrophobic grid membrane filtration (HGMF) colony hybridization assay was developed that allows strain-specific differentiation of defined bacterial populations. The randomly amplified polymorphic DNA (RAPD) fingerprinting technique was used to identify potential signature nucleic acid sequences unique to each member of a commercial cheese starter culture blend. The blend consisted of two closely related Lactococcus lactis subsp. cremoris strains, 160 and 331, and one L. lactis subsp. lactis strain, 210. Three RAPD primers (OPX 1, OPX 12, and OPX 15) generated a total of 32 products from these isolates, 20 of which were potential strain-specific markers. Southern hybridization analyses revealed, that the RAPD-generated signature sequences OPX15-0.95 and a 0.36-kb HaeIII fragment of OPX1-1.0b were specific for strains 331 and 210, respectively, within the context of the test starter culture blend. These strain-specific probes were used in a HGMF colony hybridization assay. Colony lysis, hybridization, and nonradioactive detection parameters were optimized to allow specific differentiation and quantitation of the target strains in the mixed starter culture population. When the 210 and 331 probes were tested at their optimal hybridization temperatures against single cultures, they detected 100% of the target strain CFUs, without cross-reactivity to the other strains. The probes for strains 210 and 331 also successfully detected their targets in blended cultures even with a high background of the other two strains.
Reovirus Protein ςNS Binds in Multiple Copies to Single-Stranded RNA and Shares Properties with Single-Stranded DNA Binding Proteins (2000)

Reovirus nonstructural protein sigmaNS interacts with reovirus plus-strand RNAs in infected cells, but little is known about the nature of those interactions or their roles in viral replication. In this study, a recombinant form of sigmaNS was analyzed for in vitro binding to nucleic acids using gel mobility shift assays. Multiple units of sigmaNS bound to single-stranded RNA molecules with positive cooperativity and with each unit covering about 25 nucleotides at saturation. The sigmaNS protein did not bind preferentially to reovirus RNA over nonreovirus RNA in competition experiments but did bind preferentially to single-stranded over double-stranded nucleic acids and with a slight preference for RNA over DNA. In addition, sigmaNS bound to single-stranded RNA to which a 19-base DNA oligonucleotide was hybridized at either end or near the middle. When present in saturative amounts, sigmaNS displaced this oligonucleotide from the partial duplex. The strand displacement activity did not require ATP hydrolysis and was inhibited by MgCl(2), distinguishing it from a classical ATP-dependent helicase. These properties of sigmaNS are similar to those of single-stranded DNA binding proteins that are known to participate in genomic DNA replication, suggesting a related role for sigmaNS in replication of the reovirus RNA genome.
Disseminated Bartonella infection with granulomatous hepatitis in a liver transplant recipient (1999)

Disseminated infection with Bartonella spp with granulomatous hepatitis was diagnosed in a liver transplant recipient presenting with fever of unknown origin. Pathological findings on liver biopsy were atypical, with scant granulomas seen only after a second biopsy. The patient responded promptly to antibiotic therapy. Infections caused by Bartonella spp should be considered in transplant recipients with fever of unknown origin.
11107 Development of a Rehabilitation Robot Suit with Mechanical Safety Devices : Mechanical Design (2013)

Safety is one of the most important issues in rehabilitation robot suits. We present a new rehabilitation robot suit equipped with two mechanical safety devices. The robot suit assists a patient's knee joint. The safety devices consist of only mechanical components without actuators, controllers, or batteries. We expect that the safety devices guarantee the safety even if the computer does not work. In this paper, we firstly describe the rehabilitation robot suit with the two safety devices. Secondly, we show the basic structure of the robot suit. Finally, we explain the experiment device which we built in order to examine the usefulness of the two safety devices.
Hypermethylation can selectively silence individual p16ink4A alleles in neoplasia. (1998)

Inactivation of p16ink4A and other tumor suppressor genes has been associated with promoter region hypermethylation in neoplasia. However, direct proof for aberrant DNA methylation as an independent event for loss of gene function has been difficult to obtain. We addressed this question in the colon carcinoma cell line HCT116, which contains one allele of p16ink4A with a coding region frameshift mutation and one wild-type allele. Neither allele contains a mutation in the proximal promoter region. The promoter of the wild-type allele, but not the mutant allele, is hypermethylated, and only the mutant allele is expressed. Transcription from the methylated/wild-type allele was restored after cell treatment with the demethylating agent 5-aza-2'-deoxycytidine. Thus, in neoplastic cells, stable allele-specific loss of transcription may arise from aberrant methylation of a nonmutated promoter region, identifying hypermethylation as a direct mechanism for tumor suppressor gene inactivation.
Design and synthesis of highly active MoVTeNb-oxides for ethane oxidative dehydrogenation (2019)

Ethane oxidative dehydrogenation (ODH) is an alternative route for ethene production. Crystalline M1 phase of Mo-V mixed metal oxide is an excellent catalyst for this reaction. Here we show a hydrothermal synthesis method that generates M1 phases with high surface areas starting from poorly soluble metal oxides. Use of organic additives allows control of the concentration of metals in aqueous suspension. Reactions leading to crystalline M1 take place at 190 °C, i.e., approximately 400 °C lower than under current synthesis conditions. The evolution of solvated polyoxometalate ions and crystalline phases in the solid is monitored by spectroscopies. Catalysts prepared by this route show higher ODH activity compared to conventionally prepared catalysts. The higher activity is due not only to the high specific surface area but also to the corrugated lateral termination of the M1 crystals, as seen by atomic resolution electron microscopy, exposing a high concentration of catalytically active sites.
Multi-GPU Image-based Visual Hull Rendering (2012)

Many virtual mirror and telepresence applications require novel viewpoint synthesis with little latency to user motion. Image-based visual hull (IBVH) rendering is capable of rendering arbitrary views from segmented images without an explicit intermediate data representation, such as a mesh or a voxel grid. By computing depth images directly from the silhouette images, it usually outperforms indirect methods. GPU-hardware accelerated implementations exist, but due to the lack of an intermediate representation no multi-GPU parallel strategies and implementations are currently available. This paper suggests three ways to parallelize the IBVH-pipeline and maps them to the sorting classification that is often applied to conventional parallel rendering systems. In addition to sort-first parallelization, we suggest a novel sort-last formulation that regards cameras as scene objects. We enhance this method's performance by a block-based encoding of the rendering results. For interactive systems with hard real-time constraints, we combine the algorithm with a multi-frame rate (MFR) system. We suggest a combination of forward and backward image warping to improve the visual quality of the MFR rendering. We observed the runtime behavior of the suggested methods and assessed how their performance scales with respect to input and output resolutions and the number of GPUs. By using additional GPUs, we reduced rendering times by up to 60%. Multi-frame rate viewing can even be ten times faster.
ChemInform Abstract: A PHOTOCHEMICALLY MEDIATED (4C + 2C) ANNELATION. SYNTHESIS OF (.+‐.)‐10‐EPIJUNENOL (1978)

Abstract Die bei Bestrahlung von dem Cyclobuten (I) und Cyclopenten (II) erfolgte [2+2]‐ Photocycloaddition zu dem Addukt (III) ist als latente [4C+2C]Anellierung aufzufassen; unter milden thermischen Bedingungen (‐78 bis 20°C) können die exclusiv cis‐verknüpften Hydrindan‐Derivate (IV) bis (VI) erhalten werden.
T-Cell Recognition ofMycobacterium tuberculosisCulture Filtrate Fractions in Tuberculosis Patients and Their Household Contacts (1999)

We examined the immune responses of patients with active pulmonary tuberculosis (TB) and their healthy household contacts to short-term culture filtrate (ST-CF) of Mycobacterium tuberculosis or molecular mass fractions derived from it. Our goal was to identify fractions strongly recognized by donors and differences among the donor groups of possible relevance for vaccine development. The study population consisted of 65 human immunodeficiency virus-negative donors from the Hossana Regional Hospital, Hossana, Ethiopia. Peripheral blood leukocytes from the donors were stimulated with different antigens and immune responses were determined. Household contacts produced significantly higher levels of gamma interferon (IFN-gamma) than the TB patients in response to antigens present in ST-CF and the 10 narrow-molecular-mass fractions. A similar difference in leukocyte proliferative responses to the antigens between the two groups was also found. In general, while all fractions stimulated immune responses, the highest activity was seen with the low-molecular-mass fractions, which include well-defined TB antigens such as ESAT-6. Leukocytes from contacts of TB patients with severe disease produced higher levels of antigen-specific IFN-gamma than those from contacts of patients with minimal disease. Both groups of contacts exhibited higher cell-mediated responses than the patients themselves. The enhanced immune response of healthy contacts, especially those of patients with severe disease, to secreted mycobacterial antigens is suggestive of an early stage of infection by M. tuberculosis, which could in time result in overt disease or containment of the infection. This possibility is currently being investigated by follow-up studies of the household contacts.
Efficient “green” encapsulation of a highly hydrophilic anticancer drug in metal–organic framework nanoparticles (2015)

Metal-organic frameworks (MOFs) are coordination polymers of interest for biomedical applications. Of particular importance, nanoparticles made of iron(III) trimesate (MIL-100, MIL standing for Material Institut Lavoisier) (nanoMOFs) can be conveniently synthesised under mild and green conditions. They were shown to be biodegradable, biocompatible and efficient to encapsulate a variety of active molecules. We have addressed here the challenges to encapsulate a highly hydrophilic anticancer prodrug, phosphated gemcitabin (Gem-MP) known for its instability and inability to bypass cell membranes. MIL-100 nanoMOFs acted as efficient "nanosponges", soaking Gem-MP from its aqueous solution with almost perfect efficiency (>98%). Maximal loadings reached ∼30 wt% reflecting the strong interaction between the drug and the iron trimesate matrices. Neither degradation nor loss of crystalline structure was observed after the loading process. Storage of the loaded nanoMOFs in water did not result in drug release over three days. However, Gem-MP was released in media containing phosphates, as a consequence to particle degradation. Drug-loaded nanoMOFs were effective against pancreatic PANC-1 cells, in contrast to free drug and empty nanoMOFs. However, an efflux phenomenon could contribute to reduce the efficacy of the nanocarriers. Size optimization and surface modification of the nanoMOFs are expected to further improve these findings.
Designing toys with automatic play characterization for supporting the assessment of a child's development (2008)

In this paper, we describe the design considerations and implementation of the Child'sPlay system, a technology for supporting the automatic recording, recognition, and quantification of a child's object play behaviors for retrospective analysis. Our prototype system consists of six varieties of toys augmented with wireless sensing capabilities and a mobile computing platform which uses statistical pattern recognition techniques to automatically classify sensed play behaviors. This paper discusses our choices in toy design both in form factor as well as sensing capabilities. In addition, we also describe the play activities the system supports and provide an overview of our initial recognition algorithms.
Reversible Control Method for Particle Dispersion and Flocculation using Ionic Crosslinking of Polyelectrolytes (2018)

スラリー状製品における保存時の粒子の沈降・堆積・固化防止と塗布時の低粘性実現のため，形成される堆積層は非常に疎で，なおかつ弱い撹拌で良分散状態に戻り容易に流動可能となる軟凝集性スラリーの調製を試みた。本研究では，高分子電解質分散剤を用いていったん粒子を良分散状態とした後，多価イオンを添加して粒子表面の高分子分散剤をイオン架橋させた。これにより，低い降伏応力をもつ緩いゲルが形成され，軟凝集性スラリーの調製に成功した。このスラリーは，軽く振盪するだけで容易に流動状態へと移行し，時間が経過すれば再度ゲル状態に戻るという可逆性を有していた。さらに，ゲル化の度合いは添加する多価イオンの量で調整可能であった。
Use of an Escherichia coli beta-glucuronidase gene as a reporter gene for investigation of Bacteroides promoters (1991)

We have constructed transcriptional fusion vectors for use in Bacteroides spp., a genus of gram-negative obligate anaerobes found in high numbers in the human colon. The reporter group in these vectors is a promoterless beta-glucuronidase gene from Escherichia coli (uidA). Two of the vectors (pMJF-2 and pMJF-3) replicate in Bacteroides spp. The third, pCQW-1, does not replicate in Bacteroides spp. and can be used to introduce E. coli beta-glucuroindase fusions into the Bacteroides chromosome.