gebhart/patrepeval huggingface dataset:
Treatment of Atopic Dermatitis by Bone Marrow-derived Clonal Mesenchymal Stem Cells (119.3) (2012)

Abstract Mesenchymal stem cells (MSCs) can perform immunomodulatory activities, including suppression of T- and B-cell proliferation. MSCs have been primarily studied for their suppression of pro-inflammatory Th1 responses, but their therapeutic effects on atopic dermatitis (AD), which is a Th2-dominant disease, have not been reported. In this study, using an ovalbumin-induced mouse AD model, we investigated whether MSCs reduce the allergic inflammation in AD. We used mouse bone marrow-derived clonal mesenchymal stem cells (mcMSCs) isolated by our newly established isolation protocol termed the subfractionation culturing method (SCM). When injected intravenously into animals between antigen challenges, mcMSCs were found to inhibit the majority of AD-specific pathological changes by decreasing cell infiltration in skin lesions and reducing serum level of IgE. To explore the mechanism of this effect, we investigated the effects of mcMSCs on IgE production in B cells. We found that mcMSCs significantly inhibited IgE production when B cells were stimulated either by lipopolysaccharide (LPS) or anti-CD40 antibodies in the presence of interleukin (IL)-4. In addition, allogeneic mcMSCs can inhibit both isotype class switching and terminal differentiation of B cells probably by inhibiting NF-κB activation. These results suggest that mcMSC-based cell therapy is a promising therapeutic modality for AD treatment.
Tactile forward masking in Pacinian and non-Pacinian channels (1994)

Four subjects were tested with a forward masking paradigm to determine the time course of masking in the Pacinian (P) and a non-Pacinian (NPI) channel. Stimuli presented to the thenar eminence were either centered below 27 Hz or at 500 Hz to preferentially activate the NPI or P channels, respectively. Two-interval forced-choice tracking was used to measure thresholds. A masking stimulus was presented 100 ms after each interval onset and a test occurred in one of the two intervals at some specified delay (delay t) relative to the masking stimulus. Delta t was varied from 10 to 1000 ms and the masking stimulus level varied from 5 to 25 dB above thresholds in 5-dB steps. The results showed that the masking functions varied significantly with delta t. At longer delta t’s there was less masking that followed an exponential decay. The results from the individual channels differed from each other with respect to the slope of the masking functions and the exponential decay of masking with delta t. The time constants for the PC channel ranged from 20 to 40 ms, whereas the time constants for NPI ranged from 50 to 120 ms. [Work supported by NIH, NIDCD.]
From Waste Paper Basket to Solid State and Li‐HEC Ultracapacitor Electrodes: A Value Added Journey for Shredded Office Paper (2014)

Hydrothermal processing followed by controlled pyrolysis of used white office paper (a globally collectable shredded paper waste) are performed to obtain high surface area carbon with hierarchical pore size distribution. The BET specific surface area of such carbon is 2341 m(2) g(-1). The interconnected macroporous structure along with the concurrent presence of mesopores and micropores makes the material ideal for ultracapacitor application. Such waste paper derived carbon (WPC) shows remarkable performance in all solid-state supercapacitor fabricated with ionic liquid-polymer gel electrolyte. At room temperature, the material exhibits a power density of 19,000 W kg(-1) with an energy capability of 31 Wh kg(-1). The Li-ion electrochemical capacitor constructed using WPC as cathode also shows an excellent energy storage capacity of 61 Wh kg(-1).
Antiviral Properties of a Series of 1,6-Naphthyridine and 7,8-Dihydroisoquinoline Derivatives Exhibiting Potent Activity against Human Cytomegalovirus (2000)

A series of 1,6-naphthyridine (L. Chan, H. Jin, T. Stefanac, J. F. Lavallee, G. Falardeau, W. Wang, J. Bedard, S. May, and L. Yuen, J. Med. Chem. 42:3023-3025, 1999) and isoquinoline (L. Chan, H. Jin, T. Stefanac, W. Wang, J. F. Lavallee, J. Bedard, and S. May, Bioorg. Med. Chem. Lett. 9:2583-2586, 1999) analogues exhibiting a high level of anti-human cytomegalovirus (HCMV) activity were investigated in a series of studies aimed at better understanding the mechanism of action of some representatives of this class of compounds. In vitro antiviral profiling revealed that these compounds were active against a narrow spectrum of viruses, essentially the human herpesviruses and type 2 rhinovirus. In HCMV assays, a 39- to 223-fold lower 50% inhibitory concentration was obtained for compound A1 than for ganciclovir against strains AD 169 and Towne. In addition, ganciclovir, foscarnet, cidofovir, and BDCRB (2-bromo-5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole)-resistant HCMV strains remained susceptible to 1,6-naphthyridines and 7, 8-dihydroisoquinolines tested in this study, supporting the view that a novel mechanism of action could be involved. Drug combination studies showed a small but significant synergistic antiviral effect between compound B2 and ganciclovir. Cytotoxicity profiling of representative compounds under various cell growth conditions indicated a generally similar cytotoxic effect, relative to ganciclovir, in log-phase growing cells. However, in stationary cells, a relatively higher level of toxicity was observed than that for control compound. Effect of time of drug addition showed that the anti-HCMV activity of compound A1, ganciclovir, and cidofovir was lost at approximately the same time (72 h postinfection), indicating that the compound was affecting events at the early and late stage of virus replication. This interpretation is also supported by reduction of de novo synthesis of pp65 tegument protein and lack of any effect of the compound on viral adsorption. A reduction of the HCMV enhancer-promoter-directed luciferase expression was also observed in a stably transfected cell line when compound A1 was present at relatively high concentrations.
The Immunomodulatory Role of Adjuvants in Vaccines Formulated with the Recombinant Antigens Ov-103 and Ov-RAL-2 against Onchocerca volvulus in Mice (2016)

The mechanism of protective immunity induced by Ov-103 and Ov-RAL-2, with the adjuvants alum, Advax 2 and MF59, appears to be multifactorial with roles for cytokines, chemokines, antibody and specific effector cells. The vaccines developed in this study have the potential of reducing the morbidity associated with onchocerciasis in humans.
Generation and Characterization of Polyclonal Antibodies Specific for Human p110 sEGFR (2002)

The EGFR/ERBB family of receptor tyrosine kinases mediates intracellular signal transduction pathways important in the regulation of cell growth, differentiation, and transformation. We previously have reported the cloning and expression of a 3 kb alternative EGFR transcript which encodes a 110 kDa form of the receptor (p110 sEGFR). This receptor isoform is identical to the extracellular region of the full-length 170 kDa EGFR through amino acid 603; in addition, p110 sEGFR contains 78 unique carboxy-terminal amino acids. Here, we report the generation and characterization of polyclonal antisera specific for the unique carboxy-terminal sequence of p110 sEGFR. Polyclonal antisera were generated by immunizing rabbits with synthetic peptides corresponding to peptides contained within the unique carboxy-terminal sequence of p110 sEGFR. Immunoglobulin fractions from antisera which tested positive for immune reactivity to these peptides by ELISA were affinity-purified by protein G and peptide-based chromatography. This affinity-purified immunoglobulin fraction specifically recognizes p110 sEGFR by ELISA, immunoprecipitation, immunoblot analysis, and immunocytochemical methods. No cross-reactivity with full-length p170 EGFR is observed using any of these detection methods. These new polyclonal antibodies will be useful in determining the expression, localization, and function of p110 sEGFR, and importantly will allow us to distinguish between the expression of this receptor isoform and p170 EGFR.
Inhibition of sphincter of Oddi function by the nitric oxide carrier S-nitroso-N-acetylcysteine in rabbits and humans. (1994)

Nitric oxide (NO) is an inhibitor of gastrointestinal smooth muscle. Model systems of the gut predict the NO will complex with biological thiol (SH) groups, yielding S-nitrosothiols (RS-NO), which may limit the propensity to form mutagenic nitrosamines. The inhibitory effects of NO and its biologically relevant adducts on sphincter of Oddi (SO) motility have been inferred from animal studies; however, their importance in regulating human SO is not known. The objectives of this study were to (a) provide histologic confirmation of nitric oxide synthase (NOS) in human SO; (b) characterize the pharmacology of S-nitroso-N-acetylcysteine (SNAC), an exemplary S-nitrosothiol, on SO motility in a rabbit model; and (c) study the effects of topical SNAC on SO motility in humans. Immunocytochemical and histochemical identification of NOS was performed in human SO. The pharmacologic response of SNAC was defined in isolated rabbit SO using a standard bioassay. Topical SNAC was then applied to the duodenal papilla in patients undergoing endoscopic retrograde cholangiopancreatography (ERCP) and biliary manometry. NOS was localized to nerve fibers and bundles of the SO in rabbits and humans. SNAC inhibited spontaneous motility (frequency and amplitude) as well as acetylcholine-induced elevations in SO basal pressure in the rabbit model. In patients undergoing ERCP and biliary manometry, topical SNAC inhibited SO contraction freqency, basal pressure, and duodenal motility. NOS is localized to neural elements in human SO, implicating a role for NO in regulating SO function. Supporting this concept, SNAC is an inhibitor of SO and duodenal motility when applied topically to humans during ERCP. Our data suggest a novel clinical approach using local NO donors to control gastrointestinal motility and regulate sphincteric function.
High-Throughput Assay and Discovery of Small Molecules that Interrupt Malaria Transmission (2015)

Preventing transmission is an important element of malaria control. However, most of the current available methods to assay for malaria transmission blocking are relatively low throughput and cannot be applied to large chemical libraries. We have developed a high-throughput and cost-effective assay, the Saponin-lysis Sexual Stage Assay (SaLSSA), for identifying small molecules with transmission-blocking capacity. SaLSSA analysis of 13,983 unique compounds uncovered that >90% of well-characterized antimalarials, including endoperoxides and 4-aminoquinolines, as well as compounds active against asexual blood stages, lost most of their killing activity when parasites developed into metabolically quiescent stage V gametocytes. On the other hand, we identified compounds with consistent low nanomolar transmission-blocking activity, some of which showed cross-reactivity against asexual blood and liver stages. The data clearly emphasize substantial physiological differences between sexual and asexual parasites and provide a tool and starting points for the discovery and development of transmission-blocking drugs.
Understanding the limit and potential in emerging perovskite solar cells (2017)

In this contribution we give an overview of the recent development of metal-halide perovskite solar cells. We focus on the mixed electronic-ionic conductivity of this material, which causes hysteresis in the current-voltage curve. We discuss latest results on recombination obtained by electroluminescence measurements. We report on high luminescence yields (1%) and open-circuit voltages larger than 1.2 V, which are exceptional for a material with a bandgap of 1.6 eV and yield to efficiencies >21%. We identify the efficiency limiting processes and propose measures to overcome those.
A potential role for triglyceride as an energy source during bovine oocyte maturation and early embryo development (2006)

The potential role of endogenous triglyceride in bovine oocyte maturation and preimplantation development has been investigated. Bovine immature oocytes were recovered from abattoir-derived ovaries, matured and fertilised in vitro and the zygotes grown to the blastocyst stage in SOFaaBSA. Methyl palmoxirate (MP) blocks the oxidation of fatty acids by inhibiting mitochondrial carnitine palmitoyltransferase A. The development of zygotes exposed to MP during oocyte maturation, and of zygotes exposed to MP during embryo culture has been assessed in terms of oxygen consumption by oocytes and embryos during a 4-6 hr incubation period in the presence of MP and as blastocyst formation and cell number. Immature oocytes exposed to MP during maturation had reduced capacity to form blastocysts after fertilisation; the same effect was apparent, but to a lesser extent, in zygotes exposed to MP during embryo development. Oxygen consumption values of oocytes and blastocysts in the absence of exogenous substrates were similar to those in control medium containing nutrients. MP-inhibited oxygen consumption of immature oocytes, mature oocytes, cleavage stages embryos and blastocysts by 64, 45, 12 and 13%, respectively. The data are consistent with a role for triglyceride as a key energy source during bovine oocyte maturation and potentially, during preimplantation embryo development.
Learning to detect user activity and availability from a variety of sensor data (2004)

Using a networked infrastructure of easily available sensors and context-processing components, we are developing applications for the support of workplace interactions. Notions of activity and availability are learned from labeled sensor data based on a Bayesian approach. The higher-level information on the users is then automatically derived from low-level sensor information in order to facilitate informal ad hoc communications between peer workers in an office environment.
Response-Time Measures as a Means of Exploring Tonal Hierarchies (1988)

We explore the possibility of studying music perception with responsetime measures. Subjects heard either a chord (tonic triad) or scale prime, followed by a single note, and indicated whether the note did or did not belong in the primed key. Overall, the data resemble the tonal hierarchy previously demonstrated with other methods, thus establishing the validity of the response-time measure. In addition, the scale primes superimpose a recency effect on the standard hierarchy, as would be expected from a serially presented stimulus. We discuss what this implies about tonal hierarchies, and the use of response-time measures to study the online processes of music listening. We also report data for nondiatonic tones.
Diode-pumped solid state laser for inertial fusion energy (1994)

The authors evaluate the prospect for development of a diode-pumped solid-state-laser driver in an inertial fusion energy power plant. Using a computer code, they predict that their 1 GWe design will offer electricity at 8.6 cents/kW {center_dot} hr with the laser operating at 8.6% efficiency and the recycled power level at 31%. The results of their initial subscale experimental testbed of a diode-pumped solid state laser are encouraging, demonstrating good efficiencies and robustness.
Prescription for long-lifetime, high-diffraction-efficiency fixed holograms in Fe-doped LiNbO_3 (1997)

A prescription for a material preparation and fixing technique is presented that permits a thermally fixed hologram to be recorded in Fe-doped LiNbO(3) with high diffraction efficiency and a long lifetime. In particular, a hologram with a fixed refractive-index modulation of nearly 10(-3) and a 100-year lifetime at room temperature is predicted.
A targeted functional RNA interference screen uncovers glypican 5 as an entry factor for hepatitis B and D viruses (2015)

These findings advance our understanding of virus cell entry and open new avenues for curative therapies. As glypicans have been shown to play a role in the control of cell division and growth regulation, virus-glypican 5 interactions may also play a role in the pathogenesis of virus-induced liver disease and cancer.
Passivation of InP-based HBTs for high bit rate circuit applications (2002)

We have studied different materials (silicon nitride, silicon oxide, polyimide) for passivating InP-based HBTs, and their influence on the device electrical performances. Polyimide was found to induce the least degradation after passivation. A double heterojunction InP/InGaAs HBT fabrication process, including polyimide passivation and planarization, has been assembled, allowing us to realize high bit-rate circuits, such as a 36 Gb/s 2:1 multiplexer.
Quantum Controlled Cold Scattering Challenges Theory (2022)

Our previous rotationally inelastic cold scattering experiments between state prepared D<sub>2</sub> (<i>v</i> = 2, <i>j</i> = 2, <i>m</i> = 0) and He disagreed with theory, raising serious concerns about either our understanding of the anisotropic potential or the accuracy of the measurement. To further interrogate interactions between molecular hydrogen and atomic helium, we study the Δ<i>j</i> = 1and Δ<i>j</i> = 2 rotational relaxation of HD (<i>v</i> = 2, <i>j</i> = 2, <i>m</i> = 0) by collision with He. The two rotational transitions probe different anisotropic components of the van der Waals potential. Our state resolved scattering study shows that these two transitions are mediated by two different shape resonances <i>l</i> = 1 for Δ<i>j</i> = 1 and <i>l</i> = 2 for Δ<i>j</i> = 2. The strong <i>l</i> = 1 resonance dominates the Δ<i>j</i> = 1 scattering, agreeing with theory. However, the dominance of the weaker <i>l</i> = 2 resonance in the Δ<i>j</i> = 2 transition, which matches our earlier D<sub>2</sub>-He result, contradicts theoretical calculations. The continued contradiction, when we expect one-to-one correspondence between our stereodynamically controlled scattering experiment and theoretical calculations, makes us question the accuracy of the weaker anisotropic part of the H<sub>2</sub>-He interaction potential.
Influence of Phosphoramidites in Copper-Catalyzed Conjugate Borylation Reaction (2012)

Copper(I) has become the preferred metal to catalyze the β-boration of α,β-unsaturated carbonyl compounds, and now we demonstrate that easily accessible monodentate chiral ligands, such as phosphoramidites and phosphites, can be convenient alternative ligands to induce asymmetry in the enantioselective version of this reaction, particularly in the β-boration of α,β-unsaturated imines.
New hydraulic insights into rapid sand filter bed backwashing using the Carman–Kozeny model (2021)

Fluid flow through a bed of solid particles is an important process that occurs in full-scale water treatment operations. The Carman-Kozeny model remains highly popular for estimating the resistance across the bed. It is common practice to use particle shape factors in fixed bed state to match the predicted drag coefficient with experimentally obtained drag coefficients. In fluidised state, however, where the same particles are considered, this particle shape factor is usually simply omitted from the model without providing appropriate reasoning. In this research, it is shown that a shape factor is not a constant particle property but is dependent on the fluid properties as well. This dynamic shape factor for irregularly shaped grains increases from approximately 0.6 to 1.0 in fluidised state. We found that unstable packed beds in moderate up-flow conditions are pseudo-fixed and in a setting state. This results in a decreasing bed voidage and simultaneously in a decreasing drag coefficient, which seems quite contradictory. This can be explained by the collapse of local channels in the bed, leading to a more uniform flow distribution through the bed and improving the available surface for flow-through. Our experimental measurements show that the drag coefficient decreases considerably in the laminar and transition regions. This is most likely caused by particle orientation, realignment and rearrangement in particles' packing position. A thorough hydraulic analysis shows that up-flow filtration in rapid sand filters under backwash conditions causes the particle bed to collapse almost imperceptibly. In addition, an improved expression of the drag coefficient demonstrated that the Carman-Kozeny model constant, however often assumed to be constant, is in fact not constant for increasing flow rates. Furthermore, we propose a new pseudo-3D image analysis for particles with an irregular shape. In this way, we can explain the successful method using optimisation of the extended terminal sub-fluidisation wash (ETSW) filter backwashing procedure, in which turbidity and peaks in the number of particles are reduced with a positive effect on water quality.
Differential regulation of Cav3.2 and Cav2.2 calcium channels by CB1 receptors and cannabidiol (2023)

Our findings reveal differential modulation of Ca<sub>v</sub> 2.2 and Ca<sub>v</sub> 3.2 channels by CB<sub>1</sub> receptors and CBD. This advances our understanding of how different cannabinoids produce analgesia through action at different voltage-gated calcium channels and could influence the development of novel cannabinoid-based therapeutics for treatment of chronic pain.
Generation of gold nanoclusters encapsulated in an MCM-22 zeolite for the aerobic oxidation of cyclohexane (2019)

In this work, we will report the generation of Au clusters in a purely siliceous MCM-22 zeolite. The catalytic properties of these Au clusters have been tested for the selective oxidation of cyclohexane to cyclohexanol and cyclohexanone (KA-oil). The Au clusters encapsulated in the MCM-22 zeolite are highly active and selective for the oxidation of cyclohexane to KA-oil, which is superior to Au nanoparticles on the same support. These results suggest that Au clusters are highly active for the activation of oxygen to produce radical species.
Vaccination Directed against the Human Endogenous Retrovirus-K Envelope Protein Inhibits Tumor Growth in a Murine Model System (2013)

Human endogenous retrovirus (HERV) genomes are chromosomally integrated in all cells of an individual. They are normally transcriptionally silenced and transmitted only vertically. Enhanced expression of HERV-K accompanied by the emergence of anti-HERV-K-directed immune responses has been observed in tumor patients and HIV-infected individuals. As HERV-K is usually not expressed and immunological tolerance development is unlikely, it is an appropriate target for the development of immunotherapies. We generated a recombinant vaccinia virus (MVA-HKenv) expressing the HERV-K envelope glycoprotein (ENV), based on the modified vaccinia virus Ankara (MVA), and established an animal model to test its vaccination efficacy. Murine renal carcinoma cells (Renca) were genetically altered to express E. coli beta-galactosidase (RLZ cells) or the HERV-K ENV gene (RLZ-HKenv cells). Intravenous injection of RLZ-HKenv cells into syngenic BALB/c mice led to the formation of pulmonary metastases, which were detectable by X-gal staining. A single vaccination of tumor-bearing mice with MVA-HKenv drastically reduced the number of pulmonary RLZ-HKenv tumor nodules compared to vaccination with wild-type MVA. Prophylactic vaccination of mice with MVA-HKenv precluded the formation of RLZ-HKenv tumor nodules, whereas wild-type MVA-vaccinated animals succumbed to metastasis. Protection from tumor formation correlated with enhanced HERV-K ENV-specific killing activity of splenocytes. These data demonstrate for the first time that HERV-K ENV is a useful target for vaccine development and might offer new treatment opportunities for diverse types of cancer.