gebhart/patrepeval huggingface dataset:
Granzyme B–Activated p53 Interacts with Bcl-2 To Promote Cytotoxic Lymphocyte–Mediated Apoptosis (2014)

Granzyme B (GzmB) plays a major role in CTLs and NK cell-mediated elimination of virus-infected cells and tumors. Human GzmB preferentially induces target cell apoptosis by cleaving the proapoptotic Bcl-2 family member Bid, which, together with Bax, induces mitochondrial outer membrane permeabilization. We previously showed that GzmB also induces a rapid accumulation of the tumor-suppressor protein p53 within target cells, which seems to be involved in GzmB-induced apoptosis. In this article, we show that GzmB-activated p53 accumulates on target cell mitochondria and interacts with Bcl-2. This interaction prevents Bcl-2 inhibitory effect on both Bax and GzmB-truncated Bid, and promotes GzmB-induced mitochondrial outer membrane permeabilization. Consequently, blocking p53-Bcl-2 interaction decreases GzmB-induced Bax activation, cytochrome c release from mitochondria, and subsequent effector caspases activation leading to a decreased sensitivity of target cells to both GzmB and CTL/NK-mediated cell death. Together, our results define p53 as a new important player in the GzmB apoptotic signaling pathway and in CTL/NK-induced apoptosis.
SLALOM: semiconductor laser amplifier in a loop mirror (1995)

The processing of optical signals in the optical domain is an important issue resulting from the desire to take advantage of the full bandwidth of the optical fiber. In this paper, we present detailed investigations on a device, which utilizes a semiconductor laser amplifier in a loop mirror configuration (SLALOM). Different modes of operation are reported like nonlinear single pulse switching and two-pulse switching at different operation speeds (1-100 Gb/s). Furthermore, a number of applications of the SLALOM in photonic systems, like pulse shaping, decoding, retiming and time-division demultiplexing, are presented. In addition, the SLALOM can be used for an estimate of the linewidth enhancement factor /spl alpha/ and the carrier lifetime /spl tau//sub e/ in an SLA.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
User-Generated services: Policy Management and access control in a cross-domain environment (2015)

The rapid evolution of mobile computing, together with the spread of social networks is increasingly moving the role of users from simple information and services consumers to actual producers. Currently, while most of the critical aspects related to User-Generated Contents (UGC) have been addressed, many issues related to service generation still must be faced and represent the next challenge. In this work, we focus on security issues raised by a particular kind of services: those generated by users. User-Generated Services (UGS) are characterized by a set of features that distinguish them from conventional services. To cope with UGS security problems we introduce three possible policy management models, analyzing benefits and drawbacks of each approach. Finally, we propose a cloud-based solution that enables the composition of multiple UGS and policy models, allowing user's devices to share features and services among them.
Safranal Enhances Non‐Rapid Eye Movement Sleep in Pentobarbital‐Treated Mice (2012)

These findings indicate that safranal enhances NREM sleep in pentobarbital-treated mice. The hypnotic effects of safranal may be related to the activation of the sleep-promoting neurons in the VLPO and the simultaneous inhibition of the wakefulness-promoting neurons in the TMN, suggesting that safranal may be a hypnotic substance.
Time-dependent, irreversible entropy production and geodynamics (2009)

We present an application of entropy production as an abstraction tool for complex processes in geodynamics. Geodynamic theories are generally based on the principle of maximum dissipation being equivalent to the maximum entropy production. This represents a restriction of the second law of thermodynamics to its upper bound. In this paper, starting from the equation of motion, the first law of thermodynamics and decomposition of the entropy into reversible and irreversible terms,(1) we come up with an entropy balance equation in an integral form. We propose that the extrema of this equation give upper and lower bounds that can be used to constrain geodynamics solutions. This procedure represents an extension of the classical limit analysis theory of continuum mechanics, which considers only stress and strain rates. The new approach, however, extends the analysis to temperature-dependent problems where thermal feedbacks can play a significant role. We apply the proposed procedure to a simple convective/conductive heat transfer problem such as in a planetary system. The results show that it is not necessary to have a detailed knowledge of the material parameters inside the planet to derive upper and lower bounds for self-driven heat transfer processes. The analysis can be refined by considering precise dissipation processes such as plasticity and viscous creep.
Distribution Analyzer, a methodology for identifying and clustering outlier conditions from single-cell distributions, and its application to a Nanog reporter RNAi screen (2015)

Using our methodology, we describe each experimental condition by a probability distribution. Measuring distances between probability distributions permits a multivariate rather than univariate readout. Clustering points derived from these distances allows us to obtain greater biological insight than methods based solely on single parameters. We find several outliers from a mouse ESC RNAi screen that we confirm to be pluripotency regulators. Many of these outliers would have been missed by other analysis methods.
Hydrogen Bond Networks in Graphene Oxide Composite Paper: Structure and Mechanical Properties (2010)

A multilayered composite structure formed by a random stacking of graphene oxide (GO) platelets is an attractive candidate for novel applications in nanoelectromechanical systems and paper-like composites. We employ molecular dynamics simulations with reactive force fields to elucidate the structural and mechanical properties of GO paper-like materials. We find that the large-scale properties of these composites are controlled by hydrogen bond networks that involve functional groups on individual GO platelets and water molecules within the interlayer cavities. Water content controls both the extent and collective strength of these interlayer hydrogen bond networks, thereby affecting the interlayer spacing and elastic moduli of the composite. Additionally, the chemical composition of the individual GO platelets also plays a critical role in establishing the mechanical properties of the composite--a higher density of functional groups leads to increased hydrogen bonding and a corresponding increase in stiffness. Our studies suggest the possibility of tuning the properties of GO composites by altering the density of functional groups on individual platelets, the water content, and possibly the functional groups participating in hydrogen bonding with interlayer water molecules.
Operation of individual integrally gated carbon nanotube field emitter cells (2002)

In this work, we examine the operation of individual field emitter cells contained in a field emitter array composed of integrally gated multiwalled carbon nanotube (MWNT)-based field emission cathodes. These devices were found to behave in a manner consistent with a multiple emission site model of Fowler–Nordheim field emission. These results show considerable variation in the operational characteristics of cells contained within the same array and indicate that data obtained from arrays of cells are not necessarily indicative of individual cell performance.
Genetic and molecular risk factors within the newly identified primate‐specific exon of the SAP97/DLG1 gene in the 3q29 schizophrenia‐associated locus (2017)

The synapse-associated protein 97/discs, large homolog 1 of Drosophila (DLG1) gene encodes synaptic scaffold PDZ proteins interacting with ionotropic glutamate receptors including the N-methyl-D-aspartate type glutamate receptor (NMDAR) that is presumed to be hypoactive in brains of patients with schizophrenia. The DLG1 gene resides in the chromosomal position 3q29, the microdeletion of which confers a 40-fold increase in the risk for schizophrenia. In the present study, we performed genetic association analyses for DLG1 gene using a Japanese cohort with 1808 schizophrenia patients and 2170 controls. We detected an association which remained significant after multiple comparison testing between schizophrenia and the single nucleotide polymorphism (SNP) rs3915512 that is located within the newly identified primate-specific exon (exon 3b) of the DLG1 gene and constitutes the exonic splicing enhancer sequence. When stratified by onset age, although it did not survive multiple comparisons, the association was observed in non-early onset schizophrenia, whose onset-age selectivity is consistent with our recent postmortem study demonstrating a decrease in the expression of the DLG1 variant in early-onset schizophrenia. Although the present study did not demonstrate the previously reported association of the SNP rs9843659 by itself, a meta-analysis revealed a significant association between DLG1 gene and schizophrenia. These findings provide a valuable clue for molecular mechanisms on how genetic variations in the primate-specific exon of the gene in the schizophrenia-associated 3q29 locus affect its regulation in the glutamate system and lead to the disease onset around a specific stage of brain development.
Myocardial salvage by T2W-CMR: direct comparison to a non-destructive, high resolution, 3-dimensional ex-vivo assessment of the area at risk simultaneous with infarction (2013)

Background The amount of myocardial salvage is a critical determinant of prognosis in acute myocardial infarction (AMI). T2WCMR in combination with DE-CMR is thought to be a promising method that could presumably measure salvage. However, direct comparisons with the appropriate reference standard are limited. Additionally, even if a pathology reference standard is present, co-registration with in-vivo imaging is problematic. We recently validated a new ex-vivo CMR protocol against the reference standard of microspheres and TTC that provides 3D, non-destructive, high-resolution maps of the AAR simultaneously with infarction[1]. Because this map delineates both the AAR and infarction, salvage can be easily measured in a single image, rather than being calculated from 2 datasets. Additionally, the 3D map allows for direct matching of in-vivo DE to ex-vivo CMR and thereby further minimizing the potential for misregistration from in-vivo DE and more importantly in-vivo T2. Therefore, we sought to compare in-vivo calculated salvage to the directly measured salvage by ex-vivo CMR.
8-Substituted Analogues of 3-(3-Cyclopentyloxy-4-methoxy-benzyl)-8-isopropyl- adenine: Highly Potent and Selective PDE4 Inhibitors (2005)

3-(3-Cyclopentyloxy-4-methoxy-benzyl)-8-isopropyl-adenine V11294 (1) has been identified as a lead structure, which selectively inhibits human lung PDE4 (436 nM) and is also active in a number of in vitro and in vivo models of inflammation. Here we describe the synthesis and pharmacology of a series of highly potent 8-[(benzyloxy)methyl]-substituted analogues, with potencies in the range 10-300 nM. In addition, several compounds showed interesting PDE4 subtype specificities, for example, the 3-thienyl derivative 5v, which showed 6-10 nM potency at PDE4B, D3, and D5 and a 20- to 200-fold selectivity over A and D2, respectively.
Unraveling the Origin of Operational Instability of Quantum Dot Based Light-Emitting Diodes (2018)

We investigate the operational instability of quantum dot (QD)-based light-emitting diodes (QLEDs). Spectroscopic analysis on the QD emissive layer within devices in chorus with the optoelectronic and electrical characteristics of devices discloses that the device efficiency of QLEDs under operation is indeed deteriorated by two main mechanisms. The first is the luminance efficiency drop of the QD emissive layer in the running devices owing to the accumulation of excess electrons in the QDs, which escalates the possibility of nonradiative Auger recombination processes in the QDs. The other is the electron leakage toward hole transport layers (HTLs) that accompanies irreversible physical damage to the HTL by creating nonradiative recombination centers. These processes are distinguishable in terms of the time scale and the reversibility, but both stem from a single origin, the discrepancy between electron versus hole injection rates into QDs. Based on experimental and calculation results, we propose mechanistic models for the operation of QLEDs in individual quantum dot levels and their degradation during operation and offer rational guidelines that promise the realization of high-performance QLEDs with proven operational stability.
Craniofacial Shortening by Contraction Osteogenesis: An Experimental Model (2000)

Application of gradual external forces to correct craniofacial deformities challenges many procedures in conventional craniomaxillofacial surgery. Distraction osteogenesis is replacing traditional osteotomies for correction of patients with craniomaxillofacial deficiencies. However, the reverse concept, contraction osteogenesis, has yet to be established for patients with craniomaxillofacial excesses. The purpose of this investigation is to demonstrate the contraction osteogenesis phenomenon applied in a controlled animal model during the craniofacial growth period. Twenty-six 26-day-old rabbits were assigned to one of four groups: 0, control; 1, pin control (pin insertion); 2, no contraction (pins and contraction device application, without active contraction); and 3, contraction (pin insertion, contraction device application, and active contraction). An external fixator was placed across the incisive-maxillary suture, and the effects after 4.5 weeks of contraction at a rate of 0.5 mm twice a week were compared with control groups. The results were assessed by craniometric and cephalometric measurements and by histologic examination. Gross alterations were evident in the contraction group, characterized by midface anteroposterior shortening, maxillary regression, snout deviation, and anterior crossbite. Histologic examination of the contraction group demonstrated a significant increase in osteoblastic activity. Contraction osteogenesis is a new treatment concept in craniofacial development and may offer therapeutic opportunities for shortening skeletal structures without the need of osteotomies, thus taking advantage of the potential of craniofacial growth and remodeling.
Abstract 1703: The HDAC6 inhibitor Nexturastat A improves in vivo PD-1 immune blockade (2018)

Abstract The role of HDACs in cell biology, initially limited to their effects upon histones, now encompasses more complex regulatory functions that vary with their tissue expression, cellular compartmentalization and stage of cellular differentiation. Several recent studies have shown that selective HDAC inhibitors (HDACi) are able to impair in vivo tumor growth. Therefore, there is an emerging interest in the understanding of the molecular mechanisms mediating these anti-tumor properties. In this context, a number of recent publications have demonstrated that the selective inhibition of specific HDACs enhances tumor immunogenicity in a wide variety of tumors, thereby preventing tumor escape and improving immune surveillance. Our group has focused on HDAC6, and shown that both the genetic abrogation and pharmacological inhibition of this HDAC modulates the expression of a variety of immune-regulatory proteins in the tumor microenvironment, including PD-L1, PD-L2, MHC class I, B7-H4 and TRAIL-R1. In particular, we have previously demonstrated that both pharmacological inhibition and/or genetic abrogation of HDAC6 plays a critical role in the immune check point blockade by down-regulating the expression of PD-L1. Moreover, we have also shown that in vivo inhibition of HDAC6 reduces tumor growth in B16 and SM1 murine melanoma models within syngeneic immunocompetent hosts. Additionally, we have found that the combination of the HDAC6i Nexturastat A (NextA) and checkpoint PD-1 immune blockade therapy results in an important improvement in anti-tumor immune responses as evidenced by the reduction of tumor growth when compared to treatment with individual stand-alone agents. Moreover, our data has shown an increase in CD8+, NK+ cell infiltration of tumors treated with the combination therapy versus either standalone treatment. Further analysis of in vivo immune tumor infiltration evidenced that the treatment with NextA importantly diminished the macrophage M2 phenotype in the tumor microenvironment. An outcome that was also observed in combination with PD-1 immune blockade. Lastly, we have found that the anti-PD-1/NextA in vivo combination therapy reduces the expression of PD-L1, PD-L2 and other negative check-point pathways. However, the HDAC6 inhibition has a minimal effect over the expression of their respective counterparts in T cells (i.e. PD-1). Overall, we have found that HDAC6i could be used as a potential adjuvant in ongoing therapeutic options involving immune check-point blockade. Citation Format: Tessa Knox, Eva Sahakian, Debarati Banik, Melissa Hadley, Erica Palmer, Jennifer Kim, John Powers, Sarthak Shah, Alexa Lowe, Javier Pinilla, Eduardo Sotomayor, Norman Lee, Alan Kozikowski, Alejandro V. Villagra. The HDAC6 inhibitor Nexturastat A improves in vivo PD-1 immune blockade [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 1703.
Measurement of Dipolar Couplings in a Uniformly 13C,15N-Labeled Membrane Protein:  Distances between the Schiff Base and Aspartic Acids in the Active Site of Bacteriorhodopsin (2001)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTMeasurement of Dipolar Couplings in a Uniformly 13C,15N-Labeled Membrane Protein: Distances between the Schiff Base and Aspartic Acids in the Active Site of BacteriorhodopsinChristopher P. Jaroniec, Jonathan C. Lansing, Brett A. Tounge, Marina Belenky, Judith Herzfeld, and Robert G. GriffinView Author Information Department of Chemistry and Francis Bitter Magnet Laboratory Massachusetts Institute of Technology Cambridge, Massachusetts 02139 Department of Chemistry, Brandeis University Waltham, Massachusetts 02254 Cite this: J. Am. Chem. Soc. 2001, 123, 51, 12929–12930Publication Date (Web):November 28, 2001Publication History Received23 August 2001Published online28 November 2001Published inissue 1 December 2001https://pubs.acs.org/doi/10.1021/ja016923rhttps://doi.org/10.1021/ja016923rrapid-communicationACS PublicationsCopyright © 2001 American Chemical SocietyRequest reuse permissionsArticle Views406Altmetric-Citations34LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Coupling reactions,Monomers,Nitrogen,Peptides and proteins,Physical and chemical processes Get e-Alerts
Blockchains for Business Process Management - Challenges and Opportunities (2017)

Blockchain technology promises a sizable potential for executing inter-organizational business processes without requiring a central party serving as a single point of trust (and failure). This paper analyzes its impact on business process management (BPM). We structure the discussion using two BPM frameworks, namely the six BPM core capabilities and the BPM lifecycle. This paper provides research directions for investigating the application of blockchain technology to BPM.
A Novel Initiation System for the Cationic Polymerization of Glycidyl Phenyl Ether:  Triphenylcyclopropenium Tetrakis(pentafluorophenyl)gallate/Cyclohexanone (2001)

ADVERTISEMENT RETURN TO ISSUECommunication to the...Communication to the EditorNEXTA Novel Initiation System for the Cationic Polymerization of Glycidyl Phenyl Ether: Triphenylcyclopropenium Tetrakis(pentafluorophenyl)gallate/CyclohexanoneHuiying Li, Kangtai Ren, Wenqin Zhang, John H. Malpert, and Douglas C. NeckersView Author Information Center for Photochemical Sciences, Bowling Green State University, Bowling Green, Ohio 43403, and Spectra Group Limited, Inc., 1722 Indian Wood Circle, Suite H, Maumee, Ohio 43537 Cite this: Macromolecules 2001, 34, 7, 2019–2021Publication Date (Web):February 22, 2001Publication History Received7 November 2000Revised16 January 2001Published online22 February 2001Published inissue 1 March 2001https://pubs.acs.org/doi/10.1021/ma001912ohttps://doi.org/10.1021/ma001912orapid-communicationACS PublicationsCopyright © 2001 American Chemical SocietyRequest reuse permissionsArticle Views191Altmetric-Citations9LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Anions,Cationic polymerization,Cations,Ethers,Polymerization Get e-Alerts
Supplementary Table 2 from Nuclear–Cytoplasmic Transport Is a Therapeutic Target in Myelofibrosis (2023)

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Imaging individual neurons in the retinal ganglion cell layer of the living eye (2017)

Significance Retinal ganglion cells are the primary output neurons of the retina that process visual information and transmit it to the brain. We developed a method to reveal these cells in the living eye that does not require the fluorescent labels or high light levels that characterize more invasive methods. The death of these cells causes vision loss in glaucoma, the second leading cause of blindness worldwide. The ability to image these cells in the living eye could accelerate our understanding of their role in normal vision and provide a diagnostic tool for evaluating new therapies for retinal disease.