gebhart/patrepeval huggingface dataset:
Characterization and molecular cloning of a heterodimeric Î²-galactosidase from the probiotic strainLactobacillus acidophilusR22 (2007)

Beta-galactosidase from the probiotic strain Lactobacillus acidophilus R22 was purified to apparent homogeneity by ammonium sulphate fractionation, hydrophobic interaction, and affinity chromatography. The enzyme is a heterodimer consisting of two subunits of 35 and 72 kDa, as determined by gel electrophoresis. The optimum temperature of beta-galactosidase activity was 55 degrees C (10-min assay) and the range of pH 6.5-8, respectively, for both o-nitrophenyl-beta-D-galactopyranoside (oNPG) and lactose hydrolysis. The Km and Vmax values for lactose and oNPG were 4.04+/-0.26 mM, 28.8+/-0.2 micromol D-glucose released per min per mg protein, and 0.73+/-0.07 mM, 361+/-12 micromol o-nitrophenol released per min per mg protein, respectively. The enzyme was inhibited by high concentrations of oNPG with Ki,s=31.7+/-3.5 mM. The enzyme showed no specific requirements for metal ions, with the exception of Mg2+, which enhanced both activity and stability. The genes encoding this heterodimeric enzyme, lacL and lacM, were cloned, and compared with other beta-galactosidases from lactobacilli. Beta-galactosidase from L. acidophilus was used for the synthesis of prebiotic galacto-oligosaccharides (GOS) from lactose, with the maximum GOS yield of 38.5% of total sugars at about 75% lactose conversion.
Changes in Muscle Cell Metabolism and Mechanotransduction Are Associated with Myopathic Phenotype in a Mouse Model of Collagen VI Deficiency (2013)

This study identifies metabolic and protein phenotypic alterations in gastrocnemius, tibialis anterior and diaphragm muscles of Col6a1(-/-) mice, a model of human collagen VI myopathies. All three muscles of Col6a1(-/-) mice show some common changes in proteins involved in metabolism, resulting in decreased glycolysis and in changes of the TCA cycle fluxes. These changes lead to a different fate of α-ketoglutarate, with production of anabolic substrates in gastrocnemius and tibialis anterior, and with lipotoxicity in diaphragm. The metabolic changes are associated with changes of proteins involved in mechanotransduction at the myotendineous junction/costameric/sarcomeric level (TN-C, FAK, ROCK1, troponin I fast) and in energy metabolism (aldolase, enolase 3, triose phosphate isomerase, creatine kinase, adenylate kinase 1, parvalbumin, IDH1 and FASN). Together, these change may explain Ca(2+) deregulation, impaired force development, increased muscle-relaxation-time and fiber damage found in the mouse model as well as in patients. The severity of these changes differs in the three muscles (gastrocnemius<tibialis anterior<diaphragm) and correlates to the mass-to-tendon (myotendineous junction) ratio and to muscle morphology.
Demonstrating preemptive reaction (2019)

In this demonstration we enable preemptive force-feedback systems to speed up human reaction time without fully compromising the user's sense of agency. Typically these haptic systems speed up human reaction time by means of electrical muscle stimulation (EMS) or mechanical actuation (exoskeletons), which unfortunately, completely remove the users sense of agency. We address this by actuating the user's body, using EMS, within a particular time window (160 ms after visual stimulus), which we found to speed up reaction time by 80 ms, while retaining a sense of agency. Here, we demonstrate this at the example of two applications: (1) taking a picture of a high-speed moving object in mid-flight, or (2) hit baseball with a toy gun.
Fundamental studies on cationic polymerizations: Molecular weights and molecular weight distributions of polyisobutylenes produced by γ‐irradiation (free ions) and chemical catalysis (ion pairs) (1971)

Abstract The molecular weights (M̄ n , M̄ v , and M̄ w ) and molecular weight distributions of polyisobutylenes synthesized by γ‐irradiation in the range 29 to −78°C have been determined. The plots of log M̄ n , log M̄ v , and log M̄ w versus 1/ T are linear and parallel ( E M̄ = −6.3 6 ± 0.5), and M̄ w /M̄ n ≈ 3.0 ± 0.5 over this temperature range. The viscosity‐average molecular weights of polyisobutylenes obtained by γ‐irradiation were compared with those of polymers prepared by BF 3 , EtAlCl 2 , and AlCl 3 . It is found that, at the same polymerization temperature, the M̄ v of radiation‐induced polyisobutylenes, which propagate by free carbonium ions, is significantly higher than those synthesized by the Lewis acids, which probably propagate via ion pairs. The implications of these findings are discussed, and it is concluded that the counterion in the proximity of the growing cation impedes propagation to a greater extent than it does the competing process of chain transfer to monomer.
Core pumped Erbium fiber nanosecond pulse amplifier generating 360 kW peak power with M2 < 1.1 at 1545 nm wavelength (2007)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text J. Jasapara, M. Andrejco, D. DiGiovanni, C. Headley, and A. D. Yablon, "Core pumped Erbium fiber nanosecond pulse amplifier generating 360 kW peak power with M2 < 1.1 at 1545 nm wavelength," in Advanced Solid-State Photonics, OSA Technical Digest Series (CD) (Optica Publishing Group, 2007), paper WE1. Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
Val/Val genotype of brain-derived neurotrophic factor (BDNF) Val66Met polymorphism is associated with a better response to OROS-MPH in Korean ADHD children (2011)

Research on psychostimulants, analysis of animal models and genetic association studies all suggest that the brain-derived neurotrophic factor gene (BDNF) may be a good candidate for pharmacogenetic studies of attention deficit hyperactivity disorder (ADHD). Yet to date there have been no pharmacogenetic studies of BDNF in ADHD. A total of 102 drug-naive ADHD children (8.7±2.1 yr) were treated with osmotic release oral system-methylphenidate (OROS-MPH) for 12 wk, and four kinds of response criteria were applied, based first, on a combined threshold of the ADHD Rating Scale - IV (ARS) and the Clinical Global Impression - Improvement scale (CGI-I); second, on scores of 1 or 2 vs. 3-7 on the CGI - Severity scale; third, on a >50% reduction in ARS scores; and fourth, on satisfaction of all of the aforementioned criteria. The Val⁶⁶Met polymorphism of BDNF and six single nucleotide polymorphisms from the SLC6A2, ADRA2A and NTF-3 genes were tested for association with each criterion. Relative to other genotypes, homozygosity for the Val allele of the BDNF Val⁶⁶Met polymorphism was associated with a greater relative frequency of good response under all four response criteria (after controlling for baseline ARS score, age, gender, final dose (mg/kg) of OROS-MPH at 12 wk, and level of academic functioning). This association was significant at the uncorrected level for the first and third response criteria (p=0.013 and p=0.018, respectively) and significant at a Bonferroni-corrected level for the second and fourth response criteria (p=0.0002, p=0.0003, respectively). Our findings support an association between homozygosity for the Val allele of BDNF and better response to OROS-MPH in Korean ADHD children as assessed by four different response criteria.
Structural Insights into the Quadruplex–Duplex 3′ Interface Formed from a Telomeric Repeat: A Potential Molecular Target (2016)

We report here on an X-ray crystallographic and molecular modeling investigation into the complex 3' interface formed between putative parallel stranded G-quadruplexes and a duplex DNA sequence constructed from the human telomeric repeat sequence TTAGGG. Our crystallographic approach provides a detailed snapshot of a telomeric 3' quadruplex-duplex junction: a junction that appears to have the potential to form a unique molecular target for small molecule binding and interference with telomere-related functions. This unique target is particularly relevant as current high-affinity compounds that bind putative G-quadruplex forming sequences only rarely have a high degree of selectivity for a particular quadruplex. Here DNA junctions were assembled using different putative quadruplex-forming scaffolds linked at the 3' end to a telomeric duplex sequence and annealed to a complementary strand. We successfully generated a series of G-quadruplex-duplex containing crystals, both alone and in the presence of ligands. The structures demonstrate the formation of a parallel folded G-quadruplex and a B-form duplex DNA stacked coaxially. Most strikingly, structural data reveals the consistent formation of a TAT triad platform between the two motifs. This triad allows for a continuous stack of bases to link the quadruplex motif with the duplex region. For these crystal structures formed in the absence of ligands, the TAT triad interface occludes ligand binding at the 3' quadruplex-duplex interface, in agreement with in silico docking predictions. However, with the rearrangement of a single nucleotide, a stable pocket can be produced, thus providing an opportunity for the binding of selective molecules at the interface.
A case of elderly patient with carcinoma of lower gingiva which dramatically responded to oral administration of UFT. (1997)

症例は左側下顎臼歯部歯肉癌の80歳の女性である。病理組織学的診断は扁平上皮癌であった。UFTの経口単独投与を行ったところ, 腫瘤は徐々に消失し, 投与開始3週間後には臨床的にも組織学的にも腫瘍の消失がみられた。本症例のメタロチオネイン (MT) およびPCNAの発現について免疫組織化学的に検討をした。MTの発現は癌胞巣の周辺部に認められ, その陽性率は約18%と非常に低い値を示した。また, MTの分布様式はPCNAの分布様式と非常に似た分布を示していた。
Cobalt(III) complexes of the macrocycle meso-2,12-dimethyl-3,7,11,17-tetraazabicyclo[11,3,1]heptadeca-1(17),13,15-triene (1969)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCobalt(III) complexes of the macrocycle meso-2,12-dimethyl-3,7,11,17-tetraazabicyclo[11,3,1]heptadeca-1(17),13,15-trieneEiichiro Ochiai and Daryle H. BuschCite this: Inorg. Chem. 1969, 8, 7, 1474–1486Publication Date (Print):July 1, 1969Publication History Published online1 May 2002Published inissue 1 July 1969https://pubs.acs.org/doi/10.1021/ic50077a021https://doi.org/10.1021/ic50077a021research-articleACS PublicationsRequest reuse permissionsArticle Views87Altmetric-Citations16LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Integration and Response of Organic Electronics with Aqueous Microfluidics (2002)

We have successfully integrated microfluid flow channels, defined by surface energy differences, with thin-film organic field-effect transistors. The device performance is stable for specific semiconductors with water flowing over the channel region and the contacts protected by hydrophobic surface films, indicating that organic semiconductors are not necessarily degraded by water even when carrying current. In addition, a substantial response from dilute organic solutes, as low as 1 ppm in an aqueous environment, can be observed, with some degree of semiconductor−solute specificity.
A phase I study of combined trabectedin and pegylated liposomal doxorubicin therapy for advanced relapsed ovarian cancer (2021)

JapicCTI-163164.
OPTIMAL FORCING FREQUENCY FOR ENHANCEMENT OF NEAR-WALL VORTICAL STRUCTURE IN A TURBULENT BOUNDARY LAYER (2005)

Effects of localized periodic blowing on a turbulent boundary layer are investigated using direct numerical simulation. The time-periodic blowing through a spanwise slot is given by varying the wall-normal velocity in a cyclic manner from 0 to 2A+. The time-periodic blowing frequency is given in a range 0≤f+≤0.08 at a fixed blowing amplitude of A+=0.5. Simulations of spatially-evolving turbulent boundary layer are carried out at the Reynolds numbers of 300 and 670 based on the momentum thickness and free-stream velocity. The effects of the blowing frequency are scrutinized by examining the phase- or time-averaged turbulent statistics. A most effective blowing frequency is obtained at f+=0.035 for both Reynolds numbers, where the maximum increases of Reynolds shear stress, streamwise vorticity fluctuations and energy redistribution are made. Reynolds stress budget shows that the effective blowing frequency most enhances the pressure-strain term, which is closely related with the energy redistribution. The analysis of the phase-averaged stretching and tilting terms reveals that the stretching term is significantly enhanced in the 'downward' motion which is induced by the spanwise vortical motion.
Математическое моделирование режимов работы высоконапорного многоступенчатого центробежного компрессора (2016)

Iterative computations in mathematical models are a major method of solving engineering problems of compressor equipment operation (selection of compressor type and capacity, optimization of operation modes and others). Gas dynamic characteristics (GDC) are the basis of a compressor mathematical modeling. The reduced characteristic method is widely used in the centrifugal compressor GDC modeling. The method allows a precise description of GDC for low pressure compressors, but in case of a high pressure it results in high errors of determining the ratio ofpressures and polytropical efficiency compared to the actual data. The need of high pressure compressor application at some of Gazprom facilities has been recently increasing, which requires a specification of modeling methods. The present article focuses on causes and quantitative assessment of the errors, and proposes a technique of improving the precision of centrifugal compressor GDC description for solving engineering problems of designing and operation of compression systems.
Thiol-reactive Derivatives of the Solvatochromic 4-N,N-Dimethylamino-1,8-naphthalimide Fluorophore: A Highly Sensitive Toolset for the Detection of Biomolecular Interactions (2009)

The solvatochromic fluorophore 4-N,N-dimethylamino-1,8-naphthalimide (4-DMN) possesses extremely sensitive emission properties due largely to the low intrinsic fluorescence it exhibits in polar protic solvents such as water. This makes it well suited as a probe for the detection of a wide range of biomolecular interactions. Herein we report the development and evaluation of a new series of thiol-reactive agents derived from this fluorophore. The members of this series vary according to linker type and the electrophilic group required for the labeling of proteins and other biologically relevant molecules. Using the calcium-binding protein calmodulin as a model system, we compare the performance of the 4-DMN derivatives to that of several commercially available solvatochromic fluorophores identifying many key factors important to the successful application of such tools. This study also demonstrates the power of this new series of labeling agents by yielding a fluorescent calmodulin construct capable of producing a greater than 100-fold increase in emission intensity upon binding to calcium.
Design of curved waveguides: the matched bend (2003)

A new criterion for the optimum design of curved dielectric waveguides is proposed. The bends designed according to this model are named matched bends. In the matched bend, the suitable choice of both bending radius and bending angle reduces the total losses of the bend and avoids the leaky-mode excitation at the end of the bend. For a given angle, a discrete number of bending radii that satisfy the matched bend criterion can be analytically determined. With respect to the lateral offset, matched bends are more robust to both fabrication tolerances and wavelength and can be realized in every technology. The reduction of the leaky-mode excitation at the output of the bend is a fundamental property when two or more components are cascaded. Ghost images in the spectral response, cross talk, and asymmetries of the transfer function are successfully reduced. Some examples that use buried, rib, ridge, and diffused waveguides are presented and discussed.
JCSP Agents-Based Service Discovery for Pervasive Computing (2009)

Device and service discovery is a very important topic when considering pervasive environments. The discovery mechanism is required to work in networks with dynamic topology and on limited software, and be able to accept different device descriptions. This paper presents a service and device discovery mechanism using JCSP agents and the JCSP network package jcsp.net2.
FHIT Suppresses Epithelial-Mesenchymal Transition (EMT) and Metastasis in Lung Cancer through Modulation of MicroRNAs (2014)

Metastasis is the principal cause of cancer death and occurs through multiple, complex processes that involve the concerted action of many genes. A number of studies have indicated that the Fragile Histidine Triad (FHIT) gene product, FHIT, functions as a tumor suppressor in a variety of common human cancers. Although there are suggestions of a role for FHIT loss in progression of various cancers, a role for such loss in metastasis has not been defined. Here, via in vivo and in vitro assays, we reveal that the enforced expression of FHIT significantly suppresses metastasis, accompanied by inhibition of the epithelial-mesenchymal transition (EMT), a process involved in metastasis through coordinate modulation of EMT-related genes. Specifically, miR-30c, a FHIT-upregulated microRNA, contributes to FHIT function in suppression of EMT and metastasis by directly targeting metastasis genes Metadherin (MTDH), High-mobility group AT-hook 2 (HMGA2), and the mesenchymal markers, Vimentin (VIM) and Fibronectin (FN1), in human lung cancer. Finally, we demonstrate that the expression pattern of FHIT and miR-30c is inversely correlated with that of MTDH and HMGA2 in normal tissue, non-metastatic and metastatic tumors, serving as a potential biomarker for metastasis in lung cancer.
A Clinical Study of Electroacupuncture and Auricular Acupuncture for Abdominal Pain Relief in Patients with Pancreatitis: A Pilot Study (2017)

Objectives : The purpose of this study is to evaluate the feasibility of further acupuncture research as an effective alternative and safe treatment for abdominal pain control in patients with pancreatitis. Methods and Results : This study is an open-label, assessment-blind, parallel designed pilot clinical trial. Thirty participants will be assigned to the acupuncture group(n=15) and usual care group(n=15). All patients will receive the conventional standard-of-care(SOC) therapy, but only the experimental group will receive acupuncture therapy six times a week, and the duration of acupuncture therapy will be held up to 12 weeks or until the pains are to be resolved. For the conventional SOC therapy, painkiller will be given. In treatment group, the subjects will receive the identical SOC therapy in combination with electroacupuncture therapy on twelve acupuncture points(LI4, PC6, SP6, GB39, ST36, ST37), and auricular acupuncture therapy on five auricular acupuncture points(Sympathetic, Shen Men, Abdomen, Pancreas gall, and Spleen). The primary outcome will be measured using the visual analogue scale(VAS), and the secondary outcome will be measured using the painkiller demand, quality of life index and severity of pancreatitis by abdominal computed tomography(CT). Assessments will be made at baseline and at week 1, 4, 8 and 12. Results of abdominal CT will be evaluated at baseline and at week 12. Conclusions : The result of this trial will provide a basis for the effectiveness and safety of acupuncture treatment for abdominal pain in patients with pancreatitis.
Beyond Omega: next-generation miniature infrared camera (2005)

Three years ago, Indigo Systems launched its Omega camera line, which to this day remains one of the world's smallest, lightest, lowest powered infrared cameras. The concept of a miniature thermal imager has proven very successful, and thousands of cores have been employed in a number of portable applications, including firefighting, unmanned vehicles, and handheld imagers. A common thread to these high-volume markets is their elasticity-lowering cost substantially enhances demand. Hence the motivation behind Indigo's newest miniature camera, Photon. Photon is a product family of small and mid-format sensor engines (160x128, 320x128, 320x256) specifically optimized for low cost and high volume. While it shares many of Omega's positive benefits, including remarkably small size, weight, and power, several aspects of the design contribute to it being more affordable than its fore-runner even with four times as many pixels. This paper compares the Photon design to the Omega with particular focus on those aspects affecting manufacturability and cost.
Imaging Motor Neuron Disease: Lessons from Single Subject Analyses (P4.097) (2016)

Objective: Discuss important imaging concepts, patterns, and pitfalls in ALS Background:Through a collection of interesting patients, we share our growing experience imaging motor neuron disease at the single subject level. We revisit longitudinal follow-up scans for a man with ALS exhibiting left arm weakness, addressing some corrections and improvements in DTI post-processing. We examine DTI and cortical thickness in a patient with dense right arm hemiplegia, looking for clinical correlation. We also examine SWI patterns in two patients. Methods:All patients with MND had 3D T1MPRAGE and b1000 DTI. Some patients had SWI and some had b3000 DTI dataset treated as a qBall scheme. Freesurfer was used to estimate average thickness of primary motor cortex for all patients. Tailored deterministic tractography of the corticospinal tracts was performed for all patients by a CAQ neuroradiologist. Automated probablistic tractography was also performed with TRACULA for all patients. Results:While subtle, right versus left differences in CST tract statistics (FA, mean diffusivity, and radial diffusivity) were observed in cases of asymmetric weakness. Crossing fibers may precipitate erroneous streamlines and alter subsequent statistics; b3000 DTI treated as qBall for spherical harmonic delivered a different CST morphology than b1000 DTI. Average primary motor cortical thickness was diminished (<2.5mm) in all cases of symptomatic motor neuron disease. Conclusions: These cases illustrate pertinent issues concerning DTI processing, more complex diffusion models, automated probabilistic and deterministic tractography, potential role of cortical thickness, and the utility of SWI to investigate iron deposition. Carefully processed CST diffusion data and tract statistics, even at the single subject level, may indicate a more problematic tract and might have potential to predict laterality of symptoms; further study is needed as magnitude of difference is quite small. Recognizing errors in CST tractography that might impact tract values is crucial.
Delocalization of localized modes in nonlinear disordered waveguide lattices (2008)

We present direct experimental measurements of localized eigenmodes in disordered one-dimensional waveguide arrays. In the nonlinear regime we observe delocalization of localized states, exhibiting different features in the limits of weak and strong disorder. (C) 2008 Optical Society of America