gebhart/patrepeval huggingface dataset:
Quantification of the C3d Split Products of Human Complement by a Sensitive Enzyme Linked Immunosorbent Assay (1985)

A double-antibody biotin-avidin enzyme-linked immunosorbent assay (ELISA) for quantification of the C3d split products is described. Polyethylene glycol 6000 was used to precipitate large C3 molecules, and the C3d-containing supernatant was used in the assay. C3d was measured in ethylenediaminetetraacetic acid plasma from 40 healthy blood donors, and the normal range was defined. Twenty-two patients were tested, and 12 of these had increased levels of C3d. No correlation was observed between total C3 and C3d in these patients. There was a close correlation between C3d measured by this method and by the double-decker rocket immunoelectrophoresis method. The C3d ELISA method is very sensitive, easy to perform, and time-saving and economical compared with most C3d methods already described. A procedure to define the lower detection limit and examine the reliability of an ELISA method in general is discussed.
Phosphoinositide 3-kinase α inhibitors: a patent review (2013)

Features of the development of PI3K inhibitors to date have been the plasticity of the enzymes, which possess binding sites for a bewildering number of small molecule scaffolds, and the need to determine the optimal patterns of selectivity between both the PI3K isoforms and the related downstream serine/threonine kinase mammalian target of rapamycin (mTOR) for therapeutic effect. Both themes are apparent in the recent patents reviewed here, with a wide variety of drug types, including variations on existing scaffolds and completely new ones, being evident. While many of these are dual PI3K/mTOR inhibitors, the PI3Kα-selective pyrido[2,3-b]pyrazine heterocycles reported by Intellikine and the (thiazolyl)pyrrolidinecarboxamides of Novartis are of particular interest.
Analysis of mRNA Populations from Single Live and Fixed Cells of the Central Nervous System (1997)

This unit presents a method for the amplification of poly(A)(+) mRNA extracted from the cytoplasm of a single cell. After cDNA is synthesized from the mRNA, it is made double stranded, denatured, and reverse transcribed to yield antisense RNA (aRNA). Another round of amplification results in a relatively large amount of aRNAs in essentially the same proportion as in the starting mRNA population. RNA amplification protocols can be used for many purposes, including generation of disease expression profiles, making of cDNA libraries, and generation of diagnostics and therapeutics for disease. An alternate protocol is used to amplify RNAs from single neurons in fixed tissue specimens. Support protocols gives instructions for reverse northern analysis, which allows analysis of the presence or absence and relative levels of mRNA expression in selected cells, and a convenient method to assess the RNA content in fixed tissue sections using the fluorescent dye acridine orange (which binds single-stranded nucleic acids).
Review of 5G capabilities for smart manufacturing (2021)

5G has been defined to address new use cases beyond consumer-focused mobile broadband services. In particular industrial use cases, for example in smart manufacturing, have been addressed in the 5G standardization, so that 5G can support industrial IoT services and wireless industrial networking. To this end, 5G needs to integrate with the industrial network based on Ethernet and TSN. 5G can create new opportunities for smart manufacturing by enabling flexibility and increasing the automation in the production. This paper provides an overview of the use cases and requirements for smart manufacturing that can be addressed with 5G and which are validated in three industrial 5G trial systems. The capabilities of 5G are described for providing non-public networks that support industrial LAN services based on Ethernet and TSN. The 5G radio access network functionality is described and discussed for practical deployments. The paper provides an overview of the current state-of-the-art of using 5G for smart manufacturing.
Macromolecular backbone rearrangements: a new approach to the synthesis of N-phenyl-substituted polyurethanes (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMacromolecular backbone rearrangements: a new approach to the synthesis of N-phenyl-substituted polyurethanesChun Li and Joachim KohnCite this: Macromolecules 1991, 24, 9, 2302–2308Publication Date (Print):April 29, 1991Publication History Published online1 May 2002Published inissue 29 April 1991https://pubs.acs.org/doi/10.1021/ma00009a028https://doi.org/10.1021/ma00009a028research-articleACS PublicationsRequest reuse permissionsArticle Views140Altmetric-Citations5LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Characterization of broadly neutralizing antibody responses to HIV-1 in a cohort of long term non-progressors (2018)

LTNPs, both viremic and elite controllers, showed broad humoral immune responses against HIV-1, including activity against many major epitopes involved in bNAbs-mediated protection.
A novel leptospiral protein increases ICAM-1 and E-selectin expression in human umbilical vein endothelial cells (2007)

It has been reported previously that activation of vascular endothelium by outer membrane proteins of the spirochetes Borrelia sp. and Treponema sp. resulted in enhanced expression of endothelial cell adhesion molecules. To investigate the role of leptospiral proteins in this process, a predicted lipoprotein encoded by the gene LIC10365 was selected, which belongs to a paralogous family that presents a domain of unknown function, DUF1565. The LIC10365 gene was cloned and the protein expressed in Escherichia coli C43 (DE3) strain using the vector pAE. The recombinant protein tagged with N-terminal hexahistidine was purified by metal-charged chromatography and was used to assess its ability to activate cultured human umbilical vein endothelial cells. The rLIC10365 activated endothelium in such a manner that E-selectin and intercellular adhesion molecule 1 (ICAM-1) became upregulated in a dose-dependent fashion. The LIC10365-encoded protein was identified in vivo in the renal tubules of animal during experimental infection with Leptospira interrogans. Collectively, these results implicate the LIC10365-coding protein of L. interrogans as a potential effector molecule in the promotion of a host inflammatory response. This is the first report of a leptospiral protein capable of up-regulating the expression of endothelial cell adhesion molecules ICAM-1 and E-selectin.
Overview of Materials Processing and Properties of Lanthanum-Based High-k Dielectrics (2007)

Lanthanum-based high-K dielectrics remain attractive for gate dielectric applications, in the forms of either lanthanum oxide, lanthanum silicate, or as a lanthanum addition to other dielectrics. Lanthanum oxide and silicate MIS devices have been reported to have EOT values as low as 0.5 nm under low temperature processing. After 1000 {degree sign}C, 10 sec anneals consistent with dopant activation, lanthanum silicate is shown by cross-sectional TEM to remain in the amorphous phase, with no La diffusion into the Si substrate as measured by back side SIMS. Lanthanum addition to hafnium-based dielectrics is presently of interest for the purposes of increasing the dielectric constant, and for providing threshold voltage (Vt) control for obtaining band-edge nMOSFET device operation. A 400-500 mV reduction in Vt is obtained using a lanthana cap layer on HfSiON, achieving band edge nMOSFETs with minimal mobility degradation.
Surface Vibrational Excitations on Si(001) 2 × 1 (1984)

We present the first realistic study of the surface-phonon spectrum of a reconstructed semiconductor surface. The extensive reconstruction of the Si(001)2 \ifmmode\times\else\texttimes\fi{} 1 surface is found to localize a rich spectrum of surface modes including a band localized nearly completely on a rocking motion of surface dimers and an unanticipated optical mode localized in the first two subsurface layers. With use of a tight-binding theory for structural energies, the model incorporates the effects of bond rehybridization at the surface.
A Remote Carrier Synchronization Technique for Coherent Distributed Remote Sensing Systems (2020)

Phase, frequency, and time synchronization are crucial requirements for many applications, such as multi-static remote sensing and communication systems. Moreover, the synchronization solution becomes even more challenging when the nodes are orbiting or flying on airborne or spaceborne platforms. This paper compares the available technologies used for the synchronization and coordination of nodes in distributed remote sensing applications. Additionally, this paper proposes a general system model and identifies preliminary guidelines and critical elements for implementing the synchronization mechanisms exploiting the inter-satellite communication link. The distributed phase synchronization loop introduced in this work deals with the self-interference in a full-duplex point to point scenario by transmitting two carriers at each node. All carriers appear with different frequency offsets around a central frequency, called the application central-frequency or the beamforming frequency. This work includes a detailed analysis of the proposed algorithm and the required simulations to verify its performance for different phase noise, AWGN, and Doppler shift scenarios.
Distributed mobility management for future 5G networks: overview and analysis of existing approaches (2015)

The ever-increasing demand of mobile Internet traffic is pushing operators to look for solutions to increase the available bandwidth per user and per unit of area. At the same time, they need to reduce the load in the core network at a reasonable cost in their future 5G deployments. Today's trend points to the deployment of extremely dense networks in order to provide ubiquitous connectivity at high data rates. However, this is hard to couple with the current mobile networks' architecture, which is heavily centralized, posing difficult challenges when coping with the foreseen explosion of mobile data. Additionally, future 5G networks will exhibit disparate types of services, posing different connectivity requirements. Distributed mobility management is emerging as a valid framework to design future mobile network architectures, taking into account the requirements for large traffic in the core and the rise of extremely dense wireless access networks. In this article, we discuss the adoption of a distributed mobility management approach for mobile networks, and analyze the operation of the main existing solutions proposed so far, including a first practical evaluation based on experiments with real Linux-based prototype implementations.
Gone To Pot (2004)

Review of the exhibition Secret History of Clay: From Gauguin to Gormley at Tate Liverpool. Jones asks,Why has modern art in this particular medium been so dismal? He praises the work of Picasso and Gauguin and describes Perry's work as eyesores. A Secret History of Clay: From Gauguin to Gormley, Tate Liverpool, June- August 2004.
Characterization of Full-Range Time Evolution in Active Thermography (2014)

The depth range of active thermography is limited by several factors including the amount of excitation applied to the sample and the noise level of the IR camera. However, the subsurface diffusion process continues, regardless of our ability to monitor it. On a sufficiently large time scale the surface temperature response appears in the logarithmic domain as a transition from an initial 1-dimensional diffusion state to a final one, with the beginning and end of the transition indicated by a peak in the 2 nd derivative. The lifetime of transient diffusion process, during which the usual contrast and peak slope maxima are observed, can be defined as the ratio of the times at which the 2 nd derivative maxima occur.
Toxicity and Efficacy of a Novel GADD34-expressing Oncolytic HSV-1 for the Treatment of Experimental Glioblastoma (2018)

<b>Purpose:</b> Glioblastoma (GBM) is the most common primary central nervous system cancer in adults. Oncolytic HSV-1 (oHSV) is the first FDA-approved gene therapy approach for the treatment of malignant melanoma. For GBM, oHSVs need to be engineered to replicate within and be toxic to the glial tumor but not to normal brain parenchymal cells. We have thus engineered a novel oHSV to achieve these objectives.<b>Experimental Design:</b> NG34 is an attenuated HSV-1 with deletions in the genes encoding viral ICP6 and ICP34.5. These mutations suppress virus replication in nondividing brain neurons. NG34 expresses the human <i>GADD34</i> gene under transcriptional control of a cellular Nestin gene promoter/enhancer element, whose expression occurs selectively in GBM. <i>In vitro</i> cytotoxicity assay and survival studies with mouse models were performed to evaluate therapeutic potency of NG34 against glioblastoma. <i>In vivo</i> neurotoxicity evaluation of NG34 was tested by intracerebral inoculation.<b>Results:</b> NG34 replicates in GBM cells <i>in vitro</i> with similar kinetics as those exhibited by an oHSV that is currently in clinical trials (rQNestin34.5). Dose-response cytotoxicity of NG34 in human GBM panels was equivalent to or improved compared with rQNestin34.5. The <i>in vivo</i> efficacy of NG34 against two human orthotopic GBM models in athymic mice was similar to that of rQNestin34.5, whereas intracerebral injection of NG34 in the brains of immunocompetent and athymic mice showed significantly better tolerability. NG34 was also effective in a syngeneic mouse glioblastoma model.<b>Conclusions:</b> A novel oHSV encoding <i>GADD34</i> is efficacious and relatively nontoxic in mouse models of GBM. <i>Clin Cancer Res; 24(11); 2574-84. ©2018 AACR</i>.
Continuous wave Terahertz generation from photomixer with single walled carbon nanotubes film (2013)

Photomixer with single walled carbon nanotubes utilizing a dual dipole antenna is fabricated. The output spectrum of the continuous-wave Terahertz photomixer with SWCNTs is presented. DC biased voltage dependent characteristic of CW photomixer with SWCNTs is demonstrated.
Location Configuration Protocol (2006)

Location Configuration Protocol provides a host with physical location
based on its IP address. This document describes the communication
protocol and mechanism for an IP host to discovery the physical
location associated with its own IP address by communicating with a
Location Information Server. This document describes the overall
protocol and communication architecture.
On the stress and temperature dependence of low temperature and high stress shear creep in Ni-base single crystal superalloys (2020)

In the present work, we investigate the stress and temperature dependence of low-temperature (750 ± 20 °C) and high-stress (300 ± 20 MPa) shear creep of a Ni-base single crystal superalloy. From continuous isothermal experiments and stress and temperature change tests the stress exponent n and the apparent activation energy Qapp of the phenomenological Sherby-Dorn equation were determined for the two macroscopic crystallographic shear systems (MCSS) [011¯](111) and [112¯](111). The activation parameters of creep, the stress exponents and the apparent activation energies were identified as 16 and 620 kJ/mol (MCSS: [011¯](111)) and 14 and 460 kJ/mol (MCSS: [112¯](111)). We show that during shear creep testing these phenomenological parameters do not change between the early (0.5–1% strain) and later stages of creep (4.5–5% strain), in contrast to what was observed for uniaxial tensile testing. The results are discussed in the light of what is known about stress and temperature dependencies of deformation rates in the creep literature and in view of the recent work by Bürger et al., 2020, who combined shear creep testing with analytical transmission electron microscopy to identify the elementary deformation mechanism, which governs low temperature and high stress creep.
Optimization of Reflectance Pulse Oximetry Sensors Final Report CRADA No. TC-485-93 (2018)

The focus of this Cooperative Research and Development Agreement (CRADA) was to design a next generation of reflectance pulse oximeter sensor. Nellcor wanted to develop an optimized reflectance pulse oximeter sensor with reduced sensitivity to the placement of the sensor (and to composition of the sampled tissue) while accurately measuring the perfusion of the tissue (i.e. oxygen saturation). Nellcor Inc. changed the company name to Nellcor-Puritan Bennett, Inc. in 1995.
Physical Cell ID Detection Probability Using NB-IoT Synchronization Signals in 28-GHz Band (2021)

This paper presents the physical-layer cell identity (PCID) detection probability using the narrowband primary synchronization signal (NPSS) and narrowband secondary synchronization signal (NSSS) based on the narrowband Internet-of-Things (NB-IoT) radio interface considering frequency offset and the maximum Doppler frequency in the 28-GHz band. Simulation results show that the autocorrelation based NPSS detection method is more effective than the cross-correlation based NPSS detection using frequency offset estimation and compensation before the NPSS received timing detection from the viewpoints of PCID detection probability and computational complexity. We also show that when using autocorrelation based NPSS detection, the loss in the PCID detection probability at the carrier frequency of fc =28GHz compared to that for fc =3.5GHz is only approximately 5% at the average received signal-to-noise ratio (SNR) of 0dB when the frequency stability of a local oscillator of a user equipment (UE) set is 20ppm. Therefore, we conclude that the multiplexing schemes and sequences of NPSS and NSSS based on the NB-IoT radio interface associated with autocorrelation based NPSS detection will support the 28-GHz frequency spectra.
Supplemental legend from Exceptional Response to Pembrolizumab in a Metastatic, Chemotherapy/Radiation-Resistant Ovarian Cancer Patient Harboring a PD-L1-Genetic Rearrangement (2023)

&lt;p&gt;Supplemental legend&lt;/p&gt;
Rational Drug Design Leading to the Identification of a Potent 5-HT 2C Agonist Lacking 5-HT 2B Activity (2020)

The 5-HT2C receptor is an attractive drug target in the quest for new therapeutics to treat a variety of human disorders. We have previously undertaken a structural optimization campaign that has led to some potent and moderately selective 5-HT2C receptor agonists. After expanding our structure–function library, we were able to combine our data sets so as to allow the design of compounds of improved selectivity and potency. We disclose herein the structural optimization of our previously reported 5-HT2B/5-HT2C agonists, which has led to the identification of a highly selective 5-HT2C agonist, (+)-trans-[2-(2-cyclopropylmethoxyphenyl)cyclopropyl]methylamine hydrochloride, with an EC50 of 55 nM and no detectable agonism at the 5-HT2B receptor.
Quantifying the interplay between rapid bacterial evolution within the mouse intestine and transmission between hosts (2020)

Summary Due to limitations on high-resolution strain tracking, selection dynamics during gut-microbiota colonization and transmission between hosts remain mostly mysterious. Here, we introduced hundreds of barcoded Escherichia coli strains into germ-free mice and quantified strain-level dynamics and metagenomic changes. Mutants involved in motility and utilization of abundant metabolites were reproducibly selected within days. Even with rapid selection, coprophagy enforced similar barcode distributions across co-housed mice. Whole-genome sequencing of hundreds of isolates quantified evolutionary dynamics and revealed linked alleles. A population-genetics model predicted substantial fitness advantages for certain mutants and that migration accounted for ~10% of the resident microbiota each day. Treatment with ciprofloxacin demonstrated the interplay between selection and transmission. While initial colonization was mostly uniform, in two mice a bottleneck reduced diversity and selected for ciprofloxacin resistance in the absence of drug. These findings highlight the interplay between environmental transmission and rapid, deterministic selection during evolution of the intestinal microbiota.
The effect of low earth orbit atomic oxygen exposure on phenylphosphine oxide-containing poly(arylene ether)s (1998)

Unoriented thin films of phenylphosphine oxide-containing poly(arylene ether)s were exposed to low Earth orbit aboard the space shuttle Atlantis (STS-51) as part of a flight experiment designated Limited Duration Candidate Exposure (LDCE 4–5). The samples were exposed to primarily atomic oxygen (!10,\7×1019 atoms/cm2). Based on post-flight analyses using atomic force microscopy, X-ray photoelectron spectroscopy, gel permeation chromatogrpahy and weight loss data, it was proposed that atomic oxygen exposure of these materials produces a phosphate layer at the surface of the samples, apparently by the reaction of atomic oxygen with the phosphorus in the polymer backbone. Ground-based oxygen plasma exposure experiments have previously shown that this phosphate layer provides a barrier against further attack by atomic oxygen [1]. The results obtained from these analyses compare favorably with those obtained from samples exposed to an oxygen plasma in ground-based exposure experiments [1]. © 1998 John Wiley & Sons, Ltd.
Weakly Supervised Deep Recurrent Neural Networks for Basic Dance Step Generation (2018)

Synthesizing human's movements such as dancing is a flourishing research field which has several applications in computer graphics. Recent studies have demonstrated the advantages of deep neural networks (DNNs) for achieving remarkable performance in motion and music tasks with little effort for feature pre-processing. However, applying DNNs for generating dance to a piece of music is nevertheless challenging, because of 1) DNNs need to generate large sequences while mapping the music input, 2) the DNN needs to constraint the motion beat to the music, and 3) DNNs require a considerable amount of hand-crafted data. In this study, we propose a weakly supervised deep recurrent method for real-time basic dance generation with audio power spectrum as input. The proposed model employs convolutional layers and a multilayered Long Short-Term memory (LSTM) to process the audio input. Then, another deep LSTM layer decodes the target dance sequence. Notably, this end-to-end approach has 1) an auto-conditioned decode configuration that reduces accumulation of feedback error of large dance sequence, 2) uses a contrastive cost function to regulate the mapping between the music and motion beat, and 3) trains with weak labels generated from the motion beat, reducing the amount of hand-crafted data. We evaluate the proposed network based on i) the similarities between generated and the baseline dancer motion with a cross entropy measure for large dance sequences, and ii) accurate timing between the music and motion beat with an F-measure. Experimental results revealed that, after training using a small dataset, the model generates basic dance steps with low cross entropy and maintains an F-measure score similar to that of a baseline dancer.