gebhart/patrepeval huggingface dataset:
Identification and Characterization of Tyrosine Kinase Nonreceptor 2 Mutations in Leukemia through Integration of Kinase Inhibitor Screening and Genomic Analysis (2016)

The amount of genomic information about leukemia cells currently far exceeds our overall understanding of the precise genetic events that ultimately drive disease development and progression. Effective implementation of personalized medicine will require tools to distinguish actionable genetic alterations within the complex genetic landscape of leukemia. In this study, we performed kinase inhibitor screens to predict functional gene targets in primary specimens from patients with acute myeloid leukemia and chronic myelomonocytic leukemia. Deep sequencing of the same patient specimens identified genetic alterations that were then integrated with the functionally important targets using the HitWalker algorithm to prioritize the mutant genes that most likely explain the observed drug sensitivity patterns. Through this process, we identified tyrosine kinase nonreceptor 2 (TNK2) point mutations that exhibited oncogenic capacity. Importantly, the integration of functional and genomic data using HitWalker allowed for prioritization of rare oncogenic mutations that may have been missed through genomic analysis alone. These mutations were sensitive to the multikinase inhibitor dasatinib, which antagonizes TNK2 kinase activity, as well as novel TNK2 inhibitors, XMD8-87 and XMD16-5, with greater target specificity. We also identified activating truncation mutations in other tumor types that were sensitive to XMD8-87 and XMD16-5, exemplifying the potential utility of these compounds across tumor types dependent on TNK2. Collectively, our findings highlight a more sensitive approach for identifying actionable genomic lesions that may be infrequently mutated or overlooked and provide a new method for the prioritization of candidate genetic mutations.
Low-cost ultra-fast DC circuit-breaker: Power electronics integrated with mechanical switchgear (2018)

The paper describes a concept for fast-acting circuit-breaker using vacuum interrupter (VI) assisted by a power-electronic converter with low output voltage. The converter excites a passive resonant circuit to create current zero-crossing in the arc in the VI to make it extinguish the current. A fast-acting actuator using a Thomson coil separates the contacts in a few milliseconds. The principle is outlined, and a laboratory prototype is described. In experiments 10 kA was successfully interrupted against 9 kV in 3 ms.
Performance Problems of Non-Toroidal Shaped Current Transformers (2020)

Nowadays, non-toroidal shape primary pass-through current transformers are commonly used for large current machines with several cables per phase. As these transformers exhibit no radial symmetry, it is not clear if they can be tested using the indirect test described in the IEC 61869 standard. In order to answer this question, two non-toroidal shaped current transformers of different secondary winding designs have been tested and simulated. One transformer has a uniformly distributed secondary winding and the other has a partially distributed secondary winding. Both transformers have the same nameplate characteristics. Both perform correctly in the indirect test. However, only the transformer with the uniformly distributed secondary winding performs correctly in a direct test. A finite element simulation shows that the iron core of the partially distributed secondary winding transformer was saturated, while the iron core of the uniformly distributed one was not. This result explains their different performance. The main conclusion is that the indirect test is not sensitive enough to cover all cases and therefore under doubtful situations, the transformers should be tested using the direct test.
Capillary Trapping of CO2 in Sandstone Using Low Field NMR Relaxometry (2019)

Abstract Injecting carbon dioxide into geological formations for long‐term storage is considered integral to reducing greenhouse gas emissions. Residual trapping of CO 2 is a primary storage mechanism, whereby CO 2 ganglia are trapped in the pore space by capillary forces. Experimental knowledge of residual trapping processes in rocks is critical to the development of safe storage strategies. Here we present a quantitative low field 1 H nuclear magnetic resonance (NMR) core flooding study of CO 2 residual trapping in three different sandstones. It was found that transverse relaxation ( T 2 ) measurements were sensitive to the dissolution of paramagnetic ions from rock matrix minerals after exposure to carbonic acid; this response was observed on time scales relevant to core flooding experiments (i.e., minutes to hours). Subsequently, a brine aging protocol was designed and implemented to minimize this chemical effect, and hence, by applying the well‐known T 2 ‐pore size relationship, changes in T 2 time distributions during core flooding could be related to displacement of brine from pores of different sizes. Comparison of T 2 distributions for partially CO 2 /brine‐saturated cores to previously published data for N 2 /H 2 O systems shows an increased displacement of brine from small pores by CO 2 . Furthermore, results from cyclical brine/CO 2 injections showed an increase in the total volume of residually trapped CO 2 and an increase in trapping efficiency; compared to the results observed for N 2 /H 2 O, however, the improvement in trapping efficiency with cyclic injection was less pronounced. Potential causes for the observed differences are discussed in the context of effective N 2 and CO 2 wetting.
Critical role of CdSe nanoplatelets in color-converting CdSe/ZnS nanocrystals for InGaN/GaN light-emitting diodes (2016)

Here we report CdSe nanoplatelets that are incorporated into color-converting CdSe/ZnS nanocrystals for InGaN/GaN light-emitting diodes. The critical role of CdSe nanoplatelets as an exciton donor for the color conversion was experimentally investigated. The power conversion efficiency of the hybrid light-emitting diode was found to increase by 23% with the incorporation of the CdSe nanoplatelets. The performance enhancement is ascribed to efficient exciton transfer from the donor CdSe nanoplatelet quantum wells to the acceptor CdSe/ZnS nanocrystal quantum dots through Förster-type nonradiative resonance energy transfer.
Regulation of immunoglobulin transcription. (1991)

B lymphocytes express membrane-bound immunoglobulin molecules as antigen-specific, clonally distributed, cell surface receptors. Molecules with the same fine specificity can be secreted in large amounts into the body fluids by progeny of the clone, subsequent to antigenic challenge and appropriate stimulation into terminal differentiation. While this review is limited to the regulation of immunoglobulin transcription, it should be noted that immunoglobulin expression is regulated at the level of DNA, RNA and protein. A DNA recombination process determines the specificity of the antibody to be expressed, the immunoglobulin RNA level is regulated qualitatively and quantitatively, as is the mature immunoglobulin protein.
Abstract 17946: How Common is New Onset Atrial Fibrillation in Single Chamber ICD Patients? Sub-analysis From the PainFree SST Study (2015)

Introduction: Nearly 75% of ICD recipients have no history of atrial fibrillation (AF) at the time of initial implantation. Yet, many of these patients will develop new onset AF after implant which can lead to serious consequences if not detected and treated. To date, detection algorithms in single-chamber ICDs are not able to detect AF. We investigate how common is new onset AF in single chamber (SC) ICD recipients compared to CRT-D and dual chamber (DC) ICD recipients. Methods: A total of 924 patients with no history of AF from the PainFree SST study (CRT-D: N=321, DC ICD: N=303, SC ICD: N=300) were investigated. We used data from dual-chamber ICDs to estimate the incidence of new-onset AT/AF in SC ICD patients. A propensity score weighted analysis was performed using DC ICD data to match the baseline data of SC ICD patients. Patients with pacing indication were excluded from this evaluation because they would not be indicated for SC ICD. AT/AF-related complications include AT/AF-related serious adverse events, thromboembolic events, progress to AF lasting 7 days or more as well as inappropriate shocks due to AT/AF. Simulated SC ICD results were compared to actual DC and CRT-D results. Results: For AF episodes lasting 6 minutes or more, the incidence rates in CRT-D, DC ICD, and the estimated SC ICD were 28.0%, 23.4%, and 20.5% at 24 months, respectively (Table). The estimated incidence rates of AT/AF episodes in patients with SC ICD were comparable to those of DC ICD in each episode duration. Once the patients experienced first AT/AF episode, the complications were common (Table). Conclusions: AT/AF was frequently detected following implant in patients with no AF history receiving ICD or CRT-D and is associated subsequent AT/AF-related complications even in SC ICD recipients. Therefore, AT/AF monitoring in all ICDs patients may identify those at risk for these complications.
Immobilization of Gold Nanorods onto Electrospun Polycaprolactone Fibers Via Polyelectrolyte Decoration—A 3D SERS Substrate (2013)

We report the fabrication of a homogeneous and highly dense gold nanorod (AuNR) assembly on electrospun polycaprolactone (PCL) fibers using electrostatic interaction as the driving force. Specifically, decoration of a poly(sodium 4-styrenesulfonate) (PSS) layer onto the AuNRs imposed negative charges on the nanorod surface, and the interactions between PSS and the AuNRs were investigated using attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR). Positive charges on the PCL fibrous substrate were established via polyelectrolyte layer-by-layer deposition, which was investigated using multiple characterization techniques. Driven by the attractive electrostatic interaction, immobilization of AuNRs on the PCL fibers was initiated upon substrate immersion, and the kinetics of the immobilization process were studied using UV-vis spectroscopy. Electron microscopy characterization of the AuNR/PCL nanocomposite fibers reveals a uniform AuNR coating on the fiber surface with the immobilized AuNR density being high enough to provide full surface coverage. By using both 4-mercaptopyridine and Rhodamine 6G as probe molecules, the performance of the AuNR/PCL fibrous mesh as a three-dimensional (3D) surface-enhanced Raman scattering (SERS) substrate was investigated. The nanocomposite fibers allowed detection at concentrations as low as 10(-7) M of the probe molecule in solution and exhibited excellent reproducibility in the SERS measurements. In addition, a comparison between the 3D AuNR/PCL fibrous mesh and a 2D AuNR/PCL film reveals that the enhanced surface area in the 3D substrate effectively improved the SERS performance with a 6-fold increase in the Raman intensity.
Computations and 1H NMR Spectroscopy of the Imide Region Can Distinguish Isomers of Hydrogen-Bonded Aggregates (1997)

ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTComputations and 1H NMR Spectroscopy of the Imide Region Can Distinguish Isomers of Hydrogen-Bonded AggregatesDonovan N. Chin, Eric E. Simanek, Xinhua Li, Mohammed I. M. Wazeer, and George M. WhitesidesView Author Information Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138 Cite this: J. Org. Chem. 1997, 62, 6, 1891–1895Publication Date (Web):March 21, 1997Publication History Received21 June 1996Published online21 March 1997Published inissue 1 March 1997https://pubs.acs.org/doi/10.1021/jo961176fhttps://doi.org/10.1021/jo961176fbrief-reportACS PublicationsCopyright © 1997 American Chemical SocietyRequest reuse permissionsArticle Views218Altmetric-Citations8LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Group theory,Melamine,Molecular structure,Molecules,Stability Get e-Alerts
On the prediction of fatigue crack growth based on weight functions in residual stress fields induced by laser shock peening and laser heating (2021)

Abstract This study deals with fatigue crack growth prediction for thin sheet AA2024 specimens with residual stresses introduced through laser shock peening and laser heating treatments. Different weight functions are used to calculate the stress intensity factor due to the presence of residual stresses. A superposition principle is used by calculating the total stress intensity factor considering the applied loads and residual stresses. The fatigue crack growth is predicted using the Paris' law based on the effective stress intensity factor range, in that the effect of residual stresses is considered by changing the total stress intensity factor ratio. It is concluded, that weight functions are a powerful tool to predict fatigue crack growth in AA2024 containing residual stress fields, as long as the gradient in the loading direction, as induced by laser shock peening and laser heating, is moderate.
Synthesis of [3,5-14C]Trachelanthamidine and [5-3H]Isoretronecanol and Their Incorporation into the Retronecine Moiety of Riddelliine in Senecio riddellii (1986)

(+/-)-[3,5-14C]Trachelanthamidine and (+/-)-[5-3H]isoretronecanol, which are diastereomers, were prepared from potassium [14C]cyanide and [5-3H]proline, respectively. These compounds and [1,4-14C]putrescine were administered to Senecio riddellii plants resulting in the formation of labeled riddelliine, in which almost all the radioactivity was located in its retronecine moiety. The activity of the beta-alanine obtained by degradation of the retronecine was consistent with specific labeling of this pyrrolizidine base at the expected positions. The extremely high absolute incorporation (15.1, 22.1%) of trachelanthamidine into riddelliine strongly favors this 1-hydroxymethylpyrrolizidine as the one on the main biosynthetic pathway to retronecine. The lower incorporation (0.75%) of isoretronecanol may represent a minor or aberrant pathway to retronecine.
Synthesis of Perfluorinated Ketones by Utilizing Liquid‐Phase Direct Fluorination. (2007)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Development and Characterisation of a Four-Plex Assay to Measure Streptococcus pyogenes Antigen-Specific IgG in Human Sera (2022)

The measurement of antibodies to vaccine antigens is crucial for research towards a safe and effective vaccine for <i>Streptococcus pyogenes</i> (Strep A). We describe the establishment and detailed characterisation of a four-plex assay to measure IgG to the Strep A vaccine antigens SpyCEP, Slo, SpyAD and GAC using the Luminex multiplex platform. A standard curve was established and characterized to allow the quantification of antigen-specific IgG. Assay specificity, precision, linearity, reproducibility and repeatability were determined via the measurement of antigen-specific IgG from pooled human serum. The assay is highly specific, reproducible and performs well across a large range of antibody concentrations against all four antigens. It is, therefore, suitable for future clinical trials in humans with a four-component vaccine, as well as for seroepidemiological studies to gain insights into naturally occurring immunity.
Eμ-BCL10 mice exhibit constitutive activation of both canonical and noncanonical NF-κB pathways generating marginal zone (MZ) B-cell expansion as a precursor to splenic MZ lymphoma (2009)

BCL10, required for nuclear factor kappaB (NF-kappaB) activation during antigen-driven lymphocyte responses, is aberrantly expressed in mucosa-associated lymphoid tissue-type marginal zone (MZ) lymphomas because of chromosomal translocations. Emu-driven human BCL10 transgenic (Tg) mice, which we created and characterize here, had expanded populations of MZ B cells and reduced follicular and B1a cells. Splenic B cells from Tg mice exhibited constitutive activation of both canonical and noncanonical NF-kappaB signaling pathways is associated with increased expression of NF-kappaB target genes. These genes included Tnfsf13b, which encodes the B-cell activating factor (BAFF). In addition, levels of BAFF were significantly increased in sera from Tg mice. MZ B cells of Tg mice exhibited reduced turnover in vivo and enhanced survival in vitro, indicative of lymphoaccumulation rather than lymphoproliferation as the cause of MZ expansion. In vivo antibody responses to both T-independent, and especially T-dependent, antigens were significantly reduced in Tg mice. Mortality was accelerated in Tg animals, and some mice older than 8 months had histologic and molecular findings indicative of clonal splenic MZ lymphoma. These results suggest that, in addition to constitutive activation of BCL10 in MZ B cells, other genetic factors or environmental influences are required for short latency oncogenic transformation.
Hybrid Amyloid‐Based Redox Hydrogel for Bioelectrocatalytic H2 Oxidation (2021)

Abstract An artificial amyloid‐based redox hydrogel was designed for mediating electron transfer between a [NiFeSe] hydrogenase and an electrode. Starting from a mutated prion‐forming domain of fungal protein HET‐s, a hybrid redox protein containing a single benzyl methyl viologen moiety was synthesized. This protein was able to self‐assemble into structurally homogenous nanofibrils. Molecular modeling confirmed that the redox groups are aligned along the fibril axis and are tethered to its core by a long, flexible polypeptide chain that allows close encounters between the fibril‐bound oxidized or reduced redox groups. Redox hydrogel films capable of immobilizing the hydrogenase under mild conditions at the surface of carbon electrodes were obtained by a simple pH jump. In this way, bioelectrodes for the electrocatalytic oxidation of H 2 were fabricated that afforded catalytic current densities of up to 270 μA cm −2 , with an overpotential of 0.33 V, under quiescent conditions at 45 °C.
Melt Rheology (2017)

No AccessUnderstanding Polymer ProcessingOct 2017Melt RheologyTim A. OsswaldTim A. OsswaldSearch for more papers by this authorhttps://doi.org/10.3139/9781569906484.003SectionsAboutPDF ToolsAdd to FavoritesDownload CitationTrack CitationsCopy LTI LinkPDF key 'share (en)' returned an object instead of string.FacebookTwitterEmailLinkedIn previous chapternext chapter FiguresReferencesRelatedDetails 2017Pages: 61-76Print ISBN: 978-1-56990-647-7eISBN: 978-1-56990-648-4 Copyright & Permissions© 2017 Carl Hanser Verlag GmbH & Co. KGPDF downloadLoading ...
Combination of compressed sensing and parallel imaging with respiratory motion correction for highly-accelerated cardiac perfusion MRI (2011)

Cardiac perfusion MRI requires fast data acquisition to achieve an appropriate combination of temporal resolution, spatial resolution and spatial coverage for clinical studies [1]. We have recently presented a combination of compressed sensing and parallel imaging (k-t SPARSE-SENSE) to highly accelerate perfusion studies [2]. However, this method is sensitive to respiratory motion, which decreases temporal sparsity and produces temporal blurring in the reconstructed images. In this work, we present a rigid respiratory motion correction method which allows highly-accelerated first-pass cardiac perfusion MRI to be performed without strict breath-holding.
REBUILDING ANTI-MALWARE TESTING FOR THE FUTURE (2008)

This paper discusses several aspects related to testing the ability of security products to detect malware. The complexity of malware and of security solutions continues to increase extremely quickly, so we present arguments as to why we believe that traditional comprehensive testing of anti-virus products (QA) is no longer viable and why a different approach is in order. We look into the problem of compiling a representative ‘next-generation’ sample test set:
Review of Patent Publications from 1990 to 2010 on Catalytic Coatings on Different Substrates, Including Microstructured Channels: Preparation, Deposition Techniques, Applications (2012)

A review of patent publications on preparation, deposition, characterization, and application of the catalytic coatings is given in this paper with view on their application in microstructured reactors for heterogeneous gas-phase processing. The paper consists of two main parts: in the first part the total overview of patents, the distribution by countries and patent applicants is shown. The main topics (coatings preparation, application, removal etc.) are discussed in the second part of the review, including description of some selected patent publications. The aim of the present review is to show the ‘hot’ topics of patent publications on catalytic coatings and the main interests of the big patentees; special attention is given to catalytic coatings on the channel walls in microstructured reactors. Keywords: Catalytic coatings, deposition techniques, microstructured reactors, patents.
Activity of TNF‐related apoptosis‐inducing ligand (TRAIL) in haematological malignancies (1997)

T-cell cytotoxicity is primarily mediated by two cell surface proteins, Fas ligand (FasL) and tumour necrosis factor-related apoptosis-inducing ligand (TRAIL), and intracellular perforin and granzyme granules. FasL-deficient and perforin-deficient T lymphocytes maintain cytotoxicity but fail to induce graft-versus-host disease (GVHD) when transplanted into mice. suggesting that GVHD and graft-versus-tumour (GVT) effects can be dissociated, and that TRAIL is not involved in the pathogenesis of GVHD. Because TRAIL could mediate a favourable GVT effect it became important to study the spectrum of its activity and to investigate factors that can dissociate its expression from FasL. TRAIL induced apoptosis in 11/41 (27%) tumour specimens of haematological origin compared to 16/41 (39%) induced by FasL. Although eight specimens were sensitive to both FasL and TRAIL, no synergism was observed between these two ligands. TRAIL induced apoptosis in a dose and time dependent manner with an ED50 of 0.5 microg/ml and EDmax of 1 microg/ml. TRAIL activity was not reduced by the over-expression of the multidrug resistant (MDR) protein, and was not enhanced by 9-cis retinoic acid (RA), which can down-regulate bcl-2 protein. Both ligands were simultaneously up-regulated in normal peripheral blood lymphocytes in response to IL-2, IL-15 and anti-CD3 antibody, whereas IL-10 had no effect. Together, our data show that (1) TRAIL can mediate cell death in a variety of human haematological malignancies, (2) resistance to TRAIL is not mediated by MDR protein, (3) the lack of synergy between TRAIL and FasL suggests that either one is sufficient to mediate T-cell cytotoxicity, and (4) within the panel of cytokines tested, the expression of TRAIL and FasL could not be dissociated.