gebhart/patrepeval huggingface dataset:
Structures of aminoglycoside acetyltransferase AAC(6′)-Ii in a novel crystal form: structural and normal-mode analyses (2005)

The aminoglycoside-modifying enzyme aminoglycoside 6'-N-acetyltransferase type Ii [AAC(6')-Ii] has been crystallized with its cofactor coenzyme A in space group C222(1), with unit-cell parameters a = 71.5, b = 127.4, c = 76.9 A and one physiologically relevant dimer species per asymmetric unit. The space group previously observed for this complex was P2(1)2(1)2(1), with two dimers per asymmetric unit. By comparing the six available protomer structures of the AAC(6')-Ii-CoA complex, it has been possible to identify regions of plasticity within the protein. Normal-mode analysis of this complex suggests that this plasticity is not an artefact of crystal-packing forces, but that the region of the protomer that displays multiple conformations is intrinsically flexible. It is conjectured that the flexibility is relevant for the cooperative activity observed for the enzyme.
[Echocardiography and biventricular resynchronisation]. (2005)

Biventricular resynchronisation has been recently shown to be an effective therapeutic option in patients with refractory dilated cardiomyopathy. Based on the finding of ventricular asynchrony, the aim of the method is to restore uniform contraction of the ventricular walls. However, the initial electrocardiographic criteria for selection of patients were only associated with a 70% rate of response. Consequently, it became necessary to refocus this method in patients with true ventricular asynchrony. Echocardiography is one of the non-invasive techniques which provides morphological and functional analysis of the myocardium with a high degree of accessibility. The multiplication of tools for echocardiographic quantification has been very valuable from a theoretical point of view for assessing ventricular asynchrony. In practice, techniques such as Doppler tissue imaging are being validated, but already offer the possibility of a multi-directional approach to this pathology. The diagnosis of asynchrony is based on a range of echocardiographic findings which not only improve the selection of patients but also optimise the programming of multisite stimulation.
Use of Molecular Symmetry To Describe Pauli Principle Effects on the Vibration–Rotation Spectroscopy of CO2(g) (2004)

A previous article published in this Journal (2002, 79, 117) described the vibration–rotation spectroscopy of the asymmetric stretching fundamental infrared CO2 absorbance and its use in an undergraduate laboratory. The consequence of the nuclear spin of oxygen (I = 0) on the rotational fine structure of this absorbance is to forbid alternate rotational levels, causing the observed spacing between rotation–vibration lines to be twice that expected for linear nonhomonuclear molecules such as HCl. The present article presents a systematic method for obtaining the Pauli principle rules based on the analysis of a series of symmetry operations equivalent to the exchange of identical oxygen nuclei in CO2.
Preparation of NHC Borane Complexes by Lewis Base Exchange with Amine− and Phosphine−Boranes (2010)

A versatile new method for the preparation of NHC boranes starting from two stable, readily available reactants-an heterocyclic salt and an amine or phosphine-borane-is reported. It uses a Lewis base exchange at boron and provides easy access to new NHC boranes, in particular B-substituted borane ones.
Physical mapping of the chromosome 7 breakpoint region in an SLOS patient with t(7;20) (q32.1;q13.2) (1997)

Smith-Lemli-Opitz syndrome (SLOS) is an autosomal recessive disorder characterized by multiple congenital anomalies and mental retardation. SLOS has an associated defect in cholesterol biosynthesis, but the molecular genetic basis of this condition has not yet been elucidated. Previously our group reported a patient with a de novo balanced translocation [t(7;20)(q32.1;q13.2)] fitting the clinical and biochemical profile of SLOS. Employing fluorescence in situ hybridization (FISH), a 1.8 Mb chromosome 7-specific yeast artificial chromosome (YAC) was identified which spanned the translocation breakpoint in the reported patient. The following is an update of the on-going pursuit to physically and genetically map the region further, as well as the establishment of candidate genes in the 7q32.1 breakpoint region. Am. J. Med Genet. 68:279–281, 1997. © 1997 Wiley-Liss, Inc.
Supplementary Methods from Single-Chain Antibody-Based Immunotoxins Targeting Her2/neu: Design Optimization and Impact of Affinity on Antitumor Efficacy and Off-Target Toxicity (2023)

&lt;p&gt;PDF file - 155KB&lt;/p&gt;
Design Characteristics of a Raman Silicon Nanocavity Laser for Efficient Emission of Light Into an Adjacent Waveguide (2021)

Our numerical investigation indicates that, in a Raman Si nanocavity laser, optimized quality factors and waveguides with heterointerfaces are required for efficient emission of the laser light into the planar waveguide for the Stokes light.
High-Throughput Quantitation of Cannabinoids by Liquid Chromatography Triple-Quadrupole Mass Spectrometry (2022)

The high-throughput quantitation of cannabinoids is important for the cannabis industry. As medicinal products increase, and research into compounds that have pharmacological benefits increase, and the need to quantitate more than just the main cannabinoids becomes more important. This study aims to provide a rapid, high-throughput method for cannabinoid quantitation using a liquid chromatography triple-quadrupole mass spectrometer (LC-QQQ-MS) with an ultraviolet diode array detector (UV-DAD) for 16 cannabinoids: CBDVA, CBDV, CBDA, CBGA, CBG, CBD, THCV, THCVA, CBN, CBNA, THC, Δ8-THC, CBL, CBC, THCA-A and CBCA. Linearity, limit of detection (LOD), limit of quantitation (LOQ), accuracy, precision, recovery and matrix effect were all evaluated. The validated method was used to determine the cannabinoid concentration of four different <i>Cannabis sativa</i> strains and a low THC strain, all of which have different cannabinoid profiles. All cannabinoids eluted within five minutes with a total analysis time of eight minutes, including column re-equilibration. This was twice as fast as published LC-QQQ-MS methods mentioned in the literature, whilst also covering a wide range of cannabinoid compounds.
Identification of a major maturation-activated acetyl-CoA carboxylase kinase in sea star oocytes as p44mpk (1991)

Maturation-activated protein-serine/threonine kinases were investigated in the high-speed supernatant fractions from sea-star oocytes harvested at the time of germinal vesicle breakdown. One of the major stimulated protein kinases able to phosphorylate acetyl-CoA carboxylase in these extracts was found to co-purify with a 44 kDa myelin basic protein kinase (p44mpk) that is activated with a similar time course during oocyte maturation. Purified sea-star oocyte p44mpk phosphorylated acetyl-CoA carboxylase (purified from rat liver) predominantly on serine and to a small extent on threonine. Furthermore, the phosphorylation of acetyl-CoA carboxylase occurred principally on a tryptic phosphopeptide which displayed electrophoretic and chromatographic properties very similar to those of the peptide that has previously been shown to undergo increased phosphorylation in response to insulin in rat adipocytes [Brownsey & Denton (1982) Biochem. J. 202, 77-86]. The acetyl-CoA carboxylase was phosphorylated at a similar rate and to a similar extent by casein kinase II, which was also purified from maturing sea-star oocytes. Although casein kinase II was also activated approximately 3-fold near the time of nuclear envelope breakdown, it was responsible for only a minor component of the total enhanced acetyl-CoA carboxylase kinase activity measured in the soluble extracts from maturing oocytes. Acetyl-CoA carboxylase was a relatively poor substrate for the major S6 peptide kinase activity that was also stimulated during resumption of meiosis in the oocytes. The properties of the p44mpk are reminiscent of those of a microtubule-associated protein 2 (MAP-2) kinase that is activated in response to insulin and other mitogens in mammalian cells [Ray & Sturgill (1988) Proc. Natl. Acad. Sci. U.S.A. 85, 3753-3757; Hoshi, Nishida & Sakai (1988) J. Biol. Chem. 263, 5396-5401]. It is intriguing that several of the mammalian protein kinases that are acutely activated after mitogenic prompting of quiescent mouse fibroblasts (i.e. G0 to G1 transition), such as MAP-2 kinase, casein kinase II and S6 kinase II, have counterparts that are activated during M-phase in maturing sea star oocytes.
A Drosophila model for primary coenzyme Q deficiency and dietary rescue in the developing nervous system (2010)

Coenzyme Q (CoQ) or ubiquinone is a lipid component of the electron transport chain required for ATP generation in mitochondria. Mutations in CoQ biosynthetic genes are associated with rare but severe infantile multisystemic diseases. CoQ itself is a popular over-the-counter dietary supplement that some clinical and rodent studies suggest might be beneficial for neurodegenerative diseases. Here, we identify mutations in the Drosophila qless gene, which encodes an orthologue of the human PDSS1 prenyl transferase that synthesizes the isoprenoid side chain of CoQ. We show that neurons lacking qless activity upregulate markers of mitochondrial stress and undergo caspase-dependent apoptosis. Surprisingly, even though experimental inhibition of caspase activity did not prevent mitochondrial disruption, it was sufficient to rescue the size of neural progenitor clones. This demonstrates that, within the developing larval CNS, qless activity is required primarily for cell survival rather than for cell growth and proliferation. Full rescue of the qless neural phenotype was achieved by dietary supplementation with CoQ4, CoQ9 or CoQ10, indicating that a side chain as short as four isoprenoid units can provide in vivo activity. Together, these findings show that Drosophila qless provides a useful model for studying the neural effects of CoQ deficiency and dietary supplementation.
Seamless Wireless Communication Platform for Internet of Things Applications (2022)

The rapid growth in the number of Internet of Things (loT) devices has resulted in the proliferation of wireless technologies to cater to their increasing data rate requirements and to support multiple applications. However, such ever-increasing wireless technologies present numerous challenges such as incompatible wireless standards, increased energy consumption, and insecure communication. The traditional gateways proposed in the literature suffer from limitations such as computational complexity, resource requirements, increased cost, and device size. We envision an era of seamless wireless communication to alleviate the aforementioned challenges in loT applications through three inter-dependent functionalities: detection and identification of wireless technologies, energy-efficient transmit power control, and secure end-to-end communication. To test the concept, a new gateway is proposed that will achieve these three functionalities using only physical layer measurements so that the different communication protocols in the higher layers can be avoided. Novel schemes are conceptualized for resource-limited seamless loT applications. Moreover, the conceptual seamless loT platform is validated through software-based computer simulation and software-defined radio-based testbed implementation. The preliminary analysis demonstrates that the proposed platform has great potential in advancing seamless loT applications.
Experimental Soft Error Rate of Several Flip-Flop Designs Representative of Production Chip in 32 nm CMOS Technology (2013)

This paper shows alpha experimental Soft Error Rate characterization of several standard and hardened Flip-Flop architectures processed in a 32 nm technology. It showsthe effecton the alpha Soft Error Rateof experimental parameters such as algorithm (static vs.kdynamic), data filling the register, etc. 12 data patterns onmore than 5Flip-Floptypes(including DICE-like design)are reported in this articlein order to help the radiation engineer to choose the best algorithm/pattern for its SER characterizations.
Molecular Imprinting Inside Dendrimers (2003)

Synthetic hosts capable of binding porphyrins have been produced by a mixed-covalent-noncovalent imprinting process wherein a single binding site is created within cross-linked dendrimers. Two synthetic hosts were prepared, using as templates 5,10,15,20-tetrakis(4-hydroxyphenyl)porphyrin and 5,10,15,20-tetrakis(3,5-dihydroxyphenyl)porphyrin. These two templates were esterified with, respectively, fourth- and third-generation Fréchet-type dendrons containing homoallyl end-groups. The resulting tetra- and octadendron macromolecules underwent the ring-closing metathesis reaction using Grubbs' Type I catalyst, RuCl(2)(P(C(6)H(5))(3))(2)(CHCH(2)C(6)H(5)), to give extensive interdendron cross-linking. Hydrolytic removal of the porphyrin cores afforded imprinted hosts whose ability to bind porphyrins with various peripheral substituents was investigated by UV-visible spectrophotometric titrations and size exclusion chromatography. The results indicate a high yield of imprinted sites that show high selectivity for binding of porphyrins capable of making at least four hydrogen bonds, but only a moderate degree of shape selectivity.
Ornithine Decarboxylase: Essential in Proliferation But Not Differentiation of Human Promyelocytic Leukemia Cells (1982)

The ornithine decarboxylase inhibitor DL-alpha-difluoromethyl ornithine inhibited a proliferation-associated increase in ornithine decarboxylase activity in cultured human promyelocytic leukemia cells, resulting in a marked suppression of cell proliferation and subsequent cell loss. It also inhibited increases in ornithine decarboxylase activity associated with the phorbol ester-induced conversion of promyelocytic HL-60 cells to monocyte-like cells and the retinoic acid-induced conversion to granulocyte-like cells. However, the inhibition of ornithine decarboxylase activity did not prevent cellular differentiation. These results suggest that polyamine biosynthesis has a specific role in cell proliferation rather than in inducing differentiation that is not accompanied by proliferation. The data also demonstrate that cessation of proliferation in HL-60 cells is not necessarily associated with differentiation.
Clinical review 72: diagnosis and management of functioning islet cell tumors. (1995)

Functional islet cell tumors cause recognizable clinical syndromes based on the peptide products they secrete (Table 1). Frequently, the diagnosis of these tumors is delayed. A high index of suspicion coupled with the use of appropriate biochemical and provocative tests should lead to earlier diagnosis. A suggested management plan for patients suspected of having an islet cell tumor is shown in Fig. 1. Earlier diagnosis coupled with advances in preoperative and intraoperative localization techniques have resulted in an increased number of patients being cured.
Erratum: Erratum to : Design and Construction of New Potentiometric Sensors for Determination of Copper(II) Ion Based on Copper Oxide Nanoparticles [Int. J. Electrochem. Sci., 10 (2015) 7275] (2015)

This is an erratum regarding the published article (Int. J. Electrochem. Sci., 10 (2015) 7275), which has a mistake in the conclusion part.
Nanointegrated, High-Performing Cobalt-Free Bismuth-Based Composite Cathode for Low-Temperature Solid Oxide Fuel Cells (2018)

Cost-effective cathodes that actively catalyze the oxygen reduction reaction (ORR) are one of the major challenges for the technological advancement of low-temperature solid oxide fuel cells (LT-SOFCs). In particular, cobalt has been an essential element in electrocatalysts for efficiently catalyzing the ORR; nevertheless, the cost, safety, and stability issues of cobalt in cathode materials remain a severe drawback for SOFC development. Here, we demonstrated that by appropriate nanoengineering, we can overcome the inherent electrocatalytic advantages of cobalt-based cathodes to achieve comparable performance with a cobalt-free electrocatalyst on a bismuth-based fast oxygen ion-conducting scaffold that simultaneously enhances the performance and stability of LT-SOFCs. Consequently, the peak power density of the SOFCs reaches 1.2 W/cm<sup>2</sup> at 600 °C, highest performance of a cobalt-free-based cathode that has been ever reported. In addition, by the surface-protecting effect of covered nanoelectrocatalysts, the evaporation of highly volatile bismuth is greatly suppressed, resulting in an 80% improvement in performance durability, the best among all reported bismuth-based fuel cells.
Recent Progress in Gene Therapy for Cardiovascular Disease. (2002)

Gene therapy is emerging as a potential strategy for the treatment of cardiovascular diseases, such as peripheral arterial disease, ischemic heart disease, restenosis after angioplasty, vascular bypass graft occlusion and transplant coronary vasculopathy, for which no known effective therapy exists. The first human trial in cardiovascular disease started in 1994 treating peripheral vascular disease with vascular endothelial growth factor (VEGF) and since then, many different potent angiogenic growth factors have been tested in clinical trials for the treatment of peripheral arterial disease. In addition, therapeutic angiogenesis using the VEGF gene has been used to treat ischemic heart disease since 1997. The results from these clinical trials have exceeded expectations; improvement in the clinical symptoms of peripheral arterial disease and ischemic heart disease has been reported. Another strategy for combating the disease processes, targeting the transcriptional process, has been tested in a human trial. IN particular, transfection of cis-element double-stranded (ds) oligodeoxynucleotides (ODN) (= decoy) is a powerful tool in a new class of anti-gene strategies. Transfection of ds-ODN corresponding to the cis sequence will attenuate the authentic cis-trans interaction, leading to removal of trans-factors from the endogenous cis-elements and subsequent modulation of gene expression. Genetically modified vein grafts transfected with a decoy against E2F, an essential transcription factor in cell cycle progression, appear to have long-term potency in human patients. There is great potential in gene therapy for cardiovascular disease.
Implementation of Patient-Reported Outcome Measures in U.S. Total Joint Replacement Registries: Rationale, Status, and Plans (2014)

The current stage of implementation of patient-reported outcome measures varies widely among U.S. registries. Nonetheless, evidence from the Function and Outcomes Research for Comparative Effectiveness in Total Joint Replacement registry supports the feasibility of successful implementation of patient-reported outcome measures with careful attention to the selection of the outcome measure, mode and timing of postoperative administration, and minimization of any burden on the patient and surgeon.
Synthesis of 1,6-diazaphenalene (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of 1,6-diazaphenaleneJ. C. Chang, M. El-Sheikh, A. Harmon, K. Avasthi, and J. M. CookCite this: J. Org. Chem. 1981, 46, 21, 4188–4193Publication Date (Print):October 1, 1981Publication History Published online1 May 2002Published inissue 1 October 1981https://pubs.acs.org/doi/10.1021/jo00334a016https://doi.org/10.1021/jo00334a016research-articleACS PublicationsRequest reuse permissionsArticle Views246Altmetric-Citations11LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The Development of New&amp;Existing Cabling Techniques to Obtain Realtime Full Water Column Current Measurements. (2006)

This paper provides details of the development of new and existing techniques for real-time transmission of ADCP data via strain bearing data cables in water depths exceeding 6,000 ft. The process of deploying several ADCPs different locations in the water column to obtain a full profile of the currents through the whole water column is a simple concept, however, reliable transmission of the data via a hardwired link with complete redundancy requires complex design to satisfy actual requirements. The requirements of the system include location of two ADCPs to avoid not just physical interference but also acoustic interference, one on the seabed and one at 500 m above seabed with 100% data and power redundancy using an existing 5,000 m of communications infrastructure and a limited number of copper lines. Furthermore, the requirements for a realtime link between the seabed frame and a buoy mounted ADCP utilizing a 500 m strain bearing armored data cable and the challenges of the associated weight and buoyancy requirements are discussed. Both systems provide realtime data to the offshore platform and are deployed and retrieved via ROV, as such both systems have underwater wet-mateable electrical and fiber optic connections. The challenges created by these requirements and constraints and solutions developed are presented in this paper. Many of the techniques applied in this application would be equally applicable to larger scale cabled observing systems
Kelvin probe microscopic visualization of charge storage at polystyrene interfaces with pentacene and gold (2012)

Charge carriers trapped in polystyrene (PS) were investigated with Kelvin probe microscopy (KPM) and thermally stimulated discharge current (TSDC). Lateral heterojunctions of pentacene/PS were scanned using KPM, effectively observing polarization along a side view of a lateral nonvolatile organic field-effect transistor dielectric interface. TSDC was used to observe charge migration out of PS films and to estimate the trap energy level inside the PS, using the initial rise method.
PAK4 suppresses RELB to prevent senescence-like growth arrest in breast cancer (2019)

Overcoming cellular growth restriction, including the evasion of cellular senescence, is a hallmark of cancer. We report that PAK4 is overexpressed in all human breast cancer subtypes and associated with poor patient outcome. In mice, MMTV-PAK4 overexpression promotes spontaneous mammary cancer, while PAK4 gene depletion delays MMTV-PyMT driven tumors. Importantly, PAK4 prevents senescence-like growth arrest in breast cancer cells in vitro, in vivo and ex vivo, but is not needed in non-immortalized cells, while PAK4 overexpression in untransformed human mammary epithelial cells abrogates H-RAS-V12-induced senescence. Mechanistically, a PAK4 - RELB - C/EBPβ axis controls the senescence-like growth arrest and a PAK4 phosphorylation residue (RELB-Ser151) is critical for RELB-DNA interaction, transcriptional activity and expression of the senescence regulator C/EBPβ. These findings establish PAK4 as a promoter of breast cancer that can overcome oncogene-induced senescence and reveal a selective vulnerability of cancer to PAK4 inhibition.