gebhart/patrepeval huggingface dataset:
Age- and Hypertension-Associated Protein Aggregates in Mouse Heart Have Similar Proteomic Profiles (2016)

Neurodegenerative diseases are largely defined by protein aggregates in affected tissues. Aggregates contain some shared components as well as proteins thought to be specific for each disease. Aggregation has not previously been reported in the normal, aging heart or the hypertensive heart. Detergent-insoluble protein aggregates were isolated from mouse heart and characterized on 2-dimensional gels. Their levels increased markedly and significantly with aging and after sustained angiotensin II-induced hypertension. Of the aggregate components identified by high-resolution proteomics, half changed in abundance with age (392/787) or with sustained hypertension (459/824), whereas 30% (273/901) changed concordantly in both, each P<0.05. One fifth of these proteins were previously associated with age-progressive neurodegenerative or cardiovascular diseases, or both (eg, ApoE, ApoJ, ApoAIV, clusterin, complement C3, and others involved in stress-response and protein-homeostasis pathways). Because fibrosis is a characteristic of both aged and hypertensive hearts, we posited that aging of fibroblasts may contribute to the aggregates observed in cardiac tissue. Indeed, as cardiac myofibroblasts "senesced" (approached their replicative limit) in vitro, they accrued aggregates with many of the same constituent proteins observed in vivo during natural aging or sustained hypertension. In summary, we have shown for the first time that compact (detergent-insoluble) protein aggregates accumulate during natural aging, chronic hypertension, and in vitro myofibroblast senescence, sharing many common proteins. Thus, aggregates that arise from disparate causes (aging, hypertension, and replicative senescence) may have common underlying mechanisms of accrual.
Divergent susceptibilities to AAV-SaCas9-gRNA vector-mediated genome-editing in a single-cell-derived cell population (2017)

Our results showed comparable GFP knockout efficiencies (40-50%) upon a single low-dose infection. Three consecutive transductions of 25-fold higher doses of vectors showed 80% GFP knockout efficiency. To analyze the "AAV-SaCas9-resistant cell population", we sorted the residual GFP-positive cells and assessed their permissiveness to super-infection with two AAV-Cas9-GFP vectors. We found the sorted cells were significantly more resistant to the GFP knockout mediated by the same AAV vector, but not by the other GFP-targeted AAV vector. Our data therefore demonstrate highly efficient genome-editing by the AAV-SaCas9-gRNA vector system. Differential susceptibilities of single cell-derived cells to the AAV-SaCas9-gRNA-mediated genome editing may represent a formidable barrier to achieve 100% genome editing efficiency by this vector system.
ACM SIGSPATIAL GIS Cup 2012 (2012)

The 20th ACM SIGSPATIAL Conference on Advances in Geographic Information Systems (GIS) was held in November of 2012. In conjunction with this conference, we organized the conference's first competition, called the SIGSPATIAL GIS Cup 2012. The subject of the competition was map matching, which is the problem of correctly matching a sequence of noisy GPS points to roads. We describe the details of the contest, the results of the competition, and the lessons we learned in running a contest like this.
Design of Unintuitive Antenna Geometries Using Additive Manufacturing Techniques (2020)

Using 3D printing, it is possible to create antenna geometries which are not practically feasible through traditional subtractive manufacturing techniques. There are numerous possible ways to design these geometries, such as body of revolution or extrusion methods. With the aid of multiobjective optimization, these structures can be designed for various goals. Examples include wide bandwidth, high gain, strong circular polarization, and compact size. Additionally, 3D printed antennas can offer reduced cost and weight without compromising these performance goals.
Electroreduction of Oxygen with Brominated Metallo-Corroles (2015)

Fuel cell technology raised new interest over the past two decades due to the increasing oil prices, non-sustainability of fossil fuel and the consequences of combustion on air pollution and climate changes. Two aspects limit the practicality and energy conversion efficiency in polymer electrolyte membrane fuel cells: the rate-limiting oxygen reduction reaction at the cathode and the low abundance and high cost of platinum, which still remains the most efficient catalyst. Significant effort has been made to develop alternative non-precious metal catalysts (NPMCs) for oxygen reduction. Many of those catalysts have been inspired by biological processes in that their active centers consist of transition metal complexes such as metallo-porphyrins. So far, studies conducted with such catalysts show that their activity and stability is lower than that of the state-of-the-art platinum based catalysts. In most recent years a new class of NPMCs has shown great promise; the metallo-corroles. A series of first row transition metal β-pyrrole-brominated 5,10,15-tris(penta-fluorophenyl)- Corroles [M(tpfc)Br 8, M=Mn, Fe, Co, Ni and Cu] were synthe-sized and tested as catalysts for oxygen reduction in acidic aqueous solutions. Both the reduced form and the oxidized forms of these corroles were chara-cterizes by means of NMR, EPR and UV-vis measurements. Rotating Ring-Disk electrode (RRDE) was used to test the kinetic performance of these corroles in the catalytic reaction of oxygen reduction (ORR). The corroles were adsorbed on a high surface area carbon powder (BP2000) prior to electro-chemical measurements, to create a unique composite material. The comparison between the corroles with different metal centers showed a favorable catalytic performance of the ORR in the case of the Co-Corrole, which showed an onset potential of only 0.8V vs . RHE (Figure 1). Figure 1
La chimie et la santé (2020)

Non, tout n'oppose pas chimie et santé ! Si l'on ne devait écouter que l'opinion publique manipulée par les médias, la première est la responsable des « bienfaits » de la pollution tandis que la seconde est la valeur suprême de nos sociétés, à laquelle tout le monde devrait avoir droit. Autant le dire tout de suite, si l'une est si chère à notre société, sans l'autre rien de tout cela ne serait possible. En effet, sans les diagnostics et les médicaments, les progrès de la médecine auraient été moins flagrants. S'il démonte d'abord ce paradoxe en dénonçant ce manque de rigueur qui provoque tant d'idées fausses sur les dangers des molécules de notre environnement, ce livre, qui s'adresse aux non-spécialistes, veut surtout nous faire saisir la révolution médicale en cours : allongement de la vie, recul des maladies les plus graves, combat contre la douleur…, représentant un programme de décennies de recherches. Et ce qu'on a découvert, c'est une formidable illustration du fameux « tout est chimie » des savants anciens qu'aimait à citer le chimiste et pharmacien Pierre Potier. Chimie, c'est d'abord la composition du vivant dont l'étude à l'échelle moléculaire dévoile toutes ces molécules de base, protéines, acides nucléiques, assemblages qui ne cessent de nous étonner par leur diversité. Chimie, c'est aussi le fonctionnement de tout ce système moléculaire, les interactions subtiles entre ses différentes composantes auxquelles on rattache progressivement toutes les fonctions de la vie. Mais chimie, c'est encore beaucoup plus : c'est le pouvoir de l'homme – biologiste ou médecin – d'agir de façon raisonnée sur ce monde vivant que la chimie lui fait découvrir. On le comprend : on est passé du souci de compréhension du vivant à une stratégie pour soigner et guérir. S'il illustre les divers aspects de cette nouvelle pharmacologie, ce livre n'oublie pas que la révolution médicale, c'est aussi celle des diagnostics, de la détection précoce des dysfonctionnements. On ne peut qu'être admiratif devant la possibilité de l'imagerie – voir l'intérieur du corps – et les développements que nous promettent les laboratoires de chimie pour rendre ces techniques physiques encore plus performantes. Médicaments du futur, capacité de « voir » les organes : la chimie vient révolutionner notre pouvoir sur la maladie. Réussirait-elle à remplir notre rêve « déraisonnable » – bonne santé pour tous,toujours ? Le lecteur trouvera ici les éléments de sa réponse.
Direct photo emission monitoring for high power IGBT during avalanche operation (2016)

This paper proposes a direct photo emission monitoring during avalanche operations for power devices using a streak camera. IGBTs (Insulated Gate Bipolar Transistors) are very important power devices and IGBTs have been developed to improve trade-off relation between on-state losses and switching losses. And also a reliability enhancement of IGBTs is very important issue. To obtain reliability enhancement, it is important to investigate an avalanche phenomenon of IGBTs, directly. During avalanche operation, it is well known that visible light is emitted from the device. This is the first study to succeed in observing the photo emission directly from avalanche phenomena under UIS (Unclamped Inductive Switching) condition of IGBTs. We have also measured moving the emission region in edge termination area.
eHealth - Enhancing Mobility with Aging (2009)

Vegetable Market have become major sources of organic waste. Some of such waste when being diverted to landfills not only increase the landfill loading but also contribute to increase greenhouse gas emission. Of the many technologies available in handling such hugely generated waste, composting has proven very effective for decades. Enzyme and non-enzyme mediated aerobic composting of vegetable market complex waste (VMCW) have been investigated. Conventional composting technique though being capable of handling large quantum of waste are found to consume more time. Proven to be disadvantages factor. In the present investigation, the pre-cultured seed inoculums used for vegetable market complex waste, shortened the typical composting period from 45 to 9 days for the first time. Also, rapid size and volume reduction of VMCW was witnessed. The organic degradation of VMCW was observed as 42% (82 ± 2.83% to 40.82 ± 0.61%), with a volume reduction from 0.012m<sup>3</sup> to 0.003 m<sup>3</sup> within 9 days. An enriched nutrients NPK level of compost bio-fertilizer was recorded as 0.91% w/w, 0.5% w/w and 1.029% w/w respectively. Compost maturity observed through the X-ray diffraction (XRD) analysis of the manure confirmed the conversion of the crystal structure of the compost particle to amorphous form and the mineralization of organic matter during the composting. Thus, the fermented pre-cultured seed inoculums favored an enhanced nutrients level with shortened composting time.
Databases and Units of Measure (1982)

A serious impediment to the integrated use of databases across international boundaries, scientific disciplines and application areas is the use of different units of measure, e.g., miles or kilometers for distance, dollars or rupees for currency, and Btu or kWh for energy. Units of measure are not specified in the data definition language but associated by convention with values in the database. Inconsistent usage of values with different units cannot be checked atuomatically. Retrieval, storage, and manipulation of data values in units other than those associated with the values requires that the user perform the appropriate conversions. This process is inconvenient and error prone. These problems are illustrated by an international database example.
Application of pattern recognition techniques in ultrasound tissue characterization (2002)

An approach for the application of multivariate pattern recognition techniques to detection of diffuse and focal disease using acoustic data is reviewed. Supervised and unsupervised techniques are implemented to design the best ultrasonic tissue signature for a given task from a set of measurements. The performances of both techniques are evaluated and compared using several methods. However, it is desirable to utilize a technique that quantitatively detects and displays the heterogeneity of an ultrasound image. It is shown that, for a particular task, choosing features with physical significance will make the classification of the data more robust. It is also shown that the success of combining supervised and unsupervised techniques using such features extends beyond discrimination of one class of data from the other and that the approach can be used to grade and the variations in the same tissue type.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Abstract LB-288: CDC7 kinase inhibition by SRA141 induces a potentially novel caspase-dependent tumor cell apoptosis associated with altered DNA replication and cell cycle dynamics (2019)

DNA replication is a tightly regulated process required for faithful duplication of the genome. Previous research in lower eukaryotes has demonstrated a requisite role for the CDC7 protein kinase in DNA replication origin firing through phosphorylation of the MCM2-7 helicase complex. Owing to its overexpression in various neoplasms, CDC7 has emerged as an attractive target for cancer treatment. We previously reported that SRA141, a potent and selective CDC7 inhibitor, is cytotoxic to multiple tumor cell lines in vitro and demonstrates robust anti-tumor efficacy in colorectal and leukemia xenograft models. Here, we explored the effects of SRA141 on DNA replication and cell cycle dynamics and how these effects relate to the mechanism of SRA141-induced cell death in colorectal cancer cell lines.SRA141 treatment caused a complete inhibition of MCM2 phosphorylation and reduced overall cellular DNA synthesis within 3 hours, with the greatest effects observed in late S-phase. Unexpectedly, DNA combing experiments indicated that SRA141 caused a 70-90% increase in the rate of replication fork progression. These findings suggest that SRA141 may indeed limit origin firing, but this effect is compensated for in part by increased replication fork rates. Despite delay, nucleotide analog pulse-chase experiments indicated that SRA141 treated cells ultimately finished S phase and transitioned into mitosis. In contrast to other reports, no evidence of replication fork collapse was observed, indicating that the main mechanism of SRA141 cytotoxicity is not mediated through replication fork collapse. Intriguingly, some of the most pronounced effects of SRA141 treatment were on progression through M phase, as evidenced by an accumulation of mitotic cells and increased Cyclin B levels following treatment. Moreover, inhibition of Aurora B kinase, which regulates mitotic progression, strongly synergized with SRA141 in caspase-dependent cancer cell killing. Taken together, our findings indicate that the mechanism of cytotoxicity of CDC7 inhibitors is distinct from agents that cause replication fork collapse or inhibit CDKs, and thus may define a new class of cancer therapeutic agents that synergize with drugs that target certain mitotic pathways.Citation Format: Veena Jagannathan, Snezana Milutinovic, Ryan Hansen, Bryan Strouse, Christian Hassig, Eric Brown. CDC7 kinase inhibition by SRA141 induces a potentially novel caspase-dependent tumor cell apoptosis associated with altered DNA replication and cell cycle dynamics [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr LB-288.
Emerging Grid Networking Services and Technologies (2006)

This chapter contains sections titled: Introduction New Enabling Technologies Edge Technologies Wireless Technologies Access Technologies Core Technologies Photonic Integrated Circuits (PIC) Acknowledgments References
Data from SCH 2047069, a Novel Oral Kinesin Spindle Protein Inhibitor, Shows Single-Agent Antitumor Activity and Enhances the Efficacy of Chemotherapeutics (2023)

&lt;div&gt;Abstract&lt;p&gt;Kinesin spindle protein (KSP) is a mitotic kinesin required for the formation of the bipolar mitotic spindle, and inhibition of this motor protein results in mitotic arrest and cell death. KSP inhibitors show preclinical antitumor activity and are currently undergoing testing in clinical trials. These agents have been dosed intravenously using various dosing schedules. We sought to identify a KSP inhibitor that could be delivered orally and thus provide convenience of dosing as well as the ability to achieve more continuous exposure via the use of dose-dense administration. We discovered SCH 2047069, a potent KSP inhibitor with oral bioavailability across species and the ability to cross the blood-brain barrier. The compound induces mitotic arrest characterized by a monaster spindle and is associated with an increase in histone H3 and mitotic protein monoclonal 2 phosphorylation both &lt;i&gt;in vitro&lt;/i&gt; and &lt;i&gt;in vivo&lt;/i&gt;. SCH 2047069 showed antitumor activity in a variety of preclinical models as a single agent and in combination with paclitaxel, gemcitabine, or vincristine. Mol Cancer Ther; 9(11); 2993–3002. ©2010 AACR.&lt;/p&gt;&lt;/div&gt;
Development of Two Multiplexed Immunoassays for Study of Blood-Based Biomarkers of Alzheimer's Disease (P04.224) (2013)

OBJECTIVE: To develop multiplexed immunoassays that measure candidate biomarkers for Alzheimer's disease (AD) in blood.
A synthetic strategy for mimicking the extracellular matrix provides new insight about tumor cell migration (2009)

Understanding the role of the tumor microenvironment during cancer progression and metastasis is complicated by interactions between cells, the extracellular matrix (ECM), and a variety of biomolecules. Using a synthetic strategy, we investigated proteolytic modes of migration for HT-1080 fibrosarcoma cells in an environment that limited confounding extracellular influences. A large percentage of HT-1080s migrated through a Rho kinase (ROCK)-dependent rounded morphology with a leading edge protrusion that defined the direction of migration, and migration was only weakly dependent on the adhesive peptide RGDS. HT-1080s migrating in thiol-ene hydrogels are more rounded and exhibit much more invasive behavior than dermal fibroblasts. Our results indicate that HT-1080s have the capacity to migrate through a mechanism that is distinct from mesenchymal cells, with significant amoeboid character even when utilizing a proteolytic migration strategy. The migration mode observed here provides insight into the invasiveness of metastatic cells in vivo and demonstrates the potential of a synthetic strategy for investigating complex biological problems.
TH-AB-204-05: Real-Time Prevention of Radiation ‘mistakes’ Using Cherenkov Light Emission (2015)

Purpose: To determine the feasibility of using real-time imaging of Cherenkov light emission from an object's surface for the qualitative identification of radiation ‘mistakes’. Methods: A highly sensitive camera was used to image the Cherenkov light emitted for a solid water phantom. A Varian 2100CD was used to deliver 500 MU for a 6 MV photon beam operating a dose rate of 600 MU/min, and the chosen imaging system and geometry provided data with a field of view of 30×30 cm, at 10 FPS, at a resolution of approximately 0.6 mm. Three mistake scenarios were imaged, two static errors (1) incorrect jaw size for stereotactic delivery using a 20 mm cone, (2) incorrect wedge angle applicator for a 10×10 cm field, and a dynamic errors (3) MLC leaf error and (4) incorrect collimator or gantry angle for IMRT delivery. Results: Using the real-time results of Cherenkov light emission imaging, the incorrect jaw size setting for SRS or SRT and incorrect wedge angle applicator were clearly identified in the first captured image. For the detection of a MLC leaf error, the proposed method was able to provide 4 frames per field, and detect leaf errors down to 1 mm and collimator and gantry errors down to 1 deg. Discussion and Conclusions: While the results in this feasibility study were performed using a solid water phantom, the technique could be readily applied in-vivo, as Cherenkov light emission imaging is completely non-invasive and requires no external materials to be introduced into the Linac or radiation beam path. For the detection of static errors, by providing real-time information after a single MU of delivery, further incorrect radiation field delivery could be avoided. Furthermore, dynamic errors in leaf positioning collimator or gantry angle could be detected in real-time down to 1 mm or 1 deg.
Electrochemical Interface Doping in Organic Light Emitting Field Effect Transistors (2005)

Electrochemical interface doping is introduced as a new concept for the realization of organic light‐emitting field‐effect transistors (O‐LEFTs). In a bottom contact tetracene transistor a 2–4 nm poly(ethylene‐oxide) layer with lithium triflate was inserted as interface layer between the gate‐oxide and the tetracene layer. For the first time a polymeric electrolyte is combined with “small molecule” functional material. The injection of both, holes and electrons allows for radiative recombination in the transistor channel. The onset of light emission occurs at a source‐drain‐voltage below 10 V and can be controlled by the gate voltage.
Subthreshold-current reduction circuits for multi-gigabit DRAM's (1994)

Two subthreshold-current reduction circuit schemes are described to suppress the increase in current in multi-gigabit DRAM's. One is a hierarchical power-line scheme for iterative circuits. In this scheme, a group of circuits is divided into blocks; only the selected block is supplied with power, while the subthreshold current to the many nonselected blocks is reduced. This scheme minimizes the number of circuits carrying the large subthreshold current. Applications of this scheme to word drivers, decoders and sense-amplifier driving circuits are shown. The other scheme is a switched-power-supply inverter with a level holder for random combinational logic circuits. In the active mode of the chip, the operating period of the inverter is distinguished from the inactive period. The inverter is supplied with power only in the operating period, while in the inactive period the subthreshold current is shut off and the output level is kept by the flip-flop level holder. This scheme shortens the period in which the large subthreshold current flows. Both schemes are evaluated for a conceptually-designed 16-Gb DRAM. They reduce its active current by ten-fold from the conventional 1.2 A to 116 mA.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>