gebhart/patrepeval huggingface dataset:
The Role of Cilostazol, a Phosphodiesterase 3 Inhibitor, on Oocyte Maturation and Subsequent Pregnancy in Mice (2012)

It is important to identify effective contraceptive drugs that cause minimal disruption to physiological processes. Phosphodiesterase 3 (PDE3) inhibitors suppress meiosis in oocytes by decreasing the level of cAMP and blocking the extrusion of the first polar body. In this study, we tested the PDE3 inhibitor, cilostazol, as a potential contraceptive agent. The effects of cilostazol treatment in vitro and in vivo on the suppression of oocyte maturation in a mouse model were investigated. The results indicated that treatment with increasing concentrations of cilostazol led to a dose-dependent arrest in meiosis progression. The effective in vitro concentration was 1 µM and was 300 mg/kg in vivo. The effect of cilostazol was reversible. After removal of the drug, meiosis resumed and mouse oocytes matured in vitro, and showed normal chromosome alignment and spindle organization. After fertilization using an ICSI method, the oocytes showed normal morphology, fertilization rate, embryo cleavage, blastocyst formation, and number of viable pups when compared with controls. The offspring showed similar body weight and fertility. In vivo, the mice became infertile if the drug was injected sequentially, and became pregnant following discontinuation of cilostazol. More importantly, no side effects of cilostazol were observed in treated female mice as demonstrated by blood pressure and heart rate monitoring. It is concluded that cilostazol, a drug routinely used for intermittent claudication, can effectively inhibit oocyte maturation in vitro and in vivo, does not affect the developmental potential of oocytes following drug removal and has few side effects in female mice treated with this drug. These findings suggest that cilostazol may be a potential new contraceptive agent that may facilitate an efficacy and safety study of this drug.
NCHRP Report 350 Testing and Evaluation of NETC Bridge Rail Transitions (2006)

In two previous projects, the New England Transportation Consortium (NETC) developed and crash tested two steel bridge rail systems - a 2-Bar Curb-Mounted system and a 4-Bar Sidewalk-Mounted system. The NETC 2-Bar Curb-Mounted Bridge Rail was tested to Performance Level 2 (PL-2) in accordance with the American Association of State Highway and Transportation Officials (AASHTO) Guide Specifications for Bridge Railings and was effectively accepted as a National Cooperative Highway Research Program (NCHRP) Report 350 Test Level 4 (TL-4) railing by Federal Highway Administration (FHWA) Office of Highway Safety. The NETC 4-Bar Sidewalk-Mounted Bridge Rail met evaluation criteria for an NCHRP Report 350 traffic barrier at TL-4. The FHWA Office of Highway Safety Infrastructure has concluded that both bridge rails are acceptable as TL-4 designs and may be used on the National Highway System. The objective of the proposed research study was to qualify transitions to the two bridge rail systems and to a concrete end wall that is used as a component of several bridge rail systems. The NETC New Hampshire transition to the two-bar steel bridge rail successfully contained and redirected the pickup for TL-3 test conditions. All occupant risk values were acceptable. The NETC Massachusetts transition to the concrete end wall successfully contained and redirected both the pickup and the single unit truck for TL-4 test conditions. All occupant risk values were acceptable where applicable. This report presents the details of each installation tested, a description of the testing, and assessment of the tests.
Subwavelength engineered fiber-to-chip silicon-on-sapphire interconnects for mid-infrared applications (Conference Presentation) (2016)

The mid-Infrared wavelength range (2-20 µm), so-called fingerprint region, contains the very sharp vibrational and rotational resonances of many chemical and biological substances. Thereby, on-chip absorption-spectrometry-based sensors operating in the mid-Infrared (mid-IR) have the potential to perform high-precision, label-free, real-time detection of multiple target molecules within a single sensor, which makes them an ideal technology for the implementation of lab-on-a-chip devices. Benefiting from the great development realized in the telecom field, silicon photonics is poised to deliver ultra-compact efficient and cost-effective devices fabricated at mass scale. In addition, Si is transparent up to 8 µm wavelength, making it an ideal material for the implementation of high-performance mid-IR photonic circuits. The silicon-on-insulator (SOI) technology, typically used in telecom applications, relies on silicon dioxide as bottom insulator. Unfortunately, silicon dioxide absorbs light beyond 3.6 µm, limiting the usability range of the SOI platform for the mid-IR. Silicon-on-sapphire (SOS) has been proposed as an alternative solution that extends the operability region up to 6 µm (sapphire absorption), while providing a high-index contrast. In this context, surface grating couplers have been proved as an efficient means of injecting and extracting light from mid-IR SOS circuits that obviate the need of cleaving sapphire. However, grating couplers typically have a reduced bandwidth, compared with facet coupling solutions such as inverse or sub-wavelength tapers. This feature limits their feasibility for absorption spectroscopy applications that may require monitoring wide wavelength ranges. Interestingly, sub-wavelength engineering can be used to substantially improve grating coupler bandwidth, as demonstrated in devices operating at telecom wavelengths. Here, we report on the development of fiber-to-chip interconnects to ZrF4 optical fibers and integrated SOS circuits with 500 nm thick Si, operating around 3.8 µm wavelength. Results on facet coupling and sub-wavelength engineered grating coupler solutions in the mid-IR regime will be compared.
Polymeeroppervlakken : structuur en dynamica van polymeeroppervlakken en -grenslagen (1997)

No abstract.
Functional analysis of a T cell line specific for antiidiotypic antibodies to a Schistosoma mansoni protective epitope. II. Induction of protective immunity in experimental rat schistosomiasis (1991)

In our previous work on the idiotypic network in the rat model of schistosomiasis we showed that immunization with an IgE mAb specific for 26/56-kDa parasitic Ag resulted in the production of anti-anti-Id antibodies of both the IgG and IgE classes. Further studies demonstrated that anti-Ab2 T cell lines, obtained by immunization with Ab2 antibodies, functioned as conventional Th cells; they were MHC-restricted and required APC to proliferate in the presence of the native schistosomula Ag and the Ab2 antibodies. We report the involvement of these anti-Ab2 cells in the regulation of protective immunity. The transfer of long term culture anti-Ab2 T cell lines into LOU/M rats, followed by a challenge infection by Schistosoma mansoni 1 day after the cell transfer led to a slight increase in the worm burden. On the contrary, the transfer of anti-Ab2 T cells 90 days before S. mansoni infection induced a significant reduction of the worm burden (up to 57%). T cells recovered from the protected rats were stimulated by the native schistosomula Ag as well as by tryptic fragments of IgG isolated from the Ab2 sera, in the presence of irradiated thymic cells as APC. We also analyzed the humoral response developed by the rats after transfer with the anti-Ab2 T cell lines. The sera induced various inflammatory cells into cytotoxic effectors against the larvae of S. mansoni, arguing for the presence of functional IgE in the sera. Moreover, when these sera were passively transferred into rats infected 1 day later, a significant reduction of the worm burden was observed. However, antibody-dependent cytotoxic mechanisms efficient 10 days after the anti-Ab2 T cell transfer did not correlate with the protective immunity which required a 90-day delay to be established. These data suggest that the protective immunity induced by the anti-Ab2 cells is supported both by the cellular and humoral components and that in a future vaccinating strategy the idiotypic network may play a crucial role.
Crystal Structure of a Metal Ion-bound IS200 Transposase (2005)

IS200 transposases, present in many bacteria and Archaea, appear to be distinct from other groups of transposases. To provide a structural basis for understanding the action of IS200 transposases, we have determined the crystal structure of the SSO1474 protein from Sulfolobus solfataricus, a member of the IS200 family, in both Mn(2+)-bound and Mn(2+)-free forms. Its monomer fold is distinct from other classes of structurally characterized transposases. Two monomers form a tight dimer by exchanging the C-terminal alpha-helix and by merging the two central beta-sheets into a large beta-sheet. Glu(55), His(62), and four water molecules provide the direct coordination sphere of the catalytically essential metal ion in the Mn(2+)-bound structure. His(16), Asp(59), and His(60) also play important roles in maintaining the metal binding site. The catalytic site is formed at the interface between monomers. The candidate nucleophile in the transposition mechanism, strictly conserved Tyr(121) coming from the other monomer, is turned away from the active site, suggesting that a conformational change is likely to occur during the catalytic cycle.
Interaction of Triarylpyrylium Salts with Anhydrides of Dicarboxylic Acids (1995)

Activation of reactivity of pyrylium salts by means of salts of carboxylic acids or tertiary amines makes possible the interaction of pyrylium salts with weak CH acids, such as anhydrides of dicarboxylic acids.
SDH Subunit Mutation Status in Saliva: Genetic Testing in Patients with Pheochromocytoma (2016)

Germline mutations occur in up to 30-40% of pheochromocytoma/paraganglioma, with mutations in the succinate dehydrogenase (SDH) subunits B (SDHB) and D (SDHD) being the most common. Blood samples are favored for obtaining high quality DNA, however, leukocytes can also be obtained by collecting saliva. The aim of this study was to determine whether SDHB and SDHD gene mutations in patients with pheochromocytoma/paraganglioma could be determined using a salivary sample. Paired blood and salivary samples were collected from 30 patients: 9 SDHB mutation positive, 13 with a SDHD mutation, and 8 without any SDHx mutations. The Oragene DISCOVER kit was used to collect and extract DNA from saliva. Blood DNA was extracted from EDTA blood samples. The DNA purification and concentration were measured by spectrophotometry. The 8 exons of SDHB and the 4 exons of SDHD were amplified and sequenced by PCR-based bidirectional Sanger sequencing. Total DNA yields from blood DNA were similar to those obtained from saliva DNA [mean (±SD) saliva vs. blood DNA concentration 514.6 (±580.8) ng/µl vs. 360.9 (±262.7) ng/µl; p=0.2)]. The purity of the saliva DNA samples was lower than that of blood [mean OD260/OD280 ratio 1.78 (±0.13) vs. 1.87 (±0.04); p=0.001, respectively], indicating more protein contamination in the saliva-extracted DNA. This study shows that salivary DNA collected from patients with pheochromocytoma/paraganglioma is a good alternative for extraction of genomic DNA for its high DNA concentration and acceptable purity and can be used as an alternative to blood derived DNA in screening for SDHB and SDHD mutations.
<title>STARNET-E: a broadband wavelength-division multiplexed optical local-area network</title> (1992)

We propose enhancements to STARNET, a previously proposed wavelength division multiplexed broadband optical local area network. STARNET offers the users both a medium- speed packet-switched ring network, and a high-speed circuit interconnection. The enhanced architecture, STARNET-E, improves performance in both the packet and the circuit interconnect sections over the previous version. Performance analysis of the STARNET-E packet network shows that this data transport facility can be upgraded to Gb/s total throughput with little added complexity with respect to the original STARNET. Moreover, we show that STARNET nodes can be enhanced to allow reconfigurable multihop topologies with little added optical hardware. The throughput of multihop distributed switching over a passive star physical topology in STARNET-E is compared to the throughput of an active centralized switch. STARNET-E offers to all nodes a Gb/s total throughput packet transport facility and a high speed circuit interconnect, simultaneously and independently. In addition, a node can trade the high speed circuit interconnect service for a multihop broadband packet network without the need for any added optical hardware.
A series of case studies illustrating the role of flow cytometry in the diagnostic work‐up of myelodysplastic syndromes (2022)

Current guidelines recommend flow cytometric analysis as part of the diagnostic assessment of patients with cytopenia suspected for myelodysplastic syndrome. Herein we describe the complete work-up of six cases using multimodal integrated diagnostics. Flow cytometry assessments are illustrated by plots from conventional and more recent analysis tools. The cases demonstrate the added value of flow cytometry in case of hypocellular, poor quality, or ambiguous bone marrow cytomorphology. Moreover, they demonstrate how immunophenotyping results support clinical decision-making in inconclusive and clinically 'difficult' cases.
Lewis acid promoted reactions of n-(1-phenylcyclopropyl)alkanoyl chlorides. Ring-size effects in competitive intramolecular acylation of phenyl and cyclopropyl substituents (1978)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLewis acid promoted reactions of n-(1-phenylcyclopropyl)alkanoyl chlorides. Ring-size effects in competitive intramolecular acylation of phenyl and cyclopropyl substituentsMichael P. Doyle, Robert C. Elliott, and Joseph F. Dellaria Jr.Cite this: J. Org. Chem. 1978, 43, 23, 4459–4461Publication Date (Print):November 1, 1978Publication History Published online1 May 2002Published inissue 1 November 1978https://pubs.acs.org/doi/10.1021/jo00417a013https://doi.org/10.1021/jo00417a013research-articleACS PublicationsRequest reuse permissionsArticle Views108Altmetric-Citations6LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Molecular Identification of Western European Species of Obsoletus Complex (Diptera: Ceratopogonidae) by an Internal Transcribed Spacer-1 rDNA Multiplex Polymerase Chain Reaction Assay (2007)

In southern Europe, orbiviral diseases such as bluetongue (BT) have been assumed to have been largely transmitted by the classical Afro-Asian vector Culicoides imicola Kieffer (Diptera: Ceratopogonidae). Recent outbreaks have occurred in regions where C. imicola is normally absent, supporting the theory that other species belonging to the Obsoletus or Pulicaris complexes may play a role in BT virus transmission. Investigations of the ecology of the species within the former group are hampered by females of member species being extremely difficult to separate by classical morphology. To allow straightforward separation of these species in France, a multiplex polymerase chain reaction-based on internal transcribed spacer (ITS)-1 rDNA was developed to distinguish between Culicoides chiopterus Meigen, Culicoides dewulfi Goetghebuer, Culicoides montanus Shakirjanova, Culicoides obsoletus Meigen, and Culicoides scoticus Downes &amp; Kettle. This tool will be useful in defining both the vector role and larval biotopes of these species in Europe.
Cloning and Expression of the Sarcosine Oxidase Gene fromBacillussp. NS-129 inEscherichia coli (1991)

The gene coding for a thermostable sarcosine oxidase (EC 1.5.3.1) was isolated from Bacillus sp. NS-129. The primary structure of sarcosine oxidase deduced from the nucleotide sequence was a protein composed of 387 amino acids with molecular weight 42,955. When the sarcosine oxidase was overproduced to about 35% of soluble protein in E. coli under the control of a lac promoter, the sarcosine oxidase activity of the crude extract was increased 3-fold by the addition of FAD. This indicates that most of the enzyme is accumulated in an inactive form, a flavinless aporotein, in the cell.
DEVELOPMENT OF DRAINAGE COEFFICIENTS AND LOSS OF SUPPORT VALUES FOR PAVEMENT DESIGN IN NEBRASKA (1998)

A chart of drainage time to achieve 50% saturation for bases and subbases with edge drains was developed. Using this chart recommended values for drainage coefficients for portland cement concrete (PCC) and asphalt concrete (AC) pavements can be determined from the 1993 American Association of State Highway and Transportation Officials (AASHTO) Design Guide. At the sites evaluated, pavement drainage in Nebraska is rated Poor to Very Poor. Therefore, the drainage coefficient will range from 0.95 to 0.70 depending on topography of the right-of-way and climate. Loss of Support values of 1 to 1.5 are appropriate for design, unless highly permeable non-erodable subbases are designed so that pavement drainage can be rated Good. A computer model which incorporates the AASHTO 1993 design equation for PCC pavement is presented in a spreadsheet format that provides ease of design for evaluation of alternate criteria and material properties. Two design examples representing conditions at one of the test sites are presented. The examples assume poor and good drainage for design assumptions comparison.
Integration of “Omics” Data with Genome‐Scale Metabolic Models (2012)

This chapter contains sections titled: Introduction Genome-Scale Metabolic Networks Constraint-Based Modeling Theory Current Analysis of Omics Data New Approaches to Developing Model Constraints Use of Gene Expression Data in Metabolic Models Use of Metabolomics Data in Metabolic Models: TMFA Example Integration of Multiple Omics Data Sets Future Directions and Applications to Strain Engineering References
Osmotic pressure of star and ring polymers in semidilute solution (1986)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOsmotic pressure of star and ring polymers in semidilute solutionBinny J. Cherayil, M. G. Bawendi, Akira Miyake, and Karl F. FreedCite this: Macromolecules 1986, 19, 11, 2770–2778Publication Date (Print):November 1, 1986Publication History Published online1 May 2002Published inissue 1 November 1986https://pubs.acs.org/doi/10.1021/ma00165a019https://doi.org/10.1021/ma00165a019research-articleACS PublicationsRequest reuse permissionsArticle Views202Altmetric-Citations13LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The cambridge university 2014 BOLT conversational telephone Mandarin Chinese LVCSR system for speech translation (2015)

This paper presents the development of the 2014 Cambridge University conversational telephone Mandarin Chinese LVCSR system for the DARPA BOLT speech translation evaluation. A range of advanced modelling techniques were employed to both improve the recognition performance and provide a suitable integration with the translation system. These include an improved system combination technique using frame level acoustic model combination via joint decoding. Sequence trained deep neural network (DNN) based hybrid and tandem systems were combined on-the-fly to produce a consistent decoding output during search. A multi-level paraphrastic recurrent neural network LM (RNNLM) modelling both alternative paraphrase expressions and character sequences while preserving a consistent character to word segmentation was also used. This system gave an overall character error rate (CER) of 29.1% on the BOLT dev14 development set. Index Terms: conversational speech transcription, speech translation, system combination, RNNLM, character LM
An Egg-Derived Sulfated N -Acetyllactosamine Glycan Is an Antigenic Decoy of Influenza Virus Vaccines (2021)

Influenza viruses grown in eggs for the purposes of vaccine generation often acquire mutations during egg adaptation or possess different glycosylation patterns than viruses circulating among humans. Here, we report that seasonal influenza virus vaccines possess an egg-derived glycan that is an antigenic decoy, with egg-binding MAbs reacting with a sulfated <i>N</i>-acetyllactosamine (LacNAc). Half of subjects that received an egg-grown vaccine mounted an antibody response against this egg-derived antigen. Egg-binding monoclonal antibodies specifically bind viruses grown in eggs, but not viruses grown in other chicken-derived cells, suggesting that only egg-grown vaccines can induce antiegg antibodies. Notably, antibodies against the egg antigen utilized a restricted antibody repertoire and possessed features of natural antibodies, as most antibodies were IgM and had a simple heavy-chain complementarity-determining region 3. By analyzing a public data set of influenza virus vaccine-induced plasmablasts, we discovered egg-binding public clonotypes that were shared across studies. Together, this study shows that egg-grown vaccines can induce antibodies against an egg-associated glycan, which may divert the host immune response away from protective epitopes.
Stacked Pt/SrBi2Ta2-xNbxO9/Pt/IrOx/Ir Capacitor on Poly Plug (2002)

A Pt/SrBi2Ta2-xNbxO9(SBTN)/Pt/IrOx/Ir capacitor was successfully fabricated up to the stage of metal-1 etching process on a polysilicon plug for mega-bit ferroelectric random access memory. The integration processes include the chemical-mechanical polishing technique, buried TiN barrier structure and electrode technologies for high thermal stability, and a low-temperature process for SBTN film. The thickness of the iridium layer was the most important factor in controlling the contact resistance of the plug. The Pt thickness also affected the contact resistance of the plug. The best contact resistance of the plug was about 2.0 kΩ/plug at the maximum process temperature of 750°C for 3 min in oxygen ambient at the contact size of φ0.30 µm. Hysteresis curves of the SBTN capacitor were obtained after the metal-1 etching process. The capacitor size dependency of the polarization was not observed in the range of 0.30–25 µm2 and the values of the sensing polarization were about 10 µC/cm2 at the applied voltage of 3 V. In addition, the capacitor exhibited no fatigue loss up to 5×1010 cycles at the switching voltage of 3 V.