gebhart/patrepeval huggingface dataset:
Dysregulation of HOX11 by Chromosome Translocations in T-cell Acute Lymphoblastic Leukemia: A Paradigm for Homeobox Gene Involvement in Human Cancer (1995)

The translocation t(10;14)(q24;q11) is observed in the course of routine cancer cytogenetic studies in 5-10% of patients with T-cell acute lymphoblastic leukemia (ALL). Recent molecular dissections of t(10;14) translocations support the hypothesis that these relatively gross chromosomal mutations represent key genetic steps in neoplastic transformation. The genes consistently involved are the T-cell receptor (TCR) delta-chain gene in 14q11 and a human homeobox-containing gene in 10q24, HOX11, initially identified through cloning of t(10;14) translocations. Like other homeoproteins, HOX11 binds DNA with sequence specificity and is likely to be a transcription factor, controlling the expression of developmentally important genes. The t(10;14) translocations arise as a result of aberrant physiological recombinational events that occur at early stages of T-cell development, probably during failed attempts at TCR gene rearrangement. The net result of the aberrant genetic recombinations is inappropriate expression of HOX11 in individual T-cells that acquire the mutation. Tlx-1, the murine homolog of HOX11, is expressed embryologically in the developing spleen and in structures derived from cranial neural crest cells and migratory paraxial mesoderm. Mice homozygously deleted for Tlx-1 are asplenic. Thus, HOX11 may be one of the first examples in mammals of a "master gene" acting as a regulatory switch controlling a downstream program of organ-specific cell growth and proliferation. Preliminary tumorigenicity assays suggest that HOX11 expression in hematopoietic cells most likely plays an immortalization role in neoplastic transformation.(ABSTRACT TRUNCATED AT 250 WORDS)
Silsesquioxane-bonded zirconocene complexes as soluble models for silica-tethered olefin polymerization catalysts (2003)

Silsesquioxane tethered fluorene ligands [R7Si8O12X]-9-Flu(H)(R =c-C6H11, X =–( 1); R =c-C5H9, X = CH2( 2), (CH2)3( 3), C6H4CH2( 4)), (c-C5H9)7Si8O12CH2-9-Flu(9-EMe3)(E = Si ( 5a), Sn ( 5b)) and zirconium dichlorides Cp[(c-C5H9)7Si8O12CH2-9-Flu]ZrCl2( 6b, Cp= 1,3-C5H3(SiMe3)2), Cp*[(c-C5H9)7Si8O12-X-9-Flu]ZrCl2(X = CH2( 6a), (CH2)3( 7), C6H4CH2( 8)) and [(c-C5H9)7Si8O12CH2-9-Flu]2ZrCl2( 9) have been applied as models for silica-tethered ancillary ligands and silica tethered-zirconocenes. Immobilization of zirconocenes containing a pendant anchorable functionality, Cp[Me2(EtO)SiCH2Flu]ZrCl2( 10) and Cp[C5Me4SiMe2OEt]ZrCl2( 11) was considerably hampered by competitive Zr–Cl and Si–OEt bond splitting. When activated with MAO (methylalumoxane), 6–9 yield active ethylene polymerization systems.
Abstract 260: Nanoparticle-mediated Simultaneous Targeting to Mitochondria and Inflammatory Monocytes Confers Additive Cardioprotection Against Myocardial Ischemia-reperfusion Injury (2015)

Background: Targeting one mediator of myocardial ischemia-reperfusion (IR) injury failed to reduce infarct size in clinical trials, suggesting the necessity of the innovative strategy to target more than 2 mediators simultaneously. Previously, we have engineered poly(lactic acid/glycolic acid) nanoparticle containing cyclosporine A (CsA-NP) and pitavastatin (Pitava-NP), and reported that the former inhibits the opening of mitochondrial permeability transition pore (mPTP) and the latter reduces monocyte-mediated inflammation in IR hearts. Here we tested the hypothesis that nanoparticle-mediated simultaneous targeting to mPTP and monocytes confers additive cardioprotection against IR injury. Methods and Results: We produced mice deficient with both cyclophilin D (CypD, a key molecule for mPTP opening) and CCR2 (a receptor for monocyte chemoattractant protein-1), and found that the double-KO mice displayed dramatic reduction in myocardial IR injury model (Fig. A). Flow cytometric analysis and fluorescence molecular tomography showed that inflammation was markedly inhibited in CCR2-KO and CypD/CCR2-KO mice while residual inflammation was noted in CypD-KO mice. In CypD mice, Pitava-NP reduced recruitment of Ly6Chigh inflammatory monocytes and showed therapeutic effects (Fig. B). In contrast, CsA-NP reduced IR injury in CCR2-KO mice. Simultaneous treatment with CsA-NP and Pitava-NP at the time of reperfusion showed additive reduction in IR injury in wild-type mice (Fig. C). Conclusions: Nanoparticle-mediated simultaneous targeting to mitochondria and inflammatory monocytes can be developed as a novel therapeutic strategy in IR injury.
The S/390 G5 floating point unit supporting hex and binary architectures (2003)

The first high performance floating point unit to support both IBM 360 hexadecimal based floating point architecture and the IEEE 754 Standard binary floating point architecture is described. The S/390 G5 floating point unit supports the new S/390 architecture which includes hexadecimal based short, long, and extended precision formats and IEEE 754 standard single, double, and quad formats. This floating point unit is part of the microprocessor chip on the S/390 G5 mainframe computer introduced in 1998 and generally available at 500 MHz speeds. The S/390 G5 represents the current state of the art in CISC processor design. The paper describes the S/390 architecture enhancements, the internal format of the FPU, and the modifications to the FPU dataflow.
Adaptive hierarchical resource management for satellite channel in hybrid MANET-satellite-internet network (2005)

MANETs are often deployed in an infrastructure-less or hostile region where the satellite provides the only link for the MANETs to communicate with the rest of the world. It faces many challenges to support multiple serviced communications between MANETs and Internet through satellite. In this paper, we propose an efficient resource management scheme called AHRM to dynamically allocate bandwidths among multiple MANET users and multiple priority and non-priority services sharing a multi-access satellite channel. It uses a flexible hierarchical structure to exploit the channel utility and resolve contention from two levels. A bandwidth adaptation algorithm is designed to adjust the allocation dynamically in response to traffic and link status changes. The algorithm turns out to be in line with reinforcement learning and is a customized version of it for the practical satellite network setting. Implementation issues are discussed. Simulation results are presented, showing that the scheme can guarantee fast delivery of critical messages in spite of channel contention, and significantly improve the performance of multiple services.
Service Oriented Pervasive Computing for Emergency Response Systems (2006)

Pervasive computing paradigm is characterized by the presence of a diverse variety of computing and communicating devices. The vision of pervasive computing is to enable effective support for user tasks by utilizing the devices carried by the users and those available within the infrastructure around the users. Emergency response is very critical to crisis situations. The process of emergency response can benefit greatly by the application of pervasive computing principles. In this paper, we describe a prototype designed to enhance the existing emergency response mechanism in attending to automobile accident victims. The prototype has been built using our event oriented middleware called pervasive information communities organization (PICO), and is built on top of a popular P2P framework called JXTA. By utilizing the middleware, we model the useful features of devices available within a car as services. Our proposed framework allows the creation, discovery and maintenance of continuous services such as crash detection. Further more, the framework allows the composition of complex services on occurrence of events by utilizing the available basic services with a goal to provide essential support in case of an accident. We present the design and implementation details of the prototype and snapshots of the working prototype.
Interaction of growth hormone receptor/binding protein gene disruption and caloric restriction for insulin sensitivity and attenuated aging (2015)

<ns4:p>The correlation of physiological sensitivity to insulin (<ns4:italic>vis-à-vis</ns4:italic> glycemic regulation) and longevity is extensively established, creating a justifiable gerontological interest on whether insulin sensitivity is causative, or even predictive, of some or all phenotypes of slowed senescence (including longevity). The <ns4:italic>growth</ns4:italic> <ns4:italic>hormone</ns4:italic> <ns4:italic>receptor/</ns4:italic> <ns4:italic>binding</ns4:italic> <ns4:italic>protein</ns4:italic> gene-disrupted (GHR-KO) mouse is the most extensively investigated insulin-sensitive, attenuated aging model. It was reported that, in a manner divergent from similar mutants, GHR-KO mice fail to respond to caloric restriction (CR) by altering their insulin sensitivity. We hypothesized that maximized insulin responsiveness is what causes GHR-KO mice to exhibit a suppressed survivorship response to dietary (including caloric) restriction; and attempted to refute this hypothesis by assessing the effects of CR on GHR-KO mice for varied slow-aging-associated phenotypes. In contrast to previous reports, we found GHR-KO mice on CR to be <ns4:italic>less</ns4:italic> responsive than their <ns4:italic>ad libitum</ns4:italic> (A.L.) counterparts to the hypoglycemia-inducing effects of insulin. Further, CR had negligible effects on the metabolism or cognition of GHR-KO mice. Therefore, our data suggest that the effects of CR on the insulin sensitivity of GHR-KO mice do not concur with the effects of CR on the aging of GHR-KO mice.</ns4:p>
Using cavity liners with direct posterior composite restorations. (2006)

Cavity liners have traditionally been used in direct and indirect restorations for purposes such as promoting reparative dentin and neutralizing acids. Today, liners are used when resin composites are used as the restorative materials on the posterior teeth, but for a different reason. Clinically, liners are considered to decrease sensitivity and wet the cavity better than restorative composites because of their flowability, adaptation to the dentinal surface, and adhesion. As bonding systems and composite materials continue to improve and become better understood, so do the techniques for placing composites. This has led to the reassessment of the clinical relevance and function of liners. Some clinicians don't use cavity liners, assuming they are a thing of the past; some use composite liners, and others use resin-modified glass ionomer liners. Additionally, there is not a clear agreement over the function of liners, such as when and why they should be used or what type of liner material would provide the best performance for a particular clinical situation. This article attempts to clarify some of the confusion surrounding the use of liners by reviewing the available literature on the subject and attempting to give evidence-based rationale for the use and protocol for the clinician.
RANDOMIZED EXTERNAL-MEMORY ALGORITHMS FOR LINE SEGMENT INTERSECTION AND OTHER GEOMETRIC PROBLEMS (2001)

We show that the well-known random incremental construction of Clarkson and Shor 18 can be adapted to provide efficient external-memory algorithms for some geometric problems. In particular, as the main result, we obtain an optimal randomized algorithm for the problem of computing the trapezoidal decomposition determined by a set of N line segments in the plane with K pairwise intersections, that requires [Formula: see text] expected disk accesses, where M is the size of the available internal memory and B is the size of the block transfer. The approach is sufficiently general to derive algorithms for other geometric problems: 3-d half-space intersections, 2-d and 3-d convex hulls, 2-d abstract Voronoi diagrams and batched planar point location; these algorithms require an optimal expected number of disk accesses and are simpler than the ones previously known. The results extend to an external-memory model with multiple disks.
Synthesis of a Room Temperature Stable 12CaO×7Al2O3 Electride from the Melt and Its Application as an Electron Field Emitter. (2006)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Achieving a Superplastic Forming Capability through Severe Plastic Deformation (2004)

Processing by severe plastic deformation (SPD) leads to very significant grain refinement in metallic alloys. Furthermore, if these ultrafine grains are reasonably stable at elevated temperatures, there is a potential for achieving high tensile ductilities, and superplastic elongations, in alloys that are generally not superplastic. In addition, the production of ultrafine grains leads to the occurrence of superplastic flow at strain rates that are significantly faster than in conventional alloys so that processing by SPD introduces the possibility of using these alloys for the rapid fabrication of complex parts through superplastic forming operations. This paper examines the development of superplasticity in various aluminum alloys processed by equal-channel angular pressing (ECAP).
Supplementary Figure 3 from Identification of Campath-1 (CD52) as Novel Drug Target in Neoplastic Stem Cells in 5q-Patients with MDS and AML (2023)

&lt;p&gt;PDF file - 103K, Supplementary Figure S3: Expression of CD52 on CD34+/CD38− cells in different WHO subtypes of MDS and AML.&lt;/p&gt;
Determination of a factor VIII-interactive region within plasmin responsible for plasmin-catalysed activation and inactivation of factor VIII(a) (2010)

Plasmin, an active form of plasminogen, activates and inactivates factor VIII (FVIII) by limited proteolysis. We have previously identified lysine-binding site-independent plasmin-interactive sites on the FVIII A2 domain responsible for cleavages at Arg336 and Arg372, together with lysine-binding site-dependent plasmin sites on the light chain responsible for cleavage at Lys36. We have now characterised FVIII-interactive regions on plasmin. SDS-PAGE analysis demonstrated that a monoclonal antibody (mAb) against kringle (K)5-catalytic domain (K5-CD) of plasmin significantly blocked plasmin-catalysed cleavages at Arg336 and Arg372. K5-CD fragment and this mAb blocked plasmin-catalysed activation and inactivation of FVIII(a). Anti-K1-2-3 and anti-K4 mAbs blocked plasmin-catalysed cleavages at Lys36, and K1-2-3 and K4 fragments inhibited plasmin-catalysed inactivation of A11-336FVIIIa. The K5-CD preferentially bound to the A2 domain (Kdapp; 52 nM), whilst the K1-2-3 and K4 bound to the light chain (Kdapp; 75 and 106 nM, respectively) in ELISA. Binding was attributed to the A2 484-509 region and A3 1690-1705/1804-1818 region, respectively. 6-aminohexanoic acid, a lysine analogue, significantly inhibited the light chain/K1-2-3 (and K4) binding by approximately 90%, whilst A2/K5-CD binding was moderated by only approximately 35%. Furthermore, an anti-CD antibody blocked plasmin-catalysed cleavage by inhibiting the A2/K5-CD interaction. These data demonstrate that the K5-CD of plasmin (and plasminogen) interacts with the A2 domain independent of lysine-binding site, whilst interactions of K1-2-3 and K4 with the light chain are lysine-binding site-dependent. Interactions between the K5-CD and A2 likely constitute the major regulatory mechanism for activation and inactivation of FVIII(a) mediated by cleavage at Arg372 and Arg336.
Cultivation of Mycoplasmas on Glass (1971)

Eight Mycoplasma species of human origin were successfully cultivated on glass. Complement-fixing (CF) antigens prepared from glass-adherent mycoplasmas were potent, specific, and free from anticomplementary activity. PPLO broth medium supplemented with 1 to 5% PPLO serum fraction (bovine), 2.5% fresh yeast extract, and 1% glucose (glycolytic species) or 1% arginine (arginine-utilizing species) supported moderate to luxuriant growth of mycoplasmas on glass. The potency of CF antigens prepared from glass-adherent mycoplasmas varied with the species of Mycoplasma tested and the duration of incubation. When the potency of CF antigens prepared from glass-adherent mycoplasmas was compared with that material sedimented from the broth phase of the same culture, three patterns of growth were observed: M. hominis and M. orale type 2 grew preferentially in the broth phase; M. salivarium, M. orale types 1 and 3, M. pneumoniae, and M. lipophilum preferentially adhered to the glass; and M. fermentans was biphasic. The growth of mycoplasmas on glass provides a simple means of concentrating and purifying such organisms for immunological and biochemical studies.
Data from Spatially Resolved Single-Cell Assessment of Pancreatic Cancer Expression Subtypes Reveals Co-expressor Phenotypes and Extensive Intratumoral Heterogeneity (2023)

&lt;div&gt;Abstract&lt;p&gt;Pancreatic ductal adenocarcinoma (PDAC) has been classified into classical and basal-like transcriptional subtypes by bulk RNA measurements. However, recent work has uncovered greater complexity to transcriptional subtypes than was initially appreciated using bulk RNA expression profiling. To provide a deeper understanding of PDAC subtypes, we developed a multiplex immunofluorescence (mIF) pipeline that quantifies protein expression of six PDAC subtype markers (CLDN18.2, TFF1, GATA6, KRT17, KRT5, and S100A2) and permits spatially resolved, single-cell interrogation of pancreatic tumors from resection specimens and core needle biopsies. Both primary and metastatic tumors displayed striking intratumoral subtype heterogeneity that was associated with patient outcomes, existed at the scale of individual glands, and was significantly reduced in patient-derived organoid cultures. Tumor cells co-expressing classical and basal markers were present in &gt; 90% of tumors, existed on a basal-classical polarization continuum, and were enriched in tumors containing a greater admixture of basal and classical cell populations. Cell–cell neighbor analyses within tumor glands further suggested that co-expressor cells may represent an intermediate state between expression subtype poles. The extensive intratumoral heterogeneity identified through this clinically applicable mIF pipeline may inform prognosis and treatment selection for patients with PDAC.&lt;/p&gt;Significance:&lt;p&gt;A high-throughput pipeline using multiplex immunofluorescence in pancreatic cancer reveals striking expression subtype intratumoral heterogeneity with implications for therapy selection and identifies co-expressor cells that may serve as intermediates during subtype switching.&lt;/p&gt;&lt;/div&gt;
Mixed Organic/Inorganic Abrasive Particles during Oxide CMP (2008)

Chemical mechanical planarization (CMP) of silicon dioxide films was performed using mixed abrasive slurries (MASs) achieved by mixing in situ polymer and inorganic particles. The results were discussed in terms of material removal rate (MRR) and defectivity. We compared sílica colloids and primary size ceria particles. Our experiments shed some light on the importance of the bond between polymer core and inorganic particles in the shell and on the differences between silica-based and ceria-based MASs. Although for the silica-based MAS the MRR passes through a maximum ( for 2:3 ratio polymer: silica), for the ceria-based MAS it increases with the ceria solid content. The polymer:silica ratios used in the experiments spanned in the range from 1:15 to 10:1.
Cyclic Feature Concealing CP Selection for Physical Layer Security (2014)

Cyclic prefix (CP) utilizing techniques such as orthogonal frequency division multiplexing (OFDM) and single carrier frequency domain equalization (SC-FDE) offer significant advantages in equalizing the effect of time dispersive channels at the expense of a reasonable spectral redundancy. However, cyclic features introduced by CP to the signal can also be exploited for blind parameter estimation and synchronization which compromise the security and privacy of the signal against eavesdropping attacks. In this paper, we propose a novel CP selection technique that conceals the cyclic features of the signal while maintaining the advantages in equalization without reducing spectral efficiency. After presenting the proposed technique, its performance is discussed for OFDM and SCFDE in terms of bit-error rate and complexity as well as cyclic feature suppression.
[Direct in vivo gene introduction into rat kidney]. (1992)

We established a simple and efficient method in vivo gene transfer using Sendai virus (HVJ) and liposomes. Plasmid DNA and high mobility group 1 (MHG1) protein were co-encapsulated in liposomes and co-introduced into target cells by HVJ-mediated membrane fusion. pAct-SVT DNA, as a reporter gene, was introduced into the kidney of intact rats through a cannula in the renal artery, and SV 40 large T antigen was detected by enzyme immunohistochemically in glomerular cells (mesangial cells and/or capillary cells) 4 days after it's introduction. Thereafter expression of SV 40 large T antigen gene decreased and was not detected on day 10. This gene delivery system could be useful in research and development of gene therapy of renal disease.
Synthesis of [11C] platelet‐activating factor (PAF) analogs for in vivo imaging of PAF receptors (1993)

Abstract 1‐O‐Hexadecyl‐2‐O‐N, N‐dimethylcarbamoyl‐sn‐glycero‐3‐phosphocholine[choline methyl‐ 11 C] ([ 11 C]dimethylcarbamoyl‐PAF) and 1‐O‐Hexadecyl‐2‐O‐acetyl‐sn‐glycero‐3‐phosphocholine[choline methyl‐ 11 C] ([ 11 C]C 16 ‐PAF) were synthesized as follows; Each of non‐labeled dimethylcarbamoyl‐PAF and C 16 ‐PAF was treated with sodium benzene thiolate to derive their desmethyl‐precursors containing a dimethylphosphoethanolamine at sn‐3. 11 C‐Labeled dimethylcarbamoyl‐PAF and C 16 ‐PAF were synthesized by methylation of the respective desmethyl‐precursors using [ 11 C]CH 3 I. The radiochemical yield of methylation in [ 11 C]dimethylcarbamoyl‐PAF and [ 11 C]C 16 ‐PAF was about 15 and 10% (decay corrected), respectively. The lower yield of [ 11 C]C 16 ‐PAF compared with that of [ 11 C]dimethylcarbamoyl‐PAF was attributed to hydrolysis of the 2‐acetyl group of [ 11 C]C 16 ‐PAF during methylation. To study the stability to enzymatic hydrolysis, [ 11 C]dimethylcarbamoyl‐PAF or [ 11 C]C 16 ‐PAF was incubated with mouse plasma at 37 °C.
Position Sequencing By Hybridization (1995)

Succinoylamino hydroxyethylamino sulfonamide compounds are effective as retroviral protease inhibitors, and in particular as inhibitors of HIV protease.