gebhart/patrepeval huggingface dataset:
Poly(ethylene oxide) Grafted with Short Polyethylenimine Gives DNA Polyplexes with Superior Colloidal Stability, Low Cytotoxicity, and Potent In Vitro Gene Transfection under Serum Conditions (2012)

Poly(ethylene oxide) grafted with 1.8 kDa branched polyethylenimine (PEO-g-PEI) copolymers with varying compositions, that is, PEO(13k)-g-10PEI, PEO(24k)-g-10PEI, and PEO(13k)-g-22PEI, were prepared and investigated for in vitro nonviral gene transfer. Gel electrophoresis assays showed that PEO(13k)-g-10PEI, PEO(24k)-g-10PEI, and PEO(13k)-g-22PEI could completely inhibit DNA migration at an N/P ratio of 4/1, 4/1, and 3/1, respectively. Dynamic light scattering (DLS) and zeta potential measurements revealed that all three graft copolymers were able to effectively condense DNA into small-sized (80-245 nm) particles with moderate positive surface charges (+7.2 ∼ +24.1 mV) at N/P ratios ranging from 5/1 to 40/1. The polyplex sizes and zeta-potentials intimately depended on PEO molecular weights and PEI graft densities. Notably, unlike 25 kDa PEI control, PEO-g-PEI polyplexes were stable against aggregation under physiological salt as well as 20% serum conditions due to the shielding effect of PEO. MTT assays in 293T cells demonstrated that PEO-g-PEI polyplexes had decreased cytotoxicity with increasing PEO molecular weights and decreasing PEI graft densities, wherein low cytotoxicities (cell viability >80%) were observed for polyplexes of PEO(13k)-g-22PEI, PEO(13k)-g-10PEI, and PEO(24k)-g-10PEI up to an N/P ratio of 20/1, 30/1, and 40/1, respectively. Interestingly, in vitro transfection results showed that PEO(13k)-g-10PEI polyplexes have the best transfection activity. For example, PEO(13k)-g-10PEI polyplexes formed at an N/P ratio of 20/1, which were essentially nontoxic (100% cell viability), displayed over 3- and 4-fold higher transfection efficiencies in 293T cells than 25 kDa PEI standard under serum-free and 10% serum conditions, respectively. Confocal laser scanning microscopy (CLSM) studies using Cy5-labeled DNA confirmed that these PEO-g-PEI copolymers could efficiently deliver DNA into the perinuclei region as well as into nuclei of 293T cells at an N/P ratio of 20/1 following 4 h transfection under 10% serum conditions. PEO-g-PEI polyplexes with superior colloidal stability, low cytotoxicity, and efficient transfection under serum conditions are highly promising for safe and efficient in vitro as well as in vivo gene transfection applications.
Localization of Protein Complex Bound Ligands by Surface-Induced Dissociation High-Resolution Mass Spectrometry (2018)

Surface-induced dissociation (SID) is a powerful means of deciphering protein complex quaternary structures due to its capability of yielding dissociation products that reflect the native structures of protein complexes in solution. Here we explore the suitability of SID to locate the ligand binding sites in protein complexes. We studied C-reactive protein (CRP) pentamer, which contains a ligand binding site within each subunit, and cholera toxin B (CTB) pentamer, which contains a ligand binding site between each adjacent subunit. SID dissects ligand-bound CRP into subcomplexes with each subunit carrying predominantly one ligand. In contrast, SID of ligand-bound CTB results in the generation of subcomplexes with a ligand distribution reflective of two subunits contributing to each ligand binding site. SID thus has potential application in localizing sites of small ligand binding for multisubunit protein-ligand complexes.
Urinary Phytate in Calcium Oxalate Stone Formers and Healthy People: Dietary Effects on Phytate Excretion (2000)

The phytate urinary levels in a group of active calcium oxalate stone formers were studied and compared with those found in healthy people. Urinary phytate was significantly lower for stone formers. If deficit of the capacity to inhibit crystallization of calcium salts is considered an important factor related to calcium stone formation, the excretion of low phytate amounts could be an important risk factor in the development of this type of renal calculi. The influence of dietary phytate on urinary excretion was also studied. Clearly maintenance of a phytate-free diet significantly decreased the urinary excretion of phytate (about 50% after 36 h). This demonstrated the importance of dietary phytate in maintaining adequate urinary levels to permit effective crystallization inhibition of calcium salts and consequently preventing renal stone development.
Hyperspectral image compression suitable for spectral analysis application (2010)

In the compression technique of hyperspectral image (HSI), PSNR of the reconstructed image is usually used for evaluating the performance of the coding results. For the spectral analysis applications of HSI, it is also important to consider the error in the result of spectral analysis. In the vegetation analysis, for example, the distortion of the vegetation index should be considered in addition to the distortion in the spectral data. This paper presents a HSI compression considering the error of both vegetation index and spectral data. The proposed method separates a hyperspectral data into spectral data for vegetation index and residual data. Both of the data are encoded by using a seamless coding individually. By holding the spectral channels required for vegetation index in the head of the code-stream, a precise vegetation analysis can be done in a low bit rate. Additionally, by decoding the residual data, the spectral data can be reconstructed in low distortion.
Temporal coupled-mode theory for the Fano resonance in optical resonators (2003)

We present a theory of the Fano resonance for optical resonators, based on a temporal coupled-mode formalism. This theory is applicable to the general scheme of a single optical resonance coupled with multiple input and output ports. We show that the coupling constants in such a theory are strongly constrained by energy-conservation and time-reversal symmetry considerations. In particular, for a two-port symmetric structure, Fano-resonant line shape can be derived by using only these symmetry considerations. We validate the analysis by comparing the theoretical predictions with three-dimensional finite-difference time-domain simulations of guided resonance in photonic crystal slabs. Such a theory may prove to be useful for response-function synthesis in filter and sensor applications.
FASTA: A Generalized Implementation of Forward-Backward Splitting (2015)

This is a user manual for the software package FASTA.
Nucleation, Stabilization and Growth of Microbubbles in Water. (1978)

This final report of a study of nucleation, stabilization and growth of microbubbles in water reviews the advances made and external communications originating from the author's research.
Irijimasides A–E, Macrolide Glycosides from an Okeania sp. Marine Cyanobacterium (2020)

Irijimasides A-E (<b>1</b>-<b>5</b>), a series of new 14-membered macrolide glycosides, were isolated from a marine cyanobacterium collected in Okinawa. The gross structures of <b>1</b>-<b>5</b> were established by spectroscopic analysis, including 2D NMR, while absolute stereostructures were determined based on NOESY spectra, chemical derivatization, and ECD data. All five macrolides suppressed receptor activator of nuclear factor-κB ligand (RANKL)-induced tartrate-resistant acid phosphatase (TRAP) activity in mouse RAW264 macrophage cells, indicating that these compounds inhibit osteoclast formation.
Dietary soya protein intake and exercise training have an additive effect on skeletal muscle fatty acid oxidation enzyme activities and mRNA levels in rats (2006)

Exercise training and regular physical activity increase oxidation of fat. Enhanced oxidation of fat is important for preventing lifestyle diseases such as hypertension and obesity. The aim of the present study in rats was to determine whether intake of dietary soya protein and exercise training have an additive effect on the activity and mRNA expression of enzymes involved in skeletal muscle fatty acid oxidation. Male Sprague-Dawley rats (n 32) were assigned randomly into four groups (eight rats per group) and then divided further into sedentary or exercise-trained groups fed either casein or soya protein diets. Rats in the exercise groups were trained for 2 weeks by swimming for 120 min/d, 6 d/week. Exercise training decreased hepatic triacylglycerol levels and retroperitoneal adipose tissue weight and increased skeletal muscle carnitine palmitoyltransferase 1 (CPT1) activity and mRNA expression of CPT1, beta-hydroxyacyl-CoA dehydrogenase (HAD), acyl-CoA oxidase, PPARgamma coactivator 1alpha (PGC1alpha) and PPARalpha. Soya protein significantly decreased hepatic triacylglycerol levels and epididymal adipose tissue weight and increased skeletal muscle CPT1 activity and CPT1, HAD, acyl-CoA oxidase, medium-chain acyl-CoA dehydrogenase, PGC1alpha and PPARalpha mRNA levels. Furthermore, skeletal muscle HAD activity was the highest in exercise-trained rats fed soya protein. We conclude that exercise training and soya protein intake have an important additive role on induction of PPAR pathways, leading to increased activity and mRNA expression of enzymes involved in fatty acid oxidation in skeletal muscle and reduced accumulation of body fat.
Electro-optical characterization of poled-polymer films in transmission (1996)

An improved version of the commonly employed C. C. Teng and H. I. Man (Appl. Phys. Lett. 56, 1734 [1990]) measurement technique is illustrated by the characterization of films of a new high glass transition temperature polyurea. Measurements taken in the transmission mode are shown to be free from non-negligible errors introduced by interference effects present in standard reflection geometry measurements.
Synthesis and Characterization of Poly(ethylene oxide)-block-poly(methylidene malonate 2.1.2) Block Copolymers Bearing a Mannose Group at the PEO Chain End (2004)

A poly(ethylene oxide)-block-poly(methylidene malonate 2.1.2) block copolymer (PEO-b-PMM 2.1.2) bearing a mannose moiety at the poly(ethylene oxide) chain end was synthesized by sequential anionic polymerization of ethylene oxide (EO) and methylidene malonate 2.1.2 (MM 2.1.2), followed by a coupling reaction between its poly(ethylene oxide) amino- or aldehyde-end group and a sugar derivative. Different coupling procedures, either in organic media or in aqueous micellar solutions, were examined in order to optimize the poly(ethylene oxide) end-glycosylation yield. The micellar size of the functionalized block copolymers was determined by dynamic light scattering.
Supercritical Fluid Chemical Deposition as an Alternative Process to CVD for the Surface Modification of Materials (2011)

Abstract Taking the desirable attributes of CVD and aqueous plating techniques while minimizing the disadvantages of each, supercritical fluid chemical deposition (SFCD) demonstrates itself to be a promising process alternative to enable the fabrication of nanostructured devices. This paper gives information about the SFCD process for metal deposition as an alternative to CVD through various examples of inorganic deposition (films or nanostructures). In particular, the use of SFCD will be demonstrated for the surface modification of carbon nanotubes (CNTs) and their decoration with palladium nanoparticles.
Changes in Regulation of a Transcription Factor Lead to Autogamy in Cultivated Tomatoes (2007)

We report the cloning of Style2.1, the major quantitative trait locus responsible for a key floral attribute (style length) associated with the evolution of self-pollination in cultivated tomatoes. The gene encodes a putative transcription factor that regulates cell elongation in developing styles. The transition from cross-pollination to self-pollination was accompanied, not by a change in the STYLE2.1 protein, but rather by a mutation in the Style2.1 promoter that results in a down-regulation of Style2.1 expression during flower development.
Calculation of Blade Track Height Using an Optical Tracker (2018)

Rotor Track and Balance (RTB) is a necessary maintenance action to improve aircraft ride quality and decrease fatigue for both the aircrew and helicopter equipment. While there have been a number of papers discussing the solution strategy for optimizing both blade track split and vibration, little work has been reported on how to calculate blade track by using an optical sensor. This paper will discuss the formulation of the blade track height estimate based on optical tracker measurements from flight-testing. Furthermore, the article will include a sensitivity analysis to define the blade track height error. Finally, contending solutions strategies are tested by comparing the results between two pulse and three pulse optical trackers.
Assessment and improvement of image homogeneity in black-blood T2-weighted turbo spin-echo CMR (2012)

DOAJ is a unique and extensive index of diverse open access journals from around the world, driven by a growing community, committed to ensuring quality content is freely available online for everyone.
Beating the quantum limit in SIS mixers (1989)

The 2LO (double local oscillator) mixer is analyzed in the low-LO-power limit for a perfect SIS (superconductor-insulator-superconductor) diode. It is shown that a 2LO SIS mixer can beat the noise performance of a one-LO mixer by a factor of two. It is suggested that even lower noise is possible with an SIS when operated in some other way.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Nanostructured Materials Integrated in Microfabricated Optical Devices (2002)

This project combined nanocomposite materials with microfabricated optical device structures for the development of microsensor arrays. For the nanocomposite materials we have designed, developed, and characterized self-assembling, organic/inorganic hybrid optical sensor materials that offer highly selective, sensitive, and reversible sensing capability with unique hierarchical nanoarchitecture. Lipid bilayers and micellar polydiacetylene provided selective optical response towards metal ions (Pb(II), Hg(II)), a lectin protein (Concanavalin A), temperature, and organic solvent vapor. These materials formed as composites in silica sol-gels to impart physical protection of the self-assembled structures, provide a means for thin film surface coatings, and allow facile transport of analytes. The microoptical devices were designed and prepared with two- and four-level diffraction gratings coupled with conformal gold coatings on fused silica. The structure created a number of light reflections that illuminated multiple spots along the silica surface. These points of illumination would act as the excitation light for the fluorescence response of the sensor materials. Finally, we demonstrate an integrated device using the two-level diffraction grating coupled with the polydiacetylene/silica material.
Inkjet printing of fluorinated materials and their application to patterning organic semiconductors (2013)

With the rise of functional printing there is a need to understand the inkjet printability of a greater range of materials. Fluorinated materials and solvents have some unusual properties but this study finds them to be reliably inkjet printable. The fluorinated ink used has Reynolds and Weber numbers of 9.2 ± 1.8 and 20.3 ± 3.0, respectively. Printed line widths on silicon can be varied from 45 μm to 90 μm by adjusting printing parameters. A key advantage of printing from fluorinated solvents is that they can be printed on other organic layers without damaging or dissolving the underlayer. Test patterns are demonstrated on PMMA, P3HT, pentacene and F8BT. This naturally suggests their application to patterning organic semiconductors by selectively protecting some areas. The utility of this technique is demonstrated in the fabrication of an array of P3HT transistors with 2 μm channel lengths in which the active area is patterned using an inkjet printed single-drop fluoropolymer etch mask.
Task-based assessment and optimization of spectral domain optical coherence tomography for tear film imaging (2012)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text J. Huang, K. Lee, E. Clarkson, M. Kupinski, and J. P. Rolland, "Task-based assessment and optimization of spectral domain optical coherence tomography for tear film imaging," in Frontiers in Optics 2012/Laser Science XXVIII, OSA Technical Digest (online) (Optica Publishing Group, 2012), paper FTu3A.39. Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
An International Terminology for Endometriosis, 2021 (2021)

The current paper outlines a list of 49 terms and definitions in the field of endometriosis. The application of the defined terms aims to facilitate harmonization in endometriosis research and clinical practice. Future research may require further refinement of the presented definitions.
Valgus deformity of the ankle in myelodysplastic patients. Correction by stapling of the medial part of the distal tibial physis. (1983)

Thirteen patients with myelodysplasia and associated valgus deformities of the ankle had stapling of the medial part of the distal tibial physis. The pattern of neural involvement in these patients was responsible for characteristic muscle imbalances across the tibiotalar and subtalar joints which led to the valgus deformities. Stapling was performed in twenty-five ankles, fifteen of which had previously been treated unsuccessfully with various soft-tissue and bone procedures. Twelve of the patients were followed until closure of the physis. The average preoperative valgus deformity at the tibiotalar joint was 10 degrees and the average amount of correction was 16 degrees. There were no major complications in our series. All patients retained the ability to walk postoperatively, and there was no increase in the need for bracing. Stapling of the medial part of the distal tibial epiphysis has proved to be a safe and simple procedure for correction of valgus deformities of the ankle in myelodysplastic patients.
The Impact of Multiple Comorbid Mental Health Disorders on Health-related Quality of Life Following ACDF (2020)

Level III.
Quantitation of suspected allergens in fragrances (Part I): evaluation of comprehensive two‐dimensional gas chromatography for quality control (2004)

Abstract An evaluation of comprehensive two‐dimensional (2D) gas chromatography (GC×GC) was performed to assess its suitability for the analysis of volatile fragrance components, recognized by the European Commission's Scientiﬁc Committee on Cosmetics and other Non‐food Products (SCCNFP) as possible skin sensitizers. The 24 volatile components listed by the SCCNFP were baseline‐resolved or better within one 30 min analysis. High‐quality calibration data for standard mixtures were obtained, with R 2 &gt; 0.998 over the concentration range 2–1000 mg/l. However, the analysis of small spiked amounts of target compounds in truly complex fragrances was problematic, due to uncertainty in component assignment. The beneﬁts and limitations of GC×GC are reported, and a discussion of the proposed directions for the solution of this analysis is provided. Copyright © 2004 John Wiley &amp; Sons, Ltd.
Lectin-like Oxidized Low-density Lipoprotein Receptor-1 (LOX-1) and Cardiac Fibroblast Growth (2012)

Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) regulates growth of a variety of cells and is important in inflammation, oxidative stress, and tissue remodeling. Recent studies show that LOX-1 deletion limits cardiac remodeling after sustained hypertension. We posited that LOX-1 may affect cardiac fibroblast growth and collagen secretion. To examine this postulate, we studied growth pattern of cardiac fibroblasts from hearts of wild-type and LOX-1 knockout (KO) mice. LOX-1 KO fibroblasts exhibited dramatically reduced growth when compared with wild-type mice fibroblasts and became much larger than wild-type mice fibroblasts in serial cultures, suggesting arrest of cell division. Western blotting and immunofluorescence showed that cell division control protein 42, a key regulator for cell division, was markedly downregulated in LOX-1 KO fibroblasts. The cytoskeletal organization in these fibroblasts was significantly altered in strand orientation, and some fibroblasts were completely devoid of F-actin. Furthermore, NADPH oxidase expression and generation of reactive oxygen species, as well as cell proliferation signals serine/threonine-specific protein kinase and murine double minute 2, were significantly reduced in LOX-1 KO fibroblasts. To confirm the essential role of LOX-1 in fibroblast growth, LOX-1 KO fibroblasts were transfected with h-LOX-1 cDNA. After transfection, the altered pattern of cytoskeletal organization, as well as expression of cell division control protein 42, serine/threonine-specific protein kinase, and murine double minute 2, was normalized. In congruent with these in vitro data, we found that the cardiac fibroblast number and expression of fibronectin and procoallagen-1/collagen were significantly lower in hypertensive LOX-1 KO mice hearts than in hypertensive wild-type mice hearts subjected to sustained hypertension (angiotensin II infusion). These findings implicate LOX-1 in cytoskeletal organization and growth of cardiac fibroblasts.
On the security of 1024-bit RSA and 160-bit elliptic curve cryptography (2009)

Meeting the requirements of NIST’s new cryptographic standard ‘Suite B Cryptography’ means phasing out usage of 1024-bit RSA and 160-bit Elliptic Curve Cryptography (ECC) by the year 2010. This write-up comments on the vulnerability of these systems to an open community attack effort and aims to assess the risk of their continued usage beyond 2010. We conclude that for 1024-bit RSA the risk is small at least until the year 2014, and that 160-bit ECC may safely be used for much longer – with the current state of the art in cryptanalysis we would be surprised if a public effort can make a dent in 160-bit ECC by the year 2020. Our assessment is based on the latest practical data of large scale integer factorization and elliptic curve discrete logarithm computation efforts.
Computational Fluid Dynamics Simulations and Experiments of Meltblown Fibrous Media: New Die Designs to Enhance Fiber Attenuation and Filtration Quality (2016)

The meltblowing process employs high-speed hot air jets to attenuate polymer streams injected from a die head. In this study, we examine design strategies to control the air flow field below the polymer injection point to achieve higher fiber attenuation and meltblown webs with smaller fiber diameters. Computational fluid dynamics (CFD) simulations for new die configurations show that vertical or inclined air constrictors around the primary air jets keep the centerline air velocity and temperature at their maximum values for 10–15 mm longer below the die face than the reference die. Polymer streams are kept near their melting temperatures at higher air velocities for a longer period, resulting in higher fiber attenuation. The underlying mechanisms leading to such behavior are discussed. Experimental results show reduction in fiber diameter and pore size, validating the simulation. Improved filtration properties are also obtained from the nonwovens webs.