gebhart/patrepeval huggingface dataset:
Endoplasmic reticulum–mitochondria junction is required for iron homeostasis (2017)

The endoplasmic reticulum (ER)-mitochondria encounter structure (ERMES) is a protein complex that physically tethers the two organelles to each other and creates the physical basis for communication between them. ERMES functions in lipid exchange between the ER and mitochondria, protein import into mitochondria, and maintenance of mitochondrial morphology and genome. Here, we report that ERMES is also required for iron homeostasis. Loss of ERMES components activates an Aft1-dependent iron deficiency response even in iron-replete conditions, leading to accumulation of excess iron inside the cell. This function is independent of known ERMES roles in calcium regulation, phospholipid biosynthesis, or effects on mitochondrial morphology. A mutation in the vacuolar protein sorting 13 (<i>VPS13</i>) gene that rescues the glycolytic phenotype of ERMES mutants suppresses the iron deficiency response and iron accumulation. Our findings reveal that proper communication between the ER and mitochondria is required for appropriate maintenance of cellular iron levels.
Dynamic recruitment of single RNAs to processing bodies depends on RNA functionality (2018)

SUMMARY Cellular RNAs often colocalize with cytoplasmic, membrane-less ribonucleoprotein (RNP) granules enriched for RNA processing enzymes, termed processing bodies (PBs). Here, we track the dynamic localization of individual miRNAs, mRNAs and long non-coding RNAs (lncRNAs) to PBs using intracellular single-molecule fluorescence microscopy. We find that unused miRNAs stably bind to PBs, whereas functional miRNAs, repressed mRNAs and lncRNAs both transiently and stably localize within either the core or periphery of PBs, albeit to different extents. Consequently, translation potential and positioning of cis -regulatory elements significantly impact PB-localization dynamics of mRNAs. Using computational modeling and supporting experimental approaches we show that phase separation into large PBs attenuates mRNA silencing, suggesting that physiological mRNA turnover predominantly occurs outside of PBs. Instead, our data support a role for PBs in sequestering unused miRNAs to regulate their surveillance and provides a framework for investigating the dynamic assembly of RNP granules by phase separation at single-molecule resolution.
Light scattering, straylight, and optical quality in hydrophobic acrylic intraocular lenses with subsurface nanoglistenings (2016)

Dr. Das is an employee of Alcon Laboratories, Inc. The Complete Angle Instrument scatterometer was developed by Dr. Stover at the Scatterworks, Inc. Neither of the other authors has a financial or proprietary interest in any method or material mentioned.
Fluid and Tissue Biomarkers of Lewy Body Dementia: Report of an LBDA Symposium (2022)

The Lewy Body Dementia Association (LBDA) held a virtual event, the LBDA Biofluid/Tissue Biomarker Symposium, on January 25, 2021, to present advances in biomarkers for Lewy body dementia (LBD), which includes dementia with Lewy bodies (DLBs) and Parkinson's disease dementia (PDD). The meeting featured eight internationally known scientists from Europe and the United States and attracted over 200 scientists and physicians from academic centers, the National Institutes of Health, and the pharmaceutical industry. Methods for confirming and quantifying the presence of Lewy body and Alzheimer's pathology and novel biomarkers were discussed.
Amygdala and orbitofrontal engagement in breach and resolution of expectancy: A case study. (2015)

Humans constantly predict their environment to facilitate mutual interaction. Predictions are connected with emotions as nonfatal penalties and rewards (for incorrect and correct expectancies, respectively) that result in negative and positive emotions. Music is an ideal stimulus to explore the underlying neurophysiological mechanisms of prediction related emotions. Using the high spatial and temporal resolution of stereotactic depth electrodes, we identified activation patterns and examined their distribution in the bilateral Amygdalae and the orbitofrontal cortex (OFC). We used music excerpts with either (a) a deceptive cadence (i.e., an unexpected chord/breach) or (b) a tonic chord inserted instead of a deceptive cadence (regular chord/no breach). These events were followed by a chord progression leading to and ending on the tonic after a breach (c) or (d) on a tonic after no breach. We computed the differences of the analytic amplitudes in the theta band at these time-points (i.e., events a–d) by using t tests. We found a significant difference between the unexpected chord (a) and the expected chord (b) in the analytic amplitude of the theta band in the left amygdala. Further we found a difference between the 2 resolutions (c and d) in the analytic amplitude of the theta band within the OFC. In conclusion, our case study supports the notion that the amygdala and the OFC are important for emotional responses to musical expectancy breaches as well as of their resolution. (PsycINFO Database Record (c) 2016 APA, all rights reserved)
A 1.2-V, 12-bit, 200 MSample/s Current-Steering D/A Converter in 90-nm CMOS (2007)

This paper describes a 1.2-V, 12-bit, 200-MSample/s current-steering CMOS digital-to-analog (D/A) converter for wireless-communication terminals. To our knowledge, the supply voltage of this converter is the lowest for high-speed applications. To overcome increasing device mismatch in low-voltage operation, we propose an H-shaped, 3-dimensional structure for reducing influence of voltage drops (IR drops) along power supplies. This technique relaxes mismatch requirements and allows use of small devices with small parasitics. By using this technique, a low-voltage, high-speed D/A converter was realized. The converter was implemented in a 90-nm CMOS technology. The modulator achieves the intrinsic accuracy of 12 bits and a spurious-free dynamic range (SFDR) above 55 dB over a 100-MHz bandwidth.
Improvement of ground to satellite fso link performance using transmit diversity in weak atmospheric turbulence (2010)

The performance of ground to satellite Free Space Optical (FSO) communication link is degraded due to the presence of atmospheric turbulence that causes fluctuations in both intensity and phase of the received optical signal. In this paper, bit error rate (BER) performance of FSO link using transmit diversity is investigated for coherent (sub-carrier BPSK and sub-carrier QPSK) and non-coherent (OOK and Q-PPM) modulation schemes in weak atmospheric turbulence channel. It is seen that multiple transmitting antennae help to overcome the turbulence induced fading effect. It is observed that sub-carrier BPSK modulation outperforms the other modulation schemes in terms of minimum SNR requirement for a given BER. Among non-coherent modulation schemes, Q-PPM performs better than OOK. However, as the level of Q in Q-PPM increases, its performance degrades and it becomes worse than OOK for certain values of atmospheric turbulence. Also, the effect of correlation among different transmitting antennae beams is analysed on the link performance.
NMR Analysis of Structure and Dynamics of the Cytosolic Tails of Integrin αIIbβ3 in Aqueous Solution (2001)

The structural and dynamic properties of the cytosolic tails of the adhesion receptor integrin alphaIIbbeta3, fused to a coiled-coil construct via (Gly)(3) linkers, were studied in aqueous solution by nuclear magnetic resonance (NMR) spectroscopy. Both tails were largely flexible and unstructured, although, in the beta3 tail, residues Arg(724)-Ala(735) have a propensity to form a helical structure and residues Asn(744)-Tyr(747) (NPLY) have a propensity to adopt reverse-turn conformations. The mutation beta3(Y747A) disrupted this reverse-turn tendency and markedly reduced the affinity of the head domain of the cytoskeletal protein, talin for the beta3 tail. Omission of the (Gly)(3) linker connecting the coiled-coiled helices and the integrin tails lead to helix propagation into the beta3 tail extending up to eight residues. A variety of different tail constructs were made and studied to reveal tail-tail interactions, but surprisingly no significant interactions between both tails could be detected within the context of our constructs. These results provide structural insight into a highly conserved beta tail motif (NPXY/F) required for integrin signaling and highlight a second transiently structured region (residues Arg(724)-Ala(735)), which might also be of functional significance.
Opening an Electrical Band Gap of Bilayer Graphene with Molecular Doping (2011)

The opening of an electrical band gap in graphene is crucial for its application for logic circuits. Recent studies have shown that an energy gap in Bernal-stacked bilayer graphene can be generated by applying an electric displacement field. Molecular doping has also been proposed to open the electrical gap of bilayer graphene by breaking either in-plane symmetry or inversion symmetry; however, no direct observation of an electrical gap has been reported. Here we discover that the organic molecule triazine is able to form a uniform thin coating on the top surface of a bilayer graphene, which efficiently blocks the accessible doping sites and prevents ambient p-doping on the top layer. The charge distribution asymmetry between the top and bottom layers can then be enhanced simply by increasing the p-doping from oxygen/moisture to the bottom layer. The on/off current ratio for a bottom-gated bilayer transistor operated in ambient condition is improved by at least 1 order of magnitude. The estimated electrical band gap is up to ∼111 meV at room temperature. The observed electrical band gap dependence on the hole-carrier density increase agrees well with the recent density-functional theory calculations. This research provides a simple method to obtain a graphene bilayer transistor with a moderate on/off current ratio, which can be stably operated in air without the need to use an additional top gate.
Analysis of immunization time, amplitude, and adverse events of seven different vaccines against SARS-CoV-2 across four different countries (2022)

Abstract Background Scarce information exists in relation to the comparison of seroconversion and adverse events following immunization (AEFI) with different SARS-CoV-2 vaccines. Our aim was to correlate the magnitude of the antibody response to vaccination with previous clinical conditions and AEFI. Methods A multicentric comparative study where SARS-CoV-2 spike 1-2 IgG antibodies IgG titers were measured at baseline, 21-28 days after the first and second dose (when applicable) of the following vaccines: BNT162b2 mRNA, mRNA-1273, Gam-COVID-Vac, Coronavac, ChAdOx1-S, Ad5-nCoV and Ad26.COV2. Mixed model and Poisson generalized linear models were performed. Results We recruited 1867 subjects [52 (SD 16.8) years old, 52% men]. All vaccines enhanced anti-S1 and anti-S2 IgG antibodies over time (p&lt;0.01). The highest increase after the first and second dose was observed in mRNA-1273 (p&lt;0.001). There was an effect of previous SARS-CoV-2 infection; and an interaction of age with SARS-CoV-2, Gam-COVID-Vac and ChAdOx1-S (p&lt;0.01). There was a negative correlation of Severe or Systemic AEFI (AEs) of naïve SARS-CoV-2 subjects with age and sex (p&lt;0.001); a positive interaction between the delta of antibodies with Gam-COVID-Vac (p=0.002). Coronavac, Gam-COVID-Vac and ChAdOx1-S had less AEs compared to BNT162b (p&lt;0.01). mRNA-1273 had a higher number of AEFIs. The delta of the antibodies showed an association with AEFIs in previously infected individuals (p&lt;0.001). Conclusions The magnitude of seroconversion is predicted by age, vaccine type and SARS-CoV-2 exposure. AEs are correlated with age, sex, and vaccine type. The delta of the antibody response is positively correlated with AEs in patients previously exposed to SARS-CoV-2. Registration number NCT05228912
SRv6 Network Programming Overhead Analysis (2020)

SRv6 network programming provides the framework for the best
compression of an IPv6 header within an SR domain. This document
provides the analysis to illustrate this fact.
Necrotizing Choroiditis-Retinitis as Presenting Symptom of Disseminated Aspergillosis after Lung Transplantation (1997)

Background. Endogenous endophthalmitis due to Aspergillus is rare affecting the severely immunosuppressed population, in particular recipients of heart and lung transplants. Ocular involvement of aspergillosis has always been observed late in the course of the disease. Subject. A young woman noted blurred vision in one eye three weeks after lung transplantation. At this stage, no systemic manifestations of fungal infection were detected and the ocular findings were attributed to viral infection. Results. Twenty-four hours after the original ocular complaint, an aggressive endophthalmitis developed in the left eye. The possibility of fungal endophthalmitis was raised. Within 48 hours of her first ocular complaint the patient died. Cultures from a vitreous tap and from autopsy ocular specimens were positive for Aspergillus fumigatus. Conclusions. Aspergillus endophthalmitis may occur in patients undergoing lung transplantation despite antifungal therapy. Increased awareness of this unusual entity may be life and vision saving in these patients.
A low-power structured light sensor for outdoor scene reconstruction and dominant material identification (2012)

We introduce a compact structured light device that utilizes a commercially available MEMS mirror-enabled hand-held laser projector. Without complex re-engineering, we show how to exploit the projector's high-speed MEMS mirror motion and laser light-sources to suppress ambient illumination, enabling low-cost and low-power reconstruction of outdoor scenes in sunlight. We discuss how the line-striping acts as a kind of “light-probe”, creating distinctive patterns of light scattered by different types of materials. We investigate visual features that can be computed from these patterns and can reliably identify the dominant material characteristic of a scene, i.e. where most of the objects consist of either diffuse (wood), translucent (wax), reflective (metal) or transparent (glass) materials.
Development of lifting type load-relief device for simulation of low gravity environment (2021)

近年，月面や宇宙空間での有人探査が多くの国で行われている．しかし，低重力環境下で宇宙飛行士が安全に活動するための方法については未知な点が多い．1960年代のアポロ計画当時1)から現在に至るまで，宇宙飛行士の低重力環境下での船外活動シミュレーションが行われてきた．
Nanopharmaceuticals and Nanomedicines Currently on the Market: Challenges and Opportunities (2018)

There has been a revolution in nanotechnology and nanomedicine. Since 1980, there has been a remarkable increase in approved nano-based pharmaceutical products. These novel nano-based systems can either be therapeutic agents themselves, or else act as vehicles to carry different active pharmaceutical agents into specific parts of the body. Currently marketed nanostructures include nanocrystals, liposomes and lipid nanoparticles, PEGylated polymeric nanodrugs, other polymers, protein-based nanoparticles and metal-based nanoparticles. A range of issues must be addressed in the development of these nanostructures. Ethics, market size, possibility of market failure, costs and commercial development, are some topics which are on the table to be discussed. After passing all the ethical and biological assessments, and satisfying the investors as to future profitability, only a handful of these nanoformulations, successfully obtained marketing approval. We survey the range of nanomedicines that have received regulatory approval and are marketed. We discuss ethics, costs, commercial development and possible market failure. We estimate the global nanomedicine market size and future growth. Our goal is to summarize the different approved nanoformulations on the market, and briefly cover the challenges and future outlook.
Avoiding unnecessary ventricular pacing is associated with reduced incidence of heart failure hospitalizations and persistent atrial fibrillation in pacemaker patients (2023)

Cardiac pacing with AV delay management via AVH is associated with reduced 1-year incidence of HFH and persistent AF, most likely due to a reduction in VPP compared to standard pacing.
Three-Dimensional Force Measurements During Rapid Palatal Expansion in Sus scrofa (2016)

Rapid palatal expansion is an orthodontic procedure widely used to correct the maxillary arch. However, its outcome is significantly influenced by factors that show a high degree of variability amongst patients. The traditional treatment methodology is based on an intuitive and heuristic treatment approach because the forces applied in the three dimensions are indeterminate. To enable optimal and individualized treatment, it is essential to measure the three-dimensional (3D) forces and displacements created by the expander. This paper proposes a method for performing these 3D measurements using a single embedded strain sensor, combining experimental measurements of strain in the palatal expander with 3D finite element analysis (FEA). The method is demonstrated using the maxillary jaw from a freshly euthanized pig (<i>Sus scrofa</i>) and a hyrax-design rapid palatal expander (RPE) appliance with integrated strain gage. The strain gage measurements are recorded using a computer interface, following which the expansion forces and extent of expansion are estimated by FEA. A total activation of 2.0 mm results in peak total force of about 100 N-almost entirely along the direction of expansion. The results also indicate that more than 85% of the input activation is immediately transferred to the palate and/or teeth. These studies demonstrate a method for assessing and individualizing expansion magnitudes and forces during orthopedic expansion of the maxilla. This provides the basis for further development of smart orthodontic appliances that provide real-time readouts of forces and movements, which will allow personalized, optimal treatment.
Ti-based interface engineering for heteroepitaxial growth of SrTiO3 on GaAs (2014)

29-31 Janvier 2014
Supplementary Figure 4 from Radiotherapy Increases the Permissiveness of Established Mammary Tumors to Rejection by Immunomodulatory Antibodies (2023)

&lt;p&gt;PDF file - 55K, Side-by-side immunohistochemical analysis of the 4T1.2 (A) and AT-3 (B) tumor microenviroment&lt;/p&gt;
Development of interdigitated electrode based enzyme sensor for triglyceride monitoring using engineered oxygen insensitive glycerol 3-phosphate oxidase (2023)

Triglycerides (TG) are an important biomarker of various diseases including hyper-lipidemia. Current emergent requirements for TG sensing include the development of electrochemical sensors for easy, cost-effective, disposable and single use triglyceride sensors. We propose a TG sensor using an engineered glycerol 3-phosphate oxidase (GlpO) and electrochemical detection. In this study, we aimed to develop an electrochemical TG sensor based on engineering the oxidative half reaction of GlpO to be oxygen insensitive, with the employment of an interdigitated electrode (IDE). To construct an oxygen insensitive mutant GlpO, we constructed a double mutant, Met104Phe/Asp169Glu, which revealed an engineered GlpO exhibiting repressed oxidase activity, while its dye-mediated dehydrogenase activity enhanced. The electrochemical enzyme sensor was constructed for the measurement of TG based on engineered GlpO using disposable screen-printed carbon electrode (SPCE) or disposable IDE using Ru complex as the electron mediator. By using the disposable IDE sensor, a highly sensitive electrochemical sensor for Glp was achieved, with the sensitivity of 0.4 µA/mM. The improved sensitivity was 2.4 times higher than that of SPCE (0.17 µA/mM), with LOD of 0.022 mM. Furthermore, the dry sensor strip, where the double mutant GlpO and Ru complex were dropcasted and dried on the IDE, was prepared and the Glp concentration was successfully measured in the delipidized serum solution. Therefore, by the employment of the established pretreatment method for TG to release Glp, a highly sensitive, accurate, easy and rapid electrochemical measurement method for TG will be developed using constructed M104F/D169E mutant GlpO with the combination of IDE.
Dewatering of Ionic Liquids in a Falling Film Evaporator (2011)

Abstract Die Entwässerung ionischer Flüssigkeiten (IL) wurde in einem Einrohr‐Metallfallfilmverdampfer experimentell untersucht. Abhängig von den Prozessbedingungen konnten in einer Batchfahrweise Restwassergehalte von ca. 0,2 Gew.‐% (2000 ppm) erreicht werden. Unter der Annahme einer Entwässerung bis ins Gleichgewicht konnten Dampfdruckkurven von IL/Wasser‐Lösungen und daraus Ausdampfwärmen abgeschätzt werden. Die während der Entwässerung online gemessene Viskosität erwies sich als geeignetes Maß zur Verfolgung des Entwässerungsprozesses.
Cardiac characteristics and long-term outcome in Andersen-Tawil syndrome patients related to KCNJ2 mutation (2013)

Despite a severe clinical presentation with a very high rate of ventricular arrhythmias, the arrhythmic prognosis of the ATS patients is relatively good under treatment.
A Study of Patients With a Radiologic Diagnosis of Mesenteric Panniculitis and the Correlation With Malignancy (2014)

Introduction: Mesenteric panniculitis (MP), a “misty” or “hazy” mesentery and/or an unexplained mesenteric mass is an uncommon finding on computerized tomographic (CT) scanning. This finding in patients with prior cancer has been described, but its significance is poorly understood. We reviewed its association with malignancy, disease natural history; additional and follow-up imaging studies in a large group of patients with mesenteric abnormalities and either known history of cancer, or those with newly diagnosed cancers. Methods: CT scans showing “mesenteric panniculitis” or “misty mesentery” in patients with known or newly diagnosed cancer at North Shore University Health System were identified from January 2005-April 2010. Medical records were reviewed. Results of positron emission tomography (PET) were noted. In total, 359 patients (from 147,794 CT scans) had MP-like abnormalities. Of these patients, 81 had a known history of cancer and 19 had a new diagnosis of cancer. Results: Lymphomas were the most common cancers associated with MP (36%), with follicular lymphoma being the most common subtype (17/36), (Table 1). A variety of solid tumors were found, the most common being prostate (7) and renal cell cancer (6). CT follow-up was obtained in 71 patients. The CT was unchanged in 62, worsened in 7, and improved in 2 patients. PET scans were performed in 44 patients. Positive uptake in mesenteric lymph nodes was seen in 13 patients (29.6%). Positive uptake in the mesenteric mass was only seen in 2 patients (4.5%). Follow-up PET scanning showed no uptake in the mesenteric mass in both of these patients.Table 1Conclusion: Mesenteric changes suggestive of mesenteric panniculitis are seen in association with a variety of cancers. These changes generally remain stable on follow-up CT. Mesenteric uptake of fludeoxyglucose (FDG) during PET scanning does not occur in these lesions.