gebhart/patrepeval huggingface dataset:
The endoribonucleolytic N-terminal half of Escherichia coli RNase E is evolutionarily conserved in Synechocystis sp. and other bacteria but not the C-terminal half, which is sufficient for degradosome assembly (RNA degradosomeymRNA degradation) (1998)

Escherichia coli RNase E, an essential single- stranded specific endoribonuclease, is required for both ri- bosomal RNA processing and the rapid degradation of mRNA. The availability of the complete sequences of a number of bacterial genomes prompted us to assess the evolutionarily conservation of bacterial RNase E. We show here that the sequence of the N-terminal endoribonucleolytic domain of RNase E is evolutionarily conserved in Synechocystis sp. and other bacteria. Furthermore, we demonstrate that the Syn- echocystis sp. homologue binds RNase E substrates and cleaves them at the same position as the E. coli enzyme. Taken together these results suggest that RNase E-mediated mech- anisms of RNA decay are not confined to E. coli and its close relatives. We also show that the C-terminal half of E. coli RNase E is both sufficient and necessary for its physical interaction with the 3*-5* exoribonuclease polynucleotide phosphorylase, the RhlB helicase, and the glycolytic enzyme enolase, which are components of a ''degradosome'' complex. Interestingly, however, the sequence of the C-terminal half of E. coli RNase E is not highly conserved evolutionarily, sug- gesting diversity of RNase E interactions with other RNA decay components in different organisms. This notion is supported by our finding that the Synechocystis sp. RNase E homologue does not function as a platform for assembly of E. coli degradosome components.
PLAD (Plasma Doping) on 22nm Technology Node and Beyond - Evolutionary and/or Revolutionary (2012)

PLAD (plasma doping) is promising for both evolutionary and revolutionary doping options because of its unique advantages which can overcome or minimize many of the issues of the beam-line (BL) based implants. In this talk, we present developments of PLAD on both planar and 3D device structures. Comparing with the conventional BL implants, PLAD shows not only a significant production enhancement, but also a significant device performance improvement and 3D structure doping capability, including an 80% contact resistance reduction, more than 25% drive current increase, and conformal doping on non-planar device structures. In PLAD developments and applications, the conventional metrologies are not suitable for PLAD process because of their limits and PLAD's unique properties. Novel diagnostic metrologies for PLAD process have been developed and are also presented in this talk.
53.1: Invited Paper : Recent Trend of LED Backlight with Local Dimming and its Application for Multi‐Primary‐Color Displays (2011)

Abstract We will consider the characteristics of LED backlights discussing merits and demerits of those characteristics which we will summarize. We have also evaluated combination with multi‐primary color panels. By combining with multi‐primary color panels, it will be possible to create new effects in Local Dimming technology.
BMP-mediated inhibition of FGF signaling promotes cardiomyocyte differentiation of anterior heart field progenitors (2010)

The anterior heart field (AHF) encompasses a niche in which mesoderm-derived cardiac progenitors maintain their multipotent and undifferentiated nature in response to signals from surrounding tissues. Here, we investigate the signaling mechanism that promotes the shift from proliferating cardiac progenitors to differentiating cardiomyocytes in chick embryos. Genomic and systems biology approaches, as well as perturbations of signaling molecules, in vitro and in vivo, reveal tight crosstalk between the bone morphogenetic protein (BMP) and fibroblast growth factor (FGF) signaling pathways within the AHF niche: BMP4 promotes myofibrillar gene expression and cardiomyocyte contraction by blocking FGF signaling. Furthermore, inhibition of the FGF-ERK pathway is both sufficient and necessary for these processes, suggesting that FGF signaling blocks premature differentiation of cardiac progenitors in the AHF. We further revealed that BMP4 induced a set of neural crest-related genes, including MSX1. Overexpression of Msx1 was sufficient to repress FGF gene expression and cell proliferation, thereby promoting cardiomyocyte differentiation. Finally, we show that BMP-induced cardiomyocyte differentiation is diminished following cranial neural crest ablation, underscoring the key roles of these cells in the regulation of AHF cell differentiation. Hence, BMP and FGF signaling pathways act via inter- and intra-regulatory loops in multiple tissues, to coordinate the balance between proliferation and differentiation of cardiac progenitors.
Bi-Phasic Effect of Equol on Adipocyte Differentiation of MC3T3-L1 Cells (2013)

We investigated the effects of equol on adipogenesis by measuring lipid accumulation and analyzing the change in adipocyte-related gene expression in MC3T3-L1 cells. Treatment with 10 µM equol tended to increase adipocyte-related gene expression, whereas 100 µM equol reduced lipid accumulation and suppressed the expression of these genes and proteins. Our results suggest that equol regulated adipogenesis in a bi-phasic fashion.
Photoluminescence and Scintillation Properties of Ce-doped SrHfO3 (2019)

Ce-doped SrHfO 3 crystals were synthesized by the floating zone (FZ) method, and their photoluminescence (PL) and scintillation properties were investigated. X-ray diffraction (XRD) measurement indicates that the samples are mainly composed of SrHfO 3 , but a small peak due to HfO 2 is observed. In both PL and scintillation analyses, the Ce-doped samples show the emission peaking around 410 nm, whose decay time is short due to the 5d-4f transitions of Ce 3+ . In the undoped sample, the emission appears around 480 nm. In the afterglow curves after 2 ms X-ray irradiation, no significant concentration dependence is observed.
Polyimides containing nonaromatic nitrogen linkages (1992)

Abstract Two diamines, 2,5‐bis (4‐aminophenyl)‐2,5‐diazahexane and 1,4‐bis (4‐aminophenyl)‐1,4‐diazacyclohexane were chosen as components for polyimidizations because they have melting points that differ by nearly 200°C (66–67 and 229–230°C, respectively) and are relatives of p ‐nitro‐ N , N ‐dimethylaniline. The melting points of the model compounds (phthalic anhydride) do not differ by as much as those of the free amines [303–304 and 386°C (DSC), respectively]. Six polyimides were prepared by a two‐step polycondensation of the diamines with pyromellitic dianhydride, benzophenonetetracarboxylic dianhydride, and 5,5'‐[2,2,2‐trifluoro‐1‐(trifluoromethyl) ethylidene] bis‐1,3‐isobenzofurandione. DSC thermograms failed to indicate any distinct transitions up to 450°C, however, the polyimide prepared from 2,5‐bis (4‐aminophenyl)‐2,5‐diazahexane and pyromellitic dianhydride shows a slight break in its DSC curve at 233°C.
Altered expression of ABO(H) carbohydrate antigens is seen in pleomorphic adenomas (1992)

Cell surface carbohydrate antigens show changes in relation to differentiation, maturation and malignant transformation. The expression of type 2 chain ABH carbohydrate structures of the ABO histo-blood group system was investigated in 28 pleomorphic adenomas (PA) and normal parotid glands in order to study possible changes in the glycosylation pattern. The distribution of carbohydrate structures was investigated by immunohistological stainings of formalin-fixed paraffin-embedded material using monoclonal antibodies (MAbs) with well-defined specificity. A strong interindividual variation was found in the normal tissue as well as in the tumors. In normal tissue, acinus and duct cells all expressed elongated carbohydrate structures. The yoepithelial cells did not stain with any of the MAbs investigated. In the PAs, staining was seen in the ductular structures and myoepithelial cells. In contrast to normal tissue, the tumors expressed the short precursor molecule sialylated N-acetyllactosamine. Furthermore, the PAs showed loss of H and A antigens, and a reduced expression of Le(y) compared to normal tissue. The ductular structures as well as the modified myoepithelial cells expressed binary N-acetyllactosamine, which in the normal tissue could only be found in the striated and excretory ducts. Thus our study has shown that aberrant glycosylation is not only a feature of malignant neoplasms but also occurs in pleomorphic adenomas.
Aperture Synthesis In Space (1989)

Optical aperture synthesis (OAS) may be used to obtain images of much higher resolution than &quot;seeing&quot;-limited observations presently made from the ground. The principles of OAS are essentially those used in radio astronomy and may be applied to space-based or to ground-based observations. The greater spatial resolution obtained would facilitate the imaging of stellar envelopes around Be stars, the study of the internal dynamics of active galaxies, etc. The application of aperture synthesis techniques in space, at visible and at ultra-violet wavelengths, should permit imaging of much fainter sources than would be possible from a terrestrial telescope array. Infra-red observations are best made from the ground. A summary of the conclusion reached by the Space Interferometry Study Team set up by ESA will be presented. A short description of the important parameters relevant to a space mission (attitude control, orbit, structural dynamics, etc) and a comparison to terrestrial atmospheric conditions will be given. Possible instrument configurations will be described and it will be shown that a large field of view may be achieved, so that the instrument may be calibrated on bright stars whilst observing faint sources. Mission concepts for a &quot;monostructure&quot; ~30 metres in size will be examined and a possible strategy for Space Interferometry in the next 20 years considered.
Methodology for the Physical Cell Identity Assignment in LTE Networks (2013)

There are very few pharmacists who were there when modern day consultant pharmacy was formed, but Samuel W. Kidder, PharmD, MPH, who died October 14, 2012, at the age of 76, was one of them. As a government regulator, he helped create the role of the consultant pharmacist by developing regulations for drug regimen review and standards for detecting medication errors. Because of Kidder's ongoing involvement in nursing facility survey and certification, he also was a key resource for ASCP. He was a strong supporter of consultant pharmacists and championed reimbursement for their services. Considered a visionary in the field, he was known as an advocate both for pharmacists and for patients.
Long-term follow-up of ibrutinib monotherapy in treatment-naive patients with Waldenstrom macroglobulinemia (2021)

Herein, we present the final report of a single-center, prospective phase II study evaluating ibrutinib 420 mg once daily in 30 treatment-naive patients with Waldenstrom macroglobulinemia (WM). The present study is registered with ClinicalTrials.Gov (NCT02604511). With a median follow-up of 50 months, the overall, major, and VGPR response rates were 100%, 87%, and 30%. The VGPR rate was numerically but not significantly lower in patients with than without CXCR4 mutations (14% vs. 44%; p = 0.09). The median time to a minor response was 0.9 months, and to a major response was 1.9 months, though were longer in those with mutated CXCR4 at 1.7 months (p = 0.07) and 7.3 months (p = 0.01). Six patients had disease progression. The median progression-free survival (PFS) was not reached, and the 4-year PFS rate was 76%. There was also a non-significant lower 4-year PFS rate in patients with than without CXCR4 mutations (59% vs. 92%; p = 0.06). The most common treatment-related adverse events were fatigue, upper respiratory infection, and hematoma. Atrial fibrillation occurred in 20% of patients. Ibrutinib monotherapy induced durable responses in treatment-naive patients with WM. CXCR4 mutations impacted VGPR attainment, time to major response, and 4-year PFS rate.
Crystal structure of bromotri(phenyl)phosphinesulphidegold(I), (C6H5)3PSAuBr (2001)

Article Crystal structure of bromotri(phenyl)phosphinesulphidegold(I), (C6H5)3PSAuBr was published on April 1, 2001 in the journal Zeitschrift für Kristallographie - New Crystal Structures (volume 216, issue 1-4).
A pilot study of coupled plasma filtration with adsorption in septic shock* (2002)

In patients with septic shock, coupled plasmafiltration-adsorption combined with hemodialysis was associated with improved hemodynamics compared with continuous venovenous hemodiafiltration. This result might be related to its ability to restore leukocyte responsiveness to lipopolysaccharide. These findings suggest a potential role for blood purification in the treatment of septic shock.
Allosteric “beta-blocker” isolated from a DNA-encoded small molecule library (2017)

The β<sub>2</sub>-adrenergic receptor (β<sub>2</sub>AR) has been a model system for understanding regulatory mechanisms of G-protein-coupled receptor (GPCR) actions and plays a significant role in cardiovascular and pulmonary diseases. Because all known β-adrenergic receptor drugs target the orthosteric binding site of the receptor, we set out to isolate allosteric ligands for this receptor by panning DNA-encoded small-molecule libraries comprising 190 million distinct compounds against purified human β<sub>2</sub>AR. Here, we report the discovery of a small-molecule negative allosteric modulator (antagonist), compound 15 [([4-((2<i>S</i>)-3-(((<i>S</i>)-3-(3-bromophenyl)-1-(methylamino)-1-oxopropan-2-yl)amino)-2-(2-cyclohexyl-2-phenylacetamido)-3-oxopropyl)benzamide], exhibiting a unique chemotype and low micromolar affinity for the β<sub>2</sub>AR. Binding of 15 to the receptor cooperatively enhances orthosteric inverse agonist binding while negatively modulating binding of orthosteric agonists. Studies with a specific antibody that binds to an intracellular region of the β<sub>2</sub>AR suggest that 15 binds in proximity to the G-protein binding site on the cytosolic surface of the β<sub>2</sub>AR. In cell-signaling studies, 15 inhibits cAMP production through the β<sub>2</sub>AR, but not that mediated by other Gs-coupled receptors. Compound 15 also similarly inhibits β-arrestin recruitment to the activated β<sub>2</sub>AR. This study presents an allosteric small-molecule ligand for the β<sub>2</sub>AR and introduces a broadly applicable method for screening DNA-encoded small-molecule libraries against purified GPCR targets. Importantly, such an approach could facilitate the discovery of GPCR drugs with tailored allosteric effects.
Distribution and abundance of neutral endopeptidase (EC 3.4.24.11) in the alimentary tract of the rat (1993)

The distribution of neutral endopeptidase (NEP; EC 3.4.24.11) was examined in the alimentary tract of the rat. Immunoreactive NEP and NEP mRNA were localized to epithelial cells of the small intestine and to muscle cells in the stomach, small intestine, and colon by immunohistochemistry and in situ hybridization histochemistry. NEP antisera recognized a protein on Western blots of membranes from gastric, jejunal, and colonic mucosa and gastric muscle with an electrophoretic mobility identical to that of recombinant human NEP (approximately 95 kDa). An antisense cRNA probe to NEP hybridized to RNA of approximately 3.5 kb and approximately 6.5 kb, corresponding to the primary transcripts of rat NEP, on Northern blots of total RNA from the jejunal mucosa. NEP message was detected in mRNA from jejunal and colonic mucosa and gastric, jejunal, and colonic muscle using a ribonuclease protection assay. NEP enzymatic activity, assessed by DL-thiorphan-inhibitable degradation of glutaryl-Ala-Ala-Phe-4-methoxy-2-naphthylamine, was highest in homogenates of jejunal mucosa (868 +/- 98 pmol.h-1 x micrograms protein-1) and was between 49- and 413-fold lower in other gastrointestinal tissues. The cellular origin of NEP in the gastric and colonic mucosa could not be determined.
Mechanisms of Aqueous Humor Formation (2008)

Glaucoma is a leading cause of irreversible blindness in the world (1). Primary openangle glaucoma (POAG) is the most common form of glaucoma and its onset, as well as progression, is often insidious, leading to significant visual loss before being clinically diagnosed. POAG is frequently associated with elevated intraocular pressure (IOP) of the eye. It is incurable at present, although its progression can be retarded by lowering the IOP by medication and/or surgery. If pharmacological agents fail to attain a targeted hypotensive response within the framework of the clinical characteristics and course, surgery will be indicated. The IOP is physiologically maintained within a relatively narrow range bracketing a mean of approximately 15 mmHg. The level of IOP reflects a dynamic balance between secretion (inflow) and drainage (outflow) of aqueous humor. The aqueous humor is a transparent fluid that is formed by the ciliary processes (epithelium) of the eye. After its production, aqueous humor flows from the posterior chamber to the anterior chamber of the eye via the pupil (Fig. 1). In the anterior chamber, the temperature difference between the warmer iris and the cooler cornea results in a convectional fluid circulation.
Gar nicht spartanisch: Spartan (1996)

Abstract Spartan 4.1.1 ist ein Molecular Modelling‐Programm, das “state of the art” Rechenverfahren mit einer exzellenten Visualisierung der Ergebnisse bei gleichzeitig akzeptablem Preis verbindet.
Screening for non-classic congenital adrenal hyperplasia revisited: proposal for a new serum 17-hydroxyprogesterone threshold for which a cosyntropin stimulation test is indicated (2022)

Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
Computationally Efficient Transcranial Ultrasonic Focusing: Taking Advantage of the High Correlation Length of the Human Skull (2020)

The phase correction necessary for transcranial ultrasound therapy requires numerical simulation to noninvasively assess the phase shift induced by the skull bone. Ideally, the numerical simulations need to be fast enough for clinical implementation in a brain therapy protocol and to provide accurate estimation of the phase shift to optimize the refocusing through the skull. In this article, we experimentally performed transcranial ultrasound focusing at 900 kHz on N = 5 human skulls. To reduce the computation time, we propose here to perform the numerical simulation at 450 kHz and use the corresponding phase shifts experimentally at 900 kHz. We demonstrate that a 450-kHz simulation restores 94.2% of the pressure when compared with a simulation performed at 900 kHz and 85.0% of the gold standard pressure obtained by an invasive time reversal procedure based on the signal recorded by a hydrophone placed at the target. From a 900- to 450-kHz simulation, the grid size is divided by 8, and the computation time is divided by 10.
An Arabidopsis SBP‐domain fragment with a disrupted C‐terminal zinc‐binding site retains its tertiary structure (2006)

SQUAMOSA promoter-binding proteins (SBPs) form a major family of plant-specific transcription factors, mainly related to flower development. SBPs share a highly conserved DNA-binding domain of approximately 80 amino acids (SBP domain), which contains two non-interleaved zinc-binding sites formed by eight conserved Cys or His residues. In the present study, an Arabidopsis SPL12 SBP-domain fragment that lacks a Cys residue involved in the C-terminal zinc-binding pocket was found to retain a folded structure, even though only a single Zn2+ ion binds to the fragment. Solution structure of this fragment determined by NMR is very similar to the previously determined structures of the full SBP domains of Arabidopsis SPL4 and SPL7. Considering the previous observations that chelating all the Zn2+ ions of SBPs resulted in the complete unfolding of the structure and that a mutation of the Cys residue equivalent to that described above impaired the DNA-binding activity, we propose that the Zn2+ ion at the N-terminal site is necessary to maintain the overall tertiary structure, while the Zn2+ ion at the C-terminal site is necessary for the DNA binding, mainly by guiding the basic C-terminal loop to correctly fit into the DNA groove.
Anaerobic spirochaetes and animals (2015)

Anaerobic spirochaetes colonise the large intestine of many avian and mammalian host species. The most well known pathogenic species is the strongly haemolytic Brachyspira hyodysenteriae that was first isolated from pigs with swine dysentery (SD) in the early 1970s. Classical SD is a severe mucohaemorrhagic colitis that occurs in growing pigs and is endemic in most pig-rearing areas of the world. The spirochaete acts in concert with other components of the colonic microbiota to disrupt the integrity of the colonic epithelium and induce inflammation. In recent years two new strongly haemolytic species, the proposed ‘Brachyspira suanatina' and ‘Brachyspira hampsonii', both with reservoirs in migratory water birds, have been described as new and emerging agents of SD in the northern hemisphere. Weakly haemolytic species also have been described, some of which have pathogenic potential. In particular Brachyspira pilosicoli causes a mild colitis and diarrhoea in many species, including human beings, whilst Brachyspira intermedia is a common pathogen in adult poultry. Infection with B. intermedia and/or B. pilosicoli can cause wet litter, faecal staining of eggshells and delays in reaching peak egg production. Options for control of these widespread and economically significant anaerobic infections are limited.
Crystallization and morphology of binary blends of linear and branched polyethylene (2007)

The paper reviews crystallization kinetics and morphology studies of binary blends of a low molar mass linear polyethylene sharp fraction (M w=2500) and higher molar mass samples of various linear and branched polyethylenes. Cocrystallization and segregation phenomena occurring in these polymers are reported as a function of molar mass and degree of chain branching of the high molar mass component, blend composition, and crystallization temperature. Branched polyethylene with 1.5 mol% of ethyl groups cocrystallizes with the low molar mass linear PE fraction, whereas segregation of the low molar mass polymer is the dominant feature in the blends with polymers having a lower degree of chain branching (≤0.5 mol%). The applicability of differential scanning calorimetry, polarized light microscopy, and transmission electron microscopy to reveal cocrystallization and segregation phenomena in polyethylene is discussed.