gebhart/patrepeval huggingface dataset:
Muc2-dependent microbial colonization of the jejunal mucus layer is diet sensitive and confers local resistance to enteric pathogen infection (2023)

Intestinal mucus barriers normally prevent microbial infections but are sensitive to diet-dependent changes in the luminal environment. Here we demonstrate that mice fed a Western-style diet (WSD) suffer regiospecific failure of the mucus barrier in the small intestinal jejunum caused by diet-induced mucus aggregation. Mucus barrier disruption due to either WSD exposure or chromosomal Muc2 deletion results in collapse of the commensal jejunal microbiota, which in turn sensitizes mice to atypical jejunal colonization by the enteric pathogen Citrobacter rodentium. We illustrate the jejunal mucus layer as a microbial habitat, and link the regiospecific mucus dependency of the microbiota to distinctive properties of the jejunal niche. Together, our data demonstrate a symbiotic mucus-microbiota relationship that normally prevents jejunal pathogen colonization, but is highly sensitive to disruption by exposure to a WSD.
Efficient Synthesis of Pyropheophorbide-a and Its Derivatives (2004)

A new and simplified method of preparing hexyloxy pyropheophorbide-a (HPPH), a promising agent used in photodynamic therapy, is described. This method is carried out in two processing steps, replacing an older method requiring five steps. This is accomplished by means of a Dieckmann condensation and subsequent thermal decarboxylation, both occurring in the same high-boiling solvent, thus reversing the long-standing trend in which naturally occurring chlorin derivatives with exocyclic rings are often subjected to conditions that open this ring to obtain chlorin derivatives. In the new process, a raw material without an exocyclic ring is used to construct a product containing the exocyclic ring. The new method does not require cryogenic processing or chromatography, removing the most significant obstacles to large-scale preparation of HPPH and its homologues.
Comparing perilymph proteomes across species (2017)

NA. Laryngoscope, 128:E47-E52, 2018.
A pendant peptide endows a sunscreen with water-resistance (2018)

Ultraviolet light causes skin cancer. Salicylic acid and other molecular filters absorb damaging radiation but are washed away readily. Conjugation to a collagen mimetic peptide is shown to retain salicylic acid on collagen-containing skin surrogates after repeated washing. This strategy, which is highly modular, could enhance the water-resistance of sunscreens.
Innovative Rapid Prototyping Process Makes Large Sized, Smooth Surfaced Complex Shapes in a Wide Variety of Materials (1998)

(1998). Innovative Rapid Prototyping Process Makes Large Sized, Smooth Surfaced Complex Shapes in a Wide Variety of Materials. Materials Technology: Vol. 13, No. 2, pp. 53-56.
Silent Progression Of Calcific Aortic Stenosis Detected By Artificial Intelligence Electrocardiogram: A Case Report (2022)

The Foundation in Cardiology Practice program will offer a comprehensive review of the fundamentals and most important changes in cardiovascular medicine. Program topics will provide details to ensure patients receive the highest, most up-to-date quality of care and achieve the best possible outcomes when returning to your practice. Electronic posters offer a great opportunity to connect with a look into the future and learn from colleagues to keep the best interest of the patient in mind when providing care.
Periodic bandwidth allocation based on virtual queue occupancy (2003)

Carrying IP traffic over connection-oriented networks requires the use of bandwidth allocation schemes at gateways or network interfaces. A new virtual queue occupancy, which is more accurate than the classical queue status parameter, is being proposed for periodic bandwidth allocation. Based on this virtual queue occupancy, an enhanced approach for lossless services, referred to as LAVQ, is simulated and evaluated in an IP/SONET environment. Simulations show that LAVQ outperforms its counterpart LAQ in terms of bandwidth utilization, without compromising the performance of queue occupancy.
A Positively Charged Loop on the Surface of Kallistatin Functions to Enhance Tissue Kallikrein Inhibition by Acting as a Secondary Binding Site for Kallikrein (2000)

Kallistatin is a serine proteinase inhibitor (serpin) that specifically inhibits tissue kallikrein. The inhibitory activity of kallistatin is abolished upon heparin binding. The loop between the H helix and C2 sheet of kallistatin containing clusters of basic amino acid residues has been identified as a heparin-binding site. In this study, we investigated the role of the basic residues in this region in tissue kallikrein inhibition. Kallistatin mutants containing double Ala substitutions for these basic residues displayed a 70-80% reduction of association rate constants, indicating the importance of these basic residues in tissue kallikrein inhibition. A synthetic peptide derived from the sequence between the H helix and C2 sheet of kallistatin was shown to suppress the kallistatin-kallikrein interaction through competition for tissue kallikrein binding. To further evaluate the function of this loop, we used alpha1-antitrypsin, a non-heparin-binding serpin and slow tissue kallikrein inhibitor as a scaffold to engineer kallikrein inhibitors. An alpha1-antitrypsin chimera harboring the P3-P2' residues and a sequence homologous to the positively charged region between the H helix and C2 sheet of kallistatin acquired heparin-suppressed inhibitory activity toward tissue kallikrein and exhibited an inhibitory activity 20-fold higher than that of the other chimera, which contained only kallistatin's P3-P2' sequence, and 2300-fold higher than that of wild-type alpha1-antitrypsin. The alpha1-antitrypsin chimera with inhibitory characteristics similar to those of kallistatin demonstrates that the loop between the H helix and C2 sheet of kallistatin is crucial in tissue kallikrein inhibition, and this functional loop can be used as a module to enhance the inhibitory activity of a serpin toward tissue kallikrein. In conclusion, our results indicate that a positively charged loop between the H helix and C2 sheet of a serpin can accelerate the association of a serpin with tissue kallikrein by acting as a secondary binding site.
A fast and accurate automatic matching network designed for ultra low power medical applications (2009)

We present a method to automatically match a system to the load variation accurately with a very short matching time for Ultra Low Power medical applications. A demonstrator was fabricated and an experimental set-up in the Medical Implant Communication System (MICS) frequency band of 402–405MHz was realized including a pacemaker antenna prototype immersed on a homogeneous lossy liquid. The matching network consists of a low pass π structure with tunable components made of varactors. Methodologies for simplifying the matching network design are introduced. The measurements show that a S11 up to −30dB is obtained with a matching time of 900µs.
Precursor-Directed Biosynthesis of Epothilone in Escherichia coli (2004)

Engineered biosynthetic pathways provide a powerful method for generating complex molecules. Precursor-directed biosynthesis, which combines chemical synthesis and enzymatic transformations, allows non-native starting materials to be incorporated into biosynthetic pathways. Using this approach, we achieved the production of the anticancer agent epothilone C in Escherichia coli. An E. coli strain was engineered to express the last three modules of the epothilone biosynthetic pathway (epoD-M6, epoE, and epoF) and the substrate required to complement the biosynthetic enzymes was obtained by chemical synthesis. Under high-density cell culture conditions, the E. coli strain processed exogenously fed synthetic substrate into epothilone C at levels comparable to the native host (1 mg/L) and at higher levels than other heterologous hosts. Importantly, this precursor-directed approach will allow chemical modifications to be introduced into the polyketide backbone and may ultimately provide access to epothilone analogues with improved pharmacological properties in quantities sufficient for clinical development.
LATEST DEVELOPMENTS IN SCALE REDUCTION FOR FIRE TESTING (2017)

International audience
Data from Nature of Tumor Control by Permanently and Transiently Modified GD2 Chimeric Antigen Receptor T Cells in Xenograft Models of Neuroblastoma (2023)

&lt;div&gt;Abstract&lt;p&gt;Chimeric antigen receptor (CAR) therapy has begun to demonstrate success as a novel treatment modality for hematologic malignancies. The success observed thus far has been with T cells permanently engineered to express chimeric receptors. T cells engineered using RNA electroporation represent an alternative with the potential for similar efficacy and greater safety when initially targeting novel antigens. Neuroblastoma is a common pediatric solid tumor with the potential to be targeted using immunotherapy. We performed xenograft studies in NSG mice in which we assessed the efficacy of both permanently modified and transiently modified CAR T cells directed against the neuroblastoma antigen GD2 in both local and disseminated disease models. Disease response was monitored by tumor volume measurement and histologic examination, as well as &lt;i&gt;in vivo&lt;/i&gt; bioluminescence. RNA-modified GD2 CAR T cells mediated rapid tumor destruction when delivered locally. A single infusion of lentivirally modified GD2 CAR T cells resulted in long-term control of disseminated disease. Multiple infusions of RNA GD2 CAR T cells slowed the progression of disseminated disease and improved survival, but did not result in long-term disease control. Histologic examination revealed that the transiently modified cells were unable to significantly penetrate the tumor environment when delivered systemically, despite multiple infusions of CAR T cells. Thus, we demonstrate that RNA-modified GD2 CAR T cells can mediate effective antitumor responses &lt;i&gt;in vivo&lt;/i&gt;, and permanently modified cells are able to control disseminated neuroblastoma in xenograft mice. Lack of long-term disease control by RNA-engineered cells resulted from an inability to penetrate the tumor microenvironment. &lt;i&gt;Cancer Immunol Res; 2(11); 1059–70. ©2014 AACR&lt;/i&gt;.&lt;/p&gt;&lt;/div&gt;
Tissue-Engineered Microvasculature to Reperfuse Isolated Renal Glomeruli (2015)

Kidney transplantation is often the most effective therapy for end-stage renal disease, but there are not enough donor organs to meet the rising demand. Tissue engineering of kidneys is a potential solution to this organ shortage. Achieving microvascular perfusion has been a major barrier to engineering tissues beyond thin muscularized sheets such as the bladder wall. Our laboratory has previously reported that human umbilical vein endothelial cells (ECs) transduced with the antiapoptotic protein Bcl-2 will spontaneously organize into perfused microvessels within type I collagen gels when implanted in immunodeficient mice. To test if this system can be used to perfuse more complex structures, we combined Bcl-2-transduced ECs (Bcl-2-ECs) with renal glomeruli, the specialized vascular filtration units of the kidney. Microdissected green fluorescent protein-expressing rat glomeruli suspended in type I collagen gels were implanted within immunodeficient mice with or without the inclusion of Bcl-2-ECs. Survival of rat glomeruli was enhanced by coimplantation with Bcl-2-ECs. Intravital rhodamine dextran injections demonstrated that surviving glomeruli were perfused through Bcl-2-EC-derived microvessels. Perfused glomeruli maintained podocin staining, but transmission electron microscopy revealed endothelial swelling and podocyte foot process effacement. Anastomosis of microvessels derived from Bcl-2-ECs with glomerular capillaries provides proof of concept that self-assembled microvessels can perfuse specialized organ structures such as glomeruli, but that perfusion alone may be insufficient to maintain normal structure.
Another Hard-Sphere Equation of State (1972)

A basic form for a Pade approximant of the hard-sphere equation of state is proposed. This form incorporates both high and low density limiting behavior and can easily be extended to include any further virial coefficients as they are calculated. Furthermore, addition of a simple term converts the approximant into an accurate equation for hard-sphere fluids. Hard-sphere solid behavior can also be estimated using a modification of the approximant, although the results are not as satisfactory as those of the high density hard-sphere equation of state.
Improved spectrophotometric assay for lytic polysaccharide monooxygenase (2019)

The biphenol hydrocoerulignone is a better substrate for LPMO than the monophenol 2,6-DMP, because of a ~ 30 times lower apparent <i>K</i> <sub>M</sub> value and a 160 mV lower oxidation potential. This greatly increases the measured LPMO activity when using hydrocoerulignone instead of 2,6-DMP under otherwise similar assay conditions. The improved activity allows the adaptation of the LPMO assay toward a higher sensitivity, different buffers and pH values, more stable assay conditions or to overcome low concentrations of inhibiting substances. The developed assay protocol and optimization guidelines increase the adaptability and applicability of the hydrocoerulignone assay for the production, purification, and characterization of LPMOs.
Electrochemical Conversion of Dichloroacetic Acid to Chloroacetic Acid in a Microfluidic Stack and in a Series of Microfluidic Reactors (2015)

Abstract The electrochemical conversion of dichloroacetic acid into chloracetic acid is performed in three different microdevices: a simple microfluidic cell; a microfluidic stack equipped with various electrode chambers in series and three microfluidic cells in series. The synthesis of chloracetic acid is performed successfully at low cell potentials without supporting electrolyte with high yields and selectivity under a single‐pass mode. Optimization of the productivity and of the final concentration of the target product is achieved by using a stack with two or three electrode chambers in series. Using three microreactors in series offers interesting new perspectives, including the opportunity to modulate the current density among the reactors chain in order to optimize the figures of merit of the overall process. The effect of various operating parameters such as the initial concentration of dichloroacetic acid, the flow rate and the current density are also presented in detail.
Total Synthesis of Doliculide, a Potent Cytotoxic Cyclodepsipeptide from the Japanese Sea Hare Dolabella auricularia (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTotal Synthesis of Doliculide, a Potent Cytotoxic Cyclodepsipeptide from the Japanese Sea Hare Dolabella auriculariaHiroyuki Ishiwata, Hiroki Sone, Hideo Kigoshi, and Kiyoyuki YamadaCite this: J. Org. Chem. 1994, 59, 17, 4712–4713Publication Date (Print):August 1, 1994Publication History Published online1 May 2002Published inissue 1 August 1994https://pubs.acs.org/doi/10.1021/jo00096a003https://doi.org/10.1021/jo00096a003research-articleACS PublicationsRequest reuse permissionsArticle Views578Altmetric-Citations40LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Total Synthesis and Configurational Assignment of (−)‐Dictyostatin, a Microtubule‐Stabilizing Macrolide of Marine Sponge Origin (2004)

Ein flexibler und modularer Ansatz führte zur konvergenten und hoch stereokontrollierten Synthese des Antimitotikums Dictyostatin. Diese erste Totalsynthese belegte auch die vollständige Stereochemie der Verbindung, deren Konformation der von Discodermolid (siehe Bild) stark ähnelt, und sollte für die Synthese nützlicher Mengen und gezielt entworfener Analoga anwendbar sein. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2001/2004/z460589_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Role of NADPH oxidase in the brain injury of intracerebral hemorrhage (2005)

The major risk factors for intracerebral hemorrhage (ICH) are hypertension and aging. A fundamental mechanism for hypertension- and aging-induced vascular injury is oxidative stress. We hypothesize that oxidative stress has a crucial role in ICH. To test our hypothesis, we used bacterial collagenase to produce ICH in wild-type C57BL/6 and gp91phox knockout (gp91phox KO) mice (deficient in gp91phox subunit of the superoxide-producing enzyme NADPH oxidase). All animals were studied at 20-35 weeks of age, resembling an older patient population. We found that collagenase produced less bleeding in gp91phox KO mice than wild-type mice. Total oxidative product was lower in gp91phox KO mice than in wild-type mice, both under basal conditions and after ICH. Consistent with the ICH volume, brain edema formation, neurological deficit and a high mortality rate was noted in wild-type but not in gp91phox KO mice. This ICH-induced brain injury in wild-type mice is associated with enhanced expression of the gp91phox subunit of NADPH oxidase. In conclusion, the oxidative stress resulting from activation of NADPH oxidase contributes to ICH induced by collagenase and promotes brain injury.
Performance analysis of coated [sup 238]PuO[sub 2] fuel particles compact for radioisotope heater units (2000)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation Jean-Michel Tournier, Mohamed S. El-Genk; Performance analysis of coated 238PuO2 fuel particles compact for radioisotope heater units. AIP Conf. Proc. 19 January 2000; 504 (1): 1458–1465. https://doi.org/10.1063/1.1290966 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAIP Publishing PortfolioAIP Conference Proceedings Search Advanced Search |Citation Search
Charge Build-up In Plasma Cleaning Process Before Wire-bonding (1997)

Charge build-up in plasma cleaning process has been investigated from the view points of the plasma uniformity, gas species and pressure, plasma exposure time and substrate (circuit board) dependence. The spatial distribution of plasma parameters in plasma cleaning was diagnosed using a Langmuir probe. The charge build-up was evaluated using metdnitrideioxidelsilicon (MNOS) capacitors and metdoxideisilicon (MOS) capacitors. Gases such as Ar, Ar+H2 and 02 have been investigated. It has been found that the spatial distribution of plasma parameters is uniform, and that the charge build-up in the plasma cleaning is negligibly small as far as test chips are placed without substrates. Use of substrates was found to increase the amount of the charge build-up. The charge build-up was found to depend on size, material and structure of the substrate. It was found that the plasma cleaning with substrates having a conductive surface film such as plated gold film resulted in considerable charge build-up. A model to explain these phenomena is discussed.
Microbial Transformation of (+)-10β,14-Dihydroxy-allo-aromadendrane and (-) -allo- Aromadendrone (1999)

The biotransformation of (+)-10β,14-dihydroxy- allo-aromadendrane 1 and (-)- allo-aromadendrone 2 by Beauvaria densa CMC 3240, Beauvaria bassiana ATCC 7159, Curvularia lunata 2380 and Rhizopus sp. (from grapes) is investigated.
High quality large area ELOG InP on silicon for photonic integration using conventional optical lithography (2014)

A simple method of growing large areas of InP on Si through Epitaxial Lateral Overgrowth (ELOG) is presented. Isolated areas of high quality InP suitable for photonic integration are grown in deeply etched SiO2 mask fabricated using conventional optical lithography and reactive ion etching. This method is particularly attractive for monolithically integrating laser sources grown on InP with Si/SiO2 waveguide structure as the mask. The high optical quality of multi quantum well (MQW) layers grown on the ELOG layer is promisingly supportive of the feasibility of this method for mass production.
CD1d-restricted type II NKT cells are sufficient for the down regulation of immunosurveilance against a nonregressor syngeneic colon carcinoma (2006)

Proc Amer Assoc Cancer Res, Volume 47, 2006

4879 

Despite the progress in understanding tumor immunosurveillance and advances in tumor immunotherapy, therapeutic immunity to cancer is still not an established modality of treatment. One of the reasons for the failure of antitumor immune response is the existence of mechanisms for tumor tolerance. In this context, different T cell subsets with suppressive function have been described, as the widely studied CD4+CD25+ T regulatory (Treg) cell. Another cell reported to suppress anti-tumor immune responses is the CD1d-restricted natural killer (NK) T cell. Our laboratory previously demonstrated the role of suppressive CD4+ CD1d-dependent CD4+ NKT cells both in an immunogenic regressor tumor model and in a nontransfected, nonregressor, syngeneic tumor model, the lung metastases of the CT26 colon carcinoma. The existence of suppressive NKT cells in the CT26 tumor model is confirmed by the observation that both CD4+ T cell-depleted mice and CD1d KO mice, which lack CD1d-restricted NKT cells, were significantly resistant to lung metastases. In the same tumor model, we have now excluded an additional suppressive role of the Treg cell, as the depletion of Treg cells did not protect the mice from tumor growth. We then investigated whether the regulatory NKT cell is the better characterized Vα14-Jα18+ (type I) NKT cell, whose activation has been shown to be beneficial in a number of tumor models, or is the different and less well characterized non-Vα14-Jα18+ (type II) NKT cell. Although CD1d KO mice were significantly protected from tumor growth, Jα18 KO mice, which lack the type I NKT cell, were not. Moreover, like NKT cell-intact wild type mice, but unlike CD1d KO mice, Jα18 KO mice do not have any tumor antigen-specific lytic activity in vivo. These results indicate that type II NKT cells, in the absence of type I NKT cells, are sufficient for the down regulation of the immunosurveillance and the cytotoxic immune response against the CT26 tumor. Taken together all the observations suggest that the immunosuppressive CD4+ NKT cell is the type II NKT cell. As confirmation of this conclusion, CD4+ T cell-depleted Jα18 KO mice were protected from tumor growth. Finally, these findings may distinguish the different roles of these two NKT cell subsets in the regulation of tumor immunity and resolve an apparent paradox regarding the function of NKT cells in tumor immunology.