gebhart/patrepeval huggingface dataset:
Supplementary Table 7 - 9 from Clusterin Is a Gene-Specific Target of microRNA-21 in Head and Neck Squamous Cell Carcinoma (2023)

&lt;p&gt;XLSX file - 137K, Supplementary Table 7. has-mir-21 gene target prediction (miRanda). Supplementary Table 8. has-mir-21 gene target prediction (TargetScan 4.2). Supplementary Table 9. has-mir-21 gene target prediction (PicTar).&lt;/p&gt;
In Vitro Pharmacology of a Novel Non-Peptide Angiotensin II-Receptor Antagonist, E4177 (1993)

E4177, 3-[(2’-carboxybiphenyl-4-yl)methyl]-2-cyclopropyl-7-methyl-3H-imidazo[4,5-6]pyridine, was characterized by in vitro autoradiography and by examining functional antagonism upon angiotensin II (Ang II)-induced contraction of isolated vessels. In rat adrenal cortex and liver, E4177 competitively inhibited the specific binding of 125I-[Sar1, Ile8]Ang II, with IC50 being (5.2±1.0) × 10-8 M for the adrenal cortex and (1.2±0.3) × 10-7 M for the liver. These IC50 values were similar to those for losartan, which showed an IC50 of (6.0±0.9) × 10-8 M for the adrenal cortex and (1.3±0.5) × 10-7 M for the liver. In contrast, E4177 and losartan had little effect on the binding to rat adrenal medulla where AT2-receptors predominate. These results indicate that E4177 is ΑΤ1-specific as is losartan. E4177 and losartan competitively antagonized the Ang II-induced contraction of human and rabbit arterial strips without any agonistic action. The obtained IC50 values indicated that E4177 was twice as potent as losartan in human arteries and three times more so in rabbit aortic strips. Responses to norepinephrine, serotonin, histamine or KC1 were not affected by E4177. In addition, E4177 (10-5 M) had no effect on angiotensin-converting enzyme activity. These data indicate that E4177 is a potent AT, Ang II-receptor antagonist that may be clinically useful for the treatment of cardiovascular diseases such as hypertension.
Sensing of inspiration events from speech: comparison of deep learning and linguistic methods (2023)

Respiratory chest belt sensor can be used to measure the respiratory rate and other respiratory health parameters. Virtual Respiratory Belt, VRB, algorithms estimate the belt sensor waveform from speech audio. In this paper we compare the detection of inspiration events (IE) from respiratory belt sensor data using a novel neural VRB algorithm and the detections based on time-aligned linguistic content. The results show the superiority of the VRB method over word pause detection or grammatical content segmentation. The comparison of the methods show that both read and spontaneous speech content has a significant amount of ungrammatical breathing, that is, breathing events that are not aligned with grammatically appropriate places in language. This study gives new insights into the development of VRB methods and adds to the general understanding of speech breathing behavior. Moreover, a new VRB method, VRBOLA, for the reconstruction of the continuous breathing waveform is demonstrated.
Encapsulation of Water-Insoluble Drugs in Poly(butyl cyanoacrylate) Nanoparticles (2009)

Hydrophobic drugs, loperamide and paclitaxel, were loaded in poly(butyl cyanoacrylate) nanoparticles by polymerization of n-butyl-2-cyanoacrylate in aqueous-organic media in the presence of a drug. The particles were stabilized by dextran 70,000 and poloxamer 188 or by 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-5000] sodium salt. It was shown that in the presence of dichloromethane, methanol or ethanol the encapsulation efficiency of loperamide in the nanoparticles reached 80%. Loading of paclitaxel was efficient only in the presence of the lipid. The organic solvents did not significantly influence the nanoparticle morphology or their physicochemical parameters. Thus produced poly(butyl cyanoacrylate) nanoparticles enabled delivery of loperamide across the blood-brain barrier, which was evidenced by the drug analgesic effect evaluated by the tail-flick test.
Requirements for Fuel Consumption Measurement for Emissions Regulations US07/10 and for Euro 4/5Design Criteria and Layout of the Fuel Measurement Systems (2007)

&lt;div class="htmlview paragraph"&gt;New heavy-duty diesel engine emission test regulations require even tougher emission limits verified with steady-state as well as transient test procedures, which are becoming increasingly important.&lt;/div&gt; &lt;div class="htmlview paragraph"&gt;The target of this paper is to describe the requirements and tasks for the fuel consumption measurement system and to discuss critical parameters which are essential for the layout of the fuel measurement system.&lt;/div&gt; &lt;div class="htmlview paragraph"&gt;The resulting fuel measurement system will be described and the performance ability will be demonstrated by measurement results and application examples.&lt;/div&gt; &lt;div class="htmlview paragraph"&gt;High absolute measurement accuracy of the fuel mass flow sensor, high fuel temperature control accuracy, appropriate fuel pressure control and fast time response of the complete fuel measurement system are all crucial factors needed to achieve repeatable specific fuel consumption values within 1 g/kWh and an overall time response of less than 300 ms. This allows the determination of the on-line exhaust mass flow required for exhaust emission modal analysis and for emission certification with partial flow dilution according to Euro 4/5 without using a CVS system.&lt;/div&gt;
ChemInform Abstract: Stereoselective Alkylation of Chiral α‐Nitro Keto Imine Dianions. Observations on the Role of Amide Bases. (1988)

Abstract The optimal conditions for a diastereoselective alkylation of chiral α‐nitro keto imines such as (I) are established to provide the products (III) which subsequently can be transformed in versatile 1‐nitrocyclohexenes as it is exemplified for (IIId) (→ (IV)).
Resilient Information Architecture Platform for Smart Systems (RIAPS): Case Study for Distributed Apparent Power Control (2018)

Maintaining voltage and frequency stability in an islanded microgrid is challenging, due to the low system inertia. In addition, islanded microgrids have limited generation capability, requiring that all DGs contribute proportionally to meet the system power consumption. This paper proposes a distributed control algorithm for optimal apparent power utilization in islanded microgrids. The developed algorithm improves system apparent power utilization by maintaining proportional power sharing among DGs. A decentralized platform called Resilient Information Architecture Platform for Smart Systems (RIAPS) is introduced that runs on processors embedded within the DGs. The proposed algorithm is fully implemented in RIAPS platform and validated on a real-time microgrid testbed.
Engineering of Phytase for Improved Activity at Low pH (2002)

For industrial applications in animal feed, a phytase of interest must be optimally active in the pH range prevalent in the digestive tract. Therefore, the present investigation describes approaches to rationally engineer the pH activity profiles of Aspergillus fumigatus and consensus phytases. Decreasing the negative surface charge of the A. fumigatus Q27L phytase mutant by glycinamidylation of the surface carboxy groups (of Asp and Glu residues) lowered the pH optimum by ca. 0.5 unit but also resulted in 70 to 75% inactivation of the enzyme. Alternatively, detailed inspection of amino acid sequence alignments and of experimentally determined or homology modeled three-dimensional structures led to the identification of active-site amino acids that were considered to correlate with the activity maxima at low pH of A. niger NRRL 3135 phytase, A. niger pH 2.5 acid phosphatase, and Peniophora lycii phytase. Site-directed mutagenesis confirmed that, in A. fumigatus wild-type phytase, replacement of Gly-277 and Tyr-282 with the corresponding residues of A. niger phytase (Lys and His, respectively) gives rise to a second pH optimum at 2.8 to 3.4. In addition, the K68A single mutation (in both A. fumigatus and consensus phytase backbones), as well as the S140Y D141G double mutation (in A. fumigatus phytase backbones), decreased the pH optima with phytic acid as substrate by 0.5 to 1.0 unit, with either no change or even a slight increase in maximum specific activity. These findings significantly extend our tools for rationally designing an optimal phytase for a given purpose.
In vivo performance evaluation of the innovamedica pneumatic ventricular assist device (2011)

We evaluated the in vivo performance of the Innovamedica pneumatic ventricular assist device (VAD), a new prototype of a simple, low-cost device for hospital circulatory support programs. We implanted the Innovamedica VAD in 6 sheep (weighing 55 to 91 kg). The inflow cannula was placed in the left ventricle, and the outflow cannula was anastomosed to the descending thoracic aorta. After heparinization (3mg/kg), we initiated the pump and monitored its hemodynamic performance for 6 hours. In a subsequent study we implanted the device for left ventricular support in two sheep for a 30-day period. We evaluated device performance based on implantation procedure, hemodynamic performance, and hematological impact. We monitored hematological and biochemical variables, and we assessed hemolysis. In the short-term experiments, the pumps maintained a mean blood flow of 4.4 ± 0.8 L/min. During support, mean arterial blood pressure was 76 ± 15 mmHg. The overall average concentration of plasma free hemoglobin was 5.11 ± 0.6 mg/dl compared with a baseline value of 4.52 ± 0.7 mg/dl. In the 30 day trials, mean blood output was 4 l/min ± 0.2, plasma free hemoglobin was 5.9 ± 4 mg/dl for the 30 day period excluding the first 48 hours. Warfarin/Aspirin anticoagulation was used after the first 72 hours with an average INR of 2.9 for the entire test period. Post-mortem showed no blood clots or any significant tissue damage to brain, lungs or kidneys. The devices operated without any significant adverse events in all of the experiments. The Innovamedica VAD was easy to implant and de-air and was found to be effective, reliable and biocompatible.
Comparing In Vitro and In Vivo Activity of 2′-O-[2-(Methylamino)-2-oxoethyl]- and 2′-O-Methoxyethyl-Modified Antisense Oligonucleotides (2008)

A number of 2'- O-modified antisense oligonucleotides have been reported for their potential use in oligonucleotide-based therapeutics. To date, most of the in vivo data has been generated for 2'-O-MOE (2'-O-methoxyethyl)- and 2'-O-Me (2'-O-methyl)-modified ASOs (antisense oligonucleotides). We now report the synthesis and biological activity of another 2'-O-modification, namely 2'-O-[2-(methylamino)-2-oxoethyl] (2'-O-NMA). This modification resulted in an increase in the affinity of antisense oligonucleotides to complementary RNA similar to 2'-O-MOE-modified ASOs as compared to first-generation antisense oligodeoxynucleotides. The ASO modified with 2'-O-NMA reduced expression of PTEN mRNA in vitro and in vivo in a dose-dependent manner similar to 2'-O-MOE modified ASO. Importantly, toxicity parameters such as AST, ALT, organ weights, and body weights were found to be normal similar to 2'-O-MOE ASO-treated animal models. The data generated in these experiments suggest that 2'-O-NMA is a useful modification for potential application in both antisense and other oligonucleotide-based drug discovery efforts.
Guanidium iodide treatment of size-controlled CsPbI3 quantum dots for stable crystal phase and highly efficient red LEDs (2023)

The optoelectronic properties of lead-halide perovskite quantum dots (QDs) can be tuned according to their halogen composition ratio and quantum size effect. However, the pure red emission of CsPbI3 QDs exhibits poor phase stability, thereby resulting in a phase transition to a nonphotoactive yellow phase at room temperature. These phase instabilities are accompanied by crystal strain and instability of the QD surface, which induce ion diffusion and desorption. Herein, we demonstrate the precise size control and postsynthetic guanidinium iodide (GAI) treatment to stabilize the crystal phase of CsPbI3 QDs. These synergistic effect results in higher ambient photoluminescence stability and photostability than those of pristine QDs. More importantly, the CsPbI3 QD light-emitting diodes achieve excellent external quantum efficiency of 22.5% and longer operational stability of over 10 h by the synergy effect of QD size control and GAI treatment.
Search for third-generation scalar leptoquarks in the tτ channel in proton-proton collisions at s = 8 $$ \sqrt{s}=8 $$ TeV (2015)

A search for pair production of third-generation scalar leptoquarks decaying to top quark and lepton pairs is presented using proton-proton collision data at a center-ofmass energy of s = 8 TeV collected with the CMS detector at the LHC and corresponding to an integrated luminosity of 19.7 fb -1 . The search is performed using events that contain an electron or a muon, a hadronically decaying lepton, and two or more jets. The observations are found to be consistent with the standard model predictions. Assuming that all leptoquarks decay to a top quark and a lepton, the existence of pair produced, charge -1/3, third-generation leptoquarks up to a mass of 685 GeV is excluded at 95% confidence level. This result constitutes the first direct limit for leptoquarks decaying into a top quark and a lepton, and may also be applied directly to the pair production of bottom squarks decaying predominantly via the R-parity violating coupling 333 .
Mixup Inference: Better Exploiting Mixup to Defend Adversarial Attacks (2019)

It has been widely recognized that adversarial examples can be easily crafted to fool deep networks, which mainly root from the locally non-linear behavior nearby input examples. Applying mixup in training provides an effective mechanism to improve generalization performance and model robustness against adversarial perturbations, which introduces the globally linear behavior in-between training examples. However, in previous work, the mixup-trained models only passively defend adversarial attacks in inference by directly classifying the inputs, where the induced global linearity is not well exploited. Namely, since the locality of the adversarial perturbations, it would be more efficient to actively break the locality via the globality of the model predictions. Inspired by simple geometric intuition, we develop an inference principle, named mixup inference (MI), for mixup-trained models. MI mixups the input with other random clean samples, which can shrink and transfer the equivalent perturbation if the input is adversarial. Our experiments on CIFAR-10 and CIFAR-100 demonstrate that MI can further improve the adversarial robustness for the models trained by mixup and its variants.
Surfactant-Assisted Direct Crystallization of CON-Type Zeolites with Particle Size and Acid-Site Location Controlled (2022)

CON-type boroaluminosilicate zeolites with varying Si/Al ratios ([A, B]-CON-x-C; x: Si/Al ratio; x = 200–600) were synthesized via a direct crystallization method from a synthetic gel containing cetyltrimethylammonium bromide (CTAB). We confirmed that the [Al, B]-CON-x-C zeolites obtained from the CTAB-containing gel exhibited smaller particles and almost twice the external surface area compared to those of the [Al, B]-CON-x zeolites crystalized from gels that did not contain CTAB, suggesting a considerable influence of CTAB on the crystallization process. In the methanol-to-olefin (MTO) reaction, [Al, B]-CON-x-C zeolites showed a longer catalyst lifetime compared to the corresponding [Al, B]-CON-x zeolites, together with a characteristic high propylene selectivity (∼50%). Systematic investigations using 27Al, 29Si, and 11B magic-angle spinning (MAS) NMR as well as 27Al 3QMQMAS NMR spectroscopy, together with Fourier transform infrared (FT-IR) spectroscopy with CO as the probe molecule, confirmed the negligible impact of CTAB on the local structure of [Al, B]-CON zeolites. On the other hand, a lower amount of Al and related Brønsted-acid sites on the external surface of [Al, B]-CON-x-C zeolites relative to the corresponding [Al, B]-CON-x zeolites was confirmed by FT-IR spectroscopy using quinoline (Qu) as the probe molecule. A systematic investigation of the catalytic MTO reaction, together with the structural, physicochemical, and spectroscopic analyses unveiled a dual role for CTAB during the crystallization process, i.e., control over the particle size and over the location of Al sites within the particles, which contributes to prolonging the MTO catalytic lifetime without changing the acidic properties and undermining the propylene selectivity.
18F- and 68Ga-Labeled Neurotensin Peptides for PET Imaging of Neurotensin Receptor 1 (2016)

The neurotensin (NT) receptor-1 (NTS1) is overexpressed in a variety of carcinomas and is therefore an interesting target for imaging with positron emission tomography (PET). The aim of this study was the development of new NT derivatives based on the metabolically stable peptide sequence NLys-Lys-Pro-Tyr-Tle-Leu suitable for PET imaging. The NT peptides were synthesized by solid-phase supported peptide synthesis and elongated with respective chelators (NODA-GA, DOTA) for (68)Ga-labeling or propargylglycine for (18)F-labeling via copper-catalyzed azide-alkyne cycloaddition. Receptor affinities of the peptides for NTS1 were in the range of 19-110 nM. Biodistribution studies using HT29 tumor-bearing mice showed highest tumor uptake for [(68)Ga]6 and [(68)Ga]8 and specific binding in small-animal PET studies. The tumor uptake of (68)Ga-labeled peptides in vivo significantly correlated with the in vitro Ki values for NTS1. [(68)Ga]8 displayed an excellent tumor-to-background ratio and could therefore be considered as an appropriate molecular probe for NTS1 imaging by PET.
Microvascular Structure and Network in the Retina of Patients With Ischemic Stroke (2013)

Patients with ischemic stroke have a sparser and more tortuous microvascular network in the retina. These findings provide insight into the structure and pattern of microcirculation changes in stroke.
Data from CCR5 Governs DNA Damage Repair and Breast Cancer Stem Cell Expansion (2023)

&lt;div&gt;Abstract&lt;p&gt;The functional significance of the chemokine receptor CCR5 in human breast cancer epithelial cells is poorly understood. Here, we report that CCR5 expression in human breast cancer correlates with poor outcome. CCR5&lt;sup&gt;+&lt;/sup&gt; breast cancer epithelial cells formed mammospheres and initiated tumors with &gt;60-fold greater efficiency in mice. Reintroduction of CCR5 expression into CCR5-negative breast cancer cells promoted tumor metastases and induced DNA repair gene expression and activity. CCR5 antagonists Maraviroc and Vicriviroc dramatically enhanced cell killing mediated by DNA-damaging chemotherapeutic agents. Single-cell analysis revealed CCR5 governs PI3K/Akt, ribosomal biogenesis, and cell survival signaling. As CCR5 augments DNA repair and is reexpressed selectively on cancerous, but not normal breast epithelial cells, CCR5 inhibitors may enhance the tumor-specific activities of DNA damage response–based treatments, allowing a dose reduction of standard chemotherapy and radiation.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Significance:&lt;/b&gt; This study offers a preclinical rationale to reposition CCR5 inhibitors to improve the treatment of breast cancer, based on their ability to enhance the tumor-specific activities of DNA-damaging chemotherapies administered in that disease. &lt;i&gt;Cancer Res; 78(7); 1657–71. ©2018 AACR&lt;/i&gt;.&lt;/p&gt;&lt;/div&gt;
Influence of sex steroids on development of cultured fetal rat metatarsal bones. (1997)

The effects of 17 beta-estradiol (E), dihydrotestosterone (D, a non aromatisable androgen), and progesterone (P) on osteogenesis were studied on fetal rat cartilaginous anlagues cultivated in vitro. The three medial metatarsal rudiments were harvested at day 19 of gestation and grown in 1% BSA MEM medium (MO 643, Sigma) without serum nor antibiotics. After a 18h preincubation period, hormones were added for 8 days. Paired controls were incubated in the same volume of medium. The length, the metacarpal thickness and the size of the mineralized zone were measured every day, using a calibrated eyepiece (magnification X 40). DNA and protein synthesis, cartilage metabolism and mineralization were evaluated by monitoring the incorporation of 3H-Thymidine, 3H-Proline, 35S and 45Ca into anlagues for the last three hours of incubation, respectively. The dose/response effect of each steroid was studied at the concentrations of 10(-4) M, 10(-6) M, 10(-7) M and 10(-9) M. No difference was observed between male and female fetuses. A significant positive effect on total length (% of length measured at harvesting day) was observed with the 10(-7) M dose of E (163% +/- 2 vs 148% +/- 4 in controls) or D (158% +/- 3). Endochondral growth was not modified by P treatment. The effect of the three steroids (given at a dose of 10(-7) M) alone or as combinations (E, D, P, EP, ED, PD, EPD) confirmed the positive effect of E on endochondral growth and, to a lesser extend, of D and the association ED. Nevertheless, D had a better effect than E on endomembranous growth. On the contrary, P did not affect growth neither administrated alone nor in combination with E or D, while a positive effect of P on mineralization was demonstrated. The treatment associating the three steroids slowed down all the parameters concerning growth but strongly stimulated calcification.