gebhart/patrepeval huggingface dataset:
Optimal procedure for extracting RNA from human ocular tissues and expression profiling of the congenital glaucoma gene FOXC1 using quantitative RT-PCR. (2001)

To develop methods for obtaining high quality RNA from human donor eyes and to determine the expression profile of the congenital glaucoma gene FOXC1 in human ocular tissues.To obtain high quality RNA from donor eyes, several different preservation methods were tested including storing eyes on ice, freezing eyes, and placing eyes in the commercial fixative RNAlaterTM prior to dissection and RNA extraction. Nine different ocular tissues from human donors were dissected and examined. Pigment-free total RNA was isolated and used for quantitative real-time RT-PCR using FOXC1 and GAPDH (internal standard) primers to assess the quality and expression of FOXC1.An expression profile of FOXC1 in human ocular tissues was determined using quantitative PCR of RNA isolated using a simple and effective procedure for ocular tissue preservation and pigment-free RNA isolation. Higher quality RNA was obtained from human donor eyes preserved in RNAlaterTM compared to RNA extracted from eyes stored on ice or frozen at -80 degrees C. RNA extraction techniques that removed interfering pigment from ocular tissues produced RNA that could be easily amplified by PCR. In the adult human eye, expression of FOXC1 was greatest in the trabecular meshwork (TM) followed by the optic nerve head, choroid/RPE, ciliary body, cornea, and iris. FOXC1 expression levels were much lower in other non-ocular human tissues, such as liver, muscle, lung, heart, and kidney.Using an optimized donor eye preservation method and tissue RNA isolation procedure, we show that the FOXC1 transcription factor gene, which is known to be associated with developmental glaucoma, also may have an important role in the adult eye.
Understanding Organizational Reforms in the Modern State: Specialization and Integration in Norway and France (2012)

This article examines the challenge Norway and France face in coordinating specialized government activities after 10 years of comprehensive reforms. The focus is on the tension between territorial and sectoral specialization and between vertical and horizontal specialization. We describe both sector‐specific administrative reforms and more overarching general reforms, looking at similarities and differences in the reorganization choices made by the two countries and also at what drives change. We argue that a combination of factors is required to explain outcomes. These factors include not only home‐grown reforms but also sectoral challenges, diffusion and learning from abroad, adaptation to the financial crisis and budget deficit, and choices made by powerful political executives. Sometimes these factors work together and reinforce each other, producing radical reforms; at other times they have a mutually constraining influence, resulting in only minor changes.
Nasogastric Tube Perforation of the Nasopharynx (1985)

We have presented two unusual cases of nasogastric tube perforation of the posterior nasopharynx. Complications included subcutaneous emphysema, pneumomediastinum, and pleural effusion. Both patients recovered. Recommendations to improve nasogastric tube safety include gentle placement using lubricant and an appropriately sized tube, confirmation of tube placement before any infusion, and awareness of patients at increased risk of mucosal injury.
The Notion of Security for Probabilistic Cryptosystems (1988)

Three very different formal definitions of security for public-key cryptosystems have been proposed—two by Goldwasser and Micali and one by Yao. We prove all of them to be equivalent. This equivalence provides evidence that the right formalization of the notion of security has been reached.
Influence of doping with alkaline earth metals on the optical properties of thermochromic VO2 (2015)

Thin films of doped VO2 were deposited, analyzed, and optimized with regard to their solar energy transmittance (Tsol) and visible/luminous light transmittance (Tlum) which are important parameters in the context of smart window applications in buildings. The doping with alkaline earth metals (AEM) like Mg, Ca, Sr, or Ba increased both Tsol and Tlum due to a bandgap widening and an associated absorption edge blue-shift. Thereby, the brown-yellowish color impression of pure VO2 thin films, which is one major hindrance limiting the usage of VO2 as thermochromic window coating, was overcome. Transparent thin films with excellent switching behavior were prepared by sputtering. Highly doped V1−xMexO2 (Me = Ca, Sr, Ba) kept its excellent thermochromic switching behavior up to x(Me) = Me/(Me + V) = 10 at. % doping level, while the optical bandgap energy was increased from 1.64 eV for undoped VO2 to 2.38 eV for x(Mg) = 7.7 at. %, 1.85 eV for x(Ca) = 7.4 at. %, 1.84 eV for x(Sr) = 6.4 at. % and 1.70 eV for x(Ba) = 6.8 at. %, as well as the absorption edge is blue shifted by increasing AEM contents. Also, the critical temperature ϑc, at which the semiconductor-to-metal transition (SMT) occurs, was decreased by AEM doping, which amounted to about −0.5 K/at. % for all AEM on average. The critical temperature was determined by transmittance-temperature hysteresis measurements. Furthermore, Tsol and Tlum were calculated and were found to be significantly enhanced by AEM doping. Tlum increased from 32.0% in undoped VO2 to 43.4% in VO2 doped with 6.4 at. % Sr. Similar improvements were found for other AEM. The modulation of the solar energy transmittance ΔTsol, which is the difference of the Tsol values in the low and high temperature phase, was almost constant or even slightly increased when the doping level was increased up to about 10 at. % Ca, Sr, or Ba.
Cepstrum-based pitch detection using a new statistical V/UV classification algorithm (1999)

An improved cepstrum-based voicing detection and pitch determination algorithm is presented. Voicing decisions are made using a multifeature voiced/unvoiced classification algorithm based on statistical analysis of cepstral peak, zero-crossing rate, and energy of short-time segments of the speech signal. Pitch frequency information is extracted by a modified cepstrum-based method and then carefully refined using pitch tracking, correction, and smoothing algorithms. Performance analysis on a large database indicates considerable improvement relative to the conventional cepstrum method. The proposed algorithm is also shown to be robust to additive noise.
Glycosylated molecules secreted by human middle ear mucosa in culture (1990)

Conference Article| December 01 1990 Glycosylated molecules secreted by human middle ear mucosa in culture DAVID A. HUTTON; DAVID A. HUTTON *Department of Physiological Sciences, University of Newcastle-upon-Tyne, NE2 4HH, U.K. Search for other works by this author on: This Site PubMed Google Scholar JOHN HILL; JOHN HILL †Ear, Nose and Throat Department, Freeman Hospital, Newcastle-upon-Tyne NE2 4HH, U.K. Search for other works by this author on: This Site PubMed Google Scholar GARY G. R. GREEN; GARY G. R. GREEN *Department of Physiological Sciences, University of Newcastle-upon-Tyne, NE2 4HH, U.K. Search for other works by this author on: This Site PubMed Google Scholar JOHN P. BIRCHALL; JOHN P. BIRCHALL †Ear, Nose and Throat Department, Freeman Hospital, Newcastle-upon-Tyne NE2 4HH, U.K. Search for other works by this author on: This Site PubMed Google Scholar JEFFREY P. PEARSON JEFFREY P. PEARSON *Department of Physiological Sciences, University of Newcastle-upon-Tyne, NE2 4HH, U.K. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1990) 18 (6): 1277. https://doi.org/10.1042/bst0181277 Article history Received: June 18 1990 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Cite Icon Cite Get Permissions Citation DAVID A. HUTTON, JOHN HILL, GARY G. R. GREEN, JOHN P. BIRCHALL, JEFFREY P. PEARSON; Glycosylated molecules secreted by human middle ear mucosa in culture. Biochem Soc Trans 1 December 1990; 18 (6): 1277. doi: https://doi.org/10.1042/bst0181277 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1990 Biochemical Society1990 Article PDF first page preview Close Modal You do not currently have access to this content.
Long-Term Stabilization and Operation of a Soliton Micro-Comb for 9-Days (2019)

We report the long-term stabilization of a soliton micro-comb over 9 days of continuous operation. Using an integrated heater, the original pump-cavity detuning is maintained with a simple active feedback method. © 2019 The Author(s)
Rapid screening of PEM FC cathode catalysts (2009)

One of the major bottlenecks in catalyst development for proton exchange membrane fuel cell (PEMFC) is the lack of fast high-throughput testing methods. Fast screening techniques enable a large number of catalysts to be tested in a relatively short time under the same conditions.&#13;\n&#13;\nThis project was focused on developing systems for screening catalysts used for the oxygen reduction reaction (ORR) at the cathode of PEMFCs. The first system developed was the 64 channel pin electrode array, using liquid electrolyte. The developed method improved both the quality and reproducibility of the data and has been used to rank catalyst samples, as well as to optimize loadings and the preparative methods of inks. The second system developed was a 25 channel array fuel cell, which operated under conditions analogous to real fuel cell environments. Both methods allowed trends in characteristics and activities of a series of catalysts to be established more rapidly than individual single-electrode methods such as half cell, rotating disc electrode (RDE) or fuel cell. The results from the two high-throughput methods are compared to those of the single channel systems. The mass and specific activities towards reduction of oxygen were studied using a series of Pt/C and PtCo/C catalysts. The catalytic properties of the Pt based carbon-supported catalysts were related to their structure e.g. particle size and lattice parameter, which were obtained mainly using Xray diffraction (XRD). It was found that the results acquired sing parallel screening methods were similar to those collected with a RDE and a fuel cell.&#13;\n&#13;\nThe thesis concludes with suggestions regarding the future improvement/development of high-throughput techniques. For the 64 channel array system the problem associated with the corrosion of the components should be solved. Similarly, the major changes for the array fuel cell would be to modify a heating system and further development of the anode flow field.
Multilevel Object Security Model. (1986)

Abstract : This report is a summary of the Multilevel Object Security Model (MOSM) project. The MOSM effort involved the development of an intermediate-level security model which satisfies the requirements of the Bell-Lapadula model and the NRL model, while incorporating the ease of verification of the Bell-Lapadula model and the flexibility of the user interface of the NRL model. The project consisted of three tasks: development of the model, an abstract implementation of the model in terms of the NRL model, and an analysis of the Diamond Document Handling system with respect to the model. Each of these tasks is described in detail and a formal statement of the MOSM appears in the appendix. Keywords: Computer security; Multilevel security; Formal security model.
Investigation of the Biopharmaceutical Behavior of Theophylline Hydrophilic Matrix Tablets Using USP Methods and an Artificial Digestive System (2007)

This work aimed to investigate the biopharmaceutical behavior of hydrophilic matrix tablets of theophylline using different in vitro methods: USP II, USP IV, and a novel in vitro system simulating the gastrointestinal tract in man called the artificial digestive system (ADS). The potentiality of each method was evaluated by establishing in vitro/in vivo correlation. Using USP methods, the drug release was pH-independent and dependent on agitation intensity. Level A IVIVCs could be established using the different in vitro methods but one to one correlation was established only when the ADS method was used. For the prediction of in vivo drug dosage form behavior based on in vitro methods, the ADS showed a high predictability when compared to USP in vitro methods.
Mobile Standards-Based Traffic Light Detection in Assistive Devices for Individuals with Color-Vision Deficiency (2014)

Considering the substantial population affected by some form of color-vision deficiency (CVD), reliable traffic control signal head light detection is an important problem for driver-assistance systems. While a large number of technologies can be used to localize traffic lights, without drastic changes in infrastructure, only visual information can be used in identifying the status of the light. In addition, traffic light detection is not currently integrated into any driver-assistance systems, making driving for individuals with CVD (where permitted) dangerous to other drivers, pedestrians, and themselves. This paper presents a robust, traffic-standards-based, and computationally efficient method for detecting the status of the traffic lights without relying on Global Positioning System, lidar, radar information, or prior (map-based) knowledge. To the extent of our knowledge, this is the first work to use official Institute of Transportation Engineers (U.S.) and British Standards Institute (European Union) standards for defining traffic light colors, as well as integrating a number of fail-safe mechanisms designed to prevent erroneous detection. The algorithm can be easily ported over to an embedded smart camera platform and used as a windshield-mounted driver-assistance device by individuals with CVD. The system can accurately identify the status of the light at 400 ft away from the intersection, reliably detecting solid, faulty, arrow, and high-visibility signal lights. Over 50 h of video (over 2000 intersections) were tested with the system, containing intersections with one to four traffic lights, governing different lanes of traffic, with 97.5% accuracy of solid light detection.
Highland Barley Tea Polyphenols Extract Alleviates Skeletal Muscle Fibrosis in Mice by Reducing Oxidative Stress, Inflammation, and Cell Senescence (2022)

The tea of roasted Highland barley is a cereal-based drink rich in polyphenols. A model of skeletal muscle senescence and fibrosis was constructed using d-galactose-induced C2C12 myotubes, and Highland barley tea Polyphenols (HBP) were extracted for the intervention. We found that HBP effectively alleviated oxidative stress, inflammation, and fibrosis induced by d-galactose-induced skeletal muscle senescence. Also, HBP treatment significantly down-regulated pro-fibrotic genes, inflammation, and oxidative stress levels in a contusion model of senescent mice. Reduced levels of SIRT3 protein was found to be an essential factor in skeletal muscle senescence and fibrosis in both cellular and animal models, while HBP treatment significantly increased SIRT3 protein levels and viability in skeletal muscle. The ability of HBP to mitigate skeletal muscle fibrosis and oxidative stress was significantly reduced after <i>SIRT3</i> silencing. Together, these results suggest that HBP intervention can significantly alleviate aging-induced oxidative stress, inflammation, and skeletal muscle fibrosis, with the activation of <i>SIRT3</i> as the underlying mechanism of action.
Crystalline InGaZnO quaternary nanowires with superlattice structure for high-performance thin-film transistors (2019)

Amorphous indium—gallium—zinc oxide (a-IGZO) materials have been widely explored for various thin-film transistor (TFT) applications; however, their device performance is still restricted by the intrinsic material issues especially due to their non-crystalline nature. In this study, highly crystalline superlattice-structured IGZO nanowires (NWs) with different Ga concentration are successfully fabricated by enhanced ambient-pressure chemical vapor deposition (CVD). The unique superlattice structure together with the optimal Ga concentration (i.e., 31 at.%) are found to effectively modulate the carrier concentration as well as efficiently suppress the oxygen vacancy formation for the superior NW device performance. In specific, the In1.8Ga1.8Zn24O7 NW field-effect transistor exhibit impressive device characteristics with the average electron mobility of ~ 110 cm2·V−1·s−1 and on/off current ratio of ~ 106. Importantly, these NWs can also be integrated into NW parallel arrays for the construction of high-performance TFT devices, in which their performance is comparable to many state-of-the-art IGZO TFTs. All these results can evidently indicate the promising potential of these crystalline superlattice-structured IGZO NWs for the practical utilization in next-generation metal-oxide TFT device technologies.
Nanowiring light (2005)

Recent advances in the fabrication and manipulation of sub-wavelength optical fibers provide new methods for building chemical and biological sensors, generating supercontinuum light by nonlinear pulse propagation, and constructing microphotonic components and devices. We examine the properties of sub-wavelength dimension optical fibers as waveguides. The reported silica submicrometer- or nanometer-diameter wires are suitable for low-loss optical wave guiding. Thanks to their excellent uniformity, large length, high flexibility, and strength, these wires can be manipulated and assembled with high accuracy.
Imido Alkylidene Bispyrrolyl Complexes of Tungsten (2007)

We have prepared tungsten bispyrrolyl (Pyr) or bis-2,5-dimethylpyrrolyl (Me&lt;sub&gt;2&lt;/sub&gt;Pyr) complexes W(NAr)(CHCMe&lt;sub&gt;2&lt;/sub&gt;Ph)(η&lt;sup&gt;1&lt;/sup&gt;-Pyr)&lt;sub&gt;2&lt;/sub&gt;(DME) (&lt;b&gt;1&lt;/b&gt;), W(NAr)(CHCMe&lt;sub&gt;2&lt;/sub&gt;Ph)(η&lt;sup&gt;1&lt;/sup&gt;-Me&lt;sub&gt;2&lt;/sub&gt;Pyr)(η&lt;sup&gt;5&lt;/sup&gt;-Me&lt;sub&gt;2&lt;/sub&gt;Pyr) (&lt;b&gt;2&lt;/b&gt;), W(NAr&lt;sub&gt;Cl&lt;/sub&gt;)(CHCMe&lt;sub&gt;3&lt;/sub&gt;)(η&lt;sup&gt;1&lt;/sup&gt;-Pyr)&lt;sub&gt;2&lt;/sub&gt;(DME) (&lt;b&gt;3b&lt;/b&gt;), and W(NAr&lt;sub&gt;Cl&lt;/sub&gt;)(CHCMe&lt;sub&gt;3&lt;/sub&gt;)(η&lt;sup&gt;1&lt;/sup&gt;-Me&lt;sub&gt;2&lt;/sub&gt;Pyr)(η&lt;sup&gt;5&lt;/sup&gt;-Me&lt;sub&gt;2&lt;/sub&gt;Pyr) (&lt;b&gt;4&lt;/b&gt;) (Ar = 2,6-diisopropylphenyl,\nAr&lt;sub&gt;Cl&lt;/sub&gt; = 2,6-dichlorophenyl) in excellent yields by treating the appropriate W(NR)(CHCMe&lt;sub&gt;2&lt;/sub&gt;R‘)(OTf)&lt;sub&gt;2&lt;/sub&gt;(DME) species with LiPyr or LiMe&lt;sub&gt;2&lt;/sub&gt;Pyr. Compounds &lt;b&gt;2&lt;/b&gt; and &lt;b&gt;4&lt;/b&gt; react with ethylene slowly to yield stable\nmethylene complexes, W(NAr)(CH&lt;sub&gt;2&lt;/sub&gt;)(η&lt;sup&gt;1&lt;/sup&gt;-Me&lt;sub&gt;2&lt;/sub&gt;Pyr)(η&lt;sup&gt;5&lt;/sup&gt;-Me&lt;sub&gt;2&lt;/sub&gt;Pyr) (&lt;b&gt;5&lt;/b&gt;) and W(NAr&lt;sub&gt;Cl&lt;/sub&gt;)(CH&lt;sub&gt;2&lt;/sub&gt;)(η&lt;sup&gt;1&lt;/sup&gt;-Me&lt;sub&gt;2&lt;/sub&gt;Pyr)(η&lt;sup&gt;5&lt;/sup&gt;-Me&lt;sub&gt;2&lt;/sub&gt;Pyr) (&lt;b&gt;6&lt;/b&gt;). In contrast, treatment of what is believed to be a fortuitous 1:1 mixture of &lt;b&gt;3b&lt;/b&gt; and\n[W(NAr&lt;sub&gt;Cl&lt;/sub&gt;)(CHCMe&lt;sub&gt;3&lt;/sub&gt;)(η&lt;sup&gt;1&lt;/sup&gt;-Pyr)&lt;sub&gt;2&lt;/sub&gt;][W(NAr&lt;sub&gt;Cl&lt;/sub&gt;)(CHCMe&lt;sub&gt;3&lt;/sub&gt;)(η&lt;sup&gt;1&lt;/sup&gt;-Pyr)(η&lt;sup&gt;5&lt;/sup&gt;-Pyr)] with ethylene leads to formation\nof [W(μ-NAr&lt;sub&gt;Cl&lt;/sub&gt;)(η&lt;sup&gt;1&lt;/sup&gt;-Pyr)&lt;sub&gt;2&lt;/sub&gt;]&lt;sub&gt;2&lt;/sub&gt; (&lt;b&gt;7&lt;/b&gt;) in moderate (54%) yield. Compounds &lt;b&gt;2&lt;/b&gt; and &lt;b&gt;4&lt;/b&gt; react with [HNMe&lt;sub&gt;2&lt;/sub&gt;Ph][B(Ar&lt;sub&gt;F&lt;/sub&gt;)&lt;sub&gt;4&lt;/sub&gt;] (Ar&lt;sub&gt;F&lt;/sub&gt; = 3,5-(CF&lt;sub&gt;3&lt;/sub&gt;)&lt;sub&gt;2&lt;/sub&gt;C&lt;sub&gt;6&lt;/sub&gt;H&lt;sub&gt;3&lt;/sub&gt;) in dichloromethane to yield cationic species that contain one η&lt;sup&gt;5&lt;/sup&gt;-Me&lt;sub&gt;2&lt;/sub&gt;Pyr ligand and one 2,5-dimethylpyrrolenine ligand formed through addition of a proton to C(2) of a\ndimethylpyrrolyl ligand, e.g., [W(NAr&lt;sub&gt;Cl&lt;/sub&gt;)(CHCMe&lt;sub&gt;3&lt;/sub&gt;)(Me&lt;sub&gt;2&lt;/sub&gt;Pyr){NC&lt;sub&gt;4&lt;/sub&gt;(H&lt;sub&gt;3&lt;/sub&gt;-2,3,4)(Me&lt;sub&gt;2&lt;/sub&gt;-2,5)}]&lt;sup&gt;+&lt;/sup&gt;[B(Ar&lt;sub&gt;F&lt;/sub&gt;)&lt;sub&gt;4&lt;/sub&gt;]&lt;sup&gt;-&lt;/sup&gt; (&lt;b&gt;9&lt;/b&gt;).\nX-ray studies were carried out on &lt;b&gt;1&lt;/b&gt;, &lt;b&gt;2&lt;/b&gt;, &lt;b&gt;6&lt;/b&gt;, &lt;b&gt;7&lt;/b&gt;, and &lt;b&gt;9&lt;/b&gt;.
Monolithic photonic crystal fiber acoustic sensor (2017)

This paper describes an acoustic sensor based on a single-crystal silicon diaphragm with an integrated Photonic Crystal (PC) mirror, whose deflection is measured in a Fabry-Perot resonator formed between the PC mirror and a gold coated single-mode fiber. The sensors are fabricated on standard silicon wafers, yielding monolithic, low-stress sensing diaphragms. The packaged sensor exhibits a minimum detectable pressure of 17μPa/√Hz in the 8.5kHz to 18kHz frequency range.
Effects of camptothecin on tumor cell proliferation and angiogenesis when coupled to a bombesin analog used as a targeted delivery vector (2007)

The camptothecin-bombesin conjugate termed DC-51-43, as a novel targeted drug delivery system, was examined in over 10 human tumor cell lines and shows a potent antiproliferative activity. This conjugate has also demonstrated its antitumor activity in our previous experiments. In our present study, we evaluate this conjugate for its antiangiogenic activity by in-vitro and in-vivo experiments. The camptothecin-bombesin conjugate and free camptothecin show potent in-vitro inhibitory activities of cell adhesion to various extracellular matrix components and integrins alphaVbeta3 and alphaVbeta5, not beta1/alphabeta1. This conjugate displays inhibitory activity to cell migration and invasion at concentrations of 10 micromol/l or above. This conjugate is also effective against in-vitro capillary-like tube formation of endothelial cells (at 40 micromol/l), and in-vivo angiogenesis as seen by blocking the spread of host mice endothelial cells into matrigel plugs. These experimental results support the fact that the camptothecin-bombesin conjugate has therapeutic activities against angiogenesis. By binding to bombesin receptor-expressing sites, this bombesin analog, consisting of 11 amino acids, is potentially a novel delivery vector for nonspecific cytotoxic agents.
ChemInform Abstract: TETRACYANOETHYLENE ADDITION TO IRON TRICARBONYL COMPLEXES OF SUBSTITUTED CYCLOOCTATETRAENES, REGIOSELECTIVITY CONSIDERATIONS DURING FORMATION OF THE 2,3,4,10‐TETRAHAPTO ADDUCTS (1975)

Abstract Die Cycloaddition von Tetracyanäthylen (II) an eine Anzahl von Cyclooctatetraen‐Fetricarbonyle wie (I) führt in einem ungewöhnlichen lß‐Bindungsprozeß zur Bildung von n4‐Produkten wie (III), in denen das Fe‐Atom sowohl 0‐ als auch n‐gebunden ist.
Surgical and Hormonal Therapy for Cryptorchidism: An Overview (1988)

The treatment of the undescended testis still engenders controversy on both sides of the Atlantic. To date, there is still no consensus on how to treat a child with an undescended testis. Some physicians advocate surgical therapy of all children whose testes are not located at the bottom of the scrotum whereas others feel that hormonal therapy should be tried initially on all such boys. The following attempts to provide an objective assessment of each of these two therapeutic modalities, highlighting the current controversies.