gebhart/patrepeval huggingface dataset:
Catalytic and noncatalytic nucleotide binding sites of the Escherichia coli F1 ATPase Amino acid sequences of β‐subunit tryptic peptides labeled with 2‐azido‐ATP (1987)

Under appropriate conditions tight, noncovalent binding of 2-azido-adenine nucleotides to either catalytic or noncatalytic binding sites on the E. coli F1-ATPase occurs. After removal of unbound ligands, UV-irradiation results primarily in the covalent incorporation of nucleotide moieties into the beta-subunit in both catalytic and noncatalytic site labeling experiments. Minor labeling of the alpha-subunit was also observed. After trypsin digestion and purification of the labeled peptides, microsequencing studies identified two adjacent beta-subunit tryptic peptides labeled by 2-azido-ADP or -ATP. These beta-subunit peptides were labeled on tyrosine-331 (catalytic sites) and tyrosine-354 (noncatalytic sites) in homology with the labeling patterns of the mitochondrial and chloroplast enzymes.
Characteristics of New Nickel Porous Alloy for Cathode Current Collector in Solid Oxide Fuel Cells (2017)

Ni–Sn porous alloys with different concentrations of Sn were prepared as potential current collectors in solid oxide fuel cells (SOFCs). The weight increase of these species was evaluated after heat-treatment under elevated temperatures in air for thousands of hours to evaluate the long-term oxidation resistance. For the porous metal with 10 wt% of Sn, the electrical resistivity at 600°C was almost constant at approximately 0.02 Ω cm2 up to an elapsed time of 1000 h. In addition, the gas diffusibility and the current-collecting ability of the porous metal were equivalent to those of a platinum mesh when applied in the cathode current collector of a SOFC operated at 600°C. Ni–Sn porous alloys with adequate contents of Sn are believed to be promising cathode current collector materials in SOFCs for operation at temperatures below 600°C.
ALKYL-SUBSTITUTED BENZENES OBSERVED IN A SUPERSONIC EXPANSION (1979)

$^{1}$J. H. Callomon, T. M. Dunn, and I. M. Mills, Philos. Trans. R. Soc, (Lond,) A 259, 499 (1966): A. E. W. Knight, C. S. Parmenter, and M. W. Schuyler, J. Amer. Chem. Soc. 97, 2005 (1975); L. Wunsch, F. Metz, H.J. Neusser, and E. W. Schlag, J. Chem. Phys. 66, 386(1977); S. M. Beck, M. G. Liverman, D. L. Monts, and R. E. Smalley, J. Chem. Phys. 70, 232 (1979); and references therein.
Design and Assessment of an Interoperable Wireless Power Transfer System Using an Impedance-Based Method (2022)

With the recently published standard, wireless charging systems for electric vehicles (EVs) are at the stage of preparing for mass market production. In order to have success, safe, and efficient wireless power transfer (WPT) public charging needs to be ensured and therefore interoperability is a key element in the design of such systems. In this article, a design methodology based on impedance planes is presented, which enables consideration of many interoperability aspects at an early stage in the design and has a high degree of flexibility. Four impedance interfaces are presented and operational boundaries and system requirements are added at each interface to establish capability ranges, which can be transferred to any other interface and used to define and assess the design space. Furthermore, this article shows how magnetic field leakage limitations can be considered in the design process. Experimental verification of the simulations is then undertaken and an example presented of how the primary electronics for a public charging use case scenario could be assessed or designed.
On the behaviour of a compact antenna system with non-resonant elements in the presence of the human head (2015)

A novel handset antenna technique to solve the increasing demand of mobile bands, the loading effects (mismatching and efficiency losses) and the power absorption introduced by the head is analysed in terms of bandwidth, efficiency and SAR (specific absorption rate). The technique proposed integrates non-resonant elements and its results are compared with those obtained by a planar inverted-F antenna. The main antenna parameters (bandwidth, efficiency in free-space, efficiency regarding the human head presence and SAR) are compared in terms of electromagnetic simulation and measurements. The study concludes that the novel antenna architecture achieves multiband operation from 824–960 MHz and 1710–2170 MHz and become robust to human loading while occupying a reduced volume of just 250 mm3 in a typical handset phone.
Electrochemical polymerization of PEDOT on catalyst-free patterned GaAs nanopillars for high efficiency hybrid photovoltaics 37<sup>th</sup> IEEE photovoltaic specialists conference (2011)

In this work, we present hybrid nanostructured core-shell solar cells based on patterned GaAs nanopillars grown by MOCVD, coated with electropolymerized poly(3,4-ethylenedioxythiophene) (PEDOT) polymer. The patterns are photolithographically defined and center-to-center pitch, hole size and mask arrangement can be precisely predetermined at nanometric resolution. Our inherently catalyst-free growth mode eliminates any metal (i.e. Au) diffusion into the nanopillars that could hinder the electron-hole pair extraction, paramount in photovoltaics. Hybrid devices [1-3] are normally obtained by spin-coating conjugated polymers on top of inorganic semiconducting surfaces. Spin-coating techniques allow to achieve only non-conformal thin films on planar substrates, yet it offers scarce controllability for 3-D structures where the polymer needs to be coated uniformly along the sides of the nanostructures too. Electrolytic deposition approaches, on the other hand, offer a simple, versatile route to high quality, conformal coatings to conductive surfaces regardless of the device structure. In addition, it possesses exquisite selectivity over the deposition position and precise control of the coating thickness and morphology, representing an attractive technique for fabricating core-shell inorganic-organic structured devices. This study demonstrates the rational design of a hybrid photovoltaic device through the careful tuning of the thickness, morphology, and physical/chemical properties of the polymer layer by electrodeposition, in conjunction with the manipulation over the size and properties of the patterned GaAs nanopillar arrays via MOCVD, in order to achieve optimized device configuration and performance. Device characterization is carried out in terms of photocurrent density-voltage (J-V) characteristics (under dark and standard AM 1.5 conditions), external quantum efficiency (EQE), and transient open-circuit voltage decay (TOCVD), standard figures of merit in the photovoltaic field.
The Critical Aspects of Data Analysis for Quantification in LIBS (2016)

International audience
Comparing a Novel PET Agent, Yttrium-86 Oxine, with Biopsy and FACS Analysis for Tracking in Vivo Migration Patterns of Different Preparations of DC from a Single Donor (2008)

Abstract Aim: The objective of this study was compare the in vivo distribution of different preparations of dendritic cells (DC) from a single donor after intradermal (ID) administration and correlate FACS analysis of excised tissue and imaging findings. Method: DC were generated from a single healthy donor apheresis product. The DC types were GM-CSF + IL-13 (IL-13 imDC), then further matured with FMKp+IFNg (IL-13mDC), GM-CSF + IL-4 (IL-4 imDC) and matured (IL-4 mDC) and GM-CSF+IFN-α (IFN-α DC). Antigen naive DC preparations were labelled with Yttrium-86 oxine (prepared on site) by incubation at room temperature for 30 minutes followed by centrifugation and resuspension. 5 × 105 radio-labelled cells in 50_L was injected ID into the footpad and the volar aspect of the foreleg of Balb/c nude mice (n=5 per group). A cohort (n=5) of control mice were similarly injected with Yttrium-86 oxine only. Images were acquired on a small animal PET (SAPET) (Mosaic, Philips Medical Systems) at 2, 24 and 45 hours post administration. Mice were then euthanized and popliteal, axillary and inguinal lymph nodes (LN) harvested. LN were processed into single cell suspension, stained with anti-human HLA-DR and CD11c and analysed by flow cytometry. SAPET images were graded: 0 – no signal, 1 – marginal, 2 – definite nodal signal, 3 – strong signal. DC detection by FACS was graded: 0 – no detection, 1 – &amp;lt;0.5% DC, 2 – 0.5.⋄1.5% DC, 3 – &amp;gt;1.5% DC of all viable cellular events. Results: Pre-injection analysis of DC showed expression of HLA-DR, HLA-ABC, CD11c, CD80 and CD86, low levels of CD83, CCR5 and CCR7 along with down regulation of CD14. Matured DC populations showed increased levels of CD83 and CD86. Similar phenotypic expression on the DC populations were observed prior to cryopreservation and upon thawing. Mean DC radiolabelling efficiency of 72% (54% – 88%). There was a minor decrease in cell numbers and viability after labelling most likely due to oxine related toxicity. There were no substantial differences in tracking between cell types. In 28 of 43 nodes assessed, FACS and SAPET results were congruent (27 FACS and SAPET positive and 1 FACS and SAPET negative). Of the 15 discrepant samples, 13 were SAPET positive and FACS negative, while 2 were FACS positive and PET negative. Average FACS and SAPET grades at 45 hours were higher for popliteal nodes than axillary nodes. No false positive nodal accumulation was visualised at 45 hours in control mice. Conclusion: This novel PET technique provides a robust, reproducible method for quantification of in vivo functional DC tracking to LN. Detection of DC migration using this novel PET agent is a more reliable technique than biopsy and FACS analysis, which has an inherent sampling error.
Treatment Outcomes of Patients with HIV and Tuberculosis (2007)

HIV-infected patients who received a 6-month rifamycin-based course of tuberculosis treatment or who received intermittent therapy had a higher relapse rate than HIV-infected subjects who received longer therapy or daily therapy, respectively. Standard 6-month therapy may be insufficient to prevent relapse in patients with HIV.
Monoclonal Antibody Therapy for Genitourinary Oncology: Promise for the Future (2002)

The field of mAb based immunotherapy continues to evolve. New discoveries in this burgeoning area of cancer therapy show promise for the future.
Modeling Stray Capacitances of High-Voltage Capacitive Dividers for Conventional Measurement Setups (2021)

Stray capacitances (SCs) are a serious issue in high-voltage (HV) applications. Their presence can alter the circuit or the operation of a device, resulting in wrong or even disastrous consequences. To this purpose, in this work, we describe the modeling of SCs in HV capacitive dividers. Such modeling does not rely on finite element analysis or complicated geometries; instead, it starts from an equivalent circuit of a conventional measurement setup described by the standard IEC 61869-11. Once the equivalent model including the SCs is found, closed expressions of the SCs are derived starting from the ratio error definition. Afterwards, they are validated in a simulation environment by implementing various circuit configurations. The results demonstrate the expressions applicability and effectiveness; hence, thanks to their simplicity, they can be implemented by system operators, researchers, and manufacturers avoiding the use of complicated methods and technologies.
Temperature issues in saliency-tracking based sensorless methods for PM synchronous machines (2010)

High frequency carrier signal excitation has been widely investigated for the position sensorless control of permanent magnet synchronous machines (PMSM's) near and at zero speed. Selection of the injected signal characteristics (shape, frequency, magnitude, etc.) is a trade-off between performance criteria of the sensorless control (stability, accuracy, robustness, bandwidth, etc.) and minimization of its adverse effects (additional losses, noise, vibration, etc.). The increased losses due to the injected signal can be of importance in PMSM's, since they can produce a significant increase of the temperature in the rotor magnets. This can adversely impact the normally operation of the machine and can eventually result in the irreversible demagnetization of the magnets. This paper analyzes the impact that excitation using a high frequency signals for sensorless control of PMSM's has on the machine's temperature. The machine design, as well as the type of injected high frequency signal, will be shown to strongly influence the machine's thermal behavior. Analytical models will be developed to explain this behavior, with experimental results being used to verify the analysis.
Color processing in camera phones: How good does it need to be? (2005)

As the fastest-growing consumer electronics device in history, the camera phone has evolved from a toy into a real camera that competes with the compact digital camera in image quality. Due to severe constraints in cost and size, one key question that remains unanswered for camera phones is: how good does the image quality need to be so that resource can be allocated most efficiently. In this paper, we have tried to find the color processing tolerance through a study of 24 digital cameras from six manufacturers under five different light sources. We measured both the inter-brand (across manufacturers) and intra-brand (within manufacturers) mean and standard deviation for white balance and color reproduction. The white balance results showed that most cameras didn’t follow the complete white balance model. The difference between the captured white patch and the display white point increased when the correlated color temperature (CCT) of the illuminant was further away from 6500K. The standard deviation of the red/green and blue/green ratios for the white patch also increased when the illuminant was further away from 6500K. The color reproduction results revealed a similar trend for the inter-brand and intra-brand chromatic difference of the color patches. The average inter-brand chromatic difference increased from 3.87 &Delta;E units for the &Delta;65 light (6500K) to 10.13 &Delta;E units for the Horizon light (2300K).
Contributors to the Lab on a Chip Cell and Tissue Engineering in Microsystems special issue (2007)

10.1039/b707012h
Distribution of the Mr 52,000 estrogen-regulated protein in benign breast diseases and other tissues by immunohistochemistry. (1986)

A secreted glycoprotein with a molecular weight of 52,000 is induced by estrogen in breast cancer cells and has been purified to prepare monoclonal antibodies. The protein has been detected in some breast cancers but not in normal breast and uterus. In order to study its potential value as a marker, we have tested by immunohistochemistry frozen sections of several normal and malignant tissues and of benign mastopathies. Among different tissues tested, the Mr 52,000 protein was detected only in liver, sweat glands, and some sebaceous glands, and in malignant melanomas and some breast tumors. Other estrogen-responsive tissues (ovary, placenta, endometrium, etc.) gave negative results. Immunoradiometric assay of the Mr 52,000 protein in biological fluid revealed an elevated concentration in cyst fluid (0.5 to 7.4 micrograms/ml), pleural effusions of certain metastatic breast cancer, and sweat. By immunohistochemistry, the Mr 52,000 antigen was also detected in 42% of 129 benign mastopathies. Gynecomastia, fibrous disease, fibroadenoma, and adenosis were mainly negative, whereas ductal hyperplasia and cysts were positive. The Mr 52,000 protein was found mostly in proliferative ducts and in cysts but not in lobular hyperplasia and nonproliferative lesions without cyst. More Mr 52,000 protein was found in postmenopausal patients than in premenopausal patients. We conclude that the Mr 52,000 protein is a marker associated with mammary cysts and proliferative ducts. On the basis of the increased risk of breast cancer in proliferative mastopathies, we suggest that the Mr 52,000 protein is useful for predicting high-risk mastopathies acting as a marker associated with the proliferation of ductal tissue.
The Third International Consensus Guidelines on the Management of Cytomegalovirus in Solid-organ Transplantation (2018)

Despite recent advances, cytomegalovirus infections remain one of the most common complications affecting solid organ transplant recipients, conveying higher risks of complications, graft loss, morbidity, and mortality. Research in the field and development of prior consensus guidelines supported by The Transplantation Society has allowed a more standardized approach to cytomegalovirus management. An international multidisciplinary panel of experts was convened to expand and revise evidence and expert opinion-based consensus guidelines on CMV management including prevention, treatment, diagnostics, immunology, drug resistance, and pediatric issues. Highlights include advances in molecular and immunologic diagnostics, improved understanding of diagnostic thresholds, optimized methods of prevention, advances in the use of novel antiviral therapies and certain immunosuppressive agents, and more savvy approaches to treatment resistant/refractory disease. The following report summarizes the updated recommendations.
A Non-Hierarchical Cell Search Scheme (2007)

Cell search is the procedure by which a mobile terminal acquires time and frequency synchronization to the network and detects a cell identity. In this paper, we give a non-hierarchical scheme for OFDM systems, where cell-specific signals are concurrently used for synchronization and cell identification. By using time-domain symmetric signals, the symbol timing is accurately found by a reverse differential correlator. Once the timing is established and frequency offsets are estimated, detection of the transmitted synchronization sequence is done non-coherently by means of a differential decoder. The frame timing is obtained by letting different synchronization signals represent elements of cyclically unique codewords over a radio frame. The scheme can convey large amount of cell identities and numerical results show that it is robust to frequency offsets.
Superoxide Inhibits Guanine Nucleotide Exchange Factor (GEF) Action on Ras, but not on Rho, through Desensitization of Ras to GEF (2014)

Ras and Rho GTPases are molecular switches for various vital cellular signaling pathways. Overactivation of these GTPases often causes development of cancer. Guanine nucleotide exchange factors (GEFs) and oxidants function to upregulate these GTPases through facilitation of guanine nucleotide exchange (GNE) of these GTPases. However, the effect of oxidants on GEF functions, or vice versa, has not been known. We show that, via targeting Ras Cys(51), an oxidant inhibits the catalytic action of Cdc25-the catalytic domain of RasGEFs-on Ras. However, the enhancement of Ras GNE by an oxidant continues regardless of the presence of Cdc25. Limiting RasGEF action by an oxidant may function to prevent the pathophysiological overactivation of Ras in the presence of both RasGEFs and oxidants. The continuous exposure of Ras to nitric oxide and its derivatives can form S-nitrosated Ras (Ras-SNO). This study also shows that an oxidant not only inhibits the catalytic action of Cdc25 on Ras-SNO but also fails to enhance Ras-SNO GNE. This lack of enhancement then populates the biologically inactive Ras-SNO in cells, which may function to prevent the continued redox signaling of the Ras pathophysiological response. Finally, this study also demonstrates that, unlike the case with RasGEFs, an oxidant does not inhibit the catalytic action of RhoGEF-Vav or Dbs-on Rho GTPases such as Rac1, RhoA, RhoC, and Cdc42. This result explains the results of the previous study in which, despite the presence of an oxidant, the catalytic action of Dbs in cells continued to enhance RhoC GNE.
Opportunistic access in slotted aloha adapted to decentralized CSI (2005)

We develop a model for decentralized opportunistic transmissions of a finite (n) user slotted ALOHA system that relies on decentralized channel state information (D-CSI). D-CSI refers to each user node having access only to its own uplink channel gain. Every user adapts both rate and re-transmission probability to D-CSI in every slot. Due to the variable transmission rate from slot to slot, incoming packets with fixed length are re-assembled to deliver as many bits as possible per slot. By modeling the number of bits in queues as a discrete Markov chain, we analyze the stability region and the maximum stable throughput (MST). In addition, we derive a decentralized opportunistic scheme and show that it achieves a fraction (1-1/n)/sup n-1/ of its centralized counterpart's throughput, where (1-1/n)/sup n-1/ is a factor due to the inherent contention in random access.
Two‐year extractability of novel left ventricular, active fixation leads in the sheep model (2017)

Extraction of the side helix, active fixation LV lead from the coronary veins in the sheep model is safe, without procedural complexity, and free of complications after long-term LV lead implant duration.
Computational design and experimental evaluation of glycosyltransferase mutants: engineering of a blood type B galactosyltransferase with enhanced glucosyltransferase activity (2006)

Glycosyltransferases are an enormous and diverse class of enzyme encompassing 1% of all sequenced genomes. They catalyze the transfer of a monosaccharide from an activated donor such as a sugar-nucleotide to an acceptor molecule. Though the primary sequences of glycosyltransferases have little homology, X-ray structural studies on glycosyltransferases have revealed that there are two main folds and that the orientation of the sugar donors with respect to the folds is highly conserved. It seems that glycosyltransferases have evolved diversified specificities toward donor sugars by changing the amino acids around the monosaccharide moiety without altering the orientation of the nucleotide moiety. In this study, we designed new glycosyltransferases with altered donor specificities by use of a novel empirical model called the Epimer Propensity Index (EPI). The EPI was constructed using 221 carbohydrate-protein complex structures in the Protein Data Bank with either galactose or glucose in the complex. The blood type B synthesizing glycosyltransferase GTB, a galactosyltransferase was our target enzyme. Two GTB mutants designed to exhibit enhanced glucosyltransferase activity were cloned, expressed and characterized experimentally. The predicted GTB mutants, Ser185Asn and Ser185Cys, exhibited 4.3- and 4.8-fold elevations in k(cat)/K(m) for UDP-Glc relative to that of wild-type enzyme.
Design and construction of a telescope simulator for LISA optical bench testing (2017)

LISA (Laser Interferometer Space Antenna) is a proposed space-based instrument for astrophysical observations via the measurement of gravitational waves at mHz frequencies. The triangular constellation of the three LISA satellites will allow interferometric measurement of the changes in distance along the arms. On board each LISA satellite there will be two optical benches, one for each testmass, that measure the distance to the local test mass and to the remote optical bench on the distant satellite. For technology development, an Optical Bench Elegant Bread Board (OB EBB) is currently under construction. To verify the performance of the EBB, another optical bench - the so-called telescope simulator bench - will be constructed to simulate the beam coming from the far spacecraft. The optical beam from the telescope simulator will be superimposed with the light on the LISA OB, in order to simulate the link between two LISA satellites. Similarly in reverse, the optical beam from the LISA OB will be picked up and measured on the telescope simulator bench. Furthermore, the telescope simulator houses a test mass simulator. A gold coated mirror which can be manipulated by an actuator simulates the test mass movements. This paper presents the layout and design of the bench for the telescope simulator and test mass simulator.