gebhart/patrepeval huggingface dataset:
572 Gut microbiota tuning promotes tumor-associated antigen cross-presentation and enhances CAR T antitumor effects (2022)

<h3>Background</h3> CAR T cell therapy has shown impressive clinical responses in B cell lymphoma and leukemia, and to date, four CAR T therapies targeting CD19 have been approved by the Food and Drug Administration for the treatment of CD19-positive hematologic malignancies. Notwithstanding the encouraging results, a significant proportion of patients experience a lack of response or disease relapse. Recently several studies have shown the influence of commensal microbes on T cell function, specifically in the setting of checkpoint immunotherapy. We previously reported that gut microbiota perturbation by oral vancomycin, affects dendritic cell antigen presentation and cytokine secretion profile and affects the outcome of radiation and CAR-T therapies. Based on these data, in the present study we explore how modulation of the gut microbiota by vancomycin would influence the outcome of CART-19 therapy. <h3>Methods</h3> We employed two murine tumor models, the hematopoietic CD19+ A20 lymphoma and the CD19+ B16 melanoma. We injected BALB/c or C57/black6 mice subcutaneously with 2x10<sup>6</sup> A20 or 1x10<sup>5</sup> CD19 positive B16 tumor cells. The same day we started treatment with oral vancomycin. Nineteen days after A20 inoculation or eleven days after CD19+ B16, mice were lymphodepletion with total body radiation (TBI). Two days later, CART-19 cells containing second generation fully murine CAR with a 4-1BB costimulatory domain were infused by tail vein. To validate the data in humans, we proceeded by: a. generating “human gut microbiota avatars”, antibiotics preconditioned recipient mice were engrafted with human gut microbiota and enrolled in similar experiments mentioned before, and b. evaluating CART-19 peak expansion on lymphoblastic leukemia patients receiving vancomycin. <h3>Results</h3> In both models, mice receiving vancomycin in combination with CD19 directed CAR T cell therapy displayed increased tumor-associated antigens (TAAs) presentation and tumor control compared to CART-19 alone. Fecal microbiota transplant from human healthy donors to pre-conditioned mice recapitulated the results obtained in naïve gut microbiota mice. Lastly, B-cell acute lymphoblastic leukemia patients treated with CART-19 and exposed to oral vancomycin showed higher CART-19 peak expansion compared with matched unexposed patients. <h3>Conclusions</h3> These results highlight the role of the gut microbiota on immunotherapy and suggest that the modulation of the gut microbiota using vancomycin affects the outcome of CAR T cell therapy. <h3>Trial Registration</h3> NCT02030847 <h3>Ethics Approval</h3> This study was approved by the Internal Review Board at the Hospital of the University of Pennsylvania.
Matrix-assisted laser desorption/ionization-tandem mass spectrometry with high resolution and sensitivity for identification and characterization of proteins (2002)

Although peptide mass fingerprinting is currently the method of choice to identify proteins, the number of proteins available in databases is increasing constantly, and hence, the advantage of having sequence data on a selected peptide, in order to increase the effectiveness of database searching, is more crucial. Until recently, the ability to identify proteins based on the peptide sequence was essentially limited to the use of electrospray ionization tandem mass spectrometry (MS) methods. The recent development of new instruments with matrix-assisted laser desorption/ionization (MALDI) sources and true tandem mass spectrometry (MS/MS) capabilities creates the capacity to obtain high quality tandem mass spectra of peptides. In this work, using the new high resolution tandem time of flight MALDI-(TOF/TOF) mass spectrometer from Applied Biosystems, examples of successful identification and characterization of bovine heart proteins (SWISS-PROT entries: P02192, Q9XSC6, P13620) separated by two-dimensional electrophoresis and blotted onto polyvinylidene difluoride membrane are described. Tryptic protein digests were analyzed by MALDI-TOF to identify peptide masses afterward used for MS/MS. Subsequent high energy MALDI-TOF/TOF collision-induced dissociation spectra were recorded on selected ions. All data, both MS and MS/MS, were recorded on the same instrument. Tandem mass spectra were submitted to database searching using MS-Tag or were manually de novo sequenced. An interesting modification of a tryptophan residue, a "double oxidation", came to light during these analyses.
Atmospheric Turbulence Degraded-Image Restoration by Kurtosis Minimization (2009)

Atmospheric turbulence is caused by the random fluctuations of the refraction index of the medium. It can lead to blurring in images acquired from a long distance away. Since the degradation is often not completely known, the problem is viewed as blind image deconvolution or blur identification. Our previous work has observed that blurring increases kurtosis and introduced a new blur identification method based on kurtosis minimization (KM). In this letter, this observation has been studied using phase correlation. The KM method is compared with two other signal processing methods. The limitation of the method is also discussed.
Overcoming the Challenge of Security in a Mobile Environment (2006)

The secure operation of ad hoc networks faces the novel challenge of location verification on top of the security challenges that wireline networks face. The novelty lies in the fact that a node can correctly validate who it is, but lie about its location and exploit this to create problems to the network. There are three main factors that make ad hoc networks more vulnerable: (a) nodes can overhear other nodes announcements, (b) nodes can lie about their location, and (c) nodes can avoid detection and isolation by moving. As a result, malicious nodes can fake their position and this way obstruct the routing. In this work, we explain how location and topology related malice can affect the security of wireless ad hoc networks. First, we present the most important attacks that can stem from misuse of location information. Second, we provide an overview of security routing approaches. Although several of the current techniques are promising, we conclude that there does not exist a bulletproof approach as of yet
A DFT and HF quantum chemical study of the tin nanocluster [(RSn)12O14(OH)6]2+ and its interactions with anions and neutral nucleophiles: confrontation with experimental data (2002)

Calculations of traditional wave function and DFT based reactivity descriptors are reported for the nanocluster [(RSn)12O14(OH)6]2+ (R = CH3) in order to get insight into the factors determining the exact nature of its interactions with anions or neutral nucleophiles (F−, Cl−, OH−, H2O, acetone, DMSO). Two levels of calculation (Hartree–Fock and DFT) were used in this study, giving the opportunity to compare the performance of both aproaches. As a whole the HF and DFT results, despite some numerical differences, are qualitatively and in most cases quantitatively similar, since no interchanges in the sequences are found. Calculated charge distributions indicate that the hexacoordinated tin atoms are expected to be harder, the pentacoordinated ones softer. This result is confirmed by the condensed local softness and experimental observations provided by the literature (X-ray, 119Sn NMR), thereby matching predictions made on the basis of the HSAB principle. Molecular electrostatic potential (MEP) calculations further confirm this selectivity, indicating that nucleophiles will approach preferentially the macrocation around the poles rather than at the equator of the cage. Calculated stabilization energies indicate that the charged (F−, Cl− and OH−) and uncharged (DMSO, acetone and water) nucleophiles tested all interact preferentially with the cage pole region around the hexacoordinated tin atoms. This feature appears related to the electrostatic nature of the interaction for the charged nucleophiles and to the tendency, for all the nucleophiles, to form hydrogen bridges with the μ2-OH moieties. The basicity of the three types of oxygen atoms [μ2-OH, μ3-O(I), μ3-O(P)] present in [(RSn)12O14(OH)6)]2+ has been addressed by protonation energy and MEP calculations, taking into account accessibility factors not represented by atomic charges. Summarizing, it turns out that the hardness/softness properties of tin atoms are modulated by their coordination; however, the sole consideration of these quantities is not sufficient to predict in all cases the strength and orientation of the interaction between various nucleophiles and the cluster, since electrostatic interactions and H-bonding play a major role.
Transforming Toxic Leaders (2009)

This book argues that toxic leadership presents organizations with opportunities for positive transformation, innovation, growth, and development. The author offers a unique 'Model of Leader Detoxification' as a central blueprint for successfully managing toxic leaders and growing healthier, visionary companies.
A Reinforcement Learning Framework for Parameter Optimization in Elastic Optical Networks (2020)

We present a reinforcement learning (RL) framework for maximizing the total capacity of a 51-channel transmission system, which runs magnitudes faster than a genetic algorithm (GA) based optimization. The generalization capabilities and performance of the RL framework are compared to results obtained with a GA.
Summary Statement: Safety of Bone Morphogenetic Proteins for Spine Fusion (2002)

As any new technology approaches widespread clinical use, the safety profile must be of paramount concern. This article reviews the available safety data for the various bone morphogenetic protein programs. On the basis of available information, each BMP requires its own safety profile of local, regional, and systemic side effects. When used properly, BMPs currently appear to be extremely safe for spine fusion.
Loss Analysis in Perovskite Photovoltaic Modules (2019)

Hybrid metal‐halide perovskite‐based thin‐film photovoltaics (PVs) have the potential to become the next generation of commercialized PV technology with certified power conversion efficiencies reaching 24% on devices having 0.1 cm 2 area. Recent efforts in upscaling this technology result in an efficiency of 12.6% for 354 cm 2 modules. However, upscaling loss for perovskite‐based PVs is higher than for any other PV technology. In this study, upscaling losses of devices with aperture area 0.1, 4, and 100 cm 2 are investigated, with a focus on layer inhomogeneities. Electroluminescence, dark lock‐in thermography, microphotoluminescence spectroscopy, and electron spectroscopy are used to analyze and group layer inhomogeneities with a minimal size of 10 μm and to compare loss mechanisms for radial and linear deposition techniques. Analysis results help to identify current processing pitfalls, where understanding and control of perovskite crystal formation plays the crucial role.
Optical frequency sweep technique using single sideband modulation (2005)

We demonstrated optical frequency sweep using optical single sideband modulation technique, where the frequency shift range was 2 GHz-10 GHz. Undesired sidebands due to electric phase errors can be compensated by synchronized bias control. (2 pages)
Photo-stabilised microemulsions (2005)

Light-induced stabilisation of water-in-heptane microemulsions has been achieved with a UV-sensitive gemini photo-surfactant.
Building problem solvers: listings (1993)

This supplement to Building Problem Solvers contains the Common Lisp code examples referenced throughout the text. The code is available on disk and can also be downloaded via ftp.
A Framework of a Model-Based Simulation System for Prediction of Surface Generation in Fast Tool Servo Machining of Optical Microstructures (2007)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.
26GHz ray-tracing pathloss prediction in outdoor scenario in presence of vegetation (2018)

In this paper 26GHz propagation in outdoor urban environment is investigated using path-loss measurements and ray-tracing simulation. The impact of advanced implementations such as diffuse scattering and vegetation attenuation is verified versus measurements in an outdoor scenario. The results show that the contribution from non-specular components in nonline-of-sight conditions is much more important than expected to achieve a good prediction accuracy at this frequency band. Moreover, vegetation attenuation is also very relevant and can be modeled using a simple, specific attenuation formula. Despite some deficiencies in the input database, the prediction accuracy is good and simulations are generally able to track the measurements behavior.
Investigations on Optimum Reference Signal Structure for Single-Carrier FDMA Radio Access in Evolved UTRA Uplink (2007)

This paper presents the optimum reference signal (RS) structure for single-carrier (SC)-FDMA radio access using discrete Fourier transform (DFT)-spread OFDM to satisfy the requirements in the Evolved UTRA uplink. The simulation results shows that the optimum fast Fourier transform (FFT) block length, i.e., data block length, is approximately 66.7 – 133.3 μsec, corresponding to the FFT length of 1024 – 2048 points assuming the sampling frequency of 15.36 Mega samples per second. Moreover, it is shown that an RS structure that comprises two RS blocks contained within the duration of a 1-msec sub-frame is the most promising candidate based on superior user throughput under low mobility conditions such as less than approximately 120 km/h and on a high degree of commonality of radio parameters such as that in the downlink, considering the fairly low applicability of 16QAM modulation under extremely high mobility conditions such as 350km/h.
Enterprise Knowledge Agents: Agent-Based Discovery of Like-Minded Individuals (2008)

Agent-based computing environments provide the capability to establish an enterprise of autonomous agents that collaborate and share information on common interests. Discovery of new data can alert users to changes in the information environment of importance to them. The goal of this research is to define an agent environment that models an organization's tacit knowledge. This document proposes a process to find named entities in the content of documents and to derive context from a user community. The EKA system ingests RSS feeds of data sources that are representative of separate users, extracts entities from the text, and provides context on each entity using related tags. Knowledge representations of the news feeds are created, managed, and reasoned over by a collection of agents. Agents will respond to pre-registered topics and provide notifications when those topics have been identified. The purpose here is to illustrate that an agent can serve as a broker of knowledge and assist users in identifying like-minded individuals through an analysis of the data the user is working with and initiate collaboration.
Mathematical Modeling of an Internal-Reforming, Carbonate Fuel Cell Stack (2004)

The objective of this work is the development of a practical computational model of a carbonate fuel cell stack. Previously published carbonate fuel cell models have focused more on the fundamental mechanisms of fuel cell operation than on evaluation of practical fuel cell product designs. Efficient development of a fuel cell product requires a predictive tool that couples all the important mechanisms with the capability to evaluate the performance of many design iterations quickly. The important mechanisms typically include three dimensional fluid flow, heat and mass transfer, gas-phase and surface chemistry, electrochemistry and structural mechanics. For large-scale fuel cell with applications in the power generation industry, thermal management is of significant interest for stack performance, reliability and life. Minimizing peak cell temperatures improves cell life and minimizing stack temperature gradients reduces stack thermal stress and improves cell performance. In this paper, the fuel cell model is presented along with experimental data validating its accuracy and predictive capabilities. The tool has proven to be a valuable asset for design evaluation and optimization of fuel cell stack designs at FuelCell Energy.
A Randomized Phase 2 Trial Comparing Omidenepag Isopropyl 0.002% Once and Twice Daily in Subjects With Primary Open-angle Glaucoma or Ocular Hypertension (SPECTRUM-6) (2021)

In this study, the benefit-risk profile of omidenepag isopropyl 0.002% QD was more favorable than the benefit-risk profile of BID. This difference was driven by a higher incidence of local tolerability issues in the BID arm.
Tristetraprolin regulation of interleukin 23 mRNA stability prevents a spontaneous inflammatory disease (2013)

Interleukin (IL) 12 and IL23 are two related heterodimeric cytokines produced by antigen-presenting cells. The balance between these two cytokines plays a crucial role in the control of Th1/Th17 responses and autoimmune inflammation. Most studies focused on their transcriptional regulation. Herein, we explored the role of the adenine and uridine-rich element (ARE)-binding protein tristetraprolin (TTP) in influencing mRNA stability of IL12p35, IL12/23p40, and IL23p19 subunits. LPS-stimulated bone marrow-derived dendritic cells (BMDCs) from TTP(-/-) mice produced normal levels of IL12/23p40. Production of IL12p70 was modestly increased in these conditions. In contrast, we observed a strong impact of TTP on IL23 production and IL23p19 mRNA stability through several AREs in the 3' untranslated region. TTP(-/-) mice spontaneously develop an inflammatory syndrome characterized by cachexia, myeloid hyperplasia, dermatitis, and erosive arthritis. We observed IL23p19 expression within skin lesions associated with exacerbated IL17A and IL22 production by infiltrating γδ T cells and draining lymph node CD4 T cells. We demonstrate that the clinical and immunological parameters associated with TTP deficiency were completely dependent on the IL23-IL17A axis. We conclude that tight control of IL23 mRNA stability by TTP is critical to avoid severe inflammation.
Large Domain Film of Bis(styrilanthracene) Derivatives Prepared by a Simple Melt-Freezing Process and the Improvement of Field Effect Hole Mobility (2008)

Bis(styrylanthracene) (BSA) derivatives with long alkyl side chains form thin films consisting of large domains (mm-scale) at room temperature by a simple melt-freezing process. It is revealed by X-ray diffraction that the homogeneous domains are in a single-crystal-like solid state. The film assumes a layered structure with π–π stacking parallel to the film plane. The hole mobility of the field effect transistor composed of the monodomain film is almost two orders of magnitude higher than that of the corresponding amorphous film. This result may be due to π–π stacking developed in the carrier transport direction.
+Three-step H<inf>2</inf>Se/Ar/H<inf>2</inf>S reaction of metal precursors for large area Cu(In,Ga)(Se,S)<inf>2</inf> with uniform Ga distribution (2013)

A three-step H <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</inf> Se/Ar/H <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</inf> S reaction is used to process Cu-In-Ga metal precursors to form Cu(In,Ga)(Se,S) <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</inf> films over 10 × 10 cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> substrates. The 1 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">st</sup> selenization step gives fine microstructure with Ga accumulation near the Mo back contact, primarily in a Cu <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">9</inf> Ga <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">4</inf> phase. Significant grain growth with homogenous through-film Ga distribution is obtained by the 2 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">nd</sup> Ar annealing step. The 3 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">rd</sup> sulfization step completes the reaction process and incorporates S near the Cu(In,Ga)Se <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</inf> surface. The resulting films show good adhesion and yielded devices with η = 14.8% and V <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">OC</inf> = 612 mV.
Lithium-Assisted Copolymerization of CO2/Cyclohexene Oxide: A Novel and Straightforward Route to Polycarbonates and Related Block Copolymers (2016)

A facile route toward alternating polycarbonates by anionic copolymerization of carbon dioxide (CO2) and cyclohexene oxide (CHO), using lithium halide or alkoxide as initiators and triisobutylaluminum (TiBA) as activator, is reported. α,ω-Heterobifunctional and α,ω-dihydroxypoly(cyclohexene carbonate)s (PCHC) as well as poly(CHC-co-CHO) copolymers with different carbonate composition could also be easily synthesized by adjusting the amount of TiBA or by adding inert lithium salts. The value of this initiating system also resides in the easy access to PSt-b-PCHC (PSt: polystyrene) and PI-b-PCHC (PI: polyisoprene) block copolymers which can be derived by mere one-pot sequential addition of styrene or dienes first and then of CO2 and CHO under the same experimental conditions.
CW DUV light sources for inspection tools (2005)

We describe true continuous-wave (CW), high-power, line-narrowed, deep-ultraviolet (DUV) light sources for the high-resolution metrology tools such as wafer inspection and mask inspection systems. The 198.5-nm CW radiation with 300-mW power has also been achieved by sum-frequency mixing (SFM) of 1064-nm output from a single-frequency Yb<sup>3+</sup> fiber amplifier with the 244-nm radiation from a frequency-doubled argon-ion laser. The 266-nm CW DUV radiation with 5 W of maximum power has been generated by frequency doubling of 532-nm green laser output. Both sources utilize Brewster-cut CsLiB<sub>6</sub>O<sub>10</sub> (CLBO) crystal for efficient and stable DUV light generation.