1,25-Dihydroxycholecalciferol Enhances the Expression of MHC Class II Antigens and Intercellular Adhesion Molecule-1 by Human Renal Tubular Epithelial Cells (1996)

No AccessJournal of UrologyInvestigative Urology1 Apr 19961,25-Dihydroxycholecalciferol Enhances the Expression of MHC Class II Antigens and Intercellular Adhesion Molecule-1 by Human Renal Tubular Epithelial Cells B. Kreft, S. Brzoska, C. Doehn, M.R. Daha, F.J. Van Der Woude, and K. Sack B. KreftB. Kreft More articles by this author , S. BrzoskaS. Brzoska More articles by this author , C. DoehnC. Doehn More articles by this author , M.R. DahaM.R. Daha More articles by this author , F.J. Van Der WoudeF.J. Van Der Woude More articles by this author , and K. SackK. Sack More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)66305-5AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Beside its role in calcium and phosphorus metabolism, 1,25-dihydroxycholecalciferol (1,25-D3) exerts multiple effects on cytokine and major histocompatibility complex (MHC) class II expression in monocytes and lymphocytes. In different renal diseases tubular epithelial cells express MHC class II molecules and cell adhesion molecules, such as intercellular adhesion molecule-1 (ICAM-1). Therefore, modulation of MHC class II and ICAM-1 expression in renal tubular epithelial cells by 1,25-D3 may be relevant to lymphocyte adhesion to tubular epithelial cells and immune mediated renal injury. However, the expression of MHC class II antigens and cellular adhesion molecules by renal tubular epithelial cells in response to 1,25-D3 has not been investigated. Materials and Methods: We generated human renal tubular epithelial cells and SV40 transfected tubular epithelial cells to investigate immune modulation of 1,25-D3 on renal epithelial cells. Major histocompatibility complex class II molecules and ICAM-1 were detected by a specific enzyme linked immunoassay. Results: We found a dose-dependent increase of both constitutive and induced MHC class II and ICAM-1 expression in tubular epithelial cells stimulated with 1,25-D3. Dose-dependent stimulation of MHC class II and ICAM-1 expression was not restricted to primary human renal tubular epithelial cells but was also detected in SV40 transfected cells. Conclusions: Expression of MHC class II and ICAM-1 is crucial for antigen presentation by and lymphocyte adhesion to renal tubular epithelial cells. 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Kidney Int.1986; 29: 1209. Google Scholar 32 : In vivo effect of 1,25-dihydroxyvitamin D3 on phagocyte function in hemodialysis patients. Kidney Int.1991; 40: 927. Google Scholar Departments of Internal Medicine and Urology, Medical University of Lubeck, Lubeck, Germany and the Department of Nephrology, Academisch Ziekenhuis Leiden, Leiden, The Netherlands© 1996 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 155Issue 4April 1996Page: 1448-1453 Advertisement Copyright & Permissions© 1996 by American Urological Association, Inc.MetricsAuthor Information B. Kreft More articles by this author S. Brzoska More articles by this author C. Doehn More articles by this author M.R. Daha More articles by this author F.J. Van Der Woude More articles by this author K. Sack More articles by this author Expand All Advertisement PDF downloadLoading ...