gebhart/patrepeval huggingface dataset:
Rastreamento do uso de álcool entre motoristas, após a implementação da Lei Seca Tracking of alcohol use among drivers after implementation of Brazil's Drink-driving Law Rastreo del uso de alcohol entre conductores, tras la implementación de la Ley Seca (2015)

This cross-sectional study to identify patterns of drinking among motor vehicle drivers and to associate this with drink-driving behavior at a Traffic Department (DETRAN) station in Rio de Janeiro City. Data were collected in 2011 by ap- plying the Alcohol use disorders identification test (AUDIT) questionnaire, with additional drink-driving-related variables, to the 390 study participants, and tabulated using Epi-Info 2000 software. Analysis revealed a significant frequency of drivers classified by AUDIT as problematical drinkers, who reported drinking and driving, even after enactment of Law 11.705/08.
Frequency of Permanent Pacemaker or Implantable Cardioverter-Defibrillator Infection in Patients with Gram-Negative Bacteremia (2006)

Background. Despite the frequent occurrence of bacteremia due to gram-negative organisms in patients with underlying permanent pacemakers (PPMs) or implantable cardioverter defibrillators (ICDs), the outcome and treatment of these patients has received scant attention. In patients with PPMs or ICDs who have Staphylococcus aureus bacteremia, 45% have PPM/ICD infection. Methods. We conducted a retrospective cohort study over a 7-year period to assess the clinical features and frequency of PPM/ICD infection in patients with gram-negative bacteremia, as well as the incidence of relapse in patients for whom the device was not removed. Results. Forty-nine patients were included in the study; 3 (6%) had either definite (2 patients) or possible (1 patient) PPM/ICD infection. Both patients with definite PPM/ICD infection had clear infection of the generator pocket. None of the other patients with alternate sources of bacteremia developed PPM/ICD infection. Thirty-four patients with retained PPM/ICD were observed for >12 weeks (median time, 759 days), and 2 (6%) developed relapsing bacteremia, although they each had alternative sources of relapse. Conclusions. In sharp contrast to S. aureus infection, PPM/ICD infection in patients with gram-negative bacteremia was rare, and no patients appeared to have secondary PPM/ICD infection due to hematogenous seeding of the system. Despite infrequent system removal in these patients, relapsing bacteremia among patients who survived initial bacteremia was rarely seen. If secondary PPM/ICD infection occurs in patients with gram-negative bacteremia, it is either uncommon or it is cured with antimicrobial therapy despite device retention.
High Temperature Materials (2006)

The interdisciplinary nature of ECS is uniquely reflected within the High Temperature Materials (HTM) Division. Here, scientists and engineers are concerned with the chemical and physical characterization of materials, the kinetics of reactions, the thermodynamic properties and phase equilibria of systems, the development of new processing methods, and ultimately, the use of materials in advanced technology applications at high temperatures.
Network Methods for Elucidating the Complexity of Common Human Diseases (2013)

As big data comes to define the landscape in the life and biomedical sciences, carrying out successful research or providing the most appropriate diagnosis and treatment path for a given patient will more and more come to depend upon one’s ability to master the big data, to derive meaning and understanding from it. We can now score variations in DNA across whole genomes, RNA levels and alternative isoforms, metabolite levels, protein levels and protein state information, protein–protein interactions, and protein–DNA interactions, in a comprehensive fashion in entire populations of individuals. Interactions among these molecular entities define the complex web of biological processes that give rise to all higher order phenotypes, including disease. Most common forms of human disease such as schizophrenia, Alzheimer’s disease, and autism are not the result of single changes to single genes but rather emergent properties of the complex networks that define our systems at multiple scales (molecular, cellular, tissue, organ, organism, community). In order to elucidate the complexity of common human disease and other complex phenotypes, biological systems such as ourselves must be modeled as highly modular, fluid systems exhibiting a plasticity that allows them to adapt to a vast array of conditions. Leveraging the vast mountains of data to model biological systems in this way demands the development of analytical approaches that simultaneously integrate different dimensions of data. Here I motivate the need to take a more holistic approach to modeling biological systems and then detail different approaches that have been recently developed to carry out this type of modeling, from leveraging DNA variation as a systematic perturbation source to infer causal relationships among traits of interest, to the application of more advanced Bayesian network reconstruction algorithms to construct predictive network models of disease.
Figure S8 from Oral IRAK-4 Inhibitor CA-4948 Is Blood-Brain Barrier Penetrant and Has Single-Agent Activity against CNS Lymphoma and Melanoma Brain Metastases (2023)

&lt;p&gt;Supplemental Figure 8. (a) Representative IHC for Ki67 proliferative marker (pink pseudocolor) in B16F10 MBM tumors isolated on day 7 of treatment (day 12 of study) with vehicle control or CA-4948 (100 mg/kg). GFAP (astrocytes, green pseudocolor), CD45 (red pseudocolor), and DAPI nuclear stain (blue pseudocolor) included, scale bar = 100μm. (b) Quantitative comparison of Ki67 tissue expression shown in panel f between vehicle control (n=5) and CA-4948 (n=5), measured as the % of Ki67 positive cells within total cell population defined by DAPI nuclear count within the tumor area. P-value determined by unpaired t test.&lt;/p&gt;
Fungal Infections in Left Ventricular Assist Device Recipients (1995)

The authors reviewed the charts of 26 recipients of a left ventricular assist device to determine the incidence of fungal infections and the clinical course of these patients. Nine patients (35%) had positive fungal cultures. Of these, six had clinical infections and three were colonized asymptomatically. Three of the six infected patients (including one with mediastinal sepsis and another requiring left ventricular assist device replacement for intractable fungemia) underwent orthotopic heart transplantation after successful therapy. Of the remaining three, one died of a thromboembolic stroke (probably septic in nature), one died secondary to driveline rupture, and the third succumbed to culture-negative sepsis. Two of the colonized patients underwent transplantation, and the third succumbed to perioperative right sided circulatory failure and hypoxia. Positive fungal cultures were a common finding in our series. Because of a significant incidence of fungal infection-related morbidity, the authors revised their pre operative and post operative protocol to include: 1) 2 weeks of fluconazole therapy (200 mg intravenously daily) for patients receiving broad spectrum antibiotics and for those with evidence of preoperative fungal colonization; 2) daily dressing changes around drivelines; 3) daily nystatin swish and swallow; and 4) empiric fluconazole treatment for culture-negative sepsis. Using this protocol, three left ventricular assist device recipients received prophylactic fluconazole and had no evidence of fungal morbidity or mortality on short-term follow-up.
Integration of Wet Cleaning in 45 Nm Pitch BEOL Processing (2013)

A partial-trench via first with self-aligned double patterning (SADP) approach is used to realize a 45 nm pitch using a dual damascene compatible hardmask. This back-end of line (BEOL) integration approach is used to investigate the ability to integrate advanced low- k materials and more specifically to assess their patterning performance. Throughout this SADP integration approach, different specific cleaning steps are required. A first cleaning process is needed after spacer etch and amorphous-carbon (a-C) strip to remove the spacer etch residues that are formed. Further in the integration flow, a TiN clean is needed together with a post-etch residue removal (PERR) after low- k etch.
Translational Modulation of Proteins Expressed from Bicistronic Vectors (2009)

Bicistronic vectors are useful tools for exogenous expression of two gene products from a single promoter element; however, reduced expression of protein from the second cistron compared with the first cistron is a common limitation to this approach. To overcome this limitation, we explored use of dihydrofolate reductase (DHFR) complementary DNA encoded in bicistronic vectors to induce a second protein of interest by methotrexate (MTX) treatment. Previous studies have demonstrated that levels of DHFR protein and DHFR fusion protein can be induced translationally following MTX treatment of cells. We demonstrated that in response to MTX treatment, DHFR partner protein in a bicistronic construct is induced for longer periods of time when compared with endogenous DHFR and DHFR fusion protein, in vitro and in vivo. Using rapamycin pretreatment followed by MTX treatment, we also devised a strategy to modulate levels of two proteins expressed from a bicistronic construct in a cap-independent manner. To our knowledge, this is the first report demonstrating that levels of proteins in DHFR-based bicistronic constructs can be induced and modulated using MTX and rapamycin treatment.
Characterizing Neurons in the Primary Auditory Cortex of the Awake Primate Using Reverse Correlation (1997)

While the understanding of the functional role of different classes of neurons in the awake primary visual cortex has been extensively studied since the time of Hubel and Wiesel (Hubel and Wiesel, 1962), our understanding of the feature selectivity and functional role of neurons in the primary auditory cortex is much farther from complete. Moving bars have long been recognized as an optimal stimulus for many visual cortical neurons, and this finding has recently been confirmed and extended in detail using reverse correlation methods (Jones and Palmer, 1987; Reid and Alonso, 1995; Reid et al., 1991; Ringach et al., 1997). In this study, we recorded from neurons in the primary auditory cortex of the awake primate, and used a novel reverse correlation technique to compute receptive fields (or preferred stimuli), encompassing both multiple frequency components and ongoing time. These spectrotemporal receptive fields make clear that neurons in the primary auditory cortex, as in the primary visual cortex, typically show considerable structure in their feature processing properties, often including multiple excitatory and inhibitory regions in their receptive fields. These neurons can be sensitive to stimulus edges in frequency composition or in time, and sensitive to stimulus transitions such as changes in frequency. These neurons also show strong responses and selectivity to continuous frequency modulated stimuli analogous to visual drifting gratings.
Development of a novel RANKL-based peptide, microglial healing peptide1-AcN (MHP1-AcN), for treatment of ischemic stroke (2018)

Although the regulation of post-ischemic inflammation is an important strategy to treat ischemic stroke, all clinical trials have failed to show its efficacy. To solve the problem, we previously developed a novel partial peptide of RANKL, microglial healing peptide 1 (MHP1), which could reduce ischemic injury by inhibiting Toll-like receptor (TLR) induced inflammation. However, optimization of the peptide was necessary to increase the stability and efficacies for clinical use. According to information gathered through HPLC/MS in serum, we have newly designed a series of modified MHP1 peptides and have found that N-terminal acetylation and C-terminal amidation in MHP1 (MHP1-AcN), can strengthen its anti-inflammatory effects and increase its stability with anti-osteoclastogenic effects. Anti-TLR activity was reported to be reduced in MHP1 when incubated at 37 °C for 24 hrs, but MHP1-AcN could keep the activity under the same condition. The therapeutic effect of MHP1-AcN was observed in transient ischemic stroke model at lower dose than MHP1. Importantly, MHP1-AcN did not affect thrombolytic effects of tissue plasminogen activator (tPA) and inhibited tPA-induced hemorrhagic transformation. These findings indicated that MHP1-AcN was stable and effective anti-TLR signal peptide and could be a promising agent for treating stroke patients receiving tPA and endovascular therapy.
ChemInform Abstract: Pyrazino(2,3‐c)‐1,2,6‐thiadiazine 2,2‐Dioxides. Synthesis of SO2 Analogues of Folic Acid Antagonists. (1991)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Recommendations for HER2 testing in the UK (2000)

Determining the HER2 status of breast carcinomas is a prerequisite for the use of the monoclonal antibody trastuzumab (Herceptin), which has recently been licensed for the treatment of metastatic disease. This necessitates a test based on archival material. The preferred analyses are immunohistochemistry with fluorescent in situ hybridisation (FISH) as a follow up test for ambiguous results. Guidelines have been developed for standardised, well controlled procedures for the provision of reliable results. A group of three reference laboratories has been established to provide advice, quality assurance, and materials, where needed.
Supplementary Figure 1 from The Ovarian Cancer Chemokine Landscape Is Conducive to Homing of Vaccine-Primed and CD3/CD28–Costimulated T Cells Prepared for Adoptive Therapy (2023)

&lt;p&gt;Supplementary Figure 1. Flow cytometry analysis of vaccine-primed and CD3/CD28 costimulated T cells after chemokine-induced migration.&lt;/p&gt;
ChemInform Abstract: A Modular System for Combinatorial and Automated Synthesis (1997)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
High Level, Cell-Specific Expression of Ornithine Decarboxylase Transcripts in Rat Genitourinary Tissues (1989)

We evaluated transcript levels for the rate-limiting enzyme in polyamine biosynthesis, ornithine decarboxylase (ODC), in rat tissues by Northern blotting and in situ hybridization histochemistry, using a rat cDNA probe. ODC transcripts were expressed at a high level, relative to levels in other tissues, in the kidney and testis of the adult rat; maximal levels of transcripts in these tissues occurred after sexual maturation had taken place, i.e. between 20 and 150 days of age. In situ hybridization histochemistry revealed high level expression in the kidney, testis, prostate, and seminal vesicles of the male rat; this high level expression was limited to certain cell types: kidney, S3 cells of the proximal convoluted tubule; prostate and seminal vesicles, glandular or luminal epithelial cells; and testis, early spermatogenic cells. High level expression of ODC mRNA disappeared from the prostate and seminal vesicle epithelial cells after castration and reappeared with testosterone treatment; in contrast, levels of kidney ODC mRNA were essentially unchanged by castration and were similar in male and female adult rats. We conclude that high level ODC mRNA expression occurs in specific cell types in the adult rat, where it appears to be regulated by both androgen-dependent and independent mechanisms.
A near-term design for the SeaView multilevel database system (2003)

The SeaView formal security policy model admits a range of designs for a multilevel secure relational database system. The requirement for a near-term implementation suggests that the design should utilize existing technology to the extent possible. Thus the design uses an existing database management system ported to an existing TCB (trusted computing base) environment. A preprocessor translates key constructs of the SeaView multilevel relational data model to those of the standard relational model used by the commercial database system. The underlying reference monitor enforces mandatory and basic discretionary controls with A1 assurance. By combining single-level data into a multilevel view, it is possible to use a commercial database system and classify data at the relation level to implement the SeaView model, with element-level classification.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
3D mouse embryo model: EMA146, Stage TS21, Age E13.0 (est) (2017)

The eMouseAtlas team have generated a series of 3D images to capture mouse embryo development and to use as a spatial framework for gene-expression and other spatially organised data. The resource is published and available on the Web at http://www.emouseatlas.org/emap/ema/.
Parasiten als Störfaktor bei der Analyse von Biomarkern in ökotoxikologischen Studien (2009)

info:eu-repo/semantics/nonPublished
A Polymorphism Study on Detoxification and DNA Repair Genes in 700 MDS Patients Demonstrates An Association Between Genotype and An Aberrant Karyotype (2008)

Abstract The maintenance of genetic stability within haematopoietic stem cells is essential for normal haematopoiesis and this is emphasised by the association of leukemias and myelodysplastic syndromes (MDS) with genetic instability. DNA is normally protected from damage via a number of complex pathways including detoxification and DNA repair pathways. Inefficient processing of DNA damage may result in an increased susceptibility to leukemia and MDS. Genetic polymorphisms exist in many genes within the DNA damage processing pathways, some of which affect the cells ability to maintain genetic stability. We have studied polymorphisms in the homologous DNA repair genes RAD51 (RAD51-g135c) and XRCC3 (XRCC3-Thr241Met) and the detoxification gene GSTM1 (deletion polymorphism) in more 700 MDS samples. The GSTM1 polymorphism was studied using PCR, and the RAD51 and XRCC3 genotypes were assayed simultaneously using a SNaPshot technique. The genotype distributions of RAD51-g135c and GSTM1 did not differ significantly from those reported in the literature. However the distribution of the XRCC3-Thr241Met polymorphism was found to be significantly different, with an over-representation of the variant Met allele, when compared to previously published frequencies in control populations1 (odds ratio (OR) 1.8; 95% confidence interval (CI) 1.3–2.6, p&amp;lt;0.001). Whilst the presence of a single polymorphic variant may display only a subtle effect, polymorphic variants of more than one gene involved in the same pathway are likely to be biologically important with respect to the cellular ability to maintain genetic integrity and hence may play a role in MDS pathogenesis. RAD51, XRCC3 and GSTM1 genotypes were therefore studied in combined analyses. Similar to studies in AML1, the double DNA repair gene variant (RAD51–135c/XRCC3–241) was over-represented in MDS compared to a control population (OR 3.8; 95% CI 1.6–9.3, p=0.002). The triple variant genotype (RAD51–135c/XRCC3–241Met/GSTM1-null) was associated with a further increased risk of MDS (OR 13.5; 95% CI 1.8–102.8, p=0.01). More detailed analysis was undertaken to compare the polymorphic distributions in MDS with aberrant karyotypes. When the single genes were assessed, the GSTM1 null genotype was the only one to be over-represented in MDS with an aberrant karyotype compared to MDS with a normal karyotype (OR 1.6; 95% CI 1.05–2.5). Interestingly, when analysing the genotypes with respect to the XRCC3/RAD51 combined genotypes the presence of homozygous wild type alleles of one DNA repair gene matched with the presence of a variant allele of the other DNA repair gene is significantly protective against karyotypic abnormalities when compared to the double WT patients (OR 0.29; 95% CI 0.29–0.78; p=0.003). Collectively these results suggest that polymorphisms in genes which process DNA damage play a significant role in MDS pathogenesis and may also contribute to genetic instability in MDS.
The University of Wisconsin-Madison Torsatron/Stellarator Laboratory program, FY 1991--1993 (1991)

This document summarizes results obtained during the first eight months of the current three year grant for research at the University of Wisconsin-Madison Torsatron/Stellarator Laboratory (TSL) and presents plans for future activity during fiscal years 1992 and 1993. Research efforts have focused on fundamental physics issues associated with toroidal confinement, predominantly through experimental investigations on the Interchangeable Module Stellarator (IMS). The program direction has been guided into studies of fluctuations, potentials and electric fields, plasma currents and flows, and effects of magnetic islands by a desire for increased relevance and impact on the general toroidal confinement program. Theoretical and computational activities are also being undertaken to support the experimental research and to identify interesting new toroidal confinement concepts which could contribute to the understanding of tokamak transport.
Differential long-term stability of microRNAs and RNU6B snRNA in 12–20 year old archived formalin-fixed paraffin-embedded specimens (2017)

MiRNA and snRNA levels gradually decreased over an eight year period in FFPE tissue blocks. Sample age was the most consistent feature associated with miRNA stability. The reference snRNA, RUN6B, was more rapidly degraded when compared to miR-141 and miR-221 miRNAs. Various miRNAs demonstrated differential rates of degradation. Quantitative miRNA studies from long-term archived FFPE tissues may therefore benefit from epidemiologic study design or statistical analysis methods that take into account differential storage-dependent transcript degradation.
Influence of Lung Function and Sleep-disordered Breathing on All-Cause Mortality. A Community-based Study (2016)

Lung function was associated with risk for all-cause mortality. The incremental contribution of lung function to mortality diminishes with increasing severity of SDB.