MP81-19 PROGRAMMED DEATH LIGAND-1 (PD-L1) STATUS IN NORTH AMERICAN COHORT OF PENILE SQUAMOUS CELL CARCINOMA (2016)

You have accessJournal of UrologySexual Function/Dysfunction: Penis/Testis/Urethra: Benign Disease & Malignant Disease II1 Apr 2016MP81-19 PROGRAMMED DEATH LIGAND-1 (PD-L1) STATUS IN NORTH AMERICAN COHORT OF PENILE SQUAMOUS CELL CARCINOMA Margaret Cocks, Diana Taheri, Mark W Ball, Stephania M Bezerra, Alan Meeker, Maria Del Carmen Rodriguez, Alcides Chaux, Arthur Burnett, and George Netto Margaret CocksMargaret Cocks More articles by this author , Diana TaheriDiana Taheri More articles by this author , Mark W BallMark W Ball More articles by this author , Stephania M BezerraStephania M Bezerra More articles by this author , Alan MeekerAlan Meeker More articles by this author , Maria Del Carmen RodriguezMaria Del Carmen Rodriguez More articles by this author , Alcides ChauxAlcides Chaux More articles by this author , Arthur BurnettArthur Burnett More articles by this author , and George NettoGeorge Netto More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.2072AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Objective: Penile squamous cell carcinoma (SCC) is primarily treated with surgical resection. Locally advanced and metastatic disease require a multidisciplinary approach. However, mortality and morbidity rates remain high in such patients where a molecular target of therapy is currently sought. Programmed Death Ligand-1 (PD-L1) is a co-inhibitory molecule that impairs the T-cell response by downregulating T-cell proliferation and cytokine production. Tumors cells often upregulate PD-L1 and thereby evade the host immune system. PD-L1 has been successfully targeted in solid tumors. We sought to determine the expression of PD-L1 in penile cancers. METHODS Methods:Fifty-three invasive penile SCCs diagnosed between 1985 and 2013 were retrieved from our surgical pathology archives. Representative formalin-fixed, paraffin-embedded archival blocks were used for the construction of 2 high-density tissue microarrays (TMAs). PD-L1 was investigated by immunohistochemistry using rabbit monoclonal anti PD-L1 antibody (Cell Signaling, E1L3N, 1:100). PD-L1 positivity was defined as presence of any extent (percent) of tumor cell membranous positivity in one or more representative TMA spot. Findings were correlated with clinicopathologic parameters and outcomes by univariate and multivariate analyses. RESULTS Results:Overall 20/53 (38%) of penile SCCs had positive PD-L1 expression. PD-L1 was expressed by a significant proportion of advanced penile SCC. 41% (13/32) of stage pT2 or more SCC and 38% (6/16) of tumors with lymph node metastasis were positive for PD-L1. On univariate analysis, PD-L1 expression did not correlate with patient age, tumor location, histologic subtype, tumor stage, anatomic depth of invasion or tumor grade. PD-L1 positivity did not predict tumor recurrence, progression or cancer related death. On multivariate analysis, only pT stage (p=0.01), presence of lymphovascular invasion (p=0.03), perineural invasion (0.001) and presence of urethral invasion (p=0.008) were significantly associated with cancer related death. CONCLUSIONS Our current study is the largest to assess expression of PD-L1 in clinically annotated North American cohort of penile SCC. PD-L1 was expressed by 38% of penile SCC including those with advanced disease. Our findings support a rational for targeting immune-checkpoint inhibitors pathway in advanced penile SCC. PD-L1 status was not predictive of tumor recurrence, progression or cancer related death. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e1060 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Margaret Cocks More articles by this author Diana Taheri More articles by this author Mark W Ball More articles by this author Stephania M Bezerra More articles by this author Alan Meeker More articles by this author Maria Del Carmen Rodriguez More articles by this author Alcides Chaux More articles by this author Arthur Burnett More articles by this author George Netto More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...