gebhart/patrepeval huggingface dataset:
An alternative membrane topology permits lipid droplet localization of peroxisomal fatty acyl-CoA reductase 1 (2019)

Fatty acyl-CoA reductase 1 (Far1) is a ubiquitously expressed peroxisomal membrane protein that generates the fatty alcohols required for the biosynthesis of ether lipids. Lipid droplet localization of exogenously expressed and endogenous human Far1 was observed by fluorescence microscopy under conditions of increased triglyceride synthesis in tissue culture cells. This unexpected finding was supported further by correlative light electron microscopy and subcellular fractionation. Selective permeabilization, protease sensitivity and N-glycosylation tagging suggested that Far1 is able to assume two different membrane topologies, differing in the orientation of the short hydrophilic C-terminus towards the lumen or the cytosol, respectively. Two closely spaced hydrophobic domains are contained within the C-terminal region. When analyzed separately, the second domain was sufficient for the localization of a fluorescent reporter to lipid droplets. Targeting of Far1 to lipid droplets was not impaired in either Pex19 or ASNA1 (also known as TRC40) CRISPR/Cas9 knockout cells. In conclusion, our data suggest that Far1 is a novel member of the rather exclusive group of dual topology membrane proteins. At the same time, Far1 shows lipid metabolism-dependent differential subcellular localizations to peroxisomes and lipid droplets.
Sub-Hz Closed-Loop Electro-Optomechanical Oscillator with Gallium Phosphide Photonic Crystal Integrated on SoI Circuitry (2023)

We report on a new approach of a low phase noise electro-optomechanical oscillator directly working in the GHz frequency range. The developed nanoscale oscillator is a one-dimensional photonic crystal made of gallium phosphide (GaP), heterogeneously integrated on silicon-on-insulator circuitry. Based on the strong interaction between the optical mode at the telecommunication wavelength and the mechanical mode in GHz, ultra-pure mechanical oscillations are enabled and directly imprinted on an optical carrier. Further stabilization is achieved with a delayed optoelectronic feedback loop using integrated electro-mechanical self-injection. We achieve a short-term stability of 0.7 Hz linewidth and a long-term stability with an Allan deviation below 10<sup>-7</sup> Hz/Hz at 10 s averaging time, which represents an important step toward fully integrated optomechanical oscillators. Integrability and the low phase noise of this oscillator address some of the most important needs of optoelectronic oscillators and pave the way toward on-chip integrated microwave oscillators for microwave applications such as RADARs.
Power combining characteristics of backed-off traveling wave tubes for communications applications (2003)

Traveling wave tubes (TWTs) used in multi-tone and digital communications applications are typically operated backed off from saturation in order to improve the amplifier linearity. This reduces the bit error rate (BER), decreases intermodulation distortion and lowers adjacent channel power; all at the expense of reducing the average output power. For emerging telecommunications applications, the average power requirement is increasing to provide higher bit rates and lower BER, and often exceeds the power obtainable from backed-off space or telecommunications TWTs. One solution is to power combine TWTs that are operated 3 to 10 dB backed off from saturation. Efficient power combining requires that the phase and gain of the signal from each TWT be closely matched. To understand the variation in these parameters across a build-set of tubes, the phase and gain versus drive of 35 Boeing S-band 5525H TWTs were measured. The standard deviation in the phase about the mean phase shift measured at saturation was found to be 2.6/spl deg/, with a related standard deviation of the gain compression at saturation of 0.22 dB. These levels result in small power combining losses and small errors in phased-array fed multi-beam antennas in broadband multi-tone applications.
ChemInform Abstract: CHEMISTRY OF O‐XYLIDENE‐METAL COMPLEXES. PART 3. TUNGSTEN O‐XYLIDENE COMPLEXES DERIVED FROM TETRACHLORO(OXO)TUNGSTEN(VI): X‐RAY CRYSTAL STRUCTURES OF TRIS(O‐PHENYLENEDIMETHYLENE)TUNGSTEN(VI)‐BENZENE (1/0.5) AND BIS(OXOBIS(O‐PHENYLENEDIMETHYLENE)TUNGSTENIO(V))TETRAKIS(TETRAHYDROFURAN)MAGNESIUM (1984)

Abstract Die Reaktion von WOCI, mit der Grignardverbindung (I I) liefert abhängig von den Reaktionsbedingungen das thermisch stabile Chelat ( l) oder den W(V)‐Komplex (III).
Dynamics of ions of intact proteins in the Orbitrap mass analyzer (2009)

While allowing analysis of intact proteins without a theoretical upper mass limit, the Orbitrap mass analyzer demonstrates reduced resolving power as ion mass increases even at a constant mass-to-charge ratio. It is shown that this effect comes from the effects of ion scattering on background gas molecules. The main mechanisms causing decay of acquired transient appear to be fragmentation as well as accelerated dephasing of ion packets. Isotopic resolution of proteins including bovine serum albumin (MW 66.4 kDa) and transferrin (MW 78 kDa) has also been demonstrated. As a part of this study, detection of individual multiply-charged ions of myoglobin (MW 16.9 kDa) has been demonstrated. Quantized distribution of signal intensities for +20 myoglobin ions well above the noise threshold was observed, with high mass accuracy and resolution of recorded individual ions used as an independent confirmation of correct assignment of signal to ions rather than to noise. The latter also allowed us to benchmark the sensitivity of image-current detection and explore in detail factors responsible for signal decay.
<title>Preliminary results of VISX excimer laser myopic photorefractive keratectomy at Cedars-Sinai Medical Center</title> (1992)

Sixty-two eyes underwent excimer laser photorefractive keratectomy (PRK) for the correction of myopia at Cedars-Sinai-Medical-Center. The first group of 12 patients are presented with follow up data of ten months postoperatively. The second group of 50 patients are presented with follow up data of three months postoperatively. An in-depth comparison of pre and postoperative refractive data is presented. Comparisons between pre and postoperative corrected and uncorrected Snellen visual acuities are provided in order to asses the functional visual result of the procedure.
Fair Exchange of Signatures with Multiple Signers (2006)

Chen et al. introduced a new notion of a concurrent signature scheme for a fair exchange of signatures with two parties. Chen et al. also proposed a concrete scheme and proved its security under the assumption of discrete logarithm problem. Recently, Hiwatari and Tanaka extended the concept of concurrent signature to many-to-one setting. Hiwatari and Tanaka also proposed a concrete scheme; however, it requires some strong assumption to achieve the fair exchange and it is not efficient. This paper gives another construction of concurrent signature for many-to-one setting with multisignature scheme. Hereafter, we call it (n,1) concurrent signature scheme. The proposed scheme is more efficient than the scheme of Hiwatari and Tanaka in computation complexity and signature size, and achieves the fair exchange without the assumption required for the scheme of Hiwatari and Tanaka. This paper also gives a construction for the fair exchange of signatures in many-to-many setting, called (n,m) concurrent signature scheme, in appendix.
Statistical Circuit Design: Characterization and Modeling for Statistical Design (1971)

Analysis of the variation in the electrical performance of integrated circuit structures requires a knowledge of the distributions and inter-relationships of device parameter values. This article presents new techniques for more accurate transistor modeling and describes the statistical characterization procedure developed to describe the integrated circuit manufacturing process as far as the measurable electrical parameters are concerned.
Laser Damage of Nanolaminate HfO2 and TiO2 Optical Coatings (2013)

We compared the laser damage performance of uniform versus nanolaminate thin films for hafnia and titania-based coatings. Our experiments showed that the films with higher crystallinity appeared to have better performance for CW systems.
[Oral flecainide in the treatment of refractory arrhythmias. Long-term follow-up of 98 patients]. (1987)

Oral flecainide was administered to 98 patients with arrhythmias regarded as resistant to other antiarrhythmic agents: quinidines (82), propafenone (40), beta-blockers (30), amiodarone alone (38) or combined with a class I compound (19). Therapeutic effectiveness was assessed on clinical date, repeated Holter recordings (64 patients), exercise tests (8) and electrophysiological exploration (15). Mean follow-up was 11.7 +/- 11 months; the patients treated have now been followed up for 18.2 +/- 12 months (range: 7-58 months). Fifty-three patients had atrial arrhythmia (fibrillation or flutter in 45, atrial tachycardia in 8). Flecainide was effective in 26 patients (49%) and ineffective in 27 (51%). There was no significant difference in dosage between these 2 groups: 231 +/- 62 mg/day and 265 +/- 61 mg/day respectively. Paroxysms of re-entrant junctional tachycardia were controlled in 6 of the 8 cases observed. Eleven patients presented with Wolff-Parkinson-White syndrome: treatment was successful in the 3 patients with atrial fibrillation and in 8 of the 10 patients with orthodromic reciprocating rhythms. Among 30 patients with episodes of ventricular tachycardia, 9 (30%) responded to flecainide and 21 (70%) failed to respond. Flecainide reduced the repetitive forms by more than 90% in 7/15 patients and suppressed exercise-induced ventricular tachycardia in 2/8 patients. Fifteen out of 18 patients had ventricular tachycardia reproducible by programmed stimulation; under flecainide, the ventricular tachycardia spontaneously recurred in 4 cases, was provoked by stimulation in 5 other cases, was more easily inducible in 3 cases and was not inducible in a sustained manner in the last 3 cases.(ABSTRACT TRUNCATED AT 250 WORDS)
An underwater wearable computer for two way human-dolphin communication experimentation (2009)

Research in dolphin cognition and communication in the wild is still a challenging task for marine biologists. Most problems arise from the uncontrolled nature of field studies and the challenges of building suitable underwater research equipment. We present a novel underwater wearable computer enabling researchers to engage in an audio-based interaction between humans and dolphins. The design requirements are based on a research protocol developed by a team of marine biologists associated...
Macrophage‐Associated Osteoactivin/GPNMB Mediates Mesenchymal Stem Cell Survival, Proliferation, and Migration Via a CD44‐Dependent Mechanism (2015)

Although MSCs have been widely recognized to have therapeutic potential in the repair of injured or diseased tissues, it remains unclear how functional activities of mesenchymal stem cells (MSCs) are influenced by the surrounding inflammatory milieu at the site of tissue injury. Macrophages constitute an essential component of innate immunity and have been shown to exhibit a phenotypic plasticity in response to various stimuli, which play a central role in both acute inflammation and wound repair. Osteoactivin (OA)/Glycoprotein non-metastatic melanoma protein B (GPNMB), a transmembrane glycoprotein that plays a role in cell differentiation, survival, and angiogenesis. The objective of this study was to investigate the potential role of OA/GPNMB in macrophage-induced MSC function. We found that reparative M2 macrophages express significantly greater levels of OA/GPNMB than pro-inflammatory M1 macrophages. Furthermore, using loss of function and rescue studies, we demonstrated that M2 macrophages-secreted OA/GPNMB positively regulates the viability, proliferation, and migration of MSCs. More importantly, we demonstrated that OA/GPNMB acts through ERK and AKT signaling pathways in MSCs via CD44, to induce these effects. Taken together, our results provide pivotal insight into the mechanism by which OA/GPNMB contributes to the tissue reparative phenotype of M2 macrophages and positively regulates functional activities of MSCs. J. Cell. Biochem. 117: 1511-1521, 2016. © 2015 Wiley Periodicals, Inc.
Stakeholder engagement to ensure the sustainability of biobanks: a survey of potential users of biobank services (2021)

Biobanks are important infrastructures facilitating biomedical research. After a decade of rolling out such infrastructures, a shift in attention to the sustainability of biobanks could be observed in recent years. In this regard, an increase in the as yet relatively low utilisation rates of biobanks has been formulated as a goal. Higher utilisation rates can only be achieved if the perspectives of potential users of biobanks-particularly researchers not yet collaborating with biobanks-are adequately considered. To better understand their perspectives, a survey was conducted at ten different research institutions in Germany hosting a centralised biobank. The survey targeted potential users of biobank services, i.e. researchers working with biosamples. It addressed the general demand for biosamples, strategies for biosample acquisition/storage and reasons for/against collaborating with biobanks. In total, 354 researchers filled out the survey. Most interestingly, only a minority of researchers (12%) acquired their biosamples via biobanks. Of the respondents not collaborating with biobanks on sample acquisition, around half were not aware of the (services of the) respective local biobank. Those who actively decided against acquiring biosamples via a biobank provided different reasons. Most commonly, respondents stated that the biosamples required were not available, the costs were too high and information about the available biosamples was not readily accessible. Biobanks can draw many lessons from the results of the survey. Particularly, external communication and outreach should be improved. Additionally, biobanks might have to reassess whether their particular collection strategies are adequately aligned with local researchers' needs.
Simplified Inverse Method for Determining the Tensile Strain Capacity of Strain Hardening Cementitious Composites (2007)

As emerging advanced construction materials, strain hardening cementitious composites (SHCCs) have seen increasing field applications recently to take advantage of its unique tensile strain hardening behavior, yet existing uniaxial tensile tests are relatively complicated and sometime difficult to implement, particularly for quality control purpose in field applications. This paper presents a new simple inverse method for quality control of tensile strain capacity by conducting beam bending test. It is shown through a theoretical model that the beam deflection from a flexural test can be linearly related to tensile strain capacity. A master curve relating this easily measured structural element property to material tensile strain capacity is constructed from parametric studies of a wide range of material tensile and compressive properties. This proposed method (UM method) has been validated with uniaxial tensile test results with reasonable agreement. In addition, this proposed method is also compared with the Japan Concrete Institute (JCI) method. Comparable accuracy is found, yet the present method is characterized with much simpler experiment setup requirement and data interpretation procedure. Therefore, it is expected that this proposed method can greatly simplify the quality control of SHCCs both in execution and interpretation phases, contributing to the wider acceptance of this type of new material in field applications.
Design of GEMSTAR tidal current fixed pitch rotor controlled through a Permanent Magnet Generator (PMG) de‐fluxing technique (2020)

This work reports a study on a tidal current system named GEMSTAR, composed of two hydrokinetic turbines supported by a floating submerged structure. A flexible mooring cable links the floating support to the seabed allowing the free rotation of the system and its alignment to changing current direction. The work is mainly focused on the design of the turbine blade with two objectives: keeping a constant power output above the rated power condition and limiting the thrust increase between rated and maximum operating speed. A fixed pitch blade was considered, in order to reduce the costs associated to a complex pitch control mechanism. An analytical procedure for blade design is proposed and validated by comparison to other numerical methods. Above the rated current speed, the power is held constant while the rotational speed of the turbine and of the generator increases, assuming an over‐speeding control strategy. The adopted design configuration relies on the possibility of the generator to operate in de‐fluxing working conditions. As reported in the last part of this study, a numerical model of the generator has also been developed and applied to a test case in order to analyse the electric behaviour of the system.
Protease susceptibility and toxicity of heat-labile enterotoxins with a mutation in the active site or in the protease-sensitive loop (1997)

To generate nontoxic derivatives of Escherichia coli heat-labile enterotoxin (LT), site-directed mutagenesis has been used to change either the amino acid residues located in the catalytic site (M. Pizza, M. Domenighini, W. Hol, V. Giannelli, M. R. Fontana, M. M. Giuliani, C. Magagnoli, S. Peppoloni, R. Manetti, and R. Rappuoli, Mol. Microbiol. 14:51-60, 1994) or those located in the proteolytically sensitive loop that joins the A1 and A2 moieties of the A subunit (C. C. R. Grant, R. J. Messer, and W. J. Cieplack, Infect. Immun. 62:4270-4278, 1994; B. L. Dickinson and J. D. Clements, Infect. Immun. 63:1617-1623, 1995). In this work, we compared the in vitro and in vivo toxic properties and the resistance to protease digestion of the prototype molecules obtained by both approaches (LT-K63 and LT-R192G, respectively). As expected, LT-K63 was normally processed by proteases, while LT-R192G showed increased resistance to trypsin in vitro and was digested by trypsin only under denaturing conditions (3.5 M urea) or by intestinal proteases. No toxicity was detected with the LT-K63 mutant, even when 40 micrograms and 1 mg were used in the in vitro and in vivo assays, respectively. In marked contrast, LT-R192G showed only a modest (10-fold) reduction in toxicity in Y1 cells with a delay in the appearance of the toxic activity and had toxicity comparable to that of wild-type LT in the rabbit ileal loop assay. We conclude that mutagenesis of the active site generates molecules that are fully devoid of toxicity, while mutagenesis of the A1-A2 loop generates molecules that are resistant to trypsin in vitro but still susceptible to proteolytic activation by proteases other than trypsin, and therefore they may still be toxic in tissue culture and in vivo.
Amino Acid Bromides:  Their N-Protection and Use in the Synthesis of Peptides with Extremely Difficult Sequences. (2003)

ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionORIGINAL ARTICLEThis notice is a correctionAmino Acid Bromides: Their N-Protection and Use in the Synthesis of Peptides with Extremely Difficult Sequences.Alma DalPozzo, Minghong Ni, Laura Muzi, Andrea Caporale, Roberto de Castiglione, Bernard Kaptein, Quirinus B. Broxterman, and Fernando FormaggioCite this: J. Org. Chem. 2003, 68, 6, 2528Publication Date (Web):February 19, 2003Publication History Published online19 February 2003Published inissue 1 March 2003https://pubs.acs.org/doi/10.1021/jo039926qhttps://doi.org/10.1021/jo039926qcorrectionACS PublicationsCopyright © 2003 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views382Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (17 KB) Get e-Alertsclose Get e-Alerts
Clonal expansions of Vδ1+ and Vδ2+ cells increase with age and limit the repertoire of human γδ T cells (1994)

We have investigated the complexity of the human gamma delta T cell repertoire by means of a VJ heteroduplex analysis method. cDNA obtained from peripheral blood mononuclear cells was amplified with V delta 1-C delta or V delta 2-C delta primers. The product was denatured and renatured to allow random reannealing of the strands and the heteroduplexes carrying mismatched junctional sequences were separated from the homoduplexes on polyacrylamide gels. Whenever one or more T cell clones were expanded to over 10% of the polyclonal background, discrete bands of homo- and heteroduplex appeared. This method was applied to the analysis of the peripheral gamma delta compartment from healthy donors and rheumatoid arthritis patients of different ages. While samples from young individuals showed a polyclonal pattern, a clear tendency towards oligoclonality appeared with increasing age, both in normal individuals and rheumatoid arthritis patients. We also show that the VJ junctional sequence derived from the heteroduplex fragments can be successfully used to isolate and characterize the corresponding T cell clones in vitro, even after a period of 1 year. In conclusion, our findings indicate that the complexity of the gamma delta T cell repertoire decreases with age as a consequence of the expansion of a few T cell clones.
Optimisation du rapport débit-distorsion de la compression progressive de maillages par adaptation de quantification (2010)

National audience
Procollagen II Gene Mutation in Stickler Syndrome (1992)

Four affected members of a family with Stickler syndrome were found to have a single base-pair deletion resulting in a translational frameshift in exon 40 of the procollagen II (COL2A1) gene on chromosome 12. This mutation was not seen in any of five clinically unaffected family members or in any of 15 unrelated control patients. All affected members had distinctly abnormal vitreous syneresis and all had retinal perivascular pigmentation. Retinal detachments occurred in three of the four affected patients. Three of the four affected patients had peripheral cortical "wedge" cataracts, and the fourth had extensive nuclear sclerosis. Abnormalities of the soft palate were found in all four affected patients. All patients reported severe joint pains, and epiphyseal dysplasia was found radiographically in all patients.
Metal ferrite spinel energy storage devices and methods for making and using same (2023)

1-100 nm metal ferrite spinel coatings are provided on substrates, preferably by using an atomic layer deposition process. The coatings are able to store energy such as solar energy, and to release that stored energy, via a redox reaction. The coating is first thermally or chemically reduced. The reduced coating is then oxidized in a second step to release energy and/or hydrogen, carbon monoxide or other reduced species.