gebhart/patrepeval huggingface dataset:
Isolation and mapping of EVx1, a human homeobox gene homologus toeven-skipped, localized at the 5′ end of Hox1 locus on chromosome 7 (1991)

We isolated and mapped the human homeobox gene EVX1. This gene encodes a protein of 407 amino acid residues containing a homeodomain closely related to the Drosophila even-skipped (eve) segmentation gene of the pair-rule class. EVX1 belongs to a small family of vertebrate eve-related homeobox genes including human EVX1 and EVX2 genes, their murine homologs, Evx 1 and Evx 2, and the frog Xhox-3 gene. We previously reported that EVX2 is localized at the 5' end of the HOX4 locus on chromosome 2. We show here that EVX1 is localized at the 5' end of the HOX1 locus on chromosome 7, 48 kb upstream from the most 5' of the eleven HOX1 genes, namely HOX1J. Both EVX genes are transcribed in an opposite orientation as compared to that of adjacent HOX genes. Human HOX1 and HOX4 complex loci appear to be both closely linked to a homeobox gene of the EVX family.
Étude de la réalisation de matériau silicium sur isolant (SOI) à partir du collage par adhésion moléculaire silicium sur oxyde (procédé Smart-Cut®) (1997)

Le materiau SOI presente de nombreux avantages pour les composants basse consommation et basse tension, ce qui le positionne comme materiau de base pour les applications microelectroniques futures. Le nouveau procede Smart-Cut®, permettant la fabrication de materiau SOI, combine deux technologies de base : l'implantation d'hydrogene et le collage par adhesion moleculaire, assurant ainsi la possibilite de production de fort volume a faible cout. Cette these traite de l'etape de collage par adhesion moleculaire, du recuit haute temperature de la structure SOI et des caracteristiques du materiau fini. Les mecanismes de formation des defauts macroscopiques lors du transfert du film SOI ont ete identifies ; il a alors ete possible de developper une sequence de nettoyage avant collage optimisee, evitant la formation de ces defauts. La mise en œuvre d'un outil specifique permettant une analyse par reflexions internes multiples, en spectroscopie infrarouge des structures collees, a permis d'observer l'evolution chimique de l'interface de collage dans le cas de collages silicium/silicium, silicium/oxyde thermique et silicium/oxyde thermique implante. La correlation de ces resultats microscopiques avec une caracterisation macroscopique de l'energie du collage a mene a la comprehension des mecanismes de collage. Les specificites du recuit haute temperature et de l'oxydation de la structure SOI ont ete apprehendees. Cette etape de recuit a alors ete optimisee. Le materiau SOI-Unibond® alors obtenu est compatible avec les besoins de l'industrie ULSI
Optochemical Control of Protein Localization and Activity within Cell-like Compartments (2018)

We report inducible dimerization strategies for controlling protein positioning, enzymatic activity, and organelle assembly inside synthetic cell-like compartments upon photostimulation. Using a photocaged TMP-Haloligand compound, we demonstrate small molecule and light-induced dimerization of DHFR and Haloenzyme to localize proteins to a compartment boundary and reconstitute tripartite sfGFP assembly. Using photocaged rapamycin and fragments of split TEV protease fused to FRB and FKBP, we establish optical triggering of protease activity inside cell-size compartments. We apply light-inducible protease activation to initiate assembly of membraneless organelles, demonstrating the applicability of these tools for characterizing cell biological processes in vitro. This modular toolkit, which affords spatial and temporal control of protein function in a minimal cell-like system, represents a critical step toward the reconstitution of a tunable synthetic cell, built from the bottom up.
Phospholipase A2 is a differentiation-dependent enzymatic activity for adipogenic cell line and adipocyte precursors in primary culture. (1990)

Phospholipase A2 enzymatic activity was measured in the teratoma-derived adipogenic cell line 1246 and in adipocyte precursors in primary cultures. It was shown that enzymatic activity was low while the cells were undifferentiated and increased by 20-24-fold after the cells had undergone adipocyte differentiation. The increase of phospholipase A2 activity follows the same time course as that observed for glycerol-3-phosphate dehydrogenase activity used as a marker of differentiation. In contrast, the differentiation-deficient, insulin-independent cell line 1246-3A always contained very low levels of phospholipase A2 activity. Phospholipase A2 activity measured in the 1246 cells was inhibited in a dose-dependent fashion by incubation with ONO-RS-082 and quinacrine which are inhibitors of phospholipase A2 activity. Measurements of arachidonate metabolites in 1246 cells showed that production of prostaglandin F2 alpha by the 1246 cells followed the same time course as the increase of phospholipase A2 activity during differentiation. Similar results were obtained with primary cultures of adipocyte precursors. These results indicate that phospholipase A2 is a differentiation-dependent enzymatic activity for the adipogenic cell line 1246 and for adipocyte precursors in primary culture. These data suggest that metabolic pathways controlled by phospholipase A2 activity could play an important physiological role in adipose tissue differentiation.
Where they fell: The 164 Lancasters lost from East Kirkby (2016)

164 Lancaster bombers and thousands of airmen were lost from RAF East Kirkby in WW2. ThereÃ¢â‚¬â„¢s now a museum at the airfield Ã¢â‚¬â€œ The Lincolnshire Aviation Heritage Centre Ã¢â‚¬â€œ dedicated to remembering those times.This exhibition maps all of the aircraft and people lost in action from this site. This is a story about tremendous effort and sacrifice at a dark time in history, where many airfields like this one acted as a fighting front in the Second World War. The exhibition acts as a visual system to encourage and help museum visitors experience the museums' extensive collection of archive material and artefacts that tell the story of these lost aircrews and their exploits. The display exists within the control tower which still stands at the airfield Ã¢â‚¬â€œ from where all of this action happened.
Inheritance of an epigenetic change in the mouse: a new role for RNA (2007)

Hereditary epigenetic variation, initially recognized and studied extensively in plants, had not been reported in mammals until recently. We have now identified the Kit locus as the first example of a paramutable gene of the mouse. Kit(+/+) homozygotes born from Kit(tm1Alf)(/+) heterozygotes maintain and transmit to their progeny the white-spotted phenotype characteristic of the mutant heterozygote. Our observation of unusual amounts of RNA in the sperm of the paramutated (Kit*) males had led us to consider the possibility of RNA-mediated inheritance. A role for RNA was supported further by the efficient establishment of the epigenetic modification following microinjection in one-cell embryos of either sperm RNA of the paramutated males or of the Kit-specific microRNAs miR-221 and -222. In this article, we describe the phenotypes associated with the wild-type genome in the Kit* paramutated animals. Paramutation may be considered to be one possibility of epigenetic modification in the case of familial disease predispositions that are not fully accounted for by Mendelian analysis.
A Brief Overview of Nanoelectronic Devices (1998)

A prospective study was performed to establish the frequency of contact allergy in atopic patients presenting with dermatitis, compared with non-atopics suffering from dermatitis. During 1987-1988, all new patients aged 15 years or older, who consulted us for dermatitis, were investigated. They were patch tested with the European standard series, methyl(chloro)isothiazolinone and other relevant allergens. In addition, they were prick tested with 24 inhalant and 11 food allergens. Patients having at least 2 positive prick tests, and patients with 1 positive prick test and a (family) history of atopic diseases were defined as atopics, as were those who presented with classic atopic dermatitis but with negative prick tests. 499 patients were evaluated: 159 men and 340 women. 214 patients (43%) were atopic, the other 285 (57%) were non-atopic. In the atopic group, 79 persons (37%) had at least 1 positive patch test reaction. In the group of non-atopics, 149 patients (52%) had contact allergies. The difference is statistically significant (chi 2; p less than 0.05). It is concluded that adult atopics seen in dermatological practice who present with dermatitis are less frequently contact sensitized than such patients who are non-atopic. Nevertheless, a yield of nearly 40% positive patch test reactions in this group still makes routine patch testing necessary.
Histone deacetylase inhibitors induce growth suppression and cell death in human rhabdomyosarcoma in vitro. (2003)

SAHA and pyroxamide induce growth suppression and cell death in human RMS in vitro. Accumulation of acetylated histones and induction of p21/WAF1 expression are observed in cells exposed to either agent.
Increases in muscle Ca2+ mediate changes in acetylcholinesterase and acetylcholine receptors caused by muscle contraction. (1985)

The synthesis of acetylcholinesterase (AcChoE; acetylcholine acetylhydrolase, EC 3.1.1.7) and of acetylcholine receptors (AcChoR) by cultured rat muscle fibers is influenced strongly by the level of muscle contractile activity. If fibers are grown in the presence of tetrodotoxin (TTX) to block spontaneous contraction, the total amount of AcChoE decreases markedly, as does the percentage of AcChoE assembled as the collagen-tailed presumed synaptic form of the enzyme. Under these conditions, however, the number of AcChoR increases. We demonstrate here that each effect of TTX can be prevented by treating the muscle cells with the calcium ionophore A23187. Thus, cells treated with A23187 and TTX have 30- to 40-fold higher levels of collagen-tailed AcChoE and lower levels of AcChoR by a factor of 4-5 than do cells grown in TTX alone. These results suggest that an increase in muscle cytoplasmic Ca2+ mediates the known effects of muscle contraction on these cholinergic macromolecules.
Influence of the Activation Catalyst on the Polarization Resistance of Electroless Cu Electrodes on BaCe0.1Zr0.8Y0.1O3-δ proton-Conducting Ceramics (2017)

Copper (Cu) electrodes appear as potential methane dehydroaromatization (MDA) electrodes on BaCe x Zr 0.9-x Y 0.1 O 3- δ (BCZY) proton-conducting ceramics [1]. When applied from paste, the electrodes suffer from poor adhesion, and therefore high polarization resistance. In this work, 1 micron thin Cu electrodes were deposited onto BaCe 0.1 Zr 0.8 Y 0.1 O 3-d (BCZY18) specimens by electroless plating, using three different activation catalysts: Pd, Ru and Cu [2]. Impedance spectra were recorded on electrolyte based symmetric cells Cu/BCZY/Cu in the temperature range 750-600°C, in dry and moist reducing atmospheres. The area specific resistances (ASR) of these three electrodes were found to be lower in moist gases and were two orders of magnitude lower than that measured on painted Cu electrodes (see figure) . At 700°C, in 3% moist forming gas, ASR of 2.9, 3.8 and 22.7 Ω∙cm 2 were calculated on the Cu electrodes with the Pd, Ru and Cu activation catalyst respectively. The microstructure of the electrodes was evaluated before and after electrochemical testing. In all cases, nano-pores were present on the as-deposited electrodes. After exposure to dry and moist reducing atmosphere at high temperatures, the microstructure of the Pd/Cu and Ru/Cu did not change significantly while major reorganisation was observed for the Cu/Cu electrode. [1] Morejudo SH, Zanón R, Escolástico S, Yuste-Tirados I, Malerød-Fjeld H, Vestre PK, et al. Direct conversion of methane to aromatics in a catalytic co-ionic membrane reactor. Science 2016;353:563–566 [2] Patki N, Ricote S, Way JD, Fabrication of reducing atmosphere electrodes (fuel electrodes) by electroless plating of copper on BaZr 0.9-x Ce x Y 0.1 O 3-δ – a proton-conducting ceramic, submitted to International Journal of Hydrogen Energy in November 2016 Figure 1
Crystal structure of Tris(N,N-dibutyldithiocarbamato)iron(III) benzene solvate (1976)

The crystal structure of the title compound, [Fe(CS2NBu2)3],C6H6, has been determined at 295 K from diffractometer data and refined by least squares to a residual of 0.071 for 3077 'observed' reflections. Crystals are orthorhombic, P ncn , a = 23.660(9), b = 17.331(7), c = 10.349(9) Å. Unlike the unsolvated derivative, the present compound has a magnetic moment of 3.6 B.M. at 295 K. Its molecular symmetry is 2, (FeS) being 2.34, Å. The role of the benzene solvent, at a lattice site, is not indicative of any strong intermolecular interaction.
EXTH-44. PERSONALIZED IMMUNOTHERAPY VACCINES FOR GLIOBLASTOMA AND THEIR TREATMENT EFFICACY (2022)

Abstract BACKGROUND Glioblastoma multiforme (GBM) remains a disease with debilitating survival outcomes. Cancer immunogenomics represents a complementary approach to the application of genomics in developing novel immunotherapies for brain malignancies. Using a cancer immunogenomics approach that we developed, called the Open Reading Frame Antigen Network (O.R.A.N.), we identified the immunogenic neoantigens and tumor-associated antigens (TAAs) including cancer-testis and developmental antigens, that are aberrantly overexpressed in murine models of GBM. The aim of this study is to evaluate the immunologic effects and safety of immunotherapy vaccines targeting neoantigens and TAAs in preclinical models of GBM. METHODS RNAseq and WES were performed for murine glioblastoma tumors- KR158-Luc and GL261, and the results were fed to the O.R.A.N pipeline for antigen prediction. A tumor antigen-specific RNA library was created for each tumor using a gene enrichment strategy and validated by RNAseq. Tumor-bearing animals were treated with adoptively transferred ex vivo expanded lymphocytes and dendritic cell vaccines loaded with the tumor antigen-specific RNA. Tumor volume, and thus progress, was determined using in vivo luciferase imaging technique and the survival outcomes were noted. We also evaluated the efficacy of the tumor vaccines in combination with checkpoint blockade therapy by treating tumor-bearing animals with dendritic cell vaccines loaded with the tumor antigen-specific RNA combined with an anti-PD-1 antibody. RESULTS The dendritic cell vaccines loaded with tumor antigen-specific RNA were significantly effective in slowing the progression of murine GBM tumors in combination with both the adoptive cellular therapy as well as checkpoint blockade therapy. Additionally, we identified antigen-specific T cells targeting several of our predicted antigens and an increase in tumor-infiltrating lymphocytes and memory T cells in the treated animals. CONCLUSION We developed a dendritic cell-based vaccination approach targeting neoantigens and TAAs identified as being tumor-specific and evaluated the efficacy of immunotherapy vaccines in preclinical models of GBM.
AI privacy preserving robots working in a smart sensor environment (2022)

For enhanced safety, autonomous Guided Vehicles (AGV) in warehouses complement their local sensors with data from environmental sensors to improve the detection of humans not in view of the AGV. The problem with many existing approaches is that they broadcast all environmental data in plain, leading to both privacy and bandwidth issues. In our privacy-preserving amalgamated machine learning approach, the environment sensors do data pre-processing and only send abstracted features to the AGVs, solving both privacy and bandwidth problems. The AGVs use these abstracted features in an internal machine learning model to quantify the safety-level of the environment. We have implemented a physical demonstrator using minimal hardware, following this principle. Our demo AGV ran positioning, navigation and the safety inference in real time on a simple Raspberry Pi, solely relying on the amalgamated features to avoid both static and dynamic objects. By only broadcasting abstract features, the safety level can be maintained, the privacy is preserved, and the used bandwidth is reduced by more than 2 orders of magnitude, compared to sharing all video streams.
Avis en réponse à la saisine du 7 novembre 2013, de Madame Marie-Christine Blandin, relative à l’article de Snell et al. (Food and Chemical Toxicology, 2012) (2014)

Le Haut Conseil des biotechnologies (HCB) a été saisi le 7 novembre 2013 par Madame la Sénatrice Marie-Christine Blandin, en vertu de l’article L531-3 du code de l’environnement, d’une demande d’avis relative à l’article de Snell et al., intitulé «Assessment of the health impact of GM plant diets in long-term and multigenerational animal feeding trials: A literature review», publié dans la revue Food and Chemical Toxicology (Snellet al.,2012). Pour répondre aux questions de la saisine, le Comité Scientifique (CS) du HCB a constitué un groupe de travail ad hoc. A la suite du compte-rendu de ce dernier, le CS du HCB a procédé à l’examen du projet de réponse le 25 février 2014 sous la présidence de Jean-Christophe Pagès.
Configurable Module Topology to Recover Power Lost due to Current Mismatch (2014)

Variable shading due to clouds and nearby objects has a severe impact on the energy-yield of photovoltaic installations. Due to the industry's standard of permanently series-connected cells in a PV module, partial shading creates mismatches between the I-V characteristics of cells. Apart from irradiation, temperature differences can lead to non-uniform operating conditions of the module. Current mismatch in series-connected cells reduces the total power either by limiting the extracted current or by bypassing “poorly” operating cells. Our proposal is a configurable module topology, where cell connections can be decided and altered during operation. A network of switches allows cells to be clustered at run-time based on their instantaneous MPP. Cost of additional components is taken into account and the parameters of cells, wires and switches included in the simulation model are taken from fabrication data. The proposed configurable topology can recover most of the power lost due to current mismatch and outperforms conventional topologies under non-uniform operating conditions, while the cost remains acceptable. In certain shading patterns with heavy shade, configurable topologies can produce double the power than conventional PV modules.
Superconductivity in the Organic Charge Transfer Salts: (TMTSF)2X and (TMTTF)2X (1982)

Abstract We report pressure dependent studies of the a-axis resistivity as a function of temperature for several members of the isostructural families of organic charge transfer salts, (TMTSF)2X and (TMTTF) 2X. For a typical (TMTSF)2X material the low temperature metal-insulator transition seen at 1 bar is suppressed above some critical pressure, Pc, where a superconducting transition is observed near 1 K. We find a correlation between Pc and the ambient pressure c lattice parameter which reflects the anion size. The (TMTTF) cX salts exhibit very different ambient pressure behaviour but we find that with the application of sufficiently high pressures (∼30 kbar) their behaviour resembles that seen in the (TMTSF)2X family but at lower pressures. In particular we find evidence of a possible superconducting transition near 4 K in (TMTTF)2Br at 25 kbar. At this pressure the conductivity near 4 K is extremely high with a value approaching 106 (Ωcm)−1 and the resistivity ratio is about 400.
Supplementary Tables 1 - 2, Figures 1 - 2 from Emergence of Constitutively Active Estrogen Receptor-α Mutations in Pretreated Advanced Estrogen Receptor–Positive Breast Cancer (2023)

&lt;p&gt;PDF file - 187K, Supplementary Table 1: Detailed demographic information of all patients with metastatic breast cancer, including cohorts LM+ and EM+. Supplementary Table 2: ESR1 variant sequence data details. Abbreviations: cvg, coverage, N/A, non-applicable. Supplementary Table 3: List of genes sequenced by captured next generation sequencing. Supplementary figure 1: Genomic profiles of primary and metastatic tumors. Genomic alterations were found in 32 genes in primary and metastatic ER+ breast cancers. Genes are listed from the most frequently altered to least altered gene. Supplementary figure 2: No change in WT and mutant ER activity across a wide range of E2 doses. Luciferase activity in 293T cells after co-transfection of the ERE-TK-Luc reporter vector along with WT-ER, Y537N or D538G and E2 stimulation using doses of E2 ranging from 0.01nM to 100nM or vehicle treatment.&lt;/p&gt;
(Invited) Using Industry to Inspire Academic Research (2016)

Market driven applications may benefit from improved or next generation products. However, the scope of the product modification for the new application may be substantial, require significant investment and be high risk. Thus, the new product introduction may be beyond the risk tolerance of a company even though the application and product are aligned with company objectives. This scenario provides an opportunity for academically based research. Academic research can pursue higher risk alternatives in parallel with the investigation of the basic science phenomena associated with the new concept. Several case studies will be described. For example, one case focuses on the investigation of a new class of active cathode materials for an implantable medical battery. A known life limiting phenomena, cathode solubility, was explored at a fundamental level for the benchmark commercialized system and compared with the new class of materials under development. It was demonstrated that the life limiting mechanism of the battery could be significantly reduced. The second case study describes improvement in pulse power for a lithium/carbon monofluoride (CFx) battery. While the Li/CFx system provides high energy density, it has been limited by its power capability. Electrode design approaches were utilized to address the limitation to broader market adoption.
Movie 2 from Metabolic Screening of Cytotoxic T-cell Effector Function Reveals the Role of CRAC Channels in Regulating Lethal Hit Delivery (2023)

&lt;p&gt;OT-I CTLs engaging with and detaching from B16F10/OVA cells in 2D matrix-free or 3D interface co-culture. Note the frequent instable interactions in 2D matrix-free co-culture but not in 3D interface culture. Time stamp, h:min:sec. Scale bar, 10 µm.&lt;/p&gt;
Effect of Martensite in Initial Structure on Bainite Transformation (2010)

Lath-shaped upper bainite structures play a very important role in many high-strength steels (HSSs) and ultra high-strength steels (UHSSs). Although bainite transformation is strongly affected by the initial structure, the effect of the second phase in a multi-phase structure is yet to be clearly understood. It is significant for the advancement of UHSS to study this effect. The aim of this study is to clarify the effect of martensite, which forms before bainite, in Fe-0.2C-8Ni alloy. The bainite transformation from an austenite and martensite dual-phase structure is faster than that from single-phase austenite and the nucleation of bainitic ferrite laths are accelerated around martensite. This effect of martensite on bainite kinetics is equivalent to that of polygonal ferrite when their volume fractions are almost the same. This suggests that the boundary between martensite and austenite is a prior nucleation site of bainitic ferrite. Martensite also affects the crystallographic features of bainite. The orientations of bainitic ferrite laths tend to belong to the same block with martensite adjacent. This tendency intensifies with an increase of the transformation temperature of bainite, resulting in the formation of huge blocks consisting of bainitic ferrite and martensite laths at high temperatures (693K and 723K). In contrast, at a low temperature (643K), bainitic ferrite laths belong to same packet as martensite and have several orientations. This change of crystallographic features with transformation temperature can explain with the driving force of the nucleation of bainitic ferrite.