gebhart/patrepeval huggingface dataset:
Expression of SKP1 mRNA and protein in rat brain during postnatal development (1997)

We previously isolated the cell cycle element SKP1 as a candidate plasticity gene in the rat visual cortex. Here, we studied the expression and localization of SKP1 mRNA and protein in rat brain. We found a high level of expression for the SKP1 gene in the cortex at different postnatal ages. SKP1 mRNA levels remained stable from P2 (postnatal day 2) to adulthood. SKP1 mRNA expression was also detected in several other brain areas. Skp1p (SKP1 protein) immunohistochemistry showed nuclear staining in a large majority of neurones. The pyramidal cells in the hippocampus, as well as cortical cells were stained. The presence of Skp1p in post-mitotic neurones suggests that this protein is involved in processes other than the cell cycles other target proteins and functions in neurones are currently under investigation.
Erratum: “Voltage induced magnetostrictive switching of nanomagnets: Strain assisted spin transfer torque random access memory” [Appl. Phys. Lett. 104, 262407 (2014)] (2015)

First Page
The first determination of carbonyl oxide geometry. Effect of ring size on the stereochemistry of carbonyl oxides from ozonolysis of cycloalkenes (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe first determination of carbonyl oxide geometry. Effect of ring size on the stereochemistry of carbonyl oxides from ozonolysis of cycloalkenesWilliam H. Bunnelle and Sang Gi LeeCite this: J. Am. Chem. Soc. 1992, 114, 19, 7577–7578Publication Date (Print):September 1, 1992Publication History Published online1 May 2002Published inissue 1 September 1992https://pubs.acs.org/doi/10.1021/ja00045a048https://doi.org/10.1021/ja00045a048research-articleACS PublicationsRequest reuse permissionsArticle Views124Altmetric-Citations13LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
DNA Modification by 4-Aza-3-ene-1,6-diynes:  DNA Cleavage, pH-Dependent Cytosine-Specific Interactions, and Cancer Cell Cytotoxicity (2002)

The (Z)-hex-1,5-diyne-3-ene reactive core common to the enediyne antitumor antibiotics undergoes a Bergman cyclization after proper activation to afford reactive diradical intermediates that are responsible for initiating DNA cleavage. Direct modification of the enediyne core has been proposed as a method to permit cancer cell-specific triggering of the diradical-generating cyclization. For example, 3-aza-3-ene-1,5-diynes undergo an aza-Bergman cyclization to afford the fleeting 2,5-didehydropyridine diradicals. While protonation of these aza-enediynes can afford products of diradical trapping, the hydrolytic instability of the 3-aza-3-ene-1,5-diyne moiety prevents its use in pH-triggered DNA cleaving anticancer agents. Recently, more hydrolytically stable systems incorporating the 4-aza-3-ene-1,6-diyne moiety were developed. We report here studies of the 4-aza-3-ene-1,6-diyne-containing benzimidazolium salt AZB002 [1-methyl-2-(phenylethynyl)-3-(3-phenylprop-2-ynyl)-3H-benzimidazolium tetrafluoroborate] and two structurally related heterocycles that lack the aza-enediyne functionality, AZB016 [1,3-dimethyl-2-(phenylethynyl)-3H-benzimidazolium triflate] and AZB004 [3-methyl-2-(phenylethynyl)benzothiazolium triflate]. The interaction of these compounds with supercoiled DNA, a double-stranded DNA fragment, and a short DNA duplex oligonucleotide was investigated. There are three distinct DNA interactions exhibited by AZB002: a frank strand scission leading to the relaxation of supercoiled DNA and formation of at least two different DNA adducts, one of which leads to cytosine-specific cleavage after piperidine/heat treatment. In contrast, analogues lacking the aza-enediyne functionality either fail to interact with DNA (AZB016) or cleave DNA at guanine residues, presumably through alkylation of the N-7 position (AZB004). We also investigated the cytotoxicity of AZB002 and the related heterocyclic compounds AZB004 and AZB016 and find that only the DNA interactive compounds AZB002 and AZB004 display significant cytotoxicity. In particular, AZB002 is cytotoxic against a wide range of cancer cell lines.
Frequency control of a laser diode by a photothermal effect and its application to frequency stabilization (1994)

The frequency of an AlGaAs laser diode (LD) was controlled by using a photothermal effect. If intensity-modulated light from a heating LD is focused on the active domain of another LD, a heat spot changes the effective cavity length of the LD and thus modulates the lasting frequency. For frequency stabilization, frequency shift is detected by a Fabry-Pérot etalon, and it is compensated for by controlling the intensity of the heating LD via a proportional-integral-derivative controller. The highest stability obtained is 2.6x 10<sup>-11</sup> (π = 10 s), which is an improvement of 2 to 3 orders of magnitude over the free-running state. In this method, the concurrent intensity modulation is much smaller than in the current modulation method.
Thread-local heaps for Java (2002)

We present a memory management scheme for Java based on thread-local heaps. Assuming most objects are created and used by a single thread, it is desirable to free the memory manager from redundant synchronization for thread-local objects. Therefore, in our scheme each thread receives a partition of the heap in which it allocates its objects and in which it does local garbage collection without synchronization with other threads. We dynamically monitor to determine which objects are local and which are global. Furthermore, we suggest using profiling to identify allocation sites that almost exclusively allocate global objects, and allocate objects at these sites directly in a global area.We have implemented the thread-local heap memory manager and a preliminary mechanism for direct global allocation on an IBM prototype of JDK 1.3.0 for Windows. Our measurements of thread-local heaps with direct global allocation on a 4-way multiprocessor IBM Netfinity server show that the overall garbage collection times have been substantially reduced, and that most long pauses have been eliminated.
Constitutive and modulated expression of the human alpha 1 antitrypsin gene. Different transcriptional initiation sites used in three different cell types. (1992)

alpha 1-Antitrypsin (alpha 1 AT) is plasma glycoprotein that constitutes the principle inhibitor of neutrophil elastase in tissue fluids. It has been considered a prototype for liver-derived acute phase proteins in that its concentration in plasma increases three- to fourfold during the host response to inflammation/tissue injury. However, recent studies have shown that alpha 1 AT is expressed in several types of extrahepatic cells, including mononuclear phagocytes and enterocytes, and that there are distinct transcriptional units used in hepatocytes and at least one extra-hepatic cell type, blood monocytes. In this study, we have used a combination of ribonuclease protection assays, primer elongation analysis, and transcriptional run-on assays to further characterize mechanisms of basal and modulated alpha 1 AT gene expression in hepatocytes, enterocytes, and macrophages. The hepatoma cell line HepG2, intestinal epithelial cell line Caco2, and primary cultures of human peripheral blood monocytes were used as examples of the cell types. The results indicate that there are three macrophage-specific transcriptional initiation sites upstream from a single hepatocyte-specific transcriptional initiation site. Macrophages use these sites during basal and modulated expression. Hepatoma cells use the hepatocyte-specific transcriptional initiation site during basal and modulated expression but also switch on transcription from the upstream macrophage transcriptional initiation sites during modulation by the acute phase mediator interleukin 6 (IL-6). Caco2 cells use the hepatocyte-specific transcriptional initiation site during basal expression. There is a marked increase in the use of this site and an increase in the rate of transcriptional elongation of alpha 1 AT mRNA during differentiation of Caco2 cells from crypt-type to villous-type enterocytes. Caco2 cells also switch on transcription from the upstream macrophage transcriptional initiation sites during modulation by IL-6. These results provide further evidence that there are differences in the mechanisms of constitutive and regulated expression of the alpha 1 AT gene in at least three different cell types, HepG2-derived hepatocytes, Caco2-derived enterocytes and mononuclear phagocytes.
The effect of low earth orbit atomic oxygen exposure on phenylphosphine oxide‐containing poly(arylene ether)s (1998)

Unoriented thin films of phenylphosphine oxide-containing poly(arylene ether)s were exposed to low Earth orbit aboard the space shuttle Atlantis (STS-51) as part of a flight experiment designated Limited Duration Candidate Exposure (LDCE 4–5). The samples were exposed to primarily atomic oxygen (!10,\7×1019 atoms/cm2). Based on post-flight analyses using atomic force microscopy, X-ray photoelectron spectroscopy, gel permeation chromatogrpahy and weight loss data, it was proposed that atomic oxygen exposure of these materials produces a phosphate layer at the surface of the samples, apparently by the reaction of atomic oxygen with the phosphorus in the polymer backbone. Ground-based oxygen plasma exposure experiments have previously shown that this phosphate layer provides a barrier against further attack by atomic oxygen [1]. The results obtained from these analyses compare favorably with those obtained from samples exposed to an oxygen plasma in ground-based exposure experiments [1]. © 1998 John Wiley & Sons, Ltd.
Translational nonequilibrium in free jet expansions (1974)

The velocity distribution function for helium in translational nonequilibrium in hypersonic free jet expansions has been investigated with an electron beam fluorescence technique. A unique computer controlled Fabry-Perot interferometer and data acquisition system has been used to observe the 5015.67 Å radiation from the 31 P-21 S transition in helium. From the spectral structure, the Doppler broadening and shift of the line due to the velocity distribution function can be determined. With this technique parallel and perpendicular gas kinetic temperatures, mean flow velocity, and relative density measurements were made along the centerline of the free jet, from near equilibrium conditions through the midpoint of transition (T‖/T⊥ = 2.0). A comparison with kinetic theory predictions of the temperatures and velocity distribution functions indicate that the adoption of the ellipsoidal model, although sufficient for fitting the measured distribution function, is inadequate for determining the parallel temperature. The experimental data have been compared with a Navier-Stokes theory, with moment solutions of the Boltzmann equation, and a rarefication parameter suggested from previous Monte Carlo calculations. Determination of an effective hardsphere diameter from the experimental data is also presented.
Recombinant MVA-prime elicits neutralizing antibody responses by inducing antigen-specific B cells in the germinal center (2021)

The RV144 HIV-1 vaccine trial has been the only clinical trial to date that has shown any degree of efficacy and associated with the presence of vaccine-elicited HIV-1 envelope-specific binding antibody and CD4+ T-cell responses. This trial also showed that a vector-prime protein boost combined vaccine strategy was better than when used alone. Here we have studied three different priming vectors-plasmid DNA, recombinant MVA, and recombinant VSV, all encoding clade C transmitted/founder Env 1086 C gp140, for priming three groups of six non-human primates each, followed by a protein boost with adjuvanted 1086 C gp120 protein. Our data showed that MVA-priming favors the development of higher antibody binding titers and neutralizing activity compared with other vectors. Analyses of the draining lymph nodes revealed that MVA-prime induced increased germinal center reactivity characterized by higher frequencies of germinal center (PNA<sup>hi</sup>) B cells, higher frequencies of antigen-specific B-cell responses as well as an increased frequency of the highly differentiated (ICOS<sup>hi</sup>CD150<sup>lo</sup>) Tfh-cell subset.
Chiroptical Sensing of Asymmetric Hydrocarbons Using a Homochiral Supramolecular Box from a Bismetalloporphyrin Rotamer (2007)

Die Form folgt der Konfiguration: Das Zinkporphyrindimer 1 mit Pyridylgruppen kann einen asymmetrischen Kohlenwasserstoff wie Limonen chiroptisch nachweisen, indem es das homochirale schachtelförmige tetramere Assoziat BOX⊥ bildet. Da das gebildete BOX⊥ enantiomerenangereichert ist, können die optische Reinheit wie auch die absolute Konfiguration des Solvens Limonen bestimmt werden. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2001/2007/z604330_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
The miR-200c/141-ZEB2-TGFβ axis is aberrant in human T-cell prolymphocytic leukemia (2021)

T-cell prolymphocytic leukemia (T-PLL) is mostly characterized by aberrant expansion of small- to medium-sized prolymphocytes with a mature post-thymic phenotype, high aggressiveness of the disease and poor prognosis. However, T-PLL is more heterogeneous with a wide range of clinical, morphological, and molecular features, which occasionally impedes the diagnosis. We hypothesized that T-PLL consists of phenotypic and/or genotypic subgroups that may explain the heterogeneity of the disease. Multi-dimensional immuno-phenotyping and gene expression profiling did not reveal clear T-PLL subgroups, and no clear T-cell receptor a or β CDR3 skewing was observed between different T-PLL cases. We revealed that the expression of microRNA (miRNA) is aberrant and often heterogeneous in T-PLL. We identified 35 miRNA that were aberrantly expressed in T-PLL with miR-200c/141 as the most differentially expressed cluster. High miR- 200c/141 and miR-181a/181b expression was significantly correlated with increased white blood cell counts and poor survival. Furthermore, we found that overexpression of miR-200c/141 correlated with downregulation of their targets ZEB2 and TGFβR3 and aberrant TGFβ1- induced phosphorylated SMAD2 (p-SMAD2) and p-SMAD3, indicating that the TGFβ pathway is affected in T-PLL. Our results thus highlight the potential role for aberrantly expressed oncogenic miRNA in T-PLL and pave the way for new therapeutic targets in this disease.
Logical minimization of multilevel coded functions (1986)

Discrete numerical values in digital processing systems may be encoded in two-level (binary) or higher-level (multilevel) representations. Multilevel coding can produce smaller and more efficient processors. In truth-table lookup processing, the number of entries (reference patterns) can be reduced using multilevel coding. Since parallel-input/parallel-output optical truth-table lookup processors can be constructed based on holographic content-addressable memories, it is essential to know the minimum storage required to implement various functions. A new simple method for reducing multivalued functions is presented. This method is based on an extension of the Quine-McCluskey minimization method used for binary logic functions. This minimization method is then applied to the truth tables representing (1) modified signed-digit addition, (2) residue addition, and (3) residue multiplication. A programmable logic array gate configuration for the modified signed-digit adder is presented.
Apolipoprotein(a) inhibits hepatitis C virus entry through interaction with infectious particles (2017)

Our results identify apo(a) as an additional component of the lipid metabolism modulating HCV infection. (Hepatology 2017;65:1851-1864).
Evaluation d'une nouvelle approche vaccinale contre la bluetongue ciblant un antigène de groupe (2009)

National audience
Evanescent wave photonic crystal fiber tunable filter using dispersive optical polymers (2005)

We demonstrate wideband tunable photonic crystal fiber filters based on dispersive evanescent wave tunneling. The wavelength tuning range is /spl sim/400 nm by 10/spl deg/C temperature variation and the extinction ratio of power is above 45 dB.
3.1 A 25Gb/s ADC-based serial line receiver in 32nm CMOS SOI (2016)

As CMOS devices continue to scale down in voltage and area, digital-based high-speed serial I/Os [1] become increasingly competitive with analog-based designs [2,3]. In addition to offering the PVT-independent performance of digital functions and superior power and area scaling to future technology nodes, digital-based I/Os can support advanced line modulation techniques that will become necessary as long-reach electrical channel data rates scale to 56Gb/s and beyond. The key enablers of a digital receiver are power and area efficient analog to digital conversion (ADC) and digital channel equalization. This paper describes the design of a 25Gb/s 2-level digital serial line receiver including a ¼-rate 5b flash ADC, an 8-tap feed-forward equalizer (FFE), an 8-tap decision-feedback equalizer (DFE), and a baud-rate clock and data recovery circuit (CDR). The receiver features a flash ADC, which employs a new power and area efficient slicer design capable of achieving high-precision (∼1mV) threshold accuracy with an associated on-chip calibration system. The 32nm SOI CMOS receiver achieves error-free operation with margin on a reflective transmission-line channel with 40dB half-baud loss.
Editorial Commentary (2015)

No AccessUrology PracticePatient Care1 May 2015Editorial Commentary Massimiliano Spaliviero and James A. Eastham Massimiliano SpalivieroMassimiliano Spaliviero and James A. EasthamJames A. Eastham View All Author Informationhttps://doi.org/10.1016/j.urpr.2014.10.014AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "Editorial Commentary." Urology Practice, 2(3), p. 131 References 1 : Salvage therapies after radiation therapy. Urol Oncol2012; 30: 940. Google Scholar © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 2Issue 3May 2015Page: 131 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Massimiliano Spaliviero More articles by this author James A. Eastham More articles by this author Expand All Advertisement PDF downloadLoading ...
EXPLORATORY PHOTOCHEMISTRY OF 5‐AZIDO‐8‐ALKOXY–SUBSTITUTED PSORALENS FREE AND BOUND TO DNA (1995)

5-Azido-8-alkoxy psoralens were synthesized. Laser flash photolysis (LFP: XeF, 351 nm, 55 mJ, 17 ns) of the azides in acetonitrile or benzene solution produces the triplet nitrene and a small amount of ketenimine. Laser flash photolysis of the azides in methanol or aqueous solution cleanly produces the triplet nitrene. In aqueous solution containing highly polymerized calf thymus DNA, LFP produces a mixture of triplet nitrene and ketenimine corresponding to photolysis of free and bound psoralen, respectively. The two transients decay slowly but at different rates. Assignment of the transient spectra were secured by matrix EPR and UV-visible spectroscopy. The triplet nitrene lifetime is the same in buffer and in the presence and absence of calf thymus DNA. The results explain why psoralen azides are unable to efficiently nick plasmid DNA pBR322 upon UV activation.
High-output microwave detector using voltage-induced ferromagnetic resonance (2014)

We investigated the voltage-induced ferromagnetic resonance (FMR) with various DC bias voltage and input RF power in magnetic tunnel junctions. We found that the DC bias monotonically increases the homodyne detection voltage due to the nonlinear FMR originating in an asymmetric magnetization-potential in the free layer. In addition, the linear increase of an output voltage to the input RF power in the voltage-induced FMR is more robust than that in spin-torque FMR. These characteristics enable us to obtain an output voltage more than ten times than that of microwave detectors using spin-transfer torque.
Development of boundary element method to analyze acoustic models with comparison to finite element model (2015)

Numerical and computational models provide valuable information about engineering designs. Of their desirable benefits is reduction of expenses and feasibility of observing the change in behavior of a model with varying design parameters. In this paper, a boundary element method that was developed in Matlab is described. The approach in developing this method is based on the Green’s function for an unbounded acoustical domain. This model allows reading of computer aided designs to further analyze them in acoustic domain. The results obtained from this method are compared with equivalent finite element models carried out in ANSYS and exact analytical solutions to check for accuracy. Steps in analyzing acoustic models in ANSYS and Matlab are also explained in this paper.
Sperm Granulomas: An Adverse Effect of Vasectomy (1973)

No AccessJournal of Urology1 Oct 1973Sperm Granulomas: An Adverse Effect of Vasectomy Arnold M. Kwart, and Donald S. Coffey Arnold M. KwartArnold M. Kwart More articles by this author , and Donald S. CoffeyDonald S. Coffey More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)60238-6AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail © 1973 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited ByMarshall F, Ewing L, Zirkin B and Cochran R (2018) Testicular Atrophy Associated with Agenesis of the Epididymis in the ACI RatJournal of Urology, VOL. 127, NO. 1, (155-158), Online publication date: 1-Jan-1982.Mcloughlin M (2018) Clamp For Microsurgical VasovasostomyJournal of Urology, VOL. 118, NO. 1 Part 1, (69-69), Online publication date: 1-Jul-1977.Hagan K and Coffey D (2018) The Adverse Effects of Sperm During VasovasostomyJournal of Urology, VOL. 118, NO. 2, (269-273), Online publication date: 1-Aug-1977.Brannen G, Eggleston J, Adams J and Coffey D (2018) The Effects of Immunosuppression on Sperm Granulomas in Vasectomized RatsJournal of Urology, VOL. 112, NO. 6, (793-796), Online publication date: 1-Dec-1974. Volume 110Issue 4October 1973Page: 416-422 Advertisement Copyright & Permissions© 1973 by The American Urological Association Education and Research, Inc.MetricsAuthor Information Arnold M. Kwart More articles by this author Donald S. Coffey More articles by this author Expand All Advertisement Loading ...