gebhart/patrepeval huggingface dataset:
Targeting Orai1‐mediated store‐operated Ca2+ entry by a novel compound RP4010 for anti‐proliferative activity against esophagus squamous cell carcinoma (2018)

Targeting Orai1‐mediated store‐operated Ca2+ entry by a novel compound RP4010 for anti‐proliferative activity against esophagus squamous cell carcinoma The University of Texas System STARs Award Esophageal cancer is the 6th leading cause of cancer mortality worldwide with poor prognosis and 5‐year survival rate as low as 18%. It is an unmet need to develop more effective chemotherapeutic drugs for esophageal cancer patients. We previously reported that the expression of Orai1, a Ca2+ channel, was elevated in esophageal squamous cell carcinoma (ESCC), a major form of esophageal cancer, and the high expression is associated with poor survival rate in patients. Orai1‐mediated store‐operated calcium entry (SOCE) has been demonstrated to contribute to cancer cell proliferation, migration and invasion in ESCC and other cancers. Here we evaluated the anti‐cancer effect of a novel Orai1 channel inhibitor, named RP4010, in cultured ESCC cells and xenograft animals. RP4010 is a novel, oral inhibitor of Orai1 channel currently in Phase I/IB clinical development. Compared to commonly used Orai1 channel blockers (such as BTP‐2), RP4010 is more potent to block Orai1‐mediated SOCE and cell proliferation in ESCC cells. The IC50 of RP4010 in cell viability analysis were calculated as 1.2, 1.4, 1.4 and 5.5 μM for KYSE‐30, KYSE‐70, KYSE‐150 and KYSE‐790, respectively while IC50 for BTP‐2 was 17.7 – 17.8 μM for these cells. Treatment of RP4010 (10 μM) inhibited intracellular Ca2+ oscillations, halted Ca2+‐dependent nuclear translocation of NF‐κB, and resulted in cell cycles arrested at G0/G1 phase. Interestingly, RP4010 did not induce significant cell death even at rather high concentrations. In vivo study revealed that the mice can tolerate daily I.P. injection of 20 mg/kg RP4010 without body weight loss or noticeable adverse effects up to 4 weeks. At this dose, RP4010 could significantly reduce tumor growth compared to vehicle treated control group. In summary, these data demonstrated that R4010 is a novel potent inhibitor for Orai1‐meidated SOCE. It has potential as a new kind of targeted compound blocking Orai1‐mediated intracellular Ca2+ signaling in ESCC treatment. Support or Funding Information National Cancer Institute and The University of Texas System STARs Award This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .
Web content accessibility guidelines 1.0 (2001)

article Share on Web content accessibility guidelines 1.0 Authors: Wendy Chisholm Trace R & D Center, Univ. of Wisconsin—Madison Trace R & D Center, Univ. of Wisconsin—MadisonView Profile , Gregg Vanderheiden Trace R & D Center, Univ. of Wisconsin—Madison Trace R & D Center, Univ. of Wisconsin—MadisonView Profile , Ian Jacobs W3C W3CView Profile Authors Info & Claims InteractionsVolume 8Issue 4July 2001 pp 35–54https://doi.org/10.1145/379537.379550Published:01 July 2001Publication History 76citation4,209DownloadsMetricsTotal Citations76Total Downloads4,209Last 12 Months88Last 6 weeks13 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my Alerts New Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
Double-stranded gap repair in the photosynthetic prokaryote Synechococcus R2 (1986)

The photosynthetic cyanobacterium Synechococcus R2 is transformed by chimeric donor molecules lacking a functional replication origin but containing a region of homology to the recipient chromosome. These integrating donor molecules consist of a fragment of Synechococcus R2 chromosomal DNA cloned in the Escherichia coli vector pBR322 and interrupted by a piece of foreign DNA. During integration, this interrupting DNA is often lost by nonreciprocal exchange between homologous regions of donor and recipient. When transformed with donor molecules containing in vitro-generated double-stranded gaps or deletions as large as 20 kilobase pairs in the fragment homologous to the recipient chromosome, Synechococcus R2 can repair these lesions by using recipient information. Chromosomal DNA of the resulting transformants contains direct repeats of the recipient copy on either side of integrated pBR322 DNA. Homologous recombination between these repeats generates a circular molecule that can be recovered by transformation to E. coli. Plasmids recovered in E. coli contain the entire copy of information initially present in the region of the Synechococcus recipient corresponding to the donor gap or deletion. We suggest applications of this mechanism for cloning of genes identified by transposon mutagenesis.
Reconciling Gene Expression Data With Known Genome-Scale Regulatory Network Structures (2003)

The availability of genome-scale gene expression data sets has initiated the development of methods that use this data to infer transcriptional regulatory networks. Alternatively, such regulatory network structures can be reconstructed based on annotated genome information, well-curated databases, and primary research literature. As a first step toward reconciling the two approaches, we examine the consistency between known genome-wide regulatory network structures and extensive gene expression data collections in Escherichia coli and Saccharomyces cerevisiae. By decomposing the regulatory network into a set of basic network elements, we can compute the local consistency of each instance of a particular type of network element. We find that the consistency of network elements is influenced by both structural features of the network such as the number of regulators acting on a target gene and by the functional classes of the genes involved in a particular element. Taken together, the approach presented allows us to define regulatory network subcomponents with a high degree of consistency between the network structure and gene expression data. The results suggest that targeted gene expression profiling data can be used to refine and expand particular subcomponents of known regulatory networks that are sufficiently decoupled from the rest of the network.
A two‐dose epidural morphine regimen in cesarean section patients: pharmacokinetic profile (1993)

The maternal pharmacokinetics, metabolism of, and possible neonatal transmission of epidural morphine in cesarean section patients were investigated. Maternal plasma, breast milk, and maternal and neonatal urine concentrations of unconjugated and conjugated (UM and CM) morphine were measured in patients given two 5-mg doses of epidural morphine for post-cesarean section analgesia. The first dose was administered after delivery and the second dose 24 h later. Maternal venous blood samples (n = 10) were collected at times 0, 0.25, 0.5, 1, 2, 3, 4, 6, 12, and 24 h after each dose, and maternal urine was collected for three consecutive 24-h periods (n = 30). Maternal breast milk (n = 30), and neonatal urine samples (n = 20) were also collected. Serum, urine, and breast milk UM and CM levels were measured using radioimmunoassay. Pharmacokinetic values were calculated using noncompartmental analysis. The results were expressed as mean +/- 1 s.e. mean and analyzed using repeated measures analysis of variance and the paired t-test. Maternal serum UM remained 40-50% higher, and CM 50-100% higher in the first hour following dose 2 than the respective values after dose 1 (P < 0.05). Values for AUC, AUMC, T1/2, and MRT increased 28%, 83%, 35% and 36%, respectively, with the second dose (P < 0.05), while CI decreased 19% (P < 0.05) with no significant difference in Vss. Total urinary excretion of morphine decreased significantly from 1.98 +/- 0.15 mg on day 1 to 1.6 +/- 0.2 mg and 0.19 +/- 0.002 mg on days 2 and 3, respectively (P < 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
Analytic representations of the dielectric functions of crystalline and amorphous Si and crystalline Ge for very large scale integrated device and structural modeling (1998)

Various representations of the dielectric functions of semiconducting materials have been proposed. We compare five different models for representing the dielectric functions of crystalline (c-) Si, amorphous (a-) Si, and c-Ge from 1.5 to 6.0 eV. The four-oscillator critical point with parity plus one-dimensional tight-binding model (CPP-1D) best represents c-Si. Different models best represent a-Si and c-Ge. These representations provided a basis for modeling variations encountered in semiconductor manufacturing.
Towards Preserving Server-Side Privacy of On-Device Models (2022)

Machine learning-based predictions are popular in many applications including healthcare, recommender systems and finance. More recently, the development of low-end edge hardware (e.g., Apple’s Neural Engine and Intel’s Movidius VPU) has provided a path for the proliferation of machine learning on the edge with on-device modeling. Modeling on the device reduces latency and helps maintain the user’s privacy. However, on-device modeling can leak private server-side information. In this work, we investigate on-device machine learning models that are used to provide a service and propose novel privacy attacks that can leak sensitive proprietary information of the service provider. We demonstrate that different adversaries can easily exploit such models to maximize their profit and accomplish content theft. Motivated by the need to preserve both client and server privacy, we present preliminary ideas on thwarting such attacks.
Supplementary Table 3 from Gene Expression Profiling using Nanostring Digital RNA Counting to Identify Potential Target Antigens for Melanoma Immunotherapy (2023)

&lt;p&gt;XLSX file - 93K, Supplemental Table S3. TUMORS: 1 2 3 4 5 6 MELANOMA RELATED GENES.&lt;/p&gt;
Seeding Mechanism for High-Pressure Nozzles (2021)

View Video Presentation: https://doi.org/10.2514/6.2021-1068.vid NASA tests new launch and reentry vehicle configurations in wind tunnels, where flow visualizations and quantitative flowfield measurements are often desired. Some of these vehicles have rocket motors for propulsion, retro-propulsion, or reaction control. High-pressure air is used to supply these rocket motor plumes. However, it is difficult to make off-body measurements in these regions, for several reasons. First, the plumes themselves lack seeding particles for flow diagnostics, and at the low pressures after expansion, Rayleigh scattering or other molecular techniques yield insufficient signal for flow velocity measurements. Second, the plumes displace particle-seeded tunnel air, preventing measurements in the vicinity of the plume. Third, the plumes force shock waves ahead of the vehicle, which melts the ice crystal fog commonly used for visualization and measurement techniques in certain facilities. In the current work, a novel method for seeding the flow in these small-scale, high-pressure nozzles has been devised and initially demonstrated, potentially enabling quantitative and qualitative measurements with particle-based instruments such as Doppler global velocimetry or particle image velocimetry. The method involves a Venturi contraction to draw the seed liquid out of a reservoir and into the nozzle channel, wherein shearing forces atomize the seed into particles. The concept was tested with a laser sheet visualization, which demonstrated that the flow rate of liquid spray was controllable; a valve could be adjusted to drop the flow rate by up to 65%. This relatively inexpensive and simple technique may prove useful in wind tunnel experiments involving particle-based laser diagnostics and small, high-pressure nozzles.
Improved thermal stability of AlGaAs–GaAs quantum well heterostructures using a ‘‘blocking’’ Zn diffusion to reduce column-III vacancies (1995)

Data are presented on the reduction of layer intermixing (disordering) in AlGaAs–GaAs quantum well heterostructures (QWH) during high-temperature anneals by an initial low-temperature ‘‘blocking’’ Zn diffusion. Room-temperature photoluminescence measurements of the increase in the lowest electron-to-heavy-hole transition energy in the QW are used to characterize the extent of layer intermixing. Doped (C and Si) samples annealed (850 °C, 12 h) after a low-temperature blocking Zn diffusion (480 °C) exhibit reductions in energy shift from ∼177 meV to as little as ∼18 meV. Similar effects are also observed, but to a lesser extent, for undoped samples. The improved thermal stability is attributed to a Zn-diffusion induced reduction in the number of column-III vacancies in the active layers and is confirmed by secondary-ion mass spectroscopy measurements.
Application of the small-angle approximation to ocean water types (2001)

The small-angle approximation to the radiative transport equation is applied to particle suspensions that emulate ocean water. A particle size distribution is constructed from polystyrene and glass spheres with the best available data for particle size distributions in the ocean. A volume scattering function is calculated from the Mie theory for the particles in water and in oil. The refractive-index ratios of particles in water and particles in oil are 1.19 and 1.01, respectively. The ratio 1.19 is comparable to minerals and nonliving diatoms in ocean water, and the ratio 1.01 is comparable to the lower limit for microbes in water. The point-spread functions are measured as a function of optical thickness for both water and oil mixtures and compared with the point-spread functions generated from the small-angle approximation. Our results show that, under conditions that emulate ocean water, the small-angle approximation is valid only for small optical thicknesses. Specifically, the approximation is valid only for optical thicknesses less than 3.
Abstract 106: Characterizing Aortic Wall Dynamics in Murine Models of Abdominal Aortic Aneurysms Using Ultrasound Imaging (2016)

The objective of this study is to use non-invasive ultrasound imaging to characterize aortic wall dynamics in abdominal aortic aneurysms (AAAs). We induced AAAs (n=5) in apolipoprotein-E deficient mice by subcutaneously implanting an osmotic pump, which systemically delivers angiotensin II at a rate of 1000 ng/kg/min for 28 days, in the back of each mouse. Once aneurysm formations were confirmed using a high frequency small animal ultrasound system (Vevo2100, Visualsonics; 40 MHz central frequency), we implemented our cardiac- and respiratory-gated volumetric ultrasound technique to acquire 3D AAA geometries throughout a cardiac cycle. Figure 1 summarizes the results obtained from a representative mouse with a suprarenal AAA. A 3D volume rendering of an abdominal aorta, with the aneurysm positioned in the center, is illustrated next to a color bar, which provides position references for both the maximum circumferential Green-Lagrangian (GL) strain and the instantaneous strain plots. Qualitative inspections of both strain plots depicted in Figure 1 demonstrate that maximum GL circumferential strain occurs during systole and is smallest within the AAA. Quantitative assessments of GL circumferential strain for all five AAAs were performed with similar results indicating that maximum GL strain decreased significantly within the aneurysm (1.1±0.2%) compared to proximal (9.3±5.3%) and distal (4.0±2.1%) regions (p&lt;0.05). This decrease in GL circumferential strain within the aneurysmal portion of the abdominal aorta corroborates the idea that an increase in vessel stiffness is associated with the degradation of elastin and increased collagen turnover in the AAA wall. Interestingly, in all five cases, we observed a stiffer distal region, when compared to regions proximal to the aneurysm, suggesting that AAA development may also contribute to extracellular matrix remodeling of the aortic wall downstream of the aneurysm site.
Excited electronic states of ethylene sulfide. Circular dichroism study of (+)-(R,R)-2,3-dimethylthiirane (1983)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTExcited electronic states of ethylene sulfide. Circular dichroism study of (+)-(R,R)-2,3-dimethylthiiraneD. Cohen, M. Levi, B. S. Green, R. Arad-Yellin, Harold Basch, and A. GedankenCite this: J. Phys. Chem. 1983, 87, 23, 4585–4588Publication Date (Print):November 1, 1983Publication History Published online1 May 2002Published inissue 1 November 1983https://pubs.acs.org/doi/10.1021/j100246a010https://doi.org/10.1021/j100246a010research-articleACS PublicationsRequest reuse permissionsArticle Views22Altmetric-Citations7LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Outcomes of Surgical Treatment in Male Versus Female Adolescent Idiopathic Scoliosis Patients (2007)

Male AIS patients had slightly more rigid primary curves compared to females but a similar degree of postoperative scoliosis correction. Differences in the preoperative status and perioperative course did not compromise the outcomes of surgical treatment as in all other measures; the results were comparable between the genders.
The type II O‐antigenic polysaccharide moiety of Burkholderia pseudomallei lipopolysaccharide is required for serum resistance and virulence (1998)

Melioidosis, an infection caused by the gram-negative bacterial pathogen Burkholderia pseudomallei, is endemic in south-east Asia and northern Australia. Acute septicaemic melioidosis is a major cause of morbidity and mortality, especially in north-east Thailand. B. pseudomallei is highly resistant to the bactericidal activity of normal human serum (NHS), and we have found that B. pseudomallei 1026b multiplies in 10-30% NHS. We developed a simple screen for the identification of serum-sensitive mutants based on this novel phenotype. Approximately 1200 Tn5-OT182 mutants were screened, and three serum-sensitive mutants were identified. The type II O-antigenic polysaccharide (O-PS) moiety of lipopolysaccharide was not present in the serum-sensitive mutants. A representative serum-sensitive mutant, SRM117, was killed by the alternative pathway of complement and was less virulent than 1026b in three animal models of melioidosis. The Tn5-OT182 integrations in the serum-sensitive mutants were physically linked on the B. pseudomallei chromosome, and further genetic analysis of this locus revealed a cluster of 15 genes required for type II O-PS production. The proteins encoded by these genes were similar to proteins involved in bacterial polysaccharide biosynthesis. The results presented here demonstrate that type II O-PS is essential for B. pseudomallei serum resistance and virulence.
Infectious disease surveillance during emergency relief to Bhutanese refugees in Nepal (1994)

Simple, sustainable disease surveillance in refugee populations is essential during emergency relief efforts. Data can be used to direct community-based public health interventions to control common infectious diseases and reduce high mortality rates among refugees while placing a minimal burden on health workers.
Optimal Design Layout of a Tactical GSM Communication System with Nonuniform Traffic (2006)

An algorithm suitable for tactical TDMA-GSM communication system design is developed. The algorithm computes the minimum number of base stations for a given geography and provides for maximum coverage in the presence of nonuniform offered traffic. Optimal antenna location and sectorization are used to achieve the target coverage. The algorithm produces two distinct cell shapes (hexagonal and square) as the topography and propagation environment are changed. The cell size adapts to the traffic pattern to ensure the required quality of service, specifically a maximum blocking probability. As compared to other published work, this layout algorithm provides a comprehensive approach that takes into account both the nonuniform offered traffic and the area's varied topography. Several realistic system specifications were considered to simulate the minimum cost system resulting in a 20% savings in the required number of base stations compared to traditional design methods
Measurement of dijet azimuthal decorrelations in pp collisions at s=8  TeV with the ATLAS detector and determination of the strong coupling (2018)

Measurement of dijet azimuthal decorrelations in pp collisions at ffiffi s p = 8 TeV with the ATLAS detector and determination of the strong coupling
Sphingosine 1-Phosphate Induces Differentiation of Mesoangioblasts towards Smooth Muscle. A Role for GATA6 (2011)

Different cells can contribute to repair following vascular injury by differentiating into smooth muscle (SM) cells; however the extracellular signals involved are presently poorly characterized. Mesoangioblasts are progenitor cells capable of differentiating into various mesoderm cell types including SM cells. In this study the biological action exerted by the pleiotropic sphingolipid sphingosine 1-phosphate (S1P) in human mesoangioblasts has been initially investigated by cDNA microarray analysis. Obtained data confirmed the anti-apoptotic action of this sphingolipid and identified for the first time a strong differentiating action toward SM cells. Quantitative mRNA and protein analysis corroborated the microarray results demonstrating enhanced expression of myogenic marker proteins and regulation of the expression of transcription factor GATA6 and its co-regulator, LMCD1. Importantly, GATA6 up-regulation induced by S1P was responsible for the enhanced expression of SM-specific contractile proteins. Moreover, by specific gene silencing experiments GATA6 was critical in the pro-differentiating activity of the cytokine TGFβ. Finally, the pharmacological inhibition of endogenous S1P formation in response to TGFβ abrogated GATA6 up-regulation, supporting the view that the S1P pathway plays a physiological role in mediating the pro-myogenic effect of TGFβ. This study individuates GATA6 as novel player in the complex transcriptional regulation of mesoangioblast differentiation into SM cells and highlights a role for S1P to favour vascular regeneration.
Echocardiographic Features Defining Right Dominant Unbalanced Atrioventricular Septal Defect (2013)

The angle of right ventricle/LV inflow and other surrogates of inflow may be important defining echocardiographic measures of right dominant unbalanced AVSD, although confirmation is needed.
Coherent electron-phonon couplings in a charge density wave material (2020)

Using trARPES, we observe that the CDW amplitude mode in 1 T -TaSe2 can coherently modulate the electron temperature. Coherent electron-phonon coupling mechanisms are proposed to explain the electron temperature oscillations at different pump fluences.