gebhart/patrepeval huggingface dataset:
Recombinant immunotoxins and other therapies for relapsed/refractory hairy cell leukemia (2011)

Standard treatment for hairy cell leukemia (HCL) is markedly effective, but the constant decrease in disease-free survival, together with the presence of minimal residual disease (MRD), suggests that few if any are cured. HCL cells in MRD are always strongly CD20 + and CD22 + , and also CD25 + unless the patient has the poor-prognosis variant HCLv. To target relapsed/refractory HCL, immunotherapy has been developed using anti-CD25 and anti-CD22 recombinant immunotoxins, or the anti-CD20 monoclonal antibody (mAb) rituximab alone or combined with purine analogs. The recombinant immunotoxins contain an Fv fragment of a mAb fused to a truncated form of Pseudomonas exotoxin called PE38. BL22 targeting CD22, in phase I and II testing of relapsed/refractory HCL, achieved 47-61% complete remissions (CRs), several of them ongoing after 9-10 years. A completely reversible form of hemolytic uremic syndrome (HUS) was observed in 12% of patients, several of whom could later achieve a partial remission (PR) or CR with LMB-2 targeting CD25. A higher-affinity version of BL22, termed HA22, CAT-8015, or moxetumomab pasudotox, developed to more effectively treat other hematologic malignancies, also achieves CRs in HCL, and with only non-dose-limiting HUS. In separate randomized trials, rituximab is undergoing phase II testing with cladribine for early HCL and with bendamustine or pentostatin for multiply relapsed HCL.
A Study of Linear vs. Nonlinear Control Techniques for the Reconfiguration of Satellite Formations (2001)

This thesis investigates several linear and nonlinear feedback control methods for satellite formation reconfigurations and compares them to a near optimal open loop, discrete-time, impulsive maneuver. The reconfigurations are done in terms of a set of relative parameters that define an orbit about the leader satellite (or center reference position if a leader satellite does not exist at the center of the formation). The purpose of the study is two-fold, to compare the control usage of continuous feedback control methods versus a discrete bum method and to determine if nonlinear control techniques offer significant improvement over more conventional linear control laws. Linear Quadratic Regulators (LQR), LQR with linearizing feedback, State Dependent Riccati Equation (SDRE) and sliding mode controllers are considered. Simulations showed that reconfigurations for small relative orbits were adequately controlled using linear techniques.
Perineural antinociceptive effect of opioids in a rat model (2001)

The results indicate that perineural fentanyl or morphine do not produce analgesia. Perineural block produced by meperidine was attributed to local anesthetic-like effect, rather than to drug interaction with opioid receptor.
Lifetime-resolved photon-correlation Fourier spectroscopy (2021)

The excited state population of single solid-state emitters is subjected to energy fluctuations around the equilibrium driven by the bath and relaxation through the emission of phonons or photons. Simultaneous measurement of the associated spectral dynamics requires a technique with a high spectral and temporal resolution with an additionally high temporal dynamic range. We propose a pulsed excitation-laser analog of photon-correlation Fourier spectroscopy (PCFS), which extracts the linewidth and spectral diffusion dynamics along the emission lifetime trajectory of the emitter, effectively discriminating spectral dynamics from relaxation and bath fluctuations. This lifetime-resolved PCFS correlates photon-pairs at the output arm of a Michelson interferometer in both their time-delay between laser-excitation and photon-detection T and the time-delay between two photons τ. We propose the utility of the technique for systems with changing relative contributions to the emission from multiple states, for example, quantum emitters exhibiting phonon-mediated exchange between different fine-structure states.
Dependence of the electrical characteristics of organic-on-inorganic semiconductor contact barrier diodes on organic thin-film composition (1988)

Several aromatic compounds (such as 3,4,9,10 perylenetetracarboxylic dianhydride) have previously been reported to form rectifying heterojunction energy barriers when vacuum deposited onto inorganic semiconductor substrates such as Si, GaAs, and InP. In this paper we report the formation of rectifying heterojunction barriers using phthalocyanine-based compounds layered onto p-Si substrates. In many respects, the characteristics of phthalocyanine/Si heterojunctions are similar to those formed using the aromatic anhydrides insofar as the electrical properties can be explained using the thermionic emission space-charge-limited transport model introduced in previous work. However, in contrast to the earlier results, we find that a high density of surface states exist at the phthalocyanine/Si interface. Quantitative study of the interface state density provides insight into the mechanisms of formation of the organic-on-inorganic energy barrier.
Centrifugal Force-Driven Modular Micronozzle System: Generation of Engineered Alginate Microspheres (2019)

In this study, we developed a modular micronozzle system that can control the flow of fluid based on centrifugal force and synthesize functional alginate microspheres with various structures and sizes. Our method is to fabricate a programmable microreactor that can be easily manufactured without the conventional soft-lithography process using various sequences of the micronozzles with various inner diameters. To overcome the obstacles of pump-based microfluidic devices that need to be precisely controlled, we designed the programmable microreactor to be driven under centrifugal force with a combination of micronozzles, thus enabling the mass production of various functional alginate microspheres within a few minutes. The programmable microreactor designed through the arrangement of the modular micronozzles enables the formation of various types of alginate microspheres such as core-shell, Janus, and particle mixture. These materials are controlled to a size from 400 µm to 900 µm. In addition, our platform is used to generate pH-responsive smart materials, and to easily control various sizes, shapes, and compositions simultaneously. By evaluating the release process of model drugs according to the pH change, the possibility of drug delivery application is confirmed. We believe that our method can contribute to development of biomaterials engineering that has been limited by the requirement of sophisticated devices, and special skills and/or labor.
Behavioral Effects and Gene Delivery in a Rat Model of Parkinson's Disease (1995)

Article MetricsDownloadsCitationsNo data available.012AugSepOctNovDecJan240Total6 Months12 MonthsTotal number of downloads for the most recent 6 whole calendar months.
Selective Targeting of Protein Kinase C (PKC)-θ Nuclear Translocation Reduces Mesenchymal Gene Signatures and Reinvigorates Dysfunctional CD8+ T Cells in Immunotherapy-Resistant and Metastatic Cancers (2022)

Protein kinase C (PKC)-θ is a serine/threonine kinase with both cytoplasmic and nuclear functions. Nuclear chromatin-associated PKC-θ (nPKC-θ) is increasingly recognized to be pathogenic in cancer, whereas its cytoplasmic signaling is restricted to normal T-cell function. Here we show that nPKC-θ is enriched in circulating tumor cells (CTCs) in patients with triple-negative breast cancer (TNBC) brain metastases and immunotherapy-resistant metastatic melanoma and is associated with poor survival in immunotherapy-resistant disease. To target nPKC-θ, we designed a novel PKC-θ peptide inhibitor (nPKC-θi2) that selectively inhibits nPKC-θ nuclear translocation but not PKC-θ signaling in healthy T cells. Targeting nPKC-θ reduced mesenchymal cancer stem cell signatures in immunotherapy-resistant CTCs and TNBC xenografts. PKC-θ was also enriched in the nuclei of CD8<sup>+</sup> T cells isolated from stage IV immunotherapy-resistant metastatic cancer patients. We show for the first time that nPKC-θ complexes with ZEB1, a key repressive transcription factor in epithelial-to-mesenchymal transition (EMT), in immunotherapy-resistant dysfunctional PD1<sup>+</sup>/CD8<sup>+</sup> T cells. nPKC-θi2 inhibited the ZEB1/PKC-θ repressive complex to induce cytokine production in CD8<sup>+</sup> T cells isolated from patients with immunotherapy-resistant disease. These data establish for the first time that nPKC-θ mediates immunotherapy resistance via its activity in CTCs and dysfunctional CD8<sup>+</sup> T cells. Disrupting nPKC-θ but retaining its cytoplasmic function may offer a means to target metastases in combination with chemotherapy or immunotherapy.
Cellular kinetics of CTL019 in relapsed/refractory B-cell acute lymphoblastic leukemia and chronic lymphocytic leukemia (2017)

Tisagenlecleucel (CTL019) is an investigational immunotherapy that involves reprogramming a patient's own T cells with a transgene encoding a chimeric antigen receptor to identify and eliminate CD19-expressing cells. We previously reported that CTL019 achieved impressive clinical efficacy in patients with relapsed/refractory B-cell acute lymphoblastic leukemia (ALL) and chronic lymphocytic leukemia (CLL), including the expansion and persistence of CTL019 cells, which correlates with response to therapy. Here, we performed formal cellular kinetic analyses of CTL019 in a larger cohort of 103 patients treated with CTL019 in 2 different diseases (ALL and CLL). CTL019 was measured in peripheral blood and bone marrow, using quantitative polymerase chain reaction and flow cytometry. CTL019 levels in peripheral blood typically peaked at 10 to 14 days postinfusion and then declined slowly over time. Patients with complete response (CR)/CR with incomplete count recovery had higher levels of CTL019 in peripheral blood, with greater maximal concentration and area under the curve values compared with nonresponding patients (<i>P</i> < .0001 for each). CTL019 transgene levels were measurable up to 780 days in peripheral blood. CTL019 trafficking and persistence were observed in bone marrow and cerebrospinal fluid. CTL019 expansion correlated with severity of cytokine release syndrome (CRS) and preinfusion tumor burden in pediatric ALL. The results described here are the first detailed formal presentation of cellular kinetics across 2 diseases and highlight the importance of the application of in vivo cellular kinetic analyses to characterize clinical efficacy and CRS severity associated with CTL019 therapy.
Early transition metal nano-carbides and nano-hydrides from solid-state metathesis initiated at room temperature (2021)

Carbon supported nanoparticles of early transition metal (Zr, Nb, Mo, Ta, W) carbides and hydrides were synthesized from metal chlorides and potassium graphite without the need for extensive external heating.
Density-Gradient Control over Nanoparticle Supercrystal Formation* (2020)

With the advent of\nDNA-directed methods to form “single\ncrystal” nanoparticle superlattices, new opportunities for\nstudying the properties of such structures across many length scales\nnow exist. These structure–property relationships rely on the\nability of one to deliberately use DNA to control crystal symmetry,\nlattice parameter, and microscale crystal habit. Although DNA-programmed\ncolloidal crystals consistently form thermodynamically favored crystal\nhabits with a well-defined symmetry and lattice parameter based upon\nwell-established design rules, the sizes of such crystals often vary\nsubstantially. For many applications, especially those pertaining\nto optics, each crystal can represent a single device, and therefore\nsize variability can significantly reduce their scope of use. Consequently,\nwe developed a new method based upon the density difference between\ntwo layers of solvents to control nanoparticle superlattice formation\nand growth. In a top aqueous layer, the assembling particles form\na less viscous and less dense state, but once the particles assemble\ninto well-defined rhombic dodecahedral superlattices of a critical\nsize, they sediment into a higher density and higher viscosity sublayer\nthat does not contain particles (aqueous polysaccharide), thereby\narresting growth. As a proof-of-concept, this method was used to prepare\na uniform batch of Au nanoparticle (20.0 ± 1.6 nm in diameter)\nsuperlattices in the form of 0.95 ± 0.20 μm edge length\nrhombic dodecahedra with body-centered cubic crystal symmetries and\na 49 nm lattice parameter (cf. 1.04 ± 0.38 μm without the\nsublayer). This approach to controlling and arresting superlattice\ngrowth yields structures with a 3-fold enhancement in the polydispersity\nindex.
A 6-Month, Randomized, Double-Masked, Placebo-Controlled Study Evaluating the Effects of the Protein Kinase C-β Inhibitor Ruboxistaurin on Skin Microvascular Blood Flow and Other Measures of Diabetic Peripheral Neuropathy (2007)

In this cohort of DPN patients, ruboxistaurin enhanced SkBF at the distal calf, reduced sensory symptoms (NTSS-6), improved measures of Norfolk QOL-DN, and was well tolerated.
Association Between Use of Long-Acting Nitrates and Outcomes in Heart Failure With Preserved Ejection Fraction (2017)

In HFpEF, the use of nitrates was not associated with improvements in all-cause mortality or heart failure hospitalization.
Chemotaxis Towards Sugars by Bacillus subtilis (1979)

Many sugars and derivatives were tested in the capillary assay for their attraction of Bacillus subtilis. The major attractants were 2-deoxy-D-glucose, D-fructose, gentiobiose, D-glucose, maltose, D-mannitol, D-mannose, N-acetylglucosamine, alpha-methyl-D-glucoside, beta-methyl-D-glucoside, N-acetylmannosamine, alpha-methyl-D-mannoside, D-sorbitol, L-sorbose, sucrose, trehalose and D-xylose. Only glucose chemotaxis was completely constitutive. Competition experiments were carried out to determine the specificities of chemoreceptors. There were 25 instances of no influence of two sugars on each other's taxis, 92 instances of one sugar interfering non-reciprocally with chemotaxis towards another and 49 instances of two sugars reciprocally competing. However, in most of the last instances, other sugars were identified that interfered with chemotaxis towards one member of the pair but not the other. Thus, nearly all sugars and related compounds appear to be detected by their own chemoreceptors, but many secondary interactions exist.
Reaction Chemistry to Characterize the Molecular Initiating Event in Skin Sensitization: A Journey to Be Continued (2016)

The publication by the OECD of the adverse outcome pathway (AOP) for skin sensitization has accelerated the development and validation of mechanistic tests and testing strategies to assess the potential of new molecules to trigger skin allergies. The molecular initiating event (MIE) in the AOP is reaction with skin peptides/proteins. It is followed by a number of cellular events. Currently, only one in chemico test to characterize the MIE was proposed to and underwent adoption by the OECD, while two cell-based assays have completed the process. A multitude of further cellular assays is currently in the validation pipeline, but no further reactivity test has gone through full standardization. Here, we review data on in chemico methods, identify gaps, and discuss how these methods can be improved to better characterize the MIE and to become even more informative. We focus on the importance of kinetics, the information gained from studying adduct formation, and the difficulties posed by side reactions such as peptide oxidation. We then highlight mechanistic insights from reaction chemistry: the relative contribution of different target nucleophiles, the migration of amino acid modifications, and the potential of peptide-cross-linking. We illustrate in a case study how kinetic in chemico methods might have been used to better predict the risk of three preservatives, which have led to serious epidemics of contact dermatitis. In a case study on Michael acceptors, we show the impact of additional substituents around the electrophilic olefin moiety on reactivity, and we highlight the shortcomings which current in silico methods to predict reaction chemistry still have, illustrating the need for experimental in chemico data to improve such models. Finally, based on the information reviewed and the presented case studies, a strong argument is made to continue the journey of developing nonredundant, informative in chemico methods, and not to solely focus on new cell-based methods to further populate the AOP for skin sensitization.
Botulinum toxin type A for the treatment of lower urinary tract disorders (2012)

Many papers report the clinical success of botulinum toxin A as a method of management of various bladder dysfunctions. The rationale was that botulinum toxin A was able to block the presynaptic release of acetylcholine from the parasympathetic efferent nerve. The efficacy might result not only from an inhibitory effect on detrusor muscle, but also some effects might be mediated by altering the afferent nerve input. This systematic literature review discusses the efficacy and safety of botulinum toxin A therapy for idiopathic detrusor overactivity, neurogenic detrusor overactivity, interstitial cystitis/painful bladder syndrome and benign prostatic hyperplasia. The information was gathered from a PubMed literature research for abstracts from recent urological meetings. Injection of botulinum toxin A appears to have a positive therapeutic effect in multiple urological conditions, such as refractory idiopathic detrusor overactivity, neurogenic detrusor overactivity, interstitial cystitis/painful bladder syndrome and benign prostatic hyperplasia. Because the United States Food and Drug Administration has approved botulinum toxin A (Botox) for injection for the treatment of urinary incontinence as a result of neurogenic detrusor overactivity (e.g. spinal cord injury, multiple sclerosis) in adults who have an inadequate response to or are intolerant of an ant cholinergic medication, the use of botulinum toxin A will spread and be a more familiar therapy in the urological arena. However, further robust evidence should be awaited. We will discuss the current use of this agent within the urological field.
Photoelectrochemical Etching Technology for Gallium Nitride Power and RF Devices (2019)

Photoelectrochemical (PEC) etching was used to fabricate deep trench structures in GaN-on-GaN epilayers grown on n-GaN substrates. The width of the side etching was less than 1 mu m, with high accuracy. The aspect ratio (depth/width) of a 3.3-mu m-wide trench with a PEC etching depth of 24.3 mu m was 7.3. These results demonstrate the excellent potential of PEC etching for fabricating deep trenches in vertical GaN devices. Furthermore, we simplified the PEC etching technology to permit its use in a wafer-scale process. We also demonstrated simple contactless PEC etching technologies for the manufacture of power and RF devices. A trench structure was fabricated in a GaN-on-GaN epilayer by simple contactless PEC etching. The role of the cathodic reaction in contactless PEC etching is discussed in relation to the application of a GaN HEMT epilayer on a semi-insulating substrate. Fortunately, the GaN HEMT structure contains an ohmic electrode that can act as a cathode in contactless PEC etching, thereby permitting the recess etching of a GaN HEMT epilayer grown on a semi-insulating SiC substrate. These results indicate that PEC etching technologies are becoming suitable for use in the fabrication of practical GaN power and RF devices.
Oncothanin, a Peptide from the α 3 Chain of Type IV Collagen, Modifies Endothelial Cell Function and Inhibits Angiogenesis (2004)

Previous studies from our group and the group of the Department of Biochemistry at the University of Rheims, France [corrected] have shown that basement membrane (BM) collagen from anterior lens capsule type IV collagen (ALC-COL IV) and peptides from the noncollagenous domain (NC1) of the alpha3(IV) [corrected] chain, corresponding to residues 185-203 and 179-208, inhibit tumor cell proliferation, specifically through the interaction of the -SNS- tripeptide (residues 189-191) with the CD47/alphavbeta3 integrin receptor complex. Data presented here demonstrate that the alpha3(IV)185-203 and the alpha3(IV)179-208 peptides, from here forward [corrected] designated as oncothanin, regulate endothelial cell (EC) proliferation, adhesion, and motility which [corrected] ultimately influence angiogenesis. The data also indicate that oncothanin, when used as a chemoattractant, greatly enhanced EC chemotaxis. In contrast, pretreatment of EC with oncothanin inhibited chemotaxis toward several different chemoattractants. When oncothanin was used as a substrate, it enhanced EC adhesion that was inhibited when pretreated with same. Analysis of angiogenesis by EC differentiation (tube formation), aortic ring microvessel formation [corrected] and the chorioallantoic membrane assay, [corrected] demonstrate that oncothanin, but not the control medium or peptides, inhibits angiogenesis. In the EC differentiation assay, oncothanin completely inhibited tube formation at 25 microg/ml, whereas peptides with comparable sequences, that lacked [corrected] the -SNS- sequence, from ALC-COL IV NC1 domains alpha1 and alpha2 chains failed to inhibit tube formation. The data support the hypothesis that ALC-COL IV and oncothanin inhibit angiogenesis by modulation of EC function.
Ozone Removal at Micro-Second Contact Time for Aircraft Cabin Air Using Microfibrous Entrapped Catalysts (2012)

Microfibrous Entrapped Catalyst (MFEC) manufactured from 8 $m diameter nickel fibers were engineered into pleated heterogeneous catalytic reactors to improve removal performance over that of conventional packed bed and monolith reactors. The main enabling attribute of microfibrous materials is that small particles (150�200 $m) can be entrapped which significantly improves inter phase mass transfer. Conventional reactors usually operate with contact time less than 1s. MFEC reactors were able to reduce the contact time to micro seconds while maintaining similar catalytic performance. This gives MFEC a huge advantage in terms of weight and volume saving, since the conventional meterlong reactors were shortened to millimeter thick material sheets of MFEC. These unique reactors were targeted at ozone, which is widely recognized as the most important aircraft cabin air pollutant. In this research, MFEC reactors were investigated under aircraft bleed air conditions of high temperature (100�200 oC) and hig h face velocity (10�30 m/s) resulting in an interlayer contact time of 67�200$sec. Precious met al (Pd, Ag) and transition metal (Mn) catalysts were impregnated on entrapped particles (e.g. γ�Al2O3) using incipient wetness method. Ozone test concentration was set at a highdemand level of 1.5 ppmv. Results showed that a high level of ozone decomposition was achieved with a significant reduction of catalyst consumption. Compared with conventional aircraft filters, this can be a huge advantage in terms of material cost and labor. Reaction kinetics analyses were compared for different catalysts. Results showed that precious metal catalyst performs better at a higher temperature while transition metal catalyst maintains similar conversion for changing temperatures (100�200oC). XPS, TPR and TGA tests ha ve been used to evaluate performance of catalysts and to modify catalysts to improve conversion rate. Also, catalyst aging tests were performed according to the frequency of commercial aircraft ozone reactor replacement, which required evaluation of longterm performance for actual MFEC reactor. In addition, pressure drop improvement by pleating MFEC was also evaluated by comparison to traditional low pressure drop monolith reactors. A CFD model has also been established for pressure prediction and comparison. It showed that pleated structure enables