gebhart/patrepeval huggingface dataset:
Notch Signaling Activation in Cervical Cancer Cells Induces Cell Growth Arrest with the Involvement of the Nuclear Receptor NR4A2 (2016)

Cervical cancer is a second leading cancer death in women world-wide, with most cases in less developed countries. Notch signaling is highly conserved with its involvement in many cancers. In the present study, we established stable cervical cell lines with Notch activation and inactivation and found that Notch activation played a suppressive role in cervical cancer cells. Meanwhile, the transient overexpression of the active intracellular domain of all four Notch receptors (ICN1, 2, 3, and 4) also induced the suppression of cervical cancer Hela cell growth. ICN1 also induced cell cycle arrest at phase G1. Notch1 signaling activation affected the expression of serial genes, especially the genes associated with cAMP signaling, with an increase of genes like THBS1, VCL, p63, c-Myc and SCG2, a decrease of genes like NR4A2, PCK2 and BCL-2. Particularly, The nuclear receptor NR4A2 was observed to induce cell proliferation via MTT assay and reduce cell apoptosis via FACS assay. Furthermore, NR4A2's activation could reverse ICN1-induced suppression of cell growth while erasing ICN1-induced increase of tumor suppressor p63. These findings support that Notch signaling mediates cervical cancer cell growth suppression with the involvement of nuclear receptor NR4A2. Notably, Notch/NR4A2/p63 signaling cascade possibly is a new signling pathway undisclosed.
A 3D Human Liver Model of Nonalcoholic Steatohepatitis (2020)

<b><i>Background and Aims:</i></b> To better understand nonalcoholic steatohepatitis (NASH) disease progression and to evaluate drug targets and compound activity, we undertook the development of an <i>in vitro</i> 3D model to mimic liver architecture and the NASH environment. <b><i>Methods:</i></b> We have developed an <i>in vitro</i> preclinical 3D NASH model by coculturing primary human hepatocytes, human stellate cells, liver endothelial cells and Kupffer cells embedded in a hydrogel of rat collagen on a 96-well plate. A NASH-like environment was induced by addition of medium containing free fatty acids and tumor necrosis factor-α. This model was then characterized by biochemical, imaging and transcriptomics analyses. <b><i>Results:</i></b> We succeeded in defining suitable culture conditions to maintain the 3D coculture for up to 10 days <i>in vitro</i>, with the lowest level of steatosis and reproducible low level of inflammation and fibrosis. NASH disease was induced with a custom medium mimicking NASH features. The cell model exhibited the key NASH disease phenotypes of hepatocyte injury, steatosis, inflammation, and fibrosis. Hepatocyte injury was highlighted by a decrease of CYP3A4 expression and activity, without loss of viability up to day 10. Moreover, the model was able to stimulate a stable inflammatory and early fibrotic environment, with expression and secretion of several cytokines. A global gene expression analysis confirmed the NASH induction. <b><i>Conclusions:</i></b> This is a new <i>in vitro</i> model of NASH disease consisting of four human primary cell-types that exhibits most features of the disease. The 10-day cell viability and cost effectiveness of the model make it suitable for medium throughput drug screening and provide attractive avenues to better understand disease physiology and to identify and characterize new drug targets.
Effect of Tack Cure on Polymerization Shrinkage of Resin-based Luting Cements (2020)

Clinical Relevance Self-cure after tack cure could result in a lower polymerization shrinkage in some resin-based luting cements, which is closely related to lower degree of cure. SUMMARY Objectives: To evaluate the effect of tack cure on polymerization shrinkage (PS) of resin-based luting cements. Methods and Materials: One composite resin cement, Duo-Link (Duolink); two self-adhesive resin cements, RelyX U200 (U200) and G-CEM LinkAce (GCem); and one resin-modified glass ionomer cement, RelyX Luting Plus (Luting+), were used for measuring PS in light-cure (LC group), self-cure (SC group), and two tack-cure modes that were light cured (TC-LC group) or self-cured (TC-SC group) after tack cure. PS was measured by a modified bonded disc method for 1600 seconds and analyzed with two-way analysis of variance and Tukey honestly significant difference test. To investigate the effect of tack cure on light cure or self-cure, data were analyzed with an independent-samples t-test with tack cure as a variable. The significance level was 5%. Results: Regarding cure mode, Duolink showed a significantly lower PS in the TC-SC group compared with the other groups. Luting+ showed a significantly lower PS in the TC-SC group than in the SC group. U200 showed a significantly lower PS in the self-cure groups compared with that in the light-cure groups. The PS of GCem was not affected by cure mode. Regarding cements, Luting+ showed the highest PS, followed by GCem, Duolink, and U200 (p&amp;lt;0.05). Self-cure of Duolink and Luting+ was negatively affected by tack cure, while light cure was not affected. U200 and GCem were not affected by tack cure either in the self-cure or light-cure groups. Conclusion: For the tested cements, tack cure decreased the PS of Duolink and Luting+ when they were self-cured after tack cure. When the cements were light cured after tack cure, PS was not affected by tack cure in any cement.
Influence of the Ageing and Drying Steps of a CoMoP/γ‐Al2O3 Catalyst onto the Multi‐Scale Molybdenum Active Phase Organization (2023)

Abstract To study the influence of ageing and drying steps onto the multiscale Mo active phase arrangement ( i. e . MoS 2 slabs and slab aggregates), a series of highly loaded CoMoP/γ‐Al 2 O 3 catalysts aged, unaged, dried or freeze‐dried were prepared and tested in toluene hydrogenation. Electron Probe Micro Analyses (EPMA) and Anomalous Small Angle X‐Ray Scattering (ASAXS) permit to highlight the influence of each preparation steps. A growth of Mo entities occurs during ageing along with metal diffusion inside the support and leads to longer slabs. The same occurs during drying with metal redistribution due to solvent evaporation. Concomitantly, a fragmentation of slab aggregates takes place and leads to numerous small slabs aggregates. Slab length and aggregates amount were found to drive the catalytic activity, which translates the relationship between activity, number of active sites and their accessibility. Conversely, macroscopic metal distribution into the extrudates appears to have no impact on the catalytic performance.
Supplemental Figure S2 from Loss of <i>ARID1A</i> Activates <i>ANXA1</i>, which Serves as a Predictive Biomarker for Trastuzumab Resistance (2023)

&lt;p&gt;Supplemental Figure S2: Suppression of ARID1A leads to induction of ANXA1 RNA expression and loss of BRG1 complex binding to ANXA1 region&lt;/p&gt;
Can portable electronic devices (PEDs) interfere with aircraft systems? (2003)

Passengers requested to be allowed to work onboard of airplanes with PEDs of any kind. But this comfort is acceptable only if the airplanes are protected in such a way that all interferences of PEDs with aircraft systems, especially with flight critical systems are impossible. This paper report of PED measurements performed in the context of the European research programme EMHaz. The result is a new overall limit curve of PED emissions. Based on this limit curve, the threat of coupling into antennas and into installed cable looms for helicopters is investigated and the possible effects and functional influences on aircraft systems are deduced.
Microwave Accelerated Organic Transformations: Dibutylstannylene Acetal Mediated Selective Acylation of Polyols and Amino Alcohols using Catalytic Amounts of Dibutyltin Oxide. Influence of the Solvent and the Power Output on the Selectivity (1995)

The formation of dibutylstannylene acetals from polyols or amino alcohols and dibutyltin oxide is accelerated under microwave heating. We have found that under these conditions, only catalytic amounts of dibutyltin oxide (0.2 mol equiv.) are needed to achieve moderate to excellent conversions. The method is applied to the selective benzoylation of a variety of polyols and to an amino alcohol. The selectivity of the transformation can be modulated upon changes in some experimental variables (nature of the solvent and power output of the microwave oven).
Hydrogels from Polysaccharides for Biomedical Applications (2007)

Development of products made from renewable sources is considered to be a strategic research area by the international scientific community since it is generally accepted that the fossil fuels will be exhausted in the foreseeable future. Another related strategic area is the development of new bioactive and biocompatible polymers capable of exerting a temporary therapeutic function. Among other substances, polysaccharides have been proposed to be suitable materials as matrixes for the preparation of hydrogels, e.g., for use in key applications such as drug release systems and tissue engineering. Methods reported for preparation of hydrogels using renewable polysaccharides aimed for biomedical use will be reviewed.
Extensor Mechanism Allograft Reconstruction After Total Knee Arthroplasty (2005)

The results of reconstruction with an extensor mechanism allograft after total knee arthroplasty depend on the initial tensioning of the allograft. Loosely tensioned allografts result in a persistent extension lag and clinical failure. Allografts that are tightly tensioned in full extension can restore active knee extension and result in clinical success. On the basis of the number of knees that we studied, there was no significant loss of flexion. Use of an extensor mechanism graft for the treatment of a failure of the extensor mechanism will be successful only if the graft is initially tensioned tightly in full extension.
Molecular Exchange Kinetics of Diblock Copolymer Micelles Monitored by Fluorescence Correlation Spectroscopy (2014)

We investigated the equilibrium chain-exchange kinetics of amphiphilic diblock copolymer micelles, using a new method based on fluorescence correlation spectroscopy. The micelles were formed from polystyrene-<i>block</i>-poly[oligo(ethylene glycol) methyl ether methacrylate] (PS-POEGMA) in different solvents and studied at various temperatures. This linear-brush copolymer was chosen as a model system, forming micelles with short and bulky corona. Depending on the applied solvent, fast exchange could be observed even at temperatures well below the nominal glass transition of the core-forming PS block. The effect is caused by swelling of the core and allows extensive tuning of the chain-exchange rate by adding to the system minor amounts of good or bad solvent for the core block.
Air-depolyable geophysics package (1993)

We are using Lawrence Livermore National Laboratory`s (LLNL`s) diverse expertise to develop a geophysical monitoring system that can survive being dropped into place by a helicopter or airplane. Such an air-deployable system could significantly decrease the time and effort needed to set up such instruments in remote locations following a major earthquake or volcanic eruption. Most currently available geophysical monitoring and survey systems, such as seismic monitoring stations, use sensitive, fragile instrumentation that requires personnel trained and experienced in data acquisition and field setup. Rapid deployment of such equipment can be difficult or impossible. Recent developments in low-power electronics, new materials, and sensors that are resistant to severe impacts have made it possible to develop low-cost geophysical monitoring packages for rapid deployment missions. Our strategy was to focus on low-cost battery-powered systems that would have a relatively long (several months) operational lifetime. We concentrated on the conceptual design and engineering of a single-component seismic system that could survive an air-deployment into an earth material, such as alluvium. Actual implementation of such a system is a goal of future work on this concept. For this project, we drew on LLNL`s Earth Sciences Department, Radio Shop, Plastics Shop, and Weapons Program. The military has had several programs to develop air-deployed and cannon-deployed seismometers. Recently, a sonobuoy manufacturer has offered an air-deployable geophone designed to make relatively soft landings.
Abstract 4295: INT230-6, a novel intratumoral anticancer agent, is able to eradicate large established tumors and stimulate potent anti tumor immunity (2015)

Abstract Historically, intratumoral (IT) administered chemotherapy has not been shown to provide better efficacy than the same drugs delivered intravenously. This is postulated to be due to poor drug diffusion and dispersion throughout the tumor. Intensity has developed a novel formulation of cytotoxic chemotherapy plus a penetration enhancer which improves drug diffusion and uptake into the cancer cell. INT230-6 is a combination of low dose cisplatin, vinblastine and a cell penetration excipient. This technology enables more rapid and improved penetration throughout the tumor including hypoxic and un-vascularized areas. Cohorts of 10 BALB/c mice were inoculated with 1×106 CT26 cells subcutaneously (SC). Tumors were grown to a mean of 325mm3 and then treatment with INT230-6 was administered IT daily for a single cycle of 5 days. Control arms using comparable size tumors pre-dose included no treatment, IV cisplatin and vinblastine, and IT injection of these drugs without the enhancer. INT230-6 treatment resulted in regression from baseline in all animals with a complete regression of the large tumors in 20%. Many tumors continued to shrink in size well beyond multiple half-lives of the chemotherapy and excipient implying an anti-tumor effect not due to direct cytotoxicity from the drugs. INT230-6 administration resulted in a significant extension of overall survival compared to all controls. Notably complete responders with this treatment were resistant to 3 separate attempts at re-inoculation with 1×106 CT26 cells by SC or IV administration without retreatment of drug, while control animals rapidly developed metastatic tumors and died. This apparent tumor immunity persisted for &amp;gt; 440 days suggesting permanent immunity. In a follow-on study, tumors of some animals receiving INT230-6 were shown to be 75% necrotic on day 3 of treatment with an observed increase in the density of CD4 positive cells and CD11c positive cells in the treated tumors on day 10 versus controls. These changes might be involved in the induction of immunity. These results suggest that intratumoral administration of INT230-6 creates the proper tumor cell apoptosis kinetics and antigen presentation to enable a personalized adaptive immune response to develop. Further work is being done to look at repeat dosing and combinations with other immunomodulating agents. Citation Format: Ian B. Walters, Lewis H. Bender, Masaki Terabe, Jay A. Berzofsky. INT230-6, a novel intratumoral anticancer agent, is able to eradicate large established tumors and stimulate potent anti tumor immunity. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 4295. doi:10.1158/1538-7445.AM2015-4295
Isolation and Characterization of Mini-Tn 5 Km2 Insertion Mutants of Enterohemorrhagic Escherichia coli O157:H7 Deficient in Adherence to Caco-2 Cells (2000)

Adherence of enterohemorrhagic Escherichia coli (EHEC) to intestinal epithelium is essential for initiation of the infection. To identify genes involved in adherence, an EHEC O157:H7 strain (O157Sakai) was mutagenized by mini-Tn5Km2, where Km refers to kanamycin resistance, and 4,677 insertion mutants were screened for their ability to form microcolonies (MC) on Caco-2 cells. The less adherent mutants were divided into three groups: those with no adherent ability (designated as class 1 mutants, n = 10), those less adherent than the wild type (class 2 mutants, n = 16), and those unable to form MC but which adhered in a diffuse manner (class 3 mutants, n = 1). The sites of insertion in class 1 mutants were all found within genes of the locus for enterocyte effacement (LEE) thought to be required for type III protein secretion. Indeed, the class 1 mutants failed to secrete type III secreted proteins such as EspA and Tir into the culture medium. The insertions in class 2 mutants were outside the LEE, and all the mutants except one were able to secrete type III proteins into the culture medium. The class 3 mutant had the insertion in the tir gene in the LEE and was deficient in Tir and intimin expression, suggesting that in the absence of intimin-Tir, O157Sakai can still adhere to Caco-2 cells but in a diffused manner. This was confirmed by construction of a nonpolar eae (encoding intimin) mutant. Examination of the eae mutant together with O157Sakai and one of the class 1 mutants for the ability to form MC revealed that EHEC initially adhered diffusely at 1.5 h after infection. Following washing out of the nonadherent bacteria, while wild-type EHEC bacteria developed MC for another 2 to 3 h on Caco-2 cells, the eae mutant diffusely adhered throughout the infection without forming MC. MC with O157Sakai but not the diffusely adherent eae mutant could evoke F-actin condensation beneath the bacterium. Our results suggest that EHEC encodes additional adherence-associated loci and that the type III secreted proteins are involved in the initial diffuse adherence, while the intimin-Tir interaction is required for the subsequent development of MC.
New Radio (NR) and its Evolution toward 5G-Advanced (2019)

New Radio (NR), which is part of Release 15 of Third Generation Partnership Project (3GPP) standards, marks the first fifth generation (5G) standards designed to meet the requirements set forth by the International Telecommunication Union for IMT-2020. This article presents an overview of the NR radio interface and the ongoing work in 3GPP to evolve NR toward 5G-Advanced, allowing it to access new spectrum and support a broader range of vertical services while enhancing its performance beyond what is available today.
Improving Saudi Class G Oil-Well Cement Properties Using the Tire Waste Material (2020)

After oil and gas well drilling, they should be cased and cemented to ensure the stability of the wellbore and to isolate the trouble zones. To achieve these jobs, several additives are incorporated into the cement slurry to improve the cement matrix durability, especially at temperatures above 230 F. The tire waste material is an industrial waste that comes from automobile tires. The purpose of this work is to investigate the prospect of utilizing tire waste in oil-well cement under high-temperature and high-pressure conditions of 292 F and 3000 psi. Three cement samples with different concentrations of the tire waste material were prepared. The effects of tire waste on the cement rheological properties, elastic and failure parameters, and permeability were examined. The results showed that adding 0.3% by weight of cement (BWOC) of the tire waste material considerably improved the cement to the cement slurry and cement matrix properties, and it decreased the cement plastic viscosity by 53.1% and increased its yield point by 142.4% compared to the base cement. The cement samples with 0.3% BWOC of tire waste have Young's modulus which is 10.8% less than that of the base cement and Poisson's ratio of 14.3% greater than that of the base cement. By incorporating 0.3% of the tire waste, both compressive and tensile strengths of the cement increased by 48.3 and 11.7%, respectively, compared with those of the base cement. The cement permeability was decreased by 66.0% after adding 0.3% of the tire waste. Besides the improvement in the properties of cement, the use of the tire waste material has other economical and environmental advantages because these are very cheap materials dominant in our life.
Cancer dormancy and criticality from a game theory perspective (2018)

We determine to what extent the time-dependence of the populations can be quantitively understood in spite of the underlying complexity of the individual agents and model the phenomena of dormancy.
Measurement of multi-particle azimuthal correlations in pp, p + Pb and low-multiplicity Pb + Pb collisions with the ATLAS detector (2017)

Multi-particle cumulants and corresponding Fourier harmonics are measured for azimuthal angle distributions of charged particles in [Formula: see text] collisions at [Formula: see text] = 5.02 and 13 TeV and in [Formula: see text] + Pb collisions at [Formula: see text] = 5.02 TeV, and compared to the results obtained for low-multiplicity [Formula: see text] collisions at [Formula: see text] = 2.76 TeV. These measurements aim to assess the collective nature of particle production. The measurements of multi-particle cumulants confirm the evidence for collective phenomena in [Formula: see text] + Pb and low-multiplicity [Formula: see text] collisions. On the other hand, the [Formula: see text] results for four-particle cumulants do not demonstrate collective behaviour, indicating that they may be biased by contributions from non-flow correlations. A comparison of multi-particle cumulants and derived Fourier harmonics across different collision systems is presented as a function of the charged-particle multiplicity. For a given multiplicity, the measured Fourier harmonics are largest in [Formula: see text], smaller in [Formula: see text] + Pb and smallest in [Formula: see text] collisions. The [Formula: see text] results show no dependence on the collision energy, nor on the multiplicity.
[Experiences with computer-assisted x-ray diagnosis of pulmonary coin lesions (author's transl)]. (1976)

Favourable results of the computer-aided diagnosis of peripheral bronchial carcinoma lead to a simplified scheme for calculation fo diagnosis, basing upon X-ray symptoms. This scheme was tested in the Zentralinstitut für Krebsforschung (ZIK) in 102 pateints and in the Forschungsinstitut für Lungenkrankheiten und Tuberkulose (FLT) in 101 patients. These patients had coin lesions of the lung with unknown diagnoses. The histological diagnoses later proved were compared with the calculated diagnoses. In ZIK there were correct diagnosis in 92.16% (in 96.53% of the carcinomas, in 44.4% of the tuberculomas and in all benign tumors), while in FLT 85.53% of the coin lesions were correctly classified (89.13% of carcinomas, 79.31% of the tuberculomas and all benign tumors). The comparison with the clinical-histological diagnoses showed a clear improvement of the accuracy by the calculated diagnoses. The method can be recommended for the basic diagnosis especially of peripheral bronchial carcinoma. It implies a reduction of the diagnostic delay, but it can not substitute the histologic investigation of the lesions.
PNEUMATIC HAMMER SOIL VAPOR PROBES AND MINIATURE PIEZOMETERS FOR GASOLINE SPILL DELINEATION AND CLEANUP (1989)

ABSTRACT In a new twist on an old procedure, pneumatically injected miniature well points may change soil investigations much as nail guns have changed carpentry. Soil vapor analysis has increasingly gained attention as a means of locating and monitoring subsurface gasoline spills. Accurate depiction of the plume position requires reproducible vapor detection and monitoring. In addition, static water level needs to be determined to understand flow direction. Miniature aluminum slitted well points can be mass-produced by computer-aided production facilities to create low-cost identical copies about 10 cm in length. The small points can be inserted into shafts and injected into the ground. The shaft is then pulled back, leaving a flexible teflon or polypropylene tubing attached. A gasoline spill at Falmouth, Cape Cod, Massachusetts, provided an opportunity to test the use of the injected “shield” points. More than 40 vapor points were installed in two days during four transects across a product plume located 10 m below grade.