gebhart/patrepeval huggingface dataset:
Cloning and expression of two pab genes coding for p-aminobenzoic acid synthase, an enzyme involved in candicidin biosynthesis (1984)

Conference Article| October 01 1984 Cloning and expression of two pab genes coding for p-aminobenzoic acid synthase, an enzyme involved in candicidin biosynthesis JOSÉ A. GIL; JOSÉ A. GIL 1Departamento de Microbiología, Facultad de Biología, Universidad de León, Spain Search for other works by this author on: This Site PubMed Google Scholar ANGELITA REBOLLO; ANGELITA REBOLLO 1Departamento de Microbiología, Facultad de Biología, Universidad de León, Spain Search for other works by this author on: This Site PubMed Google Scholar TERE ALEGRE; TERE ALEGRE 1Departamento de Microbiología, Facultad de Biología, Universidad de León, Spain Search for other works by this author on: This Site PubMed Google Scholar PALOMA LIRAS; PALOMA LIRAS 1Departamento de Microbiología, Facultad de Biología, Universidad de León, Spain Search for other works by this author on: This Site PubMed Google Scholar JUAN F. MARTÍN JUAN F. MARTÍN 1Departamento de Microbiología, Facultad de Biología, Universidad de León, Spain Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1984) 12 (5): 865. https://doi.org/10.1042/bst0120865 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation JOSÉ A. GIL, ANGELITA REBOLLO, TERE ALEGRE, PALOMA LIRAS, JUAN F. MARTÍN; Cloning and expression of two pab genes coding for p-aminobenzoic acid synthase, an enzyme involved in candicidin biosynthesis. Biochem Soc Trans 1 October 1984; 12 (5): 865. doi: https://doi.org/10.1042/bst0120865 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: kb, kilobase; PABA, p-aminobenzoic acid This content is only available as a PDF. © 1984 Biochemical Society1984 Article PDF first page preview Close Modal You do not currently have access to this content.
Analysis of a shielded writer for perpendicular recording (2004)

In this paper, we analyzed the properties of shielded writers, including both theoretical and experiment analysis. It is shown for example, that thinner pole tips can be used, thus reducing non-zero skew-angle sensitivity as compared to the regular SPH structure.
Alternative Reagents for Methotrexate as Immobilizing Anchor Moieties in the Optimization of MASPIT: Synthesis and Biological Evaluation (2015)

We report the evaluation of two alternative chemical dimerizer approaches aimed at increasing the sensitivity of MASPIT, a three-hybrid system that enables small-molecule target protein profiling in intact human cells. To circumvent the potential limitations related to the binding of methotrexate (MTX) to endogenous human dihydrofolate reductase (DHFR), we explored trimethoprim (TMP) as an alternative prokaryote-specific DHFR ligand. MASPIT evaluation of TMP fusion compounds with tamoxifen, reversine, and simvastatin as model baits, resulted in dose-response curves shifted towards lower EC50 values than those of their MTX congeners. Furthermore, a scalable azido-TMP reagent was synthesized that displayed a similar improvement in sensitivity, possibly owing to increased membrane permeability relative to the MTX anchor. Applying the SNAP-tag approach to introduce a covalent bond into the system, on the other hand, produced an inferior readout than in the MTX- or TMP-tag based assay.
P‐48: Development of Novel RGBW Mobile Display with Local Dimming Backlight System (2014)

Abstract We have developed a new 7‐inch WQXGA RGBW mobile LCD with local dimming backlight technology. This panel achieves a 20% power reduction compared to that of the prior RGBW displays, with no image quality degradation. It also realizes contrast improvement and a total module thickness of 1.16mm.
A neuro-fuzzy approach to estimation and control in automotive prepaint process (2002)

We discuss the process of design and implementation of a neuro-fuzzy control system. This system combines an estimation and a control algorithm that deals with the problem of control of one important process variable in the automotive prepaint process-the nitrite. A neuro-fuzzy nitrite control system that has been implemented in an automotive assembly plant is presented.
Integrated Genomic Analysis Identifies Clinically Relevant Subtypes of Glioblastoma Characterized by Abnormalities in PDGFRA, IDH1, EGFR, and NF1 (2019)

The Cancer Genome Atlas Network recently cataloged recurrent genomic abnormalities in glioblastoma multiforme (GBM). We describe a robust gene expression-based molecular classification of GBM into Proneural, Neural, Classical, and Mesenchymal subtypes and integrate multidimensional genomic data to establish patterns of somatic mutations and DNA copy number. Aberrations and gene expression of EGFR, NF1, and PDGFRA/IDH1 each define the Classical, Mesenchymal, and Proneural subtypes, respectively. Gene signatures of normal brain cell types show a strong relationship between subtypes and different neural lineages. Additionally, response to aggressive therapy differs by subtype, with the greatest benefit in the Classical subtype and no benefit in the Proneural subtype. We provide a framework that unifies transcriptomic and genomic dimensions for GBM molecular stratification with important implications for future studies.
Optically switchable transistors by simple incorporation of photochromic systems into small-molecule semiconducting matrices (2015)

The fabrication of multifunctional high-performance organic thin-film transistors as key elements in future logic circuits is a major research challenge. Here we demonstrate that a photoresponsive bi-functional field-effect transistor with carrier mobilities exceeding 0.2 cm(2) V(-1) s(-1) can be developed by incorporating photochromic molecules into an organic semiconductor matrix via a single-step solution processing deposition of a two components blend. Tuning the interactions between the photochromic diarylethene system and the organic semiconductor is achieved via ad-hoc side functionalization of the diarylethene. Thereby, a large-scale phase-segregation can be avoided and superior miscibility is provided, while retaining optimal π-π stacking to warrant efficient charge transport and to attenuate the effect of photoinduced switching on the extent of current modulation. This leads to enhanced electrical performance of transistors incorporating small conjugated molecules as compared with polymeric semiconductors. These findings are of interest for the development of high-performing optically gated electronic devices.
A multiresolution image reconstruction method in X-ray microCT (2009)

We propose a method for multiresolution image reconstruction in X-ray micro computed tomography (microCT). It can have a variety of applications, from material characterization to small animal imaging studies. The main idea is to recover an overall image of the sample with a coarse resolution, and with a fine resolution for a region-of-interest (ROI). In a zoo-min CT type setup, two sets of data are used, taken at different magnifications ratios. They are combined with the help of an analytical relation and the reconstruction is an extension of the filtered back-projection (FBP) algorithm. We present results with simulated data, some performance aspects and a simple noise analysis.
Improving infrastructure delivery though better use of standards (2015)

Inefficient and inconsistent use of codes and standards can hamper effective delivery of infrastructure projects. Steven Wilson of BSI, Bill Grose of High Speed Two (HS2) and Colin Rawlings of CH2MHill/HS2 outline initiatives on the HS2 project to deliver successful standards.
Characterization of the diversity of rhizosphere fluorescent pseudomonads populations with lipoproteine (OPRI) gene and siderphore polymorphism (1992)

International audience
Structural Characterization of Biphenyl Ester-Based LC Molecules:  Peculiarities of Cyclic Siloxane-Based Materials (1996)

The X-ray meridional profiles of a series of liquid crystalline molecules possessing the same mesogen but different siloxane-based backbones and spacer lengths are compared. Siloxane cores investigated include rings of different sizes, linear polysiloxanes, and short linear segments. The presence of diffuse, periodic reflections for ring-based compounds is attributed in part to translational association of the mesogenic units along the director (strings). The absence of these reflections in smaller, more flexible cores indicates that the localization of strong scattering units induced by the cyclic and linear cores also contributes to the strength of this molecular association. Correlations between measured correlation lengths, orientation parameters, and “string” lengths are discussed in detail. Preliminary molecular modeling is used to investigate the origins of these peculiar scattering phenomena as a function of the molecular architecture.
PWM inverters and their influence on motor overvoltage (2002)

Modern voltage source pulse width modulation (PWM) inverters have elevated adjustable speed AC drives to a performance level comparable to DC drives. To accomplish this, voltage source inverters (VSI) now employ third generation insulated gate bipolar transistors (IGBT); however, the fast rise times associated with these devices produce unintended consequences. This paper examines motor overvoltages resulting from IGBT PWM VSI. Previous investigations have shown a critical power cable distance exists beyond which twice bus voltage (2 pu) terminal voltages are possible. This is determined primarily by the rise time of the devices and cable characteristics. This paper investigates motor voltages >2 pu, their cause and solution. In contrast to motor voltages /spl ges/2 pu, the paper shows motor voltages >2 pu primarily are determined by the PWM carrier frequency and modulation strategy. Furthermore, the paper demonstrates motor voltages up to 3-4 pu are possible. Software modifications to the PWM modulator and modulating signal are presented to reduce >2 pu motor overvoltage. Additionally, a hardware correction, easily implemented within a field programmable gate array (FPGA), is disclosed and experimentally demonstrated.
Memory CD4 + T-Lymphocyte Loss and Dysfunction during Primary Simian Immunodeficiency Virus Infection (2007)

It has long been appreciated that CD4+ T lymphocytes are dysfunctional in human immunodeficiency virus (HIV)/simian immunodeficiency virus (SIV)-infected individuals, and it has recently been shown that HIV/SIV infections are associated with a dramatic early destruction of memory CD4+ T lymphocytes. However, the relative contributions of CD4+ T-lymphocyte dysfunction and loss to immune dysregulation during primary HIV/SIV infection have not been fully elucidated. In the current study, we evaluated CD4+ T lymphocytes and their functional repertoire during primary SIVmac251 infection in rhesus monkeys. We show that the extent of loss of memory CD4+ T lymphocytes and staphylococcal enterotoxin B-stimulated cytokine production by total CD4+ T lymphocytes during primary SIVmac251 infection is tightly linked in a cohort of six rhesus monkeys to set point plasma viral RNA levels, with greater loss and dysfunction being associated with higher steady-state viral replication. Moreover, in exploring the mechanism underlying this phenomenon, we demonstrate that the loss of functional CD4+ T lymphocytes during primary SIVmac251 infection is associated with both a selective depletion of memory CD4+ T cells and a loss of the functional capacity of the memory CD4+ T lymphocytes that escape viral destruction.
Rights Management Technologies: A Good Choice for Securing Electronic Health Records? (2007)

Advances in healthcare IT bring new concerns with respect to privacy and security. Security critical patient data no longer resides on mainframes physically isolated within an organization, where physical security measures can be taken to defend the data and the system. Modern solutions are heading towards open, interconnected environments where storage outsourcing and operations on untrusted servers happen frequently. In order to allow secure sharing of health records between different healthcare providers, Rights Management Techniques facilitating a datacentric protection model can be employed: data is cryptographically protected and allowed to be outsourced or even freely float on the network. Rather than relying on different networks to provide confidentiality, integrity and authenticity, data is protected at the end points of the communication. In this paper we compare Enterprise/Digital Rights Management with traditional security techniques and discuss how Rights Management can be applied to secure Electronic Health Records.
ML418: The First Selective, Sub-Micromolar Pore Blocker of Kir7.1 Potassium Channels (2016)

The inward rectifier potassium (Kir) channel Kir7.1 (KCNJ13) has recently emerged as a key regulator of melanocortin signaling in the brain, electrolyte homeostasis in the eye, and uterine muscle contractility during pregnancy. The pharmacological tools available for exploring the physiology and therapeutic potential of Kir7.1 have been limited to relatively weak and nonselective small-molecule inhibitors. Here, we report the discovery in a fluorescence-based high-throughput screen of a novel Kir7.1 channel inhibitor, VU714. Site-directed mutagenesis of pore-lining amino acid residues identified glutamate 149 and alanine 150 as essential determinants of VU714 activity. Lead optimization with medicinal chemistry generated ML418, which exhibits sub-micromolar activity (IC50 = 310 nM) and superior selectivity over other Kir channels (at least 17-fold selective over Kir1.1, Kir2.1, Kir2.2, Kir2.3, Kir3.1/3.2, and Kir4.1) except for Kir6.2/SUR1 (equally potent). Evaluation in the EuroFins Lead Profiling panel of 64 GPCRs, ion-channels, and transporters for off-target activity of ML418 revealed a relatively clean ancillary pharmacology. While ML418 exhibited low CLHEP in human microsomes which could be modulated with lipophilicity adjustments, it showed high CLHEP in rat microsomes regardless of lipophilicity. A subsequent in vivo PK study of ML418 by intraperitoneal (IP) administration (30 mg/kg dosage) revealed a suitable PK profile (Cmax = 0.20 μM and Tmax = 3 h) and favorable CNS distribution (mouse brain/plasma Kp of 10.9 to support in vivo studies. ML418, which represents the current state-of-the-art in Kir7.1 inhibitors, should be useful for exploring the physiology of Kir7.1 in vitro and in vivo.
Author response: Mapping the functional versatility and fragility of Ras GTPase signaling circuits through in vitro network reconstitution (2016)

Cells sense and respond to the world around them using signaling “circuits” made of proteins and other molecules, and when an important cell circuit breaks, diseases like cancer may arise. Much like with electrical circuits, a given set of molecular components can be used to build different signaling circuits that behave in different ways. However, unlike for electrical circuits we generally do not have design manuals that allow us to work out how a signaling circuit behaves based on the components it includes. Doing this would involve identifying all the molecular parts of a circuit, using them to build every possible circuit, and carefully measuring the associated behavior. A group of proteins called the Ras-superfamily GTPases are important controllers of cell behavior. To investigate the behavior of Ras GTPase signaling circuits, Coyle and Lim built up different circuits from their components and “watched” their behavior with a microscope. Analyzing these behaviors provided the information needed to produce a ‘design manual’ for programming Ras circuits. Coyle and Lim found that the makeup of a Ras signaling circuit strongly affects the timing, duration, shape and size of its output. This means that different cells can use the same core components in different ways to build circuits customized to their specific needs. Nonetheless, this versatility comes with a trade-off: the circuits are fragile, and can break in many different ways to cause disease. In the future Coyle and Lim aim to build other types of important cellular signaling circuits from their component parts. Only by building these systems, turning them on and watching them run can we begin to understand how they actually perform and what they are capable of.
ChemInform Abstract: Novel and Efficient Synthesis of 4‐Sulfonyl‐2‐pyridones. (2009)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Association between the CHRM2 gene and intelligence in a sample of 304 Dutch families (2006)

The CHRM2 gene is thought to be involved in neuronal excitability, synaptic plasticity and feedback regulation of acetylcholine release and has previously been implicated in higher cognitive processing. In a sample of 667 individuals from 304 families, we genotyped three single-nucleotide polymorphisms (SNPs) in the CHRM2 gene on 7q31-35. From all individuals, standardized intelligence measures were available. Using a test of within-family association, which controls for the possible effects of population stratification, a highly significant association was found between the CHRM2 gene and intelligence. The strongest association was between rs324650 and performance IQ (PIQ), where the T allele was associated with an increase of 4.6 PIQ points. In parallel with a large family-based association, we observed an attenuated - although still significant - population-based association, illustrating that population stratification may decrease our chances of detecting allele-trait associations. Such a mechanism has been predicted earlier, and this article is one of the first to empirically show that family-based association methods are not only needed to guard against false positives, but are also invaluable in guarding against false negatives.
Approaches to β-Fused Porphyrinoporphyrins: Pyrrolo- and Dipyrromethaneporphyrins (1997)

Reaction of (2-nitro-5,10,15,20-tetraphenylporphyrinato)nickel(II) 3 or its copper(II) complex 4 with α-isocyanoacetic esters in the presence of 1,8-diazabicyclo[5.4.O]undec-7-ene accomplishes the first syntheses of β-fused pyrroloporphyrins (e.g. 5–10). The removal of the fused pyrrole ester protecting groups to give the di-α-free pyrroloporphyrins 2a and 13 is described, as well as some pyrrole-type chemistry. Unsymmetrical fused dipyrromethanoporphyrins (16–18) were synthesized by reacting 5-acetoxymethylpyrrole-2-carboxylates (14,15) with mono-α-free pyrroloporphyrins (5–8) in the presence of Montmorillonite clay. A symmetrical bis-porphyrinyl dipyrromethane 19 was also prepared by condensation of the β-fused methyl ester pyrroloporphyrin 7 with dimethoxymethane in ethanol and concentrated hydrochloric acid.
A Super-Oxidized Radical Cationic Icosahedral Boron Cluster (2020)

While the icosahedral closo-[B12H12]2– cluster does not display reversible electrochemical behavior, perfunctionalization of this species via substitution of all twelve B–H vertices with alkoxy orbenzyloxy (OR) substituents engenders reversible redox chemistry, providing access to clusters in the dianionic,monoanionic, and neutral forms. Here, we evaluated the electrochemical behavior of the electron-rich B12(O-3-methylbutyl)12 (1) cluster and discovered that a new reversible redox event that gives rise to a fourth electronic state is accessible through one-electron oxidation of the neutral species. Chemical oxidation of 1 with [N(2,4-Br2C6H3)3]•+ afforded the isolable[1] •+ cluster, which is the first example of an open-shell cationic B12 cluster in which the unpaired electron is proposed to be delocalized throughout the boron cluster core. The oxidation of 1 is also chemically reversible, where treatment of [1]•+ with ferrocene resulted in its reduction back to 1. The identity of [1]•+ is supported by EPR, UV-vis, multinuclear NMR (1H, 11B), and X-ray photoelectron spectroscopic characterization.&lt;br&gt;
Group V Phospholipase A2 in Bone Marrow-derived Myeloid Cells and Bronchial Epithelial Cells Promotes Bacterial Clearance after Escherichia coli Pneumonia (2011)

Group V-secreted phospholipase A(2) (GV sPLA(2)) hydrolyzes bacterial phospholipids and initiates eicosanoid biosynthesis. Here, we elucidate the role of GV sPLA(2) in the pathophysiology of Escherichia coli pneumonia. Inflammatory cells and bronchial epithelial cells both express GV sPLA(2) after pulmonary E. coli infection. GV(-/-) mice accumulate fewer polymorphonuclear leukocytes in alveoli, have higher levels of E. coli in bronchoalveolar lavage fluid and lung, and develop respiratory acidosis, more severe hypothermia, and higher IL-6, IL-10, and TNF-α levels than GV(+/+) mice after pulmonary E. coli infection. Eicosanoid levels in bronchoalveolar lavage are similar in GV(+/+) and GV(-/-) mice after lung E. coli infection. In contrast, GV(+/+) mice have higher levels of prostaglandin D(2) (PGD(2)), PGF(2α), and 15-keto-PGE(2) in lung and express higher levels of ICAM-1 and PECAM-1 on pulmonary endothelial cells than GV(-/-) mice after lung infection with E. coli. Selective deletion of GV sPLA(2) in non-myeloid cells impairs leukocyte accumulation after pulmonary E. coli infection, and lack of GV sPLA(2) in either bone marrow-derived myeloid cells or non-myeloid cells attenuates E. coli clearance from the alveolar space and the lung parenchyma. These observations show that GV sPLA(2) in bone marrow-derived myeloid cells as well as non-myeloid cells, which are likely bronchial epithelial cells, participate in the regulation of the innate immune response to pulmonary infection with E. coli.
Electrical components library for HAWC2 (2007)

The work presented in this report is part of the EFP project called "A Simulation Platform to Model, Optimize and Design Wind Turbines" partly funded by the Danish Energy Authority under contract number 1363/04-0008. The project is carried out in cooperation between Risø National Laboratory and Aalborg University. In this project, the focus is on the development of a simulation platform for wind turbine systems using different simulation tools. This report presents the electric component library developed for use in the aeroelastic code HAWC2. The developed library includes both steady state and dynamical models for fixed and variable speed wind turbines. A simple steady-state slip model was developed for the fixed speed wind turbine. This model is suitable for aeroelastic design of wind turbines under normal operation. A dynamic model of an induction generator for the fixed speed wind turbine was developed. The model includes the dynamics of the rotor fluxes. The model is suitable for a more detailed investigation of the mechanical - electrical interaction, both under normal and fault operation. For the variable speed wind turbine, a steadystate model, typically used in aeroelastic design, was implemented. The model can be used for normal and, to some extent, for fault operation. The reduced order dynamic model of a DFIG was implemented. The model includes only the active power controller and can be used for normal operation conditions.