gebhart/patrepeval huggingface dataset:
Abstract B14: Anti-miR-135b in colon cancer treatment (2012)

Abstract Background: MicroRNAs (miRs) are small non coding RNAs involved in cell homeostasis. miRs are deregulated in colorectal cancer (CRC). Our study aimed at identifying miRs with a driver role in carcinogenesis altered by similar mechanisms in both human and mouse CRC. Goal of the study was to use CRC mouse models for the pre-clinical development of anti-miRs as therapeutic drugs. Methods: Azoximetane (AOM)/Dextran-Sulfate (DSS) treated mice or CDX2Cre-APC f/wt mice were used to study inflammation-associated and sporadic APC-related CRC. Human Inflammatory Bowel Disease associated (n=30), and sporadic (n=90) CRC with their matched normal tissues were collected according to Good Clinical Practice recommendation and subjected to RNA extraction using Trizol. miR and gene expression profiling was assessed by nCounter technology (Nanostring Seattle). AntimiR-135b and scrambled probes for in vivo studies were synthesized by Girindus. Results: miRs profiling from AOM/DSS and CDX2Cre-APC f/wt CRC. revealed that miR-135b is one of the most up-regulated miRs in both models. In humans miR-135b over-expression was found in both IBD and sporadic CRC and was associated with reduced Progression Free Survival and Overall Survival in CRC patients. Molecular studies in Mouse Embryo Fibroblast and human CRC cell lines highlighted the role of two major pathways in the upstream activation of miR-135b: APC-β-Catenin and SRC-PI3K. MiR-135b up-regulation resulted in reduced apoptosis and increased invasion and metastasis due to the down-regulation of TGFRB2, DAPK1, APC and HIF1AN. Silencing of miR-135b in vivo reduced tumor multiplicity and tumor load in the AOM/DSS CRC model. Mice treated with anti-miR-135b showed well differentiated tumors and acinar pattern while tumors in the control groups showed low differentiation and adenomatous pattern. Conclusions: Our data suggest that miR-135b is a key molecule whose activation is downstream of oncogenes and oncosuppressor genes frequently altered in CRC. Our study defines specific pathways that converge on the activation of the same microrna. The “in vivo” silencing of miR-135 shows preclinical efficacy with low toxicity and represents the first in vivo study for the use of antimiRs in CRC treatment Note: This abstract was not presented at the conference because the presenter was unable to attend. Citation Format: Nicola Valeri, Roberta Gafa', Gerard Nuovo, Giovanni Lanza, Wendy Frankel, Peter K. Vogt, Joanna Groden, Michael Karin, Carlo M. Croce, Chiara Braconi, Pierluigi Gasparini, Sergei Grivennikov, Jonathan R. Hart, Alessio Paone, Francesca Lovat, Muller Fabbri. Anti-miR-135b in colon cancer treatment [abstract]. In: Proceedings of the AACR Special Conference on Noncoding RNAs and Cancer; 2012 Jan 8-11; Miami Beach, FL. Philadelphia (PA): AACR; Cancer Res 2012;72(2 Suppl):Abstract nr B14.
[2-3H]Mannose-labeling and Analysis of N-linked Oligosaccharides (2017)

Modifications of N-linked oligosaccharides of glycoproteins soon after their biosynthesis correlate to glycoprotein folding status. These alterations can be detected in a sensitive way by pulse-chase analysis of [2-<sup>3</sup>H]mannose-labeled glycoproteins, with enzymatic removal of labeled N-glycans, separation according to size by HPLC and radioactive detection in a scintillation counter.
Dependence of polarization-mode dispersion penalties on receiver characteristics (2002)

We measured the dependence of polarization-mode dispersion (PMD) penalty on receiver characteristics in an optically preamplified receiver using nonreturn-to-zero (NRZ) and return-to-zero (RZ) optical signals. The PMD-induced penalties were measured as a function of receiver electrical bandwidth at two different conditions: an optimized decision point and a fixed decision point. We also describe the measurement of the input polarization dependence of the PMD-induced penalty using polarization-scrambled signals. The observed penalty depends strongly on decision point and receiver electrical bandwidth in both NRZ and RZ signal formats.
Motion-adapted Three-dimensional Frequency Selective Extrapolation (2019)

It has been shown, that high resolution images can be acquired using a low resolution sensor with non-regular sampling. Therefore, post-processing is necessary. In terms of video data, not only the spatial neighborhood can be used to assist the reconstruction, but also the temporal neighbor-hood. A popular and well performing algorithm for this kind of problem is the three-dimensional frequency selective extrapolation (3D-FSE) for which a motion adapted version is introduced in this paper. This proposed extension solves the problem of changing content within the area considered by the 3D-FSE, which is caused by motion within the sequence. Because of this motion, it may happen that regions are emphasized during the reconstruction that are not present in the original signal within the considered area. By that, false content is introduced into the extrapolated sequence, which affects the resulting image quality negatively. The novel extension, presented in the following, incorporates motion data of the sequence in order to adapt the algorithm accordingly, and compensates changing content, resulting in gains of up to 1.75 dB compared to the existing 3D-FSE.
Exploring the Effect of Iron Metal-Organic Framework Particles in Polylactic Acid Membranes for the Azeotropic Separation of Organic/Organic Mixtures by Pervaporation (2021)

A microporous carboxylate metal-organic framework MIL-100 Fe was prepared as submicron particles by microwave-assisted hydrothermal synthesis (Fe-MOF-MW). This product was explored, for the first time, for the preparation of polylactic acid (PLA) mixed matrix membranes. The produced MOF was characterised by powder X-ray diffraction (PXRD), environmental scanning electron microscopy (ESEM) as well as by thermogravimetric analysis (TGA) and nitrogen adsorption/desorption. The effect of different Fe-MOF-MW concentrations (0.1 and 0.5 wt%) on the membrane properties and performance were evaluated. These membranes were used in the pervaporation process for the separation of methanol/methyl <i>tert</i>-butyl-ether mixtures at the azeotropic point. The influence of the feed temperature and vacuum pressure on the membrane performance was evaluated and the results were compared with PLA pristine membranes. Moreover, the produced membranes have been characterised in terms of morphology, MOF dispersion in the polymeric membrane matrix, wettability, thickness, mechanical resistance and swelling propensity. The presence of Fe-MOF-MW was found to have a beneficial effect in improving the selectivity of mixed matrix membranes towards methanol at both concentrations. The highest selectivity was obtained for the PLA membranes embedded with 0.5 wt% of Fe-MOF-MW and tested at the temperature of 25 °C and vacuum pressure of 0.09 mbar.
Assembly of lipid‐containing viruses (1974)

Abstract Enveloped viruses which form by budding at the cell surface possess a membrane consisting of a lipid bilayer and a small number of virus‐coded polypeptides. Since viral polypeptides become integral components of the plasma membrane during assembly, the process of synthesis and incorporation into membranes of these proteins may reflect the pathway of plasma membrane assembly. Electron microscopic studies have suggested that viral envelope proteins are incorporated into discrete, localized regions of the plasma membrane which serve as recognition sites for the viral nucleocapsid. In influenza virus‐infected cells, viral polypeptides are associated with cytoplasmic membranes as well as the plasma membrane. The major envelope glycoprotein appears to be synthesized in rough endoplasmic reticulum, and to migrate to smooth membranes after synthesis. Glycosylation is initiated in rough membranes and progresses further in smooth membranes. Unlike the glycoproteins, the major nonglycosylated polypeptide appears to be inserted directly into the plasma membrane. In the presence of 2‐deoxyglucose or high concentrations of glucosamine, aberrant viral glycoproteins are detected which appear to be unglycosylated or partially glycosylated; these are associated with membranes and incorporated into virus particles of reduced infectivity. Therefore normal glycosylation is not essential for incorporation of viral glycoproteins into cellular membranes or virus particles, but is required for normal biological activity. The role of the viral neuraminidase in assembly and release has been studied using mutants defective in neuraminidase at restrictive temperature. Under these conditions virus formation occurs, but the progeny form large aggregates at the cell surface. Colloidal iron hydroxide staining indicates that such virus particles contain neuraminic acid, and these residues appear to serve as receptors leading to the extensive aggregation.
Determination and Origin of the Aroma Impact Compounds of Yogurt Flavor (1997)

Yogurt exhibits a delicate and low intense flavor which requires mild sample isolation techniques and sensitive identification means. This paper decribes the application of two new flavor separation techniques: combined static and dynamic headspace, and preparative simultaneous distillation−extraction under vacuum. Of the 91 components identified by mass spectrometry, 21 were found to have a major impact on the aroma using a new method for the treatment of GC−sniffing data. One of these compounds, 1-nonen-3-one, was identified by spectral means for the first time in a flavor. Its odor detection threshold of 8 pg/kg appears to be one of the lowest among known flavor compounds. On the basis of the peak areas of yogurt and milk aromagrams, some compounds seem to be already present in milk, others appear to be generated by the strains. Keywords: Yogurt aroma; headspace; GC−olfactometry; impact-compound; composition
Prevention of thinned wafer deformation during thermocompression bonding and multi-die stacking supported by temporary bonding materials (2021)

Abstract Process flows for memory stacking or other heterogeneous integration schemes benefit from die bonding on a thinned silicon wafer 100 μm or less. In scenarios where a thinned device wafer contains features such as microbumps or Cu pillars, a carrier and temporary bonding material (TBM) facilitate the support of the fragile landing wafer during thermocompression bonding (TCB). The landing wafer in this case is vulnerable to deformations including loss of die planarity, Si bulging, Si or low k dielectric cracking, and damage to the underlying device wafer topography. In this paper, a dual layer system for temporary bonding is presented that maintains the integrity of a thinned device wafer during and after TCB. This is achieved with TBM materials which do not reflow at typical TCB conditions. The approach is to simulate TCB conditions which demonstrate the performance between different underlying TBM materials. A method which tracks the bond head z-axis over time during a TCB cycle is described which in turn yields information on the degree of temporary substrate deformation due to TCB force and temperature. The experiments include a worst-case scenario of multiple TCB cycles in the same position to mimic multi-die stacking. Finally, the impact of process conditions on Cu pillars with solder caps embedded in a thinned wafer bond line will be discussed.
Direct Evidence for the Interaction of the Mechanisms of Thermally Initiated and Atom Transfer Radical Polymerization (2000)

ADVERTISEMENT RETURN TO ISSUEPREVCommunication to the...Communication to the EditorNEXTDirect Evidence for the Interaction of the Mechanisms of Thermally Initiated and Atom Transfer Radical PolymerizationJames Parker, Richard G. Jones, and Simon J. HolderView Author Information Centre for Materials Research, School of Physical Sciences, University of Kent, Canterbury, Kent, CT2 7NR U.K. Cite this: Macromolecules 2000, 33, 25, 9166–9168Publication Date (Web):November 21, 2000Publication History Received21 March 2000Revised6 September 2000Published online21 November 2000Published inissue 1 December 2000https://pubs.acs.org/doi/10.1021/ma000507uhttps://doi.org/10.1021/ma000507urapid-communicationACS PublicationsCopyright © 2000 American Chemical SocietyRequest reuse permissionsArticle Views217Altmetric-Citations7LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Aromatic compounds,Hydrocarbons,Radical polymerization,Styrenes,Thermal polymerization Get e-Alerts
Taxadiene Synthase-Catalyzed Cyclization of 6-Fluorogeranylgeranyl Diphosphate to 7-Fluoroverticillenes (2005)

The mechanism of the taxadiene synthase-catalyzed cyclization of (E,E,E)-geranylgeranyl diphosphate (GGPP, 7) to taxadiene (5) is proposed to proceed through a verticillen-12-yl carbocation intermediate (8) that undergoes an 11 --> 7 proton transfer leading to formation of the C ring. The substrate analogue 6-fluoroGGPP (17) was synthesized to elucidate the stereochemistry of the putative verticillenyl intermediate. It was expected that the inductive electron-withdrawing effect of the fluoro substituent would prevent the critical proton transfer to the Delta(7) double bond and thereby derail the cyclization at the bicyclic stage. Incubation of the fluoro analogue with recombinant taxadiene synthase yielded a mixture of three major and two minor fluoro diterpenes according to GC/MS analyses. The three major products were identified as the exocyclic, endocyclic, and 4(20)-methylene 7-fluoroverticillenes, i.e., Delta(3,7,12 (18)), Delta(3,7,12), and Delta(4(20),7,11) isomers (22, 23, and 24) on the basis of (1)H NMR analyses and comparisons with the parent bicyclic diterpenes. The H1beta, H11alpha (1S,11R) configurations at the bridgehead positions of 22 were established by means of NOE experiments and CD spectra. The absolute configuration of (+)-verticillol (4) was revised after the anomalous dispersion X-ray analysis of (+)-verticillol p-iodobenzoate. Of particular note, all absolute configurations of verticillane diterpenes in the literature should be reversed. This work affords compelling evidence supporting the H11alpha (11R) stereochemistry of the verticillen-12-yl(+) ion intermediate in the taxadiene synthase-catalyzed reaction and illustrates the capability of vinyl fluoro analogues to intercept complex cyclization cascades.
Sequence-complementarity dependent co-assembly of phosphodiester-linked aromatic donor-acceptor trimers (2021)

Development of the interplay between monomer sequence and supramolecular chemistry is critical if chemistry is to recapitulate the properties of proteins and nucleic acids in the synthetic world. We have created sequenced trimers of aromatic donor/acceptor units which participate in charge-transfer interactions, linked by phosphodiesters. Each sequence displays its own characteristic self-assembly, and moreover complementary sequences interact with each other to produce new nanostructures and emergent thermochromism. This finding paves the way towards new functional nanomaterials which make bio-analogous use of sequence to tune structure.
Rapid Diagnosis of Tuberculosis with the Xpert MTB/RIF Assay in High Burden Countries: A Cost-Effectiveness Analysis (2011)

Our results suggest that Xpert is a cost-effective method of TB diagnosis, compared to a base case of smear microscopy and clinical diagnosis of smear-negative TB in low- and middle-income settings where, with its ability to substantially increase case finding, it has important potential for improving TB diagnosis and control. The extent of cost-effectiveness gain to TB programmes from deploying Xpert is primarily dependent on current TB diagnostic practices. Further work is required during scale-up to validate these findings.
Performance analysis of dynamic finite versioning for concurrent transaction and query processing (1992)

In this paper, we analyze the performance of dynamic finite versioning (DFV) schemes for concurrent transaction and query processing, where a finite number of consistent snapshots can be derived for query access. We develop analytical models based on a renewal process approximation to evaluate the performance of DFV using M ≥ 2 snapshots. The storage overhead and obsolescence faced by queries are measured. Simulation is used to validate the analytical models and to evaluate the trade-offs between various starategies for advancing snapshots when M &gt; 2. The results show that (1) the analytical models match closely with simulation; 2) both the storage overhead and obsolescence are sensitive to the snapshot-advancing strategies, especially for M &gt; 2 snapshots; and (3) generally speaking, increasing the number of snapshots demonstrates a trade-off between storage overhead and query obsolescence. For cases with skewed access or low update rates, a moderate increase in the number of snapshots beyond 2 can substantially reduce the obsolescence, while the storage overhead may increase only slightly, or even decrease in some cases. Moreover, for very low update rates, a large number of snapshots demonstrates a trade-off between storage overhead and query obsolescence. For cases with skewed access or low update rates, a moderate increase in the number of snapshots beyond 2 can substantially reduce the obsolescence, while the storage overhead may increase only slightly, or even decrease in some cases. Moreover, for very low update rates, a large number of snapshots can be used to reduce the obsolescence to almost zero without increasing the storage overhead.
XRCE's Participation to ImageCLEFphoto 2007 (2007)

Our participation to ImageCLEFphoto07, for the first time, was motivated by assessing several transmedia similarity measures that we recently designed and developed. The object of investigation consists here in some “intermediate level ” fusion approaches, where we use some principles coming from pseudo-relevance feedback and, more specifically, use transmedia pseudo-relevance feedback for enriching the mono-media representation of an object with features coming from the other media. One issue that arises when adopting such a strategy is to determine how to compute the mono-media similarity between an aggregate of objects coming from a first (pseudo-)feedback step and one single multimodal object. We propose two alternative ways of adressing this issue, that result in what we called the “transmedia document reranking ” and “complementary feedback ” methods respectively. This year, with a “lightly ” annotated corpus of images, it appears that mono-media retrieval performance is more or less equivalent for pure image and pure text content (around 20 % MAP). Using our transmedia pseudofeedback-based similarity measures
Table S1 from Delta-Like Ligand–Notch1 Signaling Is Selectively Modulated by HPV16 E6 to Promote Squamous Cell Proliferation and Correlates with Cervical Cancer Prognosis (2023)

&lt;p&gt;Clinicopathological characteristics of molecular cervical cancer subgroups (TCGA)&lt;/p&gt;
Quantifiable Assessment of SWNT Dispersion in Polymer Composites (2007)

NASA LaRC has established a new protocol for visualizing the nanomaterials in structural polymer matrix resins. Using this new technique and reconstructing the 3D distribution of the nanomaterials allows us to compare this distribution against a theoretically perfect distribution. Additional tertiary structural information can now be obtained and quantified with the electron tomography studies. These tools will be necessary to establish the structural-functional relationships between the nano and the bulk. This will also help define the critical length scales needed for functional properties. Field ready tool development and calibration can begin by using these same samples and comparing the response. i.e. gold standards of good and bad dispersion.
Involvement of a Nodofascicular Connection in Supraventricular Tachycardia with VA Dissociation (1994)

We present the case of a patient with episodes of supraventricular tachycardia and atrial dissociation that were terminated by either adenosine or verapamil. Involvement of an accessory pathway was shown by ventricular extrastimuli, elicited during His-bundle refractoriness, that interrupted the tachycardia or advanced the next His potential. The tachycardia circuit was demonstrated to be confined to the nodofascicular region based on the exclusion of surrounding tissues. Atrial activity, including that in the perinodal region, was totally dissociated during tachycardia. The lowest part of the circuit was determined to be located above the Hisian bifurcation, as multiple episodes with either a right or left bundle branch configuration during tachycardia did not modify the HH cycle. The ventricular septum summit was determined not to be involved, as no preexcitation was present during tachycardia or atrial pacing, and the right bundle branch was not part of the circuit. Radiofrequency current applied beneath the tricuspid valve at the His region successfully eliminated the nodofascicular connection with preservation of 1:1 AV conduction. The anatomical substrate underlying the abnormal connection may be either nodofasciculoventricular Mahaim fibers or a duality or dispersion of the nodo-Hisian conducting system.