gebhart/patrepeval huggingface dataset:
Phosphorylation of MUC1 by Met Modulates Interaction with p53 and MMP1 Expression (2008)

MUC1, a transmembrane mucin, is a key modulator of several signaling pathways that affect oncogenesis, motility, and cell morphology. The interaction of MUC1 cytoplasmic tail (MUC1CT) with signal transducers and its nuclear translocation and subsequent biological responses are believed to be regulated by phosphorylation status, but the precise mechanisms by which this occurs remain poorly defined. We detected a novel association between the Met receptor tyrosine kinase and the MUC1CT. Met catalyzed phosphorylation of tyrosine at YHPM in the MUC1CT. Stimulation of S2-013.MUC1F pancreatic cancer cells with hepatocyte growth factor facilitated nuclear localization of MUC1CT, as determined by real time confocal imaging analysis. MUC1 overexpression also facilitated faster turnover of Met. Phosphorylation of MUC1CT by Met enhanced its interaction with p53, which led to suppression of AP1 transcription factor activity through interactions at the MMP1 promoter, ultimately leading to reduced transcription of MMP1. This correlated with a decrease in hepatocyte growth factor-induced invasiveness when MUC1 was overexpressed. The results demonstrate that MUC1 modulates Met-mediated oncogenic signaling in cancer.
Real-time glomerular filtration rate: improving sensitivity, accuracy and prognostic value in acute kidney injury (2020)

The clinical utility of rapid GFR measurements in AKI patients remains unknown as these disruptive technologies have not been tested in studies exploring clinical outcomes. However, these approaches have the potential to improve our understanding of AKI and clinical care. This overdue technology has the potential to individualize patient care and foster therapeutic success in AKI.
Active transmission control based on photonic-crystal MOS capacitor (2007)

Silicon nanophotonics has recently attracted great attention since it offers an opportunity for low cost opto-electronic solutions based on silicon complementary metal oxide semiconductor (MOS) technology. Photonic crystal (PhC) structures with slow photon effect are expected to play a key role in future large-scale ultra-compact photonic integrated circuits. A novel vertical-MOS-capacitor-based silicon PhC waveguide structure was proposed to achieve active transmission control via the free carrier plasma dispersion effect. We designed and fabricated a single-arm PhC waveguide with MOS gate defect using silicon-on-insulator (SOI) substrate and demonstrated that a defect mode was present in the infrared region. Plane wave expansion (PWE) method based simulation indicated that high group index of the fabricated PhC waveguide could be achieved near the transmission band edge. Further investigation demonstrated that such PhC MOS capacitor would be a good candidate to realize ultra-compact transmission control.
ChemInform Abstract: Synthesis and Synthetic Application of α‐Formylvinylphosphonates. Facile Synthesis of Phosphono‐Substituted Heterocyclic Compounds. (2000)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Copper(ii) cyclam-based complexes for radiopharmaceutical applications: synthesis and structural analysis (2007)

Two molecular structures of the copper(II) complex, Cu(H(2)TETA), have been determined by X-ray crystallography. The Jahn-Teller distortion differs between the two structures; occurring either along the axis of the pendant acetate arms or across the macrocyclic ring. An analysis of deposited data from over one hundred copper(II) cyclam X-ray structures in the Cambridge Structural Database (CSD) reveals that Jahn-Teller distortion across the ring is highly unusual for such compounds in the solid state. Novel chelators based on the piperazino/side-bridged cyclam have been prepared and copper(II) complexes formed. The single crystal X-ray structures of two copper(II) complexes, with either an ester or acid N-pendant arm, have been determined and in both cases the pendant arm is bound to the metal centre.
Business Transformation Workbench (2010)

Business transformation is a key management initiative that attempts to align people, process and technology of an enterprise more closely with its business strategy and vision (Lee, 2005). Business transformation is an essential part of the competitive business cycle. Existing consulting methods and tools do not address issues such as scalability of methodology, data and knowledge management, method enforcement, asset reuse and governance, consolidated views of upstream and downstream analyses well, to name a few. This paper presents Business Transformation Workbench which is a practitioner’s tool for business transformation addressing these problems. It implements a methodical approach that was devised to analyze business transformation opportunities and make business cases for transformation initiatives and thereby provides decision-support to the consultants. The Business Transformation Workbench builds on a component-based model of a business and offers a consolidated view into clients’ operations, organization, staffing, processes and IT. It provides an intuitive way to evaluate and understand various opportunities in staff and IT consolidation and process standardization. It embodies structured analytical models, both qualitative and quantitative, to enhance the consultants’ practices. The Business Transformation Workbench has been instantiated with data from finance management domain and applied to address a client situation as a case study. An alpha testing of the tool was conducted with about dozen practitioners. The feedback has been encouraging. 90% of the consultants who tested the BT Workbench tool felt that the tool would help them do a better job during a client engagement. The tool is currently being piloted with customer engagements in a large IT consulting organization.
The Human Cytomegalovirus UL116 Gene Encodes an Envelope Glycoprotein Forming a Complex with gH Independently from gL (2016)

HCMV infection can cause severe disease in immunocompromised adults and infants infected in utero The dissection of the HCMV entry machinery is important to understand the mechanism of viral infection and to identify new vaccine antigens. The gH/gL/gO and gH/gL/UL128/UL130/UL131 (Pentamer) complexes play a key role in HCMV cell entry and tropism. Both complexes are formed by an invariant gH/gL scaffold on which the other subunits assemble. Here, we show that the UL116 gene product is expressed in infected cells and forms a heterodimer with gH. The gH/UL116 complex is carried on the infectious virions, although in smaller amounts than gH/gL complexes. No gH/UL116/gL ternary complex formed in transfected cells, suggesting that the gH/UL116 complex is independent from gL. This new gH-based gL-free complex represents a potential target for a protective HCMV vaccine and opens new perspectives on the comprehension of the HCMV cell entry mechanism and tropism.
The effect of the T7 and Escherichia coli DNA-binding proteins at the replication fork of bacteriophage T7. (1988)

In this paper we compare the effect of single-stranded DNA-binding proteins of bacteriophage T7 (gene 2.5 protein) and of Escherichia coli (SSB) at the T7 replication fork. The T7 gene 4 protein acts processively as helicase to promote leading strand synthesis and distributively as primase to initiate lagging strand synthesis by T7 DNA polymerase. On a nicked double-stranded template, the formation of a replication fork requires partial strand displacement so that gene 4 protein may bind to the displaced strand and unwind the helix catalytically. Both the T7 gene 2.5 protein and E. coli SSB act stoichiometrically to promote this initial strand displacement step. Once initiated, processive leading strand synthesis is not greatly stimulated by the single-stranded DNA-binding proteins. However, the T7 gene 2.5 protein, but not E. coli SSB, increases the frequency of initiation of lagging strand synthesis by greater than 10-fold. The results suggest a specific interaction of the T7 gene 2.5 protein with the T7 replication apparatus.
Phase Behavior and Microstructure in Aqueous Mixtures of Cationic and Anionic Surfactants (2004)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Power balance control and dimensioning of a hybrid off-grid energy system for a Nordic climate townhouse (2023)

This paper investigates conversion of a Nordic oil-heated townhouse into carbon-neutral by different energy efficiency (EE) improvements and an off-grid system including solar photovoltaics (PV), wind power, and battery and hydrogen energy storage systems (BESS and HESS). A heat-pump-based heating system including waste heat recovery (WHR) from the HESS and an off-grid electrical system are dimensioned for the building by applying models developed in MATLAB and Microsoft Excel to study the life cycle costs (LCC). The work uses a measured electrical load profile, and the heat generation of the new heating system and the power generation are simulated by commercial software. It is shown that the EE improvements and WHR from the HESS have a positive effect on the dimensioning of the off-grid system, and the LCC can be reduced by up to €2 million. With the EE improvements and WHR, the component dimensioning can be reduced by 22%–41% and 13%–51% on average, respectively. WHR can cover up to 57% of the building's annual heat demand, and full-power dimensioning of the heat pump is not reasonable when WHR is applied. Wind power was found to be very relevant in the Nordic conditions, reducing the LCC by 32%.
Membrane Bioreactor Life-Cycle Cost Assessment Simulation: Digital Game-Based Learning (2010)

Membrane Bioreactor Life-Cycle Cost Assessment Simulation: Digital Game-Based LearningWEFTEC.09 preconference workshops included a new approach to training in the wastewater industry entitled Membrane Bioreactor (MBR) Life-Cycle Cost Assessment (LCA) Simulation: Digital Game-Based Learning. The workshop provided an interactive format for teams of participants to compare the design and operation of alternative MBR configurations. This paper presents a description of the workshop,...Author(s)Dave KinnearIngmar NopensMarie-Laure PellegrinThomas MaereDaniel NolascoRichard GlassenGuclu InselYouri AmerlinkErika BaileyEvangelia BeliaImre TakacsDwight HouwelingPaul CauseyRick GiardinaSudhir MurthyPeter VanrolleghemJames BarnardHenryk MelcerPaul KrauthRod ReardonJ.B NeethlingSourceProceedings of the Water Environment FederationSubjectSession 62: Developments in Process DesignDocument typeConference PaperPublisherWater Environment FederationPrint publication date Jan, 2010ISSN1938-6478SICI1938-6478(20100101)2010:12L.4336;1-DOI10.2175/193864710798182727Volume / Issue2010 / 12Content sourceWEFTECFirst / last page(s)4336 - 4345Copyright2010Word count81Subject keywordsDigital Game-Based LearningMembrane BioreactorNutrient RemovalTraining
Data from NVP-BEZ235, a Dual PI3K/mTOR Inhibitor, Prevents PI3K Signaling and Inhibits the Growth of Cancer Cells with Activating PI3K Mutations (2023)

&lt;div&gt;Abstract&lt;p&gt;Phosphatidylinositol-3-kinase (PI3K) pathway deregulation is a common event in human cancer, either through inactivation of the tumor suppressor phosphatase and tensin homologue deleted from chromosome 10 or activating mutations of p110-α. These hotspot mutations result in oncogenic activity of the enzyme and contribute to therapeutic resistance to the anti-HER2 antibody trastuzumab. The PI3K pathway is, therefore, an attractive target for cancer therapy. We have studied NVP-BEZ235, a dual inhibitor of the PI3K and the downstream mammalian target of rapamycin (mTOR). NVP-BEZ235 inhibited the activation of the downstream effectors Akt, S6 ribosomal protein, and 4EBP1 in breast cancer cells. The antiproliferative activity of NVP-BEZ235 was superior to the allosteric selective mTOR complex inhibitor everolimus in a panel of 21 cancer cell lines of different origin and mutation status. The described Akt activation due to mTOR inhibition was prevented by higher doses of NVP-BEZ235. NVP-BEZ235 reversed the hyperactivation of the PI3K/mTOR pathway caused by the oncogenic mutations of p110-α, E545K, and H1047R, and inhibited the proliferation of HER2-amplified BT474 cells exogenously expressing these mutations that render them resistant to trastuzumab. In trastuzumab-resistant BT474 H1047R breast cancer xenografts, NVP-BEZ235 inhibited PI3K signaling and had potent antitumor activity. In treated animals, there was complete inhibition of PI3K signaling in the skin at pharmacologically active doses, suggesting that skin may serve as surrogate tissue for pharmacodynamic studies. In summary, NVP-BEZ235 inhibits the PI3K/mTOR axis and results in antiproliferative and antitumoral activity in cancer cells with both wild-type and mutated p110-α. [Cancer Res 2008;68(19):8022–30]&lt;/p&gt;&lt;/div&gt;
Biomaterials Directed In Vivo Osteogenic Differentiation of Mesenchymal Cells Derived from Human Embryonic Stem Cells (2013)

Spontaneous differentiation of human embryonic stem cells (hESCs) is generally inefficient and leads to a heterogeneous population of differentiated and undifferentiated cells, limiting the potential use of hESCs for cell-based therapy and studies of specific differentiation programs. Here, we demonstrate biomaterial-dependent commitment of a mesenchymal cell population derived from hESCs toward the osteogenic lineage in vivo. In skeletal development, bone formation from condensing mesenchymal cells involves two distinct pathways: endochondral and intramembraneous bone formation. In this study, we demonstrate that the hESC-derived mesenchymal cells differentiate and regenerate in vivo bone tissues through two different pathways depending upon the local cues present in a scaffold microenvironment. Hydroxyapatite (HA) was incorporated into biodegradable poly(lactic-co-glycolic acid)/poly(l-lactic acid) (PLGA/PLLA) scaffolds to enhance bone formation. The HA microenvironment stabilized the β-catenin and upregulated Runx2, resulting in faster bone formation through intramembraneous ossification. hESC-derived mesenchymal cells seeded on the PLGA/PLLA scaffold without HA, however, showed minimal levels Runx2, and differentiated via endochondral ossification, as evidenced by formation of cartilaginous tissue, followed by calcification and increased blood vessel invasion. These results indicate that the ossification mechanisms of the hESC-derived mesenchymal stem cells can be regulated by the scaffold-mediated microenvironments, and bone tissue can be formed.
Study of high temperature stable fiber Bragg gratings fabricated in H 2 loaded and unloaded SMF-28 fiber (2009)

High reflectivity fiber Bragg gratings are written with ultrafast infrared radiation in H<sub>2</sub> loaded SMF-28 fiber that retain in excess of 60 percent of their index change at 1000 &deg;C using writing intensities below the damage threshold of the fiber. The reflectivity after annealing (&gt;-15 dB transmission loss at the Bragg resonance) and index modulation (1&times;10<sup>-3</sup>) in these devices is significantly higher than what can be achieved with UV devices such as Type II, Type IIA and chemical composition gratings. In this study the stability of these devices is compared to that of gratings fabricated in unloaded SMF-28 fiber.
An Improved Prostate Specific Membrane Antigen Targeting Agent for Prostate Cancer Imaging and Therapy (2018)

2 Introduction: Several radioligands targeting prostate-specific membrane antigen (PSMA) have been clinically introduced as a new class of radiotheranostics for imaging and treating prostate cancer. Among them, methyl cysteine-glutamate urea (MCG) has been successfully labeled with both fluorescent dyes and radioisotopes for prostate cancer imaging. The aim of this study was to conjugate MCG with an albumin binding moiety to further improve tumor delivery and increase therapeutic index. Materials and Methods: MCG was conjugated with an Evans blue (EB) derivative for albumin binding and a DOTA molecule as a metal chelator. PSMA positive PC3-PIP and PSMA negative PC3 cells were used for both in vitro and in vivo studies. Longitudinal PET imaging was performed at 1, 4, 24, and 48 h post injection to evaluate biodistribution and tumor uptake of 86Y-DOTA-EB-MCG. DOTA-EB-MCG was also labeled with 90Y for radioligand therapy (RLT). Results: Based on region of analysis (ROI) of 86Y-DOTA-EB-MCG PET images, the tracer uptake in PSMA+ PC-3 PIP tumors increased from 18.74 ± 1.28 %ID/g at 1 h p.i. to the peak of 39.47 ± 3.04 %ID/g at 24 h p.i., and was still as high as 34.05 ± 4.06 % ID/g at 48 h p.i.. Administration of 3.7 MBq of 90Y-DOTA-EB-MCG resulted in significant PC3-PIP tumor growth inhibition. When a 7.4 MBq of 90Y-DOTA-EB-MCG single dose was used, the tumors were completely eradicated. As a comparison, 7.4 MBq of 90Y-DOTA-MCG only showed marginal delay of tumor growth. Conclusion: Modification of MCG with the EB derivative resulted in a highly efficient prostate cancer targeting agent (EB-MCG), which showed great potential in prostate cancer treatment after being labeled with therapeutic radioisotopes.
Recommended Practices for Characterization and Documentation of Active Flow Control Actuators (2021)

View Video Presentation: https://doi.org/10.2514/6.2021-2002.vid This document provides a reference for recommended practices for characterization and documentation of active flow control actuators. The work is motivated by the need to develop a uniform, complete and objective reporting practice in the literature. The recommendations discussed herein should be tractable for experimental, computational and theoretical practitioners of active flow control. This document is not intended to impose practices of how to conduct research. Rather, it is purely a guide for reporting, such that objective and consistent evaluations/comparisons can be obtained. Some general reporting comments are provided followed by specific recommendations for both nonzero and zero net mass flux devices (both synthetic jets and plasma). Specific examples for all actuator types are not feasible, but the information and parameters presented herein are sufficiently complete to allow proper reporting for existing and new techniques that may arise in the future. It is acknowledged that reporting of every parameter discussed herein may be impractical or impossible for some actuators and researchers. However, recommendations are provided at various levels of fidelity to ensure that some measure of reporting is attainable. Finally, some comments about performance evaluation are provided, without these being the primary focus of the document. It should be noted that citations in this document are limited in an effort to present unbiased information that is based on general engineering knowledge. Note that the official best practice document is currently moving through the publication process with AIAA and will serve as the official reference once approved.
XML Schema Integration to Facilitate E-Commerce (2011)

XML has become the de facto standard for Information Exchange protocol for e-commerce and many workgroup applications such as Enterprise Resource Planning (ERP). The availability of large amounts of heterogeneous distributed web data necessitates the integration of XML data from multiple XML sources for many reasons. Currently, there are many e-commerce companies, which sell similar products but represent them using different XML schemas with possibly different ontologies. When any two such companies merge, there is a need for a uniform schema integration methodology. In some applications like comparison-shopping, there is a need for an illusionary centralized homogeneous information system. In this chapter, we propose an XML Schema integration methodology. We define an object-oriented data model called XSDM (XML Schema Data Model) and present a graphical representation of XML Schema for the purpose of schema integration. We use a three-layered architecture for XML Schema integration, with each layer presenting an integrated view of the concepts that characterize the layer below. The three layers included are namely pre-integration, comparison and integration. During pre-integration, an analysis of the schemas to be integrated occurs. During the comparison phase of integration, correspondences as well as conflicts between elements are identified. During the integration phase, restructuring and merging of the initial schemas takes place to obtain the global schema. We define integration policies for integrating element definitions as well as their data types and attributes. The policies are also applicable in integrating DTD schemas with other DTD/XML Schemas.Request access from your librarian to read this chapter's full text.
Effect of Hydroxylated Compounds on Properties and Emission of Palm Biodiesel (2007)

The Malaysian Palm Oil Board (MPOB) attempted the search for a viable alternative fuel leading to a possible substitution to the conventional diesel since 1982, using palm oil derived fuel. Numerous studies have shown that most exhaust emission encountered with conventional diesel fuel are reduced with biodiesel,with the exception of nitrogen oxides (NOx). This paper is reported on the effect of hydroxylated compounds on the exhaust gas emission and fuel properties of palm biodiesel. Fuel properties tested are viscosity, specific gravity, energy content, pour point and flash point. No significant decrease in the energy content when 5% (wt/vol) of hydroxylated compounds were added into palm biodiesel, however the viscosity is increased about 20%. There is a correlation between hydroxyl value of the compound and the reduction of the flame temperature. Higher hydroxyl value resulted in more significant reduction of flame temperature. The engine test run using the most promising compound showed a decrease in the NOx emission.
Thermally Activated Delayed Fluorescence Behavior Investigation in the Different Polarity Acceptor and Donor Molecules (2016)

To investigate the influence of intramolecular charge transfer (CT) characteristics, rigidity, and polarity of a molecule on photophysical properties, we designed and synthesized two types of thermally activated delayed fluorescence (TADF) emitters with malononitrile and acrylonitrile moieties as electron accepting units. Their photophysical properties such as singlet–triplet energy split (ΔEST), photoluminescence quantum yields (PLQYs), and electronic structures were theoretically and experimentally evaluated. Among the synthesized materials, the emitters with an acrylonitrile moiety as an acceptor and phenoxazine, dimethylacridine, and tert-butylcarbazole as a donor revealed small ΔEST values, good PLQYs, and efficient TADF performances. In contrast, the malononitrile derivatives demonstrated high ΔEST values, very low PLQYs, and relatively poor TADF performances even though they have strong intramolecular CT characteristics and high polarity. We found that high molecular polarity and strong intramolecular CT characteristics are not essential factors for attaining good TADF performances over molecular rigidity.