gebhart/patrepeval huggingface dataset:
The putative nuclear localization signal of the human RAD52 protein is a potential sumoylation site (2010)

RAD52, a key factor in homologous recombination (HR), plays important roles in both RAD51-dependent and -independent HR pathways. Several studies have suggested a link between the functional regulation of RAD52 and the protein modification by a small ubiquitin-like modifier (SUMO). However, the molecular mechanism underlying the regulation of RAD52 by SUMO is unknown. To begin investigating this mechanism, we identified possible target sites for sumoylation in the human RAD52 protein by preparing a RAD52-SUMO complex using an established Escherichia coli sumoylation system. Mass spectrometry and amino acid sequencing of the enzymatically digested fragments of the purified complex revealed that the putative nuclear localization signal located near the C terminus of RAD52 was sumoylated. Biochemical studies of the RAD52-SUMO complex suggested that sumoylation at the identified site has no apparent effect on the DNA binding, D-loop formation, ssDNA annealing and RAD51-binding activities of RAD52. On the other hand, visualization of the GFP-fused RAD52 protein in the human cell that contained mutations at the identified sumoylation sites showed clear differences in the cytosolic and nuclear distributions of the protein. These results suggest the possibility of sumoylation playing an important role in the nuclear transport of RAD52.
Advanced 3D eWLB-PoP (embedded Wafer Level Ball Grid Array - package on package) technology (2015)

The advancement of silicon scaling to 14/16 nanometer (nm) in support of higher performance, higher bandwidth and lower power consumption in portable and mobile devices is pushing the boundaries of emerging packaging technologies to smaller fan-out packaging designs with finer line/spacing as well as improved electrical performance and passive embedded technology capabilities. Advanced embedded Wafer Level Ball Grid Array (eWLB) technology provides a versatile platform for the semiconductor industry's technology evolution from single or multi-die 2D package designs to 2.5D interposers and 3D System-in-Package (SiP) configurations.
Cryptic Aspergillus nidulans Antimicrobials (2011)

Secondary metabolite (SM) production by fungi is hypothesized to provide some fitness attribute for the producing organisms. However, most SM clusters are "silent" when fungi are grown in traditional laboratory settings, and it is difficult to ascertain any function or activity of these SM cluster products. Recently, the creation of a chromatin remodeling mutant in Aspergillus nidulans induced activation of several cryptic SM gene clusters. Systematic testing of nine purified metabolites from this mutant identified an emodin derivate with efficacy against both human fungal pathogens (inhibiting both spore germination and hyphal growth) and several bacteria. The ability of catalase to diminish this antimicrobial activity implicates reactive oxygen species generation, specifically, the generation of hydrogen peroxide, as the mechanism of emodin hydroxyl activity.
Intracerebroventricular injection of interleukin 1 induces high circulating levels of interleukin 6. (1990)

IL-1 is known to have a central role in the induction of acute-phase response, and some of its activities (including induction of some acute-phase proteins) were reported to be mediated by an induction of IL-6. Administration to rats of 200 ng of human rIL-1 by intracerebroventricular injection resulted in a more marked induction of circulating IL-6 than the same dose of IL-1 administered systemically (intravenously or intraperitoneally). Induction of serum IL-6 by centrally administered IL-1 was also observed in hypophysectomized or adrenalectomized rats, suggesting that activation of the hypothalamus-pituitary-adrenal axis is not essential for this effect of IL-1. IL-6 induction was also observed after pretreatment with indomethacin, indicating that the effect was dissociated from the pyrogenic activity of IL-1. Induction of IL-6 by a central action could represent a novel pathway in IL-1-induced acute-phase response.
High Speed Arbitrary Waveform Generation and Processing using a Photonic Digital-to-Analog Converter (2007)

In this paper we report on our recent progress in using photonic digital-analog converters for arbitrary waveform generation. Optical arbitrary waveforms are generated using a time-domain approach based on an interfere metric modulator device.
Multi-Wavelength Message Routing in a Non-Blocking Four-Port Bidirectional Switch Fabric for Silicon Photonic Networks-on-Chip (2009)

A non-blocking four-port bidirectional multi-wavelength message router for use in 2D mesh or torus photonic networks-on-chip is fully characterized with bit-error-rate measurements and eye diagrams using three parallel 10-Gb/s wavelength channels.
13.5-inch Quadra-FHD flexible AMOLED with crystalline oxide FET (2012)

This paper reports on our 13.5-inch top-emission flexible AMOLED in which CAAC-IGZO FETs are used as driver elements. We have realized a top-emission flexible AMOLED by using a technique of forming FET elements featuring low off-state current and high reliability and a passivation film with high barrier property over a glass substrate, separating the FET portion from the glass substrate by physical force, and transferring the FET portion to a plastic substrate.
The risk of hemophagocytic lymphohistiocytosis in Hermansky-Pudlak syndrome type 2 (2013)

Genetic disorders of lymphocyte cytotoxicity predispose patients to hemophagocytic lymphohistiocytosis (HLH). Reduced lymphocyte cytotoxicity has been demonstrated in Hermansky-Pudlak syndrome type 2 (HPS2), but only a single patient was reported who developed HLH. Because that patient also carried a potentially contributing heterozygous RAB27A mutation, the risk for HLH in HPS2 remains unclear. We analyzed susceptibility to HLH in the pearl mouse model of HPS2. After infection with lymphocytic choriomeningitis virus, pearl mice developed all key features of HLH, linked to impaired virus control caused by a moderate defect in CTL cytotoxicity in vivo. However, in contrast to perforin-deficient mice, the disease was transient, and all mice fully recovered and controlled the infection. An additional heterozygous Rab27a mutation did not aggravate the cytotoxicity defect or disease parameters. In the largest survey of 22 HPS2 patients covering 234 patient years, we identified only 1 additional patient with HLH and 2 with incomplete transient HLH-like episodes, although cytotoxicity or degranulation was impaired in all 16 patients tested. HPS2 confers a risk for HLH that is lower than in Griscelli or Chediak-Higashi syndrome, probably because of a milder defect in cytotoxicity. Preemptive hematopoietic stem cell transplantation does not appear justified in HPS2.
The international debate on new generation of therapeutic cancer vaccinesaffordable to the masses (2020)

Immunotherapy is now accepted as a new treatment modality for cancer. US FDA approved checkpoint blockage, anti-
CTLA4 and anti-PD1/L1, monoclonal antibody drugs and autologous CAR-T cells are the first generation of drugs with an
immunemediated anti-tumor mechanism. These immunotherapy drugs have demonstrated ability to control metastatic
disease and hematological malignancies. However, these first generation immunotherapy drugs work in only a limited
number of indications and in only a minority subset of patients within these limited indications, particularly the small
subset with tumors that express high mutational load status.
Faculty Opinions recommendation of A proof of concept for structure-based vaccine design targeting RSV in humans. (2019)

Technologies that define the atomic-level structure of neutralization-sensitive epitopes on viral surface proteins are transforming vaccinology and guiding new vaccine development approaches. Previously, iterative rounds of protein engineering were performed to preserve the prefusion conformation of the respiratory syncytial virus (RSV) fusion (F) glycoprotein, resulting in a stabilized subunit vaccine candidate (DS-Cav1), which showed promising results in mice and macaques. Here, phase I human immunogenicity data reveal a more than 10-fold boost in neutralizing activity in serum from antibodies targeting prefusion-specific surfaces of RSV F. These findings represent a clinical proof of concept for structure-based vaccine design, suggest that development of a successful RSV vaccine will be feasible, and portend an era of precision vaccinology.Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. PMID: 31371616
Pneumococcal epidemiology among us adults hospitalized for community-acquired pneumonia (2019)

After implementation of a pneumococcal conjugate vaccination program in US children, and despite the herd protection observed in US adults, a persistent burden of PCV13-type CAP remains in this population.
Resistance to Chemotherapy: Patient Variability and Cellular Heterogeneity (2014)

The issue of resistance to targeted drug therapy is of pressing concern, as it constitutes a major barrier to progress in managing cancer. One important aspect is the role of stochasticity in determining the nature of the patient response. We examine two particular experiments. The first measured the maximal response of melanoma to targeted therapy before the resistance causes the tumor to progress. We analyze the data in the context of a Delbruck-Luria type scheme, wherein the continued growth of preexistent resistant cells are responsible for progression. We show that, aside from a finite fraction of resistant cell-free patients, the maximal response in such a scenario would be quite uniform. To achieve the measured variability, one is necessarily led to assume a wide variation from patient to patient of the sensitive cells' response to the therapy. The second experiment is an in vitro system of multiple myeloma cells. When subject to a spatial gradient of a chemotherapeutic agent, the cells in the middle of the system acquire resistance on a rapid (two-week) timescale. This finding points to the potential important role of cell-to-cell differences, due to differing local environments, in addition to the patient-to-patient differences encountered in the first part. See all articles in this Cancer Research section, "Physics in Cancer Research."
CARLOC (2015)

Precise positioning of an automobile to within lane-level precision can enable better navigation and context-awareness. However, GPS by itself cannot provide such precision in obstructed urban environments. In this paper, we present a system called CARLOC for lane-level positioning of automobiles. CARLOC uses three key ideas in concert to improve positioning accuracy: it uses digital maps to match the vehicle to known road segments; it uses vehicular sensors to obtain odometry and bearing information; and it uses crowd-sourced location of estimates of roadway landmarks that can be detected by sensors available in modern vehicles. CARLOC unifies these ideas in a probabilistic position estimation framework, widely used in robotics, called the sequential Monte Carlo method. Through extensive experiments on a real vehicle, we show that CARLOC achieves sub-meter positioning accuracy in an obstructed urban setting, an order-of-magnitude improvement over a high-end GPS device.
Matriptase activates stromelysin (MMP‐3) and promotes tumor growth and angiogenesis (2006)

Matriptase/MT‐SP1, a type II membrane serine protease widely expressed in normal epithelial cells and human carcinoma cells, is thought to be involved in cancer progression. To clarify this possibility, we overexpressed exogenous matriptase in the human stomach cancer cell line AZ521. In vitro , the matriptase transfectant (Mat‐AZ521) and the control transfectant (Mock‐AZ521) showed a similar growth rate, although the saturation cell density was significantly higher with the Mat‐AZ521. When implanted into nude mice subcutaneously or intraperitoneally, Mat‐AZ521 cells grew faster and produced much larger solid tumors than Mock‐AZ521 cells. The overexpression of matriptase in AZ521 cells shortened the survival time of tumor‐bearing mice. Histological analysis showed that both the number and the size of blood vessels in tumor tissues were significantly higher in the Mat‐AZ521 tumors than the Mock‐AZ521 ones. Moreover, it was found that purified matriptase activated one of the important matrix metalloproteinases, stromelysin (MMP‐3). These results suggest the possibility that the matriptase‐dependent activation of MMP‐3, as well as the direct activity of matriptase, promotes tumor growth and angiogenesis by enhancing extracellular matrix degradation in tumor cell microenvironments. ( Cancer Sci 2006; 97: 1327–1334)
<title>Optical 3D-coordinate measuring system using structured light</title> (1996)

The paper is aimed at the description of an optical shape measuring technique based on a consistent principle using fringe projection technique. We demonstrate a real 3D- coordinate measuring system where the sale of coordinates is given only by the illumination-structures. This method has the advantages that the aberration of the observing system and the depth-dependent imaging scale have no influence on the measuring accuracy and, moreover, the measurements are independent of the position of the camera with respect to the object under test. Furthermore, it is shown that the influence of specular effects of the surface on the measuring result can be eliminated. Moreover, we developed a very simple algorithm to calibrate the measuring system. The measuring examples show that a measuring accuracy of 10<SUP>-4</SUP> (i.e. 10 micrometers ) within an object volume of 100 X 100 X 70 mm<SUP>3</SUP> is achievable. Furthermore, it is demonstrated that the set of coordinate values can be processed in CNC- and CAD-systems.
ChemInform Abstract: Synthesis of Monoterpenic α‐Amino Ketones. (1996)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Injectable Flexible Subcutaneous Electrode Array Technology for Electrocardiogram Monitoring Device (2019)

Implantable cardiac monitors have undergone considerable miniaturization. However, they continue to be associated with complications such as infection, bleeding/bruising, and device extrusion or migration. In this paper, we demonstrate the feasibility of using a small, flexible, injectable, subcutaneous microelectrode-based device to record electrocardiograms (ECGs). We describe the fabrication process and demonstrate the ease of insertion of the injectable ECG device in vivo swine model. We also demonstrate our device’s high-density channel microelectrode array’s ability to detect the P, R, and T waves. The amplitude of these waves showed excellent correlation with distance of the bipolar electrodes used to detect them. Given the success of our initial studies, this device has the potential to improve the safety profile of implantable cardiac monitors and simplify the implantation procedure to allow for placement in a primary care setting.
Cyclooctatetrene derivatives as electron transporters and hole blocker in organic LEDs (2001)

In this paper, we report that novel tetraphenyl- tetraalkynyl-COTs and octaaryl-COTs can be used as electron transport and hole blocker layer in organic LEDs. These materials have the similar reduction potentials compared to traditional electron transporters, such as Alq<SUB>3</SUB>. The energy gaps for all of the COT derivatives studied here are very wide (&gt; 3 eV), making it possible to fabricate blue emitting OLEDs with these materials. We have fabricated devices of the structure ITO/NPD400 angstrom/COT-Me 400 angstrom/Mg-Ag. (ITO equals indium-tin-oxide, NPD equals N,N'-diphenyl-N,N'-dinapthylbenzidine). The external quantum yield reaches 0.15% and the emission comes from NPD ((lambda) <SUB>max</SUB> equals 436 nm). When 1% perylene (by weight) is doped into the NPD, the (lambda) <SUB>max</SUB> of emission shifts to the 454 nm with the external QE reaches 0.60%. It indicates the efficient energy transfer from NPD to perylene. Changing the COT layer thickness from 400 Angstrom to 200 Angstrom, the emission is still from the NPD layer, as seen before. However, the I-V curve and turn-on voltage both shift to lower potentials by ca 2 - 3 V with the QE drops to 0.06%. The device QE can be increased by either using thin Alq layer or replacing Mg:Ag with LiF/Al electrode. Finally, the devices using COTs as hole blocker are fabricated and results are discussed.
Parametric Delayed Output Feedback Control for Versatile Human-Exoskeleton Interactions During Walking and Running (2023)

This letter presents a parametric delayed output feedback controller for various types of human-exoskeleton interactions during walking or running gait. The parametric delayed-feedback controller operates reliably during various gait pattern changes, utilizing three adjustable control parameters: smoothing factor, time delay, and feedback gain. We show that various human-exoskeleton interaction torques can be generated using these three parameters, such as assistance with running or walking and different types of resistances. Feasibility tests were performed to demonstrate the efficacy of the proposed interaction controller. Hip exoskeleton assistance reduces the metabolic cost by 12.3% during running at 7 km/h compared with running without the exoskeleton. The generalizability of the controller performance was also demonstrated through various gait speed tests. Furthermore, we measured the electromyography signal data and the human lower limb joint kinematics to examine the feasibility of providing different gait resistances using different time delays. The results show that resistance with a delay of 0.3 s (Late mode) instead of a delay of 0.1 s (Early mode) is possible for walking resistance training, focusing on the hip flexors compared to the zero-torque mode.
The Northrop Grumman Compact Infrared FEL (CIRFEL) (2002)

As part of Northrop Grumman's ongoing research in particle accelerators, we have designed and are commissioning a Compact Infrared Free-Electron Laser (CIRFEL) for the study of high-brightness electron beams and free-electron lasers. Besides serving as a tool for FEL development, the CIRFEL laboratory, located at Princeton University, will be used in experiments ranging from basic FEL physics and biophysics to chemistry, materials science and medicine. The CIRFEL is to lase initially in the 10 - 20 /spl mu/m range. The pulse format of this FEL is a train of micropulses, 5 - 10 psec is duration, at a repetition rate of 142.8 MHz. The micropulse energy is in excess of 100 /spl mu/J. The micropulses comprise a macropulse lasting approximately 10 /spl mu/sec. The macropulse repetition rate is 10 Hz, thus the average power of the FEL is on the order of 1.5 W.
A Model for Estimating Tactile Sensation by Machine Learning Based on Vibration Information Obtained while Touching an Object (2021)

The tactile sensation is an important indicator of the added value of a product, and it is thus important to be able to evaluate this sensation quantitatively. Sensory evaluation is generally used to quantitatively evaluate the tactile sensation of an object. However, statistical evaluation of the tactile sensation requires many participants and is, thus, time-consuming and costly. Therefore, tactile sensing technology, as opposed to sensory evaluation, is attracting attention. In establishing tactile sensing technology, it is necessary to estimate the tactile sensation of an object from information obtained by a tactile sensor. In this research, we developed a tactile sensor made of two-layer silicone rubber with two strain gauges in each layer and obtained vibration information as the sensor traced an object. We then extracted features from the vibration information using deep autoencoders, following the nature of feature extraction by neural firing due to vibrations perceived within human fingers. We also conducted sensory evaluation to obtain tactile scores for different words from participants. We finally developed a tactile sensation estimation model for each of the seven samples and evaluated the accuracy of estimating the tactile sensation of unknown samples. We demonstrated that the developed model can properly estimate the tactile sensation for at least four of the seven samples.
Rapid Isolation of Staphylococcus aureus Pathogens from Infected Clinical Samples Using Magnetic Beads Coated with Fc-Mannose Binding Lectin (2016)

Here we describe how Staphylococcus aureus bacteria can be rapidly isolated from clinical samples of articular fluid and synovial tissue using magnetic beads coated with the engineered chimeric human opsonin protein, Fc-mannose-binding lectin (FcMBL). The FcMBL-beads were used to capture and magnetically remove bacteria from purified cultures of 12 S. aureus strains, and from 8 articular fluid samples and 4 synovial tissue samples collected from patients with osteoarthritis or periprosthetic infections previously documented by positive S. aureus cultures. While the capture efficiency was high (85%) with purified S. aureus strains grown in vitro, direct FcMBL-bead capture from the clinical samples was initially disappointing (< 5% efficiency). Further analysis revealed that inhibition of FcMBL binding was due to coating of the bacteria by immunoglobulins and immune cells that masked FcMBL binding sites, and to the high viscosity of these complex biological samples. Importantly, capture of pathogens using the FcMBL-beads was increased to 76% efficiency by pretreating clinical specimens with hypotonic washes, hyaluronidase and a protease cocktail. Using this approach, S. aureus bacteria could be isolated from infected osteoarthritic tissues within 2 hours after sample collection. This FcMBL-enabled magnetic method for rapid capture and concentration of pathogens from clinical samples could be integrated upstream of current processes used in clinical microbiology laboratories to identify pathogens and perform antibiotic sensitivity testing when bacterial culture is not possible or before colonies can be detected.
Message from technical program co-chairs (2013)

The 19th IEEE Workshop on Local and Metropolitan Area Networks features invited papers in addition to regular track papers from both industry and academia. We received 32 submissions for regular papers. After a careful review process, the technical program committee (TPC) selected 10 regular papers and 4 short papers for presentation in the workshop program, resulting in 31% and 44% acceptance rates, respectively. Each regular submission received at least three reviews. We also received 8 invited paper submissions from experts performing research in areas related to the theme of this year's workshop: Seamless Services. We are very pleased to have the invited papers by Peng He, Thomas Mager, Paris Charalampou, Jean-Michel Dricot, Vanniarajan Chellappan, Dan Li, Gianluca Reali, Patrick Thiran and their co-authors. Each invited paper submission received at least two reviews.
Supplementary Table from Improved T-cell Immunity Following Neoadjuvant Chemotherapy in Ovarian Cancer (2023)

Supplementary Table from Improved T-cell Immunity Following Neoadjuvant Chemotherapy in Ovarian Cancer