Morphometric Analysis of Smooth Muscle in the Exstrophy-Epispadias Complex (1996)

No AccessJournal of UrologyExstrophy/Epispadias1 Aug 1996Morphometric Analysis of Smooth Muscle in the Exstrophy-Epispadias Complex Alberto Lais, Nazareno Paolocci, Fabio Ferro, Cesare Bosman, Renata Boldrini, and Paolo Caione Alberto LaisAlberto Lais , Nazareno PaolocciNazareno Paolocci , Fabio FerroFabio Ferro , Cesare BosmanCesare Bosman , Renata BoldriniRenata Boldrini , and Paolo CaionePaolo Caione View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)65827-0AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: In bladder exstrophy and to a lesser extent in epispadias th e muscular tissue in the bladder wall may often be replaced by collagen. The aim of our study was to assess the relative changes of smooth muscle versus connective tissue in patients with the exstrophy-epispadias complex. Materials and Methods: Nine full thickness detrusor biopsies from children with the exstrophy-epispadias complex were analyzed. Biopsies were stained with the Masson trichrome method to differentiate muscular tissue from collagen. At X50 magnification using image software for computerized morphometry the areas of smooth muscle and collagen were measured and expressed as a percent. As controls, detrusor biopsies from 8 children with unilateral obstructive megaureter or ectopic ureter were analyzed using the same method. Results: The average rates of smooth muscle in children with the exstrophy-epispadias complex were 7, 19.3 and 31.5 percent, respectively, in newborns, and before and after bladder neck reconstruction. In controls the rate average 56.5 percent. Conclusions: The ratio of smooth muscle-to-connective tissue increases from the newborn period to puberty in the exstrophy-epispadias complex. After staged reconstruction is completed this ratio remains below normal, although it is increased. Such a change may represent a histological marker of the inadequate volume increase of some of these bladders. References 1 : Quantitative morphometry of the adult human bladder. J. Urol., part 21992; 148: 414. Abstract, Google Scholar 2 : Bladder wall abnormalities in myelodysplastic bladders: a computer assisted morphometric analysis. J. Urol.1991; 145: 1024. Link, Google Scholar 3 Caione, P., Lais, A., Rossi, L., De Gennaro, M. and Capozza, N.: The “never functioning” bladder: pitfalls of vesico-ureteral reimplants. Read at annual meeting of European Society of Paediatric Urology, Goteborg, Sweden, April 28-30, 1994. Google Scholar 4 : Bladder evaluation in pediatric patients before undiversion in previously diverted urinary tracts. J. Urol.1977; 118: 443. Link, Google Scholar 5 : Effect of disuse and restoration of function on vesical capacity. J. Urol.1969; 101: 700. Link, Google Scholar 6 : Impairment and restoration of rat urinary bladder responsiveness following distention. Amer. J. Physiol.1983; 244: R106. Google Scholar 7 : Supersensitivity to carbachol in the parasympathetically decentrated feline urinary bladder. J. Urol.1984; 131: 562. Google Scholar 8 : Disuse as a cause of supersensitivity in the rat urinary bladder. Acta Physiol. Scand.1986; 126: 429. Google Scholar 9 : Urinary diversion in myelodysplasia: criteria for selection and prediction of urodynamic evaluation. J. Urol.1980; 124: 89. Link, Google Scholar 10 : Experience with urinary undiversion in children with neurogenic bladder. J. Urol.1980; 123: 402. Link, Google Scholar 11 : Functional and pharmacological effects of ureteral diversion. J. Urol.1989; 141: 403. Link, Google Scholar 12 : The importance of a successful initial bladder closure in the surgical management of classical bladder exstrophy: analysis of 144 patients treated at the Johns Hopkins Hospital between 1975 and 1985. J. Urol.1987; 137: 258. Link, Google Scholar 13 : Bladder function and dysfunction in exstrophy and epispadias. Lancet1991; 138: 926. Google Scholar 14 : Is lamina propria responsible for normal bladder compliance?. J. Urol., part 21992; 148: 544. Abstract, Google Scholar 15 : Collagen and elastin in the normal fetal bladder. J. Urol., part 21991; 146: 524. Abstract, Google Scholar 16 : Collagen and elastin in the obstructed fetal bladder. J. Urol., part 21991; 146: 528. Abstract, Google Scholar From the Departments of Pediatric Surgery and Pathology, Bambino Gesu Children's Hospital, Rome and Department of Pathology, University of Perugia, Perugia, Italy.© 1996 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited bySuson K, Stec A, Gearhart J and Shimoda L (2012) Transforming Growth Factor-β1 Mediates Migration in Cultured Human Control and Exstrophy Bladder Smooth Muscle CellsJournal of Urology, VOL. 188, NO. 4S, (1528-1534), Online publication date: 1-Oct-2012.Collado A, Batista E, Gelabert-Más A, Corominas J, Arañó P and Villavicencio H (2018) Detrusor Quantitative Morphometry in Obstructed Males and ControlsJournal of Urology, VOL. 176, NO. 6, (2722-2728), Online publication date: 1-Dec-2006.CAIONE P, CAPOZZA N, LAIS A and MATARAZZO E (2018) PERIURETHRAL MUSCLE COMPLEX REASSEMBLY FOR EXSTROPHY-EPISPADIAS REPAIRJournal of Urology, VOL. 164, NO. 6, (2062-2066), Online publication date: 1-Dec-2000.MATHEWS R, WILLS M, PERLMAN E and GEARHART J (2018) NEURAL INNERVATION OF THE NEWBORN EXSTROPHIC BLADDER: AN IMMUNOHISTOCHEMICAL STUDYJournal of Urology, VOL. 162, NO. 2, (506-508), Online publication date: 1-Aug-1999.LIPSKI B, YOSHINO K, YAO L, CARR M and MITCHELL M (2018) A UNIQUE NEW MODEL TO STUDY THE EFFECTS OF URINARY DIVERSION IN THE DEVELOPING RABBIT BLADDERJournal of Urology, VOL. 160, NO. 4, (1454-1458), Online publication date: 1-Oct-1998. Volume 156Issue 2SAugust 1996Page: 819-821 Advertisement Copyright & Permissions© 1996 by American Urological Association, Inc.MetricsAuthor Information Alberto Lais More articles by this author Nazareno Paolocci More articles by this author Fabio Ferro More articles by this author Cesare Bosman More articles by this author Renata Boldrini More articles by this author Paolo Caione More articles by this author Expand All Advertisement PDF downloadLoading ...