gebhart/patrepeval huggingface dataset:
Data from Oncolytic Adenoviral Delivery of an EGFR-Targeting T-cell Engager Improves Antitumor Efficacy (2023)

&lt;div&gt;Abstract&lt;p&gt;Antiviral immune responses present a major hurdle to the efficacious use of oncolytic adenoviruses as cancer treatments. Despite the existence of a highly immunosuppressive tumor environment, adenovirus-infected cells can nonetheless be efficiently cleared by infiltrating cytotoxic T lymphocytes (CTL) without compromising tumor burden. In this study, we tested the hypothesis that tumor-infiltrating T cells could be more effectively activated and redirected by oncolytic adenoviruses that were armed with bispecific T-cell–engager (BiTE) antibodies. The oncolytic adenovirus ICOVIR-15K was engineered to express an EGFR-targeting BiTE (cBiTE) antibody under the control of the major late promoter, leading to generation of ICOVIR-15K-cBiTE, which retained its oncolytic properties &lt;i&gt;in vitro&lt;/i&gt;. cBiTE expression and secretion was detected in supernatants from ICOVIR-15K-cBiTE–infected cells, and the secreted BiTEs bound specifically to both CD3&lt;sup&gt;+&lt;/sup&gt; and EGFR&lt;sup&gt;+&lt;/sup&gt; cells. In cell coculture assays, ICOVIR-15K-cBiTE–mediated oncolysis resulted in robust T-cell activation, proliferation, and bystander cell-mediated cytotoxicity. Notably, intratumoral injection of this cBiTE-expressing adenovirus increased the persistence and accumulation of tumor-infiltrating T cells &lt;i&gt;in vivo&lt;/i&gt;, compared with the parental virus lacking such effects. Moreover, in two distinct tumor xenograft models, combined delivery of ICOVIR-15K-cBiTE with peripheral blood mononuclear cells or T cells enhanced the antitumor efficacy achieved by the parental counterpart. Overall, our results show how arming oncolytic adenoviruses with BiTE can overcome key limitations in oncolytic virotherapy. &lt;i&gt;Cancer Res; 77(8); 2052–63. ©2017 AACR&lt;/i&gt;.&lt;/p&gt;&lt;/div&gt;
Variations of Organic Nitrogen Forms During Composting Process Using Three Stages Controlled by Temperature (2007)

Compost is one of the disposal ways for the municipal solid wastes(MSW),and the content of nitrogen(N) effects compost quality.Dynamic changes of organic nitrogen were discussed during MSW composting with three stages controlled by temperature(TSCT) technology.At different compost phases,the samples were taken for the determination of the nitrogen forms,and the effect of TSCT technology on nitrogen forms was studied.During MSW composting,the acid hydrolysis N,total N and amino acid N are a significant correlation and all decreased at the initial stage of composting.While at final stage composting,the content of acid hydrolysis N,amino acid N,aminosugar N and unidentified N of TSCT treatment increase by 10.67%,16.17%,7.17%,22.44% compared to CK.The results indicate the composting with TSCT technology would reduce the loss of N,improve the quality of MSW compost.
Immunotherapy: Engineering an Artificial T‐Cell Stimulating Matrix for Immunotherapy (Adv. Mater. 23/2019) (2019)

In article number 1807359, Hai-Quan Mao, Jonathan P. Schneck, and co-workers introduce an artificial T-cell stimulating matrix (aTM), a biomimetic hydrogel-based matrix that presents T-cell stimulatory signals. The aTM with optimized stimulatory properties effectively potentiates T-cell signaling, skews the phenotypes of both murine and human T cells, and ultimately generates anti-tumor T cells that suppress tumor growth in mice. The interaction of the T cells with the aTM and stimulatory ligands, leading to T-cell activation and expansion, is illustrated.
<title>Fabrication of organic light-emitting diodes using ITO anodes grown on polyethersulfone (PES) substrates by pulse-laser deposition</title> (2005)

Organic light-emitting diode (OLED) has a good candidate for next generation flat panel display (FPD). However, it is very difficult to fabricate high performance OLEDs on plastic substrate because its mechanical and thermal properties are very poor. Before the ITO deposition, we used a new plasma treatment for good contact with ITO and PES. PES substrate is stayed in vacuum above 12 hours to reduce humidity and unknown chemical gas. We successfully fabricate OLED on PES substrate using PLD-ITO anodes. We can observe more uniform and bright emission image from the OLED and fix the optimum conditions for fabrication process for OLED. Maximum electro luminescence (EL) and current density at a 100 cd/m<sup>2</sup> are 2500 cd/m<sup>2</sup>, 2mA/cm<sup>2</sup>, respectively and external quantum efficiency of OLED is about a 2.0%.
Microcracking in Ceramic/Ceramic Composites (1988)

A technique was developed to detect the onset, size, evolution, and anisotropy of surface microcracks in ceramic composite materials as a function of applied stress. The method is based on measuring the electrical resistance changes of a thin gold film sputtered on the surface of the composite as a function of stress. The technique was applied to the tensile surfaces of a 90/0/90, single crossply, 50 vol% SiC-LAS composite bars subjected to three-point bending. The resistance changes during the first loading cycle were found to be a strong function of the surface condition prior to testing. The onset of microcracking varied between 70 and 160 MPa. The anisotropy in the resistance changes in two orthogonal directions demonstrated that, for a given stress, the damage resulting from axial cracks was comparable to that of matrix cracks and occurred simultaneously. Furthermore, the matrix cracks were found to close more readily than the axial ones. The resistance changes were converted to crack lengths assuming that one large crack extended normal to the applied stress. The presence of such cracks was confirmed by SEM. How this method can be used nondestructively to assess the integrity of a finished surface is also discussed.
Divergence of laser-driven ion beams from nanometer thick foils (2013)

We report on experimental studies of divergence of proton beams from nanometer thick diamond-like carbon (DLC) foils irradiated by an intense laser with high contrast. Proton beams with extremely small divergence (half angle) of 2&deg; are observed in addition with a remarkably well-collimated feature over the whole energy range, showing one order of magnitude reduction of the divergence angle in comparison to the results from &mu;m thick targets. We demonstrate that this reduction arises from a steep longitudinal electron density gradient and an exponentially decaying transverse profile at the rear side of the ultrathin foils. Agreements are found both in an analytical model and in particle in cell simulations. Those novel features make nm foils an attractive alternative for high flux experiments relevant for fundamental research in nuclear and warm dense matter physics.
Protein kinase Cζ exhibits constitutive phosphorylation and phosphatidylinositol-3,4,5-triphosphate-independent regulation (2015)

Atypical protein kinase C (aPKC) isoenzymes are key modulators of insulin signalling, and their dysfunction correlates with insulin-resistant states in both mice and humans. Despite the engaged interest in the importance of aPKCs to type 2 diabetes, much less is known about the molecular mechanisms that govern their cellular functions than for the conventional and novel PKC isoenzymes and the functionally-related protein kinase B (Akt) family of kinases. Here we show that aPKC is constitutively phosphorylated and, using a genetically-encoded reporter for PKC activity, basally active in cells. Specifically, we show that phosphorylation at two key regulatory sites, the activation loop and turn motif, of the aPKC PKCζ in multiple cultured cell types is constitutive and independently regulated by separate kinases: ribosome-associated mammalian target of rapamycin complex 2 (mTORC2) mediates co-translational phosphorylation of the turn motif, followed by phosphorylation at the activation loop by phosphoinositide-dependent kinase-1 (PDK1). Live cell imaging reveals that global aPKC activity is constitutive and insulin unresponsive, in marked contrast to the insulin-dependent activation of Akt monitored by an Akt-specific reporter. Nor does forced recruitment to phosphoinositides by fusing the pleckstrin homology (PH) domain of Akt to the kinase domain of PKCζ alter either the phosphorylation or activity of PKCζ. Thus, insulin stimulation does not activate PKCζ through the canonical phosphatidylinositol-3,4,5-triphosphate-mediated pathway that activates Akt, contrasting with previous literature on PKCζ activation. These studies support a model wherein an alternative mechanism regulates PKCζ-mediated insulin signalling that does not utilize conventional activation via agonist-evoked phosphorylation at the activation loop. Rather, we propose that scaffolding near substrates drives the function of PKCζ.
Combining Expression and Comparative Evolutionary Analysis. TheCOBRAGene Family (2006)

Plant cell shape is achieved through a combination of oriented cell division and cell expansion and is defined by the cell wall. One of the genes identified to influence cell expansion in the Arabidopsis (Arabidopsis thaliana) root is the COBRA (COB) gene that belongs to a multigene family. Three members of the AtCOB gene family have been shown to play a role in specific types of cell expansion or cell wall biosynthesis. Functional orthologs of one of these genes have been identified in maize (Zea mays) and rice (Oryza sativa; Schindelman et al., 2001; Li et al., 2003; Brown et al., 2005; Persson et al., 2005; Ching et al., 2006; Jones et al., 2006). We present the maize counterpart of the COB gene family and the COB gene superfamily phylogeny. Most of the genes belong to a family with two main clades as previously identified by analysis of the Arabidopsis family alone. Within these clades, however, clear differences between monocot and eudicot family members exist, and these are analyzed in the context of Type I and Type II cell walls in eudicots and monocots. In addition to changes at the sequence level, gene regulation of this family in a eudicot, Arabidopsis, and a monocot, maize, is also characterized. Gene expression is analyzed in a multivariate approach, using data from a number of sources, including massively parallel signature sequencing libraries, transcriptional reporter fusions, and microarray data. This analysis has revealed that the expression of Arabidopsis and maize COB gene family members is highly developmentally and spatially regulated at the tissue and cell type-specific level, that gene superfamily members show overlapping and unique expression patterns, and that only a subset of gene superfamily members act in response to environmental stimuli. Regulation of expression of the Arabidopsis COB gene family members has highly diversified in comparison to that of the maize COB gene superfamily members. We also identify BRITTLE STALK 2-LIKE 3 as a putative ortholog of AtCOB.
Chemotherapeutic drug-specific alteration of microvascular blood flow in murine breast cancer as measured by diffuse correlation spectroscopy (2016)

The non-invasive, <i>in vivo</i> measurement of microvascular blood flow has the potential to enhance breast cancer therapy monitoring. Here, longitudinal blood flow of 4T1 murine breast cancer (N=125) under chemotherapy was quantified with diffuse correlation spectroscopy based on layer models. Six different treatment regimens involving doxorubicin, cyclophosphamide, and paclitaxel at clinically relevant doses were investigated. Treatments with cyclophosphamide increased blood flow as early as 3 days after administration, whereas paclitaxel induced a transient blood flow decrease at 1 day after administration. Early blood flow changes correlated strongly with the treatment outcome and distinguished treated from untreated mice individually for effective treatments.
Final words (2007)

After all that has been said, we get to this final chapter. By now, if you have read the book in detail, you know some of the strategies that can help make lectures successful and effective. However, there is much more to lecturing than what can be covered in any book. Lecturing requires persistence to keep on trying even after numerous failures. It requires practice and experience, just as practice and experience are required in order to become a competent artist. And finally, it requires keeping in mind why you are lecturing and why you are there. Just as a doctor is often faced with life and death questions regarding patients, so is a lecturer often faced with life altering questions regarding the audience. As a result, lecturers need to be active in what they do and how they lecture. They must avoid fear of trying new things and avoid getting stuck in lecturing local minima where their performance, while okay, could be substantially improved through further experiment and effort. In the next few sections, we will take a closer look at these and other general issues.
Effect of Cetyltrimethylammonium Bromide on the Oxidation of Phthalic Acid by in Aqueous Medium (2010)

The conventional ultraviolet-visible (UV)-visible spectrophotometric technique was used to monitor the kinetics of the oxidation of phthalic acid by permanganate in absence and presence of cationic micelles of cetyltrimethylammonium bromide (CTAB). Under pseudo-first order conditions ([phthalic acid] ≥ ), the oxidation kinetics follows a complicate reaction path (the reaction rate decreases monotonically with [phthalic acid] which suggest the formation of more than one permanganate- phthalic acid complexes, out of these one of the complex has resistance toward the oxidative cleavage). The micellar catalysis is attributed to the formation of ion-pair between CTAB sub-aggregates and reactants as well as increase in the concentration (solubilization) of both reactants and/or intermediate in the micellar pseudo phase. The changes observed in the direction of the rate constants-[H+] profile correspond to the acid catalyses. The catalytic effect of CTAB may be due to the solubilization and incorporation of both the reactants into the Stern layer of the micelles and results have discussed in terms of pseudo-phase model of the micelles proposed by Menger and Portnoy and modified by Bunton et al. Mechanisms consistent with the observed kinetic data have been proposed. The effects of electrolytes, MnCl2, NaF, NaBr, and NaNO3, have also been studied and discussed. On the bases of observed results, a suitable mechanism has been proposed.
Seamless reconstruction of part-based high-relief models from hand-drawn images (2018)

We present a new approach to reconstruction of high-relief surface models from hand-made drawings. Our method is tailored to an interactive modeling scenario where the input drawing can be separated into a set of semantically meaningful parts of which relative depth order is known beforehand. For this kind of input, our technique allows inflating individual components to have a semi-elliptical profile, positioning them to satisfy prescribed depth order, and providing their seamless interconnection. Compared to previous methods, our approach is the first that formulates this reconstruction process as a single non-linear optimization problem. Because its direct optimization is computationally challenging, we propose an approximate solution which delivers comparable results orders of magnitude faster enabling an interactive user workflow. We evaluate our approach on various hand-made drawings and demonstrate that it provides state-of-the-art quality in comparison with previous methods which require comparable user intervention.
Innenrücktitelbild: Selective and Wash‐Resistant Fluorescent Dihydrocodeinone Derivatives Allow Single‐Molecule Imaging of μ‐Opioid Receptor Dimerization (Angew. Chem. 15/2020) (2020)

Hochselektive fluoreszierende Liganden abgeleitet aus dem Schlafmohn, wurden von D. Calebiro, M. Decker et al. in ihrem Forschungsartikel auf S. 6014 entwickelt, um zu untersuchen, ob μ-Opioidrezeptoren (μ-ORs) Dimere auf der Plasmamembran bilden können. Die Verbindungen weisen eine lange Verweilzeit und hohe Affinität auf, und eine kleine Fraktion kurzlebiger Homodimere konnte mithilfe dieser chemischen Werkzeuge durch Einzelmolekülmikroskopie nachgewiesen werden.
A Simple Operation Approach for Modular Multilevel Converter Under Grid Voltage Swell in Medium-Voltage Microgrids (2019)

Modular multilevel converter (MMC) is drawing more and more attention in medium-voltage microgrid applications. Large-scale switching-off loads and grid faults may lead to the voltage swell in microgrid, where MMC should have the ride-through ability. In this paper, a simple operation approach with the fundamental and irregular zero-sequence voltage (ZSV) injection is proposed for MMC under grid voltage swell condition. First, the fundamental ZSV injection is applied to adjust the modulation margin of three phases where the amplitude values of modulation waves in each phase can be the same. Then, if the amplitudes with fundamental ZSV injection are still in over-modulation status, the new irregular ZSV injection is added to further enlarge the operation region. In addition, the limitation of the irregular ZSV injection is analyzed. Simulation and experimental results of MMC under different grid voltage swell conditions verify the effectiveness of the proposed operation approach.
Formation of Six-membered Aza-nickelacycles by Oxidative Addition of Cyclopropyl Imines to Nickel(0) (2011)

Abstract Cyclopropyl imines reacted with nickel(0) in the presence of IPr to give the expected six-membered aza-nickelacycles confirmed by X-ray crystallography. The molecular structure of aza-nickelacyles shows monomeric η1-aza-nickelenolate structures. The reaction of aza-nickelacycle with an enone occurred to generate an η3-oxa-allylnickel complex.
Phyllanthus urinaria ameliorates the severity of nutritional steatohepatitis both in vitro and in vivo (2007)

Hepatic oxidative stress plays a critical role in metabolic forms of steatohepatitis. Phyllanthus urinaria, an herbal medicine, has been reported to have potential antioxidant properties. We tested the effects of P. urinaria on nutritional steatohepatitis both in vitro and in vivo. Immortalized normal hepatocytes (AML-12) or primary hepatocytes were exposed to control, the methionine-and-choline-deficient (MCD) culture medium, in the presence or absence of P. urinaria for 24 hours. Hepatocyte triglyceride, release of alanine aminotransferase, lipoperoxides, and reactive oxygen species production were determined. Age-matched C57BL/6 and db/db mice were fed control or MCD diet for 10 days with or without P. urinaria. Hepatic steatosis, necroinflammation, triglycerides, and lipid peroxide levels were determined. Hepatic expression of inflammatory factors and lipid regulatory mediators were assayed. P. urinaria reduced steatosis and alanine aminotransferase (ALT) levels in culture of hepatocytes in a dose-dependent manner. Phyllanthus prevented MCD-induced hepatic fat accumulation and steatohepatitis in mice. This effect was associated with repressed levels of hepatic lipid peroxides, reduced expression of cytochrome P450-2E1, pro-inflammatory tumor necrosis factor alpha, interleukin-6, dampened activation of inflammatory c-Jun N-terminal kinase (JNK) and nuclear factor kappa B (NF-kappaB), increased expression of lipolytic cytochrome P450 (Cyp4a10), and suppressed transcriptional activity of lipogenic CCAAT/enhancer binding protein beta (C/EBPbeta). Hepatic acyl co-enzyme A oxidase that regulated hepatic beta-oxidation of fatty acid and other lipid regulators were not affected by P. urinaria. In conclusion, P. urinaria effectively alleviated the steatohepatitis induced by the MCD, probably through dampening oxidative stress, ameliorating inflammation, and decreasing lipid accumulation.
Optimal Resource Allocation for Data Service in CDMA Reverse Link (2007)

The optimal resource allocation policy is studied for non-real-time users in CDMA reverse link. The resource allocation policy of interest includes channel coding, spreading gain control and power allocation under the conventional receiver operation. The constraints in the optimization include peak transmit power of the mobile station, total received power at the base station and QoS in the form of minimum SINR for each user. The coding and spreading gain control can be separated from the power allocation strategy. Our results show that the optimal power allocation policy depends on the objective function: a greedy policy is optimal to maximize the sum of throughput from each user, whereas a fair policy is optimal to maximize the product of throughput from each user. A unified approach is taken to derive the optimal policies, and it can also be applied to other power allocation problems in CDMA reverse link. Numerical results of the channel capacity are presented for both objectives along with the effect of QoS constraints.
A digital video signal processor for color image sensors (2003)

The authors describe signal processor (DSP) for CCD (charge-coupled-device) cameras using a specified color filter array pattern. A block diagram of the DSP chip is shown. The chip has been designed and fabricated in a 2- mu m single-poly double-metal CMOS process. Eight scan-test registers were used at selected points in the processing chain to enable the entire chip to be tested, including ROMs and line delays, with 16 k vectors. The chip is functional at a maximum clock rate of 14.3 MHz. An image processed by the device is shown. The data path is designed with simple ripple-carry adders and dynamic registers. The on-chip programmable delay lines and 14.3-MHz clock-rate allow the chip to accommodate sensors for up to 768 active pixels, making it suitable for NTSC, CCIR 601 and PAL video standards.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Re: Generation of a Mouse Model of Von Hippel-Lindau Kidney Disease Leading to Renal Cancers by Expression of a Constitutively Active Mutant of HIF1α (2012)

No AccessJournal of UrologyInvestigative Urology1 Jun 2012Re: Generation of a Mouse Model of Von Hippel-Lindau Kidney Disease Leading to Renal Cancers by Expression of a Constitutively Active Mutant of HIF1α Anthony AtalaM.D. Anthony AtalaAnthony Atala More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.2503AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "Re: Generation of a Mouse Model of Von Hippel-Lindau Kidney Disease Leading to Renal Cancers by Expression of a Constitutively Active Mutant of HIF1α." The Journal of Urology, 187(6), pp. 2275–2276 © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 6June 2012Page: 2275-2276 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Anthony Atala More articles by this author Expand All Advertisement PDF downloadLoading ...
A voltage controlled oscillator using barium strontium titanate (BST) thin film varactor (2005)

Barium strontium titanate (BST) has a field-dependent permittivity and can be used as a dielectric in voltage tunable capacitors or varactors. These BST-based varactors are passive devices and have significantly different properties compared to semiconductor varactors. A voltage tunable oscillator using a BST thin film varactor was designed and characterized. The frequency of oscillation varied from 34.8 MHz to 44.5 MHz (28% tuning) upon application of 7 V tuning voltage. The VCO gain was 1.38 MHz/V and the 2nd harmonic was over 23 dB below the fundamental throughout the tuning range.