gebhart/patrepeval huggingface dataset:
Positive real controller design with H/sub ∞/ norm performance bound (2005)

This paper considers the positive real controller design for strictly positive real plants subject to a general H/sub /spl infin// norm performance bound. This problem is shown to be equivalent to a pair of small gain problems. A sufficient condition for their solutions is a standard H/sub /spl infin// small gain problem which can be readily solved. This procedure has been applied to the control of an experimental rotating flexible beam with good results.
Cysteine Protease Activity and Histamine Release from the Human Mast Cell Line HMC-1 Stimulated by Recombinant Streptococcal Pyrogenic Exotoxin B/Streptococcal Cysteine Protease (2002)

We constructed the expression vector pSK-SCP containing the streptococcal exotoxin B gene (spe b) which expressed protease activity. We showed that the recombinant streptococcal pyogenic exotoxin B/streptococcal cysteine protease (rSPE B/SCP) was secreted into the culture supernatant of the transformant and retained its SCP activity, which was equivalent to or greater than that of the naturally occurring molecule. The secreted rSPE B/SCP induced histamine release and degranulation of the human mast cell line HMC-1. This study may contribute to the understanding of the pathogenic role of SPE B/SCP in streptococcal infection and streptococcal toxic shock syndrome.
Oxidation and chemical state analysis of polycrystalline magnetron sputtered (Ti, Al)N films at ambient and liquid N2 temperatures (2000)

In order to improve the functional properties of hard coatings, recent investigations have been directed towards Ti–N based multicomponent materials. The nitride (Ti, Al)N, in particular, with a Ti:Al ratio of 1:1 seems to be a promising alternative to the widely used TiN, exhibits better oxidation resistance and hence improved performance over that of TiN. (Ti, Al)N coatings were dc sputter deposited onto 316SS substrates under ambient and liquid nitrogen temperatures. As deposited films were oxidized in a vertical fused-silica tube furnace in pure O2 flowing atmosphere at temperatures ranging from 700 to 900 °C. Scanning electron microscope and atomic force microscope images reveal information about the particle size and film thickness. X-ray photoelectron spectroscopy was employed to study the chemistry of the top few atomic layers in addition to compositional analysis and information on the details of chemical bonding. The difference in film stoichiometry are compared at two different deposition conditions thus reflecting their behavior under oxidizing conditions.
Using the Morphology of the Photoplethysmogram Envelope to Automatically Detect Hypovolemia (2006)

There currently is no clinically accepted noninvasive technique for detecting moderate blood loss. Clinicians instead normally rely on lagging indicators such as blood pressure and tachycardia. We propose to use changes in the morphology of the respiratory induced variation in the photoplethysmogram (PPG) to detect moderate hypovolemia in non-ventilated subjects. These changes were characterized by two statistically robust metrics that were developed to characterize the top and bottom envelope of the PPG. The first metric detects when the height of the top envelope becomes greater than the difference between the minimum of the top envelope and the maximum of the bottom envelope. The second metric robustly detects when the upper and lower envelopes synchronously rise or fall. The use of these metrics was then validated in nonintubated healthy volunteers with a lower-body negative pressure (LBNP) chamber which induces central hypovolemia by sequestering blood in the hips and lower extremities. Hypovolemia corresponding to sequestration of more than 1 liter of blood (LBNP > 60 mmHg) was consistently detected using these metrics before significant change in blood pressure, or tachycardia are observed
Integrating post‐transcriptional regulation into the embryonic stem cell gene regulatory network (2011)

Abstract Stem cell behavior is orchestrated as a multilayered, concert of gene regulatory mechanisms collectively referred to as the gene regulatory network (GRN). Via cooperative mechanisms, transcriptional, epigenetic, and post‐transcriptional regulators activate and repress gene expression to finely regulate stem cell self‐renewal and commitment. Due to their tractability, embryonic stem cells (ESCs) serve as the model stem cell to dissect the complexities of the GRN, and discern its relation to stem cell fate. By way of high‐throughput genomic analysis, targets of individual gene regulators have been established in ESCs. The compilation of these discrete networks has revealed convergent, multi‐dimensional gene regulatory mechanisms involving transcription factors, epigenetic modifiers, non‐coding RNA (ncRNA), and RNA‐binding proteins. Here we highlight the seminal genomic studies that have shaped our understanding of the ESC GRN and describe alternate post‐transcriptional gene regulatory mechanisms that require in depth analyses to draft networks that fully model ESC behavior. J. Cell. Physiol. 227: 439–449, 2012. © 2011 Wiley Periodicals, Inc.
Attacks on Encrypted Memory and Constructions for Memory Protection (2016)

The first part of the talk discusses some potential implications of attacks on the system memory of a computing platform. We show that encryption to protect privacy is not necessarily sufficient to protect against active attacks. In the second part of the talk, we explore the Memory Encryption Engine, which is part of Intel's Software Guard Extensions (SGX) technology.
High Temperature Structural Foam (1998)

The Aerospace Industry is experiencing growing demand for high performance polymer foam. The X-33 program needs structural foam insulation capable of retaining its strength over a wide range of environmental conditions. The High Speed Research Program has a need for low density core splice and potting materials. This paper reviews the state of the art in foam materials and describes experimental work to fabricate low density, high shear strength foam which can withstand temperatures from -220 degrees C to 220 degrees C. Commercially available polymer foams exhibit a wide range of physical properties. Some with densities as low as 0.066 g/cc are capable of co-curing at temperatures as high as 182 degrees C. Rohacel foams can be resin transfer molded at temperatures up to 180 degrees C. They have moduli of elasticity of 0.19 MPa, tensile strengths of 3.7 MPa and compressive strengths of 3.6 MPa. The Rohacel foams cannot withstand liquid hydrogen temperatures, however Imi-Tech markets Solimide\Registered foams which withstand temperatures from -250 degrees C to 200 degrees C, but they do not have the required structural integrity. The research activity at NASA Langley Research Center focuses on using chemical blowing agents to produce polyimide thermoplastic foams capable of meeting the above performance requirements. The combination of blowing agents that decompose at the minimum melt viscosity temperature together with plasticizers to lower the viscosity has been used to produce foams by both extrusion and oven heating. The foams produced exhibit good environmental stability while maintaining structural properties.
-Catenin is a NEK2 substrate involved in centrosome separation (2021)

β-Catenin plays important roles in cell adhesion and gene transcription, and has been shown recently to be essential for the establishment of a bipolar mitotic spindle. Here we show that β-catenin is a component of interphase centrosomes and that stabilization of β-catenin, mimicking mutations found in cancers, induces centrosome splitting. Centrosomes are held together by a dynamic linker regulated by Nek2 kinase and its substrates C-Nap1 (centrosomal Nek2-associated protein 1) and Rootletin. We show that β-catenin binds to and is phosphorylated by Nek2, and is in a complex with Rootletin. In interphase, β-catenin colocalizes with Rootletin between C-Nap1 puncta at the proximal end of centrioles, and this localization is dependent on C-Nap1 and Rootletin. In mitosis, when Nek2 activity increases, β-catenin localizes to centrosomes at spindle poles independent of Rootletin. Increased Nek2 activity disrupts the interaction of Rootletin with centrosomes and results in binding of β-catenin to Rootletin-independent sites on centrosomes, an event that is required for centrosome separation. These results identify β-catenin as a component of the intercentrosomal linker and define a new function for β-catenin as a key regulator of mitotic centrosome separation.
A low-power transmitter design for inductive data link with class-F switching operation (2007)

This letter presents a new design method of a low-power transmitter for inductive link. The proposed method introduces the concept of class-F RF amplifiers into an inductive data link. The power dissipation of the class-F transmitter is analyzed for carrier and symbol transition. It is revealed that the proposed transmitter consumes extremely low power of less than 0.2nJ/bit with BPSK modulation at 2.5M Sample/s and the communication distance of about 50cm.
Two-dimensional laboratory-scale DNS for knocking experiment using n-heptane at engine-like condition (2020)

A two-dimensional laboratory-scale DNS of a knocking experiment using a stoichiometric n-C7H16/O2/Ar mixture at engine-like condition with the latest reduced SIP isooctane kinetics was conducted. A compressible reactive flow solver PeleC combined with an in-house efficient chemical kinetics solver MACKS was used. The results of DNS were compared with a “knocking experiment by Kyushu University” conducted in a 14 × 14 × 80 mm rectangular constant volume chamber for the same mixture at an initial temperature of 480 K and pressure of 0.33 MPa. The comparisons showed the present DNS with the latest, validated reduced chemical kinetics successfully reproduced the knock onset timing within an error of 2% as well as the overall features of the experimental observations seen in the Schlieren images such as flame shape transitions, pressure history, and the characteristic faint increase in pressure due to the flame front attachment with the chamber wall. In addition, the present DNS captured that the cool flame ignition occurred homogeneously but accompanied by the rapid formation of density gradients vertical to the side walls in the unburned gas region.
PCB-Based Magnetometer as a Platform for Quantification of Lateral-Flow Assays (2019)

This work presents a proof-of-concept demonstration of a novel inductive transducer, the femtoMag, that can be integrated with a lateral-flow assay (LFA) to provide detection and quantification of molecular biomarkers. The femtoMag transducer is manufactured using a low-cost printed circuit board (PCB) technology and can be controlled by relatively inexpensive electronics. It allows rapid high-precision quantification of the number (or amount) of superparamagnetic nanoparticle reporters along the length of an LFA test strip. It has a detection limit of 10<sup>-10</sup> emu, which is equivalent to detecting 4 ng of superparamagnetic iron oxide (Fe<sub>3</sub>O<sub>4</sub>) nanoparticles. The femtoMag was used to quantify the hCG pregnancy hormone by quantifying the number of 200 nm magnetic reporters (superparamagnetic Fe<sub>3</sub>O<sub>4</sub> nanoparticles embedded into a polymer matrix) immuno-captured within the test line of the LFA strip. A sensitivity of 100 pg/mL has been demonstrated. Upon further design and control electronics improvements, the sensitivity is projected to be better than 10 pg/mL. Analysis suggests that an average of 10<sup>9</sup> hCG molecules are needed to specifically bind 10<sup>7</sup> nanoparticles in the test line. The ratio of the number of hCG molecules in the sample to the number of reporters in the test line increases monotonically from 20 to 500 as the hCG concentration increases from 0.1 ng/mL to 10 ng/mL. The low-cost easy-to-use femtoMag platform offers high-sensitivity/high-precision target analyte quantification and promises to bring state-of-the-art medical diagnostic tests to the point of care.
The MONitoring Dialysis Outcomes (MONDO) Initiative (2013)

While organizationally lean and low-cost, MONDO is the largest global dialysis database initiative to date, with a particular focus on high longitudinal data density and geographical diversity.
Supplementary Table 3 from SMAC Mimetic (JP1201) Sensitizes Non–Small Cell Lung Cancers to Multiple Chemotherapy Agents in an IAP-Dependent but TNF-α–Independent Manner (2023)

&lt;p&gt;PDF file - 57K, Combination indices (CI) were calculated using the Chou‐Talay method (Chou and Rideout, 1991). CI values that are less than 1 � 0.2 indicate synergy, values that are equal to 1 � 0.2 indicate additivity, and values that are greater than 1 � 0.2 indicate antagonism. Error was calculated by propagating the error from the drug sensitivity phenotypes from MTS data. Data represents average of �3 determinants.&lt;/p&gt;
Myocardial Cell Death in Human Diabetes (2000)

The renin-angiotensin system is upregulated with diabetes, and this may contribute to the development of a dilated myopathy. Angiotensin II (Ang II) locally may lead to oxidative damage, activating cardiac cell death. Moreover, diabetes and hypertension could synergistically impair myocardial structure and function. Therefore, apoptosis and necrosis were measured in ventricular myocardial biopsies obtained from diabetic and diabetic-hypertensive patients. Accumulation of a marker of oxidative stress, nitrotyrosine, and Ang II labeling were evaluated quantitatively. The diabetic heart showed cardiac hypertrophy, cavitary dilation, and depressed ventricular performance. These alterations were more severe with diabetes and hypertension. Diabetes was characterized by an 85-fold, 61-fold, and 26-fold increase in apoptosis of myocytes, endothelial cells, and fibroblasts, respectively. Apoptosis in cardiac cells did not increase additionally with diabetes and hypertension. Diabetes increased necrosis by 4-fold in myocytes, 9-fold in endothelial cells, and 6-fold in fibroblasts. However, diabetes and hypertension increased necrosis by 7-fold in myocytes and 18-fold in endothelial cells. Similarly, Ang II labeling in myocytes and endothelial cells increased more with diabetes and hypertension than with diabetes alone. Nitrotyrosine localization in cardiac cells followed a comparable pattern. In spite of the difference in the number of nitrotyrosine-positive cells with diabetes and with diabetes and hypertension, apoptosis and necrosis of myocytes, endothelial cells, and fibroblasts were detected only in cells containing this modified amino acid. In conclusion, local increases in Ang II with diabetes and with diabetes and hypertension may enhance oxidative damage, activating cardiac cell apoptosis and necrosis.
Perturbation Methods in Applied Mathematics. By J. KEVORKIAN and J. D. COLE. Springer, 1981. 558 pp. DM 96. (1984)

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Direct effects of a monoclonal B cell differentiation factor and of purified interleukin 1 on B cell differentiation. (1984)

We have previously described the presence of factors in mixed lymphocyte culture supernatants that induce activated but not resting human B cells to secrete Ig. In the present study, we describe the effects of a B cell differentiation factor in the supernatant (SN) of a T-T hybridoma (clone 7D5). We find that it induces Ig secretion by human B cells in the absence of T cells or monocytes. It acts only on activated B cells, because small (resting) B cells isolated by centrifugal counterflow elutriation do not respond to it, whereas the same cells do develop into Ig-secreting cells if activated in culture with Staphylococcus aureus Cowan I before exposure to SN 7D5. By using class-specific reagents in enzyme-linked immunosorbent assays, we found SN 7D5 to result in the secretion of significant amounts of IgM, IgG, and IgA. We also studied the effects of highly purified interleukin 1 on this differentiation process. Interleukin 1 by itself failed to induce Ig secretion by activated B cells, and its presence was not required for the induction of Ig secretion by SN 7D5. However, interleukin 1 consistently synergized with SN 7D5 in inducing Ig secretion by purified B cells.
Harmonic light scattering study reveals structured clusters upon the supramolecular aggregation of regioregular poly(3-alkylthiophene) (2019)

Abstract Solubilized poly(3-alkylthiophene)s are known to self-assemble into well-ordered supramolecular aggregates upon lowering the solvent quality. This supramolecular organization largely determines the optical and electronic properties of these polymers. However, despite numerous studies the exact mechanism and kinetics of the aggregation process and the role of external stimuli are still poorly understood. Classical characterization techniques such as electronic spectroscopy, dynamic light scattering, and diffraction-based techniques have not been able to provide a full understanding. Here we use second-harmonic scattering (SHS) and third-harmonic scattering (THS) techniques to investigate this supramolecular aggregation mechanism. Our results indicate that the actual supramolecular aggregation is preceded by the formation of structured polymer-solvent clusters consistent with a nonclassical crystallization pathway.
Casein Kinase 1D Encodes a Novel Drug Target in Hedgehog—GLI-Driven Cancers and Tumor-Initiating Cells Resistant to SMO Inhibition (2021)

(1) Background: Aberrant activation of the hedgehog (HH)-GLI pathway in stem-like tumor-initiating cells (TIC) is a frequent oncogenic driver signal in various human malignancies. Remarkable efficacy of anti-HH therapeutics led to the approval of HH inhibitors targeting the key pathway effector smoothened (SMO) in basal cell carcinoma and acute myeloid leukemia. However, frequent development of drug resistance and severe adverse effects of SMO inhibitors pose major challenges that require alternative treatment strategies targeting HH-GLI in TIC downstream of SMO. We therefore investigated members of the casein kinase 1 (CSNK1) family as novel drug targets in HH-GLI-driven malignancies. (2) Methods: We genetically and pharmacologically inhibited CSNK1D in HH-dependent cancer cells displaying either sensitivity or resistance to SMO inhibitors. To address the role of CSNK1D in oncogenic HH signaling and tumor growth and initiation, we quantitatively analyzed HH target gene expression, performed genetic and chemical perturbations of CSNK1D activity, and monitored the oncogenic transformation of TIC in vitro and in vivo using 3D clonogenic tumor spheroid assays and xenograft models. (3) Results: We show that CSNK1D plays a critical role in controlling oncogenic GLI activity downstream of SMO. We provide evidence that inhibition of CSNK1D interferes with oncogenic HH signaling in both SMO inhibitor-sensitive and -resistant tumor settings. Furthermore, genetic and pharmacologic perturbation of CSNK1D decreases the clonogenic growth of GLI-dependent TIC in vitro and in vivo. (4) Conclusions: Pharmacologic targeting of CSNK1D represents a novel therapeutic approach for the treatment of both SMO inhibitor-sensitive and -resistant tumors.
Distinct U-shape efficiency-versus-current curves in AlGaN-based deep-ultraviolet light-emitting diodes (2015)

The efficiency of an AlGaN deep-ultraviolet light-emitting diode with peak emission wavelength of 285 nm is investigated as a function of current over a wide range of temperatures (110 K to 300 K). We find that the efficiency-versus-current curve exhibits unique and distinct features over the entire temperature range including three points of inflection. At low temperatures, the change in slope in the efficiency-versus-current curve is particularly pronounced producing a minimum in the efficiency after which the efficiency rises again. Furthermore, at high current density, the low-temperature efficiency exceeds the room-temperature efficiency. The feature-rich efficiency-versus-current curve is consistent with an enhancement in p-type conductivity by field-ionization of acceptors that occurs in the high-injection regime and is particularly pronounced at low temperatures. Differential conductivity measurements show a marked rise in the high-injection regime that is well correlated to the minimum point in the efficiency-versus-current curve.
Glucagon-like peptide-1(7-36)-amide confers glucose sensitivity to previously glucose-incompetent beta-cells in diabetic rats: in vivo and in vitro studies (1997)

The effects of glucagon-like peptide-1(7-36)-amide (GLP-1) on cAMP content and insulin release were studied in islets isolated from diabetic rats (n0-STZ model) which exhibited impaired glucose-induced insulin release. We first examined the possibility of re-activating the insulin response to glucose in the beta-cells of the diabetic rats using GLP-1 in vitro. In static incubation experiments, GLP-1 amplified cAMP accumulation (by 170%) and glucose-induced insulin release (by 140%) in the diabetic islets to the same extent as in control islets. Using a perifusion procedure, GLP-1 amplified the insulin response to 16.7 mM glucose by diabetic islets and generated a clear biphasic pattern of insulin release. The incremental insulin response to glucose in the presence of GLP-1, although lower than corresponding control values (1.56 +/- 0.37 and 4.53 +/- 0.60 pg/min per ng islet DNA in diabetic and control islets respectively), became similar to that of control islets exposed to 16.7 mM glucose alone (1.09 +/- 0.15 pg/min per ng islet DNA). Since in vitro GLP-1 was found to exert positive effects on the glucose competence of the residual beta-cells in the n0-STZ model. we investigated the therapeutic effect of in vivo GLP-1 administration on glucose tolerance and glucose-induced insulin release by n0-STZ rats. An infusion of GLP-1 (10 ng/min per kg; i.v.) in n0-STZ rats enhanced significantly (P < 0.01) basal plasma insulin levels, and, when combined with an i.v. glucose tolerance and insulin secretion test, it was found to improve (P < 0.05) glucose tolerance and the insulinogenic index, as compared with the respective values of these parameters before GLP-1 treatment.
Reduced urinary epidermal growth factor levels in Snell dwarf mutant mice (1986)

Because of findings implicating thyroid and growth hormones in the regulation of urinary EGF concentration, we determined urine and kidney EGF concentrations and relative kidney weights in both sexes of 8 weeks old dwarf mutant mice of the Snell strain and their normal littermates. Both male and female dwarfs had subnormal urine and kidney EGF concentrations. A female greater than male sex difference in both concentrations was present in the normal mice, but not in the dwarfs. The male greater than female sex difference in relative kidney weight, which has been demonstrated in normal mice of the Swiss-Webster strain, was not present in the normal or dwarf Snell mice.
Supplementary Legend from Patients with Biallelic BRCA1/2 Inactivation Respond to Olaparib Treatment Across Histologic Tumor Types (2023)

&lt;p&gt;Supplementary Legend&lt;/p&gt;
Online Feature Extraction for Evolving Intelligent Systems (2010)

This chapter contains sections titled: Introduction Incremental Principal Component Analysis (IPCA) Chunk Incremental Principal Component Analysis (CIPCA) Performance Evaluation Conclusion and Future Work References
Synthesis of Carbazoles by Tandem C-H Activation and Buchwald-Hartwig Amination (2006)

A highly efficient synthetic route to substituted carbazoles is reported. Starting from readily available 2-acetamidobyphenyls, the reaction proceeds via a tandem palladium-catalyzed C-H activation, Buchwald-Hartwig amination and Wacker reoxidation process under mild conditions to give a variety of substituted carbazoles in 41-98% yields with excellent regioselectivities. A simple plausible mechanism involving intermediates A and B has been suggested.