gebhart/patrepeval huggingface dataset:
Time-course global proteome analyses reveal an inverse correlation between Aβ burden and immunoglobulin M levels in the APPNL-F mouse model of Alzheimer disease (2017)

Alzheimer disease (AD) stands out amongst highly prevalent diseases because there is no effective treatment nor can the disease be reliably diagnosed at an early stage. A hallmark of AD is the accumulation of aggregation-prone amyloid β peptides (Aβ), the main constituent of amyloid plaques. To identify Aβ-dependent changes to the global proteome we used the recently introduced APPNL-F mouse model of AD, which faithfully recapitulates the Aβ pathology of the disease, and a workflow that interrogated the brain proteome of these mice by quantitative mass spectrometry at three different ages. The elevated Aβ burden in these mice was observed to cause almost no changes to steady-state protein levels of the most abundant >2,500 brain proteins, including 12 proteins encoded by well-confirmed AD risk loci. The notable exception was a striking reduction in immunoglobulin heavy mu chain (IGHM) protein levels in homozygote APPNL-F/NL-F mice, relative to APPNL-F/wt littermates. Follow-up experiments revealed that IGHM levels generally increase with age in this model. Although discovered with brain samples, the relative IGHM depletion in APPNL-F/NL-F mice was validated to manifest systemically in the blood, and did not extend to other blood proteins, including immunoglobulin G. Results presented are consistent with a cause-effect relationship between the excessive accumulation of Aβ and the selective depletion of IGHM levels, which may be of relevance for understanding the etiology of the disease and ongoing efforts to devise blood-based AD diagnostics.
Serum levels of major basic protein in patients with or without eosinophilia: measurement by enzyme‐linked immunosorbent assay (1994)

A bone marrow proteoglycan (BMPG) has been purified which consists of the same amino acid sequence as that of pro-MBP, and produced two anti-BMPG mAbs. Serum levels of major basic protein (MBP), a cationic protein rich in the eosinophil granule, were measured in patients with eosinophilia or allergic diseases by an enzyme-linked immunosorbent assay (ELISA) using these mAbs. The serum levels of MBP in patients with eosinophilia (n = 64) and in those with allergic diseases without eosinophilia (n = 32) were elevated significantly (P < 0.001 and P = 0.038, respectively). There was a weak positive correlation between the serum levels of MBP and the eosinophil counts in the patients with eosinophilia (r = 0.38). Among these patients, extremely high serum levels of MBP were found in those with hypereosinophilic syndrome (HES) and Kimura's disease. Serum levels of MBP decreased more slowly than the eosinophil counts in patients with eosinophilia when treated by glucocorticoids. We conclude that measurement of serum levels of MBP is useful in evaluating the total-body proliferation and infiltration of eosinophils more accurately than following-up the eosinophil counts alone.
Oral alloantigen exposure promotes donor-specific tolerance in a mouse model of minor mismatched skin transplantation (2022)

Abstract Oral tolerance is a powerful, non-invasive route to induce immune tolerance, yet it has been unsuccessful to promote graft acceptance in rodent models of transplantation. To this end, we developed a mouse model where the model antigen ovalbumin (OVA) was introduced orally, in a regimen designed to improve bioavailability, duodenal lymph node drainage and prolong duration of antigen exposure, prior to transplantation with skin grafts constitutively expressing membrane-bound OVA. We found that oral OVA treatment pre-transplantation promoted permanent graft acceptance as well as linked tolerance to skin grafts expressing OVA coupled to the additional model antigen 2W. Tolerance was donor-specific, as secondary donor-matched, but not third-party grafts were spontaneously accepted in the absence of additional immunosuppression. Treatment with this oral OVA regimen promoted an anergic phenotype in OVA-reactive CD8+ T cells, generation of iTregs, and dysfunction of OVA-reactive CD4+ T cells pre-transplantation. The tolerance induced by oral OVA was robust, as anti-CD25/anti-PD-L1 at the time of transplantation failed to prevent skin graft acceptance. Mechanistically, we revealed a critical role for the proximal gut draining lymph nodes (gLNs) in mediating this effect, as an intestinal infection that drains to the proximal gLNs prevented the induction of transplantation tolerance. Our study extends previous work applying oral antigen exposure to transplantation and serves as proof of concept that the systemic immune mechanisms supporting oral tolerance are sufficient to promote long-term graft acceptance. Regimens that improve bioavailability and duration of oral alloantigen administration may hold promise in the clinic. This work was supported by a NIAID grant to ASC and MLA (P01AI-97113). Peter Wang was supported by undergraduate research grants from the Katen Scholars Program and the Dean’s Fund for Undergraduate Research.
A Cardiac Phantom and Pulsatile Flow Pump for Magnetic Resonance Imaging Studies (1988)

Fast scan magnetic resonance imaging (MRI) acquisitions are a rapid noninvasive means of evaluating the cardiovascular system. Because the appearance of flowing blood is highly variable, the interpretation of these images is sometimes difficult. A nonferromagnetic phantom that could generate lifelike pulsatile flow and also simulate the motions of the beating heart would facilitate image interpretation. This paper describes an MRI-compatible cardiovascular phantom that mimics the motions of the heart and also creates physiologic pulsatile flow. The phantom consists of a ventricle and an air pump that drives it. The pump is connected to the ventricle with seven meters of air hose so that the pump (which has ferromagnetic parts) can be placed outside the magnet room. The ventricle is placed in an airtight Plexiglas cylinder and the pump alternately pressurizes and depressurizes the cylinder, driving fluid in and out of the ventricle. The motions of the ventricular wall simulate the motions of the heart, and the pulsatile flow generated is of physiologic velocities and volumes. This phantom also can be used with other methods of evaluating cardiovascular function, such as MUGAS, angiography, and Doppler, allowing correlation between MRI and other modalities. Finally, the phantom can be used to study almost any aspect of cardiovascular function from pulsatile flow velocity to ventricular studies (ejection fractions, cardiac output, wall motion) and even studies of stenotic or regurgitant valves.
Molecular yardsticks. Synthesis of extended equilibrium transfer alkylating cross-link reagents and their use in the formation of macrocycles (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMolecular yardsticks. Synthesis of extended equilibrium transfer alkylating cross-link reagents and their use in the formation of macrocyclesStephen J. Brocchini, Martin. Eberle, and Richard G. LawtonCite this: J. Am. Chem. Soc. 1988, 110, 15, 5211–5212Publication Date (Print):July 1, 1988Publication History Published online1 May 2002Published inissue 1 July 1988https://pubs.acs.org/doi/10.1021/ja00223a061https://doi.org/10.1021/ja00223a061research-articleACS PublicationsRequest reuse permissionsArticle Views617Altmetric-Citations50LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
The <i>hva1Δ</i> strain has a hypervirulent phenotype. (2016)

&lt;p&gt;Mouse fungal burden in the spleen (A), brain (B) and lungs (C) of mice infected intravenously with the pre-passage H99W, the &lt;i&gt;hva1Δ&lt;/i&gt; and &lt;i&gt;hva1Δ+HVA1&lt;/i&gt; strains. Six mice were infected for each group. Error bars depict standard deviation. D) Survival data for mice infected intravenously with the pre-passage H99W, the &lt;i&gt;hva1Δ&lt;/i&gt; and &lt;i&gt;hva1Δ+HVA1&lt;/i&gt; strains. Ten mice were infected for each group (only 9 mice reported for H99W).&lt;/p&gt;
Expression of mucosal addressin cell adhesion molecule-1 (MAdCAM-1) in acute and chronic inflammation (1999)

The objective of this study was to quantify, in vivo, constitutive and tumor necrosis factor alpha (TNF-alpha)-enhanced expression of mucosal addressin cell adhesion molecule-1 (MAdCAM-1) in different tissues from healthy wild-type mice (C57BL/6) as well as interleukin-10 (IL-10)-deficient mice with and without active colitis. Using the dual radiolabel monoclonal antibody technique, we found substantial constitutive expression of MAdCAM-1 in the intestine, colon, and mesenteric lymph nodes. MAdCAM-1 expression in these tissues was significantly enhanced, in a time-dependent manner, by systemic administration of TNF-alpha. Maximum surface expression was observed at 18 h after TNF-alpha administration and remained significantly elevated at 48 h post-TNF-alpha injection. No significant constitutive nor TNF-alpha-induced expression of MAdCAM-1 was detected in skeletal muscle, brain, or heart. In IL-10-deficient (IL-10 k/o) mice with no clinical or histological evidence of colitis, constitutive and TNF-alpha-induced expression of MAdCAM-1 in the intestine, cecum, and colon was not different from those values obtained with healthy wild-type controls. IL-10-deficient mice with active colitis exhibited a four- to fivefold greater expression of MAdCAM-1 in the cecum and colon compared with their healthy controls or to IL-10 k/o mice with no evidence of colitis. Taken together, these data demonstrate that TNF-alpha enhances surface expression of MAdCAM-1 in intestinal and colonic tissues to the same extent in both wild-type and IL-10 k/o mice with no colonic inflammation, whereas IL-10 k/o mice with active colitis exhibited a profound up-regulation of MAdCAM-1 in the colon.
Modeling entity evolution for temporal record matching (2014)

Temporal record matching recognizes that if the entities represented by the records change over time, approaches that use temporal information may do better than approaches that do not. Any such temporal matching method relies at its heart on a temporal model that captures information about how entities evolve. In their pioneering work, Li {\it et al.} used an efficiently computable model that simply tries to predict if an attribute is expected to change over a given time interval. In our work, we propose and evaluate a more detailed model that focuses on the probability that a given attribute value reappears over time. The intuition here is that an entity might change its attribute value in the way that is dependent on its past values. In addition, our model considers sets of records (rather than simply pairs of records) to improve robustness and accuracy. Experimental results show that the resulting approach improves both accuracy and resistance to noise while incurring a minimal overhead.
Graded-index polymer-based waveguide lens working at visible wavelengths on GaAs substrate for optoelectronic interconnects (1993)

A polymer-based graded index (GRIN with n=1.4–1.6) waveguide lens was demonstrated on a semi-insulating GaAs substrate. The transparent bandwidth of the polymer film (photolime gel) is from ∼280 to ∼2800 nm. Therefore, UV (ultraviolet), visible and near-IR (infrared) light can be employed as the signal carrier wavelength. In this letter, an off-axis chirped grating lens working at 632.8 nm with 35 mm focal length, 680/mm chirp rate, and angular field of view of 6° was demonstrated. Measured focal spot size of the main lobe is 5.6 μm which is very close to the theoretical prediction (4.2 μm). The crosslink-induced index modulation is as high as 0.2. Therefore, an array of chirped phase grating lenses can be multiplexed onto the same holographic emulsion for one- and two-dimensional massive fanout optical interconnects and signal processing.
Determinant of antithrombin consumption in cardiac operations requiring cardiopulmonary bypass (2004)

Ranucci, M.; Isgrò, G.; Cazzaniga, A.; Soro, G.; De Benedetti, D.; Pazzaglia, A. Author Information
Swelling and static-dynamic mechanical behavior of mica-reinforced linear and star-branched polybutadiene composites (1997)

Physical properties of mica-reinforced linear (LPB) and star-branched polybutadiene (SPB) composites have been studied with special reference to the effect of silane coupling agent, degree of crosslinking, and degree of mica loading, as well as the molecular architecture of polybutadienes. Tensile properties increase and swelling decreases with addition of mica for linear polybutadiene. Star-branched polybutadiene shows a reverse behavior, especially beyond 5% of mica. The improvements in mechanical properties are more pronounced in the case of silane-treated mica compositions of both type of polybutadienes. Dynamic mechanical spectra were obtained for linear and star-branched polybutadienes. Effects of mica loading and silane treatment on dynamic moduli are discussed. Dynamic mechanical moduli (E′, E′) of composites increase with increasing mica content for linear polybutadiene but decrease for star-branched polybutadiene beyond certain mica loadings. Effective damping regions were determined in terms of frequency and temperature. The glass transition temperature (Tg) increased slightly, and the damping peak (tan δ) broadened due to the rubber—filler interaction, especially after silane treatment for both polymers. © 1997 John Wiley & Sons, Inc. J Appl Polym Sci 66: 1943–1952, 1997
Eye-Tracked Extraocular Camera for Retinal Prostheses (2015)

The optical design and system integration of an eye-tracked wide-angle extraocular camera for use in conjunction with intraocular retinal prostheses is described. This integrated system is capable of restoring foveation for those with retinal prostheses.
HQ Solid State Li-Gas (Air,SO2) Batteries for Transportation and Energy Storage (2010)

Abstract not Available.
An intranasally administrated SARS-CoV-2 beta variant subunit booster vaccine prevents beta variant viral replication in rhesus macaques (2021)

ABSTRACT Emerging of SARS-CoV-2 variants and waning of vaccine/infection-induced immunity poses threats to curbing the COVID-19 pandemic. An effective, safe, and convenient booster vaccine will be needed. We hypothesized that a variant-modified mucosal booster vaccine might induce local immunity to prevent SARS-CoV-2 infection at the port of entry. The beta-variant is hardest to cross-neutralize. Herein we assessed the protective efficacy of an intranasal booster composed of beta variant-spike protein S1 with IL-15 and TLR agonists in previously immunized macaques. The macaques were first vaccinated with Wuhan strain S1 with the same adjuvant. One year later, negligibly detectable SARS-CoV-2-specific antibody remained. Nevertheless, the booster induced vigorous humoral immunity including serum- and bronchoalveolar lavage (BAL)-IgG, secretory nasal- and BAL-IgA, and neutralizing antibody against the original strain and/or beta variant. Beta-variant S1-specifc CD4 + and CD8 + T cell responses were also elicited in PBMC and BAL. Following SARS-CoV-2 beta variant challenge, the vaccinated group demonstrated significant protection against viral replication in the upper and lower respiratory tracts, with almost full protection in the nasal cavity. The fact that one intranasal beta-variant booster administrated one year after the first vaccination provoked protective immunity against beta variant infections may inform future SARS-CoV-2 booster design and administration timing.
Sex Differences in the Blood Transcriptome Identify Robust Changes in Immune Cell Proportions with Aging and Influenza Infection (2019)

Sex differences in autoimmunity and infection suggest that a better understanding of molecular sex differences will improve the diagnosis and treatment of immune-related disease. We identified 144 differentially expressed genes, referred to as immune sex expression signature (iSEXS), between human males and females using an integrated multi-cohort analysis of blood transcriptome profiles from six discovery cohorts from five continents with 458 healthy individuals. We validated iSEXS in 11 additional cohorts of 524 peripheral blood samples. When we separated iSEXS into genes located on sex chromosomes (XY-iSEXS) or autosomes (autosomal-iSEXS), both modules distinguished males and females. iSEXS reflects sex differences in immune cell proportions, with female-associated genes showing higher expression by CD4<sup>+</sup> T cells and male-associated genes showing higher expression by myeloid cells. Autosomal-iSEXS detected an increase in monocytes with age in females, reflected sex-differential immune cell dynamics during influenza infection, and predicted antibody response in males, but not females.
Darstellung von malignen Tumoren im Abdomen unter Zuhilfenahme radioaktiv markierter monoklonaler Antikörper (1984)

The production of monoclonal antibodies by the hybrid technique introduced by Köhler and Milstein in 1975, led to new dimensions in the diagnosis and therapy of malignant diseases. Labelling such monoclonal antibodies, directed against tumour cells, by a radioactive isotope, makes them usable for visualising malignant tumours and their metastases. Early detection of cancer may represent a major step forward in cases where early detection is not possible with common noninvasive methods, such as ovarian cancer in gynaecological oncology. Radioimmunoscintigrams (RIS) were performed in seven patients, all of whom were suspect of having ovarian cancer. The RIS-results showed very good correlations with the operation reports. According to these first results we assume that the RIS method using I123-labelled monoclonal antibodies directed against tumour cells may be successful in the early detection of ovarian cancer. Other important information supplied by radioimmunoscanning is determination of tumour status, localisation, extent or metastatic spread.
Brain stiffens post mortem (2018)

Alterations in brain rheology are increasingly recognized as a diagnostic marker for various neurological conditions. Magnetic resonance elastography now allows us to assess brain rheology repeatably, reproducibly, and non-invasively in vivo. Recent elastography studies suggest that brain stiffness decreases one percent per year during normal aging, and is significantly reduced in Alzheimer's disease and multiple sclerosis. While existing studies successfully compare brain stiffnesses across different populations, they fail to provide insight into changes within the same brain. Here we characterize rheological alterations in one and the same brain under extreme metabolic changes: alive and dead. Strikingly, the storage and loss moduli of the cerebrum increased by 26% and 60% within only three minutes post mortem and continued to increase by 40% and 103% within 45 minutes. Immediate post mortem stiffening displayed pronounced regional variations; it was largest in the corpus callosum and smallest in the brainstem. We postulate that post mortem stiffening is a manifestation of alterations in polarization, oxidation, perfusion, and metabolism immediately after death. Our results suggest that the stiffness of our brain-unlike any other organ-is a dynamic property that is highly sensitive to the metabolic environment. Our findings emphasize the importance of characterizing brain tissue in vivo and question the relevance of ex vivo brain tissue testing as a whole. Knowing the true stiffness of the living brain has important consequences in diagnosing neurological conditions, planning neurosurgical procedures, and modeling the brain's response to high impact loading.
Exploring unbalanced electrode configurations for electrochromic devices (2017)

A new unbalanced electrochromic configuration is demonstrated, allowing minimum energy consumption while maintaining optimum optoelectrochemical performance.
Venous overload choroidopathy: A hypothetical framework for central serous chorioretinopathy and allied disorders (2021)

In central serous chorioretinopathy (CSC), the macula is detached because of fluid leakage at the level of the retinal pigment epithelium. The fluid appears to originate from choroidal vascular hyperpermeability, but the etiology for the fluid is controversial. The choroidal vascular findings as elucidated by recent optical coherence tomography (OCT) and wide-field indocyanine green (ICG) angiographic evaluation show eyes with CSC have many of the same venous patterns that are found in eyes following occlusion of the vortex veins or carotid cavernous sinus fistulas (CCSF). The eyes show delayed choroidal filling, dilated veins, intervortex venous anastomoses, and choroidal vascular hyperpermeability. While patients with occlusion of the vortex veins or CCSF have extraocular abnormalities accounting for the venous outflow problems, eyes with CSC appear to have venous outflow abnormalities as an intrinsic phenomenon. Control of venous outflow from the eye involves a Starling resistor effect, which appears to be abnormal in CSC. Similar choroidal vascular abnormalities have been found in peripapillary pachychoroid syndrome. However, peripapillary pachychoroid syndrome has intervortex venous anastomoses located in the peripapillary region while in CSC these are seen to be located in the macular region. Spaceflight associated neuro-ocular syndrome appears to share many of the pathophysiologic problems of abnormal venous outflow from the choroid along with a host of associated abnormalities. These diseases vary according to their underlying etiologies but are linked by the venous decompensation in the choroid that leads to significant vision loss. Choroidal venous overload provides a unifying concept and theory for an improved understanding of the pathophysiology and classification of a group of diseases to a greater extent than previous proposals.