gebhart/patrepeval huggingface dataset:
The Choline Binding Site of Phospholipase C (Bacillus cereus):  Insights into Substrate Specificity (2000)

The phosphatidylcholine-preferring phospholipase C from Bacillus cereus (PLC(Bc)) is a 28.5 kDa enzyme with three zinc ions in its active site. The roles that a number of amino acid residues play as zinc ligands and in binding and catalysis have been elucidated. Recent mechanistic studies indicate that the rate of the reaction is limited by a proton-transfer step during chemical hydrolysis and not substrate binding or product release. An X-ray structure of PLC(Bc) complexed with a phosphonate inhibitor related to phosphatidylcholine revealed that the three amino acid residues Glu4, Tyr56, and Phe66 comprise the choline binding pocket. However, because the contributions that these three residues make to substrate recognition and specificity were unknown, a series of site-specific mutants for Glu4, Tyr56, and Phe66 were constructed by PCR mutagenesis. On the basis of a comparison of their respective CD spectra and melting temperatures, it appears that the mutants adopt folded structures in solution that are virtually identical to that of wild-type PLC(Bc). The kinetic parameters k(cat) and K(m) for the hydrolysis of the three soluble substrates 1, 2-dihexanoyl-sn-glycero-3-phosphocholine (C6PC), 1, 2-dihexanoyl-sn-glycero-3-phosphoethanolamine (C6PE), and 1, 2-dihexanoyl-sn-glycero-3-phospho-L-serine (C6PS) at concentrations below their corresponding critical micelle concentration (cmc) values were determined for each mutant. Replacement of Phe66 with a nonaromatic residue dramatically decreased k(cat) (approximately 200-fold) and reduced PLC(Bc) activity toward C6PC, C6PE, and C6PS, whereas changes to Glu4 and Tyr56 typically led to much more modest losses in catalytic efficiencies. Mutations of Glu4 had relatively little effect upon k(cat) and K(m) for C6PS, but they significantly influenced K(m) for C6PC and C6PE. Replacing Tyr56 with nonaromatic residues also affects catalytic efficiency, albeit to a much lesser degree than the corresponding changes at position 66. However, the presence of an aromatic residue at position 56 seems to confer some substrate selectivity for C6PC and C6PE, which bear a positive charge on the headgroup, relative to C6PS, which has no net charge on the headgroup; this increase in specificity arises largely from a reduced k(cat) for C6PS.
Does Frequent Low Resolution Feedback Outperform Infrequent High Resolution Feedback for Multiple Antenna Beamforming Systems? (2011)

Multiple antenna systems that adapt to the channel conditions are known to provide numerous rate and reliability benefits. In frequency division duplexing, the transmitter typically learns about the channel conditions using a small amount of feedback (called limited feedback) sent on the reverse link. While feedback is well studied, there has been only limited work addressing how feedback techniques should be modified depending on the amount of mobility. This paper concerns a signaling scheme for limited feedback multiple antenna wireless communications taking the temporal correlation into account during feedback design. We refer to this as using an adaptive feedback period (AFP) scheme. In the AFP scheme, the transmitter and receiver reuse the past channel state information (CSI) as side information. Feedback to be used for several channel uses is sent from the receiver to the transmitter using a predetermined feedback update period. The feedback update period is determined by characterizing the temporal correlation statistic, so that the proposed AFP scheme outperforms the traditional limited feedback approach, which we refer to as the minimal feedback period (MFP) scheme. To measure the performance, the average effective SNR is considered. Bounds on the feedback update period, correlation coefficient, and feedback rate needed for the AFP scheme to outperform the MFP scheme are derived. Moreover, criteria to initiate the control of the feedback update period are obtained. Results on the average effective SNR are used to develop bounds on the feedback update period using the capacity loss. It is also shown that in the large system limit the feedback update periods required for both the average effective SNR and capacity loss converge to the same bound. These results verify that infrequent high resolution feedback is sometimes preferable to frequent low resolution feedback.
Effect of Tb3+ co-doping and particle size on K2Ca2(SO4)3:EU phosphor (2003)

Abstract K2Ca2(SO4)3 doped with Eu, and co-doped with Tb were prepared by the solid state diffusion method. The nanoparticles of these phosphors were also prepared by the chemical co-precipitation method. The formation of the compounds was confirmed by XRD. The particle size was calculated by the broadening of the XRD peaks using Scherrer's formula. The particle size was found to be around 20 nm. Thermoluminescence (TL) was studied to see the effect of co-doping and particle size. Tb3+ co-doping decreases the intensity in the Eu2+ doped phosphor due to the energy transfer and multiple de-excitations through various radiative and non-radiative processes. The K2Ca2(SO4)3:Eu,Tb phosphor is found to be 0.33 times more sensitive than TLD-700H, but around 15 times more than LiF-TLD 100, and 7 times more than CaSO4:Dy. The effective atomic number Zeff is around 15, which is again comparable to CaSO4:Dy. However, very low sensitivity was observed in the case of nanoparticles. The decrease in the sensitivity is attributed to the particle size effect i.e., the volume-to-surface ratio. Study of photoluminescence (PL) of the material is also carried out. Keywords: TLD phosphorK2Ca2(SO4)3:Eu,Tb, nanoparticlesCo-dopingEnergy transfer Acknowledgement One of the authors (Numan) is very grateful to the Indian Council for the Cultural Relation (ICCR), India for financial assistance. We are also thankful to Dr. Amit Roy, the director of the Nuclear Science Centre (NSC), Delhi for permitting us to use the facilities at the centre.
Peripartum cardiomyopathy (2018)

Abstract Pregnancy-related heart disease is increasing worldwide and peripartum cardiomyopathy (PPCM) is an important contributor to early (<42 days postpartum) and late (up to 1 year postpartum) maternal death. PPCM is a potentially life-threatening condition presenting with heart failure secondary to left ventricular dysfunction towards the end of pregnancy, or in the months following delivery, where no other cause of heart failure is identified. It is a diagnosis of exclusion. Incidence and prognosis varies according to geography and is likely due to multiple factors including also inherited or acquired cardiomyopathy-associated mutations. It seems that PPCM also shares common pathological pathways. For example, a 'multiple-hit' model including systemic angiogenic imbalance that derives from the oxidative stress-cathepsin D-16 kDa prolactin cascade and additional antiangiogenic factors plays a key role in the development of PPCM in experimental models and in humans suggests that a therapeutic approach involving blockade of this pathway with bromocriptine may be a novel disease-specific approach. Despite ongoing research, numerous uncertainties regarding the incidence, pathophysiology, treatment, and prognosis of PPCM patients remain, indicating the need for further investigation. The ongoing global registry on PPCM, under the umbrella of the EuroObservational research programme, has provided novel information.
Bistable electrowetting displays (2011)

The electrowetting D³ technology ("Droplet-Driven-Displays") was invented by ADT. Those displays have features like bistability and highest reflectivity, which offer unique application options for real "No Power ("green") applications ranging from indicators to billboards. This paper describes the fundamentals of D³ EW displays (Passive Matrix, no Active Matrix needed) and system design incl. electronic driving using commercial ICs. We recently demonstrated all backlight modes (reflective, transflective and transmissive). Pixel size ranges from 0.5 to 10 mm and a wide temperature range is covered; color is realized by subtractive CMY stacks with printer-like gamut. The measurements of ambient light contrast ratio and color gamut clearly demonstrates the excellent performance of ADT's electrowetting displays for applications like "No Power" bistable LED indicator replacements and sunlight readable electronic billboards.
Localization of renal vitamin K-dependent gamma-glutamyl carboxylase to tubule cells. (1982)

The renal vitamin K-dependent gamma-glutamyl carboxylase was present in microsomes prepared from the renal cortex as well as the inner and outer medulla of several species. When glomeruli were isolated from either dog or rat renal cortex, microsomes prepared from these glomeruli were devoid of carboxylase activity. Proximal tubules were isolated from rabbit renal cortex; microsomes derived from these tubules had easily measurable carboxylase activity. The experiments demonstrate that the vitamin K-dependent gamma-glutamyl carboxylase is located in renal tubule cells. Since the enzyme is found not only in cortex but also inner and outer medulla, its distribution is not restricted to specific tubule segments.
TSDC investigations of thermally and electro-thermally aged cable models (2002)

Classical TSD and fractional polarisation analyses were carried out on unaged and aged XLPE cable models from -120/spl deg/C to 120/spl deg/C and three relaxation processes were found. A low temperature process, identified as /spl beta/ relaxation and ascribed to the polar groups attached to the polymer chains in the amorphous region, gets stronger with duration of the aging and can be mainly ascribed to an increase in carbonyl group concentration. The second polarisation process, that exhibits itself by a strong TSD peak at a temperature range 50-80/spl deg/C and is only present in the spectra of aged samples, is believed to be caused by a charge relaxation. The high temperature peak, present above 100/spl deg/C, can be related to melting of crystalline phase in the polymer. This peak should also be related to charge polarisation process. Fractional depolarisation measurements allowed for a precise description of elementary processes (activation energies and preexponential factors).< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Weak Signals Detection and Single Photon Counting with Ultraviolet SiC-based Avalanche Photodiodes (2007)

We report on the responsivity measurement of these devices in the linear regime with quantum efficiency of up to 45%. Responsivity attenuation is significant at wavelengths longer than 400nm and thin film dielectric stack filters may be used to tailor the wavelength response of the SiC APDs for various applications, including those requiring their use in the UV-A, UV-B and UV-C ranges with very good blocking in the visible or solar spectrum.
Nonlinear multigrid inversion for optical diffusion tomography (2005)

A nonlinear multigrid inversion algorithm is presented for solving computationally demanding optimizations in optical diffusion tomography. Simulation results demonstrate the potential for large computational saving, better reconstruction quality, and robust convergence.
High-definition technology of AI inference scheme for object detection on edge/terminal (2023)

To detect a wide range of objects with one camera at once, real-time object detection in high-definition video is required in video artificial intelligence (AI) applications for edge/terminal, such as beyond-visual-line-of-sight (BVLOS) drone flight. Although various AI inference schemes for object detection (e.g., you-only-look-once (YOLO)) have been proposed, they typically have limitations on the input image size and thus need to shrink the input high-definition image down to the limit. This makes small objects collapsed and undetectable. This paper presents our proposal technology for solving this problem and its effective implementation, where multiple object detectors cooperate to detect small and large objects in high-definition video such as full HD and 4K.
Environmental Effects of Nanoceria on Seed Production of Common Bean (Phaseolus vulgaris): A Proteomic Analysis (2015)

The rapidly growing literature on the response of edible plants to nanoceria has provided evidence of its uptake and bioaccumulation, which delineates a possible route of entry into the food chain. However, little is known about how the residing organic matter in soil may affect the bioavailability and resulting impacts of nanoceria on plants. Here, we examined the effect of nanoceria exposure (62.5-500 mg/kg) on kidney bean (Phaseolus vulgaris) productivity and seed quality as a function of soil organic matter content. Cerium accumulation in the seeds produced from plants in organic matter enriched soil showed a dose-dependent increase, unlike in low organic matter soil treatments. Seeds obtained upon nanoceria exposure in soils with higher organic matter were more susceptible to changes in nutrient quality. A quantitative proteomic analysis of the seeds produced upon nanoceria exposure provided evidence for upregulation of stress-related proteins at 62.5 and 125 mg/kg nanoceria treatments. Although the plants did not exhibit overt toxicity, the major seed proteins primarily associated with nutrient storage (phaseolin) and carbohydrate metabolism (lectins) were significantly down-regulated in a dose dependent manner upon nanoceria exposure. This study thus suggests that nanoceria exposures may negatively affect the nutritional quality of kidney beans at the cellular and molecular level. More confirmatory studies with nanoceria along different species using alternative and orthogonal "omic" tools are currently under active investigation, which will enable the identification of biomarkers of exposure and susceptibility.
Post-discharge prognosis of patients admitted to hospital for heart failure by world region, and national level of income and income disparity (REPORT-HF): a cohort study (2020)

Novartis Pharma.
<title>SQL-capable optoelectronic database accelerator for parallel-access optical memories</title> (1998)

The Structured Query Language (SQL) is the de facto standard for commercial database management systems. Optoelectronic database accelerator architectures must be able to support the complex arithmetic functionality of SQL to be accepted in the marketplace. We propose an optoelectronic architecture which directly supports SQL by combining an optically loaded RAM with conventional electronic circuity.
RESIDUE DRUM VENT FILTER MONITORING PROGRAM AT THE ROCKY FLATS ENVIRONMENTAL TECHNOLOGY SITE (1999)

Several thousand 55-gallon drums of residue material from past plutonium processing operations are being stored at the Rocky Flats Environmental Technology Site (RFETS). This stored material awaits stabilization, packaging for shipment to the Waste Isolation Pilot Plant for disposal, or packaging for shipment to other DOE sites for processing. Each of these drums is fitted with a vent filter to prevent accumulation of hydrogen or other flammable gases. A filter-monitoring program was developed to assure that these vent filters continue to function properly. Nuclear Filter Technology Model 012 and 013 filters are being used to vent the drums. A simple filter test device has been developed at RFETS for monitoring these vent filters. This test device consists of a cup that sits over the filter and seals to the drum lid, a pressure gauge, a flow meter, and a hand pump. If the flow rate through the filter at 1-inch water column is less than 35 ml/min, the filter is considered plugged, and the filter is changed. During its first year, the vent-monitoring program has led to discovery of 14 plugged vent filters. These plugged filters were found on drums containing dry combustibles, wet combustibles, and Ful Flo filters. Calculations have shown that these drums could have contained hydrogen gas concentrations in excess of the
Production of Acrylamide using Alginate‐Immobilized E. coli Expressing Comamonas testosteroni 5‐MGAM‐4D Nitrile Hydratase (2005)

Abstract A thermally‐stable nitrile hydratase produced by Comamonas testosteroni 5‐MGAM‐4D has been expressed in Escherichia coli , and the alginate‐immobilized transformant evaluated as catalyst for the conversion of acrylonitrile to acrylamide. In batch reactions with catalyst recycle, the catalyst productivity decreased with increasing acrylonitrile concentration or reaction temperature, but was relatively insensitive to acrylamide concentration. A total of 206 consecutive batch reactions with catalyst recycle were run at 5 °C and produced 1035 g acrylamide/g dry cell weight and 95 g acrylamide/L; the initial and final volumetric productivities of this series of recycle reactions were 197 and 96 g acrylamide/L/h, respectively. A packed column reactor yielded lower catalyst productivity than consecutive batch recycle reactions, presumably due to contact of the fixed‐bed catalyst with a constant high concentration of acrylonitrile.
Developments in acoustics and ultrasonics : proceedings of the meeting organised by the IOP Physical Acoustics Group, Leeds, UK, 24-25 September 1991 (1992)

New directions in ultrasound, M.J.W. Povey propagation and attenuation of ultrasound in suspensions, A.H. Harker and J.A.G. Temple ultrasound propagation in heterogeneous systems, R.C. Chivers two aspects of the use of acoustics in the quantitative assessment of fish stocks, C. Javanaud broad band techniques for the ultrasonic study of materials, R.E. Challis the Doppler effect and blood flow - an instrumentation optimization programme, P.J. Fish what do ultrasound measurements in fruit and vegetables tell you?, G.K. Self, et al developments of ultrasound in the study of foods, D.J. McClements ultrasonic measurements of high Tc superconductors, D.P. Almond modelling of sea-bed classification systems, R.C. Chivers and C.G. Meenan ultrasonic studies of rare earth phosphate glasses, H.B. Senin, et al ultrasonic investigation of magnetic rare earth layers, G. Akuety, et al the longitudinal and the shear ultrasonic velocities in thin samples of AIN, A12O3 and SiC ceramics, T. Alper, et al deep subsurface imaging using shear wave mode acoustic microscopy, D. Zhang and G.M. Crean non-linear acoustic properties of high purity quartz as a function of temperature, Q. Wang, et al vibrations of an annular membrane, R.C. Chivers, et al improved signal processing for ultrasonic propagation parameter determination, W. Boek, et al an ultrasonic investigation of aerated cream, P. Fairley, et al ultrasonic studies of aqueous salt solutions of a globular protein, G. Pavlovskaya, et al attenuation studies in yeast culture, Pankaj and R.C. Chivers ultrasonic characterization of cereal stem tissues, J. Zebrowski.
Near-Field Enhanced Photochemistry of Single Molecules in a Scanning Tunneling Microscope Junction (2017)

Optical near-field excitation of metallic nanostructures can be used to enhance photochemical reactions. The enhancement under visible light illumination is of particular interest because it can facilitate the use of sunlight to promote photocatalytic chemical and energy conversion. However, few studies have yet addressed optical near-field induced chemistry, in particular at the single-molecule level. In this Letter, we report the near-field enhanced tautomerization of porphycene on a Cu(111) surface in a scanning tunneling microscope (STM) junction. The light-induced tautomerization is mediated by photogenerated carriers in the Cu substrate. It is revealed that the reaction cross section is significantly enhanced in the presence of a Au tip compared to the far-field induced process. The strong enhancement occurs in the red and near-infrared spectral range for Au tips, whereas a W tip shows a much weaker enhancement, suggesting that excitation of the localized plasmon resonance contributes to the process. Additionally, using the precise tip-surface distance control of the STM, the near-field enhanced tautomerization is examined in and out of the tunneling regime. Our results suggest that the enhancement is attributed to the increased carrier generation rate via decay of the excited near-field in the STM junction. Additionally, optically excited tunneling electrons also contribute to the process in the tunneling regime.
Acoustics of a Supersonic Cavity with a Generic Store (2015)

The paper presents a comprehensive study involving experiments and numerical simulations to measure the acoustic field in a rectangular supersonic cavity with and without a generic store. Unsteady pressure spectra measured in the empty cavity show the presence of highamplitude, discrete tones at all measurement locations with highest fluctuation levels at the downsteam wall of the cavity. The frequency of cavity tones is well predicted by Rossiter modes of resonance. Pressure fluctuation levels increase with Reynolds number and assumes a universal character when non-dimensionalized with freestream dynamic pressure. In the presence of a store, not only the resonance modes in the cavity are altered but the energy is redistributed among various modes. A reasonable correlation is observed between CFD and experimental data.
Integrin Receptor Activation Triggers Converging Regulation of Cav1.2 Calcium Channels by c-Src and Protein Kinase A Pathways (2006)

L-type, voltage-gated Ca2+ channels (CaL) play critical roles in brain and muscle cell excitability. Here we show that currents through heterologously expressed neuronal and smooth muscle CaL channel isoforms are acutely potentiated following alpha5beta1 integrin activation. Only the alpha1C pore-forming channel subunit is critical for this process. Truncation and site-directed mutagenesis strategies reveal that regulation of Cav1.2 by alpha5beta1 integrin requires phosphorylation of alpha1C C-terminal residues Ser1901 and Tyr2122. These sites are known to be phosphorylated by protein kinase A (PKA) and c-Src, respectively, and are conserved between rat neuronal (Cav1.2c) and smooth muscle (Cav1.2b) isoforms. Kinase assays are consistent with phosphorylation of these two residues by PKA and c-Src. Following alpha5beta1 integrin activation, native CaL channels in rat arteriolar smooth muscle exhibit potentiation that is completely blocked by combined PKA and Src inhibition. Our results demonstrate that integrin-ECM interactions are a common mechanism for the acute regulation of CaL channels in brain and muscle. These findings are consistent with the growing recognition of the importance of integrin-channel interactions in cellular responses to injury and the acute control of synaptic and blood vessel function.
Characteristic Experiments of Linear MR Brake for Intelligent Prosthetic Ankle Joint (2003)

本研究は, 義足足部の関節を制御するための直動型MRブレーキの開発・制御を目的とする。本紙では製作した直動型MRブレーキの特性実験の結果を示す。