gebhart/patrepeval huggingface dataset:
Distribution of furamidine analogues in tumor cells: targeting of the nucleus or mitochondria depending on the amidine substitution. (2002)

Diphenylfuran diamidines represent an important class of DNA minor groove binders of high therapeutic interest as antiparasitic or antitumor agents depending on the compounds structures. To exert their cytotoxic action, the compounds must first get into the cell and reach the nuclear compartment where the main target, DNA, is located. The forces that drive the drugs into cell nuclei, as well as the influence of the molecular structures on the cell distribution, are not known. To address these issues, we took advantage of the fluorescence of the molecules to analyze their intracellular distribution profiles in tumor cells of different origins (B16 melanoma, MCF7 mammary adenocarcinoma, A549 lung carcinoma, HT29 colon carcinoma, LNCaP, and PC3 prostatic carcinoma) by epifluorescence and confocal microscopy. A homogeneous series of synthetic bis-substituted alkyl or phenyl amidine and reverse amidine derivatives of furamidine was used to dissect the molecular mechanisms that control the distribution of the drugs into the cytoplasm or the nucleus of the cells. The amidine (DB75) and the various N-alkyl derivatives were found to accumulate selectively in the cell nuclei. This is also the case for a guanidine derivative but not for the phenyl-substituted compound DB569, which essentially localizes in cytoplasmic granules. Similar cytoplasmic patterns were observed with a reverse amidine analogue and a pyridine-substituted compound indicating that the presence of aromatic rings on the terminal side chain is the limiting factor that restricts the uptake of the compounds in the nuclear compartment. The use of different organelle-selective fluorescent probes, such as JC-1 and chloromethyl-X-rosamine, both specific to mitochondria and neutral red considered as a lysosome-selective probe, suggests that DB569 preferentially accumulates in mitochondria. Competition experiments with the antitumor drug daunomycin reveal that the diphenylfurans are attracted into the nuclei by the DNA. The DNA minor groove-drug interactions provide the driving force that permits massive accumulation of the fluorescent molecules in the nuclei. The DNA binding properties of the diphenylfuran derivatives were investigated by DNase I footprinting and surface plasmon resonance biosensor experiments to measure sequence selectivity and binding affinities, respectively. Furamidine and its phenyl-substituted analogue that accumulate in the cell nuclei and mitochondria, respectively, share a common selectivity for AT sites and bind equally tightly to these sites. Therefore, it is possible to modulate the intracellular distribution of the furamidine derivatives without affecting their DNA binding and sequence recognition properties. The introduction of aromatic substituents on diphenylfuran diamidines represents a novel strategy to control the intracellular compartmentalization of these DNA binding agents and directs them to mitochondria. This drug design strategy may prove useful to trigger drug-induced apoptosis.
A Computational Pipeline for Patient-Specific Prediction of the Postoperative Mitral Valve Functional State (2023)

While mitral valve (MV) repair remains the preferred clinical option for mitral regurgitation (MR) treatment, long-term outcomes remain suboptimal and difficult to predict. Furthermore, pre-operative optimization is complicated by the heterogeneity of MR presentations and the multiplicity of potential repair configurations. In the present work, we established a patient-specific MV computational pipeline based strictly on standard-of-care pre-operative imaging data to quantitatively predict the post-repair MV functional state. First, we established human mitral valve chordae tendinae (MVCT) geometric characteristics obtained from five CT-imaged excised human hearts. From these data, we developed a finite-element model of the full patient-specific MV apparatus that included MVCT papillary muscle origins obtained from both the in vitro study and the pre-operative three-dimensional echocardiography images. To functionally tune the patient-specific MV mechanical behavior, we simulated pre-operative MV closure and iteratively updated the leaflet and MVCT prestrains to minimize the mismatch between the simulated and target end-systolic geometries. Using the resultant fully calibrated MV model, we simulated undersized ring annuloplasty (URA) by defining the annular geometry directly from the ring geometry. In three human cases, the postoperative geometries were predicted to 1 mm of the target, and the MV leaflet strain fields demonstrated close agreement with noninvasive strain estimation technique targets. Interestingly, our model predicted increased posterior leaflet tethering after URA in two recurrent patients, which is the likely driver of long-term MV repair failure. In summary, the present pipeline was able to predict postoperative outcomes from pre-operative clinical data alone. This approach can thus lay the foundation for optimal tailored surgical planning for more durable repair, as well as development of mitral valve digital twins.
Zeolite Synthesis: Can It Be Designed? (1994)

The possibility of designing zeolites by their controlled synthesis is discussed. The feasibility of creating pore architectures through the design of organic structure-directing agents is described for high-silica zeolites. The first example of such a design is presented. A strategy for building pure-silica zeolites from molecular precursors is outlined. It is concluded that the total design of zeolite structures through their designed synthesis is not currently obtainable but information providing "pieces to this puzzle" are quickly being assembled.
Cartilage tissue engineering with novel nonwoven structured biomaterial based on hyaluronic acid benzyl ester (1998)

The aim of this study was to investigate the possibility of using the benzyl ester of hyaluronic acid (HYAFF® 11), a recently developed semisynthetic resorbable material, as a scaffold for the culture of human nasoseptal chondrocytes in tissue-engineering procedures of cartilage reconstruction. Different techniques such as immunohistochemistry, scanning electron microscopy, and confocal laser scanning microscopy were used to study the behavior, morphology, and phenotype expression of the chondrocytes, which were initially expanded and then seeded on the material. The nonwoven cell carrier allowed good viability and adhesivity of the cells without any surface treatment with additional substances. Furthermore, the cultured cells expressed cartilage-specific collagen type II, indicating that they were able to redifferentiate within the scaffold of HYAFF® 11 and were able to retain a chondrocyte phenotype even after a long period of in vitro conditions. Nevertheless, the expression of collagen type I, which was produced by dedifferentiated or incompletely redifferentiated chondrocytes, was noticeable. Additional data were obtained by subcutaneous implantation of samples seeded with human cells in the in vivo model of the athymic nude mouse. The results after 1 month revealed the development of tissue similar to hyaline cartilage. This study is promising for the use of this scaffold for tissue engineering of cartilage replacements. © 1998 John Wiley & Sons, Inc. J Biomed Mater Res, 42, 172–181, 1998.
PROPERTIES OF GALACTIC CIRRUS CLOUDS OBSERVED BY BOOMERANG (2010)

The physical properties of galactic cirrus emission are not well characterized. BOOMERANG is a balloon-borne experiment designed to study the cosmic microwave background at high angular resolution in the millimeter range. The BOOMERANG 245 and 345 GHz channels are sensitive to interstellar signals, in a spectral range intermediate between FIR and microwave frequencies. We look for physical characteristics of cirrus structures in a region at high galactic latitudes (b ~ –40°) where BOOMERANG performed its deepest integration, combining the BOOMERANG data with other available data sets at different wavelengths. We have detected eight emission patches in the 345 GHz map, consistent with cirrus dust in the Infrared Astronomical Satellite maps. The analysis technique we have developed allows us to identify the location and the shape of cirrus clouds, and to extract the flux from observations with different instruments at different wavelengths and angular resolutions. We study the integrated flux emitted from these cirrus clouds using data from Infrared Astronomical Satellite (IRAS), DIRBE, BOOMERANG and Wilkinson Microwave Anisotropy Probe in the frequency range 23-3000 GHz (13 mm-100 μm wavelength). We fit the measured spectral energy distributions with a combination of a gray body and a power-law spectra considering two models for the thermal emission. The temperature of the thermal dust component varies in the 7-20 K range and its emissivity spectral index is in the 1-5 range. We identified a physical relation between temperature and spectral index as had been proposed in previous works. This technique can be proficiently used for the forthcoming Planck and Herschel missions data.
ChemInform Abstract: β‐Lactams from Esters and Sulfenimines: A New Route to Monobactams. (1986)

Abstract Die Sulfenimine (I) reagieren mit den aus den Estern (II), (IV) bzw. (VII) hergestellten Esterenolaten zu den β‐Lactamen (IIIa), (V) und (VI) bzw. (VIII).
Spotlight: Scalable Transport Layer Load Balancing for Data Center\n Networks (2018)

Load Balancing plays a vital role in modern data centers to distribute\ntraffic among instances of network functions or services. State-of-the-art load\nbalancers such as Silkroad dispatch traffic obliviously without considering the\nreal-time utilization of service instances and therefore can lead to uneven\nload distribution and suboptimal performance.\n In this paper, we design and implement Spotlight, a scalable and distributed\nload balancing architecture that maintains connection-to-instance mapping\nconsistency at the edge of data center networks. Spotlight uses a new stateful\nflow dispatcher which periodically polls instances' load and dispatches\nincoming connections to instances in proportion to their available capacity.\nOur design utilizes distributed control plane and in-band flow dispatching and\nthus scales horizontally in data center networks. Through extensive flow-level\nsimulation and packet-level experiments on a testbed, we demonstrate that\ncompared to existing methods Spotlight distributes the traffic more efficiently\nand has near-optimum performance in terms of overall service utilization.\nMoreover, Spotlight is not sensitive to utilization polling interval and\ntherefore can be implemented with low polling frequency to reduce the amount of\ncontrol traffic. Indeed, Spotlight achieves the mentioned performance\nimprovements using O(100ms) polling interval.\n
PNGV Superplastic Forming of Stainless Steel Auto Components (USCAR) Final Report CRADA No. TC-0881-94 (1998)

The DOE/DP CRADA consisted of three tasks that were to be performed concurrently by LMES and LLNL. LMES evaluated mechanical properties of candidate duplex stainless steel alloys at various temperatures and strain rate to determine forming behavior. LLNL evaluated the weldability and the effect of the heat affected zone (HAZ) from the weld on the microstructure of duplex stainless steel. At the beginning of the CRADA, commercially available duplex stainless steel was purchased to provide material that would have optimum characteristics. Superdux 65 (SD65) was acquired and tested and was used to form initial test parts. LMES cast and processed a couple of duplex stainless steel alloys that exhibited superplastic forming (SPF) behavior in case a domestic material was unavailable.
Polarized light spatial frequency domain imaging for non-destructive quantification of soft tissue fibrous structures (2015)

The measurement of soft tissue fiber orientation is fundamental to pathophysiology and biomechanical function in a multitude of biomedical applications. However, many existing techniques for quantifying fiber structure rely on transmitted light, limiting general applicability and often requiring tissue processing. Herein, we present a novel wide-field reflectance-based imaging modality, which combines polarized light imaging (PLI) and spatial frequency domain imaging (SFDI) to rapidly quantify preferred fiber orientation on soft collagenous tissues. PLI utilizes the polarization dependent scattering property of fibers to determine preferred fiber orientation; SFDI imaging at high spatial frequency is introduced to reject the highly diffuse photons and to control imaging depth. As a result, photons scattered from the superficial layer of a multi-layered sample are highlighted. Thus, fiber orientation quantification can be achieved for the superficial layer with optical sectioning. We demonstrated on aortic heart valve leaflet that, at spatial frequency of f = 1mm(-1) , the diffuse background can be effectively rejected and the imaging depth can be limited, thus improving quantification accuracy.
Spatial and Temporal Control of Transgene Expression Through Ultrasound-Mediated Induction of the Heat Shock Protein 70B Promoter In Vivo (2002)

Adenoviral vectors have been constructed that express the transgenes luciferase (Adeno-HSP-Luc) or Fas ligand (Adeno-HSP-FasL) under the control of the heat shock protein 70B (hsp70B) promoter. Cultures infected with Adeno-HSP-Luc transiently expressed high levels of luciferase after heat shock. When cultures infected with Adeno-HSP-FasL were maintained at 37 degrees C, no transgene expression was observed, but when cultures were exposed to heat stress, transgene expression resulted in apoptotic cell death. In vivo, transgene expression was induced by ultrasound-mediated heating of adenovirus-infected tissue. In mice or rats injected with the Adeno-HSP-Luc construct, high levels of localized expression of luciferase activity were observed in regions subjected to ultrasound-mediated irradiation. Adeno-HSP-FasL was administered systemically to mice via the tail vein to evaluate safety. Animals receiving Adeno-HSP-FasL in the absence of ultrasound treatment did not display liver toxicity, whereas animals receiving ultrasound treatment to induce the expression of Fas ligand from the hsp70B promoter had significant increases in serum levels of liver enzymes. These data demonstrate that combining the inducible hsp70B promoter with ultrasound induction allows safe local expression of cytotoxic genes with possible therapeutic utility.
Application of a MIMO-PID Controller for a Hydraulic Excavator Considering the Velocity of CoM (2020)

There are many machines that require human operation in industry, and high operational skills are required to operate these machines efficiently. However, the number of highly skilled workers is decreasing due to the recent trends of falling birthrate and population aging. This decline is particularly pronounced in the construction industry, while the demand for construction workers remains high owing to the increasing number of developed infrastructures. To reduce this mismatch between the supply and the demand, it is important to achieve high efficiency in tasks using hydraulic excavators, because these machines can greatly increase the productivity at construction sites. Accordingly, it is necessary to improve productivity even if unskilled operators use hydraulic excavators. This paper proposes a control system that achieves efficient motions based on the velocity of the center of mass (CoM) of the hydraulic excavator’s attachments, which reflects the characteristics of skilled workers’ operations. The motions of multiple attachments give rise to interference terms owing to the characteristics of the hydraulic system. A two-input two-output control system, in which the input consists of the lever input and the output is the CoM velocity is constructed. The fictitious reference iterative tuning (FRIT) method is used to calculate the controller parameters. The proposed method was verified by comparing the results of a simulated digging motion and an experiment with an actual hydraulic excavator operated by an unskilled operator.
Selective Synchronization of Tetrahymena pyriformis Cell Populations and Cell Growth Kinetics during the Cell Cycle (1998)

A selection synchronization technique based on ingestion of tantalum particles has been applied to obtain synchronized cultures of the filter feeding ciliate Tetrahymena pyriformis. Cell concentrations and cell volume distributions of synchronized cell populations have been monitored for more than four average generation times. A simple curve-fitting method has been used to decompose the cell volume distributions of a synchronous population into two populations representing cells before and after cell division. In this way, the time course of the growth of an initially synchronous culture is decomposed into the growth of successive generations. The data indicate that at any given time only two generations of cells are present in significant numbers. The measured cell volume distributions show that T. pyriformis has a complicated growth pattern during the cell cycle. Newborn T. pyriformis cells do not grow significantly at the beginning of the cell cycle. After the nongrowing stage, cells start growing in a possibly exponential rate before cells enter into a second nongrowing stage. The second nongrowing stage lasts until cell division. The presented data demonstrate that growing cell populations can be viewed as the composite of cells belonging to different generations. This concept has important implications for solving corpuscular models of cell growth.
Recent advances in broadcast audio coding (2013)

In the last decades there have been major advances in broadcasting technologies as well as a change in consumer behaviour and demands. The on-demand concepts and streaming technology, together with widely available fixed and mobile broadband Internet connections, had an eminent impact on the broadcasters to fit the consumer's needs with respect to content variety, quality and availability. Due to the limited capacity available for broadcasting content, especially over the air, more and more efficient codecs were necessary. This paper will give a short overview of the history of broadcast audio coding and point out which codecs are currently used in the major broadcasting systems, i.e. DVB, DAB and DRM. Recent developments in broadcast audio coding will be presented, focusing on spatial audio object coding and unified speech and audio coding technologies.
Hyperthermia inhibits angiogenesis by a plasminogen activator inhibitor 1-dependent mechanism. (2003)

Hyperthermia (HT) associated with radiotherapy or chemotherapy is a promising method for cancer treatment, although the molecular mechanisms of this process are not well understood. HT exhibits various antitumor effects, including damage of tumor vasculature. Here, we investigate the effect of HT on in vitro and in vivo angiogenesis. We show that heat treatment of endothelial cells (ECs) affect their differentiation into capillary-like structures in two models of in vitro angiogenesis. Furthermore, the formation of new vessels promoted by angiogenic inducers in the chick embryo chorioallantoic membrane assay is impaired after heat treatment. These effects cannot be explained by direct cytotoxicity but are dependent on modulation of angiogenesis-involved genes. Gene expression profile of ECs subjected to heat shock demonstrates that plasminogen activator inhibitor 1 (PAI-1), a protein involved in the control of extracellular matrix degradation, is specifically up-regulated. The use of anti-PAI-1-neutralizing antibodies reverts the effect of HT on the in vitro EC morphogenesis and in vivo vessel formation. Moreover, microvessel outgrowth from PAI-1(-/-) aortic rings was not affected by HT compared with aortic rings from PAI-1(+/+) mice. Heat treatment of murine mammary adenocarcinomas results in inhibition of tumor growth, associated with a reduction of microvessel number and an increase of PAI-1 expression. These results indicate that heat-mediated PAI-1 induction is an important pathway by which HT exerts its antitumor activity and may represent a rationale for a combined cancer therapy based on HT associated with antiangiogenic molecules.
Joint Constellation Rotation and Symbol-level Precoding Optimization in the Downlink of Multiuser MISO Channels (2020)

This paper tackles the problem of the simultaneous interference among the multiple users in the downlink of a wireless multiantenna system. In order to exploit the multiuser interference and transform it into useful power at the receiver side, symbol-level precoding is utilized through joint exploitation of the data from each individual user and the channel state information. This paper introduces a complexity reduction scheme that exploits the constellations symmetry to reduce the number of possible calculations and the number of constraints in the symbol-level optimization problem. This paper also proposes a new transmission technique that jointly optimizes the transmit symbol-level precoding and the constellation rotation of the data stream for each user. The purpose is to increase the probability of having constructive interference, and thus better performance. We focus on improving the energy efficiency by reducing the required power to satisfy certain quality of service constraints through solving a symbol-level precoding problem and finding the optimal phase by which each user constellation should be rotated. This problem is non-convex, therefore, we propose an efficient branch-and-bound algorithm combined with semidefinite relaxation to solve the problem and find an efficient solution. Numerical results are presented in a comparative fashion to show the effectiveness of the proposed technique, which outperforms the state-of-the-art symbol-level precoding schemes in terms of energy efficiency, and power consumption.
Sub-micrometer resolution neutron tomography (2018)

We demonstrate a neutron tomography technique with sub-micrometer spatial resolution. Our method consists of measuring neutron diffraction spectra using a double crystal diffractometer as a function of sample rotation and then using a phase retrieval algorithm followed by tomographic reconstruction to generate a density map of the sample. In this first demonstration, silicon phase-gratings are used as samples, the periodic structure of which allows the shape of the gratings to be imaged without the need of position sensitive detectors. Topological features found in the reconstructions also appear in scanning electron micrographs. The reconstructions have a resolution of about 300 nm, which is over an order of magnitude smaller than the resolution of radiographic, phase contrast, differential phase contrast, and dark field neutron tomography methods. Further optimization of the underlying phase recovery and tomographic reconstruction algorithm is also considered.
Equatorial ring-like channels in theCryptococcus neoformanspolysaccharide capsule (2006)

Under certain conditions, India ink particles can penetrate the capsule of the opportunistic pathogen Cryptococcus neoformans. India ink penetration gave two distinct patterns, one as a ring in the middle of the capsule, and another as a double spot located at opposite poles of the cells. These spots were perpendicularly orientated to the bud. This pattern suggests the existence of a localized structure deep in the capsule that can accumulate large insoluble particles. Although the mechanisms responsible for the assemblage and maintenance of ring-like channels are not understood, their existence deep within the capsule implies a new level of complexity for this enigmatic structure.