gebhart/patrepeval huggingface dataset:
A Potential Role for the Myeloid Lineage in Leptin-regulated Bone Metabolism (2011)

Leptin influences bone formation centrally through the hypothalamus and peripherally by acting on osteoblasts or their precursors. However, neither mechanism explains the divergent, gender-specific correlation between leptin and bone mineral density in humans. Although leptin is a potent regulator of pro-inflammatory immune responses, a potential role for leptin as an osteoimmunologic intermediate in bone metabolism has not been tested. Mice with myeloid-specific ablation of the long-form leptin receptor (ObRb) were generated using mice expressing cre-recombinase from the lysoszyme M promoter. At 12 weeks of age, the conditional knockout mice did not display any appreciable phenotype. However, at 52 weeks 2 changes were noted. First, there was a mild increase in liver inflammation. Second, a gender-specific, divergent bone phenotype was observed. Female mice displayed a consistent trend toward decreased trabecular bone parameters including reductions in bone volume fraction, trabecular number, and bone mineral content as well as a significant increase in marrow adipogenesis. Conversely, male mice lacked trabecular changes, but had statistically significant increases in cortical bone volume, thickness, and bone mineral density with equivalent total cortical volume. Since the year 2000, over 25 studies on more than 10,000 patients have sought to determine the correlation between leptin and bone mineral density. The results revealed a gender-specific correlation similar to that observed in our LysM transgenic animals. We hypothesize and show new evidence that regulation of myeloid lineage cells by leptin may facilitate their actions as an osteoimmunologic intermediate and contribute to leptin-regulated bone formation and metabolism in a gender-specific manner.
The function of the calcium channel Orai1 in osteoclast development (2021)

To determine the intrinsic role of Orai1 in osteoclast development, Orai1-floxed mice were bred with LysMcre mice to delete Orai1 from the myeloid lineage. PCR, in situ labelling and Western analysis showed Orai1 deletion in myeloid-lineage cells, including osteoclasts, as expected. Surprisingly, bone resorption was maintained in vivo, despite loss of multinucleated osteoclasts; instead, a large number of mononuclear cells bearing tartrate resistant acid phosphatase were observed on cell surfaces. An in vitro resorption assay confirmed that RANKL-treated Orai1 null cells, also TRAP-positive but mononuclear, degraded matrix, albeit at a reduced rate compared to wild type osteoclasts. This shows that mononuclear osteoclasts can degrade bone, albeit less efficiently. Further unexpected findings included that Orai1<sup>fl/fl</sup> -LysMcre vertebrae showed slightly reduced bone density in 16-week-old mice, despite Orai1 deletion only in myeloid cells; however, this mild difference resolved with age. In summary, in vitro analysis showed a severe defect in osteoclast multinucleation in Orai1 negative mononuclear cells, consistent with prior studies using less targeted strategies, but with evidence of resorption in vivo and unexpected secondary effects on bone formation leaving bone mass largely unaffected.
Randomized control trials using a tablet formulation of hyperimmune bovine colostrum to prevent diarrhea caused by enterotoxigenicEscherichia coliin volunteers (2011)

Active tablet formulations were significantly more effective than placebo in protecting volunteers against the development of diarrhea caused by ETEC. These results suggest that administration of a tablet formulation of hyperimmune bovine colostrum containing antibodies against ETEC strains may reduce the risk of travelers' diarrhea.
Nonlinear S-Parameters Inversion for Stroke Imaging (2020)

Stroke identification by means of microwave tomography requires a very accurate reconstruction of the dielectric properties inside patient's head. This is possible when a precise measurement system is combined with a full nonlinear inversion method. In this article, the inversion of S-parameter data collected in a metallic chamber is performed with a nonlinear inversion strategy in Lebesgue spaces with nonconstant exponents. This is the first time that this kind of nonlinear S-parameter electromagnetic formulation has been applied to this problem. The inverse-scattering method incorporates a 2-D electromagnetic model of the imaging chamber based on a finite-element formulation, which has led to a complete redefinition of the solving procedure with respect to previous works. This allows a suitable description of the multistatic S-parameters due to the interactions between the incident radiation and the structure under test. The developed inversion procedure is first assessed by means of numerical simulations. The experimental results, obtained with a clinical microwave system prototype containing a liquid-filled 3-D SAM phantom with an inhomogeneity mimicking a hemorrhagic stroke, further prove the effectiveness of the proposed approach.
Fabrication of Inverted Trapezoidal Microstructures for Heat Transfer and Microfluidics Applications (2014)

In the present work, a novel fabrication technique of inverted trapezoidal microstructures on a silicon substrate is presented. The novel microstructures caused the inherently hydrophilic silicon surface to become hydrophobic without the use of coating or thin film deposition. The microstructures were fabricated on a Silicon On Insulator (SOI) wafer through steps of photolithography, dry/wet etching, and bounding. Starting with a (100) plane SOI wafer, squares were patterned into a nitrite etch mask. Potassium hydroxide (KOH) anisotropic wet etchant was then used to etch the wafer, as a result created pits with flat sloping {111}-oriented sidewalls and a flat (100)-oriented bottom of the silicon device layer (i.e., at SiO2 layer). To get the inverted trapezoids, wafer bonding was carried out after etching, and the handle wafer along with the SiO2 layer were completely etched to expose the small openings of the trapezoids. SEM images were taken to investigate the surface and to measure the dimensions of the square openings from both sides before and after bonding. Contact angle measurements demonstrated the ability of the novel surfaces to form Cassie wetting states with a liquid droplet, sustaining large contact angles above 90 degrees, whereas bare silicon surfaces were measured to have contact angles of around 40 degrees. Shifts in contact angles were attributed to air entrapment.
RNAs Regulate Biology (2006)

ADVERTISEMENT RETURN TO ISSUEPREVIn FocusNEXTRNAs Regulate BiologyRachel Green†‡* and Jennifer A. Doudna†§*View Author Information† Howard Hughes Medical Institute‡ Department of Molecular Biology and Genetics, Johns Hopkins School of Medicine, Baltimore, Maryland 21205§ Department of Molecular and Cell Biology and Department of Chemistry, University of California, Berkeley, California 94720*Corresponding authors, [email protected], [email protected]Cite this: ACS Chem. Biol. 2006, 1, 6, 335–338Publication Date (Web):July 21, 2006Publication History Received29 June 2006Accepted29 June 2006Published online21 July 2006Published inissue 1 July 2006https://pubs.acs.org/doi/10.1021/cb600277mhttps://doi.org/10.1021/cb600277mnewsACS PublicationsCopyright © 2006 American Chemical SocietyRequest reuse permissionsArticle Views435Altmetric-Citations3LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Biology,Genetics,Genomics,Molecular structure,Peptides and proteins Get e-Alerts
Table S1 from BET Inhibitors Suppress ALDH Activity by Targeting <i>ALDH1A1</i> Super-Enhancer in Ovarian Cancer (2023)

&lt;p&gt;Probe and primer sequences for 3C&lt;/p&gt;
Analysis of high-speed corona micromotors by means of effective gap conductivity (2002)

The nonlinear electrical characteristics of corona motors are modeled through suitable transformation of the stator voltage waveform. This in effect produces an equivalent gap conductivity to serve as a linearized model. The analysis shows how a corona motor is able to generate very high speeds with DC excitation, and points out the basis for its asynchronous operation. Experimental tests of a macroscopic motor confirm that the analysis models the basic behavior of a corona machine. Operation of a small (100 mu m) cylindrical motor is considered. The device expected to be capable of very high speeds with DC input and no special control.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Numerical Study on the Performance of a Cogeneration System of Solid Oxide Fuel Cell Based on Biomass Gasification (2021)

In order to solve the environmental pollution problem caused by winter heating of rural residential building in northern of China, in this paper a biomass gasification (BG)-solid oxide fuel cell (SOFC) combined heat and power (CHP) system has been establishedand numerically investigated. Taking a rural village around Xi’an which is an ancient city and located at central of northern China as the study object, according to heat and electricity output of the system and the heating and electrical load characteristics of the residential building of village, the energy saving ratio and economical efficiency of the CHP system under three different operation schemes compared with the traditional energy system have been analyzed. The results show that the operation scheme for heating designated rooms in rural buildings and meeting the average heat demand of users is the most energy-efficient and economical way. The primary energy saving rate and annual cost saving rate can reach 18.0% and 10.3%, respectively. When the user’s heat and power load demand is clear, the closer the system’s output heat and power ratio to the user’s heat and power load ratio, the more significant the system’s energy saving effect.
Xeno-free cryopreservation of adherent retinal pigmented epithelium yields viable and functional cells in vitro and in vivo (2021)

Age-related macular degeneration (AMD) is the primary cause of blindness in adults over 60 years of age, and clinical trials are currently assessing the therapeutic potential of retinal pigmented epithelial (RPE) cell monolayers on implantable scaffolds to treat this disease. However, challenges related to the culture, long-term storage, and long-distance transport of such implants currently limit the widespread use of adherent RPE cells as therapeutics. Here we report a xeno-free protocol to cryopreserve a confluent monolayer of clinical-grade, human embryonic stem cell-derived RPE cells on a parylene scaffold (REPS) that yields viable, polarized, and functional RPE cells post-thaw. Thawed cells exhibit ≥ 95% viability, have morphology, pigmentation, and gene expression characteristic of mature RPE cells, and secrete the neuroprotective protein, pigment epithelium-derived factor (PEDF). Stability under liquid nitrogen (LN<sub>2</sub>) storage has been confirmed through one year. REPS were administered immediately post-thaw into the subretinal space of a mammalian model, the Royal College of Surgeons (RCS)/nude rat. Implanted REPS were assessed at 30, 60, and 90 days post-implantation, and thawed cells demonstrate survival as an intact monolayer on the parylene scaffold. Furthermore, immunoreactivity for the maturation marker, RPE65, significantly increased over the post-implantation period in vivo, and cells demonstrated functional attributes similar to non-cryopreserved controls. The capacity to cryopreserve adherent cellular therapeutics permits extended storage and stable transport to surgical sites, enabling broad distribution for the treatment of prevalent diseases such as AMD.
Substitution and Hydrogenation Reactions on Rhodium(I)−Ethylene Complexes of the Hydrotris(pyrazolyl)borate Ligands Tp‘ (Tp‘ = Tp, TpMe2) (1999)

The bis(ethylene) Rh species TpMe2Rh(C2H4)2(1*) (TpMe2 = tris(3,5-dimethyl-1-pyrazol-1-yl)hydroborate) has been obtained from [RhCl(C2H4)2]2 and KTpMe2. Complex 1* easily decomposes in solution to give mainly the butadiene species TpMe2Rh(eta74-C4H6). In the solid state its thermal decomposition follows a different course and the allyl TpMe2RhH(syn-C3H4Me) is cleanly obtained as a mixture of exo and endo isomers. The complexes Tp'Rh(C2H4)2 (Tp' = Tp, TpMe2) afford the monosubstituted species Tp'Rh(C2H4)(PR3) upon reaction with PR3 but react differently with L = CO or CNR: the Tp compound gives dinuclear [TpRh]2(mu-L)3 complexes, while, in the case of 1*, TpMe2Rh(C2H4)(L) species are obtained. The ethylene ligand of complexes TpMe2Rh(C2H4)(PR3) is labile, and several peroxo compounds of composition TpMe2Rh(O2)(PR3) have been isolated by their reaction with O2. All the mononuclear Rh(I) complexes are formulated as 18e- trigonal bipyramidal species on the basis of IR and NMR spectroscopic studies. A series of dihydride complexes of Rh(III) of formulation Tp'RhH2(PR3) have been prepared by the hydrogenation of the corresponding ethylene derivatives. Complexes [TpRh]2(mu-CNCy)3, TpMe2Rh(C2H4)(PEt3), and TpMe2Rh(O2)(PEt3) have been further characterized by X-ray diffraction studies.
Addressing Missing Labels in Large-Scale Sound Event Recognition Using a Teacher-Student Framework With Loss Masking (2020)

The study of label noise in sound event recognition has recently gained attention with the advent of larger and noisier datasets. This work addresses the problem of missing labels, one of the big weaknesses of large audio datasets, and one of the most conspicuous issues for AudioSet. We propose a simple and model-agnostic method based on a teacher-student framework with loss masking to first identify the most critical missing label candidates, and then ignore their contribution during the learning process. We find that a simple optimisation of the training label set improves recognition performance without additional computation. We discover that most of the improvement comes from ignoring a critical tiny portion of the missing labels. We also show that the damage done by missing labels is larger as the training set gets smaller, yet it can still be observed even when training with massive amounts of audio. We believe these insights can generalize to other large-scale datasets.
Potentiometric Titrations in a Poly(dimethylsiloxane)-Based Microfluidic Device (2004)

This paper describes a microfluidic device, fabricated in poly(dimethylsiloxane), that is used for potentiometric titrations. This system generates step gradients of redox potentials in a series of microchannels. These potentials are probed by microelectrodes that are integrated into the chip; the measured potentials were used to produce a titration curve from which the end point of a reaction was measured.
Masked Spectrogram Modeling using Masked Autoencoders for Learning General-purpose Audio Representation (2022)

Recent general-purpose audio representations show state-of-the-art performance on various audio tasks. These representations are pre-trained by self-supervised learning methods that create training signals from the input. For example, typical audio contrastive learning uses temporal relationships among input sounds to create training signals, whereas some methods use a difference among input views created by data augmentations. However, these training signals do not provide information derived from the intact input sound, which we think is suboptimal for learning representation that describes the input as it is. In this paper, we seek to learn audio representations from the input itself as supervision using a pretext task of auto-encoding of masked spectrogram patches, Masked Spectrogram Modeling (MSM, a variant of Masked Image Modeling applied to audio spectrogram). To implement MSM, we use Masked Autoencoders (MAE), an image self-supervised learning method. MAE learns to efficiently encode the small number of visible patches into latent representations to carry essential information for reconstructing a large number of masked patches. While training, MAE minimizes the reconstruction error, which uses the input as training signal, consequently achieving our goal. We conducted experiments on our MSM using MAE (MSM-MAE) models under the evaluation benchmark of the HEAR 2021 NeurIPS Challenge. Our MSM-MAE models outperformed the HEAR 2021 Challenge results on seven out of 15 tasks (e.g., accuracies of 73.4% on CREMA-D and 85.8% on LibriCount), while showing top performance on other tasks where specialized models perform better. We also investigate how the design choices of MSM-MAE impact the performance and conduct qualitative analysis of visualization outcomes to gain an understanding of learned representations. We make our code available online.
Mechanical rolling and annealing of wire-arc additively manufactured stainless steel plates (2021)

Thin walls of 304L stainless steel were fabricated by wire-arc additive manufacturing (WAAM) followed by post-build thermomechanical processing to modify the as-built microstructure and properties. The walls were made using a high-deposition-rate robotically controlled gas metal arc welding system and were characterised using optical microscopy and tensile tested for mechanical properties. Post-processing consisted of mechanical rolling the walls to 25% and 40% strains, followed by annealing at 1050°C to demonstrate a wide range of properties. Results show that WAAM can be used to build custom-sized semi-finished parts or blanks that can later be thermomechanically processed to increase strength and ductility through recrystallisation of the as-built microstructure, and/or processed into desired shapes with improved mechanical properties.
Efficient memory-reference checks for real-time java (2003)

The scoped-memory feature is central to the Real-Time Specification for Java. It allows greater control over memory management, in particular the deallocation of objects without the use of a garbage collector. To preserve the safety of storage references associated with Java since its inception, the use of scoped memory is constrained by a set of rules in the specification. While a program's adherence to the rules can be partially checked at compile-time, undecidability issues imply that some---perhaps, many---checks may be required at run-time. Poor implementations of those run-time checks could adversely affect overall performance and predictability, the latter being a founding principle of the specification.In this paper we present efficient algorithms for managing scoped memories and the checks they impose on programs. Implementations and results published to date require time linear in the depth of scope nesting; our algorithms operate in constant time. We describe our approach and present experiments quantifying the gains in efficiency.
What Is the Optimal Therapy for Patients with H5N1 Influenza? (2009)

In a 2007 article in PLoS Medicine[10], Holger J. Schünemann and colleagues described a new process used by the World Health Organization for rapidly developing clinical management guidelines in emergency situations. These situations include outbreaks of emerging infectious diseases. The authors discussed how they developed such a "rapid advice" guideline for the pharmacological management of avian influenza A (H5N1) virus infection. The guideline recommends giving the antiviral drug oseltamivir at a dose of 75 mg twice daily for five days. In this Debate, Nicholas White argues that such dosing is inadequate, Robert Webster and Elena Govorkova say that combination antiviral therapy should be used, and Tim Uyeki reminds us that clinical care of patients with H5N1 entails much more than antiviral treatment. These issues may also apply to therapy of patients hospitalized with severe disease due to novel swine-origin influenza A (H1N1) virus infection.
A Novel Factor Required for the Assembly of the DnaK and DnaJ Chaperones of (1996)

We previously reported the isolation of T.DnaK.DnaJ chaperone complex from Thermus thermophilus. Here, we show that a novel factor is necessary for the assembly of T.DnaK and T.DnaJ into the complex. A dnaK gene cluster of T. thermophilus contained five genes, dnaK-grpE-dnaJ-orf4-clpB. Interestingly, T.DnaJ lacks the whole "cysteine-rich region" that has been postulated to be necessary to bind unfolded proteins. The orf4 gene encodes a novel 78-amino acid protein. Curiously, T.DnaK and T.DnaJ expressed in Escherichia coli did not form the complex. Careful reexamination of the T.DnaK.DnaJ complex revealed the presence of a small protein in the complex, which turned out to be a product of orf4. As expected, expression of three genes, dnaK-dnaJ-orf4, resulted in production of a T.DnaK.DnaJ complex in E. coli that was indistinguishable from the authentic complex in its ability to interact with nucleotide and denatured protein. The product of orf4 was also required for in vitro reconstitution of the complex and named T.DafA (T.DnaK.DnaJ assembly factor A). The complex comprises three copies each of T.DnaK, T.DnaJ, and T.DafA. Even though a definite homolog of T.DafA has not been found in the data base, this finding raises a possibility that interaction between DnaK and DnaJ chaperones in other organisms is also mediated by a small protein yet unnoticed.
Multichannel signal modeling and separation (2002)

Separation of multiple signals from their superposition recorded at several sensors is addressed. The methods employ polyspectra of the sensor data in order to extract the unknown signals and estimate the exact coupling systems via a linear equation based method. Proposed schemes are also useful for multichannel blind deconvolution even when the input signals are colored with (possibly) overlapping spectra. Simulation results corroborate the applicability of the algorithm.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>