gebhart/patrepeval huggingface dataset:
919 Tegavivint reduces the immunosuppressive macrophage phenotype in a preclinical co-culture model of the non-small cell lung cancer tumor microenvironment (2021)

<h3>Background</h3> Activation of the Wnt/ß-catenin pathway in non-small cell lung cancer (NSCLC) is associated with tumor growth and metastasis. Activation of this pathway in tumor cells may also modulate the immune tumor microenvironment (TME) by polarizing macrophages into an immunosuppressive M2-like phenotype. We tested whether treatment of co-cultures of human NSCLC cells, cancer-associated fibroblasts (CAFs) and macrophages with Tegavivint (Iterion Therapeutics), a novel compound that targets TBL1 to inhibit ß-catenin function that is in early-phase clinical trials, altered the phenotype of mouse macrophages in vitro. <h3>Methods</h3> Our prior work testing a large panel of patient-derived NSCLC lines in co-cultures of CAFs and mouse bone marrow-derived macrophages (BMDMs) demonstrated that individual NSCLC lines reproducibly generated several macrophage phenotypes, one of which was immunosuppressive (high expression of Arginase-1). We prepared co-cultures of NSCLC cells (H1666 and H2009) (70%), human CAFs (25%) and mouse BMDMs (5%) and demonstrated conversion to the macrophage high Arginase-1 immunosuppressive phenotype by monitoring quantitative expression of mouse genes with qRT-PCR. Expression of ß-catenin protein relative to GAPDH by Western blot was determined before and after treatment with Tegavivint (10 and 20 nM). Co-cultures were treated with Tegavivint (10 nM) for 48 and 72 hours and qRT-PCR for mouse macrophage-specific genes reflecting an immunosuppressive M2-like or an inflammatory, immunostimulatory M1-like phenotype (Arginase-1 and iNos, respectively) was performed. Positive controls consisted of stimulation of macrophages with IL-4 and LPS (to generate M2-like and M1-like phenotypes, respectively). Cytotoxicity assays (MTS and crystal violet) to determine the IC50 of Tegavivint were performed on NSCLC and macrophage cells. <h3>Results</h3> Tegavivint IC50 values for NSCLCs were 17–40 nM. At 10 nM, Tegavivint had no cytotoxic effect on macrophages. Treatment with Tegavivint decreased NSCLC expression of ß-catenin by ~30–40%. When co-cultures of NSCLCs, human CAFs, and mouse BMDMs were treated with 10 nM Tegavivint, we found that macrophage expression of Arginase-1 was significantly inhibited at 48 and 72 hours of treatment (figure 1). <h3>Conclusions</h3> Tegavivint, at pharmacologically achievable doses and concentrations that have little or no cytotoxic effect on NSCLCs or macrophages, decreases tumor cell ß-catenin expression, and reduces the immunosuppressive macrophage phenotype (reduced macrophage Arginase-1 expression) induced by co-cultures of patient-derived NSCLCs and CAFs. These preclinical data set the stage for future clinical translation of Tegavivint as a NSCLC therapeutic in combination with immunotherapy approaches. <h3>Acknowledgements</h3> Supported by P50 CA070907 and Physician-Scientist Institutional Award from the Burroughs Wellcome Fund (TARDIS Fellowship)
Genetic influences on schizophrenia and subcortical brain volumes: Large-scale proof of concept (2016)

Schizophrenia is a devastating psychiatric illness with high heritability. Brain structure and function differ, on average, between people with schizophrenia and healthy individuals. As common genetic associations are emerging for both schizophrenia and brain imaging phenotypes, we can now use genome-wide data to investigate genetic overlap. Here we integrated results from common variant studies of schizophrenia (33,636 cases, 43,008 controls) and volumes of several (mainly subcortical) brain structures (11,840 subjects). We did not find evidence of genetic overlap between schizophrenia risk and subcortical volume measures either at the level of common variant genetic architecture or for single genetic markers. These results provide a proof of concept (albeit based on a limited set of structural brain measures) and define a roadmap for future studies investigating the genetic covariance between structural or functional brain phenotypes and risk for psychiatric disorders.
Asymmetric Catalytic Coupling of Organoboranes, Alkynes, and Imines with a Removable (Trialkylsilyloxy)ethyl Group—Direct Access to Enantiomerically Pure Primary Allylic Amines (2004)

Der Arylsubstituent des Katalysators ist für den Erfolg der Titelreaktion entscheidend (siehe Schema), die mit einem von [Ni(cod)2] und einem P-chiralen Ferrocenylphosphan (z. B. 1) abgeleiteten Katalysator allylische Amine in bis zu 89 % ee und 91 % Ausbeute liefert. Die Kupplungsprodukte lassen sich zu enantiomerenangereicherten, tetrasubstituierten primären allylischen Aminen entschützen, die durch Umkristallisieren optisch rein erhalten werden können.
Global Communication and Memory Optimizing Transformations for Low Power Systems (1994)

Abstract- In this paper we first illustrate the crucialimpact of memory related power consumption on the global system power budget, in particular for multi-dimensional real-time signal processingsubsystems. A realistic medical imaging demonstrator shows that the power budget of the system is clearly dominated by the memory access and that up to an order of magnitude can be gained by transforming the initial specification, even without incorporating the effect of a possible supply voltage reduction. We lhave analyzed the most relevant contributions in this power budget and propose several ways to reduce them. In addition, an automated transformation technique is described to decrease the memory related power budget. LOW POWER SYSTEM DESIGN Many ways exist to realize a given application. The system designer has for instance the choice between many algorithmic procedures for a desired behaviour (e.g. loop reordering). In general, a good trade-off between several characteristics (like power and area) is crucial so there is a rieed for- fast ailcl early feedback alieady ut the algoi-itliin level Mithoiii ahuvs desceridiiig to detailed RTllogic realization
Oscillation Interruption of a Raman Silicon Nanocavity Laser Induced by Positively Ionized-Air Irradiation (2021)

We show that the response of a Raman silicon nanocavity laser to positively ionized-air irradiation is similar to the response observed for negatively ionized air. This demonstrates the potential of such lasers for sensing applications.
In Situ Deprotection and Incorporation of Unnatural Amino Acids during Cell‐Free Protein Synthesis (2013)

The S30 extract from E. coli BL21 Star (DE3) used for cell-free protein synthesis removes a wide range of α-amino acid protecting groups by cleaving α-carboxyl hydrazides; methyl, benzyl, tert-butyl, and adamantyl esters; tert-butyl and adamantyl carboxamides; α-amino form-, acet-, trifluoroacet-, and benzamides; and side-chain hydrazides and esters. The free amino acids are produced and incorporated into a protein under standard conditions. This approach allows the deprotection of amino acids to be carried out in situ to avoid separate processing steps. The advantages of this approach are demonstrated by the efficient incorporation of the chemically intractable (S)-4-fluoroleucine, (S)-4,5-dehydroleucine, and (2S,3R)-4-chlorovaline into a protein through the direct use of their respective precursors, namely, (S)-4-fluoroleucine hydrazide, (S)-4,5-dehydroleucine hydrazide, and (2S,3R)-4-chlorovaline methyl ester. These results also show that the fluoro- and dehydroleucine and the chlorovaline are incorporated into a protein by the normal biosynthetic machinery as substitutes for leucine and isoleucine, respectively.
IPv4 Residual Deployment on IPv6 infrastructure - protocol specification (2011)

This document specifies an automatic tunneling mechanism for providing
IPv4 connectivity service to end users over a service provider's
IPv6 network. Key aspects include stateless operation, sharing of IPv4
addresses, and an algorithmic mapping between IPv4 addresses and IPv6
tunnel endpoints.
Active terahertz time differentiator using piezoelectric micromachined ultrasonic transducer array (2020)

The rapid growth of information technology is closely linked to our ability to modulate and demodulate a signal, whether in the frequency or in the time domain. Recent demonstrations of terahertz (THz) modulation involve active semiconductor metamaterial surfaces or use of a grating-based micromirror for frequency offset tuning. However, a wideband and active differentiator in the THz frequency band is yet to be demonstrated. Here, we propose a simple method to differentiate a THz pulse by inducing tiny phase changes on the THz beam path using a piezoelectric micromachined ultrasonic transducer array. We precisely demonstrate that the modulated THz signal detected after the piezoelectric device is proportional to the first-order derivative of the THz pulse. The proposed technique will be able to support a wide range of THz applications, such as peak detection schemes for telecommunication systems.
Dissociation of Gas Phase Ions of Atomically Precise Silver Clusters Reflects Their Solution Phase Stability (2017)

We report an attempt to probe into the energy demand of the fragmentation of atomically precise silver clusters using collision induced dissociation mass spectrometry. Energy resolved collisions of several gas phase ions of clusters, Ag29(S2R)12, Ag25(SR)18, and Ag44(SR)30, reveal distinct fragmentation kinetics involving charge separation. The fragmentation pattern of [Ag25(SR)18]− is found to be different from its structural analog, [Au25(SR)18]−. Survival yield analysis has been used to establish a direct comparison between the stability of the ions of these clusters, which reveals that [Ag29(S2R)12]3– is the most stable cluster ion, followed by [Ag25(SR)18]− and [Ag44(SR)30]4–. Gas phase stabilities reflect their solution phase stabilities, indicating that the molecular nature of the clusters is retained in the gas phase, too. We further report that fragmentation occurs in a stepwise fashion, conserving the closed shell electronic stability of the parent ion at each step. Such studies are important in understanding the electronic and geometric stability of cluster ions and their fragments.
The Influence of Headphones on the Localization of External Loudspeaker Sources (2015)

When validating systems that use headphones to synthesize virtual sound sources, a direct comparison between virtual and real sources is sometimes needed. This paper considers the passive influence of headphones on the sound transmission and perception of external loudspeaker sources, for which physical measurements and behavioral data have been obtained. Physical measurements of the effect of a number of headphone models are given and analyzed using an auditory filter bank and binaural cue extraction. These highlighted that all of the headphones had an effect on localization cues and repositioning had a measurable effect. A localization test was undertaken using one of the best performing headphones from the measurements. It was found that the presence of the headphones caused a small increase in localization error and that the process of judging source location was different, highlighting a possible increase in the complexity of the localization task.
Enhancement in ordering of FePt films by magnetic field annealing (2004)

Effect of magnetic field annealing on chemical ordering of FePt films has been investigated. It is found that the ordering rate in FePt films is enhanced by applying a magnetic field during postdeposition annealing. Measurements of the structure and magnetic properties of FePt films reveal that the disorder–order transformation starts at or below 450°C when the film is annealed in a magnetic field of 40kOe. The possible reason for the enhancement in the ordering of FePt films by magnetic field annealing is discussed.
Development of Novel DDS Technologies for Pharmacoproteomic-based Drug Discovery and Development (2004)

With the success of the Human Genome Project, the focus of life science research has shifted to the functional and structural analyses of proteins, such as proteomics and structural genomics. These novel approaches to the analysis of proteins, including newly identified ones, are expected to help in the identification and development of protein therapies for various diseases. Thus pharmacoproteomic-based drug discovery currently has a very high profile. Nevertheless, the use of bioactive proteins in the clinical setting is not straightforward because in vivo these proteins have low stability and pleiotropic action. To promote pharmacoproteomic-based drug discovery and development, we have attempted to establish a system for creating functional mutant proteins (muteins) with the desired properties and to develop a site-specific bioconjugation system for further improving their therapeutic potency. These innovative protein-drug systems are discussed in this review.
Dynamics of contour integration (2001)

To determine the dynamics of contour integration the temporal properties of the individual contour elements were varied as well as those of the contour they form. A temporal version of a contour integration paradigm (Field, D. J., Hayes, A., & Hess, R. F. (1993) Vision Research, 33, 173‐193) was used to assess these two temporal dynamics as a function of the contrast of individual elements and the curvature of the contour. The results show that the dynamics of contour integration are good when the contrast of the individual elements is modulated in time (10‐30 Hz), but are poor when contour linking per se is temporally modulated (1‐12 Hz). The dynamics of contour linking is not dependent on the absolute contrast of the linking elements, so long as they are visible, but does vary with the curvature of the contour. For straight contours, temporal resolution is around 6‐12 Hz but falls to around 1‐2 Hz for curved contours. © 2001 Elsevier Science Ltd. All rights reserved.
Influence of ethylene oxide gas treatment on the in vitro degradation behavior of dermal sheep collagen (1995)

The influence of ethylene oxide gas treatment on the in vitro degradation behavior of noncrosslinked, glutaraldehyde crosslinked or hexamethylene diisocyanate crosslinked dermal sheep collagen (DSC) using bacterial collagenase is described. The results obtained were compared with the degradation behavior of either nonsterilized or gamma-sterilized DSC. Upon ethylene oxide sterilization, reaction of ethylene oxide with the free amine groups of DSC occurred, which resulted in a decreased helix stability, as indicated by a lowering of the shrinkage temperature of all three types of DSC. Except for the low strain modulus the mechanical properties of the ethylene oxide sterilized materials were not significantly altered. gamma-Sterilization induced chain scission in all three types of DSC, resulting in a decrease of both the tensile strength and the high strain modulus of noncrosslinked and crosslinked DSC. When exposed to a solution of bacterial collagenase, ethylene oxide sterilized materials had a lower rate of degradation compared with nonsterilized DSC. This has been explained by a reduced adsorption of the collagenase onto the collagen matrix as a result of the introduction of pendant N-2-hydroxy ethyl groups.
Charakterisierung der Tropfenverteilung und ‐geschwindigkeit von Dispersionen mit Fasersensoren (2006)

Abstract Vorgestellt wird ein optisches Messverfahren, das für die Charakterisierung von Dispersionen, insbesondere von Suspensionen geeignet ist. Mit einem Sensor aus optischen Fasern wird das von flüssigen Tropfen reflektierte Licht detektiert. Durch die Analyse der Lichtsignale können die Geschwindigkeit, die Tropfengröße sowie die Position der Tropfen bestimmt werden.
A reconsideration of university gap funds for promoting biomedical entrepreneurship (2022)

Abstract The last 50 years have seen an increasing dependence on academic institutions to develop and commercialize new biomedical innovations, a responsibility for which many universities are ill-equipped. To address this need, we created LEAP, an asset development and gap fund program at Washington University in St. Louis (WUSTL). Beyond awarding funds to promising projects, this program aimed to promote a culture of academic entrepreneurship, and thus improve WUSTL technology transfer, by providing university inventors with individualized consulting and industry expert feedback. The purpose of this work is to document the structure of the LEAP program and evaluate its impact on the WUSTL entrepreneurial ecosystem. Our analysis utilizes program data, participant surveys, and WUSTL technology transfer office records to demonstrate that LEAP consistently attracted new investigators and that the training provided by the program was both impactful and highly valued by participants. We also show that an increase in annual WUSTL start-up formation during the years after LEAP was established and implicate the program in this increase. Taken together, our results illustrate that programs like LEAP could serve as a model for other institutions that seek to support academic entrepreneurship initiatives.
Self-crosslinkable poly(urethane urea)-reinforced silica aerogels (2015)

One-pot aerogel synthesis with sol–gel and reinforcement reactions in tandem. Reinforcing polymer molecules link silica networks at multiple (≥3) sites.
Abstract 14354: Cardiorenal Actions of the Designer Natriuretic Peptide, Cenderitide, in a Severe Heart Failure Model Treated With Furosemide Bolus (2011)

BACKGROUND: Diuretic therapy can provide symptomatic improvement in congestive heart failure (HF) although diuretic resistance and renal dysfunction can occur. Cenderitide (or CD-NP, “CD”), now in ...
High Shear in Situ Exfoliation of 2D Gallium Oxide Sheets from Centrifugally Derived Thin Films of Liquid Gallium (2021)

Herein, we have explored the use of a microfluidics platform for the exfoliation and oxidation of liquid gallium into ultrathin sheets of gallium oxide under continuous flow condition. The novel method developed here takes advantage of the high mass transfer in liquids and has the potential for creating high yielding thin sheets of oxidised gallium with insulating properties as well as acts as an active catalyst in hydrogen evolution reactions. This highlights the potential utility of the sheets as an alternative to the expensive and scarce noble metal based electrocatalysts
Electroluminescence from Ge1−ySny diodes with degenerate pn junctions (2015)

The light emission properties of GeSn pn diodes were investigated as a function of alloy composition and doping levels. Very sharp interfaces between contiguous ultra-highly doped p- and n-layers were obtained using in situ doping with B2H6 and P(SiH3)3 in a chemical vapor deposition environment, yielding nearly ideal model systems for systematic studies. Changes in the doping levels and layer Sn concentrations are shown to greatly affect the electroluminescence spectra. This sensitivity should make it possible to optimize the emission efficiency for these structures in the interesting quasi-direct regime, for which direct gap luminescence is observed due to the proximity of the conduction band quasi-Fermi level to the minimum of the conduction band at the center of the Brillouin zone. Such structures represent the basic building block of Ge-based electrically pumped lasers.
Performance control for interconnection of identical systems: Application to PLL network design (2014)

Summary In this paper, the problem of the control law design for interconnected identical systems ensuring the global stability and the global performance properties is under consideration. Inspired by the decentralized control law design methodology using the dissipativity input–output approach, the problem is reduced to the problem of satisfying two conditions: (i) the condition on the interconnection and (ii) the condition on the local subsystem dynamics. Both problems are efficiently solved applying a (quasi‐) convex LMI optimization and standard H ∞ synthesis. The proposed design methodology is applied to the control law design of a synchronous PLL network. Copyright © 2014 John Wiley &amp; Sons, Ltd.
Multilocus Sequence Typing of Historical Burkholderia pseudomallei Isolates Collected in Southeast Asia from 1964 to 1967 Provides Insight into the Epidemiology of Melioidosis (2006)

A collection of 207 historically relevant Burkholderia pseudomallei isolates was analyzed by multilocus sequence typing (MLST). The strain collection contains environmental isolates obtained from a geographical distribution survey of B. pseudomallei isolates in Thailand (1964 to 1967), as well as stock cultures and colony variants from the U.S. Army Medical Research Unit (Malaysia), the Walter Reed Army Institute for Research, and the Pasteur Institute (Vietnam). The 207 isolates of the collection were resolved into 80 sequence types (STs); 56 of these were novel. eBURST diagrams predict that the historical-collection STs segregate into three complexes when analyzed separately. When added to the 760 isolates and 365 STs of the B. pseudomallei MLST database, the historical-collection STs cluster significantly within the main complex of the eBURST diagram in an ancestral pattern and alter the B. pseudomallei "population snapshot." Differences in colony morphology among reference isolates were found not to affect the STs assigned, which were consistent with the original isolates. Australian ST84 is likely characteristic of B. pseudomallei isolates of Southeast Asia rather than Australia, since multiple environmental isolates from Thailand and Malaysia share this ST with the single Australian clinical isolate in the MLST database. Phylogenetic evidence is also provided suggesting that Australian isolates may not be distinct from those of Thailand, since ST60 is common to environmental isolates from both countries. MLST and eBURST are useful tools for the study of population biology and epidemiology, since they provide methods to elucidate new genetic relationships among bacterial isolates.
Blackbox Security of Biometrics (Invited Paper) (2010)

We analyze the security of biometric template protection methods that involve trusted hardware. The methods are defined in the black box security model, i.e., we consider components that perform operations on the biometric data they contain and only the input-output behaviour of these components is analyzed. The functionality that is implemented by these black boxes is assumed to be known, but as opposed to the white-box model no intermediate values can be observed. We illustrate our approach and demonstrate that additional countermeasures may be needed to protect the stored biometric data.
Supplementary Figure 3 from Identification of Existing Drugs That Effectively Target <i>NTRK1</i> and <i>ROS1</i> Rearrangements in Lung Cancer (2023)

&lt;p&gt;Inhibition of Ba/F3 CD74-ROS1 proliferation&lt;/p&gt;
High-Efficiency BODIPY-Based Organic Photovoltaics (2014)

A benzannulated boron dipyrromethene (BODIPY, bDIP) molecule exhibiting strong absorption at 640 nm was synthesized. The organic dye was used in an organic solar cell as the electron donor with C60 as the acceptor. The BODIPY dye demonstrated the best performance in lamellar architecture (indium tin oxide (ITO)/bDIP/C60/bathocuproine/Al), giving power conversion efficiency up to 4.5% with short-circuit current (JSC) of 8.7 mA/cm(2) and an open-circuit voltage (VOC) of 0.81 V. Neutron reflectivity experiments were performed on the bilayer film to investigate the thickness dependence of JSC. A 13 nm mixed layer was found to be present at the donor/acceptor interface in the bilayer device, formed when the C60 was deposited onto a room temperature bDIP film. Planar-mixed heterojunction devices were fabricated to understand the extent of spontaneous mixing between the donor and acceptor materials. The native mixed region in the bilayer device was shown to most resemble 1:3 bDIP:C60 layer in the structure: (ITO/bDIP/bDIP:C60 blend/C60/bathocuproine/Al).