1195 IN VITRO RECONSTITUTION OF HUMAN KIDNEY STRUCTURES FOR RENAL FAILURE (2011)

You have accessJournal of UrologyPediatrics: Reconstructive Surgery1 Apr 20111195 IN VITRO RECONSTITUTION OF HUMAN KIDNEY STRUCTURES FOR RENAL FAILURE Nadia Guimaraes-Souza, Tamer Aboushwareb, Kathryn Stern, Anthony Atala, and James Yoo Nadia Guimaraes-SouzaNadia Guimaraes-Souza Winston Salem, NC More articles by this author , Tamer AboushwarebTamer Aboushwareb Winston Salem, NC More articles by this author , Kathryn SternKathryn Stern Winston Salem, NC More articles by this author , Anthony AtalaAnthony Atala Winston Salem, NC More articles by this author , and James YooJames Yoo Winston Salem, NC More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.828AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES End stage renal disease is currently being treated effectively by transplantation. However, increasing demand and donor shortage make this treatment challenging. Recent advances in cell-based therapies have provided potential opportunities to alleviate the current challenges of donor shortage. We previously have demonstrated that single renal cells expanded in culture are able to form renal structures when implanted in vivo. However, the levels of structure formation could not be adequately controlled. In this study, we investigated whether human kidney structures could be pre-formed in vitro for subsequent implantation in vivo to maximize tissue forming efficiency. METHODS Primary human renal cells were isolated from unused donor kidneys using enzymatic digestion methods. Renal cells were grown, expanded and characterized using cell specific antibodies. To form kidney structures, single renal cells were placed in a three-dimensional culture system, consisting of neutralized type I collagen. The three-dimensional matrix with cells was solidified and cultivated over a period of 10 days. Histomorphological and ultrastructural analyses were performed using cell specific markers that identify proximal and distal tubules and collecting ducts. RESULTS Human primary renal cells were effectively isolated and expanded in culture. The cells retained their phenotypic and functional characteristics at multiple culture stages. Single renal cells placed in a three-dimensional culture environment began to proliferate and form structures that resemble renal tubules. Histologically, these structures showed phenotypic resemblance to native kidney structures. The reconstituted tubules stained positively for proximal and distal tubular markers. E-cadherin and N-cadherin staining confirmed polarization of the cells present in the tubules. CONCLUSIONS These findings show that single human renal cells grown in a three-dimensional culture system are able to generate kidney structures. The cells constituting these structures maintained the expression of renal cell specific markers. This system may ultimately be developed into an efficient cell-based therapy for patients with end stage renal disease. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e479 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Nadia Guimaraes-Souza Winston Salem, NC More articles by this author Tamer Aboushwareb Winston Salem, NC More articles by this author Kathryn Stern Winston Salem, NC More articles by this author Anthony Atala Winston Salem, NC More articles by this author James Yoo Winston Salem, NC More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...