gebhart/patrepeval huggingface dataset:
Growth control in simian virus 40-transformed rat cells: temperature-independent expression of the transformed phenotype in tsA transformants derived by agar selection (1978)

Fisher rat fibroblasts (FR 3T3), transformed with the tsA30 mutant of simian virus 40 and selected by colony formation in soft agar, maintained the transformed phenotype at high temperature, whereas most transformants isolated from foci were found to undergo a phenotypic reversion toward the normal state in their saturation density, ability to grow in soft agar, and rate of 2-deoxyglucose transport. The temperature-independent phenotype observed in agar-selected transformants was not due to a reversion of the viral mutation. These results, similar to those previously obtained with polyoma virus tsa mutants, further suggest that two distinct mechanisms may operate in both cases for maintaining the transformed phenotype. Immunofluorescence studies suggested a different regulation of T antigen synthesis in these two classes of transformants.
Design of Large Footings for One-Way Shear (2011)

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Development of Plus Ultrasonic Doppler Method for Flow Rate Measurements of Power Plant: Multiline Flow Rate Measurement for Non Developed Flow (2002)

Flow metering system is being developed using plus ultrasonic Doppler method. The principle is an integration of instantaneous velocity profile over a pipe diameter so that it is expected to be able to eliminate installation problems such as entry length as well as to follow transient flow rate precisely. Flow metering principle by plus ultrasonic Doppler method in a circular pipe depends on the alignment of measuring lines. In this paper, influence of number of measuring lines on the flow rate measurements for power plant have been investigated for non developed flows in a vertical pipe.
Onchidin B:  A New Cyclodepsipeptide from the Mollusc Onchidium sp. (1996)

Onchidin B (4) is a cyclic depsipeptide isolated from the pulmonate mollusc Onchidium sp. Its structure was determined by extensive 2D-NMR, FABMS, tandem FAB MS/MS, selective hydrolysis, and synthesis. It contains four α-amino acids [two units of N-methyl valine (MeVal), two units of proline (Pro)], four α-hydroxy acids [two 2-hydroxyisovaleric acids (Hiv), two 2-hydroxy-3-methylpentanoic acid moieties (Hmp)] and two units of the new β-hydroxy acid: 3-hydroxy-2-methyloct-7-ynoic acid (Hymo)]. Selective hydrolysis and direct comparison by chiral GC-MS with authentic samples of the α-amino and α-hydroxy acids allowed us the assignment of the entire absolute stereochemistry of onchidin B. In this way, the α-hydroxy acids were found to be (S)-Hiv and (S,S)-Hmp, and the α-amino acids (R)-proline, (S)-proline, and (R)-MeVal. In order to establish the absolute configuration of the new β-hydroxy acid, Hymo, its four possible stereoisomers were stereoselectively synthesized using chiral N-propionyl oxazolidinones and hex-5-ynal as starting material. Comparison by HPLC-MS of the synthetic samples with the natural Hymo (all derivatized as esters of (−)-(R)-α-methoxy-α-(9-anthryl)acetic acid), affirmed its absolute stereochemistry as (2R,3R). Thus, onchidin B (4) is cyclo [(R)-MeVal-(R,R)-Hymo-(S)-Pro-(S,S)-Hmp-(S)-Hiv-(R)-MeVal-(R,R)-Hymo-(R)-Pro-(S,S)-Hmp-(S)-Hiv]. It is formed by a head-to-tail linkage of two halves, each one built by five units and identical sequence. The lack of symmetry of onchidin B is thus due to the presence of one (S)-Pro unit in one half and one (R)-Pro unit in the other. The structural similarity between onchidin B (4) and onchidin (2), both isolated from the same organism, and between the β-hydroxy acid Hymo (5) and the β-amino acid Amo (3) found in onchidin is noticed.
ISG-2: properties of the second International Simple Glass (2023)

Abstract Given the importance of glass materials to society, their durability when exposed to aqueous solutions is a critical area for research, particularly for vitrified radioactive wastes. This spurred an international team to fabricate a standardized composition based on waste immobilization glass called the International Simple Glass (ISG), which has been the subject of numerous experimental and computational studies focused on aqueous corrosion resistance. With the original batch of ISG nearly depleted, the international team designed and fabricated a standard glass material, ISG-2, where half the Ca in the original composition was replaced with Mg by mole. This paper presents information on both the ISG-2 composition and a new batch with the same nominal composition as the original ISG, designated ISG-1, including their homogeneity, their physical and thermal properties. The results of static alteration experiments are presented as well to provide a baseline for future aqueous corrosion performance investigations.
NCOG-06. N107C/CEC.3 (ALLIANCE FOR CLINICAL TRIALS IN ONCOLOGY/CANADIAN CANCER TRIALS GROUP): PHASE III TRIAL OF POST-OPERATIVE RADIOSURGERY COMPARED WITH WHOLE BRAIN RADIOTHERAPY FOR RESECTED METASTATIC BRAIN DISEASE: COGNITIVE FUNCTION OF LONG-TERM SURVIVORS (2017)

It has been our recent finding that, when substituted for whole brain radiotherapy (WBRT) as an adjuvant to resection of a brain metastasis, radiosurgery (SRS) results in improved cognitive outcomes and equivalent overall survival — this despite inferior long-term local control. A subgroup of patients will be long-term survivors following local treatment of their brain metastases. Here we report an unplanned analysis of the outcomes of patients surviving more than 12 months following surgery. Patients were randomized to either radiosurgery or WBRT after resection of a brain metastasis. The primary endpoints were neurocognitive progression and overall survival. In this report, cognitive deterioration is defined as a 1-SD drop in at least 1 cognitive test. This study is registered with ClinicalTrials.gov, number NCT01372774. Between July 2011 and December 2015, 194 patients were enrolled with a median follow-up of 11·1 months (maximum 49·0 months). Baseline characteristics were well-balanced between study arms. At 12 months, there were 47 patients alive in the SRS arm and 42 in the WBRT arm; at 24 months, there were 14 and 22 survivors in the SRS and WBRT arms, respectively. At 12 months the intracranial control was 72% in the WBRT arm and 34% in the SRS arm (p<0.0001). At 12 months, 60% of SRS patients tested had neurocognitive progression vs. 91% of WBRT-treated patients (p<0.02). At 24 months, 40% of SRS patients tested had neurocognitive progression vs. 75% of WBRT-treated patients (p=0.2). At 24 months, no radiosurgery patient had a significant decline in immediate recall (HVLT-R) vs. 50% of WBRT patients (p=0.015). Decline in cognitive function occurs early after treatment of brain metastases and remains relatively stable over time. Despite inferior intracranial control, the cognitive advantage of SRS is preserved in long-term survivors. SUPPORT: U10CA180821, U10CA180882, U10CA180868, UG1CA189823.
Heterologous Immune Responses to Influenza Vaccine in Kidney Transplant Recipients (2016)

Influenza vaccine is known to have suboptimal immunogenicity in transplant recipients. Despite this, influenza vaccine may have the added benefit of inducing a cross-reactive immune response to viral strains not found in the vaccine. This is termed "heterologous immunity" and has not been assessed previously in transplant patients. Pre- and postvaccination sera from kidney transplant recipients (n = 60) immunized with the 2012-2013 adjuvanted or nonadjuvanted influenza vaccine underwent testing by hemagglutination inhibition assay for strains not present in vaccine: A/New Caledonia/20/99 (H1N1), A/Texas/50/2012 (H3N2) and B/Brisbane/60/2008. The geometric mean titer of antibody to heterologous strains increased after vaccine (H1N1: 80.0 to 136.1, p < 0.001; H3N2: 23.3 to 77.3, p < 0.001; B: 13.3 to 19.5, p < 0.001). Seroconversion rates were 16.7%, 41.7%, and 13.3%, respectively. No differences in heterologous response were seen in the adjuvanted versus nonadjuvanted groups. Patients were more likely to seroconvert for a cross-reactive antigen if they seroconverted for the specific vaccine antigen. Seroconversion to heterologous A/H3N2, for example, was 84.0% for homologous H3N2 seroconverters versus 11.4% for nonseroconverters (p < 0.001). This study provides novel evidence that transplant recipients are able to mount significant cross-protective responses to influenza vaccine that may be an additional, previously unknown benefit of immunization.
Aβ Oligomer Elimination Restores Cognition in Transgenic Alzheimer’s Mice with Full-blown Pathology (2018)

Oligomers of the amyloid-β (Aβ) protein are suspected to be responsible for the development and progression of Alzheimer's disease. Thus, the development of compounds that are able to eliminate already formed toxic Aβ oligomers is very desirable. Here, we describe the in vivo efficacy of the compound RD2, which was developed to directly and specifically eliminate toxic Aβ oligomers. In a truly therapeutic, rather than a preventive study, oral treatment with RD2 was able to reverse cognitive deficits and significantly reduce Aβ pathology in old-aged transgenic Alzheimer's Disease mice with full-blown pathology and behavioral deficits. For the first time, we demonstrate the in vivo target engagement of RD2 by showing a significant reduction of Aβ oligomers in the brains of RD2-treated mice compared to placebo-treated mice. The correlation of Aβ elimination in vivo and the reversal of cognitive deficits in old-aged transgenic mice support the hypothesis that Aβ oligomers are relevant not only for disease development and progression, but also offer a promising target for the causal treatment of Alzheimer's disease.
Fibroblast cell lines from young adult mice of long-lived mutant strains are resistant to multiple forms of stress (2005)

Previous studies have shown that dermal fibroblast cell lines derived from young adult mice of the long-lived Snell dwarf mutant stock are resistant, in vitro, to the cytotoxic effects of H(2)O(2), cadmium, UV light, paraquat, and heat. We show here that similar resistance profiles are seen in fibroblast cells derived from a related mutant, the Ames dwarf mouse, and that cells from growth hormone receptor-null mice are resistant to H(2)O(2), paraquat, and UV but not to cadmium. Resistance to UV light, cadmium, and H(2)O(2) are similar in cells derived from 1-wk-old Snell dwarf or normal mice, and thus the resistance of cell lines derived from young adult donors reflects developmental processes, presumably hormone dependent, that take place in the first few months of life. The resistance of cells from Snell dwarf mice to these stresses does not reflect merely antioxidant defenses: dwarf-derived cells are also resistant to the DNA-alkylating agent methyl methanesulfonate. Furthermore, inhibitor studies show that fibroblast resistance to UV light is unaffected by the antioxidants ascorbic acid and N-acetyl-L-cysteine. These data suggest that postnatal exposure to altered levels of pituitary hormones leads to development of cellular resistance to oxidative and nonoxidative stressors, which are stable through many rounds of in vitro cell division and could contribute to the remarkable disease resistance of long-lived mutant mice.
Noninvasive quantification of left ventricular rotational deformation in normal humans using magnetic resonance imaging myocardial tagging. (1990)

It has been postulated that rotation of the left ventricular apex with respect to the base is a component of normal systolic function in humans, but it has been difficult to measure it noninvasively. Tagging is a new magnetic resonance imaging technique that labels specific areas of myocardium by selective radio-frequency excitation of narrow planes orthogonal to the imaging plane before acquiring an image. Tags appear as black lines and persist in myocardium for 400-500 msec and, if applied at end diastole, will move with the myocardium through systole. Tagging was used to noninvasively quantify left ventricular torsion and circumferential-longitudinal shear (shearCL) in humans. Eight normal volunteers, aged 24-38 years, were imaged in a 0.38-T iron-core resistive magnet. Five short-axis left ventricular images, positioned to encompass the entire left ventricle (LV), were obtained separately at end systole. Four equiangular radial tags had been applied at end diastole, intersecting the myocardium at eight locations. We calculated the difference in angular displacement of each epicardial and endocardial tag point (a tag point being where the tag crossed the epicardium or endocardium) at end systole from the systolic position of the corresponding tag point on the basal plane. This value was called the torsion angle. From this, shearCL, the angle inscribed on the epicardial or endocardial surface between the systolic tag position, the corresponding basal tag position, and its projection onto the slice of interest could be calculated at 32 points in the left ventricular wall.(ABSTRACT TRUNCATED AT 250 WORDS)
Texture processing of extruded YBa2Cu3O6+x wires by zone melting (1990)

Zone melting has been used to produce texture in extruded YBa2Cu3O6+x wires. By controlling the directional solidification of the molten zone, oriented structures are produced that show a large magnetic hysteresis at 77 K compared to unaligned extruded YBa2Cu3O6+x wires. A magnetization critical current density of 1.8 × 104 A/cm2 in an applied field of 20 kOe has been achieved.
Proteasome Activation is a Mechanism for Pyrazolone Small Molecules Displaying Therapeutic Potential in Amyotrophic Lateral Sclerosis (2014)

Amyotrophic lateral sclerosis (ALS) is a progressive and ultimately fatal neurodegenerative disease. Pyrazolone containing small molecules have shown significant disease attenuating efficacy in cellular and murine models of ALS. Pyrazolone based affinity probes were synthesized to identify high affinity binding partners and ascertain a potential biological mode of action. Probes were confirmed to be neuroprotective in PC12-SOD1(G93A) cells. PC12-SOD1(G93A) cell lysates were used for protein pull-down, affinity purification, and subsequent proteomic analysis using LC-MS/MS. Proteomics identified the 26S proteasome regulatory subunit 4 (PSMC1), 26S proteasome regulatory subunit 6B (PSMC4), and T-complex protein 1 (TCP-1) as putative protein targets. Coincubation with appropriate competitors confirmed the authenticity of the proteomics results. Activation of the proteasome by pyrazolones was demonstrated in the absence of exogenous proteasome inhibitor and by restoration of cellular protein degradation of a fluorogenic proteasome substrate in PC12-SOD1(G93A) cells. Importantly, supplementary studies indicated that these molecules do not induce a heat shock response. We propose that pyrazolones represent a rare class of molecules that enhance proteasomal activation in the absence of a heat shock response and may have therapeutic potential in ALS.
New strategies for ventricular tachycardia and ventricular fibrillation ablation (2015)

Patients with ventricular tachycardia (VT) and ventricular fibrillation (VF) and no reversible cause are difficult to treat. While implantable defibrillators prolong survival, many patients remain symptomatic due to device shocks and syncope. To address this, there have been recent advances in the catheter ablation of VT and VF. For example, non-invasive imaging has improved arrhythmia substrate characterisation, 3D catheter navigation tools have facilitated mapping of arrhythmia and substrate and ablation catheters have advanced in their ability to deliver effective lesions. However, the long-term success rates of ablation for VT and VF remain modest, with nearly half of treated patients developing recurrence within 2-3 years, and this drives the ongoing innovation in the field. This review focuses on the challenges particular to ablation of life-threatening ventricular arrhythmia, and the strategies that have been recently developed to improve procedural efficacy. Patient sub-groups that illustrate the use of new strategies are described.
Assessment of tissue oxygen saturation during a vascular occlusion test using near-infrared spectroscopy: role of the probe spacing and measurement site studied in healthy volunteers (2009)

To assess potential metabolic and microcirculatory alterations in critically ill patients, near-infrared spectroscopy (NIRS) has been used, in combination with a vascular occlusion test (VOT), for the noninvasive measurement of tissue oxygen saturation (StO2), oxygen consumption, and microvascular reperfusion and reactivity. However, the methodologies for assessing StO2 are very inconsistent in the literature and results vary from study to study. In this study we investigated the effects of the probe spacing and measurement site, using both a 15 mm and a 25 mm probe spacing on the thenar and the forearm, in healthy volunteers.
Direct detection and drug-resistance profiling of bacteremias using inertial microfluidics (2015)

Detection of bacteria in bloodstream infections and their antibiotic susceptibility patterns is critical to guide therapeutic decision-making for optimal patient care. Current culture-based assays are too slow (>48 h), leading to excessive up-front use of broad-spectrum antibiotics and/or incorrect antibiotic choices due to resistant bacteria, each with deleterious consequences for patient care and public health. To approach this problem, we describe a method to rapidly isolate bacteria from whole blood using inertial microfluidics and directly determine pathogen identity and antibiotic susceptibility with hybridization-based RNA detection. Using the principle of Dean flow fractionation, bacteria are separated from host blood cells in a label-free separation method with efficient recovery of even low abundance bacteria. Ribosomal RNA detection can then be applied for direct identification of low abundance pathogens (~100 per mL) from blood without culturing or enzymatic amplification. Messenger RNA detection of antibiotic-responsive transcripts after brief drug exposure permits rapid susceptibility determination from bacteria with minimal culturing (~105 per mL). This unique coupling of microfluidic cell separation with RNA-based molecular detection techniques represents significant progress towards faster diagnostics (~8 hours) to guide antibiotic therapy.
Residential Photovoltaics; An Investment Vehicle for Retiree's (2006)

Can a residential photovoltaic system provide all of the elements that would qualify it for consideration as a component of a retiree's investment portfolio? To address this question one must consider the maturity of the technology, investment amount, derived revenue stream and overall risk. These factors can then be compared to conventional investment vehicles. A retirement investment vehicle should be capable of providing an income stream, which keeps pace with inflation for an indefinite period. This paper will provide actual financial performance data from a 6 KW DC/5 KW AC residential photovoltaic system with the performance of conventional retirement investment vehicles. The methodology for performing the economic analysis along with tax implications for each investment vehicle is presented. This will allow individuals to determine if a residential photovoltaic system should be considered as investment vehicle for retirement planning
Adjuvant Imatinib in Patients with GIST Harboring Exon 9 KIT Mutations: Results from a Multi-institutional European Retrospective Study (2021)

In this retrospective series of patients with KIT exon 9-mutated GIST treated with adjuvant imatinib, a daily dose of 800 mg versus 400 mg did not show better results in terms of survival outcomes. Prospective evaluation of the more appropriate adjuvant treatment in this setting is warranted.
La Induced Passivation of High-kBulk and Interface Defects in Polycrystalline Silicon/TiN/HfLaSiO/SiO2Stacks (2011)

La incorporation into Hf-based gate dielectrics is a promising methodology for achieving low threshold voltage (Vth) metal/high-k n-channel metal–oxide–semiconductor field-effect transistors (nMOSFETs) with the gate-first process. To clarify the impact of the Hf/La ratio in high-k dielectrics on device performance, we investigated high-k bulk and interface traps of polycrystalline silicon (poly-Si)/TiN/HfLaSiO/SiO2 stacks with various Hf/La ratios. We found that La incorporation is effective for improving electron mobility; however, in a pure LaSiO device, the mobility is degraded. Our charge-pumping (CP) measurements revealed that both high-k bulk traps and near-interface traps (Nit) near the conduction band, which cause mobility degradation, can be effectively passivated by La incorporation. These results imply that an optimized La ratio will lead to superior nMOSFET performance, while an appropriate Vth can be tuned.
Modeling and Analysis of the Bendable Transformer (2015)

This paper presents a study of a bendable transformer for wearable electronics. Printed on a thin and bendable film, this transformer is bendable to wrap around body limbs such as the forearm. A model using a partial equivalent circuit theory has been developed to analyze the characteristic of an inductor and a bendable transformer. The mutual inductance and self-inductance for the bendable transformer over a range of bent curvatures have been calculated based on the model and compared favorably with measurements. Simulation and experimental results of applying the bendable inductor and transformer in dc-dc converters as a 5-V 500-mA power supply are included to confirm the usefulness of the transformer and the validity of the model.
Self-aggregated perfluoroalkylated hexa-peri-hexabenzocoronene fibers observed by cryo-SEM and fluorescence spectroscopy (2006)

The self-assembled architectures in solution of a new HBC derivative bearing perfluoroalkylated side chains were investigated by optical excitation and emission spectroscopy and correlated to cryo-SEM, a new technique in organic chemistry.
A Computationally Efficient Retina Detection and Enhancement Image Processing Pipeline for Smartphone-Captured Fundus Images (2018)

International audience
Study of Full-Length Porcine Endogenous Retrovirus Genomes with Envelope Gene Polymorphism in a Specific-Pathogen-Free Large White Swine Herd (2000)

Specific-pathogen-free (SPF) swine appear to be the most appropriate candidate for pig to human xenotransplantation. Still, the risk of endogenous retrovirus transmission represents a major obstacle, since two human-tropic porcine endogenous retroviruses (PERVs) had been characterized in vitro (P. Le Tissier, J. P. Stoye, Y. Takeuchi, C. Patience, and R. A. Weiss, Nature 389:681-682, 1997). Here we addressed the question of PERV distribution in a French Large White SPF pig herd in vivo. First, PCR screening for previously described PERV envelope genes envA, envB, and envC (D. E. Akiyoshi, M. Denaro, H. Zhu, J. L. Greenstein, P. Banerjee, and J. A. Fishman, J. Virol. 72:4503-4507, 1998; Le Tissier et al., op. cit.). demonstrated ubiquity of envA and envB sequences, whereas envC genes were absent in some animals. On this basis, selective out-breeding of pigs of remote origin might be a means to reduce proviral load in organ donors. Second, we investigated PERV genome carriage in envC negative swine. Eleven distinct full-length PERV transcripts were isolated. The sequence of the complete envelope open reading frame was determined. The deduced amino acid sequences revealed the existence of four clones with functional and five clones with defective PERV PK-15 A- and B-like envelope sequences. The occurrence of easily detectable levels of PERV variants in different pig tissues in vivo heightens the need to assess PERV transmission in xenotransplantation animal models.