gebhart/patrepeval huggingface dataset:
Eight cycles of BEACOPP escalated compared with 4 cycles of BEACOPP escalated followed by 4 cycles of BEACOPP baseline with or without radiotherapy in patients in advanced stage Hodgkin lymphoma (HL): Final analysis of the HD12 trial of the German Hodgkin Study Group (GHSG) (2009)

8544 Background: The GHSG HD9 trial had established BEACOPP escalated (BE) as new standard of care for advanced-stage HL patients. The successor study, HD12, evaluated a possible reduction in toxicity by comparing 8 cycles of BE with 4 cycles BE followed by 4 cycles BB. The second question in this trial related to the need of additional radiotherapy (RT) to initial bulk and residual disease. Methods: HL patients in stage IIB with large mediastinal mass and/or E-lesions or stage III/IV were randomised according to a 2x2-factorial design between: 8BE + RT, 8BE no RT, 4BE+4BB + RT, 4BE+4BB no RT. Primary endpoint of the trial was FFTF. Between 9/1999 and 1/2003, a total of 1,670 patients aged 16–65 were randomized. For this final analysis at a median follow up of 78 months, 99 patients were excluded for various reasons resulting in 1,571 eligible patients. Results: Patient characteristics in the 4 groups were comparable. Treatment-related toxicity of WHO grade III/IV was observed in 97% of patients. Most prominent differences between pooled chemotherapy arms were anemia (65% 8BE vs 51% 4BE+4BB) and thrombopenia (65% vs 51%). Treatment outcome: complete remission 92.4%; early progression 2.2%; progression/relapse 7.8% (6.6% and 8.5%). A total of 156 (9.9%) deaths (72 vs 84) have been observed (22 vs 32 acute or salvage treatment toxicity; 15 vs 24 HL; 22 vs 13 secondary neoplasia). Most treatment related deaths occurred in the &gt;60 years age group, the first 4 cycles and the IPS&gt; 3 RF groups. Secondary neoplasias were observed in 77 patients (4.9%). At 5 years, OS was 91%, FFTF 85.5% and progression free survival (PFS) 86.2% (Kaplan-Meier estimates). Estimates for the difference at 5 years are 1.8% for OS, 2.3% for FFTF and 2.7% for PFS favoring BE. However, there was no statistical difference between 8x BE and 4BE+4BB in all outcome parameters (p&gt;0.19, log rank test). There is also no significant difference between the RT or no-RT arms in this study. Conclusions: The adoption of 4BE+4BB as a new standard in the future GHSG studies will depend on a refined analysis of the total data set and will be presented. No significant financial relationships to disclose.
Adaptive optics allows STED-FCS measurements in the cytoplasm of living cells (2019)

Fluorescence correlation spectroscopy in combination with super-resolution stimulated emission depletion microscopy (STED-FCS) is a powerful tool to investigate molecular diffusion with sub-diffraction resolution. It has been of particular use for investigations of two dimensional systems like cell membranes, but has so far seen very limited applications to studies of three-dimensional diffusion. One reason for this is the extreme sensitivity of the axial (z) STED depletion pattern to optical aberrations. We present here an adaptive optics-based correction method that compensates for these aberrations and allows STED-FCS measurements in the cytoplasm of living cells.
Data from Small-Molecule ONC201/TIC10 Targets Chemotherapy-Resistant Colorectal Cancer Stem–like Cells in an Akt/Foxo3a/TRAIL–Dependent Manner (2023)

&lt;div&gt;Abstract&lt;p&gt;Self-renewing colorectal cancer stem/progenitor cells (CSC) contribute to tumor maintenance and resistance to therapy. Therapeutic targeting of CSCs could improve treatment response and prolong patient survival. ONC201/TIC10 is a first-in-class antitumor agent that induces TRAIL pathway–mediated cell death in cancer cells without observed toxicity. We have previously described that ONC201/TIC10 exposure leads to transcriptional induction of the TRAIL gene via transcription factor Foxo3a, which is activated by dual inactivation of Akt and ERK. The Akt and ERK pathways serve as important targets in CSCs. Foxo3a is a key mediator of Akt and ERK-mediated CSC regulation. We hypothesized that the potent antitumor effect of ONC201/TIC10 in colorectal cancer involves targeting CSCs and bulk tumor cells. ONC201/TIC10 depletes CD133&lt;sup&gt;+&lt;/sup&gt;, CD44&lt;sup&gt;+&lt;/sup&gt;, and Aldefluor&lt;sup&gt;+&lt;/sup&gt; cells &lt;i&gt;in vitro&lt;/i&gt; and &lt;i&gt;in vivo&lt;/i&gt;. TIC10 significantly inhibits colonosphere formation of unsorted and sorted 5-fluorouracil–resistant CSCs. ONC201/TIC10 significantly reduces CSC-initiated xenograft tumor growth in mice and prevents the passage of these tumors. ONC201/TIC10 treatment also decreased xenograft tumor initiation and was superior to 5-fluorouracil treatment. Thus, ONC201/TIC10 inhibits CSC self-renewal &lt;i&gt;in vitro&lt;/i&gt; and &lt;i&gt;in vivo&lt;/i&gt;. ONC201/TIC10 inhibits Akt and ERK, consequently activating Foxo3a and significantly induces cell surface TRAIL and DR5 expression in both CSCs and non-CSCs. ONC201/TIC10-mediated anti-CSC effect is significantly blocked by the TRAIL sequestering antibody RIK-2. Overexpression of Akt, DR5 knockdown, and Foxo3a knockdown rescues ONC201/TIC10-mediated depletion of CD44&lt;sup&gt;+&lt;/sup&gt; cells and colonosphere inhibition. In conclusion, ONC201/TIC10 is a promising agent for colorectal cancer therapy that targets both non-CSCs and CSCs in an Akt–Foxo3a–TRAIL–dependent manner. &lt;i&gt;Cancer Res; 75(7); 1423–32. ©2015 AACR&lt;/i&gt;.&lt;/p&gt;&lt;/div&gt;
Combustion Chamber Deposit Flaking and Startability Problems in Three Different Engines (2003)

&lt;div class="htmlview paragraph"&gt;A field problem associated with flakes of combustion chamber deposits getting trapped on the exhaust valve seat and causing starting problems has appeared recently.&lt;/div&gt; &lt;div class="htmlview paragraph"&gt;Four fuels have been tested in three different car models using a deposit flaking road test procedure. For each piston top, flaking can be characterised using T1 and T2, the mean deposit thickness on the piston crown before and after flaking respectively. A new measure of deposit flaking, ΔT, the mean of (T1-T2) averaged over all cylinders has been introduced and its variance established for the standard test using one of the models. ΔT quantifies the actual amount of deposits that have flaked and is likely to be a more relevant indicator of flaking for startability problems than R&lt;sub&gt;w&lt;/sub&gt;, the mean of the ratio of T2 to T1, used in previous work. Deposit flaking is directly related to an increase in valve leakage rates and startability problems. For individual cylinders, there is a sharp transition to a high leakage rate and failure to start if (T1-T2) exceeds about 55 microns or if (T2/T1) decreases below 0.5. A simple probabilistic model has been developed to assess the impact of flaking. Comparison of the results of this model with experimental results suggests that there is a very high probability of loose flakes getting trapped on the valve seat and the probability of the consequent failure to start increases very sharply above a threshold amount of flakes. Hence the best approach to ensure against failure to start is to make sure that deposit flaking is minimised.&lt;/div&gt;
MMSE Estimation of Sparse Lévy Processes (2012)

We investigate a stochastic signal-processing framework for signals with sparse derivatives, where the samples of a Lévy process are corrupted by noise. The proposed signal model covers the well-known Brownian motion and piecewise-constant Poisson process; moreover, the Lévy family also contains other interesting members exhibiting heavy-tail statistics that fulfill the requirements of compressibility. We characterize the maximum-a-posteriori probability (MAP) and minimum mean-square error (MMSE) estimators for such signals. Interestingly, some of the MAP estimators for the Lévy model coincide with popular signal-denoising algorithms (e.g., total-variation (TV) regularization). We propose a novel non-iterative implementation of the MMSE estimator based on the belief-propagation (BP) algorithm performed in the Fourier domain. Our algorithm takes advantage of the fact that the joint statistics of general Lévy processes are much easier to describe by their characteristic function, as the probability densities do not always admit closed-form expressions. We then use our new estimator as a benchmark to compare the performance of existing algorithms for the optimal recovery of gradient-sparse signals.
Rate-Splitting Multiple Access for Intelligent Reflecting Surface Aided Multi-User Communications (2021)

Intelligent reflecting surface (IRS) has recently emerged as a promising technology for 6 G wireless systems, due to its capability to reconfigure the wireless propagation environment. In this paper, we investigate a Rate-Splitting Multiple Access (RSMA) for IRS-assisted downlink system, where the base station (BS) communicates with single-antenna users with the help of an IRS. RSMA relies on rate-splitting (RS) at the BS and successive interference cancellation (SIC) at the users and provides a generalized multiple access framework. We derive a new architecture called IRS-RS that leverages the interplay between RS and IRS. For performance analysis, we utilize an on-off control technique to control the passive beamforming vector of the IRS-RS and derive the closed-form expressions for outage probability of cell-edge users and near users. Moreover, we also analyze the outage behavior of cell-edge users for a sufficiently large number of reflecting elements. Additionally, we also analyze the outage performance of cooperative RS based decode-and-forward (DF)-assisted framework called DF-RS. Through simulation results, it is shown that the proposed framework outperforms the corresponding DF-RS, RS without IRS and IRS-assisted conventional non-orthogonal multiple access (NOMA) schemes. Furthermore, the impact of various system's parameters such as the number of IRS reflecting elements and the number of users on the system performance is revealed.
Capillary extraction by detecting polarity in circular profiles (2016)

Quantitative characterisation of blood vessels from images (e.g. morphometric analysis) is important to a variety of biomedical problems such as disease diagnosis and staging or assessment of angiogenesis. However, the accuracy of such characterisation depends heavily on the outcome of image preprocessing algorithms. Therefore, more efficient algorithms for vessel image segmentation or extraction have emerged within the past few years. Nevertheless, such methods may perform poorly or fail entirely for images with large noise, even after a careful tuning of parameters. Moreover, none of these methods intentionally considers the removal of structural noise (such as spots that obscure and/or are brighter than vessels). To address these issues, the authors propose a novel thresholding algorithm for capillary images by detecting the polarity in the circular profiles (PCPs) of image pixels. This can robustly distinguish tube‐like objects from both cloud‐like contaminations and structural noise. Extensive simulation studies based on multiple evaluation criteria suggest that the PCP algorithm typically has a superior performance over other representative approaches. Finally, they also demonstrate the satisfactory performance of the PCP method on real image data.
Silver atoms and small silver clusters stabilized in zeolite Y: optical spectroscopy (1983)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSilver atoms and small silver clusters stabilized in zeolite Y: optical spectroscopyGeoffrey A. Ozin and Francois HuguesCite this: J. Phys. Chem. 1983, 87, 1, 94–97Publication Date (Print):January 1, 1983Publication History Published online1 May 2002Published inissue 1 January 1983https://pubs.acs.org/doi/10.1021/j100224a022https://doi.org/10.1021/j100224a022research-articleACS PublicationsRequest reuse permissionsArticle Views559Altmetric-Citations95LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Abstract 077: In Vivo Evidence of AT1a Receptor-Mediated Uptake of Angiotensin II by the Proximal Tubule Visualized by Intravital Multiphoton Imaging (2015)

The development of all forms of angiotensin II (ANG II)-dependent hypertension is associated with higher levels of intrarenal ANG II, which are greater than can be explained on the basis of circulating ANG II and suppressed cortical renin expression. In the present study, we used intravital multiphoton imaging to test the hypothesis that AT1 (AT1a) receptor-mediated uptake of ANG II by the proximal tubules of the kidney plays a major role in the underlying mechanisms. Adult male Munich-Wistar rats were anesthetized with Inactin and continuously infused with a pressor dose of Alexa 488-conjugated ANG II (50 ng/min, i.v.) for 2 hr. Time-dependent proximal tubular uptake responses of Alexa 488-ANG II were studied with mean arterial blood pressure maintained at ~150 mmHg throughout the experiment. After 30 min infusion, very low levels of Alexa 488-ANG II were visualized within proximal tubules and cortical collecting ducts (CCDs) (p&lt;0.05). However, high levels of Alexa 488-ANG II were accumulated in the lumen of CCDs, but not that of proximal tubules. After 1 hr infusion, moderate levels of Alexa 488-ANG II were visualized in the proximal tubules, but not in the glomeruli and CCDs. The most striking uptake responses were visualized in all segments of proximal tubules after 2 hr infusion. Internalized Alexa 488-ANG II was predominantly localized to the basolateral side, where it was colocalized with tetramethyl rhodamine methyl ester (TMRM), a mitochondrial membrane potential-dependent dye. TMRM is a lipophilic cationic dye that is primarily accumulated in the mitochondria of proximal tubules. Some internalized Alexa 488-ANG II was visualized around and over the nuclei. Furthermore, the uptake of Alexa 488-ANG II by proximal tubules was significantly attenuated in caveolin 1-knockout mice (p&lt;0.01), and blocked in AT1a receptor-knockout mice (p&lt;0.01). Our results provide strong intravital multiphoton microscopic evidence that circulating ANG II is primarily taken up by the proximal tubules of the kidney via an AT1a receptor-mediated mechanism, and that internalized ANG II may be transported to the mitochondria and the nucleus, where it may alter mitochondrial and nuclear function in the proximal tubules of the kidney.
Pro‐insecticidal approach towards increasing in planta activity (2016)

Adrenergic active compounds, such as clonidine analogs, show potential as insecticides; however, a designed prodrug approach did not overcome the lack of translation in this case. Studies confirmed that the synthesized prodrug analog metabolized in planta to the proposed vacuole-trapped compound. One possible explanation for the failure of this approach is that the rate of metabolism and vacuole trapping is faster than translaminar flow, and therefore the released pesticide is not biologically available to the target organism. © 2016 Society of Chemical Industry.
Apparent steady-state kinetic constants of wild-type and mutant POx with D-glucose as electron donor (100 mM) and DCPIP (varied from 0.015–1.2 mM) as electron acceptor. (2015)

&lt;p&gt;Apparent steady-state kinetic constants of wild-type and mutant POx with D-glucose as electron donor (100 mM) and DCPIP (varied from 0.015–1.2 mM) as electron acceptor.&lt;/p&gt;
A porous Ge/Si interface layer for defect-free III-V multi-junction solar cells on silicon (2019)

III-V solar cell cost reduction and direct III-V/Si integration can both be realized by depositing a thin layer of high-quality Ge on relatively low-cost Si substrates. However, direct epitaxial growth of Ge on Si substrates is difficult due to the 4% lattice mismatch between the film and the substrate. Threading dislocations (TDs) introduced within the Ge layer have a detrimental effect on device performances. The goal of this research is to address the perennial need to minimize the defect density of Ge epilayers grown on a Si substrate. We seek to accommodate the effects of the lattice mismatch by introducing a porous Si interface layer to intercept dislocations and prevent them from reaching the active layers of the device. The porous Si layer is formed through dislocation-selective electrochemical deep etching and thermal annealing. The porous layer created beneath the top Ge layer can both act as dislocation traps and as a soft compliant substrate, which displays high flexibility. Transmission electron microscopy (TEM) analysis of the Ge/porous Si interface shows that the lattice mismatch strain of the Ge films was almost relaxed. The surface roughness of this modified Ge/Si substrate has been reduced using chemical mechanical polishing (CMP) process to fulfil the requirements for epitaxy of III-V alloys. Finally, we present simulation results exploring the effect of threading dislocations on device performance.
Light factorization for mixed-frequency shadows in augmented reality (2011)

Integrating animated virtual objects with their surroundings for high-quality augmented reality requires both geometric and radio-metric consistency. We focus on the latter of these problems and present an approach that captures and factorizes external lighting in a manner that allows for realistic relighting of both animated and static virtual objects. Our factorization facilitates a combination of hard and soft shadows, with high-performance, in a manner that is consistent with the surrounding scene lighting.
Measurement and modeling of the multi-wavelength optical properties ofuncoated flame-generated soot (2018)

Abstract. Optical properties of flame-generated black carbon (BC) containing soot particles were quantified at multiple wavelengths for particles produced using two different flames, a methane diffusion flame and an ethylene premixed flame. Measurements were made for: (i) nascent soot particles, (ii) thermally denuded nascent particles, and (iii) particles that were coated then thermally denuded, leading to collapse of the initially lacy, fractal-like morphology. The measured mass absorption coefficients (MAC) depended on soot maturity and generation, but were similar between flames for similar conditions. For mature soot, here corresponding to particles with volume-equivalent diameters &gt; ~ 160 nm, the MAC and absorption Angstrom exponent values were independent of particle collapse while the single scatter albedo increased. The MAC values for these larger particles were also size-independent. Effective, theory-specific complex refractive index (RI) values are derived from the observations with two widely-used methods: Lorenz-Mie theory and the Rayleigh-Debye-Gans (RDG) approximation. Mie theory systematically under-predicts the observed absorption cross-sections at all wavelengths for larger particles (with x &gt; 0.9) independent of the complex RI used, while RDG provides good agreement. Importantly, this implies that the use of Mie theory within air quality and climate models, as is common, likely leads to under-predictions in the absorption by BC, with the extent of under-prediction depending on the assumed BC size distribution and complex RI used. We suggest that it is more appropriate to assume a constant, size-independent (but wavelength-specific) MAC to represent absorption by uncoated BC particles within models.
Susceptibility of Cell Populations to Transduction by Retroviral Vectors (2004)

Retroviral transduction efficiency is related to the multiplicity of infection and the physiological state of the target cells. It is generally not known what proportion of a cell population is susceptible to transduction. We used coinfection with two retroviral vectors containing the marker genes for green fluorescent protein and the truncated human nerve growth factor receptor. In the CD34+ cell line TF-1 or human primary CD34+ hematopoietic progenitor cells, it was found that cells transduced with one vector had a better than random chance of transduction by the other vector. A probability model was developed to estimate target cell susceptibility; susceptibility was calculated as the product of the proportions of transgene-positive cells divided by the proportion of double-positive cells. By using this relationship, it was found that susceptibility was related to the target cell type and culture conditions but not the retroviral titer or the retroviral packaging envelope protein used in this study. Cotransduction with two vectors is a relatively simple procedure that provides a means to assess the maximum transduction level possible in a given cell population.
Nlrx1-Regulated Defense and Metabolic Responses to Aspergillus fumigatus Are Morphotype and Cell Type Specific (2021)

The Nlr family member X1 (Nlrx1) is an immuno-metabolic hub involved in mediating effective responses to virus, bacteria, fungi, cancer, and auto-immune diseases. We have previously shown that Nlrx1 is a critical regulator of immune signaling and mortality in several models of pulmonary fungal infection using the clinically relevant fungus <i>Aspergillus fumigatus</i>. In the absence of Nlrx1, hosts produce an enhanced Th2 response primarily by CD103+ dendritic cell populations resulting in enhanced mortality <i>via</i> immunopathogenesis as well as enhanced fungal burden. Here, we present our subsequent efforts showcasing loss of Nlrx1 resulting in a decreased ability of host cells to process <i>A. fumigatus</i> conidia in a cell-type-specific manner by BEAS-2B airway epithelial cells, alveolar macrophages, bone marrow-derived macrophages, but not bone marrow-derived neutrophils. Furthermore, loss of Nlrx1 results in a diminished ability to generate superoxide and/or generic reactive oxygen species during specific responses to fungal PAMPs, conidia, and hyphae. Analysis of glycolysis and mitochondrial function suggests that Nlrx1 is needed to appropriately shut down glycolysis in response to <i>A. fumigatus</i> conidia and increase glycolysis in response to hyphae in BEAS-2B cells. Blocking glycolysis and pentose phosphate pathway (PPP) <i>via</i> 2-DG and NADPH production through glucose-6-phosphate dehydrogenase inhibitor resulted in significantly diminished conidial processing in wild-type BEAS-2B cells to the levels of Nlrx1-deficient BEAS-2B cells. Our findings suggest a need for airway epithelial cells to generate NADPH for reactive oxygen species production in response to conidia <i>via</i> PPP. In context to fungal pulmonary infections, our results show that Nlrx1 plays significant roles in host defense <i>via</i> PPP modulation of several aspects of metabolism, particularly glycolysis, to facilitate conidia processing in addition to its critical role in regulating immune signaling.