gebhart/patrepeval huggingface dataset:
Creatine supplementation???Part II: in vivo magnetic resonance spectroscopy (1999)

Noninvasive observation of Cr and PCr after Cr supplementation shows an increase in a muscle specific manner. Higher preexercise levels of PCr/ATP at rest compensate for significantly slower recovery rates of PCr/ATP after Cr supplementation.
Evaluation of high thermal stability cyclopentadienyl Hf precursors with H2O as a co-reactant for advanced gate logic applications (2011)

For the purpose of extending the upper temperature limit of metallorganic atomic layer deposition, mixed ligand precursors containing cyclopentadienyl (Cp, C5H5) ligands have been shown to exhibitsuperior thermal stability compared to the widely adopted tetrakis(ethylmethylamino)hafnium (TEMAH) precursor while also possessing adequate vapor pressure characteristics for use in atomic layer deposition (ALD) processing. In order to prevent the deleterious oxidation of the underlying Si from O3 the use of a milder oxidant such as H2O is preferred. Accordingly in this study, we investigated ALD using the liquid precursors CpHf(NMe2)3 and (CpMe)2Hf(OMe)Me in the temperature range 305 – 410 °C with H2O as a co-reactant and compared the film growth and electrical properties with films deposited using a conventional TEMAH/H2O process at 305 °C as well as the same process with an optimized annealing scheme. The CpHf(NMe2)3/H2O process was observed toexhibit a growth-per-cycle (GPC) in the range 0.23 – 0.36 Å/cycle which is roughly half that of the TEMAH/H2O process (∼0.6 Å/cycle). In the 340 – 375 °C range this process is comparable toTEMAH/H2O in terms of equivalent oxide thickness but with slightly higher leakage. Even thoughthis process allows for higher processing temperatures it is not able to scale to the level of theTEMAH/H2O process using an optimized annealing scheme. For (CpMe)2Hf(OMe)Me withH2O,in the process space investigated in this study this process was unable to afford a viable growth rate (&amp;lt;0.03 Å/cycle) for device testing although a GPC of 0.33 Å/cycle was obtained using O3.
A low-IF RX two-point ΣΔ-modulation TX CMOS single-chip Bluetooth solution (2001)

A new low-cost concept for a system-on-chip Bluetooth solution is proposed in this paper. The single chip includes all necessary baseband and RF parts to achieve full Bluetooth functionality and is implemented in a standard 0.25-/spl mu/m CMOS technology. The two-point modulation /spl Sigma//spl Delta/ fractional N phase-locked loop achieves a phase noise of -124 dBc/Hz at 3-MHz offset. The sensitivity of the embedded low-IF receiver is measured to be -82 dBm at a bit error rate of 0.1%. The power supply voltages for the digital and analog parts are internally regulated to 2.65 V. The maximum current consumption of the analog part is 60 mA.
Table S2 from First-in-Class ERK1/2 Inhibitor Ulixertinib (BVD-523) in Patients with MAPK Mutant Advanced Solid Tumors: Results of a Phase I Dose-Escalation and Expansion Study (2023)

&lt;p&gt;Dose levels enrolled in dose escalation with number and description of dose limiting toxicities at each dose level.&lt;/p&gt;
Development of aLAC4 promoter-based gratuitous induction system inKluyveromyces lactis (2000)

A gratuitous induction system based on the strong, indigenous LAC4 promoter was developed for Kluyveromyces lactis. To prevent consumption of the inducer galactose, a strain with a gal1-209 mutation was employed; this mutation disables the galactokinase function but retains the regulatory function for induction. The Escherichia coli lacZ gene (encoding beta-galactosidase) is functional in K. lactis and was used as the reporter gene downstream of the LAC4 promoter on a multicopy plasmid. The gal1-209 strain exhibited several unexpected phenomena, including partial consumption of the inducer galactose (although at a much slower rate relative to GAL1 strains) and growth inhibition at high concentrations of galactose. These unusual characteristics, however, did not prevent the successful construction of a strong gratuitous induction system. Due to the low rate of inducer consumption for the gratuitous strain, very low concentrations of galactose (1:20 galactose:glucose) resulted in high-level induction. Under these conditions, beta-galactosidase specific and volumetric activities were 4.2- and 5.5-fold higher, respectively, than those for the "GAL1" nongratuitous strain. This research demonstrated the improved productivity possible via LAC4 promoter-based gratuitous induction (and thus a more stable inducer concentration). The effects of various carbon source concentrations on growth and induction were also determined.
Transcriptome-wide identification of microRNA targets in rice (2010)

MicroRNA (miRNA)-guided target RNA expression is vital for a wide variety of biological processes in eukaryotes. Currently, miRBase (version 13) lists 142 and 353 miRNAs from Arabidopsis and rice (Oryza sativa), respectively. The integration of miRNAs in diverse biological networks relies upon the confirmation of their RNA targets. In contrast with the well-characterized miRNA targets that are cleaved in Arabidopsis, only a few such targets have been confirmed in rice. To identify small RNA targets in rice, we applied the 'degradome sequencing' approach, which globally identifies the remnants of small RNA-directed target cleavage by sequencing the 5' ends of uncapped RNAs. One hundred and sixty targets of 53 miRNA families (24 conserved and 29 rice-specific) and five targets of TAS3-small interfering RNAs (siRNAs) were identified. Surprisingly, an additional conserved target for miR398, which has not been reported so far, has been validated. Besides conserved homologous transcripts, 23 non-conserved genes for nine conserved miRNAs and 56 genes for 29 rice-specific miRNAs were also identified as targets. Besides miRNA targets, the rice degradome contained fragments derived from MIRNA precursors. A closer inspection of these fragments revealed a unique pattern distinct from siRNA-producing loci. This attribute can serve as one of the ancillary criteria for separating miRNAs from siRNAs in plants.
Friction and damage in the scratching of poly(n‐butyl acrylate) films (2002)

Abstract Scratch testing was performed on poly( n ‐butyl acrylate) (PnBA) films with a sapphire indenter with a spherical tip (76 μm in radius). For uncrosslinked PnBA film, the surface of which is sticky, the horizontal or scratching force decreases with decreasing normal load and has a residual value (∼ 6 mN) as the normal load approaches zero. The relation between the scratching force and normal load can be understood by finite element computation based on the Johnson–Kendall–Roberts theory under the assumption that the scratching force is proportional to the contact area, which depends on the normal load. With increasing driving speed, the scratching force shows a power relation with speed indicating a rate process. For crosslinked PnBA film, which behaves as an elastomer, the horizontal force approaches zero at zero normal load. Below a critical normal load, which depends on the thickness of the film, the crosslinked film recovers elastically after being scratched. Above the critical load, the film is damaged and, depending on its thickness, shows two distinct damage mechanisms. © 2002 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 40: 585–592, 2002; DOI 10.1002/polb.10117
CLINICAL APPLICATION OF ANGIOTENSIN II ANALOGUE (1-SARCOSINE, 8-ISOLEUCINE-ANGIOTENSIN II) (1974)

Angiotensin II (AII)の特異的拮抗物質としてA II analogueが開発され,現在最も強力,持続的物質として1-Sar, 8-Ile-A IIが紹介されている. A II analogueの人体応用により,ヒトにおける各種高血圧症,二次性aldostrone症等におけるA IIそのものの昇圧作用の意義について検索できる可能性があり,血圧調節におけるrenin-angiotensin-aldosterone系の解明にさらに多くの情報をもたらすものと考えられるが,現在までのところ本物質の人体応用にかんする報告はなされておらず,ここでは本物質の毒性試験施行後,臨床応用の可能性を検討した.健常人においては本薬50ng/kg/minの点滴により外因性A IIに有意な競合的拮抗を示し,その作用はほゞ5分後より出現し, 50～100分持続した.本薬の臨床応用を試み, i)本態性高血圧(正renin)6例では無反応, ii)低renin性の本態性高血圧およびCushing症候群の1例では昇圧, iii)悪性高血圧症3例のうち高renin,発症後1年未満の1例では有意に降下,他の正renin発症後10年以上の2例では無反応, iii)偏側腎血管性または腎硬塞性高血圧症6例(高renin)のうち発症後1年の1例は有意に降下, 3年の1例は軽度降下したが他の慢性例は高reninにもかかわらず無反応であつた.腹水を伴う肝硬変の1例では正常血圧からさらに著明な血圧降下が認められ,本病態におけるA II上昇が血圧維持に重なる意義を持つているものと考えられる,本薬は高血圧症の診断のみならず治療面への応用も期待される.
TLR9−/− and TLR9+/+ mice display similar immune responses to a DNA vaccine (2004)

Plasmid DNA continues to attract interest as a potential vaccine-delivery vehicle. However, the mechanisms whereby immune responses are elicited by plasmids are not fully understood. Although there have been suggestions regarding the importance of CpG motifs in plasmid immunogenicity, the molecular mechanisms by which CpG motifs enhance immune responses to DNA vaccines are not well understood. As Toll-like receptor 9-deficient (TLR9-/-) mice fail to respond to the adjuvant effects of CpG oligonucleotides, we used these mice to determine the effect of CpG motifs in plasmids used for DNA immunization. In the study described below, we report that DNA immunization was as effective in eliciting antigen-specific antibody and at stimulating antigen-specific interferon-gamma (IFN-gamma)-secreting cells in TLR9-/- mice as in TLR9+/+ mice. This study illustrates that DNA vaccines elicit immune responses by multiple mechanisms and demonstrates that TLR9 is not essential for the induction of immune responses following DNA immunization.
Universal Vaccine Based on Ectodomain of Matrix Protein 2 of Influenza A: Fc Receptors and Alveolar Macrophages Mediate Protection (2010)

The ectodomain of matrix protein 2 (M2e) of influenza A virus is an attractive target for a universal influenza A vaccine: the M2e sequence is highly conserved across influenza virus subtypes, and induced humoral anti-M2e immunity protects against a lethal influenza virus challenge in animal models. Clinical phase I studies with M2e vaccine candidates have been completed. However, the in vivo mechanism of immune protection induced by M2e-carrier vaccination is unclear. Using passive immunization experiments in wild-type, FcRγ(-/-), FcγRI(-/-), FcγRIII(-/-), and (FcγRI, FcγRIII)(-/-) mice, we report in this study that Fc receptors are essential for anti-M2e IgG-mediated immune protection. M2e-specific IgG1 isotype Abs are shown to require functional FcγRIII for in vivo immune protection but other anti-M2e IgG isotypes can rescue FcγRIII(-/-) mice from a lethal challenge. Using a conditional cell depletion protocol, we also demonstrate that alveolar macrophages (AM) play a crucial role in humoral M2e-specific immune protection. Additionally, we show that adoptive transfer of wild-type AM into (FcγRI, FcγRIII)(-/-) mice restores protection by passively transferred anti-M2e IgG. We conclude that AM and Fc receptor-dependent elimination of influenza A virus-infected cells are essential for protection by anti-M2e IgG.
Architecture Study for a Bare-Metal Direct Conversion Radar FPGA Testbench (2021)

As high-bandwidth all-digital radar platforms become increasingly prevalent, new signal processing techniques need to be explored to handle real-time processing of high-bandwidth data streams. The high bandwidth data streams produced by direct-sampling architectures present particularly difficult implementation requirements. To validate DSP algorithms for direct-conversion architectures with real measurements, testbenches need to be constructed with significantly different requirements than in other RF applications such as communication systems. One common usage of the term "testbenches" refers to pieces of code used during FPGA or ASIC simulation that employ specific clocks, interfacing to other devices, data input/output, etc. to validate an FPGA design. In this paper, a hardware testbench is discussed with a goal of providing the framework necessary to validate the design of DSP algorithms. Consequently, a modular radar signal processing testbench architecture is provided, including selected key implementation details and challenges. In brief, this paper's technical contribution is a system architecture for a high-bandwidth radar testbench, and its results section provides the resource utilization and floorplanning for a Virtex 7 FPGA.
Power-efficient downlink communication using large antenna arrays: The doughnut channel (2012)

Large antenna arrays at the base station can facilitate power efficient single user downlink communication due to the inherent array power gain, i.e., under an average only total transmit power constraint, for a fixed desired information rate, the required total transmit power can be reduced by increasing the number of base station antennas (e.g. with i.i.d. fading, the required total transmit power can be reduced by roughly 3 dB with every doubling in the number of base station antennas, i.e., an O(N) array power gain can be achieved with N antennas). However, in practice, building power efficient large antenna arrays would require power efficient amplifiers/analog RF components. With current technology, highly linear power amplifiers generally have low power efficiency, and therefore linearity constraints on power amplifiers must be relaxed. Under such relaxed linearity constraints, the transmit signal that suffers the least distortion is a signal with constant envelope (CE). In this paper, we consider a single user Gaussian multiple-input single-output (MISO) downlink channel where the signal transmitted from each antenna is constrained to have a constant envelope (i.e., for every channel-use the amplitude of the signal transmitted from each antenna is constant, irrespective of the channel realization). We show that under such a per-antenna CE constraint, the complex noise-free received signal lies in the interior of a “doughnut” shaped region in the complex plane. The per-antenna CE constrained MISO channel is therefore equivalent to a doughnut channel, i.e., a single-input single-output (SISO) AWGN channel where the channel input is constrained to lie inside a “doughnut” shaped region. Using this equivalence, we analytically compute a closed-form expression for an achievable information rate under the per-antenna CE constraint. We then show that, for a broad class of fading channels (i.i.d. and direct-line-of-sight (DLOS)), even under the more stringent per-antenna CE constraint (compared to the average only total power constraint), an O(N) array power gain can still be achieved with N base station antennas. We also show that with N ≫1, compared to the average only total transmit power constrained channel, the extra total transmit power required under the per-antenna CE constraint, to achieve a desired information rate is small and bounded for a broad class of fading channels (i.i.d. and DLOS). We also propose novel CE precoding algorithms. The analysis and algorithms presented are general and therefore applicable to conventional systems with a small number of antennas. Analytical results are supported with numerical results for the i.i.d. Rayleigh fading channel.
C. elegans Agrin Is Expressed in Pharynx, IL1 Neurons and Distal Tip Cells and Does Not Genetically Interact with Genes Involved in Synaptogenesis or Muscle Function (2007)

Agrin is a basement membrane protein crucial for development and maintenance of the neuromuscular junction in vertebrates. The C. elegans genome harbors a putative agrin gene agr-1. We have cloned the corresponding cDNA to determine the primary structure of the protein and expressed its recombinant fragments to raise specific antibodies. The domain organization of AGR-1 is very similar to the vertebrate orthologues. C. elegans agrin contains a signal sequence for secretion, seven follistatin domains, three EGF-like repeats and two laminin G domains. AGR-1 loss of function mutants did not exhibit any overt phenotypes and did not acquire resistance to the acetylcholine receptor agonist levamisole. Furthermore, crossing them with various mutants for components of the dystrophin-glycoprotein complex with impaired muscle function did not lead to an aggravation of the phenotypes. Promoter-GFP translational fusion as well as immunostaining of worms revealed expression of agrin in buccal epithelium and the protein deposition in the basal lamina of the pharynx. Furthermore, dorsal and ventral IL1 head neurons and distal tip cells of the gonad arms are sources of agrin production, but no expression was detectable in body muscles or in the motoneurons innervating them. Recombinant worm AGR-1 fragment is able to cluster vertebrate dystroglycan in cultured cells, implying a conservation of this interaction, but since neither of these proteins is expressed in muscle of C. elegans, this interaction may be required in different tissues. The connections between muscle cells and the basement membrane, as well as neuromuscular junctions, are structurally distinct between vertebrates and nematodes.
A new dynamic PV firming control algorithm using grid-tied three-port micro-converter (2018)

With the demand increase for electricity, the ever-increasing awareness of environmental issues, coupled with rolling blackouts, the role of renewable energy generation will become even more significant. Instead of the traditional high-power energy storage and management system at the utility scale to implement PV firming, this paper proposes a new PV (panel-level) firming control algorithm using grid-tied three-port bidirectional converter. The topology with the control algorithm and PV intermittency data collected in real time are discussed and analyzed in MATLAB/SIMULINK. The preliminary experimental results verify the proposed PV firming algorithm.
Flexure Mechanisms with Variable Stiffness and Damping Using Layer Jamming (2019)

Flexures provide precise motion control without friction or wear. Variable impedance mechanisms enable adaptable and robust interactions with the environment. This paper combines the advantages of both approaches through layer jamming. Thin sheets of complaint material are encased in an airtight envelope, and when connected to a vacuum, the bending stiffness and damping increase dramatically. Using layer jamming structures as flexure elements leads to mechanical systems that can actively vary stiffness and damping. This results in flexure mechanisms with the versatility to transition between degrees of freedom and degrees of constraint and to tune impact response. This approach is used to create a 2-DOF, jamming-based, tunable impedance robotic wrist that enables passive hybrid force/position control for contact tasks. Keywords: Compliant Joint/Mechanism, Compliance and Impedance Control, Mechanism Design.
ChemInform Abstract: THE NORBORNADIENE ROUTE TO PROSTAGLANDIN I2 AND OTHER PROSTAGLANDINS. PREPARATION AND REARRANGEMENT OF 7‐SUBSTITUTED NORBORNADIENES (1983)

Abstract Das tert.‐Butoxy‐norbomadien (II) reagiert über das entsprechende Chlorid mit (I) zum Acetylenderivat (III), das über (IV) zum Aldehyd (V) führt.
Controlled Assembly and Anomalous Thermal Expansion of Ultrathin Cesium Lead Bromide Nanoplatelets (2023)

Quantum confined lead halide perovskite nanoplatelets are anisotropic materials displaying strongly bound excitons with spectrally pure photoluminescence. We report the controlled assembly of CsPbBr<sub>3</sub> nanoplatelets through varying the evaporation rate of the dispersion solvent. We confirm the assembly of superlattices in the face-down and edge-up configurations by electron microscopy, as well as X-ray scattering and diffraction. Polarization-resolved spectroscopy shows that superlattices in the edge-up configuration display significantly polarized emission compared to face-down counterparts. Variable-temperature X-ray diffraction of both face-down and edge-up superlattices uncovers a uniaxial negative thermal expansion in ultrathin nanoplatelets, which reconciles the anomalous temperature dependence of the emission energy. Additional structural aspects are investigated by multilayer diffraction fitting, revealing a significant decrease in superlattice order with decreasing temperature, with a concomitant expansion of the organic sublattice and increase of lead halide octahedral tilt.
A Novel Multiplex PCR Discriminates Bacillus anthracis and Its Genetically Related Strains from Other Bacillus cereus Group Species (2015)

Anthrax is an important zoonotic disease worldwide that is caused by Bacillus anthracis, a spore-forming pathogenic bacterium. A rapid and sensitive method to detect B. anthracis is important for anthrax risk management and control in animal cases to address public health issues. However, it has recently become difficult to identify B. anthracis by using previously reported molecular-based methods because of the emergence of B. cereus, which causes severe extra-intestinal infection, as well as the human pathogenic B. thuringiensis, both of which are genetically related to B. anthracis. The close genetic relation of chromosomal backgrounds has led to complexity of molecular-based diagnosis. In this study, we established a B. anthracis multiplex PCR that can screen for the presence of B. anthracis virulent plasmids and differentiate B. anthracis and its genetically related strains from other B. cereus group species. Six sets of primers targeting a chromosome of B. anthracis and B. anthracis-like strains, two virulent plasmids, pXO1 and pXO2, a bacterial gene, 16S rRNA gene, and a mammalian gene, actin-beta gene, were designed. The multiplex PCR detected approximately 3.0 CFU of B. anthracis DNA per PCR reaction and was sensitive to B. anthracis. The internal control primers also detected all bacterial and mammalian DNAs examined, indicating the practical applicability of this assay as it enables monitoring of appropriate amplification. The assay was also applied for detection of clinical strains genetically related to B. anthracis, which were B. cereus strains isolated from outbreaks of hospital infections in Japan, and field strains isolated in Zambia, and the assay differentiated B. anthracis and its genetically related strains from other B. cereus group strains. Taken together, the results indicate that the newly developed multiplex PCR is a sensitive and practical method for detecting B. anthracis.
Glycosphingolipid synthesis inhibition limits osteoclast activation and myeloma bone disease (2015)

Glycosphingolipids (GSLs) are essential constituents of cell membranes and lipid rafts and can modulate signal transduction events. The contribution of GSLs in osteoclast (OC) activation and osteolytic bone diseases in malignancies such as the plasma cell dyscrasia multiple myeloma (MM) is not known. Here, we tested the hypothesis that pathological activation of OCs in MM requires de novo GSL synthesis and is further enhanced by myeloma cell-derived GSLs. Glucosylceramide synthase (GCS) inhibitors, including the clinically approved agent N-butyl-deoxynojirimycin (NB-DNJ), prevented OC development and activation by disrupting RANKL-induced localization of TRAF6 and c-SRC into lipid rafts and preventing nuclear accumulation of transcriptional activator NFATc1. GM3 was the prevailing GSL produced by patient-derived myeloma cells and MM cell lines, and exogenous addition of GM3 synergistically enhanced the ability of the pro-osteoclastogenic factors RANKL and insulin-like growth factor 1 (IGF-1) to induce osteoclastogenesis in precursors. In WT mice, administration of GM3 increased OC numbers and activity, an effect that was reversed by treatment with NB-DNJ. In a murine MM model, treatment with NB-DNJ markedly improved osteolytic bone disease symptoms. Together, these data demonstrate that both tumor-derived and de novo synthesized GSLs influence osteoclastogenesis and suggest that NB-DNJ may reduce pathological OC activation and bone destruction associated with MM.