gebhart/patrepeval huggingface dataset:
Multiparameter flow cytometry in the evaluation of myelodysplasia: Analytical issues (2022)

Multiparameter flow cytometry (MFC) is one of the essential ancillary methods in bone marrow (BM) investigation of patients with cytopenia and suspected myelodysplastic syndrome (MDS). MFC can also be applied in the follow-up of MDS patients undergoing treatment. This document summarizes recommendations from the International/European Leukemia Net Working Group for Flow Cytometry in Myelodysplastic Syndromes (ELN iMDS Flow) on the analytical issues in MFC for the diagnostic work-up of MDS. Recommendations for the analysis of several BM cell subsets such as myeloid precursors, maturing granulocytic and monocytic components and erythropoiesis are given. A core set of 17 markers identified as independently related to a cytomorphologic diagnosis of myelodysplasia is suggested as mandatory for MFC evaluation of BM in a patient with cytopenia. A myeloid precursor cell (CD34<sup>+</sup> CD19<sup>-</sup> ) count >3% should be considered immunophenotypically indicative of myelodysplasia. However, MFC results should always be evaluated as part of an integrated hematopathology work-up. Looking forward, several machine-learning-based analytical tools of interest should be applied in parallel to conventional analytical methods to investigate their usefulness in integrated diagnostics, risk stratification, and potentially even in the evaluation of response to therapy, based on MFC data. In addition, compiling large uniform datasets is desirable, as most of the machine-learning-based methods tend to perform better with larger numbers of investigated samples, especially in such a heterogeneous disease as MDS.
Well-Known Community Policy Behavior (2019)

Well-Known BGP Communities are manipulated differently across various
current implementations; resulting in difficulties for operators.
Network operators should deploy consistent community handling across
their networks while taking the inconsistent behaviors from the
various BGP implementations into consideration.. This document
recommends specific actions to limit future inconsistency, namely BGP
implementors must not create further inconsistencies from this point
forward.
Streaming Compressive Sensing for high-speed periodic videos (2010)

The ability of Compressive Sensing (CS) to recover sparse signals from limited measurements has been recently exploited in computational imaging to acquire high-speed periodic and near-periodic videos using only a low-speed camera with coded exposure and intensive off-line processing. Each low-speed frame integrates a coded sequence of high-speed frames during its exposure time. The high-speed video can be reconstructed from the low-speed coded frames using a sparse recovery algorithm. This paper presents a new streaming CS algorithm specifically tailored to this application. Our streaming approach allows causal on-line acquisition and reconstruction of the video, with a small, controllable, and guaranteed buffer delay and low computational cost. The algorithm adapts to changes in the signal structure and, thus, outperforms the off-line algorithm in realistic signals.
Corrigendum: Synthesis of [18F]BODIPY: Bifunctional Reporter for Hybrid Optical/Positron Emission Tomography Imaging (2012)

While this work was in progress, a team led by Z. Li, P. Conti, and F. Gabba reported an alternative approach to [ 18 F]-BODIPY dyes. In the same paper, they also demonstrated the positron emission tomography/fluorescence dual modality imaging capabilities of these radiolabeled dyes.
Therapeutic Targeting of Syk in Autoimmune Diabetes (2010)

In APCs, the protein tyrosine kinase Syk is required for signaling of several immunoreceptors, including the BCR and FcR. We show that conditional ablation of the syk gene in dendritic cells (DCs) abrogates FcgammaR-mediated cross priming of diabetogenic T cells in RIP-mOVA mice, a situation phenocopied in wild-type RIP-mOVA mice treated with the selective Syk inhibitor R788. In addition to blocking FcgammaR-mediated events, R788 also blocked BCR-mediated Ag presentation, thus broadly interrupting the humoral contributions to T cell-driven autoimmunity. Indeed, oral administration of R788 significantly delayed spontaneous diabetes onset in NOD mice and successfully delayed progression of early-established diabetes even when treatment was initiated after the development of glucose intolerance. At the DC level, R788 treatment was associated with reduced insulin-specific CD8 priming and decreased DC numbers. At the B cell level, R788 reduced total B cell numbers and total Ig concentrations. Interestingly, R788 increased the number of IL-10-producing B cells, thus inducing a tolerogenic B cell population with immunomodulatory activity. Taken together, we show by genetic and pharmacologic approaches that Syk in APCs is an attractive target in T cell-mediated autoimmune diseases such as type 1 diabetes.
PCI Express 6.0 Specification: A Low-Latency, High-Bandwidth, High-Reliability, and Cost-Effective Interconnect With 64.0 GT/s PAM-4 Signaling (2020)

PCI Express (PCIe) specification has been doubling the data rate every generation in a backward compatible manner every two to three years. PCIe 6.0 specification will adopt PAM-4 signaling at 64.0 GT/s for maintaining the same channel reach of prior generations. A forward error correction (FEC) mechanism will offset the high BER of PAM-4. We propose a new flit-based approach with a lightweight, low-latency FEC coupled with a strong cyclic redundancy check (CRC) and a low-latency link-level retry mechanism to meet the stringent low-latency, high-bandwidth, and high-reliability goals. We also present a new low-power state that ensures power consumption is proportional to bandwidth usage without impacting the traffic flow.
Advances in the oral delivery of proteins (1999)

The efforts to develop oral formulations of protein drugs have been intensified in recent years as a consequence of the increasing number of protein-based therapeutics that are being identified. Yet, due to the substantial and unprecedented challenges that the oral delivery of proteins presents, advances in this field have been relatively slow. Nonetheless, several oral formulations of proteins are already in clinical trials and others are expected to start clinical testing in the immediate future. This review discusses the recent developments and the patent literature in the area of oral delivery of protein therapeutics.
Revisiting network support for RDMA (2018)

The advent of RoCE (RDMA over Converged Ethernet) has led to a significant increase in the use of RDMA in datacenter networks. To achieve good performance, RoCE requires a lossless network which is in turn achieved by enabling Priority Flow Control (PFC) within the network. However, PFC brings with it a host of problems such as head-of-the-line blocking, congestion spreading, and occasional deadlocks. Rather than seek to fix these issues, we instead ask: is PFC fundamentally required to support RDMA over Ethernet?
Multiplexed Chemical Sensing CMOS Imager (2022)

A miniaturized and multiplexed chemical sensing technology is urgently needed to empower mobile devices and robots for various new applications such as mobile health and Internet of Things. Here, we show that a complementary metal-oxide-semiconductor (CMOS) imager can be turned into a multiplexed colorimetric sensing chip by coating micron-scale sensing spots on the CMOS imager surface. Each sensing spot contains nanocomposites of colorimetric sensing probes and silica nanoparticles that enhance sensing signals by several orders of magnitude. The sensitivity is spot-size-invariant, and high-performance gas sensing can be achieved on sensing spots as small as ∼10 μm. This great scalability combined with millions of pixels of a CMOS imager offers a promising platform for highly integrated chemical sensors. To prove its compatibility with mobile electronics, we have built a smartphone accessory based on this chemical CMOS sensor and demonstrated that personal health management can be achieved through the detection of gaseous biomarkers and pollutants. We anticipate that this new platform will pave the way for the widespread application of chemical sensing in mobile electronics and wearable devices.
On the Distribution of Boolean Function Nonlinearity (2008)

Nonlinearity is the number of bits which must change in the truth table of a Boolean function to reach the closest affine function. It may be expressed through the maximum of the absolute value of a component in the function's Walsh–Hadamard transform. Concentration of nonlinearity is proved. The derived bounds on the concentration point and tails of the distribution are tighter than the earlier known ones.
Signal Processing for Assisted Living: Developments and Open Problems [From the Guest Editors] (2016)

The six articles in this special section provide a synopsis of the emerging area of signal processing for assisted living, including the most recent developments as well as interesting open problems at the forefront of the current research. The articles demonstrate the role of signal processing in addressing key challenges and solving pressing problems encountered in assisted living applications related to various sensing modalities.
Effect of Thread and Bearing Friction Coefficients on the Self-Loosening of Preloaded Countersunk-Head Bolts Under Periodic Transverse Excitation (2010)

A nonlinear mathematical model is developed for studying the self-loosening behavior of preloaded countersunk threaded fasteners that are subjected to cyclic transverse loads. Torque components acting on the bolt are divided into pitch and resistance torque components; the net torque determines whether or not the bolt will rotate loose under the external excitation. The accumulation of the differential amount of loosening rotation increments is converted into the gradual loss of the bolt tension/clamp load. Although the loosening model incorporates several system variables, this study is focused on investigating the effect of thread and bearing friction coefficients on the loosening of fasteners with coarse and fine threads. Model prediction of the self-loosening behavior is experimentally validated.
Decoupling and recoupling; Nitrogen-14 NMR; Dynamic nuclear polarization, Bodenhausen G, (2009)

International audience
Generation of calcium waves in living cells by pulsed-laser-induced photodisruption (2001)

Here, we present an optical technique that can induce waves of calcium ion concentration in live biological cells. Ca2+ waves were induced by femtosecond pulsed-laser illumination. Living HeLa cells were exposed to focused 140 fs pulses of 780 nm wavelength at 30 mW average power. Ca2+ waves were imaged by fluorescence and were observed to propagate from the laser focal point inside the cell. Photoinduced generation of Ca2+ waves can be performed at any point inside the cell, an improvement over previous mechanical or biochemical stimulation techniques.
Multiple focused image hologram model (1986)

A model describing the image irradiance of two images produced by a multiple grating reflection type focusing element is developed. The gratings may be formed simultaneously or sequentially, and the recording medium can change thickness during processing. In the algorithm, the spot size and location of the image are first calculated by ray tracing from each surface grating in the multiplexed grating element. The efficiencies of these rays are then computed with a multiple grating model which allows for coupling between the fields in the grating volume. Finally, the efficiency of each ray is weighted by a factor corresponding to the intensity profile of the reconstruction beam. The sum of these weighted rays per unit area in the image plane determines the relative image irradiance. A comparison is then made between the image irradiance produced by experimental multiple focused elements and the calculated irradiance pattern. The model predicts the relative profile and the size of the irradiance patterns.
Determination of Thermoset Resin Cross-link Architecture by Gel Permeation Chromatography (1984)

Gel permeation chromatography is the method of choice for analysis of thermoplastic resin systems. Corrected for imperfect resolution, chromatogram interpretation yields accurate molecular descriptions, including theoretical, kinetic distributions (1,2). The current research is designed to extend the utility of this analytical tool to the analysis of thermoset resins. Kinetic mechanisms (3) are such that low molecular weight species are present in a cured resin; in fact, the molar concentration of dimers, trimers, etc. usually exceeds that for higher molecular weight species. An exception is a Poisson distribution, but oligomeric species are still abundant. If a cured thermoset resin is prepared such that a large surface area to volume ratio is achieved, solvent leaching provides an effective method for sample preparation. Analysis of extracts (4,5) provides data descriptive of monomeric content and oligomeric, molecular distributions. Such extracts contain definitive information with respect to the extent of cure as well as a
Photocatalytic Suzuki Coupling Reaction Using Conjugated Microporous Polymer with Immobilized Palladium Nanoparticles under Visible Light (2015)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTPhotocatalytic Suzuki Coupling Reaction Using Conjugated Microporous Polymer with Immobilized Palladium Nanoparticles under Visible LightZi Jun Wang, Saman Ghasimi, Katharina Landfester, and Kai A. I. Zhang*View Author Information Max Planck Institute for Polymer Research, D-55128 Mainz, Germany*E-mail: [email protected]Cite this: Chem. Mater. 2015, 27, 6, 1921–1924Publication Date (Web):March 2, 2015Publication History Received28 January 2015Revised28 February 2015Published online6 March 2015Published inissue 24 March 2015https://pubs.acs.org/doi/10.1021/acs.chemmater.5b00516https://doi.org/10.1021/acs.chemmater.5b00516rapid-communicationACS PublicationsCopyright © 2015 American Chemical SocietyRequest reuse permissionsArticle Views5732Altmetric-Citations143LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Coupling reactions,Electromagnetic radiation,Metal nanoparticles,Palladium,Photocatalysts Get e-Alerts
Erratum to: Effects of multi-species probiotic supplementation on alcohol metabolism in rats (2021)

In the article by Lim et al. published in Journal of Microbiology 2021; 59, 417–425, did not include acknowledgments or conflict of interest.
Gene expression study related with the intrinsic pathway of apoptosis in bladder cancer by real-time PCR technique (2013)

We examined the expression of anti-apoptotic genes (XIAP and Bcl-2) and apoptotic genes (cytochrome c, caspase-9, Apaf-1) in tissue samples of patients with superficial bladder cancer. Thirty-two bladder cancer tissue samples (8 papillary urothelial neoplasm of low malignant potential, 10 low-grade, and 14 high-grade) and 8 normal bladder tissue samples from necropsy were used for the study of gene expression by real-time PCR analysis. Analysis of the expression of apoptotic gene constituents of an apoptosome demonstrated an increase in Apaf-1 expression in the three tumor grades when compared with the control (P < 0.01, P < 0.05, and P < 0.01), low expression of caspase-9 in all groups (P < 0.05), and an increase in cytochrome c expression in all tumor grades in relation to the control, although without statistically significant difference. The expression of anti-apoptotic genes revealed an increase in XIAP expression in all tumor grades in relation to the control, although without statistically significant difference, and low expression of Bcl-2 in all tumor grades and the control (P < 0.05). The results proved that there is low evidence of apoptotic activity by the intrinsic pathway, demonstrated by the low expression of caspase-9 and considerable increase in XIAP expression, which may render these genes potential therapeutic targets in bladder cancer treatment.
Pathways for ATP release by bovine ciliary epithelial cells, the initial step in purinergic regulation of aqueous humor inflow (2010)

ATP release by nonpigmented (NPE) and pigmented (PE) ciliary epithelial cells is the enabling step in purinergic regulation of aqueous humor formation, but the release pathways are unknown. We measured ATP release from primary cultures of bovine mixed NPE and PE (bCE) cells and transformed bovine NPE and PE cells, using the luciferin-luciferase reaction. Hypotonicity-triggered bCE ATP release was inhibited by the relatively selective blocker of pannexin-1 (PX1) hemichannels (probenecid, 1 mM, 47 ± 2%), by a connexin inhibitor (heptanol, 1 mM, 49 ± 4%), and by an inhibitor of vesicular release (bafilomycin A1, 25 ± 2%), but not by the P2X(7) receptor (P2RX(7)) antagonist KN-62. Bafilomycin A1 acts by reducing the driving force for uptake of ATP from the cytosol into vesicles. The reducing agent dithiothreitol reduced probenecid-blockable ATP release. Similar results were obtained with NPE and PE cell lines. Pannexins PX1-3, connexins Cx43 and Cx40, and P2RX(7) were identified in native cells and cell lines by RT-PCR. PX1 mRNA expression was confirmed by Northern blots; its quantitative expression was comparable to that of Cx43 by real-time PCR. Heterologous expression of bovine PX1 in HEK293T cells enhanced swelling-activated ATP release, inhibitable by probenecid. We conclude that P2RX(7)-independent PX1 hemichannels, Cx hemichannels, and vesicular release contribute comparably to swelling-triggered ATP release. The relatively large response to dithiothreitol raises the possibility that the oxidation-reduction state is a substantial regulator of PX1-mediated ATP release from bovine ciliary epithelial cells.
Suspended Polymer Photonic Crystal Networks (2012)

We demonstrate a suspended polymer photonic crystal architecture with an in-plane photonic bandgap and enabling high-Q nanocavities. Employing this architecture, we implement a waveguide-coupled cavity drop filter with sub-nanometer spectral resolution.
Water Oxidation with Cobalt‐Loaded Linear Conjugated Polymer Photocatalysts (2020)

The first examples of linear conjugated organic polymer photocatalysts that produce oxygen from water after loading with cobalt and in the presence of an electron scavenger are reported. The oxygen evolution rates, which are higher than for related organic materials, can be rationalized by a combination of the thermodynamic driving force for water oxidation, the light absorption of the polymer, and the aqueous dispersibility of the relatively hydrophilic polymer particles. We also used transient absorption spectroscopy to study the best performing system and we found that fast oxidative quenching of the exciton occurs (picoseconds) in the presence of an electron scavenger, minimizing recombination.
Identification of Multilinear Forms with the Tensorial Kalman Filter (2021)

The multilinear system identification problem is usually approached with tensors. Recent works have addressed this problem using the well-known Wiener filter, as well as some conventional adaptive algorithms, such as the least-mean-square and recursive least-squares. In this paper, we derive a tensorial Kalman filter designed for the identification of multilinear forms. Furthermore, based on steady-state approximations, we also develop a simplified version of this algorithm, with lower computational complexity. Experimental results support the theoretical findings, highlighting the good performance of the proposed solutions.
Synthesis and optimisation of new wideband symmetrical six‐port junction (2016)

In this study, the authors propose a new approach for the synthesis of wideband symmetrical six‐port junctions. The suggested approach is based on a novel microstrip topology that can be optimised using genetic algorithm. The proposed approach has been numerically validated through the synthesis of single and double cell six‐port junctions. As an experimental validation single and double cell six‐port junctions with centre frequencies of 2.5 and 5.3 GHz, respectively, have been realised and measured. The obtained experimental results have revealed the performance of the proposed synthesis approach, its robustness and its superiority compared to the conventional empirical design techniques.
Prevalence of RFC1 -Mediated Spinocerebellar Ataxia in a United States Ataxia Cohort (2019)

ABSTRACT Objective Repeat expansions in RFC1 and DAB1 have recently been identified as causing cerebellar ataxia, neuropathy, and vestibular areflexia syndrome (CANVAS) and spinocerebellar ataxia 37 (SCA37), respectively. We evaluated the prevalence of these repeat-expansions in an undiagnosed ataxia cohort from the United States. Methods A cohort of 596 patients with undiagnosed familial or sporadic cerebellar ataxia were evaluated at a tertiary referral ataxia center and excluded for common genetic causes of cerebellar ataxia. Patients were then screened for the presence of pathogenic repeat expansions in RFC1 (AAGGG) and DAB1 (ATTTC) using fluorescent repeat primed polymerase chain reaction (RP-PCR). Two additional undiagnosed ataxia cohorts from different centers, totaling 96 and 13 patients respectively, were subsequently screened for RFC1 resulting in a combined 705 subjects tested. Results In the initial cohort, 42 samples were identified with one expanded allele in the RFC1 gene (7.0%), and 9 had two expanded alleles (1.5%). For the additional cohorts, we found 12 heterozygous samples (12.5%) and 7 biallelic samples (7.3%) in the larger cohort, and 1 heterozygous sample (7.7%) and 3 biallelic samples (23%) in the second. In total, 19 patients were identified with biallelic repeat expansions in RFC1 (2.7%). Of these 19 patients, 6 (32%) had a clinical diagnosis of CANVAS, 10 had cerebellar ataxia with neuropathy (53%), and 3 had spinocerebellar ataxia (16%). No patients were identified with expansions in the DAB1 gene. Conclusion In a large undiagnosed ataxia cohort from the United States, biallelic pathogenic repeat expansion in RFC1 was observed in 2.7%. Testing should be strongly considered in ataxia patients, especially those with CANVAS or neuropathy.
Facile synthesis, assembly, and immobilization of ordered arrays of monodisperse magnetic nanoparticles on silicon substrates (2008)

This paper outlines the preparation of monodisperse MnFe(2)O(4) nanoparticles modified with omega-alkenyl moieties in a one-pot reaction, requiring no ligand-exchange step, followed by deposition of the resulting surfactant-coated nanoparticles onto a hydrogen-terminated silicon (111) wafer and covalent anchoring to the surface via UV-initiated bonding, creating a stable two-dimensional array of monodisperse magnetic nanoparticles.