gebhart/patrepeval huggingface dataset:
Abstract 2692: Dendrimer delivery of RNA based Twist1 inhibitors to reduce breast cancer cell metastasis. (2013)

Abstract Breast cancer in women is a leading cause of cancer related deaths in the United States with metastatic disease being the chief cause of mortality. Metastatic breast cancer tumors are often found in their later stages making them difficult to remove or treat. Inhibition of tumor cell dispersion is therefore a key component to a reduction in mortality rates among women with breast cancer. Epithelial-Mesenchymal Transition (EMT) is the process by which cancer cells downregulate proteins associated with cell to cell adhesion (e.g. E-cadherin) resulting in cells that are able to mobilize. Breast cancer cells are known to commandeer the EMT process through overexpression of Twist1, thus allowing the cells to metastasize beyond the primary tumor. Twist1 therefore serves as an excellent therapeutic target whose disruption would result in fewer metastatic cancer cells and correspondingly reduce cancer mortality. Twist1 is also a desirable target because it is almost nonexistent in adult tissues and thus its silencing would have minimal side effects. Our lab has elected to use an siRNA mediated gene silencing approach to decrease the expression levels of Twist1 in two highly invasive breast cancer cell lines (SUM 1315 and MCF7-Tw). Due to their fragile nature, siRNA molecules are often difficult to deliver at therapeutic levels. We have overcome these delivery limitations through the optimization and use of Poly (amidoamine) (PAMAM) dendrimers. We hypothesize that suppression of the activity of Twist1 via dendrimer-delivered Twist1 siRNA will inhibit metastatic behavior of aggressive breast cancer cells. Here we demonstrate successful delivery of Cy3-labled siRNA using two different dendrimers (Generation 5 and a modified Generation 3) with transfection efficiency results exceeding those of Lipofectamine 2000 and with far less toxicity to cells. We also show up to a 90% reduction (lasting at least 4 days) in Twist1 expression using Western Blot and qPCR analysis. Phenotypically we were able to appreciate a significant decrease in the invasive nature of the breast cancer cells using migration/invasion assays. Taken together these results demonstrate successful knockdown of Twist1 using siRNA dendrimer complexes resulting in an altered cellular phenotype. These data will serve as a foundation for optimization of future in vivo experiments with both orthotopic and metastatic breast cancer models. Citation Format: James B. Finlay, Cai M. Roberts, Carlotta A. Glackin. Dendrimer delivery of RNA based Twist1 inhibitors to reduce breast cancer cell metastasis. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 2692. doi:10.1158/1538-7445.AM2013-2692
You Can't Win Them All (2012)

Losses are a part of the trading business. Therefore, learning to accept losses, deal with it, and minimize losses is the most important factor in determining the success as a trader. Individual losses can happen for any number of reasons in the commodity futures markets; however, there are three major causes a trader loses on a consistent basis: Leverage, gambling, and cutting losses. The key to successful losing, if one would allow for the slight contradiction in terms, is to be willing to take a loss whenever necessary, instead of trying to figure out how to turn a loser into a winner. The markets are strong or weak for a reason; either way, the market gives information; therefore, it is the job of the trader to pay attention to everything it is saying and to notice what it is not saying.
Photochemistry of Thiarubrine A and Other 1,2-Dithiins:  Formation of 2,6-Dithiabicyclo[3.1.0]hex-3-enes (1996)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTPhotochemistry of Thiarubrine A and Other 1,2-Dithiins: Formation of 2,6-Dithiabicyclo[3.1.0]hex-3-enesEric Block, Jon Page, John P. Toscano, Cun-Xiao Wang, Xing Zhang, Russell DeOrazio, Chuangxing Guo, Robert S. Sheridan, and G. H. Neil TowersView Author Information Departments of Chemistry State University of New York, Albany, New York 12222 University of Nevada, Reno, Nevada 89557 Johns Hopkins University, Baltimore, Maryland 21218 Department of Botany, University of British Columbia Vancouver, British Columbia, Canada V6T 1Z4 Cite this: J. Am. Chem. Soc. 1996, 118, 19, 4719–4720Publication Date (Web):May 15, 1996Publication History Received23 February 1996Published online15 May 1996Published inissue 1 January 1996https://doi.org/10.1021/ja960589vCopyright © 1996 American Chemical SocietyRequest reuse permissionsArticle Views304Altmetric-Citations27LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit Read OnlinePDF (119 KB) Get e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information SUBJECTS:Electromagnetic radiation,Irradiation,Isolation,Molecular structure,Thiophenes Get e-Alerts
New propagation mode of fiber fuse with a long-period damage track in hole-assisted fiber (HAF) (2010)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text N. Hanzawa, K. Kurokawa, K. Tsujikawa, K. Takenaga, S. Tanigawa, S. Matsuo, and S. Tomita, "New propagation mode of fiber fuse with a long-period damage track in hole-assisted fiber (HAF)," in Optical Fiber Communication Conference, OSA Technical Digest (CD) (Optica Publishing Group, 2010), paper OMO5. Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
Capstone project on speech morphing: A highlight to undergraduate education on signal processing (2016)

Signal processing is a core discipline in electrical and computer engineering (ECE). The mathematical language adopted in the classroom and the laboratory support, usually limited to verification, pose the risk of hiding the enormous practical connotations of the discipline. This paper suggests to strengthen the bridge between theory and practice by means of selected capstone projects, designed according to constructivist principles. The project detailed in this paper tackles a large number of fundamental aspects of the discipline through a storyline that captivates the student during its execution.
Supplemental Figure legends from CRM1 Inhibition Promotes Cytotoxicity in Ewing Sarcoma Cells by Repressing EWS-FLI1–Dependent IGF-1 Signaling (2023)

&lt;p&gt;Figure legends for supplemental figures S1-S6.&lt;/p&gt;
Clinical Commercialization of a Clonal Cell Line NK-92 - Leveraging Corporate Development (2004)

s for the 19th Annual Scientific Meeting of the International Society for Biological Therapy of Cancer, San Francisco, California, November 4-7, 2004: Other
Synthesis and Bioactivity of Ophiofuranones A and B (2022)

Ophiofuranones A and B, metabolites of the fungus <i>Ophiosphaerella korrae</i>, were synthesized in 16 steps and 12%/22% yield. The stereogenic centers were established by Sharpless dihydroxylations and epoxidation, the 1,3-dienes via Wittig or HWE olefinations. The rings were closed through Knoevenagel-type condensation and lactonization. The ophiofuranones proved nontoxic at relevant concentrations against tumor cells, fibroblasts, and various bacteria and fungi. Ophiofuranone A and the monocyclic precursors <b>4</b> were weakly active against microbial biofilms.
Bringing 5G into Rural and Low-Income Areas: Is It Feasible? (2017)

Nowadays, at least two billion people are experiencing a complete lack of wireless cellular network coverage. These users live in rural areas and low-income regions, where network operators are not keen to invest, mainly due to high capital expenditure and operational expenditure costs, as well as the scarcity of electricity from the grid. We tackle this challenge by proposing a 5G network explicitly designed to serve rural and low-income areas. Our solution investigates the possibility of mounting remote radio heads on top of unmanned aerial vehicles, as well as large cells (LCs) to increase the coverage range. In addition, 5G nodes are powered by solar panels and batteries. Preliminary results, obtained over three representative case studies located in Italy, Cook Islands, and Zimbabwe, show that providing connectivity in rural and low-income areas by means of the proposed 5G architecture is feasible. At the same time, we also show that the monthly subscription fee paid by the users can be kept sufficiently low, that is, less than €1/month in low-income areas, and around €11/month in rural regions.
Advances in Computational Imaging (2015)

Computational imaging uses new optics to capture a coded image, and an appropriate algorithm to decode the captured image. This approach of manipulating images before there are recorded and processing recorded images before they are presented has three key benefits. First, it enables us to implement imaging functionalities that would be difficult, if not impossible, to achieve using traditional imaging. Second, it can be used to significantly reduce the hardware complexity of an imaging system. Lastly, under appropriate imaging conditions, it allows us to break the limits of traditional imaging. In this talk, I’ll show recent examples of cameras that demonstrate these benefits.
Glycomic Analysis Reveals a Conserved Response to Bacterial Sepsis Induced by Different Bacterial Pathogens (2022)

Sepsis is an extreme inflammatory response to infection that occurs in the bloodstream and causes damage throughout the body. Glycosylation is known to play a role in immunity and inflammation, but the role of glycans in sepsis is not well-defined. Herein, we profiled the serum glycomes of experimental mouse sepsis models to identify changes induced by 4 different clinical bacterial pathogens (Gram-positive: <i>Streptococcus pneumoniae</i> and <i>Staphylococcus aureus</i>, Gram-negative: <i>Escherichia coli</i> and <i>Salmonella</i> Typhimurium) using our lectin microarray technology. We observed global shifts in the blood sera glycome that were conserved across all four species, regardless of whether they were Gram positive or negative. Bisecting GlcNAc was decreased upon sepsis and a strong increase in core 1/3 <i>O</i>-glycans was observed. Lectin blot analysis revealed a high molecular weight protein induced in sepsis by all four bacteria as the major cause of the core 1/3 <i>O</i>-glycan shift. Analysis of this band by mass spectrometry identified interalpha-trypsin inhibitor heavy chains (ITIHs) and fibronectin, both of which are associated with human sepsis. Shifts in the glycosylation of these proteins were observed. Overall, our work points toward a common mechanism for bacterially induced sepsis, marked by conserved changes in the glycome.
Monitoring acute rejection and inducing tolerance with IL-2/Fc in CTA (2010)

Jindal, Rishi MD; Zhang, D. MD, PhD; Unadkat, J. V. MD; Ng, T. BS; Wang, Y. MD; Weinstock, M. MD; Sucher, R. MD; Pulikkottil, B. J. MD; Lee, W. P.A. MD; Zheng, Xin Xiao MD Author Information
60% Power Reduction in Inductive-Coupling Inter-Chip Link by Current-Sensing Technique (2007)

The demand for low-power and high-speed chip-to-chip communication between stacked chips in a system in a package (SiP) is increasing day by day. We have proposed and investigated a novel current-sensing technique in an inductive-coupling inter-chip link. Our current-sensing technique reduces total power dissipation by 60% compared with the conventional voltage-sensing technique without sacrificing either data rate (1 Gbit/s per channel) or bit error rate (<10-12). Additionally, the voltage-sensing technique and the current-sensing technique are compared from the viewpoint of circuit topology and the immunity to device mismatch. From a circuit simulation, the current-sensing technique was shown to have higher immunity to device mismatch and can reduce the transmitter power compared with voltage-sensing technique. We have reached a conclusion that the current-sensing technique is very effective for low-power operation in an inductive-coupling inter-chip link.
Introduction and Overview (2014)

Infections caused by RNA viruses represent some of the most significant diseases of vertebrates in terms of both morbidity and economic impact. In addition to those with stable human reservoirs, RNA viruses are frequently responsible for zoonotic infections. Such infections account for some of the most feared and least understood infectious diseases. West Nile virus is an arthropod-transmitted virus whose natural reservoir is birds; it emerged in around 2000 as an important new cause of significant human neurological disease, including death, in North America. RNA viruses are clearly worthy of careful examination to understand mechanisms of disease pathogenesis, early and late immune responses, and immune evasion, and as such knowledge will help us develop new and more effective methods for controlling their spread or modifying their disease course. This introductory chapter of the book Cellular Signaling and Innate Immune Responses to RNA Virus Infections presents a brief insight into the organization of the book. The book is divided into two major parts. The first part focuses on the structure, interconnectivity, and mechanisms of activation of the pattern recognition receptors (PRRs)-initiated signaling pathways. The second part of this book examines the ways in which the major RNA virus families interact with these signaling pathways. The understanding of the specific pathways that will be gained from a review of the first part will help to place the diverse mechanisms of immune activation and evasion in context. Finally, overarching mechanisms are summarized and the work is tied together with unifying perspectives.
A New, Facile Synthesis of 4-Alkyl- and Aryl-Substituted 3-Amino-4H-1,2,4-benzothiadiazine 1,1-Dioxides (1986)

A new efficient preparation of 4-alkyl- and aryl-substituted 3-amino- 4H-1,2,4-benzothiadiazine 1,1-dioxides (3) is described via the 3-chloro derivatives of 4-alkyl- and aryl substituted 3-oxo-2H-3,4-dihydro-1,2,4-benzothiadiazine 1,1-dioxides, (1) on their treatment with aqueous ammonia solution.
Daisy-chain gene drives for the alteration of local populations (2016)

Abstract RNA-guided gene drive elements could address many ecological problems by altering the traits of wild organisms, but the likelihood of global spread tremendously complicates ethical development and use. Here we detail a localized form of CRISPR-based gene drive composed of genetic elements arranged in a daisy-chain such that each element drives the next. “Daisy drive” systems can duplicate any effect achievable using an equivalent global drive system, but their capacity to spread is limited by the successive loss of non-driving elements from the base of the chain. Releasing daisy drive organisms constituting a small fraction of the local wild population can drive a useful genetic element to local fixation for a wide range of fitness parameters without resulting in global spread. We additionally report numerous highly active guide RNA sequences sharing minimal homology that may enable evolutionary stable daisy drive as well as global CRISPR-based gene drive. Daisy drives could simplify decision-making and promote ethical use by enabling local communities to decide whether, when, and how to alter local ecosystems. Author’s Summary ‘Global’ gene drive systems based on CRISPR are likely to spread to every population of the target species, hampering safe and ethical use. ‘Daisy drive’ systems offer a way to alter the traits of only local populations in a temporary manner. Because they can exactly duplicate the activity of any global CRISPR-based drive at a local level, daisy drives may enable safe field trials and empower local communities to make decisions concerning their own shared environments. For more details and an animation intended for a general audience, see the summary at Sculpting Evolution .
Fast-switching dual-frequency liquid crystal optical retarder for beam steering applications (2004)

We demonstrate a fast optical modulator capable of switching large amount of optical retardation (a few microns) in less than 1 ms. The result is achieved by employing a dual frequency nematic in cells with high pre-tile alignment and by providing a special addressing scheme that features amplitude and frequency modulated voltage. We explore the effect of time delay and dielectric heating in process of the optical retardation switching.
Autoantibodies against alpha B-crystallin, a candidate autoantigen in multiple sclerosis, are part of a normal human immune repertoire (2006)

Human T-cell responses to the stress protein alpha B-crystallin in multiple sclerosis (MS)-affected brain samples are dominant when compared to other myelin antigens. The establishment of the apparent autoimmune repertoire against this antigen has been suggested to involve cross-priming during viral infection. Yet, another possibility would be that determinant spreading during ocular inflammation could generate a response to alpha B-crystallin, since it is also a major component of the eye. In this study, we compared serum IgG, IgA and IgM repertoires against a range of eye lens-derived ocular antigens using sera from healthy control subjects and MS patients with or without uveitis. This comparison revealed that among ocular antigens, alpha B-crystallin is the dominant target antigen for serum autoantibodies in both MS patients and healthy controls. Uveitis generally did not affect the antibody reactivity profile. These data provide further support for the notion that a normal adult human immune system is selectively reactive to alpha B-crystallin and they indicate that this responsiveness is unlikely to result from determinant spreading following ocular inflammation.
Non-fiducial PPG-based authentication for healthcare application (2017)

Biometrics have a great deal of potential in healthcare applications, most notably authentication for medical record privacy and fraud prevention. In this paper, we examine, for the first time, non-fiducial feature extraction for photo-plethysmography (PPG) based authentication. PPG signals have unique identity properties for human authentication, and are becoming easier to capture by emerging IoT sensors such as MaxFast. Different machine learning techniques are used to compare non-fiducial and fiducial feature extractions. Our experimental results show that 99.84% accuracy with EER of 1.31% can be achieved based on non-fiducial feature extraction.
Options for Development of Environmental Sentinel Biomonitor Systems for Real-Time Detection of Toxic Chemicals in Response to US Military Needs (2002)

Abstract : This report summarizes the substance of a workshop on environmental sentinel biomonitor systems (ESB) held 26-28 Sept. 2001 at the US Army Center for Environmental Health Research. The focus of the workshop was on development and field use of ESB systems as real-time early warning sensors for the presence of toxic industrial chemicals and toxic industrial materials which might present health hazards to exposed personnel. The scope of the workshop was limited to human health considerations and issues, and a number of military scenarios were discussed.
Synthetic Studies of the Flavone Derivatives. V. The Use of DDQ in the Dehydrogenation of Flavanones. (I). (1978)

A novel procedure is described for the expedient preparation of flavone. Application of 2, 3-dichloro-5, 6-dicyano-1, 4-benzoquinone (DDQ) in boiling dioxane as dehydrogenating agent to the several flavanones, e.g. 5, 6, 7-trimethoxy-, 4', 5, 7-trimethoxy-, 4', 5, 6, 7-tetramethoxy-, 4', 5, 6, 7, 8-pentamethoxy-, 4'-benzyloxy-5, 6, 7-trimethoxy-, 3', 4', 5, 6, 7-pentamethoxy-, 4'-benzyloxy-3', 5, 6, 7-tetramethoxy- and 3', 4'-dibenzyloxy-5, 6, 7-trimethoxyflavanone, furnishes a convenient and appropriate method for the preparation of the corresponding flavones, in excellent yields or without any contamination of undesired products.
Data from Up-Regulation of TWIST in Prostate Cancer and Its Implication as a Therapeutic Target (2023)

&lt;div&gt;Abstract&lt;p&gt;Androgen-independent metastatic prostate cancer is the main obstacle in the treatment of this cancer. Unlike a majority of solid cancers, prostate cancer usually shows poor response to chemotherapeutic drugs. In this study, we have shown a potential novel target, TWIST, a highly conserved bHLH transcription factor, in the treatment of prostate cancer. Using malignant and nonmalignant prostate tissues, we found that TWIST expression was highly expressed in the majority (90%) of prostate cancer tissues but only in a small percentage (6.7%) of benign prostate hyperplasia. In addition, the TWIST expression levels were positively correlated with Gleason grading and metastasis, indicating its role in the development and progression of prostate cancer. Furthermore, down-regulation of TWIST through small interfering RNA in androgen-independent prostate cancer cell lines, DU145 and PC3, resulted in increased sensitivity to the anticancer drug taxol-induced cell death which was associated with decreased Bcl/Bax ratio, leading to activation of the apoptosis pathway. More importantly, inactivation of TWIST suppressed migration and invasion abilities of androgen-independent prostate cancer cells, which was correlated with induction of E-cadherin expression as well as morphologic and molecular changes associated with mesenchymal to epithelial transition. These results were further confirmed on the androgen-dependent LNCaP cells ectopically expressing the TWIST protein. Our results have identified TWIST as a critical regulator of prostate cancer cell growth and suggest a potential therapeutic approach to inhibit the growth and metastasis of androgen-independent prostate cancer through inactivation of the &lt;i&gt;TWIST&lt;/i&gt; gene.&lt;/p&gt;&lt;/div&gt;
Investigation of Zigzag Rising Bubble Using Spinning Sphere Model (2006)

A zigzag rising motion is a typical motion of bubbles in bubbly flows. Vortices shedding oscillatory behind a bubble and the wake of the bubble turning periodically similar to a sign of inequality are regarded as the most fundamental causes of the turbulence excited in bubbly flows, because these phenomena produce an alternating lift force on the basis of both conservation laws of circulation and momentum, respectively. In this paper, we discuss the lift production on a zigzag rising bubble trailing a hairpin vortex using a spinning sphere model, which is constructed by us to hold the aerodynamic universality independent of Reynolds number. We fluid-dynamically explicate the alternating lift production on a rising originally nonspin bubble and the zigzag turning of its path, and propose a chain of processes considered in this paper as a fluid-dynamic mechanism that produces the rising bubble zigzag motion.