gebhart/patrepeval huggingface dataset:
Injection Technology (2018)

No AccessInjection Molding Processing DataNov 2018Injection TechnologyAlberto Naranjo C., Maria del Maria del, Juan Diego Sierra M., Juan Rodrigo Sanz, Tim A. OsswaldAlberto Naranjo C., Maria del Maria del, Juan Diego Sierra M., Juan Rodrigo Sanz, Tim A. Osswaldhttps://doi.org/10.3139/9781569906675.002SectionsAboutPDF ToolsAdd to FavoritesDownload CitationTrack CitationsCopy LTI LinkPDF key 'share (en)' returned an object instead of string.FacebookTwitterEmailLinkedIn previous chapternext chapter FiguresReferencesRelatedDetails 2018Pages: 6-13Print ISBN: 978-1-56990-666-8eISBN: 978-1-56990-667-5 Copyright & Permissions© 2019 Carl Hanser Verlag GmbH & Co. KGPDF downloadLoading ...
Fisetin Attenuates Cellular Senescence Accumulation During Culture Expansion of Human Adipose-Derived Stem Cells (2023)

Mesenchymal stem cells (MSCs) have long been viewed as a promising therapeutic for musculoskeletal repair. However, regulatory concerns including tumorgenicity, inconsistencies in preparation techniques, donor-to-donor variability, and the accumulation of senescence during culture expansion have hindered the clinical application of MSCs. Senescence is a driving mechanism for MSC dysfunction with advancing age. Often characterized by increased reactive oxygen species, senescence-associated heterochromatin foci, inflammatory cytokine secretion, and reduced proliferative capacity, senescence directly inhibits MSCs efficacy as a therapeutic for musculoskeletal regeneration. Furthermore, autologous delivery of senescent MSCs can further induce disease and aging progression through the secretion of the senescence-associated secretory phenotype (SASP) and mitigate the regenerative potential of MSCs. To alleviate these issues, the use of senolytic agents to selectively clear senescent cell populations has become popular. However, their benefits to attenuating senescence accumulation in human MSCs during the culture expansion process have not yet been elucidated. To address this, we analyzed markers of senescence during the expansion of human primary adipose-derived stem cells (ADSCs), a population of fat-resident MSCs commonly used in regenerative medicine applications. Next, we used the senolytic agent fisetin to determine if we can reduce these markers of senescence within our culture-expanded ADSC populations. Our results indicate that ADSCs acquire common markers of cellular senescence including increased reactive oxygen species, senescence-associated β-galactosidase, and senescence-associated heterochromatin foci. Furthermore, we found that the senolytic agent fisetin works in a dose-dependent manner and selectively attenuates these markers of senescence while maintaining the differentiation potential of the expanded ADSCs.
Characterizing the short-latency evoked response to intracortical microstimulation across a multi-electrode array (2021)

Abstract Objective Persons with tetraplegia can use brain-machine interfaces to make visually guided reaches with robotic arms. Without somatosensory feedback, these movements will likely be slow and imprecise, like those of persons who retain movement but have lost proprioception. Intracortical microstimulation (ICMS) has promise for providing artificial somatosensory feedback. If ICMS can mimic naturally occurring neural activity, afferent interfaces may be more informative and easier to learn than interfaces that evoke unnaturalistic activity. To develop such biomimetic stimulation patterns, it is important to characterize the responses of neurons to ICMS. Approach Using a Utah multi-electrode array, we recorded activity evoked by single pulses, and short (~0.2 s) and long (~4 s) trains of ICMS at a wide range of amplitudes and frequencies. As the electrical artifact caused by ICMS typically prevents recording for many milliseconds, we deployed a custom rapid-recovery amplifier with nonlinear gain to limit signal saturation on the stimulated electrode. Across all electrodes after stimulation, we removed the remaining slow return to baseline with acausal high-pass filtering of time-reversed recordings. With these techniques, we could record ~0.7 ms after stimulation offset even on the stimulated electrode. Main results We recorded likely transsynaptically-evoked activity as early as ~0.7 ms after single pulses of stimulation that was immediately followed by suppressed neural activity lasting 10–150 ms. Instead of this long-lasting inhibition, neurons increased their firing rates for ~100 ms after trains. During long trains, the evoked response on the stimulated electrode decayed rapidly while the response was maintained on non-stimulated channels. Significance The detailed description of the spatial and temporal response to ICMS can be used to better interpret results from experiments that probe circuit connectivity or function of cortical areas. These results can also contribute to the design of stimulation patterns to improve afferent interfaces for artificial sensory feedback.
Inadvertent and intentional subwavelength surface texture on micro-optical components (2004)

We will discuss the fabrication of several diffractive optical elements (DOEs) for projects at Sandia National Laboratories, which highlight the relative importance of subwavelength surface texture in the componentsi’ performance. This surface texture is in addition to the larger, anisotropic DOE features that manipulate the propagating orders, and is commonly referred to as grass. Surface texture on amorphous or multi-crystalline material is readily apparent in a scanning electron micrograph and is often an unavoidable consequence of the reactive ion etch (RIE) process. Contributing factors are mask erosion, self-masking, and material non-uniformity. In this presentation, we describe and quantify the effects of unavoidable and deliberate surface texture through several projects in progress at Sandia National Laboratories.
Fabrication and electrical characterization of 0.55eV N-on-P InGaAs TPV devices (1999)

Results are presented on the characterization and testing of lattice-mismatched 0.55 eV InGaAs/InP thermophotovoltaic (TPV) cells. A robust cell fabrication technique amenable to high throughput production is presented. A versatile light and dark I-V set up capable of fast screening of the TPV cells and an innovative approach for screening high performance cells are presented. We also report on the effect of lattice-matched InAsP and InAlAs back surface field layers on the performance of the TPV cells.
Bacteriophage-Based Biosensors: A Platform for Detection of Foodborne Bacterial Pathogens from Food and Environment (2022)

Foodborne microorganisms are an important cause of human illness worldwide. Two-thirds of human foodborne diseases are caused by bacterial pathogens throughout the globe, especially in developing nations. Despite enormous developments in conventional foodborne pathogen detection methods, progress is limited by the assay complexity and a prolonged time-to-result. The specificity and sensitivity of assays for live pathogen detection may also depend on the nature of the samples being analyzed and the immunological or molecular reagents used. Bacteriophage-based biosensors offer several benefits, including specificity to their host organism, the detection of only live pathogens, and resistance to extreme environmental factors such as organic solvents, high temperatures, and a wide pH range. Phage-based biosensors are receiving increasing attention owing to their high degree of accuracy, specificity, and reduced assay times. These characteristics, coupled with their abundant supply, make phages a novel bio-recognition molecule in assay development, including biosensors for the detection of foodborne bacterial pathogens to ensure food safety. This review provides comprehensive information about the different types of phage-based biosensor platforms, such as magnetoelastic sensors, quartz crystal microbalance, and electrochemical and surface plasmon resonance for the detection of several foodborne bacterial pathogens from various representative food matrices and environmental samples.
Non-invasive measurement of glucose and pyruvate uptake by individual human oocytes and preimplantation embryos (1989)

Pyruvate and glucose uptake by 73 individual human oocytes and preimplantation embryos was measured non-invasively, using an ultramicrofluorescence assay to analyse changes in substrate levels in microdroplets of culture medium. The uptake of both substrates was measured over successive daily incubations between days 1 (unfertilized oocytes) or 2 ('spare' embryos which were not transferred) and day 6 (day 0 = day of insemination). Under these conditions, 58% (25/43) of fertilized embryos with two pronuclei on day 1 developed to the blastocyst stage by day 6. The pyruvate uptake of these embryos increased from approximately 28 to a maximum of 40 pmol/embryo/h between days 2.5 and 4.5. Similarly, glucose uptake increased from approximately 8 to 14 pmol/embryo/h between days 2.5 and 4.5, but then increased further to 24 pmol/embryo/h on day 5 at the blastocyst stage. [corrected] The pyruvate uptake of fertilized embryos which arrested at cleavage stages was significantly lower than for those which developed to the blastocyst stage. Polyspermic and parthenogenetic embryos, and unfertilized oocytes also had lower pyruvate uptakes at later stages. The glucose uptake of unfertilized oocytes and abnormal embryos never reached the level of fertilized embryos at the blastocyst stage on day 5.5. Non-invasive measurement of pyruvate uptake before embryo transfer may provide a valuable functional criterion for the selection of viable embryos capable of developing to the blastocyst stage.
Effect of Microstructures on Tensile Properties of AZ31 Mg Alloy Processed by ECAP (2005)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.
Peripheral phenotype and gene expression profiles of combined liver–kidney transplant patients (2015)

These data suggest that CLK patients show the immunological influence of both allografts with liver having a greater influence.
Passivity-Based Versus Disturbance Observer Based Robot Control: Equivalence and Stability (1999)

Disturbance observer based robot control algorithms provide good control performance while giving a straightforward method of gain selection. Unfortunately, the stability of these algorithms has not been shown rigorously for nonlinear systems such as robots. This paper demonstrates that a disturbance observer based algorithm can be made equivalent to the well-known passivity-based approaches of Sadegh and Horowitz, and Slotine and Li. This is accomplished by choosing a specific design of the Q(s) filter (described in the paper) of the disturbance observer. By doing so, the stability of the disturbance observer based approach can be proven rigorously. An additional benefit of the equivalence of the two approaches is the ability to combine an adaptive controller to the disturbance observer based approach.
Highly sensitive graphene biosensor by monomolecular self-assembly of receptors on graphene surface (2017)

Graphene has attracted a great deal of interest for applications in bio-sensing devices because of its ultra-thin structure, which enables strong electrostatic coupling with target molecules, and its excellent electrical mobility promising for ultra-fast sensing speeds. However, thickly stacked receptors on the graphene's surface interrupts electrostatic coupling between graphene and charged biomolecules, which can reduce the sensitivity of graphene biosensors. Here, we report a highly sensitive graphene biosensor by the monomolecular self-assembly of designed peptide protein receptors. The graphene channel was non-covalently functionalized using peptide protein receptors via the π-π interaction along the graphene's Bravais lattice, allowing ultra-thin monomolecular self-assembly through the graphene lattice. In thickness dependent characterization, a graphene sensor with a monomolecular receptor (thickness less than 3 nm) showed five times higher sensitivity and three times higher voltage shifts than graphene sensors with thick receptor stacks (thicknesses greater than 20 nm), which is attributed to excellent gate coupling between graphene and streptavidin via an ultrathin receptor insulator. In addition to having a fast-inherent response time (less than 0.6 s) based on fast binding speed between biotin and streptavidin, our graphene biosensor is a promising platform for highly sensitive real-time monitoring of biomolecules with high spatiotemporal resolution.
Dispersion and Stability Optimization of TiO2 Nanoparticles in Cell Culture Media (2010)

Accurate evaluation of engineered nanomaterial toxicity requires not only comprehensive physical-chemical characterization of nanomaterials as produced, but also thorough understanding of nanomaterial properties and behavior under conditions similar to those used for in vitro and in vivo toxicity studies. In this investigation, TiO(2) nanoparticles were selected as a model nanoparticle and bovine serum albumin (BSA) was selected as a model protein for studying the effect of protein-nanoparticle interaction on TiO(2) nanoparticle dispersion in six different mammalian, bacteria, and yeast cell culture media. Great improvement in TiO(2) dispersion was observed upon the addition of BSA, even though the degree of dispersion varied from medium to medium and phosphate concentration in the cell culture media was one of the key factors governing nanoparticle dispersion. Fetal bovine serum (FBS) was an effective dispersing agent for TiO(2) nanoparticles in all six media due to synergistic effects of its multiple protein components, successfully reproduced using a simple "FBS mimic" protein cocktail containing similar concentrations of BSA, γ-globulin, and apo-transferrin.
Measurement of wavefront aberrations of human eyes using a 2-dimensional grating and Fourier analysis (2004)

New methods of measuring aberrations of human eyes, using the Talbot effect of a 2-dimensional grating as a wavefront sensor and Fourier transform for calculating tilt of the wavefront, are shown. Using these methods, wavefront aberrations can be measured even when the obtained image is degraded by the scattering.
TFTR DT experiments (1997)

The Tokamak Fusion Test Reactor (TFTR) is a large tokamak which has performed experiments with 50:50 deuterium - tritium fuelled plasmas. Since 1993, TFTR has produced about 1090 D - T plasmas using about 100 grams of tritium and producing about 1.6 GJ of D - T fusion energy. These plasmas have significant populations of 3.5 MeV alphas (the charged D - T fusion product). TFTR research has focused on alpha particle confinement, alpha driven modes, and alpha heating studies. Maximum D - T fusion power production has aided these studies, requiring simultaneously operation at high input heating power and large energy confinement time (to produce the highest temperature and density), while maintaining low impurity content. The principal limitation to the TFTR fusion power production was the disruptive stability limit. Secondary limitations were the confinement time, and limiter power handling capability.
I3.2: Invited Paper: A Multiview 3D Holochat System (2015)

We present a lightweight holographic video chat system relying on a cheap camera array to stream full parallax 64‐view 3D content live onto a mobile holographic display by LEIA Inc. The system auto‐calibrates the camera views to a simple target and is suitable for real‐time communication over a peer‐to‐peer network. The zoom level and focal (zero‐disparity) plane location of the system can be adjusted in software and the depth of field can be adjusted by simple modification of the camera baseline.
Comparison of MR imaging with PET and ictal SPECT in 118 patients with intractable epilepsy. (1999)

Overall concordance among the techniques is approximately two thirds or less in lateralizing epileptogenic foci. PET is the most sensitive, even though it provides a broad approximate nature of the epileptogenic zone, which is not adequate for precise surgical localization of epilepsy. PET and/or ictal SPECT may be used as complementary tools in cases of inconclusive lateralization with ictal video/EEG and MR imaging.
ChemInform Abstract: Non‐Destructive Removal of the Bornanesultam Auxiliary in α‐ Substituted N‐Acylbornane‐10,2‐sultams under Mild Conditions: An Efficient Synthesis of Enantiomerically Pure Ketones and Aldehydes. (1997)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Localization of Agonist- and Antagonist-Binding Domains of Human Corticotropin-Releasing Factor Receptors (1997)

The CRF receptors, CRFR1 and CRFR2, are members of the G protein-coupled receptor superfamily. Despite their considerable sequence similarity, CRFR1 and CRFR2 have quite different affinities for the peptide ligand rat/human CRF. Previous studies using chimeric receptors between human CRFR1 and CRFR2 have identified three potentially important regions in the second and third extracellular domains of CRF receptor for the binding of rat/human CRF. The present report further demonstrates that these same three regions also affect the binding of urocortin and sauvagine, two other members of the CRF peptide family, albeit to different extents. We also show that a fourth region in the third extracellular domain, Asp254, has been identified to be important for sauvagine but not CRF or urocortin binding. Thus, the three peptide ligands not only interact with a different set of regions on CRFR1 and CRFR2 but also differentially interact with some of the same regions. These data could, at least in part, account for the much higher affinity of CRFR2 for urocortin and sauvagine compared with rat/human CRF. We have also identified two amino acid residues, His199 in the third transmembrane domain and Met276 in the fifth transmembrane domain, that are important for binding the non-peptide high-affinity CRFR1 antagonist NBI 27914. Mutations of His199 and Met276 to the corresponding amino acids in CRFR2 each decreased the binding affinity of NBI 27914 for CRFR1 by 40- and 200-fold, respectively. This suggests that the transmembrane regions are critically important in forming the binding pocket for the nonpeptide antagonist.
Distributed recursive composite hypothesis testing: Imperfect communication (2016)

This paper focuses on the problem of distributed composite hypothesis testing in a noisy network of sparsely interconnected agents in which a pair of agents exchange information over an additive noise channel. The network objective is to test a simple null hypothesis against a composite alternative concerning the state of the field, modeled as a vector of (continuous) unknown parameters determining the parametric family of probability measures induced on the agents' observation spaces under the hypotheses. A recursive generalized likelihood ratio test (GLRT) type algorithm in a distributed setup of the consensus+innovations form is proposed, in which the agents update their parameter estimates and decision statistics by simultaneously processing the latest sensed information (innovations) and information obtained from neighboring agents (consensus). This paper characterizes the conditions and the testing algorithm design parameters which ensure that the probabilities of decision errors decay to zero asymptotically in the large sample limit.
Identifying and Validating Material Models with Non-Unique Parameter Sets. (2016)

Abstract not provided.
Influence of illumination tilt on imaging (2005)

Tilt in the optic axis of the illumination system with respect to the projection lens manifests itself as a shift of the illumination source intensity distribution in the pupil plane of that projection lens. The impact of this error category upon various types of patterns and among various imaging configurations is studied through image simulation as well as experiment. Issues addressed will include:-Methods of measuring illuminator tilt -Sensitivity of sample cases to illumination tilt, including pattern placement issues as well as pattern fidelity issues -Aberration influence and coupling with illumination tilt influences -Ramifications of illuminator tilt impacts. Finally, comments will be made regarding this error category as it may impact the increasingly stringent process complexities and reducing linewidth sizes required by the processes being developed for the near future.
Hydrocarbon Recovery Maximization in Reservoirs Located Close to Potential Water Producer Horizons: New Proposed Methodology to Combine Water Control and Fracture Stimulation Processes (2015)

Abstract Hydrocarbon production and recovery maximization can be a challenging task in some specific areas or reservoirs. Specifically, in low permeability environments, hydraulic fracturing is a necessary step to improve the wells productivity and it can be easily justified or applied in reservoirs where a nearby water bearing zone is not present or the water bearing zone is far away from the productive horizons. When bottom water or water bearing zones are located near the oil producer layers, hydraulic fracture stimulation is normally aborted or the potential layer is abandoned or not completed; unfortunately, the luxury of stimulation or completion abortion is not really the best decision because a large amount of hydrocarbons is located in such complex environments. For the situations where the fracture stimulation could not be avoided, the industry has used different alternatives to minimize water production. These include selective perforation and controlled fracture stimulation, selective water control treatment into the water producer layer followed by fracturing, or combining the water control and fracture stimulation in one. For the latter methodology, the water control chemical is squeezed first into the fractured interval or the chemical is added into the fracturing fluid pad to minimize water mobility. However, this methodology relies on the compatibility of the fracturing fluid and the water control chemicals or the migration of the water control chemical from the fracturing fluid system to the water source layers to be effective. This paper proposes a new methodology to minimize water intrusion in cases where fracture stimulation is necessary in areas with bottom water or water bearing zones near the oil producer layers by performing on-the-fly coating of proppant with a relative permeability modifier (RPM) chemical. In the new proposed methodology, water mobility in the water producer layer will be controlled by the proppant bed coated by the RPM.
The metabolic demands of cancer cells are coupled to their size and protein synthesis rates (2013)

The NCI60 cell lines display various metabolic activities, and the type of metabolic activity that they possess correlates with their cell volume and protein content. In addition to cell proliferation, cell volume and/or biomarkers of protein synthesis may predict response to drugs targeting cancer metabolism.