gebhart/patrepeval huggingface dataset:
Phosphorylation potential of Drosophila E-Cadherin intracellular domain is essential for development and adherens junction biosynthetic dynamics regulation (2017)

Phosphorylation of a highly conserved serine cluster in the intracellular domain of E-Cadherin is essential for binding to β-Catenin <i>in vitro</i> In cultured cells, phosphorylation of specific serine residues within the cluster is also required for regulation of adherens junction (AJ) stability and dynamics. However, much less is known about how such phosphorylation of E-Cadherin regulates AJ formation and dynamics <i>in vivo</i> In this report, we generated an extensive array of <i>Drosophila</i> E-Cadherin (DE-Cad) endogenous knock-in alleles that carry mutations targeting this highly conserved serine cluster. Analyses of these mutations suggest that the overall phosphorylation potential, rather than the potential site-specific phosphorylation, of the serine cluster enhances the recruitment of β-Catenin by DE-Cad <i>in vivo</i> Moreover, phosphorylation potential of the serine cluster only moderately increases the level of β-Catenin in AJs and is in fact dispensable for AJ formation <i>in vivo</i> Nonetheless, phosphorylation-dependent recruitment of β-Catenin is essential for development, probably by enhancing the interactions between DE-Cad and α-Catenin. In addition, several phospho-mutations dramatically reduced the biosynthetic turnover rate of DE-Cad during apical-basal polarization, and such biosynthetically stable DE-Cad mutants specifically rescued the polarity defects in embryonic epithelia lacking the polarity proteins Stardust and Crumbs.
Conventionalization of grammatical anomolies through linearization (2018)

This article explores the diachronic influence of linear form in the grammaticalization of new constructions, especially those with distinctive functional and structural properties. For example, it is shown that the coordination of prenominal adjectives without an overt conjunction in English (relevant, interesting research) is due to reanalysis of an original historical structure expressing a modification relationship (tall young man). Examples of this type are discussed in the context of grammaticality illusions, where speakers parse structures and meanings other than intended by the speaker or when the blending of two grammatical constructions produces a syntactic amalgam. Diachronic changes based on linear form thus enter the grammar as exceptional but conventionalized constructions. The paper emphasizes the need to consider surface form as well as structure and meaning in both diachronic and synchronic analysis.
A QSPR Investigation of Thermal Stability of [Al(CH3)O]n Oligomers in Methylaluminoxane Solution: The Identification of a Geometry-Based Descriptor (2012)

Fifty-six methylaluminoxane (MAO) cage structures with the general formula [AlMeO]n, where n ranges from 6 to 12, have been optimized using density functional theory calculations in order to identify relevant chemical descriptors to reveal the thermodynamic stability of MAO. First, NMR properties were calculated for the most stable optimized structures, showing a good agreement with experimental results and revealing a relationship between the calculated 27Al NMR shifts and local geometry of the aluminum atoms. Then, different electronic and geometric descriptors of optimized structures have been calculated and compared via a QSPR approach to various thermodynamic functions: internal energy, enthalpy, and Gibbs free enthalpy (ΔGr), leading to the identification of a relevant descriptor based on the calculation of the distortion of aluminum sites in the [AlMeO]n structures. The identified descriptor was thus applied to predict ΔGr for [AlMeO]n structures with n ranging from 6 to 33. The study of the evolution of ΔGr as a function of temperature and size (n) reveals that there is a window of stable sizes for [AlMO]n depending on the temperature, which is between n = 12 and n = 24. Low temperatures disfavors smaller (n < 12) sized oligomers due to strong distortion of aluminum sites, while at high temperatures [AlMO]n structures with n greater than 18 become destabilized due to entropic effects.
ChemInform Abstract: An Improved Method of Amide Synthesis Using Acyl Chlorides. (2009)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Image Intensification for Low-Light Face Recognition (2006)

This paper presents the first published systematic study of face recognition performance as a function of light level using intensified near infrared imagery. This technology is the most prevalent in both civilian and military night vision equipment, and provides enough intensification for human operators to perform standard tasks under extremely low-light conditions. We describe a comprehensive data collection effort undertaken by the authors to image subjects under carefully controlled illumination and quantify the performance of standard face recognition algorithms on visible and intensified imagery as a function of light level. Performance comparisons for automatic face recognition are reported using the standardized implementations from the CSU Face Identification Evaluation System. The results contained in this paper should constitute the initial step for analysis and deployment of face recognition systems designed to work in low-light level condi
Structure of the Integrin β3 Transmembrane Segment in Phospholipid Bicelles and Detergent Micelles (2008)

Integrin adhesion receptors transduce bidirectional signals across the plasma membrane, with the integrin transmembrane domains acting as conduits in this process. Here, we report the first high-resolution structure of an integrin transmembrane domain. To assess the influence of the membrane model system, structure determinations of the β3 integrin transmembrane segment and flanking sequences were carried out in both phospholipid bicelles and detergent micelles. In bicelles, a 30-residue linear α-helix, encompassing residues I693−H772, is adopted, of which I693-I721 appear embedded in the hydrophobic bicelle core. This relatively long transmembrane helix implies a pronounced helix tilt within a typical lipid bilayer, which facilitates the snorkeling of K716’s charged side chain out of the lipid core while simultaneously immersing hydrophobic L717−I721 in the membrane. A shortening of bicelle lipid hydrocarbon tails does not lead to the transfer of L717−I721 into the aqueous phase, suggesting that the reported embedding represents the preferred β3 state. The nature of the lipid headgroup affected only the intracellular part of the transmembrane helix, indicating that an asymmetric lipid distribution is not required for studying the β3 transmembrane segment. In the micelle, residues L717−I721 are also embedded but deviate from linear α-helical conformation in contrast to I693−K716, which closely resemble the bicelle structure.
Preparation of ABS (acrylonitrile/butadiene/styrene) latexes using hydroperoxide redox initiators (1990)

Abstract The 80/20 (w/w) azeotropic styrene/acrylonitrile mixture was copolymerized at 35, 50, and 70°C in crosslinked polybutadiene seed latexes of varying particle size (range 113–242 nm) using the t ‐butyl or cumene hydroperoxide/sodium formaldehyde sulfoxylate dihydrate/EDTA‐chelated Fe +2 redox initiator. Other initiators used for comparison were: (1) ammonium persulfate at 70°C; (2) ammonium persulfate/sodium bisulfite redox initiator at 35 and 50°C; (3) oil‐soluble, 2,2'‐azobis(2,4‐dimethylvaleronitrile) at 50°C. The following polymerization parameters were varied systematically: (1) polybutadiene seed latex particle size; (2) monomer/polymer swelling ratio; (3) polymerization temperature; (4) type and concentration of initiator; and (5) mode (and duration) of initiator addition. The following parameters were determined: (1) polymer yield (final conversion); (2) variation of conversion with time; (3) morphology of the latex particles; (4) amount of coagulum; and (5) fraction of styrene/acrylonitrile monomer mixture grafted to the polybutadiene seed latex (defined here as grafting efficiency). For polymerizations using the t ‐butyl or cumene hydroperoxide/sodium formaldehyde sulfoxylate dihydrate/EDTA‐chelated Fe +2 redox initiator, the rates of polymerization were faster, the grafting efficiencies were greater, and the amounts of coagulum were greater at 70°C than at 50 or 35°C. The rates of polymerization were decreased by decreasing the concentration of redox initiator or adding it incrementally. A 1.00/1.00/0.08 (w/w) t ‐butyl or cumene hydroperoxide/sodium formaldehyde sulfoxylate dihydrate/EDTA‐chelated Fe +2 ratio gave reasonable polymerization rates at 35 and 50°C, and a 0.48/1.00/0.07 ratio, at 70°C. The latexes were stabilized during polymerization with 3% (based on seed solids) nonionic Igepal CO‐990 at 35°C, and 6% at 50 and 70°C. The grafting efficiency increased with decreasing particle size of the polybutadiene seed latex and decreasing monomer/polymer ratio. The rate of polymerization increased with decreasing monomer/polymer ratio. The monomer/polymer ratio also affected the morphology of the particles; higher values gave inclusions of poly(styrene‐ co ‐acrylonitrile) in the polybutadiene particles. There were no significant differences in polymerization kinetics or grafting efficiency between the t ‐butyl and cumene hydroperoxides.
Optical thin film structures for dispersion compensation in femtosecond laser systems (1993)

Pulse duration of recent mode-locked femtosecond pulse solid state laser systems is limited by the third-order dispersion originating from prism diffraction, prism material, the laser active material and other cavity elements. The frequency-dependent group delay introduced by specially designed thin film structures can be effectively utilized to minimize the resultant intracavity third- and higher-order dispersion.
4121 The beneficial, anti-fibrotic effects of chemokine receptor 2 and 5 antagonists on fat-exposed mouse primary hepatic stellate cells (pHSCs) (2020)

OBJECTIVES/GOALS: Non-alcoholic steatohepatitis (NASH) is a leading cause of cirrhosis in the world for which no anti-fibrotic therapies exist. We hypothesized that BMS-22 and maraviroc (MVC), chemokine receptor 2 (CCR2) and 5 (CCR5) antagonists, respectively, would diminish the fibrogenic activity of "fat-exposed" murine pHSCs. METHODS/STUDY POPULATION: pHSCs were isolated from livers of 6 week old male mice following 4 weeks on a NASH-inducing choline-deficient high fat diet (CDAHFD, “fat-exposed”) or standard diet (SD) and passaged in vitro . Early passage (6-12) pHSCs were plate-adhered and TGF-b-treated (10ng/mL) to maximally activate their pro-fibrogenic genes, collagen 1α1 ( Col1A1), tissue inhibitor of metalloproteinase 1 ( TIMP1) , or α-smooth muscle actin (ACTA2 ). CDAHFD and SD pHSCs were then treated for 48 hours with increasing doses of BMS-22 or MVC (range: 0.3-120ng/mL) to determine (1) the degree of attenuation of the pro-fibrogenic response as measured by qPCR of fibrogenic genes ( Col1A1, TIMP1,ACTA2); (2) enhancement of a fibrolytic response as measured by qPCR of matrix metalloproteinases (MMP ) 2, 9 and 13 genes; and (3) pHSC migration using the scratch assay. Cell viability and CCR2 and CCR5 gene expression in response to escalating doses of antagonists were also measured. RESULTS/ANTICIPATED RESULTS: Plate- and TGF-b activated CDAHFD pHSCs had a 2-fold greater, dose-dependent attenuation of their pro-fibrogenic activity in response to BMS-CCR2-22 and MVC, when compared with plate- and TGF-b activated SD pHSCs, as measured by reductions in collagen 1α1 ( Col1A1 ) and α-smooth muscle actin ( ACTA2 ) gene expression. TIMP1 gene expression was unaffected by drug treatment for 48 hours. Cell viability was not affected up to doses of 30ng/mL of each drug. pHSCs also demonstrated a dose-dependent increase in CCR2, CCR5 and MMP-9 gene expression in response to surface receptor antagonism. Migration assays comparing CDAHFD and SD pHSCs in response to escalating doses of MVC and BMS-22 are ongoing and expected to demonstrate a significantly decreased migratory capacity of CDAHFD pHSCs than SD pHSCs in response to therapy, reflecting the increased susceptibility of the “fat-exposed” pHSCs to anti-fibrotic therapy than normal pHSCs. DISCUSSION/SIGNIFICANCE OF IMPACT: Anti-fibrotic drugs that dampen pro-fibrogenic activities of “fat-exposed” pHSCs are urgently needed. CCR2 and CCR5 antagonists, BMS-22 and MVC, respectively, can selectively dampen the pro-fibrogenic response of fat-exposed pHSCs, and must be considered for future trials in human NASH. CONFLICT OF INTEREST DESCRIPTION: Dr. Jill Smith has a patent licensing agreement with Immune Therapeutics, Inc.
In Situ Modification of Metal–Organic Frameworks in Mixed‐Matrix Membranes (2015)

Abstract Processable films of metal–organic frameworks (MOFs) have been long sought to advance the application of MOFs in various technologies from separations to catalysis. Herein, MOF–polymer mixed‐matrix membranes (MMMs) are described, formed on several substrates using a wide variety of MOF materials. These MMMs can be delaminated from their substrates to create free‐standing MMMs that are mechanically stable and pliable. The MOFs in these MMMs remain highly crystalline, porous, and accessible for further chemical modification through postsynthetic modification (PSM) and postsynthetic exchange (PSE) processes. Overall, the findings here demonstrate a versatile approach to preparing stable functional MMMs that should contribute significantly to the advancement of these materials.
Characterization of cationic rhodium isocyanide oligomers in aqueous solutions (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCharacterization of cationic rhodium isocyanide oligomers in aqueous solutionsI. S. Sigal and Harry B. GrayCite this: J. Am. Chem. Soc. 1981, 103, 9, 2220–2225Publication Date (Print):May 1, 1981Publication History Published online1 May 2002Published inissue 1 May 1981https://pubs.acs.org/doi/10.1021/ja00399a014https://doi.org/10.1021/ja00399a014research-articleACS PublicationsRequest reuse permissionsArticle Views142Altmetric-Citations34LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
TRANSIENT RENAL EFFECTS OF SILDENAFIL IN MALE KIDNEY TRANSPLANT RECIPIENTS1 (2001)

The oral administration of sildenafil in KTRs did not impair the function of the graft. In terms of renal physiology, the observed modifications did not warrant any specific precautions when offering sildenafil to KTRs suffering from erectile dysfunction.
Superior survival associated with transplantation of matched unrelated versus one-antigen–mismatched unrelated or highly human leukocyte antigen– disparate haploidentical family donor marrow grafts for the treatment of hematologic malignancies: establishing a treatment algorithm for recipients of alternative donor grafts (2002)

The purpose of this study was to compare transplantation outcomes in patients with hematologic malignancies who received marrow grafts from either phenotypically matched unrelated, one-antigen-mismatched unrelated, or highly human leukocyte antigen (HLA)-disparate family donors. Between 1993 and 2000, 139 patients underwent transplantation from unrelated donors (81 matched and 58 mismatched) and 48 patients received marrow grafts from family donors that were mismatched at 2, 3, or 4 of 8 HLA loci. All patients received a standardized conditioning regimen and a graft-versus-host disease (GVHD) prophylaxis schedule with the exception of recipients of haploidentical marrow grafts, who received antithymocyte globulin after bone marrow transplantation as additional immunosuppression. There was no statistically significant difference in the rate of engraftment, or the cumulative incidences of acute and chronic GVHD between any of the 3 groups. The 2-year cumulative incidence of relapse was lower in matched unrelated patients (25%, P =.01) and mismatched unrelated patients (26%, P =.014) than in haploidentical patients (42%). Transplant-related mortality was significantly higher in recipients of mismatched unrelated grafts (45%, P =.01) and haploidentical grafts (42%, P =.001) compared with recipients of matched unrelated marrow grafts (23%). This resulted in a significantly higher probability of overall survival for matched unrelated patients (58%) versus either mismatched unrelated (34%, P =.01) or haploidentical (21%, P =.002) patients. There was no statistically significant difference in survival between patients who received mismatched unrelated grafts versus those who received haploidentical grafts. This study supports a donor selection algorithm whereby patients who lack a closely matched family donor be offered a phenotypically matched unrelated donor if available. There is no apparent advantage to using a mismatched unrelated versus a highly HLA-disparate family donor.
Two modes of surface roughening during plasma etching of silicon: Role of ionized etch products (2014)

Atomic- or nanometer-scale surface roughening has been investigated during Si etching in inductively coupled Cl2 plasmas, as a function of rf bias power or ion incident energy Ei, by varying feed gas flow rate, wafer stage temperature, and etching time. The experiments revealed two modes of surface roughening which occur depending on Ei: one is the roughening mode at low Ei &amp;lt; 200–300 eV, where the root-mean-square (rms) roughness of etched surfaces increases with increasing Ei, exhibiting an almost linear increase with time during etching (t &amp;lt; 20 min). The other is the smoothing mode at higher Ei, where the rms surface roughness decreases substantially with Ei down to a low level &amp;lt; 0.4 nm, exhibiting a quasi-steady state after some increase at the initial stage (t &amp;lt; 1 min). Correspondingly, two different behaviors depending on Ei were also observed in the etch rate versus Ei curve, and in the evolution of the power spectral density distribution of surfaces. Such changes from the roughening to smoothing modes with increasing Ei were found to correspond to changes in the predominant ion flux from feed gas ions Clx+ to ionized etch products SiClx+ caused by the increased etch rates at increased Ei, in view of the results of several plasma diagnostics. Possible mechanisms for the formation and evolution of surface roughness during plasma etching are discussed with the help of Monte Carlo simulations of the surface feature evolution and classical molecular dynamics simulations of etch fundamentals, including stochastic roughening and effects of ion reflection and etch inhibitors.
Synthesis and analysis of novel polymers with high permselectivity and permeability in gas separation applications. Progress report, December 1990--November 1991 (1991)

We have synthesized and completed characterization of permeability and selectivity properties of a group of polysulfones and polyether ketones with the potential for higher use temperatures, as well as members of a series of polyesters derived from spirobiindane bisphenol monomer in conjunction with meta and para substituted diacid chlorides. We have also synthesized and characterized the gas transport and thermal properties of diphenyl substituted polyphenylene oxide. The diphenyl substituted material has a potential for higher temperature applications than the standard dimethyl substituted polymer. The temperature dependence of the gas transport properties for the oxygen/nitrogen system was characterized over the range from 35 to 65{degree}C for both of these analog materials.
A smart triggering scheme to reduce service interruption during heterogeneous handovers (2008)

Multi-radio devices provide end-users the ability to achieve ubiquitous and seamless connectivity anytime, anywhere across heterogeneous networks. Minimal service disruption while a device roams across networks is key for a successful deployment. Todaypsilas roaming decisions are reactive and traditionally based on thresholds for signal strength or other radio properties, leading to undesirable handover delays. This paper introduces an Intel/BT client architecture to support seamless mobility for multi-radio devices and proposes a novel approach to predict when a device needs to take proactive actions to perform handovers. Significant improvement in reducing service discontinuity time is demonstrated by applying our approach to WiFi and WiMax networks. We also validate the architecture and algorithm benefits using our WiFi/WiMax multi-radio prototype in heterogeneous wireless network environments.
P3‐053: ATP‐binding cassette transporter A7 (ABCA7) effects on amyloid processing and relevance to Alzheimer's disease (2012)

ATP-binding cassette transporter A7 (ABCA7) is a member of the ABC transporter family associated with phagocytosis. ABCA7 is highly homologous to ABCA1, which act as critical gatekeeper regulating cellular cholesterol efflux to extracellular apolipoprotein acceptors. Transcriptional regulation of ABCA7 is negatively regulated by cell cholesterol, mediated by sterol regulatory element binding protein 2, in the opposite direction to that of ABCA1. Genetic, biochemical and pharmacological evidences indicate that alternations in cholesterol homeostasis increase the risk of Alzheimer's disease (AD). ABCA7 was recently identified as the susceptibility loci by a genome-wide association study (GWAS) which revealed specific AD risk alleles. However, molecular mechanism of ABCA7 pathway under the AD is still unclear. We have therefore undertaken investigations to determine ABCA7 effects on amyloid precursor protein (APP) processing, Aß production and Aß uptake in vitro and/or in vivo. Over-expression of full-length human ABCA7 in HeLa cells was associated with lower Aß production while ABCA7 knockdown resulted in modestly higher Aß production when co-transfected with APP. Similar events appear to occur in vivo shown by the examination of endogenous amyloid processing in an ABCA7 knock-out mouse model. Endogenous murine Aβ expression levels on the ABCA7 ablated background were higher than that in wild-type control mice at early ages. However, this effect was reduced as the mice aged suggesting a compensatory mechanism for APP processing following the loss of ABCA7. The influence of high cholesterol was also investigated, and endogenous murine Aß levels in the ABCA7 knockout mice were significantly elevated in mice on high-fat diets. Wild-type mice on a similar diet also displayed elevated Aß but these were slightly lower than those observed in the ABCA7 knockouts. These results imply that cholesterol affects on Aß processing through not only γ-secretase pathway but also Aß-secretase or potentially APP expression and/or turnover. Taken together, ABCA7 seems to be one of the key molecules linking not only sterol homeostasis but also Alzheimer's disease potentially through APP processing and Aß production.