gebhart/patrepeval huggingface dataset:
New Highly Stereoselective Synthesis of (Z)-4-Hydroxytamoxifen and (Z)-4-Hydroxytoremifene via McMurry Reaction (1996)

ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTNew Highly Stereoselective Synthesis of (Z)-4-Hydroxytamoxifen and (Z)-4-Hydroxytoremifene via McMurry ReactionSylvain Gauthier, Josée Mailhot, and Fernand LabrieView Author Information Medicinal Chemistry Division, Laboratory of Molecular Endocrinology, CHUL Research Centre, Québec City, Québec G1V 4G2, Canada Cite this: J. Org. Chem. 1996, 61, 11, 3890–3893Publication Date (Web):May 31, 1996Publication History Received28 December 1995Published online31 May 1996Published inissue 1 January 1996https://pubs.acs.org/doi/10.1021/jo952279lhttps://doi.org/10.1021/jo952279lbrief-reportACS PublicationsCopyright © 1996 American Chemical SocietyRequest reuse permissionsArticle Views2046Altmetric-Citations63LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Aromatic compounds,Chemical synthesis,Hydrocarbons,Molecular structure,Stereoselectivity Get e-Alerts
Single ion implantation for solid state quantum computer \ndevelopment (2001)

Several solid state quantum computer schemes are based on the manipulation of electron and/or nuclear spins of single 31P atoms in a solid matrix. The fabrication of qubit arrays requires the placement of individual atoms with nanometer precision and high efficiency. We describe the status of our development of a low energy, single ion implantation scheme for 31Pq+ ions. High ion charge states enable registration of single ion impacts with unity efficiency through the detection of secondary electrons. Imaging contrast in secondary electron emission allows alignment of the implantation and integration with consecutive lithography steps. Critical issues of process integration and resolution limiting factors are discussed.
Heterochronic parabiosis reprograms the mouse brain transcriptome by shifting aging signatures in multiple cell types (2022)

Abstract Aging is a complex process involving transcriptomic changes associated with deterioration across multiple tissues and organs, including the brain. Recent studies using heterochronic parabiosis have shown that various aspects of aging-associated decline are modifiable or even reversible. To better understand how this occurs, we performed single-cell transcriptomic profiling of young and old mouse brains following parabiosis. For each cell type, we catalogued alterations in gene expression, molecular pathways, transcriptional networks, ligand-receptor interactions, and senescence status. Our analyses identified gene signatures demonstrating that heterochronic parabiosis regulates several hallmarks of aging in a cell-type-specific manner. Brain endothelial cells were found to be especially malleable to this intervention, exhibiting dynamic transcriptional changes that affect vascular structure and function. These findings suggest novel strategies for slowing deterioration and driving regeneration in the aging brain through approaches that do not rely on disease-specific mechanisms or actions of individual circulating factors.
Midinfrared frequency combs from coherent supercontinuum in chalcogenide and optical parametric oscillation (2014)

We observe the coherence of the supercontinuum generated in a nanospike chalcogenide-silica hybrid waveguide pumped at 2 μm. The supercontinuum is shown to be coherent with the pump by interfering it with a doubly resonant optical parametric oscillator (OPO) that is itself coherent with the shared pump laser. This enables coherent locking of the OPO to the optically referenced pump frequency comb, resulting in a composite frequency comb with wavelengths from 1 to 6 μm.
Bacterial Protease Lon Is a Site-specific DNA-binding Protein (1997)

The product of the Escherichia coli lon gene is the ATP-dependent Lon protease. Lon contributes to the regulation of several important cellular functions, including radiation resistance, cell division, filamentation, capsular polysaccharide production, lysogeny of certain bacteriophages, and proteolytic degradation of certain regulatory and abnormal proteins. Lon homologues are also found in several widely divergent bacteria, as well as in the mitochondria of yeast and humans. E. coli Lon has long been known to bind to DNA, but this interaction has not been further characterized and has generally been assumed to be nonspecific. We now demonstrate that E. coli Lon can bind to a TG-rich DNA promoter element in a sequence-specific manner. This finding is based on the results of experiments employing SouthWestern blotting, protein purification, "shift-shift" electrophoretic mobility shift assays, electrophoretic mobility shift assays using in vitro transcribed and translated Lon, and DNase footprinting. Site-specific DNA binding is likely to be an additional important biochemical characteristic of the multifaceted Lon protease.
Resampling‐Based Empirical Bayes Multiple Testing Procedures for Controlling Generalized Tail Probability and Expected Value Error Rates: Focus on the False Discovery Rate and Simulation Study (2008)

This article proposes resampling-based empirical Bayes multiple testing procedures for controlling a broad class of Type I error rates, defined as generalized tail probability (gTP) error rates, gTP (q,g) = Pr(g (V(n),S(n)) > q), and generalized expected value (gEV) error rates, gEV (g) = E [g (V(n),S(n))], for arbitrary functions g (V(n),S(n)) of the numbers of false positives V(n) and true positives S(n). Of particular interest are error rates based on the proportion g (V(n),S(n)) = V(n) /(V(n) + S(n)) of Type I errors among the rejected hypotheses, such as the false discovery rate (FDR), FDR = E [V(n) /(V(n) + S(n))]. The proposed procedures offer several advantages over existing methods. They provide Type I error control for general data generating distributions, with arbitrary dependence structures among variables. Gains in power are achieved by deriving rejection regions based on guessed sets of true null hypotheses and null test statistics randomly sampled from joint distributions that account for the dependence structure of the data. The Type I error and power properties of an FDR-controlling version of the resampling-based empirical Bayes approach are investigated and compared to those of widely-used FDR-controlling linear step-up procedures in a simulation study. The Type I error and power trade-off achieved by the empirical Bayes procedures under a variety of testing scenarios allows this approach to be competitive with or outperform the Storey and Tibshirani (2003) linear step-up procedure, as an alternative to the classical Benjamini and Hochberg (1995) procedure.
Electrochemical evidences of morphological transformation in ordered mesoporous titanium oxide thin films (2005)

We provide the first electrochemical evidence of permeability changes in continuous mesoporous TiO2 thin film electrodes induced by nanocrystallisation.
Trust Management Tools (2011)

After entering the realm of computing over a decade ago, the term trust management has evolved to encompass many concepts and mechanisms that bring it closer to its intuitive interpretation. This chapter will outline the evolution and present the catalyst(s) for each phase in its metamorphosis. In each stage, trust management tools were constructed to showcase the current understanding in the field. These tools will be discussed and their strengths, domains of application, and scope for improvement presented. The foundation of trust management technology can be found in security notions, for example credentials, in mathematical computation and in formal (and informal) reputation models. Each of these categories and their hybrids will be highlighted. This chapter should leave the reader with (1) a holistic view of the trust management problem, (2) a clear differentiation of trust management from other fields (and terms) in the computer security arena, and (3) knowledge of the appropriate domain of usage for each system.
Enhanced IgG Hexamerization Mediates Efficient C1q Docking and Complement-Dependent Cytotoxicity; Preclinical Proof Of Concept On Primary CLL and Burkitt Lymphoma (2013)

Abstract Introduction We recently discovered that complement is activated by IgG hexamers that assemble on the surface of cells following antigen binding. The formation of hexamers is dependent on intermolecular interactions in the Fc interface between neighboring IgG molecules. This seminal finding allowed us to identify mutations that enhance the ability of IgG antibodies to bind C1 and induce complement-dependent lysis. Objectives To study the role of Fc-mediated intermolecular contacts in complement-dependent cytotoxicity (CDC) of cancer cells by therapeutic IgG antibodies in biological relevant in vitro and in vivo model systems and to exploit this mechanism to improve CDC activity. M&amp;M Based on an IgG hexamer crystal structure, we generated Fc mutations that were predicted to influence Fc:Fc interactions between IgG antibodies and we screened for improved C’ activation. The effect of these mutations on in vitro C1q binding and CDC-mediated killing of tumor B cell lines and patient-derived chronic lymphatic leukemia (CLL) cells was studied with therapeutic antibodies targeting different B cell antigens. The effect of a CDC-enhancing mutation was further studied in a Burkitt lymphoma xenograft model. Results Here we show that the assembly of IgG hexamers on the cell surface represents an important step for efficient complement activation via the classical pathway by therapeutic antibodies against hematological targets. Critical amino acid residues in the Fc:Fc contacts between IgG molecules were identified and individual mutants with increased complement activation generated. Interestingly, introduction of an enhancing mutation was shown to significantly increase complement activation not only of antibodies capable of inducing CDC, but also of antibodies that were devoid of such activity. We provide a proof-of-concept for CD19, Type I and Type II CD20, CD38 and CD52 antibodies. Importantly, we were able to achieve or enable significant CDC of a panel of primary CLL cells by use of this novel approach. Finally, we show that the enhancing mutation resulted in a significant increase in the inhibition of in vivo tumor growth by a CD20 antibody in a subcutaneous Raji xenograft model in SCID mice. Conclusion The data obtained provide a novel insight into the mechanism of complement-dependent lysis. This mechanism is shown to be relevant for a panel of therapeutic antibodies against hematological targets. Mutations that improved the formation of Fc:Fc-mediated hexamerization resulted in strongly increased CDC of complement-resistant primary CLL cells and improved efficacy in an in vivo model with a Burkitt lymphoma cell line. Disclosures: Beurskens: Genmab: Employment. de Jong:Genmab: Employment. Verploegen:Genmab: Employment. Voorhorst:Genmab: Employment. Strumane:Genmab: Employment. Lindorfer:Genmab: Research Funding. Taylor:Genmab: Research Funding. Schuurman:Genmab: Employment. Parren:Genmab: Employment.
Blue and green edge-emitting laser diodes and vertical-cavity surface emitting lasers on C-plane GaN substrates (2023)

This paper reports the latest device performance of high-power blue and green edge-emitting Laser Diodes (LDs). The epitaxial layers of LDs were grown by Metal Organic Chemical Vapor Deposition (MOCVD) on C-plane free-standing GaN substrates. And a ridge type structure was formed at the top of p-type layers. Fabricated every LD chip was mounted on a heat sink using a junction down method in a TO-Φ9 mm package. We optimized the epitaxial and the device structures for high efficiency and high optical output power. A new developed 455 nm blue LD showed the optical output power and the voltage of 5.90 W and 3.81 V at the forward current of 3 A under Continuous Wave (CW) operation. The wall-plug efficiency (WPE) of the 455 nm blue LD was 51.6 % at 3 A. This is the highest WPE reported so far. The peak WPE of the 455 nm LD was 52.4 % at the forward current of 2.2 A. And a new developed 525 nm green LD showed the optical output power and the voltage of 1.86 W and 4.12 V at the forward current of 1.9 A under CW operation. The wall-plug efficiency (WPE) of the 525 nm green LD was 23.8 % at 1.9 A. This is the highest WPE reported so far. The peak WPE of the 525 nm LD was 25.9 % at the forward current of 1.1 A.
Guest Editorial Wireless Transmission of Information and Power—Part I (2018)

Wireless transmission of information and power has received growing attention in the research community in the past few years. In two consecutive special issues, a total of thirty papers present state-of-the-art results in the broad area of wireless transmission of information and power.
Figure S1 from PP2A Inactivation Mediated by <i>PPP2R4</i> Haploinsufficiency Promotes Cancer Development (2023)

&lt;p&gt;Optimalisation of phosphorylase-a phosphatase activity assays in total brain lysates, using PP1 and PP2A specificity controls: time and concentration courses&lt;/p&gt;
Deep Convolutional Features for Image Based Retrieval and Scene\n Categorization (2015)

Several recent approaches showed how the representations learned by\nConvolutional Neural Networks can be repurposed for novel tasks. Most commonly\nit has been shown that the activation features of the last fully connected\nlayers (fc7 or fc6) of the network, followed by a linear classifier outperform\nthe state-of-the-art on several recognition challenge datasets. Instead of\nrecognition, this paper focuses on the image retrieval problem and proposes a\nexamines alternative pooling strategies derived for CNN features. The presented\nscheme uses the features maps from an earlier layer 5 of the CNN architecture,\nwhich has been shown to preserve coarse spatial information and is semantically\nmeaningful. We examine several pooling strategies and demonstrate superior\nperformance on the image retrieval task (INRIA Holidays) at the fraction of the\ncomputational cost, while using a relatively small memory requirements. In\naddition to retrieval, we see similar efficiency gains on the SUN397 scene\ncategorization dataset, demonstrating wide applicability of this simple\nstrategy. We also introduce and evaluate a novel GeoPlaces5K dataset from\ndifferent geographical locations in the world for image retrieval that stresses\nmore dramatic changes in appearance and viewpoint.\n
Frequency statistics of words used in Japanese food records of FoodLog (2014)

Recording foods enable us to improve our dietary habits. In food records, there are a variety of descriptions of meals because there is no standard way to express meal names. In this study, we analyze Japanese food records from the view of word frequency. We show very small numbers of words are satisfactory to describe the majority of the record.
Determining the position of a single spin relative to a metallic nanowire (2021)

The nanoscale localization of individual paramagnetic defects near an electrical circuit is an important step for realizing hybrid quantum devices with strong spin-microwave photon coupling. Here, we fabricate an array of individual nitrogen vacancy (NV) centers in diamond near a metallic nanowire deposited on top of the substrate. We determine the relative position of each NV center with ∼10 nm accuracy, using it as a vector magnetometer to measure the field generated by passing a DC through the wire.
Fe-incorporated TiO2 nanotube arrays: Electronic structure and magnetic response (2018)

Incorporating Fe atoms into the lattice is shown to significantly alter electronic and magnetic properties of $\mathrm{Ti}{\mathrm{O}}_{2}$ nanotubes synthesized by electrochemical anodization of Ti-Fe alloy sheets. The effects of Fe incorporation on the nanotube morphology, crystallinity, crystal structure, magnetic behavior and electronic structure were investigated with crystallographic and magnetic probes, including synchrotron-based spectroscopy. Results indicate that the iron cations predominately adopt the $\mathrm{F}{\mathrm{e}}^{3+}$ configuration, leading to a large increase of the electronic density of states at the Fermi energy. This increase is anticipated to provide enhanced catalytic action, for instance, in the degradation of water and of air pollutants. These results provide insight for tailoring the functionality of these nanostructures for energy-related applications.
Transformation of Rabbit Lymphocytes by Specific Antigens. (1967)

Lymphocytes from the blood of rabbits immunized with extracts of bovine or rat heart or with human IgG, reacted in vitro when cultured with the specific antigen. The reaction consisted of intense blast transformation, mitotic activity and incorporation of H3-thymidine. The level of response was found to be dose-dependent. Some unstimulated (control) cultures showed moderate “spontaneous” blast formation, without, however, mitotic activity or thymidine uptake.
Role of Mesenchymal Stem Cell in Identification of Hemotoxic Effect of sFas in cases of Nephrotoxicity (2020)

Nephrotoxic nephritis is a clinical condition with a high mortality rate. People with nephritis are susceptible to anemia. This may be due to the presence of sFas.. MSCs hold therapeutic potential in repairing tubular injury, ameliorating renal function deficits, and prolonging survival in acute renal injury. The aim of the present study was to investigate role of BM-derived MSCs cell therapy on mercuric chloride experimentally induced nephrotoxicity and their effect on sFas level in an in vivo albino rat animal model. 90 adult female albino rats were divided equally and randomly in to 3 groups. Group I -ve control group, Group II received an intramuscular injection of Mercuric chloride for 2 weeks (5 days/ week) (1 mg/kg body weight).Group III received an intramuscular injection of Mercuric chloride for 2 weeks (5 days/ week) (1 mg/kg body weight). After establishment of nephrotoxicity by kidney function test (KFT), single dose of labeled MSCs with GFP (Green Fluorescent Protein) injected in the tail vein at a dose of labeled MSCs 1× 106 cells After 2 weeks from 24 hours after last mercuric chloride dose injection, and 3 weeks from labeled MSCs injection. 15 rats from each group were suspected to Blood samples collection from infraorbital. Rats from each animal group were suspected for biochemical estimation of kidney function test (KFT); serum creatinine, Blood Urea Nitrogen (BUN) and complete blood count (CBC) then rats were sacrificed. Kidney were subjected to histopathologic examination by H&E and immunohistochemical examination by PCNA and sFas expression. MSCs suppressed serum urea and creatinine levels in HgCl2-treated rats with increased tissue regeneration and inhibition of tubular cell apoptosis, with decreased expression of PCNA and sFas and increased of the RBCs, Hct and Hb levels compared to the mercuric chloride group reflecting the improvement of anemia.
Monitoring Enzymatic Reactions in Real Time Using Venturi Easy Ambient Sonic-Spray Ionization Mass Spectrometry (2016)

We developed a technique to monitor spatially confined surface reactions with mass spectrometry under ambient conditions, without the need for voltage or organic solvents. Fused-silica capillaries immersed in an aqueous solution, positioned in close proximity to each other and the functionalized surface, created a laminar flow junction with a resulting reaction volume of ∼5 pL. The setup was operated with a syringe pump, delivering reagents to the surface through a fused-silica capillary. The other fused-silica capillary was connected to a Venturi easy ambient sonic-spray ionization source, sampling the resulting analytes at a slightly higher flow rate compared to the feeding capillary. The combined effects of the inflow and outflow maintains a chemical microenvironment, where the rate of advective transport overcomes diffusion. We show proof-of-concept where acetylcholinesterase was immobilized on an organosiloxane polymer through electrostatic interactions. The hydrolysis of acetylcholine by acetylcholinesterase into choline was monitored in real-time for a range of acetylcholine concentrations, fused-silica capillary geometries, and operating flow rates. Higher reaction rates and conversion yields were observed with increasing acetylcholine concentrations, as would be expected.
Three-dimensional simulation of a flapping flag in a uniform flow (2010)

A three-dimensional computational model is developed for simulating the flag motion in a uniform flow. The nonlinear dynamics of the coupled fluid–flag system after setting up of flapping is investigated by a series of numerical tests. At low Reynolds numbers, the flag flaps symmetrically about its centreline when gravity is excluded, and the bending in the spanwise direction is observed near the corners on the trailing edge. As the Reynolds number increases, the spanwise bending is flattened due to the decrease of the positive pressure near the side edges as well as the viscous force of the fluid. At a certain critical Reynolds number, the flag loses its symmetry about the centreline, which is shown to be related to the coupled fluid–flag instability. The three-dimensional vortical structures shed from the flag show a significant difference from the results of two-dimensional simulations. Hairpin or O-shaped vortical structures are formed behind the flag by connecting those generated at the flag side edges and the trailing edge. Such vortical structures have a stabilization effect on the flag by reducing the pressure difference across the flag. Moreover, the positive pressure near the side edges is significantly reduced as compared with that in the center region, causing the spanwise bending. The Strouhal number defined based on the flag length is slightly dependent on the Reynolds number and the flag width, but scales with the density ratio as St ~ ρ −1/2 ). On the other hand, the flapping-amplitude-based Strouhal number remains close to 0.2, consistent with the values reported for flying or swimming animals. A flag flapping under gravity is then simulated, which is directed along the negative spanwise direction. The sagging down of the flag and the rolling motion of the upper corner are observed. The dual effects of gravity are demonstrated, i.e. the destabilization effect like the flag inertia and the stabilization effect by increasing the longitudinal tension force.