gebhart/patrepeval huggingface dataset:
Isoforms of MUC16 activate oncogenic signaling through EGF receptors to enhance the progression of pancreatic cancer (2020)

Aberrant expression of CA125/MUC16 is associated with pancreatic ductal adenocarcinoma (PDAC) progression and metastasis. However, knowledge of the contribution of MUC16 to pancreatic tumorigenesis is limited. Here, we show that MUC16 expression is associated with disease progression, basal-like and squamous tumor subtypes, increased tumor metastasis, and short-term survival of PDAC patients. MUC16 enhanced tumor malignancy through the activation of AKT and GSK3β oncogenic signaling pathways. Activation of these oncogenic signaling pathways resulted in part from increased interactions between MUC16 and epidermal growth factor (EGF)-type receptors, which were enhanced for aberrant glycoforms of MUC16. Treatment of PDAC cells with monoclonal antibody (mAb) AR9.6 significantly reduced MUC16-induced oncogenic signaling. mAb AR9.6 binds to a unique conformational epitope on MUC16, which is influenced by O-glycosylation. Additionally, treatment of PDAC tumor-bearing mice with either mAb AR9.6 alone or in combination with gemcitabine significantly reduced tumor growth and metastasis. We conclude that the aberrant expression of MUC16 enhances PDAC progression to an aggressive phenotype by modulating oncogenic signaling through ErbB receptors. Anti-MUC16 mAb AR9.6 blocks oncogenic activities and tumor growth and could be a novel immunotherapeutic agent against MUC16-mediated PDAC tumor malignancy.
Overall and Adiabatic Effectiveness Values on a Scaled Up, Simulated Gas Turbine Vane: Part II—Numerical Simulations (2011)

Conjugate heat transfer (CHT) calculation techniques for the heat transfer analysis of high-pressure turbines (HPT) have been developed during the past few years. Thus, it has become possible to take into account the coupling of the film, internal cooling, external gas flow and the metal diffusive heat transfer. The coupling problem may become extremely important in regions such as the airfoil leading edge and the vicinity of film hole breakout region where heat fluxes and thermal gradients are high. This article presents the results obtained using fully coupled 3D CHT simulations of a simplified film-cooled leading edge model and a NASA C3X vane with suction side film cooling. The results for the two cases are compared against experimental data obtained at University of Texas at Austin. The numerical simulations were conducted using the k-ω turbulence model. The leading edge model overall effectiveness predictions are in good agreement with the experiments, especially in the low blowing ratio range (1≤M≤2). For the C3X vane, the CHT results tend to underpredict the midspan and laterally averaged effectiveness due to film liftoff. However, the quantitative agreement is still reasonably good. The different levels of overall effectiveness agreement found between all cases are discussed.
Response to Berry et al’s “Cutaneous small-vessel vasculitis following single-dose Janssen Ad26.COV2.S vaccination” (2022)

To the Editor: On February 27, 2021, the United States Food and Drug Administration issued an Emergency Use Authorization for the Janssen adenovirus vector COVID-19 vaccine.1-2 The most commonly reported adverse events of the Janssen vaccine include mild reactogenicity symptoms, including location injection site reactions, hypersensitivity, and systemic reactogenicity, like fatigue and headache.2 Prior vaccines, including adenovirus vector vaccines and influenza, have reported small- and medium-vessel vasculitis as potential adverse events.
Effect of BMP-2 Adherent to Resorbable Sutures on Cartilage Repair: A Rat Model of Xyphoid Process (2020)

Meniscal tears are often seen in orthopedic practice. The current strategy for meniscal repair has only had limited success with a relatively high incidence of re-operative rate. This study evaluates the therapeutic effects of Bone morphogenetic protein-2 (BMP-2) soaked sutures for cartilage repair, using a rat model of xyphoid healing. Vicryl-resorbable sutures were presoaked in BMP-2 solutions prior to animal experimentation. Rat xyphoid process (an avascular hyaline cartilage structure) was surgically ruptured followed by repair procedures with regular suture or with sutures that were pre-soaked in BMP-2 solutions. In vitro assessment indicated that presoaking the Vicryl-resorbable sutures with 10 µg/mL BMP-2 resulted in a sustained amount of the growth factor release up to 7 days. Histological analysis suggested that application of this BMP-2 soaked suture on the rat xyphoid process model significantly improved the avascular cartilage healing compared to non-soaked control sutures. In conclusion, data here confirm that the rat xyphoid process repair is a reproducible and inexpensive animal model for meniscus and other cartilage repair. More importantly, coating of BMP-2 on sutures appears a potential avenue to improve cartilage repair and regeneration. Further study is warranted to explore the molecular mechanisms of this strategy.
High-Power Fiber Laser (1991)

We discuss a theoretical feasibility of a development of effective fiber lasers with an average output power of tens of watts, and we demonstrate experimentally an average power of 3.9 W in the 1.54 um spectral range for the Er3+-doped quartz fiber laser. For a single mode fiber laser an output power of about 1 W has achieved
Intestinal spirochetosis and chronic watery diarrhea: Clinical and histological response to treatment and long‐term follow up (2006)

Histological intestinal spirochetosis appears to be relatively uncommon in Catalonia (Spain) compared to previous reports from other countries, but was identified in patients (0.7%) with chronic watery diarrhea. Sustained clinical recovery after spontaneous or drug-induced spirochetal disappearance in these individuals suggests that intestinal spirochetosis may play a pathogenic role in chronic watery diarrhea. Treatment with metronidazole is advisable in patients with persistent symptoms.
Safety of Adalimumab in Global Clinical Trials of Patients with Crohnʼs Disease (2010)

Purpose: Adalimumab (ADA), a fully human anti-TNF monoclonal antibody, is approved in Europe for treating patients with severely active Crohn's disease (CD) who have had an inadequate response to conventional therapy. Methods: All patients with CD participating in the pivotal randomized controlled clinical trials, open-label extensions, and Phase IIIb studies were evaluated for safety at regular intervals. Rates of adverse events (AEs) of interest to physicians prescribing anti-TNF therapy were assessed per 100-patient-years (100-PYs) of ADA exposure. Standardized mortality ratios were calculated using the World Health Organization 2003 age- and sex-matched US mortality data. Results: As of 6 November, 2009, the ADA CD clinical trial safety database contained data for 3603 patients (4088-PYs of ADA exposure). Table 1 compares AE rates observed in all clinical trials in CD at the 6 November 2009 cutoff to those from the 15 April 2008 safety update.1 Observed cases of lymphoma (2), tuberculosis (3), congestive heart failure (2), and demyelinating disease (6) have not increased since the previous update. The AE rates were comparable to those reported in rheumatoid arthritis trials.2 The calculated standardized mortality ratio in ADA-treated patients with CD, 0.48 (95% confidence interval [CI]: 0.15, 1.12), is lower than the rate of 1.52 (95% CI: 1.32, 1.74) reported in a recent meta-analysis of patients with CD.3 Conclusion: The safety profile and AE rates for ADA in CD were similar to those reported previously for ADA and other TNF antagonists in CD populations.4,5 ADA was well-tolerated, and no new safety signals were identified. References: 1. Colombel DDW 2009; Poster W1089. 2. Burmester Ann Rheum Dis 2009; 68:1863 3. Canavan Aliment Pharmacol Ther 2007; 25:861 4. Colombel Gastroenterology 2004; 126:19 5. Lichtenstein Clin Gastroenterol Hepatol 2006; 4:621. Disclosure: Dr Colombel - Consultant: Abbott Dr Panaccione - Consultant, research support, lecture fees: Abbott Dr Sandborn - Consultant, research support: Abbott Dr Rutgeerts - Consultant, research support: Abbott Dr Hanauer - Consultant: Abbott Dr Reinisch - Consultant: Abbott Dr Robinson - Employee: Abbott Dr Lau - Employee: Abbott Dr Cardoso - Employee: Abbott Dr Pollack - Employee: Abbott.Table: [1142]
Preparation of fire-resistant poly(styrene-co-acrylonitrile) foams using supercritical CO2 technology (2010)

This work deals with the preparation and characterization of fire-resistant poly(styrene-co-acrylonitrile) (SAN) foams containing (organo)clays and/or melamine polyphosphate (MPP) as fire retardants using supercritical CO2 as the foaming agent. The additives dispersion was first characterized with X-ray and transmission electron microscopy (TEM) analyses. Their presence clearly affected the cellular morphology, as observed by scanning electron microscopy (SEM). Then, the peak of heat release rate (PHRR) and total heat evolved (THE) were determined with a cone calorimetry test, performed on each foamed sample as a function of the foam density. Incorporation of clay (3 and 5 wt%) in the exfoliated state into the SAN foam clearly led to a significant decrease of PHRR, while intercalated and aggregated clay had a lower effect. Similar results were obtained with 10 and 20 wt% of MPP. The best results were obtained when exfoliated clay and MPP were combined, with a PHRR drop as large as 75%, thanks to the synergistic action of both additives. The magnitude of PHRR drop, related to the fire resistance, was found to be in direct relationship with the cohesiveness of the protective carbonaceous layer formed at the sample surface during combustion. Clay and MPP, when added together, are thus believed to favour the formation of a highly cohesive protective layer able to act as an efficient shield against the flame, despite the fact that the sample is originally composed of ∼90% of voids.
Presenilin 1 mediates the turnover of telencephalin in hippocampal neurons via an autophagic degradative pathway (2004)

Presenilin 1 (PS1) interacts with telencephalin (TLN) and the amyloid precursor protein via their transmembrane domain (Annaert, W.G., C. Esselens, V. Baert, C. Boeve, G. Snellings, P. Cupers, K. Craessaerts, and B. De Strooper. 2001. Neuron. 32:579-589). Here, we demonstrate that TLN is not a substrate for gamma-secretase cleavage, but displays a prolonged half-life in PS1(-/-) hippocampal neurons. TLN accumulates in intracellular structures bearing characteristics of autophagic vacuoles including the presence of Apg12p and LC3. Importantly, the TLN accumulations are suppressed by adenoviral expression of wild-type, FAD-linked and D257A mutant PS1, indicating that this phenotype is independent from gamma-secretase activity. Cathepsin D deficiency also results in the localization of TLN to autophagic vacuoles. TLN mediates the uptake of microbeads concomitant with actin and PIP2 recruitment, indicating a phagocytic origin of TLN accumulations. Absence of endosomal/lysosomal proteins suggests that the TLN-positive vacuoles fail to fuse with endosomes/lysosomes, preventing their acidification and further degradation. Collectively, PS1 deficiency affects in a gamma-secretase-independent fashion the turnover of TLN through autophagic vacuoles, most likely by an impaired capability to fuse with lysosomes.
The impact of human EGFR kinase domain mutations on lung tumorigenesis and in vivo sensitivity to EGFR-targeted therapies (2021)

SummaryTo understand the role of human epidermal growth factor receptor (hEGFR) kinase domain mutations in lung tumorigenesis and response to EGFR-targeted therapies, we generated bitransgenic mice with inducible expression in type II pneumocytes of two common hEGFR mutants seen in human lung cancer. Both bitransgenic lines developed lung adenocarcinoma after sustained hEGFR mutant expression, confirming their oncogenic potential. Maintenance of these lung tumors was dependent on continued expression of the EGFR mutants. Treatment with small molecule inhibitors (erlotinib or HKI-272) as well as prolonged treatment with a humanized anti-hEGFR antibody (cetuximab) led to dramatic tumor regression. These data suggest that persistent EGFR signaling is required for tumor maintenance in human lung adenocarcinomas expressing EGFR mutants.
Abstract 520: Local changes in tissue microenvironment regulate mammary tumor progression (2011)

Abstract Breast cancer is one of the most prominent cancers in women with approximately 40,000 women dying each year of the disease in the United States alone. Although cancer has a strong genetic basis, the tissue microenvironment also plays a significant role in tumor formation and progression. To explore this process in greater detail, we analyzed tissue structure, cellular biochemistry and gene expression change during tumor progression in a previously developed FVB C3(1)-SV40Tag transgenic mouse model in which the females spontaneously develop mammary tumors that undergo changes in cell tissue morphology that closely mimic those observed during human breast cancer progression. Histological characterization of breast tissues isolated from 8, 12, 16 and 20 week old female mice confirmed that this model is extremely robust with normal glandular morphology being observed at 8 weeks, ductal carcinoma in situ (DCIS) morphology appearing at 12 weeks and 16 weeks and large tumors with metastatic lesions by 20 weeks of age. More careful analysis revealed that tumor formation did not scale directly with expression of SV40 large T antigen that is the drive gene in this transgenic model, and instead appeared to be more closely related to variations in the tissue microenvironment. This was supported by the observation that the number of myoepithelial cells and expression levels of known breast cancer differentiation markers, including estrogen and progesterone receptors, varied among ducts within tissues at each time point even though the majority of epithelial cells in these ducts expressed T antigen. Gene microarray analysis of mammary tissues from these time points revealed consistent changes in genome-wide expression profiles, and several interesting candidates are currently being analyzed regarding their potential involvement in early microenvironmental changes that might drive the tumor progression. In summary, our preliminary results showed that despite expression of constitutively active large T antigen in the breast epithelial cells of these transgenic mice, we observe heterogeneous tumor formation within mammary tissues. These local variations in tumor progression may be related to regional changes in stromal and extracellular matrix components that are currently being analyzed by immunohistochemical analysis. Our long-term goal is to leverage the information we uncover through this effort to design differentiation therapies that suppress tumor cell growth, induce differentiation and hence, reverse breast cancer progression. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 520. doi:10.1158/1538-7445.AM2011-520
Inhomogeneous vasomotor effects of moderate selective and non-selective endothelin-receptor blockade in stable coronary artery disease (2009)

NCT00427232.
Počeci primjene Međunarodne klasifikacije funkcioniranja, onesposobljenosti i zdravlja (2013)

Svjetska zdravstvena organizacija objavila je novu klasifikaciju s naslovom: Međunarodna klasifikacija funkcioniranja, onesposobljenosti i zdravlja (MKF). Radi se o klasifikaciji koja pruža osnovu za procjenu funkcioniranja osobe te postoji preporuka za njeno komplementarno koristenje sa Međunarodnom klasifikacijom bolesti i srodnih zdravstvenih problema (MKB-10). Ovakvo komplementarno koristenje pruža potpuniju sliku o zdravstvenom stanju osobe te njenoj sposobnosti za sudjelovanje u svakodnevnom životu. Prijevod na hrvatski jezik pripremili su djelatnici Hrvatskog zavoda za javno zdravstvo, Ministarstva zdravlja te predstavnici osoba s invaliditetom. Prijevod MKF na hrvatski jezik je publiciran, a zapocelo se i sa njenom primjenom u unapređenju sustava vjestacenja za osobe s invaliditetom te fizioterapijskoj djelatnosti.
Separation of related opiate compounds using capillary electrochromatography (2000)

Capillary electrophoretic separations have been investigated for six controlled narcotic analgesic compounds having related structures. Owing to the similar charge-to-mass ratios of these compounds, capillary zone electrophoresis failed to provide a satisfactory separation, whereas a baseline-resolved separation was achieved in 10 min using micellar electrokinetic chromatography. Column efficiencies of 40,000-150,000 plates/m were obtained with a 50 cm long, 50 microm inner diameter (ID) capillary using 50 mM sodium dodecyl sulfate (SDS) in a 50 mM borate solution containing 12% isopropanol. In contrast, separation of this mixture by capillary electrochromatography proved to be significantly superior. The capillary was 15 cm long, with an ID of 75 microm, and was packed with 1.5 microm nonporous octadecyl silica (ODS) particles. The mobile phase consisted of 80% 10 mM tris(hydroxymethyl)aminomethane (Tris) and 20% acetonitrile, and contained 5 mM SDS. A complete separation was obtained in 2.5 min with an efficiency of 250,000-500,000 plates/m.
The FcγRIa (CD64) Ligand Binding Chain Triggers Major Histocompatibility Complex Class II Antigen Presentation Independently of Its Associated FcR γ-Chain (1999)

Within multi-subunit Ig receptors, the FcR γ-chain immunoreceptor tyrosine-based activation motif (ITAM) plays a crucial role in enabling antigen presentation. This process involves antigen-capture and targeting to specific degradation and major histocompatibility complex (MHC) class II loading compartments. Antigenic epitopes are then presented by MHC class II molecules to specific T cells. The high-affinity receptor for IgG, hFcγRIa, is exclusively expressed on myeloid lineage cells and depends on the FcR γ-chain for surface expression, efficient ligand binding, and most phagocytic effector functions. However, we show in this report, using the IIA1.6 cell model, that hFcγRIa can potentiate MHC class II antigen presentation, independently of a functional FcR γ-chain ITAM. Immunoelectron microscopic analyses documented hFcγRIa -chain/rabbit IgG-Ovalbumin complexes to be internalized and to migrate via sorting endosomes to MHC class II-containing late endosomes. Radical deletion of the hFcγRIa -chain cytoplasmic tail did not affect internalization of rabbit IgG-Ovalbumin complexes. Importantly, however, this resulted in diversion of receptor-ligand complexes to the recycling pathway and decreased antigen presentation. These results show the hFcγRIa cytoplasmic tail to contain autonomous targeting information for intracellular trafficking of receptor-antigen complexes, although deficient in canonical tyrosine- or dileucine-targeting motifs. This is the first documentation of autonomous targeting by a member of the multichain FcR family that may critically impact the immunoregulatory role proposed for hFcγRIa (CD64).
Intramolecular vibrational relaxation in 1,4 dioxane (1984)

Intramolecular vibrational energy transfer in isolated gas phase molecules of dioxane has been studied by spectrally resolving the IR laser induced fluorescence from C–H stretch fundamentals, combination bands, and overtones in the region from 2800 to 3000 cm−1. By comparing the experimentally observed relative energy content of the IR active modes with that calculated from a statistical model it is concluded that substantial randomization of the energy initially deposited in the zeroth order optically excited state occurs. This statistical distribution differs from a thermal distribution due to fluctuations in the local state density. The energy content distribution is best described by the model if coupling over a range of 4 cm−1 is assumed. Dilution factors for the various C–H stretch modes were observed which vary from 0.018 to 0.13. These values are dependent on the rotational state excited. Collisional time dependent spectra of several relaxed modes are presented. The experiments are performed by exciting molecules cooled in a nozzle expansion with light from an infrared optical parametric oscillator. The fluorescence spectra are recorded with a cryogenic Michelson interferometer.
A Soft Porous Organic Cage Crystal with Complex Gas Sorption Behavior (2011)

Big softy! A soft porous molecular crystal composed of organic cages exhibits complex multistep gas sorption isotherms (see figure), analogous to those observed in soft porous metal–organic frameworks. Softness is induced by frustrated packing of the cages and structural flexibility leads to kinetic guest trapping. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Challenges to via middle TSV integration at sub-28nm nodes (2014)

This work presents the via middle TSV integration at sub-28 nm nodes using a new local interconnect scheme involving V0 vias. Various V0 schemes are presented along with their respective resistance, capacitance and leakage current data. The characterization and reliability results are presented through TSV daisy chain structures and MOL via chains.
Design and Assessment of Sound Source Localization System with a UAV-Embedded Microphone Array (2017)

[abstFig src='/00290001/15.jpg' width='300' text='Visualization of localization result' ] We have studied on robot-audition-based sound source localization using a microphone array embedded on a UAV (unmanned aerial vehicle) to locate people who need assistance in a disaster-stricken area. A localization method with high robustness against noise and a small calculation cost have been proposed to solve a problem specific to the outdoor sound environment. In this paper, the proposed method is extended for practical use, a system based on the method is designed and implemented, and results of sound source localization conducted in the actual outdoor environment are shown. First, a 2.5-dimensional sound source localization method, which is a two-dimensional sound source localization plus distance estimation, is proposed. Then, the offline sound source localization system is structured using the proposed method, and the accuracy of the localization results is evaluated and discussed. As a result, the usability of the proposed extended method and newly developed three-dimensional visualization tool is confirmed, and a change in the detection accuracy for different types or distances of the sound source is found. Next, the sound source localization is conducted in real-time by extending the offline system to online to ensure that the detection performance of the offline system is kept in the online system. Moreover, the relationship between the parameters and detection accuracy is evaluated to localize only a target sound source. As a result, indices to determine an appropriate threshold are obtained and localization of a target sound source is realized at a designated accuracy.
Normalization of anti-cryptococcal activity and interleukin-12 production after highly active antiretroviral therapy (2000)

PMNL from HIV-infected patients with high viral load have impaired function; HAART normalizes antimicrobial and secretory activities. The effects of HAART on innate immunity provide new prospects for reduction of HAART-mediated opportunistic infections.