gebhart/patrepeval huggingface dataset:
Development of High-Specificity Fluorescent Probes to Enable Cannabinoid Type 2 Receptor Studies in Living Cells (2020)

Pharmacological modulation of cannabinoid type 2 receptor (CB2R) holds promise for the treatment of numerous conditions, including inflammatory diseases, autoimmune disorders, pain, and cancer. Despite the significance of this receptor, researchers lack reliable tools to address questions concerning the expression and complex mechanism of CB2R signaling, especially in cell-type and tissue-dependent context. Herein, we report for the first time a versatile ligand platform for the modular design of a collection of highly specific CB2R fluorescent probes, used successfully across applications, species and cell types. These include flow cytometry of endogenously expressing cells, real-time confocal microscopy of mouse splenocytes and human macrophages, as well as FRET-based kinetic and equilibrium binding assays. High CB2R specificity was demonstrated by competition experiments in living cells expressing CB2R at native levels. The probes were effectively applied to FACS analysis of microglial cells derived from a mouse model relevant to Alzheimer’s disease and to the detection of CB2R in human breast cancer cells.
Analysis of lung tumor initiation and progression in transgenic mice for Cre‐inducible overexpression of Cul4A gene (2015)

Our findings indicate that Cul4A is oncogenic in vivo, and this mouse model is a tool in understanding the mechanisms of Cul4A in human cancers and for testing experimental therapies targeting Cul4A.
Induction of IL-15 by TCR/CD3 aggregation depends on IFN-γand protects against apoptosis of immature thymocytesin vivo (2002)

TCR/CD3 aggregation by injection of anti-CD3 Ab produces T cell activation, release of cytokines such as IFN-gamma, and apoptosis in the cortical region of the thymus. We show that anti-CD3 Ab induces IL-15 mRNA in spleens of wild-type but not IFN-gamma receptor-knock-out (IFN-gammaR KO) mice. The loss of IL-15 mRNA induction in IFN-gammaR KO mice was associated with increased thymocyte apoptosis. Pretreatment of wild-type mice with neutralizing anti-IL-15 Ab increased the anti-CD3-triggered thymocyte apoptosis, thus mimicking the sensitive phenotype of IFN-gammaR KO mice. Inversely, anti-CD3-induced apoptosis in IFN-gammaR KO mice was suppressed by administration of recombinant IL-15. In IFN-gammaR KO mice and in wild-type mice that were treated with anti-IL-15, augmented apoptosis affected mainly CD4+CD8+ immature thymocytes. IL-15 as well as IL-15Ralpha mRNA expression in thymocytes was not increased by anti-CD3. These data demonstrate that systemic IL-15 exerts anti-apoptotic activity on immature T cells and establish a regulatory mechanism whereby TCR/CD3 engagement induces IL-15 expression via an IFN-gamma-dependent pathway. The self-amplifying nature of this IFN-gamma/IL-15 connection may constitute a regulatory pathway in central tolerance to self.
Echocardiography-based AI for detection and quantification of atrial septal defect (2023)

We demonstrated the feasibility of using a deep learning model for automated detection and quantification of ASD from color Doppler echocardiography. This model has the potential to improve the accuracy and efficiency of using color Doppler in clinical practice for screening and quantification of ASDs, that are required for clinical decision making.
Development of a Controller Hardware-in-the-Loop Platform for Microgrid Distributed Control Applications (2018)

Microgrids (MGs) are ideally suited for distributed control solutions. However, implementation and validation of the developed distributed control algorithms are quite challenging. In this paper we propose a Controller Hardware-in-the-Loop (CHIL) platform for MG distributed control applications that satisfy the requirements of IEEE Std. 2030.7 for MG control systems. We describe two main features of the proposed platform: 1) a software platform that enables the implementation of control algorithms that have been developed analytically and 2) a real-time MG testbed that replicates practical MG operation environment by using real-time communication network and grid solutions. Implementation and validation of a distributed MG synchronization operation control strategy are used to demonstrate the performance of the proposed CHIL platform.
Combination of Centralized & Decentralized Database and Terminal-based Spectrum Sensing for Secondary Spectrum Access (2010)

International audience
Charge transport physics of a unique class of rigid-rod conjugated polymers with fused-ring conjugated units linked by double carbon-carbon bonds (2021)

We investigate the charge transport physics of a previously unidentified class of electron-deficient conjugated polymers that do not contain any single bonds linking monomer units along the backbone but only double-bond linkages. Such polymers would be expected to behave as rigid rods, but little is known about their actual chain conformations and electronic structure. Here, we present a detailed study of the structural and charge transport properties of a family of four such polymers. By adopting a copolymer design, we achieve high electron mobilities up to 0.5 cm<sup>2</sup> V<sup>-1</sup> s<sup>-1</sup> Field-induced electron spin resonance measurements of charge dynamics provide evidence for relatively slow hopping over, however, long distances. Our work provides important insights into the factors that limit charge transport in this unique class of polymers and allows us to identify molecular design strategies for achieving even higher levels of performance.
Integration of a Rib Waveguide Distributed Feedback Structure into a Light‐Emitting Polymer Field‐Effect Transistor (2009)

DOI: 10.1002/adfm.200801897 The list of affiliations for the authors of this paper is not correct. The correct list is as follows:
ChemInform Abstract: THERMISCHE UND PHOTOCHEMISCHE DIMERISIERUNG VON NORBORNADIEN MIT NICKELTETRACARBONYL ALS KATALYSATOR (1972)

Abstract Norbomadien (I) reagiert in Substanz in Gegenwart von Nickeltetracarbonyl (II) zu den beiden Cyclobutandimeren (III) und (IV), bei der Photolyse entstehen isomere Formen (V)‐(VIII).
Efficient design optimization of complex electromagnetic systems using parametric macromodeling techniques (2011)

We propose a new parametric macromodeling technique for complex electromagnetic systems described by scattering parameters, which are parameterized by multiple design variables such as layout or substrate feature. The proposed technique is based on an efficient and reliable combination of rational identification, a procedure to find scaling and frequency shifting system coefficients, and positive interpolation schemes. Parametric macromodels can be used for efficient and accurate design space exploration and optimization. A design optimization example for a complex electromagnetic system is used to validate the proposed parametric macromodeling technique in a practical design process flow.
ChemInform Abstract: SYNTHESIS OF 2,3,5,6‐TETRAHYDROIMIDAZO(1,2‐C)QUINAZOLINE DERIVATIVES AS POTENTIAL NARCOTIC ANTAGONISTS (1980)

Abstract Aus o‐Amino‐benzonitril (I) entstehen durch entsprechende Alkylierung die Substitutionsprodukte (II), die mit Ethylendiamin (III) zu (IV) reagieren.
2006, Volume 18 Decyl-End-Capped Thiophene−Phenylene Oligomers as Organic Semiconducting Materials with Improved Oxidation Stability. (2006)

ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionORIGINAL ARTICLEThis notice is a correction2006, Volume 18 Decyl-End-Capped Thiophene−Phenylene Oligomers as Organic Semiconducting Materials with Improved Oxidation Stability.Segei A. Ponomarenko, Stephan Kirchmeyer, Andreas Elschner, Ninel M. Alpatova, Marcus Halik, Hagen Klauk, Ute Zschieschang, and Günter SchmidCite this: Chem. Mater. 2006, 18, 7, 2004Publication Date (Web):March 9, 2006Publication History Published online9 March 2006Published inissue 1 April 2006https://pubs.acs.org/doi/10.1021/cm060401zhttps://doi.org/10.1021/cm060401zcorrectionACS PublicationsCopyright © 2006 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views449Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (18 KB) Get e-Alertsclose Get e-Alerts
Data from Distinctive Subpopulations of Stromal Cells Are Present in Human Lymph Nodes Infiltrated with Melanoma (2023)

&lt;div&gt;Abstract&lt;p&gt;Metastasis of human tumors to lymph nodes (LN) is a universally negative prognostic factor. LN stromal cells (SC) play a crucial role in enabling T-cell responses, and because tumor metastases modulate their structure and function, this interaction may suppress immune responses to tumor antigens. The SC subpopulations that respond to infiltration of malignant cells into human LNs have not been defined. Here, we identify distinctive subpopulations of CD90&lt;sup&gt;+&lt;/sup&gt; SCs present in melanoma-infiltrated LNs and compare them with their counterparts in normal LNs. The first population (CD90&lt;sup&gt;+&lt;/sup&gt; podoplanin&lt;sup&gt;+&lt;/sup&gt; CD105&lt;sup&gt;+&lt;/sup&gt; CD146&lt;sup&gt;+&lt;/sup&gt; CD271&lt;sup&gt;+&lt;/sup&gt; VCAM-1&lt;sup&gt;+&lt;/sup&gt; ICAM-1&lt;sup&gt;+&lt;/sup&gt; α-SMA&lt;sup&gt;+&lt;/sup&gt;) corresponds to fibroblastic reticular cells that express various T-cell modulating cytokines, chemokines, and adhesion molecules. The second (CD90&lt;sup&gt;+&lt;/sup&gt; CD34&lt;sup&gt;+&lt;/sup&gt; CD105&lt;sup&gt;+&lt;/sup&gt; CD271&lt;sup&gt;+&lt;/sup&gt;) represents a novel population of CD34&lt;sup&gt;+&lt;/sup&gt; SCs embedded in collagenous structures, such as the capsule and trabeculae, that predominantly produce extracellular matrix. We also demonstrated that these two SC subpopulations are distinct from two subsets of human LN pericytes, CD90&lt;sup&gt;+&lt;/sup&gt; CD146&lt;sup&gt;+&lt;/sup&gt; CD36&lt;sup&gt;+&lt;/sup&gt; NG2&lt;sup&gt;−&lt;/sup&gt; pericytes in the walls of high endothelial venules and other small vessels, and CD90&lt;sup&gt;+&lt;/sup&gt; CD146&lt;sup&gt;+&lt;/sup&gt; NG2&lt;sup&gt;+&lt;/sup&gt; CD36&lt;sup&gt;−&lt;/sup&gt; pericytes in the walls of larger vessels. Distinguishing between these CD90&lt;sup&gt;+&lt;/sup&gt; SC subpopulations in human LNs allows for further study of their respective impact on T-cell responses to tumor antigens and clinical outcomes.&lt;/p&gt;&lt;/div&gt;
Model-Data Intercomparison for Marginal Sea Overflows (2002)

Abstract : The long-term goal of this project is to enhance the level of understanding of the dynamics of overflows, which are characterized by high degree of variability and mixing near strategic straits connecting various marginal seas and oceans.
Development of a Bio-Inspired Acyl-Anion Equivalent Macrocyclization and Synthesis of a trans-Resorcylide Precursor (2007)

Studies on the macrocyclization of alpha,omega-dialdehydes have revealed a strong dependence on ring size with respect to the ultimate efficiency of the reaction. Strong catalyst dependence was observed, as thiazolium salts led to no detectable product formation, whereas electron-deficient triazolium salts served as precatalysts for the cyclization. Surprisingly, the N-pentafluorophenyl triazolium variant led to cyclization at room temperature within a short 90-min reaction time. These findings were applied to a range of substrates, including the synthesis of a key intermediate in a rapid synthesis of trans-resorcylide.
Constrained least squares detector for OFDM/SDMA-based wireless networks (2003)

The two major obstacles toward high-capacity indoor wireless networks are distortion due to the indoor channel and the limited bandwidth which necessitates a high spectral efficiency. A combined orthogonal frequency division multiplexing (OFDM)/spatial division multiple access (SDMA) approach can efficiently tackle both obstacles and paves the way for cheap, high-capacity wireless indoor networks. The channel distortion due to multipath propagation is efficiently mitigated with OFDM while the bandwidth efficiency can be increased with the use of SDMA. However, to keep the cost of an indoor wireless network comparable to its wired counterpart's cost, low-complexity SDMA processors with good performance are of special interest. In this paper, we propose a new multiuser SDMA detector which is designed for constant modulus signals. This constrained least squares (CLS) receiver, which deterministically exploits the constant modulus nature of the subcarrier modulation to achieve better separation, is compared in terms of performance and complexity with the zero forcing (ZF) and the minimum mean square error (MMSE) receiver. Additionally, since the CLS detector relies on reliable channel knowledge at the receiver, we propose a strategy for estimating the multiple input multiple output (MIMO) channels. Simulations for a Hiperlan II-based case-study show that the CLS detector significantly outperforms the ZF detector and comes close to the performance of the MMSE detector for QPSK. For higher order M-PSK, the CLS detector outperforms the MMSF detector. Furthermore, the estimation complexity for the CLS detector is substantially lower than that for the MMSE detector which additionally requires estimation of the noise power.
The gag -specific cytotoxic T lymphocytes in rhesus monkeys infected with the simian immunodeficiency virus of macaques. (1990)

The simian immunodeficiency virus of macaques (SIVmac) is a lentivirus which induces an AIDS-like disease in rhesus monkeys. We have explored the virus-specific cellular immune response in SIVmac-infected rhesus monkeys. Con A-activated, IL-2 expanded PBL of some SIVmac-infected rhesus monkeys lyse autologous B lymphoblastoid cell lines infected with a recombinant vaccinia virus that carries the SIVmac gag gene. This lysis is mediated by CD8+ lymphocytes and is MHC class I restricted. Moreover, these effector lymphocytes do not express the NK cell-associated molecules NKH1 or CD16. These cells are, therefore, CTL. In a limited prospective study of SIVmac-infected rhesus monkeys, the presence of the SIVmac gag-specific CTL activity in PBL correlated with both a reduced efficiency in isolating SIVmac from PBL of these monkeys and their extended survival. This method for assessing SIVmac gag-specific cellular immunity in rhesus monkeys will be important not only in investigating the immunopathogenesis of SIVmac-induced disease, but also in evaluating the capacity of candidate AIDS vaccines to elicit a cell-mediated immune response in this animal model.
Neutral ansa-bis(fluorenyl)silane neodymium borohydrides: synthesis, structural study and behaviour as catalysts in butadiene–ethylene copolymerisation (2010)

The reaction of silylene-bridged bis(fluorenyl)dipotassium salts with neodymium tris(borohydride) afforded new neutral ansa-bis(fluorenyl)silane neodymium borohydrides: (Flu2SiR2)Nd(BH4)(THF) [R2 = Me2, Et2, (CH2)3, Me(Ph), Flu = C13H8] that were better characterised and more soluble than the previously described anionic [(Flu2SiMe2)Nd(BH4)2]−. The X-ray structures of three of these complexes were determined, and their solid-state geometrical parameters are very similar, despite the ring strain introduced by the silacyclobutane bridge in [Flu2Si(CH2)3]Nd(BH4)(THF). The main geometrical features were satisfactorily reproduced by DFT calculations. The catalytic activity of the title complexes in ethylene–butadiene copolymerisation reactions was assessed and compared to that of the reported activity of [(Flu2SiMe2)Nd(BH4)2]− under similar conditions. From these results it can be concluded that the cyclo-copolymerisation of ethylene with butadiene is characteristic of a catalyst featuring silylene-bridged bis(fluorenyl) ligands around neodymium, and appears to be independent of the substituents at the silicon atom.
Pre-safe - the next step in the enhancement of vehicle safety (2003)

In times when the potential for further optimizing passive safety become ever smaller, new ways for improving vehicle safety must be found. Accident analyses shows that the pre-crash phase provides room for substantial improvement, calling for reversible systems that exploit this available time before the accident for the benefit of the occupants. Preventive occupant protection is realized for the first time with PRE-SAFE, initiating the next phase in the enhancement of vehicle safety. PRE-SAFE is embedded in Mercedes-Benz's integrated safety concept, and combines active and passive safety systems. Announced in theory at the ESV 2001, PRESAFE has now seen its first series application in the 2003 S-Class model, and initial field experience has been gathered. Taking nature's reflex as a role model, the vehicle reacts to critical driving conditions identified by the sensors of the Electronic Stability Program (ESP) and the Brake Assist (BAS) that may lead to an accident. Reversible belt-pretensioners for occupant fixation, passenger seat positioning and sunroof closure are activated. However, these are merely first steps in this new and evolving safety technology. Further PRE-SAFE applications might be introduced in the future. Individual safety and environment sensing will mark the next major steps. For the covering abstract see ITRD E825082.
A Transforming Growth Factor-β Receptor–Interacting Protein Frequently Mutated in Human Ovarian Cancer (2005)

Ovarian carcinomas, particularly recurrent forms, are frequently resistant to transforming growth factor-beta (TGF-beta)-mediated growth inhibition. However, mutations in the TGF-beta receptor I and receptor II (TbetaR-I and TbetaR-II) genes have only been reported in a minority of ovarian carcinomas, suggesting that alterations in TGF-beta-signaling components may play an important role in the loss of TGF-beta responsiveness. Using laser-capture microdissection and nested reverse-transcription-PCR, we found that km23, which interacts with the TGF-beta receptor complex, is altered at a high frequency in human ovarian cancer patients. A novel form of km23, missing exon 3 (Deltaexon3-km23), was found in 2 of 19 tumor tissues from patients with ovarian cancer. In addition to this alteration, a stop codon mutation (TAA --> CAC) was detected in two patients. This alteration results in an elongated protein, encoding 107-amino-acid residues (Delta107km23), instead of the wild-type 96-amino-acid form of km23. Furthermore, five missense mutations (T38I, S55G, T56S, I89V, and V90A) were detected in four patients, providing a total alteration rate of 42.1% (8 of 19 cases) in ovarian cancer. No km23 alterations were detected in 15 normal tissues. Such a high alteration rate in ovarian cancer suggests that km23 may play an important role in either TGF-beta resistance or tumor progression in this disease. In keeping with these findings, the functional studies described herein indicate that both the Deltaexon3-km23 and S55G/I89V-km23 mutants displayed a disruption in binding to the dynein intermediate chain in vivo, suggesting a defect in cargo recruitment to the dynein motor complex. In addition, the Deltaexon3-km23 resulted in an inhibition of TGF-beta-dependent transcriptional activation of both the p3TP-lux and activin responsive element reporters. Collectively, our results suggest that km23 alterations found in ovarian cancer patients result in altered dynein motor complex formation and/or aberrant transcriptional regulation by TGF-beta.
ChemInform Abstract: The Coordination Chemistry of Carbon Dioxide and Its Relevance for Catalysis: A Critical Survey (1996)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
T-cell inflamed phenotype gene expression signatures to predict clinical benefit from pembrolizumab across multiple tumor types. (2016)

1536 Background: Two multigene expression signatures previously derived in melanoma patients (pts) and confirmed to be associated with clinical outcome in head and neck and gastric cancer pts, were evaluated in 5 additional cancer types. Methods: We conducted a meta-analysis of the 2 multigene expression signatures in PD-L1+ pts from 5 tumor type cohorts of study KEYNOTE 028. A total of 109 cancer pts (20 anal canal, 18 biliary tract, 22 colorectal, 23 esophageal, 26 ovarian) treated with pembrolizumab on the trial had RNA expression profiling and survival data available; 107 were evaluable for objective response (OR). FFPE-extracted RNA was analyzed on the Nanostring nCounter system and signature scores were calculated as an average of housekeeping normalized expression values of the signature member genes. Statistical regression models tested the association between the predefined signature scores and clinical outcome, adjusted for cancer type for the 2 multigene signatures: 1) IFN-γ and 2) Expanded-immune. Results: Both signature scores showed statistically significant (P< 0.05) association with OR and progression free survival (PFS). Signature scores exhibited the same hallmark pattern seen in head and neck and gastric cancer where the lower end of the signature score distributions had very few patients with OR or prolonged times to progression. The area under the ROC curve for the IFN-γ signature was 0.65 (95% CI: 0.50 - 0.79). Conclusions: This RNA profiling data set from 5 cancers adds to the growing body of evidence that tumor infiltration with activated T-cells is a prerequisite for response to PD-1 checkpoint blockade, and demonstrates that T-cell inflamed gene expression signatures are pan-cancer predictors of clinical benefit from anti-PD-1 treatment; the results suggest that this holds true in pts with PD-L1+ tumors. Such signatures may represent a useful tool for identifying patients who are likely to respond to anti-PD-1 therapy. Clinical trial information: NCT02054806.Signature Nominal 1-sided P valuea OR N = 107 PFS N = 109 IFN-γ (6 gene) 0.010 0.003 Expanded-immune (18 gene) 0.028 0.009 aFrom logistic or Cox regression for OR and PFS, respectively; both regression models included terms adjusting for cancer type.