309 FREQUENT EXPRESSION OF C5AR IN METASTATIC RENAL CELL CARCINOMA (MRCC) AND THE BIOLOGICAL ROLE OF C5A-C5AR SYSTEM IN MRCC (2013)

You have accessJournal of UrologyKidney Cancer: Basic Research (II)1 Apr 2013309 FREQUENT EXPRESSION OF C5AR IN METASTATIC RENAL CELL CARCINOMA (MRCC) AND THE BIOLOGICAL ROLE OF C5A-C5AR SYSTEM IN MRCC Yoshihiro Maeda, Yoshihiro Wada, Yoshiaki Kawano, Ken Kikuchi, Wataru Takahashi, Jiro Honda, Kenichiro Tanoue, Juro Nakanishi, Fumiki Tanigawa, Junji Yatsuda, Takahisa Imamura, and Masatoshi Eto Yoshihiro MaedaYoshihiro Maeda Kumamoto, Japan More articles by this author , Yoshihiro WadaYoshihiro Wada Kumamoto, Japan More articles by this author , Yoshiaki KawanoYoshiaki Kawano Kumamoto, Japan More articles by this author , Ken KikuchiKen Kikuchi Kumamoto, Japan More articles by this author , Wataru TakahashiWataru Takahashi Kumamoto, Japan More articles by this author , Jiro HondaJiro Honda Kumamoto, Japan More articles by this author , Kenichiro TanoueKenichiro Tanoue Kumamoto, Japan More articles by this author , Juro NakanishiJuro Nakanishi Kumamoto, Japan More articles by this author , Fumiki TanigawaFumiki Tanigawa Kumamoto, Japan More articles by this author , Junji YatsudaJunji Yatsuda Kumamoto, Japan More articles by this author , Takahisa ImamuraTakahisa Imamura Kumamoto, Japan More articles by this author , and Masatoshi EtoMasatoshi Eto Kumamoto, Japan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.1693AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Anaphylatoxin C5a is the fragment of complement 5th component, which is a potent chemoattractant for leukocytes. Recent studies showed C5a receptor (C5aR) is aberrantly expressed in human cancers, which plays a crucial role in cancer invasion via enhancing cancer cell motility. In this study, we analyzed expression of C5aR in metastatic and non-metastatic renal cell carcinoma (RCC). The possible role of C5aR in renal cancer cells was also investigated by in vitro analysis. METHODS We retrospectively investigated data from 127 Renal Cell Carcinoma patients who received radical/partial nephrectomy or renal biopsy between 2002 and 2011. C5aR expression in renal cell carcinoma samples were examined by immunohistochemistry using Formalin-Fixed Paraffin-Embedded tissue samples, and correlation between C5aR expression and clinicopathological parameters was analyzed. In addition, murine renal carcinoma cell line RenCa was stably transfected with murine C5aR cDNA, and the effect of C5a stimulation on signal transduction, cellular morphology by western blot and immunofluorescence, respectively. Furthermore, to assess invasion of cancer cell lines in vitro, BioCoat Matrigel invasion chambers were utilized. RESULTS The median (range) age of the patients was 62 (22-87) years. 97 patients (66 men and 31 women) had non-metastatic RCC, whose median (range) age was 63 (22-85) years, and 30 patients (20 men and 10 women) had metastatic RCC (mRCC), whose median (range) age was 59 (30-87) years. Immunohistochemical analysis showed that C5aR expression was observed in 96.7% of RCC cases that was accompanied with metastatic foci, whereas only 50.5% of non-metastatic RCC that expressed C5aR (Fisherüfs Exact Test, p<0.001). There is no correlation between C5aR expression and other clinicopathological parameters. RenCa cells overexpressing mC5aR showed increased ERK activation upon C5a stimulation compared to control cells. F-actin staining using Alexa-conjugated Phalloidin revealed formation of actin stress fiber in mC5aR expressing RenCa cells by C5a stimulation. Matrigel chamber assay demonstrated the invasive ability of RenCa cells overexpressing mC5aR were enhanced significantly by C5a stimulus, compared to control cells. CONCLUSIONS These results suggests that aberrant expression of C5aR may be a pivotal step for cancer cell metastasis and invasion. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e125-e126 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Yoshihiro Maeda Kumamoto, Japan More articles by this author Yoshihiro Wada Kumamoto, Japan More articles by this author Yoshiaki Kawano Kumamoto, Japan More articles by this author Ken Kikuchi Kumamoto, Japan More articles by this author Wataru Takahashi Kumamoto, Japan More articles by this author Jiro Honda Kumamoto, Japan More articles by this author Kenichiro Tanoue Kumamoto, Japan More articles by this author Juro Nakanishi Kumamoto, Japan More articles by this author Fumiki Tanigawa Kumamoto, Japan More articles by this author Junji Yatsuda Kumamoto, Japan More articles by this author Takahisa Imamura Kumamoto, Japan More articles by this author Masatoshi Eto Kumamoto, Japan More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...