gebhart/patrepeval huggingface dataset:
Targeting macrophages and their recruitment in the oral cavity using swellable (+) alpha tocopheryl phosphate nanostructures (2019)

The phosphorylation of (+) alpha tocopherol produces adhesive nanostructures that interact with oral biofilms to restrict their growth. The aim of this work was to understand if these adhesive (+) alpha tocopheryl phosphate (α-TP) nanostructures could also control macrophage responses to the presence of oral bacteria. The (+) α-TP planar bilayer fragments (175 nm ± 21 nm) formed in a Trizma®/ethanol vehicle swelled when exposed to the cell lines (maximum stabilized size = 29 μm). The swelled (+) α-TP aggregates showed selective toxicity towards THP-1 macrophages (LD<sub>50</sub> = 304 μM) compared to human gingival fibroblasts (HGF-1 cells; LD<sub>50</sub> > 5 mM), and they inhibited heat killed bacteria stimulated MCP-1 production in both macrophages (control 57.3 ± 18.1 pg/mL vs (+) α-TP 6.5 ± 3.2 pg/mL) and HGF-1 cells (control 673.5 ± 133 pg/mL vs (+) α-TP - 463.9 ± 68.9 pg/mL).
Lymphomatoid Papulosis Followed by Pityriasis Lichenoides: A Common Pathogenesis? (2011)

Pityriasis lichenoides (PL) and lymphomatoid papulosis (LyP) are uncommon idiopathic eruptions with overlapping clinical and histological features. Although current opinion indicates that PL and LyP are distinct and separate entities, molecular genetic evidence of T-cell clonality in both conditions suggests that an etiopathogenic relationship may exist. We report a patient who was diagnosed with LyP type B in 1985 followed by PL after 11 years. We hypothesize that LyP followed by PL in the same patient reflects differences in the host immune response to a common antigenic stimulus.
Selective Synthesis of TbMn2O5 Nanorods and TbMnO3 Micron Crystals (2006)

Multiferroic rare-earth manganates TbMn2O5 and TbMnO3 were synthesized selectively via a one-pot hydrothermal route. The different morphologies can be obtained by changing the ratio of reactants MnCl2.4H2O and KMnO4. SEM and TEM images showed a high quality for the products that was also confirmed by XPS patterns and Raman spectra.
Conversion of Xylose from Birch Hemicellulose Hydrolysate to 2,3-Butanediol with Bacillus vallismortis (2020)

Biotechnologically produced 2,3-butanediol (2,3-BDO) is a potential starting material for industrial bulk chemicals, such as butadiene or methyl ethyl ketone, which are currently produced from fossil feedstocks. So far, the highest 2,3-BDO concentrations have been obtained with risk class 2 microorganisms and pure glucose as substrate. However, as glucose stays in competition to food and feed industries, a lot of effort has been done in the last years finding efficient alternative substrates. Thereby xylose from hydrolysed wood hemicelluloses is a promising substrate for the production of 2,3-BDO. The risk class 1 microorganism Bacillus vallismortis strain was identified as a very promising 2,3-BDO producer. The strain is able to utilize xylose almost in the same manner as glucose. B. vallismortis is less prone to common inhibiting compounds in lignocellulosic extracts/hydrolysates. When using a concentrated hemicellulose fraction from birch wood hydrolysate, which was produced with ultrafiltration and after which the acetate concentration was reduced, a yield of 0.43 g g−1 was achieved and the xylose consumption and the 2,3-BDO production is basically the same as using pure xylose.
Détection des visages : étude algorithmique et implantation temps réel (2008)

electronic version (8 pp.)
Synthesis and use of 3-amino-4-phenyl-2-piperidones and 4-amino-2-benzazepin-3-ones as conformationally restricted phenylalanine isosteres in renin inhibitors (1992)

The design of P2-P3 conformational restrictions in renin inhibitors by the use of a renin computer graphic model led to the synthesis of inhibitors containing N-Boc, N-acetyl, and N-phthalyl derivatives of 3(S)-amino-4(R,S)-2-piperidones and 4(S)-amino-2-benzazepinones in place of phenylalanine in the control compound N-acetyl-L-phenylalanyl-N-[4(S)-[(butylamino)carbonyl]-1(S)- (cyclohexylmethyl)-2(S)-hydroxy-5-methylhexyl]-L-norleuci namide (32). The piperidone inhibitors were prepared by utilization of the Evans chiral auxilliary to introduce the amino group with enantioselectivity and also to act as a leaving group in an intramolecular cyclization to the piperidone. The most potent inhibitor, 3(S)-(acetylamino)-alpha(S)-butyl-N-[4(S)- [(butylamino)carbonyl]-1(S)-(cyclohexylmethyl)-2(S)-hydroxy-5- methylhexyl]-2-oxo-4(R)-phenyl-1-piperidineacetamide (18, IC50 = 21 nM), was 25-fold less potent than the acyclic control 32. Considerable dependence of potency with the size of the P4 derivative was observed as had been expected based on the presynthetic modeling studies. Attempts to rationalize the observed potencies on the basis of further molecular modeling studies suggested that the loss in inhibitor potency was due to the conformational restrictions distorting the 3S center from the geometry present in the putative extended conformation present when the inhibitor is bound within the renin active site.
EFFECTS OF LEFLUNOMIDE AND OTHER IMMUNOSUPPRESSIVE AGENTS ON T CELL PROLIFERATION IN VITRO1 (1996)

Leflunomide and its active metabolite, A771726, are structurally unrelated to immunosuppressive agents currently under investigation. Previous in vitro studies have revealed that leflunomide primarily inhibits interleukin-2-stimulated T cell proliferation. In the current study, we have extended our previous work and demonstrate that leflunomide prevents T cell progression induced by phytohemagglutinin into the S phase of the cell cycle. To discriminate further the action on T cells of leflunomide from other immunosuppressive agents, we performed kinetic studies where leflunomide was added either after the initiation of mixed lymphocyte cultures (MLC) or after interleukin-2 stimulation of CTLL-4 cell proliferation. These studies revealed that leflunomide acted comparably to rapamycin, but was distinct from brequinar sodium in the MLC, and from cyclosporine and mycophenolic acid in both MLC and CTLL-4. Although previous biochemical studies indicated that leflunomide can inhibit src-family tyrosine kinase activity, more recent studies have suggested that leflunomide can also inhibit pyrimidine synthesis. Our data demonstrate that the ability of leflunomide (25-100 microM) to inhibit MLC and CTLL-4 cell proliferation is partially antagonized by uridine (25-100 microM), and support the hypothesis that leflunomide inhibits pyrimidine synthesis in T cells. Unique molecular mechanisms of immunosuppression suggest that drug combinations may result in synergistic immunosuppression. Our in vitro studies revealed synergistic inhibition of T cell proliferation with the combinations of leflunomide with cyclosporine or with rapamycin. We have extended those studies to quantitate inhibition of MLC by the combinations of leflunomide and brequinar sodium or mycophenolic acid.
Complete genome sequence of a novel extrachromosomal virus-like element identified in planarian Girardia tigrina (2002)

PEVE is the first viral element identified in free-living flatworms. This element differs from all known viruses and viral elements, and comprises two potential helicases that are homologous to proteins from distant viral phyla. PEVE is unevenly distributed in the worm body, and is detected in specific parenchyma cells.
Common inhibitory effects of human anti‐C2 domain inhibitor alloantibodies on factor VIII binding to von Willebrand factor (1995)

Factor VIII (FVIII) inhibitor alloantibodies obtained from seven severe haemophilia A patients were examined for their binding regions and their effects on FVIII binding to von Willebrand factor (vWF). Immunoblotting analysis with a panel of recombinant fragments demonstrated that the binding regions of antibodies in cases 1-5 were contained in the C2 domain of the light chain. Antibodies from cases 1 and 2, which recognized an epitope within residues 2248-2312, completely inhibited FVIII/vWF binding in an ELISA (IC50: 5.0 and 9.0 micrograms/ml, respectively). Antibodies from case 3 recognizing 2170-2312 and case 5 recognizing 2170-2327 also inhibited FVIII/vWF binding (IC50: 110 and 400 micrograms/ml, respectively). Case 4 antibodies recognizing 2218-2307 showed barely detectable inhibition and cases 6 and 7 antibodies recognizing the 44 kD heavy chain, did not inhibit. Our results demonstrate that all anti-C2 alloantibodies with epitopes that extend to the residue 2312 inhibit vWF binding and that an overlap of the inhibitor epitope with residues 2308-2312 is critical for maximal inhibition of vWF binding. Prevention of FVIII/vWF binding appears to be a common property of anti-C2 domain inhibitor alloantibodies.
Hybrid Calixarene/Inorganic Salt/Diiodoperfluorocarbon Supramolecular Assemblies (2006)

1,3-Bis(α-picolyloxy)-p-tert-butylcalix[4]crown-5 in the cone conformation (2), 1,8-diiodoperfluorooctane or 1,6-diiodoperfluorohexane, and potassium iodide ternary mixtures undergo in solution self-sorting and afford crystalline “supramolecular salts”. These hybrid materials consist of supercation [K+ ⊂ 2] and superanion [I–(CF2) n –I…I− …I–(CF2) n –I…I− …] (n = 6,8) components. In the supercations the potassium ion is embedded in the ionophoric pocket created by the heteroatoms present at the lower rim. In the superanions the iodide ions form infinite fluorous polyanionic chains as a result of a self-assembly process which relies on halogen bonding. Both cation encapsulation and anion-perfluorocarbon halogen bonding were detected in solution by 1H and 19F NMR, and in the gas phase by ESI MS.
Identification of cell subpopulations by dual‐color surface immunofluorescence using biotinylated and unlabeled monoclonal antibodies (1988)

A new method of dual-color immunofluorescence is presented for analysis of surface antigen distribution among heterogeneous cell suspensions. It involves flow cytometric analysis of cells stained with a biotinylated first monoclonal antibody and/or with an unlabeled second monoclonal antibody. After addition of streptavidin-phycoerythrin and/or fluoresceinated goat antimouse immunoglobulin antibody, single-cell fluorescence intensities are measured and biparametric graphic representations are obtained, allowing one to determine the percentage of cells stained by each of the monoclonal antibodies or both. The validity of the method was assessed on human peripheral blood mononuclear cells by using three sets of two monoclonal antibodies: CD8 and CD5, CD3 and CD4, CD11 and HLA-DR. The results showed that dual staining did not induce significant quenching or competition between pairs of antibodies. The procedure is simple and sensitive. It requires only minute amounts of monoclonal antibodies. It is readily applicable to the screening of hybridoma supernatants and to the characterization of new antibodies to cell surface antigens with respect to well-defined markers.
Deciphering the complex interplay between microbiota, HPV, inflammation and cancer through cervicovaginal metabolic profiling (2019)

Cervicovaginal metabolic profiles were driven by cancer followed by genital inflammation, HPV infection, and vaginal microbiota. This study provides evidence for metabolite-driven complex host-microbe interactions as hallmarks of cervical cancer with future translational potential. FUND: Flinn Foundation (#1974), Banner Foundation Obstetrics/Gynecology, and NIH NCI (P30-CA023074).
Solvophobic (2016)

In the past, aggregation and self-assembly have been associated principally with micellar and colloidal systems of molecules; however, with the advent of supramolecular chemistry, molecular self-assembly has been opened to a much wider understanding that has facilitated access to a variety of different shapes and sizes, along with the construction of new and fascinating molecular topologies. This document aims at defining more than 150 terms related to aggregation and self-assembly in the particular case of macromolecules. The list is restricted to the most commonly encountered terms.
Dual‐mode analysis of subatomspheric‐pressure CO2 sorption and transport in Kapton H polyimide film (1986)

Abstract Sorption kinetics and equilibria as well as permeabilities and diffusion time lags for CO 2 in Kapton polyimide film have been studied at temperatures from 35 to 55°C and pressures up to 0.78 atm. The sorption/desorption cycles indicate that the diffusivity of CO 2 increases with increasing local penetrant concentration in the polymer. Both the permeability and time lag decrease with increasing upstream CO 2 pressure. All of these results are described well by theoretical expression based on the dual‐mode theory of sorption and transport in glassy polymers.
Mobile UNITY: A Language and Logic for Concurrent Mobile Systems (1997)

Traditionally, a distributed system has been viewed as a collection of fixed computational elements connected by a static network. Prompted by recent advances in wireless communications rechnology, the emerging field of mobile computing is challenging these assumptions by providing mobile hosts with connectivity that may change over time, raising the possibility that hosts may be called upon to operate while only weakly connected to or while completely disconnected from other hosts. We define a concurrent mobile system as one where independently executing coponents may migrate through some space during the course of the computation, and where the pattern of connectivity among the components changes as they move in and out of proximity. Note that this definition is general enough to encompass a system of mobile hosts moving in physical space as well as a system of migrating software agents implemented on a set of possibly non-mobile hosts. In this paper, we present Mobile UNITY, which is a notation for expressing such systems and a logic for reasoning about their temporal properties. Based on the UNITY language of Chandy and Misra, our goal is to find a minimalist model of mobile computation that will allow us to express mobile components in a modular fashion and to reason formally about the possible behaviors of a system composed from mobile components. We also show how the model can contribute to our understanding of mobility by exploring new abstractions for loosely coupled communication and coordination among components.
ChemInform Abstract: Higher‐Carbon Sugars. Part 6. The Synthesis of Some Octose Sugars via the Epoxidation of Unsaturated Precursors. (1987)

Abstract Titanium‐catalyzed asym.
Control with building mass-Part II: Simulation (2006)

Reductions in building peak electrical demand can be achieved by incorporating building-specific models of thermal dynamics into controllers that will implement short-term peak-period curtailment of HVAC capacity or pre-cool the building prior to peak-period cutbacks to increase the magnitude and duration of the load reduction. The same building-specific model can be used to effect energy savings by providing optimal-start control or optimal-pre-cooling control during unoccupied hours. Control logic was developed for pre-cooling with a central HVAC plant equipped with an air-side economizer. Measurement-based estimates were made of chiller performance and internal-gains schedules. The general transient-thermal-response model of the companion paper (Armstrong et al. 2006) was then used to determine building-specific thermal response and estimate the seasonal benefits of several peak-shifting and night-cooling strategies in the office building. Simulations showed a 30% to 60% reduction in seasonal mechanical cooling loads in the office building due to night cooling.
Continuous-wave laser-pumped optical bistability in thermally deposited and molecular-beam-grown ZnSe interference filters (1986)

We observe and compare conditions for cw optical bistability in both thermal-vacuum-deposited and molecular beam grown ZnSe-based interference filters, at He–Ne-laser (633-nm), Kr (647-nm), and Ar-ion (514-nm) wave-lengths. Bistability is observed at 514 and 647 nm and, by inference, should be observable at a number of intervening wavelengths for a single molecular-beam-grown sample. From our theoretical analysis we also demonstrate that observed drift instabilities in filter nonlinear responses correlate with the highest internal irradiance levels.
The importance of shedding of membrane proteins for cytokine biology. (2000)

Most transmembrane proteins are subjected to limited proteolysis by cellular proteases. The recent molecular cloning of the TNF-a converting enzyme (TACE) revealed that this shedding enzyme belongs to a family of metalloproteinases which contain a disintegrin domain (ADAM family). The activity of these proteases seems to be tightly regulated. Mice lacking functional TACE are not viable demonstrating the importance of this enzyme for body homeostasis. This review describes the current knowledge of shedding enzymes, the ADAM protein family, the mechanism of shedding as well as physiological consequences of shedding of cytokines and cytokine receptors for cytokine biology.
A Highly Sensitive, Selective, and Reproducible SERS Sensor for Detection of Trace Metalloids in the Environment (2018)

We have fabricated 3D surface enhanced Raman scattering (SERS) substrates composed of silver-coated gold nanorods (Ag/AuNRs) supported on polycaprolactone (PCL) electrospun fibers for use as sensors of environmental contaminants. The successful fabrication and immobilization of Ag/AuNRs onto PCL fibers further demonstrates the universal nature of our fabrication strategy, benefiting from immobilization based on electrostatic attraction, a nonspecific interaction. For the first time, Ag/AuNRs with different Ag coating thicknesses have been synthesized, characterized by UV–vis spectroscopy and electron microscopy, and assembled onto PCL fibers. The variation of the Ag coating thickness allows the tuning of the surface plasmon resonance (SPR) so as to be in close proximity to the laser wavelength of the probing laser. Using 4-mercaptopyridine (4-Mpy) as a probe molecule, the SERS performance of the Ag/AuNR-based SERS substrate has been investigated. This substrate allowed detection of 4-Mpy at a concentration as low as 10 nM with excellent reproducibility. SERS substrates fabricated with Ag/AuNRs with different Ag coating thicknesses have been compared with substrates using AuNRs. It was found that when fabricated with a Ag coating, the substrates exhibited larger SERS enhancement than that found for substrates fabricated with AuNRs alone. This could be attributed to electron transfer between the gold core and silver shell in a bimetallic rod-shaped nanostructure. In addition, considering the surface chemistry provided by silver coating, we have demonstrated that this new SERS substrate can be used as an environmental sensor for arsenic detection.
A phase I/II study of Pexa-Vec oncolytic virus in combination with immune checkpoint inhibition in refractory colorectal cancer. (2020)

117 Background: The benefit of immune checkpoint inhibition is limited to the small percentage of advanced colorectal cancer (CRC) patients whose tumors present mismatch repair (MMR) gene abnormalities; immunotherapy has not shown benefit in patients with MMR proficient CRC. Oncolytic immunotherapy represents a unique therapeutic platform. This phase I trial tests the safety of the combination of pexastimogene devacirepvec (Pexa-Vec) plus durvalumab (durva) in patients with locally advanced or metastatic CRC. Methods: Eligible patients with advanced proficient mixed match repair (pMMR) CRC received intravenous infusion of Pexa-Vec at dose level 3 x 10 8 plaque forming units (pfu) (DL1) or at 10 9 pfu (DL2) every 2 weeks for 4 doses and durva 1500 mg every 28 days. Response was assessed with CT every 8 weeks. Adverse events were recorded and managed. The primary endpoint included safety, tolerability and feasibility of this combination therapy. Samples of tumor and peripheral blood were collected for assessment of immune parameters. Results: Sixteen patients (6 males and 10 females) enrolled with a median age of 52.1 years (range 39-69) from Dec, 2017 to Oct, 2018. Four patients were treated with Pexa-Vec at DL1 and durva;twelve patients were treated with Pexa-Vec at DL2 and durva.The most common treatment related adverse events (TRAE) included fever 15/16 (94 %), hypotension 12/16 (75 %), chills 12/16 (75%), fatigue 8/16 (50%), sinus tachycardia 7/16 (44%) and rash 6/16 (38%). Grade 3/4 TRAEs were reported in 8/16 (50%)patients; the most common were fever 7/16 (44 %), lymphopenia 2/16 (13%), neutropenia 1/16 (6%) and anemia 1/16 (6%). 14 patients were evaluable for response analysis; one patient 1/14 (7 %) achieved a confirmed partial response (lasting 7.1 months) and continues to receive treatment, while 13 patients had progressive disease. The median progression free survival (PFS) was 2.2 months (95% CI: 2.2-2.3 months) and the median overall survival (OS) was 7.5 months (CI: 4.9-10.1 months). Conclusions: Combination therapy of Pexa-Vec with durva ICI issafe, well tolerated and demonstrates possible activity in patients with advanced pMMR CRC. Clinical trial information: NCT03206073.
Internet Deployment of DPM-based IP Traceback (2007)

In this article, we introduce the Internet deployment guidelines for Deterministic Packet Marking (DPM) - a novel IP traceback method. Unlike other packet marking schemes, DPM cannot be deployed sporadically on the Internet. Therefore, in order to perform the traceback, a structured way of deployment is needed. Related to topology and deployment issues, discussion comparing the features of other full path schemes and ingress packet filtering to those of DPM is also presented.
A new stable CNHC⁁CH⁁CNHCN-heterocyclic dicarbene ligand: its mono- and dinuclear Ir(i) and Ir(i)–Rh(i) complexes (2009)

The ligand [(1,3-phenylene)bis(methylene)]bis(1-ethyl-imidazol-2-ylidene) ( 2) (abbreviated as EtCNHC--CH--CNHC with CH = central aromatic ring, -- = CH2 spacer) has been synthesised, and isolated, by deprotonation of [(1,3-phenylene)bis(methylene)]bis(1-ethyl-imidazolium) dichloride, Et(CHimid.--CH--CHimid.)Cl2 ( 1) with LiN(SiMe3)2. This new, remarkably stable NHC dicarbene ligand is quantitatively protonated with HBr to form [(1,3-phenylene)bis(methylene)]bis(1-ethyl-imidazolium) dibromide Et(CHimid.--CH--CHimid.)Br2 ( 3) and reprotonated slowly enough in MeOH to allow observation of the monoprotonated intermediate 4. Dicarbene 2 reacts with S8 to give the dithione compound [(1,3-phenylene)bis(methylene)]bis(1-ethyl-imidazol-2-thione) Et[(SC)--CH--(CS)] ( 6). Reaction of 2 with [IrCl(CO)(PPh3)2] and [Ir(mu-Cl)(cod)]2 yielded the mononuclear [Ir(CO)(PPh3)2Et(CHimid.--CH--CNHC)(PF6)](PF6) ( 8) and [IrCl(cod)Et(CHimid.--CH--CNHC)](PF6) ( 10) complexes, respectively, and the dinuclear complexes [{Ir(CO)(PPh3)2}2Et(mu-CNHC--CH--CNHC)](PF6)2 ( 7) and [{IrCl(cod)}2Et(mu-CNHC--CH--CNHC)] ( 9), respectively, in which 2 acts as a bridging, non-pincer type ligand. Only one other Ir(I) complex has been reported before containing a CNHC--CH--CNHC ligand. The structures of 6 and 9 were determined by X-ray diffraction and depict different conformations of the (1,3-phenylene)bis(methylene) (or xylylene) moiety. The mononuclear Ir complex 9 reacted smoothly with [Rh(mu-Cl)(cod)]2 and Cs2CO3 to generate a rare example of a heteronuclear NHC complex, [IrRhCl2(cod)2Et(mu-CNHC--CH--CNHC)] ( 11).
OptiVeo: A Vision-Based Platform for Driving Assistance (2006)

&lt;div class="htmlview paragraph"&gt;Most of the Driving Assistance functions require sensors that are looking ahead of the vehicle. Among these sensors, cameras, already providing Lane Departure Warning and Night Vision, will play a major role. Since it won't be possible to multiply the number of sensors located behind the windscreen, it is then crucial to perform with cameras as many detections as possible. We describe in this paper some algorithmic methods for performing camera based tunnel and fog detection.&lt;/div&gt;