gebhart/patrepeval huggingface dataset:
Maggot Therapy for Treating Pressure Ulcers in Spinal Cord Injury Patients (1995)

For centuries, maggot therapy (MT) has been recognized as an aid to wound healing. By including live blowfly larvae in wound dressings, earlier physicians noted thorough debridement which hastened wound healing. We initiated a prospective controlled study to evaluate the utility of maggot therapy for treating pressure ulcers in spinal cord injury patients in the modern era. Eight of our patients received MT after a baseline assessment of healing under conventional therapy (defined as any therapy prescribed by the patient's primary care team). Surface area, tissue quality and healing rates were monitored weekly. MT debrided most of the necrotic wounds within one week, which was more rapid than all other non-surgical methods. Wound healing was more rapid during MT than during antecedent conventional therapy (p = 0.01). No complications were seen. We have demonstrated that MT can be beneficial in the treatment of pressure ulcers in persons with spinal cord injuries. MT was significantly more effective and efficient than the current, conventional treatment alternatives being used. MT was also safe, simple and inexpensive. MT can be a valuable modality in the treatment of pressure ulcers.
Abstract 310: Thrombospondin 2 Protects Against Uncontrolled Cardiac Inflammation and Injury During Viral Myocarditis (2008)

Background-The matricellular protein thrombospondin 2 (TSP2), a secreted extracellular matrix (ECM) glycoprotein, plays a crucial role in controlling cell-matrix interaction in tissue repair and cancer biology. Our previous data revealed that increased TSP2 reinforces the matrix and protects the against heart failure during hypertension. The present study investigated whether TSP2 may protects against exaggerated cardiac inflammation, injury and dysfunction during viral myocarditis. Methods and Results-Therefore, mortality, cardiac inflammation and function were investigated in TSP2-null (KO) and wild type (WT) mice, and after adeno-associated virus (AAV)-9 mediated TSP-2 overexpression in WT, in a mouse model of coxsackievirus B3 (CVB3)-induced (106 CCID50 ip CBV3, 14 days) myocarditis. Absence of TSP2 resulted in an increased mortality in TSP2-null as compared with WT mice in both viral myocarditis (KO: 37,5% vs WT: 0%; n=8 per group). Histological analysis revealed increased cardiac inflammation (60 % increase of CD3-positive T-lymphocytes), necrosis and injury in TSP2-null compared to WT mice at 14 days of viral myocarditis. Ultrastructural analysis showed increased inflammation, matrix disruption and myocyte injury in absence of TSP2, together resulting in depressed systolic function (% FS; 40.1 ± 2.3 in WT vs. 24.6 ± 1.7 in KO mice, P&lt;0.05) and increased cardiac dilatation (end-diastolic dimensions, mm; 2.6 ± 0.18 in WT vs. 3.6 ± 0.06 in KO mice, P&lt;0.05) at 14 days at echocardiography. In concordance, AAV9-gene transfer of TSP2, with 50 % increase in cardiac TSP-2 expression, significantly reduced cardiac inflammation (60% reduction) and injury at 14 days as compared to control AAV9-GFP. Conclusion-Together, these data clearly point towards a novel protective role for TSP2 against uncontrolled inflammation, myocardial injury and dysfunction in acute viral myocarditis.
Electrochemical Characteristics of a Novel Pyridinium Salt as a Candidate Drug Molecule and Its Interaction with DNA (2020)

Abstract In this article, for the first time, the electrochemical properties of a novel pyridine derivative, 4‐(2‐(2‐hydroxybenzylidene) hydrazinyl)‐1‐(3‐phenylpropyl) pyridinium bromide (abbreviated as 4‐Pyri), and its interaction with double stranded DNA (dsDNA) was investigated. The interaction between candidate drug molecule (4‐Pyri) and dsDNA was analyzed by examining 4‐Pyri (+0.6 V and +0.8 V) and guanine (+1.0 V) oxidation signal changes with Differential Pulse Voltammetry (DPV) and Cyclic Voltammetry (CV). Electrochemical Impedance Spectroscopy (EIS) was used to show the resistance changes before and after the interaction between 4‐Pyri and dsDNA. We showed that after the interaction with 4‐Pyri, the oxidation currents of guanine decreased dramatically, whereas the intrinsic oxidation currents of 4‐Pyri dramatically increased. 4‐Pyri oxidation current differences before and after the interaction with dsDNA enabled us to determine such interaction separately from guanine oxidation signals. In addition, resistance differences were observed at before and after the interaction with each other that confirmed the possible interaction. In addition, toxicity effect (S%) value, which is an important parameter for electrochemical studies indicated 4‐Pyri's toxicity to dsDNA. Our results demonstrated that 4‐Pyri interacts with dsDNA, and could be used as a potential candidate drug molecule due to its remarkable impact on dsDNA.
Prostate cancer epidemiology (2002)

Prikazan je pregled faktora rizika za rak prostate te incidencija i mortalitet od raka prostate u Hrvatskoj i u svijetu.
Chemical, physical-chemical, and immunological properties of papain-solubilized human transplantation antigens (1979)

Papain-solubilized HLA-A, -B, and -C antigens have been isolated from cadaveric spleens. The isolated material was homogeneous and comprised subunits with the apparent molecular weights 33,000 and 12,000. Amino acid analyses of a mixture of HLA antigen heavy chains obtained from a great number of spleens with different HLA antigen phenotypes revealed a composition that is very similar to that of individual HLA-A and -B antigens. Likewise, the NH2-terminal 30 residues of the HLA-antigen heavy chain mixture were virtually identical with that recorded for individual specificities. The circular dichroism spectra for the isolated HLA antigens and for free beta2-microglobulin revealed similarities with spectra recorded for immunoglobulin chains and domains. The HLA-antigen heavy chain may contain an appreciable amount of beta structure. Antibodies raised against free beta2-microglobulin react better with beta2-microglobulin in free form than when bound to the HLA-A, -B, and -C antigen heavy chains. This is due to the fact that free beta2-microglobulin can bind a maximum of four Fab fragments simultaneously, whereas the HLA-antigen-associated beta2-microglobulin can bind only two Fab fragments without dissociating from the heavy HLA-antigen subunit.
Ergonomic assessment of the surgeon's physical workload during standard and robotic assisted laparoscopic procedures (2013)

Robot-assisted laparoscopy is a less physically stressful surgical technique than standard laparoscopy.
Uniformity of N‐fertiliser spreading and risk of ground water contamination (2002)

Abstract The uniformity of fertiliser spreading mainly depends on the evenness of the transversal distribution. During the last few years, more than 300 fertiliser spreaders have been tested on farms. The results have shown big differences between machines (coefficient of variation, CV, between 5 and more than 50%). The spreading homogeneity is linked to spreader features, fertiliser characteristics and user skills. The contamination of ground water by nitrogen depends on the locally applied quantity of fertiliser. Using farm spreading patterns, a simulation of the risk of contamination has been established, based on the increase of typical spreading errors. The risk of ground water contamination is assessed by using curves of residual nitrogen observed under field conditions in Belgium. It is thus possible to determine an acceptable level of fertiliser spreading heterogeneity. Copyright © 2002 John Wiley &amp; Sons, Ltd.
CD200Fc(Gly)6TGFβ suppresses transplant rejection and MLCs in vitro (49.15) (2010)

Abstract CD200 suppresses graft rejection after engagement of receptors (CD200R), via induction of Treg and production of TGFβ. We asked if a hybrid molecule linking a soluble form of CD200, CD200Fc, to murine TGFβ through a (Gly)6 linker would produce superior suppression to either CD200FC or TGFβ alone, or in combination. MLC cultures used BL/6 responder cells and irradiated BALB/c DCs, in the presence/absence of varying doses of CD200Fc or TGFβ, alone or in combination, or CD200Fc(Gly)6TGFβ. CD200Fc(Gly)6TGFβ produced suppression at 20-100-fold lower concentrations than all other reagents tested alone or together. When used in vivo to suppress rejection of BALB/c skin grafts in C57BL/6 mice, CD200Fc(Gly)6TGFβ also produced immunosuppression at 100-fold lower concentrations. Further studies suggest the mechanism(s) by which this novel molecule produces suppression involves increased numbers of (inducible) Foxp3+ Treg which cause antigen-specific suppression in MLCs in vitro. Using cells with targeted deletion of the main receptor for CD200 (CD200R1), and/or treated with lentiviral particles encoding shRNAs specific for TGFβRII, we showed that suppression depended on responder T cells with a functional TGFβRII, and DCs expressing CD200R1. Augmented inhibition of suppression by CD200Fc(Gly)6TGFβ using anti-CD200R2 in cultures from CD200R1 knockout mice confirmed a role for CD200R2 in suppression. We conclude that CD200Fc(Gly)6TGFβ may have clinical utility in vivo, or ex vivo.
Paosphorothioate Analogues of Inositol Phosphates (1990)

Abstract Novel analogues of the intracellular second messenger D-myo-inositol 1,4,5-trisphosphate, which possess phosphorothioate groups in place of phosphate groups have been synthesized. They exhibit unusual biological properties which will be of considerable application in understanding the phosphoinositide cycle.
Val50Met Hereditary Transthyretin Amyloidosis: Not Just a Medical Problem, but a Psychosocial Burden (2020)

Abstract Hereditary transthyretin (TTR) amyloidosis (ATTRv) is a heterogeneous disease with a clinical presentation that varies according to geographical area and TTR mutation. The symptoms of Val50Met-ATTRv are mainly neuropathic and progress to complete disability and death in most untreated patients within 10 to 15 years of diagnosis. The neurological effects may also be accompanied by gastrointestinal impairment, cardiomyopathy, nephropathy and/or ocular deposition. The disease is thus associated with a high degree of patient disability. Accordingly, we aimed to describe the psychosocial burden associated with ATTRv in a group of patients, asymptomatic Val50Met carriers, relatives and caregivers in the endemic focus of the disease in Majorca via a survey addressing various aspects related to psychosocial burden.Our study revealed that, although most of the population studied had had prior contact with ATTRv through affected relatives, there was still a lack of information regarding disease diagnosis. Fear of the genetic test result and psychological issues were common in our population. Moreover, the disease had a stronger impact on the daily life of our patients than that of our asymptomatic carriers. Autonomic symptoms were the main source of burden for relatives and caregivers. In conclusion, our survey results show high psychosocial burden associated with Val50Met-ATTRv in our area.
Concentration, biosynthesis and degradation of collagen in idiopathic pulmonary fibrosis. (1986)

Despite several studies both in vitro and in vivo, the pathogenesis of pulmonary fibrosis is unclear and some findings related to the biochemistry of collagen are controversial. Collagen metabolism was studied in 11 patients with idiopathic pulmonary fibrosis and in six control subjects. There was an increase in collagen concentration (mean 327 (SD 76) compared with control values of 185 (18) micrograms/mg dry weight, p less than 0.001), normal values for biosynthesis (mean 2.2% (0.8%) v 2.08% (0.5%), and a noteworthy decrease in collagenolytic activity (mean 0.07 (0.04) v 0.23 (0.04) micrograms of collagen degraded per mg of collagen incubated, p less than 0.001). These results suggest that an alteration in enzymatic breakdown of collagen plays an important role in the maintenance and progression of interstitial fibrosis in this disease.
Analyzing the effect of a metamaterial surface on electric and magnetic dipole emissions using Green's function and spatial harmonic analysis techniques (2010)

An approach for calculating the fields radiated from either electric or magnetic dipoles placed inside or at the surface of a multilayer structure arranged of arbitrary elemental materials, including metamaterials, is presented. A combination of Green's function technique and Spatial Harmonic Analysis method is used. The electromagnetic behavior of the dipole sources in the vicinity of the metamaterial surface is studied. This approach is applicable to the important case when the near field of fluorescent molecules is coupled to a multilayer single-periodic or bi-periodic nanostructure.
Gerenciando sem recorrer a soluções paliativas: um programa totalmente integrado para criar e manter o sucesso organizacional (1992)

Made available in DSpace on 2014-07-16T17:31:15Z (GMT). No. of bitstreams: 0 Previous issue date: 1992-10-01
Wildfire Monitoring with Uneven Importance Using Multiple Unmanned Aircraft Systems (2019)

Unmanned Aircraft Systems (UASs) have great potentials in wildfire monitoring to provide real time data to support better wildfire management. This paper focuses on the problem of multi-UAS coordination in wildfire monitoring. To effectively deal with the heterogeneous behavior of wildfire spread, we develop an importance-based coordination method that takes into consideration the different levels of attention needed for monitoring different regions of a fire perimeter. The developed method is compared with a rendezvous-based coordination method and a circling-based coordination method and is evaluated in a wildfire spread simulation environment. Experiment results show that the importance-based coordination is able to direct UASs to visit the active regions of a wildfire more frequently while monitoring the entire fire perimeter.
Electrical Properties of Atomic-Layer-Deposited La<sub>2</sub>O<sub>3</sub>/Thermal-Nitrided SiO<sub>2</sub> Stacking Dielectric on 4H-SiC(0001) (2007)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.
Hardware Components in Optical Lithography (2021)

An optical lithography imaging system includes the following key components: a light source that provides the exposure photons with the desired energy spectrum; an illuminator that collects light from the source, adjusts its coherence and incident angles, and delivers light to the mask uniformly; a mask containing the circuit image to be replicated to the photoresist; an imaging lens of the desired NA and field size to reproduce the mask image in the resist by exposure; the resist layer coated on the wafer; the thin-film stack to be delineated by the pattern-transfer process or implantation; the wafer held by a chuck on a wafer stage that can be moved for alignment and field stepping; and alignment-viewing devices to guide the aligning movement of the stage. These components are described consecutively in the following sections.
Strength and Chemical Stability Due to Aging of Two Bone Void Filler Materials (2007)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.
Salt and Blood Pressure: New Insight from Human Genetic Studies (2002)

Heart disease and stroke are the number one and number three leading causes of death in the United States, accounting for a third of all deaths annually. Epidemiologicstudies have established a number of risk factors for thesediseases, including hypertension, high cholesterol, diabetes mellitus, and smoking. Prospective randomized trials of blood pressure and cholesterol reduction and smoking cessation have established the causal relationship ofthese risk factors to disease, because modifying these parameters prevents adverse clinical outcomes, includingdeath...
Metrology variability and its impact in process modeling (2013)

In electron proximity effects correction (PEC), the quality of a correction is highly dependent on the quality of the model used to compute the effects. Therefore it is of primary importance to have a reliable methodology to extract the parameters and assess the quality of a model. Usually, model calibration procedures consist of one or more cycles of exposure and measurements on the calibration stage. The process and metrology variability may play a key role in the quality of the final model and, hence, of the PEC result. Therefore, it is important to determine at which level these variations may impact a calibration procedure and how a calibration design may be implemented in order to enable more robustness to the resulting model. In this work, metrology variability was evaluated by measuring the same wafer using two different CD-SEM tools. The information coming from these analyses was used as reference to a variation induced calibration test using synthetic data. By inserting variability in synthetic data it was possible to evaluate its impact on the resulting parameter values and in the final model error evaluation.
Natural killer cells efficiently target multiple myeloma clonogenic tumor cells (2021)

The multiple myeloma (MM) landscape has changed in the last few years, but most patients eventually relapse because current treatment modalities do not target clonogenic stem cells, which are drug-resistant and can self-renew. We hypothesized that side population (SP) cells represent myeloma clonogenic stem cells and, searching for new treatment strategies, analyzed the anti-myeloma activity of natural killer (NK) cells against clonogenic cells. Activated and expanded NK cells (NKAE) products were obtained by co-culturing NK cells from MM patients with K562-mb15-41BBL cell line and characterized by flow cytometry. Functional experiments against MM cells were performed by Eu-TDA release assays and methylcellulose clonogenic assays. Side population was detected by Dye Cycle Violet labeling and then characterized by flow cytometry and RNA-Seq. Self-renewal capacity was tested by clonogenic assays. Sorting of both kind of cells was performed for time-lapse microscopy experiments. SP cells exhibited self-renewal potential and overexpressed genes involved in stem cell metabolism. NK cells from MM patients exhibited dysregulation and had lower anti-tumor potential against clonogenic cells than healthy donors' NK cells. Patients' NK cells were activated and expanded. These cells recovered cytotoxic activity and could specifically destroy clonogenic myeloma cells. They also had a highly cytotoxic phenotype expressing NKG2D receptor. Blocking NKG2D receptor decreased NK cell activity against clonogenic myeloma cells, and activated NK cells were able to destroy SP cells, which expressed NKG2D ligands. SP cells could represent the stem cell compartment in MM. This is the first report describing NK cell activity against myeloma clonogenic cells.
Volume plasmon polaritons and subwavelength interference in a hyperbolic medium (2013)

Experiments demonstrate that inside strongly anisotropic materials with hyperbolic dispersion, light diffracted from double slits propagates as volume plasmon polaritons and form subwavelength interference peaks that can be used in subwavelength photolithography and probes.
A magneto-DNA nanoparticle system for the rapid and sensitive diagnosis of enteric fever (2016)

There is currently no widely available optimal assay for diagnosing patients with enteric fever. Here we present a novel assay designed to detect amplified Salmonella nucleic acid (mRNA) using magneto-DNA probes and a miniaturized nuclear magnetic resonance device. We designed primers for genes specific to S. Typhi, S. Paratyphi A, and genes conserved among Salmonella enterica spp. and utilized strongly magnetized nanoparticles to enhance the detection signal. Blood samples spiked with in vitro grown S. Typhi, S. Paratyphi A, S. Typhimurium, and E. coli were used to confirm the specificity of each probe-set, and serial 10-fold dilutions were used to determine the limit of the detection of the assay, 0.01-1.0 CFU/ml. For proof of principle, we applied our assay to 0.5 mL blood samples from 5 patients with culture-confirmed enteric fever from Bangladesh in comparison to 3 healthy controls. We were able to detect amplified target cDNA in all 5 cases of enteric fever; no detectable signal was seen in the healthy controls. Our results suggest that a magneto-DNA nanoparticle system, with an assay time from blood collection of 3.5 hours, may be a promising platform for the rapid and culture-free diagnosis of enteric fever and non-typhoidal Salmonella bacteremia.
In Silico Processing of the Complete CRISPR-Cas Spacer Space for Identification of PAM Sequences (2018)

Abstract Despite extensive exploration of the diversity of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) systems, biological applications have been mostly confined to Class 2 systems, specifically the Cas9 and Cas12 (formerly Cpf1) single effector proteins. A key limitation of exploring and utilizing other CRISPR-Cas systems with unique functionalities, particularly Class I types and their multi-protein effector complex, is the knowledge of the system’s protospacer adjacent motif (PAM) sequence identity. In this work, we developed a systematic pipeline, named CASPERpam, that enables us to comprehensively assess the PAM sequences of all the available CRISPR-Cas systems in the NCBI database of bacterial genomes. The CASPERpam analysis revealed that within the 30,389 assemblies previously screen for CRISPR arrays, there exists 26,364 spacers that match somewhere in the viral, bacterial, and plasmid databases of NCBI, using the constraints of 95% sequence identity and 95% sequence coverage for blast hits. When grouping these results by species, we were able to identify putative PAM sequences for 1,049 among 1,493 unique species. The remaining species either have insufficient data or an undetermined result from the analysis. Finally, we were able to infer certain design principles that are relevant for understanding PAM diversity and a baseline for further experimental studies including PAM assays. We envision CASPERpam is a useful bioinformatic tool for understanding and harnessing the diversity of CRISPR systems.
Theoretical derivation of antenna parameters for thin-wire nanoloops (2016)

The development of nanoantennas is mostly done through time-consuming computer simulations. Nanoloop antennas, working in the terahertz, infrared and optical regimes, have used in a variety of applications, including as solar cells or optical sensors. In this paper we will present a theoretical analysis of thin-wire nanoloops, which leads to closed-form expressions to derive key antenna parameters: radiated electric fields, radiated power, radiation resistance, directivity and gain. Results for gold thin-wire nanoloops are validated with full-wave simulations, and the presentation includes a comparison with classical nanodimensional PEC loops, emphasizing the differences and similarities from a physical point of view. While full-wave simulations can take on the order of hours, the analytical expressions can be evaluated on the order of seconds, thus allowing a wider assessment of the usefulness of these nanoantennas in practical applications.