gebhart/patrepeval huggingface dataset:
Towards software support for situation-specific cross-organizational design thinking processes (2022)

To deal with uncertain user needs and the development of cross-organizational solutions, Design Thinking (DT) processes are one general problem-solving approach. However, DTs actual implementation within organizations depends highly on the situation (e.g., goal to achieve) which affects the conducted process itself (e.g., different tasks to conduct), the used places (e.g., different visualization on whiteboards), and the involved people (e.g., different stakeholders for specific tasks). Currently, the situation needs to be covered manually by the DT moderator during the process construction and is insufficiently addressed by existing software tools during execution. Therefore, we propose a design science study to develop abstract design knowledge and a specific software tool for supporting situation-specific DT processes based on the application domain on cross-organizational development of business models for business ecosystems. Within this workshop paper, we provide preliminary design principles together with a software tool based on the practical experience in existing research projects.
Phages infecting Faecalibacterium prausnitzii belong to novel viral genera that help to decipher intestinal viromes (2018)

Since IBD patients generally have less F. prausnitzii in their microbiota than healthy controls, the higher prevalence or abundance of some of its phages may indicate that they are activated during disease. This in turn suggests that phages could trigger or aggravate F. prausnitzii depletion in patients. Our results show that prophage detection in sequenced strains of the microbiota can usefully complement viral metagenomic studies.
EPHRINA2 RECEPTOR IN HUMAN GLIOBLASTOMA CANCER STEM CELLS AND IDENTIFICATION OF NEW, PUTATIVE THERAPEUTIC TARGETS (2014)

BACKGROUND: Human glioblastomas (hGBMs) have been shown to embody a relatively small subset of cells which bear the defining features of somatic stem cells and the ability to establish, expand and perpetuate these tumors. They are defined cancer stem cell or tumor propagating cells (TPCs). This has caused a paradigmatic shift in the way we interpret hGBM physiology, for it identifies TPCs as a major culprit to be tackled for the development of novel therapeutics. Hence, we inferred that the study of regulatory mechanisms of normal neurogenesis may lead us to identify specific inhibitors of TPCs. METHODS: TPCs from human GBMs were used to determine the role of a neural stem cell regulator, the EphA2 receptor, in modulating self-renewal and tumorigenicity. We used a combined series of in vitro experiments which include primary tissue cultures, FACS analysis, next generation sequencing screening and in vivo, orthotopic xenografting experiments entailing the intracerebral delivery of putative therapeutic agents. RESULTS: We determined the over-expression of the Ephrin receptor type A2 (EphA2) in hGBM TPCs. EphA2 upregulation causally underlies self-renewal and expansion of the TPCs pool. Most important, both the EphA2 cognate ligand ephrinA1-Fc causes EphA2 downregulation in TPCs and suppress TPC self-renewal and intracranial tumorigenicity. This points to EphA2 as a key effector in TPCs self-renewal and tumorigenicity. Notably, intracranial administration of ephrinA1-Fc causes both down-regulation of EphA2 in hGBMs orthotopic xenografts pre-established in mice, whose growth and expansion throughout the brain parenchyma is significantly hindered in the absence of cytotoxic effects. CONCLUSIONS: We identify TPCs as a major target of EphA2 overexpression in hGBM, that drives their self-renewal and tumorigenicity. We propose TPCs as a specific cellular target in which enforced down-regulation of a molecular target such as EphA2 may be exploited for therapeutic purposes, under therapy-like scenarios. In addition cytofluorimetric sorting into EphA2-high and EphA2-low populations demonstrates that EphA2 expression correlates with the size of the hGBM, TPC pool and that this can be used to purify TPCs. We have exploited this technique in a comparative next generation sequencing approach, in which the transcriptomic profile of purified TPCs was compared to that of their non-tumorigenic siblings from the same FACS preparation. We identified a series of putative TPC critical regulators involved in self-renewal and invasion. This investigation is being deepened and the most cogent findings on this subject will be discussed in our presentation. SECONDARY CATEGORY: Preclinical Experimental Therapeutics.
Dissecting human T cell responses against Bordetella species. (1988)

To identify the minimal structures that may be important for the creation of a synthetic and/or recombinant vaccine against whooping cough, human T cell clones were obtained against Bordetella antigens. Cloned peripheral blood T lymphocytes from an immune donor were grown in IL-2 and tested for proliferation in response to inactivated Bordetella species (B. pertussis, B. parapertussis, and B. bronchiseptica) and mutants deficient for the expression of virulence-associated antigens. All the T cell clones obtained were CD4+8- and recognized specifically the Bordetella antigens when presented by autologous B cells. On the basis of the responsiveness to the whole inactivated bacteria, it was possible to cluster the 12 clones obtained into four groups with the following specificity: (1) filamentous hemagglutinin (FHA); (2) B. pertussis-specific antigens; (3) virulence-associated Bordetella-specific antigens; and (4) nonvirulence-associated Bordetella-specific antigens. Using two new B. pertussis deletion mutants, clone 6 (representative of cluster 1) was found to recognize the COOH terminus of FHA. Furthermore, three out of four clones of cluster 3 were specifically stimulated by the soluble 69-kD protein from the outer membrane of B. pertussis. Surprisingly, none of the twelve clones obtained by stimulation in vitro with whole inactivated bacteria recognized pertussis toxin (PT), which is believed to be the most important protein to be included in an acellular vaccine. However, when a new generation of clones was obtained using soluble PT as the in vitro stimulus, it was observed that 11 clones of this group recognized this antigen. Thus, PT does not seem to be the most representative antigen on the whole inactivated bacteria, although T cell memory against PT exists in a donor who had the disease several years ago.
Mid-infrared Plasmonics for Ultra-sensitive Chemical Fingerprinting and Real-time, in-situ Spectroscopy (2013)

We demonstrate a plasmonic platform performing real-time, in-situ infrared absorption spectroscopy on biomolecule binding interactions. Leveraging nano-antennas for near-field enhancement and to direct radiation, our approach enables an ultrasensitive compact chip based biosensor technology.
On the value of synchronous downlink MIMO-OFDMA systems with linear equalizers (2008)

Cellular radio systems are often limited due to the presence of cochannel interference. Basically, radio systems may be operated by utilizing asynchronous or synchronous downlink transmission from all base stations in the system, where synchronization between terminals and their serving base station is mandatory in both concepts. We provide a comparison between the theoretical achievable spectral efficiency in an orthogonal frequency division multiple access systems using different linear equalizers and their resulting performance taking estimation errors into account. It is shown, that the choice of the appropriate receiver depends on the degree of synchronization in the system. We demonstrate that a 3G long term evolution radio system may achieve higher spectral efficiency thanks to interference suppression if fully synchronized data transmission from all base stations is introduced.
Molecular cloning and primary structure of Thermoactinomyces vulgaris carboxypeptidase T A metalloenzyme endowed with dual substrate specificity (1991)

A gene coding for an extracellular Zn-carboxypeptidase of Thermoactinomyces vulgaris has been cloned and sequenced (EMBL X56901). This enzyme named carboxypeptidase T reveals simultaneously both types of substrate specificity characteristic of mammalian carboxypeptidases A and B. The carboxypeptidase T gene is primarily expressed in E. coli as a non-active preproenzyme with an additional 98 amino acid residues at the N-terminus. Primary structure alignment of mature carboxypeptidase T and mammalian metallocarboxypeptidases demonstrated 25-30% overall identity but a full preservation of presumed catalytically important residues. These observations imply a basic uniformity of the general catalytic mechanism for enzymes of that class produced by evolutionarily remote organisms.
A 28.6dBm 65nm Class-E PA with Envelope Restoration by Pulse-Width and Pulse-Position Modulation (2008)

This paper introduces a Class-E PA that overcomes the various limitations using pulse-width and pulse-position modulation (PWPM).
Laser fluorescence analysis of chromatograms. Subnanogram detection of aflatoxins (1975)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLaser fluorescence analysis of chromatograms. Subnanogram detection of aflatoxinsMichael R. Berman and Richard N. ZareCite this: Anal. Chem. 1975, 47, 7, 1200–1201Publication Date (Print):June 1, 1975Publication History Published online1 May 2002Published inissue 1 June 1975https://pubs.acs.org/doi/10.1021/ac60357a065https://doi.org/10.1021/ac60357a065research-articleACS PublicationsRequest reuse permissionsArticle Views98Altmetric-Citations20LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Pharmacokinetics of cannabidiol in a randomized crossover trial in senior horses (2022)

Pharmacokinetics and bioavailability of CBD in senior horses were determined, and there were no adverse effects of administering either the oral or IV dose of CBD evaluated.
Divalent Lanthanide Metallocene Complexes with a Linear Coordination Geometry and Pronounced 6s–5d Orbital Mixing (2022)

A small but growing number of molecular compounds have been isolated featuring divalent lanthanides with 4f<sup><i>n</i></sup>5d<sub><i>z</i><sup>2</sup></sub><sup>1</sup> electron configurations. While the majority of these possess trigonal coordination geometries, we previously reported the first examples of linear divalent metallocenes Ln(Cp<sup>iPr5</sup>)<sub>2</sub> (Ln = Tb, Dy; Cp<sup>iPr5</sup> = pentaisopropylcyclopentadienyl). Here, we report the synthesis and characterization of the remainder of the Ln(Cp<sup>iPr5</sup>)<sub>2</sub> (<b>1-Ln</b>) series (including Y and excluding Pm). The compounds can be synthesized through salt metathesis of LnI<sub>3</sub> and NaCp<sup>iPr5</sup> followed by potassium graphite reduction for Ln = Y, La, Ce, Pr, Nd, Gd, Ho, and Er, by <i>in situ</i> reduction during salt metathesis of LnI<sub>3</sub> and NaCp<sup>iPr5</sup> for Ln = Tm and Lu, or through salt metathesis from LnI<sub>2</sub> and NaCp<sup>iPr5</sup> for Ln = Sm, Eu, and Yb. Single crystal X-ray diffraction analyses of <b>1-Ln</b> confirm a linear coordination geometry with pseudo-<i>D</i><sub>5<i>d</i></sub> symmetry for the entire series. Structural and ultraviolet-visible spectroscopy data support a 4f<sup><i>n</i>+1</sup> electron configuration for Ln<sup>2+</sup> = Sm, Eu, Tm, and Yb and a 4f<sup><i>n</i></sup>5d<sub><i>z</i><sup>2</sup></sub><sup>1</sup> configuration for the other lanthanides ([Kr]4d<sub><i>z</i>2</sub><sup>1</sup> for Y<sup>2+</sup>). Characterization of <b>1-Ln</b> (Ln = Y, La) using electron paramagnetic resonance spectroscopy reveals significant s-d orbital mixing in the highest occupied molecular orbital and hyperfine coupling constants that are the largest reported to date for divalent compounds of yttrium and lanthanum. Evaluation of the room temperature magnetic susceptibilities of <b>1-Ln</b> and comparison with values previously reported for trigonal Ln<sup>2+</sup> compounds suggests that the more pronounced 6s-5d mixing may be associated with weaker 4f-5d spin coupling.
Enabling Hybrid Bonding on Intel Process (2021)

In this paper, we holistically discuss the recent design, wafer fabrication and die assembly changes needed to enable hybrid bonding interconnect (HBI) on Intel process. HBI enables orders of magnitude improved interconnect density compared to solder which opens the doors to many new 3D packaging architectures. Starting with the design aspect, we show the physical interconnect changes that results in much lower parasitics. We also show the impacts on the top metal layer passives and power delivery and how to minimize these impacts through specific design changes. Afterwards, the wafer fabrication process changes are shown such as the fabrication of the bonding metal layers without causing excessive wafer warpage and the optimization of the chemical mechanical polishing process to enable smooth and flat surface dielectric and controlled-recess bonding pads. The assembly process changes are also summarized with special emphasis on the need for extremely clean die surface, high accuracy die placement and maximized test coverage. Finally, we show the design, fabrication, assembly and test results of active and passive test chips utilizing Intel advanced node process.
MOFs as multifunctional catalysts: One-pot synthesis of menthol from citronellal over a bifunctional MIL-101 catalyst (2012)

A bifunctional MOF catalyst containing coordinatively unsaturated Cr(3+) sites and palladium nanoparticles (Pd@MIL-101) has been used for the cyclization of citronellal to isopulegol and for the one-pot tandem isomerization/hydrogenation of citronellal to menthol. The MOF was found to be stable under the reaction conditions used, and the results obtained indicate that the performance of this bifunctional solid catalyst is comparable with other state-of-the-art materials for the tandem reaction: Full citronellal conversion was attained over Pd@MIL-101 in 18 h, with 86% selectivity to menthols and a diastereoselectivity of 81% to the desired (-)-menthol, while up to 30 h were necessary for attaining similar values over Ir/H-beta under analogous reaction conditions.
Characterization and Tribological Evaluation of 1-Benzyl-3-Methylimidazolium Bis(trifluoromethylsulfonyl)imide as Neat Lubricant and Oil Additive (2011)

Selected physical and chemical properties and tribological data for a newly-developed, imidazolium-based ionic liquid (IL) are presented. The IL is soluble in the SAE 5W-30 oil up to a certain weight percentage, and is as a promising candidate for use in lubrication applications, either in its neat version or as an oil additive. Characterization of the IL included dynamic viscosity at different temperatures, corrosion effects on cast iron cylinder liners, and thermal stability analysis. The tribological performance was evaluated using a reciprocating ring-on-liner test arrangement. When used in neat version this IL demonstrated friction coefficient comparable to a fully formulated engine oil, and when used as an oil additive it produced less wear.
Sheared polymer network liquid crystal for fast-response variable optical attenuators (2005)

A variable optical attenuator (VOA) at &#955;=1.55 &#956;m using a sheared polymer network liquid crystal (SPNLC) is demonstrated. SPNLC is fabricated by mixing ~15 wt % photo-polymerizable monomer in a LC host. To polymerize the SPNLC cell, a two-step UV curing process was adopted. Before shearing, the cell scatters light strongly, but after shearing the cell becomes highly transparent in the near IR region. Using an E7-based SPNLC, the attenuation of our VOA is rather insensitive to wavelength over the ITU C-band. The rise time and decay time were measured to be 35 &#956;s and 205 &#956;s, respectively, at room temperature. Such a response time is at least one order magnitude faster than the state-of-the-art nematic competitors. Comparing with other polymer-stabilized liquid crystals, the SPNLC exhibits a lower driving voltage and negligible light scattering loss in the IR spectral region. A reflection type, polarization-independent VOA with ~240 &#956;s response time and -32 dB dynamic range was demonstrated at room temperature and 35 V<sub>rms</sub> voltage.
ChemInform Abstract: Syntheses and Aggregate Study of Bisphenol‐Containing Diaza‐18‐Crown‐6 Ligands. (1999)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
59.2: A Novel LED‐Backlight System with Tilted Cylindrical Surfaces on the Light Guide Plate (2013)

Abstract We have developed a new LED‐backlight structure which provides high efficiency with the thin profile. By applying this new optical system, power consumption can be reduced 15.5% compared with conventional thin structure as well as achieving the good luminance uniformity.
Nanoscale Smoothing in Crystallization of Amorphous Indium Tin Oxide Thin Films Induced by Glass Ultrathin Overlayer (2006)

Nanoscale smoothing in the crystallization of amorphous indium tin oxide (ITO) thin films was achieved using an ultrathin silicate glass overlayer (about 3 nm thick). An amorphous ITO thin film with an ultrathin glass overlayer were deposited by pulsed laser ablation using the ITO ceramics and silicate glass targets, respectively. The amorphous ITO film covered by the ultrathin glass layer was crystallized by annealing at 700 °C. When the ultrathin glass layer was removed by wet etching in aqueous H3PO4, an ultrasmooth crystallized ITO film surface appeared. The surface roughness of the crystallized thin film (about 0.3 nm rms) was one order of magnitude smaller than that of the ITO films crystallized with no glass overlayer (3.1 nm rms).
Synthesis of an Exhaustive Library of Naturally Occurring Gal f -Man p and Gal p -Man p Disaccharides. Toward Fingerprinting According to Ring Size by Advanced Mass Spectrometry-Based IM-MS and IRMPD (2021)

Nature offers a huge diversity of glycosidic derivatives. Among numerous structural modulations, the nature of the ring size of hexosides may induce significant differences on both biological and physicochemical properties of the glycoconjugate of interest. On this assumption, we expect that small disaccharides bearing either a furanosyl entity or a pyranosyl residue would give a specific signature, even in the gas phase. On the basis of the scope of mass spectrometry, two analytical techniques to register those signatures were considered, i.e., the ion mobility (IM) and the infrared multiple photon dissociation (IRMPD), in order to build up cross-linked databases. d-Galactose occurs in natural products in both tautomeric forms and presents all possible regioisomers when linked to d-mannose. Consequently, the four reducing Gal<i>f</i>-Man<i>p</i> disaccharides as well as the four Gal<i>p</i>-Man<i>p</i> counterparts were first synthesized according to a highly convergent approach, and IM-MS and IRMPD-MS data were second collected. Both techniques used afforded signatures, specific to the nature of the connectivity between the two glycosyl entities.
Effects of vibration on the proteome expression of anterior cruciate ligament cells (2011)

Recent reports have suggested that vibration has beneficial effects on knee healing response; however, the biomechanism of these beneficial effects still need to be determined on the anterior cruciate ligament (ACL) cell level. In this study, we applied a 20 Hz vibration to ACL cells, which produced a 20% increase (P < 0.001) in cell activity and 17% increase (P < 0.001) in intracellular sulfated glycosaminoglycan levels. In the 20 Hz vibration-stimulated ACL cell group, eight up-regulated (100 ∼ 300%) protein spots were identified compared with the control group by proteomics analysis. Among these proteins, Annexin A2 and Prolyl 4 hydroxylase (PH4B) were shown to have a 71% and 16% higher expression, respectively, in the 20 Hz vibration-stimulated ACL cell group by Western blotting (P < 0.001). These results indicate that vibration produces a positive cellular environment, and Annexin A2 and prolyl 4 hydroxylase are expected to help ligament repair and ACL cell proliferation by controlling cell membrane and extracellular matrix formation.
Cambiarenes: Single‐Step Synthesis and Selective Zwitterion Binding of a Clip‐Shaped Macrocycle with a Redox‐Active Core (2019)

A novel macrocyclic host molecule was synthesized that forms in a single step from commercially available starting materials. The core of the macrocycle backbone possesses two quinone rings and, thus, it is redox-active. Host-guest binding involving the clip-shaped cavity indicates selective binding of pyridine N-oxides based on the electron density of and steric bulk around the anionic oxygen.
Increased endogenous DNA oxidation correlates to increased iron levels in melanocytes relative to keratinocytes. (2015)

The endogenous oxidative state of normal human epidermal melanocytes was investigated and compared to normal human epidermal keratinocytes (NHEKs) in order to gain new insight into melanocyte biology. Previously, we showed that NHEKs contain higher levels of hydrogen peroxide (H2O2) than melanocytes and that it can migrate from NHEKs to melanocytes by passive permeation. Nevertheless, despite lower concentrations of H2O2, we now report higher levels of oxidative DNA in melanocytes as indicated by increased levels of 8-oxo-2'-deoxyguanosine (8-oxo-dG): 4.49 (±0.55 SEM) 8-oxo-dG/10(6) dG compared to 1.49 (±0.11 SEM) 8-oxo-dG/10(6) dG for NHEKs. An antioxidant biomarker, glutathione (GSH), was also lower in melanocytes (3.14 nmoles (±0.15 SEM)/cell) in comparison to NHEKs (5.98 nmoles (±0.33 SEM)/cell). Intriguingly, cellular bioavailable iron as measured in ferritin was found to be nearly fourfold higher in melanocytes than in NHEKs. Further, ferritin levels in melanocytes were also higher than in hepatocarcinoma cells, an iron-rich cell, and it indicates that higher relative iron levels may be characteristic of melanocytes. To account for the increased oxidative DNA and lower GSH and H2O2 levels that we observe, we propose that iron may contribute to higher levels of oxidation by reacting with H2O2 through a Fenton reaction leading to the generation of DNA-reactive hydroxyl radicals. In conclusion, our data support the concept of elevated oxidation and high iron levels as normal parameters of melanocytic activity. We present new evidence that may contribute to our understanding of the melanogenic process and lead to the development of new skin care products.
Chapter 8 THIRD-PARTY REPRODUCTIVE ASSISTANCE AROUND THE MEDITERRANEAN: COMPARING SUNNI EGYPT, CATHOLIC ITALY, AND MULTISECTARIAN LEBANON (2022)

Chapter 8 THIRD-PARTY REPRODUCTIVE ASSISTANCE AROUND THE MEDITERRANEAN: COMPARING SUNNI EGYPT, CATHOLIC ITALY, AND MULTISECTARIAN LEBANON was published in Islam and Assisted Reproductive Technologies on page 223.