gebhart/patrepeval huggingface dataset:
Measuring emission cross-section profile of erbium-dopedfibre withlow input power (1994)

A simple and elegant method is proposed to measure the emission cross-section profile of erbium-doped fibre by using a short piece of erbium-doped fibre with input pump or signal power less than 1 mW. Relative error is within 1% over the whole spectrum. The theoretical and experimental results are discussed.
Enhanced pelvic responses to stressors in female CRF-overexpressing mice (2006)

Acute stress affects gut functions through the activation of corticotropin-releasing factor (CRF) receptors. The impact of acute stress on pelvic viscera in the context of chronic stress is not well characterized. We investigated the colonic, urinary, and locomotor responses monitored as fecal pellet output (FPO), urine voiding, and ambulatory activity, respectively, in female and male CRF-overexpressing (CRF-OE) mice, a chronic stress model, and their wild-type littermates (WTL). Female CRF-OE mice, compared with WTL, had enhanced FPO to 2-min handling (150%) and 60-min novel environment (155%) but displayed a similar response to a 60-min partial restraint stress. Female CRF-OE mice, compared with WTL, also had a significantly increased number of urine spots (7.3 +/- 1.4 vs. 1.3 +/- 0.8 spots/h) and lower locomotor activity (246.8 +/- 47.8 vs. 388.2 +/- 31.9 entries/h) to a novel environment. Male CRF-OE mice and WTL both responded to a novel environment but failed to show differences between them in colonic and locomotor responses. Male WTL, compared with female WTL, had higher FPO (113%). In female CRF-OE mice, the CRF(1)/CRF(2) receptor antagonist astressin B and the selective CRF(2) receptor agonist mouse urocortin 2 (injected peripherally) prevented the enhanced defecation without affecting urine or locomotor responses to novel environment. RT-PCR showed that CRF(1) and CRF(2) receptors are expressed in the mouse colonic tissues. The data show that chronic stress, due to continuous central CRF overdrive, renders female CRF-OE mice to have enhanced pelvic and altered behavioral responses to superimposed mild stressors and that CRF(1)-initiated colonic response is counteracted by selective activation of CRF(2) receptor.
Research in Macromolecular Science: Challenges and Opportunities for the Next Decade (2008)

ADVERTISEMENT RETURN TO ISSUEPerspectiveNEXTResearch in Macromolecular Science: Challenges and Opportunities for the Next DecadeC. K. Ober†, S. Z. D. Cheng‡, P. T. Hammond§, M. Muthukumar∥, E. Reichmanis⊥, K. L. Wooley#, and T. P. Lodge*∇View Author Information Department of Materials Science and Engineering, Cornell University, Ithaca, New York 14853; Department of Polymer Science, University of Akron, Akron, Ohio 44325; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139; Department of Polymer Science and Engineering, University of Massachusetts, Amherst, Massachusetts 01003; School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332; Department of Chemistry and Department of Radiology, Washington University, St. Louis, Missouri 63130; and Department of Chemistry and Department of Chemical Engineering & Materials Science, University of Minnesota, Minneapolis, Minnesota 55455* To whom correspondence should be addressed. E-mail: [email protected]†Cornell University.‡University of Akron.§Massachusetts Institute of Technology.∥University of Massachusetts.⊥Georgia Institute of Technology.#Washington University.∇University of Minnesota.Cite this: Macromolecules 2009, 42, 2, 465–471Publication Date (Web):December 10, 2008Publication History Received3 November 2008Published online10 December 2008Published inissue 27 January 2009https://pubs.acs.org/doi/10.1021/ma802463zhttps://doi.org/10.1021/ma802463zreview-articleACS PublicationsCopyright © 2008 American Chemical SocietyRequest reuse permissionsArticle Views9594Altmetric-Citations139LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Chemical structure,Materials,Polymer science,Polymers,Supramolecular structures and assemblies Get e-Alerts
Genetic and Neuroimaging Approaches to Understanding Post-Traumatic Stress Disorder (2020)

Post-traumatic stress disorder (PTSD) is a highly disabling condition, increasingly recognized as both a disorder of mental health and social burden, but also as an anxiety disorder characterized by fear, stress, and negative alterations in mood. PTSD is associated with structural, metabolic, and molecular changes in several brain regions and the neural circuitry. Brain areas implicated in the traumatic stress response include the amygdala, hippocampus, and prefrontal cortex, which play an essential role in memory function. Abnormalities in these brain areas are hypothesized to underlie symptoms of PTSD and other stress-related psychiatric disorders. Conventional methods of studying PTSD have proven to be insufficient for diagnosis, measurement of treatment efficacy, and monitoring disease progression, and currently, there is no diagnostic biomarker available for PTSD. A deep understanding of cutting-edge neuroimaging genetic approaches is necessary for the development of novel therapeutics and biomarkers to better diagnose and treat the disorder. A current goal is to understand the gene pathways that are associated with PTSD, and how those genes act on the fear/stress circuitry to mediate risk vs. resilience for PTSD. This review article explains the rationale and practical utility of neuroimaging genetics in PTSD and how the resulting information can aid the diagnosis and clinical management of patients with PTSD.
Applied network slicing scenarios in 5G (2021)

Private 5G networks - the new business offerings that network slicing enables – deliver functionality that extends well beyond that of the current offerings that are typically based on existing public network services. For example, network slicing makes it possible to create a private 5G network with specific service characteristics as well as varying degrees of security/isolation, exposure, self-management, and so on.
Structure of conkunitzin-S1, a neurotoxin and Kunitz-fold disulfide variant from cone snail (2006)

Cone snails (Conus) are predatory marine mollusks that immobilize prey with venom containing 50-200 neurotoxic polypeptides. Most of these polypeptides are small disulfide-rich conotoxins that can be classified into families according to their respective ion-channel targets and patterns of cysteine-cysteine disulfides. Conkunitzin-S1, a potassium-channel pore-blocking toxin isolated from C. striatus venom, is a member of a newly defined conotoxin family with sequence homology to Kunitz-fold proteins such as alpha-dendrotoxin and bovine pancreatic trypsin inhibitor (BPTI). While conkunitzin-S1 and alpha-dendrotoxin are 42% identical in amino-acid sequence, conkunitzin-S1 has only four of the six cysteines normally found in Kunitz proteins. Here, the crystal structure of conkunitzin-S1 is reported. Conkunitzin-S1 adopts the canonical 3(10)-beta-beta-alpha Kunitz fold complete with additional distinguishing structural features including two completely buried water molecules. The crystal structure, although completely consistent with previously reported NMR distance restraints, provides a greater degree of precision for atomic coordinates, especially for S atoms and buried solvent molecules. The region normally cross-linked by cysteines II and IV in other Kunitz proteins retains a network of hydrogen bonds and van der Waals interactions comparable to those found in alpha-dendrotoxin and BPTI. In conkunitzin-S1, glycine occupies the sequence position normally reserved for cysteine II and the special steric properties of glycine allow additional van der Waals contacts with the glutamine residue substituting for cysteine IV. Evolution has thus defrayed the cost of losing a disulfide bond by augmenting and optimizing weaker yet nonetheless effective non-covalent interactions.
Novel Antibodies for the Simple and Efficient Enrichment of Native O-GlcNAc Modified Peptides (2021)

Antibodies against posttranslational modifications (PTMs) such as lysine acetylation, ubiquitin remnants, or phosphotyrosine have resulted in significant advances in our understanding of the fundamental roles of these PTMs in biology. However, the roles of a number of PTMs remain largely unexplored due to the lack of robust enrichment reagents. The addition of N-acetylglucosamine to serine and threonine residues (O-GlcNAc) by the O-GlcNAc transferase (OGT) is a PTM implicated in numerous biological processes and disease states but with limited techniques for its study. Here, we evaluate a new mixture of anti-O-GlcNAc monoclonal antibodies for the immunoprecipitation of native O-GlcNAcylated peptides from cells and tissues. The anti-O-GlcNAc antibodies display good sensitivity and high specificity toward O-GlcNAc-modified peptides and do not recognize O-GalNAc or GlcNAc in extended glycans. Applying this antibody-based enrichment strategy to synaptosomes from mouse brain tissue samples, we identified over 1300 unique O-GlcNAc-modified peptides and over 1000 sites using just a fraction of sample preparation and instrument time required in other landmark investigations of O-GlcNAcylation. Our rapid and robust method greatly simplifies the analysis of O-GlcNAc signaling and will help to elucidate the role of this challenging PTM in health and disease.
Compensation of nonlinear effects in optical communication systems through phase-conjugation (2009)

This work demonstrates that the use of the mid-nonlinearity temporal inversion (MNTI) technique could allow the realization of efficient transmission systems at ultra-high bit rates, leading to a significant impact on the design, assessment, and implementation of future transmission links. In particular, this technique could open the way to the low cost upgrade of standard low bit-rate systems to 10Gb/s or multi-level, phase-modulated systems.
Innentitelbild: A Phosphine‐Mediated Conversion of Azides into Diazo Compounds (Angew. Chem. 13/2009) (2009)

Abstract Im Jahr 1919 berichteten H. Staudinger und J. Meyer über die reduktive Fragmentierung eines Azids zu einem Amin. Auf S. 2395 ff. stellen nun E. L. Myers und R. T. Raines ein ergänzendes Verfahren vor: die Spaltung der anderen N‐N‐Bindung eines Azids unter Bildung einer Diazoverbindung. Weil in beiden Umsetzungen ein Phosphor(III)‐Reagens zur Anwendung kommt, wurde als Hintergrund das Bild The Alchymist, in Search of the Philosopher's Stone, Discovers Phosphorus aus dem Jahr 1771 gewählt, in dem Joseph Wright die Entdeckung des Phosphors durch Hennig Brandt im Jahr 1669 darstellt. (Gestaltung: H. Adam Steinberg) magnified image
Antiviral studies of feline infectious peritonitis virus in vitro (1994)

Sixteen compounds were tested for their ability to inhibit the replication in vitro of feline infectious peritonitis virus (FIPV), a coronavirus that causes a lethal, immunologically mediated illness in domestic and exotic cats. Six of the compounds, when incubated with cells and titrations of the virus, were found to reduce the virus titres by 0.401 to 0.833 log10 (P < 0.05), using the cytopathic effect as endpoint. Further inhibition studies were performed to determine the 50 per cent effective dose (ED50) levels for these six compounds. Selectivity indices (50 per cent cytotoxic dose [CD50]/ED50) provided the following order of antiviral activity: pyrazofuin > 6-azauridine > 3-deazaguanosine > hygromycin B > fusidic acid > dipyridamole. Compounds which had no statistically significant effect on FIPV in the same assay were caffeic acid, carbodine, 3-deazauridine, 5-fluoroorotic acid, 5-fluorouracil, D(+)glucosamine, indomethacin, D-penicillamine, rhodamine and taurine.
Whole Body Protein Turnover in Overweight/Obese Adults in Response to Whole Grain and Refined Grain Diets – A Randomized Control Trial (2016)

Data from several intervention studies suggest that whole grains (WG) lead to increased fat loss and enhanced metabolic function compared to control diets. Abnormalities in energy balance and protein metabolism, including protein flux, protein synthesis and protein breakdown, are part of the obesity phenotype and may be amenable to change by altering nutrient intake using a WG enriched diet. We hypothesized that a whole grain enriched diet would alter protein metabolism and total energy expenditure (TEE), and thus contribute to increased muscle mass and a decrease in fat mass. We performed a double‐blind crossover diet intervention study to examine the effects of a WG‐based diet on protein kinetics, TEE and body composition, compared to a calorie matched control diet (refined grain (RG)). Complete diets were provided to subjects, based on nutritional guidelines for fat, protein (1.05 g/kg/day) and carbohydrate, with either WG (50g/1000 kcal) or equivalent RG. Fourteen overweight/obese (BMI 32.9 ± 4.5 kg/m 2 ) adults (age 39 ± 7 years) participated in the trial. Each diet was provided for 8 weeks, with a 10‐week washout between diet arms. TEE was assessed by doubly labeled water ( 2 H 2 18 O), whole body protein turnover was assessed using a stable isotope approach (oral dose of 15 N‐Glycine), and body composition was determined from DEXA measures. After 8 weeks of WG and RG diet, changes in body weight and fat free mass were similar for both diets. Likewise, TEE did not differ between the diets (~2147 ± 291 kcal/day). Protein synthesis was 3.7 ± 0.5 g protein/kg/day for both diets (P&gt;0.05), while protein breakdown was 4.7 ± 0.5 and 4.9 ± 0.4 g protein/kg/day for WG and RG diets, respectively. For protein balance the values were −1.03 ± 0.05 and −1.1 ± 0.1 g, protein/kg/day for WG and RG diets (P&gt;0.05), respectively. Rates of protein synthesis, breakdown and nitrogen balance were higher after the WG diet when expressed relative to FFM, but protein turnover was similar for effects between diets. We conclude that 8 weeks on a WG diet does not produce significantly different changes in body composition, TEE, or whole body protein metabolism, when compared to a calorie matched control diet. Support or Funding Information Investigator‐initiated grant, Nestlé; NIH UL1RR024989 and T32 DK007319.
111 RecA mediated homology recognition offers lessons for sequence dependent protein recognition, protein folding, and artificial self-assembly (2015)

In vivo, RecA mediated homology recognition must stringently reject non-homologous pairings, but it must also allow strand exchange to progress through non-homologous regions within homologous pair...
From 5-Azapyrimidine Chemistry to Thiadiazoles (2022)

The review is devoted to 5-aza- and 6-azapyrimidine nucleosides with emphasis on antileukemic agents 5‑azacytidine and 2′-deoxy-5-azacytidine (decitabine), mechanism of their action as inhibitors of DNA methylations in tumour suppressor genes and current trends in the search for their stabilized analogues. A significant contribution to 5-azacytosine chemistry is synthesis of 5-aza­cytosine acyclic nucleosides phosphonates and their prodrugs, identified as potent and selective anti-DNA viral agents. Application of 5-amino-1,2,4-thiadiazol-3-(2H)-one moiety to acyclic nucleoside analogues was inspired by hypothesis that this heterocyclic base could work as a cytosine mimic and, moreover, the reactive N-S bond could be able to attack strong nucleophiles such as the cysteine thiol group of enzymes (similarly as 5‑azacytosine). A series of prepared 1,2,4-thiadiazole derivatives provided several inhibitors of Cathepsin K and glycogen synthase kinase 3β effective in the low micromolar range.
Doppler Mitigation in OFDM-Based Aeronautical Communications (2014)

Doppler spectrum in an aeronautical channel is modeled with dual-Doppler shift instead of classical Jakes model. Orthogonal frequency division multiplexing (OFDM)-based systems are sensitive to Doppler shifts/spread, since the time variation of the channel causes inter-carrier interference (ICI). ICI analysis is provided here for OFDM-based systems in the aeronautical channel. The effect of ICI on the received signal is presented and its power is derived. As compared with terrestrial channels, where ICI is generally overcome by increasing the subcarrier spacing and bounding the normalized Doppler frequency (NDF), we propose to mitigate the effect of Doppler shifts in aeronautical channels, as follows. First, we use parametric spectrum estimation methods to extract the Doppler shifts by exploiting the predictable number of paths. Then, a beamforming-based method is introduced to resolve the incoming rays by separating them individually. Finally, paths are Doppler-compensated and combined using diversity combining techniques. Computer simulations are performed to provide numerical results. It is shown that a mean square error (MSE) performance ofis achieved with the parametric estimation methods, and bit error rate (BER) performance approaching the no-Doppler scenario is obtained with the beamforming-based mitigation method.
Supplementary Figure 5 from Skin-Test Infiltrating Lymphocytes Early Predict Clinical Outcome of Dendritic Cell–Based Vaccination in Metastatic Melanoma (2023)

&lt;p&gt;PDF file - 73K, Patient selection based on SKIL criteria: SKIL criteria are applied to select patients with immune responses meeting increasingly stringent criteria&lt;/p&gt;
Enhanced photoelectrochemical performance of composite photovoltaic cells of Li+@C60–sulphonated porphyrin supramolecular nanoclusters (2013)

A photoelectrochemical solar cell composed of supramolecular nanoclusters of lithium encapsulated fullerene and zinc sulphonated meso-tetraphenylporphyrin exhibits significant enhancement in the photoelectrochemical performance as compared with the reference system containing only a single component.
Conformational Analysis of Chiral Supramolecular Aggregates: Modeling the Subtle Difference between Hydrogen and Deuterium (2013)

A detailed analysis of the conformational states of self-assembled, stereoselectively deuterated benzene-1,3,5-tricarboxamides ((S,S,S)-D-BTAs) reveals four different conformers for the supramolecular polymers. The relative amount of the conformers depends on the solvent structure and the temperature. With the help of a model, the thermodynamic parameters that characterize the different conformational states were quantified as well as the amount of the species that occur at different stages of the polymerization process. The results show that small changes in the stability between different types of conformers formed by (S,S,S)-D-BTAs—in the order of a few J mol(–1)—arise from the combination of interactions between the solvent/supramolecular aggregate, temperature, and solvent structure. While the introduction of a deuterium label allows to sensitively probe the solvophobic effects in the supramolecular aggregation, a rationalization of the observed effects on a molecular level is not yet straightforward but is proposed to result from subtle effects in the vibrational enthalpy and entropy terms of the isotope effect.
Muscle injury promotes heterotopic ossification by stimulating local bone morphogenetic protein-7 production (2019)

Our results provide a candidate mechanistic rationale for the use of antiinflammatory drugs in the prevention of HO.
Factors affecting click-through behavior in aggregated search interfaces (2010)

An aggregated search interface is designed to integrate search results from different sources (web, image, video, blog, etc) into a single result page. This paper presents two user studies investigating factors affecting users click-through behavior on aggregated search interfaces. We tested two aggregated search interfaces: one where results from the different sources are blended into a single list (called blended), and another, where results from each source are presented in a separate panel (called non-blended). A total of 1,296 search sessions performed by 48 participants were analysed in our study. Our results suggest that 1) the position of search results is significant only in the blended and not in the non-blended design; 2) participants' click-through behavior on videos is different from other sources; and finally 3) capturing a task's orientation towards particular sources is an important factor for further investigation and research.
Cross-flow membrane filtration of cell suspensions (1989)

Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1989.
Irritable bowel syndrome is strongly associated with generalized anxiety disorder: a community study (2009)

Irritable Bowel Syndrome and GAD comorbidity was common and added to impairment in the community. The strong association between psychiatric morbidity and IBS observed in referral centres was not a consequence of increased help-seeking behaviour.
Supersonic bare metal cluster beams (1991)

This paper discusses the production and some applications of bare metal clusters through beam techniques. (LSP)
Immunoglobulin variable region hypermutation in hybrids derived from a pre-B- and a myeloma cell line. (1995)

Somatic mutation of the variable (V) regions of immunoglobulin genes occurs in vivo at rates that have been estimated to be between 10(-3) and 10(-4) per bp per generation. To study this process in vitro, the 18.81 pre-B-cell line and hybrids derived by fusing 18.81 to the NSO myeloma fusion partner were transfected with a mu heavy-chain construct containing a nonsense mutation in the V region (Vn) or the constant region (Cn). Mutation was quantitated by reversion analysis using the ELISA spot assay to detect single cells secreting IgM. Fluctuation analysis revealed that V-region mutations spontaneously occurred in 18.81 cells at an average rate of 5.8 x 10(-6) per bp per cell generation and in selected 18.81-NSO hybrids at greatly increased rates of 1.6 x 10(-3) to 5.8 x 10(-4) per bp per generation. The Vn construct also reverted frequently in transgenic mice, indicating that it contained sufficient information to mutate at high rates both in vivo and in vitro. Sequence analysis of reverted genes revealed that reversion was due to point mutations. Since the rates and nature of the mutations that are occurring in these transfected genes are similar to those reported in vivo, it should be possible to use this system to identify the cis-acting sequences and trans-acting factors that are responsible for V-region somatic hypermutation.