gebhart/patrepeval huggingface dataset:
Cellular Arachidonate-releasing Function of Novel Classes of Secretory Phospholipase A2s (Groups III and XII) (2003)

Here we report cellular arachidonate (AA) release and prostaglandin (PG) production by novel classes of secretory phospholipase A(2)s (sPLA(2)s), groups III and XII. Human group III sPLA(2) promoted spontaneous AA release, which was augmented by interleukin-1, in HEK293 transfectants. The central sPLA(2) domain alone was sufficient for its in vitro enzymatic activity and for cellular AA release at the plasma membrane, whereas either the unique N- or C-terminal domain was required for heparanoid-dependent action on cells to augment AA release, cyclooxygenase-2 induction, and PG production. Group III sPLA(2) was constitutively expressed in two human cell lines, in which other sPLA(2)s exhibited different stimulus inducibility. Human group XII sPLA(2) had a weak enzymatic activity in vitro and minimally affects cellular AA release and PG production. Cells transfected with group XII sPLA(2) exhibited abnormal morphology, suggesting a unique functional aspect of this enzyme. Based on the present results as well as our current analyses on the group I/II/V/X sPLA(2)s, general properties of cellular actions of a full set of mammalian sPLA(2)s in regulating AA metabolism are discussed.
Gas phase dimerization of neuropeptide head activator analogs useful for the noncovalent constraint of peptides (2006)

We have used electrospray mass spectrometry to examine the dimerization of EFLIVKS, a reversed sequence analog of part of the neuropeptide head activator, and other similar analogs. Observation of the EFLIVKS gas phase dimer is concentration-dependent, with a half-saturation concentration for relative dimer formation of 7.8 microM, similar to that of SKVILFE of 12 microM. The lowest energy conformers from quenched molecular dynamics simulations suggest EFLIVKS may dimerize in the gas phase by formation of multiple ion pairs across the dimer interface. Alanine-scan mutants also dimerize in the gas phase, with replacement of the interior residues FLIVK diminishing dimerization. The concentration-dependence of the EFLIVKS circular dichroism spectrum at pH 7.5 suggests the existence of different conformation states at different concentrations, but does not provide evidence supporting the saturable dimer formation in solution. Different analogs of EFLIVKS, when fused to each end of a 18mer unfolded peptide, induce solution structures with T(m)s of 42-50 degrees C. These peptides and analogs may thus be useful for the noncovalent constraint of peptides and peptide library members used in cellular screens.
Circulating platelet aggregates in sickle cell disease patients with and without vaso-occlusion. (1979)

In vivo circulating platelet aggregates (CPA) were evaluated in 18 patients aged 6 to 17 years with sickle cell disease and in 11 age and sex matched normal subjects. Twelve patients with sickle cell disease were in steady state and 6 had vaso-occlusive crises. CPA in patients in steady state were similar to those in normal subjects (mean 6 +/- 1% compared to 5 +/- 2%, respectively), whereas patients with vaso-occlusive crisis in acute state had significantly higher CPA (mean 39 +/- 8%) than patients in steady state or normal control individuals (both p less than 0.001). CPA decreased in patients with vaso-occlusive crisis (mean 11 +/- 4%) on the tenth day, in association with clinical improvement. This study suggests that in vivo platelet aggregate formation activity, although normal in sickle cell disease patients in steady state, is significantly increased in patients with vaso-occlusive crises.
Dynamic mechanical behavior of continuous multilayer composites (1987)

Abstract The dispersion processes in multilayer laminate composites of styrene acrylonitrile (SAN) and polycarbonate (PC) were studied utilizing the torsion pendulum. A third damping peak with a log decrement intensity of approximately one was observed at a temperature intermediate to the damping peaks corresponding to the T g 's of the two constituent phases. Variations of numerous material and experimental parameters such as composition ratio, orientation, thermal history, thermal cycling, number of layers, and layer thickness, as well as overall changes in the composition of the phases had no effect on the observance of a third peak. Only the disruption of the continuous layer structure effectively eliminated this novel transition. The origin of this transition was explained by assuming appropriate temperature dependencies for the controlling viscoelastic parameters in such a continuous layer composite.
Ibrutinib Enhances the Anti-Tumor Immune Response Induced By Intratumoral Injection of a TLR9 Ligand (2014)

Abstract We have designed a novel approach for treating tumors utilizing a combination of active immunotherapy and targeted kinase inhibition. We injected tumors with unmethylated CG-enriched oligodeoxynucleotides (CpG), an agonist for the toll like receptor 9 (TLR9). This in situ CpG injection resulted in local tumor eradication by NK cells and macrophages but on its own was not able to induce a systemic anti-tumor immune response. Ibrutinib is an irreversible inhibitor of Bruton's tyrosine kinase (Btk), a key enzyme in the signaling pathway downstream of B Cell receptor (BCR) is now known to also inhibit ITK, a key enzyme in the survival of Th2 T cells. By doing so, it can shift the balance to the more effective Th1 immune response. In our murine model, the combination of in situ injection of CpG and systemic treatment with Ibrutinib resulted in eradication of tumor cells not only in the local site but also at distant sites. This anti-tumor immune response required both CD4 and CD8 T cells as the effect was lost in nude, scid and T cell depleted mice. Additionally, transferred T cells from animals previously treated with CpG and Ibrutinib protected na•ve mice from tumor challenge. This immune-enhancing effect of Ibrutinib is unexpected. It reflects on the mechanism of action of the drug against B cell malignancies and it provides a novel way of enhancing anti-tumor immune responses against other types of cancer. Figure 1 Figure 1. A. six to eight weeks BALB/C mice were inoculated with 1x106 H11 cells s.c. into the right and left sides of their abdomen, tumor growth was monitored with a digital caliper. Therapy was started when tumors reached a size of 0.7-1cm in the largest diameter, CpG (100µg/injection) was given intratumorally to the left tumor every day in days 1-5, Ibrutinib (6mg/kg) was given daily, intraperitonealy (IP) on days 1-8. B. Growth curve of the right (non-treated) tumor C. Mice survival D. Mice at day 4 after treatment. Disclosures No relevant conflicts of interest to declare.
Workload optimization using SharedDB (2013)

This demonstration presents SharedDB, an implementation of a relational database system capable of executing all SQL operators by sharing computation and resources across all running queries. SharedDB sidesteps the traditional query-at-a-time approach and executes queries in batches. Unlike proposed multi-query optimization ideas, in SharedDB queries do not have to contain common subexpressions in order to be part of the same batch, which allows for a higher degree of sharing. By sharing as much as possible, SharedDB avoids repeating parts of computation that is common across all running queries. The goal of this demonstration is to show the ability of shared query execution to a) answer complex and diverse workloads, and b) reduce the interaction among concurrently executed queries that is observed in traditional systems and leads to performance deterioration and instabilities.
Coxa Vara With Proximal Femoral Growth Arrest in Patients Who Had Neonatal Extracorporeal Membrane Oxygenation (2003)

This is a retrospective review of four patients in whom a pattern of coxa vara with proximal femoral growth arrest and metaphyseal irregularities developed. These patients were all treated with neonatal extracorporeal membrane oxygenation and presented with a progressive gait disturbance and pain, leg-length discrepancy, and limited abduction. Imaging revealed coxa vara with proximal femoral growth arrest. Two patients (three hips) underwent proximal femoral valgus osteotomy, one patient underwent fixation of a femoral neck fracture with subsequent greater trochanter transfer, and one patient is being observed. This case series suggests an association between neonatal extracorporeal membrane oxygenation and this unusual pattern of coxa vara with proximal femoral growth arrest.
Ferret model to mimic the sequential exposure of humans to historical H3N2 influenza viruses (2022)

Mutations accumulate in influenza A virus proteins, especially in the main epitopes on the virus surface glycoprotein hemagglutinin (HA). For influenza A(H3N2) viruses, in particular, the antigenicity of their HA has altered since their emergence in 1968, requiring changes of vaccine strains every few years. Most adults have been exposed to several antigenically divergent H3N2 viruses through infection and/or vaccination, and those exposures affect the immune responses of those individuals. However, animal models reflecting this 'immune history' in humans are lacking and naïve animals are generally used for vaccination and virus challenge studies. Here, we describe a ferret model to mimic the serial exposure of humans to antigenically different historical H3HA proteins. In this model, ferrets were sequentially immunized with adjuvanted recombinant H3HA proteins from two or three different H3HA antigenic clusters in chronological order, and serum neutralizing antibody titers were examined against the homologous virus and viruses from different antigenic clusters. For ferrets immunized with a single HA antigen, serum neutralizing antibody titers were elevated specifically against the homologous virus. However, after immunization with the second or third antigenically distinct HA antigen in chronological order, the ferrets showed an increase in more broadly cross-reactive neutralizing titers against the antigenically distinct viruses and against the homologous virus. Sequentially immunized animals challenged with an antigenically advanced H3N2 virus showed attenuated virus growth and less body temperature increase compared with naïve animals. These results suggest that sequential exposure to antigenically different HAs elicits broader neutralizing activity in sera and enhances immune responses against more antigenically distinct viruses Our findings may partly explain why adults who have been exposed to antigenically divergent HAs are less likely to be infected with influenza virus and have severe symptoms than children.
Apoptosis in testis germ cells: developmental changes in gonadotropin dependence and localization to selective tubule stages. (1995)

Recent studies have demonstrated apoptotic DNA fragmentation in the testis of immature rats deprived of gonadotropins. However, the exact cell type undergoing apoptosis during testis development and the age differences of gonadotropin dependence of testis cell apoptosis are unclear. The present study used gel fractionation and in situ methods to quantitate developmental changes of testis cell DNA fragmentation and to localize the specific cell type affected in developing rats with and without treatment with a GnRH antagonist. Apoptotic DNA fragmentation in whole testis was measured in rats between 8-70 days of age. A gradual increase (1.8- to 2.0-fold) in testis apoptotic DNA fragmentation was seen in rats between 16-28 days of age, compared with 8-day-old animals, followed by a decrease in adult animals. To study gonadotropin dependence of testicular apoptosis, serum FSH and, to a lesser extent, LH were suppressed by treatment with a long-acting GnRH antagonist (azaline-B, 250 micrograms/kg body wt, two injections at 2-day intervals). Pretreatment with the GnRH antagonist increased apoptotic DNA fragmentation in rats between 16-32 days of age but not in younger and adult animals demonstrating an age-related change in gonadotropin dependence. To identify the exact testis cell type undergoing apoptosis, in situ analysis of DNA fragmentation was performed. In rats at 16-24 days of age, spermatocytes in selected tubules were found to have increased DNA fragmentation. In contrast, neither Leydig cells nor Sertoli cells were affected. In 32-day-old and adult animals, increased DNA fragmentation was seen in early primary spermatocytes of some tubules. Treatment with GnRH antagonist increased the number of cells with DNA fragmentation as well as percentage of tubules affected. In animals between 16-32 days of age, meiotic spermatocytes were labeled, whereas early spermatids were also labeled in 24- and 32-day-old animals. In adult animals, the level of apoptotic DNA fragmentation was not affected by GnRH antagonist treatment. However, DNA isolated from specific stages of the seminiferous tubules of adult animals showed stage-specific changes of apoptotic DNA fragmentation with 2-fold higher levels found in stages I and XII-XIV compared with stage VIII. In situ analysis of adult testis demonstrated that spermatocytes were the major cell type affected. In conclusion, the present study demonstrated that at least three factors determine the onset of apoptosis of the male germ cells: 1) the developmental stage of the animal; 2) serum levels of gonadotropins, especially FSH; and 3) specific stage of the seminiferous epithelial cycle. The present approach provides the basis for future analysis of the role of gonadotropins and other factors in the regulation of testis cell degeneration in normal and pathological states.
High‐risk human papilloma virus infection decreases the frequency of dendritic Langerhans' cells in the human female genital tract (2005)

Dendritic cells (DC) are often arranged in planar layers in tissues with high antigenic exposure, such as skin and mucosae. Providing an en face view, this arrangement optimizes in situ analysis regarding morphology (even of individual dendrites), topographic distribution (regular/clustered) and quantification. The few reports on human genital DC usually utilize single markers and conventional sections, restricting immunolabelling only to cell parts sectioned by the cut. To better assess DC in situ, we labelled epithelial sheets, prepared from fresh cervix biopsies, with antibodies to major histocompatibility complex (MHC)-CII, CD1a and Langerin, revealing (with each of these markers) a dense DC network in a planar-like, regular distribution. Using the hybrid capture system to detect the high-risk mucotropic human papilloma virus (HPV) group, 16 positive and five negative women were studied and the results were compared between these groups. DC frequency per area was substantially reduced (to approximately 50% for the three markers) in samples from all HPV-infected patients compared with samples from controls. Unlike HPV(-) samples, Langerin(+) DC in HPV(+) cervix exhibited a highly accentuated dendritic appearance. We believe this to be the first study using these three DC-restricted markers (Langerin, CD1a and MHC-CII) in cervical epithelial sheets from high-risk HPV(+) donors and also the first study to demonstrate the morphological and quantitative changes triggered by high-risk HPV infection. Cervical DC reduction in early, premalignant high-risk HPV infection might represent viral subversion strategies interfering with efficient antigen handling by the immune system's peripheral sentinels, the DC, perhaps hampering appropriate recruitment and subsequent development of effector (cytotoxic) T cells.
Potential for Ablation Therapy in Patients with Brugada Syndrome (2005)

L-F. Hsu, B. Cauchemez, P. Jaïs, P. Sanders, M. Hocini, P. Maury, J. Angel Cabrera, C. Scavée, Y. Takahashi, M. Rotter, J-L. Pasquie, J. Victor, S. Garrigue, J. Clémenty, M. Haïssaguerre
Improving manual skills in persons with disabilities (PWD) through a multimodal assistance system (2013)

The Multimodal Guidance System (MGS) is an interface that offers haptic and sound feedback while performing manual tasks. Several studies demonstrated that the haptic guidance systems can help people in recovering cognitive function at different levels of complexity and impairment. The applications supported by our device could also have an important role in supporting physical therapist and cognitive psychologist in helping patients to recover motor and visuo-spatial abilities.
Cytotoxic T lymphocytes form an antigen-independent ring junction (2004)

Immunological synapses are organized cell-cell junctions between T lymphocytes and APCs composed of an adhesion ring, the peripheral supramolecular activation cluster (pSMAC), and a central T cell receptor cluster, the central supramolecular activation cluster (cSMAC). In CD8 + cytotoxic T lymphocytes, the immunological synapse is thought to facilitate specific killing by confining cytotoxic agents to the synaptic cleft. We have investigated the interaction of human CTLs and helper T cells with supported planar bilayers containing ICAM-1. This artificial substrate provides identical ligands to CD4 + and CD8 + T cells, allowing a quantitative comparison. We found that cytotoxic T lymphocytes form a ring junction similar to a pSMAC in response to high surface densities of ICAM-1 in the planar bilayer. MICA, a ligand for NKG2D, facilitated the ring junction formation at lower surface densities of ICAM-1. ICAM-1 and MICA are upregulated in tissues by inflammation-and stressassociated signaling, respectively. Activated CD8 + T cells formed fivefold more ring junctions than did activated CD4 + T cells. The ring junction contained lymphocyte function associated antigen-1 and talin, but did not trigger polarization and granule translocation to the interface. This result has specific implications for the mechanism of effective CTL hunting for antigen in tissues. Abnormalities in this process may alter CTL reactivity.
Casing Burst Stresses in Particulate-Filled Annuli: Where's the Cement? (2011)

Abstract For burst design, engineers routinely assume the casing annular space is filled by a fluid equivalent. This assumption ignores mechanical resistance provided by solid cement. Some studies addressed this shortcoming by modeling the cement sheath as a solid with elastic failure criteria. Prior work used cement elastic modulus and Poisson ratio to classify cement as ‘ductile’ (soft) or ‘brittle’ (hard). In the current study, numerical results from finite element analysis (FEA) indicate that casing burst resistance is increased by the presence of the cement sheath. This study focuses solely on improvement offered by the cement sheath to casing burst resistance and ignores consequences of cement failure on overall well integrity. Comparisons are provided for casing burst resistance assuming various backup profiles. These include fluid hydrostatics, solid cement matrix (both elastic and plastic response) and cement as ‘loose’ particles. The fluid hydrostatics include: a) mud weight in hole; b) cement slurry density; c) mixed-water density; d) normal pressure (salt-water column); and e) actual pore pressure. Calculations show that these fluid profiles are conservative when used as burst-resistance backup. Original cement slurry density is least conservative. Since well designers are familiar with fluid profile backup assumptions in casing burst design, recommendations are provided to approximate cement behavior as particles with a fluid profile. This allows ease of calculation and is consistent with current practice. Guidelines are provided to explicitly calculate the enhanced casing burst resistance due to the particulate cement.
Stimulated Brillouin scattering in a fiber waveguide with linear amplification (1987)

A theoretical investigation is made of the influence of linear amplification as a result of stimulated Brillouin scattering in a multimode fiber waveguide. Calculations are made of the threshold of such scattering and of the efficiency of conversion of a pump wave into a Stokes wave under steady-state and transient conditions. The theoretical results are shown to agree well with experimental results obtained under small-gain conditions.
The safety of weekly low dose oral methotrexate in an Oriental population with rheumatoid arthritis. (1997)

A retrospective review of 50 oriental patients with rheumatoid arthritis treated with weekly oral methotrexate showed adverse events in 15 (30%) patients with 19 occurrences (38%) of leucopaenia (4%), pancytopaenia (2%), gastrointestinal symptoms (18%), hepatic transaminase elevation (6%), rash (2%) and infections (6%). The median duration of treatment with methotrexate was 11 months (range 1 to 105 months). Pancytopaenia occurred in 1 patient with renal failure. All adverse events resolved with cessation of therapy and on several occasions, despite continued therapy. Methotrexate was discontinued permanently in 2 and temporarily in 7 patients as a result of adverse events. No recurrence of adverse events was noted on restarting methotrexate therapy in patients with non life-threatening adverse events. No increase in adverse events was noted in 14 patients treated with a combination of methotrexate and anti-malarial therapy. We conclude that methotrexate was well tolerated by the Oriental patients with rheumatoid arthritis in our study and could be safely restarted in those patients with non life-threatening adverse events.
Nested Square Roots of 2 (2003)

Click to increase image sizeClick to decrease image size This article is part of the following collections: Mathematics of Pi
Efficient Transfer of a VA-SWNT Film by a Flip-Over Technique (2006)

A transfer of a VA-SWNT film onto a conductive surface has been achieved using a novel "flip-over" technique. The top surface of a VA-SWNT film was covered by entangled bundles in an as-grown sample. When a VA-SWNT film was flipped over, an optically flat surface consisting of the tips of very well aligned, clean bundles from the bottom of the film are exposed while the top of the film is well contacted to the substrate. Thus, we expect this technique to provide us with means to prepare carbon nanotube electrodes for device applications such as super capacitors, thermo-electric devices, fuel cells, and field emission filaments.
Contact Damage Accumulation in Tic 3 SiC 2 (1998)

The evolution of deformation—;microfracture damage below Hertzian contacts in a coarse‐grain Ti 3 SiC 2 is studied. The Hertzian indentation stress—;strain response deviates strongly from linearity beyond a well‐defined maximum, with pronounced strain—;softening, indicating exceptional deformability in this otherwise (elastically) stiff ceramic. Surface and subsurface ceramographic observations reveal extensive quasi‐plastic microdamage zones at the contact sites. These damage zones are made up of multiple intragrain slip and intergrain shear failures, with attendant microfracture at high strains. No ring cracks or other macroscopic cracks are observed on or below the indented surfaces. The results suggest that Ti 3 SiC 2 may be ideally suited to contact applications where high strains and energy absorption prior to failure are required.
Online Prostate-Specific Membrane Antigen and Positron Emission Tomography–Guided Radiation Therapy for Oligometastatic Prostate Cancer (2019)

BgRT plans provided similar PTV coverage and conformity compared with CSABR while incorporating underlying PET activity. These results demonstrate feasibility of BgRT optimization enabling online PSMA-targeted, PET-based tracked dose delivery for OMPC.
Antibacterial Aminoglycosides with a Modified Mode of Binding to the Ribosomal‐RNA Decoding Site (2007)

In dieser Zuschrift wurde der Ring IV in Paromomycin (1) und den folgenden Strukturen falsch wiedergegeben. Die richtige Konfiguration ist hier gezeigt.1
Dealing with Complications (2009)

Learning Objectives Diagnose and manage the complications associated with total hip arthroplasty Learn techniques and skills to minimize the frequency of the complications associated with total hip arthroplasty, including infection, limb-length discrepancy, heterotopic ossification, and neurovascular injuries