gebhart/patrepeval huggingface dataset:
G Protein γ Subunits with Altered Prenylation Sequences Are Properly Modified When Expressed in Sf9 Cells (1996)

The gamma subunits of heterotrimeric G proteins undergo post-translational prenylation and carboxylmethylation after formation of the betagamma dimer, modifications that are essential for alpha-betagamma, betagamma-receptor, and betagamma-effector interactions. We have determined the specific prenyl group present on the beta1gamma1, beta1gamma2, and beta1gamma3 dimers purified from baculovirus-infected Sf9 cells by specific binding to G protein alpha subunits immobilized on agarose. These recombinant dimers undergo the same post-translational modifications determined for gamma1 and gamma2 isolated from mammalian tissues. Furthermore, infection of Sf9 cells with a recombinant baculovirus encoding an alteration of the gamma1 CaaX sequence (gamma1-S74L) resulted in geranylgeranylation of the resulting gamma1 subunit, and alteration of the gamma2 CaaX sequence to CAIS (gamma2-L71S) resulted in farnesylation. Both of these altered gamma subunits were able to associate stably with beta1, and the resulting betagamma dimer bound tightly to alpha-agarose and eluted specifically with aluminum fluoride. These results indicate that Sf9 insect cells properly process the CaaX motif in G protein gamma subunits and are a useful model system to study the role of prenylation in the protein-protein interactions in which the betagamma subunits participate.
ChemInform Abstract: Synthesis of Isoindolo[2,1‐a]indoles by the Palladium‐Catalyzed Annulation of Internal Acetylenes. (2001)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Relationship between Critical Current Density and Self-Field Losses of Ag-Sheathed (Bi,Pb)2Sr2Ca2Cu3Ox Superconducting Tapes (2006)

The critical current of (Bi,Pb)2Sr2Ca2Cu3Ox (Bi‐2223) was varied between 2 A and 120 A using three different approaches: field angle with respect to the wide face of the tape, mechanical strain, and heat treatments. The results of transport loss measurements carried out on the three sets of samples showed that reduced critical current density caused an increase in AC loss as expected from Norris’s model. Comparison of the data among the three sets, however, showed some interesting features in the dependence of the AC loss behavior on the method used to vary critical current density. The results are explained based on the type of defects, localized or distributed, caused by the particular approach used in lowering the critical current.
Photonic Band Gaps and Unusual Photon Transport in Hyperuniform Disordered Structures (2012)

We demonstrate that hyperuniform disordered structures support electromagnetic states with very different transport properties, ranging from Bloch-like modes to diffusive states with characteristic time scales almost two-orders of magnitude larger.
Practical Design of Radiating Part of Post-Wall Waveguide-Fed Parallel Plate Slot Array Antenna by Method of Moments (2020)

A practical design of a radiating part of a post-wall waveguide-fed parallel plate slot array antenna is presented. The radiating part of the antenna is a parallel plate waveguide with an array of radiating slot pairs. The slot pairs are designed by the method of moments (MoM). In the MoM analysis, unknown equivalent magnetic currents on slot apertures are expanded with entire-domain sinusoidal basis functions. The dependence of accuracy and computation time on the number of basis functions is evaluated. The MoM with an appropriate number of basis functions enables accurate and fast analyses. As an example, an efficient design of an array consisting of 21 slot pairs in the longitudinal direction of the parallel plate waveguide is demonstrated.
2PT121 Cooperative Motion Analysis of group II chaperonin at Single-molecule Level using Diffracted X-ray Tracking(The 50th Annual Meeting of the Biophysical Society of Japan) (2012)

General IncorporatedAssociation prepared seveTal truncated SptPs and SicP and exarnined whether the truncated SptPs interact with SicP in vitro by Native-PAGE and western b]otting as the first step.We constructed pET3c-based plasrnids expressing SptPi.i36,SptPi.ios, and SptPi.s6, and the three kind$ ofthe truncated SptPs wcre individually prepared under denaturing conditions, We a]so constructed pET3c-based SicP expression plasmid and prepared SicP under native cenditions.Thus, we prepared the truncated SptPs and SicP individually, and then we examined whether the truncated SptPs interact with SicP.In this meeting, we wi]t show the resutts and discuss further effbrts.
Three-Qubit Randomized Benchmarking (2019)

As quantum circuits increase in size, it is critical to establish scalable multiqubit fidelity metrics. Here we investigate, for the first time, three-qubit randomized benchmarking (RB) on a quantum device consisting of three fixed-frequency transmon qubits with pairwise microwave-activated interactions (cross-resonance). We measure a three-qubit error per Clifford of 0.106 for all-to-all gate connectivity and 0.207 for linear gate connectivity. Furthermore, by introducing mixed dimensionality simultaneous RB-simultaneous one- and two-qubit RB-we show that the three-qubit errors can be predicted from the one- and two-qubit errors. However, by introducing certain coherent errors to the gates, we can increase the three-qubit error to 0.302, an increase that is not predicted by a proportionate increase in the one- and two-qubit errors from simultaneous RB. This demonstrates the importance of multiqubit metrics, such as three-qubit RB, on evaluating overall device performance.
Multiplexing Gain of Capped VBR Video (2005)

CBR (Constant Bit Rate) video fluctuates in quality, while its multiplexing behaviour is easy to predict: the links can be loaded to almost 100%. VBR (Variable Bit Rate) video aims at constant quality, but as the bit rate fluctuates over time the links cannot be fully loaded. Because in unconstrained VBR video the bit rate fluctuation might be too large, capped VBR video is proposed as an alternative. Similarly as for unconstrained VBR video, capped VBR video aims at a constant quality, but when in certain intervals this requires a too high bit rate, the bit rate is limited (i.e., capped) in order to support more video flows on the links, at the expense of a quality reduction.
Development of a Cell Sheet Transportation Technique for Regenerative Medicine (2013)

Purpose: A transportation technique for cell sheets is necessary to standardize regenerative medicine. The aim of this article is to develop and evaluate a new transportation technique for cell sheets. Material and Methods: We developed a transportation container with three basic functions: the maintenance of interior temperature, air pressure, and sterility. The interior temperature and air pressure were monitored by a recorder. Human oral mucosal epithelial cells obtained from two healthy volunteers were cultured on temperature-responsive culture dishes. The epithelial cell sheets were transported via an airplane between the Osaka University and Tohoku University using the developed cell transportation container. Histological and immunohistochemical analyses and flow cytometric analyses for cell viability and cell purity were performed for the cell sheets before and 12 h after transportation to assess the influence of transportation on the cell sheets. Sterility tests and screening for endotoxin and mycoplasma in the cell sheets were performed before and after transportation. Results: During transportation via an airplane, the temperature inside the container was maintained above 32°C, and the changes in air pressure remained within 10 hPa. The cell sheets were well stratified and successfully harvested before and after transportation. The expression patterns of keratin 3/76, p63, and MUC16 were equivalent before and after transportation. However, the expression of ZO-1 in the cell sheet after transportation was slightly weaker than that before transportation. The cell viability was 72.0% before transportation and 77.3% after transportation. The epithelial purity was 94.6% before transportation and 87.9% after transportation. Sterility tests and screening for endotoxin and mycoplasma were negative for all cell sheets. Conclusion: The newly developed transportation technique for air travel is essential technology for regenerative medicine and promotes the standardization and spread of regenerative therapies.
Effect of reduced versus usual lipid emulsion dosing on bilirubin neurotoxicity and neurodevelopmental impairment in extremely preterm infants: study protocol for a randomized controlled trial (2023)

Clinical Trials.gov, NCT04584983, Registered 14 October 2020, https://clinicaltrials.gov/ct2/show/NCT04584983 Protocol version: Version 3.2 (10/5/2022).
A randomized, placebo‐controlled trial of cenicriviroc for treatment of nonalcoholic steatohepatitis with fibrosis (2017)

After 1 year of CVC treatment, twice as many subjects achieved improvement in fibrosis and no worsening of SH compared with placebo. Given the urgent need to develop antifibrotic therapies in NASH, these findings warrant phase 3 evaluation. (Hepatology 2018;67:1754-1767).
Software-improved range resolution for oceanographic HF FMCW radar (2013)

The high-frequency (HF) oceanographic radar system located at the coast is well-known as a tool for synoptic on-line mapping. Recently oceanographic radar systems have been officially recognized by the International Telecommunication Union (ITU) to have their own frequency bands. However, the assigned spectral bandwidth for radar signals is quite narrow. It leads to loosing the required range resolution. To avoid this disadvantage new high-resolution processing techniques can be applied. The signal processing sequence is considered in order to improve target range resolution by simple software modification. A test example of high-resolution technique for range processing is shown using measured signals of oceanographic HF frequency modulated continuous wave (FMCW) radar. © 2013 German Inst of Navigation.
ChemInform Abstract: Group 4 ansa‐Cyclopentadienyl‐Amido Catalysts for Olefin Polymerization (1999)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Bilirubin Removal by Plasmafiltration-Adsorption: Ex vivo Adsorption Kinetics Model and Single Case Report (2022)

Our findings provide the first assessment of bilirubin adsorption in an ex vivo model of plasma perfusion and can be used to design interventional studies in humans, providing guidance for an adequate prescription of treatment frequency and duration.
Shear‐Stress‐Mediated Refolding of Proteins from Aggregates and Inclusion Bodies (2015)

Recombinant protein overexpression of large proteins in bacteria often results in insoluble and misfolded proteins directed to inclusion bodies. We report the application of shear stress in micrometer-wide, thin fluid films to refold boiled hen egg white lysozyme, recombinant hen egg white lysozyme, and recombinant caveolin-1. Furthermore, the approach allowed refolding of a much larger protein, cAMP-dependent protein kinase A (PKA). The reported methods require only minutes, which is more than 100 times faster than conventional overnight dialysis. This rapid refolding technique could significantly shorten times, lower costs, and reduce waste streams associated with protein expression for a wide range of industrial and research applications.
Design of high capacity 3D print codes with visual cues aiming for robustness to the PS channel and external distortions (2009)

Adding high-density information to printed materials enables and improves interesting hardcopy document applications involving security, authentication, physical-electronic round tripping, item-level tagging, and consumer/product interaction. This investigation of robust and high capacity print codes aims to maximize information payload in a given printed page area, subject to robustness to channel errors including distortions introduced by the printing and scanning processes and also due to the usual degradations introduced by user manipulation of printed documents. The novel approach includes statistical print-and-scan channel characterization, designing of robust segmentation using visual cues, unsupervised Bayesian color classification with expectation-maximization algorithm for parameters estimation of a mixture of Gaussians model and design of error correction codes. Results illustrate the performance evaluated under real channel and distortions conditions. High payload is achieved with sufficient robustness to distortions resulting of regular office hardcopy document handling: print-and-scan channel and user manipulation.
[Therapy of banal HSV lesions: molecular mechanisms of the antiviral activity of zinc sulfate]. (1991)

Preparations of heparin in combination with the physiologically harmless and even beneficial zinc sulphate are available for the topical treatment of superficial mucocutaneous lesions caused by HSV (herpes simplex virus). We study the molecular mechanism of the antiviral effects of zinc ions. A concentration of 100 mumol/l Zn2+ in the culture medium reduces the virus yield in a HSV infected AGMK cell line to less than 1/1000 of the control. At this concentration zinc sulphate does not exert any major cytotoxic effects, nor does it block the synthesis of viral or cellular DNA. Free virus, however, is inactivated by 8 orders of magnitude by 15 mmol/l zinc sulphate within a few hours. The inactivated virus is limited in the glycoprotein-dependent functions adsorption and penetration. Electron micrographs show massive deposition of zinc onto virion components. The dramatic antiviral effect in vivo is therefore explained by an inhibition of virion glycoprotein functions after accumulation of zinc in the virion, presumably by binding to sulphhydryl groups of glycoprotein B.
Development of hybrid NIR/MRI imaging system algorithm: use of a-priori information for tumor detection in the female breast (2003)

The use of dual modality MRI and near infrared (NIR) optical tomography is under investigation. It will be shown in this work that accurate structural a-priori knowledge from the MRI image, together with appropriate constraints on the image reconstruction can yield useful and accurate images of internal optical property distributions and that this should help in identifying anomalies within the breast tissue.
Research in site-specific propagation modeling for PCS system design (2002)

Some of the work being done for site-specific propagation modeling at the Virginia Tech Mobile and Portable Radio Research Group (MPRG) is described. Ray tracing and diffraction propagation models that utilize site-specific building information are used to predict path loss and multipath power delay profiles.
Randomized Routing on Fat-Trees (1989)

Fat-trees are a class of routing networks for hardware-efficient parallel computation. This paper presents a randomized algorithm for routing messages on a fat-tree. The quality of the algorithm is measured in terms of the load factor of a set of messages to be routed, which is a lower bound on the time required to deliver the messages. We show that if a set of messages has load factor on a fat-tree with n processors, the number of delivery cycles (routing attempts) that the algorithm requires is O(+lg n lg lg n) with probability 1 \\Gamma O(1=n). The best previous bound was O( lg n) for the off-line problem in which the set of messages is known in advance. In the context of a VLSI model that equates hardware cost with physical volume, the routing algorithm can be used to demonstrate that fat-trees are universal routing networks. Specifically, we prove that any routing network can be efficiently simulated by a fat-tree of comparable hardware cost. 1 Introduction Fat-trees constitute...
Vision Guided Biped Walking: Step Sequence Planning, Visual Perception and Feedback (2002)

This paper presents key ideas of our approach to perception guided robot walking. Results of computer vision are employed for reactive step sequence adaptation. Our approach and the guidance system were tested and evaluated in experiments with the biped robot BARt-UH developed at IRT of the Universitat Hannover.
Effects of Medical Research on Health Care and the Economy (1999)

Rapidly expanding federal health care costs of an aging population will not be contained by managed care, capping or similar strategies; rather, the problem requires an understanding of the dynamic relationship of new health technologies to the overall economy. Seven factors, each related to biotechnological innovation, will combine to alter medical knowledge and practice, outcomes and costs in the coming decades, producing a multiplier effect on the economy that may well control or offset future costs. Investing in biomedical research will be key to maintaining the solvency of Medicare.
[Discussion on "dry needling" being part of acupuncture]. (2017)

We think that all the methods of puncturing into the skin to prevent and treat diseases are belong to acupuncture science. In spite of its basic theory of meridian and acupoint, anatomy and physiology have been important parts of modern acupuncture science. "Dry needling", however, is limited to trigger point theory. As for the positions, acupuncture is applied mainly at acupoints, involving in skin, muscles, tendons, vessels and nerves; while "dry needling" is used mostly at muscles. The needles of acupuncture are in various lengths and diameters and its manipulations are abundant, including the traditional skills and the achievements of modern science and technology research, such as electroacupuncture. It is different from the "dry needling" with the single tool and manipulation. Thus, acupuncture is suitable for a large range of syndromes, but "dry needling" is mainly for fascia muscularis pain and other related disorders. The acupuncturists need to embrace Chinese and western medicine, which is more rigorous than the training for "dry needling" practitioners. Based on the above reasons, we consider "dry needling" as part of acupuncture science, and it is a method during the modern development of traditional acupuncture.
Architecture-Dependent Interplay between Self-Assembly and Crystallization in Discrete Block Co-Oligomers (2019)

Access to versatile and stable nanostructures formed by the self-assembly of block copolymers in water is essential for biomedical applications. These applications require control over the stability, morphology, and size of the formed nanostructures. Here, we study the self-assembly in water of a library of fully discrete and sequence-controlled AB-type block co-oligomers (BCOs) of oligo(l-lactic acid)-<i>b</i>-oligo(ethylene glycol). In this series, we eliminate all the inherent uncertainty associated with molar mass, ratio, and compositional dispersity, but vary the ratio between the water-soluble and water-insoluble parts. The BCO library is designed in such a way that vesicles, spherical micelles, and cylindrical micelles are generated in solution, hereby covering a variety of common morphologies. With the help of self-consistent field (SCF) computations, the thermodynamic structures in water are predicted for all structures. The morphologies formed were experimentally analyzed using a combination of calorimetry and scattering techniques. When comparing the experimentally found structures with those predicted, we find an excellent agreement. Intriguingly, calorimetry showed the presence of crystallized l-lactic acid (LLA) units in the bilayer of the lamellar forming BCO. Despite this crystallinity, there is no mismatch between the predicted and observed bilayer thicknesses upon self-assembly in water. In this case, phase separation driven by the hydrophobic LLA block coincides with crystallization, resulting in stable morphologies. Thus, SCF guided library design and sample preparation can lead toward robust formulations of nanoparticles.
TIE-2-expressing monocytes are lymphangiogenic and associate specifically with lymphatics of human breast cancer (2015)

In experimental mouse models of cancer, increasingly compelling evidence point toward a contribution of tumor associated macrophages (TAM) to tumor lymphangiogenesis. Corresponding experimental observations in human cancer remain scarce although lymphatic metastasis is widely recognized as a predominant route for tumor spread. We previously showed that, in malignant tumors of untreated breast cancer (BC) patients, TIE-2-expressing monocytes (TEM) are highly proangiogenic immunosuppressive cells and that TIE-2 and VEGFR signaling pathways drive TEM immunosuppressive function. We report here that, in human BC, TEM express the canonical lymphatic markers LYVE-1, Podoplanin, VEGFR-3 and PROX-1. Critically, both TEM acquisition of lymphatic markers and insertion into lymphatic vessels were observed in tumors but not in adjacent non-neoplastic tissues, suggesting that the tumor microenvironment shapes both TEM phenotype and spatial distribution. We assessed the lymphangiogenic activity of TEM isolated from dissociated primary breast tumors <i>in vitro</i> and <i>in vivo</i> using endothelial cells (EC) sprouting assay and corneal vascularization assay, respectively. We show that, in addition to their known hemangiogenic function, TEM isolated from breast tumor display a lymphangiogenic activity. Importantly, TIE-2 and VEGFR pathways display variable contributions to TEM angiogenic and lymphangiogenic activities across BC patients; however, combination of TIE-2 and VEGFR kinase inhibitors abrogated these activities and overcame inter-patient variability. These results highlight the direct contribution of tumor TEM to the breast tumor lymphatic network and suggest a combined use of TIE-2 and VEGFR kinase inhibitors as a therapeutic approach to block hem- and lymphangiogenesis in BC.
Monolithic optoelectronic switch based on the integration of a GaAs/AlGaAs heterojunction bipolar transistor and a GaAs vertical-cavity surface-emitting laser (1993)

We describe the design and the first experimental demonstration of a monolithic integrated optoelectronic switch combining a GaAs/AlGaAs heterojunction bipolar transistor (HBT) with a vertical-cavity surface-emitting laser (VCSEL). The switch has high current gain (500-700), low power dissipation (27 and 55 mW DC at optical output levels of 0.4 and 1.2 mW, respectively), and a high optical-to-electrical conversion efficiency (150 W/A) under DC bias conditions, thus providing a high-performance electrical-to-optical interface for high-speed optical interconnections.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>