gebhart/patrepeval huggingface dataset:
Keratinocyte Growth Factor Reduces Lung Damage Due to Acid Instillation in Rats (1996)

Abstract Acid aspiration is a serious complication of anesthesia and other forms of unconsciousness that can result in the adult respiratory distress syndrome (ARDS), which continues to have a very high mortality despite our current therapeutic interventions. This type of injury damages the alveolar epithelium, principally alveolar type I cells, and requires proliferation of alveolar type II cells to restore gas exchange units. Since keratinocyte growth factor (KGF) has been shown to be a potent mitogen for alveolar type II cells, we evaluated whether intrabronchial administration of KGF would minimize lung injury due to the unilateral instillation of 0.1 N hydrochloric acid (HCl). Rats were pretreated or post-treated by intrabronchial instillation of KGF (5 mg/kg) into the left lung before HCl instillation. All rats receiving KGF at 48 or 72 h before HCl instillation survived for the 7-day observation period, whereas the mortality rate for those receiving HCl alone or saline followed by HCl was 31% and 33%, respectively. Pretreatment with KGF at 72 h but not at 24 or 48 h considerably ameliorated morphologic damage produced by HCl. Inflammatory cells in bronchoalveolar lavage were markedly decreased 3 and 7 days after HCl instillation by the 72-h KGF pretreatment. Pretreatment with KGF at 72 h also attenuated the reduction of total lung capacity, decreased the α1(I) procollagen mRNA levels, and diminished hydroxyproline accumulation due to HCl instillation. Saline pretreatment at 72 h had no significant effect on the HCl injury and subsequent physiologic abnormalities. Our attempts to improve outcome with post-treatment instillation of KGF were unsuccessful. We conclude that KGF pretreatment reduces lung injury due to acid instillation and can prevent subsequent pulmonary fibrosis.
Release ofHelicobacter pylori vacuolating cytotoxin by both a specific secretion pathway and budding of outer membrane vesicles. Uptake of released toxin and vesicles by gastric epithelium (1999)

The mechanisms by which Helicobacter pylori releases its virulence factors are poorly known. Active secretion has been proposed for some products, including a vacuolating toxin (VacA). Outer membrane vesicles represent another mechanism by which some Gram-negative bacteria may release virulence factors. This study sought to localize VacA by immunocytochemistry in H. pylori cells, to determine whether H. pylori produces outer membrane vesicles, and to investigate whether such vesicles might constitute a vehicle for the delivery of bacterial virulence factors to the gastric mucosa. Small (50-300 nm) membrane vesicles were found in H. pylori culture media from both H. pylori strain 60190 and strain CCUG 17874. These vesicles appeared to originate from blebs arising on the bacterial outer membrane. VacA was immunolocalized in the periplasm and outer membrane of intact bacteria and also in outer membrane blebs and vesicles. Both soluble secreted VacA and VacA-containing vesicles bound to, and were internalized by, MKN28 cells and were detectable in the gastric mucosa from H. pylori-infected humans. The release of outer membrane vesicles by H. pylori may represent a mechanism, additional to secretory pathways, for the delivery of bacterial toxins and antigens to the gastric mucosa.
Experimental investigation of air heated bubble column humidification dehumidification desalination system (2020)

A novel configuration of humidification dehumidification (HDH) desalination system is presented using direct contact bubble column (BC) humidifier and dehumidifier. Experiments are carried out for performance analysis and comparison between a single-stage, HDH air heated system (one humidifier and a dehumidifier) and a double-stage HDH system (two BC humidifiers with air reheating). Effect of various operating parameters such as airflow rate, air temperature, and humidifier water levels is studied on performance metrics such as productivity, gain output ratio (GOR), input energy, specific energy consumption, and effectiveness. Results show that higher airflow rate, air temperature, and humidifier water level favor the system performance. The single-stage system has a maximum productivity rate of 0.19 L/h and GOR of 0.21 obtained at 140°C and 1.5 standard cubic feet per minute (SCFM). However, the productivity of the single-stage HDH system is limited due to low air outlet temperature at the exit of the BC humidifier. The double-stage humidification system performs better in terms of productivity and GOR. The two-stage humidification HDH system produced a distillate rate of 0.46 L/h and GOR of 0.4 with thermal energy increment of only 0.27 kW compared to the single-stage system. The obtained results are promising in comparison with previous HDH studies, especially of air heated cycle. The estimated cost of production is 0.090 USD/L for double-stage HDH BC.
Dasatinib (BMS-354825), a novel multi-targeted kinase inhibitor that blocks tumor cell migration and invasion, is a promising therapeutic agent for metastatic prostate cancer (2006)

Proc Amer Assoc Cancer Res, Volume 47, 2006

3797 

SRC family kinases (SFKs) are currently being investigated as targets for treatment strategies in various cancers. Dasatinib (BMS-354825) is a novel, highly potent, oral, multi-targeted kinase inhibitor of BCR-ABL and SFKs. Dasatinib has been shown to inhibit growth of BCR-ABL-dependent chronic myeloid leukemia xenografts in nude mice and has been efficacious and well tolerated in a Phase I clinical trial of patients with imatinib-resistant or -intolerant CML. Dasatinib has also demonstrated activity against cultured human prostate and breast cancer cells; however, the molecular mechanism by which dasatinib acts on epithelial tumor cells remains unknown. In this study, we show that dasatinib blocks the kinase activities of the SFKs, Lyn and Src in human prostate cancer cells at low nanomolar concentrations. Moreover, focal adhesion kinase (FAK), which has been shown to be elevated in epithelial tumors, and Crk-associated substrate (p130CAS) signaling downstream of SFKs are also inhibited at similar dasatinib concentrations. Consistent with inhibition of these signaling pathways, dasatinib suppresses cell adhesion, migration, and invasion of prostate cancer cells at low nanomolar concentrations. Therefore, dasatinib has potential as a therapeutic agent for metastatic prostate cancers harboring activated SFK and FAK signaling. Phase I clinical evaluation of dasatinib in a variety of solid tumors, including prostate cancer, is currently ongoing.
Cerebral Hemodynamic and White Matter Changes of Type 2 Diabetes Revealed by Multi-TI Arterial Spin Labeling and Double Inversion Recovery Sequence (2017)

Diabetes has been reported to affect the microvasculature and lead to cerebral small vessel disease (SVD). Past studies using arterial spin labeling (ASL) at single post-labeling delay reported reduced cerebral blood flow (CBF) in patients with type 2 diabetes. The purpose of this study was to characterize cerebral hemodynamic changes of type 2 diabetes using a multi-inversion-time 3D GRASE pulsed ASL (PASL) sequence to simultaneously measure CBF and bolus arrival time (BAT). Thirty-six patients with type 2 diabetes (43-71 years, 17 male) and 36 gender- and age-matched control subjects underwent MRI scans at 3 T. Mean CBF/BAT values were computed for gray and white matter (GM and WM) of each subject, while a voxel-wise analysis was performed for comparison of regional CBF and BAT between the two groups. In addition, white matter hyperintensities (WMHs) were detected by a double inversion recovery (DIR) sequence with relatively high sensitivity and spatial resolution. Mean CBF of the WM, but not GM, of the diabetes group was significantly lower than that of the control group (<i>p</i> < 0.0001). Regional CBF decreases were detected in the left middle occipital gyrus (<i>p</i> = 0.0075), but failed to reach significance after correction of partial volume effects. BAT increases were observed in the right calcarine fissure (<i>p</i> < 0.0001), left middle occipital gyrus (<i>p</i> < 0.0001), and right middle occipital gyrus (<i>p</i> = 0.0011). Within the group of diabetic patients, BAT in the right middle occipital gyrus was positively correlated with the disease duration (<i>r</i> = 0.501, <i>p</i> = 0.002), BAT in the left middle occipital gyrus was negatively correlated with the binocular visual acuity (<i>r</i> = -0.408, <i>p</i> = 0.014). Diabetic patients also had more WMHs than the control group (<i>p</i> = 0.0039). Significant differences in CBF, BAT, and more WMHs were observed in patients with diabetes, which may be related to impaired vision and risk of SVD of type 2 diabetes.
Spectroscopic Indications of Tunnel Barrier Charging as the Switching Mechanism in Memristive Devices (2017)

Abstract Resistive random access memory is a promising, energy‐efficient, low‐power “storage class memory” technology that has the potential to replace both flash storage and on‐chip dynamic memory. While the most widely employed systems exhibit filamentary resistive switching, interface‐type switching systems based on a tunable tunnel barrier are of increasing interest. They suffer less from the variability induced by the stochastic filament formation process and the choice of the tunnel barrier thickness offers the possibility to adapt the memory device current to the given circuit requirements. Heterostructures consisting of a yttria‐stabilized zirconia (YSZ) tunnel barrier and a praseodymium calcium manganite (PCMO) layer are employed. Instead of spatially localized filaments, the resistive switching process occurs underneath the whole electrode. By employing a combination of electrical measurements, in operando hard X‐ray photoelectron spectroscopy and electron energy loss spectroscopy, it is revealed that an exchange of oxygen ions between PCMO and YSZ causes an electrostatic modulation of the effective height of the YSZ tunnel barrier and is thereby the underlying mechanism for resistive switching in these devices.
Characterization of RNA primers synthesized by the human breast cancer cell DNA synthesome (2009)

We previously reported on the purification and characterization of a functional multi-protein DNA replication complex (the DNA synthesome) from human cells and tissues. The synthesome is fully competent to carry-out all phases of the DNA replication process in vitro. In this study, DNA primase, a component of the synthesome, is examined to determine its activity and processivity in the in vitro synthesis and extension of RNA primers. Our results show that primase activity in the P4 fraction of the synthesome is 30-fold higher than that of crude cell extracts. The synthesome synthesizes RNA primers that are 7-10 ribonucleotides long and DNA primers that are 20-40 deoxyribonucleotides long using a poly(dT) template of exogenous single-stranded DNA. The synthesome-catalyzed RNA primers can be elongated by E. coli DNA polymerase I to form the complementary DNA strands on the poly(dT) template. In addition, the synthesome also supports the synthesis of native RNA primers in vitro using an endogenous supercoiled double-stranded DNA template. Gel analysis demonstrates that native RNA primers are oligoribonucleotides of 10-20 nt in length and the primers are covalently link to DNA to form RNA-primed nascent DNA of 100-200 nt. Our study reveals that the synthesome model is capable of priming and continuing DNA replication. The ability of the synthesome to synthesize and extend RNA primers in vitro elucidates the organizational and functional properties of the synthesome as a potentially useful replication apparatus to study the function of primase and the interaction of primase with other replication proteins.
Plain-pattern to cross-section transfer (PCT) technique for deep x-ray lithography and applications (2004)

This paper presents a novel fabrication method for three-dimensional microstructures using deep x-ray lithography. The microstructures were fabricated including sloped sidewalls and curved surfaces by exposing a synchrotron radiation beam to a moving x-ray resist. The technique, the so-called plain-pattern to cross-section transfer (PCT) technique, has been developed as an extension to conventional 2.5-dimension lithography. The fabrication of PMMA microstructures has been demonstrated with surfaces as smooth as 10 nm of RMS roughness. Various applications, e.g., micro-optic, bio-medical and some components of MEMS devices have been realized. Two microstructures have been given as examples: microlens arrays and microneedle arrays. The resulting arrays can be employed to fabricate moulds by electro-deposition for further batch-processing using the LIGA process.
The behavior of carboxylated and hydroxylated polythiophene as bioreceptor layer: Anti‐human IgG and human IgG interaction detection based on organic electrochemical transistors (2021)

Abstract The sensing properties of poly 3‐(3‐carboxypropyl) thiophene‐2,5‐diyl (PT‐COOH) and hydroxylated polythiophene (PT‐OH) as bioreceptor layers were studied and are discussed in this paper. The polymer films cover the channel region of the OECT devices and anti‐human IgG was immobilized on the polymer films. We use threshold voltage (Vth) change as a sensing signal to detect the interaction between anti‐human IgG and human IgG. By adding different concentrations of human IgG, Vth difference can be observed on anti‐human IgG immobilized polymer films, with optimized detection from a blend of the two polymers. Open circuit potential (OCP) measurement was also done on the OECT devices based on the same anti‐human IgG and human IgG interaction pair to help us understand the mechanism behind the antibody functionalization and the interaction between antibody and antigen. Importantly, the observed positive OCP change for the PT‐OH system was self‐consistent with the negative OECT Vth change that was obtained, since the latter is applied to the gate while the former is measured at the channel.
Carbon nanotube formation in situ during carbonization in shaped bulk solid cobalt nanoparticle compositions (2004)

Carbon nanotubes are formed in bulk compositions from thermal decomposition of a cobalt-containing organometallic compound in the presence of an excess amount of a multi(ethynyl)aromatic compound. The novel method described here permits the large-scale production of carbon nanotubes in a shaped, solid configuration. 1,2,4,5-Tetrakis(phenylethynyl)benzene was reacted with varying amounts of cobalt octacarbonyl to produce the cobalt carbonyl complex. Heat treatment of various compositions, formulated from the cobalt complex and an excess amount of 1,2,4,5-tetrakis(phenylethynyl)benzene, to temperatures up to 1400 °C resulted in the decomposition of the complex and the formation of cobalt nanoparticles in the polymeric-to-carbon nanoparticle-to-carbon nanotube compositions. The cobalt nanoparticles are the key to the formation of the carbon nanotubes. X-ray diffraction, Raman spectroscopy, high resolution scanning electron microscopy (HRSEM), and transmission electron microscopy (TEM) studies show the formation of carbon nanotubes in the developing carbon composition above 600 °C.
Evidence for vesicles that mediate long-chain fatty acid uptake by human microvascular endothelial cells (2002)

This study analyzes the mechanisms of long-chain fatty acid (LCFA) uptake by human microvascular endothelial cells (HMEC). The time course revealed the presence of an early, carrier-mediated uptake component and a later component mediated by clathrin-coated vesicles (CCV) and caveolae, as evidenced by three different experimental approaches: 1) significant reduction of [3H]oleate uptake over 5 min by either inhibition of CCV formation by potassium depletion or hypertonic medium, or disruption of caveolae by filipin III or cyclodextrin. 2) Co-localization of intracellular 12-(N-methyl)-N-[(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]octadecanoic acid with CCV and caveolae using confocal laser scanning microscopy. 3) Enrichment of [3H]oleate in a subcellular fraction containing CCV and caveolae. Within 10 min, more than 75% of intracellular [3H]oleate remained unmetabolized, suggesting that HMEC preferentially shuttle LCFA through the cell using CCV and caveolae as carriers. The uptake of albumin paralleled that of oleate within the first 10 min, suggesting internalization of at least some LCFA bound to albumin. Compared to oleate and albumin, the uptake of sucrose and dextran was low, indicating a potential minor contribution of fluid-phase endocytosis to the total vesicular LCFA uptake. The data indicate a previously unrecognized role of both CCV and caveolae for the uptake of LCFA by HMEC.
Simulation and Control of Deformable Autonomous Airships in Turbulent Wind (2022)

Abstract Fixed wing and multirotor UAVs are common in the field of robotics. Solutions for simulation and control of these vehicles are ubiquitous. This is not the case for airships, a simulation of which needs to address unique properties, i) dynamic deformation in response to aerodynamic and control forces, ii) high susceptibility to wind and turbulence at low airspeed, iii) high variability in airship designs regarding placement, direction and vectoring of thrusters and control surfaces. We present a flexible framework for modeling, simulation and control of airships. It is based on Robot operating system (ROS), simulation environment (Gazebo) and commercial off the shelf (COTS) electronics, all of which are open source. Based on simulated wind and deformation, we predict substantial effects on controllability which are verified in real-world flight experiments. All our code is shared as open source, for the benefit of the community and to facilitate lighter-than-air vehicle (LTAV) research. (Source code: https://github.com/robot-perception-group/airship_simulation .)
A Non-Human Primate Model for Gluten Sensitivity (2008)

Gluten-sensitive rhesus macaques may be an attractive resource for investigating both the pathogenesis and the treatment of celiac disease.
Angular analysis and branching fraction measurement of the decay B0→K⁎0μ+μ− (2013)

The angular distributions and the differential branching fraction of the decay B0→K⁎(892)0μ+μ− are studied using a data sample corresponding to an integrated luminosity of 5.2fb−1 collected with the CMS detector at the LHC in pp collisions at s=7TeV. From more than 400 signal decays, the forward–backward asymmetry of the muons, the K⁎(892)0 longitudinal polarization fraction, and the differential branching fraction are determined as a function of the square of the dimuon invariant mass. The measurements are in good agreement with standard model predictions.
An adaptive transmission scheme for multiuser MIMO broadcast channel in Cognitive Radio (2014)

Cognitive Radio (CR) technology provides various options of spectral efficiency for unlicensed wireless communication devices to adapt their spectrum use in response to their operating environment. However, there is an obvious room for improvement regarding the data transmission efficiency and co-existence of secondary users (SUs) in underlay CR. In this paper, we introduce a novel adaptive transmission scheme for multiuser broadcast channel in underlay CR. The proposed scheme applies, on the one hand, a modified water filling algorithm to the primary link ensuring a steady transmission for SUs and, on the other hand, a precoding approach based on the interference alignment and null-steering algorithms to the secondary link enabling coexistence in multiuser underlay CR without constraints on the secondary transmit power. Simulation results show that utilizing this scheme both primary and secondary links can adapt their transmission to commonly improve the spectrum efficiency. Although this comes at the cost of capacity loss in the primary link at high SNRs, it is tolerable as long as a given quality of service for the primary link remains unaffected. In addition, the proposed scheme provides a good performance in terms of BER.
A musculoskeletal ultrasound program as an intervention to improve disease modifying anti-rheumatic drugs adherence in rheumatoid arthritis: a randomized controlled trial (2021)

<b>Objectives</b>: To evaluate the effect of a musculoskeletal ultrasound programme (MUSP) applying real-time ultrasonography with reinforcement of findings by a rheumatologist on improving disease-modifying anti-rheumatic drugs (DMARDs) adherence in rheumatoid arthritis (RA).<b>Method</b>: Eligible RA patients with low adherence score (< 6) on the 8-item Morisky Medication Adherence Scale (MMAS-8) were randomized to either an intervention group (receiving MUSP at baseline) or a control group (no MUSP), and followed up for 6 months. Adherence measures (patient-reported and pharmacy dispensing records) and clinical efficacy data were collected. The MUSP's feasibility and acceptability were assessed.<b>Results</b>: Among 132 recruited RA patients, six without baseline visits were excluded; therefore, 126 patients were analysed (62 intervention and 64 control). The primary outcome (proportion of patients with 1 month MMAS-8 score < 6) was significantly smaller (p = 0.019) in the intervention (35.48%) than the control group (56.25%). However, 3 and 6 month adherence and clinical efficacy outcomes were not significantly different between the two groups (all p > 0.05). All 62 patients completed the MUSP (mean time taken, 9.2 min), with the majority reporting moderately/very much improved understanding of their joint condition (71%) and the importance of regularly taking their RA medication(s) (79%). Most patients (90.3%) would recommend the MUSP to another RA patient.<b>Conclusions</b>: The MUSP improved RA patients' DMARDs adherence in the short term and was feasible and well accepted by patients. Future studies could evaluate whether repeated feedback using MUSP could help to sustain the improvement in DMARD adherence in RA patients, and whether this may be clinically impactful and cost-effective.
Large-scale audio event discovery in one million YouTube videos (2017)

Internet videos provide a virtually boundless source of audio with a conspicuous lack of localized annotations, presenting an ideal setting for unsupervised methods. With this motivation, we perform an unprecedented exploration into the large-scale discovery of recurring audio events in a diverse corpus of one million YouTube videos (45K hours of audio). Our approach is to apply a streaming, nonparametric clustering algorithm to both spectral features and out-of-domain neural audio embeddings. We use a small portion of manually annotated audio events to quantitatively estimate the intrinsic clustering performance. In addition to providing a useful mechanism for unsupervised active learning, we demonstrate the effectiveness of the discovered audio event clusters in two downstream applications. The first is weakly-supervised learning, where we exploit the association of video-level metadata and cluster occurrences to temporally localize audio events. The second is informative activity detection, an unsupervised method for semantic saliency based on the corpus statistics of the discovered event clusters.
BindingDB Entry 50008862: Identification of potent, selective and orally bioavailable phenyl ((R)-3-phenylpyrrolidin-3-yl)sulfone analogues as RORγt inverse agonists. (2021)

An X-ray crystal structure of one of our previously discovered RORγt inverse agonists bound to the RORγt ligand binding domain revealed that the cyclohexane carboxylic acid group of compound 2 plays a significant role in RORγt binding, forming four hydrogen bonding and ionic interactions with RORγt. SAR studies centered around the cyclohexane carboxylic acid group led to identification of several structurally diverse and more potent compounds, including new carboxylic acid analogues 7 and 20, and cyclic sulfone analogues 34 and 37. Notably, compounds 7 and 20 were found to maintain the desirable pharmacokinetic profile of 2.
Conception de tags RFID sans puce imprimés sur papier par Flexographie (2013)

National audience