gebhart/patrepeval huggingface dataset:
Origin of Human Bone Marrow Fibroblasts (1980)

We investigated the origin of bone marrow fibroblasts in three bone marrow transplant recipients with aplastic anaemia and leukaemia who received grafts from HLA-identical siblings of opposite sex. The patients were conditioned for transplantation with high doses of cytotoxic drugs and 300--1000 rads total body irradiation. After transplantation, bone marrow cells were cultured in T flasks for 3 weeks and the adherent cells were then trypsinized and passaged weekly. After several passages the cells had the typical morphology and growth pattern of fibroblasts. Metaphases from these cells were all of recipient sex type. In contrast, haematopoietic cells and lymphocytes obtained at the same time were of donor sex type. Our findings indicate that the human bone marrow fibroblast is not derived from a precursor common to haemotopoietic cells or lymphocytes. The bone marrow fibroblast appears to be a mesenchymal cell, unrelated to haematopoietic stem cells, which is capable of in vitro proliferation after as much as 1000 rads of total body irradiation.
Persistent Activation of mRNA Translation by Transient Hsp90 Inhibition (2020)

The heat shock protein 90 (Hsp90) chaperone functions as a protein-folding buffer and plays a role promoting the evolution of new heritable traits. To better understand how Hsp90 can affect mRNA translation, we screen more than 1,600 factors involved in mRNA regulation for physical interactions with Hsp90 in human cells. The mRNA binding protein CPEB2 strongly binds Hsp90 via its prion domain. In a yeast model, transient inhibition of Hsp90 results in persistent activation of a CPEB translation reporter even in the absence of exogenous CPEB that persists for 30 generations after the inhibitor is removed. Ribosomal profiling reveals that some endogenous yeast mRNAs, including HAC1, show a persistent change in translation efficiency following transient Hsp90 inhibition. Thus, transient loss of Hsp90 function can promote a nongenetic inheritance of a translational state affecting specific mRNAs, introducing a mechanism by which Hsp90 can promote phenotypic variation.
Single-mode tunable mid-IR laser based on a high-Q silicon microresonator (2020)

We demonstrate a single longitudinal mode, tunable mid-IR laser by self-injection locking a multiple longitudinal mode Interband Cascade Laser (ICL) to a high-Q Si microresonator at 3.4 p,m.
Characterization of transglutaminase type II role in dendritic cell differentiation and function (2010)

DCs play an essential role in the endotoxic shock, and their profound depletion occurs in septic patients and septic mice. TG2(-/-) mice are more resistant to the endotoxic shock induced by LPS. Here, we studied the cellular and molecular basis of this effect, analyzing the role of the enzyme in DC maturation and function. We show that TG2 is up-regulated drastically during the final, functional maturation of DCs consequent to LPS treatment. In keeping with this finding, the inhibition of the enzyme cross-linking activity determines the impairment of DC function highlighted by wide phenotypic changes associated with a reduced production of cytokines (IL-10, IL-12) after LPS treatment and a lower ability to induce IFN-gamma production by naïve T cells. The in vivo analysis of DCs obtained from TG2(-/-) mice confirmed that the enzyme ablation leads to an impairment of DC maturation and their reduced responsiveness to LPS treatment. In fact, a marked decrease in DC death, TLR4 down-regulation, and impaired up-regulation of MHCII and CD86 were observed in TG2(-/-) mice. Taken together, these data suggest that TG2 plays an important role in regulating the response of DCs to LPS and could be a candidate target for treating endotoxin-induced sepsis.
ChemInform Abstract: Organometallic Routes into the Nanorealms of Binary Fe‐Si Phases (2011)

Abstract Review: 115 refs.
A new synchronization concept for solar array deployments (2005)

RESUME Cosmo SkyMed is an Italian scientific space project involving three satellites, each of them equipped with a two-wing solar array. ALCATEL SPACE is in charge of the development, qualification and delivery of these solar arrays, one particularity of which is the deployment kinematics : each wing is deployed on a 180° angular stroke, but the four-panel stack must unfold during the second half only (i.e. from 90° to 180°). The deployment concept is based on a cable synchronization device providing several key advantages and allowing a size similar to usual ALCATEL SPACE solar array deployment mechanisms. 1. OVERALL DESCRIPTION The mechanism used to link the Solar Array Wing (SAW) to the spacecraft and perform its deployment is called the Root Hinge. Fig.1. Solar Array Overview The root hinge ensures : the deployment of the whole solar array from the stowed position to the deployed configuration (0° to 180°), the locking in deployed configuration, the wing interface with the solar array drive mechanism. With the exception of the synchronization device, the parts and components of the root hinge are derived from the mechanisms used on ALCATEL SPACE Spacebus telecom satellites, and adapted to fit the specific Cosmo SkyMed interface requirements. These include : the structural brackets, the electrical motor gear, the latching device, the ball joints (for rotation guidance).
Editorial Comment (1983)

Professor and Chairman Department of Orthopedic Surgery Rush-Presbyterian-St. Luke's Medical Center Chicago, Illinois
Optical characterization of visible multiquantum-well semiconductor lasers by collection/excitation modes of scanning near-field optical microscopy (1999)

Both collection and excitation modes of scanning near-field optical microscopy were used to study a strained AlGaInP/Ga0.4In0.6P low power visible multiquantum-well laser diode. Collection and excitation modes provide the near-field optical propagating intensity distribution and local photoconductivity information, respectively, at the facet of laser diode. Results show highly localized spatial correlation of the laser diode structure and its optical performance at the facet. Defect level in the energy range of 60–380 meV below the conduction band in the highly n-doped (Al0.7Ga0.3)0.5In0.5P cladding layer was found by the excitation mode using the wavelengths of 543.5 and 632.8 nm. Spatially resolved near-field optical spectra of both stimulated and spontaneous emissions were acquired as well. Longitudinal modes of the stimulated emission of laser diode were observed locally.
P16-23. Antigen processing influences HIV-specific cytotoxic T lymphocyte immunodominance (2009)

International audience
Analysis of cell cultures of Taxus wallichiana using direct analysis in real‐time mass spectrometric technique (2007)

The applicability of a new mass spectrometric technique, DART (direct analysis in real time) has been studied in the analysis of the calli of Taxus wallichiana. The intact callus samples were directly analyzed by holding them in the gap between the DART source and mass spectrometer for measurements. Five C-14 oxygenated taxoids were characterized from the analysis of the calli of the Taxus wallichiana almost instantaneously. The confirmation of the structures of the identified taxoids was made through their accurate molecular formula determinations.
Scalable Attestation: A Step toward Secure and Trusted Clouds (2015)

Scalable attestation combines secure boot and trusted boot technologies, and extends them up into the host, its programs, and into the guest's operating system and workloads, to both detect and prevent integrity attacks. Anchored in hardware, this integrity appraisal and attestation protects persistent data (files) from remote attack, even if the attack is root privileged. As an added benefit of a hardware rooted attestation, the authors gain a simple hardware-based geolocation attestation to help enforce regulatory requirements. This design is implemented in multiple cloud testbeds based on the QEMU/KVM hypervisor, OpenStack, and OpenAttestation, and is shown to provide significant additional integrity protection at negligible cost.
Discovery and Structure–Activity Relationship (SAR) of a Series of Ethanolamine-Based Direct-Acting Agonists of Sphingosine-1-phosphate (S1P1) (2016)

Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that regulates a multitude of physiological processes such as lymphocyte trafficking, cardiac function, vascular development, and inflammation. Because of the ability of S1P1 receptor agonists to suppress lymphocyte egress, they have great potential as therapeutic agents in a variety of autoimmune diseases. In this article, the discovery of selective, direct acting S1P1 agonists utilizing an ethanolamine scaffold containing a terminal carboxylic acid is described. Potent S1P1 agonists such as compounds 18a and 19a which have greater than 1000-fold selectivity over S1P3 are described. These compounds efficiently reduce blood lymphocyte counts in rats through 24 h after single doses of 1 and 0.3 mpk, respectively. Pharmacodynamic properties of both compounds are discussed. Compound 19a was further studied in two preclinical models of disease, exhibiting good efficacy in both the rat adjuvant arthritis model (AA) and the mouse experimental autoimmune encephalomyelitis model (EAE).
TCP-Mobile Edge: Accelerating delivery in mobile networks (2012)

Owing to the imminent fixed mobile convergence, Internet applications are frequently accessed through mobile nodes. However, service delivery latency is too high to satisfy user expectations. In this paper, we design a new TCP algorithm, TCP-ME (Mobile Edge), to accelerate the service delivery in mobile networks. Considering the QoS (Quality of Service) mechanisms of mobile networks, TCP-ME is designed to differentiate the packet loss caused by wireless errors, traffic conditioning of mobile core networks, and Internet congestion, as well as to react to the packet loss accordingly. To detect wireless errors, we mark the ACK (Acknowledge) packets in the uplink direction at the base station, and the marking threshold is a function of the instantaneous downlink queue length and the number of consecutive HARQ retransmissions. We modify the ECN mechanism with deterministic marking to detect Internet congestion. The packet loss caused by traffic conditioners of mobile networks is detected by whether the incoming DUPACK is marked or not. TCP-ME adapts the inter-packet interval when the packet loss is caused by wireless errors or the admission control mechanism. If the packet loss is due to Internet congestion, TCP-ME applies the TCP-New Reno's congestion window adaptation algorithm. Simulation results show that TCP-ME can speed up web service response time in mobile networks by about 80%.
Discovery of Potent and Selective 7-Azaindole Isoindolinone-Based PI3Kγ Inhibitors (2020)

The successful application of immunotherapy in the treatment of cancer relies on effective engagement of immune cells in the tumor microenvironment. Phosphoinositide 3-kinase γ (PI3Kγ) is highly expressed in tumor-associated macrophages, and its expression levels are associated with tumor immunosuppression and growth. Selective inhibition of PI3Kγ offers a promising strategy in immuno-oncology, which has led to the development of numerous potent PI3Kγ inhibitors with variable selectivity profiles. To facilitate further investigation of the therapeutic potential of PI3Kγ inhibition, we required a potent and PI3Kγ-selective tool compound with sufficient metabolic stability for use in future <i>in vivo</i> studies. Herein, we describe some of our efforts to realize this goal through the systematic study of SARs within a series of 7-azaindole-based PI3Kγ inhibitors. The large volume of data generated from this study helped guide our subsequent lead optimization efforts and will inform further development of PI3Kγ-selective inhibitors for use in immunomodulation.
DNA methyltransferase levels and altered CpG methylation in the total genome and in the GSTP1 gene in human glioma cells transfected with sense and antisense DNA methyltransferase cDNA (2000)

This study examines the efficacy of using plasmid expression vectors containing sense and antisense DNA MTase cDNA to both up- and downregulate intracellular DNA MTase levels in human glioma cells. The effects of the changes in MTase levels on global genomic DNA methylation and on the methylation status of CpG dinucleotides in the GSTP1 gene were determined in a glioma cell line that overexpresses the GSTP1 gene. In cells transfected with sense DNA MTase cDNA, MTase gene transcripts increased to a maximum of 2.5-fold at 24 h, while MTase activity increased to a maximum of 3.6-fold at 48 h. The effects of antisense MTase cDNA transfections were less pronounced, and levels of MTase gene transcripts and enzyme activity in transfectants were decreased to only, approximately, one-half the levels of controls. The alterations in DNA MTase expression were associated with corresponding changes in the level of global DNA methylation and in the methylation of the GSTP1 gene in the cells, however, with no detectable morphological or cytotoxic effects on the cells. No significant changes in GSTP1 gene expression were detected after the transfections, presumably because of the high levels of basal GSTP1 expression in the cells. Consequently, the p16 gene, known to be repressed transcriptionally by DNA methylation, was examined for the functional effects of the altered MTase levels. The results showed a 2-fold decrease in p16 gene transcripts with the sense MTase transfectants, while in the MTase antisense-transfected cells p16 transcript levels increased by 30%. Together, these results demonstrate the feasibility of using both sense and antisense DNA MTase expression vectors to regulate DNA MTase levels in glioma cells and that, over relatively short periods of time, the alterations in MTase activities are not deleterious to the cells. The system provides a model with which the role of DNA methylation in critical genes and DNA sequences can be investigated in glioma cells. J. Cell. Biochem. 77:372–381, 2000. © 2000 Wiley-Liss, Inc.
7.1: Invited Paper : Progress in Luminous Array Film (LAFi) with Plasma Tube Technology for Seamless Tilling Super‐large‐area Display (2013)

Abstract A newly developed flat sealing process for glass tube edge provided a minimal seam by overlapped connection with transparent tube edge. Using these new techniques, we produced a new model of free expandable 1m × 2m stand‐alone display module that will provide a free designed curved super‐large area display.
Analysis of NKp30/NCR3 isoforms in untreated HIV-1-infected patients from the ANRS SEROCO cohort (2013)

Natural killer (NK) cells play a prominent role at the intersection between innate and cognate immunity, thus influencing the development of multiple pathological conditions including HIV-1-induced AIDS. Not only NK cells directly kill HIV-1-infected cells, but also control the maturation and/or elimination of dendritic cells (DCs). These functions are regulated by the delicate balance between activating and inhibiting receptors expressed at the NK-cell surface. Among the former, NKp30 has raised significant interest since the alternative splicing of its intracellular domain leads to differential effector functions, dictating the prognosis of patients bearing gastrointestinal sarcoma, and B7-H6 has recently been identified as its main ligand. Since NKp30 is downregulated in CD56<sup>-</sup>/CD16<sup>+</sup> NK cells expanded in viremic, chronically infected HIV-1<sup>+</sup> patients, we decided to investigate the predictive value of NKp30 splice variants for spontaneous disease progression in 89 therapy-naïve HIV-1-infected individuals enrolled in an historical cohort of patients followed since diagnosis (ANRS SEROCO cohort). We found no difference in the representation of NK-cell subsets (CD56<sup>bright</sup>, CD56<sup>dim</sup>, CD56<sup>neg</sup>) in HIV-1-infected patients as compared with healthy subjects. NKp30 downregulation was detected in CD56<sup>dim</sup> and CD56<sup>neg</sup> NK-cell subsets, yet this did not convey any prognostic value. None of the NKp30 isoforms did affect disease progression, as measured in terms of time-to-loss of circulating CD4<sup>+</sup> T cells, time-to-AIDS-defining events and overall survival. NKp30 isoforms do not seem to play a major role in the outcome of HIV-1 infection, but the heterogeneity of the immuno-virological status of patients at enrollment could have to be taken into account.
Long-Term Results After Total Knee Arthroplasty with Contemporary Rotating-Hinge Prostheses (2017)

Therapeutic Level IV. See Instructions for Authors for a complete description of levels of evidence.
Sleep stage dynamics in young patients with sleep bruxism (2019)

Sleep stage dynamics differed between SB patients and controls. RMMA does not result in sleep disruption but is likely associated with dissipation of sleep pressure. Less continuity of REM sleep in SB may provide insights into the underlying pathophysiological mechanisms of SB, which may be related to REM sleep processes such as cortical desynchronized states or brainstem activation.
Polymeric conjugates of drugs and antibodies for site‐specific drug delivery (1996)

Abstract The synthesis of targetable conjugates of doxorubicin bound to N‐(2‐hydroxypropyl)methacrylamide copolymers was investigated. Anti‐CD3 antibody against TCR/CD3 complex was used to target the conjugates to T‐cells. The effect of structure of the oligopeptide spacer between the drug and polymer as well as of the polymer modification with the antibody on the rate of drug release from the polymeric carrier system incubated in vitro with cathepsin B or with a mixture of intracellular enzymes (tritosomes) is discussed. The results of in vitro drug‐release experiments are correlated with the evaluation of T‐cell cytotoxicity of targeted and nontargeted polymer‐bound doxorubicin conjugates measured in vitro as the inhibition of Con‐A stimulated growth of human peripheral blood lymphocytes ( 3 H‐thymidine incorporation method).
SARS-CoV-2 Omicron potently neutralized by a novel antibody with unique Spike binding properties (2022)

Abstract The SARS-CoV-2 Omicron variant exhibits very high levels of transmission, pronounced resistance to authorized therapeutic human monoclonal antibodies and reduced sensitivity to vaccine-induced immunity. Here we describe P2G3, a human monoclonal antibody (mAb) isolated from a previously infected and vaccinated donor, which displays picomolar-range neutralizing activity against Omicron BA.1, BA.1.1, BA.2 and all other current variants, and is thus markedly more potent than all authorized or clinically advanced anti-SARS-CoV-2 mAbs. Structural characterization of P2G3 Fab in complex with the Omicron Spike demonstrates unique binding properties to both down and up spike trimer conformations at an epitope that partially overlaps with the receptor-binding domain (RBD), yet is distinct from those bound by all other characterized mAbs. This distinct epitope and angle of attack allows P2G3 to overcome all the Omicron mutations abolishing or impairing neutralization by other anti-SARS-COV-2 mAbs, and P2G3 accordingly confers complete prophylactic protection in the SARS-CoV-2 Omicron monkey challenge model. Finally, although we could isolate in vitro SARS-CoV2 mutants escaping neutralization by P2G3 or by P5C3, a previously described broadly active Class 1 mAb, we found these viruses to be lowly infectious and their key mutations extremely rare in the wild, and we could demonstrate that P2G3/P5C3 efficiently cross-neutralized one another’s escapees. We conclude that this combination of mAbs has great prospects in both the prophylactic and therapeutic settings to protect from Omicron and other VOCs.
ChemInform Abstract: Reactions of Phenols with Thianthrene Cation Radical. (1992)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Combustion Species Sensor for Scramjet Flight Instrumentation (2005)

An in-situ, non-intrusive, compact, and rugged sensor system is been developed for simultaneously monitoring multiple combustion species in scramjets during flight.The sensor is based on diode laser absorption spectroscopy with sensitivity enhanced by wavelength modulation.Simultaneous multi-species concentration measurements are achieved using modulation-frequency multiplexing with several lasers and a single two-color photodiode detector.Measurements of methane, water vapor, and oxygen concentrations, and temperatures were simultaneously measured in a laboratory diffusion flame and in a cavity flameholder with methane injection in a supersonic freestream.No channel cross-talk is observed between the visible and near-infrared layers of the detector and between the laser modulation frequencies. I. Introduction A. MotivationThe development of a scramjet engine to power aircraft to hypersonic speeds has occupied researchers for more than 50 years.Moving from laboratory research to test flight, as evidenced by the successful test flight of NASA's X-43A hypersonic aircraft, sensors are needed during flight to gather information about aerodynamics and combustion.The optimization of engine performance parameters such as combustion efficiency, thrust maximization, reduction in pollutants, and minimization of exhaust signatures (for stealth) are essential.Measurements of chemical species and temperature in the scramjet engine during ground and flight tests are critical for evaluating engine performance.The application of a laser-based optical technique to probe chemical species in a laboratory combustion system has been demonstrated by several research groups [1][2][3][4][5][6][7][8].A laser-based absorption method provides several advantages as compared to methods of probe extraction, laser-induced fluorescence, etc.However, designing a compact and rugged multi-species gas sensor system that is suitable for test flight has yet to be fully explored.The sensor system for monitoring the combustion section of a scramjet during flight must also be nonintrusive and lightweight.A flight-worthy hardware will require substantial reduction in the physical size of current systems, reduction in the number of lasers and detectors, utilization of innovative optics, implementation of compact and stand-alone electronics, and ruggedization of the instrument. B.
Demonstration of multi-Gbps data rates at Ka-band using software-defined modem and broadband high power amplifier for space communications (2011)

Summary form only given, as follows. The paper presents the experimental results regarding the combination of a software-defined multi-Gbps modem and a high power amplifier (HPA) when tested with standard DVB-S2 and LDPC rate 9/10 FEC codec. The modem supports waveforms including QPSK, 8-PSK, 16-APSK, 32-APSK, 64-APSK, & 128-QAM. The broadband HPA is a space qualified TWTA, which has a passband 3 GHz at 33 GHz, output power 200 watts & efficiency 60%. The system enabled an unprecedented data rate at 20 Gbps with BER of 10-9.