gebhart/patrepeval huggingface dataset:
Paxillin and Hydrogen Peroxide-Inducible Clone 5 Expression and Distribution in Control and Alzheimer Disease Hippocampi (2010)

Hydrogen peroxide-inducible clone 5 (Hic-5) and paxillin are members of the Group III LIM domain protein family that localize to both cell nuclei and focal adhesions and link integrin-mediated signaling to the actin cytoskeleton. Prior in vitro studies have implicated paxillin in beta-amyloid-induced cell death, but little is known about the expression and function of Hic-5 and paxillin in the brain. We performed a blinded retrospective cross-sectional study of Hic-5 and paxillin expression in the hippocampi of Alzheimer disease (AD) and control subjects using immunohistochemistry and laser scanning confocal microscopy. The analysis included assessment of the expression of phosphorylated isoforms of paxillin that reflect activation of distinct signaling pathways. We found changes in the subcellular distribution of Hic-5, paxillin, and specific phosphorylated isoforms of paxillin in the AD brains. The Hic-5 and phosphorylated isoforms of paxillin colocalized with neurofibrillary tangles. Paxillin was predominantly found in reactive astrocytes in the AD hippocampi, and activated paxillin was also detected in granulovacuolar degeneration bodies in AD. These data indicate that these important scaffolding proteins that link various intracellular signaling pathways to the extracellular matrix are modified and have altered subcellular distribution in AD.
Development of Standardized Material Testing Protocols for Prosthetic Liners (2017)

A set of protocols was created to characterize prosthetic liners across six clinically relevant material properties. Properties included compressive elasticity, shear elasticity, tensile elasticity, volumetric elasticity, coefficient of friction (CoF), and thermal conductivity. Eighteen prosthetic liners representing the diverse range of commercial products were evaluated to create test procedures that maximized repeatability, minimized error, and provided clinically meaningful results. Shear and tensile elasticity test designs were augmented with finite element analysis (FEA) to optimize specimen geometries. Results showed that because of the wide range of available liner products, the compressive elasticity and tensile elasticity tests required two test maxima; samples were tested until they met either a strain-based or a stress-based maximum, whichever was reached first. The shear and tensile elasticity tests required that no cyclic conditioning be conducted because of limited endurance of the mounting adhesive with some liner materials. The coefficient of friction test was based on dynamic coefficient of friction, as it proved to be a more reliable measurement than static coefficient of friction. The volumetric elasticity test required that air be released beneath samples in the test chamber before testing. The thermal conductivity test best reflected the clinical environment when thermal grease was omitted and when liner samples were placed under pressure consistent with load bearing conditions. The developed procedures provide a standardized approach for evaluating liner products in the prosthetics industry. Test results can be used to improve clinical selection of liners for individual patients and guide development of new liner products.
On the calculation of effective electric field in In0.53Ga0.47As surface channel metal-oxide-semiconductor field-effect-transistors (2011)

The effective electron mobility of In0.53Ga0.47As metal-oxide-semiconductor field-effect-transistors with HfO2 gate oxide was measured over a wide range of channel doping concentration. The back bias dependence of effective electron mobility was used to correctly calculate the vertical effective electric field. The effective electron mobility at moderate to high vertical effective electric field shows universal behavior independent of substrate impurity concentration.
EC626 Selection and Care of Dairy Sires -- The Nebraska Safety Bull Pen (1932)

Extension circular 626 provides information on the selection and care of dairy sires as well as the arrangement and advantages of the Nebraska safety bull pen.
Stimuli-responsive microwells for formation and retrieval of cell aggregates (2010)

Generating cell aggregates is beneficial for various applications ranging from biotechnology to regenerative therapies. Previously, poly(ethylene glycol) (PEG) microwells have been demonstrated as a potentially useful method for generating controlled-size cell aggregates. In addition to controlling cell aggregate size and homogeneity, the ability to confine cell aggregates on glass adhesive substrates and subsequently retrieve aggregates from microwells for further experimentation and analysis could be beneficial for various applications. However, it is often difficult to retrieve cell aggregates from these microwells without the use of digestive enzymes. This study describes the stable formation of cell aggregates in responsive microwells with adhesive substrates and their further retrieval in a temperature dependent manner by exploiting the stimuli responsiveness of these microwells. The responsive polymer structure of the arrays can be used to thermally regulate the microwell diameters causing a mechanical force on the aggregates, subsequently facilitating the retrieval of cell aggregates from the microwells with high efficiency compared to PEG arrays. This approach can be potentially integrated into high-throughput systems and may become a versatile tool for various applications that require aggregate formation and experimentation, such as tissue engineering, drug discovery, and stem cell biology.
Glycopeptide-preferring Polypeptide GalNAc Transferase 10 (ppGalNAc T10), Involved in Mucin-type O-Glycosylation, Has a Unique GalNAc-O-Ser/Thr-binding Site in Its Catalytic Domain Not Found in ppGalNAc T1 or T2 (2009)

Mucin-type O-gly co sy la tion is initiated by a large family of UDP-GalNAc:polypeptide alpha-N-acetylgalactosaminyltransferases (ppGalNAc Ts) that transfer GalNAc from UDP-GalNAc to the Ser and Thr residues of polypeptide acceptors. Some members of the family prefer previously gly co sylated peptides (ppGalNAc T7 and T10), whereas others are inhibited by neighboring gly co sy la tion (ppGalNAc T1 and T2). Characterizing their peptide and glycopeptide substrate specificity is critical for understanding the biological role and significance of each isoform. Utilizing a series of random peptide and glycopeptide substrates, we have obtained the peptide and glycopeptide specificities of ppGalNAc T10 for comparison with ppGalNAc T1 and T2. For the glycopeptide substrates, ppGalNAc T10 exhibited a single large preference for Ser/Thr-O-GalNAc at the +1 (C-terminal) position relative to the Ser or Thr acceptor site. ppGalNAc T1 and T2 revealed no significant enhancements suggesting Ser/Thr-O-GalNAc was inhibitory at most positions for these isoforms. Against random peptide substrates, ppGalNAc T10 revealed no significant hydrophobic or hydrophilic residue enhancements, in contrast to what has been reported previously for ppGalNAc T1 and T2. Our results reveal that these transferases have unique peptide and glycopeptide preferences demonstrating their substrate diversity and their likely roles ranging from initiating transferases to filling-in transferases.
Nuclear magnetic resonance studies of the solution chemistry of metal complexes. 22. Complexation of zinc by the growth-modulating tripeptide glycylhistidyllysine (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNuclear magnetic resonance studies of the solution chemistry of metal complexes. 22. Complexation of zinc by the growth-modulating tripeptide glycylhistidyllysineSusan A. Daignault, Alan P. Arnold, Anvarhusein A. Isab, and Dallas L. RabensteinCite this: Inorg. Chem. 1985, 24, 24, 3984–3988Publication Date (Print):November 1, 1985Publication History Published online1 May 2002Published inissue 1 November 1985https://pubs.acs.org/doi/10.1021/ic00218a005https://doi.org/10.1021/ic00218a005research-articleACS PublicationsRequest reuse permissionsArticle Views65Altmetric-Citations22LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Light load efficiency improvement of solar farms three-phase two-stage module integrated converter (2016)

In this paper, a method of maintaining high power conversion efficiency across the entire load range of a three-phase micro-inverter is proposed. The proposed control method substantially increases the conversion efficiency at light loads by minimizing switching losses of semiconductor devices as well as core losses of magnetic components. This is accomplished by entering a phase skipping operating mode wherein two phases of an inverter are disabled and three inverters combined to form a new three phase system with minimal grid imbalance. The performance of the proposed control method was evaluated on a 400W, 208VAC three-phase micro-inverter.
A Multidimensionality-Based DIF Analysis Paradigm (1996)

A multidimensionality-based differential item func tioning (DIF) analysis paradigm is presented that unifies the substantive and statistical DIF analysis approaches by linking both to a theoretically sound and mathematically rigorous multidimensional conceptualization of DIF. This paradigm has the potential (1) to improve under standing of the causes of DIF by formulating and testing substantive dimensionality-based DIF hypotheses; (2) to reduce Type 1 error through a better understanding of the possible multidimensionality of an appropriate matching criterion; and (3) to increase power through the testing of bundles of items measuring similar dimen sions. Using this approach, DIF analysis is shown to have the potential for greater integration in the overall test development process.
Convective instability of electrokinetic flows in a cross-shaped microchannel (2006)

We present a parametric experimental study of convective electrokinetic instability (EKI) in an isotropically etched, cross-shaped microchannel using quantitative epifluorescence imaging. The base state is a three-inlet, one-outlet electrokinetic focusing flow configuration where the centre sample stream and sheath flows have mismatched ionic conductivities. Electrokinetic flows with conductivity gradients become unstable when the electroviscous stretching and folding of conductivity interfaces grows faster than the dissipative effect of molecular diffusion. Scalar images, critical applied fields required for instability, and temporal and spatial scalar energy are presented for flows with a wide range of applied d.c. electric field and centre-to-sheath conductivity ratios. These parameters impose variations of the electric Rayleigh number across four orders of magnitude. We introduce a scaling for charge density in the bulk fluid as a function of local maximum conductivity gradients in the flow. This scaling shows that the flow becomes unstable at a critical electric Rayleigh number ( $Ra_{e, \ell} \,{=}\, 205$ ) and applies to a wide range of applied field and centre-to-sheath conductivity ratios. This work is relevant to on-chip electrokinetic flows with conductivity gradients such as field amplified sample stacking, flow at the intersections of multi-dimensional assays, electrokinetic control and separation of sample streams with poorly specified chemistry, and low-Reynolds number micromixing.
Amino Acid Substitutions Sufficient To Convert the Nontransforming p60 c- src Protein to a Transforming Protein (1986)

We have previously shown that Rous sarcoma virus variants that carry the cellular homolog (c-src) of the viral src gene (v-src) do not transform chicken embryo fibroblasts. We also have shown that replacement of sequences upstream or downstream from the BglI site of the cellular src gene with the corresponding regions of v-src restored transforming activity to the hybrid genes. Since there are only six amino acid changes between p60c-src and p60v-src within the sequences upstream from BglI, we constructed chimeric molecules involving v-src and c-src to determine the effect of each amino acid substitution on the biological activities of the gene product. We found that the change from Thr to Ile at position 338 or the replacement of a fragment of c-src containing Gly-63, Arg-95, and Thr-96 with a corresponding fragment of v-src containing Asp-63, Trp-95, and Ile-96 converted p60c-src into a transforming protein by the criteria of focus formation, anchorage-independent growth, and tumor formation in newborn chickens. These mutations also resulted in elevation of the protein kinase activity of p60c-src.
A 100μm diameter capacitive pressure sensor with 50 MPa dynamic range (2016)

This paper presents fully sealed absolute capacitive pressure sensors for high-pressure applications in hydraulic environments. The sensors have a o100 μm diaphragm and a nominal interelectrode gap of 3 μm. The interiors of the cavities are electrically isolated, allowing the sensors to operate at the high end of the pressure range with the center of the diaphragm in contact with the substrate beneath it. The sensors are monolithically fabricated using a combination of surface micromachining and through-wafer isolated bulk-silicon lead transfer for backside contacts. This structure allows the device footprints to be reduced to about 150 × 150 μm2, and simplifies system integration. Fabricated sensors with diaphragm thicknesses of 3 μm (C100t3) and 5 μm (C100t5) are tested in an oil environment at pressures up to 20 MPa and 50 MPa, respectively. The average sensitivities are 7200 ppm MPa−1 (3.1 fF MPa−1) for C100t3, and 3400 ppm MPa−1 (1.6 fF MPa−1) for C100t5 in the non-contact mode. In the contact mode, the average sensitivities are 9900 ppm MPa−1 (5.3 fF MPa−1) for C100t3, and 3100 ppm MPa−1 (1.6 fF MPa−1) for C100t5. A multiphysics finite element analysis approach that accommodates contact mode simulations is also presented.
Measurement of B+, B0 and Λb0 production in pPb collisions at sNN=8.16  TeV (2019)

The production of ${B}^{+}$, ${B}^{0}$ and ${\mathrm{\ensuremath{\Lambda}}}_{b}^{0}$ hadrons is studied in proton-lead collisions at a center-of-mass energy per nucleon pair of $\sqrt{{s}_{\mathrm{NN}}}=8.16\text{ }\text{ }\mathrm{TeV}$ recorded with the LHCb detector at the LHC. The measurement uses a dataset corresponding to an integrated luminosity of $12.2\ifmmode\pm\else\textpm\fi{}0.3\text{ }\text{ }{\mathrm{nb}}^{\ensuremath{-}1}$ for the case where the proton beam is projected into the LHCb detector (corresponding to measuring hadron production at positive rapidity) and $18.6\ifmmode\pm\else\textpm\fi{}0.5\text{ }\text{ }{\mathrm{nb}}^{\ensuremath{-}1}$ for the lead beam projected into the LHCb detector (corresponding to measuring hadron production at negative rapidity). Double-differential cross sections are measured and used to determine forward-backward ratios and nuclear modification factors, which directly probe nuclear effects in the production of beauty hadrons. The double-differential cross sections are measured as a function of the beauty-hadron transverse momentum and rapidity in the nucleon-nucleon center-of-mass frame. Forward-to-backward cross section ratios and nuclear modification factors indicate a significant nuclear suppression at positive rapidity. The ratio of ${\mathrm{\ensuremath{\Lambda}}}_{b}^{0}$ over ${B}^{0}$ production cross sections is reported and is consistent with the corresponding measurement in $pp$ collisions.
Tandem modeling investigations (2001)

An overview of work on Tandem acoustic modeling.
Co-targeting androgen receptor (AR) and DNA repair: A randomized ETS gene fusion-stratified trial of abiraterone + prednisone (Abi) +/- the PARP1 inhibitor veliparib for metastatic castration-resistant prostate cancer (mCRPC) patients (pts) (NCI9012)—A University of Chicago phase II consortium trial. (2016)

5010 Background: In preclinical CRPC models, PARP1 inhibition synergizes with AR targeted therapy and induces DNA damage, especially in ETS fusion-positive tumors. We hypothesized: co-targeting PARP-1 + AR is superior to AR inhibition and ETS is a response predictor. Methods: Eligible mCRPC pts underwent metastatic (mets) disease biopsy (bx), were stratified by ETS status and randomized to Abi (Arm A) or Abi + Veliparib (Arm B). The primary endpoint was confirmed PSA response rate (RR, ≥ 50% decline). Secondary endpoints included safety, objective RR (ORR), progression free survival (PFS), and if DNA repair gene deficiency (DRD; homozygous deletions or deleterious mutations: BRCA 1, BRCA 2, ATM, FANCA, PALB2, RAD51B, RAD51C) predicts response. 148 pts stratified by ETS status to be randomized to detect a 20% PSA RR improvement assuming a 5% 1-sided type I error and 80% power. Primary analysis: PSA RR logistic model to test the interaction of ETS status and treatment with a significance threshold of 0.15. Results: 185 eligible pts underwent mets bx (89 soft tissue, 96 bone); 159 (86 %) had adequate tissue, 35% were ETS +ve (IHC). 153 pts (88% white, 8% black, median age 68 years, median PSA 35.4 ng/ml) were randomized: Arms A/B: 74/79. Hyperglycemia was the most common G3/4 AE (≥ 5% in both arms). PSA RR: Arm A 63%, Arm B 67%. ORR: Arm A 36%, Arm B 49%. Median PFS: Arm A 8.5 months (m) (95% CI: 8.1 – 13.5), Arm B 11m (95% CI: 8.1 – 13.8). More Arm B pts were on therapy for 12 (34% vs 30%) and 18 + cycles (20% vs 16%). There were no differences by ETS status. Exploratory analysis from sequencing mets tissue (N=66) was performed. 18 pts (27%) had DRD; >80% in both arms had PSA response. Median PFS (95% Cl) by DRD status: no DRD: 5.8 ms (4.2 - 8.2) vs DRD 13.5 ms (8.2 - NR). Conclusions: This first mets biomarker-based trial was feasible. The trend is in favor of Abi + Veliparib with regard to PSA RR, ORR, therapy duration and mPFS. Exploratory analysis suggest that DRD is associated with better response. Updated clinical and sequencing data will be reported. Clinical trial information: NCT01576172.
Immune responses in a mouse model of vitiligo with spontaneous epidermal de‐ and repigmentation (2014)

Summary To generate a mouse model of spontaneous epidermal depigmentation, parental h3 TA 2 mice, expressing both a human‐derived, tyrosinase‐reactive T‐cell receptor on T cells and the matching HLA ‐A2 transgene, were crossed to keratin 14‐promoter driven, stem cell factor transgenic (K14‐ SCF ) mice with intra‐epidermal melanocytes. In resulting Vitesse mice, spontaneous skin depigmentation precedes symmetrical and sharply demarcated patches of graying hair. Whereas the SCF transgene alone dictates a greater retinoic acid receptor‐related orphan receptor gamma ( ROR γt) + T‐cell compartment, these cells displayed markedly increased IL ‐17 expression within Vitesse mice. Similar to patient skin, regulatory T cells were less abundant compared with K 14‐ SCF mice, with the exception of gradually appearing patches of repigmenting skin. The subtle repigmentation observed likely reflects resilient melanocytes that coexist with skin‐infiltrating, melanocyte‐reactive T cells. Similar repigmenting lesions were found in a different TCR transgenic model of vitiligo developed on an SCF transgenic background, supporting a role for SCF in repigmentation.
Analysis of Epidural Waveform to Determine Correct Epidural Catheter Placement After CSE Labor Analgesia (2021)

Pulsatile pressure waveform recording with CCDDS through the epidural catheter resulted in high sensitivity and positive predictive value which can help the proper placement of the epidural catheter.
The Electrochemistry of Silver Hollandite Nanorods, AgxMn8O16: Enhancement of Electrochemical Battery Performance via Dimensional and Compositional Control (2013)

The reflux synthetic approach for silver hollandite, AgxMn8O16, enabled the preparation of pure silver hollandite over a range of silver (x) content, 1.0 ≤ x ≤ 1.8. It was demonstrated that the crystallite size increased along with the silver content in the product. This finding led to the ability to synthetically control material properties enabling the systematic exploration of the electrochemistry of silver hollandite at a variety of crystallite sizes. Results from battery electrochemical testing, including constant current discharge, pulse testing, Galvanostatic Intermittent Titration Type Testing and cycle testing, are reported herein where all tests demonstrated improved performance of the smaller crystallite materials relative to the larger crystallite materials. Notably, a 5 fold decrease in calculated DC cell resistance and a doubling of the delivered capacity as a result of crystallite size reduction were demonstrated. Overall, these results illustrate an important paradigm in battery electrochemistry, that the electrochemistry of active electrode materials can be positively affected and controlled by variation in crystal size.
A Serine/Threonine Phosphorylation Site in the Ectodomain of a T Cell Receptor β Chain is Required for Activation by Superantigen (2003)

The presence of consensus phosphorylation sites in the ectodomains of cell surface proteins suggests that such post-translational modification may be important in regulation of surface receptor activity. To date, the only cell surface receptor for which such ectodomain phosphorylation has been conclusively demonstrated is the clonally expressed T cell antigen receptor (TCR). Attempts to conclusively identify individual phosphorylated residues in TCR alpha and beta chains and determine their functional significance by biochemical approaches failed due to insufficient quantities of purified molecules. Here we present the results of an alternative approach where survey of phosphorylation sites in the TCR alpha and beta chains was accomplished using site-directed mutagenesis and retroviral vector expression, as well as in vitro phosphorylation of synthetic peptide substrates. All mutants studied directed the cell surface expression of normal amounts of TCR, and all transfectants could be stimulated to produce IL-2 in response to substrate-immobilized antibody to TCR. However, mutation of serine-88 in the protein kinase A phosphorylation site of the TCR beta chain resulted in a complete lack of response to the superantigen staphylococcal enterotoxin B (SEB). In addition, this mutation abolished TCR-associated tyrosine phosphorylation, consistent with the impairment of cell signaling. Reversion of the serine-88/alanine mutation with phosphorylatable threonine completely restored the SEB recognition by TCR. These results, interpreted in the context of the known three-dimensional structure of the complex of SEB and TCR, are consistent with the view that serine-88 is important for the contact of the TCR beta chain with SEB.