gebhart/patrepeval huggingface dataset:
Hydrogen-deuterium exchange signature of porcine cerebroside sulfate activator protein (2000)

Hydrogen-deuterium exchange can be a sensitive indicator of protein structural integrity. Comparisons were made between cerebroside sulfate activator protein (CSAct) in the native state and after treatment with guanidine hydrochloride plus dithiothreitol. Native protein has three internal disulfide bonds and treated protein has no internal disulfide bonds. The comparisons were made using hydrogen-deuterium exchange measured by electrospray ionization mass spectrometry, percentage alpha-helical content measured by circular dichroism and biological activity measured by the ability to support arylsulfatase A-catalyzed sulfate hydrolysis from cerebroside sulfate. In acidic solvent native protein has 59 exchange refractory protons and treated protein has 20 exchange refractory protons (44 and 14% of the exchangeable proton populations, respectively). In native protein the size of the exchange refractory proton population is sensitive to changes in pH, temperature and the presence of a ligand. It is uninfluenced by the presence or absence of glycosyl groups attached to Asn21. Helical content is virtually identical in native and treated protein. Biological activity is significantly reduced but not obliterated in treated protein. The hydrogen-deuterium exchange profile appears to be a sensitive signature of the correctly folded protein, and reflects a dimension of the protein structure that is not apparent in circular dichroic spectra or in the ability of the protein to support arylsulfatase A-catalyzed sulfate hydrolysis from sulfatide. The hydrogen-deuterium exchange profile will be a valuable criterion for characterizing mutant forms of CSAct produced by recombinant and synthetic paradigms and also the native and mutant forms of related proteins.
Observation of charmless baryonic decays B(s)0→pp¯h+h′− (2017)

Decays of ${B}^{0}$ and ${B}_{s}^{0}$ mesons to the charmless baryonic final states $p\overline{p}{h}^{+}{h}^{\ensuremath{'}\ensuremath{-}}$, where $h$ and ${h}^{\ensuremath{'}}$ each denote a kaon or a pion, are searched for using the LHCb detector. The analysis is based on a sample of proton-proton collision data collected at center-of-mass energies of 7 and 8 TeV, corresponding to an integrated luminosity of $3\text{ }\text{ }{\mathrm{fb}}^{\ensuremath{-}1}$. Four-body charmless baryonic ${B}_{s}^{0}$ decays are observed for the first time. The decays ${B}_{s}^{0}\ensuremath{\rightarrow}p\overline{p}{K}^{+}{K}^{\ensuremath{-}}$, ${B}_{s}^{0}\ensuremath{\rightarrow}p\overline{p}{K}^{\ifmmode\pm\else\textpm\fi{}}{\ensuremath{\pi}}^{\ensuremath{\mp}}$, ${B}^{0}\ensuremath{\rightarrow}p\overline{p}{K}^{\ifmmode\pm\else\textpm\fi{}}{\ensuremath{\pi}}^{\ensuremath{\mp}}$ and ${B}^{0}\ensuremath{\rightarrow}p\overline{p}{\ensuremath{\pi}}^{+}{\ensuremath{\pi}}^{\ensuremath{-}}$ are observed with a significance greater than 5 standard deviations; evidence at 4.1 standard deviations is found for the ${B}^{0}\ensuremath{\rightarrow}p\overline{p}{K}^{+}{K}^{\ensuremath{-}}$ decay and an upper limit is set on the branching fraction for ${B}_{s}^{0}\ensuremath{\rightarrow}p\overline{p}{\ensuremath{\pi}}^{+}{\ensuremath{\pi}}^{\ensuremath{-}}$. Branching fractions in the kinematic region $m(p\overline{p})&lt;2850\text{ }\text{ }\mathrm{MeV}/{c}^{2}$ are measured relative to the ${B}^{0}\ensuremath{\rightarrow}J/\ensuremath{\psi}(\ensuremath{\rightarrow}p\overline{p}){K}^{*}(892{)}^{0}$ channel.
ChemInform Abstract: Magnetic Inducement of Texture in Ba2YCu3O6.9 Particle Assemblies Under Cryogenic Conditions. (1989)

Abstract Production of texture in powder and sintered microstructures of the title compound is achieved by applying a magnetic field while the specimen is in the superconducting state.
Amperometric Biosensors for Detection of Sugars Based on the Electrical Wiring of Different Pyranose Oxidases and Pyranose Dehydrogenases with Osmium Redox Polymer on Graphite Electrodes (2007)

Abstract Electrical wiring of different types of pyranose oxidase (P2O) (fungal wild type, recombinant wild type with a hexa‐histidine tag, mutant form E542K with a hexa‐histidine tag) from Trametes multicolor , and recombinant P2O from Coriolus sp. overexpressed in Escherichia coli as well as of pyranose dehydrogenase (PDH) from Agaricus meleagris and Agaricus xanthoderma with an osmium redox polymer (poly(1‐vinylimidazole) 12 ‐[Os(4,4′‐dimethyl‐2,2′‐dipyridyl) 2 Cl 2 ] 2+/+ ) on graphite electrodes was carried out. After optimization studies using glucose as substrate, the biosensors, which showed the best characteristics in terms of linear range, detection limit and sensitivity were selected, viz. wild type P2O from T. multicolor and PDH from A. meleagris. These two enzymes were used and investigated for their selectivity for a number of different sugars.
Aggressive repeat surgery for focally recurrent primary glioblastoma: outcomes and theoretical framework (2015)

OBJECT The relative benefit of repeat surgery for recurrent glioblastoma is unclear, in part due to the very heterogeneous nature of the patient population and the effect of clinician philosophy on the duration and aggressiveness of treatment. The authors sought to investigate the role of time to last recurrence on patient outcomes following aggressive repeat surgery for recurrent glioblastoma. METHODS The authors present outcomes in 104 patients undergoing repeat surgery for focally recurrent glioblastoma with at least 95% resection and adjuvant treatment at most recent prior surgery. In addition to common variables, they provide data regarding the period of progression-free survival (PFS) following an aggressive lesionectomy for focally recurrent primary glioblastoma (T2) and the time the tumor took to recur since the previous surgery (T1). They term the ratio T1/T2 the relative aggressivity index (RAI). RESULTS The median PFS was 7.8 months, 6.0 months, and 4.8 months following the second, third, and fourth-sixth craniotomies, respectively. Importantly, there was a wide range of outcomes, with time to postoperative recurrence ranging from 1 to 24 months in this group. Analysis showed no meaningful relationship between T1 and T2, meaning that previous PFS is entirely unable to predict the PFS that another surgery will provide the patient. CONCLUSIONS Repeat surgery for glioblastoma is beneficial in many cases, however this is hard to predict preoperatively. Often, surgery can provide the patient with a good period of disease freedom, but this is variable and in general it is not possible to reliably predict who these patients are.
Fluorescence spectroscopy of low-level endogenous β-adrenergic receptor expression at the plasma membrane of differentiating human iPSC-derived cardiomyocytes (2022)

Abstract The potential of human induced pluripotent stem cells (hiPSCs) to be differentiated into cardiomyocytes (CMs) mimicking the adult CMs functional morphology, marker genes and signaling characteristics has been investigated since over a decade. The evolution of the membrane localization of CM-specific G protein-coupled receptors throughout differentiation has received, however, only limited attention to date. We employ here advanced fluorescent spectroscopy, namely linescan Fluorescence Correlation Spectroscopy (FCS), to observe how the plasma membrane abundance of the β 1 - and β 2 -adrenergic receptors (β 1/2 -ARs), labelled using a bright and photostable fluorescent antagonist, evolves during long-term monolayer culture of hiPSC-derived CMs. We compare it to the kinetic of observed mRNA levels in wildtype (WT) hiPSCs and in two CRISPR/Cas9 knock-in clones. We conduct these observations against the backdrop of our recent report that β 2 -ARs, as opposed to β 1 -ARs, specifically segregate to the T-Tubular system of adult CMs.
Novel retinoic acid generating activities in the neural tube and heart identified by conditional rescue ofRaldh2null mutant mice (2002)

Retinoid control of vertebrate development depends upon tissue-specific metabolism of retinol to retinoic acid (RA). The RA biosynthetic enzyme RALDH2 catalyzes much, but not all, RA production in mouse embryos, as revealed here with Raldh2 null mutants carrying an RA-responsive transgene. Targeted disruption of Raldh2 arrests development at midgestation and eliminates all RA synthesis except that associated with Raldh3 expression in the surface ectoderm of the eye field. Conditional rescue of Raldh2(-/-) embryos by limited maternal RA administration allows development to proceed and results in the establishment of additional sites of RA synthesis linked to Raldh1 expression in the dorsal retina and to Raldh3 expression in the ventral retina, olfactory pit and urinary tract. Unexpectedly, conditionally rescued Raldh2(-/-) embryos also possess novel sites of RA synthesis in the neural tube and heart that do not correspond to expression of Raldh1-3. RA synthesis in the mutant neural tube was localized in the spinal cord, posterior hindbrain and portions of the midbrain and forebrain, whereas activity in the mutant heart was localized in the conotruncus and sinus venosa. In the posterior hindbrain, this novel RA-generating activity was expressed during establishment of rhombomeric boundaries. In the spinal cord, the novel activity was localized in the floorplate plus in the intermediate region where retinoid-dependent interneurons develop. These novel RA-generating activities in the neural tube and heart fill gaps in our knowledge of how RA is generated spatiotemporally and may, along with Raldh1 and Raldh3, contribute to rescue of Raldh2(-/-) embryos by producing RA locally.
In Vivo Remodeling and Structural Characterization of Fibrin-Based Tissue-Engineered Heart Valves in the Adult Sheep Model (2009)

Autologous fibrin-based tissue-engineered heart valves have demonstrated excellent potential as patient-derived valve replacements. The present pilot study aims to evaluate the structure and mechanical durability of fibrin-based heart valves after implantation in a large-animal model (sheep). Tissue-engineered heart valves were molded using a fibrin scaffold and autologous arterial-derived cells before 28 days of mechanical conditioning. Conditioned valves were subsequently implanted in the pulmonary trunk of the same animals from which the cells were harvested. After 3 months in vivo, explanted valve conduits (n = 4) had remained intact and exhibited native tissue consistency, although leaflets demonstrated insufficiency because of tissue contraction. Routine histology showed remarkable tissue development and cell distribution, along with functional blood vessel ingrowth. A confluent monolayer of endothelial cells was present on the valve surface, as evidenced by scanning electron microscopy and positive von Willebrand factor staining. Immunohistochemistry and extracellular matrix (ECM) assay demonstrated complete resorption of the fibrin scaffold and replacement with ECM proteins. Transmission electron microscopy revealed mature collagen formation and viable, active resident tissue cells. The preliminary findings of implanted fibrin-based tissue-engineered heart valves are encouraging, with excellent tissue remodeling and structural durability after 3 months in vivo. The results from this pilot study highlight the potential for construction of completely "autologous" customized tissue-engineered heart valves based on a patient-derived fibrin scaffold.
A Multiphase AC–DC Converter With Improved Power Quality for EV Charging Station (2021)

This work presents a multiphase ac&#x2013;dc converter-based electric vehicle (EV) charging station that is designed to meet IEEE-519 power-quality (PQ) standard. The proposed EV charging station draws current from the utility grid at low total harmonic distortion (THD) and high power factor (PF), without using PF corrector and active/passive filter circuits. This study evaluates an 18-phase ac&#x2013;dc converter for an EV charging station, which consists of three single-phase multiwinding transformers (MWTs) and diode rectifiers. MWTs are connected to enable direct conversion of a three-phase utility supply into an 18-phase power supply that reduces the measured THD in the input line current. The ac&#x2013;dc conversion stage of the proposed EV charging station is found to be reliable and operates at line-frequency, without the need for high-frequency active switches. For charging an EV in constant current (CC) and constant voltage (CV) mode, a multiphase (interleaved) dc&#x2013;dc converter is used. MATLAB/Simulink environment is used to evaluate the performance of the proposed EV charging station in terms of THD and PF. The results are validated through an on-the-bench prototype hardware setup of the proposed EV charging station.
Measurements of the electrohydrodynamic instability in planar geometry using gallium (1995)

The electrohydrodynamic (EHD) instability in planar geometry was observed using gallium as the working fluid. Electric fields in the range of 0.12–0.26 MV/cm were applied in pulse durations up to 14.4 ms. The instability of the gallium surface was observed using a box camera and pulsed backlighting. The measured wavelengths of the instability ranged from 1.2 to 17.0 mm, consistent with theoretical predictions provided a field enhancement factor of 1.5 is assumed on the meniscus of the gallium. A transverse magnetic field of about 0.6 T was found to completely dampen the instability along the magnetic field at an applied electric field of 0.2 MV/cm; the transverse direction was unaffected. This result puts limits on the usefulness of an EHD ion source in magnetically insulated diodes for inertial confinement fusion studies.
Proposed Report on Field Training of Public Health Personnel. Committee on Professional Education (1955)

Proposed Report on Field Training of Public Health Personnel. Committee on Professional Education Leroy E. Burney, Reginald M. Atwater, Philip E. Blackerby, Jr., Herbert M. Bosch, Robert D. Defries, Albert V. Hardy, S. S. Lifson, Otto T. Mallery, Jr., Edward G. McGavran, Janice E. Mickey, Dean W. Roberts, Dean F. Smiley, Robert G. Webster, Edward G. McGavran, William C. Gibson, M. R. Kinde, S. Elizabeth Lovell, Pearl McIver, James C. Malcolm, Hugh B. Robins, and Wilson G. Smillie CopyRight https://doi.org/10.2105/AJPH.45.10.1351 Published Online: August 29, 2011
Atomic Structure of the Immunophilin FKBP13-FK506 Complex: insights into the Composite Binding Surface for Calcineurin (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAtomic Structure of the Immunophilin FKBP13-FK506 Complex: insights into the Composite Binding Surface for CalcineurinL. Wayne Schultz, Patrick K. Martin, Jun Liang, Stuart L. Schreiber, and Jon ClardyCite this: J. Am. Chem. Soc. 1994, 116, 7, 3129–3130Publication Date (Print):April 1, 1994Publication History Published online1 May 2002Published inissue 1 April 1994https://pubs.acs.org/doi/10.1021/ja00086a055https://doi.org/10.1021/ja00086a055research-articleACS PublicationsRequest reuse permissionsArticle Views178Altmetric-Citations26LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Surgical Palliation Strategy Does Not Affect Interstage Ventricular Dysfunction or Atrioventricular Valve Regurgitation in Children With Hypoplastic Left Heart Syndrome and Variants (2013)

Surgical palliation strategy does not affect mortality, interstage ventricular function, or interstage AVVR in children with hypoplastic left heart syndrome. Therefore, the different timing and mechanisms of myocardial injury among palliation strategies do not affect outcomes. Ventricular dysfunction adversely affects transplant-free survival and atrioventricular valve function. Adverse events are associated with the development of ventricular dysfunction. To improve outcomes, interstage treatment should focus on the preservation of ventricular function.
In Vivo Silicon-Based Flexible Radio Frequency Integrated Circuits Monolithically Encapsulated with Biocompatible Liquid Crystal Polymers (2013)

Biointegrated electronics have been investigated for various healthcare applications which can introduce biomedical systems into the human body. Silicon-based semiconductors perform significant roles of nerve stimulation, signal analysis, and wireless communication in implantable electronics. However, the current large-scale integration (LSI) chips have limitations in in vivo devices due to their rigid and bulky properties. This paper describes in vivo ultrathin silicon-based liquid crystal polymer (LCP) monolithically encapsulated flexible radio frequency integrated circuits (RFICs) for medical wireless communication. The mechanical stability of the LCP encapsulation is supported by finite element analysis simulation. In vivo electrical reliability and bioaffinity of the LCP monoencapsulated RFIC devices are confirmed in rats. In vitro accelerated soak tests are performed with Arrhenius method to estimate the lifetime of LCP monoencapsulated RFICs in a live body. The work could provide an approach to flexible LSI in biointegrated electronics such as an artificial retina and wireless body sensor networks.
Thermal electron attachment to carbon suboxide (1989)

Thermal electron attachment to C3O2 has been studied by the pulse radiolysis–microwave cavity technique. The time variation of electron concentration for mixtures C3O2 with Ar and N2 shows decays with two components and the profile changes significantly depending on both C3O2 and buffer-gas pressures. This can be interpreted by a fast electron attachment to C3O2 to form a compound negative ion in equilibrium with the free electron followed by a slow stabilization of the ion leading to a stable ion. Simulations for the observed decay profiles indicate the presence of two electron attachment–detachment equilibria, one associated with electron and C3O2 and the other including electron, C3O2, and the buffer-gas molecule. The slow stabilization occurs by two-step three-body processes involving intermediate dimer ion. This is supported by a ‘‘negative temperature dependence’’ observed for the stabilization rates between 270 and 325 K. Though the electron affinity of C3O2 is negative, that of (C3O2)2 is likely to be positive. The role of electron attachment has been discussed in relation to an anomalously large cross section for ionization of a high-Rydberg atom by C3O2 reported previously.
Processing Amyloid Precursor Protein at the β-Site Requires Proper Orientation to Be Accessed by BACE1 (2004)

Membrane-bound BACE1 naturally cleaves its transmembrane substrate amyloid precursor protein (APP) at the two adjacent beta- and beta'-sites. Cleavage at these two sites generates the heterogeneous N-terminal end of APP C-terminal fragments that are further processed by gamma-secretase to release Abeta-(1-40/42) or Abeta-(11-40/42). The significance underlying Abeta-(11-40/42) in Alzheimer's disease pathogenesis has remained to be experimentally elucidated, but increased production of Abeta-(1-40/42) has been broadly demonstrated to contribute to amyloid depositions in senile plaques. In this study, we show that the cleavage of APP at the beta-site by BACE1 is readily disrupted through limited structural twists, whereas the beta'-site is relatively better positioned to gain access to the BACE1 catalytic cavity. Radical insertion or deletion of residues between beta- and beta'-site also favors cleavage of APP at the beta'-site. On the other hand, either lengthening or shortening the loop region of BACE1 has a minor impact on the selective cleavage of APP at these two adjacent sites, but significantly shortening the loop region impairs the ability of BACE1 to process APP at both sites. Thus, processing of APP by BACE1 is clearly dependent on a mutual structural compatibility in addition to the sequence feature. The knowledge gained from this study will potentially offer an opportunity for rational design of small molecule drugs to block the cleavage of APP specifically at the beta-site while not disturbing the functions of other cellular aspartyl proteases.
PHA bioplastic: A value‐added coproduct for biomass biorefineries (2009)

Abstract The petroleum industry has optimized profits by producing value‐added coproducts, such as plastics and chemicals, in addition to primary liquid fuels. A similar coproduct strategy applied to biorefineries processing cellulosic biomass to liquid fuels and/or energy would transform a technology that is marginally economic, depending on oil prices, to a sustainable business with enhanced revenue streams from multiple coproducts. The challenge is finding a biobased coproduct that is compatible with a biorefinery scenario and where markets warrant its production on a similar scale as liquid fuels and/or energy. Polyhydroxyalkanoate (PHA) bioplastics represent a coproduct that would be entirely compatible with either production of liquid fuels by hydrolyzing the residual biomass after PHA extraction or by alternative thermochemical processes. PHA bioplastics possess properties making them suitable replacements for many of the applications currently served by petroleum‐based plastics, thus providing tremendous market potential. Proof‐of‐concept technology for production of these plastics in several crops of agronomic interest has been demonstrated. In this review, we show that the potential for developing biomass‐based biorefineries producing liquid fuels and a value‐added coproduct is both real and realizable. Examples using switchgrass producing PHA bioplastics as a coproduct are described. © 2009 Society of Chemical Industry and John Wiley &amp; Sons, Ltd.
Dispersion-shifted segmented-core single-mode fibers (1984)

Dispersion-shifted segmented-core single-mode designs with low sensitivity of the zero-dispersion wavelength to the cutoff-wavelength variation have been identified. Dispersion, spot size, and bending-loss results on such dispersion-shifted fibers with the highest cutoff wavelength reported to date are presented.
Targeted inhibition of Snail family zinc finger transcription factors by oligonucleotide-Co(III) Schiff base conjugate (2009)

A transition metal complex targeted for the inhibition of a subset of zinc finger transcription factors has been synthesized and tested in Xenopus laevis. A Co(III) Schiff base complex modified with a 17-bp DNA sequence is designed to selectively inhibit Snail family transcription factors. The oligonucleotide-conjugated Co(III) complex prevents Slug, Snail, and Sip1 from binding their DNA targets whereas other transcription factors are still able to interact with their target DNA. The attachment of the oligonucleotide to the Co(III) complex increases specificity 150-fold over the unconjugated complex. Studies demonstrate that neither the oligo, or the Co(III) Schiff base complex alone, are sufficient for inactivation of Slug at concentrations that the conjugated complex mediates inhibition. Slug, Snail, and Sip1 have been implicated in the regulation of epithelial-to-mesenchymal transition in development and cancer. A complex targeted to inactivate their transcriptional activity could prove valuable as an experimental tool and a cancer therapeutic.