gebhart/patrepeval huggingface dataset:
Humoral immune functions in IL-4 transgenic mice (1991)

We have analyzed mice expressing IL-4 as a transgene, and found that expression of this lymphokine has profound effects on B cell function. B cells from transgenic mice exhibit phenotypic changes, including an increase in size and elevated expression of class II MHC. IL-4 increases the quantity of IgE produced by transgenic-derived B cells in response to LPS stimulation. In vivo, IL-4 markedly affects the serum Ig isotype repertoire. Serum levels of IgG1 and IgE are elevated, and levels of IgG2a, IgG2b, and IgG3 are depressed in IL-4 transgenic mice. Ag-specific antibody responses to immunization with hapten-carrier conjugates are also affected by IL-4. Transgenic mice show increased anti-hapten IgE and IgG1 and reduced anti-hapten IgG2a, IgG2b, and IgG3, compared with wild-type mice. Ag-specific IgE is substantially induced by T cell-dependent Ag, but not T cell-independent Ag, suggesting that cognate T-B interactions in addition to IL-4 are required for generating IgE responses in vivo. In vivo treatment with the anti-IL-4 mAb 11B11 reverses many of the isotype alterations in the transgenic mice, indicating that these changes arise as a direct consequence of IL-4 secretion.
Distinct fates of monocytes and T cells directly activated byPseudomonas aeruginosaexoenzyme S (2002)

Gram-negative infections can cause overwhelming inflammatory responses. Although factors other than LPS are clearly involved, these factors and their mechanisms of action have been poorly defined. During studies of LPS-independent inflammatory responses of the gram-negative pathogen Pseudomonas aeruginosa, an important virulence factor (exoenzyme S) was shown to be a potent mitogen for T cells. The current work demonstrates that exoenzyme S selectively induced transcription and secretion of biologically active cytokines and chemokines (chemotactic for neutrophils and T cells) from monocytes. Exoenzyme S stimulated highly purified monocytes independent of T cells. In addition, exoenzyme S stimulated T cells directly; neither T-cell activation (CD69) nor apoptosis (hypodiploidy) required the presence of monocytes. However, T-cell activation was enhanced via a noncontact-dependent mechanism as a result of the secretion of TNF-alpha and IL-6. This study identifies a unique property of a gram-negative-derived microbial product capable of activating multiple cell types and suggests a mechanism by which exoenzyme S contributes to the immunopathogenesis of cystic fibrosis and sepsis in patients infected with P. aeruginosa.
Theranostic Combination of Molecularly Targeted Radiotherapy and Immunotherapy Optimizes Therapeutic Response in a Syngeneic Murine Model of Melanoma (2018)

178 Objectives: In situ vaccination is achievable in GD2+ (disialoganglioside-2) tumor-bearing mice by using a local combination of 12Gy external beam radiation (RT) followed by intratumoral (IT) immunocytokine (IC) [hu14.18-IL2, an anti-GD2 antibody fused to IL2]. It is hypothesized that combining molecularly targeted radiotherapy (MTRT) with RT+IT-IC could improve in situ vaccination. The goals of our study were to determine the administered activity of MTRT that maximizes the therapeutic response of the combination with RT+IT-IC and estimate the corresponding absorbed dose to the tumor. To achieve these goals, our tumor-targeting alkylphosphocholine (NM600) was radiolabeled with the theranostic pair 86Y/90Y. Methods: C57BL/6 female mice bearing a syngeneic B78 melanoma flank tumor were administered 9.25 MBq of 86Y-NM600 (n=3) IV, and longitudinal microPET/CT scans were acquired at 2, 16, 24, and 48 h post injection (p.i.). CT-based contours of the tumor and organs of interest were overlaid on the PET volumes; tumor selectivity and biodistribution were reported as %ID/g. Using 86Y-NM600 PET image volumes and a Monte Carlo based dosimetry platform, the mean Gy/MBq delivered to B78 tumors and normal tissues by the therapeutic analog 90Y-NM600 was estimated. For the treatment studies, groups of mice bearing B78 flank tumors (200-300 mm3; n=5) were administered a single IV dose of 90Y-NM600 (0.925, 1.85, or 3.7 MBq) one week prior to RT+IT-IC and tumor growth was monitored via caliper measurements. Results: MicroPET/CT imaging confirmed selectivity and retention of 86Y-NM600 in B78 tumors (3 %ID/g at 48 h) and hepatobiliary excretion of the tracer (6 %ID/g at 48 h in the liver). Mouse-specific dosimetry estimates show the tumor dose (1.5 Gy/MBq) was higher than all contoured organs except for the liver (2.7 Gy/MBq). Preliminary results suggest that B78 tumor-bearing mice treated with 90Y-NM600 combined with RT+IT-IC may elicit enhanced tumor regression compared to RT+IT-IC alone. A dose titration of 90Y-NM600 demonstrated that 1.85 MBq, which corresponded to an absorbed dose of approximately 3 Gy, had the largest increase in therapeutic response when combined with RT+IT-IC. However, both 0.925 MBq (1.5 Gy tumor dose) and 3.7 MBq (6 Gy tumor dose) exhibited similar degrees of tumor regression, which may suggest that the major anti-tumor mechanism is the immune response, while the RT and 90Y-NM600 are making the tumor microenvironment more responsive to immunotherapy. Conclusions: Our preliminary results suggest that the synergy between MTRT and RT+IT-IC for the treatment of murine melanoma may be optimized by using theranostic agents and patient-specific prescriptions of MTRT to avoid abrogation of a synergistic therapeutic impact on the anti-tumor immune response.
Heteroconjugate antibody-directed killing of autologous human renal carcinoma cells by in vitro-activated lymphocytes. (1990)

Tumor cell lysis can be enhanced significantly in vitro when heteroconjugate (HC) antibodies (anti-CD3 x anti-tumor mAb) are used to specifically direct lymphocyte effector cells to the tumor cell target. In order to effectively utilize HC antibodies in an immunotherapy protocol, methods must be identified for the optimum expansion, activation, and retargeting of lymphocyte-effector populations from cancer patients. In this study, we have compared the proliferative responses of different normal and renal cell carcinoma (RCC) patient lymphocyte preparations (PBL, tumor-infiltrating lymphocytes) stimulated in vitro for periods up to 12 days with a variety of growth factor combinations (anti-CD3, rIL-2, rIL-4). These activated lymphocyte preparations were then tested in vitro for their ability to kill RCC tumor cells and tumor cell lines in the presence of HC preparations (anti-CD3 mAb covalently linked to mAb reactive to different RCC tumor-associated Ag). RCC patient PBL cultured with anti-CD3 plus rIL-2 for 12 days resulted in a 3- to 160-fold expansion of effector cells. These cells, as well as tumor infiltrating lymphocytes, when retargeted with appropriate HC antibodies were capable of mediating high levels of killing of autologous tumor cells. No constitutive autologous anti-tumor cell response was detected in the absence of added HC antibodies. Of the five anti-RCC mAb tested (A6H, K29, K20, UR07, and URO 3), HC containing URO 3 x anti-CD3 and K20 x anti-CD3 elicited the highest level of tumor cell lysis by the activated lymphocyte effector cells. Together these results demonstrate that HC antibodies may be a useful imunotherapeutic reagent for directing the killing of RCC tumor cells by autologous lymphocytes.
Prepregnancy body mass index and dietary intake in the first trimester of pregnancy (2006)

This study identified that women with a high prepregnancy BMI are more likely to under-report nutrient intakes. The finding that folate intake was significantly lower in heavier women accurately reporting dietary intake is of particular concern.
RNA:DNA hybrids in the human genome have distinctive nucleotide characteristics, chromatin composition, and transcriptional relationships (2015)

Overall, there is little to indicate a dependence for RNA:DNA hybrids forming co-transcriptionally, with results from the ribosomal DNA repeat unit instead supporting the intriguing model of RNA generating these structures in trans. The results of the study indicate heterogeneous functions of these genomic elements and new insights into their formation and stability in vivo.
Bio‐Inspired Synthesis of High‐Performance Nanocomposite Catalysts for Hydrogen Oxidation (2013)

Abstract A biologically inspired synthesis method is presented as a new tool for the design of novel electrochemically active materials, focusing on the advantages for fuel cell development. The need for cost‐effective, high‐performance materials is driving contemporary fuel cell research, with the expectation that advances in synthetic methods will be necessary for commercialization of this energy technology. Highly active electrocatalysts for proton‐exchange‐membrane (PEM) fuel cells are being developed, by combining a kinetically controlled synthesis method of the nanocrystalline metal catalyst with the mesoscale assembly of two morphologically different carbon building blocks of the supporting matrix. These methods provide access to new combinations of porosity, conductivity and electrochemical hydrogen oxidation. The relationships between the porous morphologies of the carbon matrices, the sizes of the platinum nanocrystals and their resulting electrochemical activities are discussed, correlating these with the relevant fuel cell principles.
Determining attenuation and propagation constants of microstrip line in long-wave infrared (2018)

Low-loss planar transmission lines are required for integrated optical or plasmonic nanocircuits. Full characterization of these lines is necessary for designing nanocircuits. This paper shows a method to calculate the attenuation and propagation constants of a patch-antenna-coupled microstrip transmission line (MTL) at 28.3THz that is suitable for measurement implementation via near-field microscopy techniques. After illumination with a Gaussian beam, a standing wave is formed by the electric near field along the MTL observed at the metal-air interface. By fitting an analytical standing wave expression to the near-field standing wave, the attenuation and propagation constants are determined. With the MTL characterized, a similar technique can be applied to determine the input impedance of an unknown load fed by the MTL. The quantification of antenna impedance and transmission line parameters provide requisite information for improving impedance matching and collection efficiency. Ansys High Frequency Structure Simulator (HFSS) is implemented to predict the computational results.
Discovery of 3,5-Diamino-1,2,4-triazole Ureas as Potent Anaplastic Lymphoma Kinase Inhibitors (2011)

A series of novel 3,5-diamino-1,2,4-triazole benzyl ureas was identified as having potent anaplastic lymphoma kinase (ALK) inhibition exemplified by 15a, 20a, and 23a, which exhibited antiproliferative IC(50) values of 70, 40, and 20 nM in Tel-ALK transformed Ba/F3 cells, respectively. Moreover, 15a and 23a potently inhibited the growth and survival of NPM-ALK positive anaplastic large cell lymphoma cell (SU-DHL-1) and neuroblastoma cell lines (KELLY, SH-SY5Y) containing the F1174L ALK mutation. These compounds provide novel leads for the development of small-molecule ALK inhibitors for cancer therapy.
Insulin receptor monoclonal antibodies that mimic insulin action without activating tyrosine kinase (1989)

HTC rat hepatoma cells were transfected with human insulin receptor cDNA to a level of 40,000 receptors/cell. In these cells, as well as in nontransfected cells, insulin stimulated the uptake of alpha-aminoisobutyric acid. Two monoclonal antibodies directed against the human insulin receptor alpha subunit, like insulin, stimulated amino acid uptake in transfected HTC cells, but not in nontransfected HTC cells. The antibodies, in contrast to insulin, failed to stimulate insulin receptor tyrosine kinase activity, both in intact transfected cells and in cell free extracts prepared from them. These data suggest, therefore, that activation of insulin receptor tyrosine kinase may not be an obligatory step in all of the transmembrane signaling mechanisms of the insulin receptor.
Neo-Adjuvant Chemotherapy Reduces, and Surgery Increases Immunosuppression in First-Line Treatment for Ovarian Cancer (2021)

The implementation of immunotherapy in the primary treatment schedule of ovarian cancer cannot be induced blindly. Carboplatin-paclitaxel seems to ameliorate the hostile immune microenvironment in ovarian cancer, which is less pronounced at the end of primary treatment. This prospective study during primary therapy for ovarian cancer that also looks at the evolution of immune-related proteins provides us with an insight into the temporary windows of opportunity in which to introduce immunotherapy during primary treatment.
InP- and GaAs-Based Photonic Power Converters Under O-Band Laser Illumination: Performance Analysis and Comparison (2022)

Photonic power converters (PPCs), which convert narrow-band light to electricity, are essential components in power-by-light systems. When designed for telecommunications wavelengths such as the O-band, near 1310 nm, the devices are well-suited to power-over-fiber applications. Despite the potential for very high power conversion efficiencies ( <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex-math notation="LaTeX">$\mathbf {&gt;50\%}$</tex-math></inline-formula> ), PPCs can be adversely affected by high-intensity nonuniform illumination conditions. In this work, we characterized two O-band PPC designs based on: high-quality InGaAsP absorber material lattice-matched to an InP substrate, and metamorphic InGaAs absorber material lattice-mismatched to a GaAs substrate, a more cost-effective and scalable alternative. We measured each device under O-band laser illumination with five beam profiles having peak-to-average ratios ranging from 2 to 11. Both devices were insensitive to the beam uniformity for input illumination with average irradiance below 2 W/cm <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex-math notation="LaTeX">$\mathbf {^{2}}$</tex-math></inline-formula> over their 5.4-mm <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex-math notation="LaTeX">$\mathbf {^{2}}$</tex-math></inline-formula> active areas, but exhibited better open-circuit voltages under larger, more uniform illumination profiles at higher incident powers. Measured efficiencies reached 52.8% and 48.7% for the lattice-matched and mismatched devices, respectively. Distributed circuit modeling results suggested that both lateral conduction losses and localized heating effects were responsible for the measured dependence on beam-size. Our work demonstrates the potential for O-band PPCs, presenting two highly efficient designs suitable for powering devices requiring <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex-math notation="LaTeX">$\mathbf {\lesssim 250}$</tex-math></inline-formula> mW, with an appropriate illumination profile.
Chromatic Dispersion Compensation by Frequency Domain Based All-Pass Filtering (2008)

We propose an alternative, block-wise, frequency domain based all-pass filter for chromatic dispersion compensation in uncompensated transmission links with coherent detection. Due to its structure with low complexity, we believe, that implementation in FPGAs for 40Gbit/s CP-QPSK is possible.
A 59GHz-to-67GHz 65nm-CMOS High Efficiency Power Amplifier (2011)

International audience
ChemInform Abstract: The Kondrat′eva Reaction in Flow: Direct Access to Annulated Pyridines. (2013)

Abstract The direct synthesis of cycloalka[c]pyridines via the title reaction by using continuous flow reaction conditions is developed.
DEMONSTRATION OF DELAYED HYPERSENSITIVITY TO SOLUBLE ANTIGENS OF CHEMICALLY INDUCED TUMORS BY INHIBITION OF MACROPHAGE MIGRATION (1969)

Sensitization to soluble antigens of chemically induced tumors is demonstrable in syngeneic strain 13 (inbred) guinea pigs by specific inhibition of macrophage migration. According to their capacity to (a) inhibit the migration of peritoneal cells from sensitized strain 13 donors or (b) release migration-inhibitory factor from their lymph node cells, the soluble antigens of three different chemically induced tumors did not cross react. This is in keeping with the absence of cross-reactivity among the same three tumors in previous tests of transplantation resistance or delayed hypersensitivity in vivo. Thus, inhibition of macrophage migration may provide a feasible assay in vitro for use in defining these soluble antigens further by physicochemical methods.
STUB1 is an intracellular checkpoint for interferon gamma sensing (2020)

Abstract Immune checkpoint blockade (ICB) leads to durable and complete tumour regression in some patients but in others gives temporary, partial or no response. Accordingly, significant efforts are underway to identify tumour-intrinsic mechanisms underlying ICB resistance. Results from a published CRISPR screen in a mouse model suggested that targeting STUB1, an E3 ligase involved in protein homeostasis, may overcome ICB resistance but the molecular basis of this effect remains unclear. Herein, we report an under-appreciated role of STUB1 to dampen the interferon gamma (IFNγ) response. Genetic deletion of STUB1 increased IFNGR1 abundance on the cell surface and thus enhanced the downstream IFNγ response as showed by multiple approaches including Western blotting, flow cytometry, qPCR, phospho-STAT1 assay, immunopeptidomics, proteomics, and gene expression profiling. Human prostate and breast cancer cells with STUB1 deletion were also susceptible to cytokine-induced growth inhibition. Furthermore, blockade of STUB1 protein function recapitulated the STUB1 -null phenotypes. Despite these encouraging in vitro data and positive implications from clinical datasets, we did not observe in vivo benefits of inactivating Stub1 in mouse syngeneic tumour models – with or without combination with anti-PD-1 therapy. However, our findings elucidate STUB1 as a barrier to IFNγ sensing, prompting further investigations to assess if broader inactivation of human STUB1 in both tumors and immune cells could overcome ICB resistance.
Picosecond measurements of gain-switching in a semiconductor laser driven by ultrashort electrical pulses (1987)

We report on the temporal profile of optical pulses emitted by a semiconductor laser when driven by 17 ps electrical pulses. These optical pulses are detected with subpicosecond resolution by optical up-conversion sampling[1,2]. The short duration of our electrical pulses distinguishes this work from previously published reports on gain- switching experiments. The short electrical pulses enable us to operate in a regime where the optical pulse widths are limited only by the laser, even when it is biased at threshold. We have investigated how the risetime, falltime, FWHM, amplitude and delay time of the gain-switched pulses vary with the semiconductor laser’s operating conditions (e.g. dc bias current, the width and amplitude of the driving pulse, and temperature).
HDR-LFNet: Inverse Tone Mapping using Fusion Network (2022)

To capture the real-world luminance values, High Dynamic Range (HDR) image processing has been developed. HDR images have a richer content than the widely-used Standard Dynamic Range (SDR) images, and are used in a number of situations, e.g. in film industry. As HDR displays are more and more commercially available, we need to be able to process HDR images as well as SDR ones (for example, devising denoising algorithms, inpaiting or anti-aliasing). The most powerful methods to process images are now deep neural networks. However, the training of such networks calls for a lot of images, and HDR images datasets are relatively small. One way to generate HDR images is inverse tone mapping operators (iTMOs). They are algorithms that expand the dynamic range of SDR images. In this paper, we propose HDR-LFNet, a novel iTMO, and its HDR training dataset. Our method merges several existing handcrafted iTMOs, combined in a supervised neural network to produce an HDR output. Our lightweight network requires less training images than state-of-the-art methods, and has faster training phase. Besides, the quality of the generated images is similar to the state-of-the-art. We present the architecture as well as the subjective and experimental evaluations of our method.
Molecular design and synthetic approach to C2,C3,C4-modified carbazoles: high triplet energy bipolar host materials for efficient blue phosphorescent organic light emitting diodes (2019)

Herein, we report the first examples of the design of and synthetic approach to C3/C4 and C2/C3/C4-modified carbazole based high triplet energy bipolar host materials for blue phosphorescent organic light emitting diodes and demonstrated excellent device performance with the maximum external quantum efficiency as high as 25.3% and current efficiency of 48.1 cd A-1.
Preliminary studies in some aspects of amino acid biosynthesis in juveniles of Penaeus monodon Fabricius: I. Incorporation of 14C from (U-14C) acetate into amino acids to precipitable proteins. (1980)

SEAFDEC Contribution No. 95.
CHOROIDAL VASCULAR HYPERPERMEABILITY AS A PREDICTOR OF TREATMENT RESPONSE FOR POLYPOIDAL CHOROIDAL VASCULOPATHY (2017)

The presence of CVH is associated with better visual outcome in eyes with polypoidal choroidal vasculopathy and lower injection number in combination therapy. Thus, CVH, but not choroidal thickness, should be further evaluated as a potential biomarker for selecting patients for combination therapy.
Silver Nanoparticle Behavior, Uptake, and Toxicity in Caenorhabditis elegans: Effects of Natural Organic Matter (2014)

Significant progress has been made in understanding the toxicity of silver nanoparticles (Ag NPs) under carefully controlled laboratory conditions. Natural organic matter (NOM) is omnipresent in complex environmental systems, where it may alter the behavior of nanoparticles in these systems. We exposed the nematode Caenorhabditis elegans to Ag NP suspensions with or without one of two kinds of NOM, Suwannee River and Pony Lake fulvic acids (SRFA and PLFA, respectively). PLFA rescued toxicity more effectively than SRFA. Measurement of total tissue silver content indicated that PLFA reduced total organismal (including digestive tract) uptake of ionic silver, but not of citrate-coated Ag NPs (CIT-Ag NPs). The majority of the CIT-Ag NP uptake was in the digestive tract. Limited tissue uptake was detected by hyperspectral microscopy but not by transmission electron microscopy. Co-exposure to PLFA resulted in the formation of NOM-Ag NP composites (both in medium and in nematodes) and rescued AgNO3- and CIT-Ag NP-induced cellular damage, potentially by decreasing intracellular uptake of CIT-Ag NPs.
High quality factor etchless silicon photonic ring resonators (2011)

We demonstrate high quality factor etchless silicon photonic ring resonators fabricated by selective thermal oxidation of silicon without the silicon layer being exposed to any plasma etching throughout the fabrication process. We achieve a high intrinsic quality factor of 510,000 in 50 µm-radius ring resonators, corresponding to a ring loss of 0.8 dB/cm. The device has a total chip insertion loss of 2.5 dB, achieved by designing etchless silicon inverse nanotapers at both the input and output of the chip.
The Immunome in Two Inherited Forms of Pulmonary Fibrosis (2018)

www.ClinicalTrials.gov, identifiers NCT00968084, NCT01200823, NCT00001456, and NCT00084305.