gebhart/patrepeval huggingface dataset:
Effect of a quality-controlled fermented nutraceutical on skin aging markers: An antioxidant-control, double-blind study (2016)

The aim of the present study was to determine whether oral supplementation with a fermented papaya preparation (FPP-treated group) or an antioxidant cocktail (antioxidant-control group, composed of 10 mg trans-resveratrol, 60 µg selenium, 10 mg vitamin E and 50 mg vitamin C) was able to improve the skin antioxidant capacity and the expression of key skin genes, while promoting skin antiaging effects. The study enrolled 60 healthy non-smoker males and females aged 40-65 years, all of whom showed clinical signs of skin aging. The subjects were randomly divided into two matched groups, and were administered FPP or antioxidant treatment of a 4.5 g/day sachet sublingually twice a day for 90 days in a double-blind fashion. The parameters investigated were: Skin surface, brown spots, skin evenness, skin moisturization, elasticity (face), redox balance, nitric oxide (NO) concentration, and the expression levels of key genes (outer forearm sample). As compared with the baseline (day 0) and antioxidant-control values, FPP-treated subjects showed a significant improvement in skin evenness, moisturization and elasticity. The two treatments improved the MDA and SOD skin concentrations, but only the FPP-treated group showed a higher SOD level and a significant NO increase, along with significant upregulation of acquaporin-3 and downregulation of the potentially pro-aging/carcinogenetic cyclophilin-A and CD147 genes (P<0.05). Progerin was unaffected in both treatment groups. In conclusion, these findings suggest that orally-administered FPP showed a consistent biological and gene-regulatory improvement in the skin, as was also demonstrated in previous experimental and clinical trials testing other tissues, while common oral antioxidants had only a minor effect.
Co-purification of A1 Adenosine Receptors and Guanine Nucleotide-binding Proteins from Bovine Brain (1989)

A1 adenosine receptors and guanine nucleotide-binding proteins (G proteins) solubilized with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate have been co-purified from bovine cerebral cortex. A portion of solubilized receptors which displays high affinity GTP-sensitive agonist binding (40-50%) adheres tightly to agonist affinity columns composed of N6-aminobenzyladenosine-agarose. A1 adenosine receptors and G proteins are rapidly and selectively coeluted from agonist columns by the addition of 8-p-sulfophenyltheophylline, but only in combination with Mg2+-GTP or N-ethylmaleimide, agents which lower the affinity of receptors for agonists. Purified receptors and G protein alpha-subunits can be detected with the potent A1-selective antagonist radioligand, [125I]3-(4-amino-3-iodo)phenethyl-1-propyl-8-cyclopentylxanthine (125I-BW-A844U) and [35S]guanosine 5'-3-O-(thio)triphosphate [( 35S]GTP gamma S), respectively. Pretreatment of solubilized receptors with 0.1 mM N-ethylmaleimide or 0.1 mM R-phenylisopropyladenosine abolishes adsorption of receptors and G proteins to affinity columns. Following removal of 8-p-sulfophenyltheophylline and GTP, purified receptors bind agonists (2 sites) and antagonists (1 site) with affinities similar to crude soluble receptors and typical of A1 receptors. Some receptors may be denatured as a result of purification since only 23% of the radioligand binding sites which adhere to the affinity column can be detected in the eluate. The Bmax of purified receptors, 820 +/- 100 pmol/mg protein (n = 3) is 1800-fold higher than crude soluble receptors. The specific activity of [35S]GTP gamma S binding sites in affinity column eluates is 4640 pmol/mg protein. Assuming a 1:1 stoichiometry, this specific activity indicates that receptor-G protein complexes are greater than 50% pure following affinity chromatography. The photoaffinity labeled purified receptor was identified by polyacrylamide gel electrophoresis as a single band with a molecular mass of 35 kDa which when deglycosylated undergoes a characteristic shift in molecular mass to a sharp band at 32 kDa. In addition to the receptor, silver staining revealed polypeptides with molecular masses of 39 and 41 kDa, which are ADP-ribosylated by pertussis toxin, and 36 kDa corresponding to G protein beta-subunits.
Modulation of drought resistance by the abscisic acid receptor PYL5 through inhibition of clade A PP2Cs (2009)

Abscisic acid (ABA) is a key phytohormone involved in adaption to environmental stress and regulation of plant development. Clade A protein phosphatases type 2C (PP2Cs), such as HAB1, are key negative regulators of ABA signaling in Arabidopsis. To obtain further insight into regulation of HAB1 function by ABA, we have screened for HAB1-interacting partners using a yeast two-hybrid approach. Three proteins were identified, PYL5, PYL6 and PYL8, which belong to a 14-member subfamily of the Bet v1-like superfamily. HAB1-PYL5 interaction was confirmed using BiFC and co-immunoprecipitation assays. PYL5 over-expression led to a globally enhanced response to ABA, in contrast to the opposite phenotype reported for HAB1-over-expressing plants. F(2) plants that over-expressed both HAB1 and PYL5 showed an enhanced response to ABA, indicating that PYL5 antagonizes HAB1 function. PYL5 and other members of its protein family inhibited HAB1, ABI1 and ABI2 phosphatase activity in an ABA-dependent manner. Isothermal titration calorimetry revealed saturable binding of (+)ABA to PYL5, with K(d) values of 1.1 mum or 38 nm in the absence or presence of the PP2C catalytic core of HAB1, respectively. Our work indicates that PYL5 is a cytosolic and nuclear ABA receptor that activates ABA signaling through direct inhibition of clade A PP2Cs. Moreover, we show that enhanced resistance to drought can be obtained through PYL5-mediated inhibition of clade A PP2Cs.
Titanocene-Based Method for Indole Synthesis (1998)

A new approach for the construction of indoles employing the air- and moisture-stable reagent Cp2TiCl2 is described. The key steps involved are (1) the intermolecular insertion reactions of an olefin and a titanocene-stabilized benzyne complex and (2) the Pd-catalyzed aryl amination reaction. The simplicity and availability of the requisite starting materials give the method a broad scope for the preparation of polysubstituted indoles.
Optimizing Electrocardiograph Electrode Placement for Cardiac-Gated Magnetic Resonance Imaging (1987)

Electrocardiographs recorded in a magnetic field for cardiac-gating in magnetic resonance imaging (MRI) are complicated by blood flow-induced potentials. This study examines which lead of the standard 12-lead ECG maximizes the QRS while minimizing flow-induced interference. Twelve-lead ECGs were performed on normal volunteers (n = 9) and patients (n = 13) in and out of the bore of a 1.5 Tesla imaging magnet. The amplitude of the major flow-induced potentials was measured, and the vectors of largest induced potential and the QRS axis were plotted for each subject. ECGs obtained outside and inside the magnet were digitized and subtracted (in magnet ECG--out of magnet ECG = artifact ECG) and the peaks of the resultant curves measured. Superimposed potentials were largest in the early T wave and late S-T segment in leads I, II, V1, and V2, and smallest in III and AVF. A low-amplitude 7-to 10-Hz signal occurred in most leads. In the frontal plane, QRS axes and flow potential vectors were closely clustered. In the transverse plane, QRS axes generally followed leads V5 or V6, whereas the flow potential vectors followed leads V1, V2, or V3. The normal and patient groups did not differ. Although leads III and AVP showed the smallest superimposed potentials, V5, V6, or a left posterior chest lead may maximize QRS and reduce artifact most consistently. A 7- to 10-Hz frequency filter may help eliminate artifacts in some subjects.
Enhancing Accuracy in Molecular Weight Determination of Highly Heterogeneously Glycosylated Proteins by Native Tandem Mass Spectrometry (2017)

The determination of molecular weights (MWs) of heavily glycosylated proteins is seriously hampered by the physicochemical characteristics and heterogeneity of the attached carbohydrates. Glycosylation impacts protein migration during sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) and size-exclusion chromatography (SEC) analysis. Standard electrospray ionization (ESI)-mass spectrometry does not provide a direct solution as this approach is hindered by extensive interference of ion signals caused by closely spaced charge states of broadly distributed glycoforms. Here, we introduce a native tandem MS-based approach, enabling charge-state resolution and charge assignment of protein ions including those that escape mass analysis under standard MS conditions. Using this method, we determined the MW of two model glycoproteins, the extra-cellular domains of the highly and heterogeneously glycosylated proteins CD38 and epidermal growth factor receptor (EGFR), as well as the overall MW and binding stoichiometries of these proteins in complex with a specific antibody.
Vorhersage der Ligandenerkennung in einem Geruchsrezeptor durch Kombination von ortsgerichteter Mutagenese und dynamischer Homologie‐Modellierung (2011)

Der richtige Riecher: Durch dynamische Homologie-Modellierung konnte eine Liganden-Bindenische in einem dreidimensionalen Modell eines Geruchsrezeptors vorhergesagt werden. Die Vorhersage konnte experimentell durch Funktionsstudien an punktmutierten Rezeptormutanten bestätigt werden. Mithilfe des Modells ließen sich Bindungs- und Aktivierungseigenschaften des Rezeptors sogar gezielt umprogrammieren. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Beyond Spatial Auto-Regressive Models: Predicting Housing Prices with Satellite Imagery (2016)

When modeling geo-spatial data, it is critical to capture spatial correlations for achieving high accuracy. Spatial Auto-Regression (SAR) is a common tool used to model such data, where the spatial contiguity matrix (W) encodes the spatial correlations. However, the efficacy of SAR is limited by two factors. First, it depends on the choice of contiguity matrix, which is typically not learnt from data, but instead, is assumed to be known apriori. Second, it assumes that the observations can be explained by linear models. In this paper, we propose a Convolutional Neural Network (CNN) framework to model geo-spatial data (specifi- cally housing prices), to learn the spatial correlations automatically. We show that neighborhood information embedded in satellite imagery can be leveraged to achieve the desired spatial smoothing. An additional upside of our framework is the relaxation of linear assumption on the data. Specific challenges we tackle while implementing our framework include, (i) how much of the neighborhood is relevant while estimating housing prices? (ii) what is the right approach to capture multiple resolutions of satellite imagery? and (iii) what other data-sources can help improve the estimation of spatial correlations? We demonstrate a marked improvement of 57% on top of the SAR baseline through the use of features from deep neural networks for the cities of London, Birmingham and Liverpool.
Assembly of Colloidal Materials Using Bioadhesive Interactions (2002)

We have pursued the use of biological crosslinking molecules of several types to make colloidal materials at relatively low volume fraction of colloidal particles. The objective is to make binary alloys of colloidal particles, made of two different colloidal particles coated with complementary biological lock-and-key binding molecules, which assemble due to the biological specificity. The long-term goal is to use low affinity lock-and-key biological interactions, so that the can anneal to form crystalline states. We have used a variety of different surface chemistries in order to make colloidal materials. Our first system involved using selectin-carbohydrate (sialyl-Lewis) interactions; this chemistry is derived from immune system. This chemical interaction is of relatively low affinity, with timescales for dissociation of several seconds. Furthermore, the adhesion mediated by these molecules can be reversed by the chelation of calcium atoms; thus assembled structures can be disassembled reversibly. Our second system employed avidin-biotin chemistry. This well-studied system is of high affinity, and is generally irreversible on a laboratory time-scale. Thus, we would expect selectin-carbohydrate interactions at high molecular density and avidin-biotin interactions to give kinetically-trapped structures; however, at low densities, we would expect significant differences in the structure and dynamics of the two materials, owing to their very different release rates. We have also begun to use a third chemistry - DNA hybridization. By attaching single stranded DNA oligonucleotide chains to beads, we can drive the assembly of colloidal materials by hybridization of complementary DNA chains. It is well known that DNA adenosine-thymine (A-T) and guanine-cytosine (G-C) bases hybridize pairwise with a Gibbs free energy change of 1.7 kcal/mol per base; thus, the energy of the assembly can be modulated by altering the number of complementary bases in the DNA chains. Using these different crosslinking molecules, we have assembled colloidal materials from different-sized colloidal particles, A and B. In the first sets of experiment, we used high densities of adhesion molecules, and 0.96 micron (A) and 5.5 micron (B) diameter particles. The high density of adhesion molecules means that the structures are kinetically trapped in nonequilibrium configurations. The structure of the suspension can be varied by changing the number ratio of the two types of colloidal particles, NA and NB, where A is the smaller particle. With carbohydrate-selectin or avidin-biotin interactions, large NA/NB leads to the formation of colloidal micelles, with the large center B particle surrounded by many smaller A particles. As the ratio NA/NB decreases, the structures become more extended, approaching the formation of macro-Rouse polymers - extended linear chains where A beads are connected with intervening small B linkers.
Studies of Benzamide‐ and Thiol‐Based Histone Deacetylase Inhibitors in Models of Oxidative‐Stress‐Induced Neuronal Death: Identification of Some HDAC3‐Selective Inhibitors (2009)

We compare three structurally different classes of histone deacetylase (HDAC) inhibitors that contain benzamide, hydroxamate, or thiol groups as the zinc binding group (ZBG) for their ability to protect cortical neurons in culture from cell death induced by oxidative stress. This study reveals that none of the benzamide-based HDAC inhibitors (HDACIs) provides any neuroprotection whatsoever, in distinct contrast to HDACIs that contain other ZBGs. Some of the sulfur-containing HDACIs, namely the thiols, thioesters, and disulfides present modest neuroprotective activity but show toxicity at higher concentrations. Taken together, these data demonstrate that the HDAC6-selective mercaptoacetamides that were reported previously provide the best protection in the homocysteic acid model of oxidative stress, thus further supporting their study in animal models of neurodegenerative diseases.
Towards a framework for worm-defense evaluation (2006)

Computer worms are a serious problem. Much research has been done to detect and contain worms. One major deficiency in most research is that the claims are supported by theoretic models or simulations only and not by realistic tests. Network testbeds such as emulab and deter can be used to conduct worm experiments on networks of a few hundreds nodes. However, setting up such an experiment is not trivial. In this paper, we describe a wrapper around emulab to deploy such experiments quickly. We also demonstrate its use by evaluating an example worm containment strategy.
Catalytic synthesis of isoprenol from fatty acid ester over bimetallic Cu–Fe catalysts (2022)

Supported bimetallic Cu–Fe catalysts revealed high activity and selectivity in isoprenyl acetate hydrogenation to isoprenol under mild reaction conditions (2 MPa H2 and 170 °C). The nature of the carrier has a significant impact on the catalytic properties of Cu–Fe catalysts. The best catalytic properties were found for the 5% Cu–5% Fe/Al2O3 bimetallic catalyst, which provides a 98% isoprenyl acetate conversion in 4 h with the isoprenol selectivity of 82%.
Inline Coherent Imaging (2012)

Thesis (Ph.D, Physics, Engineering Physics and Astronomy) -- Queen's University, 2012-11-20 15:31:21.708
Synthesis of side chain liquid crystal polymers by living ring-opening metathesis polymerization. 4. Synthesis of AB block copolymers containing amorphous and side chain liquid crystal blocks (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of side chain liquid crystal polymers by living ring-opening metathesis polymerization. 4. Synthesis of AB block copolymers containing amorphous and side chain liquid crystal blocksZen Komiya and Richard R. SchrockCite this: Macromolecules 1993, 26, 6, 1387–1392Publication Date (Print):March 1, 1993Publication History Published online1 May 2002Published inissue 1 March 1993https://pubs.acs.org/doi/10.1021/ma00058a030https://doi.org/10.1021/ma00058a030research-articleACS PublicationsRequest reuse permissionsArticle Views352Altmetric-Citations78LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Uptake of prostate-specific membrane antigen-targeted 18F-DCFPyL in avascular necrosis of the femoral head (2019)

In recent years, the emergence of prostate-specific membrane antigen (PSMA)-targeted positron-emission tomography (PET) imaging has brought about a paradigm shift in the way that prostate cancer (PCa) is imaged in many parts of the world. Although PSMA-targeted PET imaging has been demonstrated to be a highly sensitive and specific imaging modality for the identification of sites of PCa, its clinical utility hinges on the ability of imaging specialists and their clinical colleagues to recognize potential false-positive sources of uptake and to tailor therapy based on that recognition. In this manuscript, we report the case of a 74-year-old male with a history of recurrent PCa who was referred for a restaging PSMA-targeted <sup>18</sup>F-DCFPyL PET/computed tomography (PET/CT). PET images demonstrated low level but focal and definitive uptake in the left femoral head. This uptake corresponded to sclerotic changes on CT whose morphology was most compatible with avascular necrosis without femoral head collapse. In the presented case, the integrated assessment of the CT imaging together with the PET findings was fundamental to avoid misinterpretation of the left femur finding as metastatic disease, which would have ultimately altered the clinical management of the patient.
Supplementary Figures 1-3 from hnRNP A2/B1 Modulates Epithelial-Mesenchymal Transition in Lung Cancer Cell Lines (2023)

Supplementary Figures 1-3 from hnRNP A2/B1 Modulates Epithelial-Mesenchymal Transition in Lung Cancer Cell Lines
Static and Fatigue Behavior of Spot-Welded 5182-0 Aluminum Alloy Sheet (1999)

There is a strong interest in the use of aluminum alloy sheet for vehicle applications, particularly the body, where resistance spot welding is the principal joining method. It is important that the particular discontinuities that are often found in aluminum alloy spot welds do not adversely affect the weld properties. The objectives of this work were to provide information about the effect of excessive porosity and surface indentation and the effect of weld size on the fatigue performance of spot welds in aluminum alloy sheet. Trials were conducted on 1.2-mm-thick 5182-0 aluminum alloy in the mill-finished condition. Static shear and fatigue tests were conducted on welds over a range of welding conditions to simulate severe weld discontinuities. The work indicated that nugget porosity, up to about 40% of the weld diameter, deep surface indentation and variation in weld size had no major impact on the fatigue properties of the welds.
Optical communication (2006)

LEDs are used in communication systems transmitting low and medium data rates (< 1 Gbit/s) over short and medium distances (< 10 km). These communication systems are based on either guided light waves (Keiser, 1999; Neyer et al., 1999; Hecht, 2001; Mynbaev and Scheiner, 2001; Kibler et al., 2004) or free-space waves (Carruthers, 2002; Heatley et al., 1998; Kahn and Barry, 2001). In guided-wave communication, individual optical fibers or fiber bundles are used as the transmission medium and LED-based optical communication links are limited to distances of a few kilometers. Optical fiber systems include silica and plastic optical fibers. Free-space communication is usually limited to a room, even though longer distances are possible. In this chapter we discuss the characteristics of transmission media used for LED communication.
Abstract 340: Myocyte-borne Bdnf is Essential to Limit Post-ischemic Cardiac Injury and Dysfunction (2020)

Brain-derived neurotrophic factor (BDNF)/ tyrosine receptor kinase B (TrkB) signaling is essential for normal cardiac contraction/relaxation. Alterations in this pathway, i.e., defective neuronal BDNF, account for post-ischemic cardiac injury. Less clear, however, is whether myocyte-borne BDNF has a role in this setting. We generated myocyte-selective BDNF knock-out (myoBDNF -/- ) mice, using Myh6-Cre mice crossed with BDNF floxed mice, confirming bdnf deletion via RT-PCR in isolated myocytes. Hearts from 12-wk old myoBDNF -/- mice and WT littermates underwent global ischemia (30 min) and reperfusion (2 h). At this age, the two strains had similar left ventricular (LV) sizes and fractional shortening [63±1.1 (WT) vs. 60±1.2% (myoBDNF -/- )]. At reperfusion, myoBDNF -/- hearts displayed larger infarct size compared to WT (38±3 vs. 14±2%, n=9, p&lt;.0001) and worsened LV functional recovery ( Fig. 1 ). For example, the rate-pressure product recuperated only by 14±1.5 in myoBDNF -/- mice vs. 36±3% in WT (p&lt;.0001). The two groups had similar heart rates at 2 h reperfusion, however myoBDNF -/- mice markedly lost their contractility [dP/dt max = 436±58 vs. 1407±142 mmHg/sec (WT), p&lt; .0001], likely due to the exacerbated myocyte loss. Accordingly, post-ischemic troponin I release was significantly higher in myoBDNF -/- than in WT mice (0.9±0.04 vs. 1.3±0.04 ng/ml, p&lt;.0002). Thus, deleting bdnf in myocytes severely limits recovery after ischemia, directly linking myocyte-borne BDNF to the heart response to injury. Therefore, preserving or enhancing autologous myocyte BDNF generation offers new avenues to counter cardiac ischemic injury and subsequent heart failure progression.