gebhart/patrepeval huggingface dataset:
Abstract 532: GRK4 Palmitoylation is Necessary for Membrane Association and Dopamine-1 Receptor Activity (2014)

The renal dopaminergic system regulates a large percentage of renal sodium excretion under sodium loaded conditions; however, not much is known about intracellular dopaminergic regulatory mechanisms. The expression of the principal sodium regulatory dopaminergic receptor (the D1R) is negatively modulated by the G protein linked receptor kinase type 4 (GRK4) through phosphorylation while the D1R is expressed in the plasma membrane. Therefore, we hypothesized that mechanisms associated with GRK4 membrane localization might be associated with its activity. Using human renal proximal tubule cell lines (RPTC), we previously demonstrated that GRK4 is palmitoylated using 17-octadecynoic acid (17-ODYA) and click chemistry and that fenoldopam (FEN, D1R agonist) decreased palmitoylation only in normal cAMP-coupled RPTC. Here we expand on those studies by showing that GRK4 palmitoylation was inhibited by 2-bromopalmitate (2BP, 100 μmol/L, palmitoylation inhibitor) which shifted GRK4 expression from the membrane to the cytoplasm. We used two different techniques to show loss of GRK4 membrane association when GRK4 palmitoylation was inhibited. The first technique involved classic cell fractionation using ultracentrifugation and western blot identification of GRK4 in membrane protein fractions (VEH 0.174±0.023 RFU, 2BP 0.091±0.013 RFU, P&lt;0.05, N=3) The second technique utilized a novel membrane-associated fluorescent linked immunosorbent assay (mFLISA) to demonstrate the decrease in GRK4 membrane association (VEH 13,568±664 RFU, 2BP 5,155±974 RFU, P&lt;0.05, N=12). Additionally, we showed that inhibiting GRK4 palmitoylation ledto D1-like plasma membrane recruitment using a non-cell permeable fluorescent D1-like antagonist bodipy630 SKF83566 (VEH 58,242±3858 RFU, 2BP 80,153±4061 RFU, P&lt;0.01, N=6). In summary, we demonstrated a novel D1R regulatory mechanism through GRK4 palmitoylation, affecting its localization and activity.
ChemInform Abstract: Synthesis and Conformational Analysis of Torsionally Constrained 1,3,2‐ Dioxaphosphepanes (IV), (VII), (IX). (1992)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Blind adaptive interference suppression for the near-far resistant acquisition and demodulation of direct-sequence CDMA signals (1997)

Two key operations required of a receiver in a direct-sequence (DS) code division multiple access (CDMA) system are the timing acquisition of transmissions that are starting up or have lost synchronization, and the demodulation of transmissions that have been acquired. The reliability of both these operations is limited by multiple-access interference, especially for conventional matched filter-based methods, whose performance displays an interference floor and is vulnerable to the near-far problem. Recent work has shown that, provided timing information is available for a given transmission, it can be demodulated reliably using blind or training-sequence-based adaptive interference suppression techniques. These techniques are near-far resistant, unlike the matched filter demodulator, and do not require explicit knowledge of the interference parameters, unlike nonadaptive multiuser detectors. In this paper, we present a blind adaptive interference suppression technique for joint acquisition and demodulation, which has the unique feature that the output of the acquisition process is not simply the timing of the desired transmission, but a near-far resistant demodulator that implicitly accounts for knowledge of the timing and amplitudes of all transmissions to suppress the multiple-access interference. The only knowledge required by the scheme is that of the desired transmission's signature sequence, so that it is amenable to a decentralized implementation. On the other hand, it can be efficiently implemented as a centralized scheme in which the bulk of the computations for the adaptation are common to all transmissions that need to be acquired or demodulated.
Systems analysis of MVA-C induced immune response reveals its significance as a vaccine candidate against HIV/AIDS of clade C (2012)

DOAJ is a unique and extensive index of diverse open access journals from around the world, driven by a growing community, committed to ensuring quality content is freely available online for everyone.
The use of ultrasonic energy for regeneration of reactive iron used in in situ remediation (1998)

Researchers from the University of Florida studied the application of ultrasonic energy to rejuvinate an iron surface for the purpose of enhancing/restoring the rate of trichloroethylene (TCE) degradation. Extensive batch studies were conducted to examine the impact of ultrasound on iron with various initial surface conditions such as acid washed or unwashed/as received. Rate constants were determined both before and after ultrasound application. Results indicate that a sonication period as brief as 25 minutes has a significant positive impact on the first order rate constant for TCE degradation to nonchlorinated species. While acid washed iron exhibited a small increase in the rate constant, unwashed/as received iron demonstrated a greater than 33% increase suggesting that ultrasound may be a candidate technique for restoring iron activity.
MOF-positioned polyamide membranes with a fishnet-like structure for elevated nanofiltration performance (2019)

Fabrication of MOF functionalized PA membranes with crumpled fishnet-like structures used for nanofiltration with rapid water transport and superior mono/divalent salt selectivity.
Heterogeneous Integration of Precise and Approximate Storage for Error-Tolerant Workloads (2022)

This study proposes a heterogeneous integration of precise and approximate storage in data center storage. The storage control engine allocates precise and error-tolerant applications to precise and approximate storage, respectively. The appropriate use of both precise and approximate storage is examined by applying a non-volatile memory capacity algorithm. To respond to the changes in application over time, the non-volatile memory capacity algorithm changes capacity of storage class memories (SCMs), namely the memory-type SCM (M-SCM) and storage-type SCM (S-SCM), in non-volatile memory resource. A three-dimensional triple-level cell (TLC) NAND flash is used as a large capacity memory. The results indicate that precise storage exhibits a high performance when the maximum storage cost is high. By contrast, with a low maximum storage cost, approximate storage exhibits high performance using a low bit cost approximate multiple-level cell (MLC) S-SCM.
Scattering of Elastic Waves by Complex Defects; Multiple Scattering Formalism (1981)

Scattering of elastic waves by complex defects is investigated. In previous work the Distorted Wave Born Approximation was developed, in which a general shaped defect is represented as a sphere S and a remainder volume R, the latter being treated in first order perturbation theory. The DWBA was used to study scattering by a sequence of defects representing increasingly larger deviations from spherical; comparisons with experiment and other theories were made. This approach breaks down when scattering by R is of similar magnitude as by S. Such is the case for complex defects such as two spheres, or a sphere and a crack emanating from it. To treat such situations, a multiple scattering formalism was developed.
Structured discriminative models using deep neural-network features (2015)

State-of-the-art speech recognisers employ neural networks in various configurations. A standard (hybrid) speech recogniser computes the likelihood for one time frame and state, using only one out of thousands of possible neural-network outputs. However, the whole output vector carries information. In this paper, features from state-of-the-art speech recognisers are collected per phone given a particular context, and input to a discriminative log-linear model. The log-linear model is trained with conditional maximum likelihood or a large-margin criterion. A key element is the prior on the parameters of the log-linear model. The mean of the prior is set to the point where the performance of the original systems is attained. The log-linear model then provides an additional increase over the state-of-the-art performance of the individual systems.
Transformation of Escherichia coli with foreign DNA by electroporation. (1993)

Electroporation has been widely applied in molecular biology in recent years. In this study, successful transformation of plasmid DNA and transfection of phage DNA into E. coli were described by using intense electrical field of exponential decay waveform (electroporation) generated by a Gene Pulsar LN-101 (made in Tianjin, Tianjin Institute of Technology and Nankai University). We have obtained 10(9)-10(10) transformants/micrograms with strain DH5 alpha and plasmid pUC18, by a single voltage pulse at 1.0-2.5 kV (initial electric field strength = 2.8-16 kV/cm) with 5-20 microF capacitor. The efficiency of electroporation depends on various parameters: the electric field strength, capacitance, the pulse length (RC time constant), etc. The frequency of transformation was a linear function of the DNA concentration, as well as the density of recipient cell. The incubation time of before or after electroporation has no significant effect on transformation. The high transfection efficiency was also achieved with strain JM109 and M13mp19RF by electroporation. The method was easier, more time-saving and more efficient than Ca(2+)-dependent transformation (transfection) method. The results obtained by Gene-Pulsar LN-101 was similar to that by the Bio-Rad Gene-Pulsar apparatus.
In search of a sharper line focus: Rotating the étendue (2022)

Conventional line-focus solar concentrators are limited by the 2D concentration limit, two orders of magnitude lower than the three-dimensional limit. This leads to low concentration ratios and strict manufacturing tolerances. It has been shown that by eliminating the continuous translational symmetry of these systems, it is possible to go beyond the 2D limit while maintaining the linear geometry of a line focus. We demonstrate that one way to break this symmetry is through ´etendue rotation, and we present two new concentrator configurations based on this insight. The first configuration uses an ´etendue rotating retroreflector array to boost the concentration of a parabolic trough. Ray-tracing simulations show that this configuration can achieve very high geometric concentration ratios or very high acceptance angles (1484 x at &plusmn;9mrad acceptance angle, or 25 x at &minus;70mrad). However, this configuration requires two-axis external solar tracking. To get around this, we demonstrate a second configuration that uses an ´etendue rotating lens array with tracking integration. We demonstrate a design that achieves a geometric concentration of 146x at a &plusmn;9mrad, with a simulated average yearly efficiency of 94.9% when used with conventional horizontal single-axis external tracking at an installation latitude of 30&deg;. The extra constraints of the tracking integration gives this design a more modest concentration ratio, but it is still higher than the 2D concentration limit and more than three times as high as the concentration of a parabolic trough evaluated under the same conditions. We believe that these new configurations show that the design landscape for line-focus solar concentrators can be widened, and that a practical high-concentration line-focus concentrator may be within reach.
Safety, tolerability, pharmacokinetics and pharmacodynamics of VTP-43742, a RORγt inhibitor, in normal healthy volunteers (2016)

Abstract VTP-43742 is an orally active RORγt inhibitor being developed for the treatment of autoimmune disorders, including psoriasis, through inhibition of IL-17A production and down regulation of the IL-23 receptor. Safety, tolerability, pharmacokinetic (PK) and pharmacodynamic (PD) profiles of VTP-43742 were assessed in two, randomized, double-blind, placebo-controlled studies in which VTP-43742 was administered to normal healthy volunteers. The first study was a single ascending dose (SAD) study (N = 53) in which participants were randomized to a one-time administration of VTP-43742 (7 dose levels, 30–2000 mg) or placebo. The second study (NCT02555709) was a multiple ascending dose (MAD) study in which participants (N = 40) received VTP-43742 (5 dose levels, 100–1400 mg/d) or placebo once daily for 10 days. VTP-43742 was shown to be safe and generally well tolerated at all dose levels in both studies. No serious adverse events were reported, and all study subjects completed dosing. No clinically significant clinical chemistry, hematologic or ECG abnormalities were observed, and dose proportionality was demonstrated across all dose levels. In the SAD study, VTP-43742 had a terminal plasma half-life of ~30 hours. In an ex vivo whole blood assay (WBA) of IL-17A secretion, VTP-43742 suppressed RORγt dependent secretion of IL-17A in a dose-responsive manner; &amp;gt;90% inhibition was observed at the higher doses which was mostly maintained over 24 hours. In the MAD study, those receiving VTP-43742 showed &amp;gt;90% inhibition of RORγt dependent IL-17A secretion in the WBA for a full 24hrs in all but the lowest dose cohort. These data support the further development of VTP-43742 for the treatment of autoimmune disorders.
Design of integrated thin film coolers (2003)

Thin film coolers can be monolithically integrated with optoelectronic and high-speed electronic components. Important parameters in the design of such coolers are investigated theoretically and experimentally. A three-dimensional finite element simulator (ANSYS) is used to model self consistently thermal and electrical properties of a complete device structure. Heat conduction, Joule heating, thermoelectric and thermionic cooling are included as well as non ideal effects such as contact resistance, finite thermal resistance of the substrate and the heat sink, and heat generation in the wire bonds. Various substrate materials (InP, Si, Cu, diamond) and thicknesses are studied as well as the effect of current spreading in different sample geometries. Comparisons to experimental results with InGaAsP-based thermionic coolers are made. Simulations demonstrate that single stage thin film coolers can provide up to 20-30 degrees centigrade cooling with cooling power densities of several 1000's W/cm/sup 2/.
Analysis of period-1 passive limit cycles for flexible walking of a biped with knees and point feet (2015)

SUMMARY In this paper, we investigate dynamic walking as a convergence to the system's own limit cycles, not to artificially generated trajectories, which is one of the lessons in the concept of passive dynamic walking. For flexible walking, gait transitions can be performed by moving from one limit cycle to another one, and thus, the flexibility depends on the range in which limit cycles exist. To design a bipedal walker based on this approach, we explore period-1 passive limit cycles formed by natural dynamics and analyze them. We use a biped model with knees and point feet to perform numerical simulations by changing the center of mass locations of the legs. As a result, we obtain mass distributions for the maximum flexibility, which can be attained from very limited location sets. We discuss the effect of parameter variations on passive dynamic walking and how to improve robot design by analyzing walking performance. Finally, we present a practical application to a real bipedal walker, designed to exhibit more flexible walking based on this study.
Nanoscale tuneable light source (2009)

We report on a new type of optical source, a 'light-well', which can be used as a nanoscale tuneable emitter. Optical and plasmonic radiation is generated as an electron beam passes through a channel in a periodically layered metal-dielectric nanostructure. With a lateral size of just a few hundred nanometers, and an emission intensity of ~200 W/cm&lt;sup&gt;2&lt;/sup&gt; such light-wells may be employed in nanophotonic circuits as chip-scale free-electron light sources, or in densely packed ensembles for optical memory and display applications.
Atomic Scale Control of Spin Current Transmission at Interfaces (2022)

Ferromagnet/heavy metal bilayers represent a central building block for spintronic devices where the magnetization of the ferromagnet can be controlled by spin currents generated in the heavy metal. The efficiency of spin current generation is paramount. Equally important is the efficient transfer of this spin current across the ferromagnet/heavy metal interface. Here, we show theoretically and experimentally that for Ta as heavy metal the interface only partially transmits the spin current while this effect is absent when Pt is used as heavy metal. This is due to magnetic moment reduction at the interface caused by 3d-5d hybridization effects. We show that this effect can be avoided by atomically thin interlayers. On the basis of our theoretical model we conclude that this is a general effect and occurs for all 5d metals with less than half-filled 5d shell.
Solid‐Phase Synthesis of Glycopeptide Amides under Mild Conditions: Morphiceptin Analogues (1990)

Membrane glycoproteins belong to the most important compounds in living nature. The glycoprotein amides (morphiceptin analogous) have now become readily accessible by a solid-phase method (glycosyl is galacto- or glucopyranosyl). Upon detachment from the carrier, the sensitive glycosidic bond remains intact.
Precise resistance measurement of quantum anomalous Hall effect in magnetic heterostructure film of topological insulator (2020)

The accuracy of Hall resistance in the quantum anomalous Hall effect has been studied at zero magnetic field using Crx(Bi,Sb)2−x Te3-based magnetic heterostructure films of topological insulators. The measured deviation of the Hall resistance from its theoretical value h/e2 was less than 2 ppm when the source drain current was 30 nA. This result has verified that the quantization of the Hall resistance is very accurate in the magnetic heterostructure films and in the previously reported uniformly doped films.
Residues 20, 22, and 26 determine the subtype specificities of staphylococcal enterotoxins C1 and C2 (1992)

Nonconserved residues of staphylococcal enterotoxin C1 (SEC1) were converted to their counterparts in SEC2. The mutants that resulted were examined for reactivity with monoclonal antibodies (MAbs). Substitution at position 20, 22, or 26 interfered with binding of an SEC1-specific MAb. SEC1 mutants with substitutions at all three positions reacted only with an SEC2-specific MAb. Antibody-binding patterns were not associated with isoelectric point differences. All mutants retained biological activity.
Top-quark mass measurement in the all-hadronic t t ¯ $$ t\overline{t} $$ decay channel at s = 8 $$ \sqrt{s}=8 $$ TeV with the ATLAS detector (2017)

The top-quark mass is measured in the all-hadronic top-antitop quark decay channel using proton-proton collisions at a centre-of-mass energy of s = 8 TeV with the ATLAS detector at the CERN Large Hadron Collider. The data set used in the analysis corresponds to an integrated luminosity of 20.2 fb -1 . The large multi-jet background is modelled using a data-driven method. The top-quark mass is obtained from template fits to the ratio of the three-jet to the dijet mass. The three-jet mass is obtained from the three jets assigned to the top quark decay. From these three jets the dijet mass is obtained using the two jets assigned to the W boson decay. The top-quark mass is measured to be 173.72 0.55 (stat.) 1.01 (syst.) GeV.
Non-replicating rhabdovirus-derived particles (NRRPs) eradicate acute leukemia by direct cytolysis and induction of antitumor immunity (2013)

Rhabdoviruses (RVs) are currently being pursued as anticancer therapeutics for various tumor types, notably leukemia. However, modest virion production and limited spread between noncontiguous circulating leukemic cells requires high-dose administration of RVs, which exceeds the maximum tolerable dose of the live virus. Furthermore, in severely immunosuppressed leukemic patients, the potential for uncontrolled live virus spread may compromise the safety of a live virus approach. We hypothesized that the barriers to oncolytic virotherapy in liquid tumors may be overcome by administration of high-dose non-replicating RVs. We have developed a method to produce unique high-titer bioactive yet non-replicating rhabdovirus-derived particles (NRRPs). This novel biopharmaceutical is multimodal possessing direct cytolytic and immunomodulatory activity against acute leukemia. We demonstrate that NRRP resistance in normal cells is mediated by intact antiviral defences including interferon (IFN). This data was substantiated using murine models of blast crisis. The translational promise of NRRPs was demonstrated in clinical samples obtained from patients with high-burden multidrug-resistant acute myeloid leukemia. This is the first successful attempt to eradicate disseminated cancer using a non-replicating virus-derived agent, representing a paradigm shift in our understanding of oncolytic virus-based therapies and their application toward the treatment of acute leukemia.
Final Technical Report for SISGR: Ultrafast Molecular Scale Chemical Imaging (2017)

The Northwestern-Argonne SISGR program utilized newly developed instrumentation and techniques including integrated ultra-high vacuum tip-enhanced Raman spectroscopy/scanning tunneling microscopy (UHV-TERS/STM) and surface-enhanced femtosecond stimulated Raman scattering (SE-FSRS) to advance the spatial and temporal resolution of chemical imaging for the study of photoinduced dynamics of molecules on plasmonically active surfaces. An accompanying theory program addressed modeling of charge transfer processes using constrained density functional theory (DFT) in addition to modeling of SE-FSRS, thereby providing a detailed description of the excited state dynamics. This interdisciplinary and highly collaborative research resulted in 62 publications with ~ 48% of them being co-authored by multiple SISGR team members. A summary of the scientific accomplishments from this SISGR program is provided in this final technical report.
Design of a vapor transport deposition process for thin film materials (2006)

A vapor transport process for continuous deposition of elemental and compound thin film materials is presented. The process saturates a carrier gas with a vapor from a subliming source. The saturated mixture is directed over a substrate at lower temperature, resulting in a supersaturation condition and subsequent film growth. The process geometry, comprising the dimensions of the saturation and deposition zones, carrier gas pressure and flow rate, and saturation zone temperature are determined by calculating worst-case characteristic times and simply insuring that the residence time of the carrier gas sufficiently exceeds these times. A model was used to design a system, which is currently being used to deposit 1–10μm thick CdTe films on a 10×10cm2 translating substrate. The process produces film thickness uniformity to within ±5% in the translation direction and across the deposition zone, with a material utilization of 50%. Linear translation speed of 12.5cm∕min has been demonstrated in depositing a 4.5μm CdTe film. The vapor transport process has also been used to deposit CdxZn1−xTe alloy films over a wide range of compositions by addition of ZnTe to the source. Photovoltaic conversion efficiencies of &amp;gt;13% for CdTe and &amp;gt;12% for CdxZn1−xTe have been achieved by devices fabricated from vapor transport deposited films deposited on to moving CdS coated substrates. Refinements are suggested for commercial-scale deposition.