gebhart/patrepeval huggingface dataset:
The Geek Perspective: Answering the Call for Advanced Technology in Research Inquiry Related to Pediatric Brain Injury and Motor Disability (2017)

The Academy of Pediatric Physical Therapy Research Summit IV issued a Call to Action for community-wide intensification of a research enterprise in inquiries related to pediatric brain injury and motor disability by way of technological integration. But the barriers can seem high, and the pathways to integrative clinical research can seem poorly marked. Here, we answer the Call by providing framework to 3 objectives: (1) instrumentation, (2) biometrics and study design, and (3) data analytics. We identify emergent cases where this Call has been answered and advocate for others to echo the Call both in highly visible physical therapy venues and in forums where the audience is diverse.
Discriminant Analysis Based Prognostics of Avionic Systems (2007)

In this paper, a novel discriminant analysis based predictive model for preventing false alarms leading to unnecessary replacement of an avionic system component is presented. The model is validated by prediction of false alarms (also known as false positives, type I, or alpha errors) in the left generator shaft of a Sikorsky helicopter UH-60, using the Goodrich health and usage management system (HUMS). The paper presents one of the first approaches based on applying discriminant analysis for prognostics of avionic systems, specifically in the context of identifying false positives within the next 1 or 2 h. In practice, predictions for the next 2 h are sufficient as typical helicopter flight schedules and durations are such that up to 2 h advance notice is most useful. This is an important contribution because drive train components of helicopters are normally very robust with very rare failures; therefore, the cost of unnecessary preventive maintenance based on false alarms is very high.
Mitochondrial adenylate kinase (AK2) from bovine heart. The complete primary structure (1986)

The sequence analysis of adenylate kinase isoenzyme 2 (AK2) was completed using a gas-phase sequencer constructed in our laboratory. The enzyme contains 238 amino acid residues in the following order: (sequence; see text) The four cysteine residues of AK2 were reinvestigated. Cys-41 and Cys-233 contain free thiols, which can be carboxymethylated in the intact protein without loss of enzymic activity. Chemical and model-building studies suggest that the pair Cys-43/Cys-93 forms a disulfide in native AK2. The relative molecular mass of AK2, as deduced from the sequence, is 26104. Other methods, including titration of -SH groups, sedimentation equilibrium ultracentrifugation and gel filtration yielded Mr values in the range from 26 000 to 31 500, each value depending on the respective method of determination. Bovine heart AK2 contains 44 residues more than the homologous isoenzyme AK1 (myokinase). As all but one single insertions and deletions cancel, the higher Mr of AK2 is due to 9 residues preceding the N terminus of AK1, a stretch of 30 residues in the middle of the molecule and 6 residues at the end. AK2 and AK1 are similar in their active-site geometry. In contrast, AK2 does not possess any of the three antigenic sites of AK1, which is consistent with the lack of immunological cross-reactivity between AK1 and AK2.
Novel quinazolinones and enniatins from Fusarium lateritium Nees (1994)

not available
Application of enzymes for press oil production from pumpkin seeds (2023)

Introduction.The aim of this research was to study the effect of pretreatment of pumpkin seed with proteolytic, cellulolytic, and pectolytic enzymes on pressed oil production.Materials and methods.Enzymes, papain, pectinase, cellulase, pepsin, and cellulolytic enzyme mixture Viscozyme L were used in the study.The number of disrupted cells was determined by the amount of easily extractable pumpkin seed oil as result of immediate shaking.To study the cell microstructure, ultramicrotome slices of pumpkin seeds were treated with individual enzymes and enzyme mixtures.The antioxidant activity of the pumpkin seed oil was determined using (DPPH) 2,2-diphenyl-1-picrylhydrazylradical radical scavenging activity.Results and discussion.The evaluation of cell integrity by immediate hexane extraction (shaking method) showed that all samples treated with different enzymes had a higher oil yield, ranging from 33.2 to 34.1% of seed weight, than the control samples (32.1%).The number of disrupted cells in enzyme treated samples was also higher than the control (64.4%), ranging from 67.6 to 69.5%.The highest amount of damaged cells, 71.0 and 71.1%, was found in the samples treated with mixtures (a) pepsin, Viscozyme L, pectinase and (b) pepsin, cellulase, with the pepsin+ViscozymeL+pectinase mixture was by 7.0% higher than that of the control sample.The enzymatic pretreatment did not significantly affect the free fatty acid content, peroxide value, fatty acid, and phytosterols composition.The antioxidant activity expressed as DPPH radical scavenging capacity of the pressed oil obtained from enzymatically pretreated pumpkin seeds was by 2.7% higher than of control oil.Conclusion.Pretreatment of pumpkin seeds with a mixture of cellulolytic and proteolytic enzymes allowed increasing the yield of oil with high quality characteristics.
Comprehensive Analysis of Human Endogenous Retrovirus Group HERV-W Locus Transcription in Multiple Sclerosis Brain Lesions by High-Throughput Amplicon Sequencing (2013)

Human endogenous retroviruses (HERVs) of the HERV-W group comprise hundreds of loci in the human genome. Deregulated HERV-W expression and HERV-W locus ERVWE1-encoded Syncytin-1 protein have been implicated in the pathogenesis of multiple sclerosis (MS). However, the actual transcription of HERV-W loci in the MS context has not been comprehensively analyzed. We investigated transcription of HERV-W in MS brain lesions and white matter brain tissue from healthy controls by employing next-generation amplicon sequencing of HERV-W env-specific reverse transcriptase (RT) PCR products, thus revealing transcribed HERV-W loci and the relative transcript levels of those loci. We identified more than 100 HERV-W loci that were transcribed in the human brain, with a limited number of loci being predominantly transcribed. Importantly, relative transcript levels of HERV-W loci were very similar between MS and healthy brain tissue samples, refuting deregulated transcription of HERV-W env in MS brain lesions, including the high-level-transcribed ERVWE1 locus encoding Syncytin-1. Quantitative RT-PCR likewise did not reveal differences in MS regarding HERV-W env general transcript or ERVWE1- and ERVWE2-specific transcript levels. However, we obtained evidence for interindividual differences in HERV-W transcript levels. Reporter gene assays indicated promoter activity of many HERV-W long terminal repeats (LTRs), including structurally incomplete LTRs. Our comprehensive analysis of HERV-W transcription in the human brain thus provides important information on the biology of HERV-W in MS lesions and normal human brain, implications for study design, and mechanisms by which HERV-W may (or may not) be involved in MS.
Performance results of a new DOI detector block for a high resolution PET-LSO research tomograph HRRT (1998)

To improve the spatial resolution and uniformity in modern high resolution brain PET systems over the entire field of view (FOV), it is necessary to archive the depth of interaction (DOI) information and correct for spatial resolution degradation. In this work we present the performance results of a high resolution LSO/GSO phoswich block detector with DOI capability. This detector design will be used in the new CTI High Resolution Research Tomograph, ECAT HRRT. The two crystal layers (19/spl times/19/spl times/7.5 mm/sup 3/) and a light guide are stacked on each other and mounted on a (2/spl times/2) PMT set, so that the corners of the phoswich are positioned over the PMT centers. The crystal phoswich is cut into an 8/spl times/8 matrix of discrete crystals. The separation of the LSO and the GSO layer by pulse shape discrimination allows discrete DOI information to be obtained. The high light output and the light guide design results in an accurate identification of the 128 single crystals per block. Flood source measurements document a very good homogeneity of events, energy centroid stability and energy resolution (14-20% FWHM) per single crystal. An intrinsic resolution of /spl sim/1.3 mm and the DOI feasibility is extracted by coincidence measurements with a single GSO crystal.
JAK1/STAT3 Activation through a Proinflammatory Cytokine Pathway Leads to Resistance to Molecularly Targeted Therapy in Non–Small Cell Lung Cancer (2017)

Molecularly targeted drugs have yielded significant therapeutic advances in oncogene-driven non-small cell lung cancer (NSCLC), but a majority of patients eventually develop acquired resistance. Recently, the relation between proinflammatory cytokine IL6 and resistance to targeted drugs has been reported. We investigated the functional contribution of IL6 and the other members of IL6 family proinflammatory cytokine pathway to resistance to targeted drugs in NSCLC cells. In addition, we examined the production of these cytokines by cancer cells and cancer-associated fibroblasts (CAF). We also analyzed the prognostic significance of these molecule expressions in clinical NSCLC samples. In NSCLC cells with acquired resistance to targeted drugs, we observed activation of the IL6-cytokine pathway and STAT3 along with epithelial-to-mesenchymal transition (EMT) features. In particular, IL6 family cytokine oncostatin-M (OSM) induced a switch to the EMT phenotype and protected cells from targeted drug-induced apoptosis in OSM receptors (OSMRs)/JAK1/STAT3-dependent manner. The cross-talk between NSCLC cells and CAFs also preferentially activated the OSM/STAT3 pathway via a paracrine mechanism and decreased sensitivity to targeted drugs. The selective JAK1 inhibitor filgotinib effectively suppressed STAT3 activation and OSMR expression, and cotargeting inhibition of the oncogenic pathway and JAK1 reversed resistance to targeted drugs. In the analysis of clinical samples, <i>OSMR</i> gene expression appeared to be associated with worse prognosis in patients with surgically resected lung adenocarcinoma. Our data suggest that the OSMRs/JAK1/STAT3 axis contributes to resistance to targeted drugs in oncogene-driven NSCLC cells, implying that this pathway could be a therapeutic target. <i>Mol Cancer Ther; 16(10); 2234-45. ©2017 AACR</i>.
Thermal degradation of sputtered Al/Ni(V)/Cu-UBM in Pb-free flip chip solder joints connected to substrate with different surface finishes (2008)

The thermal stability of flip chip solder joints made with Al/Ni(V)/Cu-UBM and SAC-405 solder bumps on substrates with either electroless Ni(P)-immersion gold (ENIG) or Cu surface finish (Cu-SOP) was determined at 170C. On ENIG, the resistance changed by more than one order of magnitude after 400 hours of high temperature storage, whereas on Cu-SOP, no change in resistance was observed up to 2400 hours of aging. The detailed electrical characterization of the solder joints was supplemented with time dependent physical characterization (microstructure and materials composition) of the joint using scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), focused ion beam microscopy (FIB), and transmission electron microscopy (TEM). The microstructural characterization clearly showed that electrical degradation of the ENIG devices was a direct result of the conversion and interfacial oxidation of the as deposited Ni(V)-barrier UBM layer into a porous layer that contained a web like fine grain V <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> Sn IMC. This conversion was driven by the Au from the ENIG surface finish. The results were similar to that reported by the authors for eutectic PbSn solder bumps, the exception being that for ENIG surface finish the resistance stability of SAC solder was 25x greater than eutectic that for PnSn solder.
Selenium‐sulfur analogs. Mass spectrometric comparison of substituted selenazoles and thiazoles (1981)

Abstract The electron impact mass spectra of a series of thiazoles and selenazoles were compared. No major differences in the mass spectral behavior between the sulfur and selenium containing compounds were observed. Isotopic labelling with deuterium permitted deconvolution of ion peaks of identical mass and composition, but arising from different fragmentation pathways.
Unconventional Pretreatment of Lignocellulose with Low‐Temperature Plasma (2016)

Lignocellulose represents a potential supply of sustainable feedstock for the production of biofuels and chemicals. There is, however, an important cost and efficiency challenge associated with the conversion of such lignocellulosics. Because its structure is complex and not prone to undergo chemical reactions very easily, chemical and mechanical pretreatments are usually necessary to be able to refine them into the compositional building blocks (carbohydrates and lignin) from which value-added platform molecules, such as glucose, ethylene glycol, 5-hydroxymethylfurfural, and levulinic acid, and biofuels, such as bioderived naphtha, kerosene, and diesel fractions, will be produced. Conventional (wet) methods are usually polluting, aggressive, and highly energy consuming, so any alternative activation procedure of the lignocellulose is highly recommended and anticipated in recent and future biomass research. Lignocellulosic plasma activation has emerged as an interesting (dry) treatment technique. In the long run, in particular, in times of fairly accessible renewable electricity, plasma may be considered as an alternative to conventional pretreatment methods, but current knowledge is too little and examples too few to guarantee that statement. This review therefore highlights recent knowledge, advancements, and shortcomings in the field of plasma treatment of cellulose and lignocellulose with regard to the (structural and chemical) effects and impact on the future of pretreatment methods.
[Hypotensive effect of a new calcium antagonist, SR 33557 in conscious rats]. (1990)

SR 33557 (SR) is a new calcium channel blocker belonging to the chemical class of indolizinsulfones (IC50 = 0.6 nM, 3H-nitrendipine). Its hypotensive effects were studied in conscious spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats, and compared to those of Nitrendipine (Nit) (IC50 = 0.8 nM, 3H-nitrendipine). SR was given intravenously (IV) at 0.3, 1 and 3 mg/kg (n = 4 to 6) and orally (PO) at 3, 10, 30 and 60 mg/kg (n = 4 to 7). Nitrendipine was studied at 0.3 mg/kg (IV) and 10 mg/kg (PO). Blood pressure (BP) and heart rate (HR) were measured for 120 min, and for 24 h at 30 mg/kg. The IV injection of SR induced an immediate and dose-dependent decrease in BP. The maximal diastolic hypotension was situated between 11 p. 100 at 0.3 mg/kg and 45 p. 100 at 3 mg/kg (basal values: 133 +/- 6 and 131 +/- 5 mmHg). These effects lasted between 30 min and over 2 hours. The oral administration of SR induced a dose-dependent fall in BP at 3 mg/kg and upwards. The maximal diastolic hypotension appeared within 60 and 120 min and were situated between 8 p. 100 at 3 mg/kg and 28 p. 100 at 60 mg/kg (basal values: 130 +/- 7 and 133 +/- 2 mmHg). At 30 mg/kg, the hypotension lasted for 8 hours. SR was roughly 10 times less hypotensive in WKY than in SHR. HR did not change.(ABSTRACT TRUNCATED AT 250 WORDS)
Sidewall morphology of electroformed LIGA parts-implications for friction, adhesion, and wear control (2005)

LIGA fabricated parts are finding application in a wide variety of micro-mechanical systems. For these systems to operate reliably, friction between contacting sidewall surfaces must be understood and controlled. The roughness of the as-plated sidewall is an important determinate of friction forces at such contacts. LIGA sidewalls were characterized in order to provide a basis for predicting the friction, adhesion, and wear behavior of LIGA micromachines. A variety of unexpected sidewall morphologies were observed during this investigation. Three morphologies were identified: a fine scale roughness, a linear through thickness feature, and a group of larger high aspect ratio features. Each morphology has been associated with a specific aspect of the LIGA manufacturing process. Potential friction, adhesion, and wear management strategies suggested by these features have been discussed. In addition, the asperity behavior in a LIGA sidewall contact has been predicted based on the finest roughness observed.
Spontaneous Rupture of Hydronephrotic Kidney during Pregnancy (1991)

Rupture of the urinary tract during pregnancy is uncommon. Pyelonephritis, painful overdistension and rupture are occasional effects of pregnancy on the urinary tract. We present a case of spontaneous rupture of a hydronephrotic kidney in a pregnant woman.
Photoinduced Current Transient Spectroscopy: Assessing the Impact of Defects on Lead‐Free Perovskite‐Inspired Photovoltaics via Photoinduced Current Transient Spectroscopy (Adv. Energy Mater. 22/2021) (2021)

In article number 2003968, Vincenzo Pecunia and co-workers demonstrate the wide applicability of photoinduced current transient spectroscopy (PICTS) for the characterization of defect levels in lead-free perovskite-inspired materials. In PICTS, light induces a mechanism through which carriers are captured by the defect levels, as if in a metaphorical perovskite-inspired billiard game. Due to its versatility, PICTS could catalyze the development of environmentally-friendly perovskite-inspired materials with benign defect properties for next-generation photovoltaics.
Impact of the Cu Current Collector Grade and Ink Solid Fraction on the Electrochemical Performance of Silicon‐Based Electrodes for Li‐Ion Batteries (2023)

The influence of the Cu current collector grade (cold‐rolled, annealed, or textured) and the ink solid fraction (SF) on the performance of Si‐based electrodes for Li‐ion batteries is investigated. Two binders are considered for this study: one of carboxymethyl cellulose and citric acid and the other which consists of the same organic components with added Zn(II) cations. These ones cross‐link the carboxylate moieties through coordination bonds. The fracture behavior and cyclability of the Zn‐free electrodes show a strong dependence on the Cu current collector grade. For this formulation, which has lower cohesion, the increase in electrode adhesion from the roughening of the surface of the current collector and/or the decrease of the current collector yield strength contribute to mitigate the electrode mechanical damage, therefore improving its cyclability. Such a dependence is not observed for the Zn cross‐linked electrodes which have a higher cohesion. Regarding the impact of the ink solid fraction, when a higher SF is used, the capacity retention and coulombic efficiency are enhanced for both formulations, though much more significantly for the Zn‐free formulation. The underlying mechanism causing this improvement may be partially related to the enhanced adsorption of binder on silicon particles with increasing SF.
Stable, Genetically Engineered F(ab′) 2 Fragments of Chimeric TNT-3 Expressed in Mammalian Cells (2002)

F(ab')(2) fragments are desirable structural derivatives of monoclonal antibodies (MAbs) because of their pharmacokinetic properties and bivalent binding to antigen. Production of these fragments, however, has proven difficult because of the variable sensitivity of intact antibodies to proteolytic enzymes, which can result in very low yields and unstable product. To circumvent these problems, we attempted to apply genetic engineering methods to generate stable F(ab')(2) fragments in NSO murine myeloma cells using the glutamine synthase expression system. For these studies, the chimeric MAb, chTNT-3, directed against necrotic regions of solid tumors, was used to generate several F(ab')(2) variants, which contained between one and three cysteine residues at the end of the hinge region. In addition, two different affinity tags (his tag, streptactin tag) were used with each variant to determine the best tag for purification procedures. Stability was measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and by antigen binding studies and the constructs were tested in vivo to measure their pharmacokinetic properties and biodistribution in normal organs and tumor. The results of these studies show that 3 cysteine residues are required to produce stable F(ab')(2) fragments and that either purification tag can be used with this variant to produce suitable reagents for in vivo studies. Those constructs containing one or two cysteines were found to be unstable and broke down to Fab fragments regardless of the purification tag used. These studies demonstrate that stable, clinically useful F(ab')(2) fragments of chTNT-3 can be produced in mammalian cells by genetic engineering methods.
Low Voltage Activation of KCa1.1 Current by Cav3-KCa1.1 Complexes (2013)

Calcium-activated potassium channels of the KCa1.1 class are known to regulate repolarization of action potential discharge through a molecular association with high voltage-activated calcium channels. The current study examined the potential for low voltage-activated Cav3 (T-type) calcium channels to interact with KCa1.1 when expressed in tsA-201 cells and in rat medial vestibular neurons (MVN) in vitro. Expression of the channel α-subunits alone in tsA-201 cells was sufficient to enable Cav3 activation of KCa1.1 current. Cav3 calcium influx induced a 50 mV negative shift in KCa1.1 voltage for activation, an interaction that was blocked by Cav3 or KCa1.1 channel blockers, or high internal EGTA. Cav3 and KCa1.1 channels coimmunoprecipitated from lysates of either tsA-201 cells or rat brain, with Cav3 channels associating with the transmembrane S0 segment of the KCa1.1 N-terminus. KCa1.1 channel activation was closely aligned with Cav3 calcium conductance in that KCa1.1 current shared the same low voltage dependence of Cav3 activation, and was blocked by voltage-dependent inactivation of Cav3 channels or by coexpressing a non calcium-conducting Cav3 channel pore mutant. The Cav3-KCa1.1 interaction was found to function highly effectively in a subset of MVN neurons by activating near -50 mV to contribute to spike repolarization and gain of firing. Modelling data indicate that multiple neighboring Cav3-KCa1.1 complexes must act cooperatively to raise calcium to sufficiently high levels to permit KCa1.1 activation. Together the results identify a novel Cav3-KCa1.1 signaling complex where Cav3-mediated calcium entry enables KCa1.1 activation over a wide range of membrane potentials according to the unique voltage profile of Cav3 calcium channels, greatly extending the roles for KCa1.1 potassium channels in controlling membrane excitability.