280 LIPOSOMAL TREATMENT OFFERS PROTECTION AGAINST ACROLEIN-INDUCED UROTHELIAL CELL DAMAGE (2013)

You have accessJournal of UrologyBladder and Urethra: Anatomy, Physiology and Pharmacology (I)1 Apr 2013280 LIPOSOMAL TREATMENT OFFERS PROTECTION AGAINST ACROLEIN-INDUCED UROTHELIAL CELL DAMAGE Jayabalan Nirmal, Amanda Wolf-Johnston, Michael Chancellor, Pradeep Tyagi, Jonathan Kaufman, Michele Anthony, and Lori Birder Jayabalan NirmalJayabalan Nirmal Royal Oak, MI More articles by this author , Amanda Wolf-JohnstonAmanda Wolf-Johnston Pittsburgh, PA More articles by this author , Michael ChancellorMichael Chancellor Royal Oak, MI More articles by this author , Pradeep TyagiPradeep Tyagi Pittsburgh, PA More articles by this author , Jonathan KaufmanJonathan Kaufman Pittsburgh, PA More articles by this author , Michele AnthonyMichele Anthony Pittsburgh, PA More articles by this author , and Lori BirderLori Birder Pittsburgh, PA More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.1664AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES A growing body of evidence supports the use of intravesical therapies for treatment of urinary bladder dysfunctions. Recent data has revealed that topical nanoparticle liposome treatment has a beneficial effect on reducing inflammation and irritation-induced bladder hyperactivity by forming a protective, urothelial coating. This study examined the mechanism underlying liposome uptake in urothelial cells and whether liposome treatment can reduce acrolein induced urothelial cell damage. METHODS Primary urothelial cells were cultured as previously described and protective effect of liposomes against acrolein (2 μM) exposure was assessed by: a) BrdU cell proliferation, b) cytokine production and c) trypan blue staining. This was determined in control cells (unexposed to acrolein), control cells pretreated with liposomes (2 mg/ml), cells exposed to acrolein (2 μM) and in urothelial cells treated with liposomes prior to acrolein exposure. The mechanisms underlying this cytoprotection were assessed by confocal microscopy of fixed urothelial cells treated with fluorescent-labeled liposomes. RESULTS Acrolein induced epithelial toxicity caused a reduction in cellular integrity (increased trypan blue staining), proliferation of urothelial cells (mean 43% decrease as compared with untreated control cells) and increased cytokine (IFN-γ) production, that was blocked or prevented by liposome pretreatment. Confocal microscopy was able to localize fluorescent-labeled liposomes outside the urothelial cell membrane as well as within the cytoplasm. CONCLUSIONS We demonstrated that liposomes increased the survival of urothelial cells exposed to acrolein and cell surface bound liposomes are internalized via endocytosis. Taken together, our findings suggest that liposomes may act to repair the urothelial barrier function and reduce inflammation. This could be important in pathologies such as ulcerative cystitis, a severe form of painful bladder syndrome that causes severe, chronic pelvic pain. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e114 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Jayabalan Nirmal Royal Oak, MI More articles by this author Amanda Wolf-Johnston Pittsburgh, PA More articles by this author Michael Chancellor Royal Oak, MI More articles by this author Pradeep Tyagi Pittsburgh, PA More articles by this author Jonathan Kaufman Pittsburgh, PA More articles by this author Michele Anthony Pittsburgh, PA More articles by this author Lori Birder Pittsburgh, PA More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...