gebhart/patrepeval huggingface dataset:
Sensitization of T Lymphocytes in Vitro by Syngeneic Macrophages Fed with Tumor Antigens (1976)

We investigated the interaction between T lymphocytes and macrophages in the vitro sensitization of lymphocytes against tumor cells. Spleen cells were sensitized in vitro by syngeneic peritoneal macrophages that had been fed with cell-free antigen preparation of syngeneic tumor cells. The sensitized T lymphocytes acquired specific cytotoxic cells. The sensitized T lymphocytes acquired specific cytotoxic activity in vitro and the capacity to inhingeneic fibroblasts, or the antigen preparation by itself were not able to sensitize the lymphocytes against the tumor.
Metabolomics-Based Identification of Metabolic Dysfunction in Frailty (2021)

Dysregulation of energy producing metabolic pathways has been observed in older adults with frailty. In this study, we used liquid chromatography-mass spectrometry technology to identify aging- and frailty-related differences in metabolites involved in glycolysis, the tricarboxylic (TCA) cycle, and other energy metabolism-related pathways in the serum of a cohort of community-dwelling adults aged 20-97 (n = 146). We also examined the relationship between serum levels of metabolites and functional measures, physical frailty, and risk status for adverse health outcomes. We observed elevated levels of TCA cycle and glycolytic intermediates in frail subjects; however, the differences in the levels of ATP and other energy metabolites between young, nonfrail, and frail adults were not significant. Instead, we found that serum levels of neurotransmitters N-acetyl-aspartyl-glutamate, glutamate, and γ-aminobutyric acid were significantly elevated in older adults with frailty. These elevations of glycolytic and TCA cycle intermediates, and neurotransmitters may be part of the biological signature of frailty.
Synaptotagmin-1 is a Ca2+ sensor for somatodendritic dopamine release (2022)

Modes of somatodendritic transmission range from rapid synaptic signaling to protracted regulation over distance. Somatodendritic dopamine secretion in the midbrain leads to D2 receptor-induced modulation of dopamine neurons on the timescale of seconds. Temporally imprecise release mechanisms are often presumed to be at play, and previous work indeed suggested roles for slow Ca<sup>2+</sup> sensors. We here use mouse genetics and whole-cell electrophysiology to establish that the fast Ca<sup>2+</sup> sensor synaptotagmin-1 (Syt-1) is important for somatodendritic dopamine release. Syt-1 ablation from dopamine neurons strongly reduces stimulus-evoked D2 receptor-mediated inhibitory postsynaptic currents (D2-IPSCs) in the midbrain. D2-IPSCs evoked by paired stimuli exhibit less depression, and high-frequency trains restore dopamine release. Spontaneous somatodendritic dopamine secretion is independent of Syt-1, supporting that its exocytotic mechanisms differ from evoked release. We conclude that somatodendritic dopamine transmission relies on the fast Ca<sup>2+</sup> sensor Syt-1, leading to synchronous release in response to the initial stimulus.
Wafer fusion: materials issues and device results (1997)

A large number of novel devices have been recently demonstrated using wafer fusion to integrate materials with different lattice constants. In many cases, devices created using this technique have shown dramatic improvements over those which maintain a single lattice constant. We present device results and characterizations of the fused interface between several groups of materials.
Finding nonrecursive envelopes for Datalog predicate (1993)

In this paper, we study the ability of data-independent conjunctive expressions (envelopes) to approximate fixpoint of Datalog predicates. We show that no effective procedure exists for finding envelopes that best approximate the fix-point (tight envelopes). Moreover, the problem of determining existence of tight envelopes is undecidable. The relationship between tight envelopes and the boundedness property is explored. Although the property of having tight envelopes seems weaker than boundedness, we note that a predicate can have a tight (lower) envelope iff it is bounded. On the other hand, there exist Datalog predicates that are not bounded but have tight (upper) envelopes. We relax our requirement for tight envelopes and settle for connected envelopes. An algorithm to determine connected envelopes for Datalog predicates is presented. We mention several applications of envelopes.
Potential energy surface of In and Ga adatoms above the (111)A and (110) surfaces of a GaAs nanopillar (2011)

Density-functional calculations of the potential-energy surface for tracer Ga and In adatoms above two GaAs (111)A and two GaAs (110) surface reconstructions are presented in order to understand the growth conditions required to form axial GaAs/InGaAs heterostructures in nanopillars. The surface reconstructions present under As-rich conditions have lower diffusion barriers for In adatoms. In addition, the binding energy of In becomes more competitive with Ga under As-rich conditions. We conclude that the As-rich reconstructions for GaAs(110) and GaAs(111)A are preferable for selective formation of heterointerfaces on (111) facets. This work helps explain the recent successful formation of axial GaAs/InGaAs heterointerfaces in catalyst free nanopillars.
(Invited) Thin Epitaxial Film of Ge and III-V Directly Bonded onto Si Substrate (2014)

We have realized patterned Ge-on-Insulator wafers by large-scale layer transfer technology. In conjunction with low-temperature bonding and patterned Epitaxial Lift-Off (ELO) technique, high quality Ge or III-V layer transfer was achieved in full-wafer scale. Furthermore, to expand the transferred area, multiple chip-to-wafer bonding and thin epitaxial film transfer has been demonstrated on 300 mm Si wafer. Low temperature layer transfer technology using ELO technique provide the flexible opportunity to elaborate Ge and III-V based substrates for post silicon CMOS as well as for monolithic heterogeneous integration in a cost effective manufacturing.
Platelet‐derived growth factor induces p21/WAF1 promoter in vascular smooth muscle cells via activation of an Sp1 site (2003)

Many studies suggested that cyclin-dependent kinase inhibitor (CDKI) p21 acts as a universal inhibitor of cyclin/CDK catalytic activity. This protein has also been shown to be a component of active cyclin/CDK complexes. In addition, it has recently been suggested that p21 serves as an assembly factor in platelet-derived growth factor (PDGF)-stimulated vascular smooth muscle cells (VSMC). However, little is known concerning the molecular mechanisms by which PDGF induces p21 gene expression in VSMC. In this report we demonstrate that PDGF induces the p21 expression at both the mRNA and protein levels. This increase in p21 gene expression was due to activation of the p21 promoter by PDGF. Through both deletion and mutation analysis of the p21 promoter, we defined a 10-bp sequence that is required for the activation of the p21 promoter by PDGF. In addition, gel shift and supershift assays demonstrated that this PDGF-responsive element binds specifically to the transcription factor Sp1. These results demonstrate that Sp1 mediates PDGF-induced p21 gene expression in VSMC. Moreover, immunoblot and immunoprecipitation analysis showed that the level of hyperphosphorylated retinoblastoma protein (Rb) is increased and the protein is physically associated with Sp1 in PDGF-treated cells, indicating that phosphorylated Rb may play a role in regulating Sp1 to activate p21 expression.
The Multimode LC Alignment on the Substrates Obliquely Treated with a Plasma Flux (2005)

ABSTRACT We describe a variety of liquid crystal (LC) alignment modes on the aligning substrates obliquely treated with a flux of accelerated plasma from the anode layer thruster (ALT). The discharge area has a form of race track so that the generated flux contained two sheets of plasma corresponding to linear parts of the discharge. Within each “sheet” the intensity of flow (ion current density) is uniform in the direction parallel to the linear part of discharge. Across the “sheet”, the intensity of flow has the Gaussian distribution. The alignment of LC with positive and negative dielectric anisotropy, Δϵ, was tested on the substrates of organic (polymers) and inorganic (glass and DLC) origin treated in static and dynamic regime (unidirectional translation perpendicularly to plasma “sheet”). In the dynamic regime of irradiation, with the substrate moving with respect to the plasma beam, for LC with Δϵ > 0 we observed two aligning modes, with the easy axis confined to the plane of plasma incidence (mode 1) or perpendicular to this plane (mode 2). In the 1st aligning mode the LC pretilt angle is non-zero and can be varied with the incidence angle of plasma beam and the irradiation dose. With the increase of irradiation dose, the alignment transition from the 1st to the 2nd mode occurs. For LC with Δϵ < 0 only the 1st alignment mode uniform over all substrate was observed. In the static regime of irradiation, characterized by smooth distribution of the particle's flow across the sheet, two modes are clearly observed simultaneously; 2nd mode alignment occurs in central, most intensive part of flow, while the periphery part shows the 1st mode. The areas of the 1st and the 2nd mode are separated with the narrow transition area showing multidomain (two-fold degenerate) LC alignment. The easy axis in these domains is turned by about ±45° with respect to the alignment direction in the 1st mode area. This sequence of the alignment transitions is observed in both LC with Δϵ > 0 and Δϵ < 0. We also describe a high-tilt alignment (87°–90°) in the 1st mode realized on the hydrophobic polymers at the extremely low doses of plasma irradiation. The origin of the observed alignment effects is discussed.
Inside Cover: Thiols and Selenols as Electron‐Relay Catalysts for Disulfide‐Bond Reduction (Angew. Chem. Int. Ed. 49/2013) (2013)

The maintenance of a particular redox state is critical for the proper function of many molecules, including proteins. In their Communication on page 12901 ff., R. T. Raines et al. report that a soluble mixed diselenide can catalyze the efficient relay of electrons from an immobilized dithiol to a protein in an aqueous solution. This system provides a simple means to reduce undesired disulfide bonds as well as to deter oxidative damage. (Graphic by S. B. Johnston and R. T. Raines)
Defects and electronic transport in hydrogenated amorphous SiC films of interest for low dielectric constant back end of the line dielectric systems (2013)

Back end of line dielectrics with low dielectric constants are needed for current and future integrated circuit technology. However, an understanding of the defects that cause leakage currents and limit reliability in these films is not yet developed. We utilize conventional electron paramagnetic resonance (EPR), electrically detected magnetic resonance (EDMR), and leakage current measurements, complimented by Fourier transform infrared spectroscopy and Rutherford back scattering results, to investigate a-SiC:H dielectrics which have great potential use for back end of line dielectrics. We observe a strong correlation between conventional EPR defect density measurements and leakage currents. There is also a very strong correlation between hydrogen content and both leakage current and EPR defect density. The close correspondence between the EPR results and the leakage currents strongly indicates that the defects observed by EPR are largely responsible for the leakage currents and likely limit the dielectric reliability. Important reliability phenomena in these films are time-dependent dielectric breakdown and stress induced leakage current. Additionally, we have utilized EDMR via spin dependent trap assisted tunneling to investigate the direct link between defects observed in EPR and electrical transport.
De Novo Prediction of P-Glycoprotein-Mediated Efflux Liability for Druglike Compounds (2012)

P-glycoprotein (Pgp) is capable of recognizing and transporting a wide range of chemically diverse compounds in vivo. Overcoming Pgp-mediated efflux can represent a significant challenge when penetration into the central nervous system is required or within the context of developing anticancer therapies. While numerous in silico models have been developed to predict Pgp-mediated efflux, these models rely on training sets and are best suited to make interpolations. Therefore, it is desirable to develop ab initio models that can be used to predict efflux liabilities. Herein, we present a de novo method that can be used to predict Pgp-mediated efflux potential for druglike compounds. A model, which correlates the computed solvation free energy differences obtained in water and chloroform with Pgp-mediated efflux (in logarithmic scale), was successful in predicting Pgp efflux ratios for a wide range of chemically diverse compounds with a R(2) and root-mean-square error of 0.65 and 0.29, respectively.
Abstract PR14: Ewing sarcoma cells exploit the IL1RAP-CTH axis to drive oxidative stress adaptation and lung metastasis (2020)

Abstract Metastasis is a highly inefficient process due to the high oxidative stress that cancer cells experience, such as in the circulation and at distant organs prior to colonization. How cancer cells adapt to those stressful conditions and develop anti-anoikis properties remains elusive. Here our global translatome and proteome analyses have uncovered novel signatures exploited by oncogene-transformed cells to adapt and survive anoikis-inducing stress. We reveal that EWS-ETS chimeric oncoproteins trigger the expression of the IL1RAP surface protein to alleviate oxidative stress and facilitate stress adaptation, thereby promoting lung metastasis in Ewing sarcoma. Proteomic profiling identifies CTH, a key enzyme for de novo cysteine synthesis and redox regulation, as an important functional downstream mediator of IL1RAP. Blockade of either IL1RAP or CTH, using genetic or pharmacologic approaches, renders EWS cells susceptible to oxidative stress in vitro, and dramatically mitigates primary tumor growth, local invasion and lung metastasis in mice. Importantly, high expression of this IL1RAP-CTH axis correlates with markedly decreased overall and event-free survival in EWS patients. Thus, our study has defined a novel mechanism exploited by EWS cells for metastasis, which can be a potential therapeutic target for high-risk metastatic EWS. This abstract is also being presented as Poster B58. Citation Format: Haifeng Zhang, Amal M. El-Naggar, Hongwei Cheng, Alberto Delaidelli, Gian Luca Negri, Wei Li, Dimiter S. Dimitrov, Poul H.B. Sorensen. Ewing sarcoma cells exploit the IL1RAP-CTH axis to drive oxidative stress adaptation and lung metastasis [abstract]. In: Proceedings of the AACR Special Conference on the Advances in Pediatric Cancer Research; 2019 Sep 17-20; Montreal, QC, Canada. Philadelphia (PA): AACR; Cancer Res 2020;80(14 Suppl):Abstract nr PR14.
Design and implementation of the Front End Board for the readout of the ATLAS liquid argon calorimeters (2008)

Buchanan, N J; Chen, L; Gingrich, D M; Liu, S; Chen, H; Farrell, J; Kierstead, J; Lanni, F; Lissauer, D; Ma, H; Makowiecki, D; Radeka, V; Rescia, S; Takai, H; Ghazlane, H; Hoummada, A; Wilkens, H G; Bán, J; Böttcher, S; Brooijmans, G; Chi, C-Y; Caughron, S; Cooke, M; Dannheim, D; Gara, A; Haas, A; Katsanos, I; Parsons, J A; Simion, S; Sippach, W; Zhang, L; Zhou, N; Ladygin, E; Augé, E; Bernier, R; Bouchel, M; Bozzone, A; Breton, D; de La Taille, C; Falleau, I; Imbert, P; Martin-Chassard, G; Perus, A; Richer, J P; Tocut, V; Veillet, J-J; Zerwas, D; Hubaut, F; Laforge, B; LeDortz, O; Martin, D; Schwemling, Ph; Collot, J; Dzahini, D; Gallin-Martel, M-L; Martin, P; Cwienk, W D; Fent, J; Kurchaninov, L; Battistoni, G; Carminati, L; Citterio, M; Cleland, W; Liu, B; Rabel, J; Zuk, G; Benslama, K; Delagnes, E; Mansoulin, B; Teiger, J; Dinkespiler, B; Liu, T; Stroynowski, R; Yang, C A; Ye, J; Chu, M L; Lee, S -C; Teng, P K
Modeling Nonlinearity in Coherent Transmissions with Dominant Interpulse-Four-Wave-Mixing (2011)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text A. Bononi, E. Grellier, P. Serena, N. Rossi, and F. Vacondio, "Modeling Nonlinearity in Coherent Transmissions with Dominant Interpulse-Four-Wave-Mixing," in 37th European Conference and Exposition on Optical Communications, OSA Technical Digest (CD) (Optica Publishing Group, 2011), paper We.7.B.4. Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
A cellular 3,3',5-triiodo-L-thyronine binding protein from a human carcinoma cell line. Purification and characterization. (1987)

A cellular binding protein for 3,3',5-triiodo-L-thyronine (T3) was solubilized with 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) from A431 human epidermoid carcinoma cells. The binding activity is T3 specific. Analysis of the equilibrium binding data indicated that the binding protein has one class of binding sites for T3 with a Kd of (17 +/- 3) nM and Bmax of (1.8 +/- 0.6) pmol/50 micrograms of protein. The pH optimum for binding is 6.8. The T3 binding protein elutes from Sephadex G-200 in an included peak which has a Stokes radius of 40 A and sediments on glycerol gradients at 3.7 S. By affinity labeling with [3,5-125I]thyroxine a protein with a molecular weight of 58,000 was specifically labeled. Its isoelectric point was determined to be 7.1, which is different from the reported pIs of other thyroid hormone binding proteins. p58 was successively purified to apparent homogeneity by chromatography on Sephadex G-200, QAE-Sephadex, SP-Sephadex, and hydroxylapatite. Approximately 50 micrograms of purified protein was obtained from 2.5 X 10(9) cells with a yield of 1.1%. The purified protein retains its binding activity. The specific binding activity is enriched by approximately 1000-fold. With the availability of a purified protein with T3 binding activity, it becomes possible to study its cellular function.
SCLERA: an astrometric telescope for experimental relativity. (1974)

An f/100, 12.2-m focal length photoelectric telescope designed specifically for daytime astrometry of objects near the sun is now operative at its Tucson, Arizona, site. The design goal was to achieve accuracies of order 0.001 sec of arc in field position measurements of stars. To accomplish this, many features reducing systematic and random errors are employed, including Schupmann medial telescope optics, compensation for lateral color aberration, apodization for reduction of diffracted light, and use of an accurately measured solar diameter for calibrating the field.
First Experiments on Neutral Injection in Multimirror Trap GOL-3 (2009)

AbstractFirst stage of neutral beam heating system was developed at the GOL-3 facility. Configuration of magnetic field and initial gas distribution were adopted for operation with NBI system. As a result the neutral beam with 0.5 MW power and 0.8 ms pulse duration was achieved and stable regimes suitable for beam injection experiments were found. The neutral beam system was used in first experiment as diagnostic tool for measuring the plasma density. Dynamics of plasma density and its radial distribution were studied in experiments.
The Different Binding Patterns of Two Immunoglobulin M Monoclonal Antibodies toCryptococcus neoformansSerotype A and D Strains Correlate with Serotype Classification and Differences in Functional Assays (1998)

Cryptococcus neoformans var. neoformans strains have historically been divided into serotypes A and D on the basis of reactivity with rabbit sera. Previously, we noted that two murine immunoglobulin M monoclonal antibodies (MAbs) to the capsular glucuronoxylomannan produced different indirect immunofluorescence (IF) patterns, described as annular and punctate, when bound to C. neoformans cells from different strains. In this study, we examined the reactivity of these two MAbs, known as 12A1 and 13F1, with 20 C. neoformans var. neoformans strains, of which 13 were serotype A and 7 were serotype D. For all strains, MAb binding was studied by IF and agglutination assays. In addition, we blindly tested the IF patterns of 22 C. neoformans var. neoformans strains. For selected strains, MAb binding was studied by flow cytometry (FACScan) and phagocytosis assays. The epitopes recognized by MAbs 12A1 and 13F1 were found in all of the strains. MAb 12A1 binding produced an annular IF pattern with all of the strains, irrespective of the serotype classification. MAb 13F1 binding produced annular binding with all of the serotype A strains and punctate binding with 19 of 20 serotype D strains. In general, the punctate IF pattern was associated with lower fluorescence intensity, a requirement for higher antibody concentrations to produce yeast cell agglutination, and lower opsonic efficacy. Our results provide strong support for the existing classification of two serological types for strains assigned to variety neoformans and indicate qualitative and quantitative antigenic differences among serotype A and D strains.
Competitive and positional cues in the patterning of nerve connections (1990)

The visual system of lower vertebrates has served as an important testing ground for the mechanisms that generate topographic neuronal connections. During both the outgrowth and the regeneration of the optic nerve, a smoothly ordered map of the retina is formed on its major target, the optic tectum (the retinotectal projection). Experiments performed on this projection have offered support for a variety of mechanisms, including the matching of positional cues in the retina and tectum, the guidance of nerve fibers by interactions between fibers, competition for synaptic space, and the refinement of connections based on neuronal activity. Unfortunately, individual experiments that support any one of these mechanisms have been taken at times as evidence against the involvement of any other mechanism; for example, experiments demonstrating the importance of positional cues have been thought mistakenly to indicate that activity-based interactions are unimportant. Computer simulations, in which multiple, somewhat opposed, mechanisms are allowed to operate in concert demonstrate that such a hybrid model is able to generate a full range of experimental results. More importantly, the elimination of any one of the mechanisms renders the model unable to fit entire classes of findings. Thus, the patterning of the retinotectal projection is best viewed as a process in which the optic nerve terminals attempt to satisfy multiple constraints in selecting their target sites.
The impact of the deposition process of the emitting layer on the internal structure of organic light-emitting diodes (2019)

Abstract Organic light-emitting diodes (OLEDs) continue to attract research interest due to their increasing applications in display and lighting markets. A current field of development is the application of solution-based deposition techniques for the organic layers. However, in general, corresponding devices do not yet reach the performance of OLEDs with vapor deposited materials. In the present work, we have used a model system with a sulfur-containing host material in the emission layer that can either be deposited by thermal evaporation or spin coating. The structure of the corresponding devices was analyzed by a combination of transmission electron microscopy and spatially resolved energy-dispersive x-ray analysis. To investigate the devices’ cross section, wedge shaped, electron transparent lamellas were prepared by focused ion beam milling. Significant differences of the spatial distribution of sulfur have been observed in the devices.