gebhart/patrepeval huggingface dataset:
Abstract 1891: OncogenicMDM2-containing exosomes promote pre-metastatic niche establishment (2019)

Extracellular vesicles (EVs) have an important role in the communication between cells for their ability to transfer proteins and other biologically active molecules. EVs have been also described to have unique functions during pre-metastatic niche establishment and maintenance. MDM2 oncoprotein is the best-characterized cellular antagonist of p53 and its amplification is one of the most known mechanisms for tumor development. MDM2 amplification occurs in more than forty different types of malignancies and in 100% of well-differentiated and dedifferentiated liposarcoma (DDLPS). Retroperitoneal DDLPS is especially concerning given its propensity for multifocal local and distant recurrence, accounting for an overall survival rate of 10% at 10 years. Here, we show the presence of MDM2 in tumor EVs and specifically in both DDLPS cell line-derived EVs and patient-serum EVs. We also show the ability of MDM2-cargo to be transferred to recipient preadipocytes. Furthermore, we also demonstrated that this transfer is able to promote a pre-metastatic niche establishment in the DDLPS tumor microenvironment fat bearing areas, inducing: (I) tumor associated phenotype such as enhanced proliferation and migration, in the recipient preadipocytes, by downregulation of MDM2 downstream pathway; (II) pro-inflammatory phenotype through the release of pro-inflammatory cytokines such as IL-8; (III) release of active metalloproteinases such as MMP2 which is crucial for the invasiveness of many disseminating tumors. In conclusion here we report the presence of MDM2, in the patient circulation; importantly showing that MDM2 triggers the release of active MMP-2, we also propose a possible mechanism for pre-metastatic niche establishment and DDLPS multifocal loco-regional recurrence.Citation Format: Lucia Casadei, Federica Calore, Ameya Deshmukh, Abeba Zewdu, Danielle A. Braggio, Paolo Fadda, Martin Wabitsch, Gonzalo Lopez, Jennifer Leight, Dina Lev, Carlo M. Croce, Raphael E. Pollock. Oncogenic MDM2-containing exosomes promote pre-metastatic niche establishment [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 1891.
Preparation and characteristics of superconducting cuprate thin films: Nd(2-x)Ce(x)CuO4 and substituted Bi-system (1990)

Characteristics of the electron-doped-type Nd(2-x)Ce(x)CuO4 systems and substituted Bi2(Sr,Ln)3Cu2Oy system were systematically studied using the high quality thin-film samples. The Nd(2-x)Ce(x)CuO4 thin films with various Ce concentrations, x, were prepared by RF magnetron sputtering on SrTiO3 heated at around 500 C. After subsequent annealing at 1100 C in air, the films showed the c-axis orientation normal to the substrates. By means of the reducing treatment (annealing in a vacuum), superconductivity was induced for the films with 0.14 is less than or equal to x is less than or equal to 0.18. The superconductivity and transport properties of the films were strongly affected by the reducing treatment. The x = 0.15 film exhibited a sharp superconducting transition with zero resistivity at 22 K, in consistent with the diamagnetic properties. The resistivity of the films was fairly low with metallic characteristics, and the sight of the Hall coefficient was negative in the normal state. On the other hand, the normal-state optical measurements showed that the undoped Nd2CuO4 is a semiconductor with a charge transfer gap of 1.3 eV, and that, when Ce ions were doped, a plasma reflection due to the free-carriers came to be seen with the plasma frequency of 1.07 eV for 0.14 is less than or equal to x is less than or equal to 0.18. Moreover, x ray photoemission study revealed that the Cu valence of the film decreased for 2(+) for x = 0 to 1(+) for x = 0.15. These physical properties are in contrast with those of hole-doped-type cuprate superconductors. Bi2(Sr,Ln)3Cu2Oy thin films were also prepared on MgO substrates heated at 600 to 700 C by similar methods. It was found that the growth conditions for Bi-systems with two CuO2 planes were different for each composition and species of lanthanoid in the films. Moreover, preparation of Bi-system with three CuO2 planes was very difficult when lanthanoid atoms were doped in the system. Their electric transport properties and x ray photoemission spectroscopy were investigated. Carrier concentration and Cu valence were discussed with regard to the superconductivity.
High-dispersive mirrors for femtosecond lasers (2008)

We report on the development of highly dispersive mirrors for chirped-pulse oscillators (CPO) and amplifiers (CPA). In this proof-of-concept study, we demonstrate the usability of highly dispersive multilayer mirrors for high-energy femtosecond oscillators, namely for i) a chirped-pulse Ti:Sa oscillator and ii) an Yb:YAG disk oscillator. In both cases a group delay dispersion (GDD) of the order of 2x10(4) fs(2) was introduced, accompanied with an overall transmission loss as low as approximately 2 per cent. This unprecedented combination of high dispersion and low loss over a sizeable bandwidth with multilayer structures opens the prospects for femtosecond CPA systems equipped with a compact, alignment-insensitive all-mirror compressors providing compensation of GDD as well as higher-order dispersion.
Abstract 4774: LC-MS/MS detection of the dansyl-modified biologically active CDK inhibitor VMY-1-103 using an in vivo model system (2012)

Abstract The development of new small molecule kinase inhibitors as cancer therapeutic drugs has placed increased emphasis on determining the mechanism of action of the new compounds preclinically, such as in genetically engineered mice or using human tissue xenografts. The ability to accurately measure the pharmacokinetics and pharmacodynamics of new compounds is often hampered by the lack of suitable platforms to measure serum and tissue distribution with high sensitivity and specificity. We have recently developed a novel dansylated cyclin dependent kinase inhibitor called VMY-1-103 (VMY) that has purvalanol B as its base compound. We established that VMY was significantly more effective in blocking cell cycle progression and inducing apoptosis than purvalanol B. Since dansylation is frequently used to enhance mass spectrometry-based quantitative analyses, we hypothesized that this modality could similarly be used to evaluate the tissue distribution of VMY in mouse models. In the current study, we developed a rapid liquid phase-extraction of VMY and purvalanol B from mouse tissue and validated a sensitive liquid chromatography-tandem mass spectrometry based assay to quantify tissue delivery of these compounds in vivo. Comparisons of the standard curves for VMY versus Purvalanol B revealed a greater than 7.7-fold increase in sensitivity for VMY. Using both the ND-2 SmoA1, a genetically engineered mouse model of medulloblastoma, and normal mice, we quantified tissue delivery by mass spectrometry as well as Area Under the Curve (AUC) analysis by integrating the peak intensity normalized by an internal standard. Our analyses revealed that VMY delivered intraperitoneally at 20mg/kg was found at its highest concentration in serum 4 hours after injection. We further established that VMY was distributed in multiple tissues, including pancreas and prostate at varying levels and, importantly, we establish that VMY was able to traverse an intact blood-brain barrier as it was detected in the brain and cerebellum of normal mice. Purvalanol B delivered in the same manner as VMY was found at significantly lower levels in all tissues examined and unlike VMY, we failed to detect purvalanol B in brain or and cerebellar tissue. The significance of this study illustrates that a biologically active compound can be modified by dansylation and exhibit both increased sensitivity to mass spectrometry and enhanced bioavailability and tissue delivery, including the brain, in vivo. We also define a simple mass spectrometry-based tissue and serum assay. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 4774. doi:1538-7445.AM2012-4774
The effect of band gap alignment on the hole transport from semiconducting block copolymers to quantum dots (2013)

Semiconducting hole transporting block copolymers were chemically modified to adjust their energy levels to that of CdSe/CdS/CdZnS red quantum dots. Hybrids with optimized energy levels could be used to build strongly improved quantum dot based LEDs (QLEDs).
Synthesis of 2-(9,10-Dihydro-9,10-propanoanthracen-9-yl)-N-methylethanamine via a [4+2] Cycloaddition (2010)

The synthesis of the tetracyclic molecule 2-(9,10-dihydro-9,10-propano-anthracen-9-yl)-N-methylethanamine (2) as a homologue of the antidepressant 1-(9,10-dihydro-9,10-ethanoanthracen-9-yl)-N-methylmethaneamine (1) was described. The key intermediate 9-(prop-2-en-1-yl)-9,10-dihydro-9,10-propanoanthracen-12-one (7) was successfully synthesized via a [4+2] cycloaddition of alpha-bromoacrolein and 9-allyl-anthracene, followed by ring expansion and samarium diiodide deoxygenation.
GATE: software for the analysis and visualization of high-dimensional time series expression data (2009)

GATE is available for download and is free for academic use from http://amp.pharm.mssm.edu/maayan-lab/gate.htm
High-resolution structure of the p53 core domain: implications for binding small-molecule stabilizing compounds (2006)

The p53 transcriptional regulator is the most frequently mutated protein in human cancers and the majority of tumor-derived p53 mutations map to the central DNA-binding core domain, with a subset of these mutations resulting in reduced p53 stability. Here, the 1.55 A crystal structure of the mouse p53 core domain with a molecule of tris(hydroxymethyl)aminomethane (Tris) bound through multiple hydrogen bonds to a region of p53 shown to be important for repair of a subset of tumor-derived p53-stability mutations is reported. Consistent with the hypothesis that Tris binding stabilizes the p53 core domain, equilibrium denaturation experiments are presented that demonstrate that Tris binding increases the thermodynamic stability of the mouse p53 core domain by 3.1 kJ mol(-1) and molecular-dynamic simulations are presented revealing an overall reduction in root-mean-square deviations of the core domain of 0.7 A when Tris is bound. It is also shown that these crystals of the p53 core domain are suitable for the multiple-solvent crystal structure approach to identify other potential binding sites for possible core-domain stabilization compounds. Analysis of the residue-specific temperature factors of the high-resolution core-domain structure, coupled with a comparison with other core-domain structures, also reveals that the L1, H1-S5 and S7-S8 core-domain loops, also shown to mediate various p53 activities, harbor inherent flexibility, suggesting that these regions might be targets for other p53-stabilizing compounds. Together, these studies provide a molecular scaffold for the structure-based design of p53-stabilization compounds for development as possible therapeutic agents.
Emerging liquid crystal waveguide technology for low SWaP active short-wave infrared imagers (2015)

Raytheon’s innovative active short wave infrared (SWIR) imager uses Vescent Photonic’s emerging liquid crystal waveguide (LCWG) technology to continuously steer the illumination laser beam over the imager field of view (FOV). This approach instantly illuminates a very small fraction of the FOV, which significantly reduces the laser power compared to flash illumination. This reduced laser power directly leads to a reduction in the size, weight and power (SWaP) of the laser. The reduction in laser power reduces the input power and thermal rejection, which leads to additional reduction in the SWaP of the power supplies and thermal control. The high-speed steering capability of the LCWG enables the imager’s SWaP reduction. The SWaP reduction is possible using either global or rolling shutter detectors. In both cases, the LCWG steers the laser beam over the entire FOV while the detector is integrating. For a rolling shutter detector, the LCWG synchronizes the steering with the rolling shutter to illuminate only regions currently integrating. Raytheon’s approach enables low SWaP active SWIR imagers without compromising image quality. This paper presents the results of Raytheon’s active SWIR imager demonstration including steering control and synchronization with the detector integration.
Boundary condition effects on the evolution of a train of vortex pairs in still air (2006)

Abstract Effects of boundary conditions on the performance of compact oscillatory momentum and vorticity generators, commonly known as ‘synthetic jet’, ejecting a train of vortex-pairs into still air, were studied experimentally. The different boundary conditions altered the near-device entrainment process of the zero-net-mass-flux actuator. The measurements included hot-wire and Particle Image Velocimetry, cavity pressures and temperatures. When the actuator operates in still air, a quasi-2D vortex pair is generated due to the extreme shear at the edges of the fluid slug ejected during the blowing stage of each excitation cycle. The vorticity flux exiting the slot determines the resulting vortex-pair circulation. The threshold slot exit velocity, for the current configuration and operating conditions, determines if the vortices will be sucked into the actuator’s cavity or be released. Once released, the vortex convection speed approximately scales with the peak velocity at the slot exit. However, the normalised convection velocity increases with the slot Reynolds number. When even a very short extension is attached to one ‘lip’ of the actuator exit, the jet is deflected in the direction opposite the extended lip, due to the restriction on the entrainment process. When long, one ‘lip’ extension is attached, such that the vortex pair is ejected parallel to a plate, the coherence of the vortices improve, their phase speed and magnitude decrease. The effects of high-frequency excitation, ejected perpendicular to a wall into still air, were also investigated. It was found that the presence of the plate does not have a measurable effect on the wall normal excitation, indicating that the majority of the entrainment is taking place from the forward 180° of the actuator exit plane. When the slot is inclined to the surface at a shallow angle of 30 degrees, an unsteady wall jet is formed, transferring momentum along the wall. This is a direct result of the symmetry break, altering the relative magnitudes of the vortex pair.
An Autonomous Palm-Sized Gliding Micro Air Vehicle (2007)

This article has concentrated on the development of a set of core technologies key to the realization of an autonomous 2-g glider. One aspect which was not discussed in detail was the ease of construction and low cost of an individual MicroGlider. Integration is simplified through a number of rapid prototyping techniques and the low costs of most components allow rigorous testing to be done without worry of substantial damage.
ChemInform Abstract: SYNTHESES OF CORRINS. XII. SYNTHESIS OF A TETRADEHYDROCORRINATOPLATINUM(II) BROMIDE (1977)

Abstract Im Zusammenhang mit der Kenntnis der cytostatischen Wirksamkeit von über N‐Atome koordinierten Pt‐Komplexen wird Pt erstmalig in das chromophore System der Tetradehydrocorrine eingebaut.
Effects on Blood Pressure Decrease in Response to PAF of Impatiens textori MIQ. (2003)

A 35% EtOH extract of flowers of Impatiens textori MIQ. showed an inhibitory effect on blood pressure decrease in response to platelet activating factor (PAF) measured with a blood pressure monitoring system. Bioassay-guided fractionation of the 35% EtOH extract (IT) led to isolation of the flavones apigenin (1) and luteolin (3), which significantly inhibited blood pressure decrease in response to PAF. Their compounds and apigenin 7-glucoside (2), chrysoeriol (4), quercetin (5), quercetin 3-glucoside (6), kaempferol (7), kaempferol 3-glucoside (8) and kaempferol 3-rhamnosyldiglucoside (9) were also isolated from the flowers of I. textori for the first time. This study revealed that the flowers of I. textori might be a possible anti-allergy agent.
Energy saving start-up method by combined use of two position sensorless controls (2013)

This paper proposes a start-up method for reducing electrical energy at starting periods. Minimum current startup is achieved by combining two position sensorless control methods that require no harmonic injection. Position sensorless control for low-speed regions utilizes the induced voltage caused by the magnetic saturation of a permanent magnet synchronous motor with conventional block commutation drive. At acceleration, the position sensorless control is changed to the second method based on back-EMF with sinusoidal commutation drive. This paper also addresses when to change the control, as unconsidered switching may result in start-up failure when used with applications that have periodic load properties. A simple yet powerful method to identify the most suitable timing considering load variation is proposed. The proposed methods are validated using a reciprocating compressor with a large periodic load torque.
Effects of Different Hair Washing Methods on the Contents of Lead,Cadmium,Chromium,and Mercury in Human Scalp Hair (2002)

To discover appropriate hair pretreatment for analysis of hair elements through assessing the effects of four common hair washing methods on the contents of lead,cadmium,chromium,and mercury. To compare the contents of lead,cadmium,chromium,and mercury in hair before and after hair being washed. The effects of different hair washing methods on these four elements differ from each other.Both IAEA method and Haiou detergent have little effect on the contents of these four elements,while Na 2EDTA and dilute nitric acid can remove significant amounts of endogenous and exogenous elements.[Conclusions] Both IAEA method and Haiou detergent can be used as hair pretreatment method to wash and clean hair when the extent of the external contamination of human scalp hair can be neglected; When the external contamination is substantially significant,however,the author does not recommend any washing methods for hair element analysis for the purpose of biological monitoring of body burdens,based on the results from this study.Further study on the combination forms of externally polluted elements with hair,however,needs to be conducted.
Multimodal Perfluorocarbon Nanoemulsions for [sup 19]F MRI, Ultrasonography, and Catalysis of MRgFUS-Mediated Drug Delivery (2011)

Perfluorocarbon nanoemulsions can target lipophilic therapeutic agents to solid tumors and simultaneously provide for monitoring nanocarrier biodistribution via ultrasonography and/or 19F MRI. In the first generation of block copolymer stabilized perfluorocarbon nanoemulsions, perfluoropentane (PFP) was used as the droplet forming compound. Although manifesting excellent therapeutic and ultrasound imaging properties, PFP nanoemulsions were unstable at storage, difficult to handle, and underwent droplet‐to‐bubble transition upon injection that was hard to control. To solve the above problems, perfluoro‐15‐crown‐5‐ether (PFCE) was used as a core forming compound in the second generation of block copolymer stabilized perfluorocarbon nanoemulsions. In the present paper, acoustic, imaging, and therapeutic properties of unloaded and paclitaxel (PTX) loaded PFCE nanoemulsions are reported. The size of paclitaxel‐loaded PFCE nanodroplets (300 nm to 500 nm depending on emulsification conditions) favors their passive accumulation in tumor tissue. PFCE nanodroplets manifest both ultrasound and 19F MR contrast properties, which allows the use of multimodal imaging to monitor nanodroplet biodistribution. Ultrasonography and 19F MRI produced consistent results on nanodroplet biodistribution. Sonication with 1‐MHz therapeutic ultrasound triggered reversible droplet‐to‐bubble transition in PFCE nanoemulsions. Microbubbles formed by acoustic vaporization underwent stable cavitation. In a pilot study on ultrasound‐mediated therapy of a large breast cancer tumor, paclitaxel‐loaded PFCE nanoemulsions combined with 1‐MHz ultrasound (MI≥1.75) showed excellent therapeutic properties. Anticipated mechanisms of the observed effects are discussed.
Digital RF processing: toward low-cost reconfigurable radios (2005)

RF circuits for multi-gigahertz frequencies have recently migrated to state-of-the-art low-cost digital CMOS processes. This article visits fundamental techniques recently developed that migrate RF and analog design complexity to the digital domain for a wireless RF transceiver. All-digital phase locked loop and direct RF sampling techniques allow great flexibility in reconfigurable radio design. Digital signal processing concepts are used to help relieve analog design complexity, allowing one to reduce cost and power consumption in a reconfigurable design environment. Software layers are defined to enable these architectures to develop an efficient software-defined radio. The ideas presented have been used to develop two generations of commercial digital RF processors: a single-chip Bluetooth radio and a single-chip GSM radio.
Uracil in DNA and its processing by different DNA glycosylases (2008)

Uracil in DNA may result from incorporation of dUMP during replication and from spontaneous or enzymatic deamination of cytosine, resulting in U:A pairs or U:G mismatches, respectively. Uracil generated by activation-induced cytosine deaminase (AID) in B cells is a normal intermediate in adaptive immunity. Five mammalian uracil-DNA glycosylases have been identified; these are mitochondrial UNG1 and nuclear UNG2, both encoded by the UNG gene, and the nuclear proteins SMUG1, TDG and MBD4. Nuclear UNG2 is apparently the sole contributor to the post-replicative repair of U:A lesions and to the removal of uracil from U:G contexts in immunoglobulin genes as part of somatic hypermutation and class-switch recombination processes in adaptive immunity. All uracil-DNA glycosylases apparently contribute to U:G repair in other cells, but they are likely to have different relative significance in proliferating and non-proliferating cells, and in different phases of the cell cycle. There are also some indications that there may be species differences in the function of the uracil-DNA glycosylases.
Analysis and Control of Redundant Hybrid Master Arm (1999)

Abstract In a teleoperation system, master device is the most important resource which is closely related to work performance of an operator. Thus, We have developed a human arm-like master arm for last few years. The master arm has characteristics of hybrid kinematics, redundant degree of freedom, distributed force refection function, dextrous motion and worn-type, which are as same as human arm characteristics. And the master arm was more improved in the view point of dexterousness, human friendliness and adaptability than the previous version of KIST master arm. In this paper, design concept, analysis and control methodology of the new master arm (KIST master arm), are dealt with.
Comment gouverner une ville en mutation (2005)

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