gebhart/patrepeval huggingface dataset:
Deletion of Cg-emb in Corynebacterianeae Leads to a Novel Truncated Cell Wall Arabinogalactan, whereas Inactivation of Cg-ubiA Results in an Arabinan-deficient Mutant with a Cell Wall Galactan Core (2005)

The cell wall of Mycobacterium tuberculosis has a complex ultrastructure that consists of mycolic acids connected to peptidoglycan via arabinogalactan (AG) and abbreviated as the mAGP complex. The mAGP complex is crucial for the survival and pathogenicity of M. tuberculosis and is the target of several anti-tubercular agents. Apart from sharing a similar mAGP and the availability of the complete genome sequence, Corynebacterium glutamicum has proven useful in the study of orthologous M. tuberculosis genes essential for viability. Here we examined the effects of particular genes involved in AG polymerization by gene deletion in C. glutamicum. The anti-tuberculosis drug ethambutol is thought to target a set of arabinofuranosyltransferases (Emb) that are involved in arabinan polymerization. Deletion of emb in C. glutamicum results in a slow growing mutant with profound morphological changes. Chemical analysis revealed a dramatic reduction of arabinose resulting in a novel truncated AG structure possessing only terminal arabinofuranoside (t-Araf) residues with a corresponding loss of cell wall bound mycolic acids. Treatment of wild-type C. glutamicum with ethambutol and subsequent cell wall analyses resulted in an identical phenotype comparable to the C. glutamicum emb deletion mutant. Additionally, disruption of ubiA in C. glutamicum, the first enzyme involved in the biosynthesis of the sugar donor decaprenol phosphoarabinose (DPA), resulted in a complete loss of cell wall arabinan. Herein, we establish for the first time, (i) that in contrast to M. tuberculosis embA and embB mutants, deletion of C. glutamicum emb leads to a highly truncated AG possessing t-Araf residues, (ii) the exact site of attachment of arabinan chains in AG, and (iii) DPA is the only Araf sugar donor in AG biosynthesis suggesting the presence of a novel enzyme responsible for "priming" the galactan domain for further elaboration by Emb, resulting in the final maturation of the native AG polysaccharide.
Limpio e invisible: las nuevas tecnologías de transporte constituyen un valioso enlace a un futuro limpio y sostenible (2008)

Cuando se desarrolló, hace más de cien años, una tecnología para el transporte de electricidad, las fuentes locales de energías fósiles en áreas remotas fueron sustituidas por energía renovable en forma de centrales hidroeléctricas. En ese momento, el desarrollo del transporte de energía eléctrica respondió a la necesidad de encontrar nuevas fuentes de energía más que a aspectos medioambientales. Pasados cien años, la situación es bastante diferente. El cambio climático combinado con la necesidad de reducir la emisión de gases de efecto invernadero hacen que los aspectos medioambientales se hayan convertido ahora en el principal factor determinante del desarrollo de la tecnología del transporte de energía. Si bien la electricidad (y la calefacción), con sus métodos actuales de producción y distribución, es el principal contribuyente a los gases del efecto invernadero, también representa el mayor potencial para combatir el cambio climático. Los combustibles fósiles siguen produciendo gran parte de la electricidad mundial. Sin embargo, la generación de energía renovable a partir de fuentes hidráulicas, eólicas y solares está aumentando constantemente. Esto, combinado con los avances en la tecnología del transporte de energía en los últimos veinte años, es fundamental para encontrar una solución que no sólo reduzca los niveles de CO2, sino que lo haga de forma rentable. La solución es llevar hasta el consumidor la energía eléctrica renovable, generada mediante energías hidráulica, eólica o solar, empleando las tecnologías de ABB disponibles hoy en día.
High-Throughput Membrane Surface Modification to Control NOM Fouling (2009)

A novel method for synthesis and screening of fouling-resistant membrane surfaces was developed by combining a high-throughput platform (HTP) approach together with photoinduced graft polymerization (PGP)forfacile modification of commercial poly(aryl sulfone) membranes. This method is an inexpensive, fast, simple, reproducible, and scalable approach to identify fouling-resistant surfaces appropriate for a specific feed. In this research, natural organic matter (NOM)-resistant surfaces were synthesized and indentified from a library of 66 monomers. Surfaces were prepared via graft polymerization onto poly(ether sulfone) (PES) membranes and were evaluated using an assay involving NOM adsorption, followed by pressure-driven-filtration. In this work new and previously tested low-fouling surfaces for NOM are identified, and their ability to mitigate NOM and protein (bovine serum albumin)fouling is compared. The best-performing monomers were the zwitterion [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide, and diacetone acrylamide, a neutral monomer containing an amide group. Other excellent surfaces were synthesized from amides, amines, basic monomers, and long-chain poly(ethylene) glycols. Bench-scale studies conducted for selected monomers verified the scalability of HTP-PGP results. The results and the synthesis and screening method presented here offer new opportunities for choosing new membrane chemistries that minimize NOM fouling.
Crystallization and preliminary X-ray crystallographic study of alanyl-tRNA synthetase from the archaeonArchaeoglobus fulgidus (2007)

In order to analyze the alanyl-tRNA synthetase from the archaeon Archaeoglobus fulgidus, the N-terminal fragment lacking the dimerization domain and the C-terminal dimerization-domain fragment were each overexpressed in Escherichia coli, purified and crystallized. A 3.7 A resolution data set was collected for the N-terminal fragment. The crystal belongs to the tetragonal space group P4(1) or P4(3), with unit-cell parameters a = b = 101.15, c = 124.24 A. For the C-terminal fragment, a SeMet MAD data set was collected to 3.2 A resolution. The crystal belongs to the orthorhombic space group P222(1), with unit-cell parameters a = 124.15, b = 131.91, c = 138.68 A.
Cooperative binding of concanavalin a to thymocyte membrane vesicles augments uptake of α‐aminoisobutyrate (1976)

(1) Membrane vesicles from rabbit thymocytes accumulate alpha-aminoisobutyrate in the presence of 0.1 M NaCl. Uptake is 1/2 maximal after about 2 min and reaches a plateau value (61 pmoles/mg protein) after 30 min. (2) Up to 25 mug concanavalin A/ml, binding of the lectin describes a sigmoid curve indicative of a cooperative process. (3) At lectin concentrations up to 8 mug/ml, lectin binding enhances the uptake of alpha-aminoisobutyrate (maximally 30%).
Active learning and subspace clustering for anomaly detection (2011)

Today, anomaly detection is a highly valuable application in the analysis of current huge datasets. Insurance companies, banks and many manufacturing industries need systems to help humans to detect anomalies in their daily information. In general, a
Primary Results on Safety and Efficacy From the LEADLESS II–Phase 2 Worldwide Clinical Trial (2021)

A re-designed single chamber leadless pacemaker (LP; Aveir) was developed with the goal of improving safety and performance of its predecessor (Nanostim). Herein, we present the first-in-human expe...
Monolithic optical link in silicon-on-insulator CMOS technology (2017)

This work presents a monolithic laterally-coupled wide-spectrum (350 nm < λ < 1270 nm) optical link in a silicon-on-insulator CMOS technology. The link consists of a silicon (Si) light-emitting diode (LED) as the optical source and a Si photodiode (PD) as the detector; both realized by vertical abrupt n<sup>+</sup>p junctions, separated by a shallow trench isolation composed of silicon dioxide. Medium trench isolation around the devices along with the buried oxide layer provides galvanic isolation. Optical coupling in both avalanche-mode and forward-mode operation of the LED are analyzed for various designs and bias conditions. From both DC and pulsed transient measurements, it is further shown that heating in the avalanche-mode LED leads to a slow thermal coupling to the PD with time constants in the ms range. An integrated heat sink in the same technology leads to a ∼ 6 times reduction in the change in PD junction temperature per unit electrical power dissipated in the avalanche-mode LED. The analysis paves way for wide-spectrum optical links integrated in smart power technologies.
Label-free detection of DNA using diffracted laser in nanowall array structures (2011)

We developed a new label-free system using nanowall array structures and realized label-free detection of small molecules and DNA. For the achievement of label-free detection, we used the diffracted laser beam, which was produced by the nanowall array structures when the laser was passed through the nanowall array structures perpendicularly. The diffracted signals showed a good linear relationship between the signal intensity and refractive indices, concentrations of molecules, or DNA length.
Inactivation of clathrin heavy chain inhibits synaptic recycling but allows bulk membrane uptake (2008)

Synaptic vesicle reformation depends on clathrin, an abundant protein that polymerizes around newly forming vesicles. However, how clathrin is involved in synaptic recycling in vivo remains unresolved. We test clathrin function during synaptic endocytosis using clathrin heavy chain (chc) mutants combined with chc photoinactivation to circumvent early embryonic lethality associated with chc mutations in multicellular organisms. Acute inactivation of chc at stimulated synapses leads to substantial membrane internalization visualized by live dye uptake and electron microscopy. However, chc-inactivated membrane cannot recycle and participate in vesicle release, resulting in a dramatic defect in neurotransmission maintenance during intense synaptic activity. Furthermore, inactivation of chc in the context of other endocytic mutations results in membrane uptake. Our data not only indicate that chc is critical for synaptic vesicle recycling but they also show that in the absence of the protein, bulk retrieval mediates massive synaptic membrane internalization.
Heterogeneous co-simulation with SDL and SystemC for protocol modeling (2006)

This paper proposes a heterogeneous co-simulation environment using SDL (specification and description language) and SystemC. The medium access control (MAC) protocol of IEEE802.11 wireless LAN (WLAN) is given as an example applied to this co-simulation approach. After functional specification and software-hardware partitioning processes, we designed a sub-part of MAC that requires real-time operations by SystemC, and the other sub-part assumed to be mostly software-executable by SDL. Those two MAC entities are simultaneously simulated on our proposed simulation platform for the purpose of protocol conformance testing and performance evaluation. The paper also presents the basic architecture to enable signal interaction and system timer synchronization between the SDL-designed part and the SystemC-designed part.
Transport of steroids at model biomembrane surfaces and across organic liquid-aqueous phase interfaces [Erratum to document cited in CA111(14):120701m] (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleTransport of steroids at model biomembrane surfaces and across organic liquid-aqueous phase interfaces [Erratum to document cited in CA111(14):120701m]Victoria M. Knepp and Richard H. GuyCite this: J. Phys. Chem. 1990, 94, 6, 2726Publication Date (Print):March 1, 1990Publication History Published online1 May 2002Published inissue 1 March 1990https://pubs.acs.org/doi/10.1021/j100369a090https://doi.org/10.1021/j100369a090research-articleACS PublicationsRequest reuse permissionsArticle Views22Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
A slot-scanned detector operating on gas-solid state imaging principles (2002)

The concepts of a new detector operating on gaseous-solid state electronic principles for slot-scan imaging of ionizing radiation in two dimensions, is presented. The detector geometry, implemented both with the kinestatic and time delay integration (TDI) principles is a promising candidate for a large number of imaging applications. The proposed detector technology may have significant applications in several areas of medical, industrial and aerospace imaging.
Belinostat in Patients With Relapsed or Refractory Peripheral T-Cell Lymphoma: Results of the Pivotal Phase II BELIEF (CLN-19) Study (2015)

Monotherapy with belinostat produced complete and durable responses with manageable toxicity in patients with relapsed or refractory PTCL across the major subtypes, irrespective of number or type of prior therapies. These results have led to US Food and Drug Administration approval of belinostat for this indication.
A real-time cellular system architecture to experiment with UMTS/HSDPA in a laboratory (2009)

Nowadays wireless wide area networks (e.g. cellular networks) are also used to provide access to Internet and its services. However, protocols and applications not specifically designed for wireless links (e.g., TCP, VoIP) often require a deeper analysis when applied to such links. Performance analysis represents a valid method to evaluate such protocols and applications, modeling their behavior and then working on their improvement. An appropriate solution to support performance evaluation of a wireless system and its support to protocols and applications consists in building a close-to-real test environments. This paper presents MUSA, a testbed that allows experimentation of cellular networks in a laboratory. The presented testbed combines emulation components and real equipments in a single experimentation platform, with the flexibility, scalability and repeatability features of an emulated environment and the possibility of testing real equipments, e.g., real mobile terminals or UMTS/HSDPA wireless cards and base stations. The paper describes also the assessment and calibration of the testbed. It presents an extensive comparison with a commercial 3G network and discusses the unique properties of MUSA. Finally, the paper also presents two case studies in order to highlight the benefits of conducting experimental research in an in-lab fully controlled platform rather than in an operator-controlled network.
Tight Lower Bound on Linear Authenticated Encryption. (2002)

We show that any scheme to encrypt m blocks of size n bits while assuring message integrity, that apart from using m + k invocations of random functions (from n bits to n bits) and vn bits of randomness, is linear in (GF 2) , must have k + v at least \\Omega\\Gammaast m).
Activation of different myogenic pathways: myf-5 is induced by the neural tube and MyoD by the dorsal ectoderm in mouse paraxial mesoderm (1996)

Newly formed somites or unsegmented paraxial mesoderm (UPM) have been cultured either in isolation or with adjacent structures to investigate the influence of these tissues on myogenic differentiation in mammals. The extent of differentiation was easily and accurately quantified by counting the number of beta-galactosidase-positive cells, since mesodermal tissues had been isolated from transgenic mice that carry the n-lacZ gene under the transcriptional control of a myosin light chain promoter, restricting expression to striated muscle. The results obtained showed that axial structures are necessary to promote differentiation of paraxial mesoderm, in agreement with previous observations. However, it also appeared that the influence of axial structures could be replaced by dorsolateral tissues, adjacent to the paraxial mesoderm. To elucidate which of these tissues exerts this positive effect, we cultured the paraxial mesoderm with a variety of adjacent structures, either adherent to the mesoderm or recombined in vitro. The results of these experiments indicated that the dorsal ectoderm exerts a positive influence on myogenesis but only if left in physical proximity to it. In contrast, lateral mesoderm delays the positive effect of the ectoderm (and has no effect on its own) suggesting that this tissue produces an inhibitory signal. To investigate whether axial structures and dorsal ectoderm induce myogenesis through common or separate pathways, we dissected the medial half of the unsegmented paraxial mesoderm and cultured it with the adjacent neural tube. We also cultured the lateral half of the unsegmented paraxial mesoderm with adjacent ectoderm. The induction of the myogenic regulatory factors myf-5 and MyoD was monitored by double staining of cultured cells with antibodies against MyoD and beta-galactosidase since the tissues were isolated from mouse embryos that carry n-lacZ targeted to the myf-5 gene, so that myf-5 expressing cells could be easily identified by either histochemical or immunocytochemical staining for beta-galactosidase. After 1 day in culture myogenic cells from the medial half expressed myf-5 but not MyoD, while myogenic cells from the lateral half expressed MyoD but not myf-5. By the next day in vitro, however, most myogenic cells expressed both gene products. These data suggest that the neural tube activates myogenesis in the medial half of paraxial mesoderm through a myf-5-dependent pathway, while the dorsal ectoderm activates myogenesis through a MyoD-dependent pathway. The possible developmental significance of these observations is discussed and a model of myogenic determination in mammals is proposed.
Field-induced water electrolysis switches an oxide semiconductor from an insulator to a metal (2010)

Water is composed of two strong electrochemically active agents, H+ and OH− ions, but has not been used as an active electronic material in oxide semiconductors. In this study, we demonstrate that water-infiltrated nanoporous glass electrically switches an oxide semiconductor from insulator to metal. We fabricated a field-effect transistor structure on an oxide semiconductor, SrTiO3, using water-infiltrated nanoporous glass—amorphous 12CaO·7Al2O3—as the gate insulator. Positive gate voltage, electron accumulation, water electrolysis and electrochemical reduction occur successively on the SrTiO3 surface at room temperature. This leads to the formation of a thin (~3 nm) metal layer with an extremely high electron concentration (1015–1016 cm−2), which exhibits exotic thermoelectric behaviour. The electron activity of water as it infiltrates nanoporous glass may find many useful applications in electronics or in energy storage. Water is composed of the electrochemically active species, H+ and OH−, but has not been used as an active electronic material. In this study, a field-effect transistor is developed that uses water-infiltrated nanoporous glass as the gate insulator; this new application of water may be useful in electronics and energy storage.
Sparsity Constrained Regression For High-Speed Signal Integrity Modeling (2019)

High-speed signal integrity (SI) modeling usually involves modeling non-linear subsystems. Traditional approaches like response surface modeling (RSM), artificial neural networks (ANN) and support vector machine (SVM) suffer from two major issues. Either they don't scale well when the number of dimensions or variables increase in the non-linear model or they are sensitive to the initial training data used to create the models. For example: RSM approach requires large design of experiments (DoE) when the number of variables increase. With ANN, the designer has to spend a lot of time tuning or optimizing the hyperparameters. In this paper, a new approach based on sparsity constrained regression (SCR) is proposed. This approach can scale even when the non-linear problem has large number of variables and it only has one hyper-parameter to tune making it easy to train and test. A test case based on printed circuit board (PCB) stack-up modeling is used in this paper to test the efficiency of SCR approach. The results are compared against traditional RSM and ANN approaches.
Patellofemoral Contact Pressures After Patellar Distalization: A Biomechanical Study (2017)

Care should be taken to prevent excessive distalization of the patella to avoid patella baja and increased patellofemoral contact pressures during early flexion.
All-Optical NRZ-OOK to RZ-QPSK Error-Free Conversion at 10.7 Gsymbol/s Using Parallel SOA-MZI OOK/BPSK Converters in a MZI Configuration (2007)

We have proposed a novel all-optical NRZ-OOK/RZ-QPSK modulation format converter using parallel SOA-MZIs in a MZI configuration. We experimentally demonstrate an error-free operation of the proposed modulation format conversion at 10.7 Gsymbol/s.
Composition of Schedulability Analyses for Real-Time Multiprocessor Systems (2014)

With increasing popularity and deployment of multi-core chips in embedded systems, a number of real-time multiprocessor scheduling algorithms have been proposed along with their schedulability analyses (or tests), which verify temporal correctness under a specific algorithm. Each of these algorithms often comes with several different schedulability tests, especially when it is difficult to find exact schedulability tests for the algorithm. Such tests usually find different task sets deemed schedulable even under the same scheduling algorithm. While these different tests have been compared with each other in terms of schedulability performance, little has been done on how to combine such different tests to improve the overall schedulability of a given scheduling algorithm beyond a simple union of their individual schedulability. Motivated by this, we propose a composition theory for schedulability tests with two new methods. The first method composes task-level timing guarantees derived from different schedulability tests, and the second one derives system-level schedulability results from a single schedulability test. The unified composition theory with these two methods then utilizes existing schedulability tests effectively so as to cover additional schedulable task sets. The proposed composition theory is shown to be applicable to most existing preemptive/non-preemptive scheduling algorithms. We also present three case-studies, demonstrating how and by how much the theory can improve schedulability by composing existing schedulability tests. Our evaluation results also show that the composition theory makes it possible to cover up to 181.7 percent additional schedulable task sets for preemptive fpEDF, preemptive EDF and non-preemptive EDF scheduling algorithms beyond their existing tests.
ChemInform Abstract: Convenient Preparations of t‐Butyl Esters and Ethers from t‐Butanol. (1998)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.