gebhart/patrepeval huggingface dataset:
Identification of potential target genes for RFX4_v3, a transcription factor critical for brain development (2006)

Regulatory factor X4 variant transcript 3 (Rfx4_v3) gene disruption in mice demonstrated that interruption of a single allele (heterozygous, +/-) prevented formation of the subcommissural organ, resulting in congenital hydrocephalus, while interruption of two alleles (homozygous, -/-) caused fatal failure of dorsal midline brain structure formation. To identify potential target genes for RFX4_v3, we used microarray analysis to identify differentially expressed genes in Rfx4_v3-deficient mouse brains at embryonic day 10.5, before gross structural changes were apparent. Of 109 differentially expressed transcripts, 24 were chosen for validation and 22 were confirmed by real-time PCR. Many validated genes encoded critical proteins involved in brain morphogenesis, such as the signaling components in the Wnt, bone morphogenetic protein (BMP) and retinoic acid (RA) pathways. Cx3cl1, a CX3C-type chemokine gene that is highly expressed in brain, was down-regulated in the Rfx4_v3-null mice. Both human and mouse Cx3cl1 proximal promoters contained highly conserved X-boxes, known cis-acting elements for RFX protein binding. Using the Cx3cl1 promoter as an example of a target gene, we demonstrated direct binding of RFX4_v3 to the Cx3cl1 promoter, and trans-acting activity of RFX4_v3 protein to stimulate gene expression. These data suggest that RFX4_v3 may act upstream of critical signaling pathways in the process of brain development.
Synthesis of carboxy‐ and chloro‐terminated poly(propylene)s using Zn(C2H5)2 as chain transfer reagent (1992)

Abstract Propene was polymerized with conventional and Solvay‐type TiCl 3 combined with Al(C 2 H 5 ) 2 Cl using Zn(C 2 H 5 ) 2 as a chain transfer reagent. The produced terminal Zn‐carbon bonds of the polymer were reacted with carbon dioxide and chlorine in the presence of N ‐methylimidazole followed by hydrolysis to obtain terminally carboxylated and chlorinated poly(propylene) in approximately 50% yield.
Chemical Imaging of the Binder‐Dependent Coke Formation in Zeolite‐Based Catalyst Bodies During the Transalkylation of Aromatics (2019)

Abstract The choice of binder material, added to a zeolite‐based catalyst body, can significantly influence the catalyst performance during a reaction, i. e. its deactivation and selectivity. In this work the influence of the binder in catalyst extrudates on the formation of hydrocarbon deposits was explored during the transalkylation of toluene with 1,2,4‐trimethylbenzene (1,2,4‐TMB). Using in situ UV‐vis micro‐spectroscopy and ex situ confocal fluorescence microscopy approach, coke species were revealed to predominantly form on the rim of zeolite crystals within Al 2 O 3 ‐bound extrudates. It was found that this was due to Al migration between the zeolite crystals and the Al 2 O 3 ‐binder creating additional acid sites near the zeolite external surface. In contrast, minimal isomerization of 1,2,4‐TMB in the SiO 2 ‐bound extrudate allowed greater access to the zeolite internal pore network, creating a more homogeneous coke distribution throughout the zeolite crystals.
Area-Selective Deposition of AlOx and Al-Silicate for Fully Self-Aligned Via Integration (2023)

The rush for better-performing electronics, and manufacturing processes that heavily rely on "top-down" patterning techniques, is making the integration of "self-aligned" fabrication methods, such as area-selective deposition (ASD), a critical objective for continued device scaling. The fully self-aligned via (FSAV) scheme is broadly proposed as a "killer application" to determine whether ASD can shift from an R&D process to high-volume manufacturing. Nevertheless, the lack of a suitable low-κ deposition process has prevented the realization of FSAV by dielectric-on-dielectric ASD. This is primarily due to the high temperature and/or strong oxidizers employed during low-κ dielectric deposition and their unsuitability in the presence of organic masks, such as self-assembled monolayers (SAMs), used to prevent material nucleation during ASD. In this work, AlO<i><sub>x</sub></i> and Al-silicate atomic layer deposition (ALD) processes are studied to provide suitable materials for ASD-enabled FSAV. Dimethylaluminum isopropoxide and H<sub>2</sub>O are utilized to deposit the metal oxide, whereas Al-silicate is grown by adding 2,2-dimethoxy-1,6-diaza-2-silacyclooctane (DMDAcO) pulses to the AlO<i><sub>x</sub></i> ALD cycle. The selectivity of such processes is demonstrated on 50 nm Cu/SiO<sub>2</sub> structures, using octadecanethiol-derived SAMs to inhibit material nucleation on the metal lines. Scanning and transmission electron microscopies are employed to assess the quality of the ASD processes and investigate the mechanisms behind defect generation on a nongrowth surface. X-ray photoelectron spectroscopy measurements show the high purity of the AlO<i><sub>x</sub></i> film, whereas DMDAcO-ligand incorporation into the Al-silicate matrix is observed. Planar capacitor structures are used to assess the electrical properties of both ASD films, revealing that the silicate film exhibits a relatively low κ-value (5.3 ± 0.2), with a high acceleration field factor (32.4 ± 1.4) and a dielectric breakdown voltage of 6.0 ± 0.3 V at 100 °C.
Stomach 3D Reconstruction Based on Virtual Chromoendoscopic Image Generation (2020)

Gastric endoscopy is a standard clinical process that enables medical practitioners to diagnose various lesions inside a patient's stomach. If any lesion is found, it is very important to perceive the location of the lesion relative to the global view of the stomach. Our previous research showed that this could be addressed by reconstructing the whole stomach shape from chromoendoscopic images using a structure-from-motion (SfM) pipeline, in which indigo carmine (IC) blue dye sprayed images were used to increase feature matches for SfM by enhancing stomach surface's textures. However, spraying the IC dye to the whole stomach requires additional time, labor, and cost, which is not desirable for patients and practitioners. In this paper, we propose an alternative way to achieve whole stomach 3D reconstruction without the need of the IC dye by generating virtual IC-sprayed (VIC) images based on image-to-image style translation trained on unpaired real no-IC and IC-sprayed images. We have specifically investigated the effect of input and output color channel selection for generating the VIC images and found that translating no-IC green-channel images to IC-sprayed red-channel images gives the best SfM reconstruction result.
Characterization of Fast Relaxation During BTI Stress in Conventional and Advanced CMOS Devices With $\hbox{HfO}_{2}/\hbox{TiN}$ Gate Stacks (2008)

We evaluate the performance of a novel fast characterization methodology for NBTI and PBTI measurements. We show that the use of a programmable PCI card in combination with linear current amplifiers provides the following means: (a) to perform short BTI stresses down to ~ 30 mus; (b) to perform fast sensing with delay times t <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">delay</sub> ~ 30 mus and a voltage resolution of ~ 1 mV; and (c) to use arbitrary programmable stress-and-sense sequences covering many decades in time. We used the fast PCI card-based measurement system for fast NBTI-relaxation measurements in SiON/poly-Si gate stacks, as well as for a systematic study of PBTI relaxation with HfO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> / TiN gate stacks. We show for the first time that the V <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">t</sub> relaxation after PBT stress in nFETS with HfO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> /TiN gate stacks and the V <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">t</sub> relaxation after NBT stress in pFETs with SiON/poly-Si gate stacks exhibit strong similarities: We found the time dependence of both types of relaxation to exhibit to first order a log(t) dependence over seven orders of magnitude in time, suggesting that both phenomena are governed by charge removal by tunneling and that tunneling front-based modeling may be used to quantify the observations.
Low Power Nanoscale Switching of VO2using Carbon Nanotube Heaters (2018)

Vanadium dioxide (VO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> ) is attractive for a variety of applications in optics and electronics, due to its abrupt insulator-metal transition (IMT) with >10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sup> × change in resistance near room temperature. Use of VO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> in devices will require low power switching, with knowledge of the transition mechanism and behaviour down to nanoscale dimensions. To address this challenge, we use metallic carbon nanotubes (CNTs) with diameter ~1 nm [1] to probe nanoscale IMT in VO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> for the first time. We find that a single CNT locally switches the VO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> at less than half the voltage and power otherwise required. Furthermore, to understand the nanoscale IMT we use scanning probe microscopy (SPM) techniques to study devices during operation.
The role of MoS2nano-slabs in the protection of solid cracking catalysts for the total conversion of heavy oils to good quality distillates (2012)

The total conversion of the oil barrel to good quality fuels and distillates has been an overriding goal in the oil refinery industry. Today, it is even more important to improve the effective use of the energy fossil reserves. The actual technologies still produce variable amounts of low quality fractions (e.g. fuel oil, bunker oil) or by-products (e.g. coke). The evolution of the slurry hydrocracking technology can open the way to achieve the objective. This technology was originally developed in Germany in the first half of the last century and reconsidered by several research groups in the past decades. The catalyst used in the slurry process is still constituted by the same materials developed by German scientists in the last century, i.e. bulk, crystalline, nano-sized iron, molybdenum or tungsten sulfide. We have demonstrated now that it is possible to improve the catalytic performances by combining the excellent hydrogenation, hydrodesulfurization and hydrodemetallation properties of dispersed MoS2 catalyst with those of a conventional cracking catalyst. This dual catalyst system demonstrates for the first time the ability of dispersed MoS2 particles to protect the cracking catalyst against rapid decay due to coke accumulation and metal poisoning.
Solid-Phase Synthesis of Libraries Generated from a 4-Phenyl-2-carboxy-piperazine Scaffold (2001)

Strategies for finding novel structures of therapeutical interest are discussed. The rationale for the selection of the two scaffolds N4-(m-aminophenyl)-piperazine-2-carboxylic acid E and N4-(o-aminophenyl)-piperazine-2-carboxylic F is described. The synthesis of the appropriate precursors to scaffold E and F and their use in solid-phase chemistry are described. A 160-member library was produced combining these novel piperazine scaffolds with eight sulfonyl chlorides/acid chlorides and 10 amines. The compound library prepared was analyzed using LC-MS, showing the expected base peak in all wells at an average purity of 82%.
Current trends in nanobiosensor technology (2011)

The development of tools and processes used to fabricate, measure, and image nanoscale objects has lead to a wide range of work devoted to producing sensors that interact with extremely small numbers (or an extremely small concentration) of analyte molecules. These advances are particularly exciting in the context of biosensing, where the demands for low concentration detection and high specificity are great. Nanoscale biosensors, or nanobiosensors, provide researchers with an unprecedented level of sensitivity, often to the single molecule level. The use of biomolecule-functionalized surfaces can dramatically boost the specificity of the detection system, but can also yield reproducibility problems and increased complexity. Several nanobiosensor architectures based on mechanical devices, optical resonators, functionalized nanoparticles, nanowires, nanotubes, and nanofibers have been demonstrated in the lab. As nanobiosensor technology becomes more refined and reliable, it is likely it will eventually make its way from the lab to the clinic, where future lab-on-a-chip devices incorporating an array of nanobiosensors could be used for rapid screening of a wide variety of analytes at low cost using small samples of patient material.
Meat bone meal as fertiliser for barley and oat (2008)

The traditional production of mineral N and P fertilisers is unsustainable due its reliance on fossil fuels in the case of N, and on limited mineral resource stocks in the case of P. The use of alternative or complementary fertilisers that originate from organic waste materials is gaining interest. Organic farms, especially arable organic farms without livestock, need usable sources of plant nutrients. Meat bone meal (MBM), a potential organic fertiliser for agricultural crops, contains considerable amounts of nutrients (on average 8% N, 5% P, 1% K and 10% Ca). In EU countries, Commission regulation (EC) No 181/2006 authorised the use of MBM as an organic fertiliser. In this study, MBM was compared to conventional mineral NPK fertiliser. Two randomised complete block split-plot field experiments were conducted: one with spring barley (Hordeum vulgare) in two years; and another with oat (Avena sativa) for three years, including a fourth year of testing for residual effect. Compared to mineral fertiliser (20% N, 3% P and 9% K), MBM was applied at three N levels: 60, 90 and 120 kg N ha-1. The grain yield of both cereal species supported by MBM, did not differ from the yield obtained with the mineral fertiliser at any N level. At 120 kg N ha-1, the grain yield level with either type was ca. 4500 kg ha-1 of barley and 5000 kg ha-1 of oat, representing fair averages for Finnish conditions. Moreover, MBM and mineral fertilisation showed no differences in quality in terms of 1000-grain weight, test-weight, protein content and protein yield. Since MBM has a low N/P ratio, P was applied in surplus to attain comparable N levels. Therefore MBM fertilisation should be fitted for crop rotation and for meeting environmental requirements.;
An automated flow platform for accurate determination of gas–liquid–solid reaction kinetics (2020)

An automated flow platform based on a tube-in-tube contactor and micro-packed bed reactor is developed to measure the kinetics of gas–liquid–solid hydrogenation reactions.
Synthesis of [H+.cntnd.(1.1.1)]X- cryptates assisted by intramolecular hydrogen bonding (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of [H+.cntnd.(1.1.1)]X- cryptates assisted by intramolecular hydrogen bondingPier Lucio Anelli, Fernando Montanari, and Silvio QuiciCite this: J. Org. Chem. 1985, 50, 19, 3453–3457Publication Date (Print):September 1, 1985Publication History Published online1 May 2002Published inissue 1 September 1985https://pubs.acs.org/doi/10.1021/jo00219a007https://doi.org/10.1021/jo00219a007research-articleACS PublicationsRequest reuse permissionsArticle Views1188Altmetric-Citations19LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Two-Photon Fluorescence Anisotropy Microscopy for Imaging and Direct Measurement of Intracellular Drug Target Engagement (2015)

Small molecule therapeutic drugs must reach their intended cellular targets (pharmacokinetics) and engage them to modulate therapeutic effects (pharmacodynamics). These processes are often difficult to measure in vivo due to their complexities and occurrence within single cells. It has been particularly difficult to directly measure cellular drug target binding. Fluorescence polarization is commonly used in pharmacological screening assays to measure drug-protein or protein-protein interactions. We hypothesized that fluorescence polarization imaging could be adapted and used with fluorescently labeled drugs to measure drug target engagement in vivo. Here we summarize recent results using two photon fluorescence anisotropy microscopy. Our imaging technique offers quantitative pharmacological binding information of diverse molecular interactions at the microscopic level, differentiating between bound and unbound states. Used in combination with other recent advances in the development of novel fluorescently labeled drugs, we expect that the described imaging modality will provide a window into the distribution and efficacy of drugs in real time and in vivo at the cellular and subcellular level.
Dynamics of FREI with/without cool flame interaction (2022)

The interactions between cool flames and flames with repetitive extinction and ignition (FREI) of stoichiometric n-heptane/air mixture were studied using a micro flow reactor with a controlled temperature profile from 373 to 1300 K. Two different flame dynamics with and without cool flames were observed in reactors with inner diameters dinner of 1 and 2 mm. Cool flames and FREI are spatially separated at dinner= 1 mm, whereas interactions between cool flames and FREI are observed at dinner= 2 mm. At dinner= 1 mm, the brightness intensity from cool flames depends on the inlet velocity (uinlet). Approximately above uinlet= 10 cm/s, the brightness intensity from cool flames decreases with increasing inlet velocity, despite a large amount of mixture input. This is because before low temperature ignition occurs under higher inlet velocity conditions, the mixture archives temperature where negative temperature coefficient is dominant. Reaction front propagation speed of FREI decreases monotonically due to heat loss because the extinction points of FREI are located in higher temperatures than the cool flame region. At dinner= 2 mm, the acceleration of the reaction front in the cool flame region is confirmed experimentally, as predicted in our previous two-dimensional numerical simulations. Additionally, the instantaneous reaction front speed after autoignition is analyzed at dinner= 1 mm. The instantaneous reaction front speed decreases as the time from extinction to ignition tex_ig becomes longer because a moderate mixing zone of reactants and products is formed.
High performance and stable Au-free AlGaN/GaN lateral power diode on 200-mm silicon substrate (2016)

status: Published
Transient receptor potential canonical type 3 channels facilitate endothelium-derived hyperpolarization-mediated resistance artery vasodilator activity (2012)

The spatial localization of TRPC3 and associated channels, receptors, and calcium stores are integral for myoendothelial microdomain function. TRPC3 facilitates endothelial SK(Ca) and IK(Ca) activation, as key components of EDH-mediated vasodilator activity and for regulating mesenteric artery tone.
Label-free cytometry - Novel sorting parameters and opportunities (2014)

The inherent properties of cells and micro-organisms is an under-explored source of specificity for particle sorting. In contrast to standard immunolabeling it allows us to perform fractionation without any chemical preparation of the sample. This improves the accessibility and affordability of the technology. Our contribution in the field is to extend the scope of deterministic lateral displacement (DLD) from merely a very powerful size-based sorting scheme so that it is used to sort with respect to several other physical parameters. We have explored parameters such as shape, deformability, dielectric properties, secretion and density with potential applications in bacteriology, parasitology and oncology.
Convolutional Neural Network (2018)

In Chapter 8, we looked at a traditional neural network (NN). One of the limitations of a traditional NN is that it is not translation invariant—that is, a cat image on the upper right-hand corner of an image would be treated differently from an image that has a cat in the center of the image. Convolutional neural networks (CNNs) are used to deal with such issues.
1918 pandemic influenza virus and Streptococcus pneumoniae co‐infection results in activation of coagulation and widespread pulmonary thrombosis in mice and humans (2015)

To study bacterial co-infection following 1918 H1N1 influenza virus infection, mice were inoculated with the 1918 influenza virus, followed by Streptococcus pneumoniae (SP) 72 h later. Co-infected mice exhibited markedly more severe disease, shortened survival time and more severe lung pathology, including widespread thrombi. Transcriptional profiling revealed activation of coagulation only in co-infected mice, consistent with the extensive thrombogenesis observed. Immunohistochemistry showed extensive expression of tissue factor (F3) and prominent deposition of neutrophil elastase on endothelial and epithelial cells in co-infected mice. Lung sections of SP-positive 1918 autopsy cases showed extensive thrombi and prominent staining for F3 in alveolar macrophages, monocytes, neutrophils, endothelial and epithelial cells, in contrast to co-infection-positive 2009 pandemic H1N1 autopsy cases. This study reveals that a distinctive feature of 1918 influenza virus and SP co-infection in mice and humans is extensive expression of tissue factor and activation of the extrinsic coagulation pathway leading to widespread pulmonary thrombosis.