gebhart/patrepeval huggingface dataset:
Evaluation of the Safety and Immunogenicity in Rhesus Monkeys of a Recombinant Malaria Vaccine for Plasmodium vivax with a Synthetic Toll-Like Receptor 4 Agonist Formulated in an Emulsion (2011)

Plasmodium vivax is the major cause of malaria outside sub-Saharan Africa and inflicts debilitating morbidity and consequent economic impacts in developing countries. In order to produce a P. vivax vaccine for global use, we have previously reported the development of a novel chimeric recombinant protein, VMP001, based on the circumsporozoite protein (CSP) of P. vivax. Very few adjuvant formulations are currently available for human use. Our interest is to evaluate second-generation vaccine formulations to identify novel combinations of adjuvants capable of inducing strong, long-lasting immune responses. In this study rhesus monkeys were immunized intramuscularly three times with VMP001 in combination with a stable emulsion (SE) or a synthetic Toll-like receptor 4 (TLR4) agonist (glucopyranosyl lipid A [GLA]) in SE (GLA-SE). Sera and peripheral blood mononuclear cells (PBMCs) were tested for the presence of antigen-specific humoral and cellular responses, respectively. All groups of monkeys generated high titers of anti-P. vivax IgG antibodies, as detected by enzyme-linked immunosorbent assays (ELISAs) and immunofluorescence assays. In addition, all groups generated a cellular immune response characterized by antigen-specific CD4(+) T cells secreting predominantly interleukin-2 (IL-2) and lesser amounts of tumor necrosis factor (TNF). We conclude that the combination of VMP001 and GLA-SE is safe and immunogenic in monkeys and may serve as a potential second-generation vaccine candidate against P. vivax malaria.
A 29/30-GAUGE DUAL-CHANDELIER ILLUMINATION SYSTEM FOR PANORAMIC VIEWING DURING MICROINCISION VITRECTOMY SURGERY (2011)

Sakaguchi, Hirokazu MD; Oshima, Yusuke MD; Nishida, Kohji MD; Awh, Carl C MD Author Information
A Broadband Miniaturized Microwave Dielectric Spectroscopy System Based on Impedance Sensing (2013)

The main purpose of this thesis is to propose a broadband miniaturized spectroscopy system to detect dielectric constant and loss tangent of lossy organic materials at RF/Microwave frequencies. Complex permittivities of lossy liquids are measured using voltage variations across a sensing element embedded in a capacitive divider circuit. The methodology for characterization of complex permittivity of materials is explained in detail.\n\nThe proposed method provides a unique detection algorithm to extract the dielectric constant and loss tangent of an unknown material exposed to the sensor. The complex permittivity of binary organic mixtures have been extracted to an accuracy better than 5% using sample volumes of 200-300 μL within the frequency range of 1-2 GHz. The results obtained from the measurements are compared to the reported values, and their agreements are presented in this work. To the best of the authors’ knowledge, this is the first work presented for broadband miniaturized self-sustained complex permittivity spectroscopy at microwave frequencies.
Self-Assembly of Spider Silk-Fusion Proteins Comprising Enzymatic and Fluorescence Activity (2018)

The recombinant spider silk protein eADF4(C16) was genetically fused either with esterase 2 (EST2) or green fluorescent protein (GFP). The fusions EST-eADF4(C16) and GFP-eADF4(C16) were spectroscopically investigated and showed native structures of EST and GFP. The structural integrity was confirmed by the enzymatic activity of EST and the fluorescence of GFP. The spider silk moiety retained its intrinsically unstructured conformation in solution and the self-assembly into either nanofibrils or nanoparticles could be controlled by the concentration of phosphate. Particles, however, showed significantly lower activity of the EST and GFP domains likely caused by a steric hindrance. However, upon self-assembly of EST-eADF4(C16) and GFP-eADF4(C16) into fibrils the protein activities were retained. In general, the fusion of globular enzymes with the spider silk domain allows the generation of fibrous biomaterials with catalytic or light emitting properties.
Advances in COVID-19 Vaccine (2021)

A COVID-19 vaccine is a vaccine intended to provide acquired immunity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus causing coronavirus disease 2019 (COVID-19). Prior to the COVID-19 pandemic, there was an established body of knowledge about the structure and function of coronaviruses causing diseases like severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS), which enabled accelerated development of various vaccine technologies during early 2020.On 10 January 2020, the SARS-CoV-2 genetic sequence data was shared through GISAID, and by 19 March, the global pharmaceutical industry announced a major commitment to address COVID-19
Speech acts and dialog TTS. (2010)

The approach outlined in this paper aims to provide better expressivity of unit selection TTS for dialog intended applications while retaining the natural sounding voice quality typical of unit selection synthesis. A small set of speech acts were used to annotate a corpus from one female US English speaker. The corpus was composed of speech read primarily from interactive dialogs of various kinds. Global acoustic variables related to prosody were calculated for each speech act in the corpus. A hierarchical cluster analysis performed on the acoustic variables showed clustering that corresponded to general classes of dialog speech acts. The acoustic prosodic variables were used to specify pitch range parameters of a unit selection Speech Act TTS voice. Listening tests indicated large and significant improvement in rated speech quality for the Speech Act system compared to the Standard TTS system built from the same speaker. Index Terms: speech synthesis, dialog, speech acts, prosody 1.
The Correlation Between the Shape of the Quantum Dot Emission Layer and the Device Characteristics by Mixing the Small Molecular Hole Transport Material (2021)

Proceedings of the International Display Workshops Volume 28 (IDW '21),The Correlation Between the Shape of the Quantum Dot Emission Layer and the Device Characteristics by Mixing the Small Molecular Hole Transport Material
Characterization of Tissue Engineered Endothelial Cell Networks in Composite Collagen-Agarose Hydrogels (2020)

Scaffolds constitute an important element in vascularized tissues and are therefore investigated for providing the desired mechanical stability and enabling vasculogenesis and angiogenesis. In this study, supplementation of hydrogels containing either Matrigel<sup>TM</sup> and rat tail collagen I (Matrigel<sup>TM</sup>/rCOL) or human collagen (hCOL) with SeaPlaque<sup>TM</sup> agarose were analyzed with regard to construct thickness and formation and characteristics of endothelial cell (EC) networks compared to constructs without agarose. Additionally, the effect of increased rCOL content in Matrigel<sup>TM</sup>/rCOL constructs was studied. An increase of rCOL content from 1 mg/mL to 3 mg/mL resulted in an increase of construct thickness by approximately 160%. The high rCOL content, however, impaired the formation of an EC network. The supplementation of Matrigel<sup>TM</sup>/rCOL with agarose increased the thickness of the hydrogel construct by approximately 100% while supporting the formation of a stable EC network. The use of hCOL/agarose composite hydrogels led to a slight increase in the thickness of the 3D hydrogel construct and supported the formation of a multi-layered EC network compared to control constructs. Our findings suggest that agarose/collagen-based composite hydrogels are promising candidates for tissue engineering of vascularized constructs as cell viability is maintained and the formation of a stable and multi-layered EC network is supported.
The potential role of xenogeneic antigen‐presenting cells in T‐cell co‐stimulation (1996)

Abstract: CTLA4‐Fc is a chimeric murine construct consisting of the CD28 homologue CTLA4 and the constant portion of the heavy chain of mouse IgG2a, with a potential to suppress cellular xeno‐immune responses. The aim of this study was to determine the degree of binding of CTLA4 to B7 ligands on cells of different species and to use CTLA4‐Fc as a tool for the study of cross‐species CD28‐B7 interactions. As assessed by flow cytometry, CTLA4‐Fc bound to mouse L‐cells and human Epstein Barr virus (EBV) transformed lymphoblastoid cells and concanavalin A (Con A) or LPS‐stimulated peripheral blood mononuclear cells (PBMC) or splenocytes from rat, dog, and pig. CTLA4‐Fc inhibited the proliferation of Con A‐stimulated PBMC or splenocytes from mouse, rat, dog, and pig, in a dose‐dependent fashion with approximately 80% inhibition at a concentration of 10 μg/ml. It did not inhibit the proliferation of Con A‐stimulated human PBMC, although it did inhibit the human versus human, and human versus pig primed mixed lymphocyte culture (MLC) in a dose‐dependent fashion. At submitogenic concentrations, purified human T‐cells did not proliferate after incubation with Con A alone. However, proliferation occurred with the addition of B7 positive L‐cells or pig PBMC, but not B7‐negative OKT4 cells. Furthermore, CTLA4‐Fc inhibited proliferation in a dose‐dependent fashion. CTLA4‐Fc bound to all species tested and resulted in inhibition of Con A‐stimulated proliferation in these species, except for humans. Human T‐cells proliferated in response to co‐stimulation with xenogeneic B7, and this could be inhibited by CTLA4‐Fc, suggesting that xenogeneic B7 was capable of providing a functionally significant co‐stimulatory signal necessary for human T cell activation in vitro.
Un discomycète inoperculé plutôt discret, Pezoloma ciliifera, et remarques sur le genre Pezoloma (2005)

Description et illustration de Pezoloma ciliifera , un petit ascomycète discret mais remarquable poussant dans les milieux humides. Commentaires sur la systématique du genre Pezoloma.
NIMG-75. FEATURES OF LOCAL GRADIENT DISORDER ON MRI THAT DISTINGUISH RADIATION NECROSIS AND TUMOR RECURRENCE POST-RADIOTHERAPY ARE ASSOCIATED WITH ZONAL NECROSIS, VESSEL WALL THICKENING, HYALINIZATION AND DEMYELINATION: A PRELIMINARY STUDY IN BRAIN TUMORS (2016)

Distinguishing radiation necrosis (RN), from tumor recurrence (RT) on routine MRI is a challenging clinical problem in neuro-oncology. We have recently shown success in distinguishing RT and RN on MRI via CoLlAGe, a new computer extracted image feature that captures localized gradient disorder within a lesion. The objective of this study is to understand the histopathologic and morphologic basis of CoLlAGe in distinguishing RT from RN. 3T MRI studies (Gd-T1w, T2w, FLAIR) and surgical resection specimens were obtained from 34 brain tumor patients: 20 primary (10 RN, 10 RT) and 14 (5 RN, 9 RT) metastatic studies, at least 9-months post-radiation. Histological traits including vessel-wall thickening, demyelination, vascular hyalinization and zonal necrosis were graded by an expert neuropathologist. A ‘score’ between 0 and 4 was assigned based on the percentage of each of the observed attributes on the surgical specimens. Necrotic, enhancing tumor and peritumoral regions were independently delineated by a neuroradiologist on the 3 MRI sequences. 13 CoLlAGe features (e.g. entropy, variance, correlation) were computed for every region for each protocol. A pair-wise Spearman correlation was performed between each feature and the pathologic ‘score’. For primary neoplasms, CoLlAGe features from enhancing Gd-T1w were significantly (p<0.05) associated with zonal necrosis and vessel wall thickening. CoLlAGe features on FLAIR hyperintensities were associated with demyelination. For metastatic cases, vascular hyalinization was significantly associated with 9 of the 13 CoLlAGe features from peritumoral FLAIR regions. Imaging correlates on routine MRI were identified for histological attributes (zonal necrosis, vessel wall thickening, vascular hyalinization and demyelination) observed in RN and RT on surgically resected specimens. These histological attributes contribute to lesion heterogeneity, and may be reflected in the CoLlAGe features at the radiologic scale.
Scalable Real-time Transport of Baseband Traffic (2017)

In wireless deployments, such as Massive-MIMO, where radio front-ends and back-end processing are connected through a transport network, meeting the real-time processing requirements is essential to realize the capacity gains from network scaling. While simple forms of baseband transport have been implemented, their real-time analysis at much larger scale is lacking. Towards this, we present the design, delay, and capacity analysis of baseband transport networks, utilizing results from real-time systems in the context of wireless processing. We propose a novel Fat-Tree-based design, called DISTRO, for baseband transport, which is a real-time network that bounds the maximum end-to-end transport delay of each baseband packet. It achieves this by placing design constraints and bounding the queuing delay at each aggregation point in the network. We further characterize the wireless capacity using DISTRO and provide an efficient search algorithm for the design of a capacity achieving baseband transport.
Photoreconfigurable Supramolecular Nanotube (2013)

A photoreconfigurable bionanotube was developed by Mg(2+)-induced supramolecular polymerization using GroELSP, a mutant barrel-shaped chaperonin protein bearing multiple photochromic spiropyran (SP) units at its apical domains. Upon exposure to UV light, the nonionic SP units isomerize into ionic merocyanine (MC) to afford GroELMC, which is capable of polymerizing with MgCl2. The resultant nanotube (NT) is stable as a result of multiple MC···Mg(2+)···MC bridges but readily breaks up into short NTs, including monomeric GroELSP, by the reverse (MC → SP) isomerization mediated by visible light. When this scission mixture is exposed to UV light, long NTs are reconfigured. A Förster resonance energy transfer (FRET) study revealed that NTs in the dark maintain their sequential integrity. However, when exposed to visible and UV light successively, the NTs lose their sequential memory as a result of intertubular reshuffling of the constituent GroELMC units.
Optimized proteomic analysis of a mouse model of cerebellar dysfunction using amine‐specific isobaric tags (2006)

Recent proteomic applications have demonstrated their potential for revealing the molecular mechanisms underlying neurodegeneration. The present study quantifies cerebellar protein changes in mice that are deficient in plasma membrane calcium ATPase 2 (PMCA2), an essential neuronal pump that extrudes calcium from cells and is abundantly expressed in Purkinje neurons. PMCA2-null mice display motor dyscoordination and unsteady gait deficits observed in neurological diseases such as multiple sclerosis and ataxia. We optimized an amine-specific isobaric tags (iTRAQ)-based shotgun proteomics workflow for this study. This workflow took consideration of analytical variance as a function of ion signal intensity and employed biological repeats to aid noise reduction. Even with stringent protein identification criteria, we could reliably quantify nearly 1000 proteins, including many neuronal proteins that are important for synaptic function. We identified 21 proteins that were differentially expressed in PMCA2-null mice. These proteins are involved in calcium homeostasis, cell structure and chromosome organization. Our findings shed light on the molecular changes that underlie the neurological deficits observed in PMCA2-null mice. The optimized workflow presented here will be valuable for others who plan to implement the iTRAQ method.
0.5-V high-speed circuit designs for nanoscale SoCs &#x2014; Challenges and solutions (2011)

Some solutions are proposed and evaluated by simulation after the challenges facing the creation of 0.5-V nanoscale SoCs are clarified. First, the repair techniques and nanoscale FD-MOSFETs are discussed in terms of their V <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">t</sub> -variation. Second, 0.5-V dual-V <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">DD</sub> dual-V <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">t</sub> logic circuits with gate-source reverse-biasing schemes are proposed. Third, a boosted word-voltage six-transistor (6-T) SRAM cell is evaluated with a 25-nm planar FD-SOI MOSFET and then a FinFET, revealing that the FinFET drastically improves the voltage margin and speed of the 6-T cell. Finally, the feasibility of a 0.5-V 25-nm SoC comprising a 1-Gb SRAM and 160-Mgate logic block is studied. We conclude that an SoC like this with a competitive speed while reducing the power to about one-tenth that of a conventional 1-V 32-nm CMOS LSI is possible, if the above-described devices and circuits are used and the within-wafer V <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">t</sub> -variations are stringently controlled and/or compensated for.
Disruption of DDX53 coding sequence has limited impact on iPSC-derived human NGN2 neurons (2023)

DDX53-3TC mutation does not alter NGN2 neuronal function in these experiments, suggesting that synaptic deficits causing ASD are unlikely in this cell type.
Catalytic Cyclopropanation by Myoglobin Reconstituted with Iron Porphycene: Acceleration of Catalysis due to Rapid Formation of the Carbene Species (2017)

Myoglobin reconstituted with iron porphycene catalyzes the cyclopropanation of styrene with ethyl diazoacetate. Compared to native myoglobin, the reconstituted protein significantly accelerates the catalytic reaction and the k<sub>cat</sub>/K<sub>m</sub> value is 26-fold enhanced. Mechanistic studies indicate that the reaction of the reconstituted protein with ethyl diazoacetate is 615-fold faster than that of native myoglobin. The metallocarbene species reacts with styrene with the apparent second-order kinetic constant of 28 mM<sup>-1</sup> s<sup>-1</sup> at 25 °C. Complementary theoretical studies support efficient carbene formation by the reconstituted protein that results from the strong ligand field of the porphycene and fewer intersystem crossing steps relative to the native protein. From these findings, the substitution of the cofactor with an appropriate metal complex serves as an effective way to generate a new biocatalyst.
Surface Energy‐Mediated Self‐Catalyzed CsPbBr3 Nanowires for Phototransistors (Adv. Electron. Mater. 12/2022) (2022)

Phototransistor Devices Phototransistor devices based on individual single cubic phase CsPbBr3 perovskite nanowires are fabricated and thoroughly studied. The unique charge transport pathways along the axial direction hold great potential in information technology. More details can be found in article number 2200727 by Johnny C. Ho and co-workers.
Immunogenicity and safety of an inactivated hepatitis a vaccine in anti‐HIV positive and negative homosexual men (1995)

The immunogenicity, reactogenicity, and safety of an inactivated hepatitis A vaccine were assessed in anti-HIV positive homosexual men. Fourteen anti-HIV positive (group 1) and 20 anti-HIV negative (group 2) men received vaccine (containing 720 ELISA units of hepatitis A antigen per dose) intramuscularly at 0, 1, and 6 months. Twelve unvaccinated anti-HIV positive men (group 3) were included as controls to evaluate disease progression. Seroconversion (anti-hepatitis V virus (HAV) > or = 20 mlU/ml) was higher in group 2 than group 1 at months 2 (100% vs. 73%) and 7 (100% vs. 77%). Group 2 had higher antibody titres than group 1 at months 1 (201 vs. 92 mlU/ml) and 7 (1,687 vs. 636 mlU/ml). The decline in CD4+ cells between months 0 and 7 was similar in groups 1 and 3 (6.4% vs. 16.2%), showing no evidence for harmful effect of the vaccine on the course of HIV infection. This hepatitis A vaccine appears safe, well tolerated, but less immunogenic in HIV positive homosexual men.
Eteplirsen treatment for Duchenne muscular dystrophy (2018)

This study provides Class II evidence of the muscle cell penetration, exon skipping, and induction of novel dystrophin expression by eteplirsen, as confirmed by 4 assays.
11 Copeptin improves early risk stratification by Grace Score in non ST-elevation myocardial infarction; NT-proBNP does not: Abstract 11 Figure 1 (2011)

Background Risk stratification is vital to the optimal management of patients with non ST-elevation myocardial infarction (NSTEMI) however, current tools are not fully discriminatory. Copeptin, the stable 39 amino acid C-terminal portion of pro-vasopressin, is a recognised prognostic marker in ST elevation myocardial infarction (STEMI) that is also useful to exclude MI as levels rise early after onset. Copeptin has not been evaluated in a NSTEMI population to date. Aims We hypothesised that copeptin is an independent predictor of mortality following NSTEMI and, in accordance with AHA criteria for the evaluation of novel biomarkers, assess whether copeptin adds prognostic information to GRACE risk score (GRACE-RS). We use NT-proBNP for comparison. Methods and Results In this prospective observational study plasma copeptin and NT-proBNP was measured in 754 NSTEMI patients (519 men, median age 70±13 years) within 36 h of symptoms. The primary endpoint of all-cause mortality at 6 months was reached by 56 (7.4%) patients. Median copeptin levels were 7.9 range 0.3 to 523.0 pmol/l and were significantly higher in those that reached the primary endpoint than the event free survivors; median (IQR), 32.0 (12.0–88.7) vs 7.2 (4.0–16.7) respectively p&lt;0.001. Both copeptin and NT-proBNP were predictive of the primary endpoint on univariate Cox regression analysis (HR 5.98 p&lt;0.0005 and HR 6.07 p&lt;0.0005 respectively). On adjustment for baseline clinical and biochemical variables copeptin remained predictive (HR 3.03 p=0.009) but NT-proBNP did not (p=0.70). Kaplan-Meier analysis revealed that supra-median levels of copeptin were associated with increased mortality (log rank 28.4 p&lt;0.001). ROC curve c-statistics for GRACE-RS of 0.799 increased to 0.835 when combined with copeptin (0.785), when combined with NT-proBNP (0.730) increased to 0.802. Re-classification analysis shows that copeptin improves accuracy of risk stratification when combined with the GRACE-RS as determined by net reclassification improvement (NRI 13.3% p=0.008) whereas, NT-proBNP does not (NRI −4.9% p=0.21). The relative utilities for logistic regression models using GRACE-RS alone, GRACE-RS + copeptin and GRACE-RS + NTproBNP as covariates are shown in Abstract 11 figure 1. The relevant region was the region to the right of the sample risk for 6 months mortality, 0.074. The relative utility for GRACE-RS + copeptin was consistently more than the relative utility for GRACE-RS + NTproBNP across a range of risks; for example at a risk threshold of 15% the additional utility of adding copeptin to the GRACE-RS was 0.097 compared to 0.009 for NTproBNP. Abstract 11 Figure 1 Conclusions High plasma copeptin levels indicate a worse outcome in NSTEMI patients. We have demonstrated that copeptin fulfils AHA criteria by improving risk stratification over established markers GRACE score and NT-proBNP. Copeptin is also useful for rapid rule-out of MI and the current findings further support clinical uptake.