gebhart/patrepeval huggingface dataset:
Extraction of Microfibrils from Bacterial Cellulose Networks for Electrospinning of Anisotropic Biohybrid Fiber Yarns (2010)

Electrospinning of uniform biohybrid fibers with concealed cellulose microfibrils (CMF) is reported as a promising and environmentally sound concept for reinforcement of polymer nonwoven fiber systems of fine dimensions. The extraction and refinement of the high-strength crystalline microfibril bundles (15−20 nm thick) from bacterial cellulose networks is presented, as well as their morphology prior to and post electrospinning, Nanofibers composed of a poly(methyl methacrylate) (PMMA) matrix with cellulose contents reaching 20 wt % were repeatedly obtained. A high degree of dispersion of the microfibrils was obtained for a variety of CMF contents and the aggregation of the CMF was greatly suppressed as the microfibrils were aligned and rapidly sealed inside the acrylate matrix during the continuous formation of the fibers. The limited CMF aggregation up to 7 wt % was related to a suppressed phase separation caused by the rapid solidification of the polymer solutions during spinning. The fibers’ diameters decreased significantly from ∼1.8 μm (1 wt %) to ∼100 nm (20 wt %) with increasing cellulose contents, resulting in CMF agglomerations and percolating architectures within the acrylate host, which was consistent with microscopy, thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) evaluations. The nominal content of cellulose in the fibers was assessed by Lorentzian profile fit assignment of the crystalline vs amorphous fractions of the fibers’ X-ray diffractograms. TGA of fibers with low CMF content revealed that both CMF and PMMA showed a significantly improved thermal stability in the composite material. The biohybrid fibers were continuously aligned into an anisotropic nanocomposite yarns from a liquid support during spinning. The strategy described herein may allow for new mechanically robust nonwoven fiber systems, or be used as implemented on existing electrospun formulations that are lacking mechanical integrity. It is envisioned that the cellulose microfibrils may be of importance in biomedical applications where biocompatibility is a requirement.
Tau imaging detects distinctive distribution of tau pathology in ALS/PDC on the Kii Peninsula (2018)

[<sup>11</sup>C]PBB3 PET visualized the characteristic topography of tau pathology in Kii ALS/PDC, corresponding to clinical phenotypes of this disease.
Retroviral Transduction of Hematopoietic Progenitors Derived From Human Embryonic Stem Cells (2006)

It has been recently identified that cytokines and BMP-4 promote hematopoiesis from human embryonic stem cells (hESC) and that, before hematopoietic commitment, a rare subpopulation of cells lacking CD45, but expressing PECAM-1, Flk-1, and VE-cadherin (hereinafter termed CD45(neg)PFV precursors), are exclusively responsible for hematopoietic cell fate on cytokine stimulation. Efficient strategies to stably transduce these hematopoietic precursors specifically generated from hESCs would provide a novel and desirable tool to study hematopoietic development through the introduction and characterization of candidate genes suspected to regulate self-renewal processes of hESC-derived hematopoietic cells or dynamically track hESC-derived hematopoietic stem cells in vivo. To date, only transient transfection and stable transduction using lentiviral vectors have been reported in undifferentiated hESC followed by random and spontaneous differentiation into different cell types. However, protocols for stable transduction of hematopoietic progenitors prospectively derived from hESC need to be developed yet. In the present chapter, we described detailed methods on the recently characterized and optimized GALV-pseudotyped retroviral gene transfer strategy to stably transduce the hematopoietic progenitor cells prospectively derived from CD45(neg)PFV hemogenic precursors as a vital tool to study hematopoietic development and to characterize candidate genes suspected to eventually confer robust and sustained repopulating ability to hESC-derived hematopoietic cells.
Crystal structures of Phanerochaete chrysosporium pyranose 2‐oxidase suggest that the N‐terminus acts as a propeptide that assists in homotetramer assembly (2013)

The flavin-dependent homotetrameric enzyme pyranose 2-oxidase (P2O) is found mostly, but not exclusively, in lignocellulose-degrading fungi where it catalyzes the oxidation of β-d-glucose to the corresponding 2-keto sugar concomitantly with hydrogen peroxide formation during lignin solubilization. Here, we present crystal structures of P2O from the efficient lignocellulolytic basidiomycete Phanerochaete chrysosporium. Structures were determined of wild-type PcP2O from the natural fungal source, and two variants of recombinant full-length PcP2O, both in complex with the slow substrate 3-deoxy-3-fluoro-β-d-glucose. The active sites in PcP2O and P2O from Trametes multicolor (TmP2O) are highly conserved with identical substrate binding. Our structural analysis suggests that the 17 °C higher melting temperature of PcP2O compared to TmP2O is due to an increased number of intersubunit salt bridges. The structure of recombinant PcP2O expressed with its natural N-terminal sequence, including a proposed propeptide segment, reveals that the first five residues of the propeptide intercalate at the interface between A and B subunits to form stabilizing, mainly hydrophobic, interactions. In the structure of mature PcP2O purified from the natural source, the propeptide segment in subunit A has been replaced by a nearby loop in the B subunit. We propose that the propeptide in subunit A stabilizes the A/B interface of essential dimers in the homotetramer and that, upon maturation, it is replaced by the loop in the B subunit to form the mature subunit interface. This would imply that the propeptide segment of PcP2O acts as an intramolecular chaperone for oligomerization at the A/B interface of the essential dimer.
Lagrangian Analysis and Predictability of Coastal and Ocean Dynamics 2000 (2002)

The first Lagrangian Analysis and Predictability of Coastal and Ocean Dynamics (LAPCOD) meeting took place in Ischia, Italy, 2-6 October 2000. The material presented at LAPCOD 2000 indicated both a maturing of Lagrangian-based observing systems and the development of new analysis and assimilation techniques for Lagrangian data. This summary presents a review of the state-of-the-art technology in Lagrangian exploration of oceanic and coastal waters that was presented at the meeting.
Optimization of fiber Bragg grating parameters for sensing applications (2017)

Fiber Bragg Gratings (FBGs) are increasingly being employed in a novel range of applications, especially in sensing and measurement field. Some of these novel FBG-based sensing applications, especially those requiring high resolution sensing in harsh environments, impose challenges on Bragg gratings and their performance. Additionally, there is a growing list of Fiber Bragg Grating types and manufacturing techniques, each with its own strengths and disadvantages. With the new generation of fiber optic interrogation technologies reaching femtometer-level resolution in Bragg wavelength tracking, the achievable accuracy and stability of the sensing system is becoming limited by the performance of the employed Bragg grating itself. In many cases, correct selection and definition of the FBG parameters can result in defining the success of the sensing system. Here, we explore the specifications of Bragg gratings that are most relevant to FBG-based sensors, propose their characterization and analysis methodologies and explore their effects for both static and dynamic sensing applications in combination with tunable laser based fiber optic interrogation techniques. Bragg gratings manufactured by several different techniques are compared to demonstrate their suitability for different types of sensing applications. Several application focused examples are also provided to demonstrate the importance of the parameters for detection of strain, pressure, sound, vibration and tilt using fiber optic sensors.
Microscaled and nanoscaled platinum sensors (2010)

We show small and robust platinum resistive heaters and thermometers that are defined by microlithography on silicon substrates. These devices can be used for a wide range of applications, including thermal sensor arrays, programmable thermal sources, and even incandescent light emitters. To explore the miniaturization of such devices, we have developed microscaled and nanoscaled platinum resistor arrays with wire widths as small as 75 nm, fabricated lithographically to provide highly localized heating and accurate resistance (and hence temperature) measurements. We present some of these potential applications of microfabricated platinum resistors in sensing and spectroscopy.
nti glucocorticoid receptor transcripts lack sequences encoding the amino-terminal transcriptional modulatory domain. (1990)

Glucocorticoid induction of cell death (apoptosis) in mouse lymphoma S49 cells has long been studied as a molecular genetic model of steroid hormone action. To better understand the transcriptional control of glucocorticoid-induced S49 cell death, we isolated and characterized glucocorticoid receptor (GR) cDNA from two steroid-resistant nti S49 mutant cell lines (S49.55R and S49.143R) and the wild-type parental line (S49.A2). Our data reveal that nti GR transcripts encode intact steroid- and DNA-binding domains but lack 404 amino-terminal residues as a result of aberrant RNA splicing between exons 1 and 3. Results from transient cotransfection experiments into CV1 cells using nti receptor expression plasmids and a glucocorticoid-responsive reporter gene demonstrated that the truncated nti receptor exhibits a reduced transcriptional regulatory activity. Gene fusions containing portions of both the wild-type and the nti GR-coding sequences were constructed and used to functionally map the nti receptor mutation. We found that the loss of the modulatory domain alone is sufficient to cause the observed defect in nti transcriptional transactivation. These results support the proposal that glucocorticoid-induced S49 cell death requires GR sequences which have previously been shown to be required for transcriptional regulation, suggesting that steroid-regulated apoptosis is controlled at the level of gene expression.
Activating and Elucidating Metabolism of Complex Sugars in Yarrowia lipolytica (2015)

The oleaginous yeast Yarrowia lipolytica is an industrially important host for production of organic acids, oleochemicals, lipids, and proteins with broad biotechnological applications. Albeit known for decades, the unique native metabolism of Y. lipolytica for using complex fermentable sugars, which are abundant in lignocellulosic biomass, is poorly understood. In this study, we activated and elucidated the native sugar metabolism in Y. lipolytica for cell growth on xylose and cellobiose as well as their mixtures with glucose through comprehensive metabolic and transcriptomic analyses. We identified 7 putative glucose-specific transporters, 16 putative xylose-specific transporters, and 4 putative cellobiose-specific transporters that are transcriptionally upregulated for growth on respective single sugars. Y. lipolytica is capable of using xylose as a carbon source, but xylose dehydrogenase is the key bottleneck of xylose assimilation and is transcriptionally repressed by glucose. Y. lipolytica has a set of 5 extracellular and 6 intracellular β-glucosidases and is capable of assimilating cellobiose via extra- and intracellular mechanisms, the latter being dominant for growth on cellobiose as a sole carbon source. Strikingly, Y. lipolytica exhibited enhanced sugar utilization for growth in mixed sugars, with strong carbon catabolite activation for growth on the mixture of xylose and cellobiose and with mild carbon catabolite repression of glucose on xylose and cellobiose. The results of this study shed light on fundamental understanding of the complex native sugar metabolism of Y. lipolytica and will help guide inverse metabolic engineering of Y. lipolytica for enhanced conversion of biomass-derived fermentable sugars to chemicals and fuels.
A Scheduling and Rescheduling Decision Support System for Apparel Manufacturing (2021)

The manufacturing environment for apparel is subject to a variety of constraints, stochasticity, and unforeseen events. In order to create an accurate scheduling-system for this environment, these complexities must be considered. This article presents the development and the application of a scheduling and rescheduling decision support system for an apparel manufacturer. Furthermore, the results of applying the proposed system are presented and discussed. The scheduling and rescheduling decision support system presented in this article takes advantage of a variable neighborhood search and Monte Carlo simulation in order to minimize tardiness in the presence of different release times, sequence-based setup times, blocking, and resource constraints. The results show that the quality of the schedules generated by the proposed scheduling and rescheduling decision support system is superior to the current firm’s scheduling practice, which is based on an earliest due date heuristic. Moreover, the percentage of the realized schedule and overall equipment effectiveness were improved.
Global Distribution of the Reniform Nematode Genus Rotylenchulus with the Synonymy of Rotylenchulus macrosoma with Rotylenchulus borealis (2020)

Reniform nematodes of the genus <i>Rotylenchulus</i> are semi-endoparasites of numerous herbaceous and woody plant roots that occur largely in regions with temperate, subtropical, and tropical climates. In this study, we compared 12 populations of <i>Rotylenchulus</i><i>borealis</i> and 16 populations of <i>Rotylenchulus</i><i>macrosoma</i>, including paratypes deposited in nematode collections, confirming that morphological characters between both nematode species do not support their separation. In addition, analysis of molecular markers using nuclear ribosomal DNA (<i>28S</i>, <i>ITS1</i>) and mitochondrial DNA (<i>coxI</i>) genes, as well as phylogenetic approaches, confirmed the synonymy of <i>R. macrosoma</i> with <i>R. borealis</i>. This study also demonstrated that <i>R. borealis</i> (= <i>macrosoma</i>) from Israel has two distinct rRNA gene types in the genome, specifically the two types of <i>D2-D3</i> (A and B). We provide a global geographical distribution of the genus <i>Rotylenchulus</i>. The two major pathogenic species (<i>Rotylenchulus</i><i>reniformis</i> and <i>Rotylenchulus</i><i>parvus</i>) showed their close relationship with warmer areas with high annual mean temperature, maximum temperature of the warmest month, and minimum temperature of the coldest month. The present study confirms the extraordinary morphological and molecular diversity of <i>R. borealis</i> in Europe, Africa, and the Middle East and comprises a paradigmatic example of remarkable flexibility of ecological requirements within reniform nematodes.
Examining Cross Frequency Interference Effects in Multi-Frequency GNSS Receivers (2023)

Radiofrequency interference (RFI) can have many effects on GNSS receivers. One effect that is less commonly discussed is how RFI on one GNSS frequency band may affect a separate frequency band due to the design of its user equipment (antenna, receiver). It is useful to examine these effects to better understand actual receiver vulnerabilities. It helps with developing specifications and guidelines to limit such vulnerabilities. Furthermore, understanding and limiting these effects can help more effectively develop receiver based detect spoofing and jamming. This paper examines cross frequency effects through empirical testing of several types of GNSS antennas and receivers. Equipment ranging from consumer off the shelf (COTS) to high end survey grade were tested in an anechoic chamber with reradiated GNSS and interference signals. The system response is analyzed by looking at spectrum, automatic gain control (AGC), and carrier to noise ratio (C/No) results on multiple frequency band when there is RFI on one frequency band. The antennas tested showed different behavior with the same RFI across frequency bands. One antenna had little cross frequency effects while the other showed some noticeable effects on one band from interference on another. Different receivers also showed different responses across frequency bands with the same RFI. For example, we tested three models of dual frequency GNSS smartphone tested. One model could not utilize L5 when there was interference on L1 while other smartphones did not show this effect. This paper highlights different cross frequency effects that occur with RFI with different empirical tests. It demonstrates the importance of understanding implementation and actual performance and not just assuming each frequency band will operate independently.
ANYL 403-Microfluidic immunoaffinity capture cassette for rapid isolation and processing of neutrophils from whole blood (2007)

ANYL 403-Microfluidic immunoaffinity capture cassette for rapid isolation and processing of neutrophils from whole blood
Computer vision in 3D interactivity (panel) (1998)

Article Computer vision in 3D interactivity (panel) Share on Authors: Mark Holler Intel Corp. Intel Corp.View Profile , Ingrid Carlbom Bell Labs Bell LabsView Profile , Steven Feiner Columbia Univ., New York, NY Columbia Univ., New York, NYView Profile , George Robertson Microsoft Research Microsoft ResearchView Profile , Demetri Terzopoulos Intel Corp; and Univ. of Toronto, Toronto, Ont., Canada Intel Corp; and Univ. of Toronto, Toronto, Ont., CanadaView Profile Authors Info & Claims SIGGRAPH '98: ACM SIGGRAPH 98 Conference abstracts and applicationsJuly 1998 Pages 220–222https://doi.org/10.1145/280953.281598Published:21 July 1998 0citation136DownloadsMetricsTotal Citations0Total Downloads136Last 12 Months3Last 6 weeks0 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
Conformance-While-Drilling Technology Proposed to Optimize Drilling and Production (1999)

Conformance-While-Drilling Technology Proposed to Optimize Drilling and Production Ron Sweatman; Ron Sweatman Halliburton Energy Services Inc. Search for other works by this author on: This Site Google Scholar James Heathman; James Heathman Halliburton Energy Services Inc. Search for other works by this author on: This Site Google Scholar Ronnie Faul; Ronnie Faul Halliburton Energy Services Inc. Search for other works by this author on: This Site Google Scholar Naz Gazi Naz Gazi Halliburton Energy Services Inc. Search for other works by this author on: This Site Google Scholar Paper presented at the Middle East Oil Show and Conference, Bahrain, February 1999. Paper Number: SPE-53312-MS https://doi.org/10.2118/53312-MS Published: February 20 1999 Cite View This Citation Add to Citation Manager Share Icon Share Twitter LinkedIn Get Permissions Search Site Citation Sweatman, Ron, Heathman, James, Faul, Ronnie, and Naz Gazi. "Conformance-While-Drilling Technology Proposed to Optimize Drilling and Production." Paper presented at the Middle East Oil Show and Conference, Bahrain, February 1999. doi: https://doi.org/10.2118/53312-MS Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex Search nav search search input Search input auto suggest search filter All ContentAll ProceedingsSociety of Petroleum Engineers (SPE)SPE Middle East Oil and Gas Show and Conference Search Advanced Search This paper was prepared for presentation at the 1999 SPE Middle East Oil Show held in Bahrain, 20-23 February 1999. Keywords: placement, well control, drilling fluid management & disposal, circulation, cwd system, drilling, operator, drilling fluids and materials, strength, conformance-while-drilling technology Subjects: Drilling Operations, Pressure Management, Drilling Fluids and Materials, Well control, Drilling fluid management & disposal This content is only available via PDF. 1999. Society of Petroleum Engineers You can access this article if you purchase or spend a download.
Renal Toxicity of Interleukin-2 Administration in Patients With Metastatic Renal Cell Cancer: Effect of Pre-therapy Nephrectomy (1989)

Systemic administration of interleukin-2 and lymphokine-activated killer cells is a new approach to the immunotherapy of advanced cancer. Metastatic renal cell cancer is one of the histological types of tumors particularly susceptible to this treatment approach although renal toxicity often is a dose-limiting side effect. We compared the renal functional changes observed during interleukin-2 therapy in 52 consecutive patients with advanced renal cancer to that of 83 consecutive patients with metastatic nonrenal cancer. Of the 52 patients with renal cancer 41 had recently undergone nephrectomy. The over-all peak serum creatinine values and the percentage increase of serum creatinine over baseline for all patients studied were significantly higher in cycle 2 of interleukin-2 therapy than in cycle 1: 3.8 +/- 0.2 versus 2.6 +/- 0.1 mg. per dl. and 241.7 +/- 16.5 versus 140.3 +/- 11.0 per cent, respectively. In patients with pre-therapy serum creatinine values of 0.4 to 0.9 mg. per dl. there were no significant differences in the mean peak serum creatinine nor in the percentage increase over baseline between renal and nonrenal cancer patients during cycle 1. In cycle 2 of therapy these values were higher in the renal cancer group (3.6 +/- 0.8 versus 2.4 +/- 0.2 mg. per dl. and 310.4 +/- 103.5 versus 214 +/- 30.4 per cent, respectively) but they did not reach statistical significance (P2 = 0.08 and 0.25, respectively). Renal and nonrenal cancer patients with pre-therapy serum creatinine levels of 1.0 to 1.4 mg. per dl. achieved similar high values in cycle 2 of interleukin-2 therapy (3.9 +/- 0.3 versus 3.9 +/- 0.4 mg. per dl. and 222.7 +/- 23.2 versus 248.7 +/- 33.5 per cent, respectively), although the initial increase (cycle 1) was higher in the renal cancer patients (3.3 +/- 0.3 versus 2.4 +/- 0.2 mg. per dl. and 172.3 +/- 25.9 versus 116.1 +/- 18.0 per cent, respectively). Baseline serum creatinine greater than or equal to 1.5 mg. per dl. was associated with an over-all higher peak serum creatinine and higher percentage increase of serum creatinine over baseline than that below 1.5 mg. per dl. baseline: 4.4 mg.per dl. and 171.1 +/- 36.3 per cent in cycle 1 and 6.5 +/- 0.7 mg. per dl. and 296.1 +/- 44.0 per cent in cycle 2, respectively (p less than 0.01). There was no association between peak serum creatinine and interval from nephrectomy to interleukin-2 therapy.(ABSTRACT TRUNCATED AT 400 WORDS)