gebhart/patrepeval huggingface dataset:
Design of Trypanosoma rangeli sialidase mutants with improved trans-sialidase activity (2017)

A sialidase (EC 3.2.1.18) from the non-pathogenic Trypanosoma rangeli, TrSA, has been shown to exert trans-sialidase activity after mutation of five specific amino acids in the active site (M96V, A98P, S120Y, G249Y, Q284P) to form the so-called TrSA5mut enzyme. By computational and hypothesis driven approaches additional mutations enhancing the trans-sialidase activity have been suggested. In the present work, we made a systematic combination of these mutations leading to seven new variants of the T. rangeli sialidase, having 6-16 targeted amino acid mutations. The resulting enzyme variants were analyzed via kinetics for their ability to carry out trans-sialidase reaction using CGMP and D-lactose as substrates. The sialidase variants with 15 and 16 mutations, respectively, exhibited significantly improved trans-sialidase activity for D-lactose sialylation. Our results corroborate, that computational studies of trans-glycosylation can be a valuable input in the design of novel trans-glycosidases, but also highlight the importance of experimental validation in order to assess the performance. In conclusion, two of the seven mutants displayed a dramatic switch in specificity from hydrolysis towards trans-sialylation and constitute the most potent trans-sialidase mutants of TrSA described in literature to date.
The 6-minute walking test and all-cause mortality in patients undergoing a post-cardiac surgery rehabilitation program (2013)

In recent cardiac surgery patients, the pre-discharge 6mWT is not only a valid measurement of the impact of cardiac rehabilitation but also provides outcome information offering the possibility to identify patients who may need more intensive follow-up.
From Monomer to Conjugated Polymer: A Perspective on Best Practices for Synthesis (2021)

Conjugated polymers are a class of innovative materials used for organic photovoltaics (OPV), organic thermoelectrics (OTE), supercapacitors, electrochromics, and more. The focus of most literature reports is often given to a limited set of metrics: power conversion efficiency for OPV or conductivity and Seebeck coefficient for OTE. If a material fails to deliver a certain threshold of performance, publications concerning it may be hindered, not pursued by others in the field, or worse, not published at all. We believe more thorough preparation, purification, and purity confirmation protocols, along with careful reporting in manuscripts, would benefit the entire conjugated materials field. This contribution intends to provide practical guidance on preparation and purity to chemists synthesizing conjugated polymers. While the focus is on polymers synthesized by the Stille polymerization, many of the principles and tips apply to the synthesis of molecular species and other Pd-catalyzed cross-coupling reactions and is the fruit of two doctoral dissertations worth of education, experience, tips, and tricks. We encourage readers to use it as a starting guide and a quick reference for lab work, pin it to your lab’s bulletin board, and add your own experience to it. It is our hope that we will hold ourselves, our lab mates, our collaborators, and our field to an elevated standard of synthesis and reporting.
The Influence of Inclusions on Low Cycle Fatigue Life in a P/M Nickel-Base Disk Superalloy (1996)

The high alloy content of advanced nickel-base disk superalloys calls for powder metallurgy (P/M) processing to minimize segregation produced by conventional cast & wrought processing. Although the technology has developed to allow reliable application of P/M materials, of concern are ceramic inclusions which are intrinsic to the process due to the use of ceramic crucibles in producing the raw meltstock and in the powder atomization process itself.
Safety and feasibility of a PAclitaxel-eluting balloon angioplasty in Primary Percutaneous coronary intervention in Amsterdam (PAPPA): one-year clinical outcome of a pilot study (2014)

This first study of a DCB angioplasty-only strategy in the setting of PPCI showed good one-year clinical results.
Super directive antennas for low electromagnetic field mobile communications (2014)

This paper investigates electromagnetic field (EMF) exposure in heterogeneous cellular networks, where clusters of small cells are deployed to create local hotspots inside the macro cell. In the past, most of the research has focused on mitigating inter-cell interference and reducing the network energy consumption. However, the expected dense deployment of small cells might increase the public concern on EMF, and urge novel management techniques and hardware solutions to mitigate the EMF exposure while satisfying the requirements of quality of service. Hence, we propose to integrate small cells equipped with directive antenna to reduce the required uplink power transmission at end users to limit the associated EMF exposure. We discuss the technical challenges and highlight the design options of this approach, and we present the FP7 LEXNET methodology to evaluate the daily exposure at mobile users. Finally, a preliminary performance evaluation quantifies the benefits of our solution.
Effects of resonant mode coupling on optical Characteristics of InGaN-GaN-AlGaN lasers (2005)

The phenomenon of resonant mode coupling is modeled and numerically investigated in InGaN-based multiple-quantum-well laser structures. It is shown that under resonant conditions, the internal mode coupling can lead to severe distortions of both near- and far-field characteristics. Taking into account the carrier-induced change of the modal effective index for the lasing mode, we show that the resonant internal mode coupling can result in a strongly nonlinear dependence of the modal gain on injection current, with possible region of negative differential modal gain.
Preliminary analysis of the use of smartwatches for longitudinal health monitoring (2015)

New generations of smartwatches feature continuous measurement of physiological parameters, such as heart rate, galvanic skin resistance (GSR), and temperature. In this paper we present the results of preliminary analysis of the use of Basis Peak smartwatch for longitudinal health monitoring during a 4 month period. Physiological measurements during sleep are validated using Zephyr Bioharness 3 monitor and SOMNOscreen+ polysomnographic monitoring system from SOMNOmedics. Average duration of sequences with no missed data was 49.9 minutes, with maximum length of 17 hours, and they represent 88.88% of recording time. Average duration of the charging event was 221.9 min, and average time between charges was 54 hours, with maximum duration of the charging event of 16.3 hours. Preliminary results indicate that the physiological monitoring performance of existing smartwatches provides sufficient performance for longitudinal monitoring of health status and analysis of health and wellness trends.
NMR studies on Fraser fir Abies fraseri (Pursh) Poir. Lignins (2005)

Abstracting & Indexing
A Technical and Economical Evaluation of CO2Capture from Fluidized Catalytic Cracking (FCC) Flue Gas (2014)

Environmental issues, related to greenhouse gas and among them CO<sub>2<sub/>, are becoming short term challenges. Pressure on industries and therefore on refining to limit and manage CO<sub>2<sub/> emissions will be reinforced in next few years.Refining industry is responsible for about 2.7% of global CO<sub>2<sub/> emissions. Fluidized Catalytic Cracking unit (FCC), one of the main process in refining, represents by itself 20% of the refinery CO<sub>2<sub/> emissions. As FCC unit is present in half of the refining schemes, it is challenging to find technologies to manage its emissions.Based on an industrial case, the aims of the presented work are to determine if amine technology HiCapt<sup>+<sup/>, developed for power plant, might be a relevant solution to manage FCC CO<sub>2<sub/> emissions and to evaluate the additional cost to be supported by refiners.
High-k Fluoropolymer Gate Dielectric in Electrically Stable Organic Field-Effect Transistors (2019)

A detailed study of a high-k fluoropolymer gate dielectric material, poly(vinylidene fluoride- co-hexafluoropropylene) [P(VDF-HFP)], is presented as a guide to achieve low operational voltage and electrically stable device performance. The large dipole moment of C-F dipoles in P(VDF-HFP) is responsible for its high dielectric constant as well as its potentially ferroelectric behavior that must be minimized to avoid hysteretic current-voltage characteristics. A range of material grades and processing conditions are explored and are shown to have a significant effect on the degree of hysteresis observed in device-transfer characteristics. The percentage of HFP monomer in the P(VDF-HFP) dielectric has an effect on gate-dependent mobility induced by disorder at the semiconductor-dielectric interface. Most importantly, we present the considerations that must be made to achieve optimal performance in multiple device architectures of organic field-effect transistors when using P(VDF-HFP) as a dielectric layer.
Changes in conformation and slow refolding kinetics in mutant iso-2-cytochrome c with replacement of a conserved proline residue (1987)

Using oligonucleotide-directed mutagenesis, we have produced a mutant form of iso-2-cytochrome c of yeast in which threonine (Thr-71) replaces a conserved proline residue (Pro-71) located between two short alpha-helical segments in the native protein. Optical spectroscopy indicates that, at pH 7.2, Thr-71 iso-2-cytochrome c folds to a nonnative conformation possibly related to the alkaline form of the native protein. On titration to pH 5.2, Thr-71 iso-2-cytochrome c regains many of the optical properties of the normal protein. We have shown that the proline residue at position 71 has no effect on the kinetics of fluorescence-detected slow refolding. However, between pH 5 and pH 7.2 the amplitude for absorbance-detected slow folding is strongly pH dependent in the mutant protein but is largely independent of pH in the normal protein. We believe this to be due to the folding of Thr-71 iso-2-cytochrome c to a nonnative conformation at pH 7.2 that does not require the slow, absorbance-detected conformational changes observed in folding to the more native-like state at pH 5-6.
Material mechanical characterization method for multiple strains and strain rates (2023)

A specimen for measuring a material under multiple strains and strain rates. The specimen including a body having first and second ends and a gage region disposed between the first and second ends, wherein the body has a central, longitudinal axis passing through the first and second ends. The gage region includes a first gage section and a second gage section, wherein the first gage section defines a first cross-sectional area that is defined by a first plane that extends through the first gage section and is perpendicular to the central, longitudinal axis. The second gage section defines a second cross-sectional area that is defined by a second plane that extends through the second gage section and is perpendicular to the central, longitudinal axis and wherein the first cross-sectional area is different in size than the second cross-sectional area.
Engineered modular heterocyclic-diamidines for sequence-specific recognition of mixed AT/GC base pairs at the DNA minor groove (2021)

This report describes a breakthrough in a project to design minor groove binders to recognize any sequence of DNA. A key goal is to invent synthetic chemistry for compound preparation to recognize an adjacent GG sequence that has been difficult to target. After trying several unsuccessful compound designs, an <i>N</i>-alkyl-benzodiimidazole structure was selected to provide two H-bond acceptors for the adjacent GG-NH groups. Flanking thiophenes provide a preorganized structure with strong affinity, DB2831, and the structure is terminated by phenyl-amidines. The binding experimental results for DB2831 with a target AAAGGTTT sequence were successful and include a high Δ<i>T</i> <sub>m</sub>, biosensor SPR with a <i>K</i> <sub>D</sub> of 4 nM, a similar <i>K</i> <sub>D</sub> from fluorescence titrations and supporting competition mass spectrometry. MD analysis of DB2831 bound to an AAAGGTTT site reveals that the two unprotonated <i>N</i> of the benzodiimidazole group form strong H-bonds (based on distance) with the two central G-NH while the central -CH of the benzodiimidazole is close to the -C[double bond, length as m-dash]O of a C base. These three interactions account for the strong preference of DB2831 for a -GG- sequence. Surprisingly, a complex with one dynamic, interfacial water is favored with 75% occupancy.
Big-data-driven network partitioning for ultra-dense radio access networks (2017)

The increased density of base stations (BSs) may significantly add complexity to network management mechanisms and hamper them from efficiently managing the network. In this paper, we propose a big-data-driven network partitioning and optimization framework to reduce the complexity of the networking mechanisms. The proposed framework divides the entire radio access network (RAN) into multiple sub-RANs and each sub-RAN can be managed independently. Therefore, the complexity of the network management can be reduced. Quantifying the relationships among BSs is challenging in the network partitioning. We propose to extract three networking features from mobile traffic data to discover the relationships. Based on these features, we engineer the network partitioning solution in three steps. First, we design a hierarchical clustering analysis (HCA) algorithm to divide the entire RAN into sub-RANs. Second, we implement a traffic load balancing algorithm to characterize the performance of the network partitioning. Third, we adapt the weights of networking features in the HCA algorithm to optimize the network partitioning. We validate the proposed solution through simulations designed based on real mobile network traffic data. The simulation results reveal the impacts of the RAN partitioning on the networking performance and the computational complexity of the networking mechanism.
Molybdenum containing scintillating bolometers for double-beta decay search (LUMINEU program) (2015)

A scintillating bolometer technology, promising to be used in a next-generation cryogenic experiment to search for neutrinoless double-beta decay, is currently under development within the LUMINEU (Luminescent Underground Molybdenum Investigation for NEU-trino mass and nature) program. The recent results about the R&amp;D; of high quality large volume molybdenum containing crystal scintillators (zinc and lithium molybdates), including ones produced from 100 Mo-enriched powder, and aboveground / underground tests of cryogenic detectors based on these crystals are presented here.
Noise Investigation of Deep-UV Dispersive Wave Generation in Gas-Filled Fiber Utilizing Nonlinear Pulse Compression (2020)

Deep-UV radiation is achieved through dispersive wave generation in an argon-filled anti-resonant fiber utilizing a nonlinearly compressed 1030nm pump. Relative intensity noise is characterized at 280nm, with 1.6% achieved for optimal argon-pressure and pump parameters.
High-Q photonic band gap resonant cavities: from mm-wave to optical regime (1996)

We have realized a new class of high-Q resonant cavity using two-dimensional photonic bandgap (PBG) structures and showed that its Q-value can be as high as approximately 23,000 in the mm-wave regime. We further show that its modal properties, such as the resonant frequency, modal linewidth and number of modes, can be tuned by varying the cavity size. In addition, we present a new nano-fabrication technique for constructing PBG resonant cavities in the near infrared and visible spectral regime.
EXAMINATION OF A CASE OF SUSPECTED McARDLEʼS SYNDROME BY 31P NUCLEAR MAGNETIC RESONANCE. (1982)

Ross, B. D.; Radda, G. K.; Gadian, D. G.; Rocker, G.; Esiri, M.; Falconer-Smith, J.Author Information
Thermal management of microelectronic equipment : heat transfer theory, analysis methods, and design practices (2002)

With an increased demand on system reliability and performance combined with the miniaturization of devices, thermal consideration has become a crucial factor in the design of electronic packaging, from chip to system levels. This book emphasizes the solving of practical design problems in a wide range of subjects related to various heat transfer technologies
Effectiveness and Safety of a Novel Care Model for the Management of Type 2 Diabetes at 1 Year: An Open-Label, Non-Randomized, Controlled Study (2018)

Virta Health Corp.
Understanding magnetic interactions and reversal mechanisms in a spinodally decomposed cobalt ferrite using first order reversal curves (2023)

Cobalt ferrites exhibit widely varied magnetic behaviour due to the presence of a miscibility gap leading to the formation of periodic self-assembled nanostructures via spinodal decomposition. Periodicity and amplitude of the compositional fluctuations can be controlled by thermodynamic and kinetic processing parameters which allows for careful tuning of the magnetic properties. Although reports have shown evidence of spinodal decomposition, there is a lack of detailed characterization of the magnetic interactions and reversal mechanisms in these materials. In this work we use high-resolution first order reversal curves (FORC) measurements to understand the underlying magnetic processes occurring in a cobalt ferrite with a nominal composition of Co1.8Fe1.2O4 before (calcined) and after spinodal decomposition (annealed). Additionally, FORC measurements with preconditioning fields were conducted to separate the interaction signatures at low coercive fields by biasing the sample in positive and negative mean fields. Microstructural characterization using TEM combined with EDS showed uniform chemistry in the calcined sample and the presence of Fe rich and Co rich regions in the annealed sample, due to spinodal decomposition. Signs of positive exchange interactions were observed in both calcined and annealed samples. This work presents the first detailed magnetic characterization of magnetic interactions in a nanostructured cobalt ferrite, and provides an example of magnetic characterization of nanostructured ferrites using FORC.
Trends and Clinical Practice Patterns of Sacral Neuromodulation for Overactive Bladder (2017)

Sacral neuromodulation has increased dramatically over the past decade in surgeons certified with the ABU. This is in contrast to AC, which while remaining stable in number of procedures.
Abschätzung der Strahlenexposition der 128-Zeilen 4D Perfusions-CT zur Evaluation der Tumorvaskularität (2010)

Ziele: Ziel der Studie war die Abschätzung der effektiven Ganzkörperdosis und der organspezifischen Dosen der 128-Zeilen 4D Perfusions-CT der Lunge, der Leber und des Beckens. Methode: Ein mit Thermolumineszenzdosimetern bestücktes Alderson-Rando-Phantom wurde für die Messungen verwendet. Entsprechend der Richlinien von ICRP 103 wurde die effektive Dosis berechnet. Alle Messungen wurden an einem 128-Zeilen Scanner (Siemens Definition AS+, Forchheim, Deutschland) im adaptiven 4D Spiralmodus mit bidirektionaler Tischbewegung und einer Scanstrecke von 69mm über eine Zeitperiode von etwa 120 s (26 Scans) durchgeführt. Perfusionsmessungen für die Lunge, die Leber und das Becken wurden mit folgenden Parametern simuliert: Lunge (80kV, 60mAs), Leber (80kV, 80mAs), Becken (100kV, 80mAs). Ergebnis: Abhängig vom Geschlecht und der untersuchten Körperregion variiert die effektive Ganzkörperdosis zwischen 2,9 und 7,1 mSv. Für geschlechtsspezifische Organe im primären Strahlenfeld wie die weibliche Brust (Lungenperfusion) oder Ovarien (Beckenperfusion) wurden effektive Organdosen von bis zu 3,0 mSv gemessen. Dies verursacht einen Anstieg der frauenspezifischen effektiven Dosis um bis zu 85,7% beziehungsweise 100,0% im Vergleich zur effektiven Ganzkörperdosis von Männern. Schlussfolgerung: Aufgrund applizierter effektiver Ganzkörperdosen von bis zu 7,1 mSv ist der verantwortungsvolle Umgang mit der vielversprechenden neuen Technik der 4D Perfusions-CT notwendig. Geschlechts- und organspezifische Unterschiede sollten gerade bei jüngeren Patienten bei der Planung von Tumorperfusionen berücksichtigt werden.
Molecular Mapping of Soybean Nodulation Genes (2020)

Soybean is one of the major food crops. It supplies plant protein for human and animal consumption, but also vegetable oils. The study of plant genes involved in the symbiosis advanced rapidly with the introduction of molecular biological techniques. The most significant finding from the physical mapping study was the calculation that one centiMorgan in this region of the soybean genome was equivalent to about 500 kb of DNA. Nodulation and nitrogen fixation contribute substantially to the nitrogen balance of soybean crops. The plant analysis of nodulation has been restricted by the absence of sufficient genetic variation in the nodulation phenotype, and inaccurate methods for the quantitative measurement of nitrogen fixation in the whole plant. Nodulation analysis provides information which has relevance to the whole field of biological research. Fundamental processes like cell division control, tissue-specific gene expression and plant immune response are addressed by this type of research.
The gut microbial metabolic capacity of microbiome-humanized vs. wild type rodents reveals a likely dual role of intestinal bacteria in hepato-intestinal schistosomiasis (2022)

Increasing evidence shows that the host gut microbiota might be involved in the immunological cascade that culminates with the formation of tissue granulomas underlying the pathophysiology of hepato-intestinal schistosomiasis. In this study, we investigated the impact of Schistosoma mansoni infection on the gut microbial composition and functional potential of both wild type and microbiome-humanized mice. In spite of substantial differences in microbiome composition at baseline, selected pathways were consistently affected by parasite infection. The gut microbiomes of infected mice of both lines displayed, amongst other features, enhanced capacity for tryptophan and butyrate production, which might be linked to the activation of mechanisms aimed to prevent excessive injuries caused by migrating parasite eggs. Complementing data from previous studies, our findings suggest that the host gut microbiome might play a dual role in the pathophysiology of schistosomiasis, where intestinal bacteria may contribute to egg-associated pathology while, in turn, protect the host from uncontrolled tissue damage.
Supplementary Table 1 from Comparison of Human and Rat Uterine Leiomyomata: Identification of a Dysregulated Mammalian Target of Rapamycin Pathway (2023)

Supplementary Table 1 from Comparison of Human and Rat Uterine Leiomyomata: Identification of a Dysregulated Mammalian Target of Rapamycin Pathway
Hash image transform for accelerated screen content encoding in HEVC (2016)

In this paper, we present a hash-based framework for acceleration of a block-based hybrid video encoder. The framework consists of a hash-based transform, a hash-based motion analysis, and a finial collaboration of the analysis information for different block sizes. The hash-based transform generates an image where each sample represents a complete block. Thus, in the hash-based motion analysis, only a comparison of samples from the hash images is performed instead of complete blocks of the actual images. The results from the motion analysis are collaborated for different block sizes, which is then used to guide a block-based video encoder. The proposed framework was implemented on top of HM-16.7SCM-6.0 and has been evaluated for encoding of screen content video signals. Simulation results show that the proposed framework achieves significant runtime reductions of up to 82.8% while approximately keeping or lowering the bitrate by up to 6.1% compared to the HEVC reference software HM-16.7_SCM-6.0 for low-delay-P-frame coding.