gebhart/patrepeval huggingface dataset:
Molecular Genetics in Acromegaly (2016)

Abstract Pituitary tumours are the most frequent intracranial neoplasms, affecting 1/1000 of the worldwide population. Growth hormone (GH)‐secreting adenomas, also known as somatotropinomas, comprise about 20% of all pituitary tumours. Acromegaly is the clinical syndrome that results from high levels of GH in patients after the end of their growing years, whereas gigantism occurs when high GH acts on a skeleton that is still growing. Most cases of acromegaly/gigantism occur as a result of sporadic GH‐secreting pituitary adenomas. However, in 5% of cases (and significantly more frequently in the young ages), they can occur as a result of a genetic defect, most of the time in association with other endocrine abnormalities or as an isolated disorder. These familial syndromes include multiple endocrine neoplasia (MEN) type 1 (MEN1), Carney complex (CNC), MEN type 4 (MEN4). McCune–Albright syndrome (MAS), familial isolated pituitary adenoma (FIPA), the 3Ps association (pheochromocytoma/paraganglioma and pituitary adenoma syndrome or 3PAs) and X‐linked acrogigantism (X‐LAG). Key Concepts About 5% of acromegaly may be caused by a genetic mutation; this may be significantly higher in the young ages. The frequency of GH‐producing pituitary tumours in MEN1 syndrome is only about 10%, with a mean age at diagnosis comparable to the sporadic GH‐secreting adenomas (40–45 years) but with a more aggressive behaviour. The incidence of GH‐producing pituitary tumours in Carney complex (CNC) has been estimated to be less than 15%; however, insulin‐like growth factor 1 (IGF‐1) elevation may be present in up to 80% of affected patients who have no detectable tumours due to somatomammotrophic hyperplasia, which may precede clinically evident acromegaly. GH excess is found in up to 20% of patients with McCune–Albright syndrome mainly due to GH‐producing cell hyperplasia rather than due to pituitary tumours, which are identified in only a few patients. Somatotropinomas are the most common tumour type in both AIP mutation‐positive and ‐negative familial isolated pituitary adenoma (FIPA) families. AIP mutation‐positive FIPA patients are more likely to have larger tumours, more likely to invade the extrasellar region and more resistant to medication treatment GH‐secreting pituitary adenomas in 3PAs due to SDHx mutations display more aggressive phenotype, are more resistant to somatostatin analogues, and most often require surgery X‐linked acrogigantism (X‐LAG) caused by an Xq26.3 genomic duplication is a paediatric disorder characterised by early onset gigantism resulting from an excess of growth hormone as early as in infancy. The GPR101 gene duplication most likely causes X‐LAG.
Gas phase hydrogen/deuterium exchange reactions of peptide ions in a quadrupole ion trap mass spectrometer (1997)

Hydrogen/deuterium exchange reactions of protonated and sodium cationized peptide molecules have been studied in the gas phase with a MALDI/quadrupole ion trap mass spectrometer. Unit-mass selected precursor ions were allowed to react with deuterated ammonia introduced into the trap cell by a pulsed valve. The reactant gas pressure, reaction time, and degree of the internal excitation of reactant ions were varied to explore the kinetics of the gas phase isotope exchange. Protonated peptide molecules exhibited a high degree of reactivity, some showing complete exchange of all labile hydrogen atoms. On the contrary, peptide molecules cationized with sodium exhibited only very limited reactivity, indicating a vast difference between the gas phase structures of the two ions.
Sound source localization for subband-based two speech separation in room environment (2013)

This paper investigates the problem of subband speech separation from a mixture of two speech signals in a room environment. Due to the lack of source information, a sound source localization is proposed for beamformer design in each subband to obtain a good interference suppression with negligible expense on each target signal integrity. Evaluations on real speech data show that the proposed speech separation method offers a good interference suppression level whilst maintaining a low distortion level of the target source.
On right-hand uniqueness of solutions to nondegenerated algebro-differential equations with discontinuances (2007)

A finite-dimensional nondegenerate system of algebro-differential equations with discontinuances is considered. An equivalent system of discontinuous differential equations is constructed. Conditions of right-hand uniqueness of solution and of convergence of numerical methods are given.
Eicosanoids and nitric oxide influence induction of reactive gliosis from spreading depression in microglia but not astrocytes (1996)

Microglia and astrocytes are transformed into reactive glia (RG) by brain disease and normal function. Eicosanoids and nitric oxide (NO), two intercellular mediators, may influence gliosis. We investigated how drugs that alter production of these paracrine signals effect induction of glial reactivity from spreading depression. Unilateral (left) neocortical spreading depression was induced in 95 halothane anesthetized rats by intracortical injections of 0.5 M KCl, with or without drug treatment (five animals/group). Immunohistochemical staining (IS) intensity using the OX-42 and anti-glial fibrillary acidic protein (GFAP) antibodies determined reactivity in microglia and astrocytes, respectively. After 3 days, brains were processed for OX-42 and GFAP-IS and mean optical densities (OD) of IS were measured. Average OD's (for OX-42) and the log ratio (left/right) of OD's (OX-42 and GFAP) were compared to normal animals. Spreading depression induced significant log ratios for both OX-42- and GFAP-IS (P's < 0.01). However, dexamethasone (a glucocorticoid), nordihydroguaiaretic acid (a lipoxygenase inhibitor), and nitroprusside (a NO donor) prevented significant left sided and log ratio OD values for microglia (P's > 0.05). L-Name, a NO synthase inhibitor, caused significant increases in left and right OD's for microglia (P's < 0.05). Mepacrine, a phospholipase A2 inhibitor, Indomethacin, a cyclooxygenase inhibitor, and phenylephrine, an adrenergic agonist, did not prevent induction of significant OX-42 log ratios (P's < 0.01, 0.05, 0.01), and resulted in increases in left side OD's (P's < 0.01, 0.05, 0.05). Significant GFAP log ratios occurred after spreading depression in all drug groups, P's < 0.01. Thus, induction of reactivity in microglia is more sensitive to eicosanoids and NO than in astrocytes.
Primary structure and functional expression of mouse pituitary and human brain corticotrophin releasing factor receptors (1993)

Corticotrophin-releasing factor (CRF) is the principal hypothalamic factor governing the pituitary-adrenal axis, but the wide extra-pituitary distribution of CRF and its receptors suggest a major role for this neuropeptide in the integration of the overall physiological and behavioral responses of an organism to stress. We have cloned a CRF receptor complementary DNA (cDNA) by expression in COS-7 cells of a cDNA library from the AtT20 mouse pituitary tumour cell line. The cloned mouse cDNA was then as a probe to isolate a human CRF receptor cDNA from a human brain cDNA library. The mouse and human cDNAs both encode 415 amino acid proteins that are 97% identical, containing seven putative transmembrane domains characteristic of G protein-coupled receptors. The CRF receptor shows homology with the receptors for growth hormone-releasing factor, vasoactive intestinal peptide, secretin, parathyroid hormone, and calcitonin. COS-7 cells transfected with the mouse CRF receptor cDNA bind radiolabelled ovine CRF with high affinity and respond specifically to CRF by accumulation of intracellular cAMP. A 2.7 kb mRNA coding for the CRF receptor could be detected in AtT20 cells and human cortex tissue. PCR analysis also detected the receptor transcript in human pituitary, brainstem, and testis.
Learning Monocular Visual Odometry via Self-Supervised Long-Term Modeling (2020)

Monocular visual odometry (VO) suffers severely from error accumulation during frame-to-frame pose estimation. In this paper, we present a self-supervised learning method for VO with special consideration for consistency over longer sequences. To this end, we model the long-term dependency in pose prediction using a pose network that features a two-layer convolutional LSTM module. We train the networks with purely self-supervised losses, including a cycle consistency loss that mimics the loop closure module in geometric VO. Inspired by prior geometric systems, we allow the networks to see beyond a small temporal window during training, through a novel a loss that incorporates temporally distant (e.g., O(100)) frames. Given GPU memory constraints, we propose a stage-wise training mechanism, where the first stage operates in a local time window and the second stage refines the poses with a "global" loss given the first stage features. We demonstrate competitive results on several standard VO datasets, including KITTI and TUM RGB-D.
Photosynthetic Complexes: Molecularly Activated Bioswitches and Agents for Light Powered Molecular Circuitry (2004)

Abstract : This program was directed at demonstrating functional optoelectronic devices by contacting individual, highly photoactive molecules of biological origin. This 2 phase program (each phase duration of 1 year), successfully addressed the fundamental engineering issues associated with the application of protein/molecular aggregates to electronic devices: purification of the complexes, and their interconnection to conventional bulk organic circuitry. We have demonstrated a molecular power source using a single antenna complex extracted from a biological photosynthetic unit. To exploit the optimization of photosynthesis, we have demonstrated, for the first time, the solid state integration of photosynthetic complexes in solar cells. The internal quantum efficiency of the first generation of devices is estimated to be 12%.
Thermal Management of a Soft Starter: Transient Thermal Impedance Model and Performance Enhancements Using Phase Change Materials (2010)

Adverse effects of starting-torque transients and high in-rush currents in induction motors are typically mitigated by employing electronically controlled soft starting voltages through silicon-controlled rectifiers (SCRs). However, the heat dissipation in the soft starter must be carefully managed in the design of motor drives. The objective of this study is both to address the heat dissipation in the soft starter by implementing analytical solutions to the heat diffusion equations inside the soft starter, and to investigate the use of a phase change material (PCM) based heat sink for thermal management of the device. The analytical modeling approach is, however, general, and can be applied to the solution of a range of thermal problems in power electronics. The transient analytical thermal model, based on the thermal quadrupole approach, allows a determination of the transient performance of a soft starter by evaluating the thyristor junction temperature. Predictions from the model are first compared to results obtained using a coupled thermal and electrical model based on a resistance/capacitance network approach. Experimental results obtained with the soft starter connected to a low-voltage 200 hp induction machine are then used to validate the model. Additionally, the performance improvement resulting from the use of a hybrid heat sink (plate fin heat sink immersed in a PCM) is evaluated and compared to a conventional air-cooled heat sink without a PCM under identical conditions.
Hodgkin’s Disease in Costa Rica:A Report of 40 Cases Analyzed for Epstein-Barr Virus (1998)

We studied 40 cases of Hodgkin's disease (HD) from Costa Rica for evidence of Epstein-Barr virus (EBV) in the Reed-Sternberg and Hodgkin (RS-H) cells. We also compared the epidemiologic features of these patients with previous reports of HD in industrialized and developing nations. Because Costa Ricans enjoy a relatively higher standard of living than the residents of other developing Central American nations yet live in the same general geographic region and are genetically similar, we believed that this comparison might shed additional light on the hypothesis that the prevalence of EBV in HD and the epidemiologic factors of HD are influenced by socioeconomic factors. In 16 (40%) of 40 cases, immunohistochemical studies demonstrated that the RS-H cells were positive for EBV latent membrane protein (LMP), including 1 case of lymphocytic depletion analyzed, 12 (86%) of 14 cases of mixed cellularity, and 3 (15%) of 20 cases of nodular sclerosis. All five cases of lymphocytic predominance were negative. In the 16 EBV LMP-positive cases, polymerase chain reaction studies revealed that the virus was type A in 12 cases and type B in 4 cases. Nodular sclerosis was the most common type of HD, accounting for 20 cases (50%), followed by mixed cellularity, with 14 cases (35%). The relatively low prevalence of EBV in the RS-H cells of HD and the high incidence of nodular sclerosis in Costa Rica are similar to industrialized nations and are unlike HD in neighboring Central American countries. These findings further support the hypothesis that the prevalence of EBV in HD and the epidemiologic features of HD are most closely linked with socioeconomic conditions, and geographic location or ethnic heritage are of relatively less importance.
Direct Observations of Sigma Phase Growth and Dissolution in 2205 Duplex Stainless Steel (2005)

The formation and growth of sigma ({sigma}) phase in a 2205 duplex stainless steel is monitored during an 850 C isothermal heat treatment using an in situ synchrotron x-ray diffraction technique. At this temperature, {sigma} phase is first observed within approximately 40 seconds of the start of the isothermal heat treatment and grows rapidly over the course of the 3600 second heat treatment to a volume fraction of approximately 13%. A simultaneous increase in the austenite ({gamma}) volume fraction and a decrease in the ferrite ({delta}) volume fraction are observed. The {sigma} phase formed at this temperature is rapidly dissolved within approximately 200 seconds when the temperature is increased to 1000 C. Accompanying this rapid dissolution of the {sigma} phase, the {delta} and {gamma} volume fractions both approach the balanced (50/50) level observed in the as-received material.
Strategies for increasing lipid accumulation and recovery fromY. lipolytica: A review (2021)

Microbial-based biodiesel is produced by transesterification of lipids extracted from microbial cells, and is considered as a potential replacement of fossil fuel due to its advantages in reducing greenhouse gas emissions. Yarrowia lipolytica is one of the most studied oleaginous yeasts able to produce lipids under some fermentation conditions and is considered as a potential industrial host for biodiesel production. Several approaches have been evaluated to increase the economical attraction of biodiesel production from Y. lipolytica lipids. In this review, we highlighted the different strategies reported in the literature, allowing this yeast to achieve high lipid accumulation. These include metabolic engineering strategies, the use of low-cost effective substrates, and the optimization of the cultivation conditions for higher lipid productivity and less operating cost. We also summarized the most effective cell disruption technologies that improve the extraction efficiencies of lipids from Y. lipolytica .
Robust thalamic nuclei segmentation method based on local diffusion magnetic resonance properties (2016)

The thalamus is an essential relay station in the cortical-subcortical connections. It is characterized by a complex anatomical architecture composed of numerous small nuclei, which mediate the involvement of the thalamus in a wide range of neurological functions. We present a novel framework for segmenting the thalamic nuclei, which explores the orientation distribution functions (ODFs) from diffusion magnetic resonance images at 3 T. The differentiation of the complex intra-thalamic microstructure is improved by using the spherical harmonic (SH) representation of the ODFs, which provides full angular characterization of the diffusion process in each voxel. The clustering was performed using the k-means algorithm initialized in a data-driven manner. The method was tested on 35 healthy volunteers and our results show a robust, reproducible and accurate segmentation of the thalamus in seven nuclei groups. Six of them closely matched the anatomy and were labeled as anterior, ventral anterior, medio-dorsal, ventral latero-ventral, ventral latero-dorsal and pulvinar, while the seventh cluster included the centro-lateral and the latero-posterior nuclei. Results were evaluated both qualitatively, by comparing the segmented nuclei to the histological atlas of Morel, and quantitatively, by measuring the clusters' extent and the clusters' spatial distribution across subjects and hemispheres. We also showed the robustness of our approach across different sequences and scanners, as well as intra-subject reproducibility of the segmented clusters using additional two scan-rescan datasets. We also observed an overlap between the path of the main long-connection tracts passing through the thalamus and the spatial distribution of the nuclei identified with our clustering algorithm. Our approach, based on SH representations of the ODFs, outperforms the one based on angular differences between the principle diffusion directions, which is considered so far as state-of-the-art method. Our findings show an anatomically reliable segmentation of the main groups of thalamic nuclei that could be of potential use in many clinical applications.
Role of the atrial natriuretic peptide for adrenal regulation in the teleost fish Cyprinus carpio (1994)

The biological role of atrial natriuretic peptide (ANP) for the adrenal tissue of the teleost fish Cyprinus carpio, the carp, was investigated using in vitro autoradiography to study ANP binding sites, immunohistochemistry to localize ANP-like peptides, and in vitro perifusion to measure adrenal cortisol and catecholamine release. 125I-labeled rat ANP-(99-126) [125I-rANP-(99-126)] binding was present in adrenal tissue exhibiting positive cooperativity with a half-maximal binding concentration (EC50) of 87 +/- 15 pM and a maximal binding capacity (Bmax) of 56 +/- 11 amol/mm2 (n = 3). 125I-rANP-(99-126) binding was competitively displaced by unlabeled ANP analogues with an intact disulfide bridge showing a lower affinity than the iodinated ligand. By the use of immunohistochemistry, ANP immunoreactivity was found in the majority of the phenylethanolamine N-methyl-transferase-immunoreactive, i.e., epinephrine-synthesizing cells. In vitro, ANP elevated both basal and acetylcholine-stimulated cortisol release by carp adrenal tissue, whereas adrenal catecholamines were significantly affected only in acetylcholine-stimulated epinephrine secretion. Thus ANP derived from the ANP-immunoreactive chromaffin cells of the carp adrenal seems to act in an autocrine and/or paracrine manner on steroidogenic interrenal and catecholaminergic chromaffin cells. Furthermore, the study indicates that the ANP effects on fish catecholamine and steroid release are reversed in mammals.
Beam redirection and frequency filtering with transparent elastomeric diffractive elements (1999)

A new, to our knowledge, type of optical device capable of beam redirection and frequency filtering is described. It is based on a transparent elastomeric binary diffraction grating. When light is passed through the device the intensities of the diffraction orders can be modulated by compression of the elastomer in the direction perpendicular to the plane of the grating. Selective filtering of the component frequencies of two-component light (lambda = 543.5 nm and lambda = 632.8 nm) has been demonstrated. Experimental observations are in agreement with theoretical calculations quantifying the performance of the device.
Cell-Specific Expression of the Promoters of Two Nonlegume Hemoglobin Genes in a Transgenic Legume, Lotus corniculatus (1997)

The promoters of the hemoglobin genes from the nitrogen-fixing tree Parasponia andersonii and the related nonnitrogen-fixing Trema tomentosa both confer beta-glucuronidase reporter gene expression to the central zone of the nodules of a transgenic legume, Lotus corniculatus. beta-Glucuronidase expression was high in the uninfected interstitial cells and parenchyma of the surrounding boundary layer and was low in the Rhizobium-infected cells. This contrasts with the expression of both the P. andersonii hemoglobin protein in P. andersonii nodules and the endogenous Lotus leghemoglobins that are expressed in the infected cells at very high levels. The expression pattern of the P. andersonii and T. tomentosa hemoglobin promoters in L. corniculatus resembles that of a nonsymbiotic hemoglobin gene from Casuarina glauca, which was introduced into this legume, and suggests that only the nonsymbiotic functions of the P. andersonii promoter are being recognized. Deletion of the distal segments of both the P. andersonii and T. tomentosa promoters identified regions important for the control of their tissue-specific and temporal activity in Lotus. Potential regulatory elements, which enhance nodule expression and suppress nonnodule expression, were also identified and localized to a distal promoter segment. A proximal AAGAG motif is present in the P. andersonii, T. tomentosa, and nonsymbiotic Casuarina hemoglobin genes. Mutation of this motif in the P. andersonii promoter resulted in a significant reduction in both the nodule and root expression levels in L. corniculatus. Some of the regulatory motifs characterized are similar to, but different from, the nodulin motifs of the leghemoglobins.
Export of l -Isoleucine from Corynebacterium glutamicum : a Two-Gene-Encoded Member of a New Translocator Family (2002)

Bacteria possess amino acid export systems, and Corynebacterium glutamicum excretes L-isoleucine in a process dependent on the proton motive force. In order to identify the system responsible for L-isoleucine export, we have used transposon mutagenesis to isolate mutants of C. glutamicum sensitive to the peptide isoleucyl-isoleucine. In one such mutant, strong peptide sensitivity resulted from insertion into a gene designated brnF encoding a hydrophobic protein predicted to possess seven transmembrane spanning helices. brnE is located downstream of brnF and encodes a second hydrophobic protein with four putative membrane-spanning helices. A mutant deleted of both genes no longer exports L-isoleucine, whereas an overexpressing strain exports this amino acid at an increased rate. BrnF and BrnE together are also required for the export of L-leucine and L-valine. BrnFE is thus a two-component export permease specific for aliphatic hydrophobic amino acids. Upstream of brnFE and transcribed divergently is an Lrp-like regulatory gene required for active export. Searches for homologues of BrnFE show that this type of exporter is widespread in prokaryotes but lacking in eukaryotes and that both gene products which together comprise the members of a novel family, the LIV-E family, generally map together within a single operon. Comparisons of the BrnF and BrnE phylogenetic trees show that gene duplication events in the early bacterial lineage gave rise to multiple paralogues that have been retained in alpha-proteobacteria but not in other prokaryotes analyzed.