gebhart/patrepeval huggingface dataset:
Abstract 2846: Safety and efficacy of a genetic vaccine targeting telomerase plus chemotherapy for the therapy of canine B-cell lymphoma . (2013)

Abstract Background: We have recently shown that a genetic vaccine targeting dog telomerase (dTERT) and based on Ad/DNA-EGT technology can induce strong cell-mediated immune responses against this tumor antigen and increase overall survival of dogs affected by B-cell lymphosarcoma (LSA) in comparison with historical controls when combined with COP chemotherapy regimen. Objectives: Here, we have conducted a double arm clinical trial with an extended number of LSA patients, measured the antigen-specific immune response and evaluated potential toxic effects of the immunotherapy along with a follow up of patients survival for three years and half. Methods: The immune response was measured by ELISPOT. The expression of dTERT was quantified by quantitative PCR. Changes in hematological parameters, local/systemic toxicity or organic dysfunction and fever were monitored over time during the treatment. Results: dTERT-specific cell mediated immune responses were induced in almost all treated animals No adverse effects were observed in any dog patient that underwent treatment. The overall survival time of vaccine/COP treated dogs was significantly increased over the COP-only treated cohort (&amp;gt;76.1 vs 29.3 weeks, respectively, p&amp;lt;0.0001). There was a significant association between dTERT expression levels and overall survival (OS) among vaccinated patients. Conclusions and Clinical relevance: Ad/DNA-EGT-based cancer vaccine against dTERT in combination with COP chemotherapy is safe and significantly prolongs the survival of LSA canine patients. These data confirm the therapeutic efficacy of dTERT vaccine and support the evaluation of this approach in human trials and for other cancer types. Citation Format: Alessandra Gavazza, George Lubas, Arthur Fridman, Daniela Peruzzi, Joseph A. Impellizeri, Laura Luberto, Emanuele Marra, Giuseppe Roscilli, Gennaro Ciliberto, Luigi Aurisicchio. Safety and efficacy of a genetic vaccine targeting telomerase plus chemotherapy for the therapy of canine B-cell lymphoma . [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 2846. doi:10.1158/1538-7445.AM2013-2846
Statin Enhances Cisplatin Induced Effect on Hepatoma Cell Lines (2008)

Purpose: Hepatocellular carcinoma (HCC) is the fifth most common malignancy worldwide and continues to pose a therapeutic challenge. For those unable to undergo transplantation, radiofrequency ablation, or chemoembolization, systemic chemotherapy is utilized without proven effect. The combination of statins and standard chemotherapeutic agents in vitro is lacking. Methods: We exposed two hepatoma cell lines, HepG2 and PLC/PRF/5, to varying concentrations of the statins: fluvastatin, lovastatin, pravastatin, and simvastatin. We also evaluated the effect of cisplatin at 10μM with lovastatin and simvastatin at 5 and 10μM. Cells were collected at 48 hours for flow cytometry analysis. Proliferation assay was used to evaluate the effects of tamoxifen and statins in biologically attainable concentrations to examine if any synergistic effect exists with these agents. Student's t-test and analysis of variance calculations were performed to validate the results from the proliferation assays. Results: All statins inhibited proliferation of the two hepatoma cell lines; however pravastatin had the weakest effect of the four consistently. At 72 hours, lovastatin at 5 μM inhibited proliferation to 76% of control in the HepG2 cells at 75% in the PLC/PRF/5 cells. Cisplatin alone at 10 μM inhibited to 47% and 75% respectively. The combination of lovastatin 5 μM with cisplatin 10 μM inhibited to 41% and 59% respectively. Lovastatin 10 μM with cisplastin 10 μM inhibited to 37% and 54%, whereas lovastatin alone at this concentration inhibited to 67% and 66% in the HepG2 and PLC/PRF/5 cell lines. Simvastatin 5 μM inhibited proliferation to 73% of control in the HepG2 and 69% in the PLC/PRF/5 cells. Combined with cisplatin 10 μM, the HepG2 cells were inhibited to 39% and 49%. Flow cytometry data suggests that the primary effect of anti-proliferation was not apoptosis but increased cell numbers in the G0/G1 stage of the cell cycle. Interestingly, despite tamoxifen and the various statins having a strong anti-proliferative effect on these cells independently, their anti-proliferative properties were negated when combined in assays for both cell lines. Conclusion: Statins continue to offer promise as a beneficial adjuvant therapy to standard treatment HCC regimens. Further testing should be performed in prospective trials to confirm a survival benefit, and based on our in vitro data, it appears that trials utilizing statins other than pravastatin would show an even greater survival improvement.
Standardisation of the description of patellofemoral motion and comparison between different techniques (2002)

Patellofemoral motion is significant clinically, yet in the literature many different methods and terminologies are used, thus making comparison between studies difficult. We review and explain the different methods used for the description of patellofemoral joint motion, compare these methods by experimentation, and propose a standardised method. We found three main methods for describing patellar motion: motion of the patella about femoral body fixed axes, about patellar body fixed axes, and a combination of these. Description about femoral body fixed axes does not make sense clinically. Description about patellar body fixed axes is straightforward, yet the definition of these axes is prone to error due to the lack of anatomical landmarks. The combination method makes most sense clinically and uses more easily found anatomical landmarks. Patellar flexion varied by up to 26% when describing the motion about different axes. Tilt and shift were highly sensitive to the choices of coordinate systems and the axes of motion. The pattern of rotation was consistent between all methods; however, differences between the methods increased with patellar flexion. We propose the description of patello-femoral motion in terms of shift (along a femoral medial-lateral axis), tilt (about the patellar long axis), rotation (about a floating patellar anterior-posterior axis) and flexion (about the femoral medial-lateral axis).
PD21-07 URINARY MONOCYTE CHEMOATTRACTANT PROTEIN-1 (MCP-1/CCL2) EXPRESSION AS AN INDICATOR OF GLOBAL RESPONSE ASSESSMENT IN CLINICAL TRIAL FOR UNDERACTIVE BLADDER (UAB) (2020)

INTRODUCTION AND OBJECTIVE: UAB affects >15% of the population, is unrecognized, and has a major influence on the health and independence of many Americans. Last year we reported the 12-month interim results from a prospective open label study of intradetrusor injected adult muscle-derived cells (AMDC) treatment for UAB (NCT02463448) as well as possible companion biomarkers to evaluate patient response. Here we show that urinary Monocyte chemoattractant protein-1 (MCP-1/CCL2) expression corresponds with patient-reported Global Response Assessment (GRA) scores. METHODS: After IRB approval urine samples were collected from subjects with non-neurogenic UAB without outlet obstruction treated with 250 million AMDCs via 30 intradetrusor injections. Subjects were seen, assessed for adverse events and efficacy, and urine for biomarker testing was collected at 1, 3, 6 & 12-month post-injection. Levels of cytokines in the urine were measured using Luminex Multiplex assay (Millipore). Patients also reported a GRA on a scale of 1 to 7, where 1 is markedly worse (failure of therapy) and 7 is markedly improved (successful therapy). RESULTS: No serious procedure- or treatment-related AEs occurred. All patients reported similar levels of urinary MCP-1 at baseline. The patients that reported a GRA of 6-7 (moderately improved to markedly improved) 6 months post-injection had increased urinary expression of MCP-1; patients that reported a GRA of 4 (unchanged) had no appreciable increase in MCP-1. No patients reported a GRA of 1-3 (markedly worse to slightly worse). At 12 months post-injection, MCP-1 levels of GRA 6-7 remained higher than GRA of 4. CONCLUSIONS: MCP-1 is one of the key chemokines known to play an important role in wound healing and collagen synthesis. Elevated urinary expression of MCP-1 was observed in patients reporting moderately improved to markedly improved symptoms as a result of ADMC injections to treat UAB whereas patients that reported no improvement were observed not to have an increased in urinary MCP-1. Urinary MCP-1 might be of interest to future studies as a possible indicator of biological response in patients responding to ADMC therapy.Source of Funding: Robert B. & Ann Aikens Center for Neurourology Research
Evaluation of Calcium - Arsenazo III Stoichiometry in the Presence and Absence of Egta at Various pH (1985)

Abstract The calcium titration experiments were performed with 9 uM and 100 uM concentrations of Arsenazo III (Az) at various pH°s in the presence as well as in the absence of ethylene glycol-bis-(2-amino ethylether)-N,N°-tetra-acetic acid (EGTA). Analysis of results indicated that in the presence of EGTA in 9 uM Az solutions the Ca-Az stoichiometry changes from 1:1 to two Ca binding with one Az (2:1) upon increase in pH from 6.55 to 7.26. In the same systems but without EGTA the binding stoichiometry remains 1:1 throughout the pH range 6.55 to 7.32. In case of 100 uM Az III, however, EGTA has no effect on Ca-Az stoichiometry and it is 1:1 in the above pH range. An estimate of dissociation constants (KD) in EGTA containing systems showed a simultaneous decrease in KD by three orders of magnitude with a change in stoichiometry from 1:1 to 2:1 in the case of 9 uM Az III. At 100 uM dye concentration, with no change in 1:1 binding stoichiometry, KD also remained constant throughout the pH range studied. We suggest that at lower dye concentration a competition between Ca-Az and Ca-EGTA binding causes this change in stoichiometry. The discrepancy among the previous reports about Ca-Az stoichiometry appeared to be due to change in pH in the presence of EGTA.
Streptococcus pneumoniae Pilus Subunits Protect Mice against Lethal Challenge (2006)

Streptococcus pneumoniae is a major public health threat worldwide. The recent discovery that this pathogen possesses pili led us to investigate their protective abilities in a mouse model of intraperitoneal infection. Both active and passive immunization with recombinant pilus subunits afforded protection against lethal challenge with the S. pneumoniae serotype 4 strain TIGR4.
ChemInform Abstract: Chemistry, Catalysts, and Processes for Isoparaffin‐Olefin Alkylation: Actual Situation and Future Trends (1994)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Self-microemulsifying drug delivery system and nanoemulsion for enhancing aqueous miscibility of Alpinia galanga oil (2017)

Alpinia galanga oil (AGO) possesses various activities but low aqueous solubility limits its application particularly in aquatic animals. AGO has powerful activity on fish anesthesia. Ethanol used for enhancing water miscible of AGO always shows severe side effects on fish. The present study explores the development of self-microemulsifying drug delivery systems (SMEDDS) and nanoemulsions (NE) to deliver AGO for fish anesthesia with less or no alcohol. Pseudoternary phase diagrams were constructed to identify the best SMEDDS-AGO formulation, whereas NE-AGO were developed by means of high-energy emulsification. The mean droplet size of the best SMEDDS-AGO was 82 ± 0.5 nm whereas that of NE-AGO was 48 ± 1.6 nm. The anesthetic effect of the developed SMEDDS-AGO and NE-AGO in koi (Cyprinus carpio) was evaluated and compared with AGO ethanolic solution (EtOH-AGO). It was found that the time of induction the fish to reach the surgical stage of anesthesia was dose dependent. NE-AGO showed significantly higher activity than SMEDDS-AGO and EtOH-AGO, respectively. EtOH-AGO caused unwanted hyperactivity in the fish. This side effect did not occur in the fish anesthetized with SMEDDS-AGO and NE-AGO. In conclusion, SMEDDS and NE are promising delivery systems for AGO.
Selective Ring Opening Allene Metathesis: Polymerization or Ruthenium Vinylidene Formation (2021)

Selective ring-opening allene metathesis polymerization (ROAlMP) and ruthenium vinylidene formation from 1,2-cyclononadiene (1) by simple catalyst selection are discussed. Grubbs second generation catalyst (G2) favors the formation of an alkylidene leading to the ROAlMP of (1). Grubbs first generation catalyst (G1) favors vinylidene formation and prevents the homopolymerization of (1) even at elevated temperatures. Isolation and characterization of poly(1) by NMR analysis and MALDI-TOF confirms the generation of a well-defined polyallene that exhibits good thermal stability (TD ca. 400 ºC) and fluorescent properties. Ring-opening metathesis polymerization (ROMP) of a high strained norbornene derivative (NBE-iPr) at 80 ºC using the ruthenium vinylidene generated from (1) is also investigated. The discovery of ROAlMP allows for the simple accesses of well-defined polyallenes from commercially available catalysts and will ultimately guide structure-property determinations of polyallenes.
Capacitive Biosensing of Bacterial Cells: Sensitivity Optimization (2015)

Capacitive biosensors are promising tools toward detection of bacterial cells. However, their sensitivity remains insufficient compared with more conventional techniques. To understand how to optimize it, we propose a complete and quantitative analysis of the sensitivity of capacitive biosensors using a previously developed 2-D numerical simulator based on Poisson-Nernst-Planck equations. All key parameters of the electrolyte, bacterial cell, and electrode design are investigated, studied, and optimized. For each, an analytical expression relating the sensitivity to the parameter of interest is given. The guidelines and design tools, provided throughout this paper for the designer of capacitive biosensors, are eventually demonstrated by improving the sensitivity of our device under test by a factor of 4. Discussions based on experimental values and analytical models are provided throughout this paper.
Rapid fabrication of vascularized and innervated cell-laden bone models with biomimetic intrafibrillar collagen mineralization (2019)

Bone tissue, by definition, is an organic-inorganic nanocomposite, where metabolically active cells are embedded within a matrix that is heavily calcified on the nanoscale. Currently, there are no strategies that replicate these definitive characteristics of bone tissue. Here we describe a biomimetic approach where a supersaturated calcium and phosphate medium is used in combination with a non-collagenous protein analog to direct the deposition of nanoscale apatite, both in the intra- and extrafibrillar spaces of collagen embedded with osteoprogenitor, vascular, and neural cells. This process enables engineering of bone models replicating the key hallmarks of the bone cellular and extracellular microenvironment, including its protein-guided biomineralization, nanostructure, vasculature, innervation, inherent osteoinductive properties (without exogenous supplements), and cell-homing effects on bone-targeting diseases, such as prostate cancer. Ultimately, this approach enables fabrication of bone-like tissue models with high levels of biomimicry that may have broad implications for disease modeling, drug discovery, and regenerative engineering.
Correlations between Synthesis and Performance of Fe-Based PGM-Free Catalysts in Acidic and Alkaline Media: Evolution of Surface Chemistry and Morphology (2019)

The effects of the synthesis steps of a platinum group metal-free (PGM-free) catalyst on the surface chemistry, morphology, and electrochemical activity in acidic and alkaline media toward the oxygen reduction reaction (ORR) were studied. Each step exhibits a positive impact on catalyst activity. In acid media, etching of the silica template is the major contributor to the enhancement of the half-wave potential, as the ORR active sites formed during the first pyrolysis become more accessible. Further processing steps result even in higher accessibility and utilization of the 4e– transfer sites. In alkaline media, the second pyrolysis is a critical step that favors the complete reduction of oxygen to water, as the peroxide production is significantly diminished. The large heterogeneity in the porosity at each synthesis step indicates that this parameter needs to be further studied to attain better control of the morphology of the PGM-free catalyst, as it is an important factor that contributes to the active site utilization. The acid etching and second pyrolysis increase the meso- and macroporosity. Understanding the effects of each of the synthesis steps on the chemical composition, morphology and ORR activity of the PGM-free catalyst provides the necessary feedback for the design of synthetic schemes that increase the catalysts’ performance.
Moving solid state cyan lasers beyond 20 mW (2004)

We report on cavity-enhanced second-harmonic generation of 488 nm radiation in a 5 mm long periodically poled KTiOPO4 (PPKTP) crystal pumped by the output of a single-mode 976 nm semiconductor external cavity laser. At a pump laser output power of 660 mW, a mode-matching efficiency into an enhancement cavity of 65 % was observed. A maximum power of 156 mW at 488 nm was generated in the enhancement cavity of which 130 mW was coupled out. Under these pump laser conditions an overall optical conversion efficiency of 20 % and an overall electrical to optical efficiency of 9 % was measured. Both the spatial and spectral properties of the 488 nm beam are of very high quality. Typically, a near-diffraction-limited beam with M<sup>2</sup><1.1 is produced with low astigmatism and little ellipticity.
Synthesis and structural characterization of a dimeric cyanomanganate (III) complex K_7[(CN)_5MnOMn(CN)_5]CN (1974)

The mixing of saturated aqueous solutions of KCN and KMnO_4 with subsequent evaporation of water results in the formation and crystallization of a gold brown compound containing potassium, manganese, oxygen and cyanide ions.
Links between complexity theory and constrained block coding (2002)

The goal of this paper is to establish links between computational complexity theory and the theory and practice of constrained block coding. The complexities of several fundamental problems in constrained block coding are shown to be complete in various classes of the existing complexity-theoretic structure. The results include (relatively rare) /spl Sigma//sub 2//sup p/-, /spl Sigma//sub 3//sup p/, and NP/sup PP/-completeness results. Two types of problems are considered: (1) the problem of designing encoder and decoder circuits using minimum or approximately minimum hardware for a given constraint and a given rate; (2) computing the maximum rate of a block code for a given constraint and codeword length. In both cases, a constraint is specified by a deterministic finite state transition diagram. Another question studied is whether maximum-rate block codes can always be implemented by encoders and decoders of polynomial size. The answer to this question is shown to be closely related to the complexity of PP.
Freeze‐Align and Heat‐Fuse: Microwires and Networks from Nanoparticle Suspensions (2008)

Bildet eine Kette! Wässrige Kolloide mit Partikeldurchmessern zwischen 10 und 500 nm bilden beim Einfrieren und Gefriertrocknen ausgerichtete Mikrodrähte, die sich beim Tempern unter Beibehaltung der Morphologie zusammenlagern. Die Oberflächenladung lässt sich dabei so einstellen, dass der Ansatz auf Metalle, Metalloxide und organische Polymere anwendbar wird. Das Bild zeigt einen einzelnen Mikrodraht, der aus einem Polystyrolkolloid erhalten wurde. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2001/2008/z705512_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
The Regional Extent of Suppression: Strabismics Versus Nonstrabismics (2013)

Suppression extends throughout the central visual field in both strabismic and anisometropic forms of amblyopia. The strongest suppression occurs within the region of the visual field corresponding to the fovea of the fixing eye.
System-scenario-based design of dynamic embedded systems (2009)

In the past decade, real-time embedded systems have become much more complex due to the introduction of a lot of new functionality in one application, and due to running multiple applications concurrently. This increases the dynamic nature of today's applications and systems, and tightens the requirements for their constraints in terms of deadlines and energy consumption. State-of-the-art design methodologies try to cope with these novel issues by identifying several most used cases and dealing with them separately, reducing the newly introduced complexity. This article presents a generic and systematic design-time/run-time methodology for handling the dynamic nature of modern embedded systems, which can be utilized by existing design methodologies to increase their efficiency. It is based on the concept of system scenarios , which group system behaviors that are similar from a multidimensional cost perspective—such as resource requirements, delay, and energy consumption—in such a way that the system can be configured to exploit this cost similarity. At design-time, these scenarios are individually optimized. Mechanisms for predicting the current scenario at run-time, and for switching between scenarios, are also derived. This design trajectory is augmented with a run-time calibration mechanism, which allows the system to learn on-the-fly during its execution, and to adapt itself to the current input stimuli, by extending the scenario set, changing the scenario definitions, and both the prediction and switching mechanisms. To show the generality of our methodology, we show how it has been applied on four very different real-life design problems. In all presented case studies, substantial energy reductions were obtained by exploiting scenarios.