gebhart/patrepeval huggingface dataset:
Identification of a cDNA encoding an active asparaginyl endopeptidase of <i>Schistosoma mansoni</i> and its expression in <i>Pichia pastoris</i><sup>1</sup> (2000)

Asparaginyl endopeptidases, or legumains, are a recently identified family of cysteine-class endopeptidases. A single gene encoding a Schistosoma mansoni asparaginyl endopeptidase (a.k.a. Sm32 or schistosome legumain) has been reported, but by sequence homology it would be expected to yield an inactive product as the active site C197 had been replaced by N. We now describe a new S. mansoni gene in which C197 is present. Both gene products were expressed in Pichia pastoris. Autocatalytic processing to fully active C197 Sm32 occurred at acid pH. In contrast, N197 Sm32 was not processed and this is consistent with the hypothesis that C197 is essential for catalysis. This was confirmed by mutation of N197 to C and re-expression in Pichia. The availability of recombinant active Sm32 allows detailed analysis of its catalytic mechanism and its function(s) in the biology of this important human parasite.
Diagnostic role of head-up tilt test in patients with cough syncope (2016)

Syncope during coughing is a result of hypotension, rather than bradycardia. Coughing during HUT is a useful test in patients suspected to have cough syncope but in whom the history is not conclusive.
Parametric Study of State-of-Charge for an Electric Aircraft in Urban Air Mobility (2021)

View Video Presentation: https://doi.org/10.2514/6.2021-3181.vid A parametric study of the state of charge is performed using two separate battery models, i.e., energy-based and electrochemical-based battery models for a NASA-proposed conceptual multirotor aircraft in the urban environment. The parametric study is performed using an optimal control framework. The parameters considered for the parametric analysis are cruise airspeed, cruise altitude, climb profile, range, and required time of arrival under uncertainties (wind and departure-time). The key results suggest: i) the minimum-energy cruise airspeed (maximum final state of charge) increases with an increase in cruise altitude, ii) the minimum-energy cruise airspeed is independent of range; iii) design a cruise corridor for urban air mobility air traffic at the lowest possible altitude considering eddies/vortices from skyscrapers, other types of air traffic, and vertical obstacles, and iv) wind and departure-time uncertainties could adversely impact the predictability of the usage of the onboard lithium-ion polymer battery pack for an aircraft flying to meet the assigned required time of arrival in the urban air mobility environment. The state of charge results showed high dependence on the battery model. Therefore, this study demonstrates the need to have a validated battery model for the onboard lithium-ion polymer battery pack to predict the state of charge accurately.
Time-Modulation of High density Plasmas for Advanced Dry Etching Processes (2001)

International audience
Mapping a stable solvent structure landscape for aprotic Li–air battery organic electrolytes (2017)

Electrolyte instability is one of the greatest impediments that must be overcome for the practical development of rechargeable aprotic Li–air batteries.
Concentration effects on charge transport in dye-doped polymer light-emitting diodes (1998)

Light emitting diodes made of a single spin-coated layer of poly(9-vinylcarbazole) doped with coumarin-515 dye have been studied. The influence of dye concentration on emission and electrical characteristics is evidenced. Two different regimes are identified. At low concentrations, apparent hole injection barrier raises, holes are trapped and mobility decreases. External quantum efficiency increases with concentration. At concentrations larger than 10%wt, coumarin aggregates, photoluminescence yield drops and electroluminescence yield increases up to 0.1%-photons per electron. We show that using coumarin dye in a single-layer diode improves electron-hole injection and recombination balance more than using an additional hole-blocking layer.
An Amperometric Enzyme Biosensor Fabricated from Polyaniline Nanoparticles (2005)

Abstract The biosensor described here uses a novel aqueous‐based nanoparticulate polyaniline (PANI), doped with dodecylbenzenesulfonic acid (DBSA). The nanoparticles were applied to a glassy carbon electrode surface by electrodeposition techniques, and horseradish peroxidase (HRP) was subsequently electrostatically adsorbed to the nanoparticle‐modified surface. This biosensor format was demonstrated for H 2 O 2 sensing. Electrodeposited polymer nanoparticles resulted in highly ordered conductive nanostructured films, which were examined by scanning electron microscopy (SEM), atomic force microscopy (AFM), profilometry and spectroelectrochemistry. The surface of the films were characterized by a uniform array of nanoparticulate PANI (nanoPANI/DBSA) nodules and were shown to have a thickness of 350 nm. Physical techniques have shown that the nanofilms possess properties which allow for uniform electrostatic adsorption of protein to take place. This effective biosensor format, exhibits higher signal‐to‐background ratios and shorter response times than previous PANI biosensor configurations.
Safety and immunogenicity of the M72/AS01 candidate tuberculosis vaccine in HIV-infected adults on combination antiretroviral therapy (2014)

M72/AS01 was clinically well tolerated and immunogenic in this population, supporting further clinical evaluation in HIV-infected individuals in TB-endemic settings.
Assessment of clinical outcomes in patients with fibromyalgia: Analysis from the UK Medical Cannabis Registry (2023)

CBMP treatment was associated with improvements in fibromyalgia-specific symptoms, in addition to sleep, anxiety, and health-related quality of life. Those who reported prior cannabis use appeared to have a greater response. CBMPs were generally well-tolerated. These results must be interpreted within the limitations of study design.
Antibody Profiling and In Silico Functional Analysis of Differentially Reactive Antibody Signatures of Glioblastomas and Meningiomas (2023)

Studies on tumor-associated antigens in brain tumors are sparse. There is scope for enhancing our understanding of molecular pathology, in order to improve on existing forms, and discover new forms, of treatment, which could be particularly relevant to immuno-oncological strategies. To elucidate immunological differences, and to provide another level of biological information, we performed antibody profiling, based on a high-density protein array (containing 8173 human transcripts), using IgG isolated from the sera of <i>n</i> = 12 preoperative and <i>n</i> = 16 postoperative glioblastomas, <i>n</i> = 26 preoperative and <i>n</i> = 29 postoperative meningiomas, and <i>n</i> = 27 healthy, cancer-free controls. Differentially reactive antigens were compared to gene expression data from an alternate public GBM data set from OncoDB, and were analyzed using the Reactome pathway browser. Protein array analysis identified approximately 350-800 differentially reactive antigens, and revealed different antigen profiles in the glioblastomas and meningiomas, with approximately 20-30%-similar and 10-15%-similar antigens in preoperative and postoperative sera, respectively. Seroreactivity did not correlate with OncoDB-derived gene expression. Antigens in the preoperative glioblastoma sera were enriched for signaling pathways, such as <i>signaling by Rho-GTPases, COPI-mediated anterograde transport and vesicle-mediated transport,</i> while the <i>infectious disease, SRP-dependent membrane targeting cotranslational proteins</i> were enriched in the meningiomas. The pre-vs. postoperative seroreactivity in the glioblastomas was enriched for antigens, e.g., <i>platelet degranulation</i> and <i>metabolism of</i> lipid pathways; in the meningiomas, the antigens were <i>enriched in infectious diseases, metabolism of amino acids and derivatives,</i> and cell cycle. Antibody profiling in both tumor entities elucidated several hundred antigens and characteristic signaling pathways that may provide new insights into molecular pathology and may be of interest for the development of new treatment strategies.
Characterization of Edible Coatings and Microorganisms on Food Surfaces Using Fourier Transform Infrared Photoacoustic Spectroscopy (2001)

Fourier transform infrared photoacoustic spectroscopy (FT-IR/PAS) was used to study edible coatings and microorganisms on fruit surfaces (apple and honeydew melon). In the experiment, pregelatinized starch and whey protein concentrate were used to prepare edible coatings, while Saccharomyces cerevisiae, Lactobacillus casei, Escherchia coli, and Staphylococcus aureus were used for microbial study. The spectra obtained from the edible coatings on the apple surface indicate that the FT-IR/PAS technique can effectively distinguish the coating surface and the food substrate by examination of magnitude, phase angle, and generalized two-dimensional (G2D) correlation spectra. Results reported show the first application of FT-IR/PAS to directly differentiate a fruit surface (apple and honeydew melon) with/without microorganisms without elaborate sample preparation. The results demonstrate the potential of FT-IR/PAS to characterize the presence of microorganisms on fruit surfaces.
Mapping Systematic Reviews on Forensic Psychiatric Care: A Systematic Review Identifying Knowledge Gaps (2018)

<b>Background:</b> Forensic psychiatric care treats mentally disordered offenders who suffer mainly from psychotic disorders, although comorbidities such as personality disorders, neurodevelopmental disorders, and substance abuse are common. A large proportion of these patients have committed violent crimes. Their care is involuntary, and their caregivers' mission is complex: not only to rehabilitate the patient, but also to consider their risk for reoffending and their risk to society. The objective of this overview of systematic reviews is to identify, appraise, and summarize the existing knowledge in forensic psychiatric care and identify knowledge gaps that require further research. <b>Methods:</b> We undertook a systematic literature search for systematic reviews in five defined domains considered important in daily clinical practice within the forensic psychiatric care: (1) diagnostic assessment and risk assessments; (2) pharmacological treatment; (3) psychological interventions; (4) psychosocial interventions, rehabilitation, and habilitation; and (5) restraint interventions. The target population was mentally disordered offenders (forensic psychiatric patients aged >15 years). Each abstract and full text review was assessed by two of the authors. Relevant reviews then were assessed for bias, and those with moderate or low risk of bias were included. <b>Results:</b> Of 38 systematic reviews meeting the inclusion criteria, only four had a moderate risk of bias. Two aimed to incorporate as many aspects of forensic psychiatric care as possible, one investigated non-pharmacological interventions to reduce aggression in forensic psychiatric care, and one focused on women with intellectual disabilities in forensic care. However, most of the primary studies included in these reviews had high risks of bias, and therefore, no conclusions could be drawn. All of our identified domains must be considered knowledge gaps. <b>Conclusion:</b> We could not answer any of our research questions within the five domains because of the high risk of bias in the primary studies in the included systematic reviews. There is an urgent need for more research on forensic psychiatric care since all of our studied domains were considered knowledge gaps.
Sustainability and Resiliency Implications of Hurricane Sandy (2014)

Hurricane Sandy provides a unique opportunity to study sustainability and resiliency issues related to a major weather event. The material presented in this article is based on the Geo-Engineering Extreme Events Reconnaissance (GEER) Association, Report No. GEER-032, Version 1.
Validation of a new spectrometer for noninvasive measurement of cardiac output (2004)

Acetylene is a blood-soluble gas and for many years its uptake rate during rebreathing tests has been used to calculate the flow rate of blood through the lungs (normally equal to cardiac output) as well as the volume of lung tissue. A new, portable, noninvasive instrument for cardiac output determination using the acetylene uptake method is described. The analyzer relies on nondispersive IR absorption spectroscopy as its principle of operation and is configured for extractive (side-stream) sampling. The instrument affords exceptionally fast (30 ms, 10%–90%, 90%–10%, at 500 mL min−1 flow rates), interference-free, simultaneous measurement of acetylene, sulfur hexafluoride (an insoluble reference gas used in the cardiac output calculation), and carbon dioxide (to determine alveolar ventilation), with good (typically ±2% full-scale) signal-to-noise ratios. Comparison tests with a mass spectrometer using serially diluted calibration gas samples gave excellent (R2&amp;gt;0.99) correlation for all three gases, validating the IR system’s linearity and accuracy. A similar level of agreement between the devices also was observed during human subject C2H2 uptake tests (at rest and under incremental levels of exercise), with the instruments sampling a common extracted gas stream. Cardiac output measurements by both instruments were statistically equivalent from rest to 90% of maximal oxygen consumption; the physiological validity of the measurements was confirmed by the expected linear relationship between cardiac output and oxygen consumption, with both the slope and intercept in the published range. These results indicate that the portable, low-cost, rugged prototype analyzer discussed here is suitable for measuring cardiac output noninvasively in a point-of-care setting.
Immunopathologic changes in the thymus during the acute stage of experimentally induced feline immunodeficiency virus infection in juvenile cats (1997)

The feline thymus is a target organ and site of viral replication during the acute stage of feline immunodeficiency virus (FIV) infection. This was demonstrated by histologic, immunohistologic, flow cytometric, and virologic tests. Thymic lesions developed after 28 days postinoculation (p.i.) and included thymitis, premature cortical involution, and medullary B-cell hyperplasia with germinal center formation and epithelial distortion. Alterations in thymocyte subsets also developed. Fewer CD4+ CD8- cells were detected at 28 days p.i., while an increase in CD4- CD8+ cells resulted in an inversion of the thymic CD4/CD8 ratio of single-positive cells, similar to events in peripheral blood. Provirus was present in all thymocyte subpopulations including cortical CD1(hi), CD1(lo), and B cells. The CD1(hi) thymocyte proviral burden increased markedly after 56 days p.i., coincident with the presence of infiltrating inflammatory cells. Increased levels of provirus in the CD1(lo) thymocyte subpopulation were detected prior to 56 days p.i. This was likely due to inclusion of infected infiltrating inflammatory cells which could not be differentiated from mature, medullary thymocytes. Proviral levels in B cells also increased from 70 days p.i. Morphologic alterations, productive viral infection, and altered thymocyte subpopulations suggest that thymic function is compromised, thus contributing to the inability of FIV-infected cats to replenish the peripheral T-cell pool.
In vivo photorelease of GABA in the mouse cortex (2011)

Electrical stimulation has been used for more than 100 years in neuroscientific and biomedical research as a powerful tool for controlled perturbations of neural activity. Despite quickly driving neuronal activity, this technique presents some important limitations, such as the impossibility to activate or deactivate specific neuronal populations within a single stimulation site. This problem can be avoided by pharmacological methods based on the administration of receptor ligands able to cause specific changes in neuronal activity. However, intracerebral injections of neuroactive molecules inherently confound the dynamics of drug diffusion with receptor activation. Caged compounds have been proposed to circumvent this problem, for spatially and temporally controlled release of molecules. Caged compounds consist of a protecting group and a ligand made inactive by the bond between the two parts. By breaking this bond with light of an appropriate wavelength, the ligand recovers its activity within milliseconds. To test these compounds in vivo, we recorded local field potentials (LFPs) from the cerebral cortex of anesthetized female mice (CF1, 60-70 days, 20-30 g) before and after infusion with caged γ-amino-butyric-acid (GABA). After 30 min, we irradiated the cortical surface with pulses of blue light in order to photorelease the caged GABA and measure its effect on global brain activity. Laser pulses significantly and consistently decreased LFP power in four different frequency bands with a precision of few milliseconds (P < 0.000001); however, the inhibitory effects lasted several minutes (P < 0.0043). The technical difficulties and limitations of neurotransmitter photorelease are presented, and perspectives for future in vivo applications of the method are discussed.
Structural studies on vertebrate and invertebrate acetylcholinesterases and their complexes with functional ligands (2000)

The structure of Torpedo californica acetylcholinesterase (AChE) was examined in complex with several inhibitors which are either in use or under development for treatment of Alzheimer's disease. The inhibitors vary greatly in their structures, offering differing starting points for future drug design. The structure of T. californica AChE is also compared to the recently solved structure of Drosophila melanogaster AChE, the first invertebrate enzyme to have its structure determined. The structure of D. melanogaster AChE complexed with a potential insecticide is described. Drug Dev. Res. 50:573–583, 2000. © 2000 Wiley-Liss, Inc.
ChemInform Abstract: Synthesis and Characterization of Chemically Modified Hyaluronan and Chondroitin Sulfate (2011)

Abstract Review: 100 refs.
A Tandem Michael Addition Ring-Closure Route to the Metabotropic Receptor Ligand α-(Hydroxymethyl)glutamic Acid and Its γ-Alkylated Derivatives (2001)

ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTA Tandem Michael Addition Ring-Closure Route to the Metabotropic Receptor Ligand α-(Hydroxymethyl)glutamic Acid and Its γ-Alkylated DerivativesJiazhong Zhang, Judith L. Flippen-Anderson, and Alan P. KozikowskiView Author Information Georgetown University Medical Center, Drug Discovery Program, 3970 Reservoir Road NW, Washington, D.C. 20007-2197, and Laboratory for the Structure of Matter, Code 6030, Naval Research Laboratory, 4555 Overlook Avenue SW, Washington, D.C. 20375-5000 [email protected]Cite this: J. Org. Chem. 2001, 66, 22, 7555–7559Publication Date (Web):October 5, 2001Publication History Received18 June 2001Published online5 October 2001Published inissue 1 November 2001https://pubs.acs.org/doi/10.1021/jo010626nhttps://doi.org/10.1021/jo010626nbrief-reportACS PublicationsCopyright © 2001 American Chemical SocietyRequest reuse permissionsArticle Views525Altmetric-Citations29LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information SUBJECTS:Anions,Monomers,Organic compounds,Peptides and proteins,Post-translational modification Get e-Alerts
Application of the Statin-Associated Muscle Symptoms-Clinical Index to a Randomized Trial on Statin Myopathy (2017)

Evidence suggests 10% to 25% of patients experience statin-associated muscle symptoms (SAMS) [(1)][1], which may result in poor adherence and a greater incidence of cardiovascular events and costs [(2,3)][2]. Although there are no uniformly accepted diagnostic criteria for SAMS, the SAMS Clinical
Selective activation of angiotensin<scp>AT</scp><sub>2</sub>receptors attenuates progression of pulmonary hypertension and inhibits cardiopulmonary fibrosis (2014)

Taken together, our results suggest that the AT2 receptor agonist, C21, may hold promise for patients with PH.
Video translation system using face tracking and lip synchronization (2001)

We introduce a multi-modal English-to-Japanese and Japanese-to-English translation system that also translates the speaker's speech motion while synchronizing it to the translated speech. To retain the speaker's facial expression, we substitute only the speech organ's image with the synthesized one, which is made by a three-dimensional wire-frame model that is adaptable to any speaker. Our approach enables image synthesis and translation with an extremely small database. Also, we propose a method to track motion of the face from the video image. In this system, movement and rotation of the head is detected by template matching using a 3D personal face wire-frame model. By this technique, an automatic video translation can be achieved.