gebhart/patrepeval huggingface dataset:
Outage minimization with limited feedback for the fading relay channel (2006)

In this paper, we consider practical methods to approach the theoretical performance limits in the fading relay channel under different assumptions of transmitter channel knowledge. Specifically, we consider two degrees of transmitter channel knowledge: 1) perfect feedback is available and power control is employed and 2) no channel state knowledge is available at the transmitters and only spatial power allocation is possible. First, when perfect feedback is available, the optimal power control policy determines the ultimate limits of performance for constant rate transmission in the slow fading environment. However, in practice, perfect channel knowledge is not possible at the transmitters due to the finite capacity of the feedback links. We find practical methods to approach this performance limit through the use of power control with finite rate feedback. The finite-rate feedback results are shown for the low-complexity, full-diversity amplify-and-forward (AF) protocol. Interestingly, we see that only a few feedback bits are needed to achieve most of the gains of the optimal perfect feedback power control algorithm. Second, we consider the performance limit when the transmitters have no channel state knowledge and derive the optimal spatial power allocation between the source and relay for a given sum power constraint for the AF protocol. For most practical cases of interest, equal power allocation between the source and relay is shown to be nearly optimal. Our work suggests that there is minimal power savings from using spatial power allocation at the transmitters. To obtain large performance improvements over constant power transmission, it is imperative to have feedback for each realization of the channel state to allow for temporal power control.
Adenovirus-Based Vaccine with Epitopes Incorporated in Novel Fiber Sites to Induce Protective Immunity against Pseudomonas aeruginosa (2013)

Adenovirus (Ad) vector-based vaccines displaying pathogen-derived epitopes on Ad capsid proteins can elicit anti-pathogen immunity. This approach seems to be particularly efficient with epitopes incorporated into the Ad fiber protein. Here, we explore epitope insertion into various sites of the Ad fiber to elicit epitope-specific immunity. Ad vectors expressing the 14-mer Pseudomonas aeruginosa immune-dominant outer membrane protein F (OprF) epitope 8 (Epi8) in five distinct sites of the Ad5 fiber, loops CD (AdZ.F(CD)Epi8), DE (AdZ.F(DE)Epi8), FG (AdZ.F(FG)Epi8), HI (AdZ.F(HI)Epi8) and C terminus (AdZ.F(CT)Epi8), or the hexon HVR5 loop (AdZ.HxEpi8) were compared in their capacity to elicit anti-P. aeruginosa immunity to AdOprF, an Ad expressing the entire OprF protein. Intramuscular immunization of BALB/c mice with AdZ.F(FG)Epi8 or AdZ.F(HI)Epi8 elicited higher anti-OprF humoral and cellular CD4 and CD8 responses as well as enhanced protection against respiratory infection with P. aeruginosa compared to immunization with AdZ.F(CD)Epi8, AdZ.F(DE)Epi8, AdZ.F(CT)Epi8 or AdZ.HxEpi8. Importantly, repeat administration of the fiber- and hexon-modified Ad vectors boosted the OprF-specific humoral immune response in contrast to immunization with AdOprF. Strikingly, following three doses of AdZ.F(FG)Epi8 or AdZ.F(HI)Epi8 anti-OprF immunity surpassed that induced by AdOprF. Furthermore, in the presence of anti-Ad5 immunity, immunization with AdZ.F(FG)Epi8 or AdZ.F(HI)Epi8, but not with AdOprF, induced protective immunity against P. aeruginosa. This suggests that incorporation of epitopes into distinct sites of the Ad fiber is a promising vaccine strategy.
Analysis of Reproducibility of Proteome Coverage and Quantitation Using Isobaric Mass Tags (iTRAQ and TMT) (2016)

This study aimed to compare the depth and reproducibility of total proteome and differentially expressed protein coverage in technical duplicates and triplicates using iTRAQ 4-plex, iTRAQ 8-plex, and TMT 6-plex reagents. The analysis was undertaken because comprehensive comparisons of isobaric mass tag reproducibility have not been widely reported in the literature. The highest number of proteins was identified with 4-plex, followed by 8-plex and then 6-plex reagents. Quantitative analyses revealed that more differentially expressed proteins were identified with 4-plex reagents than 8-plex reagents and 6-plex reagents. Replicate reproducibility was determined to be ≥69% for technical duplicates and ≥57% for technical triplicates. The results indicate that running an 8-plex or 6-plex experiment instead of a 4-plex experiment resulted in 26 or 39% fewer protein identifications, respectively. When 4-plex spectra were searched with three software tools-ProteinPilot, Mascot, and Proteome Discoverer-the highest number of protein identifications were obtained with Mascot. The analysis of negative controls demonstrated the importance of running experiments as replicates. Overall, this study demonstrates the advantages of using iTRAQ 4-plex reagents over iTRAQ 8-plex and TMT 6-plex reagents, provides estimates of technical duplicate and triplicate reproducibility, and emphasizes the value of running replicate samples.
Investigations of LSM-YSZ as Air Electrode Materials for Solid Oxide Fuel Cells (1999)

Composite air electrodes of 50/50 vol% LSM- YSZ where LSM =(0x0.5) were prepared by colloidal deposition technique. The electrodes were then examined by scanning electron microscopy (SEM) and studied by ac impedance spectroscopy in order to improve the performance of a solid oxide fuel cell (SOFC). Reproducible impedance spectra were confirmed by using the improved cell, consisting of LSM- YSZ/YSZ/LSM-YSZ. These spectra were a strong function of operating temperature and the stable conditions for the cells were typically reached at . The typical spectra measured for an air//air cell at were composed of two arcs. Addition of YSZ to the LSM electrode led to a pronounced decrease in cathodic resistivity of LSM-YSZ composite electrodes. Polishing the electrolyte surface to eliminate the influences of surface impurities could further reduce cathode resistivity. The cathodic resistivity of the LSM-YSZ electrodes with catalytic interlayer (Ni or Sr) was much smaller than that of LSM-YSZ electrodes without catalytic interlayer. In addition, the cathodic resistivity of the LSM-YSZ electrodes was a strong function of composition of electrode materials, the electrolyte geometry, and applied current.
Use of a chiA probe for detection of chitinase genes in bacteria from the Chesapeake Bay1 (2000)

PCR primers specific for the chiA gene were designed by alignment and selection of highly conserved regions of chiA sequences from Serratia marcescens, Alteromonas sp., Bacillus circulans and Aeromonas caviae. These primers were used to amplify a 225 bp fragment of the chiA gene from Vibrio harveyi to produce a chiA gene probe. The chiA PCR primers and probe were used to detect the presence of the chiA gene in an assemblage of 53 reference strains and gave consistent results. Selected chiA fragments amplified by PCR were cloned and sequenced from nine known strains and from Chesapeake Bay isolates 6d and 11d. This confirmed the specificity and utility of the primers for detection of chiA-positive environmental strains. Over 1000 bacterial isolates from Chesapeake Bay water samples were tested for the presence of the chiA gene which was found to be present in 5-41% (average 21%) of the culturable bacterial community. The approach developed in this study was valuable for isolation and enumeration of chiA-positive bacteria in environmental samples.
Germline and germline mosaic PTEN mutations associated with a Proteus-like syndrome of hemihypertrophy, lower limb asymmetry, arteriovenous malformations and lipomatosis (2000)

Germline PTEN mutations cause Cowden syndrome (CS) and Bannayan-Riley-Ruvalcaba syndrome (BRR), two hamartoma-tumour syndromes, and somatic PTEN alterations have been shown to participate, to a greater or lesser extent, in a wide variety of sporadic neoplasia. PTEN is a tumour suppressor and dual-specificity phosphatase which affects apoptosis via its lipid phosphatase activity in the phosphoinositol-3-kinase and AKT pathway as well as inhibiting cell spreading via the focal adhesion kinase pathway. CS and BRR share some features, such as hamartomas and lipomatosis. To determine whether other syndromes characterized by overgrowth and lipomas are part of the PTEN syndrome spectrum, we ascertained six individuals with overgrowth and lipomas but who did not meet the diagnostic criteria for CS or BRR. Five had Proteus syndrome and one, a Proteus-like syndrome. When germline DNA and DNA from at least one involved tissue per case were examined for PTEN mutations, only the Proteus-like patient was found to harbour a germline R335X mutation. Interestingly, a lipomatous mass, an epidermoid naevus and arteriovenous malformation tissue, all of which were sampled from physically distinct sites, were all found to carry a second hit R130X mutation on the allele opposite the germline R335X. Both mutations have been described in CS and BRR. We postulate that the second hit, R130X, occurred early in embryonic development and may even represent germline mosaicism. Thus, PTEN may be involved in Proteus-like syndrome with its implications for cancer development in the future.
Abstract 15491: Effects of Intensive Smoking Cessation Counseling After Acute Coronary Syndrome on 5-year Incidence of Major Adverse Cardiovascular Events and Smoking Abstinence (2020)

Introduction: Smoking cessation is recommanded after an acute coronary syndrome (ACS). Long-term cardiovascular benefits of smoking cessation counseling during a hospitalization and shortly after discharge for an ACS remains unknown. We aimed to study the 5-year incidence of major adverse cardiovascular events (MACE, defined as cardiovascular death, myocardial infarction, stroke, unplanned coronary revascularization or hospitalization due to angina) and smoking abstinence after ACS, comparing usual care to an intensive smoking cessation counseling intervention in hospital and after discharge. Methods: We studied 951 smokers from the SPUM-ACS cohort hospitalized for ACS between 2009 and 2014 in a multicenter prospective study with information available at the 5-year follow-up visit on incident MACE. At baseline in 4 study centers, a non-randomized part of smokers with ACS received proactive in-hospital smoking cessation counseling along with several telephone counseling over a 2 months period after discharge. We compared this intensive counseling group to the usual care group, regarding the incidence of MACE at 5 years. We used a multivariate adjusted Cox-proportional hazards model to estimate hazard ratios (HR) and 95% confidence intervals (CI). The secondary outcome was smoking cessation rate 5 years post-ACS. Results: Out of 951 smokers with ACS, 228 (35%) received the intensive in-hospital smoking intervention, of which 186 (82%) accepted to receive counseling after discharge. After multivariable adjustment for age, sex, education and study site, the 5-year risk of a recurrent MACE was comparable between the intensive counseling group and the usual care group, with a multivariable HR of 1.02 (95% CI 0.71-1.46, p=0.92). Among 669 patients alive 5 years post-ACS with known smoking status, 95 (56%) had stopped smoking in the intensive counseling group and 234 (47%) in the usual care group, with a multivariate odds ratio of 0.69 (95% CI 0.47-1.03; p=0.07). Conclusions: In this observational study, an intensive smoking cessation intervention during hospitalization for ACS with post-discharge telephone counseling did not reduce 5-year recurrence of MACE. However, this early intervention may confer a benefit for long-term smoking abstinence.
A Study of User Selection Method for Access Control Scheme on Multi-Beam Massive MIMO System (2018)

Multi-user multiple input multiple output (MU-MIMO) and massive MIMO transmission techniques can perform high-speed and simultaneous transmissions in wireless communications. However, transmission technologies need channel state information (CSI) feedback for channel estimation. CSI feedback decreases transmission efficiency owing to the overhead of access control for frame exchange. In related work, multi-beam massive MIMO transmission with CSI feedback elimination was proposed to resolve this issue. In this paper, we proposed an access control scheme for multibeam massive MIMO transmission called the overhead less access control scheme and studied a user selection method for fairness for terminals. We confirmed that the proposed access control with user selection scheme obtains a transmission efficiency of 90% or more.
EORTC 18991 phase III trial: Long-term adjuvant pegylated interferon-α2b (PEG-IFN) versus observation in resected stage III melanoma: Long-term results at 7.6-years follow-up. (2011)

8506b Background: EORTC 18991 is the largest adjuvant trial ever conducted in stage III melanoma. It assessed the efficacy and toxicity of long-term PEG-IFN vs Observation (Obs.). Methods: PEG-IFN (Induction at 6µg/Kg/wk, sc, 8 weeks; followed by Maintenance at 3µg/Kg/wk, sc) for a total treatment duration of up to 5 years was compared to Obs. in 1256 patients (pts) with stage III melanoma (anyTN1-2M0 without in-transit metastases). Randomization was stratified for nodal involvement N1 (microscopic) vs N2 (palpable nodes), # of nodes, Breslow and ulceration of primary, sex and center. Distant metastasis-free survival (DMFS) was the primary endpoint. Relapse-free survival (RFS) was the pre-specified regulatory primary endpoint. Overall survival (OS) was the secondary endpoint. Intent-to-treat analysis was performed Results: Median follow-up was 7.6 yrs is shown in the table. Conclusions: Long-term PEG-IFN therapy in stage III melanoma had a significant and sustained impact on RFS, but not on DMFS and OS. Pts with only microscopic nodal involvement (sentinel node positive) seemed to have a greater benefit of PEG-IFN in terms of RFS, and marginally regarding DMFS and OS. In the smaller subgroup of pts with sentinel node positive and with ulcerated melanoma, the benefit seemed to be the most striking and consistent regarding all endpoints, and was maintained at long term follow-up. These effects have been observed in 2 consecutive EORTC trials (18952 and 18991) involving 2,644 pts. RFS DMFS OS All pts (N=1,256) Obs. PEG-IFN Obs. PEG-IFN Obs. PEG-IFN Nb. events 406 384 375 370 336 332 7-year rates 35% 39% 40% 42% 46% 48% Median (yrs) 2.2 3.0 3.2 3.9 5.6 6.2 HR (95%CI) 0.87 (0.76-1.00) 0.93 (0.81-1.07) 0.96 (0.82-1.11) P value 0.05 0.33 0.57 N2 pts (N=713) HR (99%CI) 0.89 (0.71-1.13) 0.96 (0.76-1.22) 1.00 (0.78-1.29) P value 0.21 0.66 0.97 N1 pts (N=543) HR (99%CI) 0.82 (0.61-1.10) 0.86 (0.63-1.17) 0.86 (0.62-1.21) P value 0.08 0.22 0.26 N1 pts & ulc. (N=186) HR (99%CI) 0.72 (0.46-1.13) 0.65 (0.41-1.04) 0.59 (0.35-0.97) p-value 0.06 0.02 0.006 Abbreviations: HR, hazard ratio; CI, confidence interval; N1, microscopic (sentinel node positive); N2, palpable nodes; ulc., ulcerated melanoma.
Whole Body Vibration Levels: A Realistic Baseline for Standards (1976)

&lt;div class="htmlview paragraph"&gt;Statistical measures of whole-body vibration from ambulation are shown to be higher than those from operation of earthmoving machinery and significantly higher than published guidelines for human exposure to whole-body vibration. The inconsistency of human response to low level vibration of technological origin as compared to human imperceptiveness to high level vibration from ambulation is discussed.&lt;/div&gt;
Introduction for the Special Issue: Statistical Fluid Dynamics (2022)

Characterizing complex material consists in establishing the relationship between flow rheology during forming processes and the induced micro-structural state that affects directly the final mechanical properties of the formed parts [...].
Nonlinear Semigroups, Fixed Points, and Geometry of Domains in Banach Spaces (2005)

# Mappings in Metric and Normed Spaces # Differentiable and Holomorphic Mappings in Banach Spaces # Hyperbolic Metrics on Domains in Complex Banach Spaces # Some Fixed Point Principles # The Denjoy-Wolff Fixed Point Theory # Generation Theory for One-Parameter Semigroups # Flow-Invariance Conditions # Stationary Points of Continuous Semigroups # Asymptotic Behavior of Continuous Flows # Geometry of Domains in Banach Spaces
Bypassing the proline/thiazoline requirement of the macrocyclase PatG (2017)

Biocatalysis is a fast developing field in which an enzyme's natural capabilities are harnessed or engineered for synthetic chemistry. The enzyme PatG is an extremely promiscuous macrocyclase enzyme tolerating both non-natural amino acids and non-amino acids within the substrate. It does, however, require a proline or thiazoline at the C-terminal position of the core peptide which means the final product must contain this group. Here, we show guided by structural insight we have identified two synthetic routes, triazole and a double cysteine, that circumvent this requirement. With the triazole, we show PatGmac can macrocyclise substrates that do not contain any amino acids in the final product.
Characterisation of transition state structures for protein folding using 'high', 'medium' and 'low'  -values (2008)

It has been suggested that Phi-values, which allow structural information about transition states (TSs) for protein folding to be obtained, are most reliably interpreted when divided into three classes (high, medium and low). High Phi-values indicate almost completely folded regions in the TS, intermediate Phi-values regions with a detectable amount of structure and low Phi-values indicate mostly unstructured regions. To explore the extent to which this classification can be used to characterise in detail the structure of TSs for protein folding, we used Phi-values divided into these classes as restraints in molecular dynamics simulations. This type of procedure is related to that used in NMR spectroscopy to define the structure of native proteins from the measurement of inter-proton distances derived from nuclear Overhauser effects. We illustrate this approach by determining the TS ensembles of five proteins and by showing that the results are similar to those obtained by using as restraints the actual numerical Phi-values measured experimentally. Our results indicate that the simultaneous consideration of a set of low-resolution Phi-values can provide sufficient information for characterising the architecture of a TS for folding of a protein.
Highly Diverse Heparan Sulfate Analogue Libraries:  Providing Access to Expanded Areas of Sequence Space for Bioactivity Screening (2003)

Structurally diverse heparan sulfate analogue libraries were produced chemicoenzymatically from heparin. They possess vastly more heterogeneity than tissue heparan sulfates, expand the sequence space available for screening, and can help identify minimal structural features associated with activity. Library components are likely to exhibit fewer nonspecific interactions and side-effects than heparin or simple chemically modified heparin. A strategy for their use is illustrated for the fibroblast growth factor-receptor tyrosine kinase signaling system.
Malaria prevention: from immunological concepts to effective vaccines and protective antibodies (2018)

Development of a malaria vaccine remains a critical priority to decrease clinical disease and mortality and facilitate eradication. Accordingly, RTS,S, a protein-subunit vaccine, has completed phase III clinical trials and confers ~30% protection against clinical infection over 4 years. Whole-attenuated-sporozoite and viral-subunit vaccines induce between 20% and 100% protection against controlled human malaria infection, but there is limited published evidence to date for durable, high-level efficacy (>50%) against natural exposure. Importantly, fundamental scientific advances related to the potency, durability, breadth and location of immune responses will be required for improving vaccine efficacy with these and other vaccine approaches. In this Review, we focus on the current understanding of immunological mechanisms of protection from animal models and human vaccine studies, and on how these data should inform the development of next-generation vaccines. Furthermore, we introduce the concept of using passive immunization with monoclonal antibodies as a new approach to prevent and eliminate malaria.
High-resolution Patterning Technologies using Ink-jet Printing and Laser Processing for Organic TFT Array (2013)

A 150 ppi organic thin film transistor (OTFT) array, which TFT have pixel circuit with two transistors and one capacitor was fabricated by printing methods and laser processing. TFT array pitch was 169 &#x3BC;m, a minimum width of the source electrode was 15 &#x3BC;m, channel length was 5 &#x3BC;m, and a diameter of via hole was about 20 &#x3BC;m. We have developed the surface energy controlled ink-jet printing with UV irradiation for fine Ag electrodes and the conventional ink-jet printing for organic semiconductor (OSC), and laser processing for Via hole.
Coating Films of 20B <sub>2</sub> O <sub>3</sub> ·80SiO <sub>2</sub> by the Sol‐Gel Method (1987)

Alkali‐free borosilicate films of 20B 2 O 3 . 80SiO 2 (in mol%) have been prepared by the sol‐gel method. The transparency of these films was found to be very sensitive to the humidity of the atmosphere during the coating process. Transparent films were obtained only in a dry atmosphere. Measurements of IR spectra and load at scratch showed that these films were densified above 600°C.
Glycan characterization of the NIST RM monoclonal antibody using a total analytical solution: From sample preparation to data analysis (2017)

Glycosylation is an important attribute of biopharmaceutical products to monitor from development through production. However, glycosylation analysis has traditionally been a time-consuming process with long sample preparation protocols and manual interpretation of the data. To address the challenges associated with glycan analysis, we developed a streamlined analytical solution that covers the entire process from sample preparation to data analysis. In this communication, we describe the complete analytical solution that begins with a simplified and fast N-linked glycan sample preparation protocol that can be completed in less than 1 hr. The sample preparation includes labelling with RapiFluor-MS tag to improve both fluorescence (FLR) and mass spectral (MS) sensitivities. Following HILIC-UPLC/FLR/MS analyses, the data are processed and a library search based on glucose units has been included to expedite the task of structural assignment. We then applied this total analytical solution to characterize the glycosylation of the NIST Reference Material mAb 8761. For this glycoprotein, we confidently identified 35 N-linked glycans and all three major classes, high mannose, complex, and hybrid, were present. The majority of the glycans were neutral and fucosylated; glycans featuring N-glycolylneuraminic acid and those with two galactoses connected via an α1,3-linkage were also identified.
Anchoring of Rare-Earth-Based Single-Molecule Magnets on Single-Walled Carbon Nanotubes (2009)

A new heteroleptic bis(phthalocyaninato) terbium(III) complex 1, bearing a pyrenyl group, exhibits temperature and frequency dependence of ac magnetic susceptibility, typical of single-molecule magnets. The complex was successfully attached to single-walled carbon nanotubes (SWNTs) using pi-pi interactions, yielding a 1-SWNT conjugate. The supramolecular grafting of 1 to SWNTs was proven qualitatively and quantitatively by high-resolution transmission electron microscopy, emission spectroscopy, and atomic force spectroscopy. Giving a clear magnetic fingerprint, the anisotropy energy barrier and the magnetic relaxation time of the 1-SWNT conjugate are both increased in comparison with the pure crystalline compound 1, likely due to the suppression of intermolecular interactions. The obtained results propose the 1-SWNT conjugate as a promising constituent unit in magnetic single-molecule measurements using molecular spintronics devices.