MP8-05 OBESITY-INDUCED METABOLIC SYNDROME PROMOTE LOWER URINARY TRACT SYMPTOMS (LUTS) IN A MOUSE MODEL (2015)

You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Basic Research I1 Apr 2015MP8-05 OBESITY-INDUCED METABOLIC SYNDROME PROMOTE LOWER URINARY TRACT SYMPTOMS (LUTS) IN A MOUSE MODEL Qiqi He, Sanjeev Shukla, Zhiping Wang, Guiming Liu, Bernadette Erokwu, Chris Flask, Lan Lu, Melissa Babcook, Firouz Daneshgari, and Sanjay Gupta Qiqi HeQiqi He More articles by this author , Sanjeev ShuklaSanjeev Shukla More articles by this author , Zhiping WangZhiping Wang More articles by this author , Guiming LiuGuiming Liu More articles by this author , Bernadette ErokwuBernadette Erokwu More articles by this author , Chris FlaskChris Flask More articles by this author , Lan LuLan Lu More articles by this author , Melissa BabcookMelissa Babcook More articles by this author , Firouz DaneshgariFirouz Daneshgari More articles by this author , and Sanjay GuptaSanjay Gupta More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.272AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Obesity is a major risk factor involved in the development of metabolic syndrome (MS). Accumulating evidences suggests that obesity and MS contributes to lower urinary tract symptoms (LUTS) through alterations in the phenotype of bladder and prostate. The relationship between MS and LUTS has recently been evaluated in humans; however, there is lack of appropriate in vivo models to allow further exploration of those associations. Animal models may provide new insights into the molecular mechanisms of obesity-mediated LUTS to facilitate diagnosis and therapeutic interventions. METHODS Mice on C57BL/6N and B6.V-Lepob/J background were obtained from the Jackson Laboratory at 6 weeks of age and maintained on regular diet for 30 weeks. Animals were subjected to the assessment of body weight (BW), body length (BL), waist circumference (WC), body mass index (BMI), blood glucose (BG), plasma insulin (INS), plasma leptin (LEP), total cholesterol (CHO), free fatty acid (FFA) and evaluated for urinary voiding functions. Total body fat measurement, prostate and bladder volumes were analyzed by MRI followed by histological evaluation of the organs. These parameters were used to examine the associations between MS and LUTS. RESULTS Obesity parameters such as BW, WC, and BMI were significantly higher in B6.V-Lepob/J mice compared to C57BL/6N mice (p<0.01). Higher levels of total CHO and FFA were noted in B6.V-Lepob/J mice than C57BL/6N mice (p<0.05). These results were concurrent with frequency, lower average urine volume and other urinary voiding dysfunctions in B6.V-Lepob/J mice. MRI assessment demonstrate marked increase in body fat and prostate volume in these mice. Histology of prostate in B6.V-Lepob/J mice showed increased gland crowding and infiltration of immune cells in the stroma, compared to C57BL/6N mice. The regression and correlation analysis indicate that peritoneal fat (R=0.831; p<0.002), BG (R=0.712; p<0.01) and prostate volume (R=0.706; p<0.05) strongly correlate with LUTS whereas BMI, WC, BMI, INS, CHO and FFA moderately correlate with the prevalence of voiding dysfunctions. CONCLUSIONS Our findings suggests that LUTS may be attributable in part to obesity and MS. Further examination of our in vivo model may lead to understand the underlying pathophysiological mechanisms of obesity-induced LUTS in humans. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e74-e75 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.Metrics Author Information Qiqi He More articles by this author Sanjeev Shukla More articles by this author Zhiping Wang More articles by this author Guiming Liu More articles by this author Bernadette Erokwu More articles by this author Chris Flask More articles by this author Lan Lu More articles by this author Melissa Babcook More articles by this author Firouz Daneshgari More articles by this author Sanjay Gupta More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...