gebhart/patrepeval huggingface dataset:
Detection and purification of cytochromes P-450 in animal tissues with monoclonal antibodies. (1984)

Monoclonal antibody (MAb)-based radioimmunoassay (RIA) and immunopurification procedures were used to probe the immunochemical relatedness of cytochromes P-450 in tissues from different species. RIAs based on MAb 1-7-1 and MAb 1-31-2, both to the major 3-methylcholanthrene (MC)-induced rat liver microsomal cytochrome P-450, detected antigenically related forms of cytochrome P-450 in liver microsomes from MC-induced rats, C57BL/6 and DBA/2 mice, hamsters, and guinea pigs, and in lung and kidney microsomes of MC-induced rats. Individual cytochromes P-450 were also isolated from these microsomes by MAb-directed immunopurification. When bound to Sepharose, MAb 1-7-1 adsorbed two species of Mr 56,000 and 57,000 from liver microsomes from rats and C57BL/6 mice, while MAb 1-31-2 adsorbed only the Mr 57,000 polypeptide. These results reveal that livers from both rats and C57BL/6 mice contain a cytochrome P-450 (Mr 56,000) with the epitope for 1-7-1 and a cytochrome P-450 (Mr 57,000) with epitopes for both 1-31-2 and 1-7-1. Additional immunochemical relatedness between the cytochromes P-450 in different tissues was demonstrated by MAb-directed immunopurification of cytochromes P-450 from DBA/2 mouse liver (Mr 56,000), guinea pig liver (Mr 53,000), hamster liver (Mr 57,000), and rat lung (Mr 57,000). These results demonstrate the efficacy of MAb-based RIA and immunopurification procedures for simple and rapid detection and purification of cytochromes P-450 from a variety of tissues.
Characterization of Schottky Barrier Diodes on Heteroepitaxial Diamond on 3C-SiC/Si Substrates (2019)

We demonstrated and characterized Schottky barrier diodes (SBDs) fabricated on heteroepitaxial diamond films grown onto 3C-SiC/Si substrates. SBDs showed clear diode properties with rectification ratios above 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">9</sup> at ±5 V and maintained above 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">8</sup> even at 500 K. Temperature dependence of the Schottky barrier height (SBH) and the ideality factor was explained by assuming inhomogeneous Schottky barriers following the Gaussian distribution. For leakage current analysis, two types of defects, linear pits (LPs) and nonepitaxial crystals (NCs), were shown to be fatal defects, causing the flow of leakage currents. The leakage current was found to be an exponential function of the length of the LPs and a linear function of the size of the NCs. These defects can be suppressed by highly oriented heteroepitaxial diamond films with reduction of tilt and twist spread for diamond nuclei and optimized growth condition. Moreover, intrinsic layer thickening and inserting a buffer layer stopping propagation of dislocations are also effective. By reducing both crystal defects, we can obtain device properties comparable to SBDs fabricated on homoepitaxial diamond films because the leakage currents can be suppressed.
A ’Cognitive Driving Framework’ for Collision Avoidance in Autonomous Vehicles (2015)

The Cognitive Driving Framework is a novel method for forecasting the future states of a multi-agent system that takes into consideration both the intentions of the agents as well as their beliefs about the environment. This is partic-ularly useful for autonomous vehicles operating in an urban environment. The algorithm maintains a posterior probability distribution over agent intents and beliefs in order to more accurately forecast their future behavior. This allows an agent navigating the environment to recognize dangerous situations earlier and more accurately than competing algorithms, therefore allowing the agent take actions in order to prevent collisions. This paper presents the Cognitive Driving Framework in detail and describes its application to intersection navigation for au-tonomous vehicles. The effects of different parameter choices on the performance of the algorithm are analyzed and experiments are conducted demonstrating the ability of the algorithm to predict and prevent automobile collisions caused by human error in multiple intersection navigation scenarios. The results are compared to the performance of prevailing methods; namely reactionary planning and constant velocity forecasting.
Material Modeling of High-Pressure Compliant Syntactic Foams (2015)

Syntactic foam, specifically a host urethane embedded with hollow microspheres, has been shown to be an effective method to treat pressure pulsations, also known as noise, within a hydraulic system; however, the current generation of foam becomes less effective with increasing system pressure, particularly ineffective above 7 MPa. Material modeling predicts that increasing the initial internal pressure of a microsphere will allow voids within the foam to retain their size at pressures up to 35 MPa and the foam will remain compliant at those pressures. Noise is attenuated by an expansion chamber lined with syntactic foam when the system pressure causes embedded microspheres to collapse, which leaves a gaseous void within the host urethane, greatly reducing the effective bulk modulus of the foam. Predicted material properties are then used in conjunction with a previously developed linear acoustic model to predict the effectiveness of developmental syntactic foams. Changing the mechanical properties of the current host urethane does not have a drastic impact on the overall performance unless the properties have been reduced to the approximate order as the properties of the void. The factors with the most consequence on noise control effectiveness are the internal pressure of the microspheres and system pressure.
Abstract 250: Potential Plaque Stabilizing Effects of IGF-1 in Atherosclerosis (2006)

The role of insulin-like growth factor-1 (IGF-1) in atherosclerosis remains unclear. Whereas some studies report a potential anti-atherogenic effect of this hormone, others point towards an unfavor...
Immediate versus early (24-hours) urinary catheter removal after elective minimally invasive colonic resection: study protocol for a randomized, multicenter, non-inferiority trial (2022)

ClinicalTrials.gov NCT05249192. Prospectively registered on February 21, 2022.
A new dimerization reaction producing 2‐amino‐9‐oxopyrrolo‐[2,1‐<i>b</i>]quinazoline‐1‐carbonitriles and analogous pyrrolo‐[1,2‐<i>a</i>]thieno[3,2‐<i>d</i>] pyrimidinecar bonitriles (2001)

Abstract A series of 2‐alkylthio‐4‐oxo‐3‐quinazolineacetonitriles 4 and 2‐alkylthio‐4‐oxothieno[3,2‐ d ]pyrimidine‐3‐acetonitriles 8 was prepared. Upon treatment with sodium hydride, compounds 4 and 8 react to give 2‐amino‐4,9‐dihydro‐9‐oxopyrrolo[2,1‐ b ]quinazoline‐1‐carbonitriles 9 and 6‐amino‐4,9‐dihydro‐9‐oxopyrrolo[1,2‐ a ]thieno[3,2‐ d ]pyrimidine‐7‐carbonitriles 10 , respectively. The transformation of compounds 4 and 8 to the tricyclic aminonitriles 9 and 10 involves a dimerization step followed by a pyrrole cyclization. The tert ‐butyl ester derivatives 4d and 8d upon treatment with sodium hydride underwent a Thorpe‐Ziegler cyclization to provide enaminoesters of fused 1,3‐thiazines ( 16 and 17 , respectively) as major products.
Processing of ultrasonic measurements for nondestructive evaluation of critical optical components (2001)

Real-time nondestructive evaluation of critical optics in high-energy, pulsed laser experiments is crucial from a safety as well as maintenance viewpoint. Fluence levels in short pulse, high-energy lasers can produce pits and cracks in the surfaces of the lasers’ optical components. These flaws in the optical glass can adversely effect the production of the laser light or even result in a catastrophic failure of the optical component itself. Consequently, the identification and characterization of these flaws is critical. The production requirements of our target laser system mandate the automation of any inspection system. Consequently, it is necessary to develop an autonomous solution to process the reflected acoustic signals and, using this information, detect, localize, and characterize the optical flaws. In this paper we develop sophisticated signal and image processing techniques to automatically analyze laser-induced damage in optical glass. After preprocessing the raw channel measurement data from two orthogonal, narrow beamwidth, transducer arrays, a two-dimensional power image is created. A physics-based 2D matched filter is developed and used to detect and localize the flaws. An iterative solution is developed to sequentially search the resulting 2D image to extract and characterize the flaws.
Distribution of a complex phenolic mixture between water and supercritical carbon dioxide (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDistribution of a complex phenolic mixture between water and supercritical carbon dioxideRobert K. Roop and Aydin AkgermanCite this: J. Chem. Eng. Data 1990, 35, 3, 257–260Publication Date (Print):July 1, 1990Publication History Published online1 May 2002Published inissue 1 July 1990https://pubs.acs.org/doi/10.1021/je00061a010https://doi.org/10.1021/je00061a010research-articleACS PublicationsRequest reuse permissionsArticle Views73Altmetric-Citations11LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Aluminum Oxide Nanoparticle Films Deposited from a Nonthermal Plasma: Synthesis, Characterization, and Crystallization (2020)

Aluminum oxide, both in amorphous and crystalline forms, is a widely used inorganic ceramic material because of its chemical and structural properties. In this work, we synthesized amorphous aluminum oxide nanoparticles using a capacitively coupled nonthermal plasma utilizing trimethylaluminum and oxygen as precursors and studied their crystallization and phase transformation behavior through postsynthetic annealing. The use of two reactor geometries resulted in amorphous aluminum oxide nanoparticles with similar compositions but different sizes. Size tuning of these nanoparticles was achieved by varying the reactor pressure to produce amorphous aluminum oxide nanoparticles ranging from 6 to 22 nm. During postsynthetic annealing, powder samples of amorphous nanoparticles began to crystallize at 800 °C, forming crystalline θ and γ phase alumina. Their phase transformation behavior was found to be size-dependent in that powders of small 6 nm amorphous particles transformed to form phase-pure α-Al<sub>2</sub>O<sub>3</sub> at 1100 °C, while powders of large 11 nm particles remained in the θ and γ phases. This phenomenon is attributed to the fast rate of densification and neck formation in small amorphous aluminum oxide particles.
ETSI RRS - The standardization path to next generation cognitive radio systems (2010)

This paper details the current work status of the ETSI Reconfigurable Radio Systems (RRS) Technical Committee (TC) and gives an outlook on the future evolution. While previous publications have presented an overview of ETSI RRS&amp;#039; main working axes related to i) Cognitive Radio System Aspects, ii) Radio Equipment Architecture (including a Cognitive Pilot Channel (CPC) proposal and a Functional Architecture (FA) for Management and Control of Reconfigurable Radio Systems), iii) Cognitive Management and Control and iv) Public Safety, this document focuses on latest progress related to UHF White Spaces work and the definition of an SDR Handset Architecture. In particular, it is outlined how Cognitive Radio principles can help to adapt existing and/or evolving Radio Standards, such as 3GPP Long Term Evolution, to a possible operation in UHF White Space bands.
Direct Selection of Monoclonal Phosphospecific Antibodies without Prior Phosphoamino Acid Mapping (2009)

In the current post-genomic era, large scale efforts are underway to functionally explore the proteome by assembling large antibody libraries. However, because many proteins are modified post-translationally to regulate their function, collections of modification-specific sensors are also needed. Here we applied a novel approach to select monoclonal phosphospecific antibodies directly from the full-length protein and without up-front phosphoamino acid identification. We chose as antigen GRASP65, a well studied Golgi phosphoprotein. Bacterially produced full-length protein was first incubated with mitotic cytosol, thus allowing modification by naturally occurring kinases, and then used directly for affinity-based antibody selection using a single chain variable fragment phagemid library. In less than 1 week, three distinct and highly functional monoclonal phosphospecific antibodies against two GRASP65 epitopes were obtained and subsequently characterized. The presented approach is carried out fully in vitro, requires no prior knowledge of the phosphoamino acid identity, and is fast and inexpensive. It therefore has great potential to be an attractive alternative to classic animal-based protocols for the selection of post-translation modification sensors and thus to become an invaluable tool in our quest to understand the proteome in all its complexity.
Comparison of 4 methods for quantification of dopamine transporters by SPECT with [123I]IACFT. (2000)

These results show that BP can be estimated consistently using 4 different methods. This finding lends support to the modeling assumptions and provides methods suitable for clinical investigation.
Rapid Colorimetric pH-Responsive Gold Nanocomposite Hydrogels for Sensing Applications (2022)

Surface functionalization of metallic nanoparticles (NPs) with external groups can be engineered to fabricate sensors that are responsive to various stimuli like temperature, pH, and numerous ions. Herein, we report the synthesis of gold nanoparticles (GNPs) functionalized with 3-mercaptopropionic acid (GNPs-MPA) and the doping of these nanoparticles into hydrogel materials using the breathing-in/breathing-out (BI-BO) method. MPA has a carboxyl group that becomes protonated and, thus, ionized at a pH below its pK<sub>a</sub> (4.32); hence, the GNPs-MPA solutions and gels were mostly pH-responsive in the range of 3-5. Optical properties were assessed through ultraviolet-visible (UV-Vis) spectroscopy, namely: transmission and absorption, and the parameters used to quantify the pH changes were the full width at half maximum (FWHM) and position of surface plasmon resonance (SPR). The solutions and gels gradually changed their colors from red to indigo with pH decrementation from 5 to 3, respectively. Furthermore, the solutions' and doped gels' highest FWHM sensitivities towards pH variations were 20 nm and 55 nm, respectively, while the SPR's position sensitivities were 18 nm and 10 nm, respectively. Also, transmission and scanning electron microscopy showed synchronized dispersion and aggregation of NPs with pH change in both solution and gel forms. The gel exhibited excellent repeatability and reversibility properties, and its response time was instantaneous, which makes its deployment as a colorimetric pH-triggered sensor practical. To the best of our knowledge, this is the first study that has incorporated GNPs into hydrogels utilizing the BI-BO method and demonstrated the pH-dependent optical and colorimetric properties of the developed nanocomposites.
High‐throughput screening for novel allosteric modulators of the D1 dopamine receptor (662.4) (2014)

Dopamine is a critically important neurotransmitter in the CNS and peripheral nervous system, with five receptor subtypes mediating its effects. Importantly, D1 receptor (D1R)‐selective agents may prove beneficial in the treatment of many neuropsychiatric disorders. In an effort to identify novel selective allosteric modulators of the D1R we used a high throughput screening approach. 380,000 small molecules in the NIH Molecular Libraries Screening Center Network library were screened using a cell line expressing the D1R coupled to G15 resulting in a robust Ca2+ signal upon receptor stimulation. Hit compounds were triaged through secondary functional and radioligand displacement binding assays to determine subtype selectivity and their allosteric versus orthosteric nature. We initially identified 96 putative positive allosteric modulator (PAM) hits that enhanced the EC20 activity of dopamine in the Ca2+ response ‐ 6 of these were subsequently confirmed during triage and were chosen for further characterization. In addition we found approximately 115 agonist and 125 antagonist hits that failed to completely inhibit radioligand binding and have been classified as potential allosteric agonists and antagonists or negative allosteric modulators (NAMs). These compounds are currently being characterized using additional assays to confirm their selectivity, activity, and classification. Grant Funding Source : NINDS Intramural Program
Transfusion‐associated transmissionof babesiosis in New York State (2000)

Infected donors lived in endemic areas and were asymptomatic with no history of tick bite. Blood collected in January 1997 from one donor was infectious. Those transfusion recipients who were infected were neonatal, elderly, or chronically transfused patients. Babesiosis should be included in the differential diagnosis of febrile illness in immunocompromised recipients of blood transfusion, particularly in the Northeastern United States.
Impact of partial oxidation in dense CO2 on the solid-state properties of (VO)2P2O7 catalyst (2002)

(VO)2P2O7 was used as a catalyst for the partial oxidation of propane to oxygenates (acrylic acid and acetic acid) in dense carbon dioxide (2–10 MPa) at 673 K using air as the oxidant. The catalytic studies showed a decreasing yield of acrylic acid with higher reaction pressure, with the opposite effect found for acetic acid. One of the reasons for such behaviour has recently been found to be a stronger interaction of the catalyst and the olefinic double bond via catalyst Lewis sites. Another reason could be changing vanadium oxidation state in the solid catalyst. Therefore, detailed XPS and 31P NMR spectroscopic studies using the spin echo mapping technique were carried out to reveal more information on the possible pressure dependency of the vanadium oxidation state. The investigations clearly revealed an increasing vanadium oxidation state in the direction of V5+ with increasing reaction pressure. This might be due to a higher oxygen concentration on the catalyst surface. The decrease in acrylic acid yield also seems to be due to the observed increase in the vanadium oxidation state leading to over-oxidation of a greater extent.
Facile preparation of a novel high performance benzoxazine–CNT based nano-hybrid network exhibiting outstanding thermo-mechanical properties (2013)

Untreated multi-walled carbon nanotubes (MWCNTs) have been dispersed in two benzoxazine precursors following an easy procedure. The strong intrinsic interactions of CNTs with a selected precursor give rise to the formation of a reinforced network with outstanding thermo-mechanical properties.
Supplementary Figure S10 from CD142 identifies neoplastic desmoid tumor cells, uncovering interactions between neoplastic and stromal cells that drive proliferation (2023)

&lt;p&gt;Densitometry of selected secreted proteome from mutant and non-mutant conditioned media&lt;/p&gt;
Opposite microglial activation stages upon loss of <scp>PGRN</scp> or <scp>TREM</scp> 2 result in reduced cerebral glucose metabolism (2019)

Microglia adopt numerous fates with homeostatic microglia (HM) and a microglial neurodegenerative phenotype (MGnD) representing two opposite ends. A number of variants in genes selectively expressed in microglia are associated with an increased risk for neurodegenerative diseases such as Alzheimer's disease (AD) and frontotemporal lobar degeneration (FTLD). Among these genes are progranulin (<i>GRN</i>) and the triggering receptor expressed on myeloid cells 2 (<i>TREM2</i>). Both cause neurodegeneration by mechanisms involving loss of function. We have now isolated microglia from <i>Grn</i><sup>-/-</sup> mice and compared their transcriptomes to those of <i>Trem2</i><sup>-/-</sup><i>mice</i> Surprisingly, while loss of <i>Trem2</i> enhances the expression of genes associated with a homeostatic state, microglia derived from <i>Grn</i><sup>-/-</sup> mice showed a reciprocal activation of the MGnD molecular signature and suppression of gene characteristic for HM The opposite mRNA expression profiles are associated with divergent functional phenotypes. Although loss of TREM2 and progranulin resulted in opposite activation states and functional phenotypes of microglia, FDG (fluoro-2-deoxy-d-glucose)-μPET of brain revealed reduced glucose metabolism in both conditions, suggesting that opposite microglial phenotypes result in similar wide spread brain dysfunction.
Comparative oxidative stability of α‐tocopherol blended and diffused UHMWPEs at 3 years of real‐time aging (2010)

Vitamin E (α-tocopherol) is a free-radical stabilizing antioxidant used to maintain oxidative stability in radiation cross-linked ultra-high molecular weight polyethylene (UHMWPE) used in total joint replacements. We investigated the oxidative resistance of vitamin E-stabilized UHMWPE in (i) postirradiation vitamin E-diffused UHMWPE, (ii) vitamin E blended and irradiated UHMWPE, and (iii) unstabilized, irradiated UHMWPE after accelerated aging and real-time aging in an aqueous environment at 40°C for 36 months. Unstabilized samples exhibited substantial oxidation throughout the surface and bulk with both types of aging. While vitamin E-stabilized, radiation cross-linked UHMWPEs were all superior to unstabilized samples, irradiated blends showed surface oxidation and subsurface oxidation potential beginning at ten months in real-time aging. In contrast, postirradiation vitamin E-diffused UHMWPEs showed no detectable oxidation and no increase in oxidation potential despite elution of some vitamin E. We also showed that current thermal accelerated aging methods were unable to differentiate among the oxidative stability of vitamin E-stabilized, radiation cross-linked UHMWPEs prepared by different processes.
Improved layer assignment for packaging multichip modules (2003)

The layer assignment problem plays an important role in packaging multichip modules, since the number of layers is directly related to the cost of the final product. In this paper, the authors propose a new model for the problem and a heuristic layer assignment algorithm based on the new model. The experimental results presented show that the solution provided by the algorithm is close to the lower bound.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>