gebhart/patrepeval huggingface dataset:
Data from Endogenous Retroelement Activation by Epigenetic Therapy Reverses the Warburg Effect and Elicits Mitochondrial-Mediated Cancer Cell Death (2023)

&lt;div&gt;Abstract&lt;p&gt;For millions of years, endogenous retroelements have remained transcriptionally silent within mammalian genomes by epigenetic mechanisms. Modern anticancer therapies targeting the epigenetic machinery awaken retroelement expression, inducing antiviral responses that eliminate tumors through mechanisms not completely understood. Here, we find that massive binding of epigenetically activated retroelements by RIG-I and MDA5 viral sensors promotes ATP hydrolysis and depletes intracellular energy, driving tumor killing independently of immune signaling. Energy depletion boosts compensatory ATP production by switching glycolysis to mitochondrial oxidative phosphorylation, thereby reversing the Warburg effect. However, hyperfunctional succinate dehydrogenase in mitochondrial electron transport chain generates excessive oxidative stress that unleashes RIP1-mediated necroptosis. To maintain ATP generation, hyperactive mitochondrial membrane blocks intrinsic apoptosis by increasing BCL2 dependency. Accordingly, drugs targeting BCL2 family proteins and epigenetic inhibitors yield synergistic responses in multiple cancer types. Thus, epigenetic therapy kills cancer cells by rewiring mitochondrial metabolism upon retroelement activation, which primes mitochondria to apoptosis by BH3-mimetics.&lt;/p&gt;Significance:&lt;p&gt;The state of viral mimicry induced by epigenetic therapies in cancer cells remodels mitochondrial metabolism and drives caspase-independent tumor cell death, which sensitizes to BCL2 inhibitor drugs. This novel mechanism underlies clinical efficacy of hypomethylating agents and venetoclax in acute myeloid leukemia, suggesting similar combination therapies for other incurable cancers.&lt;/p&gt;&lt;p&gt;&lt;i&gt;This article is highlighted in the In This Issue feature, p. 995&lt;/i&gt;&lt;/p&gt;&lt;/div&gt;
ChemInform Abstract: Total Synthesis of Olivomycin A. (1999)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Visualization, Analysis, and Design of COMBO-FISH Probes in the Grid-Based GLOBE 3D Genome Platform (2010)

The genome architecture in cell nuclei plays an important role in modern microscopy for the monitoring of medical diagnosis and therapy since changes of function and dynamics of genes are interlinked with changing geometrical parameters. The planning of corresponding diagnostic experiments and their imaging is a complex and often interactive IT intensive challenge and thus makes high-performance grids a necessity. To detect genetic changes we recently developed a new form of fluorescence in situ hybridization (FISH) - COMBinatorial Oligonucleotide FISH (COMBO-FISH) - which labels small nucleotide sequences clustering at a desired genomic location. To achieve a unique hybridization spot other side clusters have to be excluded. Therefore, we have designed an interactive pipeline using the grid-based GLOBE 3D Genome Viewer and Platform to design and display different labelling variants of candidate probe sets. Thus, we have created a grid-based virtual "paper" tool for easy interactive calculation, analysis, management, and representation for COMBO-FISH probe design with many an advantage: Since all the calculations and analysis run in a grid, one can instantly and with great visual ease locate duplications of gene subsequences to guide the elimination of side clustering sequences during the probe design process, as well as get at least an impression of the 3D architectural embedding of the respective chromosome region, which is of major importance to estimate the hybridization probe dynamics. Beyond, even several people at different locations could work on the same process in a team wise manner. Consequently, we present how a complex interactive process can profit from grid infrastructure technology using our unique GLOBE 3D Genome Platform gateway towards a real interactive curative diagnosis planning and therapy monitoring.
Evaluation of a transgenic mouse model for alpha‐1‐antitrypsin (AAT) related liver disease (1994)

We have attempted to produce a transgenic mouse model of the neonatal liver disease associated with the human PIZ allele. Analysis of a number of transgenic mouse lines carrying either a normal human PIM gene construct or the mutant Z is reported. Using isoelectric focusing analysis of plasma from transgenic mice, we have shown that the human AAT proteins produced in mice are processed in a similar way to their counterparts in humans. By comparing the level of M and Z mRNA in liver with the levels of M and Z proteins in plasma we have inferred that, as in humans, the mutant protein tends to accumulate within the hepatocyte. Accumulation of Z protein has also been demonstrated by immunocytochemistry. Two of the M transgenic lines produce such high levels of the human protein that it, like the Z protein, accumulates as globules. Histological features of livers from 116 mice of different ages and genotypes were examined: 37 non-transgenic, 62 Z transgenic (23 low expressing and 39 high expressing) and 17 M transgenic mice, all high expressing. Cirrhosis or fibrosis was not seen in any animal and we were unable to find any evidence for neonatal liver disease. Some necrosis was seen in all genotypes and this increased significantly with age with one Z line showing significantly more frequent necrosis than any other group. This line, the highest expressing Z line, was back crossed onto 7 different genetic backgrounds but no major differences between the back crosses with respect to liver disease were observed. The mouse model we have developed is compared with other transgenic Z mouse models; none of these is representative of human neonatal liver disease. Our view is that the transgenic animals generated in these experiments may be most useful for investigating the liver manifestations that almost invariably occur in ZZ adults. Alteration of additional factors other than accumulation of Z protein, for example inactivation of the endogenous mouse genes or some environmental challenge, might produce a mouse model with more relevance to neonatal liver disease.
Enhanced transdermal efficiency of curcumin-loaded peptide-modified liposomes for highly effective antipsoriatic therapy (2021)

Psoriasis is one of the most influential and fastest-growing inflammatory diseases of the skin. Curcumin (CRC) is an effective antipsoriatic drug that is often carried by nanoparticles or liposomes mainly administered via the skin. However, the therapeutic effectiveness and bioavailability of this drug are restricted due to the functions of the skin barrier to liposomes. Herein, we proposed a peptide-modified curcumin-loaded liposome (CRC-TD-Lip) to expedite the transdermal delivery of curcumin and enhance the inhibition of psoriasis. CRC-TD-Lip was prepared and dispersed uniformly with high stability and high curcumin encapsulation efficiency. We confirmed the improved intracellular uptake of CRC-TD-Lip, the increased inhibitory effect of CRC-TD-Lip on HaCaT cells, and the heightened transdermal ability of CRC-TD-Lip. Then, the enhanced antipsoriatic ability of CRC-TD-Lip was evaluated in vivo using an imiquimod-induced psoriasis mouse model. The results indicated that the developed CRC-TD-Lip can effectively improve the delivery of curcumin across the skin and enhance the antipsoriasis efficiency. This work can provide a strategy for enhancing the transdermal delivery efficiency of drugs for various skin diseases.
ChemInform Abstract: Catalytic Asymmetric Cyclopropanation Using Copper Complex of Optically Active Bipyridine as a Catalyst. (1993)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
New partial pressure gauge for helium and deuterium using the ion backscattering principle (1987)

A new partial pressure gauge is proposed that is capable of measuring partial pressures of He and D2 gases in the mixture. The gauge consists of a conventional ion scattering spectrometer with a Wien filter. The influence of the residual gases CH4 and H2O on the pressure measurements of D2 and He in this gauge is discussed.
Blue-Green Emitting SrS:Ce, Cl Thin-Film Electroluminescent Devices Prepared by Reactive Evaporation Using H<sub>2</sub>S Gas (1991)

SrS:Ce, Cl phosphor films were deposited by evaporating Sr metal and CeCl 3 in H 2 S gas. The crystallinity, luminance, and emission spectra of the films were investigated in relation to the deposition conditions. X-ray diffraction intensity from the (200) plane of the SrS films was found to increase with the substrate temperature when the H 2 S pressure was kept constant, and had the maximum value at the H 2 S pressure of 1×10 -2 Pa when the substrate temperature was kept constant. The variation of luminance in relation to the H 2 S pressure and the substrate temperature was similar to that of the (200) diffraction intensity. The luminance of 600 cd/m 2 and the maximum emission efficiency as high as 1 lm/W were obtained at 1 kHz 30 µs wide ac pulse excitation for the films deposited at 600°C and H 2 S pressure of 1×10 -2 Pa, although the emission color was not blue but whitish green.
Reaction Technology in Asymmetric Synthesis (2007)

This chapter contains sections titled: Reaction Engineering in Asymmetric Synthesis Introduction Membrane Reactors with Chemical Catalysts Membrane Reactors with Biological Catalysts Membrane Reactors with Whole Cells Membrane Reactors with Isolated Enzymes Two-Phase Systems Conclusions Biocatalyzed Asymmetric Syntheses Using Gel-Stabilized Aqueous–Organic Two-Phase Systems Gel-Stabilized Two-Phase Systems Benzoin Condensation with Entrapped Benzaldehyde Lyase Reduction of Ketones with Entrapped Alcohol Dehydrogenase Conclusion
Simulating the dynamic strength of molecular interactions (2001)

It has been demonstrated that the force at which an interaction will break depends on the rate at which force is applied. Here, we present a strategy for the modeling of the forced dissociation of a ligand–receptor interaction, using the streptavidin–biotin complex as an example, over a range of loading rates that are outside those attainable by current all-atom simulation techniques. The method adopted is a combination of traditional reaction coordinate mapping and Brownian dynamics. Our simulations predict a dynamic force spectrum for the streptavidin–biotin interaction of similar form to recent experimental results. In this study we confirm the logarithmic dependence of a rupture force on the loading rate, highlight the barriers that are probed at the loading rates attainable by the atomic force microscope, and discuss how these barriers transform under loading. Furthermore, it is confirmed that additional information obtained from the distribution of rupture forces can be used to complement dynamic force spectroscopy data and should be used in experimental studies to verify the results obtained.
Torque performance of axial flux permanent magnet fractional open slot machine with unequal teeth (2011)

Please help us populate SUNScholar with the post print version of this article. It can be e-mailed to: scholar@sun.ac.za
A Comparative Study on Strategies for Trust Recovery and Acceptance of Nuclear Power in Nuclear Power Plant Areas (2017)

본 연구는 한국의 4대 원전주변 지역 주민들의 원자력 수용성과 신뢰회복 전략을 모색하기 위한 이론적 모형을 구축하고, 이를 개인수준의 미시데이터를 통해서 실증 분석함으로써, 각 원전주변지역별로 독특한 특성을 규명하고, 향후 차별적인 신뢰회복 전략에 대한 함의를 도출하는데 목적이 있다. 즉, 영광, 울진, 고리, 월성의 국내의 4대 원전주변 지역의 주민들을 대상으로 사회인구적 특성, 위험지각 요인(지각된 위험와 편익, 낙인, 신뢰, 지식), 위험소통 요인(정보 질, 부정적 정보 량), 원자력 관련 사회적 사건(후쿠시마 사고, 원전비리), 지역특성(경제, 정치, 정체성) 등을 통합한 모형을 중심으로 원자력 수용성과 신뢰회복 전략(선호)에 미치는 영향을 규명하였다. 이를 통해서 각 지역별 독특한 차이를 다각적으로 살펴보고, 최근의 원자력 불신구조의 향후 방향성을 모색함에 있어서 지역별 특성을 고려한 신뢰회복 전략에 대한 시사점을 논의하였다.This study aims to search for strategies to improve local residents’ acceptance of nuclear power and trust recovery among people living near nuclear power station. For this objective, we built a theoretical model and empirically analyzed the micro-data at the individual level to highlight the particular attribute of each nuclear site, suggesting the implication of strategies for trust recovery. We examined the impact of (1) socio-demographic variables (gender, education, economic status, age, social class, ideology, and religion), (2) perceived risk (perceived risk, perceived benefit, trust, stigma, knowledge), (3) risk communication (quality of information, quantity of negative information), (4) social event (Fukushima accident, and corruption of KHNP), and (5) localities (economic, political characteristics and identity) on acceptance of nuclear power and strategies for trust recovery based on responses from local residents in four nuclear power stations in Korea. We discussed the implications of strategies for trust recovery considering the characteristics of localities.
Factitious hypoglycemia in insulin-treated diabetic patients (2023)

Factitious hypoglycemia is a factitious disorder according to the Diagnostic and Statistical Manual of Mental Disorders (DSM-5), referring to intentionally covertly induced hypoglycemia, with potentially severe consequences. Knowledge of factitious hypoglycemia relies on case reports, and evidence-based information and guidelines are lacking. Diagnosing factitious hypoglycemia in insulin-treated diabetic persons is therefore challenging and often requires a long and costly process. Moreover, the typical metrics proposed to differentiate insulin-induced factitious hypoglycemia from insulinoma (i.e., high insulin and low C-peptide versus high insulin and high C-peptide, respectively) are not always applicable, depending on whether the insulin quantification method can detect the insulin analog. When factitious hypoglycemia is suspected, an emerging trend from recent publications advocates a combination of two insulin quantification methods with different cross-reactivity for insulin analogs, early on in the diagnostic process.
Correction: The Use of Small Molecules to Investigate Molecular Mechanisms and Therapeutic Targets for Treatment of Botulinum Neurotoxin A Intoxication (2006)

ADVERTISEMENT RETURN TO ISSUEPREVCorrectionCorrection: The Use of Small Molecules to Investigate Molecular Mechanisms and Therapeutic Targets for Treatment of Botulinum Neurotoxin A IntoxicationTobin J. Dickerson and Kim D. JandaCite this: ACS Chem. Biol. 2006, 1, 7, 470Publication Date (Web):August 28, 2006Publication History Received2 August 2006Accepted2 August 2006Published online28 August 2006Published inissue 1 August 2006https://pubs.acs.org/doi/10.1021/cb600333chttps://doi.org/10.1021/cb600333ccorrectionACS PublicationsCopyright © 2006 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views387Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (66 KB) Get e-AlertscloseSUBJECTS:Chemical structure,Hydrocarbons,Mechanisms of action,Small molecules,Toxicology Get e-Alerts
Genetic and Transcriptomic Analysis Reveal the Molecular Basis of Photoperiod-Regulated Flowering in Xishuangbanna Cucumber (Cucumis sativus L. var. xishuangbannesis Qi et Yuan) (2021)

Xishuangbanna (XIS) cucumber (<i>Cucumis sativus</i> L. var. <i>xishuangbannesis</i> Qi et Yuan), is a botanical variety of cucumber cultivars native to southwest China that possesses excellent agronomic traits for cucumber improvement. However, breeding utilization of XIS cucumber is limited due to the current poor understanding of its photoperiod-sensitive flowering characteristics. In this study, genetic and transcriptomic analysis were conducted to reveal the molecular basis of photoperiod-regulated flowering in XIS cucumber. A major-effect QTL locus <i>DFF1.1</i> was identified that controls the days to first flowering (DFF) of XIS cucumbers with a span of 1.38 Mb. Whole-genome re-sequencing data of 9 cucumber varieties with different flowering characteristics in response to photoperiod suggested that <i>CsaNFYA1</i> was the candidate gene of <i>DFF1.1</i>, which harbored a single non-synonymous mutation in its fifth exon. Transcriptomic analysis revealed the positive roles of auxin and ethylene in accelerating flowering under short-day (SD) light-dark cycles when compared with equal-day/night treatment. Carbohydrate storage and high expression levels of related genes were important reasons explaining early flowering of XIS cucumber under SD conditions. By combining with the RNA-Seq data, the co-expression network suggested that <i>CsaNFYA1</i> integrated multiple types of genes to regulate the flowering of XIS cucumber. Our findings explain the internal regulatory mechanisms of a photoperiodic flowering pathway. These findings may guide the use of photoperiod shifts to promote flowering of photoperiod-sensitive crops.
A 1.25/10.3-Gbit/s AC-coupled dual-rate burst-mode receiver without reset signals (2008)

We propose and demonstrate a 1.25/10.3-Gbit/s dual-rate burst-mode receiver with two gate circuits to separate the signals according to the bit rate of the input signals. This receiver is cost effective because it employs devices with an ac-coupled interface and no reset signals.
Central role for phosphoinositide-3-kinase gamma/delta dependent signalling in eosinophilic pulmonary inflammation driven by innate lymphoid cells (2017)

Severe eosinophilic asthma remains a clinical burden, potentially due to the multiple mechanisms by which type 2 inflammation can arise. Here we show how epithelial alarmin stimulation of innate lymphoid type 2 cells (ILC2s) can promote Th2 cell activity and subsequent eosinophil influx to the lung via mechanisms dependent upon PI3Kγ and δ isoform activity. A PI3Kgamma and delta (PI3Kγδ) mRNA signature identified a Ubiopred severe asthmatic patient segment with high sputum eosinophil content and a Th2/ILC2/alarmin endotype. Evaluation of potent PI3K inhibitors with γ, δ or dual isoform selectivity supported a role in this endotype as follows: PI3Kγ mediated eosinophil degranulation in an in vitro asthmatic subject blood assay. PI3Kδ-dependent IL-33 signalling/ILC2 activity was observed in an in vitro blood assay. In vivo activity of an inhaled PI3Kγδ inhibitor was demonstrated within a murine mechanistic model of IL-33/ILC2-dependent eosinophil influx, and further confirmed using PI3Kγ knockout and PI3Kδ kinase dead knock-in mice. Finally, we demonstrated therapeutic efficacy of PI3Kγδ pathway inhibition on Th2 mediator release and subsequent eosinophil influx in a rodent in vivo model of pulmonary inflammation. These data indicate a central role for both PI3Kγ and δ isoforms in eosinophilic pulmonary inflammation driven by epithelial damage and innate lymphoid cells, and thereby highlight the therapeutic potential of an inhaled dual PI3Kγδ dual inhibitor in severe eosinophilic asthma.
Progressive Increases in Complexity of T-Wave Oscillations Herald Ischemia-Induced Ventricular Fibrillation (2002)

T-wave alternans (TWA), an ABAB oscillation, has been postulated as the initial pattern in a stepwise progression to higher-order oscillations, culminating in sudden arrhythmic death. The present study is the first to provide experimental evidence to support this intriguing concept. Epicardial and endocardial ECGs from 12 dogs were monitored during 8-minute left anterior descending coronary artery occlusion with right atrial pacing at 150 bpm. TWA magnitude was measured by modified moving average beat analysis, and the complexity of T-wave oscillations was assessed by complex demodulation. In 6 animals with subsequent ventricular fibrillation (VF), TWA achieved a threshold of 5.00+/-1.30 mV in epicardial ischemic-zone electrograms, which then exhibited a stepwise increase in T-wave oscillation complexity to quadrupling (ABCDABCD, 3 cases) or tripling (ABCABC, 2 cases) and to more complex forms (5 cases) preceding VF (6 cases). In dogs without VF, peak TWA levels did not increase from baseline, measuring a maximum of 0.35+/-0.10 mV (P=NS), or only 7% the value of those with VF, and T-wave multupling was not observed (0 of 6 versus 5 of 6, P<0.005). Discordant TWA episodes, with T waves alternating out of phase, were associated with increased T-wave complexity and fibrillation in 4 of 6 dogs with VF but in none of the 6 dogs without VF (P<0.025). TWA appears to be the first step in an orderly progression of T-wave complexity, episodes of discordant TWA, and VF. This demonstrated increase in T-wave complexity points to a fundamental mechanistic link underlying the ability of TWA to predict lethal arrhythmias.
IS IT REASONABLE TO PROPOSE AN ENDOSCOPIC MUCOSAL RESECTION FOR BARRET'S OESOPHAGUS WITH HIGH GRADE DYSPLASIA ON THE BIOPSIES? (2018)

Endoscopic mucosal resection (EMR) is currently the treatment for high grade dysplasia (HGD) in Barret's esophagus (BE), based on biopsies. The aim was to evaluate the discordance between biopsy sample (BS) and EMR specimen in case of HGD, and cytologists’ inter and intra-observatory variability.