gebhart/patrepeval huggingface dataset:
In vitro resistance profile of hepatitis C virus NS5A inhibitor velpatasvir in genotypes 1 to 6 (2019)

Velpatasvir is a pan-genotypic hepatitis C virus (HCV) NS5A inhibitor, which is used with sofosbuvir for treatment of infection with HCV genotypes 1-6. In vitro resistance studies were performed to characterize NS5A changes that might confer reduced velpatasvir susceptibility in vivo. Resistance selection studies using HCV replicon cells for subtypes 1a, 1b, 2a, 2b, 3a, 4a, 5a and 6a identified NS5A resistance-associated substitutions (RASs) at nine positions, most often 28M/S/T, 31F/I/M/P/V and 93D/H/N/S. In subtype 1a, RASs were selected at positions 31 and/or 93, while in subtype 1b, replicons with two or more RASs at positions 31, 54 or 93 were selected. Y93H was selected in subtypes 1a, 1b, 2a, 3a and 4a. In subtype 5a or 6a, L31P or P32L/Q was selected, respectively. Velpatasvir susceptibility of 358 replicons from genotypes 1 to 6 containing one or more NS5A RASs was also evaluated. The majority (63%) of subtypes 1a and 1b single RAS-containing replicons retained susceptibility to velpatasvir (<2.5-fold change in EC<sub>50</sub> ). High levels of resistance to velpatasvir were observed for six single mutants in subtype 1a, including M28G, A92K, Y93H/N/R/W and for one mutant, A92K, in subtype 1b. Most single mutants in subtypes 2a, 2b, 3a, 4a and 5a displayed low levels of reduced velpatasvir susceptibility. High-level resistance was observed for C92T and Y93H/N in subtype 2b, Y93H/S in 3a, and L31V and P32A/L/Q/R in 6a, and several double mutants in these subtypes. Overall, velpatasvir maintained activity against most common RASs that are known to confer resistance to first-generation NS5A inhibitors.
Time course of DNA adduct formation in peripheral blood granulocytes and lymphocytes after drinking alcohol (2012)

Alcohol consumption is an established risk factor for cancers of the head and neck, colorectum, liver and female breast. Acetaldehyde, the primary metabolite of ethanol, is suspected to play a major role in alcohol-related carcinogenesis. Acetaldehyde binds to DNA resulting in formation of adducts. DNA adducts are involved in mutagenesis and carcinogenesis. N (2)-Ethylidenedeoxyguanosine (N (2)-ethylidene-dGuo) is the major adduct formed in this reaction. Studies have shown an association between alcohol drinking and levels of this DNA adduct, suggesting its potential use as a biomarker for studying alcohol-related carcinogenesis. However, there are no reports on the kinetics of formation and repair of N (2)-ethylidene-dGuo after alcohol consumption. Therefore, we investigated levels of N (2)-ethylidene-dGuo in DNA from human peripheral blood cells at several time points after consumption of increasing doses of alcohol. Ten healthy non-smokers were recruited and asked to abstain from alcohol consumption except for the study doses. The subjects were given measured doses of alcohol once a week for 3 weeks, targeting increasing blood alcohol levels. Blood was collected at several time points before and after each dose, DNA was isolated from granulocytes and lymphocytes and N (2)-ethylidene-dGuo was quantified as its NaBH(3)CN reduction product N ( 2 )-ethyldeoxyguanosine by liquid chromatography-electrospray ionisation-tandem mass spectrometry. Significant increases in N (2)-ethylidene-dGuo were observed after all doses and in both cell types. However, there was substantial intraindividual variability, indicating that there are other important sources of this adduct in peripheral blood DNA. Further studies are needed to better understand the origins of N (2)-ethylidene-dGuo in blood cells, the exposures it reflects, and thus its potential use as a marker of alcohol's genotoxic effects.
Quantum shot noise reduction for electron field emission from different shapes (2010)

Shot noise is the electronic noise that occurs due to the discreteness of electronic charge and has a uniform frequency spectrum. The study of shot noise suppression plays an important part in studying wave-particle duality in mesoscopic devices. Quantum partitioning at high fields leads to significant electron correlation during the tunneling process. Previous models developed to study quantum shot noise suppression have been one-dimensional (1-D) with a constant field enhancement factor. In order to take into account the geometric variation of the field enhancement factor across the surface of the emitter, a two-dimensional (2-D) model is presented in this paper.
White Space Prediction for Low-Power Wireless Networks: A Data-Driven Approach (2018)

In the 2.4 GHz unlicensed spectrum, the coexistence of WiFi, Bluetooth and IEEE 802.15.4 devices generates increased channel contention. Notably, low-power wireless networks experience packet loss and delays due to interference. To improve the performance of low-power wireless networks under interference, we propose a data driven proactive approach based on interference modeling for white space prediction. We leverage statistical analysis of real-world traces from two indoor environments characterized by varying channel conditions to identify interference patterns. We characterize interference in terms of Inter-Arrival Time (IAT) and number of interfering signals and use a Gaussian Mixture Model (GMM) to accurately estimate the interference distribution as observed by the low-power wireless nodes. Then, we use a Hidden Markov Model (HMM) for white space prediction. Our validation w.r.t. real-world traces from two environments show that our GMM model can estimate interference with an accuracy higher than 94:7%. Moreover, the white space prediction evaluation shows an average accuracy of 97:7% and 89:5% across the two environments.
Precise Survey of Dokaebi Road Using CDGPS (1999)

Using GPS carrier phase whose cycle ambiguities are resolved, it is possible to perform precise survey requiring centimeter-level positioning accuracy. Because of an optical illusion, we cannot recognize the exact slope of Dokaebi Road. In this paper, we performed kinematic survey experiments in order to calculate the exact slope of Dokaebi Road with high positioning accuracy of CDGPS. By post-processing experimental data using CDGPS, it was possible to generate the exact vertical trajectory of Dokaebi Road with centimeter-level accuracy.
Observation of a Short-Wavelength Laser Pumped by Auger Decay (1986)

We report the observation of gain in a new type of short-wavelength laser system at 108.9 nm. Soft x rays from a laser-produced plasma photoeject an inner-shell electron from xenon, and a population inversion is created by subsequent Auger decay to particular excited states. A model for this system is described, spectroscopic data are presented, and gain limitations due to parasitic amplified spontaneous emission are discussed. A maximum gain of 0.8 ${\mathrm{cm}}^{\ensuremath{-}1}$ over a length of 9 cm is reported.
Unreplicated DNA remaining from unperturbed S phases passes through mitosis for resolution in daughter cells (2016)

To prevent rereplication of genomic segments, the eukaryotic cell cycle is divided into two nonoverlapping phases. During late mitosis and G1 replication origins are "licensed" by loading MCM2-7 double hexamers and during S phase licensed replication origins activate to initiate bidirectional replication forks. Replication forks can stall irreversibly, and if two converging forks stall with no intervening licensed origin-a "double fork stall" (DFS)-replication cannot be completed by conventional means. We previously showed how the distribution of replication origins in yeasts promotes complete genome replication even in the presence of irreversible fork stalling. This analysis predicts that DFSs are rare in yeasts but highly likely in large mammalian genomes. Here we show that complementary strand synthesis in early mitosis, ultrafine anaphase bridges, and G1-specific p53-binding protein 1 (53BP1) nuclear bodies provide a mechanism for resolving unreplicated DNA at DFSs in human cells. When origin number was experimentally altered, the number of these structures closely agreed with theoretical predictions of DFSs. The 53BP1 is preferentially bound to larger replicons, where the probability of DFSs is higher. Loss of 53BP1 caused hypersensitivity to licensing inhibition when replication origins were removed. These results provide a striking convergence of experimental and theoretical evidence that unreplicated DNA can pass through mitosis for resolution in the following cell cycle.
Packed column supercritical fluid chromatography using deactivated stationary phases (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPacked column supercritical fluid chromatography using deactivated stationary phasesM. Ashraf-Khorassani, L. T. Taylor, and R. A. HenryCite this: Anal. Chem. 1988, 60, 15, 1529–1533Publication Date (Print):August 1, 1988Publication History Published online1 May 2002Published inissue 1 August 1988https://pubs.acs.org/doi/10.1021/ac00166a011https://doi.org/10.1021/ac00166a011research-articleACS PublicationsRequest reuse permissionsArticle Views83Altmetric-Citations37LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
SURFACE STUDIES OF NICKEL (1981)

The coordination chemistry of a range of molecules of catalytic importance adsorbed on nickel surfaces has been investigated.These studies have been performed as a function of surface crystallography and .composition.Five crystallographic planes were utilized; the three low Miller index Ni (111), Ni(llO) and Ni(lOO), the stepped Ni 9(lll)x(lll) and the stepped-kinked Ni 7(lll)x(310) with carbon, sulfur and oxygen introduced as surface contaminants .. Detailed studies of the aromatic hydrocarbons, benzene and toluene, were performed.Thermal desorption spectroscopy and chemical displacement reactions were used in conjunction with isotope exchange reactions to delineate key factors that control C-H bond activation for these molecules adsorbed on nickel surfaces.Reactive studies of the cyclic hydrocarbons, cyclohexane, cyclohexene, 1 ,3-.and1,4-cyclohexadiene, to form benzene were carried out using the same approach as for the arenes. .Insight into the mechanistic deta i 1 s of the dehydrogenation reaction was gained on the basis of these experiments.Additionally, the surface catalyzed isomerization of CH 3 NC to form ~H 3 GN was observed on carbon or carbon and nitrogen contaminated nickel surfaces.The isomerization did not occur on clean nickel surfaces, thus -iii-demonstrating a dramatic dependence of the chemistry on surface composition.Isotopic exchange reactions, chemical displacement, thermal desorption spectroscopy, complimentary catalytic reactions and high resolution electron energy loss spectroscopy were the techniques used to ., .obtain structural, stereochemical and mechanistic information.Preliminary studies of chemisorbed pyridine, ethylene and acetylene are also described.-iv- ,.I. II.III.... IV .' 1f' .v .. VI.VI I. VIII.IX.' with assignments based on gas phase infrared data.On the clean Ni(lll) surface, the most intense feature in the spectrum wai a 1 oss in the frequency range of 1700-1760 em. 1 , assigned as the CN ' .
BioVision Alexandria 2012, Bibliotheca Alexandrina Science's Quest for a Betther Future. Some Reflections (by video). (2012)

International audience
Recommendations for the use of etanercept in psoriasis: a European dermatology expert group consensus (2006)

The consensus presented herein provides valuable clarification of use of etanercept according to the label, which may have wider implications relating to the use of all biological therapies in psoriasis.
Establishment of a Workflow to Assess Pore Collapse Mechanics During a Series of Loading and Unloading Using Micro-CT Scan and Rock Mechanics Testing (2023)

Abstract Characterization of the reservoir rock elastic and plastic behavior is essential to set a series of guidelines for reservoir management, especially during gas storage cyclicity. The reservoir rock is subjected to loading during injection and unloading during depletion. These procedures lead to increases and decreases in the magnitude of the effective stresses in the poroelastic media. The guidelines for the reservoir development should be set to operate the field within the elastic limit of the formation to ensure the undamaged permeability of continuous fluid flow in and out of the reservoir. Based on these factors, there is a need to accurately measure the dynamic permeability and porosity by characterizing the pore space and pore throat collapse for the current and future injection and depletion operations. This invention will achieve this measurement through a series of alternating measures between a three-dimensional (3D) X-ray computed tomography (CT) imaging and triaxial testing. These accurate and actual measurements mainly come from core plugs testing. The process starts (T1) by scanning the core plugs with high-resolution imaging technology to map pore space and pore throat. The samples will be subjected to the first step of loading and unloading during Thick Wall Cylinder (TWC) test using different axial and confining pressure. The samples will be imaged and then mapped in the pore space and pore throat at a time (T2), and these processes will continue for ten cycles of loading and unloading. The results will be interpreted and extrapolated to match the number of injection and depletion scenarios. The fluid flow will be numerically simulated at the pore scale using direct flow modeling within the pore space with the Navier-Stokes-Brinkman equation to evaluate changes in permeability and porosity. Using these novel integrated processes presented by this invention, injection and depletion guidelines will be set for future operations; this methodology also can be used for oil recovery to ensure safe operations within an elastic limit to avoid permeability damage that will lead to safe, effective, and economical operational scenarios.
A New Class of HIV-1 Integrase Inhibitors:  The 3,3,3‘,3‘-Tetramethyl-1,1‘-spirobi(indan)-5,5‘,6,6‘-tetrol Family (2000)

Integration is a required step in HIV replication, but as yet no inhibitors of the integration step have been developed for clinical use. Many inhibitors have been identified that are active against purified viral-encoded integrase protein; of these, many contain a catechol moiety. Though this substructure contributes potency in inhibitors, it is associated with toxicity and so the utility of catechol-containing inhibitors has been questioned. We have synthesized and tested a systematic series of derivatives of a catechol-containing inhibitor (1) with the goal of identifying catechol isosteres that support inhibition. We find that different patterns of substitution on the aromatic ring suffice for inhibition when Mn(2+) is used as a cofactor. Importantly, the efficiency is different when Mg(2+), the more likely in vivo cofactor, is used. These data emphasize the importance of assays with Mg(2+) and offer new catechol isosteres for use in integrase inhibitors.
REAlease™ Immunomagnetic Separation Technology with reversible labeling for positive selection of leukocytes (2018)

Abstract Immunomagnetic enrichment of leukocytes is an important technique in both research and clinical applications. Accordingly, there is a continuous need for improved, reliable cell separation methods. Current enrichment strategies using magnetic labeling of the target cells allow for highly efficient isolation, but in some instances removal of residual cell surface labeling after isolation is of high interest. To this end, we have combined the benefits of positive selection by MACS® Technology, the proven state-of-the-art method for the isolation of functional, viable cells, with a novel technology enabling the removal of both superparamagnetic beads and antibody fragments. REAlease™ Technology provides an easy and fast solution for the highly specific isolation of unlabeled leukocytes directly from PBMCs. We present our latest results on cell separation with REAlease Technology based on key human immune cell markers, i.e., CD3, CD4, CD8, CD19, and CD56. The benefits of these releasable labels are demonstrated for the isolation of important subsets such as CD3−CD56+ NK cells, and CD4+ and CD8+ cell populations from a single sample. Using various assays, we show that functionality and viability of the target cells are preserved without cell activation.
Poly(ethylene-Co-vinyl Alcohol)/Titanium Dioxide Nanocomposite: Preparation and Characterization of Properties for Potential Use in Bone Tissue Engineering (2022)

A series of poly(ethylene-co-vinyl alcohol)/titanium dioxide (PEVAL/TiO2) nanocomposites containing 1, 2, 3, 4 and 5 wt% TiO2 were prepared by the solvent casting method. These prepared hybrid materials were characterized by Fourier-transform infrared (FT-IR), X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and scanning electron microscopy (SEM). The pores and their interconnections inside these nanocomposites were created using naphthalene microparticles used as a porogen after having been extracted by sublimation under a high vacuum at temperatures slightly below the glass transition temperature. A cellular activity test of these hybrid materials was performed on human gingival fibroblast cells (HGFs) in accordance with ISO 10993-5 and ISO 10993-12 standards. The bioviability (cell viability) of HGFs was evaluated after 1, 4 and 7 days using Alamar Blue®. The results were increased cell activity throughout the different culture times and a significant increase in cell activity in all samples from Day 1 to Day 7, and all systems tested showed significantly higher cell viability than the control group on Day 7 (p < 0.002). The adhesion of HGFs to the scaffolds studied by SEM showed that HGFs were successfully cultured on all types of scaffolds.
My Precious Spine &amp; I: A Self-Care Manual for You and Your Spine<i>Eugene J. van Wyk</i> (2006)

"My Precious Spine & I: A Self-Care Manual for You and Your SpineEugene J. van Wyk." Physiotherapy Canada, 58(3), p. 245
Chemorad Nanoparticles: A Novel Multifunctional Nanoparticle Platform for Targeted Delivery of Concurrent Chemoradiation (2010)

We believe that the ChemoRad NP represents a new class of therapeutics that holds great potential to improve cancer treatment.
Cardiac Hypertrophy Is Inhibited by a Local Pool of cAMP Regulated by Phosphodiesterase 2 (2015)

Different cAMP pools have opposing effects on cardiac myocyte cell size. PDE2 emerges as a novel key regulator of cardiac hypertrophy in vitro and in vivo, and its inhibition may have therapeutic applications.
Table S1 from Galectin-3, a Druggable Vulnerability for KRAS-Addicted Cancers (2023)

&lt;p&gt;Characteristics of cell lines&lt;/p&gt;
Abstract 13968: AMPK Regulates Cardiac Looping by Phosphorylating CLIP-170 that Determines the Directional Cell Migration and Polarity (2010)

Introduction: AMP-activated protein kinase (AMPK) is a key regulator of energy metabolism not only in metabolic organs but also in the heart as evidenced by AMPK gene-modified mice. However, the downstream targets of AMPK that regulates physiological function in the heart have not been fully identified. Methods and Results: To identify a novel AMPK substrate, we performed unique two-step column chromatography. We identified a novel AMPK substrate; cytoplasmic linker protein of 170 KDa (CLIP-170). CLIP-170 is one of the microtubule plus-end proteins regulating microtubule dynamics. AMPK phosphorylates Ser 311 of CLIP-170 both in vitro and in vivo . Inhibition of AMPK or expression of a non-phosphorylatable CLIP-170 mutant in cultured cells resulted in prolonged and increased accumulation of CLIP-170 at microtubule plus ends and slower tubulin polymerization/depolymerization. The failure of microtubule dynamics caused by the defect of CLIP-170 phosphorylation by AMPK led to stabilization of microtubules and perturbed cell migration. All of these phenotypes were rescued by expression of a phosphomimetic CLIP-170 mutant, supporting the importance of CLIP-170 phosphorylation by AMPK. Consistently, in vivo , zebrafish specifically expressing non-phosphorylatable CLIP-170 in heart showed abnormal heart looping without the commitment of cardiac precursor cell to cardiomyocytes, indicating the essential role of AMPK for cardiomyocyte migration. Conclusions: AMPK controls basic cellular functions by regulating microtubule dynamics through CLIP-170 phosphorylation. This newly identified signalling pathway determines the cellular and tissue polarity in the heart.
Polyurethane Powder Coatings: A Review of Composition and Characterization (2020)

Powder coatings have gained a lot of attention since the 1960s and the 1970s (implementation of polyurethanes into the market) because of the environmental and ecological concerns. Over the decades, there have been several publications relating the manufacturing and development of powder coatings as an alternative for liquid/solvent or water-based organic coatings. In general, powder coatings can be divided into two main groups based on the properties of the resin being used, thermoplastic and thermoset powder coating systems. Among thermoset powder coatings, polyurethane powder coatings have outstanding properties such as weather stability and are the best choice for exterior applications comparing overall properties. This paper reviews the most recent advances in the field of powder coating by focusing on polyurethane powder coating as a group of thermoset powder coatings. It also considers the industrial problems engaged in this field, such as emission of the blocking agents, the low reactivity of uretdione systems, and high curing temperature and their solutions. Finally, it also suggests the future potential investigation of polyurethane powder coating compositions and improvements.
Relation of intraventricular conduction delay to risk of new-onset heart failure and structural heart disease in the general population (2020)

LBBB and non-specific IVCD were associated with more than three-fold risk of new-onset HF. Furthermore, LBBB was associated with novel SHD. Their presence should alert clinician even in subjects free from any known heart disease.
Crystal and electronic structures of lithium fluorosulphate based materials for lithium-ion batteries (2010)

By means of ab initio calculations, we study the electronic structure of ${\text{LiFeSO}}_{4}\text{F}$, which has been shown recently [N. Recham et al., Nature Mater. 9, 68 (2010)] to be an excellent material as a cathode for lithium-ion batteries. We have obtained the crystal structure of ${\text{FeSO}}_{4}\text{F}$, which was not completely determined experimentally, and compare the one of ${\text{LiFeSO}}_{4}\text{F}$ to available experimental values, resolving the issue about the position of the Li ion. Our calculated intercalation voltage (3.69 V) is found to be in excellent agreement with experiments. Finally, we have studied ${\text{LiCoSO}}_{4}\text{F}$ and ${\text{LiNiSO}}_{4}\text{F}$, which are found to share a similar crystal structure with ${\text{LiFeSO}}_{4}\text{F}$.