gebhart/patrepeval huggingface dataset:
Pharmacological Retention of Oral Mucosa Progenitor/Stem Cells (2009)

Oral mucosa progenitor/stem cells reside as a small-sized cell population that eventually differentiates concurrently with an increase in cell size. Activation of the mammalian target of rapamycin (mTOR) leads to an increase in cell size. We hypothesized that rapamycin, a specific inhibitor of mTOR, will maintain primary human oral keratinocytes as a small-sized, undifferentiated cell population capable of retaining their proliferative capacity. Primary, rapamycin-treated (2 nM, 20 nM) oral keratinocytes showed a diminished cell size that correlated with a higher clonogenicity, a longer-term proliferative potential, and a slower cycling cell population concurrent with decreased expression of a differentiation marker when compared with untreated cells. Only the 2-nM rapamycin-treated oral keratinocytes maintained their ability to regenerate oral mucosa in vitro after 15 weeks of culture. Rapamycin, a Food and Drug Administration-approved drug, may have applicability for use in creating a highly proliferative cell population for use in regenerative medicine.
Chemical Synthesis of U1 snRNA Derivatives (2013)

U1 snRNA is an interesting biological tool for splicing correction and regulation of gene expression. However, U1 snRNA has never been chemically synthesized. In this study, the first chemical synthesis of U1snRNA and its analogues was carried out. Moreover, it was found that the binding affinity of the modified U1 snRNA with an ethylene glycol linkage to snurportin 1 (nuclear import adaptor) was as high as that of the unmodified RNA.
The myocardial infarct-exacerbating effect of cell-free DNA is mediated by the high-mobility group box 1–receptor for advanced glycation end products–Toll-like receptor 9 pathway (2018)

Both HMGB1 and cfDNA are released from the heart upon reperfusion after prolonged ischemia and both contribute importantly and interdependently to post-IRI by a common RAGE-Toll-like receptor 9-dependent mechanism. Depleting either of these 2 damage-associated molecular patterns suffices to significantly reduce IS by approximately 50%.
Clinical efficacy of gene-modified stem cells in adenosine deaminase–deficient immunodeficiency (2017)

Food and Drug Administration Office of Orphan Product Development award, RO1 FD003005; NHLBI awards, PO1 HL73104 and Z01 HG000122; UCLA Clinical and Translational Science Institute awards, UL1RR033176 and UL1TR000124.
Design, Simulation, Fabrication, and Characterization of an Electrothermal Tip-Tilt-Piston Large Angle Micromirror for High Fill Factor Segmented Optical Arrays (2021)

Micro-electromechanical system (MEMS) micromirrors have been in development for many years, but the ability to steer beams to angles larger than 20° remains a challenging endeavor. This paper details a MEMS micromirror device capable of achieving large motion for both tip/tilt angles and piston motion. The device consists of an electrothermal actuation assembly fabricated from a carefully patterned multilayer thin-film stack (SiO<sub>2</sub>/Al/SiO<sub>2</sub>) that is epoxy bonded to a 1 mm<sup>2</sup> Au coated micromirror fabricated from an SOI wafer. The actuation assembly consists of four identical actuators, each comprised of a series of beams that use the inherent residual stresses and coefficient of thermal expansion (CTE) mismatches of the selected thin films to enable the large, upward, out-of-plane deflections necessary for large-angle beamsteering. Finite element simulations were performed (COMSOL v5.5) to capture initial elevations and tip/tilt motion displacements and achieved <10% variance in comparison to the experiment. The measured performance metrics of the micromirror include tip/tilt angles of ±23°, piston motion of 127 µm at sub-resonance, and dynamics characterization with observed resonant frequencies at ~145 Hz and ~226 Hz, for tip/tilt and piston motion, respectively. This unique single element design can readily be scaled into a full segmented micromirror array exhibiting an optical fill-factor >85%, making it suitable for optical phased array beam control applications.
Remarkable remission of a follicular lymphoma treated with rituximab and polychemotherapy (CHOP) (2003)

We describe a 50-year-old female patient who developed extensive lymphomatous infiltrates on her forehead, scalp and face within a few months. Histology and immunohistochemistry of skin tumours revealed a CD20 positive follicular B-cell lymphoma. Subsequently, extracutaneous manifestations were detected by computed tomography scans and bone marrow biopsy. The patient suffered from a primary nodular malignant lymphoma with extraordinary cutaneous infiltration of the head. Therefore, combination treatment with a monoclonal antibody against the CD20 antigen, rituximab, and polychemotherapy (CHOP scheme) was administered every 3 weeks. After the second course of treatment a complete regression of cutaneous infiltrates was noticed. Follow-up biopsies on the forehead showed no evidence of CD20 positive lymphoma cells, now. Despite mild leucocytopaenia therapy was well tolerated.
Fuel cells (2012)

Over the last two decades the problem of limited oil and gas supply versus new emerging-nation demands has created an immediate need for new technologies to alleviate global dependence on a hydrocarbon-based energy policy. This requires significant changes in the way global energy-system policy is managed and the rapid adoption/introduction of an array of new technologies that produce and use energy more efficiently and more cleanly than in the past. Specifically, these energy policy changes have directly led to more focus on commercial applications of alternative, sustainable energy policy. This chapter is centered on the establishment of a hydrogen-based economy, specifically as it relates to fuel cells. The net result is the concept of hydrogen and fuel cells as a practical foundation for implementing public policies responding to growing uncertainties about the security and long-term price of oil and environmental concerns. With the expectation that fuel cells and hydrogen can play a significant role in the global energy economy, governments are committing funds for research, development, and demonstration of hydrogen and fuel cells as they strive to create programs for viable infrastructure to support their use.
A Prototype of Transition-Metal-Catalyzed Carbothiolation of Alkynes (2001)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTA Prototype of Transition-Metal-Catalyzed Carbothiolation of AlkynesKunihiko Sugoh, Hitoshi Kuniyasu, Taeko Sugae, Atsushi Ohtaka, Yasutomo Takai, Aoi Tanaka, Chikako Machino, Nobuaki Kambe, and Hideo KurosawaView Author Information Department of Applied Chemistry, Faculty of Engineering Osaka University, Suita, Osaka 565-0871, Japan Cite this: J. Am. Chem. Soc. 2001, 123, 21, 5108–5109Publication Date (Web):May 2, 2001Publication History Received30 January 2001Published online2 May 2001Published inissue 1 May 2001https://doi.org/10.1021/ja010261oCopyright © 2001 American Chemical SocietyRIGHTS & PERMISSIONSArticle Views1427Altmetric-Citations119LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit Read OnlinePDF (47 KB) Get e-AlertsSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Aromatic compounds,Hydrocarbons,Palladium,Platinum,Vinyl Get e-Alerts
FAD dependent glucose dehydrogenases – Discovery and engineering of representative glucose sensing enzymes - (2019)

The history of the development of glucose sensors goes hand-in-hand with the history of the discovery and the engineering of glucose-sensing enzymes. Glucose oxidase (GOx) has been used for glucose sensing since the development of the first electrochemical glucose sensor. The principle utilizing oxygen as the electron acceptor is designated as the first-generation electrochemical enzyme sensors. With increasing demand for hand-held and cost-effective devices for the "self-monitoring of blood glucose (SMBG)", second-generation electrochemical sensor strips employing electron mediators have become the most popular platform. To overcome the inherent drawback of GOx, namely, the use of oxygen as the electron acceptor, various glucose dehydrogenases (GDHs) have been utilized in second-generation principle-based sensors. Among the various enzymes employed in glucose sensors, GDHs harboring FAD as the redox cofactor, FADGDHs, especially those derived from fungi, fFADGDHs, are currently the most popular enzymes in the sensor strips of second-generation SMBG sensors. In addition, the third-generation principle, employing direct electron transfer (DET), is considered the most elegant approach and is ideal for use in electrochemical enzyme sensors. However, glucose oxidoreductases capable of DET are limited. One of the most prominent GDHs capable of DET is a bacteria-derived FADGDH complex (bFADGDH). bFADGDH has three distinct subunits; the FAD harboring the catalytic subunit, the small subunit, and the electron-transfer subunit, which makes bFADGDH capable of DET. In this review, we focused on the two representative glucose sensing enzymes, fFADGDHs and bFADGDHs, by presenting their discovery, sources, and protein and enzyme properties, and the current engineering strategies to improve their potential in sensor applications.
Spontaneous and transient evoked otoacoustic emissions: A racial comparison (2001)

This study compared otoacoustic emissions (OAEs) between Chinese and Caucasian subjects. Two kinds of OAEs were measured: spontaneous otoacoustic emissions (SOAEs) and transient evoked otoacoustic emissions (TEOAEs). Both ears in 40 Chinese and 20 Caucasian young adults were examined. Within each racial group, equal numbers of normal hearing males and females were tested. Analysis of prevalence and amplitude distribution of both SOAEs and TEOAEs did not reveal any statistically significant racial differences. However, comparisons of the frequency distributions for both kinds of OAEs revealed significant findings between the two samples studied. Results showed that both SOAE and TEOAE measurements from Chinese subjects had comparatively higher responses in the high frequencies than those from Caucasian subjects. The findings are discussed in term of the reverse-transmission characteristics of the middle-ear conduction mechanism and/or possible cochlear differences between the two racial groups.
Understanding of the Influence of the Surface Defectivity on Silicon Heterojunction Cell Performance (2019)

The fabrication process of silicon heterojunction (SHJ) solar cells can induce locally depassivated regions (so-called defectivity) because of transportation steps (contact with belts, trays, etc) or simply the environment (presence of particles embedded within surface thin films). This surface passivation spatial heterogeneity is gaining interest as it may hinder the SHJ efficiency improvements allowed by incremental process steps optimizations. An experimentally-supported simulation study is proposed and applied on full size M2 SHJ cells in order to understand how the local a-Si:H/c-Si interface passivation loss impacts the overall cell performance. A simulation framework (developed on ATLAS Silvaco) was first validated through comparisons with experimental results. This step allowed then to use simulations results further to explore and understand the physics behind the defectivity-induced efficiency loss. The cell performance drop due to depassivated regions was attributed to a bias-dependent minority carrier recombination current flow towards the depassivated region, which is shown to affect mainly the Fill Factor.
FCR Provisions by Multi-Terminal HVDC System (2021)

Frequency control is considered to be an important function of multi-terminal HVDC (MTDC) systems. Sharing frequency containment reserves (FCRs) among interconnected AC systems using flexible HVDC technology is particularly attractive in the European context. However, coordination of the contribution between systems with different sizes, quality requirements and dynamic characteristics requires harmonized rules and well-defined control strategies. If the controllers are not properly designed, they can lead to disproportional supports, noncompliance with the existing regulation framework, or even degradation of the frequency quality. This paper analyzes several frequency control solutions for MTDC systems in terms of the adequacy of the expected contribution to the frequency containment process. The performance analyses are then confirmed using load-frequency models coupled with a three-terminal DC system. It is revealed that certain control solutions result in insufficient performance with respect to the desired FCR provision. The proposed distributed solution for each pair of converters can be the most compliant with the existing framework while keeping the highest degrees of freedom.
Work function engineering of doped Trilayer graphene grown on 4H-SiC (0001) (2015)

International audience
Carbon Nanotubes Reorganize Actin Structures in Cells and <i>ex Vivo</i> (2010)

The ability of globular actin to form filaments and higher-order network structures of the cytoskeleton is essential for cells to maintain their shape and perform essential functions such as force generation, motility, and division. Alterations of actin structures can dramatically change a cell's ability to function. We found that purified and dispersed single wall carbon nanotubes (SWCNTs) can induce actin bundling in cells and in purified model actin systems. SWCNTs do not induce acute cell death, but cell proliferation is greatly reduced in SWCNT-treated cells with an increase in actin-related division defects. Actin, normally present in basal stress fibers in control cells, is located in heterogeneous structures throughout the SWCNT-treated cell. These SWCNT-induced changes in actin structures are seen functionally in multinucleated cells and with reduced force generation. Ex vivo, purified actin filaments cross-linked with alpha-actinin and formed isotropic networks, whereas SWCNTs caused purified actin filaments to assemble into bundles. While purified, isolated SWCNTs do not appear acutely toxic, this subcellular reorganization may cause chronic changes to cellular functions.
Multi-color IRFPAs made from HgCdTe grown by MOVPE (2007)

The drive towards improved target recognition has led to an increasing interest in detection in more than one infrared band. This paper describes the design, fabrication and performance of two-colour and three-colour infrared detectors made from HgCdTe grown by Metal Organic Vapour Phase Epitaxy (MOVPE). The detectors are staring, focal plane arrays consisting of HgCdTe mesa-diode arrays bump bonded to silicon read-out integrated circuits (ROICs). Each mesa diode has one connection to the ROIC and the colours are selected by varying the applied bias. Results will be presented for both two-colour and three-colour devices. In a two-colour n-p-n design the cut-off wavelengths are defined by the compositions of the two n-type absorbers and the doping and composition of the p-type layer are chosen to prevent transistor action. The bias polarity is used to switch the output between colours. This design has been used to make MW/LW detectors with a MW band covering 3 to 5 &#956;m and a LW band covering 5 to 10 &#956;m. In a three-colour n-p-n design the cut-off wavelengths are defined by the compositions of the two n-type absorbers and the p-type absorber, which has an intermediate cut-off wavelength. The absorbers are separated from each other by electronic barriers consisting of wide band-gap material. At low applied bias these barriers prevent photo-electrons generated in the p-type absorber from escaping and the device then gives an output from one of the n-type absorbers. At high applied bias the electronic barrier is pulled down and the device gives an output from both the p-type absorber and one of the n-type absorbers. Thus by varying the polarity and magnitude of the bias it is possible to obtain three-colours from a two-terminal device. This design has been used to make a SW/MW/MW detector with cut-off wavelengths of approximately 3, 4 and 6 &#956;m.
Cancer theranosis using mono-disperse, mesoporous gold nanoparticles obtained via a robust, high-yield synthetic methodology (2016)

Here, we introduce the facile synthesis of scalable, mono-disperse, mesoporous gold nanoparticles (MPGNs) with an acidic emulsification method, which exhibit many attractive nanoplasmonic features for potential applications in many fields.
Variation of performance, of self-reported alertness and effort as a function of low doses of alcohol and of driving experience (2020)

Abstract Purpose Alcohol is the most frequent detected substance in the automobile fatal crashes but its precise mode of action on mental state is not always clear, notably when combined with lack of driving experience. The aim of this work is thus to evaluate performance and self-reported alertness and effort as a function of low doses of Blood Alcohol Concentration (BAC) and of drivers’ experience. Methods A single blind counterbalanced experiment was conducted on a driving simulator. Fifteen young novice drivers (18 years old, less of 2 months of driving licence) and fifteen young experienced drivers (21 years old, 3 years of driving licence) were tested with 0.0 g/l, 0.2 g/l and 0.5 g/l of alcohol at early afternoon. Driving scenarios was road tracking during 45 min at a constant speed of 110 km/h while maintaining a steady trajectory. Participants responded to the Thayer’s scale before and after each driving session, and to an adaptation of the NASA-TLX after each driving session. Statistical analyses were performed on driving parameters, self-reported alertness and effort as a function of alcohol level and driving experience. Results Alcohol deteriorated lateral and longitudinal stability of the trajectories. Notably with BAC 0.5 g/l, effort and speed increased but the increase in speed was particularly high for young experienced drivers. When young novice drivers felt that they were low alert, they also felt that they exerted more effort, their performance decreased, but they reduced their speed. Conclusions Alcohol thus degrades driving performance, and especially when the effort is high and alertness is low. The decrease of inhibitory process noted in presence of alcohol seems thus higher for young experienced than for young novice drivers. Results are in line with a drop in the blood alcohol level tolerated while driving.
A mechatronic platform for behavioral analysis on nonhuman primates (2012)

In this work we present a new mechatronic platform for measuring behavior of nonhuman primates, allowing high reprogrammability and providing several possibilities of interactions. The platform is the result of a multidisciplinary design process, which has involved bio-engineers, developmental neuroscientists, primatologists, and roboticians to identify its main requirements and specifications. Although such a platform has been designed for the behavioral analysis of capuchin monkeys (Cebus apella), it can be used for behavioral studies on other nonhuman primates and children. First, a state-of-the-art principal approach used in nonhuman primate behavioral studies is reported. Second, the main advantages of the mechatronic approach are presented. In this section, the platform is described in all its parts and the possibility to use it for studies on learning mechanism based on intrinsic motivation discussed. Third, a pilot study on capuchin monkeys is provided and preliminary data are presented and discussed.
Closed-loop modeling of the hippocampus and design of neurostimulation patterns for suppressing seizures (2013)

Anatomical and physiological evidence showing reciprocal connectivity between the hippocampus and Entorhinal Cortex, suggest that in tandem, they function as a closed-loop system. Using neuronal data recorded from the rodent hippocampal regions CA3 and CA1, we construct two open-loop input-output nonlinear dynamical models of the neural signal transformations from CA3 to CA1 and CA1 to CA3, and subsequently combine them into a closed-loop model. It was found that the closed-loop model replicates the theta and delta rhythms of the experimental data, whereas the open-loop models do not, suggesting closed-loop modeling is essential for representing the hippocampal activity. Furthermore, we have shown that the closed-loop model can generate strong oscillations akin to epileptic seizures and specific stimulation patterns can suppress these seizure-like oscillations. The latter result suggests that it may be possible to design model-based neurostimulation patterns to suppress seizure-like activity in the hippocampus.
SOX9 Regulates Multiple Genes in Chondrocytes, Including Genes Encoding ECM Proteins, ECM Modification Enzymes, Receptors, and Transporters (2014)

The transcription factor SOX9 plays an essential role in determining the fate of several cell types and is a master factor in regulation of chondrocyte development. Our aim was to determine which genes in the genome of chondrocytes are either directly or indirectly controlled by SOX9. We used RNA-Seq to identify genes whose expression levels were affected by SOX9 and used SOX9 ChIP-Seq to identify those genes that harbor SOX9-interaction sites. For RNA-Seq, the RNA expression profile of primary Sox9flox/flox mouse chondrocytes infected with Ad-CMV-Cre was compared with that of the same cells infected with a control adenovirus. Analysis of RNA-Seq data indicated that, when the levels of Sox9 mRNA were decreased more than 8-fold by infection with Ad-CMV-Cre, 196 genes showed a decrease in expression of at least 4-fold. These included many cartilage extracellular matrix (ECM) genes and a number of genes for ECM modification enzymes (transferases), membrane receptors, transporters, and others. In ChIP-Seq, 75% of the SOX9-interaction sites had a canonical inverted repeat motif within 100 bp of the top of the peak. SOX9-interaction sites were found in 55% of the genes whose expression was decreased more than 8-fold in SOX9-depleted cells and in somewhat fewer of the genes whose expression was reduced more than 4-fold, suggesting that these are direct targets of SOX9. The combination of RNA-Seq and ChIP-Seq has provided a fuller understanding of the SOX9-controlled genetic program of chondrocytes.
A Feasibility Governor for Enlarging the Region of Attraction of Linear Model Predictive Controllers (2020)

This paper proposes a method for enlarging the region of attraction of Linear Model Predictive Controllers (MPC) when tracking piecewise-constant references in the presence of pointwise-in-time constraints. It consists of an add-on unit, the Feasibility Governor (FG), that manipulates the reference command so as to ensure that the optimal control problem that underlies the MPC feedback law remains feasible. Offline polyhedral projection algorithms based on multi-objective linear programming are employed to compute the set of feasible states and reference commands. Online, the action of the FG is computed by solving a convex quadratic program. The closed-loop system is shown to satisfy constraints, be asymptotically stable, exhibit zero-offset tracking, and display finite-time convergence of the reference.