gebhart/patrepeval huggingface dataset:
[Impact on tumor angiogenesis and tumor progression of expression of the 18 kd and 24 kd isoforms of FGF-2]. (1999)

The role of FGF-2 in tumor progression and tumor cell invasiveness was investigated using the rat bladder carcinoma cells NBT-II, which do not constitutively express FGF-2 or its membrane-spanning receptor. The NBT-II cells were transfected using expression vectors encoding either the 18 kD or the 24 kD isoform of FGF-2. The 24 kD isoform contains a nuclear localization signal. The transfected NBT-II cells that expressed 18 kD FGF-2 produced and secreted this factor as the biologically active form and retained an epithelial morphology. When injected to nude mice, the tumorigenic potential of these cells was not increased over that of non-transfected NBT-II cells; however, although the time to tumor development was long, the tumors were highly vascularized, indicating secretion of the angiogenic factor FGF-2. The transfected NBT-II cells that expressed 24 kD FGF-2 varied in their morphological appearance and did not secrete FGF-2; immunofluorescence and Western-blot studies showed that the FGF-2 was mainly intranuclear. When injected to nude mice, these cells produced tumors and migrated not only to the lymph nodes but also to the lungs where they produced metastases. In aggregate, these data indicate that stimulation of angiogenesis is not sufficient to increase tumor growth and that nuclear FGF-2 acts as a tumorigenic and metastasis-promoting factor in the NBT-II carcinoma model.
Knockout‐Based Switches (2006)

This chapter contains sections titled: Single-Stage Knockout Switch Channel Grouping Principle Two-Stage Multicast Output-Buffered ATM Switch (MOBAS) Appendix
Femoroazetabuläres Impingement-Syndrom bei Adoleszenten – Wie beraten? Wie behandeln? (2022)

FAIS in adolescents primarily affects physically active patients. Certain sports favor the development of FAIS. Cam impingement, pincer impingement, and combined FAIS are the most common entities in this age group. Cam morphology occurs shortly before closure of the proximal femoral growth plate. In cam impingement, the slipped capital femoral epiphysis (SCFE) must be distinguished from the primary cam morphology. SCFE requires rapid surgical treatment with stabilization of the epiphysis, while primary cam impingement can be analyzed electively, and conservative treatment is first recommended. Damage to the labrum and cartilage is regularly observed. A systematic radiological evaluation using X‑rays and MRI is mandatory in order to develop an adequate treatment plan. In adolescent patients with FAIS, a conservative attempt at therapy should always be made; if this is unsuccessful, surgical repair with hip arthroscopy is indicated. The postoperative results are very good in adolescents, with a rapid improvement in symptoms, few complications, and a high return-to-sport rate.
A wave-number domain approach to the active control of structure-borne sound (1991)

Previous work has shown the possibilities of active control of structurally radiated sound by applying control forces directly to the structure while error information is taken from microphones placed in the radiated far field is impracticable in many applications. On the other hand, it has been demonstrated that simply minimizing the structural response, i.e., by using accelerometers as sensors, may lead to an increase in the radiated sound levels due to “control spillover.” Thus, this research work demonstrates for the first time the design of feed-forward controller in the wave-number domain for eliminating the use of microphones in the radiated far field. By using a stationary phase approach to evaluate sound radiation from panels, it is shown that only one spectral wave-number component of the structural response radiates towards one particular angle. Thus, by minimizing particular wave-number components on the structure, the sound radiation can be minimized at the corresponding radiation angles. A 2-D baffled simply supported beam example demonstrates this concept. [Work supported by ONR/DARPA.]
Draft Response to the Internet Coordination Group Request for Proposals on IANA (2014)

This document contains the a draft response to a request for proposals
from the IANA Stewardship Transition Coordination Group regarding the
protocol parameters registries. It is meant to be included in an
aggregate proposal that also includes contributions covering names and
addresses that will be submitted from their respective operational
communities. The IETF community is invited to comment and propose
changes to this document.
Low-frequency Raman excitations in phase I of solid<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mrow><mml:msub><mml:mrow><mml:mi mathvariant="normal">H</mml:mi></mml:mrow><mml:mrow><mml:mn>2</mml:mn></mml:mrow></mml:msub></mml:mrow><mml:mo>:</mml:mo></mml:math>Role of crystal fields (2001)

Low-frequency Raman spectra (up to $800{\mathrm{cm}}^{\ensuremath{-}1})$ measured below 18 K are reported for the entire pressure range of phase I of para-hydrogen doped with low fractions of the ortho-modification. In addition to bands corresponding to the ${E}_{2g}$ optical phonon and the $J=2\ensuremath{\leftarrow}0$ rotational transitions, we observed a weak band attributed as ${S}_{0}(1).$ These spectra are employed to deduce information on the interactions that play a decisive role in bringing about the transition to the broken symmetry phase II. A new theoretical analysis is presented to understand the rotational Raman excitations and the role of various pairwise anisotropic interactions between ${\mathrm{H}}_{2}$ molecules. In order to explain the pressure dependence of the ${S}_{0}(0)$ and ${S}_{0}(1)$ bands, we infer that a fourth-harmonic crystal field is present. The frequencies of the ${S}_{0}(0)$ and ${S}_{0}(1)$ bands are used to reconstruct the respective crystal field parameter.
High-resolution x-ray reflectivity study of thin layered Pt-electrodes for integrated ferroelectric devices (2001)

The structural interface properties of layered Pt/Ti/SiO2/Si electrodes have been investigated using high-resolution specular and diffuse x-ray reflectivity under grazing angles. Currently this multilayer system represents a technological standard as bottom electrodes for ferroelectric thin-film applications. For the electronic and ferroelectric properties of integrated devices, the film-electrode interface is of crucial importance. We focused on Pt 1000 Å/Ti 100 Å/SiO2/Si electrodes prepared under annealing conditions as employed in industrial processing, prior to the deposition of ferroelectric films. The comparison between annealed and non-annealed electrodes clearly revealed strong interfacial effects due to interdiffusion and oxidation of Ti, especially at the Pt-Ti interface. Migration of Ti into the Pt layer results in a shift of the critical angle due to the enclosure of TiO2-x within the Pt layer. The heterogeneous distribution of TiO2-x suggests a diffusion mechanism mainly along the Pt grain boundaries. At the SiO2 interface a relatively weakly oxidized Ti layer of 20 Å remained, which is most probably correlated with the remaining adhesion to the substrate.
G704 Numerical Simulation of the Unsteady Cavitating Flow in an Inducer for Turbopump(2) (2006)

The unsteady cavitating flow in a turbopump inducer has been calculated using an accurate three-dimensional detached eddy simulation (DES) code, which took into account the cavitating phenomena. The three-dimensional code is based on the numerical method for compressible flow using low Mach number flow approximation and a cavitation model. Qualitative agreement between our computational result and experimental data was satisfactory. The magnitude of the fluctuation in cavity volume increased as the cavities extend and close to the throat of the blade passage. The effect of the tip-gap on the cavity volume was shown; the time-averaged volume of cavities for the inducer with the tip-gap was about five times larger than that for the inducer without the tip-gap at an operating condition. Vorticity generation in cavities due to baroclinic torque was found. Multiple blade passages calculations are under way to find unsteady phenomena such as rotating cavitation.
A generic approach for producing various protrusive shapes on different size scales using shape-memory polymer (2007)

We demonstrate a generic approach for producing different shaped and sized protrusive features using shape-memory polymer (SMP). Utilizing the large recoverable strain in the SMP, and depending on the pre-straining of the SMP, shape of the indenter, temperature during indenting and the depth of polishing, we show that a variety of features of different sizes can be easily obtained.
Extraction of Acids and Bases from Aqueous Phase to a Pseudoprotic Ionic Liquid (2019)

We report experiments on the extraction of acids and bases from an aqueous phase to a pseudoprotic ionic liquid phase consisting of an equimolar mixture of trihexylamine and octanoic acid. We observed the extraction of a wide range of acids and bases, and investigated the mechanism of extraction in detail. Our results confirmed the observation of the Hofmeister effect in these systems reported in our previous work, where the extent of the extraction of copper salts was significantly influenced by the interactions between extracted inorganic anions and the organic phase. Our results further demonstrated that the organic layer served as a "floating buffer" capable of stabilizing the pH of an acidic or alkaline aqueous phase. The results tie current interest in protic and pseudoprotic ionic liquids to earlier work on the extraction of acids using amine and acid⁻base couples as extraction agents in an inert organic solvent.
Isolation of a beta-tubulin gene from Fusarium moniliforme that confers cold-sensitive benomyl resistance (1996)

A beta-tubulin gene from a UV-irradiated benomyl-resistant mutant of Fusarium moniliforme was isolated, cloned, and sequenced. The gene encodes a 446-amino-acid polypeptide with homology to other fungal beta-tubulins. RNA blot analysis showed expression of the gene during vegetative growth and conidial germination but no expression during conidiation. A point mutation, which likely confers benomyl resistance, has been identified in the cloned gene; this mutation results in a single amino acid substitution of asparagine for tyrosine at position 50. Expression of benomyl resistance in the mutant was also cold sensitive. Sexual crosses betweeen the mutant and a wild-type strain indicated cosegregation of benomyl resistance and cold sensitivity.
Chapitre 24 : Comprendre la lésion médullaire traumatique et ses conséquences motrices. Implications en recherche fondamentale et clinique. (2011)

National audience
Adaptive distributed estimation over wireless sensor networks with packet losses (2007)

A distributed adaptive algorithm to estimate a time-varying signal, measured by a wireless sensor network, is designed and analyzed. The presence of measurement noises and of packet losses is considered. Each node of the network locally computes adaptive weights that guarantee to minimize the estimation error variance. Decentralized conditions on the weights, which ensure the stability of the estimates throughout the overall network, are also considered. A theoretical performance analysis of the scheme is carried out both in the presence of perfect and lossy links. Numerical simulations illustrate performance for various network topologies and packet loss probabilities.
Interventions for bullous pemphigoid (2005)

Very potent topical steroids are effective and safe treatments for bullous pemphigoid; their use in extensive disease may be limited by side effects and practical factors. Starting doses of prednisolone greater than 0.75 mg/kg/day do not seem to give additional benefit, lower doses may be adequate for disease control; this could reduce the incidence and severity of adverse reactions. The effectiveness of the addition of plasma exchange or azathioprine to corticosteroids has not been established. Combination treatment with tetracycline and nicotinamide may be useful; this needs further validation.
An RNA Ligase from Deinococcus radiodurans (2004)

Although DNA repair pathways have been the focus of much attention, there is an emerging appreciation that distinct pathways exist to maintain or manipulate RNA structure in response to breakage events. Here we identify an RNA ligase (DraRnl) from the radiation-resistant bacterium Deinococcus radiodurans. DraRnl seals 3'-OH/5'-PO4 RNA nicks in either a duplex RNA or an RNA: DNA hybrid, but it cannot seal 3'-OH/5'-PO4 DNA nicks. The specificity of DraRnl arises from a requirement for RNA on the 3'-OH side of the nick. DraRnl is a 342-amino acid monomeric protein with a distinctive structure composed of a C-terminal adenylyltransferase domain linked to an N-terminal module that resembles the OB-fold of phenylalanyl-tRNA synthetases. RNA sealing activity was abolished by mutation of the predicted lysine adenylylation site (Lys-165) in the C-terminal domain and was reduced by an order of magnitude by deletion of the N-terminal OB module. Our findings highlight the existence of an RNA repair capacity in bacteria and support the hypothesis that contemporary DNA ligases, RNA ligases, and RNA capping enzymes evolved by the fusion of ancillary effector domains to an ancestral catalytic module involved in RNA repair.
Observations on Sound Propagation in Rapidly Rotating Bose-Einstein Condensates (2005)

Repulsive laser potential pulses applied to vortex lattices of rapidly rotating Bose-Einstein condensates create propagating density waves which we have observed experimentally and modeled computationally to high accuracy. We have observed a rich variety of dynamical phenomena ranging from interference effects and shock-wave formation to anisotropic sound propagation.
Geographically Distinct Escherichia coli O157 Isolates Differ by Lineage, Shiga Toxin Genotype, and Total Shiga Toxin Production (2014)

While the differential association of Escherichia coli O157 genotypes with animal and human hosts has recently been well documented, little is known about their distribution between countries and how this might affect regional disease rates. Here, we used a 48-plex single nucleotide polymorphism (SNP) assay to segregate 148 E. coli O157 isolates from Australia, Argentina, and the United States into 11 SNP lineages. We also investigated the relationship between SNP lineages, Shiga toxin (Stx) gene profiles, and total Stx production. E. coli O157 isolates clearly segregated into SNP lineages that were differentially associated with each country. Of the 11 SNP lineages, seven were detected among isolates from a single country, two were detected among isolates from all three countries, and another two were detected only among U.S. and Argentinean isolates. A number of Australian (30%) and Argentinean (14%) isolates were associated with novel, previously undescribed SNP lineages that were unique to each country. Isolates within SNP lineages that were strongly associated with the carriage of stx2a produced comparatively more Stx on average than did those lacking the stx2a subtype. Furthermore, the proportion of isolates in stx2a-associated SNP lineages was significantly higher in Argentina and the United States than Australia (P < 0.05). This study provides evidence for the geographic divergence of E. coli O157 and for a prominent role of stx2a in total Stx production. These results also highlight the need for more comprehensive studies of the global distribution of E. coli O157 lineages and the impacts of regionally predominant E. coli O157 lineages on the prevalence and severity of disease.
Near-Infrared Metatronic Nanocircuits by Design (2013)

Lumped circuit elements (i.e., resistors, capacitors, and inductors) provide the basic building blocks of microelectronic devices ubiquitous in information processing, storage, and communications. The use of these modular quasistatic components can be extended to the nanoscale optical regime to achieve high-density, high-speed analogues of these traditional circuits. We reimagine these devices in the near-infrared (NIR) regime, making use of a simple nanorod geometry and plasmonic transparent conducting oxides (TCOs). We evaluate their equivalent impedance as lumped circuit elements and construct bandpass and band-stop filters operating at NIR wavelengths. Through variation in the TCO nanorod geometry and the addition of PbS nanocrystals in between and NiCr on top of the TCO nanorods, we present the first designable NIR lumped nanocircuits with tailorable response. The experimental results agree with both circuit models and full-wave simulations.
Synthetic Lethal Interaction of SHOC2 Depletion with MEK Inhibition in RAS-Driven Cancers (2019)

The mitogen-activated protein kinase (MAPK) pathway is a critical effector of oncogenic RAS signaling, and MAPK pathway inhibition may be an effective combination treatment strategy. We performed genome-scale loss-of-function CRISPR-Cas9 screens in the presence of a MEK1/2 inhibitor (MEKi) in KRAS-mutant pancreatic and lung cancer cell lines and identified genes that cooperate with MEK inhibition. While we observed heterogeneity in genetic modifiers of MEKi sensitivity across cell lines, several recurrent classes of synthetic lethal vulnerabilities emerged at the pathway level. Multiple members of receptor tyrosine kinase (RTK)-RAS-MAPK pathways scored as sensitizers to MEKi. In particular, we demonstrate that knockout, suppression, or degradation of SHOC2, a positive regulator of MAPK signaling, specifically cooperated with MEK inhibition to impair proliferation in RAS-driven cancer cells. The depletion of SHOC2 disrupted survival pathways triggered by feedback RTK signaling in response to MEK inhibition. Thus, these findings nominate SHOC2 as a potential target for combination therapy.
AhOJ Ceramics Made by CAM-LEM (Computer-Aided Manufacturing Engineering Materials) Technology (2018)

The concept ofCAM-LEM technology is presented and discussed in the context ofthe&#13;\nfabrication ofAh03 ceramics. Particular attention is paid to the interplay ofgreen tape&#13;\ncharacteristics and the unit operations involved in CAM-LEM. Examples of ceramic shapes&#13;\ndifficult to form by conventional methods are described.
Practical Asymmetric Synthesis of (<i>S</i>)-4-Ethyl-7,8-dihydro-4-hydroxy-1<i>H</i>-pyrano[3,4-f]indolizine- 3,6,10(4<i>H</i>)-trione, a Key Intermediate for the Synthesis of Irinotecan and Other Camptothecin Analogs (1997)

A practical asymmetric synthesis of (S) 4-ethyl-7,8-dihydro-4-hydroxy-1H-pyrano[3,4-f]indolizine-3,6,10(4H)-trione (1), a versatile intermediate for the synthesis of camptothecin analogs, was developed. Commercially available citrazinic acid is converted in four steps into the 2-chloro-6-methoxypyridine 5. An ortho-directed metalation followed by reaction with a formamide produces an aldehyde with the required 2,3,4,6-substituted pyridine (6) with high regioselectivity. After refunctionalization of the aldehyde, the chloropyridine is converted into an ester by a facile palladium-mediated carbonylation reaction. Wittig reaction and racemic osmylation produce the diol 16 which is resolved by an efficient lipase resolution to an ee > 99%, and a one-pot recycle of the unwanted diol enantiomer was developed. A series of high-yielding oxidation and deprotection steps convert (S)-16 into the pyridone 25, which is then converted into 1 with an ee > 99.6%.
Online Minimum-Jerk Trajectory Generation (2015)

Robotic trajectory generation is reformulated as a controller design problem. For minimum-jerk trajectories, an optimal controller using the Hamilton-Jacobi-Bellman equation is derived. The controller instantaneously updates the trajectory in a closed-loop system as a result of changes in the reference signal. The resulting trajectories coincide with piece-wise fifth-order polynomial trajectories for piece-wise constant target states. Since having hard constraints on the final time poses certain robustness issues, a smooth transition between the finite-horizon and an infinite-horizon problem is developed. This enables to switch softly to a tracking mode when a moving target is reached.
Freestanding Laser-Induced Graphene Ultrasensitive Resonative Viral Sensors (2022)

Early and reliable detection of an infectious viral disease is critical to accurately monitor outbreaks and to provide individuals and health care professionals the opportunity to treat patients at the early stages of a disease. The accuracy of such information is essential to define appropriate actions to protect the population and to reduce the likelihood of a possible pandemic. Here, we show the fabrication of freestanding laser-induced graphene (FLIG) flakes that are highly sensitive sensors for high-fidelity viral detection. As a case study, we show the detection of SARS-CoV-2 spike proteins. FLIG flakes are nonembedded porous graphene foams ca. 30 μm thick that are generated using laser irradiation of polyimide and can be fabricated in seconds at a low cost. Larger pieces of FLIG were cut forming a cantilever, used as suspended resonators, and characterized for their electromechanics behavior. Thermomechanical analysis showed FLIG stiffness comparable to other porous materials such as boron nitride foam, and electrostatic excitation showed amplification of the vibrations at frequencies in the range of several kilo-hertz. We developed a protocol for aqueous biological sensing by characterizing the wetting dynamic response of the sensor in buffer solution and in water, and devices functionalized with COVID-19 antibodies specifically detected SARS-CoV-2 spike protein binding, while not detecting other viruses such as MS2. The FLIG sensors showed a clear mass-dependent frequency response shift of ∼1 Hz/pg, and low nanomolar concentrations could be detected. Ultimately, the sensors demonstrated an outstanding limit of detection of 2.63 pg, which is equivalent to as few as ∼5000 SARS-CoV-2 viruses. Thus, the FLIG platform technology can be utilized to develop portable and highly accurate sensors, including biological applications where the fast and reliable protein or infectious particle detection is critical.
Online magnetic calibration of a cutting edge 9-axis wireless Inertial Measurement Unit (2012)

In the last years there has been increasing interest on Inertial Measurement Units (IMUs) in several fields, because they can provide a measurement of the orientation, velocity, and acceleration at low cost with high performance. The main reason of the low cost is the possibility to find inertial sensors, as accelerometers, gyroscopes and magnetometers, based on Micro-Electro-Mechanical Systems (MEMS) made by silicon, with further advantages of small dimensions and mass production from the main semiconductor suppliers. In this paper, we present the development of the ultra miniaturized wireless IMU, WB-4 (Waseda Bioinstrumentation number 4), with a 3-axis accelerometer, 3-axis gyroscope and 3-axis magnetometer. Additionally, we illustrate, together with results, the algorithms used for the data filtering and calibration, with particular emphasis on a new methodology for the online calibration of the digital magnetometer.
ESCRTing DNA at the Cleavage Site During Cytokinesis (2012)

During cell division, Aurora B prevents collisions between chromatin and the cytokinetic apparatus by targeting the ESCRT-III subunit CHMP4C.