gebhart/patrepeval huggingface dataset:
3114 Investigating the therapeutic potential of parthenolide in the treatment of hematopoietic neoplasms in dogs (2019)

OBJECTIVES/SPECIFIC AIMS: Determine PTL’s mechanism(s) of action in a panel of canine hematopoietic cell lines; this will enable us to 1) verify that PTL is working as expected and 2) rationally select combination therapeutics. Characterize the in vitro sensitivity of canine hematopoietic cell lines to PTL in combination with other chemotherapeutic agents. Determine immunohistochemical NFκB expression in tissue microarrays of spontaneous canine neoplasms and correlate with outcome-linked data. Characterize the in vivo sensitivity of canine hematopoietic cell lines to PTL using a murine xenograft model. METHODS/STUDY POPULATION: Growth inhibition assays were performed using a panel of canine mast cell, histiocytic sarcoma, lymphoma, and leukemia cell lines, with PTL alone or in combination with redox-perturbing standard-of-care therapeutics. Cell death was assessed using flow cytometry. Immunofluorescence and immunoblotting were used to assess NFκB localization and phosphorylation of NFκB p65 (transcriptional activation), respectively. Intracellular glutathione with and without PTL and combination chemotherapeutics will be assessed spectrophotometrically. Archived spontaneous canine tumors will be evaluated immunohistochemically (IHC) for increased NFκB pathway activation relative to normal control tissues. Nude mice will receive intravenous, intraperitoneal, or subcutaneous injections of canine HS cells and will be treated with PTL or with PTL in combination with standard-of-care chemotherapeutics. RESULTS/ANTICIPATED RESULTS: Results: All immortalized canine cell lines evaluated are sensitive to PTL therapy and undergo dose-dependent apoptosis following exposure to drug. PTL exposure leads to inhibition of NFκB, as evidenced by immunofluorescent nuclear exclusion and decreased p65 phosphorylation. Some chemotherapeutics appear to synergize with PTL in vitro. Anticipated results: We expect to find increased IHC NFκB pathway activation in malignantly transformed tissues relative to controls. We expect standard-of-care therapeutics to synergize with PTL in vivo based on preliminary in vitro data. DISCUSSION/SIGNIFICANCE OF IMPACT: These studies will determine whether PTL therapy may be beneficial in dogs with a variety of hematopoietic neoplasms, either alone or in combination with other therapeutics that are currently in clinical use. Dogs with mast cell or histiocytic neoplasia are an excellent model for rare and deadly human diseases, which may also benefit from PTL therapy.
Effects of hydrophilic polymer-coated polysulfone membrane dialyzers on intradialytic hypotension in diabetic hemodialysis patients (ATHRITE BP Study): a pilot study (2017)

Intradialytic hypotension (IDH) is a common clinical manifestation associated with poor prognosis in hemodialysis (HD) patients. HD patients who suffer from diabetic nephropathy (DN) are increasing and diabetes is a major cause of IDH. Effective interventional treatments for IDH have yet to be fully evaluated. The aim of this multicenter prospective study is to clarify the effect of biocompatible hydrophilic polymer-coated polysulfone (PS) membrane, TORAYLIGHT® NV (NV) dialyzers on IDH. This is a prospective stratified-randomized multicenter trial. Forty DN patients undergoing HD and receiving two or more times of treatments for IDH per month were enrolled in this study. They were stratified by the number of treatments for IDH and divided to two groups using NV or conventional PS/polyethersulfone (PES) dialyzers. The number of treatments for IDH and changes in systolic blood pressure (SBP) were monitored for 6 months. Patients’ demographic and clinical characteristics were also collected at enrollment and the last month of the observation period. In order to clarify the patient characteristics that induced preferable effects by using NV dialyzers, responders were defined as the patients whose average SBP falls in 1 month improved from over 30 mmHg to no more than 30 mmHg. The total number of treatments for IDH decreased significantly in NV group, even though pre-dialysis body weight and ultrafiltration volume were similar. In addition, patients using NV had significantly higher post-dialysis SBP and the lowest SBP during HD at sixth month compared as those in PS/PES group. NV responders had valuables suggesting malnutrition and microinflammation, and better lipid profiles than non-responders. However, the representative markers related to nutritional status, arteriosclerosis, and inflammation were not improved by NV treatment. NV had preferable effects on IDH in DN HD patients. Our results suggest the usefulness of NV as a possible method to deal with IDH. Further studies are needed to clarify the mechanism of NV effects on hemodynamic status. This study was registered on September 26, 2013, in the University Hospital Medical Information Network-Clinical Trials Registry in Japan (Study ID: UMIN000011872 ).
Piezovoltaics from PdH<sub><i>x</i></sub> (2023)

Metal hydrides have wide applications in energy science. A large pressure gradient propels the hydrogen atoms out. A piezovoltaic device, a pressure gradient-driven battery, can therefore be realized when the migrations of protons and electrons are separated by different conductors. Here we investigate the piezovoltaic performance of PdH<sub><i>x</i></sub> with various proton conductors as electrolytes and experimentally detect an output current of ≲40 nA and a voltage of ∼0.8 V for a 3 μg sample. We also demonstrate the escape of hydrogen atoms from a palladium lattice under an increasing pressure gradient using X-ray diffraction. The relationship between piezovoltaics (chemical process) and piezoelectricity (physical process) is like that between a chemical battery and a capacitor. Our work demonstrates the piezovoltaic application of metal hydrides and provides a new way to convert mechanical energy into electrical energy.
Modelling and Validation of Tooth Tip Leakages in Gerotor Pumps (2019)

Abstract Gerotor pumps are commonly used as charge pumps in hydrostatic transmission systems as well as in fuel injection and automotive lubrication systems. For high pressure applications, Gerotors suffer from internal leakages which are one of the main sources of volumetric loss. The curvature of typical gear profiles for Gerotors prevents the usage of simple analytical relations to describe this leakage flow. In this paper, a non-dimensional functional relationship is developed between geometric and flow parameters to model this leakage flow. A set of CFD simulations are conducted on the tooth tip geometry to establish this relationship. Then, an experimental setup is designed to reproduce the flow conditions at tooth tip of Gerotor. Experiments are conducted for a range of geometric and flow parameters and results from experiments are used to validate the proposed non-dimensional model. The tooth tip leakage model developed and validated in this work is valuable for pump designers in determining the impact of gear geometry and clearances on volumetric performance of the pump. Moreover, the model can be readily used in any lumped parameter based simulation tool permitting a fast and accurate prediction of the tooth tip leakage flow and hence the volumetric efficiency of the unit.
On the Energy Cost of Channel Based Key Agreement (2016)

Besides security, energy consumption is a major concern for devices in the Internet of Things (IoT). We compare the energy consumption of two key agreement schemes -- Channel-Based Key Agreement (CBKA) and Elliptic Curve Diffie-Hellman (ECDH) -- in the IoT setting, using Wi-Fi as wireless communication interface. While ECDH is a well-studied protocol, CBKA has received attention only in recent years. Several publications proposed CBKA as a low-energy alternative to ECDH, but they did not address the energy cost of communication. For a fair comparison, we implemented the schemes on a 32-bit ARM Cortex M3-based IoT platform and measured the respective energy consumption for computation and communication. Our results show that the limiting factor for CBKA over Wi-Fi is the energy cost of communication, in particular the cost of acquiring the Received Signal Strength Indicator (RSSI) values. Even in an optimal scenario, CBKA must not measure more than ca. 300 RSSI values to be more energy efficient than ECDH. This is at most 1/5 of RSSI values required by CBKA implementations reported in the literature. As an optimization, we present a refined CBKA protocol which can save up to 25% of the energy compared to existing protocols by exploiting inherent data exchanges for entropy extraction.
Department of Error (2019)

GBD 2017 Risk Factor Collaborators. Global, regional, and national comparative risk assessment of 84 behavioural, environmental and occupational, and metabolic risks or clusters of risks for 195 countries and territories, 1990–2017: a systematic analysis for the Global Burden of Disease Study 2017. Lancet 2018; 392: 1923–94—In this Global Health Metrics paper, affiliations have been amended for Yasin Jemal Yasin, Joseph Adel Mattar Banoub, and Tanuj Kanchan; the declaration of interests statement has been amended for Boris Bikbov; and acknowledgments have been added for providers of smoking relative risk data.
Glycoside Hydrolase Catalysis: Do Substrates and Mechanism-Based Covalent Inhibitors React via Matching Transition States? (2022)

In this study, we look at how a catalytically efficient α-galactosidase stabilizes transition state (TS) charge delocalization for substrate hydrolysis. We then assess whether covalent inhibition of the enzyme by three types of mechanism-based covalent inhibitors occurs via similar modes of TS stabilization. We show, using Bartlett-type linear free energy relationships, that good correlations are obtained between the catalytic efficiencies (kcat/Km and/or kinact/Ki) for enzyme-catalyzed reactions of natural and activated galactoside substrates and of representatives of three families of classical mechanism-based inhibitors: a 2-deoxy-2-fluoroglycoside, allylic carbasugars, and an epoxy carbasugar. Of note, we show that glycoside natural substrates and allylic carbasugars display log(rate)–log(rate) correlations that are unity (slope ≈ 1), an observation consistent with them having identical positive charge stabilization at the SN1-like glycosylation and pseudo-glycosylation TSs, respectively. In contrast, 2-deoxy-2-fluoroglycoside mechanism-based inhibitors react via a different enzyme-catalyzed mechanism (SN2), while the strained epoxy carbasugar inactivates the α-galactosidase by traversing a TS in which the glycoside hydrolase stabilizes the inactivation TS that has a significantly lower degree of charge stabilization to those for the natural glycoside substrates. To add weight to these conclusions, we computed free energy landscapes and their associated galactosylation and pseudo-galactosylation TSs using QM/MM molecular dynamics methods with the whole solvated enzyme.
C09 Measurements of acoustic minor losses in the tapered tube (2012)

Thermoacoustic devices are composed of tubes whose radii are usually different each other. There are two joint conditions of the tubes: they are jointed directly or via a tapered tube. In this study, acoustically lost power was measured at the joint position under the two conditions.
Effective Treatment of Acute Promyelocytic Leukemia With All-<i>Trans</i>-Retinoic Acid, Arsenic Trioxide, and Gemtuzumab Ozogamicin (2008)

The combination of ATRA and ATO (with or without GO) as initial therapy for APL was effective and safe and can substitute chemotherapy-containing regimens.
FEBio: History and Advances (2017)

The principal goal of the FEBio project is to provide an advanced finite element tool for the biomechanics and biophysics communities that allows researchers to model mechanics, transport, and electrokinetic phenomena for biological systems accurately and efficiently. In addition, because FEBio is geared toward the research community, the code is designed such that new features can be added easily, thus making it an ideal tool for testing novel computational methods. Finally, because the success of a code is determined by its user base, integral goals of the FEBio project have been to offer support and outreach to our community; to provide mechanisms for dissemination of results, models, and data; and to encourage interaction between users. This review presents the history of the FEBio project, from its initial developments through its current funding period. We also present a glimpse into the future of FEBio.
Influenza Virus Neuraminidase Inhibitory Activity of Phlorotannins from the Edible Brown Alga <i>Ecklonia cava</i> (2011)

Influenza A virus infections continue to pose a major threat to humans and several animal species. Neuraminidase (NA) is one of the most promising targets for the development of drugs against influenza viruses because of its critical role in the viral life cycle. During the course of a search for NA inhibitors from edible natural sources, we found that the ethyl acetate layer of ethanol extracts of Ecklonia cava showed extremely high NA-inhibitory activity (72.1% inhibition at 30 μg/mL). Bioactivity-guided fractionation of the ethyl acetate layer yielded five phlorotannins, identified as phloroglucinol (1), eckol (2), 7-phloroeckol (3), phlorofucofuroeckol (4), and dieckol (5). The inhibitory activities of these compounds (1-5) against NAs from group-1 (A/Bervig_Mission/1/18 [H1N1], A/PR/8/34 [H1N1]) and group-2 (A/Hong Kong/8/68 [H3N2], A/Chicken/Korea/MS96/96 [H9N2]) influenza A were evaluated to determine potencies and kinetic behavior. Analyses using various in vitro influenza A virus NA assays showed that all five phlorotannin derivatives were selective NA inhibitors. Of the phlorotannins, phlorofucofuroeckol (4) exhibited the most potent inhibitory activities toward group-1 NAs (IC₅₀ values, 4.5 and 14.7 μM), whereas dieckol (5) potently inhibited group-2 NAs. Kinetic analyses indicated that compounds 1-5 were all noncompetitive. Notably, these noncompetitive inhibitors synergized with oseltamivir to enhance the NA-inhibitory effects of oseltamivir.
Unobtrusive Long-Range Detection of Passive RFID Tag Motion (2006)

This paper presents a novel method for detecting the motion of passive radio-frequency-identification (RFID) tags within the field of a detecting antenna. The method allows the unobtrusive detection of human interactions with RFID-tagged objects without requiring any modifications to existing communications protocols or RFID hardware. We use the response rate (a metric in lieu of the true received RF-signal intensity) at the reader to study the impact of tag translation, rotation, and coupling, as well as environmental effects. Performance is improved by introducing the idea of multiple tags/readers. Movement-detection algorithms are developed and integrated into the RFID monitoring system, and verified by experiments that demonstrate excellent results.
Metal-Directed Assembly of Triple-Layered Fluorescent Metallocyclophanes (1999)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTMetal-Directed Assembly of Triple-Layered Fluorescent MetallocyclophanesBradley J. Holliday, Joshua R. Farrell, Chad A. Mirkin, Kin-Chung Lam, and Arnold L. RheingoldView Author Information Department of Chemistry, 2145 Sheridan Road Northwestern University, Evanston, Illinois 60208 Department of Chemistry and Biochemistry University of Delaware, Newark, Delaware 19716 Cite this: J. Am. Chem. Soc. 1999, 121, 26, 6316–6317Publication Date (Web):June 19, 1999Publication History Received12 April 1999Published online19 June 1999Published inissue 1 July 1999https://pubs.acs.org/doi/10.1021/ja991140fhttps://doi.org/10.1021/ja991140frapid-communicationACS PublicationsCopyright © 1999 American Chemical SocietyRequest reuse permissionsArticle Views764Altmetric-Citations64LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Chemical structure,Fluorescence,Macrocycles,Metals,Molecules Get e-Alerts
Cutting Edge: Serum but Not Mucosal Antibody Responses Are Associated with Pre-Existing SARS-CoV-2 Spike Cross-Reactive CD4+ T Cells following BNT162b2 Vaccination in the Elderly (2022)

Advanced age is a main risk factor for severe COVID-19. However, low vaccination efficacy and accelerated waning immunity have been reported in this age group. To elucidate age-related differences in immunogenicity, we analyzed human cellular, serological, and salivary SARS-CoV-2 spike glycoprotein-specific immune responses to the BNT162b2 COVID-19 vaccine in old (69-92 y) and middle-aged (24-57 y) vaccinees compared with natural infection (COVID-19 convalescents, 21-55 y of age). Serological humoral responses to vaccination excee-ded those of convalescents, but salivary anti-spike subunit 1 (S1) IgA and neutralizing capacity were less durable in vaccinees. In old vaccinees, we observed that pre-existing spike-specific CD4<sup>+</sup> T cells are associated with efficient induction of anti-S1 IgG and neutralizing capacity in serum but not saliva. Our results suggest pre-existing SARS-CoV-2 cross-reactive CD4<sup>+</sup> T cells as a predictor of an efficient COVID-19 vaccine-induced humoral immune response in old individuals.
Intracellular targeting of protein kinase C isoenzymes: functional implications (1995)

Conference Article| August 01 1995 Intracellular targeting of protein kinase C isoenzymes: functional implications S. C. Kiley; S. C. Kiley ‡ *W. Alton Jones Cell Science Center, Inc., 10 Old Barn Road, Lake Placid, NY 12946, U.S.A. ‡To whom correspondence is to be addressed Search for other works by this author on: This Site PubMed Google Scholar S. Jaken; S. Jaken *W. Alton Jones Cell Science Center, Inc., 10 Old Barn Road, Lake Placid, NY 12946, U.S.A. Search for other works by this author on: This Site PubMed Google Scholar R. Whelan; R. Whelan †Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, P.O. Box 123, 44 Lincoln's Inn Fields, London WC2A 3PX, U.K. Search for other works by this author on: This Site PubMed Google Scholar P. J. Parker P. J. Parker †Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, P.O. Box 123, 44 Lincoln's Inn Fields, London WC2A 3PX, U.K. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1995) 23 (3): 601–605. https://doi.org/10.1042/bst0230601 Article history Received: March 03 1995 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation S. C. Kiley, S. Jaken, R. Whelan, P. J. Parker; Intracellular targeting of protein kinase C isoenzymes: functional implications. Biochem Soc Trans 1 August 1995; 23 (3): 601–605. doi: https://doi.org/10.1042/bst0230601 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: CSK, cytoskeleton, MAP, micro-tubule-associated protein, MARCKS, myristoylated alanine-rich C-kinase substrate, PKC, protein kinase C, PMA, phorbol 12-myristate 13-acetate, PS, phosphatidyl-serine This content is only available as a PDF. © 1995 Biochemical Society1995 Article PDF first page preview Close Modal You do not currently have access to this content.
Copper Toxicity to the Ghost Rams-Horn Snail <i>Biomphalaria havanensis</i> (2022)

Abstract Avian piscivores cause direct economic losses to the aquaculture industry through predation, as well as indirect losses through transmission of digenetic trematodes. Bolbophorus damnificus is a trematode parasite associated with significant losses in catfish aquaculture. The complex life cycle involves the American white pelican Pelecanus erythrorhynchos, planorbid snails, and ictalurid catfish. With no approved therapeutics for B. damnificus in catfish Ictalurus spp. and federal protections on American white pelican, management is restricted to controlling snail hosts. Two snail species, marsh rams-horn snail Planorbella trivolvis and ghost rams-horn snail Biomphalaria havanensis, are common inhabitants of commercial catfish ponds and known to transmit B. damnificus. Previous work evaluated copper sulfate toxicity on marsh rams-horn snails; however, data are lacking for ghost rams-horn snails. Herein, laboratory-reared adults of ghost rams-horn snails and marsh rams-horn snails were exposed to copper concentrations ranging from 0.16 to 3.38 mg/L Cu to evaluate 24-, 48-, and 72-h acute toxicity. Additionally, sequential low-dose treatments ranging from 0.1 to 0.8 mg/L Cu were evaluated on eggs, juveniles, and adults of ghost rams-horn snails as a potentially safer treatment regime for ponds. Acute toxicity was similar between snail species for all exposure times. A 72-h exposure produced an LC50 of 0.10 and 0.37 mg/L Cu for ghost rams-horn snails and marsh rams-horn snails, respectively. The LC50 values increased to 1.1 mg/L Cu for both species with 48-h exposure. However, in the multiple low-dose study, a single dose of 0.4 or 0.8 mg/L Cu was lethal to all adult snails after 1 week, as was 0.2 mg/L Cu after two doses. Four doses of 0.1 mg/L Cu or greater killed all ghost rams-horn snail juveniles. Results indicate that marsh rams-horn snails and ghost rams-horn snails have similar sensitivity to copper, and multiple low-dose treatments were effective against all life stages of ghost rams-horn snails. These data indicate that copper can be an effective treatment for snail control in commercial catfish ponds.
Editorial Comments (1990)

No AccessJournal of Urology1 Jan 1990Editorial Comments William R. Fair William R. FairWilliam R. Fair More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)54830-2AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "Editorial Comments." The Journal of Urology, 143(1), p. 40 © 1990 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 143Issue 1January 1990Page: 40 Advertisement Copyright & Permissions© 1990 by The American Urological Association Education and Research, Inc.MetricsAuthor Information William R. Fair More articles by this author Expand All Advertisement PDF downloadLoading ...