gebhart/patrepeval huggingface dataset:
Abstract 110: The role of the p53 target Wig-1 in senescence and cancer (2015)

Abstract The wild type p53-induced gene 1, Wig-1 (also known as Zmat3 or PAG608) binds to AU-rich elements in mRNAs and thereby regulates important tumor associated factors including p53, FAS, and N-Myc. Focusing on normal human diploid fibroblasts (HDF), we are now exploring the role of Wig-1 in senescence. SiRNA-mediated Wig-1 depletion increases senescence markers such as Beta-galactosidase staining, H3K9me3, H4K20me3, and p21. Also, Wig-1 is spontaneously downregulated in cells undergoing replicative senescence. The trimethylation of lysine 9 on histone 3 (H3K9me3) is an established marker of cellular senescence and increases upon siRNA-mediated Wig-1 knockdown in HDF cells. It is also increased in late passage (senescent) fibroblasts that express low Wig-1 levels. Our RNA-immunoprecipitation sequencing (RIP-seq) data of tumor cells shows that Wig-1 binds histone-modifying enzymes SUV39H2 (which trimethylates H3K9), and subunits of PP1 (protein phosphatase 1, which dephosphorylates H3T10). These mRNAs have AU-rich elements in the 3´UTR. Importantly, we have observed that both SUV39H2 and its corresponding methylation (H3K9me3) is increased upon Wig-1 silencing. In a cohort of 350 lung cancer patients, we found Wig-1 gene amplification in a large portion of squamous cell carcinomas, whereas in lung adenocarcinomas the Wig-1 gene was unchanged. Interestingly, strong Wig-1 staining in this cohort correlates to worse prognosis. We think Wig-1 attenuates senescence via downregulating important senescence markers in both tumor and normal cells. Evidence point to Wig-1 playing an inhibitory role on the mRNAs of histone modifying enzymes, thereby acting on well established senescence markers of gene expression and chromatin regulation. Citation Format: Fredrik Jerhammar, Cinzia Bersani, Dijana Djureinovic, Patrick Micke, Klas G. Wiman. The role of the p53 target Wig-1 in senescence and cancer. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 110. doi:10.1158/1538-7445.AM2015-110
Planar defects in massively transformed Ti-Al alloys (1999)

The microstructure of massively transformed Ti-Al alloys has been studied by conventional transmission electron microscopy and high-resolution electron microscopy (HREM). A high density of planar defects, namely complex antiphase domain boundaries (CAPDBs) and thermal microtwins (TMTs) have been observed. Consistent with previous diffraction contrast studies, HREM confirmed that two antiphase-related gamma-matrix domains are generally separated by a thin layer of a 90o domain, for which the c axis is rotated through 90o over a common cube axis with respect to those of the gamma-matrix domains. Two crystallographically distinct types of 90o domains have been observed in association with the CAPDBs. Furthermore, interactions between the CAPDBs and TMTs have been investigated. In regions where TMTs intersect the generally morphologically wavy noncrystallographic CAPDBs, the latter have been observed to facet parallel to the {111}-twinning planes of the TMTs. This local interaction between CAPDBs and TMTs is associated with the transfer of true twinning shears of the type 1/6<112] from the matrix to the thin 90o domain generating a pseudotwinned region where the corresponding shears are of the type 1/6<121]. Diffusion associated with the local rearrangement of the atomic stacking is expected for the accommodation of the relaxation of the CAPDBs by facetting on the low-energy {111} planes. Possible mechanisms for the genesis of these complex planar defects are discussed.
Efficient and effective cellular technology: Femtocells (2016)

Around 70% of the mobile calls and 90% of the data service requests are generated inside the buildings. Therefore resources are being utilized by indoor subscribers in large quantity and this promotes operators will to provide satisfactory indoor services. High data rates for the data services to indoor consumers. Information Communication & Technologies (ICT) community has significantly increased the energy requirement and toxics like Carbon dioxide and carbon monoxide emissions, which are altering the environmental conditions and causing energy loss. Femtocells came into existence to resolve these problems in indoors. This paper has detailed description on Femtocells. The wide range of applications of femtocells in homes and enterprises shows that these provide efficient coverage and speed to provide better network capacity in indoors. The deployment cost of such kind of devices is very less. Telecom companies have offered femtocells to approach these problems in the form of cellular technology. Femtocells facilitate better indoor mobile call coverage and high speed facility. In this work, the architecture of femtocells and other technical matters related to Femtocells and their management system are described.
A Practical Approach to Language Complexity: A Wikipedia Case Study (2012)

In this paper we present statistical analysis of English texts from Wikipedia. We try to address the issue of language complexity empirically by comparing the simple English Wikipedia (Simple) to comparable samples of the main English Wikipedia (Main). Simple is supposed to use a more simplified language with a limited vocabulary, and editors are explicitly requested to follow this guideline, yet in practice the vocabulary richness of both samples are at the same level. Detailed analysis of longer units (n-grams of words and part of speech tags) shows that the language of Simple is less complex than that of Main primarily due to the use of shorter sentences, as opposed to drastically simplified syntax or vocabulary. Comparing the two language varieties by the Gunning readability index supports this conclusion. We also report on the topical dependence of language complexity, that is, that the language is more advanced in conceptual articles compared to person-based (biographical) and object-based articles. Finally, we investigate the relation between conflict and language complexity by analyzing the content of the talk pages associated to controversial and peacefully developing articles, concluding that controversy has the effect of reducing language complexity.
An Approach to Patients with Endometriosis (1988)

University of Arkansas Little Rock, Arkansas University of Cincinnati College of Medicine Cincinnati, Ohio
The Seven Challenges of LED Lighting (2016)

This paper focuses on some of the most important issues that Power Designers tackle when designing power conversion systems for LED lighting. Approaches and proposals to address them in terms of products, technology, control methodologies and power system architecture will be presented. Finally, looking ahead, an outline of how some of these challenges - and the relevant responses - are expected to evolve in the near future will be given.
T-cell infiltration after chronic constriction injury of mouse sciatic nerve is associated with interleukin-17 expression and secondary macrophage recruitment (2006)

Aims: Cytokine networks are involved in adaptive responses after peripheral nervous system injury. While it is well established that macrophages play a decisive role in nerve degeneration after chronic constriction injury (CCI) the role of T-cells is not yet well defined. Interleukin (IL)-17 is a novel proinflammatory cytokine produced by activated T-cells, but not macrophages.
Simple and Highly Sensitive Molecular Diagnosis of Zika Virus by Lateral Flow Assays (2016)

We have developed a simple, user-friendly, and highly sensitive Zika virus (ZIKV) detection method by incorporating optimized reverse transcription loop-mediated isothermal amplification (RT-LAMP) and a lateral flow assay (LFA). The optimized RT-LAMP reaction was carried out using Bst 3.0 polymerase, which has robust and fast isothermal amplification performance even in the presence of high concentrations of inhibitors; this permitted the amplification of ZIKV RNA in pure water and human whole blood. In addition, the strong reverse transcription activity of Bst 3.0 polymerase enabled specific ZIKV RNA amplification without extra addition of reverse transcriptase. The RT-LAMP condition was optimized by adjusting the Mg<sup>2+</sup> and dNTP mix concentration to extirpate nontarget amplification, which is caused by nonspecific primer dimers amplification. After 30 min of RT-LAMP reaction, the resultant amplicons were simply and rapidly analyzed by the LFA test in less than 5 min. The optimized RT-LAMP combined with the LFA allowed specific ZIKV RNA detection down to the single copy level within 35 min.
PROSPECTIVE ASSESSMENT OF PATIENT REPORTED URINARY CONTINENCE AFTER RADICAL PROSTATECTOMY (2000)

Reported urinary continence rates after radical prostatectomy vary. Although modifications of radical prostatectomy meant to improve outcome, such as nerve sparing or bladder neck preservation, are in widespread use, to our knowledge evidence to support these practices based on patient report is scant. We evaluated the potential effects of nerve sparing and bladder neck preservation on urinary continence after radical prostatectomy, and assessed the impact of various urinary continence definitions on the observed outcome.We prospectively evaluated a cohort of men with prostate cancer who elected surgery with and without nerve sparing, and bladder neck preservation as primary therapy. A total of 482 men completed a brief urinary continence questionnaire preoperatively and postoperatively at a median followup of 18 months. Urinary continence was followed prospectively using the questionnaire and patient reported urinary continence recovery was based on 3 definitions of continence.Median time to continence recovery based on patient reporting was significantly shorter in the nerve sparing than in the nonnerve sparing group when continence was defined as no urinary leakage (5.3 versus 10.9 months, p <0.01). A multivariate model controlling for baseline factors revealed that significant predictors of continence outcome were preoperative continence, patient age, nerve sparing and the interaction of nerve sparing with age (p <0.05). The definition of urinary continence also affected outcome.The nerve sparing technique of radical prostatectomy was associated with improved recovery of urinary continence in an age dependent manner, whereas bladder neck preservation was not beneficial. Patient age and the sensitivity of the incontinence definitions, as reflected by the associated variable rates of preoperative baseline incontinence, are significant contexts for interpreting urinary function data after radical prostatectomy. These factors may partially explain the variation in continence rates in the literature.
Man-made target backscattering behavior: applicability of conventional radar resolution theory (1996)

Radar is not achieving its potential performance. The reasons for this are discussed. The backscattering behavior of man-made targets is much more complicated than conventionally modeled. This makes the conventional resolution theory inappropriate when man-made targets are highly resolved, with the consequence that conventional signal processing and image analysis techniques miss much of the information in the radar returns. The type of backscattering behavior we have observed with real data, and how it affects resolution performance is discussed here. It is also shown that one must analyze the complex image rather than the intensity image.
Measurement of Cortisol and Testosterone in Hair of Obese and Non-Obese Human Subjects (2014)

Hair cortisol levels increase, while hair testosterone levels decrease with obesity. The hair C/T ratio was significantly correlated with age, BMI and waist circumference better than hair cortisol or testosterone alone. As hair collection is non-invasive and is not influenced by moment-to-moment variations, the measurement of hormones in hair is a useful tool in research and possibly clinical practice.
Impaired Hippocampal Synaptic Plasticity and Enhanced Excitatory Transmission in a Novel Animal Model of Autism Spectrum Disorders with Telomerase Reverse Transcriptase Overexpression. (2018)

Recently, we have reported that animals with telomerase reverse transcriptase (TERT) overexpression exhibit reduced social interaction, decreased preference for novel social interaction and poor nest-building behaviors symptoms that mirror those observed in human autism spectrum disorders (ASD). Overexpression of TERT also alters the excitatory/inhibitory (E/I) ratio in the medial prefrontal cortex. However, the effects of TERT overexpression on hippocampal-dependent learning and synaptic efficacy have not been investigated. In the present study, we employed electrophysiological approaches in combination with behavioral analysis to examine hippocampal function of TERT transgenic (TERT-tg) mice and FVB controls. We found that TERT overexpression results in enhanced hippocampal excitation with no changes in inhibition and significantly impairs long-term synaptic plasticity. Interestingly, the expression levels of phosphorylated CREB and phosphory-lated CaMKIIα were significantly decreased while the expression level of CaMKIIα was slightly increased in the hippocampus of TERT-overexpressing mice. Our observations highlight the importance of TERT in normal synaptic function and behavior and provide additional information on a novel animal model of ASD associated with TERT overexpression.
Robust Second-Order Blind Equalization Of Polyphase Channels (1998)

Publication in the conference proceedings of EUSIPCO, Rhodes, Greece, 1998
The association between preterm newborn hypotension and hypoxemia and outcome during the first year (1993)

Ninety-eight newborn infants, less than 34 weeks at birth, were studied to examine the relationship between newborn hypotension and hypoxemia and brain damage. Heart rate, blood pressure and oxygen tension were recorded continuously during the 96 h following delivery. Outcome measures included neuropathology in children who died, and motor and cognitive development at one year corrected age in children who survived. There were 22 children with a minor and 27 with a major abnormal outcome. There was a relationship between newborn hypotension, newborn hypoxemia and low birth weight, and a major abnormal outcome. The probability of a major abnormal outcome increased from 8% in newborns with no hypotension or hypoxemia, to 53% in children with both hypotension and hypoxemia. These findings support the contention that combinations of sustained newborn hypotension and hypoxemia are important factors in the development of brain damage, accounting for a major abnormal outcome.
Improved integrated circuits qualification using Dynamic Laser Stimulation techniques (2009)

Dynamic laser stimulation techniques have been developed with success for failure analysis of integrated circuit subjected to ldquosoft defectrdquo. Weakness in design and physical defect can be isolated and located through these methodologies for digital, analog and mixed mode devices. But they are now successfully used embedded in qualification process since they provide accurate information about the device robustness and weaknesses evolutions. Efficiency of this approach has been demonstrated on an EEPROPM.
Guest Editorial—Selected Papers from the IEEE International Mixed-Signals, Sensors, and Systems Test Workshop (IMS3TW), 2008 (2009)

This special section includes papers that were presented during the IEEE International Mixed-Signals, Sensors, and Systems Test Workshop (IMS3TW) 2008 and extended into full papers for this issue.
SPOILER: Speculative Load Hazards Boost Rowhammer and Cache Attacks (2019)

Modern microarchitectures incorporate optimization techniques such as speculative loads and store forwarding to improve the memory bottleneck. The processor executes the load speculatively before the stores, and forwards the data of a preceding store to the load if there is a potential dependency. This enhances performance since the load does not have to wait for preceding stores to complete. However, the dependency prediction relies on partial address information, which may lead to false dependencies and stall hazards. 
In this work, we are the first to show that the dependency resolution logic that serves the speculative load can be exploited to gain information about the physical page mappings. Microarchitectural side-channel attacks such as Rowhammer and cache attacks like Prime+Probe rely on the reverse engineering of the virtual-to-physical address mapping. We propose the SPOILER attack which exploits this leakage to speed up this reverse engineering by a factor of 256. Then, we show how this can improve the Prime+Probe attack by a 4096 factor speed up of the eviction set search, even from sandboxed environments like JavaScript. Finally, we improve the Rowhammer attack by showing how SPOILER helps to conduct DRAM row conflicts deterministically with up to 100% chance, and by demonstrating a double-sided Rowhammer attack with normal user's privilege. The later is due to the possibility of detecting contiguous memory pages using the SPOILER leakage.
New Generation 10kV SiC Power MOSFET and Diodes for Industrial Applications (2015)

For the first time, we present the full characteristics of a newer generation 10kV, 340mOmega SiC MOSFET and 10kV, 15A SiC diode chip set and discuss target applications. The 8.1mm x 8.1mm 10kV SiC MOSFET die was measured to have record low specific RDSON of 100mOmega- cm2 at 25deg C, 15A current rating, and only 6.5mJ total switching energy when switched at 6kV, 15A, 150deg C. The accompanying anti-parallel 10kV diode chip has the same die size, a 25% reduction from previous efforts. This new class of 10kV SiC die is targeted for several new applications, including solid state transformers utilizing a 7.2kV or 4.16kV line-voltage.
Development of a prototype gantry system for preclinical x‐ray phase‐contrast computed tomography (2011)

The concept of grating-based phase-contrast computed tomography (CT) can be implemented into a compact, cone-beam geometry gantry setup. The authors believe that this work represents an important milestone in translating phase-contrast x-ray imaging from previous proof-of-principle experiments to first preclinical biomedical imaging applications on small-animal models.
Shape of diffraction orders of centered and decentered pixelated lenses (2010)

We consider diffraction by pixelated lenses when the lens size is significantly smaller than the diffraction pattern of single pixels. In that case, the diffraction orders show shapes that have not been identified in earlier studies and that are quite sensitive to the pixel filling ratio as well as to decentering.
Computational Offloading for Energy Constrained Devices in Multi-Hop Cooperative Networks (2019)

As the number of mobile devices that natively support ad hoc communication protocols increase, large ad hoc networks can be created not only to facilitate communication among the mobile devices, but also to assist devices that are executing computationally intensive applications. Prior work has developed computation offloading systems for mobile devices, but this work has focused exclusively on offloading to single hop neighbors, due in part to the practical challenges of setting up multi-hop networks using existing ad hoc communication protocols. However, limiting the offloading of computation to one-hop neighbors inherently restricts the number of devices that can participate in the distributed computation. By presenting a heuristic, aimed at avoiding partitioning the network, as well as an iterative task assignment algorithm that can optimize the assignment of computational tasks to devices in a multi-hop cooperative network, we are able to evaluate the effect of computational offloading in multi-hop networks. Experimental results, obtained from an implementation on Android devices, are integrated with an analytical model that enables the evaluation of system performance under a variety of conditions. These experimental and analytic results demonstrate the benefit of enabling computation offloading to all devices in a multi-hop cooperative network.
In Vivo Hepatic Adenoviral Gene Delivery Occurs Independently of the Coxsackievirus–Adenovirus Receptor (2002)

Systemic administration of adenoviral vectors leads to a widespread distribution of vector. Therefore, targeting of adenoviral vectors to specific tissues or cell types will require methods to ablate the normal tropism of the vector simultaneously with the introduction of new receptor specificities. To inhibit native receptor binding, we mutated residues in the AB loop of the adenovirus type 5 (Ad5) fiber. We genetically incorporated the S408E-P409A mutation, referred to as KO1, into the adenoviral genome alone or in combination with an RGD-targeting ligand in the HI loop of fiber. Transduction experiments confirmed that the KO1 mutation results in a significant reduction in fiber-dependent gene transfer on A549 and primary fibroblast cells that could be restored via the RGD-targeting ligand. Competition transduction experiments verified the receptor-binding properties of each vector on A549 and hepatocytes in vitro. Unexpectedly, in mice systemic delivery of the vector containing the KO1 mutation resulted in efficient liver transduction that was localized specifically to hepatocytes. We confirmed these results in three different mouse strains, indicating that hepatic adenoviral gene transfer may be independent of the coxsackievirus-adenovirus receptor and that in vivo retargeting will require further viral capsid modifications to generate a fully detargeted adenoviral vector upon which to introduce new tropisms.
Quantifying Cell‐Derived Changes in Collagen Synthesis, Alignment, and Mechanics in a 3D Connective Tissue Model (2022)

Abstract Dysregulation of extracellular matrix (ECM) synthesis, organization, and mechanics are hallmark features of diseases like fibrosis and cancer. However, most in vitro models fail to recapitulate the three‐dimensional (3D) multi‐scale hierarchical architecture of collagen‐rich tissues and as a result, are unable to mirror native or disease phenotypes. Herein, using primary human fibroblasts seeded into custom fabricated 3D non‐adhesive agarose molds, a novel strategy is proposed to direct the morphogenesis of engineered 3D ring‐shaped tissue constructs with tensile and histological properties that recapitulate key features of fibrous connective tissue. To characterize the shift from monodispersed cells to a highly‐aligned, collagen‐rich matrix, a multi‐modal approach integrating histology, multiphoton second‐harmonic generation, and electron microscopy is employed. Structural changes in collagen synthesis and alignment are then mapped to functional differences in tissue mechanics and total collagen content. Due to the absence of an exogenously added scaffolding material, this model enables the direct quantification of cell‐derived changes in 3D matrix synthesis, alignment, and mechanics in response to the addition or removal of relevant biomolecular perturbations. To illustrate this, the effects of nutrient composition, fetal bovine serum, rho‐kinase inhibitor, and pro‐ and anti‐fibrotic compounds on ECM synthesis, 3D collagen architecture, and mechanophenotype are quantified.