gebhart/patrepeval huggingface dataset:
Molecular misreading in non‐neuronal cells (2000)

+1 Frame-shifted proteins such as amyloid precursor protein(+1) and ubiquitin-B(+1) have been identified in the neuropathological hallmarks of Alzheimer's disease. These frameshifts are caused by dinucleotide deletions in GAGAG motifs of messenger RNA encoded by genes that have maintained the unchanged wild-type DNA sequence. This process is termed 'molecular misreading'. A key question is whether this process is confined to neurons or whether it could also occur in non-neuronal cells. A transgenic mouse line (MV-B) carrying multiple copies of a rat vasopressin minigene as a reporter driven by the MMTV-LTR promotor was used to screen non-neuronal tissues for molecular misreading by means of detection of the rat vasopressin(+1) protein and mutated mRNA. Molecular misreading was demonstrated to occur in several organs (e.g., epididymis and the parotid gland) where transgenic vasopressin expression is abundant, but its penetrance is variable both between and within tissues. This implies that non-neural tissues too, could be affected by cellular derangements caused by molecular misreading.
Optimizing cloud resources for delivering IPTV services through virtualization (2012)

Virtualized cloud-based services can take advantage of statistical multiplexing across applications to yield significant cost savings. However, achieving similar savings with real-time services can be a challenge. In this paper, we seek to lower a provider's costs for real-time IPTV services through a virtualized IPTV architecture and through intelligent time-shifting of selected services. Using Live TV and Video-on-Demand (VoD) as examples, we show that we can take advantage of the different deadlines associated with each service to effectively multiplex these services. We provide a generalized framework for computing the amount of resources needed to support multiple services, without missing the deadline for any service. We construct the problem as an optimization formulation that uses a generic cost function. We consider multiple forms for the cost function (e.g., maximum, convex and concave functions) reflecting the cost of providing the service. The solution to this formulation gives the number of servers needed at different time instants to support these services. We implement a simple mechanism for time-shifting scheduled jobs in a simulator and study the reduction in server load using real traces from an operational IPTV network. Our results show that we are able to reduce the load by ~ 24% (compared to a possible ~ 31%). We also show that there are interesting open problems in designing mechanisms that allow time-shifting of load in such environments.
Colorectal Cancer Epidemiology in the Nurses’ Health Study (2016)

The NHS has provided new insights into colorectal adenomas, CRC etiology, and pathogenic mechanisms. With its unique strengths, the NHS should continue to contribute to the field of CRC epidemiology and play a major role in public health.
Circuit models for Bulk Current Injection (BCI) clamps with multiple cables (2018)

The Bulk Current Injection (BCI) method is widely used for RF immunity testing of automotive systems. If detected at a late stage, compliance failures may lead to expensive redesigns and increased time to market. Therefore, it is desirable to evaluate the chip immunity at an early design stage using simulation methods. In general, the 3D physical model of the BCI clamp is not easily accessible from the manufacturer. Moreover, modeling the clamp along with the device-under-test (DUT) may lead to large simulation times. An accurate circuit model of the clamp-cable configuration will enable the designer simulate BCI performance through early design iterations. Although, there are circuit models reported in literature for clamps with a single cable available in literature, they do not directly lend themselves to multiple cable modeling. In this paper, a circuit modeling methodology is proposed for a BCI injection clamp with multiple cables. The proposed method is validated with measurements.
Pneumatic microsyringe for use as an injector in open tubular liquid chromatography and as a dispenser in microanalysis (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPneumatic microsyringe for use as an injector in open tubular liquid chromatography and as a dispenser in microanalysisRobert T. Kennedy and James W. JorgensonCite this: Anal. Chem. 1988, 60, 15, 1521–1524Publication Date (Print):August 1, 1988Publication History Published online1 May 2002Published inissue 1 August 1988https://pubs.acs.org/doi/10.1021/ac00166a009https://doi.org/10.1021/ac00166a009research-articleACS PublicationsRequest reuse permissionsArticle Views107Altmetric-Citations17LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Fibrous proteins: At the crossroads of genetic engineering and biotechnological applications (2015)

Fibrous proteins, such as silk, elastin and collagen are finding broad impact in biomaterial systems for a range of biomedical and industrial applications. Some of the key advantages of biosynthetic fibrous proteins compared to synthetic polymers include the tailorability of sequence, protein size, degradation pattern, and mechanical properties. Recombinant DNA production and precise control over genetic sequence of these proteins allows expansion and fine tuning of material properties to meet the needs for specific applications. We review current approaches in the design, cloning, and expression of fibrous proteins, with a focus on strategies utilized to meet the challenges of repetitive fibrous protein production. We discuss recent advances in understanding the fundamental basis of structure-function relationships and the designs that foster fibrous protein self-assembly towards predictable architectures and properties for a range of applications. We highlight the potential of functionalization through genetic engineering to design fibrous protein systems for biotechnological and biomedical applications.
Epitope-Specific Regulatory CD4 T Cells Reduce Virus-Induced Illness while Preserving CD8 T-Cell Effector Function at the Site of Infection (2010)

The role of epitope-specific regulatory CD4 T cells in modulating CD8 T-cell-mediated immunopathology during acute viral infection has not been well defined. In the murine model of respiratory syncytial virus (RSV) infection, CD8 T cells play an important role in both viral clearance and immunopathology. We have previously characterized two RSV epitope-specific CD4 T-cell responses with distinct phenotypic properties. One of them, the IA(b)M(209)-specific subset, constitutively expresses FoxP3 and modulates CD8 T-cell function in vitro. We show here that the IA(b)M(209)-specific CD4 T-cell response regulates CD8 T-cell function in vivo and is associated with diminished RSV-induced illness without affecting viral clearance at the site of infection. Achieving the optimal balance of regulatory and effector T-cell function is an important consideration for designing future vaccines.
Effect of Initiation Conditions on the Uniformity of Three-Arm Star Molecular Brushes (2003)

A series of three-arm star densely grafted molecular brushes with poly(n-butyl acrylate) side chains were prepared by atom transfer radical polymerization (ATRP). The three-arm macroinitiators of poly(2-bromopropionyloxyethyl methacrylate) were prepared by ATRP of 2-trimethylsilyloxyethyl methacrylate using trifunctional initiators 1,1,1-tris(4-(2-bromoisobutyryloxy)phenyl)ethane and different catalytic systems, i.e., a CuIX/dNbpy complex (X = Br, Cl) with and without CuIIX2 (X = Br, Cl) as deactivators, followed by the subsequent esterification with 2-bromopropionyl bromide. The kinetics exhibited first-order reaction with respect to the monomer concentration in all systems. Polymerization rates decreased with the catalyst in the order of CuBr > CuBr/CuBr2 > CuCl > CuCl/CuCl2. The different initiation conditions also affect the polydispersities of the final brushes, although in all cases the number-average molecular weight (Mn) increases with the increasing monomer conversion. AFM measurements of multiarm brushes enabled quantitative analysis of the length distribution. Because of visualization of individual starlike molecules, one could separately analyze the brush arms and the whole molecule. The polydispersities of the arm length decreased in the order of CuBr > CuBr/CuBr2 > CuCl > CuCl/CuCl2. The CuCl/CuCl2 system seems to be the best catalyst for the star synthesis yielding brushes with narrow length distribution. CuBr is a relative "worst" catalyst system in which several coupled molecular brushes were observed, while some arms were missing. These led to the broadest distribution of arm length. Similar analysis for four-arm molecular brushes was performed. The polydispersity of the total length was significantly lower than those of the arm length. Here, the total length polydispersity displayed amazing agreement with Schulz−Flory theory for chain coupling.
Mechanism of binding of multivalent immune complexes to Fc receptors. 1. Equilibrium binding (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMechanism of binding of multivalent immune complexes to Fc receptors. 1. Equilibrium bindingSteven K. Dower, Charles DeLisi, Julie A. Titus, and David M. SegalCite this: Biochemistry 1981, 20, 22, 6326–6334Publication Date (Print):October 1, 1981Publication History Published online1 May 2002Published inissue 1 October 1981https://pubs.acs.org/doi/10.1021/bi00525a007https://doi.org/10.1021/bi00525a007research-articleACS PublicationsRequest reuse permissionsArticle Views243Altmetric-Citations58LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
cardano: R function to calculate the real roots of a cubic polynomial (2014)

R function to calculate the real roots of the cubic polynomial R function to calculate the real roots of the cubic polynomial ax^3+bx^2+cx+d = 0. It uses the so-called Cardano's formula (first derived by Scipione del Ferro, not Cardano).
Feature based object recognition using statistical occlusion models with one-to-one correspondence (2002)

In this paper we present a new Bayesian framework for partially occluded object recognition with one-to-one correspondence. We introduce two different statistical models for occlusion: One model assumes that each feature in the model can be occluded independent of whether any other features are occluded, whereas the second model uses spatially correlated occlusion to represent the extent of occlusion. Using these models, the object recognition problem reduces to finding the object hypothesis with largest generalized likelihood We develop fast algorithms for finding the optimal one-to-one correspondence between scene features and object model features to compute the generalized likelihood. We evaluate our algorithms using examples extracted from synthetic aperture radar imagery, and illustrate the performance advantages of our approach over alternative algorithms proposed by others.
General planar quadrilateral mesh design using conjugate direction field (2011)

We present a novel method to approximate a freeform shape with a planar quadrilateral (PQ) mesh for modeling architectural glass structures. Our method is based on the study of conjugate direction fields (CDF) which allow the presence of ±κ/4(κ ε Z) singularities. Starting with a triangle discretization of a freeform shape, we first compute an as smooth as possible conjugate direction field satisfying the user's directional and angular constraints, then apply mixed-integer quadrangulation and planarization techniques to generate a PQ mesh which approximates the input shape faithfully. We demonstrate that our method is effective and robust on various 3D models.
Electrophoretic Deposition of Carbon Nanotubes onto Zinc Substrates for Electrode Applications (2020)

Carbon nanotubes (CNTs) as nanostructured materials have been widely used to improve electrochemical performance of electrode materials for various batteries and electrolyzers. The purpose of this work was to investigate the electrophoretic deposition (EPD) of multi-walled CNTs (MWCNTs) onto Zn plates for application in aqueous Zn ion batteries. The effects of MWCNTs on Zn oxidation and reduction were assessed using cyclic voltammetry. Before EPD, the MWCNTs were modified using H 2 SO 4 /HNO 3 under reflux to improve dispersion stability in water. Acid modification shortened the MWCNTs but did not cause significant changes in crystallinity, tube diameter, and interlayer spacing. In a second step, the acid modified MWCNTs were homogeneously deposited onto a conductive Zn plate by EPD. Cyclic voltammetry data indicate that the coating of Zn with MWCNTs does not affect the Zn oxidation and reduction potential. Oxidation of Zn eventually leads to formation of a ZnO film, protecting the Zn surface from corrosion. When the protective ZnO film is dissolved, the underlying Zn is oxidized, leading to unfavorable loss of Zn. The presence of MWCNTs reduces oxidation during the cathodic sweep, implying that the MWCNT coating partially protects the underlying Zn surface from oxidation during charging. In addition, the MWCNT coated electrodes also facilitate hydrogen formation and show less oxygen limitation reaction, and could thus be envisaged as possible electrode materials for energy storage devices such as bifunctional electrodes for electrolyzers or air cathodes for batteries or fuel cells.
Identification of a Clinically Relevant Signature for Early Progression in KRAS-Driven Lung Adenocarcinoma (2019)

Inducible genetically defined mouse models of cancer uniquely facilitate the investigation of early events in cancer progression, however there are valid concerns about the ability of such models to faithfully recapitulate human disease.&amp;nbsp; We developed an inducible mouse model of progressive lung adenocarcinoma (LuAd) that combines sporadic activation of oncogenic KRasG12D with modest overexpression of c-MYC (KM model). Histological examination revealed a highly reproducible transition from adenoma to locally invasive adenocarcinoma within 6 weeks of oncogene activation.&amp;nbsp; Laser-capture microdissection coupled with RNA-SEQ was employed to determine transcriptional changes associated with tumour progression.&amp;nbsp; Upregulated genes were triaged for relevance to human LuAd using datasets from Oncomine and cBioportal.&amp;nbsp; Selected genes were validated by RNAi screening in human lung cancer cell lines and examined for association with lung cancer patient overall survival using KMplot.com.&amp;nbsp; Depletion of progression-associated genes resulted in pronounced viability and/or cell migration defects in human lung cancer cells.&amp;nbsp; Progression-associated genes moreover exhibited strong associations with overall survival, specifically in human lung adenocarcinoma, but not in squamous cell carcinoma. The KM mouse model faithfully recapitulates key molecular events in human lung cancer and is a useful tool for mechanistic interrogation of LuAd progression.
ChemInform Abstract: Generation and Reaction of Copper(I) Hydride in the Copper(I) Chloride—Tributyltin Hydride—NMP System: Synthesis of 3‐Norcephalosporin. (2000)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Gene-rich UV sex chromosomes harbor conserved regulators of sexual development (2021)

Nonrecombining sex chromosomes, like the mammalian Y, often lose genes and accumulate transposable elements, a process termed degeneration. The correlation between suppressed recombination and degeneration is clear in animal XY systems, but the absence of recombination is confounded with other asymmetries between the X and Y. In contrast, UV sex chromosomes, like those found in bryophytes, experience symmetrical population genetic conditions. Here, we generate nearly gapless female and male chromosome-scale reference genomes of the moss <i>Ceratodon purpureus</i> to test for degeneration in the bryophyte UV sex chromosomes. We show that the moss sex chromosomes evolved over 300 million years ago and expanded via two chromosomal fusions. Although the sex chromosomes exhibit weaker purifying selection than autosomes, we find that suppressed recombination alone is insufficient to drive degeneration. Instead, the U and V sex chromosomes harbor thousands of broadly expressed genes, including numerous key regulators of sexual development across land plants.
Graceful Restart Mechanism for BGP (2007)

community, and requests discussion and suggestions for improvements.
Appearance of the Bona Fide Spiral Tubule of Orf Virus Is Dependent on an Intact 10-Kilodalton Viral Protein (2004)

Parapoxviruses can be morphologically distinguished from other poxviruses in conventional negative staining electron microscopy (EM) by their ovoid appearance and the spiral tubule surrounding the virion's surface. However, this technique may introduce artifacts. We have examined Orf virus (ORFV; the prototype species of the Parapoxvirus genus) by cryoelectron microscopy (cryo-EM) and cryo-negative staining EM. From these studies we suggest that the shape and unique spiral tubule are authentic features of the parapoxviruses. We also constructed an ORFV mutant deleted of a gene encoding a 10-kDa protein, which is an orthologue of the vaccinia virus (VACV) 14-kDa fusion protein, and investigated its ultrastructure. This mutant virus multiplied slowly in permissive cells and produced infectious but morphologically aberrant particles. Mutant virions lacked the spiral tubule but displayed short disorganized tubules similar to those observed on the surface of VACV. In addition, thin extensions or loop-like structures were appended to the ORFV mutant particles. We suggest that these appended structures arise from a failure of the mutant virus particles to properly seal and that the sealing activity is dependent on the 10-kDa protein.
Use of a Boundary-Fitted Coordinate Transformation for Unsteady Heat Conduction Problems in Multiconnected Regions With Arbitrarily Shaped Boundaries (1985)

Technical Briefs Use of a Boundary-Fitted Coordinate Transformation for Unsteady Heat Conduction Problems in Multiconnected Regions With Arbitrarily Shaped Boundaries S. Uchikawa, S. Uchikawa Energy Research Laboratory, Hitachi Ltd., Ibaragi 316, Japan Search for other works by this author on: This Site PubMed Google Scholar R. Takeda R. Takeda Energy Research Laboratory, Hitachi Ltd., Ibaragi 316, Japan Search for other works by this author on: This Site PubMed Google Scholar Author and Article Information S. Uchikawa Energy Research Laboratory, Hitachi Ltd., Ibaragi 316, Japan R. Takeda Energy Research Laboratory, Hitachi Ltd., Ibaragi 316, Japan J. Heat Transfer. May 1985, 107(2): 494-498 (5 pages) https://doi.org/10.1115/1.3247448 Published Online: May 1, 1985 Article history Online: October 20, 2009
Cracks in the wall: Entrepreneurial action theory and the weakening presumption of intended rationality (2022)

Entrepreneurship scholarship finds itself in something of a quandary concerning rationality. While an increasingly large body of empirical work has found evidence of less-deliberative and even impulsive drivers of business venturing, the dominant theories of entrepreneurial action remain anchored to the assumption that intended rationality is a defining attribute of entrepreneurship. The growing schism between entrepreneurial action theory (EAT) on the one hand, and empirics and practice on the other hand, represents a consequential and exciting opportunity for the field to revisit its core assumptions regarding rationality, particularly the presence, role, and function of rational intentionality. In this study, we undertake a review and exploratory investigation of the assertion that without reasoned intentionality there is no entrepreneurship. Our work generates three important insights that contribute to rethinking key facets of the most prominent and influential EATs: alternative, non-rational pathways to business venturing exist with a non-ignorable prevalence; a proclivity towards reasoned intentionality is not invariably prescriptive; and, less-reasoned, less-deliberative tendencies do not constitute an entrepreneurial death sentence. Rather, entrepreneurs (including highly successful ones) embody a shifting blend of rational and non-rational proclivities, motivations, decisions, and actions.
In-Cell NMR Spectroscopy (2001)

The recent development of "in-cell NMR" techniques by two independent groups has demonstrated that NMR spectroscopy can be used to characterize the conformation and dynamics of biological macromolecules inside living cells. In this article, we describe different methods and discuss current and future applications as well as critical parameters of this new technique. We show experimental results, compare them with traditional in vitro experiments, and demonstrate that differences between the in vitro and the in vivo state of a macromolecule exist and can be detected and characterized.