gebhart/patrepeval huggingface dataset:
Proapoptotic Bim regulates antigen-specific NK cell contraction and the generation of the memory NK cell pool after cytomegalovirus infection (2014)

Apoptosis is critical for the elimination of activated lymphocytes after viral infection. Proapoptotic factor Bim (Bcl2l11) controls T lymphocyte contraction and the formation of memory T cells after infection. Natural killer (NK) cells also undergo antigen-driven expansion to become long-lived memory cells after mouse cytomegalovirus (MCMV) infection; therefore, we examined the role of Bim in regulating the MCMV-driven memory NK cell pool. Despite responding similarly early after infection, Bcl2l11(-/-) Ly49H(+) NK cells show impaired contraction and significantly outnumber wild-type (WT) cells after the expansion phase. The inability to reduce the effector pool leads to a larger Bcl2l11(-/-) NK memory subset, which displays a less mature phenotype (CD11b(lo), CD27(+)) and lower levels of NK cell memory-associated markers KLRG1 and Ly6C. Bcl2l11(-/-) memory NK cells demonstrate a reduced response to m157-mediated stimulation and do not protect as effectively as WT memory NK cells in an MCMV challenge model. Thus, Bim-mediated apoptosis drives selective contraction of effector NK cells to generate a pool of mature, MCMV-specific memory cells.
Enhanced Intramolecular Charge Transfer in New Type Donor–Acceptor Substituted Perylenes (2012)

Here we report the facile synthesis and physical characterization of new type N-(2,6-diisopropylphenyl)-3,4-perylenedicarboximides (PMI) with alkyl-substituted quinoline-4(1H)-ylidenemethyl or acridine-9(10H)-ylidenemethyl units as strong donors in the 9-position. When compared to parent PMI, these perylene dyes, 9-((1-methylquinoline-4(1H)-ylidene)methyl)-PMI, 9-((1-benzylquinoline-4(1H)ylidene)methyl)-PMI, 9-((1-heptylquinoline-4(1H)-ylidene)methyl)-PMI, and 9-((10-methylacridine-9(10H)-ylidene)methyl)-PMI, show a pronounced bathochromic shift of their electronic absorption with solvatochromism because of their intramolecular charge transfer. The solvatochromic behavior of these dyes is further confirmed by second-order nonlinear optical experiments. Remarkably high second-order nonlinear optical values (βHRS up to 1300 ± 50 × 10–30 esu at 880 nm in dichloromethane) are obtained by femtosecond hyper-Rayleigh scattering. The one-step synthesis together with the spectroscopic, solvatochromic, and nonlinear optical characteristics qualify these new types of perylene dyes as promising candidates for solvent polarity probes, photovoltaics, or nonlinear optical applications.
56.5L: <i>Late‐News Paper</i>: All‐Printed Oxide Thin Film Transistor Arrays for High Resolution Active Matrix (2013)

Abstract We have demonstrated all‐printed oxide TFT arrays for 100 ppi RGB active matrix fabricated by inkjet printing and spin coating without photolithography and mask process. The average mobility of TFTs was 7.49 ± 1.67 cm 2 /Vs on a thermally oxidized SiO 2 /Si substrate. Moreover, in the case of using of a printed oxide high‐k gate insulator and an n‐type doped active layer on a glass substrate, the average mobility of all‐printed oxide TFTs was 2.54 ± 0.32 cm 2 /Vs.
<sup>1</sup>H and <sup>13</sup>C chemical shifts for bis(benzopyridinium) dibromides with semirigid aromatic linkers (2002)

Abstract The 1 H and 13 C NMR resonances for four bisquinolinium and four bisisoquinolinium dibromides were completely and unequivocally assigned, using the concerted application of one‐ and two‐dimensional NMR techniques including nuclear Overhauser effect difference, DEPT, COSY, HETCOR and long‐range 13 C– 1 H correlation spectroscopy. Atomic charges of the heterocyclic moieties of model cations such as 1‐methylquinolinium and 2‐methylisoquinolinium are shown to be linearly related to the 13 C chemical shifts of the cationic heads of the title compounds. Copyright © 2002 John Wiley &amp; Sons, Ltd.
Engineering Hydrogel Adhesion for Biomedical Applications via Chemical Design of the Junction (2021)

Hydrogel adhesion inherently relies on engineering the contact surface at soft and hydrated interfaces. Upon contact, adhesion normally occurs through the formation of chemical or physical interactions between the disparate surfaces. The ability to form these adhesion junctions is challenging for hydrogels as the interfaces are wet and deformable and often contain low densities of functional groups. In this Review, we link the design of the binding chemistries or adhesion junctions, whether covalent, dynamic covalent, supramolecular, or physical, to the emergent adhesive properties of soft and hydrated interfaces. Wet adhesion is useful for bonding to or between tissues and implants for a range of biomedical applications. We highlight several recent and emerging adhesive hydrogels for use in biomedicine in the context of efficient junction design. The main focus is on engineering hydrogel adhesion through molecular design of the junctions to tailor the adhesion strength, reversibility, stability, and response to environmental stimuli.
New Frontiers of Sound: A Quiet Revolution (2020)

Sound waves are all around us. They govern vibrations from the sweetest of sound to the most destructive earthquakes. Now, a team of researchers across the US has embarked on an ambitious project, named ‘New Frontiers of Sound’, which will pave the way for advanced technologies that operate using sound waves. Combining the expertise of more than 30 leading scientists and engineers from across the field of acoustics, the project promises to discover new properties of sound, while offering exciting opportunities for researchers from a diverse range of backgrounds.
Human mesenchymal stem cells are resistant to UV-B irradiation (2019)

Albeit being an effective therapy for various cutaneous conditions, UV-B irradiation can cause severe skin damage. While multipotent mesenchymal stem cells (MSCs) may aid the regeneration of UV-B-induced skin injuries, the influence of UV-B irradiation on MSCs remains widely unknown. Here, we show that human MSCs are relatively resistant to UV-B irradiation compared to dermal fibroblasts. MSCs exhibited higher clonogenic survival, proliferative activity and viability than dermal fibroblasts after exposure to UV-B irradiation. Cellular adhesion, morphology and expression of characteristic surface marker patterns remained largely unaffected in UV-irradiated MSCs. The differentiation ability along the adipogenic, osteogenic and chondrogenic lineages was preserved after UV-B treatment. However, UV-B radiation resulted in a reduced ability of MSCs and dermal fibroblasts to migrate. MSCs exhibited low apoptosis rates after UV-B irradiation and repaired UV-B-induced cyclobutane pyrimidine dimers more efficiently than dermal fibroblasts. UV-B irradiation led to prolonged p53 protein stability and increased p21 protein expression resulting in a prolonged G2 arrest and senescence induction in MSCs. The observed resistance may contribute to the ability of these multipotent cells to aid the regeneration of UV-B-induced skin injuries.
Progressive multifocal leukoencephalopathy lesion and brain parenchymal segmentation from MRI using serial deep convolutional neural networks (2020)

Progressive multifocal leukoencephalopathy (PML) is a rare opportunistic brain infection caused by the JC virus and associated with substantial morbidity and mortality. Accurate MRI assessment of PML lesion burden and brain parenchymal atrophy is of decisive value in monitoring the disease course and response to therapy. However, there are currently no validated automatic methods for quantification of PML lesion burden or associated parenchymal volume loss. Furthermore, manual brain or lesion delineations can be tedious, require the use of valuable time resources by radiologists or trained experts, and are often subjective. In this work, we introduce JCnet (named after the causative viral agent), an end-to-end, fully automated method for brain parenchymal and lesion segmentation in PML using consecutive 3D patch-based convolutional neural networks. The network architecture consists of multi-view feature pyramid networks with hierarchical residual learning blocks containing embedded batch normalization and nonlinear activation functions. The feature maps across the bottom-up and top-down pathways of the feature pyramids are merged, and an output probability membership generated through convolutional pathways, thus rendering the method fully convolutional. Our results show that this approach outperforms and improves longitudinal consistency compared to conventional, state-of-the-art methods of healthy brain and multiple sclerosis lesion segmentation, utilized here as comparators given the lack of available methods validated for use in PML. The ability to produce robust and accurate automated measures of brain atrophy and lesion segmentation in PML is not only valuable clinically but holds promise toward including standardized quantitative MRI measures in clinical trials of targeted therapies. Code is available at: https://github.com/omarallouz/JCnet.
HIGH STRAIN AND BIOCOMPATIBLE SCREEN PRINTED NANOCOMPOSITE BASED CONDUCTIVE PDMS STRAIN SENSORS (2012)

Strain sensors capable of operating in high strain conditions remain a technical challenge. In particular, biocompatible strain sensor technology is needed that satisfies the following requirements: (1) construction from low modulus materials that approach values of soft biological tissues and (2) high strain operation ( 20%). We present a screen printed, nanocomposite-based conductive polydimethylsiloxane (CPDMS) strain sensor capable of 40% strain operation with a gauge factor (GF) >100. Strain sensing using CPDMS sensors containing multi-walled carbon nanotubes (MWNT), graphene nanoplatelets (GNP), or a mixture of both nanocarbon filler materials was demonstrated. The combination of high strain operation, high GF, and biocompatible construction pave the way for minimally invasive in vivo strain measurements. Strain sensors were characterized according to their conductivity, zero current resistance (ZCR), thermal coefficients of resistance (TCR), and gauge factor.
Molecular Methods in Biological Systems (2004)

libraries were constructed. The microbial diversity in each clone library was assessed by amplified ribosomal DNA restriction analysis (ARDRA) The complete 64508 bp nucleotide sequence of the IncP-1beta antibiotic-resistance plasmid pB10, which was isolated from a wastewater treatment plant in Germany and mediates resistance against the antimicrobial agents amoxicillin, streptomycin, sulfonamides, and tetracycline and against mercury ions, was determined and analyzed. Experiments with genetic material from this plasmid suggests that recombination in the environment is common, which may enhance plasmid diversity and bacterial adaptability
Synthesis, Crystal Structures and Magnetic Properties of a Phenoxo‐Bridged Dinuclear Cu<sup>II</sup> Complex and a Dicyanamide Bridged Novel Molecular Rectangle Based on It (2011)

Abstract The phenoxo‐bridged dinuclear Cu II complex [Cu 2 L 2 (NCNCN) 2 ] ( 1 ) and the dicyanamide‐bridged molecular rectangle [Cu 4 L 4 {μ 1,5 ‐(NCNCN) 2 }] · (ClO 4 ) 2 (H 2 O) 2 ( 2 ) were synthesized using the tridentate reduced Schiff‐base ligand HL {2‐[(2‐dimethylamino‐ethylamino)methyl]phenol}. The complexes were characterized by X‐ray structural analyses and variable‐temperature magnetic susceptibility measurements. Complex 2 was formed through the joining of the phenoxo‐bridged dinuclear Cu 2 O 2 cores of 1 via the μ 1,5 ‐bridging mode of dicyanamide. The structural properties of the Cu 2 O 2 cores in two complexes are significantly different. The geometry of the copper ions is distorted trigonal bipyramid in 1 but is nearly square‐pyramidal in 2 . These differences have a marked effect on the magnetic properties of two compounds. Although both are antiferromagnetically coupled, the coupling constants ( J = –185.2 and –500.9 cm –1 for 1 and 2 , respectively) differ considerably.
Interleukin-1β inhibitors for the management of acute gout flares: a systematic literature review (2023)

PROSPERO ID: CRD42021267670.
Aging and skill acquisition: Learning-performance distinctions. (1994)

This research examined the hypothesis that a conservative response bias in older persons interferes with the acquisition and mastery of cognitive skill. Twenty younger and 20 older Ss performed both consistent mapping and varied mapping versions of a memory search task. Half of the Ss in each group performed under speed stress instructions, whereas the remaining Ss performed under accuracy stress instructions. Older Ss exhibited less skilled performance than did younger Ss. A power function analysis attributed this to both age-related differences in the rate of associative learning and differences in asymptotic levels of performance. These results are at odds with A. D. Fisk and W. A. Roger's (1991) hypothesis that age-related differences in automatization do not occur in memory search tasks. Results are interpreted in terms of a learning vs. performance distinction.
A Toolkit to Quantify Target Compounds in Thin Layer Chromatography Experiments (2018)

Thin layer chromatography (TLC) is one of the basic experimental procedures in chemistry and allows the demonstration of various chemical principles in an educational setting. An often-overlooked aspect of TLC is the capability to quantify isolated target compounds in an unknown sample. Here, we present a suitable route to implement quantitative analysis in a lesson plan. We provide a free, stand-alone software that allows students to obtain quantitative information and present two suitable experiments, namely the absorbance-based quantification of the colorant Sudan IV and the fluorescence-based quantification of Rhodamine 6G, a fluorophore widely used in biotechnology. Students conduct TLC experiments following established routes, then take pictures of their TLC plates with their mobile phones and finally quantify the amount of different compounds in the separate bands they observe.
Weight loss and metabolic changes of morbidly obese patients after gastric partitioning operation (1985)

Gastric partitioning operation resulted in an initial rapid body weight loss over 3 months with a sustained reduction over 1 year; there was a metabolic utilization shift to fat with carbohydrate and protein sparing; no metabolic parameter was predictive of weight loss; and temporally, the rapid weight loss was paralleled by a significant metabolic utilization shift, and the sustained loss was paralleled by a stabilization of this shift.
A New Approach to Steady-State Modeling, Analysis, and Design of Power Converters (2021)

Steady-state models of power converters that provide accurate closed-form expressions for converter waveforms are extremely valuable for converter analysis and design, and enable comparative evaluation of different converter topologies. An obstacle in the development of such models is the inherent nonlinearity of switching power converters. This article presents a systematic procedure to model a broad class of power converters using ordinary differential equations (ODEs) with periodic and discontinuous inputs, and provides an approach to determine closed-form expressions for their steady-state waveforms. The formal mathematical proof of the proposed approach to finding closed-form expressions for the steady-state solution of ODEs with periodic and discontinuous inputs, Laplace-based theorem (LBT), is also presented. The presented modeling procedure and LBT, collectively called Laplace-based steady-state modeling (LBSM), serves as an effective analysis and design tool for power converters. The value of LBSM is demonstrated by using it to obtain closed-form expressions for the steady-state waveforms of different types of converters. In particular, two commonly used topologies—the series resonant converter and the phase-shift converter—are analyzed and compared using LBSM and their optimum operating conditions and applications are discussed. The converter waveforms, soft switching ranges and other characteristics obtained using LBSM are also validated through simulations and experiments.
Identificación de especies de Leishmania en pacientes y flebotominos en áreas de transmisión en una región de Perú (2011)

Objectives. To identify the species of Leishmania present in the skin lesions of patients and Lutzomyias living in endemic areas of La Libertad, Peru. Materials and methods. Molecular methods based on PCR and RFLP were used, which allowed to have efficient data with small amounts of samples (small specimens), due to their high sensitivity and ease of application in the field work. Results. The results of PCR of clinical samples of patients and insect vectors showed the presence of Leishmania (V.) peruviana as a major causative agent of andean leishmaniasis transmitted by Lutzomyia peruensis. The presence of Leishmania (V.) guyanensis in Lutzomyia ayacuchensis, was found as well. Conclusions. The presence of L. (V.) peruviana and L. (V.) guyanensis in the Andean areas under study was found. These findings remark the need of a wider research about the geographical distribution of L. (V.) guyanensis and clinical features related to the infection in endemic areas of cutaneous leishmaniasis.
Formulation development of the biocontrol agent Bacillus subtilis strain CPA-8 by spray-drying (2012)

New shelf-stable and effective formulations of a biocontrol agent have been obtained by spray-drying to control brown rot on peach.
Policy-Aware Provisioning of Cloud Applications (2013)

agement Planlets that allow the implementation of policy- aware provisioning logic in a reusable way independently from individual applications. The presented framework enables Cloud providers as well as application developers to specify security requirements for the provisioning without the need to have the deep technical management knowledge needed in other approaches. In addition, the concept allows security experts of different domains to work together in a collaborative way. We evaluate the approach in terms of performance, complexity, economics, feasibility, extensibility, and the implementation of a prototype. The remainder of this paper is structured as follows. In Section II, we explain fundamentals and motivate our approach by a scenario presented in Section III. In Section IV, we introduce Provisioning Policies. Section V describes the Management Framework that is extended by our approach, which is presented in Section VI. Section VII evaluates the approach and Section VIII reviews related work. We conclude this paper and give an outlook on future work in Section IX.
Progress of separation of CO_2 from flue gas by membrane absorption (2008)

As a new technology of carbon dioxide separation from flue gas,membrane absorption has caught more attention.An introduction of the fundamentals of membrane absorption is presented,together with an analysis of membrane absorption from three aspects,including system process,absorption solution,and membrane contactors and membrane materials.In conclusion,the prospect of future development is discussed.
A Review on Energy Efficient Approaches for Cloud Computing (2023)

Cloud computing is becoming widely adopted, which increases both its user’s efficiency and the provider’s profitability. Virtualization is assisting the cloud in increasing resource utilization, but it is also increasing operational expenses due to increased energy usage. This paper studies and sheds light on the contemporary modern day research on cloud energy management techniques such as ballooning, virtual machine migration, resource allocation (RA), EEEHVMC (Energy Efficiency Heuristic with Virtual Machine Consolidation), three-way decision (VMM3WD), and others that can be implemented in fundamental software level, virtualization and various applications and the operating system itself. It studies various measures to reduce the energy usage and clearly contributes to minimizing existing environmental problems and curbing it. This paper clearly explains each approach, including its benefits, drawbacks, and future prospects, taking into account modern day parameters. This article also shed light on the burdensome parameters and criteria’s that cloud organizations and cloud data centers must follow.
Energetics of sodium transport in toad urinary bladder. (1978)

The ratio of the rate of transepithelial sodium transport, JNa, across the isolated toad urinary bladder to the simultaneously measured rate of transport-dependent metabolism, JsbCO2, has been measured as a function of the transepithelial electrical voltage, deltapsi. The ratio remains constant with a mean value of 18 to 20 over the range of imposed voltages of 0 to +70 mV. With increasing hyperpolarization of the bladder, JNa decreases and the calculated electromotive force or apparent "ENa" of the sodium pump increases. From thermodynamic and kinetic arguments it is shown that the apparent "ENa" approaches the maximal electrochemical potential gradient, ENa, against which sodium can be transported by this tissue only when JNa approximately 0. At this unique condition F ENa (in which F is the Faraday constant) is the maximal free energy of the chemical reaction driving sodium transport and thus equal to the maximal extramitochondrial phosphorylation potential and the maximal free energy of the mitochondrial respiratory chain within the transporting cells.
CRISPR-Cas Gene Editing to Cure Serious Diseases: Treat the Patient, Not the Germ Line (2015)

CRISPR-Cas9 is a powerful new technique that allows for precise editing of DNA, the genetic code in all the cells of our body. It is also broadly available and easy to use, which has raised the fea...
Malignant fibrous histiocytoma: Inherited and sporadic forms have loss of heterozygosity at chromosome bands 9p21–22—evidence for a common genetic defect (2000)

Hereditary cancers represent a unique opportunity to investigate the genetic etiology of their more common sporadic forms. We recently established genetic linkage for the rare autosomal-dominant bone dysplasia/cancer syndrome, diaphyseal medullary stenosis with malignant fibrous histiocytoma (DMS-MFH), to a 3-cM region on chromosome bands 9p21–22. This hereditary cancer syndrome is characterized by bone infarctions, cortical growth abnormalities, pathologic fractures, and painful debilitation. Most notably, 35% of affected individuals develop bone MFH, a sarcoma that, in its sporadic form, accounts for 6% of all bone cancers. To determine whether the hereditary and sporadic forms of bone MFH are genetically linked, we performed loss of heterozygosity (LOH) studies of the DMS-MFH critical region. In addition to the hereditary specimen, 71% (5/7) of informative sporadic bone MFH specimens displayed LOH for markers within that same region. Definition of the minimal region of LOH overlap effectively limited the DMS-MFH gene to a 2-cM region between markers D9S736 and D9S171. In summary, these studies suggest that a common genetic etiology underlies the autosomal-dominant and sporadic forms of this sarcoma and provide the basis for identifying the putative MFH tumor suppressor gene. Genes Chromosomes Cancer 27:191–195, 2000. © 2000 Wiley-Liss, Inc.
Metabolomics relative quantitation with mass spectrometry using chemical derivatization and isotope labeling (2008)

Comprehensive detection and quantitation of metabolites from a biological source constitute the major challenges of current metabolomics research. Two chemical derivatization methodologies, butylation and amination, were applied to human serum for ionization enhancement of a broad spectrum of metabolite classes, including steroids and amino acids. LC-ESI-MS analysis of the derivatized serum samples provided a significant signal elevation across the total ion chromatogram to over a 100-fold increase in ionization efficiency. It was also demonstrated that derivatization combined with isotopically labeled reagents facilitated the relative quantitation of derivatized metabolites from individual as well as pooled samples.