gebhart/patrepeval huggingface dataset:
LRRC45 contributes to early steps of axoneme extension (2018)

Cilia perform essential signalling functions during development and tissue homeostasis. A key event in ciliogenesis occurs when the distal appendages of the mother centriole form a platform that docks ciliary vesicles and removes CP110-Cep97 inhibitory complexes. Here, we analysed the role of LRRC45 in appendage formation and ciliogenesis. We show that the core appendage proteins Cep83 and SCLT1 recruit LRRC45 to the mother centriole. Once there, LRRC45 recruits the keratin-binding protein FBF1. The association of LRRC45 with the basal body of primary and motile cilia in both differentiated and stem cells reveals a broad function in ciliogenesis. In contrast to the appendage components Cep164 and Cep123, LRRC45 was not essential for either docking of early ciliary vesicles or for removal of CP110. Rather, LRRC45 promotes cilia biogenesis in CP110-uncapped centrioles by organising centriolar satellites, establishing the transition zone and promoting the docking of Rab8 GTPase-positive vesicles. We propose that, instead of acting solely as a platform to recruit early vesicles, centriole appendages form discrete scaffolds of cooperating proteins that execute specific functions that promote the initial steps of ciliogenesis.
Early age–related atrophy of cutaneous lymph nodes precipitates an early functional decline in skin immunity in mice with aging (2022)

Secondary lymphoid organs (SLOs) (including the spleen and lymph nodes [LNs]) are critical both for the maintenance of naive T (TN) lymphocytes and for the initiation and coordination of immune responses. How they age, including the exact timing, extent, physiological relevance, and the nature of age-related changes, remains incompletely understood. We used “time stamping” to indelibly mark newly generated naive T cells (also known as recent thymic emigrants) (RTEs) in mice, and followed their presence, phenotype, and retention in SLOs. We found that SLOs involute asynchronously. Skin-draining LNs atrophied by 6 to 9 mo in life, whereas deeper tissue-draining LNs atrophied by 18 to 20 mo, as measured by the loss of both TN numbers and the fibroblastic reticular cell (FRC) network. Time-stamped RTEs at all ages entered SLOs and successfully completed postthymic differentiation, but the capacity of older SLOs to maintain TN numbers was reduced with aging, and that trait did not depend on the age of TNs. However, in SLOs of older mice, these cells exhibited an emigration phenotype (CCR7loS1P1hi), which correlated with an increase of the cells of the same phenotype in the blood. Finally, upon intradermal immunization, RTEs generated in mice barely participated in de novo immune responses and failed to produce well-armed effector cells detectable in blood as early as by 7 to 8 mo of age. These results highlight changes in structure and function of superficial secondary lymphoid organs in laboratory mice that are earlier than expected and are consistent with the long-appreciated reduction of cutaneous immunity with aging.
Seroepidemiology of HTLV-III antibodies in a remote population of eastern Zaire. (1985)

A human retrovirus--human T cell lymphotropic virus-III (HTLV-III)--has recently emerged as the probable cause of acquired immunodeficiency syndrome (AIDS). In May 1984, 250 outpatients at a hospital in a remote area of eastern Zaire were surveyed for AIDS type illnesses and the prevalence of antibodies against HTLV-III determined by an enzyme linked immunosorbent assay using disrupted whole HTLV-III virus as the antigen. No clinical cases of AIDS were diagnosed among these patients. Overall, 31 (12.4%) had clearly positive ratios (greater than or equal to 5.0) and a further 30 (12.0%) had borderline ratios (3.0- less than 5.0). Western blots of serum samples from subjects with antibodies yielded bands consistent with HTLV-III as found in American patients with AIDS and members of groups at risk of AIDS. The prevalence of antibody was highest in childhood (p = 0.02); among adults prevalence rose slightly with age. HTLV-III antibodies were more common among the uneducated (p = 0.006), agricultural workers (p = 0.03), and rural residents (p = 0.006), but the Western blot bands were generally weak in this group. By contrast, one urban resident had strong bands. The relatively high prevalence of antibodies among the rural poor in this area of Zaire suggests that HTLV-III or a closely related, cross reactive virus may be endemic in the region. A different natural history of infection, perhaps in childhood, may also explain the findings.
Sequential Single-Cell Transcriptional and Protein Marker Profiling Reveals TIGIT as a Marker of CD19 CAR-T Cell Dysfunction in Patients with Non-Hodgkin Lymphoma (2022)

This is the first study investigating the mechanisms linked to CAR-T patient responses based on the sequential analysis of manufactured and infused CAR-T cells using single-cell RNA and protein expression data. Furthermore, our findings are the first to demonstrate an improvement of CAR-T cell efficacy with TIGIT inhibition alone. This article is highlighted in the In This Issue feature, p. 1825.
Preclinical Safety Evaluation of Inhaled Cyclosporine in Propylene Glycol (2007)

Cyclosporine inhalation solution has the potential to improve outcomes following lung transplantation by delivering high concentrations of an immunosuppressant directly to the allograft while minimizing systemic drug exposure and associated toxicity. The objective of these studies was to evaluate the potential toxicity of aerosolized cyclosporine formulated in propylene glycol when given by inhalation route to rats and dogs for 28 days. Sprague-Dawley rats received total inhaled doses of 0 (air), 0 (vehicle, propylene glycol), 7.4, 24.3, and 53.9 mg cyclosporine/kg/day. In a separate study, beagle dogs were exposed to 0, 4.4, 7.7, and 9.7 mg cyclosporine/kg/day. Endpoints used to evaluate potential toxicity of inhaled cyclosporine were clinical observations, body weight, food consumption, respiratory functions, toxicokinetics, and clinical/anatomic pathology. Daily administration of aerosolized cyclosporine did not result in observable accumulation of cyclosporine in blood or lung tissue. Toxicokinetic analysis from the rat study showed that the exposure of cyclosporine was approximately 18 times higher in the lung tissue compared to the blood. Systemic effects were consistent with those known for cyclosporine. There was no unexpected systemic toxicity or clinically limiting local respiratory toxicity associated with inhalation exposure to cyclosporine inhalation solution at exposures up to 2.7 times the maximum human exposure in either rats or dogs. There were no respiratory or systemic effects of high doses of propylene glycol relative to air controls. These preclinical studies demonstrate the safety of aerosolized cyclosporine in propylene glycol and support its continued clinical investigation in patients undergoing allogeneic lung transplantation.
Transfer of fluid by Peristalsis of Side Wall in Open Channel (2016)

Peristaltic pumps can transfer fluid without complicated actuating mechanism. They are studied especially in the MEMS field because of the compactness and simplicity of its structure. In general, the wall of the pump vibrates such as sine function. This study investigates the mechanism of fluid transfer of the peristaltic wall in the view point of wave propagation by using Cell automata(CA). The vibrating amplitude of peristaltic wall cannot be neglected, so reflecting wave occurs when the wave propagates through the peristaltic wall. Long wave of water in an open channel is used in the experiments. To examine the validity of this method, CA simulation results and experimental results are compared.
Amplified Responsiveness of Multilayered Polymer Grafts: Synergy between Brushes and Hydrogels (2015)

The responsive properties of surface-grafted polymer films in aqueous media can be amplified by covalently layering thermosensitive brushes and hydrogels. This was demonstrated by synthesizing layers of linear poly(N-isopropylacrylamide) (PNIPAM) brushes, alternating with cross-linked, poly(hydroxyethyl)methacrylate (PHEMA) hydrogels via sequential surface-initiated atom-transfer radical polymerization (SI-ATRP) steps. Below the lower critical solution temperature (LCST) of PNIPAM, brush/hydrogel multilayered films swell similarly to linear PNIPAM homopolymer brushes, as measured by liquid ellipsometry. In contrast, above the LCST, the PHEMA hydrogel interlayer acts as stiffening element within the collapsed multilayered film, as monitored by atomic force microscopy (AFM) nanoindentation and lateral force microscopy (LFM). This translates into a 10-fold increase in Young’s modulus by the collapsed, layered films compared to PNIPAM homopolymer analogues. The (macro)molecular continuity between the brush main chains and hydrogel constituents thus enables a chemically robust layering to form graded, quasi-3D grafted polymer architectures, which display a concerted and amplified temperature-triggered transition.
Watt-power scalability of the near-infrared and UV, PPKTP and PPLT based sources using 40 dBm seeded erbium fibre amplifier (2001)

Summary form only given. We report on increased power scalability for generation of 772 nm and 386 nm radiation using second harmonic (SH) generation in periodically poled KTP (PPKTP) and LiTaO/sub 3/ (PPLT). We have demonstrated the power-up scalability of 772 nm PPKTP based source to 3.5 W average power level, and of 386 nm PPLT based source to 235 mW level using 40 dBm seeded erbium fibre amplifier. The sources benefit from high stability, compact format, fibre-based design and high wall-plug efficiency.
N‐NITRO‐L‐ARGININE AND INDOMETHACIN DO NOT AFFECT ENDOTHELIN‐INDUCED CONSTRICTION OF LARGE AND SMALL CORONARY ARTERIES IN THE ANAESTHETIZED GREYHOUND (1996)

1. The aim of this study was to investigate whether endothelin-1 (ET-1)-induced constriction of large and small coronary arteries in the anaesthetized greyhound is modulated by the endogenous release of nitric oxide or prostanoids. 2. ET-1 (1-100 ng/kg) and the alpha1-adrenoceptor agonist phenylephrine (0.5-2 mu g/kg), when injected directly into the circumflex coronary artery, caused dose-dependent decreases in epicardial coronary artery diameter and coronary vascular conductance without affecting systemic arterial pressure or the rate and force of cardiac contraction. 3. Inhibition of NO synthesis with N-nitro-L-arginine (NOLA, 5 mg/kg, i.c.) decreased coronary artery diameter, coronary conductance and heart rate and increased arterial pressure. The coronary vasoconstrictor response to ET-1 was unaffected by NOLA. By contrast, NOLA significantly increased the phenylephrine-induced constriction of the epicardial coronary artery but not the resistance vessels. 4. Indomethacin (5 mg/kg, i.v.), an inhibitor of cyclo-oxygenase, significantly decreased epicardial coronary artery diameter but did not affect coronary conductance. Indomethacin had no effect on the coronary vascular responses to ET-1 or phenylephrine. Combined treatment with NOLA plus indomethacin also failed to affect the coronary vasoconstrictor effects of ET-1. 5. Basal release of NO and vasodilator prostanoids modulated resting coronary vascular tone but did not influence the vasoconstrictor responses to endothelin in either large or small coronary arteries.
Interaction of carboxymethylchitin with the Langmuir monolayers of cationic surfactants (1997)

The monolayer technique has been used to study the interfacial behaviour of complexes formed between cationic surfactants (insoluble in water: dioctadecyldimethylammonium bromide, DODAB) and anionic polyelectrolyte (carboxymethylchitin, CMCh).
Process integration of best in class specific-on resistance of 20V to 60V 0.18&amp;#x00B5;m bipolar CMOS DMOS technology (2013)

The evolution of Bipolar CMOS DMOS (BCD) technology leads in the improvement of design technology to produce better device performance with fabrication cost effectiveness as concerned. In this paper, 0.18μm BCD technology with linear p-top layer N-channel LDMOS structure from 20V to 60V is presented with best in class Ron for both N-channel and P-channel LDMOS compared to recent BCD technology works. The optimization of current gain of 20V BJT has been done by adjustment of emitter width and boron doping concentration of base with increasing of current gain up to ten times higher without significantly change the breakdown voltage.
Bijels stabilized using rod-like particles (2015)

Bicontinuous interfacially jammed emulsion gels, in short 'bijels', rely on a trapped layer of colloidal particles for their stability. These structures have traditionally been created using spherical colloidal particles. Here we show for the first time the use of rod-shape particles to stabilize bijels. We show that domain size decreases more rapidly with particle concentration in the case of rods compared to spheres. Large-scale analysis and detailed examination of images show that the packing fraction of rods is much higher than expected, in part, due to the role of 'flippers'.
Premixed calcium silicate cement for endodontic applications (2011)

Calcium silicate-based materials (also called MTA) are increasingly being used in endodontic applications. However, the handling properties of MTA are not optimal when it comes to injectability and cohesion. Premixing the cements using glycerol avoids these issues. However, there is a lack of data on the effect of common cement variables on important properties of premixed cements for endodontic applications. In this study, the effects of liquid-to-powder ratio, amount of radiopacifier and amount of calcium sulfate (added to control the setting time) were screened using a statistical model. In the second part of the study, the liquid-to-powder ratio was optimized for cements containing three different amounts of radiopacifier. Finally, the effect of using glycerol rather than water was evaluated in terms of radiopacity. The setting time was found to increase with the amount of radiopacifier when the liquid-to-powder ratio was fixed. This was likely due to the higher density of the radiopacifier in comparison to the calcium silicate, which gave a higher liquid-to-powder ratio in terms of volume. Using glycerol rather than water to mix the cements led to a decrease in radiopacity of the cement. In conclusion, we were able to produce premixed calcium silicate cements with acceptable properties for use in endodontic applications.
Combination of Injectable Multiple Growth Factor–Releasing Scaffolds and Cell Therapy as an Advanced Modality to Enhance Tissue Neovascularization (2010)

Vasculogenic progenitor cell therapy is significantly enhanced by in vivo tissue engineering providing a proangiogenic and provasculogenic growth factor-enriched microenvironment. Therefore, combined use of scaffold-released growth factors and cell therapy improves neovascularization in ischemic diseases and may translate into more pronounced clinical effects.
On-Road Real-Time Hyper-Local Air Quality Monitoring Based on Mobile IoT Enabled by a Network of Vehicles Equipped with Air Quality Sensors (2023)

&lt;div class="section abstract"&gt;&lt;div class="htmlview paragraph"&gt;People are exposed to disproportionately high concentrations of air pollutants daily on and near the road during their ride, walk, and residency. Currently air quality monitoring is performed mostly at background locations far away from the road and distinctive point, line, and area emission sources, failing to report concentrations on the road. This study presents an innovative air quality monitoring method using a network of vehicles equipped with air quality sensors (AQSs). Vehicles are connected to internet through either cell link or apps used by drivers. A cloud server collects the data and updates air quality concentration maps every 10 minutes. The study presents very high temporal and spatial resolution maps at a fraction of the cost of previous studies. These high spatiotemporal resolution air quality maps can be used to reduce public exposure in many ways including but not limited to cabin air flap door control, eco-driving route for navigation, and daily exposure monitoring. The method has a potential to be deployed worldwide.&lt;/div&gt;&lt;/div&gt;
Soft and hard tissue modifications around tissue‐level Laser‐Lok implants (2018)

Background Laser-Lok is a promising computer-controlled laser ablation technique which creates an implant neck with precision-engineered cell-sized microchannels (size 8–12 micron). This neck micromorphology may have the potential to achieve create a connective tissue attachment that may prevent apical migration during initial healing phases. The placement of these implants by using a flapless technique may lead to stable Marginal Bone Level (MBL). Aim/Hypothesis This preliminary study aims to evaluate soft and hard tissues modification around implants with Laser-Lok microtextured neck placed at tissue level with flapless technique. MBL and gingival parameters will be evaluated up to 6 months. Material and Methods Healthy, non smoker patients were enrolled in this prospective clinical study. Tapered tissue level implants (Biohorizon, Birmingham, AL, USA), characterized by 1.8 mm Laser-Lok neck were used. The implant shoulders were placed levelled with the soft tissues to obtain a tissue level insertion and using a flapless technique. Customized abutments and provisional crowns were applied after 3 months. Definitive metal-ceramic crowns were cemented after 4 months. Periapical Rx were taken at implant insertion, 1.3 months (pre-loading) and 6 months (post-loading). MBL was evaluated in single blind using Image J software (NIH, Bethesda,USA). Plaque score (PS) and Bleeding on Probing (BoP) will be monitored at 4 sites around the implant restoration (Mesial, Distal, Lingual, Vestibular) at 3 months (before prosthetic phases) and at 6 months. Dichotomous scores were used (0 = absence, 1 = presence) for both parameters. Gingival biotype (thin thick) was also recorded. Results A total of 15 implants were placed in 13 patients (9F+4M, mean age 59 ± 8 years). After 6 months, all implants were safe from complications. A thick gingival biotype was found in 8 implant sites, while thin biotype was found in 7. After 6 months, extremely low BoP was observed (4.16%) while PS was positive in 9.5% of the cases. Implant distribution and MBL according to operative parameters (gender, location, implant placement timing, diameter and gingival thickness) is reported in Table 1. Mean MBL (expressed as mm ± SD) was −0.07 ± 0.14 at 1 month, −0.15 ± 0.24 at 3 months and −0.26 ± 0.17 at 6 months. Conclusions and Clinical Implications Our preliminary data showed that tapered tissue level implants may be placed at tissue level by using a flapless approach. A stable crestal bone with minimal bone loss was observed during healing phases. Healthy soft tissues with low signs of inflammation were observed around Laser-Lok microtextured neck as confirmed by low BoP and PS.
Magnetic Resonance Imaging-Guided Biopsy in Active Surveillance of Prostate Cancer (2021)

When AS begins and follows with MRGBx (targeted and systematic), upgrading rate (≥GG3) is greater when tumor is initially present within a magnetic resonance imaging lesion or when pathology is GG2 than when these features are absent.
Dynamic Time Warping of Pulse Wave Curves (2018)

Abstract Being able to non-obtrusively and continuously monitor arterial blood pressure is of great interest, particularly in the context of wearable sensors. A common limitation is the need for dedicated hardware, which is either obtrusive or expensive. In our current work, we investigate unimodal pulse waves from three handily accessible heterogeneous sources: photoplethysmography, bioimpedance and pulse applanation tonometry. We derive and evaluate multiple parameters regarding their correlation to reference blood pressure curves. These parameters stem from features of the warping paths resulting from dynamic and derivative dynamic time warping. The warping is performed between adjacent pulses or to a reference waveform. Spearman Rho coefficients of up to 0.98 and averaging 0.77 at highly significant p-values are recorded for single parameters. We record mean absolute deviation values of 0.08 across subjects. The results indicate there are negligible lags between reference and parameter curves. The sign of the correlation coefficients is consistent only for a small subset of parameters; the underlying cause could not yet be identified. We conclude that the warping path approach seems a promising way to go, yet still needs refinement. In particular, developing a time and amplitude warping method is paramount. Since warping quantizes all the morphological changes in the pulse wave without fiducial point detection, it could become a powerful tool for future investigations.
A Novel Flash-based FPGA Technology with Deep Trench Isolation (2007)

A highly scalable flash-based Field Programmable Gate Array (FPGA) technology has been achieved with Deep Trench Isolation (DTI). The DTI allows for a reduced cell size and enables Independent Pwell (IPW) array operation. The IPW FPGA array operation requires less than + 10 V during Uniform Channel FN-FN programming and shows negligible gate disturb. Additionally, it eliminates Gate-Induced Drain Leakage (GIDL) during programming. Characterization of a FPGA cell and array with 90 nm design rules is demonstrated with excellent electrical characteristics.
TCP Servers: A TCP/IP offloading architecture for internet servers, using memory-mapped communication (2002)

TCP Server is a system architecture aiming to offload network processing from the host(s) running an Internet server. The TCP Server can be executed on a dedicated processor, node or intelligent network interface using low-overhead, non-intrusive communication between it and the host(s) running the server application. In this thesis, we present and evaluate an implementation of the TCP Server architecture for Internet servers on clusters built around a memory-mapped communication interconnect. We have quantified the impact of offloading on the performance of Internet servers for our TCP Server implementation, using a server application with realistic workloads. We were able to achieve performance gains of up to 30% due to offloading for the scenarios studied. Based on our experience and results, we conclude that offloading the network processing from the host processor using a TCP Server architecture is beneficial to server performance when the server is overloaded. A complete offloading of TCP/IP processing demands substantial computing resources on the TCP Server. Depending on the application workload, either the host processor, or the TCP Server, can become the bottleneck indicating the need for an adaptive scheme to balance the load between the host and the TCP Server.
Breast cancer-related mortality in Central and Eastern Europe: years of life lost and productivity costs (2023)

Premature death from BC leads to substantial YLL and productivity losses. Lost productivity costs due to premature BC-related mortality exceeded €259 million in 2019 alone. The data modeled provide important evidence toward resource allocation priorities for BC prevention, screening, and treatment that could potentially decrease productivity losses. Careful consideration should be given to BC-specific policies, such as surveillance programs and the availability of new treatments in CEE countries to decrease the medical and financial burden of the disease.
Supplementary Figure 2 from Stress-induced Norepinephrine Downregulates CCL2 in Macrophages to Suppress Tumor Growth in a Model of Malignant Melanoma (2023)

&lt;p&gt;Stress correlates with reduced tumor size and burden, VEGF and CCL2 levels, and infiltrating macrophages compared to unstressed mice.&lt;/p&gt;