gebhart/patrepeval huggingface dataset:
Liver delivery of NO by NCX‐1000 protects against acute liver failure and mitochondrial dysfunction induced by APAP in mice  THIS ARTICLE HAS BEEN RETRACTED (2004)

1. NCX-1000, (3alpha, 5beta, 7beta)-3,7-dihydroxycholan-24oic acid[2-methoxy-4-[3-[4-(nitroxy)butoxy]-3-oxo-1-propenyl]phenyl ester, is a nitric oxide (NO)-derivative of ursodeoxyxholic acid (UDCA) that selectively release NO in the liver. 2. Here, we demonstrated that administering mice with 40 micromol kg(-1) NCX-1000, but not UDCA, improves liver histopathology and reduces mortality caused by 330 micromol kg(-1) APAP from 60 to 25% (P<0.01). Administration of NCX-1000, in a therapeutic manner, that is, 2 h after acetaminophen (APAP) intoxication reduced mortality, improved liver histopathology and prevented liver IFN-gamma, TNF-alpha, Fas/Fas ligand and inducible nitric oxide synthase (iNOS) mRNA accumulation caused by APAP. 3. In vitro exposure of primary cultures of mouse hepatocytes to APAP, 6.6 mm, resulted in apoptosis followed by necrosis. Loss of cell viability correlates with early mitochondrial membrane potential (Deltapsi(m)) hyperpolarization followed by depolarization and cytochrome c translocation from mitochondria to cytosol. APAP-induced apoptosis associated with procaspase-3 and -9 cleavage, appearance of truncated Bid and activation of poly(ADP-ribose) polymerase (PARP). 4. Treating primary culture of hepatocytes with 5 microm cyclosporine and 10 microm trifluoperazine for eight resulted in significant reduction of apoptosis induced by APAP suggesting that loss of Deltapsim was mechanistically involved in apoptosis induced by APAP in vitro. 5. NCX-1000, but not UDCA, concentration-dependently (ED(50)=16 microm) protected against Deltapsi(m) depolarization and reduced transition from apoptosis to necrosis caused by 6.6 mm APAP. 6. Treating primary cultures of hepatocytes with the NO-donor DETA-NO, 100 microm, reduced apoptosis induced by APAP and prevented caspase activation. 7. In conclusion, NCX-1000 is effective in protecting against APAP-induced hepatotoxicity when administered in a therapeutic manner. This protection may involve the inhibition of apoptosis and the maintenance of mitochondrial integrity.
Canine Genetic Linkage Study Using Heterologous DNA Probes (1996)

We have used clones of 17 single-copy human DNA sequences to analyze their counterparts in the genome of the domestic dog by heterologous hybridization. Ten of the 17 sequences represented anchor loci proposed for comparative mammalian mapping. Eight of 17 human clones (including three of the anchor loci) gave clear hybridization signals when used with Southern blots of canine DNA. Five of these eight (including two anchor loci) showed diallelic restriction fragment length polymorphisms in a large kindred of Brittany spaniels and could be used for segregation studies. Several probes chosen from different human chromosomes also were unlinked in the dog. By contrast, linkage was found between the canine counterparts of the closely linked human serum amyloid A gene family. Three markers linked on human chromosome II appeared not to be syntenic in the dog. DNA markers linked to various human genetic neuromuscular diseases were not linked to hereditary canine spinal muscular atrophy which segregates in this kindred. However, there was evidence of possible linkage of this disorder with a canine counterpart of the tyrosinase gene. Segregation studies using heterologous single-copy DNA probes can be performed in dogs, but the level of inbreeding may reduce heterogeneity and limit the power of the analysis.
A membrane-anchored aptamer sensor for probing IFNγ secretion by single cells (2017)

Insight into the behavior of individual immune cells, in particular cytokine secretion, will contribute to a more fundamental understanding of the immune system. In this work, we have developed a cell membrane-anchored sensor for the detection of cytokines secreted by single cells using a combination of aptamer-based sensors and droplet microfluidics.
A lateralized tapered titanium cementless femoral component does not increase thigh or trochanteric pain. (2007)

Increased femoral component lateralization has been reported to recreate femoral offset accurately, and should provide better function. Concerns exist that negative effects may occur such as increased thigh pain, trochanteric bursitis, and loosening. The current study aims to examine whether a lateralized femoral component design is associated with increase in thigh pain, trochanteric pain, or implant failure when used to recreate hip soft tissue balance and stability. The authors reviewed 98 total hip arthroplasties (THA) using the Mallory-Head Porous femoral component. Group 1 had 49 consecutive THA performed before the lateralized stem was available. Group 2 had 49 consecutive THA in which lateralization was deemed necessary, based upon preoperative templating and intraoperative soft tissue balancing. Follow up averaged 46 and 38 months, respectively. No stems failed. Group 1 had three dislocations and Group 2 had none with increased offset. Six (12%) patients in Groups 1 and 3 (6%) patients in Group 2 had trochanteric pain (p<0.05). Three (6%) patients had moderate thigh pain in Group 1 and none in Group 2. Significantly less trochanteric and thigh pain was observed in those patients in whom a lateralized stem was deemed necessary. The use of a lateralized stem improves the accuracy of hip soft tissue reconstruction and does not increase thigh pain, trochanteric pain, or loosening. Accurate soft tissue reconstruction may decrease trochanteric and thigh pain and improve function following THA.
Formation and crystal structure of [ClPtNMe2NHCH2CHPPh2CH2CH2PPh2]Cl containing a novel tridentate ylide ligand formed by intramolecular phosphinovinylation (1987)

Treatment of cis-[PtCl2(PPh2CHCH2)2] with Me2NNH2 gives the title compound, formed by successive conjugate additions to the vinyl groups, including attack by one P-atom on a vinyl group of an adjacent co-ordinated PPh2CHCH2 ligand: some reactions of the title compound are described.
Alpha-Fetoprotein Response After Locoregional Therapy for Hepatocellular Carcinoma: Oncologic Marker of Radiologic Response, Progression, and Survival (2009)

The data presented support the use of AFP response seen after locoregional therapy as an ancillary method of assessing tumor response and survival, as well as an early objective screening tool for progression by imaging.
Improved Adhesion of Thin Conformal Films to Metal Surfaces. (1986)

A technique is described for attaching thin, conformal, pin-hole free electrically insulating polyethylene films to flat gold surfaces (previously modified by adsorption of a monolayer of an organic disulfide) by plasma polymerization. These polyethylene films are tough enough to support the attachment of gold electrodes. The adhesion of plasma polymerized polyethylene (PPE) thin films to a gold surface can be dramatically improved by coupling the synthesis of the PPE thin film with the prior assembly of an organic monolayer chemisorbed to the metal surface. These thin film assemblies have good physical characteristics and are stable to the ambient environment for long intervals. This method should be applicable to other systems with appropriate modifications.
Abstract C148: SC144: The first orally active small molecule gp130 inhibitor for the treatment of ovarian cancer. (2011)

Abstract Ovarian cancer is one of the leading causes of cancer-related death in women in the United States. The gp130/Stat3 signaling axis plays an important role in carcinogenesis and drug resistance in a wide variety of human cancers, including ovarian cancer. As a downstream effector of gp130, Stat3 is constitutively activated and plays a critical role in ovarian cancer progression. In this study, we showed that a novel selective small molecule gp130 inhibitor, SC144, suppressed ovarian cancer progression both in vitro and in vivo. SC144 treatment in vitro induced gp130 phosphorylation and deglycosylation, resulting in the downregulation of surface-bound gp130 and the abrogation of gp130-associated Stat3 activation. In addition, SC144 selectively inhibited the downstream signaling activation induced by gp130 substrates, including IL-6 and LIF, whereas SC144 did not exhibit detectable effects on the downstream signaling activation induced by non-gp130 substrates, including IFN-γ, SDF-1α, and PDGF. Protein expression regulated by the gp130/Stat3 axis in OVCAR-8 cells was also down-regulated after SC144 treatment, including Bcl-2, Bcl-XL, survivin, cyclin D1, MMP-7, gp130 and Ape1/Rel-1. In vivo, SC144 significantly inhibited tumor growth in a mouse xenograft model of human ovarian cancer via i.p. or p.o. administration. After SC144 treatment for two months, gp130, Bcl-2, Bcl-XL, MMP-7 and Ape1/Ref-1 protein levels were substantially decreased in the tumor site in the treatment group compared with the control group. Our results further suggest gp130 is a novel drug target for ovarian cancer therapy, and the development of small molecule inhibitors of gp130 can help interrogate this unique signaling pathway in human diseases. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the AACR-NCI-EORTC International Conference: Molecular Targets and Cancer Therapeutics; 2011 Nov 12-16; San Francisco, CA. Philadelphia (PA): AACR; Mol Cancer Ther 2011;10(11 Suppl):Abstract nr C148.
Expression of Carbonic Anhydrase V in Pancreatic Beta Cells Suggests Role for Mitochondrial Carbonic Anhydrase in Insulin Secretion (1998)

Carbonic anhydrase V (CA-V) is a mitochondrial enzyme that provides bicarbonate for pyruvate carboxylase in liver and kidney. In the course of a survey of the tissue distribution of CA-V, we detected intense immunostaining in pancreatic islets when sections from rat and mouse pancreases were reacted with a polyclonal antibody to recombinant mouse CA-V. The distribution and large number of CA-V-positive cells in each islet suggested that they represented beta cells. Double immunofluorescence staining of tissue sections and isolated islet cells showed cellular colocalization of CA-V and insulin, confirming that beta cells contain CA-V. Western blotting of rat islets of Langerhans and primary beta cells showed 33- and 30-kDa polypeptides of precursor and mature CA-V, respectively. The CA-V expression was beta cell-specific since no CA-V immunoreaction was detected in the primary alpha cells. Immunohistochemical staining for CA-I, CA-II, CA-IV, CA-VI, and CA-IX was negative in beta cells, and Western blotting of beta cells also failed to identify any CA in beta cells except CA-V. The specific localization of CA-V in beta cells led us to hypothesize that CA-V may be functionally linked to the regulation of insulin secretion. Consistent with this hypothesis, the CA inhibitor acetazolamide was found to be a strong inhibitor of glucose-stimulated insulin secretion by isolated rat pancreatic islets.
Interactions between flow fields induced by surface dielectric barrier discharge arrays (2023)

This study investigates the flow field induced by a surface dielectric barrier discharge (SDBD) system, known for its efficient pollution remediation of volatile organic compounds (VOCs). We aim to understand the flow dynamics that contribute to the high conversion observed in similar systems. Experimental techniques, including schlieren imaging and particle image velocimetry (PIV), applied with high temporal resolution, were used to analyse the flow field. Complementary, fluid simulations are employed to investigate the coupling between streamer and gas dynamics. Results show distinct fluid field behaviours for different electrode configurations, which differ in geometric complexity. The fluid field analysis of the most basic electrode design revealed behaviours commonly observed in actuator studies. The simulation results indicate the local information about the electron density as well as different temporal phases of the fluid flow. The electrode design with mostly parallel grid line structures exhibits confined vortices near the surface. In contrast, an electrode design also used in previous studies, is shown to promote strong gas transport through extended vortex structures, enhancing gas mixing and potentially explaining the high conversion observed.
Apolipoproteins A1, B, and apoB/apoA1 ratio are associated with first ST-segment elevation myocardial infarction but not with recurrent events during long-term follow-up (2018)

In conclusion, apoA1, apoB, and apoB/apoA1 were strongly associated with risk of STEMI. No clear relation between VLDL-associated apos and the risk of STEMI was found. Neither baseline serum apos nor lipids predicted MACE in statin-treated patients during long-term follow-up after a first STEMI.
Adhesive Interactions Between Human Neural Stem Cells and Inflamed Human Vascular Endothelium Are Mediated by Integrins (2006)

Understanding the mechanisms by which stem cells home precisely to regions of injury or degeneration is of importance to both basic and applied regenerative medicine. Optimizing regenerative processes may depend on identifying the range of molecules that subserve stem cell trafficking. The "rolling" of extravasating cells on endothelium under conditions of physiological flow is the first essential step in the homing cascade and determines cell adhesion and transmigration. Using a laminar flow chamber to simulate physiological shear stress, we explored an aspect of this process by using human neural stem cells (hNSCs). We observed that the interactions between hNSCs and tumor necrosis factor-alpha (TNF-alpha)-stimulated human endothelium (simulating an inflamed milieu) are mediated by a subclass of integrins--alpha2, alpha6, and beta1, but not alpha4, alphav, or the chemokine-mediated pathway CXCR4-stromal cell-derived factor-1alpha--suggesting not only that the mechanisms mediating hNSC homing via the vasculature differ from the mechanisms mediating homing through parenchyma, but also that each step invokes a distinct pathway mediating a specialized function in the hNSC homing cascade. (TNF-alpha stimulation also upregulates vascular cell adhesion molecule-1 expression on the hNSCs themselves and increases NSC-endothelial interactions.) The selective use of integrin subgroups to mediate homing of cells of neuroectodermal origin may also be used to ensure that cells within the systemic circulation are delivered to the pathological region of a given organ to the exclusion of other, perhaps undesired, organs.
Challenges for the application of material flow analysis at the level of production processes – case study: Blast furnace process (2016)

The method of material flow analysis (MFA) is used to trace flows and stocks of goods and substances within different systems, such as cities and regions, enterprises and industrial processes. However, detailed MFA on an enterprise level hardly exist. The goal of the present paper is to apply MFA on a blast furnace process and to discuss the challenges arising. In particular, heavy metal flows (Chromium, Mercury) through the process are modelled. In a first step transfer coefficients (TC) of the heavy metals have been determined for one particular year (2009) and checked for plausibility. In the second step, these TC have been applied to simulate the heavy metal flows in the outputs of the blast furnace process for the year 2011. These flows have subsequently been compared with the outputs measured. The results show that the application of TC for Cr delivers plausible results for the output flows. Also for Hg the application of the TC seems to be justified, although a detailed investigation of the Hg balance indicates significant differences between the total input and output of Hg. As the amount of Hg detected in the output is larger than in the input (mass balance error greater than 80%), this difference originates most likely from non-detectable Hg contents (concentrations below the limit of quantification) in different iron bearing input flows.
Morphometric Characterization of an Ex Vivo Porcine Model of Functional Tricuspid Regurgitation (2022)

Emerging treatments for tricuspid valve (TV) regurgitation require realistic TV pathological models for preclinical testing. The aim of this work was to investigate structural features of fresh and defrosted porcine right-heart samples as models of mild and severe functional tricuspid regurgitation (FTR) condition in ex-vivo pulsatile flow platform. Ten fresh hearts were tested ex-vivo under steady and pulsatile flow in typical right-heart loading conditions. Hemodynamics and 3D echocardiographic imaging of TV and right ventricle (RV) were acquired. Hearts were then kept frozen for 14 days, defrosted, and tested again with the same protocol. Morphometric parameters of TV and RV were derived from 3D reconstructions based on echo data. Fresh samples showed a slightly dilated TV morphology, with coaptation gaps among the leaflets. Sample freezing induced worsening of TV insufficiency, with significant (p < 0.05) increases in annulus size (annulus area and perimeter 7.7-3.1% respectively) and dilation of RV (9.5%), which led to an increase in tenting volume (123.7%). These morphologic alterations reflected into a significant increment of regurgitation fraction (27%). Together, such results suggest that fresh porcine heart samples may be a reliable ex-vivo model of mild FTR condition, which can be enhanced through freezing/thawing treatment to model a severe pathological condition.
Laser-Induced Freezing (1985)

Laser beams are crossed in a two-dimensional colloidal liquid of strongly interacting spherical particles to produce an external periodic potential and stimulate a density modulation. If the modulation wave vector is at the peak in the static structure factor, a sufficiently large potential induces a phase transition to a structure which exhibits solidlike order having density modes other than those directly excited. A Landau theory is presented and discussed in terms of our results.
Load transfer between femur and prosthesis following total hip replacement. Experimental and analytical study on the effect of the cement property. (1990)

We investigate here the load transfer between femur and prosthesis following total hip replacement. First, compression tests using a human cadaveric femur are conducted. Then plaster models for the femur are made from the molds taken from the femur. We believe thet the plaster model femur can allow us to distinguish the minute differences affected from experimental setups. Utilizing the five kinds of cementing materials and the two kinds of prosthesis stems, the effects of the modulus of the cementing materials to the strain distribution on the model femur are examined. Further, a numerical analysis method based on the beam theory is proposed, and the load transfer problems between femur and prosthesis are solved by the Runge-Kutta method. The numerical results obtained are in good agreement with the experimental results, which indicates that the distal load transfer between femur and prosthesis takes place in a manner similar to that between two beams. Both the experimental and analytical results predict that the strain distribution induced on the femur with implanted prosthesis differs from the physiological one considerably; i. e. the strain at the calcar is maximum before implantation whereas the strain at the distal point become dominant after implantation. It may be concluded that the stress shielding can't be avoided as long as the presently available prostheses are utilized.
Visual Performance and Optical Quality after Implantation of a New Generation Monofocal Intraocular Lens (2021)

ICB00 provided superior intermediate vision and comparable distance performance and photic phenomena compared to a standard monofocal IOL.
On image rendering methods for improved image consistency in PACS environment (2010)

The average workload per full-time equivalent (FTE) radiologist increased by 70% from1991-1992 to 2006- 2007. The increase is mainly due to the increase (34%) in the number of procedures, particularly in 3D imaging procedures. New technologies such as picture archiving and communication systems (PACS) and embodied viewing capability were accredited for an improved workflow environment leading to the increased productivity. However, the need for further workflow improvement is still in demand as the number of procedures continues growing. Advanced and streamlined viewing capability in PACS environment could potentially reduce the reading time, thus further increasing the productivity. With the increasing number of 3D image procedures, radiographic procedures (excluding mammography) have remained their critical roles in screening and diagnosis of various diseases. Although radiographic procedures decreased in shares from 70% to 49.5%, the total number has remained the same from 1991-1992 to 2006- 2007. Inconsistency in image quality for radiographic images has been identified as an area of concern. It affects the ability of clinicians to interpret images effectively and efficiently in areas where diagnosis, for example, in screening mammography and portable chest radiography, requires a comparison of current images with priors. These priors can potentially have different image quality. Variations in image acquisition techniques (x-ray exposure), patient and apparatus positioning, and image processing are the factors attributed to the inconsistency in image quality. The inconsistency in image quality, for example, in contrast may require manual image manipulation (i.e., windowing and leveling) of images to accomplish an optimal comparison to detect the subtle changes. We developed a tone-scale image rendering technique which improves the image consistency of chest images across time and modality. The rendering controls both the global and local contrast for a consistent look. We expect the improvement could reduce the window and level manipulation time required for an optimal comparison of priors and current images, thus improving both the efficiency and effectiveness of image interpretation. This paper presents a technique for improving the consistency of portable chest radiographic images. The technique is based on regions-of-interest (ROIs) to control both the local and global contrast consistency.
[How I treat… a pelvic organ prolapse at the doctor's office]. (2018)

The use of gynecological pessary to put back up prolapsed organs to their normal position appears to be as effective as surgical management to relieve symptoms related to uro-genital prolapse and restore body image. This millenary device can be used temporarily, awaiting a surgical solution or as a therapeutic test (mimicking the effect of a surgical procedure to predict its functional outcome or identifying a masked urinary incontinence). It can also represent an alternative to surgery (patient choice, women who wish to complete childbearing or who are unsuitable for surgery because of medical comorbidities) and thus can be used in first intention. Pessary can also be used to confirm or deny the responsibility of pelvic organ prolapse for atypical symptomatology as pelvic heaviness or pain. However, this use requires adjuvant treatment. In this paper, we highlight an original effective process not yet published to optimize the tolerance of pessary.
Measuring Random Microscopic Motion of Water in Tissues with MR Imaging (1991)

Cat brain images sensitized to incoherent motion by additional gradient pulses were obtained on a 4.7 T magnetic resonance unit equipped with shielded gradient coils. The apparent diffusion coefficient of water in gray and white matter was accurately determined and imaged from the signal attenuation curve obtained as a function of gradient strength. Contrast in calculated diffusion images differed from typical T2-weighted contrast. Furthermore, in gray matter and in areas containing flowing CSF the attenuation curve was found to be biexponential. These results are interpreted in terms of a simple voxel model with microcirculation and diffusion contributions.