gebhart/patrepeval huggingface dataset:
Synthesis of spirocycles via ring closing metathesis of heterocycles carrying gem-diallyl substituents obtained via ring opening of (halomethyl)cyclopropanes with allyltributyltin (2003)

In the presence of allyl tri-n-butyltin--AIBN, cyclopropylmethyl bromides/xanthates undergo ring-opening reaction with concomitant formation of geminal diallyl derivatives in good yields. The ring closing metathesis reactions on geminal diallyl derivatives with Grubbs' catalyst provided spirocyclopentenyl products. Combination of these two methodologies has been applied to the synthesis of mono-, bis-cyclopentyl-carbohydrates as well as spirocyclopentylproline derivatives.
Machining chatter in continuous facing under a constant nominal cutting speed: Tool vibration with time-varying delay (2015)

This article studies regenerative chatter in single-point face-machining at nominally constant speed under continuous conditions. A temporal model for rotational speed was developed and experimentally verified. The resulting rotational time-delay was cast into the classical force feedback mechanism for chatter. A chatter model was formulated to allow slight spindle speed variation about the temporal model. The modified method of steps was then employed to solve the tool vibration in time-domain allowing one (single-degree-of-freedom) or two (2-degree-of-freedom model) vibrational modes. Exploratory facing experiments using a grooving tool were conducted on a nickel alloy workpiece. It was found that the tool was more susceptible to chatter at larger diameters. It appeared that the single-degree-of-freedom model captured the most relevant of the observed phenomena while cutting without spindle speed variation, however, neither the 1-degree-of-freedom nor the 2-degree-of-freedom models could effectively capture the experimentally observed chatter evolution characteristics while cutting with spindle speed variation.
Influenza D pseudotyped lentiviruses: production, neutralisation assay and serological surveillance (2020)

Influenza D virus (IDV) has been reported in many animal species and potentially humans worldwide. Cattle are considered the major reservoir. There are currently three main lineages based on the haemagglutinin-esterase (HEF) gene: D/OK, D/660 and D/Japan. We performed pilot surveillance for IDV by using pseudotyped lentivirus (PVs) to generate a cell-based test to identify prior-exposure to IDV in animals. The expression plasmids of the HEF genes, D/swine/Italy/2015, D/bovine/France/2014, and D/bovine/Ibaraki/2016, were constructed. The HEF plasmid was co-transfected with lentiviral vector plasmid expressing luciferase, lentiviral Gag-Pol plasmid, and HAT protease plasmid in producer cells (HEK293T/17). Three days post-transfection, supernatants were collected and used for titration on various cell lines and in micro-neutralisation tests. Sera from pigs vaccinated with D/swine/Italy/2015 and D/swine/Oklahoma/2011 were used to undertake a preliminary validation of the micro-neutralisation assay. All pig sera have neutralising activity to influenza D (Italy) pseudotyped lentiviruses. Cow and sheep sera, 145 and 114 specimens, respectively, collected from UK farms were screened using the micro-neutralisation test. We found 97 bovine sera (66.9%) were influenza D antibody positive. Collectively, pseudotyped lentivirus technology opens up opportunities for serological surveillance of influenza D viruses.
Matrix Development for the Detection of Phosphorylated Amyloid-β Peptides by MALDI-TOF-MS (2023)

Amyloid-β (Aβ) peptides, including post-translationally modified variants thereof, are believed to play a key role in the onset and progression of Alzheimer's disease. Suggested modified Aβ species with potential disease relevance include Aβ peptides phosphorylated at serine in position eight (pSer8-Aβ) or 26 (pSer26-Aβ). However, the published studies on those Aβ peptides essentially relied on antibody-based approaches. Thus, complementary analyses by mass spectrometry, as shown for other modified Aβ variants, will be necessary not only to unambiguously verify the existence of phosphorylated Aβ species in brain samples but also to reveal their exact identity as to phosphorylation sites and potential terminal truncations. With the aim of providing a novel tool for addressing this still-unresolved issue, we developed a customized matrix formulation, referred to as TOPAC, that allows for improved detection of synthetic phosphorylated Aβ species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. When TOPAC was compared with standard matrices, we observed higher signal intensities but minimal methionine oxidation and phosphate loss for intact pSer8-Aβ(1-40) and pSer26-Aβ(1-40). Similarly, TOPAC also improved the mass spectrometric detection and sequencing of the proteolytic cleavage products pSer8-Aβ(1-16) and pSer26-Aβ(17-28). We expect that TOPAC will facilitate future efforts to detect and characterize endogenous phosphorylated Aβ species in biological samples and that it may also find its use in phospho-proteomic approaches apart from applications in the Aβ field.
Supplemental Figure 5 from Herbacetin Is a Novel Allosteric Inhibitor of Ornithine Decarboxylase with Antitumor Activity (2023)

&lt;p&gt;The effect of herbacetin on AP-1 upstream proteins. Colon cancer cells were treated with herbacetin for 48 h and expression of total and phosphorylated ERKs and RSK and total ODC was determined by Western blotting. Beta-Actin was used as a loading control. Similar results were obtained from 3 independent experiments and representative blots are shown.&lt;/p&gt;
Rapid Peptide Turnover and Inefficient Presentation of Exogenous Antigen Critically Limit the Activation of Self-Reactive CTL by Dendritic Cells (2001)

This study evaluated to what extent presentation of exogenously acquired self-Ags via MHC class I molecules on DC might contribute to the activation of self-reactive CTL and subsequent development of autoimmune disease. We show here by using the rat insulin promotor lymphocytic choriomeningitis virus glycoprotein model of autoimmune diabetes that the activation of self-reactive CTL by DC after uptake of exogenous Ag is very limited, first by the short half-life of MHC class I-associated peptides on DC in vitro and in vivo, and second by the rather inefficient MHC class I presentation of cell-associated self-Ags by DC. These two mechanisms are probably crucial in establishing high thresholds for the induction of self-reactive CTL that prevent autoimmune sequelae after release of sequestered and previously immunologically ignored tissue Ags.
Tensile Properties, Collagen Content, and Crosslinks in Connective Tissues of the Immature Knee Joint (2011)

To our knowledge, this study is the first to examine tensile properties, collagen content, and pyridinoline crosslink abundance in a direct head-to-head comparison among all of the major connective tissues of the knee. This is also the first study to report results for pyridinoline crosslink density that suggest its preferential role over collagen in determining tensile stiffness for certain tissues.
Achieving Robust Mobile Web Content Delivery Performance Based on Multiple Coordinated QUIC Connections (2018)

In order to minimize the downloading time of short-lived applications like Web browsing, Web application, and short video clips, the recently standardized HTTP/2 adopts stream multiplexing on one single TCP connection. However, aggregating all content objects within one single connection suffers from the head-of-line blocking issue. Quick UDP Internet connection (QUIC), by eliminating such an issue on the basis of UDP, is expected to further reduce the content downloading time. However, in mobile network environments, the single connection strategy still leads to a degraded and high-variant completion time due to the unexpected hindrance of congestion window growth caused by the common but uncertain fluctuations in round trip time and also random loss event at the air interface. To retain resilient congestion window against such network fluctuations, we propose an intelligent connection management scheme based on QUIC which not only employs adaptively multiple connections but also conducts a tailored state and congestion window synchronization between these parallel connections upon the detection of network fluctuation events. According to the performance evaluation results obtained from an LTE-A/Wi-Fi testing network, the proposed multiple QUIC scheme can effectively overcome the limitations of different congestion control algorithms (e.g., the loss-based new reno/CUBIC and the rate-based BBR), achieving substantial performance improvement in both median (up to 59.1%) and 95th completion time (up to 72.3%). The significance of this piece of work is to achieve highly robust short-lived content downloading performance against various uncertainties of network conditions as well as with different congestion control schemes.
A database for handwriting recognition research in Sinhala language (2005)

This article presents a database of images ofhandwritten city names. The aim is to provide a standarddatabase for Sinhala handwriting recognition research.This database contains about 15,000 images of about 500city names of Sri Lanka. These images are obtained fromthe addresses of live mail so that the writers had no ideathat they would be used for this purpose. Also, these areunconstrained handwriting images unlike the imagescollected using prescribed forms in laboratoryenvironment. The images are divided into two groups,training set and testing set. This enables the comparisonof results of different researches and serves the purposeof being a standard database.
Characterization of milk and soy phospholipid liposomes and inflammation in 3T3-L1 adipocytes (2021)

Milk phospholipids (PL) are valuable dairy components that appear to impart human health benefits, including improved cognitive function in infants and adults. The commercial food industry uses primarily plant-based sources of PL, such as soy lecithin. However, it remains unclear whether different compositions of PL from different dietary sources, such as milk, convey the same benefits. We hypothesized that PL derived from bovine milk or soy have differing physiological effects in terms of inflammation due to their differences in composition. The objectives of this study were to characterize milk and soy liposomes by their physicochemical properties and composition and to evaluate their effects in vitro by means of inflammatory gene expression analyses. Milk and soy phospholipid large unilamellar vesicles (MPL-LUV and SPL-LUV, respectively) prepared using thin-film hydration coupled with extrusion were similar in terms of structure, size, and stability; however, they differed significantly in composition. The 3T3-L1 adipocytes were selected for this work because adipocytes are the main site of uptake, synthesis, modification, and breakdown of lipids and are important inflammatory mediators in mammalian systems. In this work, these cells exposed to both liposome varieties showed high biocompatibility and low cytotoxicity up to concentrations of 0.5 mg/mL as measured by colorimetric MTT and lactate dehydrogenase assays. Furthermore, SPL-LUV showed trends toward stimulating inflammation compared with MPL-LUV as measured by expression of 2 proinflammatory cytokines, monocyte chemoattractant protein-1 (MCP-1) and IL-6. Expression of MCP-1 significantly increased 1.82-fold relative to the control upon SPL-LUV treatment, with similar trends for IL-6 (increased 1.59-fold). The MPL-LUV showed relatively no change in cytokine expression. The results obtained in this work suggest that the methodology used to prepare LUV and the composition and proportion of milk PL are important in measuring cell physiology changes and inflammatory status in mammalian cells.
Chelating N‐Heterocyclic Carbene Ligands Enable Tuning of Electrocatalytic CO<sub>2</sub> Reduction to Formate and Carbon Monoxide: Surface Organometallic Chemistry (2018)

Reported here is the chelate effect as a design principle for tuning heterogeneous catalysts for electrochemical CO<sub>2</sub> reduction. Palladium functionalized with a chelating tris-N-heterocyclic carbene (NHC) ligand (Pd-timtmb<sup>Me</sup> ) exhibits a 32-fold increase in activity for electrochemical reduction of CO<sub>2</sub> to C1 products with high Faradaic efficiency (FE<sub>C1</sub> =86 %) compared to the parent unfunctionalized Pd foil (FE=23 %), and with sustained activity relative to a monodentate NHC-ligated Pd electrode (Pd-mimtmb<sup>Me</sup> ). The results highlight the contributions of the chelate effect for tailoring and maintaining reactivity at molecular-materials interfaces enabled by surface organometallic chemistry.
A Planar Dual-Polarized Microstrip 1-D-Beamforming Antenna Array for the 24-GHz Band (2016)

A planar linearly dual-polarized mm-wave antenna array is designed, fabricated, and investigated in detail. The requirements are two orthogonal polarizations with good purity and suitability of the array column for 1-D beamforming applications. Since single-layer fabrication without using vertical interconnection accesses is another important requirement, the chosen radiating elements are square microstrip patches, fed at two orthogonal edges. The array feeding is designed in series microstrip line fashion, providing a highly compact structure. In that way, 1-D beamforming agility is gained, when multiple array columns are arranged side by side and the individual columns are fed with appropriate phases. The sidelobe level of the array columns is decreased by implementing an amplitude taper within the series array, following the Dolph-Chebychev distribution. A fast scattering parameter calculation procedure is presented to achieve an efficient simulation and design process. The simulated and measured antenna properties are very stable over a bandwidth of 500 MHz around the center frequency proving that such a challenging dual-polarized design can be successfully implemented at millimeter-wave (mm-wave) frequencies with just one metallization layer on a grounded substrate. The design parameters and the design procedure are demonstrated in a step by step manner to give sufficient insight into the process together with the possibility of reusing the antenna array itself, as well as the underlying concepts.
Aeroacoustics of a low Mach tip gap flow (2008)

International audience
Feedback Active Noise Control System Combining Linear Prediction Filter (2010)

Publication in the conference proceedings of EUSIPCO, Aalborg, Denmark, 2010
Two Subtle Amino Acid Changes in a Transaminase Substantially Enhance or Invert Enantiopreference in Cascade Syntheses (2015)

Amine transaminases (ATAs) are powerful enzymes for the stereospecific production of chiral amines. However, the synthesis of amines incorporating more than one stereocenter is still a challenge. We developed a cascade synthesis to access optically active 3-alkyl-substituted chiral amines by combining two asymmetric synthesis steps catalyzed by an enoate reductase and ATAs. The ATA wild type from Vibrio fluvialis showed only modest enantioselectivity (14 % de) in the amination of (S)-3-methylcyclohexanone, the product of the enoate-reductase-catalyzed reaction step. However, by protein engineering we created two variants with substantially improved diastereoselectivities: variant Leu56Val exhibited a higher R selectivity (66 % de) whereas the Leu56Ile substitution caused a switch in enantiopreference to furnish the S-configured diastereomer (70 % de). Addition of 30 % DMSO further improved the selectivity and facilitated the synthesis of (1R,3S)-1-amino-3-methylcyclohexane with 89 % de at 87 % conversion.
Right ventromedial prefrontal lesions result in paradoxical cardiovascular activation with emotional stimuli (2006)

Ventromedial prefrontal cortex (VMPFC) lesions can alter emotional and autonomic responses. In animals, VMPFC activation results in cardiovascular sympathetic inhibition. In humans, VMPFC modulates emotional processing and autonomic response to arousal (e.g. accompanying decision-making). The specific role of the left or right VMPFC in mediating somatic responses to non-arousing, daily-life pleasant or unpleasant stimuli is unclear. To further evaluate VMPFC interaction with autonomic processing of non-stressful emotional stimuli and assess the effects of stimulus valence, we studied patients with unilateral VMPFC lesions and assessed autonomic modulation at rest and during physical challenge, and heart rate (HR) and blood pressure (BP) responses to non-stressful neutral, pleasant and unpleasant visual stimulation (VES) via emotionally laden slides. In 6 patients (54.0 +/- 7.2 years) with left-sided VMPFC lesions (VMPFC-L), 7 patients (43.3 +/- 11.6 years) with right-sided VMPFC lesions (VMPFC-R) and 13 healthy volunteers (44.7 +/- 11.6 years), we monitored HR as R-R interval (RRI), BP, respiration, end-tidal carbon dioxide levels, and oxygen saturation at rest, during autonomic challenge by metronomic breathing, a Valsalva manoeuvre and active standing, and in response to non-stressful pleasant, unpleasant and neutral VES. Pleasantness versus unpleasantness of slides was rated on a 7-point Likert scale. At rest, during physical autonomic challenge, and during neutral VES, parameters did not differ between the patient groups and volunteers. During VES, Likert scores also were similar across the three groups. During pleasant and unpleasant VES, HR decreased (i.e. RRI increased) significantly whereas BP remained unchanged in volunteers. In VMPFC-L patients, HR decrease was insignificant with pleasant and unpleasant VES. BP slightly increased (P = 0.06) with pleasant VES but was stable with unpleasant VES. In contrast, VMPFC-R patients had significant increases in HR and BP during pleasant and not quite significant HR increases (P = 0.06) with only slight BP increase during unpleasant VES. Other biosignals remained unchanged during VES in all groups. Our results show that VMPFC has no major influence on autonomic modulation at rest and during non-emotional, physical stimulation. The paradoxical HR and BP responses in VMPFC-R patients suggest hemispheric specialization for VMPFC interaction with predominant parasympathetic activation by the left, but sympathetic inhibition by the right VMPFC. Valence of non-stressful stimuli has a limited effect with more prominent left VMPFC modulation of pleasant and more right VMPFC modulation of unpleasant stimuli. The paradoxical sympathetic disinhibition in VMPFC-R patients may increase their risk of sympathetic hyperexcitability with negative consequences such as anxiety, hypertension or cardiac arrhythmias.
Vibration Energy Harvesting Using Multi-Stable Two Degree-of-Freedom Nonlinear Dynamics (2013)

In this research, we studied the integrated dynamics of a coupled linear oscillator-bistable energy harvester system. The method of harmonic balance together with perturbation analysis are used to predict the existence and stability of the bistable device interwell vibration. The influences of important parameters on tailoring the coupled system response are investigated to determine principles for improved energy harvesting performance. We demonstrate analytically that for excitation frequencies in a bandwidth less than the natural frequency of the linear oscillator, the additional linear system may substantially intensify the bistable harvester power generation as compared to a single bistable device. Moreover, the linear-bistable coupled system may introduce a stable out-of-phase dynamic between the linear and bistable elements around the resonance of the linear oscillator, enhancing the performance of the harvester by providing for a second interwell response. Key analytical findings are confirmed through experiments, validating the predicted trends and demonstrating the advantages of the coupled system for energy harvesting.
Performance-driven placement for design of rotation and right arithmetic shifters in monolithic 3D ICs (2013)

Recent advances in three-dimensional integrated circuits (3D-ICs) offer a new dimension of design exploration at traditional physical architecture of datapath components. The emerging monolithic inter-tier vias (MIVs) provides more advantages over through-silicon vias (TSVs) in terms of higher integration density and lower design overhead. In this work, we develop a performance-driven framework which uses simulated annealing to produce gate-level 3D placement layout for rotation shifter and right arithmetic shifter design. Compared to the optimum 2D layout, the critical path of our solution is much shorter with limited overhead on total wirelength. Our work indicates that by gatelevel 3D-IC integration, the new physical dimension can be well leveraged with improvement on both performance and power of shifter design.
Data from Generation of Tumor Antigen-Specific T Cell Lines from Pediatric Patients with Acute Lymphoblastic Leukemia—Implications for Immunotherapy (2023)

&lt;div&gt;Abstract&lt;p&gt;&lt;b&gt;Purpose:&lt;/b&gt; Although modern cure rates for childhood acute lymphoblastic leukemia (ALL) exceed 80%, the outlook remains poor in patients with high-risk disease and those who relapse, especially when allogeneic hematopoietic stem cell transplantation is not feasible. Strategies to improve outcome and prevent relapse are therefore required. Immunotherapy with antigen-specific T cells can have antileukemic activity without the toxicities seen with intensive chemotherapy, and therefore represents an attractive strategy to improve the outcome of high-risk patients with ALL. We explored the feasibility of generating tumor antigen-specific T cells &lt;i&gt;ex vivo&lt;/i&gt; from the peripheral blood of 50 patients with ALL [26 National Cancer Institute (NCI) high-risk and 24 standard-risk] receiving maintenance therapy.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Experimental Design:&lt;/b&gt; Peripheral blood mononuclear cells were stimulated with autologous dendritic cells pulsed with complete peptide libraries of WT1, Survivin, MAGE-A3, and PRAME, antigens frequently expressed on ALL blasts.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Results:&lt;/b&gt; T-cell lines were successfully expanded from all patients, despite low lymphocyte counts and irrespective of NCI risk group. Antigen-specificity was observed in more than 50% of patients after the initial stimulation and increased to more than 90% after three stimulations as assessed in IFN-γ-enzyme-linked immunospot (ELISpot) and &lt;sup&gt;51&lt;/sup&gt;Cr-release assays. Moreover, tumor-specific responses were observed by reduction of autologous leukemia blasts in short- and long-term coculture experiments.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Conclusion:&lt;/b&gt; This study supports the use of immunotherapy with adoptively transferred autologous tumor antigen-specific T cells to prevent relapse and improve the prognosis of patients with high-risk ALL. &lt;i&gt;Clin Cancer Res; 19(18); 5079–91. ©2013 AACR&lt;/i&gt;.&lt;/p&gt;&lt;/div&gt;
ChemInform Abstract: STEREOSPECIFIC ACCESS TO BRANCHED‐CHAIN CARBOHYDRATE SYNTHONS (1981)

Abstract Aus dem Ketozucker (I) erhält man durch Addition von HCN und Mesylierung die isomeren Addukte (IIa) und (IIb), aus (III)‐ entstehen über den Ketozucker (IV) analog die Isomeren (Va) und (Vb).
Technical Perspective: Catching lies (and mistakes) in offloaded computation (2016)

No abstract available.
Supplementary Figure 2 from CD73-Deficient Mice Are Resistant to Carcinogenesis (2023)

&lt;p&gt;PDF file - 3.5MB&lt;/p&gt;
β-Adrenergic Regulation of α <sub>3</sub> -Adrenergic Receptors in the Central Nervous System (1980)

Incubation of rat cerebral cortical slices with the beta-adrenergic agonist isoproterenol causes an increase in alpha 2-adrenergic receptor binding in addition to a decrease in beta-adrenergic receptor binding. The effects are rapid and reversible, show a parallel time course, and are blocked by sotalol, a beta-adrenergic receptor antagonist. The beta-mediated regulation of alpha 2-receptor sensitivity at brain norepinephrine synapses may be a mechanism for the homeostatic control of central noradrenergic activity.