gebhart/patrepeval huggingface dataset:
Quadrivalent Vaccine against Human Papillomavirus to Prevent Anogenital Diseases (2007)

The quadrivalent vaccine significantly reduced the incidence of HPV-associated anogenital diseases in young women. (ClinicalTrials.gov number, NCT00092521 [ClinicalTrials.gov].).
Room temperature microwave oscillations in GaN/AlN resonant tunneling diodes with peak current densities up to 220 kA/cm2 (2018)

We report the generation of room temperature microwave oscillations from GaN/AlN resonant tunneling diodes, which exhibit record-high peak current densities. The tunneling heterostructure grown by molecular beam epitaxy on freestanding GaN substrates comprises a thin GaN quantum well embedded between two AlN tunneling barriers. The room temperature current-voltage characteristics exhibit a record-high maximum peak current density of ∼220 kA/cm2. When biased within the negative differential conductance region, microwave oscillations are measured with a fundamental frequency of ∼0.94 GHz, generating an output power of ∼3.0 μW. Both the fundamental frequency and the output power of the oscillator are limited by the external biasing circuit. Using a small-signal equivalent circuit model, the maximum intrinsic frequency of oscillation for these diodes is predicted to be ∼200 GHz. This work represents a significant step towards microwave power generation enabled by resonant tunneling transport, an ultra-fast process that goes beyond the limitations of current III-Nitride high electron mobility transistors.
Stimulation‐dependent lymphokine mRNA levels in human mononuclear cells (1988)

Steady-state mRNA levels for lymphokines were studied in human peripheral blood mononuclear cells stimulated by various agents. The pattern of mRNA levels differed according to the way in which cells were stimulated or activated. Stimulation by anti-CD3 antibodies led to increased mRNA levels for interferon-gamma but only extremely low levels of interleukin 2 mRNA were detected. In order to observe levels of interleukin 2 mRNA, it was necessary to use phorbol myristate acetate (PMA) in addition to either lectins or anti-CD3 antibodies. PMA by itself was already able to induce high levels of mRNA for granulocyte macrophage colony-stimulating factor. Most interestingly, different patterns of lymphokine production were obtained with stimuli yielding the same number of activated cells and the same ratio of activated CD4 and CD8 cells. Thus, different T cell stimuli induced distinct patterns of lymphokine mRNA levels.
Bacterial sepsis in patients with acquired immunodeficiency syndrome (2001)

Departments of Medicine and Medical Microbiology/ Immunology University of Wisconsin Medical School Madison, WI
Evaluation of interference between uplink and downlink in UTRA/TDD (2003)

An ideal TDD system offers high capacity, dynamic asymmetry between uplink and downlink and no coordination requirements between operators and base stations. This paper studies the performance of UTRA/TDD and how close to the ideal TDD system UTRA/TDD could be. Since both uplink and downlink share the same frequency in TDD, those two transmission directions can interfere with each other. This interference can occur between two mobile stations or between two base stations within one carrier or between two operators. This interference scenario is evaluated here. The results show that for UTRA/TDD operation dynamic channel allocation, synchronization of base stations and cooperation between the operators is preferred to obtain the maximum performance. The most difficult interference situations occur between base stations within one operator's band and between two operators if they are not synchronized.
Melanoblasts' Proper Location and Timed Differentiation Depend on Notch/RBP-J Signaling in Postnatal Hair Follicles (2008)

International audience
Phosphodiesterase Type 5 (PDE5) Inhibitors Sensitize Topoisomerase II Inhibitors in Killing Prostate Cancer Through PDE5-Independent Impairment of HR and NHEJ DNA Repair Systems (2019)

Human castration-resistant prostate cancer (CRPC) is a significant target of clinical research. The use of DNA-damaging agents has a long history in cancer chemotherapy but is limited by their toxicities. The combination with a safer drug can be a strategy in reducing dosage and toxicity while increasing anticancer activity in CRPC treatment. Phosphodiesterase type 5 (PDE5) inhibitors are used to treat erectile dysfunction through the selective inhibition of PDE5 that is responsible for cGMP degradation in the corpus cavernosum. Several studies have reported that PDE5 inhibitors display protective effect against doxorubicin-induced cardiotoxicity. The combinatory treatment of CRPC with doxorubicin and PDE5 inhibitors has been studied accordingly. The data demonstrated that sildenafil or vardenafil (two structure-related PDE5 inhibitors) but not tadalafil (structure-unrelated to sildenafil) sensitized doxorubicin-induced apoptosis in CRPC cells with deteriorating the down-regulation of anti-apoptotic Bcl-2 family members, including Bcl-xL and Mcl-1, and amplifying caspase activation. Homologous recombination (HR) and non-homologous end joining (NHEJ) DNA repair systems were inhibited in the apoptotic sensitization through detection of nuclear foci formation of Rad51 and DNA end-binding of Ku80. PDE5 knockdown to mimic the exposure to PDE5 inhibitors did not reproduce apoptotic sensitization, suggesting a PDE5-independent mechanism. Not only doxorubicin, sildenafil combined with other inhibitors of topoisomerase II but not topoisomerase I also triggered apoptotic sensitization. In conclusion, the data suggest that sildenafil and vardenafil induce PDE5-independent apoptotic sensitization to doxorubicin (or other topoisomerase II inhibitors) through impairment of both HR and NHEJ repair systems that are evident by a decrease of nuclear Rad51 levels and their foci formation in the nucleus, and an inhibition of Ku80 DNA end-binding capability. The combinatory treatment may enable an important strategy for anti-CRPC development.
Unconstrained Impedance Control Using a Compact Series Elastic Actuator (2006)

Series elastic actuators offer high fidelity force control with inherent low impedance above their controllable frequency bandwidth. As a result they well suited for interaction with humans, in which safety is paramount. Their high force fidelity suggests that they may be a suitable for impedance control, in which the interaction between motion and force is controlled. The authors model the unconstrained actuator, and perform tests to evaluate the ability of series elastic actuators to mimic different stiffness. Series elastic actuators are found to be adequate for unconstrained impedance control at input frequencies below 3 Hz
Phosphorylation and active ATP hydrolysis are not required for SV40 T antigen hexamer formation. (1993)

ATP induces structural alterations in SV40 large T antigen and promotes changes in its interaction with the viral replication origin. We have analyzed nucleotide-induced changes in T antigen structure in the absence of origin DNA. Most preparations of immunopurified T antigen contain several discrete species ranging in size from monomers through oligomers larger than hexamers. The predominant species consist of monomers and dimers. Incubation of T antigen with ATP or dATP leads to a dramatic and rapid increase in the appearance of T antigen hexamers. Weakly and nonhydrolyzable analogs of ATP are effective as well, indicating that hexamer formation does not require active ATP hydrolysis. After incubation of T antigen with [gamma-35S]ATP, stable association of the labeled nucleotide with all detectable forms occurs. Removal of greater than 80% of the T antigen phosphate residues does not significantly affect the formation of T antigen hexamers, although changes in the distribution and mobility of the other species of T antigen are apparent. Furthermore, T antigen synthesized in and purified from Escherichia coli and, therefore, presumably un- or underphosphorylated, is capable of forming hexamers. Nucleotide-induced T antigen hexamer formation thus appears to require neither protein phosphorylation nor active ATP hydrolysis.
Cytoplasmic RNA in Undifferentiated Neural Stem Cells: A Potential Label-Free Raman Spectral Marker for Assessing the Undifferentiated Status (2012)

Raman microspectroscopy (rms) was used to identify, image, and quantify potential molecular markers for label-free monitoring the differentiation status of live neural stem cells (NSCs) in vitro. Label-free noninvasive techniques for characterization of NCSs in vitro are needed as they can be developed for real-time monitoring of live cells. Principal component analysis (PCA) and linear discriminant analysis (LDA) models based on Raman spectra of undifferentiated NSCs and NSC-derived glial cells enabled discrimination of NSCs with 89.4% sensitivity and 96.4% specificity. The differences between Raman spectra of NSCs and glial cells indicated that the discrimination of the NSCs was based on higher concentration of nucleic acids in NSCs. Spectral images corresponding to Raman bands assigned to nucleic acids for individual NSCs and glial cells were compared with fluorescence staining of cell nuclei and cytoplasm to show that the origin of the spectral differences were related to cytoplasmic RNA. On the basis of calibration models, the concentration of the RNA was quantified and mapped in individual cells at a resolution of ~700 nm. The spectral maps revealed cytoplasmic regions with concentrations of RNA as high as 4 mg/mL for NSCs while the RNA concentration in the cytoplasm of the glial cells was below the detection limit of our instrument (~1 mg/mL). In the light of recent reports describing the importance of the RNAs in stem cell populations, we propose that the observed high concentration of cytoplasmic RNAs in NSCs compared to glial cells is related to the repressed translation of mRNAs, higher concentrations of large noncoding RNAs in the cytoplasm as well as their lower cytoplasm volume. While this study demonstrates the potential of using rms for label-free assessment of live NSCs in vitro, further studies are required to establish the exact origin of the increased contribution of the cytoplasmic RNA.
Metformin Inhibits Growth of Human Glioblastoma Cells and Enhances Therapeutic Response (2015)

High-grade gliomas, glioblastomas (GB), are refractory to conventional treatment combining surgery, chemotherapy, mainly temozolomide, and radiotherapy. This highlights an urgent need to develop novel therapies and increase the efficacy of radio/chemotherapy for these very aggressive and malignant brain tumors. Recently, tumor metabolism became an interesting potential therapeutic target in various cancers. Accordingly, combining drugs targeting cell metabolism with appropriate chemotherapeutic agents or radiotherapy has become attractive. In light of these perspectives, we were particularly interested in the anti-cancer properties of a biguanide molecule used for type 2 diabetes treatment, metformin. In our present work, we demonstrate that metformin decreases mitochondrial-dependent ATP production and oxygen consumption and increases lactate and glycolytic ATP production. We show that metformin induces decreased proliferation, cell cycle arrest, autophagy, apoptosis and cell death in vitro with a concomitant activation of AMPK, Redd1 and inhibition of the mTOR pathway. Cell sensitivity to metformin also depends on the genetic and mutational backgrounds of the different GB cells used in this study, particularly their PTEN status. Interestingly, knockdown of AMPK and Redd1 with siRNA partially, but incompletely, abrogates the induction of apoptosis by metformin suggesting both AMPK/Redd1-dependent and -independent effects. However, the primary determinant of the effect of metformin on cell growth is the genetic and mutational backgrounds of the glioma cells. We further demonstrate that metformin treatment in combination with temozolomide and/or irradiation induces a synergistic anti-tumoral response in glioma cell lines. Xenografts performed in nude mice demonstrate in vivo that metformin delays tumor growth. As current treatments for GB commonly fail to cure, the need for more effective therapeutic options is overwhelming. Based on these results, metformin could represent a potential enhancer of the cytotoxic effects of temozolomide and/or radiotherapy.
The psychology of smart information retrieval (2002)

This article examines the role of user psychology in the development of new approaches to information retrieval. It outlines the broad requirements of any system designed to overcome the problem of information glut, and explores methods for understanding users and eliciting information about their requirements. The importance of systems capturing context is emphasized: both the user’s changing requirements in the context of their underlying preferences, and the context of information contained within documents. The author provides an overview of different technological approaches to classifying information. The article concludes that information retrieval systems can only be truly effective if they construct deep, rich profiles of users as well as understanding the information that they require. Decision Intelligence theory asserts that it is only by bringing together these two halves of the information retrieval equation that users can be matched to content that is truly relevant to their decisionmaking.
International Importation of Measles Virus—United States, 1993–;2001 (2004)

To determine trends in international importations of measles, data from the National Notifiable Diseases Surveillance System were analyzed. Of the 2632 measles cases reported between 1993 and 2001, 449 cases (17%) were internationally imported. An additional 186 cases (7%) resulted from spread of measles virus from these imported cases, and 388 cases (15%) had virological evidence of importation. The number of imported cases averaged 50 per year (range, 26-79 cases). The proportion of cases imported increased from an average of 14% in 1993-1996 to an average of 35% in 1997-2001. Imported measles cases were acquired in 63 countries, with 6 countries (Japan, Germany, China, the Philippines, Italy, and the United Kingdom) accounting for 44% of all imported cases. Further reduction of measles in the United States requires international cooperation and improved global surveillance and control of measles.
Re-evaluating selectivity as a determining factor in peroxidative methane oxidation by multimetallic copper complexes (2015)

A series of multimetallic copper(<sc>ii</sc>) complexes have been re-investigated for methane oxidation with H<sub>2</sub>O<sub>2</sub>.
Supplementary Figure S2 from Tankyrase-Binding Protein TNKS1BP1 Regulates Actin Cytoskeleton Rearrangement and Cancer Cell Invasion (2023)

&lt;p&gt;TNKS1BP1 depletion induces cofilin phosphorylation in HTC75 cells.&lt;/p&gt;
An Inverted Repeat Motif Stabilizes Binding of E2F and Enhances Transcription of the Dihydrofolate Reductase Gene (1995)

An overlapping inverted repeat sequence that binds the eukaryotic transcription factor E2F is 100% conserved near the major transcription start sites in the promoters of three mammalian genes encoding dihydrofolate reductase, and is also found in the promoters of several other important cellular and viral genes. This element, 5'-TTTCGCGCCAAA-3', is comprised of two overlapping, oppositely oriented sites which match the consensus E2F site (5'-TTT(C/G)(C/G)CGC-3'). Recent work has shown that E2F binding activity is composed of at least six related cellular polypeptides which are capable of forming DNA-binding homo- and heterodimers. We have investigated the binding of cellular E2F activity and of homo- and heterodimers of cloned E2F proteins to the inverted repeat E2F element. We have demonstrated that mutations in this element that abolish its inverted repeat nature, while preserving a single consensus E2F site, significantly decrease the binding stability of all of the forms of E2F tested. The rate of association of E2F-1/DP-1 heterodimers with the inverted repeat wild type site was not significantly different from those with the two single site mutated probes. Furthermore, the mutations decrease in vitro transcription and transient reporter gene expression 2-5-fold, an effect equivalent to that of abolishing E2F binding altogether. These data suggest a functional role that may explain the conservation of inverted repeat E2F elements among the DHFR promoters and several other cellular and viral promoters.
Glucose-lowering therapy and cardiovascular outcomes in patients with type 2 diabetes mellitus and acute coronary syndrome (2019)

Diabetes is a common comorbidity in patients hospitalized for an acute coronary syndrome event, and prevalence is increasing. Among patients hospitalized with acute myocardial infarction, diabetes can be an independent predictor of mortality and new cardiovascular events; both short- and long-term outcomes are worse for patients with diabetes relative to those without, and undiagnosed diabetes is associated with greater mortality. The impact of glycemic control on cardiovascular outcomes and the best approach to treat hyperglycemia upon hospital admission for acute coronary syndrome in patients with or without known diabetes remain open questions. This review assesses available evidence for hyperglycemia management at the time of admission for acute coronary syndrome and, thereafter, finds that (1) admission plasma glucose plays a role in predicting adverse events, especially in patients with unknown diabetes; (2) glycated haemoglobin is a likely predictor of events in patients with unknown diabetes; and (3) hypoglycemia at the time of acute myocardial infarction hospital admission is an important predictor for mortality in patients with and without diabetes. Whether glucose-targeted insulin and glucose infusion have advantages over glucose-insulin-potassium infusion remains controversial. Evidence for the effect of novel glucose-lowering agents used at the time of an acute cardiovascular event is limited and requires more dedicated studies.
Adult‐onset cystic fibrosis liver disease: Diagnosis and characterization of an underappreciated entity (2017)

Adult-onset CFLD may be more prevalent than previously described, which suggests a later wave of CFLD that impacts morbidity; routine liver tests, radiologic imaging, noninvasive fibrosis markers, and FibroScan can be used algorithmically to identify adult CFLD; and further evaluation in other CF cohorts should be performed for validation. (Hepatology 2017;66:591-601).
Halogen bonding in UiO-66 frameworks promotes superior chemical warfare agent simulant degradation (2019)

Herein, a series of halogenated UiO-66 derivatives was synthesized and analyzed for the breakdown of the chemical warfare agent simulant dimethyl-4-nitrophenyl phosphate (DMNP) to analyze ligand effects. UiO-66-I degrades DMNP at a rate four times faster than the most active previously reported MOFs. MOF defects were quantified and ruled out as a cause for increased activity. Theoretical calculations suggest the enhanced activity of UiO-66-I originates from halogen bonding of the iodine atom to the phosphoester linkage allowing for more rapid hydrolysis of the P-O bond.
Inter-Plane Satellite Matching in Dense LEO Constellations (2019)

Dense constellations of Low Earth Orbit (LEO) small satellites are envisioned to make extensive use of the inter-satellite link (ISL). Within the same orbital plane, the inter-satellite distances are preserved and the links are rather stable. In contrast, the relative motion between planes makes the inter-plane ISL challenging. In a dense set-up, each spacecraft has several satellites in its coverage volume, but the time duration of each of these links is small and the maximum number of active connections is limited by the hardware. We analyze the matching problem of connecting satellites using the inter-plane ISL for unicast transmissions. We present and evaluate the performance of two solutions to the matching problem with any number of orbital planes and up to two transceivers: a heuristic solution with the aim of minimizing the total cost; and a Markovian solution to maintain the on-going connections as long as possible. The Markovian algorithm reduces the time needed to solve the matching up to 1000x and 10x with respect to the optimal solution and to the heuristic solution, respectively, without compromising the total cost. Our model includes power adaptation and optimizes the network energy consumption as the exemplary cost in the evaluations, but any other QoS-oriented KPI can be used instead.
Dinitrogen Activation Upon Reduction of a Triiron(II) Complex (2014)

Reaction of a trinuclear iron(II) complex, Fe3 Br3 L (1), with KC8 under N2 leads to dinitrogen activation products (2) from which Fe3 (NH)3 L (2-1; L is a cyclophane bridged by three β-diketiminate arms) was characterized by X-ray crystallography. (1) H NMR spectra of the protonolysis product of 2 synthesized under (14) N2 and (15) N2 confirm atmospheric N2 reduction, and ammonia is detected by the indophenol assay (yield ∼30 %). IR and Mössbauer spectroscopy, and elemental analysis on 2 and 2-1 as well as the tri(amido)triiron(II) 3 and tri(methoxo)triiron 4 congeners support our assignment of the reduction product as containing protonated N-atom bridges.
Ionic Liquid Mediated Sol-Gel Synthesis in the Presence of Water or Formic Acid: Which Synthesis for Which Material? (2012)

Sol-gel syntheses involving either neutral water or formic acid as a reactant have been investigated (1) to determine the best conditions to confine a maximum of ionic liquid (IL) inside silica-based matrixes and (2) to reach the highest porosity after removing the IL from the ion gels (washed gels). Several sets of ionogels were prepared from various 1-butyl-3-methylimidazolium ILs and various silica or organosilica sources. The study evidenced a critical effect of the anion on the morphology (monolith, powder) and texture of the resulting washed gels. Particularly, tetrafluoroborate anion led to monolith ionogels by a simple hydrolytic method, affording highly condensed mesoporous silicas with some fluorinated surface sites. Such sites have never been reported before and were evidenced by 19F NMR. On the other hand, formic acid solvolysis turned out to be the only method to get non-exuding, crack-free, and transparent monoliths from ILs containing bis(trifluoromethylsulfonyl)imide [NTf2] anion, with promising applications in photochemistry or photosensing. With bulky imidazolium and pyridinium cations, removal of the IL led to highly porous silicas with pore diameters and pore volumes as high as 10–15 nm and 3 cm3 g–1, respectively. These silicas could find applications as supports for immobilizing bulky molecules.
The Role of MicroRNAs in the Control of Innate Immune Response in Cancer (2014)

Ligands for receptors of natural killer (NK) cells and CD8(+) cytotoxic T lymphocytes (CTL), such as the inhibitory nonclassical HLA-G, the activating stress-induced major histocompatibility complex class I-related antigens MICA and MICB, and/or the UL16-binding proteins (ULBPs), are often aberrantly expressed upon viral infection and neoplastic transformation, thereby preventing virus-infected or malignant-transformed cells from elimination by immune effector cells. Recently, it has been shown that ligands of both NK and CD8(+) T cells are regulated by a number of cellular and/or viral microRNAs (miRs). These miRs are involved in shaping the antiviral and/or antitumoral immune responses as well as neoplastic growth properties. This review summarizes the expression pattern and function of miRs directed against selected NK and T cell receptor ligands, their putative role in shaping immune surveillance and tumorigenicity, and their clinical relevance. In addition, the potential role of RNA-binding proteins in the post-transcriptional gene regulation of these ligands will be discussed.