gebhart/patrepeval huggingface dataset:
DNA detection by THz pumping (2014)

Our results demonstrate a new method for label-free, real-time oligonucleotide characterisation by their self-resonant modes, which are unique to their conformation and sequence. We anticipate that our assay will be used as a starting point for a more detailed investigation of the aforementioned mechanism, which can be used as a basis for oligonucleotide detection and analysis. Furthermore, this technique can be applied to improve existing modern genetics technologies.
Experimental woodpile EBG waveguides, bends and power dividers at microwave frequencies (2006)

Experimental waveguides, bends and power dividers in the woodpile electromagnetic bandgap (EBG) material at Ku-band are demonstrated. Prototypes are fabricated from alumina, and use an efficient waveguide transition to enable high quality measurements. Low-loss transmission is demonstrated for a waveguide and a 90° bend, and near equal power division shown for a woodpile EBG waveguide power divider. The components have the potential to be scaled for applications at millimetre-wave and terahertz frequencies.
Abstract 73: Aberrant Activation of γ2-AMPK Increases Cardiac Growth Through Cellular Hypertrophy and Hyperplasia (2012)

AMP-activated protein kinase (AMPK) is an energy sensor and a key regulator of cell metabolism, hence a promising drug target. Point mutations in the regulatory γ2-subunit (encoded by PRKAG2 gene) have been shown to cause a unique form of cardiomyopathy in humans characterized by cardiac growth, arrhythmias and glycogen storage. In previous studies, we demonstrated that the mutation of prkag2 (N488I) caused aberrant activation of AMPK leading to glycogen storage. However, elimination of glycogen storage by inhibiting glycogen synthase activity failed to normalize heart weight (HW) of the mutant mice. Here, we aimed to determine whether cardiac growth in PRKAG2 ardiomyopathy was dueto cellular hypertrophy or hyperplasia. We used transgenic mice expressing a mutant PRKAG2 (N488I) in the heart (TGγ2 N488I ) that faithfully recapitulated PRKAG2 cardiomyopathy. We determined HW and cardiac myocyte size in adult (2 months) and postnatal (2 weeks) hearts in WT and TGγ2 N488I . At 2 months, TGγ2 N488I hearts show a 2.4-fold increase in HW/BW (body weight) (10.3 ± 1.44 vs. 4.3± 0.17 mg/g) as well as cross-sectional cell surface area compared WT hearts (325 ± 13 vs. 155 ± 5.4 μm 2 ,p&lt;0.01), suggesting cellular hypertrophy in adult TGγ2 N488I heart. Furthermore, we observed increased mTOR activity evidenced by enhanced phosphorylation of mTOR (Ser2448) as well as its downstream targets S6 and 4E-BP. The HW of TGγ2 N488I was partially inhibited by treatment with rapamycin, an inhibitor of mTOR. Interestingly, the length and width of isolated cardiomyocytes from 2 weeks old mice were not different between the WT and TGγ2 N488I heart in spite of a 50% increase of HW of TGγ2 N488I mice. We observed a 2- fold increase in the expression of a proliferation marker, proliferating cell nuclear antigen (PCNA) during postnatal cardiac growth. Expression of Cyclin genes including cyclin D1, D2 and E1 was greatly increased in TGγ2 N488I hearts (2.4 - 4 fold, p&lt;0.01 vs. WT). Taken together, these data indicate that aberrant γ2-AMPK activation stimulates cardiac growth through increased cell number during postnatal growth period and increased cell size at adulthood. These results suggest a novel role of γ2-AMPK in the growth of cardiac myocytes.
ChemInform Abstract: ARTIFICIAL CARBOHYDRATE ANTIGENS: SYNTHESIS OF RHAMNOSE TRISACCHARIDE AND DISACCHARIDE HAPTENS COMMON TO SHIGELLA FLEXNERI O‐ANTIGENS (1980)

Abstract Die Synthese des Disaccharids (IVb) und die des Trisaccharids (VIb) und deren Hydrazide (IVc) und (VIc) werden beschrieben.
Racial differences in running and landing measures associated with injury risk vary by sex (2022)

It is unknown whether running and landing mechanics differ between racial groups despite injury disparities between African Americans (AA) and white Americans (WA). This study aimed to identify potential racial differences in running and landing mechanics and understand whether anthropometric, strength, and health status factors contribute to these differences. Venous blood samples, anthropometry, lower-extremity strength, and health status assessments were collected (n = 84, 18-30y). Three-dimensional motion capture and force plate data were recorded during 7 running and 7 drop vertical jump trials. Racial effects were determined, and regression models evaluated explanatory factors. AA females ran with longer stance times (p = 0.003) than WA females, while AA males ran with smaller loading rates (p = 0.046) and larger peak vertical ground reaction forces (p = 0.036) than WA males. Frontal plane knee range of motion during landing was greater in AA females (p = 0.033) than WA females; larger waist circumference and weaker knee extension strength accounted for this significance. Although outcome measures were associated with physiologic, anthropometric, and activity measures, their explanatory power for race was ambiguous, except for knee range of motion in females. Modifiable factors explaining racial effects during landing in females are potential intervention targets to reduce racial health disparities in running and landing injuries.
M-T5, the Ankyrin Repeat, Host Range Protein of Myxoma Virus, Activates Akt and Can Be Functionally Replaced by Cellular PIKE-A (2006)

The myxoma virus (MV) ankyrin repeat, host range factor M-T5 has the ability to bind and activate cellular Akt, leading to permissive MV replication in a variety of diverse human cancer cell lines (G. Wang, J. W. Barrett, M. Stanford, S. J. Werden, J. B. Johnston, X. Gao, M. Sun, J. Q. Cheng, and G. McFadden, Proc. Natl. Acad. Sci. USA 103:4640-4645, 2006). The susceptibility of permissive human cancer cells to MV infection is directly correlated with the basal or induced levels of phosphorylated Akt. When M-T5 is deleted from MV, the knockout virus, vMyxT5KO, can no longer productively infect a subset of human cancer cells (designated type II) that exhibit little or no endogenous phosphorylated Akt. In searching for a host counterpart of M-T5, we noted sequence similarity of M-T5 to a recently identified ankyrin repeat cellular binding protein of Akt called PIKE-A. PIKE-A binds and activates the kinase activity of Akt in a GTP-dependent manner and promotes the invasiveness of human cancer cell lines. Here, we demonstrate that transfected PIKE-A is able to rescue the ability of vMyxT5KO to productively infect type II human cancer cells that were previously resistant to infection. Also, cancer cells that were completely nonpermissive for both wild-type and vMyxT5KO infection (called type III) were rendered fully permissive following ectopic expression of PIKE-A. We conclude that the MV M-T5 host range protein is functionally interchangeable with the host PIKE-A protein and that the activation of host Akt by either M-T5 or PIKE-A is critical for the permissiveness of human cancer cells for MV.
CONVENIO DE BECAS CON EL INSTITUTO DE NUTRICIÓN (2014)

LA FACULTAD DE CIENCIAS SIGNO UN
CONVENIO DE COLABORACION CON EL
INSTITUTO NACIONAL DE CIENCIAS
MEDICAS Y NUTRICION SALVADOR
ZUBIRAN, CON EL QUE SE BENEFICIA A LA COMUNIDAD
UNIVERSITARIA MEDIANTE BECAS
PARA ESTUDIANTES DE LICENCIATURA, MAESTRIA
Y DOCTORADO.
Development of a Stable Episomal Shuttle Vector for Toxoplasma gondii (1998)

The rapid developments in the molecular genetics of Toxoplasma gondii have far reaching implications in treatment and vaccination strategies for this as well as closely related pathogens such as Plasmodium. Although stable transformation of this parasite through homologous and illegitimate genomic integration has provided many of the tools necessary for genetic analysis, subsequent manipulations of the DNA have proven laborious. This report describes the selection and subsequent characterization of a Toxoplasma sequence that permits the episomal maintenance of bacterial plasmids in this parasite. This sequence was isolated from the Toxoplasma genome through selection for episomal stability of a pUC19-based library in the absence of a selectable marker. A 500-base pair fragment was determined to possess the stabilization activity. Transformations of Toxoplasma using vectors possessing this fragment, referred to as EMS (episomal maintenance sequence), demonstrated an elevated stable transformation frequency compared with the vector alone. Mutants deficient in hypoxanthine-xanthine-guanine phosphoribosyltransferase activity were used as a test to see if this gene could be selected from a genomic library using a vector containing the EMS. The success of this test demonstrates the utility of EMS-containing vectors in complementation strategies and the ability of such constructs bearing large fragments of the Toxoplasma genome to be maintained episomally.
Complementary DNA Cloning of HSC71, a 71 kDa Heat Shock Cognate Protein, in Japanese Flounder &lt;i&gt;Paralichtys olivaceus&lt;/i&gt; (1998)

Full-length cDNA for a 71 kDa constitutively expressed heat-shock-cognate protein (HSC71), a member of the HSP70 family, was isolated from a Japanese flounder embryo cDNA library. It has a single ORF of 1950bp that encodes a protein of 71. 2 kDa. Japanese flounder HSC71 contains a EEVD (Glu-Glu-Val-Asp) peptide motif at the C-terminal end which is a common feature of cytosolic HSC proteins in the HSP70 family. Japanese flounder HSC71 is 96.0%, 93.9%, 93.5%, and 93.4% identical in primary structure to rainbow trout HSC71, bovine HSC71, rat HSC71, and human HSC71, respectively. These results suggest that the HSC71 amino acid sequence has been highly conserved among vertebrates. Northern blot analysis showed that HSC71 mRNA was expressed constitutively in the Japanese flounder cells in early passage regardless of heat shock treatment.
&lt;title&gt;Flat panel head-up display&lt;/title&gt; (2000)

The dominance of the Cathode Ray Tube (CRT) in the specialist area of Head Up Displays (HUDs) is being challenged by Flat Panel Displays (FPDs). The luminance and contrast requirements are very demanding, a problem compounded by the low efficiency of the display devices. This paper discusses the application of transmissive or reflective Liquid Crystal Display (LCD) to the HUD and particularly reviews the illumination of the display. The conclusion is that in the near term a transmissive configuration with an RF coupled or Light Emitting Diode (LED) backlight shows most promise but that longer term micro-lasers allied with the reflective configuration should be a better solution.
Automated analysis method to assess pulmonary blood flow distribution using conventional X-ray angiography (2022)

Quantitative assessment of the right-to-left ratio of pulmonary blood flow distribution is important for determining the clinical indications for treating pulmonary arterial branch stenosis. A novel theory was recently proposed that can be used to quantitatively assess the right-to-left ratio on conventional X-ray angiography images. In the proposal, further developments were indicated, especially automated calculation. In this study, a new automated algorithm was developed. In the X-ray image, regions of interest were set in right and left lung, and time-signal intensity curves were measured. The new automated algorithm is applied to determine the optimal time window for the analysis of the time-signal intensity curve and to calculate the slope of the curve in the optimized time window. The right-to-left ratios in seven consecutive patients calculated by the new automated algorithm were compared to those calculated by lung perfusion scintigraphy. The ratios were in good agreement with linear regression with a slope of 1.27 and a Pearson correlation coefficient of 0.95. The processing time was less than 10 s, which is one-eighth of the manual processing time. The new automated algorithm is accurate, stable, and fast enough for clinical use in the real world.
Regulation of Brome Mosaic Virus Gene Expression by Restriction of Initiation of Protein Synthesis (1980)

The translation of total and individual brome mosaic virus (BMV) RNAs was examined in a wheat germ cell-free system in the presence of various inhibitors. Inhibitors of the initiation of polypeptide synthesis, e.g., potassium ions, 7-methylguanosine 5' -monophosphate, and aurintricarboxylic acid, were shown not only to inhibit overall BMV protein synthesis but also to change the ratio of BMV polypeptides synthesized. Under conditions restrictive for initiation, the translation of nonstructural BMV genes was suppressed, but coat protein synthesis proceeded at a high rate. A similar discrimination among BMV messengers was exerted by a regulatory protein kinase isolated from wheat germ. These results suggest that the regulation of the expression of BMV genes is based on a difference in the mechanism of formation of initiation complexes for individual BMV messages.
Mechanical behavior of the human lumbar and lumbosacral spine as shown by three-dimensional load-displacement curves. (1994)

The lumbar region is a frequent site of spinal disorders, including low-back pain, and of spinal trauma. Clinical studies have established that abnormal intervertebral motions occur in some patients who have low-back pain. A knowledge of normal spinal movements, with all of the inherent complexities, is needed as a baseline. The present study documents the complete three-dimensional elastic physical properties of each lumbar intervertebral level from the level between the first and second lumbar vertebrae through the level between the fifth lumbar and first sacral vertebrae. Nine whole fresh-frozen human cadaveric lumbar-spine specimens were used. Pure moments of flexion-extension, bilateral axial torque, and bilateral lateral bending were applied, and three-dimensional intervertebral motions were determined with use of stereophotogrammetry. The motions were presented in the form of a set of six load-displacement curves, quantitating intervertebral rotations and translations. The curves were found to be non-linear, and the motions were coupled. The ranges of motion were found to compare favorably with reported values from in vivo studies.
ChemInform Abstract: Large‐Scale Preparation of Polyfunctional Benzylic Zinc Reagents by Direct Insertion of Zinc Dust into Benzylic Chlorides in the Presence of Lithium Chloride. (2010)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Resilient hollow fiber nanofiltration membranes fabricated from crosslinkable phase-separated copolymers (2020)

Hollow fiber nanofiltration membranes are fabricated from a copolymer designed to withstand exposure to solvents and chlorine.
Learning reconfigurable scene representation by tangram model (2012)

This paper proposes a method to learn reconfigurable and sparse scene representation in the joint space of spatial configuration and appearance in a principled way. We call it the tangram model, which has three properties: (1) Unlike fixed structure of the spatial pyramid widely used in the literature, we propose a compositional shape dictionary organized in an And-Or directed acyclic graph (AOG) to quantize the space of spatial configurations. (2) The shape primitives (called tans) in the dictionary can be described by using any “off-the-shelf” appearance features according to different tasks. (3) A dynamic programming (DP) algorithm is utilized to learn the globally optimal parse tree in the joint space of spatial configuration and appearance. We demonstrate the tangram model in both a generative learning formulation and a discriminative matching kernel. In experiments, we show that the tangram model is capable of capturing meaningful spatial configurations as well as appearance for various scene categories, and achieves state-of-the-art classification performance on the LSP 15-class scene dataset and the MIT 67-class indoor scene dataset.
Jagged1 (JAG1) mutations in patients with tetralogy of fallot or pulmonic stenosis (2010)

Mutations in the Notch pathway ligand Jagged1 (JAG1) cause Alagille syndrome (AGS), as well as cardiac defects in seemingly nonsyndromic individuals. To estimate the frequency of JAG1 mutations in cases with right-sided cardiac defects not otherwise diagnosed with AGS, we screened 94 cases with tetralogy of Fallot (TOF) and 50 with pulmonic stenosis/peripheral pulmonary stenosis (PS/PPS) or pulmonary valve atresia with intact ventricular septum (PA) for mutations. Sequence changes were identified in three TOF and three PS/PPS/PA patients, that were not present in 100 controls. We identified one frameshift and two missense mutations in the TOF cases, and one frameshift and two missense mutations in cases with PS/PPS/PA. The four missense mutations were assayed for their effect on protein localization, posttranslational modification, and ability to activate Notch signaling. The missense mutants displayed heterogeneous behavior in these assays, some with complete haploinsufficiency, suggesting that there are additional modifiers leading to organ specific features. We identified functionally significant mutations in 2% (2/94) of TOF patients and 4% (2/50) of PS/PPS/PA patients. Patients with right-sided cardiac defects should be carefully screened for features of AGS or a family history of cardiac defects that might suggest the presence of a JAG1 mutation.
Design of a visible-light spectroscopy clinical tissue oximeter (2005)

We develop a clinical visible-light spectroscopy (VLS) tissue oximeter. Unlike currently approved near-infrared spectroscopy (NIRS) or pulse oximetry (SpO2%), VLS relies on locally absorbed, shallow-penetrating visible light (475 to 625 nm) for the monitoring of microvascular hemoglobin oxygen saturation (StO2%), allowing incorporation into therapeutic catheters and probes. A range of probes is developed, including noncontact wands, invasive catheters, and penetrating needles with injection ports. Data are collected from: 1. probes, standards, and reference solutions to optimize each component; 2. ex vivo hemoglobin solutions analyzed for StO2% and pO2 during deoxygenation; and 3. human subject skin and mucosal tissue surfaces. Results show that differential VLS allows extraction of features and minimization of scattering effects, in vitro VLS oximetry reproduces the expected sigmoid hemoglobin binding curve, and in vivo VLS spectroscopy of human tissue allows for real-time monitoring (e.g., gastrointestinal mucosal saturation 69+/-4%, n=804; gastrointestinal tumor saturation 45+/-23%, n=14; and p<0.0001), with reproducible values and small standard deviations (SDs) in normal tissues. FDA approved VLS systems began shipping earlier this year. We conclude that VLS is suitable for the real-time collection of spectroscopic and oximetric data from human tissues, and that a VLS oximeter has application to the monitoring of localized subsurface hemoglobin oxygen saturation in the microvascular tissue spaces of human subjects.
Multi‐Scale Finite‐Volume Method for Elliptic Problems with Heterogeneous Coefficients and Source Terms (2006)

Abstract Simulation of sub‐surface flow in geologically complex formations is just one example in computational science, where efficient and accurate solutions of heterogeneous elliptic problems are of great interest. Often it is not feasible to resolve the whole range of relevant length scales associated with the spatial distribution of the highly varying coefficients. The MSFV method was originally developed for multi‐phase flow in porous media. In the more general form presented here, it can be applied for solving large elliptic problems in various areas of computational science. (© 2006 WILEY‐VCH Verlag GmbH &amp; Co. KGaA, Weinheim)
Effect of Dietary Calcium Supplements and Amlodipine on Growth, Arterial Blood Pressure, and Cardiac Hypertrophy of Spontaneously Hypertensive Rats (2003)

It has been shown that calcium-enriched diets cause a decrease in arterial blood pressure in hypertensive patients and animals. Moreover, it has been suggested that the combination of dietary calcium and calcium antagonists could paradoxically have a synergistic effect in order to decrease arterial blood pressure. The combination of these treatments may also have potential therapeutic benefit in the attenuation of cardiac hypertrophy. In this study, after being weaned at three weeks, male spontaneously hypertensive rats (SHR) were randomized in four groups of animals. Two of these groups were fed on a normal calcium diet (Ca 1%) and another two groups were fed on a calcium-enriched diet (Ca 2.5%). One of the groups fed on each diet also received amlodipine (1 mg/kg/day) in the drinking water after being weaned. Body weight was measured weekly in all the groups, and arterial blood pressure was also measured in all the 10-, 15-, 20-, and 25-week-old SHR by the tail cuff method. We established the ratio heart weight/body weight x 1000 (R), and we weighed the left ventricle in the 25-week-old animals at the end of the different treatments. The Ca 2.5% diet caused a delay in the development of hypertension in SHR. This effect could not be correlated with alterations in body weight since this diet improved growth in these rats. Amlodipine did not alter growth in SHR. This pharmacological treatment caused a decrease in the arterial blood pressure of the SHR and it intensified the antihypertensive effect of the Ca 2.5% diet. Nevertheless, this drug attenuated the effect of dietary calcium on body weight and when the treatment was prolonged, almodipine also antagonized the effect of dietary calcium on arterial blood pressure. At the end of the different treatments the ratio R and the left ventricular weight were similar in all the groups of animals. Therefore, the use of dietary calcium with a calcium antagonist to control arterial blood pressure seems not to be advisable, and the present data do not prove the usefulness of the mentioned antihypertensive treatments in preventing cardiac hypertrophy.
Continuous Flow Sodiation of Substituted Acrylonitriles, Alkenyl Sulfides and Acrylates (2020)

The sodiation of substituted acrylonitriles and alkenyl sulfides in a continuous flow set-up using NaDA (sodium diisopropylamide) in EtNMe<sub>2</sub> or NaTMP (sodium 2,2,6,6-tetramethylpiperidide)⋅TMEDA in n-hexane provides sodiated acrylonitriles and alkenyl sulfides, which are subsequently trapped in batch with various electrophiles such as aldehydes, ketones, disulfides and allylic bromides affording functionalized acrylonitriles and alkenyl sulfides. This flow-procedure was successfully extended to other acrylates by using Barbier-type conditions.
Disentangling the roles of point-of-sale ban, tobacco retailer density and proximity on cessation and relapse among a cohort of smokers: findings from ITC Canada Survey (2018)

Banning POS retail displays shows promise as an additional tool to prevent relapse, although these results need to be confirmed in larger longitudinal studies.