gebhart/patrepeval huggingface dataset:
Angiotensin-(1-7) Promotes Resolution of Neutrophilic Inflammation in a Model of Antigen-Induced Arthritis in Mice (2017)

Defective resolution of inflammation may be crucial for the initiation and development of chronic inflammatory diseases, such as arthritis. Therefore, it has been suggested that therapeutic strategies based on molecules that facilitate inflammation resolution present great potential for the treatment of chronic inflammatory diseases. In this study, we investigated the effects and role of angiotensin-(1-7) [Ang-(1-7)] in driving resolution of neutrophilic inflammation in a model of arthritis. For this purpose, male C57BL/6 mice were subjected to antigen-induced arthritis and treated with Ang-(1-7) at the peak of the inflammatory process. Analysis of the number of inflammatory cells, apoptosis, and immunofluorescence for NF-κB was performed in the exudate collected from the knee cavity. Neutrophil accumulation in periarticular tissue was measured by assaying myeloperoxidase activity. Apoptosis of human neutrophil after treatment with Ang-(1-7) was evaluated morphologically and by flow cytometry, and NF-κB phosphorylation by immunofluorescence. Efferocytosis was evaluated <i>in vivo</i>. Therapeutic treatment with Ang-(1-7) at the peak of inflammation promoted resolution, an effect associated with caspase-dependent neutrophils apoptosis and NF-κB inhibition. Importantly, Ang-(1-7) was also able to induce apoptosis of human neutrophils, an effect associated with NF-κB inhibition. The pro-resolving effects of Ang-(1-7) were inhibited by the Mas receptor antagonist A779. Finally, we showed that Ang-(1-7) increased the efferocytic ability of murine macrophages. Our results clearly demonstrate that Ang-(1-7) resolves neutrophilic inflammation <i>in vivo</i> acting in two key step of resolution: apoptosis of neutrophils and their removal by efferocytosis. Ang-(1-7) is a novel mediator of resolution of inflammation.
A cDNA probe (PheA12) from the hc-(ERBA) gene on chromosome 3 detects a high frequency RFLP (1988)

Journal Article A cDNA probe (PheA12) from the hc-(ERBA) gene on chromosome 3 detects a high frequency RFLP Get access A.E. Bale, A.E. Bale National Institutes of HealthBethesda, MD 20892, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar S. Usala, S. Usala National Institutes of HealthBethesda, MD 20892, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar C. Weinberger, C. Weinberger National Institutes of HealthBethesda, MD 20892, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar B.D. Weintraub, B.D. Weintraub National Institutes of HealthBethesda, MD 20892, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar O.W. McBride O.W. McBride National Institutes of HealthBethesda, MD 20892, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 16, Issue 15, 11 August 1988, Page 7756, https://doi.org/10.1093/nar/16.15.7756 Published: 11 August 1988
Elements of Programming Interviews: The Insiders' Guide (2012)

This is a larger-format version of Elements of Programming Interviews. The language is C++. Specifically, the font size is larger, and the page size is 7"x10" (the regular format uses 6"x9"). The content is identical. Have you ever... Wanted to work at an exciting futuristic company? Struggled with an interview problem that could have been solved in 15 minutes? Wished you could study real-world computing problems? If so, you need to read Elements of Programming Interviews (EPI). EPI is your comprehensive guide to interviewing for software development roles. The core of EPI is a collection of over 250 problems with detailed solutions. The problems are representative of interview questions asked at leading software companies. The problems are illustrated with 200 figures, 300 tested programs, and 150 additional variants. The book begins with a summary of the nontechnical aspects of interviewing, such as strategies for a great interview, common mistakes, perspectives from the other side of the table, tips on negotiating the best offer, and a guide to the best ways to use EPI. We also provide a summary of data structures, algorithms, and problem solving patterns. Coding problems are presented through a series of chapters on basic and advanced data structures, searching, sorting, algorithm design principles, and concurrency. Each chapter stars with a brief introduction, a case study, top tips, and a review of the most important library methods. This is followed by a broad and thought-provoking set of problems. A practical, fun approach to computer science fundamentals, as seen through the lens of common programming interview questions.
Polyoxometalate Photocatalysis for Liquid-Phase Selective Organic Functional Group Transformations (2018)

Polyoxometalates (POMs), which are anionic metal oxide clusters, have recently attracted considerable attention as photocatalysts because of their unique photoinduced charge-transfer properties, redox properties, acid–base properties, and reactivities. In this Review, we present a summary of recent developments in POM photocatalysis for organic synthesis. Various organic functional group transformations can be selectively induced by photoirradiation in the presence of catalytic amounts of suitably designed POMs. In particular, many liquid-phase functional group transformations based on the activation of substrates by decatungstate have been reported. However, decatungstate photocatalysis requires irradiation with UV light because of the large energy gaps between the O2–-based highest occupied molecular orbitals (HOMOs) and the W6+-based lowest unoccupied molecular orbitals (LUMOs) therein. Various strategies have been developed in efforts to utilize visible light, including hybridization with photosensitizers, metal substitution, and coordination of ligands (substrates) at the vacant sites of lacunary POMs. We also present here an overview of our recent work on the development of visible-light-responsive POM catalysts by HOMO- and LUMO-engineering strategies for both the oxidation and reduction of organic substrates, including amines, alcohols, nitroarenes, sulfides, sulfoxides, and pyridine N-oxides.
Data from A 26-Gene Hypoxia Signature Predicts Benefit from Hypoxia-Modifying Therapy in Laryngeal Cancer but Not Bladder Cancer (2023)

&lt;div&gt;Abstract&lt;p&gt;&lt;b&gt;Purpose:&lt;/b&gt; Tumor hypoxia is associated with a poor prognosis, hypoxia modification improves outcome, and hypoxic status predicts benefit from treatment. Yet, there is no universal measure of clinical hypoxia. The aim of this study was to investigate whether a 26-gene hypoxia signature predicted benefit from hypoxia-modifying treatment in both cancer types.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Experimental Design:&lt;/b&gt; Samples were available from 157 T2–T4 laryngeal cancer and 185 T1–T4a bladder cancer patients enrolled on the accelerated radiotherapy with carbogen and nicotinamide (ARCON) and bladder carbogen nicotinamide (BCON) phase III randomized trials of radiotherapy alone or with carbogen and nicotinamide (CON) respectively. Customized TaqMan low density arrays (TLDA) were used to assess expression of the 26-gene signature using quantitative real-time PCR. The median expression of the 26 genes was used to derive a hypoxia score (HS). Patients were categorized as TLDA-HS low (≤median) or TLDA-HS high (&gt;median). The primary outcome measures were regional control (RC; ARCON) and overall survival (BCON).&lt;/p&gt;&lt;p&gt;&lt;b&gt;Results:&lt;/b&gt; Laryngeal tumors categorized as TLDA-HS high showed greater benefit from ARCON than TLDA-HS low tumors. Five-year RC was 81% (radiotherapy alone) versus 100% (CON) for TLDA-HS high (&lt;i&gt;P&lt;/i&gt; = 0.009). For TLDA-HS low, 5-year RC was 91% (radiotherapy alone) versus 90% (CON; &lt;i&gt;P&lt;/i&gt; = 0.90). TLDA-HS did not predict benefit from CON in bladder cancer.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Conclusion:&lt;/b&gt; The 26-gene hypoxia signature predicts benefit from hypoxia-modifying treatment in laryngeal cancer. These findings will be evaluated in a prospective clinical trial. &lt;i&gt;Clin Cancer Res; 19(17); 4879–88. ©2013 AACR&lt;/i&gt;.&lt;/p&gt;&lt;/div&gt;
In situ Management of Renal Tumors: Renal Cell Carcinoma and Transitional Cell Carcinoma (1984)

In the last 8 years 15 patients with malignant neoplasms in functionally solitary kidneys underwent in situ excision of the tumor with preservation of renal parenchyma. Of 10 patients with renal cell carcinoma 8 underwent partial nephrectomy, 1 had a central wedge resection and 1 had enucleation of 3 tumors. After followup of 6 months to 4.7 years 6 patients are free of disease. One patient died of metastatic cancer and 1 of cardiovascular disease. Of 5 patients with transitional cell carcinoma of the kidney (including 2 with parenchymal invasion) 2 underwent partial nephrectomy and 3 underwent extensive resection of renal pelvic lesions. After followup of 6 months to 7 years 2 patients are alive, 2 died of metastatic disease and 1 died of metastatic bladder carcinoma. Only 1 of the 5 patients had locally recurrent tumor. These data demonstrate the efficacy of in situ management of renal tumors in selected patients with solitary kidneys or compromised renal function.
Bladder Cancer Associated Glycoprotein Signatures Revealed by Urinary Proteomic Profiling (2007)

Current methods in the noninvasive detection and surveillance of bladder cancer via urine analysis include voided urine cytology (VUC) and some diagnostic urinary protein biomarkers; however, due to the poor sensitivity of VUC and high false-positive rates of currently available protein assays, detection of bladder cancer via urinalysis remains a challenge. In the study presented here, a rapid, high-sensitivity technique was developed to profile the N-linked glycoprotein component in naturally micturated human urine specimens. Concanavalin A (Con A) affinity chromatography coupled to nanoflow liquid chromatography was utilized to separate the complex peptide mixture prior to a linear ion trap MS analysis. Of 186 proteins identified with high confidence by multiple analyses, 40% were secreted proteins, 18% membrane proteins, and 14% extracellular proteins. In this study, the presence of several proteins appeared to be associated with the presence of bladder cancer, including alpha-1B-glycoprotein that was detected in all tumor-bearing patient samples but in none of the samples obtained from non-tumor-bearing individuals. The combination of Con A affinity chromatography and nano-LC/MS/MS provides an initial investigation of N-glycoproteins in complex biological samples and facilitates the identification of potential biomarkers of bladder cancer in noninvasively obtained human urine.
Preparative Fluorous Mixture Synthesis of Diazonium-Functionalized Oligo(phenylene vinylene)s (2005)

[structure: see text] A series of building blocks for the synthesis of oligo(phenylene vinylene)s (OPVs) and hybrid oligomers were prepared, and alternating Heck coupling and Horner-Wadswoth-Emmons (HWE) reactions were used to couple the building blocks. Model studies were carried out to optimize the reaction strategies. The products were made to bear aryl diazonium functionalities that allow them to be used as surface grafting moieties in hybrid silicon/molecule assemblies. A library of OPV and hybrid oligomer tetramers was synthesized using fluorous mixture synthesis (FMS). The fluorous tags, which are secondary amines bearing different numbers of fluorine atoms, were synthesized and used as phase tags in mixture synthesis. The tags and substrates were anchored together by triazene linkages. The mixture synthesis was monitored by analytical HPLC on a fluorous column, and isolation of final OPV and hybrid oligomer tetramers was achieved by preparative HPLC. At the end of the FMS, after demixing, the tagged products were detagged by cleaving the triazene linkage and generating a series of aryl diazonium compounds. The fluorous tags could be recovered and reused. The NMR spectra of the 1-aryl-3,3-dialkyltriazenes are discussed.
[Nanotechnology-based regenerative medicine--cell sheet engineering utilizing temperature-responsive culture dishes]. (2006)

Regenerative medicine is believed to overcome shortcomings of conventional therapies including symptomatic drug therapy and surgical removal. We have utilized nanotechnology for regenerative medicine to develop temperature-responsive culture dishes, on which a temperature-responsive polymer is covalently grafted with precise control of its density and thickness in a nanometer scale. Confluent cells cultured on the dishes are intactly harvested as a single contiguous cell sheet by reducing temperature below 32 degrees C, and harvested cell sheets are subjected to tissue reconstruction, named cell sheet engineering. Here, we introduce the examples of clinical applications including corneal regeneration as well as large animal studies.
A CASE OF CECAL VOLVULUS DIAGNOSED PREOPERATIVELY BY MULTIPLANAR REFORMATION AND CT-COLONOGRAPHY (2006)

MDCTによるmultiplanar reformation(以下MPR)像とCT-colonographyにて術前診断した盲腸軸捻転症の1例を経験した.症例は45歳,女性.便秘症にて下剤常用し, 2度イレウスにて保存的治療を受けた.腹痛にて受診し,腹部単純X線像にて鏡面像を有するガス像を認めイレウスの診断で入院した.腹部CTで,拡張を伴わない上行結腸の右側から腹側に著明に拡張する腸管を認めた.腸管の走行を明らかにするため作成した. CT-colonographyではループ状に拡張した回盲部,その両端にbird's beak sign, その口側に拡張した回腸を認めた.また, MPRの冠状断像では下腹部にwhirl-like appearanceを認めた.以上より盲腸軸捻転症と診断し手術を施行した,盲腸は反時計回りに180度捻転したうえに左上腹部に向かって180度屈曲していた.この屈曲および捻転を整復した後,再発予防のため盲腸を後腹膜に固定した.術後問題なく退院,現在まで再発は認めていない.
GENE EXPRESSION ANALYSIS OF A DEDIFFERENTIATED LIPOSARCOMA — DIFFERENCES BETWEEN HIGH AND LOW GRADE AREAS: ANALYSIS OF TWO CASES AND LITERATURE REVIEW (2005)

The phenomenon of dedifferentiation typically occurs in soft tissue sarcomas where a low grade or well-differentiated tumor shows an abrupt transformation to a high-grade sarcoma without lineage specificity. The biological behavior and metastatic potential of these tumors is dictated by the dedifferentiated phenotype. Tumor material was available from two dedifferentiated liposarcomas. We performed cDNA microarray analysis of a dedifferentiated liposarcoma in which the atypical lipomatous/well-differentiated and dedifferentiated portions were grossly distinct, to find differentially expressed genes in the dedifferentiated component compared to the well-differentiated component. There were 100 differentially expressed genes, both up- and down-regulated in the high grade sarcoma. In addition, we performed RT-PCR on selected genes in both cases to confirm the microarray findings. We discuss the expression patterns of these genes in comparison to other studies in the literature.
Supplementary Table 1 from CEACAM1-3S Drives Melanoma Cells into NK Cell-Mediated Cytolysis and Enhances Patient Survival (2023)

&lt;p&gt;Supplementary Table 1. Quantitative expression of CEACAM1 splice variants in melanoma cell lines.&lt;/p&gt;
Human β2‐adrenergic receptor/GSα fusion protein, expressed in 2 ras‐dependent murine carcinoma cell lines, prevents tumor growth in syngeneic mice (1997)

We report a strategy of tumor growth inhibition based on the expression of a foreign protein with both potential anti-proliferative and immunogenic properties. To validate our approach, we used 2 ras-mutated murine carcinoma cell lines (carB and C57/PDV) transfected with the gene encoding a fusion protein containing the human β2-adrenergic receptor and the α subunit of the Gs protein (β2Gs). We previously showed that the sustained activation of the β2Gs fusion protein expressed in carB cells (carB β2Gs cells) induced a cAMP-dependent inhibition of cell growth in vitro. Here, we observed inhibition of tumor growth after s.c. inoculation of 2 carB β2Gs clones (10C2 and 20F4) in syngeneic ICFW mice. We thus selected 3 C57/PDV β2Gs clones (2D3, 5F3 and 1G1) in which activation of the fusion protein was not efficiently coupled to the cAMP-PKA signaling pathway. Contrasting with carB β2Gs clones, activation of the fusion protein in these C57/PDV β2Gs clones did not have any anti-proliferative effect in vitro. Therefore, they were good candidates to assess the immunogenic property of the fusion protein. Accordingly, none of the C57/PDV β2Gs clones formed tumors in immunocompetent syngeneic C57BL/6 mice, while they were still tumorigenic in nude mice. Most interestingly, all of the β2Gs clones that did not form tumors, from both cell lines, provided protection against respective wild-type tumor development. Our results show that expression of the β2Gs fusion protein in cancer cells elicits inhibition of cell proliferation and/or immune rejection of both β2Gs-modified and wild-type tumor cells. Int. J. Cancer 71: 1029-1034, 1997. © 1997 Wiley-Liss Inc.
ChemInform Abstract: Synthesis and Nematocidal Activities of Arylvinyltetrahydropyrimidines. (1994)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Dynamic response of an array of flexural plates in acoustic medium (2012)

The dynamic response of a transducer array made up of circular flexural plates in immersion is analytically calculated. The calculation method includes three steps: (1) the calculation of parallel resonant frequency and the velocity profile of each plate, (2) the calculation of mutual acoustic impedance between the plates, and (3) the calculation of velocity response, including the mechanical and acoustic impedance. The calculation method is validated by both finite element analysis and measurement results of a fabricated capacitive micromachined ultrasonic transducer. Based on the calculated velocity, the near-field pressure and the near-to-far field radiation patterns are presented. The flexural plate array in immersion displays two modes of operation. At low frequency, the mode shape of the transducer array is similar to that of a suspended plate and, at certain frequencies, two groups of plates move in opposite phase, which results in the cancellation of the average velocity. At high frequency, the mode shape is similar to that of a piston transducer; however, the near-field pressure distribution is similar to that of a resilient disk.
Hydrolytic degradation of polyisobutylene and poly-L-lactide-based multiblock copolymers Part A Polymer chemistry (2010)

The hydrolytic degradation of a series of poly-L-lactide (PLLA)-polyisobutylene (PIB) multiblock copolymers was studied in phosphate buffer solution (pH = 7.4) at 37 °C. The multiblock copolymers were synthesized by chain extension of PLLA-b-PIB-b-PLLA triblock copolymers, which were obtained by ring-opening polymerization of L-lactide initiated by hydroxyallyl telechelic PIB. The degradation strongly depended on the PLLA segment length. At constant PIB segment length, the multiblock copolymer with the shortest PLLA segment length (DPn = 10), showed significant weight loss after 8 weeks, whereas weight loss for DPn = 36 was only observed after 24 weeks. The gel-permeation chromatographic analysis showed a similar decrease in the number-average molecular weight (Mn) with time further supporting the weight loss data. Dynamic mechanical analysis showed a decrease in ultimate stress and modulus with time. The crystallinity of multiblock copolymers changed significantly with degradation time as indicated from differential scanning calorimetric analysis.
The anti-tumor effect of shikonin on osteosarcoma by inducing RIP1 and RIP3 dependent necroptosis (2013)

Shikonin had prompt but profound anti-tumor effect on both primary and metastatic osteosarcoma, probably by inducing RIP1 and RIP3 dependent necroptosis. Shikonin would be a potential anti-tumor agent on the treatment of primary and metastatic osteosarcoma.
Serum Stimulation of the c-<i>fos</i> Enhancer Induces Reversible Changes in c-<i>fos</i> Chromatin Structure (1990)

Transcription of the proto-oncogene c-fos is known to be activated by growth factors in serum and subsequently repressed by the Fos protein. We show that generalized DNase I sensitivity of c-fos chromatin correlates closely with enhancer activity during induction, repression, and superinduction of the c-fos gene. Within 90 s of serum stimulation, proximal DNA sequences on both sides of the enhancer exhibit increased DNase I sensitivity. Within 5 min, elevated DNase I sensitivity spreads to chromatin at the distal 3' end of the c-fos gene. These results suggest that an open state of chromatin is propagated in both directions from the enhancer. The induced alterations in chromatin structure precede the increased transcriptional activity of the c-fos gene, suggesting that these changes in chromatin structure potentiate transcription.
Expression of Cell Adhesion Molecules in Endometrial Tissue and Endometrioid Adenocarcinomas (2021)

Endometrioid endometrial adenocarcinomas are the most common histological variant of malignant tumors in the uterine cavity. In turn, the features of expression by neoplastic cells of intercellular adhesion molecules are a reliable prognostic factor in the progression of malignant tumors. One of the important indicators of cancer progression is E-cadherin, which determines the strength of intercellular adhesion and the ability of cells to spread. Among other adhesion molecules, considerable attention has recently been paid to the molecules of cell adhesion of carcino-embryonic antigen 1 (MCA-REA1). Therefore, the purpose of the study was to study the expression of E-cadherin and MCA-REA1 in normal endometrium and endometrioid adenocarcinomas. Materials and methods. To achieve this purpose, we performed tissue studies of 10 samples of normal endometrium and 30 samples of endometrioid endometrial adenocarcinoma (8380/3). Morphological features of neoplastic tissue were studied by hematoxylin and eosin staining. Visualization of E-cadherin and MCA-REA1 receptors was determined using the appropriate antibodies and the UltraVision Quanto Detection System HRP DAB Chromogen (Thermo scientific, USA) in similar areas of the tumor on serial sections. Results and discussion. It has been shown that endometrial tissue demonstrates different expression of MCA-REA1 and E-cadherin receptors in the normal state and in endometrioid adenocarcinomas. This indicates the absence of any functional correlation between them. Expression of MCA-REA1 was detected on the apical surface of the luminal and glandular columnar epithelium. In contrast, the endometrioid endometrial carcinoma tissues showed the pronounced heterogeneous location of MCA-REA1 in tumor cells. Moreover, due to the tumor dedifferentiation, these proteins disappear from the cell surface. On the other hand, E-cadherin is normally localized in intercellular contacts and epithelial-mesenchymal junctions. During carcinoma dedifferentiation, the intensity of E-cadherin expression decreased, which was accompanied by an increase in nuclear polymorphism of cancer cells and focal separation of cells from the total tumor mass. Conclusion. The variability of the expression patterns of MCA-REA1 and E-cadherin in the dedifferentiation of endometrioid adenocarcinoma may be an indicator of neoplastic transformation and progression of the malignant process