gebhart/patrepeval huggingface dataset:
Flow Injection Amperometric Detection at Enzyme‐Modified Gold Nanoelectrodes (2004)

Abstract Gold nanotubular electrode ensembles were prepared by using electroless deposition of the metal within the pores of polycarbonate particle track‐etched membranes. Glucose oxidase (Gox), used as a model enzyme, has been immobilized onto preformed self‐assembled monolayers (mercaptoethylamine or mercaptopropionic acid) on electroless gold via cross‐linking with glutaraldehyde or covalent attachment by carbodiimide coupling. Flow‐injection analysis systems in flow‐through or wall‐jet configurations using these Gox‐modified nanoelectrodes are described. The influence of different experimental parameters (i.e., applied potential, flow rate, interferents…︁) on the analytical response of the sensor to glucose has been evaluated. Under optimized conditions, very reproducible results (standard deviations &lt;4%, n =38) were obtained, linear calibration was achieved in the 2×10 −4 M to 3×10 −2 M concentration range and the detection limit was 2×10 −4 M. Moreover, no significant interferences from species like ascorbic and uric acids were observed at a potential of +0.9 V.
Grid Scale Energy Storage: Modeling of Electrolyzer-Fuel Cell Combination and Comparison with Flow Batteries (2020)

Life cycle cost modeling of hydrogen production via Polymer Electrolyte Membrane (PEM) and Solid Oxide (SO) electrolysis and discharge via fuel cell combination is investigated for large grid scale baseload storage applications. The cost of hydrogen in $/kg and storage energy produced in $/kWh are compared for four cases. The high-end simulations assume parameters that are capable within the next five years and low-end cases represent post significant improvements in the cost and durability of the technologies in the next couple of decades. The levelized cost of gclose to commercialization scale (low-end) for PEM and SO electrolysis is calculated to be ~$1.6 per kg. Levelized cost of energy (LCOE) calculations with this cost of hydrogen coupled with fuel cells is ~$0.10 per kWh. These values compare favorably to future flow battery potential where an LCOE of ~$0.09 per kWh is possible.
Regional expression of genes mediating trans-synaptic alpha-synuclein transfer predicts regional atrophy in Parkinson disease (2018)

Multiple genes have been implicated in Parkinson disease pathogenesis, but the relationship between regional expression of these genes and regional dysfunction across the brain is unknown. We address this question by joint analysis of high resolution magnetic resonance imaging data from the Parkinson's Progression Markers Initiative and regional genetic microarray expression data from the Allen Brain Atlas. Regional brain atrophy and genetic expression was co-registered to a common 86 region brain atlas and robust multivariable regression analysis was performed to identify genetic predictors of regional brain atrophy. Top candidate genes from GWAS analysis, as well as genes implicated in trans-synaptic alpha-synuclein transfer and autosomal recessive PD were included in our analysis. We identify three genes with expression patterns that are highly significant predictors of regional brain atrophy. The two most significant predictors are LAG3 and RAB5A, genes implicated in trans-synaptic synuclein transfer. Other well-validated PD-related genes do not have expression patterns that predict regional atrophy, suggesting that they may serve other roles such as disease initiation factors.
Development of low density silica aerogel as a capture medium for hyper-velocity particles (1989)

The objective of this study is to develop micro-porous silica aerogel glass with optimal properties for its potential use as a capture medium of hyper-velocity particles. The important properties for this application are identified as ultra-low bulk density (i.e. < 0.1 gm/cc), high transparency to visible light, and purity (absence of organic and inorganic contaminants). Our approach is to expand on our existing knowledge and experience concerning sol-gel chemistry and the extraction technology which we now use to routinely produce clear silica aerogel monoliths at densities higher than 0.1 gm/cc. We expect to push the existing technology toward ultra-low density aerogels and identify any limitations impeding the development of this material with optimal properties for the particle capture application. 3 refs., 10 figs.
Decay of the hydroperoxyl spin adduct of 5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide: an EPR kinetic study (1995)

The decay kinetics of the hydroperoxyl spin adduct of both 5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide (DEPMPO), a new β-phosphorylated cyclic nitrone and 5,5-dimethyl-1-pyrroline N-oxide (DMPO) were studied in various media by EPR spectroscopy. In organic solvents, both first- and second-order processes were shown to contribute to the decay of the two spin adducts. However, in aqueous solution the DMPO–hydroperoxyl spin adduct decay was pure first-order. The half-lives of the two spin adducts were determined in every medium tested and the DEPMPO–hydroperoxyl spin adduct was shown to be significantly more persistent (from five times in organic solvents to 30 times in a pH 5.6 buffer) than the DMPO–hydroperoxyl spin adduct.
NiO/Poly(4-alkylthiazole) Hybrid Interface for Promoting Spatial Charge Separation in Photoelectrochemical Water Reduction (2020)

Conjugated polymers are emerging as alternatives to inorganic semiconductors for the photoelectrochemical water splitting. Herein, semi-transparent poly(4-alkylthiazole) layers with different trialkylsilyloxymethyl (R<sub>3</sub>SiOCH<sub>2</sub>-) side chains (PTzTNB, R = <i>n</i>-butyl; PTzTHX, R = <i>n</i>-hexyl) are applied to functionalize NiO thin films to build hybrid photocathodes. The hybrid interface allows for the effective spatial separation of the photoexcited carriers. Specifically, the PTzTHX-deposited composite photocathode increases the photocurrent density 6- and 2-fold at 0 V versus the reversible hydrogen electrode in comparison to the pristine NiO and PTzTHX photocathodes, respectively. This is also reflected in the substantial anodic shift of onset potential under simulated Air Mass 1.5 Global illumination, owing to the prolonged lifetime, augmented density, and alleviated recombination of photogenerated electrons. Additionally, coupling the inorganic and organic components also enhances the photoabsorption and amends the stability of the photocathode-driven system. This work demonstrates the feasibility of poly(4-alkylthiazole)s as an effective alternative to known inorganic semiconductor materials. We highlight the interface alignment for polymer-based photoelectrodes.
Concomitant Prevalence of Low Serum Diamine Oxidase Activity and Carbohydrate Malabsorption (2016)

The aim of this retrospective study was to analyze the concomitant prevalence rates for lactose malabsorption (LM), fructose malabsorption (FM), and histamine intolerance (HI) in patients with so far unexplained gastrointestinal (GI) symptoms. A total of 439 outpatients, who presented unclear abdominal discomfort, underwent lactose (50 g) and fructose (25 g) hydrogen (H<sub>2</sub>) breath tests. Additionally, serum diamine oxidase (DAO) measurements were performed. Individuals with low serum DAO activity (<10 U/mL), GI symptoms, and response to histamine-free diet were diagnosed with HI. Of all 439 patients, 341 (77.7%) were found with 7 various GI conditions. In total, 94 (21.4%), 31 (7.1%), and 100 (22.8%) individuals presented LM, FM, or HI only, whereas 116 (26.4%) patients showed an overlap of GI entities investigated here. Interestingly, 89 out of 241 (36.9%) individuals with carbohydrate malabsorption were also diagnosed with HI (LM + HI: 52 [11.8%], FM + HI: 23 [5.2%], and LM + FM + HI 14 [3.2%] individuals). In conclusion different combinations of LM, FM, and HI are present in individuals with unclear abdominal discomfort/pain. In clinical practice we suggest testing for LM, FM, and additional HI in the diagnostic work-up of these patients. Depending on these various diagnoses possible, patients should get an individualized dietary advice.
Isolation and Molecular Weight Characterization of Tetragonula laeviceps Honey Protein (2018)

The concentration of protein in honey is very low compare with polysaccharide. However, recently the honey’s protein is intensively studied and it shown that protein also have several biological activities such as antibacterial activity. The purpose of this research is to isolate and characterize Tetragonula laeviceps honey protein by determining the molecular weight. Honey protein of Trigona laeviceps was isolated using filtration with N2 pressure (membrane size 30 kda) then concentrated using Millipore TM tube membrane size 10 kDa. Molecular weight was analyzed by SDS PAGE. From analysis, there was major protein band in honey produced by Tetragona laeviceps identified. The produced molecular weight of major protein band was 87 kDa. Determining of the molecular weight of this protein could be used to detect the originality of Tetragonula laeviceps honey from Indonesia.
Heat Shock Protein 60 as a Mediator of Adipose Tissue Inflammation and Insulin Resistance (2012)

The stress protein heat shock protein 60 (Hsp60) induces secretion of proinflammatory mediators from murine adipocytes. This study aimed to study Hsp60 as a mediator of adipose tissue inflammation and skeletal muscle cell (SkMC) insulin sensitivity and to quantify plasma Hsp60 concentrations in lean and obese individuals. Regulation of Hsp60 release and Hsp60-induced cytokine secretion and signaling was measured in human adipocytes and SkMCs. Adipocytes exhibited higher Hsp60 release than preadipocytes and SkMCs, which was further stimulated by cytokines and Toll-like receptor (TLR)-4 activation. Hsp60 activated extracellular signal-related kinase (ERK)-1/2, Jun NH(2)-terminal kinase (JNK), p38, nuclear factor (NF)-κB, and impaired insulin-stimulated Akt phosphorylation in adipocytes. Furthermore, Hsp60 stimulated adipocytes to secrete tumor necrosis factor-α, interleukin (IL)-6, and IL-8. In SkMCs, Hsp60 activated ERK1/2, JNK, and NF-κB and inhibits insulin signaling and insulin-stimulated glucose uptake. SkMCs released IL-6, IL-8, and monocyte chemoattractant protein-1 on Hsp60 stimulation. Plasma Hsp60 was higher in obese males than in lean males and correlated positively with BMI, blood pressure, leptin, and homeostasis model assessment-insulin resistance. In summary, Hsp60 is released by human adipocytes, increased in plasma of obese humans, and induces insulin resistance. This is accompanied by activation of proinflammatory signaling in human adipocytes and SkMCs. Thus, Hsp60 might be a factor underlying adipose tissue inflammation and obesity-associated metabolic disorders.
Obstacles to Implementing Evidence‐Based Dentistry: A Focus Group‐Based Study (2008)

In many countries, questions have been raised about the use of evidence-based practice (EBP) in oral health care. The call for an increase in EBP seems to face many obstacles. Only limited empirical studies address these obstacles. We present a qualitative study that explores the obstacles that Flemish (Belgian, Dutch-speaking) dentists experience in the implementation of EBP in routine clinical work. We collected data from discussions in focus groups. Seventy-nine dentists participated. The data were analyzed using constant comparative analysis. Three major categories of obstacles were identified. These categories relate to obstacles in 1) evidence, 2) partners in health care (medical doctors, patients, and government), and 3) the field of dentistry. Our findings suggest that educators should provide communication skills to aid decision making, address the technical dimensions of dentistry, promote lifelong learning, and close the gap between academics and general practitioners (dentists) in order to create mutual understanding. The obstacles identified are considered useful to support future quantitative research that can be generalized to a broader group.
7-DAY, IN PARALLEL ARTIFICIAL LUNG TESTING IN SHEEP (2005)

An implantable artificial lung, the MC3 Biolung®, was attached to the pulmonary circulation of four male sheep (32.1±3.6kg) in parallel with the natural lungs for 7 days. The purpose of the study was to determine the long-term effects of attachment on device performance and animal physiology and hematology. After the surgery, the animals were awakened from anesthesia and breathed room air thereafter. Heart rate (HR), mean arterial pressure (Pa), central venous pressure (Pcvp) were monitored continuously, arterial blood gases were obtained every 1 to 4 hours, and platelet counts were taken once a day. A flow probe was placed on the TAL inlet tubing to measure the TAL flow (QTAL). After 7 days, the animals were euthanized and necropsied. Hemodynamics remained stable over 7 days in all animals, and the averages were HR = 136± 6.8 beats/min, Pa = 98.1± 3.2 mmHg, Pcvp = 8.1±1.9 mmHg, respectively. Arterial blood gases were maintained in good condition as PO2 = 114±10 mmHg, PCO2 = 37.7±1.6 mmHg. The TAL function was also maintained well, average oxygen transfer rate was 113±15 mL/min. There was no infection in the chest or evidence of embolism in vital organs at necropsy. A few small clots were observed on the TAL housing but the TAL inlet and outlet were clean after 7 days. These results indicate that the TAL is suitable for 7 day attachment.
Morphology and Three-Dimensional Inhalation Flow in Human Airways in Healthy and Diseased Subjects (2017)

University of Minnesota M.S. thesis.September 2017. Major: Aerospace Engineering and Mechanics. Advisor: Filippo Coletti. 1 computer file (PDF); vi, 53 pages.
<i>In Situ</i> Follow-Up of the Intercalation Process in a Clay/Polymer Nanocomposite Model System by Rheo-XRD Analyses (2009)

This work deals with the follow-up of structure evolution in clay−polymer nanocomposites during the intercalation process through in situ analyses combining X-ray diffraction and rheology using a home-modified X-ray diffraction setup coupled with a rheometer with a special shearing cell developed for this work. To avoid any interference of medium elasticity, the selected matrix was a bisphenol A-based epoxy resin DGEBA with a Newtonian behavior under the examined experimental conditions. The selected clay was an organophilic montmorillonite (Cloisite 30B). The evolution of clay stacks in the clay−polymer nanocomposites was assessed under quiescent conditions by simultaneous time-resolved X-ray diffraction (XRD) and rheometry. The main results showed a very slow polymer diffusion process that increases the concentration of the intercalated stacks, narrows their distribution, and decreases the number of lamellae per stack.
Size sorting of chemically modified graphene nanoplatelets (2013)

Size-sorted graphene nanoplatelets are highly desired for fundamental research and technological applications of graphene. Here we show a facile approach for fabricating size-sorted graphene oxide (GO) nanoplatelets by a simple centrifugal method using different dispersion solvents. We found that the small-sized GO nanoplatelets were more effectively separated when dispersed in water or dimethylformamide (DMF) than in an alkali aqueous solution. After several iterations of the centrifugation, the sizes of GO in the supernatant solution were mostly several micrometers. We found that the GO area was not strongly correlated with the C-O content of the GO dispersed in water. However, the size-sorted GO nanoplatelets in DMF showed different C-O content, since DMF can reduce GO nanoplatelets during exfoliation and centrifugation processes.
Scalability Aspects of Cell-Free Massive MIMO (2019)

Ubiquitous cell-free massive MIMO (multiple-input multiple-output) combines massive MIMO technology and user-centric transmission in a distributed architecture. All the access points (APs) in the network cooperate to jointly and coherently serve a smaller number of users in the same time-frequency resource. However, this coordination needs significant amounts of control signalling which introduces additional overhead, while data co-processing increases the back/front-haul requirements. Hence, the notion that the “whole world” could constitute one network, and that all APs would act as a single base station, is not scalable. In this study, we address some system scalability aspects of cell-free massive MIMO that have been neglected in literature until now. In particular, we propose and evaluate a solution related to data processing, network topology and power control. Results indicate that our proposed framework achieves full scalability at the cost of a modest performance loss compared to the canonical form of cell-free massive MIMO.
Guidelines for the Acute Medical Management of Severe Traumatic Brain Injury in Infants, Children, and Adolescents-Second Edition Author Affiliations (2012)

Author Affiliations Patrick M. Kochanek, MD, FCCM, Professor and Vice Chair, Department of Critical Care Medicine, University of Pittsburgh School of Medicine Nancy Carney, PhD, Associate Professor, Department of Medical Informatics and Clinical Epidemiology, Oregon Health & Science University P. David Adelson, MD, FACS, FAAP, Director, Barrow Neurological Institute at Phoenix Children’s Hospital, Chief, Pediatric Neurosurgery/ Children’s Neurosciences Stephen Ashwal, MD, Distinguished Professor of Pediatrics and Neurology, Chief of the Division of Child Neurology, Department of Pediatrics, Loma Linda University School of Medicine Michael J. Bell, MD, Associate Professor of Critical Care Medicine, University of Pittsburgh School of Medicine Susan Bratton, MD, MPH, FAAP, Professor of Pediatric Critical Care Medicine, University of Utah School of Medicine Susan Carson, MPH, Senior Research Associate, Department of Medical Informatics and Clinical Epidemiology, Oregon Health & Science University Randall M. Chesnut, MD, FCCM, FACS, Professor of Neurological Surgery, Orthopedics and Sports Medicine, University of Washington School of Medicine Jamshid Ghajar, MD, PhD, FACS, Clinical Professor of Neurological Surgery, Weill Cornell Medical College, President of the Brain Trauma Foundation Brahm Goldstein, MD, FAAP, FCCM, Senior Medical Director, Clinical Research, Ikaria, Inc., Professor of Pediatrics, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School Gerald A. Grant, MD, Associate Professor of Surgery and Pediatrics, Duke University School of Medicine Niranjan Kissoon, MD, FAAP, FCCM, Professor of Paediatrics and Emergency Medicine, British Columbia’s Children’s Hospital, University of British Columbia Kimberly Peterson, BSc, Research Associate, Department of Medical Informatics and Clinical Epidemiology, Oregon Health & Science University Nathan R. Selden, MD, PhD, FACS, FAAP, Campagna Professor and Vice Chair of Neurological Surgery, Oregon Health & Science University Robert C. Tasker, MBBS, MD, FRCP, Chair and Director, Neurocritical Care, Children’s Hospital Boston, Professor of Neurology and Anesthesia, Harvard Medical School Karen A. Tong, MD, Associate Professor of Radiology, Loma Linda University Monica S. Vavilala, MD, Professor of Anesthesiology and Pediatrics, University of Washington School of Medicine Mark S. Wainwright, MD, PhD, Director, Pediatric Neurocritical Care, Associate Professor of Pediatrics, Northwestern University Feinberg School of Medicine Craig R. Warden, MD, MPH, MS, Professor of Emergency Medicine and Pediatrics, Chief, Pediatric Emergency Services, Oregon Health & Science University/Doernbecher Children’s Hospital
Large difference but high correlation between creatinine and cystatin C estimated glomerular filtration rate in Mesoamerican sugarcane cutters (2022)

Creatinine increases among heat-stressed workers reflect reduced glomerular filtration as estimated using eGFR<sub>cys</sub>, a marker independent of muscle mass and metabolism. The discrepancy between eGFR<sub>cr</sub> and eGFR<sub>cys</sub> may indicate reduced glomerular filtration of larger molecules and/or systemic bias in CKD-EPI performance in this population.
Vascular Adhesion Protein-1 Enhances Tumor Growth by Supporting Recruitment of Gr-1(+)CD11b(+) Myeloid Cells into Tumors (2009)

Cancer growth is regulated by several nonmalignant cell types, such as leukocytes and endothelial cells, which reside in the stroma of the tumor. Vascular adhesion protein-1 (VAP-1)is an amine oxidase enzyme that is expressed on the surface of endothelial cells. It supports leukocyte traffic into inflamed tissues, but nothing is known about its possible role in cancer biology in vivo. Here, we report that B16 melanoma and EL-4 lymphoma remain smaller in VAP-1–deficient mice than in wild-type controls. We found an unexpected defect in tumor angiogenesis in the absence of VAP-1. VAP-1 also selectively enhanced the recruitment of Gr-1 + CD11b + myeloid cells into the tumors. Generation of mice expressing enzymatically inactive VAP-1 showed that the oxidase activity of VAP-1 was necessary to support neoangiogenesis, myeloid cell recruitment, and tumor growth in vivo. These data describe VAP-1 as the first adhesion molecule known to be involved in the recruitment of Gr-1 + CD11b + myeloid cells into tumors. They also suggest that VAP-1 is a potential new tool for immunotherapy of tumors that could be exploited to reduce tumor burden by controlling the traffic of Gr-1 + CD11b + myeloid cells. [Cancer Res 2009;69(19):7875–83]
Toward Individualized Therapy in Acute Myeloid Leukemia (2015)

Acute myeloid leukemia (AML) is a heterogeneous disease in its clinical presentation, response to therapy, and overall prognosis. For decades, pretreatment karyotype evaluation has served to identify subgroups for risk-adapted postremission therapy, but the initial treatment approach has been largely unchanged. With continued advances in the genetic and epigenetic characterization of AML, we have discovered even more diversity and are starting to understand the biological underpinnings of these multiple disease entities. Newer therapies are being developed to address the pathophysiology within these individual AML subsets. This review categorizes AML into biologically defined groups based on currently available data and describes the evolving treatment approaches within these groups. Identifying the genetic abnormalities and biological drivers prior to AML treatment will be important as we work to individualize therapy and improve outcomes.
Nicotine response genetics in the zebrafish (2009)

Tobacco use is predicted to result in over 1 billion deaths worldwide by the end of the 21(st) century. How genetic variation contributes to the observed differential predisposition in the human population to drug dependence is unknown. The zebrafish (Danio rerio) is an emerging vertebrate model system for understanding the genetics of behavior. We developed a nicotine behavioral assay in zebrafish and applied it in a forward genetic screen using gene-breaking transposon mutagenesis. We used this method to molecularly characterize bdav/cct8 and hbog/gabbr1.2 as mutations with altered nicotine response. Each have a single human ortholog, identifying two points for potential scientific, diagnostic, and drug development for nicotine biology and cessation therapeutics. We show this insertional method generates mutant alleles that are reversible through Cre-mediated recombination, representing a conditional mutation system for the zebrafish. The combination of this reporter-tagged insertional mutagen approach and zebrafish provides a powerful platform for a rich array of questions amenable to genetic-based scientific inquiry, including the basis of behavior, epigenetics, plasticity, stress, memory, and learning.