gebhart/patrepeval huggingface dataset:
Abstract 5394: Uterine leiomyosarcoma with homologous recombination deficiency is highly sensitive to polo-like kinase 4 inhibitor (2022)

Abstract INTRODUCTION: Uterine leiomyosarcoma (LMS) is rare and aggressive and without a well-established cancer-driver. Treatment is mainly surgical because most other therapies have limited benefits. Polo-like kinase 4 (PLK4) is a modulator of centriole duplication that is essential for proper cell division. Its inhibition by the PLK4 inhibitor, CFI-400945 resulted in centriole dysregulation, increased in double-strand DNA damage, and ultimately, mitotic catastrophe and cell death. Recent genomic studies have identified a subset of LMS with homologous recombination (HR) repair deficiency. We hypothesize that HR-deficient LMS is highly sensitive to CFI-400945. METHODS: Gene knockouts (KO) were done by CRISPR-Cas9 system. Plasmids encoding the BRCA1- and BRCA2-targeting guide RNA, Cas9 protein, and selection markers were generated and were transfected into SK-UT-1 and SKN followed by selection with antibiotics. The KO and wild-type cell lines were treated with CFI-400945 in vitro. Percentage apoptosis was evaluated by Annexin V-PI staining and cell proliferation was examined by sulforhodamine B assay. To determine whether BRCA1- and BRCA2-KO had any effects on double-strand DNA damage, the γH2AX foci were studied by immunofluorescence staining. RESULTS: CFI-400945 caused a greater degree of apoptosis and less cell viability in the KO than in the wild-type cell lines. In SK-UT-1, quantity of apoptotic cells for BRCA2-KO and BRCA1-KO were 77% and 34%, respectively, versus 27% in the wild-type cell line (p&amp;lt;0.05). Less dramatic findings were observed in SKN with respective percentages of 20% and 25% versus 15% (p&amp;lt;0.05). In SK-UT-1, the mean IC50 for BRCA2-KO and BRCA1-KO were 3.4 nM and 5.5 nM, respectively, versus 7.8 nM in the wild-type cell line (p&amp;lt;0.05). Similar observations were noted in SKN, with respective mean IC50 of 6.5 nM and 7.1 nM versus 10.0 nM (p&amp;lt;0.05). After CFI-400945 treatment, the γH2AX/Hoechst 33342 staining intensity ratio was 0.40 in SK-UT-1 BRAC2-KO and 0.44 in BRCA1-KO, where both were higher than in the corresponding wild-type cell line with 0.29 (p&amp;lt;0.05). Similarly, the intensities in the two SKN KO cell lines were 0.29 and 0.31, respectively, and higher than in the wild-type cell line with 0.19 (p &amp;lt;0.05). CONCLUSIONS: PLK4 inhibitor induced DNA double-strand breaks in LMS and the repair of these breakages was dependent on HR repair. In vitro, LMS with BRCA1 or BRCA2 deficiencies were more sensitive to the effect of PLK4 inhibitor. Citation Format: Tsz Yan Chong, Horace HY Lee, Judy WP Yam, Kin Long Chow, Ho Shing Wong, Ka Yu Tse, Mark R. Bray, Tak Wah Mak, Philip PC Ip. Uterine leiomyosarcoma with homologous recombination deficiency is highly sensitive to polo-like kinase 4 inhibitor [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 5394.
Vocal Cord Control Methods based on Neural Network Forward and Inverse Models for Anthropomorphic Talking Robot (2008)

We have developed a vocal control method based on forward and inverse model, and new vocal cord mechanism, for anthropomorphic talking robot, Waseda Talker No.7, to produce various kinds of voices. The previous artificial vocal cord model could reproduce human-like vibration, however, the control range of the acoustic parameters are very small. We developed new mechanism could adjust the vocal cords tension effectively and control the glottal opening-closing, to broaden the control range. The control parameters of the vocal cords on WT-7 are vocal cord tension, glottal opening and air pressure, and the acoustic parameters are sound pitch, spectrum tilt and sound pressure. The relations between these parameters are complicated, and hard to modeling. The learning process consists of learning of the NN forward model and learning of the inverse model by using the forward model. In addition, the real-time auditory feedback mechanism is used to reduce the error. By this method, the acoustic parameters could be followed well the target.
Plasma deposition of piezoelectric ZnO layers by rf sputtering, SolGel and pulsed laser deposition (2008)

Abstract As “lab‐on‐a‐chip‐devices” suited for analyses of least amounts of liquids are emerging from prototype status, cost‐effective materials for mass production of these devices are sought. For handling and mixing components, surface acoustic waves generated by piezoelectric elements are routinely employed; however, the LiNbO 3 single crystals used in such units are a significant cost factor. As an alternative, zinc oxide layers deposited onto the glass substrates hold the promise of cheaper production and easier integration into the assembly. In the present study, experiments regarding the deposition of such layers using different plasma processes are presented. Film synthesis was performed using rf magnetron sputtering, pulsed laser deposition and plasma based ion bombardment of Sol‐Gel films on crystalline and amorphous substrates. The impacts of significant deposition parameters are discussed. At optimum deposition parameters, excellent columnar growth in the preferred c‐axis orientation could be observed. The suitability of such films for the desired application is substanciated through first mixing experiments using optically lithographed interdigital transducers (IDTs). (© 2008 WILEY‐VCH Verlag GmbH &amp; Co. KGaA, Weinheim)
Effective Prophylaxis of COVID-19 in Rhesus Macaques Using a Combination of Two Parenterally-Administered SARS-CoV-2 Neutralizing Antibodies (2021)

SARS-CoV-2 is a respiratory borne pathogenic beta coronavirus that is the source of a worldwide pandemic and the cause of multiple pathologies in man. The rhesus macaque model of COVID-19 was utilized to test the added benefit of combinatory parenteral administration of two high-affinity anti-SARS-CoV-2 monoclonal antibodies (mAbs; C144-LS and C135-LS) expressly developed to neutralize the virus and modified to extend their pharmacokinetics. After completion of kinetics study of mAbs in the primate, combination treatment was administered prophylactically to mucosal viral challenge. Results showed near complete virus neutralization evidenced by no measurable titer in mucosal tissue swabs, muting of cytokine/chemokine response, and lack of any discernable pathologic sequalae. Blocking infection was a dose-related effect, cohorts receiving lower doses (6, 2 mg/kg) resulted in low grade viral infection in various mucosal sites compared to that of a fully protective dose (20 mg/kg). A subset of animals within this cohort whose infectious challenge was delayed 75 days later after mAb administration were still protected from disease. Results indicate this combination mAb effectively blocks development of COVID-19 in the rhesus disease model and accelerates the prospect of clinical studies with this effective antibody combination.
396 Endoscopic Submucosal Dissection for Resection of T1b N0 Esophageal Cancer: Retrospective Multicenter Study (2019)

INTRODUCTION: While esophagectomy is the standard treatment for submucosal (T1b) esophageal adenocarcinoma (EAC) and squamous cell cancer (SCC), many elderly patients -the age group that esophageal cancer tends to affect- are not candidates for surgery. Endoscopic submucosal dissection (ESD) is a minimally invasive procedure that allows for en-bloc resection of early esophageal cancer and allows for precise histopathologic staging of the tumor. ESD could be curative in submucosal cancer without lymph node metastasis, and it allows for precise histopathologic staging. To evaluate the long-term outcomes of ESD in patients with EUS-staged T1b esophageal cancer. METHODS: A retrospective multicenter study was performed at three academic hospitals in the U.S. Patients who underwent ESD with a pre-procedural or post-procedural histopathologic diagnosis of T1b N0 M0 EAC or SCC were included in the study. Data was collected on demographics, tumor and procedure characteristics, procedure pathology, and follow-up. Local recurrence was confirmed by endoscopic findings and pathological analysis. Metastasis was confirmed by cross-sectional imaging. The main outcome was a composite of rate of metastasis and local recurrence. Follow-up time was defined as months from initial procedure to recurrence or last follow-up visit if recurrence-free. A time-to-event analysis was performed to evaluate recurrence. RESULTS: Fifty-two patients underwent ESD (mean age 69.6 years +/-9; 35 males) (Table 1). Based on available comorbidity data, 42% patients were not fit for surgery, as determined by an age-adjusted Charlson Comorbidity Index &gt; 6. Twenty tumors (62%) were found to be HGD or T1a on post-ESD specimen analysis, while one was staged as T2. Nineteen patients were staged as T1b after ESD. Immediately after ESD, 6 patients were referred for surgery, 3 underwent chemoradiation therapy, and 6 radiation alone (Table 2). Patients were followed for a median of 18 months, and the calculated 24-month recurrence rate was 13% (Figure 1). CONCLUSION: This multicenter trial showed that ESD for T1b esophageal cancer can be acceptable for patients who deemed not surgical candidates. However, close follow up is necessary. ESD can also serve as a staging procedure, as our data confirms inaccurate EUS-staging of T1b cancer, with 62% of tumors being down-staged to T1a, as has been previously reported.
Trends in Active Cigarette Smoking Among Stroke Survivors in the United States, 1999 to 2018 (2020)

Background and Purpose- Patients who continue to smoke after a stroke face a higher risk of recurrent stroke. While several effective drugs for smoking cessation became available over the past 2 decades, whether active smoking has decreased among stroke survivors is unknown. We, therefore, evaluated trends in active smoking among stroke survivors during this period. Methods- We performed trends analyses using cross-sectional data collected every 1 to 2 years from 2 US health surveys spanning 1999 to 2018. In the National Health and Nutrition Examination Survey (NHANES) and the Behavioral Risk Factor Surveillance System (BRFSS) survey, participants were asked about prior stroke and active tobacco smoking. In NHANES, serum cotinine levels were available as a secondary measure of active smoking. We used multivariable logistic regression models for survey data to assess trends in active smoking among participants with and without prior stroke. Results- Among 49 375 participants in NHANES during 1999 to 2016 and 3 621 741 participants in BRFSS during 2011 to 2018, the prevalence of stroke was ≈3%. The overall prevalence of active smoking among stroke survivors was 24% in NHANES and 23% in BRFSS. Among individuals without prior stroke, the odds of smoking decreased over time in both NHANES (odds ratio, 0.95 per 2 years [95% CI, 0.93-0.96]) and BRFSS (odds ratio, 0.96 per year [95% CI, 0.96-0.96]). In contrast, there was no decrease in smoking among stroke survivors in NHANES (odds ratio, 1.00 [95% CI, 0.93-1.07]) or BRFSS (odds ratio, 0.99 [95% CI, 0.98-1.004]). Results were consistent in secondary analysis using biochemical ascertainment of active smoking in NHANES and in sensitivity analyses accounting for potential demographic changes in stroke epidemiology. Conclusions- In contrast to the general population, the prevalence of active smoking among stroke survivors has not decreased during the past 2 decades.
Electromagnetic field computation in planar integrated structures with a biaxial grounded slab (1993)

A method based on the full wall analysis (FWA) is presented to treat analytically and evaluate numerically the electromagnetic field sustained by an electric source inside an electrically biaxial grounded slab embedded in an isotropic homogeneous half-space in the presence of a perfectly conducting finite patch located at the interface. As a prerequisite, the electric spectral (Fourier) dyadic Green's function of the structure is developed and fully derived via the transmission-line analogy. Then the expression of the electric field in each region of the space in terms of the spectral electric Green's dyad is given. The use of the boundary conditions allows one to determine a spectral electric integral equation (SEIE) for the electric current density induced on the scatterer's surface. The solution of the SEIE is carried out in the general case of location of the electric sources with the patch parallel to the interface. Numerical computations are discussed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
A New Path: Scaling Vision-and-Language Navigation with Synthetic Instructions and Imitation Learning (2022)

Recent studies in Vision-and-Language Navigation (VLN) train RL agents to execute natural-language navigation instructions in photorealistic environments, as a step towards robots that can follow human instructions. However, given the scarcity of human instruction data and limited diversity in the training environments, these agents still struggle with complex language grounding and spatial language understanding. Pretraining on large text and image-text datasets from the web has been extensively explored but the improvements are limited. We investigate large-scale augmentation with synthetic instructions. We take 500+ indoor environments captured in densely-sampled 360 degree panoramas, construct navigation trajectories through these panoramas, and generate a visually-grounded instruction for each trajectory using Marky, a high-quality multilingual navigation instruction generator. We also synthesize image observations from novel viewpoints using an image-to-image GAN. The resulting dataset of 4.2M instruction-trajectory pairs is two orders of magnitude larger than existing human-annotated datasets, and contains a wider variety of environments and viewpoints. To efficiently leverage data at this scale, we train a simple transformer agent with imitation learning. On the challenging RxR dataset, our approach outperforms all existing RL agents, improving the state-of-the-art NDTW from 71.1 to 79.1 in seen environments, and from 64.6 to 66.8 in unseen test environments. Our work points to a new path to improving instruction-following agents, emphasizing large-scale imitation learning and the development of synthetic instruction generation capabilities.
Additive Manufacturing (AM) for Propulsion Component and System Applications (2021)

NASA MSFC is developing several areas of additive manufacturing (AM) for propulsion component and system applications. These developments include new AM process and material development, large-scale AM pathfinder component fabrication and hot-fire testing, AM-centric design methodology, modeling and simulation, post-processing advancements, and new design applications of AM in aerospace. Some specific areas NASA has focused is the development and commercial infusion of AM supply chain for materials such as copper-alloy GRCop (Cu-Cr-Nb), Hydrogen-resistant alloys (NASA HR-1, JBK-75), refractory-based alloys (C-103, W, Mo, new formulations), and bimetallic and multi-metallic AM processes. Additional efforts have been focused on large scale AM demonstrating complex thin-wall structures up to 60” diameter using directed energy deposition. Material characterization, properties and component hot-fire testing has been completed using many of these newly developed AM alloys/processes. NASA has also focused on improvements to post-processing including powder removal, surface enhancements, and support removal to further realize cost and schedule benefits to the overall supply chain. These developments are part of the NASA Rapid Analysis and Manufacturing Propulsion Technology (RAMPT), Low Cost Upper Stage Propulsion (LCUSP) and Long Life Additive Manufacturing (LLAMA) projects, in addition to internal IRAD, and external collaborations.
DOG1 Regulates Growth and IGFBP5 in Gastrointestinal Stromal Tumors (2013)

Gastrointestinal stromal tumors (GIST) are characterized by activating mutations of KIT or platelet-derived growth factor receptor α(PDGFRA), which can be therapeutically targeted by tyrosine kinase inhibitors (TKI) such as imatinib. Despite long-lasting responses, most patients eventually progress after TKI therapy. The calcium-dependent chloride channel DOG1 (ANO1/TMEM16A), which is strongly and specifically expressed in GIST, is used as a diagnostic marker to differentiate GIST from other sarcomas. Here, we report that loss of DOG1 expression occurs together with loss of KIT expression in a subset of GIST resistant to KIT inhibitors, and we illustrate the functional role of DOG1 in tumor growth, KIT expression, and imatinib response. Although DOG1 is a crucial regulator of chloride balance in GIST cells, we found that RNAi-mediated silencing or pharmacologic inhibition of DOG1 did not alter cell growth or KIT signaling in vitro. In contrast, DOG1 silencing delayed the growth of GIST xenografts in vivo. Expression profiling of explanted tumors after DOG1 blockade revealed a strong upregulation in the expression of insulin-like growth factor-binding protein 5 (IGFBP5), a potent antiangiogenic factor implicated in tumor suppression. Similar results were obtained after selection of imatinib-resistant DOG1- and KIT-negative cells derived from parental DOG1 and KIT-positive GIST cells, where a 5,000-fold increase in IGFBP5 mRNA transcripts were documented. In summary, our findings establish the oncogenic activity of DOG1 in GIST involving modulation of IGF/IGF receptor signaling in the tumor microenvironment through the antiangiogenic factor IGFBP5.
HVOF COATINGS OF DIAMALLOY 2002 AND DIAMALLOY 4010 ONTO STEEL: TENSILE AND BENDING RESPONSE OF COATINGS (2011)

HVOF coating of Diamalloy 2002 powders and Diamalloy 4010 powders as well as two‐layered coatings consisting of these powders is carried out. In the two‐layered structure, Diamalloy 4010 is sprayed at the substrate surface while Diamalloy 2002 is sprayed on the top of Diamalloy 4010 coating. The mechanical properties of the coatings are examined through tensile and three‐point bending tests. The coating microstructure and morphology are examined using the Scanning Electron Microscope (SEM), Energy Dispersive Spectroscopy (EDS) and X‐ray diffraction (XRD). It is found that the coating produced is free from defects including voids and cracks. The failure mechanism of coating during tensile and three‐point bending tests is mainly crack formation and propagation in the coating. The elastic modulus of coating produced from Diamalloy 2002 is higher than that of Diamalloy 4010 coating, which is due to the presence of 12% WC in the coating.
A CO&lt;SUB&gt;2&lt;/SUB&gt;- Laser- Pumped Para- H&lt;SUB&gt;2&lt;/SUB&gt; Raman Laser (1985)

Various aspects of stimulated rotational Raman scattering of a TEA-CO2 laser in para-H2 are studied with a liquid-N2 cooled multiple-pass cell. Line-tunable output from 13 to 30 μm is obtained by generating the 1st and 2nd Stokes waves. The 1st Stokes output energies for the 10 μm band pumping increase in proportion to the increase of the pump energies and the maximum conversion efficiency reaches 80%. On the other hand, the increase of the lst Stokes output for the 9 μm band pumping is limited because of generation of the 2nd Stokes wave. Beam crossing effect which is an important factor for achievement of high conversion efficiency in a multiple pass cell is analysed by numerical simulation. In addition, efficient conversion with the use of roomtemperature H2 is realised by improving optical condition.
Ionic Liquids as Electrolyte in Lithium Batteries: In Situ FTIRs Studies on the Use of Electrolyte Additives (2008)

The solid electrolyte interphase (SEI) formation on commercial graphite, KS6 TIMCAL, in two different ionic liquids, N-methyl-N-butyl pyrrolidinium bis(trifluoromethansulfonyl)imide (PYR14TFSI) and N-methyl-N-propylpyrrolidinium bis(fluorosulfonyl)imide (PYR13FSI) has been investigated. The role of the Vinylene Carbonate (VC) addition to the ionic liquid-based electrolyte has also been studied. To monitor the change at the electrode/electrolyte (IL) interface during the SEI formation, we took advantage of the in situ Fourier transform infrared spectroscopy (FTIRS). The results of the investigation performed at room temperature are presented in this manuscript.
Sélection de populations microbiennes dans les biofilms électroactifs (2014)

Les procédés bio-électrochimiques (BES pour « bioelectrochemical systems ») sont des procédés électrochimiques dans lesquels la réaction d’oxydation à l’anode et/ou de réduction à la cathode est catalysée par des microorganismes. L’exemple emblématique de ces nouveaux procédés est la pile à combustible microbienne (MFC pour « microbial fuel cell ») qui permet de coupler le traitement d’effluents organiques à la production directe d’électricité. Si ces procédés, dont le champ d’application ne cesse de s’élargir (production d’H2 ou de molécules plateformes), n’en sont encore qu’au stade de la recherche, ils connaissent un intérêt croissant depuis une quinzaine d’années et les premières applications industrielles pourraient voir le jour assez rapidement. Les microorganismes catalysant les réactions dans les BES sont capables d’échanger des électrons avec les électrodes qu’elles utilisent comme accepteur d’électrons dans le cas de réactions d’oxydation à l’anode, ou comme donneur d’électrons dans le cas de réactions de réduction à la cathode. Ils sont le plus souvent structurés en biofilms sur l’électrode et sont qualifiés d’électroactifs. Un des facteurs limitant de ces procédés est la difficulté à maitriser la formation d’un biofilm performant et stable dans le temps sur les électrodes. Nous nous sommes donc intéressés à la formation du biofilm sur des électrodes de graphite sous différents angles tels que le choix et la préparation de l’inoculum en fonction de la réaction à catalyser et des conditions opératoires, le développement de stratégies d’enrichissement ou encore la reconstruction de biofilms à partir de souches pures. Ces approches seront illustrées par différents exemples de travaux menés au LBE et mettant en évidence l’intérêt des BES comme modèles d’études pour le développement de stratégies de management des écosystèmes microbiens.
A novel human ex-vivo burn model and the local cooling effect of a bacterial nanocellulose-based wound dressing (2020)

Based on our results, BNC-based wound dressings cool a burn. Intradermal temperature as well as thermal damage of the tissue was reduced. The tested BNC-based wound dressing can be used without pre-cooling to cool a burn as well as to reduce the burn BNC-based wound progression through its evaporation cooling effect.
High-efficiency CdTe thin-film solar cells using metalorganic chemical vapor deposition techniques (1989)

Energy conversion efficiency of metalorganic chemical vapor deposited CdTe as an intrinsic active layer in n-i-p solar cell structures is reported. Small-area devices with efficiencies over 9% have been demonstrated. I–V characteristics, photospectral response, and the results of Auger profiling of structural composition for typical devices will be presented. Also presented are preliminary results on similar photovoltaic devices having Cd0.85Mn0.15Te in place of CdTe as an i layer.