gebhart/patrepeval huggingface dataset:
Incorporation of strontium-containing bioactive particles into PEOT/PBT electrospun scaffolds for bone tissue regeneration (2023)

The combination of biomaterials and bioactive particles has shown to be a successful strategy to fabricate electrospun scaffolds for bone tissue engineering. Among the range of bioactive particles, hydroxyapatite and mesoporous bioactive glasses (MBGs) have been widely used for their osteoconductive and osteoinductive properties. Yet, the comparison between the chemical and mechanical characteristics as well as the biological performances of these particle-containing scaffolds have been characterized to a limited extent. In this work, we fabricated PEOT/PBT-based composite scaffolds incorporating either nanohydroxyapatite (nHA), strontium-containing nanohydroxyapatite (nHA_Sr) or MBGs doped with strontium ions up to 15 wt./vol% and 12,5 wt./vol% for nHA and MBG, respectively. The composite scaffolds presented a homogeneous particle distribution. Morphological, chemical and mechanical analysis revealed that the introduction of particles into the electrospun meshes caused a decrease in the fiber diameter and mechanical properties, yet maintaining the hydrophilic nature of the scaffolds. The Sr<sup>2+</sup> release profile differed according to the considered system, observing a 35-day slowly decreasing release from strontium-containing nHA scaffolds, whereas MBG-based scaffolds showed a strong burst release in the first week. In vitro, culture of human bone marrow-derived mesenchymal stromal cells (hMSCs) on composite scaffolds demonstrated excellent cell adhesion and proliferation. In maintenance and osteogenic media, all composite scaffolds showed high mineralization as well as expression of Col I and OCN compared to PEOT/PBT scaffolds, suggesting their ability to boost bone formation even without osteogenic factors. The presence of strontium led to an increase in collagen secretion and matrix mineralization in osteogenic medium, while gene expression analysis showed that hMSCs cultured on nHA-based scaffolds had a higher expression of OCN, ALP and RUNX2 compared to cells cultured on nHA_Sr scaffolds in osteogenic medium. Yet, cells cultured on MBGs-based scaffolds showed a higher gene expression of COL1, ALP, RUNX2 and BMP2 in osteogenic medium compared to nHA-based scaffolds, which is hypothesized to lead to high osteoinductivity in long term cultures.
A New Quantitative Method for the Non-Invasive Documentation of Morphological Damage in Paintings Using RTI Surface Normals (2014)

In this paper we propose a reliable surface imaging method for the non-invasive detection of morphological changes in paintings. Usually, the evaluation and quantification of changes and defects results mostly from an optical and subjective assessment, through the comparison of the previous and subsequent state of conservation and by means of condition reports. Using quantitative Reflectance Transformation Imaging (RTI) we obtain detailed information on the geometry and morphology of the painting surface with a fast, precise and non-invasive method. Accurate and quantitative measurements of deterioration were acquired after the painting experienced artificial damage. Morphological changes were documented using normal vector images while the intensity map succeeded in highlighting, quantifying and describing the physical changes. We estimate that the technique can detect a morphological damage slightly smaller than 0.3 mm, which would be difficult to detect with the eye, considering the painting size. This non-invasive tool could be very useful, for example, to examine paintings and artwork before they travel on loan or during a restoration. The method lends itself to automated analysis of large images and datasets. Quantitative RTI thus eases the transition of extending human vision into the realm of measuring change over time.
Measurement and Modeling of the Origins of Starvation of Congestion-Controlled Flows in Wireless Mesh Networks (2009)

Significant progress has been made in understanding the behavior of TCP and congestion-controlled traffic over CSMA-based multihop wireless networks. Despite these advances, however, no prior work identified severe throughput imbalances in the basic scenario of mesh networks, in which a one-hop flow contends with a two-hop flow for gateway access. In this paper, we demonstrate via real network measurements, testbed experiments, and an analytical model that starvation exists in such a scenario; i.e., the one-hop flow receives most of the bandwidth, while the two-hop flow starves. Our analytical model yields a solution consisting of a simple contention window policy that can be implemented via standard mechanisms defined in IEEE 802.11e. Despite its simplicity, we demonstrate through analysis, experiments, and simulations that the policy has a powerful effect on network-wide behavior, shifting the network's queuing points, mitigating problematic MAC and transport behavior, and ensuring that TCP flows obtain a fair share of the gateway bandwidth, irrespective of their spatial location.
Tau Differentially Regulates the Transport of Early Endosomes and Lysosomes (2021)

Abstract Microtubule-associated proteins (MAPs) modulate the motility of kinesin and dynein along microtubules to control the transport of vesicles and organelles. The neuronal MAP tau inhibits kinesin-dependent transport. Phosphorylation of tau at tyrosine 18 by fyn kinase results in weakened inhibition of kinesin-1. We examined the motility of early endosomes and lysosomes in cells expressing wild-type (WT) tau and phosphomimetic Y18E tau. Lysosome motility is strongly inhibited by tau. Y18E tau preferentially inhibits lysosomes in the cell periphery, while centrally located lysosomes are less affected. Early endosomes are more sensitive to tau than lysosomes, and are inhibited by both WT and Y18E tau. Our results show that different cargoes have disparate responses to tau, likely governed by the types of kinesin motors driving their transport. In support of this model, kinesin-1 and -3 are strongly inhibited by tau while kinesin-2 and dynein are less affected. In contrast to kinesin-1, we find that kinesin-3 is strongly inhibited by phosphorylated tau.
Experimental Study of Water Liquid-Vapor Two-Phase Pressure Drop Across an Array of Staggered Micropin-Fins (2009)

This study concerns pressure drop of adiabatic water liquid-vapor two-phase flow across an array of 1950 staggered square micropin-fins having a 200×200 μm cross section by 670 μm height. The ratios of longitudinal pitch and transverse pitch to pin-fin equivalent diameter are equal to 2. An inline immersion heater upstream of the micropin-fin test module was employed to produce liquid-vapor two-phase mixture, which flowed across the micropin-fin array. The test module was well insulated to maintain adiabatic condition. Four maximum mass velocities of 184 kg/m2 s, 235 kg/m2 s, 337 kg/m2 s, and 391 kg/m2 s, and a range of vapor qualities for each maximum mass velocity were tested. Measured pressure drop increases drastically with increasing vapor quality. Nine existing two-phase pressure drop models and correlations were assessed. The Lockhart–Martinelli correlation for laminar liquid-laminar vapor combination in conjunction with a single-phase friction factor correlation proposed for the present micropin-fin array provided the best agreement with the data.
Biocomputational Approaches in Protein-Based Drug Design (2020)

Biocomputational approaches in the rational design of bioactive proteins and peptides are driven by a general understanding of tertiary structure, electrostatic interactions, hydrogen bonding, hydrophobic interactions, desolvation effects, and cooperative motions of interacting ligands and receptors, backed up by data base searches and simulation techniques. This chapter presents a brief summary of various protein prediction strategies commonly used in computational biology, including computational approaches for predicting and energetically analyzing protein structures and for analyzing nuclear magnetic resonance (NMR) data. There is a wealth of software and methods available for computer-assisted drug design which was recently reviewed that is either the same or complementary to protein-based drug design. The design strategy incorporated NMR data, energy minimization, template forcing, and molecular dynamics. Current biocomputational approaches to peptide-based drug design combine knowledge-based systems involving crystallographic, NMR, and sequence data bases with experimentally determined constraints, conformational energy analysis, and molecular modeling.
Adrenergic Receptor Function is Different in Male and Female Patients with Essential Hypertension (1979)

As plasma norepinephrine (NE) levels may be similar in hypertensive and normotensive subjects, the sensitivity of adrenergic receptors was investigated in patients with essential hypertension and normotensive subjects of similar age and sex. Alpha-adrenergic receptor sensitivity was measured in platelets by the specific binding of [3H]dihydroergocryptine and the NE inhibition of prostaglandin E1 (PGE1)-stimulated cyclic AMP (cAMP) production. The number of alpha-adrenergic receptors in platelets from hypertensive women was 1.5 times that in the platelets from normotensive ones, with no differences between hypertensive and normotensive women or between men and women in the affinity of the alpha-adrenergic receptor for [3H]dihydroergocryptine. PGE1-stimulated cAMP production was half as great in hypertensive as in normotensive men, while NE inhibition of PGE1-stimulated cAMP production was similar in hypertensive and normotensive men and women. [3H]Dihydroergoeryptine binding in female hypertensives, and PGE1-stimulated cAMP in male hypertensives did not differ from that in sex-matched controls. The sensitivity of the beta-adrenergic receptor, measured by [3H]dihydroalprenolol binding and cAMP production was similar in hypertensive and normotensive subjects.
Molecular Studies of Human Renin Structure and Synthesis using Monoclonal Antibodies (1987)

Monoclonal antibodies are employed as molecular probes to study human renin structure and synthesis. In this study, we observed that the active site peptide directed antibody did not bind native renin consistent with the hypothesis that the active site is at the bottom of a cleft. On the other hand, sequences that determine substrate or species specificity are on the surface of the molecule. Based on studies using antibodies directed to N-or C-teminus of prosegment of prorenin we propose that the N-terminus is hidden within the conformation of the molecule, whereas the C-terminus (activation site) is on the surface. Additional studies using monoclonal antibodies demonstrate that the human kidney contains a 50 kd prorenin, a 42 kd N-terminal truncated prorenin and a 38 kd mature renin. Our data suggest that prorenin is processed to renin by two or more sequential N-terminal cleavages.
Reducible oxide based catalysts (2023)

A catalyst is disclosed herein. The catalyst includes a reducible oxide support and at least one noble metal fixed on the reducible oxide support. The noble metal(s) is loaded on the support at a substantially constant temperature and pH.
Suppressor activity among CD4+,CD25++ T cells is discriminated by membrane‐bound tumor necrosis factor α (2008)

Our data indicate that the absence of membrane-bound TNFalpha on CD4+,CD25++ T cells can be used to characterize and enrich for Treg cells with maximal suppressor potency. Enrichment of membrane-bound TNFalpha-negative CD4+,CD25+ cells in the CD4+,CD25++ T cell compartment may contribute to restoring the compromised suppressive ability of CD4+,CD25++ T cell populations in RA patients after anti-TNFalpha treatment.
Data from Paradoxical Activation of T Cells via Augmented ERK Signaling Mediated by a RAF Inhibitor (2023)

&lt;div&gt;Abstract&lt;p&gt;RAF inhibitors selectively block extracellular signal–regulated kinase (ERK) signaling in BRAF-mutant melanomas and have defined a genotype-guided approach to care for this disease. RAF inhibitors have the opposite effect in BRAF wild-type tumor cells, where they cause hyperactivation of ERK signaling. Here, we predict that RAF inhibitors can enhance T-cell activation, based on the observation that these agents paradoxically activate ERK signaling in BRAF wild-type cells. To test this hypothesis, we have evaluated the effects of the RAF inhibitor BMS908662 on T-cell activation and signaling &lt;i&gt;in vitro&lt;/i&gt; and &lt;i&gt;in vivo&lt;/i&gt;. We observe that T-cell activation is enhanced in a concentration-dependent manner and that this effect corresponds with increased ERK signaling, consistent with paradoxical activation of the pathway. Furthermore, we find that the combination of BMS908662 with cytotoxic T-lymphocyte antigen 4 (CTLA-4) blockade &lt;i&gt;in vivo&lt;/i&gt; potentiates T-cell expansion, corresponding with hyperactivation of ERK signaling in T cells detectable &lt;i&gt;ex vivo&lt;/i&gt;. Finally, this combination demonstrates superior antitumor activity, compared with either agent alone, in two transplantable tumor models. This study provides clear evidence that RAF inhibitors can modulate T-cell function by potentiating T-cell activation &lt;i&gt;in vitro&lt;/i&gt; and &lt;i&gt;in vivo&lt;/i&gt;. Paradoxical activation of ERK signaling in T cells offers one mechanism to explain the enhanced antitumor activity seen when RAF inhibitors are combined with CTLA-4 blockade in preclinical models. &lt;i&gt;Cancer Immunol Res; 2(1); 70–79. ©2013 AACR&lt;/i&gt;.&lt;/p&gt;&lt;/div&gt;
Droplet motions fill a periodic table (2019)

Drawing parallels to the symmetry breaking of atomic orbitals used to explain the periodic table of chemical elements; here we introduce a periodic table of droplet motions, also based on symmetry breaking but guided by a recent droplet spectral theory. By this theory, higher droplet mode shapes are discovered and a wettability spectrometer is invented. Motions of a partially wetting liquid on a support have natural mode shapes, motions ordered by kinetic energy into the periodic table, each table characteristic of the spherical-cap drop volume and material parameters. For water on a support having a contact angle of about 60°, the first 35 predicted elements of the periodic table are discovered. Periodic tables are related one to another through symmetry breaking into a two-parameter family tree.
A comparative study of dc biased RF impedance probes and Langmuir probes for the measurement of processing plasma parameters (2012)

In this work, we compare measurements of electron density (ne), plasma potential (p), electron temperature (Te), and electron energy distribution function (EEDF or f(E)) made with a dc biased RF probe and a Langmuir probe. The measurements show good agreement between the two probes across an order of magnitude in plasma density.
Knock down of p53 or its ubiquitin ligase E6AP does not affect the sensitivity of human papillomavirus-positive cervical cancer cells to cisplatin. (2012)

The persistent infection with high risk human papillomaviruses (hrHPV) is a necessary risk factor for the development of cervical cancer, which is the second most frequent cancer in women worldwide. Cisplatin-based radiotherapy represents the current treatment regimen. However, the results for advanced and recurrent disease are far from optimal. Since almost all cervical cancers contain wild type (wt) p53, which is degraded by the complex of hrHPV E6 and the ubiquitin ligase E6AP, we addressed if the reconstitution of p53 via silencing of E6AP sensitizes cervical cancer cells towards cisplatin treatment. For this we established and characterized two novel cervical cancer cell lines that contain integrated HPV16 genomes. Long-term established HeLa and SiHa cells and the novel cervical cancer cell lines at low passage numbers were treated with different concentrations of cisplatin. Cell viability was measured by the WST-1 assay. In addition, single cisplatin treatment was combined with the silencing of E6AP or p53. The comparison to HeLa and SiHa cells revealed a higher sensitivity of the novel cell lines to cisplatin treatment, which caused p53 accumulation and transcriptional induction of p21. Silencing of E6AP further increased p53 protein levels, but had no effect on cell viability when combined with cisplatin treatment. Interestingly, silencing of p53 had also no effect. We therefore conclude that reactivation of p53 via silencing of E6AP does not increase the sensitivity of cervical cancer cells towards cisplatin treatment.
Table S2 from The Hsp27-Mediated IkBα-NFκB Signaling Axis Promotes Radiation-Induced Lung Fibrosis (2023)

&lt;p&gt;The parameter description using the flexiVentTM system measurements&lt;/p&gt;
Supplementary Table 4 from Reactive Oxygen Species, DNA Damage, and Error-Prone Repair: A Model for Genomic Instability with Progression in Myeloid Leukemia? (2023)

Supplementary Table 4 from Reactive Oxygen Species, DNA Damage, and Error-Prone Repair: A Model for Genomic Instability with Progression in Myeloid Leukemia?
Design and VLSI implementation of CSD filter for pre-processing of image signals (2002)

In this paper, we investigate the design and VLSI implementation of CSD (canonic signed digit) coefficient filters for high speed digital filtering. First, CSD coefficient filters are designed, which can be used as format conversion filters in place of the ones employed for the MPEG2 TM5 (test model 5). It is shown that the proposed CSD filters perform better than the conventional one, while having lower hardware complexity. Second, the pipelined bit-serial and bit-parallel architectures for the CSD filter are proposed and verified through the VHDL simulation.
Development and validation of high‐performance liquid chromatography–tandem mass spectrometry assay for 6‐(3‐benzoyl‐ureido)‐hexanoic acid hydroxyamide, a novel HDAC inhibitor, in mouse plasma for pharmacokinetic studies (2007)

A liquid chromatography/tandem mass spectrometric method for the quantification of 6-(3-benzoyl-ureido)-hexanoic acid hydroxyamide (EX-2), a novel histone deacetylase (HDAC) inhibitor, in mouse plasma was developed to support in-house pharmacokinetic (PK) studies in the lead optimization stage. In order to determine the PK parameters for EX-2 in comparison to other HDAC inhibitors such as suberoylanilide hydroxamic acid (SAHA), PXD-101 and LBH-589, which are currently in different stages of clinical trials, research-grade bio-analytical method validations were carried out for EX-2 and these reference HDAC inhibitors, which were synthesized by in-house medicinal chemists. The components of validation consisted of specificity, extraction efficiency, signal-response of calibration standards, lower limit of quantification, autosampler stability and accuracy and precision of quality control samples. The validated LC/MS/MS methods were accurate and precise. The calibration curve ranged from 1 to 1600 ng/mL for all the analytes. The methods developed were used to quantify EX-2 and other HDAC inhibitors in mouse plasma obtained from pharmacokinetic studies. The results suggest that EX-2 has better PK parameters compared with the reference drugs and is a promising drug development candidate.
Interactive Classification of Sound Objects for Polyphonic Electro-Acoustic Music Annotation (2011)

International audience
[Solution] Mir-BFT: Scalable and Robust BFT for Decentralized Networks (2022)

This paper presents Mir-BFT, a robust Byzantine fault-tolerant (BFT) total order broadcast protocol aimed at maxi-mizing throughput on wide-area networks (WANs), targetingdeployments in decentralized networks, such as permissionedand Proof-of-Stake permissionless blockchain systems.Mir-BFT is the first BFT protocol that allows multiple lead-ers to propose request batches independently (i.e., parallelleaders), while effectively precluding performance degrada-tion due to request duplication by rotating the assignmentof a partitioned request hash space to leaders. As this mech-anism removes the single-leader bandwidth bottleneck andexposes a computation bottleneck related to authenticatingclients even on a WAN, our protocol further boosts through-put using a client signature verification sharding optimization.Our evaluation shows that Mir-BFT outperforms state-of-the-art single-leader protocols and orders more than 60000 signedBitcoin-sized (500-byte) transactions per second on a widelydistributed setup (100 nodes, 1 Gbps WAN) with typical la-tencies of few seconds. Moreover, our evaluation exposesthe impact of duplicate requests on parallel leader protocolswhich Mir-BFT eliminates. We also evaluate Mir-BFT un-der different crash and Byzantine faults, demonstrating itsperformance robustness.Mir-BFT relies on classical BFT protocol constructs, whichsimplifies reasoning about its correctness. Specifically, Mir-BFT is a generalization of the celebrated and scrutinizedPBFT protocol. In a nutshell, Mir-BFT follows PBFT “safety-wise”, with changes needed to accommodate novel featuresrestricted to PBFT liveness.
Evaluation of Inactivation Efficacy of Four Disinfectants against Mouse Hepatitis Virus (2007)

Objective To evaluate the efficacy for 4 disinfectants in inactivation of mouse hepatitis virus(MHV).Methods According to the 'Disinfectants Technical Guidelines',2002 ed,neutralization and MHV inactivation tests were used to evaluate their antiviral activity.Results After treatment with MHV-A59 for 5 min and 10 min,the inactivation logarithmic value of peracetic acid disinfectant,iodophor,sodium dichloroisocyanurate were higher than 4.The inactivation logarithmic value of benzalkonium bromide was lower than 4,indicating a low inactivation efficacy,which could not satisfy the requirements of guidelines.Conclusion Peracetic acid disinfectant,iodophor and sodium dichloroisocyanurate have good efficacy in inactivation of MHV-A59.
"Reduced" analogs of puromycin. Synthesis of 3'-O-(L-2-amino-3-phenylpropyl)-N6,N6-dimethyladenosine and the corresponding 2' isomer (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT"Reduced" analogs of puromycin. Synthesis of 3'-O-(L-2-amino-3-phenylpropyl)-N6,N6-dimethyladenosine and the corresponding 2' isomerTetsuo Kato and Jiri ZemlickaCite this: J. Org. Chem. 1980, 45, 20, 4006–4010Publication Date (Print):September 1, 1980Publication History Published online1 May 2002Published inissue 1 September 1980https://doi.org/10.1021/jo01308a012RIGHTS & PERMISSIONSArticle Views100Altmetric-Citations12LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (769 KB) Get e-Alerts Get e-Alerts