gebhart/patrepeval huggingface dataset:
Inhibition of cis-diammine-1,1-cyclobutane dicarboxylatoplatinum(II)-induced DNA interstand cross-link removal and potentiation of cis-diammine-1,1-cyclobutane dicarboxylatoplatinum(II) cytotoxicity by hydroxyurea and 1-beta-D-arabinofuranosylcytosine. (1991)

1-beta-D-Arabinofuranosylcytosine (ara-C) and hydroxyurea (HU) were investigated as potential DNA repair inhibitors with cis-diammine-1,1-cyclobutane dicarboxylatoplatinum(II) (CBDCA). HU plus ara-C, known inhibitors of DNA excision-repair, had previously been found to produce cytotoxic synergy and delayed removal of DNA interstrand cross-links with cis-diamminedichloroplatinum(II) (DDP). Since CBDCA and DDP share a common active intermediate, it should be possible to reproduce this interaction with CBDCA. However, the stable dicarboxylate chelate ring structure of CBDCA results in kinetics that differ significantly from those of DDP, due to slower hydrolysis to the active species. DNA adducts form more slowly, with interstand cross-links peaking approximately 12-h later and disappearing more gradually than in the case of DDP. It was therefore expected that a longer antimetabolite exposure might be required for repair inhibition with CBDCA. The 12-h exposure to HU plus ara-C previously found effective with DDP produced no cytotoxic synergy with a 2-h CBDCA exposure. Lengthening the antimetabolite treatment to 24 h resulted in approximately 1 log of synergistic toxicity, while a 24-h simultaneous exposure to HU, ara-C, and CBDCA resulted in 2 logs. Cells exposed to all three drugs showed a 2- to 3-fold greater level of interstrand cross-links after 36- to 48-h of incubation following drug removal, compared to CBDCA alone. Taken together, these findings suggest that HU plus ara-C modulates the repair of platinum-DNA adducts and establishes an effective in vitro schedule at clinically achievable concentrations for the use of those antimetabolites with CBDCA.
Risk Factors for <i>Toxoplasma gondii</i> Exposure in Semiaquatic Mammals in a Freshwater Ecosystem (2015)

We assessed risk factors for Toxoplasma gondii exposure in semiaquatic mammals in east-central Illinois, US. This agricultural region has extensive drainage systems that could potentially transport T. gondii oocysts into the watershed. We used muskrats (Ondatra zibethicus) and American mink (Neovison vison) as sentinels of watershed contamination. We predicted individuals from larger subwatersheds would more likely be antibody-positive for T. gondii, as they were exposed to drainage from larger areas. We also evaluated amount of urban land cover within the subwatershed, proximity to farmsteads, and age of individuals in competing models of T. gondii infection. Antibodies to T. gondii were assayed in animal sera by modified agglutination tests (titer 25 or higher) and detected in 18 (60%) of 30 muskrats and 20 (77%) of 26 mink. Infection rates were ≥1.7 times higher than those typical for mammals in upland habitats in this region. Subwatershed size and age class were important predictors of T. gondii infection in muskrats (R(2) = 0.35). Models incorporating urban land cover and proximity to farmsteads had little support. None of our models of antibody prevalence in mink were well supported, possibly because mink are less strictly associated with riparian habitats. Because ~91% of our study area is devoted to agricultural production and urbanization, transport of T. gondii into freshwater ecosystems is likely facilitated by modified drainage practices common in these areas.
Development and Atmospheric Testing of a High Hydrogen FlameSheet™ Combustor for the OP16 Gas Turbine (2021)

Abstract Gas turbines with a combustion system for hydrogen operation offer a low carbon solution to support the stability of the energy grid. This provides a solution capturing the needs for energy storage, in the form of hydrogen, and flexible power generation. Fuel flexibility is a key requirement to reduce the operational risks in case hydrogen is not available, whereby hydrogen can be combined with other conventional or alternative fuels. A key issue to achieve 100% hydrogen combustion with low emissions is to prevent flashback. To address the challenges, a project consortium was set-up consisting of gas turbine equipment manufacturers, academia and end-users. The major objective is to develop a cost-effective, ultra-low emissions (sub 9 ppm NOx and CO) combustion system for gas turbines in the 1–300 MW output range, including the 1.85 MWe OPRA OP16 gas turbine. At the center of this innovative high-technology project is the patented and novel aerodynamic trapped vortex FlameSheet™ combustion technology platform. Burner concepts based on an aerodynamically trapped vortex flame stabilization have a higher resistance towards flame blowout than conventional swirl stabilized burners. This paper will present the results of the first phase of the project, whereby atmospheric testing of the upgraded FlameSheet™ combustor has been performed operating on natural gas, hydrogen and mixtures thereof. The optimized combustor configurations demonstrated a wide load range on 100% hydrogen, and these results will be presented.
Kommunikation und Bildverarbeitung in der Automation (2020)

Dieser Open-Access-Tagungsband stellt die neuesten Forschungsergebnisse auf den Gebieten der industriellen Kommunikationstechnik und Bildverarbeitung vor. Er enthält die besten Beiträge des 9. Jahreskolloquiums KommA 2018 und des 6. Jahreskolloquiums BVAu 2018.
Propagation of spatially partially coherent emission from a vertical-cavity surface-emitting laser (2006)

Recently we observed a strong reduction of spatial coherence of the emission of large-aperture vertical-cavity surface-emitting lasers when they are driven by microsecond electrical pulses [Opt. Express 13, 9337 (2005)]. We study the influence of this partial spatial coherence on the propagation characteristics. The spatial decoherence manifests itself in the formation of a Gaussian far-field intensity distribution. We measure the transverse pulse profile from near to far field and see that the shape-invariant far-field regime starts after 140 microm in the pulsed regime as opposed to several centimeters in continuous wave operation. This value matches quantitatively calculations made with a novel partially coherent propagation model without any free fitting parameters.
Dendritic cells with TGF-β1 differentiate naïve CD4<sup>+</sup>CD25<sup>−</sup>T cells into islet-protective Foxp3<sup>+</sup>regulatory T cells (2007)

CD4(+)CD25(+)Foxp3(+) regulatory T cells (T regs) are important for preventing autoimmune diabetes and are either thymic-derived (natural) or differentiated in the periphery outside the thymus (induced). Here we show that beta-cell peptide-pulsed dendritic cells (DCs) from nonobese diabetic (NOD) mice can effectively induce CD4(+)CD25(+)Foxp3(+) T cells from naïve islet-specific CD4(+)CD25(-) T cells in the presence of TGF-beta1. These induced, antigen-specific T regs maintain high levels of clonotype-specific T cell receptor expression and exert islet-specific suppression in vitro. When cotransferred with diabetogenic cells into NOD scid recipients, T regs induced with DCs and TGF-beta1 prevent the development of diabetes. Furthermore, in overtly NOD mice, these cells are able to significantly protect syngeneic islet grafts from established destructive autoimmunity. These results indicate a role for DCs in the induction of antigen-specific CD4(+)CD25(+)Foxp3(+) T cells that can inhibit fully developed autoimmunity in a nonlymphopoenic host, providing an important potential strategy for immunotherapy in patients with autoimmune diabetes.
Lithography line productivity impact using Cymer GLX technology (2009)

Leading-edge scanners in fabs worldwide have particularly high system utilization and require peak levels of system throughput and availability. Laser gas exchanges typically occur daily on these systems (or every 100M pulses or less), with each exchange lasting up to 20 minutes. This downtime has a direct negative effect on availability, and if it is reduced, the productivity of the litho cell increases. This paper will outline the immediate success fabs have experienced after equipping scanners with Cymer's Gas Lifetime eXtension (GLX<sup>TM</sup>) technology, which increases scanner availability by extending the time between excimer laser gas exchanges by a factor of more than 10. To date, more than 100 leading-edge scanners feature Cymer's GLX technology, which has improved light source availability by more than 1.5 percent. Moreover, multiple chipmakers report more than 2 percent improvement in litho cell productivity due to GLX, corresponding to 2000 wafers/month increase for a 100,000 wafers/month fab. The increase in measured productivity is the leveraged benefit of reducing process interruptions around the refill cycle GLX technology extends the shot-based interval between gas refills to 1 billion pulses for Cymer's XLA light sources, and provides excellent stability in key optical performance parameters, such as bandwidth and dose stability over the entire gas life. This paper will provide extensive performance data during extended light source operation on litho cells equipped with GLX technology, and multiple use scenarios will be examined, including usage at memory and logic fabs. The paper will also discuss the performance of GLX2<sup>TM</sup> technology which further extends the maximum time between light source gas exchanges from 1B pulses to 2B pulses, and reduces downtime associated with gas refills by a factor of 20. The stability and productivity benefits of this new technology can be realized under all light source utilization scenarios. With GLX2, the refill interval at high utilization chipmakers is 3 weeks, and 4-8 weeks at lower utilization customers. Metrics illustrating the success of each of these capabilities will be presented. The second-generation of GLX technology was launched in July 2008 after chipmakers responded favorably to GLX performance metrics.
An overview of the Meteracom Project (2019)

We overview the 3-year Meteracom project which<br/>will provide traceability to the SI for THz communication<br/>measurement parameters. The key objectives are to develop new<br/>metrological methods to characterize the measurement systems,<br/>system components and propagation channels. The final<br/>objective is to develop metrology for functionality and signal<br/>integrity of THz communication systems; particularly device<br/>discovery and beam tracking, determination of physical layer<br/>parameters for digital transmission and real-time performance<br/>evaluation.
Design of a fluorescent ligand targeting the S-adenosylmethionine binding site of the histone methyltransferase MLL1 (2015)

The histone methyltransferase MLL1 has been linked to translocation-associated gene fusion in childhood leukemias and is an attractive drug target. High-throughput biochemical analysis of MLL1 methyltransferase activity requires the production of at least a trimeric complex of MLL1, RbBP5 and WDR5 to elicit robust activity. Production of trimeric and higher order MLL1 complexes in the quantities and reproducibility required for high-throughput screening presents a significant impediment to MLL1 drug discovery efforts. We present here a small molecule fluorescent ligand (FL-NAH, 6) that is able to bind to the S-adenosylmethionine (SAM) binding site of MLL1 in a manner independent of the associated complex members. We have used FL-NAH to develop a fluorescence polarization-based SAM displacement assay in a 384-well format targeting the MLL1 SET domain in the absence of associated complex members. FL-NAH competes with SAM and is displaced from the MLL1 SET domain by other SAM-binding site ligands with Kdisp values similar to the higher-order complexes, but is unaffected by the H3 peptide substrate. This assay enables screening for SAM-competitive MLL1 inhibitors without requiring the use of trimeric or higher order MLL1 complexes, significantly reducing screening time and cost.
An Instrumented Apartment to Monitor Human Behavior: A Pilot Case Study in the NeuroTec Loft (2022)

For patients suffering from neurodegenerative disorders, the behavior and activities of daily living are an indicator of a change in health status, and home-monitoring over a prolonged period of time by unobtrusive sensors is a promising technology to foster independent living and maintain quality of life. The aim of this pilot case study was the development of a multi-sensor system in an apartment to unobtrusively monitor patients at home during the day and night. The developed system is based on unobtrusive sensors using basic technologies and gold-standard medical devices measuring physiological (e.g., mobile electrocardiogram), movement (e.g., motion tracking system), and environmental parameters (e.g., temperature). The system was evaluated during one session by a healthy 32-year-old male, and results showed that the sensor system measured accurately during the participant's stay. Furthermore, the participant did not report any negative experiences. Overall, the multi-sensor system has great potential to bridge the gap between laboratories and older adults' homes and thus for a deep and novel understanding of human behavioral and neurological disorders. Finally, this new understanding could be utilized to develop new algorithms and sensor systems to address problems and increase the quality of life of our aging society and patients with neurological disorders.
Optimizing Remote Accesses for Offloaded Kernels: Application to High-Level Synthesis for FPGA (2013)

Some data- and compute-intensive applications can be accelerated by offloading portions of codes to platforms such as GPGPUs or FPGAs. However, to get high performance for these kernels, it is mandatory to restructure the application, to generate adequate communication mechanisms for the transfer of remote data, and to make good usage of the memory bandwidth. In the context of the high-level synthesis (HLS), from a C program, of hardware accelerators on FPGA, we show how to automatically generate optimized remote accesses for an accelerator communicating to an external DDR memory. Loop tiling is used to enable block communications, suitable for DDR memories. Pipelined communication processes are generated to overlap communications and computations, thereby hiding some latencies, in a way similar to double buffering. Finally, not only intra-tile but also inter-tile data reuse is exploited to avoid remote accesses when data are already available in the local memory. Our first contribution is to show how to generate the sets of data to be read from (resp. written to) the external memory just before (resp. after) each tile so as to reduce communications and reuse data as much as possible in the accelerator. The main difficulty arises when some data may be (re)defined in the accelerator and should be kept locally. Our second contribution is an optimized code generation scheme, entirely at source-level, i.e., in C, that allows us to compile all the necessary glue (the communication processes) with the same HLS tool as for the computation kernel. Both contributions use advanced polyhedral techniques for program analysis and transformation. Experiments with Altera HLS tools demonstrate how to use our techniques to efficiently map C kernels to FPGA.
Enhancement Of Dielectric Permittivity And Electromechanical Response In Silicone Elastomers: Molecular Grafting Of Organic Dipoles To The Macromolecular Network (2011)

Abstract A novel method is established for permittivity enhancement of a silicone matrix for dielectric elastomer actuators (DEAs) by molecular level modifications of the elastomer matrix. A push‐pull dipole is synthesized to be compatible with the silicone crosslinking chemistry, allowing for direct grafting to the crosslinker molecules in a one‐step film formation process. This method prevents agglomeration and yields elastomer films that are homogeneous down to the molecular level. The dipole‐to‐silicone network grafting reaction is studied by FTIR. The chemical, thermal, mechanical and electrical properties of films with dipole contents ranging from 0 wt% to 13.4 wt% were thoroughly characterized. The grafting of dipoles modifies the relative permittivity and the stiffness, resulting in the actuation strain at a given electrical field being improved by a factor of six.
Identification of a uveitogenic cyanogen bromide peptide of bovine retinal S‐antigen and preparation of a uveitogenic, peptide‐specific T cell line (1987)

Peptide fragments of bovine retinal S-antigen produced by cyanogen bromide (CB) digestion have been purified and tested for their ability to induce experimental autoimmune uveoretinitis (EAU) and pinealitis (EAP) in Lewis rats. Following immunization with the various peptides in complete Freund's adjuvant, one of the peptides, CB123, was found to be potently uveitogenic. A CB123-specific, class II restricted T helper lymphocyte line (R208) prepared from one of the CB123 peptide-immunized animals by repeated in vitro selection with purified CB123 was able to transfer severe EAU and EAP to naive rats. Peptides CB36, CB46, CB51 and CB66 were immunogenic as assessed by the presence of antibodies detected in the enzyme-linked immunosorbent assay and sensitized lymphocytes found in [3H]thymidine incorporation assays using lymphocytes from the peptide-immunized rats, but they did not induce significant EAU or EAP. Antibodies induced by immunization with the peptides also bound intact S-antigen, unlike the lymphocytes which were only weakly responsive to S-antigen. Cross-reactivity of antibodies and lymphocytes from CB51 and CB66 immune animals suggested that these peptides overlap and contain a common epitope. No measurable immunity to any CB peptide or intact S-antigen was found in the animals immunized with peptide CB26. Although a T cell line (R17) raised to human S-antigen was also able to transfer EAU, it was weakly responsive to the CB123 peptide; also, the R208 line did not respond well to human S-antigen. Since both human and bovine S-antigen are uveitogenic, these results suggest that the uveitogenic epitope in CB123, which is of bovine origin, is not the same as the uveitogenic epitope of the human antigen.
Personalized crime location prediction (2016)

Crime reduction and prevention strategies are vital for policymakers and law enforcement to face inevitable increases in urban crime rates as a side effect of the projected growth of urban population by the year 2030. Studies conclude that crime does not occur uniformly across urban landscapes but concentrates in certain areas. This phenomenon has drawn attention to spatial crime analysis, primarily focusing on crime hotspots , areas with disproportionally higher crime density. In this paper, we present CrimeTracer 1 , a personalized random walk-based approach to spatial crime analysis and crime location prediction outside of hotspots. We propose a probabilistic model of spatial behaviour of known offenders within their activity spaces . Crime Pattern Theory concludes that offenders, rather than venture into unknown territory, frequently select targets in or near places they are most familiar with as part of their activity space. Our experiments on a large real-world crime dataset show that CrimeTracer outperforms all other methods used for location recommendation we evaluate here.
Precore wild-type DNA and immune complexes persist in chronic hepatitis B after seroconversion: No association between genome conversion and seroconversion (1998)

Precore hepatitis B virus (HBV) mutants may gradually prevail during or after seroconversion (SC) from hepatitis B e antigen (HBeAg) to hepatitis B e antigen antibody (anti-HBe) status in many chronic hepatitis B (CH-B) patients. However, patients with CH-B still produce anti-HBe more than several years after SC, and the relationship between SC and genome conversion in the precore region has not been clarified. Therefore, in patients with CH-B who had a sustained loss of HBeAg and complete remission of hepatitis after SC, the precore region was sequenced in paired serum samples from 1 year before SC to 3 years after SC. Mutant precore defective HBV DNA was found in only 6 (19%) of 31 CH-B patients who had a complete remission of hepatitis after SC. Mixed-type HBV DNA (precore wild-type and mutant-type) was found in 4 (13%) patients. Wild-type HBV DNA was found in 21 (68%) CH-B patients after SC. Longer-term follow-up of 11 CH-B patients indicated that 3 of 11 patients experienced precore genome conversion 2 to 3 years after SC. E-plus DNA or e-minus DNA was semiquantitated by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) assays before and after SC. E-plus DNA levels decreased from 10(5.56+/-1.58) to 10(2.45+/-1.61). Similarly, e-minus DNA levels declined from 10(4.25+/-1.56) to 10(1.86+/-1.37). By dot-blot assay, serum HBV DNA became negative soon after SC, as did serum HBeAg. In contrast, HBeAg-containing immune complexes were still detected after SC. Anti-HBe antibody was produced throughout SC and thereafter, as determined by a sensitive experimental assay. Therefore, we conclude that genome-conversion in the precore region is a separate event from HBeAg/anti-HBe seroconversion.
Segments containing alternating purine and pyrimidine dinucleotides: patterns of polymorphism in humans and prevalence throughout phylogeny (1991)

Tandem dinucleotide repeats of GT or AC [(GT)n/(AC)n] where n greater than or equal to 14 are highly polymorphic and other simple repeats such as (CT)n/(AG)n and (A)n(T)n are also polymorphic. The uniformity of these sequences precludes a mechanistic differentiation between recombination or polymerase slippage. Since (GT)n/(AC)n or (CT)n/(AG)n segments of desired size were not available in our gene of interest, we analyzed a 187+ bp segment in the factor IX gene with multiple short dinucleotide repeats. This sequence contains a melody of short dinucleotide repeats which includes a 142+ bp segment of alternating purines and pyrimidines. Amplification of this sequence in 167 individuals of different ethnicity and direct sequencing of 106 individuals (23 kb of sequence) failed to reveal simple variation in the number of tandem dinucleotide repeats. However, polymorphism in the 142+ alternating purine and pyrimidine segment (RY)n was detected due to the insertion of two related repeat units of 24 bp (A) and 26 bp (B). Two previously described alleles (AB, A2B2) and two novel presumptive recombinants were found (A2B, A3B2) for a total of four alleles. An analysis of (RY)n segments in GenBank revealed an extraordinary enrichment in the genome of mammals, invertebrates, and yeast and a marked reduction in bacteria. Rodent (RY)n were larger and substantially more frequent than those in primates. When a second (RY)n was examined in the exon 8 of human factor IX gene, it was polymorphic at short repeats of (GT)n/(AC)n (n = 3-6) in Western Europeans and Koreans. In addition, an (RY)n in the dystrophin gene had four polymorphic alleles involving AT and GT dinucleotides. Thus (RY)n segments appear to be abundant and highly polymorphic. The asymmetric patterns of polymorphism and the absence of simple dinucleotide variation in 23 kb of sequence are compatible with recombination or sister chromatid exchange, but not polymerase slippage. By inference, recombination should underlie the polymorphisms at (GT)n/(AC)n since they are a subset of (RY)n and they commonly occur in the context of longer (RY)n.