gebhart/patrepeval huggingface dataset:
LC detectors: evaluation and practical implications of linearity (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLC DETECTORS: Evaluation and Practical Implications of LinearityCraig A. DorschelCite this: Anal. Chem. 1989, 61, 17, 951A–968APublication Date (Print):September 1, 1989Publication History Published online30 May 2012Published inissue 1 September 1989https://pubs.acs.org/doi/10.1021/ac00192a719https://doi.org/10.1021/ac00192a719research-articleACS PublicationsRequest reuse permissionsArticle Views124Altmetric-Citations25LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
DNA Electrochemistry: Charge-Transport Pathways through DNA Films on Gold (2021)

Over the past 25 years, collective evidence has demonstrated that the DNA base-pair stack serves as a medium for charge transport chemistry in solution and on DNA-modified gold surfaces. Since this charge transport depends sensitively upon the integrity of the DNA base pair stack, perturbations in base stacking, as may occur with DNA base mismatches, lesions, and protein binding, interrupt DNA charge transport (DNA CT). This sensitivity has led to the development of powerful DNA electrochemical sensors. Given the utility of DNA electrochemistry for sensing and in response to recent literature, we describe critical protocols and characterizations necessary for performing DNA-mediated electrochemistry. We demonstrate DNA electrochemistry with a fully AT DNA sequence using a thiolated preformed DNA duplex and distinguish this DNA-mediated chemistry from that of electrochemistry of largely single-stranded DNA adsorbed to the surface. We also demonstrate the dependence of DNA CT on a fully stacked duplex. An increase in the percentage of mismatches within the DNA monolayer leads to a linear decrease in current flow for a DNA-bound intercalator, where the reaction is DNA-mediated; in contrast, for ruthenium hexammine, which binds electrostatically to DNA and the redox chemistry is not DNA-mediated, there is no effect on current flow with mismatches. We find that, with DNA as a well hybridized duplex, upon assembly, a DNA-mediated pathway facilitates the electron transfer between a well coupled redox probe and the gold surface. Overall, this report highlights critical points to be emphasized when utilizing DNA electrochemistry and offers explanations and controls for analyzing confounding results.
Image-processing procedures for quality estimation in production of elastic foamy materials (2011)

We present a system for estimating the quality of polyurethane foam, which is able to measure the apparent density and mean size of the cell. The system consists of a flatbed scanner of high resolution, an electronic balance, and a personal computer. We present the new way for measuring the sample volume using six nonorthogonal projections and adapt the marker dividing line algorithm for cell segmentation in the scanned image. We examine an approach for determining the efficiency of the image-processing system operating as a measuring system on the basis of the Six Sigma method.
Synthesis of New Ruthenium(II) Carbonyl Hydrido, Alkenyl, and Alkynyl Complexes with Chelating Diphosphines (1994)

Substitution of one or two triphenylphosphine ligands of Ru(CO)ClH(PPh3)3 by bidentate diphosphines Ph2P(CH2)nPPh2 (L-L) (n = 1, dppm; n = 2, dppe; n = 3, dppp; n = 4, dppb) or 1,1′-bis(diphenylphosphino)ferrocene (dppf) led to hydrides Ru(CO)ClH(PPh3)2(L-L) or Ru-(CO)ClH(PPh3)(L-L). The hydrido complexes were characterized spectroscopically and by one X-ray structure. Hydride Ru(CO)ClH(PPh3)(dppf) crystallizes in the monoclinic space group P21/n, with a = 17.768(1) Å, b = 25.252(2) Å, c = 11.213(1) Å, β = 92.83(1)°, Z = 4, and V = 4459.3(6) Å3. Reaction of Ru(CO)ClH(PPh3)3 or [Ru(CO)H(MeCN)2(PPh3)2]+PF6-with 2 equiv of diphosphines L-L led to [Ru(CO)H(L-L)2]+A- (L-L = dppm, dppe, dppp) (A = Cl, PF6). Hydrides Ru(CO)ClH(PPh3)2(L-L) react with 1-alkynes to give alkenyl complexes Ru(CO)Cl(CH=CHR)(PPh3)(L-L) with a chelating diphosphine ligand. Ru(CO)ClH(PPh3)-(L-L) gave σ-alkynyl complexes Ru(CO)Cl(C≡CR)L(L-L) directly in their reactions with 1-alkynes. The hydride with dppb as the ligand showed the highest reactivity. The preparation of hexacoordinated alkenyl derivatives Ru(CO)Cl(CH=CHR)(PPh3)(L-L) with a chelating diphosphine was carried out by treatment of other alkenyl derivatives with the diphosphines. Surprisingly, reaction of alkenyls Ru(CO)Cl(CH=CHR)(Me2Hpz)(PPh3)2 (R = CMe3, P-MeC6H4) with dppf led to complexes Ru(CO)Cl(CH=CHR)(Me2Hpz)(dppf) by substitution of both PPh3 ligands instead of the dimethylpyrazole. Ruthenium alkenyls Ru-(CO)Cl(CH=CHR)L(dppf) (L = Me2Hpz, PPh3) react cleanly with 1-alkynes at room temperature to give alkynyl complexes Ru(CO)Cl(C=CR)L(dppf) in good yield. This reaction was applied to the synthesis of a bimetallic complex Ru(CO)Cl(PPh3)(dppf)(C≡C-p-C6H 4C≡C)-Ru(CO)Cl(PPh)(dppf) as a mixture of meso and dl diastereomers. © 1994 American Chemical Society.
Proteasome-Mediated Degradation of the Coactivator p300 Impairs Cardiac Transcription (2000)

The transcription of tissue-specific genes is controlled by regulatory factors and cofactors and is suppressed in cardiac cells by the antineoplastic agent doxorubicin. Here we show that exposure of cultured cardiomyocytes to doxorubicin resulted in the rapid depletion of transcripts for MEF2C, dHAND, and NKX2.5, three pivotal regulators of cardiac gene expression. Delivery of exogenous p300, a coactivator of MEF2C and NKX2.5 in cardiomyocytes, restored cardiac transcription despite the presence of doxorubicin. Furthermore, p300 also restored the accumulation of transcripts for MEF2C itself. Importantly, cardiocytes exposed to doxorubicin displayed reduced levels of p300 proteins. This was not due to alterations in the level of p300 transcripts; rather, and surprisingly, doxorubicin promoted selective degradation of p300 mediated by the 26S-proteasome machinery. Doxorubicin had no effect on the general level of ubiquitinated proteins or on the levels of beta-catenin, a protein known to be degraded by proteasome-mediated degradation. These results provide evidence for a new mechanism of transcriptional repression caused by doxorubicin in which the selective degradation of p300 results in reduced p300-dependent transcription, including production of MEF2C mRNA.
Dynamic Congestion Pricing for Multi-Class and Multi-Modes Transportation Systems with Asymmetric Cost Functions (2009)

This paper considers the problem of dynamic congestion pricing to determine optimal time-varying tolls for a queuing network with asymmetric cost functions. Through the combined applications of the Space-Time Expanded Network (STEN) and the conventional network equilibrium modeling techniques, we develop a multi-class, multi-mode and multi-criteria traffic network equilibrium model in which travelers of different classes have distinctive VOTs, and travelers from a same class perceive their travel disutility or generalized cost on a route as different weights of travel time and travel cost. Moreover, symmetric cost function model is extended to deal with the interactions between buses and private cars. In this paper we find that there exists uniform (anonymous) link toll pattern which can drive a multi-class, multi-modes and multi-criteria user equilibrium flow pattern to a system optimum when the system's objective function is measured in money.
Low-dose budesonide for maintenance of clinical remission in collagenous colitis: a randomised, placebo-controlled, 12-month trial (2014)

http://www.clinicaltrials.gov (NCT01278082) and http://www.clinicaltrialsregister.eu (EudraCT: 2007-001315-31).
The Universal Decompositional Semantics Dataset and Decomp Toolkit (2019)

We present the Universal Decompositional Semantics (UDS) dataset (v1.0), which is bundled with the Decomp toolkit (v0.1). UDS1.0 unifies five high-quality, decompositional semantics-aligned annotation sets within a single semantic graph specification---with graph structures defined by the predicative patterns produced by the PredPatt tool and real-valued node and edge attributes constructed using sophisticated normalization procedures. The Decomp toolkit provides a suite of Python 3 tools for querying UDS graphs using SPARQL. Both UDS1.0 and Decomp0.1 are publicly available at http://decomp.io.
Presentation, diagnosis and treatment of angioedema without wheals: a retrospective analysis of a cohort of 1058 patients (2014)

Our findings in this cohort of patients with angioedema provide new information on the clinical characteristics, diagnosis and treatment of this disease.
System Efficiency Issues for Natural Gas Fueled HCCI Engines in Heavy-Duty Stationary Applications (2002)

&lt;div class="htmlview paragraph"&gt;Homogeneous Charge Compression Ignition (HCCI) has been proposed for natural gas engines in heavy duty stationary power generation applications. A number of researchers have demonstrated, through simulation and experiment, the feasibility of obtaining high gross indicated thermal efficiencies and very low NO&lt;sub&gt;x&lt;/sub&gt; emissions at reasonable load levels. With a goal of eventual commercialization of these engines, this paper sets forth some of the primary challenges in obtaining high brake thermal efficiency from production feasible engines. Experimental results, in conjunction with simulation and analysis, are used to compare HCCI operation with traditional lean burn spark ignition performance. Current HCCI technology is characterized by low power density, very dilute mixtures, and low combustion efficiency. The quantitative adverse effect of each of these traits is demonstrated with respect to the brake thermal efficiency that can be expected in real world applications. While very low NO&lt;sub&gt;x&lt;/sub&gt; emissions are certainly achievable, significant technological improvements are still required to make HCCI engines competitive in the stationary power generation market.&lt;/div&gt;
[Uses of MTA, a review. Part 2: Clinical applications]. (2004)

Mineral trioxide aggregate (MTA) has been used in dentistry for the last five to eight years. Because of its high biocompatibility, its good sealing ability, and the fact that cemental tissues grow on this material, it has a relatively wide range of applications in endodontics. MTA may be used to cap exposed vital pulps, to seal open apices or perforations, or as a retro-filling material in apical surgery. These applications are presented in the current article, and discussed based on case reports.
Angelman's Syndrome in Infancy (1990)

The authors report an 11-month-old patient with the clinical features of Angelman syndrome and a 15q11 x 2-12 chromosomal deletion, thus demonstrating that the clinical features may be present in infancy and so allow early diagnosis. The features included pronounced postnatal growth failure, delayed dentition and ossification of growth centers. Low amniotic fluid alpha-fetoprotein was noted at 16 weeks of gestation. Head MRI showed only generalized atrophy. Some affected patients have a genetic marker similar to the chromosomal deletion associated with Prader-Willi syndrome. The importance of awareness of the clinical symptoms of Angelman syndrome is discussed.
Secondary myelodysplastic syndrome in multiple myeloma--a study of nine patients with an attempt to detect myeloma patients at risk. (1994)

Over a follow-up period of ten years, nine of our 100 patients with multiple myeloma (MM), developed myelodysplastic syndrome (MDS, preleukaemia). MDS occurred 19-156 (median 35) months from the diagnosis of MM. Six patients presented with pancytopenia and no patients had active MM at the time of MDS diagnosis. Three patients were defined as having refractory anaemia (RA) and six as refractory anaemia with excess blasts (RAEB) or RAEB in transformation (RAEBT), according to the FAB classification. The clinical course is characterized by increasing red blood cell and platelet transfusion requirements, recurrent infections and bleeding episodes. All patients, except for one, died within 3 to 8 (median 5) months from MDS diagnosis. The causes of death were sepsis or bleeding; three patients underwent leukaemic transformation. Thus, the clinical course of this small group of myeloma patients who developed secondary MDS (sMDS), was similar to other series of patients with sMDS. Serial bone marrow examinations suggest an initial hypercellular phase, followed by a rapidly evolving preterminal hypocellular marrow. In an attempt to detect MM patients at risk of developing sMDS, the epidemiological (including ethnic), clinical and laboratory data of the 9 MDS patients at the time of the MM presentation were reviewed and compared to the other MM patients. No significant differences were observed between the two groups in most parameters, except for two. All MDS patients were Ashkenazi Jews and no patients of Sepharadic origin developed MDS. Also, no IgA-myeloma patient developed MDS. If these findings are confirmed in a larger series, it may point to subgroups at risk which may require a different approach.(ABSTRACT TRUNCATED AT 250 WORDS)
Toward a better understanding of nonoccupational sound exposures and associated health impacts: Methods of the Apple Hearing Study (2022)

Globally, noise exposure from occupational and nonoccupational sources is common, and, as a result, noise-induced hearing loss affects tens of millions of people. Occupational noise exposures have been studied and regulated for decades, but nonoccupational sound exposures are not well understood. The nationwide Apple Hearing Study, launched using the Apple research app in November 2019 (Apple Inc., Cupertino, CA), is characterizing the levels at which participants listen to headphone audio content, as well as their listening habits. This paper describes the methods of the study, which collects data from several types of hearing tests and uses the Apple Watch noise app to measure environmental sound levels and cardiovascular metrics. Participants, all of whom have consented to participate and share their data, have already contributed nearly 300 × 10<sup>6</sup> h of sound measurements and 200 000 hearing assessments. The preliminary results indicate that environmental sound levels have been higher, on average, than headphone audio, about 10% of the participants have a diagnosed hearing loss, and nearly 20% of the participants have hearing difficulty. The study's analyses will promote understanding of the overall exposures to sound and associated impacts on hearing and cardiovascular health. This study also demonstrates the feasibility of collecting clinically relevant exposure and health data outside of traditional research settings.
Development of a Balanced Decoupling Unit for a Safe Automated Screwing Process during Human-Robot-Cooperation (2018)

The paper presents an automated screwing application inside a robot cell with human-robot-cooperation. It describes the use case and how the design challenges for process reliability and safety have been addressed from concept idea to the real set up. A focus is thereby set on the safety implementation, which is enabled by the development of a balanced decoupling unit, which enables force limiting at the end-effector during physical contact. The decoupling unit is implemented for the application with the screwing tool and is designed according to functional safety requirements. Its usability is validated in appropriate force measurements (according to ISO/TS 15066) and evaluated on its performance characteristics for the productivity of the robot application.
Fine-grained 3-D integrated circuit fabric using vertical nanowires (2015)

Continuous scaling of CMOS to sub-20nm technologies is proving to be challenging as MOSFETs are reaching fundamental limits and interconnection bottleneck is dominating IC power and performance. Migrating to fine-grained 3-D, to advance scaling, has been elusive due to incompatibility of CMOS in 3-D. We propose a new 3-D IC fabric, called Skybridge that addresses device, circuit, connectivity, heat management and manufacturing requirements in integrated 3D compatible manner. Our bottom-up evaluations accounting for material structures, manufacturing process, device, and circuit parasitics, reveal 60.5x density, and 16.5x performance/Watts benefits compared to CMOS for a 16-bit CLA. Experimental demonstration of the core device concept and key manufacturing steps mitigate technology risks.
A34 EFFECT OF AUTOPHAGY INDUCTION VIA TRPML1 ACTIVATION ON HEPATIC STEATOSIS (2019)

Extended criteria donors (ECD) are increasingly used to minimize the gap between available organs and liver transplant candidates. Steatotic liver grafts are a common type of ECD organs though they are often discarded as they are associated with poor transplant outcomes. Autophagy regulates lipid droplet (LD) accumulation in hepatocytes suggesting that altered autophagic function could be involved in the development of steatotic liver diseases. A lysosomal calcium channel, TRPML1, regulates autophagy via downstream TFEB activation and successive upregulation of autophagic and lysosomal gene expression. Therefore, we hypothesized that activation of TRPML1 via synthetic agonist ML-SA1 will attenuate hepatic steatosis by increasing autophagic flux and lysosomal function. The aims of the present study are to determine if:1)autophagic dysfunction contributes to steatosis, and 2)induction of autophagy via TRPML1 activation reduces hepatic steatosis in vitro. We established an in vitro fatty liver model by incubating a human hepatoma cell line (Hep3B cells) or isolated primary mouse hepatocytes with free fatty acids (FFA). LD accumulation was observed by BODIPY staining. To determine if autophagic flux was disrupted, Western blot (WB) for autophagy marker LC3II was performed in the presence of autophagy inhibitor, bafilomycin. To determine if TRPML1 was present and functional in Hep3B cells, cells were incubated with a bacterial toxin (VacA) that inhibits TRPML1 followed by incubation with ML-SA1. Cells were stained with LAMP1 and LC3II antibodies to visualize lysosomal and autophagy markers, respectively. WB was performed to measure levels of autophagy marker LC3II. FFA treatment in Hep3B cells induced significant LD accumulation as assessed by BODIPY staining. WB for LC3II with bafilomycin showed that a 1mM FFA concentration activates autophagy, whereas a 1.5mM FFA concentration and above disrupts autophagy. Hep3B cells incubated with the bacterial toxin VacA, displayed features consistent with disrupted TRPML1 function including enlarged LAMP1-positive vacuoles and accumulation of the autophagy marker LC3II. ML-SA1 treatment reversed vacuolation and normalized LC3II levels. These findings suggest that in a hepatocyte cell line TRPML1 activation can restore lysosomal function and autophagic flux in toxin-treated cells. We determined that in an in vitro model of hepatic steatosis, progressive accumulation of lipids is associated with disrupted autophagic flux. Furthermore, since TRPML1 is functional in a hepatocyte cell line and can be activated by the agonist ML-SA1, we can determine if ML-SA1 decreases steatosis. These studies will determine if TRPML1 could be a potential therapeutic target for reducing steatosis in ex vivo graft perfusion models to improve fatty liver grafts. CCC
Increased <i>SKP2</i> and <i>CKS1</i> Gene Expression Contributes to the Progression of Human Urothelial Carcinoma (2007)

Our results suggest that SKP2 has a major role in the regulation of p27 degradation and CKS1 has a supporting role for SKP2 function in human urothelial carcinoma.
A feature-based affine registration method for capturing background lung tissue deformation for ground glass nodule tracking (2021)

Lung nodule tracking assessment relies on cross-sectional measurements of the largest lesion profile depicted in initial and follow-up computed tomography (CT) images. However, apparent changes in nodule size assessed via simple image-based measurements may also be compromised by the effect of the background lung tissue deformation on the GGN between the initial and follow-up images, leading to erroneous conclusions about nodule changes due to disease. To compensate for the lung deformation and enable consistent nodule tracking, here we propose a feature-based affine registration method and study its performance vis-a-vis several other registration methods. We implement and test each registration method using both a lung- and a lesion-centered region of interest on ten patient CT datasets featuring twelve nodules, including both benign and malignant GGO lesions containing pure GGNs, part-solid, or solid nodules. We evaluate each registration method according to the target registration error (TRE) computed across 30 - 50 homologous fiducial landmarks surrounding the lesions and selected by expert radiologists in both the initial and follow-up patient CT images. Our results show that the proposed feature-based affine lesion-centered registration yielded a 1.1 ± 1.2 mm TRE, while a Symmetric Normalization deformable registration yielded a 1.2 ± 1.2 mm TRE, and a least-square fit registration of the 30-50 validation fiducial landmark set yielded a 1.5 ± 1.2 mm TRE. Although the deformable registration yielded a slightly higher registration accuracy than the feature-based affine registration, it is significantly more computationally efficient, eliminates the need for ambiguous segmentation of GGNs featuring ill-defined borders, and reduces the susceptibility of artificial deformations introduced by the deformable registration, which may lead to increased similarity between the registered initial and follow-up images, over-compensating for the background lung tissue deformation, and, in turn, compromising the true disease-induced nodule change assessment. We also assessed the registration qualitatively, by visual inspection of the subtraction images, and conducted a pilot pre-clinical study that showed the proposed feature-based lesion-centered affine registration effectively compensates for the background lung tissue deformation between the initial and follow-up images and also serves as a reliable baseline registration method prior to assessing lung nodule changes due to disease.