gebhart/patrepeval huggingface dataset:
Quimica e atividade biologica de derivados de 1,3-Diidro-2h-indol-3-ona (1986)

Chromobacterium violaceum produz o composto 3-[1,2-diidro-5-(5-hidroxi-1H-indol-3-il)-2-oxo-3H-pirrol-3-ilidenol-1,3- diidro-2H-indol-2-ona (pigmento I) Realizou--se um estudo das condicoes de cultura para otimizar a producao de pigmento I, como variacoes de nutrientes, temperatura e pH do meio foram correlacionados com o crescimento celular. Os cultivos feitos a 28°C durante 24 h de incubacao a pH 7, em meio liquido na ausencia de luz apresentou os melhores rendimentos. O pigmento I foi isolado de um extrato acetonico da bacteria, e identificado mediante dados espectroscopicos e constantes fisicas. A partir do pigmento I foram preparados os seguintes derivados:pigmento I-bromado, formilado,acetilado e sal de sodio. Estes derivados foram purificados por cromatografia de camada preparativa e identificados por UV, IV,H NMR, EM. Resultados preliminares de atividade biologica destes compostos mostraram ser antitripanossomais. Por envelhecimento da cepa nativa obtem-se uma cepa de cor branca que sintetiza um composto que foi identificado parcialmente como um derivado biftalato. Quando a cepa nativa foi irradiada a l >254 nm na faixa de fluencia entre 25,9 KJ/me 38,9 KJ/m, obtem-se uma bacteria mutante de cor vermelha- O composto isolado desta mutante foi identificado como isatina.
Abstr
Cloud and Edge Computing (2019)

This issue of the Series presents two articles in the area of cloud and edge computing. The first article is an exemplary of the IoT ecosystem that receives momentum for use in smart cities, public safety, and e-health. Fabio Giust et al. present a multi-access edge computing (MEC) architectural solution that allows for seamlessly integrating existing and future IoT platforms. The authors additionally present an IoT gateway middleware that enables running low-latency and/ or computationally intensive applications on generalized MEC-based systems.
Complex symbiosis involving within species variation in the response of Dictyostelium amoebae to Burkholderia bacteria [DATA] (2019)

http://creativecommons.org/licenses/by/4.0/
Carbon nanotube fibers doped with iron via Fenton reaction (2018)

In a wildly spreading research on carbon nanotube fibers as a potential material for the future, one of the most promising fields are electrical and electronic engineering. As it was mentioned repeatedly the main thing that need to be dealt with for a serious consideration of carbon nanotube structures in application as good conducting wires is a necessity of improvement their electrical conductivity values. In the many possibilities of such electrical properties improve, one of the best is chemical doping. In this work we present a oxidative doping treatment on carbon nanotube fibers via Fenton reaction. However the first assumptions on introduction hydroxide ion doping has changed after performing experiments. The reaction resulted in iron doping on carbon nanotube fibers. Such a result most probably is associated with a great reactivity of carbon nanotube with iron particles. This reactivity is being used in carbon nanotube structures production procedure, due to catalytic action of iron in the issue of carbon nanotube synthesis. This result, however differs from intentions gave us new carbon nanotube-iron composite, which seems to have a great potential for further research.
Plasma IL-6 levels following corticosteroid therapy as an indicator of ICU length of stay in critically ill COVID-19 patients (2021)

Intensive care unit (ICU) admissions and mortality in severe COVID-19 patients are driven by "cytokine storms" and acute respiratory distress syndrome (ARDS). Interim clinical trial results suggest that the corticosteroid dexamethasone displays better 28-day survival in severe COVID-19 patients requiring ventilation or oxygen. In this study, 10 out of 16 patients (62.5%) that had an average plasma IL-6 value over 10 pg/mL post administration of corticosteroids also had worse outcomes (i.e., ICU stay >15 days or death), compared to 8 out of 41 patients (19.5%) who did not receive corticosteroids (p-value = 0.0024). Given this potential association between post-corticosteroid IL-6 levels and COVID-19 severity, we hypothesized that the glucocorticoid receptor (GR or NR3C1) may be coupled to IL-6 expression in specific cell types that govern cytokine release syndrome (CRS). Examining single-cell RNA-seq data from BALF of severe COVID-19 patients and nearly 2 million cells from a pan-tissue scan shows that alveolar macrophages, smooth muscle cells, and endothelial cells co-express NR3C1 and IL-6, motivating future studies on the links between the regulation of NR3C1 function and IL-6 levels.
Central Auditory Processing Disorder in School-Aged Children (1998)

The rationale to evaluate for central auditory processing disorder (CAPD) in school-aged children is based on the assumption that an auditory-specific perceptual deficit underlies many learning problems including specific reading and language disabilities. A fundamental issue in this area is whether convincing empirical evidence exists to validate this proposition. Herein, we consider the issue of modality specificity by examining the extent to which reading, language, and attention disorders in school-aged children involve perceptual dysfunctions limited to a single sensory modality. Difficulty in validating CAPD as a diagnostic label is due in large part to use of the unimodal inclusive framework, which has biased the diagnosis to favor sensitivity of test results over documenting the specificity of the deficit. Indeed, empirical research documenting modality-specific auditory-perceptual dysfunction in this population is scarce. Therefore, the existing literature on this topic has not clarified the "true" nature of the problem, and has left many questions about this disorder unanswered. It is argued that demonstrating modality specificity is one way to rule out supramodal disorders as explanations for observed dysfunction. Multimodal perceptual testing is one logical approach to help clarify this area of investigation.
Dislocation strain as the mechanism of phonon scattering at grain boundaries (2016)

Thermal conductivities of polycrystalline thermoelectric materials are satisfactorily calculated by replacing the commonly used Casimir model (freqeuncy-independent) with grain boundary dislocation strain model (frequency-dependent) of Klemens. It is demonstrated that the grain boundaries are better described as a collection of dislocations rather than perfectly scattering interfaces.
Engraftment in Nonobese Diabetic Severe Combined Immunodeficient Mice of Human CD34+ Cord Blood Cells After Ex Vivo Expansion: Evidence for the Amplification and Self-Renewal of Repopulating Stem Cells (1999)

Understanding the repopulating characteristics of human hematopoietic stem/progenitor cells is crucial for predicting their performance after transplant into patients receiving high-dose radiochemotherapy. We have previously reported that CD34+cord blood (CB) cells can be expanded in vitro for several months in serum containing culture conditions. The use of combinations of recombinant early acting growth factors and the absence of stroma was essential in determining this phenomenon. However, the effect of these manipulations on in vivo repopulating hematopoietic cells is not known. Recently, a new approach has been developed to establish an in vivo model for human primitive hematopoietic precursors by transplanting human hematopoietic cells into sublethally irradiated nonobese diabetic severe combined immunodeficient (NOD/SCID) mice. We have examined here the expansion of cells, CD34+ and CD34+38− subpopulations, colony-forming cells (CFC), long-term culture initiating cells (LTC-IC) and the maintenance or the expansion of SCID-repopulating cells (SRC) during stroma-free suspension cultures of human CD34+ CB cells for up to 12 weeks. Groups of sublethally irradiated NOD/SCID mice were injected with either 35,000, 20,000, and 10,000 unmanipulated CD34+ CB cells, which were cryopreserved at the start of cultures, or the cryopreserved cells expanded from 35,000, 20,000, or 10,000 CD34+ cells for 4, 8, and 12 weeks in the presence of a combination of early acting recombinant growth factors (flt 3/flk2 ligand [FL] + megakaryocyte growth and development factor [MGDF] ± stem cell factor [SCF] ± interleukin-6 [IL-6]). Mice that had been injected with ≥20,000 fresh or cryopreserved uncultured CD34+ cells did not show any sign or showed little engraftment in a limited number of animals. Conversely, cells that had been generated by the same number of initial CD34+ CB cells in 4 to 10 weeks of expansion cultures engrafted the vast majority of NOD/SCID mice. The level of engraftment, well above that usually observed when the same numbers of uncultured cells were injected in the same recipients (even in the presence of irradiated CD34− cells) suggested that primitive hematopoietic cells were maintained for up to 10 weeks of cultures. In addition, dilution experiments suggest that SRC are expanded more than 70-fold after 9 to 10 weeks of expansion. These results support and extend our previous findings that CD34+ CB stem cells (identified as LTC-IC) could indeed be grown and expanded in vitro for an extremely long period of time. Such information may be essential to design efficient stem cell expansion procedures for clinical use.
Refined Beam Models for Static and Dynamic Analysis of Wings and Rotor Blades (2010)

This paper presents the finite element analysis of slender thin-walled bodies by means of different finite element models. The beam formulation is given in the framework of the Carrera Unified Formulation, CUF, which considers the order of the theory, N, as a free parameter of the analysis. N is the order of the 1D displacement expansion. The displacement components are, in fact, expanded in terms of the cross-section coordinates, (x, z), by using a set of 1D generalized displacement variables. The refined kinematic models are based on Taylor-type polynomials. The finite element formulation is exploited in order to be able to face arbitrary cross-section geometries. FE's matrices are obtained in terms of a few fundamental nuclei which are formally independent of both N and the number of element nodes. A cubic (4 nodes) approximation along the beam axis, (y), is used. Structural analyses are conducted starting from classical beam theories, refined models are then introduced to evaluate non-classical effects. Aircraft wing and wind turbine blade models are analyzed. Static and dynamic analyses are conducted. It has mainly been concluded that the enhanced refined beam element, which has been formulated via CUF, is able to detect the so-called shell-like mechanical behaviors, that is, shell-like results can be obtained using higher-order beam elements. The shell-like capabilities include the detection of the local displacement field induced by a concentrated load, and natural modes characterized by the presence of waves along the cross-section contour.
Redox-flow batteries for high energy-to-power ratio storage: a preliminary experimental study (2021)

In conjunction with the worldwide interest in the safeguard of the environment, the possibility of storing electricity to allow larger renewable energy source penetration is increasingly getting ground. In this framework, among all the potential stationary energy storage systems, Redox Flow Batteries (RFBs) appear as one of the most promising solutions. This is because of their several advantages, such as the unique capability to decouple energy content from power, which makes them well adaptable at different network services and installation locations. This paper aims to display the RFBs scenario inside the global stationary storage situation. The principle of operation of these batteries is illustrated and the current landscape described. Some preliminary experimental results of an existing commercial solution of a vanadium RFB (VRFB) are presented, highlighting strengths and weaknesses points of this category. Charge and discharge experimental tests under realistic grid-interconnection scenario are performed, measuring the VRFB under study global efficiency, by an estimation of its internal losses: shunt currents, self-discharge rate and auxiliary system power consumption. Considering all these losses, the results display an overall VRFB efficiency around 50%, against the typical values taken from literature, that range between 70% to 90%.
Size-dependent bioactivity of electrosprayed core–shell chitosan-alginate particles for protein delivery (2022)

Nano-bio interactions are size-dependent. The present study investigates whether core-shell chitosan-alginate particle size governs biological activities as well as protein release profile. A coaxial electrospraying was used to fabricate bovine serum albumin (BSA)-loaded core-shell micro/nanoparticles and were fully characterized. The bio/hemocompatibility of the particles was assessed using MTT and hemolytic assays, respectively, followed by the uptake assessment using flow cytometry. Finally, protein absorption was investigated using SDS-PAGE. The SEM size of the microparticles, the hydrodynamic, and the actual sizes of the nanoparticles were 1.2 μm, 90.49 nm, and 50 nm, respectively. Interactions among two polymers and BSA were observed using DSC analysis. BET analysis showed a more surface area for nanoparticles. A sustained release trend of BSA was observed after 14- and 10-day for microparticles and nanoparticles, respectively. Microparticles exhibited excellent hemocompatibility (< 5% hemolysis) and cell viability (at least > 70%) in all concentrations. However, acceptable hemolytic activity and cell viability were observed for nanoparticles in concentrations below 250 μg/mL. Furthermore, nanoparticles showed greater cellular uptake (~ 4 folds) and protein absorption (~ 1.61 folds) than microparticles. Overall, the developed core-shell chitosan-alginate particles in the micro/nanoscale can be promising candidates for biomedical application and regenerative medicine regarding their effects on above mentioned biological activities.
Interactive Music on the Web (2019)

The web browser provides a novel platform for interactive music with a number of unique advantages: easy access, online collaboration, platform independence and abundant online resources. The goals of this chapter are (1) to help readers comprehend the benefits and challenges involved in developing and using browser-based interactive music applications; (2) to provide a basic technical foundation for writing a Web Audio program in the context of creating interactive music applications; and (3) to explore the opportunities that interactive music on the web offers, by identifying the five emerging trends of current music applications. Ultimately, we wish to encourage readers to create applications that permit novel musical expression and collaboration through the use of Web Audio.
Supplementary Figure 2 from Resistance to Selective BRAF Inhibition Can Be Mediated by Modest Upstream Pathway Activation (2023)

&lt;p&gt;PDF file - 455K&lt;/p&gt;
Biosynthesis of Novel Statins by Combining Heterologous Genes from <i>Xylaria</i> and <i>Aspergillus</i> (2018)

For many secondary metabolites, heterologous synthesis is the definitive step to determine their required biosynthetic genes. Using a multivector expression system in Saccharomyces cerevisiae, we reconstituted not only two natural statins from two fungal species, i.e., lovastatin from Aspergillus terreus and FR901512 from Xylaria grammica, but also new statin structures by mixing their genes. Combinatorial gene exchange experiments revealed the functional promiscuity of two polyketide synthases in A. terreus, lovB, and lovF; they could synthesize FR901512 with Xylaria genes. Key structure determinants of statins are essential accessory genes that are irreplaceable across species.
Effects of Chain Length Modification and Bis(ethyl) Substitution of Spermine Analogs on Purine−Purine−Pyrimidine Triplex DNA Stabilization, Aggregation, and Conformational Transitions (1997)

The natural polyamines--putrescine, spermidine, and spermine--are known to stabilize pyrimidine-purine-pyrimidine and purine-purine-pyrimidine triplex DNA formation. We studied the ability of two tetramine and two pentamine analogs of spermine and their bis(ethyl) derivatives to stabilize triplex DNA formation between 5'-TG3TG4TG4TG3T-3' and its target duplex probe, consisting of the oligonucleotides 5'-TCGAAG3AG4AG4AG3A-3' and 5'-TCGATC3TC4TC4TC3T-3'. We used electrophoretic mobility shift assay (EMSA), melting temperature (Tm) measurements, and circular dichroism (CD) spectroscopy to evaluate the effects of these novel polyamine analogs on triplex DNA stability, dissociation constants, aggregation, and conformation. In general, pentamines were more efficacious than tetramines in stabilizing triplex DNA, although most of the polyamines with pendant free amino groups caused DNA aggregation below 50% conversion to triplex DNA. Ethyl substitution of these pendant amino groups lowered their efficacy approximately 2-fold in stabilizing triplex DNA; however, this effect was more than compensated for by the lack of DNA aggregation in the presence of bis(ethyl)polyamines. A concentration-dependent increase in the Tm of triplex DNA was observed in the presence of polyamines. CD spectral measurements showed distinct differences in the conformation of triplex DNA stabilized in the presence of polyamines compared to the CD spectra of the oligonucleotides alone. Temperature-dependent CD spectra of triplex DNA showed monophasic melting in the absence and presence of polyamines, suggesting duplex/triplex --> single-stranded DNA transition. These results indicate that structural modifications of polyamines is an effective strategy to develop triplex DNA-stabilizing ligands, with potential applications in antigene therapeutics.
Pyrrolo[2,1‐<i>c</i>][1,4]benzodiazepines. Synthesis of new cyclic amidines and fused[<i>a</i>]triazolo, tetrazolo and oxadiazolo derivatives (1997)

Abstract We report the practical synthesis of new cyclic amidines from thiolactams and the preparation of fused[ a ]triazolo, tetrazolo and oxadiazolo derivatives via hydrazidines and oximes, in the pyrrolo[2,1‐ c ]‐[1,4]benzodiazepine series.
Shape Retrieval with Flat Contour Segments (2002)

Content-based image retrieval (CBIR) is an important issue in the computer vision community. Both visual and textual descriptions are employed when the user formulates his queries. Shape feature is one of the most important visual features. The shape feature is essential as it corresponds to the region of interest in images. Consequently, the shape representation is fundamental. The shape comparisons must be compact and accurate, and must own properties of invariance to several geometric transformations such as translation, rotation and scaling, though the representation itself may be
Smart Multifunctional Fluids for Lithium Ion Batteries: Enhanced Rate Performance and Intrinsic Mechanical Protection (2013)

Lithium ion batteries are attractive power sources for the consumer electronics market and are being aggressively developed for road transportation. Nevertheless, issues with safety and reliability need to be solved prior to the large-scale uptake of these batteries. There have recently been significant development and assessment of materials with resistance to mechanical abuse, with the aims of reinforcing the battery and preventing puncturing during a crash. Most of the work on battery mechanical safety has concentrated on the external packaging of batteries, with little attention being paid to the enclosed electrolyte. We report on smart multifunctional fluids that act as both highly conductive electrolytes and intrinsic mechanical protectors for lithium ion batteries. These fluids exhibit a shear thickening effect under pressure or impact and thus demonstrate excellent resistance to crushing. Also, the fluids show higher ionic conductivities and comparable redox stability windows to the commercial liquid electrolytes.
Synthesis, Self-Assembly, and Characterization of Supramolecular Polymers from Electroactive Dendron Rodcoil Molecules (2004)

We report here the synthesis and self-assembly of a series of three molecules with dendron rodcoil architecture that contain conjugated segments of oligo(thiophene), oligo(phenylene-vinylene), and oligo(phenylene). Despite their structural differences, all three molecules yield similar self-assembled structures. Electron and atomic force microscopy reveals the self-assembly of the molecules into high aspect ratio ribbon-like nanostructures which at low concentrations induce gelation in nonpolar solvent. Self-assembly results in a blue-shifted absorption spectrum and a red-shifted, quenched fluorescence spectrum, indicating aggregation of the conjugated segments within the ribbon-like structures. The assembly of these molecules into one-dimensional nanostructures is a route to pi-pi stacked supramolecular polymers for organic electronic functions. In the oligo(thiophene) derivative, self-assembly leads to a 3 orders of magnitude increase in the conductivity of iodine-doped films due to self-assembly. We also found that electric field alignment of these supramolecular assemblies can be used to create arrays of self-assembled nanowires on a device substrate.
Video-based system for satellite proximity operations (2002)

The servicing of satellites in space will extend their life and reduce operational costs. Servicing using an unmanned servicer spacecraft offers a reduction of launching cost when compared with that of manned spacecraft. However, the communication delay, intermittence and limited bandwidth between the ground and on-orbit segments renders direct teleoperated ground control infeasible. The servicer will therefore have to operate with a high degree of autonomy. The on-board sensor system should allow the servicer spacecraft to approach the target satellite, identify its unknown position and orientation from an arbitrary direction, and manoeuvre to align with a docking interface [5,7,10].
Characterization of Microfabricated Probes for Combined Atomic Force and High-Resolution Scanning Electrochemical Microscopy (2006)

A combined atomic force and scanning electrochemical microscope probe is presented. The probe is electrically insulated except at the very apex of the tip, which has a radius of curvature in the range of 10-15 nm. Steady-state cyclic voltammetry measurements for the reduction of Ru(NH3)6Cl3 and feedback experiments showed a distinct and reproducible response of the electrode. These experimental results agreed with finite element simulations for the corresponding diffusion process. Sequentially topographical and electrochemical studies of Pt lines deposited onto Si3N4 and spaced 100 nm apart (edge to edge) showed a lateral electrochemical resolution of 10 nm.
Supplementary Table 1 and 2 from Blood mRNA Expression Profiling Predicts Survival in Patients Treated with Tremelimumab (2023)

&lt;p&gt;Supplementary Table 1 and 2. Table S1 contains all of the genes tested. Table S2 describes patient selection.&lt;/p&gt;
Detection of dengue virus serotypes 1, 2 and 3 in selected regions of Kenya: 2011–2014 (2016)

From 2011-2014 serotypes 1, 2 and 3 were detected in the Northern and Coastal parts of Kenya. This confirmed the occurrence of cases and active circulation of dengue in parts of Kenya. These results have documented three circulating serotypes and highlight the need for the establishment of active dengue surveillance to continuously detect cases, circulating serotypes, and determine dengue fever disease burden in the country and region.