1116 GENITAL BLOODFLOW AND BLADDER FUNCTION IN A FEMALE RAT MODEL FOR NERVE-SPARING HYSTERECTOMY (2011)

You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Basic Research1 Apr 20111116 GENITAL BLOODFLOW AND BLADDER FUNCTION IN A FEMALE RAT MODEL FOR NERVE-SPARING HYSTERECTOMY Fabio Castiglione, Frank Strittmatter, Trinity J. Bivalacqua, Fabio Benigni, Andrea Russo, Patrizio Rigatti, Francesco Montorsi, and Petter Hedlund Fabio CastiglioneFabio Castiglione Milan, Italy More articles by this author , Frank StrittmatterFrank Strittmatter Milan, Italy More articles by this author , Trinity J. BivalacquaTrinity J. Bivalacqua Baltimore, MD More articles by this author , Fabio BenigniFabio Benigni Milan, Italy More articles by this author , Andrea RussoAndrea Russo Milan, Italy More articles by this author , Patrizio RigattiPatrizio Rigatti Milan, Italy More articles by this author , Francesco MontorsiFrancesco Montorsi Milan, Italy More articles by this author , and Petter HedlundPetter Hedlund Milan, Italy More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.713AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Radical hysterectomy (RH) may cause bladder and sexual dysfunctions. Autonomic nerve preservation in RH may improve late morbidity. Preclinical models to study nerve-sparing procedures are available for prostatectomy but not for RH. The aim of the current study was to establish and evaluate models for nerve-sparing RH in female rats. METHODS After ethical approval, female Sprague Dawley rats (250g) were subjected to unilateral pelvic nerve (PN) crush (3x30 sec. with a forceps; PNC; n=5) or crush of the PN and nerves from the major pelvic ganglion supplying the vagina and bladder (clock-nerve crush; CNC; n=5). During anesthesia, mean arterial blood pressure (MAP) was monitored and clitoral and vaginal bloodflows (laser Doppler; tissue perfusion units; TPU) were registered during stimulation (5V; 20Hz) of the PN. Bladder function was studied by cystometry at 3 and 10 days in conscious rats with bilateral PNC or CNC (each n=3) in comparison to sham operated rats (n=6). Values are mean±SEM. T-test was used for comparisons. RESULTS Baseline vaginal flows at 3 and 10 days were 7.6±2.2 and 10.9±0.9 for PNC and 7.9±1.8 and 11.6±1.1 TPU for CNC. Control stimulations of the intact PN at 3 and 10 days caused vaginal peak flows of all rats which were 0.22±0.04 and 0.26±0.02 TPU/MAP for PNC and 0.26±0.04 and 0.27±0.06 TPU/MAP for CNC. Stimulation of the PNC or CNC side yielded lower vaginal peak flows (p<0.05) which were 0.12±0.03 and 0.13±0.02 TPU/MAP for PNC and 0.10±0.03 and 0.14±0.05 TPU/MAP for CNC. Baseline clitoral flows at 3 and 10 days were 5.7±1.3 and 8.4±2.1 TPU for PNC and 5.8±1.3 and 7.7±1.3 TPU for CNC. At 3 days, control stimulations amounted to 0.15±0.05 and 0.12±0.09 TPU/MAP for PNC and CNC. Responses of the crush-side were 0.06±0.02 TPU/MAP for PNC and 0.06±0.02 TPU/MAP for CNC. At 10 days, responses were reduced (p<0.05) from 0.11±0.03 to 0.05±0.01 TPU/MAP for PNC. After CNC, responses were reduced (p<0.05) from 0.10±0.01 to 0.05±0.01 TPU/MAP. Whereas sham rats exhibited regular voiding patterns, rats with bilateral PNC or CNC did not exhibit regular micturitions but detrusor overactivity with non-voiding contractions, dribbling incontinence or atonic large bladder. CONCLUSIONS PNC and CNC procedures cause similar effects on genital bloodflow and bladder function in female rats and are proposed as relevant models for the study of nerve-sparing RH. Development of a novel animal model will forward our understanding of the detrimental effects of PN injury at the time of RH and advance therapeutic strategies of associated urogenital dysfunctions. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e448-e449 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Fabio Castiglione Milan, Italy More articles by this author Frank Strittmatter Milan, Italy More articles by this author Trinity J. Bivalacqua Baltimore, MD More articles by this author Fabio Benigni Milan, Italy More articles by this author Andrea Russo Milan, Italy More articles by this author Patrizio Rigatti Milan, Italy More articles by this author Francesco Montorsi Milan, Italy More articles by this author Petter Hedlund Milan, Italy More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...