gebhart/patrepeval huggingface dataset:
24-Epibrassinolide as a Modifier of Antioxidant Activities and Membrane Properties of Wheat Cells in Zearalenone Stress Conditions (2018)

The mechanism of action of brassinosteroids (BRs) in plant cells under stress has not been fully explained, despite ample evidence of their protective effects. The aim of this study was to investigate the significance of physicochemical properties of cell membranes during an interaction with BRs under stress conditions induced by a mycotoxin zearalenone (ZEA). Experiments were performed in in vitro cultures of wheat cells obtained from immature embryos of tolerant and sensitive genotypes. ZEA added to media (30 µM) accumulated in greater amounts in the cells of sensitive wheat, contrary to BRs, which accumulated in greater amounts by the tolerant genotype when added to media at 0.1 µM. Incorporation of 24-epibrassinolide (EBR) stimulated synthesis of casta- and homocastasterone, that is, endogenous BRs present in wheat cells, and enhanced the content of homocastasterone. When the cultures were supplemented with the mixture of ZEA and EBR, castasterone synthesis was stimulated to a higher degree in cells of the sensitive plant. EBR and ZEA added separately activated antioxidant enzyme systems in both genotypes but with preference for the sensitive one. In the cells treated with ZEA + EBR, the activation was close to that observed for EBR alone. The study discussed also the role of membrane permeability, electrokinetic potential changes, and structural properties of native (plasmalemma) and model (DOPC) monolayers in the mechanism of EBR-induced protection, including the possibility of replacing ZEA absorbed in the membrane lipid layers by BR molecules, independently of the activation of the antioxidant system.
Analysis of the effects of wall temperature swing on reciprocating internal combustion engine processes (2017)

A thermal wall temperature swing model was built to capture the transient effects of various material properties and coating layers on the intra-cycle wall temperature of an internal combustion engine. This model was used with a thermodynamic engine simulation to predict and analyze the effects of different types of in-cylinder insulation on engine performance. Coatings that allow the surface temperature to swing in response to the gas’ cyclical heat flux enable approximately 1/3 of the energy that was prevented from leaving the gas during expansion to be recovered while improving volumetric efficiency. Reductions in compression work due to better volumetric efficiency and less heat transfer from the walls to the gas accounted for half of the improvements, while additional work extraction during combustion and expansion accounted for the other half. As load increases, the temperature swing and benefits derived from it also increase. NSFC improvements of 0.5% to 1% were seen with a highly swinging coating in the throttled regime for a realistic engine geometry and coating area, up to 2.5% at high loads.
MINIMALLY INVASIVE PARATHYROIDECTOMY USING SURGEON‐PERFORMED ULTRASOUND AND SESTAMIBI (2007)

SPU in the hands of an experienced surgeon in association with sestamibi is a reliable tool for the preoperative localization of parathyroid adenomas and facilitates a minimally invasive procedure.
Experimental and theoretical analysis of efficient erbium-doped fiber power amplifiers (1991)

The efficiency of Er/sup 3+/-doped fiber power amplifiers (EDFAs) pumped at 980 nm was experimentally investigated and quantum conversion efficiencies (QCE) up to 0.89 were achieved. The experiment was accurately simulated by a computer model using only measured input parameters. The model was further used in an analysis of power amplifiers pumped at 980 and 1480 nm that included waveguide optimization and Er/sup 3+/ confinement. The QCE can be enhanced by increasing the numerical aperture (NA) and confining the Er/sup 3+/ ions to the central region of the core. At pump powers typically used for packaged EDFAs (25-100 mW). QCE can be improved by up to 60% by increasing the NA from 0.15 to 0.25, and confined Er/sup 3+/ doping can provide an improvement of up to 20%. However, NA and Er/sup 3+/ confinement have insignificant effects on the noise figure when both the cutoff wavelength and the fiber length are optimized with respect to QCE.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Theory and modelling of the interaction of two parallel supersonic plasma jets (1998)

The objective of the present work is to predict the profile of the shock front formed by the interaction of two parallel diverging supersonic plasma jets. The form of the front is determined by the divergence of the plasma jets and by the distance between their sources, but the maximum angle of the front inclination at the head point of the shock wave is determined by the plasma properties only. The streamlines of the two plasma jets are diverging before the front and are parallel behind it. The diffusion of the ions flying from two different sources was calculated numerically up to a distance of 10 times the source separation in the region bounded by two symmetric shock fronts. For an ion temperature of K, a jet velocity of , and initial densities of and (corresponding to a Mach number of 10 and Knudsen numbers of 0.04 and 0.4 respectively) the partial and total density distribution in the diffusion zone was found. The ion distribution is not homogeneous in all of investigated regions. The most homogeneous distribution was obtained with an initial density of . The shock model predictions were compared with a free expansion model, and the densities are radically different.
Data from An Autocrine VEGF/VEGFR2 and p38 Signaling Loop Confers Resistance to 4-Hydroxytamoxifen in MCF-7 Breast Cancer Cells (2023)

&lt;div&gt;Abstract&lt;p&gt;Tamoxifen, a partial estrogen receptor antagonist, is part of the standard treatment of both primary and advanced breast cancers. However, significant proportions of breast cancers are either &lt;i&gt;de novo&lt;/i&gt; resistant or develop tamoxifen resistance during the course of treatment through mechanisms which have been only partly characterized. We have previously found that high vascular endothelial growth factor (VEGF) or VEGF receptor 2 (VEGFR2) expression and concomitant high p38 mitogen-activated protein kinase activity within breast cancers predict a poor outcome for tamoxifen-treated patients. Here, we have molecularly dissected how VEGF/VEGFR2 and p38 are linked, and contribute to tamoxifen resistance within breast cancer using a MCF-7 BC cell model with different 4-hydroxytamoxifen (4-OHT) responsiveness. We report that MCF-7 breast cancer cell lines with tamoxifen resistance have increased secretion of VEGF and increased signaling through VEGFR2 compared with parental MCF-7 cells. 4-OHT treatment caused the ablation of VEGF secretion in parental MCF-7 cells, whereas in the tamoxifen-resistant subline, a VEGF/VEGFR2 signaling loop was still evident upon treatment. Increased basal levels of total and phosphorylated p38 were observed in tamoxifen-resistant cells. Pharmacologic inhibition of p38 reduced the proliferation of both tamoxifen-responsive and tamoxifen-resistant cells and showed an additive growth-inhibitory effect in combination with 4-OHT. A connection between VEGF/VEGFR2 and p38 signaling was identified by VEGF and VEGFR2 knockdown, which equally reduced both the total and the active forms of p38 in tamoxifen-resistant cells. Taken together, our results suggest that decreased sensitivity to 4-OHT is caused by a death-protecting VEGF/VEGFR2 and p38 growth factor loop in breast cancer cells. Inhibition of these signaling pathways may be beneficial to overcome tamoxifen resistance. (Mol Cancer Res 2008;6(10):1630–8)&lt;/p&gt;&lt;/div&gt;
Aggregation deaggregation influenced selective and sensitive detection of Cu<sup>2+</sup>and ATP by histidine functionalized water-soluble fluorescent perylene diimide under physiological conditions and in living cells (2015)

A novel PDI-HIS probe detects Cu<sup>2+</sup>to form aggregated nonfluorescent complex. Addition of 0.58 ppm ATP to this complex causes its rapid disaggregation thereby recovering the fluorescence by ∼99%<italic>in vitro</italic>and in A549 living cells.
Synthesis and Characterization of the Mixed‐Linker Copper(II) Coordination Polymer [Cu(HO<sub>3</sub>PC<sub>6</sub>H<sub>4</sub>SO<sub>3</sub>)(C<sub>10</sub>N<sub>2</sub>H<sub>8</sub>)]·H<sub>2</sub>O (2011)

Abstract A new copper(II) phosphonatobenzenesulfonate incorporating 4,4′‐bipyridine (4,4′‐ bipy ) as auxiliary ligand has been discovered through systematic high‐throughput (HT) screening of the system Cu(NO 3 ) 2 ·3H 2 O/H 2 O 3 PC 6 H 4 SO 3 H/4,4′‐ bipy using different solvents. The hydrothermal synthesis of [Cu(HO 3 PC 6 H 4 SO 3 )(C 10 H 8 N 2 )]·H 2 O ( 1 ) was further optimized by screening various copper(II) salts. The crystal structure of 1 was determined by single‐crystal X‐ray diffraction and unveiled the presence of isolated sixfold coordinated Jahn–Teller‐distorted Cu 2+ ions. The isolated CuN 2 O 4 octahedra are interconnected by phosphonate and sulfonate groups to form chains along the c‐ axis. The organic groups, namely phenyl rings and 4,4′‐ bipy molecules cross‐link the chains into a three‐dimensional framework. Water molecules are found in the narrow voids in the structure which are held by weak hydrogen bonds. Upon dehydration, the structure of 1 undergoes a phase transition, which was confirmed by TG measurements and temperature dependent X‐ray powder diffraction. The new structure of 1‐h was refined with Rietveld methods. Detailed inspection of the structure revealed the directional switching of the Jahn–Teller distortion upon de/rehydration. Weak ferro‐/ferrimagnetic interactions were observed by magnetic investigations of 1 , which switch to antiferromagnetic below 3.5 K. Compounds 1 and 1‐h are further characterized by thermogravimetric and elemental analysis as well as IR spectroscopy.
Human skin organ culture for assessment of chemically induced skin damage (2012)

The move away from animal models for skin safety testing is inevitable. It is a question of when, not if. As skin safety studies move away from traditional animal-based approaches, a number of replacement technologies are becoming available. Human skin in organ culture is one such technology. Organ-cultured skin has several features that distinguish it from other technologies. First and foremost, organ-cultured skin is real skin. Almost by definition, therefore, it approximates the intact skin better than other alternative models. Organ culture is an easy-to-use and relatively inexpensive approach to preclinical safety assessment. Although organ culture is not likely to replace high-throughput enzyme assays or monolayer culture/skin equivalent cultures for initial compound assessment, organ culture should find use when the list of compounds to be evaluated is small and when simpler models have narrowed the dose range. Organ-cultured skin also provides a platform for mechanistic studies.
Response to the Waddell et al. Letter (2009)

ADVERTISEMENT RETURN TO ISSUEPREVLetters to the Edito...Letters to the EditorNEXTResponse to the Waddell et al. LetterGeorge S. Bailey*, Gayle A. Orner, Clifford B. Pereira, and James A. SwenbergView Author Information Linus Pauling InstituteOregon State University Linus Pauling InstituteOregon State University Department of StatisticsOregon State University University of North Carolina at Chapel HillCite this: Chem. Res. Toxicol. 2009, 22, 9, 1493–1494Publication Date (Web):August 20, 2009Publication History Received4 August 2009Published online20 August 2009Published inissue 21 September 2009https://pubs.acs.org/doi/10.1021/tx900269jhttps://doi.org/10.1021/tx900269jletterACS PublicationsCopyright © 2009 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views483Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (40 KB) Get e-AlertscloseSUBJECTS:Cancer,Testing and assessment,Toxicology,Tumors Get e-Alerts
Hypertrophy and physiological death of equine chondrocytes <i>in vitro</i> (2007)

This culture system should greatly assist laboratory studies aimed at elucidating the pathogenesis of osteochondrosis.
Efficient Fabrication Process of Metal Nanodot Arrays Using Direct Nanoimprinting Method with a Polymer Mold (2015)

A new fabrication process of metal nanodot arrays using the thermal dewetting method was developed in this study. This process was comprised of three steps: thin Au film deposition on a quartz glass substrate, groove patterning by direct nanoimprinting, and self-organization of metal nanodot arrays by thermal dewetting. A new idea to utilize a polymer film mold for groove patterning by direct nanoimprinting was examined. The polymer film mold was prepared by hot-embossing groove patterns of a mother mold on a cyclo olefin polymer (COP) film. The mother mold was prepared from a silicon wafer. The polymer film mold was used for direct nanoimprinting on a metal film deposited on a quartz substrate. The experimental results revealed that the COP film mold can effectively form a micro groove pattern on the Au film despite the COP film mold being softer than the Au film. The micro groove on the Au film was also found to be effective in aligning the nanodots in lines. The micro groove patterning using the COP film mold was also confirmed to be useful in controlling the dot size and alignment during the thermal dewetting process.
Differential Roles of Insulin and IGF-1 Receptors in Adipose Tissue Development and Function (2016)

To determine the roles of insulin and insulin-like growth factor 1 (IGF-1) action in adipose tissue, we created mice lacking the insulin receptor (IR), IGF-1 receptor (IGF1R), or both using Cre-recombinase driven by the adiponectin promoter. Mice lacking IGF1R only (F-IGFRKO) had a ∼25% reduction in white adipose tissue (WAT) and brown adipose tissue (BAT), whereas mice lacking both IR and IGF1R (F-IR/IGFRKO) showed an almost complete absence of WAT and BAT. Interestingly, mice lacking only the IR (F-IRKO) had a 95% reduction in WAT, but a paradoxical 50% increase in BAT with accumulation of large unilocular lipid droplets. Both F-IRKO and F-IR/IGFRKO mice were unable to maintain body temperature in the cold and developed severe diabetes, ectopic lipid accumulation in liver and muscle, and pancreatic islet hyperplasia. Leptin treatment normalized blood glucose levels in both groups. Glucose levels also improved spontaneously by 1 year of age, despite sustained lipodystrophy and insulin resistance. Thus, loss of IR is sufficient to disrupt white fat formation, but not brown fat formation and/or maintenance, although it is required for normal BAT function and temperature homeostasis. IGF1R has only a modest contribution to both WAT and BAT formation and function.
Apparatus and methods for probing a material as a function of depth using depth-dependent second harmonic generation (2023)

A method for non-invasively probing at least one physics property of a solid material. In one embodiment, the method has the steps of splitting a photon beam into a first photon beam and a second photon beam, exposing the solid material to the first photon beam to generate a coherent acoustic phonon wave in the solid material at time t, and exposing the solid material to the second photon beam at a time t+Δt, where t+Δt≥t, to generate corresponding second harmonic generation signals, where from the corresponding second harmonic generation signals, the at least one physics property of the solid material is determinable.
A phase I and pharmacokinetic study of intraperitoneal topotecan (2001)

A substantial dose of topotecan can be delivered by the IP route, achieving cytotoxic plasma levels of topotecan, with acceptable toxicity. The recommended dose for further phase II trials is 20 mg/m(2) IP, which enables combination with active doses of other cytotoxic drugs, in view of its limited myelotoxicity when given by this route.
Kinetics of bacterial manganese binding and oxidation in the chemostat (1984)

Abstract The kinetics of manganese binding and oxidation by a marine Pseudomonas sp. have been determined under nutrient‐limited, steady‐state conditions. The first‐order rate constants for manganese binding by steady‐state cultures increased logarithmically with growth rate and were well within the range of values published for a variety of sedimentary and aquatic environments. In addition, the rate of manganese binding per cell appeared to be far faster than the growth kinetics.
Decentralized multi-cell beamforming with QoS guarantees via large system analysis (2015)

This work focuses on the power minimization problem while ensuring target rates in the downlink of a multi-cell multi-user MIMO system wherein L base stations (BSs) of N antennas serve in total K single-antenna user equipments. We assume that the transmit antennas at each BS are correlated and propose a decentralized solution to compute an approximation of the optimal beamforming vectors. The analysis is conducted in the asymptotic regime in which N and K grow large with a given ratio K/N. In particular, the proposed solution relies on the exchange of intercell interference terms whose large system approximations are computed at each BS using knowledge of local transmit correlation matrices and non-local pathlosses. Numerical results are used to evaluate the performance loss of the proposed solution compared to the optimal one and to investigate its accuracy in systems of finite size.
Detection of circulating sarcoma tumor cells using a microfluidic chip-type cell sorter (2019)

Analyses of circulating tumor cells have been shown to be effective for the detection of cancer relapse and prognosis prediction. However, research regarding its utility in sarcoma remains scarce. In this study, the microfluidic chip-type cell sorter On-chip Sort was used to construct a system for detecting circulating sarcoma cells (CSCs). A pilot study using normal fibroblast or sarcoma cell lines was designed to establish a reliable protocol to separate CSCs by On-chip Sort. A single CSC was separated and recovered from 10 ml of whole blood from a patient with locally advanced myxofibrosarcoma. The nonsynonymous mutation for KMT2B p.Ile2602Val identified in the formalin-fixed paraffin-embedded tumor sample was also confirmed in the CSC. Use of the developed protocol may allow CSCs to become an early predictor for metastasis and recurrence of sarcoma. Further, it may aid in optimizing post-operative therapies for patients without metastasis.
Identification and Characterization of Multiple Similar Ligand-binding Repeats in Filamin (2009)

The actin-binding protein filamin links membrane receptors to the underlying cytoskeleton. The cytoplasmic domains of these membrane receptors have been shown to bind to various filamin immunoglobulin repeats. Notably, among 24 human filamin repeats, repeat 17 was reported to specifically bind to platelet receptor glycoprotein Ibalpha and repeat 21 to integrins. However, a complete sequence alignment of all 24 human filamin repeats reveals that repeats 17 and 21 actually belong to a distinct filamin repeat subgroup (containing repeats 4, 9, 12, 17, 19, 21, and 23) that shares a conserved ligand-binding site. Using isothermal calorimetry and NMR analyses, we show that all repeats in this subgroup can actually bind glycoprotein Ibalpha, integrins, and a cytoskeleton regulator migfilin in similar manners. These data provide a new view on the ligand specificity of the filamin repeats. They also suggest a multiple ligand binding mechanism where similar repeats within a filamin monomer may promote receptor clustering or receptor cross-talking for regulation of the cytoskeleton organization and diverse filamin-mediated cellular activities.
Association of Vitamin C, Thiamine, and Hydrocortisone Infusion With Long-term Cognitive, Psychological, and Functional Outcomes in Sepsis Survivors (2023)

ClinicalTrials.gov Identifier: NCT03509350.
Expression of MIG/CXCL9 in Cystic Fibrosis and Modulation of Its Activities by Elastase of &lt;b&gt;&lt;i&gt;Pseudomonas aeruginosa&lt;/i&gt;&lt;/b&gt; (2014)

In cystic fibrosis (CF), colonization of the airways with Pseudomonas aeruginosa is associated with disease deterioration. The mechanism behind the disease progression is not fully understood. The present work shows that the antibacterial chemokine MIG/CXCL9 is present in the airways and in sputum of CF patients. MIG/CXCL9 showed high bactericidal activity against. P. aeruginosa, including some strains from the airways of CF patients. Full-length MIG/CXCL9 was detected in sputum from healthy controls and CF patients colonized with P. aeruginosa. However, degraded MIG/CXCL9 was only found in CF sputum. In vitro, elastase of P. aeruginosa cleaved off a fragment of similar size and two additional fragments from MIG/CXCL9. The fragments showed less bactericidal activity against P. aeruginosa compared with the full-length protein. The fragments did not activate the MIG/CXCL9 receptor CXCR3 (expressed e.g. by NK cells, mast cells, and activated T cells) but instead displayed noncompetitive inhibition. In vitro, a decrease in CXCR3-bearing cells was found within and in the proximity of the bronchial epithelium of CF lung tissue compared with controls. Taken together, both bactericidal and cell-recruiting activities of MIG/CXCL9 are corrupted by P. aeruginosa through release of elastase, and this may contribute to impaired airway host defense in CF.
MASSA Savina Dolores, Ma fille folie (2015)

National audience
Influence of pyrolysis and Silicon infiltration on the properties of CMC parts shaped by composite flow molding (2021)

Abstract This paper investigates the influence of thermal process parameters on the mechanical properties and final microstructure of SiC‐based ceramic matrix composites parts shaped by composite flow molding, and converted into ceramics by pyrolysis and reactive silicon melt infiltration. Green composite parts consisted of a thermoplastic matrix (PEEK) reinforced with continuous carbon fibers. Parts were pyrolyzed between 960°C and 2000°C and subsequently Si infiltrated between 1600°C and 1800°C, with a dwell time of 1 to 3 h. An experimental plan was defined following a design of experiment (DOE) approach. At high pyrolysis and infiltration temperatures, samples show higher modulus of rupture (280 MPa) and flexural modulus (158 GPa). A qualitative image analysis was adopted to estimate the CMC composition. A predictive model allowing the estimation of the composite toughness as a function of the significant process parameters was derived by applying the DOE methodology and the analysis of variances. The model is based on the observed correlations between the process parameters and their influence on the material property of interest. The most influential parameters are the silicon infiltration temperature and the dwell time. This is due to their control over the reaction kinetics between C and Si to obtain SiC.