gebhart/patrepeval huggingface dataset:
Defining the epigenetic status of blood cells using a cyanine-based fluorescent probe for PRMT1 (2018)

Dynamic regulation of histone modification enzymes such as PRMT1 (protein arginine methyltransferase 1) determines the ordered epigenetic transitions in hematopoiesis. Sorting cells according to the expression levels of histone modification enzymes may further define subpopulations in hematopoietic lineages with unique differentiation potentials that are presently defined by surface markers. We discovered a vital near infrared dye, E84, that fluoresces brightly following binding to PRMT1 and excitation with a red laser. The staining intensity as measured by flow cytometry is correlated with the PRMT1 expression level. Importantly, E84 staining has no apparent negative effect on the proliferation of the labeled cells. Given that long-term hematopoietic stem cells (LT-HSCs) produce low levels of PRMT1, we used E84 to sort LT-HSCs from mouse bone marrow. We found that SLAM (the signalling lymphocyte activation molecule family) marker-positive LT-HSCs were enriched in the E84<sup>low</sup> cell fraction. We then performed bone marrow transplantations with E84<sup>high</sup> or E84<sup>low</sup> Lin<sup>-</sup>Sca1<sup>+</sup>Kit<sup>+</sup> (LSK) cells and showed that whole blood cell lineages were successfully reconstituted 16 weeks after transplanting 200 E84<sup>low</sup> LSK cells. Thus, E84 is a useful new tool to probe the role of PRMT1 in hematopoiesis and leukemogenesis. Developing E84 and other small molecules to label histone modification enzymes provides a convenient approach without modifying gene loci to study the interaction between hematopoietic stem/progenitor cell epigenetic status and differentiation state.
Effects of divalent cations on amino acid and divalent cation fluxes in gills of the bivalve mollusc, <i>Mytilus californianus</i> (1980)

Effects of the deletion of Ca++, Mg++, or Ca++ and Mg++ on the leakage of primary amines, Ca++, and Mg++ from gills of Mytilus californianus were studied. In the absence of Ca++ the gills leak primary amines and Ca++ for about 30 minutes, after which the primary amines are reabsorbed. In the absence of Mg++ the gills leak primary amines, with no net reabsorption for at least 90 minutes, and show rapid leakage of Mg++. In the absence of both Ca++ and Mg++ amino acid leakage is initially less than in the absence of only one of the cations. Measurements of the kinetics of leakages of Ca++ and Mg++ are consistent with the hypothesis that both ions are associated with the cell surface, but the affinity of Ca++ for the cell surface is greater than that of Mg++. In the absence of Mg++, the influx of 14C-glycine was depressed to about 1/3 that seen in controls, while deletion of Ca++ had no effect on the rate of influx. Increasing the [Mg++] stepwise between 5 x 10(-6) and 1 x 10(-1) M results in a sigmoidal increase in the rate of influx of 14C-glycine and a hyperbolic increase in the y-intercept of influx curves.
A 60GHz 144-element phased-array transceiver with 51dBm maximum EIRP and ±60° beam steering for backhaul application (2018)

The 802.11ad standard (WiGig) provides throughput speeds of multi-Gb/s covering tens of meters and uses beamforming in the four 2GHz-wide channels in the 60gHz ISM band. Conventional backhaul solutions, on the other hand, are designed with high gain directional antennas with no electronic beam steering and have high cost for installation and alignment of antennas. This paper presents a full-featured 802.11ad chipset with 144-element phased array using a tiled approach and CMOS IPs developed for WiGig to address low-cost municipal WiFi, small-cell backhauling, and broadband to home covering the last mile. Designed with a link budget of 120dB+, the phased-array solution can be mounted on top of lamp posts and roof-top buildings covering a 200m LOS, and with tens of hops a range of 2km can be covered from a single point of fiber. Moreover, the steerable phased arrays enable dynamic routing optimization between hopping nodes in a mesh network.
Distribution of Attention, Situation Awareness and Workload in a Passive Air Traffic Control Task: Implications for Operational Errors and Automation (1998)

A study was conducted to investigate factors underlying operational errors (OEs) in en route air traffic control (ATC). Twenty active-duty controllers watched recreations of OEs and were asked to report on their situation awareness and workload. A total of 14 OEs were examined. Responses were analyzed to determine how the subjects allocated their attention in viewing the scenarios. While observed patterns probably reflect necessary prioritization schemes, attention strategies identified in this study can be linked to data on factors underlying OEs. Both objective taskload, as indicated by the number of aircraft being controlled, and subjective workload were found to be related to the controllers’ ability to report situation awareness information. Workload was found to be higher at the time of the OE than at the other stop during the recreation. During high workload, controllers appeared to pay less attention to certain aircraft and variables in order to maintain awareness of more important information. This research has implications for potential problems in situation awareness under passive monitoring conditions that may be present if certain forms of automation are introduced in the future ATC system.
Intravenous Grafts Recapitulate the Neurorestoration Afforded by Intracerebrally Delivered Multipotent Adult Progenitor Cells in Neonatal Hypoxic-Ischemic Rats (2008)

Once hypoxic-ischemic (HI) injury ensues in the human neonate at birth, the resulting brain damage lasts throughout the individual's lifetime, as no ameliorative treatments are currently available. We have recently shown that intracerebral transplantation of multipotent adult progenitor cells (MAPCs) results in behavioral improvement and reduction in ischemic cell loss in neonatal rat HI-injury model. In an attempt to advance this cellular therapy to the clinic, we explored the more practical and less invasive intravenous administration of MAPCs. Seven-day-old Sprague-Dawley rats were initially subjected to unilateral HI injury, then 7 days later received intracerebral or intravenous injections of allogeneic rat MAPCs. On post-transplantation days 7 and 14, the animals that received MAPCs via the intracerebral or intravenous route exhibited improved motor and neurologic scores compared with those that received vehicle infusion alone. Immunohistochemical evaluations at day 14 after transplantation revealed that both intracerebrally and intravenously transplanted MAPCs were detected in the ischemic hippocampal area. The degree of hippocampal cell preservation was almost the same in the two treatment groups and greater than that in the vehicle group. These results show that intravenous delivery of MAPCs is a feasible and efficacious cell therapy with potential for clinical use.
Understanding Palladium Acetate from a User Perspective (2016)

Abstract Invited for the cover of this issue is the group of Thomas J. Colacot at Johnson Matthey. The image depicts palladium acetate as a card trick that still needs to be practiced. Read the full text of the article at 10.1002/chem.201601450 .
COVID-19 biorepository-Qatar (2021)

Background: The unpresented global spread of the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and its resulting disease (COVID-19) is characterized as one of the greatest global public health crises of the recent decades. The COVID-19 Biorepository is Qatar's National project aiming to support the extraordinary demand on high-quality, curated clinical data and biological samples contributing to research related to the understanding of, and response to, the COVID-19 pandemic and its impacts in Qatar and worldwide. Methods: COVID-19 diagnosed patients, who were Qatar residents and they could communicate in Arabic, English, Hindi and Urdu, were eligible to participate in the study. Participants were recruited from the main three COVID-19 public hospitals and isolation facilities in Doha, Qatar for a 7-month period (March-September 2020). Consented participants were followed up on a weekly basis until recovery, and then monthly for a year. Sociodemographic and clinical data were collected in electronic questionnaires via a face-to-face interview by trained QBB staff together with biological samples. COVID-19 Rapid Antibodies tests together with Anti- SARS-COV-2- seroprevalence tests are in place to investigate the development and durability of antibodies against SARS-CoV-2 infection. Results: A total of 2097 consented participants were recruited up to September 2020. The population is multinational (n = 45 different nationalities)withmales (N = 1050;50.07%) and females (N = 1047;49.93%) and a mean age of 41 years (SD, 15.5). 61.0% of the participants had at least one follow up while 27%adhered to monthly follow up visits. Data have been collected for 99.7% of the participants, while the follow up process is ongoing. In total there are 107,171 high quality specimens in the biorepository including plasma, erythrocytes, buffy coat, serum, PAXgene Whole Blood, Nasopharyngeal secretions and DNA. 61.6% of the participants were asymptomatic or with mild symptoms, 12.4% developed moderate symptoms, 15.1% developed severe symptoms and 11.0% were reported as critical. The 5.7% (n = 121) of the participants were deceased after hospitalization. Conclusion: COVID-19 biorepository is a National asset for Qatar. It is expected to provide high quality data and biological samples to the scientific community that can be used to illuminate the pathophysiology of and to identify markers of disease prognosis of COVID-19 in Qatar and worldwide.
Analysis of Age-Related Modifications of Lower Limb Motor Control Strategies by Using a Wearable Biomechatronic System (2005)

The main aim of this work was to identify an experimental method for the quantitative assessment of the motor performance of elderly people, during motor tasks requiring also different cognitive efforts. Specifically, a dual task approach was used to investigate age-related deficits in the ability to walk or climb stairs, and to concurrently perform various categories of cognitive tasks. A wearable biomechatronic system was used to measure the kinematics of the lower limb, during the task execution. Parameters derived from gait analysis were used for the quantitative assessment of examined subjects motor performances. Preliminary results showed that the proposed method can provide accurate and reliable measurements, which could be useful to deep inside the comprehension of the age-related deficits of lower limb motor control strategies.
ChemInform Abstract: Reactivity of Carbohydrate Radicals Derived from Iodo Sugars and Dibenzoyl Peroxide. Homolytic Heteroaromatic and Aromatic Substitution, Reduction, and Oxidation. (1994)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
The Discrete Memoryless Arbitrarily Varying Wiretap Channel (2017)

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Mobichart for modeling mobile computing tasks (2004)

We discuss the specialty and the limitations found in the mobile computing environment to motivate the reader on the necessity of having methods for developing mobile computing applications in the object oriented paradigm. It is shown that existing specification models, like Objectchart, do not model effects due to location awareness and other typical phenomena, like hands-off and hands-on. We previously introduced Mobichart, a notation to specify mobile computing applications (Acharya, S. et al., Proc. 36th Hawaii Int. Conf. on System Sciences, 2003). We show that Mobichart can address the drawbacks of Objectchart and can be used to model mobile computing applications.
Ground-Semiconductor-Ground Guiding Phenomena of Noise Coupling in CMOS Substrate (2007)

Ground-semiconductor-ground (GSG) transmission line (TL) is proposed and investigated thoroughly for the assessment of CMOS signal integrity problem at multi-GHz frequency regime. The GSG TL models the practical implementation of CMOS logic layout, which consists of power line, Nwell line (semiconductor), Pwell line (semiconductor), and ground line. Rigorous field-theory analyses and experiments show that GSG TL supports the CPW (coplanar waveguide) mode for the particular case study, which also demonstrate the slow-wave factor can be as high as 50 at 1 GHz and decrease to 10 at 10 GHz, pointing to the fact that the slow-wave phenomena will be more pronounced for CMOS electronics operated beyond tens of GHz
Quantitative analysis of polymorphic mixtures of carbamazepine by Raman spectroscopy and principal components analysis (2004)

Abstract The polymorphic behaviour of drugs is a major concern of the pharmaceutical industry as it may have considerable formulation, therapeutic, legal and commercial implications. It is therefore crucial to be able adequately to identify and quantify different polymorphic forms of drugs as early as possible in the drug discovery and development process. The aim of the present study was to investigate the application of Raman spectroscopy and principal components analysis (PCA) to the quantitative analysis of polymorphic mixtures of carbamazepine forms III and I, based on both individual peak areas and heights in the Raman spectra and whole wavenumber ranges. Various linear regressions indicated that in general using every fourth wavenumber value of a larger region of the Raman spectrum gives better quantitative results than choosing defined peak areas and peak heights, as the spectral differences between the polymorphs were only subtle. Baseline correction of the spectral data did not appear to improve the quantitative analysis, with detection and quantification limits being the lowest (0.33 and 1.09%, respectively) and R 2 being the highest (0.96) when using every fourth wavenumber value from 2950 to 3100 cm −1 as input data for the PCA analysis without baseline correction. This study demonstrated that PCA of Raman spectroscopic data provides a sensitive method for the quantitative analysis of polymorphic forms, with a quantitation limit of &lt;2%, if the spectral input data are selected carefully. In the absence of clear and distinct spectral differences at certain wavenumbers between the polymorphic forms, selection of larger spectral regions that contain most of the detectable differences between the two forms appears to be the best data selection technique. Copyright © 2004 John Wiley &amp; Sons, Ltd.
Efectos de dos métodos de entrenamiento de fuerza sobre el Índice de Bosco en jugadoras de balonmano de División de Honor (Effects of two strength training methods in Bosco Index on elite handball female player) (2007)

El presente trabajo es un estudio de ámbito deportivo, donde se pretende conocer y mejorar la manifestación de fuerza menos desarrollada, modificando el Índice de Bosco, utilizando dos programas de entrenamiento de la fuerza por contraste acentuado en la serie. Para ello, se ha seleccionado una muestra de nueve jugadoras de división de honor con edades comprendidas entre los 18 y 32 años siendo divididas en dos grupos. Un grupo formado por aquellas jugadoras que han obtenido en el pretest unos valores más bajos en el Índice de Bosco, que llevará a cabo un programa de entrenamiento de fuerza por contraste acentuado en la serie con cargas máximas y pliometría y el otro grupo por las que consiguieron unos valores más altos, que realizarán un programa de entrenamiento de fuerza por contraste acentuado en la serie con cargas medias y pliometría, a los cuales serán sometidas durante un periodo de diez semanas, ejecutando dos sesiones en cada una. Los resultados obtenidos de la investigación muestran diferencias significativas en el pretest entre ambos grupos, lográndose igualar los Índices de Bosco de ambos grupos en el test intermedio y postest.Abstract: The current work is a sports field study, where the purpose is to know and to improve the less develop strength display, altering the Bosco Index, using two strength training programs by series stressed contrast. For that, it has been choosing a sample of nine elite female players between 18 and 32 years old, being splat in two groups up. One group was set for those players whose got the lowest Bosco Index scores, and made a strength training program with maximum loads and plyometric. The other group was set with the players whose got the highest Bosco Index scores, and did the strength training program with medium loads and plyometric. Both groups were training during ten weeks, twice a week. The research outcomes show significant differences in both groups during pretest, achieving to make equal the Index Bosco during the intermediate test and post test.
&lt;title&gt;Rapid multiexcitation spectrofluorimeter for in-vivo tissue diagnosis&lt;/title&gt; (1997)

We have designed, fabricated and tested a compact excitation-emission spectrofluorimeter with optical fiber light delivery and collection for use in rapid analysis of tissues in a clinical setting. This system provides ten different excitation wavelengths, permitting collection of all ten emission spectra from biological tissues in approximately 500 ms. It uses a nitrogen laser which pumps a sequence of dyes placed in cuvettes on a rotating wheel. All dye cuvettes utilize a common optical resonant cavity, which simplifies the coupling of the different excitation wavelengths into the delivery fiber of an optical fiber probe. A filter wheel containing one long-pass filter for each excitation wavelength is synchronized with the dye wheel for compactness and ease of control. A white light excitation source permits acquisition of the tissue diffuse reflectance spectrum on each cycle. The fiber optic probe is composed of seven optical fibers arranged at the distal tip in the form of a central delivery fiber surrounded by six collection fibers, with an optical shield. Return fluorescence and reflected light are dispersed by a small spectrograph and detected by a photodiode array detector. The system can collect a single shot spectrum from biological tissue with a signal-to-noise ratio in excess of 50:1. This unique design for providing rapidly variable excitation wavelengths facilitates the collection of multi- excitation fluorescence spectra for the diagnosis of diseases in vivo.
Investigation of biomass concentration, lipid production, and cellulose content in <i>Chlorella vulgaris</i> cultures using response surface methodology (2013)

The microalgae Chlorella vulgaris produce lipids that after extraction from cells can be converted into biodiesel. However, these lipids cannot be efficiently extracted from cells due to the presence of the microalgae cell wall, which acts as a barrier for lipid removal when traditional extraction methods are employed. Therefore, a microalgae system with high lipid productivity and thinner cell walls could be more suitable for lipid production from microalgae. This study addresses the effect of culture conditions, specifically carbon dioxide and sodium nitrate concentrations, on biomass concentration and the ratio of lipid productivity/cellulose content. Optimization of culture conditions was done by response surface methodology. The empirical model for biomass concentration (R(2) = 96.0%) led to a predicted maximum of 1123.2 mg dw L(-1) when carbon dioxide and sodium nitrate concentrations were 2.33% (v/v) and 5.77 mM, respectively. For lipid productivity/cellulose content ratio (R(2) = 95.2%) the maximum predicted value was 0.46 (mg lipid L(-1) day(-1) )(mg cellulose mg biomass(-1) )(-1) when carbon dioxide concentration was 4.02% (v/v) and sodium nitrate concentration was 3.21 mM. A common optimum point for both variables (biomass concentration and lipid productivity/cellulose content ratio) was also found, predicting a biomass concentration of 1119.7 mg dw L(-1) and lipid productivity/cellulose content ratio of 0.44 (mg lipid L(-1) day(-1) )(mg cellulose mg biomass(-1) )(-1) for culture conditions of 3.77% (v/v) carbon dioxide and 4.01 mM sodium nitrate. The models were experimentally validated and results supported their accuracy. This study shows that it is possible to improve lipid productivity/cellulose content by manipulation of culture conditions, which may be applicable to any scale of bioreactors.
Government intervention and the problem of knowledge in education policy (2005)

This paper seeks to explore possible connections between the range of work in the sociology of education concerned with the role of government in promoting marketisation and choice in education and the issues around knowledge and the curriculum that I and others such as Muller (2000) in Capetown and Moore (Moore 2004) in Cambridge have been concerned with.
Virtual Special Issue: Epigenetics 2022 (2022)

ADVERTISEMENT RETURN TO ISSUEEditorialNEXTVirtual Special Issue: Epigenetics 2022Courtney C. AldrichCourtney C. AldrichMore by Courtney C. Aldrichhttps://orcid.org/0000-0001-9261-594X, Félix CalderónFélix CalderónMore by Félix Calderónhttps://orcid.org/0000-0003-0486-6883, Stuart J. ConwayStuart J. ConwayMore by Stuart J. Conwayhttps://orcid.org/0000-0002-5148-117X, Chuan HeChuan HeMore by Chuan Hehttps://orcid.org/0000-0003-4319-7424, Jacob M. HookerJacob M. HookerMore by Jacob M. Hookerhttps://orcid.org/0000-0002-9394-7708, Donna M. HurynDonna M. HurynMore by Donna M. Hurynhttps://orcid.org/0000-0001-5542-4968, Craig W. LindsleyCraig W. LindsleyMore by Craig W. Lindsleyhttps://orcid.org/0000-0003-0168-1445, Dennis C. LiottaDennis C. LiottaMore by Dennis C. Liottahttps://orcid.org/0000-0002-7736-7113, and Christa E. MüllerChrista E. MüllerMore by Christa E. Müllerhttps://orcid.org/0000-0002-0013-6624Cite this: ACS Pharmacol. Transl. Sci. 2022, 5, 10, 829–834Publication Date (Web):September 9, 2022Publication History Received22 August 2022Published online9 September 2022Published inissue 14 October 2022https://doi.org/10.1021/acsptsci.2c00169Copyright © Published 2022 by American Chemical SocietyRIGHTS & PERMISSIONSArticle Views533Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (1 MB) Get e-AlertsSUBJECTS:Genetics,Infectious diseases,Inhibitors,Medicinal chemistry,Peptides and proteins Get e-Alerts