gebhart/patrepeval huggingface dataset:
EVI1 carboxy-terminal phosphorylation is ATM-mediated and sustains transcriptional modulation and self-renewal via enhanced CtBP1 association (2018)

The transcriptional regulator EVI1 has an essential role in early hematopoiesis and development. However, aberrantly high expression of EVI1 has potent oncogenic properties and confers poor prognosis and chemo-resistance in leukemia and solid tumors. To investigate to what extent EVI1 function might be regulated by post-translational modifications we carried out mass spectrometry- and antibody-based analyses and uncovered an ATM-mediated double phosphorylation of EVI1 at the carboxy-terminal S858/S860 SQS motif. In the presence of genotoxic stress EVI1-WT (SQS), but not site mutated EVI1-AQA was able to maintain transcriptional patterns and transformation potency, while under standard conditions carboxy-terminal mutation had no effect. Maintenance of hematopoietic progenitor cell clonogenic potential was profoundly impaired with EVI1-AQA compared with EVI1-WT, in particular in the presence of genotoxic stress. Exploring mechanistic events underlying these observations, we showed that after genotoxic stress EVI1-WT, but not EVI1-AQA increased its level of association with its functionally essential interaction partner CtBP1, implying a role for ATM in regulating EVI1 protein interactions via phosphorylation. This aspect of EVI1 regulation is therapeutically relevant, as chemotherapy-induced genotoxicity might detrimentally sustain EVI1 function via stress response mediated phosphorylation, and ATM-inhibition might be of specific targeted benefit in EVI1-overexpressing malignancies.
Improvement of renal function prior to liver transplantation is not associated with better long-term renal outcome or survival (2021)

Improvement of renal function immediately prior to LT, either spontaneously or following terlipressin therapy, did neither ameliorate long-term renal outcome nor survival in LT recipients. Future studies need to clarify the impact of terlipressin in HRS on the transplant waiting time in LT candidates.
Quality of 186 child brain spectra using motion and B0 shim navigated single voxel spectroscopy (2013)

Prospective motion and B0 correction provides high quality repeatable spectra. The study found that most children moved between acquisitions and a substantial number moved during acquisitions.
Impaired adhesion of neutrophils expressing Slc44a2/HNA-3b to VWF protects against NETosis under venous shear rates (2021)

Genome-wide association studies linked expression of the human neutrophil antigen 3b (HNA-3b) epitope on the Slc44a2 protein with a 30% decreased risk of venous thrombosis (VT) in humans. Slc44a2 is a ubiquitous transmembrane protein identified as a receptor for von Willebrand factor (VWF). To explain the link between Slc44a2 and VT, we wanted to determine how Slc44a2 expressing either HNA-3a or HNA-3b on neutrophils could modulate their adhesion and activation on VWF under flow. Transfected HEK293T cells or neutrophils homozygous for the HNA-3a- or HNA-3b-coding allele were purified from healthy donors and perfused in flow chambers coated with VWF at venous shear rates (100 s-1). HNA-3a expression was required for Slc44a2-mediated neutrophil adhesion to VWF at 100 s-1. This adhesion could occur independently of β2 integrin and was enhanced when neutrophils were preactivated with lipopolysaccharide. Moreover, specific shear conditions with high neutrophil concentration could act as a "second hit," inducing the formation of neutrophil extracellular traps. Neutrophil mobilization was also measured by intravital microscopy in venules from SLC44A2-knockout and wild-type mice after histamine-induced endothelial degranulation. Mice lacking Slc44a2 showed a massive reduction in neutrophil recruitment in inflamed mesenteric venules. Our results show that Slc44a2/HNA-3a is important for the adhesion and activation of neutrophils in veins under inflammation and when submitted to specific shears. The fact that neutrophils expressing Slc44a2/HNA-3b have a different response on VWF in the conditions tested could thus explain the association between HNA-3b and a reduced risk for VT in humans.
Output pulse waveform correction by dual modulation of three-terminal Fabry-Perot diode laser (2002)

We report on the possibility of improvement of output pulse waveform by dual modulation of three-terminal MQW FP laser. High quality eye pattern and low intensity noise operation may be obtained by using dual modulation.
Self Discharge of Magnesium-Sulfur Batteries Leads to Active Material Loss and Poor Shelf Life (2020)

&lt;p&gt;Due to its high theoretical energy density and relative abundancy of active materials, the magnesium-sulfur battery has attracted research attention in recent years. A closely related system, the lithium-sulfur battery, can suffer from serious self-discharge behavior. Until now, the self-discharge of Mg-S has been rarely addressed, and even then only indirectly. Herein, we demonstrate for a wide variety of Mg-S electrolytes and conditions that Mg-S batteries also suffer from serious self-discharge. For a common Mg-S electrolyte, we identify a multi-step self-discharge pathway. Covalent S&lt;sub&gt;8&lt;/sub&gt; diffuses to the metal Mg anode and is converted to ionic Mg polysulfide in a non-faradaic reaction. Mg polysulfides in solution are found to be meta-stable, continuing to react and precipitate as solid Mg&lt;sub&gt;y&lt;/sub&gt;S&lt;sub&gt;x&lt;/sub&gt; species during both storage and active use. Mg-S electrolytes from the early, middle, and state-of-the-art stages of the Mg-S literature are all found to enable the self-discharge. The self discharge behavior is found to decrease first cycle discharge capacity by at least 32 %, and in some cases up to 96 %, indicating this is a phenomenon of the Mg-S chemistry that deserves focused attention.&lt;/p&gt;
The characterisation and regulation of type 1 immune responses in psoriatic arthritis (2002)

Vascular changes are consistent early findings in patients with SSc and often precede the development of fibrosis.Despite a significant reduction in the capillary density, there is paradoxically no sufficient angiogenesis in the skin of SSc patients.By using a pO 2 histograph, we showed that low pO 2 values are overt in involved skin of patients with SSc.In vitro, real-time PCR revealed a 3.7-fold upregulation of the potent angiogenic growth factor VEGF in SSc fibroblasts after hypoxic exposure compared to normoxic controls.In situ hybridization for VEGF in skin biopsies of patients with SSc showed an overexpression of VEGF mRNA by fibroblasts and mononuclear infiltrates, whereas its expression was limited to keratinocytes in healthy control biopsies.In contrast to the SSc skin, HIF-1 alpha protein was found to be coexpressed with VEGF in healthy skin samples, indicating that the constitutive VEGF synthesis in the skin is driven by this transcription factor.Additionally, we showed that the lack of angiogenesis in SSc is not due to a reduced bioavailability of the overexpressed VEGF, since the VEGF receptors Flk-1 and Flt-1 were found to be expressed on endothelial cells of patients with SSc, but not in healthy controls, and since SSc patients had severely elevated serum levels of VEGF compared to healthy controls.Despite the enhanced levels of VEGF, serum samples of SSc patients did not induce angiogenesis in the vivo chorion allantois membrane assay, indicating that the proangiogenic effects of VEGF may be outweighed by angiostatic factors.The hypothesis that VEGF synthesis has to be above an individual threshold in SSc patients to induce angiogenesis was further strengthened by the finding that patients without fingertip ulcers had significantly higher levels than patients with fingertip ulcers.Interestingly, the angiostatic factor endostatin was elevated in a subset of patients and thus may counteract directly the bio-logic effects of VEGF in SSc patients.Serum levels of VEGF were also correlated significantly with disease severity parameters including antitopoisomerase antibodies.These results suggest that therapeutic application of VEGF by either gene transfer or as a recombinant protein might be a novel option in SSc.
Research Guides: Education: Civic Engagement - Social Studies CBAs: Social Studies Areas (2010)

EWU in cooperation with West Valley School District is preparing materials for K-12 civic engagement.
The Path to an RSV Vaccine (2015)

A thorough and up-to-date review of vaccinology research in age of omics technologies. Part I covers the key innovations that are dramatically changing the field. In Part II chapters are focused on vaccines for diseases for which current treatment is ineffective. Essential reading for everyone working in vaccine R and D.
Cobalamin‐Dependent Methionine Synthase (2004)

Abstract The methylcobalamin‐dependent methionine synthase catalyzes the transfer of a methyl group from 5‐methyltetrahydrofolate to homocysteine. This constitutes the terminal step in the de novo synthesis of methionine in eubacteria. In mammals, the enzyme is part of a salvage pathway involved in the conservation of limiting amounts of this essential amino acid. The Escherichia coli enzyme is a modular protein consisting of four distinct regions each with a specific function in the overall catalytic cycle. Crystal structures of two of these modules (the B 12 ‐binding fragment and the C‐terminal activation domain) have been determined.
Transient Performance Analysis of a Multicorrelator Signal Quality Monitor (2001)

The ability to monitor and detect problematic distortions
in the received GPS-SPS signal is a task of critical
importance. Detection of these satellite signal anomalies
or “evil waveforms” (EWFs) can be accomp lished using
detailed monitoring of the correlation peak. Using the
proposed “2nd-Order Step” (2OS) Threat Model for evil
waveforms, previous analysis has shown that, in steady
state, monitoring sufficient to satisfy GBAS and SBAS
requirements for Category I precision approaches may be
obtained from a receiver design that requires minimal
modifications to existing GPS hardware. In other words,
ignoring time-to-alarm requirements, it has been shown
that these anomalous waveforms can be detected using a
practical, multicorrelator SQM implementation—defined
as SQM2b. The ability of the monitor receiver to detect
hazardous evil waveforms within the time to alarm,
however, is necessary to guarantee airborne users never
experience hazardously misleading information.
This paper shows that, without modification, SQM2b
(previously validated by steady-state analysis alone) does
not promptly mitigate this integrity threat. Further, it
suggests that modifying the smoothing filter for the
detection metrics can slightly decrease the response time
required by the monitor detect these waveforms. More
significantly, leveraging the same measurements taken
from SQM2b, it introduces an additional nonlinear
detection test that improves the sensitivity of the monitor to
detectable anomalous waveforms. The paper presents
analytical results which verify that these modifications can
detect the hazardous waveforms within the 6-second timeto-
alarm required by LAAS. Finally, it validates the
performance of this test using experimental data taken from
a real-time evil waveform-generating hardware.
Isolation and partial sequencing of the human prothymosin α gene family (1989)

Prothymosin alpha and thymosin alpha 1 are believed to be thymus-derived, hormone-like materials with immunomodulatory functions performed outside the cell. These functions are inconsistent with the existence of a full length cDNA clone that does not encode an amino-terminal signal peptide or several consecutive hydrophobic residues. A study of the prothymosin alpha mRNAs and genes was undertaken in search of evidence for secreted forms of the protein. Prothymosin alpha mRNA was localized exclusively on free, rather than membrane-bound, polysomes. Upon screening cosmid and plasmid libraries totaling 2 X 10(6) clones, a gene family consisting of six members was identified. Sequence information from the 5'-ends of all the genes indicated that none encodes an amino-terminal signal peptide. One of the genes, apparently by means of alternate splicing, gives rise to two prothymosin alpha mRNAs, one of which has an additional internal glutamic acid codon with respect to the other. Comparison of the translated nucleic acid sequences of the five remaining genes with those encoded in the mRNAs revealed 30-98% homology in the first 50 amino acids. These five genes appear to be processed genes and/or pseudogenes. The localization of prothymosin alpha mRNAs on free polysomes, together with the partial nucleotide sequences of the genes, strongly suggest an intracellular function for prothymosin alpha. Therefore, the possibility must be raised that prothymosin alpha and its peptide derivatives act as xenobiotics when introduced into assays of immune function.
Mechanical vs Non-Mechanical Mesh Fixation in Open Retromuscular Ventral Hernia Repair: A Comparative Analysis from the Americas Hernia Society Quality Collaborative (2018)

Etemad, Shervin A.; Huang, LiChing PhD; Phillips, Sharon E. MSPH; Stewart, Thomas G. PhD; Pierce, Richard A. MD, PhD, FACS; Schneeberger, Steven; Poulose, Benjamin K. MD, MPH, FACS Author Information
CAR T-cells: for who and how? (2020)

L’utilisation des cellules T à récepteur antigénique chimérique (CAR-T) anti-CD19 dans les hémopathies B – en particulier dans les leucémies aiguës lymphoblastiques B (LAL-B) et les lymphomes diffus à grandes cellules B (LBDGC) en rechute ou réfractaires – est croissante. De ce fait, la connaissance des indications, des contre-indications et des critères d’éligibilité est fondamentale afin d’optimiser l’utilisation de cette thérapeutique innovante. Le parcours de soin des patients et leur adressage dans des centres CAR-T sont désormais effectifs. Une collaboration étroite entre le centre d’origine du patient et le centre référent CAR-T est la clef d’une bonne prise en charge. Entre l’inclusion du patient dans la procédure CAR-T et la réinjection, le patient a souvent besoin d’une chimiothérapie d’attente. Nous détaillerons dans cet article les modalités pratiques d’arrêt des différents agents antinéoplasiques et immunosuppresseurs avant cytaphérèse et avant réinjection. L’information au patient est donnée de façon pluridisciplinaire, par les médecins mais aussi par un infirmier de coordination, sur le bénéfice, les effets indésirables et les modalités pratiques. Récemment, des groupes d’experts ont proposé des algorithmes thérapeutiques pour les deux toxicités les plus fréquentes : le syndrome de relargage cytokinique et la toxicité neurologique centrale, permettant ainsi d’harmoniser les pratiques des différents centres CAR-T. Nous aborderons également d’autres complications : les cytopénies, l’hypogammaglobulinémie et les infections. La pluridisciplinarité est la règle, avec l’implication des hématologues, des réanimateurs, des infectiologues, des neurologues, des neuropsychologues, des biologistes, des médecins nucléaires, des radiologues, des pharmaciens, des médecins du centre d’aphérèse et des coordinateurs. La rédaction de procédures de prise en charge de ces complications et des interactions entre ces différents professionnels fait partie intégrante du dossier d’accréditation des centres CAR-T.
A CMOS image sensor for focal-plane low-power motion vector estimation (2002)

This paper presents a CMOS image sensor which captures intermediate pictures at 480 frames/s and a fully accumulated picture at 30 frames/s. The CMOS image sensor is for integrating a low-power motion vector estimation (MVE) engine using the iterative block matching algorithm proposed by the authors.
The utility of noninvasive mapping in persistent atrial fibrillation ablation (2015)

Noninvasive mapping allows unprecedented panoramic beat-to-beat mapping of atrial fibrillation (AF) and its underlying dynamic processes. This provides an individualized framework to guide catheter ablation of persistent AF. This chapter discusses the utility of noninvasive mapping in persistent AF ablation, including the methodology, the identification of localized AF reentrant and focal drivers, current experience and results, strengths and limitations, and future areas of study. Body surface noninvasive mapping has been validated in numerous cardiac arrhythmias, including focal atrial tachycardias (ATs), macroreentrant ATs, Wolff-Parkinson-White syndrome, ventricular arrhythmias and AF. Two main sets of data are required during noninvasive mapping: electrographic potentials recorded from the body surface and geometries of the atria and torso. Noninvasive mapping also has the potential to serve as a risk-stratifying tool for AF, providing a noninvasive assessment of electrical remodeling and AF processes that complements magnetic resonance imaging (MRI) of atrial fibrosis.
4-Substituted-piperazin-1-yl-carbonyl-pyrazol derivatives: A new class of highly potent cytotoxic compounds with inhibitory effects on tubulin polymerization (2004)

5424 4-Phenyl-1-piperazinyl-carbonyl substituted, nitrogen containing heterocycles have been discovered at Zentaris as a new class of potent, synthetic, small molecule tubulin binders with strong antiproliferative activity. The lead structure of this class, D-82318, proved to be a potent inhibitor of in vivo tumor growth in different xenograft models including mammary and renal cancers. As part of our efforts to expand structural diversity as well as to optimize bioavailability parameters such as solubility and metabolic stability for this compound class, we synthesized a small focused compound library. D-87264, the best representative of this series so far, has been identified by a cell based XTT assay on human tumor cell lines, namely SKOV3 (ovarian carcinoma), SF268 (CNS cancer, glioma), KB/HeLa (cervix carcinoma), and NCI-H460 (non-small cell lung cancer), and has shown good cytotoxic activity against these cell lines with IC50 values ranking from 14nM to 34nM. For further characterization the in vitro antiproliferative activity against a panel of more than 30 established human tumor cell lines including multidrug resistant (MDR1) phenotypes was tested with an average IC50 value of 40nM. Mode-of-action studies revealed that D-87264 effectively inhibits tubulin polymerization (IC50 1270nM). Dividing KB/Hela cells were arrested in the G2/M phase of the cell cycle with an IC50 value of 34nM and subsequently underwent apoptosis. Different apoptotic parameters were detected such as increase of caspase activity, cell membrane alterations or DNA fragmentation. Results of competing experiments with [3H] colchicine and [3H] paclitaxel suggest that the compound interacts with the colchicine binding site of β-tubulin (IC50 760nM for colchicine and no competition with paclitaxel). These evaluations were supported by docking experiments. For testing of in vivo activity, a maximal tolerated dose of 75mg/kg/d for p.o. (Q3dx6) administration was found in nude mice. First experiments with tumor bearing nude mice have been performed, and showed promising antitumoral effects in a renal cancer xenograft model after oral administration.
ChemInform Abstract: Synthesis of Nalbuphine from Oripavine via N‐Demethylation of N‐Cyclobutylmethyl Oripavine. (2012)

Abstract The synthesis of nalbuphine (VI) from oripavine (I) requires only five chemical transformations and is conducted with an overall yield of 45%, in contrast to current methods requiring seven to ten steps.
Local hierarchical control for industrial microgrids with improved frequency regulation (2018)

Local control strategies that operate without relying on communication systems enhance flexibility and reliability of AC industrial microgrids. Based on a previous work in which a secondary switched control was proposed, this paper presents a complementary strategy to improve the frequency regulation by reducing the maximum error. To this end, a dynamic-gain droop method driven by a time protocol is used. With this proposal, the maximum frequency error is effectively reduced without relying on complex techniques and maintaining the simplicity of the basis strategy and the non-use of communications. Experimental results obtained on a laboratory microgrid are presented to validate the performance of the proposed complementary control strategy.
Enantioselective synthesis of 2,6-dideoxy carbasugars based on a desymmetrizing hydroformylation–carbonyl ene cyclization process (2008)

A practical one-pot process involving a desymmetrizing hydroformylation with the aid of a chiral catalyst-directing group (CDG*), followed by a carbonyl ene cyclization provides a straightforward access to both enantiomers of the resulting cyclohexanediol; further divergent, highly selective and protecting group-free transformations furnish the carbocyclic analogues of four important 2,6-dideoxysugars.
A tunable photonic microwave notch filter using a self-injection locked reflective semiconductor optical amplifier (2009)

We propose a tunable photonic microwave notch filter using a self-injection locked reflective semiconductor optical amplifier (RSOA) and fiber Bragg gratings. Tunable filter characteristic is obtained by changing the center wavelength of one of the gratings. The achievable tuning range is determined by the gain profile of the RSOA and the tuning range of the grating.
Influence of Confinement on the Fragility of Antiplasticized and Pure Polymer Films (2006)

We investigate by molecular dynamics simulation how thin film confinement modifies the fragility of a model glass-forming liquid characterized previously in the bulk. Film confinement is found to reduce the relative fragility of the polymer fluid, leading to effects similar to simulations of the addition of an antiplasticizer additive. A reduction of fragility is not observed for the antiplasticized polymer film. These effects are interpreted in terms of variation in the string (cooperatively moving segments) concentration with film confinement and the addition of antiplasticizing additives.
Vacillantins A and B, New Anthrone C-glycosides, and a New Dihydroisocoumarin Glucoside from Aloe vacillans and Its Antioxidant Activities (2020)

A new dihydroisocoumarin glucoside, vacillanoside (<b>3</b>), and two new anthrone <i>C</i>-glycosides microdantin derivatives; vacillantin A (<b>10</b>) and B (<b>11</b>), together with nine known compounds belonging to the anthraquinone, anthrone and isocoumarin groups were isolated from the leaves of <i>Aloe vacillans</i>. The structures were determined based on spectroscopic evidence including 1D and 2D nuclear magnetic resonance (NMR) spectroscopy and high resolution mass spectrometry (HRESIMS) data, along with comparisons to reported data. The leaves were used to extract compounds with different solvents. The extracts were tested for antioxidant activity with a variety of in vitro tests including 2,2-diphenyl-1-picrylhydrazyl (DPPH<sup>•</sup>), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonate (ABTS<sup>•+</sup>), ferric reducing antioxidant power assay (FRAP), superoxide, and nitric oxide radical scavenging assays. The dichloromethane fraction was most active, displaying significant free radical scavenging activity. The <i>n</i>-butanol fraction also showed notable activity in all assays. Therefore, these findings support the potential use of <i>A. vacillans</i> leaves as an antioxidant medication due to the presence of polyphenolic compounds.