gebhart/patrepeval huggingface dataset:
Metallization of Silver Through Coffee‐Ring Assisted Ribonucleic Acid Scaffolding Technique (2019)

Abstract Preferential binding of base pair units with metallic ions within deoxyribonucleic acids (DNA) to form metal‐DNA complexes are well studied and understood. Excitingly, such natural processes have been utilized “artificially” for designing and fabricating nanometallic structures and patterns primarily using double stranded DNA. However, little or no effort has been given to ribonucleic acid or RNA in this aspect. Therefore, in this work we study the scaffolding effect of RNA on metalizing silver (Ag) ions and further the diffusion of majority of the Ag‐RNA complexes towards artificially introduced cut or scribed edges as a result of the coffee‐ring effect. Upon removal of the RNA scaffold, metallic nanostructures remain along the line of the cut corresponding to the micrometre scale length of the cut, exercising some amount of control and manipulation of parameter. Other parameters such as the height and diameter may directly be related to the concentration and diffusion of the Ag‐RNA complexes, while gap size related to the cut width. We anticipate that further in‐depth studies will be required before a comprehensive model could be proposed for highly controllable and flexible nanostructure and nanowire fabrication using nucleic acid scaffolding techniques.
Structure analysis by carbon-13 nuclear magnetic resonance spectroscopy of pandicine, a novel bisindole alkaloid from Pandacastrum saccharatum Pichon (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructure analysis by carbon-13 nuclear magnetic resonance spectroscopy of pandicine, a novel bisindole alkaloid from Pandacastrum saccharatum PichonChristiane Kan-Fan, Georges Massiot, Bhupesh C. Das, and Pierre PotierCite this: J. Org. Chem. 1981, 46, 7, 1481–1483Publication Date (Print):March 1, 1981Publication History Published online1 May 2002Published inissue 1 March 1981https://pubs.acs.org/doi/10.1021/jo00320a048https://doi.org/10.1021/jo00320a048research-articleACS PublicationsRequest reuse permissionsArticle Views93Altmetric-Citations22LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Refractive Indices of Ionic Liquids (2005)

At present, optical microscopy studies of minerals, especially diamonds, are hampered by the lack of available high refractive index (> 1.8) immersion fluids. We report here the syntheses and refractive indices of some 1-alkyl-3-methylimidazolium based ionic liquids containing polyhalide anions, which exhibit refractive indices between 1.6 and 2.23, and thus significantly extend the range of minerals which can be studied.
Analysis of vocal tremor in normophonic and dysphonic speakers (2015)

The study concerns the analysis of vocal cycle length perturbations in normophonic and dysphonic speakers.A method for tracking cycle lengths in voiced speech is proposed. The speech cycles are detected via the saliences of the speech signal samples, defined as the length of the temporal interval over which a sample is a maximum. The tracking of the cycle lengths is based on a dynamic programming algorithm that does not request that the signal is locally periodic and the average period length known a priori.The method is validated on a corpus of synthetic stimuli. The results show a good agreement between the extracted and the synthetic reference length time series. The method is able to track accurately low-frequency modulations and ast cycle-to-cycle perturbations of up to 10% and 4% respectively over the whole range of vocal frequencies. Robustness with regard to the background noise has lso been tested. The results indicate that the tracking is reliable for signal-to-noise ratios higher than 15dB.A method for analyzing the size of the cycle length perturbations as well as their frequency is proposed. The cycle length time series is decomposed into a sum of oscillating components by empirical mode decomposition the instantaneous envelopes and frequencies of which are obtained via AM-FM decomposition. Based on their average instantaneous frequencies, the empirical modes are then assigned to four categories (declination, physiological tremor, neurological tremor as well as cycle length jitter) and added within each. The within-category size of the cycle length perturbations is estimated via the standard deviation of the empirical mode sum divided by the average cycle length. The neurological tremor modulation frequency and bandwidth are obtained via the instantaneous frequencies and amplitudes of empirical modes in the neurological tremor category and summarized via a weighted instantaneous frequency probability density, compensating for the effects of mode mixing.The method is applied to two corpora of vowels comprising 123 and 74 control and 456 and 205 Parkinson speaker recordings respectively. The results indicate that the neurological tremor modulation depth is statistically significantly higher for female Parkinson speakers than for female control speakers. Neurological tremor frequency differs statistically significantly between male and female speakers and increases statistically significantly for the pooled Parkinson speakers compared to the pooled control speakers. Finally, the average vocal frequency increases for male Parkinson speakers and decreases for female Parkinson speakers, compared to the control speakers.
Optomechanical nanoresonator readout with optical downmixing (2016)

We report an optomechanical downmixing measurement method for nanomechanical resonators, which enables the readout of resonance frequencies larger than the bandwidth of the photodetector. We show this method can easily readout the Brownian noise of the device, in which case the performance of the system is not degraded with respect to direct measurement. In the case when the performance is limited by the photodetector, this technique allows employing low-bandwidth/low-noise photodetectors, leading to higher resolution for sensing applications. This is key in particular for mass spectrometry applications where both high displacement sensitivity and multi-mode operation are required.
Report of the First National Lidar Initiative Meeting, February 14-16, Reston, Va. (2007)

The first National Lidar Initiative meeting was held on February 14-16, 2007 at the USGS National Center in Reston, Virginia. This meeting was a successor to a meeting held September 12, 2006 of several agencies, including the National Aeronautics and Space Administration (NASA), the Association of American State Geologists (AASG), and the U.S. Geological Survey (USGS). During the 2006 discussion, the USGS presented a plan to organize a meeting to discuss the feasibility and strategy of a National Lidar Initiative. Originally scheduled as a workshop to be held in June, 2007, the meeting was moved up to February to accommodate the desire of AASG to have talking points available at their Annual Meeting in March, 2007. The original workshop was recast as a meeting of representatives from Federal, State, and local government, and from private industry, to formulate a national initiative with the goal of collecting high resolution, high accuracy light detection and ranging (lidar) data for all 50 states. The goals of the National Lidar Initiative meeting were to: 1) Identify government staff with all scales of applications who are willing to help devise a potential national strategy and communicate the initiative throughout the lidar community. 2) Identify points of contact for future meetings, information exchanges, and design teams. 3) Compose a document explaining the need of a consistent national lidar dataset. 4) Identify champions of this idea who would be willing to work toward funding this effort. This report is intended to summarize the views expressed by the invited speakers and the participant's discussions on a National Lidar Initiative. It is not intended to be a comprehensive document on the technical aspects of lidar, what lidar can be used for, or the state of the art in lidar technology, although many of these aspects do come through in the views of the presenters.
Nucleophilic substitution of (alkoxymethylene)dimethylammonium chloride with potassium phthalimide; a convenient procedure for the synthesis of imides with inversion of configuration (1997)

A. G. M. Barrett, D. Christopher Braddock, R. A. James and P. A. Procopiou, Chem. Commun., 1997, 433 DOI: 10.1039/A607482K
A distributed edge detection and surface reconstruction algorithm (2002)

A scalable parallel algorithm for edge detection and surface reconstruction is presented. The algorithm is based on fitting a weak membrane to the pixel gray valves by minimizing the associated energy functional. The edge detection process is modeled as a line process and used as a constraint in minimizing the energy functional of the image. The optimal edge assignment cannot be obtained directly as the energy function is non-convex. Using graduated non-convexity (GNC) approach, the energy is minimized. The proposed parallel algorithm has been implemented on a cluster of workstations using the PVM communication library. The results of parallel implementation on synthetic and natural images are presented. The speedup is observed to be near-linear, thus providing scalability with the problem size. The parallel processing approach presented here can be extended to solve similar problems (e.g., image restoration, and image compression) which use regularization techniques.
An accurate approximate algorithm for motion compensation in two-dimensional tomography (2010)

In this paper, we propose two approximate inversion formulae for motion compensation in tomography: for parallel beam and fan beam geometries. Let E denote the operator, which corresponds to the error term of an inversion formula. It is proven that in both cases E: Hm0 → Hm+10 is bounded; thus, the error term is one order smoother than the original function f in the scale of Sobolev spaces. It is also proven that in both cases if themotionmap approaches the identity map, then the norm of E approaches zero. The formulae can be easily implemented numerically. Results of numerical experiments in the fanbeam case (which is more common in applications) demonstrate good image quality even when motion is relatively strong. © 2010 IOP Publishing Ltd.
A novel experimental rat model of peripheral nerve scarring: reliably mimicking post-surgical complications and recurring adhesions (2017)

Inflammation, fibrosis and perineural adhesions with the surrounding tissue are common pathological processes following nerve injury and surgical interventions on peripheral nerves in human patients. These features can reoccur following external neurolysis, currently the most common surgical treatment for peripheral nerve scarring, thus leading to renewed nerve function impairment and chronic pain. To enable a successful evaluation of new therapeutic approaches, it is crucial to use a reproducible animal model that mimics the main clinical symptoms occurring in human patients. However, a clinically relevant model combining both histological and functional alterations has not been published to date. We therefore developed a reliable rat model that exhibits the essential pathological processes of peripheral nerve scarring. In our study, we present a novel method for the induction of nerve scarring by applying glutaraldehyde-containing glue that is known to cause nerve injury in humans. After a 3-week contact period with the sciatic nerve in female Sprague Dawley rats, we could demonstrate severe intra- and perineural scarring that resulted in grade 3 adhesions and major impairments in the electrophysiological peak amplitude compared with sham control (<i>P</i>=0.0478). Immunohistochemical analysis of the nerve structure revealed vigorous nerve inflammation and recruitment of T cells and macrophages. Also, distinct nerve degeneration was determined by immunostaining. These pathological alterations were further reflected in significant functional deficiencies, as determined by the analysis of relevant gait parameters as well as the quantification of the sciatic functional index starting at week 1 post-operation (<i>P</i><0.01). Moreover, with this model we could, for the first time, demonstrate not only the primary formation, but also the recurrence, of severe adhesions 1 week after glue removal, imitating a major clinical challenge. As a comparison, we tested a published model for generating perineural fibrotic adhesions, which did not result in significant pathological changes. Taken together, we established an easily reproducible and reliable rat model for peripheral nerve scarring that allows for the effective testing of new therapeutic strategies.
Multiple inputs control sulfur-containing amino acid synthesis in<i>Saccharomyces cerevisiae</i> (2014)

In Saccharomyces cerevisiae, transcription of the MET regulon, which encodes the proteins involved in the synthesis of the sulfur-containing amino acids methionine and cysteine, is repressed by the presence of either methionine or cysteine in the environment. This repression is accomplished by ubiquitination of the transcription factor Met4, which is carried out by the SCF(Met30) E3 ubiquitin ligase. Mutants defective in MET regulon repression reveal that loss of Cho2, which is required for the methylation of phosphatidylethanolamine to produce phosphatidylcholine, leads to induction of the MET regulon. This induction is due to reduced cysteine synthesis caused by the Cho2 defects, uncovering an important link between phospholipid synthesis and cysteine synthesis. Antimorphic mutants in S-adenosyl-methionine (SAM) synthetase genes also induce the MET regulon. This effect is due, at least in part, to SAM deficiency controlling the MET regulon independently of SAM's contribution to cysteine synthesis. Finally, the Met30 protein is found in two distinct forms whose relative abundance is controlled by the availability of sulfur-containing amino acids. This modification could be involved in the nutritional control of SCF(Met30) activity toward Met4.
Evaluation of Therapeutic Collagen-Based Biomaterials in the Infarcted Mouse Heart by Extracellular Matrix Targeted MALDI Imaging Mass Spectrometry (2021)

The goal of this study was to develop strategies to localize human collagen-based hydrogels within an infarcted mouse heart, as well as analyze its impact on endogenous extracellular matrix (ECM) remodeling. Collagen is a natural polymer that is abundantly used in bioengineered hydrogels because of its biocompatibility, cell permeability, and biodegradability. However, without the use of tagging techniques, collagen peptides derived from hydrogels can be difficult to differentiate from the endogenous ECM within tissues. Imaging mass spectrometry is a robust tool capable of visualizing synthetic and natural polymeric molecular structures yet is largely underutilized in the field of biomaterials outside of surface characterization. In this study, our group leveraged a recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) technique to enzymatically target collagen and other ECM peptides within the tissue microenvironment that are both endogenous and hydrogel-derived. Using a multimodal approach of fluorescence microscopy and ECM-IMS techniques, we were able to visualize and relatively quantify significantly abundant collagen peptides in an infarcted mouse heart that were localized to regions of therapeutic hydrogel injection sites. On-tissue MALDI MS/MS was used to putatively identify sites of collagen peptide hydroxyproline site occupancy, a post-translational modification that is critical in collagen triple helical stability. Additionally, the technique could putatively identify over 35 endogenously expressed ECM peptides that were expressed in hydrogel-injected mouse hearts. Our findings show evidence for the use of MALDI-IMS in assessing the therapeutic application of collagen-based biomaterials.
Evaluating Solvothermal and Mechanochemical Routes towards the Metal–Organic Framework Mg2(m-dobdc) (2022)

Metal–organic frameworks bearing coordinatively unsaturated Mg(II) sites are promising materials for gas storage, chemical separations, and drug delivery due to their low molecular weights and lack of toxicity. However, there remains a limited number of such MOFs reported in the literature. Herein, we investigate the gas sorption properties of the understudied framework Mg2(m-dobdc) (dobdc4− = 4,6-dioxido-1,3-benzenedicarboxylate) synthesized under both solvothermal and mechanochemical conditions. Both materials are found to be permanently porous, as confirmed by 77 K N2 adsorption measurements. In particular, Mg2(m-dobdc) synthesized under mechanochemical conditions using exogenous organic base displays one of the highest capacities reported to date (6.14 mmol/g) for CO2 capture in a porous solid under simulated coal flue gas conditions (150 mbar, 40 °C). As such, mechanochemically synthesized Mg2(m-dobdc) represents a promising new framework for applications requiring high gas adsorption capacities in a porous solid.
Silver Space-Time Trellis Coded Modulation (2008)

Publication in the conference proceedings of EUSIPCO, Lausanne, Switzerland, 2008
Essential Oil Constituents of<i>Ocimum kilimandscharicum</i>Guerke (1992)

ABSTRACT The volatile oil of Ocimum kilimandscharicum Guerke, a perennial undershrub of Labiatae, grown in Indiana was analyzed by GC and GC/MS. The essential oil content varied between the leaves (0.77–1.12% dry wt. basis) and the flowers (1.96–2.8% dry wt. basis). Seventeen constituents were identified in the oil. Oil composition was similar between the leaves and flowers with linalool as the major constituent (leaves (41.94%), flowers (58.85%)). Other major constituents included camphor (leaves (17.02%), flowers (15.82%)) and 1,8-cineole (leaves (10.18%), flowers (6.38%)). We report a ‘linalool-camphor’ chemotype of O. kilimandscharicum.
Development of airborne/satellite lidars using non linear optics for characterization of greenhouse gases (2016)

Active optical sensing of the atmosphere through long distances from airborne or satellite platforms requires specific and demanding features for the necessary laser sources in terms of wavelength addressing and power level. Many molecules of atmospheric interest for nowadays urgent issues like climate change and contrails (CO <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</inf> , CH <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">4</inf> , H <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</inf> O) have their best relevant detection features in the near and mid infrared spectral bands whereas most present days robust lasers have limited emission in the visible or near infrared at a few fixed wavelengths imposed by the laser media. Photonic solutions like DFG, OPO or Raman shift have to be employed to provide the necessary frequency conversion to cover the spectral fingerprints of molecules to be probed. This presentation will review some of the developments undertaken in our laboratory to provide attractive solutions using these schemes. One of promising solutions is a high energy transmitter around 2 µm based on frequency conversion in a nested cavity doubly resonant optical parametric oscillator (NesCOPO) followed by high energy parametric amplification (MOPA architecture) as depicted in Figure1. This scheme enables the generation of tunable single-frequency (< 60 MHz) high energy nanosecond pulses (tens of mJ) suitable for differential absorption lidar (DIAL) probing of the atmosphere (Figure 2). Development is also being carried out with picosecond/femtosecond frequency comb lasers to provide extended spectral coverage for more species and to enable detection schemes like Fourier transform spectroscopy with a dual comb. Ultra-sensitive trace level local probing on a balloon or airplane carrier is also being investigated through enhanced geometry for quartz-enhanced photoacoustic spectroscopy.
New rat model of Pneumocystis carinii infection (1988)

Rats free of latent Pneumocystis carinii organisms were immunosuppressed with adrenal corticosteroids and transtracheally injected with P. carinii. These animals subsequently developed P. carinii pneumonia. Infection was accomplished by using organisms from infected rat lung or from culture. Diffuse infection was produced with no significant differences in the numbers of organisms found in various lobes of the lungs. Infections progressed over time so that by 6 weeks postinoculation all animals were heavily infected. Infection by transtracheal injection has three advantages over current models. First, transtracheal injection provides a reliable model which is not dependent on naturally occurring latent Pneumocystis infection. Second, transtracheal injection allows the perpetuation of specific Pneumocystis strains. Third, transtracheal injection is a more rapid and economical means of producing severe Pneumocystis pneumonia.
Gene mapping and cross-resistance in cyclodiene insecticide-resistant <i>Drosophila melanogaster</i> (Mg.). (1991)

Resistance to the cyclodiene insecticide dieldrin maps to a single gene (Rdl) on the left arm of chromosome III in Drosophila melanogaster (Meigen). The gene was further mapped by the use of chromosomal deficiencies to a single letter sub-region, 66F, on the polytene chromosome. The cross-resistance spectrum of a backcrossed strain lacking elevated mixed function oxidase activity, a common resistance mechanism, was examined. Levels of resistance similar to those found in other insects were found to dieldrin, aldrin, endrin, lindane, and picrotoxinin. Strong similarity of this single major gene with that found in other cyclodiene resistant insects is suggested by its cross-resistance spectrum and chromosomal location, via homology with other Diptera. The significance of major genes in insecticide resistance is discussed.
Sliding mode PWM for effective current control in Switched Reluctance Machine drives (2014)

In this paper the problem of effective current control in Switched Reluctance Machine (SRM) drives is addressed. A sliding mode pulse width modulator (SMPWM) is introduced to govern the inherently highly nonlinear system dynamics. The proposed SRM drive is analyzed theoretically and subsequently validated through simulation. Satisfactory dynamic performance and precise speed control with reduced torque ripple, uniform power electronic device utilization, and approximately constant switching frequency is obtained. The feasibility of the above control scheme is validated by experimental measurements on a prototype 6/4 SRM drive.