gebhart/patrepeval huggingface dataset:
Orbital angular momentum modes by twisting of a hollow core antiresonant fiber (2017)

Generation and use of orbital angular momentum (OAM) of light is finding more and more interest in a wide variety of fields of photonics: communications, optical trapping, quantum optics, and many more. In the investigation of such behavior, twisting of photonic crystal fibers shows interesting physical phenomena. We previously reported the ability to create helical hollow fibers by mechanically twisting a tube lattice fiber made of polyurethane, the twist of which can be adjusted and reversed. In this work we report how such deformation induces a mode transformation to an OAM mode, allowing a simple and tunable way to generate OAM modes. We take advantage of THz time domain spectroscopy to obtain information on both intensity and field components, and to be able to investigate how they change both in time and with frequency.
Efficacy and safety evaluation of claudin‐4‐targeted antitumor therapy using a human and mouse cross‐reactive monoclonal antibody (2016)

Claudin-4 (CLDN-4), a tight-junction protein, is overexpressed in various malignant tumors, including gastric, colorectal, pancreatic, and breast cancers. However, CLDN-4 is also expressed in normal tissues, including the liver, pancreas, kidney, and small intestine. Whether CLDN-4 is an effective and safe target for cancer therapy has been unclear owing to the lack of a binder with both CLDN-4 specificity and cross-reactivity to human and murine cells. In this study, we successfully generated a rat anti-CLDN-4 monoclonal antibody (5D12) that was specific to, and cross-reactive with, human and mouse CLDN-4. 5D12 recognized the second extracellular domain of human CLDN-4 in a conformation-dependent manner. A human-rat chimeric IgG1 of 5D12 (xi-5D12) activated the Fc<i>γ</i> IIIa receptor, indicating the activation of antibody-dependent cellular cytotoxicity in CLDN-4-expressing cells. Moreover, xi-5D12 significantly suppressed tumor growth in mice bearing human colorectal and gastric tumors without apparent adverse effects, such as weight loss or liver and kidney damage. These results suggest that CLDN-4 is a potent target for cancer therapy and that an anti-CLDN-4 antibody is a promising candidate anticancer agent.
Evaluating Future Electrified UAM-Enabled Middle-Mile Cargo Delivery Operations (2022)

View Video Presentation: https://doi.org/10.2514/6.2022-3756.vid The emergence of novel electric aerospace systems in Advanced Air Mobility (AAM) has enabled the research and development of new opportunities for innovative mission profiles. One such mission is Middle-Mile Delivery (MMD) which seeks to use Urban Air Mobility (UAM) vehicles to transport cargo between distribution centers or facilities in a cargo operator’s network. This paper quantitatively analyzes future operations and limits of UAM-enabled MMD through developing a methodology for package demand generation and modal choice for UAM cargo MMD missions. Case studies of cargo deliveries in the Chicago metropolitan area highlight the computational framework developed for MMD modal choice analysis.
023 * FIRST RESULTS OF 111 MINIMALLY-INVASIVE LEFT VENTRICULAR ASSIST DEVICE IMPLANTATIONS AT A SINGLE-CENTRE (2014)

Objectives: Left ventricular assist devices (LVADs) are gaining more importance in the treatment of terminal heart failure. The development of miniaturised technologies has generated considerably novel LVAD systems enabling less traumatic implantation procedures with the potential of lowering perioperative risk. So far, clinical experience with minimally-invasive LVAD surgery was limited to only a few experienced centres. In this study, we elucidate the operative outcome of the first series of 100 patients who received minimally-invasive LVAD surgery at a single institution. Methods: We reviewed the outcome of 111 end-stage heart failure patients who underwent minimally-invasive LVAD implantation (HVAD, HeartWare) in our institution between 2011 and 2013. Patients were operated using the “Hannover”-LVAD-technique: upper hemisternotomy and combined with a left-sided anterolateral thoracotomy. Results: Between 2011 and 2014, 111 minimally-invasive LVAD implantations were performed at our institution (78% male, 22% female; mean age 52 ± 4; dilated cardiomyopathy (DCM) 42.6%; ischaemic cardiomyopathy (ICM) 44.4%, other aetiologies 13%). Thirty-day mortality was revealed to be 5.2%, 90-day mortality 9.0% and 1-year survival 88%). Compared to literature and historical data of conventional LVAD implantations, patients who were operated minimally-invasively showed a lower bleeding incidence (9.6%), which is also indicated by lower amounts of applied packed red blood cells (mean 4.9 rbc), shorter mean ICU stay (9.3 days), and a lower incidence of right heart failure (4.6%). Conclusion: Minimally-invasive LVAD implantations are proven to be safe and associated with a lower perioperative complication and mortality rate. By using this technique (upper hemisternotomy plus anterolateral thoracotomy) the intra-hospital outcome was significantly improved (as especially indicated by lower bleeding incidences and lower right heart failure rates) compared to patients treated by the standard surgical technique.
An in vitro study of composites of poly(L-lactide-co-ε-caprolactone), β-tricalcium phosphate and ciprofloxacin intended for local treatment of osteomyelitis (2012)

Osteomyelitis is a bacterial disease that can become chronic, and treatment often includes a surgical operation to remove infected bone. The aim of this study was to develop and investigate in vitro bone filling composite materials that release ciprofloxacin to kill any remaining bacteria and contain bioceramic to help the bone to heal. Three composites of poly(L-lactide-co-ε-caprolactone), β-tricalcium phosphate and ciprofloxacin were compounded using twin-screw extrusion and sterilized by gamma irradiation. Drug release and degradation of the composites were investigated in vitro for 52 weeks. The composite with 50 wt% of β-TCP had the most promising ciprofloxacin release profile. The ceramic component accelerated the drug release that occurred in three phases obeying first-order kinetics. Inhibition zone testing using bioluminescence showed that the released ciprofloxacin had effect in eradicating a common osteomyelitis causing bacteria Pseudomonas aeruginosa. During the in vitro degradation test series, molar weight of the polymer matrix of the composites decreased rapidly. Additionally, (1)H-NMR analysis showed that the polymer had blocky structure and the comonomer ratio changed during hydrolysis. The tested composites showed great potential to be developed into bone filler materials for the treatment of osteomyelitis or other bone related infections.
Comparison of Energy Efficiencies of Commercial Refrigeration Direct and Indirect Systems (1998)

Centralized commercial refrigeration is becoming a sector where several technical options are competitive.These options are evaluated based on three major criterions: energy consumption, refrigerant emissions and initial cost.In supermarkets with machinery rooms, the competition is strong between previous centralized direct systems and new systems using heat transfer fluids.To achieve balanced comparisons between energy efficiency of these competitive techniques, measurements of direct expansion systems and of systems using monophase heat transfer fluids have been performed on site.
Temperature-Modulated Interaction Changes with Adenosine Nucleotides on Intelligent Cationic, Thermoresponsive Surfaces1 (2007)

Thin layer poly(N-isopropylacrylamide-co-n-butyl-methacrylate-co-N,N-dimethylaminopropylacrylamide) (IBD) copolymer gels are covalently introduced to initiator immobilized silica bead surfaces to create thermally sensitive intelligent cationic surfaces. The surface shows thermoresponsive changes in charge density as well as hydrophilic/hydrophobic character. The polymer chains dehydrate and inter-/intra-molecular aggregation occurs due to weakly deprotonated cationic amino groups in the hydrophobized circumstances, resulting in the surface charge density decrease. This was corroborated by the thermoresponsive pKa shift of dimethylamino side groups in the copolymers as well as the surface potential changes at elevated temperature. The unique characteristics of the IBD copolymer-immobilized surfaces were applied to regulate adenosine nucleotides retention in high-performance liquid chromatography using aqueous mobile phase by thermal stimulus. At lower temperature, adenosine nucleotides showed higher retention which was primarily driven by ionic interaction with positively charged surfaces. With increasing temperatures, their retention was shortened and a drastic change was observed above the polymer transition temperatures. This is strong evidence that the solute interaction is being regulated by the thermoresponsive surface charge density changes and hydrophobic alterations. Furthermore, we confirmed the modulation of nucleotide retention by step-temperature gradient without changing mobile phase composition. These findings should be beneficial in utilizing this stimuli responsive surface for the separation of bioactive compounds in aqueous system and environmental impact.
Toll-Like Receptor mRNA Expression Is Selectively Increased in the Colonic Mucosa of Two Animal Models Relevant to Irritable Bowel Syndrome (2009)

These data suggest that both early life stress (MS) and a genetic predisposition (WKY) to stress affect the expression of key sentinels of the innate immune system which may have direct relevance for the molecular pathophysiology of IBS.
Knee Challenges: What Would You Do? (1997)

Case Challenges In Hip and Knee Arthroplasty
Repair of Lacerated Peripheral Nerves With Nerve Conduits (2008)

Peripheral nerve lesions are relatively common injuries encountered by hand surgeons. These injuries are notorious for causing significant and potentially long-standing impairment to hand function. Numerous surgical techniques with varying degrees of success have been described to treat this injury. The evolution of peripheral nerve repair has led to the development of the nerve conduit, a surgical technique that functionally bridges the gap between transected nerves. We discuss a brief history and evolution of nerve conduits and offer our preferred technique for peripheral nerve repair with a collagen nerve conduit. In addition, we offer case studies and postoperative rehabilitation goals and present early results associated with this type of repair.
Identification, isolation, and N-terminal sequencing of style glycoproteins associated with self-incompatibility in Nicotiana alata. (1989)

S-Gene-associated glycoproteins (S-glycoproteins) from styles of Nicotiana alata, identified by non-equilibrium two-dimensional electrophoresis, were purified by cation exchange fast protein liquid chromatography with yields of 0.5 to 8 micrograms of protein per style, depending on the S-genotype of the plant. The method relies on the highly basic nature of the S-glycoproteins. The elution profiles of the different S-glycoproteins from the fast protein liquid chromatography column were characteristic of each S-glycoprotein, and could be used to establish the S-genotype of plants in outbreeding populations. In all cases, the S-genotype predicted from the style protein profile corresponded to that predicted from DNA gel blot analysis using S-allele-specific DNA probes and to that established by conventional breeding tests. Amino-terminal sequences of five purified S-glycoproteins showed a high degree of homology with the previously published sequences of N. alata and Lycopersicon esculentum S-glycoproteins.
Impact of molecular tumor board on late-stage gastrointestinal malignancies at a tertiary center. (2018)

483 Background: Inova Schar Cancer Institute formed a molecular tumor board in early 2016 to identify treatment options for patients with gastrointestinal malignances based on molecular testing and to track outcomes. Methods: From March 2016 to June 2017, 78 patients with advanced gastrointestinal malignancies were presented at our molecular tumor board. The most common mutations, the percentage of patients who received targeted therapies, responded to targeted therapies, died or went on hospice prior to receiving a recommended therapy, and had an available Association of Molecular Pathology Tier 1 or Tier 2 recommendation available were reviewed retrospectively. We also compared the overall survival of patients who received a new treatment after MTB compared to those who did not. N-of-One, Inc. provided curation of molecular testing and participated in the MTB. Results: 78 patients with gastrointestinal cancers were presented between March 2016 and June 2017. Thirteen (19%) patients received targeted therapy and 31% had partial response, 15% had Stable Disease, and 54% had progression of disease. 12 patients (15%) died or went on hospice before recommendations could start and 11 patients (14%) are waiting to start recommended therapy. 38 (49%) patients did not have a mutation that prompted a MTB recommendation. Median OS of the 33 patients who started a new therapy (including chemotherapy or unrelated clinical trials) after MTB was 15.3 months vs. 11.5 months in 40 patients who continued current therapy (P = 0.016 Wilcoxon). The three most common mutations detected were TP53, KRAS, and APC. The majority of cases had more than one variant. 18% of cases had a variant classified as Tier 1; 74% of cases had a variant with the highest AMP classification of Tier 2. Conclusions: The majority of patients with Gastrointestinal malignancies presented to the Inova MTB had a finding that supported a molecularly-guided therapy, with a small but meaningful number of partial responses. Barriers to the use of molecular guided therapy included molecular testing and presentation at a late disease stage, and alternative chemotherapeutic options.
ChemInform Abstract: Oxygen Atom Insertion into the Benzylic Carbon‐Hydrogen Bond of (R)‐(‐) ‐2‐Phenylbutane (I) by Methyl(trifluoromethyl)dioxirane: An Efficient and Mild Regio‐ and Stereoselective Synthesis of (S)‐(‐)‐2‐Phenyl‐2‐ butanol (II). (1992)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: PHOSPHORUS DOPING OF LOW PRESSURE CHEMICALLY VAPOR‐DEPOSITED SILICON FILMS (1979)

Abstract Das Leitfähigkeitsverhalten von polykristallinen Si‐Filmen, die bei 580 und 620°C und niedrigen Drücken (0.5 Torr) chemisch aus der Gasphase (thermische Zers. von Silan) abgeschieden werden und durch Ionenimplantation mit Pdotiert werden, wird mit dem von unter Atmosphärendruck abgeschiedenen Filmen verglichen.
Complement activation impacts B-cell depletion by both type I and type II CD20 monoclonal antibodies (2008)

These results emphasize potential contribution of the genetic background of HCV-infected patients in the development of LPDs. In this light, specific human leukocyte antigen (HLA) clusters have been previously associated with a higher risk of developing MCS and concomitant NHL. 9 In addition, recent data limiting the importance of virus-specific determinants are consistent with the relevance of genetic and host factors in promoting HCV-related LPDs. 10 The transcriptional activation induced by the mutated BAFF promoter can be considered one of the mechanisms involved in the pathogenesis of HCV-related autoimmune/lymphoproliferative disorders, and the polymorphism can contribute, possibly in combination with other allelic patterns, to determining a genetic profile characteristic of the cryoglobulinemic phenotype.
Mechanism of De Novo Initiation by the Hepatitis C Virus RNA-Dependent RNA Polymerase: Role of Divalent Metals (2002)

We functionally analyzed the role of metal ions in RNA-dependent RNA synthesis by three recombinant RNA-dependent RNA polymerases (RdRps) from GB virus-B (GBV), bovine viral diarrhea virus (BVDV), and hepatitis C virus (HCV), with emphasis on the HCV RdRp. Using templates capable of both de novo initiation and primer extension and RdRps purified in the absence of metal, we found that only reactions with exogenously provided Mg(2+) and Mn(2+) gave rise to significant amounts of synthesis. Mg(2+) and Mn(2+) affected the mode of RNA synthesis by the three RdRps. Both metals supported primer-dependent and de novo-initiated RNA by the GBV RdRp, while Mn(2+) significantly increased the amount of de novo-initiated products by the HCV and BVDV RdRps. For the HCV RdRp, Mn(2+) reduced the K(m) for the initiation nucleotide, a GTP, from 103 to 3 micro M. However, it increased de novo initiation even at GTP concentrations that are comparable to physiological levels. We hypothesize that a change in RdRp structure occurs upon GTP binding to prevent primer extension. Analysis of deleted proteins revealed that the C terminus of the HCV RdRp plays a role in Mn(2+)-induced de novo initiation and can contribute to the suppression of primer extension. Spectroscopy examining the intrinsic fluorescence of tyrosine and tryptophan residues in the HCV RdRp produced results consistent with the protein undergoing a conformational change in the presence of metal. These results document the fact that metal can affect de novo initiation or primer extension by flaviviral RdRps.
Integration of modeling with experimental and clinical findings synthesizes and refines the central role of inositol 1,4,5-trisphosphate receptor 1 in spinocerebellar ataxia (2015)

A suite of models was developed to study the role of inositol 1,4,5-trisphosphate receptor 1 (IP3R1) in spinocerebellar ataxias (SCAs). Several SCAs are linked to reduced abundance of IP3R1 or to supranormal sensitivity of the receptor to activation by its ligand inositol 1,4,5-trisphosphate (IP3). Detailed multidimensional models have been created to simulate biochemical calcium signaling and membrane electrophysiology in cerebellar Purkinje neurons. In these models, IP3R1-mediated calcium release is allowed to interact with ion channel response on the cell membrane. Experimental findings in mice and clinical observations in humans provide data input for the models. The SCA modeling suite helps interpret experimental results and provides suggestions to guide experiments. The models predict IP3R1 supersensitivity in SCA1 and compensatory mechanisms in SCA1, SCA2, and SCA3. Simulations explain the impact of calcium buffer proteins. Results show that IP3R1-mediated calcium release activates voltage-gated calcium-activated potassium channels in the plasma membrane. The SCA modeling suite unifies observations from experiments in a number of SCAs. The cadre of simulations demonstrates the central role of IP3R1.
Experimental metrology beyond the standard quantum limit for a wide resources range (2023)

Abstract Adopting quantum resources for parameter estimation discloses the possibility to realize quantum sensors operating at a sensitivity beyond the standard quantum limit. Such an approach promises to reach the fundamental Heisenberg scaling as a function of the employed resources N in the estimation process. Although previous experiments demonstrated precision scaling approaching Heisenberg-limited performances, reaching such a regime for a wide range of N remains hard to accomplish. Here, we show a method that suitably allocates the available resources permitting them to reach the same power law of Heisenberg scaling without any prior information on the parameter. We demonstrate experimentally such an advantage in measuring a rotation angle. We quantitatively verify sub-standard quantum limit performances for a considerable range of N (O(30,000)) by using single-photon states with high-order orbital angular momentum, achieving an error reduction, in terms of the obtained variance, &gt;10 dB below the standard quantum limit. Such results can be applied to different scenarios, opening the way to the optimization of resources in quantum sensing.
High throughput screening using enzyme assay microarrays (2002)

We report a slide based microarray platform for assaying multiple enzyme activities using fluorogenic substrates. The method enables us to achieve the microfluidic requirements for rapid reaction assembly and compartmentalization. We can thus determine enzymatic activities in individually controlled reaction environments containing cofactors, inhibitors and activators. Fluorogenic substrates in glycerol were arrayed onto glass slides with reaction volumes < 5 nL and feature sizes of <150 /spl mu/m. Our method allowed rapid multiple sample deliveries onto the slide (<3 nL/spot) with no cross contamination between array positions. It enabled us to detect the activation of the fibrinolytic and coagulation proteases namely, thrombin, plasmin, factor Xa, tPa and kallikrein in human plasma. Enzyme-substrate-inhibitor assays using ten caspases were also performed. With over 400 spots/cm/sup 2/, combinatorial substrate libraries with different proteases can now be rapidly profiled. An assay to detect the dose response of a thrombin inhibitor benzamidine was performed. The inhibitor was arrayed in replicates onto selected positions on the chip. After sequential subnanoliter delivery of the reaction components, the result from the array was analyzed. The expected dose response from benzamidine was seen. A CV of 5.26% was achieved for 232 positions on the array not spiked with the inhibitor. Thus, with potentially several thousand compounds per slide, using rapid sub-nanoliter delivery of components and standard equipment, the true potential of the method is in the field of high throughput screening.
Syntheses and NMR and XRD studies of carbohydrate–ferrocene conjugates (2019)

Carbohydrate–ferrocene conjugates show a splitting of all the <sup>1</sup>H NMR signals of the substituted cyclopentadienyl. XRD of crystals seems to indicate that the R group could be responsible for the splitting of the NMR signals.
New and Convenient Synthesis of Lithiated Allenes and Ketenimines Based on Readily Available Aryl-Substituted Ketones and Amides (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNew and Convenient Synthesis of Lithiated Allenes and Ketenimines Based on Readily Available Aryl-Substituted Ketones and AmidesDietmar Seyferth, Peter Langer, and Manfred DoeringCite this: Organometallics 1995, 14, 10, 4457–4459Publication Date (Print):October 1, 1995Publication History Published online1 May 2002Published inissue 1 October 1995https://pubs.acs.org/doi/10.1021/om00010a006https://doi.org/10.1021/om00010a006research-articleACS PublicationsRequest reuse permissionsArticle Views371Altmetric-Citations16LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts