gebhart/patrepeval huggingface dataset:
Fabrication of low-fouling, high-loading polymeric surfaces through pH-controlled RAFT (2020)

Low-fouling and high-loading surfaces are increasingly important for biosensing and blood purification technologies. Selective and efficient target binding from complex media can be achieved with poly(carboxybetaine) (pCB) surfaces that consist of a dense brush layer to resist non-specific protein adsorption and a sparse "mushroom" upper layer for high-density capture agent immobilization (<i>i.e.</i> high-loading). We developed pH-controlled surface-reversible addition-fragmentation chain-transfer (S-RAFT) polymerization to simplify fabrication of multi-modal, low-fouling and high-loading pCB surfaces without the need for quenching or re-initiation steps, toxic transition metals or light irradiation. Multi-modal polymer layers were produced through partial polymer termination by temporarily raising the pH to aminolyse a fraction of dormant chain transfer agents (CTAs); remaining polymer chains with intact CTAs continued uninterrupted extension to create the "mushroom" upper layer. The multi-modal pCB surfaces were low-fouling towards proteins (<6.7 ng cm<sup>-2</sup>), and macrophages. Compared to mono-modal brush surfaces, multi-modal pCB surfaces were high-loading with 5-fold greater capture agent immobilization (<i>e.g.</i> antibody) and 4-fold greater target binding (<i>e.g.</i> biotin-fluorescein).
Enhanced Production of Green Tide Algal Biomass through Additional Carbon Supply (2013)

Intensive algal cultivation usually requires a high flux of dissolved inorganic carbon (Ci) to support productivity, particularly for high density algal cultures. Carbon dioxide (CO2) enrichment can be used to overcome Ci limitation and enhance productivity of algae in intensive culture, however, it is unclear whether algal species with the ability to utilise bicarbonate (HCO3 (-)) as a carbon source for photosynthesis will benefit from CO2 enrichment. This study quantified the HCO3 (-) affinity of three green tide algal species, Cladophora coelothrix, Cladophora patentiramea and Chaetomorpha linum, targeted for biomass and bioenergy production. Subsequently, we quantified productivity and carbon, nitrogen and ash content in response to CO2 enrichment. All three species had similar high pH compensation points (9.7-9.9), and grew at similar rates up to pH 9, demonstrating HCO3 (-) utilization. Algal cultures enriched with CO2 as a carbon source had 30% more total Ci available, supplying twenty five times more CO2 than the control. This higher Ci significantly enhanced the productivity of Cladophora coelothrix (26%), Chaetomorpha linum (24%) and to a lesser extent for Cladophora patentiramea (11%), compared to controls. We demonstrated that supplying carbon as CO2 can enhance the productivity of targeted green tide algal species under intensive culture, despite their clear ability to utilise HCO3 (-).
Discover Archaeology: An Introduction to the Tools and Techniques of Archaeological Fieldwork. George Sullivan Penguin Books, New York, 1980. ix + 273 pp., illus., glossary, biblio., index. $10.95 (cloth); $5.95 (paper). - The Passionate Amateur's Guide to Archaeology in the United States. Josleen Wilson Collier Books, New York, 1980. xv + 464 pp., illus., biblio., index. $12.95 (paper). (1982)

Discover Archaeology: An Introduction to the Tools and Techniques of Archaeological Fieldwork. George Sullivan Penguin Books, New York, 1980. ix + 273 pp., illus., glossary, biblio., index. 5.95 (paper). - The Passionate Amateur's Guide to Archaeology in the United States. Josleen Wilson Collier Books, New York, 1980. xv + 464 pp., illus., biblio., index. $12.95 (paper). - Volume 47 Issue 4
ENHANCED KIDNEY GRAFT SURVIVAL WITH RETROPLACENTAL SOURCE γ-GLOBULIN (1982)

We examined the possibility that retroplacental source gamma-globulin (RPGG), with its content of anti-HLA antibodies, would improve cadaver kidney graft survival rates. In a 5-year controlled prospective study of 208 transplants, we found that the addition of RPGG to a standard immunosuppressive drug regimen (azathioprine and prednisone) resulted in significant improvement of the cumulative survival rate (CSR) of first and second grafts. At 2 years, the overall CSR of first grafts increased from a control value of 37% +/- 6 to 52% +/- 6 (P = 0.037). Among second graft recipients, the CSR increased from a value of 19% +/- 8 to 50% +/- 10 (P = 0.014). This improvement in graft survival was seen as early as 3 months after surgery and was sustained through 3 years without added recipient morbidity or mortality. When recipient populations were stratified for various factors, those groupings remonstrative of an intact or active humoral immune response capacity were found to have the highest survival rates in the study; 2-year graft CSRs of 70% +/- 6 and 65% +/- 10 were found in recipients with preformed antibody resulting from blood transfusions (P - 0.003) and cytomegalovirus infectivity (P = 0.0006), respectively. These findings indicate that the improved graft survival seen in this study may have resulted from a recipient's immunological response to challenge with RPGG.
Research and Development of a Fluidized Bed Catalyst for Production of Phthalic Anhydride and Commercialization Thereof. (Part 1). Preparation of Silica-supported V2O5 Catalyst Having Good Attrition Resistance and Catalytic Properties. (2000)

Badger 社 (現 Raytheon Engineers & Constructors) から技術導入されたナフタレン流動層気相酸化法無水フタル酸製造触媒 (米国 Davison 社#906: 組成V2O5-K2SO4-SO3/SiO2) は触媒の耐摩耗性, 流動性および触媒寿命などに問題があった。本報ではこの導入触媒の活性劣化原因を性状面から究明し, 含浸法触媒に特有の現象 (粒子内および粒子間での活性成分の偏在) であることを明らかにした。この結果をもとに, 耐摩耗性の優れた球形の高活性, 高寿命型触媒の開発のため, 平均粒子径が5～12nmの範囲の特殊性状のシリカゾルをシリカ源とする新たな流動層触媒の製法を開発し, 活性成分組成比および物性の適正化を検討した。得られた適正組成触媒を実機プラントに適用し, 従来の導入触媒に比べ大幅な無水フタル酸収率の向上を達成した。
Response: absence of CCR5 intracellular pools in most CD4 and CD8 T cells (2011)

Guglielmi et al report that a minority (15%) of circulating CD4+ T cells show intracellular staining with a murine monoclonal antibody, 2D7, which recognizes a domain on the second extracellular loop of CCR5. They find that this staining is not seen in circulating cells obtained from a patient
Développement d'architectures de filtres à base de résonateurs à ondes acoustiques de volume et contribution à l'intégration dans une technologie avancée Silicium industrielle pour des applications radio fréquences. (2007)

Thèse d'Université de Limoges
A Short and New Synthesis of Optically Pure (+)-Retronecine (1994)

A short synthesis of (+)-retronecine from (R)-3-hydroxy pyrrolidine, utilizing [3 + 2] cycloaddition of cyclic non-stabilized azomethine ylide as the key step is reported.
Optical full Hadamard matrix multiplexing and noise effects (2009)

Hadamard multiplexing provides a considerable SNR boost over additive random noise but Poisson noise such as photon noise reduces the boost. We develop the theory for full H-matrix Hadamard transform imaging under additive and Poisson noise effects. We show that H-matrix encoding results in no effect on average on the noise level due to Poisson noise sources while preferentially reducing additive noise. We use this result to explain the wavelength-dependent varying SNR boost in a Hadamard hyperspectral imager and argue that such a preferential boost is useful when the main noise source is indeterminant or varying.
Chemical Bonding States at Copper/Graphite Interfaces with Additional Elements (2005)

Chemical bonding states of Cu/graphite interfaces were investigated by using the first-principles molecular orbital method with model clusters. It was found that Cu atoms in the interface layer undergo electronic overlap with C atoms at the first and second neighboring sites. Chemical bonding strength across the interface is strongly affected by the presence of Ti or Zr in the interface layer. The interfacial attractive interaction increases by doping the larger-sized Ti or Zr atom, although the interlayer distances between Cu and graphite become larger. Therefore, the interface strength of Cu/graphite can be improved by the additional elements at the interface.
Polarized but differential localization and recruitment of STIM1, Orai1 and TRPC channels in secretory cells (2011)

Polarized Ca(2+) signals in secretory epithelial cells are determined by compartmentalized localization of Ca(2+) signaling proteins at the apical pole. Recently the ER Ca(2+) sensor STIM1 (stromal interaction molecule 1) and the Orai channels were shown to play a critical role in store-dependent Ca(2+) influx. STIM1 also gates the transient receptor potential-canonical (TRPC) channels. Here, we asked how cell stimulation affects the localization, recruitment and function of the native proteins in polarized cells. Inhibition of Orai1, STIM1, or deletion of TRPC1 reduces Ca(2+) influx and frequency of Ca(2+) oscillations. Orai1 localization is restricted to the apical pole of the lateral membrane. Surprisingly, cell stimulation does not lead to robust clustering of native Orai1, as is observed with expressed Orai1. Unexpectedly, cell stimulation causes polarized recruitment of native STIM1 to both the apical and lateral regions, thus to regions with and without Orai1. Accordingly, STIM1 and Orai1 show only 40% colocalization. Consequently, STIM1 shows higher colocalization with the basolateral membrane marker E-cadherin than does Orai1, while Orai1 showed higher colocalization with the tight junction protein ZO1. TRPC1 is expressed in both apical and basolateral regions of the plasma membrane. Co-IP of STIM1/Orai1/IP(3) receptors (IP(3) Rs)/TRPCs is enhanced by cell stimulation and disrupted by 2-aminoethoxydiphenyl borate (2APB). The polarized localization and recruitment of these proteins results in preferred Ca(2+) entry that is initiated at the apical pole. These findings reveal that in addition to Orai1, STIM1 likely regulates other Ca(2+) permeable channels, such as the TRPCs. Both channels contribute to the frequency of [Ca(2+) ] oscillations and thus impact critical cellular functions.
Seasonal Rhinitis in a Cat Sensitized to Japanese Cedar (Cryptomeria japonica) Pollen. (2001)

A cat showing seasonal allergic symptoms of rhinitis was examined for reactivities to Japanese cedar (Cryptomeria japonica, CJ) pollen allergen by intradermal skin test (IDST), Prausnitz-Kustner (P-K) test, and lymphocyte blastogenic response. In IDST for 26 common allergens. the cat showed a positive reaction to CJ pollen allergen. P-K test using CJ pollen allergen also showed a positive reaction, indicating the presence of serum IgE specific to CJ pollen. In the lymphocyte blastogenic response, the stimulation index in the presence of CJ pollen allergen was 2.4. These data suggested that the seasonal rhinitis observed in the cat was caused by the sensitization to CJ pollen allergen.
Vibrational Spectroscopy in the Detection of Cancer (2001)

Abstract The sections in this article are Introduction Vibrational Spectroscopy – Sensitivity to Structures of Biological Molecules Nucleic Acids Proteins Lipids Carbohydrates Cells and Tissues Cell Biology Cancer Experimental Techniques Histological Samples IR and R aman Sampling Techniques Liquid Sampling Solid Sampling Macroscopic Sampling IR Transmission and Reflection Fiber‐Optic Probes R aman Spectroscopy Microscopic Sampling Mid‐Infrared Synchrotron Radiation Mid‐Infrared FPA Detection CCD Detection in R aman Spectroscopic Sample Applications Breast Cervix Conclusions Acknowledgments
Reduced specificity for the local lymph node assay for lipophilic chemicals: Implications for the validation of new approach methods for skin sensitization (2023)

Meaningful and accurate reference data are crucial for the validation of New Approach Methodologies (NAMs) in toxicology. For skin sensitization, multiple reference datasets are available including human patch test data, guinea pig data and data from the mouse local lymph node assay (LLNA). When assessed against the LLNA, a reduced sensitivity has been reported for in vitro and in chemico assays for lipophilic chemicals with a LogP ≥3.5, resulting in reliability restrictions within the h-CLAT OECD test guideline. Here we address the question of whether LLNA data are an appropriate reference for chemicals in this physicochemical range. Analysis of LLNA vs human reference data indicates that the false-discovery rate of the LLNA is significantly higher for chemicals with LogP ≥3.5. We present a mechanistic hypothesis whereby irritation caused by testing lipophilic chemicals at high test doses leads to unspecific cell proliferation. The accompanying analysis indicates that for lipophilic chemicals with negative calls in in vitro and in chemico assays, resorting to the LLNA is not necessarily a better option. These results indicate that the validation of NAMs in this particular LogP range should be based on a more holistic evaluation of the reference data and not solely upon LLNA data.
Chronic Amiodarone Reduces Transmural Dispersion of Repolarization in the Canine Heart (1997)

Our data demonstrate for the first time a direct effect of chronic amiodarone treatment to differentially alter the cellular electrophysiology of ventricular myocardium so as to produce an important decrease in transmural dispersion of repolarization, especially under conditions in which dispersion is exaggerated. These results may contribute to our understanding of the effectiveness of amiodarone in the treatment of life-threatening arrhythmias as well as to our understanding of the low incidence of proarrhythmia attending therapy with chronic amiodarone in comparison with other Class III agents.
Holistic Query Expansion Using Graphical Models. (2004)

this paper we present a method for answering relationship questions, as posed for example in the spring of 2003 evaluation exercise of the AQUAINT program, which has funded this research
Textural Properties of Beta‐lactoglobulin – Sodium Alginate Mixed Gels at Large Scale Deformation (2012)

The denaturation and gelling properties of mixed systems of β-lactoglobulin and sodium-alginate have been investigated as a function of alginate molecular weight, chemical composition, concentration, pH and ionic strength. Differential scanning calorimetry and small strain oscillatory measurements showed that denaturation temperature were lower than the gelling temperatures under the conditions examined. The denaturation temperatures were dependent on both pH and ionic strength, but unaffected by alginate concentration and type. The mechanical and textural properties of mixed gels of β-lactoglobulin and sodium alginate were dependent on several factors; the gel strength increased as a function of alginate concentration under ambient conditions, and decreased as the pH and/or the ionic strength were changed. High molecular weight alginate gave the most pronounced effects, probably due to the accessibility of the alginate for protein binding. The chemical composition of the alginate had negligible effect on the mechanical properties of the gels. PRACTICAL APPLICATIONS: As both proteins and polysaccharides are widely used in the food industry, it is important to understand the interactions between these two biopolymers in order to envisage final product properties. The present article gives an overview of several important parameters when mixing β-lactoglobulin and sodium alginate. β-Lactoglobulin is the main protein in whey and one of the major food protein ingredients. Alginate is a polysaccharide that is often used in the food industry because of its functional properties. This study shows that if the conditions and the alginate type are adequately chosen, the textural properties of food products can be controlled and tailored.
New Exposure Indicators for Digital Radiography Simplified for Radiologists and Technologists (2012)

Both the International Electrotechnical Commission (IEC standard 62494-1) and the American Association of Physicists in Medicine (AAPM Task Group 116) have developed similar standards for monitoring exposure in digital radiography to eliminate proprietary and confusing terminology. Radiologists and technologists will need to learn three new terms--exposure index, target exposure index, and deviation index--to understand the new standards.
Supplementary Figure Legends from MicroRNA-31 Predicts the Presence of Lymph Node Metastases and Survival in Patients with Lung Adenocarcinoma (2023)

&lt;p&gt;PDF file, 64K.&lt;/p&gt;
Pareto-Based Multi-Objective Optimization of Recuperated S-CO2 Brayton Cycles (2014)

In this paper, two configurations of the S-CO2 Brayton cycles (i.e., the single-recuperated and recompression cycles) are thermodynamically modeled and optimized through a multi-objective approach. Two semi-conflicting objectives, i.e., cycle efficiency (ηc) and cycle specific power (Φsp) are maximized simultaneously to achieve Pareto optimal fronts. The objective of maximum cycle efficiency is to have a smaller and less expensive solar field, and a lower fuel cost in case of a hybrid scheme. On the other hand, the objective of maximum specific power provides a smaller power block, and a lower capital cost associated with recuperators and coolers. The multi-objective optimization is carried out by means of a genetic algorithm which is a robust method for multidimensional, nonlinear system optimization. The optimization process is comprehensive, i.e., all the decision variables including the inlet temperatures and pressures of turbines and compressors, the pinch point temperature differences, and the mass flow fraction of the main compressor are optimized simultaneously. The presented Pareto optimal fronts provide two optimum trade-off curves enabling decision makers to choose their desired compromise between the objectives, and to avoid naive solution points obtained from a single-objective optimization approach. Moreover, the comparison of the Pareto optimal fronts associated with the studied configurations reveals the optimum operational region of the recompression configuration where it presents superior performance over the single-recuperated cycle.
Collision Resolution and Interference Elimination in Multiaccess\n Communication Networks (2017)

We define a multiaccess communication scheme that effectively eliminates\ninterference and resolves collisions in many-to-one and many-to-many\ncommunication scenarios. Each transmitter is uniquely identified by a steering\nvector. All signals issued from a specific transmitter will be steered into the\nsame single-dimensional or double-dimensional subspace at all receivers hearing\nthis transmission. This subspace is orthogonal to the noise subspace at a\nreceiver and the signals within the subspace can be extracted using the\nroot-MUSIC method. At high SNR, local channel knowledge and strict\nsynchronization, the algorithm asymptotically achieves full network capacity on\ncondition that a channel remains constant within a single time slot. Without\nsynchronization, the worst case asymptotic performance is still greater than\nthe $50\\%$ throughput achieved by collision resolution algorithms and\ninterference management techniques like interference alignment.\n
Ultra-fast segmentation and quantification of poly-metallic nodule coverage in high-resolution digital images (2013)

The exploration of the seafloor regarding the abundances of poly-metallic nodules has received growing attention recently. Image-based exploration using camera-equipped deep-sea observation systems has been introduced successfully to increase spatial resolution in the exploration process but the evaluation and interpretation of the huge amount of image data creates a serious bottleneck. In this paper we present a new software solution to the problem of automatic detection and segmentation of poly-metallic nodules in underwater images, which is not only accurate but so fast that real time image analysis is definitely in reach. Thus, an application on an AUV (Autonomous Underwater Vehicle) seems possible in the near future.
Drops Sitting on a Tilted Plate: Receding and Advancing Pinning (2012)

The wetting behavior of a liquid drop sitting on an inclined plane is investigated experimentally and theoretically. Using Surface Evolver, the numerical simulations are performed based on the liquid-induced defect model, in which only two thermodynamic parameters (solid-liquid interfacial tensions before and after wetting) are required. A drop with contact angle (CA) equal to θ is first placed on a horizontal plate, and then the plate is tilted. Two cases are studied: (i) θ is adjusted to the advancing CA (θ(a)) before tilting, and (ii) θ is adjusted to the receding CA (θ(r)) before tilting. In the first case, the uphill CA declines and the downhill CA remains unchanged upon inclination. When the tilted drop stays at rest, the pinning of the receding part of the contact line (receding pinning) and the depinning of the advancing part of the contact line (advancing depinning) are observed. The free energy analysis reveals that upon inclination, the reduction of the solid-liquid free energy dominates over the increment of the liquid-gas free energy associated with shape deformation. In the second case, the downhill CA grows and the uphill CA remains the same upon inclination. Advancing pinning and receding depinning are noted for the tilted drop at rest. The free energy analysis indicates that upon inclination, the decrease of the liquid-gas free energy compensates the increment of the solid-liquid free energy. The experimental results are in good agreement with those of simulations.
Mechanism of human Hb switching: a possible role of the kit receptor/miR 221-222 complex Short title: The regulation of human Hb switching (2009)

Background. The human hemoglobin switch (HbF->HbA) takes place in the peri/post-natal period. In adult life, however, the residual HbF (<1%) may be partially reactivated by chemical inducers and/or cytokines such as the kit ligand (KL). MicroRNAs (miRs) play a pivotal role in normal hematopoiesis: downmodulation of miR-221/222 stimulates human erythropoietic proliferation through upmodulation of the kit receptor. Design and Methods. We have explored the possible role of kit/KL in perinatal Hb switching by evaluating: i) the expression levels of both kit and KL on CD34+ cells and in plasma isolated from pre-, mid- and full-term cord blood (CB) samples; ii) the reactivation of HbF synthesis in KLtreated unilineage erythroid cell cultures; iii) the functional role of miR-221/222 in HbF production. Results. In perinatal life kit expression showed a gradual decline directly correlated to the decrease of HbF (from 80-90% to <30%). Moreover, in full-term CB erythroid cultures KL induced a marked increase of HbF (up to 80%), specifically abrogated by addition of the kit inhibitor Imatinib, thus reversing the Hb switch. MiR-221/222 expression exhibited rising levels during peripost-natal development. In functional studies, overexpression of these miRs in CB progenitors caused a remarkable decrease in kit expression, erythroblast proliferation and HbF content, whereas their suppression induced opposite effects. Conclusions. In conclusion, our studies indicate that human perinatal Hb switching is under control of the kit receptor/miR 221-222 complex. We do not exclude, however, that other mechanisms (i.e., glucocorticoids and the HbF inhibitor BCL11A) may also contribute to the peri/post-natal Hb switch.