gebhart/patrepeval huggingface dataset:
The SUGAR-DEPENDENT1 Lipase Limits Triacylglycerol Accumulation in Vegetative Tissues of Arabidopsis  (2013)

There has been considerable interest recently in the prospect of engineering crops to produce triacylglycerol (TAG) in their vegetative tissues as a means to achieve a step change in oil yield. Here, we show that disruption of TAG hydrolysis in the Arabidopsis (Arabidopsis thaliana) lipase mutant sugar-dependent1 (sdp1) leads to a substantial accumulation of TAG in roots and stems but comparatively much lower TAG accumulation in leaves. TAG content in sdp1 roots increases with the age of the plant and can reach more than 1% of dry weight at maturity, a 50-fold increase over the wild type. TAG accumulation in sdp1 roots requires both ACYL-COENZYME A:DIACYLGLYCEROL ACYLTRANSFERASE1 (DGAT1) and PHOSPHATIDYLCHOLINE:DIACYLGLYCEROL ACYLTRANSFERASE1 and can also be strongly stimulated by the provision of exogenous sugar. In transgenic plants constitutively coexpressing WRINKLED1 and DGAT1, sdp1 also doubles the accumulation of TAG in roots, stems, and leaves, with levels ranging from 5% to 8% of dry weight. Finally, provision of 3% (w/v) exogenous Suc can further boost root TAG content in these transgenic plants to 17% of dry weight. This level of TAG is similar to seed tissues in many plant species and establishes the efficacy of an engineering strategy to produce oil in vegetative tissues that involves simultaneous manipulation of carbohydrate supply, fatty acid synthesis, TAG synthesis, and also TAG breakdown.
Review on laser powder injection additive manufacturing of novel alloys and composites (2016)

In this paper, recent research and progress associated with development of alloys and composites using LENS are reviewed. The microstructure of novel materials can be tailored by controlling both composition and process parameters. For process control, closed-loop diagnostics and controls such as in-situ molten pool sensor and Z-height control subsystems are utilized, while the thermal behavior measurement with thermal imaging methods and numerical simulation are also investigated. The existing problems with residual stress and porosity in deposited materials are discussed.
Intestinal Virome in Patients With Alcoholic Hepatitis (2020)

In conclusion, intestinal viral taxa are altered in fecal samples from patients with AH and associated with disease severity and mortality. Our study describes an intestinal virome signature associated with AH.
Sheet Hydroforming Simulation of a License-Plate-Pocket Panel (2004)

The objective of this research was to numerically study the application of the sheet hydroforming process to a real‐world complex automotive part. In sheet hydroforming, one or both surfaces of the sheet metal are supported with a pressurized viscous fluid to assist with the forming of the part where a female die is not required. The pressurized fluid supports the blank during the forming process, delays the onset of material failure and acts as an active blank‐holding force. The commercial finite element analysis code LS‐Dyna was used to simulate the process using Barlat’s anisotropic yield function to represent material behavior during deformation. Pressure and blank holding force profiles were developed for forming this complex part without defects. In addition, it is numerically shown that through the application of an appropriate fluid pressure profile, wrinkles could be eliminated.
900. Specific AAV Serotypes Stably Transduce Hippocampal and Cortical Cultures with High Efficiency and Low Toxicity (2006)

Cultures of dissociated neurons and astrocytes are frequently used by neurobiologists to study neuronal physiology and pathophysiology. However, current methods for gene delivery into neurons in vitro are limited by very low efficiency, short-term gene expression, toxicity and/or the requirement to transfect on the day of plating. We hypothesized that adeno-associated viral (AAV) vectors might represent an efficient and less toxic method for neuronal transduction. We tested AAV transduction of primary cultures of 150,000 E18 rat cortical or hippocampal neurons and astrocytes using six serotypes (AAV1, 2, 6, 7, 8, and 9), each containing the same AAV-2-based genome encoding CMV-GFP. Mature cultures of hippocampal neurons were transduced at 4 weeks post-plating by AAV1, 2, 7, 8 and 9, resulting in GFP expression for at least 1 week. AAV2 (2.5 E+11 genome equivalents, GE) was particularly effective in transducing the mature hippocampal cultures. Expression of GFP was detectable by 1 week post-transduction in 40–60% of the cells, localized predominantly in cells expressing the neuronal marker MAP2, and stable for up to 4 weeks (the life of the culture) without inducing cell death. Immature hippocampal cultures were exposed at 1 day post-plating to AAV1, 2, 6, 7 and 9, and transduced by AAV1, 2, 7 and 9. The AAV9 serotype (5E+11GE) transduced 50–70% of cells in the d1 hippocampal cultures and GFP expression was stable for 4 weeks. The AAV6 serotype caused cell death in both the mature and immature hippocampal cultures and the AAV7 serotype transduced both neurons and astrocytes. To determine whether cortical neurons were transduced by the same vectors, AAV1, 6, 7 and 9 were applied to cortical cultures at 4 weeks post-plating. All 4 serotypes transduced cortical cells and the GFP expression was stable for 1–2 weeks. In both hippocampal and cortical cultures, cell death occurred at later time points (2–4 weeks) after transduction although toxicity was not related to the volume of vector applied or GFP expression. Work is ongoing to quantify the percentages of neurons and astrocytes transduced by each serotype and to explore the in vitro transduction patterns of AAV vectors with engineered capsid proteins. These results should make possible a wide range of neurobiological experiments in cultured neurons, including delivery of shRNAs. Supported by NS040978 (DW) and P30-DK-047757.
High-resolution stimulated Brillouin gain spectroscopy in glasses and crystals (1993)

Theory and measurements are presented for stimulated Brillouin gain spectroscopy in anisotropic media. The coupling between arbitrary pump and Stokes waves and the corresponding acoustic wave is formulated in terms of the elastic displacement vector and the photoelastic tensor. A propagation equation that fully includes transient effects is obtained for the Stokes wave. In the limit of small-signal gains this propagation equation can be solved analytically, yielding expressions that relate experimentally accessible quantities to material properties. Absolute Brillouin steady-state gain coefficients, linewidths, and frequency shifts are thereby determined in a number of optical materials at 532 nm. The Brillouin gain coefficient for fused silica is measured by three techniques, providing the reference for absolute gain measurements. Quantitative agreement is found between theory and experiment for transient effects on stimulated Brillouin scattering.
Information Fusion for Action Recognition with Deeply Optimised Hough Transform Paradigm (2016)

International audience
Activated transglutaminase from <i>Streptomyces mobaraensis</i> is processed by a tripeptidyl aminopeptidase in the final step (2003)

Transglutaminase (TGase) from Streptomyces mobaraensis is secreted as a precursor protein which is completely activated by the endoprotease TAMEP, a member of the M4 protease family [Zotzel, J., Keller, P. & Fuchsbauer, H.-L. (2003) Eur. J. Biochem. 270, 3214-3222]. In contrast with the mature enzyme, TAMEP-activated TGase exhibits an additional N-terminal tetrapeptide (Phe-Arg-Ala-Pro) suggesting truncation, at least, by a second protease. We have now isolated from the culture broth of submerged colonies a tripeptidyl aminopeptidase (SM-TAP) that is able to remove the remaining tetrapeptide. The 53-kDa peptidase was purified by ion-exchange and phenyl-Sepharose chromatography and subsequently characterized. Its proteolytic activity was highest against chromophoric tripeptides at pH 7 in the presence of 2 mm CaCl2. EDTA and EGTA (10 mm) both diminished the proteolytic activity by half. Complete inhibition was only achieved with 1 mm phenylmethanesulfonyl fluoride, suggesting that SM-TAP is a serine protease. Alignment of the N-terminal sequence confirmed its close relation to the Streptomyces TAPs. That removal of Phe-Arg-Ala-Pro from TAMEP-activated TGase by SM-TAP occurs in a single step was confirmed by experiments using various TGase fragments and synthetic peptides. SM-TAP was also capable of generating the mature N-terminus by cleavage of RAP-TGase. However, AP-TGase remained unchanged. As SM-TAP activity against chromophoric amino acids such as Pro-pNA or Phe-pNA could not be detected, the tetrapeptide of TAMEP-activated TGase must be removed without formation of an intermediate.
A 60 GHz Transceiver for Low Power Ad Hoc Networks (2012)

This paper presents a low power consumption architecture of RF transceiver using impulse radio signals operating at 60 GHz. Both intrinsic features and related technologies of this operating frequency are detailed and the specific topology of this communication and geo-localization system is introduced. The performance of this low power consumption system is validated experimentally. The emitter exhibits an energy efficiency down to 10 pJ/bit and data rates up to 10 Mbps. Geo-localisation ability of this 60 GHz system is also experimentally demonstrated with localisation accuracy in the range of 40 cm.
Effective Carrier Mobility Extraction Based on RF Modeling for Highly Leaky MOSFET Devices with Short Channel Length and Small Area (2009)

In this paper, a novel method for effective mobility extraction of the advanced MOSFET devices using RF modeling scheme is proposed. The proposed method is robust to high gate leakage current and parasitic source/drain resistance. Also, this method can substantially reduce error from drain bias mismatch between channel conductance and gate-to-channel capacitance measurement, uses only single device, and is applicable to small-area devices. nMOSFET with HfSiON gate dielectric and TiN gate electrode is demonstrated with the proposed method.
Virtual reality in posturography (2006)

Balance dysfunctions are common, especially among elderly people. Present methods for the diagnosis and evaluation of severity of dysfuntion have limited value. We present a system that makes it easy to implement different visual and mechanical perturbations for clinical investigations of balance and visual-vestibular interaction. The system combines virtual reality visual stimulation with force platform posturography on a moving platform. We evaluate our contruction's utility in a classification task between 33 healthy controls and 77 patients with Ménière's disease, using a series of tests with different visual and mechanical stimuli. Responses of patients and controls differ significantly in parameters computed from stabilograms. We also show that the series of tests achieves a classification accuracy slightly over 80% between controls and patients.
Supplementary Table S1 from Atypical KRAS&lt;sup&gt;G12R&lt;/sup&gt; Mutant Is Impaired in PI3K Signaling and Macropinocytosis in Pancreatic Cancer (2023)

&lt;p&gt;Structure factors and crystal information&lt;/p&gt;
Immunotherapy for Ovarian Cancer: What's Next? (2010)

In the past decade, we have witnessed important gains in the treatment of ovarian cancer; however, additional advances are required to reduce mortality. With compelling evidence that ovarian cancers are immunogenic tumors, immunotherapy should be further pursued and optimized. The dramatic advances in laboratory and clinical procedures in cellular immunotherapy, along with the development of powerful immunomodulatory antibodies, create new opportunities in ovarian cancer therapeutics. Herein, we review current progress and future prospects in vaccine and adoptive T-cell therapy development as well as immunomodulatory therapy tools available for immediate clinical testing.
Allergic Fungal Sinusitis-Induced Visual Loss (1999)

In this report we review 56 adult and 26 pediatric patients who presented to our practice with pathologically confirmed allergic fungal sinusitis from 1989 to 1997. Of this group, three patients presented with visual loss and were treated with prompt surgical decompression followed by immunomodulation.
Cerebral Autoregulation in Hypertension and Ischemic Stroke: A Mini Review (2017)

Aging and chronic hypertension are associated with dysfunction in vascular smooth muscle, endothelial cells, and neurovascular coupling. These dysfunctions induce impaired myogenic response and cerebral autoregulation, which diminish the protection of cerebral arterioles to the cerebral microcirculation from elevated pressure in hypertension. Chronic hypertension promotes cerebral focal ischemia in response to reductions in blood pressure that are often seen in sedentary elderly patients on antihypertensive therapy. Cerebral autoregulatory dysfunction evokes Blood-Brain Barrier (BBB) leakage, allowing the circulating inflammatory factors to infiltrate the brain to activate glia. The impaired cerebral autoregulation-induced inflammatory and ischemic injury could cause neuronal cell death and synaptic dysfunction which promote cognitive deficits. In this brief review, we summarize the pathogenesis and signaling mechanisms of cerebral autoregulation in hypertension and ischemic stroke-induced cognitive deficits, and discuss our new targets including 20-Hydroxyeicosatetraenoic acid (20-HETE), Gamma-Adducin (Add3) and Matrix Metalloproteinase-9 (MMP-9) that may contribute to the altered cerebral vascular function.
Fluidization Behavior in a Gas- Solid Fluidized Bed with Thermally Induced Inter-Particle Forces (2011)

In this work, a new approach for increasing and controlling inter-particle forces (IPFs) was applied. This method used a spherical inert particle coated with a polymer material having a low glass transition temperature. Since IPFs depend on the temperature of the coated particles, they can be easily controlled by the temperature of the inlet air. For this reason, the temperature of the system was varied uniformly near the glass transition temperature of the polymer, between 20 – 40C, to investigate the effect of IPFs on fluidization behavior at low and high gas velocities.
Small-Signal and Signal-Flow-Graph Modeling of Switched Z-Source Impedance Network (2005)

The Z-source inverter is a recently proposed converter topology that uses a unique X-shaped impedance network on its dc side for achieving both voltage-buck and boost capabilities. In the process of designing control schemes for the Z-source inverter, knowledge of the transfer function representing its unique dc impedance network is essential. Toward this end, this letter presents dynamic small-signal modeling of the Z-source impedance network using perturbed mathematical analysis and a signal-flow graph with parasitic components taken into consideration. In particular, the developed average control-to-output model reveals the presence of a right-hand-plane zero in the network transfer function, whose trajectories with variations in network parameters can be studied using classical root-locus analyses. Using the graphical signal-flow modeling approach, various disturbance-to-output transfer functions can also be derived with their parameter sensitivity similarly studied. Lastly, simulation and experimental results are presented for verifying the dynamic phenomena identified in this letter.
Effect of Inorganic Zn-primer Coating on Corrosion Resistance of Fe-Cr and Fe-Cr-Al Alloys in High Chloride Environments (2015)

It is well known that the corrosion resistance of steels is improved by the addition of alloying elements. For stainless steels, a large amount of Cr is added to obtain good corrosion resistance and the addition of rare metals such as Mo and Ni etc. are applied for further improvement of the corrosion resistance depending on corrosion environments. On the other hand, we investigated the effects of combining the addition of alloying elements and surface treatment aiming to develop new corrosion resistant steel at lower cost by reducing the content of alloying elements. We found that 6% Cr contained steel with inorganic zinc primer showed excellent anti-rust resistance in high chloride environments. Besides, the apparent rust area and maximum corrosion depth of 6% Cr contained steel were largely reduced by the addition of Al.
SFC (2012)

To support reprogramming in embedded network systems (ENSs), we present SFC, a simple flow control protocol for communication between serial devices, which enables fast and reliable data transmission. The reprogramming strategy uses a boot loader, responsible for receiving the application program over a serial communication channel, storing it in the program memory space, and then initiating execution. SFC is used as the communication protocol for the boot loader. SFC uses a finite state automata to control the send and receive mechanisms, while introducing minimal protocol overhead. The automata utilizes synchronization and acknowledgment mechanisms to achieve high reliability. We first describe the boot loader design and the reprogramming strategy. We then describe the design and implementation of the SFC protocol and present experimental results to demonstrate its performance and efficacy.
A comparison of surface-modeling techniques (2002)

Solution techniques based on surface integral equations (SIEs) are widely used in computational electromagnetics. Formulations employing SIEs express the unknown function on the defining surface of the problem geometry. Thus, both the surface and the unknown function defined on it have to be accurately represented in the solution algorithm. However, real-life problems usually involve arbitrary geometries with curved surfaces, which require either exact or higher-order geometry models. The dependence of the accuracy of the solution on the geometry modeling is investigated. The use of different basis functions to represent the unknown functions on curved surfaces is also mentioned and the problem of plane-wave scattering by a perfect electric conductor sphere is considered.
RNA-seq analysis of the salivary glands and midgut of the Argasid tick Ornithodoros rostratus (2019)

Ornithodoros rostratus is a South American argasid tick which importance relies on its itchy bite and potential as disease vector. They feed on a wide variety of hosts and secrete different molecules in their saliva and intestinal content that counteract host defences and help to accommodate and metabolize the relatively large quantity of blood upon feeding. The present work describes the transcriptome profile of salivary gland (SG) and midgut (MG) of O. rostratus using Illumina sequencing. A total of 8,031 contigs were assembled and assigned to different functional classes. Secreted proteins were the most abundant in the SG and accounted for ~67% of all expressed transcripts with contigs with identity to lipocalins and acid tail proteins being the most representative. On the other hand, immunity genes were upregulated in MG with a predominance of defensins and lysozymes. Only 10 transcripts in SG and 8 in MG represented ~30% of all RNA expressed in each tissue and one single contig (the acid tail protein ORN-9707) represented ~7% of all expressed contigs in SG. Results highlight the functional difference of each organ and identified the most expressed classes and contigs of O. rostratus SG and MG.
Power-Aware Virtual Network Function Placement and Routing using an Abstraction Technique (2018)

The Network Function Virtualization (NFV) is very promising for efficient provisioning of network services and is attracting a lot of attention. NFV can be implemented in commercial off-the-shelf servers or Physical Machines (PMs), and many network services can be offered as a sequence of Virtual Network Functions (VNFs), known as VNF chains. Furthermore, many existing network devices (e.g., switches) and collocated PMs are underutilized or over-provisioned, resulting in low power-efficiency. In order to achieve more energy efficient systems, this work aims at designing the placement of VNFs such that the total power consumption in network nodes and PMs is minimized, while meeting the delay and capacity requirements of the foreseen demands. Based on existing switch and PM power models, we propose a Integer Linear Programming (ILP) formulation to find the optimal solution. We also propose a heuristic based on the concept of Blocking Islands (BI), and a baseline heuristic based on the Betweenness Centrality (BC) property of the graph. Both heuristics and the ILP solutions have been compared in terms of total power consumption, delay, demands acceptance rate, and computation time. Our simulation results suggest that BI-based heuristic is superior compared with the BC-based heuristic, and very close to the optimal solution obtained from the ILP in terms of total power consumption and demands acceptance rate. Compared to the ILP, the proposed BI-based heuristic is significantly faster and results in 22% lower end-to-end delay, with a penalty of consuming 6% more power in average.
Surface‐Mediated Alignment of Long Pitch Chiral Nematic Liquid Crystal Structures (2018)

Abstract Chiral nematic liquid crystals are stimuli‐responsive soft materials that can self‐organize into complex chiral superstructures. Control and manipulation of the chiral ordering is essential in the development of functional electro‐optic devices. Here, the effect of surface topography is combined with an applied electric field to steer the directional growth of cholesteric fingers in a cell with thickness larger than the chiral pitch. The periodic e‐beam resist grating gives rise to micrometer‐scale periodic variations of the surface anchoring. By applying a voltage to the cell, controlled growth of cholesteric fingers along the surface grating is observed. The 2D liquid crystal director configuration at different voltages is identified by numerical simulations based on finite element Q‐tensor theory. The results demonstrate that samples with modulated surface chemistry lead to enhanced control over the formation of chiral structures. This can stimulate the development of electro‐optic devices (diffraction gratings, lasers, displays, etc.) with improved characteristics.
Intramolecular O–H ⋯ O–Ni and N–H ⋯ O–Ni hydrogen bonding in nickel diphenylphosphinoenolate phenyl complexes: role in catalytic ethene oligomerisation; crystal structure of [NiPH{Ph<sub>2</sub>PCHC(O)(o-C<sub>6</sub>H<sub>4</sub>NHPh)}(PPh<sub>3</sub>)] (1994)

Nickel diphenylphosphinoenolate complexes are prepared from ortho-HX-substituted phenacylidenetriphenylphosphoranes (X = O, NMe, NPh) which display strong intramolecular hydrogen bonding between the enolate oxygen and the H–X function; this feature dramatically influences the molecular mass distribution of the oligomers obtained by catalytic oligomerisation of ethene.