gebhart/patrepeval huggingface dataset:
Sustained high-titer antibody responses induced by conjugating a malarial vaccine candidate to outer-membrane protein complex (2006)

The development of protein subunit vaccines to combat some of the world's deadliest pathogens such as a malaria parasite, Plasmodium falciparum, is stalled, due in part to the inability to induce and sustain high-titer antibody responses. Here, we show the induction of persistent, high-titer antibody responses to recombinant Pfs25H, a human malarial transmission-blocking protein vaccine candidate, after chemical conjugation to the outer-membrane protein complex (OMPC) of Neisseria meningitidis serogroup B and adsorption to aluminum hydroxyphosphate. In mice, the Pfs25H-OMPC conjugate vaccine was >1,000 times more potent in generating anti-Pfs25H ELISA reactivity than a similar 0.5-microg dose of Pfs25H alone in Montanide ISA720, a water-in-oil adjuvant. The immune enhancement requires covalent conjugation between Pfs25H and the OMPC, given that physically mixed Pfs25H and OMPC on aluminum hydroxyphosphate failed to induce greater activity than the nonconjugated Pfs25H on aluminum hydroxyphosphate. The conjugate vaccine Pfs25H-OMPC also was highly immunogenic in rabbits and rhesus monkeys. In rhesus monkeys, the antibody responses were sustained over 18 months, at which time another vaccination with nonconjugated Pfs25H induced strong anamnestic responses. The vaccine-induced anti-Pfs25-specific antibodies in all animal species blocked the transmission of parasites to mosquitoes. Protein antigen conjugation to OMPC or other protein carrier may have general application to a spectrum of protein subunit vaccines to increase immunogenicity without the need for potentially reactogenic adjuvants.
A Closely Coupled and Scalable High-Power Modular Inductive Charging System for Vehicles (2020)

For a high-power inductive charging solution that can be used on all types of vehicles, careful consideration must be made in all steps of the design process, including system architecture, coil geometry, and communications. Extensive studies on the effects of human exposure to electromagnetic fields (EMF) have identified recommended guidelines for electromagnetic limits in regions where humans or other living objects may frequent. Commercial wireless low power chargers today operating at 85 kHz and up to 11 kW are capable of meeting international EMF standards such as IEEE C95.1-2019. In this article, we address high-power wireless chargers designed for electrified light and heavy-duty vehicles such as buses and trucks that accept charging in hundreds of kilowatts. Laboratory studies of a modular 50–75-kW coupler confirm that leakage fields from one such module will meet U.S. and International EMF limits at the vehicle perimeter and beyond. In particular, a cluster of four such couplers operating at 65 kW each will not exceed <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$27~\mu \text{T}$ </tex-math></inline-formula> adjacent to the vehicle, decaying at 1/ <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\text{r}^{3}$ </tex-math></inline-formula> or faster. The experimental results confirm simulation and show that fringing fields meet published standards in the vehicle cabin and beyond its perimeter when operating at 260 kW.
Isobutanol production by combined in vivo and in vitro metabolic engineering (2022)

The production of the biofuel, isobutanol, in <i>E. coli</i> faces limitations due to alcohol toxicity, product inhibition, product recovery, and long-term industrial feasibility. Here we demonstrate an approach of combining both <i>in vivo</i> with <i>in vitro</i> metabolic engineering to produce isobutanol. The <i>in vivo</i> production of α-ketoisovalerate (KIV) was conducted through CRISPR mediated integration of the KIV pathway in bicistronic design (BCD) in <i>E. coli</i> and inhibition of competitive valine pathway using CRISPRi technology. The subsequent <i>in vitro</i> conversion to isobutanol was carried out with engineered enzymes for 2-ketoacid decarboxylase (KIVD) and alcohol dehydrogenase (ADH). For the <i>in vivo</i> production of KIV and subsequent <i>in vitro</i> production of isobutanol, this two-step serial approach resulted in yields of 56% and 93%, productivities of 0.62 and 0.074 g L<sup>-1</sup> h<sup>-1</sup>, and titers of 5.6 and 1.78 g L<sup>-1</sup>, respectively. Thus, this combined biosynthetic system can be used as a modular approach for producing important metabolites, like isobutanol, without the limitations associated with <i>in vivo</i> production using a consolidated bioprocess.
Calculation of modal discrimination in leaky-mode diode-laser arrays using a triangular-mesh finite-difference method (1990)

A new type of diode-laser array that emits a near-diffraction-limited beam at drive currents up to 10 times threshold has recently been reported. 1 These devices, denoted leaky-mode arrays because of their tendency to lase on radiation modes, show great potential as high-power sources of focusable radiation. Realization of this potential, however, necessitates accurate modeling, which has so far proved difficult because of several problems. First, the use of the effective index method has been shown to be inaccurate for these arrays so that full two-dimensional (2D) waveguide modeling is necessary. 2 Second, the use of highly absorbing GaAs regions requires a fully complex calculation. Finally, the accurate simulation of radiation modes necessitates lateral boundary conditions that are totally absorbing; i.e., they reflect no radiation back into the problem region.
Diabetic Retinopathy: Past, Present and Future (2014)

Diabetes and diabetic retinopathy cause major morbidity in the human population. Diabetes was described as long ago as 1552 B. C. in the Papyrus Ebers, and it affects many organ systems of the body, including the eye where it leads to vascular leakage and haemorrhage and results in blurred vision and ultimately total vision loss from proliferative retinopathy. Diabetic retinopathy was reported for perhaps the first time by Appolinaire Bouchardat in 1846. Three major stages of understanding this disease have occurred since then. The first phase began with the first description in 1846 and continued into the middle of the 20th century when various clinical aspects of the conditions were described. The second stage began in the 1970s with a series of interventional treatments that began with pan-retinal photocoagulation, developed through the National Eye Institute (NEI) Diabetic Retinopathy Study. This remains the mainstay of medical treatment for the ocular component of advanced diabetes. We have now entered a third phase, which portends the future based on biologic intervention at the molecular level. This work has been pioneered through the Diabetic Retinopathy Clinical Research Network (DRCR.net), established in 2002, which recently demonstrated that intraocular anti-vascular endothelial growth factor (VEGF) treatment was effective for diabetic macular edema. Success of this molecular approach will gather speed in the years ahead as we begin to understand the pathology at a cellular and system biology level.
Battery discharge aware energy feasibility analysis (2006)

It is observed that pulsed discharge currents allow to drain the battery with a higher specific power. Thus they improve the batteries durability and discharge performance. The question is how can the allowed discharge of a battery be modeled. Embedded real-time systems often rely on batteries. For these systems it is necessary to prove both, real-time feasibility and the constraint to not exceed the allowed discharge currents. This paper presents a unified approach for both objectives using the flexible model of event streams, because it allows both to model a complex allowed-discharge curve and the real-time requirements for complex task stimuli. We present the model and the calculation of the allowed and requested discharge curves. Together with the known event-stream based real-time feasibility analysis this allows a unified analysis of both aspects of the system. This work enables the modeling of complex discharge requirements of batteries for real-time systems.
Long-Term Follow-up of Rituximab and Infusional Cyclophosphamide, Doxorubicin, and Etoposide (cde) in Combination with HAART in HIVRelated Non-Hodgkin’s Lymphomas (NHL). (2008)

Abstract Background: The combination of Rituximab plus chemotherapy (CT) is more effective than CT alone in the treatment of high grade NHL. Objective: To report the long-term follow-up of CDE plus Rituximab in HIV-NHL. Methods: In June 1998, we started a phase II study using infusional CDE (Cyclophosphamide 187.5 mg/m2/day, Doxorubicin 12.5 mg/m2/day and Etoposide 60 mg/m2/day) administered by continuous intravenous infusion for 4 days every 4 weeks and Rituximab 375 mg/m2 i.v. on day 1. HAART was given concomitantly with CT. Results: Seventy-four patients (pts) have been enrolled. The median CD4+ cell count was 161 (range 3–691) and the median Performance Status was 1 (range 0–3). Diffuse large B-cell NHL was diagnosed in 72% of pts and Burkitt in 28%. Seventy per cent of pts had advanced stage (III–IV) disease and 57% of pts had an age-adjusted international prognostic index ³2. Fifty-two out of 74 pts (70%) achieved a complete remission (cr), 4/74 (5%) had a partial remission and 18 pts progressed. With a median follow-up of 61 months, only 17% of CRs have relapsed and 41/74 pts are alive. The overall survival, disease free survival and time to treatment failure (TTF) at 5 years were 56%, 81% and 52%, respectively. Only one secondary tumor (acute leukemia) has been observed. No case of late pulmonary or cardiac toxicity has been reported. Conclusions: The combination of Rituximab and CDE in HIV-NHL treated concomitantly with HAART is very active. CR rate (70%) and TTF at 5 years (52%) are comparable to those observed in high grade NHL of the general population. Our data confirm that in HAART era a high proportion of HIV-NHL can be cured. This study was supported by ISS grants.
High lipoprotein(a) is associated with major adverse limb events after femoral artery endarterectomy (2021)

Plasma Lp(a) is independently associated with first and consecutive MALE after iliofemoral endarterectomy. Hence, in patients who undergo iliofemoral endarterectomy, Lp(a) could be considered as a biomarker to enhance risk stratification for future MALE.
Risk Factors for Orthostatic Hypotension: Differences Between Elderly Men and Women (2018)

Although the prevalence of OH is similar in both sexes, there are different risk factors associated by sex. Systolic BP ≥140 mm Hg was associated with increased risk of OH only with women while BMI was a protective factor for OH in men and women.
Topography-dependent gene expression and function of common cell archetypes in large and small porcine airways (2021)

Summary The small airways of humans are affected early in several lung diseases. However, because they are relatively inaccessible, little is known about the epithelial cells that line these airways. We performed a single cell RNA-seq census of small and large airways of wild-type pigs and pigs with disrupted cystic fibrosis transmembrane conductance regulator ( CFTR ) gene. The sequencing data showed that small airway epithelia had similar major cell types as large airways but no ionocytes; moreover, lack of CFTR expression had minimal effect on the transcriptome. Small airway epithelial cells expressed a different transcriptome than large airway cells. Quantitative immunohistochemistry showed that small airway basal cells participate in epithelial barrier function. Finally, sequencing data and in vitro electrophysiologic studies suggest that small airway epithelia have a water and ion transport advantage. Our data highlight the archetypal nature of basal, secretory, and ciliated airway cells with location - dependent gene expression and function.
Preface (2003)

This double issue of the IBM Journal of Research and Development focuses on the communication technologies in IBM that are contributing to the increasingly rapid and efficient movement of data. The issue contains fourteen papers on relevant work on semiconductor processes, models, and innovative architectures, as well as circuit design and testing efforts. Its cover is a composition of various aspects of that work. A broad array of important applications are covered in the fields of wired and wireless communications, including switches, network processors, wireless LANs (local area networks), cellular radios, and optical data transceivers.
Initial Clinical Cases of the Use of a NF-.KAPPA.B Decoy at the Site of Coronary Stenting for the Prevention of Restenosis (2004)

These results indicate the clinical usefulness and safety of NF-kappaB decoy transfection after PCI, but further evaluations are necessary to confirm its clinical effectiveness.
Total synthesis of methyl (+)-pseudomonate C from carbohydrates (1983)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTotal synthesis of methyl (+)-pseudomonate C from carbohydratesJean Marie Beau, Shimpei Aburaki, Jean Rene Pougny, and Pierre SinayCite this: J. Am. Chem. Soc. 1983, 105, 3, 621–622Publication Date (Print):February 1, 1983Publication History Published online1 May 2002Published inissue 1 February 1983https://pubs.acs.org/doi/10.1021/ja00341a052https://doi.org/10.1021/ja00341a052research-articleACS PublicationsRequest reuse permissionsArticle Views379Altmetric-Citations32LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
New asthma biomarkers: lessons from murine models of acute and chronic asthma (2008)

Many patients suffering from asthma are not fully controlled by currently available treatments, and some of them display an airway remodeling leading to exaggerated lung function decline. The aim of the present study was to unveil new mediators in asthma to better understand pathophysiology and propose or validate new potential therapeutic targets. A mouse model of asthma mimicking acute or chronic asthma disease was used to select genes undergoing a modulation in both acute and chronic conditions. Mice were exposed to ovalbumin or PBS for 1, 5, and 10 wk [short-, intermediate-, and long-term model (ST, IT, and LT)], and gene expression in the lung was studied using an Affymetrix 430 2.0 genome-wide microarray and further confirmed by RT-PCR and immunohistochemistry for selected targets. We report that 598, 1,406, and 117 genes were upregulated and 490, 153, 321 downregulated at ST, IT, and LT, respectively. Genes related to mucous secretion displayed a progressively amplified expression during the allergen exposure protocol, whereas genes corresponding to growth and differentiation factors, matrix metalloproteinases, and collagens were mainly upregulated at IT. By contrast, genes related to cell division were upregulated at ST and IT and were downregulated at LT. In this study, besides confirming that Arg1, Slc26a4, Ear11, and Mmp12 genes are highly modulated throughout the asthma pathology, we show for the first time that Agr2, Scin, and Cd209e genes are overexpressed throughout the allergen exposure and might therefore be considered as suitable new potential targets for the treatment of asthma.
Crystal structure of 1,2,3,4,5-pentabromocyclopentane, C5H5Br5 (2005)

a = 10.6353(9) k,b= 8.4605(7) A, c = 11.203(1) A, = 100.799(4), V = 990.2 A*, = 4, R#(F) = 0.047, wRnffF 2 ) = 0.120, T= 150 K.
A 60 MHz 240 mW MPEG-4 video-phone LSI with 16 Mb embedded DRAM (2002)

A 240 mW single-chip MPEG-4 video-phone LSI with a 16 Mb embedded DRAM is fabricated in a 0.25 /spl mu/m CMOS, triple-well, quad-metal technology. The chip integrates a 16 Mb DRAM and three dedicated 16 b RISC processors with dedicated hardware accelerators that serve as an MPEG-4 video codec, a speech codec, and a multiplexer. It also integrates camera, display, and audio interfaces required for a video-phone system. It consumes 240 mW at 60 MHz operation, which is only 22% of the power dissipation of a conventional design. A variable threshold voltage CMOS (VTCMOS) technology is employed to reduce standby leakage current to 26 /spl mu/A, which is only 17% of the conventional CMOS design.
Artificial Antigen-Presenting Cells Permit Selective In Vitro Generation of CMV-Specific T-Cells of Desired HLA Allelic Restriction for Adoptive Immunotherapy in Recipients of HLA Disparate Allografts. (2005)

Abstract Cytomegalovirus (CMV) infections remain a major cause of morbidity and mortality following allogeneic hematopoietic cell transplantation. For recipients of HLA haplotype disparate grafts, the risk of CMV infection is especially high and extends late into the post-transplant period. Early clinical trials indicate that adoptive transfer of ex vivo generated CMV-specific T-cells from the donor can be effective in the treatment and prevention of such infections. However, because seropositive donor T-cells sensitized with autologous infected or antigen loaded APCs regularly exhibit a repertoire of CMV-specific T-cells restricted by one or two immunodominant HLA alleles, such T-cells will only be effective if these immunodominant alleles are shared by the infected host. For example, among donors inheriting HLA-B*0702, CD8+ T-cell responses to CMV-pp65 peptides are usually, and often exclusively, restricted by this allele. To address this limitation, we tested whether artificial antigen-presenting cells, consisting of murine 3T3 cells transduced to express human B7.1, LFA1, ICAM1, β 2M and HLA-A*0201 alpha chain (Papanicolaou et al., Blood 2003), could be used to generate CMV-specific T-cells restricted by this allele in a series of normal donors inheriting HLA-A*A0201 and HLA-B*0702. Accordingly, T-cells were sensitized in vitro with either autologous monocyte-derived DCs or AAPCs expressing HLA-A*A0201, each loaded with a pool of 138 overlapping pentadecapeptides spanning the CMV-pp65 protein. Thereafter, specificity of responding T-cells was identified by mapping epitopes using an intersecting matrix of peptide subpools and measuring T-cells producing IFN-γ following secondary restimulation. HLA restriction was identified by analysis of T-cell cytotoxic responses against panels of EBV BLCL sharing single HLA alleles with the donor and loaded with the targeted peptide. Findings were confirmed by quantitation of T-cell binding tetramers containing targeted epitopes bound to either HLA-A*0201 or B*0702. In each of the donors, sensitization with DCs loaded with the peptide pool resulted in the generation of CMV-pp65-specific T-cells specific for epitopes predominantly or exclusively presented by HLA-B*0702. In contrast, sensitization with peptide pool loaded AAPC led to the generation of high numbers of T-cells recognizing separate CMV-pp65 epitopes in the context of HLA-A*A0201. These HLA-A*0201-restricted T-cells were also able to lyse peptide-loaded BLCLs or PBMCs expressing HLA-A*0201. Taken together, these studies demonstrate that AAPCs expressing single HLA alleles can be used to generate fully functional T-cells specific for epitopes presented by subdominant HLA alleles. This strategy may thus permit generation of virus-specific T-cells of desired HLA-restriction for adoptive immunotherapy in HLA-disparate transplant recipients.
Instrumented Pneumatic-Impact Tester (1993)

Pneumatic-impact tester is small pressure chamber equipped with specimen holder and optical port. Device designed to test susceptibility of polymeric material to ignition by pneumatic impact in high-pressure gaseous oxygen. Used to determine differences among susceptibilities to ignition of different materials and of different batches of nominally same material proposed for use in systems containing pressurized oxygen. Also used to show characteristics of ignition and combustion.
Detection and Diagnosis of Plant‐Wide Oscillations (2007)

Abstract This paper presents some emerging techniques for detection and root‐cause diagnosis of plant‐wide oscillations, and demonstrates their efficacy through a successful industrial case study. The recently proposed autocorrelation function based method (Thornhill et al., J. Proc. Control 13, 91–100, 2003a) is used for detection of oscillations in the process measurements. Signals having common oscillations are analyzed for the presence of valve stiction using higher order statistical methods (Choudhury et al., Automatica 40, 1719–1728, 2004b) . A method employing changes in controller gain is proposed for distinguishing an internally generated oscillation from an external oscillatory disturbance. This method of changing controller gain is used to confirm the presence of control valve stiction. The proposed techniques have been used successfully to identify the root cause of plant‐wide oscillations in an industrial case study using routine operating data.