gebhart/patrepeval huggingface dataset:
Innate immune sensor LGP2 is cleaved by the Leader protease of foot-and-mouth disease virus (2018)

The RNA helicase LGP2 (Laboratory of Genetics and Physiology 2) is a non-signaling member of the retinoic acid-inducible gene-I (RIG-I)-like receptors (RLRs), whose pivotal role on innate immune responses against RNA viruses is being increasingly uncovered. LGP2 is known to work in synergy with melanoma differentiation-associated gene 5 (MDA5) to promote the antiviral response induced by picornavirus infection. Here, we describe the activity of the foot-and-mouth disease virus (FMDV) Leader protease (Lpro) targeting LGP2 for cleavage. When LGP2 and Lpro were co-expressed, cleavage products were observed in an Lpro dose-dependent manner while co-expression with a catalytically inactive Lpro mutant had no effect on LGP2 levels or pattern. We further show that Lpro localizes and immunoprecipitates with LGP2 in transfected cells supporting their interaction within the cytoplasm. Evidence of LGP2 proteolysis was also detected during FMDV infection. Moreover, the inhibitory effect of LGP2 overexpression on FMDV growth observed was reverted when Lpro was co-expressed, concomitant with lower levels of IFN-β mRNA and antiviral activity in those cells. The Lpro target site in LGP2 was identified as an RGRAR sequence in a conserved helicase motif whose replacement to EGEAE abrogated LGP2 cleavage by Lpro. Taken together, these data suggest that LGP2 cleavage by the Leader protease of aphthoviruses may represent a novel antagonistic mechanism for immune evasion.
The development of the foreign language teacher’s creative abilities in the conditions of continuous vocational education (2020)

The phenomenon of creativity is seen by different scientists as an activity, as an aspect of personal development, as a form of intellectual activity, as a synthesis of a personality’s individual psychological peculiarities and new quality conditions. The characteristics of creativity include novelty, high quality of the product, freedom of thought, originality and anomaly (illogicalness). In our research, we will view creativity as an aspect of personal development. In the most general way, creative abilities in the modern psychological and pedagogical sciences are identical to the notion of creativity, which is seen as a general, diagnostically verified capacity for creativity. The most accepted definition of this phenomenon is an individual’s ability to generate extraordinary ideas, find original solutions, and go off the bitten track. The pedagogue’s professional creativity is the ability to generate many diverse original ideas in non-prescribed circumstances of the pedagogue’s activity. It is a dynamic component of the pedagogue’s professionally significant qualities which includes the intellectual, individual, social and emotional components, whose composite development influences the effectiveness of the pedagogue’s performance and its development. This is the pedagogue’s professional creativity which is that very form of realized creative abilities which directly influences the educational process and which represents the necessary level of their development for an effective realization of modern educational technologies.
Temperature effects on trench-gate IGBTs (2002)

The switching characteristics (turn-on and turn-off) and forward conduction drop of trench-gate IGBTs are examined over a temperature range of -150 to 150/spl deg/C. An analytical description of the forward conduction voltage drop is presented based on temperature dependencies of the appropriate physical parameters and mechanisms. A physics-based PSpice model, incorporating much of the device behavior, is also described. Results from the model are compared to experimental waveforms and discrepancies are discussed.
Recovery of 2,4‐dichlorophenol from acidic aqueous streams by Membrane Aromatic Recovery System (MARS) (2004)

Abstract This study describes the successful recovery of 2,4‐dichlorophenol (DCP) from wastewater using the Membrane Aromatic Recovery System (MARS). In the MARS process a non‐porous membrane separates a wastewater stream and a stripping solution. DCP is extracted from the wastewater and concentrated in its ionic form in the stripping solution, with pH ≫ p K a DCP. The MARS extraction stage was operated in batch mode with the stripping solution placed inside, and the wastewater stream outside, the membrane tubes. Advantages of this configuration are avoidance of membrane blockage, reduction of stripping solution volume and operational flexibility. The stability and mass‐transfer characteristics of two different membrane materials, poly(dimethylsiloxane) (PDMS) and ethylene–propylene diene terpolymer (EPDM), were tested in DCP solutions with different acidities in order to simulate real industrial waste streams. EPDM exhibits one order of magnitude lower mass‐transfer rates than PDMS (1.4 × 10 −7 m s −1 vs 20 × 10 −7 m s −1 at 30 °C and 2.4 × 10 −7 m s −1 vs 39 × 10 −7 m s −1 at 60 °C), however its higher resistance to acid attack provides higher membrane lifetimes. This can be crucial for MARS processes treating real acidic industrial wastewater. A 97% recovery of DCP with a water content of 15 wt% was obtained upon neutralisation of the stripping solution. Copyright © 2004 Society of Chemical Industry
Broadband dichromatic variational measurement (2021)

Standard Quantum Limit (SQL) of a classical mechanical force detection results from quantum back action impinged by the meter on a probe mechanical transducer perturbed by the force of interest. In this paper we introduce a technique of continuous \vy{broadband} back action avoiding measurements for the case of a resonant signal force acting on a linear mechanical oscillator supporting one of mirrors of an optical Michelson-Sagnac Interferometer (MSI). The interferometer with the movable mirror is an opto-mechanical transducer able to support polychromatic probe field. The method involves a dichromatic optical probe resonant with the MSI modes and having frequency separation equal to the mechanical frequency. We show that analyzing each of the harmonics of the probe reflected from the mechanical system separately and postprocessing the measurement results allows excluding the back action in a broad frequency band and measuring the force with sensitivity better than SQL.
Effects of Modafinil on Dopamine and Dopamine Transporters in the Male Human Brain (2009)

In this pilot study, modafinil blocked dopamine transporters and increased dopamine in the human brain (including the nucleus accumbens). Because drugs that increase dopamine in the nucleus accumbens have the potential for abuse, and considering the increasing use of modafinil, these results highlight the need for heightened awareness for potential abuse of and dependence on modafinil in vulnerable populations.
An Individualized Risk Calculator for Research in Prodromal Psychosis (2016)

A risk calculator comparable in accuracy to those for cardiovascular disease and cancer is available to predict individualized conversion risks in newly ascertained clinical high-risk cases. Given that the risk calculator can be validly applied only for patients who screen positive on the Structured Clinical Interview for Psychosis Risk Syndromes, which requires training to administer, its most immediate uses will be in research on psychosis risk factors and in research-driven clinical (prevention) trials.
Quantification of <sup>18</sup>F-JNJ-42259152, a Novel Phosphodiesterase 10A PET Tracer: Kinetic Modeling and Test–Retest Study in Human Brain (2013)

Kinetic modeling of (18)F-JNJ-42259152 shows that PDE10A activity can be reliably quantified and simplified using a reference tissue model with the frontal cortex as reference and a 60-min acquisition period.
Renouvellement des proteines et flux d'acides amines au cours du developpement. (1983)

ISBN 2-85340-479-X
Variables affecting production of monocyte chemotactic factor 1 from human leukocytes stimulated with Cryptococcus neoformans (1997)

The chemokine monocyte chemoattractant protein 1 (MCP-1) is produced predominantly by mononuclear phagocytes and stimulates recruitment into infected tissues of blood monocytes and T cells. These cell types are thought to be critical to host defenses against infections due to Cryptococcus neoformans, a major cause of disease in persons with AIDS and other disorders of cell-mediated immunity. Accordingly, in the present study, we examined the conditions under which human monocytes and bronchoalveolar macrophages (BAM) are stimulated by C. neoformans to produce MCP-1. C. neoformans was a potent inducer of MCP-1 release from monocytes, with levels of chemokine secreted similar to that seen following stimulation with lipopolysaccharide (LPS). BAM, in contrast, were stimulated by LPS, but not by C. neoformans, to secrete MCP-1. A peak in MCP-1 mRNA was seen 8 h following cryptococcal stimulation of monocytes. Nine strains of C. neoformans stimulated monocytes to release MCP-1, and there was only modest variation between strains. However, when an individual strain was used, the capacity of C. neoformans to stimulate monocyte MCP-1 release did vary, depending upon the conditions used to grow the fungal stimuli. Finally, C. neoformans stimulated comparable quantities of MCP-1 release in monocytes from donors with and without human immunodeficiency virus infection. These data establish C. neoformans as a potent stimulator of MCP-1 in monocytes, but not in BAM. The failure of C. neoformans to stimulate MCP-1 in BAM, if occurring in vivo, could result in a diminished cell-mediated inflammatory response following inhalation of airborne fungi.
Meta-clustering of gene expression data and literature-based information (2003)

The current tendency in the life sciences to spawn ever growing amounts of high-throughput assays has led to a situation where the interpretation of data and the formulation of hypotheses lag the pace at which information is produced. Although the first generation of statistical algorithms scrutinizing single, large-scale data sets found their way into the biological community, the great challenge to connect their results to existing knowledge still remains. Despite the fairly large number of biological databases that is currently available, a lot of relevant information is found in free-text format (such as textual annotations, scientific abstracts and full publications). In this paper we explore how an integrated analysis of expression data and literature-extracted information can reveal biologically meaningful clusters not identified when using microarray information alone. The joint analysis is validated in terms of transcriptional regulation.
Action Hierarchies in Description Logics. (1997)

Our project aims at the automatic generation of multilingual text for product maintenance and documentation from a structured knowledge representation formalized by means of plans in a description logic. These plans consist of one or more actions or other plans. This language independent representation is also designed to support the qualitative simulation of the documentation steps and to support the generation of multilingual text by offering action descriptions at different levels of detail for verbalization. This requires a hierarchical organization of performable action descriptions capable to define actions by inheriting already existing actions. Prerequisite for inheritance of pre- and postconditions of actions in a KL-ONE system is the definition of a proper subsumption relation for this logical expressions, conform with the subsumption for ordinary KLONE constructs. We propose a framework for extending the Loom system with syntax and semantics for representing and reasoning wi...
Large T1 contrast enhancement using superparamagnetic nanoparticles in ultra-low field MRI (2018)

Superparamagnetic iron oxide nanoparticles (SPIONs) are widely investigated and utilized as magnetic resonance imaging (MRI) contrast and therapy agents due to their large magnetic moments. Local field inhomogeneities caused by these high magnetic moments are used to generate T<sub>2</sub> contrast in clinical high-field MRI, resulting in signal loss (darker contrast). Here we present strong T<sub>1</sub> contrast enhancement (brighter contrast) from SPIONs (diameters from 11 nm to 22 nm) as observed in the ultra-low field (ULF) MRI at 0.13 mT. We have achieved a high longitudinal relaxivity for 18 nm SPION solutions, r<sub>1</sub> = 615 s<sup>-1</sup> mM<sup>-1</sup>, which is two orders of magnitude larger than typical commercial Gd-based T<sub>1</sub> contrast agents operating at high fields (1.5 T and 3 T). The significantly enhanced r<sub>1</sub> value at ultra-low fields is attributed to the coupling of proton spins with SPION magnetic fluctuations (Brownian and Néel) associated with a low frequency peak in the imaginary part of AC susceptibility (χ"). SPION-based T<sub>1</sub>-weighted ULF MRI has the advantages of enhanced signal, shorter imaging times, and iron-oxide-based nontoxic biocompatible agents. This approach shows promise to become a functional imaging technique, similar to PET, where low spatial resolution is compensated for by important functional information.
Proceedings of the 2003 Antenna Applications Symposium. Volume 2 (2003)

Abstract : The Proceedings of the 2003 Antenna Applications Symposium is a collection of state-of-the-art papers relating to antenna arrays, millimeter wave antennas, simulation and measurement of antennas, integrated antennas, and antenna bandwidth and radiation improvements.
Zero Botnets: An Observe-Pursue-Counter Approach (2022)

Adversarial Internet robots (botnets) represent a growing threat to the safe use and stability of the Internet. Botnets can play a role in launching adversary reconnaissance (scanning and phishing), influence operations (upvoting), and financing operations (ransomware, market manipulation, denial of service, spamming, and ad click fraud) while obfuscating tailored tactical operations. Reducing the presence of botnets on the Internet, with the aspirational target of zero, is a powerful vision for galvanizing policy action. Setting a global goal, encouraging international cooperation, creating incentives for improving networks, and supporting entities for botnet takedowns are among several policies that could advance this goal. These policies raise significant questions regarding proper authorities/access that cannot be answered in the abstract. Systems analysis has been widely used in other domains to achieve sufficient detail to enable these questions to be dealt with in concrete terms. Defeating botnets using an observe-pursue-counter architecture is analyzed, the technical feasibility is affirmed, and the authorities/access questions are significantly narrowed. Recommended next steps include: supporting the international botnet takedown community, expanding network observatories, enhancing the underlying network science at scale, conducting detailed systems analysis, and developing appropriate policy frameworks.
The effects of disruptions in ribosomal active sites and in intersubunit contacts on ribosomal degradation in Escherichia coli (2015)

Abstract Although ribosomes are very stable under most conditions, ribosomal degradation does occur in diverse groups of organisms in response to specific stresses or environmental conditions. While non-functional ribosome decay (NRD) in yeast is well characterized, very little is known of the mechanisms that initiate ribosomal degradation in bacteria. Here we test ribosome degradation in growing Escherichia coli expressing mutant ribosomes. We found that mutations in the 16S rRNA decoding centre (G530U and A1492C) and 23S rRNA active site (A2451G) do not lead to ribosomal degradation. In contrast, 23S rRNA mutation U2585A causes degradation of both the large and small ribosomal subunits in E. coli . We further tested mutations in 23S rRNA, which disrupt ribosomal intersubunit bridges B2a and B3. Deletion of helix 69 of 23S rRNA and the point mutation A1912G in the same helix did not destabilize ribosomes, while expression of mutations A1919G in H69 and A1960G in H71 led to degradation of both mutant and wild-type ribosomes. Our results suggest an actively induced mechanism requiring de novo protein synthesis for ribosomal degradation in E. coli , which degrades both structurally inactive and active ribosomes.
ChemInform Abstract: Bioinspired Organocatalytic Asymmetric Reactions. (2012)

Abstract Review: 152 refs.
Probiotics in Aquaculture : Potential for Sustainable Development (2011)

IJAR - Indian Journal of Applied Research (IJAR) IJAR is a double reviewed monthly print journal that accepts research works from scholars, academicians, professors, doctorates, lecturers, and corporate in their respective expertise of studies.
Pro-angiogenic and osteogenic composite scaffolds of fibrin, alginate and calcium phosphate for bone tissue engineering (2021)

Due to the limitations of bone autografts, we aimed to develop new composite biomaterials with pro-angiogenic and osteogenic properties to be used as scaffolds in bone tissue engineering applications. We used a porous, cross-linked and slowly biodegradable fibrin/alginate scaffold originally developed in our laboratory for wound healing, throughout which deposits of calcium phosphate (CaP) were evenly incorporated using an established biomimetic method. Material characterisation revealed the porous nature and confirmed the deposition of CaP precursor phases throughout the scaffolds. MC3T3-E1 cells adhered to the scaffolds, proliferated, migrated and differentiated down the osteogenic pathway during the culture period. Chick chorioallantoic membrane (CAM) assay results showed that the scaffolds were pro-angiogenic and biocompatible. The work presented here gave useful insights into the potential of these pro-angiogenic and osteogenic scaffolds for bone tissue engineering and merits further research in a pre-clinical model prior to its clinical translation.
The development of gut microbiota in critically ill extremely low birth weight infants assessed with 16S rRNA gene based sequencing (2014)

High prevalence and abundance of potentially pathogenic Enterobacteriaceae and Staphylococcaceae with low prevalence and abundance of colonization resistance providing taxa bifidobacteria, Bacteroides and lactobacilli may lead to high infection risk via microbial translocation from the gut. Additionally, our data suggest that maternal chorioamnionitis may have an effect on the diversity of infants' gut microbiota; however, the mechanisms involved remain to be elucidated.
Chloro(1,5-cyclooctadiene)(triphenylphosphine)rhodium(I) (2002)

The crystal and molecular structure of the title compound, [RhCl(C8H12){P(C6H5)3}], has been determined by means of X-ray diffraction. Compounds of this nature are important because of their ability to act as homogenous hydrogenation catalysts and to serve as precursors for more elaborate compounds.
Three dimensional elasticity approach for delamination buckling of composite plates (1997)

An three dimensional elasticity approach is presented for the delamination buckling of simply supported orthotropic and laminated composite plates. The uniform prebuckling stress assumption is made for composite laminates, which assumes the membrane state during prebuckling. The delaminated plate is divided into three regions and in each region the closed-form expressions for the displacements and stresses are derived. The nonlinear eigenvalue problem used to solve for the critical load is therefore constructed by using continuity equations at region interfaces and at layer interfaces. The results obtained from the elasticity solution are compared with the critical loads furnished by the classical laminate theory. The approach automatically accounts for the transverse shear and normal effects and provides a means of accurate assessment of various existing plate theories.
Genotyping of Verocytotoxin-Producing <i>Escherichia coli</i> O157: Comparison of Isolates of a Prevalent Phage Type by Fluorescent Amplified-Fragment Length Polymorphism and Pulsed-Field Gel Electrophoresis Analyses (2000)

We applied the high-resolution genotyping technique fluorescent amplified-fragment length polymorphism (FAFLP) analysis to 71 isolates of a single phage type (PT8) of pulsed-field gel electrophoresis (PFGE)-characterized verocytotoxin-producing Escherichia coli O157. Twenty-seven similar, but not identical, groupings were defined by both FAFLP analysis and the PFGE profiles. Given the FAFLP analysis conditions described here, these two methods exhibited equivalent discriminatory powers.
Supplementary Materials and Methods from AZD3514: A Small Molecule That Modulates Androgen Receptor Signaling and Function &lt;i&gt;In Vitro&lt;/i&gt; and &lt;i&gt;In Vivo&lt;/i&gt; (2023)

&lt;p&gt;PDF - 56KB, Supplementary methods detailing Relative peptide quantification by selected reaction monitoring (SRM)using mass spectrometry&lt;/p&gt;
In vivo activity of humanized monoclonal anti-EGFR antibody EMD 72000 in combination with gemcitabine on growth of primary pancreatic tumors and metastases in an orthotopic nude mouse model (2005)

Introduction: Deregulation of epidermal growth factor receptor (EGFR) pathways contributes to the progression of a wide range of cancers, and the EGFR is an attractive therapeutic target. The humanized EGFR-specific monoclonal antibody EMD 72000 has shown potent inhibitory activity in preclinical experiments and is under investigation in clinical studies.
Data from Delving into Early-onset Pancreatic Ductal Adenocarcinoma: How Does Age Fit In? (2023)

&lt;div&gt;AbstractPurpose:&lt;p&gt;With the rising incidence of early-onset pancreatic cancer (EOPC), molecular characteristics that distinguish early-onset pancreatic ductal adenocarcinoma (PDAC) tumors from those arising at a later age are not well understood.&lt;/p&gt;Experimental Design:&lt;p&gt;We performed bioinformatic analysis of genomic and transcriptomic data generated from 269 advanced (metastatic or locally advanced) and 277 resectable PDAC tumor samples. Patient samples were stratified into EOPC (age of onset ≤55 years; &lt;i&gt;n&lt;/i&gt; = 117), intermediate (age of onset 55–70 years; &lt;i&gt;n&lt;/i&gt; = 264), and average (age of onset ≥70 years; &lt;i&gt;n&lt;/i&gt; = 165) groups. Frequency of somatic mutations affecting genes commonly implicated in PDAC, as well as gene expression patterns, were compared between EOPC and all other groups.&lt;/p&gt;Results:&lt;p&gt;EOPC tumors showed significantly lower frequency of somatic single-nucleotide variant (SNV)/insertions/deletions (indel) in &lt;i&gt;CDKN2A&lt;/i&gt; (&lt;i&gt;P&lt;/i&gt; = 0.0017), and were more likely to achieve biallelic mutation of &lt;i&gt;CDKN2A&lt;/i&gt; through homozygous copy loss as opposed to heterozygous copy loss coupled with a loss-of-function SNV/indel mutation, the latter of which was more common for tumors with later ages of onset (&lt;i&gt;P&lt;/i&gt; = 1.5e-4). Transcription factor forkhead box protein C2 (&lt;i&gt;FOXC2&lt;/i&gt;) was significantly upregulated in EOPC tumors (&lt;i&gt;P&lt;/i&gt; = 0.032). Genes significantly correlated with &lt;i&gt;FOXC2&lt;/i&gt; in PDAC samples were enriched for gene sets related to epithelial-to-mesenchymal transition (EMT) and included &lt;i&gt;VIM&lt;/i&gt; (&lt;i&gt;P&lt;/i&gt; = 1.8e-8), &lt;i&gt;CDH11&lt;/i&gt; (&lt;i&gt;P&lt;/i&gt; = 6.5e-5), and &lt;i&gt;CDH2&lt;/i&gt; (&lt;i&gt;P&lt;/i&gt; = 2.4e-2).&lt;/p&gt;Conclusions:&lt;p&gt;Our comprehensive analysis of sequencing data generated from a large cohort of PDAC patient samples highlights a distinctive pattern of biallelic &lt;i&gt;CDKN2A&lt;/i&gt; mutation in EOPC tumors. Increased expression of &lt;i&gt;FOXC2&lt;/i&gt; in EOPC, with the correlation between &lt;i&gt;FOXC2&lt;/i&gt; and EMT pathways, represents novel molecular characteristics of EOPC.&lt;/p&gt;&lt;p&gt;&lt;i&gt;See related commentary by Lou, p. 8&lt;/i&gt;&lt;/p&gt;&lt;/div&gt;
Finite Element Particle-In-Cell Direct Simulation Monte Carlo Simulations of Optically Triggered Nanosecond Electrical Switches. (2019)

Abstract not provided.