gebhart/patrepeval huggingface dataset:
Crystallization of Proteins on Chip by Microdialysis for &lt;i&gt;In Situ&lt;/i&gt; X-ray Diffraction Studies (2021)

This protocol describes the manufacturing of reproducible and inexpensive microfluidic devices covering the whole pipeline for crystallizing proteins on-chip with the dialysis method and allowing in situ single-crystal or serial crystallography experiments at room temperature. The protocol details the fabrication process of the microchips, the manipulation of the on-chip crystallization experiments and the treatment of the in situ collected X-ray diffraction data for the structural elucidation of the protein sample. The main feature of this microfabrication procedure lies on the integration of a commercially available, semipermeable regenerated cellulose dialysis membrane in between two layers of the chip. The molecular weight cut-off of the embedded membrane varies depending on the molecular weight of the macromolecule and the precipitants. The device exploits the advantages of microfluidic technology, such as the use of minute volumes of samples (<1 µL) and fine tuning over transport phenomena. The chip coupled them with the dialysis method, providing precise and reversible control over the crystallization process and can be used for investigating phase diagrams of proteins at the microliter scale. The device is patterned using a photocurable thiolene-based resin with soft imprint lithography on an optically transparent polymeric substrate. Moreover, the background scattering of the materials composing the microchips and generating background noise was evaluated rendering the chip compatible for in situ X-ray diffraction experiments. Once protein crystals are grown on-chip up to an adequate size and population uniformity, the microchips can be directly mounted in front of the X-ray beam with the aid of a 3D printed holder. This approach addresses the challenges rising from the use of cryoprotectants and manual harvesting in conventional protein crystallography experiments through an easy and inexpensive manner. Complete X-ray diffraction data sets from multiple, isomorphous lysozyme crystals grown on-chip were collected at room temperature for structure determination.
NHC-Catalyzed Boron Conjugate Additions to α,β-UnsaturatedCarbonyl Compounds (2009)

A novel efficient method for the formation of C-B bonds is reported. This method is based on a metal-free N-heterocyclic carbene catalyzed conjugate addition of bis(pinacolato)diboron to cyclic and acyclic α,β-unsaturated carbonyls, including lactones, esters, and ketones.
Effect of Base/Collector Implant and Emitter-Poly Overlap on Low Frequency Noise in SiGe HBTs (2005)

Low frequency noise characteristics of npn SiGe heterojunction bipolar transistors are explored. Transistors with selectively implanted collector and higher doping in the extrinsic base are tested. Variable lengths of the emitter‐poly overlap are also studied to investigate their effect on the device noise.
Discharge Characteristics of AC Plasma Display Panel Prepared Using Vacuum Sealing Method (2008)

The base vacuum level achieved before loading the discharge gas is known to be an important parameter that affects both the address and sustain discharge characteristics in an AC plasma display panel (PDP), as a higher base vacuum level improves the discharge characteristics. Accordingly, the vacuum sealing method, which can enhance the base vacuum level, is adopted to enhance the MgO characteristics by reducing any residual gas impurity. The resulting changes in the address and sustain discharge characteristics, including the secondary electron coefficient, firing voltage, and dynamic voltage margin, are then compared with the results when using conventional atmospheric-pressure sealing for a 42-in ac PDP with a high Xe (11%) content. The vacuum sealing method was found to improve the secondary electron emission coefficient, lower the firing voltage, particularly under MgO cathode conditions, and increase the dynamic voltage margin. However, the vacuum sealing was also found to deteriorate the visible transmittance of the dielectric layer in the front panel. Nonetheless, the vacuum sealing process enabled the use of a higher Xe content, which is up to 17%, under a stable dynamic margin voltage, thereby improving both the luminance and luminous efficiencies of the AC PDP.
Handbook of the Pottery of the Egyptian Middle Kingdom. Vol. 1: Corpus Volume (2012)

Das Handbuch der Keramik des agyptischen Mittleren Reiches ist eine umfassende typologische Studie agyptischer Keramikgefase, die im Mittleren Reich (Mitte der 11. bis Mitte der 13. Dynastie, ca. 2000-1700 v.Chr.) hergestellt und in Agypten, Nubien und der Levante gefunden wurden.
Das Handbuch setzt sich aus zwei Banden zusammen: Band I, das „Corpus Volume“, ist eine typologische Studie, die nach Keramikformen strukturiert ist. Band II, das „Regional Volume“, beinhaltet Untersuchungen zu Keramik in ihren fundplatzspezifischen Kontexten.
Der Fokus des Handbuches liegt auf der chronologischen Dimension. Es wird versucht, moglichst feine Datierungen fur keramische Formengruppen zu erstellen. Die Quellen fur das hier vorgelegte Material sind nicht nur veroffentlichte Bucher, sondern auch unpublizierte Grabungsberichte und Studien zahlreicher Wissenschaftler. Desweiteren wurde eine grose Gruppe von Keramikgefasen aus musealen Bestanden analysiert und gezeichnet, um hier vorgestellt zu werden.
Band I, das Corpus, besteht aus vier Hauptklassen: I. Offene Gefase, II. Geschlossene Gefase, III. Keramik der Produktion und spezifischer Verwendung im Haushalt und IV. Miniatur- und Modellkeramik. Beim Offnen des Handbuches findet sich auf der linken Seite eine Tafel mit einer Landkarte Agyptens, einer Zeitschiene, einer Tabelle mit grundsatzlicher Information zu Waren, Oberflachenbehandlungen, Formaten, Kontexten und Verwendungen, sowie ein kurzer Text und eine Bibliographie zu dieser spezifischen Formgruppe. Die rechte Seite zeigt Zeichnungen von ausgewahlten Gefasen, wobei darauf geachtet wurde, alle formalen Varianten dieser Formengruppe auf einer oder mehreren Tafeln vorzustellen.
Design Of Minimum Volume Emc Input Filters For An Ultra Compact Three-phase Pwm Rectifier (2009)

A design procedure is proposed for the electromagnetic compatibility (EMC) filters to be included in a three-phase/-level PWM rectifier unit in order to fulfill EMC requirements related to conducted emissions. An analytical volumetric optimization is performed considering constraints given by electrical safety, power factor and damping of resonances. The procedure avoids the necessity of applying numerical optimization allowing the analytical calculation of the total filter volume as function of rated power and switching frequency. The experimental verification of the proposed procedure is presented through con- ducted emissions (CE) measurements in the designed system.
Una visión general sobre la correlación entre el zinc en la sangre, la ingesta de zinc, la suplementación de zinc y la densidad mineral ósea en los seres humanos (2021)

RESUMEN. Introduccin: En caso de deficiencia de zinc, se limitar la sntesis muscular y sea. Los estudios en humanos sobre zinc y salud sea son pocos y no se han publicado comentarios sobre este tema. Por lo tanto, el objetivo de esta revisin narrativa es considerar el estado de la tcnica sobre la correlacin entre el zinc en la sangre, la ingesta diaria de zinc, la suplementacin de zinc y la densidad mineral sea. Material y mtodos: Se realiz una revisin narrativa. Resultados: Esta revisin incluy 16 estudios elegibles: ocho se refieren al zinc en sangre; tres estudios se refieren a la ingesta de Zn y cinco estudios se refieren a la suplementacin de Zn. Conclusin: Los niveles de zinc en sangre parecen ser ms bajos en sujetos con patologa relacionada con el metabolismo seo. En cuanto a la ingesta diaria de zinc, una alta proporcin de la poblacin, ms de 20%, parece estar en riesgo de tener una ingesta inadecuada de zinc. La literatura sugiere que una ingesta insuficiente de zinc (menos de 3 mg/da) podra ser un factor de riesgo de fracturas y para el desarrollo de osteopenia y osteoporosis. La suplementacin con zinc (40-50 g/da) podra tener efectos beneficiosos sobre la salud sea para mantener la densidad mineral sea y una curacin ms rpida en caso ABSTRACT. Introduction: In case of zinc (Zn) deficiency, this mineral becomes a nutrient limiting muscle and bone synthesis. The study in humans on zinc and bone health are few and no reviews have been published on this topic. So, the aim of this narrative review was to consider the state of the art on the correlation between blood zinc, daily zinc intake, zinc supplementation and bone mineral density. Material and methods: A narrative review was performed. Results: This review included 16 eligible studies: eight studies concern Zn blood; three studies concern Zn intake and five studies concern Zn supplementation. Conclusion: Blood zinc levels seem to be lower in subjects with pathology related to bone metabolism. Regarding daily zinc intake, a high proportion of the population, more than 20%, seems to be at risk of having inadequate zinc intake. The literature suggests that an insufficient zinc intake (less than 3 mg/day) could be a risk factor for fractures and for development of osteopenia and osteoporosis. Zinc supplementation (40-50 g/day) could have beneficial effects on bone health in terms of maintaining bone mineral density and faster healing in the event of fractures, with even better results in situations of reduced intake zinc through food.
&lt;title&gt;Evaluation of ALA-induced PpIX as a photosensitizer for PDT in cats&lt;/title&gt; (1998)

Given exogenously, ALA defeats intrinsic regulatory feedback mechanisms allowing intracellular accumulation of protoporphyrin IX (PpIX), a highly efficient photosensitizer. In vivo, PpIX synthesis in neoplastic mammary tissues averages 20-fold higher than in normal mammary tissues. PpIX is retained intracellularly, unlike perivascular localization of other photosensitizers, and it is then cleared quickly from the body. In vitro, ALA induced PpIX production in our laboratory in 6 cell lines tested, including an established feline kidney cell line and dermal fibroblasts from primary skin biopsy explant, resulting in photosensitization. Fluorescent microscopy confirmed PpIX production in skin adnexae following ALA administration in a normal cat. To evaluate toxicity, three cats were treated with a single i.v. dose of ALA (either 100, 200, of 400 mg/kg) and followed for 7 days. Cats receiving 100 or 200 mg/kg ALA i.v. had elevated liver enzymes and bilirubin within 24 hours. Histopathology revealed hydropic changes in the liver and renal fibrosis. The cat receiving 400 mg/kg ALA intravenously had cutaneous flush, bradycardia and apnea associated with ALA administration; within 24 hours the cat was lethargic, anorectic and icteric. ALT, AST and bilirubin concentrations had increased significantly. At necropsy the liver had a prominent lobular pattern; histopathology revealed severe periportal hepatitis and splenic necrosis. Systemically administered ALA induces PpIX production, but toxicity may preclude its clinical application in the cat. PpIX levels seem to be more time dependent than those dependent at these three ALA doses and they are well beyond the saturation point for adequate PpIX conversion. The literature is scant regarding toxicity associated with parenteral administration of ALA.
Cell Therapies for Muscular Dystrophy (2008)

A specific type of satellite cell can engraft dystrophic tissue in a mouse model of Duchenne's muscular dystrophy and can improve the strength and duration of muscle contractility.
Multilayer Mie Scattering Model for Investigation of Intracellular Structural Changes in the Nucleolus and Cytoplasm (2011)

Light scattering from biological cells has been used for many years as a diagnostic tool. Several simulation methods of the scattering process were developed in the last decades in order to understand and predict the scattering patterns. We developed an analytical model of a multilayer spherical scattering cell. Here, we describe the model and show that the results obtained within this simple method are similar to those obtained with far more complicated methods such as finite-difference time-domain (FDTD). The multilayer model is then used to study the effects of changes in the distribution of internal cell structures like mitochondria distribution or nucleus internal structures that exist in biological cells. Such changes are related with cancerous processes within the cell as well as other cell pathologies. Results show the ability to discriminate between different cell stages related to the mitochondria distributions and to internal structure of the nucleolus.
Techniques for addressing fundamental privacy and disruption tradeoffs in awareness support systems (1996)

Article Free Access Share on Techniques for addressing fundamental privacy and disruption tradeoffs in awareness support systems Authors: Scott E. Hudson Graphics, Visualization, and Usability Center, and College of Computing, Georgia Institute of Technology, Atlanta, Georgia Graphics, Visualization, and Usability Center, and College of Computing, Georgia Institute of Technology, Atlanta, GeorgiaView Profile , Ian Smith Graphics, Visualization, and Usability Center, and College of Computing, Georgia Institute of Technology, Atlanta, Georgia Graphics, Visualization, and Usability Center, and College of Computing, Georgia Institute of Technology, Atlanta, GeorgiaView Profile Authors Info & Claims CSCW '96: Proceedings of the 1996 ACM conference on Computer supported cooperative workNovember 1996 Pages 248–257https://doi.org/10.1145/240080.240295Online:16 November 1996Publication History 202citation1,910DownloadsMetricsTotal Citations202Total Downloads1,910Last 12 Months91Last 6 weeks17 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Natural history of transient myeloproliferative disorder clinically diagnosed in Down syndrome neonates: a report from the Children's Oncology Group Study A2971 (2011)

Transient myeloproliferative disorder (TMD), restricted to newborns with trisomy 21, is a megakaryocytic leukemia that although lethal in some is distinguished by its spontaneous resolution. Later development of acute myeloid leukemia (AML) occurs in some. Prospective enrollment (n = 135) elucidated the natural history in Down syndrome (DS) patients diagnosed with TMD via the use of uniform monitoring and intervention guidelines. Prevalent at diagnosis were leukocytosis, peripheral blast exceeding marrow blast percentage, and hepatomegaly. Among those with life-threatening symptoms, most (n = 29/38; 76%) received intervention therapy until symptoms abated and then were monitored similarly. Organomegaly with cardiopulmonary compromise most frequently led to intervention (43%). Death occurred in 21% but only 10% were attributable to TMD (intervention vs observation patients: 13/14 vs 1/15 because of TMD). Among those solely observed, peripheral blasts and all other TMD symptoms cleared at a median of 36 and 49 days from diagnosis, respectively. On the basis of the diagnostic clinical findings of hepatomegaly with or without life-threatening symptoms, 3 groups were identified with differing survival: low risk with neither finding (38%), intermediate risk with hepatomegaly alone (40%), and high risk with both (21%; overall survival: 92% ± 8%, 77% ± 12%, and 51% ± 19%, respectively; P ≤ .001). Among all, AML subsequently occurred in 16% at a median of 441 days (range, 118-1085 days). The trial is registered at http://www.clinicaltrials.gov as NCT00003593.
Withdrawal: Letter to the Editor ‐ responses to Weisntein D, Simon M, Yehezkel E, Laron Z, Werner H. Insulin analogues display IGF‐I‐like mitogenic and antiactivity in cultured cancer cells (2013)

We thank the team of Eli Lilly and Company for their critical appraisal of our study and for giving us the opportunity to clarify a number of points [1]. We agree with Kazda et al. that randomized clinical trials will best address any direct relationship between insulin analogues and cancer in man. We do not question the fact that tight control of blood glucose has dramatically advanced the lives of patients with diabetes. This article is protected by copyright. All rights reserved.
A non-Euclidean phasor approach for distinction of fluorescent compounds using two-photon fluorescence lifetime imaging microscopy in ex vivo human skin (Conference Presentation) (2018)

Two-photon fluorescence lifetime imaging microscopy (FLIM) is a technique that not only probes the intensity of fluorophores, but also provides the temporal decay trace of said fluorophores on a pixel-by-pixel basis. These traces can then be transformed into the frequency domain for subsequent analysis, resulting in a scatterplot of phasor coordinates where each phasor corresponds to a single image pixel. With this in mind, it follows that individual fluorophores result in distinct clusters in the phasor plot, and a mixture of two fluorophores results in phasors that fall somewhere along a line linking the two clusters depending on the relative fluorophore concentrations. Until now, distinction of fluorescent species has relied mainly on computing the Euclidean distance between a given phasor and the mean coordinates of reference phasor clusters. However, this approach becomes inadequate in cases where one fluorophore has a much wider lifetime distribution than the other. As such, we propose the use of the Mahalanobis distance as an alternative to the Euclidean distance, as this metric additionally factors in the relative spread of each reference phasor cluster. This method has been applied to studying the oxidative response of ex vivo human skin via endogenous NADH fluorescence as it is exposed to chemical sun filters, the active ingredients in sunscreens. Given that both NADH and sun filters are fluorescent under the same excitation and emission conditions, the proposed Mahalanobis distance approach was used to distinguish the source of fluorescence in images of human skin. This allowed for the assessment of oxidative response as well as the tracking and monitoring of the sun filter formulation as it permeated throughout the skin.
An Image Quality Assessment Using Mean-Centered Weber Ratio and Saliency Map (2015)

Chen proposed an image quality assessment method to evaluate image quality at a ratio of noise in an image. However, Chen's method had some drawbacks that unnoticeable noise is reflected in the evaluation or noise position is not accurately detected. Therefore, in this paper, we propose a new image quality measurement scheme using the mean-centered WLNI (Weber's Law Noise Identifier) and the saliency map. The experimental results show that the proposed method outperforms Chen's and agrees more consistently with human visual judgment.
A Highly Linear and Efficient 28-GHz PA With a <i>P</i> <sub>sat</sub> of 23.2 dBm, <i>P</i> <sub>1 dB</sub> of 22.7 dBm, and PAE of 35.5% in 65-nm Bulk CMOS (2020)

This article presents a 28-GHz power amplifier (PA) suitable for fifth-generation (5G) wireless systems. Designed in a 65-nm bulk CMOS, the PA achieves a saturated output power (P <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">sat</sub> ) of 23.2 dBm at 28 GHz. The AM-AM and the AM-PM distortion have the overshoot of less than 0.3 dB and 0.2°, respectively, at 28 GHz. The AM-PM variation remains less than 1.9° over the frequency band of 26-31 GHz with a peak power-added efficiency (PAE) of 35.5%. Several techniques, including strategic placement of varactors, a proposed multi-function coplanar-waveguide (CPW)-like power combining structure, and a systematic design approach for the passive networks, are used to improve the efficiency and linearity of the design leading to a negligible difference between P <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sub> <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">dB</sub> and P <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">sat</sub> . The proposed linearity improvement technique, which uses an adaptive feedback structure to adjust the input impedance of the output stage based on the output power and also direct more current at higher output power levels, enhances the linearity through lowering both AM-AM and AM-PM conversions. The design enables 2.5-GS/s 64-QAM operation at average P <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">out</sub> of 16.1 dBm with an error-vector magnitude/adjacent-channel power ratio (EVM/ACPR) of better than -26.2-dB/-28.32-dBc without using any digital pre-distortion.
Antibacterial Activity of Lactophoricin, a Synthetic 23-Residues Peptide Derived from the Sequence of Bovine Milk Component-3 of Proteose Peptone (2004)

A synthetic peptide of 23 residues corresponding to the carboxyterminal 113 to 135 region of component-3 of proteose peptone (PP3) has been investigated with regard to its antibacterial properties. This cationic amphipathic peptide that we refer to as lactophoricin, displayed a growth-inhibitory activity against both gram-positive and gram-negative bacteria. For most of the strains tested, bacterial growth was observed in the presence of lactophoricin except for Streptococcus thermophilus. In that case, lactophoricin exhibited a minimum inhibitory concentration of 10 microM and a minimum lethal concentration of 20 microM. No hemolysis of human red blood cells was detected for peptide concentrations between 2 to 200 microM, indicating that lactophoricin would be noncytotoxic when used in this concentration range.
Implications of Integrated CPU-GPU Processors on Thermal and Power Management Techniques (2018)

Heterogeneous processors with architecturally different cores (CPU and GPU) integrated on the same die lead to new challenges and opportunities for thermal and power management techniques because of shared thermal/power budgets between these cores. In this paper, we show that new parallel programming paradigms (e.g., OpenCL) for CPU-GPU processors create a tighter coupling between the workload, the thermal/power management unit and the operating system. Using detailed thermal and power maps of the die from infrared imaging, we demonstrate that in contrast to traditional multi-core CPUs, heterogeneous processors exhibit higher coupled behavior for dynamic voltage and frequency scaling and workload scheduling, in terms of their effect on performance, power, and temperature. Further, we show that by taking the differences in core architectures and relative proximity of different computing cores on the die into consideration, better scheduling schemes could be implemented to reduce both the power density and peak temperature of the die. The findings presented in the paper can be used to improve thermal and power efficiency of heterogeneous CPU-GPU processors.