gebhart/patrepeval huggingface dataset:
Purification and Characterization of N-Acetylglucosamine-6-sulfate Sulfatase from Bovine Kidney: Evidence for the Presence of a Novel Endosulfatase Activity (1994)

N-Acetylglucosamine-6-sulfate sulfatase (NG6SS) is an enzyme that catalyzes the hydrolysis of sulfate esters from the C-6 hydroxyl of N-acetylglucosamine. We report our purification and characterization of the enzyme and the discovery that it can remove sulfate from internally sulfated GlcNAc on glycopeptides and glycoproteins. The enzyme was purified from bovine kidney over 200,000-fold using a combination of ion-exchange and size-exclusion chromatography. NG6SS is soluble and occurs as a single subunit with apparent solution molecular weight of 60.2 kDa on gel filtration chromatography and approximately 52.5 and 57.8 kDa on reducing and nonreducing SDS/PAGE, respectively. The enzyme is highly basic and exhibits a broad pH range with an optimum at pH 6.5 and a temperature optimum of 37 degrees C. Among the mono- and disaccharide sulfates tested, only GlcNAc-6-SO4 is an effective substrate with a Km of 4.7 mM, and either free sulfate or phosphate inhibits the activity. Unexpectedly, we found that the enzyme displays endosulfatase activity and quantitatively releases 35SO4 from 35SO4-labeled glycopeptides and intact glycoproteins isolated from human Molt-3 cells, which we have previously shown to synthesize glycoproteins containing GlcNAc-6-SO4 residues within the sequence Gal beta 1-4[SO-3-6]-GlcNAc beta 1-R of complex-type N-linked oligosaccharides. The N-terminal sequence of the bovine NG6SS was homologous to a human-liver-derived N-acetylglucosamine-6-sulfatase. The endosulfatase activity of bovine kidney NG6SS may be important in its potential role in the degradation of sulfated glycans and may make this enzyme a valuable reagent to study the biological functions of sulfated glycoproteins.
Doping end-point of the pseudogap in the superconducting phase of Bi2Sr2CaCu2O8+d cuprate (2017)

Combining electronic Raman scattering experiments with cellular dynamical mean field theory, we present evidence of the pseudogap in the superconducting state of various hole-doped cuprates. In Bi2Sr2CaCu2O8+d we track the superconducting pseudogap hallmark, a peak-dip feature, as a function of doping p. The pseudogap reduces on the overdoped side, but it survives well above the optimal doping, and experimentally disappears above p=0.22, the same doping level where the normal-state pseudogap collapses at a Lifshitz transition. This suggests that the pseudogap end-point in the (T-p) phase diagram is a vertical line.
Neonatal Immunization with Respiratory Syncytial Virus Glycoprotein Fragment Induces Protective Immunity in the Presence of Maternal Antibodies in Mice (2013)

Respiratory syncytial virus (RSV) is a major cause of severe lower respiratory tract infections in infants and the elderly worldwide. The significant morbidity and mortality associated with this infection underscores the urgent need for development of RSV vaccine. In this study, we first show that intranasal administration of RSV glycoprotein core fragment (Gcf) to neonatal mice can induce systemic humoral immune responses and protective immunity against RSV without causing lung eosinophilia, although antibody response was shifted to a Th2 response. Next, we examined whether the presence of maternal anti-RSV antibodies would affect the responsiveness and protection efficacy of Gcf in newborn mice, since infants can possess RSV-specific maternal antibodies due to frequent RSV re-infections to adults. Intranasal administration of Gcf induced antibody response and increased IFNγ secretion and protected mice against RSV challenge without severe lung eosinophilia, even in the presence of high levels of RSV-specific maternal antibodies. Thus, our findings suggest that Gcf may be an effective and safe RSV vaccine during the neonatal period.
APPENDIX E Complete Nucleotide Sequences of Three Retroviral Genomes and a Cellular onc Gene (1982)

Three retroviral genomes have been entirely sequenced: Rous sarcoma virus (RSV), Moloney murine leukemia virus (Mo-MLV), and Moloney murine sarcoma virus (Mo-MSV). In addition, the complete nucleotide sequence of the cellular homolog of the oncogene carried by Mo-MSV, c-mos , has been determined. In all cases, the sequence of only one strand, the coding strand, is provided here. The sequences are written for a DNA copy—that is, Ts are used instead of Us. The proposed amino acid sequences for known genes are given; also, correlations with protein sequence will be discussed briefly in the legends for the individual sequences and in the section devoted to amino acid sequence (Appendix F). 1. The Complete Nucleotide Sequence of the Pr-C Strain of Rous Sarcoma Virus The sequence is presented as a DNA version of the entire viral RNA genome. The sequence begins with the R sequence “R” at the 5′ end of the genome. The precise boundary between R and U 5 (here marked with an arrow) is dependent on the extent of the R sequence at the 3′ end of viral RNA (see Appendix D). The 3′ end of U 5 forms an imperfect inverted repeat with U 3 (12 of 15 bases); inverted repeats of this sort are present in the LTRs of all retroviruses (see Appendix D). A horizontal arrow marks the inverted repeat; the mismatched bases are indicated by boxes. Just beyond U 5 is the primer binding site (PBS). Approximately 280 bases beyond U 5 is the beginning of the gag coding...
MITK Diffusion Imaging (2012)

The open source release of the modular MITK-DI tools will increase verifiability and comparability within the research community and will also be an important step towards bringing many of the current techniques towards clinical application.
Hypoglycaemia‐induced changes in regional brain volume and memory function (2013)

This case study highlights the potential of hypoglycaemia for permanent deleterious effects on brain structure and memory function. Our results suggest that subcortical grey matter, periventricular white matter and posterior white matter may be most susceptible to injury from hypoglycaemia exposure, and that structural damage to the hippocampus and isthmus of the cingulate may play a central role in hypoglycaemia-induced memory impairments.
GENETIC EXCHANGE IN BIOFILMS (2004)

Horizontal gene transfer is one of the principal mechanisms driving the evolution of microorganisms. One can induce from this “archeological evidence” that genetic exchange between bacteria occurs fairly frequently. This chapter describes the examples of genetic exchange in biofilms as they have been observed in the laboratory. Transformation in biofilms has, to date, only been demonstrated in two main in vitro model systems. Quorum sensing in gram-positive bacteria has been found to regulate a number of physiologic activities in different species, including competence development in Bacillus subtilis, Streptococcus mutans, Streptococcus gordonii, and Streptococcus pneumoniae. Optimal transformation frequencies were observed when fresh medium was fed to the biofilms, and the amount of surrounding planktonic cells was limited. The high rates of transformation observed with both S. mutans and Acinetobacter biofilms suggest that competence may be the normal state for surface-adherent populations. In addition to increasing the probability of donor-recipient contact it has been suggested that genes activated by contact with surfaces may be involved in the process of conjugation. To visualize actual mating events in biofilms, samples were examined by scanning electron microscopy. Furthermore, the fact that bacteria that are capable of forming biofilms are generally adept at genetic exchange should give us the impetus to study these processes in detail. A means of controlling genetic exchange in biofilms may help us not only to limit the transfer of undesirable characteristics such as antibiotic resistance in medical infections, but also to enhance the desirable processes in other applications.
Clinical Images: Postterm Newborn with Lissencephaly Presented with Seizure: Case Report and Review of Literature. (2015)

Malformations of cortical development include a wide range of anomalies that commonly lead to developmental delay and epilepsy. Lissencephaly, literally “smooth brain,” is a rare genetic brain malformation characterized by the absence of normal convolutions (folds) in the cerebral cortex. A
Measurement-based evaluation of antenna- and time diversity for mobile satellite systems (2011)

Signal diversity is used as a common method to improve the service availability in digital satellite communication and broadcasting systems. This paper focuses on the achievable receive antenna- and time diversity gain for land mobile satellite applications in various environments. A measurement van was equipped with four antennas, mounted at the front, back, left and right side of the rooftop. A high-power satellite broadcasting signal within the S-band was recorded along the east coast of the United States. The diversity gain is calculated from the first-order statistics of the measured high-resolution fading signals.
Motion estimation and compensation for CIF/SIF video sequences (2002)

The non-uniform sampling theory states that any analogue signal whose spectrum is limited to 1/T can be exactly recovered from 2 sequences of samples taken at a rate 1/T and having a different sampling phase. This paper addresses the problem of motion estimation and compensation of CIF/SIF sequences when aliasing is still existing within the frames. This is the case when the method promoted by MPEG 4 is used to obtain CIF/SIF sequences from CCIR 601 original sequences. The motion estimation is performed by using the two previous frames instead of only one previous frame. Interpolation formulas are derived from the non-uniform sampling theory and simulation results are furnished.
Functional, endogenously expressed corticotropin‐releasing factor receptor type 1 (CRF<sub>1</sub>) and CRF<sub>1</sub>receptor mRNA expression in human neuroblastoma SH‐SY5Y cells (1999)

Corticotropin-releasing factor (CRF) is a hypothalamic 41-amino acid peptide which stimulates corticotropin (ACTH) release from the anterior pituitary and is also involved in the body response to stress. CRF1 receptors represent a potential target for novel antidepressant/anxiolytic drugs. The aim of the present study was to search for a human cell line expressing native, functional CRF1 receptors as a starting material for screening purposes. We identified CRF1 receptors functionally coupled to cAMP formation in human neuroblastoma SH-SY5Y cells. CRF induced concentration-dependent increases in cAMP accumulation in SH-SY5Y cells (maximal increase 6.9 +/- 0.9 fold over basal values, n = 14). This effect was mimicked by related peptides with similar potencies: (mean pEC50 value) human/rat CRF (8.63), rat urocortin (9.32), sauvagine (8.97), urotensin I (8.93), ovine CRF (8.81). The efficacies of these agonists were nearly the same, with the exception of ovine CRF which was slightly less efficacious (75% the Emax of CRF). The responses to CRF were competitively antagonised by the following peptide fragments (mean pKB value): alpha-helical-CRF (9-41) (7.54), [D-Phe12,Nle21,38,C alpha MeLeu37]CRF (12-41) (8.36) and [D-Tyr12]astressin (9.49) and by the selective, non-peptidic CRF1 receptor antagonists, CP-154,526 (7.76) and antalarmin (9.19). Estimation of receptor density by [125I]Tyr0-ovine CRF saturation binding yielded a modest number of binding sites (Bmax 12 fmol/mg protein, KD 0.2 nM). Analysis of mRNA by reverse transcription-polymerase chain reaction clearly revealed the presence of mRNA for CRF1 receptors in SH-SY5Y cells. A slight signal for CRF2 receptor mRNA was also observed. We conclude that neuroblastoma SH-SY5Y cells are endowed with native CRF1 receptors positively coupled to cAMP formation. They therefore constitute a useful functional model for the search of CRF1 selective compounds with potential anxiolytic/antidepressant activity.
A cache error propagation model (2002)

Cache memory is a small, fast, memory system that holds frequently used data. With increasing processor speed, aggressive design practices increase the probability of fault occurrence and the presence of latent errors as the processor allows a short duration for read and write. The fault may corrupt the cache memory system or lead to an erroneous internal CPU state. The authors investigate error propagation in the cache memory system due to transient faults either in the cache memory itself or in the processor's registers or both. The information gained from such an investigation should lead to the development of more effective error recovery mechanisms against failures due to transient faults arising in the machine's cache memory and register set. They establish that even though the computer system is capable of recovering about 50% of the time from the effect of a single erroneous cache location/processor register, the other 50% of the time error recovery is affected only through specific recovery mechanisms. Their results are obtained using both a discrete-time Markov model and by means of error injection on a real system.
Role of Liquid Concentration in Coke Yield from Model Vacuum Residue–Coke Agglomerates (2015)

The fluid coking process is an example of an upgrading process that uses hot solids to heat and crack bitumen into more valuable products. When feed liquid is sprayed into a fluid bed of hot solids, the solids tend to agglomerate, giving simultaneous heating, reaction, and disintegration processes. The reaction of mixtures of three Athabasca vacuum residues and fluid coke particles was investigated by heating them in Curie point reactors in an induction furnace up to 530 °C. Small scale reactors with machined wells were fabricated from Curie point alloy. The yield of coke was measured as a function of the ratio of liquid to solid, heating rate, and feed type. Coke yields were insensitive to heating rates from 5 to 120 °C/s, at a constant final temperature. Bubbling was observed as the ratio of liquid feed in the mixture was raised above a threshold, depending on the reactivity of the feed used. Bubbling was observed to increase with greater heating rates, but it had little effect on ultimate coke yield. Coke yield increased with the fraction of bitumen on particles for all feed types tested. When the coke yields were normalized using the microcarbon residue content of the feeds, the results did not give a single trend when plotted with fraction of feed present in the mixtures.
Septisch-toxisches Herz-Kreislauf-Versagen als Folge einer Heparin-induzierten Thrombozytopenie mit „White-Clot-Syndrome” (1993)

A patient is presented who developed pulmonary artery embolism and thrombocytopenia with various arterial clots several days after an ankle joint osteosynthesis, which finally caused the amputation of the right arm. Unfractionated heparin was applied for the prevention of postoperative thrombosis. Obviously as a consequence of the liberation of toxic metabolites from the gangrenous lower arm, the patient developed septic shock which was exacerbated by a severe pneumonia caused by methicillin-resistant S. aureus bacteria (MRSA). Laboratory investigations showed an antibody (IgG) induced thrombopenia mediated by all low molecular weight and unfractionated heparins. By eliminating heparin and further treatment with a low molecular weight heparinoid (ORG 10,172), platelet count rose sufficiently and thrombotic process ceased completely. Septic shock was successfully treated by fluid replacement, antibiotics and inotropic substances.
Identification and control of piezoelectric benders for skin mechanical impedance estimation (2021)

This paper presents an integrated probe, designed to measure the rheological properties of the skin in situ. It includes two piezoelectric bender actuators and strain gauges as sensors. The advantage of the proposed probe is that the measurements of tip force and displacement are accomplished without external devices. A feedback voltage control is applied to control the vibration amplitude of the piezoelectric benders. Through feedback from integrated strain gauges, the displacement control is achieved. As shown in the simulation, the closed-loop system is robust to disturbance and uncertainty. The proposed probe may be used to measure skin mechanical impedance.
Generation of glucocorticoid resistant SARS-CoV-2 T-cells for adoptive cell therapy (2020)

Adoptive cell therapy with viral-specific T cells has been successfully used to treat life-threatening viral infections, supporting the application of this approach against COVID-19. We expanded SARS-CoV-2 T-cells from the peripheral blood of COVID-19-recovered donors and non-exposed controls using different culture conditions. We observed that the choice of cytokines modulates the expansion, phenotype and hierarchy of antigenic recognition by SARS-CoV-2 T-cells. Culture with IL-2/4/7 but not other cytokine-driven conditions resulted in >1000 fold expansion in SARS-CoV-2 T-cells with a retained phenotype, function and hierarchy of antigenic recognition when compared to baseline (pre-expansion) samples. Expanded CTLs were directed against structural SARS-CoV-2 proteins, including the receptor-binding domain of Spike. SARS-CoV-2 T-cells could not be efficiently expanded from the peripheral blood of non-exposed controls. Since corticosteroids are used for the management of severe COVID-19, we developed an efficient strategy to inactivate the glucocorticoid receptor gene ( <i>NR3C1</i> ) in SARS-CoV-2 CTLs using CRISPR-Cas9 gene editing.
An experimental study on UWB BPF loaded with dielectric rods (2008)

In this study, a new BPF is proposed by combining the periodic dielectric structure and the short-ended stubs. First, the analytical results showed that the high dielectric rods implanted in the dielectric substrate provides a stopband without affecting the passband which the short-ended stubs produce. Next, the proposed BPF is fabricated by using the LTCC technology. Lastly, the fabricated BPF's transmission characteristics are compared with the analytical results. As a result, the proposed BPF largely realized the transmission characteristics which fulfill the mask of FCC-Full band.
Preparation and characterization of the copper(II) and zinc(II) complexes of D-.beta.-(2-pyridyl)-.alpha.-alaninate and their condensation products with acetone (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPreparation and characterization of the copper(II) and zinc(II) complexes of D-.beta.-(2-pyridyl)-.alpha.-alaninate and their condensation products with acetoneStephen R. Ebner and Robert J. AngeliciCite this: Inorg. Chem. 1981, 20, 9, 2971–2977Publication Date (Print):September 1, 1981Publication History Published online1 May 2002Published inissue 1 September 1981https://pubs.acs.org/doi/10.1021/ic50223a043https://doi.org/10.1021/ic50223a043research-articleACS PublicationsRequest reuse permissionsArticle Views48Altmetric-Citations9LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Large-FSR Thermally Tunable Double-Ring Filters for WDM Applications in Silicon Photonics (2017)

We present the design procedure and experimental results of thermally tunable double ring resonators for integrated wavelength division multiplexing applications. A detailed analytical model specific for double rings is described, and a modified racetrack geometry using Bezier bends is used to reduce bending loss. We demonstrate devices with a free-spectral-range up to 2.4 THz (19 nm) around 1550 nm and nonadjacent channel rejection higher than 35 dB. The experimental results of thermally tunable double ring resonators is also presented with doped silicon integrated heaters, allowing the device to be used as a tunable filter or a switch.
Gas Cluster Ion Beam (GCIB) surface modification of titanium enhances osteoblast proliferation and bone formation <i>in vitro</i> (2010)

Titanium implants are considered to be bioactive, but osseointegration is often slow. Many have increased surface area of the implant to which osteoblasts bind by etching or sandblasting, and applied additive coatings such as hydroxyapatite. We have altered the atomic‐level structure of the TiO 2 surface without adding material onto the surface. GCIB utilizes high energy ionized gas clusters of inert argon atoms. Bombardment of TiO 2 results in increased surface wettability and amorphization. Ti pieces were divided into 2 groups: untreated, or GCIB irradiated with 5×10 14 Ar clusters/cm 2 . Osteoblasts were seeded at 2000 cells/cm 2 , allowed to attach and proliferate up to 10 days (n=3). Cell counts were measured by MTS assay. RNA was extracted over 10 days and amplified for alkaline phosphatase (ALPL). Protein was extracted at 4, 24, and 48h and probed for total and phosphorylated p42, as a marker for cell proliferation. GCIB‐treated TiO 2 showed: increased proliferation by day 10 (12,213 ± 1,570 vs 6,880 ±700 cells p&lt;0.05); ALPL upregulated by 3.4 ± 0.6 (p&lt;0.01) fold by day 10, indicating bone formation; phosphorylation of p42 is seen at 4h and sustained at 24h on GCIB‐treated, and only 4h on non‐treated surfaces. Results suggest that osteoblasts adhere and proliferate better on GCIB‐treated TiO 2 . GCIB treatment of dental implants has potential to enhance bone formation and significantly decrease osseointegration time.