gebhart/patrepeval huggingface dataset:
Circulating H3K27 Methylated Nucleosome Plasma Concentration: Synergistic Information with Circulating Tumor DNA Molecular Profiling (2023)

The molecular profiling of circulating tumor DNA (ctDNA) is a helpful tool not only in cancer treatment, but also in the early detection of relapse. However, the clinical interpretation of a ctDNA negative result remains challenging. The characterization of circulating nucleosomes (carrying cell-free DNA) and associated epigenetic modifications (playing a key role in the tumorigenesis of different cancers) may provide useful information for patient management, by supporting the contributive value of ctDNA molecular profiling. Significantly elevated concentrations of H3K27Me3 nucleosomes were found in plasmas at the diagnosis, and during the follow-up, of NSCLC patients, compared to healthy donors (<i>p</i>-value < 0.0001). By combining the H3K27Me3 level and the ctDNA molecular profile, we found that 25.5% of the patients had H3K27Me3 levels above the cut off, and no somatic alteration was detected at diagnosis. This strongly supports the presence of non-mutated ctDNA in the corresponding plasma. During the patient follow-up, a high H3K27Me3-nucleosome level was found in 15.1% of the sample, despite no somatic mutations being detected, allowing the identification of disease progression from 43.1% to 58.2% over molecular profiling alone. Measuring H3K27Me3-nucleosome levels in combination with ctDNA molecular profiling may improve confidence in the negative molecular result for cfDNA in lung cancer at diagnosis, and may also be a promising biomarker for molecular residual disease (MRD) monitoring, during and/or after treatment.
08: Kognitive Systeme, Vorlesung, SS 2019, 03.06.2019 (2019)

08 | 0:00:00 Start 0:01:37 Wiederholung 0:04:54 Neural Networks 0:23:42 Performance 0:28:20 Using Neural Nets 0:35:44 Generalization 0:43:04 Neural Nets-Modeling 0:45:59 Speech 0:50:55 A few Related Sciences 0:53:58 Anatomy of speech Production 1:09:21 Different vocal Tract Shapes 1:12:57 Formants
Experimental measurement of the wall-plug efficiency in THz quantum cascade lasers (2007)

The wall-plug efficiency and the thermal resistance of bound-to-continuum THz quantum-cascade lasers are extracted from the analysis of micro-probe photoluminescence spectra, via the direct measurement of the lattice temperature as a function of the electrical-power.
Staining and quantification of poly‐3‐hydroxybutyrate in <i>Saccharomyces cerevisiae</i> and <i>Cupriavidus necator</i> cell populations using automated flow cytometry (2005)

BODIPY 493/503 produced an overall better staining for PHB than did Nile red. When combined with the automated system, this technique provides a new method for the online monitoring and control of bioreactors.
Influence of Excess Loop Delay on the STF of Continuous-Time Delta-Sigma Modulators (2021)

This paper analyzes the effect of signal transfer function (STF) peaking in continuous-time (CT) Delta-Sigma- Modulators (DSMs) arising from excess loop delay (ELD) and its compensation. Starting from a generic model, the origin of different contributors to STF peaking are revised, namely noise transfer function (NTF) peaking due to aggressive noise shaping, instability from uncompensated delay as well as forward loop filter zeros in cascade-of-integrators with distributed feedforward (CIFF) modulators. The shift of forward loop filter zeros as a result of ELD compensation is shown intuitively for a 3rd order example, while system-level simulations of various 2nd, 3rd and 4th order CT single-loop modulators confirm increased STF peaking in the above stated scenarios.
Topical palmitoyl pentapeptide provides improvement in photoaged human facial skin<sup>1</sup> (2005)

The palmitoyl pentapeptide palmitoyl-lysine-threonine-threonine-lysine-serine (pal-KTTKS) is a synthetic material that was designed as a topical agent to stimulate collagen production and thus provide a skin anti-wrinkle benefit. To determine if pal-KTTKS is effective, the clinical study reported here was conducted. Caucasian female subjects (n = 93, aged 35-55) participated in a 12-week, double-blind, placebo-controlled, split-face, left-right randomized clinical study assessing two topical products: moisturizer control product vs. the same moisturizer product containing 3 ppm pal-KTTKS. Pal-KTTKS was well tolerated by the skin and provided significant improvement vs. placebo control for reduction in wrinkles/fine lines by both quantitative technical and expert grader image analysis. In self-assessments, subjects also reported significant fine line/wrinkle improvements and noted directional effects for other facial improvement parameters.
Emotional stressors trigger cardiovascular events (2012)

Therapies targeting this pathway can potentially prevent acute mental stressors from initiating plaque rupture. Limited evidence suggests that appropriately timed administration of beta-blockers, statins and aspirin might reduce the incidence of triggered myocardial infarctions. Stress management and transcendental meditation warrant further study.
Experimental Protocol for HOR and ORR in Alkaline Electrochemical Measurements (2018)

This work revises the essentials of electrocatalytic electrochemical measurement done in alkaline electrolytes. The hydrogen oxidation reaction (HOR) and the oxygen reduction reaction (ORR) were considered as a baseline to understand some key critical factors in a half-cell system. The material considered herein was essentially the carbon supported platinum nanoparticles as benchmark material. The main electrochemical techniques considered were cyclic voltammetry (CV) and the rotating disk electrode (RDE). The critical factors considered were the electrochemical cell vessel, reference electrode, nature of the cation in the alkaline electrolyte, and ohmic drop correction.
Online Diagnosis of PEMFC by Combining Support Vector Machine and Fluidic Mode (2014)

This paper deals with the online fault diagnosis of polymer electrolyte membrane fuel cell (PEMFC) stack. In the proposed approach, support vector machine (SVM) and a fluidic model are correlated to realize the diagnosis of the faults on water management. With the help of the fluidic model, health states of the stack can be identified. This procedure is then dedicated to labeling the training data, which is used to train the dimension reduction model, named Fisher discriminant analysis (FDA), and the SVM classifier. The online diagnosis can then be realized by using the trained FDA and SVM models. The proposed approach is illustrated by using the experimental data of a 20-cell PEMFC stack. The feasibility of the approach for online implementation is also affirmed.
Vigabatrin in the Treatment of Childhood Epilepsies: A Single‐Blind Placebo‐Controlled Study (1989)

Sixty-one pediatric patients (12-229 months of age) with refractory epilepsy were treated with vigabatrin [gamma-vinyl GABA (GVG)] in a 16-week, single-blind, add-on, placebo-controlled trial. Twenty-three patients (38%) showed a reduction of more than 50% in seizure frequency; 12 patients (20%) experienced a seizure increase; and the remaining 26 did not show significant differences between placebo and GVG treatment. Among the 216 patients who entered the long-term phase after having experienced more than 50% decrease in seizure frequency, 14 continued with the same degree of improvement after 2-11 months of follow-up (mean 7.7). GVG was particularly efficient in cryptogenic partial epilepsy. Conversely, nonprogressive myoclonic epilepsy tended to be aggravated. Agitation was the most commonly observed side effect, mainly at onset of therapy in mentally retarded patients, but was easily reversed by dose reduction. GVG is a promising drug in the treatment of refractory epilepsies of childhood.
An autoactive <i>NB-LRR</i> gene causes <i>Rht13</i> dwarfism in wheat (2022)

Semidwarfing genes have greatly increased wheat yields globally, yet the widely used gibberellin (GA)-insensitive genes <i>Rht-B1b</i> and <i>Rht-D1b</i> have disadvantages for seedling emergence. Use of the GA-sensitive semidwarfing gene <i>Rht13</i> avoids this pleiotropic effect. Here, we show that <i>Rht13</i> encodes a <i>nucleotide-binding site/leucine-rich repeat</i> (<i>NB-LRR</i>) gene. A point mutation in the semidwarf <i>Rht-B13b</i> allele autoactivates the <i>NB-LRR</i> gene and causes a height reduction comparable with <i>Rht-B1b</i> and <i>Rht-D1b</i> in diverse genetic backgrounds. The autoactive <i>Rht-B13b</i> allele leads to transcriptional up-regulation of pathogenesis-related genes including class III peroxidases associated with cell wall remodeling. <i>Rht13</i> represents a new class of reduced height (<i>Rht</i>) gene, unlike other <i>Rht</i> genes, which encode components of the GA signaling or metabolic pathways. This discovery opens avenues to use autoactive <i>NB-LRR</i> genes as semidwarfing genes in a range of crop species, and to apply <i>Rht13</i> in wheat breeding programs using a perfect genetic marker.
Reactions of (benzothiazol-2-ylthio)(trimethylsilyl)methane. A general method for .alpha.-mercaptoalkylation by alkylation and alkylative desilylation (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTReactions of (benzothiazol-2-ylthio)(trimethylsilyl)methane. A general method for .alpha.-mercaptoalkylation by alkylation and alkylative desilylationAlan R. Katritzky, Wojciech Kuzmierkiewicz, and Jose M. AurrecoecheaCite this: J. Org. Chem. 1987, 52, 5, 844–849Publication Date (Print):March 1, 1987Publication History Published online1 May 2002Published inissue 1 March 1987https://pubs.acs.org/doi/10.1021/jo00381a024https://doi.org/10.1021/jo00381a024research-articleACS PublicationsRequest reuse permissionsArticle Views304Altmetric-Citations15LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
InP-based Coherent PICs for 100 Gbaud Operation (2019)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text R. Going, S. Wolf, R. Maher, P. Studenkov, V. Lal, H. Tsai, S. Corzine, J. Zhang, B. Behnia, C. Di Giovanni, T. Vallaitis, J. Yan, J. Osenbach, M. Kuntz, T. Frost, H. Mousavi, S. Porto, S. Buggaveeti, H. Hodaei, Z. Wang, X. Xu, P. Evans, J. Rahn, T. Butrie, M. Ziari, D. Welch, and F. Kish, "InP-based Coherent PICs for 100 Gbaud Operation," in OSA Advanced Photonics Congress (AP) 2019 (IPR, Networks, NOMA, SPPCom, PVLED), OSA Technical Digest (Optica Publishing Group, 2019), paper SpM4E.3. Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
High-Pressure Plasma Deposition of a-C:H Films by Dielectric-Barrier Discharge (2003)

The fabrication of a-C:H films from methane has been performed using dielectric-barrier discharges at atmospheric pressure. The effect of combined-feed gas, such as carbon dioxide, carbon monoxide or acetylene on the formation of a-C:H films has been investigated. It has been demonstrated that the addition of carbon monoxide or acetylene into methane leads to a remarkable improvement in the fabrication of a-C:H films. The characterization of carbon film obtained has been conducted using FT-IR, Raman and SEM.
The effect sex chromosomal complement (SCC) on the expression of sodium transporters is dependent on gonadal sex (2009)

The sex chromosome complement (SCC) has been demonstrated to have sex‐steroid‐ independent effects on the male versus female phenotype. To examine the role of the SCC in blood pressure (BP), we created a mouse model by translocation of Sry gene, a testes‐determining gene, onto an autosome in both XX and XY mice. Recently, we showed that ovariectomized mice with the XX SCC had higher angiotensin II (Ang II)‐induced BP as compared to the XY SCC. This effect appeared dominant in mice that were gonadally female at birth. The expression of renal sodium transporters may affect BP. To avoid the activational effect of steroidal hormones, mice were either ovariectomized (OVX) or castrated (CAS) depending upon their gondal sex at birth. All mice were infused with Ang II (A, 800 ng/kg•bw/min, n=5/group) for 7 days. Western blotting revealed that OVX‐XY mice had significantly reduced protein abundance for NaKATPase α‐1subunit and γENaC relative to OVX‐XX mice (band density % XX; for NaKATPase were 63 ± 0.01 vs. 100 ± 0.09 %; for γENaC 72.6 ± 0.03 vs. 100 ± 0.09% ). However, no significant difference due to SCC in these protein levels was observed in the CAS XXSry and XY − Sry male (at birth) mice. Moreover, AQP‐3 was significantly reduced in XY − Sry mice relative to XXSry (62.6 ± 0.12 vs. 100 ± 0.07 %). These data demonstrate that the SCC may influence expression of renal sodium and water transporters independently of activational effects of the gonadal steroids. Increased expression of Na‐K‐ATPase or γENaC could play a role in the increased BP observed in the XX SCC mice. The fact that the XX SCC appeared to increase BP suggests that without the overlay of sex steroids, female mice may be have a greater susceptibility to high BP. This may have particular relevance in menopause. NIH
Zika Virus Dependence on Host Hsp70 Provides a Protective Strategy against Infection and Disease (2019)

The spread of mosquito-borne Zika virus (ZIKV), which causes neurological disorders and microcephaly, highlights the need for countermeasures against sudden viral epidemics. Here, we tested the concept that drugs targeting host proteostasis provide effective antivirals. We show that different cytosolic Hsp70 isoforms are recruited to ZIKV-induced compartments and are required for virus replication at pre- and post-entry steps. Drugs targeting Hsp70 significantly reduce replication of different ZIKV strains in human and mosquito cells, including human neural stem cells and a placental trophoblast cell line, at doses without appreciable toxicity to the host cell. By targeting several ZIKV functions, including entry, establishment of active replication complexes, and capsid assembly, Hsp70 inhibitors are refractory to the emergence of drug-resistant virus. Importantly, these drugs protected mouse models from ZIKV infection, reducing viremia, mortality, and disease symptoms. Hsp70 inhibitors are thus attractive candidates for ZIKV therapeutics with the added benefit of a broad spectrum of action.
Exploring Unstructured Mesh Adaptation for Hybrid Reynolds-Averaged Navier–Stokes/Large Eddy Simulation (2020)

Mesh adaptation methods for the Reynolds-averaged Navier–Stokes (RANS) equations are rapidly maturing and beginning to impact the design of aerospace vehicles. RANS turbulence modeling improvements have slowed and may stagnate. Wall-modeled large eddy simulation (LES) and hybrid RANS/LES (HRLES) may provide an improved modeling capability, but require specialized expertise to construct appropriate meshes and are considered be too expensive for routine practical use. The realization of the CFD Vision 2030 Study includes improving geometry linkage, mesh generation/adaptation, and turbulence modeling/resolving methods for automated management of errors and uncertainties of physics-based, predictive modeling that can set the stage for ensuring a vehicle is in compliance with a regulation or specification (i.e., certification or qualification by analysis). An exploration of mesh adaptation for HRLES is performed to document synergies and challenges between mesh adaptation and HRLES. Vortex breakdown over a delta wing is examined to show the improvement of HRLES over RANS turbulence modeling approaches. A high lift configuration is shown to demonstrate complex geometry capability. Research and development opportunities are identified to advocate for continuing investments that may allow HRLES to enter routine practical use as a tool for aerospace vehicle analysis and design.
Specific cleavage of pre-edited mRNAs in trypanosome mitochondrial extracts. (1992)

RNA editing in Trypanosoma brucei is a posttranscriptional processing event that results in the addition and deletion of uridine residues within several mitochondrial mRNAs. We have examined reactions involving pre-edited precursor RNAs in vitro. In this study, we report specific cleavage of pre-edited cytochrome b (CYb), cytochrome oxidase subunit II (COII), and cytochrome oxidase subunit III (COIII) mRNAs when incubated with T. brucei mitochondrial extracts. The pre-edited CYb RNA was cleaved near the 3'-most uridine addition sites, within the region where editing would be expected to commence. Pre-edited COII mRNA was similarly cleaved adjacent to its small editing domain, while pre-edited COIII RNA was cleaved at multiple sites in the region where uridine addition and deletion occurs in vivo. In contrast, edited versions of CYb, COII, and COIII RNAs were not cleaved within the editing domains. Such differential cleavage of the edited and pre-edited forms of these mRNAs suggests either a direct involvement in RNA editing or involvement in another aspect of mitochondrial gene expression requiring cleavage of pre-edited RNAs.
OC-035 TEST METHODS FOR SURGICAL MESHES - AN INDUSTRY POINT OF VIEW (2022)

Abstract Aim No standardized test methods currently exist for the characterization of the synthetic surgical meshes used in hernia repair and soft tissue reinforcement. While each mesh manufacturer developed their own test methods for comparative analysis, these were never harmonized across industry. A subgroup of the European Association of the Surgical Suture Industry (EASSI) was constituted, the Standardization of Surgical Meshes Working Group (SSM WG) with the objective to reach alignment of surgical meshes test methods. Materials &amp; Methods The SSM WG agreed on the test methods that should be defined to provide a thorough in-vitro-characterization and comparison of surgical meshes. After several interlaboratory iterations, the group established common test protocols which may be performed by people with limited textile background. Extensive inter-laboratory test method verifications were then performed by conducting tests with different specimens by all manufacturers in a consistent manner. Results Common protocols were defined for the following test methods: Linear Tensile Test (Strength and elongation), Burst Test (Strength and displacement), Suture Pull-Out Test (Strength), Pore Size Measurement (Porosity and pore dimensions). 4 meshes were tested by each of the 4 involved manufacturers to assess the protocols validity. Despite methods harmonization, differences could be observed between laboratories. Conclusion The SSM WG recommends following the test protocols described and to maximize the sample size. Due to high sensitivity of specimen and difference in equipment used, only results of tests performed by a single testing center using identical testing features for all tested meshes should be compared.
Uniform Crystal Formation and Electrical Variability Reduction in Hafnium-Oxide-Based Ferroelectric Memory by Thermal Engineering (2021)

In this paper, we achieved excellent variation control, endurance enhancement, and leakage reduction in zirconium (Zr)-doped hafnium oxide (Hf1–xZrxO2) based ferroelectric films by the germination of large ferroelectric grains through extending the duration of rapid thermal annealing without increasing the temperature beyond 700 °C. The pivotal point of this work is to reduce electrical variations in ferroelectric capacitors, which we attribute to the random variation in ferroelectric and dielectric phases. The motivation for this research originally stemmed from Johnson–Mehl–Avrami–Kolmogorov’s theory of nucleation, which predicts that a sufficient time is required for forming any particular phase during phase transformation. Instead of increasing the temperature beyond 700 °C, extending the duration of rapid thermal annealing allows uniform crystal to form, which agrees with the theory. A 10 nm thick Hf1–xZrxO2 film (x = 0.2) has been fabricated. The duration and temperature of rapid thermal annealing (RTA) under a nitrogen (N2) atmosphere at 1 atmospheric pressure (atm) is varied to observe its effect on crystal formation and the electrical properties of the devices. It was observed that very low temperature and short (30 s) duration annealing at 1 atm pressure cannot infuse ferroelectricity in Hf1–xZrxO2. Structural analysis clearly showed the formation of large and uniform ferroelectric domains with negligible impact on surface roughness upon extending the annealing duration up to 180 s. The device-to-device variation in terms of standard deviation of coercive voltage and peak capacitance are reduced from 400 to 17 mV and from 20 to 4 fF/cm2, respectively, by increasing the RTA duration at 700 °C. The devices have also displayed endurance above 1 million cycles and leakage current density below 10 pA/μm2. The simple physics-based process discussed here reduces the thermal budget required for Hf1–xZrxO2, mitigates the randomness in the domain distribution, and infuses deterministic switching. This improvement paves the way for implementing Hf1–xZrxO2-based, deeply scaled ferroelectric devices for memory and steep slope device applications.
MicroRNA Profiling of Human-Induced Pluripotent Stem Cells (2009)

MicroRNAs (miRNAs) are a newly discovered endogenous class of small noncoding RNAs that play important posttranscriptional regulatory roles by targeting mRNAs for cleavage or translational repression. Accumulating evidence now supports the importance of miRNAs for human embryonic stem cell (hESC) self-renewal, pluripotency, and differentiation. However, with respect to induced pluripotent stem cells (iPSC), in which embryonic-like cells are reprogrammed from adult cells using defined factors, the role of miRNAs during reprogramming has not been well-characterized. Determining the miRNAs that are associated with reprogramming should yield significant insight into the specific miRNA expression patterns that are required for pluripotency. To address this lack of knowledge, we use miRNA microarrays to compare the "microRNA-omes" of human iPSCs, hESCs, and fetal fibroblasts. We confirm the presence of a signature group of miRNAs that is up-regulated in both iPSCs and hESCs, such as the miR-302 and 17-92 clusters. We also highlight differences between the two pluripotent cell types, as in expression of the miR-371/372/373 cluster. In addition to histone modifications, promoter methylation, transcription factors, and other regulatory control elements, we believe these miRNA signatures of pluripotent cells likely represent another layer of regulatory control over cell fate decisions, and should prove important for the cellular reprogramming field.