gebhart/patrepeval huggingface dataset:
Rôle de l'ezrine dans le contrôle de la morphogenèse et de l'adhérence des cellules épithéliales (2008)

L’ezrine est une proteine de liaison du cytosquelette d'actine a la membrane plasmique. Sa phosphorylation, en reponse a des signaux provenant des facteurs de croissance ou de la matrice extracellulaire, controle diverses etapes de la morphogenese des cellules epitheliales. Ces signaux extracellulaires sont relayes par les kinases cytoplasmiques de la famille Src et nous avons montre que l’ezrine est une cible de ces kinases. Au cours de ma these, j’ai etudie deux nouveaux partenaires de l'ezrine phosphorylee par les kinases de la famille Src : la kinase Fes et les proteines de la famille Eps8. Nous avons montre que la kinase Fes interagit via son domaine SH2 avec la tyrosine 477 phosphorylee de l’ezrine. Selon la confluence des cellules epitheliales, la forme activee de Fes se localise soit au niveau des adherences focales soit au niveau des jonctions adherentes. La localisation de Fes au niveau des jonctions adherentes depend de son interaction avec l’ezrine. Ce recrutement est necessaire, d’une part pour l’activation de la kinase Fes et d’autre part pour la dissociation des cellules en reponse a une stimulation par l’HGF (Hepatocyte Growth Factor). Cette etude nous a permis d’impliquer l’ezrine et la kinase Fes dans la regulation des jonctions cellulaires en aval de la voie de signalisation induite par l’HGF. Les proteines de la famille Eps8 regulent le remodelage du cytosquelette d'actine en aval des voies de signalisation induites par les facteurs de croissance. Nos observations suggerent un role pour l’interaction ezrine/proteines de la famille Eps8 dans l’organisation des filaments d’actine du cortex cellulaire. Nos resultats indiquent que l’interaction de l’ezrine avec differents partenaires permet l’organisation structurale et fonctionnelle de domaines specifiques de la membrane plasmique des cellules epitheliales.
Effective Capacity in MIMO Channels with Arbitrary Inputs (2016)

Recently, communication systems that are both spectrum and energy efficient have attracted significant attention. Different from the existing research, we investigate the throughput and energy efficiency of a general class of multiple-input and multiple-output systems with arbitrary inputs when they are subject to statistical quality-of-service (QoS) constraints, which are imposed as limits on the delay violation and buffer overflow probabilities. We employ the effective capacity as the performance metric. We obtain the optimal input covariance matrix that maximizes the effective capacity under a short-term average power constraint. Following that, we perform an asymptotic analysis of the effective capacity in the low signal-to-noise ratio and large-scale antenna regimes. In the low signal-to-noise ratio regime analysis, we utilize the first and second derivatives of the effective capacity when the signal-to-noise ratio approaches zero in order to determine the minimum energy-per-bit and also the slope of the effective capacity versus energy-per-bit curve at the minimum energy-per-bit. We observe that the minimum energy-per-bit is independent of the input distribution, whereas the slope depends on the input distribution. In the large-scale antenna analysis, we show that the effective capacity approaches the average transmission rate in the channel with the increasing number of transmit and/or receive antennas. Particularly, the gap between the effective capacity and the average transmission rate in the channel, which is caused by the QoS constraints, is minimized with the number of antennas. In addition, we put forward the non-asymptotic backlog and delay violation bounds by utilizing the effective capacity. Finally, we substantiate our analytical results through numerical illustrations.
Expression of an Aspartate Kinase Homoserine Dehydrogenase Gene Is Subject to Specific Spatial and Temporal Regulation in Vegetative Tissues, Flowers, and Developing Seeds (1997)

Although the regulation of amino acid synthesis has been studied extensively at the biochemical level, it is still not known how genes encoding amino acid biosynthesis enzymes are regulated during plant development. In the present report, we have used the [beta]-glucuronidase (GUS) reporter gene to study the regulation of expression of an Arabidopsis thaliana aspartate kinase-homoserine dehydrogenase (AK/HSD) gene in transgenic tobacco plants. The polypeptide encoded by the AK/HSD gene comprises two linked key enzymes in the biosynthesis of aspartate-family amino acids. AK/HSD-GUS gene expression was highly stimulated in apical and lateral meristems, lateral buds, young leaves, trichomes, vascular and cortical tissues of growing stems, tapetum and other tissues of anthers, pollen grains, various parts of the developing gynoecium, developing seeds, and, in some transgenic plants, also in stem and leaf epidermal trichomes. AK/HSD-GUS gene expression gradually dimished upon maturation of leaves, stems, floral tissues, and embryos. GUS expression was relatively low in roots. During seed development, expression of the AK/HSD gene in the embryo was coordinated with the initiation and onset of storage protein synthesis, whereas in the endosperm it was coordinated with the onset of seed desiccation. Upon germination, AK/HSD-GUS gene expression in the hypocotyl and the cotyledons was significantly affected by light. The expression pattern of the A. thaliana AK/HSD-GUS reporter gene positively correlated with the levels of aspartate-family amino acids and was also very similar to the expression pattern of the endogenous tobacco AK/HSD mRNA as determined by in situ hybridization.
[Contact allergy to eyedrops containing beta-blockers]. (1998)

In six patients (4 women aged 80, 62, 43 and 52 years and 2 men aged 58 and 51 years), who used eyedrops containing beta-blockers for the treatment of glaucoma, allergic contact dermatitis of the eyelids was diagnosed. Three were allergic to metipranolol, 2 to levobunolol and 1 to timolol. In literature, less than 50 cases of hypersensitivity to beta-blockers in eye medication have been reported. There are, however, reasons to assume that sensitization is more frequent: (a) not all patients are referred by the ophthalmologist to the dermatologist; (b) false-negative reactions to patch tests with the commercial preparations and with beta-blockers are not infrequent; (c) they are not routinely tested because beta-blockers are difficult to obtain in pure form; (d) cross-reactions with other beta-blockers are infrequent, and changing to another preparation therefore usually solves the clinical problem. Nevertheless it is advisable to test a battery of beta-blockers (befunolol, levobunolol, metipranolol, timolol) in allergic patients. A test preparation of 2% in water or 3%-10% in petrolatum may be suitable. Control testing in non-exposed individuals is necessary to exclude irritation reactions.
Fully printed, stretchable and wearable bioimpedance sensor on textiles for tomography (2021)

Abstract Electrical impedance tomography (EIT) is a non-invasive, real-time, continuous imaging technique that has multiple applications in health care. EIT is a realizable technique for radiation-free medical imaging ranging from real-time monitoring of bone fracture repair to lung functioning. This work explores the prospect of printing a wearable bioimpedance sensor on textiles for EIT imaging. Screen printing and stencil printing were applied to fabricate the sensor on the textile substrate and the imaging was carried out with the worn sensor on the human body. The first part of this work focuses on developing a flexible textile sensor in the form of a bracelet to obtain cross-sectional images of the forearm that unravel bone features like shape, size and position. However, body parts such as the thorax have added complexities due to their constantly varying perimeter and uneven shape. It is a significant prerequisite for the wearable sensors to apply to dynamic body parts where irregular shape and continuous volume variations occur. The second part of the paper therefore addresses the fabrication and testing of a stretchable textile-based sensor to address such instances of body dynamicity. The proposed stretchable sensor, worn on the thorax, demonstrates the feasibility of imaging such an uneven and dynamic body part. Although the EIT images are inherently attributed to low resolution, this work shows the prospect of wearable imaging applications in health monitoring. Apart from demonstrating the printed sensor for EIT imaging, this paper shows the image rendering quality dependency over the frequency of the signal and the number of electrodes. This work could initiate further research on wearable EIT based health monitoring devices for real-life scenarios.
Superfluidity of Grain Boundaries and Supersolid Behavior (2006)

When two communicating vessels are filled to a different height with liquid, the two levels equilibrate because the liquid can flow. We have looked for such equilibration with solid 4 He. For crystals with no grain boundaries, we see no flow of mass, whereas for crystals containing several grain boundaries, we detect a mass flow. Our results suggest that the transport of mass is due to the superfluidity of grain boundaries.
Frequency-Reconfigurable Antenna for Broadband Airborne Applications (2014)

In this letter, we propose a broadband reconfigurable antenna for aircraft communication that covers the VHF/UHF/L-bands (30-300/300-400/960-1220 MHz). The proposed antenna structure is a modified biconical unipole antenna. The antenna is frequency-reconfigurable using a p-i-n diode switch. In the on state, the antenna operates as a V-type traveling antenna and covers 30-300 and 960-1150 MHz. In the off state, the antenna operates as a unipole antenna and covers 300-400 and 1150-1220 MHz. The maximum power dissipation of the proposed antenna is 18 W. The proposed antenna has VSWR <; 2.5 for all bands. Antenna gain is less than 1.8 dBi at 30-400 MHz and 2.5 dBi at 960-1220 MHz.
Acute cardiovascular and hemodynamic effects of vagus nerve stimulation in conscious hypertensive rats (2018)

Hypertension (HTN) affects over 1 billion people worldwide, with a significant number who are unable to control their blood pressure (BP) with conventional therapies. Recently, novel device-based therapies targeting the autonomic nervous system have been evaluated for treating HTN, including vagus nerve stimulation (VNS). Numerous studies have indicated the beneficial effects of chronic VNS in various models of HTN, however the acute effects of VNS on physiological responses have not been widely investigated. To better understand the acute effects of VNS, this study evaluates cardiovascular and hemodynamic responses from conscious hypertensive rats implanted with VNS stimulators and physiological telemeters for simultaneous monitoring of BP and heart rate (HR) as therapy is applied. We demonstrated that there are no acute changes in mean BP, HR and contractility measures as a result of VNS stimulation. However, there were significant increases in both HR variability and BP variability during VNS, which returned to baseline levels immediately at the cessation of therapy. The small acute changes observed during intermittent VNS could be additive, leading to beneficial chronic changes in BP and HR control, and may help in furthering the understanding of beneficial effects demonstrated in chronic use of VNS therapy.
Broad-bandwidth and ultrafast electromagnetic response of coupled bimetal nanoantennas in few-cycle laser applications (2012)

We explore the field enhancement and temporal response of coupled bi-metal Ag/Au core-shell nanoparticle antennas. The bimetal antennas exhibit ultra-broadband resonances and allow exploiting the local field enhancement for few-cycle laser applications such as elements with an ultrafast response in nanoplasmonic device. We study dimer, trimer and heptamer arrangements and find that the Ag/Au core-shell trimer shows that a very high enhancement factor with an amplitude exceeds 120, but still facilitates an ultrafast response. Such systems may be ideal for the generation of attosecond light pulses based on high harmonic generation by employing nanoplasmonic field enhancement.
Monte Carlo study of the quantum spin-(1/2 Heisenberg antiferromagnet on the square lattice (1989)

A new Monte Carlo sampling technique is used to study the spin-1/2 Heisenberg antiferromagnet on square lattices of up to 256 spins. The energy, specific heat, uniform and staggered susceptibilities, and correlation function are calculated. The correlation length as a function of temperature is deduced from correlation functions and is shown to be consistent with a quantum-renormalized classical approximation. Using the coupling constant provided by Raman scattering experiments, good agreement is found between the calculated and experimental correlation lengths in ${\mathrm{La}}_{2}$${\mathrm{CuO}}_{4}$.
Direct Measurement of the Thermodynamic Parameters of Amyloid Formation by Isothermal Titration Calorimetry (2004)

Amyloid fibril deposition is associated with over 20 degenerative diseases, including Alzheimer's, Parkinson's, and prion diseases. Although research over the last few years has revealed the morphology and structural features of the amyloid form, knowledge about the thermodynamics of amyloid formation is limited. Here, we report for the first time a direct thermodynamic study of amyloid formation using isothermal titration calorimetry. Beta(2)-microglobulin, a protein responsible for dialysis-related amyloidosis, was used for extending amyloid fibrils in a seed-controlled reaction in the cell of the calorimeter. We investigated the enthalpy and heat capacity changes of the reaction, where the monomeric, acid-denatured molecules adopt an ordered, cross-beta-sheet structure in the rigid amyloid fibrils. Despite the dramatic difference in morphology, beta(2)-microglobulin exhibited a similar heat capacity change upon amyloid formation to that of the folding to the native globular state, whereas the enthalpy change of the reaction proved to be markedly lower. In comparison with the native state, the results outline the important structural features of the amyloid fibrils: a similar extent of surface burial even with the supramolecular architecture of amyloid fibrils, a lower level of internal packing, and the possible presence of unfavorable side chain contributions.
Sperm-Associated Antigen 6 (SPAG6) Deficiency and Defects in Ciliogenesis and Cilia Function: Polarity, Density, and Beat (2020)

SPAG6, an axoneme central apparatus protein, is essential for function of ependymal cell cilia and sperm flagella. A significant number of Spag6-deficient mice die with hydrocephalus, and surviving males are sterile because of sperm motility defects. In further exploring the ciliary dysfunction in Spag6-null mice, we discovered that cilia beat frequency was significantly reduced in tracheal epithelial cells, and that the beat was not synchronized. There was also a significant reduction in cilia density in both brain ependymal and trachea epithelial cells, and cilia arrays were disorganized. The orientation of basal feet, which determines the direction of axoneme orientation, was apparently random in Spag6-deficient mice, and there were reduced numbers of basal feet, consistent with reduced cilia density. The polarized epithelial cell morphology and distribution of intracellular mucin, α-tubulin, and the planar cell polarity protein, Vangl2, were lost in Spag6-deficient tracheal epithelial cells. Polarized epithelial cell morphology and polarized distribution of α-tubulin in tracheal epithelial cells was observed in one-week old wild-type mice, but not in the Spag6-deficient mice of the same age. Thus, the cilia and polarity defects appear prior to 7 days post-partum. These findings suggest that SPAG6 not only regulates cilia/flagellar motility, but that in its absence, ciliogenesis, axoneme orientation, and tracheal epithelial cell polarity are altered.
PCI Express® 6.0 Specification at 64.0 GT/s with PAM-4 signaling: a low latency, high bandwidth, high reliability and cost-effective interconnect (2020)

PCI Express® (PCIe®) specification doubles the data rate every generation in a backwards compatible manner every three years. With PCIe 6.0 specification at 64.0 GT/s, we will be adopting PAM-4 signaling to ensure the channel reach remains the same as PCIe 5.0 specification at 32.0 GT/s. PAM-4 requires a Forward Error Correction (FEC) mechanism due to the high BER. However, since PCIe technology is a Load-Store architecture, the latency adder due to FEC must be less than 10ns and ideally 0. We present the new flit-based approach with a light-weight low-latency FEC coupled with a low-latency link level retry mechanism. We demonstrate that PCIe 6.0 architecture improves the bandwidth efficiency in the range of 0.95 to 1.4, resulting in a net bandwidth increase of 1.9X to 2.8X, depending on the payload size of the packets, in spite of the FEC and CRC overheads. The latency also decreases in most cases. The reliability (FIT) of our approach is demonstrated to less than 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-9</sup> . We also present a new power savings strategy that results in power consumption proportional to bandwidth usage without impacting the traffic flow.
Molecular Level-based Analysis of Organosolv Wastewater (2017)

Background: Identification of monomeric and oligomeric organic compounds in Organosolv process wastewater is of growing importance in the context of isolating high value chemicals and producing biofuels. There were many studies devoted to identification of such compounds in precipitated Organosolv lignin, all of them using demanding, mostly hyphenated techniques such as HPLC-MS/MS and two dimensional NMR spectroscopy. However, the resulting Organosolv wastewater has not been studied as intensively in this respect, even though the application of these techniques to identify the components of dried/lyophilized Organosolv wastewater would be straightforward. Objective: The objective was to identify lignin-based breakdown products in Organosolv wastewater resulting from pulping of beech with aqueous ethanol solutions. Method: Low resolution GC/MS analysis in combination with derivatization reactions using nonlabeled and isotopically labeled derivatization agents and retention index information (where available) were used. Results: The application of isotopically labeled derivatization agents such as BSTFA-d9 (N,Obis( trimethylsilyl)trifluoracetamide-d9) turned out especially useful in structural identification work. In addition to monomers such as abundant syringaldehyde and vanillin, a multitude of dimeric products of the guaiacyl(syringyl) glycerol-β-guaiacyl(syringyl) ether-type, phenylcoumaran type and lignan-type could be detected, the most abundant surrogate being syringaresinol. Syringyl-type surrogates were more abundant compared to guaiacyl-type surrogates. Formation pathways of the monomeric breakdown products such as syringaldehyde and vanillin were proposed on the basis of the solvolytic cleavage of β-O-4 aryl ether linkages. Conclusion: Solvolytic cleavage of β(α)-O-4, β-5 and β-β linkages within the macromolecular lignin network under Organosolv process conditions results in dimeric products. Further activities should be focused on the use of high resolution MS and the synthesis of authentic standards. Keywords: Aryl ether bonds, derivatization, GC/MS analysis, lignin, NMR spectroscopy, organosolv wastewater.
Metabolic Activation of Polycyclic Aromatic Hydrocarbons and Aryl and Heterocyclic Amines by Human Cytochromes P450 2A13 and 2A6 (2013)

Human cytochrome P450 (P450) 2A13 was found to interact with several polycyclic aromatic hydrocarbons (PAHs) to produce Type I binding spectra, including acenaphthene, acenaphthylene, benzo[c]phenanthrene, fluoranthene, fluoranthene-2,3-diol, and 1-nitropyrene. P450 2A6 also interacted with acenaphthene and acenaphthylene, but not with fluoranthene, fluoranthene-2,3-diol, or 1-nitropyrene. P450 1B1 is well-known to oxidize many carcinogenic PAHs, and we found that several PAHs (i.e., 7,12-dimethylbenz[a]anthracene, 7,12-dimethylbenz[a]anthracene-5,6-diol, benzo[c]phenanthrene, fluoranthene, fluoranthene-2,3-diol, 5-methylchrysene, benz[a]pyrene-4,5-diol, benzo[a]pyrene-7,8-diol, 1-nitropyrene, 2-aminoanthracene, 2-aminofluorene, and 2-acetylaminofluorene) interacted with P450 1B1, producing Reverse Type I binding spectra. Metabolic activation of PAHs and aryl- and heterocyclic amines to genotoxic products was examined in Salmonella typhimurium NM2009, and we found that P450 2A13 and 2A6 (as well as P450 1B1) were able to activate several of these procarcinogens. The former two enzymes were particularly active in catalyzing 2-aminofluorene and 2-aminoanthracene activation, and molecular docking simulations supported the results with these procarcinogens, in terms of binding in the active sites of P450 2A13 and 2A6. These results suggest that P450 2A enzymes, as well as P450 Family 1 enzymes including P450 1B1, are major enzymes involved in activating PAHs and aryl- and heterocyclic amines, as well as tobacco-related nitrosamines.
Effects of weight loss or exercise on muscle quality in obese older women (2012)

A key component in lower extremity physical function in older obese women is leg muscle quality (MQ), defined as: a) strength per muscle quantity or b) the degree of adipose intermuscular adipose tissue (IMAT) within the muscle. This pilot study assessed the relative impact of a weight loss diet (WL‐D) or exercise (EX) on MQ. Sedentary obese (BMI ≥ 30–35 kg/m 2 ) women (N = 30) were randomized to a WL‐D or EX intervention for 16 weeks. Thigh subcutaneous adipose tissue (SAT), IMAT, and muscle (MUSC) was quantified by MRI. Strength was assessed via maximal isometric (IM) and isokinetic (at 60 deg/sec, IK60) contractions using an isokinetic dynamometer and calculated as Nm/(m 3 ). WL‐D (n=8, 65.6±8.0 y) lost 2.4±3.9 kg of body weight (p=0.12) and EX (n=11, 63.7±4.5 y) lost did not change (0.06±1.8 kg; p=0.91). Changes in upper leg composition or MQ measures did not significantly differ between groups; however; a) SAT decreased 3.5% in WL‐D (p=0.11) and 3% in EX (p=0.01), b) IMAT increased 7% in WL‐D (p=0.04) but only 2.7% in EX (p=0.27), c) MUSC increased 3.6% in WL‐D (p=0.34) and 4.5% in EX (p=0.06), d) EX increased MQ‐IM 7.3% (p=0.17) and MQ‐IK60 1.6% (p=0.80) whereas WL‐D increased MQ‐IM 0.89% (p=0.79) and decreased MQ‐IK60 −8.2% (p=0.12). Weight loss and exercise may have differential effects on thigh composition and MQ in older obese women. Supported by NIH‐HL090455
Binary and Ternary Metal Complexes of Uridine and Thermodynamic Quantities Associated with the Interaction of Uridine with Bivalent Metal ions in Solution (1987)

Abstract Formation of binary and ternary complexes of Cu(II), Ni(II), Zn(II), Co(II), Mn(II), Mg(II), Ca(II), metal ions with uridine as a primary ligand and 2,2′-bipyridyl, 1,10-phenanthroline and 5-sulphosalicylic acid as secondary ligands was studied by a potentiometric technique at 15°, 25°, 35° and 45°C and 0.10 M (KNO3) ionic strength. The ternary complex formation was found to take place in a stepwise manner. The stability constants of these binary and ternary systems were calculated and the Δ log K values obtained were found to be negative. The lower stability of 1:2 complexes of uridine and those of the ternary systems compared to the corresponding binary systems is in accord with statistical considerations. Thermodynamic parameters are reported for all the systems studied. Key Words: Uridinestability constantsdivalent metals N-heterocyclescomplexes