gebhart/patrepeval huggingface dataset:
Purification and characterization of recombinant human farnesyl diphosphate synthase expressed in <i>Escherichia coli</i> (1991)

We previously reported the isolation of a partial-length human fetal-liver cDNA encoding farnesyl diphosphate (FPP) synthase (EC 2.5.1.10) and the expression of an active FPP synthase fusion protein in Escherichia coli. The expressed human FPP synthase fusion protein has now been purified to apparent homogeneity by using two chromatographic steps. The purification scheme allowed the preparation of 1.8 mg of homogeneous protein from 149 mg of crude extract in a 64% yield with a 52-fold enrichment. A single band with a subunit molecular mass of 39 kDa was observed by Coomassie Blue staining after SDS/PAGE. A molecular mass of 78-80 kDa was calculated for the native form of the fusion protein by h.p.l.c. on a SEC-250 column, suggesting that the active fusion protein is a dimer. The purified fusion protein has FPP synthase condensation activities in the presence of both substrates, isopentenyl diphosphate and geranyl diphosphate. Enzyme activity was inhibited by a bisubstrate analogue of isopentenyl diphosphate and dimethylallyl diphosphate, and a small amount of higher prenyltransferase was observed. Michaelis constants for isopentenyl diphosphate and geranyl diphosphate were 0.55 and 0.43 microM respectively, and Vmax for synthesis of farnesyl diphosphate from these substrates was 1.08 mumol/min per mg. These results suggest that the structure and catalytic properties of the expressed FPP synthase fusion protein are virtually identical with those of the native human liver enzyme.
Pressure-sensitive pen interactions (2008)

Pen-based computers bring the promise of tapping into people’s expressiveness with pen and paper and producing a platform that feels familiar while providing new functionalities only possible within an electronic medium. To this day, pen computers’ success is marginal because their interfaces mainly replicate keyboard and mouse ones. Maximizing the potential of pen computers requires redesigning their interfaces so that they are sensitive to the pen’s input modalities and expressiveness. In particular, pressure is an important and expressive, yet underutilized, pen input modality. This dissertation advances our knowledge about pressure-aware, pen-based interactions and how people use these techniques. We systematically explore their design by first investigating how pressure can affect pen interactions. We propose novel techniques that take advantage of the pressure modality of a pen to control, link, and annotate digital video. We then study people’s performance using pressure to navigate through a set of elements and find that they can discriminate a minimum of six different pressure regions. We introduce the concept of Pressure Widgets and suggest visual and interaction properties for their design. We later explore pressure’s use to enhance the adjustment of continuous parameters and propose Zliding, a technique in which users vary pressure to adjust the scale of the parameter space, while sliding their pen to perform parameter manipulations. We study Zliding and find it a viable technique, which is capable of enabling arbitrarily precise parameter adjustments. We finally present a novel interaction technique defined by the concurrent variation in pressure applied while dragging a pen. We study these pressure marks and find that they are a compact, orientation-independent, full interaction phrase that can be 30% faster than a stateof-the-art selection-action interaction phrase. This dissertation also makes a number of key contributions throughout the design and study of novel interaction techniques: -It identifies important design issues for the development of pressure-sensitive, pen operated widgets and interactions, -It provides design guidelines for interaction techniques and interface elements utilizing pressure-enabled input devices, -It presents empirical data on people’s ability to control pressure, and -It charts a visual design space of pressure-sensitive, pen-based interactions.
Deriving minimal models for resource utilization (2013)

We show how compact Resource Utilization Models (RUMs) can be extracted from concrete overly-detailed models of systems or sub-systems in order to model energy-aware software. Using the Counterexample-Guided Abstraction Refinement (CEGAR) approach, along with model-checking tools, abstract models can be generated that help establish key properties relating to energy consumption. This approach is illustrated by the concrete example of a network manager sub-system. This work is part of an overall design methodology for energy-aware software.
Indocyanine Green Angiography in the Surgical Evaluation of Partial Thickness Burn Wounds: A Prospective Study (2020)

Mulay, Sagar R. MD; Klein, Gabriel M. MD; Grosser, Sarah BS, MA; Chiu, Katherine MPH; Marquez, Jocellie MD, MBA; Sandoval, Steven MD; Dagum, Alexander B. MD, FACS Author Information
Ionic Hydrogels Based Wearable Sensors to Monitor the Solar Radiation Dose for Vitamin D Production and Sunburn Prevention (2021)

Wearable solar radiation sensors based on ionic hydrogels are facilely prepared to simultaneously monitor the radiation dose for the production of vitamin D and the prevention of sunburn. Tetramethylethylenediamine (TEMED) is neutralized with acrylic acid (AA) to obtain tetramethylethylenediamine acrylate (TEMEDA), which is further polymerized with acrylamide by a free radical reaction. By simply adding MB or NR during the polymerization, the final obtained ionic hydrogels can indicate solar radiation. Due to the extent of discoloration, the discoloration speed of MB and NR is correlated to the radiation dose. This wearable sensor can indicate the solar radiation dose required by the human body to synthesize vitamin D through the discoloration of the ionized hydrogel of MB, whereas those with NR are able to illustrate the threshold of radiation dose that causes potential skin hurt. Therefore, the benefit and drawback of solar radiation can be well balanced by optimizing the exposure time to solar irradiation. In addition, polyurethane cross-linked with a thermoresponsive coating is used as band for this wearable sensor. Due to the hydrophilicity below its transition temperature, the cross-linked band possesses the easy cleaning capability of stains after the daily wear. Such type of wearable sensor can be broadly used for monitoring the solar radiation, especially in outdoor activities.
Abstract CN01-01: Genetic and epigenetic changes in prostate cancer as targets for prevention (2008)

Abstract CN01-01 Prostate cancer cells are known to carry a variety of genetic defects, including gene translocations (eg. TMPRSS-ERG1), amplifications (eg. AR), mutations, and deletions. These changes are thought to endow the prostate cancer cells with new capabilities for dysregulated proliferation, inappropriate survival, tissue invasion and destruction, immune system evasion, and metastasis. In addition, cancer cells also carry epigenetic defects, manifest as changes in CpG dinucleotide methylation patterns and in chromatin structure and organization (eg. at GSTP1 and many others), which are equivalent to genetic changes in promoting malignant phenotypes. During the pathogenesis of prostate cancer, the somatic epigenetic alterations tend appear earlier during cancer development than genetic changes, as well as more commonly and consistently. For this presentation, new data concerning the mechanism(s) by which androgen action might promote translocations at AR target genes, and by which inflammatory processes might trigger CpG island hypermethylation, in prostate cells will be presented. These new insights into the formation of somatic genetic and epigenetic changes will provide new molecular biomarkers to aid in prostate cancer detection and diagnosis, and may lead to new opportunities for prostate cancer prevention and treatment. Citation Information: Cancer Prev Res 2008;1(7 Suppl):CN01-01.
The application of carbon nanotube electron sources to the electron microscope (2008)

The promising field emission properties of carbon nanotubes, or CNTs, have resulted in them being identified as desirable sources for electron microscopes and other electron beam equipment. A new process to grow single CNTs aligned to the electron-optical axis inside electron source modules has been developed. The process involves putting the entire source-suppressor module inside a plasma-enhanced chemical vapour deposition reaction chamber. This is a process which can be scaled up to mass production. The resultant CNT electron sources were inserted into an electron microscope for imaging. Though current stability was found to be comparable to the tungsten cold-field emitter (with a maximum-minimum variation of 3-7% of the mean current over one hour), the reduced brightness was found to be an order of magnitude greater than a typical Schottky source (at 3&times;10<sup>9</sup> Acm<sup>2</sup>sr<sup>-1</sup>) with a kinetic energy spread of 0.28 eV. Imaging with a CNT source has produced a marked improvement in resolution when compared to a Schottky source using the same electron-optics. The properties measured show that the CNT source compares favourably with and in some cases improves upon other sources available today. In particular, the CNT source would be of most benefit to low-voltage, high-resolution microscopy.
Some aspects of the chemistry of diazoalkanes (1985)

The discussion of the research reported in this thesis is prefaced by a review on diazoalkane chemistry.&#13;\nThe initial object of this work was to continue and expand upon the work previously reported on thiophenium ylids.&#13;\n3-Substituted thiophenium ylids had not previously been synthesized. Attempts to synthesize these compounds using reported conditions met with limited success, only low yields of the desired ylids were obtained.&#13;\nThe use of rhodium-(II)-hexanoate as a catalyst in the ylid forming reactions was found to be more effective in certain circumstances.&#13;\nThe thermal rearrangement of thiophenium ylids to thiophen-2-malonates was found to proceed via a 2(H)-thiopyran intermediate.&#13;\nNmr studies of the substituted ylids showed non equivalent ester signals with a barrier to their exchange of 47-60 kJ mole-1. Two exchange processes were found to be taking place, inversion at sulphur and rotation about the ylid bond, with inversion at sulphur having the lower barrier. The rotation about the ylidcarbon-carbonylcarbon bond was also hindered, this having a barrier of 38 kJ mole-1.&#13;\nReaction of dimethyl diazomalonate with aliphatic amines resulted in the formation of 1,2,3-triazoles in good yield. However, the reaction of other diazoesters with the amines did not give the corresponding triazoles&#13;\nAn attempt was made to synthesize the phytoalexin Wyerone using the rearrangement of an a-diazomethylthiophen to generate the cis-ene-yne side chain. The intermediate diazoalkane could not be prepared in sufficient yield to allow further transformation. The rhodium-(II)-acetate catalysed reaction of a closely related model compound resulted in the formation of the corresponding ketone.
Study of network cooperation system between three different types of robots (2020)

In this system, three robots with different functions are networked, and the robots can cooperate by sharing the information obtained by each robot. This system uses RT middleware and robot service network protocols(RSNP) as common basic technologies. By using these technologies, robots can be easily networked. We have conducted field experiments using three different robots and one scenario for cooperation. The three different robots are two types of communication robots and mobile robot. In this case, the data obtained by each robot is transmitted to the server using RSNP, a system for sharing data with other robots was developed, and was verified the demonstration experiment at international robot exhibition 2019.
Demonstration of a compact 100 Hz, 01 J, diode-pumped picosecond laser (2011)

We have demonstrated an all-diode-pumped Yb:YAG chirped pulse amplification laser that produces 100 mJ pulses of 5 ps duration at 100 Hz repetition rate. The compact laser system combines a room-temperature Yb:YAG regenerative amplifier for increased bandwidth and a cryogenically cooled Yb:YAG four-pass amplifier for improved heat dissipation and increased efficiency. The optical efficiency of this amplifier is higher than that of other diode-pumped systems of comparable energy.
ChemInform Abstract: CONFORMATIONAL CHARACTERISTICS OF RIGID CYCLIC NUCLEOTIDES. 3. THE SOLUTION CONFORMATION OF β‐LYXONUCLEOSIDE CYCLIC 2′,5′‐ AND 3′,5′‐MONOPHOSPHATES AND OF α‐ARABINONUCLEOSIDE CYCLIC 2′,5′‐MONOPHOSPHATES. IMPLICATIONS FOR EVALUATION OF THE SOLUTION PROPERTIES OF NUCLEOSIDE ANALOGS (1981)

Abstract Detaillierte NMR‐spektroskopische Untersuchungen werden zur Konformationsanalyse der Titelverbindungen (I), (II) und (III) durchgeführt.
Faculty Opinions recommendation of How we diagnose and treat systemic mastocytosis in adults. (2019)

Rapid advances in the understanding of the molecular biology, data from translational and clinical trials, and retrospective analyses has influenced the diagnosis and treatment of systemic mastocytosis (SM). Many options have existed for the symptomatic management of SM patients, but recent evolution in regards to the molecular underpinnings of this disease and our ability to distinguish clonal mastocytosis from mast cell activation syndrome has changed our treatment paradigm and opened new opportunities for understanding genetic risk, transformation to mast cell leukaemia, and treatment choices. Key to this change has been the discovery of the KIT mutation and the use of next generation sequencing to evaluate for co-existing molecular mutations that may define the disease course. Careful diagnosis, judicious symptom management and close surveillance of those who may have yet undiagnosed disease is paramount in providing optimal management. In this article, we review the diagnosis and provide a paradigm for the management of SM patients.© 2017 John Wiley & Sons Ltd. PMID: 29048112
Designing multi-purpose devices to enhance users’ perception of haptics (2022)

Abstract While Virtual Reality has slowly become a common sight, haptics is still struggling to appeal to the general public. We argue that one of the possible reasons is that while VR is designed to be as easily adaptable as possible to many different contexts, haptics is often designed to fulfil a specific purpose and fails to present itself as a tool that can be exploited by designers. To test our hypothesis, we created a VR game where a wrist exoskeleton was used to interact with the environment. The game was composed of multiple levels, some of which also featured a metaphorical interaction through the same haptic device, and was tested by expert haptics scholars during a conference. Preliminary results suggest that by showing multiple potential usages of an exoskeleton, it was possible to enhance users’ interest towards the haptic device and the game.
Pseudomonas taeanensis sp. nov., isolated from a crude oil-contaminated seashore (2010)

A novel Gram-negative, aerobic, motile, short rod-shaped bacterium, designated MS-3(T), was isolated from a crude oil-contaminated seashore in Taean, Korea. Strain MS-3(T) grew at 4-30 °C, at pH 6.0-9.5 and with 0-5 % NaCl and was oxidase- and catalase-positive. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MS-3(T) was most similar to Pseudomonas marincola KMM 3042(T) (97.9 % 16S rRNA gene sequence similarity), P. cuatrocienegasensis 1N(T) (97.8 %), P. borbori R-20821(T) (97.3 %) and P. lundensis ATCC 49968(T) (97.1 %). Relatively low levels of DNA-DNA relatedness were found between strain MS-3(T) and P. cuatrocienegasensis LMG 24676(T) (57.2 %), P. borbori LMG 23199(T) (39.7 %), P. marincola KMM 3042(T) (32.2 %) and P. lundensis KACC 10832(T) (32.1 %), which support the classification of strain MS-3(T) within a novel species of the genus Pseudomonas. The G+C content of the genomic DNA of strain MS-3(T) was 57.6&emsp14;mol% and the major isoprenoid quinone was Q-9. Strain MS-3(T) contained summed feature 3 (iso-C(15 : 0) 2-OH and/or C(16 : 1)ω7c; 38.0 %), C(16 : 0) (24.4 %), C(18 : 1)ω7c (12.8 %), C(12 : 0) (9.6 %) and C(10 : 0) 3-OH (4.9 %) as the major cellular fatty acids. On the basis of the phenotypic, genotypic and phylogenetic data, strain MS-3(T) represents a novel species of the genus Pseudomonas, for which the name Pseudomonas taeanensis sp. nov. is proposed. The type strain is MS-3(T) (=KCTC 22612(T) =KACC 14032(T) =JCM 16046(T) =NBRL 105641(T)).
Technetium(III) complexes with the tetradentate "umbrella" ligand tris(o-mercaptophenyl)phosphinate: x-ray structural characterization of Tc(P(o-C6H4S)3)(CNC3H7) and Tc(P(o-C6H4S)3)(CNC3H7)2 (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTechnetium(III) complexes with the tetradentate "umbrella" ligand tris(o-mercaptophenyl)phosphinate: x-ray structural characterization of Tc(P(o-C6H4S)3)(CNC3H7) and Tc(P(o-C6H4S)3)(CNC3H7)2Nadine De Vries, Jessica Cook, Alun G. Jones, and Alan DavisonCite this: Inorg. Chem. 1991, 30, 12, 2662–2665Publication Date (Print):June 1, 1991Publication History Published online1 May 2002Published inissue 1 June 1991https://pubs.acs.org/doi/10.1021/ic00012a019https://doi.org/10.1021/ic00012a019research-articleACS PublicationsRequest reuse permissionsArticle Views103Altmetric-Citations34LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Evaluation of guidewire path reproducibility (2008)

The number of minimally invasive vascular interventions is increasing. In these interventions, a variety of devices are directed to and placed at the site of intervention. The device used in almost all of these interventions is the guidewire, acting as a monorail for all devices which are delivered to the intervention site. However, even with the guidewire in place, clinicians still experience difficulties during the interventions. As a first step toward understanding these difficulties and facilitating guidewire and device guidance, we have investigated the reproducibility of the final paths of the guidewire in vessel phantom models on different factors: user, materials and geometry. Three vessel phantoms (vessel diameters approximately 4 mm) were constructed having tortuousity similar to the internal carotid artery from silicon tubing and encased in Sylgard elastomer. Several trained users repeatedly passed two guidewires of different flexibility through the phantoms under pulsatile flow conditions. After the guidewire had been placed, rotational c-arm image sequences were acquired (9 in. II mode, 0.185 mm pixel size), and the phantom and guidewire were reconstructed (512(3), 0.288 mm voxel size). The reconstructed volumes were aligned. The centerlines of the guidewire and the phantom vessel were then determined using region-growing techniques. Guidewire paths appear similar across users but not across materials. The average root mean square difference of the repeated placement was 0.17 +/- 0.02 mm (plastic-coated guidewire), 0.73 +/- 0.55 mm (steel guidewire) and 1.15 +/- 0.65 mm (steel versus plastic-coated). For a given guidewire, these results indicate that the guidewire path is relatively reproducible in shape and position.
Direct observation of the reversible redox couple trisilver(2+) (Ag32+) .dblarw. trisilver (Ag30) in silver zeolite A by Fourier transform far-infrared - infrared spectroscopy (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDirect observation of the reversible redox couple trisilver(2+) (Ag32+) .dblarw. trisilver (Ag30) in silver zeolite A by Fourier transform far-infrared - infrared spectroscopyMark D. Baker, Geoffrey A. Ozin, and John GodberCite this: J. Phys. Chem. 1984, 88, 21, 4902–4904Publication Date (Print):October 1, 1984Publication History Published online1 May 2002Published inissue 1 October 1984https://pubs.acs.org/doi/10.1021/j150665a020https://doi.org/10.1021/j150665a020research-articleACS PublicationsRequest reuse permissionsArticle Views129Altmetric-Citations40LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts