gebhart/patrepeval huggingface dataset:
Structure formation during drying of coating layer of magnetic particulate containing solvent mixture. (1995)

It is important to understand the structure formation during drying of coating layers of magnetic particulate from the viewpoint of design of dryers and better quality of magnetic recording media. Drying characteristics of coating layers containing cyclohexanone and toluene were measured, and the change of intensity of reflected light at the layer surface was determined during drying. Then, surface and inside characteristics of dry layers were evaluated by gloss measurement and nitrogen adsorption. If the initial mass fraction of toluene was less than 50 wt% in the solvent mixture, toluene shortened the drying time and did not influence the gloss and the pore volume of dry layers. The influence of drying conditions on surface and inside characteristics of dry layers was qualitatively explained based on the model previously proposed by the authors.
Comparison of methodologies for quantitative analysis of [18F]FLT PET in tumor xenografts in mice (2008)

603 Objectives: Although 39-deoxy-39-18F-fluorothymidine (FLT) PET studies have been performed in various tumor models in mice, only the semi-quantitative approaches were used. The aim of this study was to compare the methodologies for the quantitative FLT PET studies in tumor xenografts in mice. Methods: We investigated the kinetic models of FLT in A431 (n=7) and LLC (n=10) tumor xenografts. PET scans were acquired for 2 hours. Goodness-of-fit for tissue time activity curves (CT) using 3-compartment models with reversible (3C5P) and irreversible phosphorylation (3C4P) was compared. The effects of incorporating blood volume fraction (Vb) in the model were also tested. Several macro parameters associated with FLT metabolism, such as net influx rate (KFLT), total distribution volume (DVtot), and DV of FLT phosphorylated nucleotides (DVm), were explored. Patlak graphical analysis (PGA) was performed and SUV was also calculated. These parameters were compared to the results of Ki-67 immunohistochemical analysis and thymidine kinase assay. Results: 3C5P model was best to describe the CT and the curve fitting was more accurate when Vb term was included. KFLT was significantly underestimated with 3C4P model or PGA. The A431 and LLC tumors showed markedly different levels of the PET parameters (k3, k3/k4, KFLT, DVm, DVtot, and SUV), which agree with the ex vivo analysis results. KFLT-3C5P, that showed smallest variability among the macro parameters, strongly correlated with k3 but was not dominated by K1. Conclusions: The 3-compartment model with reversible phosphorylation and variable Vb was best to describe the FLT kinetics in mice. Kinetic modeling analysis with this model provided reliable kinetic parameters to measure the tumor proliferation.
Recovery of lysozyme from hen egg white by selective magnetic cake filtration (2011)

Abstract A new concept for the improvement of the downstream processing and purification is the so‐called magnetic separation. By using surface functionalized magnetic substrate particles, which selectively adsorb the target product, it can be directly separated out of a crude bioprocess stream. These methods are already used for analytical purposes, where only small amounts of functionalized particles are necessary. To apply the same concept at a larger scale, effective and economical procedures have to be provided. First, suitable process equipment has to be developed. Second, the magnetic particles have to be manufactured with a stable surface functionalization and long‐term stability for their reuse. Up to now mainly high‐gradient magnetic separation filter devices are applied for selective magnetic separation. They consist of a magnetic matrix in which the magnetic particles are trapped. In this work, a new magnetic filter is introduced that overcomes the capacity limitations of the current high‐gradient magnetic separation technology. The principle is demonstrated by selective recovery of lysozyme from hen egg white. Prior to the separation experiments magnetic beads with a strong acid cation‐exchange surface functionalization are synthesized. The separation procedure is implemented in only one unit operation. With the implementation of the displacement elution sequence lysozyme can be separated out of a hen egg white solution with a purification factor of PF =36 and a purity P =0.83.
Figure S2 from Monoclonal Antibody Targeting Sialyl-di-Lewis&lt;sup&gt;a&lt;/sup&gt;–Containing Internalizing and Noninternalizing Glycoproteins with Cancer Immunotherapy Development Potential (2023)

&lt;p&gt;FG129 and CH129 target their glyco-epitope in vitro with nanomolar functional affinity&lt;/p&gt;
Compound heterozygote mutations in <i>SPG7</i> in a family with adult-onset primary lateral sclerosis (2016)

Compound heterozygote mutations in SPG7 are associated with adult-onset PLS, extending the spectrum of SPG7-linked neurologic diseases. Patients with the PLS phenotype should have genetic testing for paraplegin, especially when the condition is familial.
Coronary Sinus Intervention in Cardiac Surgery (2000)

Topics include basic considerations and techniques, venous drainage of the myocardium, structure and function of the cardiac lymphatic system, coronary sinus interventions in experimental research, and more.
NF1 regulation of RAS/ERK signaling is required for appropriate granule neuron progenitor expansion and migration in cerebellar development (2014)

Cerebellar development is regulated by a coordinated spatiotemporal interplay between granule neuron progenitors (GNPs), Purkinje neurons, and glia. Abnormal development can trigger motor deficits, and more recent data indicate important roles in aspects of memory, behavior, and autism spectrum disorders (ASDs). Germline mutation in the NF1 tumor suppressor gene underlies Neurofibromatosis type 1, a complex disease that enhances susceptibility to certain cancers and neurological disorders, including intellectual deficits and ASD. The NF1 gene encodes for neurofibromin, a RAS GTPase-activating protein, and thus negatively regulates the RAS signaling pathway. Here, using mouse models to direct conditional NF1 ablation in either embryonic cerebellar progenitors or neonatal GNPs, we show that neurofibromin is required for appropriate development of cerebellar folia layering and structure. Remarkably, neonatal administration of inhibitors of the ERK pathway reversed the morphological defects. Thus, our findings establish a critical cell-autonomous role for the NF1-RAS-ERK pathway in the appropriate regulation of cerebellar development and provide a basis for using neonatal ERK inhibitor-based therapies to treat NF1-induced cerebellar disorders.
Test results of pre-production prototype distributed ion pump design for the PEP-II asymmetric B-Factory collider (2002)

We have built and tested a plate-type pre-production prototype distributed ion pump (DIP) for the PEP-II B-Factory High Energy Ring (HER). The design has been simplified from an earlier design to use less materials and to reduce overall costs. Penning cell hole sizes of 15, 18, and 21 mm have been tested in a uniform magnetic field of 0.18 T to optimize pumping speed. The resulting final DIP design consisting of a 7-plate, 15 mm basic cell size anode was predicted to give the best pumping speed results for the actual varying magnetic field of the HER dipole. A description of the final optimized DIP design will be presented along with the test results of the pumping speed measurements.
Bile Salts and N-Acetyl-D-Glucosamine induce transcription of the <i>vieSAB</i> Operon in <i>Vibrio cholerae</i> El Tor (2022)

Abstract The vieSAB operon regulates motility, biofilm formation and cholera toxin production in the classical biotype of V. cholerae . Classical biotype mutants lacking the cyclic-di-GMP phosphodiesterase and response regulator vieA or its cognate sensor kinase vieS fail to effectively colonize the infant mouse small intestine, are less motile than the wild-type strain, and are hyper-biofilm formers. However, deletion of vieA or vieS in the currently-circulating El Tor biotype does not have a demonstrable effect on these phenotypes. To begin to understand the role of vieSAB in the El Tor biotype, we studied its regulation and the effect of vieA expression on bacterial physiology, separating the functional roles of VieA’s enzymatic and DNA-binding activities. We identify bile salts and N-acetylglucosamine, which can be found at high concentration in the small intestine, as stimulating expression of vieSAB during in vitro growth. We show that, in contrast to many response regulators, VieA does not regulate its own expression. Together, these data indicate that V. cholerae upregulates vieSAB in response to small-intestinal signals, allowing VieA to alter the physiology of the cell through its transcriptional regulation and cyclic-di-GMP signaling activities in order to adapt to the human host.
The microbiome restrains melanoma bone growth by promoting intestinal NK and Th1 cell homing to bone (2022)

Bone metastases are frequent complications of malignant melanoma leading to reduced quality of life and significant morbidity. Regulation of immune cells by the gut microbiome influences cancer progression, but the role of the microbiome in tumor growth in bone is unknown. Using intracardiac or intratibial injections of B16-F10 melanoma cells into mice, we showed that gut microbiome depletion by broad-spectrum antibiotics accelerated intraosseous tumor growth and osteolysis. Microbiome depletion blunted melanoma-induced expansion of intestinal NK cells and Th1 cells and their migration from the gut to tumor-bearing bones. Demonstrating the functional relevance of immune cell trafficking from the gut to the bone marrow (BM) in bone metastasis, blockade of S1P-mediated intestinal egress of NK and Th1 cells, or inhibition of their CXCR3/CXCL9-mediated influx into the BM, prevented the expansion of BM NK and Th1 cells and accelerated tumor growth and osteolysis. Using a mouse model, this study revealed mechanisms of microbiota-mediated gut-bone crosstalk that are relevant to the immunological restraint of melanoma metastasis and tumor growth in bone. Microbiome modifications induced by antibiotics might have negative clinical consequences in patients with melanoma.
Activation of platelet‐derived growth factor pathway in human asthmatic pulmonary‐derived mesenchymal cells (2003)

Cell cultures of mesenchymal type were obtained from biopsies taken after bronchoscopy from patients with asthma. It was possible to achieve outgrowth of fibroblast-like cells from these lung biopsies, which stained for alpha-smooth actin indicating that they were of myofibroblast type. Morphologically, two types of myofibroblasts could be observed: one intermediate form with more stretched cell shape and lamellipodia protrusions, and one more differentiated compact form of myofibroblast. The intermediate form was the most dominant type in these patients, indicating an active ongoing remodelling process. Further studies showed that platelet-derived growth factor (PDGF) might be the factor that stimulates the formation of the intermediate type of myofibroblasts, since it enhance migration of normal human lung fibroblasts 4-fold compared to control through an induced formation of stress fibers and lamellipodia protrusions. Additionally, intracellular signalling pathways involved in migration, such as RhoA and MAPkinase were stimulated 1.5-fold and 3.5-fold, respectively. By using two-dimensional (2-D) gel electrophoresis and protein identification by peptide mass finger printing matrix assisted laser desporption/ionization - time of flight - mass spectrometry (MALDI-TOF-MS) it was possible to confirm that PDGF affected the synthesis of proteins involved in the remodelling process, such as collagen VI and post-translational forms thereof. PDGF also stimulated the production of FK506 binding protein of 65 kDa, a protein involved in smooth muscle differentiation, and proteins involved in the rearrangement of the cytoskeleton connected to migration such as the actin related protein ARP3, the T-complex protein and the heat shock protein 60. We demonstrate that PDGF has a potential pathological role in asthma and formation of subepithelial fibrosis by inducing changes in the proteome.
Analysis of transient phase conjugation in photorefractive media (1988)

The response of a photorefractive phase conjugator to time-varying signals is examined. Maxwell’s equations are coupled to the material equations followed by linearization using a strong undepleted pump approximation and simplification by the slowly varying envelope approximation. The resulting set of equations is solved by frequency-domain techniques. The solution is expressed in terms of a transfer function that relates the complex frequencies of the probe and the conjugate field. Limiting forms of the transfer function are derived, and a comparison with a Kerr material is made. The effects of various parameters on the fidelity and stability of the conjugation process are determined. Numerical results are presented showing the distortion of time-varying signals owing to the nonideal conjugation process.
Integrating Recognition and Action Through Task-Relevant Knowledge for Daily Assistive Humanoids (2009)

This paper describes the design of a humanoid robot system that can perform daily assistive tasks. The significant issue in realizing a daily assistive humanoid robot system is to integrate an action subsystem and a recognition subsystem. We have designed a task-relevant knowledge that referred from both the action and recognition subsystems. The knowledge-based subsystem contains not only the information on an environment and objects' shapes, but also represents manipulation and navigation-related knowledge, and that for object recognition and robot localization. Since motion generation and an object recognition procedure share the same knowledge base system, the robot is capable of planning motion while recognizing objects and vice versa. This capability increases the effectiveness and robustness of the system. Three vision-guided behavior controls are presented during the execution of an action: (i) a visual self-localization to recognize the position of the robot, (ii) a visual object localization to update the object location in the model world to generate behaviors and (iii) a visual behavior verification to confirm the success of the motion. Finally, we demonstrated a kitchen service task by multiple humanoid robots to prove the broad capability and applicability of the proposed representation. The knowledge descriptions required for this demonstration consist of 13 behaviors, six objects with manipulation and visual feature knowledge, three search areas for the recognition process and two task-relevant visual behavior verification knowledge bases. We found that the description required for a kitchen service task is rather simple compared to the complexity of the demo scenario.
An unusual outbreak of nontuberculous mycobacteria in hospital respiratory wards: Association with nontuberculous mycobacterial colonization of hospital water supply network (2016)

The incidence and prevalence of pulmonary nontuberculous mycobacterial (NTM) infection is increasing worldwide arousing concerns that NTM infection may become a serious health challenge. We recently observed a significant increase of NTM-positive sputa samples from patients referred to respiratory disease wards of a large tertiary hospital in Rome. A survey to identify possible NTM contamination revealed a massive presence of NTM in the hospital water supply network. After decontamination procedures, NTM presence dropped both in water pipelines and sputa samples. We believe that this observation should encourage water network surveys for NTM contamination and prompt decontamination procedures should be considered to reduce this potential source of infection.
An Estimate of the Steady Magnetic Field Strength Required to Influence Nerve Conduction (1980)

The effects of steady magnetic fields upon the conduction of a nerve impulse are considered. Microscopic chemical effects as well as those due to induced fields are ignored. Arguing from an analysis of transport properties, we show that the minimum magnetic field required to produce observable effects is quite large. For the best case, the rield required to produce a 10 percent reduction in conductivity is roughly 24 T.
Avances en la tecnología de la reducción del cromo (2007)

In 20 of 198 patients with hypertrophic cardiomyopathy, Doppler color flow imaging revealed diastolic paradoxic jet flow across the obliterated left ventricular apex toward the base that suggested the presence of a discrete apical chamber. This prospective study characterized echocardiographic, ventriculographic and scintigraphic findings in these patients, as well as their clinical features. Although echocardiography did not directly show the apical chamber in 13 of the 20 patients, left ventriculography always revealed a small apical outpouching separated from the major basal cavity. Systolic bulging of the apex was always followed by early diastolic shrinkage together with persistent cavity narrowing between the two chambers. After the systolic jet flow, the paradoxic jet flow lasted for 366 +/- 160 ms after aortic valve closure and always extended into the diastolic filling period. The maximal velocity of the paradoxic jet flow occurred during isovolumetric relaxation and the mean velocity was 2 +/- 0.8 m/s, indicating a higher diastolic pressure in the apical chamber than in the main ventricle. Compared with patients who manifested cavity obliteration alone, patients with a paradoxic jet flow more often developed systemic embolism (p less than 0.01), ventricular tachycardia (p less than 0.05) and thallium perfusion abnormalities localized to the apical region (p less than 0.01). Thus, paradoxic jet flow could be an important marker of concealed apical asynergy and the risk of adverse clinical events. The higher diastolic apical pressure suggested by the flow may contribute to the development of an apical aneurysm, even in the absence of fixed coronary artery disease.
Serum interleukin-6, tumor necrosis factor-α and adiponectin levels in Kawasaki disease (2010)

Purpose : Adiponectin is an endogenous modulator of vascular remodeling that suppresses vascular inflammation. However, the role of adiponectin in Kawasaki disease (KD) has not been elucidated. The purpose of this study is to investigate the correlation between serum adiponectin level and several parameters, such as interleukin (IL)-6, tumor necrosis factor (TNF)-?#6944;lipid profile, and C reactive protein (CRP), and to clarify the association between adiponectin and cardiac function. Methods : Twenty-two KD patients (22 patients in acute phase and 20 patients in subacute phase) were enrolled in the study group. The control group consisted of 31 subjects (13 febrile patients and 18 healthy children). Both groups underwent blood sampling and tissue Doppler imaging (TDI). Results : CRP was significantly increased in the KD group compared with the control group. There were no significant differences in serum TNF-?#6944;IL-6, and adiponectin levels between groups. However, a negative correlation was found between adiponectin level and CRP level or platelet count. Systolic myocardial velocity and A myocardial velocity measured by TDI were decreased significantly in the acute KD group compared with the subacute KD group and control group. Positive correlations were found between adiponectin level and systolic myocardial velocity or A myocardial velocity. Conclusion : In acute KD patients, low adiponectin level was related to severe inflammatory reactions and decreased left ventricular functions.
Clinical research and trial registries: the times they are a-changin (2020)

To the Editor In their recent editorial, Tran and Sites advocate greater efforts in identifying discrepancies between registered and reported protocols in manuscripts submitted to RAPM.[1][1] In this invited letter to the editor, we complement their editorial by providing a practical example of the
METHODS: ORIGINAL ARTICLE A Practical Approach to Immunotherapy of Hepatocellular Carcinoma Using T Cells Redirected Against Hepatitis B Virus (2013)

that obtained by retroviral transduction (~18%). Antigen-specific cytokine production of electroporated T cells was efficient within 72-hour period, after which the redirected T cells lost their HBV-specific function. Despite this transient functionality, the TCR-electroporated T cells efficiently prevented tumor seeding and suppressed the growth of established tumors in a xenograft model of HCC. Finally, we established a method for large-scale TCR mRNA electroporation that yielded large numbers of highly functional clinical-grade anti-HBV T cells. This method represents a practical approach to cell therapy of HCC and its inherently self-limiting toxicity suggests potential for application in other HBV-related pathologies.
Aislamiento y caracterización de genes de halotolerancia de remolacha (2003)

Las inmunodeficiencias primarias son un grupo heterogeneo de desordenes caracterizados por defectos en genes implicados en la respuesta inmune. Las inmunodeficiencias congenitas deben diagnosticarse tan pronto sea posible, bien en neonatos o durante el periodo de gestacion. El desarrollo de ensayos clinicos sencillos, rapidos y objetivos que puedan realizarse en cantidades minimas de muestra, conducen al pronto diagnostico. Muchas de las inmunodeficiencias primarias se caracterizan por defectos celulares que son cuantificables por citometria de flujo. El objetivo de este proyecto de tesis ha sido el de relatar nuestra experiencia en el desarrollo de tecnicas que permitan el diagnostico precoz de aquellos individuos afectados por tres inmunodeficiencias congenitas en concreto. Las inmunodeficiencias objeto de estudio tienen en comun que, a pesar de su diversa etiologia, resultan en anormalidades en el numero y/o funciones de los neutrofilos son: * Deficiencia de Adhesion Leucocitaria Tipo I. El procedimiento desarrollado se basa en la demostracion de que las inegrinas Beta2 etan parcial o totalmente ausentes en la superficie de leucocitos estimulados in vitro obtenidos a partir de pacientes afectados por esta inmunodeficiencia. * Enfermedad Granulomatosa Cronica. El ensayo pone de manifiesto la incapacidad de los polimorfonucleares de los pacientes afectados para generar compuestos oxidantes y por tanto destruir a los microorganismos fagocitados. * Sindrome de Hiper-IgM. Mediante este ensayo se demuestra la ausencia del ligando CD40 en linfocitos. T-cooperadores, de forma que los linfocitos B de los individuos afectados, al no ser incluidos a diferenciarse, no experimentan cambios de isotipo y son incapaces de producir IgA. IgC e IgE. Por ultimo, se describe el caso clinico de una paciente diagnosticada con el Sindrome de Hipereosinofilia, que sin ser una inmunodeficiencia, ha sido evaluado mediante diver
Slice Sampling for Probabilistic Programming (2015)

We introduce the first, general purpose, slice sampling inference engine for probabilistic programs. This engine is released as part of StocPy, a new Turing-Complete probabilistic programming language, available as a Python library. We present a transdimensional generalisation of slice sampling which is necessary for the inference engine to work on traces with different numbers of random variables. We show that StocPy compares favourably to other PPLs in terms of flexibility and usability, and that slice sampling can outperform previously introduced inference methods. Our experiments include a logistic regression, HMM, and Bayesian Neural Net.