gebhart/patrepeval huggingface dataset:
Development of a contextually appropriate, reliable and valid basic Wheelchair Service Provision Test (2016)

The results indicate that the Wheelchair Service Provision - Basic Test is a valid method for measuring basic competency of wheelchair professionals. Additionally, researchers recommend a skills assessment to help to ensure only qualified wheelchair professionals receive the certificate. Implications for Rehabilitation Identify key steps in contextually appropriate test development. Describe the components of a contextually appropriate, reliable, and valid test for basic wheelchair service provision. Identify methods used for contextually appropriate test validation.
GaN-on-Silicon integration technology (2012)

This work presents our recent progress on addressing two major challenges to realizing GaN-Silicon integration namely epitaxial growth of GaN-on-Silicon and CMOS-compatible process. We have successfully demonstrated 0.3-μm gate-length GaN HEMTs on 8-inch Si(111) substrate with f <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">T</sub> of 28GHz and f <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">max</sub> of of 64GHz. These device performances are comparable to our reported devices fabricated on 4-inch Si substrate. We have also developed a GaN HEMT process with CMOS-compatible non-gold metal scheme. Excellent ohmic contacts (R <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">c</sub> =0.24 Ω-mm) with smooth surface morphology have been achieved which are comparable to those using conventional III-V gold-based ohmic contacts. 0.15-μm gate-length GaN HEMTs fabricated with this process achieved f <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">T</sub> and f <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">max</sub> of 51 GHz and 50GHz respectively. The 5nm-thick AlGaN barrier HEMT exhibited three terminal OFF-state breakdown voltage (BV <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">gd</sub> ) of 83 V. Our results demonstrate the feasibility of realizing CMOS-compatible high performance GaN HEMTs on 8-inch silicon substrates for future GaN-on-Si integration.
Overexpression of the phage lambda lysozyme cloned in <i>Escherichia coli</i>: use of a degenerative mixture of synthetic ribosome binding sites and increase of the protein stability <i>in vivo</i> (1991)

The R gene of the phage lambda coding for a lysozyme expressed at the end of an infection cycle in Escherichia coli has been cloned in a series of vector plasmids. Two methods for improving the efficiency of translation have been tested. First, the use of a bicistronic construction in which the ribosome binding site (RBS) of the first cistron is that of a highly expressed gene or the use of a degenerate mixture of synthetic oligonucleotides for the optimization of a RBS. The second strategy is more efficient: the analysis of a number of clones reveals that the LaL expression levels are increased by a factor between 3 and 6 times compared with the clone using the natural RBS. The expression levels are described by an approximately Gaussian histogram. The translation promoter that was found to afford the best expression (PL) is under the control of a thermolabile repressor. Under the expression conditions, the protein is partially proteolysed. The proteolysis is significantly decreased by adding salt to the growth medium. After optimization, an increase in expression by a factor of 40 is obtained compared with the initial conditions. An efficient purification protocol is described.
Generation of procoagulant activity (PCA) by phorbol-esters-induced macrophages derived from a leukemic promyelocytic cell line (HL-60) (1982)

Normal human monocytes and macrophages, as well as leukemic promyelocytes, generate potent procoagulant activity (PCA) resembling thromboplastin. In the present study only mild PCA was detected in a leukemic promyelocytic cell line (HL-60) and in promyelocytic cells induced to differentiate into neutrophils by dimethylsulfoxide (DMSO). After exposure to 12-0-tetradecanoylphorbol-13-acetate (TPA), the leukemic promyelocytic cells assume characteristics specific to monocytes and macrophages. This differentiation was associated with potent PCA generation. No PCA was found in lymphoblasts and several lymphoid cell lines after exposure to TPA. The PCA in TPA-induced HL-60 cells resembles tissue thromboplastin and normal monocyte PCA in several aspects: (A) All three accelerate clotting through the extrinsic coagulation pathway; (B) all three are inhibited by concanavalin-A and protected by methyl-α-D-mannopyranoside, suggesting that the PCA contains carbohydrate moieties; (C) the PCA is located in the cell membrane. However, the mechanism of PCA generation in normal monocytes and in TPA-induced HL-60 cells differs in several aspects: (A) Endotoxin and phytohemagglutinin, which are potent stimuli of PCA generation in normal monocytes, have no effect on TPA-induced HL-60 cells; (B) HL-60 cells treated with TPA develop PCA before acquiring any other specific characteristic of monocyte or macrophages; (C) the development of PCA in TPA-induced macrophages is not dependent on cell adhesion or the presence of T cells. In spite of these differences, the production of PCA is additional evidence of the similarity between TPA-induced macrophages and normal human monocytes and macrophages. The HL-60 cells may provide a unique model for studying PCA generation in mononuclear cells.
Anti-tumour activity of fatty acid maltotriose esters (2005)

The anti-tumour properties of two fatty acid esters of maltotriose, 6’’-O-lauroylmaltotriose and 6’’-O-palmitoylmaltotriose, in which the 6-OH at the non-reducing end of the maltotriose has been regioselectively acylated, were studied. Both compounds were synthesised by transesterification of vinyl laurate or vinyl palmitate with maltotriose, in presence of immobilized lipase from Thermomyces lanuginosus. A mixture formed by 2-methyl-2-butanol and dimethylsulfoxide (80:20 v/v) was the reaction medium. Their cytotoxic activities against two human cancer cell lines, Hep-G2 and HeLa, were studied. 6’’-O-palmitoylmaltotriose showed 50% inhibition values (IC50) of 2.3 M (1.7 g/ml) for Hep-G2 and 3.6 M (2.7 g/ml) for HeLa cells, whereas 6’’-O-lauroylmaltotriose displayed a lower inhibitory effect. 6’’-O-palmitoylmaltotriose showed a marginal cytotoxicity to rat hepatocytes, confirming its potential as a new anti-tumour agent.
Multi-channel intraneural vagus nerve recordings with a novel high-density carbon fiber microelectrode array (2020)

Autonomic nerves convey essential neural signals that regulate vital body functions. Recording clearly distinctive physiological neural signals from autonomic nerves will help develop new treatments for restoring regulatory functions. However, this is very challenging due to the small nature of autonomic nerves and the low-amplitude signals from their small axons. We developed a multi-channel, high-density, intraneural carbon fiber microelectrode array (CFMA) with ultra-small electrodes (8-9 µm in diameter, 150-250 µm in length) for recording physiological action potentials from small autonomic nerves. In this study, we inserted CFMA with up to 16 recording carbon fibers in the cervical vagus nerve of 22 isoflurane-anesthetized rats. We recorded action potentials with peak-to-peak amplitudes of 15.1-91.7 µV and signal-to-noise ratios of 2.0-8.3 on multiple carbon fibers per experiment, determined conduction velocities of some vagal signals in the afferent (0.7-4.4 m/s) and efferent (0.7-8.8 m/s) directions, and monitored firing rate changes in breathing and blood glucose modulated conditions. Overall, these experiments demonstrated that CFMA is a novel interface for in-vivo intraneural action potential recordings. This work is considerable progress towards the comprehensive understanding of physiological neural signaling in vital regulatory functions controlled by autonomic nerves.
Optimisation pharmacologique des dérivés de la créatine pour le traitement du déficit en transporteur de la créatine (2013)

Creatine transporter deficiency is a rare brain disease associated with the loss of function of the SLC6A8 (creatine transporter) leading to an absence of creatine at the cerebral level and to a dramatic neurodevelopmental retardation in the children. To date, no effective therapy is available.A potential therapeutic option would be the design of a pharmaceutical formulation of lipophilic prodrugs of creatine that will cross the cell membranes passively and target the neurons in order to restore the creatine content inside these cells.One of the main purposes of this dissertation is to propose an original chemistry synthesis process of creatine esters with long aliphatic chain. These compounds show interesting pharmacological properties of structure-activity relationship between the length of the aliphatic chain (i.e. lipophilicity) and the ability for the drug to enter cerebral endothelial, astroglial and neuronal cells. According to our experimental observations, the dodecyl ester creatine seems to be the best drug candidate. Moreover, the dodecyl ester is acted on by cellular esterases inside patients’ fibroblasts with a functional deficit of the SLC6A8 and increases the intracellular creatine content.The pharmaceutical formulation developed in this study consists by incorporation of dodecyl ester inside a nanovector (Lipid NanoCapsules). Two main advantages can be gained by nanovectorization: firstly, the dodecyl ester is protected from the degradation by plasmatic esterases before reaching the brain. Secondly, the nanovectorization strategy is highly valuable to brain targeting bypassing the blood-brain barrier, which remains until now a major impediment in the drug design for the Central Nervous System. Our experimental observations highlight this two-step therapeutic strategy for the treatment of deficiency of the creatine transporter.This work was financially supported by the International PhD Program of the Life Sciences division of the CEA and the Fondation Jérôme Lejeune.
Selective Behavior in Online Social Networks (2012)

According to the classical communication theories, known as Gate keeping and Selective Exposure, individuals tend to have selective behavior when they disseminate and receive information based on their psychological preferences. Selective behavior related to these two theories have been broadly studied separately. While, thanks to the advent of Online Social Networks (OSNs), larger-scale feedback and user information can be collected. In this paper, based on these data, We analyze the correlation among users' properties (such as age, gender, and cultural background) and analyze their selective behavior by tagging users as disseminators and/or audiences in YouTube, Flickr, and Twitter. We find that despite enormous amount of content available in OSNs, users have a comparatively small selective range and do exhibit selective behavior properties. In particular, they pay the most attention to the content published by disseminators that share similar properties, i.e., gender, age, and country. Nonetheless, we also find significant differences and commonalities among the three OSNs with respect to selective behavior. In particular, (i) the proportion and properties of disseminators, audiences, and dual-role users are quite different for the three networks, (ii) the global level of information spread in Flickr is almost two times than that in Twitter and YouTube is approximately the median one, (iii) For a given country, the global level of information spread is different for different OSNs. For a given OSN, it is different for different countries, (iv) despite ubiquitous presence of dual-role users in OSNs, most of such users are very active as either disseminators or audiences, but not both. Our findings are not only useful for understanding these two theories, but also have applications ranging from advertising and recommendation systems to developing predicting models.
Supplementary Fig. S3 from Effects of Tumor Microenvironment Heterogeneity on Nanoparticle Disposition and Efficacy in Breast Cancer Tumor Models (2023)

&lt;p&gt;Supplementary Fig. S3. Profiling of collagen in basal-like C3-TAg model and claudin-low T11 model at baseline and at 96 h after administration of PLD or NL-doxo at 6 mg/kg I.V. x 1 via tail vein&lt;/p&gt;
Asymmetric Synthesis of α-Alkylamino Acid by Chiral Isocyano Amide Reagent (1985)

Journal Article Asymmetric Synthesis of α-Alkylamino Acid by Chiral Isocyano Amide Reagent Get access Yoshihiro Yamamoto, Yoshihiro Yamamoto Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture, Sakai, Osaka 591, Japan Search for other works by this author on: Oxford Academic Google Scholar Mitsunori Kirihata, Mitsunori Kirihata Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture, Sakai, Osaka 591, Japan Search for other works by this author on: Oxford Academic Google Scholar Itsuo Ichimoto, Itsuo Ichimoto Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture, Sakai, Osaka 591, Japan Search for other works by this author on: Oxford Academic Google Scholar Hiroo Ueda Hiroo Ueda Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture, Sakai, Osaka 591, Japan Search for other works by this author on: Oxford Academic Google Scholar Agricultural and Biological Chemistry, Volume 49, Issue 7, 1 July 1985, Pages 2191–2193, https://doi.org/10.1080/00021369.1985.10867047 Published: 01 July 1985 Article history Received: 22 November 1984 Published: 01 July 1985
Metabolic products of microorganisms. 163. Desferritriacetylfusigen, an antibiotic from Aspergillus deflectus. (1977)

Aspergillus deflectus CBS 109.55 when grown on an iron-free medium produces several antibiotics; one of these was isolated and identified as desferritriacetylfusigen. It inhibits the growth of bacteria, whereas yeasts and fungi are not or only weakly affected.
Configuration and Assessment of a Submersible Fluorometer for Evaluating Fluorescent Dye Deposition (2022)

ABSTRACT Fluorescent tracer dyes are popularly used in agricultural spray application studies to evaluate on-target canopy deposition and off-target drift, and the methods/instruments used for evaluating dye deposit must assure reliability of the data generated. A laboratory setup was configured to adapt a submersible fluorometer for controlled and reliable measurement of pyranine dye fluorescence in liquid samples. The system setup was blanked using a clean sample of de-ionized water and calibrated using standard dye solutions of known concentration (100 to 1,000,000 parts per trillion [ppt]) made from two dyes of different levels of purity (pyranine 98 % and pyranine 85 %). Pyranine 85 % fluorescence readings were consistently lower with reduction factor averaging ∼0.62 times that of pyranine 98 % within a range from 500 to 1,000,000 ppt. The results were verified using a standard laboratory fluorometer. Spray deposition assessment of string, plastic card, cotton ribbon, and artificial foliage samplers was accomplished using the configured fluorometer system setup. The results showed no significant difference among dye purity levels (P = 0.430), no statistically significant interaction between dye purity and concentration (P = 0.484), and no statistically significant interaction between dye purity and sampler type (P = 0.173). Consequently, the configured setup can produce measurements with similar quality as the standard laboratory fluorometer and both dyes tested may be equally used for spray deposition and drift assessments, or for similar applications.
Tim-3+ T-bet+ Tumor-Specific Th1 Cells Colocalize with and Inhibit Development and Growth of Murine Neoplasms (2005)

Although T cells infiltrate many types of murine and human neoplasms, in many instances tumor-specific cytotoxicity is not observed. Strategies to stimulate CTL-mediated antitumor immunity have included in vitro stimulation and/or genetic engineering of T cells, followed by adoptive transfer into tumor-bearing hosts. In this model of B cell lymphoma in SJL/J mice, we used Tim-3(+) T-bet(+) Th1 cells to facilitate the development of tumor-specific CTL. Tumor-specific Th1 cell lines were polarized with IL-12 during in vitro stimulation and long term maintenance. As few as 5 million Tim-3(+) T-bet(+) Th1 cells enabled recipients to resist growth of malignant transplantable cells. In addition, similar numbers of Th1 cells injected into 2- to 3-mo-old mice inhibited development of the spontaneous primary lymphomas, which normally arise in 90% of aging mice. CFSE(+) Th1 cells colocalized with injected tumor cells in vivo and formed conjugates with the tumor cells within follicles, whereas in nontumor-challenged recipients the CFSE(+) Th1 cells localized only within the T cell zones of the spleen. These results provide evidence that adoptive immunotherapy with Tim-3(+) T-bet(+) tumor-specific Th1 cells can be used to induce host cytotoxic responses that inhibit the development and growth of neoplastic cells.
Bistatic vessel detection from the CODAR SeaSonde (2013)

Large numbers of ships travel up and down the East Coast of the United States daily, ranging from recreational vessels to cargo vessels. Especially busy areas are the approaches to New York Harbor. New York currently is the third-busiest port in the United States and it is growing rapidly [1]. The large number of vessels that travel in and around New York, and the expectation of increased numbers, makes Maritime Domain Awareness important for this region.
Butyrate administration is not sufficient to improve immune reconstitution in antiretroviral-treated SIV-infected macaques (2022)

Defective gastrointestinal barrier function and, in turn, microbial translocation have been identified as significant contributors to persistent inflammation in antiretroviral (ARV)-treated people living with HIV. Metabolic supplementation of short-chain fatty acids (SCFAs), generally produced by the commensal microbiome, may improve these outcomes. Butyrate is a SCFA that is essential for the development and maintenance of intestinal immunity and has a known role in supporting epithelial integrity. Herein we assessed whether supplementation with the dietary supplement sodium butyrate would improve immune reconstitution and reduce inflammation in ARV-treated, simian immunodeficiency virus (SIV)-infected rhesus macaques. We demonstrate that butyrate supplementation does not significantly improve immune reconstitution, with no differences observed in systemic CD4+ T-cell frequencies, T-cell functionality or immune activation, microbial translocation, or transcriptional regulation. Our findings demonstrate that oral administration of sodium butyrate is insufficient to reduce persistent inflammation and microbial translocation in ARV-treated, SIV-infected macaques, suggesting that this therapeutic may not reduce co-morbidities and co-mortalities in treated people living with HIV.
Addressing underrepresentation in physiology research through internship opportunities (2023)

A challenge in the undergraduate physiology landscape is that some students who are underrepresented in the biomedical sciences are not aware of or are unable to take advantage of laboratory research opportunities. This is unfortunate because lab research can provide students with unique skill sets that are beneficial for future success, whether the students remain in research or move on to other careers. These skills include opportunities for critical thinking, improvement of oral and written communication skills, development of teamwork and leadership skills, learning about ethics in biomedical decision making and enhancement of digital literacy among others. As a program that sponsors an undergraduate Human Physiology major that has been successful in attracting underrepresented students (&gt;40% students of color and &gt;60% female) for a major Land-grant university, we challenged ourselves to consider how we might help our students make the most of their physiological interests and potentially improve the demographics of representation in our discipline. Starting in the summer of 2020, a group of faculty began a series of meetings to define an undergraduate program that would attract underrepresented students to lab-based internships, minimize the need for outside work by providing stipends and nurture their academic progress through informal student-faculty interactions. Though delayed by the Covid-19 pandemic, this framework has been used to initiate two new outreach programs. In the summer of 2021, we used this framework to initiate an American Heart Association sponsored research internship opportunity for 3rd and 4th year students. This program which we have named UPRIME is the focus of another abstract at this meeting. The INPUT (INtegrative biology and Physiology Undergraduate Training) program was started in fall of 2022. The program is internally supported and targets 1st and 2nd year undergraduates who represent populations that have been underrepresented in biomedical sciences. These students will be provided laboratory research experiences coupled with opportunities for educational support. A stipend of $3000 per student per semester is offered with the prospect of renewal dependent on a post-semester review. A cohort of six students were selected from a campus wide solicitation for applications into the inaugural INPUT class. These students will begin their projects in January 2023. We look forward to contributing to the scientific growth and development of these students. Perceptions of lab-based research and academic progress will be tracked along with career aspirations as the program progresses. Department of Integrative Biology and Physiology, University of Minnesota Medical School This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.