gebhart/patrepeval huggingface dataset:
Cloning and characterization of BCY1, a locus encoding a regulatory subunit of the cyclic AMP-dependent protein kinase in Saccharomyces cerevisiae. (1987)

We have cloned a gene (BCY1) from the yeast Saccharomyces cerevisiae that encodes a regulatory subunit of the cyclic AMP-dependent protein kinase. The encoded protein has a structural organization similar to that of the RI and RII regulatory subunits of the mammalian cyclic AMP-dependent protein kinase. Strains of S. cerevisiae with disrupted BCY1 genes do not display a cyclic AMP-dependent protein kinase in vitro, fail to grow on many carbon sources, and are exquisitely sensitive to heat shock and starvation.
ChemInform Abstract: The Preparation of 4‐Substituted Pyrylium Salts and Their Use in Dienal Synthesis. (1997)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Figure legends from CD4&lt;sup&gt;+&lt;/sup&gt; T Cell and NK Cell Interplay Key to Regression of MHC Class I&lt;sup&gt;low&lt;/sup&gt; Tumors upon TLR7/8 Agonist Therapy (2023)

&lt;p&gt;Legends of supplementary figures&lt;/p&gt;
A Study of Multimodal Addressee Detection in Human-Human-Computer Interaction (2015)

The goal of addressee detection is to answer the question , “Are you talking to me?” When a dialogue system interacts with multiple users, it is crucial to detect when a user is speaking to the system as opposed to another person. We study this problem in a multimodal scenario, using lexical, acoustic, visual, dialogue state, and beamforming information. Using data from a multiparty dialogue system, we quantify the benefits of using multiple modalities over using a single modality. We also assess the relative importance of the various modalities, as well as of key individual features, in estimating the addressee. We find that energy-based acoustic features are by far the most important, that information from speech recognition and system state is useful as well, and that visual and beamforming features provide little additional benefit. While we find that head pose is affected by whom the speaker is addressing, it yields little nonredundant information due to the system acting as a situational attractor. Our findings would be relevant to multiparty, open-world dialogue systems in which the agent plays an active, conversational role, such as an interactive assistant deployed in a public, open space. For these scenarios , our study suggests that acoustic, lexical, and system-state information is an effective and practical combination of modalities to use for addressee detection. We also consider how our analyses might be affected by the ongoing development of more realistic, natural dialogue systems.
Supplemental Table 1 from Bacterial SOS Genes &lt;i&gt;mucAB/umuDC&lt;/i&gt; Promote Mouse Tumors by Activating Oncogenes &lt;i&gt;Nedd9/Aurkb&lt;/i&gt; via a miR-145 Sponge (2023)

&lt;p&gt;Table S1. Synthetic DNA oligomers, PCR primers&lt;/p&gt;
Structure and Raman scattering of chained carbon films on copper substrate: ab initio approach (2018)

During the past decades one-dimensional carbon allotropes were attracting much interest. One of such modifications consists of the sp 1 chains, ordered in a hexagonal array with nearly 5 angstroms interchain spacing -linear-chained carbon (LCC). The technological problem of LCC synthesis is the lack of characterization technique confirming its structure along chains. In this work we try to improve the simple structural model of LCC by adding copper substrate and dopants. We compare the experimental Raman spectra with ones modelled by DFT. The calculation results explain the low-frequency features seen in experimental Raman spectra by the substrate-enhanced interaction of carbon chains.
Simulation of Vehicle-Overhead Power System Interaction on Electric Roads (2012)

Due to the upcoming lack of oil and the environmental problems that conventional internal combustion engines are causing, electric vehicles have gained a growing interest during recent years. One solution to improve the efficiency of the existing road network is to make use of electric roads equipped with an overhead power system, thereby allowing also long-distance truck and bus transports to be powered by electricity without the need of heavy, bulky and expansive batteries. Providing electric power using an overhead power system has primarily been used in railway applications and only to some extent in road applications, for example in the case of trolley buses in urban areas. In this study, an overhead catenary system providing electric power to a long-distance truck by means of a pantograph mechanism that collects power through sliding contact with the overhead wire is analysed through simulation. A model of a truck equipped with a pantograph is developed and its interaction with an overhead catenary system model is simulated using the finite element method. The current collection quality is evaluated by analysing the pantograph-catenary contact force variation during the influence of different disturbances such as road irregularities and contact wire vibrations due to multiple pantographs. The study is an assessment of the possibility of using a conventional overhead power system developed for trains in a new context by providing power to long-distance road transports. The results show that the investigated disturbances influence the dynamics of the studied truck-pantograph-catenary system, nevertheless the contact force variation is within the allowed range according to the technical specifications for interoperability (TSI) for trains. It can be concluded that an overhead power system is a promising solution for a more environmentally friendly energy supply for trucks and buses at specific road sections.
Bile acids regulate MAdCAM-1 expression to link the gut microbiota to cancer immunosurveillance (2023)

In a recent paper in <i>Science</i>, Fidelle et al. unravel a gut immune checkpoint that is subverted by antibiotic treatment. Post-antibiotic dysbiosis of the ileum causes an increase in bile acids that downregulate MAdCAM-1, thereby triggering the exodus of immunosuppressive T cells from gut-associated lymphoid tissues toward tumors.
Effect of some irritants on human epidermal mitosis (1975)

Studies on how irritant materials might induce epidermal hyperplasia were initiated by investigating their influence on epidermal mitosis. 5% hydrochloric acid, neat dimethyl acetamide and 1% benzalkonium chloride had no effect. 5% benzalkonium chloride, however, produced a 10-fold increase in mitotic activity, while a dose response curve was seen with sodium lauryl sulphate (SLS) peaking at 1%. 1% SLS produced a remarkably uniform response for this type of assay and it is suggested that it might provide a useful model for situations of increased epidermal cell turnover such as psoriasis. It is also noted that there was apparently no direct relationship between gross inflammation and the mitotic response.
Spin coating of electrolytes (2023)

Methods for spin coating electrolytic materials onto substrates are disclosed. More particularly, methods for depositing solid coatings of ion-conducting material onto planar substrates and onto electrodes are disclosed. These spin coating methods are employed to fabricate electrochemical sensors for use in measuring, detecting and quantifying gases and liquids.
A random laser with tunable threshold by bending curvature (2019)

We investigate the case that the transport mean free path of emitted photons within the disordered scatterers, which is composed by the ZnO nanowires, is tunable by a curvature bending applied on the flexible substrate underneath, thereby creating a unique light source which is able to be operated above and below the lasing threshold for desirable spectral emissions.
Caudal Ganglionic Eminence Precursor Transplants Disperse and Integrate as Lineage-Specific Interneurons but Do Not Induce Cortical Plasticity (2016)

The maturation of inhibitory GABAergic cortical circuits regulates experience-dependent plasticity. We recently showed that the heterochronic transplantation of parvalbumin (PV) or somatostatin (SST) interneurons from the medial ganglionic eminence (MGE) reactivates ocular dominance plasticity (ODP) in the postnatal mouse visual cortex. Might other types of interneurons similarly induce cortical plasticity? Here, we establish that caudal ganglionic eminence (CGE)-derived interneurons, when transplanted into the visual cortex of neonatal mice, migrate extensively in the host brain and acquire laminar distribution, marker expression, electrophysiological properties, and visual response properties like those of host CGE interneurons. Although transplants from the anatomical CGE do induce ODP, we found that this plasticity reactivation is mediated by a small fraction of MGE-derived cells contained in the transplant. These findings demonstrate that transplanted CGE cells can successfully engraft into the postnatal mouse brain and confirm the unique role of MGE lineage neurons in the induction of ODP.
Supplemental Tables S1-S9 from Spectrum and Degree of CDK Drug Interactions Predicts Clinical Performance (2023)

&lt;p&gt;Table S1. Statistics for the crystallographic analysis; Table S2. Biochemical and cellular potencies of the second generation CDK-directed drug AG-024322; Table S3. In vitro analysis of binding potency of abemaciclib, dinaciclib, and palbociclib to non-kinase proteins; Table S4. Broad kinase selectivity of selective CDK4/6 drugs; Table S5. Biochemical dose-response follow-up was conducted for a subset of kinases inhibited by the three CDK4/6 drugs using a Km concentration of ATP (Carna Biosciences) (CDK proteins excluded); Table S6. Kinase selectivity toward endogenous human kinases using irreversible ATP analog target engagement assay; Table S7. Biochemical potencies of selective CDK4/6 drugs palbociclib (PD-0332991); Table S8. Human pharmacokinetic properties of CDK-targeted drugs; Table S9. Isothermal titration calorimetry results for CDK-directed drugs binding to CDK6 in the absence of cyclin D.&lt;/p&gt;
Nanoformulations with Leishmania braziliensis Antigens Triggered Controlled Parasite Burden in Vaccinated Golden Hamster (Mesocricetus auratus) against Visceral Leishmaniasis (2022)

Leishmaniasis is a widespread vector-borne disease in Brazil, with <i>Leishmania</i> (<i>Leishmania</i>) <i>infantum</i> as the primary etiological agent of visceral leishmaniasis (VL). Dogs are considered the main reservoir of this parasite, whose treatment in Brazil is restricted to the use of veterinary medicines, which do not promote a parasitological cure. Therefore, efficient vaccine development is the best approach to Canine Visceral Leishmaniasis (CVL) control. With this in mind, this study used hamsters (<i>Mesocricetus auratus</i>) as an experimental model in an anti-<i>Leishmania</i> preclinical vaccine trial to evaluate the safety, antigenicity, humoral response, and effects on tissue parasite load. Two novel formulations of nanoparticles made from poly(D, L-lactic) acid (PLA) polymer loading <i>Leishmania braziliensis</i> crude antigen (LB) exhibiting two different particle sizes were utilized: LBPSmG (570 nm) and LBPSmP (388 nm). The results showed that the nanoparticles were safe and harmless to hamsters and were antigenic with the induction in LBSap, LBPSmG, and LBPSmG groups of total anti-<i>Leishmania</i> IgG antibodies 30 days after challenge, which persists 200 days in LBSap and LBPSmP. At the same time, a less pronounced hepatosplenomegaly in LBSap, LBPSmG, and LBPSmP was found when compared to control groups, as well as a less pronounced inflammatory infiltrate and granuloma formation in the spleen. Furthermore, significant reductions of 84%, 81%, and 90% were observed in spleen parasite burden accessed by <i>q</i>PCR in the LBSap, LBPSmG, and LBPSmP groups, respectively. In this way, LBSap, LBPSmG, and LBPSmP formulations showed better results in vaccinated and <i>L. infantum</i>-challenged animals in further reducing parasitic load in the spleen and attenuating lesions in liver and splenic tissues. This results in safe, harmless nanoformulation vaccines with significant immunogenic and infection control potential. In addition, animals vaccinated with LBPSmP had an overall reduction in parasite burden in the spleen, indicating that a smaller nanoparticle could be more efficient in targeting antigen-presenting cells.
Spatial Distribution of Full-Field Residual Stress and Its Correlation with Fracture Strength of Thin Silicon Wafers (2013)

Residual stresses are a major concern in thin silicon wafers because of their potentially detrimental effect on mechanical properties during cell processing. Residual stresses are induced by crystal growth as well as wafering. This work investigates the correlation between the residual stress distribution in a mono-cast silicon wafer and its fracture strength variation. Full-field residual stress maps in 156 mm square as-cut mono-cast silicon wafers are measured using near infra-red polariscopy and correlated with the spatial variation of fracture strength measured using four-line bending. Results show that regions of the wafer with higher residual shear stress are correlated with lower fracture strength. Etching, which removes saw damage, shows a decrease in residual stress with a corresponding increase in fracture strength. The variation in residual stress and fracture strength in a given wafer also appears to be correlated with the grain structure in that region.
Variability of Microcirculatory Measurements in Critically Ill Patients (2019)

GlycoCheck can be used to study microcirculation. However, to obtain reliable results three consecutive measurements should be performed and averaged. The variation of the measurements currently hampers the clinical application in individual patients.
ChemInform Abstract: The Modern Interpretation of the Wittig Reaction Mechanism (2013)

Abstract Review: 108 refs.
Cerebral magnetic resonance angiography (1992)

Magnetic resonance angiography (MRA) is an accurate non-invasive tool for imaging the cerebral vessels. It provides morphologic information about the cerebral vessels relying on blood flow as the physical basis for generating contrast between stationary tissues and moving spins. 'Selective' MRA gives functional information about the cerebrovascular system such as flow direction, origin of flow, and presence or absence of collaterals. Arteries and veins can be imaged selectively due to their usually opposite flow directions. Although at a relatively early stage of development, MRA has already become a widely used tool for the study of the cerebrovascular system.
Quadratic to linear magnetoresistance tuning in <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:msub><mml:mi>TmB</mml:mi><mml:mn>4</mml:mn></mml:msub></mml:math> (2019)

The change of a material's electrical resistance (R) in response to an external magnetic field (B) provides subtle information for the characterization of its electronic properties and has found applications in sensor and storage related technologies. In good metals, Boltzmann's theory predicts a quadratic growth in magnetoresistance (MR) at low B and saturation at high fields. On the other hand, a number of nonmagnetic materials with weak electronic correlation and low carrier concentration for metallicity, such as inhomogeneous conductors, semimetals, narrow gap semiconductors and topological insulators, and two dimensional electron gas, show positive, nonsaturating linear magnetoresistance (LMR). However, observation of LMR in single crystals of a good metal is rare. Here we present low-temperature, angle-dependent magnetotransport in single crystals of the antiferromagnetic metal, ${\mathrm{TmB}}_{4}$. We observe large, positive, and anisotropic MR(B), which can be tuned from quadratic to linear by changing the direction of the applied field. In view of the fact that isotropic, single crystalline metals with large Fermi surface (FS) are not expected to exhibit LMR, we attribute our observations to the anisotropic FS topology of ${\mathrm{TmB}}_{4}$. Furthermore, the linear MR is found to be temperature independent, suggestive of quantum mechanical origin.
Forecasting Convective Initiation by Monitoring the Evolution of Moving Cumulus in Daytime GOES Imagery (2006)

Abstract This study identifies the precursor signals of convective initiation within sequences of 1-km-resolution visible (VIS) and 4–8-km infrared (IR) imagery from the Geostationary Operational Environmental Satellite (GOES) instrument. Convective initiation (CI) is defined for this study as the first detection of Weather Surveillance Radar-1988 Doppler (WSR-88D) reflectivities ≥35 dBZ produced by convective clouds. Results indicate that CI may be forecasted ∼30–45 min in advance through the monitoring of key IR fields for convective clouds. This is made possible by the coincident use of three components of GOES data: 1) a cumulus cloud “mask” at 1-km resolution using VIS and IR data, 2) satellite-derived atmospheric motion vectors (AMVs) for tracking individual cumulus clouds, and 3) IR brightness temperature (TB) and multispectral band-differencing time trends. In effect, these techniques isolate only the cumulus convection in satellite imagery, track moving cumulus convection, and evaluate various IR cloud properties in time. Convective initiation is predicted by accumulating information within a satellite pixel that is attributed to the first occurrence of a ≥35 dBZ radar echo. Through the incorporation of satellite tracking of moving cumulus clouds, this work represents a significant advance in the use of routinely available GOES data for monitoring aspects of cumulus clouds important for nowcasting CI (0–1-h forecasts). Once cumulus cloud tracking is established, eight predictor fields based on Lagrangian trends in IR data are used to characterize cloud conditions consistent with CI. Cumulus cloud pixels for which ≥7 of the 8 CI indicators are satisfied are labeled as having high CI potential, assuming an extrapolation of past trends into the future. Comparison to future WSR-88D imagery then measures the method's predictive skill. Convective initiation predictability is demonstrated using several convective events—one during IHOP_2002—that occur over a variety of synoptic and mesoscale forcing regimes.
Prevalence of Intrathecal Acyclovir Resistant Virus in Herpes Simplex Encephalitis Patients (2016)

Herpes simplex encephalitis (HSE) is a life-threatening complication of herpes simplex virus (HSV) infection. Acyclovir (ACV) is the antiviral treatment of choice, but may lead to emergence of ACV-resistant (ACVR) HSV due to mutations in the viral UL23 gene encoding for the ACV-targeted thymidine kinase (TK) protein. Here, we determined the prevalence of intrathecal ACVR-associated HSV TK mutations in HSE patients and compared TK genotypes of sequential HSV isolates in paired cerebrospinal fluid (CSF) and blister fluid of mucosal HSV lesions. Clinical samples were obtained from 12 HSE patients, encompassing 4 HSV type 1 (HSV-1) and 8 HSV-2 encephalitis patients. HSV DNA load was determined by real-time PCR and complete HSV TK gene sequences were obtained by nested PCR followed by Sanger sequencing. All HSV-1 HSE patients contained viral TK mutations encompassing 30 unique nucleotide and 13 distinct amino acid mutations. By contrast, a total of 5 unique nucleotide and 4 distinct amino acid changes were detected in 7 of 8 HSV-2 patients. Detected mutations were identified as natural polymorphisms located in non-conserved HSV TK gene regions. ACV therapy did not induce the emergence of ACVR-associated HSV TK mutations in consecutive CSF and mucocutaneous samples of 5 individual patients. Phenotypic susceptibility analysis of these mucocutaneous HSV isolates demonstrated ACV-sensitive virus in 2 HSV-1 HSE patients, whereas in two HSV-2 HSE patients ACVR virus was detected in the absence of known ACVR-associated TK mutations. In conclusion, we did not detect intrathecal ACVR-associated TK mutations in HSV isolates obtained from 12 HSE patients.
Utilisation des noeuds DecaWiNo pour la localisation indoor précise d'objets communicants en UWB (2016)

Une des applications privilégiées de l'Ultra Wide Band (UWB) est la localisation indoor de noeuds communicants. Le mécanisme d'évaluation de la distance entre les noeuds (ranging) permet d'alimenter des algorithmes et des calculs géométriques afin de déterminer la position de noeuds communicants avec une précision d'une dizaine de centimètres. Les travaux de recherche présentés dans cet article s'inscrivent dans cette thématique et visent à utiliser les liens radio de type UWB, proposés par l'amendement [1] de l'année 2007 de la norme IEEE 802.15.4. Nous présentons les noeuds DecaWino, basés sur un tranceiver DecaWave [2] DW1000, et un processeur Cortex-M4 Freescale MK20DX256VLH7, présent sur le module Teensy 3.2 de l'écosystème Arduino. Associés aux noeuds DecaWino que nous avons conçus, notre plateforme logicielle Decaduino [3], offre un environnement logiciel [4] adapté et permet le prototypage rapide de solutions de localisation de noeuds UWB.
High throughput microcantilever detector (2023)

In an improved uncoated microcantilever detector, the sample sites are placed on a separate semi-conducting substrate and the microcantilever element detects and measures the changes before and after a chemical interaction or hybridization of the sites by sensing differences of phase angle between an alternating voltage applied to the microcantilever element and vibration of the microcantilever element. In another embodiment of the invention, multiple sample sites are on a sample array wherein an array of microcantilever elements detect and measure the change before and after chemical interactions or hybridizations of the sample sites.
Plant-Derived Exosome-like Nanoparticles for Biomedical Applications and Regenerative Therapy (2023)

Plant-derived exosome-like nanoparticles (PDENs) comprise various bioactive biomolecules. As an alternative cell-free therapeutic approach, they have the potential to deliver nano-bioactive compounds to the human body, and thus lead to various anti-inflammatory, antioxidant, and anti-tumor benefits. Moreover, it is known that Indonesia is one of the herbal centers of the world, with an abundance of unexplored sources of PDENs. This encouraged further research in biomedical science to develop natural richness in plants as a source for human welfare. This study aims to verify the potential of PDENs for biomedical purposes, especially for regenerative therapy applications, by collecting and analyzing data from the latest relevant research and developments.
Isolation of Functional Presynaptic Complexes from CNS Neurons: A Cell-Free Preparation for the Study of Presynaptic Compartments <i>In Vitro</i> (2010)

The difficulty in developing successful treatments to facilitate nerve regeneration has prompted a number of new in vitro experimental methods. We have recently shown that functional presynaptic boutons can be formed when neuronal cells are cocultured with surface-modified artificial substrates including poly(d-lysine)-coated beads and supported lipid bilayer-coated beads (Lucido(2009) J. Neurosci.29, 12449-12466; Gopalakrishnan(2010) ACS Chem. Neurosci.1, 86-94). We demonstrate here, using confocal microscopy combined with immunocytochemistry, that it is possible to isolate such in vitro presynaptic endings in an exclusive fashion onto glass substrates through a simple "sandwich/lift-off" technique (Perez(2006) Adv. Funct. Mater.16, 306-312). Isolated presynaptic complexes are capable of releasing and recycling neurotransmitter in response to an external chemical trigger. These bead-presynaptic complexes are facile to prepare and are readily dispersible in solution. They are thus compatible with many experimental methods whose focus is the study of the neuronal presynaptic compartment.