gebhart/patrepeval huggingface dataset:
Diastereoselectivity in the homogeneous cyclopolymerization of 1,5-hexadiene (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDiastereoselectivity in the homogeneous cyclopolymerization of 1,5-hexadieneLuigi Resconi and Robert M. WaymouthCite this: J. Am. Chem. Soc. 1990, 112, 12, 4953–4954Publication Date (Print):June 1, 1990Publication History Published online1 May 2002Published inissue 1 June 1990https://pubs.acs.org/doi/10.1021/ja00168a048https://doi.org/10.1021/ja00168a048research-articleACS PublicationsRequest reuse permissionsArticle Views555Altmetric-Citations149LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Functional variants in TBX2 are associated with a syndromic cardiovascular and skeletal developmental disorder (2018)

The 17 genes of the T-box family are transcriptional regulators that are involved in all stages of embryonic development, including craniofacial, brain, heart, skeleton and immune system. Malformation syndromes have been linked to many of the T-box genes. For example, haploinsufficiency of TBX1 is responsible for many structural malformations in DiGeorge syndrome caused by a chromosome 22q11.2 deletion. We report four individuals with an overlapping spectrum of craniofacial dysmorphisms, cardiac anomalies, skeletal malformations, immune deficiency, endocrine abnormalities and developmental impairments, reminiscent of DiGeorge syndrome, who are heterozygotes for TBX2 variants. The p.R20Q variant is shared by three affected family members in an autosomal dominant manner; the fourth unrelated individual has a de novo p.R305H mutation. Bioinformatics analyses indicate that these variants are rare and predict them to be damaging. In vitro transcriptional assays in cultured cells show that both variants result in reduced transcriptional repressor activity of TBX2. We also show that the variants result in reduced protein levels of TBX2. Heterologous over-expression studies in Drosophila demonstrate that both p.R20Q and p.R305H function as partial loss-of-function alleles. Hence, these and other data suggest that TBX2 is a novel candidate gene for a new multisystem malformation disorder.
Supplementary Table 6 from Correlative Analyses of the SARC028 Trial Reveal an Association Between Sarcoma-Associated Immune Infiltrate and Response to Pembrolizumab (2023)

&lt;p&gt;Comparison of tumor PD-L1 expression and tumor immune infiltrate between baseline biopsies and at 8 weeks on treatment (bone only)&lt;/p&gt;
Shotgun proteomics implicates protease inhibition and complement activation in the antiinflammatory properties of HDL (2007)

HDL lowers the risk for atherosclerotic cardiovascular disease by promoting cholesterol efflux from macrophage foam cells. However, other antiatherosclerotic properties of HDL are poorly understood. To test the hypothesis that the lipoprotein carries proteins that might have novel cardioprotective activities, we used shotgun proteomics to investigate the composition of HDL isolated from healthy subjects and subjects with coronary artery disease (CAD). Unexpectedly, our analytical strategy identified multiple complement-regulatory proteins and a diverse array of distinct serpins with serine-type endopeptidase inhibitor activity. Many acute-phase response proteins were also detected, supporting the proposal that HDL is of central importance in inflammation. Mass spectrometry and biochemical analyses demonstrated that HDL3 from subjects with CAD was selectively enriched in apoE, raising the possibility that HDL carries a unique cargo of proteins in humans with clinically significant cardiovascular disease. Collectively, our observations suggest that HDL plays previously unsuspected roles in regulating the complement system and protecting tissue from proteolysis and that the protein cargo of HDL contributes to its antiinflammatory and antiatherogenic properties.
Power Consumption of Fault Tolerant Busses (2008)

<para xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> On-chip interconnects in very deep submicrometer technology are becoming more sensitive and prone to errors caused by power supply noise, crosstalk, delay variations and transient faults. Error-correcting codes (ECCs) can be employed in order to provide signal transmission with the necessary data integrity. In this paper, the impact of ECCs to encode the information on a very deep submicrometer bus on bus power consumption is analyzed. To fulfill this purpose, both the bus wires (with mutual capacitances, drivers, repeaters and receivers) and the encoding–decoding circuitry are accounted for. After a detailed analysis of power dissipation in deep submicrometer fault-tolerant busses using Hamming single ECCs, it is shown that no power saving is possible by choosing among different Hamming codes. A novel scheme, called Dual Rail, is then proposed. It is shown that Dual Rail, combined with a proper bus layout, can provide a reduction of energy consumption. In particular, it is shown how the passive elements of the bus (bottom and mutual wire capacitances), active elements of the bus (buffers) and error-correcting circuits contribute to power consumption, and how different tradeoffs can be achieved. The analysis presented in this paper has been performed considering a realistic bus structure, implemented in a standard 0.13-<formula formulatype="inline"><tex>$\mu{\hbox{m}}$</tex> </formula> CMOS technology. </para>
Three-guide directional coupler as polarisation independent optical switch (1991)

The polarisation independent directional coupler switch with three coupled waveguides is demonstrated. The device exhibits unproved voltage tolerance over existing designs.
Measurement of Avian Cytokines with Real-Time RT-PCR Following Infection with the Avian Influenza Virus (2008)

Functional and molecular techniques have both been employed to measure the production of cytokines following influenza infection. Historically, the use of functional or antibody-based techniques was employed in mammalian immunology. In avian immunology, only a few commercial antibodies are available to measure avian cytokines. However, the determination of the genomic sequence of Gallus gallus species has made it possible to measure cytokine induction without monoclonal antibody- or functional-based tests, but rather based on molecular techniques. Although these tests do not measure functionally expressed cytokines, the lack of reagents to identify and quantify avian cytokines makes them critical to extend any measure of cytokine response. Measurement of cytokine induction, based on the design of primers and probes for RT-PCR or real-time RT-PCR for the cytokine mRNA, has become one of the more recent technologies reported to measure avian cytokines. It is important to note that small nucleotide polymorphisms between different lines of birds may result in substandard results when using published primer and probe sequences. This requires empirical testing to ensure adequate results.
The Saccharomyces cerevisiae DPM1 gene encoding dolichol-phosphate-mannose synthase is able to complement a glycosylation-defective mammalian cell line. (1990)

The Saccharomyces cerevisiae DPM1 gene product, dolichol-phosphate-mannose (Dol-P-Man) synthase, is involved in the coupled processes of synthesis and membrane translocation of Dol-P-Man. Dol-P-Man is the lipid-linked sugar donor of the last four mannose residues that are added to the core oligosaccharide transferred to protein during N-linked glycosylation in the endoplasmic reticulum. We present evidence that the S. cerevisiae gene DPM1, when stably transfected into a mutant Chinese hamster ovary cell line, B4-2-1, is able to correct the glycosylation defect of the cells. Evidence for complementation includes (i) fluorescence-activated cell sorter analysis of differential lectin binding to cell surface glycoproteins, (ii) restoration of Dol-P-Man synthase enzymatic activity in crude cell lysates, (iii) isolation and high-performance liquid chromatography fractionation of the lipid-linked oligosaccharides synthesized in the transfected and control cell lines, and (iv) the restoration of endoglycosidase H sensitivity to the oligosaccharides transferred to a specific glycoprotein synthesized in the DPM1 CHO transfectants. Indirect immunofluorescence with a primary antibody directed against the DPM1 protein shows a reticular staining pattern of protein localization in transfected hamster and monkey cell lines.
Evolution of Iron Based Intermetallic Phases in Al‐7wt%Si Hypoeutectic Alloy (2012)

This chapter contains sections titled: Introduction Background Experiments Results and Discussions Conclusion Acknowledgements
A Novel 1,3 O .fwdarw. C Silyl Shift and Deacylation Reaction Mediated by Tetra-n-butylammonium Fluoride in an Aromatic System (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA Novel 1,3 O .fwdarw. C Silyl Shift and Deacylation Reaction Mediated by Tetra-n-butylammonium Fluoride in an Aromatic SystemHu-Ming He, Phillip E. Fanwick, Karl Wood, and Mark CushmanCite this: J. Org. Chem. 1995, 60, 18, 5905–5909Publication Date (Print):September 1, 1995Publication History Published online1 May 2002Published inissue 1 September 1995https://pubs.acs.org/doi/10.1021/jo00123a029https://doi.org/10.1021/jo00123a029research-articleACS PublicationsRequest reuse permissionsArticle Views263Altmetric-Citations19LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
HomeMaestro: Distributed monitoring and diagnosis of performance anomalies in home networks (2008)

Home networks are comprised of applications running over multiple wired and wireless devices competing for shared network resources. Despite all the devices operating in a single administrative domain in such networks, applications operate independently, and users cannot express or enforce policies. By studying multiple households’ network performance at the packet-level correlated with diaries capturing user experiences, we show that the lack of cooperation across applications leads to observable performance problems and associated user frustration. We describe HomeMaestro, a cooperative host-based system that monitors local and global application performance, and automatically detects contention for network resources. HomeMaestro is designed to manage home and small networks and requires no modification to routers, access points, applications, or protocols. At each host, it transparently monitors per-flow and per-process network usage statistics, such as throughput, RTT, and loss rates. We propose novel algorithms for detecting performance anomalies and application contention using time-series and cross-correlation analysis. Through the collected household traces, we show that HomeMaestro effectively identifies roughly 95% of the user reported problems. In addition, we evaluate the processing and communication overhead of HomeMaestro, which typically are below 5% CPU utilization and 10kbps signalling traffic per host respectively.
&lt;title&gt;Detection of atherosclerosis via magnetic resonance imaging&lt;/title&gt; (1992)

Magnetic resonance imaging (MRI) of atherosclerotic lipids using a stimulated-echo diffusion- weighted (STED) sequence is demonstrated. The STED sequence exploits the large difference in diffusion between lipid (primarily cholesteryl ester) and water. The optimization of the STED sequence is discussed. The results of lipid imaging are corroborated with nuclear magnetic resonance (NMR) spectroscopy. This technique is non-invasive, and therefore, it is potentially useful in following the progression of the disease in animal models and in humans.
Optically Stimulated Luminescence in Tm-Doped Calcium Fluoride Phosphor Crystal for Application to a Novel Passive Type Dosimeter (2015)

An intense optically stimulated luminescence (OSL) with four emission bands in the wavelength range from 290 to 420 nm is observed for the first time in X-ray-irradiated Tm-doped CaF 2 (CaF 2 :Tm) phosphor crystal. The OSL intensity increases almost linearly with increasing X-ray absorbed dose. Thermally stimulated luminescence (TSL) glow curves with four peaks are also observed in X-ray-irradiated CaF 2 :Tm phosphor crystal. It is confirmed that the shape of the TSL glow curve in X-ray-irradiated CaF 2 :Tm phosphor crystal is the same as that of a commercially available dosimeter (TLD-300). An OSL emission and stimulation mechanism of X-ray-irradiated CaF 2 :Tm phosphor crystal is proposed.
Three‐Dimensional Bioprinting Materials with Potential Application in Preprosthetic Surgery (2016)

Current methods in handling maxillofacial defects are not robust and are highly dependent on the surgeon's skills and the inherent potential in the patients' bodies for regenerating lost tissues. Employing custom-designed 3D printed scaffolds that securely and effectively reconstruct the defects by using tissue engineering and regenerative medicine techniques can revolutionize preprosthetic surgeries. Various polymers, ceramics, natural and synthetic bioplastics, proteins, biomolecules, living cells, and growth factors as well as their hybrid structures can be used in 3D printing of scaffolds, which are still under development by scientists. These scaffolds not only are beneficial due to their patient-specific design, but also may be able to prevent micromobility, make tension free soft tissue closure, and improve vascularity. In this manuscript, a review of materials employed in 3D bioprinting including bioceramics, biopolymers, composites, and metals is conducted. A discussion of the relevance of 3D bioprinting using these materials for craniofacial interventions is included as well as their potential to create analogs to craniofacial tissues, their benefits, limitations, and their application.
Construction of a bifunctional Escherichia coli heat-stable enterotoxin (STb)-alkaline phosphatase fusion protein (1990)

A fusion between the genes encoding the Escherichia coli STb heat-stable enterotoxin (estB) and alkaline phosphatase (phoA) was constructed, and the expressed protein product was characterized. The STb-alkaline phosphatase protein (STb-PhoA) had an apparent molecular mass of 50,000 daltons and was detected with both monoclonal anti-alkaline phosphatase and polyclonal anti-STb antibodies. Expression of the gene fusion resulted in high-level production of alkaline phosphatase activity, indicating that STb-PhoA was processed and exported into the periplasm of the E. coli host strain. Amino acid sequence analysis of the hybrid protein yielded the sequence Ser-Thr-Gln-Ser-Asn-Lys-Lys, indicating that STb-PhoA was processed during export in a fashion identical to that of native STb (Y. M. Kupersztoch, K. Tachias, C. R. Moomaw, L. A. Dreyfus, R. G. Urban, C. Slaughter, and S. Whipp, J. Bacteriol. 172: 2427-2432, 1990). STb-PhoA was purified from an expressed bacterial lysate by preparative isoelectric focusing. In a rat ligated intestinal loop model, purified STb-PhoA induced highly significant (P less than 0.002) fluid secretion. In addition, the specific activity of STb-PhoA was nearly identical to that of purified STb. Thus, the STb-PhoA hybrid protein represents a readily obtainable source of biologically active (STb) enterotoxin that may prove useful in studies to determine the mode of toxin action.
Human behavior and comfort during load carrying to autonomous mobile robot (2023)

Abstract Interactions between humans and autonomous mobile robots (AMRs) are expected to grow in smart cities to improve logistics operations, such as depositing packages on AMRs for pickup on the street. However, the way that humans walk and pass objects to an AMR when approaching each other remains largely unknown. We conducted two psychophysical experiments to clarify the behavior and comfort of humans when carrying a package and placing it on an AMR for load carrying. Participants were asked to approach a programmed AMR and pass the package in two experiments: 1) changing the stop distance and AMR speed and 2) changing the stop distance and package weight. Motion trackers quantified the participants’ walking speed and frequency of hesitation to walk. In addition, the subjective heaviness and comfort were recorded through a questionnaire during each trial. The results indicated that the participants’ speed decreased and hesitation probability increased when the stop distance of the AMR decreased. Nevertheless, the participants felt more comfortable with the close approach, whereas the package weight did not affect their behavior. By contrast, they felt uncomfortable when AMR remained still. These findings suggest that humans regard the AMR approach as load-carrying assistance and not as invading their personal space. To achieve a comfortable interaction in load carrying from humans to AMRs, we suggest that the AMR can closely approach a person without eliciting personal space invasion.
Hydrophosphination of Bicyclo[1.1.0]butane-1-carbonitriles (2016)

Hydrophosphination of bicyclo[1.1.0]butyl nitriles with phosphine boranes and phosphites provided novel cyclobutyl-P derivatives. The reaction generally favors the syn-diastereomer, and the nitrile can be reduced and converted to other functional groups, thus enabling the preparation of bidentate ligands that access new conformational space by virtue of their attachment to the torsionally malleable but sterically restrictive cyclobutane scaffold. The enantioselective hydrogenation of dehydrophenylalanine using a bidentate phosphine-phosphite ligand illustrates the synthetic utility of the newly prepared scaffold.
EPSTEIN-BARR VIRUS-ASSOCIATED LYMPHOPROLIFERATIVE DISEASE AND VACCINES (1998)

S27 Epstein-Barr virus (EBV) infection results not only in the acute viral syndrome, infections mononucleosis (IM) but also in a number of geographically defined tumours. These tumours can be characterised in terms of the extent of expression of EBV latent antigens which include the EBV nuclear antigens (EBNA1-6) and the transmembrane proteins, LMP1 and 2. It has become increasingly evident that cytotoxic T lymphocytes (CTL) play a major role in limiting the expansion of EBV-infected B lymphocytes during primary EBV infection and following convalescence. There is very strong focussing of this CTL response towards recognition of epitopes within EBNA2-6 (Rickinson and Moss, 1997) and away from the malignancy-associated transmembrane EBV protein, LMP1. It seems highly likely that the EBV-associated immunohiastic lymphomas (IL) that arise following organ transplantation emerge as a result of the unrestricted proliferation of EBV-infected B cells in the inumunosuppressed graft recipient. Effective treatment of IL arising in bone marrow transplant patients has recently been developed and involves the adoptive transfer of donor CTL with specificity towards epitopes within the EBNA2-6 proteins. At this stage it is not clear the extent to which this procedure will find application in the treatment of IL following solid organ engraftment although early indications are encouraging. The opportunities for effective EBV CTL-based immunotherapy in the treatment of nasopharyngeal carcinoma (NPC) is reduced since the available target antigens are limited to EBNA1 and LMP1. CTL-based therapy will undoubtedly be influenced by the ability of these malignant cells to endogenously process and present target epitopes for immune recognition by CTLs. Analysis of NPC cells in fresh tumour biopsies and short-term established lines revealed normal expression of the MHC-encoded peptide transporters TAP-1 and TAP-2 together with high levels of HLA class 1 alleles on the cell surface suggesting that peptides are available for binding to nascent MHC molecules in the endoplasmic reticulum. The phenotype of the final EBV-associated malignancy to be considered. Burkitt's lymphoma (BL) appears designed to resist CTL recognition. Thus surface expression of MHC class I alleles and TAP1 and 2 expression are down-regulated and EBV antigen expression is restricted to EBNA1. Surprisingly, class II expression on BL cells is high, and efficient processing of CD4-restricted CTL epitopes through this pathway has been demonstrated. It seems likely that effective treatment of BL will revolve around protocols designed to reverse its undifferentiated phenotype. Interestingly, CD40 ligand treatment of BL cells reverses some of the features of the BL phenotype which is associated with CTL resistance.
microRNA-122 amplifies hepatitis C virus translation by shaping the structure of the internal ribosomal entry site (2018)

The liver-specific microRNA-122 (miR-122) recognizes two conserved sites at the 5' end of the hepatitis C virus (HCV) genome and contributes to stability, translation, and replication of the viral RNA. We show that stimulation of the HCV internal ribosome entry site (IRES) by miR-122 is essential for efficient viral replication. The mechanism relies on a dual function of the 5' terminal sequence in the complementary positive (translation) and negative strand (replication), requiring different secondary structures. Predictions and experimental evidence argue for several alternative folds involving the miR-binding region (MBR) adjacent to the IRES and interfering with its function. Mutations in the MBR, designed to suppress these dysfunctional structures indeed stimulate translation independently of miR-122. Conversely, MBR mutants favoring alternative folds show impaired IRES activity. Our results therefore suggest that miR-122 binding assists the folding of a functional IRES in an RNA chaperone-like manner by suppressing energetically favorable alternative secondary structures.