gebhart/patrepeval huggingface dataset:
Absolute Quantification of Residual Solvent in Mesoporous Silica Drug Formulations Using Magic-Angle Spinning NMR Spectroscopy (2022)

Porous silica is used as a drug delivery agent to improve the bioavailability of sparsely soluble compounds. In this approach, the active pharmaceutical ingredient (API) is commonly loaded into the porous silica by incipient wetness impregnation using organic solvents. Subsequent solvent elimination is critical as the residual solvent concentration cannot exceed threshold values set by health and safety regulations (e.g., EMA/CHMP/ICH/82260/2006). For dichloromethane and methanol, for example, residual concentrations must be below 600 and 3000 ppm, respectively. Today, EU and USA Pharmacopoeias recommend tedious procedures for residual solvent quantification, requiring extraction of the solvent and subsequent quantification using capillary gas chromatography with static headspace sampling (sHS-GC). This work presents a new method based on the combination of standard addition and absolute quantification using magic-angle spinning nuclear magnetic resonance spectroscopy (MAS qNMR). The methodology was originally developed for absolute quantification of water in zeolites and has now been validated for quantification of residual solvent in drug formations using mesoporous silica loaded with ibuprofen dissolved in DCM and MeOH as test samples. Interestingly, formulations prepared using as-received or predried mesoporous silica contained 5465 versus 484.9 ppm DCM, respectively. This implies that the initial water content of the silica carrier can impact the residual solvent concentration in drug-loaded materials. This observation could provide new options to minimize the occurrence of these undesired solvents in the final formulation.
Contraction Stress Related to Degree of Conversion and Reaction Kinetics (2002)

Polymerization shrinkage of composites bonded to cavity preparations generates stress on the tooth/restoration interface. The purpose of this study was to verify the influence of degree of conversion and speed of polymerization reaction on contraction stress. We prepared experimental composites with different curing rates by varying the concentration of inhibitor (butylated hydroxytoluene). We verified the effect of degree of conversion by submitting one of the composites to different photo-activation times. Contraction stress was monitored for 10 minutes in a tensilometer. Fourier-transformed infrared spectrometry was used for assessment of the degree of conversion. Volumetric shrinkage was determined by means of a mercury dilatometer. Degree of conversion and volumetric shrinkage showed a non-linear relationship with energy density. Degree of conversion showed a pronounced influence on stress. Increased inhibitor concentration reduced curing rate and contraction stress in composites, without compromising the final degree of conversion.
Recent Developments in Cell-Based Microscale Technologies and Their Potential Application in Personalised Medicine (2011)

It is becoming increasingly apparent that some individuals are more susceptible to disease than others and more importantly some patients respond to prescribed therapies better than others. One of the main reasons for differences in disease susceptibility and the effectiveness of drug treatment lies in the genetic makeup of the patient. In addition to many environmental factors, genetic variations such as mutations, DNA polymorphisms and epigenetic gene regulation are the key players involved in the fate of a person's health. Recent advances in genomics and proteomics are providing novel insights into the complex biological process of disease. These insights will ultimately help to tailor personalised approaches to the treatment of disease based upon individual molecular "blueprints" of their genome and proteome. Personalised medicine extends beyond the traditional medical approach in the treatment of patients as it aims to identify and target molecular factors contributing to the illness of individual patients. The personalised medicine approach is already playing a significant role in the way we treat and monitor disease. As many as 10 out of 36 anti-cancer drugs approved by the European Union in the last 10 years are considered to be personalised medicines Breast cancer is one of the best examples whereby a personalised medical approach is adopted to detect the expression status of an oestrogen receptor called ESR1 in the nucleus of breast cancer cells. Approximately 70% of breast cancer patients overexpress this protein which is an important prognostic and predictive marker. Outcomes for these patients have been significantly improved by targeting the ESR1 using a hormonal treatment known as Tamoxifen. Interestingly this is the most commonly prescribed anticancer treatment in the world, highlighting the importance of a personalised approach in the management of disease. Microscale technologies are emerging as an enabling platform for the development of novel personalised medicines and their broad accessibility. Miniaturised devices have the potential to process minute clinical samples and perform extensive genetic, molecular and cellular analyses directly on a microfluidic chip. The integration of pre-analytical sample handling with a subsequent sample analysis on a single microfluidic device will help to achieve highest reproducibility of results and minimise inter-laboratory bias and operators www.intechopen.com
ChemInform Abstract: Palladium‐ and Platinum‐Catalyzed Addition of Aldehydes with Allylstannanes. (1995)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Hybrid microdisk laser on a silicon platform using lateral-field optoelectronic tweezers assembly (2008)

An InGaAs/InGaAsP microdisk laser is assembled on silicon using lateral-field optoelectronic tweezers, achieving room-temperature pulsed operation with a threshold power of 0.85 mW. The room-temperature assembly enables a post-CMOS process to fabricate micro-lasers on silicon.
Vocalization based Individual Classification of Humpback Whales using Support Vector Machine (2007)

Visual surveys and DNA analysis hold primary importance in marine mammal observation. Especially, recognition of whales and their population density estimation rely heavily on these costly and tedious methods. Acoustic survey of vocalizing cetaceans, in contrast, is emerging as a promising technique for efficient, automatic, non-invasive and convenient observation and analysis of such vocally active organisms. However, it is, yet, too premature to replace the conventional methods altogether. In this paper, we have presented our results on recognition of individual humpback whales based on their vocalization data. Cepstral coefficients from song units extracted from audio records of seven humpback whales, re-sampled at 8KHz, were subjected to cepstral analysis. The extracted coefficients were used to develop multi-class support vector machine (SVM) classifier model. The test phase results indicated classification accuracy as high as 99% as compared to earlier best results of 88%, achieved by Gaussian mixture model (GMM) trained on cepstral coefficients. Furthermore, this improvement was attained with a highly reduced training dataset size. It was also verified that the song duration of approximately 128 seconds was sufficient for reliable identification in test phase for given dataset.
MAGIC upper limits on the GRB 090102 afterglow (2013)

Indications of a GeV component in the emission from GRBs are known since the EGRET observations during the 1990's and they have been confirmed by the data of the Fermi satellite. These results have, however, shown that our understanding of GRB physics is still unsatisfactory. The new generation of Cherenkov observatories and in particular the MAGIC telescope, allow for the first time the possibility to extend the measurement of GRBs from several tens up to hundreds of GeV energy range. Both leptonic and hadronic processes have been suggested to explain the possible GeV/TeV counterpart of GRBs. Observations with ground-based telescopes of very high energy photons (E&amp;gt;30 GeV) from these sources are going to play a key role in discriminating among the different proposed emission mechanisms, which are barely distinguishable at lower energies. MAGIC telescope observations of the GRB 090102 (z=1.547) field and Fermi Large Area Telescope (LAT) data in the same time interval are analysed to derive upper limits of the GeV/TeV emission. We compare these results to the expected emissions evaluated for different processes in the framework of a relativistic blast wave model for the afterglow. Simultaneous upper limits with Fermi and a Cherenkov telescope have been derived for this GRB observation. The results we obtained are compatible with the expected emission although the difficulties in predicting the HE and VHE emission for the afterglow of this event makes it difficult to draw firmer conclusions. Nonetheless, MAGIC sensitivity in the energy range of overlap with space-based instruments (above about 40 GeV) is about one order of magnitude better with respect to Fermi. This makes evident the constraining power of ground-based observations and shows that the MAGIC telescope has reached the required performance to make possible GRB multiwavelength studies in the very high energy range.
How does Université Paris-Saclay support pedagogical transformation and innovation to prepare for the future of education? (2020)

The Université Paris-Saclay integrates in its strategic plan the importance of supporting pedagogical transformation and innovations aimed at the continuous improvement of education and training, student success and actors in professional development. Its Education Department is committed to a proactive policy of providing incentives through a range of complementary actions. For several years now, it has encouraged a collective dynamic in order, on the one hand, to offer times for inter-institutional sharing of these teaching experiences, and, on the other hand, to provide financial support for numerous transformative initiatives. It is also very dedicated to the dissemination and promotion of individual and collective successes related to pedagogy. Several structuring actions are carried out to support pedagogical transformation: setting up a working group, creating a specific mission, allocating a budget, organising an annual pedagogical day and thematic workshops, supporting innovative projects, training for new teachers as soon as they are recruited, accompanying the deployment of the competency- based approach, creating a chair of action-research in pedagogy, communicating and publishing. This paper (adapted from the original poster) focuses on the support of innovative projects.
Airway diffusing capacity of nitric oxide and steroid therapy in asthma (2004)

Exhaled nitric oxide (NO) concentration is a noninvasive index for monitoring lung inflammation in diseases such as asthma. The plateau concentration at constant flow is highly dependent on the exhalation flow rate and the use of corticosteroids and cannot distinguish airway and alveolar sources. In subjects with steroid-naive asthma (n = 8) or steroid-treated asthma (n = 12) and in healthy controls (n = 24), we measured flow-independent NO exchange parameters that partition exhaled NO into airway and alveolar regions and correlated these with symptoms and lung function. The mean (+/-SD) maximum airway flux (pl/s) and airway tissue concentration [parts/billion (ppb)] of NO were lower in steroid-treated asthmatic subjects compared with steroid-naive asthmatic subjects (1,195 +/- 836 pl/s and 143 +/- 66 ppb compared with 2,693 +/- 1,687 pl/s and 438 +/- 312 ppb, respectively). In contrast, the airway diffusing capacity for NO (pl.s-1.ppb-1) was elevated in both asthmatic groups compared with healthy controls, independent of steroid therapy (11.8 +/- 11.7, 8.71 +/- 5.74, and 3.13 +/- 1.57 pl.s-1.ppb-1 for steroid treated, steroid naive, and healthy controls, respectively). In addition, the airway diffusing capacity was inversely correlated with both forced expired volume in 1 s and forced vital capacity (%predicted), whereas the airway tissue concentration was positively correlated with forced vital capacity. Consistent with previously reported results from Silkoff et al. (Silkoff PE, Sylvester JT, Zamel N, and Permutt S, Am J Respir Crit Med 161: 1218-1228, 2000) that used an alternate technique, we conclude that the airway diffusing capacity for NO is elevated in asthma independent of steroid therapy and may reflect clinically relevant changes in airways.
Construction and characterization of transposon Tn<i>phoZ</i>for the identification of genes encoding exported proteins in<i>Streptococcus agalactiae</i> (2004)

Bacterial virulence often depends on exported proteins. To identify genes encoding exported proteins in the neonatal pathogen, group B streptococcus, the transposon TnphoZ was constructed. Here, the coding sequence for the secretion-dependent enzyme alkaline phosphatase from Enterococcus faecalis was fused to the left terminal repeat of Tn917, generating TnphoZ. A collection of TnphoZ mutants was isolated and the DNA flanking the transposon insertion sites was sequenced. Sequence data correlated the expression of high AP activity with transposon insertion into genes encoding predicted exported proteins. It is anticipated that TnphoZ will be suitable for use in other Gram-positive hosts.
Lack of Effects of Tetracaine in Rat Nucleus Paragigantocellularis (PGC) on Analgesia from Periqueductal Gray (PAG) Met-Enkephalin (ME) Injections (In Raphe Magnus-Blocking Doses), and on Baseline Tail-Flick Latency (1994)

We showed previously that (1 microgram) tetracaine block of PAG prevents the analgesic effect of PGC-ME injections, and also produces hyperalgesia regarding baseline tail-flick latencies. In the present study we injected PGC (unilaterally and bilaterally) with tetracaine so as to study its possible role in tonic descending inhibition of pain, as well as its necessity for analgesia due to PAG opiate injection. Two micrograms of tetracaine in PGC unilaterally or bilaterally failed to affect baseline tail-flick latencies and failed to attenuate at all the distinct analgesic effect of 2 micrograms ME injected into PAG. This analgesic effect was seen as a change in mean tail-flick latency from about 5.5 to 7.0 seconds 8-12 minutes post-injection, recovering to 5.5 second baseline by 25 minutes. Two micrograms tetracaine in nucleus raphe magnus (RM) was however sufficient to block the analgesic effect of RM stimulation. Thus PGC does not appear to participate in tonic descending pain inhibition (as PAG appears to do) nor is its functional integrity necessary for PAG ME analgesia (though PAG is necessary for PGC analgesia).
3 Cat-4 mission, 1-Unit CubeSat for earth observation: Evaluation on the qualification and production during Phase D (2022)

The 3Cat-4 mission is a 1-unit CubeSat platform that serves as a technology demonstrator and educational platform for students at Universitat Politècnica de Catalunya (UPC). Promoted by the UPC Nanosatellite and Payload Laboratory (UPC NanoSatLab), the most notable subsystems that innovate in the nanosatellite scenario are (1) the Flexible Microwave Payload - 1 (FMPL-1) [1], a cost-effective payload to execute Global Navigation Satellite System Reflectometry (GNSS-R), and L-band microwave radiometry experiments using a commercial off-the-shelf (COTS) software-defined radio (SDR) and (2) the Nadir Antenna Deployment Subsystem (NADS) [2], an in-orbit deployable high-directivity antenna used by Earth Observation (EO) payloads. This paper presents the findings of the 3Cat-4 mission during Phase D, the qualification and production phase of the project. Since the publication of the first introductory work for this mission in 2019[3], several sections of the subsystems have been redesigned and upgraded to correct previous design flaws or to meet new requirements. In addition, this paper addresses the educational perspective of this mission, analyzing its performance and usefulness in the aforementioned subject.
Computability of the Channel Reliability Function and Related Bounds (2021)

The channel reliability function is an important tool that characterizes the reliable transmission of messages over communication channels. For many channels, only upper and lower bounds of the function are known. We analyze the computability of the reliability function and its related functions. We show that the reliability function is not a Turing computable performance function. The same also applies to the functions related to the sphere packing bound and the expurgation bound. Furthermore, we consider the $R_\infty$ function and the zero-error feedback capacity, since they play an important role in the context of the reliability function. Both the $R_\infty$ function and the zero-error feedback capacity are not Banach Mazur computable.
On the Relative Importance of Visual and Spatial Audio Rendering on VR Immersion (2022)

A study was performed using a virtual environment to investigate the relative importance of spatial audio fidelity and video resolution on perceived audio-visual quality and immersion. Subjects wore a head-mounted display and headphones and were presented with a virtual environment featuring music and speech stimuli using three levels each of spatial audio quality and video resolution. Spatial audio was rendered monaurally, binaurally with head-tracking, and binaurally with head-tracking and room acoustic rendering. Video was rendered at resolutions of 0.5 megapixels per eye, 1.5 megapixels per eye, and 2.5 megapixels per eye. Results showed that both video resolution and spatial audio rendering had a statistically significant effect on both immersion and audio-visual quality. Most strikingly, the results showed that under the conditions that were tested in the experiment, the addition of room acoustic rendering to head-tracked binaural audio had the same improvement on immersion as increasing the video resolution five-fold, from 0.5 megapixels per eye to 2.5 megapixels per eye.
Probing Biomaterials with the Atomic Force Microscope (1999)

Abstract Recent AFM research in our laboratory has covered such diverse biomaterials as laminin and other macromolecules from basement membranes (Fig. 1) (1), DNA condensed for gene therapy (Fig. 2) (2), DNA-protein complexes in the yeast kinetochore (Fig. 3) (3), and biofilms of the bacterium Pseudomonas putida (Fig. 4) (4). Laminin is a major protein of basement membranes. When analyzed by AFM in air, it shows a variety of conformations of its cruciform structure (Fig. 1A). Time-lapse images of a single laminin molecule in aqueous solution show the flexibility of the laminin arms as they move and bend (Fig. 1B)(1). AFM analysis of DNA condensed for receptor-mediated gene therapy poses a puzzle (Fig. 2)(2). Polylysine condenses DNA poorly (Fig. 2A), while AsOR-polylysine condenses DNA into compact toroids and short rods (Fig. 2B). AsOR (asialoorosomucoid) is a 38-kDa glycoprotein with a net negative charge of 5 carboxylic acid groups per molecule. The puzzle is why this negative glycoprotein, covalently attached to polylysine, can enhance the condensation of DNA so much over the condensation caused by the polycation, polylysine, alone.
Circular dichroism of an optically active olefin chromophore: (R)-3-methylcyclopentene (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCircular dichroism of an optically active olefin chromophore: (R)-3-methylcyclopenteneM. Levi, D. Cohen, V. Schurig, Harold Basch, and A. GedankenCite this: J. Am. Chem. Soc. 1980, 102, 23, 6972–6975Publication Date (Print):November 5, 1980Publication History Published online1 May 2002Published inissue 5 November 1980https://pubs.acs.org/doi/10.1021/ja00543a011https://doi.org/10.1021/ja00543a011research-articleACS PublicationsRequest reuse permissionsArticle Views63Altmetric-Citations11LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Genetic Heterogeneity in Therapy-Naïve Synchronous Primary Breast Cancers and Their Metastases (2017)

<b>Purpose:</b> Paired primary breast cancers and metachronous metastases after adjuvant treatment are reported to differ in their clonal composition and genetic alterations, but it is unclear whether these differences stem from the selective pressures of the metastatic process, the systemic therapies, or both. We sought to define the repertoire of genetic alterations in breast cancer patients with <i>de novo</i> metastatic disease who had not received local or systemic therapy.<b>Experimental Design:</b> Up to two anatomically distinct core biopsies of primary breast cancers and synchronous distant metastases from nine patients who presented with metastatic disease were subjected to high-depth whole-exome sequencing. Mutations, copy number alterations and their cancer cell fractions, and mutation signatures were defined using state-of-the-art bioinformatics methods. All mutations identified were validated with orthogonal methods.<b>Results:</b> Genomic differences were observed between primary and metastatic deposits, with a median of 60% (range 6%-95%) of shared somatic mutations. Although mutations in known driver genes including <i>TP53, PIK3CA</i>, and <i>GATA3</i> were preferentially clonal in both sites, primary breast cancers and their synchronous metastases displayed spatial intratumor heterogeneity. Likely pathogenic mutations affecting epithelial-to-mesenchymal transition-related genes, including <i>SMAD4, TCF7L2</i>, and <i>TCF4</i> (<i>ITF2</i>), were found to be restricted to or enriched in the metastatic lesions. Mutational signatures of trunk mutations differed from those of mutations enriched in the primary tumor or the metastasis in six cases.<b>Conclusions:</b> Synchronous primary breast cancers and metastases differ in their repertoire of somatic genetic alterations even in the absence of systemic therapy. Mutational signature shifts might contribute to spatial intratumor genetic heterogeneity. <i>Clin Cancer Res; 23(15); 4402-15. ©2017 AACR</i>.