gebhart/patrepeval huggingface dataset:
Formate Dehydrogenase Localization and Activity Are Dependent on an Intact Twin Arginine Translocation System (Tat) in <i>Campylobacter jejuni</i> 81-176 (2014)

The oxidation of formate constitutes a main energy source for Campylobacter jejuni, an important foodborne pathogen. Formate occurs in the hosts' gut as a byproduct of fermentation, which may contribute to C. jejuni's adaptation to that niche. C. jejuni possesses a periplasmic formate dehydrogenase (Fdh) that facilitates the metabolism of formate. In C. jejuni NCTC-11168, a poorly invasive strain, Fdh localizes to the periplasm via the twin arginine translocation (Tat) system, and the fdhA encodes the signature Tat signal motif. However, screening the genome of C. jejuni 81-176, a highly invasive strain, showed that the Tat signal motif was encoded on a separate gene (CJJ81176_1504) directly upstream of the fdhA (CJJ81176_1503). This possibly suggested a difference in formate metabolism between these two strains. Therefore, we investigated whether the Fdh was Tat dependent in C. jejuni 81-176. For this purpose, we measured formate respiration in C. jejuni 81-176 (wild-type), a Tat mutant (ΔtatC-81-176), and a complementation strain (C-ΔtatC-81-176) using BIOLOG-AN plates. We also used a viologen-based assay to specifically measure the Fdh enzymatic activity in whole cells and periplasmic preparations of the wild-type and mutant strains. Collectively, our data indicated that the Fdh activity and localization in C. jejuni 81-176 were dependent on a functional Tat system. The proper maturation and maintenance of Fdh are essential for cognate energy production, which might explain the association of the Fdh with the Tat system across different strains. The latter highlights the potential of the Tat system and Fdh as appealing targets for novel anti-C. jejuni therapeutics.
Holographic three-dimensional printer: new method (1992)

We propose a holographic three-dimensional (3-D) printer that produces 3-D hard copies of computer data. A new technique for synthesizing a holographic stereogram (HS) has been invented for the purpose of making accurate hard copies of 3-D objects. The flat-format, Lippmann-type HS is printed by one optical step and is ideally suitable for 3-D printer application. This type of HS has both horizontal and vertical parallaxes so that the reconstructed image is free from distortion. The principle and the image quality of this type of HS are described, and a reconstructed image from this type of HS with 160 x 128 elements is experimentally demonstrated. The systematic constitution of a holographic 3-D printer is also discussed.
Reactions of Methyl Fluoride with Atomic Transition-Metal and Main-Group Cations:  Gas-Phase Room-Temperature Kinetics and Periodicities in Reactivity (2006)

Reactions of CH(3)F have been surveyed systematically at room temperature with 46 different atomic cations using an inductively coupled plasma/selected-ion flow tube tandem mass spectrometer. Rate coefficients and product distributions were measured for the reactions of fourth-period atomic ions from K(+) to Se(+), of fifth-period atomic ions from Rb(+) to Te(+) (excluding Tc(+)), and of sixth-period atomic ions from Cs(+) to Bi(+). Primary reaction channels were observed corresponding to F atom transfer, CH(3)F addition, HF elimination, and H(2) elimination. The early-transition-metal cations exhibit a much more active chemistry than the late-transition-metal cations, and there are periodic features in the chemical activity and reaction efficiency that maximize with Ti(+), As(+), Y(+), Hf(+), and Pt(+). F atom transfer appears to be thermodynamically controlled, although a periodic variation in efficiency is observed within the early-transition-metal cations which maximizes with Ti(+), Y(+), and Hf(+). Addition of CH(3)F was observed exclusively (>99%) with the late-fourth-period cations from Mn(+) to Ga(+), the fifth-period cations from Ru(+) to Te(+), and the sixth-period cations from Hg(+) to Bi(+) as well as Re(+). Periodic trends are observed in the effective bimolecular rate coefficient for CH(3)F addition, and these are consistent with expected trends in the electrostatic binding energies of the adduct ions and measured trends in the standard free energy of addition. HF elimination is the major reaction channel with As(+), while dehydrogenation dominates the reactions of W(+), Os(+), Ir(+), and Pt(+). Sequential F atom transfer is observed with the early-transition-metal cations, with the number of F atoms transferred increasing across the periodic table from two to four, maximizing at four for the group 5 cations Nb(+)(d(4)) and Ta(+)(d(3)s(1)), and stopping at two with V(+)(d(4)). Sequential CH(3)F addition was observed with many atomic cations and all of the metal mono- and multifluoride cations that were formed.
Design of optimal process parameters for non‐isothermal forging (1994)

Abstract This paper presents a state space model and an optimal design scheme for non‐isothermal metal forming processes. By selecting nodal velocity and temperature as the state variables, a non‐isothermal state equation with coupled deformation and thermal terms is established. Based on this state space model, a control design scheme is developed to obtain the optimal die velocity and initial die temperature which will ensure that the effective strain‐rate and temperature satisfy the design requirements. A titanium alloy engine disk forging is used to demonstrate two design examples. The results show that the proposed model and design scheme behave well for different design requirements.
Foreword (2019)

It is our great pleasure as Editor and Deputy Editor-in-Chief of the Journal of Head and Neck Anesthesia (JoHNA) to introduce you to the inaugural online issue of the Journal, presented in partnership between the Society for Head and Neck Anesthesia and Lippincott Williams & Wilkins (LWW) division of Wolters Kluwer Health. The Society for Head and Neck Anesthesia (SHANA, www.shanahq.com) is an award-winning, leading information resource in the rapidly expanding field of Head and Neck Anesthesia, and has over 600 members worldwide. LWW is a leading international health care publisher, with nearly 300 periodicals and 1500 books in more than 100 disciplines. Its extensive anesthesiology journal portfolio includes the renowned publications, such as Anesthesiology, Anesthesia and Analgesia, International Anesthesiology Clinics, European Journal of Anaesthesiology, Current Opinion in Anesthesiology, and others. The SHANA-LWW partnership represents an important advancement for JoHNA and the field of Head and Neck Anesthesia. The Journal will offer open access to publications important to our field, with the reprint of all prior editions of the Journal previously available on the web (www.shanahq.com). We have secured an editorial board comprising some of the leading experts in the field, to support JoHNA’s high-quality clinical and research publications. As the Journal develops with such a reputable publisher, we also anticipate accelerated indexing of JoHNA across the common databases for further dissemination of our work. We encourage submission of your important work to advance the field of Head and Neck Anesthesia for the benefit of the international anesthesia community. Submissions should be sent to the online editorial manager system, www.editorialmanager.com/johna/default.aspx. Clinical studies stand to answer important questions to advance the safe care of patients undergoing head and neck surgeries. For SHANA members, the selected manuscripts will have article-processing charges waived or discounted, depending on their submission. Manuscripts will be handled by the editorial board with a robust peer review process. The editorial management system will serve as an important tool to streamline the process, offer early decision-making, and provide manuscript-tracking features. Thank you for your interest in the Journal of Head and Neck Anesthesia. We look forward to working with you to disseminate your important contributions to the field. Conflict of interest disclosures The authors declare that they have no financial conflict of interest with regard to the content of this report.
α-Synuclein accumulation and GBA deficiency due to L444P GBA mutation contributes to MPTP-induced parkinsonism (2018)

Our results suggest that GBA deficiency due to L444P GBA heterozygous mutation and the accompanying accumulation of α-synuclein render DA neurons more susceptible to MPTP intoxication. Thus, GBA and α-synuclein play dual physiological roles in the survival of DA neurons in response to the mitochondrial dopaminergic neurotoxin, MPTP.
Case Studies--Offshore Managed Pressure Drilling (2006)

Managed Pressure Drilling (MPD) technology has evolved on land drilling programs since the mid-sixties. However, it has been only fairly recently that the technology has found an important niche in the minds of offshore drilling decision-makers.With most of the world's remaining prospects for oil and gas being economically un-drillable with conventional-wisdom casing set points and fluids programs, MPD is now being viewed as a technology which can render a significant increase in economic drill-ability by reducing excessive drilling-related costs typically associated with conventional offshore drilling.This paper will define for the audience the two Categories (Reactive & Proactive) and the Variations of MPD that are being currently practiced or will be practiced in the near future in marine environments:CBHP (Constant Bottomhole Pressure)PMCD (Pressurized Mud Cap Drilling)Dual Gradient (several methods)HSE (Returns Flow Control)Case studies will be presented which speak to MPD applications from both fixed (Jackup, platform) rigs with surface BOP stacks…and from floating rigs (moored semi-submersibles and DP Drillships) with subsea and surface BOP's.There will be an emphasis upon the
Supplementary Figures 1-8 from Patient-Derived iPSCs Faithfully Represent the Genetic Diversity and Cellular Architecture of Human Acute Myeloid Leukemia (2023)

&lt;p&gt;Supplemental Figure 1. Generation of a panel of iPSCs from patients with AML. Supplemental Figure 2. Reprogramming aids reconstruction of the evolutionary history and clonal composition of AML. Supplemental Figure 3. Transplantation of AML-iPSCs into immunodeficient mice. Supplemental Figure 4. Developmental block in a subset of AML-iPSC lines. Supplemental Figure 5. Transplantation of primary AML cells and patient-matched AMLiPSC lines. Supplemental Figure 6. Single-cell RNA-sequencing analyses of matched primary and iPSC-derived leukemia cells from patient AML-47. Supplemental Figure 7. Cell cycle and pseudotime analyses. Supplemental Figure 8. Comparison of scRNA-Seq data integration and clustering methods and pseudobulk differential gene expression analyses.&lt;/p&gt;
Progenitor Cells in Infantile Hemangioma (2009)

Infantile hemangioma is a vascular tumor that occurs in 5% to 10% of infants of European descent. A hallmark of infantile hemangioma is its life cycle, which is divided into 3 stages. The proliferating phase spans in the first year of postnatal life and is characterized by cellular masses without a defined vascular architecture and nascent blood vessels with red blood cells evident within the lumenal space. The involuting phase begins at 1 year of age and continues for 3 to 5 years. Proliferation slows or stops in this phase, and histologic examination shows that the blood vessel architecture becomes more obvious and vessel size is enlarged. The involuted phase reaches 5 to 8 years of age, at which point blood vessels are replaced with a fibrofatty residuum and capillary-sized channels. The growth and involution life cycle of infantile hemangioma are very different from other vascular tumors and vascular malformations, which do not regress and can occur at any time during childhood or adult life. Many laboratories have reported on the endothelial characteristics of the cellular masses that are prominent in the proliferating phase of infantile hemangioma, as well as their immature appearance. These studies, along with isolation and characterization of hemangioma-derived cell populations with progenitor cell properties, have lead to an emerging hypothesis that hemangioma is caused by an abnormal or delayed differentiation of mesodermal progenitor cells into the disorganized mass of blood vessels. In this paper, we discuss the literature that supports this emerging hypothesis.
A New THP Precoding Scheme with Effective Channel Optimization (2009)

Recently there is a trend to use Tomlinson-Harashima preceding (THP) to achieve better performance with full channel state information known at transmit side. In general, performance of THP precoding is governed by the the minimum absolute diagonal elements of the triangle matrix which is derived from QR decomposition to the channel matrix. In this paper we propose a new THP precoding scheme in which codebook based precoding is applied to optimize the effective channel after THP precoding with the aim of maximizing the minimum absolute diagonal elements of the triangle matrix. As a result, about 4 to 8 dB gains are obtained by employing codebooks of different sizes. The merits of the proposed scheme are low complexity, flexible tradeoff between complexity and performance by employing codebook with different sizes, and friendly to hardware implementation because of its fixed complexity. The theory analysis on the BER performance of THP precoding scheme is provided in this paper which is close to the simulation results. Numerical simulation results verify our conclusions.
VWF protects FVIII from endocytosis by dendritic cells and subsequent presentation to immune effectors (2006)

Von Willebrand factor (VWF) is a chaperone molecule for procoagulant factor VIII (FVIII). Its role in the reduction of the immunogenicity of therapeutic FVIII in patients with hemophilia A has been evoked but lacks clear cellular and molecular rationale. Here, we demonstrate that VWF protects FVIII from being endocytosed by human dendritic cells (DCs) and subsequently presented to FVIII-specific T cells. The immunoprotective effect of VWF requires a physical interaction with FVIII because the endocytosis of FVIII was significantly restored on hindering the formation of the VWF-FVIII complex. Interestingly, VWF had no direct inhibitory effect either on the ability of DCs to present antigenic peptides or on the activation potency of CD4+ T cells. We thus propose that VWF may reduce the immunogenicity of FVIII by preventing, upstream from the activation of immune effectors, the entry of FVIII in professional antigen-presenting cells.
miR-155 Is Essential for Inflammation-Induced Hippocampal Neurogenic Dysfunction (2015)

Peripheral and CNS inflammation leads to aberrations in developmental and postnatal neurogenesis, yet little is known about the mechanism linking inflammation to neurogenic abnormalities. Specific miRs regulate peripheral and CNS inflammatory responses. miR-155 is the most significantly upregulated miR in primary murine microglia stimulated with lipopolysaccharide (LPS), a proinflammatory Toll-Like Receptor 4 ligand. Here, we demonstrate that miR-155 is essential for robust IL6 gene induction in microglia under LPS stimulation in vitro. LPS-stimulated microglia enhance astrogliogenesis of cocultured neural stem cells (NSCs), whereas blockade of IL6 or genetic ablation of microglial miR-155 restores neural differentiation. miR-155 knock-out mice show reversal of LPS-induced neurogenic deficits and microglial activation in vivo. Moreover, mice with transgenic elevated expression of miR-155 in nestin-positive neural and hematopoietic stem cells, including microglia, show increased cell proliferation and ectopically localized doublecortin-positive immature neurons and radial glia-like cells in the hippocampal dentate gyrus (DG) granular cell layer. Microglia have proliferative and neurogenic effects on NSCs, which are significantly altered by microglial miR-155 overexpression. In addition, miR-155 elevation leads to increased microglial numbers and amoeboid morphology in the DG. Our study demonstrates that miR-155 is essential for inflammation-induced neurogenic deficits via microglial activation and induction of IL6 and is sufficient for disrupting normal hippocampal development.
Folding correction of ABC-transporter ABCB1 by pharmacological chaperones: a mechanistic concept (2017)

Point mutations of ATP-binding cassette (ABC) proteins are a common cause of human diseases. Available crystal structures indicate a similarity in the architecture of several members of this protein family. Their molecular architecture makes these proteins vulnerable to mutation, when critical structural elements are affected. The latter preferentially involve the two transmembrane domain (TMD)/nucleotide-binding domain (NBD) interfaces (transmission interfaces), formation of which requires engagement of coupling helices of intracellular loops with NBDs. Both, formation of the active sites and engagement of the coupling helices, are contingent on correct positioning of ICLs 2 and 4 and thus an important prerequisite for proper folding. Here, we show that active site compounds are capable of rescuing P-glycoprotein (P-gp) mutants ∆Y490 and ∆Y1133 in a concentration-dependent manner. These trafficking deficient mutations are located at the transmission interface in pseudosymmetric position to each other. In addition, the ability of propafenone analogs to correct folding correlates with their ability to inhibit transport of model substrates. This finding indicates that folding correction and transport inhibition by propafenone analogs are brought about by binding to the active sites. Furthermore, this study demonstrates an asymmetry in folding correction with cis-flupentixol, which reflects the asymmetric binding properties of this modulator to P-gp. Our results suggest a mechanistic model for corrector action in a model ABC transporter based on insights into the molecular architecture of these transporters.
Direct .beta.-lithiation of 2-alkoxy dienes: use in an asymmetric Diels-Alder reaction (1986)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDirect .beta.-lithiation of 2-alkoxy dienes: use in an asymmetric Diels-Alder reactionPatrick G. McDougal, Joseph G. Rico, and Don VanDerveerCite this: J. Org. Chem. 1986, 51, 23, 4492–4494Publication Date (Print):November 1, 1986Publication History Published online1 May 2002Published inissue 1 November 1986https://pubs.acs.org/doi/10.1021/jo00373a035https://doi.org/10.1021/jo00373a035research-articleACS PublicationsRequest reuse permissionsArticle Views241Altmetric-Citations30LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
KIFC1 as a novel therapeutic target for p53 mutant colorectal cancer. (2018)

e15585 Background: Loss or mutation of p53 gene is the most frequent genetic lesion in late-stage colon cancers. The optimal management of p53 mutant (p53M) colorectal cancer (CRC) remains a therapeutic challenge owing to its resistance to 5-fluorouracil, the first-choice chemotherapy drug for CRC. Thus, the search for novel therapeutic targets for the treatment of p53M CRC is highly warranted. We report that p53 negatively regulates expression of KIFC1 (a centrosome clustering protein), can serve as a potential therapeutic target for p53M CRCs. Methods: We first immunohistochemically stained 203 CRC tissue samples for p53, KIFC1, and FOXM1 and calculated their weighted indices (WIs) for nuclear staining. To validate the clinical findings in silico analysis was performed on the publically available dataset (GSE41258). For determining the mechanistic relationship of KIFC1 and p53, overexpression (OE) of p53 and immunoblotting and ChiP assays were performed. Finally, inhibited KIFC1 by pharmacological and genetic methods to elucidate the role of the KIFC1 inhibition on the vitality of p53-/- CRC cells. Results: p53-/- and p53M CRC [p53-/- n = 82, p53M n = 40 and WT n = 81] exhibited significantly (p < 0.001) higher expression of KIFC1 and FOXM1 when compared with the p53 WT CRC tissue samples and was associated with worse overall survival (HR = 4.249, p = < 0.001)). Similarly, our in silico analysis indicated significantly higher KIFC1 and FOXM1 mRNA expression in p53M (n = 93) CRC and was associated with poor overall survival (HR = 1.46, P = < 0.05). OE of p53 in p53-/- cells decreased the expression of FOXM1 and KIFC1 indicating the negative regulation of KIFC1 via FOXM1 which was further strengthened by ChiP assay (FOXM1 interaction with KIFC1 promoter was significantly higher p = 0.037 in p53-/- cells when compared with p53 WT cells). Inhibition of KIFC1 using shRNA, CW069 (a known KIFC1 inhibitor) and griseofulvin (a known centrosome declustering drug), led to increased multipolarity followed by cell death only in p53-/- CRC cells. Conclusions: Collectively our results indicate that p53 negatively regulates expression of KIFC1 via FOXM1 and contributes to poor outcomes in p53M CRCs. Thus, KIFC1 may serve as a potential therapeutic target for p53M CRCs.
Essential oil composition of three accessions of<i>Dracocephalum heterophyllum</i>Benth. cultivated at Palampur, India (2008)

Seeds of three accessions of Dracocephalum heterophyllum growing wild in high altitude locations in the cold desert regions of trans-Himalaya were collected and propagated under polyhouse conditions at IHBT Palampur to evaluate, compare their growth performance and essential oil composition. Three different chemotypes viz., citronellol-rose oxides type, citronellol type and citronellal-trans rose oxide type were identified. Samples of essential oils were distilled separately from leaves and inflorescence of all the three cultivated accessions and characterised by capillary GC and GC-MS techniques, which showed variation in the volatile constituents. In all the three accessions, citronellol was higher in the inflorescence oils as compared to leaf oils. Rose oxides, which are important high odour value cyclic monoterpene ethers were found in the essential oil samples of leaves as well as in the inflorescence of accession II, whereas these constituents were absent in the leaves of other two accessions. The essential oil from the inflorescence part of accession I was devoid of both cis and trans isomers of rose oxide, while accession III contained only one isomer of trans rose oxide. Under polyhouse conditions, seed germination was higher in accession III, whereas aerial biomass was higher in accession I. In comparison to other accessions, yield of volatile oil was higher in both leaf and inflorescence of accessions I, whereas accession II had higher oil yield from inflorescence. This is the first report of volatile oil composition of three accessions of D. heterophyllum cultivated at Palampur having potential as a new source of high valued essential oil for introduction in the perfumery industry.