gebhart/patrepeval huggingface dataset:
Transcriptome of pancreas-specific<i>Bmpr1a</i>-deleted islets links to TPH1–5-HT axis (2015)

Bone morphogenetic protein (BMP) signaling is crucial for the development and function of numerous organs, but its role on the function of pancreatic islets is not completely clear. To explore this question, we applied the high throughput transcriptomic analyses on the islets isolated from mice with a pancreas-specific deletion of the gene, Bmpr1a, encoding the type 1a BMP receptor. Consistently, these pBmpr1aKO mice had impaired glucose homeostasis at 3 months, and were more severely affected at 12 months of age. These had lower fasting blood insulin concentrations, with reduced expression of several key regulators of β-cell function. Importantly, transcriptomic profiling of 3-month pBmpr1aKO islets and bioinformatic analyses revealed abnormal expression of 203 metabolic genes. Critically among these, the tryptophan hydroxylase 1 gene (Tph1), encoding the rate-limiting enzyme for the production of 5-hydroxytryptamine (5-HT) was the highest over-expressed one. 5-HT is an important regulator of insulin secretion from β cells. Treatment with excess 5-HT inhibited this secretion. Thus our transcriptomic analysis links two highly conserved molecular pathways the BMP signaling and the TPH1-5-HT axis on glucose homeostasis.
Enhanced charge-carrier injection caused by molecular orientation (2011)

Current densities of hole-only devices based on alpha-sexithiophene (α-6T) and N-N′-diphenyl-N-N′-bis(1-naphthyl)-1,1′-biphenyl-4,4′-diamine (α-NPD) increase 42 times at 1 V by rubbing α-6T with a Nylon cloth. Results of absorption spectroscopy with normal and oblique light incidence reveal that the rubbing induces a change from standing to lying orientations of α-6T in a film surface region. The increased current densities are attributed to enhanced α-6T-to-α-NPD hole injection due to the lying orientation.
Airway Reactivity Changes in Asthmatic Patients Undergoing Blinded Food Challenges (1996)

To investigate the possible pathogenic role of food allergy in asthma, airway hyperresponsiveness was measured by methacholine inhalation challenges (MIC) performed before and after double-blind, placebo-controlled food challenges (DBPCFC) in 26 food-allergic, asthmatic patients. Airway hyperresponsiveness was classified as severe in two cases (PD20FEV1 < 2 breath units, BU), moderate in 18 (PD20FEV1: 2-20 BU), and mild in six (PD20FEV1 > 20 BU). Medications included albuterol (81%), inhaled steroids (38%), cromolyn (35%), and theophylline (23%). MICs were performed in the afternoon after DBPCFC. Of the 22 positive DBPCFC, 12 involved chest symptoms (cough, wheezing, or both). Another 10 positive DBPCFCs included laryngeal, gastrointestinal, and/or skin symptoms without any chest symptoms. Significant increases in airway hyperresponsiveness were evident in seven of 12 patients experiencing chest symptoms during DBPCFC. Significant increases in airway hyperresponsiveness were observed in one patient without chest symptoms during a positive DBPCFC and one patient after a negative DBPCFC. However, this last patient had a negative MIC with the same antigen 1 yr later. These studies indicate that food-induced allergic reactions can increase airway reactivity, and may do so without inducing acute asthma.
Fully biobased triblock copolymers generated using an unconventional oscillatory plug flow reactor (2022)

Producing block polymers in continuous flow offers significant advantages in terms of versatility, efficiency and scalability.
4H-SiC-DIMOSFET power device for home appliances (2010)

The 5×4.2 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> chip of the SiC DIMOSFET was fabricated and tested. The drain-source avalanche breakdown voltage without any gate bias (Vgs=0V) is measured to be >1000V. The drain current (Id) >40A is observe under the conditions of Vds=1V and Vgs=+20V. Typical Ron and specific Ron are measured to be 22 mΩ and 3.5 mΩcm2 with V <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">th</sub> =2.3V. The SiC DIMOSFET is introduced into the PE circuits of the commercially available all-metal IH cooktop to test the loss-energy saving. On the PFC circuit, 2/3 reduction of the energy loss of the SW device is confirmed. On the inverter circuit, 21W reduction of the energy loss of the SW device is confirmed.
ChemInform Abstract: Lead Optimization of the N1 Substituent of a Novel Series of Indazole Arylsulfonamides as CCR4 Antagonists and Identification of a Candidate for Clinical Investigation. (2012)

Abstract Compound (Id) is identified for further development within a novel series of indazole sulfonamide chemokine receptor 4 (CCR4) antagonists.
Effects of daily porcine somatotropin administration on the lysine requirement of growing pigs (1991)

One-hundred twenty crossbred barrows initially weighing 68.7 lb were used to determine the lysine requirement of growing pigs injected with 5 mg/d porcine somatotropin (PST) during a 5-wk growth trial. Pigs received one of six levels of dietary lysine (.7, 1.1, 1.5, 1.9,2.3, or 2.7%) and were injected daily with either 5 mg pST or placebo. During the 5 wk trial, pST-injected pigs had increased average daily gain (ADG), decreased average daily feed intake (ADFI), and improved feed conversion (F/G) compared with placebo-injected pigs. With increasing dietary lysine, ADFI of pigs injected with both pST and placebo was reduced. Pigs injected with pST had improved ADG as dietary lysine increased to 1.5% and improved F/G as dietary lysine increased to 1.9 %. Dietary lysine had no effect on ADG or F/G of placebo-injected pigs. Pigs injected with pST had improved average backfat, tenth rib fat depth, and longissimus area compared to placebo-injected pigs. Tenth rib fat depth of both placebo- and pST-injected pigs was reduced as dietary lysine increased. Longissimus area of pST-injected pigs improved as dietary lysine increased to 1.5%. The improvements in ADG, ADFI, F/G, and longissimus area of pST-injected pigs indicate that the dietary lysine requirement of growing pigs injected with 5 mg/d pST is 1.5 to 1.9%.; Swine Day, Manhattan, KS, November 21. 1991
Analysis of Electric-Submersible-Pumping Systems (1989)

Summary This paper presents a field-proven analytical method of evaluating electric-submersible-pumping equipment and well performance jointly. A computerized mathematical model that considers the effects of free gas, pump speed (variable-frequency drives), and pump tapering is described. The method allows accurate calculations of important downhole parameters, including pump intake pressure, pump intake volume (including free gas), pump pressure, and fluid density profile. Lifting cost parameters—such as pump and motor power requirements, monthly power consumption, pump and motor performance, and overall system efficiency—are also determined. Once operating conditions are known, decisions can be made on ways to increase production (if additional potential exists) or to reduce operating costs. Thus, the same basic goals that often justify frequent analysis of rod-pumping systems can be applied to submersible pumping.
Auto-Discovery VPN Problem Statement and Requirements (2013)

This document describes the problem of enabling a large number of systems to communicate directly using IPsec to protect the traffic between them. It then expands on the requirements for such a solution.
Combined myofibrillar and mitochondrial creatine kinase deficiency impairs mouse diaphragm isotonic function (1997)

Creatine kinase (CK) is an enzyme central to cellular high-energy phosphate metabolism in muscle. To characterize the physiological role of CK in respiratory muscle during dynamic contractions, we compared the force-velocity relationships, power, and work output characteristics of the diaphragm (Dia) from mice with combined myofibrillar and sarcomeric mitochondrial CK deficiency (CK[-/-]) with CK-sufficient controls (Ctl). Maximum velocity of shortening was significantly lower in CK[-/-] Dia (14.1 +/- 0.9 Lo/s, where Lo is optimal fiber length) compared with Ctl Dia (17.5 +/- 1.1 Lo/s) (P < 0.01). Maximum power was obtained at 0.4-0.5 tetanic force in both groups; absolute maximum power (2,293 +/- 138 W/m2) and work (201 +/- 9 J/m2) were lower in CK[-/-] Dia compared with Ctl Dia (2,744 +/- 146 W/m2 and 284 +/- 26 J/m2, respectively) (P < 0.05). The ability of CK[-/-] Dia to sustain shortening during repetitive isotonic activation (75 Hz, 330-ms duration repeated each second at 0.4 tetanic force load) was markedly impaired, with CK[-/-] Dia power and work declining to zero by 37 +/- 4 s, compared with 61 +/- 5 s in Ctl Dia. We conclude that combined myofibrillar and sarcomeric mitochondrial CK deficiency profoundly impairs Dia power and work output, underscoring the functional importance of CK during dynamic contractions in skeletal muscle.
An Integrated In Silico, In Vitro and Tumor Tissues Study Identified Selenoprotein S (SELENOS) and Valosin-Containing Protein (VCP/p97) as Novel Potential Associated Prognostic Biomarkers in Triple Negative Breast Cancer (2022)

Combined evaluation of SELENOS and VCP/p97 might represent a novel potential prognostic signature and a therapeutic target to be exploited in TNBC.
Physical layer performance of optical packet switches: A practical approach (2011)

Simulation results of the OSNR performance of an optical switch/router are presented in this paper. The paper is completed with a cascadability analysis, which takes into account the optical link that joins the nodes, and some results that show the effects of the scheduling algorithm on the optical performance.
Gate-All-Around Buckled Dual Si Nanowire nMOSFETs on Bulk Si for Transport Enhancement and Digital Logic Application (2012)

Keywords: Gate-all-around Si nanowire MOSFET ; Uniaxial tensile stress ; Vertical stack of nanowires ; Micro-Raman spectroscopy ; Optical/electrical characterization methods in nanoscale ; Inversion-mode ; Mutli-gate logic on bulk Si Reference EPFL-CONF-179920 URL: http://www.mne12.org/en/?page_id=964 Record created on 2012-07-10, modified on 2016-08-09
Marizomib activity as a single agent in malignant gliomas: ability to cross the blood-brain barrier (2015)

These preclinical studies demonstrated that marizomib can cross the blood-brain barrier and inhibit proteasome activity in rodent and nonhuman primate brain and elicit a significant antitumor effect in a rodent intracranial model of malignant glioma.
HUMAN AMNIOTIC MEMBRANE FOR NOVEL APPLICATIONS IN REGENERATIVE MEDICINE (2018)

The human amniotic membrane (hAM) contains cells of stem cell characteristics with low immunogenicity and anti-inflammatory properties and has for centuries been applied in the clinics especially f...
Development and validation of a broad scheme for prediction of HLA class II restricted T cell epitopes (2014)

Computational prediction of HLA class II restricted T cell epitopes has great significance in many immunological studies including vaccine discovery. With the development of novel bioinformatics approaches, prediction of HLA class II binding has improved significantly but a strategy to predict the most dominant HLA class II epitopes has not been defined. Using different sets of peptides from various allergen and bacterial antigens and HLA class II binding prediction tools from the IEDB, we have designed a strategy to predict the top epitopes from any antigen. We found that the top 21% of 15-mer peptides overlapping by 10 residues (based on the predicted binding to seven DRB1 and DRB3/4/5 alleles) capture 50% of the immune response. This corresponded to an IEDB consensus percentile rank of 19.82 which could be used as a universal prediction threshold.
Palladium-promoted Coupling Reactions of [11C]Methyl Iodide with Organotin and Organoboron Compounds. (1995)

Methods for the rapid incorporation of C-11 (beta(+), t1/2 = 20.3 min) into a number of compounds have been developed. By the use of palladium-promoted cross-coupling of [C-11]methyl iodide with organostannanes, the model compounds [alpha-C-11]toluene, m
Connections between respiratory neurones in the neonatal rat transverse medullary slice studied with cross‐correlation (2003)

In the transverse medullary slice prepared from neonatal rats the hypoglossal nerve rootlets exhibit a bursting 'respiratory' rhythm as do neurones in the pre-Bötzinger complex (PBC). We used cross-correlation analysis of the rhythmic multiunit discharges recorded from hypoglossal nerve rootlets, hypoglossal nucleus neurones and PBC neurones to investigate the connections between these groups. All cross-correlograms computed between left and right hypoglossal nerves, and between hypoglossal neurones and contralateral hypoglossal nerves, displayed central peaks with broad half-amplitude widths (mean +/- S.D. of 29.6 +/- 10.4 and 37.3 +/- 6.0 ms, respectively), which we interpreted as evidence for activation from a common source. Five of the 18 cross-correlograms computed between left and right PBC neurones displayed peaks either side of time zero with narrower half-amplitude widths (mean +/- S.D. of 9.3 +/- 1.9 ms) superimposed on broader central peaks, which we interpreted as evidence for mutual excitation and common activation, respectively. Cross-correlograms computed between PBC neurones and contralateral hypoglossal neurones or nerves did not display consistent features, but some of those computed between PBC and ipsilateral hypoglossal neurones (two of eight) or nerves (two of five) displayed peaks with broad half-amplitude widths (mean +/- S.D. of 36.8 +/- 6.9 ms), offset from time zero by 6 ms (except for one at 18 ms), which we interpreted as evidence for excitation of hypoglossal neurones and motoneurones by PBC neurones. We concluded that rhythm is synchronised between left and right sides by mutual excitatory connections between left and right PBC neurones. The rhythm is transmitted to ipsilateral hypoglossal neurones by a paucisynaptic pathway. Both hypoglossal neurones and PBC neurones receive a common activation from as yet unidentified sources.
Bioconjugatable Azo‐Based Dark‐Quencher Dyes: Synthesis and Application to Protease‐Activatable Far‐Red Fluorescent Probes (2012)

We describe the efficient synthesis and one-step derivatization of novel, nonfluorescent azo dyes based on the Black Hole Quencher-3 (BHQ-3) scaffold. These dyes were equipped with various reactive and/or bioconjugatable groups (azido, α-iodoacetyl, ketone, terminal alkyne, vicinal diol). The azido derivative was found to be highly reactive in the context of copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions and allowed easy synthetic access to the first water-soluble (sulfonated derivative) and aldehyde-modified BHQ-3 dyes, the direct preparation of which failed by means of conventional azo-coupling reactions. The aldehyde- and α-iodoacetyl-containing fluorescence quenchers were readily conjugated to aminooxy- and cysteine-containing peptides by the formation of a stable oxime or thioether linkage, respectively. Further fluorescent labeling of the resultant peptide conjugates with red- or far-red-emitting rhodamine or cyanine dyes through sequential and/or one-pot bioconjugations, led to novel Förster resonance energy transfer (FRET) based probes suitable for the in vivo detection and imaging of urokinase plasminogen activator, a key protease in cancer invasion and metastasis.
Enhancement of +1 Frameshift by Polyamines during Translation of Polypeptide Release Factor 2 in Escherichia coli (2006)

Polypeptide release factor 2 (RF2) in Escherichia coli is known to be synthesized by a +1 frameshift at the 26th UGA codon of RF2 mRNA. Polyamines were found to stimulate the +1 frameshift of RF2 synthesis, an effect that was reduced by excess RF2. Polyamine stimulation of +1 frameshift of RF2 synthesis was observed at the early logarithmic phase, which is the important phase in determination of the overall rate of cell growth. A Shine-Dalgarno-like sequence was necessary for an efficient +1 frameshift of RF2 synthesis, but not for polyamine stimulation. Spectinomycin, tetracycline, streptomycin, and neomycin reduced polyamine stimulation of the +1 frameshift of RF2 synthesis. The results suggest that a structural change of the A site on 30 S ribosomal subunits is important for polyamine stimulation of the +1 frameshift. The level of mRNAs of ribosomal proteins and elongation factors having UAA as termination codon was enhanced by polyamines, and OppA synthesis from OppA mRNA having UAA as termination codon was more enhanced by polyamines than that from OppA mRNA having a UGA termination codon. Furthermore, synthesis of ribosomal protein L20 and elongation factor G from the mRNAs having a UAA termination codon was enhanced by polyamines at the level of translation and transcription. The results suggest that some protein synthesis from mRNAs having a UAA termination codon is enhanced at the level of translation through polyamine stimulation of +1 frameshift of RF2 synthesis. It is concluded that prfB encoding RF2 is a new member of the polyamine modulon.
Enhanced myocardial salvage by combined treatment with recombinant single-chain urokinase-type plasminogen activator and recombinant human superoxide dismutase in a canine coronary thrombosis model. (1988)

Myocardial ischemia was induced by formation of an occlusive thrombus in the left anterior circumflex artery (LCX) in open chest dogs. The myocardial salvage by the fibrin specific recombinant single-chain urokinase-type plasminogen activator (r-scu-PA) alone and in combination with the oxygen radical scavenger human superoxide dismutase of recombinant origin (r-HSOD) was investigated. The three experimental groups were: group I (n = 4) did not receive any treatment after LCX thrombosis; in group II (n = 9) at 100 min after LCX thrombosis r-scu-PA (20 micrograms.kg1.min-1 i.v. for 30 min) was infused; dogs in group III (n = 8) received concomitant treatment with r-scu-PA and r-HSOD (10 mg.kg1 i.v. for 60 min). Percent of left ventricle at risk did not differ between the groups. Infarct size as percent of the risk zone was 45.3 +/- 8.0 in group I, 25.3 +/- 3.7 in group II (a less than or equal to 0.05 vs group I) and 14.9 +/- 3.2 in group III (a less than 0.05 vs group II). Incidence of reperfusion arrhythmias and increase in plasma creatine kinase were significantly diminished by r-HSOD when compared to dogs receiving r-scu-PA only. There were no significant differences in hemodynamic parameters between the groups. No changes in plasma fibrinogen were observed in r-scu-PA treated dogs. In conclusion, the combined treatment with r-scu-PA and r-HSOD yielded a significantly higher myocardial salvage versus thrombolytic treatment alone in a canine LCX thrombosis model.
Aging Phenotypes and Restoring Functional Deficits in Older Adults With Hematologic Malignancy (2021)

The OEP was effective in improving quality of life, and geriatric tools predicted survival and hospital utilization among older adults with hematologic malignancies.
Impact of lactic acid on cell proliferation and free radical‐induced cell death in monolayer cultures of neural precursor cells (2009)

Biomaterials prepared from polyesters of lactic acid and glycolic acid, or a mixture of the two, degrade in the presence of water into the naturally occurring metabolites, lactic acid and glycolic acid. While the lactic acid degradation product that is released from biomaterials is well tolerated by the body, lactic acid can influence the metabolic function of cells; it can serve as an energy substrate for cells, and has been shown to have antioxidant properties. Neural precursor cells, a cell population of considerable interest as a source of cells for neural tissue regeneration strategies, generate a high amount of reactive oxygen species, and when associated with a degradable biomaterial, may be impacted by released lactic acid. In this work, the effect of lactic acid on a neural cell population containing proliferative neural precursor cells was examined in monolayer culture. Lactic acid was found to scavenge exogenously added free radicals produced in the presence of either hydrogen peroxide or a photoinitiator (I2959) commonly utilized in the preparation of photopolymerizable biomaterials. In addition to its effect on exogenously added free radicals, lactic acid reduced intracellular redox state, increased the proliferation of the cell population, and modified the cell composition. The findings of this study provide insight into the role that lactic acid plays naturally on developing neural cells and are also of interest to biomaterials scientists that are focused on the development of degradable lactic-acid-based polymers for cell culture devices. The effect of lactic acid on other cell populations may differ and should be characterized to best understand how cells function in degradable cell culture devices.
Genetic Deletion of the Clathrin Adaptor GGA3 Reduces Anxiety and Alters GABAergic Transmission (2016)

Golgi-localized γ-ear-containing ARF binding protein 3 (GGA3) is a monomeric clathrin adaptor that has been shown to regulate the trafficking of the Beta-site APP-cleaving enzyme (BACE1), which is required for production of the Alzheimer's disease (AD)-associated amyloid βpeptide. Our previous studies have shown that BACE1 is degraded via the lysosomal pathway and that depletion of GGA3 results in increased BACE1 levels and activity owing to impaired lysosomal trafficking and degradation. We further demonstrated the role of GGA3 in the regulation of BACE1 in vivo by showing that BACE1 levels are increased in the brain of GGA3 null mice. We report here that GGA3 deletion results in novelty-induced hyperactivity and decreased anxiety-like behaviors. Given the pivotal role of GABAergic transmission in the regulation of anxiety-like behaviors, we performed electrophysiological recordings in hippocampal slices and found increased phasic and decreased tonic inhibition in the dentate gyrus granule cells (DGGC). Moreover, we found that the number of inhibitory synapses is increased in the dentate gyrus of GGA3 null mice in further support of the electrophysiological data. Thus, the increased GABAergic transmission is a leading candidate mechanism underlying the reduced anxiety-like behaviors observed in GGA3 null mice. All together these findings suggest that GGA3 plays a key role in GABAergic transmission. Since BACE1 levels are elevated in the brain of GGA3 null mice, it is possible that at least some of these phenotypes are a consequence of increased processing of BACE1 substrates.